LOCUS       DAOORV010000001        79485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_318_length_79485_cov_9.064787, whole genome shotgun sequence.
ACCESSION   DAOORV010000001 DAOORV010000000
VERSION     DAOORV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 79485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_79485_cov_9.064787"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="petA"
                     /locus_tag="PKV56_00005"
     CDS             <1..366
                     /gene="petA"
                     /locus_tag="PKV56_00005"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOX66215.1"
                     /translation="RTPEMLASLEGHDDQLVDPQSLRQQQPAYAQNPARAIKPEIFVA
                     VGICTHLGCSPSNAPKGGNNPGLPSDWAGGFFCPCHGSTFDGAGRVYKNKPAPTNLEI
                     PMHRYTSATRLLIGEDAAT"
     gene            378..500
                     /gene="cydX"
                     /locus_tag="PKV56_00010"
     CDS             378..500
                     /gene="cydX"
                     /locus_tag="PKV56_00010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HOX66216.1"
                     /translation="MWYFAWMLGVGFAVLLAVMNAMWGENEAARQAAGDEEDGM"
     gene            497..802
                     /locus_tag="PKV56_00015"
     CDS             497..802
                     /locus_tag="PKV56_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon YbgE family protein"
                     /protein_id="HOX66217.1"
                     /translation="MKTPPPAATAFAAVTAASNVHLPCLATALTIMAVGTVYPPLMAD
                     ASGHADHGMATVLLYAMCAGFVRGVGFVPHSLVWRWLFSGWACALALVTAAALQSMH"
     gene            825..1256
                     /gene="trxC"
                     /locus_tag="PKV56_00020"
     CDS             825..1256
                     /gene="trxC"
                     /locus_tag="PKV56_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOX66218.1"
                     /translation="MNLVCPACGTTNRVPDARLHDQPVCGRCKGALMAAHPVELDDAR
                     LAAFVAGTGLPVLVDFWAEWCGPCKMMAPHFANAATQLPDVRFVKIDTEANPQASALN
                     QIRSIPTLILYRDGREVARQSGAMPAADLVRWIRSQLGRDA"
     gene            1266..2204
                     /locus_tag="PKV56_00025"
     CDS             1266..2204
                     /locus_tag="PKV56_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX66219.1"
                     /translation="MIIEAAAIAASAWGLHAWSHHAILNGLRAPRLTHGRAPDNPDVP
                     RQAWREVVIPGSNSRQLFGWMVRPASGSGQPAPAVLVMHGWGANASMMWPVVRPLHEA
                     GFAVLLIDARCHGLSDNEAFSSMPRFAEDIAAGLGWLRGQTEVAAHRVALLGHSVGAA
                     AAMLHASRHHDVSAVVSLSAFAHPREVMLRLLAEHRVPYPLIGWYVLHHVQRVIGARF
                     DDIAPLTTLRGVRCPVLLVHGIADSTVPFSDAQRLQSVSSWARLLPVNGDHDLRDTLA
                     LHGGALVEFLLQADAQARLREDGGNDDHPLPWRSAA"
     gene            2225..2941
                     /locus_tag="PKV56_00030"
     CDS             2225..2941
                     /locus_tag="PKV56_00030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX66220.1"
                     /translation="MTLNTPLLPLIDDELDVLSSLVPVAGQRIIELGCGAAALARALL
                     ARHPDSRVTGLEVDERQQAKNQAAPQAGLEFVSAGAEAIPFADASFDLALMLKSLHHV
                     PTERMAQALGEAARVLRAGGHLYVSEPIFDGPLNDLVRRFNDEGVVRAAAQAAVDTAL
                     RTGCWAAVAEQRFEMPVQFSDFADFERRMMRPTFADHRIDDAKLAEVRDAFESHVAAH
                     GAAFTRPMHVRLLRRQSDAN"
     gene            2931..3395
                     /locus_tag="PKV56_00035"
     CDS             2931..3395
                     /locus_tag="PKV56_00035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOX66221.1"
                     /translation="MRTDPESHRVGSLLVALQFSLIAALIALATPAFASGRAPAGAWL
                     LALGGLLLVGWAFFCNRPGNFNIHPAPRAGGRLVRHGPYRWVRHPMYTAVFCCALSSA
                     WASPSRWGWLGAAALVGVLATKARLEERWMVEAHAGYADYQARTWRFVPGLY"
     gene            complement(3414..3563)
                     /locus_tag="PKV56_00040"
     CDS             complement(3414..3563)
                     /locus_tag="PKV56_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HOX66222.1"
                     /translation="MHALTDLFTSDAGLMSLAVIAFTLGMAGFFIRYTLKHVREDEAA
                     AHVKR"
     gene            3766..4209
                     /locus_tag="PKV56_00045"
     CDS             3766..4209
                     /locus_tag="PKV56_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOX66223.1"
                     /translation="MNLDWNHFTPWASLAGGLLIGVATALYLLGDGRIAGIAGIVSAP
                     LRALLTGGNLAAERVRLLFIGGLLIAPWAWQLFAPLPEATVDAGPVAIVVAGLLVGIG
                     VRMGNGCTSGHGVCGLSRFSLRSLVNVLAFMGAGAAAVFVARHVL"
     gene            4219..4632
                     /locus_tag="PKV56_00050"
     CDS             4219..4632
                     /locus_tag="PKV56_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66224.1"
                     /translation="MRELIALLSGLIFGLGLIVGGMTDPGKVKGFLDVAGLWDPSLAL
                     VMGGAIAVGVFAFAAARRRTTAWSGDRIDIPSNTTIDARLIGGGLLFGAGWGIGGFCP
                     GPALVAMSSGLGSAAIFVVAMLAGMALHDRFLAPR"
     gene            4664..5752
                     /locus_tag="PKV56_00055"
     CDS             4664..5752
                     /locus_tag="PKV56_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX66225.1"
                     /translation="MLVFRQLFDPTSSTYTYLLGDSAAGEAVLIDPVFEHARRDAALL
                     GELGLRLVATLDTHVHADHVTGAWSLKQRCGSQIMLSEASAAVHADRLLRHGDRVKFG
                     SRHLEARATPGHTNGCMTYVLDDQSMAFTGDSLLIRGCGRTDFQQGSAAALYQSVHEQ
                     ILSLPAGCLLYPGHDYRGLTVTSVAEEARYNPRLGGEVNASDFAGFMNNLGLPHPKLM
                     DIAVPANLRCGRSEGDMAAPADPDWAQLTFSFGGLWEIQPHDLEERLASTAANAVQVI
                     DVREPSEFSDQLGHIRGARLVPLSQLTSRLPEIDRSRPVVTVCRSGARSAQASVLLGK
                     SGFEQVANLAGGMLRWRAQSLPVENGAG"
     gene            complement(5758..6789)
                     /locus_tag="PKV56_00060"
     CDS             complement(5758..6789)
                     /locus_tag="PKV56_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOX66226.1"
                     /translation="MPQSQTVNRRIVLASRPAASVGPEHFRLEEQPVPVPAAGQVLLR
                     TLCLSLDPYMRGRMSDAPSYAPSVQLGEVMTGGAVSRVQASQHPDFRPGDLVMGYTGW
                     QDYALSDGSGLTRLDADMPHPSLALGVLGMPGFTAFMGLLDVGQPRPGETVVVAAASG
                     AVGSVVGQIARLKGCRVVGIAGGADKCRFVVDELGFDACLDRRSGDLPAQLAAACPKG
                     IDVYFENVGGAVFDAVLPLLNTHARVPVCGLMSLYGGAPSAAAVDRLPALTRALLVKR
                     IRMQGFIIFDYEGRQNEFLGPMRAWLAEGKVKFREDVVDGLENAPQAFMGLLKGENFG
                     KLVVKVADA"
     gene            6889..7521
                     /locus_tag="PKV56_00065"
     CDS             6889..7521
                     /locus_tag="PKV56_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOX66227.1"
                     /translation="MHTFAQALFGLLFLAMPAIGHAIEEPSYEVVRKIDDVEVRQYAP
                     YIVAEVQIEADADEAGNLAFPILAGYIFGKNKGKQEFAMTAPVTQAAAPVRLEMTAPV
                     TQAAASGTPGSHVVQFVLPRGVTLQTAPEPLDARVRLREMPAQQVAVIRYSGFWSDAN
                     YKEHLEKLRATLKAADLTPSGEPVFSRYNPPFTPWFMRRNEIWLALPSVR"
     gene            7602..8486
                     /locus_tag="PKV56_00070"
     CDS             7602..8486
                     /locus_tag="PKV56_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOX66228.1"
                     /translation="MIRIELHVELSYEVDAQGADFVFNIHAAKTRCQTIAAESLVISQ
                     LVPSQIHTDPSTGNRYLRLRALPGQLKLSYMATVDITHHVADPAQLAEVPVHLLPPDV
                     MGYLYPSRYCQSDRLVKFAINEFGTLWRGHSRVEAIQAWVRRHVTFTSNTSNSNTSAV
                     DTLIEQVGICRDFAHVMIALCRALSIPARFTTATDYGADPVLGPPDFHAYVEVYLGDR
                     WYLFDPSGTTIPMGLVRFGTGRDAADVAFATIFGSVVTHAPVIHAWAVDDPSRGMTMP
                     FRCLEALSTDGGAPSAAS"
     gene            8534..9097
                     /locus_tag="PKV56_00075"
     CDS             8534..9097
                     /locus_tag="PKV56_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOX66229.1"
                     /translation="MAYGADFALHGMAVATLAAALMLAGPVDRWMPLASIAFAGLLAW
                     TALEYALHRFVLHGVRPFSDWHGQHHLRPVALICAPTVLTALLFVVGVFLPALALFDV
                     WIACAWTLGLLSGYLAYAVTHHATHHWRADSSWLKRRKRWHALHHHHTEQPAFYGVTT
                     AQWDHLFGSTGQPHRKDRDPDGRWRRA"
     gene            9307..9549
                     /locus_tag="PKV56_00080"
     CDS             9307..9549
                     /locus_tag="PKV56_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66230.1"
                     /translation="MHTPLIAAPRWSTASFGDATDTSPMELSALGEHLDRCHASKGRL
                     FDARCAAEAMHGFVASRFVTTLVVASLLIGISSLIL"
     gene            9553..18126
                     /locus_tag="PKV56_00085"
     CDS             9553..18126
                     /locus_tag="PKV56_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOX66231.1"
                     /translation="MVPYELRRLIPDAAPALCAMLNGARGRIEAPIRSEIFGLQRFAQ
                     HGRSLGETHRAAPASVRSAPFFPRLRNNIQTLREAHAYIGIQARTGYDISPAAEWLLD
                     NFHLIEAQLEEIHAGLPRRYFRALPVLLDEPLAGLPRVYGVAWAFVAHTDGAFDEELL
                     IQFLRAYQETRELTLGEMWALPTTLRVVLVENLRRLAERVATNKAAREVANLCCDRID
                     SASVDALDDLLALMARRGVERVFLGQMSLRMQDGRTTAQGGNRASDHADARAAQHEWL
                     RRALPDPAAIQAQHRADQTADNLSVSNAVTSLRAIGDADWTDIVARSSTLMHLMLSSP
                     VFEAEHAVTRDRTLHGIERLARKSGRSEVAVARTLLDLMQPAQGEAVALGGETSTAVA
                     SHWLSGAGRPALMHALGLSERAAASWRAVAHRLALPAYLGSLLFVTLGLVAWLLRHGG
                     GFDVSTGLMWLAAALMLFPASEAVVAVINRLISESARPAHLPRLALTAGIPAEHRVMV
                     VIPAMLTDTASTRALVHRLQLHHLANPERHAQFALLTDWADAGSARVGTDDALLAEAQ
                     QMIEALNTRHPTCYQAGRDELTARTAAPPRFILLHRERRYSETEQQWIGWERKRGKLE
                     QLITLLAEASDRPSTDALPAFIDLGEASRIALDTKYVVTLDSDTQLPPGRLHDLVGVA
                     AHPQNQPRIDATGRAVASGYAILQPRIVTPLPSPKDVTLYHWLFAGQCGIDPYSAATS
                     EVYQDVFGEGTYTGKGLLHVQAMHAVLSGRLPEGRVLSHDLLEGSIARCAAVTDITLI
                     EDSPFHADVAASRVRRWTRGDWQLLPFLLDSLSTDSRYRLRAINRWKMFDNLRRSLVA
                     PMSLALLLLALGGSVMSPWAALALVLAAFSAGPLMGAVAGFSPSRDDIAQRHFYSQAA
                     SDLARALCGGAWLLVQLPAQSMQAVDAIARAVHRMAVSGKHLLQWTTAAAAQARARTA
                     LPALLRQHWREPVLALALLAALVAVGTPTLWLAIGLCLSWAAAPVWTWWVSRPRPACA
                     EEALSAEDQRYFEVVARDTWRLFERCVVAADNHLPPDNLQTLPYDIVAHRTSPTNIGL
                     YLLSTACARQFGWIDTAELITRMEATLATMSRLQRHRGHFLNWYDTQTLAPLLPMYVS
                     TVDSGNLSGHLLAVAQACLEWARDPHHRPTPQTEARLQALAEQCEAIAWQADFSFLYH
                     HKRHLLHIGYRVAEQQLDAGFYDLLASESRLTSLLAIAKGDVPVRHWSSLGRMFYAVG
                     SEAGLRSWSGSMFEYLMPTLILDEPHGSVLHGACHAALREQIAFALVQSVPGRSVPWG
                     ISESAYAGQDHTLAYQYSPQGVPRLALRRTPPDELVVAPYATALAAQIAPHLARLNFA
                     ALETLAPRDHYGFIEALDFTASRKTGEEAFIPVSTFMAHHQGMTIVALANVLLGGVAQ
                     RWGMANAHIEAMASLLHERAPREVSMLYAPPAAPPPLAMQRRAPGLLRELFPGATALE
                     PTHVLSNGRYSVSLRANGSGWSRWGTTGITRSRDDALRDAQGSFFYLRRTGAEHAGEA
                     TPAASPLFSITQHPAPDPAAHYRSMFHADRVCFDAAWSEVQAHTTVWVSPEDDIEFRQ
                     IELRNLGDDVLDIELISAFDVTLADPRADEAHPAFSNLFVRAEWQGEQQALLFERTPR
                     LATERGLQAAHFLADTDPQVVSVRLQTDRQHWLGRNRSTHQPQASFEHLPADDIGTQA
                     ALDTGLDPVCAMAVRLRIEPQGKAMLTFATTASDNGGTLRAVIDKYRQTSHVKRASLM
                     SATLAGIRFRTLRISTENFDAIQTLTTALVLTLSRPRARAASLNDLAAGVCDRRLIWR
                     FGISGDRPILLVSAGAPEGLGLIRSLAQALRLWSWSGIACDLVVVNAEPASYLMAVQR
                     ELSAMRERHGADSGAEFRPAGATGPSSTGLHVLRADEISADELSTLQSLARLRLQADG
                     RPLTHHVQEWVQLHEEAFEQRHDTSTVPVSVSDGVGPAVPAAAGSFAASSGEFRFAVS
                     ARSRPMRPWANVLANPSFGTQLTEAGAGYTWALNSRMNQLTAWSNDPVGDPPSEWFLL
                     QDLKTLDAWSIAPSAWGDDSVTYRIAHGQGYSVISHRRGDVEVTASWCVDPQSSVKQV
                     RLRVLNRGHRTLQLRMVGIAEWMMGANRSDRATAHTALHRQRLPDLNAPNGRSDPGRS
                     QKLTALMCTQHERSAGFGDGTAFLAIAGPDERDDKLENGGDWTCDRRECFDARGRLVL
                     PDHFGQQGGSGLDPCAALSTRVRLLAGESAERVFLLGYADSPDAARQLAARAATVPAG
                     QRLEAVRETWDALLEATTVRTPDPLFDALVNRWLLYQTVSCRLWARAGFYQAGGAYGF
                     RDQLQDAMALTWAAPGMLRDQILLGASRQFAQGDVQHWWHAPGGAGVRTHFSDDLLWL
                     PHACVRYLRTTGDTGVLDESIAFIEGAPIPDGAEDAYYTPSVSTEQASVYEHAARAID
                     RSLRVGAHGLPLMGTGDWNDGMNRVGHEGRGESVWLGWFLCELVDDFAPLARERGDDA
                     RAKAWEAAALGWRNALNGIAWDGQWFKRAFFDDGQALGSHVNPEASIDLIAQAWAVLS
                     GAASPEMQREAMHSVEARLVDHEAGLIKLLDPPLAHAQPSAGYIQAYPPGVRENGGQY
                     SHAGVWALMAQARLQTDGADNGARSSPPDIDLPYRYFSFLSPAHRAAHPTRGAAYGLE
                     PYVMAGDVYTQAPYVGRGGWSWYTGAAAWLHRSAIESIFGLQQGARTLSFTPCLPSHW
                     PQAELTLKRGQGQACRTMRFILVRPAALAELEATAPWGAQILLPGESLAWQDLAPHAC
                     FVIPLPALTTKELPGDLPASADRIAAPAI"
     gene            18267..21449
                     /locus_tag="PKV56_00090"
     CDS             18267..21449
                     /locus_tag="PKV56_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOX66232.1"
                     /translation="MNPPAVRLGHGRVLGVLLLMALAYAVVGRVSLLLAIPPAYASPL
                     YPPAGMALVFVLLYGRSAAAAVAVGAIVINLLLNPLRAVGLAEVVIGLGAGLQALVAA
                     ELVQRMVRQPLTLSAPRDIARFYLFGALIGCLVSASVATAALAVADVVPASAWPMTWL
                     TWWMGDALGTLIAAPILLTLAGRPREAWAPRRRTVGLTLGIVTALLVATTVLVAHWDR
                     ERVQTSFERDAAAASMAAQAQLNTPLHALEALRGLYIGSNEVNPGELRAAAAYWLNRD
                     GDLQAVGYQERVRLVDVPALEAQLRDAGFPDYRVLDRTATDERVPTTMPEAYAVRLVE
                     PREGNVGLLGINTLSIPAARQAIEASVRTDRPVASASFHLSQDAVGSPKTGVVIYRAI
                     YRGQPATEEERAAAVRGVVFVTVRVEDLLRSVLAQAPAYLHFCVTDTDTSARLRQLAA
                     TSLCAEAPDALVHRQVIAFAGRQWEWRVNASEVGASQAAGARHYAWLFSSVGLMTAAV
                     LGALLLTVTGRARIIELAVQDRTAALEQQVRERAQVEAAMRESEQRFRNIFNNVPIGV
                     LYTDIDGKVRQANPRFCELVGRDAATLSRLSTRDFLHPDDVDEDAALFARLLAGEIAI
                     YRRQTRYVAPDGHVVWVQRSVSLLRDETGRPQRIVSVAEDITETLRLAEAVRAREAAE
                     ASNRAKSEFLSRMSHELRTPLNAMLGFAQLLELEDRPALGRAQRAWVGQIQQAGWHLL
                     EMINDVLDLSRIESGNLRMELETLDLAELLAATRPLVQQSADLRNIVITEDLARGANF
                     VTGDATRVKQILTNLLTNAVKYNVDGGRIQLSTRVQGDWVELMVTDTGLGMSSEQMAS
                     LFQPFNRLGRELTGTEGTGIGLVISQRLAELMGGELTAASHVGRGSSFMLRLPAAGNP
                     GTVPSDLDGLAADRNDYQQRHVHYVEDNETNVEVMRGILAQRPQVQLDVSVTGLDGLA
                     AIRARRPDVVLLDMHLPDISGLELLRHLKLDPGTRSIPVVAVSADALPAQIDDAFMAG
                     AHRYLTKPVNVSELLSVLDELLSQADTGFE"
     gene            21517..22464
                     /gene="rpoH"
                     /locus_tag="PKV56_00095"
     CDS             21517..22464
                     /gene="rpoH"
                     /locus_tag="PKV56_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOX66233.1"
                     /translation="MTAMNMTISNASNTSALALRDPWAMVPSLGSLDAYISAANRVPM
                     LTLEEEQSLARQLRDKGDLQAAGQLVLSHMRLVVSIARQYLGYGLPHGDLIQEGNVGL
                     MKAVKRFDPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQ
                     SLKDDAVAGETHRSTLTQGEVDTMARTLNVKREEVLEMETRLSGGDVPLEPLTDDGEE
                     SYAPIAYLADDTHEPSQVLEAQRRDWLSSDGISLALDSLDARSRRIVEERWLKVNDDN
                     SGGMTLHDLADEYGVSAERIRQIEVAAMKKMRKALGASH"
     gene            complement(22534..23097)
                     /locus_tag="PKV56_00100"
     CDS             complement(22534..23097)
                     /locus_tag="PKV56_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOX66234.1"
                     /translation="MALLGCDAPSSGGTAIAFKGIDITGAEYARSLSLTDQNGQARTL
                     ADFKGKVTVVFFGFTQCPDVCPTTMAELAQVKAALGKDGDRIQGVFVTVDPDRDTAEV
                     LKGYMSGFDPSFVALRGTPEQTAATAKEFKVFYAKVPGKTEGSYSVDHTAGSYVFDAQ
                     GKVRLFIRYGSGSQALAADLKTLLALG"
     gene            complement(23181..24092)
                     /gene="cyoE"
                     /locus_tag="PKV56_00105"
     CDS             complement(23181..24092)
                     /gene="cyoE"
                     /locus_tag="PKV56_00105"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOX66235.1"
                     /translation="MSETLAPSLPLARRSSRLKQFYALTKPRVVQLIVFCAVIGMLLA
                     VPGLPDIKLALAATAGIWLVAGAAAAFNCVVEQHIDAKMARTSWRPTAKGELTNAQTL
                     TFSAVLCSIGSVLLYTMVNPLTMWLTFATFVGYAVIYTVLLKPATPQNIVIGGASGAM
                     PPVLGWAAIRGEVGAESLILCLIIFLWTPPHFWALALYRAEDYRKAGLPMLPITHGAA
                     FTQLHVFLYTLVLFAATLLPFISGMSGWIYLVSAIALGAGFTGYAWKLWRNYSDALAR
                     KTFRFSIWHLMLLFAALLIDHYLDPLL"
     gene            complement(24111..25274)
                     /locus_tag="PKV56_00110"
     CDS             complement(24111..25274)
                     /locus_tag="PKV56_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOX66236.1"
                     /translation="MNPPAYDLGPVPTLVLVGAVLALAPVLWVAWRYRRADAARRRQA
                     LTLITLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYGSASPLGALQAIGAAQTAMPTGP
                     VTHGKAWIEMIHRYLATGVGALITWLAVAAWLDARKFRHGLPARLSPWWATLTWVWVC
                     GQGAFGALTVTMKLYPAIVTLHLLGGLGLLALLSVQSQAQQPPLPMSRAMFRGTVLVF
                     LVSVAQIALGGWVSTNYAVLACTDFPTCQGAWWPPMDFEHGFTVARELGAGKDGGYLP
                     FAALTAIHMAHRLGAAVVVVAIGALAWRLRASGQPELRRWAGVLAVVVAWQLISGLSN
                     VVLDWPLVAAVAHTGGAAALVVVLTTMLSRAYRAHPLSPAQAVPPSAALGAAS"
     gene            complement(25322..26038)
                     /locus_tag="PKV56_00115"
     CDS             complement(25322..26038)
                     /locus_tag="PKV56_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66237.1"
                     /translation="MSGSNSPTLGSDRSDAPEQVSPLSFTVHSMPTPDLSARRTAAGR
                     LKMLAVLAICSAPVIAAYIIYFVIGLEGRTTNYSEFVTPTREMPETLPLRNLAGQPVL
                     AASLRGQWLIVVVADGACDAGCQSHLLLQRQVQEMLGNDKPRVDKVWLIPGEAAPRAE
                     LLKAIAPGRASHVLQVPGEALSQWLKPSDGHRLDEHVYLVDPKGQWMMRTPVGPEPAK
                     LKKDLERLLRASASWDQPGR"
     gene            26494..26712
                     /locus_tag="PKV56_00120"
     CDS             26494..26712
                     /locus_tag="PKV56_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HOX66238.1"
                     /translation="MKYLVGLAFVTIIGALAYAGVSMVRDGRDGKPKTHSMVNALAFR
                     VALSILLFVCLLGSYKMGWIQPTGFLRR"
     gene            complement(27031..27405)
                     /locus_tag="PKV56_00125"
                     /pseudo
     CDS             complement(27031..27405)
                     /locus_tag="PKV56_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
     gene            complement(27411..27893)
                     /locus_tag="PKV56_00130"
     CDS             complement(27411..27893)
                     /locus_tag="PKV56_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HOX66239.1"
                     /translation="MLKRNCSLTPRQLLAVFGGFCVLSLMIAGFFWLQGAKLIMPFAW
                     IELAAVGIALMVYSRHATDQENIAMRSGRLTVELTSGSMVEKAEFATEWVSVEPVTGD
                     QSLIELSGQGRRIEVGRFVRPEMRRQLANELRQAVRLARTRPQDALLAAESGQREQSR
                     "
     gene            complement(28137..29051)
                     /locus_tag="PKV56_00135"
     CDS             complement(28137..29051)
                     /locus_tag="PKV56_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX66240.1"
                     /translation="MAVASHTTQRLIDEAAVAATQRRLGRLPTPPWLHAEIAGRMAER
                     LALFRMSPDRMIDWGAFLGAGSALLTAAYPQAALRAVEPSEDLALRSRQALRSPWWSG
                     RRWRAPPVEVVVDSNEVQPTGAQLLWANMALHAVKDPPALLQRWQRMLAGGGFVMFSC
                     LGPDTARELRALYAGQGWPAPTVDFVDMHDLGDMLLQAGFADPVMDQETLTVHWESAQ
                     ALCKDLRLLGGNASPGRFGGLRTPRWLTRLYRELDSMRDQNGRLGLTFEIIYGHAFKA
                     DSPVQVAAETQVSLEEMRRLMREARRPA"
     gene            29072..29803
                     /locus_tag="PKV56_00140"
     CDS             29072..29803
                     /locus_tag="PKV56_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOX66241.1"
                     /translation="MNVQPRLIDPDERPWWRRLPGLCAVCHGWGRGRVCGTCISRFAP
                     HNDRCLRCAATLTARVELCGDCIRNPPPFDSALAAVDYGHPWNRLIREFKFNDSPDLA
                     GPLAELLVAAHRRRTHAPPDLLLPVPLGSGRLKERGYNQSWEIARRCAHRLGCRTDAT
                     LLLRIKDTPHQLSLPREHRKRNVQGAFAVEPTRRDALRGRNVTLVDDVMTTQATAAEL
                     TKVVRAAGAAAVHVWVVARTPAPGH"
     gene            29806..30276
                     /gene="trmL"
                     /locus_tag="PKV56_00145"
     CDS             29806..30276
                     /gene="trmL"
                     /locus_tag="PKV56_00145"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOX66242.1"
                     /translation="MFNIVLVHPEIPPNTGNVIRLAANTGCELHLIEPLGFSMDDKLL
                     QRAGLDYHEHARVQRHASWDDFIDNRQPDLTRCFAFTTRGSQTPGAVRWQAGDWLVFG
                     SETSGLPESLRASFAQDRLVRLPLLPGQRSLNLSNAVAVAVFEAWRQHGYAGGV"
     gene            complement(30284..31273)
                     /locus_tag="PKV56_00150"
     CDS             complement(30284..31273)
                     /locus_tag="PKV56_00150"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOX66243.1"
                     /translation="MNLSVLGAGAWGTALAVSASARHPTMLWARDPAQAQGMQALRRN
                     MRYLDAVELPPSIHITSDFKAAVEHARGGLLIVATPMSALAAVLSALPAGPQGPGLFW
                     LCKGLQKDTGRLGHEVAGQVRPGARVGVLSGPSFAEEVARGQPTALVAASVDPSLCSQ
                     AVELLHCDNLRIYTSDDPIGVEVGGAVKNVMAIATGLADGLQLGYNARAALITRGLAE
                     MVRLGTALGARAETFMGLSGLGDLVLTATGDLSRNRTVGLQLAQGKPLPQILADLGHV
                     AEGVFSAATVLRRAQALGVEMPITSAVVAVLEGRVTPSQGVELLMGRDPRAEL"
     gene            complement(31312..31797)
                     /gene="secB"
                     /locus_tag="PKV56_00155"
     CDS             complement(31312..31797)
                     /gene="secB"
                     /locus_tag="PKV56_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOX66244.1"
                     /translation="MADEDNNAPVFNIQRIYLKDMSLEQPNSPAIFLEQTPPKVDINI
                     GVGAETVGPDNYEVTLRVTVTATVGEKTLFLLEAKQAGIFEIRNIPKEQMQPIVSIAC
                     PGIVYPYLRAIVSDLCTRAGFPPVLLTEVNFQAMYEGQKAAAAEEAAKKAAANGGALP
                     N"
     gene            complement(31917..32198)
                     /gene="grxC"
                     /locus_tag="PKV56_00160"
     CDS             complement(31917..32198)
                     /gene="grxC"
                     /locus_tag="PKV56_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HOX66245.1"
                     /translation="MSTSSIPLVAVTMYTTQVCPYCLRAKALLKERGVTVIDEVRIDL
                     DPSARDAMVERTGRRTVPQIFIGDAHIGGCDDLIDLDRRGGLLPLLSGH"
     gene            complement(32214..32624)
                     /locus_tag="PKV56_00165"
     CDS             complement(32214..32624)
                     /locus_tag="PKV56_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX66246.1"
                     /translation="MTFFLANWFLFAAALVSGALLFWPMLSRGTGGVRVSPSEAVNLI
                     NREKGVLIDVSEAAEYAAGHAGGARNIPLGSLEASADLPKNKAVPLLLLCPTGARAQR
                     GVGLLRKRGYENTHAVAGGVAAWREANLPIERSV"
     gene            32730..33425
                     /gene="gpmA"
                     /locus_tag="PKV56_00170"
     CDS             32730..33425
                     /gene="gpmA"
                     /locus_tag="PKV56_00170"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOX66247.1"
                     /translation="MYQLVLIRHGQSTWNLENRFTGWVDVELTPLGVDQAQAAGRLLK
                     KHGFDFDLAYTSVLKRAVWTLWHTLDQLDRTWLPVVHSWRLNERHYGALEGLNKADTA
                     EKYGDAQVLQWRRSYDTPPPALDPSDPRCERFDRRYASLKAGEVPLTECLKDTVERVL
                     PFWNDAIVPSISAGKRVVVAAHGNSIRALMKHLDNISDDDIVGLEIPNGVPIVYDLDA
                     QFRPISRRFLEID"
     gene            complement(33441..34127)
                     /locus_tag="PKV56_00175"
     CDS             complement(33441..34127)
                     /locus_tag="PKV56_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX66248.1"
                     /translation="MLTCAVVFPFIDAPRRMAHVGRWSAQALTVLGIGLRVSGTPVSG
                     ASLFVANHVSWIDILAINAVRPVRFVSKADIRAWPLLGWLVACGGTLFIERERSRDAL
                     RVLHLVAQALKAGEQVAVFPEGTTSDGHGVLPFHANLLQAAIVAQAPVQPIALRFSDD
                     RETVSSAAAYIGDTTLLQSLWAVASADGLVARLSFLAAQPSADLERRALATRLSADIA
                     AQLPRMTPTV"
     gene            complement(34190..35485)
                     /locus_tag="PKV56_00180"
     CDS             complement(34190..35485)
                     /locus_tag="PKV56_00180"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOX66249.1"
                     /translation="MKILIQNGRLIDPASGRDEQTDIAIAAGRVVRIGQVSADFHPNR
                     TIDARGLVVAPGFVDLAVRLREPGHEHEGMLESEMAAAVAGGVTSLVCLPDTDPPLDE
                     PGLVEMLKFRARNLNQSHLYPLGALTRGLAGEVLTEMAELTEAGCVGFSHAEVALKDT
                     LVLHRALQYASTFGYTTWLRAQDGYLGNGVAAKGALATRMGLTGVPVIAETIALNTLF
                     ELVRDTRARVHICRLSSAAGVDLVRRAKAEGLPITCDVSVNHLHLTDIDIGYFDAAMR
                     LTPPLRQVRDRDALRAGLADGTIDALVSDHTPVDEDAKTLPFAEAEPGATGLELLLGL
                     ALKWGEQSGQELARTLATITISPVRVIADALGSLASSAGQLVEGGVADVCLFDPAEYW
                     QVRPDALRSQGKHTPFTGHELPGRVRYTLVSGHVAFESP"
     gene            complement(35505..36467)
                     /locus_tag="PKV56_00185"
     CDS             complement(35505..36467)
                     /locus_tag="PKV56_00185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOX66250.1"
                     /translation="MNFRNNPQLNKHGELIHLLSIEGLPRALIHQILDTAGTFLSVND
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIARSSAAKGESLLD
                     TIANLSAMHADMFVVRHSQSGAPYLIAQHCAPHVHVVNAGDGRHAHPTQGLLDMYTIR
                     HYKKDFTNLTVAIVGDIVHSRVARSDIHALTTLGVPEVRAVGPKTLVPGDLREMGVRV
                     CHDMAEGIRGADVIIMLRLQNERMSGAMLPSAGEFFKNYGLTQEKLALAKPDAIVMHP
                     GPINRGVEIDSRVADGAQSVILPQVTFGIAVRMAVMSTIAGNSA"
     gene            complement(36464..36970)
                     /gene="pyrR"
                     /locus_tag="PKV56_00190"
     CDS             complement(36464..36970)
                     /gene="pyrR"
                     /locus_tag="PKV56_00190"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOX66251.1"
                     /translation="MSSLQLDAEALYGDLLAGVRGLLRPQTVLVGIWSGGAWLAERLQ
                     RDLQLEGEFGVISSALHRDDFGSRGMTAGADHTRLPFEIEGRHIVLIDDVLFTGRTTR
                     AVVNELFDFGRPASVILAVLVDRGGRELPIHPAYAAARITLPAAQRLSLVRDDAGRFS
                     FDIRERTA"
     gene            complement(36967..37407)
                     /gene="ruvX"
                     /locus_tag="PKV56_00195"
     CDS             complement(36967..37407)
                     /gene="ruvX"
                     /locus_tag="PKV56_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOX66252.1"
                     /translation="MPDLGTIAPGASLGGAAQRLRTYLAFDFGQKRVGVASGNTLTRS
                     AQPLCTIAVTGEDRFAAIARLVGEWQPDALVVGVPFHPDGAEHENTLRARRFARQLHG
                     RLRMQVHEVDERYTTTEAHAGGARDLDAASAALILEQYLRSESE"
     gene            complement(37400..37978)
                     /locus_tag="PKV56_00200"
     CDS             complement(37400..37978)
                     /locus_tag="PKV56_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOX66253.1"
                     /translation="MSFGSINLTNQFLIAMPGIAGDTFAGTVIYLCEHTDKGALGLVI
                     NKPIDIKLKSLFEKVELELDRVDLAESPVYYGGPVQTERGFVLHEQLDDQEGRFSSSL
                     RIPGGLEMTTSKDVLEALASGNGPTKILVTLGYSGWGAGQLEDEMGRNTWINVEAEPE
                     IIFNTPVEQRYDKALSLLGIDPAMLSADAGHA"
     gene            38012..39505
                     /locus_tag="PKV56_00205"
     CDS             38012..39505
                     /locus_tag="PKV56_00205"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOX66254.1"
                     /translation="MTPSLDAALVWFRRDLRAEDQAALHHALRAARHVWCVFVLDRDI
                     LDPLPLADRRVEFIVDSLTDLHARLAEIGAVHGVTGAGLIVRHACARDEIPALAQRLR
                     VQAVYASHDDEPRALERDAQVRGRLAHLGIALHTSKDHLIFERSEVLTGSGTPYGVFT
                     PYKNAWLKRLTPEHLAPWSIEPHAAHLAALPKDLPGGPPSLTDIGFAPTNLASLRMGQ
                     GATGAQVAFNEFLTRIDGYEDARNFPAVKGPSYLSVHLRFGTISIRQLAREAHRRMQD
                     GSRGAEVWLSELIWRDFYHQVLHHHPHVVARSFKPEYDRIVWEHGKHADALFTAWCEG
                     RTGYPLIDAAMAQINQTGYMHNRLRMVVASFLVKDLGIDWRWGERYFAEHLNDFDLAA
                     NNGGWQWASSTGCDAQPYFRIFNPVSQSEKFDPAGKFIRRYLPQLAALPDAALHAPWT
                     ARPVDLAAAGIEIGRNWPAPVVQHDAARARTLERYAVVKANRPSDRT"
     gene            complement(39502..40533)
                     /locus_tag="PKV56_00210"
     CDS             complement(39502..40533)
                     /locus_tag="PKV56_00210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX66255.1"
                     /translation="MIPFADHIAELTSHRDRELLDLALASSLMDMVRPRMVTIHRCVG
                     EVEDRRWLNRAHLRRGDAAPKSDLPWSDLENLPRLEPGEPRFEALTEQRLVIVGGAPG
                     RAYFPLVGGSDAVGVIEIETDAALDETAQRTIEGVLRIFHNFDRLLDYSERDTLTGLL
                     NRKTFDESFLKATSDLMPDTDAEKAGRRCCGGDPCGYWIGVIDIDHFKSVNDRFGHLI
                     GDEVLLLLSRLMARSFRLHDRLYRFGGEEFLVLMRCPAEDDAAQAMERFRVLTEQFEF
                     PQVGRVTVSIGFTAVRPGDTPSGAFERADQAVYHAKQNGRNRVCSHADLVAQGLLADT
                     TKVGDFEFF"
     gene            complement(40559..46825)
                     /locus_tag="PKV56_00215"
     CDS             complement(40559..46825)
                     /locus_tag="PKV56_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOX66256.1"
                     /translation="MDSLRNPQSTDSRGDDPESQSDLSALSWVQDELRRSLELAHKAL
                     RRHLKESETTFDSDVDAADPAVIHGARQHIHQGVGALELVGLPAGANLLRACEAAVQR
                     CIAKPRMLDIHAVATIESASFAVLDYVARLLSGKTVSPLAMFPQYRAVQELAGADRVH
                     PADLWTRDWQWQPLPDDELAVPRAPDAATRSAIEGKMLSIMRNPTPATASRMSDICAG
                     LGAGTDHPQTSTLWKLAAAMFEAQSQGLLSPDVFSKRVASRLLAQLRIFERGGREVSE
                     RLARDLLFFCAQADSPGDGRRAPRLAAVRQAYGLVHHLTTDYHVSALGRFDPAVIAQA
                     RKRVAAAKESWSGVAGGEMHRLNGLAEQFALAGESLKRLFPSGELLAEELRAAAAQAQ
                     QSAAPPSAPLAMEAATSLLYVEACLEDAEFDHPELGARVQRMSQRMASVRQGEAPQPL
                     EGWMEDLYRRVSDRQTMGSVVQELRATLSEAEKLIDQFFRNPADPEVLIPVPNHLSAM
                     RGVLSVLGMDHAASALLRMRDEVDGLSSTEVDVARVPQAGVFDRLADNLGALGFLIDM
                     LSVQPTLAKTLFTYDADSGTLSPVMGRGERKPVIPGEPAPAVAEPRLIEQAQMLAFNA
                     AREDVSIEAVTRDLERLSHEATAADQPALAATVNQARMALEKAQDSDAVSAARDQLSE
                     ALADFVHTSSEAVGLEPATTAGRDVGADESVEDEEMREVFLEEALEVFETARGAVAEL
                     AAQPADLQQLTIVRRAFHTLKGSARMVGLTEFGDAAWVCEQLYNTRLSEQQAADSSLL
                     DFTAWSLDQFADWVGDIAASRATSHTANRIREAAAQLQAGAEAPVIEAVPALPPVVHE
                     LAFAAADDADSGAVPPDFTMPADFELDMPGEVPAAESMPEVLALDELDFDALVDLDLN
                     LPATGVESSPEVAFDSDSTMLLRDPAKAGPADSRLLALDLDASEAQSMFEPFDPEATQ
                     PLPHDSLLSRDELSEAVDSPSFELLNLDLGSEPFSVFDEEQGEAAPEFQPEPVALLEA
                     EPGIGQASELIETVDETPDADEAVKVIGSLRIGIPLFNIYLNEADELSRRLCTELAEW
                     AMELDRPVGDTAVALAHSLAGSSATVGFADLSNLSRTLEHALSRCCAIGSGTSEEGAL
                     FVDAADEIRHLLHQFAAGFLKQPSEALLARLADHELSSAQRLDTAVAELDAESLLDSE
                     GAPLDPPIHLDFSVSTDVPRLDPLGVAELKSLDESPVPDSESIRSAQEGAPQPFDDDE
                     DIDAIDAIDAELFPIFEEEGEELLGELAIRLQDWVRQPDDASQPAGAMRTLHTLKGGA
                     RLAGAMRLGELVHRLESRIENLMSQSAEAALTADDVEPLLGRCDVLRAAFEAVQRAAA
                     STEEAAAPEAVAVTRPVEMAPPVSIDEEAAVEETEAVADYSAAQAAPAPVSAPGFDSD
                     HRPVDSAANAEVDWSRFGASEGVVEAPRAPLASISTAAVRVRAPLLDRLVNQAGEVSI
                     TRSRIASDVQEIKGSLSDLTENLDRLRGQLRDIELQAETQISARMEAAKVSAQAFDPL
                     EFDRFTRFQELTRMMAESVNDVATVQRTLQRSLQSTEDELAAQARLTRELQDDLLRTR
                     MVEFEGLSDRLYRVVRQAAKETGKQVRLDIVGGSIEIDRGVLDRMTGAFEHLLRNSVT
                     HGIESPEARVAAGKDATGGVVVAVSQDGNEVSVEVRDDGRGLDLERIRAKGVATGLIT
                     EGAALSDGDLANLIFTAGFSTADKVTELSGRGVGMDVVRSEVVAIGGRIETSTTSGKG
                     SSFKLVLPLTTAVTQVVMVRCGDLTVGIPSPLIVSVRRVPAAEMEQAHLSGSFTVDDS
                     ALPFFWLGALLQTSSCSAEPAGRSAQVLVIRSASQRIVIHVDDVVGNEEVVVKNLGPQ
                     LSRMPGLAGMTLMASGDVVLIYNPVALATLYGSSARVATQSALQSRLAGHAQPAADES
                     PARTLAPLVLVVDDSLTVRRVTERMLLREGYRVMLAKDGLDALEQLAKERPAIVLSDI
                     EMPRMDGFDLVRNLRADARLSDLPVIMITSRIAQKHRDYAAQLGVEHYLGKPYSEEDL
                     LALIARCTAVPVVA"
     gene            complement(46893..47933)
                     /locus_tag="PKV56_00220"
                     /pseudo
     CDS             complement(46893..47933)
                     /locus_tag="PKV56_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(48587..49105)
                     /locus_tag="PKV56_00225"
     CDS             complement(48587..49105)
                     /locus_tag="PKV56_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX66257.1"
                     /translation="MANKEALRELQHRLAERLQEARSTLRGRSWLAVECGTRGFLFPL
                     KEAGEIFPMTALAPVSHAHPWFLGVANLRGHLHGVVDLAGFFGIKSGTDHRDQAMLVG
                     FNASLDVNAALLVDRLAGLRGEEQLTLDSQAPAGSPSFVGQRLRDVDGRVWQEISLAD
                     LVRHAAFLKIVG"
     gene            complement(49193..49558)
                     /locus_tag="PKV56_00230"
     CDS             complement(49193..49558)
                     /locus_tag="PKV56_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66258.1"
                     /translation="MAIKNILLVDDSKTELYHVSGLLTKQGYAVRTAENGEEAMRRLE
                     EQTPDLILMDVVMPGQNGFQLTRAITRDPRFSRVPVIMCTSKNQETDKVWGIRQGARD
                     YLVKPVNADELAAKIRAFG"
     gene            complement(49640..50056)
                     /locus_tag="PKV56_00235"
     CDS             complement(49640..50056)
                     /locus_tag="PKV56_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66259.1"
                     /translation="MNEDTGADAPKARVLVIDDSNTIRRSAEIFLKQGGYDVYLAEDG
                     FDALSKVNDHAPALIFCDILMPRLDGYQTCAIIKRNARFADVPVIMLSSKDGLFDKAR
                     GRMVGSQDYLTKPFTKDQLLGAVRLHLGEDAPAVVD"
     gene            complement(50111..50284)
                     /locus_tag="PKV56_00240"
     CDS             complement(50111..50284)
                     /locus_tag="PKV56_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOX66260.1"
                     /translation="MTETRTWMCLICGWIYDEAVGDEEHGISPGTRWAEVPMNWTCPE
                     CGARKEDFEMVQI"
     gene            50505..51443
                     /locus_tag="PKV56_00245"
     CDS             50505..51443
                     /locus_tag="PKV56_00245"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOX66261.1"
                     /translation="MTAQTQPTADAADELQEAPAPACVMSFNASDATGAGGVSGDVAT
                     IAAMGAHGLPVITSIVMRDTAEVFDHHALEPDVVAEQARCVLEDITIAAWKVGFLGSA
                     EGVGAVAEILSDYPDVPLVAYMPNLSWLDEDQQQAYLDAFRELVLPQTEVLIGSHQTL
                     TDFLLPDWDGERPTSPRELAVAASEHGANFVLVTSVLVTKGKSADQYLDNVLASPQGA
                     VTGEKFERFDASFNAAGDTLSAALASLLASGSELHTAVGEALAFLDQALDAGFRPGMG
                     NVVPDRFFWALAPGEEDEGGEGDPSETETPGTRHIH"
     gene            51496..52788
                     /gene="hemL"
                     /locus_tag="PKV56_00250"
     CDS             51496..52788
                     /gene="hemL"
                     /locus_tag="PKV56_00250"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOX66262.1"
                     /translation="MTSTNAQLFERAQRVIPGGVNSPVRAFRAVGGTPRFIARAQGPY
                     IFDAEGTRYIDYIGSWGPMILGHGHPAVLDAVQKAAMDGFSFGAPTEREIELAEEILK
                     LVPSMDQVRLVSSGTEAAMSALRLARGATGRDKIVKFEGCYHGHADALLVKAGSGLAT
                     FGNPTSAGVPEDVVKHTVVLEYNNVAQLEEAFALHGDGLACVMIEAICGNMNFVRASV
                     PFMTRLRELCTQYGTLLVFDEVMTGFRVALGGAQSLYAKLIPGFAPDISVFGKVIGGG
                     MPLAAFGGRRAVMEQLAPLGPVYQAGTLSGNPVATACGLATLREIQKPGFFDALTAST
                     RQLVDGLNAAAHASGVPMVTDSEGGMFGFFFASKLPQNYTQVLATDKARFNHFFHAML
                     DRGVYLAPALYEAGFVSSAHRPDDLLATATAAKQALQS"
     gene            52826..54211
                     /gene="mpl"
                     /locus_tag="PKV56_00255"
     CDS             52826..54211
                     /gene="mpl"
                     /locus_tag="PKV56_00255"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOX66263.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHQVTGCDSGVYPPMSDQLRA
                     LGIELVEGYGADQLGVNPDLFVVGNVVTRGNPLMEAVLNAGAAYISGPQWLAEHVLQG
                     RHVLAVAGTHGKTTTTAMLAWILEQAGLQPGFLVGGVPLNFGVSARLGSGAPFVIEAD
                     EYDTAFFDKRSKFVHYRARTAVLNNLEFDHADIFENLAAIERQFHHLVRTVPLQGRLV
                     VNARDEAIQRVLGMGCWSDVVRFGARKETPGELRARGEPHAFDVLRGSMKIARVEWSL
                     AGEHNQLNALAAIAAADHVGVPADVAAKALASFENVRRRLELRGSAAGISVYDDFAHH
                     PTAMRTTINGLRRQVDARWNPATQPRPRILAVFEPRSNTMKLGTMKAQLPWSLEEADL
                     SFCHSGGLDWDAAQALAPLGKLAAVCDSIDQLVSRVAATAQPGDHVLCMSNGGFGGVH
                     QLLLDALARKA"
     gene            54211..54852
                     /locus_tag="PKV56_00260"
     CDS             54211..54852
                     /locus_tag="PKV56_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOX66264.1"
                     /translation="MPATASPAITHLLYLHGFRSSPHSKKARQFGDWVRAHRPDLHWW
                     CPQLPPSPSDALALLIEGTKDWPLAHAAVVGSSLGGFYATVLAERWARSDERPDGDSV
                     RVALLNPAVHPARDLAAHIGETTSWHDGDRFFFRPEFIDELQQLSPDQITQPQRYLAV
                     IAKGDEVLDWREMSSRYADCSIRLLEGSDHALSDFDDHLKATIDFLGLEAGAA"
     gene            complement(54853..55059)
                     /locus_tag="PKV56_00265"
     CDS             complement(54853..55059)
                     /locus_tag="PKV56_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66265.1"
                     /translation="MRPFDWVMASILGLTGGISLLCFGVFIATGIDLWLKRTRMFRRW
                     AFAVALLWFNIVVWGSVVKTIINW"
     gene            complement(55121..55306)
                     /locus_tag="PKV56_00270"
     CDS             complement(55121..55306)
                     /locus_tag="PKV56_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66266.1"
                     /translation="MLIFRWVLLLLLVAGLVCLAMYVGTGQMRYRQLGITIIKWAVIA
                     ALGFFAVLILERFVVAV"
     gene            complement(55306..56241)
                     /gene="lpxC"
                     /locus_tag="PKV56_00275"
     CDS             complement(55306..56241)
                     /gene="lpxC"
                     /locus_tag="PKV56_00275"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOX66267.1"
                     /translation="MLAQRTLKSLTRAVGVGVHGGQRVELTLRPAPADSGVVFRRVDL
                     PQPVDIPVHAHSVCDTRMATALSPCGDPGAPKVNTVEHLLSACAGLGIDNLIVDITAE
                     EVPILDGSAASFVFLLQSAGIVLQDAPKRFLRILKPVEVREGEGPSLKWARLEPFDGY
                     KLTFEIAFDHPAVNQTGQHVVFDMGSGQYKREIARARTFGFTKDVELMRSRGLGLGGS
                     LDNVVVVDDYKVLNSGGLRYDDEFVKHKILDAIGDLFIIGHPLIGAYSAFKSGHALNN
                     KLVRAVLAEPGCYDIVTFGDASKAPAGLAELAPAW"
     gene            complement(56319..57533)
                     /gene="ftsZ"
                     /locus_tag="PKV56_00280"
     CDS             complement(56319..57533)
                     /gene="ftsZ"
                     /locus_tag="PKV56_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOX66268.1"
                     /translation="MAIEMIEEFDLGTQIKVIGVGGGGGNAVDHMIAQGVQGVEFVNA
                     NTDAQALNRSTAHHLIQLGTTGLGAGAKPEAGKAAALEADERIRQAISGAHMLFITAG
                     MGGGTGTGAAPVIARIAKEMGILTVGVVTKPFDFEGNRRMKAADAGLAELEANVDSLI
                     VVLNEKLLEVLGDDVTQDQAFAEANDVLKNAVGGISDIIHMHGMVNVDFEDVKTVMSE
                     PGKAMMGTAQASGPDRATKAADAAVACPLLEGIDLSGAKGVLVLIAANRSTLKLSESK
                     NAMNTIRRYAAEDAHVIFGTVYDEGLGDKLRVTVIATGLSPARRQQAPISVVHSAPMQ
                     RTGTDNLPILNNPLNAPGLGMQGGHNSPDYGSLSTPSVWRTRTAAAKVDALASNGMDE
                     IEIPAFLRKQAD"
     gene            complement(57753..58982)
                     /gene="ftsA"
                     /locus_tag="PKV56_00285"
     CDS             complement(57753..58982)
                     /gene="ftsA"
                     /locus_tag="PKV56_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOX66269.1"
                     /translation="MAKELKDLVVGLDIGTAKVMVVVAELLPGGELRIAGLGSAPTHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKITRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPVDVARVVETAKAINIPNDQRLLLVQAQEFVIDGHEVKEPIGMSGGRLEVKVHIVT
                     GAQSAAENIVKCVRRCGLEVEQLVLNPSASSHAVLTEDEKDLGVALVDIGAGTTDVAI
                     FTDGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVEFGVAKQLLADPSDQLEVP
                     GLGDRAPRMLSRQALAGVIEPRIEEIYSLVHQVIRESGYEELLSSGIVICGGAAVMPG
                     MVELGEDIFLKPVRKGIPLYHGALQDMVANTRSATVMGLLEEGRIARSRGLRAAQQAG
                     SVKTVLGRARDWFLGNF"
     gene            complement(58996..59838)
                     /locus_tag="PKV56_00290"
     CDS             complement(58996..59838)
                     /locus_tag="PKV56_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOX66270.1"
                     /translation="MSSTPFVRSMSAARDRASVPALPADVRWMRYTANGLFVLAALLL
                     GATALHLLTRQPVFALRSIGIEGDLSRNTVATIRVNAVPKLRGNFFTTDLQVDQRAFE
                     SVPWVRRAVVQRVWPNRLKVRLEEHRPAALWRGGGATDQLVNTMGEVFEASVGDVEEE
                     GLPVLEGPEGSSAGMLAMLSRLQSVFGRLDRRVESLRLSSRASWRAELDSGAKVELGR
                     GSEDEVVARAERFVGTVSQVTEQFQAPLVYADLRHRDGYALRLRGITTTLNPGTGPGA
                     GNRN"
     gene            complement(59846..60850)
                     /locus_tag="PKV56_00295"
     CDS             complement(59846..60850)
                     /locus_tag="PKV56_00295"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOX66271.1"
                     /translation="MNVDPKALGKVAVLMGGTSAERAISLMSGHGVLSALQSLGVDAQ
                     SFDPSERDLSDLKKLGIARCFIALHGRHGEDGTVQGALELMGIPYTGSGVMASAVAMD
                     KVMTKRIWLSEGLPTPRHVWLPPGEHDIEQVRRVPGQLGLPLIVKPPREGSSIGVTKV
                     TGGGDVDRQMLDAVVLAAKYDADVLCEEFIDGEEVTCPVIGEGADAHALPVIRIRAPE
                     GAYDYQNKYFTDAVQYMCPSGLPESEEREIQRLALASYRTLGCRGWGRADLMIRAADR
                     QPFLLEMNTSPGMTSHSLVPMSARSVGIGYDQLCLLILSQARLDGPAAPVSAATDVKA
                     "
     gene            complement(60847..62301)
                     /gene="murC"
                     /locus_tag="PKV56_00300"
     CDS             complement(60847..62301)
                     /gene="murC"
                     /locus_tag="PKV56_00300"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOX66272.1"
                     /translation="MKHAVKHIHFVGIGGAGMSGIAEILHNLGYRVSGSDQSDSATSR
                     RLASLGIRVAVGHDASNIDGAEAVVTSTAVKADNPEVTAARARRVPVVPRAVMLAELM
                     RLKKGIAIAGTHGKTTTTSLVTSVLAEAGLDPTFVIGGRLNAAGANSRLGSGDHIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMDTYGHDFARLKQAFVEFVHRMPFYGAAIVCSD
                     DPGVRSIMPMLSRPVVTYGFGADAMVRAVDVQARAGRMRFTVQRRNGIAMPDLDVTLN
                     LPGDHNVLNALAAIAVATELELPDAAMVQGLADFRGVDRRFQRYGEVRATAAADESAG
                     SFTLIDDYGHHPVEMAATLSAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVIGSAD
                     AVLLTEVYAAGEPPIVAADGRALARALRVAGKVDPVFVDDVAAMPQAILDHARAGDVV
                     VSMGAGSIGGVPAQVLDLQRRTDAAAAVTMEGSR"
     gene            complement(62298..63380)
                     /gene="murG"
                     /locus_tag="PKV56_00305"
     CDS             complement(62298..63380)
                     /gene="murG"
                     /locus_tag="PKV56_00305"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOX66273.1"
                     /translation="MTQGKHLIVVAAGTGGHVIPGLAVAKEMQQRGWTVSWLGTPTGM
                     ENRLVPPTRIPLDTVAFTGVRGKGLFHTLTGGIRMLGAFWACLGIIRRRRAQVVLGMG
                     GYLCFPAGLMASLLGKPLMLVNADAALLMSNRALLPVADRVAFGFDGPAAQGARHGVV
                     TGNPVRAEIESLPAPTTRFAGRDGPLRLLVVGGSLGAQVLNRCLPQALALMPPAQRPR
                     VTHQTGAAHAESVQAEYAGATVDAEVLPFIDDMAGRLADCDLIVCRAGAITVSELCAA
                     GVASVLVPLVVSTTSHQRDNAQWMASHGAALQLPQNELTPRHLADVLAGLTRDSLLAM
                     ASRARALARPRAASRVADELEGLIAA"
     gene            complement(63487..64755)
                     /gene="ftsW"
                     /locus_tag="PKV56_00310"
     CDS             complement(63487..64755)
                     /gene="ftsW"
                     /locus_tag="PKV56_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOX66274.1"
                     /translation="MTTLRLPRFGSLRARVSGLWRSDASESTAIPVRDWINVSSGAPA
                     RLLAFDQALLCIVLALLALGLVMVYSASIAMPDNPKFTLYSPTHFLVRHLVSLLIAVL
                     AALVAVQVPVSFWEKSAPWIFVGALILLLLVLVPFIGKGVNGARRWIPLGFLNFQPSE
                     LAKLAMAMYAAGYMVRKMDARENFWQAVLPMAFAVAVVGLLLLAEPDMGAFLVIAMIA
                     MGILFLGGVNGRMFLLITSVLIGAFVLMITLSDWRRERIFAYLNPWDEKYTLGKAYQL
                     SHSLIAFGRGEFFGQGLGASVEKLHYLPEAHTDFLLAVIGEELGFVGVACVIVAFFWL
                     SRRVFHIGRQAIALDRVYAGLYAQGIGIWMGGQAFINMGVNLGVLPTKGLTLPLMSYG
                     GSAILMNMIALAIVARVDIENRQLMRGGRA"
     gene            complement(64752..66317)
                     /gene="murD"
                     /locus_tag="PKV56_00315"
     CDS             complement(64752..66317)
                     /gene="murD"
                     /locus_tag="PKV56_00315"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOX66275.1"
                     /translation="MNHLQGLVVLVLGLGDSGLAMARWCARCGATVRVWDSRAVAPNA
                     QALAEHVPQATVLGGELADAAFEGVRLVLKSPGLSPTDARIAPPLARFVERGLLVQGE
                     LELFARALADLKADRGYAPRVLAITGTNGKTTTTAMTALLVERAGRQVAVAGNIGPTM
                     LQTLADAIDGDSLPQVWVLELSSFQLDEAKGFEPDAAAVLNLTEDHLDWHGSMENYAA
                     AKARVWGREAVMVVNRDDPRVEALARSVQPADIPAKGTVRSRAPKPAERQIVRFGLDA
                     PQCPGDFGLVSENGMAWLVRALESDDSFPRKRVRAGTEVEAEELHIQRLMPADALRVR
                     GRHNAANAMAALALATAIDCPLAPMLHGLREYAGEPHRVAHVATIEGVDAYDDSKGTN
                     VGATVAALEGLGADRAPSRIVVILGGDGKGQDFTPLAEPVRRHARAVATLGRDAALIE
                     AALSGIDGLVLKRHATLEAATRWAHEQAKSGDAVLLSPACASLDMFRNYAHRAEVFVD
                     EVHAIAADAGVAA"
     gene            complement(66331..67509)
                     /gene="mraY"
                     /locus_tag="PKV56_00320"
     CDS             complement(66331..67509)
                     /gene="mraY"
                     /locus_tag="PKV56_00320"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOX66276.1"
                     /translation="MLLSLAQWMQTIYPELGFFRVFQYITFRAVMAAMTALLIGLAFG
                     PWVIRRLTALKIGQPIREYGIKEHLSKSGTPTMGGVLILIGITVSTLLWFDWSNRFVW
                     IVLLVTLGFGAIGWVDDWRKVVDKNPEGMSSRDKFFWQSLIGLVAAFYLAFSVSETSN
                     LRVLELFVRWVQSGFSNDLPPTADLIVPFFKTISYPLGVYGFIVLTWFVIVGASNAVN
                     LTDGLDGLAIMPVVMVGSALGVFAYVIGNAYFSKYLLFPHIPGAGELLVFCAAMAGAG
                     LAFLWFNTYPAQVFMGDVGALSLGGALGTIAVITRQEIVLGVMGGVFVVEALSVMMQV
                     SWFKYTRKRYGTGQRILKMAPLHHHFEKSGWKETQVVVRFWIVTMLLCLVGLASLKLR
                     "
     gene            complement(67499..68896)
                     /gene="murF"
                     /locus_tag="PKV56_00325"
     CDS             complement(67499..68896)
                     /gene="murF"
                     /locus_tag="PKV56_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOX66277.1"
                     /translation="MTLAHAHDLLPGSCLFGDGATPLKRVHTDSRSLRAGDLFVALRG
                     ERFDAHDFLPQARAAGAAAVLAERDVAQAGLPGLQVGDSRLALGALAAGWRRGFDIPL
                     IAVTGSNGKTTVTQMIAAILRAWQGEGAFSTEGNFNNDIGVPLTLLRLRQDDTTFHRA
                     GVVELGMNHPGEIAQLAAMAAPTVALVNNAQREHQEFMSGVEAVARENGSVIEALGAD
                     GVAVFPADEEFTPLWRALAGDRRVLTFALGDVAADVSATASWSADHWLVALHAAAGSR
                     RLKLHIAGLHNVKNALAATACALAAGCPLDAAVRGLEAFKAVQGRSQAGSFVRDGRQV
                     TLIDDSYNANPDSVRAAIEVLAGLPGPRWLVLGDMGEVGDQGPAFHREVGGFARDAGM
                     DALWTAGSLCAHAAAAFGAARHFDSVDDLLAALPQAPVATSVLVKGSRFMKMERIVAA
                     LKSAAAPSTGGSHAA"
     gene            complement(68896..70452)
                     /locus_tag="PKV56_00330"
     CDS             complement(68896..70452)
                     /locus_tag="PKV56_00330"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOX66278.1"
                     /translation="MALQTLGSVPAALSWLAQQGARGLATDSRRLEPGDAFIAWPGQA
                     QDGRRHVQPALAAGAPACLVEAEGAEAYGFDDARVAAMDGLKATTGVLASAFYGAPSR
                     RLSVIAITGTNGKTSTAWWTAQALSVLGLRCGVIGTLGIGAAPVNPGAAAAPALQATG
                     LTTPDPVTLQRAFRRFADDGFSACAIEASSIGLAEQRLSGTHIDVAVFTNFTQDHLDY
                     HGSMDAYWQAKADLFRWPGLRAVVLNVDDPQGAALQQALDGSGLDVWTCALAGPAAAS
                     DSARLAARSVRYVDGGLAFDLVESGEPSVPVRTALIGDYNVSNLLEVVASLRALGVPL
                     HDAARACSTLAPVPGRMQRVTLGLQADQELPEVLVDYAHTPDALDKALRALQPLAAAR
                     GGALWCVFGCGGNRDAGKRPLMGALAQRLAGHVVVTSDNPRDEVPGSIISAIVSGMGG
                     QPQPQVIEDRRAAIAHAVARAGSSDVVLIAGKGHEAHQDIAGVSHPFDDASAARAELQ
                     ARASVAEKQP"
     gene            complement(70452..72269)
                     /locus_tag="PKV56_00335"
     CDS             complement(70452..72269)
                     /locus_tag="PKV56_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOX66279.1"
                     /translation="MRQGGWNARAGAVAKPVAGHARSINYASSPLLASRTPASRSKVL
                     VALVGLGFCVLLGRALYVQVIGSSFYLKQGEARYARTLDLSANRGRIFDRNGQLMASS
                     VPAPSLWAIPKDFAADSRERRQLARLLGMTPKALDDRLDDNPNFVWLKRQVDETQAKD
                     ALALGLPGVHQLREVKRKYPEGEAAAHVVGFTDAENRGQEGMELAFQKELTGRNGTRR
                     VIKDRLGKVVEDIGDSVAPVDGRDIQLSIDSKIQYFAYRRVRDAVAENKAKAGSVVVL
                     DAQSGEVLALANYPSYAPSDRRNLTGEQLRNRAMTDTFEPGSTVKPFIAAWALETGRV
                     RPDTIIHTAPGRIAMGGFIISDAHPHGDLTVAEVIQKSSNIGSMRMGLMFQPREMWDL
                     YTAVGFGQKPQIGFPGSVRGRLPSAKAIAKDETMHATMCYGYGLSASLFQIAHAYTVF
                     AHDGEMIPVTLMKSEAQGAEARVPGLRVISPQTALAVRQMLALVTGPGGTAPKAQTVG
                     YSVGGKSGTAYSHEGKSYDNKKYRSWFVGLAPIKNPRIIVAVMVDQPTAGKWYGGDVA
                     APVFSDLVQNTLRTLGVQPDLDVKTQIVARDVPGEDESF"
     gene            complement(72266..72631)
                     /gene="ftsL"
                     /locus_tag="PKV56_00340"
     CDS             complement(72266..72631)
                     /gene="ftsL"
                     /locus_tag="PKV56_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOX66280.1"
                     /translation="MGGAACIAKGARVTRLNLLLLLVLLVSSVYLVRVSYDAHHLYGE
                     LDRARNQQRQFETEYGRLQAERQTQAAPARVEKTARDKLRMVQATPAIMKQVTVTADA
                     GRASSAVGPGLAASSRGQP"
     gene            complement(72666..73682)
                     /gene="rsmH"
                     /locus_tag="PKV56_00345"
     CDS             complement(72666..73682)
                     /gene="rsmH"
                     /locus_tag="PKV56_00345"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOX66281.1"
                     /translation="MTTAPASAHTTVLLDEAVDALLHAAPPGAADDEQLARAADGVYV
                     DGTFGRGGHSRLLLQRLGARARLLAIDRDPEAVAAAAGDPDFADPRFSIHHTSFGQMV
                     PTLAAAGVSRIDGLLLDLGVSSPQIDSPERGFSFRFDGPLDMRMDTTRGESAADFLAR
                     ADERHIAEVIRDYGEERFAVQVAKALVARRESGNPVRTTRELSEVVAGAVKTREPGQN
                     PATRTFQALRIFVNAELEELEQGLSAALELLAPGGRLVVISFHSLEDRIVKTFIARES
                     KAVFDRRAPFAPEVAMRLTALGRIKPGETELRANPRARSAVMRVAERTDAASAPPPPA
                     RSRR"
     gene            complement(73693..74178)
                     /locus_tag="PKV56_00350"
     CDS             complement(73693..74178)
                     /locus_tag="PKV56_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOX66282.1"
                     /translation="MRCDTVGLGALFWVFVANLVFQGPAALTLDSKGRLTMPARHLSV
                     LRALEVTELTITRHPDGPLMVFPRPTWEEFRDRINALPMEAVRWKRMFLGNAMDVDLD
                     GSSRVLIAPELRKSAGLDRDVMLIGMGSHLELWDAVQHASHEAEVVKGDMPEALKGFT
                     F"
     gene            complement(74401..74970)
                     /locus_tag="PKV56_00355"
     CDS             complement(74401..74970)
                     /locus_tag="PKV56_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HOX66283.1"
                     /translation="MSMLILGLVIFLGVHSTSIFAPAWRNRQAAALGDIAWKGIYALL
                     SLVGFALILHGYGQARGAPVLLYQPPAALRHVAWLLMLPVFPLLLASYLPGRISAITR
                     HPMLVATKLWALAHLLANGTLASTVLFGAFLAWAVADRISLKHRVARSVPGAPPGRYN
                     DAIALTVGLCLYAAFIGAVHLWLIGVSPR"
     gene            complement(75048..78404)
                     /locus_tag="PKV56_00360"
     CDS             complement(75048..78404)
                     /locus_tag="PKV56_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX66284.1"
                     /translation="MLTLIIRAIGQLPVGRKLLLIYLLDLTAVIYVSSILISEKYISI
                     DFSRKEVAGNAYIAAVRDVLVALPTPLPPAKAGDPGADAAATRLDRVEWEPRLAALRE
                     AESRHGQGMKSADVSTAFAAALQTAMNRPVFDAGQLAASMAAGRELITRIGNQSNLIL
                     DPDLDSYYTMSLVLLRFPELQELLALTVHKAVELDQSPADQRNKVHTELLILEGRLDA
                     VLKGIDSDYAEALAAGPPTLRAALAPARDRLTAAVASFRQHPMHIADRGAADRREVLA
                     ARHRELVAELHATWRAATVGLEGLLDARIQGLFVRMWTHLGTAVALLMLILSIVYFVA
                     RQIAQPLQRLADVAGRVSATGDYTMRAAHESRDEIGQLVQAFNGMLGDLDRERVVRED
                     LAATSRAEEAQRALLESFPMPLIVTSIPEHRVLHANQPAQPWLQGVTTDPWSLRLEPH
                     ARARFFSRLSDTDRVDGFEVQWQSGEASAGGSRASWALLSARRLTYQGQPALLTVFTP
                     IGQIKMLEQRLKLWAKVFEASSESIVIFDVERRILTANTAFSRDSGWGAEEVVGQTPE
                     FLYSSRHDADFYETLWQSAVIRGMWQGEVWLKRKTGEAYPTWLVANAVRDSDGRITHF
                     VAAGADITEHKASEERIHHLAHHDVLTDLPNRSLCLERLRMAVEQAGRTGQHVGVVFI
                     DLDRFKNINDSMGHHVGDALLRSVSRRLIQAVRAGDTVSRLGGDEFVVVLNGVSSAEE
                     VASIVHERMLPLVREAHTVEGVQLHVSCSAGMAVYPQDGRDIDHLMRHADAAMYRAKS
                     GGRNQALFFTPEFHIQAQERLAIENELREVTARGELLLHFQPRVDAASGGVVGLEALV
                     RWQRPEHGMVSPATFIPIAEETGLIVPIGAWILGEACRQHAAWRDAGLGVIPISVNVS
                     AIQLRDSSLPAQLREALGTHGVDPSAIEIELTETFLMENAIATVESLERLKDIGVTLS
                     IDDFGTGYSSLNYLHQFPIDKLKIDQSFVRDILEDPADWAITKAIIGLGHTLGLRVVA
                     EGVERLEEANWLRDAGCDELQGYLFSKPMPPAQIEDWLQGRPLVPPAPARTRLGSMPR
                     TTTVTAVAPNAQPDSDAKDDPLSQAAALMS"
     gene            complement(78506..79417)
                     /locus_tag="PKV56_00365"
     CDS             complement(78506..79417)
                     /locus_tag="PKV56_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HOX66285.1"
                     /translation="MDAIRERLAEKLGTSKVSESATPGVVKVVSHSAAAPAAPVSRAM
                     LTPVSNSTRVIGASPTHAARLEAAQLKAAAAGHQSAAHWSYEGAGGPESWGALQPEFA
                     TCSTGTRQSPIDITDGIKVELDAVQFNYRPSNFRVIDNGHTVQVNVAVGNTIEVTGKR
                     YELVQFHFHRPSEERINGKPFDMVVHLVHKGVEGKLAVIAVLLDRGSAQPVVQNVWNN
                     LPLEKGDEVMAKGQIDLMALLPTDRRYFTYMGSLTTPPCSEGVLWMVMKTPVEISPEQ
                     LSIFSRLYPMNARPIQSASGRIIKESN"
BASE COUNT        13452 a        27023 c        26129 g        12881 t
ORIGIN      
        1 cgaacacccg agatgctggc ttcgctggaa ggacacgatg accagctcgt cgaccctcag
       61 tcgctgcgcc agcaacagcc ggcctatgcg cagaacccgg cacgcgccat caagccggag
      121 atcttcgtgg cggtcggcat ctgcacccac ctcgggtgct caccgagcaa cgcgccgaaa
      181 ggcggcaaca accccggcct tcccagcgac tgggccggcg gcttcttctg tccgtgccac
      241 ggttcgacct tcgatggcgc aggccgggtc tacaagaaca agcctgcgcc gaccaacctc
      301 gagatcccga tgcaccgcta cacgagtgcc acacgcctgc tgatcggcga agacgctgcc
      361 acctgaggag atgggccatg tggtatttcg cctggatgct tggggtcggc ttcgcggtgc
      421 tgctggccgt gatgaacgcg atgtggggtg agaacgaggc cgcgcggcag gccgccggtg
      481 atgaagagga cggaatgtga agacgccccc acctgcagcc acagccttcg cggctgtcac
      541 cgcggcgtcc aacgtgcact tgccgtgcct cgccacggcg ctgaccatca tggcggtcgg
      601 caccgtctac ccgccgctga tggcggacgc atccggccac gctgaccacg gcatggcgac
      661 ggtgctgctt tacgccatgt gtgcgggctt cgtacgtggc gtggggttcg ttccgcacag
      721 cctcgtgtgg cgatggctct tctcgggctg ggcctgcgca ctcgcactgg tcaccgcggc
      781 tgcgctccaa tcaatgcact gacccccaca tggagccttg caacatgaac ctcgtctgtc
      841 ctgcctgcgg caccaccaat cgcgttcccg acgcgcgact gcacgaccag ccggtgtgcg
      901 gtcgctgcaa gggcgcgctg atggcggccc atcccgtcga gctcgacgat gcgcgtctgg
      961 ctgccttcgt cgcgggcacc ggcctgcccg tgctggtcga tttctgggcc gagtggtgcg
     1021 ggccctgcaa gatgatggcg ccgcacttcg cgaacgccgc gacccagctg ccggacgttc
     1081 gcttcgtcaa gatcgacacc gaagcgaatc cgcaggccag tgcgctgaat cagatccgca
     1141 gcatcccgac gctgatcctg tatcgcgacg gcagggaagt ggccaggcaa tcgggcgcca
     1201 tgcctgccgc cgacctggtc cgctggattc gatcccagct cggccgcgat gcctgaggcg
     1261 attcgatgat catcgaggcg gcggccatcg ccgcaagtgc atggggcctg cacgcctggt
     1321 cgcaccatgc catcctgaac gggctgcgcg caccccgact gacccacggg cgcgcacccg
     1381 acaacccgga tgtgccgcgc caggcctggc gcgaggtggt catcccgggg tcgaattcaa
     1441 ggcagctgtt cggctggatg gtgcggcctg catcgggctc gggccaaccg gctccagcgg
     1501 tcctggtgat gcacggttgg ggtgccaacg cgtcgatgat gtggccggtg gtgcggccgc
     1561 tgcatgaagc cgggttcgcg gtgctgctga tcgacgcgcg gtgccacggc ctcagcgaca
     1621 acgaggcgtt cagctccatg ccgcgcttcg ccgaggacat cgcagccggg ctgggctggc
     1681 tgcgcgggca gaccgaagtc gcggcccacc gtgtggcgct gctcggtcac tcggtcggtg
     1741 cagcagctgc catgctgcac gcctcgagac accacgacgt cagcgccgtg gtcagcctgt
     1801 ccgcattcgc gcatccgcgc gaggtgatgc tgcgcctctt ggccgaacac cgggtgcctt
     1861 atccgctgat cggctggtac gtgctgcacc atgtgcagcg cgtgatcggc gcgcgtttcg
     1921 acgacatcgc ccccctgacc acgctgcgcg gcgtgcgatg cccggtgttg ctggtgcacg
     1981 gaatcgccga cagcacggtg ccgttttccg acgcgcagcg cctgcaatcc gtgtcatcgt
     2041 gggctcgact gctgccggtg aatggagacc atgatctgcg agacacgctg gctctgcatg
     2101 gtggcgcgct ggtcgaattc ctgctgcagg ccgatgctca ggcacggctg cgggaagatg
     2161 gcgggaacga tgaccatccg ctgccctggc gatctgccgc ctgacctgac cgaggacccc
     2221 tgccatgacg ctgaacacgc cgctgcttcc gctgatcgat gacgaactcg acgttctgtc
     2281 cagcctggtg ccggtggccg gccagcgcat catcgaactg ggctgcggtg ccgcagcact
     2341 ggcgcgagcc ctgctggcaa ggcatcccga cagccgggtg acaggcctcg aggtggatga
     2401 gcggcagcag gcgaagaacc aggcggcgcc tcaggccgga ctggagttcg tctccgccgg
     2461 cgccgaggcg atccctttcg cagatgccag cttcgacctg gcgctgatgc tcaagtcgct
     2521 gcaccacgtt cccacagagc gcatggcgca ggcgctcggc gaggcggctc gggtgctgcg
     2581 cgccggcggg catctgtacg tgtcggaacc gatctttgac gggccgttga atgacctggt
     2641 ccgccgcttc aacgacgagg gcgtggttcg cgctgcggcg caggctgcgg tggacaccgc
     2701 cctgcgcacg ggctgctggg ctgcggtcgc cgagcaacgc ttcgagatgc cggtgcagtt
     2761 cagcgacttc gccgatttcg agcggcggat gatgcggccg acctttgccg atcaccgcat
     2821 cgacgatgcc aagctggccg aggtgcgcga cgccttcgag tcccatgtcg ccgcccacgg
     2881 agcagcgttc acgaggccga tgcacgtgcg cctgctgcgc aggcaatccg atgcgaactg
     2941 accccgagag ccaccgcgtc ggcagcctgc tggtcgccct gcagttctcg ctgatcgcag
     3001 ccctgatcgc gctggccacg cccgcgttcg caagcggccg ggcccccgca ggcgcctggc
     3061 ttctggcctt gggcggattg ctgctcgtcg ggtgggcgtt cttctgcaac cgcccgggca
     3121 acttcaacat ccatcccgcg ccccgcgcgg gcggccggct ggttcgacac gggccctacc
     3181 ggtgggtccg ccacccgatg tacacggcgg tcttctgctg cgcgctgtca agcgcctggg
     3241 cgagcccgtc tcggtggggt tggctcggcg cagccgccct ggtgggcgtg ctcgcgacga
     3301 aggcccggct ggaggagcgc tggatggtcg aagcgcacgc aggctatgcc gactaccaag
     3361 cacgaacctg gcggttcgtg ccgggcctgt attgagacgc ggcttgagtg cgctcagcgc
     3421 ttcacgtgcg cggcagcctc atcctcgcgc acatgcttaa gcgtgtagcg gatgaagaag
     3481 ccggccatgc ccagcgtgaa ggcgatcacc gccagactca tcaggcccgc gtccgaagtg
     3541 aaaaggtctg tcaacgcatg catcggaatc tccctttgat caacacaggt ccattggatt
     3601 cctcgagcgg cgggcagacc ttgtcatggg tcaacatatc ttcacgacac tcaccggtac
     3661 agagaccgat cccgtaccct ctggtcgacc tcgcgatccg ccagcaggcg gccctgcacg
     3721 gggcctcgtc gctgccggga aactcaccat gaaagccccc tccccatgaa tctcgactgg
     3781 aaccacttca cgccctgggc atcgctcgcg ggtgggctgc tgataggcgt cgccactgcc
     3841 ctctacctgt tgggcgacgg ccgcatcgcc ggcatcgccg gcatcgtgtc ggcaccgctg
     3901 cgcgccttgc tgaccggagg caacctggcg gccgaacggg tccggctgct gttcattggc
     3961 gggctgctga tcgcgccgtg ggcgtggcag ctgtttgcgc cgctgcccga agccacggtc
     4021 gatgcggggc cggtggcgat cgtcgtggcc ggcctgctcg tcggcatcgg ggttcgcatg
     4081 ggcaacgggt gcaccagcgg ccacggcgtg tgcggtctca gccgcttctc gctccgttcg
     4141 ctggtcaacg tcctggcgtt catgggcgcc ggcgctgctg ccgttttcgt tgcccgccac
     4201 gtgctctgaa ggatgaccat gcgcgaactg attgcactgc tgagcggcct gatcttcggc
     4261 ctgggactga tcgtgggggg catgaccgac ccgggcaagg tgaagggctt cctcgatgtg
     4321 gccggcctgt gggatccgag cctggcgctg gtgatgggcg gcgcgatcgc ggtcggcgtg
     4381 ttcgccttcg ctgcggcccg gcgacgcacc acggcctgga gcggggaccg catcgacatc
     4441 ccgtcgaaca ccaccatcga cgcccggctg atcggcggcg gcctgctgtt cggagccggc
     4501 tggggcatcg gcgggttctg cccgggaccg gcgctggtgg cgatgagcag cggcctcggc
     4561 agcgccgcga tcttcgtcgt cgcgatgctg gcgggcatgg cgctgcacga ccggtttctc
     4621 gcaccgcgct gaggcgcctg ccccgcactg aaagggaagc tccatgcttg tattccgcca
     4681 gctcttcgat ccgacctcgt cgacctacac ctacctgctc ggtgactccg cggccggcga
     4741 ggccgtgctg atcgatccgg tgttcgagca cgcacgccgt gatgcagccc tgctgggcga
     4801 actcggtctg cgcctggtcg ccacgctcga cacccacgtg cacgccgatc acgtcaccgg
     4861 cgcctggtcg ctgaagcagc gttgcggcag ccagatcatg ctgtccgaag cttcggccgc
     4921 ggtccatgcc gaccgcttgc tgcgacatgg cgatcgcgtc aagttcggtt cacgtcatct
     4981 cgaagcccgg gccacgccgg gccacacgaa cggctgcatg acctatgtgc tggacgatca
     5041 gagcatggcg ttcaccggtg acagcctgct gatccgcggc tgcggccgga cagacttcca
     5101 gcagggcagc gcggctgcgc tctaccagtc ggtgcacgaa cagatcctgt cgctgccggc
     5161 cggttgcctg ctctatcccg gtcacgacta ccgcgggctc accgtcacga gcgttgccga
     5221 ggaagcgcgc tacaacccgc gcctcggcgg cgaggtcaac gcttcggact tcgcgggttt
     5281 catgaacaac ctcgggctgc cgcacccgaa gctgatggac atcgcggtac cggccaacct
     5341 gcgttgcggc cgctccgaag gcgacatggc cgcgcccgcc gatccggact gggcgcagct
     5401 gacgttcagc ttcggtggct tgtgggagat ccagccgcac gacctggagg agcgcctcgc
     5461 cagcacggcg gccaacgcgg tccaggtcat cgatgtgcgg gagccttcgg agttcagcga
     5521 ccagctcggc cacatccgcg gcgcccggct ggtgccgctg tcgcagctga cgtcacgcct
     5581 gccggaaatc gaccggagcc gcccggtggt cacggtgtgc cgatcaggcg cccgatcggc
     5641 acaggccagc gtgctgctgg gcaagtcggg gttcgaacag gtcgccaacc tggccggcgg
     5701 catgctgcgc tggcgcgccc agtcgctgcc cgtggagaac ggggcgggct gaagccttca
     5761 ggcgtcggcg accttcacca ccagcttgcc gaagttctct cccttcagca gtcccatgaa
     5821 ggcctgcggc gcgttctcga gcccgtcgac gacgtcctcc cggaacttga ccttgccttc
     5881 ggccagccag gcgcgcatcg ggccgagaaa ctcgttctgg cgaccttcgt agtcgaagat
     5941 gatgaagccc tgcatgcgga tgcgcttgac cagcagagcg cgggtcagcg caggcaggcg
     6001 gtcgacggca gcggctgaag gtgcgccgcc gtagagcgac atcagtccgc acaccggcac
     6061 gcgcgcatgc gtgttgagca gcggcaggac cgcatcgaac accgcgccgc cgacgttctc
     6121 gaagtacacg tcgatgccct tcgggcaggc cgctgccagc tgcgccggca ggtcgccgct
     6181 gcgtcggtcg agacaggcgt cgaagccgag ttcatcgacg acgaagcggc acttgtccgc
     6241 gccgccggcg atgccgacga cacggcagcc cttgagcctg gcgatctgcc cgaccaccga
     6301 acccacggca ccgctggccg ccgcgacgac cacggtctcg ccgggccggg gctggccaac
     6361 atcgagcagg cccatgaacg cggtgaaccc cggcatgccg agcacgccga gggcgagcga
     6421 cggatgcggc atgtccgcgt cgagccgagt gagtccgctg ccgtcggaca acgcatagtc
     6481 ctgccagccg gtgtagccca tcaccaggtc accgggacgg aaatccgggt gctgcgaagc
     6541 ctggacgcgg gacaccgcgc cgccggtcat cacctcgccg agctgcaccg acggcgcgta
     6601 ggacggcgcg tcgctcatgc ggccgcgcat gtacgggtcg agcgacaggc agagtgtgcg
     6661 cagcagcacc tggcctgccg cgggcaccgg cacgggttgc tcttcgagcc gaaagtgctc
     6721 cggccccacc gacgcggcgg gacgcgaggc gagaacgatc cggcggttga cagtctggct
     6781 ctggggcatg gcgaaatcct gggatggagg tacggggtcc gacgtggcgg gcagcatcgt
     6841 aatcgccctg cagttgaccc gcgatgccga atgccgcgac catcggccat gcacacattc
     6901 gcccaagccc tgttcggtct gcttttcctc gccatgccgg cgatcggcca cgcgatcgag
     6961 gaaccatcct atgaagtggt gcgcaagatc gacgacgtcg aagtccggca gtacgcgccg
     7021 tacatcgtgg ccgaggtaca gatcgaggcc gacgccgacg aagccggcaa cctcgcgttt
     7081 ccgatcctcg cgggctacat cttcggcaag aacaagggca agcaggaatt cgcgatgacc
     7141 gcaccggtga cacaggcagc cgctccagtg cgactcgaga tgacggcgcc ggtgacgcag
     7201 gcggctgcca gcggcacacc gggcagtcat gtcgtccagt tcgtgctgcc ccggggagtg
     7261 acgctgcaga cggcgcccga gccgctcgat gcgcgggtca ggcttcgcga gatgcctgct
     7321 cagcaggtgg cggtgatccg ctactcgggc ttctggtcgg acgccaacta caaagagcat
     7381 ctcgaaaagc tccgggcgac gctgaaggca gccgatctga cgccttcggg ggagccggtg
     7441 ttctccaggt acaacccgcc gttcacgcca tggttcatgc ggcgcaacga gatctggctc
     7501 gcacttcctt ccgtgcgctg acgcacagac cccggcggcg ggccgcacta tggtgaagcc
     7561 accttgacat tcaacgctcg caatccgacg acgggccaac catgatccgc atcgaactgc
     7621 acgtcgaact gagctacgag gtcgatgccc agggggctga cttcgtgttc aacatccacg
     7681 ccgcgaagac gcgctgccag acgatcgcgg ccgagagcct ggtgatcagc cagctcgttc
     7741 catcgcagat ccacaccgac ccgtcgacgg gcaaccggta tctgcgcctg cgcgcgctgc
     7801 ccggccagct gaagctgtcc tacatggcga cggtggacat cacccaccat gtcgccgatc
     7861 cggcgcagct cgcggaagtg ccggtgcacc tgctgccgcc cgatgtgatg ggctacctct
     7921 accccagccg ctactgccag tccgatcggc tggtgaagtt cgcgatcaac gagttcggaa
     7981 cgttgtggcg cgggcacagc cgcgtcgagg cgatccaggc gtgggtgcgc aggcacgtca
     8041 ccttcacgtc gaacacctcc aactccaaca cgtcggcggt cgacacgctg atcgagcagg
     8101 tcgggatctg ccgggacttc gcgcacgtga tgatcgcgct gtgccgcgcc ctgagcattc
     8161 cggcgcgctt cacgaccgcg accgactacg gtgccgaccc ggtcctcggc ccacccgact
     8221 tccatgcgta tgtcgaggtc tacctgggcg accgctggta cctgttcgac ccctccggga
     8281 ccaccatccc gatgggtctg gtgcgcttcg gcaccgggcg cgatgcggcc gacgtggcgt
     8341 tcgcgacgat cttcggcagc gtcgtgaccc acgcaccggt gatccacgcc tgggcagtcg
     8401 acgacccctc acgcggcatg acgatgccgt tccgctgcct cgaagcgctg tcgaccgacg
     8461 gcggcgcgcc gtcggcggcg tcgtaggccc gcgcgcgaca ccgatggcga tgttcgggct
     8521 ggagcacagc cggatggcct acggggccga cttcgcgttg cacggtatgg ccgtggcaac
     8581 cctggcagcc gcgctgatgc tggccggacc ggtcgatcgg tggatgccgc tcgcgtcgat
     8641 cgcattcgcc ggcctgctgg cctggaccgc cctcgaatac gccctgcatc ggttcgtgct
     8701 gcacggcgtg cgtccgttca gcgactggca cggacagcat cacctgagac ccgttgcgct
     8761 gatctgcgcg ccgacggtgc tgaccgcgct gctgttcgtc gtcggggtct tcctgccggc
     8821 cctggcgctg ttcgatgtgt ggatcgcctg cgcatggacg ctgggcctgt tgagcggtta
     8881 cctcgcctac gcggtcacgc accacgccac ccaccactgg cgggccgaca gctcgtggct
     8941 gaagcggcgc aagcgatggc atgcactgca ccaccaccac accgagcagc cggcgttcta
     9001 cggcgtgacc accgcgcagt gggaccacct gttcggcagc accggccagc cgcaccgcaa
     9061 ggaccgcgat ccggacggac gctggcggcg cgcctgaaca aatcccgcat ccggtcacaa
     9121 cgggcctcga cctaggactg gtcctacggc aggcagcgca gcgtccgaca gacagggccc
     9181 gctgcgcttc ctaccatcgg ttcagcgcgg taaacgacgt ctgcgagacc ctcagattcg
     9241 ccagccacac cgcatccctt cacagtttcc tgtgcgacct tccggccgtg caggtggagc
     9301 ctctccatgc acaccccatt gattgccgcg ccgcgctgga gtaccgcttc gttcggcgac
     9361 gccaccgaca cctcgccgat ggaactgtcc gcgctgggcg agcacctcga ccgctgtcac
     9421 gcctcgaaag gccgcctctt cgatgcgcgc tgtgccgcgg aggccatgca cggcttcgtg
     9481 gcatcgcgct tcgtcacgac cctggtggtc gcctcgctgc tgatcggcat cagctcgctg
     9541 attctctgag gcatggtgcc gtacgagttg cggcgcctga tccccgatgc agcgcccgcg
     9601 ctgtgcgcca tgctcaatgg cgcgcgcggc cggatcgaag caccgatccg atcggagatc
     9661 ttcggcctgc agcgtttcgc acagcacggc cgcagcctcg gtgagacaca ccgtgccgcg
     9721 ccggcgagcg tgcgcagcgc gccgttcttc ccgcgcctgc gcaacaacat ccagaccctg
     9781 cgtgaagccc acgcctacat aggcatccag gcgcggaccg gatacgacat cagtccggcg
     9841 gccgaatggc tgctggacaa cttccacctg atcgaggcgc agctcgagga gatccacgcg
     9901 ggtctgcccc ggcgctattt ccgcgcgctt ccagtgctgc tggacgaacc tctggccggc
     9961 ctgccgcgcg tctacggcgt cgcgtgggcc ttcgttgccc acacggatgg cgcattcgac
    10021 gaggagttgc tgatccagtt cctgcgtgcg taccaggaaa cgcgcgaact gaccctgggc
    10081 gagatgtggg cgctgccgac caccttgcgg gtggtgctgg tcgagaacct gcgccgactg
    10141 gccgagcggg tggccaccaa caaggcggca cgcgaggtcg ccaacctgtg ctgcgaccgg
    10201 atcgacagtg cctcggtcga cgcgctcgac gacctgctgg ccttgatggc gcgacgcggg
    10261 gtggagcggg tgttcctcgg acagatgtcc ctgcggatgc aggacggccg gaccacggcg
    10321 caaggtggca accgtgccag cgaccacgcc gacgcgcgcg ctgcgcagca cgaatggctg
    10381 aggcgcgcgc tgccggaccc ggcggcgatc caggcgcagc atcgcgccga ccagaccgcc
    10441 gacaacctca gcgtcagcaa cgccgtgaca tcgctgcgtg ccatcggcga tgccgactgg
    10501 accgacatcg tcgcccgcag cagcacgctg atgcacctga tgctcagctc gccggtattc
    10561 gaggccgaac acgcggtgac gcgtgaccgg acgctgcatg gcatcgaacg gctggcgcga
    10621 aagagcggcc gcagcgaagt cgcggtggcc cggaccctgc tcgacctgat gcaaccggcg
    10681 cagggcgaag ccgtcgcact cggcggtgaa acgagtaccg ccgtggccag ccactggctg
    10741 agtggtgcag ggcgccccgc actgatgcac gcgctcggtc tgagcgagcg cgcagcagcc
    10801 tcgtggcgag ccgtggcgca ccgactggca ctgccggcct acctgggctc actgttgttc
    10861 gtcacgctgg ggctggtggc ctggctgctg cgccacggcg gcgggttcga cgtgtccacc
    10921 gggctgatgt ggctggctgc tgcgctgatg ctttttccgg cctcggaggc cgtggtggca
    10981 gtgatcaacc ggctgatcag cgagtcagcc cggcctgcgc acctgccgcg tctggcgctg
    11041 actgcgggca tcccggccga gcaccgggtg atggtggtga tcccggccat gctgaccgac
    11101 acggcctcga cccgggccct ggtgcatcgc ctgcaactgc atcacctggc caaccccgag
    11161 cgccatgccc agttcgcgct gctgaccgat tgggcggatg ccggcagcgc gcgggtcggc
    11221 acggacgacg ctctgctggc agaggcgcag cagatgatcg aagcgctcaa cacgcgccac
    11281 ccgacctgct accaggctgg gcgcgacgag ctgacggcca gaaccgcggc tccgccacga
    11341 ttcatcctgc tgcaccgtga acgtcgctac agcgagaccg agcagcaatg gataggctgg
    11401 gagcgaaagc ggggcaagct ggagcagctg atcacgctgc tggccgaggc ttccgatcgg
    11461 ccatcgacag acgccctgcc cgcgttcatc gatctcggcg aggcctcgag gatcgcgctg
    11521 gacaccaagt acgttgtcac gctcgacagc gacacccagc tgcccccggg ccgcctgcac
    11581 gacctggtcg gcgtcgccgc gcacccgcag aaccagcccc gcatcgacgc gacagggcgc
    11641 gcggtcgcga gcggctacgc gatcctgcag ccgcgcatcg tcacgccgct gccgtcgccc
    11701 aaggatgtca cgctgtacca ctggctgttc gccggccagt gcgggatcga cccctacagt
    11761 gccgccacgt ccgaggtcta ccaggacgtc ttcggcgagg gcacgtacac cggcaagggg
    11821 ctgttgcacg tccaggccat gcatgcggtg ctgtcgggtc gcctgccgga aggccgtgtg
    11881 ctgagccacg acctgctcga aggttcgatc gcccgctgcg ctgcagtcac cgacatcacg
    11941 ctgatcgagg actcgccgtt ccatgccgac gtggccgcgt cgcgcgtgcg ccgctggacg
    12001 cgcggcgact ggcagctgct gccgttcctg ctcgattcgc tttcgaccga ctcccggtac
    12061 cgcctgcgag ccatcaaccg atggaagatg ttcgacaacc tgcgccgctc tctggtcgcg
    12121 ccgatgtcgc tcgcgctgct gctgctggcg ctgggcggct cggtgatgtc gccctgggcg
    12181 gcgcttgcac tggtgctggc ggccttctcg gccggacccc tgatgggggc ggtcgctggc
    12241 ttctccccaa gccgggacga catcgcgcag cgccacttct acagtcaggc cgccagcgat
    12301 ctggcccgag cgctgtgcgg cggagcatgg ctgctggtcc agttgcctgc gcaatcgatg
    12361 caggcggtgg atgccatcgc gcgcgccgtg caccggatgg ctgtcagcgg gaagcacctg
    12421 ctgcaatgga cgaccgcagc ggccgcacag gctcgagccc ggaccgcctt gccggcgctg
    12481 ctgcgccagc actggcgcga gccggtgctg gccttggcgc ttctggccgc gctggtcgcc
    12541 gtcggcacgc cgaccctgtg gctcgccatc ggcctgtgcc tgtcgtgggc tgccgcgccc
    12601 gtctggacct ggtgggtcag ccggcctcgc ccggcctgtg ccgaggaagc cctgtcggcc
    12661 gaagaccagc gctacttcga ggtggtcgca cgcgacacct ggcgcctgtt cgagcggtgc
    12721 gtggtcgccg cggacaacca cctgccgccc gacaacctgc agaccctgcc ctacgacatc
    12781 gtcgcccatc gcacgtcgcc gaccaacatc gggctgtacc tgctgagtac cgcctgcgcg
    12841 cggcagttcg gatggatcga cacagccgag ctgatcacgc ggatggaggc cacgctcgcg
    12901 acgatgtcgc gcctgcagcg ccatcgcggg cacttcctga actggtacga cacgcagacg
    12961 ctcgcaccgc tgctgccgat gtacgtgtcc accgtggaca gcggcaacct cagcgggcac
    13021 ctgctcgcgg tcgcgcaggc gtgcctggaa tgggcgcgcg acccgcacca cagaccgaca
    13081 ccacagaccg aagcacgcct gcaggcgcta gccgagcagt gcgaggccat cgcatggcaa
    13141 gccgatttca gcttcctgta ccaccacaag cgccacctgc tgcacatcgg ctaccgggtt
    13201 gccgagcagc agctcgacgc gggcttctac gatctgctgg cgtccgagtc gcggctgacc
    13261 agcctgctgg cgattgccaa gggcgacgtt ccggtgcgcc actggtcgtc gctcggccgc
    13321 atgttctatg cggtcggttc cgaagcggga ctgcgctcgt ggtcggggtc gatgttcgag
    13381 tacctgatgc cgacgctgat actcgacgag cctcacggca gcgtgttgca cggggcctgc
    13441 cacgccgcct tgcgtgaaca gatcgcattc gccctggtcc agagcgtgcc ggggcgttcg
    13501 gtcccgtggg gcatctcgga gtcggcctat gccggccagg atcacacgct ggcctaccag
    13561 tattcaccac agggggtgcc gcggctcgca ctgcggcgaa cgccgccaga cgaactggtc
    13621 gtcgcacctt atgccaccgc gctggcagct cagatcgctc cgcaccttgc ccgactcaat
    13681 ttcgcggcac tcgaaacgct ggcgccgcgc gatcactacg gattcatcga agcactcgat
    13741 ttcaccgcat cccgcaagac cggcgaggaa gcattcatcc cggtcagcac cttcatggcc
    13801 catcaccagg gcatgaccat cgtcgcgctg gccaacgtgc tgctgggtgg ggtcgcgcag
    13861 cgctggggca tggccaatgc gcacatcgag gcgatggcct cgctgctgca cgagcgggcg
    13921 ccgcgcgagg tgtcgatgct gtacgcaccg cctgcagcac caccgccact ggcgatgcag
    13981 cgccgcgccc ccggcttgct gcgcgagctc ttccccggtg cgaccgcgct ggagccgacc
    14041 catgtgcttt cgaacggccg ctacagcgtc agtctgcgcg ccaacggctc cggatggagc
    14101 cgctggggca ccacgggcat cacccgcagc cgcgacgacg cactgcgtga tgcccagggc
    14161 agcttcttct acctgcgtcg caccggtgcc gagcacgctg gcgaagcgac cccggcggca
    14221 tcaccgctct tctccatcac ccagcacccc gcccccgatc cggcggccca ctaccgcagc
    14281 atgttccacg cagatcgggt gtgcttcgac gccgcctggt ccgaagtgca ggcgcacacc
    14341 acggtgtggg tcagtccgga ggacgacatc gagttccgcc agatcgaact gcgcaacctc
    14401 ggcgacgacg tcctcgacat cgaactgatc tcggccttcg acgtgacgct ggccgatccg
    14461 cgcgcagacg aggcccaccc cgcgttcagc aacctgttcg tgcgcgccga gtggcagggc
    14521 gagcagcagg cgctgctgtt cgagcgcacc ccgcgactgg ccaccgagcg cgggttgcag
    14581 gccgcgcatt tcctggccga caccgacccg caggtggttt cggtgcggct gcagaccgac
    14641 cgccagcact ggctggggcg caatcgcagc acccaccagc cgcaggcgtc gttcgagcac
    14701 cttcccgccg acgacattgg cacgcaggcg gcgctggaca ccgggctgga cccggtctgc
    14761 gcgatggccg tacgactgcg gatcgaaccg cagggcaagg ccatgctgac cttcgcgacg
    14821 acggcctccg acaacggcgg cacgctgcgc gcggtgatcg acaagtaccg gcagaccagt
    14881 cacgtgaagc gggcatcgct gatgtcggcc acgctggcgg gcatccgatt ccgcacgctg
    14941 cgcatcagca ccgagaactt cgatgcgatc cagacgctga ccaccgcgct ggtgctgacg
    15001 ctgagccgcc cacgtgcgcg cgcggcatcg ctgaacgacc tggctgccgg ggtctgcgac
    15061 cggcgcctga tctggcgctt cggcatctcg ggcgaccggc cgatcctgct ggtgtctgcc
    15121 ggcgcgccgg agggactcgg cctgatccgt tccctggcgc aggcgcttcg cctgtggtca
    15181 tggagcggca tcgcctgcga tctggtggtg gtgaacgccg aaccggcgtc gtacctgatg
    15241 gccgtgcagc gcgagctctc ggcgatgcgc gagcgccacg gcgccgactc gggcgccgag
    15301 ttccggcctg ccggggcgac gggcccttcg agtaccggcc tgcacgtgct gcgggcggac
    15361 gagatctcgg ccgatgaact gagcaccctg caaagcctgg cgcgccttcg gctgcaggcc
    15421 gacgggcggc cgttgacgca ccacgtgcag gaatgggtgc aactgcacga agaggccttc
    15481 gagcagcgtc acgacacctc cacggtgccg gtatcggtgt cagatggcgt gggaccggcc
    15541 gtgccagccg ccgccggttc tttcgcggcc tcgtcgggtg agtttcgttt cgccgtgagc
    15601 gcccggtctc gcccgatgcg cccctgggcc aacgtgctgg ccaatccctc cttcggtacg
    15661 cagctgaccg aggccggggc gggctacacc tgggcgttga acagccgcat gaaccagctc
    15721 acggcctggt cgaacgaccc ggtgggcgat ccaccgtccg agtggttcct gctgcaggac
    15781 ctgaagacgc tggacgcctg gagcatcgcg ccctcggcct ggggcgacga cagcgtcacc
    15841 taccgcatcg cgcacgggca gggctacagc gtgatctccc accgccgcgg cgacgtcgag
    15901 gtgacggcat cgtggtgcgt ggatccgcag agctcggtca agcaggttcg cctgcgcgtg
    15961 ctgaaccgcg gccaccggac cctgcagctg cgcatggtcg ggatcgccga atggatgatg
    16021 ggtgccaacc gttcggaccg cgccacggcg cacaccgcgc tgcatcgcca gcgtctgccc
    16081 gacctgaatg cgccgaacgg aagaagcgat ccgggccgct cgcagaagct caccgcgctg
    16141 atgtgcaccc agcacgagcg ctcggccgga ttcggcgacg gcacggcgtt cctggcgatc
    16201 gccggcccgg acgagcgcga cgacaagctg gagaacggtg gcgactggac ctgcgaccgc
    16261 cgcgagtgct tcgacgcgcg cggccgactg gtgctgcccg atcatttcgg tcagcaaggc
    16321 ggcagcggtc tggatccctg cgcggcgttg tcgacgaggg tgaggctgct cgccggggaa
    16381 tccgcagaac gggtgttcct gctcggctac gccgacagtc cggacgcagc gcgccagctc
    16441 gccgcacggg cggcaaccgt gcctgcaggg cagcggctgg aagccgtgcg cgagacctgg
    16501 gatgcgctgc tcgaggccac cacggtgcgg acgcccgatc cgctgttcga cgccctggtc
    16561 aatcgctggc tgctgtacca gaccgtgtcg tgcaggctct gggcccgtgc cggcttctac
    16621 caggcgggcg gggcatacgg cttccgggat cagctgcagg acgcgatggc cctgacctgg
    16681 gccgcccccg gcatgctgcg cgaccagatc ctgctcggag cgtcgcggca gtttgcccaa
    16741 ggcgatgtcc agcactggtg gcatgcaccg ggcggagccg gtgtgcgcac gcatttctcg
    16801 gacgacctgc tgtggctgcc gcacgcctgc gtgcgctacc tgcgcaccac cggtgacacc
    16861 ggcgtgctcg acgagagcat cgcgttcatc gagggcgcac cgatccccga cggcgccgag
    16921 gatgcctact acacgccctc ggtcagcacg gagcaggcct cggtctacga gcacgcggcg
    16981 cgcgccattg atcgcagcct gcgcgtcggc gcgcacggcc tgcccctgat gggcaccggc
    17041 gactggaacg acggcatgaa ccgtgtcggc cacgaaggcc gcggcgagtc ggtgtggctg
    17101 ggctggttcc tgtgcgagct ggtcgacgat ttcgcaccgc tggcgcgcga gcgcggcgat
    17161 gacgcgcgcg ccaaggcctg ggaagccgcg gcgctcggct ggcgaaacgc gctgaatggc
    17221 atcgcatggg acggacagtg gttcaagcgc gcgttctttg acgacggaca ggcgctgggc
    17281 tcgcacgtca acccggaagc gagcatcgac ctgatcgcgc aggcctgggc ggtgctgtcc
    17341 ggggccgcgt cgccagagat gcagcgcgag gccatgcatt cggtcgaggc ccgtctggtg
    17401 gaccacgagg ccgggctgat caagctgctc gacccaccac tggcccatgc gcagccgagc
    17461 gcgggctaca tccaggccta tccgccggga gtgcgcgaga acggcgggca gtactcgcac
    17521 gccggggtct gggcgctgat ggcgcaggcg cggttgcaga ccgacggcgc cgacaacggc
    17581 gcgcgcagca gtccgccgga catcgacctg ccttaccgct atttcagctt cctgagcccg
    17641 gcgcaccgtg ctgcccaccc gacacgcgga gcggcctacg gccttgagcc ctacgtgatg
    17701 gccggcgacg tctacacgca ggcgccttac gtcgggcgcg gcggctggag ctggtacacc
    17761 ggggcggccg cctggctgca tcgttcggcg attgaatcga tattcggcct gcagcaagga
    17821 gcccggacgc tgagtttcac gccgtgcctg ccatcccact ggccgcaggc cgagctgacc
    17881 ctgaaacgag gccagggcca ggcgtgccgg acgatgcgat tcatcctggt ccggccggcc
    17941 gcactcgccg agctggaagc caccgcgccg tggggcgcgc agattctgct gcccggcgag
    18001 tcactggcgt ggcaagacct ggcgccgcat gcgtgcttcg tcattccgct gcccgcactg
    18061 acaacgaagg aattgccggg tgatctgccg gcgtcagccg accgcatcgc ggccccggcg
    18121 atctagactc gacgctggtt gtccgccgca ttcgacgccg acacacacgc tcctggcatg
    18181 caagcaccca ccgactcccc catcccgcgc cattcgcggg cagatggacc gccactgatc
    18241 ttcacgccgc gagtcctggc aacgccgtga atccacccgc cgttcgtctc gggcacggtc
    18301 gcgtgctggg tgtgctgctg ctgatggcgc tagcgtacgc ggtggtcgga cgggtgtcgc
    18361 tgctgctggc cattcctccg gcctatgcct ccccgctgta cccgcctgcg ggcatggcgc
    18421 tggtgttcgt gctgctctac ggacgcagcg cagccgcagc ggtggcggtc ggggccatcg
    18481 tcatcaacct gctgctgaac ccattgcgcg cggtcggcct cgccgaggtc gtgatcggtc
    18541 tgggcgccgg actccaggcg ctggtcgcgg cagaactggt gcagcgcatg gtgcggcaac
    18601 cgttgacgct gtcggcgccg cgcgacatcg ctcgcttcta tctgtttggt gcgctgatcg
    18661 gatgcctggt cagcgcgagc gtggcgactg ccgcgctggc cgtggcggac gtcgtgcccg
    18721 cgtccgcctg gccgatgacc tggctcacct ggtggatggg cgatgcactc ggcacgctga
    18781 tcgccgcgcc gatcctgctg acgctggcgg gacggccccg cgaggcctgg gcgccgcgcc
    18841 gacgcaccgt cggactgacg ctgggcatcg tgaccgccct gctggtggcg accaccgtgc
    18901 tggtggccca ctgggatcgc gagcgcgtcc agaccagctt cgagcgcgat gcagcggcgg
    18961 catcgatggc cgcccaggcc cagctgaaca ccccgctgca tgcgctggag gcactgcgcg
    19021 ggctctacat cggatcgaac gaggtcaacc ccggcgagtt gcgcgctgcg gccgcctact
    19081 ggctgaatcg cgacggggac ctgcaggccg tcggctatca ggagcgcgtc cggctggtcg
    19141 acgtgccggc gctcgaggcc cagttgcgtg acgccgggtt ccccgattac cgcgtgctcg
    19201 atcgcacggc cacggacgaa cgggtaccga ccacgatgcc cgaggcgtac gccgttcgac
    19261 tcgtcgagcc cagggaaggc aacgtcggcc tgctgggcat caacacgttg tcgatcccgg
    19321 cagcacggca ggcgatcgag gcctcggtgc gcaccgaccg accggtggcc agcgcctcct
    19381 tccacctgtc gcaggacgcg gtgggctcgc cgaagacagg ggtggtgatc taccgcgcga
    19441 tctaccgcgg ccagcccgcc accgaagagg agcgggctgc ggcagtacgt ggcgtggtgt
    19501 tcgtcaccgt gcgcgtggag gacctgctgc gctcggtgct ggcccaggcc cccgcctacc
    19561 tgcacttctg cgtgaccgac acggacacat cggcccgcct gcgacagctg gcggccacct
    19621 cgctgtgtgc cgaggcaccc gacgcgctgg tacaccggca ggtgattgcc ttcgcggggc
    19681 gccaatggga atggcgcgtc aacgccagcg aggtcggcgc cagtcaggca gccggtgccc
    19741 gccactatgc ctggctgttc tcgagtgtcg gcctgatgac ggcagcggtc ctgggagccc
    19801 tgctgctgac ggtgaccggc cgcgcccgca tcatcgaact ggccgtgcag gaccgcaccg
    19861 ccgcgctgga gcagcaggtg cgcgagcgcg cgcaggtcga ggccgcgatg cgcgaaagcg
    19921 agcagcgctt ccgcaacatc ttcaacaacg tgccgatcgg tgtgctctac accgacatcg
    19981 acgggaaggt gcgacaggcg aacccgcgct tttgcgaact ggtcggacgc gacgccgcca
    20041 cgctgtcccg tctcagcacg cgggacttcc tgcatcccga tgacgtcgac gaggacgcgg
    20101 ccctgttcgc ccggctgctg gccggggaaa tcgcgatcta caggcgacag acgcgctatg
    20161 tcgcacccga tggtcacgtg gtgtgggtgc agcgcagcgt gtccctgctg cgcgacgaga
    20221 cggggcgccc gcagcgcatc gtcagcgtgg ccgaggacat caccgagacc ctgcgcctgg
    20281 cggaggcggt gcgcgcccga gaggcggccg aggcgtcgaa tcgtgcgaag agcgagttcc
    20341 tgtcgcgcat gagccatgag ctgcgcacac cgctgaacgc catgctcggc ttcgcccagt
    20401 tgctggaact ggaggatcga ccggcgcttg gccgcgccca gcgcgcctgg gtcgggcaga
    20461 tacagcaggc cggctggcac ctgctggaga tgatcaacga cgtgctggac ctgtctcgca
    20521 tcgaatccgg caacctgcgc atggagctgg aaaccctgga cctggccgag ctgctggcgg
    20581 ccacgcgtcc gctggtgcag cagagcgcag acctgcgcaa catcgtgatc actgaggatc
    20641 tggcgcgcgg cgcgaacttc gtcaccggcg atgccacgcg ggtcaagcag atcctcacca
    20701 acctgctgac gaacgccgtg aagtacaacg tcgacggcgg gcgcatccag ctgtccaccc
    20761 gggtgcaggg cgactgggtc gagttgatgg tcaccgacac cgggctgggc atgagcagcg
    20821 agcagatggc ttcgctgttc cagcccttca accggctggg ccgcgaactc accggaaccg
    20881 aaggcaccgg catcggcctg gtgatcagcc agcggctggc cgagctgatg ggcggcgagc
    20941 tgaccgccgc cagccacgtg ggcagggggt cgtcgttcat gctgcgcctg ccggccgcag
    21001 ggaacccggg cacggtgcca tccgacctgg acggcctggc cgccgatcgc aacgattacc
    21061 agcagcgcca cgtccattac gtcgaggaca acgaaacgaa cgtggaagtc atgcggggca
    21121 tcctggcgca gcggccccag gtgcagctcg acgtttcggt caccggtctc gacggcctgg
    21181 cggcgatccg cgcccggcgc ccggacgtgg tgctgctcga catgcacctg cccgacatca
    21241 gcggactcga gctgctgaga cacctcaagc tggatccggg cacccgttcg atcccggtgg
    21301 tcgcggtgtc ggccgacgcc ctgccggcgc agatcgacga cgcgttcatg gccggagcgc
    21361 accgttacct gaccaagccg gtcaatgtct ccgaactgct gtcggttctg gacgaattgc
    21421 tgagtcaggc agacaccggt ttcgagtgac acgcctttgg cactcaatga acgagagtgc
    21481 taacatcatg gaaccaagcc tcgtttgagg gagtccatga ctgccatgaa catgaccatt
    21541 tccaacgctt ccaatacctc ggccctggcc ctgcgggatc cgtgggccat ggtgccttcg
    21601 ctgggcagcc tcgacgccta catctcggcg gccaaccgcg tgccgatgct gacgctcgag
    21661 gaagaacagt ccctggcccg ccagttgcgc gacaagggcg acctgcaggc ggccggtcag
    21721 ctggtgctgt cgcacatgcg gctggtggtg tcgatcgccc gccagtacct gggctacggc
    21781 ctgcctcacg gcgacctgat ccaggaaggc aacgtcggac tgatgaaggc ggtcaagcgc
    21841 ttcgaccccg atcagggcgt gcgcctggtc agctatgcaa tgcactggat caaggcagag
    21901 atccacgagt acatcctgaa gaactggcgc atggtcaagg tggcgaccac caaggcgcag
    21961 cgcaagctgt tcttcaacct gcgctcgatg aagcagagcc tgaaggacga tgctgtcgcg
    22021 ggcgagaccc accgcagcac gctgacgcag ggcgaggtcg acacgatggc gcgcacgctc
    22081 aacgtcaagc gcgaagaagt gctcgagatg gaaacccgcc tgtccggcgg cgacgtgccg
    22141 ctcgagccgc tgaccgacga cggcgaggaa agctacgcgc cgatcgccta cttggccgac
    22201 gacacacacg agccgagcca ggtgctggaa gcccagcgcc gggactggct gtccagcgac
    22261 ggcatctcgc tcgcgctcga cagcctcgat gcgcgcagcc gccgcatcgt cgaagagcgc
    22321 tggttgaagg tcaatgacga caactcgggc ggcatgacac tgcatgatct ggccgacgaa
    22381 tacggtgtca gcgccgagcg catccgccag atcgaggtcg cagccatgaa gaagatgcgc
    22441 aaggctttgg gcgcatcgca ctgatacgca gggcgcaacc gtaaaaaacg gccccgaggg
    22501 gccgtttttc atgggcgcgc caaaggcctg cggtcaaccc agcgccagca gcgtcttcag
    22561 gtcggcggcc agggcctgcg agccgctgcc gtagcggatg aagagccgca ccttgccctg
    22621 ggcatcgaac acgtaggagc ccgccgtgtg atcgacactg tagctgccct cggtcttgcc
    22681 gggcaccttg gcgtagaaca ccttgaattc cttggccgtg gccgcggtct gttcgggcgt
    22741 tccgcgcagc gcgacgaagc tggggtcgaa gccactcatg tagcccttca gcacttcggc
    22801 ggtgtcacgg tcggggtcga ccgtgacgaa gacgccctgg atacggtcac cgtccttgcc
    22861 gagcgcagcc ttcacctgcg ccagctcggc catcgtcgtc gggcacacat cggggcactg
    22921 ggtgaaaccg aagaagacca cggtgacctt gcccttgaaa tccgccaggg ttcgggcctg
    22981 cccgttctga tcggtcaacg acagcgagcg cgcgtattcg gcgccggtga tgtcgatgcc
    23041 cttgaacgcg atcgcagtgc cgccagacga tggcgcatcg cagccgagca gtgccagcgt
    23101 cggcagcgat gccagcgcca gcgtggtgcc ggccctcagg aagcggcggc gagcggaacc
    23161 ctgttcgaac agcagatcga tcacagcaga gggtccaggt aatggtcgat cagcagtgcc
    23221 gcgaacaaca gcatcaggtg ccagatcgag aagcgaaacg tcttgcgcgc cagcgcatcg
    23281 gaatagttgc gccagagctt ccaggcgtag ccggtgaagc ccgcgccaag cgcaattgcc
    23341 gagaccaggt agatccatcc gctcatcccg gagatgaacg ggagcagggt cgcggcgaac
    23401 agcaccagcg tgtacaggaa cacatgcagc tgggtgaagg ccgcaccgtg cgtgatcggc
    23461 agcatcggca ggccggcctt gcggtagtcc tcggcgcggt acagcgccag ggcccagaaa
    23521 tgcggcggag tccacaggaa gatgatcagg cacaggatca gcgattcggc gccgacctcg
    23581 ccgcggatgg ccgcccatcc cagcaccgga ggcatcgcgc cggatgcccc gccgatcacg
    23641 atgttctgcg gcgtcgcagg cttgagcaga acggtgtaga tgaccgcata gcccacgaag
    23701 gtggcgaagg tcagccacat cgtcagcgga ttgaccatcg tgtacagcag cacgctgccg
    23761 atcgagcaaa gcaccgccga aaacgtcagc gtctgcgcgt tcgtcagttc gcccttggcg
    23821 gtcgggcgcc aggaggtgcg agccatcttg gcgtcgatgt gctgctcgac gacgcagttg
    23881 aacgcggcgg cggccccagc gaccagccag atccctgccg ttgctgccaa cgccagcttg
    23941 atgtctggca gcccgggcac agccagcaac atgccgatga cggcgcagaa cacgatcaac
    24001 tgcactacac gcggcttggt cagcgcgtag aactgcttca gccgcgacga gcgtcgggcc
    24061 aggggcagcg agggggcaag tgtttcggac atgttgaaca gcctgcgatt ctacgaagcc
    24121 gctccgaggg cagctgaagg aggcactgcc tgagcagggg acaaggggtg cgcccgatac
    24181 gcccggctga gcatcgtcgt cagcaccacc accagcgccg cggcgccgcc ggtgtgcgcg
    24241 acggctgcca caagtggcca gtccagcacg acgttgctca gtccactgat caattgccag
    24301 gcgacgacca ccgccagcac gccagcccac cgacgcagct cgggctggcc cgaagcccgc
    24361 aggcgccagg ccagcgctcc gatggcaacc accaccacgg ccgcgccgag acggtgtgcc
    24421 atgtggatcg ccgtcagcgc cgcgaacggc aggtagccgc cgtccttgcc ggcacccagc
    24481 tcacgcgcca cggtgaaacc gtgctcgaag tccatcggcg gccaccatgc gccctggcag
    24541 gtcgggaagt cggtgcaggc caggaccgcg tagttggtgc tgacccagcc cccaagcgcg
    24601 atctgggcca cgctgaccaa gaacaccagc accgtacccc ggaacatcgc gcgggacatc
    24661 ggcagcggtg gctgctgcgc ctggctctgg acggacaaca gcgcgagcaa gcccagccct
    24721 ccgagcagat gcagggtgac gatggccggg taaagcttca tcgtgaccgt cagcgcaccg
    24781 aaagctccct gcccgcagac ccacacccag gtcagcgtgg cccaccaggg agacaacctt
    24841 gccggcagcc cgtggcggaa cttgcgcgca tcgagccacg ccgccacggc caaccaggtg
    24901 atcagcgcgc ccacgcccgt ggccaggtag cggtggatca tctcgatcca ggccttgcca
    24961 tgcgtgaccg ggccggtggg catcgcggtc tgcgccgcgc cgatggcctg gagcgcaccc
    25021 agcgggctgg cactgccata gcagcccggc caatccggac acccgagccc cgaatcggtg
    25081 agccgggtga aggcaccgaa cagcaccagg tcgaaggtca ggaacagggt gatgagcgtc
    25141 agcgcctgcc gccgacgcgc cgcatcggcc cggcggtacc gccatgccac ccacaacacc
    25201 ggcgccagcg ccagcacggc accgacgaga accagcgtgg gtacgggacc caggtcgtag
    25261 gctggcggat tcatgcgatg aaactcatct ggaagaaatc gttcgacgag caggctgcag
    25321 gtcagcgacc tggctggtcc caggaggccg acgcgcgcag caggcgctcg aggtccttct
    25381 tcagcttggc cggttcggga ccgaccggcg tacgcatcat ccactgacct ttcggatcga
    25441 ccaggtacac gtgttcgtcg agcctgtgtc cgtccgacgg cttcagccac tgggacaagg
    25501 cctcgccagg cacctgcagc acgtggctgg cccgacccgg tgcgatcgcc ttcagcaatt
    25561 cggcgcgcgg cgctgcctca ccggggatca accagacctt gtcgacgcga ggcttgtcgt
    25621 tgcccagcat ctcctgcacc tgacgctgga gcagcagatg ggactggcat cctgcgtcgc
    25681 aggccccatc ggccaccacg acgatgagcc actgcccgcg caagctggca gccagcaccg
    25741 gctgcccggc caggttgcgc agcggcaggg tctcgggcat ctcgcgcgtc ggtgtgacga
    25801 actcgctgta attggtggtt cgaccttcga gcccgatcac gaaatagatg atgtaggcgg
    25861 cgatcaccgg cgcagagcag atggccaata ccgccagcat cttcaggcgc ccggccgcgg
    25921 tgcgccgagc agacagatcg ggcgtcggca tcgaatgcac cgtgaagctc agcggcgata
    25981 cctgctcggg cgcatcgctg cgatcggacc caagggtggg ggagttggaa ccagacatac
    26041 agacctgtca tcaaggcgca atgcgcaaac cactggaacg cgtgaccgca gtgccttggc
    26101 atcccgccag aggcggttcc gccgacgggg caaaccgacc cgaggcgcac catcggcgtt
    26161 ctgagcggca gacagcggca tccgggacac cgaggacatt ctttcaaaca cacgtcgagg
    26221 tcgagattct ggcggatgac actcgaggcc ggctgcgacg atgtcgaaca gccgccccgg
    26281 cgtcggggcg atgtgcccga gaaccgtgcg gcgctggaag cttggtgcca tcgagccacc
    26341 aggagcccgg tcttgcaacg gacgtcacgc cggtcagggc agcgacccgc acgacgagcg
    26401 cttgtagacg caaacggcgg gaaatctcat ggatcgcgac atcggcgcgg cctttcaatg
    26461 cacaggccat cggcacacgg ataatccgaa gccatgaaat acctcgtagg gctcgcattc
    26521 gtcaccatca tcggcgcgtt ggcctacgcc ggggtgtcca tggttcgcga cggtcgggat
    26581 ggcaaaccga agacccactc gatggtgaac gcgctggcat ttcgcgtggc cctgtccatc
    26641 ctgctgttcg tgtgcctgct cggaagctac aagatgggct ggatccagcc gacaggtttc
    26701 ctgcgacgct gagccccagg ttcagtcgct tccggcagca cgaacccgca gccctggacg
    26761 aagcgaaaag tcagaccccg ccacaccgcg acccacaggg tcgggtacgt ctctcaggga
    26821 tgagcctgct ggagcagcct gatcgggcgc acgccctgtc cgaaggtcag gatattcgca
    26881 gcgcagtggg ctgcagagat cttcttgccg cgcgcagtca gatcggcttg tttccccgcc
    26941 ctgccgggtt catcggccgg tgctgacatc tcgctgcaca ggcacaccac ccgcttcgcg
    27001 tacctggccg gcgacagcac cttcctgttg ggccttgatc gtcaggtcgg cggaactggt
    27061 gtccttcatg gcgacaacca ccttcgtcgc gggaaatgcc atcccgatga cgatgatgaa
    27121 cggcacgatg gtccagatga tctcgacggt ggtgctttcg tgaaaattgg cggccttcgc
    27181 tcccttcgcc ttgcggtgca tcaggaccga ccagaacatg aagccgaaca caccgacgaa
    27241 ggtcaccatg cagaagacca gcagggaata gtgcagccaa cgctgatctt cagcgatctt
    27301 cgtcgccgcg acgggcaggt cattgaccgc agcagccagc gtcgagacag cacacaggcc
    27361 gaggccggtg agggcttgtc cacctttgtt gcaaagtgcg ttcatcttgt tcatcgtgac
    27421 tgctcgcgtt gtccagactc tgccgcaagc agagcatcct gaggccgggt gcgggccagc
    27481 cgaaccgctt gtcgcaattc gttggccaac tgtcgccgca tctccgggcg cacaaaccgc
    27541 ccgacctcga tccttcgtcc ttgcccggac aactcgatca gcgactgatc accggtgacc
    27601 ggttccacgc tgacccactc ggtcgcaaac tcggccttct cgaccatgct gcccgaggtg
    27661 agttccaccg tgagccgacc gctgcgcatc gcgatgtttt cctgatcagt ggcatgccgc
    27721 gaatacacca tcaaggcgat gccgaccgcc gccagttcga tccacgcgaa cggcatgatc
    27781 agcttggcgc cctgcagcca gaaaaacccc gcgatcatca gcgacagcac gcaaaaacca
    27841 ccgaacacgg ccagcaattg tctcggcgtc agggagcaat tacgtttcag tatccattcg
    27901 acagaccact ccccggcacc cgaaccgctc tcgacgccga aatgccacgg cgcgtgcttc
    27961 gcgcgaacgc ttggtgcaaa aagtgatgga taagtagaca tccagcggca gcgggttcgg
    28021 acgagccaga gcggcactct ggcgcgtggg ttcgacttga ctcgaatcaa gggcaacgcc
    28081 ggattcgagc ctggaatgaa gcggacggca gtctactcga gtcgggaatt cctgtttcag
    28141 gccggtcgac gcgcctcacg catcaggcgc cgcatctcct cgagcgaaac ctgcgtctcc
    28201 gccgcaacct ggacaggtga atcggccttg aacgcgtggc cgtagatgat ctcgaaggtc
    28261 agcccgagtc gtccgttctg gtctcgcatg ctgtcaagtt cgcggtacag acgcgtcagc
    28321 cagcgcggtg tgcgcagccc cccgaaacgc cccggcgatg cgttgccgcc cagcaatcgc
    28381 aggtccttgc aaagcgcctg agcactctcc caatgcaccg tcaacgtctc ctgatccata
    28441 accgggtcgg cgaaacctgc ttgcagcagc atgtccccca ggtcgtgcat gtcaacgaaa
    28501 tcgacggtgg gtgccggcca tccctgcccg gcataaaggg ccctgagctc gcgcgcggtg
    28561 tccggcccca gacacgaaaa catcacgaat ccgccgccag ccagcatgcg ttgccaacgt
    28621 tgcagcaaag cgggcgggtc cttgacggca tgcaatgcca tgttggccca caacagctgc
    28681 gcacccgtgg gctgcacttc gttcgagtcg acgaccacct cgaccggcgg agccctccat
    28741 cggcgaccgc tccaccacgg cgatcgcaac gcctgccggc tgcgcagcgc cagatcctcg
    28801 ctgggttcga cggcgcgcag cgcagcctgg ggataggccg cggtcagcaa cgcactgccg
    28861 gcaccgagaa acgcacccca gtcgatcatc cgatccggcg acatgcggaa caaggccaag
    28921 cgctcggcca tgcgccctgc gatttcggca tgcagccaag gcggtgttgg caatcgaccg
    28981 agtcgacgct gggtggcggc gacggccgcc tcatcgatga ggcgctgcgt cgtgtgggaa
    29041 gctacagcca tcgccccagt atattgacgg cgtgaatgtg cagccccgtt tgatagaccc
    29101 tgatgaacga ccctggtggc gtcgcttgcc gggcctgtgc gcggtctgtc atggctgggg
    29161 ccgtggtcgg gtgtgcggca cctgcatcag ccgtttcgcc ccccacaacg atcgttgcct
    29221 gcggtgcgca gcaacgctga ccgccagggt cgaactctgc ggagactgca tccggaaccc
    29281 gcccccgttc gacagcgcgc tcgcagccgt cgactacggc cacccctgga accggctcat
    29341 ccgggaattc aagttcaacg attcgcccga ccttgccggg ccgctggccg aactgctggt
    29401 tgctgcacac cgtcggcgca ctcatgcgcc tccggatctc ctgctgccgg tgccgctggg
    29461 ttccggacgg ctgaaggagc gggggtacaa ccaatcatgg gagattgcgc gtcgatgcgc
    29521 ccatcggctg ggttgccgca ccgatgcgac gctgctgctt cgcatcaagg acacgccgca
    29581 tcaactgtcg ctgccgcgcg agcatcgcaa gcgcaacgtt cagggcgcct tcgccgtcga
    29641 accgacacgg cgggacgcct tgagaggacg caacgtcacg ctggtcgacg acgtgatgac
    29701 gacgcaggcc accgcggccg agttgacgaa ggtcgtgcgc gccgcgggtg cagcggccgt
    29761 gcatgtctgg gtggtggcgc gcacccccgc gccggggcac tgaccatgtt caacatcgtt
    29821 ctggtgcatc cggagatccc ccccaacacc ggcaacgtga tacggctggc ggccaacacg
    29881 gggtgcgagc tgcatctgat cgagcctctg ggcttctcga tggacgacaa gctgctgcaa
    29941 cgcgccggcc tcgattacca cgagcatgca cgcgtacagc ggcatgcctc ctgggatgac
    30001 ttcatcgaca acaggcaacc cgacctgacg cgctgcttcg cgttcacgac aaggggcagc
    30061 cagacccccg gcgccgtgcg ctggcaagcc ggagactggc tggtattcgg atcggaaacc
    30121 tccggcctgc cggagtcact gcgagcctcg ttcgcccagg accggctggt gaggctgcct
    30181 ttgcttccgg ggcaacgcag cctgaacctg agcaatgcgg tcgcggtcgc ggtattcgaa
    30241 gcgtggaggc agcacggcta tgccggcggc gtgtgaacgc tgatcagagc tcggcgcgcg
    30301 ggtcgcgccc catcagcaac tcgacgccct gactcggggt cacacgaccc tccagcaccg
    30361 ccacgaccgc cgatgtgatg ggcatctcga cgcccagcgc ctgagcgcgg cgcaacacgg
    30421 tggccgcact gaacacgcct tctgcgacgt gccccagatc tgccagtatc tgcggcaagg
    30481 gcttgccctg cgccagttgc aagccgacag tgcgattgcg cgacagatcg ccggtggccg
    30541 tcagcaccag atcgcccagt ccgctcaagc ccatgaacgt ttccgcccgt gcgccgagtg
    30601 ccgtgcccaa tctcaccatc tcggccagac cccgcgtgat caacgcagcg cgggcgttgt
    30661 agccgagctg caggccatcg gccagtccgg tggcaatcgc catcacgttc ttgacggcgc
    30721 caccgacctc gacgcctatc gggtcgtcgg atgtgtagat ccgcaggttg tcacagtgaa
    30781 gcagctcaac cgcctgactg caaagggacg gatcgacgct cgctgcgacc agcgccgtgg
    30841 gctgaccgcg agcgacttcc tcggcaaagc tcggccccga caggacgccg acccgagcgc
    30901 cgggccgcac ctggccagcg acctcatgtc ccagccggcc ggtatccttc tgcagacctt
    30961 tgcacagcca gaacaggccc ggaccctgcg gcccggcggg cagtgcgctt aacaccgcag
    31021 cgagcgcgga catcggcgtc gccacgatca gcaggccgcc acgtgcatgc tcgacggcag
    31081 ccttgaaatc gctcgtgatg tggatcgacg gcggcagttc gaccgcgtcg agatagcgca
    31141 tgttgcgcct gagcgcctgc atgccctgcg cctgcgcggg atcgcgcgcc cacagcatcg
    31201 tggggtgccg ggcactggcg ctgaccgcca gtgcggttcc ccacgcaccg gccccgagca
    31261 cgctgaggtt cacgggtgag cgctcaggtg cccggtgcgc ggatcagacg ctcagttggg
    31321 aagcgcgccg ccgttcgctg cagccttctt ggccgcttct tcagcggctg ccgccttctg
    31381 accttcatac atggcctgga agttgacttc ggtcagcagc accggcggga agccggcacg
    31441 tgtgcagagg tcggagacga tcgcgcgcag gtacgggtag acgatgccag ggcaggcgat
    31501 gctgacgatc ggctgcatct gttccttggg aatgttgcgg atctcgaaga tgccggcctg
    31561 cttggcttcg agcaggaaca gcgtcttctc gcccacggtg gcggtcaccg tgacgcgtag
    31621 cgtcacttcg tagttgtccg ggccaaccgt ctcggcaccg acaccgatgt tgatgtcgac
    31681 ctttggcggg gtctgctcca ggaagatggc aggcgaattg ggctgctcca gcgacatgtc
    31741 cttgaggtag atgcgctgga tgttgaacac gggagcgttg ttgtcttcgt ctgccatgag
    31801 agtctcgatt ggttgctgga tctgcgaccg gagccgcttg cggcccggga ttatggggga
    31861 tggggccagc ctgggctgcc cccgacgcga acgtgcgggg tgcctgcggt cagggctcag
    31921 tgccccgaga gcaagggcaa caagccaccg cgccgatcga ggtcgatcag atcgtcgcaa
    31981 ccgccgatgt gcgcgtcgcc gatgaatatc tgcggcacgg tacgccggcc ggtgcgttcg
    32041 accatcgcgt cccgcgcgga ggggtcaagg tcgatccgga cctcgtcgat cacggtcacg
    32101 ccgcgctctt tgagcaaggc cttggcgcgc aggcagtacg ggcacacctg ggtcgtgtac
    32161 atcgtgacgg cgacgagggg gatgctggaa gtggacatgg cgaactcgcg aagtcagacg
    32221 gaacgttcga tgggcagatt ggcttcgcgc caggccgcca caccgccggc gactgcgtgc
    32281 gtgttctcgt agccccgctt gcggagcagg ccgacgccgc gctgtgcgcg tgctcccgtg
    32341 gggcacaaca gcagcagcgg aacggccttg ttcttgggca ggtcggcgct cgcctcgagg
    32401 ctgccgagcg gtatgttcct ggcgccgccc gcgtgaccgg cggcgtactc ggcggcttcg
    32461 ctgacgtcga tcagcacgcc cttttcccgg ttgatcaggt tgaccgcttc gctcgggctg
    32521 acccgcacgc cacccgttcc gcgagacagc atgggccaaa acagcagcgc accggaaacc
    32581 agggcagccg cgaacagaaa ccagttggcg aggaagaagg tcacgaagcg atccggttgg
    32641 gcagggcgaa ctgcaaaacc ctggattata gaatttgagg gtttccccga catcgggctc
    32701 tcaagcaacc tccgagcacc atccgcctca tgtaccaact cgtgttgatc cgccacggac
    32761 agtccacctg gaacctcgaa aaccgattca cgggctgggt cgatgtcgag ctgacaccgc
    32821 tcggcgtgga tcaggcgcaa gccgcgggcc ggctgctcaa gaagcacggg ttcgatttcg
    32881 acctcgccta cacctccgtg ctgaagcgcg ccgtgtggac gctgtggcac acgctggatc
    32941 aactcgaccg cacctggctg ccggtggtgc actcgtggcg gctcaacgag cgccactacg
    33001 gcgcgctgga aggcctgaac aaggcggaca cagcagaaaa gtacggagat gcccaggtgc
    33061 tgcaatggcg ccgcagctac gacacgccac cgcccgcgct cgacccgtct gatccgcgct
    33121 gcgaacgatt cgacaggcgg tatgcctcac tgaaggccgg cgaggtgccg ctgaccgaat
    33181 gcctgaaaga cacggttgag agggtgctgc cgttctggaa cgacgccatc gtgccttcga
    33241 tctccgccgg caagcgggtg gtcgtcgcgg cacacggcaa cagcattcgt gccctgatga
    33301 agcacctgga caacatctcg gacgacgaca tcgtcggcct cgagattccc aacggcgtgc
    33361 cgatcgtcta cgacctcgac gcccagttcc ggccgatcag ccgacgtttc ctcgaaatcg
    33421 actgacgcgc ggagaccgga tcagacggtc ggcgtcatgc ggggcaactg cgccgcgatg
    33481 tccgccgaga gacgcgtggc tagggcgcgg cgctcgagat cagcactggg ctgcgcagcg
    33541 agaaagctca accgggcgac gagcccatcg gccgaagcga ccgcccacag tgactgcaga
    33601 agcgtcgtgt cgccgatgta ggcagcggca gaactgaccg tctcccggtc atcgctaaac
    33661 ctcaaggcaa tcggctgcac gggtgcctgc gccacgatgg ccgcctgcaa caggttggcg
    33721 tggaacggaa gcacgccatg gccgtcactg gtggtgcctt cgggaaacac cgcgacctgc
    33781 tctcccgcct tcagtgcctg agcgaccagg tgcagcaccc gcagcgcatc gcgggaacgt
    33841 tcgcgctcta tgaacagcgt gccgccgcac gccacgagcc agcccagcag cggccacgca
    33901 cgaatgtcgg ccttcgacac gaagcgcacg ggccggaccg cattgatcgc caggatgtcg
    33961 atccacgaga catgattggc gacgaacagg ctggctcctg acaccggcgt tccggaaaca
    34021 cgcagcccga tccccagcac cgtcagcgct tgtgccgacc aacggcccac atgggccatg
    34081 cgtcgtggtg cgtcgatgaa cgggaagacc acggcgcatg tcagcatccc acgcagaacg
    34141 tgcagcccgc agcgaagcag ccgaagaaca gcccgcaggc ggacccggat caaggcgatt
    34201 cgaatgccac gtgcccggag accagggtgt acctgacgcg gcccggcaac tcgtgcccgg
    34261 tgaagggcgt gtgctttccc tggctcctga gcgcgtccgg gcggacctgc cagtattcgg
    34321 ccggatcgaa caggcagaca tcggccacac caccctcgac cagctggccg gcgctcgatg
    34381 ccaaggatcc aagcgcgtca gcgatcaccc gaaccggcga gatggtgatc gtcgccaacg
    34441 tgcgagccag ttcctgtccg gattgctctc cccacttcaa agccagtccg agcaacagtt
    34501 cgagaccggt ggctccgggc tcggcctcgg cgaagggaag cgtcttggcg tcctcgtcga
    34561 ccggcgtgtg gtcgctgacc agcgcatcga tggttccgtc ggccaacccg gcgcgcagcg
    34621 cgtcccggtc gcggacttga cgcagtggcg gcgtcaggcg catcgcggcg tcgaaatacc
    34681 cgatgtcgat gtcggtcagg tgcaggtggt tgacgctgac atcgcaggtg atcggcaacc
    34741 cctcggcctt ggcgcgtcgc accagatcga cccctgcggc gctgctcagg cggcagatgt
    34801 gcacgcgcgc acgggtgtca cgcaccagtt cgaagagcgt gttcagggcg atcgtctcgg
    34861 cgatcaccgg caccccggtc agccccatgc gcgtggccag cgcacccttg gcggcgaccc
    34921 cgttgccgag gtagccatcc tgggcccgca gccaggtcgt gtagccgaag gtcgatgcgt
    34981 actgcaaggc gcgatgcagc accagcgtgt ccttgagcgc gacttcggca tgcgagaaac
    35041 cgacgcagcc tgcctcggtc agctcggcca tctcggtcag cacctcgcct gccagaccac
    35101 gcgtcaacgc gcccagcggg tacagatgcg actggttcag gttgcgtgcg cggaacttga
    35161 gcatctcgac cagaccgggc tcgtcgagcg gcgggtcggt gtcgggcagg cagacgaggc
    35221 tggtcacacc gccggcgacg gcggcggcca tctcggactc gagcatgcct tcgtgctcgt
    35281 gaccgggctc gcgcagccgc accgccagat cgacgaatcc aggcgcgacc accagaccgc
    35341 gggcgtcaat ggttcgattg gggtggaaat cggcactgac ctggccgatg cgcacgacgc
    35401 ggccggccgc tatcgcgatg tcggtttgtt cgtcacgacc ggaggccggg tcgatcaggc
    35461 gaccgttctg gatcaggatc ttcattctct tcagtcccgc gccatcaggc ggaattgccc
    35521 gcaatcgtcg acatcaccgc catgcgcacg gcgatgccga aggtcacctg cggcaggatc
    35581 acgctctgcg cgccatcggc cacgcgggag tcgatctcga caccgcgatt gatcggcccg
    35641 gggtgcatga cgatcgcatc gggcttggcc agcgccagct tttcctgggt cagtccgtag
    35701 ttcttgaaga actcgcccgc gctgggcagc atggcgccgc tcatgcgctc gttctgcagg
    35761 cgaagcatga tgatcacatc ggcgccgcgg atgccttcgg ccatgtcgtg acagacccgc
    35821 acgcccatct cgcgcaggtc gccgggcacc agcgtcttcg gtccgaccgc acgcacctcg
    35881 ggcacaccca gtgtggtcag cgcatggatg tcggagcgcg ccacgcgcga gtgcacgatg
    35941 tcgccaacga tcgccaccgt caggttggtg aagtccttct tgtagtgacg gatcgtgtac
    36001 atgtcgagca gaccctgcgt cggatgtgca tggcgtccgt cgcccgcgtt gaccacatgg
    36061 acgtgcggcg cacagtgctg cgcgatcagg tagggcgcgc cactctggct gtgccgcacc
    36121 acgaacatgt cggcgtgcat cgcactgagg ttggcgatgg tgtcgagcag gctctcgccc
    36181 ttggcagcgc tggagcgcgc gatgtcgagg ttgatcacgt cggcactcag ccgcttggcc
    36241 gcgatctcga acgtggttct cgtgcgggtg ctgttctcga aaaacaggtt gaaaacgctc
    36301 ttgccgcgca gcagcggcac cttcttgacc tcgcggtcgt tgacgctcag gaaggtgccc
    36361 gcggtgtcga ggatctgatg aatcagcgcg cgcggcaggc cttcgatgga aagcaggtgg
    36421 atcagctcgc cgtgcttgtt gagttgcggg ttgtttctga aattcaagcc gtgcgctccc
    36481 ggatgtcgaa gctgaaacga cccgcgtcat cgcgcacgag cgacaaccgc tgggccgccg
    36541 gcagcgtgat gcgggcagcc gcataggcag ggtggatagg gagttcgcga ccgccgcggt
    36601 cgaccagcac cgccaggatg acgctcgccg gccggccgaa gtcgaagagc tcgttgacca
    36661 cggcgcgcgt ggtgcgcccc gtgaacagga catcgtcgat cagcacgatg tgccggccct
    36721 cgatctcgaa cggcaaacgc gtgtggtcgg cgcccgcggt catgccgcgc gatccgaaat
    36781 cgtcgcggtg cagcgcgctg gagatcacgc cgaactcgcc ctcgagttgc aggtcgcgct
    36841 gaagtcgctc ggccagccag gctccgccag accagatgcc gaccagcacg gtttgcggcc
    36901 gcagcaggcc acgcacaccg gccagcagat cgccgtacag cgcttcggca tcgagttgca
    36961 gagaactcat tcggattcac tccgcaggta ctgttccagg atcaacgctg ccgaagcagc
    37021 atcaaggtct cgcgcaccgc ccgcatgggc ctcggttgtc gtgtagcgct cgtcgacctc
    37081 gtgaacctgc atccgaagcc gcccatgcaa ctggcgcgca aagcggcggg ctcgcagggt
    37141 gttctcgtgc tcggctccat ccgggtggaa aggcacgccg acgaccagcg catccggctg
    37201 ccattcaccc accagacgcg cgatggccgc gaaacggtcc tcgccggtca cagcaatggt
    37261 gcaaagcggc tgagcgctgc gtgtgagcgt gttgccgctg gcgacgccga cacgcttctg
    37321 accgaaatcg aatgcaaggt aggtgcgcag gcgttgcgca gcgccaccga gtgacgcgcc
    37381 tggagcaatg gttccgaggt caggcatgcc cggcatcggc gctgagcatg gcggggtcga
    37441 tccccagcag ggaaagcgcc ttgtcgtagc gctgctcgac cggcgtgttg aagatgatct
    37501 ccggctcggc ctcgacattg atccaggtgt tgcggcccat ctcgtcttcg agctgaccag
    37561 ctccccagcc gctgtatccc agcgtcacca ggatcttcgt cgggccgttg ccactggcca
    37621 gcgcttcgag cacgtccttg ctggtcgtca tttccagccc gccgggaatc cgcagcgacg
    37681 agctgaagcg cccttcctgg tcgtcgagct gctcatgcag gacgaagccg cgttcggtct
    37741 gcaccggccc gccgtagtag acaggcgact ccgcgagatc gacgcgatcg agctccagct
    37801 cgaccttctc gaaaagactc ttcagcttga tgtcgatcgg cttgttgatt accagcccca
    37861 gcgcaccctt gtcggtgtgc tcgcaaaggt agatcacggt gcccgcgaac gtatcgccgg
    37921 cgatgcccgg catcgcaatc aggaactggt tggtcaagtt gatgctgccg aagctcatcg
    37981 ccgcatttta ttcctgcggc acactcttcg gatgacccct tcgctcgatg ccgcactcgt
    38041 ctggtttcgc cgcgaccttc gcgccgagga tcaggccgcc ctgcaccatg cgctgagagc
    38101 ggctcgccat gtctggtgcg tgttcgtgct cgaccgcgac atcctcgacc cgctaccgct
    38161 cgccgatcgc agggtcgagt tcatcgttga cagcctgacc gatctgcatg cgaggctggc
    38221 ggaaatcggt gcggtacacg gcgtgacagg cgccggcctg atcgtgcggc acgcatgcgc
    38281 acgcgacgag atcccggcgc tggcccagcg gctgcgggtg caggcggtct acgccagcca
    38341 tgacgacgag ccccgtgcgc tggaacgcga tgcgcaggta cgcggacggc ttgcccatct
    38401 gggcatcgcg ctgcacacca gcaaggacca tctgatcttc gagcgcagcg aggtgctgac
    38461 cggctcgggc acgccctatg gcgttttcac gccctacaag aacgcgtggc tgaagcggtt
    38521 gacgccggag cacctggcgc cctggtccat cgagccgcat gcggcgcatc tggccgcgtt
    38581 gccaaaagac ctgcccggtg gcccaccgtc gctcaccgac atcggcttcg cgcccaccaa
    38641 tctggcctcg ctgcggatgg ggcagggagc caccggcgcg caggttgcat tcaacgagtt
    38701 cctcacgcgc atcgacggct acgaggacgc gcgcaatttc ccggccgtga agggtccgag
    38761 ctacctgtcg gtgcatctgc gcttcggcac gatctcgatc cggcaactgg cgcgcgaggc
    38821 tcaccggcgc atgcaggacg gttcgcgcgg tgcagaggtc tggctgtccg agctgatctg
    38881 gcgcgacttc tatcaccagg tgctgcacca ccacccgcat gtggtggcgc gcagcttcaa
    38941 gcccgaatac gaccgcatcg tctgggagca tggcaagcac gcggatgcgc tgttcaccgc
    39001 ctggtgcgaa ggacgcaccg gatatcccct gatcgacgcg gcgatggcgc agatcaatca
    39061 gaccggttac atgcacaacc gcctgcgcat ggtggtggcg agcttcctgg tgaaggacct
    39121 gggcatcgat tggcgctggg gcgaacgcta tttcgccgag catctcaacg atttcgatct
    39181 ggccgcgaac aacggcggct ggcaatgggc gagttcgacc gggtgcgacg ctcagccgta
    39241 tttccggata ttcaatccgg tcagccagag cgagaaattc gatccggcag gcaagttcat
    39301 ccggcgctat ctgccgcagc tcgcggccct tcccgatgcg gcgttgcacg caccctggac
    39361 agcgcggccg gtggatctgg ccgcagccgg catagagatc ggtcgaaact ggcccgctcc
    39421 ggtcgtgcag cacgatgctg cgcgcgccag gacgcttgaa cgctacgcgg tggtcaaggc
    39481 aaaccgacct tcggatcgaa cttagaagaa ttcgaagtcg ccaaccttgg tggtatccgc
    39541 cagcagtccc tgagccacca gatcggcgtg actgcacacc cggttgcgac cattctgctt
    39601 cgcgtgatag acggcctgat ctgcccgttc gaacgcgccg ctgggcgtgt ccccgggtcg
    39661 aacggcggtg aagccgatgc tcaccgtgac ccgaccgacc tgcggaaact cgaactgctc
    39721 ggtcagtacg cggaagcgtt ccatcgcctg cgcggcatca tcctctgcgg ggcaacgcat
    39781 cagaaccagg aactcctcgc cgccgaatcg gtaaagccgg tcgtggagcc ggaagctgcg
    39841 agccatcagg cgcgacaaca gcagaagcac ctcgtcgccg atcagatgac cgaaccggtc
    39901 attgacgctc ttgaagtggt cgatgtcgat gacgcctatc cagtagccgc aggggtcccc
    39961 cccgcaacag cgccgtcccg ctttctcggc atcggtatca ggcatcagat cggaggtcgc
    40021 cttcaggaag ctctcgtcga aggtcttgcg gttgagcaac ccggtcagcg tgtcgcgctc
    40081 gctgtaatcc agcagcctgt cgaaattgtg aaagatgcgc agcacgcctt cgatggtccg
    40141 ctgcgcggtc tcgtcgagcg cggcatcggt ctcgatctcg atcactccga cagcatcact
    40201 gccgccgacc aacgggaagt aggcccggcc aggcgcgccg cccacaatga ccaggcgctg
    40261 ttcggtcaac gcctcgaagc gcggctcacc gggctcgagc cggggcaaat tctcgaggtc
    40321 actccacgga aggtcggact tgggcgccgc atcgccacgg cgcaaatgcg cgcggttcag
    40381 ccagcgacga tcttcgacct cgccgacgca gcgatgaatt gtcaccatgc gcggccggac
    40441 catgtccatc agcgaactcg ccagggccag atccagcaac tcgcggtcgc gatggctggt
    40501 cagctcggcg atgtggtcgg cgaacgggat catgtcgaat atcgaagcgg agctgtcctc
    40561 aagccaccac cggaacggcc gtgcatcgcg caatcaacgc cagcaggtcc tcctcggaat
    40621 agggcttgcc caggtagtgc tcgacgccaa gctgcgcggc atagtcgcgg tgcttctggg
    40681 cgatgcgcga ggtgatcatg atcaccggca gatcgctcag gcgcgcatcg gcgcgcaggt
    40741 tgcgcaccag atcgaagcca tccatgcgcg gcatctcgat gtcgctgagc acgatggcgg
    40801 gtcgctcctt tgcgagctgc tcgagcgcgt cgagtccgtc cttggccagc atcactcgat
    40861 agccctcacg cagcagcatg cgttcggtga cgcgtctcac ggtcagtgag tcatcgacca
    40921 cgagcacgag cggcgccagg gttcgggccg ggctctcatc agccgcaggc tgcgcgtggc
    40981 cagccaaccg tgactgcaac gccgactggg tcgcgacacg ggccgagctg ccgtacaggg
    41041 tcgccaacgc caccgggttg tagatcagga cgacatcacc ggaagccatc agggtcatgc
    41101 cggccagtcc aggcatccgg gacaactgcg ggcccaggtt cttgacgacg acttcctcgt
    41161 tcccgacgac gtcatcgacg tggatcacga tgcgctgcga cgcgctgcgt atgaccagca
    41221 cctgagcact gcgcccggcc ggttcggcgc tgcacgaact ggtctgcaac agcgcaccca
    41281 gccagaagaa tggcagcgcg ctgtcatcga cggtgaaact gccgctcagg tgggcctgct
    41341 ccatctcggc ggcaggcacc cgacgcacgc tgacgatcag cggcgaaggt attccgacgg
    41401 tgagatctcc gcaacgaacc atcacgacct gcgtcactgc cgtcgtcagc ggaaggacca
    41461 gcttgaaact ggagcccttg ccggaagtgg tggacgtctc gatgcgaccg ccaatcgcta
    41521 cgacctcgga gcgcaccacg tccatgccga ccccgcggcc ggacagctcg gtcaccttgt
    41581 ccgcggtcga gaagccggcc gtgaagatga ggttggccag atcgccgtcg gacagcgcgg
    41641 cgccttcagt gatcaggccg gtggccacgc ccttggcacg gatacgttcg aggtccaggc
    41701 cccggccgtc gtcgcgcact tcgacactga cctcgttacc gtcctggctc actgccacga
    41761 cgactccgcc ggtggcgtcc ttgccagccg ccacgcgtgc ctccggagac tcgatgccgt
    41821 gcgtcacgct gttgcgcagc aggtgctcga atgccccggt catgcggtcg agcacgcccc
    41881 ggtcgatctc gatcgaaccg ccgacgatgt cgagccggac ctgcttgccg gtttccttcg
    41941 ccgcctgtcg gacgacgcgg tagagccgat cggacagccc ctcgaactcg accatgcggg
    42001 tgcgcagcag gtcgtcctgc aactcgcgcg tcaggcgcgc ctgtgcggcc agttcgtcct
    42061 cggtgctctg cagcgagcgc tgcagcgtgc gctgaaccgt ggcgacgtcg ttgaccgact
    42121 cggccatcat gcgggtcagc tcctggaacc gcgtgaagcg atcgaactcg agcgggtcga
    42181 atgcctgggc cgacaccttg gccgcctcca tgcgggcact gatctgcgtc tcggcctgca
    42241 attcgatgtc gcgcaattgc ccgcgcaggc ggtcgaggtt ttcggtcaga tcggacagcg
    42301 atcccttgat ctcctggacg tccgaagcga tgcgcgaacg ggtgatgctg acctcgccgg
    42361 cctggttgac caggcggtcc agcaagggag cgcgcacgcg caccgctgcc gtggagatcg
    42421 aagccaacgg cgcacgcggc gcttcgacga cgccctcgct ggcaccgaaa cgtgaccagt
    42481 cgacctcggc attcgcagcc gaatcaacgg gccggtgatc gctgtcgaag ccaggggcgg
    42541 aaacaggagc cggtgcagcc tgcgctgcgg aatagtccgc aacggcttcg gtctcctcga
    42601 ccgcagcctc ttcatcgatc gacaccgggg gtgccatctc aacgggtcgg gtgaccgcaa
    42661 ccgcctcggg cgctgcagcc tcttcggtcg aggccgcggc ccgctgaaca gcctcgaacg
    42721 cagctcgcag cacatcgcag cgcccgagca agggttcgac gtcatcggcg gtcagcgcag
    42781 cctctgccga ctgcgacatc aggttctcga tgcggctttc aagccgatgc acgagttcgc
    42841 cgaggcgcat cgcccctgcc agacgcgccc cgcctttcag ggtgtgcagg gtgcgcatcg
    42901 cgccagcggg ctggctggca tcgtccggct gcctgaccca gtcctgcagg cgaatcgcga
    42961 gttcgcccag cagttcttca ccttcttcct cgaagatcgg gaacagctcg gcatcgatcg
    43021 cgtcgatggc gtcgatgtcc tcgtcatcat cgaatggctg cggcgcgcct tcttgagccg
    43081 agcgaatcga ttcggaatcc gggacgggcg attcgtccag cgatttcagt tcagcaacgc
    43141 cgagcgggtc gaggcgcggc acatcggtac tgacgctgaa atcgaggtga atcggcgggt
    43201 cgagcggtgc accttcgctg tcgagcagcg attcggcatc cagttcggca acggccgtgt
    43261 ccagacgctg cgcagaactc agttcgtgat cggccagacg ggccagcagg gcttccgaag
    43321 gctgcttcag gaagcctgcc gcgaactgat gcagcaggtg ccggatttca tcggccgcgt
    43381 cgacgaacag agcgccctct tccgaggtgc ccgagcctat cgcgcaacag cgcgacagcg
    43441 cgtgctcgag cgtgcgggaa agattcgaca gatcggcgaa gcccacggtg gccgaactgc
    43501 cagccagcga atgggccagc gccaccgcgg tatcaccgac cggccgatcg agttccatcg
    43561 cccattcggc gagttcggtg cacagtcggc gcgacagctc atcggcttcg ttcaggtaga
    43621 tattgaacag cgggatgccg atacgcagcg agccgatcac cttgaccgcc tcgtccgcat
    43681 ctggcgtttc atccaccgtc tcgatcagct cggacgcctg cccgatgccg ggctcggctt
    43741 ccagcaaggc cacaggctcg ggttgaaact ccggcgcggc ctcgccctgc tcttcatcaa
    43801 atacgctgaa gggctcggac ccgaggtcca ggttcagcaa ttcgaacgaa ggcgagtcga
    43861 cggcctcgga caactcatcg cgagaaagca gcgaatcatg cggcaggggc tgcgtcgcct
    43921 cgggatcgaa cggctcgaac atcgactgcg cttcgctggc atccagatcc agcgcaagca
    43981 gcctggaatc tgcgggaccg gctttcgccg gatcgcgcag cagcatggtc gaatccgaat
    44041 cgaatgcgac ttcgggtgaa gactcgacac ctgtggcggg caggttgagg tccaggtcca
    44101 cgagcgcatc gaaatccagc tcgtccaggg ccagcacttc tggcatggac tcggcagcag
    44161 gaacctcacc cggcatgtcg agctcgaaat ccgccggcat cgtgaaatca ggcggcaccg
    44221 caccgctgtc agcgtcatcg gctgcggcga atgcgagttc gtgcacgacg ggaggcagcg
    44281 ccggcaccgc ctcgatgacc ggcgcttcag cgccagcctg caactgagca gcggcttccc
    44341 ggatgcggtt cgccgtgtgc gaggtcgcgc gcgaggcggc gatgtcaccg acccagtctg
    44401 cgaactgatc gagcgaccag gcggtgaagt cgagcagcga cgaatcggct gcctgctgct
    44461 ccgaaagccg tgtgttgtag agctgttcgc agacccaggc ggcatcaccg aattcggtca
    44521 ggcccaccat gcgggcgctg cccttgagcg tgtggaacgc gcggcgcacg atcgtgagct
    44581 gctgcagatc ggcaggctgg gccgcgagtt cggcgacggc gccgcgcgct gtctcgaaga
    44641 cctccagagc ttcttcgagg aacacctcgc gcatttcttc gtcttcgacg gattcgtcgg
    44701 caccgacgtc ccgcccggcc gtggttgccg gttcgaggcc gaccgcttca gacgaggtgt
    44761 gcacgaagtc cgccagcgct tcggacagct gatcgcgcgc agcgctcaca gcatcggaat
    44821 cctgtgcctt ctcgagcgcc atgcgcgcct ggttgaccgt ggcggcgagc gccggctggt
    44881 cggccgccgt ggcttcgtgc gacaggcgct cgaggtcgcg cgtgaccgcc tcgatcgaga
    44941 catcctcgcg cgccgcgttg aaggccagca tctgcgcctg ctcgatcagg cgcggctccg
    45001 cgaccgctgg agcgggctcg cccggaatca cgggcttgcg ttcgccacgg cccatcaccg
    45061 gggacaacgt tccggaatcg gcgtcgtagg tgaacagcgt cttggccaga gtgggctgca
    45121 cgctgagcat gtcgatcagg aatccgagcg ctcccaggtt gtcggcgagg cgatcgaaca
    45181 cgccggcctg cggcacacgc gccacatcga cctcggtcga actgagacca tcgacttcat
    45241 cgcgcattcg cagcaacgcg ctggctgcgt ggtccatgcc cagcaccgac agcacacctc
    45301 gcatggccga caggtgattc ggcaccggga tcagcacctc cgggtcggcc gggttgcgga
    45361 agaactggtc gatcagcttc tcggcttcgg acagggtcgc gcgcaactcc tgcaccacgc
    45421 tgcccatggt ctggcggtcg gacacccgtc gatacaggtc ttccatccag ccttcgagcg
    45481 gctgcggcgc ctcgccctgc cgaacactgg ccatgcgctg cgacatgcgc tgcacgcgtg
    45541 cgcccagttc agggtgatcg aattcggcgt cctcgaggca cgcctcgacg taaagcagac
    45601 tggtggccgc ctccatggcc aacggcgcag acggcggagc agcggattgc tgagcctggg
    45661 ccgcggcagc gcgcaactct tcggccagca attcgcctga ggggaacagc cgcttcaatg
    45721 actcgccagc gagcgcgaac tgctcggcca gtccgttcaa tcggtgcatt tccccgccgg
    45781 ccacaccaga ccaggattcc ttggcggcag caacgcgttt gcgcgcttgc gcaatcacgg
    45841 ctggatcgaa tcgaccgagg gcgctgacgt ggtagtcggt tgtgaggtga tggaccaggc
    45901 cgtaggcctg gcggaccgca gccagccgag gtgcccggcg accgtcacca ggcgaatcgg
    45961 cctgtgcaca gaagaacagg agatctcggg ccagccgttc ggacacctcg cgcccgccgc
    46021 gctcgaagat gcgcaactgc gccagcagcc gcgaggccac gcgcttgctg aaaacatcgg
    46081 gagagagcag tccttgcgac tgcgcctcga acatggccgc agccagcttc cacagcgtgc
    46141 ttgtctgcgg gtgatccgtg ccggcaccga gtccggcgca gatgtcactc atgcggctgg
    46201 cagtcgccgg cgttggattg cgcatgatcg acagcatctt gccttcgatg gccgagcggg
    46261 tggctgcatc tggagcgcgc ggcaccgcca actcatcgtc aggcagcggt tgccattgcc
    46321 agtcgcgcgt ccacagatcg gcgggatgca cacggtcggc gccagccagt tcctggacgg
    46381 cgcggtactg cgggaacatc gccagcggcg aaaccgtctt gccggacagc aggcgtgcga
    46441 catagtcgag cacggcaaac gacgcgcttt cgatcgtggc cacggcatgg atgtcgagca
    46501 tgcgcggctt cgctatgcaa cgctgcacag ccgcttcgca cgcgcgcagc agattggctc
    46561 cagcgggcaa accgaccagt tcgagcgcac cgacgccttg atggatgtgc tggcgggcgc
    46621 cgtggatgac cgcgggatcg gcggcatcga catccgagtc gaaggtggtc tcggattcct
    46681 tcaggtggcg ccgcaaggcc ttgtgcgcca gttccagcga gcgtcgcaac tcgtcctgaa
    46741 cccacgacag ggcactcagg tcgctctggc tttccgggtc atcgccccgg ctgtcggtcg
    46801 actgcggatt gcgcaagctg tccatgcgat gccttgtgtc tggtgattct gcgaggacga
    46861 aggagtaggg agcgaaatgc caggcgtggt gctcagacga tcttgaaacg agccaccgat
    46921 tgcttgagtt cctcggccgt atgcgaaagc tcgcgaacca tctgtgcggt cgatcgagtg
    46981 ccttcgccgg tctgctccgt cacagcgaag atgtgctgga tattggccgc caccacgttg
    47041 gccgattgcg cttcgccaag tgcctggctg gaaatatggg tgatcaggtc cgacaactcg
    47101 cgtgacactc ggtcgatgtc gttgagcgcc gtgccggccg cgtctgtcag ccgggtacct
    47161 tcgaccacgc cctgggtcga gcgctccatg gcggccaccg cgtcctgcgt gtcggtttga
    47221 atcgtcttga ccagcgcggc aatctggcgc gtcgcgtcgg ccgagcgttc agcgagccgc
    47281 tgcacttctt ccgccacgac cgagaagccg cgcccggcct cacccgccga tgcggcttgg
    47341 atggccgcgt tcagcgccag gacgttggtc tgctcggtga tgtcggagat cagctcggtg
    47401 atctcgccga tctcctgcga cgattcgccc aggcgcttga tgcgcttgga ggtttcctgg
    47461 atctggtcgc ggatggagtt catgccgccg atggtgtcct gcacggcctt caggccggac
    47521 tcggtcgcgt tcagcgactg gcgcgcgacc tgcgcggtct gctgcgcctg ggccgaaacc
    47581 tcgttgatgc ggccggccat ctgcagcacc gattcgccgg tgtcgcggat ctcgcgcaac
    47641 tgctcgctgg atgcagccat cagttcggtc gaggtggcat cgacgagttg cgtggtttcc
    47701 gtcacacggc tcaccgtgcc ctgcacctgg gcaaccagcg tacgcagctc ttcgacggtg
    47761 tagttgaccg agtcggcgat cgcgccggtg atgtcctcgg tcaccgtggc ctgctgcgtc
    47821 agatcacctt cggcgacgct ctgcagttcg ttcatcagcc gaagaatcgc agcctggttg
    47881 gcgtcgttga tgcgcttgga ttcgtcctgc aggcgctgcg cgtccacgcg ctgctgttcc
    47941 atctgctgcg agcgctgcgc ctgctcggcg ccaaacaccc tgcgttgtcc gaaaactccg
    48001 gcgaacagcg ccaggccgga tacgagcagg agaaccgctg ttccgcgctg ctgcggctcg
    48061 gtgtcggagg ccgcaaacga catcagcacg accgccagga cccccagaag tccgacgagc
    48121 accagcgcac tgagccactg ctgctgctgg gccagtggtc ggttgccgat cagcggcagg
    48181 ccggagcccg cgggcactgc cgcatccggc ggcggctgca gccgcgattc ggcgaaatcg
    48241 gccatccctg agggcgcggc ctcgctgatg atggaagtct gaaagtcgag tgcactcctg
    48301 cccccggcac cttcaagcac gacagtacgt tccatgtcca tgttcatctc cagcggcgac
    48361 cgctcctgca ctgcgtcatg cgcatcggcc gcgtgcgctg actcttcaaa ggaccgtgtg
    48421 tcggacgcat ctcgcccgga cccggtgttt ttccgtttgt ccaaaaaagc catggaggac
    48481 tccccggtcg ccgattgttg gtatgccatg gcggatcaaa ccgccggata tcgccgcgtt
    48541 ctcactgcca gttcgtcgct tcgacgcaga gggcacatgc gtgcgatcag cccacgatct
    48601 tcaggaatgc tgcgtgacgc accaggtcag ccaagctgat ttcctgccag acccggccgt
    48661 cgacgtcacg cagacgctga cccacgaacg acgggcttcc ggcgggcgcc tggctgtcga
    48721 gggtcaactg ctcctcgccg cgcagacccg ccagccgatc gacaagcagc gccgcgttca
    48781 cgtcgagcga tgcattgaat cccaccagca tggcctgatc ccgatggtcg gttccagact
    48841 tgatgccaaa gaaaccagcc agatcgacga cgccatgcag atgtccgcgc agattggcca
    48901 cccccagaaa ccacgggtgc gcgtgggaaa ccggcgccag cgcggtcatg gggaagattt
    48961 caccggcctc cttgagagga aacaggaacc ctcgggtgcc acattcgacg gccagccagg
    49021 atcgccctcg cagcgtcgat cgggcttcct gcagacgttc agccagacga tgctggagct
    49081 cacgcaatgc ttctttattg gccatgagcg agtcgcgttg tagccacgta ggcgtcagat
    49141 cggaaacgac ggagggcgcc gaagcgccag ggtggtgagc gagccgaagc ggtcagccga
    49201 aagcacgaat cttggccgcc agttcgtcgg cattgaccgg cttgaccagg taatcgcgtg
    49261 ccccctgccg gatgccccag accttgtctg tctcttgatt cttgctcgtg cacatgatca
    49321 cgggcacccg cgagaagcga ggatcccggg tgatcgcacg agtcagctgg aaaccgtttt
    49381 gcccaggcat gacgacgtcc atcaggatca ggtcgggcgt ctgctcttcc agacgccgca
    49441 tggcctcttc gccgttctcg gccgttcgca ccgcatagcc ctgcttcgtc agcaagcccg
    49501 agacgtgata aagctccgtc ttcgaatcat cgaccagcag gatgttcttg attgccattt
    49561 cttgtcctca ggagtcgttt tgcggcgcac gggctcggat caagcaatcc gcgcgccgtc
    49621 aaaaccatga caaaacgcgt cagtcaacga ccgcaggggc gtcttcgccc aggtgcaggc
    49681 gcaccgcgcc caacaactgg tccttcgtga atggcttggt aaggtagtcc tgcgaaccga
    49741 ccatgcgccc gcgcgccttg tcgaagagcc cgtccttgga agacagcatg atcaccggca
    49801 catcggcaaa ccgggcattg cgtttgatga tcgcgcaggt ctggtagccg tcgagacgcg
    49861 gcatcaggat gtcgcagaag atcaacgcgg gagcgtggtc gttgaccttg gaaagcgcgt
    49921 cgaagccgtc ctcggccaga tagacgtcgt atccgccctg cttgagaaag atctccgcgc
    49981 tgcgacggat ggtgttgctg tcatcgatga ccagaaccct ggccttgggg gcatctgccc
    50041 cggtgtcctc gttcactgac attctcctca tcaggacgcg cacgtcgcat cccgcctgtc
    50101 gggttatcgg tcagatttgc accatctcaa agtcttcttt tcgtgcgcca cattccggac
    50161 acgtccaatt cattggaact tcggcccagc gagtgcctgg agagatgccg tgttcctcgt
    50221 ctccgacggc ctcgtcgtag atccatccgc agatcaggca catccaggtt cgagtttcag
    50281 tcacagcagt ttcaaaagga aacacggaac gaggctcgcc atcggcggcg ggtgcggtcc
    50341 agtgcggcct catggcgtca ttacaatgaa aacgattcta gcgacgcggt atttgaggcg
    50401 cgcagacttc ggggtgccgt tgttgttcgg caccgtcatt ccgacccgat tcgccggtca
    50461 tcgccctggg gcgcgccgca tcttcctcac ccgagtctcc cgccatgacc gcacaaaccc
    50521 aaccgacagc cgatgccgct gacgagttgc aggaagctcc ggctcctgct tgcgtgatga
    50581 gcttcaacgc ctcggatgcc accggggccg gcggagtgag cggtgacgtc gcgaccatcg
    50641 ccgccatggg cgcccacgga ctgccggtga tcacatcgat agtgatgcgc gacaccgcgg
    50701 aggtgttcga ccaccatgcg ctggagcccg acgtcgtcgc cgaacaggcc cgctgcgtgc
    50761 tggaggacat cacgatcgcg gcctggaagg tgggattcct cggctctgcc gagggcgtcg
    50821 gcgcggtagc ggaaatactg tccgactacc ctgacgtacc cctggtggcc tacatgccca
    50881 acctgtcgtg gctggacgag gatcagcagc aagcctacct ggacgcgttc cgcgaactgg
    50941 tgctgccgca gacagaggtg ctgatcggca gccaccagac cctgaccgac ttcctgctcc
    51001 ccgactggga cggcgagcgc ccgacttcgc cacgcgaact ggcggtggcc gcatccgagc
    51061 acggcgccaa tttcgtgctg gtgaccagcg tgctggtcac caagggcaag agcgccgacc
    51121 agtacctgga caacgtcctc gcgtcgccgc aaggcgcagt gaccggcgag aaattcgagc
    51181 gcttcgatgc cagcttcaac gccgccggcg acacgctctc tgcggcgctg gcctcgctgc
    51241 tggcttccgg cagcgaactg cacaccgcgg tcggcgaggc gctggccttc cttgaccagg
    51301 cgctcgacgc cggtttccgg cccggcatgg gcaacgtcgt ccccgaccgc ttcttctggg
    51361 cgctggcgcc cggcgaggaa gacgagggtg gcgagggaga cccctccgag acagaaacgc
    51421 cgggaacccg tcacattcac tgagtccctg acgccccggc gcccggggcg accgtcaacg
    51481 ctcccgcaga ccgccatgac ttccaccaac gcacaactgt tcgagcgcgc ccagcgcgtc
    51541 atccctggcg gcgtcaattc gccggtgcgc gcattccgcg ccgtcggagg cacgccgaga
    51601 ttcatcgcgc gcgcgcaggg gccgtacatc ttcgacgccg agggcacgag atacatcgac
    51661 tacatcggat cctggggacc gatgatcctt ggccacggac atcctgccgt gctggatgcg
    51721 gtgcagaagg cagccatgga cggcttctcg ttcggggcgc cgaccgagcg cgagatcgaa
    51781 ttggcagaag aaatcctgaa gctggtgccc agcatggatc aggtgcggct ggtcagttcg
    51841 ggcaccgagg ccgcgatgag cgcgctgcgc ctggcacgcg gcgcaaccgg acgcgacaag
    51901 atcgtcaagt tcgagggttg ctatcacgga catgcggacg ccctgctggt caaggccggc
    51961 tcgggcctgg ccaccttcgg caacccgacc agtgccggcg tgccggaaga cgtcgtcaag
    52021 cacaccgtgg ttctcgaata caacaacgtc gcgcaactcg aggaagcctt tgcgctgcac
    52081 ggcgacggcc tggcctgcgt gatgatcgag gcgatctgcg ggaatatgaa cttcgtccgc
    52141 gccagcgtgc cgttcatgac ccgtctgcgc gagctgtgca ctcagtacgg cacgctgctg
    52201 gtgttcgacg aggtgatgac cggctttcgt gtcgccctcg gcggcgccca aagcctgtac
    52261 gccaagctga ttcccggctt cgctccagac atcagcgtct tcggcaaggt gatcggcggc
    52321 ggcatgccgc tggcggcttt cggcggccgg cgcgcggtga tggagcagtt ggcccccctc
    52381 ggcccggtct accaggccgg aacgctgtct ggcaacccgg ttgccacggc ctgcggcctg
    52441 gcgacgctgc gcgagatcca gaagcccggc ttcttcgacg cgctgactgc cagcacccgg
    52501 caactggtcg acggcctgaa cgcggcagcc cacgccagcg gcgtgccgat ggtcaccgac
    52561 agcgaaggcg gcatgttcgg attcttcttc gcctccaagc tgccgcagaa ctacacgcag
    52621 gtgctggcca ccgacaaggc gcgcttcaac cacttcttcc acgcgatgct cgatcgcggc
    52681 gtgtacctgg ccccggcctt gtacgaggca ggatttgtca gttcggcaca ccggcccgac
    52741 gacctgctcg ctacggccac cgcggcgaag caggccctgc agtcctgaaa cccagacccg
    52801 cacgccagcg ctctcgacga catccatgca catccacatc ctcggcattt gcggcacctt
    52861 catgggaggc ctcgccgccc tggcgcggga ggcaggacac caggtcaccg gctgcgacag
    52921 cggcgtctat ccgccgatga gcgaccagct gcgggccctc ggcatcgaac tggtcgaggg
    52981 ctacggggct gatcagctgg gcgtgaatcc cgatctgttc gttgtcggca acgtggtgac
    53041 acggggcaac ccgctgatgg aagcggtgct caacgccgga gcggcctaca tcagcggacc
    53101 ccaatggctg gcagagcatg tgctgcaggg gcgacacgtg ctcgcagtgg cgggcacgca
    53161 cggcaagacc acgaccaccg cgatgctggc ctggatcctc gagcaagcgg gcctgcagcc
    53221 gggcttcctg gtcggaggcg ttcccctcaa tttcggcgtt tcggccaggc tggggtccgg
    53281 cgcaccgttc gtcatcgaag ccgacgagta cgacaccgcc ttcttcgaca agcgcagcaa
    53341 gttcgttcat taccgagccc gcaccgcggt gctgaacaac ctcgagttcg atcacgccga
    53401 catcttcgag aacctggccg cgatcgagag gcagttccac cacctggtgc gcaccgtgcc
    53461 gttacagggc cgcctcgtgg tcaacgcccg cgacgaagcg atccagaggg tgctcggcat
    53521 ggggtgctgg agcgacgtgg tgcgcttcgg cgcgcgcaag gaaacccccg gcgagctgcg
    53581 cgcgcgcggt gaacctcatg cattcgatgt cttgcgcggg agcatgaaga tcgcacgtgt
    53641 cgaatggtcg ctggcaggag agcacaacca gctgaacgca ctggctgcga tcgcggcagc
    53701 cgatcacgtc ggcgtgccgg cggacgtcgc agcgaaggca ctcgccagct tcgagaatgt
    53761 gaggcgccgt ctcgagctgc gtggcagcgc ggccggcatc agcgtctacg acgatttcgc
    53821 ccaccacccg actgcaatgc ggaccacgat caatggcttg cgtcgccagg tcgacgcgcg
    53881 ctggaatccg gcgacccagc cgcggccgcg catcctcgcg gtgttcgagc cgcgctcgaa
    53941 cacgatgaag ctcggcacga tgaaggcgca acttccgtgg tcgctcgaag aggccgacct
    54001 ttccttctgc cacagcggtg gcctcgactg ggacgctgcg caagccctgg caccgctcgg
    54061 caagctggcc gcggtctgcg acagcatcga ccaactcgtc tcgcgcgtag cagccaccgc
    54121 gcagcctggt gaccacgtgc tgtgcatgag caacggcggc ttcggtggcg tgcaccagtt
    54181 gctgctggac gcactggccc gcaaagcctg atgccggcga ccgcctcgcc cgcgatcacg
    54241 cacctgctct acctgcacgg attccgctcg tcgccgcact cgaagaaggc tcggcagttc
    54301 ggcgactggg ttcgtgccca tcggcccgac ctgcactggt ggtgccctca gttgccaccg
    54361 tcgccgagcg acgcgctggc gttgctgatc gaaggcacga aggattggcc gctcgcacac
    54421 gccgcggtgg tcggcagctc gctgggcggc ttctacgcga cggtgctcgc cgagcgatgg
    54481 gcccggtccg atgaacgacc ggacggcgat tcagtacgcg tggcgctgct caatcccgcg
    54541 gttcatcctg cgcgtgacct cgccgcccac atcggcgaga ccacgtcctg gcacgatggc
    54601 gacaggttct tcttccggcc ggaattcatc gacgaattgc agcaactgtc gccggatcag
    54661 atcacgcagc cgcaacgcta ccttgccgtc atcgcaaagg gcgacgaggt gctcgactgg
    54721 cgggaaatga gctcgcggta cgccgactgt tcgatccggc tgctcgaagg cagcgatcac
    54781 gccctcagcg atttcgacga ccacctgaaa gcgacgatcg acttcctggg cctggaagca
    54841 ggcgccgcct gatcaccagt tgatgatggt cttgaccacg ctgccccaga ccacgatgtt
    54901 gaaccagagc agtgcaacag cgaatgccca gcgccggaac atccgggtgc gcttgagcca
    54961 gagatcgatc ccggtggcga tgaatacgcc gaagcacagc aggctgatgc ctcccgtcag
    55021 ccccaggatg ctggccatca cccagtcaaa cggcctcata tcgtagaaat ctctctcagt
    55081 tgggtggcca gacatggccg gttgatgccg cgagatgaca tcacacagcc accacgaatc
    55141 gttcgaggat cagcacggca aagaatccaa gcgctgcgat caccgcccac ttgatgatgg
    55201 tgatgcccag ctgacggtaa cgcatctggc ccgtgccgac gtacatcgcc aggcacacca
    55261 gcccggcgac gagcagcagc aacagcaccc atcggaagat cagcatcacc aggcaggggc
    55321 cagttcggcc aggccggccg gggctttcga tgcatcgccg aaggtcacga tgtcgtagca
    55381 gcccggctcg gcaagcacgg cgcgaaccag cttgttgttg agcgcatgtc cagacttgaa
    55441 ggcactgtaa gcgccgatca acgggtgtcc gatgatgaac agatccccga tcgcatcgag
    55501 gatcttgtgc ttgacgaatt catcgtcgta gcgcaggccg ccggagttga gcaccttgta
    55561 gtcgtcgacc acgaccacgt tgtcgagact gcccccgagg cccagcccac gagagcgcat
    55621 caactcgacg tccttggtga agccaaaggt gcgcgcacgc gcgatctcgc gcttgtactg
    55681 ccccgagccc atgtcgaaca ccacatgctg accggtctgg ttgacggccg gatgatcgaa
    55741 agcgatctcg aaggtcagct tgtagccgtc gaacggctcg agcctcgccc acttcaggct
    55801 cggcccctcg ccctcacgca cctcgaccgg cttcaggatg cgcaggaagc gcttcggcgc
    55861 atcctgcaac acgatgcctg cactctgcag caggaatacg aacgacgccg ccgagccgtc
    55921 gaggatgggc acctcttcgg cggtgatgtc gacgatcagg ttgtcgatgc ccaggcctgc
    55981 acatgccgaa agcagatgct cgacggtgtt gaccttcggc gcccccggat cgccgcatgg
    56041 cgacagcgct gtcgccatgc gcgtgtcgca caccgagtgg gcatgcaccg ggatgtccac
    56101 cggttgcggc agatcgaccc gccgaaagac gacgccgctg tccgccggcg ccggacgcag
    56161 cgtcagttcg acccgctggc cgccgtgcac gcccaccccg accgcgcggg tcagggattt
    56221 cagcgtgcgt tgggcgagca tcatttcatt ctagggacgc aagtgctccg tctccccggt
    56281 ggggattggg agcgaactcc ggcatcggcc tatcgagatc aatcggcctg ctttctgagg
    56341 aaggccggga tctcgatctc gtccatgccg ttgctggcca gcgcatcgac cttggcagct
    56401 gcggtgcggg tgcgccacac gctcggggtg ctcagcgatc cgtagtcagg ggagttgtgc
    56461 ccgccctgca ttccgagccc gggcgcattc agcgggttgt tcaggatcgg caggttatcg
    56521 gtgccggtgc gctgcatcgg cgcgctgtgg acgacgctga tcggcgcctg ctggcggcgt
    56581 gccgggctca ggccggtggc gatcaccgtc acgcgcagct tgtcgcccag accttcgtcg
    56641 taaacggtac cgaagatcac gtgtgcgtct tccgcagcgt agcggcggat ggtgttcatc
    56701 gcgttcttgc tttccgacag cttcagcgtg ctgcggttcg cggcgatcag caccagcacg
    56761 cccttcgcgc ccgacaggtc gattccctcg agcagcgggc acgccacggc agcgtcggct
    56821 gccttcgtgg cgcgatccgg gccgctcgcc tgcgcggtgc ccatcatggc cttgcctggc
    56881 tcgctcatca ccgtcttcac gtcctcgaag tcgacgttga ccatgccgtg catgtgaatg
    56941 atgtcgctga tgccgccgac ggcgttcttc agaacgtcat tggcctcggc gaaggcctga
    57001 tcctgcgtca catcgtcgcc cagcacctcg agcagcttct cgttgagcac cacgatcagc
    57061 gaatcgacat tggcttccag ctctgccaga cctgcgtcgg cggccttcat ccggcggttg
    57121 ccttcgaagt cgaacggctt ggtcaccacg ccgacggtca ggatgcccat ttccttcgcg
    57181 atgcgggcaa tcacaggtgc agcgccggtg ccggtgccgc cgcccatgcc ggcggtgatg
    57241 aacagcatgt gcgcaccact gatggcctgg cggatgcgct cgtcggcttc cagagcggcg
    57301 gccttgccgg cctcgggctt ggcacccgca cccagcccgg tggtgccgag ctggatcagg
    57361 tggtgcgcgg tggaacggtt cagggcctgg gcgtcggtgt tcgcgttgac gaactccacg
    57421 ccctgaacgc cctgcgcaat catgtggtcg accgcgttgc cgccgccgcc gccgacgccg
    57481 atcaccttga tctgggtgcc gaggtcaaat tcttcgatca tttcgatggc catgtgatgc
    57541 tccagtgctt gtctgcagtt gccgtgttga atagagaacc gggatggatc ccgtgtttgc
    57601 cgtgcgtctt gtcttcttga tgtcagggtc gcattcgtgt cgtgccggga ggcggtcgcg
    57661 ccgggggagc acggttgccg cccggatggc aaccgcgttc ggtctggtcg tcgatggtgg
    57721 ggtgattcat cgggggtccg tgtgctgcgt gttcagaagt tgccgaggaa ccagtcgcgg
    57781 gcgcgcccca gcacggtctt caccgagccg gcctgctggg cggcgcgcag gccgcgcgag
    57841 cgagcaatgc ggccctcctc gagcaggccc atcacggtgg ccgaacgggt gttcgcgacc
    57901 atgtcctgca gggcaccgtg gtacagcggg atgcccttgc gcaccggctt gaggaagatg
    57961 tcctcgccga gctcgaccat gccgggcatc accgctgcgc caccgcagat cacgatgcct
    58021 gacgacagca gctcctcgta gccggactcg cggatgacct ggtgcaccag cgagtagatc
    58081 tcctcgatgc ggggctcgat cacgccggcc agcgcctggc ggctcaacat gcgtggcgcg
    58141 cgatcgccga gtccgggaac ctcgagctgg tcgctcggat cggccagcag ttgcttggcc
    58201 accccgaact cgaccttgat ctcctcggcg tccttcgtcg gcgttcgcag cgccatcgcg
    58261 atgtcgctgg tgatcaggtc gccggcgatc gggatcaccg cggtgtggcg gatcgcgccg
    58321 tcggtgaaga tcgccacgtc ggtggtgccc gcgccgatgt ccaccagcgc cacgccgagg
    58381 tccttctcgt cctcggtgag cacggcgtgg ctgctggcgc tggggttgag caccagttgc
    58441 tccacctcga ggccgcagcg gcgcacgcac ttgacgatgt tctcggccgc gctctgcgcg
    58501 ccggtgacga tgtgcacctt gacctcgagg cgcccgccgc tcatgccgat cggctccttg
    58561 acctcgtggc cgtcgatgac gaattcctgc gcctgcacca gcagcagccg ctggtcgttg
    58621 gggatgttga tcgccttggc ggtctcgacg acacgcgcca cgtccaccgg cgtgacctcc
    58681 ttgtcgcgca cgatcaccat gccggtgctg ttctggccgc ggatgtggct gccggtgatg
    58741 ccggtgtaca cgcgggtgat cttgcagtcg gccatcatct cggcctcctt caaggcctgc
    58801 tggatgctct gcacggtggc gtcgatgttc accacgacgc cgcgcttgag cccgtgcgtc
    58861 ggtgcactgc caagaccggc gatgcgcagc tcgccgccgg gcagcagttc ggccacgacc
    58921 accatcacct tcgcagtgcc gatgtcgaga ccaaccacca gatccttcaa ttccttggcc
    58981 atccgatcct ctcgctcaat tccggttgcc cgcgcctggt ccggtgccag ggttcagcgt
    59041 tgtcgtgatt ccccgcaagc gcagggcgta gccgtcgcgg tgccgcagat cggcatacac
    59101 gagcggtgct tgaaactgtt cggtcacctg gctgaccgtg ccgacgaagc gctcggcccg
    59161 cgccaccacc tcgtcctcgc tgccccggcc cagttcgacc ttggcgccac tgtcgagctc
    59221 ggcacgccac gatgcacgac tcgacagtcg cagcgactcg acacggcggt ccagccgccc
    59281 gaaaaccgac tgcaggcggg acagcatcgc gagcatcccg gctgaactgc cttcgggacc
    59341 ctcgagcaca ggcagtcctt cttcctcgac atcgccgacg ctcgcctcga acacctcgcc
    59401 catcgtgttg acgagctggt cggtcgcacc gccgccacgc cacagggcgg ccggccggtg
    59461 ctcctcgagg cgcaccttca atcggttggg ccacacgcgc tgcaccacgg cgcgacgcac
    59521 ccatggcacg gactcgaagg cacgctggtc gacctgcagg tcggtggtga agaagttgcc
    59581 ccgcagcttc ggaaccgcat tgacacggat cgtcgccacg gtattgcgac tcaggtcgcc
    59641 ctcgatgccg atcgaacgca gcgcgaacac gggctgacgg gtcagcaggt gcagcgcggt
    59701 ggcgcccagc agcaaggcgg ccagcacgaa caagccgttc gcggtgtatc gcatccaccg
    59761 aacgtcggcc ggcaacgcag gtaccgacgc gcggtctcgc gcggctgaca tcgatcggac
    59821 gaagggggtg ctggacatgg agcgttcagg ccttcacgtc tgtcgcagcc gacaccggag
    59881 ccgccggccc atcaagcctg gcctggctga gaatcagaag gcagagctgg tcgtatccga
    59941 tgccgacgga ccgcgccgac atgggcacca gcgagtgcga cgtcatgcct ggcgaggtgt
    60001 tcatctcgag caggaacggc tgacggtcag ccgcacggat catcaggtcc gcgcggcccc
    60061 agccgcggca gccgagcgtc cggtacgaag cgagcgccag acgctggatc tcgcgctcct
    60121 cggactcggg aagcccgctc gggcacatgt actggacggc gtcggtgaag tacttgttct
    60181 gatagtcgta ggcgccttcc ggcgcccgga tgcggatcac cggcagcgcg tgcgcatcgg
    60241 cgccttcgcc gatcaccggg caggtgactt cctcgccgtc gatgaactcc tcgcagagca
    60301 catcggcgtc gtatttcgcg gcgagcacca ccgcatccag catctgacga tccacgtctc
    60361 cgccaccggt cactttcgtg acgccgatcg atgaaccctc gcgcggcggc ttgacgatca
    60421 gcggcaggcc cagctggccg ggcacgcggc gcacctgctc gatgtcgtgc tcgcctggcg
    60481 gcagccacac atggcgcggc gtgggcagtc cctcggacag ccagatgcgc ttggtcatga
    60541 ccttgtccat cgccaccgcc gacgccatca cgcccgagcc ggtgtacggg atgcccatca
    60601 gctcgagcgc gccctggacc gtgccgtcct ctccatggcg gccatgcagt gcgatgaagc
    60661 agcgggcgat gccgagcttc ttcagatcgg agaggtcgcg ctcggacggg tcgaaagact
    60721 gcgcgtcgac gcccagcgac tgcagcgcag acagcacacc gtggcccgac atcagcgaga
    60781 tggctcgctc ggccgaggta ccgcccatca gcaccgcgac ctttccgagg gctttcggat
    60841 ccacgttcat cggctgccct ccatcgtgac cgcagcggcc gcatccgtcc ggcgctgcag
    60901 atcgagcacc tgcgcgggca cgccacctat cgatcccgcc cccatcgaga cgacgacatc
    60961 gccagcccgc gcgtgatcga ggatcgcctg cggcatcgcg gcgacgtcgt cgacgaacac
    61021 cggatcaacc ttgccggcca cgcgcagcgc gcgggccagc gcgcggccgt cggcggcgac
    61081 gatcggcggc tcgccggcgg cgtagacctc ggtcagcagc accgcatcgg cactgccgat
    61141 gaccttgacg aagtcctcga agcagtcgcg ggtgcgggtg tagcgatgag gctggaaggc
    61201 cagcaccagg cggcgcccgg ggaatgcgcc ccgtgccgcc gacagggtgg ctgccatctc
    61261 gaccgggtgg tgaccgtagt cgtcgatcag cgtgaagctg ccagcggatt cgtcagccgc
    61321 cgcggtcgcc cgaacctcgc catagcgctg gaagcgccga tcgacaccgc ggaagtcggc
    61381 cagaccctgc accatcgccg catcgggaag ctccagctcg gtggccaccg caatcgcggc
    61441 cagcgcgttc agcacgttgt ggtcgcccgg caggttcagg gtgacatcga ggtcgggcat
    61501 cgcgatgccg ttgcgacgct gtaccgtgaa gcgcatgcga ccggcacgcg cctgcacgtc
    61561 gaccgcgcgc accatcgcat cggcaccgaa gccgtaggtc acgaccgggc gcgacagcat
    61621 cggcatgatc gagcgcacgc ccgggtcatc gctgcacacg atggccgcgc catagaacgg
    61681 catgcggtgg acgaactcga caaaggcctg cttcagccgc gcgaagtcgt ggccgtaggt
    61741 gtccatgtga tcggcgtcga tgttggtcac caccgccatc accggcagca ggttcaggaa
    61801 cgacgcgtcg gattcgtcgg cctcgacgac gatgtggtcg cccgagccca gccgcgagtt
    61861 cgcaccggct gcattgagcc gcccgccaat cacgaaggtc gggtccagcc cggcctcggc
    61921 cagaacgctc gtgacgagac tggtggtggt ggtcttgccg tgcgttccag cgatcgcgat
    61981 gcccttcttc aggcgcatca gttcggccag catcacggcc cgaggcacga ccggcacgcg
    62041 acgagcacgt gccgcagtca cctcggggtt gtcggccttg accgcggtcg aggtgacgac
    62101 cgcctccgcg ccgtcgatgt tcgatgcatc gtgacccacg gccacgcgga tgcccagcga
    62161 tgcgagccgg cgggaggtgg cgctgtcgct ctggtccgag ccggacaccc ggtatcccag
    62221 gttgtggagg atctcggcga tgccgctcat gccggcgccg ccgataccga cgaagtggat
    62281 gtgcttgacg gcgtgtttca tgctgcgatc aaaccttcga gttcgtcagc cacacgcgat
    62341 gcagccctgg gacgggccag cgctcgcgcc ctgctggcca tcgcgagcag cgagtcgcgc
    62401 gtcagcccgg ccagcacatc ggccagatgg cgcggcgtca gttcgttctg cggcaattgc
    62461 aaggccgcac cgtgcgacgc catccactgc gcgttgtcgc gctggtgcga cgtggtgctc
    62521 accaccagcg gtacgagcac gctggctacg ccggcggcgc acagttcgct cacggtgatc
    62581 gcgccggcgc ggcagacgat caggtcgcag tcggcgaggc gcccggccat gtcgtcgatg
    62641 aacggcagca cctcggcgtc caccgtggcg cctgcgtatt cggcctgcac cgactccgca
    62701 tgcgcagcgc ccgtctgatg cgtgacccgg ggccgctgcg ccggaggcat cagcgccagc
    62761 gcctgcggca ggcaccgatt gagcacctgg gcacccaggc tgccaccgac gaccagcaga
    62821 cgaagcgggc cgtcgcggcc tgcgaagcgc gtggtcggcg cgggcaggct ctcgatctcg
    62881 gcgcgcaccg ggttgcccgt gacaacgccg tggcgtgcgc cttgcgctgc cggtccgtcg
    62941 aacccgaagg ccacacggtc ggccaccggc agcagcgcgc ggttgctcat cagcagcgcc
    63001 gcatcggcgt tgaccagcat cagcggcttg ccgagcagcg atgccatcag gccggcggga
    63061 aagcagagat agccgcccat gccgagcacc acctgcgcgc ggcggcggcg gatgatgccg
    63121 aggcaggccc agaaggcgcc gagcatccgg attccgccgg tcagcgtgtg gaacagtcct
    63181 tttccgcgca ccccggtgaa ggcgacggta tcgagcggaa tgcgggtggg cgggaccagc
    63241 ctgttttcca tgccggtggg tgtgcccagc cagctcacgg tccagccgcg ctgctgcatt
    63301 tccttcgcca cggcgaggcc ggggatgacg tgaccgccgg tgccggctgc gaccacgatc
    63361 aaatgtttgc cctgggtcat gcttgacctc tcggcatggt ttgcattgct cggtcagacc
    63421 gcgccgggcc gaaaccccgg cgcggttcat cgcaccggcg cgctgcagcg aaaggcaaac
    63481 gaaccatcat gcccgccctc cccgcatcaa ctgccggttc tcgatatcga cccgcgccac
    63541 gatcgccagc gcgatcatgt tcatcaggat cgccgatccc ccgtagctca tcaacggcaa
    63601 cgtcagtccc ttggtgggca gcacgcccag gttgaccccc atgttgatga aggcctgacc
    63661 gcccatccag atgccgatgc cctgcgcata gagccccgca tagacccggt ccagcgcgat
    63721 cgcctggcgg ccgatgtgga acacgcgccg cgacagccag aagaaggcga cgatcacgca
    63781 ggccacgccg acgaagccca gttcctcgcc gatgactgcg agtaggaagt cggtgtgtgc
    63841 ctcgggcagg tagtgcagct tctcgacgct ggcccccagg ccctgcccga agaactcgcc
    63901 gcgtccgaac gcgatgagcg aatgcgacag ctggtaggcc ttgcccagcg tgtacttctc
    63961 gtcccagggg ttcaggtacg cgaagatccg ctcgcgacgc cagtcactca gcgtgatcat
    64021 cagcacgaag gcaccgatca gtaccgaggt gatcagcagg aacatgcggc cgttgacgcc
    64081 ccccaggaac aggatgccca tcgcgatcat cgcgatgacc aggaacgcac ccatgtccgg
    64141 ttctgccagc agcagcagtc cgaccaccgc cacggcgaat gccatcggca gcaccgcctg
    64201 ccagaagttc tcgcgcgcgt ccatcttgcg caccatgtag ccggcggcgt acatcgccat
    64261 cgccagcttg gcgagttcgg agggctggaa gttcaggaag cccagcggaa tccatcggcg
    64321 tgcgccattg actcccttgc cgatgaacgg gacgagcacg agcagcagca ggatcagtgc
    64381 cccgacgaag atccatggag ccgacttctc ccagaaactc accgggacct gcaccgcgac
    64441 cagcgcagcc agcaccgcga tcagcagcga gaccagatgg cgcaccagga aatgcgtcgg
    64501 cgaatacagc gtgaacttcg ggttgtccgg catcgcgatc gatgcgctgt agaccatgac
    64561 caggccaagt gccagcagcg ccagcacgat gcacaggagc gcctggtcga atgcaagcag
    64621 gcgagcaggc gccccggacg acacattgat ccagtcgcgc accgggatgg ccgtgctctc
    64681 gctggcgtcg gagcgccaca ggccggaaac gcgggccctc agggaaccga accgcggcag
    64741 gcgcaaggtc gtcatgcggc cacccccgca tccgccgcga tcgcgtggac ctcgtcgacg
    64801 aacacctcgg cgcggtgcgc gtagttgcgg aacatgtcca ggctcgcgca ggccgggctg
    64861 agcagaaccg catctccgga cttcgcctgc tcatgggccc agcgcgtggc agcctcgagc
    64921 gtggcgtggc gcttcagcac gaggccgtcg attccggaca gcgccgcctc gatcagcgcg
    64981 gcgtcgcgcc cgagtgtcgc gacggcgcgc gcatgacggc gcaccggctc tgccagcggc
    65041 gtgaaatcct ggcccttgcc gtcgccgccg aggatcacga cgatccggga cggcgcgcgg
    65101 tcggcgccca gcccttcgag cgccgcgacg gtcgcgccga cgttggtgcc cttgctgtcg
    65161 tcgtaggcgt cgacaccctc gatggtggca acgtgcgcga cgcggtgcgg ctcaccggcg
    65221 tactcgcgca atccgtgcag catcggcgcc agcggacagt cgatcgcggt ggcgagtgcg
    65281 agtgcagcca tcgcgttggc ggcgttgtga cgcccccgca cccgcagcgc atcggccggc
    65341 atcaggcgct ggatgtgcag ttcctctgct tcgacttcgg tgccggcacg cacccgcttg
    65401 cgcggaaagc tgtcgtcgga ttcaagggca cgcaccagcc aagccatgcc gttctcgctg
    65461 accagaccga agtcgcccgg gcactgcggt gcgtcgaggc cgaagcggac gatctggcgc
    65521 tcggcgggct tgggcgcgcg ggacctgacg gtgcccttgg ccgggatgtc cgccggctga
    65581 accgatcgcg ccagcgcttc gacccgcgga tcatcgcgat tgaccaccat caccgcctcg
    65641 cggccccaga cacgcgcctt ggcggcggcg tagttctcca tcgatccgtg ccagtcgagg
    65701 tggtcctcgg tgaggttgag cacagccgcg gcgtcgggtt cgaagccctt cgcttcatcg
    65761 agctggaaac tgctgagctc cagcacccag acctgaggca ggctgtcgcc gtcgatcgca
    65821 tcggcgagcg tctgcagcat cgtcggcccg atgttgccgg ccacggccac ctgcctgccg
    65881 gcgcgctcga ccagcagcgc ggtcatcgcg gtggtcgtgg tcttgccgtt ggtgccggtg
    65941 atcgcgagca cccggggcgc gtagccccgg tcggccttca ggtcggccag ggcgcgcgcg
    66001 aacaattcga gctcgccctg caccagcaga ccgcgctcga cgaaccgcgc gagtggtgga
    66061 gcgatccggg catcggtcgg cgacaacccg ggactcttca gaaccagtcg aacgccttcg
    66121 aatgcggcat cggccagttc gccgcccagc acggtggcct gaggcacgtg ctctgcaagc
    66181 gcctgggcat tcggcgcaac ggcgcgggaa tcccagacgc gcacggttgc gccgcagcgc
    66241 gcgcaccagc gcgccatcgc cagacccgag tcgccgagtc cgagcacgag caccacgaga
    66301 ccctggaggt gattcatgtg cgtgccccgc tcatcgcagc ttcaggctgg ccagcccgac
    66361 caggcacaac agcatcgtga cgatccagaa gcgcacgacg acctgcgtct ccttccagcc
    66421 cgatttctcg aagtgatggt gcaggggcgc catcttcagg atgcgctggc cggtgccgta
    66481 gcgcttgcgg gtgtacttga accagctcac ctgcatcatg accgagagcg cctcgacgac
    66541 gaacacgccg cccatcacgc cgaggacgat ttcctgcctc gtgatcacgg cgatggtgcc
    66601 gagtgccccg cccagcgaga gcgcgccgac atcgcccatg aacacctgcg cgggataggt
    66661 gttgaaccac aggaaggcca gccccgcacc ggccatcgcg gcacagaaca ccagcaattc
    66721 gccggcgccc gggatgtggg ggaacagcag gtacttgctg aagtaggcat tgccgatgac
    66781 gtacgcaaag acgcccagcg ccgagcccac catcaccacc ggcatgatcg ccagtccgtc
    66841 gaggccgtcg gtcaggttga ccgcgttgct cgcgccgacg atcacgaacc aggtcagcac
    66901 gatgaagccg tagacgccca gcggatagct gatggtcttg aagaagggca cgatcaggtc
    66961 cgcagtcggc ggcagatcgt tcgagaagcc gctctgtacc cagcgaacga acagttcgag
    67021 gacccgcagg ttcgatgtct cgctgacgct gaacgccagg tagaacgcag ccaccagccc
    67081 gatcagcgac tgccagaaga acttgtctcg cgagctcatg ccctcggggt tcttgtcgac
    67141 gaccttgcgc cagtcatcga cccagccgat ggcgccgaat ccgagcgtca ccagcagcac
    67201 gatccagacg aagcggttgg accagtcgaa ccacagcagc gtcgacaccg tgatgccgat
    67261 caggatcagc acgccgccca tcgtcggcgt accggacttg ctgaggtgtt ccttgatgcc
    67321 gtactcgcgt atcggctggc cgatcttcag cgcagtcagc cggcggatca cccaggggcc
    67381 gaaggccagg ccgatcaaca gcgcggtcat cgcggccatc acagcgcgga aggtgatgta
    67441 ctggaagacc cggaagaagc ccagctctgg gtagatcgtc tgcatccact gcgcgaggct
    67501 aagcagcatg cgagcctccc gtcgacggcg cggcggcgct tttcagcgcg gcgacgatgc
    67561 gctccatctt catgaagcgc gatcccttga ccagcaccga tgtggcgacc ggagcctggg
    67621 gcagtgcggc cagcaggtcg tcgaccgaat cgaaatgccg cgccgcgcca aaggccgccg
    67681 cggcgtgcgc acacagggat ccggcggtcc acagcgcatc catgccggcg tcgcgcgcaa
    67741 acccgccgac ctcgcggtga aacgccggcc cctggtcgcc cacctcgccc atgtcgccga
    67801 gcaccagcca gcgcgggccg ggcaaccccg ccagcacctc gatcgctgcg cgcaccgaat
    67861 cgggattggc gttgtagctg tcgtcgatca gcgtgacctg acggccgtct cgcacgaagc
    67921 tgcctgcctg cgagcgcccc tggaccgcct tgaaggcttc gaggccgcgc actgccgcat
    67981 cgagcgggca tccggccgcc agagcgcagg ccgtggcagc aagcgcgttc ttgacgttgt
    68041 gcagtccggc gatgtgaagt ttcagccggc ggctgcccgc ggctgcatgc agcgccacca
    68101 gccagtgatc ggcggaccac gacgcagtcg cggagacatc ggccgcaacg tcgcccagcg
    68161 cgaaggtcag cacgcgcctg tcgcctgcca gcgcgcgcca cagcggcgtg aattcctcgt
    68221 cggccggaaa caccgcgacg ccatcggcgc ccagggcctc gatgacgctg ccgttctcgc
    68281 gcgccaccgc ttcgacgccg gacatgaact cctgatgctc gcgctgcgcg ttgttgacca
    68341 gagcgaccgt gggtgcggcc attgcagcca gctgcgcgat ttccccggga tgattcatgc
    68401 cgagctcgac aacgccggct ctatgaaagg tggtgtcatc ctgccgcagg cgcagcagcg
    68461 tcaatggcac gccgatgtcg ttgttgaagt tgccctcggt cgagaacgcg ccttcgccct
    68521 gccaggcccg aaggatcgcg gcgatcatct gcgtgaccgt ggtcttgccg ttgctgccgg
    68581 tgaccgcgat caacggaatg tcgaagccgc gccgccagcc tgccgccaac gcaccgagcg
    68641 ccaggcggct gtcgccgacc tgcagaccgg gcagaccggc ttgcgcgaca tcccgttcag
    68701 ccagcaccgc ggctgcaccg gccgcccggg cctggggcag gaagtcatgg gcatcgaagc
    68761 gctcgccgcg cagcgcgacg aagagatccc ccgcgcgcag gctgcgcgag tcggtgtgaa
    68821 cccgcttcag cggcgtggcg ccgtcgccga acaggcacga tcccggcagc agatcgtgtg
    68881 cgtgcgcaag cgtcatcacg gctgcttctc cgcaacgctt gcacgcgcct gcagttcggc
    68941 gcgggctgcg cttgcgtcgt cgaaaggatg actgacaccg gcgatgtcct gatgagcctc
    69001 gtgacccttg cctgcgatca ggaccacgtc gctcgaaccc gcccgagcca ccgcatgggc
    69061 gatcgctgca cggcggtcct cgatcacttg cggctggggc tgcccgccca tgcccgagac
    69121 gatcgccgag atgatcgagc ccggcacttc gtccctgggg ttgtcgctgg tcacgacgac
    69181 gtgccctgcc agccgctggg cgagcgcgcc catcagcggc cgcttgcccg catcgcgatt
    69241 tccaccgcag ccgaacacgc accacaacgc acctccgcgc gcggcagcca gcggctgcag
    69301 cgcgcgcaat gccttgtcga gcgcatcggg cgtgtgcgcg tagtcgacca gcacctcggg
    69361 cagttcctgg tcggcctgca accccagcgt gaccctctgc atgcggcccg gtaccggtgc
    69421 cagcgtcgag caggctcgcg ccgcatcgtg cagtgggacg cccagcgcgc gcagggaggc
    69481 gaccacctcg agcaggttgg agacgttgta gtcgccgatc agcgcggtgc gcaccggcac
    69541 ggacggttcg ccggactcga ccaggtcgaa tgccaatcca ccgtcgacgt agcgcaccga
    69601 tcgggcagcc aggcgcgccg aatcgcttgc cgctgccggg cccgccaagg cgcaggtcca
    69661 gacgtcgagc ccgctgccat cgagcgcctg ctgcagcgcc gcaccctgcg ggtcgtcgac
    69721 gttgagcacg acggcgcgca gtccgggcca gcggaacagg tcggccttgg cctgccagta
    69781 ggcatccatc gatccgtggt agtcgagatg gtcctgcgtg aaattggtga acaccgcgac
    69841 atcgatgtgc gtgcccgaca gtcgctgctc ggccagaccg atcgacgaag cctcgatcgc
    69901 acaggcgctg aacccgtcgt cggcaaagcg ccggaaggcc cgctgcaggg tgaccggatc
    69961 gggggtcgtc aagccggtcg cctgcaacgc cggcgcggcg gcagcaccag ggttgacggg
    70021 cgccgcaccg atgcccagcg tgccgatcac gccgcagcgc aggcccagca cggacaaggc
    70081 ctgcgccgtc caccacgcgg tcgaggtctt gccgttggtg ccggtgatgg cgatcacgga
    70141 cagccggcgg ctcggcgctc catagaacgc gcttgccagc acgccggtgg tggccttcag
    70201 gccgtccatc gcggcgaccc gcgcatcgtc gaagccgtag gcttcggctc cctcggcctc
    70261 gaccagacac gccggcgcgc cggctgcgag cgcgggctgg acatggcgcc gtccgtcctg
    70321 cgcctgaccg ggccaggcga tgaaagcgtc gccgggttcg agccggcgac tgtcggtggc
    70381 cagaccgcgc gcaccttgct gcgcgagcca ggacagcgca gccgggaccg agccgagtgt
    70441 ctgcagcgcc atcagaagct ctcgtcctca ccgggcacgt cgcgcgcgac gatctgcgtc
    70501 ttcacgtcga ggtcaggctg cacgccgagc gtgcgcagcg tgttctggac caggtcgctg
    70561 aagaccggcg ccgcgacgtc accgccgtac cactttccgg cggtcggctg gtcgaccatc
    70621 accgcgacga tgatgcgggg gttcttgatc ggcgcgaggc cgacgaacca cgagcggtac
    70681 ttcttgttgt cgtagctctt gccctcgtgg ctgtaggccg tacccgactt tccgccgacc
    70741 gagtagccga cggtctgcgc cttcggcgcc gtaccgccgg gcccggtgac cagcgcaagc
    70801 atctgccgca cggccagcgc cgtctgaggc gagatcacac gcaatccggg caccctggcc
    70861 tcggccccct gcgcctcgct cttcatcagc gtgacgggga tcatctcgcc gtcgtgcgcg
    70921 aacacggtgt aggcgtgagc gatctggaac agcgaggccg acagcccgta cccgtagcac
    70981 atcgtcgcgt gcatggtctc gtccttggcg atcgccttgg ccgagggcag ccgaccccgg
    71041 acggaccccg ggaagccgat ctgcggcttc tgcccgaagc ccaccgccgt gtacaggtcc
    71101 cacatctcgc gcggctggaa catcagtccc atgcgcatcg agccgatgtt gctggacttc
    71161 tggatcacct cggccaccgt caggtcgccg tgcgggtggg catcgctgat gatgaatcca
    71221 cccatcgcga tgcgcccggg cgcggtatgg atgatggtgt cgggcctgac ccggccggtc
    71281 tcgagcgccc aggccgcgat gaacggcttg accgtcgagc ccggttcgaa cgtgtcggtc
    71341 atcgcccggt tgcgcaactg ctcgccggtc aggttgcggc ggtccgacgg cgcgtagctc
    71401 gggtagttcg caagcgccag cacttcgccg gactgcgcat cgagaaccac cacgctgccg
    71461 gccttggcct tgttctcggc gaccgcatca cgcacccgcc ggtaggcgaa gtactggatc
    71521 ttcgagtcga tcgacaactg gatgtcacgg ccgtcgaccg gtgcgacgct gtcgccgatg
    71581 tcctcgacga ccttgccaag acggtccttg atgacgcgcc gcgtcccgtt gcgaccggtc
    71641 agctccttct ggaaagcgag ctccatgccc tcctggccgc gattttcggc atcggtgaag
    71701 cccaccacgt gtgcagcggc ctcgccctcc ggatacttgc gcttgacctc gcgcaactgg
    71761 tgcacgcccg gcagaccgag cgccagcgcg tccttggcct gcgtctcgtc gacctggcgc
    71821 ttcagccaga cgaaattggg gttgtcgtcg agccgatcgt cgagggcctt cggcgtcatg
    71881 cccaggaggc gtgcgagctg tcgacgctca cggctgtcgg ccgcgaagtc cttcgggatg
    71941 gcccacagcg agggcgccgg cacgctggat gccatcagtt gcccgtttcg gtcgaagatg
    72001 cgcccgcgat tcgccgacag gtcgagcgtg cgcgcatagc gggcctcgcc ctgcttcaga
    72061 tagaacgacg aaccgatgac ctggacgtac aacgcccggc cgagcagcac gcagaagccg
    72121 agaccgacca gcgcgaccag caccttggag cgggacgcgg gcgtgcgaga ggccagcagc
    72181 gggctgctcg cgtagttgat gctgcgcgca tggccggcca ccggcttggc gacggctcct
    72241 gcccgggcgt tccagccccc ctgcctcatg gctgcccccg cgacgacgcg gccagcccgg
    72301 gcccgacggc ggacgaggcg cgaccggcat ccgccgtgac cgtcacctgc ttcatgattg
    72361 ccggcgtggc ctgcaccatg cgcagcttgt cgcgcgcggt cttctcgacc ctcgcgggtg
    72421 cagcctgcgt ctgccgctcg gcctgcaggc gaccgtattc ggtctcgaac tggcgctgct
    72481 ggttgcgtgc gcgatcgagc tcgccgtaca ggtggtgcgc gtcgtaggag acacgcacca
    72541 gatagacgct gctgacgagc agcacgagga gcagcagcag gttgaggcgc gtcacgcgtg
    72601 cccccttcgc gatgcacgct gcaccaccca tcggcggcat gtgcaatccg gtcgcatcgc
    72661 cgacatcacc tgcgactccg ggccggcggc ggcggcgcag acgcggcgtc agtgcgctcg
    72721 gccacgcgca tcaccgccga gcgcgcgcgc gggttggcgc gcagctcggt ctcgccgggc
    72781 ttgatgcgac ccagcgccgt cagccgcatc gccacctcgg gcgcgaacgg tgcccggcga
    72841 tcgaacaccg ccttgctctc gcgcgcgatg aatgtcttga cgatgcggtc ttccagcgag
    72901 tggaaactga tcaccacgag acgaccgccg ggagccagca attcgagcgc cgcgctcagc
    72961 ccctgttcga gctcctcaag ctcggcgttg acgaaaatcc gaagagcttg aaacgtgcgc
    73021 gttgcagggt tctggcccgg ctcgcgggtc ttgactgcac cagccacgac ttcggaaagc
    73081 tcccgcgtgg ttcgaacagg gttcccgctt tcgcgccgag caacaagcgc ctttgcaacc
    73141 tgtacagcaa accgttcttc cccatagtcc cgaatcacct ccgcaatgtg gcgctcgtcg
    73201 gcgcgggcca gaaaatccgc agcgctttcg ccccgggtcg tgtccatccg catgtcgagc
    73261 ggaccgtcga aacgaaaact gaatccacgc tcggggctgt cgatctgcgg cgacgacacg
    73321 cccaggtcga gaagcagacc atcgatgcgc gagaccccgg ccgcggccag cgtcggcacc
    73381 atctggccga agctcgtgtg gtggatcgag aagcgcgggt cggcgaagtc cggatcgccg
    73441 gcggccgcag ccaccgcctc cggatcgcga tcgatcgcca gcaggcgcgc acgcgccccc
    73501 aggcgctgca gcagcaggcg tgaatgccca ccgcgcccga aggtgccatc gacatagacg
    73561 ccgtcggccg ctcgcgcaag ctgctcatcg tccgccgcac ccgggggagc ggcgtgcagc
    73621 agcgcatcga cggcttcgtc gagcaggacg gtggtgtggg cactggcagg tgcggtcgtc
    73681 atcgcacagc gctcagaaag tgaatccctt gagcgcttcg ggcatgtcgc ccttcaccac
    73741 ctcggcttcg tgcgaagcgt gctgcacggc atcccacagt tcgaggtggc tgcccatgcc
    73801 gatcaacatc acgtcgcggt cgagcccggc ggatttgcgc agttcggggg cgatgagcac
    73861 gcgcgacgag ccatcgagat cgacgtccat cgcattgccg agaaacatcc gcttccagcg
    73921 gaccgcctcc atcggcaacg cgttgatgcg gtcgcggaat tcctcccagg tcgggcgagg
    73981 aaacaccatc aacggaccgt cggggtgcct ggtgatcgtg agctcggtga cttccagtgc
    74041 gcgcaacacg gacaggtggc gggcaggcat ggtgagccgc cccttgctat ccaaggtcag
    74101 cgccgcaggc ccctgaaaca ccagatttgc cacaaaaacc cagaaaagtg caccaagtcc
    74161 cactgtatcg catcgcatct gcctgggtca acgcagatgt aaaagaaaat ccaattaaat
    74221 caatcactta gcgggcattc tgaaggcgcc gaacagccaa aatccttaaa aaacaagcag
    74281 ttgagaagcg ctttgaaagt gatgggtgag aagtcgccgg caggcatcga cgcgccagcc
    74341 ccgacaacga agctgggtca acgagcgccc gcccgccgtc gggcggcggc atgagtcgga
    74401 tcagcgcggg gacacgccga tcagccacag gtgcaccgca ccgatgaagg ctgcatacaa
    74461 acacaggcca acggtgagcg caatggcgtc gttgtagcgc ccgggcgggg ctccgggcac
    74521 gctgcgcgcg acgcggtgct tcagcgaaat ccggtcggcc acggcccacg ccaggaaggc
    74581 accgaacagc acggtggacg caagggtgcc gttggcgagc aggtgggcga gcgcccacag
    74641 cttggtagcg accagcatcg ggtgccgggt gatggccgag atgcgcccgg gcaggtacga
    74701 ggccagcagc agcggaaaca ccggcagcat cagcaaccac gccacatgcc gaagtgcggc
    74761 gggcggctgg tacagaagga ccggtgcgcc acgcgcctgc ccgtatccat gaaggatcag
    74821 cgcaaatccg accagcgaca ggagcgcgta gatgcccttc caggcgatgt cgccgagtgc
    74881 ggcagcctga cggttgcgcc aggccggcgc gaagatcgac gtcgagtgca caccgagaaa
    74941 gatgaccaga cccaggatca gcatggacat gtcgttcgcc ttcgcaaagc gatcagtcta
    75001 tgccgcgttc gcatcgcggg ccgacccgag ggcgacccgc tgcgggctca ggacatcagt
    75061 gcggcggcct gtgacagggg atcgtccttc gcgtcgctgt cgggttgcgc gttcggcgca
    75121 accgcagtga ccgtcgtcgt gcggggcatc gatcccagcc gggtgcgcgc aggtgcggga
    75181 ggcaccagcg gccggccctg cagccagtcc tcgatctgtg cgggcggcat cggcttgctg
    75241 aacaagtagc cttgaagttc gtcgcagcct gcatcgcgca gccagttcgc ctcttcaaga
    75301 cgctcgacgc cttcggcgac cacgcgcaga ccgagcgtgt ggccgagccc gatgatggcc
    75361 ttggtgatgg cccagtcggc cggatcttca aggatgtcgc gcacgaagct ctgatcgatc
    75421 ttcagcttgt cgatcgggaa ctggtgcagg tagttcaggc tggagtagcc ggtaccgaag
    75481 tcatcgatcg acaaggtcac gccgatgtcc ttcagccgtt ccaggctctc gaccgtcgcg
    75541 atcgcgttct ccatcaggaa ggtctcggtc agctcgatct cgatggccga gggatcgacg
    75601 ccgtgcgtgc cgagcgcttc gcgcaattgc gctggcaggg acgagtcccg cagctggatg
    75661 gccgacacgt tgaccgagat cgggatcacg ccgagaccgg catcgcgcca ggccgcgtgc
    75721 tggcggcagg cctcgccgag gatccacgca ccgatcggca cgatcaggcc ggtctcttcg
    75781 gcgatcggaa tgaaagtcgc cggcgagacc atgccgtgct ccggacgctg ccagcgcacc
    75841 agggcctcaa ggccgacgac tccgccgctc gctgcgtcga cccgcggctg gaaatgcagc
    75901 agcaactcgc ccctcgcggt gacctcgcgc agctcgttct cgatcgcgag gcgctcctgc
    75961 gcctggatat ggaactcggg ggtgaagaag agcgcctgat tgcggccgcc cgacttggcc
    76021 cggtacatgg cggcatcggc gtggcgcatc aggtggtcga tgtcgcgacc gtcctgcggg
    76081 tagacagcca tgccggcgct gcacgacaca tggagctgga cgccttcgac ggtgtgggct
    76141 tcgcgcacca ggggcagcat gcgctcatgg acgatgctgg cgacctcttc ggcgctgctc
    76201 acgccattga gcaccacgac gaattcgtcg ccgccaaggc ggctcaccgt gtctcccgcg
    76261 cgcaccgcct ggatcagtcg cctggatacc gagcgcagca gggcatcgcc gacatggtgc
    76321 cccatcgagt cgttgatgtt cttgaaacga tcgaggtcga tgaagacgac ccccacatgc
    76381 tgaccggttc ggccggcctg ttcgaccgcc atgcgcagcc gctcgaggca cagcgaccgg
    76441 ttgggcagat cggtcagcac gtcgtggtgg gcgaggtggt ggatgcgctc ctcgctcgcc
    76501 ttgtgttcgg tgatgtcggc acccgctgca acgaaatgcg tgatccggcc atcgctgtcg
    76561 cgcaccgcgt tggcaaccag ccaggtcgga taggcctcac ctgtcttgcg cttgagccag
    76621 acctcgccct gccacatgcc gcggatgaca gccgactgcc agagcgtctc gtagaaatcg
    76681 gcatcgtgtc gcgacgagta gaggaattcg ggcgtctgcc ccaccacttc ttcagcgccc
    76741 caacccgaat cgcggctgaa ggccgtgttg gcggtcagga tgcgacgttc gacgtcgaag
    76801 atcacgatgc tttctgatga ggcctcgaac accttggccc acagcttcag gcgctgctcg
    76861 agcatcttga tctggccgat cggggtgaag accgtcagca acgccggctg cccctggtag
    76921 gtcaggcgcc tggcggaaag caacgcccac gaggcgcggg agccaccagc actggcttcg
    76981 cccgactgcc actgcacctc gaagccatcg acacggtcgg tgtcgctcag acgcgagaag
    77041 aaacgggcgc gagcatgcgg ttcgaggcgc aggctccatg gatcggtcgt cacgccctgg
    77101 agccagggct gcgccggctg gttggcatga agcacgcggt gctccgggat cgaggtcacg
    77161 atcaacggca tcgggaagga ttcgagcagc gcacgctgag cttcctcggc gcggctggtg
    77221 gcggccaggt cttcgcgcac gacgcgctcg cggtccaggt cgccaagcat gccgttgaag
    77281 gcctgcacca gttgcccgat ctcgtcgcga ctttcatggg cggcgcgcat cgtgtagtcg
    77341 ccggtggcgc tgacgcgccc ggcgacatcg gccagccgct gcaggggctg cgcgatctgg
    77401 cgagccacga agtagacgat gctgaggatc agcatcaaca gtgccacggc cgtgcccagg
    77461 tgcgtccaca ttcgcacgaa cagtccctgg atgcgggcat cgagcagacc ttcgagcccc
    77521 acggtggccg cgcgccaggt tgcgtgcagt tcggccacca gttcgcggtg cctggcggca
    77581 agcacttcac gacggtcggc ggcaccgcgg tcagcgatgt gcatcgggtg ctgacggaag
    77641 gacgccaccg ctgccgtgag gcgatctcgc gccggcgcca gcgccgcgcg cagcgtcggc
    77701 gggccagccg ccagcgcttc cgcatagtcg gaatcgatgc ccttgagcac ggcatcgagc
    77761 cggccctcga ggatcagcaa ttcggtgtgc accttgttgc gctggtcggc cggactctga
    77821 tccagttcga ccgccttgtg caccgtcagc gcgagcagtt cctgcagctc aggaaaccgc
    77881 agcagcacca gcgacatcgt gtaatagctg tcgagatcgg gatcgaggat caggttcgac
    77941 tgattgccga tgcgggtgat cagttcacgg ccggcagcca tcgaggccgc cagttgtcct
    78001 gcatcgaaga cgggccggtt catcgccgtc tgcaacgcag ctgcgaaggc ggtactgaca
    78061 tcggcggact tcatgccctg gccgtggcgt gactcggcct cgcgaagcgc ggcgagccgc
    78121 ggctcccact cgacccggtc gagtcgcgtc gcggccgcat ccgcgccggg atcgcccgcc
    78181 ttggcgggcg gcaagggtgt gggcagcgcg accagcacat cgcggaccgc ggcgatgtag
    78241 gcgttgccgg cgacttcctt gcgcgagaaa tcgatcgata tgtatttttc gctgatcagg
    78301 atgctcgaga cgtagatgac cgccgtgagg tcaagcaggt agatcagcag gagcttgcgc
    78361 ccgacgggca gctgtccgat cgcccggatg atcagggtca acatgtctca ataccctctg
    78421 gttgcgaacc aggacatcgc aagcaagagc gcggccacaa aatcaactgg acccgaaaca
    78481 ccgcgctgtt cgacggctcg agggatcaat tcgattcctt gatgatccgg ccgctggccg
    78541 actggatcgg gcgggcattc atcggataga gccgcgagaa gatgctcagc tgttcggggg
    78601 agatctcgac cggcgtcttc atcaccatcc agagcacgcc ctcgctgcag ggcggcgtgg
    78661 tcagcgatcc catgtaggtg aagtagcgcc gatcggtcgg cagcagtgcc atcaggtcga
    78721 tctggccctt ggccatcacc tcgtctccct tttcgagcgg caggttgttc cacacgttct
    78781 gcacgaccgg ctgggcactg ccccggtcga gcagcacggc gatcaccgcc agcttgccct
    78841 cgacgccctt gtgcaccaga tgcaccacca tgtcgaacgg cttgccgttg atgcgctcct
    78901 cggaagggcg atggaagtgg aactggacca gctcgtaacg cttgccggtg acctcgatcg
    78961 tgttgcccac cgccacgttg acctgcaccg tgtgtccgtt gtctatgacg cggaagttgc
    79021 tcggccggta gttgaactgc accgcatcga gctcgacctt gatgccatcg gtgatgtcga
    79081 tcgggctttg gcgggtgccg gtggagcacg tcgcgaactc gggctggagc gcaccccagg
    79141 attcggggcc ccccgcacct tcgtagctcc agtgcgcagc ggactggtga cctgcagccg
    79201 ccgccttgag ttgcgcggct tcgagcctgg ccgcatgggt cgggctggcg ccgatcacgc
    79261 gagtggagtt cgacaccggc gtcagcatgg cgcgggacac cggcgctgcc ggcgcagcgg
    79321 cggaatgcga gaccaccttg accacgccag gcgtggcgga ctcgctcacc ttcgatgtac
    79381 cgagcttctc ggcgagtcgc tcgcgaatcg catccatcgg gtcgcgtgga ggacgcttca
    79441 ccggggtgtc tgtgctggtg tgctccgagt cggatgccga agcgg
//
LOCUS       DAOORV010000002        69720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_402_length_69720_cov_8.021532, whole genome shotgun sequence.
ACCESSION   DAOORV010000002 DAOORV010000000
VERSION     DAOORV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 69720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_69720_cov_8.021532"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PKV56_00370"
     CDS             complement(<1..823)
                     /locus_tag="PKV56_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOX66286.1"
                     /translation="MSEPDDLVNHMHRVLAGERDLRDLGMLWQMIESAAAISCPEEVA
                     PILPTLISTRERFNDLQGRLIGRMVTEQRAALGEELTAAAQCAIDTLVRNLYERTADV
                     GFLATDDVVRAFCQGTADDQQASRDSMVHRLFEYQAKYTVYDDVILLSPAGVVLARLD
                     REAPLAISSDAIVADALAAHAYVERFGPSDLGRDQSPALLYAHRINGSGGRPAGVLVL
                     RFRFEDEMSRIFNGGDGQVPQEIALVLLADNGRVIATNDHGHVQNGVTLPSMKNGQ"
     gene            1051..1986
                     /gene="argB"
                     /locus_tag="PKV56_00375"
     CDS             1051..1986
                     /gene="argB"
                     /locus_tag="PKV56_00375"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOX66287.1"
                     /translation="MNLPKPVSASATASPAARPAEIAPRDKAEILAQALPYIRKFHGK
                     TIVIKYGGNAMTDPELQQDFAEDVVLLKLVGMNPVVVHGGGPQIEDALAKLGKKGTFI
                     QGMRVTDEETMEVVEWVLAGQVQQDIVGLINVAGGKAVGLTGRDGGLIRARKLRMQDL
                     KDPTVEHDVGSVGEIESIDPSVVKALQDDQFIPVISPLGFGANNENYNINADVVAGKL
                     AEVLKAEKLMMLTNIPGVLDKNGKLLTNLTAREIDDLFADGTISGGMLPKIASALDAA
                     KNGVNTVHIIDGRVPHAMLLEVLTDQAYGTMIRSH"
     gene            2054..2767
                     /locus_tag="PKV56_00380"
     CDS             2054..2767
                     /locus_tag="PKV56_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOX66288.1"
                     /translation="MSTGRARRVWLFDLDNTLHDASHSSFGPTNEAMTQYIVDHLDLP
                     HPEAWALRQHYWLHYGATMLGLVRHHGVKASHFLEATHRLPGLESRLRTSAHDRAALR
                     RLPGRKFILTNAPRAYALRVLDALRLSGCFDGLISIEDMTMFGHHRPKPDARMFRSMA
                     ARLGVPPHRCTLVEDTLDHQRAAYRVGMRTVWMQRYIPRVRRLSAPPESASEVSVHPC
                     HKPLYVCARIKSIQGLLSM"
     gene            2793..3470
                     /gene="slmA"
                     /locus_tag="PKV56_00385"
     CDS             2793..3470
                     /gene="slmA"
                     /locus_tag="PKV56_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOX66289.1"
                     /translation="MTTRRDDTLDSDGEETPPAVDAASESPAAGTSRKRPKPGERRVQ
                     ILQTLAGMLEQPGSERVTTAALAARLGVSEAALYRHFASKAQMFEGLIEFIETSFFSL
                     ANQISEREPSGVQQARKVVTMLLQFGERNPGMARVMVGDALVFEHDRLVMRMSQFFDR
                     VESQLKQSLRVAGDASGSVMATVDANAVASVLTSFSVGRLQRYARSDFKRPPTEHLDA
                     ALNLILA"
     gene            3642..4523
                     /locus_tag="PKV56_00390"
     CDS             3642..4523
                     /locus_tag="PKV56_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66290.1"
                     /translation="MSTLPDPLLHLLHRAEALISRIEAVLPHPLTAPDWAASIAFRYR
                     RRSAGGSALEPVRHVAPIRLADLVEVEPQKERLVRNTEQFVEGRTANNVLLTGARGTG
                     KSSLIKACLNEFAPRGLRLIEVDKADLVDLTDIVDLVAERPERFIVYCDDLSFDEGEP
                     GYKALKSILDGTVSQASDNVVIYATSNRRHLLPEYMAENLTYQHLENGEVHPGEVVEE
                     KISLSERFGLWVSFYPFTQDEYLAIVSQWLRGFGVGESAIEAARPESLVWALERGSRS
                     GRVAYQFAKDFSGRLPG"
     gene            4520..4951
                     /locus_tag="PKV56_00395"
     CDS             4520..4951
                     /locus_tag="PKV56_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOX66291.1"
                     /translation="MSAEHRTPVDVAVGVLIDAQGRFLMTSRPEGKVYAGYWEFPGGK
                     LEAGESVEEALRRELHEEIGIVIGEAHPWQVELVDYPHALVRLHFCRVYDWRGEFEMR
                     EAQTMSWQTLPVEVMPVLPGTIPVLRWFAEERQHAGATHRG"
     gene            4994..5383
                     /gene="hisI"
                     /locus_tag="PKV56_00400"
     CDS             4994..5383
                     /gene="hisI"
                     /locus_tag="PKV56_00400"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOX66292.1"
                     /translation="MSWIDDVKWDAQGLVPVIAQEIGSNDVLMFAFMNREALQRTADL
                     GEAVYWSRSRSRLWHKGEESGHIQKVHELRLDCDNDVVLIRVEQVGHSPGIACHTGRH
                     SCFYQLYRDGAWHTVEPVLKDPEHIYK"
     gene            5380..5751
                     /locus_tag="PKV56_00405"
     CDS             5380..5751
                     /locus_tag="PKV56_00405"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOX66293.1"
                     /translation="MTSPDATLPLSQDVLARLAEVIESRKAGDPDKSYVARLFHKGTD
                     SILKKVGEEATEVVMAAKDGDPQKVIYEVADLWFHSMLALSAFGLKPADVLAELQRRE
                     GLSGLEEFAARKPIQREGGEK"
     gene            5748..6116
                     /locus_tag="PKV56_00410"
     CDS             5748..6116
                     /locus_tag="PKV56_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66294.1"
                     /translation="MTEAVLAGPEEKPHGGLRTLTHILYGLHLLSWFSAGAFSLVAMV
                     INYIKRPDLPDELFRSHFRWQARSFWFTLLWLTVTSPLFLLFWIPGAAAWFLIGLWYL
                     YRFVRGWLSFAESRSMPQPS"
     gene            6135..6482
                     /locus_tag="PKV56_00415"
     CDS             6135..6482
                     /locus_tag="PKV56_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOX66295.1"
                     /translation="MNADPNCIFCKIVAGQIPSRKAFEDEDVLVFHDIHPWAPVHLLI
                     IPKEHIATHADVTPEHERLLGKMLLLVPKLAKEHGADNGFRTIINTGRDGGQEVLHLH
                     MHVIGGPRPWKQG"
     gene            6518..6754
                     /gene="tatA"
                     /locus_tag="PKV56_00420"
     CDS             6518..6754
                     /gene="tatA"
                     /locus_tag="PKV56_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOX66296.1"
                     /translation="MGSFSIWHWLIVLVIVVLVFGTKKLKNIGADLGGAVKGFKDGVK
                     DGGASADATAPAQQVTSQKSADANTVDVEAKTKS"
     gene            6751..7248
                     /gene="tatB"
                     /locus_tag="PKV56_00425"
     CDS             6751..7248
                     /gene="tatB"
                     /locus_tag="PKV56_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOX66297.1"
                     /translation="MIDFGFDKLALIGAVALMVIGPERLPKVARTVGHLIGKMQRYVA
                     DVKSEVNRSIELDELKKMKGQFEDAARNVEQTVSNEIHQTNSDLRDSVEADRGTAVDT
                     TSPDFNRYDTLLTPLPDYRNPKKQWRLKRTAMPQWYKQRHGIRGKALSGSARVARFRP
                     PRSSA"
     gene            7252..8037
                     /gene="tatC"
                     /locus_tag="PKV56_00430"
     CDS             7252..8037
                     /gene="tatC"
                     /locus_tag="PKV56_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOX66298.1"
                     /translation="MASNPSGPDGLGGEQPFVTHLIELRDRLVRASIAVGIAFGVLCL
                     WPGPGGLYDLLAAPLVASLPKGATLIATNVISPFVVPLKITLMAAFMLALPVVLYQVW
                     AFVAPGLYTHEKKLVLPLVVSSTLLFLVGVAFCYYFVFGQVFKFIQSFAPKSITAAPD
                     IEAYLSFVLTMFIAFGAAFEVPVVVVVLARMGLVTIEKLKEFRSYFVVLAFIIAAIIT
                     PPDVVSQLALAIPMCLLYELGIWAARIFIKHTQAPVEKATPTT"
     gene            complement(8041..9207)
                     /locus_tag="PKV56_00435"
     CDS             complement(8041..9207)
                     /locus_tag="PKV56_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX66299.1"
                     /translation="MRKTWLIFSQSVTVAVAVLFVIATLKPEWLRRHSIAGLPDMVSI
                     SEAPAASSASSHANSYAAAAKRASPAVVSITASKTSTRTPHAGDPWFEFFFGDRDNRQ
                     ETQIGLGSGVIVSPEGFLLTNNHVIEGASDIDVQLPDGRSAPATVVGTDPESDVAVLK
                     IALDRLPVMTFANTDALQVGDVVLAIGNPFGVGQTVTSGIVSALGRDHLGINTFENFI
                     QTDAAINPGNSGGALVDANGNLLGINTAIYSRSGGNMGIGFAIPANIARQVMEGLIQD
                     GQVTRGWIGVEPRDLTPEIAETLNLSVKQGVLITGVLQNGPASEGGMLPGDVVISVGD
                     NRVLNTAQLLNAVAALKPQAATSISVQRGNQVLSLKITAAQRPKNPASTAARGR"
     gene            9220..9969
                     /locus_tag="PKV56_00440"
     CDS             9220..9969
                     /locus_tag="PKV56_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOX66300.1"
                     /translation="MARRSEIDSYLSALLQTASFKDYGPNGLQVEGRDEVRRIVSGVT
                     ASRALIDAAIDARADALLVHHGLFWRGHDGRVTGWLKQRLERLLAHDINLFAYHLPLD
                     AHAEFGNNAQFGARLGVVADARFGEQSLGFIGQLAEPTSVAVLVDRMLRLSGRAPVSI
                     DGDGRPLARLAWCTGGAQSYYEAAIAAGADVFITGELSEPQAHLARETGVAFIACGHH
                     ASERYGAPAVGGHLAAQFGIEHIYIEIDNPA"
     gene            10022..11062
                     /gene="pdxA"
                     /locus_tag="PKV56_00445"
     CDS             10022..11062
                     /gene="pdxA"
                     /locus_tag="PKV56_00445"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOX66301.1"
                     /translation="MRERAQHAATPIVITMGDACGIGPEIIAKLFRGPSAQGCFVLGD
                     VEVMRRAARLTGGLLALAPIDSPDALADVPPNCVPVLQADALPHDLLSAPIGRIDSRA
                     GAAAARCIEQAVALALRGEVSAIVTSPIHKEALAAAGVGFPGHTEMLQALAAEPGMPP
                     PPVRMMLANDELRTVLVTIHQSLRSAIDAVTFDAVLDTVRIAHRAAVRWGLSSPRIAV
                     AGLNPHAGEGGLFGDEETRIIAPAISAARAEGIDASGPYAPDTVFMRARNALPAHPGE
                     FDVVVAMTHDHGLIPVKYLGVEHGVNVTLGLSFVRTSPDHGTAFDIAGSGVADPSSLE
                     AAIAMARKLAKD"
     gene            complement(11114..13723)
                     /locus_tag="PKV56_00450"
     CDS             complement(11114..13723)
                     /locus_tag="PKV56_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOX66302.1"
                     /translation="MNPTDQRLPAELEAAADLVKATALTVAERVCQTLAAQSQSASKA
                     IDRNTMFAAQLALHRQLGPFRLNFRTRLDARMLETLAPRTETRRHAHAADWETLTLVD
                     DKEVEERMHSDRLGQQIAHACEVELRELSGYMGRITGFGTADDEHNPLRPSIVGQALY
                     FAIEAVTSDPTELALLAREFGIVMAQAMPACYAAIVVEMQSRGISPVSLQVRTVEGPG
                     NALPGGINSGYASLRDELSSTRGHGSEFQTSSHSGLSAHRSTSGPTTSGSRSGSSSGY
                     GSTSWQGTRSGRIDAAPSGHGSGSRGPTTGYGGPGHVSGGGAAPAQDAQLMTLLRRLT
                     QLSNSSDELGLPSGLHGSTSPSAPASRYAAEPALRGIATGPYADPGPASAYADDASAG
                     LMAVNLIRAHREELIQASTGKLDHMVIDVVGSLFDQILSDTRVPPQMAREIARLQLPV
                     LRVALVDSTFFSSRRHPVRRFINRIAALACAFDDFDDGPGKEFVERVRDLVQEIVEGD
                     FDQVDLYAAKLGELESFITQQATSEVKDRGAIAVLENKETDLRTQQRYLIQLQGALSS
                     ISMPDFLRSFLCQVWSQALVQAARKQGDDGEPTKRYRNVGRDLVMSVQPKGSPGMRKR
                     FLMQLPTLMRDLNEGLKLIGWPEGAQKEFFGQLLPAHAESLKLPAMTELDYNMLSKQL
                     ESIFNSGVPEKGDSLRTDPGLSVPLTSAEIEQRFTPEEAQQVGLIDEKTVDWAQQVDI
                     DLSHEPPPTLESAPLDLGVDINLDLVGADPAEASHGARLIDHLRIGFAYQMLLKDQWQ
                     KVRLTYMSPGRSFFVFTHGRNLQETISLTARMLSRMCEAERLRAVESNYLMERATSRA
                     RKQLSALTANSRQ"
     gene            complement(13835..14254)
                     /gene="mscL"
                     /locus_tag="PKV56_00455"
     CDS             complement(13835..14254)
                     /gene="mscL"
                     /locus_tag="PKV56_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOX66303.1"
                     /translation="MGFASEFKEFAMKGSVVDLAVGVIIGGAFGKIVDSLVSDVIMPV
                     VSKIFGGLDFANYFIPLSGQAATTLADAKKEGAVLAYGSFFTVAINFVILAFIIFLMV
                     KAVNRMKQQQPEAPAAAPAAPPEDVLLLREIRDALRK"
     gene            14501..15100
                     /gene="petA"
                     /locus_tag="PKV56_00460"
     CDS             14501..15100
                     /gene="petA"
                     /locus_tag="PKV56_00460"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOX66304.1"
                     /translation="MSEQQVDSGKRTWLIASGCAGAVGGVAVAVPFVSTFQPSERARA
                     AGAAVEVDISALKPGEKLTVEWRGKPVWIVKRTPEQLAALSKVDSQLADPQSLRKPGE
                     LTPEYARNEHRSIKPEIMVAVGICTHLGCSPSDKFTPGAQPSLPDDWVGGFLCPCHGS
                     TFDLAGRVYKNKPAPDNLEVPPYMYLSDTRLLIGEDKKA"
     gene            15137..16537
                     /locus_tag="PKV56_00465"
     CDS             15137..16537
                     /locus_tag="PKV56_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HOX66305.1"
                     /translation="MATFKEAPANANAAEKLLTWVDNRFPATKLYNEHLADYYAPKNF
                     NFWYFFGSLALLVLVIQIVTGIFLVMHYKPDAALAFASVEYIMRDVPWGWLVRYMHST
                     GASAFFVVVYLHMFRGMIYGSYRKPRELVWIFGCAIFLCLMAEAFMGYLLPWGQMSYW
                     GAQVIVNLFSAIPFIGPDLSLLIRGDYVVSDATLNRFFSFHVIAVPLVLLGLVVAHVI
                     ALHEVGSNNPDGVEIKSKMNAAGKPLDGIPFHPYYTVHDVLGVSVFLLIFSAIIFFAP
                     ELGGYFLEYNNFIPADSLKTPLHIAPVWYFTPYYSILRAITAEMMYVLIGFVVLGAAL
                     AVFKVKMGALLKLVVVAVAAAVVAMMLSIDAKFWGVVGMGGAVVILFVLPWLDNSLAK
                     SIRYRPDWHKYVFALFVINFLMLGYLGTQPVSPTGERLSQVGTVIYFGFFLLMPWWSR
                     LGEFKPVPDRVTFAAH"
     gene            16560..17330
                     /locus_tag="PKV56_00470"
     CDS             16560..17330
                     /locus_tag="PKV56_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HOX66306.1"
                     /translation="MKKFIITLLLSVAGLSVANANTGGIAWDKFPVEKAADVTALQNG
                     ARTFVNYCLNCHNAAFMRYNRLRDIGLTEDQIKKNLMFATDKVGDVMKVTLDPKNAKD
                     WLGAVPPDLTLITRSRSAAGQGTGADYVYTFLRTFYRDETKATGWNNLAFPNIGMPHI
                     LWEWQGQRSAKFVEEADPHDQTKKVHRFAGFEQITPGTKSEAEFDQAVGDLVAYLNWM
                     AEPAQGTRTRLGVWVLLFLAVFTFIAWRLNAAFWKDIK"
     gene            17443..18054
                     /locus_tag="PKV56_00475"
     CDS             17443..18054
                     /locus_tag="PKV56_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOX66307.1"
                     /translation="MMVLYSGTTCPYSHRCRFVLFEKGMDFEIRDVDLFAKPEDIALM
                     NPYNEVPILVERDLILYESHIINEYIDERFPHPQLMPGDPVARARVRLFLLNFEKELF
                     AHVNLLESRGVKATDKQLEKARAQIRDRLTQLAPIFLKNKFMLGDDFSMLDVAIAPLL
                     WRLDYYGIDMSKNAVPLLKYAERIFSRPAYIEALTPSEKVMRK"
     gene            18089..18640
                     /locus_tag="PKV56_00480"
     CDS             18089..18640
                     /locus_tag="PKV56_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HOX66308.1"
                     /translation="MTLPPTPPVLGNSGTSTRPYLIRALHEWCTDNGFTPYIAVFVGP
                     DVKVPMEYVKNGEIVLNVGFEATTALELSNERIEFKARFGGTSRDISVPVDHVIAIYA
                     RENGQGMAFPVPADAAAPAGSGGGPRVQPQEPAERVGMVLRLAAEGDEPAAVPAAAPA
                     PAGDDPPSEPPPAGGRPALKRVK"
     gene            18684..18759
                     /locus_tag="PKV56_00485"
     tRNA            18684..18759
                     /locus_tag="PKV56_00485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18717..18719,aa:Thr,seq:tgt)
     gene            complement(18816..19130)
                     /locus_tag="PKV56_00490"
     CDS             complement(18816..19130)
                     /locus_tag="PKV56_00490"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOX66309.1"
                     /translation="MSKAAPSVPDALTLGEALRRSNPLASLRRRLDESARRFAAIQSC
                     IPPGLMAHVAPGPIDDEGWTLIAANANVAAKLRHLQPRLEEQLLRRGFPSLAVRIKVR
                     AA"
     gene            19366..22170
                     /gene="secA"
                     /locus_tag="PKV56_00495"
     CDS             19366..22170
                     /gene="secA"
                     /locus_tag="PKV56_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOX66310.1"
                     /translation="MLPKILTQIFGSRNERLLKQYRRVVVQVNALEPKFEALDDAALA
                     AKTVEFRQRHVAGESLAALLPEAFAVVREGSKRSLKMRHFDVQLIGGMTLHDGKIAEM
                     RTGEGKTLMATLPVYLNAIANKGVHVVTVNDYLAKRDAEWMGRLYNFLGLSVGVNLPQ
                     MQREEKQAAYAADVTYGTNNEFGFDYLRDNMVQEVSDRVQRQLSYAIVDEVDSILIDE
                     ARTPLIISGQAEDHTEMYVRINDVVPKLKKQIGEADPRTGEGVIEPGDFTVDEKTHQV
                     YLTEDGHENAERLLSEAGLLAEGASLYDAANISLMHHVYAALRANHLYNRDQHYVVQN
                     GQNGPEVVIVDEFTGRLMTGRRWSDGLHQAVEAKEGVPIQSENQTLASITFQNYFRMY
                     GKLSGMTGTADTEAYEFQEIYGLETVVIPPNRPTVRKDELDLIYKSSREKFEAVIADI
                     RECHERGQPVLVGTTSIENSELISGLLQKAGLPHQVLNAKQHAREADIVAQAGRPGVI
                     TIATNMAGRGTDIVLGGNVENQIKSLQADPTIPEAEQLSQARKLHEEWQGLHDRVKAE
                     GGLRIIATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDSLMRIFAGDRVRSIMER
                     LKMPDGEAIEAGIVTRSIEGAQRKVEARNFDVRKQLLEYDDVANDQRKVIYQQRNEIL
                     EAQSLMEQIGSLRRSTMADVVRTFVPAESLEEQWDLDALEKVLREEWQLELPLKAEVE
                     ASASITDDDIVERVAKAADQLFDAKVEMVGVDQFTPFIRMIFLQSIDQHWREHLAALD
                     YLRQGIHLRGYAQKNPKQEYKREAFELFSQLLDVIKMDVTRTLMAVRIRSAEEVAQAA
                     EEIEQRAERIANVTYTHPNEDGSLSTESQQAMTDPAMQVAQEMGERAEAPASFARAGR
                     NDPCPCGSGKKFKLCHGKLA"
     gene            22181..23422
                     /gene="argJ"
                     /locus_tag="PKV56_00500"
     CDS             22181..23422
                     /gene="argJ"
                     /locus_tag="PKV56_00500"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOX66311.1"
                     /translation="MPVNLKAPNPQDLKPVPGVRLGTAMAGVRKANRRDVLVVTLAEG
                     SQVAGVFTSNRFCAAPVQVCRQHLAVDRRAAAGIRALLVNTGNANAGTGEDGLARARS
                     TCVALSGRLGLQPDQVLPFSTGVIMETLPNDRIEAGLPAALADLDADHWAAAAEAIMT
                     TDTLPKAASVQVQIGGRTITVTGISKGAGMIRPNMATMLGFVATDAVLAPGLLDTLVR
                     EAADCSFNRITIDGDTSTNDSFLLIATQQGGHPPITQLDSGDGAVLRDAVIAVSTTLA
                     QAIVRDGEGATKFIAVQVDGGATEAECRLAAYAIAHSPLVKTAFFASDPNLGRILAAV
                     GYAGIADLDQTLIDLHLDDVHVVHNGGRHPAYREEDGQRVMKQSEITVRVNLHRGAAS
                     TTVWTCDLSYDYVKINADYRS"
     gene            complement(23410..24216)
                     /gene="ugpQ"
                     /locus_tag="PKV56_00505"
     CDS             complement(23410..24216)
                     /gene="ugpQ"
                     /locus_tag="PKV56_00505"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF006989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOX66312.1"
                     /translation="MTTDSCSLDAIAAWQSTRWLAHRGAGRLAPENTLAAFRAGARFG
                     YTAFECDVKLSSDGVAFLMHDATLDRTTGTTGIAGDRTWAELSTLDAGRWHSAPFEGE
                     SIPRLATIARFCIDSGSLLNIEIKPTPGLEALTGARVASEAARLWVGQRRLPLLSSFS
                     ADALAAAREAAPRLPRALLLDTLTPGWFEQARALACKAVVARHPLLDAALIERAHAEG
                     WLVLAYTVNDAALADWLFKCGVDALITDAVDRLPPRQATGEIESAPDQLR"
     gene            24404..28345
                     /locus_tag="PKV56_00510"
     CDS             24404..28345
                     /locus_tag="PKV56_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOX66313.1"
                     /translation="MNAPTTLTELTSAARVETGTPRLREIPYNYTSFSDREIVLRLLG
                     SRAWELLGRLRGERQTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNPGRRSLLIEALH
                     HRLKEIENRATPSDGHHHGDVSELLACAKAAVRRFADQFEEVASLRRRATRTLRRCTA
                     ADNVKFDALSRVSHVTDATDWRVEYPFVVLTPDTEAEMAALVKGCVELGLTIVPRGGG
                     TGYTGGAIPLTWNSAVINTEKLEAMSEVEMRELPGVDRPVATVWSEAGVVTQRVADAA
                     ERGGFVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPEAKWLEVT
                     RLNHNLGKIHDVEVASFELKYFDASGKTLERTERLDIPGGTFRKEGLGKDVTDKFLAG
                     LPGVQKEGCDGLITSARWIVHKMPAHVRTVCLEFFGNAKDAVPSIVEIKDYLFAQAKA
                     GGAVLAGLEHLDDRYLKAVGYATKSKRAHAGGNTSGLPKMVLFGDIAGNDADAVARAT
                     SEVIRIANSRAGEGFVAVSPEARKKFWLDRKRTAAISKHTNAFKVNEDVVIPLGRMGE
                     YTDGIERINIELSLRNKLALLDALEEFFRKGQLPLGKGDDAVDIPSAELLEDRVQQAL
                     SVVEEVRALWQHWMTHLDISDGERSYFAPLQDRTLRASWKLQVRAPLQAIFAGAALAP
                     IVEACQKIHKDVLHGRVWIALHMHAGDGNVHTNIPVNSDDYDMLQTAHEAVARIMNLA
                     RRLDGVISGEHGIGITKLEFMTDAELADFAAYKQRVDPEGRFNRGKLLRGGLGRSGAV
                     DVGTSSEVRPSDLSQAYTPSFGLMGHESLIMQQSDIGAIADSVKDCLRCGKCKPVCAT
                     HVPRANLLYSPRNKILATSLLIEAFLYEEQTRRGISLRHWEEFEDVADHCTVCHKCLS
                     PCPVKIDFGEVSMNMRNLLRKMGKKSFRPGNAVAMTFLNATSPQTINLMRGAMVGIGF
                     KAQRLANDLLQRLARKQTALPPATVGTAPLKEQVIHFINKKLPGGLPKKTARALLDIE
                     DKDYVPIIRNPQTTTSETEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYL
                     CCGYPQRGSGQFDKADKIITDNRVLFHRVANTLNYLDIKTVVVSCGTCYDQLQGYQFD
                     KIFPGCRIIDIHEYLLEKNITLPGQGGYLYHDPCHSPMKLQEPMKTVKALLGANVIDA
                     KRCCGESGTLGVTRPDISTQIRFRKEEELRKDEAALRRTGAVAETGAIKVLTSCPSCL
                     QGLSRYTGDLQNGLLEADYIVVEMANQLLGPDWMPQYVERANQGGIERVLV"
     gene            28345..28782
                     /locus_tag="PKV56_00515"
     CDS             28345..28782
                     /locus_tag="PKV56_00515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOX66314.1"
                     /translation="MHAGCPLCEAAGGVRVAQAARWRVIRAEDPAFPAFYRVVWTEHV
                     AEFSALSAAHRSECMDVVCAVEQALITSLQPTKINLAALGNVVPHLHWHVIARFEADS
                     HFPQPIWGPQQRECLSPMALLRVGLAELDDRVAAAVSPICQRL"
     gene            28849..29262
                     /locus_tag="PKV56_00520"
     CDS             28849..29262
                     /locus_tag="PKV56_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOX66315.1"
                     /translation="MAGLTADTPTPTAVTVHQQSRVLQIGFADGSEFRIPFELMRVYS
                     PSAEVQGHGPGQEVLQTGKRDVELVELEPVGNYAVQPTFSDGHNSGIFSWDYLYFLGS
                     QQADLWKQYEERLAASGTDRDAPMAAAAGASCGSH"
     gene            29282..30013
                     /gene="ubiE"
                     /locus_tag="PKV56_00525"
     CDS             29282..30013
                     /gene="ubiE"
                     /locus_tag="PKV56_00525"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOX66316.1"
                     /translation="MSNTHFGFKSVEESQKASRVRGVFDSVASRYDVMNDLMSMGLHR
                     VWKAYTIAVANARLGDRVLDIAGGTGDLALAFSKKVGPGGTVVHTDINEAMLRQGRDR
                     LTDAGVLLPTAICDAEALPFPPATFDLVSVAFGLRNMTHKDRALTEMNRVLRPGGRLL
                     VLEFSRVAKPLEKAYDWYSFNVLPKIGQWVAGDADSYRYLAESIRMHPDQGALKAMMK
                     SAGFAHVDVHNLSAGVVALHVGIKC"
     gene            30065..31066
                     /locus_tag="PKV56_00530"
     CDS             30065..31066
                     /locus_tag="PKV56_00530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX66317.1"
                     /translation="MISNLDKPDSSRDDRKPGRLVLGALLVSFAALVGCNPKQEEATA
                     SQPAAKVNKEEITVAQINFVLQQQRGLKPEQTEAASRQILQRLIDQELAFQKAQEVGL
                     DRDPRVLQLLEAARREIVARAYVEKAGQAAAKPSADDVKKYYDEHPALFSARRIYTLQ
                     EVVIETKPEQLPALREQLGAAQTISEFIEYLKANSFRFAGNQAVRTAEQLPLNMLDAI
                     AKMNDGQAMLLQTAVGAQVIVLAGSRSEPVDQARATPVIEQFLLNDAKRKAAEADIKA
                     LRAAAQIEYVGTFASPAASAPAAGEPLPSLTAPASAAQDGTSLPLEAESIGKGMGLK"
     gene            31063..31911
                     /gene="epsE"
                     /locus_tag="PKV56_00535"
     CDS             31063..31911
                     /gene="epsE"
                     /locus_tag="PKV56_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HOX66318.1"
                     /translation="MMQLRAVRSVLAIVAFGAALAGSPAFAAQVGGGSAPAASAAAEY
                     RLGAGDLVRVNVYQNPDLSLETRIGEAGNTSFPLLGSVKIGGLVVSAAERLIADGLRN
                     GGFVKQPQVSVLVLQVRGNQVSVLGQVNRPGRFPIEVADMRLSDLLAMAGGISAIGAD
                     TVILVGARDGKPIRRELDLLSIFRSASRDEDIVVQNGDVIYVERAPTAYIYGEVQRAG
                     PMRVERGMTVMQALATGGGLTQRGTEKGLRIHRRAADGRVQIVQPAMDDALRDGDVVY
                     VKESLF"
     gene            complement(31922..33244)
                     /locus_tag="PKV56_00540"
     CDS             complement(31922..33244)
                     /locus_tag="PKV56_00540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOX66319.1"
                     /translation="MKITIIGTGYVGLVTGACLAEMGNHVVCLDLDAAKIKILEDGGI
                     PIHEPGLLDVVKRNRAAGRLQFTTDVVAAVNHGTLQFIGVGTPPDEDGSADLQYVVAA
                     ARNVGRHMTDYKVIVDKSTVPVGTGEKVRAAVAEELAKRGVELEFSVVSNPEFLKEGA
                     AVEDFMRPDRIVVGADDERAILLMRSIYSPFMRNHDRLLVMDIKSAEFTKYAANAMLA
                     TRISFMNELALLAERVGADIELVRKGIGSDPRIGTHFLYPGAGYGGSCFPKDVKALVH
                     TARDNGVTLQVLTAVEAANEKQKHVLVDKIVKRFGEDLSGRQFAIWGLAFKPNTDDMR
                     DAPSRVVIAELAKRGAKVKAYDPVAMTEARRVLEGVRGLDFVDNQTQALEGSDALVII
                     TEWKEFKSPDFDAIKSMLKQPVVIDGRNLYEPALMRALGIEYSGIGRA"
     gene            complement(33278..34738)
                     /locus_tag="PKV56_00545"
     CDS             complement(33278..34738)
                     /locus_tag="PKV56_00545"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOX66320.1"
                     /translation="MSSISVHPVIMAGGSGTRLWPLSRAQHPKQFLVLKGNQSLFQQA
                     AQRLASLATTDIAVAPPLVVGNEEHRFLVLDQLRELKLPPASVLLEPMGRNTAPAMTL
                     AALHATADGADPILVVTAADHAYVDEGGFTRALQSAVRAASEGAIVTLGIQPTGPETG
                     FGYLRCEAAGSQSASPAQVVAEFVEKPDRATAERYLADGRYYWNSGIFVVRASVWLKA
                     LEQFRPDISKATRAAWELRTTDASFIRPDRAAFEAIPSESVDYAVMERCPGSPEFDIR
                     MVPMNAGWSDLGAWDAVWEVSTKDGNGNASHGDAMLRDCRNTLVHATSRLVGVIGLDD
                     VIVVETPDAVLVTRRSSSQDVKQLVNQLQQDGRSEHTLHRQVHRPWGWYDSIDSGSRF
                     QVKRIMVKPGATLSLQMHHHRAEHWIVVRGTAEVTNGDSVILLTENQSTYIPLGQAHR
                     LSNPGKVPLEIVEVQSGSYLGEDDIVRFEDTYGRAK"
     gene            complement(34816..35838)
                     /gene="galE"
                     /locus_tag="PKV56_00550"
     CDS             complement(34816..35838)
                     /gene="galE"
                     /locus_tag="PKV56_00550"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOX66321.1"
                     /translation="MRSTVLLTGATGYIASHTWLALWAAGFDVVGVDDFSNSSPEVLN
                     RLQRLSGRSANFERADVCDTASMASVFQRHTIAAVIHFAAFKAVGESSAQPLAYYRNN
                     LGGLFSVAGSLAQSGAKKLVFSSSATVYGTPERLPIPEDSPLSTTNPYGATKLMGENI
                     LRDIERADPSWRIALLRYFNPVGAHESGLIGEDPRGTPNNLMPYISQVAVGKRPRLQV
                     FGNDYDTPDGTGVRDYIHVTDLAEGHVAALRYLLDGGGRSLTVNLGTGQGCSVLSLVR
                     AFEKASGRPIPYDIVARRPGDVAACYADATLAREYLGWQATRDIDTMCTDTWRWQSLN
                     PNGYTD"
     gene            complement(35835..36989)
                     /locus_tag="PKV56_00555"
     CDS             complement(35835..36989)
                     /locus_tag="PKV56_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66322.1"
                     /translation="MLAMLMWPLAGPAHAEANPFYVGADLSAVHDSNVFRLKKATSDT
                     SYSVGLLAGLDQPIGRQRLFANVRLRETRFQDLSRLDHTSYAINAGLDWAAIDKLSGT
                     LSFSGADGLFNYGGTNTAQSTERNIETRSESLARIRYGSGSHLAVEASLSHRSLRYSN
                     PAYEASALTQDTAGMALYYQPSAGLRLGTGLRLSRGEYKGSGLDFDRRDIDLTATWLA
                     TGLSTLDGRFSVGRRESRGATSALDFSGATGQLIWTYRPTGKLLFKTAFSRESGAESS
                     FLDVAEAQNGSVGDASRITNSLAVSAAYALSAKIHVDAGVRANHRSLVAGSIDGSDTL
                     RAASLGVTYEPLRHLKFSCQVGRETRSTAGSLSFGYSASSATCTAEIKLQ"
     gene            37281..37865
                     /locus_tag="PKV56_00560"
     CDS             37281..37865
                     /locus_tag="PKV56_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66323.1"
                     /translation="MLSALNAGIAAAVTARATLLINHVLSSEPVACGRLRAHAGRRLR
                     IRLLDWPGLLPALPALCFAITAAGLLEWQPEVDGAGPDSQVDLDITVEAGNPAALLFG
                     GLVGQRPAVTISGDSSLAADVSWVIDNLRWDLRDDVARLIGEIPARELARQASAMAAG
                     LRGLVQDVVQRATSARGGVGGAAQSPSPAQRPAR"
     gene            37862..39415
                     /gene="ubiB"
                     /locus_tag="PKV56_00565"
     CDS             37862..39415
                     /gene="ubiB"
                     /locus_tag="PKV56_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HOX66324.1"
                     /translation="MRHLARLLFIVFTVYRYGLDELALSNFRQRWVRSLVRLGTLGRR
                     LDAPRGERLRLALERLGPIFVKFGQVLSTRRDLIPPDIADELAQLQDNVPPFPAALAR
                     QMIEKAYGRSIEDVFATFEAEPVASASIAQVHFATLKDGREVAVKVLRPNMLPVIDDD
                     LSLMRTMAVWVERLSADGKRLRPREVVAEFDNYLHDELDLVREAANAAQLRRNMDGLN
                     LVMLPEMHWELCTQTVIVMERMHGVPISQTQRLRDAGVDIPKLARDGVTIFFTQVFRD
                     GFFHADMHPGNIQVSLAPATFGQYIALDFGIVGTLTEFDKEYLAQNFIAFFRRDYKRV
                     AELHVESGWVPPNTRVDELEGAIRAVCEPHFDRPLKDISLGQVLLRLFQTSRRFNVEI
                     QPQLVLLQKTLLNVEGLGRQLDPELDLWNTAKPFLERWMNDQIGWRGLISRLKNEAPR
                     FVQLLPELPRLLHQSLQPKPVAEQRTLEALLAEQRRTNRLLQAIVYAGLGFLIGVLVV
                     QWFMHVGYR"
     gene            39466..39861
                     /locus_tag="PKV56_00570"
     CDS             39466..39861
                     /locus_tag="PKV56_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOX66325.1"
                     /translation="MPIYAYRCEACGHAKDVLQKISDPLLTLCPACGAESFKKQVTAA
                     GFQLKGSGWYVTDFRNGSGGGAPSDKNAGDSNPAGEGAGKGAASGAKEGSASPDAAGS
                     PSSPAPAAAPAAPSPAASGSSSAPSSSST"
     gene            39906..40559
                     /locus_tag="PKV56_00575"
     CDS             39906..40559
                     /locus_tag="PKV56_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOX66326.1"
                     /translation="MKKYLLTGLLVWLPLTITIWVLTSVLGVLDGLFAWLISASQAVL
                     PAAAHSTLEMLKQIPGLGVIVMLVGLLLTGIFATNIIGQWWLGQGSRLLNQIPIVKSI
                     YSSVKQVSDTLFSSSGNAFREAVLVQYPRQGAWTIAFVTGRPGGEAGLHLPGDYLSLY
                     VPTTPNPTSGFFLMVPRADVVPLKMSVDEALKYVISMGVVAPPTAPASSPPERNQPG"
     gene            40693..42495
                     /gene="aspS"
                     /locus_tag="PKV56_00580"
     CDS             40693..42495
                     /gene="aspS"
                     /locus_tag="PKV56_00580"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOX66327.1"
                     /translation="MRTTYCGLVSEALMGQTVTLMGWAHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRAEMFRTAEGVRNEFCLKIVGKVRARPTGTENANLTSGKIEVLCLELEVL
                     NPSVTPPFQIDDENLSETTRLTHRVLDLRRPAMQRNMMLRYRVAMEVRKFLDVNGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGQFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLNEAEIRAITEAMIRTVFRKTMGVELPVYAEMTYAEA
                     MHKYGSDKPDLRVKLEFTELTEVMKTVDFKVFSGPATTPGGRVAALRIPGGAEMSRGE
                     IDAYTEFVKIYGAKGLAWIKVNDAGKGRDGLQSPVVKNLHDAAIAKIIARTGCCNGDL
                     IFFGADKAKVVNDALGALRVKIGHSDFGREHGLFNDVWAPLWVVDFPMFEYDDDAKRW
                     NAVHHPFTAPKDGHDELMKTDPGACIAKAYDMVLNGWEIGGGSVRIHRADVQAKVFSA
                     LNITPEEQQVKFGFLLDALQYGAPPHGGLAFGLDRLVTLMTKAESIRDVIAFPKTQRA
                     QCLLTGAPSAVDEKQMRELHIRLRNAQAAPQNAS"
     gene            42503..43306
                     /gene="minC"
                     /locus_tag="PKV56_00585"
     CDS             42503..43306
                     /gene="minC"
                     /locus_tag="PKV56_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOX66328.1"
                     /translation="MARSKERAPAVFELRSASMTLVVVALKTTDMAAIEAEMTARFSD
                     TPDMFNHDPVVIDIAQAEGDPMALDYGRLVSLLRRFNMVAAAVRGGSPEQMSAAAEAG
                     LGEAPEAVVPAARPVVQAPAAVEAPPVAVEAVTASPIEVSPIPAAPAVPQLAPAHVAT
                     MIVDKPLRSGQQIYARGGDLVVLAVVNFGAEVIADGSIHVYAPLRGKAIAGARGNTDA
                     RIFSTCMEPELISIAGTYRTTDNPLPDNVIGKPAQIRLDGERMVFEPLD"
     gene            43381..44196
                     /gene="minD"
                     /locus_tag="PKV56_00590"
     CDS             43381..44196
                     /gene="minD"
                     /locus_tag="PKV56_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOX66329.1"
                     /translation="MSRIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIHGEAKLNQALIKDKQCDNLYVLAASQTRDKDALTQDGV
                     ERVLEELAGMDFEYIVCDSPAGIETGALMAMHFADEALVVTNPEVSSVRDSDRILGML
                     HSKTRRAIEKKEPIKEHLLITRYNPNRVLGGQMLSLKDIHDILRVRMIGVIPESESVL
                     DASNQGVPAIHIKGSDVSEAYKDVVTRFLGEETPLRFVDAAKPSFFKRMFGGK"
     gene            44200..44460
                     /gene="minE"
                     /locus_tag="PKV56_00595"
     CDS             44200..44460
                     /gene="minE"
                     /locus_tag="PKV56_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOX66330.1"
                     /translation="MGFLSFFLGEKTKTASVAKERLQIILAHERNGRTRDPAYLPDLQ
                     RELLAVISKYVSVGPGDIKVEVERQGDLEVLQVKIELPEAAR"
     gene            44474..44947
                     /gene="nudB"
                     /locus_tag="PKV56_00600"
     CDS             44474..44947
                     /gene="nudB"
                     /locus_tag="PKV56_00600"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HOX66331.1"
                     /translation="MNDSRPPKIPESVLVVIHSADLQVLLIERADKPGFWQSVTGSKD
                     AVDEPFAETARREVAEETGIVIGSADVPLAHLRDWGMRNVYEIYPVWRHRYAPGVTHN
                     TEHVFGLLVPRDIPVTLAPREHVRSEWLPWREAADRCFSPSNAEAVLQLPRFAAA"
     gene            44980..45738
                     /locus_tag="PKV56_00605"
     CDS             44980..45738
                     /locus_tag="PKV56_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX66332.1"
                     /translation="MHPSDVLRVATYNIHKGVRGVGPRRRLEIHNLALGIEALDADLV
                     FLQEVRSSNRAEALQFPDTHIGWPRMPQADYVAPEGYAVAYRTNAITRHGEHGNALLS
                     RWPIGDIRHHDVSDHRFEQRGLLHVPVQWNGTLVHAIVAHLGLMHASRVRQVERIAAF
                     ISLHVPPGEHLVLAGDFNDWGEKLDAPIRRMGLTPAHAAGAPPRTFPSRVPFFSLDRV
                     YTRGLRCIATHVPRSAAWARMSDHLPLVAELQLV"
     gene            45756..47066
                     /gene="clsB"
                     /locus_tag="PKV56_00610"
     CDS             45756..47066
                     /gene="clsB"
                     /locus_tag="PKV56_00610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HOX66333.1"
                     /translation="MATDASSARAAADRSALEQLWGAVPRPVFSGGNAVRLLRGGEEL
                     FPALVEAIDEARHEVCLATYIFHDDLAADSVTQALIRAAQRGVQVKVVLDGFGMLGRL
                     SELQRRLTEGTVQVAVYRPLDRWWNWLQPGQLRRLHLKLCVADAQIAFVGGINLMDDR
                     FDLNHGWTEEPRLDFAVRVTGPVVGPIHQATIAAWTRAHLGSAFKEEALAIVRGAEPM
                     ARVRRLIRRLRLSRGQGMQVTPGVPSPMQAAFVLRDNVRQRRTIERSYIDAIAKAGKR
                     VDLVSPYFYPGRSFRRALKHAAQRGVAVRLLLQGKVDYRIASLAARALYDELLGQGVR
                     IFEYTPAYLHAKIALVDDDWATVGSSNIDPLSLLLNLEANVIVRDGDFNAQLSAQFDA
                     AVAVSDEIDPKLKYRTGVLGALRRAVVAWVAYIYLRVAGATGRY"
     gene            47169..48287
                     /locus_tag="PKV56_00615"
     CDS             47169..48287
                     /locus_tag="PKV56_00615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HOX66334.1"
                     /translation="MTSADLVVERPAGLYCPPGDFYIDPWRPVDRAVITHAHADHARS
                     GHGHYLASARSAGVLRSRLGAITLQPLAYGEVVEHRGVRISLHPAGHVLGSSQVRLEH
                     GGRVWVASGDYFATAAAGAPGDEDNLTCEPFEPVRCDCFITESTFGLPVYRWRPQREV
                     YADINAWWSANAEAGRASLLMGYSFGKAQRLLAGLDSRIGPIVVHAAVEPLNRAYRAE
                     GVQLPVTQLLGEVTDRAGLRRAIAIAPPSAQDSAWARAFTVGGVEASDAFASGWMQLR
                     GARRRRAVDRGFVMSDHADWPGLQRAIAATGADRVIVTHGYEAVMVRWLEQQGLQAGS
                     FRTEFGREVGAEEASGPLPEGTASGVEGSVAKPDGAVP"
     gene            48284..50050
                     /locus_tag="PKV56_00620"
     CDS             48284..50050
                     /locus_tag="PKV56_00620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOX66335.1"
                     /translation="MRRFSALFAELDATTATSAKVDALKRYFASAEPADAAWAVYFLS
                     GGKPRRVVPTAVLRSLACARAGIDDWLFDECYQAVGDLAETIAHVLAPPSRHSDLGLA
                     VWVEHRLLPLRGEPPETQAARIAGYWDELDAAERFLLIKLIGGGFRVGVSKLLVQRAL
                     AESVGIDARVVAQRMMGYTDQAARPDAAGYTRLVSLDAEPGRDAGQPYPFFLAHPVDD
                     AVLADFDAALGPAGDWLVEWKYDGIRAQVVRRAGEVWIWSRGEELVTDRFPEVVTAAQ
                     AWSDGTVIDGEILAWLPDSDRPAPFNLLQQRIGRKNLTKKILAGAPTTFMAYDLLEHA
                     GVDLREAPQHERRAALEALGAAIPALRLSPLLRGSWPELAALREQSRARGVEGFMLKH
                     REARYGSGRRKQVAALPGSPSPAWWKWKVDPLTIDAVLIYAQAGHGRRASVYTDYTFA
                     VWSRAPRDAAEAQAVVDAIEARQPAQPDGLQLIAFAKAYGGLTDEEFKAVDRVIRQTT
                     LEKFGPVRSVRPTMVFELGFEGLNISTRHKSGIAVRFPRMLRIRGDKPLREADTLDTL
                     RALMNPGDPADEHDEHDPGDGL"
     gene            50013..51452
                     /gene="gorA"
                     /locus_tag="PKV56_00625"
     CDS             50013..51452
                     /gene="gorA"
                     /locus_tag="PKV56_00625"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HOX66336.1"
                     /translation="MSTTNTTPATDFDCDLLVIGAGSGGVRAARFAAQRGARVIIVEG
                     GPLGGTCVNVGCIPKKLYSYAAHHGEAFEQAHGFGWEATTPRFDWAVLKARRAAEITR
                     LNGIYDGLLKSAGATLLRGWASVADAHTVAVRPVDGAAGSSVKHCTARHILLATGGRP
                     QKVDGPGADWALTSDDMFDLDPFPRRLVVVGGGYIACEFASIFNGLGAQVTLVHRGPR
                     LLRGFDHEVADFVTAEIRKKGITVHLQSTIADVRRAGAVQTLRLKQRAAAADAGAPAD
                     IELEADAVLHATGRIARTDGLGLDALGIMLNPNGTVPVDPHFQTVVPSVHALGDLVGH
                     MALTPIALGEAMLLVDHLFGDGVRRPIDYSLVPTAVFTHPNIGTVGLGEEAARERGPV
                     RIYRSEFKPLQHTLSGSTERTLMKIVVDDVTDRVLGMHMVGADAGEVIQGFASAMQAG
                     LTKAQLDRTIGIHPTGAEEFVTMREVARR"
     gene            complement(51560..53095)
                     /locus_tag="PKV56_00630"
     CDS             complement(51560..53095)
                     /locus_tag="PKV56_00630"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOX66337.1"
                     /translation="MTTTAFPASGPSDSLYDPTIGAKLKSLLDRYGIGLLGESGRLRG
                     LLHDDCPQAKREVSVLMQALEERVPQDLMRVHSGEPIQSLTPRLAKRLSDEKGMAPNA
                     SRWAVDTWAEGLGLEALISNAAFADGGTGSAQALFRSAANQTFGGPADPVSAPVPVPP
                     NRKLQAAVAGLALCGVIAFSWFGFLRPVFEITAVEMQGPMVGDGKARPVTLAIHARRE
                     TVKDVEVRFVSGEGNWNNQVASYPATEATSGEAKVGAGTLAYKTAQPMNATFEYVLVS
                     TDGKRSAPFQRTFNILPPIVISSVTAPRPLGVGRAFNVDIKYQKGAADIVQIERRVVD
                     SSVPWPDVSATAAVQPGNATGAVQFPFDAAKQPMRSTLEFVLVDAQGVRSEPARLTLD
                     IGTAANGGGGPAVVMAVQQVTRTGSSSGVGAVVGGVLGGVLGNQFGSGSGRAVATIAG
                     VAGGAYAGNTIEKNNNSTSGWETTVKFDDGSRRTIRHSTAPAWTVGQRVTVDNGAISL
                     ASN"
     gene            complement(53226..54569)
                     /locus_tag="PKV56_00635"
     CDS             complement(53226..54569)
                     /locus_tag="PKV56_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66338.1"
                     /translation="MPALRPGQLLRCVDSQLACRVEVLIGEGGQGEVYRVTLDGRPYA
                     LKWYHPLALRVDRGLRQRLQVALERGAPTTEFLWPFELVTLPDGSGLGYLMRLRAESH
                     LKIQSVLAGEVRPTFRVLATVCCRLTEALLALHTKGLVYQDLNAGNLFFDRSTGQIEI
                     CDNDNVDIDGAPSVMGGVWEFQAPEVVLRQCGPSRATDLHSLAVMLFRILHIGHPLIG
                     RCELEHHNLTDQAVLRRLYGSGARFVFDPSDASNRPLPDRHGPVLGHWAIYPQALRDL
                     FTRAFTDGLFDPLHGRVQESEWRKALRQLHDSVLTCRGCGAENFHDPQRLAAKQHQFA
                     CWDCGAALSTSPPRIGLRRLQARSQEAPLYVVVLDPGARLLSHHVDDQNSGHPSSAAQ
                     IVVDPALALVNLSRQTWSVWSGPSTQVEKVEPGERLSLSQGQRIDFGRMLGEVKL"
     gene            54733..56340
                     /locus_tag="PKV56_00640"
     CDS             54733..56340
                     /locus_tag="PKV56_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOX66339.1"
                     /translation="MEQKRPGGDMKSPASSEPTRSQLLPLGGAWQELLTKPYFIPGLV
                     VSLAIALLFSFRGDKSATINVSYGGIPVGVPAYTIVLALFIVFGGAYGVYRMAGKSKA
                     WWLMPVVAIFTGLLAFSPLMGMLQDVYGVVLGRSSKADGWVLKFVQSLFNAGLPEETL
                     KAIPVAIGVYIGISLLGRFKAGHPARQLAVIEPLDGILIGVASGFGFAFAETVFQYVP
                     GVILNSQETVRGLLYALKTIGYPVRLPQDTSQLQDIAALFSLLANKIGFERATFELAQ
                     ILQNRQGAGLELMIPRLLSGLFGHAAYAGIFGYFIGLAALKPGARVKTVLIGVAVAAT
                     LHALWNSSAGSVMMFFVLSMAAFVCLAVTIIKARAISPERDQLAASQLIDRFAPGGMS
                     TIRPVERAMPAAAPDANAVAERTTAHPAMVPASPAAQIGSLTWDDDSNLRVLEVGTAR
                     IPATAGSHLYERQVPGARSGQGDAVVAEVTAHPNDPSVLGLKNLSDQSWSLVTVEGEQ
                     RELAPGRSIRLVAGLQVRIGDLSVRVR"
     gene            56350..57057
                     /locus_tag="PKV56_00645"
     CDS             56350..57057
                     /locus_tag="PKV56_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOX66340.1"
                     /translation="MNDTAELNVPRPGGAMARRELHFIWLLDTSGSMNSDGKIQALNV
                     AIREAIPHLVSAARDNPNVDVRVRAMTFSDGARWHIETPTPVHDLKWIDVAAGGHTDT
                     GTALKLLAQALKVPPMPERAVSPVLVLVTDGHHTDDFEAGLAALLAERWGREAVRMAI
                     AIGRDVHLASLQQFIGNDEIRPVEASHPEALVQQIRWVSRSGIDSVSQVLDGGRRLTV
                     GDLANNSAGTVDDAVEW"
     gene            57057..58358
                     /locus_tag="PKV56_00650"
     CDS             57057..58358
                     /locus_tag="PKV56_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOX66341.1"
                     /translation="MSAEAVTPWQVVGRSVRGATHLRDGLPNQDAVAHRTIVEGGQPA
                     LAIAAVADGHGGRRHFRSADGARMAVEVALQVLCSMATHFEQLSPADRSELAAAQVPM
                     AIVSAWTDAVRRHLATCPISDEEWLALDAAEGSEGSAAVRADPLLAYGATLLAALTTP
                     NSVALWQLGDGDVLAVSPDGSVNRPVATDERLLGNLTTSICRPGAEADFRSVVLSSHS
                     ALPALLMLSTDGYANSFRTDADFLKVGADLLELISKHGLSAIDARLGGILEDASANGS
                     GDDITAALLYRSGSEPSVLSTSGGGWPPAELAVASAAPSDGGQALAAAQRRIARLRSV
                     IVVLLVVLLAGALWWSRDRSRPKSADLPALGAPIGWDAPASGPKPSVEAAEPAPVVSP
                     QGAAKPPLAAKPKPAASAASRPKPATAPAATSKPTPELPPY"
     gene            complement(58409..59086)
                     /locus_tag="PKV56_00655"
     CDS             complement(58409..59086)
                     /locus_tag="PKV56_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOX66342.1"
                     /translation="MTPDTEPSMKEAARLIRHVVSSGAHLSYEQLEAHVDGRAEPALR
                     ERVETHTAWCARCATELQDLAAHAPRLRQRVESRTASSGWFAWLKAGPRLLPQIALAS
                     VVAAVAITVVMQDPRLPADDHTTHAQPVPDGSDVMTRLAEVSAEAVEANRRGDHAKLV
                     RLLKDAAERGNVTAALALGTLYAQGLGVAVDIVAAERYWQQAAAAGSVEAQRHLDELR
                     APHSMKR"
     gene            complement(59166..60272)
                     /locus_tag="PKV56_00660"
     CDS             complement(59166..60272)
                     /locus_tag="PKV56_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66343.1"
                     /translation="MGNAEETSSLLLQVGETVRAILRRKSGLSLRDDDPRPENQEAVE
                     LVHDVILRLWERQSTTPGAAWDDLGSYAARVTYNAWSDHLRDKYPKRASLKNRLRYFT
                     SHQARYASWNASDGELLIGLHGWQLSSRVPVGDARVAALRDGRERLAAGSVPRKDMER
                     FAAEDWDHLLAALFERLQAPIALDDLVSVTVALLKVQEDRTDSLDAMLDDETTPDLPD
                     PTALSPQQAAEVRSMLRHLWGAVLVLKPDYRTAYLLNLPGVGKSRGDIEVFVMHDIAS
                     ICEISAAVGLDAGQYRIAFAQLDLDSAARAAVERCSADVHRFEILWDHLPLMDALIGA
                     LLRLDAQQVINRRMLALRELARLMAHTSNRLKAP"
     gene            complement(60376..60918)
                     /locus_tag="PKV56_00665"
     CDS             complement(60376..60918)
                     /locus_tag="PKV56_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66344.1"
                     /translation="MNIFTRTCTTALAAVILAVGLPAADAQAGGRHGGGHGGHGRYYG
                     PGWGGVGLGFGLGLGLGWLGASRYSYSYGPPVYAGYAEPGYVLIDPPPVVYREVPPVV
                     YREAPRTVYREVPAREPVVVARPDPIIYPRNGQSAQQTEADRQECNRWATTQPSAMSD
                     ASVFNRATEACMDGRGYTLR"
     gene            61037..62359
                     /gene="guaD"
                     /locus_tag="PKV56_00670"
     CDS             61037..62359
                     /gene="guaD"
                     /locus_tag="PKV56_00670"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HOX66345.1"
                     /translation="MTSRIALRGDLLDFTADPGFAPTSEPPGLRWRPDHWLMIEDGRI
                     INALPAGSVDTMADGGPDASWQRVDHRGRLILPGFIDTHVHSPQLDVIASHGGALLDW
                     LERYTFPAEQRHADPAVAEAGARRFVDALLAHGTTAAVVFPTVHKVSAEALFDAAQRR
                     GMRLVCGKVLMDCRAPEGLRDDVAQAERDCTELIERWHGHGRLAYAVTVRFAGTSTRE
                     QLTMAGRLCVAHEGVYMQTHVAENTDEVRWIAGLFPEARSYLDVYERHGLLHERSVLA
                     HGIWLDDTDRALLRERGAQIAFCPSSNLFLGSGLFDWQAAADAGVQVSMASDVGAGTS
                     LSMLRTLADGYKVQSLRGLRPSAWKLLHAATLGAAQALDLDAEIGSLQTGCVADLTVW
                     DWAVGPIATHRDGLARDLHERAFAWMTLGDERNLVATYVAGTSVHQRA"
     gene            62379..63446
                     /locus_tag="PKV56_00675"
     CDS             62379..63446
                     /locus_tag="PKV56_00675"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOX66346.1"
                     /translation="MSPNLPAGIDAIPSGRLPALLREMPKAELHIHIEGSLEPELIFA
                     LAQRNGVPLAYASVDALRSAYAFSDLQSFLDIYYAGASVLLHEADFYEMAMAYFRRAA
                     ADHVVHAELFFDPQTHTERGVPIEVVINGLVRACKAAEAEFGITSLLILCFLRHLSEE
                     AAFETLEQALPSIREHGDRLVGIGLDSSERGHPPEKFSRVYARCRELGLKLVAHAGEE
                     GPPAYIWSALDTLKVARIDHGVRCVEDPALVARLAAERVPLTVCPLSNVKLRVFDTLA
                     QHNLARLLDLGLCATVNSDDPAYFGGYMNQNLVETFAALPELTAAHAYTLARNSFDAS
                     FASASQKARWVQALDACFAQG"
     gene            complement(63463..63807)
                     /locus_tag="PKV56_00680"
     CDS             complement(63463..63807)
                     /locus_tag="PKV56_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66347.1"
                     /translation="MATRSGLFALPEETRTSMRRWIFVGMAVMMLAGVAFAFWPFMAT
                     AQRMQGFCEALAAGTPLADVQAQAATRGYTLSTLADGRVLVQDPRMSGRRTCALQFGD
                     QGLQSSKVTDQP"
     gene            complement(63848..65053)
                     /locus_tag="PKV56_00685"
     CDS             complement(63848..65053)
                     /locus_tag="PKV56_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HOX66348.1"
                     /translation="MDPYLTDWLNLLLRWAHVVVAIAWIGSSFYFVFLDNSLTPPADE
                     ASKSRGVSGELWAVHGGGFYHPQKYAVAPPALPDKLHWFYWESYSTWLTGFGLFTLLY
                     LLQAGTYLIDPKVHEWSAAAAVASALAVLALFWFAYDMLCRTLGTGSNGDRIAGFGVA
                     LLVGITSVAVCALFAGRAAFLIVGAAMATAMSANVLIWIIPGQRRVIAQMQAGTAVDP
                     IHGQRAKQRSVHNTYFGLPVLVAMLGNHYPFLYSAPHSAAVLMLLMSAGALIRHSFVA
                     RRRALALGRRVPWEHAILGVLLLIGTAVWLKPPSPPASSPAGSPVTLAQVQTIIDQRC
                     VMCHNATLANKGVALHTDRQVEQHALAIYQQAVVLKQMPMNNATQLTDQERDTLRRWF
                     EAGTATRRE"
     gene            complement(65080..65433)
                     /gene="uraH"
                     /locus_tag="PKV56_00690"
     CDS             complement(65080..65433)
                     /gene="uraH"
                     /locus_tag="PKV56_00690"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HOX66349.1"
                     /translation="MATLSTHALDTMHGCPAAGMAVALYSVGSESVSLLHRSVLNDDG
                     RAPDLAQAASALAAGRYRLVFQVAAYFRLRGAVLPDPPFLDEVGIDFGIGEAGGHYHV
                     PLLASPWAYSTYRGS"
     gene            65552..66112
                     /gene="uraD"
                     /locus_tag="PKV56_00695"
     CDS             65552..66112
                     /gene="uraD"
                     /locus_tag="PKV56_00695"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HOX66350.1"
                     /translation="MNAHHDRALNLDRINTAPLADVLALLDGLYEHSSWVVEAALAGR
                     PFATLADLKHAMAEVVRHAGHERQLVLIRAHPELAGSALTAGTLTRDSSHEQSLSGLA
                     HGSPDEVHALHELNNEYTARFGWPFVLAVRGPTGRGLTRAEIIAALRHRLVATPEVEM
                     DECLRQIDRIAEIRLHERMGLGLEAG"
     gene            66109..67677
                     /gene="xdhA"
                     /locus_tag="PKV56_00700"
     CDS             66109..67677
                     /gene="xdhA"
                     /locus_tag="PKV56_00700"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HOX66351.1"
                     /translation="MNGQPIRFFHRGAITEVSGVHPTLSVLDWLRDHARCTGTKEGCN
                     EGDCGACTVVIGELPERTDRPAEHVRGLSLRSVNACLQFLPTLNGKALFTVEDLKTVA
                     GGALHPVQQAMVECHGSQCGFCTPGIVMSLFADYEQHRTAGRRPDRQQVADALSGNLC
                     RCTGYRPILDAGERLFDLPSVQLDTAPVIAALRSLQQTGTFSYGAANRHGPAEGGGSR
                     IDHFHAPTSLADLAALRLANPAARLLAGSTDIGLWVTKQFRDLGDIVYLGNVTELKQV
                     ETRDGELHIGAGVSLETAWRALAERWPGVAEVGLRFAGPPVRHAGTLGGNVANGSAIG
                     DAAPVLMALDAQIELRRGDTVRRLPLQFFYVDYMRNLMQRGEFVQGLAVPLATSRRSV
                     RAYKISKRHDCDISALCAAFAIDIEGGTVRSVRLAYGGMAAVVKRAAAAEAALVGGAW
                     SAESVKAAQRALVQDFQPLSDLRASADYRLQVAGNLLQRFWLETRAVDPLPAQATRVW
                     PIRSLDSASVDTPP"
     gene            67674..>69720
                     /gene="xdhB"
                     /locus_tag="PKV56_00705"
     CDS             67674..>69720
                     /gene="xdhB"
                     /locus_tag="PKV56_00705"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HOX66352.1"
                     /translation="MNDSLAAAGPGSPAEAVEPAGLVSAGCAGVGVARPHESARLHVA
                     GEAPYTDDLPELVGTVHIALGLSPVASGRLIGLSLDRVRAMPGVVAVFTGADFPGPND
                     CGAIVHDEPILATISFDGPNSCVGELRYLGQPVFAVVADTRDAARRAAAQAPHVMTIV
                     PLPAVLTMQEAHARSDYVLPPLHLNRSISGRGAHAAIEGAPHRLSGEFEIGGQEHFYL
                     EGQISCAQPTEDDGLLVRCSTQHPSEMQHVVATALKLPAHRVRVECRRMGGGFGGKES
                     QSAPFACIAALVARRLDRPAKLRLDRDDDFMVTGRRHGFHIDYDVGHDGEGRVLGAEV
                     TLLANAGHSADLSAPVMSRALCHVDNAYWLPEVALHGFCCRTNTQSNTAFRGFGGPQG
                     AIAIEFILDGIARRLGLDPLDVRRANFYGVDERNTAPYGQLIADNILQPLVDRLDRTS
                     DYRTRRAAIALFNAGSPVLKRGMALTPVKFGIAFNLSHLNQAGALVHVYTDGSVLVNH
                     GGTEMGQGLNTKVAQVVAHELGIAFERVRVTATDTDKVVNTSATAASTGTDLNGKAAQ
                     NAARRIRDRLAAFASGLHGGEAAGVIFANDAVRVGDRTLSFTDLVAQAYVGRVQLWSD
                     GYYATPGLHWDRETMQGHPFQYHAWGAAVSEVVIDTLTGEWKLLRADLLHDVGTSLNP
                     "
BASE COUNT        11803 a        22638 c        23036 g        12243 t
ORIGIN      
        1 cctggccgtt cttcatcgac ggcagcgtca cgccattctg gacgtgcccg tgatcgttgg
       61 tcgcgatcac ccgtccgttg tctgcgagca gcacgagcgc aatctcctgc ggcacctgtc
      121 cgtctcctcc gttgaagatc cggctcatct cgtcctcgaa tcggaagcgc agcaccagca
      181 cgcctgccgg gcggccccct gagccattga tgcggtgcgc atacagaagt gccggggact
      241 ggtccctgcc cagatcgctc gggccgaaac gctccacgta ggcatgggcc gcgagcgcat
      301 cggcaacgat cgcatcggag ctgatcgcga ggggggcctc gcggtcgaga cgggcgagca
      361 cgactcctgc gggagacagc aggatcacgt cgtcgtagac ggtgtacttg gcctggtact
      421 cgaaaagtcg atgcaccatc gagtcgcggg aggcttgctg gtcgtctgcg gtgccctggc
      481 agaacgcgcg gaccacgtcg tcggtcgcca ggaagcccac gtcagcggtg cgctcgtaga
      541 gattgcgcac gagggtgtcg atggcacatt gagccgcagc cgtcagttcc tcgccgagcg
      601 ccgcgcgctg ttcggtcacc atgcgtccga tgagtcgccc ctgcaggtcg ttgaagcgct
      661 cgcgcgtgct gatcaaggtc ggcaggatcg gggcgacttc ttccgggcag ctgatcgccg
      721 cggcagactc gatcatctgc cagagcatcc ccaggtctct caggtcgcgt tctccagcca
      781 gaacacgatg catgtgattg acgaggtcat caggctcgga catcagggtt actcctcgtg
      841 tgaagcccct agtctaggta tgctgcgctg cacacaaggt gtcgagtttc ctggggattg
      901 ccccctaatt cctgaatccc gatgcgttgc gggcggtggg tttcaagaag cccgcacggt
      961 gtgacaagcc gctccagcca ccgccagatc gaggcaggtt cgccccaccc tacaattttt
     1021 gatccccagc gccgcatgaa agccgaatcg atgaaccttc ccaagccagt ttctgcctct
     1081 gccactgcat caccggcggc tcgccccgcc gaaatcgctc cgcgcgacaa ggccgagatc
     1141 ctggcgcagg cactgcccta catccgcaag ttccacggca agaccatcgt catcaagtac
     1201 ggcggcaacg cgatgaccga tcccgaactg cagcaggact tcgccgagga cgtggtgctg
     1261 ctcaagttgg tcggcatgaa tccggtggtg gtgcacggcg gtggaccgca gatcgaggat
     1321 gcgctggcca agcttggcaa gaagggcact ttcatccagg gcatgcgcgt gaccgatgag
     1381 gagacgatgg aggtcgtcga gtgggtgctg gcagggcagg tccagcagga catcgtcggg
     1441 ctgatcaacg tggccggtgg caaggcggtc ggcctgaccg gtcgggacgg cgggctgatc
     1501 cgggcccgca agctgcgcat gcaggatctg aaggatccga ccgtcgaaca cgatgtcggc
     1561 tcggtcgggg aaatcgagtc gatcgatccc agtgtggtga aggcactgca ggacgaccag
     1621 ttcatcccgg tgatctcgcc gctcggcttc ggcgccaaca acgagaacta caacatcaac
     1681 gccgacgtgg tcgctggcaa gctggccgaa gtgttgaagg ccgagaaact gatgatgctg
     1741 accaacatcc ccggcgtgct cgacaagaac ggcaagctgc tgaccaacct gactgcgcgc
     1801 gagatcgacg acctgttcgc cgacggcacg atcagtggcg gcatgctgcc gaagatcgcc
     1861 agcgcccttg acgcggccaa gaacggcgtg aacaccgtgc acatcatcga cggccgggtg
     1921 ccgcacgcga tgctgctgga ggtgctcacc gatcaggcct acggcacgat gatccgatcg
     1981 cattgatttg agcccccgag tggcagcgct cctgtcccgc gggcctggac gggccgcttc
     2041 gtggccctcg ggtgtgtcca caggccgcgc caggcgagtc tggctgttcg acctcgacaa
     2101 cacgttgcac gatgcatcgc actcgtcatt cggcccgacc aacgaggcca tgacgcagta
     2161 catcgtcgat cacctcgact tgccccaccc cgaggcctgg gcgctgcgtc agcactactg
     2221 gctgcactac ggcgcgacga tgctgggcct ggtgcgacac cacggcgtca aggcgtctca
     2281 tttcctcgaa gccacgcatc ggctgcccgg tctcgagtcg cgtctgcgga ccagtgcgca
     2341 cgaccgggcc gcgctgcgcc gactgccggg gcgaaagttc atcctcacca acgcgcctcg
     2401 cgcctatgcg ctgcgggtgc tcgatgcgct gcgcctctcg ggctgcttcg acggcttgat
     2461 cagcatcgag gacatgacga tgttcggaca ccaccggccc aagcccgacg cccggatgtt
     2521 tcgcagcatg gctgctcgac ttggcgtgcc gccgcatcgc tgcaccctgg tggaggacac
     2581 gctggatcac cagcgtgcgg cgtaccgtgt cggcatgcgc accgtttgga tgcagcgcta
     2641 catccctcgc gttcgccgcc tgtccgcccc ccctgaatcg gcgtcggaag tcagtgttca
     2701 cccttgtcac aagcccctgt acgtgtgtgc cagaatcaaa tcgatccagg gcttgctttc
     2761 gatgtagccc accttcccgt ggtgccagcg caatgacgac tcgccgtgac gacaccctgg
     2821 acagcgatgg cgaggagacg cctccggcgg ttgatgccgc ttccgaatcc cctgcagcgg
     2881 gcacctcgcg caagaggccg aaacccggcg agcgcagagt tcagattctc cagacccttg
     2941 ccggcatgct tgagcagccg ggctccgagc gggtgaccac cgccgcgctc gcagcgcgtc
     3001 tgggcgtcag cgaagcggcg ctctataggc atttcgcgag caaggcccag atgttcgaag
     3061 ggctgatcga gttcatcgag accagttttt tctcgcttgc caaccagatc agcgaacgtg
     3121 agccgtctgg tgtgcagcag gcgcgcaagg tggtgaccat gctgctgcag ttcggcgagc
     3181 gcaatcctgg gatggcacgc gtgatggtgg gcgatgcgct ggtgttcgag cacgaccggc
     3241 tggtgatgcg gatgagccag ttcttcgacc gcgtcgaatc gcagctgaag caaagcctgc
     3301 gagtcgcggg cgatgcatcc ggatcggtga tggcaaccgt cgacgcgaat gccgtggcct
     3361 cggtgctgac gtccttctcc gtgggccgcc tgcagcgtta tgcccgctcg gacttcaagc
     3421 ggccgccgac cgagcacctg gatgcggcgc tgaacctgat cctcgcctga gtcctgagcg
     3481 tccagctgtt tcaccgctgg gcgacaggca accaggcagc cagctgttca agtcaccccg
     3541 gcgagggtgg ccgatgtcgc ttcccagggc ccggttccga tgtgaaggcc atcgctacac
     3601 tgcgtgcctc tcgccccacg cgcctgttcg cacgaccccg tatgtcgacc ctccctgatc
     3661 ccctgctgca cctgcttcac cgcgccgaag cactgatttc ccggatcgag gctgttttgc
     3721 ctcatccgtt gacggcaccc gactgggccg cgtcgattgc ctttcgctac cgccggcgca
     3781 gtgctggcgg cagcgcgctc gagccggtgc gccatgtcgc tccgatccgc cttgcggatc
     3841 tggtcgaagt cgaaccgcag aaggaacgtc tggttcgaaa caccgagcag ttcgtggaag
     3901 ggcgcacggc caacaacgtg ctgctgacag gggctcgtgg aaccggaaag agctcgctca
     3961 tcaaggcgtg cctgaatgaa ttcgcgcctc gcggcctgcg cctgatcgaa gtcgacaagg
     4021 ccgatctggt cgatctgacg gacatcgtcg acctcgttgc cgagcgtcca gaacgcttca
     4081 tcgtctattg cgacgacctc agtttcgatg agggtgagcc ggggtacaag gctttgaagt
     4141 cgatcctcga cggaacggtg tcgcaggcat cggacaacgt ggtgatctac gcgaccagca
     4201 accgccgcca cctgcttccc gagtacatgg ccgagaacct gacctaccag cacctggaga
     4261 acggcgaggt gcatccgggc gaggtggtcg aggagaagat ttccctgagc gagcgtttcg
     4321 ggctgtgggt cagcttctat ccgttcacgc aggacgagta cctggccatc gtctcgcagt
     4381 ggctgcgcgg tttcggcgtc ggcgaatccg cgattgaagc ggcgcggccc gagagcctgg
     4441 tctgggcgct cgaacgcggc tcgcgatcgg gtcgggtcgc gtaccagttc gccaaggatt
     4501 tcagcgggcg cctgccgggg tgagtgcaga gcaccgaacg ccggtcgacg tggccgtggg
     4561 ggtcctgatc gatgcgcagg ggcgcttcct gatgacttcg cgccccgagg gcaaggtcta
     4621 cgctggctac tgggagttcc cgggcggcaa gctcgaggcg ggggaaagcg tcgaggaggc
     4681 gctgcgtcgc gaactgcacg aagagatcgg catcgtcatt ggcgaagcgc acccgtggca
     4741 ggtcgaactg gtcgactacc cgcacgcgct ggtgcggctg cacttttgca gggtgtacga
     4801 ctggcgcggt gaattcgaga tgcgcgaggc gcagacgatg tcctggcaga ccctgcccgt
     4861 cgaggtcatg ccggtgctcc ccggcacaat cccggtgctg cgctggtttg ccgaggagcg
     4921 ccaacatgcc ggtgcgaccc accgtggatg agcgcgctgt gctcagggtg aacccgattg
     4981 ctacactgct ccgatgagtt ggatcgacga tgtgaaatgg gatgcccagg ggctggttcc
     5041 ggtgatcgcg caggagatcg gcagcaatga tgtcctgatg ttcgcattca tgaaccgcga
     5101 ggccttgcag cgtacagccg acctcgggga ggccgtctac tggagccgct cccgcagtcg
     5161 tctgtggcac aagggcgagg agtccgggca catccagaag gtgcatgagc tacgattgga
     5221 ctgcgacaac gacgtggtgc ttattcgtgt ggagcaggtc ggtcacagtc ccgggatcgc
     5281 ctgccatacg ggacgacatt cctgcttcta ccagctttac cgcgatggcg cgtggcacac
     5341 cgtcgaaccg gttctgaagg atccggaaca catctacaaa tgacatctcc cgacgcaaca
     5401 ttacccctgt cgcaggacgt gctcgcacga ttggccgagg tcattgaatc gcgcaaggcg
     5461 ggcgacccgg acaagagcta cgtcgcgcgg cttttccaca agggcaccga ctccattctg
     5521 aagaaggtcg gcgaagaagc caccgaagtg gtcatggctg ccaaggacgg cgatccgcag
     5581 aaggtgatct acgaggtcgc cgatctctgg ttccactcga tgctggccct gagcgccttc
     5641 ggactgaagc cggccgacgt gctcgcggaa ctgcagcgcc gcgaaggact ctcaggtctg
     5701 gaagagttcg cggcccgcaa gccgatacag cgcgaaggag gggaaaaatg accgaagccg
     5761 tgttagccgg ccccgaagaa aagccgcatg gtggactgcg cacgctcacg cacatcctct
     5821 acgggctgca tctgctgtcg tggttctctg cgggcgcctt ctcgctggtt gccatggtga
     5881 tcaactacat caagcgcccc gatctgcccg atgaactctt tcgaagtcac tttcgctggc
     5941 aagcccgcag tttctggttc accctgctct ggctgaccgt cacctcgccg ctcttccttc
     6001 tgttctggat tcctggtgcg gcagcgtggt tcctgatcgg cttgtggtac ctctatcgtt
     6061 tcgtccgtgg ctggttgagc tttgccgaga gccgatcaat gccgcagccc tcctgacaaa
     6121 cgcacccgct ggatatgaat gctgatccca actgcatctt ctgcaagatc gtcgccgggc
     6181 agatcccgag ccgcaaagcc ttcgaagacg aggatgtcct cgtcttccac gacatccacc
     6241 cgtgggcacc ggtgcacctg ctgatcatcc cgaaggagca catcgcaacg cacgccgacg
     6301 tgacgccgga gcacgagcga ctgctcggca agatgctgtt gctggtgccg aagctcgcga
     6361 aggaacacgg cgccgacaac ggttttcgca ccatcatcaa caccggccgc gacggtggtc
     6421 aagaggtcct tcaccttcac atgcacgtga ttggcggtcc ccgcccctgg aaacagggct
     6481 gacctagaat ccgcggtcct ttcaaggaga gataaaaatg ggttcattca gcatctggca
     6541 ttggctgatc gttctggtga tcgtcgtgct ggtcttcggc acgaagaaat tgaagaacat
     6601 cggcgccgac ctgggcggtg cggtcaaggg gttcaaggac ggcgtgaagg acggcggtgc
     6661 ctctgcagac gccactgccc cggcgcagca ggtgacgtct caaaaatcgg ctgatgcaaa
     6721 cacggtcgac gtggaagcca aaaccaagtc gtgattgatt tcggcttcga caagctggcg
     6781 ctcatcggcg cggtggccct gatggtcatc ggcccggagc gccttcccaa agtggcgcgc
     6841 acggtcggtc acctgatcgg caagatgcag cgctacgtcg cggacgtcaa gtccgaagtc
     6901 aatcgttcga tcgaactcga cgaactcaag aagatgaagg gccagtttga agatgcggcc
     6961 cgcaacgtcg agcaaacggt gtccaacgag atccaccaga cgaactccga cctgcgcgac
     7021 agcgttgaag ccgacagagg cactgcagtg gacacgacat ctcccgactt caatcgatac
     7081 gacaccttgc tgaccccact tcccgactat cgcaacccga agaagcagtg gcggctcaag
     7141 cgcaccgcca tgccgcagtg gtacaagcag cgccacggca ttcggggcaa ggcgttgtcc
     7201 gggtccgccc gcgtggcgcg cttccgtcca cctcgctcgt cggcctgatt gatggcctcc
     7261 aatccttccg gccccgatgg actcggcggc gaacaacctt ttgtcacgca cctgatcgag
     7321 ttgcgagacc gtctggtccg cgcttcgatc gccgttggca ttgcattcgg cgtcctgtgc
     7381 ctgtggcccg ggcccggcgg gctctatgac ttgctggcag ctccgctggt ggccagtctg
     7441 cctaagggcg caacgctgat cgccaccaac gtgatctcgc cattcgtcgt gccgctgaag
     7501 atcacgctga tggctgcctt catgctggcg ctgccggtgg tgctctacca ggtctgggcc
     7561 tttgtggccc caggcctcta cacgcacgag aaaaaactgg tgctgccgct ggtggtgtcg
     7621 agcaccttgc tgttcctggt cggtgtggcc ttctgctact acttcgtttt cggacaggtc
     7681 ttcaagttca tccagagctt tgcgccgaag agcatcacgg cagcgcctga catcgaggcc
     7741 tacctcagtt tcgtgctgac gatgttcatt gcattcggtg ccgcgttcga ggtgccggtg
     7801 gtcgtcgtcg tgctggcgcg catgggcctg gtcacgatcg agaaactgaa ggaattccgc
     7861 agctatttcg tcgtgctggc cttcatcatc gcggcgatca tcacgccgcc ggatgtcgtt
     7921 tcgcagctgg ctcttgcgat cccgatgtgc ctgctgtacg agttgggcat ctgggccgct
     7981 cggatcttca tcaagcacac gcaggcgccg gtcgagaaag cgacaccgac cacgtgatgc
     8041 ttatcgtccc cgcgctgctg tgctcgccgg attcttcggg cgctgcgccg cggtgatctt
     8101 caagctcagc acctgattgc cgcgctgtac gctgatcgag gtggcagcct gcggtttcag
     8161 ggccgccacg gcattcagca actgggcggt gttgagaacg cggttgtctc cgacactgat
     8221 caccacgtcg cccggaagca ttccgccttc gctggccggg ccgttctgca gcaccccggt
     8281 gatcagcacg ccctgcttga ccgacaggtt gagcgtttcg gcgatctccg gcgtcaggtc
     8341 ccgcggctcg acgccaatcc agccccgggt gacctggcca tcctggatca gcccctccat
     8401 cacctggcgg gcaatgttgg ctggaatcgc gaacccgata cccatgttgc cgcccgagcg
     8461 cgagtagatg gcggtgttga tgcccagcag attgccgttc gcatcgacca gtgcgccgcc
     8521 cgaattgccg gggttgatcg ccgcatcggt ctggatgaag ttctcgaagg tgttgatgcc
     8581 gaggtgatct cgccccagcg cactgacgat gcccgaggtc accgtctgcc cgacgccgaa
     8641 tgggtttccg atggccagga ccacatcgcc gacttgcagt gcgtcggtgt tggcaaacgt
     8701 catcaccggc aatcggtcga gcgcgatctt cagcaccgcc acatcgcttt ccgggtcggt
     8761 gcccaccacg gtggccggcg cgctgcggcc atcgggcaac tggacgtcga tgtcgctagc
     8821 gccctcgatc acgtggttgt tggtcagcag gaaaccttcg ggtgaaacga tgacaccgga
     8881 gccgagcccg atctgggttt cctggcggtt gtcgcggtcg ccgaaaaaga attcgaacca
     8941 tggatcgccg gcgtgcggcg tgcgggtgga agtcttgctg gcagtgatgc tgaccaccgc
     9001 cggcgaagct cgcttggcgg ccgcggcgta gctgttggca tggctgctcg ccgagctggc
     9061 tgcgggagcc tcgctgatcg agaccatgtc cggcaggccc gctatcgaat gccgccgcag
     9121 ccactccggc ttcagcgtgg cgatcacgaa cagcaccgcc acggcaaccg tgactgactg
     9181 ggagaaaatg agccaggttt ttcgcatgag gttgagtctg tggcccgtcg ttccgaaatc
     9241 gattcgtatc tgtccgcgtt gctgcagacg gcgagcttca aggattatgg gccgaacggt
     9301 ttgcaggtcg agggccgcga cgaggtgcgg cgtatcgtgt cgggcgtcac cgccagccgc
     9361 gcgttgatcg atgcggccat cgacgctcga gccgatgcgt tgctggtgca tcacggactg
     9421 ttctggcgtg ggcacgacgg acgcgtcaca ggctggttga aacagcgcct tgaacggctg
     9481 ctggcgcacg acatcaacct gttcgcctac catctcccgc tggacgcgca tgccgagttc
     9541 gggaacaacg cgcagttcgg cgcccgcttg ggtgtggtgg ccgatgcgcg tttcggcgag
     9601 cagtctctcg gcttcatcgg ccagctggcc gagccgacca gcgttgcagt gctggttgat
     9661 aggatgctgc gcctgtcggg ccgcgcgcct gtctcgattg acggtgatgg ccggcccctg
     9721 gcacggctgg cctggtgcac cggcggggcg cagtcgtact acgaggcggc gatcgcggcc
     9781 ggggccgacg tcttcatcac cggagagctg tcggaaccgc aggcgcacct tgcccgcgaa
     9841 accggggtgg cgttcatcgc gtgcggacac cacgccagcg aacgctacgg cgcaccggca
     9901 gtgggcgggc atctggcggc gcagttcggc atcgagcaca tctacatcga gatcgacaat
     9961 ccggcctgat ggctgcagaa ccggctgccc ttcggggagt gcggtcacgg agatccattt
    10021 catgagagag cgcgctcagc acgctgcgac accgatcgtc atcacgatgg gcgacgcctg
    10081 cggcatcggc cccgagatca ttgcgaagct gtttcgcggc ccgtctgcgc agggttgctt
    10141 cgtccttggt gacgtcgagg tgatgcgcag agctgcgcgg ctcacagggg gattgctggc
    10201 gttggcgccg attgattcac cggacgcgct ggcagacgtg ccgccgaatt gcgtgccggt
    10261 actgcaggcc gatgccttgc cgcacgacct gctctcagcg ccgatcgggc gcatcgactc
    10321 gcgtgccggc gcagccgctg cccgctgcat cgagcaggct gtcgctctgg cgcttcgagg
    10381 cgaggtgtcg gccatcgtga cctcgccgat ccacaaggag gcgctggcag cggctggtgt
    10441 cggtttcccc ggccacacgg agatgctgca ggcgcttgcg gccgagcccg gcatgccgcc
    10501 gccgccggtg cgcatgatgc tggccaacga tgaactgcgc accgtgctgg tgaccatcca
    10561 tcagtctctt cgaagtgcca tcgatgcggt cacgttcgac gctgtgctcg acaccgtgcg
    10621 cattgcgcat cgggccgctg tgcgttgggg cctgtcgtct ccgcgaattg ctgtggccgg
    10681 cctgaatccg cacgctggcg agggcgggct gttcggtgat gaggagacca ggatcatcgc
    10741 gccggccatc tctgcggctc gggcggaagg catcgacgcg agcggcccgt atgcaccaga
    10801 cacggtgttc atgagggcgc gcaatgcctt gccggcacat ccgggggagt tcgacgttgt
    10861 ggtggcgatg acgcacgacc acggactgat ccccgtcaag tacctgggtg tcgagcacgg
    10921 cgtcaacgtg acgctcggcc tgtcattcgt gcgcaccagc ccggatcacg gcactgcatt
    10981 cgacatcgcg ggaagtggcg tggccgaccc ctcttcgctt gaggcagcca ttgcgatggc
    11041 gcgaaaactg gctaaagact gagagctgcc ccggtcgccc gcagtcgctg tatgcgatcg
    11101 gggcgacgtc tgctcactgc cgcgagttgg cggtcagtgc cgagagttgc ttgcgagccc
    11161 gggacgtcgc gcgctccatc aggtagttgc tttcgaccgc acgcaggcgc tcagcctcgc
    11221 acatgcgcga cagcatgcgc gcggtcagcg agatcgtctc ctgcaggttg cgcccgtgcg
    11281 tgaagacgaa gaagctgcgg cccgggctca tgtaggtcag acgtactttc tgccactggt
    11341 ccttgagcag catctggtag gcgaagccga tccgcaggtg atcgatcagg cgcgcgccgt
    11401 gggacgcctc ggcggggtct gcaccgacca ggtcgagatt gatgtcgacg cccaggtcca
    11461 gcggcgcgct ctccagcgtg ggcggtggct cgtggctgag gtcgatgtcg acctgttgcg
    11521 cccaatcgac ggttttctcg tctatcaggc cgacctgctg tgcttcctcg ggggtgaagc
    11581 gctgctcgat ctcggccgat gtcaggggaa cggaaaggcc cggatcggtg cgcagcgaat
    11641 cccctttttc aggcaccccg ctgttgaaga tcgactcgag ctgcttcgac agcatgttgt
    11701 agtcgagctc ggtcatcgcc ggcaacttga gcgattccgc gtgtgcgggc agcaactggc
    11761 cgaagaattc cttttgcgcc ccctcgggcc agcctatgag cttgaggcct tcgttcaagt
    11821 cgcgcatgag cgtgggcaac tgcatcagga agcgcttgcg cattcccgga gaacccttcg
    11881 gctgcacgct catcaccaga tcgcggccga cgttgcggta gcgcttggtc ggctcgccat
    11941 cgtcaccttg cttgcgcgcg gcctgaacca gcgcctggct ccagacctgg cacaggaagg
    12001 aacgcaggaa gtcgggcatc gagatcgacg acagcgctcc ctgcaactgg atcaggtacc
    12061 gctgctgcgt gcgcaggtcg gtttccttgt tctccagcac ggcaatcgct ccccgatcct
    12121 tgacctcgga ggtagcttgc tgcgtgatga acgattccag ttcgcccagc ttggccgcat
    12181 acagatcgac ctgatcgaag tcaccctcga cgatttcctg cacgagatca cggacacgct
    12241 cgacgaattc cttgcccggg ccatcatcga aatcgtcgaa tgcgcaagcc agcgcggcaa
    12301 tccggttgat gaatcggcgg accggatgac gacgcgacga gaagaaggtg gagtcgacca
    12361 gcgccacgcg cagcaccggc aattgcaggc gcgcgatctc acgggccatc tgcggcggaa
    12421 ctcgtgtgtc ggacaggatc tgatcgaaaa ggctgccgac gacatcgatc accatgtggt
    12481 cgagcttgcc ggtcgaggcc tggatcagtt cttcgcggtg agccctgatc aggttcaccg
    12541 ccatcaagcc ggccgatgcg tcgtctgcat aggcgctggc cggtcccggg tcggcgtagg
    12601 ggccggtcgc gatcccgcgc agtgcgggtt cggcggcata gcggctggcc ggagcgcttg
    12661 gcgacgtgct cccatgcaaa ccgctcggca aacccagttc gtctgaagag ttgctcagct
    12721 gtgtcaatcg acgcagcaag gtcatcagct gggcatcctg cgcaggcgct gccccgccac
    12781 ctgaaacatg ccccggcccc ccatagcccg tggtgggtcc ccgtgagccg ctgccatggc
    12841 ctgaaggtgc agcgtcgatt cgaccgctgc gcgtgccctg ccaactggtc gacccatagc
    12901 ccgaagaact gccgctgcgt gagcccgatg tcgtcgggcc ggaagtcgac ctgtgcgcgg
    12961 acagacctga gtgggatgag gtctgaaact cggagccgtg accgcgcgtc gatgacagtt
    13021 cgtcacgcag gctcgcgtag ccggaattga ttcccccggg caacgcgttg ccggggccct
    13081 ccacggtgcg cacctggaga ctcaccgggc tgatgcctct cgactgcatc tcgacgacga
    13141 ttgccgcgta gcacgccggc atggcctggg ccatgacgat gccgaactcg cgcgcgagca
    13201 gggcgagttc ggtcgggtcc gatgtcacgg cctcgatggc gaaatagagg gcctggccca
    13261 cgatggacgg gcgcaggggg ttgtgctcat cgtcggcggt gccgaatccc gtgatgcgcc
    13321 ccatgtagcc cgagagttcg cgcaattcga cttcgcacgc atgagcgatc tgctgcccca
    13381 ggcgatcgga gtgcatgcgc tcctcgactt ccttgtcgtc gaccagcgtc agcgtttccc
    13441 agtcagctgc atgggcatgg cgacgcgtct cggtgcgggg cgccagcgtc tcgagcatgc
    13501 gggcatccag ccgggtgcgg aaattcagtc gaaacggccc gagttgccga tgcagtgcca
    13561 gctgagcggc aaacatcgtg tttcgatcaa tcgccttcga agccgactgc gattgagcag
    13621 ccagggtctg acagacgcgc tccgcgactg tcaaggcagt ggccttgacg aggtctgccg
    13681 cagcttccag ctcggcaggc agtcgctgat cggtggggtt catctcgggg aaacgctaca
    13741 tccgccggag gttggagccg gtttgcaacg ctgagtatga gaccaaacca gcgcccgaac
    13801 gccgacgcgg gcgacgggcg atgattgcgg ccgcctactt cctcaatgcg tcacgaattt
    13861 cacgcaacaa gagcacgtct tctggtggcg ccgcaggcgc tgcagcgggg gcctcgggct
    13921 gctgctgctt catgcgattc acggccttga ccatcaagaa gatgatgaac gccaggatca
    13981 cgaagttgat cgcaaccgtg aagaagctgc cataggccag cacggcgcct tccttcttgg
    14041 cgtctgccag cgtggtcgcg gcctgcccgg acaggggaat gaagtagttc gcgaaatcga
    14101 gaccaccgaa gatcttgctg accaccggca tgatcacatc cgacacgagc gagtcgacga
    14161 tcttcccgaa cgcgccgcca atgatcacgc ccacggcgag atcaaccacg ctgcccttca
    14221 tcgcgaactc tttgaactct gacgcaaatc ccatgggttt gactcctgat tgttttggtg
    14281 gatgccgcgc ttccgcggcg gcgatggacc gcccgcggcc tcgccgcacg agtattgccg
    14341 atcgacgtgg cgggtcaatc cagggcagtg ctgctgtgtg tcttccaggg atggctggta
    14401 atcaagagtc gcccttgcat caggatggcg cccgtgcgac ccacatcgct ccgctagaat
    14461 ctcgggttgt ctgaaagtcg tcgttaaaac gcgaggtttt atgagtgagc agcaagtgga
    14521 cagtggcaag cgcacctggt tgatcgcatc gggttgtgct ggcgctgtgg gtggagtagc
    14581 cgttgcagtg ccgttcgtca gcacgtttca accttcggaa cgtgcccgcg ctgctggtgc
    14641 agcggtcgag gtcgatatca gcgcgctgaa gccgggcgag aagctcaccg tcgaatggcg
    14701 tggcaagccg gtctggatcg tcaagcgcac gccagagcag ctcgctgcgc tttcaaaggt
    14761 cgattcgcaa cttgccgatc cacagtcgct gcgaaagcct ggcgaactta cgccggaata
    14821 tgcgcgcaat gagcaccgct ccatcaagcc ggaaatcatg gttgcggtgg gtatctgcac
    14881 ccaccttggc tgctctccct ctgacaagtt cacgccgggc gcccagccgt cgctgcccga
    14941 cgactgggtg ggcggattcc tctgcccctg tcacggttcc actttcgacc tggccggtcg
    15001 ggtctacaag aacaagcctg ctcccgacaa cctcgaggtg ccgccttaca tgtacctctc
    15061 cgatacccga ttgctgatcg gcgaagacaa gaaggcctga tggccctgca gtaacggcac
    15121 aacaggagaa cacacgatgg ccactttcaa ggaagcaccc gctaacgcca acgcagccga
    15181 aaagctgttg acgtgggtcg acaaccggtt tccggcaacc aagctgtaca acgagcacct
    15241 tgccgactac tacgcgccga agaatttcaa tttctggtat ttcttcggct cgctcgccct
    15301 gttggttctc gtgatccaga tcgtcaccgg catcttcctg gtgatgcact acaagccgga
    15361 tgcggcgctc gccttcgcat cggtcgaata catcatgcgg gacgtacctt ggggatggct
    15421 ggtgcgctac atgcactcga ccggcgccag tgcattcttc gtcgtggttt acctgcacat
    15481 gttccgcggc atgatctacg gcagctatcg caagccacgt gaactcgtct ggatcttcgg
    15541 ttgcgccatc ttcctgtgcc tgatggccga ggccttcatg ggctacctgc tgccatgggg
    15601 ccagatgagc tactggggtg cccaggtgat cgtcaacctg ttctccgcca tcccgttcat
    15661 cggcccggac ctgtcgttgc tgatccgtgg cgactacgtc gtgagcgatg cgacgctgaa
    15721 ccgcttcttc agcttccacg tcattgccgt tccactggtg ttgcttggct tggtcgtggc
    15781 gcacgtgatt gccctccatg aggtggggtc gaacaacccg gacggcgtgg agatcaagtc
    15841 caagatgaac gccgccggca agccgctgga tggaatcccc ttccatcctt actacacggt
    15901 gcacgacgtg ttgggtgtca gcgtcttcct gctgatcttc tcggcaatca tcttcttcgc
    15961 tcccgagctg ggtggctatt tcctcgaata caacaacttc atccccgctg attccctgaa
    16021 gacgccactg cacatcgcac cggtctggta cttcacaccc tactactcga tcctgcgcgc
    16081 gatcacggcc gagatgatgt acgtgctgat cgggttcgtc gtgctaggcg cggcgctggc
    16141 agtcttcaag gtgaagatgg gcgctctgct caagctcgtg gttgtcgcag tagctgcggc
    16201 cgtggtcgcc atgatgctgt cgatcgacgc caagttctgg ggtgtggtcg ggatgggggg
    16261 cgctgtcgtg atcctgttcg tgctgccttg gctcgacaac agcctggcga agtcgatccg
    16321 ttatcgcccc gattggcaca agtacgtctt tgcccttttc gtcatcaatt tcctgatgct
    16381 cggctatctc ggcacgcagc cggtgtcgcc aaccggcgaa cgtctgtcgc aggttggcac
    16441 ggtgatctat ttcggcttct tcctcttgat gccatggtgg agtcgcctgg gtgagttcaa
    16501 gcccgttccg gatcgcgtga cattcgcagc ccactgatgg tctaggcagg ctttaaaaaa
    16561 tgaaaaaatt catcatcaca ctgctgctct ccgttgcagg tttgtcggtt gcgaatgcaa
    16621 acaccggtgg catcgcctgg gataagtttc cggtcgagaa ggcggcggac gtcaccgcct
    16681 tgcagaacgg cgcgcgaacc tttgtcaact actgcctgaa ctgccacaac gctgcgttca
    16741 tgcgctacaa ccgactgaga gacatcgggc tcacggagga tcagatcaag aagaacctga
    16801 tgttcgcgac cgacaaggtt ggtgacgtca tgaaggtgac gcttgatccc aagaacgcca
    16861 aggattggct gggcgcggtt cccccggacc tgacgctgat cacccgctcg cgatcagcgg
    16921 cagggcaggg cacgggtgca gactacgtct acaccttcct gcgtaccttc taccgtgacg
    16981 agacgaaagc caccggctgg aacaatcttg ctttccccaa catcgggatg ccgcacatcc
    17041 tgtgggagtg gcagggacag cgcagcgcga agttcgttga ggaagccgat ccgcacgacc
    17101 agaccaagaa ggtccatcgg tttgccggct ttgagcagat cacgccagga acgaaatcag
    17161 aggccgaatt cgatcaggcg gttggcgatc tggtggccta cctgaactgg atggccgaac
    17221 cggcgcaggg cacccgcacg cgcctcggtg tctgggtgtt gcttttcctc gcggtgttca
    17281 cgttcattgc atggcgactc aacgcagcat tctggaaaga catcaagtga tgcttcgcct
    17341 gcccggcctc cctggtggat gtcgggcaac tgcgcagtgg gtcagcccgg aacggcgccc
    17401 cctgcgtttt ttctttctcg atcccttgag gagctcgccc acatgatggt gctttactcc
    17461 ggcacgacct gcccctattc acaccgctgc cgattcgtgc tgttcgagaa aggcatggac
    17521 ttcgagattc gcgacgtcga cctgttcgcc aagcccgaag acatcgcatt gatgaatccc
    17581 tacaacgagg tacccatcct cgtcgagcgc gatctcatcc tgtacgagtc gcacatcatc
    17641 aacgaataca tcgatgagcg ctttcctcat ccgcagttga tgccgggaga cccggtggcc
    17701 cgtgcccgtg tccgactgtt cctgctgaat ttcgagaagg aactgtttgc acatgtgaac
    17761 ctgctggagt cacgcggcgt gaaagccacc gacaagcagc tcgagaaggc cagggcacag
    17821 attcgcgatc gcctcacgca actcgcgccg atcttcctga agaacaagtt catgctcggt
    17881 gacgacttct cgatgctcga cgtggccatc gcgccgttgt tgtggcgcct ggactattac
    17941 ggcatcgaca tgtccaagaa tgcggtcccg ctcctgaagt acgccgagcg tatcttttcc
    18001 agacccgcct acatcgaagc cctgacgcca tccgaaaagg tcatgcgcaa gtgatcttgc
    18061 gtggttgcca ccctctcgac cgttactgat gacgctgccg cccacccccc ccgtgcttgg
    18121 caacagcggg acatccacgc gcccgtatct gatccgggcg ctgcacgagt ggtgcaccga
    18181 caacggattc acgccctaca ttgctgtctt cgtcggaccg gatgtgaagg tgccgatgga
    18241 gtacgtgaag aatggcgaga tcgttctgaa tgtgggtttc gaagccacca cggcgcttga
    18301 gttgagcaac gagcgtatcg agttcaaggc acgcttcggt ggcacgtcgc gtgacatctc
    18361 cgttccggtc gatcacgtca ttgccatcta cgcgcgagag aacgggcaag gcatggcgtt
    18421 tccggtaccg gccgacgcgg cagcgcctgc cggatcgggc ggaggaccgc gcgttcagcc
    18481 acaggaaccc gccgagcggg tcggcatggt gctgcgtctc gccgcagagg gtgacgaacc
    18541 ggctgcagtg ccagctgctg cgcctgcacc ggcgggtgat gacccgccgt ccgaaccccc
    18601 gcccgcaggc ggacgaccgg cgttgaagcg ggtcaagtga tggtggcgtt cgtgccgacg
    18661 gcacgccgtt aaaatttcca gacgcccttg tagctcagtt ggtagagcag cggttttgta
    18721 aaccgaaggt cgcgggttcg attcctgccg ggggcaccag atttgcgtga tcggctcggc
    18781 gttcaatgcg tcggctgcgg tccgttcttg gcacttcagg ctgcccggac cttgatgcgc
    18841 accgcaaggg atggaaagcc tcgtctcagc aactgttcct ccagccgcgg ttgaaggtgt
    18901 cgcaatttgg ctgccacgtt ggcattggcc gcgatcagcg tccagccttc gtcgtcgatc
    18961 ggtcccggcg ccacatgagc catgagccca ggtggtatgc acgactggat ggcggcaaag
    19021 cgtctggcgg attcatccag gcggcgccga agcgaagcga gcggattgct gcgcctcagt
    19081 gcctctccga gagtcagggc gtccggcacg gatggggcgg ctttgctcat gctggtttcg
    19141 agtgtaggtc gcgtatccgg gactgcttcg agagatcggt tcgtcgccat gcctgacgga
    19201 gacacgatgc ccggcggtaa actcaggagc tttgctgtag cgcgctggcc cttggcctcg
    19261 ccagcagcga gtgcctctac ggtcgaggcc acccgaattc accctcagcg cgcagtggtc
    19321 ggcctaccgg tccggctgcc acgacgccct acccagtgac gccgcatgtt gcccaagata
    19381 ctcacccaaa ttttcggcag tcgcaatgaa cggctgctca agcagtaccg ccgcgttgtt
    19441 gttcaggtca atgcgctcga accgaaattc gaggcgcttg atgacgcagc gcttgctgca
    19501 aagaccgtcg agttccggca gcggcacgtg gccggagaaa gtctcgcagc cttgttgccg
    19561 gaggcgtttg ccgtcgttcg cgagggcagc aagcgttcac tgaagatgcg tcatttcgac
    19621 gtgcagttga tcggcggcat gacgcttcac gacggcaaga tcgccgagat gcgcaccggc
    19681 gagggcaaga cgctgatggc cacgctgccc gtctacctga atgccatcgc caacaagggc
    19741 gtgcatgtgg tcaccgtcaa cgactacctg gccaagcgcg atgccgagtg gatggggcgc
    19801 ctctacaact tcctggggct gagtgtcggg gtcaatcttc cgcagatgca gcgcgaggaa
    19861 aagcaggccg cctatgcagc cgacgtgacc tacggcacca acaacgagtt cggcttcgac
    19921 tacctgcggg acaacatggt ccaggaggtc agcgatcgcg tgcagcggca gttgtcttac
    19981 gcgatcgtcg acgaggtcga ctcgatcctg atcgatgagg cgcgcacgcc gctgatcatc
    20041 agcggccagg ccgaagacca caccgagatg tatgttcgca tcaatgacgt ggtgccgaag
    20101 ctcaagaagc agatcggcga ggctgatccg cgaaccggcg agggcgtcat cgagccaggc
    20161 gacttcacgg tcgatgaaaa gacgcatcag gtctacctga ccgaggatgg tcacgagaac
    20221 gccgagcgcc tcctcagcga agccgggctg ctcgccgagg gggccagcct gtacgacgcc
    20281 gccaacatct cgctgatgca ccacgtgtac gccgcgttgc gtgccaatca cctctacaac
    20341 cgcgaccagc actacgtcgt gcagaacggg cagaacgggc ccgaagtcgt catcgtcgac
    20401 gagttcaccg gccgcctgat gaccggccgc cgatggtccg acggcctgca ccaggcggtc
    20461 gaggcgaagg aaggcgtgcc gatccagagc gagaaccaga cgctggcatc gatcaccttc
    20521 cagaactact tccgcatgta cggcaagctg tccggcatga cgggtaccgc cgacaccgaa
    20581 gcctatgagt tccaggagat ctacggcctc gagacggtgg tgattccgcc caatcgtccg
    20641 accgtgcgca aggacgagct cgacctgatc tacaagagca gccgagagaa attcgaggcc
    20701 gtgattgccg acatccgcga gtgccatgaa cgcgggcagc cggtgctggt cggcaccacg
    20761 tcgatcgaga actccgagct gatatcgggc ctgctgcaga aggcgggcct gccgcaccag
    20821 gtgttgaacg ccaagcagca cgccagggaa gccgacatcg tcgctcaagc gggtcgcccg
    20881 ggtgtgatca cgattgccac caacatggct ggtcgcggca ccgacatcgt gctcgggggc
    20941 aatgtcgaga accagatcaa gtcgcttcaa gccgatccga ctattccgga ggccgagcag
    21001 ctgagccaag cccggaagct gcatgaagag tggcagggcc tgcacgaccg cgtgaaggcc
    21061 gaagggggcc ttcgcatcat cgcgaccgag cgtcacgagt cgcgccgcat cgacaaccag
    21121 ctgcgtggtc gttcgggacg tcaaggggat ccaggttctt cacgcttcta cctgtcgctc
    21181 gatgattcgc tcatgcgcat cttcgccggc gatcgcgtgc gttcgatcat ggagcggttg
    21241 aagatgcccg acggcgaggc catcgaggcc ggcatcgtca cgcgcagcat cgagggtgcg
    21301 cagcgcaagg tcgaggcgcg caatttcgac gttcgcaagc agctgctcga gtacgacgac
    21361 gtcgccaatg atcagcgcaa ggtcatctac cagcagcgca acgagatcct cgaagcccag
    21421 agcctgatgg agcagatcgg cagcctgcgc cgcagcacga tggccgacgt cgtgcgaacc
    21481 ttcgtgcccg ctgaaagcct ggaggagcag tgggatctcg acgcgttgga gaaggtgctg
    21541 cgcgaggagt ggcaactcga gctgccgttg aaggccgagg tcgaggccag cgcgtcgatc
    21601 acggacgacg acatcgtgga gcgcgtcgcg aaggcggccg atcagctgtt cgatgccaag
    21661 gtcgagatgg tcggtgtcga ccagttcacg ccgttcatcc ggatgatctt cctgcaatcg
    21721 atcgaccagc actggcgcga gcacctggcg gcgctcgact acctgcgcca gggcatccac
    21781 cttcgcggct atgcgcagaa gaacccgaaa caggaataca agcgcgaggc cttcgagttg
    21841 ttcagccagc tgctggacgt gatcaagatg gacgtcacgc gcaccttgat ggcggtgcgc
    21901 attcgttcgg ccgaggaagt ggcccaggcc gccgaggaaa tcgagcaacg cgcagagcgc
    21961 atcgccaacg tcacctacac ccaccccaac gaggacggca gcttgtcgac cgaatcgcag
    22021 caggccatga ccgatcccgc catgcaggtg gcacaggaga tgggcgagcg cgctgaggcc
    22081 cctgccagct tcgctcgggc agggcgcaac gatccttgcc catgtggcag cggcaagaaa
    22141 ttcaagctgt gccacggcaa actggcttga ggtatccaac atgcctgtca acctgaaagc
    22201 gccgaatccc caagacttga agcccgtgcc tggcgtgcgg ctcggcaccg caatggccgg
    22261 ggtgcgcaag gccaaccggc gcgacgtgct cgtcgtcacg ctggccgaag gttcgcaggt
    22321 ggcgggagtg ttcaccagca accggttctg cgctgcgccg gtacaggtgt gccgtcagca
    22381 tctggcggtc gaccgccgtg cagcagcggg tatccgggcg ctgctcgtca acaccggcaa
    22441 cgccaatgcc ggcacaggcg aagatggtct ggcccgtgct cggtcgacct gtgtcgcgct
    22501 gtctggccgc ctcggcctgc agcccgatca ggtgcttccg ttctccacgg gcgtgatcat
    22561 ggaaacgctg ccgaacgacc gcatcgaagc aggcctgccc gccgcgctcg ccgatctgga
    22621 tgcggaccac tgggccgctg cggctgaagc catcatgacc accgacacgc tgccgaaggc
    22681 ggcttcggtt caggtccaga tcggcggcag gacgatcacg gtcaccggta tcagcaaggg
    22741 tgccgggatg attcgcccga acatggcaac gatgctcggt ttcgtcgcga ccgatgcggt
    22801 gctcgcgccc gggctgctgg acaccctggt gcgtgaagcc gccgactgct cgttcaaccg
    22861 catcacgatc gatggtgata cctcgaccaa cgattccttc ttgctgattg ccacgcagca
    22921 gggcggccac ccgccgatca cgcaactcga cagtggcgac ggagccgtgc ttcgtgacgc
    22981 cgtgatcgca gtgtccacga cgctggcgca ggccatcgtg cgcgatggcg agggcgcgac
    23041 gaagttcatc gcggtgcagg tcgacggcgg tgccaccgaa gccgagtgcc ggctcgcggc
    23101 ctatgcgatc gctcattcgc cactggtcaa gacggcattc ttcgccagcg atccgaatct
    23161 gggtcgcatc ctggccgcgg tgggctatgc aggaatcgcc gatctcgacc agacgttgat
    23221 cgatcttcac ctcgacgacg tgcatgtggt gcacaacggt gggcgtcatc cggcctatcg
    23281 cgaggaggat ggccagcggg tgatgaagca aagcgaaatc acggtgcgcg tcaacctgca
    23341 ccgcggcgcg gccagcacca ccgtctggac ctgtgatctc agctacgact acgtgaagat
    23401 caacgccgat taccggagtt gatcaggcgc tgactcgatc tctccggtgg cctgccttgg
    23461 cggcaggcgg tccacggcat cggtgatcag ggcatccacc ccgcatttga aaagccagtc
    23521 cgccagcgcg gcatcgttga cggtgtaggc cagcaccagc catccctcgg catgggcccg
    23581 ttcgatcagg gccgcgtcca gcagcgggtg acgtgccacg accgccttgc atgcgagtgc
    23641 ccgggcctgt tcgaaccacc cgggagtcag cgtgtccagc agcagtgcgc gcggcaatcg
    23701 gggggctgcc tcgcgcgccg ccgcgagtgc gtccgcagaa aacgaactca gcaacggaag
    23761 acgtcgttgg cccacccaca gtcgggctgc ctcgctggcg acccgcgctc cagtgagcgc
    23821 ttccagcccg ggtgtcggct tgatctcgat gttcagcagc gacccgctgt cgatgcagaa
    23881 ccgcgcgatg gtggcgaggc gaggtatcga ctcgccctcg aagggcgcgc tgtgccagcg
    23941 accagcatcg agcgtcgaca gttcggccca ggttcgatcg ccggcgatgc ccgtcgtgcc
    24001 ggtggttcga tccagtgtgg cgtcgtgcat caggaaagcc acgccatcgg agctgagctt
    24061 gacgtcgcac tcgaaggccg tgtagccgaa gcgagctccg gcgcggaagg cggccagtgt
    24121 gttctccggg gccaggcgtc ccgccccgcg gtgcgccagc caccttgtgg actgccaggc
    24181 ggcgatggca tcgaggctgc aactgtcggt ggtcatgctg tgtgcggcgt ggtggcgttc
    24241 gagagggtgg gcccgtccgg gccctgacga tagacccctt cgtcgccaga gcgatgtggg
    24301 gccactctgg ttttcccgag gccagcggcg acaatcggag atctgcgcaa cgcgcccgcg
    24361 tcgacttcgt gccctcgggt ccggtcgacg tccgagaccc tacatgaatg cacccacgac
    24421 cctcaccgaa ctgacgtcag cggcccgcgt cgaaaccggc acgcctcgcc tgcgcgagat
    24481 tccctacaac tacacctcgt tctccgaccg cgagatcgtc ctgcgcctgc tcggttcgcg
    24541 ggcctgggaa ctgctcggcc gcctgcgcgg cgagcgccag accgggcggt cggcgcgcat
    24601 gctctacgag gtgctgggcg atatctgggt cgtgcagcgc aatccctatc tgcaggacga
    24661 cctgctggac aacccggggc ggcgaagcct gctgatcgag gcattgcacc accgcctgaa
    24721 ggagatcgag aaccgcgcga caccttcgga tgggcaccat catggcgatg tcagcgagtt
    24781 gctggcctgt gccaaggccg cggtgcgacg ctttgccgat cagttcgaag aggttgcctc
    24841 tctgaggcgc agggccaccc ggaccctgcg tcgctgcacg gcggctgaca acgtcaagtt
    24901 cgatgccctg tcgcgcgtct cgcacgtcac cgatgcgacc gactggcggg tcgagtaccc
    24961 gttcgtcgtg ctcactccgg ataccgaggc cgagatggct gcgctggtga agggctgcgt
    25021 cgaactgggc ctgacgatcg ttcctcgcgg cggcggcacc ggatacaccg gcggtgcgat
    25081 cccgctgacg tggaacagcg cggtcatcaa caccgagaag ctcgaggcga tgagcgaggt
    25141 cgagatgcgc gagctgcccg gcgtcgatcg gcctgtcgcc acggtatgga gcgaagccgg
    25201 tgtcgtcacg cagcgcgtgg ccgatgccgc cgagcgcggt ggcttcgtgt tcgcggtcga
    25261 cccgacctcg gccgaggcga gctgcatcgg cggcaatatc gcgatgaacg cgggcggcaa
    25321 gaaggccgtg ctgtggggca ccgcgctcga caacctcgcg tcgtggcgca tggtcacacc
    25381 cgaagccaag tggcttgagg tcacgcggct gaaccacaac ctgggcaaga tccatgatgt
    25441 cgaggtggcc agcttcgagc tgaagtactt cgacgccagc ggcaagacgc tggagcgcac
    25501 cgagcggctc gatatcccgg gcgggacctt ccgcaaggaa ggtctgggca aggacgtcac
    25561 ggacaagttc ctggccggtc tgccgggcgt ccagaaggaa ggctgcgacg ggctgatcac
    25621 cagcgcgcgc tggatcgtgc acaagatgcc tgcccatgtg cgcaccgtgt gcctcgaatt
    25681 cttcggcaat gccaaggacg cggtgccaag catcgtcgag atcaaggact atttgttcgc
    25741 gcaggcgaag gccggcggcg cggtgctggc ggggctggag catctggacg accgttacct
    25801 gaaggcggtt ggctatgcga ccaagagcaa gcgcgctcat gcgggtggaa acacgtcggg
    25861 tctgcccaag atggtgctgt tcggcgacat cgccggcaac gatgcggacg cggtggcccg
    25921 tgccaccagt gaagtgatcc ggatcgccaa cagccgcgcc ggcgaagggt tcgtcgcggt
    25981 gagccccgaa gcgcgcaaga agttctggct cgaccgcaag cgcaccgcgg cgatcagcaa
    26041 gcacaccaac gccttcaagg tgaacgagga cgtggtgatc cccctggggc ggatgggcga
    26101 gtacacagac ggcatcgaac gcatcaacat cgagctgagc ctgcgcaaca agctggcgct
    26161 gctcgatgcg ctggaggagt tcttccgcaa gggtcagttg ccgctcggca agggtgacga
    26221 cgccgtagac atcccgtcgg ccgaactgct cgaagatcgc gttcagcagg cgttgtcggt
    26281 ggtcgaggag gtgcgggcgc tctggcagca ctggatgacg cacctggaca tcagtgacgg
    26341 tgagcgcagc tatttcgccc cactgcagga ccgcacgttg cgcgcttcgt ggaagctgca
    26401 ggtgcgtgcg cccttgcagg cgatcttcgc cggcgccgcg ctggcgccga tcgtcgaggc
    26461 ctgccagaag atccacaagg acgtgctgca cggccgcgtc tggatcgcgt tgcacatgca
    26521 cgccggcgac ggcaatgtgc acaccaatat tcccgtcaac agcgacgact acgacatgct
    26581 gcagacggcc catgaggcgg tcgcgcgcat catgaacctc gcgcgtcgtc tcgacggcgt
    26641 gatctccgga gagcacggga tcggcatcac caagctcgag ttcatgaccg acgccgagct
    26701 ggccgatttc gctgcgtaca agcagcgtgt ggatcccgaa ggccgcttca acaggggcaa
    26761 gctgttgcgc gggggactcg ggcgctcagg ggcggtcgac gtcggcacct cgtccgaggt
    26821 gcggccttcc gacctgagcc aggcctacac gcccagcttc gggctgatgg gccacgagtc
    26881 gctgatcatg cagcagagcg acatcggcgc catcgccgac agcgtcaagg actgcctgcg
    26941 ctgcggcaag tgcaagccgg tgtgcgccac ccacgtgccg cgcgcgaacc tgctgtacag
    27001 cccgcgcaac aagatcctgg cgacctcgct gctgatcgag gccttcctgt acgaggaaca
    27061 gacgcgccgc ggcatcagcc tgcggcactg ggaggaattc gaggacgtgg ccgaccactg
    27121 caccgtgtgc cacaagtgcc tgagcccgtg cccggtcaag atcgacttcg gcgaggtgtc
    27181 gatgaacatg cgcaacctgt tgcgcaagat gggcaagaag agcttccggc cgggcaatgc
    27241 ggtggcgatg accttcctca acgcgaccag tccccagacg atcaacctga tgcgcggtgc
    27301 gatggtcggc atcggcttca aggcgcagcg gctggccaat gacctgctgc agcgcctggc
    27361 gcgcaagcag accgcgttgc cgccggccac cgtgggcacc gcgccgttga aggaacaggt
    27421 catccacttc atcaacaaga agctgcctgg cggcttgccc aagaagaccg cgcgtgcctt
    27481 gctagacatt gaggacaagg actacgtccc gatcatccgc aacccgcaga ccacgacgtc
    27541 cgagacggag gcggtcttct atttccccgg ctgcggttcg gagcgccttt tctcgcaggt
    27601 cgggctggcg acgcaggcca tgttgtggca cgccggggtg cagacggtgc tgccgccggg
    27661 ttacctgtgc tgcggctacc cgcaacgggg cagtggccag ttcgacaagg ccgacaagat
    27721 catcacggac aaccgggtgc tgttccaccg cgtggccaac acgctgaact atctcgacat
    27781 caagacggtc gtggtgagct gcggtacctg ctacgaccag ctgcagggct accagttcga
    27841 caagatcttc ccgggctgcc gcatcatcga catacacgag tacctgctcg agaagaacat
    27901 caccttgcca ggccagggcg gctacctgta tcacgatccc tgccacagcc cgatgaaact
    27961 gcaggagccg atgaagaccg tcaaggcgct gctgggcgcg aacgtgatcg atgccaagcg
    28021 ctgctgcggc gagagcggca cgctgggcgt gacgcggccc gacatcagca cgcagatccg
    28081 cttccgcaag gaagaggaat tgcgcaagga cgaggccgcg ttgcgcagga ccggtgcggt
    28141 ggccgagact ggcgcgatca aggtactgac ctcatgcccc agttgcctgc aggggctgag
    28201 tcgatacacg ggcgacctgc agaacggact gctcgaggcc gactacatcg tcgtcgagat
    28261 ggccaaccag ctgctcggcc ccgactggat gccgcagtat gtggagcgtg cgaaccaggg
    28321 tggcatcgag cgggtgctgg tctgatgcac gcagggtgtc cgttgtgcga ggctgcgggc
    28381 ggggtgcggg tggcgcaggc cgcgcgctgg cgtgtcattc gcgccgagga tcccgcgttt
    28441 ccagcgttct accgggtggt ctggactgaa catgtcgccg agttcagcgc gctgtccgct
    28501 gcgcaccgct cggagtgtat ggacgtggtc tgcgcggtcg agcaggcgct gatcacctcc
    28561 ctgcagccga cgaagatcaa cctcgctgcg ctgggaaatg tggtgcctca cctgcattgg
    28621 catgtgatcg cacggttcga ggcggacagc cactttccgc agccgatctg gggcccgcag
    28681 cagcgagagt gtctcagccc gatggccctg cttcgcgtcg ggcttgccga actggacgat
    28741 cgtgtggctg cggcggtttc cccgatttgt cagcgcttgt gacagcgtcc gtgcagcctc
    28801 accccttcgc tacactgacc tgctcgatca tgcaggcccc gtaacaccat ggctggactc
    28861 accgccgaca cccccacacc caccgcagtc accgtgcatc agcaatcccg tgtgcttcag
    28921 atcggatttg cggacggcag cgagttccgc atccctttcg aactgatgcg ggtgtattcg
    28981 ccgtccgccg aggtccaggg acacggacct gggcaagaag tgctgcagac cggcaagcgg
    29041 gatgtcgaac tggttgaact cgaaccggtc ggcaactacg cggtccagcc gaccttctcg
    29101 gacggccaca actccggcat cttttcctgg gactaccttt atttcctggg gtctcagcag
    29161 gccgatctgt ggaaacagta cgaagagcgc ctcgccgcgt cgggaactga ccgcgacgcg
    29221 ccgatggcag cggccgcggg cgcttcctgc ggcagccact gacttcacac cggacacccc
    29281 cgtgagcaac acccatttcg gattcaagtc cgtcgaagaa tcccagaagg ccagtcgcgt
    29341 tcgtggcgtt ttcgattcgg tggcatcacg ctacgacgtg atgaacgacc tgatgtcgat
    29401 gggcctgcat cgagtctgga aggcgtacac gatcgccgtg gcgaacgcgc gcctgggcga
    29461 tcgcgtgctc gacatcgctg gtggcacggg cgatctggct ttggcattca gcaagaaggt
    29521 cggacccggc ggtaccgtcg ttcacaccga catcaacgaa gcgatgctgc ggcaggggcg
    29581 cgaccgcctc accgatgccg gcgtgctgct gccgacggcg atctgcgatg ccgaggcgct
    29641 gccgtttccg ccggccacgt tcgatctcgt gtcggtggca ttcggactgc gcaacatgac
    29701 gcacaaggac cgcgcgctga ccgaaatgaa ccgggtgctg cgcccgggcg gccgcttgct
    29761 ggtcctggag ttctccaggg tggccaagcc gctcgaaaag gcctacgact ggtattcgtt
    29821 caatgtcctg ccgaagattg gacagtgggt cgccggcgat gccgacagct accgttacct
    29881 ggccgagtcg atccgcatgc accccgatca gggcgccctg aaggcgatga tgaagtccgc
    29941 cgggttcgcg cacgtcgacg tgcacaacct cagcgccggt gtggtggcgc tgcacgtggg
    30001 catcaagtgc tgaccgtcag aacatcgcgt cgactcgcac gctgaacctc tggagaaacc
    30061 gcgaatgatc tcgaatctcg acaagcccga ttccagccgc gacgaccgga agcccggccg
    30121 cctcgtgctc ggcgcacttc tggtctcatt tgcggccctg gtcggatgca accccaagca
    30181 agaggaagca accgcctctc agccggcggc caaggtgaac aaggaggaga tcaccgtggc
    30241 gcagatcaac ttcgtgctgc agcagcaacg tggtctgaag ccggaacaga ccgaggccgc
    30301 gagcaggcag atcctgcaac gcctgatcga ccaggaactg gctttccaga aagcccagga
    30361 agtcgggctc gatcgggatc cgcgcgtact ccagctgctc gaggcggctc gtcgcgaaat
    30421 cgtcgcgcgc gcctatgtcg agaaggccgg gcaggcggct gccaagccgt ctgccgacga
    30481 tgtcaagaag tactacgacg agcatcccgc gctgttcagc gcccgccgca tctacacgtt
    30541 gcaggaagtg gtgatcgaga ccaagccaga gcaattgcct gccttgcgag aacagctagg
    30601 ggccgcccag accatcagcg agttcatcga gtacctgaag gccaacagct tccgcttcgc
    30661 tggaaatcag gccgttcgga ccgcggagca attgccgctc aacatgctcg atgccatcgc
    30721 gaagatgaat gacgggcagg cgatgctgct gcagaccgct gtcggtgcgc aggtcatcgt
    30781 gctggccggg tcccggtccg aaccggtcga ccaggcacgc gccacgccag tgatcgagca
    30841 gttcctgttg aacgacgcca agcgcaaggc agctgaagcc gacatcaagg ccctgagagc
    30901 cgcggcgcag atcgagtacg tcggtacgtt cgccagcccg gcggcgagtg cgcctgctgc
    30961 gggtgaacct ctgccttccc tgacagcgcc ggcctcggcg gcgcaggatg gcacgtcact
    31021 gccgcttgaa gccgaatcca tcggcaaggg catgggattg aaatgatgca gctccgggcg
    31081 gtgcggtccg tgctcgccat cgtggctttc ggagctgctc ttgcaggcag cccggcgttc
    31141 gccgcacagg tcggcggggg atccgcacca gcggcatcgg ccgcggcgga ataccggctc
    31201 ggggccggcg atctggttcg tgtcaatgtg taccagaacc ccgatctgtc gctcgagacg
    31261 cgcatcggag aagcgggcaa caccagtttt ccactgctcg gcagcgtgaa gatcggcggc
    31321 ctggtggtct ctgccgccga aaggctgatc gctgacggtt tgcgcaatgg cggcttcgtc
    31381 aagcagcccc aggtcagcgt cctggtgctg caggttcgtg gcaaccaggt gtcggtgctg
    31441 ggtcaggtga atcggccggg ccggttcccc atcgaggtgg ccgacatgcg gttgtcggac
    31501 ctgcttgcca tggccggggg tatttcggcc attggcgccg acacggtgat cctggtcggt
    31561 gcgcgcgatg gcaagcctat tcggcgtgag ctcgatctgc tctccatctt tcgcagcgcc
    31621 agccgggacg aggacatcgt cgtgcagaac ggcgacgtga tctatgtcga gcgcgctccg
    31681 acagcctaca tctacggaga ggtgcagcgt gcgggtccga tgcgcgtcga gcgtggcatg
    31741 acggtgatgc aggccctcgc cacgggaggc gggctgaccc agcgcggcac cgaaaaaggc
    31801 ttgcgcatcc accgcagggc agcggatggc agggtccaga tcgtccagcc cgccatggac
    31861 gatgccttgc gcgacggcga cgtggtctac gtcaaggaaa gcctgttttg accggatcgc
    31921 ctcaggcgcg tcctatcccg gagtactcga tgcccagtgc gcgcatcagt gcgggttcgt
    31981 agaggttgcg gccatcgatc acgactggct gtttcagcat cgatttgatg gcgtcgaagt
    32041 ccgggctctt gaactccttc cactcggtga tgatgaccag cgcatcggac ccctcgagtg
    32101 cctgcgtctg gttgtcgacg aaatcgagac cgcgcacgcc ttcgagcacg cgcctggcct
    32161 cggtcatggc gaccgggtcg taggccttga ccttcgcacc gcgcttggcg agttcggcga
    32221 tcacgacgcg gctgggcgcg tcgcgcatgt cgtcggtgtt gggcttgaat gccaggcccc
    32281 atatggcgaa ctggcgaccg ctcaagtctt ctccgaagcg cttgacgatc ttgtcgacca
    32341 gcacgtgctt ctgcttttcg ttggcggcct ccacggcggt cagcacctgc agggtgacgc
    32401 cgttgtcccg agccgtgtga accagcgcct tcacgtcctt cgggaagcac gaaccaccat
    32461 agcccgcgcc ggggtaaagg aagtgcgtgc cgatgcgcgg atcgctgccg atgcccttgc
    32521 gcacgagttc gatgtcggca ccgacccgct cggccagcag agccagctcg ttcatgaagc
    32581 tgatccgggt ggccagcatg gcgttcgcgg cgtacttggt gaactcggcg ctcttgatgt
    32641 ccatcaccag caggcgatcg tggttgcgca tgaagggcga gtagatcgat cgcatcagca
    32701 ggatggcgcg ttcgtcgtcg gcgccgacga cgattcgatc gggacgcatg aagtcctcga
    32761 ccgctgcccc ttccttcagg aactcggggt tcgagaccac ggaaaactcg agttcgaccc
    32821 cgcgcttggc cagctcttcc gccacggccg cgcggacctt ctcaccggtg cccacgggca
    32881 ccgtgctctt gtcgacgatg accttgtagt cggtcatgtg ccgacccacg ttgcgcgccg
    32941 cggcgaccac gtactggaga tccgccgagc cgtcttcgtc cggcggggtg cccacgccga
    33001 tgaattgcag ggtcccgtgg ttgactgccg cgaccacgtc cgtggtgaac tgaagtcgac
    33061 cggcggcacg gttgcgcttc acgacgtcca gaagtcctgg ctcgtggatc ggaatgccac
    33121 cgtcctcgag gatcttgatc ttggcggcgt cgaggtcgag gcagaccacg tggttgccca
    33181 tttctgccag gcaggcgcct gtgacgaggc ccacatagcc ggtgccgatg atggtgattt
    33241 tcatggggtg ttcatttgga attgaatcgg aatcggtcta ctttgcgcgc ccgtaggtgt
    33301 cctcgaaccg cacgatgtcg tcctcgccga gatagctgcc ggattgcacc tcgacgatct
    33361 ccagcgggac ttttcccggg ttggagaggc ggtgcgcctg acccagcggg atgtaggtgc
    33421 tctggttctc ggtcaacagg atcaccgaat caccgttggt gacctcggcg gtgccccgca
    33481 ccacgatcca gtgttcggcg cgatggtggt gcatctgcag gctcagcgtg gcgccgggct
    33541 tgaccatgat ccgcttgacc tggaagcgcg agccggaatc gatgctgtcg taccagcccc
    33601 agggccggtg cacctgccgg tgcagggtgt gttcactgcg accgtcctgc tgcagctggt
    33661 tgacgagctg tttgacatcc tgactcgatg accgccgggt gaccagaacc gcatcgggcg
    33721 tctcgaccac gatcacatcg tcgagaccga tcacgccgac cagtcggctg gtggcatgca
    33781 cgagggtgtt gcggcagtcg cgcagcatcg cgtcgccgtg gctcgcgttg ccgttgccgt
    33841 ccttggtgct gacttcccag acggcatccc aggcgcccag atcggaccaa ccggcgttca
    33901 tcggcaccat gcggatgtcg aactcgggcg acccgggaca acgctccatc accgcgtagt
    33961 cgacggattc gctgggtatc gcctcgaatg ccgcacggtc cggtcggatg aaactggcat
    34021 cggtggttcg tagctcccag gctgcccgcg tggctttgga gatgtcgggc ctgaactgct
    34081 ccagcgcctt cagccacacc gatgcccgca ccacgaagat gccgctgttc cagtagtagc
    34141 ggccatcggc aaggtagcgc tcagcagtgg cacggtcggg cttctcgaca aactccgcga
    34201 caacctgcgc aggactcgcg ctttgactgc cggcagcctc gcagcgcagg tagccgaaac
    34261 cggtctctgg accggtcggc tggatgccca acgtcacgat cgcgccttcg cttgctgcgc
    34321 gcactgcact ttgcagcgcg cgggtgaatc cgccttcgtc gacgtaggcg tgatctgcag
    34381 cggtcaccac caatatcggg tcggctccgt cagcggtcgc gtgcagggcg gccagcgtca
    34441 tggcgggcgc ggtgttgcgg cccatcggct ccagcaggac cgaggccggc ggaagcttca
    34501 attcgcgcag ttggtcgagc acgaggaagc ggtgctcctc gttgccgaca acgaggggcg
    34561 gggccaccgc gatgtcggtc gtggccagtg aggccaggcg ctgtgcggcc tgctggaaga
    34621 ggctctggtt gcccttgagc accaggaact gcttcgggtg ctgcgcacgc gacagcggcc
    34681 acagacgcgt gccgctgcca ccggccatga tcacgggatg gacggaaatg gagctcatgg
    34741 gcagtcagtt caagtggatg tcgacggcgg aaccaggcgc gcccccggga aggaccgcgc
    34801 gatgctggcg tgatttcaat cggtgtatcc gttcgggttg agactttgcc accgccatgt
    34861 gtcagtgcac atcgtgtcga tgtcacgggt cgcctgccag cccaaatatt cacgcgccag
    34921 cgtggcgtcg gcataacaag ctgccacgtc gcccggtcgc cgggccacga tgtcgtacgg
    34981 aatgggcctt ccgctggcct tttcaaaggc gcggaccagg ctcaggacgc tgcatccctg
    35041 gcccgtaccc aggttcaccg tcagcgatcg tccgccgccg tcgagcaggt atcgcagcgc
    35101 tgccacatgg ccttcggcca gatccgtgac gtggatgtag tcacgcacgc cggttccgtc
    35161 cggggtgtcg taatcgttgc cgaacacctg caaccgcggg cgcttcccga cggccacctg
    35221 gctgatgtag ggcatcagat tgttgggcgt gccgcgaggg tcctctccga tcagcccact
    35281 ctcgtgcgcg cccacgggat tgaagtagcg cagcagcgcg atgcgccagc tggggtcggc
    35341 gcgctcgata tcgcgaagga tgttctcgcc catcagcttg gtggcgccgt agggattggt
    35401 ggtcgacagt ggcgagtctt cagggatcgg caggcgctcg ggcgtcccgt acaccgtcgc
    35461 gctggaactg aataccagct tcttcgctcc ggattgcgcc agcgatccag ccacgctgaa
    35521 caggccgccg aggttgttcc ggtagtaggc gagcggctgg gcgctgcttt caccgacagc
    35581 cttgaatgcc gcgaaatgaa tcaccgccgc gatggtgtgg cgctggaaaa ccgatgccat
    35641 cgaagcggtg tcgcacacgt cggcacgctc gaaattggcg gaacgccccg acagtcgctg
    35701 cagtcggttc aggacctcgg gcgagctgtt ggagaaatcg tcgacgccga cgacatcgaa
    35761 tcccgcagcc cagagtgcga gccaggtgtg ggaagcgatg tagccggtgg cgcctgtgag
    35821 cagaacggtg cttctcattg cagcttgatc tccgcggtgc atgtggctga actggcgctg
    35881 tagccgaacg atagcgagcc tgcggtgctg cgcgtctcgc ggccgacttg gcaggagaac
    35941 ttcaaatggc gcaagggctc gtacgtcacg ccgaggctgg ccgcgcgcaa tgtgtcgctg
    36001 ccgtcgatcg aaccggcgac aagcgagcga tggttggcgc gaactccggc atcgacgtgt
    36061 attttcgcgc tcagcgcgta cgccgcactg acagccagcg aattggtgat ccggcttgca
    36121 tcgccgacgc ttccgttctg ggcttcagcc acgtcgagga aactgctttc ggccccgctt
    36181 tcccggctga acgctgtctt gaagagcagc ttgccggtcg gccgataggt ccagatcagc
    36241 tgtccggtgg cgcccgagaa gtcgagcgcc gaggtggcgc ccctggactc gcgtcgaccg
    36301 acgctgaacc gcccgtcgag cgtgctgagt cccgtggcca gccacgtcgc cgtcaggtcg
    36361 atgtcccggc ggtcgaaatc gagacccgaa cccttgtatt cgccgcgact gagtcgcaat
    36421 ccggtgccca gtctcaaacc cgcactgggc tggtaataca gagccattcc ggccgtgtcc
    36481 tgggtcaagg cgctggcctc gtaggccggg ttcgaatacc gcaggctgcg gtggctcagc
    36541 gaggcctcga ccgccaggtg cgaacccgag ccgtacctga tcctggccaa gctctcgctg
    36601 cgtgtttcga tattgcgctc ggtcgattgg gcggtgttgg tgccaccgta gttgaagagg
    36661 ccatccgcgc cagaaaagct cagcgttccg gaaagcttgt ctatggctgc ccagtcgagt
    36721 ccggcgttga tcgcatagct cgtgtggtcc agtcggctca agtcctggaa gcgcgtttca
    36781 cgcaacctga cgttggcaaa gaggcgttgg cggccgatcg gttgatccag gccggccagc
    36841 aggccgacgc tgtaggaggt gtccgaggtc gcctttttca ggcgaaagac gttgctgtca
    36901 tgcaccgccg agagatcggc accgacgtag aacggattgg cttctgcgtg tgccggtccg
    36961 gcgagcggcc acatgagcat ggccagcatc aggcctcgcg ctggaagggt caaatttgag
    37021 cggcgcttct gcaaggtggg aggactccgg ctgcgggcga ggcgctgtgc ctgtgcgatt
    37081 caggttcggg ccgggctcgt gcgtcgatta aacctgcatt gcaggcgttc atagggaaaa
    37141 ccagccccga caggtgcatc agtttgtgag cgccgggttt gtccgacctg ctcggatgtt
    37201 ccaggatggc aagcgcgcgg gtaccagatg catgtccggg tcgcggcaag gcaactggtc
    37261 gccatgccta caatcccggg atgctcagcg cgctgaatgc gggcatcgct gctgctgtca
    37321 cggcacgagc gaccctgctc atcaatcatg tcctgtcctc cgagccggtg gcttgcggtc
    37381 ggttgcgtgc gcatgccggc cgccggcttc gcatccgatt gctcgattgg cccggtttgc
    37441 tgcccgcttt gcctgcgttg tgcttcgcca tcaccgccgc ggggctgctg gagtggcaac
    37501 ctgaggtcga tggggctggc cctgattcgc aggtcgacct cgacatcacg gtcgaagccg
    37561 gcaacccggc ggcattgctg ttcgggggtt tggtgggcca gcgccctgca gtgaccattt
    37621 cgggggactc ctcgctggcg gccgacgtca gctgggtgat cgacaacctg cgctgggacc
    37681 tgcgcgacga cgtggctcgc ctcatcgggg aaatcccggc acgcgagctg gcgcgccagg
    37741 cttccgccat ggccgctggc ctgcgcggcc tggtgcagga cgtggtccag cgcgccacat
    37801 cggctcgagg cggtgttggc ggtgcagcgc agtccccgtc accggcacag cggccggcgc
    37861 gatgaggcat ctggcccgac tcctcttcat cgtcttcacg gtttatcgat acgggctcga
    37921 cgagctggcg ctgtcgaatt tccggcagcg ctgggtccgc agcctggtcc gactgggcac
    37981 gctggggcgt cgtctggacg cgccccgcgg cgagcgcctg cgccttgcgc tggagcgact
    38041 gggcccgatc ttcgtcaagt tcggtcaggt gttgtcgaca cgccgcgatc tgattcctcc
    38101 tgacattgcc gacgaactgg cgcagttgca ggacaacgtg ccaccgtttc ccgcagcgct
    38161 ggcgcggcag atgatcgaaa aggcctatgg ccgaagcatc gaggacgtct tcgccacgtt
    38221 cgaggcggag ccggtggcca gcgcgtcgat cgcgcaggtc cacttcgcca cgctgaagga
    38281 cggtcgggag gtcgcggtca aggtgctgcg gccgaacatg ctgccggtga tcgacgacga
    38341 cctgtcgctg atgcgcacga tggcggtctg ggtcgagcgc ctgtcggccg acggcaagcg
    38401 gctgcggccg cgcgaggtgg tcgccgagtt cgacaactac ctgcacgacg agctggatct
    38461 ggtgcgcgag gcagccaatg ccgcccagct gcggcgcaac atggacgggc tgaacctggt
    38521 catgctgccc gagatgcact gggagctgtg cacccagaca gtcatcgtga tggagcgcat
    38581 gcacggcgta ccgatcagcc agacgcagcg gctgcgtgac gccggtgtcg atattcccaa
    38641 gctggcgcgc gacggcgtca cgatcttctt cactcaggtg tttcgtgacg ggttcttcca
    38701 cgccgacatg catcccggca atatccaggt cagcctggcg ccggccacat tcgggcagta
    38761 cattgcgctc gatttcggca tcgtcggcac gctgaccgag ttcgacaagg aatacctggc
    38821 gcagaacttc atcgcgttct tccgccgcga ctacaagcgt gtcgccgagc tccacgtcga
    38881 atccggctgg gtgccgccga atacccgcgt cgatgagctc gagggcgcga tccgcgcggt
    38941 ctgcgagccc catttcgacc ggccgctgaa ggacatctcg ctggggcagg tgctgctgag
    39001 gctgttccag acctcccgcc gcttcaatgt ggagatccag cctcaactgg tgctgctgca
    39061 gaagacgctg ctcaacgtcg agggcctggg tcgccagctc gatcccgagc tcgacctgtg
    39121 gaacacggcc aagccattcc tggaacgctg gatgaacgac cagattggtt ggcgcgggct
    39181 gatctcgcgc ctgaagaacg aggccccgcg cttcgtgcaa ctgctgcctg aactgccccg
    39241 gctgctgcat cagtcgctgc agcccaagcc ggtcgccgag cagcggacgc tggaagcgtt
    39301 gctggccgag cagcggcgca ccaaccggct gctgcaggcc atcgtctacg ccggcctcgg
    39361 cttcctgatc ggcgtgctgg tcgttcagtg gttcatgcat gtcggctacc gctgaggcgg
    39421 cgccgtataa ttcgcgtttt tccttttcta atcaaaggct tagccatgcc gatctatgcc
    39481 taccggtgcg aggcctgcgg tcacgcgaaa gacgtgctcc agaaaatttc cgatccgctg
    39541 ctgaccctct gcccggcatg cggggcagag agcttcaaga agcaggtgac ggcggccggc
    39601 ttccagttga aggggtcggg ctggtacgtc accgatttcc gcaacggatc cggcggaggt
    39661 gcgccgtccg acaagaacgc aggtgattcc aatccggccg gtgagggtgc cggcaagggg
    39721 gctgcatccg gggccaagga gggctcagcc agtccggatg cggccggcag cccttcctcg
    39781 ccggcgcccg ctgccgcgcc agcagcgccg agtcccgcgg cgagcggctc atccagcgcc
    39841 ccgtcttctt catcgacctg agcggcggca gacgacgtca ccaccccgga ttcccaagga
    39901 accccttgaa gaaatacctc ctcaccggtc tgctggtctg gctcccgctc accatcacca
    39961 tctgggtcct gacctcggtg ctgggcgtgc tcgacgggct ctttgcctgg ctgatcagcg
    40021 cgtcccaggc ggtacttccg gccgctgcgc actcgacgct ggaaatgctc aagcagatcc
    40081 ccgggctggg cgtgatcgtc atgctggtcg ggctgctgct gaccggcatc ttcgcgacca
    40141 acatcatcgg ccaatggtgg ttgggtcagg gcagccgcct gctcaaccag attccgatcg
    40201 tcaagtcgat ctacagctcg gtcaagcagg tctccgacac gcttttctcc agcagtggca
    40261 acgcttttcg cgaagcggtg ctggtccagt atccgcgcca gggagcctgg accatcgcct
    40321 ttgtgacagg tcggcccggc ggcgaagccg gcttgcacct gccgggcgat tacctgagcc
    40381 tgtacgtgcc caccacaccc aacccgacct cgggcttctt cctgatggtg cctcgcgccg
    40441 atgtggtccc cttgaagatg agcgtcgacg aggccctgaa gtacgtgatc tcgatgggcg
    40501 tggtggcgcc accgacggca ccagcgtcgt cgcccccgga gcgaaatcag cccggttgag
    40561 cgtgacgccg gcgttcaacc ttccagtccc ggccagcgtg cattccacgc tcgcccaccc
    40621 accgatcccc gtgcgcttgt cgccaccgcc gtcaaggcgg ccacacatcg aattcgtttc
    40681 cggagtgaca ccatgagaac aacgtactgc ggcctggtca gcgaggcctt gatgggccag
    40741 accgtcacgc tgatgggctg ggcgcatcgc cgccgcgacc atggcggcgt catcttcatc
    40801 gacctgcgcg accgcgaagg cctcgtgcag gtggtctgcg atcccgatcg cgccgagatg
    40861 ttcaggacag ccgaaggcgt gcgcaacgag ttctgcctga agatcgtcgg caaggtccgc
    40921 gcacggccca ccggcacgga gaacgccaac ctgaccagcg gcaagatcga ggtgctctgc
    40981 ctcgagctcg aggtgctgaa cccgagcgtg acgccaccgt tccagatcga tgacgagaac
    41041 ctgtcggaaa ccacgcgcct cacgcaccgc gtgctcgacc tgcgccgccc ggcgatgcag
    41101 cgcaacatga tgctgcgcta ccgcgtcgcg atggaggtgc gcaagttcct cgacgtcaac
    41161 ggcttcatcg acatcgagac gccgatgctc accaagagca cgcccgaggg cgcgcgcgac
    41221 tacctggtgc ccagccgcgt gcacgacggc cagttcttcg cgctgccgca gagcccgcag
    41281 ctgttcaagc agctgctgat ggtggccggc ttcgaccgct actaccagat caccaagtgc
    41341 ttccgcgacg aagacctgcg tgccgaccgc cagcctgaat tcacccagat cgacatcgag
    41401 acctcgttcc tgaacgaggc cgagatccgg gcgatcaccg aggccatgat ccgcaccgtg
    41461 ttccgcaaga ccatgggcgt ggaactgccg gtgtatgccg agatgaccta tgccgaggcc
    41521 atgcacaagt acggctcgga caagcccgac ctgcgcgtca agcttgaatt caccgagctg
    41581 accgaggtga tgaagacggt cgacttcaag gtgttctcgg gcccggccac cacgcccggt
    41641 ggccgcgtgg cggccctgcg cattcccggc ggtgccgaga tgagccgcgg cgagatcgac
    41701 gcctacaccg agttcgtcaa gatctacggc gccaagggcc tggcctggat caaggtgaac
    41761 gacgccggca agggccgcga tggtctgcag agcccggtgg tgaagaacct gcacgacgcg
    41821 gcgattgcca agatcatcgc ccgcaccggc tgctgcaatg gcgacctgat cttcttcggc
    41881 gccgacaagg ccaaggtggt gaacgacgcg ctgggcgcgc tgcgggtgaa gatcggccac
    41941 agcgacttcg gccgcgagca cgggctgttc aacgacgtgt gggcgccgct gtgggtggtc
    42001 gacttcccga tgttcgagta cgacgacgac gccaagcgct ggaacgcggt gcaccacccg
    42061 ttcaccgcgc ccaaggacgg ccacgacgag ctgatgaaga ccgaccccgg cgcctgcatc
    42121 gccaaggcct acgacatggt gctcaacggc tgggagatcg gcggcggctc ggtgcggatc
    42181 caccgcgccg acgtgcaggc caaggtgttc tcggcgctca acatcacgcc ggaagagcag
    42241 caggtgaagt tcggcttcct gctcgacgcg ctgcagtacg gcgcgccgcc gcacggtggc
    42301 ctggccttcg gcctcgaccg cctggtgacg ctgatgacca aggccgagag catccgcgac
    42361 gtgatcgcct tcccgaagac acagcgtgca cagtgcctgc tgaccggcgc gccgagcgcc
    42421 gtcgacgaga agcagatgcg cgagttgcac atccgcttgc gcaacgcgca ggctgcgccc
    42481 cagaacgcgt cctgagccgg ccatggcccg cagcaaggag cgcgctccgg cggtgttcga
    42541 gttgcgcagc gcctcgatga cgctggtcgt cgtcgcgctg aagaccacgg acatggcggc
    42601 catcgaggcc gagatgacag ctcggttttc cgacacgcct gacatgttca accacgaccc
    42661 ggtggtgatc gacatcgctc aggccgaggg cgatccgatg gcgctcgact acggtcggct
    42721 cgtctcgctg cttcggcgct tcaacatggt ggctgcggcg gtgcgcggcg gctcgcctga
    42781 acagatgtcg gccgcggccg aggccggact gggtgaggcg cccgaagcgg tggtgcctgc
    42841 agcccgtccg gtggtgcagg ccccggctgc cgtcgaggcg ccgcccgtgg cagtcgaagc
    42901 ggtcactgca tcgccgatcg aagtcagccc gattccagct gcgccggccg tcccccagct
    42961 ggctcccgcg cacgttgcca cgatgatcgt ggacaagccg ctgcgatcgg gtcagcagat
    43021 ctacgctcgt ggcggcgatc tggtggtgct ggcggtggtc aatttcggtg ccgaggtgat
    43081 cgccgacggc agcatccacg tctacgcgcc gttgcgtggc aaggcgattg ccggggctcg
    43141 cggcaacacc gatgcgcgca tcttcagcac ctgcatggaa cccgaactga tctcgatcgc
    43201 tggcacctac cgcacgaccg acaacccgct gcccgacaac gtcatcggca agccggccca
    43261 gatccgactc gatggcgagc ggatggtgtt cgagccgctc gactgacatt ccccacccgt
    43321 ttctctctca cttcatctcg ttcgccccag gcgacaccac atcaacaagg accgatttcc
    43381 atgtccagaa tcatcgtcgt gacctccggc aagggaggcg tcggcaagac caccaccagc
    43441 gccagctttg cttccggact tgcgctgcgc ggacacaaga ccgccgtcat cgacttcgac
    43501 gtcggcctgc gcaacctcga cctgatcatg ggctgcgagc gccgcgtggt gtacgacctg
    43561 atcaacgtga tccacggcga ggcgaagctg aatcaggccc tcatcaagga caagcagtgc
    43621 gacaacctgt acgtgctggc ggcatcgcag acgcgcgaca aggatgccct gacgcaggac
    43681 ggcgtcgagc gcgtgctgga agaactcgcc ggcatggact tcgagtacat cgtgtgcgac
    43741 tcgccggccg gcatcgagac cggtgcgctg atggcgatgc acttcgccga cgaggcgctg
    43801 gtggtcacca accccgaagt ctcttccgtc cgcgactccg accgcatcct cggcatgctg
    43861 cacagcaaga cgcgccgcgc gatcgagaag aaggaaccga tcaaggagca cctgctgatc
    43921 acccgctaca acccgaaccg ggtgctcggc ggccagatgc tgagcctgaa ggacatccac
    43981 gacatcctgc gcgtgcgcat gatcggcgtg atccccgagt ccgaatcggt gctcgacgcg
    44041 tcgaatcaag gcgtgccggc gatccacatc aagggttccg acgtgtcgga ggcctacaag
    44101 gacgtggtca cgcgtttcct cggcgaggaa acgccgcttc gcttcgtcga tgcggcgaag
    44161 ccgagcttct tcaagcgcat gttcgggggg aagtgaacga tgggtttcct ttccttcttc
    44221 ctgggtgaga aaaccaagac tgccagcgtc gccaaggagc ggctgcagat cattctggcg
    44281 cacgagcgca acggccgcac gcgcgatccg gcctacctgc ccgacctgca gcgcgagctg
    44341 cttgcggtga tctcgaagta cgtgtcggtg ggaccgggcg acatcaaggt cgaggtcgag
    44401 cgccagggcg atctcgaggt gctgcaggtc aagatcgagc tgcccgaagc ggcgcgctga
    44461 cgccgcacga tcgatgaacg attcgcgccc gccgaagatc cccgagtcgg tgctggtcgt
    44521 gatccacagc gccgacctgc aggtgctgct gatcgagcgc gcggacaagc ccggcttctg
    44581 gcagagcgtg actggctcga aggacgcggt ggacgaaccg ttcgcggaga cggctcggcg
    44641 cgaggtcgcc gaggaaaccg gcatcgtgat cggttcggcc gacgtgccgc ttgcgcatct
    44701 gcgagactgg ggcatgcgca atgtctacga gatctacccg gtatggcggc atcgctacgc
    44761 gccgggtgtg acccacaaca ccgagcacgt gttcgggctt ctggttcccc gcgacatccc
    44821 tgtcacgctg gcgccgcgcg agcatgtgcg cagcgagtgg ctgccctggc gcgaggcggc
    44881 ggatcggtgc ttttccccat cgaatgccga ggcggtgctg caactgccgc ggttcgcagc
    44941 cgcgtgagcg ccgtgccgaa ggcagacttc ggtgcatcga tgcacccgtc agacgtcctg
    45001 cgcgtcgcga cctacaacat ccacaagggc gtgcgcgggg tcggcccgag gcgccggctc
    45061 gagatccata acctggcgct gggcatcgag gcgctggatg ccgatctggt cttcctgcag
    45121 gaggtgcgca gctccaaccg tgccgaggcg ctgcagtttc ccgacacgca catcggctgg
    45181 ccgcgcatgc cgcaggccga ttacgtcgcg cccgagggct acgccgtcgc ctaccgcacc
    45241 aacgcgatca cgcgccatgg cgagcacggc aatgcgctgc tgtcgcgctg gcccatcggg
    45301 gacatccgcc atcacgacgt gtccgatcac cgcttcgagc agcgcggcct gctgcacgtg
    45361 ccggttcaat ggaacggcac cttggtgcat gcgatcgtcg cgcacctcgg cctgatgcac
    45421 gccagccgcg ttcgtcaggt cgaacgcatc gccgccttca tttcgctgca tgtgccgcct
    45481 ggcgagcacc tcgtgctcgc cggtgacttc aatgactggg gcgagaagct cgatgcaccg
    45541 atccgccgca tgggtctgac gcctgcacat gccgccggcg ctcctccgcg aaccttcccg
    45601 tcgcgcgttc ccttcttctc gctcgaccgc gtctacacgc gtggcctgcg ctgcatcgcg
    45661 acgcacgtgc ctcgcagcgc ggcctgggca cgcatgtcgg accacctgcc gctggtcgca
    45721 gagttgcagc tcgtctgacc cgaggctccc ggccgatggc gaccgacgca tccagcgcac
    45781 gcgccgccgc cgatcgctcg gcgctggagc agttgtgggg cgcggtgcca cggccggtgt
    45841 tcagtggcgg caacgccgtg cgcctgctgc gcggcggcga ggaactcttc cccgcactgg
    45901 tcgaggccat cgacgaggcg cgtcacgagg tctgcctggc cacctacatc tttcacgacg
    45961 accttgctgc cgacagcgtc acgcaggccc tgatccgggc ggcgcagcgc ggtgtgcagg
    46021 tcaaggtcgt gctcgacggg ttcggcatgc tggggcgtct ttccgaactg cagcgacgtc
    46081 tgaccgaggg tacggtgcag gtggccgtgt accggccgct cgaccgctgg tggaactggt
    46141 tgcagcccgg gcaactgagg cgcctgcatc tgaagctgtg cgttgccgat gcacagatcg
    46201 ccttcgtcgg cggcatcaac ctgatggacg atcggttcga cctcaaccac ggctggaccg
    46261 aagagccccg gctcgatttc gcggtccgcg tcaccggccc ggtggtcgga ccgatccacc
    46321 aggcgaccat cgcagcgtgg acccgcgcgc atctcggcag cgccttcaag gaggaagcgc
    46381 tggcgatcgt gcgtggtgcc gagccgatgg ctcgcgtgcg caggctgatc aggcggctgc
    46441 ggttgagccg gggccagggc atgcaggtca caccgggcgt gccgagcccg atgcaggcgg
    46501 ccttcgtgct gcgtgacaac gtgcgtcagc gccgcaccat cgagcgcagc tacatcgacg
    46561 cgatcgcgaa ggccggcaag cgcgtcgacc tggtctcgcc ctacttctat cccgggcgat
    46621 cgttccgccg cgccctgaag cacgcggcgc agcgcggcgt ggcggtgcgg ctgctgctgc
    46681 agggcaaggt cgactaccgc atcgccagtc tggctgcgcg tgccctctac gacgagttgc
    46741 tggggcaggg cgtgcgcatc ttcgagtaca cgccggccta tctgcacgcc aagatcgccc
    46801 tggtcgatga cgattgggca acggtcggca gttcgaacat cgatccgctg tcgctgctgc
    46861 tgaacctcga ggccaacgtc atcgtgcgcg atggcgattt caatgcgcag ttgtcggcgc
    46921 agttcgacgc agctgtcgcg gtctctgacg agatcgatcc gaagctcaag taccgcaccg
    46981 gtgtgctggg tgcgctgcgg cgggcggttg tcgcctgggt cgcctacatc tacctgcggg
    47041 ttgccggggc gacaggccgt tattgatgtc tgtctcccgg tcgctgcgcc ccatccagcg
    47101 cggggccctg cctgtagcgg tgcgacccgg cgcccggcgt atcccccgat cgcgtcgctg
    47161 cagcggccat gacctcggct gacctcgtcg tcgagcgccc ggccggcctg tattgcccgc
    47221 cgggcgactt ctacatcgac ccgtggcggc cggtggatcg ggcggtgatc acccatgcgc
    47281 atgccgacca cgctcgctcg gggcatggcc actacctggc gtcagcgcgg tcggcgggcg
    47341 tgctgcgctc gcggttggga gccatcacgc tccagccgct ggcctacggc gaggtggtcg
    47401 agcaccgcgg cgttcgcatc agcctgcacc cggctggcca tgtcctcggt tcgtcccagg
    47461 tgcggctcga gcatggcggg cgagtctggg tggcgtcggg cgactacttc gcaaccgccg
    47521 ctgcgggcgc gccgggtgac gaggacaacc tcacctgcga gccgttcgag ccggtgcgct
    47581 gcgactgctt catcaccgaa agcaccttcg gtctcccggt ctaccgatgg cgcccgcagc
    47641 gcgaggtgta tgccgacatc aacgcctggt ggagcgccaa tgccgaggcc ggtcgcgcca
    47701 gcctgctgat gggctacagc ttcggcaagg cgcagcggct gctggccgga ctcgactcgc
    47761 gcatcggccc gatcgtcgtg catgccgcag tcgagccgct caaccgcgcc taccgcgccg
    47821 agggcgtgca actgccggtc acccagttgc tcggcgaggt gaccgaccgg gccggcctga
    47881 ggcgagccat cgccatcgcg ccgccctcag cccaggacag tgcctgggcg cgagcgttca
    47941 ccgtcggtgg ggtcgaggcc agcgatgcct ttgccagcgg ctggatgcag ctgcgcgggg
    48001 cccgtcgccg gcgcgccgtg gaccgcggct tcgtgatgag cgaccacgcc gactggccgg
    48061 gactgcagcg cgccatagcc gccaccggcg ccgatcgggt catcgtgacc cacgggtatg
    48121 aggcggtgat ggtgcgctgg ctcgaacagc agggcctgca ggcgggctcg ttccgaaccg
    48181 aattcggccg cgaggtcggt gccgaggagg cgtccgggcc acttcctgag ggcacggcca
    48241 gtggcgtcga aggatcggtc gccaagccgg acggggcggt gccttgaggc gcttctccgc
    48301 actgtttgcc gagctcgatg cgacgaccgc gacctcggcg aaggtcgacg cgctgaagcg
    48361 ctacttcgcg tcggctgagc cggccgacgc tgcctgggcc gtttatttcc tgtcgggcgg
    48421 caagccgcgc cgggtcgtgc cgacggccgt gctgcggtcg ctggcctgcg cgcgcgcggg
    48481 catcgacgac tggttgttcg acgagtgcta ccaggcggtg ggcgatctgg ccgagaccat
    48541 cgcgcatgtg cttgcgccac cttcgaggca cagcgatctc gggctggccg tctgggtcga
    48601 gcatcggttg ctgcccctgc gcggggagcc tcccgagacg caggccgccc ggatcgccgg
    48661 ctactgggac gaactcgatg ccgccgagcg attcctgctg atcaagctga ttggcggggg
    48721 cttccgggtc ggcgtcagca agctgctggt gcagcgcgca ctggctgaat cggtcggtat
    48781 cgacgccagg gtggtggcgc agcggatgat gggctacacc gaccaggctg cgcgccccga
    48841 tgcggccggc tacacgcgcc tggtgtcact cgacgccgag cccggccgcg atgccggaca
    48901 gccctatccg ttctttcttg cacatccggt cgacgacgcg gtgctggccg acttcgacgc
    48961 agcgctgggc cccgctggcg actggctcgt cgagtggaag tacgacggca tccgtgcgca
    49021 ggtggtgcgg cgtgccgggg aggtgtggat ctggtcgcgc ggggaagaac tggtgaccga
    49081 ccgattcccc gaggtggtca ctgccgcgca ggcgtggtcc gacggcacgg tgatcgacgg
    49141 cgagatcctg gcctggcttc ccgacagtga ccggccggcg cccttcaacc tgctgcagca
    49201 gcgcatcggt cgcaagaacc tgacgaagaa gatcctcgcc ggcgcaccga cgaccttcat
    49261 ggcctacgac ctgctcgagc acgccggtgt cgatcttcgc gaagcgccgc agcacgagcg
    49321 gcgggcggcg ctggaggcgc tcggcgctgc gatccctgcg ctgcgtctgt cgccgctgct
    49381 gcgcggcagc tggcccgaac tggctgcgct gcgcgagcag tcacgcgctc ggggtgtcga
    49441 aggcttcatg ctcaagcatc gcgaggcgag gtacggcagc gggcgccgca agcaggtggc
    49501 cgctctgccg ggatcgccct caccggcctg gtggaaatgg aaggtcgacc cgctgaccat
    49561 cgatgcggtg ctgatctacg cgcaggccgg ccacggccgc cgcgccagcg tctacaccga
    49621 ctacaccttc gcggtgtgga gccgagcgcc gcgcgatgcg gccgaagcac aggcggtcgt
    49681 cgatgccatc gaggcacggc agccggcgca gcccgacggc ctgcagctga tcgccttcgc
    49741 gaaggcctat ggcggcctga cggacgagga gttcaaggcg gtcgaccgcg tgatccgcca
    49801 gaccacgctc gagaaattcg ggccggtgcg cagcgtgcgc ccgacgatgg tgttcgaact
    49861 gggcttcgaa ggcctcaaca tcagcactcg ccacaagagc ggcatcgcgg tgcggtttcc
    49921 caggatgctg agaatccgcg gtgacaagcc gctgcgcgaa gccgacaccc tcgatacgct
    49981 gcgagcgctg atgaaccctg gagaccctgc cgatgagcac gacgaacacg accccggcga
    50041 cggactttga ctgcgacctg ctggtgatcg gcgccggcag cggtggtgtt cgtgccgcgc
    50101 gtttcgcggc gcagcgcggc gcccgcgtga tcatcgtcga ggggggaccg ctgggcggca
    50161 cctgcgtcaa cgtcggctgc atcccgaaga agctctacag ctatgcggcg caccacggcg
    50221 aggccttcga gcaggcgcac ggcttcggct gggaagcgac cacgccgcgc ttcgactggg
    50281 ccgtgctgaa ggcgcgccgc gccgccgaga tcacgcggct gaacggcatc tacgacggcc
    50341 tgctgaagtc cgccggcgcg acgctgttgc gcggctgggc gagcgtggcc gatgcgcaca
    50401 cggtggccgt ccgccctgtc gacggcgccg cgggctcatc cgtcaagcac tgcaccgccc
    50461 gccacatcct gctcgccacc ggtggccgcc cgcagaaggt cgatggcccg ggtgccgact
    50521 gggcgctgac gtcggacgac atgttcgacc tcgatccgtt cccgcgccgc ctggtcgtgg
    50581 tcggcggcgg ctacatcgcc tgcgagttcg cgtcgatttt caacggcctg ggtgcgcagg
    50641 tgaccctggt gcaccgcggg ccgcggctgc tgcgcggttt cgaccacgag gttgccgatt
    50701 tcgtcaccgc cgagattcgc aagaagggca tcaccgtgca cctgcaatcc acgatcgccg
    50761 acgtgcggcg ggccggtgcg gtgcagacgc tgcgcctgaa gcagcgtgct gctgcggccg
    50821 atgccggggc gcctgccgac atcgagctcg aagccgacgc cgtgctgcac gccaccgggc
    50881 gcatcgcgcg caccgacggt ctgggcctgg acgcgctcgg catcatgctg aacccgaacg
    50941 ggacggtgcc ggtcgacccg cacttccaga ccgtggtgcc gtcggtgcat gcgctcggcg
    51001 acctggtcgg gcacatggcg ttgacgccga tcgcgctggg cgaggcgatg ctgctggtcg
    51061 accacctgtt cggcgatggc gtgcggcggc cgatcgatta ctcgctggtc ccgacggcgg
    51121 tcttcacgca cccgaacatc ggaaccgtgg ggctgggtga agaagccgcg cgcgaacgcg
    51181 gcccggttcg catctaccgc agcgagttca agccgctgca gcacacgctg agcggcagca
    51241 ccgaacgcac gctgatgaag atcgtggtcg acgacgtgac cgaccgtgtg ctcgggatgc
    51301 acatggtcgg cgccgacgcc ggcgaggtga tccagggctt cgcttcggcg atgcaggccg
    51361 gccttaccaa ggcccagctc gatcgcacca tcggcatcca cccgacgggc gccgaggagt
    51421 tcgtgacgat gcgcgaggtg gcgcggcgtt gagctgtttc ggcactgcgt cggctgcact
    51481 gccgaaagcg ttcagccgcc cgcaggcggc tgattacgca gccccgccgc ggcgcagggc
    51541 ctgacttgcg cggcagcctt cagttgcttg ccaggctgat cgcgccgttg tcgactgtga
    51601 cgcgctgccc cacggtccag gcgggtgccg tgctgtggcg aatcgtgcgg cggctgccat
    51661 cgtcgaactt caccgtggtc tcccagccgc tggtgctgtt gttgttcttc tcgatggtgt
    51721 tgcctgcata ggcaccgcct gccacgcctg cgatggtcgc caccgcgcgg ccgctgcccg
    51781 agccgaactg gttgccgagc actcccccca gcacgccgcc gaccacggcg cccactccgc
    51841 ttgatgagcc ggtgcgcgtg acctgctgca ccgccatgac gacggccgga ccgccaccgc
    51901 cgtttgctgc ggtcccgatg tcgagcgtca agcgtgcggg ttcgctgcgc acgccctggg
    51961 catcgaccag cacgaactcc agcgtgctgc gcatcggctg cttggcggca tcgaacggaa
    52021 actgcaccgc acctgtcgcg ttgccaggct gcacggccgc cgtggcggaa acgtcgggcc
    52081 acggcacgct gctgtcgacg acgcgacgct cgatctgcac gatgtccgca gcaccctttt
    52141 ggtacttgat gtctacgttg aaggcgcgac ccacacccag tggccgtggc gccgtgacgc
    52201 tgctgatcac gatcggaggc aggatgttga aggtgcgctg gaacggtgcg ctgcgcttgc
    52261 cgtcggtcga caccagcacg tactcgaagg tcgcattcat cggctgagcc gttttgtagg
    52321 ccagtgtgcc ggcaccgacc tttgcttcgc cgctggtggc ttccgtcgcc gggtagctcg
    52381 cgacctggtt gttccagttg ccttcaccgc tcacgaaacg cacctcgaca tccttgacgg
    52441 tttcgcggcg agcgtggatc gccagcgtca ccggccgagc cttgccgtcg ccgaccatcg
    52501 gaccctgcat ctcgacggcg gtgatctcga acaccggccg cagaaaacca aaccacgaga
    52561 acgcaatcac gccgcacaac gccagcccgg ccactgcggc ctgcagcttg cggttgggag
    52621 gcacggggac cggcgccgat accgggtcgg ccgggccgcc gaaggtctgg ttggctgccg
    52681 agcggaacag ggcctgagcg ctgccggtgc cgccgtcggc gaaggccgcg ttcgagatca
    52741 gcgcttcaag ccccaagccc tctgcccagg tgtccactgc ccagcgcgag gcgttaggcg
    52801 ccatcccctt ctcgtcactc agccgcttgg ccagtcgcgg cgtcaagctc tggatcggct
    52861 cgccggaatg cacccgcatc agatcctgcg ggacccgttc ctccagcgcc tgcatcagca
    52921 ccgagacttc gcgcttggcc tgcgggcagt catcgtggag caggccgcgc aggcggccgg
    52981 attcgcccag cagtccaata ccgtaccggt cgagcagcga cttcagcttg gcgccgatgg
    53041 ttgggtcgta gaggctgtcg ctggggccgg aggcgggaaa ggcagtggtc gtcatggcag
    53101 ttctcctgtg cttgcggacg gatctcgcgg agtggcccgt gcgcgcttcg ttgatcggta
    53161 gggcctggag acgcaatcgg gcagcgaacc ttaccgaacg caaaaaatta tctgtctcgc
    53221 tcgcttcaca gcttgacctc gccgagcatg cgcccgaagt cgatgcgctg accctgactc
    53281 agcgagagcc gctcgcctgg ttccaccttc tcgacctgtg tcgacgggcc tgaccatacg
    53341 gaccaggtct ggcgcgacag gttcaccagc gccagagccg ggtcgacgac gatctgcgct
    53401 gcggagctcg ggtgccccga gttttgatcg tcgacgtggt gcgaaagcag ccgagccccg
    53461 ggatcgagca ccacgacgta caggggggcc tcctggctgc gggcctggag tcgcctcagg
    53521 ccgatgcgcg gcggcgaggt ggacaacgcg gcaccgcagt cccagcatgc gaattgatgc
    53581 tgctttgctg ccagccgctg ggggtcgtgg aaattctctg ccccgcaacc cctgcaggtc
    53641 agcaccgagt cgtggagttg ccgcagcgcc ttgcgccatt cggactcctg cacgcgaccg
    53701 tgcagcggat cgaacaatcc gtcggtgaac gcgcgggtga acaggtcgcg cagcgcctgc
    53761 ggatagatgg cccagtgccc cagcaccggg ccgtgccgat cgggcagcgg gcgattcgac
    53821 gcgtccgacg ggtcgaacac gaatcgggcg ccgctgccgt acagacggcg caagacggcc
    53881 tgatctgtca gattgtggtg ttcgagttca cagcgcccta tcaacggatg accgatgtgc
    53941 aggatgcgga acaacatcac cgccagcgaa tgcagatccg tcgcccggga cggcccgcat
    54001 tgccgcaaca ccacctcggg cgcctggaac tcccagaccc cacccatcac gctgggcgcg
    54061 ccgtcgatgt cgacgttatc gttgtcgcag atctcgatct ggccggtgct gcgatcgaaa
    54121 aacaggttgc cggcgttgag atcctgatac accagcccct tggtgtgcag cgccagcagc
    54181 gcttcggtca gacggcagca aacggtggcc agcacccgga acgtgggacg cacctctccg
    54241 gccagcacgc tctgaatctt gagatgtgat tccgcccgca atcgcatcag atagcccagg
    54301 ccgcttccgt cgggcaacgt caccagttcg aagggccaca ggaactccgt ggtcggagcg
    54361 ccccgctcga gcgctacctg cagccgttgg cgcagtccac ggtccacccg caacgcgagc
    54421 gggtggtacc acttcagcgc gtagggtcga ccgtcgaggg tgacccgata gacctctccc
    54481 tgcccgccct cgccaatcaa cacctcgacc cgacacgcca gctgcgagtc gacgcatctc
    54541 aacagctgac cggggcgcag ggcgggcatc gcaggtctcg cgggcttttt ttgtaggcag
    54601 gaaatgtggc gacccgtttt tcgtctccag accgggccgg gcatctggat caacaacgtt
    54661 cggatgcaat tgttaacaca aaccgagggc gttacgatgt atgcacgact ggatccgagc
    54721 aaggtggctt ccatggaaca gaaacggcct ggcggcgaca tgaaatcgcc ggcctcttcc
    54781 gaacccaccc ggtcgcagct gcttcctttg ggcggcgcat ggcaggaact cctcaccaag
    54841 ccctatttca tccccggtct tgtcgtcagc ctcgcgattg cgttgctgtt cagtttccgg
    54901 ggcgacaagt ccgccacgat caacgtgtcc tacggcggca tcccggtcgg cgttccggct
    54961 tacaccatcg tgctggcctt gttcatcgtg ttcggcggcg cttatggcgt ctaccgaatg
    55021 gcgggcaagt ccaaggcgtg gtggctgatg cccgtggttg ccatcttcac cggcctgctc
    55081 gcgttcagcc cgctgatggg tatgttgcag gatgtctacg gcgtcgtact ggggcgctcg
    55141 agcaaagccg acggctgggt gctgaagttc gttcagtcat tgttcaatgc cggcctgccc
    55201 gaagaaacgc tgaaggcgat accggtggcc atcggcgtct acatcgggat cagcctgctg
    55261 ggtcgtttca aggccggtca tccggcgcgc caactggcgg tgatcgaacc actggacggc
    55321 atcctgatag gcgtggcatc gggattcggg ttcgcgtttg cggaaaccgt tttccagtac
    55381 gtgcccggcg tcatcctgaa cagccaggag accgtccgtg ggttgcttta tgcgctgaag
    55441 accatcggct atccagtgcg cctgccgcag gacacctcgc aactgcagga catcgccgcc
    55501 ctgttctcgc tgctggccaa caagatcggt tttgagcgcg ccacgttcga actcgcgcag
    55561 atcctccaga accgtcaggg tgccgggctt gagctgatga ttccccggct gctttccgga
    55621 ctgttcgggc acgccgccta cgccggcatc ttcggatatt tcatcggtct ggccgcattg
    55681 aagccgggcg ctcgtgtcaa gacggtgctg attggcgtgg cggtggctgc gacgctgcat
    55741 gcgctgtgga attcgtcggc cggctcggtg atgatgttct tcgtcttgtc gatggcggcc
    55801 ttcgtctgtc tggcggtgac catcatcaag gcgcgcgcca tttctcccga gcgcgaccag
    55861 ctggcggcgt cgcagctgat cgatcgcttc gcacccggtg ggatgtcgac gatccgcccg
    55921 gtcgaaagag ccatgccggc tgcagcccct gacgcaaacg cggtcgccga gcgcaccact
    55981 gcgcacccag ccatggtgcc cgcgtccccg gcggcgcaga tcggctcgct gacctgggat
    56041 gacgactcca acctgcgggt gctcgaagtc ggtaccgccc gcatccctgc aacggcaggt
    56101 tcgcaccttt acgaacggca ggtgccgggt gcgcgttccg gccaagggga tgccgtggtt
    56161 gccgaggtca ccgcgcatcc gaacgacccg agcgtgctgg gtctgaagaa cctgagcgat
    56221 caatcctggt ccctggtcac ggtcgagggc gagcagcgcg agctggctcc cggtcgcagc
    56281 atccgcctgg tagccggact gcaggtcagg atcggcgacc tgtcggtgcg ggtacgctga
    56341 ggggttgcca tgaacgacac cgctgaactg aacgtgccgc gcccaggtgg tgcgatggca
    56401 cgccgcgagt tgcatttcat ctggctgctc gacacctccg gatcgatgaa ttccgatggc
    56461 aagatccagg cgttgaatgt cgcgatccgg gaggccatac cgcacctggt gtcggccgct
    56521 cgcgacaacc cgaatgtcga tgtgcgcgtg cgtgccatga ccttctccga cggcgcgcgc
    56581 tggcatatcg agacacccac gccggttcac gatctcaagt ggatcgatgt ggccgcgggc
    56641 ggtcacactg acaccggcac tgcgctgaag ttgctcgcgc aggcgttgaa agtgccgccg
    56701 atgcccgaga gggcggtatc gcccgtgctg gtgctggtga ccgacggaca ccacactgac
    56761 gatttcgaag ccggtctggc tgcgctgttg gcagagcgct ggggccgcga ggcagtgcgc
    56821 atggccatcg cgatcggacg cgatgttcat ctggcgtctt tgcagcagtt cattggcaac
    56881 gacgagatcc gcccggtcga ggccagccat ccggaggccc tggtgcagca gatccgctgg
    56941 gtgtcgcgca gtggcatcga cagcgtgtcg caggtcctcg atggtggccg gcggctgacg
    57001 gtcggggatc tggcgaacaa cagtgccgga acggtcgacg acgccgtcga atggtgatgt
    57061 cggcagaggc cgtgacgccc tggcaggtcg tcggccgcag cgtgcgtggc gcgacgcatc
    57121 tgcgcgacgg tttgcccaac caggacgcgg ttgcacatcg aacgatcgtg gagggcgggc
    57181 agcccgcgct ggcgatcgcc gcagtcgccg atgggcacgg cggtcgtcgc cacttccgca
    57241 gtgccgacgg cgcccgcatg gcggtcgagg tggcgttgca ggtgctgtgc tccatggcca
    57301 cgcacttcga gcagttgtcg cccgccgatc gcagcgaact ggccgcggca caggtgccga
    57361 tggccatcgt cagcgcctgg accgacgcgg ttcgcaggca cctggcaacg tgccccatct
    57421 ccgatgaaga atggctggcg ctcgatgccg cggaaggctc cgaaggatcg gcagccgtgc
    57481 gggccgatcc gctcctcgcc tatggtgcaa cgctgctcgc cgcgctgacg acgccgaaca
    57541 gcgtagccct gtggcagctc ggtgacggcg atgtgctggc ggtgtcgccc gacggcagcg
    57601 tcaaccggcc ggtggcaacc gacgagcgac tgctgggcaa cctgacgact tcgatctgcc
    57661 gtcctggtgc ggaagccgac ttccgctcgg tggtgctgtc ttcgcattct gcgttgccgg
    57721 ccctgctgat gctgagcacc gacggctacg cgaactcgtt tcgcaccgat gctgatttcc
    57781 tgaaggtcgg cgctgacctg ctcgaactga tctcgaagca cgggctgtcg gcaatcgatg
    57841 cccggctggg tggaatcctc gaagacgcgt ccgcgaacgg cagcggcgac gacatcacgg
    57901 cggcactcct gtaccggtcc gggtcagaac catcagtgct ctcgacgtcc ggaggcggct
    57961 ggccacctgc ggaactcgcc gtggcgtctg ccgccccctc ggacggcggt caggcgctgg
    58021 ccgcagccca gcggcgcatc gcccgcttgc gatcggtgat cgtcgtgctg ctggttgtgc
    58081 tgctcgccgg ggcgctgtgg tggtcgcgcg atcggtccag gccgaaatcg gcggacttgc
    58141 ccgcgctcgg ggccccgatc ggatgggacg caccggccag cggccccaag ccgtcggtcg
    58201 aagctgccga gccagcgcct gtcgtgagtc cccagggcgc ggccaagccg ccgctcgctg
    58261 ccaaaccgaa gccggcagcg tcggccgcgt cgcgaccgaa gccagcgact gcgccagccg
    58321 cgacttcgaa gccgacgcct gaattgccgc cttactgacc gcacgcgggt ccccggcgga
    58381 aggcccgccg gctgcctgat gagtgatgtc agcgcttcat cgaatgcggc gcgcgcaact
    58441 catccagatg gcgctgtgcc tcgacactgc cggcggcagc cgcctgctgc cagtagcgct
    58501 cggcggcgac gatgtcgacg gctacgccca gaccctgggc atacagggtg cccagcgcca
    58561 gcgccgcggt gacattgccg cgctcggcgg cgtccttcaa cagccgaacc agcttggcat
    58621 ggtcgccccg tcgatttgcc tcgacggcct ctgccgacac ctcggccagg cgcgtcatca
    58681 cgtcgctccc gtccggaacc ggctgggcgt gggtggtgtg atcgtcggcg ggcaatcgag
    58741 ggtcctgcat caccacggtg atcgccacgg cggcgactac cgacgcgagt gcgatctgcg
    58801 gcagcagtcg cggaccggcc ttcagccagg cgaaccagcc ggaacttgcc gtgcgcgact
    58861 cgacgcgctg tctcagccgg ggtgcgtggg ccgcaagatc ttgaagctcg gtggcgcacc
    58921 gcgcgcacca cgccgtgtga gtctcgacgc gctcgcgcag cgccggttcg gcacgcccgt
    58981 ccacatgcgc ttcgagttgc tcgtaactca ggtgcgcgcc gctgctgacg acatggcgaa
    59041 tcaggcgtgc ggcttccttc atcgacggtt cggtgtcagg tgtcatggtg tgctcctgaa
    59101 ccacaggtga cgtgaaatga ccgccgacat tgcgagtgcc ttgtgcgatg gcgaagaatt
    59161 cctcatcaag gggccttcag gcgattcgac gtgtgggcca tgagccgtgc cagttcgcgc
    59221 agcgcgagca tccggcggtt gatgacctgc tgcgcgtcaa gccgcagcag cgcaccgatc
    59281 aaggcatcca tcaacggcag gtggtcccac aggatctcga agcggtggac gtctgcactg
    59341 cagcgttcga ccgcagcgcg tgcggctgaa tccaggtcga gctgagcgaa ggcgatgcgg
    59401 tactgtcccg catccagtcc tacggcagcg gaaatctcgc agatcgaggc gatgtcgtgc
    59461 atgacgaaca cctcgatgtc gccccgcgac ttgccgacgc ctggcaggtt cagcaggtaa
    59521 gcggtgcggt agtctggctt cagcacgagg accgcgcccc acaagtgcct cagcatgctg
    59581 cggacctcgg ccgcttgctg tggtgacaag gctgtcgggt ctggcaaatc cggcgttgtc
    59641 tcgtcgtcca gcatggcgtc gagggaatcg gtgcggtctt cctggacctt cagcaaggcg
    59701 acggtcaccg acaccagatc gtcgagcgcg attggtgcct gcaggcgctc gaacaacgct
    59761 gccagcaggt ggtcccaatc ctcggcggca aagcgctcca tgtcctttcg cggcacgctg
    59821 cccgcggcca ggcgctcgcg cccgtcgcgc agcgccgcga ctcgggcatc accaactggc
    59881 accctgctgc tcagttgcca gccgtgcagt ccgatcagca actcgccatc gctggcattc
    59941 cagctggcgt agcgcgcctg gtgcgacgtg aagtaacgca agcggttctt caggctggcg
    60001 cgcttcggat acttatcccg caggtggtcg gaccaggcgt tgtaggtcac cctggcagcg
    60061 tacgagccga gatcgtccca ggcggcgccc ggggtggtgc tctgccgctc ccacaggcgc
    60121 aggatcacgt cgtggaccag ttccaccgct tcctgattct ccgggcgagg atcgtcgtcg
    60181 cgcagcgaca ggcccgactt gcgacgcaag atggcccgca cggtctcgcc gacctggagg
    60241 agcaaggaac tggtttcttc cgcgttgccc atgaacttga cggcggtggc gctggctgaa
    60301 tgtatcagcg cccgggcagc gtcgcggcct tcgcgcgccc gcgatcaggc gcaccgcaac
    60361 caccgtgtgc cgcagtcagc gcagcgtgta gccgcgcccg tccatgcagg cctcggtggc
    60421 acggttgaac acgctggcgt cgctcatggc actcggctgc gtcgtggccc agcgattgca
    60481 ctcctggcgg tcggcttccg tctgctgagc actctggccg ttgcgagggt agatgatcgg
    60541 atcggggcgt gcgacgacca ccggttcgcg cgcgggcacc tcgcggtaga cggtgcgcgg
    60601 cgcttcacga tagaccacgg gtggcacctc gcgatagacc accggcggtg ggtcgatcag
    60661 cacgtagccg ggttcggcgt agcctgcgta caccggcggg ccgtatgaat acgagtacct
    60721 cgacgcgccc agccagccca gcccgagccc gaggccgaac cccaggccga caccacccca
    60781 gcccggcccg tagtagcggc cgtggccacc gtgtccgcct ccgtgacgtc ccccggcctg
    60841 ggcatcggcc gcaggcagtc cgacggccag gatcaccgca gcgagggcgg tggtgcatgt
    60901 gcgcgtgaag atgttcatcg tgggctcctt tcgaaatggc cgacccggtc tgattttcag
    60961 accggcgatg cgcccgctgg ttccgaactt tgcaattctt gactcgattc gtccgatcgg
    61021 cctgctacgc tgacgcatga cttctcgcat cgcgttgcgg ggcgacctgc tcgatttcac
    61081 cgccgacccg ggctttgcac cgacttcgga gccaccgggc ctgcgctggc gccccgacca
    61141 ctggttgatg atcgaggacg gccgaatcat caacgcgctc cccgcgggat cggtggacac
    61201 gatggcggat ggcggtcctg acgccagctg gcagcgcgtc gatcaccgcg gccggctgat
    61261 cctgccgggg ttcatcgaca cccatgtgca cagccctcag ctcgacgtga tcgcgagcca
    61321 cggcggcgcg ctgctcgact ggctggagcg ctacaccttt cctgccgaac agcgccatgc
    61381 cgaccccgcc gtggccgaag ccggtgcccg ccgattcgtc gacgcgctgc tggcccacgg
    61441 cacgacggcg gcggtcgtct tcccgacggt gcacaaggtc tcggccgaag cgctgttcga
    61501 tgccgcgcag cggcgtggca tgcggctggt ctgcggcaag gtgctgatgg actgccgtgc
    61561 gcccgaaggc ctgcgcgacg acgtcgccca ggccgagcgc gactgcaccg aactgatcga
    61621 gcgctggcac ggccacgggc ggctggccta cgcggtcacg gtgcgctttg cgggtacgag
    61681 cacgcgcgag caactgacca tggccggacg gttgtgcgtt gcgcacgaag gggtgtacat
    61741 gcagacccac gtggccgaga acaccgacga ggtgcgctgg attgccggcc tgttccccga
    61801 ggcgcgcagc tatctggatg tctacgagcg gcacggcctg ctccacgagc gctcggtgct
    61861 ggcgcatggc atctggctgg acgacaccga ccgcgcgctg ctgcgcgagc gcggcgcgca
    61921 gattgccttc tgcccgtcgt ccaacctctt cctgggcagc gggctcttcg actggcaggc
    61981 tgcggccgat gccggggtcc aggtgtcgat ggccagcgac gtcggcgcgg gcacgagtct
    62041 ctcgatgctg cgcacgctgg ccgatggcta caaggtccag tcgctgcggg gcttgcgccc
    62101 gagcgcctgg aagctgctgc acgccgcgac gctgggcgcg gcgcaagcgc tggatctcga
    62161 cgcggagatc ggcagcctgc agacgggctg cgtcgccgat ctgaccgtgt gggactgggc
    62221 ggtcggtccg atcgccaccc accgcgacgg cctggcgcgc gatctgcatg agcgcgcctt
    62281 cgcctggatg acactgggcg acgaacgcaa cctggtggcg acctacgtcg cagggaccag
    62341 cgtccaccag cgagcctgat gtcactgaaa ggaagaacat gagcccgaac ctgcccgccg
    62401 gcatcgatgc cattccatcc gggcggctgc cagcgctgct gcgcgagatg ccgaaggccg
    62461 aactgcacat ccacatcgaa ggctcgcttg aacccgagct gatcttcgcg ctcgcgcagc
    62521 gcaacggtgt gccgctggcc tacgccagcg tcgacgcgct gcgctcggca tacgccttca
    62581 gcgacctgca gagcttcctc gacatctact acgccggagc atcggtgctg ctccacgagg
    62641 ccgacttcta cgagatggcg atggcgtact tccgccgcgc tgcggccgat cacgtggtgc
    62701 acgccgaact gttcttcgac ccgcagacgc acaccgaacg cggcgtgccg atcgaggtgg
    62761 tcatcaacgg actggttcgc gcgtgcaagg cggccgaggc cgagttcggc atcacgtcgc
    62821 tgctgattct ttgcttcctt cgacacctga gcgaggaagc cgccttcgag acgctggagc
    62881 aggcattgcc gtcgatccgg gagcacggcg accggctcgt cggcatcggc ctcgacagca
    62941 gcgagcgcgg tcacccgccg gagaagttct cgcgcgtcta cgcccgttgc cgcgaactcg
    63001 gcctgaagct ggtggcccat gccggagagg agggaccgcc ggcctacatc tggagcgcgc
    63061 tcgacacgct gaaagtcgcg cgcatcgacc acggcgtgcg ctgcgtcgag gacccggcgc
    63121 tggtggcgcg gctggctgcc gagcgcgtgc ccctgaccgt gtgcccgctg tcgaacgtca
    63181 agctgcgcgt attcgacacg ctggcacagc acaacctggc aaggctgctg gacctgggcc
    63241 tgtgcgcgac cgtcaactcc gacgacccgg cgtacttcgg cggctacatg aaccagaacc
    63301 tggtcgagac cttcgcggcg ctgccggaac tgaccgctgc tcacgcctac acgctggcac
    63361 gaaacagctt cgatgccagt tttgccagcg cgtcacagaa ggcccgctgg gtgcaggcgc
    63421 tggacgcgtg cttcgcgcag gggtgagtcg cgcttcgtcg cgctagggct ggtcggtgac
    63481 cttcgacgat tgcagtccct ggtcgccgaa ctgcagggcg caggtgcggc ggccggacat
    63541 ccgggggtcc tgcacaagca cgcgcccatc ggcgagcgtc gacaaggtgt agccccgcgt
    63601 ggcggcctga gcctgcacgt cggcgagcgg cgttcccgcg gccagtgctt cacagaatcc
    63661 ctgcatgcgc tgtgccgtcg ccatgaaggg ccagaaggcg aaggcaacgc cggccagcat
    63721 catcacggcc atcccgacga atatccagcg gcgcatcgat gtgcgtgtct cttcggggag
    63781 tgcaaacaat ccggatcgtg tcgccatgtc tgtcaagtcc tcggtgtcgt gcggggttgc
    63841 ggtgagttca ttcgcgccgt gtcgccgtgc ctgcttcgaa ccagcgacgc aacgtgtctc
    63901 gttcctggtc ggtcagctgc gttgcgttgt tcatcggcat ctgcttcagc accacggcct
    63961 gctggtagat cgccagcgcg tgctgctcca cctgccgatc cgtgtgcagc gccacgccct
    64021 tgttcgccag tgtggcgttg tggcacatca cgcagcgctg gtcgatgatg gtttgcacct
    64081 gcgccaacgt gaccggggag ccggccggcg acgatgccgg gggtgacggc ggtttcagcc
    64141 acaccgccgt gccgatcaac agcagcaccc cgaggatcgc gtgctcccat ggcacgcgtc
    64201 ggccaagcgc cagcgcgcgt cgccgggcga cgaacgagtg gcgtatcaag gcgccggccg
    64261 acatcagcaa catcagcacg gccgcggagt gtggcgcgct gtaaaggaac ggatagtggt
    64321 tgccgagcat ggccaccagc acgggcaggc cgaagtaggt gttgtgcacg ctgcgctgct
    64381 tggcgcgctg accgtgaatc gggtcgacgg ccgtgccggc ctgcatctgc gcgatcaccc
    64441 ggcgctggcc ggggatgatc cagatgagca cattggcgct catcgcggtc gccatcgcgg
    64501 cgccgacgat caggaaggct gcacgacccg cgaaaagtgc gcacacggcc acggaggtga
    64561 tgccgaccag caaggccaca ccgaatccgg cgatgcggtc gccgttcgat cccgtgccca
    64621 gcgtgcggca cagcatgtcg tatgcgaacc agaacagcgc cagcaccgcc agcgccgacg
    64681 ccactgcggc agcagccgac cattcgtgca ccttcgggtc gatcaggtag gtgcccgcct
    64741 gcagcaggta cagcagcgtg aacagcccga agccggtgag ccaggtcgag tagctctccc
    64801 agtagaacca gtgcagcttg tcgggcagcg cgggtggtgc gacagcgtac ttctgcgggt
    64861 ggtagaaccc gccgccgtgc accgcccaca gctcgccgct cacgccgcgc gacttcgagg
    64921 cctcgtctgc cggcggcgtc aggctgttgt ccaggaaaac gaagtagaac gacgagccga
    64981 tccatgcgat cgcgaccacc acatgcgccc agcgcaacag caggttcagc cagtcggtca
    65041 ggtacgggtc catcccgcgg tcacggcctg ctcaagggat cagcttccgc ggtaggtcga
    65101 ataggcccac gggctcgcca gcaggggcac gtgatagtgc ccgccagcct cgccgatccc
    65161 gaagtcgatg ccgacttcgt cgagaaacgg cggatcggga agtaccgccc cgcgaagccg
    65221 gaaataggct gccacctgga agaccagtcg ataacgccct gctgccaggg ccgatgcggc
    65281 ctgggccaga tcgggcgcgc ggccgtcgtc gttcagcacg ctccgatgca gcagactcac
    65341 cgattcgctg ccgacgctgt agagcgccac ggccatgccc gcagcgggac agccgtgcat
    65401 cgtgtccagc gcgtgggtgc tgagggttgc catgggtgca gcgatgttcc agcggccggg
    65461 tcggccgtgc gggctcgagt tttgcacacc gatcggtgcg gcgagcaggc gatgatccgc
    65521 ctgtgccgac gaaccctgga actggcccga catgaacgct caccacgacc gtgcgctgaa
    65581 cctcgatcgc atcaacaccg cgccgctggc cgacgtgctc gccttgctcg acggcctcta
    65641 cgagcattcg tcgtgggtgg tcgaggcggc gctggctggc cggccgttcg cgaccctggc
    65701 cgacctgaag cacgcgatgg ccgaggtggt gcgccatgca ggccatgaaa ggcagctggt
    65761 gctgatccgc gcgcaccccg aactcgcggg cagcgcgctg acggccggca cactgacacg
    65821 cgattcgtcg cacgagcagt cgctgtcggg actggctcac ggatcgcccg atgaggtgca
    65881 cgcactgcac gagctcaaca acgagtacac cgcgcgcttc ggctggccgt tcgtgctggc
    65941 ggtgcggggc cccacggggc gagggctgac gagagcggag atcatcgcgg cactgcggca
    66001 ccggctggtg gccacgccgg aagtcgagat ggacgagtgc ctgaggcaga tcgaccgcat
    66061 cgcggagatc cggctacatg agcggatggg gctcggtttg gaggcgggat gaacggacag
    66121 ccgatccgat tcttccaccg cggtgcgatc accgaagtca gcggggtgca tccgacgttg
    66181 tcggtgctcg actggctgcg tgatcacgcg cgctgcaccg gcaccaagga aggctgcaac
    66241 gaaggcgatt gcggtgcctg caccgtcgtc atcggcgaac tgccggaacg caccgaccgc
    66301 ccggccgagc atgtgcgggg cctgagcctg cgcagcgtca acgcctgcct gcagttcctg
    66361 cctacgctga acggcaaggc gctcttcacg gtcgaggacc tgaagaccgt ggctggcggt
    66421 gcgctccatc cggtgcagca ggcgatggtc gagtgccacg gttcgcaatg cggcttctgc
    66481 acgcccggca tcgtgatgtc gctgttcgcg gactacgagc agcaccggac cgccggccgt
    66541 cggccggacc gtcagcaggt cgccgatgcc ctgtcgggca acctgtgccg ctgcaccggc
    66601 taccggccga tcctcgatgc cggggagcgc ctgttcgatc ttccctcggt gcaactcgat
    66661 accgcgccgg tgattgcggc actgcgatct ctgcagcaga ccgggacttt cagctatggc
    66721 gcggcgaatc gccatggccc tgcggaaggt ggcggatcgc gcatcgatca cttccatgcg
    66781 ccgacctcgc tggccgacct ggcggccctg cggctggcga acccggcagc gaggctgcta
    66841 gcgggctcga ccgacatcgg actgtgggtc acgaagcagt tccgcgacct gggtgacatc
    66901 gtctacctcg gcaacgtcac cgagctgaag caggtcgaga cccgcgacgg cgaattgcac
    66961 atcggcgcag gcgtgtcgct cgaaacggcc tggcgagcgc tggccgagcg ctggcccggc
    67021 gtggccgaag tgggcttgcg tttcgccggc ccgccggtgc gtcatgccgg gacgctgggc
    67081 ggcaacgtgg cgaacggctc ggcgatcgga gacgcagcgc cggtgctgat ggcgctggat
    67141 gcgcagatcg aactgcggcg cggcgacacc gtccgccgat tgccgctgca gttcttctat
    67201 gtcgactaca tgcgaaacct gatgcagcgt ggtgaattcg tccagggcct cgcggttccg
    67261 ctggccacct cacggcgcag cgtgcgtgcc tacaagatca gcaagcgcca cgactgcgac
    67321 atctcggcac tgtgcgcggc ctttgccatc gacatcgaag gcggcacggt gcgttcggtg
    67381 cggctggcct atggcggcat ggcggctgtg gtgaagcgcg cggcagcggc cgaagcggcg
    67441 ctggtcggag gggcctggag cgccgaatcg gtgaaagccg cgcagcgggc actggtgcag
    67501 gacttccagc cgctgtccga cctgcgcgcc agcgcggatt accgcctgca ggtcgcgggg
    67561 aacctgctgc agcgcttctg gctcgagacg cgagccgtcg acccgttgcc ggcgcaggcc
    67621 acgcgcgtct ggccgatccg gtcgctggac agcgcgagcg tggacacgcc accatgaacg
    67681 actcgctcgc cgctgcggga cccggctcac ctgccgaggc cgtcgaaccg gccgggctgg
    67741 tttccgcggg ctgcgcaggc gtgggcgttg cccgcccaca cgaatctgcg cgcctgcacg
    67801 tggccggcga ggcgccgtac accgacgacc tgcccgagct cgttggcacc gtgcacatcg
    67861 ccctgggcct gtcgccggtg gcgagtggcc ggttgatcgg cctttcgctc gatcgcgtca
    67921 gggccatgcc gggtgtcgtg gcggtgttca ccggcgccga cttccctggc cccaacgatt
    67981 gcggtgcgat cgtccacgac gagcccatcc tggcgaccat ctcgttcgac ggaccgaact
    68041 cctgtgttgg cgagctgcgt tacctcgggc agccggtgtt tgcggtggtt gccgacaccc
    68101 gcgatgcggc ccgccgtgcc gctgcgcagg ccccgcacgt gatgaccatc gtgccgctgc
    68161 ctgcggtgct gacgatgcag gaggcgcacg cgcggtccga ctacgtgctg cctcccctgc
    68221 atctgaaccg cagcatcagc ggccggggtg cgcacgccgc gatcgaaggc gcaccgcatc
    68281 gcctgagcgg cgagttcgag atcggcgggc aggagcattt ctacctcgaa ggccagatca
    68341 gctgtgcgca gcccacggag gacgacgggc tgctggtgcg ttgctcgacc cagcacccga
    68401 gcgagatgca gcatgtcgtg gcaactgcgc tgaagctgcc tgcgcatcgg gtgcgcgtcg
    68461 aatgccgtcg catgggcggc ggattcggag gcaaggaatc gcagtcggcc ccgttcgcct
    68521 gcattgccgc gctggtcgcg cgccggctcg atcgaccggc caagctgcgc ctggatcggg
    68581 atgacgactt catggtcacg ggccgtcgcc acggcttcca catcgactat gacgtcggtc
    68641 acgacggcga gggccgggtg cttggcgccg aggtcacgct gctcgcgaat gccgggcact
    68701 cggccgacct gtcggccccg gtgatgtcgc gcgcgctgtg ccatgtcgac aacgcctact
    68761 ggctgcccga ggttgcgttg cacggcttct gctgtcgcac gaacacgcag agcaacacgg
    68821 cgttccgcgg tttcggcggg ccgcaggggg cgatcgcgat cgagttcatc ctcgacggca
    68881 tcgcccgacg gctgggcctc gatccgctcg atgtgcgccg cgccaacttc tacggcgtgg
    68941 acgagcgcaa caccgcgcct tacggccagc tcatcgcgga caacatcctc cagccgctgg
    69001 tcgatcgcct ggatcgcacc agcgattacc gcacgcgccg tgccgccatc gcgctgttca
    69061 acgcgggcag cccggtgctg aagcggggaa tggcgctcac gccagtgaag ttcggcatcg
    69121 cgttcaacct gtcgcacctc aaccaggccg gtgccctggt gcatgtctac accgatggca
    69181 gcgtgctggt gaaccacggc ggcaccgaga tggggcaggg gctgaacacc aaggtcgcgc
    69241 aggtggttgc gcacgaactg ggcatcgcct tcgagcgcgt gcgcgtgacc gcgaccgaca
    69301 ccgacaaggt ggtcaacacg tcagcgacgg ccgcatccac cggcaccgac ctcaacggca
    69361 aggcggcaca gaacgcggcg cgccggattc gcgatcgcct ggcagccttc gcgtcgggct
    69421 tgcacggggg cgaggcggct ggggtgatct tcgccaacga tgcggtccgc gtcggtgatc
    69481 ggaccctgtc gttcaccgac ctggtcgcgc aggcgtacgt gggccgcgtg cagctgtggt
    69541 ccgatggtta ctacgcgaca cctgggctgc actgggaccg cgagacgatg caggggcatc
    69601 cgttccagta ccacgcctgg ggtgcggcgg tcagcgaagt cgtcatcgac acgctgaccg
    69661 gtgaatggaa gctgctgcgc gccgacctgc tccacgatgt cggcacctcg ttgaatcccg
//
LOCUS       DAOORV010000003        67747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_431_length_67747_cov_8.466613, whole genome shotgun sequence.
ACCESSION   DAOORV010000003 DAOORV010000000
VERSION     DAOORV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 67747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_67747_cov_8.466613"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="leuS"
                     /locus_tag="PKV56_00710"
     CDS             complement(<1..1798)
                     /gene="leuS"
                     /locus_tag="PKV56_00710"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOX66353.1"
                     /translation="MNPEFKPTEVERAAQAAWSARDAYRVTEDTSRPKFYACSMLPYP
                     SGKLHMGHVRNYTINDMLTRQLRMKGFNVLMPMGWDAFGLPAENAAMKNKVPPAKWTY
                     DNIAHMKGQMQAMGLAIDWSREVTTCSPDYYRWNQWLFLKMLEAGIAERRTQVVNWDP
                     VDQTVLANEQVIDGKGWRSGALVEKREIPGYYLNITKYADELLDQVQHHLPGWPERVR
                     LMQENWIGKSEGVRFAFSHDISDHSGKTIQDGRLYVFTTRADTVMGVTFCAVAPEHPL
                     AVHAAASNPAVAEFIAECQAGGTTEAELATQEKRGVRTGLTVQHPLTHQPVEVWVGNY
                     VLMSYGDGAVMGVPAHDERDFAFARKYGIDILQVVHVDGEPHFDYERWQDWYADKQRG
                     VTINSDYFSGLSCAAAVDAVAKALETHGLGAKKTTWRLRDWGISRQRYWGTPIPIIHC
                     ETHGAVPVPECDLPVVLPEDCIPDGSGNPLHKHAGFHAGVTCPVCGAPARRETDTMDT
                     FVDSSWYFMRYCDPKNTDAMVGDGAAYWMPMDQYIGGIEHAILHLLYARFWTKVMRDL
                     KLVSVDEPFTKLLTQGMVLNHIYSRKTEQGGIE"
     regulatory      1933..2044
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2117..4015
                     /gene="thiC"
                     /locus_tag="PKV56_00715"
     CDS             2117..4015
                     /gene="thiC"
                     /locus_tag="PKV56_00715"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOX66354.1"
                     /translation="MAKNLPHVDTDALSQRITRTPFPGSRKVYLDGSSPDIRVPFREI
                     TLGDTLVDDGAGGSRREPNPPLRVYDASGPYTDPQAAIDITCGLPPLRAAWIADRADT
                     VALPGISSAYGRQRLHDPKLTALRMAQAPVPRCAKPGANVSQMHYARRGIVTPEMHYI
                     AVRENLVKAQLRERLATERVPKRGHSFGANLQDITPEFVRDEVARGRAVIPSNINHPE
                     SEPMIIGRNFLVKVNANIGNSAVTSSIEEEVDKLVWSIRWGADTVMDLSTGENIHETR
                     EWILRNSPVPIGTVPIYQALEKVHGKAEDLTWELFRDTLIEQAEQGVDYFTIHAGVRL
                     AYIPLTAERLTGIVSRGGSIMAKWCLSHHRESFLYEHFDEICEIMKAYDVCFSLGDGL
                     RPGSIADANDEAQFAELHTLGELTQVAWKHDVQVMIEGPGHVPLQLIKENVDKQLEAC
                     YEAPFYTLGPLITDISPGYDHISSAMGAANIGWYGTAMLCYVTPKEHLGLPNRDDVKQ
                     GLIAYKIAAHAGDLAKGFAGAQMWDNAVSKARFEFRWEDQFRLAIDPDTAMAYHDETL
                     PKENAKVAHFCSMCGPKFCSMKISQEVRDFARLQNVEASVSAVIPIQTALEEKAMQFR
                     EGGGEIYA"
     gene            4358..5008
                     /locus_tag="PKV56_00720"
     CDS             4358..5008
                     /locus_tag="PKV56_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66355.1"
                     /translation="MDVDKMHQSPEEAPTSASGSGSTEPLSASALARRRALFKGIGKG
                     GAVLAASVPIQTLAGQTLLTAGGQHICSVSGMQSGVHSATTNVAYCGGYSPGWWGQST
                     DQGGLIPKRWPPTLEHGWTYKTLCKDVFSGSSVRNDDGSFPSLFQVMSPKDKSGDFSN
                     SDEFHWVCAWLNAITHSFNFPYNGDQVIAFWNAGATSDTYKDALSFFKTYMEQHTS"
     gene            5038..5316
                     /locus_tag="PKV56_00725"
     CDS             5038..5316
                     /locus_tag="PKV56_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HOX66356.1"
                     /translation="MRWRTRSLASLHWRCWESQWVVFNVGSGQTHLLDAVTAAVLSTI
                     ESSPGNADDLSALELQVATLGERILPSDTLKSVLEQLVGAGLIEAATS"
     gene            5370..6230
                     /locus_tag="PKV56_00730"
     CDS             5370..6230
                     /locus_tag="PKV56_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HOX66357.1"
                     /translation="MLLRTGPFNFRIVSAIESVAAGLRLLYADYPLAADDDFVDFTVT
                     LQCSGGLRRWWRRQVILGYDGANPFVPLPLDHAFPLLEWAMNWCISTQAHHVLTLHAA
                     VLERGGRAMILPAPPGSGKSTLCAGLTSRDWRLLSDELTLLSLSDGSVVPLGRPISLK
                     NESLAVIRQFAPDVVLNDVTHDTSKGSVSHMKVPSDQVRRLDEGARPRWVVFPKYVPD
                     APPTLRPRSKAESMLELGRNAFNYTLLGLTGFEVLADVVDGCDCYDFSYSRLEDAVAV
                     FDRLVVETPP"
     gene            6230..7336
                     /locus_tag="PKV56_00735"
     CDS             6230..7336
                     /locus_tag="PKV56_00735"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX66358.1"
                     /translation="MSDLLSPVWAQPELRPEFSLAQWEAVLGQSRQAGLMGRLARHHV
                     DHGWMTEVPAKPRLYLEGALRQVDRLHHELRWEIDCIHRALAPVGVPFVLLKGAAYFA
                     ADLPPKRGRLFGDIDIMVRREHLQQVESALFRAGWISDERDAYNQRYYREWMHEIPPM
                     RHVQRGTVIDVHHTITPPTSQMCVEGEALLEHIVAVDALQDVYVLAPVDMVLHSAVHL
                     FQEGEFERGLRDLLDLNDLVHHFESHDPDFWRQLISRAHALKLQTPLHHALFHLKRLF
                     GTHVPPAFQGAVELLRPPWWQRRLMAMLLEAALRPVHPSCRQTGEGLARWLLYVRSHA
                     LRMPMTLLVPHLVRKAWMQRFPTKKPEDHPAAEA"
     gene            complement(7357..7791)
                     /locus_tag="PKV56_00740"
     CDS             complement(7357..7791)
                     /locus_tag="PKV56_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX66359.1"
                     /translation="MAFGRLERTTGPQPMSDINMTPLIDVMLVLLVIFMITAPLMSSS
                     LKLDLPKTEAAQPSESPEFITVSIDSGGQLFLADSEITAAALAQRVAETARLNPQTEV
                     QLRADQAVPYGRVAELIGIVQQGGLTRIGFVAIAPVADKPKQ"
     gene            complement(7801..8475)
                     /locus_tag="PKV56_00745"
     CDS             complement(7801..8475)
                     /locus_tag="PKV56_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX66360.1"
                     /translation="MNGFQQFWQQGDAITRSVAALLLVMSISAWVLIFWKGWLLRRVR
                     SDIARAVPAFWAAADLEAARDQLAAFDRERVLTPLLTAATAQPNSQTLEAHGQRASQL
                     TRRLRDALHAVLTQLQFGQVLLASIGSTAPFIGLFGTVWGIYHALVSISSAGTITIER
                     ISGPVGEALVMTAAGLAVAIPAVLAYNIFGKLVGTCEAELEGFAHDLREMQVDRTSDP
                     STPQGS"
     gene            complement(8510..9337)
                     /gene="dapB"
                     /locus_tag="PKV56_00750"
     CDS             complement(8510..9337)
                     /gene="dapB"
                     /locus_tag="PKV56_00750"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOX66361.1"
                     /translation="MSFPLGAAEPLRIAIAGASGRMGRMLIEAVIAADDLSLSGALDM
                     PGSPALGQDATAFAGHNSGVIITSDLHAGLARSQVLIDFTRPEGTLAHLAICRELGTK
                     VVIGTTGFADAQKAEIADHARHIAITLAPNMSVGVNVVMKLLDTAARMLSEGYDIEII
                     EAHHRHKVDAPSGTALKMGEVVATALGRNLKDCAVYGREGVTGERDPSTIGFATIRGG
                     DVVGDHTVLFAGIGERIEITHKSSSRATYAQGSLRAARFLASRESGLFDMNDVLGLS"
     gene            complement(9337..9882)
                     /locus_tag="PKV56_00755"
     CDS             complement(9337..9882)
                     /locus_tag="PKV56_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOX66362.1"
                     /translation="MSLLPLARSPVHIVSLALAAACSSGCSSLDPSGTILSVVTPYRV
                     DIVQGNVVTKEQVARVQIGMSRAQVRDILGSPLLADVFHADRWDYVFTIRRQGTEPQR
                     RSIVALFEGERLKSLDTGGDLPAEIAFVASIDSNKSSGKAPSLALTPEQIQSLKPPPK
                     AAQVEAPVVAPDRVYPPLESN"
     gene            9980..10408
                     /gene="fur"
                     /locus_tag="PKV56_00760"
     CDS             9980..10408
                     /gene="fur"
                     /locus_tag="PKV56_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HOX66363.1"
                     /translation="MTNADELKNSGLKATLPRIKILEVFQQTRQRHMTAEDVYKVLLA
                     EGSDVGLATVYRVLMQFEQAGILSRNHFESGKSVFELNEGNHHDHLVCMDCGRVEEFF
                     DAEIEKRQRSVAQTRGFELQEHALSLYVVCTKKACPHRAS"
     gene            complement(10450..11403)
                     /gene="hprK"
                     /locus_tag="PKV56_00765"
     CDS             complement(10450..11403)
                     /gene="hprK"
                     /locus_tag="PKV56_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOX66364.1"
                     /translation="MKPISISAEALFEHHRVALRWEWIAGHAHPERRFDEAAVRNAQS
                     AADLVGYLNYIHPYRVQLVGRREVAYLLDASAEDQERRIQRIVTLEPPVVIVADDQVP
                     PDRLVAMCERASIPLFVTAESAGQVIDMVRGYLSQLFAERTTRHGVFMDILGLGVLLT
                     GESGLGKSELGLELISRGHGLVADDAVDIYRMSQTALEGRCPELLINLLEVRGIGLLD
                     IKAIFGETAVRRKMRVKLIVHLVRKETMEREFERLPYEPLYENILDVPVRKAVIAVDA
                     GRNLAVLVEAAVRNTILQLRGIDTYKEFIERHQRALERGGS"
     gene            complement(11465..11935)
                     /locus_tag="PKV56_00770"
     CDS             complement(11465..11935)
                     /locus_tag="PKV56_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOX66365.1"
                     /translation="MNRLAAILPVSNVLVDVDATSKKRVFEHAGLLFENQHAIARATV
                     TDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAVARVQQPIPFDAPDDEPVTLLIF
                     LLVPEAATQRHLEILSEIAEMLSDRELRERLKTEVDAAKVHELIANWEPLKSVA"
     gene            complement(12176..12517)
                     /gene="raiA"
                     /locus_tag="PKV56_00775"
     CDS             complement(12176..12517)
                     /gene="raiA"
                     /locus_tag="PKV56_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOX66366.1"
                     /translation="MNLTISGHHLEITPSLREYVLTKLDRVTRHFDQVVDINVLLSVE
                     KMTEKERRQKAEVTLHVKGKDIFIEHAAEDMYAAVDQLMDKLDRQVCRHKGRLQDHHH
                     VAAKRLDARAA"
     gene            12897..13856
                     /gene="corA"
                     /locus_tag="PKV56_00780"
     CDS             12897..13856
                     /gene="corA"
                     /locus_tag="PKV56_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOX66367.1"
                     /translation="MLNVFSLANGRLFQEEIESRAALSNVQPVWVDLDAPTAEEKGWI
                     AQHFGVTIPDDAVDDDLEESARFYEEDNGELHIRSDFLIDDDETPRNVRVAFILFKNV
                     LFSVHGEELPVFRLLRLRARRIPALIEDAKDVLLKLYDADAEYSADALEGIYDALEKV
                     SQRVLKEDVNDAMAAEALSAIAKEEDLNGRIRRNVMDTRRAVSFMMRSRLLNAEQFEE
                     ARQIMRDIDSLDSHTTFLFDKINFLMNATVGFININQNKIIKMFSVASVALLPPTLIA
                     SVYGMNFNAMPELSWRYGYPFALVLMVLSVAAPFIYFRRKGWL"
     gene            13861..14025
                     /locus_tag="PKV56_00785"
     CDS             13861..14025
                     /locus_tag="PKV56_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66368.1"
                     /translation="MNDKWLLVLGGLCVVLAVFGLKVWHAHGVCDEMSGRWLDGECHF
                     DERSADVKLR"
     gene            14025..15209
                     /locus_tag="PKV56_00790"
     CDS             14025..15209
                     /locus_tag="PKV56_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX66369.1"
                     /translation="MSSPAGEAPGLALQARLIALLRVACATTLMVLSFSMLQPVLAVR
                     LQTSGVSASAIGVLAMLPFLTVALMVPLMPAVFDRIGVAAAYRWGMGLEALATLGYAV
                     SEHYALWCALAVLGGIGAAAVWNGTEALIAHNAPPERRGRFTGLYQSALGAALAIGPF
                     LPGLLPLSPRALTVLASALMVAGLFTTFSREVSRLQAGHEDALPAGLWATICSVPGLV
                     AIAFAGGVFEAGLGSITTAYGSQAGMSLAAAASVAGVLGLGSFLLQYPCGWLSDHVSP
                     RRVFGLSGALLLAASGAFVAAAAWPPVLWICAFVWGAVGGALYTLTMIRVAHQFAASS
                     AVAGTAAMITGYTVGGAVGPSFSGVMLDFFGATGQATWLSLLAIAVLLLSRRFSGFAG
                     HG"
     gene            complement(15174..16277)
                     /locus_tag="PKV56_00795"
     CDS             complement(15174..16277)
                     /locus_tag="PKV56_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOX66370.1"
                     /translation="MKRVAVIGAGIAGLTAASGLLAGGWSVTLFEKSRGAGGRCATRR
                     SEAGAFDHGAPGFTATTDSFRAQVSRWRDSGWVAIEGDSGEVSGDPAAPSYAFGVPSM
                     NSLARQLAAHLPEGANWRPDTQIAAIEPAPGDAEAADGHGGWRLRLLNGDLEPARFDA
                     VVVAVPAEQAAVLLNLDAALSDAMRQTPSDPCWTVMAAWSAPWPVSEAVYRCADPRGT
                     LSLARRDDDRTGRTEVAGIGCRWVLHATPAWTREHLEAVPADVKAALLAAFGQHCGVH
                     HAVPQFSTAHRWRHARVPSPRHEPFGWNPVLRLGACGDAWHGATDSQSLQDDGIERAW
                     LSGRALAQRMVLEGVPLDRRVPSAVTGEAGEPA"
     gene            complement(16292..16744)
                     /locus_tag="PKV56_00800"
     CDS             complement(16292..16744)
                     /locus_tag="PKV56_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOX66371.1"
                     /translation="MKPGPIARSTDPRVARIIEAFETLTPADVSRLADFYAMDAAFKD
                     PFNEVRGVPAIQQVFTHMYVALEAPRFVIHDVIVQGDQCVLTWDFLFRFRRFSSDLQT
                     VRGASHLKLDAEGLITLHRDYWDVAEELYEKLPAVGALMRWLKRRANS"
     gene            complement(16754..17527)
                     /locus_tag="PKV56_00805"
     CDS             complement(16754..17527)
                     /locus_tag="PKV56_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX66372.1"
                     /translation="MSLNPRINEWTGRVVWLVGASSGIGLATARQLHDRGATVVVSAR
                     NAAAVEAFESSHPGSLGIALDATDRDAMKQAAERIVARCGRIDFALYCAGHYAPMRAS
                     SYDLDEMLRHERVNYVGALYLLDAVLPVLIRQKSGHLSLVSSVAGYRGLPNSLAYGPT
                     KAALINLAQTLYLDLHPLGIGVSVICPGFVATPLTARNEFKMPALIQPEEAAREMLAG
                     WAAGRFEIHFPKRFTRVLKALRHVGDGLYFRVIRRFTGL"
     gene            complement(17637..18170)
                     /locus_tag="PKV56_00810"
     CDS             complement(17637..18170)
                     /locus_tag="PKV56_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HOX66373.1"
                     /translation="MNRRSILAATAAATALLAACASPSPADYANEKPVLDLKAYFNGN
                     LTAHGIFTDRSGKVVRRFTVEMACSWQGDDGVLDERFVYSDGEKQQRVWRLKKLPGGQ
                     YTGTADDVVGTAQGRSSGNAFQWSYTLRLPVDAKTYEVQFDDWMYLVDERVMLNKAVM
                     SKFGVRLGEVTLAFHKP"
     gene            complement(18167..19513)
                     /locus_tag="PKV56_00815"
     CDS             complement(18167..19513)
                     /locus_tag="PKV56_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX66374.1"
                     /translation="MALQHVIPAADLSEHPSTTAATEPDGSPRDGPGLAGWRPGVRYG
                     ALGLPLAFVALPLYIVLPDHYAREFGIPLATLGAVLLGARAFDAVADPLIGRAADRLL
                     AGGARRAWWAIAMAALVLAIGFAALFFPPVEDRSALLVWCALALAVTYLGYSGVSVIH
                     QSWGARLGGDAPQRARIVAWREGLALAGVLVASVLPSVAGLAVTVGVLAALLFFAVLC
                     LRAAPQPAASAVVAAHLPMLLPFATPAFRRLLAVYLVNGIASAVPATLVLFFIRDRLQ
                     APAYEPLFLASYFAAGALSIPLWVRAVRWVGLERAWLLGMGLTIAVFSWATLLGAGDV
                     LAYTAVCALSGVALGADLTLPSAILAGVIQRAGHGEQAEGAYFGWWNFATKLNLALAA
                     GVALPALAWFGYEPGSRSPQALAALTAAYCMLPCLLKLAAAALLHTLLIRNLREPQ"
     gene            complement(19528..20118)
                     /locus_tag="PKV56_00820"
     CDS             complement(19528..20118)
                     /locus_tag="PKV56_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOX66375.1"
                     /translation="MRQPDLLDHTRRSLLALPLAIGCLPASVLAVARAALSPPPEVRS
                     ELPGARLHGSGRMKFLLFHVYDARLWTGPQFDADNFDQAPLALELEYARSFESTEIAA
                     RSIEEMERSGAIDAAKATRWLAAMKQSIPDVSEGDRVTGIQVPGVAARYFVNGKLTGE
                     IRDAEFTRLFFGIWLSKRTSEPRLREALLAGVRTGS"
     gene            complement(20120..21364)
                     /locus_tag="PKV56_00825"
     CDS             complement(20120..21364)
                     /locus_tag="PKV56_00825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOX66376.1"
                     /translation="MSQLDAMHTTTLSPGALPASAPAAARAVFRLLKNLKHGTLDIQL
                     PDGTGLHFGSAVDGGMRAAMRLLNWNVCGATMRSGDIGFAETYIAGDWTSPDPAMLIR
                     LMLANGSEVESLVYGTWWGSLLYRFRHWLNRNSRSGSKKNIHAHYDLGNEFYRLWLDP
                     TMNYSSAWFEGDRTRPMEEAQWAKVRRALTECDVKPGDRVLEIGCGWGSLAECAARDF
                     GATVTGVTLSSEQLEWACKRLADAGLPGDLRFQDYRDLTDGSAGPFDAVASIEMFEAV
                     GREYWPSFFETLNSQLKQGGKACVQSIVIRDDLFDRYVGSTDFIQQYIFPGGLLPSPA
                     EFRKAAARAGLRVVNELEFGLDYAETLLRWRQRFLSQEGPVRRLGFDSRFMRIWEFYL
                     AYCEAAFEAGNTNVMQFTLQRD"
     gene            complement(21402..22217)
                     /locus_tag="PKV56_00830"
     CDS             complement(21402..22217)
                     /locus_tag="PKV56_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HOX66377.1"
                     /translation="MSAAASPSTQRSGPMIGFGQVRHTRLRPAVNRFAYPTYFLMLPM
                     RRLRDAPHADLARNRFGLISFHDSDHGDGRADSLAWLDELLCSEGITDAGGEVWLHCY
                     PRVLGHTFKPVSFWYCHRADNTLAAVVVEVNNTFGERHCYLLHGAQLAYGKEQVATKV
                     FHVSPFCSVAGLYRFRFMHSRAPMAGPSERMQLTRTVARIDHDDAEGALIQTSVSGDL
                     LPLTSRSLRRAFFGMPLMTLGVIGRIHLQAARLWMKRVPFFRKPEPPIGFVTR"
     gene            complement(22214..23527)
                     /locus_tag="PKV56_00835"
     CDS             complement(22214..23527)
                     /locus_tag="PKV56_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX66378.1"
                     /translation="MPDHVRSVAVIGSGISGLSVAHALAPEARVTLYEAGSYFGGHTH
                     TVDVTLDGITHGVDTGFLVFNERTYPNLIRLFGELQVPTALSDMSFSVQVPDIGLEWS
                     GSDLNSVFAQRANLLRPAFWRMLRDLTRFNRLATTLAVRGEEAEQQQPIGEFLAEHRF
                     SAEFRDWYFLPMIGCIWSCPTDQMLRFPIATMIRFCHNHGLIQVTNRPRWYTVRGGAR
                     HYVEKLVSRVPDARLNTPVRSVRRMRATGQVSIGTDRGSESFDDVVLACHSDQSLALL
                     ADATPDEREVLGSIRYHRNRAVLHTDTSVLPSRKIAWAAWNYERARADSREATAVCLH
                     YLINRLQPLPFKTPVVVSLNPVNEPRADTVHGVYDYAHPVFDADAIAAQQRVPLLQGV
                     GNTWFCGAWTRYGFHEDGLMSGIGVVDGLRKRWAETATRPVPVAA"
     gene            complement(23929..24513)
                     /locus_tag="PKV56_00840"
     CDS             complement(23929..24513)
                     /locus_tag="PKV56_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOX66379.1"
                     /translation="MSTAQLIDFARKSCAAALLLFACASGAQAAECGTLLNRELPRLQ
                     DEKPQNLCQYAGKVVVVVNTASYCGFTSQYKGLESLHARYRDRGLVVLGFPSNDFGSQ
                     EPGSNQEIATFCANTFGVKFPMFTKTRVSQSAGAALSPLYAELRQRTGTLPRWNFHKY
                     LIGRDGSTVQSFSSDVEPDDKAFVEGIEKLLVAQ"
     gene            24669..25607
                     /locus_tag="PKV56_00845"
     CDS             24669..25607
                     /locus_tag="PKV56_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX66380.1"
                     /translation="MNEPARSLKSLTLSIAAAERDTGLTKDTLRVWERRYGFPRPDRD
                     GGGERAYPLEQVEKLRVVKRLMDAGHRPGRLVSMPIEELVRLSESTGDAPRRSAGSGP
                     LLDLKACLALMRSHDVDALRRQLLQTQMRMGLARFVIEVVMPLNTMVGDAWMRGQMEI
                     FEEHAYIECLHGLLRQALAGLPEAAPLDRPRVLLTTLPGEPHGLGLLLAQALLALEGA
                     RVVSLGVQTPVWDISLAAAAHRSDIVALGFTGCMNPNQVVDGLSELRSKLPPAVELWV
                     GGSAPVLHRRTVDGVMAFSSLEDIRPQIERWRHGSG"
     gene            25723..26598
                     /locus_tag="PKV56_00850"
     CDS             25723..26598
                     /locus_tag="PKV56_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOX66381.1"
                     /translation="MSTMLYPELFKQLEAVRWSMDKDIPWDRFDASQLSDEQAQTIKM
                     NAITEWAALPATEMFLRDNRGDSDFSAFMSVWFFEEQKHSLVLMEYLRRFRPDLVPTE
                     EELHEVRFEFDPAPALETLMMHFCGEIRLNHWYRRAAEWHAEPVIKAIYETLARDEAR
                     HGGAYLRYMKRALSQVGDEARVAFAKVGVLMASARRTAQALHPTNLHVNESLFPRDTV
                     QSRLPDPVWLEAWLDRQICFDAEWEGKVVDRILHNMSLLMERSFATVQELNRFRKEVA
                     ASIAAAAQSAAALPA"
     gene            26628..27101
                     /locus_tag="PKV56_00855"
     CDS             26628..27101
                     /locus_tag="PKV56_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOX66382.1"
                     /translation="MSDYTDKLCPRDEVESRVAALPQPVVFTNGVFDILHRGHVSYLA
                     QARALGGSLVVGLNSDASARGLGKGPGRPLNTQVDRACVLAALESVSLVIGFDEPTPV
                     ELLKRVRPQWYVKGGDYDIETLAETALVRSWGGDAQALPFVDGHSTTELVRRIRG"
     gene            complement(27159..27806)
                     /gene="rrtA"
                     /locus_tag="PKV56_00860"
     CDS             complement(27159..27806)
                     /gene="rrtA"
                     /locus_tag="PKV56_00860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HOX66383.1"
                     /translation="MPASAWTWVVIALSMAAVSATLHLMGGERTDSASHWAQALDWQP
                     ALARAEPWRAWTAVFVHLSDLHLAANLAGAVAVAAWGAVARVPARCSIAWFIAWPLTQ
                     WGLTMQPELRHYGGLSGVLHAGVSVVAVHLACSGTRAQRRIAAVVLAALAAKLLSEMP
                     WAGAISHPPGWDIAVAPGAHLSGALSGAMASALLEGVSRAVATVREALGMVDAHG"
     gene            complement(27834..30617)
                     /gene="glnE"
                     /locus_tag="PKV56_00865"
     CDS             complement(27834..30617)
                     /gene="glnE"
                     /locus_tag="PKV56_00865"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOX66384.1"
                     /translation="MGSLSSDIAANSRPESPAVGAAASPACADHSRFVQRIRRRYVDE
                     LPLLPEGLPRSAVITAVIEQLVAGGRSLASALRVTRQLVLERLAVLDIERGASMADVT
                     LAMTELAETTLDVALAQAMVEQDARSGPPLNAAGQRIDFWIVGMGKLGARELNVSSDI
                     DLIYVYEDGGETPGPQRLSAHEYFSQVVRSLYALIGETTEDGFVFRVDLALRPNGNSG
                     PPVVSLPMLEEYLQAHGREWERFAWLKSRVVAPRARLETTQIQGLRSMVNAFVYRRYL
                     DYGVFEGLRQLHRKVRDEAQRRAAGRPERANDVKLSRGGIREIEFIVQLLLVVRGGQF
                     PEIRTRSTLKALDKLAARGLMKPASAARLAEAYVFLRRLEHRIQFHDDQQTHLLPTSD
                     ADLRWIAGSLGLRCTVDACELLDRLCETREFVATEFDALLHEGQPAPAQVNGVACRGC
                     VPSLQPVDSGEFLAKLPEELAARVKPWCDHPKIKVLRDESKARLARLVLRAADAVKDG
                     RCTIAAAARFIDWLEPLLRRESYIALLAERPEVQNRLLRLLGLARWPIRYLMLHPGVV
                     DELADERLLHGRFDATEFTADLEARYAAWGRSGQVDEEALLDALRRAHHSEVFRTLVR
                     DVEGHISVEQVADDLSALADATLACALRWSWKHLKQRHRDQPQIAVIAYGKLGGKELG
                     YGSDLDVVFLYDEAGEPDPDKASEVYAAFVRKLIGWLTLRTAAGELFDIDTALRPNGG
                     SGLLVTSIASFDKYQTGRGSNTAWTWEHQALTRARVCAGDAALVAPFDEVRRTVLAAP
                     RDPEGLRTEVRAMREKVGAAHSVKAEMFDIKYSPGGMMDVEFAVQVLVLEHSGRHPEL
                     IPNVGNIALLQRAESAGLLPPTVGSAAADAYRDLRRAQHAARLDEQPTQVPLDAMPAQ
                     RAAVLALWHAVFG"
     gene            30761..35071
                     /locus_tag="PKV56_00870"
     CDS             30761..35071
                     /locus_tag="PKV56_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HOX66385.1"
                     /translation="MPVQEVDVSVLSPGTRLTLARLLIRILKGVGLLLIAAWSLLILA
                     WATAHWFILPHIDQWRGPIERQATQLIGQPVRIGAVRVTSSGLVPAIELKDVTLLDAQ
                     ARPALRLPSVLAALSAHSLLSLEPRFAQLLIDGAHLEMHRDAAGRLFIAGLDFNGPGR
                     NDRASANWFFRHHEFVIRGGSVRWTDELHPAPPLELSEVQVVVRNSLRHHDVRIDATP
                     PAHWGDRFSVTGRFTQPLLAESADWKRWRGTVHATLPGVDVRTLRQHATLPFDLTQGQ
                     GALRAWVDVVDGSVAAATLDVALREVALRLQPGLDPLGFAEVTGRLAGRQDERDTELT
                     VQQLGFVTSDGVRWPASNAALKWRRDGSGAVAGGELTAERLDLALLTQVVRRLPVGDG
                     AHRLLADFDPHGTASEFSLRWDGVPNPVAGAAAASAASAADAVALPPRYQLRVNLSGL
                     SLAAAPAADAQTAGRPGLRQADLRVSADQSGGEVRISMTKGEIDLPGVLEEPTLPFDR
                     LSAQLNWRVEASAAAAEPSHVTVQVKEAQFANADLQGELRGSWSTGLFAANGKHSPRY
                     PGLLDLDAKLTQVTAARVFRYLPLGVSQEARRYVERAVHAGRVASATFRVRGDLNDFP
                     FAQGNAKDSEFRIAAQIDKASFAYVPSWPATVTEPAYVSTWPELEELTGELVFDRESM
                     LIRNAQARIQGVKLGGVQGGIKTLTDKSELVLEGAGRGAAADMVRFVNVSPVGGWTGN
                     ALAHSTASGSADLKLSLAVPLADPAAATVRGSVTLAATDFRLRPDTPQVGAVRGRVDF
                     SQKGFSIVGASGRLLGGDLSVDGGLQPDGSMRFVGQGIATADGLRRGGDLGGAALARL
                     ANSLSGQASYRVALNLVRGQSDLLVTSNLVGLGSELPPPLRKAADSPLALRFQSAPVT
                     DAAAGSGSASATALQDSVRLDLGNLLQLHYVRDVSGALPRVLRGGIGVMEPAPTPANG
                     VSALLNLGNLNVDAWEQVAASLTGPAAGGSGNAGGSAMLGPSGGDAASVFSAYLPTTI
                     GLRAQELHSGSRHLSRLVAGLSQEGTAWRVNLDSDQASGYIEYRAPRASSGSAAAAPM
                     SAAAAGRVYARLSRLSLPKDDVADVENLLDQPAAQSVPALDIVVDDLELRGKRLGRME
                     LEATNRQGAREWRLTRLALTTPEAKLTASGNWAALGAGAGAASGAVSPAARRVVLSFK
                     LELSDSGALVDRLGFGKVVRGGKGGLSGQVAWFGSPLSLDYPSLTGQINISIAAGQFL
                     KAEPGVGRLLSVLSLQALPRRLVLDFRDVFQQGFAFDDISGDVQVTQGVAQTNNLRMR
                     GVQAAVLMEGKADIEQETQDLRVIVVPEIDAGTASLAYAAINPALGLGAFLAQALFSK
                     PLIAANTREFHITGTWGDPKVDKVDRKFGAPVPDIPAPSSSMPSGAPAAAANESPPKP
                     "
     gene            35068..35901
                     /locus_tag="PKV56_00875"
     CDS             35068..35901
                     /locus_tag="PKV56_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOX66386.1"
                     /translation="MKIAALQMVSTPCVEHNLATAGRLIAQAADAGARWVALPEYFCL
                     MGHSDRDKLAIAEVAGAGPIQDMLSGAARRHGVWVIGGTLPIRCDEPEHVFNACCVYA
                     PDGTMAARYDKIHLFCFDNGRERYDEGRVLRAGSEPVAVSGSADGDIAALRVGLSICY
                     DLRFPELYRALMTMPGQPPCDLIAVPAAFTHTTGQAHWELLLRARAVENQCYVIAAAQ
                     GGLHENGRRTWGHSMIVDPWGEIVAVHAEGEGVVIADLDLDRLAAVRLQLPALKHRRI
                     T"
     gene            complement(35917..36411)
                     /locus_tag="PKV56_00880"
     CDS             complement(35917..36411)
                     /locus_tag="PKV56_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66387.1"
                     /translation="MARPERTRTAPPAQGPRKPQASDRMLPAYVLAGVILVIGVPAAV
                     MLGVSWVRVSEPTPSGQAAPNWVRSESVQATTSDGESVKARVAIDASDRSSRASLEEN
                     RTQVAMMLQICLGTHDRPTLQRAGGMNKLSKDMERRLNEFLAARHEAPVRAVVVQDLL
                     FSKV"
     gene            complement(36576..38093)
                     /locus_tag="PKV56_00885"
     CDS             complement(36576..38093)
                     /locus_tag="PKV56_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOX66388.1"
                     /translation="MNAPEPALHRPEGGAPDQATAIAAFCDRAWDEAIIPALVDYIAI
                     PAKSPMFDAEWQSHGHIERVIRDAAQWVERQAVAGLKLEVVRLPGRTPVIFFDAPATR
                     PGGSASDAGTVLMYGHLDKQPEFTGWRHDLGPWTPKYENGLLYGRGGADDGYAVYAAL
                     TAIRALDAQGIPRPRCLGLIESCEESGSYDLPAYIDALKDRLGRVDLVVCLDSGAGNY
                     DQLWLTTNLRGMVSGVLKVEVLTEGVHSGDSSGLVPSSFRILRHLLDRLEDSRTGELL
                     PEGFHCEIPADRVEQARATAAALGDELWKRFPWACGADDGPTLPTTSDPFEALIRRTW
                     KPTLSVTGVDGFPEMRSAGNVLRPYTAFKLSLRLPPLIDGNEAAARLKALLEDNAPYN
                     AKVTFSADGRSGGGGGGQPSGATGWNAPALAPWLERALNAASRTQFGAPCGYIGQGGT
                     IPLMSLLQQGFPDAQMMVCGVLGPKSNAHGPNEFLHVPYGKKLTAAVAQVIAACE"
     gene            complement(38130..39674)
                     /locus_tag="PKV56_00890"
     CDS             complement(38130..39674)
                     /locus_tag="PKV56_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOX66389.1"
                     /translation="MARPHEQRSLFGEILDWMLAPLLVLWPMSVVLTWLVAQGIANQP
                     YDRELADMVRVLSKQVVVSSPQGYAAATFELPAGASEILRADEADDVYYQVLGLRGEY
                     LSGDSTLAVPAEEDRVPPGELRYRDDTIHDENVRVAYMWVDVPGRPADAAPLVQVAET
                     LGKRSRLATEIIKGVILPQFVILPLAVLLVWLALARGIAPLNELQQRIRKRDSSDLSP
                     IDEREAPEEVSPLVRAINDLLARLDRSISSQKHFLTDAAHQLKTPLAGLRMQAELAQR
                     DIDAGKNDPADIKRSLQQIALSSQRAAHTVNQLLSMARADDKEQALNLGPVDLAELAV
                     ETVRDFVPRALEKRIDLGYEGPGRDVEGSLEAPATGGLKVRGHRVLLGELIRNLVDNA
                     LRYTPSGGTVTVRVIDDPFGQVVVLQVEDSGPGIPPAERDQVFQPFYRVLGTQVDGSG
                     LGLAIVRQIAAQHGAELLLEDANLRHRSGLNDQAGAPFGPGVRFTLRFKAMHDQPIEA
                     DGAVLH"
     gene            complement(39691..40362)
                     /locus_tag="PKV56_00895"
     CDS             complement(39691..40362)
                     /locus_tag="PKV56_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX66390.1"
                     /translation="MRVLIAEDDQVLADGLLRSLRNAGYAVDQVNSGSDADAALASHE
                     FDMVILDLGLPKVHGLEVLRKLRARGSSVPVLILTAADSVDQRVKGLDLGADDYMAKP
                     FSLQELEARVRALTRRGLGTASSVIKHGPLTFDATGRVAYINDQMIELSAREISLLEV
                     LLQRAGRLVSKDQLVERLCEWGEEVSNNAIEVYIHRLRKKIEQGPVRIATVRGLGYCL
                     EKIAA"
     gene            40589..41665
                     /gene="recA"
                     /locus_tag="PKV56_00900"
     CDS             40589..41665
                     /gene="recA"
                     /locus_tag="PKV56_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOX66391.1"
                     /translation="MDAPVKALNTEKAKALQAALAQIEKQFGKGTIMRLGEGEVIDDI
                     EVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGTCAFIDA
                     EHALDTAYAQKLGVNLQELLISQPDTGEQALEIVDALVRSASVDLVVIDSVAALTPKA
                     EIEGEMGDSLPGLQARLMSQALRKLTGTIKKTNTMVIFINQIRMKIGVMFGSPETTTG
                     GNALKFYASVRLDIRRIGSIKKGEEVIGNETKVKVVKNKVSPPFKTAEFDILYGEGIS
                     REGEIIDMGVEAKVLEKSGAWYAYKGEKIGQGKDNAREFLRENPALAFEIENLVREAV
                     GIPSLPPLDGSDAPKAAKERPVKE"
     gene            41696..42307
                     /locus_tag="PKV56_00905"
     CDS             41696..42307
                     /locus_tag="PKV56_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOX66392.1"
                     /translation="MKPPLSLKGRALQWLAQREHSRLELRRKLLQRLRDDHRHDCRPP
                     DGEEDNGLTTDAAASSNHPEVNPEIEVDALLDWLQSHRYLSDERFVESRVHARSARYG
                     NLRIRRELSQHGVALTSALDGDLRGSEQARAVEVQLRKFGGPPASLVERARQARFLAS
                     RGFSSDVVQKALRAPRGRDAAVTTAGEDASAVRLHSVPDDDAD"
     gene            42598..43758
                     /gene="sucC"
                     /locus_tag="PKV56_00910"
     CDS             42598..43758
                     /gene="sucC"
                     /locus_tag="PKV56_00910"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOX66393.1"
                     /translation="MKIHEYQGKELLSRYGIPVPKGYAAFSVHEALEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSLDDVSKLAGEILGMQLKTHQTGPEGQKVRRLLVEEGA
                     DIKHEYYVAAVTDRATQKVVLMASSEGGMDIEQVAHDTPEKIIKVFVDPLIGLTDAQG
                     LQLAAGIGIPAASQSQAVDVFRKLYTCYMETDSSLAEINPLILEGSGNIKALDAKFNF
                     DSNALFRHPEIVAYRDLDEEDPAEIEASKFDLAYIQLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATAEKVTEAFKIMLGNPKVKAILVNIFGGIMRCDTIAEGVIA
                     ACKAVNLNVPLVVRMKGTNEDIGKKMLKDSGLPIISADSMADAAQAVMAALK"
     gene            43784..44677
                     /gene="sucD"
                     /locus_tag="PKV56_00915"
     CDS             43784..44677
                     /gene="sucD"
                     /locus_tag="PKV56_00915"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOX66394.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTRMCREYANGKNCFVAGVNP
                     KKAGEDFEGIPIYASVKEAAQSTGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNKMIQKEAKGGKKTLLLGPNCPGLITPEEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDIMRLFNDDPDTDAVIMIGE
                     IGGPDEAEAAVWCKANMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAVME
                     ACGFTVTRNPSEMAKLLQKLL"
     gene            44677..46014
                     /locus_tag="PKV56_00920"
     CDS             44677..46014
                     /locus_tag="PKV56_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOX66395.1"
                     /translation="MPETRRASAIHAPPPGAPLARALLGHPVLDAEFLRDPYPTYRVL
                     REGAPLVWSDEFFGGAWLMSRHSDVEMLFRDARFSAQRAGGWVMRTEESRTELRGFQR
                     LFARALVFLDAPDHTRVRQVLATGFRPSDLQRLAPRIEQAVAERLDAVTPARLFDFVA
                     SVARPLPAQVIGMLMGIDDEPSGFVAWSDALADFVGAPDPSQAQALTAQAALLEMARY
                     FESLLTRRRAALDGGDEPRDDLIERLLQAERSGQIHGSAELMAQCVMLLFAGHETTRH
                     LLANGLRMLLAHPDAWHHLRRDRALMPGAVRELLRFDSPVQYTGRRVVTDLVLHGQTL
                     RRGDLVVALIGAANRDPARHERPDELDISRSAGAPLAFGQGPHACIGAALTLLEAQTV
                     FSQVIDRWPDLALAEPNAAAWTGKPLYRGLTELRLNSAGPSSRSADVGAYTAA"
     gene            46042..46764
                     /locus_tag="PKV56_00925"
     CDS             46042..46764
                     /locus_tag="PKV56_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOX66396.1"
                     /translation="MQALMTPEFWIAVGQIIMIDILLGGDNAVVIALACRKLPPAQRT
                     KGILWGTAGAIVLRVILIFFALQLLSIPFLKLVGAILLVWIGVKLLLPEDEDGHAQVE
                     GSDRLWGAVKTVIVADLVMSVDNVIAIAGAAETAGGDHTMPLVIFGLLVSIPIIVWGS
                     QLVIKLMDRYPVIITFGAMLLGWIAGSMAVSDPAVVNPEVLPQLPKLIVSDGVKYGAG
                     AAGALLVLAIGKALQARRGVRG"
     gene            47154..47945
                     /locus_tag="PKV56_00930"
                     /pseudo
     CDS             47154..47945
                     /locus_tag="PKV56_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            48183..48965
                     /locus_tag="PKV56_00935"
     CDS             48183..48965
                     /locus_tag="PKV56_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOX66397.1"
                     /translation="MPFEFFSATDTGRARNNNEDSVAVDAVSALVVLADGMGGYNAGE
                     VASNMATSFILTELGRWLTEAASNATDADVRRAMDICVDNANRAIFNAANSNPQYAGM
                     GTTLVVGVFREGRLLLGHVGDSRGYRLRDGVFTRITHDHSLLQEQIDAGLITEEQAAF
                     SSNKNLVTRAVGVEDAVMLEAHLHDLLPGDLILLCSDGLSDMLDDERIGALLQSCESI
                     ESGARTLISAANEAGGKDNIAVILVRVQGGAGAGRSWWPFKR"
     gene            49044..49697
                     /locus_tag="PKV56_00940"
     CDS             49044..49697
                     /locus_tag="PKV56_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOX66398.1"
                     /translation="MGKLVVSLDGVVIKEVQITKDKTTLGRRPYNDIVIDNLAVSGEH
                     AVLQMLGADVFIEDLNSTNGTYINGKAIKKQLLVHNDTVEIGKYKIKFLVADGTDYEA
                     TQIMRPASHAAAAPVPPGLQPASTFGSLGASASIKVLNGAAAGREVSLTKVVTTIGKP
                     GVQVASITKRPTGYVFAHVEGAARPTVNGNPLVAETTQLKDGDVIELAGTQMQFINR"
     gene            49899..50696
                     /locus_tag="PKV56_00945"
     CDS             49899..50696
                     /locus_tag="PKV56_00945"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HOX66399.1"
                     /translation="MTLRVLGCSGSIAAGCKTTSFLLDDDVLIDAGTGVGDLPLDALA
                     RIDHVLISHSHLDHVLSIALLADSVMRVRAAQGRGPIQVHALPETLQALHQHIFNGVI
                     WPDFTRLPTPDRPALQLVPFSVGEVLTVGARLRRVEVLSAAHTVPAVGFAVEGTQSDG
                     SICWWVYTGDTGPNPALWNRLRSMNVSHLVIETAFSNEEQQLAQLSSHLCPTTLAREL
                     VQLAGRVDVRISHIKPGEMFSVMSEIGRLAMRHRVQALAIGAVMSLG"
     gene            50915..51388
                     /locus_tag="PKV56_00950"
     CDS             50915..51388
                     /locus_tag="PKV56_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOX66400.1"
                     /translation="MQRRIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEV
                     VLAGSACRTTITEVYQSSDSGASVGANSWGCETDASAAAGQSKYVASVVTDDDGMVTI
                     TAQGIPQVDGQTVELEPQDSTGTKMTVATNIGQAVFQWKCRPGTMTAKYLPGSCR"
     gene            51466..53133
                     /locus_tag="PKV56_00955"
     CDS             51466..53133
                     /locus_tag="PKV56_00955"
                     /inference="COORDINATES: protein motif:HMM:NF023274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HOX66401.1"
                     /translation="MFELLGSAAMVIAWLVPDHYLPWLSAYNDSVMALALILLLLGLV
                     KREASQASVPAIFWITVAVAGIPWLHWVTGRLAFSGDAVVGSLYVLALAIAILVGHGW
                     ARRDPTGSAALLAGTILLGGSLSAVIALFQGLRVGDLGLWSLGLCTGGRACANLAQPN
                     NLGTLLGLGVLGLLLLREQRRLGRGVSAAVLALLLLGAATTQSRTAMLFGPAILIGLF
                     VARRRGVQFQIQLRTVFAVTVIHWLLSYGWPVLQTALLLDTTASLSARSLDAGRLQAW
                     VMFIDALSQSPWTGVGWMQTASAQLAVAENHPPLRLVWQHAHNLFLELMLWCGYPLGL
                     ALTLLIVTWYLNRAKRVDSIEAVVGLLSVTLFGIHSMLELPYHYAYFLIPIGLWIGHV
                     EHACGSGIRRFAAWRWLPAAAAAVMVLGIWRDYPKIEEEARRVRFENLRIGLTTAAQP
                     TPDAPFLSGLTAFLEFTRTKPSEGMTVADLKAMENVVKRYPYAGSIAKLSWAWALNGR
                     LPEAIEMFKKIRYVHGDVLYHRIRNDMHQRVVDGQIGLRALDDAVQD"
     gene            53209..53976
                     /gene="tfpZ"
                     /locus_tag="PKV56_00960"
     CDS             53209..53976
                     /gene="tfpZ"
                     /locus_tag="PKV56_00960"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HOX66402.1"
                     /translation="MASDFLPIRIKAAGLHLLASCLVAALSAALVFLVWYPSPYATMA
                     GGSGLFLLIVSIDVILGPALTAVVANPNKKRSELARDLAVIVTVQLAAFGYGLYTMAL
                     ARPALLTFEVDRLRVVTAVDVDPVLLKEAPPGLQSLSWTGPRLIAAVKPTDPDEQMRS
                     IELGLAGIDLAMQPRQWRDYAARADEAWRVARPVSVLLSQYPQLAADVARIARSAGQP
                     TESLRFLPLMSRQASWVSLIAAPGARVVGHLPVEGFF"
     gene            54161..55960
                     /locus_tag="PKV56_00965"
     CDS             54161..55960
                     /locus_tag="PKV56_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HOX66403.1"
                     /translation="MKRGPASPPGSPWLTIAACLAVVSPAVLAVNLPPSATFLNQAAA
                     VSGWGLFCAVLAWSLVPGPSAPSMRWGVGLRALLAAVALLMAACAGSWMMTGLPSSLT
                     LSAIGMLAAAALVACVGAAAMSAGQGAVAFRAFCIALVMAGILSAAIGAVQVFAPQGA
                     DGHWIAISALEGRASGNLRQPNHLSSVLLWSVVALLWLAESMGTGCLSARPVRRVGLY
                     LGLLALIFGLVLSASRTGMLGVLALAIWGALDRKLSRRTRVLLCASPLIYALCWVGLT
                     AWADASGHVFGGAARLRSEADISSSRFGIWADTLELIAKRPWFGVGWGEFNLAWSLTP
                     FPGRPVAFFDHTHNLLLQLVVELGAPLALLVTALLAWSLWRAWKAGCTSVAGGDHPPA
                     ALVRSAWMMVLLVAWHSLLEYPLWYAYFLLPAAFAWGLCLGAESPTAPSPKPASAART
                     PAWSLWLASMAMTAAGAASVIDYVPVVAIFEPGDDAPPLAERIAAGRRSVLFSHHADY
                     AAATTRAAPAEVLLAMQGAKHFLLDTRLMTAWAQALADSGDIERARHIAARLREFRNP
                     ASREFFAPCADGSMPPPFQCTPPTTVMDERAFR"
     gene            complement(56016..56567)
                     /gene="moaC"
                     /locus_tag="PKV56_00970"
     CDS             complement(56016..56567)
                     /gene="moaC"
                     /locus_tag="PKV56_00970"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOX66404.1"
                     /translation="MTAPAEPAPPMPEEASPLTHFDATGQAHMVDVAGKAETHRIARA
                     GGEIRMLPTTLALIEGGQAKKGDVLGVARIAAILGAKRTAELIPLCHPLPITRVAVDF
                     EIDRATSRVICTAQVETLGRTGVEMEALTAVQIGLLTIYDMCKAADRGMVMGQIRVLE
                     KQGGKSGTWSIEGNAAAGADSGQ"
     gene            56805..58451
                     /locus_tag="PKV56_00975"
     CDS             56805..58451
                     /locus_tag="PKV56_00975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOX66405.1"
                     /translation="MLVGLLCVSLLGGTWSPQAQAQVNLPALGDTASEDFNLGTERHL
                     GDAIMRDIRRDPDYLDDPILLEYLQSLWQPLLAAARLRGDLGDDLNQRFSYQPFLVRD
                     RSVNAFALPGGYMGVHLGLMAITQTRDELASVLAHEMTHINQRHIARGFANSKKQSLI
                     SAAAMIIGVLAATRSGGGDAANAMIAGGQAVGIQGQLNFSREMEREADRIGSQMMASA
                     GFEPSGMASMFERLQQSSSLNDSGGYPYLRSHPLTTERIGEARARLGTAGLTSAPPLV
                     SSPSTALEHRAALARARVLMDTRVDALRRWQALDGEGSAASATLGERFADAYSSALAS
                     SLLRDWVRADASMSRASDIARGGSAAVASISDVAATGALPQRDARGERSVALLKVESF
                     LHRGEPARAQAILAPLTRSVGPVAAEAATTSRPVMVLAAQVALAEGRSKENAALRASA
                     DQLQTWVARYPDDAEVWSMLGRVSAQLGWALRSVRAEAESRYASGDLPGAIDRLKAGQ
                     RLARSSTTAVDFVESSVIDSRLRSIEAHRRQLLTEQLGYQ"
     gene            58531..64524
                     /locus_tag="PKV56_00980"
     CDS             58531..64524
                     /locus_tag="PKV56_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOX66406.1"
                     /translation="MTSIPAEAATIVTVSPRGEVAQVRQLAVTFSEAVVAFGDPRLPD
                     PMAVSCTGALARGSGLWTGDRHWVFNFEEPLPPGVRCSAQVRADWKPRVAGTDAALTG
                     TSSFSFSTGGPAVESIEPYDGSTIEEDQHFLINVNGAATERSVATHAWCEVEGLGDRL
                     PVRIVTGALRERIVAAAGPRALKDAKREAERQSRLIVLTCPRPLPTDTPMRLVWGRGI
                     AAVVNPGVATRVEQRFGYRVRPAFKAEFSCERERATAPCLPIRPLVLRFSAPIDRKLA
                     EQVRLVPAGGKPIAPVFDKDDKDPEVSSITLAMRVAENAAYTVELPSSLKDAAGRVLA
                     NAGAFPMKVSTGDAPPIAKFPAAPFGIIERFGDAANPGAVSAPTPMLPLTLRHVQGDL
                     RSSAPGGAVRVKRLQTDAEMLAWYDKLHRFHESDLSAQEAGLPKAQWFTTESLVDERG
                     RSTRQQVERRVQTRTLSLLKADTSAKRLDLPQLKGGDPRPFEVVGLPLAEPGYHVVEI
                     ESARLGQSLLEPRAPMYVRTGVLVTNLGVHVKLGRENSAVWVTSLDRGRPVEGADVAI
                     NDCFGKRLWAGRTDARGLAIVKLALDLQVGECPNEAGYFVTARKADADARTPQVIDTA
                     FVFTSWQKGIESWRFNLPTSNASERDAAAHTVFDRTLLRAGETVSMKHFLRAETLAGL
                     APWDAASLPTRIKIVHQGSGQQIVQPLVWNGTRYAVSTWNIPPAAKLGLYEVMLERDV
                     VNERHRSSWSSGSFRVEEFRLPLVDARLSGPSQVPVAPRELPLSVQMAFLSGGAMAKS
                     PAAVSALLKPRVPAFPGHDGFSFEPPRVAAADGDSEDETASAQGADRLVADKLPLTTD
                     AQGAATVLLKDLPPLTRASELHSELTYTDPNGEIQTVSSRIDLWPSAVVLGIKTRAWA
                     SQRGGSVQFNVLALDTRGQPIRNQRVEVRGRLSQTLSNRTRIVGGFYAYDNRTELKDL
                     GVVCSGSTDERGGLACDATLSAAGQVELIAQAKDAAGRTAQAATGVWITKQGELWFGQ
                     DNDDRIDLLPEKPRYEPGETARLQVRMPYREATALVTVEREGLLDTRVVTLSGDDPTL
                     SIKIEKSWGPNVYVGVLALRGRLREVPWYSFFTWGWKEPLNWARSFWYDGREYQAPTA
                     MVDLAKPSFKFGVAALQVGIAAHELRVSVTTDRAQYAVRQKVQARIKVTQSANGERPA
                     PAGTEVAFAAVDESLLELRPNESWDLLNGMIRSRAWGVETSTAQGEIIGRRHYGRKAV
                     AAGGGGGRGGTRELFDTLLLWKAVVVLDARGEATVEVPLNDSLTRFRLVAIADGPLDA
                     ATPTFGTGSTTVRVTQDLQLLAGLPPLVRGGDRFGALLTLRNTTAHEMKVRATLAGTA
                     NRGTAEIVRTPLAFAPQDVVLPAGSAKELAWPVEVPIDAFSIVWDAAVETVAAAGAAP
                     AKDRIRLTQLVAEAVPVRVMQATIARLDGPYSLPLAAPADALPESGVKRGGLRVSLQP
                     RLGSALPGLRRYFETYPYSCLEQKAAKALGLGDAALWGTLVETLPTYLDADGLAGYFP
                     PRAGDASAGNDRLTAQLIATSHEAGFALPDAARNAMLEGLSAFVEGRIERRLWAPASA
                     QGLNLAVRKLAALDALARHGRVQARMLGSINATPMLWPTAAVIDWLNLLRRLRAQDIA
                     VPDHARRIEQAQQILRSRLALGGTTLKFSDEVGDHWWWLMDSADANAARLILAVLDEP
                     AWRDDLPRLVVGALGRQRDGAWSTTTANLWGAIALDKFSAKFESQAPTGSSAVRVDSR
                     PSGASAAAAPPALASGIVDWAKQPAGGSVMLTWPAEPATLSVRQQGSGTPWLTLQGLA
                     AIAPKGPVRAGYGLTRHITAVSRKDPSRWSRGDVLRVRLEIDAQTDMSWVVVSDPVPG
                     GATLLGSGLGRDSAIAAQSRSGESDTDAGSAWTAYDERSFEAFRRYYGQLPRGRHVVE
                     YTLRLNAAGRFALPPTRIEAMYAPETFGELPNGAIDVAP"
     gene            64521..66683
                     /gene="pbpC"
                     /locus_tag="PKV56_00985"
     CDS             64521..66683
                     /gene="pbpC"
                     /locus_tag="PKV56_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOX66407.1"
                     /translation="MKRIVWAAALLLALEAVAVVPRFEDVRARHSPSDTTLLDRQGVP
                     IQTLRTDSSVRRLPWLPLSEMSPALLQAIVFSEDRHFHEHSGVDWAALASSAWANAWN
                     TRTRGASTLTMQLAGLLDARLERPVGGRSMTQKLGQIVRARELEARWSKAQILEAYLN
                     SVAWRGELVGINALSQTLFGKRASGLDTHEAAVAAALVRGPNASAAVVTQRSCGVLQA
                     QGMSCAGLSALVELALVRRGGPVLGEQLAPHAARYALASVRKPPATLRTTLDASTQRV
                     ANAALRQQLVELAGRQVEDGAVLVLDNASGEVLAWVGSSGELSDAAQVDGVLARRQPG
                     STLKPFVYGLAFERRLITPASLLDDSPTQLATATGLYLPQNYDRAFKGWVSARTALAA
                     SLNVPAVRVGAMLGADALWARLNAFGFELPESGGYYGDSLALGSADVTLLALTNAYRA
                     LANGGMLSPVMLRRSSTTSPDASVAPRVASKRVANAAATHLVTDILSDNNARALTFGL
                     DSALALRGPVAVKTGTSKDLRDNWCIGYSDRYTVGVWVGNASGAPMHDVSGVSGAAPV
                     WRAVMQHLHEGVPAKQRAVPAGVVIARTRFEGVREPARDELFMAGTEVTRPRPDMQGA
                     IGMTRAASAFGIRSPREGSLYAIDPDMPPRAQRITFEGEAGTWVLDGQVVGRGQRLHW
                     TPWPGRHQLTLRGREGQTLQAVRFEVRGAAVKSASAAR"
     gene            complement(66690..67046)
                     /locus_tag="PKV56_00990"
     CDS             complement(66690..67046)
                     /locus_tag="PKV56_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOX66408.1"
                     /translation="MKIIVRWLLLAAALLLVAYLYPGVSVQSFGSAMLAAFVLGLLNT
                     LLRPVLVLLTLPVTLVTLGLFLFVINALMFYFAANLLSGFNVTGFAAALIGSLIYSLC
                     GMVIDVAMERIFSPKS"
     gene            67399..>67747
                     /locus_tag="PKV56_00995"
     CDS             67399..>67747
                     /locus_tag="PKV56_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOX66409.1"
                     /translation="MATAKKAPAKAAPAKKAAPAKKAAAKTVTAKSAATKTAAPAKKR
                     SPNAAFMKAVTPSPQLAAIIGSTPMPRTEVTKKLWEYIKANKLQDATKKTNINADAKL
                     KEIFKKAQVSMFEM"
BASE COUNT        11116 a        22158 c        22702 g        11771 t
ORIGIN      
        1 actcgatgcc gccctgctcc gtcttgcgcg agtagatgtg gttcagcacc atgccttgtg
       61 tcagcagctt ggtgaaaggc tcgtcgactg acaccagctt caggtcgcgc atcaccttgg
      121 tccagaagcg cgcgtacagc aggtgcagga tcgcgtgctc gatgccgccg atgtactggt
      181 ccatcggcat ccagtacgcg gcgccgtcgc cgaccattgc gtcggtgttc ttcgggtcgc
      241 agtagcgcat gaagtaccac gagctgtcga cgaaggtgtc catcgtgtcg gtctcgcggc
      301 gcgcaggcgc gccacacacc ggacaggtca cgccggcgtg gaagcccgcg tgcttgtgca
      361 gcgggttgcc gctgccgtcg ggaatgcagt cctcgggcag caccaccggc aggtcgcatt
      421 cgggcaccgg caccgcgccg tgtgtctcgc aatggatgat cgggatcggc gtgccccagt
      481 agcgttggcg gctgatgccc cagtcgcgca gtcgccaggt ggtcttcttt gctccgaggc
      541 catgagtttc cagtgccttg gcgacggcat cgaccgccgc cgcgcaactc aggccgctga
      601 agtagtcgga gttaatggtc acgccgcgtt gcttgtcggc gtaccagtcc tgccagcgtt
      661 cgtaatcgaa atgcggctcg ccatcgacat gcacgacctg caggatgtcg atgccgtatt
      721 tgcgggcgaa tgcgaaatcg cgctcgtcgt gcgccggcac gcccatcacc gcgccgtcgc
      781 cgtagctcat cagtacgtag ttgccgaccc acacctcgac cggctggtga gtcagcggat
      841 gctgcaccgt caggccggtg cgcacgcccc gcttctcctg cgtcgccagc tcggcctcgg
      901 tcgtgccgcc agcctggcat tcggcgatga attcggccac cgccgggttc gacgcggcag
      961 catgaacggc cagcggatgt tctggcgcga ctgcgcagaa ggtcacgccc atcaccgtgt
     1021 cggcgcgggt agtgaaaaca tagagacgac cgtcctggat cgtcttgccg ctgtggtcgc
     1081 tgatgtcgtg ggagaacgcg aagcgcacgc cctcgctctt gccgatccag ttctcctgca
     1141 tcaggcgcac ccgttcgggc cagcccggca ggtggtgctg cacctggtcg agcagttcgt
     1201 cggcgtactt cgtgatgttc aggtagtaac ctgggatctc gcgcttctcg acgagggcgc
     1261 cgctgcgcca gcccttgccg tcgatgacct gttcattggc cagcaccgtc tgatccaccg
     1321 ggtcccaatt cacgacctgc gtgcgacgtt cggcgatgcc cgcctcgagc atcttcagga
     1381 acagccactg gttccagcgg tagtagtcgg gagagcaggt ggtgacctcg cggctccagt
     1441 cgatcgccag acccatcgcc tgcatctggc ccttcatgtg ggcgatgttg tcgtaggtcc
     1501 acttggccgg tggcaccttg ttcttcatcg ccgcgttctc ggccggcagt ccgaaggcgt
     1561 cccagcccat cggcatcagc acgttgaagc ccttcatccg caactgccga gtcaacatgt
     1621 cgttgatggt gtagttgcgc acatggccca tgtgcagttt gccgctggga tacggcagca
     1681 tcgagcaggc gtagaacttg gggcggctgg tgtcttcggt gacgcgataa gcgtcgcgcg
     1741 cgctccaggc ggcctgcgcc gcgcgttcga cctcggtcgg cttgaattcg ggattcatga
     1801 gggggcgcgg tgcgctcggg gacgtgtctt tcgattatag gaagcaggct ggcgccaact
     1861 cactctgccc ggcgcgccca gccagcagtt gaccgtgccg ctgcgacggg attttcagcg
     1921 gccatacact gcgcaggctt gtcggggcgc cgcagacgaa agtcgaggct gagaccactt
     1981 tgatgtggaa cccgctgaac ctgatcgggg tcgtgcccgc gtaggaatca agtgcaaagg
     2041 tcaccgtgcg gtgggaattc ctcccgccgt gtcggctcca ggcaagcgtt catccgtcac
     2101 gccaccagga gctcgaatgg ccaagaatct tccacatgtc gacaccgatg cgctttcgca
     2161 gcgcatcacc cggacgccct tccccggatc gcgcaaggtc tatctggacg gatcgagtcc
     2221 ggatatccgc gtcccgtttc gcgaaatcac gctcggcgac accctggtgg acgacggtgc
     2281 cggtggctcg cgccgcgagc ccaatccgcc gctgcgggtc tacgacgcgt cgggcccgta
     2341 caccgacccg caggccgcga tcgacatcac ctgcggcctg ccgccgctgc gtgccgcgtg
     2401 gatcgccgac cgcgccgaca cagtcgcgct gcccggcatc agcagcgcct atggccgcca
     2461 gcggctgcac gacccgaagc tgaccgcgct gcgcatggcc caggcgccgg tgccgcgctg
     2521 cgccaagccc ggcgccaatg tgtcgcagat gcactatgcg cgtcgcggca tcgtcactcc
     2581 cgagatgcat tacatcgcgg ttcgcgagaa cttggtcaaa gcacagttgc gcgagcgcct
     2641 ggcgaccgag cgtgtgccga agcgaggcca ttcattcggt gcaaatctgc aggacatcac
     2701 cccggaattc gtgcgcgacg aggtggcgcg gggccgcgcg gtcatcccga gcaacatcaa
     2761 tcatccggaa agtgagccca tgatcatcgg acgcaacttc ctggtgaaag tgaacgctaa
     2821 catcggaaat tcggctgtca cctccagcat cgaggaagaa gtcgacaagc tggtctggtc
     2881 gatccgctgg ggtgccgaca cggtgatgga cctgtcgacc ggcgagaaca tccacgagac
     2941 gcgcgagtgg atcctgcgca actccccggt gccgatcggc acggtgccga tctaccaggc
     3001 gctggaaaag gtgcacggca aggccgaaga cctgacctgg gagttgttcc gcgacacgct
     3061 gatcgagcag gccgagcagg gcgtcgacta cttcaccatc cacgccggcg tccggctggc
     3121 ctacatcccg ctgacggccg aacggctcac cggcatcgtg tcgcgcggcg gctcgatcat
     3181 ggccaagtgg tgcctgtctc accaccgcga gagcttcctg tacgagcact tcgacgagat
     3241 ctgcgaaatc atgaaggcgt acgacgtgtg cttctcgctc ggcgacggac ttcgccccgg
     3301 ctcgattgca gacgccaacg acgaggcgca gttcgccgaa ctgcatacgc tgggcgaact
     3361 gacccaggtc gcctggaagc acgacgtgca ggtgatgatc gaaggccccg gccacgtgcc
     3421 gctgcagctg atcaaggaga acgtcgacaa gcagctcgag gcctgctacg aggcgccgtt
     3481 ctacacattg ggtccgctga tcaccgatat ttctcccggc tatgaccaca tctcgtccgc
     3541 gatgggcgcg gccaacatcg gctggtatgg caccgcgatg ctgtgctacg tgacaccgaa
     3601 ggagcatttg ggattgccga accgcgatga cgtcaagcag ggcctgatcg cctacaagat
     3661 cgccgctcac gcgggcgatc tggcaaaagg gttcgccggc gcgcagatgt gggacaacgc
     3721 ggtgtcgaag gcgcggttcg agttccgatg ggaagatcag tttcgcttgg ccatcgaccc
     3781 cgacaccgcg atggcgtacc acgacgagac gttaccgaag gaaaacgcca aggtggcgca
     3841 cttctgctcg atgtgcggtc cgaagttctg ctcgatgaag atttcccagg aggtgcgcga
     3901 tttcgcgcga ttgcagaacg ttgaggcctc ggtttcggcg gtgattccga tccagacggc
     3961 gctggaggaa aaggctatgc agtttcgcga gggcggggga gaaatctacg cctgacccgg
     4021 tgcgcggggt ttcagaagat tgagctggcc tgggacggac tacaaatgct ttcccctgtc
     4081 aggctggccc cacagccggt tcgacccccc cacctgagga gtaccgctgg cgctttttgt
     4141 tggtgacaat gtttcgtcag tagtgaaagt ccgtattccg aggaaggcgt gcggggtgtg
     4201 tgtgaggggg caggcgctgc cttctcccgt gaccaatgac tcatgtggac tcaacacgtg
     4261 agccacgaat cgacccctga cacattcggg aacgtactat cgcggtaggt tcaatgcgcc
     4321 gccggatggg tatctgcaac ggcttgacga attgaggatg gacgtggaca aaatgcacca
     4381 atcgccagaa gaggccccca catcagcgtc gggatcggga tccactgagc ctttgtcggc
     4441 cagcgcgctg gcgcggcgtc gcgcattgtt caagggaatc ggcaaaggcg gcgcggtact
     4501 tgcggcatcg gtgcctatcc agaccttagc cggtcagacg ttgctgactg ccggtggcca
     4561 gcatatttgc agtgtttcgg gcatgcagtc aggtgtgcat tcagcgacta ccaatgtggc
     4621 ttattgcggt ggttacagtc caggctggtg gggacagagt acggaccagg gcggcttgat
     4681 tccgaagagg tggcctccaa cgcttgagca cggttggacc tacaaaacgc tgtgcaaaga
     4741 cgttttctcg ggctccagtg tgcgaaacga tgacggctcg ttcccgtcgc tgtttcaggt
     4801 tatgtccccc aaggacaaga gcggggattt ttccaattcg gacgagttcc actgggtgtg
     4861 tgcttggctg aacgctataa cgcacagctt caatttcccg tacaacggcg accaagtcat
     4921 tgccttctgg aatgctggcg cgaccagcga tacctataaa gatgcactga gctttttcaa
     4981 gacatacatg gagcagcata cgtcttgatg ggctccggcc cgtgttccaa ttgagcgatg
     5041 agatggcgta cccgttcgct tgcatcgctt cactggcgtt gctgggaatc gcagtgggtc
     5101 gttttcaacg tcgggtcggg ccaaacgcat ctgcttgacg cggtcaccgc ggccgttctg
     5161 tcaaccattg aatcgtcacc aggcaacgcc gatgacttga gcgccctcga actgcaggtt
     5221 gcgacgcttg gcgagcgcat ccttccgtca gacaccctca aatctgtgct tgagcaattg
     5281 gtcggtgccg gactgatcga agcggcgaca tcttgaatct gtgctcgttg tcgccggccg
     5341 aagtttcgcg ccgactgtgt ggcgaaggtt tgttgctgcg taccgggcca ttcaactttc
     5401 gaatcgtctc tgcgatcgaa tcagtcgctg cagggctgag attgctctac gcggactacc
     5461 cgctcgccgc cgacgacgat ttcgtcgact tcaccgtgac gctgcagtgc agcggcggtt
     5521 tgcggcgatg gtggcggcgg caagtcatcc tcggctacga tggtgcgaac cctttcgtcc
     5581 ccctgccgct ggatcacgcc ttccccttgc tcgaatgggc catgaactgg tgcatttcga
     5641 cgcaggccca ccatgtactg accttgcatg cagccgtgct ggaaagaggt ggccgcgcga
     5701 tgatcctgcc cgctccaccc ggttcgggga aaagcacctt gtgtgctggc ttgaccagtc
     5761 gcgactggcg cttgctttcc gacgagttga cgttgctttc gctgtctgat gggtcggtgg
     5821 tgcctttggg gcggccgatc agcttgaaga acgagtcatt ggcggtaatc cgccaattcg
     5881 cgccggatgt ggtgctgaac gacgtcacgc acgacacgtc caaggggtcg gtcagccaca
     5941 tgaaagtccc ctcagatcag gtgcggaggc tggacgaggg cgctaggccg cgttgggtgg
     6001 tgttcccgaa gtacgtgcct gatgctcctc ccacgttgcg gcctcgctca aaggccgaaa
     6061 gcatgctcga actgggccgt aacgccttca attacacatt gctgggattg accggattcg
     6121 aggtgctggc cgatgtggtg gacggctgtg attgctatga cttcagctac agccgactgg
     6181 aagatgcagt tgctgtattc gatcggctgg tggtggagac gccaccgtga tgtctgacct
     6241 cttgtcgcca gtctgggccc agcccgaact gcggcccgag ttcagcctcg cacaatggga
     6301 ggccgtgctg ggccagtcac gacaggccgg gctgatgggt cggctggctc ggcaccacgt
     6361 cgatcacggc tggatgaccg aagtacctgc caagccgcgc ctttatctcg aaggtgctct
     6421 gcgacaggtc gatcgactgc atcatgagtt gcgctgggag atcgactgca tccaccgggc
     6481 cttggcgccg gtcggcgtgc cattcgtgct gctcaagggc gcggcctatt tcgccgctga
     6541 cttgccgccg aagcgggggc ggctgttcgg tgacatcgac atcatggtca ggcgggagca
     6601 cctgcagcag gtcgagagcg cgctttttcg agccgggtgg atcagcgatg agcgcgacgc
     6661 ctacaaccag cgctactacc gcgaatggat gcacgaaatc ccgccgatgc gccacgtcca
     6721 gcgcggcacc gtgatcgacg ttcatcacac gatcacaccg ccgacctcgc aaatgtgcgt
     6781 tgagggtgag gccctgctgg agcacattgt ggccgtcgat gcactccagg atgtctacgt
     6841 cctcgcgccg gtcgacatgg tgctgcacag cgcagtgcat ctgtttcagg aaggagaatt
     6901 cgagcgcgga ctgcgcgacc tgctcgacct gaatgacctg gttcaccatt tcgagtcgca
     6961 cgacccagac ttctggcgcc agctgatctc ccgggctcat gccctcaagc tgcagacgcc
     7021 gttgcaccat gcgctgttcc acctgaagcg cctgttcggt acccacgtac cgcctgcctt
     7081 tcagggcgcg gtcgagttgc ttcggccgcc ttggtggcag cgtcgcctga tggccatgct
     7141 gctggaggcc gcgctgcggc cggttcaccc gagctgtcgt cagacgggcg aaggtctggc
     7201 ccgctggctg ttgtacgtgc gttcgcatgc tttgcgcatg cccatgaccc tgctcgtgcc
     7261 ccacctcgtt cgtaaggcct ggatgcagcg ctttccgacg aagaagcccg aggaccaccc
     7321 agccgccgag gcgtgagggc ttttccgggt cggcgtctac tgcttcggct tgtcggctac
     7381 cggcgcgata gcgacgaagc caatgcgtgt gagtccgccc tgctgcacga tgcctatgag
     7441 ttcggctact cggccgtagg gcaccgcctg atcggcgcgc agctgcactt cggtctgcgg
     7501 gttcaggcgc gctgtctcgg ccacccgctg cgccagggcg gctgcggtaa tttcgctgtc
     7561 agccaggaac aactggccac ccgaatcgat ggacaccgtg atgaactccg gtgattcgct
     7621 gggctgcgca gcttcggtct tgggcaggtc gagcttcagg ctgctgctca tcagcggcgc
     7681 cgtgatcatg aaaatgacaa gcaggaccag catcacgtcg atcagaggcg tcatgttgat
     7741 gtcgctcatc ggctgggggc ccgtggtgcg ctcgaggcgt ccaaaagcca tggcgtctcc
     7801 tcagctcccc tggggggttg acgggtcgga ggtgcggtcg acctgcatct cgcgcaggtc
     7861 atgggcaaaa ccttccagct cggcctcgca ggtgcccacg agcttgccaa agatgttgta
     7921 ggccagcacg gccggaatcg ccaccgccaa cccggccgca gtcatgacca gcgcttcacc
     7981 gactggtccg gagatgcgtt cgatggtgat cgtgccggcc gacgagatgc tgaccagtgc
     8041 gtggtagatg ccccagaccg ttccgaacag accgatgaag ggagccgtgc tgccaatcga
     8101 ggccagcagc acctgcccga actgcaactg cgtcagcacc gcatgcaatg catcgcgcag
     8161 gcggcgggtc agttgcgatg cgcgctgtcc atgcgcctcg agagtctgcg agttcggttg
     8221 tgccgtggcc gcggtcagca acggtgtcag cacccgctcg cgatcgaaag ccgcaagctg
     8281 gtctcgtgcg gcctccaggt cggcggccgc ccagaatgcc ggaacggccc gcgcgatgtc
     8341 gctgcgcacc cggcgcagca gccagccctt ccagaagatc agcacccatg cgctgatcga
     8401 catcaccagc agcaacgctg cgacgctgcg cgtgatcgcg tcgccttgct gccagaactg
     8461 ctgaaatccg ttcatttctt gacgcgtgga gaagtgcgac ggcgctgtgt caggacaggc
     8521 cgagcacgtc gttcatgtcg aaaagaccgc tctcgcggct ggccagaaag cgcgctgccc
     8581 ggaggctgcc ctgcgcatag gtagcgcggc ttgagctctt gtgggtgatt tcgatgcgct
     8641 cgccgatgcc ggcgaaaagc accgtgtggt cgccgaccac gtctccaccg cgtatggttg
     8701 cgaagccaat cgtcgatgga tcgcgctcgc cagtgacacc ttcacgaccg tacaccgcgc
     8761 agtccttgag gttgcgcccc agggccgttg cgaccacttc gcccattttc agcgccgtgc
     8821 cgctgggggc atcgaccttg tgccggtggt gtgcttcgat gatctcgatg tcatagcctt
     8881 cgctgagcat ccgagccgcc gtgtccagca gtttcatgac gacgttgacg ccgacgctca
     8941 tgttcggagc cagcgtgatg gcaatgtggc gggcgtggtc cgcgatctcg gccttctgcg
     9001 cgtcggcgaa gccggtggtt ccgatcacca ccttggtgcc cagctcgcga caaatcgcca
     9061 gatgtgccag cgtgccttcc ggacgcgtga agtcgatgag cacctgcgac cgggcaagcc
     9121 ccgcatgcag gtccgaggtg atgatgacgc cactgttgtg accggcaaat gcggtggcat
     9181 cctgacccag agccggactg ccgggcatgt ccagcgcgcc gctcagcgac aggtcatcag
     9241 ccgcgatcac ggcttctatc agcatgcggc ccatgcgacc agaagcgcca gctatcgcga
     9301 tgcgcaatgg ttctgctgcg cccaacggaa agctcatcag ttcgactcga gcggtggata
     9361 gacccggtcc ggggcaacga ccggggcttc gacttgtgcc gccttgggag gcggcttcag
     9421 cgactggatc tgctccgggg tcaatgccag cgacggagcc ttgcccgagg atttgttgct
     9481 gtcgatcgac gcgacaaagg ctatttcagc aggcagatcg ccaccggtgt cgagactctt
     9541 cagtcgctct ccctcgaaga gagccacgat gctgcgccgc tgtggctcgg tgccctggcg
     9601 acggatcgtg aacacgtagt cccagcggtc ggcgtggaac acgtcagcca gcaacgggga
     9661 ccccagaatg tcccgaactt gggctcggga catgccgatc tggactcgcg caacctgctc
     9721 cttggtcacc acattaccct gaacgatatc gaccctgtag ggcgtgacga cactcaagat
     9781 ggtgcccgat gggtccagcg aagagcaacc gctggagcac gcggctgcca acgccagcga
     9841 aacgatgtgg acaggcgagc gcgcgagggg caacaaagac atgacggcat ccagggcacg
     9901 cgagaaaacg gcccgactcg atatgatcga ttcattctag cggccgggct tgtgcccggc
     9961 tcgactggcg ggagccctca tgacgaacgc agacgaactg aaaaacagcg gcttgaaggc
    10021 gacgctgcca agaatcaaga ttctggaagt atttcagcaa acccggcagc gccacatgac
    10081 ggcggaagac gtttacaagg ttctgctcgc ggaaggctcg gatgtgggtc tggccacggt
    10141 ctatcgcgtg ttgatgcagt ttgagcaggc cgggatcctg tcccgcaatc atttcgagtc
    10201 cggcaagtcg gtttttgaac tcaatgaggg caatcaccac gatcacttgg tatgcatgga
    10261 ctgcggacgc gtcgaggagt ttttcgacgc cgagatcgag aagcggcagc gttccgttgc
    10321 gcagacccgg ggattcgaat tgcaggagca cgcgctctcc ctgtacgtcg tgtgcaccaa
    10381 gaaggcgtgc ccgcaccgcg cgagctgatc gccgccacgg cacgcagcat cgtgccagcc
    10441 cgccgaggct cagctcccgc cgcgttccag agcgcgctgg tgccgctcga tgaattcctt
    10501 gtaagtgtcg atgccgcgca gttgcaggat cgtgttgcgt actgctgctt cgaccagcac
    10561 cgccaggttc cgaccggcat ccacggcgat caccgccttg cgcacgggca cgtcgaggat
    10621 gttctcgtac agtggttcgt atgggagtcg ctcgaactcg cgctccatcg tttccttgcg
    10681 caccaggtga acgatcagtt tcacgcgcat cttgcggcgt acggctgtct ctccgaagat
    10741 cgccttgatg tcgagcagac cgatgccgcg cacctcgaga aggttgatca acagctccgg
    10801 acagcggcct tccagtgcgg tctgtgacat gcggtagatg tccaccgcat cgtcagccac
    10861 cagaccatgg ccgcgcgaga tcaactccag gcccagctcg ctcttgccga gtcccgattc
    10921 cccggtgagc agcacaccga gtccgaggat gtccatgaac acgccgtgac gcgtcgtgcg
    10981 ctctgcgaac agctggctca ggtagccgcg aaccatgtcg atcacctgac cagccgactc
    11041 tgcagtgaca aacagcggaa tggaagctcg ttcgcacatg gcgaccagtc ggtcaggcgg
    11101 cacctgatcg tcggcgacga tgaccactgg tggctccagt gtgacgatgc gctggatgcg
    11161 ccgttcctgg tcttccgcgg aagcgtccag aagataggcc acctcgcggc gacctacaag
    11221 ctgtacccgg taggggtgga tgtagttcag gtaaccgacc agatcggctg ccgactgcgc
    11281 attgcggacg gctgcttcgt cgaaccgacg ttctggatga gcgtgaccgg caatccattc
    11341 ccaacgcagt gcaacgcggt ggtgctcgaa cagtgcctct gcgctgatgc tgatgggctt
    11401 catgcgttac atgcgcgcaa tgaaggcttt gccggcacga gcgggcgtgc gctccgcctt
    11461 ggcatcaggc caccgacttg agaggttccc agttggcaat gagttcgtgc accttggcgg
    11521 cgtcgacctc tgttttcagg cgctcgcgca actcgcggtc gctgagcatc tccgcgatct
    11581 cggacaggat ttcgaggtga cgttgtgtgg ccgcttcggg caccagcaga aagatgagca
    11641 gcgtgacggg ttcgtcgtcg ggggcgtcga agggaatcgg ctgctgcaca cgtgccacag
    11701 cagcgagcgg gttcttcagg ccctttattc ggccatgcgg aattgccacg ccatgaccta
    11761 gcccggtcga gcccagccgt tcgcgcgcga acaggttgtc ggtgaccgtg gcacgagcga
    11821 tcgcgtgctg gttctcgaac aacaggccgg cgtgttcgaa gacgcgcttc ttgctggtgg
    11881 catccacatc cacgagcaca ttgctgacgg gcaggattgc ggcgagacga ttcatcgggc
    11941 gatccaagtg acaacttcag cgggagcttc gttgcgcgct tgcgtggagc caaaagcgct
    12001 tgattataga aatcggcagc tgttaccgca ccgacggtgt tccatatgca acaggattgt
    12061 gcacacgatg ttgcgatgca gcaatgccat cggccccccg cgcttgcggg cccgttgcgc
    12121 gcggacaaaa aagcggcccg aaggccgctt ccgtgattcg accacgagcc tgcattcagg
    12181 ctgcgcgagc gtcgaggcgt ttcgctgcca catggtggtg gtcctggagc cgtcccttgt
    12241 gccggcacac ctgacgatca agcttgtcca tcaattgatc aacggcggcg tacatgtcct
    12301 cggctgcatg ctcgatgaag atgtccttgc ctttgacgtg gagcgtgact tcggccttct
    12361 gccggcgctc cttctcggtc atcttttcta ccgacagcag cacgttgatg tcgaccacct
    12421 ggtcgaagtg acgggtgaca cggtctagct ttgtgagcac atactcacgc aaagaggggg
    12481 tgatttccaa gtggtggccg ctgatcgtca agttcatggg aactcctttc gcagggatga
    12541 aagacaggga acacatcggc tggccgcctt gcgatggacg gcatgggcaa cccgaccgaa
    12601 atttcatctt gctcctgatc cgtcgcactg gcaaggattc gggccattcg ctcagctttc
    12661 attgcgtgcg gtctgcccgg tttggtggcc ttggtttcat gttgcgatgc gataattttt
    12721 gcgtgttgaa tcagttggag ttcgacttgg acatacccgc cccgcggtga ccgtccactc
    12781 cgcgcagacc tcgcgatgca ccgcgaggtc gggccagggg tgagacggtt gcgatttccg
    12841 caccccttta ggcccccgac ggctgcaagc gcctccgtcg gcttgttgga gctgccatgc
    12901 tcaatgtgtt ctcgctggcc aatggccgcc tcttccagga agagatcgaa agccgcgccg
    12961 cgctgtcgaa cgtgcagccg gtctgggtcg atctcgatgc acccactgcc gaggaaaaag
    13021 gctggatcgc ccagcacttc ggcgtcacca ttcccgacga tgcggtcgat gacgacctgg
    13081 aggaatccgc gcgcttctac gaggaagaca acggcgaact gcacatccga agcgacttcc
    13141 tgatcgacga tgacgagacg ccgcgcaacg tgcgagtcgc cttcatcctg ttcaagaacg
    13201 tgctgttctc ggtgcatgga gaggagttgc cggtattccg gctgcttcgg ctgcgcgcgc
    13261 gacgcatccc cgcgctgatc gaggacgcca aggacgtgct gctgaagctc tacgacgccg
    13321 acgccgaata cagcgccgat gcgctcgaag gcatctacga tgcgctcgaa aaggtcagcc
    13381 agcgagtctt gaaggaagac gtcaacgatg cgatggctgc agaggctttg tcggccatcg
    13441 cgaaagagga agacctgaac ggacgcatcc ggcgcaacgt gatggacaca cggcgcgccg
    13501 tcagcttcat gatgcgcagc cgactgctca atgccgagca gttcgaggag gctcgccaga
    13561 tcatgcgcga catcgactcg ctcgacagcc acaccacctt tctgttcgac aagatcaact
    13621 tcctgatgaa tgccaccgtc ggcttcatca acatcaacca gaacaagatc atcaagatgt
    13681 tctcggtggc cagcgttgcc ctgctcccac cgacgctgat cgccagcgtc tacggcatga
    13741 acttcaacgc catgccagag ctgtcgtggc gctatggcta tccgttcgcg ctggtgctga
    13801 tggtgctttc ggtcgcagcc cctttcatct acttccggcg caagggctgg ctgtgatgcg
    13861 atgaacgaca agtggttgct cgtgctcggt ggactgtgcg tggtcctggc cgtgtttggt
    13921 ctgaaggtct ggcacgccca cggtgtgtgt gacgagatga gcggccgctg gcttgacggc
    13981 gagtgccact tcgatgagcg gtccgcagac gtgaagcttc gctagtgagt tcaccggcgg
    14041 gcgaggcccc gggcctcgca cttcaagccc ggttgatcgc gctgttgcgg gtggcctgtg
    14101 ccaccacgct gatggtgctt tcgttctcga tgctgcagcc cgtgctggcg gtgcgactgc
    14161 agacgtcggg ggtcagcgcc tcggcgatcg gcgtgctcgc gatgctgccg ttcctgacgg
    14221 tcgcgctgat ggtgccgctg atgccggcgg tcttcgaccg gatcggcgtg gctgccgcct
    14281 accgctgggg catgggcctg gaggccctcg cgacgctggg ttacgcagtc agcgagcact
    14341 acgccctgtg gtgcgctctg gcggtactgg gaggcatcgg tgccgcagcg gtctggaacg
    14401 gcacggaggc tttgatcgcg cacaacgcgc ctcccgagcg acggggccgt ttcaccggtc
    14461 tctaccagag cgcactcggc gcagccctcg cgatcggtcc cttcctgccg ggcctgctgc
    14521 cgttgtcacc gcgcgcgctc acggtgcttg cgtctgcact gatggtggcg ggactgttca
    14581 ccaccttcag cagggaagtg agccgcctgc aggctggtca cgaagatgcg ctgcccgccg
    14641 gtctgtgggc gacgatctgc agtgtgccgg gactggtggc gatcgctttc gcgggcggag
    14701 tgttcgaggc cggactcggc tcgatcacga cggcctacgg ctcgcaggcg ggcatgtcac
    14761 tcgccgcggc cgcgtcggtg gccggtgtcc tcggtctggg aagttttctg ctgcagtacc
    14821 cctgcggctg gttgtcggat catgtttcgc cgcgccgcgt tttcgggctg tccggggcct
    14881 tgctgctcgc tgcgtcgggg gccttcgtcg cggctgcggc gtggccaccc gtgctctgga
    14941 tctgcgcttt tgtctggggc gctgtcggcg gggcgttgta cacgctgacg atgatccgcg
    15001 tggcgcatca gtttgctgcg tcttcggctg tcgccggcac tgctgccatg atcacgggct
    15061 acaccgttgg cggtgcggtg ggcccgtcct tcagcggcgt gatgctcgat ttcttcggag
    15121 cgacggggca ggccacctgg ttgagcctgc tggcgatcgc ggtgctgctg ctgtcacgcc
    15181 ggttctccgg cttcgccggt cacggctgaa ggcacgcgcc ggtccagcgg tacgccttcc
    15241 aggaccatcc gctgcgcgag ggcgcgcccg ctgagccagg cgcgttcgat gccatcgtcc
    15301 tgcagcgact gcgaatcggt ggcgccgtgc caggcgtcgc cacacgcccc cagacgcaac
    15361 accggattcc agccgaacgg ttcgtggcgt ggcgaaggaa cccgcgcatg gcgccagcga
    15421 tgcgcggtcg agaactgcgg aactgcgtga tgcacgccgc agtgctgccc gaaggcggcc
    15481 agcagcgccg ccttcacatc ggcaggcacc gcctccaggt gctctcgggt ccaggctggt
    15541 gtggcgtgca gaacccagcg gcagccgatg ccagcgacct cggtgcggcc cgtgcggtcg
    15601 tcgtcgcgcc ttgccaggga cagggtgccg cgtggatcgg cgcatcgata caccgcctcg
    15661 gagaccggcc atggggcgga ccacgctgcc atcaccgtcc agcacgggtc gcttggggtc
    15721 tggcgcatcg catcggatag cgccgcatcc agattcagca agacggcagc ttgttcggcc
    15781 gggacggcga ccacgacggc gtcgaagcgg gccggctcga ggtcgccgtt cagcagccgc
    15841 agacgccagc caccatggcc atcagccgcc tcggcgtcgc cgggtgcggg ctcgatggct
    15901 gcgatctggg tgtcgggtcg ccagttcgct ccctctggca aatgggctgc aagctgtcgt
    15961 gcaagcgaat tcatcgacgg cacaccgaaa gcgtacgagg gcgctgctgg atcgccgctc
    16021 acctcgcccg aatcgccttc aatggccacc cacccgctgt ctcgccagcg cgagacctgt
    16081 gctcgaaatg aatctgtcgt tgccgtgaat ccgggtgccc catggtcgaa ggcgccggcc
    16141 tcgctgcgac gggtcgcgca acgcccacca gccccgcggc tcttctcgaa cagcgtcacc
    16201 gaccagccgc ctgccagcag tccgctcgca gcggtgaggc cggcgatgcc cgcgccgatc
    16261 acggcgacgc gcttcatcgg ggtgctcgcg ctcacgaatt cgcccgccgc ttcagccagc
    16321 gcatcagcgc gccgacagcg ggcagtttct cgtacagctc ttccgccaca tcccagtagt
    16381 cgcggtgcag cgtgatcagc ccctctgcat cgagcttcaa gtgcgaggcg ccgcgcacgg
    16441 tctgcaagtc gctgctgaag cgccggaacc ggaacaggaa gtcccaggtc agcacgcact
    16501 gatcgccctg cacgatcacg tcgtggatca cgaagcgcgg tgcctcgagg gccacgtaca
    16561 tgtgggtgaa gacctgctgg atcgccggca ccccgcgcac ctcgttgaac gggtccttga
    16621 atgccgcatc catcgcatag aagtcggcaa gccgcgacac atcggcgggc gtcagcgtct
    16681 cgaaggcctc gatgatgcgt gcgacgcgcg gatcggtgct gcgcgcgatg ggtccgggct
    16741 tcatcggaaa cgcctacaac ccggtgaagc gccggatcac gcggaagtac aggccgtcgc
    16801 cgacgtggcg cagggccttc aggacgcgag tgaaccgctt cgggaagtgg atctcgaagc
    16861 gtcccgctgc ccaacccgcc agcatctcgc gcgcagcctc ctcgggctgg ataagtgccg
    16921 gcatcttgaa ctcgttgcgc gcggtcagtg gcgtcgcgac gaagcccggg cagatcaccg
    16981 acaccccgat gcccagcgga tgcagatcga ggtagagcgt ctgcgcgagg ttgatcagtg
    17041 ccgccttcgt cgggccatag gccagcgagt tcggcaggcc gcggtacccc gcgacgctcg
    17101 acaccaggct cagatgcccc gacttctggc ggatcagcac cggcagcacc gcgtccagca
    17161 ggtagagcgc gccgacatag ttgaccctct cgtgccgcag catttcgtcg aggtcgtaac
    17221 tgctggcgcg catcggcgcg tagtggccag cgcagtacag cgcgaaatca atgcggccgc
    17281 agcgggcgac gatgcgctcg gcggcctgct tcatcgcatc ccggtcggtg gcgtcgaggg
    17341 cgattcccag gctgcctgga tggctggact cgaaggcttc gaccgcagcc gcattgcgtg
    17401 ccgagacgac gacggtggct ccgcggtcgt gcagttgccg ggcagtcgcc aggccgatgc
    17461 cgctggaggc gccgaccagc cagaccacgc gtccggtcca ttcgttgatg cggggattca
    17521 gcgacatggc atcgcaccga ccgaccggga tgccgactgc ccctcaggac gcgacgggac
    17581 tggccggagg cagtccggcg cagcgcccca cgaagattga tccgcctgaa ccggtctcat
    17641 ggcttgtgga atgccagagt gacttcaccc agacgtacgc cgaacttgct catcaccgcc
    17701 ttgttcagca tcacacgctc gtccaccagg tacatccagt catcgaactg cacctcgtag
    17761 gtcttggcat cgaccggcag tcgcagcgtg tagctccact ggaaggcatt gcccgagctg
    17821 cgtccctgtg ccgtgccgac cacgtcgtcg gcggtgccgg tgtactggcc accgggcagc
    17881 ttcttcagcc gccacacgcg ctgctgcttc tcgccgtcgc tgtagacgaa gcgctcgtcg
    17941 agcacgccgt cgtcgccctg ccagctgcag gccatttcca ccgtgaacct gcgcacgacc
    18001 ttgccagagc ggtcggtgaa gatgccgtgc gcggtgaggt ttccgttgaa gtaggccttc
    18061 aggtcgagta ccggcttctc gttcgcgtag tcggcggggc tcggcgatgc gcaggcggcc
    18121 agcaaggcgg tggcagcggc tgtcgcagcc aggatcgaac gtcgattcat tgcggttccc
    18181 gaaggttgcg tatcagaagg gtgtgcagca gcgcggccgc ggccagcttg agcaggcagg
    18241 gcagcatgca gtaggcggcg gtcagcgccg ccagcgcctg ggggctgcgg ctgcccggtt
    18301 cgtacccgaa ccaggccagg gcgggcaatg cgaccccggc agccagcgcg agattgagct
    18361 tggtcgcgaa attccaccag ccgaagtagg ctccttcggc ctgttccccg tgccccgcgc
    18421 gctggatcac gcctgcgagg atcgcgctcg gcagcgtcag gtctgcgccc agtgcgacgc
    18481 cgctgagtgc gcacacggcg gtgtaagcca gcacgtcgcc ggcgcccagc aatgtcgccc
    18541 agctgaacac agcgatcgtc agccccatgc ccagcagcca ggcgcgctcc aggccgaccc
    18601 agcgcaccgc acgcacccac agtggaatcg acagcgcgcc ggcggcaaag tagctggcga
    18661 gaaagagcgg ctcgtaggcc ggggcctgca gtcgatcgcg gatgaaaaac agcaccagcg
    18721 ttgccggcac ggcgctggcg atcccgttga cgagatacac cgccagcagg cggcggaagg
    18781 cgggcgtggc aaagggcagg agcatcggca agtgggctgc aaccaccgcc gatgcggccg
    18841 gttgcggcgc ggcacgcagg cacagcaccg cgaagaacag caatgcggcc agcacgccga
    18901 ccgtcaccgc cagacccgcg accgacggca gcacgcttgc gaccagcacg ccagccagtg
    18961 ccagaccctc gcgccaggcc acgatgcgag cccgttgagg cgcgtcgccg cccagtctgg
    19021 ccccccacga ctgatggatg acggagactc cgctgtaacc gaggtaggtc accgcgagcg
    19081 ccagcgcgca ccagaccagc agtgcggagc ggtcctcgac cggaggaaag aacagtgcgg
    19141 cgaatccgat ggcgagcacc agggcagcca tcgcgatggc ccaccaggcc cgccgtgcgc
    19201 cgcctgccag cagtcggtcg gctgcgcggc cgatcaacgg gtcggcaact gcatcgaaag
    19261 cgcgcgcgcc cagcagcacc gcgcccagcg tggccagcgg gatgccgaac tcgcgtgcgt
    19321 aatggtcggg cagcacgatg tagagcggca gcgcgacgaa ggccaacggc agaccgagtg
    19381 cgccatagcg cacgcccggg cgccagccgg ccagtccagg gccgtcacgc ggtgagccgt
    19441 cgggctcggt tgctgccgtg gtggacgggt gctcggacaa gtcggcggcc gggatgacgt
    19501 gctgcagggc catcgacgac ggcccggtca cgatccggtg cggacgcccg ccagcaacgc
    19561 ctcacgcaac cggggttcgg aggttcgctt cgacagccag atgccgaaga acaggcgtgt
    19621 gaactcggca tcgcggatct ctcccgtcaa tttgccattg acgaagtagc gcgcggcgac
    19681 acccgggacc tgtatgcccg tgacgcggtc gccctcggag acgtcgggga tcgactgttt
    19741 catcgccgcc agccatcggg tcgccttcgc ggcatcgatg gctccgctgc gctccatctc
    19801 ttcgatcgat cgcgcagcga tctcggtgct ttcgaagctg cgtgcgtact cgagctccag
    19861 ggccagtggg gcctggtcga agttgtcggc gtcgaactgc gggccggtcc acagccgggc
    19921 atcgtagacg tggaacagca ggaatttcat ccggccgctg ccatgcagcc gcgctcccgg
    19981 cagctcgctg cgcacttcgg gcggcggtga cagggcggcg cgtgcgacag ccagcacgct
    20041 tgccggcagg cagccgatgg ccagcggcag cgccagcagc gatcgtcggg tgtggtcgag
    20101 cagatccggc tggcgcatgt tagtcgcgct gcagcgtgaa ctgcatgacg ttggtgttgc
    20161 ccgcctcgaa ggctgcctcg cagtacgcga gatagaactc ccagattcgc atgaagcggc
    20221 tgtcgaaacc aagccggcga accggaccct cctgactcag gaagcgttgc cgccagcgca
    20281 gcagtgtttc cgcgtaatcg agcccgaatt ccagctcgtt gaccactcgc aggcctgcgc
    20341 gcgcggcggc cttgcggaac tcggccggac tgggcagcag tccgcccggg aagatgtact
    20401 gctggatgaa gtcggtcgaa ccgacgtagc ggtcgaacag gtcgtcgcgg atgacgatgc
    20461 tctgcacgca cgccttgcca ccctgcttga gctgcgagtt caaggtctcg aagaagctgg
    20521 gccagtactc gcgcccgact gcctcgaaca tctcgatgga ggcgaccgca tcgaatggac
    20581 cggcgctgcc gtcggtcagg tcccggtagt cctggaagcg caggtcgccg ggcagtcctg
    20641 catcggccag gcgcttgcac gcccactcga gctgttcgct cgataacgtc acgccggtga
    20701 ccgtggcccc gaagtcgcgc gcggcgcatt cggccagcga gccccagccg cagccaatct
    20761 cgagcacgcg gtcacccggc ttcacgtcgc attcggtcaa tgctcgccgc accttggccc
    20821 actgcgcttc ttccatcgga cgggtgcggt ctccctcgaa ccaggcgctc gaatagttca
    20881 tcgtcgggtc gagccacagc cggtagaact cgttgccgag gtcgtaatgc gcgtggatgt
    20941 tcttcttgct gccggatcgc gaatttcgat tcagccagtg cctgaagcgg tacagcagcg
    21001 agccccacca ggtgccgtag accagggact cgacctccga tccattggcg agcatcagcc
    21061 ggatcagcat ggccggatcg ggcgaggtcc agtcgccggc gatgtaggtt tcggcgaaac
    21121 cgatgtctcc ggagcgcatc gtcgcaccgc agacgttcca gttcagcagc cgcatcgcag
    21181 cccgcattcc gccgtcgacc gccgagccga aatgcagacc cgtgccatcg ggcagctgga
    21241 tgtccagcgt gccgtgcttc aggttcttca acaggcggaa taccgcgcgg gcggccgctg
    21301 gtgccgacgc cggcagggcg ccaggtgaca gggtggtggt gtgcatcgcg tcgagttggg
    21361 acatgccagc gagagcggga aagtggaagt tcgacgcgcg gtcagcgcgt cacgaaaccg
    21421 atcgggggct cgggcttgcg gaagaacggg acccgcttca tccacagccg agcggcctgc
    21481 agatggatgc gaccgatcac gccgagtgtc atcagtggca tgccaaagaa cgcacgccgc
    21541 aggctgcggc tggtcagcgg cagcagatcg ccggacacgc tggtctggat cagcgcaccc
    21601 tccgcatcgt cgtgatcgat gcgcgcgacc gtgcgcgtca gctgcatcct ttccgatgga
    21661 ccggccatcg gcgctctgct gtgcatgaag cggaaccggt aaagaccggc gaccgagcag
    21721 aacggcgaca cgtggaacac cttggtcgcc acttgctcct tgccgtaggc gagctgcgcg
    21781 ccatgcagca ggtagcagtg ccgctcgcca aaggtgttgt tgacttcgac cacgacagcg
    21841 gccagcgtgt tgtccgcccg gtggcagtac cagaagctga cgggcttgaa ggtgtgcccc
    21901 agcacccgtg ggtagcaatg cagccagacc tcaccgccgg catccgtgat gccctcgctg
    21961 cacagcaact cgtcgagcca ggcgaggctg tcggcacgac cgtcaccatg gtcgctgtcg
    22021 tggaagctga tcaatccgaa gcggttgcgc gccagatcgg cgtgtggcgc gtcgcgcagg
    22081 cggcgcatcg gaagcatcag gaagtaggtg ggataagcga agcggttgac ggccggtcga
    22141 agccgggtgt ggcgcacctg accgaatccg atcattggac cggaacgctg cgtcgagggc
    22201 gaggctgcgg cactcatgcg gccaccggca ccgggcgggt ggccgtctcg gcccagcgct
    22261 tgcgcaaacc atccacgacg ccgatccccg acatcagacc gtcctcgtga aagccgtaac
    22321 gcgtccaggc gccacagaac caggtgttcc ccacgccctg aagcaacggc acccgctgct
    22381 gcgcagcgat cgcgtcggcg tcgaaaaccg ggtgggcata gtcgtacacc ccgtggacgg
    22441 tgtcggcgcg cggctcgttg acaggattga gggagaccac cacgggggtc ttgaacggca
    22501 gtggctgcag ccggttgatc aggtagtgca ggcagaccgc cgtcgcttcg cgcgaatcgg
    22561 ctcgggcgcg ttcatagttc cacgccgccc aggcgatctt ccgactcggc agcaccgaag
    22621 tgtcggtgtg cagcaccgcg cgattgcggt ggtaacggat ggagcccagc acttcacgct
    22681 cgtcgggcgt ggcatcggcc agcagcgcca gcgactgatc gctgtggcag gccagcacga
    22741 cgtcgtcgaa cgactcgctt cctctgtcgg tgccaatgct cacctggcct gtggcgcgca
    22801 tccgacgcac gctgcgcacc ggcgtgttca gccgggcatc ggggacgcgg ctgacgagct
    22861 tctccacgta gtggcgcgca ccgccgcgca cggtgtacca gcgcggccgg ttcgtcacct
    22921 ggatcaggcc gtggttgtga cagaaccgga tcatcgtggc gatcggaaag cgcagcatct
    22981 gatcggtcgg acaggaccag atgcagccga tcatcggcag gaaataccaa tcccggaatt
    23041 ccgcgctgaa gcgatgctcg gccaggaatt cgccaatggg ctgctgctgc tcggcttctt
    23101 ccccgcgcac ggccaacgtg gtggccagac gattgaagcg tgtcagatcg cgcagcatgc
    23161 gccagaaagc cggtcggagc aggttggcgc gctgcgcgaa gacgctgttg aggtcgctcc
    23221 cgctccactc caggccgatg tccggcacct gcaccgagaa cgacatatca gagagcgcgg
    23281 tgggaacctg cagctcgccg aacagcctga tcaggttcgg gtaggtgcgc tcgttgaaca
    23341 cgagaaatcc ggtgtcgact ccgtgcgtga tcccgtcaag ggtcacatcg acggtgtggg
    23401 tgtggccacc gaagtagctc cccgcctcgt acaaggtgac ccttgcttcg ggcgcgaggg
    23461 catgagccac cgacaagccc gagatgcccg agccgatgac tgcgacgctg cgcacgtgat
    23521 caggcatgtg cgtacggcgc ctgccgaacc gtcggcttga ccgagaagaa agaagctgca
    23581 cagcgcccgg cagggcaaaa cgaagggagg aggcaaagaa aataccgcca ccaggattgt
    23641 gtcggcggga ccgacgagct gtgcaacgct aaaccgtggg tcgggtattt ggtccgccgg
    23701 tgtcgcgcag atccggaggt tctgttccgg tcgctggacg cctgactcgc catggcactc
    23761 gaccctgcgt agacatccga tcgaaacgag agttcccctg ggttcattcc tggaagcagc
    23821 cgtcctgtgt cttcgggaat ggacgtacag ctcgcaaatg cttggactct taccatttgg
    23881 ttcaaaatat aactgctcgg tatgggtcgc gcggattgct gccgtatttc actgcgcaac
    23941 cagcagcttt tcaatgcctt cgacaaacgc cttgtcgtcg ggttcgacat cgctgctgaa
    24001 gctctgcacc gtggagccgt cgcgtccgat caggtatttg tggaagttcc agcgaggcag
    24061 ggtgccggtg cgctgccgca actcggcgta cagcgggctc aaagccgctc cggcggactg
    24121 tgacacgcga gtcttggtga acatggggaa cttgaccccg aaggtgtttg cgcagaacgt
    24181 cgcgatctcc tggttgctgc caggctcctg gctgccgaaa tcgttcgacg ggaagcccag
    24241 caccaccagg ccgcggtccc ggtagcgggc atggagcgat tccagtccct tgtactgtga
    24301 ggtgaagccg cagtagctgg cggtattgac gacgacgacg accttgccgg cgtactggca
    24361 caggttctgt ggcttctcgt cctgaagtct cggcaactcg cggttgagca gggtgccgca
    24421 ttccgccgct tgtgcgccgg aagcgcaggc gaataacagc agcgctgccg cacaagattt
    24481 ccttgcaaag tcgatgagct gagcagttga catgtccata tcctgtcttg ggcaaagaat
    24541 tgtcaaagcc gtatattgac gtaaatctaa tcacctgtcc cgcaccatca catgcctgat
    24601 cacgaccaga catcgatcgc cttcgtcgat taccccgagc ccggcaaact cgtggatcgt
    24661 ctgctgcgat gaatgaaccc gcgcgctcgc tgaaatcgct cacgctgtcg atcgccgcgg
    24721 ccgagcgcga caccggcctg accaaggaca ccctgcgcgt gtgggagcgc cgttacgggt
    24781 tcccccggcc cgatcgcgat ggtggtggcg agcgtgccta tccgctcgaa caggtcgaga
    24841 agctgcgcgt cgtcaagcga ctcatggacg cggggcatcg gcctggccgg ctggtgtcga
    24901 tgcctatcga ggaactggtc cggttgagtg agagcaccgg cgatgcgcct cggcgatcgg
    24961 ccggaagcgg cccgctcctc gatctgaagg cctgcctggc cctgatgcgt tcccacgatg
    25021 tcgatgcgct ccgtcgccag ctgctgcaga cgcagatgcg gatggggctc gcgcgcttcg
    25081 tcatcgaagt ggtgatgccc ctgaacacga tggtcggcga cgcctggatg cgcggccaga
    25141 tggaaatctt cgaggaacac gcctacatcg aatgcctgca tgggttgctg cgccaggctc
    25201 tcgccggcct gccggaggct gcaccgctcg accgcccccg cgtgctcctg acgaccctgc
    25261 ccggggagcc ccatggcctg ggcctgttgc tcgcccaggc gcttctggcg ctggaaggtg
    25321 cccgcgtggt ctcgctgggc gtgcagaccc ctgtgtggga catctcgctg gcagccgcag
    25381 cgcaccgcag cgacatcgtc gcgctggggt tcaccgggtg catgaacccc aatcaggtcg
    25441 tcgacggact gagcgaactg cgcagcaagc tgccgccagc ggtcgaactg tgggttggcg
    25501 gctcggcgcc ggtgctgcac cgccggacgg tcgatggagt gatggcgttt tcgtctctcg
    25561 aggacatccg gccacagatc gagcgctggc ggcacggcag cggctgacca aagcaggtgg
    25621 tttccgcctt gctcgaatga ccatcggccg cgcgcccttt caaggtgcgg ctgcgggtca
    25681 acacgtctaa actcgtgctc cctggaaaag gtcgcaccca ccatgtccac catgctctat
    25741 cccgaacttt tcaagcagct cgaagccgtt cgctggagca tggacaagga catcccctgg
    25801 gaccgtttcg atgccagcca gttgagcgac gagcaggcgc agaccatcaa gatgaacgcg
    25861 atcaccgagt gggctgccct gcctgccacc gagatgttcc tgcgagacaa ccgcggcgac
    25921 agcgacttct ccgcgttcat gagcgtgtgg ttcttcgagg agcagaagca ctcgctggtg
    25981 ctgatggagt acctgcgccg cttccgtccc gacctcgtgc ctaccgaaga agagttgcac
    26041 gaggtgcgct tcgagttcga tccggcaccg gcgctcgaga cgctgatgat gcatttctgc
    26101 ggagagatcc gactcaatca ctggtaccgc cgtgctgccg aatggcacgc cgagccggtc
    26161 atcaaggcga tctatgaaac gctggcgcgc gacgaggcgc gtcatggtgg cgcctatttg
    26221 cgctacatga agcgggccct gagccaggtc ggcgacgaag cacgcgttgc ctttgccaag
    26281 gtcggggtgc tgatggccag cgcccggcgc accgcacagg ccttgcatcc gaccaacctg
    26341 catgtgaatg aaagcctgtt cccgcgcgat accgtgcaga gccggctgcc cgatccggtg
    26401 tggctcgaag cctggctcga tcggcagatc tgcttcgacg ctgaatggga aggcaaggtg
    26461 gtcgaccgca tcctgcacaa catgagcctg ctgatggagc gcagcttcgc gaccgtgcag
    26521 gagctcaacc gcttccgcaa ggaagtcgcc gcgtcgatcg ccgcggccgc tcagtccgct
    26581 gctgccttgc ccgcctgagc cactcggcgc agcgtcgccg ccgcctcatg agcgactaca
    26641 ccgacaagct gtgcccgcgc gatgaggtcg aatcgcgtgt cgctgccctg ccgcagccgg
    26701 tggtgttcac caatggggtg ttcgacatcc tgcatcgcgg gcacgtcagc tacctggcgc
    26761 aggcacgtgc cttgggcggc agtctcgtgg tcgggctcaa cagcgatgcg tcagcgcgtg
    26821 gcctgggcaa gggcccgggc cggccgctca acacgcaggt cgatcgcgcc tgtgtgctcg
    26881 ccgcgctcga gagcgtgagc ctggtcatcg gcttcgatga gccgacaccg gtcgagttgc
    26941 taaagcgcgt gcggccgcag tggtacgtga aaggcggcga ctacgacatc gagacgctgg
    27001 ccgagacggc tctggtgcgg agttggggtg gtgatgcgca ggcgttgccg ttcgtcgatg
    27061 gccattcgac gacggagttg gtccgacgca tccgcggctg agcgacgccc gcgccctagc
    27121 cccgcaagca tcggcacgcc gcagggccga aggcggccct atccgtgtgc atcgaccatg
    27181 cccagggcct cgcgcaccgt cgcgacagcg cgcgacacgc cctcgagcag cgcggatgcc
    27241 atcgctccag acagtgcgcc gctgagatgc gcgccgggcg cgacggcgat atcccagcct
    27301 ggcgggtggc tgatcgcgcc agcccagggc atctctgaca gcagcttcgc ggccagcgcc
    27361 gcgagtacca cggcggcaat tcgacgctgc gcgcgcgttc ccgagcacgc caggtgcact
    27421 gccaccacag aaacccctgc gtgcagcacc cccgagagcc cgccgtagtg ccgcaattcc
    27481 ggttgcatcg tcaggcccca ttgagtcagt ggccacgcga tgaaccaggc gattgagcag
    27541 cgggccggaa cgcgtgcgac ggcaccccac gcagccaccg ccaccgcgcc ggcaagattc
    27601 gcagcgaggt gcaggtcgct gaggtggacg aacacggctg tccacgcgcg ccacggttct
    27661 gcccgggcga gcgcgggctg ccaatcgagc gcctgtgccc agtggctggc cgaatccgtg
    27721 cgctcaccgc ccatcaggtg gagggtcgcc gaaaccgcgg ccatcgacag cgcgatcacg
    27781 acccacgtcc acgctgacgc aggcaccgcg cctgtccccg gctgcgtcac cggtcagccg
    27841 aacaccgcat gccacagcgc cagcacggcg gcgcgttgcg cgggcatcgc atccaggggc
    27901 acctgggtcg gctgttcgtc gagtcgtgcc gcgtgctgcg cgcggcggag gtcacggtag
    27961 gcatcggccg cggcgctgcc gacggtcggt ggcaacagcc ccgccgactc ggcgcgttgg
    28021 agcaacgcaa tgttgccgac gttggggatc agctcggggt gtcgaccgct gtgttcgagc
    28081 accagcacct gcacggcgaa ctccacgtcc atcatcccgc ccgggctgta cttgatatcg
    28141 aacatctcgg ccttgaccga gtgggctgcg ccgacttttt cgcgcatcgc ccgcacctcg
    28201 gtacgcaagc cttccgggtc gcgcggcgct gccagcaccg tgcggcgcac ctcgtcgaat
    28261 ggcgccacca gggcagcgtc gccggcgcag acgcgagcgc gcgtcagcgc ctggtgttcc
    28321 caggtccagg cggtgttgct gccacggccg gtctggtact tgtcgaagct ggcgatcgag
    28381 gtcaccagca gtcccgagcc accgttcggc cgcagtgcgg tgtcgatgtc gaacagctcg
    28441 cctgctgcgg tgcgcaatgt cagccagccg atcagcttgc gcacgaatgc ggcgtagacc
    28501 tcgctggcct tgtcggggtc gggctcgccg gcctcgtcgt agaggaaaac cacatcgagg
    28561 tcgctgccgt agccgagttc cttgccaccc agcttgccgt aggcgatgac ggcaatctgc
    28621 ggttgatcgc ggtgccgttg cttcaggtgc ttccaggacc agcgcagcgc gcaggccagc
    28681 gtggcgtctg ccagtgcgga cagatcgtcg gccacctgct cgacgctgat gtggccttcg
    28741 acgtctcgca ccagggtgcg gaacacctcc gagtggtggg cgcggcgcag cgcgtcgagc
    28801 agcgcttcct cgtcgacctg tcccgagcgg ccccaggccg cgtagcgcgc ctccaggtcg
    28861 gcggtgaact cggtcgcatc gaagcggccg tgcagcagtc gctcgtcggc cagttcgtcg
    28921 acgacgcccg ggtgcagcat caggtagcgg atcggccagc gcgccaggcc cagcagccgc
    28981 agcagccggt tctgcacctc gggacgctct gcgagcagcg cgatgtagct ttcccgacgc
    29041 agcagcggct ccagccagtc gatgaagcgc gccgctgcgg cgatcgtgca gcggccgtcc
    29101 ttcactgcgt ctgctgcgcg cagcaccagc ctggccagtc gcgccttgct ctcgtcacgc
    29161 agcaccttga tcttcgggtg atcgcaccaa ggcttgacgc gcgccgccag ttcctccggc
    29221 agcttggcga ggaattcgcc gctgtcgacg ggctgcaggc tcggcacgca tccgcggcag
    29281 gcaacgccgt tgacctgggc tggcgccggc tgaccttcgt gcagaagcgc gtcgaattcc
    29341 gttgccacga actctcgggt ttcgcacagg cggtcgagca gttcgcacgc atcgacggtg
    29401 cagcgcaggc ccaggctgcc cgcaatccag cgcaggtcgg catcggatgt gggcagcagg
    29461 tgcgtctgct ggtcgtcgtg gaactggatt cggtgctcca gtcgccgcag gaagacatac
    29521 gcctcggcca gccgggctgc actggcgggt ttcatcaatc cgcgggccgc cagcttgtcg
    29581 agggccttca gcgtcgagcg cgtacggatc tcggggaact gcccgccgcg cacgaccagc
    29641 agaagctgaa cgatgaactc gatctcgcgg atgccgccgc gcgacagttt cacgtcgttg
    29701 gcgcgctcgg gccgtcctgc ggcacggcgc tgcgcctcat cgcgcacctt gcggtgcagc
    29761 tgacgcaggc cctcgaacac accgtagtcc aggtagcgcc ggtacacgaa ggcgttgacc
    29821 atgctgcgca gtccctggat ctgcgtggtc tcgaggcggg cacgtggagc gaccacgcgg
    29881 ctcttcagcc aggcgaagcg ctcccattcg cggccgtgag cctgcaggta ttcctccagc
    29941 atcggcaggc tgacgaccgg cggtccggag ttgccgttcg ggcgcagtgc gaggtccacg
    30001 cggaagacga agccgtcctc ggtggtttcg ccgatcagcg cgtacaggct gcgcaccacc
    30061 tgcgagaaat actcgtgagc actcagtcgc tgcggacccg gcgtttcgcc gccgtcctcg
    30121 tagacgtaga tcaggtcgat gtcggaggac acgttcagtt cgcgggcgcc cagcttgccc
    30181 atgccgacga tccagaaatc gatgcgctgg ccggccgcat tgagcggcgg accgctgcgc
    30241 gcatcctgct cgaccatggc ctgcgccagc gcgacgtcga gcgtggtctc ggccagctcg
    30301 gtcatcgcca gggtgacgtc ggccatgctc gcgccgcgct cgatgtcgag cacggccagc
    30361 cgctcaagca ccagctggcg cgtcacgcgc agcgcgctcg ccagtgagcg gccgccagcc
    30421 accagctgtt cgatcactgc ggtgatcacc gcggagcgcg gaagaccttc aggcagcagc
    30481 ggcagctcgt ccacataacg gcggcggatg cgttgcacga agcggctgtg gtcggcgcag
    30541 gccgggctgg ctgctgcgcc gacagccggg ctttcggggc gggagttcgc agcgatgtcg
    30601 gaggacaggg aacccatgga tcgaaagggg ctctgtgcta gattgcttcg acgtgtacgg
    30661 aagggttctt caggatgtcg agggcatcgt gcaagccccg caggccccca cgctgattcc
    30721 gctcatccga ccccgccttg cacggcgccc gaacccaccc atgcctgtcc aagaagtcga
    30781 cgtttccgtt ctgtctcctg gcacccgact gacgctggcc cggcttttga ttcgcatatt
    30841 gaagggtgtg ggcctgctgc tgatagctgc ttggagcctg ttgatcctgg cttgggcgac
    30901 tgcgcactgg ttcattctgc cgcacatcga tcagtggcgc ggtcccatcg aacggcaggc
    30961 cacacaactc atcggacaac cggtgcgcat tggcgcggtt cgggtcactt cgagcggcct
    31021 ggtgcctgcc atcgaattga aggacgtgac gctgctcgac gcgcaggcac ggccggcgct
    31081 gcgcctgccg agcgtgctgg cggcgctgtc ggcgcattcg ctgctgtcgc tggagccccg
    31141 ctttgcacaa ctgctgatcg acggggctca cctcgagatg caccgcgatg ccgccggccg
    31201 cctcttcatc gccggtctcg acttcaacgg accggggcgc aacgaccgag cctctgcgaa
    31261 ctggttcttc aggcatcatg agttcgtgat ccgcggcgga tcggtgcgct ggaccgacga
    31321 actccatccg gcgccgccgc tggaactcag cgaagtgcag gtggtggtgc gcaattcact
    31381 gcgccatcac gacgtgcgca tcgatgcgac gccccccgcc cactggggcg atcgattcag
    31441 cgtgaccggg cgtttcacac agccgctgct ggccgagagc gccgactgga agcgctggcg
    31501 cgggacggtt cacgcgacgc tgcccggcgt cgatgtccgc acgctgcgcc agcacgcgac
    31561 gcttccgttc gatctgacgc agggccaggg tgcgctgcgt gcctgggtcg atgtggtcga
    31621 cggcagtgtc gcggccgcaa ccttggatgt cgcgctgcgc gaggtggcgc tgagattgca
    31681 accggggctc gacccgttgg ggttcgccga ggtgaccggc cggctggcgg gtcgccagga
    31741 cgagcgagac acggaactca ccgtccagca gctcgggttc gtgacctcgg atggcgtgcg
    31801 ctggcccgcg agcaacgcgg ccctcaagtg gcgccgtgac ggaagcggcg ccgtcgcagg
    31861 cggcgagctg acagccgagc ggctcgacct cgcgctgctg acgcaggtgg tgcggcgctt
    31921 gccggtgggc gacggcgcgc accggctgct ggccgacttc gatccgcacg ggacggcctc
    31981 ggaattcagc ttgcgctggg acggcgttcc gaacccggtc gccggggctg cagcggcgtc
    32041 ggccgcctcg gctgccgacg ctgtggcctt gccgccgcgc taccagctgc gcgtcaacct
    32101 cagcggcctg tcgctggccg ccgcgcctgc ggccgatgcg caaactgcgg gccggccggg
    32161 cttgcggcag gccgatctcc gggtgagcgc cgatcagagc ggcggcgagg tgcgcatttc
    32221 gatgacgaag ggtgaaatcg acctgcccgg cgtgctcgag gagccgacgt tgcctttcga
    32281 ccgcctgagc gcgcaattga actggcgggt cgaggcctca gcggccgccg ccgagccgtc
    32341 acatgtcacg gtgcaggtca aggaagcgca gtttgccaac gccgacctgc agggcgagct
    32401 gcgcgggagc tggtccaccg ggttgtttgc cgcgaatggc aagcacagcc ctcgttatcc
    32461 cgggctgctc gacctcgatg ccaagctgac gcaggtgacg gctgcgcgtg tcttcaggta
    32521 tctgccactc ggcgtctcgc aagaggcgcg gcgctatgtc gagcgtgcgg tccacgcagg
    32581 ccgcgtggcc agcgcgacct ttcgcgtcag gggcgatctc aacgactttc cgttcgcgca
    32641 gggcaacgcc aaggacagcg aattccgcat tgcagcccag atcgacaagg catcgttcgc
    32701 ctacgttccg agctggccgg ccaccgtcac ggagccggcc tatgtttcga cctggccgga
    32761 gctcgaggag ttgactggcg agctggtgtt cgaccgcgag tcgatgttga tccgcaatgc
    32821 ccaggcgcgt attcagggtg tgaagctcgg cggcgtgcaa ggtggcatca agacactgac
    32881 cgacaagtcg gagctggtgc tcgagggcgc tggccgcggc gcggcggccg acatggtgcg
    32941 ctttgtcaac gtatctccgg tcggcggctg gaccggcaac gcgctggccc acagcacagc
    33001 gtccggcagc gcagacctga agctctccct ggcggtgcct ctggcggatc cggcggctgc
    33061 cacggtgcgt ggcagtgtga cgctggcggc caccgacttc cgattgcggc ccgacacgcc
    33121 acaggttggc gcggtgcgcg gccgcgtcga cttctcccag aaggggtttt ccatcgtcgg
    33181 ggcttccggg cgcttgctcg gcggcgacct gagcgtcgac ggcggcctgc agcctgatgg
    33241 ctcgatgcgc tttgtgggcc agggaatcgc aactgccgat ggcctcaggc gtggcggcga
    33301 tctcggcggt gctgcgctgg cccggctcgc gaactcgctg agcgggcagg ccagctaccg
    33361 cgtcgcgctg aatctggtgc gcggacagtc ggacctgctt gtcacgagca acctggtggg
    33421 cctgggaagc gaactgccgc cgccgctgcg caaggctgcc gattcgcctc tggcgctgcg
    33481 cttccaaagc gcgccggtga ccgatgccgc ggccggcagc ggttcagcct ctgcgacggc
    33541 gctgcaggac agcgtccgcc tcgacctcgg caatctgctg cagctgcact acgttcgcga
    33601 cgtgtccggc gcgttgcccc gggtgctgcg tggaggcatc ggcgtgatgg agccggcgcc
    33661 gaccccggcc aacggagtca gtgccctgct gaacctgggc aacctgaacg tcgatgcctg
    33721 ggagcaggtg gctgccagcc tcaccggccc ggcggccggt ggttccggca acgcgggcgg
    33781 cagcgccatg ctgggtcctt cgggcgggga tgcagcctcg gtcttcagcg cctacctacc
    33841 gaccacgatc ggtctgcgcg cacaggagct gcattccggg tcgcggcatc tcagccgctt
    33901 ggtggcgggc ctctcgcagg agggcacggc gtggcgggtc aacctcgatt ccgatcaggc
    33961 cagcggctac atcgagtacc gggcaccgcg cgcatcgtct ggcagcgctg ccgcggcccc
    34021 gatgagcgcc gccgcggccg ggcgggtcta cgcgcggctg agtcggttgt cgttgcccaa
    34081 ggacgacgtg gccgacgtcg agaacctgct cgaccagcct gcggcgcaga gcgtgcctgc
    34141 cctggacatc gttgtcgacg acctggagct gcgtggcaag cgtctggggc gcatggaact
    34201 ggaggccacc aaccgccagg gcgcgcgtga atggcgactg acccggcttg cgctgaccac
    34261 accggaagcg aagctgactg ccagcggcaa ctgggcggcg ctgggagccg gcgcgggggc
    34321 cgcgtccggc gccgtgtctc ccgcggcgcg acgggtcgtg ctgagtttca agctcgaact
    34381 gagcgacagc ggggcgctgg tcgaccgact gggtttcggc aaggtggtgc gaggcggcaa
    34441 gggcgggctg tccggccagg tcgcgtggtt cggctcgccg ctgtcgctgg actatcccag
    34501 cctgaccggc cagatcaaca tctcgatcgc cgcgggccag ttcctgaaag ccgaaccggg
    34561 cgtgggtcgc ttgctcagcg tgctcagcct gcaggcgctg cctcggcggc tggtcctcga
    34621 tttccgcgac gtgttccagc agggcttcgc cttcgacgac atcagcggcg acgtgcaggt
    34681 cacgcaaggc gtggcgcaga ccaacaacct gcgcatgcgt ggcgtgcagg ctgccgtact
    34741 gatggaaggc aaggccgaca tcgagcagga aacgcaggat ctgcgagtca tcgtggtgcc
    34801 ggagatcgac gccggcacgg cctcgttggc ctacgcggcg atcaaccctg cgctgggtct
    34861 cggcgccttc ctggcgcagg cgctgttcag caagccattg atcgcagcca acacgcgcga
    34921 gttccacatc acgggcacct ggggtgaccc caaggtcgac aaggtcgacc gcaagttcgg
    34981 ggcgccggtg cccgacatcc ccgcacccag ctcgtcgatg ccctccggcg cgccggctgc
    35041 agccgccaac gaatcgccac cgaagccatg aagatcgctg ccctgcagat ggtgtcgaca
    35101 ccttgtgtcg agcacaacct ggccaccgcc ggtcgcctga tcgcgcaggc cgccgatgcc
    35161 ggagcacgct gggtggcgct ccccgaatac ttctgtctga tgggacacag tgaccgcgac
    35221 aagctggcca tcgccgaggt ggccggggcg ggaccgatcc aggacatgct ctccggcgca
    35281 gcacgccgcc acggggtgtg ggtgattggt ggcaccctgc cgatccgttg cgacgagccc
    35341 gagcatgtgt tcaatgcctg ttgcgtctac gcgcctgacg gcaccatggc tgcgcgctac
    35401 gacaagatcc atctcttctg cttcgacaac gggcgcgagc gctacgacga aggtcgcgtg
    35461 ctgcgcgcag gcagtgaacc ggtggccgtc agcggatcgg ccgatgggga catcgctgca
    35521 ctgcgggtgg ggctcagcat ctgttacgac ctgcgctttc ccgagctgta ccgggcgctg
    35581 atgacgatgc ccggccagcc accgtgcgac ctgatcgcgg tgcccgcagc attcacccac
    35641 acgaccggcc aggcccactg ggagttgctg ctgcgcgcgc gcgcggtcga gaaccagtgc
    35701 tacgtgattg cggccgcgca gggcggcctg cacgagaacg gccggcgcac ctggggccac
    35761 agcatgatcg tcgacccctg gggcgagatc gtcgcggtgc acgccgaagg cgagggagtc
    35821 gtgattgccg accttgatct cgatcggctg gcagcggtcc gcttgcaact gcctgcgctg
    35881 aagcaccgcc gcatcacctg aacaccgccg gcggcgtcag accttgctga acagcaggtc
    35941 ctggacgacg accgcgcgca cgggagcttc gtgcctggct gcgaggaact cgttgagacg
    36001 ccgttccatg tccttcgaca gtttgttcat gccgcccgca cgctgcagcg tcgggcggtc
    36061 atgtgtcccg aggcagatct gcagcatcat cgcgacctgc gtccggttct cttcgaggga
    36121 ggcgcggctg gatcggtcgc tcgcgtcgat cgcaacccgg gccttgaccg attcgccgtc
    36181 gctggtggtc gcctgtaccg attcggatcg cacccagttc ggtgctgcct ggccggaggg
    36241 cgtgggttcc gaaacgcgga cccacgacac gccgagcatc actgcagccg ggacaccgat
    36301 caccaggatg acgccggcca gaacgtaggc aggcaacatc cggtcgctgg cctgcggctt
    36361 tcgaggcccc tgggctggcg gcgcggtgcg cgtgcgttcg ggtcgggcca tggtcgattt
    36421 gcgattggcg tgcggacgcg gccaatctaa ccgccggaag gggccgcgaa cggttcgggt
    36481 gccgcggtct cgtgcgaagc atcacagcag gtgccggcga gagtgccggc ggtcgagcct
    36541 gcgtgcgcct cgaggctctc caggtccggc gctggtcact cgcaggcagc gatcacctgg
    36601 gccaccgctg cggtcagctt cttgccgtag ggcacgtgga ggaactcgtt cggcccgtgc
    36661 gcattgcttt tcgggcccag cacaccgcaa accatcatct gcgcgtcggg gaagccctgt
    36721 tgcagcaggc tcatcagtgg gatggtgccg ccctgtccga tgtagccgca gggtgcaccg
    36781 aattgagttc gcgacgcagc gttcaacgcc cgttcgagcc agggagccag cgccggggcg
    36841 ttccagcctg tcgcgcccga aggttgccct ccgccgccgc cgcccgagcg gccatcggcg
    36901 ctgaacgtca ccttggcgtt gtacggcgcg ttgtcctcga gcagcgcctt cagcctcgca
    36961 gcggcttcgt tgccatcgat gagaggtggc aggcgcaggc tgagcttgaa tgcggtgtag
    37021 ggcctgagca cgttgccggc actgcgcatt tccgggaagc cgtcgactcc ggtcacgctc
    37081 agcgtcggct tccatgtccg ccggatcagg gcttcgaacg ggtcgctggt ggtcggcagc
    37141 gtcgggccgt cgtcggcgcc acaggcccac gggaaacgtt tccacagctc gtcgccgagt
    37201 gccgcggcgg tggcccgcgc ctgttcgacg cggtctgccg gaatctcgca atggaagcct
    37261 tcgggcagca attcgccggt ccgggaatcc tcgagccggt cgagcaggtg acgcaggatc
    37321 cggaagctcg acggcaccag cccgctcgag tcgcccgagt gcaccccttc cgtcagcacc
    37381 tcgaccttca gcacgccgct caccatgccg cgcagattgg tcgtcagcca cagctggtcg
    37441 tagttgcctg cgccgctgtc gaggcacacc accaggtcga cgcgtcccag gcggtccttc
    37501 agtgcgtcga tgtaggcggg caggtcgtac gagccgcttt cctcgcagct ttcgatcagg
    37561 cccaggcacc gtggtcgcgg gatgccctgt gcgtcgagcg cccggatcgc ggtcagcgcg
    37621 gcgtagaccg cgtagccgtc gtcggcgcct ccgcggccgt acagcaggcc gttctcgtac
    37681 ttcggcgtcc agggcccgag gtcgtggcgc cagccggtga actcgggttg cttgtcgagg
    37741 tggccgtaca tcagcaccgt cccggcatcg ctcgcgcttc cgccggggcg cgtcgccggc
    37801 gcgtcgaaga agatcaccgg cgtgcggccc ggcaaccgca ccacctcgag cttcagcccg
    37861 gccacggcct gccgttcgac ccactgcgcc gcgtcgcgga tcacgcgctc gatgtgcccg
    37921 tgcgactgcc attcggcgtc gaacatcggg ctctttgcgg gaatggcgat gtaatcgacg
    37981 agcgcaggga tgatggcttc atcccaggcg cgatcgcaga aagcggcgat cgcggtcgcc
    38041 tggtccgggg cgccgccttc gggccggtgc agggcgggtt cgggagcgtt catctgggga
    38101 ctccggtgcg gctcaggtga gcgtcgggct cagtgtagga ccgcgccgtc cgcctcgatc
    38161 ggctgatcgt gcatggcctt gaagcgcagc gtgaacctta cgcccgggcc gaagggtgcg
    38221 cccgcctgat cgttcagtcc cgagcggtgc cgcaggttgg cgtcttcgag cagcagctcg
    38281 gcgccgtgct gggcggcgat ctggcgaacg atcgccagtc cgaggccgga gccatcgacc
    38341 tgcgtgccga gcacccggta gaacggctgg aatacctgat cgcgttcggc gggcggaatg
    38401 cccggtccgg agtcctcgac ctgcagcacg accacctggc cgaacgggtc gtcgatgaca
    38461 cgaaccgtga ctgtgccgcc cgagggcgtg tagcgcagcg cgttgtcgac caggttgcgg
    38521 atcagctcgc ccagcagcac gcggtgtccc cgcaccttca ggccgccggt cgccggcgct
    38581 tcgaggctcc cctcgacatc gcgtcccggg ccttcgtagc ccagatcgat gcgcttctcg
    38641 agcgcgcggg gaacgaaatc gcgcaccgtc tcgacggcca gttccgcgag atcgaccggg
    38701 ccgaggttca gcgcctgttc cttgtcatcg gcgcgtgcca tcgagagcag ctggttgacc
    38761 gtgtgcgctg cgcgctggct cgacagcgcg atctgctgca gggagcgctt gatgtctgcc
    38821 ggatcgttct tgccggcatc gatgtcgcgc tgcgccagtt cggcctgcat gcgcaggccg
    38881 gccagcggtg tcttcagctg atgcgcggca tcggtcagga aatgcttctg cgaactgatc
    38941 gaacggtcga gtcgagccag caggtcgttg atcgcacgca ccagcggcga cacctcctcg
    39001 ggcgcttcgc gctcgtcgat cgggctcagg tcgctgctat cgcgcttgcg gatgcgctgc
    39061 tgcaattcgt tcagtggtgc gatgccgcgg gcaagcgcca gccacaccag caggaccgcg
    39121 agcggcagga tcacgaactg cggcaggatc acgcccttga tgatctcggt ggccagccga
    39181 gagcgcttgc ccagggtctc ggccacctgc accagaggcg ccgcatcggc cgggcgcccg
    39241 ggcacgtcga cccacatgta ggccacccgc acgttctcgt cgtggatcgt gtcatcgcgg
    39301 tagcgcagtt cgccgggagg cacgcggtct tcctctgccg gcacggccag cgtgctgtcg
    39361 ccgctcaggt attcgccgcg cagtcccagc acctggtagt acacatcgtc cgcttcgtcg
    39421 gcgcgcagga tctccgatgc gccggcgggc agctcgaagg ttgccgctgc gtatccctgg
    39481 ggcgagctga cgacgacctg cttcgacagc acccgaacca tgtcggcgag ttcgcggtcg
    39541 taaggctggt tggctatgcc ttgtgcgacc agccaggtca gcaccacgct catcggccac
    39601 agcaccagca gcggcgcgag catccagtcg aggatctcgc cgaagaggga acgttgttcg
    39661 tgtggtcggg ccatgaagag tcaaagctgc tcaggccgcg attttctcca ggcagtagcc
    39721 cagtccgcgc acggtcgcga tgcgcaccgg gccttgctct atcttcttgc gcagacgatg
    39781 gatgtagacc tcgatcgcgt tgttgctgac ttcctcgccc cattcgcaca gccgctcgac
    39841 cagctggtcc ttgctcacca gccgtcccgc gcgctgcagc agcacttcga gcaggctgat
    39901 ctcgcgagcc gacagctcga tcatctggtc gttgatgtaa gccacgcggc cggtggcgtc
    39961 gaaggtcagc ggcccgtgct tgatgacgct cgatgcggtt ccgagcccgc gacgcgtcaa
    40021 ggcacgcacg cgagcctcga gctcctgcag cgagaacggc ttggccatgt agtcgtcggc
    40081 accgaggtcc agccccttga cgcgctggtc gacgctgtcg gcggcggtca ggatcagcac
    40141 cggcacgctc gagccgcgcg ctcgcagctt gcgcagtact tccaggccgt gcaccttggg
    40201 cagtcccagg tcgaggatca ccatgtcgaa ctcgtgcgaa gccagcgccg catccgcgtc
    40261 gctgccgctg ttcacctgat ccaccgcgta gcccgcgttg cgcagcgagc gcagcaggcc
    40321 gtcggccagc acctggtcat cttcggcgat cagaacccgc atccgtttgt ctcccctggc
    40381 agatccatca tcgcgccgcg ccgcgcgtca gggaagttta gggttccggc ggggtctgtc
    40441 ggcggacatg ctgatggctg tatacccgta cgaatcgccc gaatgggggc accacggggg
    40501 tcgatcgacg ggatgtcgaa cgccaaataa aatactgtac aaacatccag cttttgcaat
    40561 aatcccatct acaccccgga gaacacccat ggacgcacct gtcaaagccc tgaacaccga
    40621 aaaagccaaa gccctgcagg cggcgctggc gcagattgaa aagcagttcg gcaagggcac
    40681 gatcatgcgt ctgggcgagg gtgaggtgat cgacgacatc gaggtggtgt ccaccgggtc
    40741 gctgggcctc gacatcgcgc tgggcgtcgg cggcttgccg cgcgggcgcg tcgtcgagat
    40801 ctacggcccc gaatccagcg gcaagaccac gctgaccctg caggtcatcg ccgagatgca
    40861 gaagctcggc ggtacctgcg ccttcatcga tgcagagcac gcgctcgaca ccgcctacgc
    40921 ccagaagctc ggcgtcaacc tccaggaact gctgatcagc cagcccgaca ccggcgagca
    40981 ggcgctggag atcgtcgatg cgctggtgcg ttccgcttcg gtcgatctgg tcgtcatcga
    41041 ctcggtcgcc gcgctcacgc ccaaggccga gatcgaaggc gagatgggcg actcgctacc
    41101 cggcctgcag gcacggctga tgagccaggc gcttcgcaaa ttgaccggca cgatcaagaa
    41161 gaccaacacc atggtcatct tcatcaatca gatccgcatg aagatcggcg tgatgttcgg
    41221 tagccccgaa accaccaccg gtggcaacgc gctgaagttc tacgcctcgg tgcggctgga
    41281 catccgccgc atcggcagca tcaagaaggg cgaagaggtc atcggcaacg agaccaaggt
    41341 caaggtcgtc aagaacaagg tatcgccgcc gttcaagacc gccgagttcg acatcctcta
    41401 cggcgaaggc atcagccgcg aaggcgagat catcgacatg ggcgtcgaag ccaaggtgct
    41461 cgagaagagc ggagcgtggt atgcctacaa gggcgagaag atcggccagg gcaaggacaa
    41521 cgcgcgcgaa tttctgcggg agaacccggc gctggccttc gagatcgaga acctggtacg
    41581 cgaggcggtc ggcatcccgt ccctgccgcc gctggacggg agcgacgccc ccaaggccgc
    41641 caaagagcgt ccggtcaagg agtaggcacg tggtggcggt cgcacgtccg ctgagatgaa
    41701 gccgccgctg tctctgaagg ggcgcgcgct gcaatggctg gcccagcgcg aacacagccg
    41761 cctggagttg cgccgcaagc tgctgcagcg gttgcgcgac gatcaccgcc acgactgccg
    41821 accgcccgat ggcgaggaag ataacgggct gacaaccgac gctgcagcgt ccagcaacca
    41881 tcccgaggtc aaccccgaga ttgaggtgga tgcgctgctc gactggttgc aaagccaccg
    41941 ctacctgagc gacgagcgct tcgtcgaaag ccgggtccac gcccgctcgg cgcgctacgg
    42001 caatctgcgc atacgccgcg agctgtctca gcacggtgtc gcgctgacat cggccctcga
    42061 tggtgacctg cgcggcagcg agcaggcgcg ggcggtcgag gtgcaactgc gcaagtttgg
    42121 cggaccgcca gcttcactcg tcgaacgggc gcggcaggcg cggtttcttg cttcgcgcgg
    42181 cttttcgtca gatgtggtgc agaaggcctt gcgcgcgccg cgcggtcgag acgcggcagt
    42241 cacgaccgct ggcgaggacg cttcagccgt gcggctgcat tcagtgcccg acgacgatgc
    42301 ggattgaagc gaattcgcac ttcccggttg cccgccgtgc cttgtctccc tgactacccg
    42361 gttggtgcac tgcagcatgt tacattcccc gcgctaatcg ccaggggtcg cggctgtggt
    42421 cgatgcatca tctgtggtcc gtccgagctg atgaactgca ccgacagcga cggttgcgac
    42481 tgaagccttc gatttcagca tcgctgtgat gttcggtcgc tcgaccggca tcgcagctga
    42541 aacggccttt tctcctctca ggcacttcga cttcatcttc tgcaagaaag cctccgcatg
    42601 aaaatccatg aataccaagg caaagagctg ttgagccgct atggcatccc ggtgcccaag
    42661 ggctacgcag cgttcagcgt ccatgaggct ttggaggctg cgcagaagct tggcggtccg
    42721 gtctgggtcg tcaaggcaca gattcacgcc ggcggtcgcg gcaagggtgg tggcgtcaag
    42781 ctggcccgct ctctcgacga tgtcagcaag ctggccggcg agatcctcgg catgcagttg
    42841 aagacacacc agaccggccc cgaaggccag aaggtgcgcc gactgctggt cgaggaaggc
    42901 gccgacatca agcacgagta ctacgtcgca gccgtcaccg accgcgccac gcagaaagtc
    42961 gtgctgatgg cgtcgagcga gggcggcatg gacatcgaac aggtcgcgca cgacacgccg
    43021 gagaagatca tcaaggtgtt cgtcgatccg ctgatcggcc tgaccgatgc gcaggggctc
    43081 cagctcgcag ccggcatcgg catccccgcg gccagccagt cccaggcggt cgatgtcttc
    43141 aggaagctgt atacctgcta catggagacc gactccagcc ttgccgagat caacccgctg
    43201 atcctcgaag gctcgggcaa catcaaggcg ctcgacgcca agttcaactt cgacagcaac
    43261 gccctgttcc ggcatcccga gatcgtggcc taccgtgatc tggacgagga agacccggcc
    43321 gagatcgaag ccagcaagtt cgacctggcc tacatccagc tggacggaaa catcggttgc
    43381 ctggtcaacg gtgcaggcct cgcgatggcc accatggaca ccatcaagct gttcggcgcc
    43441 gagccggcca acttcctcga tgtgggcggc ggcgccactg ccgagaaggt gaccgaagcc
    43501 ttcaagatca tgctgggcaa tccgaaggtc aaggccatcc tggtcaacat cttcggcggc
    43561 atcatgcgtt gcgacacgat cgccgagggc gtgatcgccg cgtgcaaggc ggtcaacctg
    43621 aacgtgccgc tggtcgtgcg catgaagggc accaacgagg acatcggcaa gaagatgctg
    43681 aaggattcgg gtctgccgat catcagcgcc gattcgatgg ccgatgccgc gcaggccgtg
    43741 atggccgcgc tgaagtaagc gccccgagac caaggacata cacatgagca tcctgatcaa
    43801 caaagacacc aaggtcatta cacaaggcat caccggcaag acgggccagt tccacacccg
    43861 catgtgccgc gagtacgcga atggcaagaa ctgcttcgtc gcgggcgtca acccgaagaa
    43921 agccggcgag gacttcgaag gcatcccgat ctacgccagc gtcaaggaag cggcacagag
    43981 caccggcgcg accgtcagcg tcatctacgt gccgcccgcg ggcgcggcgg ctgcgatctg
    44041 ggaggcggtc gaggccgatc tcgatctggc catctgcatc accgaaggca tccccgtgcg
    44101 cgacatgctc gaagtgcgca acaagatgat ccagaaggaa gccaagggcg gcaagaagac
    44161 gctgctgctc ggccccaact gtcccggcct gatcacgccg gaagagatca agatcggcat
    44221 catgccgggc cacatccacc gcaagggccg catcggcgtg gtcagccgct cgggtacgct
    44281 tacctatgaa gcggtcgcgc agctcaccga gatcggtctc ggacagtcca gcgccgtcgg
    44341 catcggcggc gatccgatca acgggctgaa gcacatcgac atcatgcggt tgttcaacga
    44401 cgatcccgat accgatgcgg tcatcatgat cggcgagatc ggcggccccg atgaagccga
    44461 ggccgccgtc tggtgcaagg ccaacatgaa gaagccgatc gtcggattca tcgccggtgt
    44521 caccgcaccg gccggcaagc gcatgggcca cgctggcgcc ttgatctcgg gcggtgccga
    44581 cacggccgat gcgaagctgg ctgtcatgga ggcctgcggc ttcaccgtga cgcgcaatcc
    44641 gtccgaaatg gccaagctgc tccagaagct gctctgatgc cagagacgcg ccgggcttcg
    44701 gcgattcacg cgccgccgcc tggtgcgcct cttgcccggg cactgctcgg gcatcccgtg
    44761 ctcgacgcag aattcctgcg tgacccctac ccgacctacc gggttctgcg cgaaggcgca
    44821 ccgctggtct ggagcgacga attcttcggt ggcgcctggc tgatgtcgcg ccacagcgac
    44881 gtcgagatgt tgttccgtga tgcgcgtttt tccgcgcaac gcgccggggg ctgggtcatg
    44941 cgcaccgagg aatcgcgcac cgagctgcgc gggttccaaa ggctgtttgc ccgggcactg
    45001 gtctttctcg atgcgcccga ccacacgcgg gtgcgtcagg tgctcgcgac cggcttccgt
    45061 ccgtcggatc tgcagcggct cgcgccgcgc atcgagcagg cggttgccga acgtctcgat
    45121 gccgtcacgc ccgcccgcct ttttgatttc gtggcgtctg tcgcgcgacc gttacccgcc
    45181 caggtgatcg gaatgctgat gggcatcgac gacgagccgt ccgggttcgt tgcctggtct
    45241 gacgcgctgg ctgatttcgt cggcgctccg gacccgagcc aggcgcaggc gctgaccgct
    45301 caggccgcac tgctggagat ggctcgctat ttcgaatcgc ttctgactcg tcgccgagcc
    45361 gcgctcgatg gaggcgatga accgcgcgat gacttgatcg agcgcctgct ccaggccgag
    45421 cgctcgggtc agatccacgg aagcgccgaa ctgatggcgc aatgcgtgat gctgctgttc
    45481 gccggacacg aaaccacgcg ccacctgctg gccaacggcc tgcgcatgct gctggcgcat
    45541 cccgacgcct ggcaccatct gcgccgtgac cgcgccctga tgcccggggc cgtgcgcgaa
    45601 ttgcttcgct tcgacagccc ggtccagtac accggccgcc gggtggtcac cgatctggtg
    45661 ctgcacggtc aaaccctgcg ccggggcgat ctggtggtgg cactgatcgg tgccgccaat
    45721 cgggaccccg ctcgccacga gcgtcccgac gagctggaca tctcgcgcag tgccggcgcg
    45781 ccgctcgcct tcgggcaggg gccgcacgcc tgcatcggag ccgcgctgac cctgctggag
    45841 gcgcagacgg tcttctcgca ggtgatcgat cgctggcccg acctcgctct ggcagagccg
    45901 aacgccgcag cgtggaccgg caaaccgctg taccgcggcc tgaccgaact gcggctgaat
    45961 tccgccgggc cgtcgtcacg gtcagccgat gtcggcgcct acactgccgc ctgaaatcac
    46021 aagagcccac ccagagaccg catgcaagcc ctgatgaccc ccgagttctg gatcgctgtc
    46081 ggccagatca tcatgatcga catccttctc ggcggcgaca acgcggtggt gatcgcattg
    46141 gcctgccgaa agctgccacc ggcccagcgc accaagggca tcctgtgggg caccgccggc
    46201 gccatcgtgc ttcgagtgat cctgatcttc ttcgcgctgc agttgctgtc gatcccgttc
    46261 ctgaagctcg tcggcgcgat cctgctcgtg tggatcggcg tgaagctgct gcttcccgaa
    46321 gatgaggacg gacacgccca ggtcgagggc agcgatcggc tctggggtgc cgtcaagacc
    46381 gtcatcgtgg ccgacctggt gatgagcgtc gacaacgtca tcgccattgc cggtgcggcc
    46441 gagacggccg gtggcgatca cacgatgccg ctggtcatct tcgggttgct ggtcagcatt
    46501 cccatcatcg tctggggcag ccagctggtc atcaagctga tggaccgata tcccgtcatc
    46561 atcacgttcg gggcgatgct gctgggatgg atcgctggca gcatggcggt cagcgatcct
    46621 gcggtggtca accccgaggt gctgccgcag ctgcccaagc tgatcgtgag cgatggtgtc
    46681 aagtacggtg ccggcgcggc cggtgcgctg ctggtgttgg ccatcggcaa ggcactgcag
    46741 gcgcggcgcg gagtcagggg ctgatgtccg tcctcgccag tgcaggcgca gcgtttctcg
    46801 ctggaggatt gctggccctg tcgctcgcgg cctacctgtt tttcgcgttg ccacgtctct
    46861 cgtcgacggt ggcatggatg gtcaccggaa cgctgagcct ggtcctgctc gcgggtgggg
    46921 tctacggctt gtgggcggcg tcgggttcct cggcctgggt cgcggtgctg ctggcttctg
    46981 caggtgccct gctggccggt cacgcgggct ggtcgctcgg ccacgctgcg gggcgcatcg
    47041 cggcctcaca cctcccggca ccggtcgctc aggctgcggc acccgcgccg gcgaccttgc
    47101 ggccgacggc ggactcgcct ccggccgcgc cgcgcgttcc cgagcacgcc aagctcggtc
    47161 gctaccgcat cgagcgccag ctggggcgcg gcgccatggg tgcggtctac ctggggcgcg
    47221 acccgcagat cggccgtcag gtcgcgatca agaccatggc gctcgcctcg gagttcgaag
    47281 gtgcggaact cgtcgaggct cgtcagcgct tctttcgtga agccgaaacc gccggcaggc
    47341 tgcagcatcc cgacatcgtg accatcttcg atgcgggaga ggaccaggaa ctggcctaca
    47401 tcgcgatgga attcctgcgc ggtcatgacc tgcagcgcca cacgcagtcg gcccagttgc
    47461 tgccgatgcc gctggtcgtg cacatcggcg agcgcgtggc caacgcgctg gcgcacgcgc
    47521 acagccaggg ggtggtgcac cgggacgtga aaccggccaa cgtcatggtg gacttgccga
    47581 acggcgtcgt gaaggtcacc gatttcggca tcgcccgcat caccgattcc agccgcaccc
    47641 ggaccggcat ggtgctcggc acgccttctt tcatgtcgcc cgagcagatg tccggtcgcc
    47701 gcgtcgacgg gcgtgccgac ctctattcgc tgggtgtcat gctgttccag ttgctgaccg
    47761 ggcagctgcc gcatcgtgcc gagtcgatgg ccaagctgat gtacgccatc gccaacgagc
    47821 ccgcgcccga tctgcgcacg ctgcgacccg agattcccga ggcgctcgcg caactggtcg
    47881 cccgtgcgct ttccaagcaa cccgaagccc gccatgccga cggccggcag atggctgcgg
    47941 cgctgcacga cgtggcggcc ctgtggcccg acagcgcgct gatcgtggct gggctgccga
    48001 cttccgcggt cgcggcgcag ccggaacacc ccgattcgga tgctttcgct gcgacggtga
    48061 agatctcccg cactgatccg gggcacaatt ccagcttgtg attgcccttg cgcgcgtcgc
    48121 ttgcggacct cggcgttcgc actgacgcac cgcagttcct tgatcgtgcc ggtccgaaac
    48181 ttatgccgtt cgagtttttc agcgccaccg acaccggacg cgcgcgcaac aacaacgaag
    48241 actccgtcgc ggtcgatgcc gtttcagcac tggtcgtgct ggccgacggc atgggcggct
    48301 acaacgccgg cgaagtggcc agcaacatgg ccacctcgtt catcctcacc gaacttggcc
    48361 gctggctgac cgaggcggcg tccaatgcga ccgatgccga tgttcggcgt gcgatggaca
    48421 tctgcgtcga caacgccaac cgcgcgatct tcaatgccgc caactccaat ccgcagtacg
    48481 ccggcatggg cacgacgctg gtggtcggcg tgttccgcga aggtcggctg ctgctcgggc
    48541 atgtcggtga ttcgcgcggc taccgcctgc gcgatggcgt gttcacgcgc atcacgcacg
    48601 accattcgct gctgcaggag cagatcgacg ccggcctgat caccgaagag caggccgcgt
    48661 tctcgtccaa caagaacctg gtcacgcgcg cggtgggtgt cgaggacgcg gtcatgctgg
    48721 aggcgcacct ccacgacctg cttcccggcg acctcatact gctgtgctcg gatggcctgt
    48781 ccgacatgct tgacgacgag cggatcggcg cgctgctgca atcgtgcgaa agcatcgaat
    48841 ccggtgcgcg cacgctgatt tccgcggcca acgaagccgg cggaaaagat aatattgcgg
    48901 tcattttggt gcgcgttcag ggcggggccg gggccgggcg atcctggtgg ccgttcaagc
    48961 gctgagtgca ccgcgcgtat tcgtttcgac cgatcgagca gacggtttcc accaagtaga
    49021 gacaacaaca gacgaggtca agcatgggca aactggtggt atcgctcgat ggcgtggtga
    49081 tcaaggaagt acagatcacc aaggacaaga ccaccctcgg ccgccgtccc tacaacgaca
    49141 tcgtcatcga caacctggcc gtcagtggtg agcacgcggt gttgcagatg ctgggcgcgg
    49201 acgtcttcat cgaagacctc aacagcacca acggcacgta catcaacggc aaggccatca
    49261 agaagcagct gctggtgcac aacgacaccg tcgagatcgg caagtacaag atcaagttcc
    49321 tggtggccga tggcaccgac tacgaagcga cgcagatcat gcggccggca tcgcacgctg
    49381 ccgccgcacc ggtgcccccc ggcctgcagc cggcttccac gttcggctcg ctcggtgctt
    49441 cggcctcgat caaggtgctc aacggcgccg ccgcagggcg tgaggtgtcg ctgaccaagg
    49501 tggtcaccac catcggaaag ccgggtgtgc aggtggcctc gatcaccaag cggcccactg
    49561 gatacgtgtt cgcgcacgtc gagggcgcgg cgcggcccac ggtcaatggc aatccgctgg
    49621 tggccgagac cacgcagttg aaggatggcg acgtgatcga actggccgga acacagatgc
    49681 agttcatcaa ccgctgaact ccccctggca gccagccgct atcgcgcgtg ctggccgtga
    49741 cgcttttcac ggcgtcgcct gcgggttgat tggccggggc ctgaggcccc ggcaaacgag
    49801 acttccgcac cttgtgggcc cggccgaccg gggtcagcat ccacggcacg cgttttcgcg
    49861 tttctggctg cacccttgtc cgtcactttc cacacaccat gaccctgcgc gtgctcggct
    49921 gttccggctc cattgcggct gggtgcaaga ccacctcctt cctgctcgat gacgatgtgt
    49981 tgatcgatgc cgggaccggg gtcggcgacc tgccgctcga tgcactggcg cgtatcgatc
    50041 atgtgctgat cagtcactcg caccttgatc acgtgctgtc gattgcactg ctcgccgaca
    50101 gcgtgatgcg cgtgcgcgcc gctcagggtc gtggaccgat ccaggttcat gccttgcctg
    50161 aaaccctgca ggcgctgcat cagcacattt tcaatggcgt catctggccc gatttcactc
    50221 ggctgcccac gccggaccgc ccggcgttgc agctggtgcc gttctcggtc ggcgaggtgc
    50281 tgaccgtggg tgcgcgcctg cgccgtgtcg aggtcctcag cgctgcccac accgttccgg
    50341 ccgtcggctt tgccgtcgag ggcacgcaga gcgacggcag catttgctgg tgggtctaca
    50401 ccggcgacac cggccccaac ccggcgctct ggaatcgcct ccgctcgatg aacgtgtcgc
    50461 acctggtgat cgagacggcc ttcagcaacg aagagcagca actcgcccag ctcagcagcc
    50521 acctgtgccc gaccacgctc gcccgcgagc tggtgcagct ggctggtcgc gtcgatgtgc
    50581 gcatctccca catcaagccg ggcgagatgt tctcggtgat gtccgagatc ggccggcttg
    50641 cgatgcgcca tcgcgtgcag gcgctggcca tcggcgctgt catgtcgctg ggctgatcgc
    50701 gagtcagtac gcaggcggtt tatcgcctcg cgtgccctgc tctgatctga caatttgcgt
    50761 cacctcgaag ccccggtcgc ctgctttctg ccccgaaggg tgaccgtttg cgtcgccgag
    50821 ggccgccaaa ccggcagtat tcacggcaaa accggtggca tgcagcctgc aactactcct
    50881 tcgtcttcac cccccatccc tcaaggagtt caccatgcaa cgtcgcatcc agcaaggttt
    50941 caccctcatc gaattgatga tcgtcgtcgc gatcatcggc atcctggccg cggttgcgct
    51001 gccggcttat caggactaca cggttcgcgc taaggtttcc gaagtcgtgt tggcaggctc
    51061 ggcatgccgc accacgatca ccgaggtcta tcaatcgtca gactctggcg cgtcagtcgg
    51121 cgccaattcg tggggctgcg aaaccgacgc ttccgctgct gcaggccaat caaaatatgt
    51181 tgcatctgtc gtgacagatg acgacggcat ggtcacgatc acggcgcaag gcattcccca
    51241 ggttgacggt cagaccgttg agttggagcc acaggattcc accggtacga agatgactgt
    51301 cgcgaccaac atcgggcaag cagtgtttca atggaaatgc cgtccaggca cgatgaccgc
    51361 caagtacctt ccaggttctt gccgctgatc gagtcttaac tcgccgatca gaaaggggca
    51421 gcgaaagctg ccccttttca ttcccgtaag cgattcgagg gcacgatgtt cgagctactg
    51481 ggttctgccg cgatggtgat cgcctggctg gttccagacc actatcttcc ctggctctct
    51541 gcctacaacg acagtgtcat ggcgctggcg ttgatccttc tactgctggg tcttgtgaag
    51601 cgtgaggcat cacaggccag cgtgccagcc atcttctgga tcactgtcgc ggtcgccggc
    51661 attccttggc ttcactgggt cacaggtcgg ctcgccttca gcggagacgc tgtggttggt
    51721 agtctttatg tattggcgct ggccattgcg atcctggtcg ggcacggctg ggctcgccgc
    51781 gaccccactg gttcggcggc cttgctcgcc ggaacgattc tgcttggtgg ctcgctttcg
    51841 gccgttattg cgctctttca ggggctgagg gtcggcgatc tcggactctg gagtcttggg
    51901 ctgtgcaccg gtggccgagc ttgtgccaac ctcgcccagc ccaataacct cggtaccctt
    51961 ctgggcctgg gcgtgctcgg attgctgctg cttcgagagc agcggcgcct cgggcgaggg
    52021 gtgagcgcgg ctgtgcttgc actgttgctt ctgggggccg cgaccaccca gtcgcgcacg
    52081 gccatgctgt ttggtccggc catcctgatc gggctgttcg ttgcgcgcag gcgtggtgtg
    52141 cagtttcaaa ttcagcttcg aacggttttt gccgtgaccg tgattcactg gctgctttcc
    52201 tacgggtggc ccgtgctgca gaccgccctg ttgctcgata ccacggcatc gctcagtgcg
    52261 cgcagccttg acgcgggtcg cttgcaggca tgggtgatgt tcatcgatgc gctttcccaa
    52321 tcgccctgga cgggtgtggg ttggatgcag accgccagtg ctcagcttgc ggtcgcagag
    52381 aaccatccac ctctgcgcct ggtgtggcag cacgcgcaca acctgttcct ggaactgatg
    52441 ctttggtgcg gctatccgct gggacttgcg ctcaccttgc tgatcgtcac gtggtatctg
    52501 aaccgtgcga agcgggtgga ctccatcgag gcggttgtcg gcctgctgag cgtcactctc
    52561 ttcggcattc acagcatgct ggagctgccg tatcactacg cctacttcct gattccgatc
    52621 ggtctgtgga tcggccatgt cgagcacgcc tgcggctctg gtatccggcg gttcgcagct
    52681 tggcgatggc tgcccgcggc ggctgcggcg gtgatggtgt tgggcatatg gcgtgactat
    52741 ccgaagatcg aagaggaagc tcggcgcgtg cgcttcgaga accttcggat cggcctgacg
    52801 accgcggctc agcccacgcc agatgccccg tttctgtcag gcctcacagc attcctcgag
    52861 ttcacgcgca ccaagccgag tgagggaatg actgtcgcgg acctgaaggc catggaaaac
    52921 gtggtgaagc gataccccta tgccggttcg attgcgaagc tctcctgggc ttgggcgctg
    52981 aatgggcgct tgccagaggc gattgagatg ttcaaaaaga ttcgctacgt gcacggagac
    53041 gtgctttatc accgaatccg caatgacatg catcaacggg tggttgatgg gcagattggc
    53101 ctgcgcgctc ttgacgacgc ggtacaagac tgaggcagtc aacctgagcg gtggcatcgc
    53161 gttctggcat gggacgcact atcggtctcg cagcatgggc cccgagaaat ggcttctgac
    53221 tttctaccca tccgcatcaa ggccgcgggg ctccacctgc ttgccagctg tctggttgcc
    53281 gcgctgtcgg cggccctggt gtttcttgtc tggtatccat ctccgtatgc cacgatggct
    53341 ggcggctccg gcctgttcct gttgatcgtc agcattgatg tcatcctggg tccggcgttg
    53401 accgcagtcg tggccaatcc gaacaagaag cgctccgagc tggcacgcga cttggccgtc
    53461 atcgtgactg tccagcttgc agctttcggc tatggcctct acacgatggc actggcgcgg
    53521 ccggcacttc tgacattcga agtcgatcgc cttcgtgtgg tcacggcggt tgatgttgat
    53581 ccagtgttgc tcaaggaggc accccccgga ttgcagtcct tgtcatggac cggaccgcgt
    53641 ctgatcgctg ccgtcaagcc gaccgacccg gatgagcaga tgcgctctat cgagctcggg
    53701 ctggccggca tcgacctggc catgcaaccc aggcagtggc gcgactatgc agcgcgcgcc
    53761 gacgaagcct ggcgcgtggc ccggccggtg tcggtgctgc tgtcgcagta tccgcagctg
    53821 gccgccgacg tggcccgcat cgcgcggtcg gcaggccagc cgaccgagtc cctgcgcttc
    53881 ctgccgctga tgtcgcgcca ggccagctgg gtctcgctga tcgcggcccc cggcgcacgc
    53941 gtcgtgggcc acctgccggt cgaaggcttt ttctgagctg ttgcgggctg gccgcggccc
    54001 ggggcttcgg gcgggatggc cgcattacga tcgccgtctt cgaaaggaac cgagatggca
    54061 gatataccga cgggcaccgc agacccactt cgagttcgcg gcgtatgcgc ctgctgaccg
    54121 cgtgagttcg atgggtcggc gcgagccgaa tccctcgtcg gtgaaacgcg gcccggcctc
    54181 gccgcctggc agcccgtggc tcacgatcgc tgcctgcctg gcggtggttt cgcctgccgt
    54241 gctggcggtc aacctgccgc cgtcggccac ctttctcaac caggcagccg cggtttcggg
    54301 ttggggtctg ttttgtgccg ttctggcatg gagtctggtg cctggaccct cagccccgtc
    54361 gatgcggtgg ggcgtcgggc tgcgggcgct gctggctgcc gtggcgctct tgatggcggc
    54421 ctgcgcgggc tcgtggatga tgaccgggtt gccctcgtcc ctgacgctgt cggcgatcgg
    54481 catgctggcg gcggcggccc tggtggcctg cgttggtgct gccgcgatgt ctgcgggcca
    54541 gggtgcggtg gcgtttcgtg ccttctgcat cgcgctggtg atggcgggca tcctcagtgc
    54601 ggccatcggc gcggttcagg tgttcgctcc gcaaggggcc gatggacact ggatcgccat
    54661 ctcggcgctg gagggccggg cttccggcaa cctgcggcag cccaaccacc tgagcagcgt
    54721 gttgctgtgg tcggtggtcg ccttgctgtg gctcgccgaa tcgatgggca caggctgctt
    54781 atcggcgcga ccggtccggc gggtcggcct gtatctcggg ctgctggctc tcatcttcgg
    54841 actggtgctg agtgcctctc gcacgggaat gctgggcgtg ctggcactcg ccatctgggg
    54901 tgcgctcgat cgcaagctct cgcgacgcac tcgcgtgctg ctgtgtgcgt cgcccttgat
    54961 ctatgccctg tgctgggtgg gcctgacggc ctgggccgat gcgagcgggc atgtcttcgg
    55021 cggggccgcg cggctgcgca gcgaggccga catctcgagc tctcgcttcg gcatctgggc
    55081 cgacacgctg gagctgatcg cgaagcgacc gtggttcggc gtgggctggg gcgagttcaa
    55141 cctggcctgg tcgctgacgc catttccggg gcggccggtt gcgttcttcg atcacacgca
    55201 caacctgctg ctgcaactgg tggtggagct gggcgcgccg ctggccctgc tggtcaccgc
    55261 gctgctggca tggtcgctct ggcgagcctg gaaggccggc tgcacgtcgg tcgccggcgg
    55321 cgatcacccg cctgcggcgc tggtgcggtc tgcctggatg atggtgctgc tcgtggcctg
    55381 gcacagcttg ctcgagtacc cgctgtggta cgcctacttc ctgctgccgg ccgccttcgc
    55441 gtggggcctg tgtctcggcg cggagtcgcc gaccgcgcct tcaccgaagc ccgcatcggc
    55501 tgcgcgtact cccgcctggt cgctttggct cgcgtcgatg gcgatgacgg cggctggcgc
    55561 cgcgtcggtg atcgattacg tcccggtggt ggcgatcttc gagcccggcg acgatgcacc
    55621 gccgctcgcg gagcgcatcg ccgcaggccg gcgcagcgtg ctgttctcgc accatgccga
    55681 ctatgccgcc gccaccaccc gtgccgcgcc tgccgaagtg ctgctcgcga tgcagggcgc
    55741 caagcatttc ctgctcgaca cgcggctcat gaccgcctgg gcgcaggcgc tcgccgacag
    55801 tggcgacatc gaacgggcgc gccacatcgc ggcgcggctt cgcgaattcc gcaacccggc
    55861 atcgcgcgag ttctttgcgc cgtgcgccga tggttcgatg ccgccgccat tccagtgcac
    55921 gccgccgacc accgtgatgg atgagcgtgc cttccgttga cctgtcgcgg tgcgacagaa
    55981 tttctgccgc ttgaatccgc cgggtcccgc gccgactact gaccgctgtc ggctcccgca
    56041 gctgcgttgc cttcaatgga ccacgtgccg ctcttgccgc cctgcttctc cagcacccgg
    56101 atctgtccca tcaccatgcc gcggtcggcg gccttgcaca tgtcgtagat ggtgagcagg
    56161 ccgatctgca ctgcggtcag cgcttccatc tcgactccgg tgcggcccag ggtttcgacc
    56221 tgcgccgtgc agatgacccg gctggtggct ctgtcgatct cgaagtcgac cgcgacacgg
    56281 gtgatcggca acgggtggca cagcgggatc agctcggccg tgcgcttggc gcccaggatg
    56341 gcggcgatgc gcgccacgcc gagcacgtcg cccttcttcg cctggccgcc ttcgatcagc
    56401 gcgagcgtgg tcggcagcat gcggatctcg ccacccgcgc gcgcgatccg gtgcgtttcg
    56461 gccttgccgg ccacgtcgac catgtgcgcc tgacccgtcg catcgaaatg cgtcagtggt
    56521 gacgcttcct cgggcatggg tggcgcgggt tcggcaggtg cggtcatggt gcgaaacaaa
    56581 gcagcaaagt caggctggca tcatagtgag cacgcgcgct gcaacgcgga agaccgccca
    56641 gcggcaagcg atcaatctga actcgttgcc gcgcccgagg tctcaacgct gcaccagttg
    56701 cgaaagtccc cttgactcag acacctgctc atctccgcgc tgctcctgcg accgctcgcc
    56761 cggcagccga ccgtccacgt ctgtcgcagt caccgtcacg ccggatgctg gtgggcctgc
    56821 tctgcgtgtc gttgctgggc ggcacgtggt cgcctcaggc ccaggcccag gtgaacctcc
    56881 ctgcgctcgg cgacaccgca tccgaggact tcaatctcgg caccgagcgc cacctgggcg
    56941 acgcgatcat gcgcgacatc cggcgcgatc ccgattacct cgacgatccg atcctgctcg
    57001 agtacctgca gtcgctctgg caaccgctgc tggctgctgc acgcctgcgc ggcgacctcg
    57061 gcgatgacct gaaccagcgt ttttcgtacc agccgttcct ggtgcgtgac cgcagcgtca
    57121 atgcgttcgc gctgcccggc ggttacatgg gtgtgcacct gggcctgatg gcgatcacgc
    57181 agacccgcga cgaactggcc tcggtgctgg cgcacgagat gacgcacatc aaccagcgtc
    57241 acatcgcgcg cggttttgcc aacagcaaga agcagtctct gatcagcgcc gcggccatga
    57301 tcatcggcgt gctcgcggcc acccgatcgg gaggtggcga tgccgccaac gcgatgatcg
    57361 ccggcggaca ggccgtgggc atccaggggc aattgaactt ctctcgcgag atggaacgcg
    57421 aggcggaccg catcggctcg cagatgatgg cgagcgccgg cttcgagcct tccggcatgg
    57481 cgtcgatgtt cgagcggctg cagcagtcct cgagcctcaa cgacagcggc ggctatccgt
    57541 acctgcgcag ccacccgctg acgaccgagc gcatcggcga ggcccgggcc cggctcggga
    57601 cggcgggcct cacctctgcg ccaccgctgg tcagttcgcc gtcgacggcg ctcgaacacc
    57661 gggcggcgct ggcccgtgcc cgggtgctga tggacacgcg ggtcgatgcg ctgcggcgct
    57721 ggcaggccct cgatggcgaa ggcagcgcgg ccagcgcgac gctgggtgag cgctttgccg
    57781 acgcgtactc cagcgcgctg gcttccagcc tgttgcgcga ctgggtgcgt gccgatgcct
    57841 cgatgtcccg cgcgtcggac atcgctcgcg gcgggtcggc tgccgtggcg tcgatctccg
    57901 acgtggcggc caccggcgcg ctgccgcagc gtgacgcgcg tggcgagcgt tcggtggcac
    57961 tgctcaaggt ggagtctttc ctgcatcgcg gcgagcccgc tcgggcgcag gcgatcctgg
    58021 caccgctgac ccgcagcgtc gggccggtcg ccgcggaggc cgcgaccacc tcgcgtccgg
    58081 tgatggtgct ggcagcgcag gtcgcgctgg ccgaaggacg ctcgaaggag aacgcggcgc
    58141 tgcgcgcgag cgcggatcag ctgcagacct gggtggcacg ctacccggac gatgccgagg
    58201 tctggtcgat gctgggccgg gtgtcggcac agctcggatg ggcactgaga tcggtgcgtg
    58261 ccgaagccga gtcgcgctat gcatcgggcg acctgcccgg ggccatcgac cgcctgaagg
    58321 ccggccagcg cctggctcgc agcagcacca cggcggtcga tttcgtcgag tcctcggtca
    58381 tcgattcacg gctgcgttcg atcgaggcgc accgccgcca gcttctcacg gaacaattgg
    58441 ggtaccagta gacgaagcca tcgaggcgag gtgcacgtgg tacgagcttg gatccggagg
    58501 tgggggcctg cgctgctggc gctggcgtcg ttgacgagca tcccggccga agccgcgacg
    58561 atcgtcactg tcagtccgcg aggcgaagtg gcgcaggtgc ggcagctggc agtcacgttt
    58621 tccgaggcgg tcgtcgcctt cggcgacccg cgcctgcccg atccgatggc ggtgtcctgc
    58681 acgggcgccc tggcccgggg ctcggggctc tggaccggcg atcgccactg ggtcttcaac
    58741 ttcgaagagc cgctgccgcc cggagtgcgc tgcagtgcgc aggtgcgggc cgactggaag
    58801 ccgcgcgtcg cgggcaccga tgctgcgttg accggcacca gctctttcag cttcagcacc
    58861 ggcgggccgg cggtggaatc gatcgagccg tatgacggct cgacgatcga ggaggaccag
    58921 cacttcctga tcaacgtcaa cggtgccgcc accgagcgca gcgtcgcgac ccacgcctgg
    58981 tgcgaggtcg agggactcgg tgaccggctg ccggtgcgga tcgtgaccgg tgcactgcgc
    59041 gagcgcatcg tcgcggccgc cggtccgcgt gcgctgaagg acgcgaagcg cgaggccgag
    59101 cgccagtcgc ggctgatcgt gctgacctgc ccgcggccgc tgccgaccga cacaccgatg
    59161 cgcctggtgt gggggcgtgg catcgcggcg gtggtgaacc ccggcgtggc gacacgcgtc
    59221 gagcagcgtt tcggctaccg cgtgcgtccg gccttcaagg ccgagttcag ctgcgagcgt
    59281 gaacgcgcca ccgcaccctg cctgccgatc cgcccgctcg tgctgcggtt ttctgcgccg
    59341 atcgatcgca agctggccga gcaggtgcgg ctggttccgg ctggcggcaa gccgatcgcg
    59401 ccggtcttcg acaaggacga caaggacccc gaggtcagtt ccatcacgct cgcgatgcgg
    59461 gtggccgaga acgcggccta cacggtcgag ctgccgtcga gcctgaagga tgccgcaggg
    59521 cgcgtgctcg cgaacgccgg cgcgttcccg atgaaggtga gcacgggcga cgcgccgccc
    59581 atcgccaagt tcccggccgc tccgttcggg atcatcgagc gcttcggcga tgcggcgaat
    59641 ccgggcgcgg tatcggcacc gacgccgatg ctgccgctca ccctgcgcca tgtgcagggc
    59701 gatctgcgct cttcggctcc cggcggcgcg gtgcgcgtca agcgcctgca gaccgatgcg
    59761 gagatgctgg cctggtacga caagctgcac cgcttccacg agtccgatct cagtgcccag
    59821 gaggccggcc tgccgaaggc gcagtggttc accaccgagt cgctcgtcga cgagcgcgga
    59881 cgatccaccc ggcagcaggt cgagcgccgt gtgcagacgc gcacgctgtc gctgctgaaa
    59941 gccgacacca gcgcgaagcg actcgatctg ccccagctga agggcggcga cccgcgtccg
    60001 ttcgaggtgg tcggcctgcc gctcgcggag ccgggctatc acgtggtcga gatcgaatcg
    60061 gcgcgcctcg gccagtcgct gctcgagccg cgcgccccga tgtacgtgcg caccggcgtg
    60121 ctggtcacca atctgggcgt gcacgtgaag ctgggccgcg agaacagtgc ggtgtgggtc
    60181 acctcgctcg accgcgggcg gccggtggaa ggcgccgacg tggcgatcaa cgactgcttc
    60241 ggcaagcggc tgtgggccgg tcgaaccgat gcgcgcggcc tggcgatcgt caagctcgcg
    60301 ctcgacctgc aggtgggcga atgcccgaac gaagcgggct acttcgtgac cgcccgcaag
    60361 gccgatgccg acgcgcgcac cccccaggtg atcgacaccg cgttcgtgtt caccagctgg
    60421 cagaagggca tcgaatcctg gcgcttcaac ctgccgacca gcaacgcctc cgagcgcgat
    60481 gccgccgccc acaccgtgtt cgaccgaacg ctgctgcgcg ccggcgagac ggtttcgatg
    60541 aagcatttcc tgcgtgctga aaccttggcg ggtctcgcgc cgtgggatgc cgcgtcgctg
    60601 cccacgcgca tcaagatcgt ccaccagggc agcggccagc agatcgtgca gcctctggtg
    60661 tggaacggca cgcgctacgc ggtgtcgacc tggaacatcc cgccggctgc caagctcggc
    60721 ctgtacgagg tcatgctgga gcgcgatgtc gtcaacgaac gccaccgcag cagctggagc
    60781 agcggcagtt tccgtgtcga ggagtttcgg ctgccgctcg tcgatgcgcg cctgagtggt
    60841 ccgagccagg ttccggtcgc accgcgcgag ctgcccttga gcgtgcagat ggccttcctg
    60901 tcgggtggcg cgatggccaa gtcacctgct gcggtgtcgg cgctgctgaa gccgcgcgtg
    60961 ccggcctttc ccggccacga cgggttcagc ttcgagccac cgcgcgtggc ggctgccgat
    61021 ggcgattccg aagacgaaac tgcctcggca cagggtgcgg atcggttggt cgccgacaag
    61081 ctgccgctga ccaccgatgc gcaaggcgcg gccacggtgt tgctgaagga cctgccgccg
    61141 ctgacgcgcg ccagcgagct gcacagcgag ctgacctaca ccgacccgaa cggcgagatc
    61201 cagacggtgt cgagccgcat cgacctgtgg ccgagcgcgg tggtgctcgg catcaagacg
    61261 cgcgcgtggg ccagccagcg cggcggcagt gtccagttca acgtgctggc gctcgacacg
    61321 cgcggccagc cgattcgcaa ccagcgcgtc gaggtgcgtg gccgcctgtc gcagacactg
    61381 agcaaccgga cccgcatcgt cggcggcttc tacgcctacg acaaccgcac cgaactgaag
    61441 gacctcggcg tggtctgcag tggcagcacc gacgaacgag ggggcctggc gtgcgacgcc
    61501 acgctgtcgg cggccgggca ggtggagctg atcgcgcagg cgaaggacgc ggcgggacgc
    61561 acggcacagg ccgccaccgg cgtgtggatc acgaagcagg gcgaactgtg gttcgggcag
    61621 gacaacgacg accgcatcga cctgctgccc gagaagccgc gctacgagcc cggcgaaacc
    61681 gcgcgtctgc aggtgcgcat gccctaccgc gaggcgacgg cgctggtcac cgtcgagcgc
    61741 gaggggctgc tcgacacccg ggtcgtcacg ctgagcggcg acgacccgac gctgtcgatc
    61801 aagatcgaga agtcgtgggg gcccaatgtg tatgtcggcg tgctggcgct gcgcggccgg
    61861 ctgcgcgagg tgccctggta ttcgttcttc acctggggct ggaaggagcc gctgaactgg
    61921 gcgcgatcgt tctggtacga cggacgcgag taccaggcgc ccaccgcgat ggtcgacctg
    61981 gccaagccgt cgttcaagtt cggcgtcgcg gcgctgcagg tcggcatcgc agcccatgaa
    62041 ctgagggtca gcgtgacgac cgaccgtgcg cagtacgcgg tgcgacagaa ggtccaggcg
    62101 cgcatcaagg tgacgcagtc ggccaatggc gagaggcccg ctccggccgg taccgaggtc
    62161 gcgttcgcgg cggtcgacga gagcctgctc gagctgcgcc cgaacgagtc gtgggacctg
    62221 ctgaacggca tgatccgcag ccgcgcctgg ggcgtcgaga ccagcaccgc gcagggcgag
    62281 atcatcggca ggcgccacta cggtcgcaag gcggtggcgg ccggcggtgg cggcggtcga
    62341 ggcggtacgc gagaactgtt cgacacgctg ctgctgtgga aggcggtggt cgtgctcgat
    62401 gcccggggcg aggccacggt cgaggtgccg ctgaacgact cgctgacgcg cttccgtctg
    62461 gtcgccatcg ccgacgggcc gctcgacgcc gccacgccga ccttcggcac cggcagcacc
    62521 acggtgcgcg tgacgcagga tctgcagttg ctggccgggc tgccaccgct ggtgcgcggc
    62581 ggggatcggt ttggcgcgtt gctgacgttg cgcaacacga ccgcgcacga aatgaaggtt
    62641 cgcgcgacgc tggccggcac ggcgaaccgc ggcacggccg agatcgtgcg gacgccgctg
    62701 gcgttcgcac cgcaggacgt ggtgttgccg gccgggtcgg cgaaggaact cgcctggccg
    62761 gtcgaggtgc cgatcgatgc gttcagcatc gtctgggatg ctgcggtgga gaccgttgcc
    62821 gccgcgggcg ctgcgccggc gaaggaccgc atcaggctca cgcagctcgt cgccgaagcc
    62881 gtgccggtgc gggtgatgca ggccacgatc gcgcggctcg acggcccgta ctcgctgccg
    62941 ctcgcggcac cggcggatgc gctgcccgag agcggcgtca agcgcggtgg cctgcgagtc
    63001 tcgctgcagc cccggctcgg cagcgcgctg cccgggctgc ggcgctactt cgagacctac
    63061 ccctactcct gcctcgaaca gaaggccgcc aaggcgctgg ggctcggtga tgcggctctc
    63121 tggggcactc tggtcgagac gctgccgacc tacctcgacg ccgacggact cgccggctac
    63181 ttccctccgc gtgcgggcga tgcgtcggcc ggcaacgacc ggctgaccgc gcagctgatc
    63241 gcgacgtcgc acgaggccgg cttcgcgctg cccgatgcgg cacgcaatgc gatgctggag
    63301 ggcctctcgg cctttgtcga aggccgcatc gagcgccggc tgtgggcgcc tgcgtcggcg
    63361 cagggcctga acctcgcggt gcgcaagctc gcggcgctgg atgcgctcgc gcgccacggc
    63421 cgcgtgcagg cacgcatgct gggctcgatc aacgcgacgc ccatgttgtg gccgaccgcg
    63481 gcggtgatcg actggctcaa tctgctgcgg cgcctgcgtg cgcaggacat cgccgtgccc
    63541 gatcacgcca ggcgcatcga gcaggcgcag cagatcctgc gcagccgcct tgcgctgggg
    63601 ggcacgacgc tgaagttcag cgacgaggtc ggcgaccact ggtggtggct gatggacagc
    63661 gccgatgcga acgcggcgcg gctgatcctg gcggtgctcg acgagccggc ctggcgcgac
    63721 gacctgccgc gtctggtggt cggcgcgctg ggccgtcagc gagacggtgc gtggtcgacc
    63781 accaccgcca acctgtgggg tgcgatcgcg ctcgacaagt tctcggcgaa gttcgagtcg
    63841 caggcgccga cgggatcgag cgcggtgcgc gtcgattcca ggccttcggg cgcgtcggct
    63901 gctgctgcgc cgccagcgct ggcatcgggc atcgtcgact gggcgaagca gcctgcgggt
    63961 ggctcggtga tgcttacatg gcctgccgaa ccggccacgc tgtcggtccg tcagcagggc
    64021 agcggcacgc cgtggctgac gttgcagggc ctcgcggcaa tcgcgccgaa aggtccagtg
    64081 cgcgccggct acggcctcac gcggcacatc acggcggtat ctcgcaagga cccttcgcgc
    64141 tggtcgcgcg gcgatgtgct gcgcgtgcga ctggaaatcg atgcgcagac cgacatgagc
    64201 tgggtcgtcg tcagcgatcc ggtgccaggc ggcgcgacgc tgctgggctc ggggctcggt
    64261 cgcgacagcg cgatcgcggc gcagagccga tcgggcgagt cggacaccga cgccggcagc
    64321 gcctggacgg cctatgacga gcgcagcttc gaggcctttc ggcgctacta cggccagttg
    64381 ccgcgtggtc ggcatgtcgt cgagtacacc ttgcgcctga acgctgccgg ccgctttgcg
    64441 ctgccgccga cacgcatcga ggcgatgtac gcgcccgaga ccttcggcga gctgccgaac
    64501 ggcgccatcg acgtggcacc gtgaagcgca tcgtctgggc ggctgcgctg ctgctggccc
    64561 tcgaagccgt cgccgtcgtc ccgcgcttcg aggacgtgcg cgcgcggcac tcgccatccg
    64621 acaccacgct gctcgaccgc cagggcgtgc cgatccagac cctgcgcacc gacagctcgg
    64681 tgcggcgcct gccgtggctg ccgctgagcg agatgtcgcc ggccctgttg caggccatcg
    64741 tgttcagcga agaccgccat ttccacgaac acagcggtgt cgattgggcc gccttggcga
    64801 gcagtgcctg ggccaacgcg tggaacaccc gaacgcgcgg cgcatcgacg ctgaccatgc
    64861 aactggctgg cctgctcgac gcgcggctgg agcggccggt cggtggacgc agcatgacgc
    64921 agaagctcgg ccagatcgtg cgcgcgcgag agctcgaagc gcgctggagc aaggcgcaga
    64981 tcctcgaggc ctacctgaac agcgtggcct ggcgcggcga gctggtgggc atcaacgcgc
    65041 tgtcgcagac gctgttcggc aagcgcgcca gcgggctcga cacgcacgag gccgccgtgg
    65101 cggcagcgct ggtgcgcggc ccgaacgcga gcgcagcggt cgtgacgcag cgcagctgtg
    65161 gcgtgctgca ggctcagggc atgagctgtg caggactgtc cgcgctcgtc gagctcgcgc
    65221 tggtgcgacg cggcgggccg gtcctcggag agcagctggc gccgcatgct gcgcggtacg
    65281 cgctggcgtc cgtcaggaag ccgccggcga cgctgcgaac cacgctcgac gcgtccaccc
    65341 agcgggtggc gaacgcggcg ctgcgccagc agctcgtgga gctggcgggt cgacaggtcg
    65401 aggacggcgc ggtgctggtg ctcgacaacg ccagcggcga ggtgctggcc tgggtcggct
    65461 ccagcggcga gctgtccgac gcagcccagg tcgacggcgt gctggcgcgg cgccagccgg
    65521 gatcgacgct gaagcccttc gtctacggcc tcgcgttcga gcggcgactg atcacgccgg
    65581 cgagcctgct cgacgactcg cccacgcagc tcgccacggc caccggcctg tacctgccgc
    65641 agaactacga ccgcgctttc aagggctggg tcagcgcgcg caccgcgctc gcggccagcc
    65701 tcaatgttcc ggcggtgcgc gtgggcgcga tgctgggtgc cgatgcactg tgggcacggc
    65761 tcaacgcctt cggattcgag ctgccggaaa gcggcggcta ctacggggac tcgctggcgc
    65821 tcggcagtgc cgacgtgacc ttgctggcac tcacgaatgc gtatcgcgcg ctggccaacg
    65881 gcggcatgct gtctccggtg atgctgcggc ggtcgtcgac cacgtcaccg gacgcatccg
    65941 ttgcgccgcg cgtcgcatcg aagcgcgtgg cgaacgcggc ggccacgcac ctcgtcaccg
    66001 acatcctgtc cgacaacaac gcgcgcgccc tgaccttcgg gctcgacagc gcgctcgcgt
    66061 tgcgcggtcc tgttgcggtg aagaccggca ccagcaagga cctgcgcgac aactggtgca
    66121 tcggctactc cgatcgctac acggtcggtg tgtgggttgg caacgcgagc ggtgcgccga
    66181 tgcacgacgt gagcggcgtc agcggtgctg cgcctgtctg gcgcgccgtg atgcagcatc
    66241 tgcacgaagg cgtgccggcg aagcagagag cggtgcccgc aggtgtcgtc atcgctcgca
    66301 cacgcttcga gggtgtgcgt gaaccggcgc gcgacgagtt gttcatggcc gggaccgagg
    66361 tgacccgacc gaggccggac atgcagggcg caatcgggat gacgagggcg gcgagcgcct
    66421 ttggcatccg ctcaccgcgt gagggcagcc tgtacgcgat cgatcccgac atgccgccgc
    66481 gtgcccaacg gatcactttc gagggcgaag cgggcacctg ggtgctggac ggccaggtgg
    66541 tggggcgggg gcagcggctg cactggacac cctggcccgg ccgccaccag ttgacgctgc
    66601 gcgggcgcga gggtcaaacg ctgcaggccg tgcgcttcga ggtgcgcggc gccgccgtca
    66661 agtcggcatc ggccgcgcgt tgagcgaggt caggacttcg gggaaaagat ccgctccatc
    66721 gcgacgtcga tcaccatccc gcacaggctg tagatcagcg agccgatcag tgcagccgcg
    66781 aagccggtga cgttgaagcc cgagagcagg ttcgcggcga aatagaacat cagcgcgttg
    66841 atcacgaaca ggaacagccc cagcgtgacg agcgtgaccg gcaaggtcag cagcaccagc
    66901 acgggccgca gcagcgtgtt gagcaggccg agcacgaagg ccgcgagcat ggccgaaccg
    66961 aagctctgaa cggagacgcc gggatacaga taggccacca gcagcaaggc ggcggcgaga
    67021 aggagccagc gaacgatgat cttcatgcga gaagaataac cccgcgccag gccgcacgcg
    67081 atgaacttgc cgcgtgctcg aggccgccgg cgaagggccc ggcgatgctg tcggtggtgt
    67141 gttcgcgacc tcgacgaacc gcagtgcgga ggtcgcgaat cccgtcgatg gcacgcccga
    67201 acgcgttcca ctccgtgacg cagacacgac aaaccgctcg ccaggccgca agaatgcgtg
    67261 tttctcctgt gaaacgcgct tctcaatgcc cggggcacgc agtacgatcg gcagcgcacg
    67321 aaagctgtga tgacgagggc tcatgcggtt ctccaccagc ccggcacatc aaccacttat
    67381 ttgatggaga gaattgaaat ggcaactgcg aagaaggctc cggccaaagc ggctccggcg
    67441 aagaaggcgg ctcctgcaaa gaaagccgcc gcgaagacgg tgacggccaa gtcggcggcc
    67501 accaagaccg cagcgcccgc caagaagcgc tcgccgaacg ccgcgttcat gaaggcggtc
    67561 acgccgagtc cgcaactggc agcgatcatc ggctcgacgc cgatgccacg caccgaagtg
    67621 acgaagaagc tctgggagta catcaaggcg aacaagcttc aggacgcgac caagaagacc
    67681 aacatcaacg ccgacgccaa gctgaaggaa atcttcaaga aggcccaggt ctcgatgttc
    67741 gagatga
//
LOCUS       DAOORV010000004        67269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_436_length_67269_cov_8.088925, whole genome shotgun sequence.
ACCESSION   DAOORV010000004 DAOORV010000000
VERSION     DAOORV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 67269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_436_length_67269_cov_8.088925"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKV56_01000"
     CDS             complement(<1..841)
                     /locus_tag="PKV56_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX66410.1"
                     /translation="MATTTGNNAKGAAAAPRPMTGEEKKVIFASSLGTVFEWYDFYLY
                     GSLAAIIAKQFFSGLDANSAFIFALLAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVT
                     ILIMGLSTFIVGVLPNYASIGVAAPVILIGLRMLQGLALGGEYGGAATYVAEHAPHGK
                     RGAYTSWIQTTATLGLFLSLMVILGTRTMLGEEVFADWGWRVPFLVSIFLLAISVWIR
                     LSMNESPAFKKMKEEGKTSKAPLTESFGQWKNLKIVILALVGLTAGQAVVWYTGQFYA
                     LFFL"
     gene            complement(1057..1281)
                     /locus_tag="PKV56_01005"
     CDS             complement(1057..1281)
                     /locus_tag="PKV56_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOX66411.1"
                     /translation="MNQPPLPHAGSSVPSPCVGVCCLDDTAGWCTGCLRTRDEITDWG
                     KLCDRDKQVIWKRLTQRRRELPVRPPLPDT"
     gene            complement(1299..1913)
                     /locus_tag="PKV56_01010"
     CDS             complement(1299..1913)
                     /locus_tag="PKV56_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOX66412.1"
                     /translation="MPPLDTTQLLAIAAALGWASGLRLYAVVFLTGLSGVLGWVDLPT
                     GLKVLQSPLMLGASGFMLFIEFFVDKIPGLDTLWDIVHTLVRIPAGAALAAAVFGADH
                     TSWAMAAALMGGTLAATSHIAKATTRAAVNTSPEPFSNIGMSLLGDALVPTALWLSWT
                     HPLAFAALLLACLVAMVGLIWALGRFLRQLATTITAWRPPVRRS"
     gene            complement(1916..2551)
                     /locus_tag="PKV56_01015"
     CDS             complement(1916..2551)
                     /locus_tag="PKV56_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOX66413.1"
                     /translation="MSLEPRPIEFYFDLSSPYAYMASEWIDTLAAQHGRSVNWNAILL
                     GATFQAAELRSPVAHPLKREYSLRDFERSARFSAVPFVLPSRFPVATQNAARVYWWLR
                     DQSAGGPDPAHLLDARGWAKHCFRALFVQGADLSDVARLREIAVEFGLDAAQAEAVWN
                     DPRWKARLKEVNDMAISAGVFGAPFFVIDGEPFWGNDRRAQIERWLASGPF"
     gene            complement(2548..3135)
                     /locus_tag="PKV56_01020"
     CDS             complement(2548..3135)
                     /locus_tag="PKV56_01020"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HOX66414.1"
                     /translation="MRTAPLDRALRGWLQSDGSLSRRLARVFGRFEVQVLSQGAGPAQ
                     ADELCVLRRSGGVALSAARCHVREVMLWGNGQPLVYARSVLPAVQSRLAWRALRGLGS
                     RPLADLLFGLRAASCARLGSSRMVPLRARRLGARLGWAREPLWCRRSVFMRRGMPLLV
                     TEWFAPSVCQCTPGAPTGRGTFRGFRGEPMSRSLR"
     gene            complement(3151..4932)
                     /gene="aceK"
                     /locus_tag="PKV56_01025"
     CDS             complement(3151..4932)
                     /gene="aceK"
                     /locus_tag="PKV56_01025"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOX66415.1"
                     /translation="MNFPPTLTDVRSFDIARAMLDGFNRHYRLFREASRQAKRRFEEA
                     DWHGQQRGQRERIEYYDARVDEAAERLQTEFKAAGLSSEVWQQVKLHYIGLLTNHHQP
                     ELAETFFNSVTTKILHRSYFNNDFIFLRPAVSTEYIENDEPASTPSYFAYYPTKATLR
                     DTWRRIVDHFELRRPFENLDRDIDDVLAAVEKQLGDYRPRANFQIQVLSSLFFRNKGA
                     YLVGKVINGYRETPFALPILHVAGGKLAIDAVLFGEEDLLLLFSFARAYFMVEMEVPS
                     AFVQFLRTMMPRKPRNEIYSALGLQKQGKNLFYRDFLAHLRHSSDQFRIAPGIKGMVM
                     LVFDLPSFPYVFKVIKDYYPPQKETTRELIKSKYLLVKQHDRVGRMADTLEYSNVAFP
                     RARFDDELVDELKKFCPSLLEEDGDDLVIRHVYIERRMIPLNIYIQEASPEQISQAVV
                     EYGNAIKDLVAANIFPGDMLWKNFGVMRHGKVVFYDYDEIEYITDCNFRRVPAPRNEE
                     EEMSGEVWYSVGPKDVFPETFGPFLLGNAKVREVFMKHHADLLDAAFWQEHKERILAG
                     HVHDVFPYEAAKRFEHQRHAGAGVAAS"
     gene            complement(4947..5642)
                     /gene="can"
                     /locus_tag="PKV56_01030"
     CDS             complement(4947..5642)
                     /gene="can"
                     /locus_tag="PKV56_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOX66416.1"
                     /translation="MTGDLKELFENNRMWAEATEARTPGFFTNLLKQQAPQYLWIGCA
                     DSRVPANELVGLLPGELFVHRNVANVVAHSDLNCLSVVQFATDMLRVRHIIIVGHSGC
                     GGVAAALHNRRVGIADNWIRYVQDVRNQHEEWLETVDAAQRVDALCELNVIEQARNLC
                     HTTMVQDAWNRGQELVVHGWFYGLHNGLLEDLKMTVSRPEQVRDAYQGALAALRARRT
                     PSAASGDSPVEGT"
     gene            5703..6836
                     /locus_tag="PKV56_01035"
     CDS             5703..6836
                     /locus_tag="PKV56_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX66417.1"
                     /translation="MKKSPSTHAAPQEPDYPWGNAVPQAGDSIEVGPGVRWLRMPLPF
                     ALDHINLWLLRDELDVTDGSVSGWTTVDCGMADECTRSAWEQVFATQLHGLPVLRVIA
                     THMHPDHVGLAHWLTQRWSRPASPAGEAFDCRLWMSATEYAAARLGSRSDNWTGGEAA
                     AAFMTAHGLTDAASIELIRTRAGYYASMVPQVPAAYRRLMDGSVLSIGGVQAAGRHDW
                     CCIAGYGHSPEHLALHCASLGLLISGDMLLPRISTNISVGHDEPEGNPLAAFLASLER
                     MRTLPEDTLVLPSHGKPFRGLQSRIDQLQAHHAARLSDVMSACAGSPRSAADLLTVLF
                     RRPLDVHQTTFAMGESIAHLNALWHDGKLVRRLRDGIYRFSPA"
     gene            complement(6886..7809)
                     /locus_tag="PKV56_01040"
     CDS             complement(6886..7809)
                     /locus_tag="PKV56_01040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOX66418.1"
                     /translation="MARPPHRPDPAQLSLFVAQEPTASTAMRSDEVAFDAPATAAKSD
                     PLAAAVFLHPQGSREIVLGGHTVGYALKRVRRRSIGFIVGTDGLSVNAPNWVGIRDIE
                     SALREKASWILGKLHEQQQRGRRLQSARVDWRDGATIPFLGQTVVVVLDPRAGITLSG
                     AVLDASSGDALDAGCAPPRRLHIGLPPGALPEQIRDAVQSWLQRQAKRVFAERCDAYA
                     ARLGVSYRRLSLSSAQTRWGSASADGSIRLSWRLIHFGLPTIDYVVAHELAHLREMNH
                     SPRFWDVVRSVVPDFERTRRALKTDVLPVFG"
     gene            complement(7821..8642)
                     /locus_tag="PKV56_01045"
     CDS             complement(7821..8642)
                     /locus_tag="PKV56_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX66419.1"
                     /translation="MQRMFWAVRSFLFLLYMAVSVVPWATVVLIVSIFARGDRVYWLC
                     AGWLTHSISAARVICGVRHRMHGLDNLAEANRAPAVIVLPKHQSTWETFAFPGLMPHP
                     LAYVFKRELLYIPFFGWAMARMDMIHIDRARRSEAWRRVAEQGARHMKHGAWVIMFPE
                     GTRAERGGQCEYKSGGTRLAVTTGAAVLPIAVTSARCWPRKTFLLRPGVIDVSVGPLI
                     PSTGRRPDELMREVESWIEGEMRRLDPEAYAHESAAPPGLTEVELPAAGRAAPGP"
     gene            complement(8642..9265)
                     /gene="gmhB"
                     /locus_tag="PKV56_01050"
     CDS             complement(8642..9265)
                     /gene="gmhB"
                     /locus_tag="PKV56_01050"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOX66420.1"
                     /translation="MPAASLKLVILDRDGTINEDRDDYVKSPDEWVPLPGSLEAISRL
                     NHAGWHTVLATNQSGLGRGLFDMASMNAIQLKLNQALAQQGGRIDAVFFCPHAPDDGC
                     DCRKPLPGMMHQIAERFGVSLDNTPMVGDNLRDMQAGAAAGCPTHLVLTGKSAGLEGK
                     ALAELLDAVPGTCVHNDLSAFADWLIRQERSHRADTGESDSGFGELH"
     gene            complement(9267..11420)
                     /gene="glyS"
                     /locus_tag="PKV56_01055"
     CDS             complement(9267..11420)
                     /gene="glyS"
                     /locus_tag="PKV56_01055"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOX66421.1"
                     /translation="MTTTANLLIELFVEELPPKALKKLGESFAQVLADSLRAQGLASG
                     HSAVTPFASPRRLAAHVSAVLSVAPDKAACQKLMPVSVALSADGTPTPALLKKLASLG
                     ADESVVPQLKRQMDGKAEALFYDSMVPGITLAQGLQKALEETLSRLPIPKVMTYQLEG
                     GAQPGWTSVQFVRPAHGLVALHGAEVVPLSVLGLNSGRETHGHRFEAAVDPVVLQHAD
                     RYAEQLLEQGAVMAGFAQRHGEIARQLQHAASLQGLTPIDDDALLDEVTALVERPNVL
                     LGRFEESFLDVPQECLILTMKANQKYFPLLDAAGKLTNQFLIVSNISPSDPSAVIGGN
                     ERVVRPRLADAKFFFDQDRKRTLQSRVEGLSKVVYHGKLGTQGERAERVRAIGHAIVN
                     QLSMATLPFTVDAQDEFAVLDSKVQQSALLAKTDLLTDMVGEFPELQGVMGGYYARHE
                     GLRDGVAIAIEDHYKPRFAGDALPRNHTGTVLALADKLETLVGLFGIGQLPTGDKDPF
                     ALRRHALGVIRILTEKNLPLDLPALLQAALPAFGDLLTDPTEALLGFFADRLAVSLRD
                     QGYSAQEVDAVLALAPTRLGDVPRRLAAVRAFAELPEAPALAAANKRVSNILKKSLGA
                     DAHVNPALLKEPAEQALHAAMLQVVPAADAQFAAGDHTGSLQALAALRAPVDAFFDGV
                     MVNAEEIDLRLNRLGLLAQLHAAMNRIADLSKLAS"
     gene            complement(11425..12369)
                     /gene="glyQ"
                     /locus_tag="PKV56_01060"
     CDS             complement(11425..12369)
                     /gene="glyQ"
                     /locus_tag="PKV56_01060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOX66422.1"
                     /translation="MLTFQQIILRLQQYWDAQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGNNPNRLQHYYQYQVVLKPAPSNILELYLGSLEALGF
                     DLKVNDVRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCRPITGEITY
                     GLERLAMYLQGVDNVYDLIWTMGADGTGISYGDVYLQNEQEQSAYNFEHSNVEFLLSA
                     FTAHEGNARHLMEQQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLARA
                     VAASYLDSRARLGFPMAPRAWADEVIAAMAQQRDKKAA"
     gene            complement(12439..14013)
                     /gene="lnt"
                     /locus_tag="PKV56_01065"
     CDS             complement(12439..14013)
                     /gene="lnt"
                     /locus_tag="PKV56_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOX66423.1"
                     /translation="MTRWLAGAPAALLAGVAHTLSFSPFEWWWLQLLSLGVLAHATSR
                     AAPRRAAWLGWLFAVGWLVSGFWWLYISMHQFGGLNPVLAALAVVVLAALLGLYYAAA
                     MALWARLRTGRIAWDLLLWASCWLGAELARGLVFTGFPWIASGYAHAVGPLAAWAPWI
                     GVYGLCALAAALAAVLSMWLSDASVRMRLVTSAGAVAVVLMAHGLPDRFTESTGTLRI
                     SLLQPNVSQDFKFDPERLEAQLRGLVRQLSAARGELVVTPESVLPLSRAQLSDGYWDT
                     LRATLDTPVQPAAAPAGIAAVGSRRAALIGLFVGDDESGYVNSVVAVPASEPTVSGAE
                     YRYGKRHLLPFGEIIPPGFRWFVDLMNIPLVDQARGRSQVAFAFGGQRLRPLICYEDL
                     FGEDIVESVVGPQAATVFVNVSNLAWFGRLLVQDQHLQFSQMRAIEFERPVIRSTNTG
                     ATAVVDHRGKVTARLTSSTEGILEATVEGRVGATPYARWLAALGLWPLILLVLAAIGA
                     SMAVGRGSRGRQPPRP"
     gene            complement(14043..14951)
                     /locus_tag="PKV56_01070"
     CDS             complement(14043..14951)
                     /locus_tag="PKV56_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOX66424.1"
                     /translation="MSDPHPAQPASSGRNRNSPRRTAVTDKRNLFQRLIEFMSPGVDS
                     RDELIEALADAEQRELIAPESRMMLEGVIRMADMTAGDVMVAAPHMDHLDIDAPYDEL
                     LHLVIGTAHSRFPVYEGQRDNVIGILMAKDLLKLQRSPDLNLRTLLRPAVFVPESKGL
                     NELLRDFRSNRNHLAIVIDEFGNTAGLITIEDVLEEIVGEIEDEFDDKDGESSVYTLA
                     DGSHRVAGDAEITAVNEAFGSELPHDTFDTIGGLIAHEMGRVPRRGEAVSLGGLLFSV
                     MLTRGGAVRWFKVTRAPEETFESDGA"
     gene            15077..16111
                     /locus_tag="PKV56_01075"
     CDS             15077..16111
                     /locus_tag="PKV56_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX66425.1"
                     /translation="MALIPATILTGFLGSGKTTLLKRVLTEAHGQKIAVIENEFGDEN
                     IDNEILVRDTGEQIIQLNNGCVCCSIREDLRTTLSDLAEKKRKGELVFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAESYLLDSILTLVDAKHGDQQLDTRQEARRQIGFADQIFISK
                     TDLVDAADVDALAHRIKHMNPRAPQSRVNFGEVPISSVFDLRGFNLNAKLDIDPDFLN
                     DAAHDHDHHDHGEHCDHPSHATHHHAHDDDVKSFVFRSDRAFNPAKLEDFLGSIVQVY
                     GPKMLRYKGVLNMKGSPKKVIFQGVHQLMGSDLGPAWAPGEKKTSKMVFIGIDLPRDI
                     LMQGLEQCLV"
     gene            16502..17221
                     /gene="dksA"
                     /locus_tag="PKV56_01080"
     CDS             16502..17221
                     /gene="dksA"
                     /locus_tag="PKV56_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOX66426.1"
                     /translation="MLKTPLKAADKEAASVPAPAPVAAVAEATVAPAKAPRAKSAAKS
                     RYGAPMPAAPLPPPTSRFADRFAPPRPPTRQMNNLGVDTPRVAPKADPKLANAWKTKA
                     GKDLTEAEILAMPDSEYMNEKQLEFFRATLKKLKDDLLSNAGETTEHLREDTSIVPDP
                     ADRATIEEEHALELRTRDRERKLLKKIAQSLNRVESGEYGFCDETGEPIGLGRLIARP
                     TATLSLEAQQRRELKQKMFGD"
     gene            17255..18910
                     /locus_tag="PKV56_01085"
     CDS             17255..18910
                     /locus_tag="PKV56_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX66427.1"
                     /translation="MADPKDTTSFFRKVVKFVANPSTEWNELGSGSAGSIENDLAKSE
                     LKAMIERKRRNDFVRKREFDMLRKVRREGLTPDQLAALGGSSAMSDSLPRLPDSSGRG
                     SGMKAKIDEIEQQMVGNSRLGTVRRPDEFYSAPTEAVPLDDAASRRASPPAAAAKAGP
                     EPVPSDHSPLTFSVREDASVFDNPFAAAAVNEIAHDPELDEAVIAFANADFDLCEQSL
                     TQLTHANGPRAQHAETWLALFDMHRATGQLPKFESLALEYAQQFGWSAPQWFSMPERV
                     AEAVSEERQPRGRIDGQVGWTSPEWLDTDAVAQLRSQTLQMPLPWVFDWGALRGIDAE
                     AAARLSEVFRLWMPQRLDMRWLNGNRLLSVVQEAAPTGVRDADPVYWQVRLDVLRMTN
                     RPDQFDEAAIDYCVTYEMSPPSWERSQCQVRLSGSGNSTLSHSMSAVSDVSTGFVESQ
                     ILDDAVMVQVSTVELSGQLVGDISELLRNMTEQLGKAPLINVTCARLIRVDFIAAGDL
                     LNWVLARRAENRSVTFVEAHRLVALFFGAMGINEHATVKVRTV"
     gene            18978..19532
                     /gene="hslV"
                     /locus_tag="PKV56_01090"
     CDS             18978..19532
                     /gene="hslV"
                     /locus_tag="PKV56_01090"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOX66428.1"
                     /translation="MDNSNSPPSFHGTTIVSVRRGAQVALGGDGQVTLGHIVIKSSAR
                     KVRKLYRDQVLAGFAGATADAFTLFERFESKLEKHQGHLVRAAIELTRDWRTDRVLRR
                     LEAMLAVADSTASLIITGNGDVLEPEHGIVAIGSGGAYAQAAARALIAHTELSPHDVV
                     KKSLEIAGDICIYTNQSHTIETLG"
     gene            19529..20860
                     /gene="hslU"
                     /locus_tag="PKV56_01095"
     CDS             19529..20860
                     /gene="hslU"
                     /locus_tag="PKV56_01095"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOX66429.1"
                     /translation="MSSNMTPQEIVSELDRHIVGQHDAKRAVAIALRNRWRRQQVDEP
                     LRSEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIIR
                     DLVETAIKQQRETAMRRQRTRAEDAAEDRILDILVPPPRSEAASAAASADNTARQIMR
                     KRLREGALDDKDIEIDLAEAKPTLEIMGPAGMEDMAEQLKGLFANVGGQKRKTRKLKI
                     GDARQLLVDEEAAKLVNEDDIKTAALLQTEQNGIVFIDEIDKVASRNETGGADVSRQG
                     VQRDLLPLVEGTAVTTKYGIVKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELAS
                     LSVDDFEAILTQTHASLVKQYQALLATEGVTLEIAADGIRRLAQIAHDVNERTENIGA
                     RRLSTVMERLLDDVSFDAPNRSGQTITIGAAEVDAKLAELARNEDLSRYIL"
     gene            complement(20887..21729)
                     /locus_tag="PKV56_01100"
     CDS             complement(20887..21729)
                     /locus_tag="PKV56_01100"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOX66430.1"
                     /translation="MSFLAVDVGNTRLKWALYSAAEPGAELLMQGAVFLETVDRLAET
                     DWKLLPRPSSMLGCVVAGEGVKRRVSEQIEDLWHLEPRWVVSTSRAAGVSNGYDHPYR
                     LGSDRWVALIAARQRVLASGAPRAALVVMVGTAVTVDALDAEGRFIGGLILPGFGLML
                     RALEMGTAGLKAPTGEVVDFPTNTSDALMSGGAHAIAGAVERMHRRLLELSGHAPMLL
                     MAGGAAVKLTPITDLPFETVDTLLFEGLLTLQSIKVSPPVASLLPPRSATVGPGDPGF
                     GVAD"
     gene            21897..22730
                     /locus_tag="PKV56_01105"
     CDS             21897..22730
                     /locus_tag="PKV56_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--nucleotide phosphotransferase"
                     /protein_id="HOX66431.1"
                     /translation="MPASDNALGEYRFDGAGKGKPFNLKRIDPSDKPCSSGDKAADRS
                     AVTDLALEIDALQNLLYANRKRRLLVVLQGLDTSGKDGTVRGVFSATSPLGVHAVGWK
                     APTEDELAHDYLWRIHQRMPAAGEIIVFNRSHYEDVLVPVVNGWITPEQTKQRYAHIN
                     DFERMLVETGTVVLKFMLHVSKEEQRVRLQERIDDPTKHWKFSIGDLEVRKRWDDYQK
                     AYADAISATGTTWAPWTVVPADSKTHRNLMVATLVTRALRELDLQPPPADPAISKLKV
                     E"
     gene            22727..23503
                     /locus_tag="PKV56_01110"
     CDS             22727..23503
                     /locus_tag="PKV56_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOX66432.1"
                     /translation="MSLADALDAVDRYRERVRAQAQLARDIQHLQEVARMLFEDKPDK
                     TKAVEAVNDIAAKRLARMDEGARQLLDSEPPIKNWNGSHDRGELIRWLMLVNRPGQFP
                     YTQGIAEPGAPKGDAPHAASRARVTLGAPPDESTGSDHALYLAAALKDGWALLEATKR
                     DSATAGLAELASQIAIAFDEHSCARQCALARAARRIWAVSLREHFGVRGDSLKLKLVS
                     TRSSQGVNPSELEISDRATDQAEDTLLAALQRLVKGSARS"
     gene            complement(23481..23807)
                     /locus_tag="PKV56_01115"
     CDS             complement(23481..23807)
                     /locus_tag="PKV56_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX66433.1"
                     /translation="MSGEPVDRFAVVIEPQGWQFEARGGVSLVESARTAGYVLPTSCR
                     NGTCRACLCRVLAGTVRHLIEWPGLSAEEKREGCILPCVATPETALRIEAPAATRTFV
                     NSLPNP"
     gene            complement(23804..25591)
                     /locus_tag="PKV56_01120"
     CDS             complement(23804..25591)
                     /locus_tag="PKV56_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOX66434.1"
                     /translation="MSDLRSARVGVMRAVALGLAALASSCALLAADRSAMADEPWALH
                     GQNLAGQRHSPLERINAANVGRLVPKWTFHSGVSATFQATPIVVDRVMFVSLPFSHVV
                     ALDAATGQELWRYKHVMRWKQLCCGPASRGVAVSGGKVYLGSADGRLVALDQKTGSKL
                     WDVSVAEYAGSTEAPSQLRAEDTMSRVAATGSTGVGIGAAPLVHDGRVYIGISGVGYG
                     LHPDQGLSVVGVSGQYGQPGLMAAFDAQTGRALWRFDITGPGWEGSFRDATTDGLTTH
                     RDITDEKARAAAHADAWKFGGGAIYVTPVVDVERDLLIFGTGNPSPNMADASRPGDNL
                     YTSSLVALDARSGKLRWYQQQVPHDRWGYDVASPPVLFDLDVDGQRLPALASPSKLGW
                     VYVHDRRDGRFLYKSDAFVPQRNLFTPPQPGDGVLVAPGIAGGANWSPSAYDASLGLI
                     FVPATHLPTRYIAHEVTRPDGSVLQYASTENSDERGGTLSALDLRQAGRLRWQVKIDE
                     PLIGGVLSTASGLIFSGIGHGVFAAIDSASGKQLWSHACEAGVNAPPITYSIGGRQFV
                     AVAAGGNSLFGLKTGDSVVVFGLPDDPAK"
     gene            complement(25600..26004)
                     /locus_tag="PKV56_01125"
     CDS             complement(25600..26004)
                     /locus_tag="PKV56_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66435.1"
                     /translation="MAAVWLQALKVIPWGTVIDAAPGLAKSARGFFKRTQDAARTAAD
                     GATAPSSEAEAGDPLAAAHRRIGALESSLAQATERQQAAAELLDALAAQNAQLVEAID
                     AVRRRVQILVFAVAVLAVGCAGALIWVASIAS"
     gene            complement(26024..26617)
                     /locus_tag="PKV56_01130"
     CDS             complement(26024..26617)
                     /locus_tag="PKV56_01130"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOX66436.1"
                     /translation="MSSPDPAEVSDVPDERAALRVKLIAARQALPDRLERAVQLQSVL
                     RVWLVHRKETAIGGYWPIKGEFDPLPALYRWAEGSDERRIGLPVVDREAGALKFHVWY
                     PGCPMEPDAYDIPKPKGTEVFDPQMVLLPCVGYGLEGIRLGYGGGFYDRTVAALKPRP
                     FTVGLCYSNGFLPMLKARLDDLPLDALLTDDGVVWQR"
     gene            26677..27051
                     /locus_tag="PKV56_01135"
     CDS             26677..27051
                     /locus_tag="PKV56_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOX66437.1"
                     /translation="MQANDFVEHCLELLQPLGQPRAKRMFGGYGLYLDDLFIAIIADE
                     QLFLKADAATRAAFDAAGCERFTYLARGKLMQMDYWMAPPEAMESPAQMQPWARLALE
                     AALRARTAKRPTARRLRTTKRP"
     gene            complement(27101..27682)
                     /locus_tag="PKV56_01140"
     CDS             complement(27101..27682)
                     /locus_tag="PKV56_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOX66438.1"
                     /translation="MSESSVDAPAAARQALRTRLLAARSELATRTAWPAMQADLAGHL
                     MSVLRQLVPERLGIYSAVRSEFDAAAACLSDPQLCDTPLALPFSHRSPNLMDYRHWDR
                     QPLTLRDECRILAPDGPAVVPDVVLAPCVGYTASGWRLGYGGGYFDRWMAAHPQATVV
                     GVAWSVGEIDEADWVPQAHDRALACVVTERGVV"
     gene            27716..29869
                     /locus_tag="PKV56_01145"
     CDS             27716..29869
                     /locus_tag="PKV56_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOX66439.1"
                     /translation="MSNSRGSAVKCRVPASSNSVYEPGIDGSRSHAVRHGRRLFGALV
                     TMLGIAGAMSWLPGAQAQTPASASARSAPDPVLEARDAYRKRDRARLAALRDATASTQ
                     NPLAMWVAYWDVNVRLSTLQQPDLDAFYSRWSGTYVEDRLRNDWLLELGRRRDWVNFS
                     TDFPRFRMSDDREVTCYALLTEHLAGHDVRDAARNAWFAQRDADDGCALLAGTLLTAK
                     VLRPGDAWRKARVSMDLNRPRAVAQAVTLLQPQADSAVQVLLDAPARYLSDMARANGR
                     VSAELTTLALIKLAAADPDAAAVALRERWERALPDDLAAWAWAATAKQSAMRLSSGAP
                     EQFQRAERLAADSAKASEFADNEWPDDMLGWKVRAALRANQGTGRWQQVVQGINAMTA
                     TEQSEPVWVYWKARALQSLASDSQDGEGLRNQAHEMLGTIAGQLHFYGKLASERLGLP
                     VSLPERPLAITPAERQAAADHPGLNRALQLIAIGLRSEGVREWNYSLLGMTDRELLAA
                     AQRACDRELWDRCINTSDRTRTEIDMQQRFPTPFREQLLAHTREIGLDAAYVYGLIRQ
                     ESRFVMGARSAVGASGLMQVMPSTARWTAKRIGLDYRNDQISDRDTNLKLGTGYLKLV
                     LDDFSGSQPLAAAAYNAGPSRSRRWREGPPLEPAVWAENIPFSETRDYVKKVLSNAAY
                     YAALLGGDAVSLSSRLGKAIGPREAGGPGIDRDLP"
     gene            29917..30873
                     /locus_tag="PKV56_01150"
     CDS             29917..30873
                     /locus_tag="PKV56_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOX66440.1"
                     /translation="MSNVLILGGTGFVGRSVCEKLVERSGGAGGCIRVPSRRPQRAQH
                     IRTLPTIELLPADVHSEAELRRLVDGCDAVINLVAILHGSEADFQRVHVDLPRKLAAA
                     CQASGLKRIVHVSALGAASDAPSMYLRSKAAGEAVLRAAGLELTVLRPSVIFGEHDRF
                     LNLFARLQAVAPVMPLAGAAASFQPVWVEDVASAIAACLDDPSSIGQTVECGGPTVYS
                     LEQLVRFAGRCAGHARPVIPLPDAVARLQAWVMGWLPGEPLMSADNLDSMRAPNVLHG
                     DMPGLEALGIRPASIEAVMTALLAGQAGVARLEPLRARAHRS"
     gene            30898..32094
                     /locus_tag="PKV56_01155"
     CDS             30898..32094
                     /locus_tag="PKV56_01155"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HOX66441.1"
                     /translation="MKAWKVGGAVRDMLLGRPVSDIDWVVTGATPEEMLSAGYTPVGR
                     DFPVFLHPTTKQEYALARTERKTAPGYHGFVFHTDAGVTLEEDLRRRDLTINAMAQDE
                     AGEITDPCGGQRDLEARLLRHVSPAFAEDPVRILRVARFAARFGDFDVASETRALMRE
                     MVQAGEVDALIAERVWQELSRGLMEAHPSRMLDVLQGCGALSRLMPEIDADWQAKGGH
                     AGLLRRAIDLSALRAAPLAVRFAVLTHRLDRPDRGRGATGAARSALQSLCERWRVPSD
                     CREVAEGVAREREAVRRSISMRAEDLIQLLDRLDAWRRRERFELMLLACDCIERAEAT
                     QELPRGQPSHARLRQALRRAAAVDAGGVATHAASQGLRGPAIAAAVHAARVHAADADL
                     NDEPND"
     gene            complement(32115..32915)
                     /locus_tag="PKV56_01160"
     CDS             complement(32115..32915)
                     /locus_tag="PKV56_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain N-acyl amino acid synthase"
                     /protein_id="HOX66442.1"
                     /translation="MLSNSTIGLSPEVHRALHSMLQSPSQSEDLHRCEISQRLFNIRS
                     ADSSGQRSSASILINRMYASRGYPSKPLPDDPQPSRVTLVASEHESTVGTITVGFDSP
                     DGLHVDALFAEQANSLRDAGHAICEFTTLAMDNAVRSRRVLASLFHSAYIYAHRVMDA
                     DNLLIEVNPRHVSYYRRMLGFEVIGPQRFNPRVNAPAVLLCLDFSYAREQIARFGGKP
                     ECGKNERSLYPDFFSPTEEAGIMTRLKPAHFNQQAFSRRVHFPALLQA"
     gene            33210..33524
                     /locus_tag="PKV56_01165"
     CDS             33210..33524
                     /locus_tag="PKV56_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HOX66443.1"
                     /translation="MIKVSVFYANTPGSHFDHGYYCKTHMPMVARLMGSHLKSYSVDK
                     GIAGGAPDSPATYIAAGHLLCESVEAFQAGFGPHAAEILGDIPNYTDLSPIIQISDVL
                     IG"
     gene            complement(33608..33802)
                     /locus_tag="PKV56_01170"
     CDS             complement(33608..33802)
                     /locus_tag="PKV56_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOX66444.1"
                     /translation="MIRWLIAIFLILMIFGGLHRWLERIGLGKLPGDFRFRLFGREFF
                     LPIGSSVLLSMIALALGKLF"
     gene            complement(33869..35044)
                     /locus_tag="PKV56_01175"
     CDS             complement(33869..35044)
                     /locus_tag="PKV56_01175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX66445.1"
                     /translation="MPNEQPSSVSAPPDSPSAAIIPLIREVLVRQGGWLPFDRYMALA
                     LYAPGLGYYARGDRQFGTLPSSGSDFVTAPELSPLFGQALAQQVRQALDASGTDELWE
                     FGAGSGVLARQLLDSLGARVGRYTIVDLSGALRDRQREVLHLHLDRVRWVDALPETMQ
                     GVIVGNEVLDAMPVQLLHFDGQRWLERGVAVGDAREATDDRPSGSSPWIWADRETALR
                     PPVDEAGMVFAPGTVTEIHPQAEAFIATLGDRLERGAAFFIDYGFPASEYYHPQRSGG
                     TLMCHRAHRADADPLADVAAKDITAHVNFTGIAIAGQAAGLDVLGYTSQARFLMNCGL
                     LDLLEGADIRTIANAQKLLTEHEMGELFKVIGFARRCDPAFTTSPIGFAHGDRTHTL"
     gene            35064..35903
                     /locus_tag="PKV56_01180"
     CDS             35064..35903
                     /locus_tag="PKV56_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX66446.1"
                     /translation="MSDRSPPVSRESHPGTRPVALVTGSARRLGREIALDLAAHGFDI
                     AVHYRGEAREALETVSELLSLGAGAHAFHADLSIESACRELLPQVVAHFGRVDAVINN
                     AAMFEYDTPATFSHAAMDLHWKANTAPAVLLAQALHDHHRDHLAAIDPPACVVNLIDQ
                     KLWNPNPDYFSYTLSKAALEAATVMLAQALAPRVRVCGVAPGVTMVSGTMTHAEFEQA
                     HQLTPLKRSSTPADIARTVRFLIDSPAITGTTLLVDGGQHLQGQPRDVMFLAQPTAPA
                     ESP"
     gene            35900..36301
                     /locus_tag="PKV56_01185"
     CDS             35900..36301
                     /locus_tag="PKV56_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOX66447.1"
                     /translation="MTHPLLHPTLADCRRLFLRNHCVSVAIGAHAFEKQAPQRVLFNV
                     ELYVPLSLSTPANDALGEVVDYDFIRNVIAERVARGHIELQETLCDDLLAAMLAHPHV
                     KAALVSTEKPDVYPDCESVGCQVFGLKAEVQ"
     gene            36333..37277
                     /gene="ttcA"
                     /locus_tag="PKV56_01190"
     CDS             36333..37277
                     /gene="ttcA"
                     /locus_tag="PKV56_01190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOX66448.1"
                     /translation="MSAVLTDSPPEIHTPADAKAANEINKLSKRLHRLTGQAIVDFNM
                     IEEGDRVMVCVSGGKDSYSLLDILLNLQKRAPIRFELIAVNLDQKQPGFPEHVLPEHL
                     SRVGVPFHIENQDTYSIVKRVIPEGKTTCGLCSRLRRGILYRVAGELGATKIALGHHR
                     DDIVQTLFMNMFFGSKMKGMPPKLVSDDGRNVVIRPLAYVAETDLERWAEHRQFPIIP
                     CSLCGSQDNLQRVQIKQMLRDWDKRFPGRVENVFSAMQHIVPSHMADRNLFPFAQLQA
                     SGIADVNGDRAFDDDEDCGSVSATGTDAVVTLQRRAES"
     gene            37292..37918
                     /locus_tag="PKV56_01195"
     CDS             37292..37918
                     /locus_tag="PKV56_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOX66449.1"
                     /translation="MRRFCGIAIAWIAVSALAGCASNTLSSEVSTFGGWPADRQPDTY
                     AFERLPSQQIAPEAQQRLEDAARGALESAGFRPADDAGTADVTVQLAARADAMVRSAL
                     DDPLWNSGGLYRSRLEYATFGGFSGGFGFGHGFAIGFDEGMHRYAREVTVLIRDRKSG
                     TALYETRATSDGLLPLTPALLRAMFQAALRPFPTREPQAIRVTTPLTR"
     gene            complement(37925..38875)
                     /locus_tag="PKV56_01200"
     CDS             complement(37925..38875)
                     /locus_tag="PKV56_01200"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HOX66450.1"
                     /translation="MSCSPASNAPGVSDAIIGGVTHVPRSRRWAALRLVITLCALAWL
                     TGCSAVRLAYNQAPDITYWWLDGYVDFSEPQSLRAKEALVDWFSWHRRTELPQYATLL
                     SRARAEVSGPATAEQACRWFDELNVRFDAAFERALPGLADVMRHLSPAQVAHLEHKYA
                     ENNETLAEDYLQDRPEARVKVQFNRALDRIEMIYGRMSGAQRERIAALALQTPFDPQR
                     WLAERRRRQQDTLQTLTRLSGDQATVEQAMVALRVLVQRSRVSPNEEYRDYQQRLVKF
                     NCAFAAQVHNMSTPEQRARAADQLKDWEDDARWLAARAAP"
     gene            38874..40262
                     /gene="glmU"
                     /locus_tag="PKV56_01205"
     CDS             38874..40262
                     /gene="glmU"
                     /locus_tag="PKV56_01205"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOX66451.1"
                     /translation="MALDIVIMAAGKGTRMKSDRPKVLHALAGRSLLEHVLLTAVEVA
                     ADRIVVVTGHGAEQVEAAVRAPQLQFVRQAPQLGTGHAVQQAAPLLHAEGITLILNGD
                     TPLIEAATLRALIEACAGERLALLTVHLADPTGYGRILRHTDGSGATRVQAIVEHKDA
                     SAEQRAITEVYTGAMAVPTAQLRRWLARLDNSNAQGEYYLTDVVAFAVADGVPVVAAQ
                     PRRASEVLGVNSPQQLAELERLCQRDQADALMIAGVRLADPARIDVRGSLQCGTDVAI
                     DVNCVFEGTVSLGNRVQIGANCVIRNAEIAEDAVIHPFTHIDGDTLGVTVGRGALVGP
                     FARLRPGAVLGAAVHIGNFVEVKNSTLAEGSKANHLAYLGDATVGARVNFGAGSITAN
                     YDGANKHRTEIADDVQIGSNCVLVAPLTVGAGATIGAGSTITQDAPAGQLTLARSRQQ
                     STAFWKRPVKRG"
     gene            complement(40249..41163)
                     /locus_tag="PKV56_01210"
     CDS             complement(40249..41163)
                     /locus_tag="PKV56_01210"
                     /inference="COORDINATES: protein motif:HMM:NF020481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HOX66452.1"
                     /translation="MTDPAAQTARELRWLLLSPPLLDSAPGAHAAAVQQFSAAERQTI
                     SGWLDVIERDPAALFDFVHPMNRSGSDGSSPTDQPRAPSPRLGRRAERLMEFFLRHGP
                     THRLVAANIPLRRVSPKGDCTTVGEIDFLLHDRSGRPWHWELAVKFFLCTATGLQASA
                     ADFIGPDRAETFDTKLRKMFERQLSHLPPPPWDNEHWTPAACTRGRLFYRHDRPVPRT
                     PGLHPAHLQGLWIEHDRLGELLGSDQPVWHVVPRHDWMLPRVPLPAGGRDLGVLAALI
                     EADRPGLATMVVRGDAPGEPPVFVLSRA"
     gene            complement(41156..41974)
                     /locus_tag="PKV56_01215"
     CDS             complement(41156..41974)
                     /locus_tag="PKV56_01215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOX66453.1"
                     /translation="MTWTERQQAMLAEMGIRVWDAPVVDAAIEPEAAASGVPPSTPST
                     VVDAPTRGGTERASEGPSQPLVNASLMDWPALREAVASCTACSLCGSRRQTVFGVGHP
                     QAHWMIVGEAPGEEEDRRGEPFVGRSGQLLDSMLHAIGIGRQAGETPDPAKQVFIANT
                     LKCRPPGNRNPLPEELARCEGFLIRQIELVQPRIILAMGRFAVQSLLRSSAPVGQLRG
                     RVHLYQGVPLVVTYHPAYLLRNLPEKARAWEDLCLALDTVEAGARAPVHGAVGD"
     gene            complement(41971..42459)
                     /gene="rimI"
                     /locus_tag="PKV56_01220"
     CDS             complement(41971..42459)
                     /gene="rimI"
                     /locus_tag="PKV56_01220"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOX66454.1"
                     /translation="MNAVLCRWNRLHPMTLVHLDEVMAIEAAAYPFPWTRGNFIDSLA
                     SGYPARVLFDGDRRDHVIGYFVAMEGVDELHLLNLTVVPPEQGRGHGRAMLDALAAMC
                     RDLRSPQLWLEVRVSNTRAREIYRRYGFREVGLRRGYYPAAEGQREDACVMSLDLSGG
                     QL"
     gene            complement(42456..43205)
                     /gene="tsaB"
                     /locus_tag="PKV56_01225"
     CDS             complement(42456..43205)
                     /gene="tsaB"
                     /locus_tag="PKV56_01225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOX66455.1"
                     /translation="MTASLTSCLAFDTATEHLSIALRVGDAIWSHEGPGGALASATLI
                     PGIMDLLAQAGIGLPDLDAIAFGRGPGAFTGLRTACSVAQGLAFGVDKPVLPIDTLLA
                     VAEDARIGGACDDLWVVMDARMDEIYAAHAVFESGRWRYAVVPFLGSPQDLNERWRTE
                     PPLALAGSALSAFGERLDGGAAQRWPAALPRARAMLPLAAALWSQGGAVDAAQALPLY
                     LRDKVAQTMAERAALKASKDALVTATGGVAT"
     gene            43290..44771
                     /gene="dacB"
                     /locus_tag="PKV56_01230"
     CDS             43290..44771
                     /gene="dacB"
                     /locus_tag="PKV56_01230"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOX66456.1"
                     /translation="MLLRALTFACVSAVLLAFGCHVASAQAPSPGLTRALPAEVAAAL
                     DRANVPREALSVVVQDLGRNAAPRLSWQAQAAVNPASLMKLVTTYAALDLLGPAWSWT
                     TPVWLQGALPGAGSGVFEGDVVIKGSGDPKMVIERAWLLLRRLQQLGVRDIHGDIVLD
                     RSAFAVPEQDPAEFDGEPLRPYNVRADALLLNYKSVSFTFTPDTAHGVALISTEPALA
                     GVRADSRVPLAAGVAATQCADWRAALQADFSDPQRLRFKGIYSPACGSKQWPAAYADP
                     AIYNERLLSGLWAEMGGHLSGSVRDGTAPATPPTFELTSPPLAEVIRDINKFSNNVMA
                     QQLFLTLALAQRGLGTPDGAREVVRDWLASRYGDIGRAAVIDNGSGLSRDARVSAQLL
                     ARLLQDAAASPVMPELMASLPVTGVDGTLRRAKALPGRAHLKTGSLRDVTAVAGYVLA
                     PSGRRYVMVGVVNHPNAGAARPALEALIQWTANDGYAEGEALK"
     gene            44782..45075
                     /locus_tag="PKV56_01235"
     CDS             44782..45075
                     /locus_tag="PKV56_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOX66457.1"
                     /translation="MTTPDWVDLLGYAAASLTTASFVPQAWLSFRTGDVSGISLGMYA
                     SFTVGVALWLAYGVVLGAWPIVVANAITLVLAVSILLMKLRGGRTGDRTAPPR"
     gene            complement(45088..46131)
                     /locus_tag="PKV56_01240"
     CDS             complement(45088..46131)
                     /locus_tag="PKV56_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66458.1"
                     /translation="MNFRLDRWSAAALRSCVLAVTAASSLLLASCGGGNGNENQSVKF
                     SAQRLFVYGDESSLLLPPSTPTAIDARKYSIIGFTANTTPANSVRDCLVNPIWVQYVA
                     FKYGLVFAECNPNGAAVTAQMRATYGAKVADVVAALDAFFAAGGSLGKRDLVTVMVGT
                     HDILELYGSVSTGAVTEAAALAEAKRRGELLADQIDRLTNKSNTAGRAIYVPVPDLSE
                     SPFAIGAEAGRTERVRLLKVVSESFNNALRAKVTNNGRSIGLLNAFQRFRNIVRAVDD
                     GDTPFDFVNATQAACTTALPDCTTLTLQPANGTTEAANEFTWVWADDLRLGAGAHDVL
                     GRDAIDLLDTLPF"
     gene            complement(46172..47173)
                     /locus_tag="PKV56_01245"
     CDS             complement(46172..47173)
                     /locus_tag="PKV56_01245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOX66459.1"
                     /translation="MLLDGHNPAAIAAAADALVAGELVAFPTETVYGLGARADDDRAV
                     ARIYAAKGRPVGHPLIVHVVDSQAARFFSDGWPDLAQRLVGAFWPGPLTVIVPRAEGV
                     ASACAGGQPTIGLRCPAHPVALALLRAAAERGVPGVAAPSANRFGRLSPTQAMHVVAE
                     LGPELRVIDGGACEVGIESTIIDCSRDRAVLLRPGTLTRSVIEAVLGSPLEDRDAAAP
                     RAPGTLESHYAPRARVRLMSHDMLRSSLTLLASVPADPASGAPLKLAVYSRLLPPAGA
                     VAFHQAMPARADQAAHELFTVLRRFDDLGAQLIWVEEPPATAEWDGVRDRLLRASAA"
     gene            complement(47200..48375)
                     /locus_tag="PKV56_01250"
     CDS             complement(47200..48375)
                     /locus_tag="PKV56_01250"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOX66460.1"
                     /translation="MTPFIPPGATLGVMGGGQLGRMFVHAAQQMGYFVVVLDADPASP
                     AGMVAQYHIKAGYLDEQGLAQLMQRSAAITTEFENVPAEALMTLGAQRLVAPGAEAVA
                     VCQDRAAEKQHFARCDVPCAPHAVIDSAAGLDSVPDHLLPGILKTTRLGYDGKGQVRV
                     ATRADLSGAWHALGGVSTVLEKLLPLAFEVSVIVARSAAGQMVHFPLQQNLHRDGILA
                     VTQVPAPDLTHAVQQRAIDAAKRVAESLGYVGVLCVEFFVLQDGSVVANEMAPRPHNS
                     GHYTIDACDLSQFDLQVRTMTGAPLAEPRLHSPAVMLNLLGDLWFASGADAQDDRVRE
                     PDWARVLALPGVHLHLYGKATPRRARKMGHLTLTAASVAEVRSVALQAAAILGLAAF"
     gene            complement(48372..48866)
                     /gene="purE"
                     /locus_tag="PKV56_01255"
     CDS             complement(48372..48866)
                     /gene="purE"
                     /locus_tag="PKV56_01255"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOX66461.1"
                     /translation="MSAPAVVGVVMGSSSDWETLRSATEILTEFGVAFEARVVSAHRM
                     PDDMFAYAESAAARGLKAIIAGAGGAAHLPGMLAAKTTLPVLGVPVASRHLQGVDSLH
                     SIVQMPKGIPVATFAIGTAGAANAALFAVAMFAVGDEVLRARLVAYRARQTEAARAMT
                     AQLT"
     gene            48964..49908
                     /locus_tag="PKV56_01260"
     CDS             48964..49908
                     /locus_tag="PKV56_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX66462.1"
                     /translation="MIDITLQNFEADLIHASVDQPILLDVWAPWCGPCKSLGPVLEKL
                     EVAYAGRFKLAKLNADTEPDIAGQLSQMFGVRSIPLCVMFKGGQPVDGFVGALAEAEI
                     RQFLDKHVPTPEELEAEEDVEAAEAMLAEGETDGAIEKLQEAVALNPGNDNARFDCLR
                     ALLMAGRIDDARATFDPVAGKMLLDARLVACGLWLDACEKAVASRSPEVLAAAIAANR
                     RDFDARFELAQLHFAAQRFTDAMDELLEIVMRDKAWNEELARKTYVAVLTMMSKPPAT
                     KSESSAPKGTLELAGKVNTTPADPLVDQYRRKLSMALF"
     gene            complement(49956..51068)
                     /locus_tag="PKV56_01265"
     CDS             complement(49956..51068)
                     /locus_tag="PKV56_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66463.1"
                     /translation="MKAPASPMLARLDAALAASVHPIDVACARAERAGFLARQGFFGQ
                     ANEDLAILRSQFAQRPVALVTVWICIVEAWIEQNSGRLSASRDKMLRAQALSAAARLR
                     PLQALSAAWLAHISFVQDDMDDMAKYLTLAFELAAPDHHAAHARASLVAASAYHFANR
                     LDLAQPWYIRAREHALAESDDAALGVITFTIAGHRSNHALRATIFGSVDTDEARRAEA
                     WVAASFNFDQWVGTIYQDAYVQMLRAAVYSAQGEHAKALQVYEAHLADADRQGLANMR
                     ATHLADQAWCRWHTGDALGARADAEASVALLDPGMNLEDLAVACGRLEQVFQALEDVD
                     AALRYRQLARDHWSRHQEMQTSVIELLDRALKPTPH"
     gene            complement(51065..51973)
                     /locus_tag="PKV56_01270"
     CDS             complement(51065..51973)
                     /locus_tag="PKV56_01270"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOX66464.1"
                     /translation="MTQALLESRLHSLPLLARGKVRDNYAVGTDRLLMIASDRLSAFD
                     VVMGEPIPGKGQLLTEMALFWFERLKDIVPNHLTGGAPESVVAADERAEVVGRSMLVK
                     RLKPLPVEAVVRGYLAGSGWKEYQQSRSVCGVALPEGLVNASKLPQPIFTPATKAEMG
                     DHDENISFERMVEIIGAPLAAQVRDVAIELYRSAAEFALTKGIIIADTKFEFGLDVDG
                     TLTLMDEVLTPDSSRFWPLEGYEAAFSAGLNPPSYDKQFVRDWLEQAVVNGAPWNKQA
                     PAPALPATVVDATAAKYREALQRLTA"
     gene            complement(52011..53075)
                     /gene="fba"
                     /locus_tag="PKV56_01275"
     CDS             complement(52011..53075)
                     /gene="fba"
                     /locus_tag="PKV56_01275"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867836.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOX66465.1"
                     /translation="MPLVSMRQLLDHAAENSYGIPAFNVNNLEQVQAVMSAADEVGAP
                     VILQASAGARKYAGEPFIKHLIQAAVESWPHIPLVMHQDHGQSPDVCQGAMNLGFSSV
                     MMDGSLQADGKTIASYEYNVDVTRKVVEMAHKLGITVEGELGCLGSLETMKGDKEDGH
                     GTDSTMTRDQLLTDPEEAADFVKKTQLDALAIAIGTSHGAYKFTRKPTGDILAIERIK
                     QINKRIPNTHLVMHGSSSVPQDLLAIIRQYGGQMKETYGVPVSEIQEAIKFGVRKINI
                     DTDVRLAMTAATRKYLFENPDKFDPRDFLKPAREAAKQICKQRYLEFNCAGQAAKIKP
                     QTLAAVAARYAKGELAQTVV"
     gene            53327..54346
                     /locus_tag="PKV56_01280"
     CDS             53327..54346
                     /locus_tag="PKV56_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOX66466.1"
                     /translation="MARIDLTALTTWITAAAHDHPRDLPDMLAGLAGVQRQTAVRRLG
                     ELIDLQWLTRTGHGKDVRYAPGLLRQIVRHYALDGLAEDLPWSHDFARYFALPPNVQR
                     LTQHAFCELLNNAIDHSDGTGVTVSLRQTPSHVQLLVSDDGCGVFDKICHTFALEDPQ
                     TAMLELSKGKLTTQPERHTGHGLYFTSRLADVFDLHANDHAFQHRSWEGTGWRPGRPM
                     NRLGTSVYLSIALDTPRTLESVMNAHSLDGTGVRFERTAVPLQLAATPEAGLESRAQA
                     RLVAARLTRFRLAELDFSGIPHIGHSFADELFRVLPQHQPTLNLSPINMSPAVAAMVD
                     SIVTA"
     gene            complement(54353..55783)
                     /gene="pyk"
                     /locus_tag="PKV56_01285"
     CDS             complement(54353..55783)
                     /gene="pyk"
                     /locus_tag="PKV56_01285"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOX66467.1"
                     /translation="MPRATKIVATLGPASSSPEVLERMILAGVDVMRLNFSHGKAQDH
                     IERAAMVREVAKRVGKEVAIMADLQGPKIRVGKFDGGKTMLEAGQSFILDGETTELGT
                     NERVGLDYKELPRDVRPGDTLLLNDGLLKLTVDAVRGAEVHTTVVVGGELSNNKGINK
                     AGGGLTAPALTGKDMEDIKTAMSFQCEYLAVSFPKNATDMEMARQLANVAGEPFKHKA
                     ALIAKIERSEAIPVLESILKASDGIMVARGDLAVEVGNATVPALQKKMIKMARELDRI
                     VITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVETVEMMSTI
                     CIEAERAEEISLDAGFTNKHFGRIDQSIAMAALFTAHHLGCKAILALTESGSTALWMS
                     RHKVHVPIYGLTSQVNSQRKMALYRNVRPLLMASCTDRDQALARAEALLVENGVLKPG
                     DTYAITVGEPMGYPGGTNMLKVCRVA"
     gene            complement(55878..57095)
                     /locus_tag="PKV56_01290"
     CDS             complement(55878..57095)
                     /locus_tag="PKV56_01290"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOX66468.1"
                     /translation="MNAIRFTDLIAANQVAGKRVFIRVDFNVPQDADGRITDDTRIRA
                     SLPCIEAALKAGAAVMVTSHLGRPKEGQWTAEDSLSPIAEHVGKLLGRKVPLIADWVD
                     GVTVDGRALASGDLVLLENCRLNVGESKNSEALSRKMAALCDIYVNDAFGAAHRAHAS
                     THGIAQYAPVACAGPLLAGEADAITKVLAEPKRPLIAIVAGSKVSTKLTILEALSAKV
                     DGLIVGGGIANTFMLAAGLPIGKSLAEADLVDKAKAVISSMKARGAEVPIPVDVVTAK
                     SFAADATATTKAAADVADDDMILDIGPKTAAVLAEKLKSAGTIVWNGPVGVFEFEAFS
                     HGTEAIAHAIAASDAFSIAGGGDTLAAIAKFGVEAGVGYICTGGGAFLEMLEGKVLPA
                     IEILEQRARGAMS"
     gene            complement(57157..57498)
                     /locus_tag="PKV56_01295"
     CDS             complement(57157..57498)
                     /locus_tag="PKV56_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOX66469.1"
                     /translation="MNGSGDAAYVALTVAGLAIVTVITRSFFLLPEREVPIPDWLRRG
                     LKVAPLAALTAVIVPEIVMTQGRLIDSWQDARLPAALAASVWYAWRPGVLGPLLAGLA
                     VFLPLRLLWGW"
     gene            complement(57495..58238)
                     /locus_tag="PKV56_01300"
     CDS             complement(57495..58238)
                     /locus_tag="PKV56_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOX66470.1"
                     /translation="MNPAARLLGDPDFRRGARDMLAMAPGIGAWGMVTGVAMVKGGLS
                     VPLAVLMSFAVYAGSAQLAALPLLAAGAPMWVIWAAAFCVNLRFVIYSAQWRPYFGHL
                     PLRQRMAFGYLAADLNLIAFQRAYPSGPQGPRREPGQIPYYLGGVAVLWSVWQGPSML
                     GIALANLVPTAWGLGFAGTLAMVGLTYGLLSDRSTWVAAVVAGTAAVAAFSLPLKLNI
                     IVAIASAVAAGLLMDQADKASRRLRGNTR"
     gene            complement(58340..58726)
                     /locus_tag="PKV56_01305"
     CDS             complement(58340..58726)
                     /locus_tag="PKV56_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66471.1"
                     /translation="MLRPVVSALISVAALLLTGCEQLGIESPEKIAAAKQAEGSAVGG
                     ACRHAGRAIEDCYALNKRADKAAVFAGWREMNDYMRDNKIEPVPPQLTTEVASAKTTD
                     AESVDAEAPPAKPAKAGKDAKEPKAH"
     gene            complement(58830..59801)
                     /gene="fmt"
                     /locus_tag="PKV56_01310"
     CDS             complement(58830..59801)
                     /gene="fmt"
                     /locus_tag="PKV56_01310"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOX66472.1"
                     /translation="MRLAFAGTPEFARVALAALHQSGFEVALVLTQPDRPSGRGQQPQ
                     PSAVKGWAVERGLTVIQPQGLRLDGRFADDASAAQDSLQAARIDVMVVAAYGLILPEW
                     TLHLPRLGCLNIHASLLPRWRGAAPIHRAIEAGDERTGITVMQMDAGLDTGPMLMAEP
                     LDIFDDDTTGSLHDRLAVLGGRLIVEALEQAARGLLRPTPQPTDGITYAKKVDKAEAP
                     IDWAADACRVAQHIRAFNPAPGAVTQWRGAPLKVWRAQAINASDAGRGVPGTVVAVSA
                     DGIAVACGSGAVRLTEMQRPGGKRMAAAQFLQGHSVQVGDRLEAVAT"
     gene            complement(59844..60353)
                     /gene="def"
                     /locus_tag="PKV56_01315"
     CDS             complement(59844..60353)
                     /gene="def"
                     /locus_tag="PKV56_01315"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOX66473.1"
                     /translation="MSKLTILRFPDPRLHTTARPIEQVDDRIRRLIDDMFETMYAAEG
                     VGLAATQVDVHERLIVIDTSDQHNDQRVLINPELVSASDEQVLGEEGCLSVPTVYDKV
                     LRPARVRVRALGRDGEVVEFDAEGLLAKCVQHEMDHLMGKVFVDYLSPLKRNRIKTKM
                     LKQARDEGR"
     gene            60641..61819
                     /locus_tag="PKV56_01320"
     CDS             60641..61819
                     /locus_tag="PKV56_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOX66474.1"
                     /translation="MTRPIFSALRRRPLAAALGLGLVLTFSWTVPSVAAPNFPVTEQQ
                     RGTAQKVASAGVALSELAPNAPESHTVVRGDTLWGISSLFLKSPWRWPELWGMNIDQI
                     RNPHLIYPGQTLYLDTSDGRARLRLGKAIEGNVVKLKPTVRSSPLSNAAIASIPFSLI
                     EPFLNEAVIFEGNVLDAAPRIVATQEGRVLLTRGDKAYVRGEIGKDTVFRIFREAKPL
                     KDPTTQEILGFEAPYVGTAEFVRSGVNSRVASSSSAETVPDTFIVTSVREEANIGDRL
                     APVTGREYDNYAPHAPQAPLSGQIVSVYGTAVSAGQNQIVTLNRGKSDGVERGHVLAL
                     WHDGSKAIDRTDSRLTELTLPDERHGVLFVFRVFERMSYALILSVQEPVRIGDKFTQP
                     "
     gene            61845..62960
                     /gene="dprA"
                     /locus_tag="PKV56_01325"
     CDS             61845..62960
                     /gene="dprA"
                     /locus_tag="PKV56_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOX66475.1"
                     /translation="MERDELAAWLRLLGTPALGRASARRLLSSFGSPQAAVHATVHAR
                     REVIAEAAASALATPPPDLESSVDATLAWLGDGAVGPGAARREIITLGDPAYPSLLLE
                     TADPPLLLYAQGRLELLAAQGIAIVGSRNPTPQGEQDARAFAKNLSSAGWVVVSGLAL
                     GVDGAAHEGALEGAAGTIAFVGTGLDRVYPRRHLELAHRIALNGLMLSEFPLGTPPLA
                     AHFPLRNRLIAGMTRGTLVIEAALQSGSLITARLASEAGREVFAIPGSIHSPQSRGCH
                     ALIRQGAKLVESAEDVLEELQPGASVLERSAFATSSSANTDPLLDAMGHDLVSLDALV
                     ARTGWPAQELNVRLLELELSGQIARRPGQLFQRVTLA"
     gene            63051..63503
                     /locus_tag="PKV56_01330"
     CDS             63051..63503
                     /locus_tag="PKV56_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HOX66476.1"
                     /translation="MFDVLVYLYENYWRPDACPDHAQLTRKLSSVGFESDEIQEALSW
                     LDGLASAAESYVGEQGEHSLRVYSPAEQEHLGEASIGFISFLESAGVLPPPMREMVLD
                     RASAIPGGPLDLEDLKIIVLMVFWSLGEEPDALILDELFVDEEDRLIH"
     gene            complement(63594..65939)
                     /locus_tag="PKV56_01335"
     CDS             complement(63594..65939)
                     /locus_tag="PKV56_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOX66477.1"
                     /translation="MASTAQTMALATQARRSYVEGLIEGLPGVVHAVDHGARLLLSQI
                     SDPATMMRRRDAVEDIKNASGFWLQGAMSMLRGALHSGLVSASRPNDLPMAGGRQNFS
                     LVDNETIEGEILSSRLALAVMDRASWEFTDLRSRMTMLEGREELDANDILRPHVLARI
                     AITSWRTAGLSHEAWRTLQGVLHEEFAHFNEEAYHEANRLLIQQGVLPEIDLRPLIRR
                     SRNQAFVNTQSGSLAAGPATGLGALASAELTGQPVAALEGAGRAVADDKSVGEETRMM
                     TRAGGLGRGGSDHAEAVLGRLNRLIGRQLPDFARTAHGHLASPGLDAAITSAQQGIVL
                     RLTPEPGARHASAPISTPAMLEELHQRKQALKAATSSPGERATIEVVALLFQSILTEE
                     RIPASVRVWFARLQMPVLRVAVSEPDFFATTDHPARRLIDRMGACVMGFDANSTRLSG
                     GAVEKEIKRVVQVVEAYPDTGRRVFQTVLTEFERFLEHYFKNENEASRKGVSLAQQVE
                     QRETLAIQYTIELRKMLNEMPVQEGVREFLFHVWADVLAMTAVKSGLQSDEIKTMKRT
                     AADLIWSASAKVSREERADVIRRLPPLLKILRDGMQSAGVSLGQQDEHIQRLNNSLAA
                     AFTARTASIPKQRLEQLMERLETLEELLPDAEDVDIDESLMLDLSGHESSELEVVSVG
                     GSMPTPAMVAWARELQVGSWYMLDYRDRNESVQLAWQGMRRQLSLFVSPQGRGILFQQ
                     QRLAAFLQAGLLVPVQDESLTVRATRSALAKLDVDPGRLLN"
     gene            complement(65978..67009)
                     /gene="secF"
                     /locus_tag="PKV56_01340"
     CDS             complement(65978..67009)
                     /gene="secF"
                     /locus_tag="PKV56_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOX66478.1"
                     /translation="MEFFRIRRDIPFMRHALVLNVISALTFVAAVFFIATRGLHFSIE
                     FTGGTVIEVAYAQQADINRVRTTVESLRFGEVQVQNFGTSRDVLIRLPVRADVKQAEV
                     VDRVFAALCKAEGGTVTTQLIKSDKGAESSAPVCGEVGAEKVKLQRSEFVGPSVGSEL
                     ARDGALALAVTIAGIMMYLAVRFEWKFSVAGIIANLHDVIIIVGFFAFFQWEFSLSVL
                     AAVLAVLGYSVNESVVIFDRIRESFRKYRKMTTPQVIDHAITSTISRTIITHGCTLMM
                     VVSMLIFGGPTLHYFAVALTIGILFGIYSSVFVAAAIAMWLGVKRDDLIKPTSKELDP
                     DDPNAGAVV"
BASE COUNT        12081 a        22462 c        21822 g        10904 t
ORIGIN      
        1 tcaggaagaa cagcgcgtag aactggcccg tgtaccagac cacggcctgg ccggctgtca
       61 gacccaccag cgccaggatc acgatcttca ggttcttcca ctggccgaac gattcggtca
      121 gcggcgcctt tgaagtcttg ccttcctcct tcatcttctt gaaggcaggc gattcgttca
      181 tgctcaggcg gatccacacg ctgatcgcca gcaggaagat cgacaccagg aacggcacgc
      241 gccagcccca gtcggcaaag acttcttcgc cgagcatggt gcgggtgccc aggatcacca
      301 tcaggctcag gaacaggccc agcgtggctg tggtctggat ccacgaggtg taggcgccac
      361 gcttgccgtg cggagcatgc tctgccacgt aggtcgccgc accgccgtac tcaccgccga
      421 gtgccagacc ctgcagcatc cgcaggccga tcaggatgac cggcgcagcc acgccgatcg
      481 aggcatagtt gggcagcacg ccgacgatga aggtcgacag gcccatgatc aggatcgtga
      541 ccaggaaggt gtacttgcgg ccaatcatgt cgccgaggcg gccgaacacc agcgcgccga
      601 acggacgcac gatgaaaccg gcggcaaagg ccagcagcgc gaagatgaac gccgagttcg
      661 catcgagacc gctgaagaac tgcttggcga tgatggcggc gagcgagccg tacaggtaga
      721 agtcgtacca ctcgaacacc gtgccgaggc tggaggcgaa gatgaccttc ttctcttccc
      781 cggtcatcgg ccggggcgcg gcagccgcgc ccttcgcgtt gtttcctgtt gtagttgcca
      841 tgtctgttgt ctcctgtggg atatcgcagc accgtccggc gacggaacct gggcagcgat
      901 gatcacgacg gcctctgacc ctgtcctgac gcggcgctga acgcagcctg acaagatgcg
      961 tagaaacccg cgcgttcagt ttctcgatgc gaaattcctg gggtttgcca cacgcacggg
     1021 ggatcgaatt tcagggcccg gcgacagcac ggaccgtcag gtgtcaggca gcggcggccg
     1081 cacgggcagt tcgcgccggc gctgtgtcag ccgcttccag atgacctgct tgtcgcgatc
     1141 gcacagcttt ccccagtcgg tgatctcgtc gcgcgtgcgc aggcagcctg tgcaccagcc
     1201 tgcggtgtca tccaggcaac acacacccac gcacgggctc gggacggacg agccggcgtg
     1261 cggcaaagga ggttggttca tggcagtccc ggagtgaatc agctgcgccg caccggcggg
     1321 cgccatgccg tgatcgtcgt cgccagctgg cgcaggaacc ggccgagcgc ccagatcagt
     1381 ccgaccatcg ccacgaggca cgccagcagc agcgcagcaa aggcgagcgg gtgagtccac
     1441 gacagccaca gcgccgtcgg caccagcgca tcacccagca aggacatgcc gatgttcgaa
     1501 aacggttcgg gtgaggtgtt gacggcggca cgggtcgtcg ccttggcgat gtggctggtc
     1561 gcggccaatg tgccacccat cagcgcagcc gccatagccc acgacgtgtg gtcggccccg
     1621 aacacggccg ccgccaacgc cgcgccggcc ggtatccgca ccagcgtgtg gacgatgtcc
     1681 cacagcgtgt cgagccccgg aatcttgtcg acgaagaact cgatgaacag catgaacccg
     1741 ctcgcgccga gcatcagcgg gctctggagc accttcagcc ccgtcggcag atcgacccag
     1801 cccagcacac cggacaggcc ggtcaggaac acgacggcat agagccgcag accgctggcc
     1861 catccgagtg cggcggcaat cgccagcaac tgggtggtat cgaggggggg catcgtcaga
     1921 acggcccgct cgccagccag cgctcgatct gcgcgcgccg gtcgttgccc cagaacggct
     1981 ccccgtcgat cacgaagaac ggcgcgccga acaccccggc tgagatcgcc atgtcgttga
     2041 cctccttcag tcgtgccttc cagcgcggat cgttccagac ggcctcggcc tgagcggcat
     2101 ccagcccgaa ctccaccgcg atctcgcgca ggcgcgccac atcgctcagg tccgccccct
     2161 gcacgaaaag cgcccgaaag cagtgcttcg cccagccccg ggcatcgagc aagtgggctg
     2221 gatccgggcc gcccgcggac tgatcgcgca gccaccagta aacgcgcgcc gcgttctggg
     2281 tggccacggg aaagcgtgac ggcagcacga acgggacggc tgaaaagcgc gccgagcgct
     2341 cgaagtcgcg cagcgagtac tcgcgcttca gcggatgcgc caccggcgat cgcaactcgg
     2401 ccgcctgaaa ggtcgcgccg agcaggatgg cgttccagtt gacgctgcgg ccgtgctgtg
     2461 ccgccagcgt gtcgatccac tcggaagcca tgtaggcata gggcgacgag agatcgaagt
     2521 agaactcgat cggccgaggt tcgagactca tcgcagcgac ctgctcatgg gttcgccccg
     2581 gaacccgcga aacgttccgc ggcccgtggg cgcaccgggc gtgcactggc aaaccgacgg
     2641 ggcgaaccat tcagtgacca gcagcggcat gccgcgacgc atgaaaaccg accgccgaca
     2701 ccagagcggc tcgcgcgccc accccagacg cgcgccgagg cgccgcgcac gcagcggcac
     2761 catgcgtgag cttcccagac gtgcgcagct cgccgctcgc aagccgaaca gcagatcggc
     2821 cagcggtcgg ctgccgagac cacgcagcgc ccgccaggcc agtcgcgact gcacggcagg
     2881 cagcaccgaa cgcgcataga ccagcggctg cccgttgccc caaagcatca cctcgcgcac
     2941 gtggcagcga gctgcgctca gggccacccc gcctgaccgg cgcaggacgc acagttcgtc
     3001 ggcctgcgcc ggacccgcgc cctgcgacag cacctgcacc tcgaatcgac cgaagacacg
     3061 ggcaagacgc cgactgagcg agccatcgga ctgcaaccag ccgcgcaggg cccgatcgag
     3121 cggcgctgtt ctcacgggtc gaccggccga tcaggaggca gcgaccccgg ccccagcgtg
     3181 tcgctgatgc tcgaatcgct tggcggcttc gtaagggaag acatcgtgca catggcccgc
     3241 caggatgcgc tccttgtgtt cctgccagaa cgcagcgtcg agcaggtcgg cgtggtgctt
     3301 catgaacacc tcgcggacct tggcgttgcc cagcaggaac ggaccgaagg tttcggggaa
     3361 gacgtctttc ggcccgacgc tgtaccagac ctcgccggac atctcttcct cttcgttgcg
     3421 aggagctggc acgcgacgga aattgcagtc ggtgatgtac tcgatctcgt cgtagtcgta
     3481 gaagaccacc ttgccgtggc gcatcacgcc gaagttcttc cagagcatgt cgccagggaa
     3541 gatgttggca gccaccaggt ccttgatcgc gttgccgtac tcgaccaccg cctgcgagat
     3601 ctgctcgggc gaggcttcct ggatgtagat gttcagcggg atcatgcggc gctcgatgta
     3661 cacgtgccgg atcaccaggt cgtcgccgtc ttcctcgagc aggctcggac agaacttctt
     3721 cagctcgtcc accagttcgt cgtcgaagcg tgcgcgcgga aaggccacgt tgctgtactc
     3781 cagcgtgtcg gccatgcggc cgacgcgatc gtgctgcttg accagcaggt acttgctctt
     3841 gatcagctcg cgcgtggttt ccttctgcgg cgggtagtag tccttgatca ccttgaacac
     3901 atacgggaac gacggcaggt cgaacaccag catcaccatg cccttgatgc cgggggcgat
     3961 gcggaactga tcggacgaat gcctcaggtg agcgaggaaa tcccggtaga agaggttctt
     4021 gccctgcttc tgcaggccga gcgcgctgta gatctcgttg cgcggcttgc ggggcatcat
     4081 cgtgcgcagg aactgcacga acgccgacgg cacctccatc tcgaccatga aataggcacg
     4141 ggcgaagctg aagagcagga gcaggtcttc ctcgccgaac agcacggcat cgatcgccag
     4201 cttgccaccc gcgacgtgga ggatcggcag agcaaacggc gtctcccggt agccgttgat
     4261 gaccttgccg accaggtatg cgcccttgtt gcggaagaac agcgagctca gcacctggat
     4321 ctggaaattg gcgcgcggcc gatagtcgcc gagctgcttc tcgacggcgg ccagcacatc
     4381 gtcgatgtcg cgatcgaggt tctcgaaggg gcggcgcagt tcgaagtgat cgacgatccg
     4441 cctccaggtg tcgcgcagcg tggccttggt cgggtagtag gcgaaatagg acggcgtcga
     4501 ggcgggctcg tcgttctcga tgtactcggt cgacacggcg ggccgcagga agatgaaatc
     4561 gttgttgaag tagctgcggt gcaggatctt ggtggtgacc gaattgaaga aggtctccgc
     4621 cagctcgggc tgatggtggt tcgtcagcaa cccgatgtag tgcagcttca cctgctgcca
     4681 gacctcggag ctgagcccgg ctgccttgaa ctcggtctgc aggcgctcgg ctgcctcgtc
     4741 cacgcgcgcg tcgtagtact cgatgcgctc acgctggccg cgctgctggc cgtgccagtc
     4801 ggcttcctcg aagcgacgct tggcctgccg gctggcttcg cggaacaggc ggtaatggcg
     4861 gttgaatccg tcgagcatcg cgcgcgcgat gtcgaacgag cgcacatcgg tcagcgtcgg
     4921 ggggaaattc atctccgcgc ctgacatcaa gtcccctcca cagggctgtc acccgaggct
     4981 gcggaaggtg tgcggcgggc acggagcgca gccagcgccc cctggtaggc atcgcgcacc
     5041 tgttcaggcc gggacacggt catcttcaga tcctccagca atccattgtg cagaccgtag
     5101 aaccagccgt gcacgaccag ctcctgtccg cggttccagg catcctggac catcgtggtg
     5161 tgacagaggt tgcgcgcctg ctcgatcacg ttgagctcgc aaagcgcatc gacgcgctgt
     5221 gccgcgtcga cggtctcgag ccactcctca tgctggttgc gcacatcctg cacgtagcgg
     5281 atccagttgt cggcgatacc cacgcgccgg ttgtggagcg cggcagcgac accaccgcaa
     5341 cccgaatgcc cgacgatgat gatgtggcga acccgcagca tgtcggtggc gaactgcacc
     5401 accgacaggc agttcagatc ggaatgcgcc accacattgg cgacgttgcg gtgcacgaag
     5461 agctcgccgg ggagcagccc gaccagttcg ttggccggca cgcgactgtc cgcgcagcca
     5521 atccacaggt actgcggggc ctgctgcttc agcagattgg tgaagaagcc gggcgtgcgg
     5581 gcctcggtgg cctcggccca catccgattg ttttcgaaca attccttcag atcgccggtc
     5641 atgtgcatgc accccagtag gatggatcga gtctagcgac cgcaggcaac ctcttcgata
     5701 ccttgaaaaa atcaccctcc acccacgccg caccgcaaga accggactac ccctggggca
     5761 acgccgttcc gcaggccggc gacagcatcg aggtcggccc gggcgtgcgc tggctgcgca
     5821 tgcccttgcc gtttgcactc gatcacatca acctgtggct gctgcgcgac gaactcgacg
     5881 tcaccgacgg ctcggtgtcc ggctggacca cggtcgactg cggcatggcc gacgagtgca
     5941 cccgcagcgc ctgggagcag gtcttcgcga cccagctcca cgggttgccg gtgctgcgtg
     6001 tcatcgcgac acacatgcac ccggatcacg tcgggctcgc gcactggctg acgcagcgct
     6061 ggagccgccc ggcctccccc gcgggcgagg ccttcgactg ccggctgtgg atgagcgcga
     6121 ccgaatacgc cgcagcccgg ctcggcagcc ggtccgacaa ctggaccggg ggcgaggctg
     6181 ccgcggcatt catgactgct cacgggctga cagacgctgc ttcgatagag ctgatccgca
     6241 cccgagccgg ctattacgca agcatggtcc cgcaggtgcc cgcagcctac cgtcgcctga
     6301 tggacggcag cgtgctctcg atcggtggcg ttcaggctgc cggacgacac gactggtgct
     6361 gcatcgctgg atatggtcat tcgcccgaac acctggcact gcactgcgca tcgctgggtc
     6421 tgctgatttc aggggacatg ctgctgccca ggatctcgac caacatcagc gtcgggcatg
     6481 acgagcccga gggcaatccg ctggcggcct tcctggcctc gctcgaacgg atgcgcacgc
     6541 tgccggaaga cacgctggtg ctgccctcgc acggcaagcc gttccgtggc ctgcaatccc
     6601 gcatcgacca gctgcaggcc caccacgctg cccggctgtc ggacgtgatg tccgcgtgcg
     6661 ctggttcgcc acgcagcgcg gccgatctgc tgacggtgct gttcaggcga ccactcgacg
     6721 tgcaccagac gacgttcgcc atgggcgagt cgatagcgca cctgaatgcg ctgtggcatg
     6781 acggcaagct ggtgcgccgg ctgcgagacg gcatctatcg gttttcaccg gcctgagggt
     6841 ttgaggcggc ctcgggcagg ccgtgcagcg caaaggttct ggctttcagc cgaagaccgg
     6901 cagcacgtct gtcttcagtg ctcggcgcgt gcgttcgaag tcgggcacga ccgagcggac
     6961 cacatcccag aaccgcggac tgtggttcat ctcgcgcagg tgggcgagct cgtgcgcgac
     7021 gacgtagtcg atcgtcggaa gcccgaaatg gatcagccgc cagctcaggc ggatcgagcc
     7081 gtcggcgctg gcgctgcccc agcgggtctg tgcagacgac aggctgaggc ggcggtagct
     7141 cacaccgagc cgggccgcat aggcgtcgca gcgttcggcg aagacgcgct tcgcctggcg
     7201 ctgcagccag ctctgcaccg catcgcggat ctgctcgggc agcgcaccgg gcggcagtcc
     7261 gatgtgcagc cggcgcggtg gcgcacaccc cgcatccagt gcatcgccag agctggcatc
     7321 gagcacggcg cccgacaagg tgatgccggc ccgtggatcg agcacgacga ccaccgtctg
     7381 gccgagaaac gggatcgtcg cgccatcgcg ccagtcgacg cgggcggact gcagccggcg
     7441 tccgcgctgc tgctgctcgt gcagcttgcc aaggatccag ctggcctttt cgcgcaacgc
     7501 ggattcgatg tcacgtattc cgacccagtt aggcgcgttg acgctcaggc cgtcggtgcc
     7561 gacaatgaag ccgatgctgc gccgacgcac gcgcttgagc gcgtagccca cggtatgccc
     7621 accgagcacg atctcgcggc tgccctgtgg gtggagaaag acggcggcag ccaacggatc
     7681 cgacttggcc gcggtcgcag gagcgtcgaa cgcgacttcg tcggagcgca tcgcggtcga
     7741 cgccgtcggc tcctgcgcca cgaacagcga cagctgcgca ggatcggggc ggtggggcgg
     7801 acgggccatg gcgcggaagt ctacggcccg ggcgcagcgc gaccagcagc gggaagctcg
     7861 acctcggtca gaccgggcgg agccgccgat tcgtgagcgt aagcctcggg atcaaggcgc
     7921 cgcatctcgc cctcgatcca agactcgacc tcgcgcatca gttcgtcggg ccgacgtccc
     7981 gtcgaaggga tcaacggccc gacggaaacg tcgatcacgc cgggtcgcag caggaatgtc
     8041 ttgcgcggcc agcagcgcgc cgaagtcacc gcgatcggca gcaccgcagc acccgtggtg
     8101 accgccaggc gcgtgccgcc ggacttgtac tcgcactggc caccacgctc ggctcgggtg
     8161 ccttcgggaa acatgatgac ccaggcaccg tgtttcatgt gccgagcgcc ctgctcggcc
     8221 acacgccgcc acgcctccga acgccgtgca cggtcgatat ggatcatgtc catgcgcgcc
     8281 atcgcccagc cgaagaacgg gatgtagagc agttcgcgct tgaacacata cgccagcggg
     8341 tgcggcatca ggccggggaa cgcgaaggtc tcccaggtcg actggtgctt gggcaacacg
     8401 atcaccgcgg gcgcccggtt cgcctcggcg aggttgtcga ggccgtgcat gcggtgccgc
     8461 acgccgcaga tcacgcgtgc ggccgagatc gaatgcgtca gccatcccgc gcacagccag
     8521 tagacgcgat cgccgcgcgc gaagatcgac acgatcagca ccaccgtggc ccacgggacc
     8581 acgctcacgg ccatgtagag cagaaagagg aacgacctca ccgcccagaa catgcgctgc
     8641 atcagtgcaa ctccccgaat ccggagtcgg actcgccggt gtcggctcgg tgactgcgct
     8701 cctgccggat cagccagtcg gcgaaagccg acaggtcgtt gtgcacgcag gtgccaggca
     8761 ccgcatcgag cagctctgcg agcgccttgc cttcgaggcc ggcgctcttg ccggtgagca
     8821 ccaggtgcgt cgggcaaccg gctgcggcgc cggcctgcat gtcgcgcagg ttgtcgccga
     8881 ccatcggcgt gttgtccaga gacacgccga accgctcggc gatctgatgc atcatgcccg
     8941 gcaacggctt gcggcaatcg cagccatcgt ctggtgcatg gggacagaag aacaccgcgt
     9001 cgatgcgacc gccctgctgg gccagcgcct gattcagttt cagctggatc gcattcatcg
     9061 atgccatgtc gaacagcccg cgaccgaggc ccgactgatt ggtcgcgagg accgtgtgcc
     9121 agccggcgtg attgaggcgg gaaatggcct ccagcgagcc tggcagcgga acccactcgt
     9181 caggcgactt gacgtaatcg tcgcggtctt cgttgatcgt gccgtcgcga tccaggatga
     9241 cgagcttgag cgaagcagca ggcatgtcag ctcgccagct tggacaggtc ggcgatgcgg
     9301 ttcatcgcgg catgcagttg cgcgagcagg ccaaggcggt tcagccgcag atcgatctct
     9361 tcggcgttca ccatcacgcc gtcgaagaac gcgtccacag gagcacgcaa cgcggccagc
     9421 gcctgcagcg agccggtgtg atcccccgct gcgaactgtg catcggcggc cggcacgacc
     9481 tgcagcatcg ccgcatgcag cgcctgctcg gcaggctctt tcaggagcgc tggattcacg
     9541 tgcgcgtcgg ccccgagtga cttcttcagg atgttggaca cgcgcttgtt cgcggccgcc
     9601 agcgcgggtg cttcgggcaa ctcggcgaag gcacgcaccg ctgccaaacg tcgcggcaca
     9661 tcacccaggc gggtgggagc cagcgccagc acggcatcca cttcctgggc gctgtagccc
     9721 tgatcacgca ggctcacggc caggcgatcg gcaaagaagc ccagcagcgc ctccgtggga
     9781 tcggtcagca ggtcaccaaa ggccggcagc gcagcctgca gcagcgcggg caggtccagc
     9841 ggcagattct tctccgtcag gatgcggatc acgcccagtg catggcggcg cagagcgaac
     9901 ggatccttgt cgccggtggg cagctgtccg atgccgaaca gcccgaccag cgtttccagc
     9961 ttgtcggcga gcgccagcac ggtgccggtg tgattgcgcg gcagcgcgtc gccggcaaaa
    10021 cgcggcttgt aatggtcctc gatggcgatg gccacgccgt cgcgcagtcc ttcgtggcgc
    10081 gcgtagtagc cgcccatcac accctgcagc tcgggaaact cgccgaccat gtcggtcagc
    10141 agatcggtct tggccagcag tgcggactgc tgcaccttgc tgtcgagcac cgcgaattcg
    10201 tcctgagcgt ccacggtgaa cggcagcgtg gccatgctca gctggttgac gatggcgtgg
    10261 ccgatcgcgc gcacgcgctc ggcgcgctcg ccctgggtgc ccagcttgcc gtgatagacc
    10321 accttggaca ggccttcgac tcggctctgc agcgtgcgct tgcggtcctg gtcgaagaag
    10381 aatttcgcat cagccaggcg cggccgcacg acgcgttcat tgccaccgat cacggcgctc
    10441 gggtctgatg gactgatgtt gctcacgatc aggaactggt tggtgagctt gcctgcggcg
    10501 tccagcaacg gaaaatactt ctggttcgcc ttcatcgtca ggatcaggca ctcctgtggc
    10561 acgtcgagga agctctcctc gaaacgcccc agcagaacgt tcggccgctc gaccagtgca
    10621 gtcacctcgt cgagcagcgc gtcgtcatcg atcggggtca gcccctgcag cgatgcggca
    10681 tgctgcaact gccgcgcgat ttcgccatgg cgttgcgcga agccggccat caccgcgcct
    10741 tgctccagca actgctccgc atagcggtcg gcgtgctgga gcaccaccgg atccacggcg
    10801 gcctcgaaac ggtgcccgtg ggtctctcga cccgaattca ggccgagtac cgacaacggc
    10861 accacctcgg cgccatgcag cgcgaccagt ccgtgcgccg gccgcacgaa ctgcacgctg
    10921 gtccatcccg gctgcgcgcc gccttcgagt tgataggtca tcaccttcgg gatcggcagc
    10981 ctggagagcg tctcttcgag cgccttctgc agcccctgcg ccagcgtgat gccaggcacc
    11041 atgctgtcgt agaacagggc ctcggccttg ccatccatct gacgcttgag ctgcggcacc
    11101 accgactcat cggcgcccag gctcgccagc ttcttcagca atgcgggcgt tggtgtgccg
    11161 tcggccgaca gggccacgct gacgggcatc aatttctggc atgcagcctt gtccggggcc
    11221 accgaaagca ccgcgctgac atgcgcggcg agccggcgcg gcgaggcgaa aggcgtcacg
    11281 gctgaatggc cggatgcgag tccctgcgcc cggaggctgt cggccagcac ctgcgcgaac
    11341 gactcgccaa gcttcttcag cgccttcggc gggagctctt cgacgaagag ttcaatcaac
    11401 aaattggctg tcgtggtcat ggaatcaggc agctttcttg tcgcgttgct gggccatggc
    11461 ggcaatcact tcatcggccc aggcacgcgg agccatcggg aatccgagac gcgcgcggct
    11521 gtcgaggtag ctcgcggcga cggcgcgcgc gaggttgcgg atgcgaccga tgtaagcagc
    11581 acgctcggtc acgctgatcg cgccacgcgc gtcgagcagg ttgaacgtgt gcgccgcctt
    11641 caacacctgc tcataggcgg gcagcgcgag ctgctgttcc atcaggtgcc tggcgttgcc
    11701 ttcgtgggcc gtgaaggcgc tgagcaggaa ctcgacgttg ctgtgctcga agttgtaggc
    11761 actctgctcc tgctcgttct gcaggtacac atcgccgtaa ctgatgccgg tgccgtcggc
    11821 ccccatcgtc cagatcaggt cgtagacgtt gtcgacgccc tgcaggtaca tcgccaggcg
    11881 ctccagcccg taggtgatct cgccagtgat cggccggcaa tcgatgccgc cgacctgctg
    11941 gaagtaggtg aactgcgtca cctccatgcc gttgagccag acctcccagc ccagccccca
    12001 ggcgcccagc gtcgggttct cccagtcgtc ctcgacgaag cgcacgtcgt tgaccttcag
    12061 gtcgaatccc agtgcctcga gcgagccgag gtagagctcg agaatgttgc tcggcgcggg
    12121 cttcaacacc acctggtact ggtagtagtg ctgcaggcgg ttcgggttgt tgccatagcg
    12181 gccatccttc ggacgacggc tgggctggac ataggcggcc ttccagggct cggggccgag
    12241 cgcgcgcagg aaggtggcgg tgtggctggt gccggcgccg acctccatgt cgtagggttg
    12301 cagcaaggcg cagccctggg cgtcccagta ctgttgcagt cgcaggatga tttgctggaa
    12361 tgtcagcatg ggcagtgtgt cggggtggtg ggcgcgatgc agccggtctc gacgaggcag
    12421 cgaacgcagc ccttgatttt aggggcgggg gggctgccgc cccctggagc ctcggccgac
    12481 ggccatcgac gcgccgatgg cggccagcac cagcaggatc agcggccaca gtcccagagc
    12541 cgccagccag cgcgcgtagg gcgtcgcacc gacacgtccc tcgaccgtgg cttcgaggat
    12601 gccttcggtc gacgacgtca gtcgcgcggt gacctttccg cgatgatcga ccaccgcggt
    12661 agcgcccgtg ttggtcgacc ggatgaccgg ccgctcgaac tcgatcgcac gcatctgcga
    12721 gaactgcaga tgctgatcct gcaccaacag ccggccaaac caggccaggt tactgacatt
    12781 gacgaagacg gtcgctgcct gcggcccgac gacgctttcg acgatgtcct caccgaacag
    12841 gtcttcatag cagatcaagg ggcgcagtcg ctggccgcca aaggcgaatg ccacctgact
    12901 gcggcctcgc gcctggtcga ccagcgggat gttcatcaga tcgacgaacc agcggaagcc
    12961 aggcgggatg atctcgccga acggcaacag gtggcgcttg ccgtagcggt actcggcacc
    13021 gctcaccgtg ggttcagacg ccgggacggc caccaccgag ttgacgtagc cggattcatc
    13081 atcaccgacg aaaaggccga tgagcgcggc ccgccggctg ccgacggccg cgatgcctgc
    13141 gggagcggca gccggctgaa ccggtgtgtc cagcgtcgca cgcaacgtgt cccaatagcc
    13201 atcgctcaac tgggcccgcg agagcggcag caccgattcc ggcgtcacga ccagctcgcc
    13261 cctggcggcg gacagctgcc ggaccaggcc gcgcagttgc gcctcgagac gctcaggatc
    13321 gaacttgaag tcctgcgaga cgttcggctg cagcaagctg atgcgcagcg tgccggtgga
    13381 ttcagtgaag cgatcaggca gaccgtgcgc catcagcacg acagccacag ctccggcact
    13441 ggtcaccagg cgcatgcgca ccgaagcatc gctcagccac atcgagagca ctgccgccaa
    13501 cgccgccgcc agcgcgcaca gcccgtagac accgatccac ggagcccacg cagcgagcgg
    13561 cccgaccgca tgcgcatagc ccgaggcgat ccatgggaag ccggtgaaga cgagcccgcg
    13621 cgccagttca gcgcccagcc agcacgaagc ccacagcagc aggtcccagg cgatgcgccc
    13681 ggtgcgcagc cgagcccaca gcgccatcgc cgcggcgtaa tacaacccca gcagggctgc
    13741 gagcaccacc acagccagcg ccgccagcac ggggttcaag ccaccgaact gatgcatgct
    13801 gatgtagagc caccagaagc ccgagaccag ccagcccact gcgaacagcc aacccagcca
    13861 cgcagcgcgg cgcggtgcgg cccgcgaagt cgcgtgtgcc agcacgccaa gcgacagcag
    13921 ttgcagccac caccactcga aaggtgagaa cgacaacgtg tgggcgacgc cggcgagcaa
    13981 ggcggccggc gcaccggcca accagcgcgt caccgatgca actcccgacg ccgaaccgcg
    14041 catcaagcgc cgtcggactc gaaggtttct tccggagcgc gtgtgacctt gaaccagcgc
    14101 actgctccac cgcgcgtcag catcacactg aacaacaacc cacccagcga cacggcctcg
    14161 ccacgacgcg gcactcgccc catctcgtgc gctatcagac cgccaatggt gtcgaaggtg
    14221 tcgtgcggca actccgatcc gaaggcctcg ttgacggcgg tgatctccgc atcgcctgcc
    14281 acccggtggc tgccatcggc aagcgtgtag acgctggact cgccgtcctt gtcgtcgaac
    14341 tcgtcttcga tctcgccgac gatctcctcg agcacgtcct cgatcgtgat cagtccggcg
    14401 gtgttgccga actcgtcgat cacgatggcc aggtgattgc ggttcgagcg gaagtcacgc
    14461 agcaactcgt tgagtccctt cgactcgggt acgaacaccg ccggccgcag caacgtgcgc
    14521 aggttcaggt cgggcgagcg ctgcagcttc agcaggtcct tcgccatcag gataccgatg
    14581 acgttgtcgc gctgaccttc gtagaccggg aaccgagaat gcgcggtgcc gatgacgaga
    14641 tgcagcagtt cgtcgtaggg cgcatcgatg tcgaggtgat ccatgtgcgg tgcggccacc
    14701 atcacgtcac cagcggtcat gtccgccatg cggatcacgc cttcgagcat catccgcgac
    14761 tcgggagcta tcagctcgcg ctgttcggcg tcggcgagcg cctcgatcaa ttcatcgcgc
    14821 gagtcgacgc cgggcgacat gaactcgatc agccgctgga acaggttgcg cttgtcggtg
    14881 acggcggttc gtcgcggaga attccggttt cggccggaag aggcgggttg cgccgggtga
    14941 gggtcagaca tcgcgccagg gcggacagtg aaacaccgcc gcagcttacc cgaacggcat
    15001 ttgacaagtg tgctagcttg aaaggctgcg ttccggcccg acatgcctca cacgtttttt
    15061 tcaggacatc agaagcatgg ctctcatccc cgccaccatc ctcaccggct ttctcggctc
    15121 cggcaagacc accctgctca agcgcgtcct gacggaagcg cacggccaga agatcgcggt
    15181 gatcgagaac gagttcggtg acgagaacat cgacaacgag atcctggtgc gcgacaccgg
    15241 cgagcagatc atccagctca acaatggctg cgtttgctgc tcgatccgcg aagacctccg
    15301 caccacgctc agcgacctgg ccgagaagaa gcggaaaggc gagttggtgt tcgatcgggt
    15361 ggtgatcgag accaccggcc tggcagatcc cggtccggtc gcgcagacct tcttcatgga
    15421 cgacgagatc gccgagagct acctgctcga ttcgatactg accctcgtcg acgccaagca
    15481 tggcgaccag cagctcgaca cccggcagga agcgcgacgc cagatcggtt tcgccgatca
    15541 gatattcatc agcaagacgg atctggtcga tgcagcagac gtcgacgcgc tcgcccaccg
    15601 catcaagcac atgaacccac gggcgccgca aagccgcgtg aatttcggcg aagtgcctat
    15661 ttccagcgtg ttcgacctgc gcgggttcaa cctgaacgcc aagctggaca tcgacccgga
    15721 ctttctgaac gacgcagcgc acgaccatga tcatcacgat cacggcgagc actgcgatca
    15781 ccccagccac gcgacccacc atcacgcgca cgacgacgat gtgaaatcct tcgtcttccg
    15841 ctcggacagg gcgttcaacc ccgcgaagct ggaagacttc ctcggctcga tcgtgcaggt
    15901 ctacgggcca aagatgctgc gctacaaggg cgtgctgaac atgaagggtt cgccgaagaa
    15961 ggtcatcttc cagggggtgc accagttgat gggcagcgac ctcggacctg cctgggcacc
    16021 tggcgagaag aagaccagca agatggtgtt catcggcatc gacctgccgc gcgacatttt
    16081 gatgcagggt ttggagcagt gcctcgtgta accgaacccg gagtccggcg gcgtggctac
    16141 aatccgcccc gctttgcaat tcggacagat cgcatcgtcg tggtgcaaaa gtcgcgctct
    16201 atttcgcgct gaggcggtat aaccccgctg cgaggagatt tcagtgaccc gatccccggc
    16261 caagtcagcg cccaccgccg cccgagccga gcccaaatcc aagaaggcag cggacaagct
    16321 gcctgcgaag ccctcgcccg caaaaaaatc tccagcgccc acgccgaaag cgtcggccct
    16381 gccccccccg aagcgggcaa aggcgcctgc caagcgtgcg cccgccacgc ccgcttcgga
    16441 cgtaatttca gccaaggcgg cccgatccgc ctcggccgcc gagaaacccg ctttgaattc
    16501 catgctgaaa acccccttga aggctgccga caaagaggct gcatccgtgc cggcgcccgc
    16561 ccccgttgca gccgtggcgg aagccactgt cgctccagcc aaggcgcctc gggccaagtc
    16621 ggcggccaag tctcgttacg gtgccccgat gccagccgcg ccgctgccgc cgcccacatc
    16681 gcgcttcgcc gaccgctttg cgcccccgcg cccgccaacc cgccagatga acaacctggg
    16741 tgtcgacaca ccgcgtgtgg cgccgaaggc cgatccaaag ctggccaatg cctggaagac
    16801 caaggccggc aaggacctca cggaagccga aatactggcc atgccggaca gcgagtacat
    16861 gaacgagaag caactcgagt tcttccgcgc gaccttgaag aagctgaagg acgacctgct
    16921 gagcaatgca ggcgaaacca ccgagcactt gcgcgaagac acgtccatcg tgcccgaccc
    16981 agcggatcgc gccacgatcg aggaagagca tgcgctcgaa ctgcgcactc gcgatcgcga
    17041 acgcaagctg ctgaagaaga tcgcgcaatc gctgaaccgc gtcgaaagcg gcgagtacgg
    17101 tttctgcgac gagaccggcg agccgatcgg tctcggtcga ctgattgcgc gcccgacggc
    17161 caccttgtct ctcgaagccc agcagcgccg cgaactcaag cagaagatgt tcggcgactg
    17221 accttttcca cgacccgcac gacgcaccga ccccatggcg gaccctaaag acacgaccag
    17281 tttcttccgc aaggtcgtga agttcgtggc gaacccgtcc accgagtgga atgagctcgg
    17341 gtcgggatcg gcgggttcga tcgagaacga tctcgccaaa tccgagctga aggcgatgat
    17401 cgagcgcaag aggcgcaatg acttcgtgcg caagcgcgag ttcgacatgc tgcgcaaggt
    17461 gcgtcgcgag ggactcacgc ccgatcagct ggccgccctg ggcggctcat cggcgatgag
    17521 cgactcgctc cctcgcttgc cggacagcag cggtcggggc tcgggtatga aggcgaagat
    17581 cgacgagatc gagcaacaga tggtcggcaa cagccgcctc ggcaccgtgc gtcgacctga
    17641 cgagttctac tctgcgccca ccgaggccgt accgctcgat gatgctgcaa gccgccgcgc
    17701 atcgcctcca gcagcggctg caaaggccgg ccccgagccg gtgccgtcgg atcactcgcc
    17761 gctgaccttc agcgtccgcg aagacgcgtc ggttttcgac aatccgttcg cagccgcggc
    17821 cgtcaacgag atcgcccacg atccggaact cgatgaagcg gtgatcgctt tcgcgaatgc
    17881 cgatttcgac ctctgcgagc agtcgctgac gcaattgacg cacgcgaacg ggcccagggc
    17941 acagcacgcc gagacgtggc ttgcactgtt cgacatgcac cgcgcaacgg gtcagctgcc
    18001 caagttcgag agcctggccc tcgagtacgc gcagcagttc ggatggtcgg ccccccagtg
    18061 gttctcgatg cccgagcgcg tcgccgaggc cgtcagcgaa gagcgccagc cgcgcgggcg
    18121 catcgacggc caggtcggct ggaccagtcc cgagtggctc gataccgacg ccgtggcgca
    18181 gttgcgatcg cagacgctgc agatgccgct gccctgggtt ttcgactggg gtgcgctgcg
    18241 tggcatcgat gccgaagccg cagcgcgtct gagcgaggtt ttccgcttgt ggatgccgca
    18301 gcggctggac atgcgctggc tcaatgggaa tcgcctcctg agcgtggttc aggaagccgc
    18361 tcctaccggc gtacgcgacg ccgatccggt ttactggcag gtgcgcctcg acgtgttgcg
    18421 catgacgaac cggcccgacc agttcgacga ggccgcgatc gactattgcg tcacatacga
    18481 gatgtcgccg ccgtcctggg aacgatcgca atgccaggtg cgactcagcg gctcgggcaa
    18541 cagcacgctg tcgcactcga tgtctgcggt gagtgacgtc tcgacaggtt tcgtcgaatc
    18601 gcagatcctc gacgacgcgg tgatggtgca ggtgtcgacg gtggaactgt ccggacaact
    18661 ggtcggcgac atcagcgaat tgctgcgaaa catgaccgaa cagctcggga aggcgccctt
    18721 gatcaacgtg acctgtgccc ggctgattcg cgtcgatttc atcgctgccg gcgacctgct
    18781 gaactgggtg ctggcccgcc gcgcagagaa ccggtcggtg acgttcgtcg aagcgcatcg
    18841 cctggtggca ctgttcttcg gcgccatggg tatcaacgag catgcgacgg tgaaggtccg
    18901 cacggtctga tccgcagcca tccgacctgc gccgcccggg gttgaactcc gcggtgccca
    18961 cccgcatcta ccgccgcatg gacaacagca acagcccccc gagctttcac ggcacgacga
    19021 tcgtgagcgt gcggcgcggt gcgcaggtag cgctcggagg cgatggtcag gtgactctgg
    19081 gtcacatcgt catcaagtca tcggcgcgca aggtgcgcaa gctgtaccgg gatcaggtgc
    19141 tggccgggtt cgccggggcg accgccgacg cattcaccct gttcgagcgc ttcgagtcca
    19201 agctcgagaa gcaccagggt cacctggtgc gagccgccat cgaactgaca cgtgactggc
    19261 gcaccgatcg cgtgctgcgt cgcctcgaag ccatgctggc cgtggccgat tcgaccgcat
    19321 cgctgatcat caccggcaac ggcgatgtgc ttgagcccga acatgggatc gtggccatcg
    19381 gatcgggcgg cgcatacgcc caggctgccg ctcgcgcgct gatcgcgcac accgaactgt
    19441 cgccacacga cgtggtgaag aagtcgctcg aaatcgccgg cgatatctgc atctacacca
    19501 accagtcgca caccatagag actctgggat gagcagcaac atgacgccgc aggagatcgt
    19561 ctccgagctc gaccggcaca tcgtcggaca gcacgacgcc aagcgcgcgg tcgcgatcgc
    19621 gctgcgcaac cgctggcggc gccagcaggt cgatgagccc ctgcgcagcg agatcacgcc
    19681 gaagaacatc ctgatgatcg gccccaccgg cgtcggcaag accgagattg cgcgccgact
    19741 tgcgcggctg gcagacgccc cgttcatcaa ggtcgaggcg acgaagttca ccgaggtcgg
    19801 ctatgtcggc aaggacgtcg attcgatcat ccgcgatctg gtcgagaccg ccatcaagca
    19861 gcaacgcgag accgcgatgc gtcgccagcg aacgcgtgca gaggacgctg ccgaggatcg
    19921 catcctcgac atcctggtgc ctccgccgcg aagcgaagcc gcgtcggcgg ccgcgtcagc
    19981 cgacaacacc gcgcggcaga tcatgcgcaa gcggctgcgc gaaggcgcgc tcgacgacaa
    20041 ggacatcgag atcgatcttg ccgaggccaa gccgaccctc gagatcatgg ggccggccgg
    20101 catggaggac atggccgagc aactcaaggg cctgttcgcc aacgtcggtg gtcagaagcg
    20161 caagacccgc aagctgaaga tcggcgacgc gcgccagctc ctggtcgacg aagaggctgc
    20221 caagctggtc aacgaggacg acatcaagac cgccgctctg ctgcagaccg agcagaacgg
    20281 catcgtcttc atcgacgaga tcgacaaggt ggcctcgcgc aacgagacag gcggcgccga
    20341 tgtgtcgcgc cagggcgtgc agcgcgacct gctgcctctc gtcgagggca cggcagtcac
    20401 caccaagtac ggcatcgtca agacggacca catcctgttc atcgcgtcgg gggcgttcca
    20461 cctgtccaag cccagcgacc tgattccgga gttgcagggc cgcttcccga tccgggtcga
    20521 actggcctcg ctcagcgtcg acgatttcga ggccatcctg acccagacgc acgccagcct
    20581 cgtcaagcag tatcaggctc tgctcgcgac ggaaggcgtg acactggaga tcgccgccga
    20641 cggcatccgg cgccttgcac agatcgcgca cgacgtgaac gaacgaaccg agaacatcgg
    20701 cgcacggcgt ctgtccaccg tgatggagcg gctgctcgac gacgtcagct tcgacgcacc
    20761 caaccgcagc ggccagacga tcacgatcgg cgccgcagaa gtcgatgcaa agctggccga
    20821 actcgcccgc aacgaggacc tgtcgcgcta catcctgtaa aggtggacga ctgactcaga
    20881 acgacttcag tcagccacgc cgaagcccgg gtctcccggg ccgacggtgg cgctccttgg
    20941 gggcaggagc gaagcgactg gggggctgac ttttatcgat tgcagggtca gcagtccttc
    21001 gaagagcagc gtgtcgaccg tctcgaaggg caggtcggtg atcggcgtca gcttcacggc
    21061 cgcgccacct gccatcaaca acatcggggc gtgccccgag agctccagca ggcgacggtg
    21121 catgcgctcg accgcacctg caatcgcgtg cgcgccaccg ctcatcagtg catcgctggt
    21181 gttggtcggg aaatcaacga cttcgcccgt cggggccttc aggccggccg tgcccatctc
    21241 gagggcgcgc agcatcagtc cgaagccggg caggatcagg ccgccgatga agcgcccttc
    21301 tgcgtccagg gcatcgacgg tcacggccgt tcccaccatc accacgagtg ctgcacgcgg
    21361 cgcccccgaa gccaacacgc gctggcgcgc cgcaatcagc gcaacccagc ggtcggaacc
    21421 cagccggtac ggatggtcgt agccgttgct gacgccggcg gcccgtgagg tcgagaccac
    21481 ccagcgtggc tccagatgcc acaggtcttc gatctgctcc gaaacgcgcc gcttgactcc
    21541 ctcgcctgcg accacgcagc cgagcatgct cgatggccgg ggcaacagct tccagtcggt
    21601 ttcggccagt cggtcgacgg tctccaggaa gacggcaccc tgcatcagca gttcggctcc
    21661 cggctcggcc gccgaataca gcgcccactt gagtcgggtg ttgccgacat cgacggcaag
    21721 gaagctcatc gcatcggacc cgccagggcg acgccgaagc aggataccca caggtgatcg
    21781 agttgattca gccgttccat cggcggcgat tgtgacggca aaccagcgtg ggtcggcaca
    21841 ctaaacttgt ccgcacaccg ccgctctcgt cggcccgcgc gccgaactcc aacgccatgc
    21901 ctgcatcaga caacgctctg ggtgaatacc ggttcgacgg cgccggcaag ggcaagccgt
    21961 tcaacctgaa acgcattgac ccgtccgaca agccctgctc cagcggagac aaggccgccg
    22021 accggtccgc agtgaccgat ctcgcgctcg agatcgacgc cttgcagaac ctgctgtacg
    22081 cgaatcgcaa acggcggctg ctggtggtgc tccagggtct cgacaccagc ggcaaggacg
    22141 gcacggtgcg cggcgtattc agcgccacca gtccgctggg cgtgcacgcg gttggctgga
    22201 aggcccccac cgaggacgaa ctggcgcacg attacctgtg gcgcatccac cagcgcatgc
    22261 cggccgccgg cgagatcatc gtgttcaacc gcagccacta cgaggacgtg ctcgtaccgg
    22321 tcgtcaacgg ctggatcacg ccggagcaga ccaagcagcg atacgcccac atcaacgact
    22381 tcgagcgcat gctggtcgag acgggcaccg tggtgctgaa gttcatgctg cacgtctcga
    22441 aggaagaaca gcgtgtgcgc ctgcaggagc gcatcgatga tccaaccaag cactggaagt
    22501 tcagcattgg cgacctcgag gtgcgcaagc gctgggacga ttatcagaag gcctacgcag
    22561 atgcgatctc ggcgaccggc acgacttggg cgccctggac ggtggtgccg gccgactcga
    22621 agacgcatcg caacctgatg gtcgccacgc tggtcacacg cgcgctgcgc gagttggatt
    22681 tgcagccgcc cccggccgac ccggcaatct cgaaactgaa ggtcgagtga gcctcgccga
    22741 cgcgcttgat gcggtggatc gctaccgcga acgggtgcgg gcccaggcgc agctcgcgcg
    22801 cgacatccag catctgcagg aagtcgcgcg catgctcttc gaggacaagc ccgacaagac
    22861 caaggcggtc gaagccgtca acgacatcgc cgccaaacgc ctcgccagga tggatgaagg
    22921 cgcgcggcag ttgctcgact cggaaccgcc gatcaagaac tggaatggat cgcatgaccg
    22981 cggtgagctg atccgctggc tgatgctcgt caatcgaccc ggacagtttc cctacacgca
    23041 gggaatcgca gagcccggtg cgcctaaagg cgatgccccc cacgcagcgt cacgagcgcg
    23101 ggtgacgctc ggtgcgcctc cagacgaatc gacgggatcc gaccacgcgt tgtacctggc
    23161 ggccgcgctg aaggatggct gggcgcttct tgaagcgaca aagcgagatt cggcaacagc
    23221 cgggctggct gagctcgctt cgcagattgc catcgcattc gacgagcaca gttgcgcgcg
    23281 acagtgcgcg ctggctcgtg ccgcgcggcg aatctgggcg gtgagcctgc gagaacactt
    23341 cggggtccgt ggcgacagcc tgaagctgaa gctggtatcg acccgcagct cgcaaggtgt
    23401 caacccgtcc gaactcgaga tcagcgaccg tgccaccgat caggccgagg acacgctgct
    23461 ggctgccctg cagcgtctgg tcaagggttc ggcaaggagt tgacgaaggt tcgggtcgca
    23521 gccggcgcct cgatgcgcag cgcggtctcg ggagtcgcca cgcagggcag gatgcaaccc
    23581 tcgcgctttt cctcggcgct caagccgggc cactcgatca ggtgccgcac cgttccggcg
    23641 agcacccggc acaggcaggc tctgcaggtc ccgttgcgac aggacgtggg cagcacgtag
    23701 ccagctgtgc gggccgactc gaccaacgag acgccgccgc gcgcttcgaa ctgccacccc
    23761 tgcggttcga tgacgaccgc gaaccgatcg acgggctccc cgctcacttc gctggatcgt
    23821 ccggcaggcc gaagaccacc acgctgtcgc cggtcttgag cccgaacagc gaatttccgc
    23881 cggcagcgac cgcgacgaac tgccgaccac cgatcgaata cgtgatgggc ggtgcattga
    23941 caccggcctc gcaggcatgg gaccacaact gcttcccgga tgcgctgtcg atggcagcga
    24001 aaacaccatg gccgatgccg ctgaagatca gccccgaggc cgtggacaac acgcccccga
    24061 tcaacggctc gtcgatcttc acctgccaac gcaagcggcc ggcctgacgc aggtcgagcg
    24121 cgctcagcgt gccgccccgc tcgtcgctgt tctcggtgct ggcgtactga agaacgctgc
    24181 catcgggccg cgtcacttcg tgcgcgatgt agcgcgtggg caggtgcgtc gcgggaacga
    24241 atatcaagcc gagcgatgcg tcgtaggccg aaggcgacca gttcgcaccg cccgcgatgc
    24301 ccggcgcgac cagcacgccg tcaccgggct gtggcggcgt gaacaggttg cgttgcggaa
    24361 cgaacgcatc ggacttgtaa aggaatcgcc catcgcgccg atcgtgcaca tagacccagc
    24421 ccagcttgct cggcgaggcc agtgccggca ggcgttgccc gtcgacgtca agatcgaaca
    24481 gcaccggggg gctggccacg tcgtagcccc agcgatcatg aggaacctgc tgctggtacc
    24541 accgcagctt gccgctgcgg gcgtcgagtg cgaccaggga cgaggtgtag aggttgtcgc
    24601 caggccgcga ggcatcggcc atgttgggcg aagggttgcc ggtaccgaag atcagcaggt
    24661 ctcgctcgac atcgacgacc ggtgtgacgt agatggcccc cccgccgaac ttccaggcat
    24721 cggcgtgggc ggcagcgcgg gccttctcgt cggtgatgtc gcgatgcgtg gtcaagccgt
    24781 cggtggtggc atcgcggaac gagccctccc agcccgggcc ggtgatgtcg aaacgccaga
    24841 gcgcccggcc ggtctgcgca tcgaaagccg ccatcagccc cggctggccg tactgacccg
    24901 aaacgccgac caccgacaga ccctggtccg ggtgcagacc gtagcccaca ccgctgatgc
    24961 cgatgtacac ccggccgtcg tgcaccagcg gggccgcgcc tatgccgaca ccggtcgagc
    25021 cggtggcagc gacccgggac atcgtgtctt cggcgcgcag ctggctgggc gcctcggtgc
    25081 tgcccgcgta ctccgccaca gacacatccc acagcttgga gccggtcttc tgatcgagag
    25141 cgaccagccg tccgtcggcg ctgcccaggt agaccttgcc accggacacg gcgacacccc
    25201 ggctcgccgg tccgcagcaa agctgcttcc agcgcatcac atgcttgtag cgccacaact
    25261 cctggccggt ggccgcgtcg agggccacca cgtgactgaa cggcaaagac acgaacatca
    25321 cccgatccac cacgatcggc gtggcctgga aggtggccga cacgccgctg tggaatgtcc
    25381 acttgggcac cagccgaccc acgttcgccg cgttgatgcg ttcgagcggc gaatgtcttt
    25441 gaccggccag gttctgaccg tgcagcgccc agggttcatc ggccatcgcg ctgcggtccg
    25501 cagccagcaa ggcgcacgat gaagccagcg ccgccaggcc cagcgccact gcacgcatca
    25561 caccgacccg agcgctgcga agatcggaca tggcgcgatt caactcgcga tcgaggcgac
    25621 ccagatcaag gcacccgcgc atccgacggc cagcaccgca actgcgaaca ccaggatctg
    25681 gacgcgcctg cgaactgcgt cgattgcttc gaccagctgg gcgttctgcg cggccagcgc
    25741 gtcgagcagt tccgcagcag cctgttgccg ctcggttgcc tgcgcgagcg aagactccag
    25801 tgcgccgatg cggcgatggg cggcggcgag cgggtcgccc gcttcggcct cggaggaagg
    25861 cgccgttgcg ccatcggctg cagtccgagc cgcgtcctgg gtccgcttga agaatccacg
    25921 cgcactcttg gccagccccg gggcggcatc gatcacggtg ccccagggga tgaccttcag
    25981 cgcctgaagc caaacggcag ccatcgagtg ctccgtaatc cgctcagcgc tgccagacga
    26041 cgccgtcgtc ggtcagcagc gcgtcgagcg gtagatcgtc cagccgtgcc ttcagcatcg
    26101 gcagaaagcc gttggagtag cacaagccga ccgtgaaggg ccgcggcttg agcgcggcca
    26161 cggtgcggtc atagaaacca ccgccgtagc ccagacggat gccttcgagc ccgtaaccca
    26221 cacagggcag cagcaccatc tgcggatcga aaacctcggt gcctttgggc ttcgggatgt
    26281 cataagcgtc cggctccatc gggcagcccg ggtaccagac gtggaacttc agcgcgccgg
    26341 cctcacggtc caccactggc aaaccgatcc gtctctcatc cgagccctcg gcccagcggt
    26401 acagcgcagg cagcgggtcg aactcgccct tgatcggcca gtagccgcca atggcggttt
    26461 ccttgcggtg caccagccag acgcgcaaca cactctgcaa ctgcaccgcg cgctccagtc
    26521 gatcgggcag tgcctgccgc gctgcgatca acttgacacg cagggcggca cgctcgtcgg
    26581 ggacgtcgga gacttcggca gggtcaggtg aactcatgga cggattgtgc aagactggca
    26641 accaggtcag gacaccatcg gaacaccgat cccactgtgc aagcgaacga tttcgtcgaa
    26701 cactgcctgg aactgctgca gcccctcggc cagccgcgag ccaagcgcat gttcggcggg
    26761 tacgggctct acctagacga cctgttcatc gcgatcatcg ccgacgagca actgttcctg
    26821 aaggccgacg ctgcaactcg cgcggccttc gatgcggcgg gctgcgaacg attcacgtac
    26881 ctcgctcggg gcaagctgat gcagatggac tactggatgg cgccgccaga ggcgatggag
    26941 tcccccgccc agatgcagcc atgggccaga ctggcgctgg aggctgcgct gcgcgcccgg
    27001 accgcgaaga ggccgactgc ccgacgcttg cgcacgacaa agcggccctg aagcccgcga
    27061 tcgccagcac acagccaagg cccgggccaa tggcgccggc tcagaccacg ccgcgttcgg
    27121 tcaccacgca ggccagcgca cggtcatggg cttgcggcac ccaatcggcc tcgtcgatct
    27181 cgccgaccga ccacgccacg ccgaccacgg tggcctgcgg atgggcagcc atccagcggt
    27241 cgaagtaccc gccgccatag ccgagccgcc agccggatgc ggtgtagccg acgcacgggg
    27301 cgagaaccac gtcgggaacg acagcggggc cgtcaggggc gaggatgcgg cattcgtcgc
    27361 gcagggtcag cggctggcga tcccagtggc ggtagtccat gagattcggg ctgcgatgcg
    27421 agaacggcaa cgccagcggc gtgtcgcaca actgcggatc ggacaggcaa gccgccgccg
    27481 catcgaattc agatcgaact gcgctgtaaa tgccgagtct ttccgggacg agttgcctca
    27541 acaccgacat aagatgaccc gcaaggtcgg cctgcatggc aggccacgcg gttcgggtgg
    27601 ccagctcgga gcgcgccgca agcaatcgcg tgcgcagggc ctgacgggct gcggccggcg
    27661 cgtcgacgga ggattcggac atggatggat gggcgggatg tggagtaacg cgaacgtgag
    27721 caacagcagg ggcagcgcgg tgaaatgccg cgttccggct tcgtcgaatt cagtatatga
    27781 gccgggcatc gacggctcca gaagccacgc cgtgcgccat ggccgccggc tgttcggtgc
    27841 gcttgtgacg atgctcggca tcgccggggc aatgtcatgg ctgcccggcg cccaggccca
    27901 gacaccggca agcgcatccg cgcgatcggc gcccgatccg gtgctcgaag ctcgcgacgc
    27961 ctaccgcaag cgcgaccgcg cgcggctggc tgccctgcgc gatgcgacgg catcgacgca
    28021 gaacccgctg gcaatgtggg tggcctactg ggacgtcaac gtccgactga gcaccctgca
    28081 gcagcccgat ctggacgcct tctacagccg ctggagcggc acctatgtcg aagaccggtt
    28141 gcgcaacgac tggctgctcg aactcggacg ccggcgcgac tgggtgaact tcagcaccga
    28201 ctttccgcga ttccgcatga gtgacgatcg cgaagtgacc tgctacgcgc tgctcaccga
    28261 gcacctggcg ggtcatgatg tgcgagatgc cgcacgcaac gcctggtttg cccagcgcga
    28321 cgctgacgac ggatgtgcgc tgctcgccgg cacgctgctg acggccaagg tcttgcgacc
    28381 gggagacgca tggcgcaagg cgcgtgtctc catggatctc aaccggccgc gcgcagtggc
    28441 gcaggctgtc accctccttc agcctcaggc agacagtgcc gtccaggtgc tcctggatgc
    28501 accggcacgc tatctgtcgg acatggcgag ggccaacgga cgcgtcagtg ccgaattgac
    28561 gacactggcg ctgatcaagc tggctgccgc cgatcctgat gccgctgcag tggccctgcg
    28621 cgaacgctgg gagcgggcac tgccggacga cctggccgcg tgggcgtggg ccgccacggc
    28681 aaagcagtcg gcgatgcgcc tgtcgagcgg cgcgcctgaa cagttccagc gcgccgaacg
    28741 gctggccgcc gactccgcca aggcatcgga gttcgcagac aacgaatggc ccgacgacat
    28801 gctcgggtgg aaagtacgcg ctgcgttgcg agccaaccag ggcaccggcc gttggcagca
    28861 ggtggtccag ggcatcaatg caatgaccgc cacggaacag agcgagccgg tctgggtcta
    28921 ctggaaagcg cgcgccctgc aatcgctggc ctcggattcc caggatggcg aaggactgcg
    28981 caaccaggcg cacgagatgc tcgggacaat tgccggccag ctgcacttct acggcaagct
    29041 ggccagcgag cgcctcgggc tgccggtgtc tctgccggaa cgtccgttgg cgatcacccc
    29101 cgcagagcgc caggcggccg ctgatcaccc gggcctgaac cgggcgcttc agctgatcgc
    29161 gatcggcttg cgcagcgaag gcgtgcggga gtggaactac agcctgctgg gcatgaccga
    29221 ccgtgaattg ctggccgctg cgcagcgcgc ctgcgaccgc gagttgtggg accggtgcat
    29281 caacacgagc gatcgcacac gcaccgagat cgacatgcag cagcgcttcc cgacgccgtt
    29341 ccgggagcag ctcctcgcgc acacgaggga gatcggtctc gacgcggcct atgtgtacgg
    29401 gttgatccgc caggaatcac gattcgtgat gggcgctcgt tccgcggtcg gcgcatcggg
    29461 gctgatgcag gtcatgccgt ccaccgcacg ctggacggcc aagcggatcg gactcgacta
    29521 ccgcaatgac cagatctcag accgggacac gaacctgaag ctcggaaccg gctacctgaa
    29581 gctggtgctc gacgacttct ccgggtcgca gccactggcc gccgccgcgt acaacgccgg
    29641 cccgagccga tcccgccgct ggcgggaagg cccgccgctg gagcccgcgg tgtgggcgga
    29701 gaacatcccg ttcagcgaga cgcgcgacta cgtcaagaaa gtgctctcca acgccgccta
    29761 ttacgctgcc ttgctgggcg gcgacgcggt gtcgctgtcc agccgactgg gcaaggcgat
    29821 cggcccacgc gaggctggcg gacccggcat cgacagggat ctgccctgac cgcgcagaat
    29881 cggtcgcttc tagtcaacgc tggagggctg cagcccgtgt cgaacgtcct gatcttgggt
    29941 ggtaccggtt tcgtcgggcg cagcgtctgc gaaaagctcg tcgagcgctc cggcggcgcc
    30001 ggcggctgca tcagggtccc cagcaggcga ccccagcgtg cgcaacacat ccgcacgctg
    30061 cccaccatcg aactgctccc tgccgacgtg cacagcgaag cggagttgcg ccggctggtc
    30121 gacggctgcg atgcggtcat caatctggta gccatcctgc acggcagcga agccgacttt
    30181 caacgcgtcc acgtcgacct gccgcgcaag ctggccgccg cctgtcaggc cagcgggctg
    30241 aagcggatcg tgcatgtcag cgccctcggt gcggcgagcg atgcaccctc gatgtacctg
    30301 cgttccaagg ccgctggcga agccgtgctg agggccgccg gcctcgagct gacggtgctg
    30361 cggccgtcgg tgatcttcgg cgagcacgat cggttcctga acctgttcgc ccgcctgcag
    30421 gcggtggcac cggtgatgcc gctggccggc gcggcagcga gcttccagcc cgtatgggtc
    30481 gaggacgtgg cctcggccat cgcggcctgt ctcgacgacc cctcttcgat cggtcagacg
    30541 gtcgaatgcg gcggacccac ggtctacagc ctggaacaac tggtgcgttt cgccggtcgc
    30601 tgcgccggac acgcgcgacc ggtgatcccg ctgcccgatg ccgtggcccg actgcaggcc
    30661 tgggtgatgg gctggctccc gggcgaaccg ttgatgtcgg cagacaacct cgattcgatg
    30721 cgcgcaccga atgtgctcca tggtgacatg ccaggcctgg aagcgctcgg tatccggccc
    30781 gcttcgatcg aggcagtgat gacagccctg ctggccggac aggccggcgt ggctcgcctc
    30841 gaaccgctgc gtgcccgcgc gcatcgaagc tgatcgcgat cgggcggcct tgcacccatg
    30901 aaggcatgga aggtcggcgg cgccgtacgg gacatgttgc tgggccgtcc ggtcagtgac
    30961 atcgactggg tcgtcactgg cgccactccc gaggagatgc tgtcagccgg ctatacgccg
    31021 gtgggacgcg atttcccggt gttcctgcac ccgaccacga agcaggaata cgcgctggcc
    31081 cgcaccgaac gcaagacggc accgggctac cacggcttcg tgttccacac cgatgcgggc
    31141 gtgacactcg aggaagacct gcgacgccgc gacctgacga tcaacgcgat ggcccaggac
    31201 gaagccggtg agatcaccga tccctgcggc ggtcagcgcg atctggaagc caggctgctg
    31261 cgccacgtct ctcctgcatt cgccgaagac ccggtgcgca tcctgcgggt cgcgcgcttc
    31321 gcggcgcgct ttggcgattt cgacgtggca tccgagaccc gggccctgat gcgcgaaatg
    31381 gtgcaagccg gtgaggtcga cgcgctgatt gccgaacggg tctggcagga gctgtcgcgc
    31441 ggtttgatgg aagcccaccc gtcgcggatg ctcgacgtac tgcaggggtg cggtgcactt
    31501 tcgcgactga tgccggaaat cgacgccgat tggcaagcca aaggcggtca cgccggtctg
    31561 ctgcgtcgcg ccatcgacct cagcgcgctg cgcgccgcac cgctcgcggt gcgctttgcc
    31621 gtgctgacac accggctgga tcgaccggat cgtgggcgtg gggcgaccgg cgccgctcgg
    31681 agcgcattgc agtcactgtg cgagcgctgg cgagttccgt cggattgccg cgaggtcgcc
    31741 gaaggcgtcg cgcgggagcg cgaagcggtg cgacgcagca tctccatgcg cgccgaagac
    31801 ctgattcagt tgctggatcg cctggatgcc tggcgacgcc gtgagcggtt cgagctgatg
    31861 ctgctggcat gcgattgcat cgagcgcgcc gaggctacgc aagaactacc gagaggccaa
    31921 ccgtcccacg cccgcctccg ccaggcactg aggcgggctg cagcggtcga cgcgggaggc
    31981 gtggcgacgc acgccgcctc acaggggttg cgcggacctg ctatcgctgc ggccgtccac
    32041 gcggcgcgcg tgcatgcggc ggatgccgac ttgaacgacg aaccgaacga ttgaggattt
    32101 gcaaagtcac cacatcaggc ctgcagcagg gcgggaaagt gcacgcgccg actgaaagcc
    32161 tgttgattga aatgcgctgg cttgagccga gtcatgatcc ccgcctcttc ggtcggggaa
    32221 aagaaatccg gatagagcga ccgctcgttc ttcccgcact ccggcttccc gccgaacctt
    32281 gcgatctgct cacgcgcata ggaaaaatcc aggcacagca atacggccgg cgcgttgaca
    32341 cgtgggttga agcgctgagg cccaatcact tcgaagccca gcatccgacg gtagtaggac
    32401 acgtggcgcg gattgacttc gatcagcaga ttatcggcat ccataacgcg gtgggcatag
    32461 atgtaagccg aatggaataa agatgccaag acccggcgag accgcaccgc gttgtccatc
    32521 gccaaagtgg tgaattcgca aattgcatgc cctgcatctc gcaaagaatt ggcctgctcc
    32581 gcgaacaagg cgtcgacgtg caatccgtcc ggggaatcga atcccacggt gatggtgcct
    32641 accgtggatt cgtgttcgct tgcaaccaat gtgactctgg aaggctgtgg gtcatcaggg
    32701 agcggcttgc tgggatatcc gcgcgaggcg tacatccggt tgatcagaat gctggccgaa
    32761 ctgcgctggc ccgaggagtc tgcgctgcga atattgaaaa gtctctggct gatttcacag
    32821 cgatgcaggt cctcactttg cgaagggctt tggagcatcg agtgcagggc gcgatgcact
    32881 tcgggagaca gaccgatcgt cgaattgctt agcacgagaa agccctcaat gaggttgagg
    32941 taatcggcgg accgatgaac ggaatcgccg gggtgggcta ttcaaaccag gcatcattta
    33001 aacggaattt tcacacgctt ggtgggaatt caagcaagca ttggtaacag acggcggcga
    33061 agggtcggga ccagtcaccg gattcggatg gagtcccaag caagtcctga cagcacccga
    33121 ttgcccgaca ggctgctcgt gaggcacgat gcgcttggat gccttttgct ggcatttcaa
    33181 ccacaacgac tcatcgacag gacaagaaca tgatcaaggt cagcgtcttc tacgccaaca
    33241 cccctggcag ccatttcgac cacggctact actgcaagac gcacatgccc atggtggcca
    33301 ggctgatggg tagccacctc aagtcgtact cggtcgacaa gggcatcgcc ggcggagcgc
    33361 cggacagccc ggcgacgtac atcgcggcgg gacatctgct gtgcgaatcc gtcgaggcgt
    33421 ttcaagccgg tttcggccct cacgccgccg aaatcctcgg cgacatcccg aactacaccg
    33481 acctgagtcc gatcatccag atcagtgacg tactgatcgg ctgaactggc tcgagggacc
    33541 gttgcaccat aaccgtccct cggccggcgg catcgacatc aaggatgccg cccgctcggg
    33601 attcgggtca gaaaagcttg cccagagcca gcgcgatcat gctgagcagc acgctgctgc
    33661 cgatcgggag gaagaactcc ctgccgaaaa gccggaacct gaaatccccg ggcaatttac
    33721 ccaggccgat ccgttcaagc cagcggtgca gtccaccgaa gatcatcagg atcaggaaga
    33781 tcgcgatcag ccagcgaatc acgttggctc cccgcactcg cgcacgatcc ccagcccttg
    33841 gcagccaagg gcatggccat ggcgcccttc acagcgtgtg tgtgcgatcg ccatgagcga
    33901 atccgatcgg tgaagtggtg aaggcgggat cgcaccgcct ggcgaagccg atgaccttga
    33961 acagttcgcc catctcgtgc tcggtcagca gtttctgagc gttggctatg gtgcggatgt
    34021 cggcgccctc gagcaggtcg agcaggccgc agttcatcag gaagcgggcc tggctggtgt
    34081 agcccagcac gtcgagaccg gccgcctgcc cggcgatggc gatccccgtg aagttgacgt
    34141 gcgcggtgat gtccttggct gcaacatcgg ccagcggatc ggcgtcagcg cgatgggcgc
    34201 gatggcacat gagcgttccg ccgctgcgct gcgggtggta gtactcggat gcgggaaaac
    34261 catagtcgat gaagaatgcc gcgccgcgtt ccaggcggtc gcccagggtg gcgatgaaag
    34321 cctccgcctg cgggtggatc tcggtgaccg tgccaggcgc gaagaccatg ccggcttcgt
    34381 cgaccggcgg ccgcaacgcg gtttcgcggt cggcccagat ccacggcgat gaacctgaag
    34441 gcctgtcatc ggtcgcctcg cgggcatcac ccaccgccac cccgcgctcg agccagcgct
    34501 gcccatcgaa gtgcagcaac tgcacgggca tggcatcgag cacctcgttg ccgacgatca
    34561 cgccttgcat cgtctcgggg agcgcatcga cccagcgcac gcggtcgaga tgcaggtgca
    34621 gcacctcgcg ctgcctgtca cgcaacgctc ccgacaagtc gacgatggtg tatcgcccga
    34681 cccgcgcacc gagcgagtcg agcaactggc gcgcgagcac gcccgagccg gctccgaact
    34741 cccagagttc atcggtgccg ctggcgtcga gcgcctgccg gacctgctgc gccagcgcct
    34801 ggccgaacaa cggagacagt tcaggtgcgg tcacgaaatc gcttcccgac gagggcagcg
    34861 tgccgaactg gcgatctccg cgggcgtagt agccgaggcc gggtgcataa agcgcaagcg
    34921 ccatgtagcg atcgaatggc aaccagccgc cctggcgaac cagaacctct cggatcagcg
    34981 ggatgatcgc tgcgctgggc gaatcggggg gcgccgatac acttgacggc tgttcgttgg
    35041 gcatcgcccg gattgtagaa accatgtccg atcgttcgcc cccggtatcg cgcgaatcgc
    35101 accccgggac ccgcccggtc gcactggtca ccggttcggc gcgtcggctg ggccgcgaga
    35161 tcgcactcga tcttgcagcg cacggtttcg atatcgcggt gcactatcgt ggggaagccc
    35221 gggaggcgct cgaaacggtg tccgaattgc tgtcactggg tgccggggcg cacgcgtttc
    35281 acgccgacct gtccatcgaa tccgcctgtc gcgagttgct gccgcaggtc gtcgcgcatt
    35341 tcggtcgggt cgacgcggtc atcaacaacg cagcgatgtt cgagtacgac acgccagcca
    35401 ccttcagcca cgcggcgatg gacctgcact ggaaggcgaa caccgcacca gcggtgctgc
    35461 tggcacaggc gctgcacgat caccaccggg accatctcgc cgcgattgac ccgcccgcct
    35521 gcgttgtcaa cctgatcgat cagaagctct ggaatccgaa tcccgactat ttctcgtaca
    35581 ccttgtcgaa ggcggcactc gaggccgcga cggtgatgct ggcacaggcg ctggcaccgc
    35641 gggtgcgggt atgcggtgtc gcccccggcg tgacgatggt gtcgggcacg atgactcacg
    35701 ccgagttcga gcaggcccac cagctgacgc ccctgaagcg ctcctcgact ccggcggaca
    35761 tcgctcggac ggtgcgtttc ctgatcgaca gcccggcgat caccggcacg acgctgctgg
    35821 tcgatggagg ccagcatctg caaggccagc cgcgcgacgt gatgttcctc gcgcaaccga
    35881 cagcccccgc tgaatcgcca tgacgcatcc gctgctgcac ccgaccctcg ccgattgccg
    35941 ccggctgttc ctgcgcaacc actgcgtctc cgtcgccatc ggcgcgcatg cgttcgagaa
    36001 gcaggctccg cagcgggtgc tgttcaacgt cgagctgtat gtgccgctgt cgttgtcgac
    36061 gcctgcaaac gacgcgcttg gcgaagtggt cgactacgat ttcatccgca acgtcatcgc
    36121 cgagcgggtg gcgcgtggcc acatcgagtt gcaggaaacc ttgtgcgacg acctgctggc
    36181 ggccatgctg gcgcatcctc acgtcaaggc cgccctggtt tcgaccgaga agcccgacgt
    36241 gtatccagac tgcgagtcgg tcggctgcca ggtgttcggc ctgaaagccg aagtgcagtg
    36301 acgaacgcag ccgtctgttc gaaggaacct tcatgagtgc cgtactcacc gactcccctc
    36361 cagagatcca cacgcccgcc gatgcgaagg ccgcgaacga gatcaacaag ctcagcaagc
    36421 ggctgcaccg cttgacaggt caggccatcg tcgacttcaa catgatcgag gaaggcgacc
    36481 gcgtgatggt ctgcgtgtcg ggcggcaagg acagctactc gctgctggac atcctgctca
    36541 acttgcagaa gcgcgcaccg atccgtttcg agctcattgc cgtcaacctc gaccagaagc
    36601 agcccggatt ccccgagcat gtgcttcccg aacacctgtc cagggtgggc gtgcccttcc
    36661 acatcgagaa ccaggacacc tattcgatcg tgaagcgcgt gatccccgag ggaaagacga
    36721 cttgcgggct gtgctcgcgg ctgcggcgcg gcatcctgta tcgggtggcc ggcgaactgg
    36781 gcgcgacgaa gatcgcgctc ggccaccacc gcgacgacat cgtgcagacg ctgttcatga
    36841 acatgttctt cggcagcaag atgaagggca tgccacccaa gctggtcagt gacgacggaa
    36901 ggaacgtggt gatccgcccg ctggcgtacg tggccgaaac cgacctggaa cgctgggctg
    36961 agcatcggca gtttccgatc attccgtgct cgctgtgcgg cagccaggac aacctgcagc
    37021 gggtgcagat caagcagatg ctgcgcgact gggacaagcg cttcccgggc cgtgtcgaga
    37081 atgtcttcag cgcgatgcaa cacatcgtgc cgtcgcacat ggcggatcgg aacctgttcc
    37141 ctttcgcaca gctccaagcg agtggtattg ccgatgtgaa tggcgaccgt gcgttcgatg
    37201 acgatgagga ttgcggctcc gtttccgcca caggcaccga tgcggtggtc accctgcaga
    37261 ggcgcgccga atcctgagga ggagttccgt gatgcgccgt ttctgcggga tcgcgatcgc
    37321 ctggatcgcc gtttcagccc tggccggatg cgccagcaac acgctgagca gcgaggtgtc
    37381 gaccttcggt ggctggcccg ccgatcgcca gcccgacacc tacgcgttcg agcggctgcc
    37441 atcccagcag atcgcacctg aggcccagca acggctcgag gacgccgcac gtggcgcact
    37501 cgaaagcgcg ggattcaggc cggccgacga tgcaggcacc gccgatgtca cggtccagct
    37561 ggcagcgcgc gccgacgcga tggtccggtc cgctctggac gatcccctgt ggaacagcgg
    37621 cggtctttac cggtcgcggc tggagtacgc aacattcggc ggcttcagcg gtggcttcgg
    37681 attcggccac ggtttcgcca tcggcttcga cgaaggcatg catcgttacg cacgcgaagt
    37741 gaccgtcctg atccgtgacc gcaagtcggg tacggccctt tacgaaacac gggccaccag
    37801 cgacgggctg ctgcccctga cgcctgcgct gctgcgtgcc atgttccagg ctgcgctgcg
    37861 tccgttcccg acccgcgaac cccaggcgat cagggtcacg acaccgctga cgcgctgagc
    37921 cgcgtcaggg cgccgctctg gctgccagcc accgggcatc gtcctcccaa tccttgagct
    37981 ggtcggccgc cctggcacgc tgttcgggcg tggacatgtt gtgcacctgc gcggcgaatg
    38041 cgcagttgaa cttcaccagt cgctgctggt aatcgcggta ctcctcgttc ggcgacacgc
    38101 gcgagcgctg caccagcacc cgcaacgcaa ccatcgcctg ttcgaccgtg gcctgatcgc
    38161 cgctcagccg ggtgagcgtc tgcagcgtgt cctgctgcct ccgcctgcgc tcggccagcc
    38221 aacgctgagg gtcgaagggc gtctgcagcg ccagagcggc aatgcgctcg cgctgggcac
    38281 cgctcatgcg tccgtagatc atctcgatgc ggtcgagcgc gcggttgaac tgcaccttga
    38341 cacgtgcttc gggacggtcc tggaggtagt cctcggcgag cgtctcgttg ttttcggcgt
    38401 acttgtgctc cagatgcgcg acctgcgccg gcgacaggtg ccgcatcacg tcggccagcc
    38461 cgggcaacgc tcgctcgaag gcggcgtcga agcgcacatt gagctcatcg aaccagcggc
    38521 aggcctgctc ggcggtcgcg ggacccgaga cctcggcacg cgcacgcgac agcaaggtcg
    38581 cgtactgcgg cagttcggtg cgccgatgcc aggaaaacca gtcgaccagg gcctctttcg
    38641 ctcgcaacga ctgcggctcg ctgaagtcga catacccgtc gagccaccag taggtgatgt
    38701 cgggcgcctg gttgtaggcc aggcgcaccg cactgcagcc agtcagccac gccagggcgc
    38761 acagcgtgat caccagcctc agcgcggccc atcgccgaga acgagggacg tgcgtaaccc
    38821 cgccgataat tgcgtccgac acaccaggtg cattcgaggc aggggaacag gacatggcgt
    38881 tggacatcgt gatcatggcg gcaggcaagg gcacgcggat gaaatcggac cgccccaagg
    38941 tgctgcacgc cctggcgggc cgcagcttgc tggagcacgt gcttctcact gcagtcgaag
    39001 tggccgcgga ccgcatcgtc gtggtcaccg ggcacggcgc agagcaggtg gaagctgccg
    39061 tgagagcgcc acaactgcaa ttcgttcgcc aggccccgca actcggcacc ggccacgcgg
    39121 tgcagcaggc agcccccctg ctgcacgctg aaggcatcac gctcattctc aacggagaca
    39181 cgccactgat cgaggcggcg accctgcgcg ccctgatcga agcctgcgca ggcgagcggc
    39241 tggccctgct gacggtacat ctggccgatc ccacgggcta cggccgcatc ctgagacaca
    39301 ccgatggttc cggagcgacg cgggtccagg caatcgtcga gcacaaggac gccagcgccg
    39361 agcagcgtgc gatcaccgag gtctacaccg gcgcgatggc ggtgccgacc gcgcagctgc
    39421 ggcgctggct ggcacggctc gacaacagca atgcgcaagg ggagtactac ctgacggacg
    39481 tcgttgcgtt cgccgtggcc gacggcgtgc cggtggtcgc cgcgcagccg cgacgcgcgt
    39541 ccgaggtgct gggcgtgaac agcccccagc agctcgccga actcgaacgc ctgtgccagc
    39601 gcgatcaggc cgatgcactg atgattgccg gggtgcggct cgctgacccg gcacggatcg
    39661 acgtccgagg cagcctgcaa tgcggcaccg acgtggccat cgacgtcaac tgcgtcttcg
    39721 agggcacggt cagcctgggc aacagggtgc agatcggcgc gaactgcgtg atccgcaacg
    39781 cggagattgc cgaagatgcg gtcatccatc cgttcacgca catcgacggc gacacactgg
    39841 gtgtcaccgt cggacgcggc gcactggtcg gtccgtttgc ccgcctgcgc cccggcgcag
    39901 tgctgggtgc ggcggtacac atcggcaact tcgtcgaggt gaagaactcg acgctggccg
    39961 aaggctcgaa ggccaatcac ctggcttatc tgggcgacgc gacggtgggc gcacgcgtca
    40021 atttcggcgc cggcagcatc accgccaatt acgacggggc caacaagcac cgcaccgaga
    40081 tcgcggacga cgtgcagatc ggcagcaact gcgtgctggt ggccccgttg acggtcggtg
    40141 ccggagccac gatcggcgca ggctcgacga tcacgcagga tgcgccagct ggacaactga
    40201 cgctggcgag aagcaggcag cagtcgaccg cattctggaa acggccggtc aagcgcggct
    40261 gagcacgaag accggcggct cgccaggcgc atcgcctcgc accaccatcg tcgcgaggcc
    40321 gggccggtcg gcttcgatca gcgccgccag gacacccagg tcgcgcccgc cggcgggcag
    40381 cgggacgcga ggaagcatcc agtcgtggcg aggcacgacg tgccacaccg gctgatccga
    40441 gccgagcagt tcgccgagcc gatcgtgctc gatccagagg ccctggagat gcgcgggatg
    40501 cagtcccggc gtgcggggca ccggcctgtc gtggcggtag aaaaggcggc ctcgcgtgca
    40561 ggcggcaggc gtccagtgct cgttgtccca gggcggcggc ggcaggtggc tcaactgacg
    40621 ctcgaacatc ttgcgcagct tggtgtcgaa ggtctcggcc cgatcggggc cgatgaagtc
    40681 ggcagcgctg gcctgcaggc cggtcgcggt gcacaggaag aacttcaccg caagctccca
    40741 gtgccacggc cggccggaac ggtcgtgcaa caggaaatcg atttcaccga ccgtggtgca
    40801 gtcacccttc ggcgaaacac gccgcagcgg gatgttcgcg gccaccagcc gatgcgtcgg
    40861 accatggcgc aggaagaact ccatcagccg ttcggcacgc cgccccagac gcggcgaagg
    40921 tgcgcgcggc tgatcggttg ggctgctgcc gtcggatcct gaccgattca ttgggtggac
    40981 aaagtcgaac agcgcagcgg gatcgcgctc gatcacatcg agccatccgg agatcgtctg
    41041 acgctcggca gcgctgaact gctgcaccgc ggccgcatgt gcgccgggcg cgctgtcgag
    41101 caacgggggc gaaagcagca gccagcgcaa ctcgcgcgct gtctgagcgg cagggtcagt
    41161 caccgaccgc gccgtgcacc ggcgcgcggg cgccagcctc gacggtgtcg agtgccaggc
    41221 acaggtcctc ccaggcgcgg gctttctccg gcaggttgcg cagcagatag gccgggtggt
    41281 acgtgaccac cagcggcaca ccctggtaaa ggtgcacacg cccgcgcaac tggccgacag
    41341 gcgccgaact gcgcagcagg ctctgcaccg cgaaacgtcc catcgcgagg atgatcctgg
    41401 gctgcaccag ttcgatctga cggatcagga aaccttcaca acgcgcgagt tcctcgggca
    41461 agggattgcg gttgcccggc ggtcggcatt tcagcgtgtt ggcgatgaag acctgcttcg
    41521 ctggatcggg cgtctcgccg gcctggcgac cgatgccgat ggcgtgaagc atgctgtcga
    41581 gcaattgacc tgaccggccg acgaaaggct cgccgcggcg gtcctcctct tcgcccgggg
    41641 cctccccgac gatcatccag tgggcctgcg gatgcccgac gccgaacacg gtctgccgac
    41701 ggctgccgca caggctgcag gcagtgcatg acgcaaccgc ttcgcgcagc gcgggccagt
    41761 ccatcagcga ggcattgacc aacggctgcg acggtccttc gctggcccgc tcggtcccgc
    41821 cccgggtcgg cgcatcgacc acggtcgacg gcgtcgatgg cggcaccccg gaagcggccg
    41881 cctcaggctc gatcgcggca tccaccaccg gcgcatccca gactcggatg cccatctcgg
    41941 cgagcatcgc ctgctgtcgc tcggtccagg tcacagttga ccgccggaca ggtcgaggct
    42001 catcacgcag gcgtcttcgc gctgaccttc ggcggccggg tagtagccgc gccgcagacc
    42061 gacctcacgg aaaccgtagc gccggtagat ctcgcgggcc ctcgtgttgc tgacacgaac
    42121 ctcgagccac agctgcggcg agcgcaggtc gcggcacatg gcggcgagcg catccagcat
    42181 cgcgcgacca tgcccgcgtc cctgttcggg tggcaccacg gtcaggttca gcagatgcag
    42241 ctcgtcgacg ccctccatgg cgacgaaata cccgatgacg tgatcgcgcc ggtcgccatc
    42301 gaagagcacc ctcgccggat agccggacgc cagcgaatcg atgaaatttc cgcgagtcca
    42361 ggggaaggga taggccgcag cctcgatcgc catgacctcg tcgaggtgca ccagcgtcat
    42421 cggatgcaac cggttccagc ggcacagcac ggcgttcatg tcgccacccc accggttgcc
    42481 gtgaccagcg catctttcga ggccttcaac gcggcgcgct cggccatggt ctgggcgacc
    42541 ttgtcacgca gatacagcgg caatgcctgc gccgcatcga cggctccccc ttgcgaccac
    42601 aacgctgcag ccagcggcag catcgcccgg gcgcgcggca gggcggccgg ccagcgctga
    42661 gccgcaccgc catcgagtcg ctcgccgaac gccgacagcg ccgaccctgc gagcgcgagc
    42721 gggggctcgg tgcgccagcg ctcgttcaag tcctgcggac tgccgaggaa cggcaccaca
    42781 gcgtatcgcc agcgtcccga ctcgaacacg gcatgcgcgg catagatctc gtccatgcgc
    42841 gcgtccatca cgacccacag gtcgtcgcac gcccccccga tgcgcgcgtc ctccgcaacg
    42901 gcgagaagcg tgtcgatcgg cagcacaggc ttgtcgacgc cgaaggccag gccctgcgct
    42961 accgagcagg cagtgcgcag gccggtgaac gcccccggcc cgcgaccgaa agcgatggca
    43021 tcgagatcag gcaagccgat gcctgcctgc gccagcaggt ccatgatgcc ggggatcagc
    43081 gtggccgagg ccagcgcccc gccgggacct tcgtgcgacc agatcgcgtc gccgacccga
    43141 agcgcgatgc tcaggtgttc ggtggcggta tcgaaggcca gacaggaggt cagggaggcg
    43201 gtcacgcaac gagtctagct gcggtgcatg tgtgtttcag cggcagggat tcgccttccg
    43261 atcgcggatg cccatgcgat cattcccgga tgctcctgcg tgccttgacg ttcgcctgcg
    43321 tgtctgctgt cctgcttgcc ttcggctgcc atgtcgcaag cgcgcaggcc ccatccccgg
    43381 gcctgacgcg cgcgctgccg gccgaggtcg cggccgcgct ggaccgcgca aacgtgccgc
    43441 gcgaagcgct gtcggtggtg gtgcaggatc tgggccgcaa cgcagcgccg cgcctgtcct
    43501 ggcaggctca ggcggcggtc aatccggctt cgctgatgaa gctggtcacc acctatgcgg
    43561 cgctcgatct gctcggcccg gcctggagct ggaccacgcc ggtgtggttg cagggagcgc
    43621 tgccaggcgc gggcagcggc gtcttcgagg gcgatgtggt catcaagggc agcggcgacc
    43681 cgaagatggt gatcgaacgc gcgtggctgc tgctgcgccg gttgcagcag ctgggcgtgc
    43741 gcgacattca tggcgacatc gtgctggatc gcagcgcctt cgcagtgccc gagcaggatc
    43801 ctgccgaatt cgacggagag ccgctgcgcc cctacaacgt gcgcgccgac gcgctgctgc
    43861 tgaactacaa gtcggtgtcc ttcaccttca cgcccgacac ggcacacggc gtcgccttga
    43921 tctccaccga gccggcactg gccggcgtgc gcgccgactc ccgggtgcca ctggctgccg
    43981 gcgtcgccgc gacccagtgc gcagactggc gtgcggcatt gcaggccgat ttctccgatc
    44041 ctcaacggct gcggttcaag ggcatctact cgcccgcctg cggctcgaaa cagtggccgg
    44101 cagcctatgc cgacccggcc atctacaacg aaaggctgct gtccgggctg tgggccgaga
    44161 tgggggggca cctgagcggc agcgtgcggg acggaacggc acccgccacg ccaccgacct
    44221 tcgagctgac ctcaccgccg ctggccgagg tgatacgcga catcaacaag ttcagcaaca
    44281 acgtgatggc ccagcaactg ttcctgacgc tcgcactggc gcagcgcggg ctcgggacgc
    44341 cagacggggc tcgcgaggtc gtgcgcgact ggctggcgtc gcgctatggc gacatcggtc
    44401 gcgcggcagt gatcgacaac ggctcggggc tttcgcgcga cgcccgcgtc agcgcacagt
    44461 tgctggcccg gctgctgcaa gacgcggcag ccagccccgt gatgccggaa ctgatggcat
    44521 cgctgccggt cactggagtg gacggaacgc tgcgacgcgc caaggcgctg cctggtcgcg
    44581 cgcacctgaa gaccggctcg ctgcgcgacg tgaccgcagt ggccggctac gtgcttgcac
    44641 cgagcggtcg ccgctacgtg atggtgggag tggtcaacca cccgaatgcc ggcgcagcgc
    44701 ggccggcgct cgaggcgctg atccagtgga ccgccaacga cggctacgcc gagggcgagg
    44761 ccctgaagtg aggacgccac gatgaccacg cccgactggg tcgatctgct tggctacgcg
    44821 gcagcatccc tgacgacggc ctccttcgtg cctcaggcct ggctgagctt tcgcaccggg
    44881 gatgtcagcg gcatctcgct cggcatgtat gcgagcttca cggtcggtgt ggccctgtgg
    44941 ctggcctacg gcgtggtgct gggcgcctgg ccgatcgtgg tggccaatgc gatcaccctc
    45001 gtgctggccg tttccattct gctgatgaaa ctgcgcggcg ggcggaccgg cgaccggacc
    45061 gccccgcctc ggtgaggctc ccccggatca gaacggcagg gtgtcgagca gatcgatggc
    45121 gtcgcgtccg agcacgtcgt gcgcccccgc gccgagtcgc aagtcgtctg cccacaccca
    45181 ggtgaattcg ttcgcggctt cggtggtgcc gtttgccggc tgcagggtca gcgtcgtgca
    45241 atcgggcaat gcggtcgtgc aggcagcctg cgtggcgttg acgaagtcga acggggtgtc
    45301 accgtcgtcc accgcccgca cgatattgcg gaagcgctgg aaggcgttga gcaggccgat
    45361 gctgcgtccg ttgttggtga ccttggcgcg gagcgcgttg ttgaacgact cggagacaac
    45421 cttcagcaat cggacacgtt ccgtgcgacc ggcttccgca ccaatcgcga acggcgactc
    45481 gctgaggtcg gggaccggca cgtagatggc acgaccggcg gtgttgctct tgttggtgag
    45541 gcggtcgatc tgatcggcga ggagttcgcc gcgtcgcttc gcctcggcca gggctgccgc
    45601 ttcggtgact gccccggtgg acacgctgcc gtagagctcg aggatgtcgt gcgtgccgac
    45661 catgacggtg accaggtccc gcttgccgag gctgccgccc gcggcaaaga aagcgtctag
    45721 tgcggcgacc acatccgcga ccttcgcgcc gtaggtcgcg cgcatttgcg ccgtgacagc
    45781 agcgccgttc gggttgcatt cagcgaaaac gagcccgtac ttgaaggcca cgtactgcac
    45841 ccagatcgga ttgaccagac agtcacgaac cgaattggcc ggcgtggtgt tggcagtgaa
    45901 cccgatgatg gagtacttgc gcgcgtcgat cgcggtgggg gtcgacggtg gcagcaacag
    45961 gctgctttcg tccccgtaca cgaagagccg ctgcgcgctg aacttcacgg actggttttc
    46021 gttgccgttg ccgccaccgc aggaagcgag cagcagcgag ctcgctgccg tcacggccag
    46081 cacgcagctg cgcagtgcgg cggcgctcca tcgatcgagt ctgaaattca tgcggtctcc
    46141 aacgagaaag gattaggtcg gccggggtgc ctcacgcggc cgaagcccgc agcaagcggt
    46201 ctcgcacccc gtcccattcg gcggtggccg gaggttcttc cacccagatg agctgcgcgc
    46261 ccaggtcgtc gaatcgacgc aacacggtga acagttcgtg ggcggcctga tcggctcggg
    46321 ctggcatggc ctggtgaaat gccaccgcgc cagccggcgg aagcaggcgc gaatataccg
    46381 ccagcttcag aggcgccccg ctcgccgggt cggcaggcac cgaggcgagc agcgtcaggg
    46441 acgagcgcag catgtcgtgg ctcatcaacc ggacccgggc gcgcggggcg tagtgggact
    46501 ccagggtgcc gggagcccgg ggggcggcag catcgcgatc ctcgagcggg ctgccgagca
    46561 ccgcctcgat gaccgatcga gtcagtgtgc cgggccgaag cagcacggcg cgatcgcgcg
    46621 agcaatcgat gatggtcgat tcgatgccga cttcacatgc ccctccgtcg atcacccgca
    46681 actcgggccc cagttcagcg acgacgtgca tggcctgcgt cgggctcagg cgcccgaagc
    46741 gattggcact gggagccgcg acgccgggca ccccacgctc ggcggcggcc cgcaacaggg
    46801 ccagcgccac cgggtgagcc gggcatcgca ggccgatcgt cggctgccca ccggcacagg
    46861 cggacgccac gccctccgca cgcggcacga tcacggtcaa ggggcccggc cagaaagcac
    46921 cgaccagccg ttgcgccaga tctggccagc cgtcactgaa gaagcgagcg gcctggctgt
    46981 cgaccacatg cacgatcagg gggtggccga ccggccggcc tttcgccgca tagatccgcg
    47041 caaccgcccg gtcgtcgtcg gcgcgcgcgc ccagaccgta gaccgtctcg gtcggaaacg
    47101 ccaccagttc accggccacc agcgcgtcgg ctgccgcggc tatcgcggcg ggattgtgtc
    47161 cgtccagcaa catgttgcga tgccgggctt cgggcgcaat cagaacgcgg ccagaccgag
    47221 gatggcagcc gcctgcaacg ccacgctgcg cacttcggcc acggaggcag cggtcagcgt
    47281 caggtggccc atcttgcggg cgcgccgcgg ggtggccttg ccgtaaaggt gcagatgcac
    47341 gccgggaagc gcgagcaccc gcgcccagtc gggttcgcgg acccggtcgt cctgcgcgtc
    47401 agcgccggag gcaaaccaca ggtcgccgag caggttcagc atcacggcgg gcgaatgcag
    47461 ccgaggttct gccagcgggg cgccagtcat cgtgcgcacc tgcaggtcga actgcgacag
    47521 gtcgcacgcg tcgatggtgt agtggccgga gttgtggggg cgcggcgcca tctcgttggc
    47581 aaccacactg ccatcctgca gcacgaagaa ctcgacgcag agcacgccga cgtatccgag
    47641 tgattcggcc acgcgcttgg ccgcatcgat ggcacgctgc tgcacggcat gcgtcaggtc
    47701 gggagccggc acctgagtga cagcgaggat gccgtcgcga tgcaggttct gctgcaaagg
    47761 gaaatgcacc atctgcccgg ccgcgctgcg cgcgacgatc acgctgacct cgaaagccag
    47821 aggaagcagc ttctcgagca ccgtggacac gccgcccaga gcatgccagg cgccagacag
    47881 atcggcccgt gtcgccacgc gaacctgccc cttgccgtcg tagccgaggc gggtggtctt
    47941 caggatgccg ggcaacaggt ggtcgggaac cgaatcgagc cctgccgcac tgtcgatcac
    48001 cgcatgcggc gcacacggca cgtcgcaacg cgcgaagtgc tgcttctcgg ccgcacggtc
    48061 ctggcacacc gcgaccgcct ccgcgcctgg cgcgaccagg cgctgcgctc ccagggtcat
    48121 cagcgcctcg gcgggcacgt tctcgaactc agtggtgatc gcagcgctgc gctgcatcag
    48181 ctgcgccagg ccctgctcgt ccagatagcc ggccttgatg tggtactgag cgaccatgcc
    48241 ggcggggctc gcagggtcgg catcgagcac cacgacgaag taccccatct gctgggcggc
    48301 atgcacgaac atgcgaccga gctgtccacc gcccatgacg cccagggtcg caccgggcgg
    48361 gatgaagggc gtcatgtcag ttgagccgtc atggcacgcg ccgcctcggt ctgccgggca
    48421 cggtaggcaa cgagccgggc gcgcaagacc tcgtcaccga cggcgaacat cgccacagcg
    48481 aacagggccg cgttggcagc tccggcggta ccgattgcaa acgtcgccac cgggatgccc
    48541 ttgggcatct gcacgatcga atgcagcgaa tcgacgccct gcaggtggcg gctggcgacc
    48601 ggcacgccaa gcaccggtaa ggtggtcttg gcagccagca tgccaggcag gtgggccgcg
    48661 cctcccgccc cggcgatgat ggccttcaat ccgcgggctg cggccgactc ggcataggcg
    48721 aacatgtcgt ccggcattcg gtgagccgag acgacgcgtg cctcgaacgc aacgccaaac
    48781 tcggtcagaa tctcggtggc cgagcgcaag gtctcccagt cgctgctcga ccccatcacc
    48841 acacccacca ctgcaggcgc gctcatggcc ttgctccgct tttttcgaag ccttgaattg
    48901 taggcggcgc ggctccatct ccggatgtgg caagacgcgc cgcgacgcac ctgacaccca
    48961 gccatgatcg acatcaccct ccagaatttc gaagccgacc tgatccacgc cagcgtcgac
    49021 cagccgatcc tgctcgacgt ctgggcgccc tggtgcggcc cgtgcaaatc cctgggtccg
    49081 gtgctcgaga agctcgaggt cgcctacgcg ggccgcttca agctcgcgaa gctgaacgcc
    49141 gacacggagc cggacattgc agggcagctg tcccagatgt tcggcgtacg cagcattccg
    49201 ctgtgcgtga tgttcaaggg cgggcagccg gtggacggtt tcgtgggcgc actggccgaa
    49261 gccgagatac gacagtttct cgacaagcac gtgccgacac cggaggaact ggaagccgag
    49321 gaggacgtgg aagccgccga agccatgctg gccgaaggag agaccgacgg ggcgatcgag
    49381 aagctgcagg aggccgtcgc gctcaatccg ggcaacgaca acgcgcgctt cgattgcctg
    49441 cgcgcactgc tgatggccgg ccgtatcgac gacgcccgcg ccacattcga tccagtggcc
    49501 ggcaagatgc tgctggatgc ccggctcgtc gcctgcggac tctggctcga tgcctgcgaa
    49561 aaggccgtgg cctcgcgctc ccccgaagta ctcgccgccg ccatcgctgc gaatcgccgc
    49621 gatttcgatg cgcgctttga gctggcgcag ctgcatttcg ccgcccagcg attcaccgac
    49681 gccatggatg agttgctgga aatcgtgatg cgcgacaagg cctggaacga agagctggcg
    49741 cgcaagacct acgtcgccgt gctgaccatg atgagcaagc cacctgccac caagtcggaa
    49801 tcgagcgcgc cgaagggcac gcttgaactg gccgggaaag tcaacaccac ccccgccgat
    49861 ccgctggtcg accagtaccg gcgcaagctg agcatggcgc tgttctgatc agtgcaacgg
    49921 acctgtcggc caggcagctt cctggtcgcg cgagctcagt gcggagttgg tttcagcgcc
    49981 cgatccagca actcgatcac tgaagtctgc atctcctggt ggcgggacca gtgatctcgc
    50041 gccagttgcc ggtagcgcag cgccgcatcg acatcttcca gcgcctggaa cacctgttcc
    50101 agccgcccgc aggccacggc cagatcttcg aggttcatcc cgggatcgag caatgcgacc
    50161 gatgcctcgg catcggcgcg cgcgcccaac gcatcgccgg tgtgccatcg gcaccatgcc
    50221 tgatcggcca ggtgcgttgc gcgcatgttc gccagcccct ggcgatcggc gtcagccagg
    50281 tgcgcctcgt agacctgcag cgccttcgcg tgctcaccct gagccgaata gaccgccgct
    50341 cgcaacatct gaacgtaggc atcctggtag atcgtgccca cccactggtc gaaattgaac
    50401 gaagccgcca cccaggcctc ggcgcggcga gcctcgtcgg tatcgacgct gccgaaaatc
    50461 gtcgccctca gcgcatgatt gctgcggtgc ccggcgatgg tgaaggtgat cacacccaaa
    50521 gccgcatcgt ccgattcggc cagtgcatgt tcacgggcgc ggatgtacca gggctgggcg
    50581 agatcaagtc ggttggcaaa gtggtaggcc gatgccgcga ccagggaagc ccttgcgtgg
    50641 gccgcgtggt ggtcaggtgc cgccagttcg aatgccagcg tcaggtactt cgccatgtcg
    50701 tccatgtcgt cctgaacgaa gctgatgtgc gcaagccagg ccgcgctcag cgcctgcagc
    50761 ggcctcagcc tggcggccgc gctcaacgcc tgcgcccgca gcatcttgtc gcgactggca
    50821 gacagccttc cgctgttctg ctcgatccat gcttcgacaa tgcagatcca cacggtgacc
    50881 agcgcgaccg gcctctgcgc aaactgactg cgcaggatcg cgagatcctc gttcgcctgg
    50941 ccgaagaacc cctgccgtgc cagaaaaccg gctcgctcgg cccgcgcgca ggccacatcg
    51001 atcgggtgca ccgacgccgc aagcgccgca tcgagccgcg ccagcatcgg cgaagctggt
    51061 gccttcatgc ggtcagacgt tgcagcgctt cccggtactt ggcggcggtg gcgtccacca
    51121 ccgtcgccgg cagcgcggga gccggcgcct gcttgttcca gggagcgccg ttcaccacgg
    51181 cctgttcgag ccagtcgcgc acgaactgct tgtcgtagct cggcgggttg agtcccgcac
    51241 tgaacgccgc ctcatagcct tccagaggcc agaaccgcga cgagtcgggg gtcagcacct
    51301 catccatcag ggtcaaggtg ccatcgacgt ccaggccgaa ttcgaacttc gtgtccgcaa
    51361 tgatgatgcc cttggtcagc gcgaactcgg ctgcactgcg gtagagctcg atggccacgt
    51421 cccgcacctg cgcggccagt ggggccccga tgatctcgac catgcgctcg aacgagatgt
    51481 tctcgtcgtg atcgcccatc tcggccttgg tcgcgggcgt gaagatcggc tgcggcagct
    51541 tcgacgcatt gaccagtccc tcgggcagcg cgacgccaca caccgagcgg ctttgctggt
    51601 attccttcca gccgctgccc gcgaggtagc cgcgcaccac ggcctcgacc ggcagcggct
    51661 tcaggcgctt gacgagcatg gatcgaccga cgacctcggc gcgctcatcg gcggcaacga
    51721 cgctctcggg tgcgcccccg gtgaggtggt tcggcacgat gtccttcagg cgctcaaacc
    51781 agaacagcgc catctcggtc agcagctggc ccttgccggg aatgggctcg cccatcacca
    51841 cgtcgaaggc gctgaggcga tcgctcgcga tcatcagcaa acggtcggtt ccaacggcgt
    51901 agttgtcgcg caccttgccg cgcgccagca agggcaggga gtgcagtcgg ctttcgagca
    51961 gggcttgggt catcgcgagg cttgttcgtc ccggtggttt agaggcgagg tcagaccacg
    52021 gtctgcgcca gttcaccctt ggcgtagcgc gccgccaccg cggccagcgt ctgcggcttg
    52081 atcttcgcgg cctgtccagc gcaattgaac tcgaggtagc gctgcttgca gatctgcttg
    52141 gctgcctcgc gcgccggctt caggaagtcg cgcgggtcga acttgtcggg gttctcgaac
    52201 aggtacttcc gggttgcggc ggtcattgcc agacgcacgt cggtgtcgat gttgatcttg
    52261 cgcacaccga acttgatggc ctcctggatc tcgctgaccg ggacgccata ggtctccttc
    52321 atctggccac cgtactggcg aatgatcgcc agcaggtcct gcgggacgct cgagctgcca
    52381 tgcatcacca gatgggtgtt cggaatgcgc ttgttgatct gcttgatccg ctcgatcgcc
    52441 aggatgtcgc cggtgggctt gcgggtgaac ttgtaggcgc cgtggctggt accgatggca
    52501 atcgccagcg cgtcgagctg tgtcttcttg acgaagtcgg ccgcctcttc gggatcggtc
    52561 agcagttgat ccctggtcat cgtcgaatcg gtgccgtggc cgtcttcctt gtcgcccttc
    52621 atcgtttcca gcgatcccag gcagccgagt tcgccttcga cggtgatgcc gagcttgtgc
    52681 gccatctcga ccaccttgcg cgtgacgtcg acgttgtatt cgtagctggc gatcgtcttg
    52741 ccgtcggcct gcagggagcc gtccatcatc accgaactga agccgagatt catggcgccc
    52801 tggcagacgt cagggctctg accgtgatcc tggtgcatca ccagcggaat gtgcggccag
    52861 ctttcgacag ccgcctggat caggtgcttg atgaacggtt cgcccgcgta tttccgcgcc
    52921 ccggcgctgg cctgcaggat gaccggcgca cccacctcgt cggcggcgga catcactgcc
    52981 tgcacctgct cgaggttgtt cacgttgaag gccgggatgc cgtaggagtt ttccgcggca
    53041 tggtcgagca attggcgcat cgagacgaga ggcatggtcg tatccagtgg agttgtggaa
    53101 cccgagaggt gcgcctgcga gccgtcggca acgcggccgc cagaacagct gcggcaggag
    53161 gccccctgca atccctacat tgtatcgacg cctgtgctgt ggctgcccgg caggcgcccg
    53221 ccccgaaatg cgcgcgaaag cccgtgcttt tcgggcggaa ggcgtgccgt gcaggggtca
    53281 cactggactt gtaagcgctt gttcaatgat cacgctggag ctgaccatgg cccgtatcga
    53341 tctgaccgca ctgaccacct ggatcactgc cgccgcccac gaccatcccc gggacctgcc
    53401 ggatatgctc gccgggctgg ctggcgttca gcgccagacc gccgtgcggc gcctcggcga
    53461 attgatcgac ttgcagtggc tgactcgcac aggacacgga aaggacgttc gctacgcccc
    53521 cggcctgctg cgccagatcg tgcggcacta tgcgctcgac ggcctcgcgg aagaccttcc
    53581 ctggtcgcat gacttcgcgc gctacttcgc gttgccaccc aatgtgcagc gtctgaccca
    53641 gcacgcattc tgcgaactgc tcaacaacgc catcgaccac agcgacggca ccggcgtcac
    53701 cgtctcgctg cgacagactc cgagccacgt ccagttgctg gtgtcggacg acggctgcgg
    53761 ggtgttcgac aagatctgcc acacgttcgc gctggaagat ccgcagaccg cgatgctcga
    53821 gttgagcaag ggcaagctga cgacgcagcc agagcgtcac accggccacg gcctgtactt
    53881 cacttcccgg ctggccgacg tgttcgacct gcatgccaac gaccatgcgt tccagcaccg
    53941 cagctgggag ggcacgggct ggcgcccggg tcggccgatg aaccggctgg gaacctcggt
    54001 gtacttgtcg atcgctctcg acacgcctcg cacactggaa tccgtgatga acgcgcacag
    54061 cctggacggt accggcgttc gtttcgaacg caccgccgtc ccgctgcagc tcgcggcaac
    54121 accggaggcc gggctcgaat cgcgcgcgca ggcccgcctg gtggccgcga ggctgacacg
    54181 tttcaggctc gcggagctcg acttcagcgg tatcccgcac atcggacaca gcttcgcaga
    54241 cgaattgttc cgtgtgctgc ctcagcatca gccgacgctg aacctgtcac cgatcaacat
    54301 gagcccggct gtcgccgcga tggtcgacag catcgtcacc gcctgagaaa actcaggcga
    54361 cccggcagac cttcagcatg ttggtgccgc ccgggtagcc catcggctca ccgacggtga
    54421 tcgcataggt atcgccgggc ttcagcacac cgttctcgac cagcagtgct tcggcacgtg
    54481 caagtgcctg atcacggtcg gtgcagctgg ccatcagcag tgggcgcacg ttgcgataca
    54541 gggccatctt gcgctggctg ttgacctgcg aggtgagccc gtagatcggc acatggacct
    54601 tgtgccggct catccacaga gcggtcgaac cggactcggt cagcgcgagg atcgccttgc
    54661 accccaggtg gtgcgccgtg aacagcgcgg ccatcgcgat cgactggtcg atgcggccga
    54721 agtgcttgtt ggtgaagccg gcatcgagcg agatctcctc ggcgcgctcg gcctcgatgc
    54781 agatcgtcga catcatctcg acggtctcga ccgggtactt gccggccgcc gtctcggcgc
    54841 tgagcatcac ggcatcggtg ccatcgagca ccgcattggc cacgtcgctg acctcggccc
    54901 gggtgggcac gggattgacg atcatcgact ccatcatctg cgtcgcggtg atcacgatgc
    54961 ggtcgagctc gcgcgccatc ttgatcatct tcttctgcag cgccggcacg gtggcgttgc
    55021 cgacctcgac ggccagatct ccgcgcgcga ccatgatgcc gtcgctggcc ttgaggatgc
    55081 tttcgagcac ggggatcgcc tcgctgcgct cgatcttcgc gatcaacgcg gccttgtgct
    55141 tgaaaggctc gccggccacg ttggcgagct gtcgcgccat ttccatgtcg gttgcattct
    55201 ttggaaaact caccgccagg tactcgcact ggaagctcat cgcggtcttg atgtcttcca
    55261 tgtccttgcc ggtcaaggca ggcgcggtca ggccaccgcc ggccttgttg atgcccttgt
    55321 tgttcgacag ttcgccaccg acaacgacgg tggtgtgcac ctcggccccg cggaccgcgt
    55381 cgacagtgag cttgagcaat ccgtcgttca gcagcagggt gtcaccgggt cgcacatcgc
    55441 gcggcaattc cttgtagtcg aggccgaccc gctcgttggt gccgagctcg gtggtttctc
    55501 cgtcgaggat gaacgactgc cccgcttcca gcatcgtctt gccgccgtcg aacttgccga
    55561 cacggatctt cgggccctgc aggtcggcca tgatcgcgac ctccttgccg acccgtttcg
    55621 ccacttcccg caccatcgcc gcgcgttcga tgtggtcctg ggccttgccg tgcgaaaagt
    55681 tcagccgcat gacatcgacg ccggcgagga tcatccgctc gagcacctcg ggtgagctgg
    55741 acgcaggccc cagtgtggcg acgatcttgg tggcacgagg catgaaagga atctccggga
    55801 gggtctggct gggaaaagat cagcgcaccg gcggttgcgg cgtcgccgtg tcggggcccg
    55861 gcttcgggcg ccgctgttca ggacatcgcg ccacgcgccc gctgctccag gatctcgatg
    55921 gcgggcaaca ccttgccttc gagcatttcc aggaaagcac cgccaccggt gcagatgtag
    55981 cccacgccgg cctcgacgcc gaacttcgca atggctgcga gcgtatcgcc gccgccggca
    56041 atactgaacg cgtcggatgc ggcgatggca tgtgcaattg cctcggtgcc gtgcgagaac
    56101 gcctcgaact cgaagacacc taccgggccg ttccagacga tggtgccggc cgatttcagc
    56161 ttctcggcaa gcactgccgc ggtcttcggc ccgatgtcca ggatcatgtc gtcgtcggcc
    56221 acgtctgcgg ccgccttggt ggtcgccgtt gcgtctgccg caaagctctt cgcggtgacg
    56281 acgtcgaccg gtatcggcac ctcggcaccg cgcgccttca tcgacgagat gacggccttc
    56341 gccttgtcga ccagatcggc ctcggccagc gactttccga tgggcaggcc cgcggccagc
    56401 atgaacgtgt tggcgatgcc gcccccgacg atcagcccgt cgactttggc ggagagtgcc
    56461 tcgagtatcg tcagcttggt gctgaccttg ctcccggcaa cgatcgcgat cagcgggcgc
    56521 ttcggttcgg ccagcacctt cgtgatcgcg tcggcctcgc cggccagcag cgggccggca
    56581 caggcgaccg gcgcgtactg cgcgataccg tgggtggagg catgcgcgcg gtgcgcagca
    56641 ccgaacgcgt cgttgacgta gatgtcgcac agcgcggcca tcttgcgact gagcgcttcg
    56701 ctgttcttcg attcaccgac gttcagccgg cagttctcga ggagcaccag atcgccggag
    56761 gcgagtgcgc gtccatcgac cgtcacgcca tcgacccagt cggcaatcag tggcaccttg
    56821 cggccgagca gcttgccgac atgctcggca atcggtgaga gcgagtcttc agccgtccac
    56881 tggccttcct tcggccgacc gagatgcgaa gtgaccatca ccgcagcgcc cgccttcagc
    56941 gctgcttcga tgcacggcaa cgaggcacgg atgcgggtgt cgtcggtgat gcgaccatcg
    57001 gcgtcctgag gcacgttgaa atcgacgcgg atgaagacac gcttgccagc gacttggttg
    57061 gcggcgatga gatcggtgaa tctgatggcg ttcatggtga gtcggaggga tgggattttc
    57121 gagtttaaag gtgcccgctc ggcggctggg cggtgcttac caaccccaca gcagccgcaa
    57181 tggcagaaac acggcgaggc ccgccagaag cggcccgagc acgccgggtc gccaggcata
    57241 ccagacactg gccgccagcg cggctggcag ccgcgcatcc tgccaggagt cgatcagtcg
    57301 cccctgcgtc atcacgatct ccggcacgat gaccgcggtg agcgcggcca gcggcgcgac
    57361 cttgaggccg cgcctgagcc aatcgggtat cggcacctcg cgctcgggca gcagaaagaa
    57421 gcttcgcgtg atcacggtga cgatggccag cccggcaacg gtcagcgcga catacgcggc
    57481 atcgcccgaa ccattcatct tgtgttcccc cgcaatcgcc gactggcctt gtcagcctga
    57541 tccatcagca ggccggcggc caccgccgag gcaatcgcga cgatgatgtt cagcttgagc
    57601 ggcagcgaaa acgcggcaac ggctgcggtg ccggcgacaa ccgctgccac ccaggtggat
    57661 cggtcggaca acaacccgta ggtcagaccc accatcgcca gcgtgcctgc gaatcccaga
    57721 ccccaggcgg tggggacgag gttcgccaaa gcaatgccca gcatcgacgg cccctgccac
    57781 acgctccaca ggaccgccac tccgcccagg tagtacggga tctggcccgg ctcccgacgc
    57841 gggccctggg gcccggacgg ataggcgcgc tggaaggcga tcaggttcag atcggccgca
    57901 aggtacccga atgccatgcg ctggcgcagc ggaaggtgcc cgaagtaggg cctccactgt
    57961 gcgctataga tgacaaagcg caagttgacg cagaacgcag cagcccagat cacccacatc
    58021 ggcgcgccag ctgccagcaa cggcaaggca gccagttgcg cactgcctgc gtagacggcg
    58081 aacgacatga gcacggccag cggcacgctc agccccccct tgaccatggc cacaccggtg
    58141 accattcccc aggcaccgat gcccggtgcc atggcaagca tgtcgcgggc accgcgtcga
    58201 aagtcggggt cgcctagcaa ccgggccgct ggattcatcg aaccattgtc ggggcttgcg
    58261 tcccgcaggc aggtggagca cacgctcctg ccagcgggtt gcgaggaagc ccgcccggtt
    58321 cgctatccgg cctgggtcgt cagtgcgcct tgggctcttt cgcgtccttt cccgccttgg
    58381 ctggcttggc gggcggggcc tcggcatcga cggattcggc gtccgtcgtc ttggcgctgg
    58441 caacttcggt ggtcagctgc ggcggcaccg gttcgatctt gttgtcacgc atgtaatcgt
    58501 tcatctcgcg ccagcccgcg aacaccgcag ccttgtcggc acgcttgttc aacgcatagc
    58561 agtcctcgat cgcgcgaccg gcatgacgac aggcgccgcc gacagcactt ccttctgcct
    58621 gctttgccgc cgcaatcttt tcgggcgatt cgatgcccag ctgctcgcag ccagtcagca
    58681 agagggcggc aacggaaatc agcgcagaca cgacaggacg caacatggtg gatgtcttcg
    58741 gcagtgcgaa agagtctttg gacctgttcg gtatatcggc gccaacgaag acgacttgat
    58801 agccgctcga accccggccg gggcgacttt caggttgcga cagcttccag gcggtcgccg
    58861 acctgcacgg aatgcccctg caggaactgc gcagcagcca tccgcttgcc gccaggccgc
    58921 tgcatctcgg tcaggcgcac cgcaccggat ccacaggcca ccgcgatgcc atcggccgac
    58981 acagccacca cggtgccggg caccccacgg cccgcgtcgg aagcgttgat tgcctgagcg
    59041 cgccacacct tgagcggcgc gccgcgccac tgcgtcaccg cgcccggcgc cgggttgaat
    59101 gcgcggatgt gctgcgcgac acggcacgca tcggcggccc agtcgatggg tgcctccgcc
    59161 ttgtcgacct tttttgcgta agtgatgccg tccgtcggct gcggcgtcgg gcgcaaaagc
    59221 ccacgagcag cctgctcgag cgcctcgaca atcaaccgac cgcccagaac cgcaagccgg
    59281 tcgtgaagcg agccggtggt gtcgtcgtcg aaaatgtcca ggggctctgc catgagcatc
    59341 ggcccggtgt cgaggcccgc gtccatctgc atcacggtga tgcccgtgcg ttcatcgccc
    59401 gcctcgatcg cacggtgtat cggcgcagct ccgcgccagc gcggcagcag cgacgcatgg
    59461 atgttcagac agcccaggcg aggcaagtgg agggtccatt cgggaagaat caatccgtag
    59521 gcggccacga ccatcacatc gatccgggcc gcctgcagcg aatcctgcgc agccgatgcg
    59581 tcgtcggcaa agcggccgtc caggcgcagc ccctgcggct ggatcaccgt caacccgcgc
    59641 tcgactgccc atcccttgac cgcagacggc tgcggctgct ggccgcgccc ggaggggcgg
    59701 tcgggctgcg tcagcacgag tgcgacctcg aacccagatt ggtgcagcgc cgccaaggca
    59761 acgcgggcga attcaggcgt gccggcgaat gccagcctca tggacgctga gccagaaatt
    59821 tcacaaagta ccatgaacgg cgctcaacgc ccctcatcac gcgcctgttt gagcattttc
    59881 gtcttgatgc ggttgcgctt cagcgggctc aggtagtcca cgaatacctt ccccatcagg
    59941 tggtccattt cgtgttgcac gcactttgcc agcagaccct cagcgtcgaa ctccaccacc
    60001 tcgccatcac gaccgagcgc tcgcacccga acccgggccg gtcgcagtac cttgtcgtag
    60061 accgttggca ccgaaaggca cccctcctcg cccagcacct gctcgtcgct tgcgctgacc
    60121 aactccggat tgatcagaac ccgctggtcg ttgtgctggt ccgacgtatc gatcacgatc
    60181 aatcgttcat ggacatcgac ctgggtcgcc gcgagaccca cgccttcagc ggcatacatg
    60241 gtctcgaaca tgtcgtcgat caaccgcctg atgcggtcgt cgacctgctc gatgggcctg
    60301 gctgtggtgt gcaagcgggg gtcggggaag cgaagaatcg tgagcttcga catggtgtgg
    60361 tgctatagac ggcggcgcgg ccggtcaagg ccacccggcg gaacagggat gtggattcgc
    60421 tacaacgcaa ggccctgtac tgacttgctt cagaggcgca gtaaggccct actttcgttg
    60481 cgatatcaag ggtttagggg cagaatcttc gaaacagttt gaagattttc gctgaatttc
    60541 cggccgcgtc ggccgatacg gatatgtgag tattcggcac ccctcccccg gccaacccat
    60601 gacgcgacgc tgcgagcgtc ccgtccggag atttcacccc atgacgcgtc ccattttttc
    60661 cgcgcttcgt cgccggccgc ttgcagccgc cctcggcctt ggtctggttc tgactttcag
    60721 ctggactgtg ccatcggtgg cagctccgaa cttccccgtg accgagcaac aacgaggcac
    60781 ggcccagaaa gtggcatcgg ccggcgtcgc attgtccgaa ctggcgccca acgcaccgga
    60841 atcgcacacc gtcgttcgcg gcgacaccct ttggggcatc tcgagcctgt tcctgaagtc
    60901 tccctggcgc tggccggagc tgtgggggat gaacatcgat cagatccgca acccgcacct
    60961 gatctatccg ggccagacgc tctatctcga cacctccgac ggacgcgcac ggttgcgact
    61021 gggcaaggcg atcgaaggca atgtcgtgaa gctgaagcca acggtgcgca gttcgcccct
    61081 gtcgaatgca gcgatcgcgt caatcccgtt cagcctgatc gagccgtttc tgaacgaagc
    61141 cgtgatcttc gagggcaacg tgctggatgc agcgccccgc atcgtggcca cgcaggaggg
    61201 ccgagtgctc ctcacacgcg gcgacaaggc ctacgtaagg ggtgaaatcg gcaaggacac
    61261 cgtgttccgc atcttccgag aggcaaagcc actcaaggac ccgacgacgc aggagattct
    61321 cgggttcgaa gccccctatg ttggtacagc cgagttcgtt cgctcgggag tcaacagccg
    61381 ggtggccagt tcgagctccg ccgaaacggt gcccgacacg ttcatcgtga ccagtgttcg
    61441 ggaagaagcg aacatcggcg accggcttgc cccggttacc ggacgcgaat acgacaacta
    61501 cgccccgcac gccccccagg cacctttgtc cgggcagatc gtctcggtct acggcacggc
    61561 ggtgtccgcg ggtcagaacc agatcgtcac cctcaaccgc ggaaagagcg acggcgtcga
    61621 gcgcggccac gtcctggcct tgtggcacga cggcagcaag gcgatcgacc gcacggacag
    61681 ccggctcacc gaattgaccc tgcccgatga gcggcacggc gtcctcttcg tgttcagggt
    61741 gtttgagcgc atgtcatacg ctctgatcct gtcggtgcag gaaccagtgc gcatcggcga
    61801 caagttcacc cagccctgac cgggtagctg ccggtggcgt cgtgatggaa cgcgacgagc
    61861 tggcagcttg gctgcggttg ctcggaaccc cggcgctggg tcgagcatca gcgcgtcgat
    61921 tgctgtcaag cttcggctcc ccccaggctg cagtccatgc aacggtgcat gcgcgccggg
    61981 aggtgatcgc cgaagccgcg gcgtcagcac tggcgacacc ccctcccgac ctggaatcct
    62041 cggtcgatgc gacgctggcg tggctggggg atggtgccgt cggccctggt gcagcgcgca
    62101 gggagatcat cacgctgggt gatcctgcgt atccgtccct gctactcgag accgcggacc
    62161 caccactgct gctttacgcg cagggccgcc tcgagctgct ggcggcacag ggcatcgcga
    62221 ttgtcggcag tcgcaacccc acaccgcagg gagagcagga cgcgcgagcc ttcgcgaaga
    62281 atctcagcag cgcaggctgg gtcgtcgtgt ccggactggc tctgggcgtc gacggtgcgg
    62341 cccacgaagg tgccctcgaa ggtgcggccg gcacgatcgc cttcgtcgga actggtctcg
    62401 accgggtgta cccgaggcgc cacctcgagt tggcccatcg aatcgccctg aacggactga
    62461 tgctcagcga gttccccctg ggcacccctc cgctggcggc acactttcct ttgcgcaacc
    62521 ggttgatcgc ggggatgact cggggcacct tggtgatcga ggccgccctg cagtcgggat
    62581 cactgatcac tgcgcgcctc gccagcgagg cgggtcgcga ggttttcgcc atcccgggtt
    62641 cgatccactc gccgcagtca aggggctgcc acgcgctgat caggcaaggc gccaagctgg
    62701 tggagtcggc cgaagacgtg ctggaagagc tgcaaccggg agcatcggtg cttgaacggt
    62761 cagccttcgc aacatcgtcg tccgcaaaca cagacccgct gctggatgcg atgggtcatg
    62821 acctggtgtc gctcgatgcc ctggtcgcgc gaaccggctg gccagcacag gaactgaatg
    62881 tccgcctgct cgaactggag ctgagcggcc agatcgcacg tcgccccgga cagctgttcc
    62941 agcgcgtcac gctcgcctga ttcagagcgc acgcagcggg tcgcatgcga cccgaaattc
    63001 cctgcctgga gacgcatctg ccgcccgatc tgctcgggta tgatggattc atgttcgatg
    63061 tgcttgtcta cttgtacgag aactactggc gcccagatgc ctgtccggat catgcgcagt
    63121 tgactcgaaa gctctcgtcg gtcggcttcg aatccgacga gatccaagag gcgctgtcct
    63181 ggcttgatgg cttggcaagc gcggctgaat cgtatgtggg cgagcaggga gaacacagtc
    63241 tgcgtgtcta ctcgcccgcc gaacaggaac acctcggcga agcctcgata ggcttcatca
    63301 gctttctcga atccgccggc gtcctgcctc ccccgatgcg cgaaatggtg ctcgatcgcg
    63361 ccagcgcgat cccaggcggg cctctcgatc tggaagacct gaagatcatc gtgctgatgg
    63421 tgttctggag cctcggggag gagcctgacg cgctgatcct ggacgagctg ttcgtcgacg
    63481 aggaagaccg cttgatccac tgaatcgatc gcgcgagctg atcgctcgct gcgaactgaa
    63541 gaagccgcca agaggcggct tctttcatgg cggatacgga cacgccccca ggttcagttc
    63601 agcaaccgac ccggatccac atcgagtttg gccagcgcgc tacgcgtggc tcgcactgtc
    63661 agagactcgt cctgaacagg caccagaagc cccgcctgca ggaaggcagc aagacgttgc
    63721 tgctggaaca ggatgccgcg cccctgtggc gaaacgaaca gtgaaagctg ccgacgcatg
    63781 ccttgccagg ccaattgaac cgactcgtta cggtcccggt aatcgagcat gtaccaactg
    63841 ccgacctgca gctcgcgcgc ccacgcaacc atggccggcg tcggcatcga accacccacg
    63901 gagacaactt ccagttcgct gctttcatgc cccgagaggt cgagcatcag agactcatcg
    63961 atatcgacgt cttcggcatc aggaagcagc tcttccaggg tctcgagccg ctccatcaac
    64021 tgttcgaggc gctgcttggg gatcgatgcg gtacgcgcgg tgaaggcggc cgcaagcgaa
    64081 ttgttcagcc gctgaatgtg ctcatcctgc tgcccgaggc tgacacccgc gctctgcatg
    64141 ccgtcgcgca ggatcttcag cagtggtggc aggcgccgga tcacatctgc acgctcttcg
    64201 cgcgagacct ttgcgctggc agaccagatg agatccgcag ccgtgcgctt catcgtcttg
    64261 atctcgtcgc tctgcagccc ggacttgacc gcggtcatcg caaggacgtc ggcccacaca
    64321 tggaacagga actcgcgcac gccttcctgc acgggcatct cgttgagcat cttgcgcaac
    64381 tcgatcgtgt actggatggc gagagtttct cgctgctcga cctgctgcgc aagggacacg
    64441 cccttgcgag aggcttcgtt ctcgttcttg aagtagtgct cgagaaagcg ttcgaactcg
    64501 gtgagcaccg tctggaacac gcggcggcct gtatcggggt aggcctcgac aacctgcacg
    64561 acacgcttga tttccttctc taccgcaccg ccggaaaggc gggtgctgtt ggcatcgaac
    64621 cccatcacac aggcacccat ccgatcgatc agacgtcgtg ccggatggtc ggtcgtggcg
    64681 aagaaatcgg gctcactgac tgcgacgcgc aacacaggca tctgcaggcg tgcgaaccag
    64741 acacgcaccg acgccggaat gcgctcctcg gtcaggatgc tctggaacag cagtgccacc
    64801 acctcgatcg tcgcgcgctc accgggagaa gaagtcgccg ccttcagcgc ctgcttgcgc
    64861 tgatgcagtt cctcgagcat cgccggcgtg ctgatcggag cgctggcgtg tcgcgcacca
    64921 ggttccggcg tgagcctcaa aacgatgccc tgctgggcgc tggtgatcgc cgcatcgagc
    64981 cccggagaag ccagatgccc gtgcgcggtg cgcgcaaagt cgggcagctg ccgtccgatg
    65041 agtcgattca gccggcccag cacggcttcg gcatgatcac tgccgccacg cccgagtccg
    65101 ccagcgcggg tcatcatccg cgtctcttct ccgacgctct tgtcgtcggc tactgcgcgg
    65161 cctgcaccct cgagcgcggc aacgggctgg ccagtcagtt cggctgaggc gagtgcgccc
    65221 aatccagttg ccgggccggc ggcgagtgaa cccgattgcg tgttgacgaa ggcctggttg
    65281 cgcgagcgac gaatcaacgg acgcagatcg atttcgggca acaccccctg ctggatcagc
    65341 agccggtttg cctcgtgata ggcctcttcg ttgaagtgcg cgaactcctc gtgcagcacg
    65401 ccttgcaggg tgcgccaggc ttcgtggctg agccccgcgg tgcgccagga agtgatggcg
    65461 atgcgcgcca gcacatgcgg ccgcaggatg tcattggcgt cgagttcctc gcggccttcg
    65521 agcatcgtca ttctcgagcg caggtccgtg aactcccatg aagcgcggtc catcacggca
    65581 agcgcaagac gagaactgag gatctcgccc tcgatggttt cgttatcgac caggctgaaa
    65641 ttctggcgac ctccggccat cggcagatcg ttggggcgcg atgccgacac caacccactg
    65701 tgaagcgcgc ctcgaagcat cgacatcgca ccctgcagcc agaaaccgga cgcattcttg
    65761 atgtcctcga ctgcatcgcg tcggcgcatc atcgtggctg ggtcgctgat ctggctcagc
    65821 aacaggcggg cgccgtgatc cactgcatgg acgacacccg gcagcccttc gatcaggccc
    65881 tcgacatacg aacggcgggc ctgggtggcc aaggccatcg tttgggcggt ggacgccatg
    65941 ggttcgagct gccttcgggg gtggatgcgt tttgactcta caccaccgct ccggcgttcg
    66001 gatcatcggg gtcgagctct ttgctggtcg gcttgatgag atcgtcgcgc ttgacgccca
    66061 gccacattgc gatggctgct gccacgaaca ccgatgaata gatgccgaac aggatgccga
    66121 tggtcaacgc gactgcaaag tagtgaagcg tcggcccccc gaagatcagc atcgacacca
    66181 ccatcatcaa ggtgcaaccg tgggtgatga tcgtgcggct gatggtgctg gtaatcgcgt
    66241 ggtcaatcac ctgcggggta gtcatcttgc ggtacttgcg gaaagattct cggattcggt
    66301 cgaagatgac cacggactcg ttgaccgaat agcccaggac cgcaagaacc gccgccaaca
    66361 ccgacaacga gaactcccac tggaagaacg cgaagaagcc gacgatgatg atcacgtcgt
    66421 gaaggttcgc aatgatgccg gcgaccgaga atttccactc gaatcgcacc gccaagtaca
    66481 tcatgatgcc ggcgatcgtg accgccagcg ccagcgcccc gtcacgcgcc aattcagaac
    66541 cgacggacgg cccgacgaac tcgctgcgct gcagcttcac cttctcggcc cccacctcgc
    66601 cacacaccgg ggcagaggat tcggcaccct tgtccgactt gatgagctgg gtcgtgaccg
    66661 tgccgccttc tgccttgcac aacgctgcga agacgcgatc gaccacctcg gcctgcttga
    66721 catcagcgcg caccggcagt cggatcaaca catcgcgcga ggtcccgaaa ttctgcacct
    66781 gcacctcgcc gaaccgcagc gattcgacgg tggtgcgcac ccggttgatg tcggcctgct
    66841 gcgcgtaggc cacctcgatc acggtgccgc cggtgaattc gatcgagaaa tgcaatccgc
    66901 gcgtcgcgat gaagaacacc gccgcaacga aagtcagcgc cgaaatcacg ttgagcacca
    66961 gcgcgtggcg catgaacggg atgtcacgcc tgatcctgaa aaactccatc ttgaattcct
    67021 ttgcttggag tcttcagaac cggctcacag ctgcgcaaga cgcaggcgat gccgaagcca
    67081 aagattccat ccgcgttcct tgttctgcct cgggctgcgc tcagcccttc gcgcccgcat
    67141 cggaagccgg cttccagacc tggccgatcg acacgccctt gagcttcttc tgacgcccgt
    67201 accagaggtt gaccaggccg cgcgagaaca tcaccgccga gaacatcgag gtcaggatgc
    67261 ccaggcagt
//
LOCUS       DAOORV010000005        65925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_455_length_65925_cov_9.548717, whole genome shotgun sequence.
ACCESSION   DAOORV010000005 DAOORV010000000
VERSION     DAOORV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 65925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65925
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_455_length_65925_cov_9.548717"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKV56_01345"
     CDS             <1..536
                     /locus_tag="PKV56_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX66479.1"
                     /translation="AQQGLTEKDVTLVNMAAPDAGAAFAAKSINAAVTWEPFGTQGIK
                     SGGKLLLTSKDTPGAIVDVLGVGNDVLKNRPDAVKLLVASWFDALEYVKKHQAESFKI
                     MAKASGVSVAEFTEMWKGVRIPTLAENQTALGTAPGSGSYRKTVESMSSFMIEQKLIT
                     QPVAADSMVSGAFLPAK"
     gene            complement(551..1399)
                     /locus_tag="PKV56_01350"
     CDS             complement(551..1399)
                     /locus_tag="PKV56_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOX66480.1"
                     /translation="MNSAAALDPSPVRPALCFDDVNLDATELHDEPAPHFTLCLVLQG
                     EARLSHRIDDRWHTDWLRPGMFAPITPPHAGATLHLSQAQRHLMISIGADAVARIAAE
                     TGQAVEQLEPLQARSFKDPFLSDLCRRAWVETRATDALGRRFARSAEEALICGLLRVA
                     SATGDSPRRRPPAALPAAMLKRIHDCCIEHLNEPLEVADLAAIAGMGPQRFGAAFKAA
                     TGRSPQQYLIGLRVDQARDLLVNSRLVIAEIALRCGFFDQSHLTTTFTRRVGVPPLRY
                     RRQATR"
     gene            1695..2093
                     /locus_tag="PKV56_01355"
     CDS             1695..2093
                     /locus_tag="PKV56_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66481.1"
                     /translation="MNKLWIGLMGAALSASAAAEWTEIGGNESITFYADVATLDSSGG
                     TKKMWTLVGSKSERKYGEIKFSSIKTQFEFDCPAQKVRQLETAFYAGPLADGERVAGY
                     ADTGDWEPIVEGTVKDGLAKIACETAVPKS"
     gene            complement(2122..3366)
                     /locus_tag="PKV56_01360"
     CDS             complement(2122..3366)
                     /locus_tag="PKV56_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX66482.1"
                     /translation="MHILISCVGSAGDVHPFIAIGTALAQRGHDVELLTSPHFRTRIE
                     AAGLGFTPVGTEADYQRVVQQAALWHPRRSFPVLWQEMQPRLLEAHRSLLSRVQPGRT
                     MLVGSTLAWPVRLAQETHGLPAVTVHLSPICMFSAQAPAVLPGVGDLSGWPAPLVRLI
                     HSSVERGFIDRVIVPGFDPIRRALGLPPVRRVMSHWVNSPQQVVCAWPEWFAPHQADW
                     PTQAVTTGFPRWSAADASLEPTLVEFLEGGTAPIGFTPGSAMAHGRDFFARALAACAA
                     LNRRALLITPYADQLPQPLPPFAQAVGYAPFDLLLPRLAALVHHGGIGTTAQALAAGV
                     PQGLVPHAHDQFDNATRLVKRGVGVRLPARGSARRWSQAIDRLINDPAIAAACRRDSA
                     LMAQEGDAAQRIADLIERHSRD"
     gene            3421..4473
                     /locus_tag="PKV56_01365"
     CDS             3421..4473
                     /locus_tag="PKV56_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX66483.1"
                     /translation="MRIDQIRQRLRAHGALPCHEHRVLRLWSNALSQDSGRRKPQDFL
                     PLGVRAELAALETDLQGLARLISEHPADDGSARLLVGLHDGQTVESVLLPRDGLCVST
                     QVGCAVGCVFCMTGRDGLLRQLGSAEIVAQVALARSLRPVKKVVLMGMGEPAHNLDNV
                     MDAIELLGTEGGIGHKNLVFSTVGDRRAFERLGQGPVKPALALSLHTTKAALRARLLP
                     RAPAIEPDELVALAEGYAQATGYPVQVQWTLLAGINDGDDELDGIVRLLAGKRAILNM
                     IPYNDVDGLDFQRPLAERAREIAVTLHRRGVLTKLRQSAGQDIDGGCGQLRARQAPDP
                     AHVPDDRLRIVAVRPR"
     gene            complement(4480..6363)
                     /locus_tag="PKV56_01370"
     CDS             complement(4480..6363)
                     /locus_tag="PKV56_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOX66484.1"
                     /translation="MMKPIQASHSAPRHRGAATLLALGIAALLPGCALQTPLRSPNAG
                     DTVRDELLAARSGTPARAASPASAATVEAPAAASPSPLAAAPAPRPETPPEPRFDLSV
                     SGAAARDVFLAMVSDSRYSMLMHPDVGGTLSVMLRGVTVREALESIRDVYGYDFRIDG
                     RRITVYPPTLQTRIFMANYLQAKRQGRSDVRVSSGSAPVTNNGNNNYNNSGNSSGSPN
                     GSNQGNGGASLDSSQISTTSSSDVWAETSEALRSIVGNANGRNVITSPQSGTIAVRAM
                     PEELRQVEAYLRASRLAIERQVMLEAKIVEVELREGFQSGIDWSILRSRGSIGQTSGN
                     AGNALVSNSKGIPTLPSAVSSLLIDHVSLPTAGAGTLGLALATNGFQAVLGFLESHGD
                     VQILSSPRVATLNNQKAVLKVGTDEYFVTNVSGGTITAGNNNNNGTTTLPTLTLTPFF
                     SGIALDVTPQIDEANTITLHVHPSVTAVTEKTKQIDLGTIGSYKLPLASSSVNETDTV
                     VRVTDGNIVAIGGLMQVEANGRNSGLPGSSNNVVTRALFSNNDVSSRKKELVVLIKPT
                     IIRNVEDWQQANQQTGSLFDESPDTPRRVISVKPAPTATEGAPAATVAAAGIRPAAQA
                     EAR"
     gene            complement(6360..6833)
                     /locus_tag="PKV56_01375"
     CDS             complement(6360..6833)
                     /locus_tag="PKV56_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66485.1"
                     /translation="MKRLAASRQAWLALAAAAMSLAVHAQGIDAQTMRDPTVPPAAAR
                     PTAATPNAPGGVAAPPSTPKHLVRIDGVPYVVEGTRRRGVGDLLGSARIERITETDVW
                     VSEGGSVTRIPLHGNVVKQVAVESAAAASDSRPARKACKNPKHRCSQTLIRGESP"
     gene            complement(6830..7477)
                     /gene="gspM"
                     /locus_tag="PKV56_01380"
     CDS             complement(6830..7477)
                     /gene="gspM"
                     /locus_tag="PKV56_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOX66486.1"
                     /translation="MKPLWKQHWRRIEALSLRERLMIVVSVMFVMGAIADTLVLAPAK
                     AQQNALRAEAATQARELDALRQKLNSAARDAGSDGPRDSLQQRLARSIDETKNVDRQI
                     GERLGDAGRPARLPELVDQVLRRNDRLTLTRLITVAERPGEKSAAKGKDAPAAASLQW
                     QAVDLGVSGSYLDIVQYLADLERALPELRWGPLKIHAEQTPPELSVRLFLAGEVK"
     gene            complement(7474..8145)
                     /locus_tag="PKV56_01385"
     CDS             complement(7474..8145)
                     /locus_tag="PKV56_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOX66487.1"
                     /translation="MSQQINLFQEGLLAPKQRFSAEVMAGTVLAGAVLIVAMSLWTLS
                     TLGTLKKEAAAARAVQAQEQARLNAALAAYPSGERESAALEQQLVQAEATLQIRRALL
                     DELLRGRLDGANSRSALLQRVALTVPASAWLTDIQIEDDRLELSGRTLRPEDLRPWLA
                     RLATQPITAGQPLTDVRIERGAAPGAATAPAGAPESWGFTMVSSTGLGRATATAMSPG
                     TGAVR"
     gene            complement(8157..9191)
                     /locus_tag="PKV56_01390"
     CDS             complement(8157..9191)
                     /locus_tag="PKV56_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66488.1"
                     /translation="MISWPWKSKNASATRLAISVGDEEFHFVQAAGRQILRSGTARRG
                     RDTPQQFARRVRLIGLPDTDVVNVLGLAECQLLQIDAPAVPDNELKAAARWRIKDMVE
                     TPVDELAIDVLEVGAPRPGQRHQLYVAAAQNSRMLQVGEWMSGAGLRPTVFDIAELAQ
                     RNLQCALSEQQGRPGEVSAALVRHGAQCLLTICAQGELFYCRRLEWSVKSIDTTPSLA
                     LLAAAAVSKTRGLMSAELEGVDMIDYGAEEEDVRPAATESVSRLALEVQRSLDTWERS
                     WPDRPLDRLWVDAGSQTADFRALLERTLSLPVEPLDAWGLFEGASAADDEPIPLTLLG
                     GLLRQEARAA"
     gene            complement(9191..12157)
                     /locus_tag="PKV56_01395"
     CDS             complement(9191..12157)
                     /locus_tag="PKV56_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66489.1"
                     /translation="MKSRARIACNRLARAMTLVASMACGSAWSQCGPLGSAAEYPLYA
                     ASNLSLGSSTVSNGTSSFAVSGSGRAVNENTGVRLDPSPAPTFPALSPSSLPANGSNT
                     DSTATSQSAGTLRKISVNSTTTFAAGTYNISVLQLSNNATVKLAPGNYNIGELTMGNG
                     ASIVVSPSGVVRINIGSKFDAGSTVNLNAGGTPSNLQMFFYPNANGKASNDLKAALLV
                     YAPSSSNNLQFGDTSSITGAVIGSNIVAGKSNAFAYSPATQSSLSAVSTCGTTVALVD
                     HYELSLPTGSLACLATTVTVTACASSSSPCGTVYTGASGETATLSTSAGTLSSTTVKF
                     DNSGVATTTLTHAAAADGTPVTVTLSGESTAATNPRQCCPNGSKCSAGNSCSTTFNTA
                     GFVISSTTSGSAATIPSQTAGTASASYYLRAVQTSTTTGACTSALTGASTVNWAARCN
                     NPASCSAGNLMSIDGGKSSAIASNPSTGALSYSPVQMTFDANGNAPFTFTYADVGQVT
                     LHVTKGASGSLLSALSGASNAFVVKPAGFAVSNIKQSASPFLVNPAAAGAAGAKFVKA
                     GESFTATVTAQTSGGAATPNFGKESPAEDVLLTPTLTQPPGGKAGALANGMLAGGSFD
                     NGAATATTLAYSEVGIVTLTPGVADGNYLGAGSISGSASANVGRFVPARFALSSPAVT
                     NRSSLTCASAPDFTYLGENFQLVFTLTAQSVSGSTTANYAGSFAKLDPTSAADWNLAG
                     LGGATSFSTASGRLSLGTATGSFSNGVAKDVKLIANASRASTPDGPFPNAGFGVAPVD
                     SDGVAMAAHDMASTSGGASDHTLVGTVPLRFGRLRVSNAAGPADRALKLPVAAQYWNT
                     TFWDTNTLDSCTSVPVGAMNFGNLMRTITAADTAAIGDIVLSGGLGSLTLKAPAAGHY
                     GTYDVALSLGSKATDESCLQAWTPGVGDAATSGASLAFLRGAWCGSSLDKDPSARATF
                     GQHSTQQNHIYRRENY"
     gene            complement(12154..12681)
                     /locus_tag="PKV56_01400"
     CDS             complement(12154..12681)
                     /locus_tag="PKV56_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66490.1"
                     /translation="MSATCPERTDSLSALLRAEGAARASAAADGGFTLVSVLFIVVVL
                     AGLGAAIAHISQRQHLGSAAEIESARALQAARAGLEWAAFQVLRTPAPPAAAPACFGN
                     TSFNASGLDRYTITVSCTRTPGSGTASDGATALVFYQLQANACNAPSKGTCPSTAASP
                     DTNYVERQLTWTVQR"
     gene            complement(12662..13531)
                     /locus_tag="PKV56_01405"
     CDS             complement(12662..13531)
                     /locus_tag="PKV56_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOX66491.1"
                     /translation="MRPLPRCERRPVNATAQRGFTLVEAVLVIAITGVLFAVVGRFIV
                     QPVQGYMATVARTGLVDNADLALRRIGRDLAIALPNSVRVTASGLGIELIPTTGAARY
                     SIEGGNPLEFGVVDTSFDVVGPPLTLAAAQELVFYNLGQAVSGSNAYAENSSVGTQAA
                     SNRRRSTNAAGAATTIALDSLAGLPVGDRAPPYRVIAVSPPVSYRCDLAAGTLSRHQD
                     YGFVAAQPDPPKGGTSSVLATGVTACRFGVEGTLVAARAAIVNLSLTLSGQTSTDTES
                     VSLQHAIYVSNLP"
     gene            complement(13509..14093)
                     /locus_tag="PKV56_01410"
     CDS             complement(13509..14093)
                     /locus_tag="PKV56_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOX66492.1"
                     /translation="MRSLSLHFHAPSAHRPSRGLSLVELVIFIAVVSAALAGVLSVFM
                     QATGTSADPMLRRQALAIAESLLEEVSLMPFTYCDGDDANVATTSSIAGCAGGADAIG
                     AEGGETRFVSPQFDHVNDYNGYSMSGIVDITNTAVSGLSGYRASVAVATAALGSLTAA
                     SGDALHITVTVTDPVGNTLTLDGYRSRHAPTASL"
     gene            complement(14151..14633)
                     /locus_tag="PKV56_01415"
     CDS             complement(14151..14633)
                     /locus_tag="PKV56_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOX66493.1"
                     /translation="MTPRLRTSRLLSRGARGHRGFTLVELVMVMIIIGVLAVFALPSL
                     DLTTFRLRAFADELQAQTQAMNRLSMTQRRPVIATFTPTGATYAYASGGTLLTLACPA
                     TASPCLAEAGARSVTFNAGNSGRAITSSGAALPVTVSHGDISMAYRIENETGLFRTAP
                     "
     gene            complement(14669..15082)
                     /locus_tag="PKV56_01420"
     CDS             complement(14669..15082)
                     /locus_tag="PKV56_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOX66494.1"
                     /translation="MRSRQTGFTLIELIMVIVILGVLAAVAVPKFLDLKSDANQAAVD
                     GVAGSLSSAASVNFAARTANAKRGVAIGNCQDAAKALQTYTEGEYSTALPTQGGTYTI
                     ESQDLKPGLSANCALVFTPASGDAVKAAFTVLGIS"
     gene            complement(15227..16465)
                     /locus_tag="PKV56_01425"
     CDS             complement(15227..16465)
                     /locus_tag="PKV56_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOX66495.1"
                     /translation="MAAFAWTGRNPRGEALNGEVEAESAHAAATRLEATGVTLLSITE
                     RISAAQASPWARWSGLGSSPVSSQDLLIFSRQMYALQRAGVPILRALASLQASTAKDS
                     LIKLLIDLRASLEQGHELATAMKRHSNVFNGFYVAMIRVGEMSGRLTEVFERLARHIE
                     FELDVRARIAQALRYPTMVMVAIVIAMAVINLFVIPTFAQVFAQFKAELPPMTKLLMA
                     VSRFSVDTWPLILAAGVGGWWALRAWLATPPGRLLWDGMKLKLPIAGPIVLKGTLARF
                     ARSFAIASRSGVPISRAMTVVAETVDNAYIAQRIEQMREGIERGESLSRCATAAGVFT
                     PVVLQMIAIGEETGDIDGLLIEVADMYERETDYAIKGLAARIEPLLLAVIGALVLVLA
                     LGIFLPLWNLGQAAQGRGGG"
     gene            complement(16467..18257)
                     /locus_tag="PKV56_01430"
     CDS             complement(16467..18257)
                     /locus_tag="PKV56_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOX66496.1"
                     /translation="MAEMLTPLQWGGVVPPARTEAAPVPARAAATPTRRERLRLGDVL
                     VKQLLITETQLNEALEMQRASGKRLGRQLVDAGFVTEEAVAHGLARQLRVPAVNLKNF
                     ALNGEIVRLLPESPARRLRVIVLEDQGEQLLVGFVDPLDLAAYDEVSRLLKRQVRVAV
                     IPESQLLPALDRNYRRTDEITGLAKALELDVGSSADVGAATGISGAESAPVVRLLQSI
                     FEEAVQIGASDIHIEPQDGGLVIRCRVDGVLQTQTQADKRIAPALAQRLKLMAGLDIS
                     EKRMPQDGRFSVRVRDSGIDVRMSTMPSHHGESVVMRLLGQGKDMRRLDEIGMPRDLL
                     NQFQQILLRTSGMVLVTGPTGAGKTSTLYAALAEIDAERLKVITVEDPVEYRLPGLTQ
                     VQVNDKIELSFAKVLRATLRQDPDVILVGEIRDAETADIGLRAAITGHLVLSSLHTRN
                     AISTPFRLLDMGAPAFMVASALQAVIAQRLVRVNCTNCLEPHDPTPQEVGWLTALGGD
                     DAVGERTLRGRGCMLCNGSGYSGRIGVYEMLEMNTDLAQAATHADPVAFSRLGQEQLR
                     GRTMAHHALALVRAGTTSLAEAMRLGADSD"
     gene            complement(18425..20953)
                     /locus_tag="PKV56_01435"
     CDS             complement(18425..20953)
                     /locus_tag="PKV56_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOX66497.1"
                     /translation="MLTPLHWGGDRPPEGSAAGHPTAFCWPSPPFAAYPQVQPQTEAL
                     ACTITGLNDKLMDGQLSALHPDQGAALVQLPPSRTTLPLRFDQFRCLCLTQPLTAPSI
                     DQDDPAALLLAPGTATAFRITWKQGGELVGLTLGHVETPFGLFLFPPQDGSKTGAIQG
                     VTRMFVPAAAFSSFELGPRIGEVLVEQRSATPQQVEQAAAQQAQLRNKKVGDILVSRN
                     IVTPEQLTRALDQQARMPMVRIGEALLSMGLVSEDELQEALKQQQEDRAVPLGEILVR
                     NGIVSRADLQVAMARKMGYPLVDVDAFPVDSEALRKLPYAVATRLRVLPLLMRDNRVV
                     IAIDDPARLHSAVEEAEFSVQTKVLPVLAQSRSIDQRIHAVYEQIGAVASGRHKGDFG
                     QDTQVIEPMGTDQLVETLERENDDSITDDRPIEQSDNSLVRLINNMIIEAYKEGASDI
                     HIESYPGREKIRIRFRKDGVLRTYLELPPNYRSSLIARIKIMCDLDISERRKPQDGKI
                     NFARFSSQHRIELRVATIPTNNSLEDVVMRILVSARPIPMEQLGMSARNLEQLKAAIE
                     RPYGMVLCVGPTGSGKTTTLHSALAAINQPQRKIWTAEDPIEITQPGLRQVQVNPRID
                     WTFAKALRAFLRADPDVIMVGEIRDEETAQMAIEASLTGHLVMSTLHTNSAPETVTRL
                     LDMGMDPFNFADSLLAVLAQRLVRRLCTECRQSRPATSAEIDELLDDYLHAFELEAQE
                     QPGRDALLAQWTEQHGSDGRPVRYHSPGCKHCNHTGFKGRAGLHELMVISRDLRRMVQ
                     TGERAEALKMVAQREGMRTLRQDGIDKVLAGVTTIEEVRSTSNQ"
     gene            complement(21197..21490)
                     /locus_tag="PKV56_01440"
     CDS             complement(21197..21490)
                     /locus_tag="PKV56_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66498.1"
                     /translation="MDNDVRLAELALRRELLSLRAASQRESLRAQWQASTAPLTSLTA
                     AVDRSRSWLAPAAAAGIPLLLLLARRRRIGWRIARVALSAWSVIRLARKLMQR"
     gene            complement(21490..21867)
                     /locus_tag="PKV56_01445"
     CDS             complement(21490..21867)
                     /locus_tag="PKV56_01445"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOX66499.1"
                     /translation="MFESVRSLLALLLDIGATRLELASTELEEERLRLVGLLISAAIT
                     LMLLGMAVVMCTAFVVVAMWDSHRLLTVGGLALAYVGGTWISVNRWRARAASRPPFMS
                     VTVAELQRDIAALRASSNPPAEP"
     gene            complement(21890..22219)
                     /locus_tag="PKV56_01450"
     CDS             complement(21890..22219)
                     /locus_tag="PKV56_01450"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HOX66500.1"
                     /translation="MSQIDTAAASLQATRDELADALKQTVGEAESLLKGAGAAASHEY
                     ATLLKRAQEKLVRAKDELQRLEETAMVNARHAARVTDRAVHEHPYAAMGVAAGVGLLI
                     GMLIARR"
     gene            complement(22379..23287)
                     /gene="tolA"
                     /locus_tag="PKV56_01455"
     CDS             complement(22379..23287)
                     /gene="tolA"
                     /locus_tag="PKV56_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOX66501.1"
                     /translation="MTMAATATFSRDAFMPRRPAGLGRGLAMAVIAHLLLVAALTFSV
                     NWRVSSPEGVEAELWAAVPQIAAPRPVEVEPPPPPPVRTAKPEPEPAKPPPKEAAPVP
                     DAQIAIEKAKRLEDKRKKELAEQAREEKEAKKEAEKLARQRKAEQAEKLAEQKRKEQA
                     AEKLKTELANKKRDAAVEASNAAEREKQMQRIASLAGATGAANATGTAAQSAGPSADY
                     IGRIKGRIRPNVSFPDTLPGNPAVEVEITVAPDGRIMSSRIVKASGVPEWDDAVKRGI
                     DKTAVLPRDETGRVVSPMTIVYKPRD"
     gene            complement(23304..23732)
                     /locus_tag="PKV56_01460"
     CDS             complement(23304..23732)
                     /locus_tag="PKV56_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HOX66502.1"
                     /translation="MPAVSHRGGGRRRTINEINMVPFIDVMLVLLIIFMVSAPLITTG
                     VVDLPTVGKSKQRPEHVVELIVGRDERIRIRVDSKDEGQAVALDRIAARVLDLQAGDA
                     NTPVVISADQSVKYKTVVDVMNRLQKSGVQRVGLSVKNGG"
     gene            complement(23734..24441)
                     /gene="tolQ"
                     /locus_tag="PKV56_01465"
     CDS             complement(23734..24441)
                     /gene="tolQ"
                     /locus_tag="PKV56_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOX66503.1"
                     /translation="MNQDMSILSLVLHASVVVQLVMAGLLITSLASWTVIFSKLFGLR
                     KVRGENEEFERDFWAGKNLNDLYDEATKRSTSSAPMERIFASGMREFMKLRERRVTDS
                     GALLDGARRAMRASFQRELDTIESNLSFLASVGSVSPYVGLFGTVWGIMHAFIGLANL
                     TQVTLATVAPGIAEALVATAIGLFAAIPAVIAYNRFARDIDRLAIQMETFIEEFSNIL
                     QRNLGQPAPAPSMTGGR"
     gene            complement(24455..24886)
                     /gene="ybgC"
                     /locus_tag="PKV56_01470"
     CDS             complement(24455..24886)
                     /gene="ybgC"
                     /locus_tag="PKV56_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOX66504.1"
                     /translation="MTTVDFEFTLRVYWEDTDAGGVVFYANYLKFFERARTEWLRALG
                     HGQERMRVDGGAMFVVSDTTMRYRRPARLDDLLRITVRLAHVTRTSITLDQQAWRDDV
                     LLTEGSIRIACVEAGTFQPRRIPTEIAARLPAPTATPIPKS"
     gene            complement(24936..25316)
                     /locus_tag="PKV56_01475"
     CDS             complement(24936..25316)
                     /locus_tag="PKV56_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HOX66505.1"
                     /translation="MKSLSPFVLALLVAGCAMSPPPRPGLLDVVERPAEQALLAGLRA
                     YEDAQYTDAEKQFSLALKLGLPSPKDRAAAAKHLAFIYCTSNRLTECEASFRAAKAAD
                     PNFALGKSEAGHPLWGPVYKRVQP"
     gene            complement(25313..26839)
                     /locus_tag="PKV56_01480"
     CDS             complement(25313..26839)
                     /locus_tag="PKV56_01480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOX66506.1"
                     /translation="MSSSAPPPPDTVAGYNGPDEGGRPPAAADAAPTPEESLPSIGFI
                     GRYALKYQIGKGGLGTVYAAYDPLLSRVIAIKTLHLEIEPEQRESFNALFLNEARAAA
                     GLSHPNIVTVFDAGISEGHAYIAMALLKGQDLRQLRESGWRPSPAQAAVLVGRVADAL
                     AYAHRKGIVHLDIKPANIFMVGRGQPCVLDFGIARIAHRRDSPPGSDIAAGSPYYMSP
                     EQARQQEVDRRSDVFSLGVVLYELLTGSKPFTGVGLEQITTAVLEHTPLPAHRVNRAV
                     PKVLSDITARAMEKDVDKRFESASAMSRELRRHFAASGTGDDAAPSVWKRLGNHKAAL
                     AGALIGLVGIGALTLGAWNEQRRIGRASSAASAAASAAQQPVAQTVSASEQANAAPSS
                     QQPAASSALDASPRPAAADRPRRDKTQADARARDAAGRTGAAAAVMSKGRVRLAISPW
                     GQIEVDGAPAGTAPPLNELTLPEGRHQITIRNADFPPYSATVQVTADQPVTIKHKFGS
                     "
     gene            complement(26857..28047)
                     /locus_tag="PKV56_01485"
     CDS             complement(26857..28047)
                     /locus_tag="PKV56_01485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX66507.1"
                     /translation="MRLSKRLSHIEPFYVMECAKAASELARSPVCDASQGGRPMIFLN
                     IGEPDFTAPALVQEAAQRAIRDGNTQYTDAIGLQPLRERIARWYAQRFGLDIAPERIV
                     VTAGASAALQLACLALIDPGDEVLLPDPSYPCNRHFVAAADGVAVLVPTGPADRFQLN
                     AAAVLQAWNAKTRGVLLASPSNPTGTSIDPAEMGRIVDAVRARGGFTIVDEIYLGLSY
                     DERYGDSALRHGDDVISVNSFSKYFNMTGWRLGWLVVPESLVTSVEKLAQNLFICPSA
                     IAQHAAMACFEPDSLAEYERRRQAFRARRDFLVPALNALGLTVPVEPDGAFFAWADCS
                     AHAAHLPGGSWDWTFDLMRRAQVAMTPGRDFGRHEPSRYVRLSYANSMTQLQQAMDRL
                     KQAL"
     gene            complement(28077..28592)
                     /gene="nusB"
                     /locus_tag="PKV56_01490"
     CDS             complement(28077..28592)
                     /gene="nusB"
                     /locus_tag="PKV56_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOX66508.1"
                     /translation="MTEAEQKPAETSKPGAPAKAAKKATQKSTRRRSREFALQGLYEW
                     LLSGESPDVVSAHVREQEGFDRCDAAHFEMLLRGCIAEAAPIDAALARHVDRKTSQLS
                     PIEHGVLMIGAYELTHCIDIPYRVIINEAVELAKSYGGTDGHKYVNGVIDKTAADLRP
                     VEVDAARAARR"
     gene            complement(28589..29059)
                     /gene="ribH"
                     /locus_tag="PKV56_01495"
     CDS             complement(28589..29059)
                     /gene="ribH"
                     /locus_tag="PKV56_01495"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOX66509.1"
                     /translation="MQGADQGGPSDAELDGADLRIGIVQARFNAAITQRMAAVCIAEL
                     RRLGVDAELIDHVSVPGALEIPVALMAMADGGDFDALIAIGCIVRGETYHFELVANES
                     GAGVTRVSLDHQLPVANAILTVETEEQAWERVEDKARDAARVAIEMAHLMEDLS"
     gene            complement(29090..30199)
                     /gene="ribBA"
                     /locus_tag="PKV56_01500"
     CDS             complement(29090..30199)
                     /gene="ribBA"
                     /locus_tag="PKV56_01500"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOX66510.1"
                     /translation="MAISPIPELLTELAAGRMVILVDEEDRENEGDLVMAAEHVTAET
                     VNFMARFGRGLICLTLTRERCERLQLPPMASRNGAQHGTAFTVSIEAATGVTTGISAA
                     DRARTVQAAVARDAKASDLVQPGHIFPLQAQDGGVLMRAGHTEAGCDLSAMAGLSPAA
                     VICEIMNDDGTMARLPDLEVFAQQHGLKIGTIADLIEYRSRNETLIERTGRRKLKTAQ
                     GEFDCTSFIDRTGGLHMALTHGQWTPRDEVLVRVHEPLSVMDLLDAGACGHSWPLPRA
                     LAQLQASERGVAVLLNCGEGSDALLGKLKPAGESAVRTQSQMDLRTYGIGAQILRELG
                     VSRMKLLGSPRRMPSMVGYGLEVTGFVAADTAGRV"
     gene            complement(30237..30872)
                     /locus_tag="PKV56_01505"
     CDS             complement(30237..30872)
                     /locus_tag="PKV56_01505"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOX66511.1"
                     /translation="MFTGIITGVGRISDVKPLGDAASHGNRLHVDAPAHYLDDVGPGD
                     SIAINGACMTVTSLDAARSVFTVDVSAESLDKTAGLDSLGPVNLEKALRANDRLGGHL
                     VSGHVDGIGRVTHFAPVGESWTLKIDAPADLAKFLAYKGSITVNGVSLTVNTVADHPT
                     GCEIGINLIPHTIENTSLGHLKPGGTVNLEIDLIARYVERMQSVACVARPA"
     gene            complement(30885..32027)
                     /gene="ribD"
                     /locus_tag="PKV56_01510"
     CDS             complement(30885..32027)
                     /gene="ribD"
                     /locus_tag="PKV56_01510"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOX66512.1"
                     /translation="MTSTPSTPTDFTHRHDDWMAEALALAQQAIALSEPNPRVGCLIV
                     AADGQVIGRGHTQQAGGPHAEVMALRDAAGQGHSVRGATAVVSLEPCAHHGRTPPCCD
                     ALAEAGIARVVMALEDPNPLVAGQGAARLRATGVEVTTQGVSPQTVDAARELNIGFFS
                     RIERQRPWVRMKIAASLDGRTALPNGVSQWITGEAARTDGHAWRRRAGAVLTGVGTVV
                     EDNPRLDVRLVPTPRQPLRVVVDSRLDAPLGARLFEAPGSVLVYAAHPTTERLAAFAD
                     RGIDVARVPGVNDKVDLAAMLDDLAARGINELHVEAGHKLNGSFVRERLVDEFLIYLA
                     PKLLGAGRDLAAFGPLTDLSEAVELRFHQVERIGDDLRVLARPKLR"
     gene            complement(32046..33353)
                     /locus_tag="PKV56_01515"
     CDS             complement(32046..33353)
                     /locus_tag="PKV56_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66513.1"
                     /translation="MSTTPAFRHPRPRSQRGVTLIEALLSFLVIALGVLSMSRLHHHL
                     QAHADLARQRSQALRLAQEDIETVRAYTSLVQPAGAPPAASSYERIEPASSSLDQLGG
                     LRLNTTFQLTRQVDSGSAFRMKHATVGVAWTARDGTEHRAEVSTIIAGQDPALSGALA
                     LGPSALTASGGMARSARIPLTAKNLGNGRSVLKPVLAGSTAFVLDNLSGEVTQQCSEV
                     PAGLSNGDLTAEHLTHCSEARGLLLSGTVRFSNANPPDPLAANDPPLELAMGVALASA
                     VAAADPWCGTEAQKSVLYRSGDGIHRVAVPLAAEPARVGAAAWTDLGERFVAYNCLVP
                     AAGDPPRWSGTSTLVPFGWTIGTTAADRRVCRYAWDHDGSGAIDRNEEHPADYLGVDR
                     ALLQQNFLVIRGDLACPDGAAPRMADAVGSPPTSRVATIQHQP"
     gene            complement(33353..34648)
                     /locus_tag="PKV56_01520"
     CDS             complement(33353..34648)
                     /locus_tag="PKV56_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HOX66514.1"
                     /translation="MHPSRTETPTARRQRGLAALPVTLLLFFAMLLAAAYANRGLVFE
                     QRASANQYRAAQAFEAAEAGLEWAIAQLNHPQRIDERCLPSSTATDPSFRERFVRIDH
                     VTGQQAPVTWMSGGTPVALQPACVLGAEGWACSCPAAGHPMLDVIESDDGTPRPAYSV
                     QFDTEAGSGLIRVTSTGCTSAAGPCRPGAAGRPDASVRLQVVLGLLPGLSSPPAAPLT
                     TRGSVDAGAAALGLHHTDAASGGITVHTGGSLIGSALRLTTAPGAAAAVSASTHDAAL
                     ASMAPARLFATHFGLDKATWNGHAAVQAVACDAECGVPLAAAIDSVSSNRLISIEGDA
                     RIEGPVTLGSPDRPVVIVVDGSITLTGDVTLHGLLYASAVHWDDAGPPRGRVHGAVIS
                     ESGYGGSAAPDLIHDATLLRSLQRQTGSYARVPGSWRDF"
     gene            complement(34656..35360)
                     /locus_tag="PKV56_01525"
     CDS             complement(34656..35360)
                     /locus_tag="PKV56_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66515.1"
                     /translation="MRAPVNPSAKGQAGMSLVELLIGITLGLFITGAGITAFVVHLRE
                     SRSLIAQSRLMQDLRATTDLITRDLRRAGYWGDAAAGVWPRDPATGASGAASNPYSAM
                     AVSRLPAPAVTFRFSRDAIENGSVDDNEQFGYRLRGGTLELQLSAGRWQALTDPNAMK
                     VTRLDIAPTEQVVDLGDSCQVPCPVPSATCPPRLHVRSVTVTVAARSTTDAASERIVR
                     SSVRLRNDEVTGSCPA"
     gene            complement(35362..35838)
                     /locus_tag="PKV56_01530"
     CDS             complement(35362..35838)
                     /locus_tag="PKV56_01530"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HOX66516.1"
                     /translation="MPLPAPISTPPRRTRPRHVAGFTLIELMICISVIGVLSSVAYPA
                     FSSTIATTRRSDALIALMKVQLQQERFRADHPSYGDLAQLGLAATSPARHYRIEMLEH
                     TATGYAVHASAIGSQQRDATCRHLRLSVDGLNVVYASGATDAADNASSVNRRCWSL"
     gene            complement(35863..36444)
                     /locus_tag="PKV56_01535"
     CDS             complement(35863..36444)
                     /locus_tag="PKV56_01535"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOX66517.1"
                     /translation="MDSLQHTPRHTASQRLRRQPGFTLIEAAATLAILAVLACGAAPS
                     FHGLIERQRLAGLAAQLATDVQFTRSESILRNEALRLSFYSEAWGSCYVVHSGARNQC
                     ACTAGSSEATCIEDATQIKTVRVPTSAGASVQANVSSILFDPLHGTSTPTGTLRVRSP
                     AVGSIHHVVNVLGRVRTCSPDGPAPAIPGYRVC"
     gene            36713..37120
                     /locus_tag="PKV56_01540"
     CDS             36713..37120
                     /locus_tag="PKV56_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66518.1"
                     /translation="MDLTRVLIVDDNDMFIEMASFILGAAAYVVESASDASQALLKLP
                     VFLPDLILMDVQMPVMDGIELTRHLKADPATRHIVIVAFTAFALQGDAKKLQDAGFDG
                     YIGKPLDVMTLAAEVRFWLEGAGSVRDNRFVWP"
     gene            37185..39563
                     /locus_tag="PKV56_01545"
     CDS             37185..39563
                     /locus_tag="PKV56_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66519.1"
                     /translation="MTNGIQLPFPYSIKRHGISLSNCEDEPVQTPGCIQAHGMLLAVR
                     IDDLIVTQVSENCERWTALRVDQVLGSALSRVVGSASAERIGELVRTEALDQNPCYAL
                     TSSLPGSPAGAAVMDMTIHTADGVLLVELEPSGRVLPTCGQDGDYFSMVRKTIGRLKS
                     TRSLAEFCDVVAREARSITGMDRAMVYHFHTDDSGEVMADARREDLPSWQGLRYPAAD
                     IPKPAREIFKRIGVRPLPDAQGELCEMVPLLNPDTQRPLQMTHCALRGASVMYTEYLQ
                     NMGVAATLTMPILRDGELWGLIACHHYTAKALPYPLRSAAEFLAQIASLEIAQAELRE
                     HLQYRIDLDAVHLSVLARAAGDGQLAIMNTGSPNLLDGIHADGIAIFQEARWHTAGVV
                     PDALHLNPLAAWLRRRIDEAPDALQTFATEALGALHPPAADLAEVASGVLATVVSRHP
                     DSPLILWFRGEQLQTYNWAGNPGEKPTAIGPLGPRLTPRRSFDLWQELVRGRSTPWRR
                     VEVEAAQRLRRMVMDLVVVRADQLAVANAELASSNAELDAFAYVAGHDLKEPLRGINK
                     YAHRLLEDTKAGRIQDTERVEWLLRMTVRMDTLLNALLHFSRVGRLSLDCADIELGPV
                     LVEAMDMLGARLAESRVEVRVPRPLPMAHCDRIRVREIFANLVSNATKYNDKPHPWVE
                     IGWIGGQGEALTMQRPASTPADARGDTLFYVRDNGIGIETRNKDRIFAIFKRLHSADA
                     FGGGSGAGLTIARKMVQQHGGLIWFDSQVGEGTTFYFTLPDPASSRLGVHGA"
     gene            39553..39999
                     /locus_tag="PKV56_01550"
     CDS             39553..39999
                     /locus_tag="PKV56_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66520.1"
                     /translation="MAHDRERDGRPVLVVEDSDDDFDTVVEAATRAGVRNRLIRAAGA
                     EIARSLLAREAAGSFSFMLLDYNLPGVDGLAFLEVVRRDPVLAALPAVIYTTSVNPLD
                     RDAFLRAGANAFHLKSVQHADSLRTLESIFDHWLTRAAPAPGGPRA"
     gene            39996..41390
                     /locus_tag="PKV56_01555"
     CDS             39996..41390
                     /locus_tag="PKV56_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66521.1"
                     /translation="MNHQAQEPMRVLIVDDNPLDRADAKAALIQGSGRVYQFSEASSA
                     EEALHLCSQWPLPHCIVLDLGLPDADEFDVMIRLPRDEDNLMRIPVVVLTASVEIGLS
                     QAALRAGAQDYVGKAWLLPETLTQAVENAIERLRMTRSLEAQRRMADAARQQALQLEA
                     ENRQIQEANRLKSQFLANMSHELRTPLAAVIGFADLLLTGQVPPDSPKHRLFLGHIGT
                     SGRHLLRLVNDVLDLSKVESGKFEFFPESLDLADLVKGVNDVLATEFQLKQLRVATDI
                     EATLGGLVLDPLRLRQVLYNYLSNAIKFTPVAGLITVRAYALSARHFRLEVEDTGVGI
                     AAPDMARLFTAYQQLDAGSTKRHQGTGLGLALTRRLVMAQGGSVGVRSTPGRGSVFHL
                     VLNRIHGADAARESEYEERLDASMDARLLVMHDGHGNLNHRAGHGGLPVAVRSHFSNL
                     DGEANKRCADRKGT"
     gene            41413..42110
                     /locus_tag="PKV56_01560"
                     /pseudo
     CDS             41413..42110
                     /locus_tag="PKV56_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
     gene            complement(42209..42652)
                     /gene="nrdR"
                     /locus_tag="PKV56_01565"
     CDS             complement(42209..42652)
                     /gene="nrdR"
                     /locus_tag="PKV56_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOX66522.1"
                     /translation="MRCPYCSHVDTQVVETRESDEGDVVRRRRRCHSCDKRFTTYERA
                     EIALPAVVKKDGTRAEFDIGKVRASMSLALRKRPVSVEQIDAAIERIQEKLLNSGAKE
                     VPSTRLGELVMRELKRIDKVAYVRFASVYRSFEDVDEFRQLIRDI"
     gene            complement(42737..43987)
                     /gene="glyA"
                     /locus_tag="PKV56_01570"
     CDS             complement(42737..43987)
                     /gene="glyA"
                     /locus_tag="PKV56_01570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOX66523.1"
                     /translation="MFDRTQSTLAHIDPELWSVVQSENQRQEDHIELIASENYTSPAV
                     MQAQGSQLTNKYAEGYPGKRYYGGCEFVDLAEQLAIDRLKTLFGAGYANVQPNSGSQA
                     NQAVFFALLQPGDTIMGMSLAEGGHLTHGMPLNMSGKWFKVVSYGLNAQEEIDYDAME
                     RLAHEHKPKLIIAGASAYALRIDFERFARVAKAVGAVFMVDMAHYAGLIAAGVYPNPL
                     PHADVVTSTTHKSLRGPRGGIVLTNHEDMAKKINSAVFPGLQGGPLMHVIAGKAVAFK
                     EALAPEFKVYQQQVVTNARVLAETLISRGLRIVSGRTESHVMLVDLRPKNLTGKEAEA
                     VLGLAHMTCNKNGIPNDPQKPMVTSGIRLGTPALTTRGFKEEQTRLTAHLIADVLDQP
                     HDEANLATVRSKVAALTRDFPVYR"
     gene            complement(44113..45012)
                     /locus_tag="PKV56_01575"
     CDS             complement(44113..45012)
                     /locus_tag="PKV56_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOX66524.1"
                     /translation="MSVLRQLSLKAQQSCRATVRSTGVFMQDVGHGLLEVSHNTLALV
                     GLIAVALIVFVAGRSELRHTVEMQALGWLQARQEARTDPLELMANDLREPEAIARATA
                     SDPRDLTRQQAAVAQWLARRYKVAPEPVSRLVTEAWRVGARVGLDPTLILAIMAIESS
                     FNPFAQSPVGAQGLMQVMTRVHDDKYEQFGGNLAAFDPVTNLRVGVQVLKECIARAGG
                     VEAGLRSYVGAANLSDDGGYALKVLSEQNHLQAVAGGKLVAVNAPNVVPTASRAQAES
                     AEAPAPASESSEPPADEKLAALR"
     gene            45273..46847
                     /locus_tag="PKV56_01580"
     CDS             45273..46847
                     /locus_tag="PKV56_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOX66525.1"
                     /translation="MKYRDLREFCAGLEAVGELQRVAAPVSARLEMTALSDRVLRAGG
                     PALWFEQPLYSGGGYKIPALTNLFGTTKRVAMGMGAADIGDLREVGRVLASLKEPEPP
                     KGLKDTGRLLHLVKSLWDMKPATVRQAACQQVVIEGSDIDLLGLLPVQTCWPGDAGPL
                     ITWGLVVTRGPQGGSNPRRRQNLGIYRQQVIASRQVIMRWLAHRGGALDFRDFARANP
                     GRPFPIVVALGADPATILGAVTPVPDTLSEYQFAGLLRGSRTEVTDSGVGDAGVMLQV
                     PASAEIVLEGHIPVAASGYTGRSEHGVPLKEVGGYLYALEGPFGDHTGYYNEQDWFPV
                     FEIDRLTHRRDPVYHSTYTGKPPDEPAILGVALNEVFVPILQKQFPEIVDFYLPPEGC
                     SYRMAIISIKKSYPGHAKRLMFGVWSFLRQFMYTKFIVVTDDDVDIRNWQDVIWAITT
                     RMDPVRDVTLVENTPIDYLDFASPVSGLGGKMGLDATNKWPGETSREWGRTIAMDAAV
                     EDKVADVFDQIMRAAR"
     gene            47135..47932
                     /gene="imuA"
                     /locus_tag="PKV56_01585"
     CDS             47135..47932
                     /gene="imuA"
                     /locus_tag="PKV56_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HOX66526.1"
                     /translation="MIPESLRSVLWRGDQMERSVDPVLPSGFALLDGQLPGGGWPRRV
                     LTELLLPHPGVGEIRLLSPCLIATQRQGRLLMLFDPPAALSAQALSQLGFDVEQLLVV
                     HTRARVIPGSDSLWAFEQALKSGHVGALLAWLPPRLRADRLRRLQLAAQAHDGPAFVF
                     RELAAQERPTSAPLRLALRPGGADMLRVRVLKRRGPPLEAPLMLALPPVLSDALRAAA
                     WQRQQPAGDERIPVQEHRARKHPAGSHSASAPAAAVPLPAATFISLV"
     gene            47945..49489
                     /locus_tag="PKV56_01590"
     CDS             47945..49489
                     /locus_tag="PKV56_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOX66527.1"
                     /translation="MLWIALHLPRLSFESWLALLPPDQREQPAALMGARDVLLSNASA
                     HQLGVRPGLSRATALALAPHLRMGRADAVRDLQALTSVAHVALAFTPSVCFATTPMLA
                     AAGCVGHAGAEPGGALHTVMLQVQSSLRCFGGQQALMQRLRAAWLPLGCTVQLASAPT
                     AQGAAVLARLAEGDLGELHCADLRSMACRLDGAPVQLLGLGRDILDVLEGMGLGTLGA
                     LRRLPGRGLARRFGEGLLDALARVYGERPDPSPAWITLPDVFEVRIELQARADTTGQV
                     LHGAQLLIEPLLVWARAHQARVRRFTLFMHHEPRHRKDAGTPSVTELTIALAEPSCDA
                     DHLSVLLRERLAHLSLPAPTLELRLHCADVARGSPFSAELFASARGEREGLARLIERL
                     QARLGPQQVQRLAPAADHRTVRHGVPGRSVAQAASAPRPVWLLHEPQPLPERQSRPWL
                     DGQPLQLLCGPERVESGWWTVPRTVRDYFIAQTPEGALVWICRARLPLSVEESASGWL
                     VCGWFA"
     gene            49637..49828
                     /locus_tag="PKV56_01595"
     CDS             49637..49828
                     /locus_tag="PKV56_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66528.1"
                     /translation="MTAFKAAWYPLIAATAIVLAACQPAEGPVERAGKAVDQAAQKAG
                     DQIEKAGDKVKDAVEDAKK"
     gene            49968..50750
                     /gene="yaaA"
                     /locus_tag="PKV56_01600"
     CDS             49968..50750
                     /gene="yaaA"
                     /locus_tag="PKV56_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOX66529.1"
                     /translation="MLFLLSPAKTLDYDTPSHVDRPTTPQFVPQSAELIDILRTRSVA
                     DIATLMDLSDPLAALNVGRYGVWSTRFTKKNSKPAVLAFNGDVYEGLDAASLSADDLD
                     WAQQHLLILSGLYGLLRPLDRMQPYRLEMGTALENARGHNLYAYWGDTLARHINQRLK
                     ADPSPVIVNLASQEYFKAVQPTQKSAWLKPRVVECVFEDWKGGQYKVISFFAKRARGL
                     MARYAIQHRLLTPQGLQSFDLEGYACDAAASEPHRLVFRRRL"
     gene            50760..51632
                     /locus_tag="PKV56_01605"
     CDS             50760..51632
                     /locus_tag="PKV56_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOX66530.1"
                     /translation="MPVPQRITPELQRWIVDQATSGQSPDAVLRAMRDSGWSAAVAEQ
                     ALEDTLRDHLADRSQALALPPKLRVPEPLCSDGSLSIDLTEEASAEVRQVRVLSSLTN
                     PRIVLFGGLLSAAECDELVALAGARLARSETVESSTGGGEINESRTSDGMFFGREENP
                     LCARIERRIAELLRWPLERGEGLQVLRYRPGAEYKPHYDYFDLEQAGTPALLTRGGQR
                     VASLVMYLNTPARGGATIFPDVEFEVAPVKGNAVFFSYDRPHPVTRSLHGGAPVLEGE
                     KWVATKWLREGRFE"
     gene            complement(51653..52138)
                     /locus_tag="PKV56_01610"
     CDS             complement(51653..52138)
                     /locus_tag="PKV56_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66531.1"
                     /translation="MAIRWHTLWQGKDIAVFRDDVEVDRFAADEVERIVFVHRGRGDL
                     PTDLVFTVVELADDGLLFPVDTGFAGRVNFERQAYWTERACIYWTPHSSAPLPSSVRR
                     THWWQQLRGLTLSSGFMRVPRAELAPAMQGWPLTGPQTWEQRKWRRIERSQPFSSVDA
                     A"
     gene            complement(52269..53774)
                     /locus_tag="PKV56_01615"
     CDS             complement(52269..53774)
                     /locus_tag="PKV56_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX66532.1"
                     /translation="MSFESLGLAEPLLRAVAEYGYTTPTPIQMQAIPVVLAGGDLMGG
                     AQTGTGKTAGFTLPMLHRLMEKPAVRDAKGKLPIRALILTPTRELAAQVEESVRTYGK
                     YSKLTSMVMFGGVGMQPQIDKLRRGVDILVATPGRLLDHHQQRTLDLSHVEIFVLDEA
                     DRMLDMGFIHDIKKVLAIVPAQKQSLLFSATFSDEIKALADRLLNKPALIEVARRNQT
                     AETIAQKVHPVGREMKKDLLAHLIKSNDWDQVLVFTRMKSGANKLVEFLEKQGISAMA
                     IHGNKSQGARTKALAEFKTGDLTVLVATDIAARGIDIDMLPHVVNYELPNVPEDYVHR
                     IGRTGRAGAQGEAISLVCPDENGFLRDIERLIKREIPKEIVEGFAPPVGEKAEPIVLG
                     RMVIGEGAGRGGFRSGGGRSGGGGGGGGGGGGGRGRNDGRSAPARAGGGGAPARSGAG
                     GGGQAPRAPSRDGNAPQGNRAAPAARPAGPRPEPRREGGAMFAPRSTTPKR"
     gene            54010..54909
                     /locus_tag="PKV56_01620"
     CDS             54010..54909
                     /locus_tag="PKV56_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOX66533.1"
                     /translation="MTAPQNLCPLLWPRRAMVMQLLGSCAAWQGLAHARPASRSANPG
                     DLDPSLDAVIAGTQRSADHSARDRWRHPAETLRFFGLRADQQVLELSPGAGWYTEILA
                     PYLRERGKLYVAHYASDPSRSDTDYEPRTRKAFEAKLAATPTLYDRVVVGTLPTTAFT
                     DLHFDRPLDAVLTFRNLHNWVRDGRLLQRLRIFHAALKPGGVFGVEEHRAAPDTPLDR
                     QISSGYLTEALVIGHARSAGFELHGRSEINANPRDSRLHPHGVWSLPPNLSGAPEGSK
                     ARMEALATGESDRMTLRFIKART"
     gene            55169..56833
                     /gene="ettA"
                     /locus_tag="PKV56_01625"
     CDS             55169..56833
                     /gene="ettA"
                     /locus_tag="PKV56_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOX66534.1"
                     /translation="MAQYVFTMNRVGKIVPPKRQILKNISLSFFPGAKIGMLGLNGSG
                     KSTLLKIMAGVDKDIEGEATPMPGLKIGYLPQEPVMNPDQTVRESVEEGIGGVLAAKK
                     RLDEVYAEYAAEDADFDALAAEQAELEAIISSAGSENTDLQLELAADALRLPPWDAVI
                     GKLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLHRYAGTVVAITHD
                     RYFLDNAAEWILELDRGRGIPYQGNYSIWLDQKEKRLELEQKTEDARTRAMKRELEWV
                     RKNPKGRQAKSKARLARFEELSDVDYQKRNETNEIFIPVADRLGHEVIEFENVTKSFG
                     DRVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFRMIAGKEKPDSGNVKIGATVKMAFVD
                     QSRDSLEADKTVWQDVSGGLDNLMIGKFVVPSRAYLGRFNFKGNDQQKLVGNLSGGER
                     GRLHLAKTLIAGGNVLMLDEPSNDLDVETLRALEDALLEFAGSIMVISHDRWFLDRIA
                     THILAAEGDSKWVFFDGNYQEYEADKKKRLGEEGARPKRVRFKALK"
     gene            56920..59793
                     /locus_tag="PKV56_01630"
     CDS             56920..59793
                     /locus_tag="PKV56_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOX66535.1"
                     /translation="MTNHSPAPSLSFRAQSSSSGAISLALRRPVVPNFLAPACVLLLA
                     GCASLTPPESTPTAAAAATAAVAAPAAASMPGTSQAGVVAKLPAPGAQMPAAGSAPAS
                     APAPGAPRPFAEVIKDAKQMSGLFTLWQKDDKVWLELAPGDFNQPLFLSPKYKTGLGE
                     GRYFGGLMGEEVVIEFRRVHNQVQMLARNTEFVATPKTPNGRAVEAAFSPSLLASTTV
                     VSQPHPERKSVLVEANPLFINDMMGVAMGLQRLYRQGYGLDARNSAITDIRSGPDLVV
                     LEVLNHYATGSIAVATPGAPVPPGTPMPSVPRSVPDPRSLFMTLHYSLAKMPEQVMAP
                     RKADGRVGHFTSNLSDFSDDLARSPRQRFVNRWRLDKKDPAAALSEPVKPITFWLDST
                     IPDKYRAAVTAGILEWNKAFEKIGFKDAIRVEVQPDDATWDTLDFGRASVRWMTNSSP
                     SFGAIGPSHVDPRSGEILDADIGIESLSSRSLRSLRSQVLASSPSTDLPAELAARGMR
                     SQRACDYADQAAEQLGYALDVLEARGDIDPSSPEAEQFVLGYMKDVTMHEVGHTLGLR
                     HNFRSSRVYSEEQLTDPAFTANHSIAGSVMEYAAINLSAPGVPTSRQGTPFNSTLGPY
                     DYWAIEYAYRPLPAAQEEAELAKIAARSAEPELAFGTDEDNFLGIDPETLQFDLGSDV
                     LAFARKRIAIARDLLERQETRVLSPDQDYSILRRSVTYALRDMGRAASVLTRQIGGVR
                     TVRDHAGSGRDPLAPLPAADQRAALDLLARGFLSADSLRISPALQRKLAVDYLERGDA
                     ALRGDGTPATALVTDFSLASQVIELQRSVLGQLLSDGVAARLLDSEDKLPKDALRLSE
                     LYRRLNETVWAELGQSGDIPSLRRELQRDHINRVATQLLRPAASSRVDARSLARAQAQ
                     VLLTRLNTAARRSGLSAEAQAHLQDSIDTLQQALTAKLARTGA"
     gene            complement(59850..60689)
                     /locus_tag="PKV56_01635"
     CDS             complement(59850..60689)
                     /locus_tag="PKV56_01635"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOX66536.1"
                     /translation="MEAEATAISAAAELIADADALLITAGAGMGVDSGLPDFRGAEGF
                     WRAYPALGRRGLQFHQVASPHTFEADPALAWGFYGHRLGLYRRIAPHAGFDILRRWGE
                     GRRHGCGVFTSNVDGHFRHAGFDANALAECHGSIHHLQCSQPCSDDIWPADAFVPIVD
                     TDTCTLLNEPPRCPHCGSIARPNILMFGDSGWIEHRSSAQESSLNHWLDGASRTVVIE
                     LGAGTAIPSVRDFSQSALIDRKAVLVRINPRESAVPRRMDIALPAPALAALGAIDAAL
                     LAL"
     gene            complement(60723..61334)
                     /locus_tag="PKV56_01640"
     CDS             complement(60723..61334)
                     /locus_tag="PKV56_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HOX66537.1"
                     /translation="MRFDGKLRSWNDERGFGFIEPTQGGDEIFVHIKAFPPGTGRPVV
                     GLPLSFEVELGPQGKKRARSVEFIRTRSPGRRTRSESPAPWTPARVLAIPAFVLLYAF
                     VASQWSVKPVVAVAYLLASLLAFMAYALDKSAALQGRWRTPESTLHLFSLACGWPGAL
                     MAQQLLRHKSSKPGFRSTYWGTVALNVMGFIASHSPWLAAAIT"
     gene            complement(61571..62122)
                     /locus_tag="PKV56_01645"
     CDS             complement(61571..62122)
                     /locus_tag="PKV56_01645"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOX66538.1"
                     /translation="MWQPAGCTPAGFIADNRPGCNAGCRGQAHPPGPATQAGCHRRGA
                     SQRPRGCALLSWPVTAPCRHTGSQRPGCLLQGPAGRQGRRPRAAGPDAEDHRNREHDL
                     FDVLVQVAFAAEPVTRAERAVAAKSATAAEFTDKQQAFVDFVLAKYVRQGLDELDAEK
                     LSPLLKLKYNNAIAGAFAELGNP"
     gene            complement(62079..62780)
                     /locus_tag="PKV56_01650"
     CDS             complement(62079..62780)
                     /locus_tag="PKV56_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX66539.1"
                     /translation="MGVWWIGAQLLILRTPIAASFAPGPSLQALARLLSSGELLPHVI
                     TSLQRVAVGLGLAVAIGVPLGVLTGLSKRFAEGTTPVFQFLRMVSPLSWMPIAVMVLG
                     VGDAPVYFLLAFAAVWPMLLNTAAGVAQLDPNWLLLSRSLSATRRETVLHVVLPGITA
                     QILTGVRLAIGIIWIVLVPAEMLGVSAGLGYFILDTRDRLAYDELMAAIVLIGALGYA
                     LDVAARWMHARWLHR"
     gene            complement(62896..64146)
                     /locus_tag="PKV56_01655"
     CDS             complement(62896..64146)
                     /locus_tag="PKV56_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX66540.1"
                     /translation="MTSEAPSLASRYTHICASSDCGCGLTRRDMLRLGALATASVAAP
                     LLSAGDARAQAFKGDDQPVKIGYLPITDATPLLVAHGRKMYEAEGLRAEAPRLFRSWA
                     QIVEAFVSGQVNVIHLLTPAALWVRYGSKFPAKIVAWNHVNGSALTVAPSINSAADLG
                     GRTVAIPFWYSIHNVLLQQILRASNLVAVTRSRDGQLAPNEVNLIVMPPAEMVSALAA
                     GSIAGFIVADPFNAAAEIAGVGKVLRFSGDVWKNHACCATVLAERDLVERPEWAQRVT
                     TALVKAQLWTRENQVDTARMLSSTGEGRYTPHPLRTLTKVLAATDYASYEASGVVRHK
                     GWAQRRIDFQPYPFASYTEELVAAIRQTKVEGDTRFLEALNPKFVAGDLVDDRFVRKA
                     LAAVGGPQVFGLPADLLRKEMVQV"
     gene            complement(64173..64973)
                     /locus_tag="PKV56_01660"
     CDS             complement(64173..64973)
                     /locus_tag="PKV56_01660"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66541.1"
                     /translation="MNAEPADSSAAPYALSGRGLAFSYGKGQPIFESVSLDVRPREIV
                     ALLGGSGCGKSSLLRVLAKLAEPSRGEIEFLGAPLLAPHPRAALVFQQPSLLPWLSVA
                     GNVGFGLDFSHQPQIDRVALDKRVRDALEAVSLSRHDKLYPAQLSGGMAQRVSLARAL
                     AREPQLLLADEPFSALDAITRAEMQTLLVELVHRWHTAALLVTHDIDEAILVADRILL
                     MGRAEPAQPARVLHEWTVSIERPRESHAAEVTALRLDILAALQSLRHA"
     gene            65379..>65925
                     /locus_tag="PKV56_01665"
     CDS             65379..>65925
                     /locus_tag="PKV56_01665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX66542.1"
                     /translation="MSSTETTTTTVKTYLRPIDRDGLLAFAEKGRNNPASRGTNKVHT
                     VVEGQYRTLSYVGNHAPVVVDEPPHLFGQDTAPAPGEIVLSALGGCLAVGITAVATWK
                     QVRLSKLELFLEGDIGNPAAWGAGGALKAPADMGFQAIRVKVVVEGDATREVLDEIVK
                     HANMYSPVANSMRNPIPFQIDL"
BASE COUNT        10225 a        21902 c        22753 g        11045 t
ORIGIN      
        1 tggcccagca gggcctcacc gagaaggatg tcaccctggt caacatggcc gcgcccgacg
       61 ctggtgcggc gttcgccgcc aagtcgatca acgccgcggt cacctgggag ccgttcggta
      121 cgcaaggcat caagtcgggc ggcaagctgc tgctgaccag caaggacacc cccggggcca
      181 tcgtcgacgt gctgggcgtc ggcaacgacg tgctcaagaa tcgccccgac gccgtgaagc
      241 tgctggtggc gagctggttc gatgcgctcg agtacgtcaa gaagcaccag gccgagtcgt
      301 tcaagatcat ggccaaggcc tcgggcgtat cggtggccga gttcaccgag atgtggaagg
      361 gcgtgcgcat ccccacgctg gccgagaacc agaccgcgct cggcaccgcg cccggctcgg
      421 gcagctaccg caagacggtc gaatcgatga gcagcttcat gatcgagcag aagctcatca
      481 cgcagccggt ggcggccgac agcatggtct ccggcgcctt cctgccggcc aagtgagccc
      541 cgggccgcgg ctagcgcgtg gcctgccgcc ggtagcgcag tggcggcacg ccgacccggc
      601 gggtgaaggt ggtggtcagg tgtgactggt cgaagaagcc gcagcgcaag gcgatctcgg
      661 caatgaccag gcgggagttc accagaagat cccgggcctg atccacgcgc aggccgatca
      721 ggtactgctg cggggaccgg cccgtcgcgg ccttgaaagc cgccccgaac cgttgcggcc
      781 ccatgcccgc gatggcagcc agatcggcga cttccagcgg ctcgttcagg tgctcgatac
      841 agcagtcgtg aatgcgcttg agcatcgccg cgggcaaggc tgcgggcggg cgtcgccttg
      901 gggagtcgcc cgtggcgctc gccactcgca acaggccgca gatcagcgct tcttcggctg
      961 aacgggcgaa gcgccggccc agcgcgtcgg tcgctcgcgt ctcgacccag gcccgtcggc
     1021 acagatcgct caggaacggg tccttgaacg agcgtgcctg cagcggctcg agctgctcca
     1081 ccgcctggcc ggtttcggcc gcgatgcggg ccacggcatc ggcgccgatc gagatcatca
     1141 ggtgacgttg tgcctgcgag aggtgcagcg tggccccggc atggggcggc gtgatcggcg
     1201 cgaacatgcc cggacgcaac cagtcggtgt gccagcggtc gtcgatacgg tggctcagcc
     1261 gggcctcgcc ctgcagcacc aggcacaggg tgaagtgggg ggcgggctcg tcgtgcagtt
     1321 cggtcgcgtc caggttcacg tcatcgaagc agagcgccgg gcgaacgggc gagggatcga
     1381 gtgctgcggc ggagttcatg ccgcggtgac ttgcagttcc cgtgccgcgg cggccagatc
     1441 aacgcgctga tgaccaaaag ggctgcgctg gttgccgctg gcggtgtctc ttcggttcga
     1501 agcggtcgat gccaaccagc gctcgcagta caggcggcat ttcgatctgc atgggcaggg
     1561 cggggacggg tgatcacgtg cgccgtccct gggttttcct tgggggcatc cgtttcggtg
     1621 accatcggcg atcgtggttg aattcgggtc cggctcggcc gcagcggcgg cccgtctcca
     1681 tcgaaaggaa tgcagtgaac aagctctgga tcggattgat gggggctgcg ctgagcgcca
     1741 gcgccgcggc cgaatggacc gagatcggcg gcaacgagag catcacgttc tatgccgatg
     1801 tcgcgacgct cgattcgtcg ggcggcacca agaagatgtg gacgctcgtc ggctcgaagt
     1861 ccgagcgcaa gtacggcgag atcaagttct cgtcgatcaa gacgcagttc gagttcgact
     1921 gcccggcgca gaaggtccgc cagctcgaga cggcgttcta tgcgggtccg ctggccgatg
     1981 gcgagcgcgt ggcgggttac gccgacaccg gcgattggga gccgatcgtc gagggcacgg
     2041 tcaaggacgg cctggcgaag atcgcctgcg aaactgccgt cccgaaatcc tgacgcagcc
     2101 cgtccgaccg gcgcgccggc ctcaatccct gctgtggcgc tcgatcaaat cggcaatgcg
     2161 ctgtgccgcg tcgccttcct gcgccatgag cgccgagtca cgccggcacg cggcggcgat
     2221 cgccgggtcg ttgatcaggc ggtcgatcgc ctgtgaccag cggcgagccg agccgcgcgc
     2281 cggcagccgc acgccgaccc cgcgcttgac gagtcgagtc gcgttgtcga actggtcgtg
     2341 tgcatggggc accagccctt gcggcacgcc cgctgccagc gcctgagccg tggtgccgat
     2401 gccgccgtgg tggaccagtg ctgccaggcg cggcagcagc aggtcgaagg gcgcgtagcc
     2461 caccgcttgg gcgaacggcg gcagcggctg gggcagttga tccgcgtacg gcgtgatcag
     2521 cagtgcgcgc cggttcagtg ccgcgcacgc tgccagggcg cgcgcgaaga agtcccggcc
     2581 gtgcgccatc gccgaacctg gcgtgaagcc gatcggcgcc gtgccgccct ccaggaactc
     2641 gaccagcgtc ggttccagcg aggcgtccgc cgcagaccag cgcggaaagc cggtggtcac
     2701 tgcttgtgtc ggccagtcgg cctgatgcgg tgcaaaccac tccggccagg cgcacaccac
     2761 ttgctgcggt gaattcaccc agtggctcat gaccctgcga acggggggca gcccgagagc
     2821 gcgccggatc ggatcgaagc cggggacgat cacgcggtcg atgaagccgc gttcgacgct
     2881 gctgtggatc aggcgaacca gcggtgctgg ccagccgctc agatcgccca cgccgggcag
     2941 caccgccggt gcctgggccg agaacatgca gatcggcgac aggtgcaccg tgacggccgg
     3001 caggccgtgg gtctcctgcg ccagccgaac cggccaggcc agcgtgctgc cgaccagcat
     3061 cgtgcggccc ggctgcacgc gcgacagcag gctgcgatgg gcctcgagca gccggggctg
     3121 catttcctgc cacagcaccg ggaagctgcg tcttggatgc cacaacgcgg cctgctgcac
     3181 gacacgctgg taatcggcct cggtaccgac cggcgtgaag cccaggccgg cggcttcgat
     3241 ccgcgtccgg aaatgcggcg aagtgagcag ttcgacgtca tgcccgcgct gcgccagggc
     3301 cgtgccgatc gcgatgaagg ggtgcacgtc gccggcgctt ccgacacagg aaatgaggat
     3361 gtgcatcacc gattgtgtgc agcacgacaa tggcgcccgg cttcccggtt caaccccccc
     3421 atgcgcatcg accagatccg acagcggctg cgcgcccacg gtgcgctgcc ttgtcacgag
     3481 caccgcgtgc tgcggctgtg gtccaacgcg ttgtcgcagg acagcgggcg tcgcaagccg
     3541 caggacttcc tgccgctggg cgtgcgcgcc gaactggctg cactcgaaac cgatctgcag
     3601 ggactggcac gcctgatttc cgaacacccg gcagacgatg gctcggcgcg cctgctggtc
     3661 gggctgcacg acgggcagac ggtcgagtcg gtgctgctgc cgcgcgatgg gctgtgcgtg
     3721 tccacgcagg tcggctgcgc ggtcggctgc gtcttctgca tgacggggcg ggacggcctg
     3781 ctgcgccagc tgggcagtgc cgagatcgtt gcgcaggtgg cgctcgcgcg cagcctgcgt
     3841 cccgtgaaga aggtcgtcct catgggcatg ggcgagccgg cgcacaacct cgacaacgtg
     3901 atggacgcga tcgagctgct gggcaccgag ggcggcatcg gccacaagaa cctggtgttc
     3961 tcgacggtcg gcgatcggcg cgccttcgag aggctggggc agggcccggt gaagccggcg
     4021 cttgcgctgt cgctgcacac cacgaaggcc gcgctgcgcg cgcggttgct gcctcgtgcg
     4081 ccagccatcg agccggacga actggtcgca ctcgctgaag gttatgcgca ggccaccggt
     4141 tacccggtgc aggtgcagtg gaccttgctg gcaggcatca acgacggaga cgacgagctc
     4201 gacggcatcg tgcggctgct cgccggcaag cgcgcgatcc tgaacatgat tccgtacaac
     4261 gacgtcgacg gtctcgactt ccagcggccg cttgccgagc gcgcgcggga gatcgcagtc
     4321 acgctgcacc gccgcggcgt gctgaccaag ctgcgccagt cggccggtca ggacatcgat
     4381 ggcggctgcg gccagctgcg agcgcggcag gcgcccgatc ctgcgcacgt gcccgacgac
     4441 cggcttcgca tcgtcgctgt tcgtccgcgc tgacgcgctt cagcgtgcct cggcctgcgc
     4501 cgcaggtctg atccccgctg cagcgaccgt cgctgccggc gcgccttccg tggcagtcgg
     4561 tgcaggcttc accgagatca cgcggcgcgg cgtatcgggc gactcgtcga agaggctgcc
     4621 ggtctgctgg ttggcctgct gccagtcctc gacgttgcgg atgatcgtgg gcttgatcag
     4681 caccacgagt tccttcttgc ggctgctcac gtcgttgttg ctgaagagcg cacgcgtgac
     4741 cacgttgttc gagctgcccg gcagccccga gttgcggccg ttggcctcga cctgcatcag
     4801 cccgccgatg gccacgatgt tgccgtcggt cacccgaacc accgtgtccg tctcgttcac
     4861 gctgctcgat gccagcggca gcttgtagct gccgatcgtt ccgaggtcga tctgcttggt
     4921 cttctcggtc accgccgtca ccgacgggtg cacgtgcagg gtgatggtgt tggcttcgtc
     4981 gatctgcggc gtcacgtcga gcgcgatgcc cgagaagaac ggcgtcagcg tcagcgtcgg
     5041 caacgtggtc gtgccgttgt tgttgttgtt tcccgcggtg atggttccac ccgacacgtt
     5101 ggtcacgaag tactcgtcgg tgccgacctt gagcacggcc ttctggttgt tcagggtggc
     5161 gacccgcggg ctcgacagga tctgcacgtc accgtggctt tcgaggaacc ccagcaccgc
     5221 ctggaagcca ttggtcgcca gcgccaggcc cagcgtgcca gccccggctg tcggcagcga
     5281 aacgtggtcg atcagcagcg aactcacggc cgacggcagc gtggggatgc ccttcgagtt
     5341 ggacaccagc gcgttgccgg cgttgccgct ggtctggccg atgctgccgc ggctgcgcag
     5401 gatcgaccag tcgatgccgc tctggaatcc ctcgcgcagt tccacttcca cgatcttggc
     5461 ctcgagcatc acctgccgct cgatggcgag gcgcgaggct ctcaggtagg cctcgacctg
     5521 tcgaagctct tcgggcatgg cgcgtaccgc gatcgtgccg gactgcggac tggtgatcac
     5581 gttgcggccg ttggcgttgc cgacgatgct gcgcagtgcc tcgctggtct ctgcccaaac
     5641 gtcggaactc gaggtggtcg agatctggct gctgtcgagc gacgcgccgc cattgccctg
     5701 gttgctgccg ttcggcgatc cgctgctgtt gccggagttg ttgtagttgt tgttgccgtt
     5761 gttcgtcacc ggcgccgatc cggagctgac gcggacgtcg ctgcgcccct ggcgcttggc
     5821 ctgcaggtag ttggccatga agatgcgcgt ctgcagtgtc ggcgggtaca cggtgatgcg
     5881 gcgtccgtcg atgcggaagt cgtagccgta gacgtcgcgg atcgactcga gcgcctcacg
     5941 caccgtcacg ccgcgcagca tcaccgacag cgtgccgccg acatcggggt gcatcagcat
     6001 gctgtagcgg gagtcgctga ccatcgccag gaagacgtcg cgggctgcgg caccgctcac
     6061 gctcaggtcg aagcggggtt ccggcggcgt ttcgggccgg ggagccggcg cggccgcaag
     6121 tggcgacggg ctggctgccg cgggcgcctc gactgtggct gccgacgcag ggctcgctgc
     6181 gcgcgccggt gtgccggacc gggcggcgag cagttcatcg cgcacggtgt cgccggcgtt
     6241 cgggctgcgc agcggggtct gcagtgcgca tccgggcagc agggcggcga tgcccagagc
     6301 cagcagcgtc gcggccccgc gatgccgggg ggcgctgtga ctcgcttgaa tgggtttcat
     6361 catggcgact cgcctcgtat caaagtttgg gaacagcggt gtttcgggtt cttgcaggcc
     6421 ttgcgcgcgg gccggctgtc agatgcggcg gcggctgact ccaccgcgac ctgcttgacg
     6481 acattgccgt ggagcgggat ccgggtgacg ctgccaccct cgctcaccca cacgtccgtt
     6541 tcggtgattc gttcgatgcg tgcgctgccc agcaggtcgc ccacgccgcg gcgccgcgtg
     6601 ccctcgacca catagggcac gccgtcgatg cgcaccaggt gcttcggcgt ggacggcggt
     6661 gcggcgaccc caccgggtgc gttcggggtg gccgcagtcg gacgcgctgc cgcaggcggc
     6721 accgtcggat cgcgcatcgt ctgtgcgtcg atgccctggg cgtgcaccgc gagcgacatg
     6781 gccgctgcgg ccagcgccag ccaggcctgc ctgcttgcag cgaggcgctt catttcacct
     6841 ctccggccag gaacaggcga acgctgagtt cgggcggcgt ctgctcggca tggatcttca
     6901 gcggacccca gcgcagttca ggcagggcgc gctcgagatc ggcgaggtac tgcacgatgt
     6961 cgaggtagct tccgctgaca cccagatcga cggcttgcca ctgcagcgaa gccgccgcgg
     7021 gcgcatcctt gcccttcgcg gcggacttct cgccgggccg ttctgcgacg gtgatcagcc
     7081 gggtcagcgt cagtcgatcg ttgcggcgca gcacctgatc gaccagttcg ggcagccggg
     7141 ccggccgtcc cgcgtcgccg aggcgttctc cgatctggcg atccacgttc ttcgtctcgt
     7201 cgatagagcg cgcaagccgc tgctgcaggc tgtcccgggg cccgtccgaa cccgcgtcgc
     7261 gggcggccga gttcagcttc tggcgcaggg cgtcgagctc gcgggcctgt gttgcggctt
     7321 cggcgcgcag cgcgttctgc tgcgccttgg caggcgccag caccagcgtg tcggctatcg
     7381 cccccatcac gaacatgacc gatacgacga tcatcagccg ctcacgcagg ctcagcgcct
     7441 cgatgcgccg ccagtgctgc ttccacagag gcttcatcgc accgctcctg tgccggggga
     7501 catcgccgtt gcagtggcgc ggcccagtcc cgtgctgctg accatcgtga agccccagct
     7561 ttcgggtgcg ccggccggtg cggttgcggc accgggcgct gcgccacgct ctatcctgac
     7621 gtccgtgagc ggctggcccg ccgtgatcgg ctgggtcgcc aatcgcgcca gccacggccg
     7681 caagtcttcg ggacgcaggg tgcgcccgct cagttccagc cggtcgtctt cgatctggat
     7741 gtcggtcagc caggccgaag ccggcaccgt cagcgcgacg cgctggagca gcgccgagcg
     7801 gctgttcgcg ccatccagcc ttccgcgcag cagttcatcg agcagtgccc gacggatctg
     7861 cagcgtcgct tcggcctgga ccagttgctg ctcgagtgct gcggactcgc gttcgccgga
     7921 tggatacgcc gccagcgcgg cgttcagccg ggcctgctcc tgggcctgga cggcgcgtgc
     7981 tgcggcggct tccttcttca gcgtgcccag cgtggacagg gtccacaggc tcatcgcgac
     8041 gatgagcacg gcgccggcca ggacggtacc ggccatcact tcggcagaga agcgctgctt
     8101 cggtgccagc agcccttcct ggaacaggtt gatctgttgc gacatggtcg atgtcctcag
     8161 gcggctcgcg cttcctggcg cagcagaccg ccgagcagcg tcagcgggat cggttcgtca
     8221 tcggccgcgg acgctccttc gaacaggccc caggcatcga gcggctcgac cggcagcgac
     8281 agcgtgcgct ccagcagggc gcggaaatcg gcagtctggc tgccggcatc gacccacagg
     8341 cgatcgagcg gccggtcggg ccaggatcgt tcccaggtgt ccagcgagcg ctgcacctcg
     8401 agcgccagcc gcgagacgga ttcggtggcg gcgggccgca cgtcttcctc ttccgccccg
     8461 tagtcgatca tgtcgacgcc ctccagctcg gccgacatca ggccgcgtgt cttcgagacc
     8521 gcggccgcag ccagtaaggc caggctcggc gtggtgtcga tggacttgac cgaccattcc
     8581 aggcggcggc aatagaacag ctctccctgc gcgcagatgg tcagcaggca ttgcgcgccg
     8641 tgtctgacca gcgccgcgct gacttcgccg ggtcggccct gctgttcgga caaggcgcac
     8701 tgcaggttgc gctgggccag ttcggcgatg tcgaagaccg tcggccgcag acccgcaccg
     8761 gacatccatt cgccgacctg cagcatgcgg ctgttctgcg ctgccgccac gtagagctga
     8821 tgccgctggc ccggccgtgg cgctccgacc tcgagcacgt cgatggccag ttcatccacg
     8881 ggcgtctcga ccatgtcctt gatgcgccat cgggccgcgg ctttcagctc gttgtccggg
     8941 acggcgggcg cgtcgatctg cagcagctgg cattcggcca gaccgagcac gttgacgaca
     9001 tcggtgtctg gcagcccgat caggcgcacg cgacgcgcga attgctgtgg cgtgtcgcgg
     9061 ccgcgtcggg cggtgccgct gcgcaggatc tggcggcctg cagcctgcac gaagtggaac
     9121 tcctcgtcgc cgaccgagat ggcgagccgc gttgccgatg cgttcttcga cttccagggc
     9181 caactgatca tcagtagttc tcccgccggt agatgtggtt ctgctgcgtg ctgtgctgcc
     9241 cgaacgtcgc gcgcgccgag gggtccttgt ccaggctgct gccgcaccat gcgccgcgca
     9301 ggaacgccag cgaggcaccg gatgtggccg catcgccgac gccgggtgtc caggcctgca
     9361 ggcaggattc gtcggtggcc ttgcttccca ggctgagcgc cacgtcatag gtgccgtaat
     9421 ggcccgctgc aggcgccttc agcgtcagcg agccgagccc gccggacagg acgatgtcgc
     9481 cgatggcggc ggtgtcggcc gcggtgatcg tgcgcatcag gttgccgaaa ttcatcgcgc
     9541 cgaccggaac actggtgcag ctgtcgagcg tgttggtgtc ccagaaggtc gtgttccagt
     9601 actgcgctgc aacgggcagc ttcagggccc ggtcggcagg ccccgccgcg ttcgaaaccc
     9661 ggagccggcc gaagcgcagc ggcaccgtgc cgaccagcgt gtggtcgctg gcgccgccgg
     9721 aagtgctcgc catgtcgtgc gcggccatcg ccaccccgtc gctgtcgacc ggagccacgc
     9781 cgaaaccggc gttcgggaac gggccgtccg gggtgctggc gcgcgatgcg ttggcgatca
     9841 gcttcacgtc cttggccacc ccgttgctga agctgccggt ggccgtaccc agcgacagtc
     9901 ggccgctggc ggtcgagaag ctggtcgcgc cgccgagccc ggcgaggttc cagtcggcgg
     9961 ccgaggtcgg atcgagcttg gcgaagctgc cggcgtagtt ggccgtggtg ctgcccgaga
    10021 cgctttgcgc ggtcagggtg aagaccagct ggaagttctc gccgaggtag gtgaagtcgg
    10081 gggcgctcgc gcacgtgagc gagctgcggt tcgtcacggc cgggctcgac agcgcgaatc
    10141 gggccggcac gaacctgccg acgttggcgc tggcgctgcc gctgatgctg ccggccccga
    10201 ggtagttgcc gtccgcaacg cccggcgtca gagtcacgat gccgacctcg ctgtacgcca
    10261 gcgtcgttgc agttgcagcg ccgttgtcga agctgccgcc agccagcatg ccgttggcca
    10321 gcgcgccggc cttgccgcct ggaggctgcg tcaacgtcgg ggtcagcagg acgtcttcgg
    10381 cgggcgactc cttgccgaaa ttcggtgtgg ctgcgccgcc cgaggtctgc gcggtcacgg
    10441 tggccgtgaa actctcgccg gccttcacga acttggcacc cgccgcgccg gccgcggctg
    10501 ggttgacgag gaacgggctc gcggactgct tgatgttgct gaccgcgaag ccggcgggtt
    10561 tgacgacgaa ggcgttcgac gcgccgctga gcgcgctcag cagcgatccg ctcgcgccct
    10621 tggtgacgtg cagcgtcacc tgaccgacgt ctgcataggt gaacgtgaac ggcgcattgc
    10681 cgttcgcatc gaaggtcatc tgcaccgggc tgtagctcaa tgccccggtg ctcgggttgc
    10741 tcgcgatggc ggaagacttg cctccatcga tgctcatcag gttgcctgcg gaacagctgg
    10801 ccgggttgtt gcaccgggcg gcccagttga ccgtgcttgc tccagtcagc gcggaggtgc
    10861 acgccccggt cgtcgtgctg gtctgcacgg cacgcaggta atagctggcg gacgcggtcc
    10921 ccgctgtctg cgaggggatc gtggcggcgc tgccggaggt ggtgctcgag atcacgaagc
    10981 ctgcggtgtt gaaggtcgtg ctgcagctgt tgccggccga gcacttgctg ccgttgggac
    11041 agcactggcg aggattggtc gccgccgtgc tctcgcccga cagcgtcacc gtcaccggcg
    11101 tcccgtcggc cgccgccgcg tgggtcaacg tggtggtggc gacgccgctg ttgtcgaact
    11161 tgaccgtcgt gctcgacagc gtccctgcgc tggtcgacag cgtggccgtt tcgccgctgg
    11221 ctccggtgta gacggtgccg cacggggacg agctgctggc gcaggcggtc acggtcaccg
    11281 tggtcgccag gcaggccagg ctgccggtcg gcagcgacag ttcgtaatgg tcgaccaggg
    11341 cgacggtcgt gccgcaggtg ctgaccgccg acaggctcga ttgcgttgcc ggcgaatagg
    11401 cgaaggcgtt cgacttgcct gcgacgatgt tgcttccgat caccgcgccg gtgatcgagc
    11461 tcgtgtcgcc gaactggagg ttgttcgagc tcgagggtgc gtacaccagc agggcggcct
    11521 tcaggtcgtt actcgccttc ccgttggcgt tcgggtagaa gaacatctgc aggttcgacg
    11581 gcgtgccgcc ggcgttcagg ttgacggtgc tgcccgcatc gaacttgctg ccgatgttga
    11641 tgcgcaccac gccgctcggg ctgacgacga tggacgcgcc gttgcccatg gtgagctcgc
    11701 cgatgttgta gttgcccggc gccagcttga ccgtcgcgtt gttcgacaac tgcagcaccg
    11761 agatgttgta ggtgccggct gcgaaggtcg tcgtgctgtt caccgatatc ttgcgcagcg
    11821 tgcccgccga ctggctggtg gcggtgctgt cggtgttgct gccgttggca ggaagcgagc
    11881 ttggcgacag cgccgggaag gtcggtgccg ggctcgggtc caggcgcacc cctgtgttct
    11941 cgttcaccgc gcggccgctg ccgctgaccg cgaaactgct ggtgccgttg ctcacggtgc
    12001 tgctgcccag cgacaggttg gacgcggcgt acagcgggta ctcggctgcc gatcccagcg
    12061 gcccgcactg cgaccaggca ctgccgcagg ccatgcttgc gacgagggtc atcgcccggg
    12121 ccagccggtt gcaggcgatg cgcgcacgtg acttcatcgc tgcaccgtcc aggtcagctg
    12181 ccgctcgaca tagttggtgt cggggctggc cgcggtgctc gggcaggttc ccttgctcgg
    12241 cgcattgcag gcgttggcct ggagctgata gaagaccagc gccgtagcgc cgtcgctggc
    12301 cgtgccgctg cccggcgttc gggtgcagct cacggtgatc gtgtagcgat cgaggcctga
    12361 ggcattgaag ctggtgttgc cgaagcaggc cggagcggcg gcgggtggtg caggggtgcg
    12421 cagcacctgg aaggccgccc attccagccc ggcgcgtgct gcctgcagcg ctcgtgccga
    12481 ttcgatctcg gcggccgagc cgagatgctg tcgctggctg atgtgggcga tggccgcgcc
    12541 caggccggcc agcacgacga cgatgaacag caccgagacc agcgtgaagc cgccatcagc
    12601 ggcagcgctg gcgcgtgcgg ctccttcagc ccggagcagc gcgctcaggg agtcggtgcg
    12661 ctcagggcag gttgctgaca tagatcgcat gttgcaggct cacgctttcg gtgtcggtcg
    12721 aggtctggcc ggacagcgtc aggctgaggt tgacgatcgc ggcccgggcc gcgaccagcg
    12781 tgccttcgac gccgaaacgg caggcggtca cacccgtggc cagcaccgag ctggttccgc
    12841 ctttcggtgg gtccggctgt gcagcgacga agccgtagtc ctggtgacgc gacagcgtgc
    12901 cagcggccag gtcgcaccgg tagctcacgg gtgggctcac cgcgatgaca cggtagggcg
    12961 gcgcgcgatc acccaccggc aggccggcca gcgagtccag cgcgatggtg gtggctgcgc
    13021 cggcagcgtt ggtgctgcgg cgccggttcg aggcggcctg cgtgccgacg ctgctgttct
    13081 cggcataggc gttggagcca ctgactgcct gcccgaggtt gtagaagacc agctcctggg
    13141 cagcggcgag ggtcagtggc gggccgacca cgtcgaagct ggtgtcgacg acgccgaact
    13201 cgagcgggtt gccgccttcg atgctgtagc gggccgctcc ggtggtcggg atcagttcga
    13261 tgcccagccc cgaagcggtc acccgcacgc tgttgggcag tgcgatcgcg aggtcgcggc
    13321 cgatgcgccg cagggccaga tcggcgttgt cgaccagtcc ggttcgggcc acggtcgcca
    13381 tgtaaccctg caccggctgc acgatgaatc gaccgaccac cgcgaacagg accccggtga
    13441 tggcgatcac cagcaccgcc tcgaccagcg tgaagccgcg ctgcgccgtt gcgttgaccg
    13501 gacggcgctc acagcgaggc agtgggcgca tggcgggatc ggtagccgtc gagcgtcagc
    13561 gtgttgccga ccggatccgt cactgtcacg gtgatgtgca gcgcatctcc gctggcggcg
    13621 gtcaggctgc ccagcgcggc ggttgccacg gcgacgctgg cgcggtagcc cgacagcccg
    13681 ctgactgcgg tgttggtgat gtcgacgatg ccgctcatcg agtagccgtt gtagtcgttg
    13741 acgtggtcga actggggcga gacaaagcgc gtttcgccgc cctccgcacc gatcgcgtcg
    13801 gccccacctg cgcagcctgc gatgctgctg gtggtcgcca cgttggcgtc gtcgccgtcg
    13861 cagtaggtga acggcatcag gctgacttcc tccagcagcg actcggcgat ggccagcgcc
    13921 tgccgccgca gcatcggatc ggcgctggtg ccggtcgcct gcatgaacac gctcagcacc
    13981 ccggccagcg ccgcactgac cacggcgatg aagatcacga gttcgaccag cgacagaccg
    14041 cgcgatgggc ggtgcgctga aggtgcgtgg aaatgaaggg agagcgatcg catcggcgcc
    14101 tcggagggtt ccgaagtcga tgctaggacg cgagcttccg gttcaatgcc tcaaggagcg
    14161 gtgcgaaaga gccctgtttc attctcgatc cggtaggcca tcgagatgtc gccgtggctg
    14221 acggtgaccg gcagcgctgc gccgctcgag gtgatggcgc gaccgctgtt gccggcgttg
    14281 aaggtgacgc tgcgggcccc ggcttcggcc aggcagggcg atgcggtggc cgggcaggcg
    14341 agcgtcagca gtgtgccgcc ggacgcgtac gcgtaggtgg cgccggtggg ggtgaacgtg
    14401 gcgatcacgg gccggcgctg cgtcatcgac agccggttca tggcctgggt ctgcgcctgc
    14461 agttcgtcgg caaacgctcg cagccggaac gtggtcaggt cgagcgaggg cagcgcaaag
    14521 acagccagca cgccgatgat gatcatcacc atcaccagtt ccaccagcgt gaagccacga
    14581 tggccccgcg ccccgcgcga cagcagtcgg gaggttcgga gccgtggagt catgccgagt
    14641 cggggcgacc tgcgccccgc gtgccgtgtc agctgatgcc gagcaccgtg aacgccgcct
    14701 tgactgcatc gccggaggcc ggcgtgaaga ccagggcgca gttggccgag aggccgggct
    14761 tcaggtcctg cgattcgatc gtgtaggtcc cgccctgggt cggcagcgcg gtggagtatt
    14821 cgccttccgt gtaggtctgc agcgccttgg cggcatcctg gcagttccct atggccacgc
    14881 cacgcttggc gttcgccgtg cgggccgcga agttgaccga tgcggccgag ctcagcgagc
    14941 cggccacgcc gtccaccgcc gcctggttgg catcggactt gagatcgagg aatttcggaa
    15001 cggccaccgc ggcgagcacg ccgaggatga cgatgaccat gatcagctcg atcagggtga
    15061 agccggtctg tctggagcgc atctcggaat ctcctgttgg aaaatggatt cgcctgacgc
    15121 acgcatcacg catcgcatcg aggcctgcgt gctctatcgg ttgttacccg catgacttga
    15181 ggccgaagtc ggagtccgcg gacggattgc gcagtccttc tcgaagtcag ccgccgccgc
    15241 gtccctgagc ggcctgcccg aggttccaca gtggcaggaa gatgcccagg gccagcacca
    15301 ggacgagggc gccgatcacg gccagcagca gcggctcgat ccgtgccgcc aggcccttga
    15361 tggcgtagtc ggtttcccgc tcgtacatgt cggcgacctc gatgagcagg ccgtcgatgt
    15421 cgcccgtctc ctcgccgatc gcgatcatct gcagcaccac cggcgtgaac acgcctgccg
    15481 ccgtcgcgca gcgcgacagg ctttcgccgc gctcgatgcc ctcgcgcatc tgttcgatgc
    15541 gctgcgcgat gtaggcgttg tcgaccgtct cggccaccac cgtcatcgcg cggctgatcg
    15601 gcaccccgct gcggctggcg atcgcgaagc tgcgcgcaaa cctcgccagc gtgcccttga
    15661 gcacgatggg gccggcgatc ggcagcttca gcttcatccc gtcccacagc aggcgaccgg
    15721 gcggggtggc gagccacgct cgcagggccc accatccacc gacaccggcg gcgaggatca
    15781 gcggccaggt gtcgacgctg aatctcgaca cggccatcag cagcttcgtc atcggcggca
    15841 gctcggcctt gaactgggcg aacacctgag caaaggtcgg gatcacgaac aggttgatca
    15901 cggccatggc gatcacgatg gcgaccatca ccatcgtcgg gtagcgcagc gcctgcgcga
    15961 tgcgcgcgcg cacgtcgagc tcgaactcga tgtggcgggc cagccgttcg aagacttcgg
    16021 tcaggcgtcc gctcatctcg ccgacgcgga tcatcgcgac gtagaagccg ttgaagacat
    16081 tcgagtgccg cttcatcgcc gtggccagtt catgcccctg ttcgaggctg gcacgcagat
    16141 cgatcagcag cttgatgagc gagtccttgg ccgtcgaggc ctgcaggctg gccagtgcgc
    16201 gcaggatcgg aacgccggcc cgttgcaacg catacatctg acgcgagaag atcagcaagt
    16261 cctggctgct gaccggagac gagcccagcc cggaccagcg cgcccacggg ctggcctgtg
    16321 ctgcggagat ccgttcggtg atcgaaagca gcgtgacgcc ggtcgcctcc agccgggtgg
    16381 ctgcggcatg ggcgctttct gcctcgacct caccgttcag cgcctcgccg cgtgggttgc
    16441 gtcccgtcca tgcaaacgcc gccatttcag tcgctgtccg cgcccagtcg catcgcctcg
    16501 gccagcgagg tcgttccagc gcgcaccagt gccagtgcat ggtgagccat cgtgcggcca
    16561 cgcaactgct cctggccgag ccgggagaac gccaccgggt ccgcatgtgt tgccgcctgc
    16621 gcgaggtcgg tattcatctc cagcatctcg tagacaccga tgcggccgct gtagccgctg
    16681 ccgttgcaca gcatgcagcc acgcccgcgc agcgtgcgct cgccaactgc gtcgtcgccg
    16741 ccgagcgcgg tcagccagcc gacttcctgc ggtgtggggt catggggctc gaggcagttg
    16801 gtgcagttga cgcgcacgag gcgctgcgcg atcaccgcct gcaacgcact ggcgaccatg
    16861 aaggcgggcg cgcccatgtc cagcagccgg aagggggtgc tgatcgcatt gcgcgtgtgc
    16921 agcgacgaca gcaccaggtg tccggtgatg gccgcacgca gaccgatgtc ggcggtttcg
    16981 gcatcgcgaa tctcgcccac caggatcaca tccgggtcct ggcgcagggt ggctcgcaac
    17041 accttcgcga agctcagttc gatcttgtcg ttcacctgga cctgcgtcag tccgggaagc
    17101 cggtactcca ccgggtcctc gacggtgatg accttcagtc gctcggcatc gatctcggcg
    17161 agcgcggcgt agagcgtgct ggtcttgccg gcaccggtcg gcccggtgac cagcaccatg
    17221 ccggaggtcc gcagcaggat ctgctggaac tggttgagca ggtcgcgagg catgccgatc
    17281 tcgtccaggc ggcgcatgtc cttgccctgg ccgagcagcc gcatcaccac cgattcgcca
    17341 tggtgcgacg gcatcgtcga catccgcacg tcgatgccgc tgtcgcgcac ccgcacgctg
    17401 aaccggccgt cctgcggcat gcgcttttcc gagatgtcga ggccggccat cagcttcagg
    17461 cgctgcgcga gcgctggcgc gatgcgcttg tcggcctgcg tctgcgtctg caggacgccg
    17521 tcgacgcgac agcggatcac cagcccgccg tcctgcggct ctatgtggat gtccgaggcg
    17581 ccgatctgca cggcctcctc gaagatgctt tgcagcaggc gcacgaccgg cgcactttcg
    17641 gcgccactga tgccggtcgc cgcgccgacg tcggcgctgc tgcccacgtc cagctcgagt
    17701 gccttggcga gaccggtgat ctcgtcggtg cgccggtagt ttcgatcgag cgccggcagc
    17761 agctggctct cgggaatgac ggccacgcgc acctggcgct tcagcagccg gctgacctcg
    17821 tcgtacgcgg cgaggtcgag cgggtcgacg aagcccacga gcagttgctc gccctgatcc
    17881 tcgagcacga tgacgcgcag gcgacgggcg ggcgattcgg gcagcaggcg gacgatctcg
    17941 ccgttgaggg cgaagttctt caggttgacg gccggcacgc gcagctggcg cgccagaccg
    18001 tgtgccaccg cttcctcggt gacgaagccc gcatcgacga gctggcggcc caggcgcttg
    18061 ccgctggcgc gctgcatttc gagtgcttcg ttgagctgtg tctcggtgat gagcagctgc
    18121 ttgaccagca cgtcgccgag acgcagccgc tcgcgccgcg tcggtgtcgc agctgcgcgc
    18181 gccggcacgg gcgcggcctc ggtccgggcg ggcggcacga ctccacccca ctgcaggggt
    18241 gtcagcatct cagccatgaa ccatgggtcc tctcaagcgc tggtgtgaat gcggcgaggc
    18301 tcttcccggt aggtccgcga cggatgcccc acgaagccga tgatcggtgc gccccgcccg
    18361 gcttcaaatc gagaaatgcg gcgcctgcac cgccctgttc gacggatcgc gggcaccgat
    18421 ggcgtcactg gttgctggtc gatcgcactt cctcgatcgt cgtcacgccg gcgagcacct
    18481 tgtcgatgcc gtcctggcgc agcgtgcgca tgccttcgcg ctgcgccacc atcttcagcg
    18541 cctctgcgcg ttctcccgtc tgcaccatgc ggcgcaggtc gcgcgagatc accatcagtt
    18601 cgtgcagacc ggcgcggccc ttgaagccgg tgtggttgca gtgcttgcag ccggggctgt
    18661 ggtacctgac cggacggccg tcgctgccgt gctgttcggt ccattgcgcc agcagcgcgt
    18721 cgcggccggg ttgctcttgc gcctccagtt cgaaggcatg caggtaatcg tcgagcagtt
    18781 catcgatctc ggccgacgtg gccgggcggc tctggcggca ttcagtgcac agccggcgca
    18841 ccaggcgctg cgccagcacc gcgagcagcg agtcggcgaa gttgaacggg tccatgccca
    18901 tgtcgagcag gcgcgtcacc gtctcgggtg cactgttcgt gtgcagcgtg ctcatcacca
    18961 ggtgaccggt cagagaggcc tcgatcgcca tctgcgcggt ttcctcgtca cggatctcgc
    19021 cgaccatgat cacgtccggg tcggcgcgca ggaacgcgcg cagtgccttc gcgaaggtcc
    19081 agtcgatgcg cggattgacc tgcacctgac gcagcccggg ctgggtgatc tcgatcggat
    19141 cttcggccgt ccagatcttg cgctgcggct ggttgatggc ggccagtgcc gagtgcagcg
    19201 tggtcgtctt gcccgagccg gtcgggccca cgcacagcac catgccgtag gggcgctcga
    19261 tcgcggcttt cagctgctcg agattgcgcg ccgacatgcc gagctgctcc atcgggatcg
    19321 gccgtgccga caccaggatg cgcatcacca cgtcctcgag gctgttgttg gtcgggatcg
    19381 tggccacccg caactcgatg cggtgctgcg acgagaagcg ggcaaagttg atcttgccgt
    19441 cctgcggctt gcggcgctcc gagatgtcga ggtcgcacat gatcttgatg cgcgcgatca
    19501 gcgacgagcg gtagttaggc ggcagctcga ggtaggtgcg cagcacgccg tccttgcgga
    19561 atcggatgcg gatcttctcg cgacccggat agctctcgat gtggatgtcc gaggcgcctt
    19621 ccttgtaggc ctcgatgatc atgttgttga tcaggcgcac cagcgagttg tccgactgct
    19681 cgatcggccg gtcgtcggtg atggagtcgt cgttctcgcg ctccagcgtc tcgaccagct
    19741 ggtcggtgcc catcggctcg atcacctgcg tgtcctgccc gaagtcgccc ttgtggcgcc
    19801 ccgacgcgac cgcgccgatc tgctcgtaga cggcatggat gcgctggtcg atcgagcggc
    19861 tctgggccag caccggcagc accttcgtct gcaccgagaa ctcggcttcc tcgaccgccg
    19921 aatgcagtcg ggccggatcg tcgatcgcga tcaccacccg gttgtcgcgc atcagcaagg
    19981 gcaacaccct cagccgcgtg gcaaccgcgt acggcagctt gcgcagcgcc tcgctgtcga
    20041 ccgggaacgc gtcgacgtcg accagcggat aacccatctt gcgcgccatc gccacctgca
    20101 ggtcggcacg cgacacgatg ccgttgcgca ccaggatctc gccgagtgga acggcgcgat
    20161 cttcctgctg ctgcttcaac gcttcctgaa gctcatcctc gctgaccagt cccatggaca
    20221 gcagtgcttc gccgatgcgc accatcggca tgcgagcctg ctgatcgagc gctcgcgtca
    20281 gctgctcggg cgtgacgatg ttgcgggaga ccaggatgtc ccccaccttc ttgttgcgca
    20341 gttgtgcctg ctgcgctgcg gcctgctcga cctgctgcgg cgtggccgag cgttgctcga
    20401 ccagcacctc gccgatgcgc gggcccagtt cgaagctgct gaatgcggcg gccggaacga
    20461 acatccgggt gactccctgg atggcgccgg tcttcgagcc gtcttgagga ggaaacagga
    20521 acaggccgaa gggcgtctcc acatgtccga gcgttaagcc gaccagctcg ccgccctgct
    20581 tccaagtgat cctgaatgcg gtggccgtgc cgggggcgag cagcagtgct gccgggtcgt
    20641 cctggtcgat ggagggtgcg gtcagcggct gcgtcaggca caggcagcgg aattgatcga
    20701 agcgcagcgg caaggtcgtg cgcgaaggcg gcaactgcac cagcgcggcg ccctgatcgg
    20761 gatgcagcgc gctcagctgg ccgtccatca gcttgtcgtt cagaccggtg atggtgcagg
    20821 cgagcgcttc ggtctgcggc tgcacctggg ggtaggccgc gaacgggggg gacggccagc
    20881 agaaggccgt cgggtggcct gcggcggacc cttccggtgg tcggtctccg ccccagtgca
    20941 gcggtgtcag catctcggcc atgtcttcct cggcgtggtt caacggaatt tcacgcctga
    21001 tgatccgtgc gatggcgaga tcgcaacgcg gcgattagca gggtgcgagg cgcccagatc
    21061 cgcgcttagc ctgtggaggg gctggcggaa cctgtttcag gtcggcgcgt aacgagtcac
    21121 cggcgacatg gccgtggctg gtgatctcgt ggaacggggg gttcgaggtc cgggcgcgcg
    21181 cgcctggggg gaatcctcag cgctgcatca gcttgcgggc gagcctgatc accgaccacg
    21241 cggacagggc cacgcgcgcg atgcgccaac cgatccggcg ccgccgtgcc agcagcaaca
    21301 gcagcgggat gccggccgcg gctgccgggg cgagccacga acgcgatcgg tcgaccgctg
    21361 ccgtgagact ggtgagtgga gcggtgctgg cctgccactg ggctcgaagc gactcgcgct
    21421 gcgacgctgc gcgcagcgaa agcagttcgc gtcgcagcgc aagctcggcc agtcgcacgt
    21481 cgttgtccat cagggctccg caggcgggtt cgacgaggca cgcagtgcgg cgatgtcgcg
    21541 ctgcagttcg gccaccgtga ccgacatgaa tggcggccgg ctcgcggcac gagcgcgcca
    21601 acggttcact gagatccatg taccgccgac gtaggccagc gccagcccac cgacggtgag
    21661 caggcggtgg ctgtcccaca tcgccacgac gacgaatgcc gtgcacatca cgaccgccat
    21721 gcccaagagc atcagcgtga tggctgccga aatcagcagc ccgaccagcc gcagccgctc
    21781 ttcctcgagt tcggtcgagg ccagttcgag ccgcgtggcg ccgatgtcga gcagcagcgc
    21841 cagcagcgat cgcaccgatt cgaacatgcg cagggtctgc gtgatccgct cagcggcggg
    21901 cgatcagcat gccgatcagc aacccgactc cggcggcgac acccatcgct gcgtagggat
    21961 gctcatggac cgcgcggtcg gtgacacgcg cggcatgccg cgcattcacc atcgcggtct
    22021 cctcgaggcg ctgcaactcg tccttggcgc gcaccagctt ctcctgggca cgcttgagca
    22081 gggtggcgta ttcgtggctg gcggcagcac cagcaccttt cagcaacgac tcggcctcgc
    22141 ccaccgtctg cttgagtgcg tcggcgagtt cgtcacgggt ggcttgcagg ctggcggcgg
    22201 cagtatcgat ctggctcatg ggagtctccc ggtggttgag ggtctgtccc tttgagatgg
    22261 ggacgttggc gaccctgtca aggggtactt tggcactttc gacccggtgc agccgttgat
    22321 cccgatcaac ggaagccggg attccctgct gactgcgagg tcaggccaca ggcctgtctc
    22381 agtcgcgcgg cttgtagacg atcgtcatcg gagacaccac ccggccggtt tcgtcgcgcg
    22441 gcagcaccgc ggtcttgtcg atcccgcgct tcactgcgtc gtcccattcc ggtacgccgc
    22501 tggccttgac gatgcggctg gacatgatgc ggccgtccgg tgccacggtg atctcgacct
    22561 cgaccgccgg gttgccaggc aaggtgtccg ggaacgacac gttcggacgg atccggccct
    22621 tgatccggcc gatgtagtcg gcgctcgggc cggcgctctg cgccgccgtg ccggtggcgt
    22681 tggctgcgcc ggtggcgccg gccaggctgg cgatgcgctg catctgcttt tcgcgctcgg
    22741 ccgcgttcga ggcctcgacg gcggcgtcgc gcttcttgtt cgccagctcg gtcttcagct
    22801 tctcggcggc ctgctccttg cgcttctgct cggccaattt ctcggcctgc tcggccttgc
    22861 gctgcctggc gagcttctcg gcttccttct tcgcttcctt ttcctcgcgg gcctgctcag
    22921 cgagttcctt cttgcgcttg tcttcgaggc gtttggcctt ctcgatagcg atctgcgcgt
    22981 cgggcaccgg tgctgcctct ttaggtggcg gctttgccgg ttccggctcg ggcttcgccg
    23041 tgcgaactgg cggcggcggt ggcggctcca cctcgaccgg ccgcggcgcg gcgatctgcg
    23101 gtaccgcggc ccacagttcg gcttcgacgc cttcgggcga gctgacgcgc cagttgacgc
    23161 tgaaggtcag cgcagcgacc agcagcaggt gtgcgatgac ggccatcgcc agccctctgc
    23221 ccaggccggc gggccggcgc ggcatgaacg cgtcgcgaga gaaggtggcg gttgcggcca
    23281 tcgtcatgcg cccggttgct ggatcagccg ccgttcttga ccgacaggcc gacgcgctgc
    23341 acgcccgatt tctgcaatcg gttcatcacg tcgacgacgg tcttgtactt gaccgactga
    23401 tcggccgaga tcaccaccgg cgtgttcgca tcgccggcct gcagatcgag cacgcgggcg
    23461 gcgatcctgt ccagcgcgac cgcctggccc tcgtccttgc tgtcgacgcg tatgcggatg
    23521 cgctcgtccc ggccgacgat caactcgacg acgtgctcgg gccgctgctt gctcttgccc
    23581 accgtcggca gatcgacgac accggtcgtg atcagcggcg cactgaccat gaagatgatc
    23641 agcagcacca gcatgacgtc gatgaacggc accatgttga tctcgttgat ggtgcggcgg
    23701 cgtccgccgc cgcggtggct gacggccggc atctcagcgt cccccggtca tcgaaggtgc
    23761 cggcgcgggt tgcccgaggt tgcgctgcag gatgttggag aactcctcga tgaaggtctc
    23821 catctggatt gccaggcgat cgatgtcgcg cgcgaagcgg ttgtaggcga tcaccgcggg
    23881 gatggccgcg aacaggccga tcgcggtggc gaccagcgct tcggcgatgc ccggcgccac
    23941 ggtggccagc gtcacctgcg tcaggttcgc caggccgatg aatgcgtgca tgattcccca
    24001 gaccgtaccg aacaggccga cgtacggcga caccgagccg accgaggcca ggaaggacag
    24061 gttcgattcg atcgtgtcga gctcgcgctg gaagctcgcg cgcatcgcgc ggcgcgcgcc
    24121 gtcgagcaat gcacccgagt cggtgacgcg gcgctcgcgc agcttcatga actcgcgcat
    24181 gcccgacgcg aagatccgct ccatcggggc cgacgaggtg ctgcgcttgg tggcttcgtc
    24241 gtacaggtcg ttgaggttct tgccggccca gaagtcgcgc tcgaactcct cgttctcgcc
    24301 gcgcaccttg cgcaggccga acagcttgct gaagatgacc gtccagctcg ccagcgaggt
    24361 gatcagcagg cccgccatca cgagctgcac caccacgctg gcatgcagca ccaggctcaa
    24421 tatcgacatg tcttggttca tggagtcggt tcgatcagct tttgggaatc ggtgtcgcgg
    24481 tcggcgccgg caggcgcgcg gcgatctcgg tcggaatgcg gcgaggctgg aaggttccgg
    24541 cctcgacgca ggcgatgcgg atgctgcctt cggtcagcag cacgtcgtcg cgccaggcct
    24601 gctgatcgag cgtgatcgag gtgcgggtga cgtgggccag ccgcaccgtg atcctgagca
    24661 ggtcgtcgag ccgggcgggc cgacggtagc gcatcgtggt gtcgctgacg acgaacatcg
    24721 cgccgccgtc cacgcgcatt cgttcctggc cgtgacccag cgcgcgcagc cactcggtgc
    24781 gggcgcgttc gaagaacttc aggtagttgg cgtagaagac gacgccgcct gcgtcggtgt
    24841 cttcccagta gacgcgcagc gtgaattcga aatcgacggt cgtcatgcca ggtgcgaacc
    24901 ggcgcagtgg ccacggtcat gcaggccacg aaacctcaag gttgcacccg cttgtagacc
    24961 gggccccaca gaggatggcc cgcctccgac ttgccgagcg cgaaattggg atcggccgct
    25021 ttcgccgcac gaaaggacgc ttcgcactcg gtcaggcggt tgctggtgca gtagatgaag
    25081 gcgaggtgct tggcggcggc ggcccggtcc ttgggcgagg gcagtccgag cttcagcgcc
    25141 aggctgaact gcttctcggc gtcggtgtac tgcgcatcct cgtaggcgcg cagcccggcc
    25201 agcaaggcct gctcggctgg ccgctcgacg acgtcgagca ggcccggccg cggcggcggc
    25261 gacatcgcgc agccggcgac cagcagcgcc aggacgaaag gagacaggga tttcatgatc
    25321 cgaacttgtg cttgatcgtc accggctggt cggcggtgac ctgaaccgtc gcactgtacg
    25381 gtggaaagtc ggcattcctg atcgtgatct ggtgtcgacc ctcgggcagg gtcagttcgt
    25441 tcaacggcgg tgccgtgccc gccggcgctc catcgacctc gatctggccc cacgggctga
    25501 tggcgagccg gacgcgtccc ttggacatga ccgccgctgc ggcgccggtg cggccggcag
    25561 cgtcgcgagc ccgggcgtcg gcttgggtct tgtcacggcg cggccggtct gccgcagcgg
    25621 gccggggtga tgcgtccagg gccgacgatg ccgcaggctg ctgcgacgac ggtgccgcgt
    25681 tcgcctgctc actcgcggaa accgtctgcg ccacaggttg ctgcgccgcc gatgccgcgg
    25741 cggatgcagc gctcgacgcc ctgccgatcc tgcgctgctc gttccaggcg ccgagcgtca
    25801 gggcaccgat gccaaccaag ccgatcaacg caccggcgag cgccgccttg tggttgccca
    25861 gccgcttcca cacactcggc gccgcgtcat ctccggttcc ggacgcggcg aaatgccggc
    25921 gcaactcgcg cgacatcgca gaggccgatt cgaagcgctt gtcgacatcc ttctccatcg
    25981 cacgggcggt gatgtcggag agcaccttcg ggacggcgcg gttcacgcgg tgcgccggca
    26041 gcggcgtgtg ctccagcacc gccgtggtga tctgctccag cccgacgccg gtgaacggct
    26101 tcgatccggt cagcaactcg tagagcacga cgcccagcga gaagacgtcc gaccggcgat
    26161 cgacctcctg ctgacgcgcc tgttccggcg acatgtaata aggcgagccg gctgcgatgt
    26221 cgctgcccgg cgggctgtca cggcggtggg cgatgcgcgc gatgccgaag tccagcacgc
    26281 agggctggcc gcgaccgacc atgaagatgt tggccggctt gatgtcgagg tggacgatgc
    26341 ccttgcggtg cgcgtaggcc agcgcatcgg ccacacggcc gaccagcacc gctgcctgcg
    26401 ccgggctcgg ccgccaaccg ctttcgcgca gctggcgcag gtcctgccct ttgagcagcg
    26461 ccatcgcgat gtaggcatgg ccttcactga tgcctgcgtc gaacaccgtg acgatgttcg
    26521 ggtgcgacag gccggccgcc gcgcgagcct cgttcaggaa cagcgcattg aacgactcgc
    26581 gctgctcggg ttcgatttcc aggtgcagcg tcttgatcgc gatcacccgc gacagcagtg
    26641 gatcgtaggc cgcgtagacg gtgccgagcc cgcccttgcc gatctggtac ttcagcgcgt
    26701 agcgcccgat gaagccgatc gacggcagcg actcctcggg cgtcggtgcg gcgtcggccg
    26761 cggccggtgg gcggccccct tcgtccggac cgttgtagcc ggcgacggtg tcgggtggcg
    26821 ggggggcgga tgaactcacg gggcgagggt agtcgatcac aacgcctgtt tcaggcggtc
    26881 catcgcctgc tgcaattgcg tcatcgagtt ggcataagac aggcgcacat accgggacgg
    26941 ttcatggcgc ccgaagtcgc gccccggcgt catggccacc tgggcgcgcc gcatcaggtc
    27001 gaaggtccag tcccagcttc cgcccggcag gtgagcggcg tgggccgagc aatccgccca
    27061 tgcgaagaac gcaccgtccg gctcgacggg caccgtgagc ccgagcgcat tcagcgccgg
    27121 cacgaggaaa tcgcggcgtg cccggaaagc ctgccgtcgc cgctcgtatt cggccagcga
    27181 gtcgggttcg aagcaggcca tggccgcgtg ctgcgcgatc gccgaggggc agatgaacag
    27241 gttctgggcg agtttctcga ccgacgtcac cagcgactcc ggaaccacca gccagcccag
    27301 ccgccagccg gtcatgttga agtacttcga gaagctgttg accgagatca cgtcgtcgcc
    27361 atggcgcagt gcggagtcgc cgtagcgctc gtcgtagctg aggccgaggt agatctcgtc
    27421 gacgatcgtg aatccgccgc gcgcgcgcac cgcatcgacg atgcgcccca tctctgccgg
    27481 atcgatcgag gtgccggtgg gattcgacgg tgaggccagc agcacgccgc gcgtcttcgc
    27541 gttccacgcc tgcagcaccg ccgcggcgtt gagctggaaa cgatcggcgg gtcccgtcgg
    27601 gaccagcacc gcgacgccgt cggcagccgc cacgaaatgc cggttgcacg gatagctggg
    27661 atcgggcagc agcacttcgt cacccgggtc gatcagcgcc aggcaggcca gttgcagcgc
    27721 ggccgacgcg ccggcggtga cgacgatgcg ttcgggggcg atgtcgagcc cgaaacgttg
    27781 cgcgtaccaa cgtgcaatgc gctcgcgcag cggctgcagg ccgatggcat cggtgtactg
    27841 ggtgttgccg tctcggatgg cccgttgcgc ggcttcctgc accagtgcag gcgcggtgaa
    27901 atcgggttca ccgatgttca ggaagatcat tggccggccg ccctggctgg cgtcgcacac
    27961 cggactgcgc gccagttcgg atgcggcctt ggcgcactcc atcacgtaga aaggctcgat
    28021 gtgcgacagg cgcttcgaga gcctcatgcc ggaccgcgtg cgggtggacg gtggagtcag
    28081 cggcgtgcgg cccgcgccgc gtcgacctcg accggccgca ggtcggccgc ggtcttgtcg
    28141 atcacgccgt tgacgtactt gtggccgtcg gtgccgccgt agctcttcgc cagttcgacg
    28201 gcctcgttga tgatcacgcg gtacgggatg tcgatgcagt gcgtcagctc gtaggcgccg
    28261 atcatcagca cgccgtgctc gatcggcgac aactggctgg tcttgcggtc gacatggcgg
    28321 gccagcgcgg catcgatcgg tgcagcctcg gcgatgcacc cacgcagcag catctcgaag
    28381 tgcgccgcgt cacatcggtc gaatccctcc tgctcgcgca catgcgccga caccacatcc
    28441 ggtgattcgc ccgacagcag ccattcgtac aggccctgca gcgcgaactc gcgcgagcgg
    28501 cggcgtgtcg acttctgcgt ggccttcttg gcagccttcg ccggcgcgcc aggtttgctg
    28561 gtttcagcgg gcttctgttc ggcttcggtc acgacaaatc ttccatcagg tgggccatct
    28621 cgatcgccac acgggcggcg tcgcgggcct tgtcttcaac ccgttcccag gcctgctcct
    28681 cggtttcgac ggtcaggatc gcattggcca ccggtaactg gtgatccagc gacacccgcg
    28741 tcacgcctgc accgctttcg ttggcgacga gttcgaagtg gtaggtctcg cctcgcacga
    28801 tgcagccgat ggcaatcagc gcatcgaaat cgccgccgtc ggccatggcc atcagcgcga
    28861 ccgggatctc gagcgcgccg ggcacgctga catggtcgat cagctcggca tcgacaccga
    28921 ggcggcgaag ttcggcgatg cacaccgccg ccatgcgctg cgtgatcgcg gcgttgaatc
    28981 gggcctgcac gatgccgatg cgcaggtcgg ctccatcgag ttcggcgtcg gaaggaccgc
    29041 cctggtcagc accctgcatg cggccctcct gtcgatcgat cggtcttcat cagacccggc
    29101 ccgcggtgtc ggctgcgacg aagccggtga cttcgaggcc gtaaccgacc atgctcggca
    29161 tgcgtcgcgg gctgccgagc agcttcatgc gcgagacacc cagctctcgc aggatctgtg
    29221 ctccgatgcc gtaggtgcgc aggtccatct ggctctgggt gcgcacggca gattcgccag
    29281 ctggcttcag cttgccgagc agcgcgtcgg aaccttcgcc gcagttcagc agcaccgcga
    29341 cgccgcgctc gctggcctgc aactgcgcca gcgcgcgcgg cagcggccac gaatgaccgc
    29401 aggctccggc gtcgagcagg tccatcaccg acagcggctc gtgcacccgc accagcacct
    29461 cgtcgcgcgg cgtccattga ccatgggtca gcgccatgtg cagaccgccg gtgcgatcga
    29521 tgaacgaagt gcagtcgaac tcgccctgcg cagtcttcag cttgcgcctg ccggtgcgtt
    29581 cgatcagcgt ctcgttgcgg ctgcggtact cgatcaggtc ggcgatggtg ccgatcttga
    29641 gtccgtgctg ctgcgcgaag acctcgaggt cgggcagccg ggccatcgtg ccgtcgtcgt
    29701 tcatgatctc gcagatcacc gccgccggcg acaggccggc catcgccgac aggtcgcagc
    29761 cggcttcggt gtgaccggcg cgcatcagca cgccgccgtc ctgcgcctgc aacggaaaga
    29821 tgtgccccgg ctgcaccagg tcgctggcct tcgcgtcgcg ggccaccgcc gcctgcaccg
    29881 tgcgggcgcg gtcggccgcg gagatgccgg tggtcacgcc cgtggcggct tcgatcgaca
    29941 cggtgaacgc ggtgccgtgc tgcgcgccgt tgcggctggc catcggcggc aactgcagtc
    30001 gctcgcatcg ttcgcgcgtc agcgtcaggc agatcaggcc gcgcccgaag cgggccatga
    30061 agttgacggt ctcggctgtc acgtgttcgg cagccatgac gagatcgcct tcgttctcgc
    30121 ggtcttcctc gtcgacgagg atgaccatgc ggccggcggc gagctcggtc aggagctcgg
    30181 gaatggggga aatcgccatc gacggattgt aggttgcggg tccgcagacc ccacgctcaa
    30241 gctggccgtg cgacgcaggc caccgattgc atgcgctcga cgtagcgcgc gatcagatcg
    30301 atctcgaggt tgacggtgcc gccgggcttc agatgtccga gtgacgtgtt ctcgatggtg
    30361 tgcgggatca ggttgatgcc gatctcgcag ccggtcgggt ggtctgcgac ggtgttgacc
    30421 gtcaggctga cgccgttgac cgtgatcgag cccttgtagg ccaggaattt cgccaggtcg
    30481 gcaggcgcat cgatcttcag ggtccatgac tcgccgaccg gtgcgaaatg cgtgacgcgg
    30541 ccgatgccat ccacgtggcc gctgaccagg tgccccccca ggcgatcgtt ggcgcgcagt
    30601 gccttttcga ggttgacggg cccgagcgag tcgagaccgg cggtcttgtc caggctctcg
    30661 gccgacacgt cgaccgtgaa gaccgagcgc gcagcgtcga gcgacgtgac cgtcatgcat
    30721 gcgccgttga tcgcgatgct gtcgcccgga ccgacgtcgt cgaggtaatg cgcgggcgca
    30781 tcgacatgga gccggttgcc gtgagaggcg gcgtcgccca gcggcttgac gtcggagatc
    30841 cggccgacgc cggtgatgat gcctgtgaac atgcggctat tgtctcagcg caacttcgga
    30901 cgggccagca cccgcaggtc gtctccgatg cgctcgacct ggtgaaaccg cagctccacg
    30961 gcctcagaca ggtcggtcag gggcccgaaa gcggcgagat cgcgtccggc accgagcagc
    31021 ttgggtgcca ggtagatcag gaactcgtcg accaggcgct cgcggacgaa cgatccgttg
    31081 agcttgtggc cggcctcgac atgcagctcg ttgatgccgc gcgctgcgag atcgtccagc
    31141 atcgccgcca ggtcgacctt gtcgttgacg cccgggaccc gggccacgtc gattccacga
    31201 tcggcgaagg cggcgaggcg ttcggtggtg gggtgggcag cgtagaccag caccgagccc
    31261 ggcgcctcga agaggcgggc gccgagcggt gcatcgagcc gcgaatcgac gacgacgcgc
    31321 agcggctgcc gcggcgtggg caccagccgg acgtcgagcc gggggttgtc ctcgaccacg
    31381 gtgccgacgc cagtcagcac cgcgccggcc cggcggcgcc aggcatggcc gtcggtgcgc
    31441 gcggcctcgc ccgtgatcca ctggctgacg ccgttgggca gcgcggtgcg tccgtcgagc
    31501 gatgcggcga tcttcatccg cacccacggg cgctggcgtt caattcgcga gaagaatccg
    31561 atgttgagtt cgcgcgcggc gtcgacggtc tggggcgaca cgccctgcgt ggtcacttcg
    31621 acgccggtgg cacgcagccg ggccgcgccc tggcccgcga ccagcgggtt cgggtcttcg
    31681 agggccatca cgacgcgtgc gatgccggcc tcggcgagtg catcgcagca gggcggtgtt
    31741 cggccatggt gggcgcaggg ttcgagcgag acgacggcgg tggctccgcg caccgaatgg
    31801 ccctgcccgg cggcgtcgcg cagcgccatc acctcggcgt gcggtccgcc ggcctgctga
    31861 gtgtggccgc ggccgatcac ctgcccgtcg gctgcgacga tcaggcagcc gacgcgcggg
    31921 ttcggctcgc tcagcgcgat ggcctgctgc gccagcgcga gcgcctcggc catccagtcg
    31981 tcatggcggt gcgtgaaatc ggtgggcgtg gatggcgtcg atgtcatctg ggtcggatcg
    32041 cggggtcagg gctggtgttg aatcgtggcg acgcgcgagg tgggcggcga cccgacggca
    32101 tccgccattc tcggggcagc gccatccggg caggccagat cgccgcggat caccaggaag
    32161 ttctgttgca gcagcgcgcg atccacaccc aggtagtcgg cgggatgctc ctcgttgcgg
    32221 tcgatcgccc cgctgccgtc gtgatcccag gcgtagcggc agacccggcg gtccgctgcg
    32281 gtggtgccga tcgtccagcc gaagggaacc agcgtgctgg ttccggacca gcgcggcgga
    32341 tcgccggcgg cgggcaccag acagttgtag gcgacgaatc gttcaccgag gtcggtccat
    32401 gccgccgcgc cgacgcgggc gggttcggcg gccagcggca ccgcgacacg gtggatgccg
    32461 tcgccggagc gatacagcac ggacttctgt gcctcggtgc cgcaccaggg atctgccgcg
    32521 gcaacggcgc tggccagcgc gacccccatt gccagctcga gtggcggatc gttggcggcc
    32581 agcgggtcgg gcgggttcgc attcgagaat cgcaccgtgc cgctcagcag caggccccgg
    32641 gcctcgctgc aatgggtcag gtgctcggcg gtcagatccc cgttggacag gccggcgggc
    32701 acttcggagc actgctgggt cacctctccg ctgaggttgt cgagcacgaa ggccgtgctg
    32761 cccgccagga cgggtttcag gacactgcgt ccgttgccca ggttcttcgc ggtgagcggg
    32821 atgcgcgccg agcgcgccat gcccccggag gctgtcagcg cgctcggacc cagcgcgagc
    32881 gcgccggaca gtgccgggtc ctggccggcg atgatcgtgc tgacctcggc ccggtgctcc
    32941 gtgccgtcgc gcgcggtcca tgcgacgccg acggtggcgt gcttcatgcg aaacgccgat
    33001 ccggagtcga cttgccgggt cagttggaac gtcgtgttga gccgcagtcc gccgagttga
    33061 tccagcgacg aggaagcggg ctcgatgcgt tcgtaggatg acgcggcggg tggcgcccct
    33121 gcgggctgca ccagcgatgt gtaggctcgc accgtctcga tgtcttcctg cgcgagccgc
    33181 agggcctgcg agcgttgccg cgccagatcg gcatgggcct gcaggtggtg gtgcagcctg
    33241 gacatcgaca gcacgccgag cgcgatcacc aggaaggaca gcagtgcctc gatcagcgtg
    33301 acgccgcgct gtgatcgtgg ccgagggtgc cggaatgcag gtgtagtgct catcagaagt
    33361 ccctccagct gccgggcaca cgcgcatagc tgccggtctg ccgttgcagg ctgcgcagca
    33421 gcgtcgcgtc gtggatcagg tcgggggcgg cgctgccgcc atagcccgac tcggaaatga
    33481 ccgcgccgtg gacgcgcccg cgcgggggcc cagcatcgtc ccagtggacg gcggatgcgt
    33541 agagcagtcc gtgcagcgtc acgtcaccgg tcagcgtgat gctgccgtcg accacgatga
    33601 ccaccggtcg gtcgggcgag cccagagtga cgggaccttc gattcgtgcg tcgccttcga
    33661 tcgagatcaa ccggttgctg ctgacgctgt cgatcgctgc tgcgagcgga actccgcact
    33721 cggcgtcgca cgctacagcc tgcaccgcgg cgtgaccgtt ccaggtggcc ttgtccagtc
    33781 cgaagtgagt ggcaaacagg cgcgccgggg ccatcgatgc cagtgccgcg tcgtgtgtcg
    33841 atgcagagac cgcggctgca gcgccgggtg cggtggtcag ccgcagggcc gatccgatca
    33901 ggctgccgcc ggtgtgaacc gtgatgccgc cactggccgc atcggtgtgg tgcagcccga
    33961 gcgcagccgc gcctgcatcg accgaaccgc gggtcgtcag cggcgcggcc ggcggactgg
    34021 acagtccggg cagcagaccg aggacgacct gcaaccgaac cgatgcatcg ggacgccccg
    34081 ccgcacccgg gcggcacggt ccggccgcgc tggtgcagcc cgtcgaggtg acgcggatca
    34141 gacctgagcc tgcctcggta tcgaactgca cggagtaggc cgggcgcggc gtgccatcgt
    34201 cgctttcgat gacgtcgagc atggggtggc cggctgcggg gcaactgcac gcccagcctt
    34261 cggcaccgag cacgcatgcg ggttgcagtg cgaccggcgt gccaccagac atccacgtca
    34321 ccggcgcctg ctgtcctgtc acgtggtcga tgcggacgaa gcgttcgcgg aacgacgggt
    34381 cggtggcggt gctgctcggc aggcaccgtt catcgatgcg ctggggatgg ttgagctgcg
    34441 cgatggccca ctcgagtccg gcctcggcgg cctcgaatgc ctgcgcggcg cggtactggt
    34501 tggccgacgc ccgttgctcg aacaccagcc cgcggttggc gtaggcggct gccagaagca
    34561 tcgcgaagaa caacagcagc gtcaccggca aggcggccag cccgcgttgc cggcgtgcgg
    34621 tcggggtttc ggtccgggat gggtgcatcg tggcctcacg ccgggcagct gcccgtgact
    34681 tcgtcgttgc gcagccggac gctgctgcgc acgatgcgtt cgctggcggc atccgtcgtg
    34741 ctgcgcgcag cgaccgtcac ggtcacgctg cgcacatgca ggcggggcgg gcaggtggcg
    34801 gacggcacgg ggcagggcac ctgacatgag tcgccgaggt ccacgacctg ttctgtaggc
    34861 gcgatgtcca ggcgcgtgac cttcattgcg ttgggatcgg tcagcgcctg ccagcgcccg
    34921 gcgctcaatt gcagttcgag cgttccgccg cgcaggcggt agccgaactg ctcgttgtcg
    34981 tcgacgctgc cgttctcgat ggcgtcgcgc gagaagcgga acgtgacggc aggcgctggc
    35041 agcctggaga ccgccatcgc gctgtacggg ttggaggctg cgccgcttgc gcccgttgcc
    35101 ggatcgcgcg gccacacgcc ggcggccgca tcgccccagt agccggcgcg gcgcagatcg
    35161 cgcgtgatga ggtcggtggt ggcgcgcagg tcctgcatca gccggctctg cgctatcagc
    35221 gaccggcttt cgcgcagatg cacgacgaac gccgtgatgc ccgcgcccgt gatgaacagc
    35281 ccgagcgtga tgccgatcag cagttcgacc agcgacatgc ccgcctgacc cttggcgctg
    35341 ggattgaccg gcgcacgcat gtcacaggct ccagcaccgg cggttgaccg acgacgcgtt
    35401 gtcggccgca tccgtcgcac ccgacgcgta gacgacgttc aacccgtcga cgctgagccg
    35461 caggtggcgg cacgtcgcgt cgcgttgctg gctgccgatc gcggacgcgt gaaccgcgta
    35521 gccggtcgcg gtgtgctcca gcatctcgat ccggtagtgg cgggccggcg aggtggcggc
    35581 caggcccagc tgcgccagat cgccgtagct tggatggtcg gcccggaagc gctcctgctg
    35641 caactgaacc ttcatcaagg cgatcagtgc atcggaccgg cgggtcgtcg cgatcgtgct
    35701 actgaacgcg gggtaggcca ccgacgagag gacgccgatc accgagatgc agatcatcag
    35761 ctcgatcagc gtgaagccgg cgacgtggcg cggccgtgtc cgccgaggcg gtgtcgagat
    35821 cggggcaggc agtggcatgg atggctcctg gttgtgtgaa cgtcagcaga cgcggtagcc
    35881 cggtatcgcc ggggccgggc cgtcgggaga acaggtgcgc acgcgcccca gcacattcac
    35941 gacgtggtgg atcgaaccga cagccggcga tctcacgcgc agcgtcccgg tgggtgtgct
    36001 ggtgccgtgc agcgggtcga acaggatcga cgacacattg gcctgcacgc tcgcgccggc
    36061 ggacgtgggg acgcgcacgg tcttgatctg cgtggcatcc tcgatgcagg tcgcctcgct
    36121 cgacccggca gtgcaggcgc actggttgcg cgcgccgctg tgcacgacat agcagctgcc
    36181 ccaggcctcg ctgtagaagc tcaggcgcag cgcctcgttg cgcaggatcg attcgctgcg
    36241 cgtgaactgc acatccgtgg ccagctgcgc tgccagtccg gccagtcgct gccgctcgat
    36301 caggccatgg aagctcggag cggcgccgca ggccagcacc gcgaggatcg caagcgtggc
    36361 cgctgcttcg atcagcgtga agccgggttg ccgacgcagc cgttgcgagg ccgtgtgccg
    36421 aggtgtgtgt tgcagcgaat ccatgggttc cactgtagga accacgcagt cgcggcgcga
    36481 tccccgggag gtgggtcgag ccgttcggcg aagggggccc acggaagggg gctgccgagg
    36541 gccctcccgc ggactgctct tgaaatgcct gacagaccga cgtgatcggc gcagtcgctc
    36601 gataccggca ttccgtctgt tcgctggcgc acagaccgcc gtgctgaacc ctgggaaatt
    36661 ggcgctcccc ggcgggcata cctgacggca tctcaacagc gtaccgatca acatggatct
    36721 cacacgcgtc ctgatcgtcg atgacaacga catgttcatc gagatggcgt cattcattct
    36781 gggcgccgca gcctacgtgg tcgagtccgc atccgacgcg agccaggcac tcttgaagct
    36841 gccggtgttc ttgcctgatc tgatcctgat ggatgtgcag atgcccgtga tggatggcat
    36901 cgaactgaca aggcacttga aagcggatcc ggcaaccagg cacatcgtga tcgtggcgtt
    36961 caccgcgttt gcactccagg gagatgcgaa gaagctgcag gacgccgggt tcgatggcta
    37021 catcggcaag ccgctggacg tgatgacgct ggccgccgaa gtccgcttct ggctagaggg
    37081 cgcgggcagc gtgcgagaca accgctttgt ctggccctga gcgcgacggc ccgagtcgcc
    37141 atgagatctc agcgacgcgc ggtgcggatg ccgaaggggt tctcatgacg aatggcatac
    37201 agctgccatt cccttacagc atcaagcgac acggcatatc gctgtcgaac tgtgaagacg
    37261 aacccgtcca gacccccggg tgtatccagg cacacggcat gctgctggcg gtgcgcatcg
    37321 acgatctgat cgtcacgcag gtgagcgaaa actgcgaacg ctggaccgcc ttgcgcgtcg
    37381 atcaggtgtt gggcagcgcc ttgtcgcggg tcgtcggatc cgcctctgcc gagcgcatcg
    37441 gggaactggt acgcaccgag gcgctggatc aaaacccctg ctatgcgctg acttccagcc
    37501 tgcccggctc gcccgcaggt gccgcggtga tggacatgac catccacacc gccgatggcg
    37561 tgctcctcgt cgaactggag cccagcgggc gggtgcttcc cacctgtggt caggacggcg
    37621 actacttctc gatggtcagg aagaccatcg gtcgtctcaa gagcacccgc tcgctggcgg
    37681 agttctgcga tgtggtcgcc cgcgaggcgc gcagcatcac tggcatggac cgggccatgg
    37741 tctaccactt ccataccgac gacagcggcg aagtgatggc cgatgcccgg cgcgaagacc
    37801 ttccgtcctg gcaaggcctg cgctatccgg ctgcagacat tccgaagccg gcccgcgaga
    37861 tcttcaagag gataggtgtc agacccttgc ctgacgccca gggcgaactg tgcgagatgg
    37921 tgccgctgct caacccggac acgcagcgtc cgttgcagat gacccattgc gccttgcgtg
    37981 gtgcgtcggt gatgtacacc gagtacctgc agaacatggg cgtggcggcg acgctgacga
    38041 tgccgatcct gcgcgatggc gagctctggg ggctgatcgc ctgccatcac tacacggcca
    38101 aggcgcttcc gtatccgctt cgatctgcgg ccgagtttct ggcgcagatc gcgtcgctgg
    38161 aaatagctca ggccgagttg cgcgagcacc tgcaataccg gatcgatctg gacgcggtgc
    38221 atctgtcggt gctggcgcgg gccgccggcg acggccagct ggcgatcatg aacaccggtt
    38281 caccgaactt gctcgacggt atccatgccg atgggatcgc cattttccag gaggcgcgct
    38341 ggcacacggc cggcgtcgtc ccggacgcgt tgcatctgaa tccgctggct gcgtggctgc
    38401 gccggcgtat cgacgaggcc ccggacgcat tgcagacgtt tgccaccgag gctctgggtg
    38461 cgctccatcc gccggcggcc gatcttgcgg aggtggccag cggggtgctg gcgaccgtgg
    38521 tctcgcggca tcccgacagc ccgctcatcc tgtggtttcg cggtgaacag ctgcagacct
    38581 acaactgggc cgggaacccc ggcgaaaagc ccacggccat cgggccgctc gggccgcgcc
    38641 tcacgccgcg gcggagcttc gatctctggc aggaactggt gcgcggtcgc tcgacgccct
    38701 ggcgccgagt ggaggtcgaa gctgcccaac gattacggcg catggtgatg gatctggtcg
    38761 tcgtgcgcgc ggatcagttg gcggtggcca acgctgaact ggcaagcagc aacgccgaac
    38821 ttgatgcctt tgcgtatgtg gccggacacg atctgaagga gccgctgaga ggcatcaaca
    38881 agtacgcgca tcgcctgctg gaagacacca aggcgggacg aatccaggat accgaacggg
    38941 tcgagtggct gcttcgaatg accgtccgca tggacacact gctcaatgcg ctgctccact
    39001 tttcaagggt gggccggctg tctctggact gcgccgacat tgagctcgga ccggtccttg
    39061 tcgaggcgat ggacatgctg ggtgcacgcc tcgccgagag ccgggtcgag gtgcgggtgc
    39121 cgcgtccact tccgatggcg cattgcgatc gcatccgcgt gcgcgagatc ttcgccaacc
    39181 tcgtctcgaa cgcgacgaag tacaacgaca agccacatcc ctgggtcgag atcggatgga
    39241 tcggcgggca gggcgaagcg ctcacgatgc aacgaccggc atcgacgccc gcggacgcca
    39301 ggggcgacac cctgttctat gtgcgcgaca acggcatcgg catcgagacg cgtaacaagg
    39361 accgcatctt cgcgatcttc aagcgcctgc attcggctga tgccttcggc ggcggcagcg
    39421 gcgcgggcct gacgattgcc cgcaagatgg tgcagcagca cggtgggctg atctggttcg
    39481 actcgcaggt cggcgaaggc acgaccttct acttcacgct gcccgacccg gcttcctcca
    39541 gattgggcgt ccatggcgca tgaccgcgaa cgtgatgggc gtcccgtgct ggtggtcgaa
    39601 gactccgacg acgacttcga caccgtggtc gaagcggcca ctcgcgccgg agtgcgcaat
    39661 cggctgattc gtgccgcagg tgccgaaatc gcgcgaagcc tgctcgcgcg cgaggcagcc
    39721 ggctcgttct cgttcatgtt gctggactac aacctgccgg gcgtggacgg gctggccttt
    39781 cttgaagtcg tgcgccgcga cccggtgctc gctgcgctgc ctgcggtcat ctacaccacc
    39841 agcgtcaatc cgctggatcg cgacgccttc ttgcgggctg gtgcgaacgc gttccatctg
    39901 aagagcgtcc agcatgcgga cagcctgagg acgctggagt cgatcttcga tcattggctg
    39961 acgcgtgctg cacctgcgcc gggcgggcct cgcgcatgaa ccaccaagcc caggagccga
    40021 tgcgggtgct gatcgtcgat gacaacccgc tcgaccgggc cgacgccaag gcggcgttga
    40081 tccaagggtc cgggcgtgta taccagttca gtgaagcctc gtcggcagaa gaggcgctgc
    40141 atctgtgttc ccaatggccc cttccgcact gcatcgttct cgacctcggc ttgccggatg
    40201 ccgacgagtt cgacgtgatg atccggctgc cgcgcgatga agacaatctg atgcgcatcc
    40261 ccgtggtggt gctgacggcc tccgtggaaa tcggcctcag ccaggccgcc ctgcgcgctg
    40321 gcgcgcagga ctatgtcggc aaggcctggc tgctgcccga gaccttgacc caggcggtcg
    40381 aaaatgcgat cgaacgactt cgcatgacgc gctcgctcga agcgcaacgc cgcatggccg
    40441 acgccgcgcg acaacaggcg ctgcaactcg aggccgagaa ccgccagatc caggaagcca
    40501 accgactcaa gagccagttc ctggccaaca tgtcgcatga gttgcgcacg ccgctggccg
    40561 cagtcatcgg tttcgccgac ctgctgctga cggggcaggt gccgccggat tcgccgaagc
    40621 accgcctgtt cctgggccac atcggcacca gcgggcgcca cctcctgcgc cttgtcaacg
    40681 acgtgctgga tctgtccaag gtcgaatccg gcaagttcga gttctttccg gaatcgctcg
    40741 atctggctga ccttgtcaag ggagtcaatg acgttttggc cacggagttt caactcaagc
    40801 aacttcgggt tgcgaccgac atcgaggcga ccttgggcgg cctcgtgctc gatcctttgc
    40861 ggctcaggca ggtgctttac aactacctgt ccaacgcgat caagttcacg cctgtggccg
    40921 gcctgatcac cgtgcgggcg tatgccctga gtgcgcgcca cttccggctg gaggtcgagg
    40981 acaccggcgt cggcatcgcg gcgccggaca tggcacgcct gtttaccgcg tatcagcaac
    41041 tcgacgccgg gtccaccaag cgacatcagg ggaccgggct cgggctggcg ctcacgcgcc
    41101 gcctggtcat ggcacagggc ggcagtgtgg gagtccgcag cacccctggc aggggcagcg
    41161 tgttccacct ggttctcaac cggatccacg gagccgacgc cgcgcgcgag tcggaatatg
    41221 aggaacgtct cgacgcgtcg atggacgctc gtctgctggt gatgcacgac gggcatggca
    41281 acctgaacca tcgtgccggc catggcgggt tgccggtggc agtccgctcg cacttttcga
    41341 accttgacgg cgaggcgaac aagcggtgcg ctgaccgaaa aggaacgtga acgcgaagcc
    41401 ggcgagggaa ccatgcctgt ccgggagcgc agcttgaata tcggccttgc agaactgctg
    41461 aagaccgaaa cacgcgcggt gcacagcgcc gcggagcgca gcgtgttcct ggccgaattg
    41521 ctgagcgggc gaatggatcg gccggcgtac tgcgcattcc tgcgaaacct gcacgccatc
    41581 tacaccgtgc tggagcagtc gctcgaacga catgcgcacc atccgatgat cgtcccgatt
    41641 ttctcccggc gctgtggcgc acctgcgcac tcgagcacga cctgcgcgcc ctgcacggag
    41701 cagcctgggc tgacgagctg gcgctgcagc cctcggcggc gacctatgtc ggcaggctgc
    41761 gcgatctcga tgcggagcag cccggtctgc tggtggcgca tgcgtacgtg cgataccttg
    41821 gtgacctgag cggcggccag atgatgtgca gagtcgtcgc gaccaccacg gcgctggacg
    41881 gggcggtcgc gttctacgat ttcggcgacg ggtccgtcac acaggcgctg acgctggcat
    41941 ttcgacacgg cctgggcgcg ataccgatgg atcagtcccg cgccagcgca ttggcgggcg
    42001 aggccaggct ggccttcgac ctgcatcagc gtctcttcgg cgaactgtca gcggcttgcg
    42061 gactcactgc acgagtccgg gcactacccc gatggggcgg cgagggctga ggactgcgcg
    42121 gagcctggat cgacgatgtg aggcccagtt gaggaagcca gccgagtgct gctgagcccg
    42181 gtgggcccgg ttcataaacg ggcggtgctc agatgtcgcg gatcagttgc cggaactcgt
    42241 cgacgtcctc gaagctgcgg tacaccgagg cgaagcgcac gtaggccacc ttgtcgatgc
    42301 gcttgagttc gcgcatgacc agttcgccca gccgtgtcga gggcacttcc ttggcgccgc
    42361 tgttgagcag cttctcctgg atgcgctcga tcgccgcgtc gatctgctcg acgctgaccg
    42421 gccgcttgcg cagcgccagc gacatcgatg cacgcacctt gccgatgtcg aactcggcgc
    42481 gcgtgccgtc cttcttgacg acggccggca gcgcgatttc agcgcgttcg taggtggtga
    42541 accgcttgtc gcagctgtga caccgacggc ggcggcgcac cacgtcgcct tcgtcggatt
    42601 cacgcgtctc gacgacctgt gtgtcgacgt ggctgcagta cggacaacgc atggcagtgg
    42661 ggccgtggac gtcgtcggat caggtcggat cgagatcgcg cagcgggccg cggtcggtgg
    42721 gttgccggcg acgggctcag cggtaaaccg ggaagtcgcg cgtcagcgcg gccaccttgc
    42781 tgcgcaccgt cgccaggttc gcttcatcgt gcggctggtc cagcacgtcg gcgatcaggt
    42841 gcgcggtgag gcgcgtctgc tcttccttga acccgcgcgt cgtcaatgcc ggcgtgccca
    42901 ggcggatgcc gctggtgacc atcggcttct gcgggtcgtt cgggatgccg ttcttgttgc
    42961 aggtcatgtg ggccaggccc agcacggctt cggcttcctt gccggtgagg ttcttcggtc
    43021 gcaggtcgac cagcatcaca tggctctcgg tgcggccgct gacgatgcgc agaccgcgcg
    43081 agatcagcgt ctcggcaagc acccgcgcgt tggtcaccac ctgctgctgg tagaccttga
    43141 actccggcgc cagcgcttcc ttgaaggcaa ccgccttgcc ggcgatcaca tgcatcagcg
    43201 gtccgccctg caggccgggg aacacggcgc tgttgatctt cttggccatg tcctcgtggt
    43261 tggtcagcac gatgccgccg cgcggtccgc gcaggctctt gtgcgtggtc gaggtcacca
    43321 cgtcggcgtg cggcagcggg ttcgggtaga cgccggcagc gatcagcccg gcgtagtgcg
    43381 ccatgtcgac catgaagacc gcgcccacgg ccttggccac ccgggcgaag cgctcgaagt
    43441 cgatgcgcag cgcataggcc gacgcgcccg cgatgatcag cttcggcttg tgctcgtgag
    43501 ccaggcgctc catcgcgtcg tagtcgatct cttcctgcgc gttcaggccg tagctcacga
    43561 ccttgaacca cttgccgctc atgttcagcg gcatgccgtg ggtcaggtgg ccgccttcgg
    43621 cgaggctcat gcccatgatg gtgtcgcccg gctgaagcag tgcgaagaaa acggcctggt
    43681 tggcctgcga gccggagttc ggctgcacgt tggcgtagcc cgctccgaac agcgtcttga
    43741 gccggtcgat ggcgagctgc tcggccaggt cgacgaactc gcagccaccg tagtagcgct
    43801 tgccgggata gccctcggcg tacttgttcg tcaactggct gccctgtgcc tgcatcacgg
    43861 ccggcgaggt gtagttctcc gacgcgatca gttcgatgtg gtcttcctgg cgctggtttt
    43921 cgctctggac gaccgaccac agttcgggat cgatgtgtgc aagggtggat tgggtgcggt
    43981 caaacatggc gacgatcctc tacaacggga acacgggatt caggacgcga cgcagggtcg
    44041 catgacctgc ggcatgcagc agggccggcg gaccatctgt ccgctgtggc caggagccgg
    44101 attttcagcg tttcagcgca gagcagccag cttctcgtcg gcgggcggct cggaggattc
    44161 gctggccggc gccggagcct cggctgattc tgcctgcgct cgcgatgccg tcggcaccac
    44221 gttgggcgca ttgaccgcga cgagcttgcc gcccgccacc gcctgcaggt ggttttgctc
    44281 ggacaggacc ttcagtgcat atccgccgtc gtcgctcagg ttggcggcac ccacgtagga
    44341 gcgcaggccc gcctcgacgc caccggcgcg cgcaatgcat tccttgagca cctgcacacc
    44401 gacacgcagg ttggtgacgg gatcgaacgc ggcgaggttg ccgccgaact gctcgtactt
    44461 gtcgtcgtgc acgcgcgtca tcacctgcat cagaccctgc gcaccgacgg gactctgggc
    44521 aaatgggttg aagctcgact cgatcgccat gatcgccagg atcagcgtcg ggtccaggcc
    44581 gacccgtgcg cccacgcgcc aggcctcggt gaccagacgg ctgaccggct cgggcgccac
    44641 cttgtagcgc cgcgccagcc attgggctac ggcagcctgc tggcgggtga gatcccgcgg
    44701 gtcgctggcc gtggcgcgcg cgatcgcttc gggttcgcgc agatcgttgg ccatcagctc
    44761 gagcggatcg gtgcgggctt cctggcgcgc ctgcagccag ccgagcgcct gcatttccac
    44821 cgtgtgccgg agttccgagc gacccgccac gaagacgatc agcgcgaccg cgatcaggcc
    44881 gaccagtgcg agcgtgttgt ggctcacctc cagcaggccg tgcccgacgt cctgcatgaa
    44941 gacaccggtc gaacgcaccg tggcgcggca ggactgctgc gctttcaaag acaactgccg
    45001 gagtacggac atgactcttc tccctctccg ccccgaaccg ggcttgcgcc gcggggctga
    45061 ttcgagcgtc gcgccgaaca taatcggttg cacggacggg ctggacacgt ttgtgagacg
    45121 ggtacggacc gctgcgagag cgctcgaaat gaatctgatg aacgttgcct tgatctaggg
    45181 taagtaccca tcaaggacaa ccggacggga ttctaggaag caaaaacata accggtcaac
    45241 cattacggac cggttcataa ctacaaatga atatgaaata ccgcgatctc cgggagtttt
    45301 gtgcgggtct ggaggccgtg ggcgagctcc agcgtgtcgc tgcgccagtg tcggcgcggc
    45361 tcgagatgac cgcgctgagc gatcgggtgc tgcgtgccgg agggccggcg ctctggttcg
    45421 agcaaccact ctattccggg ggggggtaca aaattccggc cttgacaaac ctgttcggga
    45481 cgaccaaacg ggtggcgatg ggcatgggcg ctgccgacat cggtgatctg cgcgaggtgg
    45541 gtcgggtgct cgccagcctg aaggagcccg aaccgccgaa gggtctgaag gacacaggcc
    45601 ggttgctgca cctggtcaag tcactttggg acatgaagcc ggccacggtt cgccaggcgg
    45661 cgtgccagca ggtggtgatc gagggatcgg acatcgatct gctgggcctg ctgccggtgc
    45721 agacctgctg gccgggcgac gccgggccgc tgatcacctg gggcctggtc gtgacacgag
    45781 gcccccaggg cggctcgaac ccgcgccgcc ggcagaacct gggcatctac cgccagcagg
    45841 tgatcgcgag ccgtcaggtc atcatgcgct ggctggccca ccggggcggc gcgctcgatt
    45901 tccgcgattt cgcacgggcc aaccctggcc ggccgttccc tatcgtggtg gcgctcggcg
    45961 ccgatcccgc caccatcctc ggtgcggtga caccggtgcc cgacacgctg agcgaatacc
    46021 agttcgccgg ccttctgcgc ggcagccgca ccgaggtcac cgactcgggc gtcggcgatg
    46081 ccggggtgat gctgcaggtg ccggccagcg ccgagatcgt gctcgaaggg cacatcccgg
    46141 tcgcagcctc cggctacacg ggccgcagcg agcacggcgt gccgctgaag gaagtgggcg
    46201 gctatctata tgcactggaa ggtccgttcg gcgatcacac cggctactac aacgagcagg
    46261 actggttccc ggtgttcgag atcgaccggc tgacacatcg ccgcgacccg gtctatcact
    46321 ccacctacac cggcaagccg cccgacgagc ccgccatcct gggcgtggcg ctgaacgagg
    46381 tgttcgtgcc catcctgcag aagcagtttc cggagatcgt cgacttctat ctgccgcccg
    46441 agggttgcag ctaccgcatg gcgatcatct cgatcaagaa gagctacccc ggccatgcca
    46501 agcggctgat gttcggggtg tggagcttcc tgcggcagtt catgtacacc aagttcatcg
    46561 tggtgaccga tgacgacgtg gacatccgca actggcagga cgtgatctgg gccatcacca
    46621 cccgcatgga tccggttcgc gatgtgacgc tggtcgagaa cacgccgatc gactacctcg
    46681 acttcgcttc gccagtcagc ggactgggcg gcaagatggg gctcgacgcc accaacaaat
    46741 ggcccggcga gacgtcgcgc gagtggggcc gcacgatcgc gatggatgcg gcggtggaag
    46801 acaaggtggc cgacgtgttc gatcagatca tgcgggccgc gcgctgacga ggaacccggg
    46861 gccatgaaac ctgaaacagt gcttgtggtt cgccgccccg aggcgtaatc tgggtcggcc
    46921 tgcatgcagg tcatcccagc ggcgcagtcc ctgcgcccca ggggccccgg actttcaccc
    46981 tccggagccc cgaaccggtg gctccagcca cctgcccctc ccaccgtgat ggtcggaggg
    47041 gttcttgttt ctggggcagg gtgaccggca agccatctga ctactgtatg aaaaaacagt
    47101 atattggggc gtcatgtctg cgccccggct ccccttgatt cctgaaagcc tgcgctcggt
    47161 cctgtggcgc ggcgaccaga tggagcgctc ggtcgatccg gtcctgccca gcggttttgc
    47221 tctgctcgac gggcagttgc cgggtggtgg ctggccccgc cgtgtgctca ccgagctgct
    47281 gttgccacat cccggggtcg gcgagatccg tctgctcagc ccctgcctga tcgcgacgca
    47341 gcggcagggc cgcctgctga tgctgttcga tccgcctgcc gccttgtcgg cccaggcgct
    47401 gtcgcagctc ggtttcgatg tggagcaact gctggtcgtc cacacccggg cacgcgtcat
    47461 tcccggctcc gacagcctgt gggcgttcga gcaggccttg aaaagcgggc atgtcggtgc
    47521 gctgctggcc tggctgcctc cccggttgcg ggccgatcgc ctgcgccggc tgcaactggc
    47581 tgcccaggcc cacgacgggc cggcgttcgt gtttcgcgaa ctggccgcac aggagcgtcc
    47641 cacctcggcg ccgctgcggc tggcgctgcg cccgggtgga gccgacatgc tgcgggttcg
    47701 cgtgctcaag cggcgcgggc cgccgctgga ggctccgctg atgctggcct tgccgcccgt
    47761 gctgtcggat gcgcttcgag ccgcggcatg gcagcgacag cagccagcgg gcgacgagcg
    47821 cattccagtt caggaacaca gggcccgcaa gcatcccgcc ggcagtcatt ccgccagtgc
    47881 gcccgctgcg gctgtgccac tgcccgccgc caccttcatc agcctggtct gatcggcgcg
    47941 cctcatgttg tggatcgcgc tgcatctgcc gcgcctgtcg ttcgagtcgt ggctggccct
    48001 gctaccgccc gatcagcgtg agcagcctgc agccttgatg ggcgcccggg acgtgctcct
    48061 gtccaacgcg tcggcacatc aactgggtgt gcgacccggg ctcagccggg ccactgcgct
    48121 ggcgctggcg ccgcacctgc ggatggggcg ggccgatgcc gtgcgagatc tgcaggccct
    48181 caccagcgtg gcgcatgtgg cgttggcgtt cacaccgtcg gtctgctttg caaccacgcc
    48241 gatgcttgct gccgcgggtt gcgtgggtca tgccggagcc gagccgggtg gtgcgctcca
    48301 caccgtgatg cttcaggtgc agtccagcct gcgctgtttc ggcggacagc aggccctgat
    48361 gcagcggctg cgtgccgcat ggctgccgct cggatgcacc gtgcagctcg ccagtgcgcc
    48421 caccgcgcag ggcgccgccg tgctggcacg gctggccgag ggcgacctcg gcgagctgca
    48481 ctgtgccgac ttgcgctcca tggcgtgtcg gctcgatggg gctccggtgc agttgctcgg
    48541 actgggccgc gatatcttgg acgtgctcga aggcatgggt ctcggaacgc tcggtgcgct
    48601 gcgccgcttg ccgggccgcg gtctggcgcg tcgcttcggc gagggcttgc tcgatgccct
    48661 ggcgcgcgtg tacggcgagc gccccgatcc gtccccggcc tggatcacgc tgcccgatgt
    48721 cttcgaggtg cgcatcgagt tgcaggcccg cgccgacacc accgggcagg tgctgcacgg
    48781 cgcgcagctg ctcatcgaac cgctgctggt ctgggcgcgt gcgcaccagg cccgggtgcg
    48841 gcgtttcacg ctgttcatgc accacgagcc gagacaccgg aaggacgccg gcacgccctc
    48901 ggtcaccgaa ctgacgatcg cgttggccga accgtcgtgc gatgcggacc acctgtcggt
    48961 gctgctgcgc gagcgtctgg cgcacctgtc actgcctgcc cccacgctgg aactgcgact
    49021 gcactgcgcc gacgtggccc gaggctcgcc gttcagcgcc gagctgttcg cttcggcgcg
    49081 cggcgaacgc gaggggctgg ctcgcctgat cgaacggctg caggcccgtc tgggcccgca
    49141 gcaggtgcag cgcctggcgc cagcggccga tcaccgcact gtgcgccacg gcgtgcctgg
    49201 acgcagcgtc gctcaagccg cgtccgcacc gcgtccggtc tggctgttgc atgagccaca
    49261 gcccttgccg gagcgtcaat cgcgcccctg gctcgatggt cagcccctgc aattgctgtg
    49321 cggcccggag cgcgtcgagt cgggttggtg gaccgtcccg cgcaccgtgc gcgactactt
    49381 cattgcccag acgcccgaag gtgccctggt gtggatctgc cgtgcccgcc tgccgctttc
    49441 ggtcgaggag tctgcttcag gctggctggt ctgtgggtgg tttgcctgag gcgatccggg
    49501 gtgggcttgt aggaggaagc gcacacgcgc gtgtcgcggc ttctgggtga caccccgatc
    49561 gggcctgtct acagtgagtc ggtccgtcac acggtcatcg gcgatgcatc gatggcttcc
    49621 atctcaggag aacctcatga ccgcattcaa agccgcttgg tatccgctga ttgcggcaac
    49681 cgccattgtc ctcgcggcgt gtcagccggc cgagggaccg gtcgaacgcg ccggcaaggc
    49741 cgttgaccag gccgcccaga aggcgggcga ccagatcgag aaggccggcg acaaggtcaa
    49801 ggatgcggtc gaggacgcga agaaatagtc cgcttctgca gcgtggcatg ccggcgccca
    49861 cgcgccgggg ccgcaggggg tgaatcaggc ggcttgctcc agcggggaac ccgtgtgcat
    49921 ctttcgctag agtgcggtgt gtcccctgcc gcctgcgcgc acgatccatg ctgttccttc
    49981 tgtcccccgc caagacgctc gactacgaca ccccttcgca cgtagaccgc ccgacgacgc
    50041 cgcagttcgt gccgcagtcg gccgagctga tcgacatact caggaccagg agcgtggccg
    50101 acatcgccac gctgatggat ctgtcggatc cgctcgctgc attgaacgtc gggcgctatg
    50161 gcgtgtggag cacccgcttc accaagaaga acagcaagcc tgccgtgctc gccttcaacg
    50221 gcgatgtgta cgaaggcctc gatgcagcgt cgctgagcgc cgacgatctc gactgggcgc
    50281 agcagcacct gctgatcctg tcgggtctgt acggcctgct gcgccctctc gaccggatgc
    50341 agccctaccg cctcgagatg ggcaccgcgc tcgagaacgc gcgaggccat aacctgtatg
    50401 catactgggg cgacaccctg gctcgccaca tcaaccagcg gctgaaggcc gacccgtcgc
    50461 cggtgatcgt gaacctggcg tcgcaggagt acttcaaggc cgtgcagccc acgcagaaga
    50521 gcgcctggct gaagccgcgc gtcgtcgagt gcgtgttcga ggactggaag ggcggccagt
    50581 acaaggtgat cagcttcttt gccaagcgtg cgcgcggcct gatggcgcgt tacgcgatcc
    50641 agcaccggtt gctgacgccg cagggcctgc agtccttcga cctcgaaggc tatgcctgcg
    50701 atgccgccgc gtccgagccg catcggctcg tcttccgccg caggctctga gcgccagcca
    50761 tgccggtgcc ccagcggatc acgcctgaac tgcagcgctg gatcgtcgat caggcaacct
    50821 ccggtcagtc gccagatgcg gtactgaggg cgatgcgcga cagcggctgg tccgcagccg
    50881 tggccgagca ggcgctcgaa gacacattgc gcgatcacct tgccgatcgc agccaggcgc
    50941 tggccctgcc gccgaagctt cgcgtgcccg aaccgctgtg cagcgatggc agcctgtcga
    51001 tcgatctgac cgaagaagct tcggccgaag tgcgccaggt gcgggtcctg agcagcctga
    51061 cgaacccgcg catcgtgctc ttcggcggcc tgttgtcggc cgccgagtgt gatgaactgg
    51121 tggcgcttgc gggtgcgcgt ctcgcgcgct ccgagaccgt cgaatcgagc accggaggag
    51181 gcgagatcaa cgagtcacgc accagcgacg gcatgttctt cgggcgcgag gagaacccgc
    51241 tgtgtgcacg catcgaaaga cgcatcgccg aattgctgcg ctggccgctc gagcgcgggg
    51301 agggactgca ggtgctgcgc taccggcccg gtgccgaata caagccccac tacgattact
    51361 tcgacctgga gcaggcgggt acaccggccc tgctgacccg cggtggccag cgcgtcgcaa
    51421 gtctggtcat gtacctcaac acgcctgcgc gcggcggggc gacgatcttt cccgacgtcg
    51481 agttcgaagt cgcgccggtg aagggcaacg cggtgttctt cagctacgac cggccgcacc
    51541 cggtgacgcg atcgctgcac ggcggcgccc cggtgctcga aggcgagaag tgggtggcga
    51601 cgaagtggct gcgggagggg cgattcgagt gatcacgtgc gcgccgcgcg tctcaggcgg
    51661 cgtcgacgct cgagaacggc tggctgcgtt cgatgcggcg ccatttgcgc tgctcccagg
    51721 tctgcggtcc ggtcaacggc cagccctgca tcgccggcgc cagttcggcg cgtggcacgc
    51781 gcatgaatcc cgaggacagc gtgagcccgc gcagctgttg ccaccagtgg gtgcggcgca
    51841 cgctggacgg cagcggcgcg cttgaatgcg gcgtccagta gatgcacgcc cgctcggtcc
    51901 agtacgcctg acgctcgaaa ttgacgcggc cggcaaagcc ggtatccacc ggaaacagca
    51961 gcccgtcgtc ggccagttcc accacggtga acaccaggtc ggtcggcagg tcgccgcgtc
    52021 cccggtgcac gaacacgatg cgttcgactt cgtcggcggc gaagcgatca acttcgacat
    52081 cgtcgcggaa cacggcgatg tccttgcctt gccagagcgt gtgccatctg atggccatgc
    52141 gcgaacccct cttgtcgttt gcgagaacaa aagagggtag gcccgtcgcg gccgttcggg
    52201 ccgtgacaga gcagctcagt tgcaagtcct tgtgtccggg ggggcacgcc tcccggcgtg
    52261 ccggggggtc agcgcttggg cgtcgtcgaa cgtggcgcga acatcgcgcc gccttcgcgt
    52321 ctcggctcgg gccgcgggcc ggcaggccgc gcagccggcg cagcgcggtt gccctgcggt
    52381 gcatttccat cgcgtgatgg agcacgcggt gcctgaccgc caccgcccgc acccgatcgt
    52441 gccggagctc caccgccgcc tgcgcgagcc ggcgcggacc ggccgtcgtt gcgaccacgg
    52501 ccgccgccac caccaccacc gccgccgccg ccgccgccgc tgcgaccacc gccggagcgg
    52561 aagccgccgc ggccggcgcc ttcaccgatc accatgcgac ccagcacgat cggctcggcc
    52621 ttctcgccca ccggaggcgc gaagccttcg acgatctcct tcgggatctc gcgcttgatc
    52681 agccgctcga tgtcgcgcag gaagccgttc tcgtcggggc acaccagcga gatcgcctcg
    52741 ccctgcgcgc cggcacggcc ggtgcggccg atgcggtgca cgtagtcctc gggcacgttg
    52801 ggcaactcgt agttgacgac gtgcggcagc atgtcgatgt cgatgccgcg tgcggcgatg
    52861 tcggtcgcca ccagcacggt caggtcgcct gtcttgaatt cggccagcgc cttggtgcgc
    52921 gcgccctggc tcttgttgcc gtggatggcc atggcgctga tgccctgctt ctccaggaac
    52981 tcgaccagct tgttggcgcc gctcttcatc cgtgtgaaga ccagcacctg gtcccaatcg
    53041 ttggacttga tcaggtgcgc cagcagatcc ttcttcatct cgcggccgac cgggtgcacc
    53101 ttctgcgcga tcgtctcggc ggtctggttg cggcgggcca cttcgatcag cgccggcttg
    53161 ttcagcaggc ggtcggccag ggccttgatc tcgtcgctga aggtggcgct gaacagcagg
    53221 ctctgtttct gcgcgggcac gatggccagc accttcttga tgtcgtggat gaagcccatg
    53281 tccagcatgc ggtcggcttc gtcgagcacg aagatctcga cgtggctcag gtccagcgtg
    53341 cgttgctggt ggtggtcgag caggcggccg ggcgtggcca cgaggatgtc gaccccgcgg
    53401 cgcagcttgt cgatctgcgg ctgcatgccg acgccgccga acatcaccat cgaggtgagc
    53461 ttgctgtact tgccgtaggt gcgcacgctt tcctcgacct gggccgcgag ttcacgggtg
    53521 ggggtcagga tcagcgcgcg gatgggcagc ttgcccttgg catcgcgcac cgcgggcttt
    53581 tccatcaggc ggtgcagcat cggcagcgtg aagccggcgg tcttgccggt accggtctgg
    53641 gcgcctccca tcaggtcgcc gccggcgagc acgacgggga tcgcctgcat ctggatgggg
    53701 gtgggcgtgg tgtagccgta ctcggcgacc gcacgcaaca agggctcggc gaggccgaga
    53761 gattcaaaag acatggagat tccaaacagc ggatcggcct gtcgctcgaa cttcgagcgc
    53821 cagtcgcagg cagaggggtg tgaggggcgc caacgatggg gcggcccggt caggatgaca
    53881 tcagcgcagt gactgggtgc tgcgttggtt acaggtagtc taacgggttt tcccgaataa
    53941 gcgggttgat caacgggatg aagccggccc tgttgtcgct gagggcggcc cggggccatg
    54001 atcccggaca tgacggcccc gcagaacctt tgcccgctgc tctggcctcg ccgtgccatg
    54061 gtgatgcagc tgctgggctc gtgtgccgcc tggcagggcc tcgcgcatgc tcggcccgct
    54121 tcccggtcgg cgaacccggg cgacctcgat ccgtcgctcg atgccgtcat cgccggaacg
    54181 cagcgcagtg cagaccactc tgcgcgggac cgctggcgac atcctgccga gaccctgcgc
    54241 ttcttcggcc tgcgtgccga ccagcaggtg cttgaactgt cacccggtgc aggctggtac
    54301 accgagatac tcgcgcccta cctgcgcgag cgtgggaagc tttacgtggc gcactacgca
    54361 tcggatccga gtcggtctga caccgactac gagccgcgga cccgcaaggc cttcgaggcg
    54421 aaactggccg ccaccccgac cctgtacgac cgagtcgtcg tcggcacgct gccgacgacg
    54481 gccttcaccg acctccactt cgatcgaccg ctcgatgctg tactgacctt ccgcaacctc
    54541 cacaactggg tccgggacgg ccgcctgctg caacggctgc ggatctttca tgcggcgctg
    54601 aaacccggcg gcgtgtttgg cgtcgaggag caccgcgcag caccggacac gccgctggac
    54661 cgtcagatca gcagtggcta cctgaccgaa gcgctggtga tcgggcacgc aaggtctgcc
    54721 ggtttcgaac tgcacgggcg cagcgagatc aacgccaacc cccgcgacag ccggctccat
    54781 cctcacggcg tgtggtcgct gccgccgaac ctgagcggcg cccccgaagg ctcgaaggcc
    54841 cggatggagg cgctggccac cggagagtcg gaccggatga cgctgcgctt catcaaggcc
    54901 cggacctgac gccccgtgca gccgccggcc cgggttgcag gcgagcgacg acaatagcgg
    54961 ccattcgcag tccgctccgg gcggaatgcc cctggctcaa ggcaaggcag ggtctggcac
    55021 gtttcgcgag aagcgcctgc cccctgatgt cgctctctca ttcgtcgcat ccactcagtt
    55081 caaggcaaac catggcgcaa cacgttcttt acaagcaact gcccgccttc ggcggtcttc
    55141 actcgctttg ctccgggagg gccctgtaat ggcgcagtac gttttcacca tgaaccgcgt
    55201 cggcaagatc gtcccgccga agcgtcagat cctgaagaac atttcgctca gcttcttccc
    55261 gggcgccaag atcggcatgc tggggctgaa cggctcgggc aagtcgaccc tgctgaagat
    55321 catggccggc gtcgacaagg acatcgaggg cgaagccacc ccgatgcccg gcctgaagat
    55381 cggatacctg ccgcaggaac cggtgatgaa ccccgaccag accgtgcgcg agtcggtcga
    55441 ggagggcatc ggtggcgtgc tggctgccaa gaagcggctc gacgaggttt atgccgagta
    55501 cgccgcggag gatgccgatt tcgacgcgct ggccgccgag caggccgaac tggaggcgat
    55561 catctcgtcg gctggctccg agaacaccga cctgcagctc gaactggccg ccgacgcgct
    55621 gcgcctgccg ccgtgggatg cggtgatcgg caagctgtcg ggtggcgaga agcgccgcgt
    55681 cgcgctgtgc cggctgctgc tgtccaagcc cgacatgctg ctgctcgacg aacccaccaa
    55741 ccacctcgac gccgaatcgg tcgactggct cgagcagttc ctgcaccgct acgcgggcac
    55801 tgtggtcgcg atcacccacg atcgttactt cctcgacaac gccgccgagt ggatcctcga
    55861 actcgaccgc ggccgcggca tcccctacca gggcaactac agcatctggc tggaccagaa
    55921 agaaaagcgc ctcgagctcg agcagaagac cgaggacgca cgcacccgcg cgatgaagcg
    55981 cgagctcgag tgggtgcgca agaacccgaa gggccggcag gcgaagagca aggcgcgcct
    56041 ggcgcgcttc gaggagctgt ccgacgtcga ctaccagaag cgcaacgaga ccaacgagat
    56101 cttcatcccg gtggccgacc gcctcggcca cgaggtgatc gagttcgaga acgtcaccaa
    56161 gagcttcggc gaccgcgtgc tgatcgacga cctgagcttc aaggtgcccg cgggcgccat
    56221 cgtcggcatc atcggcccga acggcgccgg caagtcgacg ctgttccgga tgatcgcggg
    56281 caaggagaag cccgacagcg gcaacgtgaa gatcggtgcc accgtgaaga tggccttcgt
    56341 cgaccagagc cgcgacagcc tggaggcgga caagacggtc tggcaggatg tgtcgggcgg
    56401 tctcgacaac ctgatgatcg gcaagttcgt cgtgccttct cgcgcgtatc tcggccgctt
    56461 caacttcaag ggcaacgatc agcagaagct ggtgggcaac ctgtcgggcg gtgagcgcgg
    56521 ccggctgcac ctggccaaga cgctgatcgc tggcggcaac gtgctgatgc tcgacgaacc
    56581 gtcgaacgac ctcgacgtcg aaacgctgcg tgcactcgaa gacgcgctgc tcgagttcgc
    56641 cggcagcatc atggtgatca gccacgaccg ctggttcctg gaccgcatcg ccacgcacat
    56701 cctcgcggcc gagggcgatt cgaaatgggt gttcttcgac ggcaactacc aggagtacga
    56761 agccgacaag aagaagcgcc tcggcgagga gggcgcccgt ccgaagcggg tgcgcttcaa
    56821 ggccctgaag tgaactgaac ttcggcctct gttgaaactt gcgccgggcg gcacgactcc
    56881 atcgtcgggc cgcccgtcct acttctgccg catcaacgca tgaccaatca ttccccagcc
    56941 ccgagcctgt ccttccgcgc acagtcctca tcgtccggcg cgatctccct ggccctgcgt
    57001 cggccggtgg tgccgaattt cctggcgccc gcgtgcgtgc tgctgctggc gggctgtgct
    57061 tcgctgaccc cgcctgagtc gacccccacc gctgcggcag ctgcgacggc tgcggtcgct
    57121 gctccggcgg ctgcctcgat gcccggcacc tcgcaggccg gtgtggtcgc caagctgccg
    57181 gcacccggag cccagatgcc cgcggccggc tccgcgcctg ccagtgcgcc agcaccgggt
    57241 gcccctcgtc ctttcgccga ggtgatcaag gacgccaagc agatgagcgg cctgttcacg
    57301 ctctggcaga aggacgacaa ggtctggctc gaactggccc ccggggattt caaccagccg
    57361 ttgttcctgt ctcccaagta caagaccggc ctcggcgaag gccgctactt cggcggtctg
    57421 atgggcgaag aggtggtgat cgagttccgc cgcgtccaca accaggtcca gatgctggcg
    57481 cgcaacaccg aattcgtcgc cacgccgaag acacccaacg gccgtgcggt cgaggctgcg
    57541 ttctcgccca gcctgctggc atccaccacg gtggtgagcc agccgcaccc ggaacgcaag
    57601 tcggtgctgg tggaagcgaa cccgctgttc atcaacgaca tgatgggtgt cgcgatgggc
    57661 ctgcagcgcc tttaccgcca gggctatggc ctcgatgcgc gcaactcggc gatcaccgac
    57721 atccgcagcg gcccggacct ggtggtgctg gaggtgctga accactacgc caccggatcg
    57781 atcgccgtgg cgactccggg cgcaccggtg ccaccgggaa ccccgatgcc ttcggtgccg
    57841 cgttcggtgc ccgatccgcg cagcctgttc atgacgctgc actattcgct ggcgaagatg
    57901 cccgagcagg tgatggcgcc acgcaaggcc gacggccgcg tcggccactt caccagcaac
    57961 ctgtccgact tcagcgacga cctcgcccgc tcgccccgcc agcgcttcgt caaccgctgg
    58021 cggctcgaca agaaggaccc cgccgccgcg ctgtccgagc cggtcaagcc gatcaccttc
    58081 tggctcgaca gcacgatccc cgacaagtac cgcgccgcgg tcaccgccgg catcctggaa
    58141 tggaacaagg cgttcgagaa gatcggtttc aaggacgcga tccgtgtcga ggtgcaaccc
    58201 gacgacgcga cctgggacac actggacttc ggtcgcgcat cggtgcgctg gatgaccaat
    58261 tcgagcccgt cgttcggtgc catcgggccg agccatgtcg acccgcgttc gggcgagatc
    58321 ctcgacgcgg acatcggcat cgagagcctg tcgtcgcgca gcctgcggtc gctgcgatcg
    58381 caggtgctcg cgtcgtcccc ctcaacggac ctgcctgccg agctggccgc gcgcggcatg
    58441 cgatcgcaga gggcctgcga ctacgccgac caggcggccg agcagctcgg ctacgcactc
    58501 gacgtgctcg aagcgcgcgg cgacatcgac ccgagcagcc ccgaggccga gcagttcgtg
    58561 ctgggctaca tgaaggacgt gacgatgcac gaggtcggcc acacgctcgg cctgcgtcac
    58621 aacttccggt cgtcccgcgt ctactccgaa gagcagttga ccgacccggc gttcaccgcc
    58681 aaccacagca tcgccggctc ggtgatggag tacgcggcca tcaacctgtc ggcgcccggc
    58741 gtgccgacca gccgccaggg cacgccgttc aacagcaccc tcggaccgta tgactactgg
    58801 gccatcgagt acgcctaccg tccgttgccg gcagcccagg aagaagccga actcgcgaag
    58861 atcgcggcgc gcagcgccga gcccgaactc gccttcggca ccgacgagga caacttcctc
    58921 ggcatcgatc ccgagaccct tcagttcgac ctcggctcgg acgtgctcgc gttcgcgcgc
    58981 aagcgcatcg cgatcgcgcg cgaccttctt gagcggcagg aaacgcgcgt gctgtctccg
    59041 gaccaggatt actcgatcct gcgccggtcg gtcacctatg cgctgcgcga catgggccgg
    59101 gctgcgagcg tgctgacccg tcagatcggc ggcgtgcgca cggtgcgtga ccatgcgggc
    59161 agtggccgtg atccgctggc gccgctgccg gctgccgacc agcgcgccgc gctcgacctg
    59221 ttggcgcgcg gcttcctgtc ggccgacagc ctgcgcatct cgccggccct gcagcggaag
    59281 ctggctgtcg actacctcga acggggcgac gccgccctgc gcggtgacgg cacgccggcc
    59341 accgcgctgg tcaccgattt ctcgctggcc tcgcaggtca tcgaactgca gcgtagcgtg
    59401 ctcggccagt tgctgagcga cggcgtggcc gcccggctgc tcgacagcga ggacaagctg
    59461 ccgaaggacg cgctgcgcct gtccgagctc tatcggcggt tgaacgaaac ggtgtgggcc
    59521 gagctggggc agtcgggcga catcccgtcg ctgcgccgcg aactgcagcg cgatcacatc
    59581 aaccgggtcg cgacgcagct gctgcgcccc gccgcatcga gccgcgtcga cgcacgcagc
    59641 ctcgcgcggg cgcaggcaca ggtgctgctc acccgattga acacggccgc caggcgatcc
    59701 ggcctgagcg ccgaggccca ggcccacctg caggacagca tcgacacgct gcagcaggcg
    59761 ctgacggcca agctggcgcg cacgggcgcc tgacgttgat cggttgatcg cggtccccgc
    59821 cggcacctgc gggcggggag cgctgtcgct cagagggcga gcagggccgc gtcaatggcg
    59881 ccgagcgcgg cgagggctgg cgcgggcagg gcgatgtcca tgcgtcgcgg cactgccgac
    59941 tcgcgcggat tgatccgcac caggacggcc ttgcgatcga tcaatgccga ttggctgaaa
    60001 tcgcgcaccg acggaatcgc cgtgccggcg cccagttcga tcacgacggt ccgcgaggcg
    60061 ccgtccagcc agtgattcag cgacgactcc tgagccgatg agcggtgttc gatccagccg
    60121 gagtcgccga acatcaggat gttgggccgg gcgatcgagc cgcagtgcgg acagcgcggc
    60181 ggctcgttca acagcgtgca ggtgtcggtg tcgacgatcg gcacgaacgc atcggccggc
    60241 cagatgtcgt cgctgcacgg ttggctgcac tgcaggtggt ggatcgaccc gtggcactcc
    60301 gcgagcgcgt tcgcatcgaa gccggcgtgc ctgaagtgac catcgacgtt ggacgtgaac
    60361 acgccgcagc cgtggcgcct gccttctccc cagcgccgaa ggatgtcgaa gccggcatgg
    60421 ggcgcaatac gtcgatacag gcccaggcgg tgcccataga acccccaggc cagcgcgggg
    60481 tcggcttcga aggtgtgcgg gctcgccacc tgatggaact gcaggccgcg ccgacccagc
    60541 gccgggtagg ctcgccagaa gccctcggcc ccgcggaagt cgggcaatcc gctgtcgacg
    60601 cccatgccgg cacctgcggt gatcagcagc gcgtctgcgt cggcgatcag ctcggctgcg
    60661 gcagatatgg cagtggcttc ggcttccatg tcggctactc gcgggaccgc tggtcgatca
    60721 ggtcacgtga tcgctgcggc cagccatggg gagtggctgg cgatgaatcc catgacattg
    60781 agcgcgaccg tgccccagta agtcgatcgg aagcccggct tcgatgactt gtgccgcagc
    60841 agttgctgcg ccatcaatgc accgggccag ccgcaggcca gactgaagag gtgcagcgtg
    60901 gactcggggg tccgccagcg gccctgcagg gctgccgact tgtctagcgc gtaggccatg
    60961 aatgcgagca ggctggcgag caggtaggcc acggcgacca cgggcttgac cgaccattgg
    61021 gaagcgacga aggcgtacag caacacgaac gccgggatcg ccaggacgcg cgcgggagtc
    61081 cagggtgccg gcgactcgga gcgtgtacgg cgtcccggac tgcgcgtgcg aatgaactcg
    61141 acggaccggg cccgcttctt cccctgcggg cccagctcga cctcgaacga cagtggcagg
    61201 ccgacgaccg ggcgacccgt gccgggcgga aatgccttga tgtgcacgaa gatctcgtcg
    61261 ccgccttgcg tgggctcgat gaagccgaag cctcgttcat cgttccagga tctgagcttt
    61321 ccgtcgaagc gcatggtgtt cggtgaggtg gcgggccgtt ttatcagaag ggtctcggat
    61381 gtaagctgct gctggcgcgc gagcaagtcg cgagccctga tgacccgaac cgggtcatgg
    61441 caattccggg cagtctccgc gaattcgcgg gcgattcaat gcaggtcttc tttcacatcc
    61501 gccgtaagaa ttaaagaatg ctttgttttt ggtggttcat gaaaagccgc cttcacacct
    61561 tgcgcacctg ctacgggttg cccagctcgg cgaaggcacc cgcgatcgcg ttgttgtact
    61621 tgagcttgag cagcggtgac agcttctcgg cgtccagttc gtcgagaccc tgcctgacgt
    61681 acttcgccag cacgaagtcg acgaacgcct gctgcttgtc tgtgaactcg gcggccgtgg
    61741 ctgacttggc tgccacagcg cgctcggccc gcgtcaccgg ttcggctgcg aaggccactt
    61801 ggaccagcac gtcgaacagg tcgtgttctc ggtttcgatg atcttctgca tctgggccag
    61861 cggctcgcgg ccgacgacct tgtcggccag cagggccttg caggaggcag cccggacgct
    61921 gcgatccagt gtgccgacaa ggcgctgtaa caggccaaga gagaagggcg caaccgcgtg
    61981 gtctttgaga cgcccctcgc cgatggcagc cggcctgggt tgccggccct ggcgggtgtg
    62041 cctgaccacg gcagccggcg ttacagccag gacggttgtc agcgatgaag ccagcgggcg
    62101 tgcatccagc gggctgccac atcgagggcg tagccgagtg cgccaatcag cacgatggcg
    62161 gccatcagtt cgtcgtaggc cagccgatcg cgcgtgtcga gaatgaaata accgagtccg
    62221 gccgacacgc ccagcatctc ggcgggcacc agcacgatcc agatgatgcc gatggccaga
    62281 cgcacgccgg tcaggatctg cgcggtgatg ccgggcagca ccacatgcag cacggtctcg
    62341 cgccgtgtcg cggacagact gcgcgacagc agcagccagt tggggtcgag ctgggcgaca
    62401 cccgcggcgg tgttcagcaa catcggccac accgcggcga acgccagcag gaagtacacc
    62461 ggggcatcac cgaccccgag caccatcacc gcaatcggca tccacgacag cggcgagacc
    62521 atgcgcagga actggaacac gggtgtggtg ccctcggcga agcgcttcga caacccggtc
    62581 agcacgccga gcggaacgcc gatggccacc gccaggccga gccccaccgc cacccgctgc
    62641 aggctggtga tcacgtgcgg cagcaactca ccgctggaca gcaaacgcgc cagtgcctgc
    62701 aacgagggcc cgggcgcaaa agacgcagcg atcggcgtgc gcaggatcag cagttgcgca
    62761 ccgatccacc acacgcccat cgccagcgcg agtcccagcc acggcagcag gcgacgccag
    62821 tgcgttacgg ccggcgccgc cgatgcgtcg atgtcagccg gcgcctcctt tgccagctcg
    62881 ctcacgggat gcgcctcaaa cctgcaccat ttccttgcgc agcagatcgg ccggcaggcc
    62941 gaacacctgc ggaccgccga cggcggcaag tgccttgcga acaaagcggt cgtcgaccag
    63001 atcgcctgcg acgaacttcg gattgagcgc ttccaggaag cgcgtgtcgc cctcgacctt
    63061 ggtctgccgg atggcggcaa ccagttcttc ggtgtagctc gcgaacggat atggctggaa
    63121 gtcgatgcgt cgctgcgccc agcccttgtg gcgcaccacg ccgctcgcct cgtagctggc
    63181 gtagtcggtc gcagcaagca ccttggtcag tgttcgcagc gggtgcggcg tgtagcggcc
    63241 ttcgcccgtg ctcgacagca tccgcgcggt gtcgacctgg ttttcgcgcg tccacaactg
    63301 cgccttcacg agcgcggtgg tgacgcgctg ggcccattcg ggacgctcga ccagatcgcg
    63361 ctcggccaga accgtggcgc agcaggcgtg gttcttccag acgtcacccg aaaaacggag
    63421 caccttgccc acgccggcga tctcggccgc agcgttgaag gggtcggcca cgatgaagcc
    63481 ggcgatcgag ccggctgcca gcgccgacac catctcggcc ggcggcatca cgatcaggtt
    63541 gacctcgttg ggcgcgagct gaccatcccg cgatcgcgtc accgccacca gattgctggc
    63601 gcgcaggatc tgctggagca gcacgttgtg gatcgaatac cagaacggaa tggccaccgt
    63661 gcgcccgccc agatcggccg cactgttgat gctcggcgcg accgtcagtg ccgagccgtt
    63721 gacgtggttc caggcgacga tcttggccgg aaacttcgat ccgtagcgca cccacagggc
    63781 ggccggagtc aggaggtgga tcacgttgac ctgccccgaa acgaaggcct cgacgatctg
    63841 cgcccagctg cgaaacagcc gcggcgcttc ggctcgcagg ccttcggctt catacatctt
    63901 gcggccatgc gccaccagca gcggagtcgc gtcggtgatc ggcaggtagc cgatcttgac
    63961 cggctggtca tcccccttga aagcctgggc acgcgcatcg ccggcgctga gcaacggcgc
    64021 tgccaccgag gcagtggcga gcgcgccgag gcgcagcatg tcgcgccgcg tcaggccgca
    64081 accgcaatcg gacgaggcgc agatgtgggt gtagcgcgag gcaagggagg gggcttcgga
    64141 cgtcatgggg tgttccttcg agtcgaggag gttcaggcgt ggcgcagcga ctgcaacgcg
    64201 gccaggatat cgagccgcag cgcagtcact tcggccgcgt gactttcgcg ggggcgttcg
    64261 atcgacacgg tccattcatg cagcacgcgc gctggttgcg cgggctcggc gcgccccatc
    64321 agcaggatgc ggtcggccac caggatggcc tcgtcgatgt cgtgcgtgac cagcagcgcg
    64381 gcggtgtgcc agcggtgtac cagttcgacc aacagcgtct gcatctcggc gcgggtgatc
    64441 gcgtcaagcg cagagaacgg ctcgtcggcc agcagcaatt gcggctcgcg cgccagcgcg
    64501 cgggccagcg acacgcgctg agccatgcca cccgacagct gcgccgggta gagcttgtcg
    64561 tggcgggaca acgagaccgc ctccagcgca tcgcgcacac gcttgtcgag cgccacgcga
    64621 tcgatctgcg gctgatggga gaaatcgagg ccgaaaccga cattgcccgc cacgctcaac
    64681 cagggcagca agctcggctg ctggaagacc agcgccgccc gcgggtgcgg cgccagcagc
    64741 ggcgcgccca gaaactcgat ctccccgcgg ctcggctccg ccagcttggc gagcacgcgc
    64801 agcagcgacg acttgccgca tccgctgccg ccgagcagcg cgacgatctc gcgtggtcgc
    64861 acgtcgagcg acaccgactc gaagatcggc tggcccttgc cgtaggaaaa cgcaagaccg
    64921 cggccactca gcgcataggg cgcggctgac gagtccgcag gctccgcgtt catcacagcg
    64981 ggcgcccggc tgttcgggcc gtcagtggtg tgcagggagg gcgaatcatc gaggttggag
    65041 gtagacaaag cccgccattg tttcatcctt ggcgcggacg tgttgaagcc aaggcgagtc
    65101 aacgcaagac tgtggcgccc cgcagcgcca cacgcaacga acgtctgcaa ctgtcgatgg
    65161 ccgaaatctg tccgaggccg aacatcggac ccgatgaccc gacgcaggcg gcaaaggcct
    65221 tgcgtcagcc gcataaaatc tcgctttcgc gaaatacggt ctcccatgca cggcctggtg
    65281 ccgtcggcgg gttggctggc gaacagaccc acagcccgtg cggctccgac catccccgcg
    65341 ccgcagagcg cttcaaactt tttccggaga ctcagctcat gtcatcgacc gaaaccacga
    65401 ccaccaccgt caagacctac ctgcgcccga tcgaccgcga tggcctgctc gccttcgccg
    65461 agaagggccg caacaacccg gcctcgcgtg gcaccaacaa ggtccatacc gtggtcgagg
    65521 gccagtaccg cacattgagc tacgtgggca accacgcgcc ggtggtggtc gatgaaccgc
    65581 cgcacctgtt cggtcaggac accgcgccgg cgcccggcga gatcgtgctg tcggcgctgg
    65641 gcggctgcct cgcagtgggc atcaccgcag tggccacctg gaagcaggtc aggctgagca
    65701 aactggagct gttcctcgag ggggacatcg gaaaccccgc ggcctggggt gccggcggcg
    65761 cgctgaaggc cccggccgac atgggctttc aggcgatccg cgtgaaggtg gtcgtcgaag
    65821 gcgacgccac tcgcgaggtg ctcgacgaaa tcgtcaagca cgccaacatg tattcgcctg
    65881 tggccaacag catgcgcaac ccgatcccgt tccagatcga tctgg
//
LOCUS       DAOORV010000006        62027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_513_length_62027_cov_9.203301, whole genome shotgun sequence.
ACCESSION   DAOORV010000006 DAOORV010000000
VERSION     DAOORV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 62027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_62027_cov_9.203301"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKV56_01670"
     CDS             complement(<1..424)
                     /locus_tag="PKV56_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66543.1"
                     /translation="MTTRRVFLFEYLSAGTPDRCHGSAAGLHAEGLAMRDAMQADLAA
                     LPNVELSVAVGRSDSSFGADGPASTRQVCALVGESALDFVARQADLHDLVWVVAPETD
                     GLLAALNEVVPPQRWLGCTGEAIRLASSKRATLARLQQH"
     gene            461..1186
                     /locus_tag="PKV56_01675"
     CDS             461..1186
                     /locus_tag="PKV56_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOX66544.1"
                     /translation="MNLIPVIDLMRGQVVRAHRGARGSYRPIESRLCASSDPITVARV
                     LRDHCDADQLYIADLDALTGGRPQVAILRSLQRALPQTELWVDAGCGDARVANELLEA
                     VDAPAGCLIPVLASESMRSRQAFEQAFGPAGDDGSHARKDWVLSLDRRDGVRLDAAGC
                     WTLPALWPTRVIAMTLEQVGTDAGPDLETFAALSQLNASVRWFGAGGVRDPGDLERAR
                     AAGASGWLVASALHDLRLPPMRR"
     gene            1347..2672
                     /locus_tag="PKV56_01680"
     CDS             1347..2672
                     /locus_tag="PKV56_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HOX66545.1"
                     /translation="MSASVSTATSGSGSDTAWTCPFCPLLCDSFQVAIPADQAALALR
                     GSDCPRAGRALQRFSGAPSVASAQIDGKSCDLDSAVAAAAQVLATSRQPLFGGLGTDV
                     AGARALYRLACETGAICDAAQGDALMHGLRALQDRGQFTSTFAELRTRADLIVCLGGS
                     PAVQHPEFFRRCGVGEDLVGARHIVLVGAAAGDDVPATLAKLNGARGVTAEAIDLHGD
                     LFDTAAMLAALVANHAVSAAPAALVALARRLHAAQYAVVVWQNPELPAHGALLVETVN
                     HIVGLLNRSTRAAGFPLGGGNGAATVNQVFSWLSGLPLRSRVGPTGIEHEPLCFDARR
                     LLADGAADALLWLSSFNADALPSADLPRVVIGHPDLMQHLSASGAGHRTVFIPVSTPG
                     IGSSGHLFRADGVVLMPLDAVYADTLPTAASVIWRLTTAVAALKQGAAA"
     gene            2669..4354
                     /locus_tag="PKV56_01685"
     CDS             2669..4354
                     /locus_tag="PKV56_01685"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOX66546.1"
                     /translation="MTTVRLKGGRVIDPTHGVDDVVRDVCIRDGRIVELAPNEAVTTE
                     IDASGCVVMAGGIDMHSHIGGGKVNLARMLLPEDHRARGNPYALPENRLELASCGTCA
                     PGTLATGYRYVEMGYTAAFEPAMVSANARHAHMEMGDVPILDHGAYVMLGNDELFLQM
                     LAEGRDFQQIRDYMGWTIHASKAMGVKVVNPAGISAFKFNQRKLDVDEKHVHWQVTPR
                     QVVNTLARALGELGVPHPLHIHGSNLGVAGNIESTLETISALDGLPGHLTHIQFHAYG
                     TEGPKKFSSAAVRLAEAVNANQNISIDIGQIMFGQTVTASGDTMRQHANSGIGTPRKW
                     IGADIECDAGCGVVPFRYREQSYVNALQWSLGLEIFLLVDDPWRVVLTTDHPNGGPFT
                     SYPHLIRLLMDRSFRDEQVAKLHPDIAANSALRSITRELSLYEIAVLTRAGPARLLGL
                     RDRGHLGAGAAADIAVYRDAADREAMFTTPEYVFKDGTLVSRAGRIVAVPTGGTHFVE
                     PDYDRGIEKTLRKHLAAHGSVNFDHLTISHDELCSCCNGGRLLPTECFEGATT"
     gene            4351..5322
                     /gene="fhcD"
                     /locus_tag="PKV56_01690"
     CDS             4351..5322
                     /gene="fhcD"
                     /locus_tag="PKV56_01690"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HOX66547.1"
                     /translation="MSAASADIAKAPLVINGVVIDDTFAEAFPMKATRIVITAHNLTW
                     ARHAAVATTGFATSVIACGCEAAIERELDASETPDGRPGISVMLFSMSGKELAKQLER
                     RVGQCVLTCPTTAVFAGIADGADVIDRIALGKNLRFFGDGWQISKMIGGQRYWRIPVM
                     DGEFVAQETVCVTKAVGGGNLLLLARDTDSALAVAEAAVLAMKRVPDVIMPFPGGVVR
                     SGSKVGSKYPSLGASTNDAFCPSLIGLAAKTELTPETRCVMEIVIDGLTDTAVGAAMR
                     AGIDAGTHIGAVGGLLRISAGNYGGKLGPYHFHLHKLLGQGAQGGQS"
     gene            5319..6149
                     /locus_tag="PKV56_01695"
     CDS             5319..6149
                     /locus_tag="PKV56_01695"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HOX66548.1"
                     /translation="MSTTITLTLKQAPALRVDLRGVTPAALAALSVGAVERLSVTHGT
                     QILPLAELFGVVRSDSAGDVDELRLIGDLAHFDRVGWQMAGGRVFVDGSVGDYVGGGM
                     SAGELQVAGSAGSLAACEMAGGLLTVTGDVGDFAASTLPGSMDGMRGGTFIVKGNAGA
                     RFGDRMRRGNALVFGNAGDFLASRMVAGTIALGGEAGPHAGYGMRRGSVVFAASNAAG
                     DIAQTFVPAVADAPVFWQLLSRDLARHGGPFAGLPARSIRRYLGDVAAGGKGELIVAG
                     "
     gene            6254..6787
                     /gene="fae"
                     /locus_tag="PKV56_01700"
     CDS             6254..6787
                     /gene="fae"
                     /locus_tag="PKV56_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOX66549.1"
                     /translation="MSKNYIFHAGEATVLAKDGQFTDAMPEILIGSTSGPVGHAFANM
                     MAQSKGHTAMFAIRACNQLVRPATIVVPKVTLKDSANIDLFGGVVQSATADAVVDCLI
                     DGTIPKDIANELCIISLVWIDPRCAKDPNLDRRDMYRTNHEATMLAIKRALNNEPSVD
                     ELIANRHKIKHDMDDWS"
     gene            6938..8842
                     /gene="pabB"
                     /locus_tag="PKV56_01705"
     CDS             6938..8842
                     /gene="pabB"
                     /locus_tag="PKV56_01705"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOX66550.1"
                     /translation="MDRCCALLDDRDATPERPTSRLYTAFVREHRCIDPATLDATWAQ
                     VEADLRSGLHALVLGDYEWGVKLLKAGHRTLVPGDRSALRVLMFADLALLSRAEADEW
                     LERLEAAGDVETVVPGAAGVMHLEASVDQPAFTRAIDRIHEAIQAGETYQVNYTYRLH
                     GQAYGSPVGLYRRLRARQPVAFGAYIELPTSDAHDDATTHVLSCSPELFLRNEAGLLT
                     ARPMKGTASRIRAPEADSETARHLSLDVKNRAENLMIVDLLRNDIGRVAQIGSVTVPD
                     LFAIEPYSTVFQMTSTVQARVRPDVGFPELLRAVFPCGSITGAPKHHTMELIAELETT
                     PRGLYCGAIGWVDAPRADARLGDFCLSVTIRTITLGADVAGLRPARLGIGAGIVLDSR
                     AEDEFDECRLKARFLTGLPPGFDLFETMLATAETGVRHLDRHLARLGRSARALGFVFD
                     PVEAQRLLDARLGELCAREAARHRLRLSLAHDGRLQLTHAMLTDLPPAADGVTLLVAD
                     HRLPNSHPLSGHKTTLRQHYDEGVRAAERAGAFDSLFFTEDGRLVEGGRTSVFLKLAG
                     RWCTPPVADGALPGVMRGLVLEDPAWQAVERSLARDDLDRAEAVMVCNALRGVLPARV
                     MATPDRSAAA"
     gene            8839..9516
                     /locus_tag="PKV56_01710"
     CDS             8839..9516
                     /locus_tag="PKV56_01710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX66551.1"
                     /translation="MNLALFDLDHTLIPFDSGMAWTQFLIAKGVLPSTAADTYLGYCH
                     QYVAGTLDIHDMHRANVSPLARYPMAQIRQWGDEFEAHLASRVPAPMLALVQSHRDAG
                     HLCALVTATTRFIAEPLGRLFGLPHVVATEPVIVGVAHDGRLTGEISGLPCFQQHKVT
                     RVEQWLTAGSGAAGWHPDLRSFERSWFYSDSATDLPLLQAVTDPVAVTPDARLRAVAI
                     EAGWPIL"
     gene            complement(9574..9918)
                     /locus_tag="PKV56_01715"
     CDS             complement(9574..9918)
                     /locus_tag="PKV56_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66552.1"
                     /translation="MTHQRLVALSLLTLTLATGAGAQTLTLGAGPYPTMVRDVGPVVT
                     QSYLIRHPASPSVRAGHANFEHPAFSARAERGVDANTFIVGHPAVPSSPLGTGATAPE
                     VVPVASLPIVVR"
     gene            complement(10128..10550)
                     /locus_tag="PKV56_01720"
     CDS             complement(10128..10550)
                     /locus_tag="PKV56_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX66553.1"
                     /translation="MKKTLGAMLSFLTALGAGSAAAQTTPETAASAPATALYTVVDGY
                     KVDAQTMKGFRTWRSAACDRCHGANQEGMVGPSLIESFKTLTKEQVVTTVTNGRLEKG
                     MPSFSASPVVMENIDHLYAYLKGRSDGAITRAKVEELK"
     gene            10786..11067
                     /locus_tag="PKV56_01725"
     CDS             10786..11067
                     /locus_tag="PKV56_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66554.1"
                     /translation="MKLENIVFAVAAKIDIDRSFVFEYVVCRHIQSLLLNRTALPSLA
                     IDVEGLEYAESPVLHQCIDRAAHLFLADLVRASEMCEAAIARWQLTFET"
     gene            complement(11091..11651)
                     /locus_tag="PKV56_01730"
     CDS             complement(11091..11651)
                     /locus_tag="PKV56_01730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX66555.1"
                     /translation="MMNGSTLFLDTLTGLPNRACFLDRLSEAADEARTHKRALALLLI
                     DLDNLFLINLEAGRHGGDQALVALAQTLRRSARAHDFVTRLGGDQFAMLLPGTSLATA
                     AQLASAIRGLVGRVHEIHPGFGVIDVCIGVSARPSGDDWRAQDLLDLADLRLTSAKRS
                     RHRPGTPQHVWAGPPDPDDDDDANEG"
     gene            complement(11681..12247)
                     /locus_tag="PKV56_01735"
     CDS             complement(11681..12247)
                     /locus_tag="PKV56_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66556.1"
                     /translation="MTLLAMLWWLPPVSAAEVAPGSGELTLYGVRLKDASVAAFVSAA
                     TAAGARQLERRPEGPVAFDTRDAGVPALQRLTLIADQGGFVSAQFIIKSYGQENEALR
                     QLLVQKYGLPTTADGARRPFPSFAGRFAPRGTYDWDFAGGMKLIYRQPAIGDATLSYT
                     DVEKARRLGSPAASGAEPRAKGDLADRF"
     gene            12442..13620
                     /locus_tag="PKV56_01740"
     CDS             12442..13620
                     /locus_tag="PKV56_01740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX66557.1"
                     /translation="MRLNLPLGRKRVAGTGFDLGAEKSRVAEALVRSASRILESRDDR
                     DIIHSVCEALTLATPHVRLAWTWFGPLDTPRIVPQVVVGPAADYARKLSIDRSFFTEI
                     GSAFRSLVGRKAEAFNVSRMSLYGPWREAAATHGIRSSLALPLASTVNEDRGLLALYA
                     DQPDYFEQVGIGLFESLAHMCSAVLSRSAERARPREPAHSDALTGLFSRHAIDAVARP
                     ILRNDPLDPRASALLIDIDQFSRINDEYGPACGDTLLCSLADRLRRGLRKGDDVFRWE
                     GAQFLVCLPRTDLASATLVAYKLLDAIAQPAHTLADGRSVPLTVSIGVAELGVDEAIA
                     GAVVRADAALREAKRQGCSRVCKAPALVVGGGPPQVDARNAPGRRVITGITRTRASLP
                     "
     gene            complement(13592..13882)
                     /locus_tag="PKV56_01745"
     CDS             complement(13592..13882)
                     /locus_tag="PKV56_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66558.1"
                     /translation="MIVRIEIDSPQGHSFDYRVTYEGEALFADAELASLTDALVAAVE
                     GLAPEVVGAEVWYLGIVSGTYPLHVIARNHEQIAAHASNTTEAIREAMPEFE"
     gene            complement(13969..14772)
                     /locus_tag="PKV56_01750"
     CDS             complement(13969..14772)
                     /locus_tag="PKV56_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66559.1"
                     /translation="MTSIDTFPEVPERYRGVWRRTLLETPDIRDTTTWVHWLQTPRWH
                     ADLRVPAAAEVDRAAIALDAMSGEQHLALAHQQGFFGRTEVVQHLHGDVCTWHRRLDM
                     QPPGLTPDAGWMEFGSDDRVIETGVHGAYREVWERLPGSIGPALVLAETSLSPDDEAG
                     VLSTARAFLLLSGECLMRVRARRIGWPAGTDPGNTLSTLLSRDPASAPALLDFEISYG
                     LLRDGRWFIERSTLPELEGHTEPMHWQPAGATRFEVTCAAGTSVWCRLD"
     gene            complement(14813..15619)
                     /locus_tag="PKV56_01755"
     CDS             complement(14813..15619)
                     /locus_tag="PKV56_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HOX66560.1"
                     /translation="MTQVRKLWPLLTATHRYDKSISTWQRGRGQQIEAPILAYLIETA
                     NGRILYDVGCDHEKIADPGSRAYHYNPATFEFGAPDMRDEQRIPRHLARLGLTPADID
                     VIFIGHLHFDHAGGLRDLRRCGCGAEIHVQRDEMEVGISGADPAVFADDLIDEAGAPL
                     QFRLQTGEYTLVPGVQAVATPGHTIGHMSMWVEMPKGPPILLAGDAADLQENIDDEIA
                     PGTLWQGREAEAIDSIRKLKALAAETGAWLWPNHDFAFYRSLPAFPQFID"
     gene            complement(15616..16272)
                     /locus_tag="PKV56_01760"
     CDS             complement(15616..16272)
                     /locus_tag="PKV56_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HOX66561.1"
                     /translation="MLDAARTALLVIDLQRDFLDPLGYAARAGVDVSRLRATLGPVCA
                     LIDAAREAGLHIVYTREGHRPDLSDCPAAKLARSRNAGAEIGSAGPLGRLLVRGEPGH
                     GLVLELQPRADEPVVDKPGYSAFHQTDLDQILRGWGTETVIVCGVTTEVCVHTTLRAA
                     TDHGLHCITVGDACAASEAALQAPSLAMIAVEGGIFGTVTDSATLVARLLNCKDQELP
                     "
     gene            complement(16285..17049)
                     /locus_tag="PKV56_01765"
     CDS             complement(16285..17049)
                     /locus_tag="PKV56_01765"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047789 - creatininase activity [Evidence
                     IEA];
                     GO_process: GO:0046449 - creatinine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HOX66562.1"
                     /translation="MSTVWMNQLSWVDYEARLKLHQPAILLPVGALEQHGPHLPLGTD
                     GLLSAAVAADAAARIGGLVAPTLSYGYKSQPKCGGGQHFCGTTSVDAATLIGSVRDAV
                     REFARHGATKLVIVNGHYENQWFLTEGIDLGLRDLGPRAALQVMRLEYWDFLSEDTLA
                     SVFPEGFPGFALEHAAVIETSMMLHYHPNLVRLDLIPDEPPADFPPYDVYPTRRHWVP
                     LSGVLSSARGSSVEKGAVMAQELSTRIAAAITHEFG"
     gene            complement(17046..17975)
                     /locus_tag="PKV56_01770"
     CDS             complement(17046..17975)
                     /locus_tag="PKV56_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX66563.1"
                     /translation="MTVTSSPLISRLAPPSPGTRLPTLVLLASAVLWGLLWWPLKGFA
                     GVGLSGPMLSLLSYGAVGLLGIGLLWRERPAWKPQTALLLLLMLVGGWANTAFVQALV
                     MGDVVRVMLLFYLAPVWSVLGGRLFLGEAISRRRALAVVLALVGLWFVIGGSAAWAKP
                     LGTADWLALSAGLAFAGNNLISRAAQAIPMTSKTVAVFFGCGLLSGGSVLMAGAEMPP
                     ITALIALALAGYAFIWLLLATVTWQYGVTHLESGRAGLILTVELLVAVVSAVWLGEES
                     LRPIEWAGGALIAVAALIEATDSSESTSKEKAG"
     gene            complement(17992..18777)
                     /locus_tag="PKV56_01775"
     CDS             complement(17992..18777)
                     /locus_tag="PKV56_01775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOX66564.1"
                     /translation="MNAAASLPPQAFQLALTGRVVLVTGAASGIGRATALACGRAGAR
                     VAINHLGRAEEAGALVAELEAGGARAFAVEADVSLARDVAAMVKAVASELGPIDALVN
                     NAGVIQDRPFLETTEADWDRMLDTDLKSVFLMCKAVLPGMLARQAGVIVNIASDLGIL
                     GRAQFAPYCAAKAGVIGLTRSLAREFAPDIRVNAIAPGPVATAMLSPEHMSPEWIEKE
                     LDIPQHRFAAADEIACTALFLLSDLSRFYCGQVLSPNGGSVMA"
     gene            complement(18774..19595)
                     /locus_tag="PKV56_01780"
     CDS             complement(18774..19595)
                     /locus_tag="PKV56_01780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX66565.1"
                     /translation="MSIDPSARAGGRTAPCLGLQGLTVVLSGAAGGIGQALAAAFAAQ
                     QVRLIITDRDAGALQRVADSLEAHHVTAIAADLGPDSALQALASRIQASHGTVDVLVN
                     NAGTEYPTPLDDTHPQATERWSALLENNVGSMVRLTRTLLPLMPRGACVINQSSIWGL
                     TGVADFSAYVASKHAVIGLTRSLAWELAPLGIRVNAVCPGWIRTAAALRSLTSMATAQ
                     GRSESDVEREILARQAIPRMLEPEDIAGVFLFLASDAARSLTGQAISASHGEVMN"
     gene            complement(19598..20605)
                     /locus_tag="PKV56_01785"
     CDS             complement(19598..20605)
                     /locus_tag="PKV56_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOX66566.1"
                     /translation="MSFRTPDGAAPNIAALALTLLGFPAGIAMLTAASWVLNALGFAL
                     VTLTLVWSAYYIHEFAHHAIFRSPQTNERWGTLMTWLNGSCYAPFADLRRKHMRHHVE
                     RADVITFDARGFLLAAPAWLRRGVLALEWAYFPAVEFLMRGFVVAMPFRAGGSNAARA
                     RIIGIAVVRLGAFAALGMYSPKALALYFVAYLLFVTVLRFADCFQHTYDAYPILGDAP
                     IPDDKVRDRAYEQANTFSNVVGLDNFWLNLLWLNFGYHNAHHERPTVPWHRLPAYHRE
                     IYGNAYGQVIPVPQLLKAFHQHRVTRVLSSDYGEVRGPEVPGRADGFIGAVGVSFLTA
                     V"
     gene            complement(20602..21651)
                     /locus_tag="PKV56_01790"
     CDS             complement(20602..21651)
                     /locus_tag="PKV56_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX66567.1"
                     /translation="MELIRPLRSSALSSALWRRLGSSLAKPAAGLALAAFAVASHAAD
                     VKPMKIGTVVWIGYGPFYVAEKLDLFKKHNLKVSLQIFTDPALIPSAIASGAVDGGML
                     TYDQVVGQVAAGRPQKVVMPIDYSNGGDAIVADKSIAKVADFKGKKVGFNPLSPSDFL
                     LSYALKVNGLSDKDITAVNMTPEAVPAAMASGQLSIGVTYEPSLSQIVSQGGGNKFKV
                     VFSSKDAPGLIADVLVFDDKVIKAKPTEIAGVIKGYLDGLAYIKAKPDDAAKIIGKAM
                     GVSAKEVKEQWSGVYNIPLAEMPKAFTKAPETTSYYASGEIISQLLKAKGQITTVPAT
                     EATFDAQFVTEMVKK"
     regulatory      complement(21707..21821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            complement(21934..23766)
                     /gene="atzF"
                     /locus_tag="PKV56_01795"
     CDS             complement(21934..23766)
                     /gene="atzF"
                     /locus_tag="PKV56_01795"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HOX66568.1"
                     /translation="MRPDLSLPALRTRYLSGELTPTRLVTELHARIDAEEAALPRNVW
                     IHRLGLDALMRYASALEGRDPATLPLYGIPFAIKDNIDLAGVPTTAACPAFAYTPQRS
                     ATVVQRLIDAGAIPVGKTNLDQFATGLVGVRSPYGACRNSIDPAFVSGGSSSGSAVSV
                     ALGLASFSLGTDTAGSGRVPAAFNNLIGLKPSLGRIPTTGVVPACRTLDAVSVFALTA
                     ADAATVLASAQGPDAQDAYSRTMPPHGRNFGATSFRFGVPRPQDLVFYGNDSGTAQFA
                     QAIQHLRALGGMPIEIDLTPFRDVARLLYEGPWVAERYAAIRTFIEAQPEALHPVTRA
                     ITEAGARLTAADTFSALYRLKELQQTSLAAWGDLDCLVVPTAPTIHTIAAVEADPIAL
                     NSQLGHYTNFVNLLDLSAVAVPVGFADQGPARGLPHGVTLIGRCGADLPLLTLASRLH
                     ARTVATVGATPHAPHLSAGAPDARGFPSGQVQVAVCGAHLSGLPLNWQLTQRGAMLVR
                     AVRSAPVYRLYALPGGPPFRPGMVRVDANDTGGGAIELEVWELPASEFGSFVAGIPAP
                     LGIGTVLLEDGTSVQGFVCESHAVRDAKDITALGGWRAHLSASS"
     gene            complement(23790..27431)
                     /gene="uca"
                     /locus_tag="PKV56_01800"
     CDS             complement(23790..27431)
                     /gene="uca"
                     /locus_tag="PKV56_01800"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HOX66569.1"
                     /translation="MFDKVLIANRGAIACRIIRTLRRLGVGSVAVYSEADAGALHVSM
                     ADESVCIGPAPVASSYLDGARIIEAALATGAKAIHPGYGFLSENAGFVEACEAAGLVF
                     LGPAPQQMRLFGLKHTARALATDSGVPLLPGTGLLSDLSEALARAEQIGYPVMLKSSA
                     GGGGIGMQMCAGADELRSAFDTVRRLAQSNFSDAGVFIERYITDARHIEVQVFGDGAG
                     QVLALGDRDCSLQRRNQKVVEEAPAPHLRPEVRAALHATARRLAQAAHYRSAGTVEFV
                     VDASTQDFYFLEVNARLQVEHGVTEAVTGIDIVEWMVRLGAGEMPALDSLRVTQTGHA
                     VQARLYAEDPFKNFQPATGVLTRVEFPADLRVDTWIERGTEVTAYYDPMLAKLIAHGS
                     DRADAIAKLSKGLAVTHVDGLESNLEYLRAVMSFAPFADGMHTTRSLASFSAPSRAFD
                     VLSPGVQTSVQDWPGRLGYWDVGVPPSGPMDPLALRLANRLLGNEQGAAGLEITVAGP
                     SLRFRCPAVVAVCGAPIELSLDGKAIPMGRAVTVEAGQNLRIGRVGQSGAAGCRAYLA
                     VAGGIDVPEYLGSGSTFTLGQFGGHGGRVLRGGDVLHLGQPVSATPAPISAEQAAMLT
                     PACGTHWDIGVLIGPHAAPDFFTPDDIATFFATDWQVHYNSSRTGVRLIGPKPTWARS
                     DGGEAGLHPSNIHDNAYAIGAIDFTGDMPVILGPDGPSLGGFVCPATVVQAELWKLGQ
                     LSPGNTVRFKPLTLAEARALAAAQDVVLSATAPLANTAPAAAPISRALLSDAAVLHHR
                     AARDDGIEIVYRRAGDAYVLIEFGELKLDLTLRFHVHALMQLLESWRDEGRLAGVIDL
                     TPGIRSLQVHYDPSRIALATLMDALLDAQDGVPATADIEVPSRTVWLPLSWDDAATRL
                     AIEKYMQSVRADAPWCPSNIEFIRRINGLDDIQAVYDTVFEASYMVLGLGDVYLGAPV
                     ATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCVYGMEGPGGYQFVGRTVQMWNRW
                     KSTSEFEPGKPWLLRFFDQLRFYPVSADELLKLRSDFVAGRAGLRIEDGTFRLADYQR
                     YLVENGAAISEFKTRQQAAFEAERERWRAAGVAETVEETVDADLDARAAAASAFDGEV
                     ITSEVSGGVWSVLAAKGSAVQAGDTLLVVESMKMEITVLAPRAGVVHEVLCSEGQPVT
                     AGQPLVLLR"
     gene            complement(27506..28180)
                     /locus_tag="PKV56_01805"
     CDS             complement(27506..28180)
                     /locus_tag="PKV56_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HOX66570.1"
                     /translation="MAATVESPLLPADAVIDEICLAGDPWAKVVKKGQTLRIVDIEGN
                     QAVDTIFYSAADVGERYSLTHTIQAQGALYLTVGSVLMSSEARPMLTVTADTCGRHDT
                     LGGACACESNTVRYALEKRYMHSCRDSFMLALARIDSGSESTAGIPRLTKRDVVSNIN
                     FFMNVPVTPEGKLTFEDGISAPGKYVEMRAEMDVVVLVSNCPQLNNPCNGYNPTPVRF
                     LVWDPA"
     gene            complement(28180..28962)
                     /locus_tag="PKV56_01810"
     CDS             complement(28180..28962)
                     /locus_tag="PKV56_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HOX66571.1"
                     /translation="MQTHFNSLSPLARAAVPDRSTPYAQDLVLWDETLPGGAHWSGLL
                     RRGNTLRLIDVEGRANVSALFFNQEEKTERYNMPDTLKAQHTAHLTRGHALYSDMGRV
                     LCSIPDDSCGWHDTVCGVIDADTVRQRYGEASYQQRRNDMHRNGQDGLLVELGKWGMD
                     RRDLVANINFFSKAVADETGRLHFDTAHRAPGQHVDVRFEMNVLVILSAAPHPLDPAT
                     SYAPTPVHLTAWRSGTAGADDACRLHCPENGRGYINTERWFL"
     gene            complement(28990..29856)
                     /locus_tag="PKV56_01815"
     CDS             complement(28990..29856)
                     /locus_tag="PKV56_01815"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66572.1"
                     /translation="MQSIAPQVPSPKAEIRRLGKVFSGAAAGTPAALQDIDLSIEDNE
                     FITLVGASGCGKSTLLRILAGLEHHTSGEVICNGRPVSGPGAERAMVFQHYSLYPWLT
                     VMENIKFSRTLQVNRENLTSSDVEAASGRAEALLNLIGLAKVAKSYPSQLSGGMQQRV
                     AIARALMGRPQVLLMDEPFGALDAQTREVMHDLILHIHRIEKTTIVFVTHDVDEAIYL
                     GQRVVLMEPRPGRIHSIYPVPLPARRQQDMKHGAPFRELKAEILKHIRESSGMATDLD
                     LLERISQQGQSL"
     gene            complement(29871..30731)
                     /locus_tag="PKV56_01820"
     CDS             complement(29871..30731)
                     /locus_tag="PKV56_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX66573.1"
                     /translation="MSATAPPMGSNFTPHAGAAKPPPTPSIWAIAGRLSRTQYWAFAA
                     LGLILPFAVWALLSASGWIDPVFMPGPGSVFARLGTWLMEDDLIADTGISVYRVLAGF
                     ALSAAFALPIGLLIGAFRPVEALLEPLTDFIRYMPAVAFIPLVMLWFGIEESAKIAII
                     FIGTFFQMVLMVAENVRLVPTAQIEAAQTMGATRDEIVRLVLLQSAKPAILDTLRITM
                     GWAWTYLVVAELVASNSGLGYAILKAQRFLKTDTIFGGIILIGVIGLLMDQAFRWLHR
                     RAFPYMHFKR"
     regulatory      complement(30791..30899)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            31431..31595
                     /locus_tag="PKV56_01825"
     CDS             31431..31595
                     /locus_tag="PKV56_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66574.1"
                     /translation="MMSSSKMYVCRRCHANFKTGDGKPDLRMPGLGKCNACVAASAAA
                     AAAASGSAPT"
     gene            31592..32335
                     /locus_tag="PKV56_01830"
     CDS             31592..32335
                     /locus_tag="PKV56_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX66575.1"
                     /translation="MIESPASPERAGDPGDLALVFGARGGLGAALRDRLRGSARYAQV
                     LAGGRGGTVDMAIDITDESSIESAATQVAERLQQGSILRLVIDATGFLHGRGFEPEKS
                     LRQIDAAHMAHAFAINAIGPALLMKHFLPLLPRSGRSVFATLSAKVGSIGDNHLGGWY
                     SYRASKAALNQLVRTAAIELSRRQPQAICVALHPGTVATPLSSPFAKSGLQVQAPDLA
                     AQRLLAVIDALTAEDSGGFFNHQGERLPW"
     gene            complement(32355..33128)
                     /locus_tag="PKV56_01835"
     CDS             complement(32355..33128)
                     /locus_tag="PKV56_01835"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOX66576.1"
                     /translation="MSAFNEERVLSVHHWTDRLFSFTTTRDPSLRFSNGHFTMIGLRV
                     DGKPLLRAYSIVSPNHDEHLEFLSIKVPDGPLTSRLQHIQVGDSIIVGKKPTGTLLID
                     YLLPGKRLYLIGTGTGVAPFLSIIRDPDTYERFEKVILIHGVREVKELAYHQYLTEEL
                     PAHEFLGEMVTNQLLYYPTVTREPFVHQGRVTTLLDNGKLAADLGLPALDPEHDRVMI
                     CGSPEMLRDLKAMMEARHFKEGNTSKPGDYVIERAFVEQ"
     gene            33271..34461
                     /locus_tag="PKV56_01840"
     CDS             33271..34461
                     /locus_tag="PKV56_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOX66577.1"
                     /translation="MTDHAAARFQWEDPLLLDSQLAADERQVREAAAAFCRDRLAPRV
                     LEAFRHETTDVAIFREMGELGLLGIVIPPEYGGAGMNHVCYGLVAREVERVDSGYRSM
                     MSVQSSLVMLPIHVFGSEAQKQQYLPKLAAGQAIGSFGLTEPDHGSDPGGMLTRARPV
                     AGGWRLSGTKTWITNSPIADVFVVWAKDDAGAIRGFILEKGWPGLSAPALHGKVGLRT
                     SVTGQIVMDEVFCPAENALPDVRGLKGPFTCLNSARYGIAWGALGAAEDCWLRARQYV
                     LDRQQFGRPLAANQLIQKKLADMQTEIALGLQACLRLGRMKDDGSAAPEITSMLKRNS
                     CGKALDIARMARDMLGGNGISDEYGIARHLVNLEVVNTYEGTHDIHALILGRAMTGIA
                     AFAN"
     gene            34595..36283
                     /locus_tag="PKV56_01845"
     CDS             34595..36283
                     /locus_tag="PKV56_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HOX66578.1"
                     /translation="MPLSQPLSAEATEPRRRGPARLTWSLPTEAVVLMASLFFALVSN
                     QPFWSAAMAGRRLDDPGSWLFGACAFLLLLVIHFVVLSLVSTRRTVRPLLTVLLLGTA
                     AASYFMGRYAVFLDPTMLRNLLHTDTAEARDLLAWDMLPHLTLQAALPIWLLWRIRIC
                     DRPWRSAALWRAGAVAGGLLIGVAALLTVSQDLSSLMREQKALRYLATPANYVYSLTR
                     VATADAVGATRPLTQIGLDAVRGPAWSRYKKPLLFVVVVGETARAANWGLNGYARQTT
                     PELAQLGVFNFTDVTSCGTNTETSLPCMFSHIGRRDYDEARIRGEESLPHVLRHAGFE
                     VEWRDNQSGCKGICAGLPSQNMAKSTDAKLCDGHECLDEILLQGLDAQMANDQGSRVV
                     VLHQMGSHGPAYHRRSPAAFQQFKPTCDTSELRKCTKQQVVNSYDNSVLYTDHVLAKT
                     IGFLKSQSDRYDTAMLYVSDHGESLGENNLFLHGMPYAIAPKEQTHVPMVMWFSDSYA
                     SSRGIDLGCLRGRAVQHASHDDLFHTVLGMLDVQTKTYEPALDVLRTCRVAAAP"
     gene            complement(36229..37221)
                     /locus_tag="PKV56_01850"
     CDS             complement(36229..37221)
                     /locus_tag="PKV56_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX66579.1"
                     /translation="MPLSKNATFRQLATFHTVARLNSVSLAADELHLTQPAVSIQIGI
                     LEESAGTPLLMRTGRGIRPTAAGELLAKYAGRILELWREAGEEMATLRGVFSGTLRVG
                     AVTTAEYLLPPLLVAFANDNPKIKFNLQVGNRDEIVRMLAGHEIDLAIMGRPPAELKT
                     TSLVFAKHPMGFLAAPNHPLMSHQGLSLSDLGKERILVRERGSGTRTTVERIFKDADL
                     QLRIGSEMSSNEAIKQMCAAGFGVAFLSMHTCVLERQGGVLQLLPMPGNPIERDWCVM
                     HLSSQQLPQVALAFKEFLRDHGQERISAQLAMPKVKPQGAAATRQVRKTSSAGS"
     gene            37363..38829
                     /locus_tag="PKV56_01855"
     CDS             37363..38829
                     /locus_tag="PKV56_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HOX66580.1"
                     /translation="MGHMNEPQQITDSKKRYSAGVLKYAQMGYWDGDYVPKETDILAL
                     FRITPQDGVDPIEAAAAVAGESSTATWTVVWTDRLTACDMYRAKAYRVDPVPNTPGQY
                     FCYVAYDLSLFEERSIANVTASIIGNVFSFKPLKAARLEDMKFPVAYVKTFPGPPTGI
                     VVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPFMH
                     WRDRFLFVMDAVNKASAATGEVKGSYLNVTAGTMEEMYRRAEFAKELGSVIVMIDLVI
                     GYTAIQSMSDWCRNNDMILHLHRAGHGTYTRQKNHGVSFRVIAKWMRMAGVDHIHAGT
                     AVGKLEGDPMTVQGYYNICRDTYTKVDLPRGIYFDQDWGALRKVMPVASGGIHAGQMH
                     QLIDLFGDDVVLQFGGGTIGHPQGIQAGATANRVALEAMVLARNEGRDIKNEGLDILR
                     DAAKWCSPLKAALDTWGEISFNYQSTDTSDYVATPSVS"
     gene            38853..39284
                     /locus_tag="PKV56_01860"
     CDS             38853..39284
                     /locus_tag="PKV56_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HOX66581.1"
                     /translation="MRLTQGTFSFLPDLTDAQISKQIDYCLSKGWAIGLEYTDDPHPR
                     NTYWEMFGNPMFDISDPAAILMEVNACRRTFPDHYIRLTAFDSSHGTESVVMSFIVNR
                     PKHEPGFRLVRSEQAGRTVRYSIESYATQSRPEGMRYSSDN"
     gene            39303..40217
                     /gene="cbbX"
                     /locus_tag="PKV56_01865"
     CDS             39303..40217
                     /gene="cbbX"
                     /locus_tag="PKV56_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HOX66582.1"
                     /translation="MDASTPPSSTPETPRALLESSGVKTVLDQLDAELVGLVPVKSRI
                     RDIAALLVIDKLRQDMGLQAQPPSLHMCFTGNPGTGKTTVAMRMAEVLKQLGYVRKGH
                     LVAVTRDDLVGQFIGHTAPKTKEVIKKAMGGVLFIDEAYYLYRPDNERDYGQESIEIL
                     LQVMENHRDDLVVILAGYKDRMETFFGSNPGMASRIAHHIDFPDYSEAELMQIARLML
                     EKLNYRFDADAEPVFGRYIGLRRQQPHFANARSIRNALDRIRLRHATRLFAHDTSLTR
                     DQLCTLQAEDILASRVFAAAAPPVTAGN"
     gene            40219..41028
                     /locus_tag="PKV56_01870"
     CDS             40219..41028
                     /locus_tag="PKV56_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX66583.1"
                     /translation="MSIEALIFDVDGTLADTEEAHRTAFNLAFERFRLGWVWGRDEYR
                     ELLKITGGKERITHYLRSLTTVGPAEFKRLLDLVPDLHAEKTRFYSKVVGDGGVPLRE
                     GVARLLDEALNDGCRLAIASTTTAMNVDALLQANLGSRGLEKFSVIACGDQVRSKKPA
                     PDIYLLALKHLGLHAEQAVAFEDSYNGLRSAVSAGLRTIVTPTFWTEGSDFGAATLVL
                     PTLGSPAHPLIGEPGGRLRNEGWLSFAEVVQLTARPKLPSAVRALYQGTTS"
     gene            41025..41753
                     /gene="rpe"
                     /locus_tag="PKV56_01875"
     CDS             41025..41753
                     /gene="rpe"
                     /locus_tag="PKV56_01875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOX66584.1"
                     /translation="MNSASPHPEMRIAPSLLSADFARLGDEVTAVIEAGADVIHFDVM
                     DNHYVPNLTVGPLVCQAIKPYAVRSGQPVPIDVHLMVKPVDALIPMFAQAGASIISFH
                     PEASEHIDRTIQLIKSLGVKAGLVLNPATPLSCLDHVLEQLDLVLLMSVNPGFGGQSF
                     IEHVLPKIEAVRKRIDATGRDIWLEVDGGVKADNARRVGAAGADTLVAGSAVFSGGRY
                     KESIEAIRTNALAGRHSRQAKDPV"
     gene            41756..42472
                     /locus_tag="PKV56_01880"
     CDS             41756..42472
                     /locus_tag="PKV56_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX66585.1"
                     /translation="MKPDLSCIAAIAFDLDGTLVDSAPDIGHALNTALADGGLSCFDL
                     ATVRTWIGDGPDALIARALAAQDVSPDDAGLRQRLRRGFDEATLAAPLDNGCVYPGIA
                     DLLDSLHGVLPMAVVTNKPTRLAQAVLEAAGLSHHLMHVQGADTPAQRKPAPSMLLST
                     AARFGLQPSRMLMVGDAPPDMLAAQAAGCLAALVGWGYGAHAVPSASRPWRVETPQKL
                     GSSLLAMAHPSRPSPTVSID"
     gene            42483..43574
                     /locus_tag="PKV56_01885"
     CDS             42483..43574
                     /locus_tag="PKV56_01885"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOX66586.1"
                     /translation="MPTGGKSTLTQFLIEERRRAPEASGDLNALITAVSLACKAISRR
                     VAYGALATGSDAATAASQAGNLNVQGEEQKPLDILSNQMFLRANEWGGHVAGMVSEEM
                     EEMYTLPAGYPRGKYLLLFDPLDGSSNIDVNVAVGSIFSVLRAVTPEQDPKTGDFLQP
                     GTRQVCAGYAIYGPSTMLVLSMGRGTHAFTLDPLLGEWVLSHPNLQIPKKTREFAINA
                     SNSRFWEPAVKRYVDECLAGKIGPRGADFNMRWIASLVAETHRILMRGGVFMYPRDTK
                     DPSKSGRLRLLYEANPISMLIEQAGGSASTGRTRLLDVQPESLHQRIGFVFGSSDEVS
                     RIETYHREEPPETYSSPLFGKRGLFAAAD"
     gene            43610..44488
                     /locus_tag="PKV56_01890"
     CDS             43610..44488
                     /locus_tag="PKV56_01890"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOX66587.1"
                     /translation="MSAKHPVIAITGSSGAGTTSVTRTFENIFRREDVQAAIVEGDSF
                     HRYDRKSMKAAMAEAEAAGNMHFSHFGAAANLFEELEALFRDYSATGTGQSRKYLHNA
                     EEAAPFGQEPGTFTPWNPLPTSELLFYEGLHGGVSTDTVDVARHVDLLIGVVPVVNLE
                     WIQKIHRDRSTRGYSTEAVTDVILRRMHEYVHFICPQFTRTHINFQRVPVVDTSDPFI
                     SRTIPTADESLVVIRFANPRGFDFPYLLSMLHDSFMSRANTLVVPGGKMELAMQLIFT
                     PMIMRLVERRRQLLAR"
     gene            44569..45426
                     /locus_tag="PKV56_01895"
     CDS             44569..45426
                     /locus_tag="PKV56_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66588.1"
                     /translation="MSTVRACPTCTQINPTGSVVCMACGAELSAAARRRPKATELSID
                     LGRLESEEDTVPAVHLDAPELQRWVDSGPDALETTCAVPELTLRSVDALPVLENVADA
                     AETLPRGVVARSDGEPAAGKASVAKAARRAAVRRNRLSGHRQAGAAATPTDVLVLGRD
                     EAAREALCGLLETFGFRAHPAQTPVQAVRLLDAKHFVAAFLDLVFDGPQQRASVDLCQ
                     RIKAEVHQAMGRASALIIVSSSSRPVERVRATLAGCDAFLVQPVTRGGVAQALEACSV
                     ALPADSRGN"
     gene            complement(45427..45642)
                     /locus_tag="PKV56_01900"
     CDS             complement(45427..45642)
                     /locus_tag="PKV56_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66589.1"
                     /translation="MWFPHWLYESLPLLYALTAAACLFALESSFGSVLSTTALAAAAV
                     SVWNMRRKARSAVLRKAHRPSSRQQPR"
     gene            complement(45811..46440)
                     /locus_tag="PKV56_01905"
     CDS             complement(45811..46440)
                     /locus_tag="PKV56_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX66590.1"
                     /translation="MIRVMLVDDHALVRMGFKMLLGDAQVEVVSEVGSGEQACLEYPK
                     VLPDVVVMDLSMPGMGGLEAVRRLLAHDPNARILALSAHEDTAHPRRVLRAGALGYLA
                     KRSAPEALIAAVTAVARGDRYVDAQTAQALAMAQIDGETSPADMLSEREFSVFIQLAQ
                     GRSVATIADSLSLSPSTVGTHLYNVKQKLNATNQSELTLIALRWGLIQT"
     gene            complement(46451..47827)
                     /locus_tag="PKV56_01910"
     CDS             complement(46451..47827)
                     /locus_tag="PKV56_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX66591.1"
                     /translation="MNLRVKINLIVGVLMLMFVAAVLGLQVRSLKESVAEEVVAANRV
                     ASQLLNRTLWLYAAQGLPAMRAFLQGMGRVRANDIELFDQGGRRQYASPPSPYKSGRE
                     APDWFVGLVAPPPSVQAIDFPGGKLVVRSDASRAVLDAWDYFVHLALIGMALLAAANV
                     LVFWLVGSTVRPFSQIVRALNDLQGGRFDVALPALPGTEAAAIGAAFNRMVGVLRDNI
                     ETERRAARAERQLSDNRELTRWVDHHIEKERLLIARELHDELGQSVTAIRSMALSIAQ
                     RARTRADPQSEQAAQLIAEESARLYDAMHGMIPRLTPMVLDNFGLAEALADLVERTRR
                     SQPQVQIELQVDLADTPLDTERALALYRSAQEGITNALRHGHATALALTVRRQADTVL
                     LRLADNGSGLPADWAQRVGHHGLRWLSERVEALGGVVEVGPAPSRGVLVQVQLPLPAA
                     QVAEAEHS"
     gene            complement(47824..48780)
                     /locus_tag="PKV56_01915"
     CDS             complement(47824..48780)
                     /locus_tag="PKV56_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX66592.1"
                     /translation="MLYPATPPLRTHALRVSDVHTLHVEECGNLRGIPAVVLHGGPGS
                     GMSPLLVRFLDPARYRIIGFDQRGAGRSTPRGATRDNTTADLLADLQAIRHHLDIERW
                     LVVGGSWGATLSLAHALAESHAVSALLLRATFLARAQDIAHFFQGAAADRPEAWTRFA
                     AIAPPARRHALLEVLTEGLQGDVAQAAPFALAWRRWEAAMSCAPLPPDEPEGDALQAL
                     IGRYRVQSHYLSHCCWLDTPPLLDRCAALPCVPTAILHGGSDHICPPDGARELHRRLP
                     HSTLRVIPGVGHDPTHPEMVAAMLRALDSYAASGRFEPEVNS"
     gene            49186..50106
                     /gene="pqqB"
                     /locus_tag="PKV56_01920"
     CDS             49186..50106
                     /gene="pqqB"
                     /locus_tag="PKV56_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HOX66593.1"
                     /translation="MRITVLGSAAGGGFPQWNCNCANCAGIRAGTLRGKPRTQSSIFV
                     RPDDSADGVLFNASPDILEQIRNSPVMQPARHLRDSAIAGVVLMDGQIDHATGLFMLR
                     ERNSKLPLWCTDPVAEDLTQGNPVLRVLTHFCGVDRHAITLDGSSFEVPGVTGLSFRA
                     LPLSSKAAPYSPHRDNPVPGDNIGIVITDQRSGKTAFYAPGLGEITTPVFAEMSRADA
                     VLVDGTFWTDDEMVTLGISKKRSREIGHLPQSGEGGMIEWMSKLPPKTRRLLIHINNT
                     NPILDEDSTQRAELTRAGIELCEDGMLIQP"
     gene            50138..50896
                     /gene="pqqC"
                     /locus_tag="PKV56_01925"
     CDS             50138..50896
                     /gene="pqqC"
                     /locus_tag="PKV56_01925"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HOX66594.1"
                     /translation="MIQESRHPDAARSGQADDNAAWSREAFEAKLRERGKSYHIHHPF
                     NVMLNTGKATPEQVRGWVANRFYYQIAIPIKDAAVLSNCPDQAVRRGWIQRILDHDGF
                     EIGGIKDPGGIEAWLRLAQAVGLTREEVQDLRHVVPAVRFAVDAYVNFARRAPWQESV
                     CSSLTELFAPEIHKQRLATWPEHYHWIESEGLSYFRNRVGQARRDVEEGLAITLNHFT
                     TRPLQERALEILQFKLDILWTMNDAMATRYGVSS"
     gene            50893..51192
                     /gene="pqqD"
                     /locus_tag="PKV56_01930"
     CDS             50893..51192
                     /gene="pqqD"
                     /locus_tag="PKV56_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HOX66595.1"
                     /translation="MTTSTVAQGSLLSNTVRPTVGPGFRLQWEPVQNCHVMLYPEGMV
                     KLSQSAGEIMKRCDGQRSVAEIVSDLEQAFSTQGLEGDVVAFIEMAGKNRWLVWS"
     gene            51189..52349
                     /gene="pqqE"
                     /locus_tag="PKV56_01935"
     CDS             51189..52349
                     /gene="pqqE"
                     /locus_tag="PKV56_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HOX66596.1"
                     /translation="MSGPVMSAAPRPGPPLWLLAELTYRCPLHCVFCYNPVDFARKED
                     ELSTEDWFRVLQQGRELGAVQCGLSGGEPLVRDDLELIVAEAHRLGYYTNLLTSGVGL
                     TAERASRLKAAGLDHVQLSFQDSTKQMNDFLSHTKTFDLKNRVGKIIKDQGWPMVMNV
                     VIHRMNIDHIDRIIGMAHEMGAEYLELANTQYYSWAMVNRDHLLPTHAQLKHAEQVTD
                     AWRAKLGNAMRLFFVAPDYHEGKAKKCVNGWGSMFLTVVPDGTAVPCHTAQMLPGLDF
                     PNVKEHSLSEIWFDSEGFNRYRGTGWMKEPCSSCEFKEIDLGGCRCQAYMLTQDPTAT
                     DPVCIKSPHHHLVTDAVAQADVVAANPVTEHPLVFRDRVNSLRLIETESPHC"
     gene            52522..53022
                     /gene="fae"
                     /locus_tag="PKV56_01940"
     CDS             52522..53022
                     /gene="fae"
                     /locus_tag="PKV56_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOX66597.1"
                     /translation="MTNVRVGESLVGEGNEVAHIDLLVGPRGSAAETAFANALVNNKD
                     GFTSLLAVVAPNLATKPNAVMFNKVTIKGAKQAVQMFGPAQRGVAMAIADCLAEGTLR
                     ADQANDLFISVGVFIHWMAEDDKKIQEYNYRATKESIERAVSGFPTAAEVQAQYKTAK
                     HPFGAD"
     gene            complement(53084..53629)
                     /locus_tag="PKV56_01945"
     CDS             complement(53084..53629)
                     /locus_tag="PKV56_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOX66598.1"
                     /translation="MRPDLLRHCGAAILFACWLMLAHGGARSAGLPVPYRAIVVQPTG
                     LPTQAEFDLRPAIDQARREKKSLYIYLGADDCPYCRRYEAFLAQNAGELLPHFAGYLI
                     VDLRSSLKISSKALIIRTNLGALPYAEFQRTIGDERARLLVYPSVWLLDTEVKPLMQM
                     PAGTGTFQTVPEQLEILNLVQ"
     gene            complement(53619..54509)
                     /locus_tag="PKV56_01950"
     CDS             complement(53619..54509)
                     /locus_tag="PKV56_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HOX66599.1"
                     /translation="MSKSARLWRALWMVCVLASVGWIAGPAQAAVGGLNSNAAHNDSS
                     PEWEKLRARFFPNRSIAADAASQVQLIVPLRAAYGASVPVKVVSKLPQRNELYVKKLY
                     LFIDKNPSPLAAVLDLTADLGQADFETRLRVDEYSHVRVVSELSNGELHMDSRYVKVS
                     GGCSAPPNREHPELIGKTILKLNGPVVYDRPNVADVTVLHPNDTGFELNNITVMFIPP
                     HFIRSIKVRYGEHKLFDADLDFSVSENPTFRFNFVPRGDATLTAEIEDSREGRFTGML
                     PVHASGLPGQGDAPVDSRAP"
     gene            complement(54532..55518)
                     /locus_tag="PKV56_01955"
     CDS             complement(54532..55518)
                     /locus_tag="PKV56_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX66600.1"
                     /translation="MSLLRRRGRISFACAAFGLAMLAGCATGGRFLAAEPDGQVAGGT
                     TVQVADGVYMVRGTGGEVDSSNLGRIGNAGFIVGDRGVIVIDVGTSHRHGDELLEAIR
                     RVTDKPVVLALVTHVRQEFLFGAAAFRDRGIPIRMQRSAADLMAARCANCLKTLRRLL
                     GEDEMRDTAMFKPDQVFDDSHTLDVAGRPVRVMYFGHSSGPGDIAVLDERTGVLFAGG
                     LMDQQRIPDIQDSQLPGWRNALAAMDALRVSTVVPGHGRATSSGLITTTRRYLDTLEA
                     RVRQLVMDGAALSEVPDASSLDEFKEWDQYDTIHRRNASVLFVRVEREVNFK"
     gene            complement(55515..56063)
                     /locus_tag="PKV56_01960"
     CDS             complement(55515..56063)
                     /locus_tag="PKV56_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOX66601.1"
                     /translation="MLRTKLNANPVRWMAWVVLFGLMSVAQAHGPTRQKVSEKITINA
                     PAAAVWARVKNFDALKDWHPAVAESPASAGNTEGSVRALKLKGGGALEETLESFDEAK
                     MKFSYRAKDGGALPVTNYTSTLQVTSAGAAQSIVEWRGAFYRAFPNNDPPPDQNDEAA
                     LKAVTGVYKSGLENLKKLVEGQ"
     gene            complement(56111..57106)
                     /locus_tag="PKV56_01965"
     CDS             complement(56111..57106)
                     /locus_tag="PKV56_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOX66602.1"
                     /translation="MSAKLHWAAARAMAFMLLLGATVASSHAAPFAYITNQGSHDVSV
                     IDLLIDSVVATVPVGRGPAGVAIAASQAKVFVTNPDSKTISVIDMRSRQVVDTLAAGD
                     GPLGIAASDDGMRVYVSDWYRNRVLVFDSRVHHPLMSIAVGRAPAGLAVHESGEAVFV
                     AERDDDSVAVIDVNSQRVLSRVTVGARPFALLYDTPRQRLYALNVQSNDLSVLDTRDL
                     FRTGKLVPIATVAVGKAPYGAAMADGGKLLYVTNQHEDSVSVIDADTLKVVRTLRGFG
                     YPEGIASHRDRVYVVNWMDDNLSVLDAVSGQTIKTIATGQNSRGFGAFIGDAPAP"
     gene            complement(57103..58050)
                     /locus_tag="PKV56_01970"
     CDS             complement(57103..58050)
                     /locus_tag="PKV56_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HOX66603.1"
                     /translation="MIRWWHALLLWAAATSLACAQAALPPLQVAELSPGVFVHSGAVA
                     DWNSASAGDVANLAFIVGTRCIAVIDSGGTPDVGRRWQAAVAAISPLPVCHVINTHAH
                     PDHVLGNNAFRTADSAAGPGFVTHARMRAALAARERYYLNALQRDFGVAMKHDDLVYP
                     TVTIAVGQTLEIDLGQRVIEVKAWPTAHTDHDLTVFDRQTRTLFLGDLLFVTHLPVID
                     GSLLGWIAAMDRLAQMPVALAVPGHGAAGRDWPSMLGPQRRYLDALLTETRAAIRAHS
                     TMQGAVRTVGRDALQGWALADHFHERNVTTAFAELEWEE"
     gene            complement(58112..58480)
                     /locus_tag="PKV56_01975"
     CDS             complement(58112..58480)
                     /locus_tag="PKV56_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66604.1"
                     /translation="MTVSRNPARVATLLLCASFSAVLQANDFPTLDRVIYVRDCMREN
                     PGPQFEMISKCSCALDRMAEQVKYDEYVTLSTDANATSIGGERGSAMRDNEGVQKEAK
                     RFRELQAKVKNSCFIGTGPK"
     gene            complement(58490..59212)
                     /locus_tag="PKV56_01980"
     CDS             complement(58490..59212)
                     /locus_tag="PKV56_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66605.1"
                     /translation="MQFMKFPRFAGACLALLMAFASTSRAQDKPPENKQDFSPAEKLL
                     FMSNQLSGVKPATTIRYSFRKAGSFEEGFEDSVVLSFEKQADGTCCTVKSDFFSGTRR
                     LPLPEAPMAEGNPVLLHFLERDIGEMKRLTKGSLNHFRKRIRMAAYQDATVRDTSLSY
                     RNKTIAGKEITFTPYLDDPNRPKYEKFVRKQYQFLLSEAVPGGIFGIRTRISGETSGL
                     PDVIVEELFVEGASAVIPTQIK"
     gene            complement(59240..60253)
                     /locus_tag="PKV56_01985"
     CDS             complement(59240..60253)
                     /locus_tag="PKV56_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX66606.1"
                     /translation="MKYAALWLAVACSWGTAQAGVDRAAFIELSTKVLKVEVSREQGG
                     YSLGSGVLVAPQRLLTNCHVTRDAHQIHAYQYGVRMQAVAQVSDVRRDLCLLIVPGLS
                     GDGVVLGSSGSISEGQSVSAIGYTGGMGIQNSSGEVVVLHPFDGGRVIQSTNWFTSGA
                     SGGGLFDDDLRLVGILTFRLRGGEAHYFSAPADWVKDLLARQADAREIASIPATELAY
                     WQQPLTQQPRFLQAAVLERDSRWDDLEHLASGWTAMHEHESEPWYLRGLALQKLNRLT
                     DARDALERSVNIAPTLALAWHRLGLVYSGLGMTAQAQSVQRRLRQLNPDLALQLAKSL
                     LPN"
     gene            complement(60290..61132)
                     /locus_tag="PKV56_01990"
     CDS             complement(60290..61132)
                     /locus_tag="PKV56_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX66607.1"
                     /translation="MCITSALGLGWSPYATAQDEAKPSRQAFRVCQDPNNLPFSNVGG
                     DGIENRIAELFAKELGLPVTYYSFPQRLAFIRNTLRYKLPGEDFRCDIVMGVPAGYDQ
                     VSATKPYYHSTYALVIAKGRGLDQVKSVEDFLAVDRTRLGKLKIGLYDRTPASDWLVK
                     HGLLEQGVPYATMNAAPEFYPGEIIERDLASGKIDAAIVWGPIAGFFAKRVKSPELMT
                     FPMRSEPGVKFDFKMAMGVRYGEREWKQQIESLIDAKRPEIRAILEEFGVPLIEDTPQ
                     ALTN"
     gene            complement(61192..61530)
                     /locus_tag="PKV56_01995"
     CDS             complement(61192..61530)
                     /locus_tag="PKV56_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX66608.1"
                     /translation="MAQEVKTDLYTVVDGNKVDAQTLKGFKTWRTAACDRCHGANQEG
                     LVGPSLVNSLKVLTKEEFVTTVTNGRLEKGMQSFGNSPVVMENIDHLYAYLKGRSNGD
                     IKSARVTAIE"
     gene            complement(61711..>62027)
                     /locus_tag="PKV56_02000"
     CDS             complement(61711..>62027)
                     /locus_tag="PKV56_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HOX66609.1"
                     /translation="LNTAGGLSCYGTLEGYFKCVDKKDVNKELFKFKTPSGIIGNVFT
                     YEHKGKQYMGVFSGIGGWAGIGMAAGLEKDQDGLGAVGGYRELGQYTELGGSLTVFAL
                     PN"
BASE COUNT        10603 a        20474 c        20528 g        10422 t
ORIGIN      
        1 tgtgctgttg cagtcgggcc agggtggccc gcttgctcga tgcgagccgg atcgcctcgc
       61 cggtgcagcc tagccatcgc tgtggcggca ccacctcgtt cagtgccgcg agcagaccgt
      121 cggtctcggg agcgaccacc cacacgaggt catgcaaatc cgcctgtcgg gcgacgaagt
      181 cgagcgcgga ctccccgacg agcgcgcaga cctgccgggt cgaggcggga ccgtcggcac
      241 cgaaagatga atccgatcgg ccgacggcga cactgagctc gacgttcggc agggcggcca
      301 gatcagcctg catcgcatcg cgcatcgcga ggccttcggc gtgcaagccg gcagccgagc
      361 catggcagcg gtcgggcgtg ccggcgctga gatactcgaa caggaacacg cgccgcgttg
      421 tcattttccg tggatgcttc ctgggatccg ccgccccacc atgaacctga ttccggtgat
      481 cgatctgatg cgcggccagg tggtgcgtgc gcaccgcggt gcgcgcggca gctaccgacc
      541 gatcgaatcg aggctgtgcg ccagcagcga tcctattacc gtggcccggg tgctgcgcga
      601 ccattgcgat gcggatcagt tgtacatcgc cgatctcgat gcactcaccg gtggccggcc
      661 ccaggtggcg attctgagat cgctccagcg tgcgttgccg cagaccgaac tctgggtcga
      721 tgcgggctgt ggcgatgcca gggtcgccaa cgagttgctg gaggccgtcg atgcgcctgc
      781 gggctgcctg attcccgtgc tggccagtga gtcgatgcgc tcacgccagg cctttgaaca
      841 ggcctttggc ccggcgggtg acgacgggtc gcacgcgcgc aaggactggg tgctgtcgct
      901 cgaccgccgg gacggcgtgc gcctcgatgc ggcagggtgc tggacgctgc cagcgctgtg
      961 gccgacgcgc gtcatcgcga tgacgctgga gcaggtgggc accgatgccg gccccgacct
     1021 cgaaaccttc gctgcgttga gtcagttgaa tgcctcggtc cgctggttcg gagccggcgg
     1081 cgtgcgagac ccgggggacc tcgagcgcgc gcgcgccgcc ggcgccagcg gctggctggt
     1141 cgcctcggcg ctgcacgacc tgcggctgcc cccgatgagg cgctgacctt cgcccgctgg
     1201 catccacaca caatgtcacc accgcacggc acaaagtgtt gcccttgatg gcgcgtgttc
     1261 ctgcccggtg agcttgtgcg ccaggattcg cccggcgtgg ggttccccgc aggcaattgg
     1321 cctgtccctt gcgacacaat gatttcatga gcgcttctgt gtcaacggct acatccggca
     1381 gcgggtccga cacggcctgg acctgtccat tctgtccgct gctgtgcgac agctttcaag
     1441 tcgcgatccc ggccgatcag gctgcgctgg cgttgcgggg cagtgactgt ccccgggccg
     1501 gccgcgcgct gcagcgattc agcggtgccc cgtccgttgc ctcagcacag atcgacggaa
     1561 agtcctgtga tctcgacagt gcggtggcgg cggcagcgca ggtgctcgcg acgagtcgcc
     1621 agccgctgtt cggcgggctc ggcaccgatg tcgccggtgc gcgcgcgctc taccgcctgg
     1681 cctgcgagac cggtgcgatc tgcgatgcgg cgcaaggcga tgcgttgatg catggattgc
     1741 gcgcgctgca ggaccgggga cagttcacga gcaccttcgc agagttgcgg actcgagccg
     1801 atctgatcgt ctgcctgggc ggatcgccgg ccgtccagca cccggagttc ttccgccgct
     1861 gcggtgtcgg cgaagatctg gtcggtgcgc gtcacatcgt tttggtcggg gcggcagccg
     1921 gtgacgacgt gccggccacc ctggcgaagc tgaacggcgc tcgcggcgtc accgccgagg
     1981 ccatcgatct ccacggagac ctgttcgaca cggccgcgat gctggcagcc ctcgtcgcga
     2041 atcatgcggt gagcgcggca ccggccgctc tcgtcgcgct ggcccggcgc ctgcacgccg
     2101 cgcaatacgc agtcgtcgtg tggcagaacc cggagctgcc tgcgcatggt gcgttgctcg
     2161 tcgagaccgt caaccacatc gtcggcctgc tcaatcgcag cacgcgtgct gcggggttcc
     2221 cgctcggcgg cggcaacggt gcagccacgg tgaaccaggt cttcagctgg ctgtccgggc
     2281 tgccgctgcg ctcgcgggtg ggcccgaccg ggatcgagca cgagccgctt tgcttcgacg
     2341 cccgacggct gctggccgac ggtgcggcag atgcgctgct gtggctgtcg agcttcaacg
     2401 cggatgcgtt gccctccgcc gatctgcctc gcgtcgtcat cgggcatccg gacctcatgc
     2461 agcatctttc cgcctctggc gcagggcacc gaaccgtgtt cattcctgtc tcgacacccg
     2521 gcatcgggag cagcgggcat cttttccgcg ccgacggtgt cgtgctgatg ccgctggatg
     2581 cggtctatgc cgacaccttg ccgacggccg ccagcgtgat ctggcgcctg acaacggccg
     2641 tcgctgcgtt gaagcagggg gctgcggcat gaccacggtg cgattgaagg gcggccgggt
     2701 catcgacccg acgcatggcg tcgacgacgt ggtgcgcgat gtctgcatcc gcgacggccg
     2761 catcgtcgag ttggcgccga acgaggccgt gaccaccgag atcgacgcca gcggctgcgt
     2821 cgtgatggcc ggtggcatcg acatgcacag ccacatcggc ggcggcaagg tcaacctggc
     2881 gcgcatgctg ctgcccgagg atcaccgcgc ccgcggcaac ccctacgcgc tgcccgagaa
     2941 ccggctcgaa ctggcctcgt gcggcacctg tgcgccgggc acgctggcaa ccggctaccg
     3001 ctatgtcgag atgggctaca ccgcggcgtt cgagccggcg atggtgtcgg ccaatgcgcg
     3061 gcatgcccac atggaaatgg gcgacgtgcc gatcctcgac cacggcgcct acgtgatgct
     3121 cggcaacgac gagctgttcc tgcagatgct ggccgagggc cgcgacttcc agcagatccg
     3181 cgattacatg ggctggacga ttcacgcctc gaaggcgatg ggcgtcaagg tcgtgaaccc
     3241 cgccggcatc tcggccttca agttcaacca gcgcaagctt gacgtcgacg agaagcatgt
     3301 gcactggcag gtcacgccgc ggcaggtcgt gaataccttg gccagagcac tgggcgagct
     3361 cggcgtgccg cacccgctgc acatccacgg cagcaacctc ggcgtggcgg gcaacatcga
     3421 gtcgacgctg gagaccattt cggcgcttga cggcctgccg ggccatctga cgcacatcca
     3481 gttccacgcc tacggcaccg aggggccgaa gaagttttcg tcggcggcgg tgcgattggc
     3541 cgaggcggtg aacgcaaacc agaacatcag catcgacatc ggccagatca tgttcggcca
     3601 gaccgtcacc gcgtcgggcg acacgatgcg gcagcatgcc aattcgggca tcggtacgcc
     3661 gcgcaagtgg ataggcgccg acatcgaatg cgatgctggc tgcggtgtcg tgccgttccg
     3721 gtaccgggag cagagctatg tgaatgcgct gcagtggtca ttggggctgg agatcttcct
     3781 gctggttgac gatccctggc gcgtcgtgct cacgaccgac cacccgaacg gcgggccgtt
     3841 caccagctac ccccacctga tccgcctgct gatggaccgc agcttccgcg acgagcaggt
     3901 cgcgaagctg caccccgaca ttgcggccaa ctcggcgctg cgttcgatca cccgagagct
     3961 gtcgttgtac gagatcgcgg tgctgacgcg tgccggcccg gcgcggctgc tcggcctgcg
     4021 cgaccgcggg cacctcggag cgggtgcggc cgccgacatc gcggtctacc gcgacgccgc
     4081 cgaccgcgag gccatgttca ccacacccga gtacgtgttc aaggacggca cgctggtgtc
     4141 gcgcgccggg cgcatcgtcg cggtgccgac cggcggcacg cacttcgtcg aacccgacta
     4201 cgaccgcggc atcgagaaga cgctgcgcaa gcacctcgcg gcacacggtt cggtgaactt
     4261 cgaccacctg acgatcagcc acgacgagct gtgcagttgc tgcaacggcg ggcgcctgct
     4321 gcccaccgag tgcttcgagg gagcgacgac gtgagcgcgg cttcagcaga catcgcgaag
     4381 gcgccgctgg tcatcaatgg cgtggtgatc gatgacacct tcgccgaagc gttcccgatg
     4441 aaggcgacgc gcatcgtgat caccgcgcac aacctgacct gggcgcgcca cgccgccgtg
     4501 gcgaccaccg gcttcgcgac ctcggtgatt gcctgtggct gcgaggccgc catcgagcgc
     4561 gaactcgacg ccagcgagac tcccgacggc cgtccgggca tcagcgtcat gctgttctcg
     4621 atgtcgggca aggagctggc gaagcagctc gaacggcggg tcgggcagtg cgtgctgacc
     4681 tgcccgacga ccgcggtgtt tgccggcatt gccgacgggg ccgacgtgat cgaccgcatc
     4741 gcgctcggca agaacctgcg cttcttcgga gacggctggc agatctcgaa aatgattggc
     4801 ggccagcgct actggcgcat tcccgtgatg gacggcgagt tcgtcgcgca ggaaacggtc
     4861 tgcgtgacca aggcggtcgg cgggggcaat ctgctgctgc tggcgcgcga taccgattcg
     4921 gcgctcgccg tcgccgaggc ggcggtgctc gcgatgaagc gggtgcccga tgtgatcatg
     4981 ccgttccccg gtggcgtggt gcgctcgggc tcgaaagttg gcagcaagta cccgtcgctc
     5041 ggtgcatcga ccaacgacgc gttctgcccg agcctgatcg gacttgccgc gaagaccgag
     5101 ctgaccccgg aaacccgctg cgtgatggag atcgtgatcg acggcctgac cgatacagcc
     5161 gtcggcgcgg caatgcgtgc cggcatcgat gccggcaccc acatcggcgc ggtcgggggc
     5221 ctgctgcgga tctcggccgg caactacggc ggcaagctcg ggccgtacca ttttcatctg
     5281 cacaagctgc tggggcaggg cgcacagggc ggccagtcat gagcaccacc atcaccctga
     5341 ccctgaagca ggcgcccgcg ctgcgggttg atctgcgcgg tgtcacgccg gcggcgctgg
     5401 ctgcgttgtc ggtgggcgcg gtcgaacggc tgtccgtcac gcacggcacc cagatacttc
     5461 cactggccga gctgttcggc gttgttcgat ccgattcggc tggcgatgtc gatgagctgc
     5521 gtctgatcgg ggatctggcg cacttcgacc gcgtcggctg gcagatggcg ggcggccgcg
     5581 tgttcgtcga tggcagcgtc ggcgactacg tcggcggcgg catgagcgct ggcgagttgc
     5641 aggtggccgg cagcgccggt tccctggccg cctgcgagat ggcgggcggc ctgttgacgg
     5701 tgactggaga cgtcggcgac ttcgccgcca gcaccttgcc cggcagcatg gacggcatgc
     5761 gcggcggcac cttcatcgtc aagggaaatg cgggcgcacg ctttggagat cggatgcggc
     5821 gcggcaacgc gctggtgttc ggcaacgcgg gtgactttct cgcgtcgcgc atggtcgccg
     5881 gcacgatcgc acttggcggt gaagcgggcc cccatgccgg ctatggcatg cggcgcggca
     5941 gcgtggtttt cgcagcctcg aatgctgcag gtgacatcgc ccagaccttc gttcctgcgg
     6001 tggccgacgc gccggtgttc tggcagctgc tctcgcgaga cctcgcccgt cacggtgggc
     6061 cgttcgccgg cctgccggca cgctcgatcc gccggtatct cggcgatgtc gccgcgggcg
     6121 gcaagggtga actgatcgtc gccggctgag cccccgggcg ggcgggtacg cttcgtatcg
     6181 cgccaactcg tcccaacgtt caccggggta cgaggtggtc acagtttcaa atcaactttc
     6241 ggattcgaca gccatgagca agaactacat ctttcacgcg ggtgaggcca cggtgctggc
     6301 caaggacggc cagttcaccg acgcgatgcc cgagatcctg atcggctcta cctccggccc
     6361 ggtcgggcac gccttcgcga acatgatggc gcagagcaag gggcacaccg cgatgttcgc
     6421 gatccgcgcc tgcaaccagc tcgtgcgccc ggcgaccatc gtcgtgccca aggtcacgtt
     6481 gaaggacagc gccaacatcg acctgttcgg cggcgtcgtg cagagcgcga cagccgatgc
     6541 ggtggtcgat tgcctgatcg acggcacgat cccgaaggac attgccaacg aactgtgcat
     6601 catcagcctg gtgtggatcg acccgcgttg cgcgaaggat ccgaacctcg atcgcaggga
     6661 catgtaccgc accaaccacg aggcgacgat gctggccatc aagcgggcct tgaacaacga
     6721 gccgtcggtc gatgagctga tcgccaatcg ccacaagatc aagcacgaca tggacgactg
     6781 gagctgacgt cgtgaatcct tctgctgcgc gggtcgtcga tctggcgttt ccggtgctca
     6841 ccgcacctgg gtacggctcc acgcccgtag gtgccaactg cggccacctc ggcccgctcg
     6901 cgacgaaacc ttcacgacgt caagcgctgt tgatgtgatg gatcgctgct gcgcgctcct
     6961 cgacgaccgc gacgcgacgc ccgagcggcc gaccagccgg ctgtacaccg ctttcgtgcg
     7021 cgagcaccgc tgcatcgatc cggccacgct cgacgctaca tgggcgcaag tcgaggccga
     7081 cctgcgaagc ggactgcatg ccctggtgct gggcgactac gaatggggcg tcaagttgct
     7141 gaaggccgga catcgcacgc tggtgcccgg cgaccggtct gcgctgcggg tactgatgtt
     7201 cgccgacctg gcgctcctgt cgcgcgccga agcggacgaa tggctggagc gtctcgaggc
     7261 cgccggtgac gttgaaacgg tcgtgcccgg cgccgccggt gtcatgcacc tcgaggcgag
     7321 cgtcgaccag ccggccttca cgcgcgcgat cgatcgcatc cacgaggcca tccaggccgg
     7381 cgagacctac caggtcaact acacctatcg cctgcacggt caggcctacg gctcgccggt
     7441 cgggttgtac cgtcgcctgc gtgcgcgtca gcccgtggcg ttcggcgcct acatcgaact
     7501 gccgacatcc gacgcgcacg acgacgcaac gacccatgtg ctgtcgtgct cgcccgaact
     7561 gttcctgcgc aacgaggctg gcctgctgac agcgcgtccg atgaagggca ccgcctctcg
     7621 tatacgtgca cccgaggccg acagtgaaac cgcgcgccac ctgtctctcg acgtcaagaa
     7681 ccgcgccgag aacctgatga tcgtcgacct gctgcgcaac gacataggcc gggtggcaca
     7741 gatcggttcg gtcacggtgc ccgatctgtt cgcgatcgaa ccctactcga cggtgttcca
     7801 gatgacgtcg accgtgcagg cgcgggtgcg gcccgacgtc ggtttcccgg agttgttgcg
     7861 cgcggtcttc ccttgcggat cgatcaccgg cgcaccgaag catcacacga tggagctgat
     7921 cgccgagctg gagacgactc cgcggggcct gtactgcggt gcgatcggct gggtcgacgc
     7981 gccgcgcgcc gatgcgcggc tgggggactt ctgcctttcg gtgaccatac gcacgatcac
     8041 gctcggcgcc gacgtggccg gcctgcgccc cgcccgtctc ggcatcggcg ccggcatcgt
     8101 gctcgacagc cgagccgaag acgagttcga cgagtgtcgg ttgaaggcac gcttcctgac
     8161 cgggctgccg ccggggttcg acttgttcga gacgatgctg gcgactgcgg agaccggggt
     8221 ccgccacctc gaccgacatc tcgcgcgact cggccgcagc gcacgcgcgc tcggattcgt
     8281 attcgatccc gtcgaggcgc agcgactgct cgatgcaagg ttgggtgaac tctgcgccag
     8341 ggaggcggcg cgccatcgcc tgcgcttgtc gctggcccac gatggtcgcc tgcagctgac
     8401 ccacgccatg ctcaccgatc tgccacctgc ggcggatggg gtgacgctgc tggtggcgga
     8461 tcatcggctg ccgaactcgc atccgttgtc cggccacaag accacgctga ggcagcatta
     8521 cgacgaaggc gttcgcgccg ccgagcgggc cggcgccttc gacagcttgt tcttcaccga
     8581 ggacggacgg ctcgtcgagg ggggacgcac atcggtgttc ctgaagctgg ctggccgctg
     8641 gtgcaccccg ccggttgctg acggggcgct gccgggcgtg atgcggggcc tcgtgctcga
     8701 agatcccgcc tggcaggcgg tcgagcgcag tctcgcacgc gacgatctcg accgtgccga
     8761 agcggtgatg gtttgcaacg cgctgcgcgg ggtgttgccg gcccgcgtga tggccacgcc
     8821 ggacaggtct gccgctgcat gaacctggcg ctgttcgacc tcgaccacac gctgatcccg
     8881 ttcgacagcg gcatggcctg gactcaattc ctgattgcca agggtgtgct gccatcgacg
     8941 gctgcagaca cttacctcgg ctactgtcat cagtacgtgg ccggaacgct cgacatccac
     9001 gacatgcacc gtgccaatgt ctcgccgctt gcacggtatc cgatggcgca gatccgccag
     9061 tggggagatg agttcgaggc gcacctcgcc tcgcgcgtgc cggccccgat gctggctctg
     9121 gtgcagtcgc atcgagatgc cggccacctg tgtgccctgg tgaccgcgac gacccgcttc
     9181 attgcggagc cgctcggccg actcttcggg ttaccgcatg tcgtggcgac cgaaccggtg
     9241 atcgtcggcg tggctcacga cggacggctg accggtgaga tcagcggatt gccttgtttc
     9301 cagcagcaca aggtcacgcg agtcgagcaa tggctgaccg ccgggtccgg cgctgcaggc
     9361 tggcatccag acttgcgttc attcgagcga tcctggttct attcggactc ggccaccgat
     9421 ctgccgctgc tgcaggcggt gactgatccg gtggcagtca ccccggatgc ccgtttgcgg
     9481 gcggtggcca tcgaagcagg ctggcccatc ctctgaccag ccacctgaca ggccgaagac
     9541 gggccccctc cggattcgca gggcccgggg tcctcagcga accacgatag gcagcgaggc
     9601 gaccggcacg acctcgggcg cagtggctcc ggttcccaga ggcgaactcg gcacggccgg
     9661 gtgtccgacg atgaatgtgt tggcgtccac cccgcgctcg gcacgtgccg agaacgccgg
     9721 atgttcgaag ttggcgtgcc ctgcgcggac cgacggtgag gccgggtgcc ggatcaggta
     9781 cgactgggtg acgacggggc cgacatcccg gaccatcgtc ggataagggc cggctccgag
     9841 cgtcagggtc tgcgcgccgg ctccggttgc gagcgtcaag gtcaacaggg acagtgcgac
     9901 aaggcgttgg tgagtcatgg tgtttaccct cgattgcgat gtccggactg taccgggatt
     9961 acccccatat atttaggttt gttcatgcat ataaaaacga ctttcgcgat caaaacacgt
    10021 cgacttatcg gatgaaatcc atataaatca acgacatgtg cttgcgcgag aggcggtatt
    10081 aaaggttttt atgaacatat aagccgtgcg gccgcgcagc cgccggctca cttgagctct
    10141 tcgaccttgg cgcgggtgat cgcaccgtcg gatcgaccct tcaggtacgc gtacaggtgg
    10201 tcgatgttct ccatcacgac ggggctcgcg ctgaagctcg gcatgccttt ctcgagtcgc
    10261 ccgttggtca cggtcgtcac gacctgttcc ttggtcaggg tcttgaagct ctcgatcagc
    10321 gaagggccga ccatgccttc ctggttggcg ccatggcatc ggtcgcaggc ggccgagcgc
    10381 caggtgcgaa atcccttcat cgtctgtgca tcgaccttgt agccgtcgac cacggtgtaa
    10441 agcgcagtgg caggtgccga ggctgcggtc tctggcgtgg tctgcgccgc agcgctgcca
    10501 gcgccgagcg ccgtcaggaa ggaaagcatc gcgccgaggg tctttttcat cacagtcctc
    10561 ttcaacaaca agcaacgggg gcagggttac gccctgcaag gaagaaacgc tggcgcccgg
    10621 tcgccgttga cgacgggggg gcgaatcggg acctgcgcga cccccgagag cgactcgatg
    10681 tgcgtaattg cctcacgagt gcggcgcatc ggccgatatc catctcagac cggcgacaag
    10741 tgtcaccggt catcaccgag acggtcacat tcgcagggag ccttcatgaa actcgagaac
    10801 atcgttttcg ccgttgccgc gaagatcgac atcgatcgct cctttgtctt cgagtacgtc
    10861 gtgtgccggc acatccagtc gctgctgctc aatcgcactg cgctgccatc actggccatc
    10921 gatgtcgagg gtctcgagta cgccgaatcg ccggtcctgc accagtgcat cgaccgcgcg
    10981 gcgcacctgt tcctggcgga tctggtgcga gccagcgaaa tgtgcgaggc tgccatcgca
    11041 cgctggcaac tgacgttcga gacctgagat tcggtggcgc gtcggccgca tcatccttcg
    11101 ttcgcgtcgt catcgtcgtc cggatcggga ggtcccgccc agacatgctg cggcgtgcct
    11161 ggccggtgac gcgagcgctt ggccgaagtg agccgaaggt cggcgagatc cagcagatcc
    11221 tgcgcccgcc agtcgtcgcc gctgggtcgc gccgacacgc cgatgcagac gtcgatcacg
    11281 ccgaatccgg gatggatctc gtgcacgcgt ccgaccaggc cgcgtatggc cgacgcgagt
    11341 tgcgcggccg tggccagcga cgtgccgggc agcagcatcg cgaactgatc gccgccgagg
    11401 cgagtcacga aatcgtgcgc acgggctgaa cgcctcagcg tctgggccag agcaaccagc
    11461 gcctggtcgc caccgtggcg gcccgcttcc aggttgatga ggaacaggtt gtcgaggtcg
    11521 atcagcagca gtgccagcgc gcgcttgtga gtgcgggctt cgtcggctgc ttcgctcagc
    11581 cggtcgagga agcaggctcg attcggcaga ccggtgagcg tgtcgaggaa cagcgttgat
    11641 ccgttcatca tgagccgact ctacaactgc cgctgtcgtt tcagaaccgg tctgcgagat
    11701 ctcccttggc gcggggctcg gcgccagacg ccgcaggtga ccccaggcgc cgcgcctttt
    11761 cgacatcggt ataggacagc gttgcatctc cgatggcagg ttgccggtag atgagcttca
    11821 tgcctccggc gaaatcccag tcgtaggtgc cgcgtggcgc gaatctgccg gcaaagctcg
    11881 ggaaggggcg ccgggcaccg tcggccgtgg tcggcaagcc gtacttctgc accagcaact
    11941 gccgcagggc ctcgttctcc tgcccgtagg acttgatgat gaactgcgcg ctgacgaagc
    12001 caccctgatc ggcgatcaag gtcaggcgct gcagtgcggg cacaccggca tcccgggtgt
    12061 cgaaggccac ggggccttcg gggcgacgtt cgagttgcct tgcgccggcc gccgtggctg
    12121 cggaaacgaa cgcagccacc gatgcgtcct tcagccgcac gccgtagagg gtcagctcgc
    12181 cggagcccgg ggcgacctcc gcagcggaca cgggaggcag ccaccacagc atggccagga
    12241 gagtcatcgc cagcgggcgc gcgattcgag agagggggtg aacgtgaagc atcgccggag
    12301 tttcgcaggg acgggcttga ggccggaccc cgggctgccg ataacccggg ttggcccgca
    12361 tgtcgtctgt tctcagcccg tacctttcca catggcctgc tctgtcgatt gccccacccc
    12421 cttcacgatg aatgccgtca gatgaggttg aacctgcccc tgggacgcaa gcgggtcgct
    12481 ggcaccggat tcgatctcgg cgcggagaag tcgcgcgtgg ccgaggcgct ggtgcgaagt
    12541 gcgtcccgga tcctcgaaag tcgcgacgac cgggacatca tccacagcgt ctgcgaggcc
    12601 ctgaccctgg cgactccgca tgtgcggctc gcctggacct ggttcgggcc gctcgacacg
    12661 cctcgcatcg tgccgcaggt cgttgtcggg ccggcggcgg actacgcgcg caagctgtcg
    12721 atcgaccgca gcttcttcac cgaaatcggc tcggcgttcc ggtcgctggt cggcaggaag
    12781 gccgaagcgt tcaacgtctc ccggatgtcg ctctacggtc cctggcgcga ggcggcagcc
    12841 acccatggca tccgctcgtc gctcgccttg ccgctcgcct ccaccgtcaa tgaagaccgc
    12901 ggattgctgg cgctctacgc cgatcaaccc gactacttcg agcaggtcgg catcggcctg
    12961 ttcgagtcgc tggcgcacat gtgcagcgcg gtgctgtccc gctctgccga acgcgctcgc
    13021 ccacgcgagc cggcgcattc ggatgcactg accgggttgt tcagccgcca tgcgatcgat
    13081 gccgttgccc ggccgatcct gcgcaacgac ccgctcgatc cacgggcctc ggcgctcctg
    13141 atcgacatcg accagttcag tcgcatcaat gacgaatacg gcccggcctg cggcgatacc
    13201 ctgctgtgca gcctcgccga ccggttgcgg cgcggcttgc gcaagggaga cgatgtgttc
    13261 cgctgggagg gtgcgcagtt cctggtgtgt ctgccacgca ccgacctggc ctcggccacg
    13321 ctggtcgcct acaagttgct ggacgccatc gcgcagcctg cccacacgct ggccgacggc
    13381 cgcagcgtgc cgctcacggt gtcgatcggg gtggccgaac tcggcgtcga tgaggcgatc
    13441 gccggtgctg tcgtgcgtgc cgacgcagcg ctgcgggaag ccaagcgaca agggtgcagt
    13501 cgggtctgca aggcccccgc actggtggtg ggtggcggcc caccccaggt cgatgcccgc
    13561 aacgcgccgg ggcgccgggt gattaccggc atcactcgaa ctcgggcatc gcttccctga
    13621 tcgcctccgt cgtgttggac gcatgcgcgg cgatctgctc atggttgcgc gcgatgacgt
    13681 gcagcgggta ggtcccggac acgatgccga ggtaccagac ctcggcgccc accacctccg
    13741 gtgccagccc ctcgaccgcc gccaccagtg cgtcggtcag cgacgcgagt tcggcatcgg
    13801 caaacagcgc ttcgccttca taggtgaccc ggtagtcgaa cgagtggccc tgcggcgagt
    13861 cgatctcgat gcgcacgatc atgaactgct cctggtgtgt ggccttgacc cagagcgtat
    13921 gttggcatgg ggttcacgcc cgcaccgatg acccctccgt cgacggagtc agtccagcct
    13981 gcaccagacc gaagtgcctg cggcgcaggt gacctcaaag cgggtcgccc ccgcgggctg
    14041 ccagtgcatc ggttcggtgt ggccttcgag ttcaggcagg gtggatcgct cgatgaacca
    14101 ccggccatcg cgcagcaggc cgtaagagat ctcgaagtcg agcagtgccg gggccgatgc
    14161 cggatcccgt gacagcagtg tgctcagcgt gttgccgggg tccgttcccg ccggccagcc
    14221 gatccgccgc gcccgcacgc gcatcaggca ctcgccgctc agcaggagaa aggcgcgtgc
    14281 cgtcgagagc acaccggcct cgtcgtctgg cgacaaactt gtttcggcca gcaccaacgc
    14341 gggcccgatg gagccaggca gacgctccca gacttcgcgg taggcgccgt gcacgccggt
    14401 ttcgatgacc cggtcgtcgc tgccgaattc catccatccg gcatcgggcg tcagccccgg
    14461 cggctgcatg tcgagtcggc gatgccaggt gcacacatcg ccatggaggt gctgcacgac
    14521 ctccgtgcgg ccgaagaatc cttgctgatg cgccagcgcg agatgctgct ccccgctcat
    14581 cgcgtcgagt gcgatggccg ccctgtcaac ttcggctgcg gcaggcaccc gcaggtcggc
    14641 atgccagcgc ggagtctgca gccagtggac ccaggtcgtg gtgtctcgga tgtccggtgt
    14701 ttcgagcagc gtgcggcgcc agacgccccg atagcgttcg ggcacctcgg gaaaagtgtc
    14761 gatggatgtc acggggagcc tttcgccatc ggtgctgtcg cgtccggcgg gatcaatcga
    14821 tgaactgcgg aaacgccggc agcgagcgat agaacgcgaa atcgtggttc ggccacagcc
    14881 aggcgccggt ttcggcagcc agcgccttga gcttgcggat gctgtcgatc gcctccgcct
    14941 cgcggccctg ccagagcgtg ccgggggcga tctcgtcgtc gatgttctcc tgcaggtcgg
    15001 cggcgtctcc ggccagcagg atcggcggac ccttcggcat ctcgacccac atggacatgt
    15061 gaccgatcgt gtggccgggg gtcgcgacgg cctgcactcc aggcaccagc gtgtactcgc
    15121 cggtctgcag ccggaactga agaggcgcac ccgcttcgtc gatcaggtcg tcggcgaaca
    15181 ccgccgggtc ggcgccgctg atgcccacct ccatctcgtc gcgctgcacg tgaatctcgg
    15241 cgccgcagcc gcagcggcgc agatcacgca gcccgccggc atggtcgaaa tgcaggtgcc
    15301 cgatgaagat gacgtcgatg tcggccggcg tcaggccgag ccgcgccaga tgtcgaggaa
    15361 tgcgctgttc gtcgcgcatg tccggagcac cgaactcgaa ggtcgccggg ttgtagtggt
    15421 acgcccgtga accgggatcc gcgatcttct cgtggtcgca gcccacgtcg tacaggatgc
    15481 gaccgttcgc ggtctcgatg aggtaggcga ggatcggcgc ctcgatctgc tggccgcgcc
    15541 cgcgctgcca ggtcgagatc gatttgtcat agcgatgcgt tgctgtcagc agcggccaga
    15601 gtttgcgcac ctgggtcatg gaagctcctg gtccttgcag ttgagcaacc gagcgaccag
    15661 cgtcgcgctg tcggtcaccg tgccgaagat acctccctcg acggcgatca tggcgagcga
    15721 aggcgcttgc agcgcagctt ccgatgcggc gcaggcgtcg ccgacggtga tgcagtgcag
    15781 gccatggtcg gtcgcggcgc gcagcgtggt gtgcacgcag acctctgtgg tcacgccgca
    15841 gacgatcacg gtctcggtgc cccagccgcg caggatctgg tcgagatccg tctggtggaa
    15901 ggcgctgtag ccgggcttgt cgaccaccgg ctcatcggcg cgaggctgaa gctcgaggac
    15961 cagtccgtga cctggctcgc cgcgcaccag caggcggccg agcgggccgg ccgagccgat
    16021 ctcggcgccg gcgttccgac tgcgcgcgag cttggccgcc ggacagtccg acaggtcggg
    16081 ccggtgccct tcgcgggtgt agacgatgtg cagtccggcc tcgcgggccg cgtcgatcag
    16141 cgcgcacacc gggccgagcg tggcgcgcag ccgcgacacg tcgacgcccg cacgcgccgc
    16201 atagccgagc ggatcgagga agtcgcgctg caggtcgatc accagcaggg cggtgcgcgc
    16261 tgcatcgagc atcgttcgac cgcctcagcc gaactcgtgc gtgatggccg ccgcgatccg
    16321 ggtcgaaagc tcctgcgcca tcaccgcgcc tttctcgacg ctcgacccgc gcgccgacga
    16381 cagcacgccc gacagcggca cccagtgcct gcgggtcggg tagacgtcgt acggcgggaa
    16441 gtcggctggt ggttcatcgg gtatcaggtc caacctcacc aggttcgggt ggtagtgcag
    16501 catcatcgat gtctcgatga cggccgcatg ttccagggcg aagccgggga agccttcggg
    16561 gaacacgctg gcgagcgtgt cctcggacag gaagtcccag tactcgagcc gcatgacctg
    16621 caacgccgcc cgagggccca ggtcgcgcag cccgagatcg atgccttcgg tcaggaacca
    16681 ctggttctcg tagtggccgt tgacgatcac cagtttcgtc gccccgtgcc gggcgaactc
    16741 gcgcactgca tcgcgcaccg agccgatcag ggtggccgca tcgacgctgg tggtgccgca
    16801 gaagtgctgg ccgccgccgc acttgggctg cgacttgtag ccgtacgaca gggtcggggc
    16861 aaccagccct ccgatgcggg cagctgcatc ggccgcgacc gcagcggaca gcagtccgtc
    16921 ggtaccgagc ggcaggtgcg ggccatgctg ctcgagcgca ccgaccggca gcaggatcgc
    16981 gggctggtgc agcttcagcc gagcctcgta gtcgacccat gaaagctggt tcatccagac
    17041 ggtactcatc ccgctttctc cttcgatgtc gattcgcttg aatcggtggc ttcgatcaat
    17101 gcggcgacgg cgatcagtgc gccgccggcc cattcgatgg gacgcaacga ctcctcgccc
    17161 aaccagacgg cggagaccac cgcgaccagc aactcgacgg tcaggatcag gcccgcgcgg
    17221 ccgctttcga ggtgcgtcac accgtattgc caggtgacgg tcgcgagcag caaccagatg
    17281 aaggcgtagc cggccagggc cagtgcgatc agcgcggtga tcggcggcat ctctgcgcca
    17341 gccatcagca cgctgccccc ggacaagagg ccgcacccga agaacaccgc caccgtcttc
    17401 gatgtcatcg ggatggcctg ggcggcgcgc gagatcaggt tgttgccggc gaaggccaga
    17461 cccgcactca gcgccagcca gtcggcggtg cccaggggct ttgcccaggc cgcgctgccg
    17521 ccgatcacga accacaggcc gaccagcgcc agcaccaccg ccagtgcgcg gcggcggctg
    17581 atcgcctcgc ccagaaacag tcgcccgccc aggaccgacc agacgggtgc caggtagaac
    17641 agcagcatca cccgcaccac gtcgcccatc accagcgcct ggacgaacgc ggtgttggcc
    17701 cagccgccca ccagcatcag cagcagaaga agggcggtct gcggcttcca tgcagggcgc
    17761 tcgcgccaca gcagtccgat gcccagcaag ccgaccgccc catagctcag cagcgagagc
    17821 atcggtcccg acagcccgac gcctgcaaag cccttcagtg gccaccacag caggccccac
    17881 agcacggcag aggcgagcag aaccagcgtg ggcagacggg tgccgggcga gggcggggcc
    17941 aaccgggaga tcagcggcga tgaggtgacg gtcatcgggg acgtcctgcg ctcaggccat
    18001 caccgagccg ccgttcgggc tgagcacctg gccgcaatag aagcgcgaca ggtcggacag
    18061 caggaacagc gccgtgcaag cgatctcgtc ggccgcggcg aaacggtgct gcgggatgtc
    18121 gagttccttc tcgatccact cggggctcat gtgctcgggc gacagcatcg cggtggccac
    18181 cggcccgggt gcgatcgcat tgacgcggat gtccggtgcg aattcccgtg cgagcgagcg
    18241 cgtcagcccg atcacgccgg ccttggccgc gcaatagggc gcgaattgcg cgcggcccag
    18301 gatgccgagg tcggaggcga tgttgacgat caccccggcc tggcgcgcca gcatccccgg
    18361 cagcaccgcc ttgcacatca ggaacaccga cttcaggtcg gtgtcgagca tgcggtccca
    18421 atcggcttcg gtggtctcga gaaaggggcg gtcctggatc acgccggcgt tgttgacgag
    18481 cgcgtcgatc ggccccagtt cggaagcgac cgccttgacc atggcggcca cgtcgcgtgc
    18541 aaggctgaca tccgcctcca cggcaaacgc gcgtgcgccg ccggcttcca gttcggcgac
    18601 cagggcgccc gcttcttcag cgcggccgag gtggttgatg gccacacgcg cgccggcccg
    18661 cccacaggcc agcgcggtgg cccggccgat accgctcgct gcgccggtga ccagcacgac
    18721 gcgcccggtc agggcaagct ggaatgcctg tggcggcagg ctcgctgccg cgttcaattc
    18781 atgacctcgc catggctggc gctgatcgcc tgcccggtca gcgatcgcgc cgcatcgctc
    18841 gccaggaaca ggaacacccc tgcgatgtcc tcgggctcga gcatccgggg aatcgcctgc
    18901 cgcgcgagga tctcgcgttc gacgtccgat tcgctgcgtc cctgggcggt ggccatgctg
    18961 gtcagcgatc gcagggccgc agcggtgcgt atccagcccg ggcacaccgc attgacccgg
    19021 atgccgaggg gcgccagttc ccaggccagc gatcgagtca ggccgatcac tgcatgcttg
    19081 ctggccacgt aggccgagaa atcagccacg ccggtcagcc cccagatcga cgactggttg
    19141 atcacgcagg ccccgcgagg catcaggggc agcagcgtgc gcgtcaggcg caccatgctg
    19201 ccgacgttgt tctccagcag cgccgaccag cgctcggttg cctgggggtg ggtgtcgtcg
    19261 aggggtgtcg ggtattcggt gccggcgttg ttgacgagca catcgaccgt gccatggctg
    19321 gcctgtatcc gtgaggccaa agcctgcagg gcgctgtccg ggccgaggtc ggccgcgatg
    19381 gcggtgacgt gatgcgcctc gagcgagtcg gccacgcgct gcagggcgcc ggcgtcccgg
    19441 tcggtgatga tcagtcgcac ctgctgcgcg gcaaaggcgg cggcgagggc ctgtccgatg
    19501 ccgcctgctg cgcccgacag gaccacggtg aggccctgca gtccgaggca gggcgcggtc
    19561 cgtccgcccg cgcgcgccga gggatcgatc gacacggtca gaccgcggtg aggaacgaga
    19621 cgccgaccgc accgatgaag ccgtcggcgc gcccgggcac ctcggggcca cggacttcgc
    19681 cgtaatcact cgacagcacc cgcgtgactc gatgctggtg aaaggccttc agcagctggg
    19741 gcaccgggat gacctggccg tacgcgttgc cgtagatctc gcggtggtag gcgggaaggc
    19801 ggtgccaggg cacggtcggg cgttcgtggt gtgcgttgtg gtagccgaaa ttcagccaca
    19861 gcaggttgag ccagaagttg tcgagaccga cgacattgct gaacgtgttg gcctgttcgt
    19921 aggcgcggtc gcggaccttg tcgtcgggaa tcggggcatc gccgaggatc gggtacgcgt
    19981 cgtaggtgtg ctggaagcag tcagcgaacc gcagcaccgt gacgaacagc agataggcga
    20041 cgaagtacag cgccagcgcc ttgggcgagt acatgcccag cgcggcgaac gcgccgaggc
    20101 gcaccaccgc gatcccgatg atgcgggcgc gtgccgcgtt ggagccgccg gccctgaaag
    20161 gcatcgcgac gacgaaaccc cgcatcagga attcgaccgc cgggaagtag gcccactcga
    20221 gcgccagcac gccgcgtcgc agccaggccg gcgcggccag caggaaaccg cgcgcgtcga
    20281 acgtgatcac gtcggcgcgc tcgacatggt ggcgcatgtg cttgcgccga aggtcggcaa
    20341 acggcgcgta gcagctgccg ttgagccagg tcatcagcgt gccccaccgt tcgttggtct
    20401 gcggacttcg gaagatggca tggtgagcga actcgtggat gtagtacgcc gaccagacca
    20461 gcgtgagcgt caccagcgca aagccgagcg cattcagcac ccaggacgcc gctgtcagca
    20521 tcgcgatgcc agccgggaag cccagaaggg tcagggccag cgccgcgatg ttcggcgcgg
    20581 cgccgtccgg ggtgcggaaa ctcacttctt caccatctcg gtgacgaact gcgcatcgaa
    20641 ggtcgcttcg gtggcaggca ctgtggtgat ctggcccttc gccttcagca gctgcgagat
    20701 gatttcaccg ctggcgtagt aggaggtggt ctcaggtgcc ttggtgaagg ccttcggcat
    20761 ctcggcgagc gggatgttgt agacgccgga ccactgttcc ttgacttcct tcgccgagac
    20821 gcccatcgcc ttgccgatga tcttcgctgc gtcatccggc ttggccttga tgtaggccag
    20881 gccgtcgagg taacccttga tcacgcctgc gatctcggtc ggcttggcct tgatgacctt
    20941 gtcgtcgaac accaggacgt ccgcgatcag gccgggagca tccttcgacg agaacacgac
    21001 cttgaacttg ttgccaccgc cctggctgac gatctgcgac aggctcggct cgtaggtgac
    21061 gccgatcgag agctggcccg acgccatcgc tgccggcacg gcttcaggcg tcatgttgac
    21121 cgctgtgatg tccttgtcgg acaggccatt gaccttgagc gcataggaca gcaggaagtc
    21181 ggaaggcgac agcgggttga agccgacctt cttgcccttg aaatcggcca ccttggcaat
    21241 gctcttgtcg gccacgatcg catcgccgcc gttcgagtag tcgatcggca tcacgacctt
    21301 ctgcggccgg cctgcggcca cctgaccgac cacctggtcg taggtcagca tgccaccgtc
    21361 gaccgcgccg ctggcgatgg ccgacgggat cagcgccggg tcggtgaaga tctgcaggct
    21421 gaccttcagg ttgtgcttct tgaagaggtc gagcttttct gccacgtaga acggcccgta
    21481 gccgatccac acgacggtgc cgatcttcat cggcttgacg tctgctgcgt ggctggcaac
    21541 cgcgaaggcg gccagagcca gacccgctgc cggtttggcg agcgacgagc ccaggcggcg
    21601 ccacagcgcc gaggacagag cagaagagcg taggggacgg atcaattcca tgtcgagggc
    21661 tccaggtgaa agaaacgggc gcaggagaag acgtttcgat gcgtcgatct cccgggcttt
    21721 tatccctccg tggaacctcg atggctgttg accgccgaag ccggatcact ctcggaccag
    21781 caccggcatc gctgccgatc ggaaccctag tgaccctgag tgagaggagg gcatgcgcgt
    21841 gacatacgaa ctcgctccac ctgcacacga ggtaatgcaa tccatatgcc agataagtgg
    21901 cggctgcgac gcaccaagat gtggcatccg cggtcaggaa gacgcggaca ggtgcgcccg
    21961 ccagccgccc agcgccgtga tgtccttcgc gtcgcgcacg gcgtggcttt cgcagacgaa
    22021 gccctggacg gaagtgccgt cctcgagcag caccgtgccg atgccgagcg gggccgggat
    22081 gccggcgacg aaggagccga actcgctcgc cggcagttcc cacacctcga gctcgatggc
    22141 gccgcccccg gtgtcgttcg cgtccaccct gaccatgcca gggcggaaag gcggcccgcc
    22201 cggcaaggcg tacaggcggt agacgggagc gctgcgcacg gcacgcacca gcatggcacc
    22261 tcgctgcgtc agctgccagt tcaggggcag cccgctcaga tgcgcgccgc acacggcgac
    22321 ctgaacctgg cccgaaggaa aaccgcgcgc atcgggggca cccgcagaaa ggtgcggcgc
    22381 gtggggtgtc gcgccgacgg tggcgaccgt gcgcgcgtgc agtcgcgacg ccagcgtcag
    22441 cagcggcagg tctgccccgc agcgcccgat cagcgtgacg ccgtgcggca ggcctcgggc
    22501 cgggccctgg tcggcgaagc cgaccggcac cgcgaccgcc gacaggtcga gcagattgac
    22561 gaagttggtg tagtggccga gctgcgagtt cagcgcgatc ggatcggcct cgacggcggc
    22621 gatggtgtgg atcgtcggtg ccgtgggcac gaccaggcag tcgaggtcgc cccacgccgc
    22681 cagggaggtt tgctgcagct ccttcagtcg gtacagcgcc gagaacgtgt ctgcagcggt
    22741 cagccgggca cctgcttcgg tgatcgcacg agtcaccggg tgcagggcct cgggttgtgc
    22801 ttcgatgaag gtgcggatcg ccgcgtagcg ctcggccacc cacgggccct cgtacagcag
    22861 cctggcgacg tcgcggaagg gagtcagatc gatctcgatc ggcatgccgc ccagcgcgcg
    22921 caggtgctgg atcgcctgtg cgaattgagc agtcccgctg tcgttgccgt agaagaccag
    22981 gtcctgcggc cgtggcacac cgaagcggaa cgacgtcgcg ccgaagttgc ggccgtgcgg
    23041 cggcatcgtt cgcgaatacg cgtcctgcgc gtcgggtccc tgcgccgagg ccagcaccgt
    23101 cgccgcatcg gcggccgtca gcgcgaacac cgagaccgca tcgagcgtgc gacaggccgg
    23161 gacgacgccg gtggtcggga tgcgccccag gctgggcttc agcccgatca ggttgttgaa
    23221 cgcagccggc acgcgacccg aacctgccgt gtcggtgccg agggaaaagc tcgcgagacc
    23281 cagtgccacc gaaacggcag acccagacga cgagccgccg gacacgaagg ccggatcgat
    23341 cgagtttctg caggccccgt agggcgaacg cacgccgacc agaccggtcg cgaactggtc
    23401 gaggttggtc ttgcccaccg ggatcgcacc cgcgtcgatc agccgctgta ccaccgtcgc
    23461 gctgcgttgc ggcgtgtagg cgaaggccgg acaggcggcg gtggtcggca caccggcgag
    23521 gtcgatgttg tccttgatgg cgaacggaat gccgtacagc ggcagcgtgg ccgggtcacg
    23581 gccttcgagc gcgctggcat atcgcatcag cgcgtcgaga ccgaggcgat ggatccagac
    23641 gttgcgcggc agggccgcct cctcggcatc gatccgggcg tgcagctcgg tgacgagccg
    23701 ggtgggcgtc agctcgccac tcaggtagcg cgtgcgcagc gcaggcaggg aaaggtcggg
    23761 tcgcatcgga cggtccttcg ataacgatgt cagcgcagca ggaccagcgg ttgcccggcg
    23821 gtgaccggct gtccctccga gcaaagcact tcgtgcacca cgccggcgcg cggcgcgagc
    23881 acggtgatct ccatcttcat cgactccacg acgagcaagg tgtcgccggc ctgcaccgca
    23941 ctgcccttgg cggcgagcac cgaccagacg ccacccgaca cttcggaggt gatcacttcg
    24001 ccatcgaacg cactggccgc ggcggcgcgt gcgtcgagat cggcatcgac ggtctcttcc
    24061 accgtctcgg cgacgccggc ggcccgccag cgttcgcgtt cggcttcgaa ggcggcctgc
    24121 tggcgggtct tgaactcgct gatcgctgcg ccgttctcca ccaggtatcg ctggtagtcg
    24181 gccagcctga acgtgccgtc ctcgatgcgc aggccggcgc ggcccgcgac gaaatccgag
    24241 cgcagtttca gcaactcgtc ggcgctgacc ggatagaacc gcaactggtc gaagaaccgc
    24301 agcagccagg gcttgccggg ctcgaactcc gaggtgctct tccagcggtt ccacatctgc
    24361 accgtgcggc cgacgaactg gtacccgccc gggccttcca ttccgtagac gcacaggtag
    24421 gcacctccga taccgaccgc gttctccggg gtccaggtgc gggccgggtt gtacttggtg
    24481 gtgacgagcc ggtgccgcgg atcgaggggg gtggccaccg gcgcgccgag gtagacgtcg
    24541 cccaggccga gcaccatgta gctcgcctcg aacaccgtgt cgtacacggc ctggatatcg
    24601 tccagcccgt tgatgcgtcg aatgaactcg atgttgctcg gacaccaggg cgcgtccgca
    24661 cgcaccgact gcatgtactt ctcgatcgcc agccgcgtcg ccgcgtcgtc ccaggacagc
    24721 ggcagccaca cggtgcggct cggcacctcg atgtcggcgg tcgccggcac gccgtcctgt
    24781 gcgtcgagca gcgcgtccat cagggtggcc agcgcgatgc gcgacgggtc gtagtgcacc
    24841 tgcagcgagc ggatgcccgg tgtcaggtcg atgaccccgg ccagcctgcc ctcgtcgcgc
    24901 cacgactcga gcaactgcat cagcgcgtgc acgtgaaagc gcagggtcag gtcgagcttg
    24961 agctcgccga actcgatcag cacgtaggca tcgcccgcgc ggcgatagac gatctcgatg
    25021 ccgtcgtccc tggcggcccg gtgatgcagc accgcggcat cggacagcaa cgcgcgagaa
    25081 atgggcgcag cggcgggcgc ggtgttcgca aggggggcgg ttgccgacag caccacgtcc
    25141 tgcgcggcgg ccagcgcacg cgcctcggcc agggtcagcg gcttgaagcg cacggtgttg
    25201 cccggcgaca gctggccgag cttccacagc tcggcctgca ccacggtggc cgggcagacg
    25261 aacccaccga ggctcgggcc gtccgggccg aggatcaccg gcatgtcgcc ggtgaagtcg
    25321 atcgcgccga tcgcgtacgc gttgtcgtgg atgttcgacg ggtgcaaccc ggcctcgccg
    25381 ccgtcgctgc gcgcccaggt cggcttcggg ccgatcagcc tgacgccggt gcggctcgag
    25441 ttgtagtgaa cctgccagtc cgtcgcgaag aaggtggcga tgtcgtcggg cgtgaagaag
    25501 tcgggcgcgg catgcgggcc gatcagcacg ccgatgtccc agtgggtgcc gcaggccggc
    25561 gtcagcatcg ccgcctgttc ggcgctgatc ggcgccgggg ttgccgatac tggttgtccc
    25621 agatgcagca cgtcgccgcc gcgcagcacg cgcccgccgt gcccgccgaa ctggccgagc
    25681 gtgaaggtcg atccgctgcc cagatattcg ggcacgtcga tgccgcccgc caccgccagg
    25741 taggcacgac agcctgccgc accgctctgg ccgacgcgac cgatgcgcag gttctgaccg
    25801 gcctcgaccg tcaccgcgcg acccatcggg atggcctttc catccaggga caactcgatc
    25861 ggcgcgccgc agaccgcgac gacggccggg cacctgaagc gcagggaggg gccggcgacg
    25921 gtgatctcca ggccggcggc gccttgctca ttgccgagca gccggttcgc cagccgcaac
    25981 gccagcggat ccatcggccc ggacggtggc acgccgacat cccagtagcc gagtcgacca
    26041 ggccagtcct gcaccgaggt ctgcacgccg gggctgagca cgtcgaaggc gcgactcggg
    26101 gcgctgaagc tggccaggct gcgggtcgtg tgcatgccgt ccgcgaacgg cgcaaacgac
    26161 atcacggcgc gcaggtactc gaggttcgat tccagcccgt ccacatgcgt gacggccagc
    26221 cccttcgaca gcttcgcgat cgcgtctgca cggtcgctgc cgtgcgcgat cagcttggcc
    26281 agcatcgggt cgtagtaggc ggtgacttcg gtgccgcgtt cgatccaggt gtcgacccgc
    26341 aggtcagcgg gaaactccac ccgagtcaac acgccggtgg ccggctggaa gttcttgaag
    26401 gggtcttcgg catacaggcg cgcctgcacc gcatggccgg tttgagtgac gcgcaggctg
    26461 tcgagcgcag gcatctcgcc tgccccgagc cggaccatcc attcgacgat gtcgatgccg
    26521 gtgaccgcct cggtcacgcc gtgctcgacc tgcagccgcg cattcacctc gaggaagtag
    26581 aagtcctgcg tgctcgcatc gacgacgaac tcgaccgtgc cagccgatcg gtaatgggct
    26641 gcctgagcca gtcggcgggc cgtggcgtgc agtgcggcgc gcacctccgg cctcaggtgc
    26701 ggcgccgggg cttcctcgac gaccttctgg ttgcgtcgct gcagcgaaca gtcccgatcg
    26761 cccagcgcaa gcacctgccc cgcgccgtcg ccgaacactt gcacctcgat gtgtcgcgcg
    26821 tcggtgatgt agcgctcgat gaagacgccg gcatccgaga aattgctctg cgcaaggcgc
    26881 ctcacggtat cgaaggccga gcgcagctca tctgcgcctg cgcacatctg catgccgatg
    26941 ccgccgcccc cggcgctgct cttgagcatc accgggtagc cgatctgctc ggcgcgggcc
    27001 agcgcctcgc tcagatcgga cagcaggccg gttccgggca gcagcggaac cccactgtct
    27061 gtggccagcg cgcgcgcggt gtgcttcagg ccgaacaggc gcatctgctg cggcgcgggc
    27121 ccgaggaaga ccaggcctgc ggcctcgcag gcctcgacga acccggcgtt ctcggacagg
    27181 aaaccgtagc cagggtggat cgccttcgct ccggtggcca gggctgcctc gatgatgcgt
    27241 gcaccgtcga ggtaactcga cgccaccggc gccgggccga tgcacacgga ctcgtcggcc
    27301 atggacacgt gcagtgcgcc cgcatcggct tcggaataga ccgcgaccga gcccacgccg
    27361 aggcggcgca gcgtgcggat gatgcggcag gcaatggcgc cgcggttggc tatcaatacc
    27421 ttgtcgaaca tggtggctca gacggaatgc aggtcgtcct gcgttctgcg aatgcacgcg
    27481 ccggtcgtcc ggcgatgctt ggctttcagg ccgggtccca caccaggaag cgcacgggag
    27541 tcgggttgta gccgttgcat gggttgttca gttgcgggca gttcgacacg agcacgacca
    27601 catccatctc ggcgcgcatc tccacgtact tgcccggcgc ggagatgccg tcctcgaagg
    27661 tcagtttccc ctcgggcgtg accggcacgt tcatgaagaa gttgatgttc gagaccacgt
    27721 cgcgcttggt cagtcgtggg atgcctgcgg tggattcgct gcccgaatcg atccgggcca
    27781 gcgcgagcat gaagctgtcg cggcagctgt gcatgtagcg cttctcgagc gcatagcgaa
    27841 ccgtgttgct ctcgcaggcg caggctccgc ccagcgtgtc gtggcggccg caggtgtcgg
    27901 ccgtgacggt gagcatgggc ctcgcctcgc tcgacatcag caccgagccc acggtcaggt
    27961 acagcgcccc ctgggcctgg atcgtgtggg tcaggctgta tcgctcgccg acgtcggcgg
    28021 cgctgtagaa gatcgtgtcg acggcctggt tcccctcgat atcgacgatg cgcaaggtct
    28081 ggcccttctt caccaccttc gcccaggggt cgccggcgag gcagatctcg tcgatcaccg
    28141 catcggcagg gagcagcggg ctttccacgg tggcggccat cacaggaacc acctttcggt
    28201 gttgatgtag ccccggccgt tctcggggca atggagacgg caggcatcgt cggcgcccgc
    28261 ggtgcctgag cgccaggcgg tcaggtgcac gggtgtcggc gcataggatg tcgcgggatc
    28321 aagcgggtga ggcgcggccg acaggatgac cagcacgttc atctcgaagc gcacgtcgac
    28381 gtgctgtccg ggcgcgcggt gcgcggtgtc gaagtgcaga cggccggtct cgtcggcgac
    28441 ggccttgctg aagaaattga tgttggcgac caggtcgcgc cggtccattc cccatttgcc
    28501 cagttcgacc agcaagccgt cctgaccgtt gcggtgcatg tcgttgcggc gttgctggta
    28561 gctggcctct ccgtagcgct gacgcacggt gtccgcatcg atcacgccgc agacggtgtc
    28621 gtgccagccg caactgtcgt cgggaatcga gcacagcacc cggcccatgt ccgaatacag
    28681 cgcatgtccg cgcgtgagat gcgcggtgtg ctgcgccttg agcgtatcgg gcatgttgta
    28741 gcgctcggtc ttttcttcct ggttgaagaa cagcgccgag acgttggcgc ggccctcgac
    28801 atcgatcagt cgcagcgtgt tgccgcgccg cagcaggccc gaccagtgcg cgccgccggg
    28861 cagggtctcg tcccacagca cgaggtcctg cgcgtagggc gtcgatcgat ccggcacggc
    28921 cgcccgggcc agtggggaca gcgaattgaa atgggtttgc atgggtcgtc aggcgtcagg
    28981 ttccggatgt cacagcgatt gcccctgctg ggaaatacgc tccagcaggt cgaggtcggt
    29041 ggccatgccc gagctttcgc ggatgtgctt caggatctcg gccttcagtt cgcggaacgg
    29101 tgcgccatgc ttcatgtcct gctggcggcg cgccggcagc ggcaccgggt agatgctgtg
    29161 gatgcgaccc gggcgcggct ccatcagcac gacgcgctgg ccgaggtaga tggcttcgtc
    29221 cacgtcgtgg gtgacgaaga cgatggtggt cttctcgatg cgatggatgt gcaggatcag
    29281 gtcgtgcatc acttcgcggg tctgcgcgtc aagcgcgccg aagggctcgt ccatcagcag
    29341 cacctgcggg cgtcccatca gcgcacgggc gatcgccacg cgctgctgca tgccgcccga
    29401 caactggctc gggtacgact tcgcgacctt ggccagcccg atcaggttca gcagcgcttc
    29461 ggcgcgtccc gatgcggcct cgacatccga agaggtcagg ttctcgcgat tgacctgcag
    29521 cgttcggctg aacttgatgt tctccatgac cgtgagccac gggtacaggc tgtagtgctg
    29581 gaacaccatg gcccgctcgg ccccgggccc gctgacaggg cgcccgttgc agatcacctc
    29641 gcccgaggtg tggtgttcga ggccggccag gatccgcagc aaggtggatt tgccgcagcc
    29701 ggaggctccc accagcgtga tgaattcgtt gtcctcgatc gacaggtcga tgtcctgcag
    29761 cgcggccggc gtgccggctg cagcaccgct gaacaccttg ccgaggcggc ggatctccgc
    29821 cttcggggag gggacttggg gggcaatgga ctgcatgggg cgactccggc tcagcgcttg
    29881 aagtgcatgt acgggaacgc ccggcggtgc agccagcgga aagcctggtc catcagcagt
    29941 ccgatgacac cgatcaggat gatgccgccg aagatcgtgt cggtcttcag gaaacgctgc
    30001 gccttcaata tcgcgtagcc cagccccgaa ttgctggcca ccagctcggc caccaccagg
    30061 taggtccacg cccaacccat ggtgatgcgc aacgtgtcca ggatggctgg cttggccgac
    30121 tgcagcagca ccagccggac gatctcgtcg cgcgtggcac ccatcgtctg tgccgcctcg
    30181 atctgcgccg tgggcaccag ccggacgttc tcggccacca tcagcaccat ctggaagaaa
    30241 gtgccgatga agatgatcgc gatcttggcg ctttcctcga tgccgaacca gagcatcacc
    30301 agcggaatga aggccaccgc cggcatgtag cggatgaagt cggtcagcgg ctcgagcagg
    30361 gcctcgacag ggcggaaggc gccgatcaga agcccgatcg gcagcgcgaa cgccgccgac
    30421 agcgcaaagc cggccagcac ccggtagacg ctgataccgg tgtcggcgat caggtcgtct
    30481 tccatcagcc aggtcccgag ccgcgcgaac acggaacccg gccccggcat gaagaccggg
    30541 tcgatccagc ccgatgcaga gagcagcgcc cagactgcga acggcaggat cagcccgaga
    30601 gctgcaaacg cccagtactg tgttcgggaa agccgccccg caatcgccca gatgctggga
    30661 gtcgggggtg gcttcgccgc acctgcgtga ggggtgaagt tcgagcccat gggaggtgct
    30721 gtggcagaca tcgatcgctc caggacagtg gcacaacgcg agagggcagc tgcgtctgct
    30781 gacgacaact tcctcccggg cttttgtccc tccgtggaac cgcgatgtat tgcggccgac
    30841 cgctctcgga ccagtccacc cggtgggcta ccaggcacgg aaccctagct gtcttgctga
    30901 tcaagcagat ccgccgaggc gactgccact ctcgcaacca aactaagcaa tgtcggtgcc
    30961 agccggtgac gtgtgggcag ggatgccgcg attccatgct gccaccggcc agagcgtccg
    31021 ggtcggcgct gcagcttgtt cggcccgaat tccagcgacg gaagccactg gaagtcgctg
    31081 gactcgaacg cgccatgcgc cccagcgccg gacgggtgcg caccgaaatg gggcgcattt
    31141 tcgtgtgctg ctgtggaacc tgcggcaggc gccttgagtc accagtccta accgctgcgg
    31201 cgtcacttcg cctccctctt cgcggggaaa gacgaaaggc ggcgattgca gggacaatca
    31261 gcaccccgtc gacgacgccc gagtcgaccc cgttcaacga gacttgtcag cttgccatgg
    31321 ccacacgcaa ctccaaaaca agcccacccg tcgacggcgc acagaaactg cgacagacac
    31381 aggccgagta ttccgcctcc cgtcccgcgg cggagcccgg cgcccgaacc atgatgtcga
    31441 gctcgaagat gtacgtgtgc cggcgttgtc acgccaattt caagaccggt gatggcaagc
    31501 cggacctgcg catgcccggc ctgggcaagt gcaacgcctg cgtcgctgcc agtgcggctg
    31561 ctgcagccgc ggcgtcgggg tctgcgccca cttgatcgaa agcccggcat cgcctgagcg
    31621 cgccggcgat cccggcgacc tggcgctggt gttcggggcg cggggcggtc ttggtgcggc
    31681 acttcgagat cgcctgcgag gcagcgcgcg atatgcccag gtgctggcgg gcggtcgtgg
    31741 cggcaccgtc gacatggcga tcgacatcac cgatgaatcc agcatcgaat cggcggcaac
    31801 gcaggtggcc gagcgactcc agcagggctc gatactgcgt ctggtgatcg atgcgaccgg
    31861 cttcctgcac ggccggggct tcgagcccga gaagtccctg cggcagatcg atgcggcgca
    31921 catggcccac gcgttcgcca tcaacgcgat cgggccggca ctgctgatga agcatttcct
    31981 gcctctgctg ccgcgctcgg gccggtccgt gtttgccacg ctgtcggcga aggtcggcag
    32041 catcggagac aaccacctcg gaggctggta cagctaccgc gcgtcgaagg ccgcgctcaa
    32101 tcagctggtg cgtactgccg ccatcgagct ttcacgtcga cagccgcagg cgatctgcgt
    32161 ggcgctgcat ccgggcaccg tggccactcc gctgtcgtcg ccgttcgcga agtcggggct
    32221 gcaggtccag gcgcccgatc tggcagctca gcgtctgctg gcggtgatcg atgccctgac
    32281 cgccgaggac tctggcggat tcttcaacca ccagggtgaa cgtctgccct ggtgatcgag
    32341 ccgtcaagca gccttcactg ctccacgaag gcccgttcga tgacgtagtc cccgggcttc
    32401 gaggtgttgc cttccttgaa gtgccgcgct tccatcatcg ccttcagatc gcgcagcatc
    32461 tcggggcttc cgcagatcat cacccggtcg tgctcgggat cgagcgcggg caggccgagg
    32521 tcggcggcca gcttcccgtt gtcgagcagt gtcgtgacgc ggccctggtg gacgaagggc
    32581 tcgcgggtca ccgtcgggta gtagagcagc tggttggtca ccatctcgcc caggaactcg
    32641 tgtgccggca gctcctcggt caggtactgg tgataggcca gttccttcac ttcgcgcacg
    32701 ccgtggatca ggatgacctt ctcgaagcgc tcgtaggtgt cggggtcgcg gatgatgctg
    32761 aggaacggag ccacgccggt gccggtgccg atcaggtaca accgcttgcc cgggagcagg
    32821 tagtcgatca gcagcgtgcc ggtgggtttc ttgcccacga tgatcgagtc gccgacctgg
    32881 atgtgctgca gccgcgaagt cagcgggccg tccggcacct tgatgctcag gaactcgagg
    32941 tgctcgtcgt ggttcgggct gacgatgctg taggcacgca gcagcggctt gccatcgacg
    33001 cgcaggccga tcatcgtgaa atggccattg ctgaaccgca ggctcgggtc gcgggtggtg
    33061 gtgaagctga acaggcggtc ggtccagtga tggacgctca gaacgcgctc ttcgttgaat
    33121 gcactcatgg gtgggccggc aagtcgacaa aagggtctat tttccacgcc gccggcccgc
    33181 tgcctgcgca gatgcaaatg agtttttcgg gggcatgatc cgtcaggccg gagacgaccc
    33241 ctgccgtctg atgccgaacg gaattccgcc atgaccgacc acgccgccgc ccgtttccag
    33301 tgggaagacc ccttgctgct cgattcgcag cttgcagccg acgaacgcca ggtgcgcgag
    33361 gccgctgccg ccttctgccg tgaccgactg gcgccgcggg tgctcgaggc cttccgccac
    33421 gagaccaccg atgtcgcgat cttccgggaa atgggtgagc tcgggctgct gggcatcgtg
    33481 atcccgcccg aatacggagg cgcgggcatg aaccacgtct gttacgggct ggtggctcgt
    33541 gaagtcgagc gtgtcgactc gggctaccgg tcgatgatga gcgtgcagag ttcattggtg
    33601 atgctgccca tccatgtctt tggcagcgag gcgcagaagc agcagtacct gccgaagctg
    33661 gcggcagggc aggcgatcgg cagcttcggc ctgaccgagc cggaccacgg ttccgacccg
    33721 ggcggcatgc tgacacgtgc ccgcccggtg gccggagggt ggcgtctgag cggcaccaag
    33781 acctggatca ccaacagtcc gatcgccgac gtgttcgtgg tctgggcgaa ggacgatgcg
    33841 ggcgcgatcc gcgggttcat cctcgagaag ggctggcccg ggctgagcgc ccctgcattg
    33901 cacggcaagg tggggctgcg caccagtgtc accggccaga tcgtgatgga cgaggtcttc
    33961 tgccccgcgg agaacgcgct gcccgacgtg cgcggactca aggggccttt cacctgcctc
    34021 aacagcgcgc gctacggcat cgcctggggg gccctcggtg cagccgagga ctgctggctg
    34081 cgggcgcgcc agtacgtgct ggaccggcag cagttcggcc gtccactggc cgcgaatcag
    34141 ctgatccaga agaagctcgc cgacatgcag acggagatcg cactcggcct gcaggcctgt
    34201 ctgcgcctgg gtcgcatgaa ggacgacggc agcgccgcgc cggagatcac gtcgatgctc
    34261 aagcgcaatt catgcggcaa ggcactggac atcgcccgca tggcgcgcga catgctgggc
    34321 ggcaacggca tcagcgacga atacggcatc gcgcgccacc tcgtcaacct ggaggtggtc
    34381 aacacctacg agggaacgca cgacatccat gcattgatcc tcggccgcgc gatgaccggg
    34441 attgccgcct tcgcgaactg atccggctct tcggcatgcg cggagggcgc ctgtccagcg
    34501 cagccttcac gcggcattaa ggcgcaggtc ctaagctggg tcgctcgcgc cggctcttct
    34561 gcaggcgcac ggccgttccg agaagacccc acccatgcca ctgagccagc cgctgtccgc
    34621 cgaggcgacc gaaccccggc gccgcggtcc ggcacgtctg acgtggtcat tgcccaccga
    34681 agccgtggtg ctgatggcaa gcctgttctt cgcgctggtc agcaaccagc cgttctggtc
    34741 cgccgccatg gcgggtcgcc gcctcgacga ccccggctcc tggctcttcg gggcctgcgc
    34801 cttcctgttg ttgctggtga tccacttcgt ggttctgagt ctggtgtcga ctcgtcgaac
    34861 cgtgcgcccg ttgctgacgg tgctgctgct gggtaccgcg gcagcgtcgt atttcatggg
    34921 tcggtacgcg gtcttcctcg acccgacgat gctgcgcaac ctgctgcata ccgacaccgc
    34981 cgaggcgcgt gatctgctgg cctgggacat gctgcctcac ctgacgctgc aggccgcgct
    35041 gccgatctgg ctgctgtggc gcatccgtat ctgcgaccga ccctggcggt cggccgcgct
    35101 gtggcgagca ggtgcggtcg ccggtggtct gctgatcggt gtggctgcct tgctgacggt
    35161 ctctcaggac ctgtcgtccc tgatgcgcga acagaaggcc cttcgctatc tggcgacgcc
    35221 tgcgaactat gtctattcgc tcacccgggt cgccacggcc gatgcggtcg gcgcaacgcg
    35281 accgctgacg cagatcgggc tcgatgcagt ccgagggcca gcctggtcgc gttacaagaa
    35341 gccgctgctg ttcgtcgtgg tggtcggcga aaccgctcgc gccgccaact gggggctcaa
    35401 cggctacgca cgccagacca cccccgagtt ggcgcaactc ggcgtcttca acttcaccga
    35461 cgtcacctcg tgcggcacca acaccgagac ctcgttgccc tgcatgttct cgcacatcgg
    35521 ccggcgcgac tacgacgagg cccgcattcg tggcgaggag tcgctgccgc acgtgctcag
    35581 gcacgccggc ttcgaagtcg agtggcgcga caaccaatcg ggttgcaagg gcatctgcgc
    35641 ggggctgcca tcccagaaca tggccaagtc gaccgatgcg aaactgtgcg acggtcacga
    35701 gtgcctcgac gagatcctgc tgcagggtct ggatgcccag atggccaacg accagggcag
    35761 ccgggtggtc gtgttgcacc agatgggcag ccacgggccg gcgtatcacc ggcgatcacc
    35821 ggcggccttc cagcaattca agccgacctg tgacaccagc gaactgcgca aatgcaccaa
    35881 gcagcaggtg gtcaacagct acgacaactc cgtcctctac accgaccacg tgcttgcgaa
    35941 gacgatcgga ttcctgaaat cccagagcga tcgctatgac accgcgatgc tctacgtgtc
    36001 cgatcacggc gagtcgctgg gcgagaacaa cctcttcctg cacggcatgc cctacgcgat
    36061 cgctccgaag gagcagacgc acgtgccgat ggtgatgtgg ttctccgaca gctacgccag
    36121 cagccgtggc atcgatctgg gctgcctgcg cggccgcgcg gtgcagcatg cgtcgcacga
    36181 cgacctgttc cacaccgtgc ttggcatgct cgatgtgcag acaaagacct atgaaccggc
    36241 gctcgatgtc ttgcgaacct gccgtgtcgc agccgcgccc tgaggcttga ccttcggcat
    36301 cgccagctgt gccgagatgc gctcctgccc gtgatcgcgc aggaactcct tgaacgcgag
    36361 tgcgacttgc ggcagctgct gtgaactgag gtgcatcacg caccagtcgc gctcgatggg
    36421 attgcccggc atcggcaaga gctgcagcac accgccctga cgttcgagca cgcaggtgtg
    36481 catcgacagg aaggccacgc cgaatccggc ggcacacatc tgcttgatcg cttcattgct
    36541 cgacatctcc gagccgatgc gcaactgcag gtcggcgtcc ttgaagatgc gttcgaccgt
    36601 ggtgcgggtt ccggagccgc gctctcgcac caggatgcgc tccttcccca ggtcggagag
    36661 gctcagcccc tggtgtgaca tcagcggatg gttcggtgcc gcgaggaacc ccatcgggtg
    36721 cttggcgaac accagcgagg tggtcttcaa ctcggcgggg gggcgaccca tgatcgccag
    36781 gtcgatctcg tggccggcca gcatccggac gatctcgtcg cggttgccga cctgaaggtt
    36841 gaacttgatc ttcgggttgt cgttggcgaa ggcgacgagc agcggtggca gcaggtattc
    36901 ggcggtggtc accgcgccga cgcgcagcgt tcccgagaag acgcctcgca gcgtcgccat
    36961 ttcctcgcct gcctcgcgcc acagctccag gatgcggccc gcgtacttgg ccagcaactc
    37021 gcctgcagcc gtcggtcgga ttccccggcc ggtacgcatc agcaatggcg ttcccgcgga
    37081 ctcttcgagg atgccgatct ggatcgacac cgcaggctgc gtcaggtgca gttcgtcggc
    37141 tgcgagcgac acgctgttga gccgtgcgac ggtgtggaag gtggcgagct gacggaaggt
    37201 ggcgttcttc gagagcggca tggcggcact ccagggcaag gaagcatatt agccagacac
    37261 aatgcatcag tcaaaaagtt tcacttttgc aaattcgaga gctgcgtcat tcttcgcccc
    37321 gttccaagtt gatttaccgc cgcaacaccc gaggagagtc ccgtgggcca catgaacgaa
    37381 ccgcagcaga tcaccgacag caagaagcgt tattcggccg gcgtgctgaa gtacgcgcag
    37441 atgggttact gggacggcga ctacgtgccc aaggaaaccg acatcctggc gctgttccga
    37501 atcaccccgc aggacggtgt cgatccgatc gaggcagccg ctgccgtggc tggcgagtca
    37561 tccaccgcaa cctggaccgt ggtgtggacc gaccgcctga ccgcctgcga catgtaccgc
    37621 gccaaggcct accgagtcga tccggtgccc aacacgccgg ggcagtactt ctgctacgtg
    37681 gcctacgacc tgagcctgtt cgaggaacgc tcgatcgcca acgtgaccgc gtcgatcatc
    37741 ggcaacgtgt tctcgttcaa gccgctgaag gcagcacgcc tggaagacat gaagttcccg
    37801 gtcgcctatg tcaagacgtt tccggggccg ccaaccggta tcgtcgtcga acgcgagcgt
    37861 ctggataagt tcgggcggcc gctgctgggc gctaccacca agccgaagct gggcctgtcg
    37921 ggccgcaact acggccgcgt cgtctacgaa ggtctgaagg gcggcctcga cttcatgaag
    37981 gacgacgaga acatcaactc gcagccgttc atgcactggc gtgatcgctt cctgttcgtg
    38041 atggacgcgg tcaacaaggc cagcgccgcg accggtgaag tcaagggcag ctacctgaac
    38101 gtgaccgccg gcacgatgga ggagatgtac cgccgcgccg agttcgcgaa ggagctcggg
    38161 tcggtgatcg tgatgatcga cctggtgatc ggctacaccg cgatccagag catgagcgac
    38221 tggtgtcgca acaacgacat gatcctgcac ctgcaccgtg caggtcacgg cacctacacc
    38281 cgccagaaga accacggcgt gtcgttccgt gtgatcgcca agtggatgcg catggcgggt
    38341 gtggaccaca tccatgccgg cacggccgtc ggcaagctcg aaggcgatcc gatgacggtg
    38401 cagggctact acaacatctg ccgcgacacc tacaccaagg tcgacctgcc acgcggcatc
    38461 tacttcgacc aggactgggg cgcgctgcgc aaggtgatgc cggtggcatc gggcggcatc
    38521 cacgccggcc agatgcacca gctgatcgac ctgttcggcg acgacgtcgt gctgcagttc
    38581 ggcggcggca cgatcggtca cccgcaaggc atccaggccg gtgcgaccgc gaaccgcgtg
    38641 gcgctggaag cgatggtgct ggcccgcaac gagggtcgcg acatcaagaa cgaaggcctc
    38701 gacatccttc gtgacgcggc caagtggtgc agccccctga aggccgcgct cgacacctgg
    38761 ggcgagatca gcttcaacta ccagtcgacc gacacgtccg actacgtcgc gacgccgtcg
    38821 gtgtcgtgat cgatcagcgc aaaggaaaca ccatgcgact cacacaaggc accttctcct
    38881 tcctgcccga tctcaccgac gcgcagataa gcaagcagat cgactattgc ctgtccaagg
    38941 gctgggcgat cggtctcgag tacaccgacg acccgcatcc gcgcaacacc tactgggaga
    39001 tgttcggaaa cccgatgttc gacatctcgg atccagcggc gatcctgatg gaggtcaacg
    39061 cctgccgtcg gacgtttccc gaccactaca tccggctgac cgcgttcgat tcatcgcatg
    39121 gcaccgaatc cgtggtgatg tcgttcatcg tcaaccgccc gaagcacgaa cctggcttcc
    39181 gcctggtgcg ttccgagcag gccggccgca cggtgcgtta cagcatcgag agctacgcca
    39241 cgcagtcgcg tccggaaggc atgcgctact cgtcggacaa ctgaccaaga ccagcccgca
    39301 ccatggacgc ttccacgccg ccatcgtcga cgccggagac cccccgggcg ctgctcgaat
    39361 cctccggcgt caagacggtg ctcgaccagc tcgacgcgga gctggtcggt ctggtgcctg
    39421 tcaagagccg catccgcgac atcgccgcgc tgctggtgat cgacaagctg cgtcaggaca
    39481 tggggctgca ggcgcaacct ccatcgctgc acatgtgctt caccggcaac cccggcaccg
    39541 gaaagacgac ggtggcgatg cgcatggccg aagtgctcaa gcagctgggg tatgtccgca
    39601 aggggcatct ggtggcggtg actcgcgacg acctggtcgg ccagttcatc ggccacaccg
    39661 cgccgaagac caaggaagtg atcaagaagg cgatgggcgg cgtgctgttc atcgacgagg
    39721 cgtactacct ctaccgaccg gacaacgagc gcgactatgg gcaggagtcg atcgagatcc
    39781 tgctgcaggt gatggagaac caccgcgacg atctcgtggt gatcctcgcg ggctacaagg
    39841 accggatgga gaccttcttc ggcagcaacc cgggcatggc ctcgcgcatc gcgcaccaca
    39901 tcgacttccc cgactacagc gaagccgagc tgatgcagat cgcccggctg atgctcgaga
    39961 agctcaatta tcgattcgac gccgacgccg aacccgtctt cgggcgctac atcgggctgc
    40021 gccgtcagca gccgcatttc gccaatgccc gctcgatccg caatgcgctc gaccgcatcc
    40081 ggctgcgcca cgcgacacgg ctgtttgcgc acgacacctc gctgacgcgg gatcagttgt
    40141 gcacgctgca ggccgaagac atcctggcca gccgcgtctt cgcggcggcc gcaccacccg
    40201 ttactgcagg aaattgacat gagcatcgaa gccctgatct tcgacgtgga tggcacgctg
    40261 gccgataccg aggaggcgca ccgcacggcc ttcaacctgg cgttcgagcg ctttcgactg
    40321 ggctgggtct ggggccgcga cgagtaccgc gagctgctca agatcaccgg cggcaaggaa
    40381 cggatcaccc actaccttcg ctcgctcacc acggtcggcc cggccgaatt caagcgcctg
    40441 ctcgacctgg tgccggacct gcatgccgaa aagacccgct tctacagcaa ggtggtgggc
    40501 gatggcggtg tgccgctgcg cgagggagta gctcgcctgc tcgatgaagc gctcaacgat
    40561 ggctgtcgcc tggccattgc gagcacgacc accgccatga acgtggatgc actgttgcag
    40621 gccaacctgg gcagtcgagg gctggagaag ttcagcgtga tcgcctgcgg cgatcaggtg
    40681 cgctcgaaga agcccgcgcc ggacatctat cttctggccc tgaaacacct gggcctgcat
    40741 gccgaacagg cggtggcctt cgaggattcc tacaacgggt tgcgctcggc tgtatcggcc
    40801 gggttgcgca cgatcgtgac cccgacgttc tggaccgagg gcagcgactt cggtgctgca
    40861 acgctggttc tgccgacgct gggctcgccc gcccatccgc tgatcggaga gccgggcggg
    40921 cggctgcgca acgaaggctg gctgagcttc gcggaagtgg tgcaattgac ggcccggccc
    40981 aagctgccca gcgcagtgag ggcgctctat caaggaacca cttcatgaat tccgcctcgc
    41041 cgcacccgga aatgagaatt gcgcccagcc tgctgtccgc cgactttgcc cgcctgggcg
    41101 atgaagtcac ggcggtcata gaggccggcg ccgacgtgat ccatttcgac gtgatggaca
    41161 accattacgt gcccaacctg accgtgggcc cgctggtctg ccaggccatc aagccctatg
    41221 ccgtcaggag cgggcagccg gtgccgatcg atgtgcacct gatggtcaag ccggtcgatg
    41281 cactgatccc gatgttcgct caggcgggcg cctcgatcat ctcgttccac cccgaagcca
    41341 gcgagcacat cgatcgcacc atccagctga tcaagtcgct gggcgtcaag gcgggcctgg
    41401 tgctgaaccc ggccacgccg ctgagctgcc tcgaccatgt cctggagcaa ctcgacctcg
    41461 tgctgctgat gtcggtgaac cccggattcg gtggacagag tttcatcgag catgtgctgc
    41521 cgaagatcga ggcggtgcgc aagcgcatcg acgcgaccgg acgcgacatc tggctcgagg
    41581 tcgatggcgg tgtgaaggcc gacaacgcgc ggcgtgtcgg tgccgcaggg gcagacacgc
    41641 tggtggccgg ttccgcggtg ttcagcggtg gccgctacaa ggaatcgatc gaagcgatac
    41701 gcaccaatgc gctcgccgga cggcactcgc ggcaggcgaa ggacccggtc tgacgatgaa
    41761 gcccgacctg agctgcatcg ctgcgattgc cttcgatctc gatggcacgc tggtcgacag
    41821 cgcgcccgac atcggtcacg ccctgaacac ggcgctggcg gatggcggtc tgagctgctt
    41881 cgatctggcg acggtgcgaa cctggatcgg tgacggcccg gacgccctga tcgcccgcgc
    41941 gctggccgcg caggacgtgt cgcccgatga cgccgggctg cgccagaggc tgcgccgcgg
    42001 cttcgacgag gccacgctgg cggcgccgct cgacaacggc tgcgtctatc ccggcatcgc
    42061 agacctgctg gacagcctgc acggtgtgct gccgatggcg gtggtcacca acaagccgac
    42121 gcggctggcg caggcggtcc tcgaggcagc cgggttgagt caccacctga tgcacgtgca
    42181 gggcgccgac acgccggccc agcgcaagcc ggcaccgagc atgttgctgt ccaccgccgc
    42241 cagattcggc ctgcagcctt ccaggatgct gatggtgggc gatgcaccgc ccgacatgct
    42301 ggctgcgcag gcggccgggt gtcttgcggc gctggtcggc tggggttacg gcgcgcatgc
    42361 ggtgccttcg gcctcgcgcc cgtggcgggt cgagacgcca cagaaactcg gcagcagcct
    42421 cctcgccatg gcgcacccgt cgcgcccatc accgacggtt tcgatcgact gaggagcacg
    42481 ccatgcccac cggaggaaag tccaccctga cccagttcct gatcgaggaa cggcgccgtg
    42541 cccccgaggc ctcgggcgac ctgaatgcct tgatcaccgc cgtctcgctg gcctgcaagg
    42601 ccatctcgcg cagggtggcc tatggcgcgc tggcgaccgg ctccgatgct gcaaccgcgg
    42661 cctcgcaggc tggcaacctg aatgtgcagg gcgaggaaca gaagcctctg gacatcctga
    42721 gcaaccagat gttcctgcgt gccaacgagt ggggcggaca cgtcgccggc atggtctccg
    42781 aggaaatgga ggagatgtac acattgccgg cgggataccc gcgaggcaag tacctgctgc
    42841 tgttcgatcc gctcgacggc tcctcgaata tcgatgtcaa tgtcgcggtc ggcagcatct
    42901 tctcggttct gcgtgcggtg acgcccgagc aggaccccaa gacgggcgac ttcctgcagc
    42961 ccggcacgcg gcaggtgtgt gcgggctatg cgatctacgg tccatcgacg atgctggtgc
    43021 tgagcatggg ccggggaacg cacgccttca cgctggaccc gctgctcggc gaatgggtgc
    43081 tgagccatcc gaacctgcag atcccgaaga agacacgcga gttcgcgatc aacgcgtcga
    43141 acagccgctt ctgggaaccg gccgtcaagc gctatgtcga cgagtgcctg gccggcaaga
    43201 tcggtccgcg cggcgccgac ttcaacatgc gctggattgc gtcgctggtg gccgagacac
    43261 atcgcatcct gatgcgaggc ggcgtgttca tgtatccgcg cgacaccaag gacccttcga
    43321 agtcagggcg cctgcggctg ctctacgaag ccaacccgat ctcgatgctg atcgagcagg
    43381 cgggcggttc cgccagcacg gggcgcaccc ggcttctcga cgtgcagccc gagtcgctgc
    43441 accagcgcat cggattcgtg ttcggttcgt cggacgaggt ctcgcgcatc gagacctacc
    43501 accgcgaaga accaccggaa acctacagct ccccgctctt cggcaagcgc ggcctgttcg
    43561 ccgcagcaga ctgaaactgt ccagatactg aaacccacga ggctctccca tgtccgccaa
    43621 gcatccagtc atcgcgatca ccggttcgtc cggtgcgggc accacgtcgg tgacccgcac
    43681 cttcgagaac atcttccgcc gcgaggacgt ccaggcggcg atcgtcgagg gtgacagctt
    43741 tcaccgctac gaccgcaagt ccatgaaggc cgcgatggcc gaagccgagg cggcgggcaa
    43801 catgcacttc agtcatttcg gcgcggcagc caacctgttc gaggaactgg aggcgctgtt
    43861 tcgcgactac agcgcaaccg gtaccgggca aagccgcaag taccttcaca acgccgagga
    43921 agccgcaccg ttcggtcagg aacccggcac cttcacgcca tggaatccgc tgccgaccag
    43981 cgaactgctg ttctatgaag ggctgcacgg cggggtatcg acagacacgg tggatgtcgc
    44041 gcgccatgtc gacttgctga tcggcgtggt gcccgtggtc aatctcgagt ggatccagaa
    44101 gatccaccgc gatcgcagca cccgcggtta ctcgaccgag gccgtgaccg atgtgatcct
    44161 gcgccgcatg cacgaatacg tgcacttcat ctgcccgcag ttcacacgca cccacatcaa
    44221 cttccagcgg gtgccggtgg tcgatacatc cgatcccttc atctcccgca cgatcccgac
    44281 ggccgacgag agcttggtcg tgatccgatt cgccaacccg cgcggcttcg acttccccta
    44341 cctgctcagc atgctgcacg actccttcat gtcgcgcgcc aacacgctgg tggtgcccgg
    44401 cgggaagatg gaactcgcca tgcagctcat cttcacgcca atgatcatgc gcctggtcga
    44461 gcgcaggcgg cagttgctcg ctcgctgatg ttccgccagc ggtaggcgat cggccccacc
    44521 gatcgatcgg cattgcaagc cagcggtgca accggccaaa atggggccat gagtaccgtt
    44581 cgagcctgcc cgacctgtac ccagatcaat ccaaccgggt ccgtcgtctg catggcctgt
    44641 ggagcggagt tgtcggctgc ggcacgccga aggccgaaag cgactgaact ctccatcgat
    44701 ctgggtcgcc tcgagtctga agaggacacg gttccagcgg tgcatctgga tgcgcccgaa
    44761 ttgcagcgct gggtcgattc gggccccgat gcgctcgaaa cgacctgcgc ggtgcctgag
    44821 ctgacgcttc ggtccgtcga tgcgctgccg gtgctcgaga acgtggcgga tgcagctgaa
    44881 acgctgccac gcggcgtggt ggctcgctcg gacggcgaac cggccgccgg caaggcgtcc
    44941 gttgcgaagg cggcgcgccg tgcggcggtg cgccgcaacc gcttgtcagg tcatcgccag
    45001 gccggcgcgg ctgccacgcc gaccgatgtg ctggtgctcg gacgcgacga agcggcacgc
    45061 gaagcactct gcggcttgct tgaaaccttc ggctttcggg ctcacccggc ccagactccg
    45121 gtgcaggcgg tccgcctgct cgatgcaaag cattttgtgg cggcgttcct cgatctggtg
    45181 ttcgatggtc cgcaacagcg ggcgagcgtc gacctgtgcc agcgcatcaa ggccgaagtt
    45241 caccaggcga tgggacgggc ctccgcgctg atcatcgttt cgagctcgtc gcgccctgtc
    45301 gagcgcgtgc gagccacgct ggccggctgc gatgcctttc tcgtgcaacc ggtgacccga
    45361 ggcggagtcg cgcaggctct cgaggcctgc agcgtcgcgc tgcctgcgga ctcgcgggga
    45421 aactgatcag cggggttgtt gcctcgaact ggggcgatgg gctttcctga ggacggcgct
    45481 gcgggctttg cggcgcatgt tccagaccga cacggcagcc gcagccagcg cggtggtgct
    45541 cagcacggag ccgaacgacg actcgagcgc gaagaggcac gccgcggcgg tgagcgcgta
    45601 caagagtggc aggctttcgt acagccagtg aggaaaccac atgtcgatcc cttcagtcgt
    45661 tgaactcgcg gtcccgcggc gatccatttc gcccgggcat caagaaaccg ttccgcgatc
    45721 atggggaatc gtaacgagtt gttcaacccc gggactgcgg gtgtgccggg tggcgcaaag
    45781 gcggtgactt gtgtatgcga cagcggcgtg tcaggtctgg atcaggcccc agcgcagtgc
    45841 gatcaacgtg agctccgact ggttcgtcgc attgagcttc tgcttcacgt tgtagagatg
    45901 cgtgccgacc gtgctgggtg agaggctcag gctgtcggcg atggtggcga cgctgcggcc
    45961 ctgggcgagc tggatgaaca cggagaactc gcgctcgctg agcatgtcgg ccgggctggt
    46021 ctcgccgtcg atctgcgcca tcgcgagcgc ctgcgctgtc tgcgcatcga cgtagcggtc
    46081 gccgcgagcc acggccgtga cggcggctat cagggcttcc ggcgcgctgc gcttggcaag
    46141 atagcccagg gcgccggctc gcagtacccg gcgcgggtgg gccgtgtcct cgtgcgccga
    46201 cagtgcgagg atgcgcgcgt tcggatcgtg cgcgagcagc cgacgaaccg cctccaggcc
    46261 ccccatgcct ggcatggaca ggtccatcac gaccacgtcc ggcagcacct tcgggtactc
    46321 caggcaagcc tgttcaccac tgcccacttc ggacacgacc tcgacctgcg catctccgag
    46381 cagcatcttg aagcccatgc gcaccagcgc atggtcgtcc accagcatca cacggatcat
    46441 cgctctgtcc tcatgaatgt tcggcctcgg caacttgcgc ggcaggcaat ggcagctgca
    46501 cctgcacgag cacgcctcgt gacggggccg gaccgacctc gaccacgccg cccagcgcct
    46561 ccacgcgctc ggacagccag cgaagcccat ggtgaccgac tcgctgcgcc cagtcggctg
    46621 gcaagcccga gccgttgtcg gccaaccgca gcagcaccgt gtccgcctgc cgccgcaccg
    46681 tcagggccag ggcggtcgca tggccatggc gcaatgcatt ggtgatgccc tcctgcgccg
    46741 accgatacag cgccagcgcg cgctcggtgt cgaggggggt gtccgccaga tcgacctgca
    46801 actcgatctg tacctgcggc tggctgcgcc gcgtgcgctc gaccagatcg gccagcgctt
    46861 cggccagtcc gaagttgtcg agcaccatcg gcgtcagccg cgggatcatc ccgtgcatcg
    46921 cgtcgtacag gcgcgccgac tcctcggcga tcagctgcgc agcctgctcc gactgagggt
    46981 ccgcacgcgt gcgtgcgcgc tgtgcgatcg acagcgccat gctgcgtatg gctgtcaccg
    47041 attggcccag ctcgtcgtgc agctcgcgtg cgatcagcag gcgttccttc tcgatgtggt
    47101 gatcgaccca gcgcgtcagc tcccggttgt ccgacaactg ccgttcggcg cgcgccgcgc
    47161 gtcgctcggt ctcgatgttg tcccgcagca caccgaccat gcggttgaag gcggcgccga
    47221 tcgcagccgc ctcggtgccc ggcaatgcag gcaacgcaac atcgaagcga ccgccctgca
    47281 agtcgttcag cgcacggacg atctgcgaga acggccggac cgtgctgccg accagccaga
    47341 acaccaggac gttcgccgcc gccagcaggg ccattccgat gagcgccaga tgcacgaagt
    47401 agtcccaagc atcgagcact gcgcgcgagg catcgctgcg cacgaccagc ttgcctccgg
    47461 ggaaatcgat ggcctggacc gagggcggcg gtgccaccag tccgacaaac cagtcgggcg
    47521 cctcgcggcc agacttgtac ggcgacggcg gcgaggcgta ctgccggcgg ccgccttggt
    47581 cgaagagctc gatgtcgttc gcacgcaccc gccccatgcc ttgcagaaag gcacgcatgg
    47641 ccggcagacc ctgtgcggca tacagccaga gcgtgcggtt gagcaattgc gacgcgacgc
    47701 ggttggctgc cacgacctcc tcggcgaccg actccttcag gctgcgaacc tgcagcccga
    47761 gcaccgcggc gacgaacatc agcatcagca caccgacgat caggttgatc ttgacacgca
    47821 ggttcacgaa ttcacctcgg gttcgaagcg gccgcttgcg gcgtaggaat cgagtgcccg
    47881 cagcatcgcg gcgaccatct ccgggtgcgt cggatcgtgg ccgacgcccg ggatgacgcg
    47941 taacgtgctg tgcggcaggc gccggtgcaa ctcgcgtgca ccatcgggcg ggcagatgtg
    48001 atccgaaccg ccatgcagga tcgcggtcgg cacgcagggc agggcggcgc agcgatcgag
    48061 cagcggcggc gtgtccagcc agcagcagtg gctcaggtag tggctctgca cgcggtaacg
    48121 gccgatcagc gcctgcagcg catcgccttc gggctcgtcc ggtggcagcg gcgcgcaaga
    48181 catcgctgcc tcccagcgcc gccacgccag cgcgaacggc gccgcctgtg ccacgtcgcc
    48241 ttgcagcccc tcggtcagca cctcgagcaa cgcgtgccgc cgcgccggcg gggcgatcgc
    48301 tgcgaagcgt gtccaggctt cgggccggtc ggcggctgcg ccctggaaaa agtgggcgat
    48361 gtcctgggcg cgcgccagaa acgtggcgcg cagcagcaaa gccgacaccg cgtgcgattc
    48421 ggccagcgca tgcgccagcg agagcgtcgc accccacgag cccccgacca ccagccagcg
    48481 ttcgatgtcc aggtggtggc ggatggcttg caggtcagcc agcaggtcgg ccgtcgtgtt
    48541 gtcgcgtgtc gcgccgcgcg gcgtgctgcg cccggcgccc cgctgatcga agccgatgat
    48601 gcggtagcgc gcagggtcga gaaaacgcac cagcagcggc gacatccccg agcctggtcc
    48661 gccatgcaac accaccgccg ggatgccgcg caagttgccg cactcctcca catgcagcgt
    48721 gtgtacgtcc gacactcgca gcgcatgggt tcgcagcggt ggtgtcgcgg ggtacagcac
    48781 ggcggcagcc cgggaaaaca ctgggagtga ctcggcggaa ttcaccgagt cgatggtagc
    48841 ggcggcgcct caccgtcatc gcccggggat tcatgaaaat catgaggcgc aactcatggc
    48901 cgggtcgcta gtcaggcggc ccgaccccgg ccatgatggc ctccgggatg agacgttccc
    48961 gcagccacca accggcgcct gcgccacctt gcaaggagac ttcaaatgac ctggaccaca
    49021 ccttcggccg ccgacttccg tttcggcttc gaaatcacga tgtacgtcgc cgcacgttaa
    49081 tccgtcgtcg cgtctggggt ctctcaacag acctcaacca acgccctggc tgatcccagg
    49141 gcgttggtct ttcagggtca acccttctcg atctccgaag tcatcatgcg catcacagtc
    49201 ttgggttcgg ccgccggtgg cggatttccg cagtggaact gcaactgcgc caactgcgcc
    49261 ggaatccgcg ccggcacctt gcggggcaag ccgcgcacgc agtcatccat cttcgtgcgg
    49321 cctgacgaca gtgccgacgg cgtgctcttc aacgcgtcgc ccgacatcct cgaacagatc
    49381 cgcaacagcc cggtgatgca gcctgcgcgc catcttcgcg actcggccat cgcaggcgtg
    49441 gtgctgatgg acggccagat cgaccacgcc acgggcctct tcatgctgcg cgagcgcaat
    49501 tcgaaactgc cgctgtggtg taccgacccg gtcgccgaag acctgacgca gggcaatccg
    49561 gtgctgcgcg tgctgacgca tttctgcggg gtcgaccgcc acgcgatcac gctcgacggc
    49621 tcgtcgttcg aggttcccgg tgtgacggga ctgagcttcc gggcgctgcc cctgtcgagc
    49681 aaggctgcgc cgtactcgcc acatcgcgac aacccggtgc ccggcgacaa catcggcatc
    49741 gtcatcaccg accagcgcag cggcaagacc gcgttctacg cccccggtct cggcgagatc
    49801 accacaccgg tgttcgccga aatgtcgcgt gccgacgcgg tgctggtcga cggcaccttc
    49861 tggaccgatg acgagatggt gacgctcggc atcagcaaga agcgatcgcg cgagatcggc
    49921 cacctgccgc agtcgggcga gggcggcatg atcgagtgga tgtcgaagct gccgccgaag
    49981 acgcgccgcc tgctgatcca catcaacaac accaatccga tcctcgatga ggactcgacc
    50041 cagcgcgccg aactgacgcg cgccggcatc gagttgtgcg aggacggcat gctgatccaa
    50101 ccttgatcgg cccgaggagc gcgccatgcg agcagacatg attcaggaat cccgccaccc
    50161 cgacgccgcg cgatcgggtc aagccgacga caacgcggcg tggagccgcg aggcgttcga
    50221 ggccaagctg cgcgagcgcg gcaagtcgta tcacatccac catccgttca acgtgatgct
    50281 gaacaccggc aaggccacgc cggagcaggt gcgtggctgg gtcgccaatc gcttctacta
    50341 ccagatcgcg ataccgatca aggacgctgc ggtgctgtcg aactgccccg atcaggcggt
    50401 gcgtcgcggc tggatccagc gcatcctcga ccacgacgga ttcgagatcg gcggcatcaa
    50461 ggatcccggc ggcatcgaag cctggttgcg ccttgcgcag gccgtgggtc tgacacgcga
    50521 agaggtgcag gacctgcgcc acgtggtacc ggcggtgcgt ttcgcggtcg atgcgtatgt
    50581 caacttcgcg cggcgcgcac cttggcagga gtcggtgtgc tcgtcgctga cggagctgtt
    50641 cgcgcccgag atccacaagc agcggctggc gacctggccc gagcattacc actggatcga
    50701 atccgaaggg ctcagctact tccgcaaccg cgtcggacag gcgcggcgcg acgtcgagga
    50761 ggggctggcg atcacgctga accatttcac gacgcggccg ctgcaggagc gcgcactgga
    50821 aatcctgcag ttcaaattgg acatcctgtg gacgatgaac gacgcgatgg cgacgcgcta
    50881 cggggtctcc tcatgacgac ctccacagtt gcgcaaggca gcctgctgtc gaacacagtg
    50941 cgccccaccg tcggccccgg ctttcgcctg caatgggagc cggtgcagaa ctgccacgtg
    51001 atgctgtacc ccgagggcat ggtcaagctc agccagagcg ccggcgagat catgaagcga
    51061 tgcgacggcc agcgcagcgt cgccgagatc gtcagcgacc tggagcaggc gttctcgaca
    51121 cagggcctcg aaggcgacgt ggtcgccttc atcgagatgg ccggcaagaa ccgctggctg
    51181 gtgtggtcat gagcggaccc gtgatgtccg ccgcgccgcg ccccggcccg ccgctgtggc
    51241 tgctcgccga gctgacctac cgctgcccgc tgcactgcgt gttctgctac aacccggtcg
    51301 atttcgcccg caaggaagac gagctgtcga ccgaggactg gttccgcgtg ctgcagcagg
    51361 gtcgcgagct gggcgcggtg cagtgcggac tgtcaggcgg tgagccgctg gtgcgcgacg
    51421 acctcgagct gatcgttgcc gaagcgcatc gcctgggcta ctacaccaac ctgctgactt
    51481 cgggtgtcgg cctgaccgca gagcgcgcgt cccggctgaa ggcggccggg ctcgaccacg
    51541 tgcagctgtc gttccaggac tcgacgaagc agatgaacga cttcctgtcg cacaccaaga
    51601 ccttcgacct gaagaaccgt gtcggcaaga tcatcaagga ccagggatgg cctatggtga
    51661 tgaacgtcgt catccaccgc atgaacatcg accacatcga tcgcatcatc ggcatggccc
    51721 atgagatggg ggcggagtat ctggaactgg cgaacacgca gtactactca tgggcgatgg
    51781 tcaatcgcga tcacctgctg cccacccatg cccagctcaa gcatgccgag caggtcaccg
    51841 acgcctggcg cgcgaaactc ggcaacgcca tgcggttgtt cttcgtcgcc cccgattacc
    51901 acgagggcaa ggccaagaag tgcgtgaacg gctggggcag catgttcctg accgtggtgc
    51961 ccgacggcac cgccgtaccg tgccacaccg cgcagatgct gccggggctc gatttcccga
    52021 acgtcaagga gcacagcctg agcgagatct ggttcgattc cgagggcttc aatcgctacc
    52081 gcggaaccgg ctggatgaag gagccttgct cgagctgcga attcaaggaa atcgatctcg
    52141 gaggctgccg ctgtcaggcc tacatgctga cgcaggaccc gactgccacc gacccggtct
    52201 gcatcaagag cccgcatcat cacctcgtga ctgatgcggt ggcccaggcc gatgtggtcg
    52261 ccgccaaccc ggtgaccgaa cacccactgg ttttccgcga ccgcgtcaac tcgttgcgcc
    52321 tgattgaaac cgagtcccca cactgctagg ctccccggct ccaccaacac aaagagatgg
    52381 gagactcccg tgggattttt tgacttcctg ttcggcaaga agaaggtcga gcccgcacgt
    52441 gtatcgacgc ctgcgcccgc ccccgcaccc gcgcctgctg cgaagccggc ggtggcaccc
    52501 gcgcccaagc tggcgccccc gatcaccaac gtgcgcgttg gcgaatcgct ggtcggcgaa
    52561 ggcaacgagg tggcccacat cgatctgctg gtcgggccgc gcggctcggc agcggaaaca
    52621 gcgttcgcca atgcgctggt gaacaacaag gacggtttca cctcgctgct ggccgtggtc
    52681 gccccgaacc tggcaaccaa gccgaacgcg gtgatgttca acaaggtcac gatcaagggt
    52741 gccaagcagg ccgttcagat gttcggcccg gcccagcgcg gagtcgccat ggccatcgcc
    52801 gattgcctgg ccgagggcac gctgcgcgcc gaccaggcca acgacctgtt catctcggtc
    52861 ggtgtgttca tccactggat ggccgaggac gacaagaaga tccaggaata caactaccgc
    52921 gcgaccaagg aatcgatcga acgcgcggtc agcggttttc cgacggcagc cgaagtgcag
    52981 gctcagtaca agaccgcgaa gcatccattc ggggccgatt gacccgcgtc cgtgcgctca
    53041 agcagcaaag gcaacggcga cgttgccttt tttcattccc gtgtcactgc acgagattca
    53101 ggatctcgag ttgctctggc acggtctgga atgtgccggt gcccgccggc atctgcatca
    53161 ggggcttgac ctcggtatcg agcagccaga cgctcggata gacgagcaac cgcgcacgct
    53221 cgtcaccgat ggtgcgctgg aattcggcat agggcagggc accgaggttc gttcggatga
    53281 tgagcgcctt ggacgagatc ttcagcgaac tgcgcaggtc gacgatcagg taacccgcga
    53341 agtgcggcaa cagttcaccg gcgttttgcg ccaggaatgc ctcgtaacga cggcaataag
    53401 ggcagtcgtc ggcgccgagg tagatgtaca gcgacttctt ctcgcgccgg gcctggtcga
    53461 tcgccggtcg cagatcgaac tcggcctggg tcggcagtcc ggtcggctgg acaacgatgg
    53521 cgcggtaggg cacaggcagc cccgcgctgc gggccccgcc gtgcgccagc atcagccagc
    53581 acgcgaacag tatggctgcc ccgcaatggc gaagcaggtc agggcgcacg cgaatccaca
    53641 ggcgcgtctc cctgccccgg aagcccggac gcatggacag gcagcatgcc cgtgaagcga
    53701 ccttcgcgag aatcctcgat ctccgccgtc agggtggcat cgccgcgcgg aacgaaattg
    53761 aacctgaatg tcgggttctc gctgaccgag aaatcgaggt cggcatcgaa cagcttgtgc
    53821 tcgccgtagc ggaccttgat cgagcgtatg aagtgcggcg gtatgaacat caccgtgatg
    53881 ttgttcagct cgaacccggt gtcgttggga tgcagcacgg tcacgtcagc cacgttgggc
    53941 cggtcataca cgacaggacc gttgagcttc aggatggtct tgccgatcag ttcgggatgc
    54001 tcacggttgg gtggcgccga gcagccaccg gagaccttca cgtagcgcga gtccatgtgc
    54061 agttcaccat tcgacagttc gctgaccaca cgcacgtggc tgtactcatc gacacgcagc
    54121 cgggtttcga agtcggcctg cccgaggtcg gcggtcaggt ccagcacggc cgccagcggt
    54181 gacgggttct tgtcgatgaa gaggtacagc ttcttcacgt aaagctcgtt gcgctgaggc
    54241 agcttggaca cgaccttgac cggcaccgac gcaccatatg cagcgcgcag cggcacgatg
    54301 agttgaacct ggctggcggc atcggccgcg atcgagcgat tcgggaaaaa cctagcgcga
    54361 agcttttccc attcgggcga cgagtcgttg tgtgccgcgt tcgagttcaa gcctccgacc
    54421 gcggcctgcg ccgggcccgc gatccagccg acggacgcga gcacgcacac catccaaaga
    54481 gcacgccaca gcctcgctga cttcgacatt acaccgctcc gctgatctgc atcacttgaa
    54541 attcacttcg cgttcgacgc gcacgaacag gaccgaagca ttcctccggt ggatcgtgtc
    54601 gtactgatcc cattccttga actcgtccag gctgctggca tcgggcactt cgctgagcgc
    54661 tgccccgtcc atcacgagtt gcctgacacg tgcttcgagc gtgtcgagat accgccgcgt
    54721 ggtggtgatc agcccggatg atgtggcgcg cccgtggccg ggaacgacag tgctaaccct
    54781 cagcgcgtcc atggccgcca gagcattacg ccagcctggc aactggctgt cctgaatgtc
    54841 cgggatgcgc tgttgatcca tcaagccacc ggcgaacagc acgccggttc gttcgtccag
    54901 caccgcgatg tcaccggggc cgctggagtg cccgaagtac atcacccgca ccgggcgacc
    54961 cgccacatca agcgtgtggc tgtcgtcgaa gacctggtcg ggcttgaaca tcgccgtgtc
    55021 gcgcatctcg tcttcgccga gcaggcgccg cagcgtcttc aggcagttcg cgcagcgggc
    55081 ggccatcagg tcggctgcgc tgcgctgcat gcggatcggg atgccccgat cgcggaaagc
    55141 cgctgcgccg aaaagaaact cttggcgaac atgggtgacc agggccagca cgaccggctt
    55201 gtcagtcacc cggcggatcg cctcgagcaa ttcgtcgccg tggcggtgcg acgtgcccac
    55261 gtcgatcacg atgactccgc ggtctccgac gatgaacccg gcgttaccga tgcgcccgag
    55321 gttgctcgaa tcgacttcgc cgccggtgcc gcgcaccatg tacacgccat cggccacctg
    55381 aacggtggtt ccgccggcaa cctggccgtc aggctcggcc gcaaggaacc ggccgcctgt
    55441 ggcgcaaccc gccagcatcg caagcccgaa cgccgcacac gcaaagctga tgcggccgcg
    55501 ccggcgaagc aagctcactg accctcgacg agcttcttca ggttctcgag cccgctcttg
    55561 tagacccccg tcacggcctt cagtgcagct tcgtcgttct gatcgggtgg tggatcgttg
    55621 ttggggaagg cgcggtagaa ggcgccgcgc cactcgacga tggattgcgc cgcgccggcg
    55681 ctggtgacct gcagcgtcga ggtgtagttg gtgacgggca gcgcgccgcc atccttggcg
    55741 cggtagctga acttcatctt cgcctcgtcg aagctttcca gggtttcttc gagcgctccc
    55801 cctcccttca atttcagcgc gcgcaccgag ccttcggtat tgcctgccga ggccggactc
    55861 tcggcaacgg ccgggtgcca atccttcagc gcgtcgaagt tcttgacacg tgcccaaacc
    55921 gctgcggcag gggcattgat ggtgatcttc tcgctgacct tctggcgtgt cggcccatgg
    55981 gcctgcgcca ccgacatcag accgaacaag accacccagg ccatccagcg cactggattg
    56041 gcgttcagct tcgtacgcaa catgctcact tcctcccgtt cacgacatct tgaaattgtt
    56101 cgcaccgggt tcacggtgct ggggcatcgc caatgaaggc gccgaaccca cgactattct
    56161 gaccggtggc aatcgtcttg atcgtctgtc ccgataccgc atcgagcacg ctcaggttgt
    56221 cgtccatcca gttgacgaca tagacacgat cgcgatgaga cgcgatgcct tcgggatagc
    56281 cgaagccgcg caatgtacgc accactttca gcgtgtcggc atcgatcacg ctgacgctgt
    56341 cctcatgctg attggtgacg taaagcagct tcccgccatc cgccattgcg gcgccgtatg
    56401 gggccttgcc gacagccacg gtcgcgattg gcaccagctt gccggtccgg aagagatcac
    56461 gcgtatcgag cacgctgagg tcgttgctct gcacattgag tgcatacagc cgctgtcggg
    56521 gtgtgtcgta cagcaacgcg aagggccggg cacccaccgt cacccgcgac agcacgcgct
    56581 gactgttgac atcgatcacg gcgacgctgt catcgtcgcg ctcggcgacg aacacggcct
    56641 cgccgctctc gtgcaccgcc aagcccgccg gggcacggcc gaccgcgatg ctcatcagcg
    56701 gatggtgcac gcggctgtcg aagaccagca cgcgattgcg gtaccagtcg ctcacataga
    56761 cgcgcatgcc gtcgtcggac gcggcgattc ccagtggccc atcgccagcg gccagcgtgt
    56821 cgaccacctg gcggctgcgc atgtcgatga ctgagatcgt cttcgaatcc gggttggtca
    56881 cgaacacctt cgcctgcgag gcagcgatgg cgacgccagc cggcccgcgc ccgaccggaa
    56941 cggtagcgac cacgctgtcg atcagcaaat cgatcaccga gacatcgtgg ctgccctgat
    57001 tggtgatgta ggcgaacggc gcggcatgcg acgacgcgac ggtcgcgccg aggaggagca
    57061 tgaacgccat cgctcgtgct gcagcccagt gcagcttcgc actcattctt cccattccag
    57121 ttcggcaaag gccgtcgtca cgttgcgctc gtggaagtga tcggccagcg cccagccctg
    57181 caaggcatcc cggccgaccg tcctgaccgc accttgcatc gtggagtggg cacggatcgc
    57241 ggcgcgcgtc tcggtcagca gggcgtcgag ataacgacgc tgaggaccca gcatcgatgg
    57301 ccagtcgcgg ccagccgccc catgacccgg cacagcgagc gccacaggca tctgcgccag
    57361 acgatccatg gcagcaatcc agcccagcag gctgccatcg atgaccggca gatgcgtcac
    57421 gaagagcaga tcccccagga agagcgtgcg tgtctggcgg tcaaaaaccg tcaggtcgtg
    57481 gtcggtgtgt gcggtgggcc aggccttcac ttcaatgaca cgctggccca ggtcgatctc
    57541 gagggtctgg ccgacggcta tcgtcaccgt gggatagacg aggtcatcgt gtttcatcgc
    57601 gaccccgaag tcgcgttgga gcgcgttcag gtaatagcgc tcccgcgcag ccagcgccgc
    57661 acgcatgcgc gcatgcgtca cgaagcccgg gcccgcggcc gaatctgctg tcctgaatgc
    57721 gttgttcccg agcacatggt cgggatgggc atgcgtgttg atgacatggc acaccggcag
    57781 cgggctgatc gcggccacag cggcttgcca ccgacggccg acatcgggcg tgccaccgct
    57841 gtcgatcacc gcgatgcagc gcgtgccgac tatgaacgcg aggttcgcga catcgccggc
    57901 gctggcggaa ttccagtccg ccactgcacc gctgtgcacg aacacgcccg gagagagctc
    57961 ggccacttgc aatggaggca aggcagcttg agcacaggcc agggatgtcg ctgcagccca
    58021 cagcagcaac gcatgccacc aacgaatcac gggcttcgcc tgcgccggta gttgcgacag
    58081 cagaagcccc tgccagggca acccgccggc atcacttcgg gcccgtgccg atgaagcagc
    58141 tgttcttgac cttggcctgc aactcgcgaa accgctttgc ctccttctga acgccctcgt
    58201 tgtcgcgcat cgcgctgcca cgttcgccgc cgatggaggt ggcgttggca tcggtgctca
    58261 gggtgacgta ttcgtcgtac ttgacctgct cggccatgcg gtcgagcgca caggagcatt
    58321 tgctgatcat ctcgaactgg gggccggggt tctcgcgcat gcagtcacgc acgtaaatca
    58381 cgcggtcgag ggtcgggaaa tcattggctt gcagcaccgc agagaaggac gcgcacaaca
    58441 gcagcgtggc gacgcgcgct ggatttctgg aaacagtcat ggaaaagact cacttgatct
    58501 gggtgggaat cacggccgat gcaccctcga cgaagagctc ttcgacgatg acatctggca
    58561 aaccggaggt ttcgccgctg atccgggtgc ggataccaaa gatgccgcct ggcacggcct
    58621 cggacaacaa gaactgatat tgcttgcgga cgaacttctc gtacttggga cgattcggat
    58681 cgtcgaggta gggcgtgaag gtgatttcct ttccggcgat cgtcttgttt ctgtaggaaa
    58741 gcgaagtgtc tcgaaccgtc gcatcctgat aggcagccat gcggatgcgc ttgcggaaat
    58801 ggttcaacga gcccttggtc aaccgcttca tctccccgat gtctctctcc aggaaatgca
    58861 gcagaacggg gtttccttcg gccatcgggg cctcgggcaa cgggagtcga cgggtaccgc
    58921 tgaagaaatc gctcttcacg gtgcagcaag tgccatcggc ctgcttttcg aagctcagca
    58981 cgacggagtc ctcgaagccc tcctcgaagc tgcctgcctt gcggaaggaa taccggatcg
    59041 tcgtcgccgg tttcacaccc gagagctggt ttgacatgaa aagaagcttc tcggctggcg
    59101 agaaatcctg cttgttctca ggcggcttgt cctgcgcgcg ggatgtcgac gcgaaggcca
    59161 tcagcagcgc gaggcacgca ccggcaaatc ggggaaattt catgaactgc atgtgggaag
    59221 gttggaggtg tcttcaaggt caattgggca gaagactctt ggccagctgc agcgcaaggt
    59281 cgggattcag ctgtcgcagg cgccgctgaa cgctttgcgc ctgcgccgtc attccgaggc
    59341 cgctgtagac caggccgagc cggtgccagg ccagagccag cgtgggcgcg atattcacag
    59401 atcgttccaa tgcgtctctt gcatcggtca gccggttcag cttctgcagc gccaggcccc
    59461 gcaggtacca cggctcggac tcgtgctcgt gcatcgcggt ccagcctgag gcgagatgct
    59521 cgagatcgtc ccaccggctg tcgcgctcaa gcacggcggc ctgcaggaag cgcggctgct
    59581 gggtgagtgg ttgttgccag taggccagtt cggtcgcggg gatggaagcg atttcccgcg
    59641 catcagcttg cctggcgagc aggtccttga cccagtccgc gggcgcggag aagtagtgcg
    59701 cctctccacc gcgcagtcga aaggtcagga tgccgaccag tcgcaagtcg tcatcgaaca
    59761 aacctccgcc gctggcgccc gacgtgaacc agttggtgct ctggatgacc cggccgccat
    59821 cgaagggatg cagcacaacc acctcgcccg aactgttctg gatgcccatg ccacccgtgt
    59881 agccgatcgc tgacaccgac tgcccttcgc tgatcgaacc ggatgacccc agaacaacac
    59941 catcgccgct caggccgggc acgatgagca gacagaggtc acgccgcaca tcgctgacct
    60001 gggccaccgc ctgcatccgg acgccgtact gataggcatg gatctggtgt gcgtcgcgcg
    60061 tcacgtggca gttcgtcagg aggcgctgcg gcgccaccag aacccccgag cccagcgaat
    60121 agccgccttg ctcccgcgat acctcgacct tgagcacctt ggtgctcaat tcgatgaacg
    60181 cagcccggtc gacacccgcc tgggcagtac cccaggaaca agcgactgcc agccacaacg
    60241 ctgcgtactt catggggcat cccggtctgg gcgagaactc tggagaaacc taattcgtca
    60301 acgcctgagg ggtgtcctcg atcaagggaa caccgaactc ctcgaggatc gcgcggatct
    60361 cgggacgctt cgcatcgatc agggattcga tctgctgctt ccactcgcgc tcaccatagc
    60421 gcacgcccat ggccatcttg aaatcgaact tgaccccggg ctcggagcgc atcgggaacg
    60481 tcatcagttc gggactcttg acccgcttcg cgaagaagcc ggcgatcggc ccccacacga
    60541 tggctgcatc gatcttgccg cttgccagat cgcgttcgat gatttctcct ggatagaact
    60601 cgggcgccgc gttcatcgtc gcataaggaa cgccttgctc gagcagcccg tgcttgacca
    60661 accaatcgga agcgggagtt cgatcgtaca gaccgatctt cagcttcccg agccgcgtgc
    60721 ggtcgacggc gaggaagtcc tcgacggact tgacttgatc caggccccgg ccctttgcga
    60781 tgaccagcgc ataagtggag tggtagtagg gtttcgtcgc cgacacctgg tcgtacccag
    60841 cgggtacgcc catcacgatg tcgcaacgga aatcctcacc cggcagcttg tagcgcaagg
    60901 tgttgcgtat gaatgccaga cgttgcggaa acgagtaata cgtgaccggc aggccgagct
    60961 ccttggcaaa caactcggcg atccgattct cgatgccatc acccccgaca ttggagaagg
    61021 gcaggttgtt cgggtcctga cagacgcgga aggcctggcg cgacggcttg gcctcatcct
    61081 gagcagtggc gtatggagac caccccagac cgagcgcgga ggtaatgcac attgcggccc
    61141 acaagcgcgt cgcccatcgg accggtgctc gtgccacttc aaccgggttg ctcattcgat
    61201 ggccgtcacg cgggcgctct tgatgtcccc gttcgagcgg cccttcaggt acgcgtacag
    61261 gtgatcgatg ttttccatga cgaccggact gttgccgaag ctttgcatgc ccttttcaag
    61321 ccggccattc gtcaccgtgg tcacgaattc ttccttggtc agaaccttca ggctgttgac
    61381 cagggacggg cccaccagac cttcctgatt ggcaccatgg caacggtcgc aggctgcggt
    61441 tcgccatgtc ttgaatcctt tcagggtctg agcatcgacc ttgtttccgt cgacaacggt
    61501 atacaggtcg gtcttgactt cttgcgccat gacaaggccc gatgcagcga aggcaagagc
    61561 tgcaagggca gcggtgatga gggtgcgtga cgtcatgatc ggaatgcttg ctcagctaat
    61621 tgaaaaggga tgacgccctg ctagagcgtc atcccctgga tcggttccct caccggttgt
    61681 ccggtgaggg ttaatcagga tgaatcccga tcagttgggg agcgcgaaca cggtcagcga
    61741 gccaccgagt tcggtgtact ggccgagttc cctgtagcca cccacagcac ccaggccgtc
    61801 ctgatccttc tcgagaccag ccgccatgcc gataccggcc cagccaccaa tgcccgagaa
    61861 cacgcccatg tactgcttgc ctttgtgttc gtaggtgaac acgttgccga tgatgccgga
    61921 cggggtcttg aacttgaaca gttccttgtt cacgtccttc ttgtcgacgc acttgaagta
    61981 gccttccagc gtgccgtagc aagacagacc acctgccgtg ttcagag
//
LOCUS       DAOORV010000007        58933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_578_length_58933_cov_8.674938, whole genome shotgun sequence.
ACCESSION   DAOORV010000007 DAOORV010000000
VERSION     DAOORV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 58933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_58933_cov_8.674938"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKV56_02005"
     CDS             <1..638
                     /locus_tag="PKV56_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOX66610.1"
                     /translation="QLHIHETSLYMVLYDSNTRHIFDDYTRFNSIVAELKELGTQDLI
                     RQGIPRDQIQHSLELDMRYGNQLVQTTAVIPQHEVHGPVDVLAIISQFSNDYGKRFGE
                     GSQAPEAGIRINTMRVAAFVQHETVEFEDIKPVAPNLRKSPPQPETVRKCHFVGHQGS
                     VETPVWSRSAIEPGVQISGPAIIASEVTTFLVDPGWTFVAARQGASWFLRV"
     gene            684..2960
                     /locus_tag="PKV56_02010"
     CDS             684..2960
                     /locus_tag="PKV56_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOX66611.1"
                     /translation="MTETVAPLAAKLPNAEEIALIKKFLNDTTLFLGPDPEIMKNHDL
                     MPRTALEEECIAQVSDAHTVAKIRDRIQAGCDEGFEMVEQMGAAPGAKWGDIITGVYS
                     ASGDLAIASAGGVLIFSALVHHPIKFIIKNWVNDPTVGVREGDGFIHNDSRYGNVHNT
                     DQSMIVPIFHKGQLVCWVASTVHEGENGAIEPGGMPSMAESPCDEGLKMCPFKVVENY
                     QIKRDILTFLQNSVREPKLQYEDMKVKLYACLRIKQRIEETLNTDGPAPLVSTLRLTM
                     ENVRAEVKRRISEWPDMTVRTYIIQDSTLRENCVVKVNCQLTKTGDRLIFDFRGSSPE
                     FTNRPTNTILAGLKGMLSQVILCYVWPDLPRGQAAFAPIEVITDPHSIVNSSYEAPNS
                     QSLMSIFTGFTAGQHAVAKFLYACPEKYTKVHAPTFNMINTFIWGGVSQHGETLGNLC
                     ADLNGMGGGARSDMDGEHALAPIFATMADIGEQELNEEDVPFLQLVSKKMTMDAVAPG
                     KYRGGQGYTMIVATKDSEQWGFMTTCQGAKIPPIQGLFGGYACGTYPLSKVKGVDVYD
                     VLMNEPHKFKHSIAEIMNERPFEDATYTTHHMGMGFEISKRGELFMISQGAGAGYGDL
                     LERDPAAVIKDIEEGLMSQGVAARLYKVKFDPVTLAIDQAATDALRADERKARIKRGV
                     PYKDFVKTWSKPKPPRTFQYFGCWGDDIGTLYVGSPDITRDANQPKPNYMRNPKDVRI
                     DELEARLAALGALKEDRQ"
     gene            2957..3826
                     /locus_tag="PKV56_02015"
     CDS             2957..3826
                     /locus_tag="PKV56_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66612.1"
                     /translation="MSVGFAEKLPDAAGQGPIRVWLKSSAYTRRVLLGASTDPWTSGA
                     QYLAYFSQAQGLLKPDVAVLEVGELYDAWNASHGDLAGAMGNRRKPATALRKLLEPED
                     PRKVLAEVVEAVLAHLRGQTPLVLSVPSPRRWMNHANRLTGRDDIEVTGDDVEDATMY
                     MADLIRSVSAHPVSGLLLEEGRDDVDFRVEDLTRYQSIINVVRHYRWSVALRLPGGAA
                     PSPEALADFDAVISPTLEQAGAAALGRDISASFAQDGAIAPRTGRQFYFAEIDPGLRP
                     ESVLEAVTRLRGG"
     gene            3819..5177
                     /locus_tag="PKV56_02020"
     CDS             3819..5177
                     /locus_tag="PKV56_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX66613.1"
                     /translation="MAESPRAPVDRIPVNLLTGFLGSGKTTLLNHWVQLPALAGSAVL
                     VNEFGQVGIDHHLVERVDEQMVLLDSGCLCCAMQGDLIGALKNLALRASRREIPPVTR
                     VIVETTGLADPVPVIYTLMEDPFVAARYVCDGVVTVVSATHGAQQLREFSEATRQVVA
                     ADLLFITKCDRATPSMLASLQDVLRELNPGASQRQVRDGKDDASALLACGVYSTADKP
                     SSLARWMGEEALRQGELRERGGGGRGSEVGPASDDGRFDAESSPDDDGIGRPLGSRLR
                     EQREARDADRRRSSASHTAGVASFVITFDQPVPWFGFAVALGQILSSHGSELLRVKGL
                     INVAGTVRPQVIQCVLGVAYPSVSLPAWPREGPFRDGRGRLVFITRGLDEAQVAAIRS
                     ALATLPGDAVALRMSAGDSMLPTRCWLSQRMPVTAPDALEHEGWFVQAKRFRRGGGPQ
                     SR"
     gene            complement(5197..5826)
                     /locus_tag="PKV56_02025"
     CDS             complement(5197..5826)
                     /locus_tag="PKV56_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66614.1"
                     /translation="MNCMKTTIWMGTAAGALVLAAAWPLPARADFELDDGKGKRILLK
                     DDGTWQYKNKAAEASAGAASEPAKEQPQADLLLERRIEAPGGCRFEMVLVNTLPYAIR
                     NLVPEFAVFRANGVEYRALTAGFGPVRPGDKGRRNMQFEGITCSEIARIQVRGGDRCD
                     MGDLQKFSEAVGECLARVRVQPSKLLTFEKASPELSAPSPLPALPDSKP"
     gene            6035..6976
                     /gene="ylqF"
                     /locus_tag="PKV56_02030"
     CDS             6035..6976
                     /gene="ylqF"
                     /locus_tag="PKV56_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOX66615.1"
                     /translation="MPVQWFPGHMNSTKKAIIERVKGIDVVIEMLDARLPASSGNPLL
                     AELTRGKPALKVLNKQDLADPQRTALWLQHFTEQPATRAVALDGRAGAPVHRIVEACR
                     ELAPLRGGMVKPLRVLICGIPNVGKSTVINSLAGRVAAKTGDEPGITKLEQKIVIASD
                     FYLFDTPGMLWPRIVVEQGGYNLAASGAVGRNAYDDEEVALVLLAALHRDYPGQVEQR
                     YKLDASVARTDDERLAAVGRARGAVLPGGRISLQKAAEIVLHDFRSGSLGRISLETPE
                     DWLRWQAEAAVKEAEREASRAARSKSRHASRSSPAEG"
     gene            complement(6994..7437)
                     /locus_tag="PKV56_02035"
     CDS             complement(6994..7437)
                     /locus_tag="PKV56_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66616.1"
                     /translation="MAQPEPDAPVEDLRGTVLCIEDDPVNRMLVEAMLSAFPAVTLRL
                     AVNGRDGIRAALEAMPDVILLDMNMPDMRGLDVVRVLSDRITDSACHVILLTAERFSI
                     DLAKAMSLGAREHWEKPLCADTVYGGLCRILRSQPNESTVGALNA"
     gene            7710..9902
                     /locus_tag="PKV56_02040"
     CDS             7710..9902
                     /locus_tag="PKV56_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX66617.1"
                     /translation="MSEHQHLDRHRELTRQFMQLLKSDPQRALSEALAGVQRTSSLGR
                     NDPQRADALEMLSHAHLMAEQYDKALPLAAEVVRIRKAGRPVDDELLALALDTHATVL
                     FANERSAESDAALRESLAAWRRAYGANDLRLAPILEGQAEYVQKAFGRPLWTIELLRE
                     AVAIRVQNVGSSGGKLAQTLSELAIHEMRQALYSDCDGHLAKAQELLEAEIRSDPARQ
                     EQKSGLIQVLVMRAGIAAVLGRQDLAFDQVQRARSIELTDRLQQAQAQLLIAESLSSA
                     LWLTGDLDGAIAAELESLDVFKNHQDLLESGALDKDLVGDTLTSLARLYLEKHELGLA
                     RQALKAARSGLGDTPDVLFNLSELERQSGNEALALAHYQAALRLRKESAAEIKVMFGT
                     NRAEERGGETGRFGTAVGDHLSLGESAVLVPGAQFSDVAWLRPARTVPLPVGLATDAT
                     RLLLRNKRVLDAAAFGVQARELMARARLYPKSALVFVHGFNVSFDEALQRAAQLARDL
                     NYDGAVFVYSWPSQGTVLRYGTDRDIADRAVDRFVEFLAQVELATGAEKINLIAHSMG
                     NRVLLPALAKIAADPQSRVRPKVREVILAAPAVPQADFTNWLDTISKQGIRRFTLYAS
                     SADKAMWAGWLREWATSLAGYASSGVPLWHPSVQSIDISKAAEPGLLQLNHDVFASNP
                     VMSEDIRQLLQQGQQRPPNQRMPMMKVQRSPDAGRAYWSYEPPAPARR"
     gene            complement(9961..10626)
                     /gene="leuE"
                     /locus_tag="PKV56_02045"
     CDS             complement(9961..10626)
                     /gene="leuE"
                     /locus_tag="PKV56_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HOX66618.1"
                     /translation="MSDWIHLQLGVTDFWSFVVGTVLIVLLPGPNSVYVLSVAAQRGV
                     RAGYRGACGVFLGDLILMLLSAGGVASLLRASPELFGLVKWVGGAYLGWIGLQMLRGA
                     WRLWQQRGHSATDRLPEPVTGDPFARALVISLLNPKAILFFVSFFIQFVDPTYPAPWW
                     SFGLLGLVCELTSCAYLTVLILVGARLAAEFRRRRRLSAGLTGAVGALFIGFGAKLAT
                     ASL"
     gene            complement(10623..12953)
                     /locus_tag="PKV56_02050"
     CDS             complement(10623..12953)
                     /locus_tag="PKV56_02050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOX66619.1"
                     /translation="MNVHVAARALCEFTDKAGDLDLRFTPSATALEGIAGHTIVSSRR
                     TQQGDGYEAEVGVCGEFDGLIVRGRADGYDPRRQRVEEVKTFRGDLDSMPANRRALHW
                     AQVQVYGALLCAERGLDEVELALVYFDVATQDETVLTERHSAQSLQQLFEDRCRRFRD
                     WAEQEAGHRRERDAGLEALRFPHADFRPGQRPLSEAVYRTAAAGRCLMAQAPTGIGKT
                     IGTLFPLLKACPDQQIDKVFFLTAKTPGRALALDALSVIRDSAPTLPLRVIELVARDK
                     SCEHTDKACHGDSCPLARGFYDRLPSARADAITRSVLDQTTLREVALAHHVCPYYLSQ
                     ELVRWSDVVIGDYNHWFDLSALLHGLTQAHEWRVGVLVDEAHNLIERGRQMYSASLRH
                     TDLRALLPKAPADVRPALRRLDRCWQAIESAQVDGEESVSYRVYPGPRQDLIAALQQV
                     VSVVTDHLAERPALDSPLLRFYFDALHFTRVHEDFGDHSLFDVTLDEPLDDSVLCLRN
                     VVPAPFLKPRFAASHTTTLFSATLNPVDYVSDLLGLPDDTACIDVASPFDAGQLRVDV
                     IDAVSTRFRHRADSLLPIVELMAVQFAEAPGNYLAFFSSFDYLQDVSGLLRTRHPEIT
                     LWEQSRAMNESARARFLAGFTPTSQGIGFAVLGGAFAEGIDLPGARLIGAFIATLGLP
                     QLNPVNEQIRERMEQMFGAGRGHDYAYLYPGVQKVVQAAGRVIRGPNDRGVVVLIDDR
                     YRQAQVRRLLPKWWSPRVRQCTARGGAPVAADEGQR"
     gene            complement(12976..14055)
                     /locus_tag="PKV56_02055"
     CDS             complement(12976..14055)
                     /locus_tag="PKV56_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX66620.1"
                     /translation="MPDRTALQAPPGAPITTEALVIGAGPTGLFQVFQLGLLEIACHV
                     VDSLAMPGGQCIELYPDKPIYDIPAVPVCTGRELVDRLLAQIAPMKPTFHLSQQVSAV
                     EPQADGGFEVTTTAGVRFRAGTVFIAGGVGSFVPRRIKVDGIDCFFGRQVIHSGEAVP
                     SAKGRRVIVIGDDEAALDTALALADSDIDRPASVTLMHRRDAFTASPATVDRMRAACA
                     AGRMQFVAAQAIGCESAGDTLTQLVVARADGTTLSLPVDLLLVLWGLSPKLGPIADWG
                     IQLDRKQVVVDTEKFETSVPRIFAVGDVNTYPGKKKLIVCGFHEATMAAYGAAAYLRP
                     DRPVQVQYTTTSTRLHRLLGVAPDA"
     gene            complement(14103..14429)
                     /locus_tag="PKV56_02060"
     CDS             complement(14103..14429)
                     /locus_tag="PKV56_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOX66621.1"
                     /translation="MTHVVTESCIRCKYTDCVDVCPVDCFREGPNFLTIDPDECIDCA
                     VCIPECPVNAIMPEEDVPGDQQHMIQLNADLARSGWPSITKRKPALPDADEWKDKTGK
                     LSELKR"
     gene            complement(14516..15340)
                     /gene="cobA"
                     /locus_tag="PKV56_02065"
     CDS             complement(14516..15340)
                     /gene="cobA"
                     /locus_tag="PKV56_02065"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOX66622.1"
                     /translation="MKAASGTTAFGRVAFVGAGPGQADLITVRGAQRLAQASVVLTDA
                     LADPALRELAPQARWLHVGKRGFADSTGQETINALLVRSAREVGAGGLVVRLKGGDPS
                     VFGRLEEELQAMAEAGIECEVVPGVTAALAAAASAKRPLTRRGSGRSVSFSTAMTRAA
                     PDHVAAEAASGPLRASRSADTEVFYMAGKQLGALARQLVRAGWPTDTEACVISRAGWP
                     DELHSDHTIETLGRASMLHAGRPTLVLVGAGARPIDAAQPPDTDHIPSPSKANLST"
     gene            complement(15337..16677)
                     /locus_tag="PKV56_02070"
     CDS             complement(15337..16677)
                     /locus_tag="PKV56_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX66623.1"
                     /translation="MSAVLQPADLRADPHEPLDATDHRALRFLTAGSVDDGKSTLIGR
                     LLFDSRAILADQLDTLEKRAAGAPIDLSLLTDGLEAEREQGITIDVAYRYFATKARKF
                     IIADAPGHEQYTRNMVTAAAGSDAAVVLVDITKLDTNQTPLVLLPQTRRHSLLAHLLR
                     VPSIVFAVNKLDAVADPRQAFDAVSVALRSFAEAAGIAVAGIVPVSALRGDNVTQTLV
                     TDGTDWSTWYVGPTLLQLLESLPAAQERTEGDLLIPVQYVAREGEGTGNQPRTLWGRI
                     AHGSVQAGDDVQIFPSGQRAVVAEVRLAGSVVDRVEAGRSAGVVLDRQLDVSRGDWIS
                     TPDSVTGLQSFAATLAWLDTEPAVVGRKYWVRHGNRWVQARIASIESRLDIHSLESID
                     AHELAVNEIGHVIIETQQPLPVEAYESNRSGGSLIVVDPTTNRTSGALLVKSAA"
     gene            complement(16677..17645)
                     /gene="cysD"
                     /locus_tag="PKV56_02075"
     CDS             complement(16677..17645)
                     /gene="cysD"
                     /locus_tag="PKV56_02075"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOX66624.1"
                     /translation="MNAAVSVEQLLPELDQTHLDWLEEEAIFILRETVAAFERPALLF
                     SGGKDSCVVLRLAEKAFKTKVDPNNPNEFKGRLPFPLLHVDTGHNFPEVIEFRDRRVA
                     EMGERLVVGHLEDSIKRGTVRLSHPLESRNGHQTVALLEAIEEHRFDVLMGGARRDEE
                     KARAKERIFSHRDSFGQWQPKEQRPELWTLFNTRHRPGEHFRSFPISNWTELDVWLYI
                     ARENIPLPGLYYAHQREVIRRKGLLVPLTDVTPPEAGETVETATVRFRTVGDMTCTCP
                     VESGAATAADIVAETLTVTVSERGATRMDDRTSDASMERRKKEGYF"
     gene            complement(17642..18409)
                     /locus_tag="PKV56_02080"
     CDS             complement(17642..18409)
                     /locus_tag="PKV56_02080"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOX66625.1"
                     /translation="MSAIDLYARKTAGFEERLAHTVVMLKEAAAAHGGRIVQSTSLGV
                     EDMVVTDLISRHGLGGAQGIAIATLDTGMLHAETLALIPRIEQRYGLAVEVFKPVNES
                     VVHFVATNGKDAMYQSLELRKACCGIRKMEPLARMMAGRSAWVTGLRREQSNTRAVVP
                     FSEDDGQGRTKFNPLADWTWNDVWHHVATNDVPYNPLHDEFMPSIGCAPCTRAIAVGE
                     DFRAGRWWWEDEKAKECGLHVKEEAPEGAVSMIGDRP"
     gene            complement(18406..18939)
                     /locus_tag="PKV56_02085"
     CDS             complement(18406..18939)
                     /locus_tag="PKV56_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HOX66626.1"
                     /translation="MKFIQYAQDPWHTVHGDDGPMVTITPKPHLLLTWLQWHSVCHHW
                     PAGMPVGVIVGNDLNVRELEADLPRFGLIALQFPKWVDGRAYSQAHLLRSRYGFAGEI
                     RAIGEVLVDMLPLLHRTGFDAVVLRADQSREAAERALTFFPGFYQGDVRERRPRFARA
                     DAGPESAAEFADGGAAI"
     gene            complement(18951..20672)
                     /locus_tag="PKV56_02090"
     CDS             complement(18951..20672)
                     /locus_tag="PKV56_02090"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOX66627.1"
                     /translation="MYTYTEFDKQFVRQRAAQYRDQLDRHLGGRLPDDEFRALRLQNG
                     WYVQRHAPMLRVAVPYGELSSAQLRGLARIARDYDRGYAHFTTRQNLQYNWIPLDKSA
                     EVMDQLAAVNMHGIQTSGNCIRNITSDCFAGIAPDESVDPRPYGEIMRQWSTLHPEFA
                     FLPRKFKIAVTGAGEDRAAIGWHDIGLHLLKNAAGEVGFKVLVGGGMGRTPVIGTVFR
                     EFLPWQQILVYIEAVVRVYNRYGRRDNIYKARIKILVKAEGQRFVDDVNEEFRNILER
                     DADGSAHVIPQSEFDRVNASFVVPAGVAARPSTGIPVAAGAPAGYTRWIERNVHRHRL
                     SGYRAVTLSLKRTGQAPGDITDAQMELAADLADRYSHGELRVSHDQNLVLPWVLETDL
                     PALWEAARSASFATPNIGLLTDMIACPGGDFCALANARSIPLAAEITERYQDLDELWD
                     IGDIDLHISGCINSCGHHHSGHIGILGVDKDGAEWYQITLGGSDGSVISGGPIAGKVI
                     GPSFAADEAADAVEAVIDTYQAQRQAGERFIDTVRRIGLDPFKAATNAVRRSTARDSA
                     TDAARAD"
     gene            complement(20706..21473)
                     /locus_tag="PKV56_02095"
     CDS             complement(20706..21473)
                     /locus_tag="PKV56_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOX66628.1"
                     /translation="MDPIAIFSGFGVGAIVGMTGVGGGSLMTPLLLSVFKLSPAVAIG
                     TDLWFAAITKIAGSASHHQAGHVEWRITRLLLAGSIPAALATIALMHFTGLTKGWASA
                     LTFSLGIALLLTAVTVAFKQIWVKVGHWLERWINDGNRPALTVLCGFILGVLVSLSSI
                     GAGAIGATLILLLYPKLASHRIVGTDIAHAVPLTLVAGIGHATLGNVDWSLLVALLVG
                     SIPGIWLGAQLTRKMPERLVRTLLCVSLVTAGIKVIH"
     gene            complement(21668..23140)
                     /locus_tag="PKV56_02100"
     CDS             complement(21668..23140)
                     /locus_tag="PKV56_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOX66629.1"
                     /translation="MRLSRTLIVTAIALLSNTCQVQAQSSGAASDPLRSAVEKALLSN
                     PEVASRFNAYRAAGDAVDVARGALRPRVDLNASVGIDKDKITSRQPESESLRRTGLGL
                     SLTQLLWDGLGTQREIDRVSHDRLSRYFDLLEATEQTTLEATRAHYDVLRFQRLVALA
                     EDNYVQHRYASQQIQSRFRAGVGRGVDLEQANARLALAESNLTTEVANLHDVMARYLR
                     VIGEAPPKAAPAGGAPTVLTTDLPASSTEVLNRAVLQSPAISASVEAVRAARTSAQVR
                     ESAFQPRIEARVRSGFGNNYEGVRDQKHDSSAELVLNWNLYSGGSDQARVRQQVNLVG
                     QAEDLRDKACRDVRQTAAIAFNDTRKLTEQLVLLDRNTLAIEKARDAYRQQFDIGQRS
                     LLDLLNSENELYTARRAYANAEYDRALAFARTHAGMSQLTAQLGIARPASLSEEAKDW
                     TVGEDAPGRCPADAITMQAIDFQALARRVPAVAEQVRPAK"
     gene            23429..24109
                     /locus_tag="PKV56_02105"
     CDS             23429..24109
                     /locus_tag="PKV56_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOX66630.1"
                     /translation="MPTVPRRSRYAAACWVLLGCLLSFASVPWAWDAERLSLAAAGHG
                     ARASAALRALQPLLAASPDPDERHRLQAINEFFNRRVQFRDDLEVWGQVDFWASPFEM
                     LEKGAGDCEDFAVAKYFSLISAGMAAQKLRLVYVRAQMGGPGGAIQAHMVLAYYATPN
                     AEPLILDNLITEVRPASRRPDLTPVFSFNADGLWQGVGAQAAGDPVARLSRWRDVMAK
                     ARAEGFEP"
     gene            24147..26072
                     /locus_tag="PKV56_02110"
     CDS             24147..26072
                     /locus_tag="PKV56_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX66631.1"
                     /translation="MSLIRQVWLLLLATVLLAFAGSVGVVTTSTRDLLQTQLRLKNSD
                     NAATLALALSQQKGDAELMSLLMTAQFDTGYYRQVRLLSPEGKVVFSRDAASKAAQAP
                     AWFVSAVPIESQPGVAQVSDGWRALGQVQVESQVSYAHDALWTSTLRSALAMALVGLL
                     AAALGMWVVRRISRPLDAAVAQANALEHGEFLTVPEPRMPEVRRLTRAMNSLVARLRL
                     SSGAQAEQIAALHRQATGDALTGLSNRTHFLVQLTAALQREDGPADSGLVLLRVMDLG
                     TLNRQFGHAVGDRILVTIAQSLKPYAEGVKGCFLGRLNGSDFALCLPAPGVALETAQA
                     VAEALRLVLPTLGSGAAVCLGAVEMHRDLSPGRLMSAADEALARAELRGAFAVELGSE
                     PPGAIAFLGEGAWRQRIVQALDQQRVRLMEFPVLNSRRQLVHLECPLRLQLESGGAFE
                     PAARWLPLAARSRLTVDIDERALTLALQAALHDGQPRCVNLSPASLGDSGFAARLRAR
                     LWAVPQAAKLVWLEIDESAAVEQFDLLRELARQLRSTGVRFGIQHAGERLGQIPRLFE
                     AGLDFVKLDASATIGVGRDDHRALFVRGTVTLLHGMAIQVYAEGVSDSRDASRLWDCG
                     IDGATGPWISTLDVAAG"
     gene            complement(26103..>28615)
                     /locus_tag="PKV56_02115"
     CDS             complement(26103..>28615)
                     /locus_tag="PKV56_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOX66632.1"
                     /translation="SDGQGGTDTATVNITVSPAPNLAPDAIDDSGSTAFNTPLTFVPA
                     DLLGNDTDPENNPLAITSVQDALHGSVSIVAGNVVFTPTAGYSGPASFTYTVSDGQGG
                     TDTATVNITVSPAPNLAPDATPGSVSGDEDTLLPVSLTGTDADGTVQSVTVTTLPSNG
                     TLFLHDGTTPVTVGTPLSPADAAQLVFKPNPDFNGAAEVVFTVTDNDNLTSAPATFTL
                     DVVPINDPPLAQNDEGTVQASQTRTVDAAEGIILSTSVDAGRDSDPEGDSLTVMQVVA
                     GTGAPSTAVSPAGVTVAGIYGDLLLRSDGSYDYIANRAGSIATGSQADDVFTYEVADG
                     NGGLSTATLTLHVAGEGDTVTADPPTINPLANPLGLSGEYYGYNDFNPNGTNSNRRHG
                     DDRTLGNLDHVSDINAIVNGRNAAFGGGNVVGTTTASNENATDARFTSTAIDYGVTQT
                     VTNTLGTNPNLAAGASIAALNNDNSQLFRLLNRAVGSDAGSLRITQGTGDSDTSGTGP
                     TSGLGKTSDAAIRLTGQAYLAAGMYDIRVTADDGFRLRLGNHDVAIFDGIQSPTTRVY
                     TGVPIDGGMTPLELIYWEQGINAVLRVEFKPSGSPDSGYAILGSQNVPLFSDAHAPAL
                     TDLQDIVAGAAPGTYNIRTGSVLDGGGGNDDLTGNAGRDRLIGGLGDDTLNGGGGADL
                     IIGGQGDDQMTGGAGDDVFRWSLGDGGSVTTPAHDVIADFDNRNHAGDVLDLRDLLVG
                     ESHAANGVALPASIGLNNAITVTPNEGNLANFLHFSVSGGDTVVEISSTGGFAGGTYN
                     PAAVDQVITLSGMNLVGGFSNDHQVINDLLQRGKLVTD"
     assembly_gap    28616..28715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28716..30134)
                     /locus_tag="PKV56_02120"
     CDS             complement(<28716..30134)
                     /locus_tag="PKV56_02120"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOX66633.1"
                     /translation="MPTILQTAQGRVTGLWGSALIKGPDGRMRALKMGDLVQRGDVIL
                     TTQDGIVQLTQDEAQVTAAPIKAAPAPELDRVISDLNQGDPDAAPGAGLNGGDGGEFL
                     PGLRVERIQEGITPAGLAFSSNSAAGITAEAGTGATVNNIAPEALDDAVSGNEDTTLP
                     VPLIGNDGDGSVASVTVTSIPPGSTLLLADGTTVVMAGQTLTPGQAAGLLLQPSANFN
                     GQTAITFFVTDNEGLASNPATVTINVISVNDAPVAAPFSAIGPEDTPLPVSLSASDVD
                     GTVTSFTISRLPTNGTLFLSDGTTPVIAGVPLTPTEAANLVFVPAPNFFGTVNLTFTV
                     TDNEGLPSAPTTVPLIITPVNDGPDAVNDSASTAHNTSVSVSVLANDTDPEGDPLTVT
                     SATMVNPAQGSVTVAPNGTLLFTPASGFSGTALINYAISDGQGGTDTATVTINVAAAP
                     PPPTGPDAIDDSGSTAFNTPLTF"
     gene            complement(30364..31725)
                     /locus_tag="PKV56_02125"
     CDS             complement(30364..31725)
                     /locus_tag="PKV56_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOX66634.1"
                     /translation="MKAIAKVASARWGATDAAADAAGFGSFEQQADVVMSRARTQRAQ
                     LIVRAAVVVLLLLVLWACFAQVEEVTRGDGKVIPSKQLQIVQSLDGGVVSEILVQEGQ
                     VVEAGQLLLKIDETRATSGVRESAAQGFALRARQARLRALAEGSAFKPPTSTNAIEGR
                     ILDEERTLYETRRSELGTMIAINQQQLQQRQQELSEMKARKSSAERGLDLGQQELNKT
                     RPLLATGAVSEVDILRLDREVSRSRGEIEQATAQIARVQAAIGEAQRKIQETELTFRN
                     EARKEMADVMGKLNALNEGAVALADKVDKSQVKSPVRGRIQRLLANTVGGVVQPGKDI
                     VEVVPLDDQLILEAKVQPRDIAFIHPGQDATVKFTAYDFSIYGGLVAKVENISPDTVV
                     DEKGNAFYLVRVRTTQANFSEKLPVIPGMTAEVDILTGRKTVMSYLLKPVLKAKAYAL
                     RER"
     gene            complement(31722..33872)
                     /locus_tag="PKV56_02130"
     CDS             complement(31722..33872)
                     /locus_tag="PKV56_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOX66635.1"
                     /translation="MQRLREDLIHPDALLDCLVEICRLQGHPTTRAALSAGLPIGADT
                     QGRLPLDLVERAAARAGMATRLQRQALDGIDSVTLPVLLILHENQACVLLGWDGDEAR
                     VLLPETGQGAVRLARTELAARYSGVVLFVRPHFRFDAGAQTPPERTATEQHWFWSALL
                     AQRFVYRDVLWAALLINLFALVFPMFSMNIYDRVVPNNAFETLWSLSIGVLLVLGADL
                     FMRLLRSHFVDEASARIDVALSAKLMEKVLGLRLEHRPESVGSFAQNLRGFEQVRDFI
                     ASSTVTALIDLPFALLFVVVLAWISPWLAVPVLVAFSIILVLGYFLQHRLHALSETTY
                     QASAQRNATLIESLSAIETIKSQGAESVIQSKWERANLFLAGTNVRMRGLSSGAMYAT
                     STLTQLVAVSMIVIGVYLISQRELTMGALIAASMLSGRALAPAGQIVGLLMQYQGART
                     ALDSLNKIMAKPVERPANENFIQRPQLRGDIEFRNVRFAYPGRTDSAIEGISFRIAAG
                     ERVALIGRVGSGKSTIQRLIMGLYQPTDGAVLLDGIDLRQLDPADVRRNLGSVSQDVT
                     LFHGTLRENITFGLPYADDSAVVAAAEVAGLTEFINRHPRGFDMPVGERGESLSGGQR
                     QGVGLARAVLHNAPLLLLDEPTSAMDFSTEAQITAKVTAFASDKTVVLVTHRTSMLAM
                     ATRVIVIDGGKVVADGPRDRIMEALSTGRIARAA"
     gene            complement(33973..34518)
                     /locus_tag="PKV56_02135"
     CDS             complement(33973..34518)
                     /locus_tag="PKV56_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOX66636.1"
                     /translation="MHPTRSIRHCKTCGSAAEYRVPADDNRERAVCTVCATVHYENPC
                     NIVGTVPAWGDQVLLCRRNIEPRYGYWTLPAGFMELNETLAEGAVRETVEEAGANIEL
                     QPLFSLLNVVRAGQVHLYYRARLLDTRFAPGPETIEAQLFHEHEIPWEDLAFRTVRET
                     LKLYFEDHRRGQFGFHCADIG"
     gene            34619..35401
                     /gene="pgeF"
                     /locus_tag="PKV56_02140"
     CDS             34619..35401
                     /gene="pgeF"
                     /locus_tag="PKV56_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOX66637.1"
                     /translation="MAFHPDWLRPPWKTPGVSAVMTTRRGGVSAGPYASMNVRDGVGD
                     EPECTARNRALLTEAIGAAPTCLDQVHGLGVARLTARDSAPHAPRHEADACISTEPGV
                     VCAVQVADCLPVLFAAPGVVAAAHAGWRGLAAGVLEATLDALCVAGGCAPDQVEAWLG
                     PCIGPDCFEVGADVLSAFGVDPSAPEDVPGRGRAAARFRAVNQGKWLADLPALARDRL
                     TSAGVGSISGGSRCTVSDPSRFFSYRRDGVTGRMVAAIWLTG"
     gene            complement(35330..35620)
                     /locus_tag="PKV56_02145"
     CDS             complement(35330..35620)
                     /locus_tag="PKV56_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66638.1"
                     /translation="MYLVAIAWMYVVVMMTVVEATSPNGTIIGAVSTFLLYGAFPLAI
                     VLYILRTPARRRARQLAEASATLGSTNEASTREPDGGNHPAGDTVPTVGKEP"
     gene            35859..37508
                     /gene="phaC"
                     /locus_tag="PKV56_02150"
     CDS             35859..37508
                     /gene="phaC"
                     /locus_tag="PKV56_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOX66639.1"
                     /translation="MTQLTLPVPTIAQLQTDYIQQATALWNQSLQQFQAFAGSALDQS
                     AAAAEKPVLADRRFAAPDWVSNPASAFTAQFYLLNARTLMNMADQLQGDEKTKQRIRF
                     AVQQWVDAASPSNYLALNPEAQRKALETKGESIKQGLQHLWNDIQQGHVSQTDESLFE
                     VGRNVATSEGSVVFENELFQLLEYKPLTAKVHERPMLFVPPCINKYYILDLQPDNSLI
                     RYTVEQGHRVFVMSWRNADESMAQFTWDRYIEDGVIRGIEVARDISGADQVNTLGFCI
                     GGTLLSTALAVLAARGEKPAASMTLLTTLLDFSHTGILDLFIDESMVRMRETSIGLDS
                     PAAGGLLKGQELASTFSFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDSTNLPGPM
                     YCWYLRNTYLENRLMKPGDLKVCGVPLDLSLIDTPAYIYASREDHIVPWDAAYRSTQV
                     LAGKGKKRFVLGASGHIAGVINPPAKKKRSHWVGGTAALPVGADAWLDKAREQPGSWW
                     TDWAQWLASQAGRQIPAPKSAGNRRHPVIEPAPGRYVKQKA"
     gene            37591..38772
                     /locus_tag="PKV56_02155"
     CDS             37591..38772
                     /locus_tag="PKV56_02155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOX66640.1"
                     /translation="MSTDIVIVSAARTGVGKFGGSLAKVSASALGSVVIAEVLKRAGL
                     TGEHVSEVIMGQVLQAGTGQNPARQASIGAGLPNTVPAMTINKLCGSGLKSVALAVQA
                     IRDNDAEIVVAGGQENMSLAPHVLPGSRDGVRMGDWKLLDTMLSDGLTDAFNLYHMGV
                     TAENVAKKYGISREAQDAFALSSQMKAAAAQEAGRFNDEIVNVSIPQRKGDPVLFGTD
                     EFVNRKCSAEGLAGLRAAFDKAGSVTAGNASGINDGAAAVLVMSAARAAALGLTPMAR
                     IASYASSGLDPAIMGMGPVSASQRALARAGWKASDLDLMEVNEAFAAQACAVHQEMGW
                     DTSKINVNGGAIAIGHPIGASGCRVLVTLLHEMQKRNAKKGLASLCIGGGMGIALTLE
                     R"
     gene            38877..39614
                     /gene="phbB"
                     /locus_tag="PKV56_02160"
     CDS             38877..39614
                     /gene="phbB"
                     /locus_tag="PKV56_02160"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOX66641.1"
                     /translation="MSKKVAYVTGGMGGIGTTMCQRLHRDGFTVIAGCGPSRDFKKWL
                     DEQSALGYTFHASVGNVGDWNSTVAAFQQVKAEHGPIDVLVNNAGITRDGMFRKMSRA
                     DWDAVIETNLTSLFNVTKQVIEDMLDKGWGRIINISSVNGEKGQFGQTNYSAAKAGMH
                     GFTMALAQEVANKGVTVNTVSPGYIATDMVKSIRQDVLDKIIAGIPVKRLGTPDDIAS
                     MVSWVASDESGFATGADFSVNGGLHMG"
     gene            complement(39636..40691)
                     /gene="mnmH"
                     /locus_tag="PKV56_02165"
     CDS             complement(39636..40691)
                     /gene="mnmH"
                     /locus_tag="PKV56_02165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOX66642.1"
                     /translation="MALTPVTADDCLARLDAYDAIIDARSEGEFALDRIPGAVNWPSL
                     NDAERALVGTEYKQISPFVARKRGAALVARNIAMHIERHVLDKPRDWQPLVYCWRGGQ
                     RSGSLALVLSKIGFRVQVLEGGYQAYRRAVMKALEEQPLALRFRAICGRTGSGKSRLL
                     AALQAQGAQVLDLEGLANHRGSVLGLVPGSPQPSQKQFDSRLWDAMRRFDPARPVWVE
                     SESRKVGELRVPERLIECMRASPCVHIQLPVDARVSLLMEDYAFFVDDVPTFCTRLDA
                     LRALRGNEVINGWQEAARSGGIEGVVRELLLTHYDPVYLQSMQRNFIGFADARLVEPE
                     NGDASTLAGVARDLLQD"
     gene            complement(40731..43376)
                     /locus_tag="PKV56_02170"
     CDS             complement(40731..43376)
                     /locus_tag="PKV56_02170"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOX66643.1"
                     /translation="MSVVELDEHGALAPDCLGELRQRYKAGKDALIRHFEASRPTAPA
                     ATRLIRGIARHTDVTVLNLWELCGMPAEAALLAVGGYGRGELFPHSDVDVLVLLPSGL
                     CASGSPQSEAKRSVESFITACWDIGLEIGSSVRTVEECMAEAERDVTVRTALLESRFI
                     CGSRRGFNVFRRNNSQAMQPRNFLRAKVLEMRQRHQKFEDTPYSLEPNCKESPGGLRD
                     LQLIIWVARAAGLGRTWAELAASGLATPFETKQLQRNEGLLKLIRTKLHLVAGRREDR
                     LVFDLQNAVAESFGYRATKGQRASEALMRRYYWAAKAVVQLNQILMLNIEDRITGSQD
                     APMRPINEDFLERDGMLEVVSDDLYQRRPEAILETFLTYQTTRGITGLSARTLRALYN
                     SRDLMNAEFRRAPANRATFMRILRAPSGHTHALRLMNETSVLGRYLWVFRRIVGQMQH
                     DLFHVYTVDQHILMVLRNVRRFFIPEHVHEYPFCSQLALQWDKPWVLFIAAVFHDVAK
                     GRGGDHSELGASEARRFCRDHGIERDDTALIEFLVQAHLTMSRVAQKEDLSDPAVIQE
                     FAQRVGTERRLTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRASLRALGGAQPHLDA
                     EIDARKQEARHMLNLALQPPDVEVPLWKTLAISYFARHDVGEIAWHARSLHEHLDAAT
                     PVVKARVSPVGEGLQVVVYARDRADLFARICGYFDSAHFNILDAKVHTTRAGCALDTF
                     QVINPQLEASRDGAPAAQYRDLILLVETQLGRALASDGALPEPSRGRLSRRVKSFPVT
                     PRITLRPDERAQNWLLGVSASDRSGLLYAIARVLARHQINLQLAKITTLGERVEDTFL
                     IDGAALRHNRTQIEIETELLDAIAA"
     gene            complement(43389..44204)
                     /gene="map"
                     /locus_tag="PKV56_02175"
     CDS             complement(43389..44204)
                     /gene="map"
                     /locus_tag="PKV56_02175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOX66644.1"
                     /translation="MTITIKTADDIDGMRIAGRLASEVLDMLEPHVKPGITTEALDKL
                     AHDYMVNVQQAIPAPLNYAPPGYTPYPKSICTSINHQVCHGVPNSKPLKNGDIVNVDI
                     TVIKDGWHGDTSRMFVVGEGSIAARRLCQMTYDAMWRGIAKVRPGARLGDIGHAIQTF
                     AENNGFSVVREFCGHGIGRKFHEDPQVLHYGKPGTLEVLTPGMIFTIEPMINAGRREI
                     REAGDGWTIVTKDRSLSAQWEHTVLVTETGYEVLTLSAGSPAVPQFVNESVAA"
     gene            44329..48369
                     /gene="purL"
                     /locus_tag="PKV56_02180"
                     /gene_synonym="purI"
     CDS             44329..48369
                     /gene="purL"
                     /locus_tag="PKV56_02180"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOX66645.1"
                     /translation="MITSPKHLLHVEGGSALPAFRAQALLAKLQQDASCGGARITAVH
                     ARHVHWVWSEAPLDPDQRDTVDGLLRYGDAYGGPSEGALIVIAPRLGTVSPWASKATD
                     IAHNCGLAIKRVERVTEYRLTLKTGLLGGSKPLTVDELKAAAELLHDRMTESVLLCRE
                     DAAHLFDERPAAPMAQVDVLGQGRAALVTANVDFGLALSDDEIDYLVSAFTSLRRNPT
                     DVELMMFAQANSEHCRHKIFNADFTIDGAVQDRSMFQMIRNTHQLAPQHTVIAYSDNS
                     SVMEGGVIERWLPEGYTDAPRYGVRSELAHVLMKVETHNHPTAISPFPGASTGAGGEI
                     RDEGATGRGSKPKAGLTGFTVSNLNLPGTDWPWERSPYGKPEHIASPLQIMIEGPLGG
                     AAFNNEFGRPNLAGYFRVYEQTVGVPGHQQRRGYHKPIMIAGGLGTIAATQTDKVEFA
                     AGTLLVQLGGPGMRIGMGGSAASSMAAGANAAALDFDSVQRGNPEIQRRAQEVINHCA
                     ALGDANPILAIHDVGAGGISNAFPELVDGAHRGARFDLRQVPLEESGMSPKEIWSNES
                     QERYVLAVQPDRLPMFDAMCLRERCPYAVVGVATEAKQLVLSDEPVTSVRAPSLLAID
                     MPMDVLLGKPPKMHRDVKRVRPQLGALDLTDVDLQTVALDVLRHPTVASKRFLITIGD
                     RTVGGLSHRDPMVGPWQVPVADCAVTLADYSGFRGEAMSIGERTPLAAIDAPASGRMA
                     VGEALTNLLAAPFALERVKLSCNWMAACGEPGEDAALYDTVRAVGMELCPALGISVPV
                     GKDSLSMRTRWSDRGESRQVTAPVSLIVSAFVTLDDVRGTLTPQLQPGDTTLILIDLG
                     NGRNRMAGSMLAQVLDQTGNASADGVPDLDDPAQLKSLIAAINQLRSEGRLLAYHDRS
                     DGGLWATVCEMAFAGHLGVSLNVDMLVTEGDGISDSRMDCGDSKNWAGQIGARRHELT
                     LKALFSEELGAVIQVPTAVRNEVMQTLRAHGLSKHSHFIGKPNDAGSVEVWRDTKAVF
                     SAPLRVLHQVWDEVSWRIARLRDNPHCADAEHDSAGLAEDVGLQVHLTFDPAQDVSAT
                     FIHTARPRLAILREQGVNSQVEMSYALAQAGFDTYDVHMSDLQAGRARLDQFIGFVAC
                     GGFSYGDTLGAGEGWARSILFNSKLADQFAAFFGRTDTFALGVCNGCQMMAALSPIIP
                     GAQAWPKFTRNKSEQFEARLSLVEVMDSPSIFFSGMAGSRLPVAVSHGEGRADFSQRG
                     DASAVHRAMRFVDHAGHATEAYPFNPNGSPDGLTSVTTADGRYTVLMPHAERVFRNVQ
                     ISWTSGPASESSPWARMFRNARRWVA"
     gene            48425..48895
                     /locus_tag="PKV56_02185"
     CDS             48425..48895
                     /locus_tag="PKV56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOX66646.1"
                     /translation="MRSFFQILVAALALAGCAQLPMSDPVKVSLVGIEPMPGQGLELR
                     MAVKLRVQNPNESEVAYNGVALDVELRGLSFASGVSDVQGTIPRYGEAVLVVPVSVSA
                     ISVLRQALSFANGDRSNVGYAISGKIGGSLLRGERFKSTGQIDLPTSLLGPPGR"
     gene            48929..49693
                     /locus_tag="PKV56_02190"
     CDS             48929..49693
                     /locus_tag="PKV56_02190"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOX66647.1"
                     /translation="MREAIAVGEERFERLDALRGLAIVWMALFHLCFDLNHFGFIRQD
                     FHNEPLWTVQRSAIVGLFLFCVGLSQAVALDGGHDRSAWGGRFWLRWAQIAGCALLVS
                     LGSWWMFPRSYISFGVLHGIAVILLLLRLAAPAGRWLWLFGLAALLLPLWFQHEFFDS
                     RWTNWIGLVTARPITEDYVPLLPWLGVSLWGLAVGQWVLQHRPTLLSLPLSPTLRPAA
                     RPLACLGRWSLSFYMVHQPVLIGLVAAAAWLVRTPA"
     gene            49735..50496
                     /gene="rlmB"
                     /locus_tag="PKV56_02195"
     CDS             49735..50496
                     /gene="rlmB"
                     /locus_tag="PKV56_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOX66648.1"
                     /translation="MSNKILFGFHAVTVRLKTTPKSVVEIHVDTSRRDQRMRQFIERA
                     TAAGSKLIDSDDAKLQKMCGTHRHQGLVARVEPVALSRSLDDTLDELDAVEGNPLLLV
                     LDGITDPHNLGACLRVADGAGAHAVIAPKDHAVGVNATVSKVASGAAETVPYFMVTNL
                     ARTLNELKERNIRVIGTSDTATQSLYDIDLTGPVALVLGAEGAGMRQLTTKTCDELVS
                     LPMRGAVESLNVSVASGVCLYEALRQRRAAEGNLA"
     gene            complement(50528..51430)
                     /gene="egtD"
                     /locus_tag="PKV56_02200"
     CDS             complement(50528..51430)
                     /gene="egtD"
                     /locus_tag="PKV56_02200"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOX66649.1"
                     /translation="MHRELLTGLSAEPATLSPKFLYDRLGSLLFEAITELPEYYPTRT
                     EARIFADHAPEMAAAIGPIGTLIDLGAGNCAKARMVIPPLRPSRYVAVDISVDFLEPA
                     IAQVQREHPAIEVCGVGLDFSSTVQLPPGLLQGRAVFFYPGSSIGNFTPASAQGFLAG
                     VRRQATGGGLLIGIDLVKPIEQLEAAYDDALGVTAAFNLNLLRHVNRLIGSNFDPRDW
                     RHVSFFNRDHSRIEMHLEARRAVVVQWPENERRFEAGDRMHTENSYKYTVESFAELLH
                     DSGYARTRCWVDANGWFAVFWAQA"
     gene            51692..52516
                     /gene="lepB"
                     /locus_tag="PKV56_02205"
     CDS             51692..52516
                     /gene="lepB"
                     /locus_tag="PKV56_02205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOX66650.1"
                     /translation="MQIGLSINEFLFCAMLATGLCRCAWRWKVRAMPEPPKRQPFWAM
                     LGADLFVVVALVFTCRVAVADWPRVPSESMEPTLRVGDYLLINHLAYGPRLPFTNTAI
                     EFASPQRGDVVVFRYPHDVSQFYVKRLVGMPGDVVTFADGTVSIQGAMPDAQVLEDDA
                     LAAFSIRRSADDRGQWMVRESAGAPPRVLKINPFVRGRQPLNWDDAGIAAQCSSTRGG
                     AWECTVPEGHYLMLGDNRDASSDSRTWGFLSHDQVYGKAVRVLFNFDDPERAWLPL"
     gene            52536..53810
                     /gene="coaBC"
                     /locus_tag="PKV56_02210"
     CDS             52536..53810
                     /gene="coaBC"
                     /locus_tag="PKV56_02210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOX66651.1"
                     /translation="MIDTPDPDADLRGKHIVLGLSGGIACYKAAELARALVKAGATVQ
                     VVMTEAAEQFITAVTMQALTGRAVFTSTWDARAVDGSANNMAHINLTRQADAVLVAPA
                     SADFMAKLLHGRADDLLSLLCLARPIESCPLLIAPAMNREMWSHPATQRNVVQLRADG
                     AHVLGPASGEQACGEVGDGRMLEPEELLEELTAFFRPKPLAGKHVLITAGPTFEPIDP
                     VRGITNLSSGKMGFAIARAAAEAGAEVTLVAGPVHLTTPRGVRRIDVRTAMQMFDAVV
                     PAAANQDVFIATAAVADWRPASLSEQKIKKNGSKIAPIFELTENPDILAAVASQALAR
                     GRPFCVGFAAESHDLLKHSREKLVRKQVPLIVGNLGPATFGRDDNALVLVDADGHRDL
                     PQADKLTLARWLIADIALRLAHVPDGKTGKPQ"
     gene            53807..54256
                     /gene="dut"
                     /locus_tag="PKV56_02215"
     CDS             53807..54256
                     /gene="dut"
                     /locus_tag="PKV56_02215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOX66652.1"
                     /translation="MTTIDVKLLDARMAEAMPAYATPGSAGLDLRACLDEPLLIEPGQ
                     TKLIPTGLAVHIGDPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRG
                     VASYTVQPMERIAQLVIVPVVQASFRRVDEFGASERGTGGFGSTGAN"
     gene            complement(54288..54809)
                     /locus_tag="PKV56_02220"
     CDS             complement(54288..54809)
                     /locus_tag="PKV56_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX66653.1"
                     /translation="MEIQSPCVVALTWRLEDAQGQLIDELAQPVEFFYGGADLLEKVE
                     ASLLGQATGFEASLHLEPEHAFGDYIADLVFFEERSVLPDGIEVGMQFEGVPEGSVTP
                     GMPEDAVYTVTEVYPTHVVLDGNHPLAGVALRLHLTVQDVRQATSDEIDAGSVGQPAF
                     SVFNADPPGPHLH"
     gene            54889..56019
                     /locus_tag="PKV56_02225"
     CDS             54889..56019
                     /locus_tag="PKV56_02225"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX66654.1"
                     /translation="MRIDHPTPLLGGLSPQAFMRRYWQKKPLLIRQAVPEGVAELDRA
                     ALFALAAADGVESRLIERRDTGWKLRHGPFARRQLPPLGRPGWTLLVQGVDLHAQAAH
                     RLMSRFRFAPDVRLDDVMISFATDGGGVGPHFDSYDVFLLQLAGRRRWRVGRMKNPTL
                     QPDVPLKILSHFEPELEWTLEPGDMLYLPPRWAHDGVADGDCLTASIGFRVPSREEVG
                     CEVLQRMLDAVDEREEGLRYRDPTQPATEQPARIPQPLADFAADAVRRLVDDPDSLAC
                     ALGEWLSEPKPQTWFDPVDEWSGSGTVVLDRRTRMLYDERHVFINGESFIASGRDAKL
                     MRVLADRRSLDAKSVARLGADARELLQSWTDQGWLHAADIAD"
     gene            56081..56569
                     /locus_tag="PKV56_02230"
     CDS             56081..56569
                     /locus_tag="PKV56_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66655.1"
                     /translation="MSTPASHLIQSRTEFQDALRRAFADIAAAGAREIWMCDADFADW
                     PLNEPEVVENLSRWAMGHRTCTIVALSYDQIVRRHPRWVQWRRERSHMVRCRTPDEAD
                     RVDVPCVLLAPGTLSLRLVDRDLYRGSVSTAIADATRDRDRLDALMQRSVDAFPASTL
                     GL"
     gene            56712..57005
                     /locus_tag="PKV56_02235"
     CDS             56712..57005
                     /locus_tag="PKV56_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66656.1"
                     /translation="MNKTLVLATLVAAVALAACGKKEEMAPAMEAASAAAAPAVAAVE
                     AAAASAVDAAASAAEATVGAASGAATEAAGAAADAAKDAANKAADAATDAMKK"
     gene            complement(57103..58368)
                     /gene="bamC"
                     /locus_tag="PKV56_02240"
     CDS             complement(57103..58368)
                     /gene="bamC"
                     /locus_tag="PKV56_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOX66657.1"
                     /translation="MHRRQRKAPGSALPSPTAINGLRGMSAANPTEMNNVTASQPTRS
                     NRLALRRSNARRLACSALVIGMAGCSSVDTLMHGDKVDYKASAATATAGLEIPPDLTQ
                     LSRESRYRQDDSGAFSAAAYQSSPSNLSTGAAVAPAGDAALRIDRAGNERWLVTTMTP
                     EQLWPVLQEFWRERGFNLTVNQADVGVMETEWNENRARLPQDFIRNTIGKVFDAIYST
                     GERDKYRTRVERSAGGSDIFISHRGMVEVATGALKDSTVWQPRPADPELEAEMLRRLL
                     LKLGAKEDQAKAMMATTEPQAPRARVVAGQTSVIEVDEGFDRAWRRVGVALDRTGFTV
                     EDRDRIQGIYYVRYADPKPYNKEEPGFFGKLLGFGKDKNDPDKLARYRVQVKGEGERS
                     TVSVLTEKAGTEKGDTPARIATLLVNDLK"
     gene            complement(58379..>58933)
                     /gene="dapA"
                     /locus_tag="PKV56_02245"
     CDS             complement(58379..>58933)
                     /gene="dapA"
                     /locus_tag="PKV56_02245"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOX66658.1"
                     /translation="NKPSQEGIYQHFKAIAEAVDLPLVLYNVPGRTVADMQPETTLRL
                     AQLPGIVAVKEATGNIERAAWLIKHAPPGFSIYSGDDPTAIALMLLGGHGNVSVTANV
                     APRSMHELCVAAMEGRVREATEIHMRLLPLHKHLFTEPSPAPTKWALAQLGRCRNTLR
                     LPLMPLTAAGQATVESALRDAGLI"
BASE COUNT         9647 a        19045 c        19892 g        10249 t
ORIGIN      
        1 accagctgca catccacgag acgtcgctgt acatggtgct gtacgactcg aacacgcgcc
       61 acatattcga cgactacacg cgcttcaaca gcatcgtggc cgagctgaag gaactgggca
      121 cgcaggatct gatccggcag ggcatcccac gagaccagat ccagcacagc ctcgaactcg
      181 acatgcgcta cggcaaccag ctggtgcaga ccaccgcggt gattccgcag cacgaagtgc
      241 atggccccgt cgacgtgctg gcgatcattt cccagttctc caacgactac ggcaagcgct
      301 tcggcgaagg cagccaggcc cccgaggccg ggattcgcat caacacgatg cgggtggccg
      361 ccttcgtgca gcacgagacc gtggagttcg aggacatcaa gccggtagcc ccgaacctgc
      421 gcaagtcacc accccagccc gagacggtgc gcaagtgcca tttcgtgggc catcagggct
      481 cggtcgagac cccggtgtgg agccgctccg ccatcgagcc gggtgtccag atctccgggc
      541 cggccatcat cgcttcggaa gtgaccacct tcttggtcga cccgggctgg accttcgtgg
      601 cggcacggca aggcgcctct tggttcctgc gtgtctgacg acacgcggga cacacgccga
      661 accgacaaac aaagaggatt gacatgaccg aaaccgtagc gcccctggcg gccaagctgc
      721 ccaacgctga agaaatcgcg ttgatcaaga agttcctgaa cgacaccacg ctgttcctcg
      781 ggcccgatcc cgagatcatg aagaaccatg atctgatgcc gagaactgca ctcgaggaag
      841 agtgcattgc gcaggtcagt gatgcccaca ccgtggccaa gatccgcgat cgcatccagg
      901 ccggttgcga cgaaggcttc gagatggtgg agcagatggg cgctgcgccc ggcgccaagt
      961 ggggcgacat catcaccggc gtgtactccg cttcgggcga cctggcgatt gccagcgccg
     1021 gtggcgtgct gatcttctcg gccctggtgc accacccgat caagttcatc atcaagaact
     1081 gggtcaacga cccgaccgtg ggcgtgcgcg agggcgacgg cttcatccac aacgattcgc
     1141 gctacggcaa cgtgcacaac accgatcaga gcatgatcgt gccgatcttc cacaagggcc
     1201 agctggtgtg ctgggtggca tcgacggtgc acgagggcga gaacggagcc atcgagcctg
     1261 gcggcatgcc gtcgatggcc gaaagcccgt gcgacgaagg gctcaagatg tgcccgttca
     1321 aggtcgtcga gaactaccag atcaagcgcg acatcctgac cttcctgcag aactcggtgc
     1381 gcgagcccaa gctgcagtac gaggacatga aggtcaagct ctacgcttgc ctacgcatca
     1441 agcagcgcat cgaggagacg ctcaacaccg acggtcccgc gccgctggtt tcgacactgc
     1501 ggctgacgat ggaaaacgtg cgtgccgagg tcaagcgccg catcagcgag tggcccgaca
     1561 tgacggtgcg cacctacatc atccaggact cgaccctgcg cgagaactgc gtggtcaagg
     1621 tgaactgcca gctgaccaag accggcgaca ggctgatctt cgacttccgc ggctccagcc
     1681 cggagttcac caaccggccc accaacacca tcctggccgg tctgaagggg atgctgtcgc
     1741 aggtgatctt gtgctacgtg tggcccgatc tgccgcgtgg acaggcggct ttcgcgccga
     1801 tcgaggtcat caccgacccg cactcgatcg tcaacagttc ctacgaggcg ccgaactcgc
     1861 agagcctgat gtcgatcttc accggcttca ccgccggtca gcacgcggtc gccaagttcc
     1921 tgtacgcctg ccccgagaaa tacacgaagg tccacgcacc gaccttcaac atgatcaaca
     1981 ccttcatctg gggcggggtc agccagcacg gcgagacgct cggcaacctg tgtgccgacc
     2041 tgaacggcat gggaggcggc gcacgctcgg acatggacgg agagcatgcg ctggctccga
     2101 tcttcgccac gatggccgac atcggcgagc aggagttgaa cgaagaagac gtgccgttcc
     2161 tgcagctggt atccaagaag atgaccatgg atgcggtcgc gccgggcaag taccgtggcg
     2221 gtcagggcta cacgatgatc gtcgccacca aggacagcga gcagtggggc ttcatgacca
     2281 cctgccaggg cgcaaagatc ccgccgatcc agggcctgtt cggcggctat gcctgcggta
     2341 cctatccgct gtcgaaggtc aagggcgtcg atgtctacga cgtgttgatg aacgagccgc
     2401 acaagttcaa gcactcgatc gccgagatca tgaacgagcg gccgttcgag gacgcgacct
     2461 acacgaccca tcacatgggc atgggcttcg agatctccaa gcgcggcgag ctgttcatga
     2521 tctcgcaggg cgcgggcgcg ggctatggcg acctgctcga gcgcgatccg gccgcggtga
     2581 tcaaggacat cgaggaaggc ctgatgtcac agggtgtggc ggcgcggctg tacaaggtca
     2641 agttcgatcc ggtcacgctg gccatcgacc aggcagccac cgatgcgctg cgcgccgacg
     2701 aacgcaaggc gcgcatcaag cgcggcgtgc cttacaagga cttcgtcaag acctggagca
     2761 agccgaagcc gccccggacg ttccagtatt tcggttgctg gggcgacgac atcggcacgc
     2821 tgtatgtcgg cagccctgac atcacccgcg atgccaatca gcccaagccc aactacatgc
     2881 gcaatccgaa ggacgtgcgc atcgatgagc tcgaagcgag actcgccgcg ttgggtgcgc
     2941 tgaaggaaga ccgccaatga gcgtggggtt tgccgagaag cttccggatg cggccggtca
     3001 aggccccatc cgggtctggc tgaagtcgtc tgcatacacc aggcgtgtcc ttctcggtgc
     3061 gtccacggat ccgtggacgt ccggcgcgca gtacctggcc tacttctcgc aggctcaggg
     3121 gctgctgaag ccggacgtcg cggtgctgga ggttggcgag ctctatgacg cctggaatgc
     3181 ctctcacggc gacctggctg gcgccatggg caaccgccgc aagcctgcca ccgcgctgcg
     3241 caagctgctc gaacccgaag acccgcgcaa ggtactggcc gaggtggtcg aggccgttct
     3301 tgcgcacctg cgcggccaga cgccgctggt tctctcggtg ccgtcgcccc gccgctggat
     3361 gaatcacgcg aaccgattga ccggtcgcga tgacatcgag gtgacgggcg acgatgtcga
     3421 ggatgcaacg atgtacatgg ccgacctgat ccggtccgtc tcggcgcacc cggtgtcggg
     3481 cctgttgctc gaggaaggtc gggacgacgt cgatttcaga gtcgaagact tgacgcgata
     3541 ccagtcgatc atcaacgtgg tgcggcacta ccgctggtcg gtggcgctgc gactgccagg
     3601 cggggctgct ccgagccccg aggctctggc ggatttcgac gcggtgatct cgccgacgct
     3661 cgagcaggcc ggtgcggctg cactcggtcg cgacatcagc gcatcgtttg cccaggacgg
     3721 agccattgcg ccgaggacgg gtcgacagtt ctatttcgcc gagatcgatc ccggcctgcg
     3781 ccccgagtcg gtgctcgagg cggtgactcg cttgcgaggt ggctgaatcc ccacgtgcgc
     3841 cggttgaccg gatcccggtc aacctgctca ccggattcct gggttccggc aagacgaccc
     3901 tgctcaacca ctgggtccag ctgccggcgc tcgccggttc tgccgtgctg gtcaacgagt
     3961 tcggccaggt cggaatcgac caccacctgg ttgagcgggt cgatgaacag atggtgctgc
     4021 tcgactcggg ctgcctgtgc tgcgcgatgc agggcgacct gatcggcgcc ctgaagaatc
     4081 tggctttgcg ggcctcgcgt cgcgagattc cgcctgtcac ccgggtcatc gtcgagacca
     4141 ccggcctggc cgatcccgtg ccggtgatct acacgttgat ggaagacccg ttcgtcgctg
     4201 cgcgctacgt ctgcgacggc gtggtgaccg tggtgtcggc aacgcacggg gcccagcaac
     4261 tgcgcgaatt ctccgaagcg acccgccagg tcgtcgcagc cgatctgctg ttcatcacca
     4321 agtgcgatcg ggccacgcca tcgatgctgg cctcacttca ggatgttctg cgagagctca
     4381 atccgggagc cagccagcgg caagttcgcg acggcaagga cgatgcgtcc gcgctcctcg
     4441 cttgcggcgt gtacagcacg gcagacaagc cttcgagcct cgccagatgg atgggcgaag
     4501 aagcgctgcg ccagggagaa ttgcgcgagc ggggcggagg tgggcgcgga tcggaggtgg
     4561 gcccagcttc cgacgacggt cgcttcgatg ccgagtcctc gcccgacgac gacgggatcg
     4621 gtcgtccgct cggctctcgc ctgcgcgagc agcgtgaagc ccgggacgcc gatcgccggc
     4681 gttcgtccgc atcacacacg gctggcgtgg cgagcttcgt catcacgttc gatcagccgg
     4741 ttccctggtt cggctttgcc gtggcgctgg gtcagatcct ctccagtcat ggatcggaac
     4801 tgctgcgtgt caagggcctg atcaacgtcg cgggcacggt gcgccctcag gtgatccagt
     4861 gcgttctggg agtggcgtat ccgtcggtga gcctgccggc gtggcccagg gaagggccat
     4921 tccgcgacgg acggggacgg ttggtattca tcacccgcgg gctcgacgaa gctcaggtcg
     4981 ctgcgatacg ttcggcactg gccacattgc cgggtgacgc cgttgcgttg cggatgagcg
     5041 ccggcgactc gatgctgccg acccggtgct ggctgtccca gcgcatgccg gtgacggcgc
     5101 ccgatgcgct cgagcacgaa gggtggttcg tgcaggccaa gcgcttcagg cgcggaggtg
     5161 gtccgcagtc acggtagggc gccgaagccc tgcgttttag ggctttgaat cgggcaaggc
     5221 gggcaagggg ctgggagcgg acaattccgg actggccttt tcgaaggtca gcaacttgct
     5281 gggctgcacc ctgacccgcg ccaggcattc gcccaccgcc tcggaaaact tctgcagatc
     5341 tcccatgtcg cagcggtctc caccacggac ctggatcctg gcgatctccg aacaggtgat
     5401 gccttcgaac tgcatgttgc ggcgaccttt gtcaccgggt cgcaccggac cgaacccggc
     5461 cgtcagggcg cggtactcga cgccattggc gcggaagacc gcgaactcgg gcacgaggtt
     5521 gcgaatcgcg taaggaagcg tgttcaccag gaccatctcg aatcggcagc cgccaggtgc
     5581 ctcgatgcga cgctccagca gcaagtcggc ctgcggttgt tccttcgcgg gctcggatgc
     5641 cgcgcccgcg ctggcttccg cggccttgtt cttgtactgc caggttccat cgtccttcag
     5701 caggatgcgc ttgcccttgc cgtcgtcgag ctcgaagtcg gcacgcgccg gcagcggcca
     5761 tgcagcggca agcacgagcg cacctgcggc tgttcccatc cagatcgtcg tcttcatgca
     5821 attcatgttt cagtccccgt ccaccggttc gagtttcggc tccagcccaa ccgccacacc
     5881 caatgccttg agcgagcgcg agtatcgcag tctgcccctg ctcgcggggc gggcgacgaa
     5941 agcacaatcc gaatcatgat ctgcgcagcc cgcatggcgg gaatttttac cactttcatt
     6001 ctccggacgc cggtcagccc cggttcgaac tgacatgccc gttcaatggt tcccgggtca
     6061 catgaactcg accaagaaag ccatcatcga gcgcgtcaag ggcatcgatg tggtgatcga
     6121 gatgctcgac gcccgcctgc cggcctcgag cggcaacccg cttcttgccg aactgacgcg
     6181 gggtaagccg gcgctgaagg tgctcaacaa gcaggatctg gccgatccgc agcgcacggc
     6241 gctgtggttg cagcatttca ctgagcagcc tgccacccgc gccgtcgcgc tggacggacg
     6301 cgctggcgca cccgtgcacc ggatcgtcga ggcttgtcgc gaactggcgc ccttgcgcgg
     6361 cggcatggtg aaaccgctgc gggtgctgat ctgcggcatt cccaacgtcg gcaagtcgac
     6421 ggtgatcaat tcgctggccg gtcgggtcgc ggccaagacc ggagacgagc cgggcatcac
     6481 caagctcgag cagaagatcg tcatcgccag cgacttctat ctgttcgaca cgcccgggat
     6541 gctgtggccg cggatcgtgg tcgagcaggg cggatacaac ctggccgcca gcggcgctgt
     6601 cgggcgcaac gcctacgacg acgaggaggt cgcgctggtg ctgctggcgg cgctccatcg
     6661 cgactatcca ggccaggtcg agcaacgcta caagctcgat gccagcgtgg cgcgcacgga
     6721 cgacgagcgg ctggctgccg tcggtcgcgc gcgcggcgct gtgctgccag gggggcgcat
     6781 cagcctgcag aaggccgcgg agatcgtgct gcacgacttc cgttcgggca gtctgggtcg
     6841 catctcgctc gagacgcccg aagactggct gcgctggcag gccgaagcgg cggtgaagga
     6901 ggccgagcgt gaggcctccc gtgcggccag atcgaagtcc aggcacgcct cccgatcgtc
     6961 gccggcggag ggttgaaggg cccgggggca ggatcaggcg ttgagcgcgc cgacggtgct
     7021 ttcgttcggc tggctgcgca agatgcggca gaggccgccg tacaccgtgt cggcgcacag
     7081 aggcttctcc cagtgctcgc gtgcgcccag gctcatggcc ttcgccagat cgatggagaa
     7141 ccgctccgcg gtcagcagga tcacgtgaca agcgctgtcg gtgatgcggt cgctgagcac
     7201 tcgcacgacg tccagtccac gcatgtccgg catgttcatg tccagcagga tcacatcggg
     7261 catggcctcc agagccgcgc ggatgccgtc gcggccgttg acggccagcc tcagcgtgac
     7321 tgccgggaat gctgacagca tcgcttcgac cagcatgcgg ttgaccgggt catcctcgat
     7381 gcacagcacg gtgccacgca ggtcttcgac gggtgcgtca ggttcgggct gggccatggt
     7441 cgtgtccctc ttcgaggtgc aacggtgggg agaactgcgc tgactgatcg tcaatgccgc
     7501 gccgaacgat gacattggta cttcccctcg ccagtaacaa ctgtcacccg aggcgacagg
     7561 gccgctggca gcccgccgta tgcttgcggc ccccacggag atcgagcatg acggaggttt
     7621 tttggaggct ggtgcgttgt tgcgtcgccg gcatgtcact gctgatcgcg atcgttcccc
     7681 agttcacgcg cgcacagtcg cccgtcccag tgtctgaaca tcaacatctc gaccggcacc
     7741 gcgagctcac gcggcagttc atgcaattgc tgaagtccga tccgcagcgt gcactcagcg
     7801 aagcgcttgc cggcgttcaa cgaaccagtt cactcggacg caacgaccca cagcgggccg
     7861 acgcactcga gatgctgagc catgcccacc tgatggcgga gcagtacgac aaggcactgc
     7921 cgctcgccgc agaggtggtg cgcatccgca aggccggccg tccggtcgac gacgaactgc
     7981 tcgcactggc actcgacacg cacgcgaccg tgctgtttgc caatgaacgc agtgccgagt
     8041 cggatgctgc gctgcgcgag tccctggcgg cctggcggcg ggcctacggt gcgaacgacc
     8101 tgcgtctggc cccgattctc gaaggccagg ccgagtacgt gcagaaggcc ttcggccgcc
     8161 ctctctggac gatcgaactg ctgcgcgagg ccgttgccat ccgcgtgcag aacgtcgggt
     8221 cgtctggcgg caagctggcc cagacgctga gcgaactggc gatccacgag atgcgtcagg
     8281 cgctgtactc cgactgcgac ggccacctgg cgaaggccca ggaactgctc gaggccgaga
     8341 tccgcagcga cccggcaagg caggagcaga agtccgggct catccaggtg ctcgtgatgc
     8401 gcgcgggcat cgctgccgtt ctggggcgcc aggatctggc attcgatcag gtgcagcggg
     8461 cgaggtcgat cgagttgacc gatcggctgc agcaggcgca agcgcaactc ctgatcgccg
     8521 agtcgttgag ttcggcgctg tggctgaccg gtgacctgga cggtgcgatc gctgccgagc
     8581 tggagtcgct ggacgttttc aagaaccacc aggacctgct ggaaagcggt gcgctcgaca
     8641 aggatctggt cggtgacacg ttgacctcgc tggcccgcct gtacctggag aaacacgaac
     8701 tcggcctcgc ccgacaagcc ctgaaggccg cccgttccgg cctgggcgat acgccggacg
     8761 tcctgttcaa cttgtccgag ctcgaacgtc agagcggcaa cgaggccctg gcgctcgcgc
     8821 attaccaggc tgcgctgcga ctgcgcaagg aaagcgccgc cgagatcaag gtgatgttcg
     8881 gcaccaaccg ggcagaggaa aggggcggcg agacggggcg cttcggcacc gcggtcggcg
     8941 accatctgtc gctgggcgag tcggctgtgc tggtgcccgg tgcgcagttc tccgatgtgg
     9001 cgtggctgcg tcccgcacgc accgtgccat tgccggtcgg tctggccacc gacgcgacgc
     9061 gactgctgct gcgcaacaag cgtgtgcttg atgccgccgc cttcggtgtc caggctcgcg
     9121 agttgatggc gcgtgcgcgg ctgtacccga aatcggcgct ggtgttcgtg catggattca
     9181 acgtctcgtt cgacgaggcg ctccagcgtg cagcgcagct cgcccgcgac ctgaactacg
     9241 acggcgcggt attcgtctac tcctggccgt cgcagggcac ggtgctgcgc tatggcaccg
     9301 accgcgacat cgccgaccgc gcggtggacc gattcgtcga gtttctcgcc caggtcgagc
     9361 tggctaccgg tgccgagaag atcaacctga tcgcccacag catgggcaac cgggtgctgc
     9421 tgccagcatt ggccaagatc gccgccgacc cgcagagccg ggtccgcccg aaggtgcgcg
     9481 aggtcatcct ggccgctcca gcggtgccgc aggccgactt cacgaactgg ctcgacacca
     9541 tctcgaagca gggcatccgc cgcttcacgc tgtacgcgtc gtcggccgac aaggcgatgt
     9601 gggcaggctg gctgcgcgaa tgggcgacgt cgcttgccgg atacgcctcg agcggcgtgc
     9661 cgctgtggca cccgagcgtg cagagcatcg acatctcgaa ggccgcggag ccgggcctgc
     9721 tccagctcaa ccacgacgta ttcgcgtcga accctgtcat gtccgaggac atccgccagt
     9781 tgctgcagca aggccagcag cggccgccga atcagcgcat gccgatgatg aaggtgcagc
     9841 gcagtccgga cgcgggacgc gcttactggt cgtacgagcc gcctgcacct gcgcggcgct
     9901 gaatgaatcg tgccgccgat tcctggctgt gccgaccggt caccggggct gccgctgcag
     9961 tcacaaagac gccgtggcca gcttcgcacc gaacccgatg aacagcgctc cgacggcgcc
    10021 tgtcagtccg gctgacagcc gccttcgccg gcggaactcg gccgcgaggc gcgcgccgac
    10081 gaggatcagc acggtgagat aggcgcagct ggtcagctcg cacaccaggc cgagcagacc
    10141 gaacgaccac cacggtgccg ggtaggtcgg atcgacgaac tggatgaaga aggacacgaa
    10201 gaacaggatc gccttcgggt tcagcaggct gatcaccagg gccctggcga agggatcgcc
    10261 cgtcaccggc tcaggcaggc gatcggtggc tgagtgccca cgctgctgcc acaggcgcca
    10321 cgcgccacgc agcatctgca agccgatcca gcccaggtag gccccgccga cccatttgac
    10381 taggccgaac agctccggac tcgctcgcag cagcgaggcg actccgccag cggacagcag
    10441 catcaggatc aggtcgccga ggaacacgcc gcaggcgccg cggtagccgg cccgcactcc
    10501 gcgctgagcg gcaacgctca gcacgtacac cgaattcggg cccggcagca gcacgatcag
    10561 cacggtaccg acgacgaagc tccagaagtc ggtgacgccg agttgaaggt ggatccagtc
    10621 gctcatcgct gaccctcgtc ggcggcgaca ggggccccgc cgcgtgccgt gcattgccgc
    10681 acccgaggtg accaccactt cggaagcagt cgccgcacct gcgcttgccg gtagcggtca
    10741 tcgatcagca ccaccacacc gcgatcattg ggcccacgga tgacccggcc tgcggcctga
    10801 accaccttct gcacgcccgg gtacagatag gcatagtcgt ggccacgccc ggcgccgaac
    10861 atctgctcca tgcgctccct gatctgttca ttgaccgggt tcagctgcgg cagccccagc
    10921 gtcgcgatga aggcgccgat cagccgtgca ccgggcaggt cgatgccctc ggcaaacgcg
    10981 ccgccgagca ccgcgaaacc gatgccctgg ctggtcggcg tgaagcctgc caggaagcgc
    11041 gcacgcgcgg actcgttcat cgcgcgcgat tgctcccaca gcgtgatctc ggggtgacgt
    11101 gtcctcagca gccccgacac gtcctgcagg tagtcgaagc tgctgaagaa cgcgaggtag
    11161 ttgcccggtg cctcggcaaa ctgcacggcc atcagttcga cgatgggcaa cagggaatcc
    11221 gcccgatgcc gaaagcgcgt ggacacggcg tcgatcacgt cgacgcgcaa ctggccggcg
    11281 tcgaacgggg atgccacatc gatgcacgcg gtgtcgtcgg gcagacccag caggtcgctg
    11341 acgtaatcca ccggattcag cgtcgcggaa aacagcgtgg tcgtgtggct cgctgcaaag
    11401 cgcggcttca ggaagggtgc cggcaccacg ttgcgcaggc acagcaccga gtcgtcgagc
    11461 ggctcgtcca gcgtcacgtc gaacagcgag tgatcgccga agtcctcatg cacccgcgtg
    11521 aaatgcagcg catcgaaata gaagcgcaac agcggtgagt cgagcgccgg ccgttcggcg
    11581 aggtgatctg tcacgaccga caccacctgc tgcagggccg cgatgaggtc ctgccgtggg
    11641 cccggataga cccggtagga cacgctctcc tcgccatcca cctgtgcgct ctcgatcgct
    11701 tgccagcaac ggtcgagacg ccgcagcgcg ggccgcacgt cggccggcgc cttcggcagc
    11761 agggcgcgca gatcggtgtg acgaagcgac gccgaataca tctgccgccc gcgctcgatc
    11821 aggttgtgcg cctcgtcgac cagcacgccg acccgccatt cgtgcgcctg cgtcaggccg
    11881 tgcagcagcg cgctcagatc gaaccagtgg ttgtagtcgc cgatcaccac gtcgctccag
    11941 cgcaccagtt cctgcgacag gtagtagggg cagacgtggt gggccagtgc gacttcgcgc
    12001 agcgtggtct ggtccagcac cgatcgcgtg atcgcatcgg ctcgggccga tggcagtcgg
    12061 tcgtagaagc cgcgtgccag cggacacgag tcgccgtggc aggccttgtc cgtgtgttcg
    12121 cacgacttgt cgcgcgccac cagttcgatc acgcgcagcg gcaacgtcgg ggcgctgtcc
    12181 cggatgacgc tcaacgcgtc cagcgcaagc gcacgccccg gcgtcttcgc agtcaggaag
    12241 aacaccttgt cgatctgctg gtccggacag gccttcagca gcgggaacag cgttccgatc
    12301 gtcttgccga tgccggtcgg agcctgcgcc atcaggcagc gcccggcggc cgcggtcctg
    12361 tagacggctt cggacaacgg gcgctgaccc ggccggaaat cggcgtgcgg gaaacgcaac
    12421 gcctccaggc ctgcatcgcg ctcgcgacga tgcccggctt cctgctctgc ccagtcgcga
    12481 aatcgccggc accgatcctc gaacaactgc tgcaaggact gcgcggagtg ccgctcggtc
    12541 agcacggtct cgtcctgggt cgcgacatcg aagtacacga gcgccagctc gacttcgtcc
    12601 agcccgcgct cggcgcacag cagcgcgccg tagacctgca cctgcgccca gtgcaatgcg
    12661 cggcggttgg ccggcatgga gtcgaggtcg ccgcggaacg tcttgacctc ttccacgcgc
    12721 tggcgccggg ggtcgtagcc gtcggcacgg ccgcgcacga tcaggccgtc gaactcgccg
    12781 cagacgccga cctcggcttc atacccatca ccctgttgcg ttcgccgcga tgagacgatg
    12841 gtgtgcccgg cgatgccttc cagcgcggtg gccgagggcg tgaagcgcag atcgaggtcg
    12901 ccggccttgt cggtgaactc gcacagcgcg cgggcggcga cgtggacgtt caagggagcc
    12961 cctcgttgcg ccaactcacg cgtcgggtgc cacgcccagc agtcgatgca gccgcgtgct
    13021 ggtcgtcgtg tactggacct gcaccgggcg gtcgggtcgc aggtacgcgg cggccccata
    13081 ggcagccatc gtcgcttcgt ggaatccgca gacgatgagc ttcttcttgc ctgggtaggt
    13141 gttgacgtca ccgaccgcga agatccgtgg gacgctggtc tcgaacttct cggtgtcgac
    13201 caccacctgc ttgcgatcga gctgaatgcc ccaatcggcg atggggccga gcttcgggct
    13261 caggccccac agcacgagca gcaggtcgac cggcaacgac agcgtggttc catcggcgcg
    13321 cgccacgacg agttgcgtca gcgtgtcgcc agcgctctcg cagccgatcg cctgggctgc
    13381 gacgaactgc atccggcccg cggcgcaggc tgcgcgcatc cgatcgacgg tcgcgggtga
    13441 tgccgtgaag gcgtcgcggc ggtgcatcag cgtgacgctg gccggacgat cgatgtccga
    13501 gtcggccagt gccagcgcgg tgtcaagggc agcctcgtcg tcgccgatca cgatgacccg
    13561 tcgccccttt gcagagggaa ccgcttcgcc ggaatggatc acctgccggc cgaagaagca
    13621 gtcgattccg tcgaccttga tccgacgggg cacgaaggaa cccacgccgc cggcgatgaa
    13681 gacggtgccc gcgcgaaaac gcacgcctgc ggtggtggtg acctcgaagc cgccgtcggc
    13741 ctgaggctcg accgcgctca cctgctgcga caggtggaat gtcggcttca tcggcgcgat
    13801 ctgcgccagc agtcgatcga cgagctcccg accggtgcac accggcacgg cgggaatgtc
    13861 gtagatcggt ttgtcggggt acagctcgat gcactggccg cccggcatgg ccaggctgtc
    13921 gacgacatgg caggcgatct caagcaaccc gagctggaag acctggaaca gacccgtcgg
    13981 gccggcgccg atcaccagcg cttcggtggt gatcggcgcg ccggggggcg cttgcaaggc
    14041 ggtgcggtcg ggcaaggcgc gaaccgcagt cgaggcagga agacgggcgt gccggaccgg
    14101 gctcagcgct tcagttcaga cagcttgccg gtcttgtctt tccactcatc ggcatcgggc
    14161 aaggccggct tgcgcttggt gatgctgggc cagccgctgc gcgccagatc ggcgttgagc
    14221 tggatcatgt gctgctggtc gccgggcacg tcctcctccg gcatgatcgc gttgaccggg
    14281 cactcgggga tgcagaccgc gcagtcgatg cattcatccg ggtcgatcgt caggaagttc
    14341 gggccttcgc ggaagcagtc gacggggcac acgtccacgc aatcggtgta cttgcagcgg
    14401 atgcaggatt cggtgacgac gtgggtcatg acagaggctc ggtgatgggt gtggaaacgg
    14461 caggatgagc agactggaaa ggccggtcgg gcgccggagc cggcgtgggc gaattttacg
    14521 tgctcaggtt ggccttcgag ggcgaaggga tgtggtcggt gtccggcggt tgcgctgcat
    14581 caatcggacg cgctccggcg cccaccagga cgagcgtcgg gcgacccgcg tgcagcatgc
    14641 tggcccgtcc gagggtctcg atcgtgtggt cgctgtgcag ttcatcgggc catccggcac
    14701 gcgaaatcac gcaggcctcg gtatctgttg gccagccggc acgcaccagc tggcgggcca
    14761 gcgcaccgag ctgctttcct gccatgtaga acacctcggt atcggcgctg cggctggctc
    14821 gaagggggcc ggaggccgcc tccgcggcca catgatcggg agccgcacgc gtcatcgcgg
    14881 tcgagaagct gacgcttcgg cccgagccac gtcgggtcag cgggcgcttc gccgaggcgg
    14941 cagcggccag ggccgctgtc acgccgggca ccacttcgca ctcgatgccg gcttcggcca
    15001 tggcctgcag ttcttcctcg agccggccga acacgctggg gtcgccgccc ttcagtcgca
    15061 cgacaagacc gcccgcaccg acctcacggg cgcttcggac cagcagcgcg ttgatggttt
    15121 cctgtccggt gctgtccgcg aaaccgcgct tgccgacgtg cagccagcga gcctgaggag
    15181 ccagttcgcg cagtgcggga tcggccagcg cgtcggtcag caccacgctg gcctgcgcca
    15241 gccgctgcgc tccgcgcacg gtgatcaggt cggcctgacc gggcccggcg ccgacgaagg
    15301 cgacgcggcc gaaggccgtc gtgcccgaag ccgctttcat gccgcggact tcaccagcag
    15361 cgcgccgctg gtgcggttgg tcgtcggatc gacgacgatc agcgagccgc cgctgcggtt
    15421 cgactcgtag gcctcgacgg gcagcggctg ctgcgtctcg atgatcacgt gcccgatctc
    15481 gttgacggcg agttcgtgcg catcgatcga ctccagcgag tggatgtcga gacggctctc
    15541 gatgctggcg atgcgtgcct gcacccagcg gttgccatgg cgcacccagt acttgcgtcc
    15601 gacgacggcc ggctcggtgt ccagccaggc cagcgtcgcc gcaaagctct gcaggccagt
    15661 gacggagtcg ggcgtcgaaa tccagtcgcc gcgcgagacg tcgagctggc gatcgagcac
    15721 gacaccggcc gagcggcccg cctcgacccg gtcgaccacg ctgccggcga gccgcacttc
    15781 ggccaccacc gcgcgctggc cgctcgggaa gatctgcacg tcgtcgccgg cctgcacgct
    15841 gccgtgggcg atccggcccc acagcgtgcg tggctggttg cccgtgcctt cgccttcgcg
    15901 agccacgtat tgcaccggga tcagcaggtc gccctcggtg cgctcctgcg cagcgggcag
    15961 cgattcgagc agctgcagca gcgtcgggcc gacgtaccag gtgctccagt cggtcccgtc
    16021 ggtcaccagc gtctgtgtga cattgtcgcc gcgcaaggcc gacaccggca cgatgccggc
    16081 gactgcgatg ccagccgcct ctgcgaacga gcgcagcgcg acgctgaccg cgtcgaacgc
    16141 ctgccgggga tcggcgaccg cgtcgagctt gttgaccgcg aacacgatgc tcggcacgcg
    16201 cagcaggtga gccagcagcg agtggcgacg cgtctgcggc agcagcacca aaggcgtctg
    16261 attcgtatcc agcttggtga tgtcgaccag caccaccgcg gcatcgctgc cggcggcggc
    16321 cgtcaccatg ttgcgggtgt actgctcgtg gcccggcgcg tcggcgatga tgaacttgcg
    16381 cgccttggtc gcgaagtagc ggtaggccac gtcgatcgtg atgccctgtt cacgctcggc
    16441 ttcgagcccg tcggtcagca gcgacaggtc gatgggcgcc ccggcagcgc gcttttccag
    16501 cgtgtcgagc tggtcggcca ggatggcgcg gctgtcgaac agcaggcggc cgatcagcgt
    16561 gctcttgccg tcgtccacgc tgccggcggt caggaagcgc agcgcccggt ggtcggtggc
    16621 gtccaggggc tcgtgggggt cggcccgaag gtcggcgggt tgcagcacgg cgctcatcag
    16681 aagtacccct ctttcttgcg gcgctccatg ctggcgtcgc tggtgcggtc gtccatgcgg
    16741 gtggcgccgc gctcgctcac cgtcacggtc agcgtctcgg ccacgatgtc ggcagcggtt
    16801 gcggcgccgc tctccaccgg gcaggtgcag gtcatgtcgc ccacggtgcg aaagcgcacg
    16861 gtggcggtct ccaccgtctc gccagcctcg ggcggcgtca cgtcggtcaa cggcaccagc
    16921 aggcccttgc ggcggatgac ttcccgctgg tgggcgtagt acaggccggg cagcgggatg
    16981 ttctcgcggg cgatgtacag ccacacgtcc agctcggtcc agttgctgat cgggaagctg
    17041 cggaagtgct cgccaggccg gtggcgggtg ttgaacagcg tccacagctc gggccgctgc
    17101 tccttgggct gccactggcc gaagctgtcg cggtggctga agatgcgctc cttggcgcgt
    17161 gccttctcct cgtcgcggcg ggcgccgccc atcagcacgt cgaagcggtg ttcctcgatc
    17221 gcctcgagca gcgccaccgt ctggtggccg ttgcgcgact cgagcgggtg cgacaggcgc
    17281 accgtgccgc gcttgatcga atcctcgagg tgcccgacga ccaggcgctc gcccatctcg
    17341 gccacgcggc ggtcgcggaa ctcgatcacc tcggggaagt tgtggccggt gtcgacgtgc
    17401 agcagcggga agggcaggcg gcccttgaat tcgttcgggt tgttcgggtc gaccttggtc
    17461 ttgaaggctt tctcggccag tcgcaacacc acgcaggaat ccttgccgcc ggagaacagg
    17521 agcgccgggc gctcgaaggc ggccacggtc tcgcgcagga tgaagatggc ttcttcctcg
    17581 agccagtcga ggtgtgtctg gtccagctcg gggagcaact gctccacgct gacggcggcg
    17641 ttcatggtct gtctccgatc atcgatacgg cgccctcggg cgcttcttcc ttgacgtgca
    17701 agccgcactc cttggccttc tcgtcctccc accaccagcg gccggcgcgg aaatcctcac
    17761 cgacggcgat cgcgcgggtg cacggcgcgc agccgatgct gggcatgaat tcatcgtgca
    17821 gcgggttgta gggcacgtca ttcgtggcaa cgtggtgcca gacgtcgttc caggtccagt
    17881 cggccagcgg gttgaacttg gtgcggccct ggccgtcgtc ctcgctgaac ggcaccaccg
    17941 cgcgcgtgtt cgattgctcg cggcgcagcc cggtgaccca ggcgctgcgg ccggccatca
    18001 tgcgcgccag cggttccatc ttgcgaatgc cgcagcaggc cttgcgcagt tccaggctct
    18061 ggtacatcgc atccttgccg ttcgttgcaa cgaaatgaac gaccgattcg ttcactggct
    18121 tgaacacctc gacggccaga ccgtagcgct gctcgatgcg cgggatcagc gccagtgtct
    18181 cggcgtgcag catgccggtg tccagcgtcg cgatcgcgat gccctgtgcg ccgcccagcc
    18241 cgtggcgcga gatcaggtcg gtcacgacca tgtcctcgac gcccaggctg gtcgattgca
    18301 cgatgcggcc gccatgcgcc gcagcggctt ccttcagcat cacgacggta tgggcgagtc
    18361 gctcctcgaa gccggctgtc ttgcgggcgt agaggtcgat cgcgctcata tggcagctcc
    18421 tccgtccgcg aactcagcag ccgattcggg cccggcgtcc gcacgcgcaa acctggggcg
    18481 acgctcccgg acatcgccct ggtagaagcc ggggaagaag gtcagcgcgc gttcagccgc
    18541 ttcgcgcgac tggtcggcgc gcagcaccac cgcatcgaag ccggtgcgat gcagcagcgg
    18601 cagcatgtcg accagcactt cgccaatcgc gcggatctcg cccgcgaagc cgtagcgcga
    18661 gcgcagcagg tgtgcctggc tgtaggcccg cccgtccacc catttcggaa actgcagcgc
    18721 gatcaggccg aagcgcggca ggtcggcttc gagttcgcgc acgttcaggt cgttgccgac
    18781 gatcacgccc accggcatgc cagctggcca gtggtggcac acgctgtgcc attgcagcca
    18841 ggtcagaagc aggtggggct tgggtgtgat ggtgaccatc gggccgtcgt cgccgtggac
    18901 ggtgtgccag gggtcctgtg cgtactggat gaatttcatg ggttacgtcc tcagtccgcg
    18961 cgcgcggcat cggtcgctga atcgcgggct gttgatctgc gcaccgcatt ggtcgcggcc
    19021 ttgaacgggt cgagcccgat gcgcctgacg gtgtcgatga atcgctcacc ggcctgacgc
    19081 tgcgcctgat aggtgtcgat gaccgcttcg accgcgtcgg ccgcttcatc ggcggcgaac
    19141 gaggggccga tcaccttgcc ggcgatcggg ccgccgctga tgaccgagcc gtcggagcca
    19201 ccgagcgtga tctggtacca ctcggcccca tccttgtcga cgccgaggat gccgatgtgg
    19261 ccgctgtggt ggtgaccgca ggagttgatg caaccactga tgtgcaggtc gatgtcgccg
    19321 atgtcccaga gctcgtcgag gtcctggtag cgctcggtga tctcggcagc cagcggaatc
    19381 gagcgcgcgt tggccagcgc gcagaaatcg ccgccgggac aggcgatcat gtcggtcagc
    19441 aggccgatgt tcggcgttgc gaagctggcg ctgcgcgcgg cttcccacag tgcgggcagg
    19501 tcggtttcga gcacccacgg cagcaccagg ttctggtcgt gcgagacgcg cagttcgccg
    19561 tggctgtagc ggtcggccag atcggcggcc agctccatct gcgcgtcggt gatgtcgccg
    19621 ggtgcctggc cggtgcgctt cagcgacagc gtgaccgccc ggtagccact gagcctgtgc
    19681 ctgtgcacgt tgcgctcgat ccagcgggtg tagccggcgg gcgcgcctgc ggcaaccggg
    19741 atgcctgtcg acggccgcgc cgcgactccc gcggggacca cgaagctcgc attgacacga
    19801 tcgaactccg actgcggaat gacgtgcgcg ctgccgtccg catcgcgttc gaggatgttg
    19861 cggaactcct cgttgacatc gtcgacgaag cgctggccct cggccttgac caggatcttg
    19921 atgcgcgcct tgtagatgtt gtcgcgccgg ccgtagcggt tgtagacgcg caccacggcc
    19981 tcgatgtaga cgaggatctg ctgccacggc aggaattccc ggaacaccgt gccgatcacc
    20041 ggcgtgcgcc ccatgccccc cccgaccagc accttgaagc cgacctcgcc ggccgcgttc
    20101 ttcaacaggt gcaggccgat gtcgtgccag ccgatcgcgg cgcggtcttc accggcaccc
    20161 gtgacggcga tcttgaactt gcgcggcagg aaggcgaact ccgggtgcag cgtgctccac
    20221 tgccgcatga tctcgccgta ggggcgcggg tcgaccgatt catcgggtgc gatcccggcg
    20281 aagcagtcgc tggtgatgtt gcggatgcag ttgccgctgg tctggatgcc gtgcatgttc
    20341 accgcagcca gctgatccat cacctcggcg ctcttgtcca gtgggatcca gttgtattgc
    20401 aggttctggc gcgtcgtgaa gtgcgcgtag ccgcggtcgt agtcgcgggc gatgcgagcc
    20461 aggccacgca attgcgccga gctcagttcg ccgtagggca cggccacgcg cagcattggc
    20521 gcgtggcgct gcacgtacca gccgttctgc agccgaaggg cccggaactc atcatccggc
    20581 agccgcccgc cgaggtggcg atcgagctgg tcacggtatt gcgcggctcg ctgccgcacg
    20641 aattgcttgt cgaattcggt gtaggtgtac atcttgaatt agcgcgtcgg ttcgatgacc
    20701 agtgattaat gaatgacctt gatcccggcg gtcaccagcg acacgcagag cagcgtgcgc
    20761 accagccgct cgggcatctt gcgggtcagc tgggcgccga gccagattcc gggaatcgag
    20821 ccgaccagca gggcaacgag cagcgaccag tcgacgttgc cgagcgtggc gtggccgatg
    20881 cccgccacca gggtcagcgg cacggcgtgt gcgatgtcgg tgccgacgat ccggtgcgag
    20941 gccagtttcg ggtacagcag caggatcagc gtcgcgccga tcgcaccggc gccgatcgag
    21001 ctgagcgaca ccagcacgcc gaggatgaat ccgcaaagca ccgtgagggc cggtcgattg
    21061 ccgtcgttga tccagcgttc gagccaatgc ccgaccttca cccagatctg cttgaacgcc
    21121 acggtgaccg ccgtcagcag caaagcgatg ccgagcgaga acgtcagcgc gctggcccac
    21181 cctttggtca gcccggtgaa atgcatcagc gcgatggtcg ccagcgcggc cgggatgctg
    21241 cccgccagca gcaagcgggt gatgcgccac tcgacgtgcc cggcctggtg atgcgacgcc
    21301 gatccagcga tcttcgtgat cgcggcgaac cacaggtcgg tgccgatggc cactgcggga
    21361 ctcaacttga acaccgacag cagcaacggc gtcatcagcg agcccccgcc cacgcccgtc
    21421 atgccgacga tggcaccgac gccgaagcca ctgaatatcg cgattgggtc catcaagagt
    21481 ccggaaagcg gtccgtggcg acgtgcccgg ccgaaagcag cccgcgactt taacgaggac
    21541 ccttagcttg aagaactata tttttggacg accaccatgc tgaatgcata taaggaaatg
    21601 cgattcaagg tcggtctgat cccacccgac cgtcatgcga cgggtcaggt ggggagtcca
    21661 ctccacctta cttggcgggc cgaacctgtt cggcgacagc cggcacgcgg cgggcgagtg
    21721 cctggaaatc gatggcctgc atcgtgatgg catcggcggg gcagcggccc ggggcgtctt
    21781 cgcccacggt ccagtccttg gcttcctcgc tcaggctggc ggggcgagcg atgcccagtt
    21841 gcgccgtcag ctggctcatg ccggcgtgcg tgcgtgcgaa ggccagcgcc cggtcgtact
    21901 cggcattggc gtaggcacgg cgcgcggtgt agagctcgtt ctccgagttc agcagatcga
    21961 gcaggctgcg ctggccgatg tcgaactgct ggcgataggc gtcgcgggcc ttttcgatcg
    22021 ccagcgtgtt gcggtcgaga agcaccagct gctcggtcag cttgcgggtg tcgttgaacg
    22081 cgatcgcggc ggtctggcgc acgtctcggc acgccttgtc gcgcaggtct tcagcctggc
    22141 cgaccagatt gacctgctgg cgcacgcggg cctgatcgct gccaccgctg tacaggttcc
    22201 agttcagcac cagctcggcg gaactgtcgt gcttctggtc tcgcacacct tcgtagttgt
    22261 tgccgaagcc gctgcgcacc cgggcctcga tccgcggctg gaacgcggac tcgcgcacct
    22321 gtgccgaggt gcgggccgcc cgcactgcct cgacgctggc gctgatggcc gggctctgca
    22381 gcacggcgcg attaagcact tcggtgctgc tggcgggcag gtcggtggtg agcacggtcg
    22441 gggccccgcc cgccggcgcg gccttcgggg gcgcctcgcc gatcacgcgc aggtagcgcg
    22501 ccatcacgtc gtgcaggttg gcgacctcgg tggtcaggtt cgactccgcc agggccagtc
    22561 gtgcgttggc ttgttcaagg tcgacgcccc gacccacgcc cgcgcgaaag cgcgactgga
    22621 tctgctgcga tgcgtagcga tgctgcacgt agttgtcctc tgccagcgcg acgagacgct
    22681 ggaagcgcag cacgtcgtag tgggcgcggg tggcttcgag cgtggtctgc tcggtggcct
    22741 ccagcaggtc gaagtagcgg ctgagccggt cgtggctgac gcgatcgatc tcgcgctgcg
    22801 tgcccagccc gtcccacagc agttgcgtga ggctcaagcc caggccggtg cggcgcagcg
    22861 attcggattc gggctggcgc gaggtgatct tgtccttgtc gatgccgacg ctggcgttca
    22921 ggtccacacg cggcctcagg gctccacggg cgacgtcgac ggcgtcgccc gctgcccggt
    22981 aggcattgaa acgcgaggcc acttcagggt tcgacagcag ggccttctcg accgcgctgc
    23041 gcagcgggtc ggatgcagct cccgagcttt gggcctgcac ctgacaggta ttgctcaaca
    23101 aggcgatggc agtgacgatc agcgtgcgtg ataggcgcat ggtttctggt tgctagatgg
    23161 agggcggggg ccgtggacgc gcgacatgtc cagcggcgtc aagagaggca ggtgcccgag
    23221 cgaacggagt ctatgcgttg atatctgcgc ttcaggcgcg tgcttgacgt atttgtaaca
    23281 tctggtctcg gcctgcgtgc agcacgtcac ggcacgcggc tgcctggccg atcgtcgtcg
    23341 gcgcagccct tcccattcaa gttgccacgg tgcgtgacga caacgatggg tgctcctgcg
    23401 attgcccgct acgcgaattc cacccttcat gccgaccgtt cctcgacgct cgcgatacgc
    23461 ggctgcgtgc tgggtgctgc tgggctgtct gttgtcgttc gcatcggtcc cgtgggcgtg
    23521 ggatgccgag cgtttgtccc ttgccgcggc ggggcatggc gcgagggcct cggccgcgct
    23581 gcgcgcgttg cagcccttgc tggcggcttc gcccgatccg gacgaacgcc atcgcctgca
    23641 ggcaatcaac gagttcttca accgccgagt ccagtttcga gatgatctcg aggtgtgggg
    23701 acaggtcgac ttctgggcca gtccgttcga gatgctggag aagggggcag gggactgcga
    23761 ggacttcgcg gtggcgaagt acttcagcct gatctctgcc ggcatggcgg cgcagaagct
    23821 gcgtctcgtc tacgtgcgtg cacagatggg ggggcccggc ggggcaattc aggcgcacat
    23881 ggtgctggcc tactacgcca cgccgaacgc cgaaccgctg attctcgaca acctgatcac
    23941 cgaggttcgg cctgcgtcgc gccgacccga cctgacaccg gttttcagtt tcaatgccga
    24001 cgggctctgg cagggtgtcg gagcgcaggc ggccggcgat ccggtggcgc ggctgtcgcg
    24061 ctggcgggat gtgatggcca aggcccgcgc cgaaggattt gagccgtgac gccgaagcgc
    24121 caccacatcg attacaagga gcgaacatgt cgctgattcg tcaagtctgg ctgctgctgc
    24181 tggccacggt actgctggcc ttcgccggca gcgtgggagt ggtcaccacg tcgacgcgtg
    24241 atcttcttca gacgcagctg cggctcaaga acagcgacaa cgccgccacg ctggctctgg
    24301 cgctttccca gcagaagggt gatgcggagc tgatgtcgct gctgatgacc gcgcagttcg
    24361 acaccggtta ctaccggcag gtgcgcctgc tgtcgcccga gggcaaggtg gtgttcagcc
    24421 gcgatgctgc ctcgaaagcg gcgcaggctc cggcgtggtt tgtttccgcg gtgcctatcg
    24481 agtcacagcc gggtgtggcg caggtcagcg atggctggcg tgcgctcggt caggtgcagg
    24541 ttgaaagtca ggtgtcttac gcgcacgacg cgctctggac cagcaccttg cgttcggcgc
    24601 tcgccatggc cctcgtgggc ttgctggccg cggcgctcgg catgtgggtg gtgcgtcgca
    24661 tcagccgccc gctcgatgcc gccgtggccc aggccaatgc gctggaacac ggcgagttcc
    24721 tgaccgtgcc cgaaccacgc atgcccgagg tccgccggct cacccgggcg atgaattccc
    24781 tggtcgcccg acttcgcctg tcatcgggtg cgcaggccga gcagattgcc gcgctgcacc
    24841 ggcaggccac cggcgatgcg ctgacgggcc tctccaaccg cacccacttc ctggtccagc
    24901 tcaccgccgc cttgcagcgg gaggacggtc cggcggacag cgggctggtg ctgctgcgcg
    24961 tgatggacct gggtacgctg aaccggcagt tcggccatgc cgtcggggat cgcatactgg
    25021 taaccatcgc gcagtcgctg aagccctatg ccgagggtgt caagggctgc ttcctggggc
    25081 gtttgaacgg gtccgatttc gcgttgtgcc tgcctgcccc cggggtggca ctcgagaccg
    25141 cgcaggcggt ggcggaagcc ctgcggctcg tgctgccaac cctcggcagc ggcgcggcgg
    25201 tgtgcctggg ggcggtcgaa atgcaccgtg acctgtcacc cggccggctg atgagcgcgg
    25261 cggacgaggc gctggcgcgc gccgagttgc gcggcgcgtt cgcggtggag ctgggcagtg
    25321 aaccgcccgg agcgattgca ttcctcggcg agggtgcgtg gcgccagcgc atcgtgcagg
    25381 cgctagacca gcagcgggtg cgcctgatgg agtttcccgt tctcaattcc cggcgacagc
    25441 tggtgcatct cgaatgtccg ctgcgcttgc agctcgagag cgggggagct ttcgagccgg
    25501 cggcacgctg gcttcccctg gctgctcgca gccgtctcac cgtcgatata gacgagcgcg
    25561 cgctgacgct ggcgctgcag gcggcactgc atgacggaca gccaagatgc gtcaacctgt
    25621 cgccggcctc cctcggcgac agcggattcg ctgcacgcct gcgtgcacgc ctgtgggccg
    25681 tgcctcaggc cgccaaactc gtctggctcg agatcgatga aagcgctgcg gtcgagcagt
    25741 tcgacctgct gcgcgagctg gcgcggcaac tccggtccac cggggtgcgt ttcggcatcc
    25801 agcatgccgg cgaacggctg ggccagattc ctcgcttgtt cgaggccggt ctcgacttcg
    25861 tcaagctcga tgcgtcggcg acgatcggtg tcggacgcga cgatcaccgt gcgcttttcg
    25921 tgcgcggcac cgtcacgttg ctgcacggca tggcgatcca ggtgtacgcc gagggtgtca
    25981 gtgattcccg cgacgcctcg cgcctgtggg actgcggtat cgacggcgcc accggcccct
    26041 ggatcagcac gctggacgtc gccgcgggct gaggccagcg gcctcgaccc ggccttgctg
    26101 gctcaatcgg tgacgagttt gccgcgctgc agcaggtcgt tgatgacctg atggtcgttg
    26161 ctgaatccgc cgacgaggtt catgccgctc agcgtgatca cctgatccac cgctgccggg
    26221 ttgtaggtgc cacccgcgaa gccgccggtg gaactgatct cgacgacggt gtcacccccg
    26281 ctcaccgaga agtgcaggaa gttggccagg ttgccttcgt tcggggtcac cgtgatcgcg
    26341 ttgttcagcc cgatgctggc cggaagcgcc acaccgtttg ccgcgtgcga ctcgccgacc
    26401 agcaggtcac gcaggtcgag cacgtctccg gcgtggttcc tgttgtcgaa gtcggcaatg
    26461 acatcgtgcg cgggtgtcgt gacggaacca ccatcgccca gggaccagcg gaagacgtca
    26521 tcgccggcac cgccggtcat ctggtcgtcg ccctggccgc caatgatcag atcggcgccg
    26581 ccgcctccgt tcagcgtgtc gtcgcccagc ccgccgatca ggcgatcgcg gccggcgttg
    26641 ccggtcaggt cgtcattccc gccgccaccg tcgaggactg aaccggtgcg gatgttgtag
    26701 gtgccggggg ctgcgccggc aacgatgtcc tgcaggtccg tcagagcggg ggcatgtgca
    26761 tcgctgaaca gtggcacgtt ctgagagccc aggatcgcgt agccgctgtc gggactgccg
    26821 ctcggcttga actcgacgcg cagcaccgcg ttgatgcctt gttcccagta gatcagctcg
    26881 agcggcgtca tgcctccgtc gatcggcacg ccggtataga cgcgcgtcgt cggcgactgg
    26941 atgccgtcga agatggccac atcatggttg cccagccgca gccggaagcc gtcgtctgca
    27001 gtgacgcgaa tgtcgtacat gccggctgcc aggtaggcct ggccggtgag ccggatcgcc
    27061 gcatcgctgg tcttgcccag gccactggtc ggaccggttc cactggtgtc gctgtcgccg
    27121 gtgccctggg tgatccgcag gctgccggcg tcgctgccta ctgcgcggtt cagcaggcgg
    27181 aacagctggc tgttgtcgtt gttcagcgcg gcgattgacg cgcccgctgc caggttggga
    27241 ttcgtgccga gcgtgttggt gaccgtctgc gtcacgccgt agtcgatcgc cgtcgaggtg
    27301 aatctcgcgt ccgtcgcgtt ctcgttggat gcggtcgtgg tgccgacgac gttgccgcca
    27361 ccgaaggctg cgttgcgtcc attgacgatc gcattgatgt ccgacacgtg atccaggttg
    27421 cccagggtgc gatcgtcacc gtggcgccga ttcgagttgg tgccgttcgg gttgaagtcg
    27481 ttgtagccgt agtactcgcc gctcaggcca agggggttgg ccagcggatt gatggtggga
    27541 ggatctgccg tcaccgtgtc gccttctcct gcgacgtgca gcgtcaacgt ggccgtggag
    27601 agacctccgt tgccatcggc tacctcgtag gtgaacacat cgtctgcctg tgagccggtg
    27661 gcgatcgaac ctgcccgatt ggcgatgtag tcgtagctgc cgtcgctgcg cagcagcaga
    27721 tcgccataga tcccggccac cgtgacgccc gccggcgaga cggctgtgct cggtgcgccc
    27781 gtaccggcga cgacctgcat cacggtcagc gaatcacctt cgggatcgga atcgcgcccg
    27841 gcatcgaccg aagtcgacag gatgatgccc tctgcggcgt cgacggtccg ggtctgcgat
    27901 gcctggacgg tgccttcatc gttctgtgca agtggcgggt cgttgatggg tacgacgtca
    27961 agcgtgaaag tggcaggagc cgatgtcagg ttgtcgttgt cggtgaccgt gaagaccact
    28021 tcggcggcgc cattgaagtc agggttcggc ttgaacacca gctgtgctgc gtcagctggc
    28081 gacagcggtg tgcccacggt caccggtgtg gtgccgtcat gcaggaacag cgtgccgttg
    28141 cttggcagtg tggtgacggt caccgactgg actgtgccgt cggcgtcggt cccggtgagc
    28201 gataccggaa gcagcgtgtc ctcgtcaccg gaaaccgagc cgggggtggc atcgggggcc
    28261 aggttcgggg ccggcgagac ggtgatgttg accgtggcgg tgtcggtgcc gccctggcca
    28321 tcggagacgg tgtacgtgaa agaagccggg cccgagtagc ccgcggtggg ggtgaacacc
    28381 acattgccgg cgacgatcga gaccgagccg tgcagcgcgt cctgcacgct ggtgatcgcc
    28441 agcgggttgt tctcgggatc ggtgtcgttg cccagcaagt ctgccggcac gaacgtcagc
    28501 ggcgtgttga aggcggtcga gcccgagtca tcgatcgcat cgggggccag gttcggggcc
    28561 ggcgagacgg tgatgttgac cgtggcggtg tcggtgccgc cctggccatc ggagannnnn
    28621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaacg tcagcggcgt gttgaaggcg
    28741 gtcgagcccg agtcatcgat cgcatcggga ccggtcggcg ggggcggcgc cgcagcgacg
    28801 ttgatcgtca cggttgcggt gtccgtgcca ccttgaccgt cactgatggc gtagttgatc
    28861 agggccgtgc ccgagaagcc cgatgccggc gtgaacagca gcgtgccatt cggggcgacg
    28921 gtgacgcttc cctgcgcggg attgaccatc gtggcgctgg tcaccgtcag tgggtcgccc
    28981 tcggggtcgg tgtcattggc cagcacggag acgctgacac ttgtgttgtg tgccgtggag
    29041 gcggagtcgt tgacagcatc cggcccatcg ttgaccggcg tgatgatcaa cgggacggtg
    29101 gtcggcgccg agggcagccc ctcgttgtcg gtcacggtga aggtcagatt caccgtgccg
    29161 aagaagttgg gtgctggcac gaacaccagg ttcgctgcct cggtcggggt cagcggaact
    29221 cctgcgatca ctggtgtggt gccgtcggaa aggaacagtg ttccgttggt cggcagtcgg
    29281 ctgatggtga acgacgtgac cgtgccgtcg acgtcgctcg cagacaagga aaccggaagt
    29341 ggcgtgtcct ccggaccgat ggccgagaac ggcgcagcca cgggcgcgtc gttgacgctg
    29401 atcacgttga tcgtcacggt ggcaggattc gacgccaggc cctcgttgtc ggtcacgaag
    29461 aaggtgatcg cggtctggcc attgaagttg gccgatggct gaagcagcag gcctgccgcc
    29521 tgtcctggtg tcagcgtctg tccggccatc acgacagtcg tgccgtcggc gagcagcaag
    29581 gtgctgccgg ggggaatgga ggtcacggtg acgctggcca cactgccgtc gccgtcgttg
    29641 ccgatcagcg gaacgggcag ggtggtgtcc tcgttgccgc tcactgcatc gtccagagcc
    29701 tctggcgcga tgttgttgac ggtggctccg gtaccggctt ctgcagtgat gccggctgcc
    29761 gagttgctgc tgaaagccag cccagccggg gtgattccct cctggatgcg ttcgacgcga
    29821 agaccgggga ggaactcgcc gccatctccg ccgttgagtc cggcgcctgg cgccgcatcc
    29881 gggtcgccct gattcaagtc ggagatgacc cggtcgagtt cgggagcggg tgctgccttg
    29941 atgggggcgg cggtcacttg cgcctcatcc tgggtcagtt ggacgatgcc gtcctgggtc
    30001 gtcaggatca cgtcgccgcg ttggaccaga tcccccatct tcagcgcacg catccggcca
    30061 tcgggcccct tgatcagggc gctgccccac agtccggtga ctcgtccctg agcggtttgc
    30121 aggatggtgg gcatggcggt ttctcttcaa ggtgcgcacc ttcatggtgc gatttcaggg
    30181 cgtgccgagg gcggacgata ggcaacgagc atagccattc cgggtctgca ctaagcggct
    30241 gcgaagggct tcatttcgtg attgaggtca ccagaaatga cgaattcgga acaataagga
    30301 agtgatcgcc tgggctgtca gggccgccgt gaccggcaac ggtcggcggg cgatctccgt
    30361 ggctcagcgc tcccgcagtg cgtaggcctt ggccttcaga accggcttca gcagatacga
    30421 catgaccgtc tttcggccag tcaggatgtc gacctccgcc gtcatgccgg ggatcaccgg
    30481 aagcttctcg ctgaagttgg cctgcgtggt gcgcacccgc acgaggtaga aggcattgcc
    30541 cttttcatcg acgacggtgt ccgggctgat gttctcgacc ttggcgacca ggccgccgta
    30601 gatcgagaag tcgtaggcgg tgaacttgac cgtggcgtcc tgccccgggt gaatgaacgc
    30661 gatgtcgcgc ggctggacct tggcctcgag aatgagctgg tcgtcgagcg gtaccacctc
    30721 gacgatgtcc ttgccgggct gcaccacgcc gccgaccgta ttggccagca gccgctggat
    30781 ccggccgcgc acggggctct tgacctgcga cttgtcgacc ttgtccgcca gcgcaaccgc
    30841 gccttcgttc agcgcattga gcttgcccat cacgtcggcc atctccttgc gcgcctcgtt
    30901 gcggaaggtg agttcggttt cctggatctt gcgttgcgcc tcgccgatgg cagcctgcac
    30961 acgggcgatc tgcgcggtgg cctgttcgat ctcgccgcgg ctgcgcgaga cctcccgatc
    31021 gagccgcagg atgtcgacct ccgacaccgc gcccgtcgcg agcagcggcc tggtcttgtt
    31081 gagctcctgc tgaccgaggt cgagtcctcg ctcggccgac gacttgcggg ccttcatttc
    31141 gctcagctcc tgctggcgtt gctgcaactg ctgctggttg atggcgatca tcgtgcccag
    31201 ctcggagcgc cgggtctcgt agagcgtgcg ctcctcgtcg aggatgcgac cttcgatcgc
    31261 gttggtgctg gtcggcggct tgaaggcgga gccttcggcc agggcgcgca agcgcgcctg
    31321 gcgtgcccgc agcgcgaaac cctgggccgc gctttcgcgc acgccggagg tggcccgcgt
    31381 ctcgtcgatc ttcagcagca actggcctgc ctcgaccacc tggccctcct ggaccaggat
    31441 ctcggaaacc acgccgccgt ccagcgactg gacgatctgc aactgcttcg acgggatcac
    31501 cttcccatcc ccgcgtgtca cctcctcgac ctgggcgaag caggcccaca gcaccagcag
    31561 gagcagcacg accacagcgg ccctcacgat gagctgggcg cgctgggtgc gggcacgcga
    31621 catcacgacg tccgcctgct gctcgaagct gccgaaaccg gctgcatcgg cagcggcatc
    31681 tgtcgcgccc cagcgggctg aagcaacctt cgcgatcgcc ttcatgcggc cctcgcgatc
    31741 cggccggtgg acagcgcttc catgatgcgg tcgcgcggtc cgtcggcgac gaccttgccg
    31801 ccgtcgatga cgatcacccg ggtcgccatg gccagcatcg aggtgcggtg cgtcaccagc
    31861 accaccgtct tgtcactggc gaaggcggtg accttggccg tgatctgggc ttcggtcgag
    31921 aaatccatcg cactggtcgg ctcgtcgagc aacagcagcg gagcgttgtg cagcaccgcg
    31981 cgggccagcc cgactccctg ccgctgtcct ccagacaggg attcgccgcg ttcgcccacc
    32041 ggcatgtcga agccgcgcgg gtggcggttg atgaattcgg tcagcccggc cacttctgcc
    32101 gccgcaacca cggccgagtc gtcggcatag ggcaggccga acgtgatgtt ctcgcgcagc
    32161 gtgccgtgga acagcgtcac gtcctgggac acgctgccca ggttgcgccg cacgtcggcc
    32221 ggatcgagct ggcgcaggtc gatgccatcg agcagcacgg cgccatcggt gggctggtac
    32281 aggcccatga tgagccgctg gatcgtgctc ttgcccgagc cgacgcgacc gatcagcgcg
    32341 acgcgttcgc cggcggcgat cctgaagctg atgccctcga tggccgaatc ggttcggccg
    32401 gggtaggcga acctgacgtt gcggaactcg atgtcacctc gcagctgggg ccgctggatg
    32461 aagttctcgt ttgccggccg ctccaccggc ttcgccatga tcttgttcag cgagtcgagc
    32521 gccgtgcgcg cgccctggta ctgcatcagc agaccgacga tctgtccggc aggcgccagc
    32581 gcacggcccg agagcatgct ggcggcgatc agcgcgccca tcgtcagctc gcgctggctg
    32641 atcaggtaga cgccgatcac gatcatggag accgccacga gctgggtcag cgtgctggtc
    32701 gcgtacatcg cacccgaaga caggccgcgc atcctgacgt tggtgccggc gagaaacagg
    32761 ttggcccgct cccacttcga ctggatgacg ctttcggcgc cctgcgactt gatggtctcg
    32821 atggccgaca ggctctcgat cagtgtcgcg ttgcgctgcg cgctggcctg gtaggtggtc
    32881 tcggacaagg catgcagccg gtgctggagg aaatagccga gcacgaggat gatcgagaag
    32941 gccaccagca ccggaaccgc cagccacggc gagatccacg ccagcacgac gacgaacagc
    33001 agggcgaacg gcaggtcgat cagtgcggtg acggtgctcg acgcgatgaa gtcgcgcacc
    33061 tgctcgaatc cgcgcaggtt ctgcgcgaac gagcccaccg attcgggccg gtgttccagt
    33121 cgcagtccca gcaccttctc catcagcttg gccgacagcg ccacatcgat gcgcgcgctg
    33181 gcctcgtcga cgaaatggct gcgcagcagg cgcatgaaca gatcggctcc gagcaccagc
    33241 agcacgccga tcgacagcga ccacagtgtc tcgaaggcgt tgttgggcac cacgcggtca
    33301 tagatgttca tcgagaacat cgggaacacg agggcgaaca ggttgatcag cagggccgcc
    33361 cacagcacgt cgcggtagac gaaccgctgc gccagcagag cactccagaa ccagtgctgc
    33421 tccgtggccg tgcgctcggg cggcgtctgc gcgccggcat cgaatcggaa atgaggccgt
    33481 acgaacagca cgacaccgct gtagcgcgcg gccaactccg tgcgcgccag gcgcacggcg
    33541 ccctgacccg tttcgggcag cagcacacgg gcttcgtcgc catcccagcc gagcagcacg
    33601 caggcctggt tctcatgcag gatcagcagc accggcaagg tgaccgagtc gatgccgtca
    33661 agcgcctggc gctgcagtcg ggtcgccatg cccgcgcggg ccgctgctcg ctcgaccaga
    33721 tcgaggggca ggcgaccctg cgtgtctgcg ccgatcggca accccgccga cagggcggct
    33781 cgcgtggtcg gatgaccttg cagcctgcag atctcgacca ggcagtcgag cagggcgtcg
    33841 gggtggatca ggtcttcgcg caggcgctgc atggtcccgc cggcgggagc ggcagcaggt
    33901 ccgggcgggg cttcagccgg gtgggccggc ggcagggagg tacgggagtc aggcatgccg
    33961 gggaacgatc gttcacccga tatcggcaca gtggaagccg aattgacccc ggcgatggtc
    34021 ttcgaagtac aacttcaggg tctcgcgcac ggtccggaag gcgaggtctt cccacggaat
    34081 ctcgtgctcg tggaacagct gcgcctcgat ggtctccggc ccgggtgcga agcgggtgtc
    34141 gagcaggcgg gcccggtagt acaggtgcac ctgcccggcg cgcaccacgt tgagcaggct
    34201 gaacagcggt tgcagttcga tgttcgcgcc tgcttcctcg acggtctcgc gaacagcgcc
    34261 ttcggccagc gtttcgttca gttccatgaa gcctgcaggc agggtccagt aaccgtagcg
    34321 tggctcgatg ttgcgccggc acaggagcac ctgatctccc caggcaggca cggtgccgac
    34381 gatgttgcac gggttctcgt agtgaaccgt ggcgcacacc gtgcagaccg cgcgctcccg
    34441 gttgtcgtcg gcgggaacgc ggtattcggc ggcactgccg caggtcttgc agtgccggat
    34501 gctgcgtgtc ggatgcatgc gtctcagtgt agcggcgcgc tccccgggcg cccaaggcgg
    34561 gcacccttgc gtggcatcat catcggccgc atccgtgcac cttactgaca tctcctgcat
    34621 ggcgtttcat cccgactggc tgcggccccc ctggaagaca cccggcgtga gtgccgtcat
    34681 gacgacccgt cggggcggtg tcagcgcggg accctacgcg agcatgaacg tgcgggatgg
    34741 ggtgggagac gagcctgaat gcacggcgcg caaccgtgcc ctgctgaccg aggcgatcgg
    34801 cgcagcgccc acctgtctcg atcaggtgca cgggctgggc gtcgcgcgac tgaccgcacg
    34861 cgattccgcc ccgcatgcgc cccgtcacga ggccgacgcc tgtatttcca ccgagccagg
    34921 cgtcgtttgt gccgtgcagg tggcggactg cctgccggtg ctgttcgccg ccccgggggt
    34981 ggtcgcggcg gctcatgcag gttggcgggg tctggctgcg ggtgtgctcg aggccacgct
    35041 ggatgccttg tgtgtcgctg gtggctgcgc gccagaccag gttgaagcgt ggctcgggcc
    35101 gtgcatcggg ccggactgct tcgaggtggg tgccgatgtg ctgtcggcgt tcggcgtcga
    35161 cccttctgcg cctgaagacg tgccgggccg gggtcgggcc gctgcgcgtt tcagggccgt
    35221 gaatcagggc aaatggcttg ccgatctgcc cgccctggcg cgggatcgcc tgacgtcggc
    35281 cggggtgggc tcgatcagcg gcggttcaag gtgcaccgtc tccgatccct cacggttctt
    35341 ttcctaccgt cgggacggtg tcaccggccg gatggttgcc gccatctggc tcacgggttg
    35401 acgcttcgtt ggtcgaaccg agggtcgccg aggcctctgc aagctgccgg gcgcgccggc
    35461 gggcgggtgt tcgcaggatg tacaggacga tcgccagcgg gaacgcgcca tacagcagga
    35521 acgtggaaac ggcgccgatg atggtgccgt tcgggctggt ggcctcgacg acggtcatca
    35581 tcaccaccac gtacatccaa gctatcgcca ccaggtacat caggctgtcc gttcaggtcg
    35641 agggcgtgaa tgggtattct cgcgggttgg ctgtcatgct gaggtcagca tgtcagcgta
    35701 caaccttggc gtggccccgc tgcgcggtcc gccggattca gtgaaagaag agacgagtga
    35761 aaaccaagcc tgatccagcg gccagtgcgc cgctcttgcc cggtgcagat ggcacggtgg
    35821 ccgcatttgg cgccactgtg gccgagaccc tgaagtcgat gacccagctc accctgccgg
    35881 tgccgacgat cgctcaactg cagaccgatt acatccagca ggccactgcg ctgtggaacc
    35941 agtccctgca gcagttccag gcgtttgcag ggtcggctct cgaccaatcc gcggccgctg
    36001 cggaaaagcc ggtgctggct gatcggcgct ttgctgcacc agactgggtt tcgaatccgg
    36061 ccagcgcatt cacggcccag ttctacctgc tgaacgcgcg cacattgatg aacatggccg
    36121 atcagttgca gggtgacgaa aagaccaagc agcgcatccg ttttgcggtg cagcagtggg
    36181 tcgacgctgc gtcgccgagc aactacctgg cgctgaatcc ggaagcgcag cgcaaggcac
    36241 tcgagaccaa gggcgagagc atcaagcagg gcctgcagca cctctggaac gacatccagc
    36301 aggggcacgt gtcgcagacg gacgaaagcc tgttcgaggt gggccgcaac gtggccactt
    36361 ccgaaggttc ggtggtgttc gagaacgaac tgttccagtt gctcgagtac aagcccctga
    36421 cggccaaggt gcacgagcgt cccatgcttt tcgtgccgcc ctgcatcaac aagtactaca
    36481 tcctcgacct gcagcccgac aactcgctga tccgctacac ggtggagcag gggcatcgcg
    36541 tgttcgtcat gagctggcgc aatgccgacg aaagcatggc gcagttcacc tgggacaggt
    36601 acatcgagga cggcgtgatc cggggcattg aagtcgcgcg cgacatcagc ggggcggatc
    36661 aggtcaacac gctgggcttc tgcatcggcg gaacgctgct gagcacggcg ctggcggtgc
    36721 tggctgcgcg tggcgagaag ccggcggcga gcatgaccct gctgacgacg ctgctcgact
    36781 tcagccacac cggcatcctg gacctgttca tcgacgagtc gatggttcgc atgcgcgaaa
    36841 cctcgatcgg gctggacagc ccggccgcgg gcggcctcct caagggccag gaactggcat
    36901 ccaccttcag cttcctgcgg ccgaatgacc tggtctggaa ctacgtggtc ggcaactatc
    36961 tgaagggcga gactccaccg ccgttcgacc tcctttactg gaacagcgat agcaccaacc
    37021 tgcccggtcc gatgtactgc tggtacctgc gcaacaccta cctggaaaac cgtctgatga
    37081 agccgggcga tctcaaggtg tgcggtgtgc cgctcgatct gtctctgatc gacacccctg
    37141 cctacatcta tgcctcgcgc gaggaccaca tcgttccctg ggatgcggcg tatcgcagta
    37201 cccaggtgct cgccggcaag ggcaagaagc gtttcgtgct gggagcctcc ggccacatcg
    37261 caggggtcat caacccgcct gccaagaaga agcgcagtca ttgggtcggt ggcactgccg
    37321 ccttgccggt cggtgcggat gcctggctcg acaaggcgcg tgagcagccg ggaagctggt
    37381 ggaccgactg ggcgcaatgg ctcgccagcc aggccggccg gcagattccg gcccccaagt
    37441 cagcagggaa ccgccgccac cctgtcatcg agcccgcacc gggcaggtac gtcaagcaga
    37501 aggcttgacg ccgaccggtt gcgcctcggc cgtttgcccc gcggttcgcg ttaacgtcga
    37561 gggttttctc aatcaactcg ggagttcatc atgagcacag acatcgtcat cgtttcagcc
    37621 gcccgcaccg gtgtgggcaa gttcggaggc agcctcgcca aggtatcggc ttcggcgctc
    37681 ggctcggtcg tgattgccga ggtgctcaag cgcgctggcc tcaccggcga gcatgtcagc
    37741 gaggtcatca tggggcaggt gctgcaggca gggacgggac agaacccggc ccgccaggcc
    37801 tcgatcggtg ccggcctgcc gaacaccgtg ccggccatga ccatcaacaa gctgtgcgga
    37861 tccgggctga agtcggtcgc gctggccgtc caggccatcc gcgacaacga tgccgaaatc
    37921 gtcgtggcag gcgggcagga gaacatgagc ctggcgcctc acgtgctgcc gggttcgcgc
    37981 gacggcgttc gcatgggtga ctggaaactg ctcgacacga tgctgtcgga cggcctgacc
    38041 gatgcgttca acctgtacca catgggtgtc accgccgaga acgtcgccaa gaagtacggc
    38101 atcagccgcg aggcccagga cgcctttgcg ctgtcgtcgc agatgaaggc ggcagcggct
    38161 caggaggccg ggcgcttcaa cgatgaaatc gtcaatgtca gcattcctca gcgcaagggc
    38221 gacccggtgc ttttcggcac cgacgagttc gtgaaccgca agtgctcggc cgaaggcctg
    38281 gccggactgc gtgccgcctt cgacaaggcc ggttccgtca ctgccggcaa cgcctctggc
    38341 atcaacgacg gtgcagccgc agtgctggtg atgagcgctg cacgcgctgc agccctcggc
    38401 ctgacgccga tggctcgcat tgccagttac gccagctccg gactcgatcc agccatcatg
    38461 ggcatgggcc cggtatcggc atcccagcgt gcgctcgcac gcgccggctg gaaagcctcc
    38521 gatctcgacc tgatggaagt caacgaggcc ttcgcggcac aagcctgtgc cgtgcaccag
    38581 gaaatgggct gggataccag caagatcaat gtgaacggcg gcgcgatcgc gataggtcac
    38641 cccatcggtg cgtcgggctg tcgcgtgctg gtgaccctgc tgcacgagat gcagaagcgc
    38701 aacgccaaga agggtctggc gagcctgtgc atcggtggcg gcatggggat tgcactgacg
    38761 ctcgagcgtt gaaccaggcc gggcggcgtc agtggcaacc cggttgaccg cgaggcgagg
    38821 gcgggtgacc ctcgtcgcct gagcgtgcgt tgtatgaatc ttaaggagcg agacaaatga
    38881 gcaagaaagt agcgtacgtg accggcggca tgggcggcat cggaacaacg atgtgccagc
    38941 gcctgcaccg cgacggcttc accgtgatcg ccggctgcgg cccgagccgg gatttcaaga
    39001 agtggctcga tgagcagagc gccctgggct acaccttcca tgcgtcggtc ggaaacgtcg
    39061 gcgactggaa ttcgaccgtg gccgccttcc agcaggtcaa ggccgaacac ggcccgatcg
    39121 acgtgctggt gaacaatgcc ggcatcaccc gcgacggcat gttccgcaag atgagccgtg
    39181 ccgactggga tgcggtgatc gagaccaacc tgaccagcct gttcaacgtg accaagcagg
    39241 tcatcgagga catgctggac aagggctggg gccgcatcat caacatcagt tccgtcaatg
    39301 gcgagaaggg ccagttcggg cagacgaact actcggccgc caaggcgggc atgcacggct
    39361 tcacgatggc gctggcgcag gaagttgcca acaagggtgt gacggtcaac accgtcagcc
    39421 ccggctacat cgcgactgac atggtcaagt cgatacgcca ggacgtgctg gacaagatca
    39481 tcgcgggcat cccggtcaag cgcctgggca cgccggatga catcgcgtcg atggtgtcgt
    39541 gggttgcgtc agacgaatcc gggtttgcaa ccggggccga tttctcggtc aacggcggcc
    39601 tgcacatggg ctgatatcgg gttgcagcgg ggcggtcagt cctgcagcaa gtccctcgcg
    39661 acgccagcca acgtgctggc gtcgccgttc tcgggctcta ccagtcgtgc gtcggcaaac
    39721 ccgatgaaat tgcgctgcat cgattgcagg tacaccgggt cgtagtgcgt gagcagcagt
    39781 tcccgcacca cgccctcgat gcctccggat cgggccgctt cctgccagcc gttgatcacc
    39841 tcgttgccac gcagcgcgcg cagtgcatcg aggcgggtgc agaaggtcgg cacgtcgtcg
    39901 acgaagaagg cgtagtcctc catcagcagg gagaccctgg catcgaccgg aagctggatg
    39961 tgcacgcagg gagacgctcg catgcactcg atcaggcgct cgggcacccg aagttcaccg
    40021 accttgcggc tctcgctttc cacccagacc gggcgcgccg gatcgaatcg gcgcatcgcg
    40081 tcccacagac gcgagtcgaa ctgcttctgc gacggttgcg ggctgccggg caccagaccc
    40141 agcaccgagc cccggtggtt ggcgagaccc tccagatcga ggacctgcgc gccctgcgcc
    40201 tgcaacgcgg ccagaaggcg gctcttgccc gagccggtcc tgccgcagat cgcgcgaaaa
    40261 cgcagcgcca ggggctgttc ctcgagcgcc ttcatcaccg cccggcggta ggcctggtag
    40321 cccccttcga gtacctgcac ccgaaagccg atcttgctga gcacgagcgc cagtgagccg
    40381 ctgcgctgac cgccgcgcca gcagtaaacc agtggctgcc agtccctggg cttgtcgagg
    40441 acgtgacgct cgatgtgcat ggcgatgttg cgggcgacca gcgcggcgcc gcgcttgcgc
    40501 gccacgaacg gcgagatctg cttgtattcg gtgcccacca gcgcccgttc ggcgtcgttg
    40561 aggctgggcc agttgactgc gccgggaatc cggtccagcg cgaactcgcc ttcggatcgc
    40621 gcgtcgatga tcgcgtcgta agcgtcgagc cgcgccaggc agtcgtcggc agtgaccgga
    40681 gtcagagcca tgggatcaat cctccgcgcc gcacggcatg tgcagtcccg tcatgctgca
    40741 atcgcatcga gcaattccgt ttcgatctcg atctgggtgc ggttgtgccg caaggccgcg
    40801 ccgtcgatca ggaaggtgtc ttccacccgc tcgccgaggg tggtgatctt ggccagttgc
    40861 aggttgatct ggtgtcgggc cagcacgcgg gcgatggcgt acagcaggcc cgagcggtcg
    40921 ctggccgaca cgccgagcaa ccagttctgt gcccgttcat cgggcctgag cgtgatgcgt
    40981 ggtgtgaccg ggaacgactt cacccgacgg gacaggcggc ctcgactcgg ctccggcagc
    41041 gctccgtcgg aggccagagc ccggccgagc tgggtttcca ccagcaggat caggtcgcgg
    41101 tactgggccg cgggcgctcc atcgcgcgac gcctcgagct gcggattgat cacctggaag
    41161 gtgtcgagcg cgcaaccggc gcgggtcgtg tgcaccttgg cgtcgaggat gttgaagtgc
    41221 gcgctgtcga agtagccgca gatccgggcg aagaggtcgg cgcggtcacg cgcatagacc
    41281 accacctgca gcccctcgcc aaccggcgac acgcgggcct tgaccaccgg cgttgctgcg
    41341 tcgaggtgct cgtgcagcga gcgggcatgc cacgcgatct cgccgacgtc gtgccgtgcg
    41401 aagtagctga tcgccagcgt cttccacaac gggacttcga cgtccggcgg ctgcagcgcg
    41461 aggttgagca tgtgcctggc ctcctgcttg cgcgcatcga tctcggcgtc gagatgcggc
    41521 tgcgcgccac cgagcgcccg cagcgaagcc cggtagaggt cctcgagcag cttgcccttc
    41581 caggcgttcc agaccttggg gctggtgccg cggatgtcgg ccacggtgag caggtacagg
    41641 gcggtcagcc gccgctcggt gccgacgcgc tgggcgaact cctggatcac cgccgggtcg
    41701 ctcaggtcct ccttctgcgc gacgcgggac atcgtcaggt gggcctggac gagaaactcg
    41761 atcagcgcgg tgtcgtcgcg ctcgatgccg tggtcgcggc agaagcggcg tgcctcgctg
    41821 gcgcccagtt ccgaatgatc gccgccacgc cccttggcca cgtcatggaa gacggccgcg
    41881 atgaagagca cccagggctt gtcccactgc agcgccagct gcgagcagaa cgggtactca
    41941 tgcacgtgct cgggaatgaa gaagcgacgc acgtttcgca gcaccatcag gatgtgctgg
    42001 tcgacggtgt agacgtgaaa caggtcgtgc tgcatctggc cgacgatgcg ccggaacacc
    42061 cacagataac gcccgagcac gctggtttcg ttcatcaacc gcagtgcgtg ggtgtgtccg
    42121 cttggtgccc gcaggatgcg catgaaggtc gcgcggttgg ccggggcgcg cctgaactcg
    42181 gcgttcatca ggtcgcgcga gttgtacagc gcacgcagcg tgcgggccga caggcccgtg
    42241 atgccgcgcg tggtctggta cgtcaggaag gtctcgagga tggcctcagg gcggcgctgg
    42301 tacagatcgt cgctgaccac ctcgagcatg ccgtcccgct cgaggaagtc ttcgttgatc
    42361 gggcgcatcg gcgcatcctg cgatccggtg attcgatcct cgatgttgag catcaggatc
    42421 tggttcagct gcaccaccgc cttggcggcc cagtagtagc ggcgcatcag cgcctctgat
    42481 gcgcgctggc ccttcgtcgc ccggtagccg aagctttcgg ccacggcgtt ttggaggtcg
    42541 aacaccagcc ggtcctcgcg ccggcctgcg accaggtgca gcttggtccg gatcagcttc
    42601 agcaggccct cgttgcgttg cagctgcttg gtctcgaacg gtgtggccag cccgctggcc
    42661 gcgagttcgg cccaggtccg tccgagtccg gctgcccgcg cgacccagat gatcagctgc
    42721 aggtcacgca ggcctccggg gctttccttg cagttcggct ccagcgagta cggtgtgtcc
    42781 tcgaatttct ggtgccgctg acgcatctcc agaaccttcg cccgcaggaa gttgcgtggc
    42841 tgcatcgcct gcgagttgtt gcgccgaaag acgttgaagc cgcggcgcga gccgcagatg
    42901 aagcgtgact cgaggagcgc agtgcgcacg gtgacatcgc gctcggcttc agccatgcac
    42961 tcctcgaccg tgcgcacgct cgatccgatc tcgaggccga tgtcccagca tgcggtgatg
    43021 aatgactcga ccgagcgctt ggcctcggac tggggactgc cgcttgcgca caggcccgaa
    43081 ggcagcagca cgagcacatc gacatccgaa tgaggaaaca gctcgccgcg tccgtatccg
    43141 ccgacggcca gcagcgccgc ttcggccggc atgccgcaga gctcccacag gttgagcacc
    43201 gtgacgtcgg tgtgacgcgc gattcccctg atcaagcggg tggctgcggg cgccgtcggg
    43261 cgcgacgcct cgaagtgacg gatcagcgcg tccttgccag ccttgtagcg ctgccgaagt
    43321 tcacccaggc agtcgggcgc cagcgcgccg tgctcatcca gctcgacaac tgacaaggcc
    43381 acaggcgctc aggccgccac ggattcgttg acgaactgcg gcaccgccgg actgccggcc
    43441 gagagggtca gcacttcata gccggtctcg gtgacgagca cggtgtgctc ccattgcgcc
    43501 gacagcgaac ggtccttggt gacgatggtc cagccgtctc cggcctcacg gatctcgcgc
    43561 cggccggcgt tgatcatcgg ctcgatcgtg aagatcattc ccggcgtcag cacttcgagc
    43621 gtgcccggct tgccgtaatg cagaacctgc gggtcctcgt ggaacttgcg gccgatgccg
    43681 tggccgcaga actcgcgaac caccgagaag ccattgttct cggcgaaggt ctggatcgcg
    43741 tgtccgatgt cgccaaggcg cgcgcccggg cgaaccttcg cgatgccgcg ccacatggcg
    43801 tcgtaggtca tctggcacag tcgcctggca gcgatcgatc cttcacccac cacgaacatc
    43861 cggctcgtgt cgccgtgcca gccgtccttg atgaccgtga tgtcgacgtt gacgatgtcg
    43921 ccgttcttca gcggcttgct gttcggaacg ccatggcaca cctgatggtt gatcgaggtg
    43981 cagatcgatt tcgggtaggg tgtatagccc ggcggcgcgt agttcagcgg tgccgggatg
    44041 gcttgctgga cgttcaccat gtaatcgtgg gccagcttgt cgagcgcctc ggtggtgatg
    44101 ccgggcttca catggggctc cagcatgtcc agcacttccg aggccaggcg gcctgcgatg
    44161 cgcatgccgt cgatgtcgtc ggcggtcttg atggtgatcg tcatggcgcc gattatctca
    44221 cccgacccgt aaaatcgagg gtctacccgt ggtggccgca gcgtcccgcg ggctatctgc
    44281 ctcgatcgtt gccgccctgt ctgccacccc ccaccccgag cgtcccgcgt gatcacttcg
    44341 cccaagcacc tgctccatgt cgaagggggc agtgcgctcc cggcctttcg cgcccaggcg
    44401 ctgctcgcga agctgcagca ggacgcctcc tgcggcggag cacgcatcac agcggtccac
    44461 gcccgacacg tgcactgggt ctggagcgag gcgccgctcg acccggatca gcgcgacacc
    44521 gtcgacggcc tgctccgcta cggagatgcg tatggcggcc cgagcgaagg agccttgatc
    44581 gtcatcgcac cgcgtctcgg cacggtctcg ccctgggcct ccaaggccac cgacatcgcc
    44641 cacaactgcg gcctggcgat caagcgcgtg gagcgagtca ccgaatacag gctcaccctg
    44701 aagaccgggc tgctcggtgg aagcaaaccg ctgacagttg acgagttgaa ggcagctgcc
    44761 gagttgctgc acgaccgaat gaccgaaagc gtgctgctgt gtcgggaaga tgctgcccat
    44821 cttttcgatg agcgcccggc tgcgccgatg gcccaggtcg acgtgctggg gcaggggcgg
    44881 gcagcgctgg tgaccgccaa cgtcgacttc ggcctggccc tttccgatga cgagatcgat
    44941 tacctggtca gcgcattcac atcgctgcgg cgcaacccga ccgacgtcga gctgatgatg
    45001 ttcgcgcagg ccaacagcga gcactgccgg cacaagatct tcaatgccga cttcaccatc
    45061 gatggggcgg tgcaggaccg ctcgatgttc cagatgatcc gcaacacgca tcaactggcg
    45121 ccgcagcaca ccgtgatcgc ctacagcgac aactcgtcgg tgatggaagg cggcgtcatc
    45181 gagcgctggt tgcccgaggg ctacaccgac gcgcctcgct acggtgtgcg cagcgaactg
    45241 gcccatgtgc tgatgaaggt cgagacgcac aaccatccga ccgcgatctc gcccttcccg
    45301 ggcgcttcga ccggagccgg tggcgagatc cgcgacgagg gcgcgaccgg ccgcggctcg
    45361 aagccgaagg ccgggctgac cggcttcacg gtgtcgaacc tcaatctgcc cggcaccgac
    45421 tggccctggg aacgttcgcc ttacggcaag ccggagcaca tcgccagccc ccttcagatc
    45481 atgatcgaag ggccgctggg cggcgcagcg ttcaacaacg agttcgggcg gccgaacctg
    45541 gcgggctact tccgcgtcta tgagcagacg gtcggcgttc ccggccacca gcagcgccgc
    45601 ggctatcaca agcccatcat gatcgcgggg ggactgggca cgatcgcggc gacgcagacc
    45661 gacaaggtcg agttcgcggc cggaaccttg ctggttcagc tgggcggccc cggcatgcgc
    45721 atcggcatgg gtggcagcgc ggccagttcg atggctgctg gcgccaacgc ggcggcactc
    45781 gacttcgact cggtgcagcg cggcaacccg gagatccagc gtcgcgccca ggaggtcatc
    45841 aatcactgtg ctgcgctcgg cgacgccaat ccgatcctgg cgatccacga cgtcggtgcg
    45901 ggcggaatct cgaatgcctt tcccgaactg gtcgacggag cgcatcgcgg tgcacgtttc
    45961 gacctgcgcc aggttccgct cgaggaaagc ggcatgtcgc ccaaggaaat ctggagcaac
    46021 gagagtcagg agcgatacgt gctcgcggtg cagcccgatc ggcttcccat gttcgatgcg
    46081 atgtgcctgc gcgagcgctg cccctatgcg gtcgtcggcg ttgccactga agcgaagcaa
    46141 ctggtgctgt cggacgaacc ggtgacgtcc gtcagggctc cttcgcttct tgccatcgac
    46201 atgcccatgg acgtgctgct cggcaagcca ccgaagatgc accgcgatgt gaagcgcgtg
    46261 cgtccgcagc ttggcgcgct cgatctcacg gatgtggatc tgcagacggt ggcgctcgat
    46321 gtgctccgcc acccgacggt cgcgagcaag cgtttcctca tcacgatcgg agatcggacc
    46381 gttggcgggc tcagtcaccg cgatccgatg gtcgggccgt ggcaggtgcc ggtggccgat
    46441 tgcgccgtga cgctggccga ttacagcggc tttcgcggcg aggcgatgag catcggggag
    46501 cgaacgccgc tggcagcgat cgatgcgccg gcctccggcc gcatggccgt cggcgaggcc
    46561 ttgaccaacc tgcttgccgc gccgttcgcg ctggagcgtg tcaagctcag ctgcaactgg
    46621 atggccgcgt gcggcgaacc gggtgaagat gccgcgctct acgacaccgt gcgcgcggtc
    46681 ggcatggagc tgtgcccggc cctcggcatc agcgtgccgg tgggcaagga cagcctgtcg
    46741 atgcgcaccc gctggagcga ccgcggcgag agcaggcagg tcactgcgcc ggtctcgttg
    46801 atcgtctcgg cgttcgtcac gctcgacgat gtgcggggaa cactgacacc gcagttgcag
    46861 ccgggtgata ccacgctgat cctgatcgat ctcggcaatg gcaggaaccg catggccggc
    46921 tcgatgctgg cgcaggtgct tgatcagacg ggcaatgcat ccgccgacgg cgtgcccgac
    46981 ctcgacgatc ctgctcagct gaagtcgctg atcgcagcca tcaaccagct ccgcagcgaa
    47041 gggcgcttgc tggcctacca cgatcgcagc gacggcggcc tgtgggcgac ggtttgcgag
    47101 atggcctttg ccggtcacct cggtgtgagc ctgaatgtcg acatgctggt gaccgaaggc
    47161 gacggcatca gcgacagccg catggactgc ggagattcga agaactgggc tggccagatc
    47221 ggtgcgaggc gccacgaact gaccttgaag gccctgttca gcgaagagct gggtgctgtg
    47281 atccaggtgc cgaccgcggt tcgcaacgag gtgatgcaga cgctgcgcgc tcacggcctt
    47341 tcgaagcaca gccacttcat cggcaagccg aacgacgccg gcagcgtcga ggtctggcga
    47401 gacaccaagg ccgtcttcag cgcaccgctt cgcgtcctgc atcaggtctg ggacgaagtc
    47461 agctggcgca tcgcccggct gcgagacaac ccgcactgcg ccgatgccga acatgattcg
    47521 gcggggctcg ccgaggatgt cggcctgcag gtacacctga cgttcgatcc ggcgcaggac
    47581 gtgtctgcga cgttcattca caccgcccgt ccgaggctgg ccatcctgcg cgagcagggc
    47641 gtcaacagcc aggtggagat gagctacgcg ctggcgcagg ccggcttcga cacctacgac
    47701 gtgcacatga gcgatctgca ggccgggcgt gcgcggctcg accagttcat cggcttcgtc
    47761 gcctgcggag gcttcagcta cggcgacacg ctcggcgcgg gcgaaggctg ggcgaggtcg
    47821 atacttttca attcgaagct cgccgatcag tttgcagcct tcttcggccg taccgacacc
    47881 tttgcgctgg gcgtgtgcaa tggctgccag atgatggcgg cgctgtcgcc catcatcccc
    47941 ggcgcgcagg cctggcccaa attcacccgc aacaagagcg agcagttcga ggcacgcctg
    48001 agcctggtcg aggtgatgga cagtccgtcg atcttcttca gtggcatggc cggaagccgt
    48061 ctgccggtcg cggtgtcgca tggggaaggc cgcgcggact tttcccagcg cggtgatgcg
    48121 tcagcggtcc accgtgcgat gcgtttcgtg gatcatgctg gtcatgccac cgaggcctat
    48181 ccgttcaacc cgaatggcag ccctgatggc ctgacgtcgg tcaccactgc cgatggccgc
    48241 tacaccgtgc tgatgccgca cgcggagcgg gtctttcgaa acgtgcagat aagctggacc
    48301 tctggcccgg caagcgagtc gagcccctgg gcgcggatgt tccgcaacgc gcgccgctgg
    48361 gtcgcctgac cgagcgtctc ccgcgcgcca gatgcgatca tcgaagttca tcccaggaga
    48421 cgcgatgcga tcttttttcc agattctggt tgctgcgctg gccctggcgg gatgtgcgca
    48481 gctgccgatg agcgatcccg tcaaggtcag tcttgtcggc atcgagccga tgccggggca
    48541 gggtctggaa ttgcgcatgg cggtgaagct gcgcgtgcag aacccgaacg aatccgaagt
    48601 cgcctacaac ggtgtggccc tcgatgtcga gttgaggggc ctgagtttcg ccagcggggt
    48661 gagcgacgtg cagggcacga ttcctcgcta cggcgaggcg gttctggtcg tgccggtctc
    48721 ggtctccgcg atatcggttc tgcgacaggc gctcagcttt gccaatggcg accgctcgaa
    48781 tgtcggctac gcgatcagcg gcaagatcgg aggctcgctg ctgcgtggcg aacgattcaa
    48841 gtccaccggc cagatcgacc tccccacgtc actgctcggc ccgcccgggc gctgacgatg
    48901 gcgcgccggg catcgcagcg cggttgccat gcgtgaagcc attgcagtcg gcgaagagcg
    48961 cttcgagcga ctggatgcgc tgcgcggcct ggccatcgtc tggatggcgc tgtttcacct
    49021 ctgcttcgac ctgaaccatt tcggattcat ccgtcaggac tttcacaacg aaccgctgtg
    49081 gaccgtccag cgcagcgcga tcgtcgggct cttcctgttc tgcgtcggac tgagtcaggc
    49141 cgtggctctg gatggcggtc acgacagatc ggcctggggc gggcgcttct ggctgcgctg
    49201 ggcgcagatc gcgggatgcg ccctgctggt cagcctcggt tcgtggtgga tgtttccgcg
    49261 cagctacatc agcttcggcg tgctgcacgg tattgcggtg atcctgctgc tgttgcgcct
    49321 ggccgcgcct gccggtcgct ggctctggtt gttcggcctg gctgcgctgc tgcttcccct
    49381 gtggttccag catgaattct tcgacagccg ctggacgaac tggatcggtc tggtcacggc
    49441 caggccgatc accgaggact acgtgccgct gctgccgtgg ctgggcgtgt cgctgtgggg
    49501 gctggcagtc gggcaatggg tcctgcagca tcgtccaact ctcctgtcat tgcctctgtc
    49561 gccgacgctg cgcccggcgg cccgtccgct tgcgtgcctc ggtcgctgga gtctcagctt
    49621 ctacatggtt caccagccgg tcctgatcgg cttggtggca gcagcggcat ggctggtccg
    49681 cacgccggcc tgagctgttt gaacccgccg aaacccaacc gctaccatcg cccgatgagc
    49741 aacaagatcc tgttcggctt tcatgccgtc accgtgcggc tgaagaccac gccgaaatcc
    49801 gtcgtcgaga tccacgtcga cacatcgcga cgcgaccagc gcatgaggca gttcatcgag
    49861 cgcgccaccg cggccggctc gaagctgatc gacagcgatg acgccaagct gcagaagatg
    49921 tgcggcacgc atcggcatca gggtctggtg gcccgggtcg agccggtcgc gctgagtcgc
    49981 tcgctcgatg acacgctcga cgaactcgac gctgtcgagg gcaacccgct gctgctggtt
    50041 ctcgacggca tcaccgaccc gcacaacctc ggcgcctgcc tgcgggtcgc cgacggggct
    50101 ggtgcacatg cggtgatcgc gccgaaggac catgcggtgg gcgtcaacgc gacggtctcg
    50161 aaggtcgcca gcggtgcggc tgaaaccgtg ccctacttca tggtgaccaa cctggcacgc
    50221 accctcaatg agctgaagga acgcaacatc cgcgtcatcg gcacatcgga cacggccacg
    50281 caatcgctct acgacatcga cctcacgggg ccggtcgccc tggtgcttgg cgccgagggg
    50341 gcagggatgc gacaactcac gaccaagacc tgtgacgaac tggtcagcct gccaatgcgc
    50401 ggcgcagtcg aaagcctgaa cgtctcggtc gccagcggcg tgtgcctcta cgaagcgctg
    50461 cgccagcggc gtgccgccga aggcaacctg gcctgaaacg gcgcgcgcgg gcggccgtcg
    50521 tttcgactca cgcctgggcc cagaacacgg cgaaccagcc gttcgcatcg acccagcagc
    50581 gggtgcgtgc gtatcccgag tcgtgaagca gttcggcgaa gctttcgacg gtgtacttgt
    50641 acgagttttc ggtgtgcatt cgatcgccgg cctcgaatcg cctctcgttc tcgggccact
    50701 gcacgaccac ggcgcgacgc gcctcgaggt gcatctcgat gcgcgagtgg tctcggttga
    50761 agaacgagac gtggcgccag tcccgtgggt cgaagttgct gccgatcaag cggttcacgt
    50821 gccgaagcag gttcagattg aacgcggcgg tcacgcccaa tgcgtcgtcg taggccgctt
    50881 cgagctgctc gatcggcttg accaggtcga tgccgatcag cagtccaccg ccggtagctt
    50941 gccggcgcac gcccgcaaga aagccttgcg cagatgccgg cgtgaagttg ccgatgctcg
    51001 accccggata gaagaaaacc gcacgcccct gaagcaggcc cggcggcaat tgcaccgtgc
    51061 tcgagaaatc gaggccgaca ccgcagacct cgatcgcagg gtgctcgcgc tgcacttgtg
    51121 caatcgcagg ctccagaaag tcgaccgaga tgtcgaccgc gacgtagcgc gacgggcgca
    51181 gtggtgggat gaccatccgt gccttggcgc aattgccggc ccccaggtcg atcagcgtgc
    51241 cgatgggacc gatggccgct gccatctcgg gggcgtggtc ggcgaatatg cgggcctccg
    51301 tccgcgtcgg gtagtactcg ggcagctcgg tgatggcctc gaacagcagc gaacccaggc
    51361 ggtcataaag aaacttgggc gaaagggtcg caggttcggc gctcaggccg gtgagcagtt
    51421 cccggtgcaa tgcctgtggg ttgtgctggt ggacggccac caggcgtgtc ctgggctgtg
    51481 cgtcggtggg ctggcctcgc gcgatgtcga gagtgggttc gctgctcacg ggtggcactc
    51541 catgcgatgt gtcggttccg aggccgagga cctttgcgca cattgtgggc gtgttccgtc
    51601 agcgttcgga tcgcgccccc gaattgcccg cgcggactga atggctgcat ccaccccgat
    51661 aatccgcgac gcctttgcct ccccgctgcc catgcagatc ggtttgtcca tcaacgaatt
    51721 cctgttttgc gcgatgctgg caaccggcct gtgccgctgc gcatggcgct ggaaggtccg
    51781 cgccatgccc gagccgccga agcggcagcc gttctgggcg atgctgggtg ccgacctgtt
    51841 cgtggtcgtc gctctggtct tcacgtgccg tgtcgccgtt gccgactggc cgcgcgtgcc
    51901 atcggaatcg atggagccca cgctgcgcgt cggtgactac ctgctgatca accatctggc
    51961 ctacggaccc aggctgcctt tcaccaacac cgcgatcgag ttcgcaagcc cgcagcgggg
    52021 cgacgtggtg gtgttccgct atccgcatga cgtgtcccag ttctatgtca agcgcctggt
    52081 cggcatgccg ggagatgtcg tcacgttcgc tgacggaacg gtgtccatcc agggcgccat
    52141 gcccgatgcg caggtgctcg aagacgatgc gctggcggcg ttctcgatcc ggcggtcggc
    52201 cgatgaccga ggccagtgga tggtgcgtga gtcggccggc gcaccgccgc gcgtgctgaa
    52261 gatcaatccc ttcgtgcgcg gtcgccagcc gctcaactgg gacgacgccg gcattgcggc
    52321 ccaatgcagc agcacgcgtg gcggtgcctg ggaatgcacc gtgcctgaag gtcactacct
    52381 gatgctcgga gacaaccggg atgcatcgtc cgattcgagg acctgggggt ttctgtcgca
    52441 cgatcaggtc tacggcaagg ctgttcgcgt gctcttcaac ttcgacgatc ccgagcgggc
    52501 ctggctgccg ctttgacact ggagctgtcc acatcatgat tgacaccccc gatccggacg
    52561 ccgacctgcg cggcaagcac atcgtgctcg ggctgtctgg cggcattgcc tgctacaagg
    52621 ccgccgaact ggcccgcgca ctggtgaagg caggcgccac ggtgcaggtc gtgatgaccg
    52681 aggcggccga gcagttcatc accgcagtga cgatgcaggc actgaccggt cgcgcggtgt
    52741 tcacgagcac ctgggatgcg cgcgctgtcg atggctcggc gaacaacatg gcgcacatca
    52801 acctgacacg tcaggccgat gcggtgctgg tggctcccgc cagtgccgac ttcatggcga
    52861 agctgctgca tggtcgcgcc gatgatctgc tcagcctgct gtgcctggcc cggccgatcg
    52921 aatcgtgtcc gctgttgatc gccccggcga tgaaccgcga gatgtggtcc cacccggcga
    52981 ctcagcgcaa tgtggtccag ttgcgcgccg acggtgcgca cgtgctcggc ccggccagtg
    53041 gcgagcaggc ctgcggtgag gtcggagacg ggcggatgct cgagcccgag gaactgctcg
    53101 aggaactgac tgcattcttt cggcccaaac cgctggccgg caagcatgtg ctgatcactg
    53161 ccgggcccac tttcgagccg atcgatccgg tgcgcggcat cacgaacctg tccagcggaa
    53221 agatgggttt cgcgatcgca cgcgctgcgg ccgaagccgg cgccgaggtc accctggtgg
    53281 caggaccggt ccacctgacc acaccgcgcg gcgtgcggcg catcgatgtc cgcacggcaa
    53341 tgcagatgtt cgatgcggtc gttcccgcgg ccgcgaatca ggatgtgttc attgcgaccg
    53401 ccgcagtggc cgactggcgg ccggcgagct tgtccgagca gaagatcaag aagaacggca
    53461 gcaagatcgc gccgatcttc gagctgaccg agaaccccga catactggca gcagtcgcca
    53521 gccaggccct cgctcgcggc cgtccgttct gcgtgggttt cgccgctgaa agccatgatc
    53581 tgctgaagca ttcgcgcgaa aagctcgtgc gcaagcaggt gccgttgatc gtcggcaacc
    53641 tgggcccggc caccttcggt cgcgacgaca acgcgctggt gctcgtcgat gctgacggcc
    53701 accgcgatct gccgcaagcc gacaagctca cgctcgcgcg ttggctgatt gccgatatcg
    53761 cgctgcggct ggcgcacgtg ccggatggaa agacaggaaa gccgcaatga cgacgatcga
    53821 tgtgaagctg ctcgatgccc gcatggctga ggccatgcca gcctatgcga ccccgggcag
    53881 tgcgggtctc gacctgcgcg cctgtctgga tgagcctctg ctgatcgagc ccggccagac
    53941 gaagctgatc cccaccgggc tcgcggtgca catcggcgat cccggcctgg ccgcgatgat
    54001 cctgcctcgc tcggggctgg gccacaagca cggaatcgtg ctgggcaatc tggtcggtct
    54061 gatcgattcg gattaccagg ggccgctgat ggtcagctgc tggaaccgcg gcgtggcgag
    54121 ctacaccgtg cagccgatgg agcgcatcgc ccagctcgtc atcgtgccgg tggtccaggc
    54181 cagtttccga cgtgtcgatg agttcggcgc atcggagcgg ggcaccggag gtttcggctc
    54241 gaccggcgcg aactgaactc gctgcgtgtt cgaacacgca gcacgcgtca gtgcaggtgc
    54301 gggcccggtg ggtcggcgtt gaacacgctg aatgcaggct ggccgacgct tccggcatcg
    54361 atctcgtccg acgtggcttg cctcacgtcc tgcaccgtca gatgcagccg caatgcgacg
    54421 ccggcgagtg ggtgattgcc gtccagcacg acatgggtcg ggtacacctc ggtgaccgtg
    54481 tagaccgcat cctcaggcat gcccggagtg accgagcctt caggcacacc ttcgaactgc
    54541 atgccgacct cgatgccgtc gggcaagacg gatcgctcct cgaagaacac cagatcggcg
    54601 atgtagtcgc cgaacgcatg ctcgggttcg aggtgcagcg atgcctcgaa gccggtggcc
    54661 tggccgagca aggacgcctc gaccttctcg agcaggtcgg cgccaccgta gaagaattcg
    54721 accggttgtg cgagttcatc gatcaactgg ccctgggcgt cttcgagtcg ccatgtcagt
    54781 gcgaccacgc aaggggattg gatttccatc gggaaatgat ctcacagggc gacttcgcta
    54841 tcgggctgca gtccatgccc cgcttcgagg cggtggacaa tccgcagcat gcgcatcgac
    54901 catcccacac ctctgctcgg cgggctcagt ccgcaggcct tcatgcggcg ttactggcag
    54961 aagaagccgt tgctgatccg tcaggcggtg cccgagggag ttgctgaact cgatcgtgct
    55021 gcgctgtttg cgctggcggc tgccgacggc gtcgaatcgc gcctgatcga gaggcgcgat
    55081 accggctgga agctgcgcca cgggccgttc gcccgacgcc agttgccacc gctggggcgt
    55141 cccggatgga cgctgctggt gcagggcgtg gacctgcatg cccaggccgc acatcggctg
    55201 atgtctcggt tccgcttcgc acccgatgtg cgtctcgatg acgtgatgat ctcctttgcc
    55261 accgacggcg gcggtgtcgg cccgcacttc gattcgtacg acgtgttcct gctgcaactc
    55321 gccgggcggc gtcgctggcg cgtggggcgc atgaagaatc cgacgctgca accggatgtt
    55381 ccgctgaaga tcctgtcgca cttcgagccc gaactggagt ggacgctcga accgggcgac
    55441 atgctgtatc tgccgcctcg ctgggcgcac gacggtgtgg ccgacggaga ctgcctcact
    55501 gcatcgatcg gcttccgggt tccttcgcgg gaggaggtcg gatgcgaggt actgcagcgc
    55561 atgctcgatg ccgtcgatga acgggaagaa ggactgcgct atcgcgatcc cacccagcct
    55621 gccaccgagc aacccgcccg cattccacag cccctggccg acttcgcggc cgatgcggta
    55681 cgtcggctgg tcgacgatcc ggactcgctg gcctgtgcac tgggggaatg gctcagcgag
    55741 ccgaagccgc agacatggtt cgacccggtg gatgaatggt ccggatctgg caccgtggtt
    55801 ctcgatcggc gcacgcgcat gctgtacgac gaacgtcatg tgttcatcaa cggcgagtcc
    55861 ttcatcgcgt cggggcgtga cgcgaagttg atgcgtgtgc tggccgatcg ccgatcgctc
    55921 gacgctaaat cagtggcacg actcggggca gacgcccggg agttgctgca gtcctggact
    55981 gaccagggct ggcttcatgc ggccgacatc gctgattgac ggactgcgct ggcgtcggac
    56041 agactcgaca gcctgagttc attgacagga acagcgcacc atgagcacgc ctgcgagcca
    56101 tttgatccaa agccgaaccg agttccagga tgccttgcga cgcgccttcg ccgacattgc
    56161 ggcggccggg gcgcgcgaaa tctggatgtg cgacgcggat ttcgcggact ggccgctcaa
    56221 cgaacccgag gtggtggaga acctgtcgag gtgggcgatg ggacatcgaa cctgcaccat
    56281 cgtggcgctc agctatgacc agatcgtgcg ccgtcacccc cgatgggtgc agtggcgcag
    56341 agagcgctct cacatggtgc ggtgccgaac accggacgag gcagatcggg tcgatgtgcc
    56401 ctgcgtgctg ctggcccccg gcacgctctc cttgcgcctg gtcgaccgcg atctgtaccg
    56461 cggcagcgtg tcgacagcga tcgccgatgc gacgcgcgat cgcgatcgcc ttgatgcact
    56521 gatgcaacgt tccgtcgacg cctttcctgc aagcacgctg ggtttgtgaa ccagtacgca
    56581 gactgagaca tatacttgcg ggctgtagca aaagggcttt ttggcctttc gctcctgagt
    56641 ttcgggccga ttgcatcagc agtgacccca gaccaccttc atttatcgac cttttttgat
    56701 tgaggataaa catgaacaag acgctcgtgc tggctaccct ggttgctgct gtcgccctcg
    56761 ctgcttgcgg caagaaagaa gaaatggcgc ccgccatgga agcagcctcg gctgctgcag
    56821 caccggctgt ggccgcggtt gaagccgccg ctgcgtccgc agtcgacgca gctgcttctg
    56881 cagcagaagc caccgttggc gctgccagcg gtgccgcgac cgaagccgct ggtgcagctg
    56941 ccgacgctgc caaggacgct gccaacaagg ctgccgacgc cgccacggac gcaatgaaga
    57001 agtgatcggt cctcacggac gagaaaaagc cgccctcggg cggctttttt gttggcgcgt
    57061 gccggcagat gccctgtggc atcgcctgca ccgcagctag atttacttca gatcgttgac
    57121 cagcagggtc gcgatacgag cgggcgtgtc gcccttctcc gtccccgcct tctcggtcag
    57181 cacgctgact gtgctgcgct cgccttcgcc cttgacctga acacggtagc gcgcgagctt
    57241 gtcggggtcg ttcttgtcct tgccgaagcc caggagcttc ccgaagaatc caggctcttc
    57301 cttgttgtac ggcttcggat cggcgtaccg cacgtagtag atgccttgga tgcggtcccg
    57361 atcctcgacc gtgaatccgg tgcggtccag cgcgacgccg acacggcgcc aggctcgatc
    57421 gaaaccttcg tccacttcga tgaccgatgt ctgtcctgcc accacgcggg cccgcggagc
    57481 ctggggctcg gtggttgcca tcatggcctt cgcctgatcc tccttcgcgc cgagcttcaa
    57541 cagcaggcgg cgcagcattt cggcctcgag ctccggatcg gccgggcgcg gctgccagac
    57601 ggtgctgtcc ttcagcgcgc cggtggccac ctcgaccatc ccgcgatggc tgatgaagat
    57661 atcgctgccg ccggccgagc gctcgacccg ggtgcggtac ttgtcgcgct cacctgtcga
    57721 atagatggcg tcgaacacct tgccgatcgt gttgcggatg aagtcctgcg gcaacctggc
    57781 gcggttctcg ttccattcgg tttccatcac gccgacatcc gcctgattga ccgtcaggtt
    57841 gaatccgcgc tcgcgccaga actcctgcaa gacgggccac agttgttcag gcgtcatcgt
    57901 cgtgaccagc cagcgctcgt tgccagcgcg gtcgatgcga agggctgcat cacctgccgg
    57961 cgcgaccgcg gcaccggtcg agagattcga cggcgacgac tggtaagccg ctgcgctgaa
    58021 cgcgccggaa tcgtcctgcc tgtagcgcga ttcgcgggac agctgcgtga gatcgggcgg
    58081 aatctcgagt cccgccgtgg cggttgcggc agacgccttg taatcgacct tgtcgccatg
    58141 catcaaggtg tccacgctcg aacagccggc catgccgatc accagtgccg agcaggccag
    58201 acgccgtgca ttcgagcgtc tgagggctag tcgattcgac cgggtgggtt gggaagctgt
    58261 cacgttgttc atctccgtgg ggttggcggc ggacatgccg cgcagcccgt tgatcgcagt
    58321 gggcgaggga agggccgagc ccggggcttt gcgttgccga cgatgcatcg tcagattgtt
    58381 agatcaggcc ggcatcgcgc agggccgatt cgacggtggc ttgtccggct gcggtcaggg
    58441 gcatcagtgg aagccgcagc gtgttcctgc agcgccccaa ctgggccagg gcccatttcg
    58501 tcggcgcggg gctgggttca gtaaacaggt gcttgtgcag cggcagcaga cgcatgtgga
    58561 tctcggtcgc ttcgcgcacc cggccctcca tggcggcaac gcacagctcg tgcatcgagc
    58621 gcggcgcgac attggccgtg acgctcacat tgccgtggcc tccgagcagc atcaaagcga
    58681 tggcggtcgg atcgtcaccc gaatagatcg agaagcccgg cggagcgtgc ttgatcaacc
    58741 atgcagcgcg ttcgatgttg ccggtggctt ccttgaccgc gacgatgccc ggcaactgcg
    58801 ccaggcgcag cgtggtttcg ggctgcatgt ctgccaccgt gcggcccggc acgttgtaaa
    58861 gcaccagcgg aagatcgacc gcctcggcga tcgccttgaa gtgctggtag atgccttcct
    58921 gcgaaggctt gtt
//
LOCUS       DAOORV010000008        58046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_590_length_58046_cov_9.358952, whole genome shotgun sequence.
ACCESSION   DAOORV010000008 DAOORV010000000
VERSION     DAOORV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 58046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_590_length_58046_cov_9.358952"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKV56_02250"
     CDS             complement(<1..230)
                     /locus_tag="PKV56_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX66659.1"
                     /translation="MAIEKVRQIFSNPDSDTNIMSAWEDYLSGEPCRSDALRRLIDDS
                     WRRCHSAQVDPGRDSAPPPLSEDCLHSLLDDHG"
     gene            complement(666..1259)
                     /locus_tag="PKV56_02255"
     CDS             complement(666..1259)
                     /locus_tag="PKV56_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOX66660.1"
                     /translation="MPYTLQTPVHSEQLIRKSRFIACVQPIPGRTEALAVVAALRAQH
                     PGAAHVCWALLAGGQSAAVDDGEPGGTAGRPMMDVLRHQDLDGVLATVVRYFGGVKLG
                     AGGLLRAYTDAVAQALIGAVKVPLQRLQTLRCSVPYALEGLLRREIHSAGHTLLDVKH
                     GDSVELWISLPETQVAPFVHRIDDAGQGQVQWLSMQD"
     gene            1420..1722
                     /locus_tag="PKV56_02260"
     CDS             1420..1722
                     /locus_tag="PKV56_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66661.1"
                     /translation="MVPITLFDELSESIIKSTGTLDLASGEIRAIQYEDHDLDTQGLP
                     ADDEDYEFTSGMLSHRGKEIEFRVDVDVLTGKYSVTPSELLELKGRAAKLFTAPPG"
     gene            complement(1744..4299)
                     /locus_tag="PKV56_02265"
     CDS             complement(1744..4299)
                     /locus_tag="PKV56_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX66662.1"
                     /translation="MLALLKHLTWPEWRHHPWRQAAALLAVTLGVALAFSVQLINQSA
                     LSEFSAAVRSINGQPDFELRGQRGGFDETLYERVARHPDIALASPVVEIETQALTIDA
                     QGATKRVPLRVLGIDALVAAPLSPALLPNLSAPRGSEGEPDRLGLFDPDAVALNATAR
                     QRLGRIDTLRLQTPDGIVALRVHGSVAAGGPPLVVMDIAGAQVQFGMLGKLSRIDVRL
                     KPGADRRKVVAALALQDFAAQGVRAASPDEAAQRISNVSRAYRVNLTVLALVALFTGA
                     FLVFSILSLSVAQRQPQFALLGVLGLSASQRLRLVLAESALIGVAGSVLGLALGTALA
                     AAALQLLAGDLGGGYFPGVTPTLHFSVTAALLYGGLGVVAALVGGWLPARAAQRLAPA
                     QALKGLGDASDLEAVAWFGPALIAAALALLLLPPIFDMPLAAYLSVALLLLGGIVCVP
                     LGVGLLLRAIAPPKQALALLAVERARHLRSTATVAVAGVVASLALSVALTVMVASFRD
                     SITAWLDTVLPADLYARAGASTGGSDVITLAPGWVRDAARVDGVIRVETLRVSSVQID
                     PAQPAVALIARPLADPARSLPLVGDLVAMPAGETAAYVSEAMVALYGASPGTRLMLPL
                     PTGQRARVFVRGVWRDYARQHGAVAIASDDWQTLTGDVRINDLALWLRPGTDPSAVQQ
                     KLRAMAGRHGMDGELLEFASPGEIRAISLRIFDRSFAVTYWLQGVAIAIGLFGIAASF
                     SAQVLARRKEFGLLAHLGLTRRQVLTVVTAEGAVWTAAGALLGLALGLAVSVVLVKVV
                     NPQSFHWTMDLMLPWARLAALCAAVIAAGTLTAGLAARHAAGRDMALAVKEDW"
     gene            complement(4304..4975)
                     /locus_tag="PKV56_02270"
     CDS             complement(4304..4975)
                     /locus_tag="PKV56_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66663.1"
                     /translation="MPLLDVRSLSKRYGTEPVFSNVDLQVARGEFIAILGESGVGKST
                     LLNCIAGLDTVQAGSVHIAGTDITALSEPAQALFRREHLGFVFQAFHVLPHLSVAHNV
                     GLPLLLQHQPDAARVHEVLQAVGLDALADRLPQTLSGGQLQRVAIARALVHRPQLILA
                     DEPTGNLDPATADKVMDLLSAQVRQQGAACVLVTHSRAAAARADRVLTLTPAGIVEAE
                     RAPSG"
     gene            5141..5656
                     /locus_tag="PKV56_02275"
     CDS             5141..5656
                     /locus_tag="PKV56_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66664.1"
                     /translation="MVPESSLRRAFPLICAALWLAGCGTAPSPSAPSKGTVVPRPTLA
                     QERQRIGDLFQGTPVTLAMDGDSSLRAEVPLKFSFDPGRAVVKPPLAALLDRLAGSPA
                     TRGGSWVVAAPGDPKNKGNSLALERAASTRDYLVGRGADPLRFSVSALPGVGEAALIR
                     IVVKAPPSASR"
     gene            complement(5687..6658)
                     /gene="miaA"
                     /locus_tag="PKV56_02280"
     CDS             complement(5687..6658)
                     /gene="miaA"
                     /locus_tag="PKV56_02280"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOX66665.1"
                     /translation="MPVANRGILCLAGPTASGKTAAALAIARAFAERQPSLPVEIISV
                     DSALVYRGMDIGTAKPSAAELAAVPHHLIDLIDPSEAYSAARFVGDAQRLIGEIRARG
                     HLPLLVGGTMLYFKALFEGLDAMPAADRAVRAALDEQCERQGLHALYRELQRVDPVTA
                     ARLAPGDRQRIQRALEVHRVSGQPLSSFHQDKTPVETPPLISLEPGDRAWLHQRIEAR
                     FGQMLEAGFVDEVQALMERGDLHADLPSMRCVGYRQTWEALQADDLSDLPERGIAATR
                     QLAKRQLTWLRSMPQRHVVPCDAADAQAQVVALALRLADTLPARPAG"
     gene            complement(6838..7158)
                     /locus_tag="PKV56_02285"
     CDS             complement(6838..7158)
                     /locus_tag="PKV56_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66666.1"
                     /translation="MTTSDLPAATRGTLAYRTFFCCALACTVIAVTYFLIGMADGSVS
                     SFNLGLWLLLLGGLGVVLWTGHLLRRSGRTGAAIAVLALVAVPGLFAGLLVLIVAVTQ
                     PRWN"
     gene            complement(7254..8702)
                     /locus_tag="PKV56_02290"
     CDS             complement(7254..8702)
                     /locus_tag="PKV56_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX66667.1"
                     /translation="MATLKIKPKNPAAPAARIERTPVRGSGMGVRRRPTLAEAQADRA
                     RRDAAPPFDRPERAPPARKPFQSHSGPAADPRDAPRPPRASRPPERSAEPRRDPRNEP
                     RGEPRQDRRDGPPPRPSTRDREGTAERQRFAGSQAGRPPQERAQRRPEPARGQEAQQR
                     DRPPIIAQPRFPVSPPERPPERPSARPLERPFERPPQRPPEPKWRQAERAERHPDDEE
                     RGPFHQQPEAAPGSLRLSKRMSELGLSSRREADEWIEKGWVRVDGEVVKELGSRVLPE
                     QRITVDARAKFQQAQKVTVIVNKPIGYVSGQAEDGYEPAVVLVKPENQWTDDQSGTRF
                     LREHLRSLVPAGRLDIDSIGLLVLTQDGRIAKQLIGEDSEIEKEYLVRVAGPLGQGPA
                     SSELSPDDLELLRHGLELDGEALKPAIVEWLNDDQLRFVLQEGKKRQIRRMCEAVGLK
                     VIGLKRVRIGRVMLGDLPVGQWRYLRADEVFG"
     gene            complement(8769..10661)
                     /gene="mutL"
                     /locus_tag="PKV56_02295"
     CDS             complement(8769..10661)
                     /gene="mutL"
                     /locus_tag="PKV56_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOX66668.1"
                     /translation="MNARGPLTPRRPIRELPDELISQIAAGEVVERPASVVRELVDNA
                     LDAGASEITVKLTGGGIRSVLVEDDGQGLPAAELPLALRRHATSKIESLSDLENVSTM
                     GFRGEALAAIASVAEVSIASRCADDPKARRLDARSGEITPAARGIGTSVEVRELFFST
                     PARRKFLKTESTELSHCVEAVRRHALTRPDVSFAVWHEGRLVQQWRRAEAEARLRDVL
                     GTEFIADSRAVALDLGVLRVTGRAGTPASARSRADQQYVYVNGRFVRDKLIAHGVRSA
                     YEDVLHGGRQPAYVLFIDIAPDRVDVNVHPTKIEVRFRESREVHQAVRRAVEASLAEA
                     IAGVEATTRTEAVEMLPGTFGQLAAAPDPTGPVAWGRSDARNDRYGGTPTSAQVALSL
                     HQATVLFGNEPAAPWGSAGAPGHDGTRAGLSLVHLAKAATHDESARPLGEAIAQIAGI
                     YVLAENAQGLVIVDMHAAHERVVYERLKASLAASRIEAQPLLIPATFAATTAEIAAAE
                     AHGDTLRSLGLDVSPLSPTVLAVRSQPAALAGGDVVELARSVLSELGEHDASSVVQRA
                     QHEILSTMACHGAVRANRQLTLDEMNALLRDMERTERADQCNHGRPTWRQITRRELDA
                     LFWRGR"
     gene            10755..11411
                     /locus_tag="PKV56_02300"
     CDS             10755..11411
                     /locus_tag="PKV56_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOX66669.1"
                     /translation="MEIVSVLIDFILHVDVHLANFVQTYGAWVYALLFAIIFVETGVV
                     VMPFLPGDSLLFVVGTLCGAGLMSLPLAIAVLLVAAILGDQTNYSIGRHFGPKVFQWE
                     QSRFFNKAAFEQAHAFYEKYGGITVVLARFMPFVRTFAPFVAGVAAMSRTTFTLYNVV
                     GALIWVVGLTVAGYVFGNLPFVKQHLDKIIWALILVPGLIAIWGARKARSKVTLPASG
                     "
     gene            complement(11589..12272)
                     /locus_tag="PKV56_02305"
     CDS             complement(11589..12272)
                     /locus_tag="PKV56_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX66670.1"
                     /translation="MNPAVTAPCQPSTARSPLLIKAACAIVLTAALGHATAGVVISEL
                     GTGAPGATLGGYTMTPFGDDGIGSFTDILSLASPLGGNLGFSNSVNTRAVGNGWATWS
                     HGYAGDVYFNLSQSLTITLPNDVSAFYLYVESNDFDLFDFTLTAQDGSTSTESVNGNA
                     GAFGFGIYGTDGTELTSLTITASGDSGGFAVGEFGIAKSAEVPEPASLALVGLALAAL
                     GVSRRRKTV"
     gene            complement(12543..13904)
                     /locus_tag="PKV56_02310"
     CDS             complement(12543..13904)
                     /locus_tag="PKV56_02310"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOX66671.1"
                     /translation="MGAVSLTLVGVSGTHAAAIVAVRVWPADDYTRVTIESDSALAAR
                     HFLAESPTRLVIDVDGLDLSPQLRDLVGQVRADDPFIAGVRVGQYQPRVVRLVIDLKQ
                     AIKPQLFALTPVAAYKHRLVFDLYPVQERDPLLALIQEKERAEEQAAQSVRDALGEFI
                     GQVDKPLGTAIPPDGASAPPKGAPTPAGKPLGMTPANPPDGPSVRPPESPASAASRIS
                     RLVIVALDPGHGGEDPGAVGPSGLREKDVVLSVALQLRNRLNALPGMRAMLTRDADFF
                     VPLKDRVRKARRVQADLFVSIHADAFFTPEARGASVFALSQGGASSAAARWMANRENL
                     ADAVGGINVKSKDSTVMHALLDMSTTAQIKDSLRLGGEVLGQIGRVGRLHKARVEQAG
                     FAVLKAPDIPSILVETAFISNPEEEAKLRDVRYQAQLVEALTVGIKRYFAKNPPLARA
                     RRT"
     gene            complement(13967..14458)
                     /gene="tsaE"
                     /locus_tag="PKV56_02315"
     CDS             complement(13967..14458)
                     /gene="tsaE"
                     /locus_tag="PKV56_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOX66672.1"
                     /translation="MPILEACTALWPDEAACAATARELARHAGLRNAVIELHGTLGAG
                     KTTFVRHLLRALGVSGHVKSPTYAVVEPYALPGLDIWHFDFYRFDDPQEFEDAGFRDV
                     FAGPGLKLIEWPERAAGLLPPCDLRIDIDMVDAPASDPDADDTRRRATFRAGTPLGAT
                     LMP"
     gene            14503..15660
                     /gene="queG"
                     /locus_tag="PKV56_02320"
     CDS             14503..15660
                     /gene="queG"
                     /locus_tag="PKV56_02320"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOX66673.1"
                     /translation="MVAGHGLDESLLWPRVQAWAGELGFSQIGVADVDLSSAEPGLQA
                     WLDAGFHGSMDYMQRHGLKRARPRELVPGTVRVLTARMDYLPAESDPPGPPGDWQTVE
                     WQRLQAPERATVSMYARGRDYHKVLRNRLQALADRIAAAVGPLGHRVFTDSAPVLEVE
                     LAARSALGWRGKHTLALSRDAGSMFFLGEIYMDLALPVTGPVAAHCGSCSACVDVCPT
                     RAIVAPYRLDARRCISYLTIEHAGPIPIEFREAMGNRIYGCDDCQLVCPWNKYAQRST
                     LADFDVRAVLADPTLLDLWRWSEADFLRHTEGSPIRRIGHERWRRNLAVAMGNAVRAL
                     PESELCSALRGALAQALPDASELVREHIEWALLQSAAKATDEPRSPATPAR"
     gene            complement(15620..16519)
                     /locus_tag="PKV56_02325"
     CDS             complement(15620..16519)
                     /locus_tag="PKV56_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOX66674.1"
                     /translation="MSHALLLVPDLLLIVVGFLLCRHTLLDRPVWDAVERLTYFLLFP
                     VLLFSSIAKSPLHPAQTAALAGVGVGTVLCGIGLAYAIRRWPGVDARLHASGAQTAFR
                     FNSYIALALAERLGGAETLALMALLIALCVPICNIGAVWPLARHGGHAYVRELVRNPF
                     ILGTLAGLLFNLAGLHLPDAVGTTLHRLGLAAIPLGLMAVGAGLKLGGLQAAPALAAA
                     FLTIRHVALPAIALALSIWVGLPPLQQAVVLAFAALPTASSAYVLAMRMGGHGPFVAG
                     LVTMSTLLGMVSVPVWLAIGARL"
     gene            16551..17453
                     /gene="xerD"
                     /locus_tag="PKV56_02330"
     CDS             16551..17453
                     /gene="xerD"
                     /locus_tag="PKV56_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOX66675.1"
                     /translation="MSSLRDSTAAIDRFIDALWIEDGLSANTLAAYRRDLTLYAHWLA
                     AGPDVALSGTTETHLRDYAVARHEGSKASSTNRRLTVFKRYFRWALRERLIDADPTLK
                     LLAAKQPPRLPKVLSEAQVEALLAAPDVTTPLGLRDRTMLELMYASGLRVSELVTLKT
                     VHLGLVDGALRITGKGAKERLVPFGEEARAWLERYLAEARADILKSQASDALFVTARG
                     GPMTRQMFWRLIKQHALRGGVHVPLSPHTLRHAFATHLLNHGADLRAVQMLLGHSDIS
                     TTTIYTHVARERLKTLHAAHHPRG"
     gene            17530..18921
                     /locus_tag="PKV56_02335"
     CDS             17530..18921
                     /locus_tag="PKV56_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOX66676.1"
                     /translation="MKVTASNFKAYDIRGIVGQTIDEAFAEHLGRAFGSEAVKAGERA
                     VAVGRDGRLSGPGLAAALIRGLMSTGLDVVDLGAITTPMLYYVAATRGRRGCHSGIMV
                     TGSHNPKNYNGFKMVLAGRAIYGDDIQNLRVRMEAEDYVTKAGRSAPMDIGPEYSARI
                     TGDCKLKRRMKIVVDSGNGIPGASAPGILRALGCDVTELYSKVDGDFPNHHPDPSKPE
                     NLADLIRTVQTTDAEIGLAFDGDGDRLGVVTKDGNIIYPDRQLMLLAKDVLSRNPGGT
                     VIFDVKCTQRLAPVIRKAGGKPLMWKTGHSLVKAKMKQVKAPIAGEMSGHIFFGERWY
                     GFDDATYTAARMLEILSRSKDPSKVLNALPTSYSTPELNVPCAEGEPKLLVQKLLETA
                     KFPGALEIITIDGLRAEYKDGFGLIRSSNTTPVLVMRFEGHTEKALHRIEAAFMAALR
                     VVKPDAQIAAAAH"
     gene            18967..20271
                     /locus_tag="PKV56_02340"
     CDS             18967..20271
                     /locus_tag="PKV56_02340"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOX66677.1"
                     /translation="MQRLARVAYSTLLRLALPAYVLKLWLRGRAEPLYRFAIGERFGW
                     YRVDRRSDPPSSGWVWVHAVSLGETRAASALVNALRDLRPGMRLLLTHGTATGRTAGA
                     ALLRPGDRQAWLPFDTPGSVARFLAHFQPAIGVLMETEIWPNLLLEASRRGIPTVLAN
                     ARLSERSARKARRFEALFRPVAESVPLVLAQTQDDADRLRAAGARRVEVCGNLKFDVT
                     PDAAQLARGSAWKLACGRRIVLAASTREGEEGPLLAGWNQQPAPRPLLLIVPRHPQRF
                     DEVAALVESSGLTCWRRSLWADAPPADAGTADVWLGDSIGEMAVYFSCARVALLGGSF
                     EPLGGQNLIEAAACGCPVLMGPHTFNFSQAADLALHEGAAQRVADIDEAVQVAVGMAS
                     EPAGAAGRSPDVRPSPQRALEFAARHRGAAERMARQISALLG"
     gene            complement(20300..21658)
                     /locus_tag="PKV56_02345"
     CDS             complement(20300..21658)
                     /locus_tag="PKV56_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOX66678.1"
                     /translation="MAIAVHGGLASRGGLRVKRVCTGLLFAAGLCAAQAQSLDELYKA
                     AHDYDATYLSARSLAESAQYRAAQADALLRPSLNLGANANIGRVDTALGTASFNNRAL
                     QMAGRQPLFNRASSASIDQAQRELDAASLRLETAEQDLMVRVAQAYFDVLAAKDALAT
                     TRTSKVAINEQLASAKRNFEVGTATITDTREAQARYDLATAQEIAADNDLRNKRIALD
                     QLVGRSNVEPLGLKPPVTLPPLSPADAEAWAGQADVQHPSVRLAQLSLEVAQLETRKA
                     QAGHLPTVDVVGSLSDSYSSGGSGGSQLSRSGSTKSASIGLELNLPLFAGLSVQNRVK
                     ETLVLEDKSRNDLEAARRAVSQGTRQAYFGVQSLQAQVLALEAAESSSALALEATQLG
                     YRVGVRVNIDVLNAQTQLFTTRRDLSKARYDTLLGGLRLRQAAGQLKPDDVAAVSRLL
                     GP"
     gene            complement(21690..22031)
                     /locus_tag="PKV56_02350"
     CDS             complement(21690..22031)
                     /locus_tag="PKV56_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX66679.1"
                     /translation="MQHIGAREFAALLAEASSTDLPLLLDVREPWEFERAALRAEGLT
                     TLNLPMSEIIQRIGELDPARPTICLCHHGVRSAQVAAYLEQRQGFASVTNFTGGIDAW
                     STEVDASVQRY"
     gene            complement(22031..22708)
                     /locus_tag="PKV56_02355"
     CDS             complement(22031..22708)
                     /locus_tag="PKV56_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOX66680.1"
                     /translation="MDTELARFNMIEQQIRPWDVLDADVLALLAAVRREDFVPAAYRS
                     MAFVDTEIPLHTAQGPAQSMLAPRVEARLLQELAVGKHERVLEVGAGSGFMAALLGHQ
                     AQQVLSLEIDAELAVFAADNLRRAGSSNVTVRHADGSKGCPGEAPFDVIVLSGSVPQV
                     PASLFDQLKPGGRLVAVVGQLPIMRAVLYTRSAGSPTDGAVASVDLFDTVAPRLTGFD
                     EPSRFQF"
     gene            complement(22782..26015)
                     /locus_tag="PKV56_02360"
     CDS             complement(22782..26015)
                     /locus_tag="PKV56_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX66681.1"
                     /translation="MWFTRVSIGNPVMATMVMLAFVVLGLFSYQRLSVDQFPNIDFPT
                     VVVQFDYPGASPEIVETEVTKKVEEAVNTVAGISSLYSRSYEGNSIVIIEFNLNIDGR
                     KAAEDVREKIALVRPSLRDEVKEPRVSRFDPASTPIFNIAVLSPSGKQSPQELTTWAT
                     QVLQKRLENVRGVGSVSVVGGVSRQINLYLKPAAMEAMGVSVDNVINAVRSENQELPM
                     GAIRSAEQERVVQVNARLKRPEDFREIVVARRGAATPGTGNATVSGGSPVKLWQVADV
                     VDGPQEVESLALYNGQRTVLLSVLKSQGENTIEVVDGLRKALAGAESVTPPGVVVEVN
                     RDNSRAIRVAVANVKRTLIEGAALTVLIVFLFLNSWRSTVITGLTLPIALIGTFGFMY
                     VFGFTINSITMMALSLCVGLLIDDAIVVRENIVRHVQMGKSAHQAALDGTQEIGLAVM
                     ATTLSIVAVFLPIGFMGGIVGKFFHEFGLTIVAAVLISMFVSFTLDPMLSSIWHDPQA
                     SHLPGAPRGTSSLYDRTLGRVTEQFERFTVRLGNGYQAILRWSLRHRLSTLAIAMGTF
                     GGSFMLIPLLGAEFVPKADFSETTINFYTPVGSSLEMTEARARQVDAALRELPEVRYT
                     VTTINSGNAAGKIFGSTYVRLVDRAERQRSLDEMTVPMRERLARIPGVTMTNIGTPDL
                     GGGKSLQFSIQGSDLGELERLSKVITEQLHRVPGLVDLDSTLKPDKPTVAINVRREAA
                     SDLGLNVNSLATSLRSLVAGTTVGNWRAPDGENYDVIVRLNPESRNSMADLERLPVPV
                     VAGADGMPRIVRLSQVADVSPSTGPNQINRRDLNREINMDANAYGRSSGDVSADIRRI
                     LDGIAWPPGYRYSFGGSTKNMTESFNYAVGALGLAVVFIYMILASQFRSFLQPIALMS
                     SLPLTLIGVVLTLMMFRSTLNMFSIIGIVMLMGLVTKNAILLIDFAIRARAGEVPGEP
                     ATAAGHLGREEALLLAARVRLRPILMTTLAMIFGMVPLAFALSEGSEQRAPMGQAVIG
                     GVIASSLLTLVVVPVVYCYLDDLSAWAKRRFLRSTQQAVPVPLIQGK"
     gene            complement(26034..27221)
                     /locus_tag="PKV56_02365"
     CDS             complement(26034..27221)
                     /locus_tag="PKV56_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX66682.1"
                     /translation="MKRISLGWVVLILAVLLVAGFIGRTLYGRAQERALAAAPAKPPP
                     ALELGAGDLVVARTQTFQRTLAITGGLKAVDSAVVRAKVAAEVKSLTVREGDTVKAGQ
                     TIGQLDTLEVDLRVQQADQNAASARAQLDIAQRALNNNRALVAQGFISTTGLETSISN
                     EAAARATHEAALAALNLARKSRTDTRLVAPISGLVSQRLVQPGERVAIDAKLVEVVDL
                     SRIELEAAVTPEDVGGVVVGQTARLSIDGLAAPATARVVRINPSTQAGTRAVMVYLAV
                     DPQPGLRQGLFATGSIDLRSSNVLAVPVSAVRVDQAQPYVLAVVGDRIEQRKVTLGQR
                     GELLVDGSVEAAVELTTGVTEGTVLLRGSAGAVRDGSLVRIAGGGASGSPATAASAPV
                     DAR"
     gene            27534..28985
                     /locus_tag="PKV56_02370"
     CDS             27534..28985
                     /locus_tag="PKV56_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX66683.1"
                     /translation="MLSLDRFSFQQLLLVAFLLIAGLLSAASLRGLFTIEALITQSSQ
                     GARQAVSLSAAAQRLAERSVTMERAARQFLVLDDPVLRQRFDDGAREAGEALTQLVAG
                     TDAAPRRDAAAAGWRRTLQTVRQQLVGEPDTLRDREQTLTAAFRELGQINIDLADQVR
                     VATEVRNATLLERLEQGRAALARQIIVTIAVAVGLALAFGIWLARPLKRLEAAIVGLG
                     ENRLDRPIEMRGPADVRQLGLRLEWLRLRLAELDADKARFLRHVSHELKTPLAAMREG
                     VSLMEDGVTGPLNESQREVARILRQNTAVLQAQIEDLLRFNTAAFDARRLVRRPVELR
                     SLISEQVEMQKLQWQARRLTVAVRGETLTVEVDAEKLGTALGNLLSNAIRFSPIGGTI
                     VFSVSQWPGRARIDITDQGPGVAPADRARVFEPFFRGERQPPDAARGSGIGLSIVQEL
                     VRAHDGQIDLLMPEAAEVEGAHFRIELPHAPAR"
     gene            29293..29709
                     /locus_tag="PKV56_02375"
     CDS             29293..29709
                     /locus_tag="PKV56_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66684.1"
                     /translation="MLPSDLAQEINRLAASPPAPETSLELALALMQTRNGGELNRAIG
                     LVEPLARGGSADQRAWQPFARLLLSRLLELRRLEELLDKRSQELRDSQREVRQLNEKL
                     EALKAIERSLAPRPSSALPPSTLSGTASFAPTRRTP"
     gene            29709..31115
                     /locus_tag="PKV56_02380"
     CDS             29709..31115
                     /locus_tag="PKV56_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX66685.1"
                     /translation="MPANLLVVDDDPDLLQLLSMRLTAAGYRVTGVGSAEAALAHLEA
                     VRPQLVVSDVQLPGRDGLALFDEIRARHPALPVILLTAHGTIPDAVEATARGVFTYLT
                     KPFDGKALLDKIAQALALGAPEAHREGPDEAWRAPIVSRSAVMAEVLAEAKMVAASDA
                     SVLICGESGSGKELLAQAIHRASPRAGKPFVAVNCSAIPEALLESELFGHVKGSFTGA
                     VANHRGLFQAADGGTLFLDEIGDMPPALQVKLLRVLQERSVRPVGANQSIPIDVRILS
                     ATHRDLDAAMAQGQFREDLYYRLNVVALTLPALGDRREDIPLLANHFLSKLAEKYGKR
                     LNGFAPEALKALTAAAWPGNVRQLHNVVEQVCALTTTPLVPLALVQRALRVPSVEVLS
                     FAQARERFERDYLVGLLKLTDGNVADAARLADRNRTEFYRLLQKYGLTPGLFRNEPRP
                     DGTLDPAPPATLSPIGDK"
     gene            31320..33593
                     /gene="mdoH"
                     /locus_tag="PKV56_02385"
     CDS             31320..33593
                     /gene="mdoH"
                     /locus_tag="PKV56_02385"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HOX66686.1"
                     /translation="MNHRLSPRLNRSWTEAGTGSGAAARRDVGASMPPMNRGSMVARP
                     WRGVIGGLIVGWIALMDRLAGRPAVPDTDSPAPPMPADAGATAAWKRSAVRRRRALLA
                     LVALSVVGATAMLDQVLPTYSSDALRTAQIALFSVLFGWVSAGFYTAMMGFWVQWRGD
                     PHALSSRSVLNRPIAAGARTALVMPICNEQVSTVFAGLRATVESLIAAGGSRLFDVYL
                     LSDTSDPTIRTAELAAWAELREAIGSVRIHYRWRQRRTQRKSGNVADFCRRWGRNYRY
                     MVVLDADSVMTGDCLLTLVRLMEAHPDAGIVQTAPQASGLSTIHARAQQFAGRVTGRL
                     FSAGMQFWQLGESHYWGHNAILRVEPFMKHCGLAPLAGRGGLSGHIMSHDFVEAALMR
                     RAGYHVWLVHDLAGSYEQQPPNLLEELQRDRRWCQGNLQNARLIAEPGLHGVHRAMLL
                     TGALAYLSAPLWLGYVLLGVALWLFGGHPSFSGDAAVPFEMLMLWAGTGVMLVMPRAM
                     GVVTILMRREQSQYGGGAALIRGALMEGLLSVLQAPLRMVAHTLFVVVALTGLKLDWK
                     SPPREAADIGWADASNRFALLGAAMLALAGAALALEPATLLWLAPVGVPLLLAVPLAV
                     LTSRAGLGERVRSLGLLVTPEETRAPTVLREAANYAFQNARNRSIKLDWRDAIDNPWL
                     FGVVRAAMGSRNTMWGHRGRARRQLVGRLRATHDIDSLSTADRMRLLSEPQHLVRLRD
                     QLMARTDPSTASWGRVG"
     gene            33780..34637
                     /locus_tag="PKV56_02390"
     CDS             33780..34637
                     /locus_tag="PKV56_02390"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOX66687.1"
                     /translation="MDIPLLIKAALMGIVEGLTEFLPISSTGHLILAGSLLDFTGETA
                     KVFEIAIQTGAMFAVIWQYRERLQRTVGGLGSDPVARRFAINVLIAFIPAVVLGLAFG
                     KAVKAHLFHPVPVAMAFIVGGFVILWVESRHKRLYGQLDLQGGRRARVETVDDMGPLD
                     ALKVGLLQCLALIPGTSRSGSTIIGAMVLGFSRKCATEFSFFLGIPTLMGAGAYSLWK
                     QREALHAGDLPMFAVGLVFAFFSALLCIRWLIRFVSTHDFVAFAWYRIVFGGIVLLTA
                     WQGWVNWAD"
     gene            complement(34660..35199)
                     /locus_tag="PKV56_02395"
     CDS             complement(34660..35199)
                     /locus_tag="PKV56_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOX66688.1"
                     /translation="MTRAASSLRVEPLTAEAFAPFGEVLEAGEAARHWTINAGNTERF
                     HDLAHLDPGADGRVIASIFRGAPRSLPFTVAVMERHPHGSQAFMPLFGGSSADRAVRT
                     YLVVVAPRGPAPTARQLRCFIAQPHQGVNYAAGVWHHPLLALRETSDFLVLDREGPGD
                     NCDEVDVDPPAVIELPARD"
     gene            complement(35225..36235)
                     /gene="alc"
                     /locus_tag="PKV56_02400"
     CDS             complement(35225..36235)
                     /gene="alc"
                     /locus_tag="PKV56_02400"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HOX66689.1"
                     /translation="MANPIIDPNAPEFTRRCTNLADPRLGAQVVSCSDEFFAPRERLL
                     APTDPVFVAGKYDDHGKWMDGWETRRKRGPGHDWCVVRLARPGRIFGVDIDTRHFTGN
                     YPPAASLEVCRSTHEVPDGTAHWAPLLPTVALAGNAHHHHAIDNHEVWTHVRLAIYPD
                     GGVARLRIYGRPEVDAVASGGALDLAAAINGAWVVAANNQHFGQASALLLPGRGVDMG
                     DGWETRRRREPGNDWCIVALAAHGVIERVEVDTAHFKGNYPDSCSLQAANVPGHATES
                     SLITQSMFWPELLAPQKLGMDAEHRFDTLAALGPVTHVRFNIFPDGGVSRLRLHGRIV
                     LL"
     gene            36331..37284
                     /gene="puuE"
                     /locus_tag="PKV56_02405"
     CDS             36331..37284
                     /gene="puuE"
                     /locus_tag="PKV56_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HOX66690.1"
                     /translation="MSLHADPPYPRDLKGHGPDVPHARWPGGARIAVQFVLNYEEGGE
                     NSPLHGDATSEVFLSEIIGAQPFENRHMSMESMYEYGSRAGVWRILREFDARGLPLTV
                     FGVGMALQRYPELVAAFMSRGHEIANHGWRWISHQHMPEATEREQIAMGTQVLKQLTG
                     GVWPQGWYTGRDSPQTRRLVVEHGGYEYDSDHYGDDLPFWMPVTCADGTVLPRLVVPY
                     ALDTNDMRFAQAQGFNTADHFFSYLRDAFDALYAEGDPDGLDRPKMMSVGMHCRLLGK
                     PGRIGALQRFLDHVLAHGAVWVARRIDVARHWARVHPCSSG"
     gene            37824..38699
                     /locus_tag="PKV56_02410"
     CDS             37824..38699
                     /locus_tag="PKV56_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX66691.1"
                     /translation="MKPSDIVDMTVLAALWGASFMLMRFASPAFGPIPLAALRVIGAS
                     LFLLPLLLMRGQAGELRKHWRPLLVVGITNSTLPFICFSYAALSITAGMSAIFNAATP
                     LFGAVIAWWWLRDGLTRQRVAGLAIGFSGVLWMAWDQASFKPGGTGWAVVVCLAAPLL
                     YGLSASYTKRFLTGVPSLTVAAGSLLAAALLLCVPAVVWWPAVAPSIADWGAAVFLAL
                     ACTALAYVLFFRLIANVGPARTITVTFLIPAFALLFGWLLLDEALTPRMFGGCAVILI
                     GTGFATGLLTLRARR"
     gene            complement(38723..40207)
                     /locus_tag="PKV56_02415"
     CDS             complement(38723..40207)
                     /locus_tag="PKV56_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX66692.1"
                     /translation="MREPAFRITTAAALSLTLLASGCALVGRDYQAPPPKLDANFIAA
                     GVSKLNTEPVAADIAAFWRGFNDAELSALVERALQANGDIRIAQARLQESRALREEVD
                     KSTLPSIGVEANANRSISPPSSVFQSSRSQRTGANFSAAFVANWELDFFGVERRAKES
                     AAAQAGASEASLGAVQTSVAAEVARNYLEVRGLQLRHAVTQASLANQRESLAIAQARV
                     DAGRGTQLDLERALTLTASTEAALPSLLAAIDLGIYRLATLSAQPPAELRARLATPAP
                     LPGLPVTDLAALPLGTPQQWMARRPDVAAAERQLAAATAEVGVTEGELYPSISLSGLL
                     GLASTRLGDLTDSQSRRYSVGAGITWTVLDFGRIRSRIAASEARTLQSLANYEQTLAT
                     ALEETEGSLAQFSRNAQRRESLARAERSASEASRLARLRYEAGVTDFLAVLDADRELL
                     STRDALAQADTSNATSLVAVYRALGGGWTAPTPVAATASGEAAR"
     gene            complement(40200..43445)
                     /locus_tag="PKV56_02420"
     CDS             complement(40200..43445)
                     /locus_tag="PKV56_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOX66693.1"
                     /translation="MNISRFFIDRPIFAAVLSVVITLIGAISIFTLPISEYPEVAPPQ
                     VVVRAQFPGANARVIAETVATPLEEQLNGVEGLLYMNSLATADGAMTLTISFKIGTNP
                     ESAETAVQNRVQRALPRLPEIVRQLGVTTEKSSPNLTMVVHMVSPDNRYDALYLRNYA
                     ALNVRDQLLRIPGMGSVQTFGSGDYAMRVWLDPQKLAARSLTSGDVVAALREQNAQVA
                     AGVVGASPSPAGTEFQLSINTQGRLTTEQQFADVIVKADPIDRSLIRIKDLGRVEMGP
                     NTYALGSLLDNKEASAIAIFQAPNSNALQLSADVRATMERLKADFPAGVSYSIVYDPT
                     RFVQTSIEKVISTLIEAILLVVIVVVVFLQTWRASLIPLLAVPVSIVGTFAVLQLLGF
                     SINTLTLFGLVLAIGIVVDDAIVVVENVERNIEAGLSPHDASVKAMQEVSGPIIAIGL
                     VLCAVFVPLAFVSGLSGQFYKQFAVTIAISTVISAFNSLTLSPALSAILLRPHDAPKD
                     AVTRGIDAVFGRFFRWFNRVFHRNADRYSGVVTTTVVRRKSFALLIYAVLLGLTVFMF
                     TKVPPGFVPQQDKQYLIGIAQLPEGASLERTTKIIREMSDIALAIPGVESSVAFPGLS
                     INGFTAASNSGIVFVTLDDFEKRTSPETSANAIVGQVNQRLGGIEGAFVFVLSPPPVS
                     GLGNAGGFKLQLQDREGAGEAALNGVVQQLMGRVYGNPDSTITQAFSNYQINVPQLFA
                     NVDRTKAKQMGVDLNNIYDTMQTYLGSLYVNDFNRFGKTYQVIVQADASYRAKAEDIA
                     NLKIRNNAGEMVPLGAMMTVEPTFGPAAVGRYNSLYTADFNGAPKPGFSSGEAQAEME
                     KILKETLPRGMGYEWTELVYQEKIAGNTMVYIFPLCVLLVFLVLAATYESWTLPLAII
                     LIVPMCILSAVTGVWLSGFFGQPADNNIFTQIAFVVLVGLACKNAILIVEFARELELQ
                     GRSPVQAVVESCRLRLRPILMTSIAFIAGVIPLVLSSGAGAEVRQAMGIAVMSGMIGV
                     TVFGLVLTPVFYAVLRVLSPARLHHAGRVPTTLAHAPDAAAALPAAGDSHA"
     gene            complement(43456..44673)
                     /locus_tag="PKV56_02425"
     CDS             complement(43456..44673)
                     /locus_tag="PKV56_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX66694.1"
                     /translation="MNIAASRPALSVSLSSALIAILLIGGCSKQGAPAGAPPGAPPVS
                     VAAAVQRTVTDIEEFTGRLEAPRSVEVRPRIGGTIDKVHFRDGARVAQGAMLFTIDPK
                     PFQAELARAQSQLSAARSRAALATSELARAKKLLDAKAVSAQEFDQLTSGAATSSADI
                     AAAEAAVRVAQLNLGYTSVRAPMAGQLSRAIITEGNLVNDQSVLTTLVATQTVYAYFD
                     GSEQTFLRLRKGAGGQQLVRMGLADEAGFPHEGKIDFVDNQLNPQTGSIRMRGVFDNA
                     RGQFTPGLFARLQLVGGAAYPAVMTPDRAIGTDQSRKLVFVIGEGNVAQAREVHPGTL
                     IDGMRVITSGLKAGELVVVDGLQRVRPGAPVTPIKVETDERGMPLPPAPAQPGAPGAA
                     PAAPASAASAAQS"
     gene            complement(44894..45517)
                     /gene="trmB"
                     /locus_tag="PKV56_02430"
     CDS             complement(44894..45517)
                     /gene="trmB"
                     /locus_tag="PKV56_02430"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOX66695.1"
                     /translation="MGSGQVRAMNEWGPIYTVPYTAGLLNAPRTFGRTAPLVFEIGFG
                     MGDATAAIAAALPDTDFLGVEVHAPGVGALLKLIGERGLTNVRIVRHDAVEVLEHMVA
                     PASLAGVHVFFPDPWHKTKHNKRRLIQPPFVSLLASRLSPGGYLHCATDWQPYAEQML
                     EVLLAERSLKNTADGYAPRPDYRPLTKFENRGLKLGHGVWDLVFRRC"
     gene            complement(45843..47654)
                     /locus_tag="PKV56_02435"
     CDS             complement(45843..47654)
                     /locus_tag="PKV56_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX66696.1"
                     /translation="MYFRQDESAGEESLQHPGPGPQVAAPASVGGEQQASYLRHCAAI
                     VESSDDVIISKSLTGIIESWNPAAERLLGYSSEEMIGKPMRSIIPEDRPDEEPSILTR
                     IAAGERVEQLETVRRRKDGRLINLSVNISPIFNTDGIVIGASQIARDITDRFRNQKLM
                     WLQANFDNLTGMPNRRNFAEKLEEILKDAAGKSQRIAVLFIDLDHFKQINNVMGRNAG
                     NQAIAMAAARIRESIRKKDSVARFGGDEFGVVLSRAESDDDIDQVTARLMLHLKEPME
                     VGDERIFLSCSIGVSVFPQDGEDADDLIRHADLAMYQAKRAGRDRAHYYEHPLEASAQ
                     HRLRLADALHRAVEQNQFSMVYQPIVDLRTGQICKCEALIRWNHPERGFISPVQFIPV
                     AEEIGLMHAIGDWVFGQAIRQLKVWQQQFGSDFQVSINMSPSQLQGIADSADNWVTEL
                     ARTGIAGDSVIIEITESMMVHPDHATASKLRTLRAAGMHLAIDDFGTGYSCLANLNKL
                     DASFLKIDQSFTRTIQDGSRDLALCQAIVSMARSLGLSVVAEGVETAEQMDLLGKIGC
                     DFAQGYLYARPLDAPGFERFMEQARSAQSHACLIGQD"
     gene            48309..48680
                     /locus_tag="PKV56_02440"
     CDS             48309..48680
                     /locus_tag="PKV56_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66697.1"
                     /translation="MPKTILIVDDSASLRTVIKLALARDGYDVIEAANGLEGVARLEM
                     TGKVHLILGDVNMPDMDGIAFVEHVKAHPRHGATPVIMLGTAGQDARKEQARAAGAKA
                     WFTKPFNTSQLLGAVGKLVLP"
     gene            complement(48755..48828)
                     /locus_tag="PKV56_02445"
     tRNA            complement(48755..48828)
                     /locus_tag="PKV56_02445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48794..48796),aa:Gly,seq:ccc)
     gene            49059..51077
                     /locus_tag="PKV56_02450"
     CDS             49059..51077
                     /locus_tag="PKV56_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX66698.1"
                     /translation="MRLPVLSIRSTVLLGIAMAVLAPTITLWHLDERLTRAAQEPLIA
                     QNRQAVLVMTSASLVEPMWTLDEEKTRQIAQKVLEEASVLSLRLTEGRPLSVPTVLAR
                     QGSELGQGVALKTTISREGENLGELEIWFDPDQIERALADRRSATILLAALQVLLSVV
                     VLLAVLYRRLLVPIGQLKRQASDIASRADVGPIAWDRRDELGELGEHLNEVHVHIDQL
                     FDQLEEQKAELEKVALHDPLTGLANRALFGELTRAAVAAAQRDGGRLALLFIDLDRFK
                     AVNDTLGHAAGDSLLVALAERLRGAMRASDVVCRHSGDEFTVLLRDVNHWDEVASTAD
                     RLLQEIEKPVYIGSRLAGVSASIGVALFPDDASDHETLVSHADTAMYVAKSLGRGRCS
                     FFRAEFNTQLLANLQLEQEMRQALERDEFVLFYQPQVDAGTGVLSGCEALIRWRHPVR
                     GLVPPLEFIPAAEQCGLISEIGAWTIRTACAQIARWKRSGISFGSVAVNVSALEFRSH
                     RLLDTLTSAMADFGVQPHELEIELTETVLMTDTDTSQVIVDRLHALGLPLAVDDFGTG
                     YSSLAYLKRLHPSKIKIDRSFVRDLPDDSDDRVLVQAIVQLAAAMGIQVIAEGVETQA
                     QRSFLHGIGCGLLQGYLISRPQEADGFERFVRQRAFERNAVLPEPIDA"
     gene            complement(51105..51581)
                     /locus_tag="PKV56_02455"
     CDS             complement(51105..51581)
                     /locus_tag="PKV56_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HOX66699.1"
                     /translation="MTLTLGDHLPTVPLEPSDQRLALLTESHQRLTGRVLVASEADLR
                     AALWNAPCVIVAHGTEPDPIFFYGNRRALEVFEMDWASFVGMPSRLSAEPLLREERAR
                     LLQRVTEDGCITDYAGIRISATGRRFRIEQAVVWNLIDGSGMCHGQAATFDRWQRL"
     gene            51767..52675
                     /locus_tag="PKV56_02460"
     CDS             51767..52675
                     /locus_tag="PKV56_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX66700.1"
                     /translation="MSDACVHVLQLGPGHAAEALARADPGYGPFQVTACSAGDVWERL
                     AAARVDVLIVQGAWTDWTRCASDVAVVLVVDQVSAADCLTWLQEGAQEVVTAAELVDP
                     SWSRRVRAAVERQRVRAATRQAFATDVHTGLPHVGQLIEHMSHLLALREREPAPMALL
                     VLRIEGLATTMARLGIEAAGTLRRKVAVRLRAGVRASDVVASVGDDSFAVLLSQIDTP
                     ADAERVGRKLLGSLGETFKVGGSDLAVATALGIAVYPSDGTQPDMLLRRAAGLAASAQ
                     ATGRSGYSNRLERGAAGSAANDEAPN"
     gene            complement(52683..53600)
                     /locus_tag="PKV56_02465"
     CDS             complement(52683..53600)
                     /locus_tag="PKV56_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HOX66701.1"
                     /translation="MNIPSLEIRHWVLLTFIASALYVHLRGRVRFRLVRALTDFTVFL
                     APVNVLMYLFSRTQRSPYIELAEFPEMAPLQQNWQTIRSEALRLNDEGYIRAAAGYTD
                     MGFNSFFRTGWKRFYLTWYGKDLASAEALCPQTVKLLRGIPSIKAAMFASLPPGATLV
                     RHRDPYAGSLRYHLGLQTPNDPGCYIEVDGQRYHWHDGEAVMFDETYIHHAANTTGQP
                     RVILFCDVERPLWGSPLRWFNRFFAKHVMAAASTQNVAGEPIGGVNKVFAQGYKLRAM
                     AKEFKRGNRTGYYVGKWLMIGALVWAIFW"
     gene            complement(53608..55086)
                     /locus_tag="PKV56_02470"
     CDS             complement(53608..55086)
                     /locus_tag="PKV56_02470"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOX66702.1"
                     /translation="MDNHTSPLAALTDPTLLKTDAFIDGVWVSAADRFVVDDPATGRK
                     LADVANLGAAGAEAALAAAERAWPAWRVKTAKERAAILMKWFHLLHQHADDLARLMTA
                     EQGKPLAEARGEVVYGASFIEWFAEEAKRVYGETVPTTDVNKRYLVIKQAMGVCAAIT
                     PWNFPIAMITRKVAPALAAGCTVVIKPAEQTPLSALALAELAQRAGLPPGVLNIVTAD
                     SERSIAIGRVLCASDTVRHLSFTGSTEVGRILAAQCAPTIKKLSLELGGNAPFIVFDD
                     ADIDSAVEGAMVSKYRNAGQTCVCANRLYVQDGVYDAFVEKLAAKVRGIQLGNGFDAG
                     VTQGPMIDANAIAKVESHVADAVAKGARLLAGGQRRDSRFYEPTVLADATGDMLCARE
                     ETFGPVAPVFRFHTEAEAIAAANDTEFGLASYFYSRDIGRVFRVGEALEYGMVGVNTG
                     LISTAEVPFGGVKQSGLGREGSHHGIDDYVEIKYLCLGDIQR"
     gene            55290..57938
                     /gene="alaS"
                     /locus_tag="PKV56_02475"
     CDS             55290..57938
                     /gene="alaS"
                     /locus_tag="PKV56_02475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOX66703.1"
                     /translation="MKATDIRQTFLKFFESKGHAIVPSSPVIPGDDPTLLFTNAGMNQ
                     FKDVFLGFDRRPYTRATTSQKCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFK
                     HGAIQYAWELLTEVYKLPKDRLWVTVYAEDDEAYEIWNKTVGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCSEIFYDHGPDVAGGPPGSPEQDGDRYIEIWNNVFMQFNRT
                     EDGVMHKLPKPSVDTGMGLERITAVLQHVHSNYEIDLFVNLLDAAKRAVDAAGAADCD
                     KDSPSLKVIADHIRACTFTVVDGIIPGNEGRGYVLRRITRRAIRHGYKLGARTPFFHT
                     LVAALAVEMGDAYPELRQQQQRVTEVLKQEEERFFQTISNGMEILEAALAETAAKPGE
                     AGRILNGDLAFKLHDTYGFPLDLTADVCRERDMTVDGAAFDIAMNRQREQARAAGKFK
                     MALGLEYSGTATTFHGYDHLVHEGAKVTAIYLDGTPALRARAGDDAVIVLDHTPFYAE
                     SGGQAGDTGELRNARSRVLVEDTIKVQASVFGHHGRIVEGEVEVGDSFTARVDAERRV
                     KTVRNHSATHLMHKALREVLGAHVQQKGSLVNAERTRFDFAHNAPMTEAQIAKVESLV
                     NAEVLANAATQARVMAIDDAQKTGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVTRTG
                     DIGLFKIVAESGVAAGVRRVEAITGDNALVYLQSLEGTMNQLAGTLKASPAEVPTRLT
                     QVLDQVRALEKELVAIKGKLASSQGDELLSQAVDVGSLKVLAARLEGADAKALRETMD
                     KLKDKLKSAVIVLASVEGGKVQLAAGVTADHIGKVKAGELVNFVAQQVGGKGGGKADM
                     AMAGGTDPSGLSAALYSVRAWVAERV"
BASE COUNT         9133 a        19316 c        19991 g         9606 t
ORIGIN      
        1 ccgtggtcgt cgaggaggga gtgcaggcag tcttcggaca ggggtggcgg cgcgctgtcg
       61 cggccgggat ccacttgagc ggagtggcag cgtcgccagg agtcgtcgat cagccgacgc
      121 agggcatccg aacggcaggg ttctccgctc aggtaatcct cccaggcgga catgatgttg
      181 gtgtcgctgt ccggattcga aaagatctgc cggactttct cgatggccat gctcagcgtt
      241 cctgctcgtc ggcgaatctg gggtgggcga attgcacgat gccatcgcta ccggaggtgc
      301 agaaccgcat tccggctctc ctgccaagac cggaggcgcc cggtcggcgc aagggccgcg
      361 ttcgtgccat gtcgggtact gaaccggccg tgaccgtcgg gctgcgcagg tgaggcgatt
      421 ccgtgcccgg catgcaacta gggtgccaag tgaggaagcc tcggcgggca gcagtcaagt
      481 ctttgatttg cttgtggatt ctcgacatcc ggctgattct cgctgctcgc agggtgtccg
      541 gaatatcgaa cacctggcca tgcggatcgc tggacaactg cgttcgacgg gcgcggcgca
      601 ggtgccggag tgctttgacc gactgcaact gcggtctccc ccgagccgga agcgaccgcc
      661 gtgcatcagt cctgcatcga cagccactgc acctgtccct gtcccgcgtc gtcgatccgg
      721 tgcacgaacg gtgcgacttg agtctcgggc aggctgatcc agagctcgac gctgtcaccg
      781 tgcttcacgt cgagcagcgt gtggccggct gagtgaatct ctcgccgcag caggccctcc
      841 agcgcatagg gcacgctgca gcgcagggtc tgcaagcgct gcagcggcac cttgactgcg
      901 ccgatcaggg cctgcgcgac ggcatcggtg taggcgcgca gcaggccgcc ggcgccgagc
      961 ttcactccgc cgaagtagcg caccaccgtg gccagcacgc catcgagatc ctggtgtcgc
     1021 agcacgtcca tcattggccg acccgcggtg cctccgggct caccgtcgtc gacggccgcc
     1081 gactgcccgc cggcgagcag cgcccagcac acatgcgccg caccagggtg ttgtgcgcgc
     1141 agggccgcca cgacggcgag cgcttcggtg cggcccggga tcggctgcac gcacgcgatg
     1201 aaacggctct tgcggatgag ctgctcgctg tgcacggggg tttgcagggt gtagggcatc
     1261 ggttgcaagc ctacccggac tcgacgcctc ttcggcctgc cagacacggg gggccgcgaa
     1321 gcaacgacaa tgcgggccgc gcctcgcagg ctgtcccgct gggggaaggc cgtgcggcag
     1381 gcgcgcagtc aacgggttag gagatccgac ttgaacctga tggtgccgat cacgttgttc
     1441 gacgaattgt ccgagtcgat catcaagtcg accgggacgc tcgatctcgc cagtggcgag
     1501 atccgcgcga tccagtacga ggaccacgac ctcgacacgc agggcctgcc cgccgacgac
     1561 gaggactacg agttcacctc gggcatgctg tcgcaccgcg gaaaggagat cgagttccgc
     1621 gtcgatgtcg atgtgctgac gggcaagtac tcggtcaccc ccagcgaact gctggaactg
     1681 aagggccgcg ccgccaagct gttcactgcg ccgccgggct gagcgagccg tctcgccggc
     1741 gcctcaccag tcttccttga ctgccagcgc catgtcgcgg cctgcggcgt ggcgtgctgc
     1801 caggccagca gtcagcgtgc ccgcggcgat cacggcagcg cagagtgcgg ccagccgcgc
     1861 ccacggcagc atcaggtcca tcgtccagtg gaagctctgc gggttgacca ccttcaccag
     1921 cactacgctg actgcgaggc ccagcgccag gcccagcagc gctccggcgg cagtccagac
     1981 cgcgccttcg gcggtcacca ccgtcagtac ctgccgccgc gtcagcccga ggtgggcaag
     2041 caaaccgaat tccttgcggc gcgccagcac ctgtgccgag aagctggccg cgatgccgaa
     2101 cagcccgatc gcgatggcga cgccctgcag ccagtaggtg acggcaaagc ttcggtcgaa
     2161 gatgcgcagc gagatcgcac ggatctcgcc gggcgacgcg aactcgagca actcgccgtc
     2221 catgccgtgc cggcctgcca tcgcgcgcag cttctgttgc accgcactcg ggtcggtgcc
     2281 cggtcgcagc caaagcgcga ggtcgttgat gcgcacgtcg ccggtcagcg tctgccagtc
     2341 gtccgaagcg atcgcgaccg ccccgtgctg ccgcgcatag tcgcgccaca cgccgcgcac
     2401 gaacacgcgg gctcgctgcc cggtcggcag tggcagcatc agccgcgttc ctggtgaggc
     2461 accgtacagc gccaccatcg cttcgctgac gtaagcggcg gtttcgcccg cgggcatggc
     2521 gaccagatcg cccaccagcg gcagcgagcg cgctggatcg gccagcggcc gcgcgatcag
     2581 cgccaccgcc ggctgggccg gatcgatctg caccgatgag acccgcagcg tctcgacgcg
     2641 aatcacacca tcgacacgcg ccgcgtcgcg cacccagccc ggtgccagcg tgatcacgtc
     2701 gctgccgccg gtcgaggcac cggcacgcgc atacaggtcg gcgggcagca cggtgtcgag
     2761 ccaggctgtg atcgagtcgc ggaagctcgc gaccatcacc gtcagcgcga ccgagagcgc
     2821 gaggctcgcg accacgccgg ccaccgcgac ggtggcagtg ctgcgcaggt ggcgcgcgcg
     2881 ctccacggcc agcagcgcca gcgcctgctt tggcggagca atcgcgcgca gcagcaaccc
     2941 gacgccgagc ggcacgcaga cgatgccgcc gagcagcagc agggccaccg acaggtaggc
     3001 ggcgagtggc atgtcgaaga tcggcggcaa cagcagcaga gccagcgccg ccgcgatcaa
     3061 cgccgggccg aaccaggcca cggcttcgag atcactggcg tccccgagcc ccttcagcgc
     3121 ctgcgccggt gccagccgct gtgctgcgcg cgccggcagc cagccgccaa cgagcgcagc
     3181 gacgacgccg agcccgccat acagcagcgc cgcagtcacg ctgaagtgca gcgtcggcgt
     3241 gacgccggga aagtagccgc cgcccaggtc gcccgccagc agctgcagtg ccgctgcggc
     3301 cagcgcggtg ccgagcgcca gcccgagcac gctgccggcc acgccgatca gcgccgactc
     3361 ggccagcacc agccgcagcc gctggcttgc agagagcccc agcacgccga gcagcgcgaa
     3421 ctgcggctgc cgctgcgcga ccgacagcga caggatcgag aacaccagga acgcgccggt
     3481 gaacagggcc accagggcca gcaccgtcag gttgacgcgg taggcgcgcg atacgttcga
     3541 gatgcgctgg gccgcttcat cgggtgatgc cgcgcgcacg ccctgcgcgg caaagtcctg
     3601 cagcgccagg gcagccacga ctttgcggcg atcggcgcct ggcttcaagc gcacgtcgat
     3661 ccggctcaat ttgcccagca tgccgaactg aacctgcgcg ccagcgatgt ccatcaccac
     3721 gagcggcggg ccgccggccg cgacactgcc gtgcacgcgc agggcaacga tgccgtccgg
     3781 cgtctgcagg cgcaacgtgt cgatccggcc gagccgctgc cgagcggtgg cgttcagggc
     3841 cacggcgtcc gggtcgaaca ggccgaggcg atcgggctcg ccttcgcttc cccggggtgc
     3901 tgacaagttc ggcagcagcg ctggcgacag cggcgccgcg accagcgcgt cgatgccgag
     3961 cacgcgcagc ggcacgcgtt tcgtcgcgcc ctgcgcatcg atcgtcagcg cctgcgtctc
     4021 gatctcgacc accgggctgg cgagcgcgat gtccgggtgc ctcgcgacgc gctcgtacag
     4081 tgtttcgtcg aagccgccgc gctggccgcg cagctcgaag tcgggctggc cgttgatcga
     4141 tcgcaccgct gcgctgaact cgctgagcgc cgactggttg atcagctgca ccgagaaggc
     4201 aagcgccacg cccagggtca ccgccagcag cgccgccgcc tggcgccacg ggtggtgccg
     4261 ccactcgggc caggtcagat gcttcagcag cgcgagcatg cggtcagcct gaaggcgcgc
     4321 gttcggcttc cacgatcccc gcaggggtca gcgtcagcac ccggtctgcg cgcgccgctg
     4381 ctgcgcgcga atgcgtgacc agcacgcacg ccgcgccttg ttggcgcacc tgcgccgaca
     4441 gcaggtccat caccttgtcg gcagtggccg ggtcgaggtt gccggtcggc tcgtcggcca
     4501 ggatcagctg cggtcgatgc accagcgcgc gtgcgatcgc gacgcgctga agctgcccac
     4561 ccgacagcgt ctgcggcagg cggtcggcca gcgcgtcgag gccgaccgcc tgcagcacct
     4621 cgtgcacgcg cgctgcatcg ggctggtgct gcagcagcaa gggcaggccg acgttgtgtg
     4681 cgacgctcag gtgcggcagc acgtggaagg cctggaacac gaagcccagg tgctcacggc
     4741 gaaacagcgc ctgcgccggc tcggacaggg cggtgatgtc ggtgcctgcg atgtgcaccg
     4801 agcctgcctg caccgtgtcc agcccggcga tgcagttcag cagcgtcgat ttgccgaccc
     4861 ccgattcgcc gaggatggcg atgaactcgc cgcgcgccac ctgcaggtcg acgttcgaga
     4921 acaccggctc ggtgccgtag cgcttggaca gggaacggac atcgagcagc ggcatgcgcg
     4981 ggtggagcct tggcgaatcg gatgcctgcg caacgggccg gcggcgcgat tgtcggcgct
     5041 tgcatggcgc cgggttcctg ccttgcgcaa tggccctgcg gtgacgcctt gtagcgatct
     5101 ggcgatcgat cccgcggcgc gccgcgctac agtgatctgc atggttcccg aatcgtcttt
     5161 gcggcgggct tttccgctga tctgtgctgc cctgtggctg gccggttgcg gcacggcacc
     5221 cagcccgagc gcgccttcga agggaacggt cgtacccagg ccgacgctgg cccaggaacg
     5281 ccagcgcatc ggcgatctgt tccagggcac gccggtcaca ctggcgatgg acggcgacag
     5341 cagcctgcgc gccgaggtgc cgctgaagtt cagcttcgac cccggccgcg ccgtcgtcaa
     5401 gccgccgttg gctgcgctgc tcgaccggct ggccggcagc ccggccacac gcggaggcag
     5461 ctgggtggtc gccgcacccg gagatccgaa gaacaagggc aactcgctgg cgctggaacg
     5521 cgccgccagc acgcgcgact acctggtcgg tcgcggcgcc gatccgctgc ggttctcggt
     5581 ctcggcgctc ccgggagtcg gcgaggccgc gctgatccgc atcgtcgtga aggccccgcc
     5641 ctcggcaagc cgctgacgcg ggacccgagt cgacacgtcg tccggatcag cccgccggcc
     5701 gggctggcaa tgtgtcggcc agccgcagcg ccagtgccac gacctgagcc tgtgcgtccg
     5761 ctgcatcaca cggcaccaca tggcgctgcg gcatgctgcg caaccaggtc agctggcgct
     5821 tggccagctg gcgcgtggcg gcgattccgc gctcgggcag gtcgctcagg tcgtcggcct
     5881 gcagcgcttc ccaggtctgc cggtagccga cgcagcgcat cgatggcagg tcggcatgca
     5941 gatcgcctcg ttccatcagg gcctgcactt cgtcgacgaa gcccgcctcg agcatctgcc
     6001 cgaagcgggc ttcgatgcgc tggtgcagcc acgcgcgatc gccgggctcg agggagatca
     6061 gtggcggcgt ctcgaccggg gtcttgtcct ggtggaacga cgacagtggc tgcccgctga
     6121 cccggtgcac ctcgagcgcc cgctggatgc gctgccgatc gcccggtgcc agccgcgcgg
     6181 cggtgaccgg gtcgacgcgc tgcagctccc ggtacagcgc atgcaggccc tggcgttcgc
     6241 actgttcgtc gagcgccgcg cgcaccgcac ggtcggccgc cggcatcgcg tccagcccct
     6301 cgaacagcgc cttgaagtac agcatcgttc caccaacgag cagcggcaga tgaccgcgcg
     6361 ccctgatctc gccgatcagc cgctgtgcat cgccgacgaa gcgcgcggcc gagtaggctt
     6421 cggacggatc gatcaggtcg atcaggtgat gcggtaccgc agcgagctcg gccgcgctcg
     6481 gcttcgcggt gccgatgtcc atgccgcggt agaccagcgc cgaatcgacg ctgatgatct
     6541 cgaccggcaa cgaaggttgc cgctcggcga acgcgcgtgc gatcgcgagc gcggcagcgg
     6601 tcttgccgga ggcggtggga cccgccaggc acaggatgcc gcggttggcg accggcaacg
     6661 agcgggcggt ggtgggtgcg tcaggggtcg aggtcacggg atcacttcag aagggaaggg
     6721 ggagtggccg ggccgtgggc cgcggttcgg gcatcacgac gcggcagcac ccggaatgcg
     6781 ccatgacgcg acagcgcgat gctggcggcg gctgtcgacc gtagcgaacg agcgtgctca
     6841 gttccaccgc ggctgggtca ctgcgacgat gagaacgagc aggcccgcga acaagccggg
     6901 cacggcaacc agcgccagca cggcgatggc cgcaccggtc ctgccgctgc ggcgcagcaa
     6961 gtgaccggtc cacagcacca ctcccagacc accgagcagc aacagccaca ggccgaggtt
     7021 gaacgacgat accgagccgt cggccatgcc gatcaggaag tacgtgacgg cgatgacggt
     7081 gcaagccagc gcgcagcaga agaacgtgcg gtaggcgagc gtgccgcgcg tggcggcagg
     7141 gagatccgat gtcgtcatgg tgagtggttg gcgccaaacc gggttgcggg acgatggagg
     7201 ctacctgcct gctgcccggg ttgcgacgaa cgacccgcac gggatcggcc cgctcacccg
     7261 aacacctcat ccgcacgcag ataccgccac tgcccgaccg gcaggtcgcc cagcatcacg
     7321 cgaccgatgc gcacccgctt gaggccgatg accttcaggc cgacggcttc gcacatgcgc
     7381 cggatctggc gcttctttcc ctcctgaagc acgaaccgca gctggtcgtc gttcagccat
     7441 tcgacgatcg ccggcttcag cgcctcgccg tcgagttcga ggccgtgccg cagcagctcc
     7501 aggtcgtccg gtgacagttc actcgatgcc ggcccttgac cgagcggccc ggccacgcgc
     7561 accaggtatt ccttctcgat ctcgctgtcc tcgccgatca actgcttggc gatgcggccg
     7621 tcctgcgtca gcaccagcag gccgatcgaa tcgatgtcga gccggccggc aggaaccagg
     7681 ctgcgcaggt gctcgcgcag gaagcgcgtg ccggactggt cgtcggtcca ctggttctcg
     7741 ggcttcacca gcacgaccgc cggctcgtag ccgtcttccg cctgaccact gacgtagccg
     7801 atcggcttgt tgacgatcac ggtgaccttc tgcgcctgct ggaacttggc gcgtgcatcg
     7861 acggtgatgc gctgctcggg cagcacgcgc gagcccagtt ccttcaccac ttctccgtcg
     7921 acgcggaccc agcccttctc gatccactcg tcggcctcgc ggcgcgagga cagtccgagc
     7981 tcgctcatgc gcttcgacag acgcaggctg ccgggtgctg cttcgggctg ctggtggaac
     8041 gggccacgtt cttcatcgtc cggatgacgc tcggcgcgtt cggcctggcg ccatttcggc
     8101 tcgggcggtc gctgtggcgg ccgctcgaac gggcgctcca ggggtcgtgc cgaggggcgc
     8161 tctggcggcc gttccggcgg gctgaccggg aagcgcggct gcgcaatgat gggcggtcga
     8221 tcccgctgct gcgcttcctg cccgcgcgct ggctccgggc gacgttgagc tctctcctga
     8281 ggcgggcgtc cagcctgcga gccggcgaag cgttgtcgct ctgccgtgcc ttcgcgatcg
     8341 cgggtgctcg ggcggggcgg cgggccgtcg cgtcggtcct ggcggggctc accacgcggc
     8401 tcgttgcgcg ggtccctgcg aggttcagcc gaccgttccg gcgggcgaga ggcgcgcggc
     8461 gggcgcggcg catcccgcgg atcggccgcc ggccccgaat gcgattgaaa cggcttgcgg
     8521 gccggcggcg cacgttcggg ccgatcgaac ggtggtgccg cgtcgcgtcg cgcgcgatcg
     8581 gcctgcgctt cggccagcgt cggacgtcgg cgcaccccca tgccgcttcc gcgcaccgga
     8641 gttcgctcga tgcgcgccgc cggggcagcg gggttcttgg gcttgatctt cagcgtggcc
     8701 atgcgggtcc agtcggtgtg cggggtgtgc agtgaaaagc ggggcgcctc tcggcgttga
     8761 tcggggcttc agcggccacg ccagaacagc gcgtcgagtt cgcgccgcgt gatctgccgc
     8821 cacgtcgggc ggccgtggtt gcactggtcg gcgcgctcgg tgcgctccat gtcgcgcagc
     8881 agagcgttca tctcgtccag tgtcagctgg cggttggccc gcacggcgcc gtggcaggcc
     8941 atggtcgaca ggatctcgtg ctgcgcgcgc tgcaccacgc tgctggcatc gtgctcgccc
     9001 agttcggaca gcacgctgcg cgcgagttcg accacgtcgc cgccagccag tgcggccggc
     9061 tgcgatcgca ccgcgaggac ggtgggcgac agcggcgaga cgtcgaggcc gagggagcgc
     9121 agcgtgtcgc cgtgcgcttc ggcggcagcg atctcggcgg tggtcgcggc gaaggtggcc
     9181 gggatcagca gcggctgagc ctcgatgcgc gaagcagcca ggcttgcctt caagcgttcg
     9241 tagaccacgc gttcgtgcgc cgcgtgcatg tcgacgatca ccagtccctg cgcgttctcg
     9301 gccagcacgt agatgccggc gatctgcgcg atggcctcac cgagcggtcg cgcggattcg
     9361 tcgtgggtgg ctgctttggc caaatggacg agcgacaagc ctgctcgggt tccatcgtgt
     9421 ccgggagcgc cagcactgcc ccacggcgcg gcgggctcgt tcccgaacag caccgtggcc
     9481 tgatgcaggc tcaacgcgac ctgcgcgctg gtcggtgtgc cgccatagcg gtcgttgcgg
     9541 gcgtcgctac gtccccaggc gacggggccc gtcggatcag gggccgcagc cagctgaccg
     9601 aatgtgccag gcagcatctc gaccgcttcg gtccgcgtgg tggcttctac accggcgatc
     9661 gcctctgcca gcgacgcctc gaccgcccgc cgaaccgcct ggtgcacctc gcgcgattcg
     9721 cggaagcgca cctcgatctt ggtcgggtgc acgttcacgt cgacgcggtc cggcgcgatg
     9781 tcgatgaaca gcacataggc cggctgccga ccgccgtgca gcacgtcttc gtaggcgctg
     9841 cgcacaccgt gcgcgatgag cttgtcgcgc acgaagcggc cgttgacgta gacgtattgc
     9901 tggtcggcgc ggctgcgagc cgatgccggc gtgccggcgc gtccggtgac acgcagcacc
     9961 ccgaggtcga gcgccacggc gcggctgtcg gcgatgaact cggtgcccag cacgtcgcgc
    10021 aaccgggcct cggcctcggc acgccgccat tgctgcacca gcctgccttc gtgccacacc
    10081 gcgaacgaga cgtcggggcg ggtcagcgca tgtcgcctca ctgcctcgac acaatgcgac
    10141 agctcggtcg attcggtttt caggaacttg cggcgtgccg gcgtgctgaa gaacagttca
    10201 cgcacctcga cgctggtgcc gatccctcgc gcggccggcg tgatctcgcc ggagcgcgcg
    10261 tcgagccttc tggcctttgg atcgtcggcg catcggctgg cgatcgacac ctcggccacc
    10321 gacgcgatcg cggccagcgc ctcgccccgg aagcccatcg tcgacacgtt ttccagatcg
    10381 ctcagcgatt cgatcttgct ggtcgcgtgg cggcgcaggg ccagaggaag ctctgccgcc
    10441 ggcagcccct gtccgtcgtc ctcgaccagc acagaacgga ttccaccacc tgtcagcttg
    10501 acggtgatct cgctcgcgcc ggcgtcgagg gcgttgtcca ccagttcgcg caccaccgat
    10561 gccggccgtt cgaccacctc gccggcagcg atctggctga tcagttcatc gggcagttcc
    10621 cggatcgggc ggcgcggtgt gaggggccca cgggcattca tcgacgcatt gtagaaaccg
    10681 cctgccggcg cggcggggtc tgcggtcggc aaggcgaaag gggcggtcac cgagccccct
    10741 ccataatttg ccgcatggaa atcgtctcgg tgctgatcga cttcatcctg cacgtcgacg
    10801 tgcacctcgc gaatttcgtg cagacctacg gtgcctgggt ctacgcgctg ctgttcgcca
    10861 tcatcttcgt cgagacaggc gtcgtggtga tgccgttcct gccgggtgat tcgctgttgt
    10921 tcgtcgttgg cacgctgtgc ggcgcggggc tgatgagcct gccgcttgcg atcgcggtgc
    10981 tgctggtcgc ggccatcctc ggcgaccaga ccaattacag catcggccgc cacttcgggc
    11041 ccaaggtgtt ccagtgggag caatcgcgct tcttcaacaa ggcggcgttc gagcaggcgc
    11101 atgcgttcta cgagaaatac ggcggtatca ccgtcgtgct ggcgcggttc atgccgttcg
    11161 tgcgaacctt cgcgccgttc gtggccggag tggccgcgat gtcgcgcacc accttcacgc
    11221 tctacaacgt ggtcggcgcg ctgatctggg tggtcggcct gacggtggct ggttacgtct
    11281 tcggcaacct gccgttcgtc aagcagcatc tggacaagat catctgggcg ctgatcctgg
    11341 tgccggggtt gatcgcgatc tggggggcgc gcaaggcgcg ctcgaaagtc acgctgccag
    11401 cgtcgggctg acgcggtaga gcagatttcc agcaccttat ttcgttgggg ctctgccaac
    11461 ggcttgctct gcaacccatg cgccgtaacg tgggatggtt tcttggcgtg actgagggat
    11521 ccaggccatc ccaatgaaaa gggcgacccg aaagtcgccc tgcatccatc cgaaagatgg
    11581 gttggtcgtc agacggtctt tctgcgacgg ctcacgccca atgcagcgag agccagaccc
    11641 acgagcgcga ggcttgcggg ttccggaact tcagcggatt tggcaatgcc gaactcaccc
    11701 acggcgaacc cgccactgtc cccggaagcg gtgatcgtca gtgatgtcaa ttccgtgccg
    11761 tccgttccgt agataccgaa cccaaacgca ccggcattcc cgttcacaga ttcggtgctg
    11821 gtgctgccat cctgtgccgt caatgtgaag tcgaacaaat cgaaatcgtt ggactcgacg
    11881 tacaaataga aggcgctgac atcgttcggc agggtgatag tcagcgattg cgataagttg
    11941 aagtacacat ctccggcgta cccgtggctc cacgtggccc atccgttgcc gacggcccgg
    12001 gtgttgactg agttgctgaa gcccagattt ccgcccagtg gcgacgccaa cgacaggatg
    12061 tcggtgaagc tgccaatccc atcatctccg aagggggtca tggtgtagcc acccagcgtc
    12121 gctcccgggg cgcccgtgcc gagttcgctg attaccactc ccgccgttgc gtgaccaagt
    12181 gctgcagtca atacgatcgc gcaagccgcc ttgatcagta agggtgaacg ggccgtcgag
    12241 ggttggcatg gtgcggtgac tgctggattc atgaaacggc cttgtaagaa tggttggggc
    12301 ttgatattgc tgattagcca tattagttaa gcaaaattca agccgaatat tcaactcttt
    12361 gattcatatg gacttgtcag tatctatcct gctgacctgt aaaagatttc gacattttgt
    12421 tgggcggccg cgggcagcac gagcggtcga tttgcagctt ggcagatggc cagagcggcc
    12481 caagtcgtgc gcatgaccca attggcctgc ggcagccgga gctcgtgcct gcggtttggt
    12541 tttcaggttc ttcgcgcccg cgccagcggc ggattcttcg cgaagtaccg cttgatcccg
    12601 accgtcagcg cttcgaccag ctgggcctgg tagcgcacat cccgcagctt ggcctcttcc
    12661 tccggattcg agatgaacgc cgtctcgacc aggatcgacg ggatgtcggg ggccttcagc
    12721 accgcaaagc ctgcctgctc gacgcgcgcc ttgtgcaggc gcccgactcg gccgatctgg
    12781 ccgagtacct cgccgccgag ccgaaggctg tccttgatct gcgcggtggt gctcatgtcg
    12841 agcagggcgt gcatcaccgt cgagtccttc gacttgacgt tgatgccgcc cacggcgtcg
    12901 gccaggttct cgcgattggc catccagcgc gccgcggcgc tgctggcgcc gccctggctc
    12961 aatgcgaaga ccgacgcgcc acgggcctcg ggcgtgaaga acgcgtcggc gtgaatcgac
    13021 acgaacaggt cggcctgcac ccgccgcgcc ttgcgcacgc gatccttcag cggcacgaag
    13081 aagtcggcgt cccgcgtcag catcgcgcgc atccccggca gggcattcag gcggttgcgc
    13141 aattgcagcg cgaccgacag caccacgtcc ttttcccgca ggccgctcgg tccgacggct
    13201 cccgggtctt caccgccatg gccggggtcg agcgcgacga tcacgagtcg gctgatgcga
    13261 ctcgcagccg aggccggcga ttcgggcggt cgcacgctgg gcccgtcggg agggttcgcc
    13321 ggcgtcatgc ccagcggctt gccggccggg gtcggcgcgc cttttggcgg tgcgctggcg
    13381 ccgtccggtg gaatcgccgt gccgagtggt ttgtcgacct gtccgatgaa ttcgccgagt
    13441 gcatcgcgca ccgattgcgc agcctgctcc tcggcgcgtt ccttctcctg gatgagcgcc
    13501 agcagcggat cgcgttcctg caccgggtac aagtcgaaga ccagccggtg cttgtaggca
    13561 gccacggggg tcagcgcgaa gagctgcggc ttgatcgcct gcttcaggtc gatcaccagc
    13621 cgcactacgc gcggctggta ctgcccgacg cgcacgcctg cgatgaacgg atcgtctgcg
    13681 cgcacctgtc ccaccagatc gcgcagctga ggactcaggt cgaggccatc gacatcgatg
    13741 acgagccggg tcgggctttc ggccaggaaa tgccgcgccg ccagcgcgct gtcggattcg
    13801 atcgtgaccc gggtgtagtc gtctgccggc cacacgcgca ccgcgacgat ggcagccgca
    13861 tgcgtgccgg acacgccgac cagcgtcagc gagacagcgc ccatggttga cagcgcgcgg
    13921 cgcctggccc gcgagggcgg ggcgcctgat gcgctttccg gtgccgtcat ggcatcagcg
    13981 tggcgccgag cggcgtgccg gcccggaagg tggcccggcg tcgcgtgtcg tcggcatcgg
    14041 ggtcgctcgc aggcgcatca accatgtcga tgtcgatgcg caggtcgcaa ggtggcagca
    14101 ggcctgcagc gcgctcgggc cactcgatca gcttcaggcc cgggccggcg aacacatccc
    14161 ggaagcctgc atcctcgaac tcctgtggat cgtcgaagcg atagaagtcg aagtgccaga
    14221 tgtcgagccc gggcagcgca tacggctcca ccacggcgta ggtcgggctc ttgacgtggc
    14281 cactcacgcc gagtgctcgc agcaggtgcc tgacgaacgt cgtcttgccc gcgcccagcg
    14341 tgccgtgcag ttcgatgacg gcgttgcgca atccggcgtg gcgtgccagt tcgcgcgcag
    14401 tggcagcgca ggcggcctcg tcgggccaca gcgcggtgca ggcttctaag atcggcatgc
    14461 cgtcagaggg cgtcgccgag tcggcaggaa aggtggaggt gcatggtcgc tgggcacggg
    14521 ttggatgagt cgctgctgtg gcctcgcgtg caggcctggg cgggtgagct tggattttcc
    14581 cagatcggtg tggccgacgt ggacctgagc agcgccgaac ccggtctgca ggcttggttg
    14641 gacgccggct tccatggttc catggattac atgcagcgac acggcctcaa gcgtgcccgt
    14701 ccgcgagaac tggtgcccgg caccgtgcgc gtgctgaccg cgagaatgga ctacctgcct
    14761 gctgaatcgg atccgcccgg cccgccaggc gattggcaga ccgtcgaatg gcagcgactg
    14821 caggcccctg aacgggcgac ggtgtcgatg tacgcgaggg gccgcgacta ccacaaggtg
    14881 ttgcgcaatc gcctgcaggc actggccgat cgcatcgccg ctgcggttgg cccgttaggc
    14941 caccgcgtgt tcaccgattc ggcgcctgtg ctcgaggtcg agctcgccgc gcgcagcgcg
    15001 ctgggctggc gcggcaagca cacgctggcc ttgagccgcg acgcgggttc gatgttcttc
    15061 ctcggggaaa tctacatgga cctggcgttg ccggtgaccg gtccggtggc tgcccattgc
    15121 ggcagctgca gcgcctgcgt cgacgtgtgt ccgacgcggg ccatcgtggc gccttaccgg
    15181 ctcgatgcac ggcgttgcat cagttacctg acgatcgagc acgccgggcc gattccgatc
    15241 gagttccggg aagcgatggg caaccggatc tatggttgcg acgactgcca gctggtatgt
    15301 ccgtggaaca agtacgcgca gcgcagcacg ctggccgatt tcgatgtgcg cgctgtgctc
    15361 gccgacccga cgctgctcga cctgtggcgc tggagcgagg ccgatttcct gcgccacacc
    15421 gaaggcagcc cgattcgccg tatcggacac gagcgctggc ggcgcaatct ggccgtggcg
    15481 atgggcaatg cagtgcgggc gttgcccgaa tcggagctgt gctcggcgct tcgcggggca
    15541 ctggcgcagg cgttgcccga cgccagcgaa ctggtgcgcg agcacatcga atgggccttg
    15601 cttcaatcgg cagcgaaggc tacagacgag ccccgatcgc cagccacacc ggcacgctga
    15661 ccatgccgag cagtgtcgac atcgtgacca gcccggccac gaacggaccg tggccgccca
    15721 tgcgcatggc cagcacgtag gcgctggagg cggtcggcaa ggccgcgaac gccagcacga
    15781 cagcctgctg caggggcggc aggcccaccc agatcgacag tgcgagcgcg atcgccggca
    15841 gggccacatg ccggatggtc aggaaagccg ctgccagcgc cggtgcggcc tgcaggccgc
    15901 cgagcttcag gccggcaccc acggccatca ggccgagcgg gatcgcggcc agacccagtc
    15961 ggtgcagcgt ggtccccacg gcatcgggca ggtgcagccc ggcgaggttg aagagcagcc
    16021 cggcgagcgt gccgaggatg aacgggttgc gcacgagttc gcggacgtag gcatgaccgc
    16081 cgtggcgcgc caggggccac accgcaccga tgttgcagat cggaacgcac agcgcgatca
    16141 gcaacgccat cagcgcgagc gtttcggccc cgccgagtcg ctcggcgagc gccagcgcga
    16201 tgtaggagtt gaaacggaac gcagtctgcg cgcccgaggc gtgcagccgg gcgtcgacgc
    16261 ccggccatcg gcgaatcgca tacgccagcc cgatgccgca cagcacggtg cccacgccga
    16321 ctcccgcgag agcagccgtc tgcgccgggt gcagcgggct cttcgcgatc gagctgaaca
    16381 gcagcaccgg gaacagcagg aagtaggtca ggcgttcgac cgcgtcccag accgggcggt
    16441 caagcagggt gtgacgacac agcagaaacc cgaccacgat gagcagcagg tcgggaacca
    16501 gcaagagggc atgcgacatg gctctcgagt gtgccagcca caatggccgg atgagttcgc
    16561 tacgggacag cacggccgcg atcgaccgct tcatcgatgc gctgtggatc gaggatgggc
    16621 tgtcggccaa cacgctggcc gcttaccggc gtgacctgac gctttacgcg cactggctgg
    16681 ccgccgggcc cgatgtcgcg ctcagcggca caaccgaaac ccatctgcgt gattacgccg
    16741 tcgcgcgcca tgaaggcagc aaggccagca gcacgaatcg caggctgacg gtgttcaagc
    16801 gctatttccg atgggcgctg cgcgagcgcc tgatcgacgc cgatccgacg ctcaagctgc
    16861 tggccgcgaa gcagccgccg cgcctgccga aggtgctcag cgaagcgcag gtcgaggcac
    16921 ttctggcggc tccggacgtg accacgccgc tcggccttcg cgaccgcacg atgctggaac
    16981 tgatgtatgc cagcggcctg cgtgtcagtg aactggtcac gctgaagacg gtgcacctgg
    17041 gcctggtcga tggcgcgctg cgcatcacgg gcaagggcgc caaggaacgg ctggtgccat
    17101 tcggcgaaga agcacgtgcc tggctggagc gctacctcgc cgaggcgaga gccgacatcc
    17161 tgaagtcgca ggcgagcgac gcgctgttcg tcaccgcgcg cggcgggccg atgacgcgcc
    17221 agatgttctg gcggctgatc aagcagcacg ccttgcgcgg cggggtgcat gtgccgctgt
    17281 cgccacacac gctgcgtcat gccttcgcca cgcatctgct gaatcatggc gccgacctgc
    17341 gcgcggtgca gatgttgctt ggtcactcgg acatctcgac gacgacgatc tacacccacg
    17401 tggcgcgcga gcggctgaag acgctgcatg cggcgcatca tccgcggggt tgaacgcacg
    17461 ggccggcagt caccgaggtc gcgatcccga ctcctacaat ccgccgctaa tcgaaagggc
    17521 cttcccagca tgaaagtcac cgcgtccaac ttcaaggcct acgacattcg tggcatcgtc
    17581 ggccagacca tcgatgaagc cttcgccgag caccttggcc gggccttcgg cagcgaggcc
    17641 gtcaaagccg gcgagcgtgc cgtggccgtg ggtcgcgacg gacggctctc cggtccgggc
    17701 ctcgccgcag cgctgatccg cggcctgatg tccaccgggc tcgatgtcgt cgacctcggc
    17761 gcgatcacga ccccgatgct ctactacgtg gccgccacgc gcggtcggcg gggctgccac
    17821 agcgggatca tggtgacagg cagccacaac ccgaagaact acaacggctt caagatggtg
    17881 ctggccggcc gtgcgatcta cggcgacgac atccagaacc tgcgcgtgcg catggaagcc
    17941 gaggactatg tgacgaaggc cggtcgctcg gccccgatgg acatcggccc cgagtacagc
    18001 gcacgcatca ccggcgactg caagctgaag cgccgcatga agatcgtcgt cgattcgggc
    18061 aacggcatcc cgggcgcctc ggcacccggc atcctgcgcg cactgggctg tgacgtgacc
    18121 gagctgtact cgaaggtcga tggcgacttc ccgaaccatc acccggaccc gagcaagccc
    18181 gagaacctgg ccgatctgat ccgcacggtg cagaccaccg atgccgagat cggactggcc
    18241 ttcgacggcg acggcgaccg gctgggcgtg gtcaccaagg acggcaacat catctatccc
    18301 gaccggcagt tgatgctgct tgcgaaggac gtgctcagcc gcaatccggg cggcacggtg
    18361 atcttcgatg tcaagtgcac gcagcgcctc gcgccggtga tccgcaaggc cggtggcaag
    18421 ccgctgatgt ggaagaccgg tcactcgctg gtcaaggcca agatgaagca ggtcaaggcg
    18481 ccgatcgccg gcgagatgag cggccacatc ttcttcggcg agcgctggta cggcttcgac
    18541 gacgccactt acaccgcggc gcggatgctc gagatcctca gccgcagcaa ggacccgagc
    18601 aaggtgctga acgcgctgcc aaccagctac agcacgccgg agctgaatgt gccctgcgcc
    18661 gagggcgagc cgaagctgct ggtgcagaag ctgctggaaa ccgcgaagtt tcccggcgcg
    18721 ctggagatca tcacgatcga tggcctgcgc gccgaataca aggacggatt cgggctgatc
    18781 cgctcgtcga acacgacgcc cgttctggtg atgcggttcg agggtcacac cgagaaggcg
    18841 ttgcatcgga tcgaggccgc attcatggcg gccttgaggg tggtcaagcc cgacgcgcag
    18901 atcgcggccg cggcgcactg aacccgtctt gcaggtcatg ctgcagcgtc gggccgcccg
    18961 ctgatcgtgc agcgcctggc gcgggtcgcc tactccacgc tgctgcggct ggcgctgcca
    19021 gcctacgtgc tgaagctgtg gctgcgcggg cgggcggagc cgctgtaccg gtttgccatc
    19081 ggggagcgct tcgggtggta tcgcgtcgat cggcgcagtg atccgccctc atcgggctgg
    19141 gtctgggtgc acgcggtatc gctcggcgag acgcgggcag cctctgcgct ggtcaatgcg
    19201 ctgcgtgatc tgcgccccgg catgcgtctg ctgctgactc acggcactgc gactggccgc
    19261 acagccggtg ccgccctgct gcgcccgggt gatcgccagg cctggctgcc gttcgacacg
    19321 ccgggttcgg tggcgcgttt cctggcccat ttccagcccg ccatcggtgt cctgatggaa
    19381 accgagatct ggcccaacct gctgctggag gcaagccgcc ggggcatccc gacggtgctc
    19441 gcgaacgcgc ggctcagcga gcgcagtgca cgcaaggcca ggcgcttcga ggcgctgttc
    19501 cggccggtgg ccgagagcgt gccgctggtt ctggcgcaga cgcaggacga tgccgatcgc
    19561 ctgcgcgccg cgggcgcgcg tcgcgtcgag gtctgcggca acctgaagtt cgacgtgacg
    19621 cccgacgcgg cacaactcgc tcgcggatcg gcctggaagc tcgcctgcgg tcgccgcatc
    19681 gtgctggcgg ccagcacccg cgaaggcgag gagggcccgt tgctggcggg ctggaaccag
    19741 cagcccgcac cgcgaccgct gctgttgatc gtgccgcgcc atccgcagcg cttcgacgag
    19801 gtggccgcgc tcgtcgagag cagcgggctg acgtgctggc gacgcagtct ctgggccgat
    19861 gcgccgccag ccgacgccgg gacggccgac gtctggctcg gagactcgat cggcgagatg
    19921 gctgtctatt tcagttgtgc ccgggtcgcg ttgctgggcg gcagcttcga gccgctcgga
    19981 ggccagaacc tgatcgaggc cgccgcgtgc ggctgcccgg tgctgatggg gccccacacc
    20041 ttcaacttca gccaggcagc cgatctggcc ctgcacgaag gggctgcaca gcgggtggcc
    20101 gatatcgacg aggcggtgca ggtggccgtg gggatggcgt ccgagcctgc gggcgctgca
    20161 ggccgatcac cggatgtccg tcccagtccc cagcgcgccc tcgaattcgc ggcgcggcat
    20221 cgaggcgccg ccgagcggat ggcccggcag atcagcgcgc tgctcggctg agcttcgccg
    20281 gcgtcccggc caccgtgcct cagggaccca gcagccggct caccgcggcc acgtcgtcgg
    20341 gtttcagctg gccggcagcc tggcgcaggc gcaagccgcc gagcagcgtg tcatagcggg
    20401 ccttggacag gtctcggcgg gtggtgaaga gctgcgtctg cgcgttcagc acgtcgatgt
    20461 tgacccgcac gccgacgcgg tagccgagct gggtggcctc gagggcgagg gcgctcgatg
    20521 attcggcggc ctcgagcgcc agcacctgtg cctgcagcga ctgcacgccg aagtaggcct
    20581 ggcgagtgcc ctgcgacacc gcacgccgcg ccgcctcgag atcgttgcgg gacttgtcct
    20641 cgagcaccag cgtttccttg acgcggttct ggaccgacag cccggcgaac agcggcaggt
    20701 tcagctcgag gccgatgctg gcgctcttgg tgctgccgct gcgcgacagc tggctgcccc
    20761 cgctgccgcc cgagctgtac gaatcgctga ggctgcccac cacgtccacg gtcggcaggt
    20821 ggccggcctg ggccttgcgg gtttcgagct gggcgacttc cagcgacagc tgtgccagcc
    20881 gcaccgacgg atgctgcacg tcggcctgac cggcccaggc ttcggcatcg gcgggcgaca
    20941 acggcggcag cgtcaccggg ggcttgagcc ccagcggctc gacgttgctg cgaccgacca
    21001 gctgatcgag cgcgatgcgc ttgttgcgca ggtcgttgtc tgcggcgatt tcctgcgcgg
    21061 tggccaggtc gtagcgggcc tgcgcctcgc gggtgtcggt gatggtggcg gtgcctacct
    21121 cgaaattacg cttcgccgac gccagttgtt cgttgatcgc gaccttgctg gtgcgcgtgg
    21181 tggccagggc gtccttggcg gcgagcacat cgaaataggc ctgtgcgacg cgcaccatga
    21241 ggtcctgctc ggcggtttcc agccgcaggc tggcggcgtc gagttcgcgc tgtgcctggt
    21301 cgatgctggc gctgctggcg cggttgaaca gcggttgccg accggccatc tgcagggccc
    21361 ggttgttgaa cgatgccgtg cccagcgcgg tgtcgacccg gccgatgttg gcgttcgcgc
    21421 cgagattcag gctcggtcgc agcagcgcgt ctgcctgtgc cgcgcggtac tgcgccgact
    21481 cggccagcga ccgggcggac aggtaggtgg cgtcgtagtc gtgcgcggcc ttgtagagct
    21541 cgtcgaggct ttgcgcctgc gccgcgcaca gcccggccgc gaacagcaac ccggtgcaga
    21601 cgcgcttcac tcggagtccg ccacgagagg ccagcccgcc atgtacagcg attgccatga
    21661 gatgtccttc ggaccggcag cgcggatcat cagtacctct gcaccgacgc atccacctcg
    21721 gtcgaccatg catcgatgcc cccggtgaaa ttggtgaccg atgcgaagcc ctggcgctgt
    21781 tccaggtacg ccgcgacctg cgcgctgcgc acgccgtggt ggcacaggca gatggtgggt
    21841 cgcgcaggat cgagttcgcc gatgcgctgg atgatctcgc tcatcggcag gttcagggtg
    21901 gtcaggcctt ctgcgcgcag cgcggcgcgc tcgaactccc acggctcccg cacgtccagc
    21961 agcagcggca ggtcggtgct cgaagcctct gccagcaagg ccgcgaactc gcgggcgccg
    22021 atgtgctgca tcagaactgg aagcggctcg gctcgtcgaa gcccgtgagg cgcggtgcca
    22081 cggtgtcgaa caggtcgacg ctggcgacgg cgccgtcggt ggggctgccg gcgctgcggg
    22141 tgtaaagcac cgcccgcatg atcggaagct gcccgacgac cgcgaccagg cggccaccgg
    22201 gcttcaactg atcgaacagc gacgccggca cctgcggcac cgaaccggac agcacgatca
    22261 cgtcgaaagg cgcttcgccc gggcagccct tcgagccgtc agcgtgccgc acggtgacat
    22321 tcgagctgcc cgcccggcgc aggttgtcgg cggcaaagac cgccagttcg gcatcgatct
    22381 ccaggctcag cacctgttgc gcctggtggc ccagcagcgc ggccatgaag cccgatccag
    22441 cgccgacttc cagaacgcgt tcgtgcttgc ccacggccag ttcctgcagc agtcgcgcct
    22501 cgacccgggg cgccagcatc gattgcgccg gtccctgcgc agtgtgcaac gggatctccg
    22561 tgtcgacgaa ggccatcgag cggtaggccg ccggcacgaa gtcttctctg cgaaccgctg
    22621 ccagcagggc cagcacgtcg gcgtcgagca catcccacgg acggatctgc tgctcgatca
    22681 tgttgaagcg ggcgagttct gtgtccatgg aaatcggatc tttcttgatt gaggggttca
    22741 ttcagccagc aacggacgtc gtcgatggtt tgccgcgcat tttatttccc ctggatcagg
    22801 gggacgggga cggcctgctg cgtgctgcgc aggaacctgc gcttggccca cgccgacaga
    22861 tcatccaggt agcagtagac caccggcacg accaccaggg tcagaagcga cgatgcgatg
    22921 acgccgccga tcaccgcctg gcccatcggc gcacgctgct cggaaccctc ggacagcgcg
    22981 aaggccagcg gcaccatgcc gaagatcatc gccagcgtgg tcatcaggat cgggcgcagg
    23041 cgcacacggg cggcgagcag cagggcttcc tcgcgaccga ggtggcctgc tgcagtcgcc
    23101 ggctcgcccg gcacctcgcc cgcgcgagcg cggatcgcga aatcgatgag caggatcgcg
    23161 ttcttggtca ccagccccat cagcatcacg atgccgatga tgctgaacat gttcagggtc
    23221 gagcggaaca tcatcagcgt cagcacgacg ccgatcaggg tcagcggcag cgaactcatc
    23281 agcgcgatcg gctgcaggaa gctgcggaac tggctcgcca ggatcatgta gatgaacacc
    23341 accgccaagc ccagggcgcc gaccgcgtag ttgaacgatt cggtcatgtt cttggtgctg
    23401 ccgccgaagg agtatcggta gcccggaggc caggcgatgc cgtcgaggat ccggcggatg
    23461 tcggccgaca catcgccaga actgcgtccg taggcgttgg catccatgtt gatctcgcgg
    23521 ttcaggtcgc gccggttgat ctggttcgga ccggtcgagg ggctgacgtc cgcgacctgc
    23581 gacaacctga cgatgcgcgg catgccgtcg gcgccggcca ctaccggtac cggcaggcgc
    23641 tccaggtcgg ccatcgagtt gcggctctcc gggttcagcc tgacgatcac atcgtagttc
    23701 tcgccgtcgg gtgcccgcca gttccccacc gtcgtgccgg cgaccagcga gcgcagcgag
    23761 gtcgccagcg aattgacatt gagtccgagg tccgaagccg cttcccgcct gacattgatc
    23821 gccacggtcg gcttgtcggg cttcagcgtg ctgtccaggt cgacgagtcc cggcacccga
    23881 tgcaactgct cggtgatgac cttcgacagg cgctccagtt cgccgaggtc gctgccctgg
    23941 atcgagaact ggaggctctt gcccccgccg aggtccgggg tgccgatgtt ggtcatcgtg
    24001 acgccgggga tgcgcgcgag gcgctcgcgc atcgggacgg tcatctcgtc caggctgcgc
    24061 tggcgctcgg cgcgatcgac cagtcgcacg taggtgctgc cgaagatctt gccggccgcg
    24121 ttgccgctgt tgatcgtggt cacggtgtac cggacctcgg gcaactcgcg cagcgccgca
    24181 tcgacctgac gagcgcgcgc ttcggtcatc tcgagcgacg agcccaccgg cgtgtagaaa
    24241 ttgatcgtcg tctcggaaaa atcggccttc ggaacgaatt cggcgccgag cagcgggatc
    24301 agcatgaagc tgccaccgaa ggtgcccatc gcgatggcga gcgtggacag gcggtggcgc
    24361 agcgaccagc gcaggatcgc ctggtagccg ttgcccagcc gcacggtgaa gcgctcgaac
    24421 tgctccgtga cacgtccgag cgtgcggtcg tagaggctgc tcgtgccgcg tggcgcaccg
    24481 ggcagatgag acgcctgcgg gtcgtgccag atgctcgaga gcatcgggtc gagcgtgaag
    24541 ctgacgaaca tcgagatcag caccgccgcg acgatggtga ggccgaactc gtggaagaac
    24601 ttgccgacga tgccgcccat gaaaccgatc ggcaggaaca ccgcgacgat ggacagcgtg
    24661 gtggccatca ccgcgaggcc gatctcctgc gtgccgtcga gtgccgcctg gtgggcggac
    24721 ttgcccatct gcacatgtcg cacgatgttc tcgcgcacga cgatcgcgtc gtcgatcagc
    24781 aggcccacgc acaggctcag cgccatcatc gtgatgctgt tgatcgtgaa gccgaagaca
    24841 tacatgaagc cgaaggtccc gatcagtgcg atcggcagcg tgaggccggt gatgacggtg
    24901 ctgcgccagg agttgaggaa caggaagacg atcagcaccg tgagcgccgc accctcgatc
    24961 agcgtgcgct tgacgttcgc gaccgcgacg cggatcgccc gcgagttgtc gcggttgacc
    25021 tcgacgacga cacccggcgg cgtcaccgat tcggcgcctg caagcgcctt gcgcaggccg
    25081 tcgaccacct cgatggtgtt ctcgccctgc gacttcagca ccgacagcag caccgtgcgc
    25141 tgcccgttgt agagggccag gctttccact tcctgcgggc cgtcgaccac gtcggcgacc
    25201 tgccacagct tgaccggcga gccgccgctc accgtggcgt tgccggtgcc cggcgtggcc
    25261 gcgccacggc gcgcaacgac gatctcgcgg aagtcctccg ggcgcttcag gcgcgcgttg
    25321 acctgcacca cgcgctcctg ctcggccgag cggatggcgc ccatcggcag ttcctggttc
    25381 tcgctgcgca ccgcgttgat gacgttgtcg acgctgacgc ccatcgcctc catcgccgcc
    25441 ggcttcaggt acaggttgat ctgccgcgac acgccgccca cgacgctgac cgagccgacg
    25501 cctcgcacgt tctcgagccg cttctgcagc acctgcgtgg cccaggtcgt cagttcctgc
    25561 ggcgactgct tgcccgacgg cgacaggacc gcgatattga agatcggcgt gctggccggg
    25621 tcgaagcgcg agacgcgcgg ctccttgacc tcgtcacgca ggctggggcg caccagcgcg
    25681 atcttctcgc gcacgtcctc ggcggccttg cgtccgtcga tgttcaggtt gaactcgatg
    25741 atgacgatcg agttgccctc gtaggagcgt gaatacagcg agctgatgcc tgcgaccgtg
    25801 ttgaccgctt cctcgacctt cttggtgact tcggtctcga cgatctcggg agaggcgccg
    25861 gggtagtcga actggaccac caccgtcggg aagtcgatgt tcgggaactg gtcgaccgac
    25921 agcctctggt agctgaacag cccgagcacc acgaaggcca gcatgaccat cgtggccatg
    25981 accggattgc cgatcgagac tcgggtgaac cacatggtcg gcgtcgggcg gctctagcgc
    26041 gcgtcgactg gggccgacgc ggcggtggcg gggctgccgc tggcgccccc gcccgcaatg
    26101 cggaccagcg agccgtcgcg aacggcgccg gccgatccgc gcagcagcac ggtgccctcg
    26161 gtgaccccgg tggtcagctc gaccgccgct tcgacgctgc cgtcgaccag caattcgccg
    26221 cgctggccga gcgtgacctt gcgctgctcg atgcgatcgc ccaccacagc cagcacgtag
    26281 ggctgggcct gatcgacccg caccgccgac accggcaccg ccagcacgtt gctgctgcgc
    26341 aggtcgatgc tgccggtggc gaacagaccc tggcgcaggc ccggctgcgg gtcgacggcc
    26401 aggtagacca tgaccgcgcg cgtgccggcc tgcgtgctcg ggttgatgcg aaccaccctg
    26461 gccgtcgccg gcgcggcgag cccatcgatc gatagccgcg ccgtctgtcc gaccacgacg
    26521 ccgcccacgt cttccggcgt gacggccgcc tcgagctcga tgcgcgacag gtcgaccacc
    26581 tcgaccagct tggcatcgat cgcgacccgc tcccccggct gcaccaggcg ttgcgacacc
    26641 aggccggata tcggggccac cagccgggtg tcggtgcgcg acttgcgcgc gagattcaat
    26701 gcagcgagtg ccgcctcgtg ggtggcgcgt gcggcggcct cgttcgatat cgaggtctcg
    26761 aggccggtgg tcgagatgaa gccctgcgcc accagagcgc ggttgttgtt cagcgcacgc
    26821 tgggcgatgt cgagctgggc ccgtgccgat gcagcattct ggtcggcctg ctggacccgc
    26881 agatcgacct ccagtgtgtc gagctggccg atcgtctgcc cggccttcac ggtgtcgccc
    26941 tcgcgcaccg tcagcgactt gacctcggca gcgaccttgg cacgcaccac tgcgctgtcc
    27001 acggccttca gtccgccggt gatcgccagc gtgcgctgga aggtctgggt gcgcgccacc
    27061 acgaggtcgc cggccccgag ctcgagcgcc ggcggcggct tggccggggc ggctgccagg
    27121 gcccgctcct gcgcgcggcc atacagcgtg cgtccgatga acccggccac cagcagcacc
    27181 gccaggatca gaacgaccca tcccaagctg atgcgcttca tcttcgtgtg tacttcatgg
    27241 aactcgtggg acggcgtgca acaggatggt cgcgcagttc accgccgagc agtatcagcg
    27301 gattgcagcg cgcctgctcg acgacgggat tccatccggg gcgggaggcg gcgttcgtgc
    27361 ggtgggcgcc ttgtcgcggt agccggcgca ctctggagcg tgttggaggg tgttcgggca
    27421 tcgatggagg gcacgaccgg gcgcgatgtg cagcccgcgg gcattatcgg tgcgccgtcc
    27481 accccgcctg gtcggctccg gcccgggcct cacgcacagg cagaatcgcc cggatgctct
    27541 cgctggaccg gttctcattc caacagctgt tgctggtcgc cttcctgttg atcgccggat
    27601 tgctcagcgc ggcctcgctg cgcgggctgt tcacgatcga ggcgctgatc actcagagca
    27661 gccagggcgc tcggcaggcg gtgtcgctca gcgccgccgc tcagcggctg gccgagcgca
    27721 gtgtgacgat ggagcgtgca gcgcggcagt tcctggtgct cgatgacccg gtgctgcgcc
    27781 agcgcttcga cgatggggca cgcgaggccg gcgaggcact gacccagctg gtggccggca
    27841 cggatgccgc ccctcgccgc gatgccgcag cagccggctg gcgccggacg ctgcagacgg
    27901 tgcgccagca gctcgtcggt gaacccgaca ccctgcgcga ccgcgagcag acgctgaccg
    27961 ctgcgttccg cgaactcgga cagatcaaca tcgacctcgc cgaccaggtc cgcgtggcca
    28021 ccgaggtgcg caacgcgacg ctgctcgaac ggctcgaaca gggcagggcg gcgctggcga
    28081 ggcagatcat cgtgaccatc gccgtggcgg tcgggctggc gctggcattc ggaatctggc
    28141 tggcacggcc gctgaagcgg ctggaagccg ccatcgtcgg cctgggcgag aaccgactcg
    28201 accggccgat cgagatgcgc ggtcccgccg acgtgcgaca gctcggtctg cggctcgaat
    28261 ggctgcggct tcggctggcc gagctcgacg ccgacaaggc gcgtttcctg cgccacgtgt
    28321 cccacgaact gaagacgccg ctggccgcga tgcgcgaagg cgtctcgttg atggaggatg
    28381 gcgtgaccgg tccgttgaac gagtcgcagc gagaagtcgc gcgcatcctg cgccagaaca
    28441 ccgcggtgct gcaggcgcag atcgaggacc tgctgcgctt caacacggcg gcgttcgacg
    28501 cgcgccggct ggtgcggcgg ccagtcgaac tgcgttcact gatctcggag caggtcgaga
    28561 tgcagaagct gcagtggcag gcccgccgcc tgaccgtcgc ggtgcgtggc gagacgctga
    28621 ctgtcgaggt cgatgccgag aagctcggca ccgcgctcgg caacctgctt tccaatgcga
    28681 tccgcttcag ccccatcggt ggcaccatcg tcttttcggt gtctcagtgg ccgggacggg
    28741 cgcgcatcga catcaccgac cagggcccgg gcgtggcgcc ggccgaccgt gcccgcgtct
    28801 tcgagccctt tttccgcggt gaacgccagc cccccgatgc cgcgcgaggc agcggcatcg
    28861 gcctgtcgat cgtgcaggaa ctggtgcgcg cccacgatgg tcagatcgac ctgttgatgc
    28921 ccgaggccgc agaggtcgag ggtgcccatt tccggattga attgcctcat gcccctgctc
    28981 gttgaaccct cacccgcgag tcggctccgt cggcctggcg ccggcgtgct ggttgtttcc
    29041 ctgtggtcgg ccgtgctggt ggcaggtttg tctgcctgca cgtcccttcg ctccgttgac
    29101 cggccggcat ccgacgtccc tgtggcctct gccgctgctt ctgcgccggt ggtggttgcc
    29161 gcgccgttgc ctgcagtgga cgcagcctcc gcaccggtcg ctgcgccggc ggcctcggcg
    29221 ccagtcgatg aggccacgcc cggagatctg gccgcgcgtc gcttgctggc gtatcacgat
    29281 caagtccgcg ggatgcttcc tagtgacctg gcacaggaga tcaaccgcct cgccgcgtcg
    29341 ccgcctgcgc ctgaaacctc tctggagctg gcgctggcgc tgatgcagac ccgcaacggc
    29401 ggtgaactga accgcgcgat cggcctggtc gaaccgcttg ccaggggcgg ctcggccgat
    29461 cagcgcgcgt ggcagccgtt cgcgcgcctg ctgctgtccc gcctgctcga gctgcgccgt
    29521 ctcgaagagc tgctcgacaa gcgcagccag gagctgcgcg acagccagcg cgaggtgcgg
    29581 cagctcaacg agaagctcga ggcgctgaag gccatcgagc gcagcctggc gccccgtccc
    29641 tcgtctgcgc tgccgccctc gaccctgtcc ggcacagcct ctttcgctcc gacgcgccgc
    29701 acgccgtgat gcccgccaac ctgctcgtcg tcgatgacga ccccgacctg ctgcagttgc
    29761 tgtcgatgcg cctgacggcc gcgggctacc gcgtcaccgg tgtcggttcg gcggaagcgg
    29821 ccctggcaca tctcgaagcc gtccggccgc agctggtggt cagcgacgtg cagctgccgg
    29881 gccgcgacgg gctggcgctg ttcgacgaga tccgagcccg ccatcctgcc ctgccggtga
    29941 tcctgctgac agcgcacgga acgattcccg atgcggtcga ggccaccgcg cgcggcgtgt
    30001 tcacctacct gaccaagccc ttcgatggca aggcgctgct cgacaagatc gcgcaggcgc
    30061 tggcgctcgg cgcgccggaa gcccacaggg aggggcccga cgaagcctgg cgcgcgccca
    30121 tcgtcagccg ctcggcggtc atggcggagg tgctcgccga agccaagatg gtcgctgcgt
    30181 ccgacgccag cgtgctgatc tgcggcgaaa gcggcagcgg caaggaactg ctcgcgcagg
    30241 cgatccatcg cgcgagcccg cgtgctggca agcccttcgt cgcggtcaat tgcagcgcga
    30301 ttcccgaagc gctgctcgaa tctgaactgt tcggccacgt caagggctcg ttcaccggcg
    30361 cggtggcgaa tcaccgcggg ctgttccagg cggccgacgg cggcacgctg ttcctcgacg
    30421 agatcggcga catgccgccg gccctgcagg tcaagctgct gcgggtgctg caggagcgtt
    30481 cggttcgtcc ggttggagcg aaccagtcga tcccgatcga cgtgcgcatc ctctcggcga
    30541 cgcaccgcga tctcgacgcg gcgatggccc agggccagtt ccgggaggat ctgtattacc
    30601 gcctcaatgt ggtcgctctg accttgccgg cgctgggtga ccgccgcgag gacatcccgc
    30661 tgctggccaa ccacttcctg tccaagctcg ccgagaagta cggcaagcgg ctcaacggat
    30721 tcgcgcccga ggcgctgaag gcactgacgg ccgctgcgtg gcccggcaac gtgcgccagc
    30781 tgcacaacgt cgtcgagcag gtctgtgcgc tgaccaccac gcctctcgtg ccgctcgccc
    30841 tggtgcagcg cgcgctgcgg gtgcccagcg tcgaggtgct gagcttcgcg caggcgcgcg
    30901 agcgcttcga gcgcgactac ctggtcggac tgctgaagct gaccgacggc aacgtggccg
    30961 atgccgcccg gctggccgat cgcaaccgga ccgagttcta ccggctgctg cagaagtacg
    31021 gcctgacgcc ggggttgttc cgcaacgagc cccgtcccga cggcacgctg gatcccgcgc
    31081 cgccggccac gctgtcgcca atcggcgaca agtagaagtc gtttgttttc aacgacttgg
    31141 cgtgcaccat cggctggcgt gtcgccgccg ggagacgtct gcccccctgc aggggcagta
    31201 gaaacccgcc attccgcatc cgagggccgg gaaatgcgct caagtcgttg attcgaaagg
    31261 aggttctcgc gttggcacgc acgttgcgct gaagccctca tcgcaccgat gagagcctca
    31321 tgaaccaccg tctgtcaccc cgcttgaacc gctcctggac cgaagcaggc accggctcgg
    31381 gcgccgcggc acgtcgtgat gttggcgcca gcatgccgcc gatgaaccgg ggctcgatgg
    31441 tggcgcgtcc ctggcggggc gtcatcggcg gattgatcgt cgggtggatc gcgctgatgg
    31501 atcgtctcgc gggccgccct gccgtgcctg acaccgacag ccccgcgccg ccgatgcccg
    31561 ccgatgccgg cgcgacggca gcctggaagc gatcggcggt gcgccggcgt cgcgcgctgc
    31621 tggctctggt cgcgctgtcg gtcgtcggag ccaccgcgat gctcgatcag gtgctgccga
    31681 cctacagcag cgatgcactg cgtaccgcgc agatcgcgct gttctcggtt ctgtttggct
    31741 gggtgtcggc cggtttctac accgcgatga tggggttctg ggtgcagtgg cgcggtgacc
    31801 cgcacgcgct gtcgagccgc tcggtcctca atcgtccgat cgcggccggt gcgcgtaccg
    31861 cgctcgtgat gccgatctgc aacgagcagg tctcgaccgt cttcgccgga ctgcgcgcca
    31921 cggtcgaatc gctgatcgca gccgggggat cgcgtctgtt cgatgtctat ctgctttccg
    31981 acaccagcga cccgacgatc cgcaccgccg aactggcggc ctgggccgaa ctgcgcgagg
    32041 cgatcggcag cgtgcgcatc cactaccggt ggcgtcagcg ccgcacgcag cgcaagtccg
    32101 gcaacgtggc cgacttctgc cggcgctggg gcaggaacta ccgctacatg gtggtgctcg
    32161 acgccgacag cgtgatgacc ggcgactgcc tgctgacgct ggtgcgcctg atggaagccc
    32221 atcccgacgc cggcatcgtc cagacggcac cgcaggccag cggcctgtcg acgatccatg
    32281 cgcgggcgca gcagtttgcc ggccgcgtga ccgggcgcct gttcagtgcc ggcatgcagt
    32341 tctggcagct cggcgagtcg cattactggg gtcacaacgc gatccttcgc gtcgaaccct
    32401 tcatgaagca ttgcgggctg gcgccgttgg ccggccgggg cgggctgagc ggccacatca
    32461 tgtcgcacga cttcgtcgag gctgcactga tgcggcgtgc cggctatcac gtgtggctgg
    32521 tgcacgacct ggccggcagc tacgaacagc agccgcccaa tctgctggaa gagttgcagc
    32581 gggaccgccg ctggtgccag ggcaacctgc agaacgcgcg gctgatcgcc gagccgggcc
    32641 tgcacggcgt gcaccgcgcg atgctgctga ccggcgcatt ggcctacctg tcggcgccgc
    32701 tctggctggg ctacgtgctg cttggtgtcg cgctgtggct gttcggtggc cacccgtcct
    32761 tcagcggcga tgcggcggtg ccgttcgaga tgctgatgct gtgggccggc acgggggtca
    32821 tgctggtgat gccgcgcgcg atgggggtcg tcacgatcct gatgcgccgg gaacagtcgc
    32881 agtacggtgg cggcgcagca ctgatccgcg gcgcgctgat ggagggtctg ctgtcggtgc
    32941 tgcaggctcc gctgaggatg gtggcgcaca cgctgttcgt ggtcgtcgca ctgacgggac
    33001 tgaagctgga ctggaaatcg cctccgcgcg aggccgccga catcggctgg gccgacgcat
    33061 ccaaccgctt cgcgttgctc ggcgccgcga tgctggcgct ggccggcgcc gcactggcgc
    33121 tcgaacctgc gacgctgctg tggctggccc cggtgggcgt gccgctgctg ctggcggtgc
    33181 ccctggcggt gctgaccagc cgcgccggac tcggcgagcg ggtgcgctcg ctcgggctgc
    33241 tggtcacgcc cgaggaaacg cgggcgccga ccgtgctgcg cgaggcggcc aactacgcct
    33301 tccagaacgc gcgcaatcgc agcatcaagc tcgattggcg cgatgcgatc gacaacccct
    33361 ggttgttcgg cgtggtgcgc gcggcgatgg ggtcacgcaa caccatgtgg ggccaccgtg
    33421 gccgtgcgcg ccgccaactg gtggggcgcc tgcgcgccac gcacgacatc gacagcctga
    33481 gcaccgccga tcgcatgcgc ctgctcagcg agccgcaaca cctggtgcgc ttgcgcgatc
    33541 agctgatggc ccgcacagat ccgtcgacag catcctgggg cagggtgggc tgacgggtcc
    33601 gaacctgacg cagcgttccc ggacgcaacg cgtctgcccg gcgatggggg cggggtttcc
    33661 gcagcgcctc gtgcgatctg gcgagatgcg gcaaatgctg cggcagggcg ggggccggat
    33721 cagcacgagc cgtcaaccct gccccgtatg atccttcggg tcttctcacg gatcccacct
    33781 tggacattcc cctgctgatc aaagccgccc tgatgggcat cgtcgagggg ctcaccgagt
    33841 tcctgcccat ctccagcacc gggcacctga tcctcgccgg ctcgctgctc gacttcaccg
    33901 gcgagacggc caaggtgttc gagatcgcga tccagaccgg tgcgatgttc gccgtgatct
    33961 ggcagtaccg cgaacggctg cagcgcaccg tcggcgggct gggcagcgac ccggtcgcgc
    34021 ggcgcttcgc gatcaacgtg ctgatcgcct tcattccggc ggttgttctc ggcctggcct
    34081 tcggcaaggc ggtcaaggcg catctgttcc acccggtgcc ggtcgcgatg gccttcatcg
    34141 tcggcggctt cgtgatcctg tgggtcgaga gccgccacaa gcgcctgtac ggtcagctcg
    34201 atctgcaggg cggccgccgc gctcgcgtcg agaccgtcga cgacatgggc ccgctcgatg
    34261 cactgaaggt cggcttgctg cagtgcctgg cgctgatccc tggcaccagc cgctcgggat
    34321 caaccatcat cggagcgatg gtgcttggct tctcccgcaa gtgcgccacc gaattcagct
    34381 tcttccttgg cattccgacg ctgatgggag ccggtgccta ctcgctgtgg aagcagcgcg
    34441 aggcgctgca cgcgggcgat ctgccgatgt tcgcggtcgg cctggtgttc gcgttcttca
    34501 gtgcactgct gtgcatccgc tggctgatcc gtttcgtgtc gacacacgac ttcgtggcct
    34561 tcgcctggta ccgcatcgtc ttcggcggca tcgtgctgct gacggcctgg caaggctggg
    34621 tcaactgggc cgactgacgc ccctccaacg caggccagat cagtcgcggg cgggcagctc
    34681 gatcactgcc ggtggatcga catccacctc gtcgcagttg tctcccgggc cctcccggtc
    34741 cagcaccagg aagtcgctgg tctcgcggag tgccagcagc gggtggtgcc acacgccggc
    34801 ggcgtaattg acaccctggt gaggctgagc gatgaagcag cgcagctgcc gggcggtcgg
    34861 cgctggaccg cgtggagcaa cgaccaccag gtaggtccga actgctcgat ctgcggagct
    34921 tccaccgaac aacggcatga aagcctgcga gccgtgcggg tggcgctcca tcacggccac
    34981 ggtgaacggc agcgagcgcg gtgcgccgcg aaagatgctc gcgatgactc gcccgtctgc
    35041 accgggatcg aggtgggcca ggtcgtgaaa gcgctcggtg ttgccggcgt tgatcgtcca
    35101 gtggcgcgcg gcctcgccgg cctcgagcac ctcgccgaac ggtgcgaacg cttcggcagt
    35161 cagcggttcc acccgcagcg agctcgcagc gcgcgtcatg aacccagcgc ccgcgccagc
    35221 ccggtcacag cagcacgatc ctgccgtgca atcgcaaccg actcactccg ccgtcgggaa
    35281 agatgttgaa ccgcacgtgg gtcaccgggc cgagcgcggc cagcgtgtcg aagcggtgct
    35341 cggcatccat gccgagcttc tgcggggcca gcagttcggg ccagaacatg ctctgcgtga
    35401 tcagcgacga ctcggtggcg tggcccggca cgttcgcggc ctgcagcgag cacgagtcgg
    35461 ggtagttgcc cttgaaatgc gcggtgtcga cctcgacccg ctcgatcacg ccgtgtgccg
    35521 ccagcgcgac gatgcaccag tcgttgcccg gctcgcggcg gcggcgtgtc tcccagccgt
    35581 ctcccatgtc gacgccgcgt cccggcagca gcagcgccga tgcctgcccg aaatgctggt
    35641 tgttggccgc gaccacccag gcgccgttga tcgcggcagc gagatccagc gctccgcccg
    35701 acgccacggc atcgacctcg ggacgtccgt agatgcgcag ccgtgccacg ccgccatccg
    35761 gatagatcgc caggcgcaca tgcgtccaca cctcgtggtt gtcgatcgca tggtggtgat
    35821 gggcgttgcc tgcgagtgca accgtgggaa gcagcggtgc ccagtgcgcg gtgccgtccg
    35881 ggacctcatg ggtgctgcga cagacttcga gcgatgcggc gggtgggtag ttgccggtga
    35941 agtggcgggt gtcgatgtcg accccgaaga tgcggccggg tcgcgccagt cgcaccacgc
    36001 accagtcgtg accaggcccg cgcttgcgtc gtgtttccca gccgtccatc catttgccgt
    36061 ggtcgtcgta cttgccggca acgaacaccg gatcggtggg tgcgagcaga cgttcgcgcg
    36121 gcgcgaagaa ctcgtcgctg cacgacacga cctgcgcacc cagccgcgga tcggcgaggt
    36181 tggtgcagcg tcgcgtgaat tcgggggcgt tcgggtcgat gatgggattg gccatggtcg
    36241 ggacgcagct caaaacgtcc attctgcggc cgttgctgcg ggatgattca atccgtgcag
    36301 tccaccgaca gacacgcccc cgggaaaccc atgagcctcc acgccgatcc cccctatccg
    36361 cgagacctga aaggccacgg tcccgatgtg ccccatgcac gctggccggg cggcgcgcgc
    36421 atcgcggtgc agttcgtcct caactacgag gaagggggcg agaactcccc gctgcacggc
    36481 gacgcgacct ccgaggtctt cctgtcggag atcatcggcg cgcagccgtt cgagaaccgg
    36541 cacatgagca tggagtcgat gtacgagtac ggctcgcgtg ctggcgtgtg gcgcatcctg
    36601 cgcgagttcg atgcacgcgg cctgccgctg acggtgttcg gtgtcggaat ggccttgcag
    36661 cgctaccccg agctggtggc agccttcatg tcgcgcggtc atgagatcgc caaccacggc
    36721 tggcgatgga tcagtcacca gcacatgccc gaggcgaccg agcgcgagca gatcgcgatg
    36781 ggcacccagg tgctgaagca gctgaccggc ggtgtctggc cgcagggctg gtacaccggc
    36841 cgcgacagcc cgcagacgcg tcggttggtc gtcgagcatg gcggctacga atacgacagc
    36901 gaccactacg gagacgacct gccgttctgg atgccggtca catgcgccga tggaacggtg
    36961 ctgccccggc tcgtcgtgcc ctacgcgctg gacaccaacg acatgaggtt cgcgcaggcg
    37021 caggggttca ataccgccga ccacttcttc tcctatctgc gcgatgcgtt cgatgcgctg
    37081 tacgccgagg gggatcctga cgggctcgac cggccgaaga tgatgagcgt gggcatgcac
    37141 tgtcgcctgc tgggcaagcc ggggcgcatc ggggcgttgc agcggtttct ggaccatgtg
    37201 ctggcgcacg gggcggtgtg ggttgctcgc cgtatcgacg tggcgaggca ttgggctcgc
    37261 gtgcaccctt gttcctcggg gtgagaccgc gccgggtttc ggcccggctg tcgagtaact
    37321 ttcttctgct gccccagaag aaagtcacca aagaagaggg gctgaacacc agccgatttg
    37381 attggctcgc ttcggctgca cacctacttc aaaggctctg gctcgagaac atcctccgag
    37441 ctaccgcctc ggtttcctcg atcacattca gtcaactcgg tatcgatacg gcttgaccct
    37501 gcgggtcatc gacgcgagga cccacagggt cgagccgaaa cgagaactag cggctcggca
    37561 ttagcgaggt aaccgtgacc gtagtccggt gaccgttctc gtgccagagc ctctgccgtc
    37621 agccttctgg ccgcagcgtg tcggccaaat cggctggtgt tcgaggccct ttgctttggt
    37681 gactttcatt tgggcccgca aatgaaaacg aagtttcggc gaagccaaag ttactcgggt
    37741 gccgggccga aacccggcgc agtccaccgc atgatcacac ccccgccagg gcaacacctc
    37801 caacaaggtt caaagaagaa cgaatgaaac cttccgacat cgtcgacatg accgtcctcg
    37861 ccgccctctg gggcgcgtcg ttcatgttga tgcgctttgc ctcgcccgcc ttcggcccga
    37921 tcccgctggc cgccctgcgg gtcatcgggg cctcgctgtt cctgctgccc ctgctgttga
    37981 tgcggggcca ggccggtgaa ttgcgcaagc actggaggcc gctgctggtc gtcggcatca
    38041 ccaactcgac gctgccgttc atctgcttca gctacgccgc gctgtcgatc accgccggca
    38101 tgtccgcgat cttcaacgcg gcaacgccgc tgttcggcgc ggtgatcgcg tggtggtggc
    38161 tgcgcgacgg gctgaccagg cagcgggtgg ccggactcgc gatcgggttt tccggcgtgc
    38221 tgtggatggc ctgggaccag gccagtttca agccgggcgg caccggatgg gcggtggtgg
    38281 tctgtctggc ggcgccgctg ctgtacggcc tgtccgccag ctacaccaag cgtttcctga
    38341 ccggcgtgcc gtcgctcacc gtcgccgcgg gcagcctgct ggcggcggcg ctgctgctgt
    38401 gcgtgccggc ggtcgtctgg tggcccgccg tggccccgtc aatcgccgac tggggcgctg
    38461 cggtgttcct cgcgctggcc tgcacggcgc tggcctacgt gctgttcttc cggctgatcg
    38521 cgaatgtcgg accggcccgc acgatcacgg tgacgttcct gatcccggca ttcgcgctgc
    38581 tcttcggctg gctcttgctt gacgaggcgc tgacgccccg gatgttcggc ggttgcgcgg
    38641 tgatcctgat cggcaccggc ttcgcgaccg gcctgctgac gcttcgggcg cgacgctgat
    38701 cggcagcgtc gcgcctgttg cgtcagcgcg ctgcttcgcc ggaggcggtg gctgccacgg
    38761 gcgtcggcgc ggtccatcca ccgcccagcg cgcggtacac cgcgaccagc gacgtcgcgt
    38821 tcgaggtgtc ggcctgcgcc agcgcgtcgc gcgtcgacag cagttcacgg tcggcgtcta
    38881 gcacggcgag gaaatcggtg acccccgcct catagcgcag ccgggccagc cgcgacgctt
    38941 ccgatgcgct gcgttcggcg cgggccaggc tctcgcggcg ctgcgcgttg cggctgaatt
    39001 gcgcaagcga tccctcggtc tcctcgagtg cagtggccag cgtctgttcg tagttcgcga
    39061 ggctctgcag cgttctcgct tcgctggccg cgatccgcga ccggatgcgc ccgaagtcga
    39121 gcacggtcca ggtgatgccg gcgccgaccg agtagcgccg cgactggctg tcggtcaggt
    39181 cgccaagtcg ggtcgacgcc agaccgagca ggccgctgag gctgatgctg gggtacagct
    39241 cgccctcggt cacgccgacc tcggcggtgg ctgcggccag ttgccgttcg gcagcggcca
    39301 catcgggccg ccgcgccatc cattgctgcg gcgtgcccag cggcagggct gcgaggtcgg
    39361 tgaccggcag gccgggcagc ggggccggtg tggcgaggcg ggcgcgcaac tcggccggcg
    39421 gctgcgcgct cagggtcgcg aggcggtaga tcccgaggtc gatcgctgcc agcaagctgg
    39481 gcagcgcggc ctcggtgctg gcggtcaatg tcagcgcgcg ttccaggtcg agttgcgtgc
    39541 cgcgtccggc gtcgacccgg gcctgcgcga tggccagcga ctcgcgctgg ttcgctagcg
    39601 aggcctgcgt gaccgcatgc cgcagctgca gcccgcgcac ttccaggtag ttgcgcgcca
    39661 cctcggctgc gacgctggtc tgcactgcac ccaggctggc ctcgctggcg cctgcctgcg
    39721 ctgcggccga ctccttggcg cgccgctcga cgccgaagaa atccagctcc cagttggcga
    39781 cgaaggcagc cgagaaattc gcgcccgtgc gctggctgcg gctggactgg aacaccgacg
    39841 acggcggcga gatcgaccga ttggcgttgg cctcgacgcc gatgctgggc aaggtcgact
    39901 tgtcgacttc ttcgcgcaac gcacgtgact cctgcagtcg cgcctgtgcg atgcgaatgt
    39961 caccgttggc ctgcagcgca cgctcgacga gcgcactcag ttcggcatca ttgaacccgc
    40021 gccagaaggc cgcgatgtcg gcggcgactg gctcggtgtt gagcttgctg acgccggcgg
    40081 cgatgaagtt cgcgtccagc ttcgggggcg gcgcctggta gtcacgaccg acgagtgcgc
    40141 agcccgaggc cagcagcgtc agcgacaggg cagctgctgt ggtgatcctg aaagcgggtt
    40201 cacgcatgac tgtctcctgc tgcgggcaac gccgctgctg cgtctggcgc atgcgccagc
    40261 gtcgtcggca cccggccggc atggtgcagc ctggccggcg agagcacgcg cagcaccgcg
    40321 tagaagactg gcgtcagcac gagtccgaac accgtgacgc cgatcattcc gctcatcacc
    40381 gcgatgccca tggcctggcg cacttcggcg ccggcgccgc tcgacaggac cagcggaatg
    40441 acgccggcga tgaaggcgat cgaggtcatc aggatcgggc gcagccgcag gcggcaggac
    40501 tcgaccacgg cctgcaccgg gctgcgcccc tgcaactcga gctcgcgtgc gaactcgacg
    40561 atcaggatcg cgttcttgca cgccagcccg accagcacga cgaaggcgat ctgcgtgaag
    40621 atgttgttgt cggccggttg cccgaagaat ccgctgagcc agacgccggt gactgcgctc
    40681 aggatgcaca tcggcacgat caggatgatg gccagcggca gcgtccagct ctcataggtg
    40741 gctgccagta ccaggaacac cagcagcacg cacagcggga agatgtagac catcgtgttg
    40801 ccggcgatct tttcctggta gaccagttcg gtccattcat agcccatgcc gcgcggcagc
    40861 gtttccttca ggatcttctc catctcggcc tgggcctcgc ccgaactgaa gcccggcttg
    40921 ggcgcgccgt tgaagtcggc ggtgtacagg ctgttgtagc gtccgacggc ggcggggccg
    40981 aaggtgggct cgacggtcat catcgcaccc agcggcacca tctcgccggc gttgttgcgg
    41041 atcttcaggt tcgcgatgtc ctcggccttc gcgcggtagc tggcatcggc ctggacgatc
    41101 acctggtagg tcttgccgaa gcggttgaag tcgttgacgt acagcgagcc caggtaagtc
    41161 tgcatcgtgt cgtagatgtt gttgaggtcg acgcccatct gcttggcctt ggtgcggtcg
    41221 acgttggcga acagctgcgg cacgttgatc tggtaattgc tgaaggcttg cgtgatggtg
    41281 ctgtccgggt tgccgtagac gcgccccatc agctgctgca cgacgccgtt gagcgccgct
    41341 tcgccggcac cttcgcggtc ctgcagctgc agcttgaagc cgccggcgtt gcccaggccg
    41401 ctgaccggcg gcgggctcag cacgaacacg aaggcacctt cgatgccgcc caggcgctgg
    41461 ttgacctggc cgacgatcgc gttggcgctg gtctccgggg acgtgcgctt ctcgaagtcg
    41521 tcgagcgtga cgaacacgat gccggaattc gaggccgcag tgaacccgtt gatgctcaga
    41581 ccggggaatg cgaccgagct ctctaccccc gggatcgcca gcgcgatgtc gctcatctcg
    41641 cggatgatct tggtcgtgcg ctccaggctg gcgccttcgg gcagttgcgc gatgccgatc
    41701 aggtactgct tgtcctgctg gggcacgaag ccaggcggca ccttggtgaa catgaacacc
    41761 gtcaggccga gcagcacggc gtagatcagc agggcgaatg acttgcgcct gacgacggtc
    41821 gtggtcacca cgccgctgta gcggtcggcg tttcgatgga atacccggtt gaaccagcgg
    41881 aagaagcgcc cgaacaccgc atcgatgccg cgcgtcaccg cgtccttcgg ggcatcgtgc
    41941 gggcgcagca ggatggcgct gagtgcgggc gagagcgtca gcgaattgaa cgccgagatc
    42001 accgtcgaga tcgcgatggt caccgcgaac tgcttgtaga actggcccga caggccgctg
    42061 acgaacgcca gcggcacgaa caccgcgcac agcaccaggc cgatcgcgat gatcggtccc
    42121 gagacttcct gcattgcctt gacgcttgcg tcgtgcgggc tgaggccggc ctcgatgttg
    42181 cgctcgacgt tctcgaccac gacgatcgcg tcgtcgacca cgatgccgat cgccagcacc
    42241 aggccgaaca gggtcagcgt gttgatcgag aagccgagca gctgcagcac cgcgaaggtg
    42301 ccgacgatcg acaccggcac cgccagcagc ggtatcaggg aggcgcgcca ggtctgcagg
    42361 aacaccacga cgacgatcac caccagcagg atggcctcga tcagcgtcga gatgaccttc
    42421 tcgatcgagg tctgcacgaa gcgcgtcggg tcgtagacga tcgagtagct gactccggcg
    42481 gggaaatcgg ccttcagccg ctccatcgtc gcacgcacgt cggccgacag ctgcagcgca
    42541 ttcgaattgg gggcctggaa gatcgcgatc gccgaggctt ccttgttgtc aagcagcgag
    42601 cccagcgcat aggtgttcgg gcccatctcg acgcggccga ggtccttgat gcggatcagc
    42661 gagcggtcga tcgggtcggc cttgacgatc acgtcggcga actgctgctc ggtggtcagc
    42721 cgcccctgcg tgttgatcga cagctggaac tcggtgccgg ccggcgaggg cgatgcgccg
    42781 accacgccgg cggcgacctg ggcgttctgt tcgcgcagcg cggcgacgac gtcgcccgag
    42841 gtcaggctgc gtgccgcgag cttctgcgga tcgagccaca cgcgcatggc gtagtcgccc
    42901 gagccgaagg tctgcaccga acccatgccg gggatgcgca gcagctggtc gcgcacgttc
    42961 agtgccgcgt agttgcgcag gtacagcgcg tcgtatcggt tgtccggcga gaccatgtgc
    43021 acgaccatcg tcaggttcgg cgagctcttc tcggtggtga cgccaagctg gcgcacgatc
    43081 tcgggcaagc gcggcagggc tcgctgcacg cggttctgca ccgcggtttc ggcggattcg
    43141 gggttggtgc cgatcttgaa gctgatggtc agcgtcatcg ctccgtcggc ggtggccagg
    43201 ctgttcatgt agagcaggcc ttcgacgccg ttgagctgct cctcgagcgg tgtcgccacc
    43261 gtctcggcaa tcacccgcgc attggcgccc gggaactgcg cgcgcacgac cacctggggc
    43321 ggggccacct cggggtactc ggagatcggc agcgtgaaga tcgagatcgc gccgatcagc
    43381 gtgatgacga ccgacagcac cgccgcgaag atcggccggt cgatgaagaa gcgggagatg
    43441 ttcatgtgcg cctgctcagg actgtgccgc cgacgcggcc gaagccggag ctgcaggcgc
    43501 ggcgccgggg gcgcctggtt gggcaggagc tggcggcagc ggcatgccgc gctcgtcggt
    43561 ctcgaccttg atgggggtca ccggcgcgcc gggccgcacc cgctgcagac cgtcgacgac
    43621 caccagttca cccgctttca ggcccgacgt gatcacgcgc atgccgtcga tcagcgtgcc
    43681 cggatggacc tcgcgcgcct gtgcgacgtt gccttcgccg atcacgaaga ccagcttgcg
    43741 gctctggtcg gtgccgatcg cccggtcggg cgtcatgacc gccgggtagg ccgcgccgcc
    43801 gacgagttgc agtcgggcga acagcccggg cgtgaactgg cccctggcgt tgtcgaacac
    43861 gccgcgcatg cggatcgaac cggtctgcgg gttcagctgg ttgtcgacga agtcgatctt
    43921 tccttcatgc ggaaagccgg cttcgtcggc cagccccatg cgcaccagct gctgccctcc
    43981 ggcacccttg cgcagccgaa ggaaggtctg ctcgctgccg tcgaagtagg cgtagaccgt
    44041 ctgcgtggcg acgagcgtgg tcagcacgct ctggtcgttg acgaggttgc cctcggtgat
    44101 gatggcccgc gagagctgcc cggccatcgg cgcgcgcacg ctggtgtagc cgaggttgag
    44161 ttgcgcgaca cggaccgccg cctcggcggc tgcgatgtcg gcgctgctgg tcgccgcacc
    44221 ggaagtgagc tggtcgaatt cctgtgccga caccgccttg gcgtcgagca gcttcttcgc
    44281 gcgggccagt tccgacgtcg ccagtgcggc gcgcgagcgg gccgcgctca gctgcgactg
    44341 cgcacgtgcc agttcggcct ggaacggctt cgggtcgatg gtgaacagca tcgcgccctg
    44401 ggcgacgcgg gcgccatcac ggaaatgcac cttgtcgatc gtgccgccga tgcgcggccg
    44461 cacctcgacc gaacggggtg cctcgagccg gccggtgaac tcctcgatgt cggtgacggt
    44521 gcgctgcacg gctgcagcca cggacaccgg gggcgctccg ggtggcgccc ctgccggggc
    44581 accctgcttc gagcagccgc cgatcagcag gatcgcgatc aacgcggagc tcagggacac
    44641 ggaaagcgcg gggcgcgagg cggcgatgtt catggaactc ttgcacggag agatccgctc
    44701 gaacgcgcgg ttcgatcgaa tcggggttgt cggatcgaga gcccgaactg gccggtgggc
    44761 gcagccgaga ccggggtcac ggggtcccgc cggataccga tcgatagcgt cgatcgggcc
    44821 tcggtgagaa cccgtagacg ataaccaaac tcgggccgct ctgcatttcg gcggcccggg
    44881 gcccgaggcg atgtcagcaa cggcggaaca ccagatccca gacgccgtgc ccgagcttca
    44941 atccgcggtt ctcgaacttg gtcagcggtc ggtagtcggg ccgcggcgcg tagccgtcgg
    45001 cggtgttttt caaggagcgc tcggcgagca gcacctccag catctgctcg gcgtagggct
    45061 gccagtcggt cgcgcaatgc aggtagccgc cgggtgacag gcggctggcc agcaacgaga
    45121 cgaagggggg ctggatgagc ctgcgcttgt tgtgcttggt cttgtgccac gggtcgggga
    45181 agaacacgtg cacgccggcc agtgatgcgg gcgcaaccat gtgttcgagc acctcgaccg
    45241 cgtcgtgccg cacgatgcgc acgttggtca gcccgcgctc gccgatcagc ttgagcagcg
    45301 cgccgacgcc cggcgcgtgc acctcgacgc cgaggaagtc ggtgtccggc agcgcggccg
    45361 cgatcgctgc cgtggcgtca cccatgccga acccgatctc gaagaccagc ggcgctgtgc
    45421 gcccgaaggt gcgtggcgca ttcagcagcc cggcggtgta gggcacggtg tagatcggtc
    45481 cccattcgtt catcgcacgc acctgtccgc tgcccatgcg gccggcgcgc agcacgaacg
    45541 acttgatcgg gcgatgcggg gcagcttccc gggtgggttc tccggggggg ctgtcgaccg
    45601 ggtcggcggg gatgttcgaa ctcatggatg ggtcggcggc aaggtcggaa atgcaaacgg
    45661 ccctgtgagg gccgttgtcg aggcaggtag cgcagccgga acgttcgagt cggccgtggc
    45721 tgcggcgccg aggatagcgc actggactct tctctctggc cgggccgtcc gggggcggag
    45781 agccgtcgcc gcagtctgga cgcctgcctt gcagccgaca ggaaatgtgc cggctagagc
    45841 gttcagtcct gcccgatgag gcaggcgtgg ctctgggcgg accgggcctg ctccatgaat
    45901 cgctcgaagc ccggagcatc gagcggtctg gcgtagaggt agccctgcgc gaagtcgcag
    45961 ccgatcttgc cgagaaggtc catctgttcg gcggtttcga cgccctcggc caccacgctc
    46021 aggcccagcg aacgagccat cgagacgatc gcctggcaca gcgccagatc ccggctgccg
    46081 tcctggatgg tgcgcgtgaa cgactggtcg atcttgagga agctggcgtc cagcttgttc
    46141 aggttggcca ggcaggagta accggtgccg aagtcgtcga tggcgaggtg catgcctgcc
    46201 gcgcgcaacg tgcgcagctt ggacgcggtt gcatggtcgg ggtgcaccat catgctttcg
    46261 gtgatttcaa tgatcacgct gtcgccggcg atgccggtgc gggccagctc ggtgacccag
    46321 ttgtccgccg agtcggcgat gccctgcaac tggctgggcg acatgttgat gctgacctgg
    46381 aagtcgcttc caaactgctg ctgccagacc ttgagctgcc ggatggcctg accaaacacc
    46441 cagtcaccaa tggcatgcat caggccgatt tcctcggcga ccgggatgaa ctgaaccgga
    46501 ctgatgaaac cgcgctcggg gtgattccag cgaatcagcg cttcgcactt gcagatctgt
    46561 cctgtgcgca ggtcgacgat cggttggtac accatcgaga actggttctg ctcgaccgcg
    46621 cggtgcagcg catcggcgag ccgcaggcga tgctgggcag acgcttcgag cgggtgttcg
    46681 tagtaatggg cgcgatcgcg gccggcgcgc ttggcctggt acatggccag gtccgcgtgt
    46741 cgaatcaggt cgtcggcgtc ctcgccatcc tggggaaaga ccgatacgcc gatgctgcac
    46801 gacaggaaga tgcgctcgtc gccgacttcc atcggctcct tcaggtgcag catcaagcgc
    46861 gcggtcacct ggtcgatgtc gtcatcggac tcggcacggc tcagcacgac gccgaactcg
    46921 tcgccgccga atcgcgccac cgaatccttc ttgcggatcg attccctgat gcgagccgcc
    46981 gccatcgcga tggcctggtt gcccgcgttg cgccccatca cgttgttgat ctgcttgaag
    47041 tggtcgagat cgatgaacag gaccgcaatg cgctggctct tgcctgcagc gtccttcagg
    47101 atctcctcga gcttctcggc gaagttgcgg cggttcggca tcccggtcag gttgtcgaaa
    47161 ttggcctgca gccacatcag cttctgattg cggaatcggt cggtgatgtc gcgagcgatc
    47221 tgcgaggcgc cgatgacgat gccgtccgtg ttgaatatcg gcgaaatgtt gaccgacagg
    47281 ttgatcagcc gaccgtcctt gcgtcgcctg acggtttcga gctgctcgac ccgttcccct
    47341 gcagcgatgc gcgtcaggat cgatggctcc tcgtccggcc gatcctcggg aatgatcgac
    47401 cgcatgggtt tgccgatcat ttcttcggag gagtagccca gaaggcgctc tgctgcggga
    47461 ttccagcttt cgatgattcc ggtgagcgac ttgctgatga tgacgtcgtc ggacgactcg
    47521 acgatggctg cgcaatgtcg gaggtaggac gcctgctgtt cacccccgac agaagcgggc
    47581 gctgccacct gcgggccggg acccgggtgt tgcaggcttt cttctccggc ggattcgtcc
    47641 tgtctgaaat acacggcctt gattcctcct ggatgcgggt tcagcgcgaa accccgcgcg
    47701 tttcgtccca ggcctcggaa cgatcaagca tcgacgaatt tcaactggtt cggtgcgatg
    47761 cgttgcagcg aactgcgcaa cgaactgtgc aacgaaccag ggcaagagac gaatgccctc
    47821 gcacgccctg ttgtatcggc tcgttcggca gtttctgaat caccgcccga gttgcgctct
    47881 ccgggtggac attgatctgc aggcccggag gggtaattat cgtttgtatc aattattctc
    47941 cgatctggag ttgtgatgga gccgcggagg cgacctcagt acatgaactt tgcggccggc
    48001 tttcccgatg ggcgagtcag gatcccctgc gtaccctcag gccagaggtc agttcccgga
    48061 gtgaagcgag ctgacagcca ggtcaccggc acgaccagcg tgattttccc cacgtgacca
    48121 gcctgttcgt cgtgatgcta aggagtgcgg tacgcgaatc acatttatca tttaacaaat
    48181 gatgaaagta aattccgtgg ccccccccgc ggtaggtcct ggagcgccga ccaggctcgc
    48241 tgccctgttg catccggtct caagactcct ccggaccctg acgtctcacg ctggaaaccc
    48301 gtttcgacat gcccaaaacc atcctgatcg tcgatgactc ggcctccctg cgcaccgtca
    48361 tcaagctcgc gcttgctcgc gacggctacg acgtcatcga agccgcaaat ggtctggagg
    48421 gggtggctcg actcgagatg accggcaagg tccatctcat cctcggcgat gtgaacatgc
    48481 ccgacatgga cggcatcgct ttcgtcgagc acgtcaaggc acatcctcga catggcgcga
    48541 cgccggtcat catgctgggc acagcgggtc aggatgctcg aaaggaacag gcccgggcgg
    48601 ccggtgccaa ggcctggttc acgaagccct tcaacacgtc ccagttgctg ggcgccgtgg
    48661 gcaaactggt cctgccctga ggccgcctga cttggtcaaa gcgaagggcg aggccggtca
    48721 gcaagactct gggcgggagg ccctcggagg gacgtggagc gggtgaaggg aatcgaaccc
    48781 tcgtatgaag cttgggaagc tgccgttcta ccattgaact acacccgctc agtgcggcgt
    48841 tgattctagc ggggcacgct gctgacggac catccgggtt gcacgtgctg cctctgcggc
    48901 cggtgcgcga ccgcctgcga agtcggcctt cggcgcccac ctcggtcagg tcgcacttca
    48961 gcttggcttc aggccgccga tatcgatcgc aagtccgtgg ccccgatgtg gagcctggac
    49021 gcccgacgac attcatcgct cacgcccgag atccccagat gcgcttgccc gttctgtcca
    49081 ttcgaagcac cgtgctgctg ggcatcgcca tggcggtgct cgctcccacc atcaccctct
    49141 ggcatcttga cgagcgattg actcgcgccg cgcaggagcc actgatcgcg cagaaccgtc
    49201 aggccgtgct ggtgatgacc tcggcctcgc tggtcgaacc catgtggacg ctggacgaag
    49261 agaagacccg gcagatcgcg cagaaggtgc tcgaagaggc ttcggtcctc agtctgaggt
    49321 tgaccgaagg ccgcccgctg tcggtgccga cggttcttgc acgccagggc agcgagttgg
    49381 gccagggtgt ggccctgaag acgacgatct cccgtgaggg ggagaacctg ggcgaactgg
    49441 aaatctggtt cgaccccgat cagatcgagc gcgcgcttgc cgaccgccgc agcgcgacga
    49501 tcctgctggc tgcgctgcag gtcctgctca gcgtggtcgt gctgcttgcc gtgctgtatc
    49561 gccggttgct cgtcccgatc ggccagctca agcggcaggc gagcgacatc gcgtcgcggg
    49621 cggatgtggg tccgatcgcg tgggatcgac gcgacgaact gggtgaactg ggtgagcacc
    49681 tcaacgaggt tcatgtgcac atcgaccagt tgttcgatca gctcgaggaa cagaaggccg
    49741 agctcgagaa ggtcgccctg cacgatccgt tgaccggcct ggccaaccgt gccttgttcg
    49801 gcgagctgac gcgtgccgcc gtggcggccg cgcagcgcga cggcggtcgg ctggcgctgc
    49861 tgttcatcga cctggaccgg ttcaaggcgg tgaacgacac cctcgggcat gcggccggcg
    49921 actcgttgct ggtcgcgctg gcagagcgtc tgcgcggtgc gatgcgcgcg tcggacgtgg
    49981 tgtgccgaca cagcggcgac gagttcaccg tgctgctgcg cgatgtgaac cattgggacg
    50041 aggtggcatc cactgccgac cgtctgctcc aggagatcga gaagccggtc tacatcgggt
    50101 cacggctggc cggtgtatcg gccagcatcg gggtcgccct gtttcccgac gatgcgagtg
    50161 atcacgagac gctggtgagc cacgccgaca ccgcgatgta cgtggccaag agcctggggc
    50221 gagggcgctg cagcttcttc cgcgcagagt tcaataccca gctgctggcg aacctgcagc
    50281 tcgagcagga aatgcggcag gcactcgaac gcgacgagtt cgtcctgttc taccaaccac
    50341 aggtcgatgc cggcaccggc gtgctgtcgg ggtgcgaggc cctgattcgc tggaggcatc
    50401 ctgtgcgcgg cctggtgccg ccgctggaat tcatcccggc ggccgaacag tgcggcctga
    50461 tcagcgagat cggcgcctgg acgatccgta cggcctgcgc ccagatcgcg cgctggaagc
    50521 gcagtggcat ctcgttcggc agtgttgccg tcaacgtctc ggcgctggag ttccgcagcc
    50581 accgcctgct ggatacgttg acttcggcga tggccgattt cggagtccag ccgcacgaac
    50641 ttgaaatcga actgacggag accgtgctga tgaccgacac cgacacctcg caggtgatcg
    50701 tggaccggct gcatgcgctg gggctgccgc tggcggtgga cgatttcgga accggctatt
    50761 cgtcgctggc ctatctgaag cggctgcatc cctcgaagat caagatcgac cgcagcttcg
    50821 tgcgcgacct gcctgacgac agcgatgatc gcgttctggt gcaggccatc gtgcagctcg
    50881 ccgcggcgat gggcatccag gtcattgccg aaggtgtcga gacgcaggct cagcgcagct
    50941 tcctgcacgg tatcgggtgc ggcctgcttc agggttacct gatcagcagg cctcaggaag
    51001 ccgacggttt cgagcggttc gtgcgccagc gggccttcga gcgcaacgcc gttctgccgg
    51061 agccgatcga cgcctgacgc tcgaaggccg gcagcccgca gaagtcagag cctttgccag
    51121 cggtcgaagg tggccgcctg gccgtggcac atcccggacc catcgatcag gttccacacc
    51181 accgcctgct cgatgcgaaa acgcctgccg gtggccgaga tgcggatgcc tgcgtagtcg
    51241 gtgatgcacc cgtcctccgt gacccgctgc agcagacgcg cacgctcctc tcgcagcagt
    51301 ggctcggcgg aaagtcgcga gggcatgccc acgaacgaag cccagtccat ctcgaagacc
    51361 tcgagtgcgc gacggttgcc gtagaagaag atcggatcgg gctcggtgcc gtgcgcgacg
    51421 atgacgcacg gtgcattcca gagcgccgcg cgcagatcgg cctccgacgc aacgaggaca
    51481 cgcccggtca gccgctgatg gctctcggtc agcagcgcaa gccgctggtc cgagggttcg
    51541 agcggaactg tcggcaggtg atcgccgagg gtcagagtca tggtgtgcga accgggtcgg
    51601 tgcggggatg cgacggaaaa ccgaggccgg attctcgctc ccgatctgcc ggcctccggg
    51661 aaggttccga caacgctgca atacgtctcg aatactcaca cctgatcgag ggactcgccg
    51721 ccacaatccg tgagtgaccg acggcgagac gacgaggaag accctgatga gcgacgcttg
    51781 cgtgcatgtt ctgcaactgg ggccgggcca tgccgccgag gcgctggccc gtgccgatcc
    51841 aggctacggg cctttccagg tcaccgcttg ctcggccggc gacgtgtggg agcgtctcgc
    51901 agcggcaaga gtcgatgtgc tgatcgtgca gggtgcctgg accgactgga cacggtgcgc
    51961 ttccgacgtg gccgtggtcc tggtcgtgga tcaggtgagc gccgccgatt gcctgacgtg
    52021 gttgcaggaa ggcgcccagg aggtcgtgac ggccgccgag ctggtcgatc cgtcctggtc
    52081 gcgcagggtg cgtgccgcgg tggagcggca gcgggtgcgc gccgccacgc ggcaggcctt
    52141 tgcaaccgac gtgcacaccg ggctgccgca tgtcgggcaa ttgatcgagc acatgagtca
    52201 cctgcttgcg ctgcgcgaac gcgaacctgc gccgatggcg ttgctcgtgt tgcgcatcga
    52261 ggggctggcc accacgatgg ctcggctggg catcgaggcc gctggcacgc tgcggcgcaa
    52321 ggtcgccgtg cgtctgcgtg ccggcgtgcg ggccagcgac gtggtcgcct cggtcggcga
    52381 cgactccttt gcggtgctgc tttcccagat cgacacccct gcagatgccg agcgggtcgg
    52441 acgcaagctc ctgggctcgc ttggagagac gttcaaggtc ggcggcagcg atctggcggt
    52501 ggcgaccgca ctgggcatcg cggtgtatcc atccgatggc acgcagccgg acatgttgct
    52561 gcgccgtgcc gccggcctgg cagcttcggc acaggcgacc ggccgatcag gctattccaa
    52621 tcgactggag cgaggcgccg cgggatctgc tgccaacgac gaagcgccga attgaggccg
    52681 ggtcaccaga agatcgccca caccagcgcg ccgatcatca gccacttgcc gacgtagtag
    52741 ccggtgcggt tgccgcgctt gaattccttg gccatggcgc gcagcttgta gccttgcgcg
    52801 aacaccttgt tgacgccgcc gatcggctcg ccggccacgt tctgggtcga ggcggcagcc
    52861 atcacgtgct tggcaaagaa gcggttgaac cagcgcagcg ggctgcccca aagcgggcgc
    52921 tcgacgtcgc agaacaggat cacgcgcggc tgacctgtgg tgttggccgc atggtggatg
    52981 taggtctcgt cgaacatcac cgcttcgccg tcgtgccagt ggtagcgctg accatcgacc
    53041 tcgatgtagc aaccgggatc gttcggcgtc tgcaggccca ggtggtagcg cagggagccg
    53101 gcgtatgggt cgcggtgcct caccagcgtg gcgccgggtg gaagcgacgc gaacatcgcg
    53161 gccttgatcg acggaatgcc gcgcagcagc ttcacggtct gcgggcacag cgcttctgcc
    53221 gaggccaggt ccttgccgta ccaggtcagg tagaagcgct tccagccggt gcggaagaac
    53281 gagttgaagc ccatgtcggt gtagccggcc gccgcgcgga tgtagccctc gtcgttcagc
    53341 cgcagcgcct cggaccggat ggtctgccag ttctgctgca ggggcgccat ttcagggaac
    53401 tcggccagtt cgatgtaggg gctgcgctgg gtgcgcgaga acaggtacat cagcacgttc
    53461 accggtgcca ggaacaccgt gaagtcggtc agcgcgcgca ccaggcggaa gcgcactcgg
    53521 ccgcgcaggt gcacataaag tgccgatgcg atgaaggtca gcaggaccca gtggcggatc
    53581 tcgagggacg ggatgttcat ggtctgttca acgctggatg tcgccgaggc acaggtactt
    53641 gatctcgaca tagtcgtcga tcccgtggtg cgatccctcg cgccccaggc ccgactgctt
    53701 cacgccaccg aacgggacct cggccgtcga gatcagcccg gtgttgacgc cgaccatgcc
    53761 gtactcgagc gcctcgccga cgcggaagac tcgtccgatg tcgcggctgt agaaatagct
    53821 ggccaggccg aactcggtgt cgttggcggc ggcgatcgcc tcggcctcgg tgtggaagcg
    53881 gaacaccgga gccaccggtc cgaaggtttc ctcgcgcgcg cacagcatgt cgccggtggc
    53941 atcggcgagc accgtcggct cgtagaagcg gctgtccctg cgctggccgc ccgccagcag
    54001 ccgcgcgccc ttggccaccg cgtcggcgac gtggctctcg accttggcga tcgcgttcgc
    54061 gtcgatcatc ggtccctgcg tgacgccggc gtcgaatccg ttgcccagct ggatgccgcg
    54121 caccttcgcg gcgagcttct ccacgaaggc gtcgtacacg ccgtcctgca cgtacagccg
    54181 gttggcgcag acgcaggtct ggccggcatt gcgatatttg ctgaccatcg cgccctcgac
    54241 ggcactgtcg atgtcggcgt cgtcgaacac gatgaacggc gcgttgcccc cgagttcgag
    54301 cgacagcttc ttgatcgtcg gcgcgcactg cgcggccagg atgcggccga cctcggtcga
    54361 cccggtgaag gacaggtggc gcaccgtgtc gctggcgcac agcacccggc cgatggcgat
    54421 cgatcgttca ctgtcagcgg tgacgatgtt gagcacgcct ggtggcaggc cggcgcgctg
    54481 cgccagttcg gccagcgcga gggccgacag cggcgtctgt tcggcaggct tgatgaccac
    54541 ggtgcagccg gccgccagcg ccggcgcgac cttgcgcgtg atcatcgcga tcgggaaatt
    54601 ccagggcgtg atggcagcgc acacgcccat cgcctgcttg atgacgaggt agcgcttgtt
    54661 gacgtcggtg gtcggtacgg tctcgccgta gacgcgcttg gcctcctcgg cgaaccattc
    54721 gatgaagctc gcgccataga ccacctcgcc gcgcgcctcg gccagcggct tgccctgttc
    54781 ggcggtcatc aggcgggcga ggtcgtcggc gtgctgatgc agcaggtgaa accacttcat
    54841 caggatcgct gcacgctcct tggcggtctt gacgcgccag gcgggccagg cacgctcggc
    54901 ggcagcgagc gcggcctcgg ccccggcagc tccgaggttc gcgacatcgg ccagcttgcg
    54961 tcccgtggcg gggtcgtcga ccacgaaccg gtcggcggcg ctgacccaga cgccgtcgat
    55021 gaaggcgtcg gtcttcagca gcgtggggtc ggtgagtgcc gccagcggcg aggtgtggtt
    55081 gtccatgctt gctcgaaatc gtgcggtgag ggcttccggg tccagacctg gccagccggc
    55141 cgccgcgggg cattcactct accgcttccc gctgccgtcc gagggcgggg gataaacttg
    55201 aaaccatccc accagcttcc ctttcgaggg tgactcggcc ccgtgcgcgc ctgccgtgca
    55261 gcgccgatct tcacctgcga cacgctccca tgaaagccac cgacatccgc cagaccttcc
    55321 tgaagttctt cgaatcgaag ggccacgcca tcgtgccgag cagcccggtg attccgggcg
    55381 acgacccgac gctgctgttc accaacgcgg ggatgaacca gttcaaggac gtgttcctcg
    55441 gcttcgaccg tcggccgtac acccgtgcga cgaccagcca gaagtgcatc cgcgccggcg
    55501 gcaagcacaa cgacctcgac aacgtgggct acaccgcacg gcaccacacc ttcttcgaga
    55561 tgctcggcaa tttctcgttc ggcgactact tcaagcacgg cgcgatccag tacgcctggg
    55621 aattgctgac cgaggtctac aagctgccca aggacaggct ctgggtgacg gtctatgccg
    55681 aagacgacga ggcttacgag atctggaaca agaccgtcgg cgttccggcc gagcgcatcg
    55741 tgcgcatcgg cgacaacaag ggcgcgcgct acgcgtccga caacttctgg atgatgggcg
    55801 acaccggacc ctgcggcccg tgcagcgaga tcttctacga ccacggcccc gacgtggccg
    55861 gcggcccgcc cgggtcgccc gagcaggacg gcgaccgcta catcgagatc tggaacaacg
    55921 tcttcatgca gttcaaccgc accgaagacg gcgtcatgca caagctgccc aagcccagcg
    55981 tggacaccgg catgggcctc gagcgcatca ccgcggtgct gcagcatgtg cacagcaact
    56041 acgagatcga cctgttcgtc aatctgctcg atgcggccaa gcgggcggtc gatgcggccg
    56101 gtgccgcaga ctgcgacaag gactcgccat cgctgaaggt catcgcggat cacatccgcg
    56161 cctgcacgtt caccgtggtc gacggcatca tccccggcaa cgaaggccgc ggctacgtgc
    56221 tgcgccggat cacccgccgc gccatccgcc atggctacaa gctcggcgcg cgcacgccgt
    56281 tcttccacac gctggtcgcc gcactggccg tcgagatggg cgacgcctat cctgaactgc
    56341 ggcagcagca acagcgtgtc accgaggtgc tgaagcagga agaggagcgc ttcttccaga
    56401 ccatctcgaa cggcatggaa atcctcgagg cggcgctggc cgagaccgct gcgaagccgg
    56461 gtgaagccgg gcgcatcctc aacggcgatc tcgcattcaa gcttcacgac acctacggct
    56521 ttccgctcga cctgaccgcc gatgtctgcc gcgagcgcga catgaccgtc gacggcgcgg
    56581 cgttcgacat cgcgatgaac cggcagcgcg agcaggcgcg cgccgctggc aagttcaaga
    56641 tggccctggg cctcgaatac agcggcaccg caaccacctt ccacggctac gaccacctgg
    56701 tgcacgaggg cgcgaaggtc accgcgatct acctcgacgg cacgccggcg ctgcgcgccc
    56761 gtgcaggcga cgacgcggtg atcgtgctcg accacacccc gttctacgcc gagagcggcg
    56821 gccaggccgg cgacaccggc gagctgcgca acgcgcgatc ccgcgtgctg gtcgaggaca
    56881 cgatcaaggt gcaggccagc gtgttcggac accacggacg catcgtcgaa ggcgaggtcg
    56941 aggtcggcga cagcttcacg gcgcgcgtcg atgccgagcg ccgcgtgaag accgtgcgca
    57001 accacagtgc cacgcacctg atgcacaagg ccttgcgcga ggtgctgggc gcgcacgtgc
    57061 agcagaaggg ttcgctggtc aacgcggagc gaacacgctt cgacttcgcg cacaacgcgc
    57121 cgatgaccga ggcccagatc gcgaaggtcg agtcgctggt gaacgctgag gtcctggcca
    57181 acgcggcgac gcaggcgcgc gtgatggcga tcgacgatgc gcagaagacc ggcgcgatga
    57241 tgctgttcgg cgagaagtac ggtgacgagg tgcgggtgct cgacatcggc agcagccgcg
    57301 agctgtgcgg cggtacgcac gtgacgcgca ccggcgacat cgggctgttc aagatcgtcg
    57361 ccgaaagcgg tgtcgccgcg ggcgttcggc gcgtcgaggc gatcaccggc gacaacgcgc
    57421 tggtttacct gcagtcgctc gaaggcacga tgaatcagct ggccggcacg ctgaaggcgt
    57481 cgccggccga agtgccgacc cgcctgacgc aggtgctgga tcaagtgcga gcgctcgaga
    57541 aggaactggt cgcgatcaag ggcaagctgg cctcgtcgca gggcgacgag ctgctgtccc
    57601 aggcggtcga tgtcggatcg ctgaaggtgc tggccgcgcg tctcgaaggg gccgacgcca
    57661 aggcgctgcg ggagacgatg gacaagctca aggacaagct caagagcgcg gtgatcgtgc
    57721 tggccagtgt cgaaggcggc aaggtgcaac tcgccgcagg cgtcaccgcc gaccacatcg
    57781 gcaaggtgaa agccggcgaa ctggtcaact tcgtcgcgca gcaggtcgga ggcaagggcg
    57841 gcggcaaggc cgacatggcg atggcggggg ggacggatcc gagcgggctt tctgcagctt
    57901 tgtactcggt ccgggcttgg gtggcggagc gggtttgact ctcgtctgat tgcctggcag
    57961 gcaccgcgtc gggttccgcc ccgacaggcg ggtaactttc ttctgctgcc ccagaagaaa
    58021 gtcaccaaag aagaggggcc gaacac
//
LOCUS       DAOORV010000009        53429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_695_length_53429_cov_9.566737, whole genome shotgun sequence.
ACCESSION   DAOORV010000009 DAOORV010000000
VERSION     DAOORV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 53429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_695_length_53429_cov_9.566737"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2554
                     /locus_tag="PKV56_02480"
     CDS             <1..2554
                     /locus_tag="PKV56_02480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOX66704.1"
                     /translation="LTEAAETMRRGGGVGYDFSRIRPSGAWVGSTRSSASGPVSYMRV
                     FDRSCETVESAGARRGAQMGVLRCDHPDIEAFIRAKDGGDLKNFNLSVGVTDAFMQAV
                     QADGEVELVHRAEPGVAQKEAGAYQQAQPGGGGLWVYRKRRARELWDLIMRSTYDHAE
                     PGVLFLDHINRDNNLSYCETIASTNPCAEQPLPPYGCCCLGSIDLTRFVREPFEDQAR
                     FDEASFISVAQVAVRMLDNVLDVTVWPLPQQQEEARHKRRVGLGFTGLGDALVMLNLR
                     YDTEPARAMARRISELMRDAAYEASVELARERGSFPLFNPDLYLSGTTFASRLPQGLR
                     DRIRSHGLRNSHLLSIAPTGTISLAFADNASNGIEPAFSWTYTRKKRMPDGNFKEYAV
                     EDHAWRLYRHLKGEKAPLGNAFVTALEMSAKAHEAMVAAVAPCIDTAISKTVNVPADY
                     PYEEFQHLYLEAWQSGLKGLATYRPNSVLGSVLSVTPAAAAPLQIDDKNRRLALERLP
                     APVLSSLRWPGRPELPGGNPAWTFMVNHPFGSFALFVGELAPESGGAPQPFEVWVNGA
                     EQPRGLGAMAKTLSMDLRANDPAWLQLKLDALATVAEERAFEMPFPPHGEKRLFPGVV
                     AATAAVVRWRCEQLKALPRLGHKAPTPVIDAMFSRDEPHTGPSGTLAWAVDIDNPATG
                     EAFTLTLKEVTLPGPDGGTVTRPCAVGFSGNYPRALDGLARLLSLDMRVIDPAWIGMK
                     LRKLLNYAEPLGHFMAFVPGLPHGERRQQTWPSTVAYLARLIIHRYAMLGVLDEEGYP
                     LRDMGVLESSKPRGERDPVMAGKVCLECGNATVIHKDGCDFCTACGAVGQCG"
     gene            2912..3175
                     /locus_tag="PKV56_02485"
     CDS             2912..3175
                     /locus_tag="PKV56_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66705.1"
                     /translation="MELHSVRIGSEHRTVFEMKTLADEMGRSISAGELLEAVFGQRIP
                     QGLCISARQFQGPVATFQAFIELQVSACESSPASGLKVSCGVN"
     gene            complement(3230..4006)
                     /locus_tag="PKV56_02490"
     CDS             complement(3230..4006)
                     /locus_tag="PKV56_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX66706.1"
                     /translation="MLLDGTEVRLGGRAFDLLWTLLEKRGNVVPKQYLHDAIWPGVCV
                     TPNNLDVQIWALRQRLGQDAISTVPRQGYMLTDAIEFAVVGSASRAPQPQPNRSDTIG
                     SDPGELMAQSLARRLKKLGQLALVGADASTRQMLCSAICKAYLRLGSGVIWRIEVQHA
                     TDLARLEQAIGRMRRVGGLMVVSEGDASLRTSVHEWTRMTDGDSGALNCLTTAAEAIA
                     GIDRHVHAVTPLKDSTGRARSDDASRSHLRWHSRGPGYPQ"
     gene            complement(4155..4922)
                     /locus_tag="PKV56_02495"
     CDS             complement(4155..4922)
                     /locus_tag="PKV56_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX66707.1"
                     /translation="MAIELYNQNGHVCLMFAHLSDEGGEAVQANQFLVIHGDTGAIID
                     PGGNVAYNELLLTIGRFFPPSKLSKILASHADPDIIASLDRWMTSTQATLYISSLWER
                     FAPHFCKPGKTQGRITGIPDAGMRIPLGRSELIALPAHFMHSEGNFQFWDPVSRILFS
                     GDLGVSLGTSADATRPITSLAAHVQRMEGFHRRYMVSGKVLRLWAQMARTLPIDMIVP
                     QHGAPLVGPAVQEFIEWIENLSCGVDLVTQASYTVPA"
     gene            5256..6692
                     /locus_tag="PKV56_02500"
     CDS             5256..6692
                     /locus_tag="PKV56_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX66708.1"
                     /translation="MTNTVTESFLQEEGWLQLRQMVDASALVQVKALVDQHADDFADD
                     FYDRMLADPAMGRFLDHEIVNRRLHASMVRWLRFVFDHESPVSQLQEMQRRTGEVHAR
                     IGVPHAAVAQGARVLKRALSARVLADARLPALSAEAVQYVYELFDLAMDVMGESASAD
                     EARLLRSDESYRLFFLSQDLRAERERQKSQLLEWANQILVNNYWDVQQENDGDADPGG
                     TVSQFKLWVEHKALMLFEGVPETALLRECIDATEAELLPRLKAARDHHASAKEVVSEL
                     NAAVERMKGLLGAMFDHAARLEDGRDELTRLLNRRYFPSIVKREIELAQVGGHPFALL
                     LLDLDRFADIGHALGSDAVDAVIAQIADKLSGSVRAGDFVFRVSECRFLVLLVEANDR
                     EAMTVAEELRVRIEHLPLRTPAGVAPQVTVSIGVAFFDGHPDYQQLLDRAERAVREAR
                     SFGGNRVERAEPSSPSACPFARSQFPKA"
     gene            6959..8296
                     /locus_tag="PKV56_02505"
     CDS             6959..8296
                     /locus_tag="PKV56_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOX66709.1"
                     /translation="MTRIKLCLAGLIGLLSALWLLADTLLPQPFTYFSFRSVFMQYSG
                     VLTVGVMSVAMLLALRPRWLERPLDGLDKMYRLHKWLGITALVLSVLHWWWAQGTKWM
                     VGWGWLVRPARGPRPDRSQLDTVEAWMSGQRDLAESVGEWAFYALLVLLVLALVKRFP
                     YHLFVKTHKLIALAYLVLVYHSVVLARFSYWSEPIGWALAALMASGGVAAVLVLLGRV
                     GAGRKVQGSIESLVHYPGLDVIESSVALGPGWPGHTAGQFAFVTSDRREGAHPYTIAS
                     AWNAQDGRITFITKALGDHTRRLREHLKVGMPVTVEGPYGCFDFEDDRPRQIWIGAGI
                     GITPFVARMKHLRDRPGRASIDLFHPTAVHEPAAIAKLHADARAAGVAVHLLVDARDG
                     RLDGERIRASVPDWADASIWFCGPPSFGRALRKDFVARGLPTGRFHQEMFEMR"
     gene            8314..8868
                     /locus_tag="PKV56_02510"
     CDS             8314..8868
                     /locus_tag="PKV56_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX66710.1"
                     /translation="MKSAFPGFDSPAVGFEQPFEMLLACHERVRRSLSLLGRLVSRVG
                     ERGHDDATRSAASDVLRYFDLAAPLHHEDEETHVFPVLAASVDAELVAAASSLQADHL
                     RMTALWSAIRPTLEAWSAAHASGTAEADFLDRAADFERIYEAHLAMEERLVFPAARER
                     ISASLLAAMSSDMARRRGAPVGPR"
     gene            complement(8902..10134)
                     /locus_tag="PKV56_02515"
     CDS             complement(8902..10134)
                     /locus_tag="PKV56_02515"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOX66711.1"
                     /translation="MISTEERVAEAHDLTQVLRRSGLKPLVLGGRSLLPIVQGGMGVG
                     VSAHRLAGTVAGLGAVGTISSIDLRRHHPDLMELTRGIAPGPAGTAAAKRAIDDANLV
                     ALEREIRAAQALSEGRGLLAINVMRAVNEYGPSATRALECGIDALVVGAGLPLDLPDL
                     AVDHPRALLIPILSDARGVQLLVRKWERKKRLPDAIVIEHPGLAGGHLGAARVSDLTS
                     PHFDFDVAIPQIREFLRSAGIEKEIPIITAGGIRCFDDIARLQSLGAAGVQLGTPFAV
                     TTECDAHIEFKRVLANANDEDLIEFTSAAGLPARAVSTPWLQSYLKAEQRLQEVAHPK
                     PRCTLAFDCLKQCGLRDALPGWGQFCIDTQLAAAMRGDVKKGLFFRGVGRLPFGNEIP
                     SVRELMQRLLTPGTTTAG"
     gene            complement(10231..10713)
                     /locus_tag="PKV56_02520"
     CDS             complement(10231..10713)
                     /locus_tag="PKV56_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOX66712.1"
                     /translation="MTHCAARLVAILLLAVCTLAGAGVAVPVAADPALESRVNTLGAE
                     LRCLVCQNQSLADSHAELAVDLKNQIREQLRAGRSEQQVIDYMTERYGDFVLYRPPLK
                     ASTVLLWAGPALLVLLGLIALWRSLGRRPGTPEPPALSAADEQRALELLGDPRRDAGA
                     "
     gene            complement(10717..11244)
                     /locus_tag="PKV56_02525"
     CDS             complement(10717..11244)
                     /locus_tag="PKV56_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOX66713.1"
                     /translation="MSPRLLVPLCAFILLGALLGAGLRLKPREIPSPLIGKAAPAFSL
                     PLLSESQRALASADLKGKVWLLNVWASWCTPCREEHPFLVALAREQIVPIYGLNYKDD
                     PRAAQEWIQRLGNPYAESVLDRDGKVGIDYGVYGVPETFVIDRSGVIRFKHVGPLTRE
                     VWTRDLLPIVQQLRS"
     gene            complement(11241..13247)
                     /locus_tag="PKV56_02530"
     CDS             complement(11241..13247)
                     /locus_tag="PKV56_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOX66714.1"
                     /translation="MLPELGHFALIVALFLGVVLATLPLIGAARNDIAWMGLARPAAQ
                     GQFAFIAIGFASLMVSFANNDFSVVNVASNSNSELPMAYRIAATWGSHEGSMLLWVFM
                     LSGWTLAVSLLSRRLPLQMVSRVLGVMGFVSVGFLLFILLTSNPFERMNPPALDGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFAVAFAFAVAALLSGRFDAAWARWSRPWTTAAWMFL
                     TVGIALGSWWAYYELGWGGWWFWDPVENASFMPWLAGTALLHSLAVAEKRGAFKAWTI
                     LLAIAAFSLSLLGTFLVRSGVLTSVHAFATDPARGVFILSFLGIVIGGSLSLFAWRAA
                     SVGFSGRFDRVSRESLLLVNNVLLVAATGSVMLGTLYPLALDALGLGKISVGTPYFEA
                     VFVPLMAPVVLLMGVGPLARWKSASWADMASRLRWPAMASVAAALVLPWWAGRFSAGV
                     ALGLFLAAWVFTSTAALLLARLRGNTLGERWKQLGRSSRSFFGMVVAHLGVGIFIVGV
                     TMVRGFDVESDVKMSTGDHLDIGGYSIRFKGVSESTGPNYVAVQADIDVQRDGGDTFS
                     MKPEKRLYKVQQMPMTEAAIDTGFLRDVYISLGEPLDDKTWTLRVYIKPFVDWIWGGA
                     LVMALGGLLAATDRRYRVSRVQSESAESGLAGAAQRIDAQGATP"
     gene            complement(13257..13484)
                     /gene="ccmD"
                     /locus_tag="PKV56_02535"
     CDS             complement(13257..13484)
                     /gene="ccmD"
                     /locus_tag="PKV56_02535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HOX66715.1"
                     /translation="MNWHSVAEFAEMGGYGLYVWGSYLMAAATLAWEVVMLMQRRRRA
                     EDEIREDALINRAQSREGERRSRSDASSTWN"
     gene            complement(13481..14239)
                     /gene="ccsA"
                     /locus_tag="PKV56_02540"
     CDS             complement(13481..14239)
                     /gene="ccsA"
                     /locus_tag="PKV56_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOX66716.1"
                     /translation="MSVQMALHRTPSGEGESTRSRWERHASPQQLYPLAGRLMGWAWA
                     ISALLAAAGLSVGLLIAPAEGQQGDADRIILVHLPAAGLSLLIYLSMAFWCALALVLN
                     SRMASMMASALAPTGLLMTFIALWTGALWDKPTWGTWWVWDARLISELLLILLYIGFM
                     ALQSAIDDRRRMQRASTLLVLGGGLAVPATHFSDPWWSAPLHGASWSVTGMLLMTAAV
                     WAWSFAAALHRVRSIILESERHADWVRQQLELRR"
     gene            complement(14358..15401)
                     /locus_tag="PKV56_02545"
     CDS             complement(14358..15401)
                     /locus_tag="PKV56_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX66717.1"
                     /translation="MRFGAVLSAAISVLVLLGGCDATGSLPSARAAPSPETSTAAPSD
                     QHELGRKVYNFRCYFCHGYSGDARTLASNYLTPAPRDFTKADIASLPRSTIARAVRLG
                     RDGTAMKSFNGILTESEIDAVSAFVEREFVRDKAANTAYHTEANGWPGHERFSAAFPY
                     ARGQISISAPVESLDATQRRGRELFMTRCISCHDGPREDDSAPAWSARPLSFPRMGFV
                     PGQPNTPPVDAVSTSSVYAKHEVAPRIEGLGATERRGEALFQANCAFCHGADGSGKNW
                     IGQFMEPKARDLTAYSTQSMPRARLRQVIRDGLPGTSMPAWRSVLTSREIDDIAAYAA
                     RALFRRDEARAAR"
     gene            complement(15407..16489)
                     /locus_tag="PKV56_02550"
     CDS             complement(15407..16489)
                     /locus_tag="PKV56_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX66718.1"
                     /translation="MKPTDLERWRRLLAGLVLGLWMTASAWSAPGAEEPSNRAATLFH
                     NYCSVCHGDKGDGKSRASGSLSTPPRDFTSAASKQELTRERIVASITHGKPGTAMVAW
                     NRQLSADDIARLADHVFTRFVQPAPQAASKGRQIYERNCSVCHGDQGAGSMWARDNMA
                     RPPRDFTAPASQNLTREAMLAAVTHGKPGTPMVGFAGRLGESDIAEVVDYIRAALMSG
                     TAISGTRAHGGRDADQAVASQVAPSLVNMNVEFPEGLKGNRKRGGAFYLANCATCHGA
                     RGDGAGPRAYFINPKPRNFTEDASRARFNRVALYAAVSEGRLGSEMPAWKQVATPQQI
                     ADVSEYVFQSFVQGKATEVAGSPAQR"
     gene            16733..17851
                     /locus_tag="PKV56_02555"
     CDS             16733..17851
                     /locus_tag="PKV56_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOX66719.1"
                     /translation="MSALMCCRIPIGAVPMVLAVLLAGCAQAPPAPQVNPERTLVFPA
                     PPDAPRFAYERTIRSSGDIVPDEAAGELRRLVTGEDARGEPLSKPYAVAVHRGRIFVS
                     DTVSRFVRLFDVPAGKQYRIGDDDGAGQLIKPIGLDIDAAGNLYVADIGAKAIMVYGP
                     DRQFLRRIGGEKWFSRLSSVTVDPEGKRVYGVDLGGVSSEQHQVRVFDGQTGQHLMDI
                     GKRGGGAGEFNLPRDLAIGADGRLYVVDGGNFRVVVFDHDGRYLQSFGSVGKQYGQFA
                     RPKEIAADREGNVYVVDAAFGNFQVFNSDGELLMFVGDRSERDGPAKYMLPSGIAVDE
                     DGRIYVVDQWFRKIDVYRPVGLTTSSGFLARRAAPPPR"
     gene            18047..18937
                     /locus_tag="PKV56_02560"
     CDS             18047..18937
                     /locus_tag="PKV56_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66720.1"
                     /translation="MNLSRHPGIRRWGSATAGMLAALLLALSGSASAGISDTKHNLGS
                     GAGPAGRNQVSDTAEICVFCHTPHGGSTDAPVPLWNKRLGAVGAAAGGGSYTTYDTLQ
                     TPSLDGTVAAVGSISMACLSCHDGTQAMDNVINAPGSGGVLADGGGVDGRNYTWSGST
                     VSAAGRMNGGAALIGTDLSNDHPIGIQYCGGGLAGAGTTVSGTCKDGDFVAPQTQTIN
                     TNQVFWVDTGGPGKQRTDMPLYQRAAGGLGPMVECASCHDPHVSSAQAGPAGTGQSSG
                     ETFLRISNASSAVCTSCHVK"
     gene            19029..20057
                     /locus_tag="PKV56_02565"
     CDS             19029..20057
                     /locus_tag="PKV56_02565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX66721.1"
                     /translation="MAARMRVATGAVLWLLCAVVGAAETVVAPAAAASTPATAPFAVV
                     GADVISVADFQRALGVAMRKKYYHAKPPEAEFAKFQREVGDDVVNRVLLLNEARRRGI
                     QPDRARIDAAVKGYDAQYKDSANWKVNRDKMLAAVVPQLERESLLERLEVLVKAMPEP
                     APAVVRRYYDEHKELFIEPEQVKLSVILLKVDPSSPQALWNSARDEGQAVHKRLLAGA
                     DFGELARLHSGDRSAPQGGEMDYTHRGMLPEAIDKVVEKLRPHEVSEPVQVLEGVAIL
                     RLDDRRVARQRPFDQVRDRAADLWQRAEGESRWKALIAELRGAALIRIDESHYAPLCR
                     ASDKPCAS"
     gene            20173..22053
                     /locus_tag="PKV56_02570"
     CDS             20173..22053
                     /locus_tag="PKV56_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66722.1"
                     /translation="MLLPGTAAHGVAYLPGSAEPDPPTGDAPAAVPQAAEPLRAGSGI
                     EWKLAPWRMSGSVALDGRWLRLDNGNRSKQGVLVTDLEFTSYVWKPWFIQLRAGLGAM
                     ASVEHSSADSGSRSGSGSGSSALNGRFTLSAFPASRFPFELRADASDSQTDGNSLGGA
                     YRTHHVAVTQSYRPETGNDNYQLNIDYSKVMPQEGGADTLWAFQATALRQYVENTFEL
                     GANLSLNRRSDTGDSSDIRTLTARHSFHPTTDFLIETLATHNDVQLRSSVPGGDLDFG
                     SDMRQVSSFLTWRPREGEWFHAPDAPLSVTGTARWIDSGANNGGGGQRFSAFSGTVGV
                     TKELTRAWRVTGAVSTTRLDGGSNRAVTSATGNASVTYAPQGTELGRWRYAPTVSSNL
                     SVTRSSDSQDRRVIGLQASHSVSRDFLVAGSESLSLTLSESAAALRESPSADSAYAIS
                     HSAGVFWQVQGDGSSQSFASVSVSDSLTWAHGPGHFSLGNLQLSRRTQLTRYTSWSAN
                     LTLQATRNQSTEIDPFTGEHRKRGGGWQQFHSGSINYESQRVFSVPRLRFTAVLSANS
                     QQIERRSAGDIDAPRERITESLEGRLDYAIGRLDTRLSARVARIDERTVAAVYARVQR
                     RF"
     gene            22088..22504
                     /locus_tag="PKV56_02575"
     CDS             22088..22504
                     /locus_tag="PKV56_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOX66723.1"
                     /translation="MKFLRLLLATVALALALGSTVHAEYGDVVINNYSDAAGMRPVVF
                     PHWFHRVRFRCKVCHADLGFQFKAGGNEINMLKIIDGQFCGACHNGEIAWSVENCNLC
                     HSGKPNTGTHVHESTLQKLAAPAHAASAAAQAPARK"
     gene            22516..23529
                     /locus_tag="PKV56_02580"
     CDS             22516..23529
                     /locus_tag="PKV56_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX66724.1"
                     /translation="MRKLLISFLMTAAIPAFMADALAQDAKVSPKSIYDSTCSACHGP
                     GILGAPKFGDAAQWAPRAKAGIDALVKSAVAGTTKGMPPKGGRMDLSDAQLRGVIEYM
                     MAGGAPAASTAKAPAASTAPAAKAAVVASPPAPATKPAAPAAVAQPVATAAPGAVAAA
                     PNPPLTPEVNAFNRLLKPLGKFNRPAVESGIHDPANDMTLQLQPPAAAFAQLPKSMAG
                     NHVDWVKALDGKAIAPRWDRNDPAAAAIVMDLNIVREVKGSMPDVVYPHKQHTEWLDC
                     SNCHPAIFIPQKGANQISMASILLGQKCGVCHGKVAFPVSECRLCHSRKKAVAASAGE
                     AKP"
     gene            23526..24422
                     /locus_tag="PKV56_02585"
     CDS             23526..24422
                     /locus_tag="PKV56_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66725.1"
                     /translation="MRNDEPQASGGLRALAGVGLLVAAGMASAAEDAPPSQIDQRIRL
                     TAQLIGDSPAAQRILASGNQQAVSNLEAGRAHLAQAQDLLAKGDQSGALRHVDEALRH
                     VAVARRLVPDAPSRQAAARQRFEVQSRHLESMLGAWRVRLAPGDADDRELVEADGLVR
                     TALGLAHDGRHEEANQVLQKAERQILSGMSRLLNQRTLDYTARPTTLAEAFQVELARH
                     RELADLVPIALHDLKPDAEATTLIARYQETSQTLRNQALQRFEGGQTEDALAHLRNAL
                     LYLQRALNAAGVTTPEPTGSIQ"
     gene            24419..25282
                     /locus_tag="PKV56_02590"
     CDS             24419..25282
                     /locus_tag="PKV56_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66726.1"
                     /translation="MKNILAGLSLVFGLTLISVDAHAQDSVQLERRVQSIGTLIESSS
                     AARQIESSGVAEAREKRNNARLIHREAASTLGSGDLATAARLLDQAAREMMEGARLAR
                     PDQVTGEKTKRDFDARMESARALLAAQQRITKEKSAGREDQEATRSIESQIAGAQALA
                     SQNRYDEARPVLDRAYLTARVSIASMRRGDTLVRSLTFASPREEYDYEIDRNDTHRML
                     LTVLLSDRKDAAGAMPPNMQPFVDKAAVLRTEAEAQARRGDHGGAIKVLEDSTRELVR
                     AIRTGGIYIPG"
     gene            25292..25837
                     /locus_tag="PKV56_02595"
     CDS             25292..25837
                     /locus_tag="PKV56_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOX66727.1"
                     /translation="MKPLRRRPGRWLAAALVLLTAAALAATSLWTRLEKDGVHDPLGP
                     AIGVLQQPGEALESLPRDSAGNQVNWVKAIDSGAINPRTNILPETKVRVPDKDMIIAR
                     YGSMPAVKFPHRQHTLWLDCANCHDHLFKAEAGANRFSMMAILNGEQCGLCHGAVAFP
                     LTECNRCHSVSNASLPPRTAR"
     gene            25834..26205
                     /locus_tag="PKV56_02600"
     CDS             25834..26205
                     /locus_tag="PKV56_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOX66728.1"
                     /translation="MKRLRWIGLAACLWLQPQAHAEYGDVVLNKRSDQQGMRPVVFPH
                     WFHRIRFRCKVCHQELGFKMRAGANDVQMSDIIDGKFCGMCHNDRIAWGPDRCDLCHS
                     ALPRTPAGIVGGAQTSGPGRW"
     gene            26209..27141
                     /locus_tag="PKV56_02605"
     CDS             26209..27141
                     /locus_tag="PKV56_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66729.1"
                     /translation="MHRRHFLSIGLLGGCAAAAEDPVAPREARLLSPWRTVDGGFLAQ
                     PSLLAGLPPRPGAGMYVRLMASGALALRGNDLLVADMGTARLWRADVMLNTLTAIAGA
                     PVFPNTAVALGPDLSAWVLDTASRQVLRFGRDGRLLQTLRAGSATAMALADSGNTVLV
                     AESALSQWSELRAVGSRAVPVRPEIEGSERVIGVDGLALARNGLFVLDRAEAVVHRVG
                     REGRVLATLGAGELAQPSAIAADRSDRVFVVDEMQRVLRLLQDGQPAVTIDAEELGVQ
                     RIGGVAVDERLLAVSDSLTGRVVIHQLAAAATSR"
     gene            27138..28208
                     /locus_tag="PKV56_02610"
     CDS             27138..28208
                     /locus_tag="PKV56_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOX66730.1"
                     /translation="MRALLALILLVLAGCASDPGAASIRGELRYGMDDAPEGRKLMWP
                     APPEIPRFLYTGTLTGEHNFRRPAAETGGLKAFGRWILGLDDRPPAPVVLQRPGAVVS
                     DDHGRVYVSDTSRQAVFVFDEAAGELLVWDRAQGLVGFVAPSGLAVGPGRLYVADADL
                     AAVFVLDARGEPLSVIGRGLLKRPTGLARDPEHGLLFVADTYAHDIKVFTDDGVLVRV
                     IGGRGDRDGEFNFPTHLAFAQHELYVTDTLNSRVQVFNAEGELLLRKFGTRGLYLGNL
                     VRPKGVGVDSEGNVYVIESYYDSMLVYSNRSEFLLSIGGTGTATGRFYLPAGVWVDSK
                     NRVFIADMFNGRVVMFQFLGGS"
     gene            28208..30808
                     /locus_tag="PKV56_02615"
     CDS             28208..30808
                     /locus_tag="PKV56_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOX66731.1"
                     /translation="MRGLIRVLSLMAVLSGLMPASVADAQRVADVRGTRHNLSVDGLG
                     STHAAPGGTSEVCVFCHTPHAATQADLGGTPLRAPLWNRRVPAGSTYTPYTSSTMDAQ
                     SITDGLNAQPGGSSKLCLSCHDGTLAIGNVNVLNGNQNVTIPMTGTGPGGSMPVGEGT
                     ASGFTRFLGTDLRNDHPISVTFNSALATRDGELRLVDAQQRWPAGSGSVLGLRSPGFK
                     PLLPLEPTGSTGAGQVQCATCHDPHLRELDETKGNQKFLRAQRFQELTPSATHNASSD
                     IICLSCHDKNSGFGIWSYSAHARPDVAEETYRDGAATLREFPTGLPVWKASCLNCHDT
                     HTVQGARRLTREGTDAPLPAGNPLAARQGGNPALENTCYQCHTTVAKSALASTAGVPN
                     IESEFARAIRMPITTSDQGGAAEEVHDISSNFSDITIDCTTPNNRCGADLLEPKSQLA
                     QRHAECTDCHNPHRVIRNRLFNANPVSPDSKGTHAIDAVTGVTPTNLASGVLRGSWGV
                     EPVYGSPSFHVNPGNFTVKRGDPGANPSTAVTQPYMTREYQICLKCHSNHAYDNIPPS
                     LGRSGGTSPGTNGLTTYTNQAREFQPPLAHQGGGIGDPKSLGSNGGAGSAYTIGNHRS
                     WHPVMGQTGRDIDRRGISAAKNPWALPFRNAVGTQTMYCSDCHGSKATASGSLQPDSG
                     VSGPHGSENDFLLKAPFNASDPSLCFKCHEREVYSPTGDSRKSGFYTGFCCRGGRDDG
                     KDQLHWYHWNKILSHDPSRTLKCNWCHAAVPHGWKNKALLVNLNDVGEEAGFPGGNRE
                     FRMDSPSQAYNQEPYYLNAKLKVRTFATSGNWTDTNCGSNNSATTFGTNGNSTQSGRD
                     WMKDVCSNPP"
     gene            30805..31800
                     /locus_tag="PKV56_02620"
     CDS             30805..31800
                     /locus_tag="PKV56_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66732.1"
                     /translation="MRHGRIVAHFGSPRTTLAGLALLAVVVLAGSSSGGMVWPLTAVL
                     ALLSLNLCAALVVHPAFRRQLPLLTAHLALLALVVLAAVGRLCALDGRFELTQGVPFD
                     GRLIDQSRGPLHQERLQRLSFRHDGFEIDYAAGRKRGATRNSVTWVDADGAARSDVIG
                     DHRPLVLEGHRIYTSPNKGFAPVMRWEPRQGEPVVGAVHLPSYPMHELRQSREWTLPD
                     GRAVWVMLQFDEVLIDPQAAAAFRLPGEHRLVVRVGEVRAELAAGDRLDLDGGTLVYD
                     GLRTWMGYRVSYDPTLAWLLAASLLAALSLALHYALKFFGQAATAPRSLTGVIDG"
     gene            31793..32611
                     /gene="ccsA"
                     /locus_tag="PKV56_02625"
     CDS             31793..32611
                     /gene="ccsA"
                     /locus_tag="PKV56_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOX66733.1"
                     /translation="MAEGLALHSALALYAAAFGLAWMSLRTESGLQSGYTAAASLFGG
                     ALLLHTLSLALRWQAHGHGPFTTMHEILSSNLWSLALVFALAGWGVREVRATLWAVSP
                     VLLVMAIWMLLADARPGHMPPTYATPLLYLHTLVGKLFLGLLLVAVALGAVPWLRRTP
                     WGARRLARWPDDRRFDELAHRFSAFAFVCETLMLIVGAVWAQDAWGRYWAWDPLESWA
                     FVTWVALAATLHARIALRPTPAAHGLWLALVFAIAFLTFFGVPFISTAPHKGAV"
     gene            32608..33165
                     /locus_tag="PKV56_02630"
     CDS             32608..33165
                     /locus_tag="PKV56_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX66734.1"
                     /translation="MTRARTDRRRAGALLTLFAVFAGGFAFQWPGLEATRQSTESAAS
                     SRPLASATAQPEQRSAEVHRRFDDAVAMLQARDYNGAVTGFQRVLELAPDLPEAHVDM
                     GYALLGLGRAKEALDSFDGATALRPDQANAYYGMALAYEASGDLATATGAMRSYLHLA
                     RNESEAHLRRARAALWEWEARRTPR"
     gene            33311..34540
                     /gene="ccmI"
                     /locus_tag="PKV56_02635"
     CDS             33311..34540
                     /gene="ccmI"
                     /locus_tag="PKV56_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HOX66735.1"
                     /translation="MTVFWIAAALFLLAALLFLLPALLRPKADGFSAGGINLAIHRDQ
                     LREAEQDLSTGLLTLDRFQQSRAEIQQRVLEDTVALADRVMLASPARRTAIALAVLLP
                     LASVLTYLQLGDPEAAAPSLAPMQAPAGASARHSVNAEQIQKMVTALAEKLRADPNNA
                     EGWLMLGRSYTVLGRYQDAATAFRHASEQLPADAGVLADWADVLGMAQGKRLVGEPAR
                     LVQRALDIDPKNVKALALAGSVAFESRDYAAARGYWERLIAVVPPDSEMARSVGGSIQ
                     EAMQLEGSGGAVAAPAVASARQTVSGSVEISPELAARVEAGDTLFVFARAAQGPRMPL
                     AIVRRAATLPYAFTLDDSMAMAPNMKLSGQSQVVIGARISRSGNATPQAGDLTGQSAV
                     VSPGAQGLRIVIDQVQP"
     gene            34637..35233
                     /locus_tag="PKV56_02640"
     CDS             34637..35233
                     /locus_tag="PKV56_02640"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOX66736.1"
                     /translation="MNRSVAMRLPAAVFAVLCCAGTACAAAEDPPKAALDEARSASTE
                     LLTGVRSELLKAIDSSGPLRAIVVCKYTVPEISSAVSRKYGARVTRVSLRPLNPSLGW
                     GDAWEQKVLMDFDERVAKGEKPEGMEHFEVVNEPAGRFLRYVRALPLLPVCASCHGPA
                     DQISEPIRSQFAHDYPHGYSTGTPVGRIRGAVTFKKPL"
     gene            complement(35234..35761)
                     /locus_tag="PKV56_02645"
     CDS             complement(35234..35761)
                     /locus_tag="PKV56_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX66737.1"
                     /translation="MLLKRSIVGISLSAIIVCSYADPLNDAVAAVNRKDYQTAVRLLE
                     PLAKAGDPIAQTRLGLLYYHGHGVRESDALALQWFERAARKGLAEAQFHLGNMYAYGL
                     APLAAGTDPNRLAAQWYFEAARQGNADAQYSLGILFLTGSGVTQDPAEATKWIERAAA
                     LGHADAAVYLKGETK"
     gene            35843..37291
                     /locus_tag="PKV56_02650"
     CDS             35843..37291
                     /locus_tag="PKV56_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOX66738.1"
                     /translation="MNTIVADPGSELPKRKSLRAKGLVATLVLVAYLLGAVVYVSMER
                     AKIYESMQALDQLSQHEKAVALTESAVNGARIDVNMASNAAHSDPGMPEEISLYMESC
                     DKLFAALDAFDPSYTLLHRAITRSYEALEAAPDRSNWIDLRESLSRATDELAIRRANL
                     LEQREVLTLGYQRQYDAVTIESLALAALGVAVFGSIAAWFFSRLARDIGRLEDHAGQI
                     VRGTRGVALDVGREDELGRLMHAVNRMAVDLDEREKQIEVDAHRRFHQDKMLAVGALA
                     AGISHEVNNPLAVITGAAQEIGAMDGAELPQSVTDNARLILAQAERAAQAARRLAEVA
                     APQASERDWVDVNAMVRRVAQLIGYDRRYRHFVFDTDLDLQLPAIHVSGNAVQQVLMQ
                     MLSIACDAMVAGHAESKTLRLQTLLVDGQIEMQLLFPPVLDFDRPEVQRVLLLSRAII
                     EPLGGKLAFGQTAGPLQRIKLTLVAETGIEQG"
     gene            37297..38679
                     /locus_tag="PKV56_02655"
     CDS             37297..38679
                     /locus_tag="PKV56_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX66739.1"
                     /translation="MPELNVLVVDDEPAIRQVLSAAIGKAGYSVDAAGSAAEALAKLE
                     KASFDVVLSDVFMPVTDGIELLKLARERGHAATFIMVTAFASVDSAIEAIKAGAWDYI
                     TKPVRNEEILHRLEQIDALRGLREENRALRSLVMGSGEMAFAFTSPAMQANERLVSRV
                     APTDSTVLITGESGTGKGVTARRVHELSSRRDGPFVCVNCAAIPENLIESELFGHTKG
                     AFTGADRVRKGLFLQADRGTIFLDEIGELPLSLQTKLLHVLEAKEVRALGSEVSRKVD
                     VRIVTATNRDLAAMVAEGRFREDLYFRLSVFQIQMPPLRERRSDIPGLLRHLMAQRGV
                     PSGSSEALTLDPEAEDLLVNYSWPGNVRQLQNVLHRAAILADGGCIRVADLPAEVSHV
                     LPAVNEPAPVEGGECGLRERVRRFEHSLIRRAIDEAGGDRRLAAQRLGIGLSSLYRKL
                     EETAPESATG"
     gene            38741..39379
                     /locus_tag="PKV56_02660"
     CDS             38741..39379
                     /locus_tag="PKV56_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX66740.1"
                     /translation="MVGSKLRAWFAVALSLVSCLCQAIPSDDHARGVLAYQRGDVVAA
                     MSALREPARAGYAPSQALLAFILDRSDFSEEAFRLYRDAAAQNDAEGHAGLASLYFAG
                     RGVAKDEKLALEHFSKAADLGHAASIETVANAFLKRQMGLDADGPDGAAAVAALTRAA
                     GQDHVASAEALARAYQSGRYGLAADPAQAANWQARAVAIRKKQAAPVAKVSK"
     gene            39376..39675
                     /locus_tag="PKV56_02665"
     CDS             39376..39675
                     /locus_tag="PKV56_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX66741.1"
                     /translation="MNARSIWLPVALSWSVVAGAADALKGADLYRSHCSQCHGNDGRP
                     VLPTAPDFSRPTALLKSDLTLLASIRAGRGGMPAYQGQLRDREILDIVSHLRTLR"
     gene            39672..40715
                     /locus_tag="PKV56_02670"
     CDS             39672..40715
                     /locus_tag="PKV56_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOX66742.1"
                     /translation="MRSLLWRGLAVLVLCMPALVLAQKVRDAFDVGQSVYVRALTVEP
                     ATGALWVGTSAGVHEVDLGSGKLRNTFTRKHGLANEYVFAVGLDLQGNKWFGTNAGGV
                     SRYKNGKWKTFFPMHGLADYWVYSFAQQRGGDFWIGTWAGVNRVDAKTGKFSTYVKEL
                     VNEWVYGIAVDAQDRVWFGTEGGVSMYDGKRWRSWTHKDGLGAPNSDDLPFSTNTGLG
                     TRSRHDLSVSAEGPATYNPNYVFSILSAADGTVWAGTWGAGVSRWDGRAWTNLTTKDG
                     LAGNIVYSVAQGADGVYWFGTNNGVSRYDGKAFKNIGVAEGLLERNVYALAVAPDGDV
                     WVGTRRGVARISR"
     gene            40749..42014
                     /locus_tag="PKV56_02675"
     CDS             40749..42014
                     /locus_tag="PKV56_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOX66743.1"
                     /translation="MAINARIVGIALIALGAALAGAYQLGRGNAAANAAALPDSASTV
                     AAPAPTAASPVPGDAVQPPGHPHDPAGLQREGKVTGADPAQKFTHFRVGNKNVKRIYV
                     DGDTVWVGTSGGAVRYDTLTDDYKLFDIKSGLLSNGVFHVGKMGERVVVGTYGGGMSL
                     LDPKTQTWETFNIPEGLGDAFVYDVLTTKNGDVWVATWSGVNRIRGGALKDRSKWELH
                     TVQSTQGGLPNDWVYGLAEGRNGDVWLATEGGLAHYTEGRWENWNHEKGLGASYEMVK
                     NDIQYKNDPSKVSQHHAKQKQEMGLSNIDVAYNPNYIVSLAVDVQGVVWAGTWGGGLS
                     RFDGKTWKQFTVADGLPGNHVFMLHIDPKGQMWVGTNNGLAKMKDGKFEVLTTKDGLF
                     GNAVFSMATTRDGTLWIGSFGGVARIKPS"
     gene            42033..44780
                     /locus_tag="PKV56_02680"
     CDS             42033..44780
                     /locus_tag="PKV56_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66744.1"
                     /translation="MDRSTNHSLLHTLKPHNSGALLTLLLKIADDAVIVADADQQVVL
                     FNEGAERIFGYPAQAVLGQAISMLLPEAARAAHVDHLRKFALSPRAARRMGERRDISA
                     RRSDGSLFDAEASISHVELDGETYFTAILRDVSEARDAERALMRSEARFRGLAAAAPT
                     GIFQTDIDGNCCYVNDRWCEIAGMTAAESLGRGWLRALHPADRARVQLEWTAAVNEKR
                     AFHLSYRFLRPDGSEVWVVGDAVGSHESSGMPGGHIGTVTDVTESHHQALALERAKSE
                     AEAAARAKSLFLANMSHEIRTPLNAVVGMTTLLLDTAMTDDQRDFARTIRASGDSLLE
                     IINDILDYSKADVGKLEIEQRPFDLRRCVEESLDLVTPRALEKDLNLAYLIEEGTPEA
                     LVGDATRVRQIVVNLLSNAVKFTHQGEVFVFVDSERVDEETHRIHFAVRDTGIGISAE
                     HLPRLFQSFTQIDASTTRKYGGTGLGLAISRRLAELMGGNVYAESEPGQGSVFHATVL
                     VREAAFAEPAEFLLRNPPALVGKRILIVDDNQTNRRILTKMVLLWGMVPSTLPSALEA
                     MDRVRHGEAFDVAVIDMSMPDVDGLDLACEIRKRRSAQELPIVMLTSLGQRQLGQNAE
                     LAACHCKPIKASLLFQTLVEVIAGTAAPQDPIRPQARAEPTVPLRILVAEDHPINQRV
                     TQRLLRHLGHESDLAADGRKAIEAVAQRVYDLVLMDIQMPELDGLEAARAIVGARGTA
                     GLPRIVAMTANAMPGDRESYLAAGMDGYLAKPIELTDLAAVVSQAAVRAQARHHDGTF
                     DSVLDHARLEHLRSMQDENQPSLVRDLIDMFLTDSSHQVQQLNRAHAAADAHLMRGLS
                     HRLLSATQNLGASRLSRLCIELERLAKLGQTDAAGALLADLERERSLAHEALVAVRLR
                     Y"
     gene            44955..45323
                     /locus_tag="PKV56_02685"
     CDS             44955..45323
                     /locus_tag="PKV56_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66745.1"
                     /translation="MRRVLSILLVLAAAAPFPALAAATVWLCGLSEDLVRLVCIADTG
                     PQSPEAAPEAAATVNGVQFPLDPRRTYVVDLWSPPTEVAFVEQLARATICYRSPGCEV
                     LLVSPLPAPAASDGRQRRSR"
     gene            45378..45992
                     /locus_tag="PKV56_02690"
     CDS             45378..45992
                     /locus_tag="PKV56_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX66746.1"
                     /translation="MRSALQGAPRPGFLGTPADPATGRRSAVAACALWVSLNLCPLLA
                     AGQPAAAPPGEVQAGQVLPEALLPGLNGPSRKLSGYRGRPLIINVWASWCGPCRQEMA
                     SLERLAWQPNDPSFAVIGISTDDDPDSARRLLQATNATLSHFIDDRLRLEHMLGASRL
                     PLTVLVDAQGRVVAKVYGAKQWDGPEARRLIDQAFGNRPSPARR"
     gene            46174..47304
                     /locus_tag="PKV56_02695"
     CDS             46174..47304
                     /locus_tag="PKV56_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX66747.1"
                     /translation="MRVTQGSRKLLMAAVVAAGSLNAQAGARYDFGEDRNVTFGIGTR
                     YSYTHAERPDSAGGGHVNDPNLDSARLFFGASLSKNIKATFNTEWDGVENRVRVLDIY
                     AQFEFLPEINVWAGRLVSPSDRHNMAGPYYSMGGGYWPCVASRYGCNGGIFRARDDGF
                     VVWGNVADSRLGYSIGAFDGRTFGIGALDESQARGAGVKSSGSLMYAGRLQYDFWEKE
                     TGYFSAANHLGKQDILAIGVAGRYQKKGVLTTGGRGDYWSYNVDFLFEKSIKGSGGVA
                     VEAAYYDYDTDDVVRAEQGKAYSAGVSYIFEQPVGWGQLQPFTHWQKFNPDSNTDTRQ
                     IDVGVNYVIDGFNAQLTAMYTNTKVTSSKSLDKFVLALQLQY"
     gene            complement(47642..48502)
                     /gene="purU"
                     /locus_tag="PKV56_02700"
     CDS             complement(47642..48502)
                     /gene="purU"
                     /locus_tag="PKV56_02700"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOX66748.1"
                     /translation="MNRTHHYTLTFSCPDRVGIVARVTGFFAEHGGWILSSSQHADAD
                     SARYFMRVEVRADSLPFDHAELKRRFQVVATEFGMDWRISDSAVRRRVLMLVSTQEHC
                     LYDLLARWKSQELDVDIVGVASNHEVFRGLVEWHGLPFHHIPVTKENKPEAFAKLEAL
                     FESSGADTMVLARYMQILPPSLCRKYPGQIINIHHSFLPSFAGAKPYHQAWDRGVKLI
                     GATCHYVTEDLDEGPIIEQDVMRIDHSDAVEDLVRCGKDVEKAVLARGLRNHLEDRIL
                     INGHRTIVFG"
     gene            48646..52182
                     /locus_tag="PKV56_02705"
     CDS             48646..52182
                     /locus_tag="PKV56_02705"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOX66749.1"
                     /translation="MNHRPIRSILIANRSEIAIRVMRAASEMRIRTVAIYSQQDRQAL
                     HRFKADESYLVGEGKKPLAAYLDGEDILRIARSAGADAIHPGYGFLSENPEFADAVIA
                     AGIRWIGPSPDVMRTLGNKVAARHAAVAAGVAVMPATPPLPLDVAECARLAAGIGYPV
                     MLKASWGGGGRGMRVIENEGELAGSLEASRREALAAFGNDEVYFEKLIRRARHVEVQI
                     LGDQHGNLVHLHERDCTVQRRNQKVVERAPAPYLDAAGRAALCDSALRLMRSVGYTHA
                     GTVEFLMDADDGRCYFIEVNPRIQVEHTVTEMITGVDIVKAQIRITEGGHIGVTEGDQ
                     VGSPEGSPEEASGLHPTGVPPQDKIPLNGHALQCRVTTEDPENGFLPDYGRLAAYRSA
                     AGFGIRLDAGTAYGGAVITPYYDSLLVKVTAWAPTARETIQRMDRALREFRIRGVSTN
                     LQFLENLINHPTFGNDVNGELTTRFIENTPELLAFAARRDRASKLLRFLGEVAVNGNP
                     EVQGRTLPALPLPKPVLPKVDLESPIPDGTRQRLQALGPAGFAQWMLAHKPALITDTT
                     MRDAHQSLLATRMRTADMLPIAPYYARELSGLFSLECWGGATFDVALRFLKEDPWQRL
                     ALLRQAIPNVLFQMLLRGSNAVGYTNYADNVVRHFVKQAATNGVDVFRVFDSLNWVEN
                     MRVAIDAVIEYGGLCEGAICYTGDLYDSARGKYDLAYYLGIARELQKAGVHLLAIKDM
                     AGICRPRAVAALVKALKAETGLPVHFHTHDTSGASAASVLAAIEAGCDAVDGALDAMS
                     GLTSQPNLSSIAAALAGTERDPGLPQDALHAASMYWEGVRRFYSPFESEIRSGTADVY
                     RHEMPGGQYTNLREQARSLGIAHRWTEVSQTYADVNRLFGDIVKVTPTSKVVGDMALM
                     MVASDLTPADVMDPAREVAFPESVVSLFKGELGFPPDGFPAALSRKVLKLGEGDQPPV
                     PYRPGDRLSPVDLEAVRARAEAECELPMDDSQLASYLMYPKVMRDYCEHTRLNGDTSM
                     LPTPVFFYGAQPQQELAIEIDPGKTLLVALQSISPDGDVAQKVQFELNGQSRTVRVLR
                     PDAGDAAKGRPVADPTNPLHVAAPMPGAIVSVAVKPGQRVSAGSTLLALEAMKMETHV
                     AAERDAEVEAVLVVPGDRVQAKDLLILLKAVS"
     gene            complement(52202..>53429)
                     /locus_tag="PKV56_02710"
     CDS             complement(52202..>53429)
                     /locus_tag="PKV56_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOX66750.1"
                     /translation="LAGTGALYLFHHEIDAWWHRDIQRVESAGASPRTHQASVDTAVA
                     SQGGQVFRYVPPATASAAAEVDILAPTGEKIAVYVDPYRARVTGVLPDRGTLMWTVRK
                     LHSLKYFGPVASGLVEIAGGWCILLVATGLYLWWPRGQAGGVSTVRGSVQQRVFWRDL
                     HAITGSIAAAFIVFLAVTGMPWSVLWGSKVNEWANGHNFGYPAGVRVAVPMSDEHLAH
                     SAPAAWSLQQARVPQSALSAHTPISLNEAITRFEALGISPGYAVTPPAGATGAYSASV
                     YPSDLTRQRVIHLDQYTGKPLIDMSYADYGPLGRSLEWSINVHLGQQYGEVNRWLMVF
                     ACAAIVLLCVSATVMWWKRRPSGSLGVPPQPADTRKSWHVLAMLAVGGIVFPLVGASM
                     LLIALADTLWTRRTRI"
BASE COUNT         8882 a        17729 c        18061 g         8757 t
ORIGIN      
        1 gctgaccgag gcggccgaga ccatgcgccg gggcggtggc gtgggctacg acttctcccg
       61 catccggccc agcggcgcct gggtcggcag cacgcgcagc agcgcgtcgg gcccggtcag
      121 ctacatgcgg gtgttcgacc ggtcctgcga gacggtcgag tcggccggtg cgaggcgggg
      181 tgcgcagatg ggcgtgctgc gctgtgatca cccggacatc gaggccttca tccgtgcgaa
      241 ggacggcggc gacctgaaga acttcaacct gtcggtgggc gtcaccgatg cgttcatgca
      301 ggcggtgcag gccgacggcg aggtcgaact cgtgcaccgc gccgagcccg gcgtggcaca
      361 gaaggaggcc ggcgcctacc agcaggcgca gcccggcggc ggtgggctgt gggtgtatcg
      421 caagcgtcgc gcgcgcgaac tgtgggacct gatcatgcgg tcgacctacg accatgccga
      481 gcccggcgtg ctcttcctcg accacatcaa ccgcgacaac aacctgtcgt actgcgagac
      541 catcgccagc accaacccct gcgccgagca gccgctgccg ccctacggct gctgctgcct
      601 cggctcgatc gacctgaccc ggttcgtgcg cgagcctttc gaggatcagg cccgcttcga
      661 tgaagcctcg ttcatctcgg tggcgcaggt ggcggtgcgc atgctcgaca acgtgctcga
      721 tgtgacggtc tggccgctgc ctcagcagca ggaagaggcc cggcacaagc gccgcgtcgg
      781 cctgggcttc accggcctgg gtgatgccct ggtgatgctg aacctgcgct acgacaccga
      841 gccggcgcgc gcgatggcgc ggcgcatttc cgagctgatg cgcgacgccg cctacgaggc
      901 atcggtcgag ctggcgcggg agcggggttc gtttccgctg ttcaaccccg acctgtacct
      961 gagcggaacg acgttcgcgt cgcgcctgcc gcagggcctg cgcgaccgca tccgcagcca
     1021 cggcctgcgc aattcgcacc tgctgtcgat cgccccgaca ggcacgatca gcctggcttt
     1081 cgccgacaac gcgagcaacg gcatcgagcc ggccttcagc tggacctaca cgcgcaagaa
     1141 gcgcatgccc gacggcaact tcaaggagta cgcggtcgag gaccacgcct ggcgcctgta
     1201 ccgccacctc aagggcgaga aggcgccgct gggcaacgcc ttcgtcaccg cgctggagat
     1261 gagcgcgaag gcgcacgagg cgatggtcgc ggcggtcgcg ccctgcatcg acacggcgat
     1321 cagcaagacc gtcaacgtgc cggccgacta cccctacgag gagttccagc atctgtacct
     1381 cgaggcctgg cagtcggggc tgaaggggct ggccacctac cggccgaact cggtgctcgg
     1441 ctcggtgctc agcgtcacgc ccgcggccgc cgcgccgctg cagatcgacg acaagaaccg
     1501 ccgcctcgcg ctggagcgcc tgccggcgcc ggtgctgtcg tcgctgcgct ggccgggccg
     1561 gcccgagctg cccggcggca acccggcgtg gaccttcatg gtcaaccacc cgttcggcag
     1621 cttcgcgttg ttcgtcggtg aactcgcgcc cgagagcggc ggtgcgccgc agcccttcga
     1681 ggtgtgggtc aacggcgccg agcagccgcg cggcctgggt gcgatggcca agaccttgtc
     1741 gatggacctg cgcgccaacg acccggcctg gctgcagctc aagctcgacg cgctggccac
     1801 ggtggccgag gagcgtgcct tcgagatgcc cttcccgccg catggcgaga agcgcttgtt
     1861 ccccggcgtg gtggccgcca ccgcggccgt ggtgcgctgg cgctgcgagc agctcaaggc
     1921 gctgccgcgc ctgggccaca aggcgcccac gccggtgatc gacgcgatgt tcagccgcga
     1981 cgagccgcac accggcccgt cgggcacgct ggcctgggcg gtcgacatcg acaacccggc
     2041 caccggcgag gccttcacgt tgacgctcaa ggaagtgacg ctgcccggcc ccgatggcgg
     2101 caccgtcacg cggccctgtg ccgtcggctt cagcggcaac tacccgcgtg cgctcgacgg
     2161 cctggcgcgg ctgctgtcgc tggacatgcg cgtcatcgac ccggcctgga tcggcatgaa
     2221 gctgcgcaag ctgctcaact acgccgagcc gctggggcac ttcatggcct tcgtgccggg
     2281 actgccccac ggcgaacgcc gccagcagac ctggccgtcg accgtggcct acctggcgcg
     2341 cctgatcatc catcgctacg cgatgctcgg cgtgctcgat gaggagggct atccgttgcg
     2401 cgacatgggc gtgctcgagt cgtccaagcc ccgtggcgag cgcgacccgg tgatggcggg
     2461 caaggtctgc ctcgagtgcg gcaatgcgac cgtgatccac aaggacggct gcgatttctg
     2521 caccgcctgc ggagcggtcg ggcagtgcgg ttgaggtcct gatcagcacg gcgcgcctcg
     2581 tgtgcgcagc ggctgccccg aagggattta aaggacattt aaatgtcccc gtcgagagaa
     2641 tcgtctggac atggcgtgtt cagaagattc tctttttcta cctgcgtaca gacagacggc
     2701 tcccggttcc gcacaggcgg aaccgaccag cacaggtgat ccggcggccg atctcgagga
     2761 ttcgaggtcg gtcccgttcc atctcgtcaa gacctcgatc agccgatgcg agcggcttcg
     2821 acagaccctc gtcaccgcgg tgttcgtccc gtctgcaatc gaacatcctc gaaccatcct
     2881 ggggtgtgtg cgcatcggag gtgtgtctcc gatggagttg cactcggtcc gcatcgggag
     2941 cgagcatcga acggtcttcg agatgaagac cctggccgac gagatgggcc gctcgatctc
     3001 ggcgggcgaa ctgctggaag ccgtcttcgg ccagcgcatc ccgcaggggc tctgcatttc
     3061 agcccgtcag ttccaggggc cggtggccac ctttcaggcg ttcatcgagc tccaggtgag
     3121 cgcttgcgaa tcgagcccgg ccagcggcct caaggtgtcc tgcggcgtga actgacttcg
     3181 gtgtccgggc gactcgtggg cgagccggtc ggcctgcgtt gcgcggacgt cactgcgggt
     3241 agccgggtcc gcgcgagtgc cacctcaggt gcgaccggga cgcatcatcc gatcgggcgc
     3301 gtccggtgga atctttcagt ggcgtgacgg cgtggacatg gcgatcgata cccgcaatgg
     3361 cttcggccgc agtcgtcagg cagttcaacg cgccggaatc cccgtcggtc atgcgagtcc
     3421 actcatggac gctggttcgc agggacgcat cgccctcgga caccaccatc agaccgccga
     3481 cgcgcctcat gcgaccgatg gcctgctcca gccgggccag atcggtggcg tgctgcacct
     3541 cgattcgcca gatcacgcca ctgcccaggc gcaggtaggc tttgcagatg gcggagcaca
     3601 gcatctgtcg tgtcgaagcg tccgcgccga ccagcgcgag ttgtcccagc ttcttcaatc
     3661 ggcgcgccag gctttgcgcc atcagctcgc cgggatccga cccgatggtg tccgacctgt
     3721 tcggctgcgg ctgcggggcg cggctggccg agcccacgac cgcgaattcg atggcgtcgg
     3781 tcagcatgta tccctgtctg gggaccgtgc tgatggcatc ctgcccgaga cgctggcgca
     3841 gagcccagat ctggacatcc aggttgttgg gcgtcacaca aacgcccggc catatcgcgt
     3901 catgaaggta ctgcttgggc accacgtttc cgcgcttttc gagcagggtc cagagcagat
     3961 cgaaggcccg accaccgagg cgaacttccg ttccgtccaa cagcaagcga cgcgtttcgg
     4021 gcagcacctg acagcgcctc ggacgcgttg tcgtgctgcc agcgaagagg cctgaatcat
     4081 cgtgtccgct cataggggat caagtatgca gccggccggt cacgcggccc accgctgacg
     4141 ccggagtcgg cggttcaggc cggtacggtg tagctggcct gggtgacgag gtccacaccg
     4201 cagctcaggt tctcgatcca ttcgatgaat tcctgaaccg ccggtcccac cagcggtgcg
     4261 ccgtgctgcg gcacgatcat gtcgatgggc agggtgcgcg ccatctgggc ccacagccgc
     4321 agcaccttgc ccgacaccat gtagcggcgg tggaagcctt ccatgcgctg gacgtgcgcg
     4381 gccagcgagg tgatgggtct ggttgcgtcg gccgaagtgc cgagcgacac gccgaggtct
     4441 ccggagaaca ggatgcggct caccggatcc cagaactgga agttgccctc ggagtgcatg
     4501 aagtgcgcgg gcagggcgat cagttcgctg cggcccaggg ggatgcgcat gcccgcgtcg
     4561 ggtatgccgg tgatgcggcc ctgtgtcttg ccaggcttgc agaagtgcgg cgcaaagcgt
     4621 tcccacaggc tggagatgta gagcgtggcc tgggtgctgg tcatccagcg gtcgagcgac
     4681 gcgatgatgt ccgggtcggc gtgcgaagcc aggatcttgg aaagcttgct cggcgggaag
     4741 aatcggccga tggtcagcag caactcgttg taggccacgt tgccgcccgg gtcgatgatg
     4801 gcgccggtgt cgccgtggat caccaggaac tggttggcct gcacggcctc gccgccctcg
     4861 tcgctcaggt gcgcgaacat caggcacaca tggccgttct ggttgtagag ctcgatggcc
     4921 atggggtgtt ctccaacgat ggaatgccgc ggaccgcctg caactgcgcc gtctggccgg
     4981 cttgccctga gcgcgacttt agggatgaag cagccgacgc cggttgaggc atgtcaagcg
     5041 ggcctgcacc cagcgtgaat atttcgtcca atgggcgttg cggtgtggtc cggaaatcga
     5101 caggaggcag ccaggctctc gtggctgcac gcccgtcgtg tcgcactgcc agcgattggc
     5161 tggcaaatca atttaagaaa tttaaagcgc catccggcgg ccaaggcacc gcgctgcgcc
     5221 cgcggccggc gagcccaccc gtgacaattc cgcggatgac aaacaccgtt accgagagct
     5281 tcttgcagga agaaggatgg ctgcaattgc gccagatggt cgatgcgtcg gcgctggtgc
     5341 aggtcaaggc gctggtcgac cagcacgccg atgacttcgc ggatgatttc tacgaccgca
     5401 tgctggccga tccggccatg ggacgattcc tggaccacga aatcgtcaac cgccggctgc
     5461 atgcgtcgat ggtccggtgg ctcaggttcg tctttgatca cgagtcgccc gtctctcagc
     5521 tccaggaaat gcagcgcagg accggcgaag tgcacgcgcg gatcggcgtt ccgcacgccg
     5581 ccgtggctca aggggctcga gtgctcaagc gggccttgtc cgcacgcgtg ctggcggatg
     5641 cacgcttgcc ggccctcagc gccgaagcgg tgcagtacgt ctatgaactt ttcgatcttg
     5701 cgatggacgt catgggcgag agcgcgagtg ccgacgaggc acgacttctg cgctccgacg
     5761 agtcctaccg gctgttcttc ctgagccagg acctgcgcgc agagcgcgag cgccagaaat
     5821 cgcagttgct cgagtgggcg aaccagatac tggtcaacaa ctactgggat gtacagcagg
     5881 agaacgacgg ggacgccgat ccgggtggca ccgtgtcgca gttcaagctg tgggtcgaac
     5941 acaaggcgct gatgttgttc gagggggttc cggaaacggc gctgctccgg gagtgcatcg
     6001 acgcgaccga ggcagagctc ctgcccaggc tgaaagcggc tcgggaccat cacgccagcg
     6061 cgaaggaagt cgtctccgaa ctgaatgcgg ccgtcgagcg catgaaaggg ctgctgggcg
     6121 cgatgttcga ccatgcggcc cgcctggagg atgggcgaga cgagctgacg cggctgctga
     6181 accggcgcta ctttccttcc atcgtgaagc gcgagatcga actggctcag gtgggcggac
     6241 acccgtttgc gctgctgctt ctggacctgg accgattcgc cgacatcggt catgccctcg
     6301 gctccgacgc ggtggacgcc gtcatcgcgc agatcgccga caagctgagt ggcagcgttc
     6361 gagcgggcga cttcgtgttc cgggtctcgg aatgccggtt tctcgttctg ctcgtcgagg
     6421 cgaacgatcg cgaggccatg accgtggccg aagaactgcg ggtccgcatc gagcacctgc
     6481 cattgcgcac gcctgcaggg gtcgctccgc aggtgaccgt cagcatcggt gtcgccttct
     6541 tcgacgggca tccggactat cagcaattgc tcgaccgggc ggagcgcgca gttcgcgagg
     6601 ctcggtcctt tggtggcaac cgggtggagc gggctgaacc ctccagcccg tccgcctgcc
     6661 cgttcgccag gagccagttc ccgaaggcct gacatttgtg tccatgcacg gcatcgacaa
     6721 ggtgctgccc ccgcggccgg ccgaggtccg attcgggcag aaagaaatgc gggtgacggc
     6781 aatccgggcg tcgaaaagga gatcaccatc agcgtctcct ggctgcggca ttcacagcag
     6841 tcattcaacc atcttcagcc tgccggcctg cgccggtggc ggctgcctcc aaccagcccg
     6901 tcgcgcgcgc catgggcgcg cggctccgac gcgacggctc gaccggcacc ttccgaccat
     6961 gacccgaatc aagctgtgtc tcgcaggcct catcggactg ttgagtgccc tctggctgct
     7021 ggccgacacg ctgctgcctc agcctttcac gtacttctcg ttccgctcgg tgttcatgca
     7081 gtacagcggc gtactcaccg tgggtgtgat gagcgtcgcg atgctgctgg cgctgcggcc
     7141 gcggtggctg gagcgcccgc tcgatggcct ggacaagatg taccggctgc acaagtggct
     7201 gggaatcacc gcgctggtcc tgtcggtgct tcactggtgg tgggcccagg gcaccaagtg
     7261 gatggtcggc tggggctggc tggtccgccc ggcccgtggg ccacgccccg atcgctcgca
     7321 actcgacacg gtcgaggcct ggatgagcgg tcagcgcgat ctggcggaat cggtcggcga
     7381 atgggctttc tacgccctgc tggtcctgct ggtgctggcg ctggtcaagc gcttcccgta
     7441 tcacctgttc gtgaagaccc acaagctgat cgcgctggcc tacctggtgc tcgtctatca
     7501 ctccgtggtg ctcgccaggt tcagctactg gagcgagccg atcggctggg cgctggctgc
     7561 cctgatggcc tcgggaggtg tcgcggcggt gctggtgctc ctgggtcggg tcggtgccgg
     7621 tcgcaaggtt cagggttcga tcgagtcgct ggtccactat cccggactcg acgtgatcga
     7681 gtcgagcgtc gcactcgggc cgggctggcc cggtcacacc gcagggcagt tcgcgttcgt
     7741 gacttccgat cgccgggaag gtgctcaccc gtacacgatc gcctcggcct ggaatgcgca
     7801 ggacggtcgc atcaccttca tcaccaaggc actgggcgat cacacccgac gtttgcgcga
     7861 gcacctgaag gtgggcatgc cggtgacggt cgagggacct tacggctgct tcgatttcga
     7921 ggacgaccgg ccccgccaga tctggatcgg cgcgggcatc ggaatcaccc cgttcgtggc
     7981 ccgcatgaag catcttcggg acaggccggg ccgggcatcc atcgacctgt tccacccgac
     8041 cgcggttcat gaacccgccg ccatcgccaa gctccatgcc gacgcacgcg cggcgggtgt
     8101 cgccgtgcac ctgctcgtgg atgccaggga cggtcggctg gacggcgagc gcatacgcgc
     8161 ctcggtgccc gactgggctg acgcgagcat ctggttctgt ggaccgccga gcttcggacg
     8221 cgcgctgcgc aaggacttcg tggcccgggg cctgccgacc gggcgcttcc accaggagat
     8281 gttcgagatg cgttgagcat tcgaaggcga atcatgaaat ctgcctttcc cggattcgac
     8341 tcccccgctg tcgggttcga gcagccgttc gagatgctgc tggcgtgtca cgagcgggtg
     8401 cggcgcagcc tctcgctgct gggccgcctg gtcagtcgtg tcggcgagcg cggacacgac
     8461 gatgccacgc gctccgcggc gtcggatgtg ctgcgctatt tcgatctcgc cgcaccgctg
     8521 catcacgagg acgaggagac gcatgtcttc ccggtgctgg ctgccagcgt ggacgccgag
     8581 ctggttgcgg cggcctccag cttgcaggcg gatcatctgc gaatgaccgc cctgtggtcc
     8641 gccattcgcc cgacgctcga agcatggtcc gccgcgcacg cgagcggcac tgccgaagcc
     8701 gatttcctgg atcgggccgc cgacttcgaa cggatctacg aggcgcatct ggcgatggaa
     8761 gagcggctgg tgttccctgc cgcgcgcgag cgcatcagcg cgagccttct ggccgccatg
     8821 tcgagcgaca tggctcgacg gcgaggggcc cctgtcggcc cgcgttgagc ggcgggcgca
     8881 tcgggccagc cccgactcga ctcagcccgc ggtcgtcgtg ccgggcgtga gcaggcgctg
     8941 catcagctcg cgcacgctgg ggatctcgtt gccgaatgga agccgaccga cgccccggaa
     9001 gaagagtcct ttcttgacgt cgcctcgcat ggccgccgcg agttgcgtgt cgatgcagaa
     9061 ctgcccccaa cctggcaggg catcgcgcag gccgcactgc ttcaggcagt cgaatgccag
     9121 cgtgcagcgt ggcttcggat gcgccacttc ctggagtcgt tgctcggcct tcaggtagct
     9181 ctgcaaccac ggcgttgaaa cggcgcgggc cggcaggccg gccgcgctgg tgaactcgat
     9241 caggtcctcg tcgttcgcgt tggcgagcac gcgcttgaat tcgatgtgcg cgtcgcactc
     9301 cgtcgtgact gcgaacggtg tgccgagctg gacgcccgct gccccgagcg actgcagccg
     9361 cgcgatgtcg tcgaagcagc gaatgccgcc ggccgtgatg atcggaatct ccttctcgat
     9421 gccggctgaa cgcaggaatt cgcgtatctg cgggatggcc acgtcgaagt cgaaatgcgg
     9481 actggtcagg tcgctcaccc tggccgcacc gaggtgtccg ccggccaggc cggggtgctc
     9541 gatcacgatg gcatcgggca accgcttctt gcgctcccac ttgcgcacca gcaactgaac
     9601 gccgcgggcg tcggacagga tcggtatcag cagcgcgcga gggtggtcga ccgcgaggtc
     9661 gggcaggtcg agcggcagac cggctccgac gaccagcgcg tcgatgccgc attcgagcgc
     9721 gcgcgtggcc gacggcccgt attcgttgac cgctcgcatc acgttgatgg ccagcaggcc
     9781 tcgtccttcc gacagagcct gcgctgcgcg gatctcgcgt tccagcgcga cgaggttggc
     9841 gtcgtcgatg gcccgcttgg ccgctgcggt tccggccggc ccgggggcga tgccgcgcgt
     9901 gagttccatc aggtcgggat ggtgacgacg caggtcgatc gacgagatcg tgccgaccgc
     9961 gccgagcccg gcgaccgtgc ccgccagcct gtgagccgac acgccgactc ccatgccgcc
    10021 ctggacgatg ggaagcagcg agcgtccgcc gaggaccagc ggcttgagcc cgctgcgcct
    10081 cagcacctgg gtcaggtcgt gggcctcggc gacccgctct tcagtcgaga tcatggtgtc
    10141 ggcacgccgc ccgcagggct gcccggttga atgttcggtt tcacgaggta tctcccattt
    10201 cgaatgtcga tgaccgtttc agtggcgcct tcaggcgcct gcatcgcgtc ggggatcccc
    10261 gagcagttcg agcgcgcgct gctcgtcggc agccgacagc gccggcggct ccggcgtgcc
    10321 gggtctgcgt ccgagcgacc gccacagcgc gatcaacccg agcagcacga gcagggcggg
    10381 tcctgcccag agcagcacgg tgctcgcctt cagtggcggg cggtacagca cgaagtcgcc
    10441 gtagcgttcg gtcatgtagt cgatgacctg ctgctcgctt cgaccggcgc gcagctgttc
    10501 ccggatctgg ttcttcaggt cgaccgccag ctccgcgtgc gaatcggcga gcgactggtt
    10561 ctggcacacg aggcagcgca gttcggcgcc cagcgtgttc acacgcgact cgagagcagg
    10621 gtcggcggcc actggcacgg ccacgcctgc accggccagc gtacagacgg ccaacagcag
    10681 gatcgcgacc agcctggctg cgcagtgcgt caccggtcaa ctccgaagct gctgcacgat
    10741 cggcagcaga tcacgggtcc agacttcccg tgtcagtggc cccacgtgct tgaagcgaat
    10801 gacgccgctg cggtcgatga cgaaggtttc cggaacgcca tacacgccgt aatcgatgcc
    10861 cacctttcca tcgcggtcga gtaccgactc cgcgtagggg ttgccgagtc gctggatcca
    10921 ctcctgtgct gcgcggggat cgtccttgta gttcagcccg tagatcggaa cgatctgctc
    10981 gcgcgcgagt gcgacgagaa aagggtgctc ctcacggcac ggcgtgcacc aggacgccca
    11041 gacgttcagc agccagacct tccccttcag gtcggcgctg gccagtgcac gctgcgattc
    11101 gctcagcagc ggcaggctga acgccggtgc ggccttgccg atcaggggcg aaggaatttc
    11161 ccggggcttc agccgcagcc ctgcgcccag caacgcaccg agcaggatga acgcacacag
    11221 cgggaccagc agccgggggc tcaaggcgtc gccccctgcg cgtcgatgcg ctgcgccgca
    11281 ccggccagtc cggattcggc cgattcggac tgaacccggg acacccggta tcgccgatcg
    11341 gtcgcggcga gcagtccgcc cagggccatg acgagcgcgc cgccccagat ccagtcgacg
    11401 aacggcttga tgtacacgcg cagggtccag gtcttgtcgt ccagcggctc gccgagcgag
    11461 atgtagacat cacgaagaaa gccggtgtcg atggcggcct cggtcatcgg catctgctgg
    11521 accttgtaga gccgcttctc gggcttcatc gagaaggtgt ctccgccgtc gcgctgcacg
    11581 tcgatgtcag cctgcacggc cacgtagttg gggccggtgg actcgctgac gcccttgaac
    11641 cggatgctgt agccgccgat gtcgaggtga tcgccggtgc tcatcttcac gtcgctctcg
    11701 acatcgaaac cgcgcaccat ggtcacgccg acgatgaaga tgcccacgcc cagatgagcc
    11761 accaccatgc cgaagaagct gcgtgacgaa cgaccgagct gcttccatcg ttcgcccaag
    11821 gtgttcccgc gcagtctcgc gagcagcagc gcagcggtgc tggtgaacac ccatgcggcg
    11881 aggaagagcc ccagcgcgac gccggcactg aaccgtccgg cccaccaggg aagcaccagc
    11941 gcagccgcca cgctggccat cgcgggccac cgcaggcgag acgccatgtc ggcccacgaa
    12001 gccgacttcc agcgcgccag gggaccgacg cccatcagca ggacgacggg agccatcagc
    12061 ggcacgaaca ccgcctcgaa gtacggcgtg ccgaccgaaa tcttgccgag gcccagcgca
    12121 tccagtgcca gcggatacag cgtgcccagc atcaccgagc cggtcgcggc caccagaagc
    12181 acgttgttca ccagcagcag cgactctcgc gacacgcgat cgaaacgccc gctgaagccc
    12241 acgctggcgg cgcgccaggc gaacaacgac agcgagccgc cgatcacgat gccgaggaag
    12301 gacaggatga acacgccgcg ggccgggtcg gttgcaaacg cgtgcaccga cgtcaacacg
    12361 ccggagcgga ccaggaacgt gcccagcagg ctcaggctga acgctgctat cgccaggaga
    12421 atggtccacg ccttgaaggc gcctcgcttc tcggccacgg ccagcgaatg gagcagggcc
    12481 gtgcctgcga gccagggcat gaatgacgcg ttttccaccg ggtcccagaa ccaccagccg
    12541 ccccagccga gttcgtagta ggcccaccag ctgcccagcg cgatgcccac ggtgaggaac
    12601 atccatgctg ccgtggtcca gggtctcgac cagcgtgccc aggcggcatc gaagcggccc
    12661 gagagcaggg cggccacggc aaacgcgaag gcgaccgcga agccgacata gcccatgtag
    12721 agcatcggcg ggtggatcac catgccgggg tcctgcaaaa gcggattcag gtcgcgcccg
    12781 tcgagtgccg gcgggttcat gcgctcgaat ggattcgagg tgagcaggat gaacaacagg
    12841 aagccgacgc tgacgaaacc catgacgccc agaacgcggg agaccatctg cagcggcagc
    12901 cggcgcgaca gcaggctgac cgccagcgtc cagcccgaca gcatgaacac ccacagcaac
    12961 atcgagcctt catggctgcc ccacgtcgct gcgatgcggt acgccatcgg cagctcggag
    13021 ttggagttgg aggccacatt gacgaccgag aaatcgttgt tcgcgaacga gaccatcagg
    13081 ctggcgaacc cgatggcgat gaaggcgaac tgcccttgcg ccgcgggccg tgccagcccc
    13141 atccaggcaa tgtcgttgcg cgcggcgccg atgagcggca gcgtcgcgag gaccacgccg
    13201 aggaacagcg caacgatcag cgcgaaatgg cccagttcgg gaagcatgcc tgtgtctcag
    13261 ttccaggtgc tggacgcatc gctgcgcgaa cggcgttcgc cttcgcggct ctgcgcgcga
    13321 ttgatcagcg cgtcttcgcg gatttcatcc tcggcacgtc gacgtcgttg catcagcatc
    13381 acgacctccc aggccagcgt ggccgcggcc atcaggtacg agccccagac gtacagaccg
    13441 tagccgccca tctccgcgaa ttcggcgacg ctgtgccagt tcatcgccgg agctccagct
    13501 gctgcctgac ccagtcggca tggcgctcgc tctccaggat gatcgaccgc acgcgatgca
    13561 gcgcggcggc gaaggaccag gcccagacgg ctgccgtcat cagcagcatg cccgtgacgc
    13621 tccaggaagc cccatgcagc ggagcgctcc accacgggtc cgagaaatgg gttgcaggca
    13681 cggccagccc gccgcccagg accagcaggg ttgaagcgcg ctgcatgcgc cgacgatcgt
    13741 cgatggcgga ctgcagggcc atgaacccga tgtacaggag gatcagcagc agctccgaga
    13801 tcagccgggc atcccagacc caccaggtgc cccaggtcgg cttgtcccag agggcgccgg
    13861 tccacagggc aatgaaggtc atcaacagtc cggtgggcgc cagtgccgag gccatcatcg
    13921 acgccatgcg cgaattcagc accagcgcga gcgcgcacca gaacgccatc gacaggtaga
    13981 tcagcagcga aagtcctgca gcaggcaggt ggaccaggat gatccgatcg gcatcgccct
    14041 gctgaccctc ggccggagcg atgagcagac ccaccgacag tcctgccgct gcgaggagcg
    14101 cggagatcgc ccaggcccag cccatcagtc gcccggcgag cggatacagc tgctgaggcg
    14161 acgcatggcg ctcccagcgt gaccgggtgc tctctccttc gcctgacggg gtgcggtgca
    14221 gggccatctg gacgctcatg ccgtgccgga gtgcaatcgc cgtgccgcgt gcgcacggca
    14281 cgatgggccg gtgtccggca gcgtttcttc ccgatctcgg gaatccctgc aggcgagtcc
    14341 gggaatcgct gcgccgctca tcttgcggcc cgcgcttcgt cgcgtcgaaa gagggcacgc
    14401 gccgcgtagg ccgcgatgtc gtcgatttcc cgtgacgtca ataccgagcg ccaggcgggc
    14461 atggacgtgc ctggcaggcc gtcccggatc acctggcgca ggcgtgcccg cggcatcgac
    14521 tgcgtggagt acgcggtgag gtcgcgcgcc ttcggctcca tgaactggcc gatccagttc
    14581 ttgccgctgc catcggcgcc gtggcagaac gcgcagttgg cctggaacag cgcctcgccg
    14641 cgacgctccg tggcgcccag tccttcgatg cgcggtgcga cctcgtgctt ggcatagacg
    14701 ctcgaggtcg acacggcatc gacgggaggc gtgttcggct gtcccggcac gaatcccatg
    14761 cgtggaaacg acagagggcg cgcgctccat gcaggcgcgg agtcgtcctc acgggggccg
    14821 tcgtggcaag agatgcagcg ggtcatgaac agctcccgac cgcgccgctg ggtggcgtcc
    14881 agcgattcga ccggcgcact gatcgagatc tggccccgtg cgtacgggaa ggcggctgag
    14941 aagcgctcgt gcccgggcca tccgttcgcc tcggtgtgat aggccgtgtt ggcggccttg
    15001 tcccgcacga actcccgttc gacaaaggcg gacacggcat cgatttccga ctcggtgagg
    15061 atgccgttga acgacttcat ggctgttccg tcgcgaccga gtcgcaccgc ccgggcaatg
    15121 gtgctgcgtg gcagcgaggc gatgtcggcc ttcgtgaaat cccgcggagc cggggtcaga
    15181 tagttggacg ccagcgtgcg tgcgtcgccc gagtacccat ggcagaagta gcagcggaag
    15241 ttgtagacct tgcgcccgag ttcgtgctga tcgctcggag ccgcagtgct tgtctcgggc
    15301 gacggcgctg cgcgcgcgct gggcaggctg cccgttgcgt cgcagccgcc gaggagaacc
    15361 agcacgctga tcgcggcgct gagcacggcc ccgaaccgca tcgcgatcag cgttgtgccg
    15421 gcgaaccggc cacttcggtg gccttgccct ggacgaagct ctggaacacg tactcgctga
    15481 cgtcggcgat ctgttgcggc gtggcgacct gcttccaggc cggcatctcg ctgccgaggc
    15541 ggccttcgct caccgcggca tacaaggcca cccggttgaa acgcgcacgc gaggcgtctt
    15601 cggtgaagtt gcgcggcttc ggattgatga agtaggcgcg aggtcccgcg ccgtcaccgc
    15661 gcgcgccatg gcaggtggcg caattggcaa ggtagaaggc gccgccccgc ttgcggttgc
    15721 ccttgagacc ctcgggaaac tcgacgttca tgttgacaag cgatggggcg acctgcgacg
    15781 cgacggcctg atcggcatcc cgccccccat gcgcccgggt tcccgagatg gcggtgcctg
    15841 acatcagcgc tgcgcggatg tagtcgacga cctccgcgat gtcgctttcg ccgaggcggc
    15901 ccgcgaagcc gaccatcggt gtgcccggct tgccgtgggt cacggcggcg agcatggcct
    15961 cacgggtgag gttctgagac gcgggagcgg tgaagtcgcg cggaggccgg gccatgttgt
    16021 cgcgtgccca catggagcct gctccctggt ctccatggca caccgagcaa ttgcgttcgt
    16081 agatctgacg gcccttgctg gcagcctgcg gggcgggttg aacgaagcgt gtgaacacgt
    16141 gatctgccag gcgcgcaatg tcgtcggccg acaactgcct gttccaggcc accatggctg
    16201 tgccgggctt gccgtgggtg atcgaggcga cgatgcgttc gcgagtgagt tcctgcttcg
    16261 acgccgcaga cgtgaagtcg cgaggcggcg tcgacagcga accggacgcg cggctcttgc
    16321 cgtcgccctt gtcgccgtga cacaccgagc agtagttgtg gaacagcgtg gcagcgcggt
    16381 tcgacggttc ttcggcgcct ggagccgacc aggcggacgc ggtcatccac agccccagga
    16441 ccagacccgc cagcaggcga cgccatcgtt cgagatcggt cggtttcatc gtcagcagtg
    16501 cgagcgccgg aagagagggt gctggcgttt ctgcaagttt tctgccactg gcatgggctt
    16561 tgcggacagc gtgagcgcca ctcgcatcca accccgacat cgcaggagaa atgacggtga
    16621 aattgccagg aacgggatta gcccttctca ctttcggtat cgcggtccgg gtggcttctg
    16681 gctcgcctgc tctggccagg gccgccttcg atcccttgcc tgccgtcggg aagtgagcgc
    16741 actgatgtgc tgtcgcatcc cgatcggcgc ggtcccgatg gtgctggccg tgctgctggc
    16801 cggttgcgcc caggcgccac ccgcgccgca ggtcaacccg gaaaggacgc tggtctttcc
    16861 ggctccgccc gatgcgccga ggttcgccta cgagcgcacg atccgctcca gtggcgacat
    16921 cgttccggac gaggcggctg gcgaactgcg ccgcctggtc acgggcgagg atgcgcgtgg
    16981 cgagcccttg tccaagccct acgcagtcgc ggtccaccga ggtcgcatct tcgtctcgga
    17041 cacggtgtcg cgcttcgttc ggctgttcga cgtgccggcg ggaaagcagt accgcatcgg
    17101 cgatgacgac ggtgccggtc agctgatcaa gcccatcggg ctcgacatcg atgcggcggg
    17161 caatctctac gtggccgaca tcggagccaa ggccatcatg gtctacgggc cggaccgtca
    17221 gttccttcgg cgcatcggtg gcgagaagtg gttttcccgg ctttcaagcg tcaccgtcga
    17281 tcccgagggc aagcgcgtct atggcgtgga cctcggcggt gtcagctccg agcagcatca
    17341 ggtccgggtg ttcgacggtc agaccggcca gcatctgatg gacatcggca agcgaggtgg
    17401 cggggccggt gagttcaatc ttccgagaga cctggccatc ggcgcggacg ggcgcctcta
    17461 cgtcgtcgat ggcggaaatt tccgtgtggt cgtgttcgac cacgacggcc gctatctgca
    17521 gagcttcggc agtgtcggca agcagtacgg tcagttcgcc cgaccgaagg agatcgccgc
    17581 cgatcgcgag ggcaatgtct acgtggtcga tgcggctttc ggcaacttcc aggtattcaa
    17641 ttccgacggc gagttgctga tgttcgtggg cgaccgttcg gagcgcgacg ggccggcgaa
    17701 gtacatgctt ccctccggca tcgcggtcga cgaggacggg cgcatctatg tcgtcgacca
    17761 gtggttccgc aagatcgacg tctaccggcc tgtggggctg accacctcgt ccggtttcct
    17821 ggctcgccgc gcggctccgc cgccgcgctg aaccttcccg ttcccgggaa gccggcttcc
    17881 tgaaagcggg gagatttccg gctccgtgca acgcaaagcc agcagccgtg cggctttcag
    17941 ggatatcggc cgaatggcac agaacctgca gaaatacggt atcgggtggg ggctttctgg
    18001 ttggcctggc ctgatgactt gatcaatcca acatcgagag gacgacatga acttgtcacg
    18061 gcacccgggc atccggaggt ggggttcggc gaccgcagga atgcttgcgg cgctgctcct
    18121 cgctctgtca ggaagcgcgt cggcgggaat atccgacacc aagcacaacc tcgggtccgg
    18181 tgcgggcccg gcaggccgca atcaggtcag cgacaccgcc gagatctgcg tgttctgtca
    18241 cacgcctcac ggaggcagca ccgatgcgcc ggttccactg tggaacaagc gtctgggtgc
    18301 agtcggtgca gcagccggag gcggcagcta cacgacctac gacacgctgc agaccccatc
    18361 gctggatggc accgtggctg ctgtgggctc gatctcgatg gcgtgcctgt cctgccatga
    18421 cggtacccag gcgatggaca acgtcatcaa tgcgccgggc tccggcgggg tgctggccga
    18481 cggtggcggt gtcgatggtc gcaactacac ctggtccggc agcacggtca gtgcggcagg
    18541 tcgcatgaat ggcggtgcgg cgctgatcgg aacggatctc tcgaacgatc acccgattgg
    18601 catccagtat tgcggcgggg gcctcgctgg cgcgggcacg acggtgtccg gcacctgcaa
    18661 ggacggtgac ttcgtggcgc cgcagacgca gaccatcaac acgaaccagg tcttctgggt
    18721 cgacaccggt ggtcccggca agcaacgcac cgacatgccg ctgtatcagc gcgcagcggg
    18781 tggcctcggg ccgatggtcg aatgcgcaag ctgccatgac ccgcacgtct ccagcgcaca
    18841 ggcgggcccg gccggtacgg ggcagtcttc tggcgaaacg ttcctgcgca tctccaacgc
    18901 ctccagcgca gtctgcacgt cctgccacgt gaagtaaggg cgacagcaca acggtccgat
    18961 atcgcggccc ggccctgacc ggtcgggccc ggtatcgcct cgccccgatg agtccaggaa
    19021 agatactgat ggctgcacgt atgcgtgttg cgaccggcgc agtgctgtgg ctgctttgcg
    19081 cggtcgtcgg tgccgcggaa accgtggtgg ctcctgctgc cgctgcgtcg acgcccgcca
    19141 ccgcgccatt cgccgtcgtc ggcgccgacg tgatcagcgt ggccgacttc cagcgcgcgc
    19201 tcggtgtggc gatgcgcaag aagtattacc acgccaagcc tcccgaggcc gagttcgcga
    19261 agttccagcg ggaggtcggc gacgacgtcg tcaaccgcgt gctgctgctc aacgaggcgc
    19321 gtcgcagggg catccagccc gatcgcgctc gcatcgatgc cgcggtcaag ggatacgacg
    19381 cgcaatacaa ggacagcgcc aactggaagg tcaaccgcga caagatgctt gccgcggtgg
    19441 tcccgcagct cgagcgagag agcctcctcg agcgactcga ggtcctcgtc aaggcgatgc
    19501 ccgagccggc tccggccgtt gtccggaggt actacgacga acacaaggaa ctcttcatcg
    19561 agccggagca ggtcaagctc agcgtgatcc tgctcaaggt ggatccgagt tctccgcagg
    19621 cgctctggaa cagcgcgcgc gacgaaggtc aggccgtcca caagcggctg cttgcggggg
    19681 cggacttcgg tgaactggcc cggctgcact ctggcgaccg ctcggcgccg cagggtggcg
    19741 agatggatta cacgcaccgc ggcatgcttc ccgaggcgat cgacaaggtc gtcgagaagc
    19801 tgcggccgca tgaggtgtcg gagccggtac aggtgctcga aggcgtggcg atcctgcggc
    19861 tcgacgaccg tcgggtggcg cgccagcgac cgttcgatca ggtgcgcgac cgtgcggccg
    19921 acctctggca gcgtgccgaa ggcgagtcac gctggaaggc gctgatcgcc gaactgcgcg
    19981 gtgctgcgct tatccgcatc gacgaaagcc actatgcgcc gttgtgccgt gcgagcgaca
    20041 agccgtgcgc cagctgagcg gcggctccac gccggccgcc gcgcgagaag gtttcgcatg
    20101 cacagcgggc cgcgtgcggt agcttccccg cggcgcagat cctcccggcg ctggtggaca
    20161 gtgacgctgg cgatgcttct tccgggcacc gccgcacatg gcgtggccta ccttcccgga
    20221 tcggccgagc ccgatccgcc caccggcgat gcccccgcag ccgtgcccca ggcggccgaa
    20281 cccctgcgcg ccggaagcgg catcgagtgg aagctggctc cgtggcgcat gagcggcagc
    20341 gtggcgctgg acgggcgctg gctgcgactc gataacggca atcgcagcaa gcagggcgtg
    20401 ctggtcaccg atctcgaatt caccagctat gtctggaagc cctggttcat ccagttgcgt
    20461 gccggcctcg gtgcgatggc ctcggtcgaa cattccagcg cagacagcgg gtcgcgttcg
    20521 ggttcgggca gtggcagttc ggcgctgaac ggtcgcttca cgctgtcggc ctttccggcg
    20581 agccggtttc ccttcgaatt gcgcgccgat gccagcgaca gccagaccga tggcaattcc
    20641 ctggggggag cctatcggac gcatcacgtg gccgtcacgc agtcgtaccg tcccgagacc
    20701 ggcaacgaca actaccagct gaacatcgat tacagcaagg tgatgcccca ggagggcggt
    20761 gccgacacgc tgtgggcgtt ccaggcgacc gccttgcgac agtacgtcga gaacaccttc
    20821 gagctgggtg ccaacctcag cctgaaccgg cgcagcgaca ccggtgacag cagcgacatc
    20881 cgtaccctga cggcccgaca cagcttccac ccgacgaccg atttcctgat cgagacgctg
    20941 gcgacccaca acgacgtcca gttgcgatcc agcgttcccg ggggcgatct cgatttcggg
    21001 tccgacatgc ggcaggtctc cagtttcctg acatggcgtc ctcgcgaggg ggagtggttt
    21061 cacgcacccg atgcgccgct gtcggtgacg ggcaccgcgc gctggatcga ctccggcgcg
    21121 aacaatggtg gtggaggaca gcgattcagt gcgttcagcg ggacggtcgg tgtgaccaag
    21181 gaactgacgc gggcatggcg tgtgaccggc gcggtgtcga cgacccgcct cgatggcggc
    21241 agcaaccgcg ccgtcacttc ggcgaccgga aacgcctcgg tcacgtacgc gccgcaaggc
    21301 accgagctgg gtcgctggcg ctatgcaccg acggtgtcga gcaacctgag tgtcacgcgt
    21361 tcctccgact cgcaggaccg tcgggtgatc ggcctgcagg cctcgcacag cgtctcgcgc
    21421 gactttctcg tggcgggaag cgagagcctg tcgctgacgc tgtccgagag cgcggcggcg
    21481 ctgcgtgaat cgccgtctgc cgattcggcc tacgccatct cgcactcggc gggcgtgttc
    21541 tggcaggttc agggcgatgg ttcgagccag agtttcgcga gcgtctcggt cagcgactcg
    21601 ctgacctggg cgcacggccc gggtcacttc tccctcggca atctgcaact gagccgacgc
    21661 acccagctca cgcgctacac gagctggtct gccaacctca cgctgcaggc cacgcgcaat
    21721 cagtccacgg agatcgaccc gttcacgggc gaacaccgca agcgcggcgg gggctggcag
    21781 cagttccaca gcggctcgat caactacgag agccagcggg tgttcagtgt gccccgcctg
    21841 cgcttcacgg cggtgctgtc ggccaacagc cagcagatcg agcgccgctc ggcgggcgac
    21901 atcgacgcgc cgcgcgagcg catcaccgag tcgctcgagg gacgactcga ctatgcgatc
    21961 gggcgcctgg acacccggct gagcgctcgt gtcgctcgca tcgacgaacg cacggtggcc
    22021 gcggtctatg cgcgagtgca gcgccgcttc tgaatttcct cactgacacc ccttaccgga
    22081 gcaagccttg aaattcctac gactcctgct ggccaccgtg gccctggcgc ttgcgctggg
    22141 atcgacggtg cacgcggaat acggcgatgt cgtcatcaac aactactccg atgccgcagg
    22201 catgcggccc gtggtgtttc ctcactggtt ccaccgggtc cgcttccgct gcaaggtctg
    22261 ccacgccgac ctggggttcc agttcaaggc ggggggcaac gagatcaaca tgctgaagat
    22321 catcgacggt cagttctgcg gtgcgtgcca caacggcgag attgcctggt cggtcgagaa
    22381 ctgcaacctg tgccattcgg gcaagccgaa caccggcacc catgtgcacg agagcacgct
    22441 gcagaagctg gcagcgcccg cacacgcggc atcggcggcg gcccaggccc ccgcgcgcaa
    22501 gtgaggagta ccgacgtgcg aaaactcctc atcagctttc tcatgaccgc agcgattccg
    22561 gcgttcatgg cggatgcgct tgcacaggat gcaaaggtca gccccaagtc gatctacgac
    22621 tccacctgct cggcctgcca cggccccggc atcctcggcg cgccgaagtt cggagacgcg
    22681 gcgcaatggg cgccgagggc gaaggccggt atcgatgccc tggtcaagag cgcagtcgcg
    22741 ggaacgacca agggcatgcc gccgaagggc gggcgcatgg acctgagcga cgcccagctg
    22801 cgcggtgtca tcgagtacat gatggccggt ggcgcaccgg ctgcctcgac ggccaaggcg
    22861 cctgcggcat cgacggctcc ggccgcgaag gccgctgtgg tcgcgtcacc gccggcgccg
    22921 gccacgaagc cggcagcccc ggcggcggtc gcgcaacccg tcgccactgc agccccgggt
    22981 gcggtggcgg cagctccgaa tccaccgctc accccggagg tcaacgcgtt caaccggttg
    23041 ctcaagcccc tcgggaaatt caatcgaccg gccgttgaat ccggaatcca cgatccggcg
    23101 aacgacatga cgctgcaact gcagccaccg gcggctgcat tcgcgcagtt gccgaagagc
    23161 atggcgggca atcacgtcga ctgggtcaag gccctggatg gcaaggccat cgctccgcgc
    23221 tgggatcgca acgatccggc tgctgcggcc atcgtcatgg acctgaacat cgtgcgcgag
    23281 gtcaagggat cgatgccgga cgtcgtgtat ccgcacaagc agcacaccga atggctcgat
    23341 tgctcgaact gccatccggc gatcttcatt ccccagaagg gcgccaacca gatcagcatg
    23401 gcgtcgatcc tgctgggcca gaagtgcggc gtgtgccacg gcaaggtcgc cttcccggtc
    23461 tccgagtgcc gcctgtgcca ttcgcgaaag aaggccgtcg cagccagcgc cggcgaggcc
    23521 aagccgtgag gaacgacgag ccgcaggcct cgggcggctt gcgtgcgctc gccggcgtgg
    23581 ggttgctcgt ggcggccgga atggcgtcgg cggccgaaga tgcgcctccg tcgcagatcg
    23641 accagcggat ccggctcacg gcccagttga tcggggattc accggcagcg cagcgcatcc
    23701 tggccagcgg caaccagcag gctgtctcga atctcgaagc gggccgtgcg cacctggcac
    23761 aggcccagga cctgctcgcg aagggcgacc agtccggagc gctgcgccac gtcgacgagg
    23821 cgctgcgaca cgtcgccgtc gctcgtcgcc tcgtgcccga cgcgccctcg cgtcaggccg
    23881 cggcaaggca gcgtttcgag gttcagtcca ggcacctgga atccatgctc ggcgcctggc
    23941 gcgtgcgcct tgcgccggga gatgccgacg atcgcgaact ggtcgaggcc gacggactgg
    24001 tgcgtacggc gctgggcctg gctcatgacg gccgccatga agaggccaac caggttcttc
    24061 agaaggccga gcgtcagatt ctttcgggaa tgagccgcct tctgaaccag cggacactgg
    24121 actacacggc gcggccgacg actctggcag aggcctttca ggtcgagctc gcacggcatc
    24181 gcgaactggc ggacctggtt cccatcgccc tgcacgacct gaagccggat gccgaagcca
    24241 ccaccctgat cgctcgctac caggaaacca gccagaccct tcgcaaccag gctcttcagc
    24301 gattcgaagg gggccagacc gaagacgcgc tggcccacct gcgaaacgcg ctgctctatc
    24361 tgcaacgcgc actcaacgcg gccggcgtca cgacgcctga acccactgga tccatccaat
    24421 gaagaacatc cttgccggcc tttcgctggt tttcggactg acactgatct ccgtcgacgc
    24481 acatgcgcag gacagcgtgc agctggagcg gcgagtccag tcgatcggca ccttgatcga
    24541 gtcatcttcc gcggcgcgtc agatcgagtc gagcggcgtt gccgaggcac gcgagaagcg
    24601 caacaacgct cgcctgatcc atcgcgaggc cgcttcgaca ctgggctccg gggatctggc
    24661 gaccgcggcc cgcctgctgg atcaggctgc gcgcgaaatg atggagggtg cccgcctggc
    24721 acgacccgat caggttaccg gagaaaagac gaagcgcgac ttcgatgcgc gcatggagag
    24781 cgctcgcgcg ctgctggccg cgcagcagcg gatcacgaag gagaagtcgg cgggtcgcga
    24841 ggaccaggag gccacgcgca gcatcgaatc gcagatcgcc ggcgcgcagg cactcgcgtc
    24901 gcagaaccgt tacgacgaag cgcgccccgt gctggaccgt gcctacctga cggcgcgggt
    24961 atcgatcgca tcgatgcggc gcggcgacac gctggtccgg tcgctgacct tcgcttcccc
    25021 ccgagaggag tacgactacg agatcgaccg caacgacacg caccggatgc tgctcaccgt
    25081 gctgctttcc gatcgcaagg acgcggccgg cgcgatgcca ccgaacatgc agcccttcgt
    25141 cgacaaggcg gcggtgctgc gcacggaagc ggaagcgcag gcccgccggg gcgatcatgg
    25201 gggggcgatc aaggtgctcg aggattccac tcgcgaactg gtccgtgcca tccgcactgg
    25261 cggcatctac atccccggtt gatcgtggac gatgaagccg ctgcgaagaa gaccgggccg
    25321 ctggctcgcc gcagccctgg tgcttctgac cgcggcggct ctggccgcga catcgctgtg
    25381 gacccggctc gagaaggacg gagtgcacga ccccctcggc ccggccatcg gcgtgctgca
    25441 gcaaccgggc gaggcgctcg agtcgctgcc ccgggattcg gctggaaacc aggtgaactg
    25501 ggtgaaggcc atcgacagcg gtgccatcaa cccgcgcacc aacatccttc ccgagaccaa
    25561 ggttcgcgtg cccgacaagg acatgatcat cgccaggtac ggctcgatgc cggccgtgaa
    25621 gtttccgcat cgccagcaca cgctgtggct ggactgcgcg aactgccacg accacctgtt
    25681 caaggccgag gcgggcgcca atcgattcag catgatggcc atcctcaacg gggaacaatg
    25741 cggcctgtgc catggcgccg tggcattccc tctgaccgag tgcaaccgct gccacagcgt
    25801 gtccaatgcg tcgctgccac cccggaccgc gcgatgaagc ggctgcgctg gatcggtctg
    25861 gccgcctgcc tctggcttca gccgcaggcg catgccgagt acggcgacgt ggtgctgaac
    25921 aagcgatccg accagcaggg tatgcggccc gtggtcttcc cgcactggtt ccatcgcatc
    25981 cggttccgct gcaaggtgtg ccatcaggaa ctcggattca agatgcgtgc gggtgccaac
    26041 gacgttcaga tgtccgacat catcgacggc aagttctgcg gcatgtgcca caacgatcgg
    26101 atcgcctggg gaccggaccg ctgcgacctg tgtcattcgg ctctgccgcg cacacccgcc
    26161 ggcatcgtcg gcggtgcgca gacttccggg ccggggcgct ggtaggccat gcaccggcgc
    26221 catttcctgt cgatcggtct gctgggcggc tgcgctgcag cggccgaaga ccccgtggca
    26281 ccgcgggagg cccgactgct gtcgccctgg cgcacggtcg atggcggatt cctggcgcag
    26341 ccctcgctgc tcgcgggcct gccgccgagg cctggcgcgg gcatgtatgt ccggctgatg
    26401 gcctctggcg cgctggcgct gcgcggcaac gacctgctcg tcgccgacat gggaacggcg
    26461 cggctctggc gcgcggacgt catgttgaac acgctgaccg cgatcgcagg cgctccggtc
    26521 tttcccaaca cggccgtggc gctgggtccg gacctctcgg cctgggtgct cgacacggct
    26581 tcgcgccagg tgctgcgctt cgggcgcgat ggccgcttgc tgcagaccct gcgcgccggc
    26641 agcgcgaccg cgatggcgct cgccgacagt ggcaacaccg ttctcgtggc cgagagcgcg
    26701 ctgtctcaat ggtcggagtt gcgcgccgtg ggttcgcgcg ccgtgccggt tcgtcccgag
    26761 atcgagggca gcgagcgggt gatcggcgtc gatggtctgg ccctggcccg caacggcctg
    26821 ttcgtgctcg atcgtgccga ggcggttgtc catcgtgtcg gtcgtgaagg gcgcgtcctc
    26881 gcgacgctgg gtgccggcga actggcccag ccgtcggcca tcgccgcaga caggtccgat
    26941 cgcgtcttcg tcgtcgacga gatgcagcgc gtgctcaggt tgctccagga cggccagcct
    27001 gcggtgacga tcgacgcgga ggaactcggc gtgcagcgca tcggcggcgt ggcggtcgat
    27061 gagcgcctgc tcgccgtgtc ggattcgctg acgggccgcg tggtgatcca tcagctggcc
    27121 gcggcggcca cctcccgatg agggcgctgc tcgcgttgat cctcctcgtt ctcgcgggtt
    27181 gtgccagcga ccccggggcg gcctcgatcc ggggtgagct gcgctacggc atggacgacg
    27241 cgcccgaggg ccgcaagctg atgtggccgg caccgccgga gatcccccgc tttctctaca
    27301 cgggcacgct gaccggtgag cacaacttcc gtcgcccggc agccgagacc ggcgggttga
    27361 aggccttcgg acgctggatc ctgggcctcg acgacaggcc gccggctccc gtcgtgctgc
    27421 agcgtccggg tgcggtggtg agcgacgatc atggccgggt ctacgtcagc gacacgagcc
    27481 ggcaggccgt cttcgtcttc gacgaggccg cgggcgagtt gctggtgtgg gaccgtgcgc
    27541 agggactcgt cggattcgtc gcgccctcgg ggctcgcggt cggcccggga cgcctctacg
    27601 tggccgatgc cgatctcgct gcggtattcg tcctcgacgc ccgtggcgag ccgctgtcgg
    27661 tgatcggtcg cggtctgctc aagcgcccca ccggactcgc gcgcgacccc gagcacgggc
    27721 tgctgttcgt ggcggacacc tatgcgcatg acatcaaggt cttcacggac gacggggtgc
    27781 tggtgcgcgt catcggcggg cgtggcgatc gcgatggcga gttcaacttt cccacccacc
    27841 tggcgttcgc gcagcacgag ctctacgtca ccgacacgct gaacagccgc gtgcaggtct
    27901 tcaacgcgga aggcgagctg ctgctgcgaa agttcggaac gcgcggactc tatctgggca
    27961 acctggtgcg gcccaagggc gtcggcgtcg acagcgaggg aaacgtctat gtcatcgagt
    28021 cctactacga cagcatgctg gtctactcca atcgcagcga gttcctgctg tccatcggtg
    28081 gcaccggcac ggccaccggt cgcttctacc tgccggcggg ggtgtgggtc gactcgaaga
    28141 atcgtgtgtt catcgcggac atgttcaacg gtcgcgtcgt gatgttccag tttctcgggg
    28201 gcagctgatg cgcggcctga tccgcgtgct ttcgctgatg gccgtgctga gcggcttgat
    28261 gccggcgtcg gtggccgatg cgcagcgcgt ggccgacgtg cgcggcacgc gccacaacct
    28321 gtctgtcgac ggcctcggat cgacccatgc ggcgcccggc ggtaccagcg aggtctgtgt
    28381 cttctgccac acgccgcatg ccgccacgca ggcagacctc ggcggcacgc cattgagggc
    28441 acccctgtgg aatcggcgcg tgcccgcggg ctccacgtac acgccgtaca cgtcgagcac
    28501 gatggatgcg cagtcgatca ccgacgggct caatgcgcag cccggcggca gttccaagct
    28561 gtgcctgtcg tgccatgacg gcacgctggc catcggcaac gtgaacgtgc tcaacggcaa
    28621 ccagaacgtc acgatcccga tgacgggtac cggccccggg ggctcgatgc cggtgggcga
    28681 gggcaccgcg agcggcttca cgcgcttcct cggcacggac ctccgcaacg accacccgat
    28741 ctcggtgacg ttcaacagcg cactcgccac gcgtgatggc gaattgcgcc tggtggacgc
    28801 acagcagcgc tggccggcgg gaagcgggtc ggtgctcggc ctgcgctcgc cgggcttcaa
    28861 gccgttgctg ccgctggagc cgacaggcag caccggagcc ggacaggtgc agtgcgcgac
    28921 ctgccacgat ccgcacctgc gcgaactgga cgagacgaag ggcaaccaga aattcctgag
    28981 ggcgcagcgc ttccaggaac tgactccgtc ggcaacgcac aacgcctcga gcgacatcat
    29041 ctgcctgtcg tgccacgaca agaacagcgg cttcggaatc tggtcctact cggcccacgc
    29101 gcggcccgat gtcgccgagg aaacctaccg cgatggcgcc gccacgctgc gcgaattccc
    29161 gaccggtctg ccggtctgga aggccagttg cctgaactgc cacgacaccc acaccgttca
    29221 gggtgcacgc cggctgaccc gtgaaggcac cgacgcgccg ttgcccgccg gcaatccgct
    29281 ggccgcgcgt cagggcggca atccggcgct cgagaacacc tgttatcagt gccacacgac
    29341 ggtggcgaaa tcggccttgg cgagcacggc gggtgtgccg aacatcgaga gcgagttcgc
    29401 gcgcgcgatc cgcatgccga tcacgacctc ggaccagggt ggcgccgccg aggaggtgca
    29461 cgacatctcc tcgaacttca gcgacatcac catcgactgc acgactccca acaatcgttg
    29521 cggggccgat ctgctcgaac ccaagtcgca gcttgcccag cgtcacgccg agtgcaccga
    29581 ctgccacaac ccgcatcggg tgatccgcaa ccgcctgttc aacgccaatc cggtcagccc
    29641 cgactcgaag ggaacgcacg ccatcgatgc cgtgacggga gtgacgccga ccaacctggc
    29701 cagcggcgtg ctgcgaggat cgtggggcgt cgagccggtg tacggatccc cgtcatttca
    29761 cgtcaacccc ggcaacttca cggtgaagcg cggggatccg ggagccaacc catcgacggc
    29821 cgtgacgcag ccgtacatga cccgcgagta ccagatctgc ctgaaatgcc actcgaacca
    29881 cgcgtacgac aacatcccgc cttcgctcgg acgcagcggc gggacgtcgc ccggcaccaa
    29941 tggcctgacc acgtacacga accaggcgcg cgaattccag ccgccgctgg cgcaccaggg
    30001 cggcggcatc ggcgacccca agagcctggg cagcaatggc ggagcaggct cggcgtacac
    30061 catcgggaac caccgctcat ggcacccggt catggggcag accgggcgcg acatcgacag
    30121 gcgcggcatc agcgcggcca agaatccctg ggcgctgccg tttcgcaacg cagtcggcac
    30181 gcagaccatg tactgctcgg actgccacgg ttcgaaggcg accgcgtccg gctcgctgca
    30241 gcccgacagc ggcgtctccg gaccccacgg atcggagaac gacttcctgc tgaaggctcc
    30301 cttcaacgcc agcgacccct cgctgtgctt caagtgccac gaacgcgagg tctattcccc
    30361 caccggcgac tcccgcaagt cgggcttcta caccggcttc tgctgccgcg gcggccgcga
    30421 cgacggcaag gatcagctcc attggtacca ctggaacaag atcctcagcc acgatcccag
    30481 ccggacactc aagtgcaact ggtgccatgc ggcagtgcct catgggtgga agaacaaggc
    30541 attgctggtc aacctgaatg acgtcggcga ggaggctggc tttcctggcg gaaaccggga
    30601 gttccggatg gattcgccct cgcaggccta caaccaggag ccctactacc tgaacgccaa
    30661 gctcaaggtg cgcaccttcg cgaccagcgg caactggact gacaccaact gtggctcgaa
    30721 caactccgcc acgaccttcg gcaccaacgg caacagcacc cagagcggcc gggactggat
    30781 gaaggacgtg tgcagcaatc caccatgaga cacggccgca tcgttgctca cttcggttcg
    30841 ccgcgcacca cgctcgcggg tctggcgctg ctggccgtgg tggtgctggc gggttcgtcg
    30901 tccggcggca tggtctggcc gctgacggca gtcttggcgc tgctgtccct gaacctttgt
    30961 gccgccctcg tggtgcatcc ggccttcagg cgccagttgc cgctgctgac cgcgcacctg
    31021 gcgttgctgg cgctggtggt gctggcggcc gtcggacgcc tctgcgcgct cgacggccga
    31081 ttcgagctca cgcagggcgt gcccttcgac ggtcgcctga tagaccagtc gcgcggtccg
    31141 ctgcaccagg agcgcctgca gcggctgtcc ttccggcacg atggtttcga gatcgactac
    31201 gcggcgggcc gcaagcgagg agccacccgc aactcagtca cctgggtgga tgccgatggg
    31261 gccgctcgca gcgatgtgat cggcgatcac cggccgctgg tgctcgaggg gcaccgcatc
    31321 tacaccagcc cgaacaaggg gttcgctcct gtgatgcgct gggagccccg gcagggcgag
    31381 ccggtggtcg gtgccgtgca tctgcccagc tatccgatgc acgagctgcg ccagtcgcgc
    31441 gagtggacgc tccccgacgg tcgcgcggtc tgggtgatgc ttcagttcga cgaggtgctg
    31501 atcgatcccc aggctgcggc ggcattccgg ttgccgggcg agcatcggct ggtcgtgcgc
    31561 gtcggtgagg tgcgggccga gctcgccgcg ggtgatcgac tcgatctcga tggtggaacg
    31621 ctggtctatg acggcctgcg aacctggatg ggttaccgcg tctcctacga cccgacgctt
    31681 gcgtggttgc tggcggcctc gctgctggcg gcgttgtcgc tcgcactcca ctacgcgctg
    31741 aagttcttcg ggcaggctgc aacagccccc cgcagtctga ctggggtcat cgatggctga
    31801 aggtctggcg ttgcactcgg cactcgcgct ctacgccgca gccttcggcc tggcctggat
    31861 gagcctgcgg accgagagcg gcctgcaatc ggggtacacc gcggcggcct cgttgttcgg
    31921 tggtgcgctg ctgctgcaca cgctgtccct ggcactgcgc tggcaggcgc atggccacgg
    31981 gccgttcacc acgatgcacg agatcctgag cagcaatctc tggagcctgg cgctggtgtt
    32041 tgcgctggcg gggtggggcg tgcgcgaggt gcgcgcgacg ctttgggccg tgtcgccggt
    32101 gctgctggtg atggcgatct ggatgttgct ggccgacgcg cggccgggtc acatgccgcc
    32161 gacctatgcc acgcctctgc tctacctgca cacgctggtg ggcaagctgt ttctcgggct
    32221 gctgctggtg gccgtcgcgc tgggtgccgt gccctggctg aggcgcactc cgtggggcgc
    32281 aaggcgcctg gcccgatggc ccgatgaccg ccgattcgac gaactggccc atcgcttctc
    32341 tgcctttgct ttcgtgtgcg agacgttgat gctcatcgtg ggcgccgtct gggcacagga
    32401 tgcctggggc cgctactggg cctgggatcc gctcgagtcc tgggccttcg tgacctgggt
    32461 ggccttggcg gccaccctgc atgcccgcat cgcgcttcgc ccgacgccgg cagctcacgg
    32521 cctgtggctg gcgctggtgt tcgccatcgc cttcctcacg ttctttggcg tgcccttcat
    32581 ttccacggca ccgcacaagg gggccgtgtg acccgcgccc gcaccgaccg ccgccgggcg
    32641 ggcgctctgc tgacgctgtt cgcggtgttc gccggcggct tcgcgttcca gtggcccggg
    32701 ctcgaggcga cccggcagtc cactgaatct gcagccagca gccggccgct ggcgagcgcc
    32761 actgcgcagc ccgagcagcg cagtgcggag gtgcatcgtc gcttcgatga cgccgtcgcg
    32821 atgctgcagg cccgcgacta caacggcgcc gtgacgggct tccagcgcgt tctcgaactg
    32881 gcgcccgacc tgccggaggc gcatgtcgac atgggctacg cactgcttgg gctgggacgg
    32941 gcgaaggaag ccctcgattc tttcgacggc gccacggcgc tgcgtcccga ccaggccaat
    33001 gcctactacg ggatggcgct ggcctacgag gcgagcgggg atctggccac agccaccgga
    33061 gccatgcgca gctacctgca tctggctcgc aacgagagcg aggcccacct tcgacgtgcg
    33121 cgagccgcac tgtgggaatg ggaagctcga cgcacgccgc gttgatcggg tcgcgcgcag
    33181 cggcttggcc gtgaacttgc atttccagtg gcatgccgaa cctgttccgc cctgaagcgc
    33241 tgcaattgca gcgccgtgtt ctcgtattcg ggaaatcatt cctgaacgcg gggttcccca
    33301 ggaggtcggc gtgaccgtct tctggatcgc cgccgcgctg ttcctgctcg cagcgctgct
    33361 gttcctgctg ccagctcttc ttcggccaaa ggccgacggc ttcagcgcgg gcggtatcaa
    33421 cctggccatc catcgggatc agttgcgtga agccgaacag gacctgagca ccggactgct
    33481 gacgctcgac cggttccagc agtcacgagc cgagatccag cagcgcgtgc tcgaggacac
    33541 cgtcgcgctg gccgatcggg tgatgctcgc gagtcctgcg cgcaggaccg cgatcgcgct
    33601 ggccgtgctg ctgccactgg ccagcgtgct cacctatctg caactcggtg atccggaggc
    33661 tgccgcgccg tcgctggcgc cgatgcaggc tcccgccggt gcatcggctc gtcacagcgt
    33721 caatgccgag cagatccaga agatggtcac ggcactggcc gagaagcttc gtgccgatcc
    33781 gaacaatgcc gagggctggc tgatgctggg acgttcctac acggtgctcg ggcgctatca
    33841 ggacgctgcc accgcgtttc gacatgccag cgagcagttg cccgccgatg caggggtgct
    33901 ggccgactgg gccgatgtgc tgggcatggc gcagggcaag cggctggttg gagagcccgc
    33961 gcggctggtc cagcgggcgc tggacatcga tccgaagaac gtcaaggcac tggcgctggc
    34021 gggcagcgtg gccttcgagt cgcgcgacta cgcagccgcg cgcggctact gggaacgact
    34081 gatcgccgtc gttccacccg actcggagat ggcccgttcg gtgggcggca gcatccagga
    34141 agcgatgcag ctcgagggat ccggcggggc ggtggccgcg cccgccgtgg cctccgcccg
    34201 gcagacggtc agtggcagcg tggagatcag ccccgaactc gccgcccgtg tcgaggcggg
    34261 cgacacgctg ttcgtcttcg cccgcgccgc ccaggggccg cgcatgccgc tggccatcgt
    34321 caggcgcgca gcgacgctgc cctacgcatt cacgctggat gactcgatgg cgatggcacc
    34381 gaacatgaag ctgtcgggcc agtcgcaggt ggtgatcgga gcgcgcattt ccagaagcgg
    34441 caacgccacg ccgcaggcgg gagacctcac gggccagagc gcggtcgtca gcccgggcgc
    34501 ccagggcctg cgcatcgtca tcgatcaggt tcagccgtga tggggacctg ctgctgacag
    34561 gcgtgtcgta gttgacaccg ctcgcgtgtg gcagacgtca gaatcggcag atcgcggtct
    34621 cgctgaggta cgacaaatga acagatccgt tgcgatgcgg ctgccggcag cggtcttcgc
    34681 tgtgctgtgc tgcgcaggga cagcctgtgc ggcggccgag gacccaccca aggcagcgct
    34741 ggacgaggct cgcagcgctt ccaccgaatt gctgacgggt gtgcgcagcg aactgctcaa
    34801 ggcgatcgat tccagcggtc cgctgcgtgc catcgtggtg tgcaagtaca cggtgcccga
    34861 aatctcgtct gcggtttccc ggaagtacgg ggcccgcgtc acgcgcgtga gcctgcgtcc
    34921 cctcaacccg tcgctcggct ggggcgatgc ctgggagcag aaggtcctga tggatttcga
    34981 cgagcgtgtc gcgaagggcg aaaagcccga gggcatggag cacttcgaag tggtcaacga
    35041 gcccgcgggc aggttcctgc gatacgtgcg cgcgttgccg ctgctgccgg tctgcgcgag
    35101 ttgccacgga cccgccgacc agatctcgga accgatacgc agccagtttg cgcacgacta
    35161 tccccacggg tattcgacgg gaactcccgt cggccgcatc cggggcgcgg tcaccttcaa
    35221 gaagccgctg tgactacttc gtttcgccct tcagatagac ggcggcgtcg gcatggccca
    35281 gtgctgccgc gcgctcgatc cacttggtgg cctctgccgg gtcctgggtg acgccgctgc
    35341 ccgtcaggaa caggatgccc aggctgtact gggcatccgc gttgccctgg cgcgcggcct
    35401 cgaaatacca ttgcgcagcc aggcgattcg ggtcggtgcc ggcagccagc ggtgccagcc
    35461 cgtaggcata catgttgcct aagtggaact gtgcttcggc caggcccttg cgcgccgctc
    35521 gctcgaacca ttgcagagcc aatgcatcac tttcgcgaac accgtgaccg tggtaataca
    35581 acagacccag cctggtttga gcgatcggat cgccggcctt ggccaggggt tcgagcagtc
    35641 gaacggcggt ctgatagtcc ttccgattca cggccgccac ggcgtcgttc aacggatcgg
    35701 cgtatgaaca gacaatgatg gctgaaagac tgatgccaac aatggaacgc ttgagcagca
    35761 tgaccgatct cctctggatg agaagcggct tcaatgcaat cgctgtaccc gaaggccgct
    35821 tgggcgccga gggcaatccc ccatgaacac catcgttgcc gatccaggtt ccgagctgcc
    35881 gaaacgcaag tccctccggg ccaagggcct ggtggcgacg ctcgtcctgg tggcctacct
    35941 gctgggtgcc gtcgtctacg tgtcgatgga gagggccaag atctacgaga gcatgcaggc
    36001 gctcgaccag ctgtcgcagc acgagaaggc ggtcgccctg accgaatccg ccgtgaacgg
    36061 cgcgcgcatc gacgtcaaca tggccagcaa cgcggcgcat tcggaccccg gcatgccgga
    36121 ggagatttcc ctctacatgg agtcctgcga caagctgttc gcagcgctcg acgcgttcga
    36181 tccgtcctac acgctgctgc atcgagccat cacgcgcagc tacgaggccc tggaagctgc
    36241 acccgacagg tcgaactgga tcgacctgcg cgaatcgctg tctcgcgcaa ccgacgagct
    36301 ggccatccgg cgtgccaacc tgctcgagca gcgcgaggta ctcacgctcg gataccagcg
    36361 ccagtacgac gcggtgacga tcgaatcgct ggcgcttgcc gcgctcggcg tcgccgtgtt
    36421 cggatcgatc gcggcctggt tcttttcccg gcttgcacgc gacatcgggc gactcgagga
    36481 ccatgccggt cagatcgttc gcggaacgcg gggcgtggct ctcgatgtgg gacgcgagga
    36541 tgaactcggc cgcctgatgc atgccgtcaa tcgcatggcc gtggatctcg acgagcgcga
    36601 gaagcagatc gaagtcgatg cgcatcgtcg tttccatcag gacaagatgc tggccgtggg
    36661 cgcgctggct gccggcatct cgcacgaggt caacaatccc ctggcggtga tcaccggggc
    36721 ggcgcaggaa atcggcgcaa tggatggtgc cgagctgcct cagtccgtga ccgacaacgc
    36781 gcggttgatc ctcgcgcagg ccgagcgcgc cgcgcaggcg gcccggcgcc tcgccgaggt
    36841 ggcggctccg caggcttcgg agcgggactg ggtcgatgtc aacgcgatgg tccgtcgcgt
    36901 cgcgcagttg atcggctacg accgccgata ccgtcacttc gtgttcgaca ccgacctcga
    36961 tctgcaactg cccgccattc atgtctcggg caatgccgtg cagcaggtcc tgatgcagat
    37021 gctctccatc gcctgcgatg cgatggtggc cggtcacgcc gagtcgaaga cgctgagact
    37081 gcagacgctg ctcgtagatg gtcagatcga gatgcagttg ctcttccccc cggtgctcga
    37141 tttcgatcgc cccgaagtcc agcgcgtcct gttgctcagc cgagccatca tcgagcctct
    37201 cggcggaaag cttgcgtttg gtcaaacagc gggaccactg caacgcatta agctcacctt
    37261 ggttgccgaa actggcatcg aacaaggatg aattcaatgc ccgagctgaa tgtgctggtc
    37321 gtcgacgacg agccggccat ccggcaggtg ctgtcggctg ccatcggcaa ggcgggctac
    37381 agcgtcgatg cggcaggctc tgcggctgaa gcgcttgcca agctggaaaa ggcctctttc
    37441 gacgtcgtgt tgagcgatgt cttcatgccg gtgaccgatg gcatcgagtt gctgaagctg
    37501 gcgcgcgaac gcgggcacgc cgccaccttc atcatggtca cggcattcgc ctcggtggac
    37561 tcggcgatcg aggcgatcaa ggccggcgcc tgggactaca tcaccaagcc ggtacgcaac
    37621 gaggaaatcc tgcaccgcct cgagcagatc gacgcgctgc gcggcttgcg cgaggagaac
    37681 cgagccctgc gcagtctggt gatgggcagc ggcgaaatgg cgttcgcgtt cacctcgccg
    37741 gcgatgcagg ccaacgagcg gctcgtcagt cgcgtggcac ccaccgacag caccgtactg
    37801 atcaccggcg agagcggaac cggcaaggga gtcaccgcgc gtcgcgtgca cgagctctcc
    37861 tcgcgacgtg acggaccctt cgtctgcgtg aattgcgcgg ccatcccgga gaacctgatc
    37921 gagagcgaac tgttcgggca cacgaagggg gccttcacgg gagcggaccg ggtgcgcaag
    37981 ggcctgttcc tgcaggccga tcgcggcacg atatttctcg acgagatcgg tgaacttccg
    38041 ctgtcgcttc agaccaagct gctgcacgtc ctcgaggcca aggaggtgcg cgcgctgggt
    38101 tccgaggtca gtcgcaaggt cgatgtccgc atcgtgactg cgacgaatcg agacctcgcg
    38161 gcgatggtgg cggaagggcg attccgcgag gacctgtatt tccggctttc ggtgttccag
    38221 atacagatgc ccccgctgcg cgaacgtcgg tcggacatcc ccgggctgct gcggcacctg
    38281 atggcgcagc gcggggtgcc ttccggcagc agcgaggcgc tgacgcttga ccccgaggcc
    38341 gaggacctgc ttgtcaacta cagctggccg ggcaatgttc gccagctcca gaacgtgctc
    38401 caccgcgcgg ccatcctcgc tgatggagga tgcatccgag tcgccgatct gccggccgag
    38461 gtgtcgcatg tccttcccgc cgtcaacgag cctgcgcccg tcgagggtgg cgaatgcggg
    38521 ctgcgcgaaa gggtgaggcg cttcgagcac tcgctgatcc gccgcgccat cgatgaggcg
    38581 ggtggcgatc gccggcttgc cgcgcagagg ctcggtatcg gcttgtccag tctctatcgc
    38641 aagctcgagg agaccgcccc ggagtccgcc acgggctgag tcactggcat ggatttcgca
    38701 gtcctgtgcg tcatcagcgg atgggcctgg aggttgcgaa gtggtcggat cgaagttgcg
    38761 agcctggttc gcggtggcac tgtcgctggt ttcctgtctc tgccaggcca tcccgtccga
    38821 tgaccacgcg cgcggtgttc tggcctacca gcgcggggac gtcgtcgctg caatgtcggc
    38881 gctgcgagag cccgcgcggg cggggtacgc gccatcgcag gccttgctcg cgttcatcct
    38941 ggatcgatcc gacttcagtg aagaggcatt ccggctgtac cgggacgcgg cggcacagaa
    39001 cgatgccgaa ggccacgccg ggctggccag cctgtacttc gccgggcgag gcgttgccaa
    39061 agatgagaag ctcgcactcg agcatttctc aaaagcggct gatctcggtc acgcggcatc
    39121 catcgaaacg gttgccaatg cgttcctgaa gcggcagatg ggcctcgatg ccgacggccc
    39181 cgatggcgcg gccgcggtgg ctgcgctgac gcgcgctgcg gggcaggatc acgtggcttc
    39241 ggccgaggcg ctggcccgcg cctaccagag cggtcgctac gggctggctg cggaccctgc
    39301 ccaggcagcg aactggcagg ccagagcggt ggccatccgc aagaagcagg cggcgcccgt
    39361 ggccaaggtg tccaagtgaa cgcgcgatcg atctggcttc ccgtggcgct gtcgtggtct
    39421 gtcgttgccg gtgcggccga tgcgctgaag ggcgccgacc tgtatcgcag tcactgcagc
    39481 cagtgccatg gcaatgacgg gcgccccgtg ttgccgaccg cgcccgactt ttctcgtccg
    39541 acggcactgc tgaagtccga cctcacgctg ctcgccagca tccgcgcggg ccgcggcggc
    39601 atgccggcct accaggggca gctcagggac cgcgagatcc tcgacatcgt ttcccatctg
    39661 agaacccttc gatgaggtcg ctgctttggc gtggcctggc ggtcctggtg ctgtgcatgc
    39721 cggcgctggt gctggcacag aaggtcaggg acgcgttcga tgtcggacag tccgtctacg
    39781 tgcgggctct cacggtcgag cccgcgaccg gggcgctgtg ggtcggcacg tcggcgggcg
    39841 tgcacgaggt cgaccttggc agcggcaagc tgcgcaacac gttcacacgc aagcatggct
    39901 tggccaacga gtacgtcttc gccgtcggcc tcgacctgca gggcaacaag tggttcggca
    39961 ccaacgcggg tggtgtttcc cgctacaaga acggaaagtg gaagacgttc ttcccgatgc
    40021 acggcctcgc ggactactgg gtctattcgt tcgcccagca gcgcggcggg gacttctgga
    40081 tcggtacctg ggcgggggtc aaccgggtcg atgcgaagac gggcaagttc tccacctacg
    40141 tgaaggaact cgtcaacgag tgggtctacg gcatcgccgt cgacgcgcag gaccgggtct
    40201 ggttcggcac cgaaggcggg gtgtcgatgt acgacggcaa gcgctggcgt tcgtggacgc
    40261 acaaggacgg cctgggcgca ccgaacagtg acgatctgcc tttctccacc aacaccgggc
    40321 ttggaacccg atcacggcac gacctgtcgg tcagcgcgga agggccggcg acctacaacc
    40381 ccaactacgt cttctcgatc ctgtctgcag ccgacggcac cgtgtgggcg ggaacctggg
    40441 gcgccggcgt ctcgcgctgg gacgggcgcg cctggacgaa cctgaccacg aaggacggac
    40501 tggctggcaa catcgtctac agcgtcgcac aaggtgccga cggtgtgtac tggttcggca
    40561 ccaacaacgg cgtctcgcgc tacgacggca aggcgttcaa gaacatcggt gtggccgagg
    40621 gcctgcttga aagaaatgtc tacgcactgg ccgtcgcacc tgacggcgac gtatgggtcg
    40681 gcacccgtcg gggtgtggct cgcatctcgc gttgagatcg ggtgcaaaca gcaaccagag
    40741 gaagtctgat ggccatcaac gcgcggatcg tgggcattgc cctcatcgcc ctcggagcgg
    40801 cactggccgg ggcttaccag ctgggacgcg gcaacgcggc tgccaacgcg gcggctctgc
    40861 cggactctgc ctccacggtg gcggccccgg caccgacagc ggcctctccg gtccctggcg
    40921 atgcggtcca gccgcccggc catccgcacg atcccgccgg gctgcagcgt gaaggcaagg
    40981 tgaccggcgc ggacccggcg cagaaattca cccacttccg cgtcggcaac aagaacgtca
    41041 agcgcatcta cgtggacggt gacacggtgt gggtcggaac ctccggcggt gccgttcgct
    41101 acgacaccct gaccgacgac tacaagctgt tcgacatcaa gagcggcttg ctgtccaacg
    41161 gggtattcca cgtgggcaag atgggggagc gcgtggtcgt cggcacctat ggcggcggca
    41221 tgtccctgct cgatccgaag acgcagacct gggaaacatt caacatcccg gaggggttgg
    41281 gcgatgcctt cgtctacgac gtgctgacca cgaagaatgg cgatgtctgg gtggccacct
    41341 ggtctggtgt gaaccgcatt cgaggaggcg cgctgaagga tcggtcgaag tgggaactgc
    41401 acacggtcca gagcacgcag ggcggcctgc cgaatgactg ggtctacggt ctggcggaag
    41461 gcaggaacgg ggatgtctgg cttgccaccg agggggggct ggcccactac acggaaggtc
    41521 gctgggagaa ctggaaccat gaaaagggcc tgggcgcgtc ctacgaaatg gtcaagaacg
    41581 acatccagta caagaacgat ccgtccaagg tttcgcagca ccatgcaaag cagaaacagg
    41641 agatgggact ctccaacatc gatgtggcct acaacccgaa ctacatcgtt tcgctggcgg
    41701 tcgacgtcca gggcgtggtc tgggccggaa cctggggcgg cgggctttcg cgcttcgacg
    41761 gaaagacatg gaagcagttc acggtggcgg acgggctgcc cggaaatcac gttttcatgc
    41821 tgcacatcga tcccaagggg cagatgtggg tcggcacgaa caacggactg gcgaaaatga
    41881 aggatggaaa gttcgaagtg ctgaccacga aggacgggct gttcggcaac gcggtcttct
    41941 cgatggccac cacccgcgac ggcacgctgt ggatcgggag tttcggtggc gttgccagga
    42001 tcaagccttc ctgacgcggc aacagggatg tcatggaccg ttcgacgaat cactctctgc
    42061 tgcacacgct gaagccccac aactccggtg ccctgctcac gctgctgctg aagatcgccg
    42121 acgacgcggt gatcgttgcc gatgccgacc agcaggtggt cctcttcaac gaaggagccg
    42181 agcgcatctt cggctacccc gcacaggctg tactcggaca ggccatctcg atgctgctgc
    42241 ccgaggcggc gcgtgccgcg cacgtcgacc atctgcgcaa gttcgcgcta tcaccgcgtg
    42301 ccgcacgccg gatgggtgaa cggcgcgaca tcagtgcgag acgctccgac gggagcctct
    42361 tcgatgccga ggcgtcgatc tcgcacgtgg aactcgacgg cgaaacctac ttcaccgcga
    42421 tcctcaggga tgtgagtgaa gcacgagacg ccgagcgagc gctgatgcgc agcgaggcac
    42481 gcttccgcgg tctggctgcc gcagccccga ccggcatatt ccagacagac atcgacggca
    42541 actgctgcta cgtcaacgat cgctggtgcg agattgccgg catgacggcg gccgaatcgc
    42601 tcggtcgcgg ctggttgagg gcgctgcatc ccgccgaccg cgcgcgggtg cagctggagt
    42661 ggaccgccgc cgtgaacgag aagcgcgcgt tccatctgag ctaccgattc ctgcggccgg
    42721 atggaagcga ggtctgggtc gtcggcgacg cggtgggcag ccacgaatcc agcggcatgc
    42781 ccggtggaca catcggcacc gtgaccgacg tgaccgagag ccaccatcag gcgctggcgc
    42841 tcgagcgcgc gaagtcggaa gccgaagccg cagcgcgcgc caagagcctg ttcctggcca
    42901 acatgagcca cgagatccgc acgccgctga acgccgtggt cggcatgacc acgctgctgc
    42961 tcgatacggc gatgaccgac gaccagcgcg actttgcgcg aaccatccgc gccagcgggg
    43021 attcgctgct ggagatcatc aacgacatcc tcgactactc caaggcggac gtcggcaagc
    43081 tcgagatcga gcagcgtccg ttcgacctgc ggcgctgtgt cgaggagtcg ctcgatctgg
    43141 tgacgccgcg cgcgctcgag aaggacctga acctggccta cctgatcgag gaaggcacgc
    43201 ccgaggccct ggtgggcgac gccacccggg tgcgccagat cgtcgtgaac ctgctgtcca
    43261 acgcagtcaa gttcactcac cagggtgagg tgttcgtgtt cgtggacagc gagcgggtcg
    43321 atgaagagac gcaccgcatc catttcgccg tcagggacac cggcatcggc atttctgccg
    43381 aacacctgcc gcgtcttttc cagtcgttca cccagatcga tgcctcgacc acgcgcaagt
    43441 acggaggcac cggcctcggc ctggcgatca gccggcgact ggccgagctg atgggtggca
    43501 atgtgtatgc cgaaagcgaa ccggggcagg gctcggtctt ccacgccacg gtgctggtga
    43561 gggaagccgc gtttgcggaa ccagccgaat tcctgctgcg caatccgcca gccctcgtgg
    43621 gcaagcgcat cctcatcgtc gatgacaacc agaccaatcg ccgcatcctg acgaagatgg
    43681 tgctgctgtg ggggatggtt ccatcgacgc tgccttcggc gctggaggcc atggatcggg
    43741 tgcgccatgg cgaggccttc gacgtggccg tcatcgacat gagcatgccc gatgtcgacg
    43801 gtctggacct ggcatgcgag atccgcaagc ggcgcagcgc acaggaactc ccgatcgtca
    43861 tgctgacttc gctcggccag cggcaactgg ggcagaacgc cgagctggcg gcctgccact
    43921 gcaagccgat caaggccagc ctgcttttcc agacgcttgt cgaggtgatc gcaggcacgg
    43981 ctgctcccca ggacccgatc aggccgcagg cgcgtgccga gccgaccgtt ccactgcgca
    44041 tcctggtcgc cgaggatcac ccgatcaacc agcgagtcac ccagcggctg ctccggcatc
    44101 tcgggcacga atcggatctg gccgccgacg gtcgcaaggc catcgaggcc gtcgctcagc
    44161 gcgtctacga cctggtgctg atggacatac agatgcctga actggacggg ctcgaggctg
    44221 cgcgtgccat cgtcggagcg cgcggcacgg ccggcctgcc acgcatcgtc gcgatgacag
    44281 ccaacgccat gcccggcgat cgcgagtcct accttgccgc gggcatggac ggctaccttg
    44341 ccaagccgat cgagctgacc gacctcgcgg cggtcgtctc gcaagcggcc gtgcgcgcac
    44401 aggcccgaca ccacgacggc accttcgatt cggtgctgga ccacgccagg ctggaacatc
    44461 tgcgctcgat gcaggacgag aaccagccca gcctcgtgcg cgacctgatc gacatgttcc
    44521 tgaccgattc gtcgcatcag gtccagcaac tgaaccgagc ccacgccgct gccgacgccc
    44581 acctgatgcg tggactgtcg catcgccttc tttccgcgac gcagaacctg ggggcctccc
    44641 ggctgtcgcg cctgtgcatc gaactcgagc gcctggcgaa gctcggccag accgacgcgg
    44701 ctggtgcgtt gctggccgat ctggaacgcg agcgcagcct ggcgcacgag gcgctggtgg
    44761 ccgtgcgcct gcgctactga gtgcagcgct gcatgaccgc ttccggcagc atcggttgcc
    44821 acgcgcgggc tctgctgtcc gctgccctgg cgctgtgcct tctcgaagcc gatggtcctg
    44881 catggtgtgc accgtgtcac gcaggcttcg ccgcgatggg ctcagggtgc ccgggttgag
    44941 gccgatatgg agggatgcgt cgcgtcctgt cgattcttct cgtgcttgcc gcagcagctc
    45001 cgttcccggc cctggccgct gcgacggtct ggctgtgcgg tctttccgaa gacctggttc
    45061 gcctggtctg catcgcggac accggtccgc agtcgcccga agcggcgccc gaggcggctg
    45121 ccaccgtcaa cggagtccag tttccgctcg acccgcgccg cacctacgtg gtcgacctgt
    45181 ggagcccgcc gaccgaagtc gccttcgtcg agcagcttgc gcgcgccacc atctgctatc
    45241 gcagccccgg gtgcgaggta ctcctggtca gcccgttgcc ggcgcctgcc gcgtcggatg
    45301 ggcgacagag gcgcagccgc tagcgaactg ctgcaccgcg ccgcagcgcc cggtttagcg
    45361 tgattgatcc catgctgatg cgttcggccc ttcagggcgc accgcgaccc ggatttctgg
    45421 gaacgcccgc tgacccggcc acaggccgcc gcagcgccgt ggcggcctgc gcactgtggg
    45481 tgtcgcttaa tctgtgtccg ctcctcgcag caggccagcc cgctgccgcg ccgccgggcg
    45541 aagtccaggc agggcaggtg cttcccgaag cactgctgcc gggtctcaac ggaccatccc
    45601 gcaagctgtc cggctaccgg ggtcggccgc tgatcatcaa cgtctgggcc agctggtgcg
    45661 gcccgtgccg gcaggagatg gcgtcgctgg agaggctcgc ctggcagccg aacgatccgt
    45721 cgttcgcggt gatcggcatc tcgacagacg acgatccgga cagcgccaga cggctgctgc
    45781 aggcaaccaa tgcgaccctc agccatttca tcgacgacag gctgcgactg gagcacatgc
    45841 tgggcgcatc gcgcctgccg ctcaccgtgc tggtcgacgc gcaggggcgc gtggtcgcca
    45901 aggtctacgg cgctaagcaa tgggatggtc ccgaggcgcg gcgcttgatc gaccaggcgt
    45961 tcggaaatcg cccgagccca gccaggcggt agctgaccgg tgcaccgcca gtccggcgag
    46021 gtcaagcccg cggggggggg gcaggttcga ccgctggccg ccgggttcta atctcccccg
    46081 gagacaggtt cccggccatc gctggcgatg gactgttcaa cgggtcgacc ggctgggaaa
    46141 gtacctggcg actcacaaca agaaggaaaa gaaatgagag tgacgcaggg aagcaggaag
    46201 ctgctgatgg cggccgtcgt ggcggctggt tcgctgaacg cgcaggcggg cgcccggtac
    46261 gacttcggcg aagaccgcaa cgtgaccttc ggcatcggca cccgctacag ctacacccat
    46321 gcagagcgcc cggattccgc aggaggcggt catgtcaacg accccaacct ggacagcgca
    46381 cgcctgttct tcggcgcgtc tctgagcaag aacatcaagg ccacgttcaa caccgagtgg
    46441 gacggtgtcg agaaccgggt gcgcgtgctg gatatctacg cccagttcga gttcctgccc
    46501 gagatcaatg tctgggcagg gcgtctggtg tcgcccagcg accgtcacaa catggccggg
    46561 ccgtactact cgatgggtgg tggctactgg ccctgcgtcg cctcacgcta cggctgcaac
    46621 ggcggcatct tccgcgcccg tgacgacggc ttcgtggtct ggggcaacgt cgccgacagc
    46681 cggctgggct actcgatcgg cgccttcgac ggccgcacct tcggcatcgg cgcgctggac
    46741 gaatcgcagg cccgcggcgc cggcgtcaag tcgtccggct cgctgatgta cgccgggcgg
    46801 ctgcagtacg acttctggga gaaggagacc ggctacttca gcgcggccaa tcacctgggc
    46861 aagcaggaca tcctggcgat cggcgtggcc ggtcgctatc agaagaaggg tgtgctgacc
    46921 accgggggcc gtggcgacta ctggtcctac aacgtcgact tcctgttcga gaagagcatc
    46981 aagggctcgg gcggagtcgc ggtcgaagcc gcctactacg actacgacac cgacgacgtg
    47041 gttcgggccg aacaaggcaa ggcgtactcg gccggtgtgt cgtacatctt cgagcagccc
    47101 gtgggctggg gccagttgca acccttcacg cactggcaga aattcaaccc cgactcgaac
    47161 accgacaccc ggcagatcga cgtcggcgtc aattacgtga tcgacggctt caatgcgcag
    47221 ctgacggcga tgtacaccaa caccaaggtc acgagcagca agagcctgga caagttcgtg
    47281 ctggctttgc agttgcagta ctgagtcaca tccgggtcca tgcatccaat cactcgagat
    47341 cagcgctctt gccaacaccg aggcattcgg ggttttggat gccttcgcgc acagctgctg
    47401 ttcctgctgc cgagggcgtg gggtgactgg cgcagcgaag taaaggagga gggcctgtct
    47461 tttcaggccc gggggagcaa gcccggacac aaacagtcct gaggactgtt tgtgcctggc
    47521 gagcgcctgg ggctctgccc ccaggcatga gcggagccgg tcaccgcatg ccctcgacag
    47581 cgctgcgcga aagctcgcat gcggacggcc gtcgaacacg aaccgccggc tgatagatcc
    47641 ctcaaccgaa cacgatcgtc ctgtgcccgt tgatcaggat gcggtcctcg aggtggttgc
    47701 gcaggccgcg cgcgagcacg gccttttcca cgtccttgcc gcagcgcacc aggtcctcga
    47761 ccgcgtcgct gtggtcgatg cgcatcacgt cctgctcgat gatcgggccc tcgtccaggt
    47821 cctcggtcac gtagtggcag gtggcgccga tcagcttgac accgcggtcc caggcctggt
    47881 gatacggctt cgcgccggcg aagctcggca ggaagctgtg gtggatgttg atgatctgtc
    47941 ccgggtactt gcggcacagc gacggcggca ggatctgcat gtagcgcgcc agcaccatcg
    48001 tgtcggcgcc tgacgactcg aacagtgcct ccagtttcgc gaaggcctcg ggcttgttct
    48061 ccttcgtcac cgggatgtgg tggaacggca gcccgtgcca ctcgaccagg ccgcggaaca
    48121 cctcgtggtt ggacgcgact ccgacgatgt cgacgtcgag ctcctggctc ttccagcgcg
    48181 ccagcaggtc gtacaggcag tgctcctggg tcgacacgag catcagcacg cggcggcgca
    48241 ccgcgctgtc gctgatgcgc cagtccatgc cgaactcggt ggccacgacc tggaagcgcc
    48301 gcttcagctc ggcgtgatcg aagggcagcg agtcggcacg cacctcgacg cgcatgaaat
    48361 agcgcgccga gtcggcatcg gcgtgctggc tcgaactgag gatccagccg ccgtgctcgg
    48421 cgaagaagcc ggtcacgcgg gcaacgatgc cgacgcggtc ggggcaggaa aaggtcaggg
    48481 tgtagtggtg ggtgcggttc atggggcctc gcggggtcag ttctgggcag acacccgcga
    48541 ttgtcccgtt tcgccggcac ttggcttgca tacgggttgc ttcgaccgag ccgcgcagcc
    48601 tgccctgcgg cccccgttca tgacgacccg acccgagaac tgcccatgaa ccaccgtccg
    48661 atccgctcga tcctcatcgc caaccgctcg gagatcgcga tccgcgtgat gcgcgccgcc
    48721 tccgagatgc gaatccgcac cgtggcgatc tactcgcagc aggatcgcca ggcgctgcac
    48781 cgcttcaagg ctgacgagag ctacctggtg ggcgagggca agaagccgct ggcggcctac
    48841 ctggacggcg aggacatcct gcgcatcgcg cgcagcgccg gcgccgacgc catccacccg
    48901 ggctacggat tcctgtccga gaaccccgaa ttcgccgatg cggtgatcgc cgccggcatc
    48961 cgctggatcg gcccgtcgcc cgacgtgatg cgcacgctgg gcaacaaggt cgcggcgcgt
    49021 cacgcggcgg tggccgccgg cgtcgcggtg atgccggcca cgccgccgct gccgctggac
    49081 gtggccgaat gcgccaggct ggccgccggc atcggctacc cggtgatgct gaaggccagc
    49141 tggggcggtg gcgggcgggg catgcgggtg atcgagaacg agggcgaact ggctggctcg
    49201 ctggaagcgt cgcgccggga agcgctggcc gccttcggca acgacgaggt gtatttcgag
    49261 aagctgatcc gccgtgcccg ccacgtcgag gtgcagatcc tcggcgacca gcacggcaac
    49321 ctggtgcacc ttcacgagcg cgactgcacc gttcagaggc gcaaccagaa ggtggtcgag
    49381 cgcgctcctg caccgtatct ggacgccgcc gggcgcgccg cgctctgcga cagtgcgttg
    49441 cggttgatgc gctcggtcgg ctacacccac gccggcacgg tcgagttcct gatggatgcc
    49501 gacgacggca ggtgctactt catcgaggtc aacccgcgca tccaggtcga gcacacggtg
    49561 accgagatga tcaccggcgt cgacatcgtc aaggcgcaga tccgcatcac cgaggggggg
    49621 cacatcggtg tcactgaagg cgaccaagtc gggtcccctg agggctcgcc cgaagaagcg
    49681 agcggcctgc atcccaccgg cgtgccgccg caggacaaga tccctctgaa cggccacgcg
    49741 ctgcagtgcc gagtcaccac cgaggacccg gagaacggct tcctgcccga ctacgggcgg
    49801 ctcgccgcct accgcagcgc ggccggcttc ggcatccggc tcgacgccgg cacggcctac
    49861 ggcggcgcgg tgatcacgcc ctattacgac tcgctgctgg tcaaggtgac cgcctgggcg
    49921 ccgaccgcgc gcgagaccat ccagcgcatg gaccgcgcgc tgcgggagtt ccgcatacgc
    49981 ggcgtgtcga ccaacctgca gtttctcgag aacctgatca accacccgac gttcggcaac
    50041 gacgtcaacg gtgagctgac gacccgcttc atcgagaaca cgcccgaact gctggccttc
    50101 gccgcgcggc gcgaccgggc cagcaagctg ctgcgcttcc tgggcgaggt ggcggtcaac
    50161 ggcaaccccg aagtgcaggg ccgcacgttg cccgcgctgc cgctgcccaa gccggtgctg
    50221 ccgaaggtcg acctcgaatc gccgatcccc gacggcaccc gccagcggct gcaggccctc
    50281 gggccggccg gcttcgcgca gtggatgctg gcgcacaagc ccgcgctgat caccgacacg
    50341 acgatgcggg atgcacacca gtcgctgctg gccacccgca tgcgtaccgc cgacatgttg
    50401 ccgatcgcgc cgtattacgc ccgggagctg tccggcctgt tctcgctcga atgctggggc
    50461 ggcgccacct tcgacgtggc gctgcgtttc ctcaaggaag acccatggca gcggctcgcc
    50521 ctgctgcgcc aggccattcc caacgtgctg ttccagatgc tgctgcgcgg ctccaacgcg
    50581 gtcggctaca ccaactacgc cgacaacgtg gtgcgccact tcgtcaagca ggcggcgacg
    50641 aacggcgtcg acgtgttccg cgtgttcgac tcgctgaact gggtcgagaa catgcgcgtg
    50701 gcgatcgatg cggtgatcga gtacggcggc ctgtgcgagg gcgccatctg ctacaccggc
    50761 gacctctacg acagtgcgcg cggcaagtac gacctggcct attacctcgg catcgcgcgc
    50821 gaactgcaga aggccggcgt gcacctgctg gcgatcaagg acatggccgg catctgccga
    50881 ccgcgcgcgg tggccgcgct cgtcaaggcg ctgaaggccg agaccgggct gccggtgcac
    50941 ttccacaccc acgacaccag cggcgcgtcg gctgcgtcgg tgctggcggc catcgaggcc
    51001 ggctgcgatg cggtcgatgg cgcgctcgac gcgatgagcg ggctgacctc gcagcccaac
    51061 ctgtcgtcga tcgccgcggc actggccggc accgaacgtg acccgggcct gccgcaggac
    51121 gcgctgcacg ccgcgtcgat gtactgggag ggcgtgcggc gtttctactc gccgttcgag
    51181 tccgagatcc gctcgggcac ggccgacgtc taccgccacg agatgcccgg cggccagtac
    51241 accaacctgc gcgagcaggc acgctcgctc ggcatcgcgc accgctggac cgaggtgtcg
    51301 cagacctacg ccgatgtgaa ccggctgttc ggcgatatcg tcaaggtcac gcccacctcc
    51361 aaggtggtcg gcgacatggc gctgatgatg gtcgccagcg acctgacgcc tgcggacgtg
    51421 atggacccgg cgcgcgaggt cgccttcccc gaatcggtgg tgtcgctgtt caagggcgag
    51481 ctgggctttc cgcccgacgg tttcccggct gctctgagcc gcaaggtgct gaagctgggc
    51541 gaaggcgatc agccacccgt gccttaccgc cccggcgatc ggctgtcgcc ggtcgatctg
    51601 gaggccgtgc gcgcccgggc cgaagccgag tgcgaactgc cgatggacga cagccagctg
    51661 gcctcgtacc tgatgtaccc gaaggtgatg cgcgactact gcgagcacac ccggctcaac
    51721 ggtgacacct cgatgctgcc gacgccggtg ttcttctacg gtgcgcagcc gcagcaggag
    51781 ctggccatcg agatcgatcc cggcaagacg ctgctggtgg cgctgcaatc gatctcgccc
    51841 gatggcgatg tcgcgcagaa ggtgcagttc gagctgaacg ggcagtcgcg caccgtgcgc
    51901 gtgctgcgac ccgacgccgg cgatgccgcc aagggtcggc cggtcgccga ccccaccaac
    51961 ccgctccatg tggccgcgcc gatgcctggc gcgatcgtca gcgtcgccgt caagccgggc
    52021 cagcgagtct cggccggcag cacgctgctg gcactcgaag cgatgaagat ggaaacccac
    52081 gtcgctgccg agcgcgacgc cgaggtcgag gcggtgctgg tcgtgccggg cgaccgggtg
    52141 caggcgaagg acctgctgat cctgttgaag gcggtgagct gatcggtcgc ggtgcggtgg
    52201 atcagatcct ggtgcgccga gtccagaggg tgtcggccaa ggcgatgagc agcatggatg
    52261 cgccgaccag cggaaagacg attccaccga ccgccagcat cgccagcaca tgccacgact
    52321 tgcgggtgtc ggctggctga ggcggcacgc ccaggcttcc cgacggacgg cgcttccacc
    52381 acatcacggt cgctgacacg cacagcaaca cgatggcagc gcacgcgaag accatcagcc
    52441 atcggttgac ctctccgtac tgttgcccca gatgcacgtt gatactccac tccagcgacc
    52501 ggccgagcgg cccgtagtcg gcgtagctca tgtcgatcag cggtttgccc gtgtactgat
    52561 cgagatggat cacacgttgg cgcgtcagat cgctcggata gacagaggcg ctgtatgcgc
    52621 cggtggctcc ggccgggggg gtgacggcat accccgggga aatgcccagc gcttcgaagc
    52681 gggtgatcgc ctcgttgagg ctgatcgggg tgtgcgcact caatgccgat tgcggcaccc
    52741 gggcctgctg cagcgaccag gccgcaggtg ccgaatgcgc caggtgttcg tcggacatcg
    52801 gcacggcgac gcgcacgcct gcgggatagc cgaagttgtg accgttggcc cactcgttca
    52861 cctttgaacc ccacagcacc gaccagggca tgccggtgac cgccaggaac acgatgaacg
    52921 cggcggcgat gctgccggtg atcgcatgca ggtcgcgcca gaacaccctt tgctgcaccg
    52981 atccgcgcac ggtgctcacg ccaccggcct gtccccgggg ccaccacagg tacaggccgg
    53041 tggccaccag caggatgcac caccctccgg caatctcgac gaggccgctc gccaccggcc
    53101 cgaagtactt caggctgtgc agcttgcgca ccgtccacat cagcgtgccg cgatcgggca
    53161 ggaccccagt cactcgagcg cgataggggt cgacgtagac ggcgatcttc tcgcctgtcg
    53221 gcgccagtat gtccacctcc gccgctgcag aggctgtcgc cggaggcacg tacctgaaca
    53281 cctgtccacc ctgcgacgcg actgcggtgt cgacagatgc ctgatgggtc ctcggtgatg
    53341 cgcccgccga ttccacgcgc tggatgtcgc gatgccacca ggcatcgatc tcgtggtgga
    53401 acaggtagag cgcaccggta cctgccagc
//
LOCUS       DAOORV010000010        53354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_697_length_53354_cov_8.880242, whole genome shotgun sequence.
ACCESSION   DAOORV010000010 DAOORV010000000
VERSION     DAOORV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 53354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_53354_cov_8.880242"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1376
                     /locus_tag="PKV56_02715"
     CDS             117..1376
                     /locus_tag="PKV56_02715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOX66751.1"
                     /translation="MHPNALLELATELLHRVMQFDSPADNVVSDFFRQNRGLGMRERH
                     TLAETTYTVLRQRLLFQHLAQSGKGEMERRLVLLGWQGNAAFLRAALQEHEVLWLEQV
                     GAVDRTTLPEKLRHNLPEWLADRLQAEVGDEFWPLVEALNGSAPLDLRVNTFKAKRET
                     VQAEFAAANIEALPTPYSPWGLRIEGKPALHKLEVFLRGDVEVQDEGSQLLALMTDAK
                     RGEMVVDFCAGAGGKTLALGAAMRNTGRLYAFDTSGHRLASLKPRLARSGLSNVHPVQ
                     IAHERDDRIKRLAGKIDRVLVDSPCSGLGTLRRNPDLKWRQSPQSVEELKVKQAAILA
                     SASRLVKPGGRLVYATCSLLQGENEEIAEAFSSERVRDFKPLAAHDVLSSAHVAQADT
                     LVRGPYLRLWPHRHGTDGFFAAVWQRA"
     gene            1504..2727
                     /locus_tag="PKV56_02720"
     CDS             1504..2727
                     /locus_tag="PKV56_02720"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOX66752.1"
                     /translation="MNLLMTVSDGLLNWLANGLVQTTWWQIVLYALVATHITIAAVTI
                     FLHRSQAHRALDLHAIPSHFFRLWLWLTTGQVTKEWVAVHRKHHAKCETPDDPHSPQT
                     RGIKTVLLTGAELYRTETKNQETLVKYGHGTPDDWLERNLYTRYSWQGVGLMLLINLA
                     LFGAAGLTVWAVQMIWIPVTAAGIINGLGHWWGYRNFEAPDASTNISPWGIIIGGEEL
                     HNNHHTYPTSAKLSVKPYEFDIGWAYIRTLEMLGLAKVRKTAPQLKLGEIKPVADSKT
                     LEAIIANRYEVMAKYAAHLREECRSELARLKAEGGENSARWKDMHLAKRWLHRDSDRI
                     PLVVQERVATVCAQNDVLSKLVSMREELRQLWTRTNVSADQLVVDLQAWCKKAEDSGI
                     IALQEFALKLRAAHA"
     gene            complement(2821..2988)
                     /gene="rpmG"
                     /locus_tag="PKV56_02725"
     CDS             complement(2821..2988)
                     /gene="rpmG"
                     /locus_tag="PKV56_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOX66753.1"
                     /translation="MAKGGREKIKLESSAGTGHFYTTDKNKKTKPEKIEIMKFDPKAR
                     KHVMYKETKLR"
     gene            complement(3003..3236)
                     /gene="rpmB"
                     /locus_tag="PKV56_02730"
     CDS             complement(3003..3236)
                     /gene="rpmB"
                     /locus_tag="PKV56_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOX66754.1"
                     /translation="MARVCQVTGKRPMSGNNVSHANNKTKRRFLPNLQYRRFWLETEN
                     RWVRLRISNAALRLIDKVGIEKVVADLRARGEI"
     gene            complement(3421..4371)
                     /gene="trxB"
                     /locus_tag="PKV56_02735"
     CDS             complement(3421..4371)
                     /gene="trxB"
                     /locus_tag="PKV56_02735"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOX66755.1"
                     /translation="MSITSTHARVLILGSGPAGYSAAIYAARANLNPVLITGIAQGGQ
                     LMTTTDVDNWPADVDGVMGPDLMQRFQKHAERFNTQIVFDHINQVDFSDRPFKLTGDS
                     GSYTCDALIIATGASAKYLGLPSEQAFMGRGVSGCATCDGFFYRGQDVCVVGGGNTAV
                     EEALYLSNIANTVNLIHRRDKFKAEAILIDKLMDKVAAGKVKLHLWSTLDEVLGDASG
                     VTGVRTRNTKDNSTTELALQGCFIAIGHQPNTEIFAGHLEMKDGYIVTRGGLNGMATM
                     TSVPGVFAAGDVQDHVYRQAITSAGTGCMAALDSQRFLEQ"
     gene            4532..5194
                     /locus_tag="PKV56_02740"
     CDS             4532..5194
                     /locus_tag="PKV56_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX66756.1"
                     /translation="MAMLSNLDLIRRVPIFSLLTNDQAQGIADSVVKRRYRRGEIIVE
                     HGRKSNALFILLTGRARVLTADSRGREVILAVLQSGDYVGEMSLIDNEPHSATVRAEI
                     QTDMLILGRPEFARCLPENSSLSYAIMRGLVARLRSADRQIESLALLDVYGRVARSLL
                     DMAEVIGDAKIIRNKVSRQDLAKVVGASREMVSRVMKDLEERGMIETQENGSVVLKER
                     LT"
     gene            5376..7652
                     /locus_tag="PKV56_02745"
     CDS             5376..7652
                     /locus_tag="PKV56_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOX66757.1"
                     /translation="MASTWPRQARLLLGGVVWLLMLIALVTHSPQDAAFSTSGAGELA
                     LNKAGAIGAWLSDAAFFVFGFSVWWLMAVALHRWLGALAGLLRGADGEAAHLSPMRRA
                     WPLWAFWLGLALLMSASTALEWTRMYQWEPRLPGSHAGGVLGYLLGPLSMKWLGFAGS
                     GVLWIAAMVVGLSLSFRFSWLQVADAIGGRLDAWRDRRVERIEMAEDFRLGEQALRER
                     EDVVEVEHALHEDHAPIVIETPVVDVPKSERVVKERQKPLFIELADTKLPQVDLLDAA
                     PGRTETVSPETLEMTSRLIEKKLKDFGVEVRVVAASPGPVITRYEVEPATGVKGAQVV
                     NLAKDLARSLSLVSIRVVETIPGKTTMALELPNAKRQMIRLSEILGSQVYNDAASQLT
                     MGLGKDIVGNAMVADLAKMPHCLVAGTTGSGKSVGINAMILSLLYKAEARDVRLILID
                     PKMLEMSVYEGIPHLLCPVVTDMKQAANALNWCVGEMERRYKLMSKLGVRNLAGYNKK
                     IDDAAERGETMGNPFSLTPEAPEPLERLPHVVIVIDELADLMMVVGKKIEELIARLAQ
                     KARAAGIHLILATQRPSVDVITGLIKANIPTRLSFQVSSKIDSRTILDQQGAEALLGM
                     GDMLYMPSGTGLPIRVHGAFVSDDEVHRVVAYLKSQGEPNYIEGILEGGLLDGDSGEI
                     AVNDGGGPGNESDAMYDQAVAIVLQHKRASISLVQRHLRIGYNRAARLLEQMEKSGLV
                     SSMGTNGNRDIIVPARTE"
     gene            7649..8281
                     /gene="lolA"
                     /locus_tag="PKV56_02750"
     CDS             7649..8281
                     /gene="lolA"
                     /locus_tag="PKV56_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOX66758.1"
                     /translation="MKRDCVVRAAAWLACALFTAAPLAARADAVEALKSFVQDVKTGR
                     ATFTQTVTSPDGGKTKTSSGSFEFARPDRFRFAYTKPFEQLIVADGQKVWIHDVDLNQ
                     VSSRKFSQALGATPAALLAGGDLARDFDLLPLPAKDGLEWAKATPKARDGAFESMSIG
                     FRGKQLAAVEIVDNFAQRSRLTFSQFTANAEIAPQAFKFTVPKGADVLEQ"
     gene            8318..9670
                     /locus_tag="PKV56_02755"
     CDS             8318..9670
                     /locus_tag="PKV56_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOX66759.1"
                     /translation="MTSLPLPLEDTVPAPAPAASTADTTQPLAERLRPHTLAEVIGQK
                     HLLGPGKPLRSAFDSGRLHSMLLWGPPGVGKTTLARLMTDAFDAQFIAISAVLGGVKD
                     IREAVERAQAVQPSGRRTVVFVDEVHRFNKAQQDAFLPHVESGLFTFIGATTENPSFE
                     VNSALLSRATVHVLKSLDAADLVEMLARARRLLSSPAMSDAARDRLVAYADGDARRLL
                     NAYENLVEMTSEQPGLIEIDEALLERSLGEQLRRYDKGGEQFYDTISALHKAVRGSDP
                     DASLYWFVRMLDGGVDPRYAARRLVRMASEDIGLADPRALRMTLDAAETYERLGSPEG
                     ELALAQAVVYLAVAPKSNAVYRAYKSVRAFIAQDGTRAVPLRLRNAPTRLMKDLEYGR
                     GYRYAHDEEGGFAAGERYWPDDVDGPRFYEPVPRGLEIRIGEKLDELRRLNDAATKKT
                     "
     gene            9713..10636
                     /locus_tag="PKV56_02760"
     CDS             9713..10636
                     /locus_tag="PKV56_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX66760.1"
                     /translation="MDTFIQQIINGLVLGSVYALVALGYTMVYGIINLINFAHGEVLM
                     VGALTSWTVVSALADSGLPGWLMLLISLIAAMAVCAALNFGIEKIAYRPLRSAPRLAP
                     LITAMGVSLLLQTLAMIIWKPNPKPYPILLPSEPFNIGGAVINTTQILILVTTAVTLA
                     GLMILVNRTKLGRAMRATAENPRVAALMGVKPDMVISATFVIGAALAALAGVMYAANY
                     GSVQHSMGFLPGLKAFTAAVFGGIGNLAGAMLGGVLLGLIEALGAGYIGALTNNVLGS
                     QYQDIFAFIVLILVLTLRPQGLLGERVADRA"
     gene            10667..11788
                     /locus_tag="PKV56_02765"
     CDS             10667..11788
                     /locus_tag="PKV56_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66761.1"
                     /translation="MSSSPPRGKQFGLMLVAVIALLILPLIAQQFGNAWVRIVDTALL
                     YVLLALGLNIVVGYAGLLDLGYVAFYAVGAYMFALLASPHLTETFPAIAAMFPNGLHM
                     PIWVVLPLGAALAGLAGVLLGSPTLKLRGDYLAIVTLGFGEIIRVFLANLEQPLNITN
                     GPRGIGQIDSLSLFGWDLGKKWELFGFEVPSVTNYYYLFLALVILAIVICHRLERSRI
                     GRAWMAIREDEIAARAMGINTRNLKLLAFGMGATFGGVAGVLFATFQGFVSPESFSLQ
                     ESVMIVAMVVLGGLGHIPGVVVGALLLAALPEVLRYVAGPLQVMTDGRLDASILRQLL
                     IALAMIGIMLARPRGLWPAPEHGHGARAESGERGGVPHG"
     gene            11781..12557
                     /locus_tag="PKV56_02770"
     CDS             11781..12557
                     /locus_tag="PKV56_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66762.1"
                     /translation="MAEAVLKVAGVSKRFGGLQALSDVGITIERGMIYGLIGPNGAGK
                     TTFFNVITGLYAPDSGSFELAGQPYRPSAVHEVAKAGIARTFQNIRLFSDMTALENVM
                     VGRHVRSNSGLIGAVFRTAAFKAEEQAIAQRSQELLDYVGIGKYADFKARTLSYGDQR
                     RLEIARALATDPKLIALDEPAAGMNATEKLVLRELIDRIRRDNRTILLIEHDVKLVMG
                     LCDRVTVLDYGKQIAEGTPAEVQRDGKVIEAYLGAAHAQN"
     gene            12580..13293
                     /locus_tag="PKV56_02775"
     CDS             12580..13293
                     /locus_tag="PKV56_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66763.1"
                     /translation="MARTLLAVRGLKVAYGGIQAVKGVSFDVQEGELVSLIGANGAGK
                     TTTLKAVTGLQPMTSGDIEFMGQSIQGRGPWELVRQGLVMVPEGRGTFARMTITENLQ
                     MGAFTRDDKDIAADIDRVFAIFPRLRERSSQLAGTMSGGEQQMLAMGRALMARPKVLL
                     LDEPSMGLSPMMVDKIFEVINDIHRQGVTVLLVEQNASRALQLASRGYVMESGEITMN
                     GDAKSLLNDPKVRAAYLGE"
     gene            complement(13294..14358)
                     /locus_tag="PKV56_02780"
     CDS             complement(13294..14358)
                     /locus_tag="PKV56_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOX66764.1"
                     /translation="MSITVTLQPSGRSFPVQRDEPILTAALRAGIGLPYGCKDGACGS
                     CKCRLIEGRVIHGAHQLSALSVAEEENGYALTCCATPQTDVTLEARTVAGLGEMPVRR
                     MPARVSRIAKPSADVAIITLQLPANEALRYHAGQFVDVLLRDGARRSYSMANAPHTQG
                     DKPWIELHIRHLPGGLFSDQVFGAMKEKDILRLEGPFGSFFLREESPKPIVLLASGTG
                     YAPIKALIEQLIFKRSDRPVVLYWGCRTRADLYQHEAALELARELPQLRYVPVLSEAL
                     PSDHWTGRTGFVHEAVMADLPDLSGHQVYACGTPAMVDAARRDFVERCGLPQDEFHAD
                     AFLSAADKTTGTAARPEGAA"
     gene            14424..15311
                     /locus_tag="PKV56_02785"
     CDS             14424..15311
                     /locus_tag="PKV56_02785"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX66765.1"
                     /translation="MPALPSAFKLPVLLIVGCGDVGMRVLRLLEGRYRLLALTSSAAR
                     VESLRAAGAVPLQGNLDDPATLARLAGLADAVLHLAPPPLHGRTDPRTRNLLAALARK
                     GRVRCIVYGSTSGVYGDCGGARFNETRAVNPATDRARRRVDAETRLRWYGRSHGARVA
                     ILRIPGIYAKDREGGHPRERLLRGTAVLRAEDDVYTNHIHADDLARACVAALTRAWPQ
                     RAIHASDDTDLKMGDYFDLAAELCGLERPPRITRDEARERLSPMLLSFMSESRRLDNR
                     RLKRELRLKLRYPTVSDGI"
     gene            complement(15335..15928)
                     /locus_tag="PKV56_02790"
     CDS             complement(15335..15928)
                     /locus_tag="PKV56_02790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX66766.1"
                     /translation="MPPMPPITQALLLINIAAFCIDGFFGPWFTRLLALWPVGHGFLP
                     WQVVTYAFLHGGLGHLFFNMLGLWMFGSELERVWGPKRFIHFYTASVLAAAAAQLLIT
                     ALLGSTFPTVGASGGLFGLLLAFGMMFPNRTIVPLFPPIPMKAKVFVALYGGLELLLG
                     VTGSASGVAHFAHLGGMLGGFLMLRYWRGQSPFGRGR"
     gene            16066..16150
                     /locus_tag="PKV56_02795"
     tRNA            16066..16150
                     /locus_tag="PKV56_02795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16100..16102,aa:Leu,seq:caa)
     gene            16231..17148
                     /gene="cysM"
                     /locus_tag="PKV56_02800"
     CDS             16231..17148
                     /gene="cysM"
                     /locus_tag="PKV56_02800"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HOX66767.1"
                     /translation="MAKKQPIYRSLEDLIGNTPLVRLVRLPGADAERRGNVILGKLEG
                     NNPAGSVKDRPAISMIRRAEERGDIKPGDTLIEATSGNTGIALAMAAAIRGYRMLLIM
                     PEDLSIERAQTMRAFGAELMLTPRSGGMEHARDLADQLQRDGKGVVLDQFANGDNPRV
                     HYETTGPEIWRDTGGQITHFVSAMGTTGTITGTSRYLKEKNPAVRIIGAQPSEGSRIP
                     GIRKWPEAYLPKIYDPTHIDETASVSQADAEEMARRLAREEGIFAGISAAGACWVALE
                     LAKTVENATIVFIVCDRGDRYLSTGVFPV"
     gene            17188..17415
                     /locus_tag="PKV56_02805"
     CDS             17188..17415
                     /locus_tag="PKV56_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOX66768.1"
                     /translation="MLIQKELDTRGLNCPLPILKAKKALADMASGEVLKVVSTDPGSM
                     RDFQAFAKQTGNELVDQSSSETEFVHLLRRR"
     gene            complement(17426..18292)
                     /gene="galU"
                     /locus_tag="PKV56_02810"
     CDS             complement(17426..18292)
                     /gene="galU"
                     /locus_tag="PKV56_02810"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOX66769.1"
                     /translation="MKINKAIFPVAGLGTRFLPATKAQPKEMLPVVDKPLIQYAVEEA
                     YAAGIRQMIFVNGRNKRAIPDHFDTAYELEAELEAAGKHELLDLVHSIKPDDMECIYI
                     RQPKALGLGHAVLCAQCVVVNEPFAVLLADDLMVGKPPVLKQMVDRFADWQASIIAVQ
                     EVPDDQTRRYGMVAGTPVNDRMMDMTHIVEKPAPEDAPSRLGVAGRYILTPGIFKEIA
                     GLPRGVGGEIQLTDGIAALMRREKVFAFRYDGRRYDCGSKEGFLEANVELALTHPKLG
                     PGFREYLKKLEL"
     gene            complement(18417..21263)
                     /locus_tag="PKV56_02815"
     CDS             complement(18417..21263)
                     /locus_tag="PKV56_02815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOX66770.1"
                     /translation="MTELAKSFEPAAIEAHWGPLWEQSGVYEPTLDAAQPSFSIQLPP
                     PNVTGTLHMGHAFNQTIMDSLTRYHRMRGHNTLWVPGTDHAGIATQIVVERQLQEQKQ
                     SRHDLGRPNFVAKVWEWKQQSGSTITQQMRRMGDSVSWKHEYFTMDDKLSKVVTATFV
                     QLFEEGLIYRGKRLVNWDPVLKSAVSDLEVESEEEDGSLWHIRYPLEDGSGDVVVATT
                     RPETMLGDVAVMVHPEDERYSALIGKRVTLPLCGRTIPVIADDYVDRAFGTGVVKVTP
                     AHDVNDYAVGQRHGLPIIGVLTLDAVINDNAPEAYRGLDRFVARKRVVADLDAQGFLV
                     EVKKHKLMVPRCARTGQVIEPMLTDQWFVAMTKAGPDGRSIAQKAIDAVESGAVKFVP
                     EQWVNTYDQWMNNITDWCISRQLWWGHQIPAWYGADGELFVAKNEAEARAKAAAAGYG
                     GELTRDPDVLDTWYSSALIPFSSLGWPEKTKELDLFLPSSVLVTGYDIIFFWVARMIM
                     MTTHFTGKVPFHHVYIHGLVRDAQGKKMSKSEGNVLDPVDLIDGISLAPLLDKRTVGL
                     RKPETAPRVRKETEKEFPEGIPAFGADALRFTFASLASLGRSINFDSKRCEGYRNFCN
                     KLWNATRFVLMNCEGQDCGLAEHTKTQCEPGGSADKYLSFSQADRWITSELQRVEAAV
                     EQGFADYRLDNVANSIYSFVWDEYCDWYLEIAKVQIQTGDESAQRATRRTLIRVLETV
                     LRLLHPITPFITAELWERVAVVAGRKAAGSADSLVTARYPQPQLERIDATADAWIAQL
                     KGMVGACRRLRSEMSLSPDKRVPLMALGDGAFVLAATPLLKVLAKVSEVQIHTDEAAY
                     AQATLNAPVVEFGGTRLALHVEIDVAAETERLRKEITRLEGEITKGTAKLSNESFVAR
                     APAAVVAQEQARMAEFTATRDRLQAQVHRLSTTA"
     gene            21355..23889
                     /locus_tag="PKV56_02820"
     CDS             21355..23889
                     /locus_tag="PKV56_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX66771.1"
                     /translation="MSSPPESPAAVAPHEDPSIPLFEEDEPPGAAGRATGEIDAEPGL
                     AVPSSDNLLRGLNPEQLAAVTLPARPSLILAGAGSGKTRVLTTRIAWLIQTGQLSPGG
                     VLAVTFTNKAAKEMLTRLSAMLPVPVRGMWVGTFHGLCNRFLRAHWKLAGLPQGFQIL
                     DSADQLSAVKRVIKAMKLDEERFVPKTTTWFIAGQKEEGLRPKDVEVRDEQSRVLVQV
                     YQAYEEQCQREGVVDFAELMLRTYELMRDNQSLREHYQRRFRHILVDEFQDTNRLQYA
                     WLKMFAGPPGPNGTAVLAVGDDDQSIYAFRGAQVGNMADFEREFRVQQVIKLERNYRS
                     YGHILDAANELISRNSRRLGKNLRTEAGPGEQVRVFEATSDFAEAQWFIEEAQQLHRD
                     GVARRDIALLYRSNAQSRVLESALFNAGIPYKVYGGLRFFERAEVKHALSYLRLIENP
                     NDDTSFLRVVNFPTRGIGARTIELLQDAARASGRSLYQSVGAVAGKGGGNVQAFVAKV
                     DAMREATRGLTLREIIEHMLKASGLVDFYRTDKEGQDRLENLDELVNAAEAFVTQEGF
                     GKDAVALPVDEFSTPVPMGADSELTKVLSGLGIEAGAAAAQSVGGIASLITTPDAETG
                     EIMSPLAAFLTHASLEAGDNQAQAGQDAIQLMTVHAAKGLEFDAVFITGLEEGLFPHE
                     NSVSDLDGLEEERRLMYVAITRARKRLYLSCSQTRMLHGQTRYNIKSRFFDELPEASL
                     KWITPRNQAFGSGFTRDYQAAWARGSGLGSIVGAGRVATAPPSVIPKAPSHGLRSGQS
                     VFHTKFGEGVIVTLEGSGDDARAQVNFGRHGMKWLALSVAKLTPIP"
     gene            complement(24251..24946)
                     /locus_tag="PKV56_02825"
     CDS             complement(24251..24946)
                     /locus_tag="PKV56_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HOX66772.1"
                     /translation="MANIHFIGGEKGGVGKSLVSRLVAQYLIDKELPFLGFDTDRSHG
                     ALLRFYAGFASPVLIDRYEALDSIIEAAVEQPDRRILVDLAAQTHDPLVKWMDESGVL
                     DMAAEAGFTITYWHVMDSGRDSVDLLKKLLDRFGARLNYVLVRNQMRGDDFAMLEQSG
                     EQDRALGFGARVVSIKHLSDAVIQKIDASSSSFWAAQHDADKSRTGLGMMDRQRLKMW
                     LRDAYQQIDAASV"
     gene            complement(25152..27017)
                     /locus_tag="PKV56_02830"
     CDS             complement(25152..27017)
                     /locus_tag="PKV56_02830"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOX66773.1"
                     /translation="MNRNATERQHTNLPADMKHQRLKAWVDEIATLTDASGVYWCDGT
                     QAEYDRLCNQLVAAGTMKKLNPELRPRSFLATSDPSDVARVEDRTYICSQRKEDAGPT
                     NNWMQPSEMRTLLQSGDKALFRGAMKGRTMYVVPFSMGPLGSHISHIGVELTDSPYVA
                     VSMRTMTRMGRPVIDLLGSDGDFVPCVHTVGAPLEAGQKDVAWPCNSTKYIVHYPETR
                     EIWSYGSGYGGNALLGKKCFALRIASTMGRDQGWLAEHMLILGVTNPAGKKYHVAAAF
                     PSACGKTNFSMMVPPDGYVGWKVTTIGDDIAWIKPGTDGRLYAINPEAGYFGVAPGTN
                     HKTNPNCMDSLKSDVVFTNVALTDDGDVWWEGMTDTPPAHLIDWQGKDWTPAIAKETG
                     AKAAHPNARFTVAAGNNPVLDPEWDNAAGVAIDAFIFGGRRSTTVPLVTQARTWVEGV
                     YMAATMGSETTAAAAGQQGVVRRDPFAMLPFAGYNMADHFQHWLNLGEKLAATGAKLP
                     QIFCVNWFRKGADGKFVWPGYGENMRALEWMLGRVDGTAGGEDNVFGVSPRYDDLHWD
                     GLNFTREQFASVVGIDKAAWKQELVLHAELFKQLEFHLPSQLPATMARIEERLGT"
     gene            complement(27247..28830)
                     /locus_tag="PKV56_02835"
     CDS             complement(27247..28830)
                     /locus_tag="PKV56_02835"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOX66774.1"
                     /translation="MNCTRRRFLNRTGIAAAAGASAPAWLLGGCAPALRLETPGSAPA
                     DVARFDLGVASGHPSSIGMVLWTRLTGPGLPDRVDVRWELAHDEAFQRIAARGIETAE
                     AAWAHSVHAEPRGLEPGRWYWYRFQALGDRSAIGRTRTAPSTDARSGAAAPLRFAIAS
                     CQRYDAGHYAAWRDIAAQNLDLVLFLGDYIYESGSRPDGLRRHEGGVTRTLEEYRARY
                     ATYKRDPLLQAAHASAPWLTIWDDHEVENDYAGLQGAHLDTDFASRRAAAYQACWEHL
                     PFPSSARPRLDATGAAMRITGRLDWGALARIHLLDDRQHRAPQACPRPGQGGSNTVAL
                     KDCPALRDPQRSLLGLDQERWLATGWDLERSWNLVGQQTLMARHSWSDPASGGTYWTD
                     GWDGYVASRNRLLGTVAERQVPGVVVLGGDVHAHVVADLKADFDRPEGPTVATEFCGT
                     SISSQGMPQDRIDAARPFNPHIHLSRGDQRGSMLFTLDANRLQVALRVVGNVKDPASP
                     VRTGAEFAVEAGRPGALAG"
     gene            complement(28882..30909)
                     /locus_tag="PKV56_02840"
     CDS             complement(28882..30909)
                     /locus_tag="PKV56_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HOX66775.1"
                     /translation="MNQPPCTARRDRTAQDRFADIVERSPGRRELLRRGAGAALAALL
                     GPVLPAAAHSRTPSGPAAPKAFFDPVEASKADTVRVPAGYRVQVVYAWGDPIDGVGPA
                     FMPDGSNSAADQARQAGMHHDGGHFFSLPDWKAGSSTSGLWAVNHEYVDPSLLIGPPE
                     RWSSPEGVAKSKAAHGVSVVEFARDRSGRWAVVRNSRHARRITADTDIDIAGPARGDP
                     RLKTAADPQGTRVRGTLGNCAAGFTPWGTYLTCEENFNAVFGSDDVGFRPTADQRRYG
                     LSVRGAIGHAGPLYPWWKQDPRFDLAREPNEANRFGYIVEIDPYEPGRRPVKRTAMGR
                     FKHENCEWLATKDGRLAFYMGCDEANEYIYKFVTDGKFDPKRRDANRDLLDHGTLHVA
                     KFHDDGSGVWLELTPGRSGLSAEHGWSSPADIAIRTRQAADRAGATLMDRPEWVAVNR
                     RTGEVFATLTNNTRRGTLPASTHAVDGSSAGSSARPPTDRVNPRASNIYGHIVRWKDD
                     GGDPASLTFRWNVFVLAGDPAQADPARQGSIDGDLFGCPDGLWVDDTGMLWIQTDVSP
                     ARLPTGGPDSQDPEFGQFGNNQMLMADPSTGRIRRFLTGPVKCEVTGAHMTPDRKTMF
                     VNIQHPGEAGSDADLSVSPTRYSSWPDGAMGGRPRSATIAITRGDGRTIGS"
     gene            31162..>31666
                     /locus_tag="PKV56_02845"
     CDS             31162..>31666
                     /locus_tag="PKV56_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HOX66776.1"
                     /translation="MKGKSHPTNDVSLNPSSGHSIRDVIDHVDASRRQFIQSGVGAAA
                     LASAGGITLGGLVGTVQAAPVPAGLGFPGIGFESIPASIDIDPSSTASAVADLVKVPV
                     GYTANVFVAWGDPIMPGGAPFSGTASETAADQLKQFGMHNDGMHFFPIDDDRDDDKRR
                     DGHDGHDD"
     assembly_gap    31667..31766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31767..33560
                     /locus_tag="PKV56_02850"
     CDS             <31767..33560
                     /locus_tag="PKV56_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HOX66777.1"
                     /translation="DDDRDDDKRRDGHDGHDDDRGSSKRGILCVNNEYTHEEVLFTDG
                     QVGAGYSIAKTRKSQAAHGVSIVEARRKGGKWSVAKNSSLGRRITANTFMQVSGPAAG
                     HALMQTREFLITDVASTPTGNTTNGRVANGTVNNCAHGITPWGTYLTCEENWNGNFGG
                     TGAVDTSVTTEIGKLNRRYGLNAGGFGYRWHTTDPRWDLTTNPNEAHLFGWVIEIDPY
                     DPKSIPVKRTALGRFKHESAQYVVDKDDHVAFYLGDDERNEYIYKFVCKGKLKKGRRA
                     ANRNLLDEGTLYVARFDADLTGAWLPLTPASIGLDGKALRENANFTGADDAEVLAKIL
                     IKTRMAADAVGATMMDRPEWTGARPRINGFKEVEVYCTLTNNNRRGTAGASTNAADGS
                     TSAGSARPPVDAANPREDNVHGHIIRWREAGKSVKATAFTWDLFAQAGDTLTAKVAKP
                     TNNYQGNIVDAPNGSADYGAPDGLWFDYFGRLWVQTDQIGNGSGDFVNIGSNVMVCAD
                     PNTGETRRFLTSPNKCEVTGVTMTPNGRTMFVGIQHPGEDALAANPTEFSNWPQGQFA
                     VESDGVTALPGGRPRSSVVVITKDDGGIIGT"
     gene            complement(33659..34417)
                     /locus_tag="PKV56_02855"
     CDS             complement(33659..34417)
                     /locus_tag="PKV56_02855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX66778.1"
                     /translation="MIEKMPVAVVTGAGSGIGRACSLALLEAGYIVVLAGRNKTSLDA
                     TANAAGVNATHALAVPTDVSDPASVAALFERVRSICGRLDLLFNNAGTGAPAVPLDEL
                     SFEQWKTVVDVNLTGAFLCTQHAFRLMKAQTPRGGRIINNGSISAHVPRPYTTPYTAT
                     KHAITGLTRSTSLEGREHDIACGQIDIGNAATDMTAPMAAGILQANGSRMAEPRMDVA
                     HVANALIHMASLPLSANVQFMTIMATAMPYIGRG"
     gene            complement(34414..35235)
                     /locus_tag="PKV56_02860"
     CDS             complement(34414..35235)
                     /locus_tag="PKV56_02860"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOX66779.1"
                     /translation="MRLLTVPASRGVLWVRSGFRVFFGRPLAFAALFASFLFGAMLLL
                     LVPVIGTLLLLTALPLVSQGFMLATQRALAGQTPMAGVFIEPVRGRRPQAIAMLKIGL
                     IYSAASLFIMWFSNVVDGGTFDALQEAMTAKTADTERLDVLLNDPRLSAGMLVRLGLA
                     SLLALPFWHAPALVHWDGQGPAQSLFSSTIACWRNKGAFMVYGLTWAALVFLFALLAN
                     TVFMLLGAPQLVAVAALPAGLMFSTVFYASLYFTFTDCFEQRTAALAPPAAEPSP"
     gene            complement(35232..36236)
                     /locus_tag="PKV56_02865"
     CDS             complement(35232..36236)
                     /locus_tag="PKV56_02865"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOX66780.1"
                     /translation="MAVYTEVPFDTAAEFVSRLNIGRLTTLEGCSGGIENTNYFADTT
                     QGRYVLTLFERLTAEQLPFYLRLMKHLAQRGIPVPDPCADAGGEILHTLQGKPAAVVD
                     RLRGSSRLAPDVEHCRQLGAMLARMHLAGADFELHQPNLRGLAWWAETVPVVRPFLTE
                     SQGTLIEAELAFQQHLAQSAACKRLPRGPVHADLFRDNVMFDESAEGDDRLSGFFDFY
                     FAGVDTFLFDIAVCVNDWCIDLVTGRLDEDRALALVAAYDAVRPITGDEVRLFPAMLR
                     AGALRFWISRLWDLHLPRDAAMLTAHDPTHFERVLRQRADTPWHPPLPLSRDATPDAE
                     "
     gene            complement(36311..36712)
                     /locus_tag="PKV56_02870"
     CDS             complement(36311..36712)
                     /locus_tag="PKV56_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66781.1"
                     /translation="MLTSHRRLPALLALCCAASLHLVASAQERVPAAARPSASAVQPS
                     ARPLAPASTAAEEKTAQGEPAVRRTVIEDDGARIDELKVRGQTKRITVTPKTGGKPYE
                     IIPPSGAPDQTEGPNGSNGSVGKRVWPVLSF"
     gene            36819..39656
                     /gene="polA"
                     /locus_tag="PKV56_02875"
     CDS             36819..39656
                     /gene="polA"
                     /locus_tag="PKV56_02875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOX66782.1"
                     /translation="MSSTPLPVMMLVDGSSYLYRAYHALPDLRGPDGFPTGALHGMVA
                     MLQRALKDVNPEHAACVFDAKGPTFRDDWYPEYKAHRSPMPEPLVQQIEPIHEVVRLL
                     GWPVLVVPGIEADDAIGTLARVAAASGHQVVISTGDKDLAQLVNERVTLINTMSNERL
                     DVQGVMTKFGVPPERIVDYLTLMGDTVDNVPGVDKVGPKTAAKWIAEHGSLQGVIDAA
                     ASIKGAAGENLRRALDWLPQAQRLVTVRLDCDLAGHVPGWPGLDALALKEADKVALRD
                     FYARYGFKTWLRDIESQLGVAAAEPPARKRSVAARPASGEPDLFETEVENNSEVAGAA
                     APADPVEHRYDTVLTIEALDAWIAKIEAASRSGAVTAIDTETDSLDAMKARLVGISLS
                     VAPGEAAYIPLRHDYTGAPEQLSPVDVLARLKPWLEDEGAPKLGQNIKYDTHVLQNAG
                     IQVRGYVHDTMLESYVLEAHKPHGLESLAQRHLGRSGLSYEDLCGKGAHQIPFSQVDV
                     AKAAVYSCEDSDMTLDVHRTLWPLIDAEPGLRDVYARIELPTALLLQRIERTGVLIDP
                     ARLASQSQQLGERMVALESEAYELAGQPFNMGSPKQIGEILFVKLGLPVKKKTATGAP
                     STDEEVLAELAADYPLPARLLEHRSLSKLKGTYTDKLPLMINPDTGRVHTNYAQAVAV
                     TGRLASNEPNLQNIPIRTAEGRKVREAFIAPSGHVILSADYSQIELRIMAHISEDPGL
                     LKAFAEGMDVHRATASEVFGIEPASVTSEQRRYAKTINFGLIYGMGAFGLAQSLGIER
                     SAAAAYIDRYFQRFAGVKRYMDDTRESAREKGYVETLFGRRVYLPEINGGNGPRKAGA
                     ERQAINAPMQGTAADLIKLAMLAVQRVLDAEGRAARIVMQVHDELVLEVPDAELDWAR
                     AAVPAAMGSVASLKVPLLAEVGVGANWDEAH"
     gene            complement(39720..40799)
                     /locus_tag="PKV56_02880"
     CDS             complement(39720..40799)
                     /locus_tag="PKV56_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOX66783.1"
                     /translation="MSPPDPNRDSVLENKVFLIVLVAVSLGFAWILTPFFGSVFWGVV
                     LAILFTPLHRKLRNRLPDRPSLAALITLAAIVLIVLIPLAVIAASLAREGASVYARTQ
                     SGELDFGRYFEQIVLALPSWIKDLMNQFGFGNIAALKQKLTAAITQGTSLIATQALNI
                     GQITFEFIVSFSITIYLVFFLLRDGAALALRINAAIPLDEQDKRDLLRKFTTVIRATV
                     KGNVVVAATQGALGGLAFWFLGVTGALFWAVLMAFLSLLPAIGAGLIWLPVALYFLVT
                     GAVGPGLALIAYGVLVIGLVDNVLRPILVGKDTKMPDYVVLISTLGGMAAFGLNGFVI
                     GPVIAAMFMAVWDIYILARGRQSEP"
     gene            40952..43141
                     /gene="ppk1"
                     /locus_tag="PKV56_02885"
     CDS             40952..43141
                     /gene="ppk1"
                     /locus_tag="PKV56_02885"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOX66784.1"
                     /translation="MSTEPAQPANDSPVALTAPVDTAAVPATAAPAAVPQPGDPALML
                     GRETSILEFNRRVLAQALRADVPLLERLRYICIVSSNIDEFFEVRFADYLEASRLPNA
                     GVTDADVQAITAEAHRLIDDQYAAFNDQVMPLLQRAGIVILNHADRDEAQRDWVDNFF
                     QKQVRPLLVPIGLDPSHPFPLVANKALNFIVRLGGKDAFGRENTIAIVKVPRVLPRVI
                     RLPDALVGGKQGFVLLTSVIRAHLGELFPGREVETFSQFRVTRDSDLEVDEDDVKNLR
                     QALRSGLSSRHFGQAIRLEVVSSCPPELSEFLLEQFDLPEAALYKVNGPVNLVRLNQL
                     IDQVTDRSARPPLRFPPHEGVWPEQRLPRGQSIFASLRRGDVLLHHPFESFDPVVQFL
                     REAVNDPAVLAIKQTIYRTGSESPLMDLLIEAARRGKEVMVVVELKARFDEEANINWA
                     ERLEAVGAQVVYGVVGLKTHAKLLLVTRREVGTGGAVRLRRYVHLSTGNYNPKTARLY
                     TDVGYLTADAELTADADAVFQQLASLGKMKPLRQLLQAPFTLHRRLIHTIDRVADAAR
                     AGLPARIVVKLNALTDPPLIDALVRAGQAGADIDLIVRGACVLRPGVAGATDRIRVRS
                     VVGRFLEHSRVMYFRWGPGDDDEVLYLSSADWMSRNMQRRIEVAWPVRDAALRQRVID
                     ECLVPYLHDRREAWMLNSDGSFSRLGSNGASAQQALVARYKSGSRRT"
     gene            43146..43607
                     /gene="sixA"
                     /locus_tag="PKV56_02890"
     CDS             43146..43607
                     /gene="sixA"
                     /locus_tag="PKV56_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOX66785.1"
                     /translation="MDLILWRHAEAHIVKDGQTDLERALTAKGERQAQRMADWLNQRL
                     AHSTRILVSPALRCQQTAKALGRSFKTIDALSPDAAGDALLKAARWPESPDPVLVIGH
                     QPTLGLVAAHLLADTPQEWSIKKCAVWWLRHRDREGNTQVVLQAVQSPDCL"
     gene            complement(43621..45093)
                     /locus_tag="PKV56_02895"
     CDS             complement(43621..45093)
                     /locus_tag="PKV56_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOX66786.1"
                     /translation="MGSNSFRLELGQLDGTRYRRIDYLKETVRLGGGLDENGLLTEEA
                     MLRGLSCLSRFAHRLQGVAPWQVRAVATQTLREARNRNAFLARAETVLGYPIEVISGR
                     EEARLIYAGVARLQPSEQPRLVIDIGGRSTEMILGEGRKPLRAESFQVGSVSLSMKYF
                     GDGRYTQAAFRAAQVAAGAELEEALEPFAPTHWVEALGSSGTVGAVSQLLSANRVTDG
                     RITPGGLRWLMEQCLAAGRVDRLALAGLKEDRRAIIAGGLSILYTLATHFGIDALLPA
                     RGALRQGVIFDLDERLNAGQQPNDGEDVRDSSVRELQQRFLVEEAQAQRVGQVAEALY
                     NNVQPDAGRDARRELQWACALHEIGMMVSHHDHHRHSAYMLGNVDAPGFSQSQQRRLA
                     ELVLAQRGGLRKVEASLMQPDFAWQAMCLRLAIIKCHARGTVDNHAIALRRKGSVAEL
                     SWQPGWIEIHPRTHYLLTEEAAAWQRGGPLELELPRSSGY"
     gene            45334..46206
                     /locus_tag="PKV56_02900"
     CDS             45334..46206
                     /locus_tag="PKV56_02900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOX66787.1"
                     /translation="MFRNDVESLCPTRGYSRRDFVQTAVGSGFAVAVLPVGAQIIRTD
                     TAGLLAGPVTIPVGGFELPAYRAVPAQASAPPVVLVISEIFGVHEHIADVARRFAKLG
                     YLAIAPELFVRQGDAKAATDMARLISEIIAKVPDEQVMGDLDATVAWAKTQGGDTSRL
                     GITGFCWGGRITWLYSARNPAVKAAVAWYGRLVGAASALQPMHPVDIAAQLHAPVLGL
                     YGGADAGIPQASVDQMKAALSAGSPGARRSQFVVYPDTPHAFHADYRPSYRKPAADDG
                     WQRCLDWLRANGVG"
     gene            complement(46221..46655)
                     /locus_tag="PKV56_02905"
     CDS             complement(46221..46655)
                     /locus_tag="PKV56_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX66788.1"
                     /translation="MFKRILVPTDGSDITAKAIETAIGLAKSLGGQIVAMSVKEPFPY
                     NAMSEMQPVPPQEYFDAQQRISAARVQQVQQACAAAGVTCESHTVEADHPWQAIIDLA
                     KAAHCDLLVMASHGRRGVTALLLGSETQKVLTHSTIPVLVVR"
     gene            complement(46692..47564)
                     /locus_tag="PKV56_02910"
     CDS             complement(46692..47564)
                     /locus_tag="PKV56_02910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOX66789.1"
                     /translation="MNPSATLYTTLIAAGDLRSLIDSGAPVLILDASFDLADTAAGER
                     TFLEAHLPRAHHVHLDRDLCGQKTGRNGRHPLPEPDVFAATAGQLGITPTTQVIIYDR
                     QGAMFAARLWWMLRWLGHAPVAVLDGGFAAWQDSGGPVEAGAVAAVTPQPAYPVGGMA
                     SSPMRTLDAAALQSQLGGIALIDARAPERFRGDVEPLDAQAGHIPGARNRFFKENLAA
                     SGRFKPADELRTGFLALLGTRPPSEVVHQCGSGVTACHNLLAMTVAGLDGAALYPGSW
                     SEWSSDPTRPVARG"
     gene            complement(47561..48805)
                     /locus_tag="PKV56_02915"
     CDS             complement(47561..48805)
                     /locus_tag="PKV56_02915"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOX66790.1"
                     /translation="MSDLHVARGALTPGAGRPTRTGARHNALPLAPSTYFDDALFQRE
                     ADTLFRHGPRYLGHELSVPNPGDYYTLPQEGDGRVLVRTPKSVELISNVCRHRQALIL
                     HGRGNTHSNIVCPLHRWTYDLKGQLIGAPHFTESPCLHLNNYKTRSWNGLVFEDNGRD
                     IAADLAALGPRADLDFSGHVLDRVHVHECDYNWKTFIEVYLEDYHVGPFHPGLGNFVT
                     TDDLRWEMGSEYSVQTVGVATSSGEALGKPGSDVYSRWHNEVLRYQQEHHDGRPPKHG
                     AIWLTYYPGLMIEWYPNVLVVSTLYPRGPRHTTNVVEFYYPEEIAAFEREYMDTQQAA
                     YLETCIEDDEIALRMDQGRAALWQRGDHESGPYQSPMEDGMEQFHTWYREAMESDLQA
                     CPERPSAGMQRLLNSSTLNVEP"
     gene            49089..49355
                     /locus_tag="PKV56_02920"
     CDS             49089..49355
                     /locus_tag="PKV56_02920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOX66791.1"
                     /translation="MAKTSSTVANPVSAQAGTAALPDNYEAALSELERLVGAMEAGQL
                     PLDQLLDRYRRGAELLNFCRQRLEAVEQQVKVLEDGQLKPWNPT"
     gene            49355..50263
                     /locus_tag="PKV56_02925"
     CDS             49355..50263
                     /locus_tag="PKV56_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX66792.1"
                     /translation="MTSLTDFDSWARSELVAVEECLGLWLPADAPAGLGTAMRYGVLD
                     GGKRLRPLLVLAASHAVGGARIASLRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPT
                     VHVQFGEAQAMLAGDAMQALAFEVLTPASCDADARSIPPALQARLCGLLSRAAGHSGM
                     AGGQAIDLASVGRPLDERSLRDMHYRKTGALLRASVMMGAACGEVDAGGWAALEVYGD
                     SIGLAFQVIDDVLDVTQQSDVLGKTAGKDVDANKPTYVSLMGLGPARRHAFELRDQAK
                     SALSSSGLRDTVLLGLLADRVVERDS"
     gene            50266..52170
                     /gene="dxs"
                     /locus_tag="PKV56_02930"
     CDS             50266..52170
                     /gene="dxs"
                     /locus_tag="PKV56_02930"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOX66793.1"
                     /translation="MKNALLQSIQSPADVRRLSRSELHLLASELRDFLLHSVSQTGGH
                     LSSNLGTVELTVALHKVFDTPNDRIVWDVGHQTYPHKILTGRRDRMGSLRQLGGISGF
                     PRRDESEYDAFGTAHSSTSISAALGMAVGARMKGEHRKAIAVIGDGAMSGGMAFEALN
                     NAGVAGADVLVILNDNDMSISPPVGALNRYLARLMSGRFYANARDTAKSVLKNAPPLL
                     ELARRFESHAKGMVVPGTIFEEFGFNYVGPIDGHDLDALIPTLENLRNLKGPQFLHVV
                     TKKGQGYHLAENDPVAYHGPSRFNPAVGITPAAPGKPTFTQVFGTWLCDMAEADTRLV
                     GITPAMREGSGMVEFESRFPRRYHDVGIAEQHAVTFAAGLACEGLKPVVAIYSTFLQR
                     AYDQLIHDVAIQNLPVVFALDRAGLVGADGATHAGNYDIAYLRCIPNMSVMTPSDENE
                     CRQLLYTAFLQDHPTAVRYPRGKGVGVEVHQAMTAIPLGKGEIRRMASPSSGSHCRRI
                     AILAFGTLLHAALDAAERLDATVANMRFAKPLDIELVCELARSHDALVTIEDGSAMGG
                     AGSAVLEALQQAGLMMPVLVLGLPDEFIEHGDPAVLMSRCGLDSAGIEQSILKRFGGR
                     PALVRPAVHQ"
     gene            complement(52190..53185)
                     /locus_tag="PKV56_02935"
     CDS             complement(52190..53185)
                     /locus_tag="PKV56_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX66794.1"
                     /translation="MRWSQSDDVVTHAANLTGWQQQYDQLSRGPFHGELSELWFDEIQ
                     VFRERTSHAVRQTCQIRPDTIWCGITMAPDGSRIEGQVVGAGGVMVSGSGAEFELLTP
                     NQHEIFGIVASRSALKEAANTLGQPLNLAALSHPGWFACDPAAHAQLHRGLALLLAEA
                     GSGASSHDHAQSRRFAQSAVLDRLLSVLATPLVEGHEGLSFDRRRSLVRRACDQVRMH
                     PHAVPTVPELCEHLHVSRRTLQYAFETVVGSSPVDFLRTLRLNAARRDLLRGAATSVQ
                     DAAGRNGFWSLSQFASDYRRQFAERPSQTLARTRPDSRGLPAAGTSSAHATPTLQ"
BASE COUNT         8921 a        17295 c        17922 g         9116 t
ORIGIN      
        1 cagggccgtg cgctggtggg tcgacgggct gctcgatcgc accgacgatg gccgcatctt
       61 ggtgcgtaca ggcgagtcgc agctgctgat ggataccgac ccggataatc cggcggatgc
      121 accctaacgc cctcctagaa ctcgccaccg aactgctgca ccgggtgatg cagttcgact
      181 ctccggccga caacgtcgtc tccgatttct tccgtcagaa ccgtgggctt ggcatgcgcg
      241 agcgccacac gctggcggag accacctaca ccgtgctgag gcaacgcctg ctgttccagc
      301 atctggccca gtctggcaag ggtgagatgg agcggcgcct cgtgctgctg ggctggcagg
      361 gcaacgccgc tttcctgcgc gctgcgcttc aggagcatga agtgctgtgg ctggaacagg
      421 tcggcgccgt cgaccgcacg acgctgccgg aaaagctgcg tcacaacctg cccgagtggt
      481 tggccgatcg tctgcaggcc gaggtcggcg acgagttctg gccgctggtc gaagccctga
      541 acggcagcgc gccgctggac ctgcgcgtca acacattcaa ggcgaagcga gaaacggtgc
      601 aggccgagtt tgccgccgcc aacatcgagg ccttgcccac gccctattcg ccgtggggct
      661 tgcgcatcga aggcaagcct gcgctacaca agctcgaggt cttcctgcgc ggcgacgtcg
      721 aagttcagga cgagggcagc cagttgctcg ctctgatgac cgacgccaag cgcggcgaaa
      781 tggtggtcga cttctgcgcc ggcgccggag gcaagacact cgcgctcggt gcggccatgc
      841 ggaacaccgg tcgcctctat gcgttcgaca cctccgggca ccggctggcg tcgctcaaac
      901 ccagactcgc gcgcagcggc ctttcgaacg tgcacccggt tcagatcgcg cacgagcgtg
      961 acgatcgcat caagcggctg gccggcaaga tcgaccgggt gctggtcgat tcaccctgtt
     1021 cgggtctcgg tacattgcgt cgcaatccgg acctgaagtg gcggcagtcg ccgcagtccg
     1081 tcgaggaatt gaaggtgaag caggcggcga tcctggccag cgcgtcgcga ctggtcaagc
     1141 cgggtggccg tctggtctat gccacctgca gtctgctcca gggcgagaac gaggagatcg
     1201 cggaagcgtt ctcttccgag cgggtgcgcg atttcaagcc gctggcggct cacgatgtcc
     1261 tgagttccgc gcatgtcgct caggccgaca cgctggttcg cggtccgtac ctgcgcttgt
     1321 ggccgcaccg ccatggcacc gacggctttt ttgccgcggt ctggcagcgc gcttgatctg
     1381 ggtgtgactg gcctcaactg ctggggaatc agggggcgct cggcccgtta atcacctgat
     1441 tcagtcgagt gatcggcccc tacaataggt gggtttcgtt tgccacagtg tggaattgat
     1501 ttcatgaact tgttgatgac ggtttcggac ggtctcttga attggctcgc caacggcttg
     1561 gtccagacca cctggtggca gattgttctg tatgcattgg tggccacgca catcacgatt
     1621 gccgcggtca cgatcttcct gcatcgatcg caggcgcatc gtgcgctgga tctgcatgcc
     1681 attccgtcgc atttcttccg gctctggctg tggctcacga cgggtcaggt cacgaaggaa
     1741 tgggtggccg tccaccgcaa gcaccacgcc aagtgtgaaa cccccgacga tccgcacagc
     1801 ccgcagacgc gcggcatcaa gaccgtgctg ctgaccggtg cggagctcta tcgcaccgag
     1861 acgaagaacc aggaaaccct ggtcaagtac ggacacggaa cgccggacga ctggctggag
     1921 cgcaatctct acacccgcta cagctggcag ggtgtggggc tgatgctcct gatcaacctg
     1981 gctttgttcg gcgcggctgg actgaccgtc tgggctgtcc agatgatctg gattcccgtg
     2041 accgccgccg gcatcatcaa tggcctcggc cactggtggg gataccgaaa tttcgaggca
     2101 ccggacgcct ccaccaacat ctccccgtgg ggcatcatca ttggcggcga agagctgcac
     2161 aacaaccacc acacgtaccc gacgtcggcc aagctgtcgg tcaagcctta cgagttcgac
     2221 atcggatggg cttacatccg cacattggaa atgctgggcc tcgccaaggt gcgcaagacg
     2281 gcgccccagc tgaagctggg cgagatcaag cctgtggccg actcgaagac gctcgaggcg
     2341 atcatcgcca accgctacga agtgatggcc aagtacgccg ctcacctgcg agaagaatgc
     2401 cgcagcgagt tggcgcgact gaaggcggaa ggtggcgaaa attcggctcg ctggaaggac
     2461 atgcaccttg cgaagcgttg gctgcaccgt gacagcgacc ggatccccct cgtcgtgcag
     2521 gagcgcgtcg cgacggtgtg cgcgcagaac gacgtgcttt ccaagctggt gtccatgcgc
     2581 gaggaactgc gccagctgtg gacgcgtacc aatgtgtcgg ctgaccagct ggtggtcgat
     2641 ctccaggcct ggtgcaagaa ggccgaggac agcggaatca tcgcgctgca ggagttcgcc
     2701 ctgaagctgc gcgccgctca cgcctgacct tcaacgaggt gcagaccggt cctccgcagg
     2761 gatgaccggc aacaaaaaac ccgctggatt ccagcgggtt ttttgttggg tcggaagctg
     2821 ttatctcagc ttcgtttcct tgtacatcac gtgcttgcgg gctttcggat cgaatttcat
     2881 gatttcgatc ttctcgggct tcgtcttctt gttcttgtcg gtcgtgtaga aatgaccggt
     2941 accagccgaa gactccagct taattttctc gcgtccacct tttgccatga tccgctcctg
     3001 atttagattt cgccgcgggc gcgcaggtcg gcaacgacct tctcgatgcc caccttgtcg
     3061 atcaagcgca gcgctgcgtt agaaatgcgc aggcgcaccc agcggttctc ggtttcgagc
     3121 cagaaccggc ggtattgcag gtttggcagg aatcgacgct tggtcttgtt gttcgcatgg
     3181 gaaacattgt ttcccgacat cgggcgcttg cccgtgactt gacagacgcg tgccatgacg
     3241 ctgctccaat aggttcgtgt ttgaggatcg ctgatgcagc gcgccaaggg cgtgtgatgc
     3301 atcggggctt gcgcctgcgt aaccgctgca caacgaccct cgaaagatca aggtccccag
     3361 cacagcgaag cccgcgagta taaccggctt ccagcccaac aggccagaag cggtctccga
     3421 tcactgttct aggaagcgct gggagtccag ggcagccatg cagcctgttc ctgcactggt
     3481 gatggcctga cggtagacgt gatcctgcac gtcgcccgcg gcaaacacgc ccggcacgct
     3541 ggtcatcgtc gccatgccgt tgagtccgcc ccgggtgacg atgtagccgt ccttcatttc
     3601 caggtgtccg gcaaagattt cggtgttcgg ctggtggccg atcgcgatga agcagccttg
     3661 cagcgccagt tcggtcgtgg agttgtcctt tgtgttgcga gtgcgcacgc cggtgacgcc
     3721 ggaggcatct cccagaactt catccagcgt gctccacaga tggagcttca ccttgccagc
     3781 cgcgaccttg tccatcagct tgtcgatcag gatcgcttcg gccttgaact tgtcacgccg
     3841 atgaatcagg ttcaccgtgt ttgcgatgtt cgacaggtag agcgcctcct ctacggcggt
     3901 gttgccgcct cccacaacgc agacatcctg gccgcgatag aagaaaccat cgcaggtcgc
     3961 gcagccgctg accccgcgcc ccatgaatgc ctgctctgat ggcaggccga gatacttggc
     4021 cgatgccccg gtggcaatga tcagtgcgtc acaggtgtag cttcccgagt caccggtcag
     4081 cttgaaaggc cggtcggaaa aatcgacctg gttgatgtgg tcgaaaacga tctgcgtgtt
     4141 gaaccgttcg gcgtgcttct ggaagcgctg catcaggtcc ggtcccatca cgccatcgac
     4201 atcggccggc cagttgtcga catcggtggt tgtcatcaac tggcctccct gggcaatgcc
     4261 ggtgatcagc accgggttca ggttggcgcg ggccgcgtag atggcggccg aatagccggc
     4321 ggggccggaa cccaggatca ggacacgtgc gtgggtggat gtgatgctca tggcggggca
     4381 gtaaatggag aaaacccgat cagtttgaca tgccagcaac agcactggca gcagcgaatc
     4441 caatggcgac aatagagaaa gatcttttgt ccagtgaatt cctcacacgt cctgccgata
     4501 acgccgataa cgtccacaaa ggagctccac gatggcgatg ctttcgaatc tcgacctgat
     4561 ccggcgcgtg ccgatctttt cgctgctcac caacgaccag gcacagggaa tcgccgacag
     4621 tgtggtcaag cgtcgctacc ggcgcggcga gatcatcgtc gagcacggtc gcaagtcgaa
     4681 tgccctgttc atcctgctga cgggtcgggc gcgcgtgctc acagccgact cgcggggccg
     4741 tgaggtgatc cttgccgtgc tgcagtccgg cgattacgtc ggcgagatga gcctgatcga
     4801 caacgaaccg cattccgcca ccgtacgcgc cgagatacag accgacatgc tgattctcgg
     4861 tcggcccgag tttgcccgct gcctgccgga gaactccagc ctgtcctacg cgatcatgcg
     4921 tggcctcgtg gcgcgcctgc gcagtgcgga ccggcagatc gagtcgctgg cgctgctgga
     4981 tgtctacggg cgagttgcac ggtcgctgct ggacatggcc gaagtcatcg gtgacgcgaa
     5041 gatcatccgc aacaaggtgt cgcgtcagga cctggccaag gtggtgggag cgtctcgcga
     5101 gatggtcagc cgcgtcatga aggacctcga ggagcggggc atgatcgaga cgcaggaaaa
     5161 cggttcggtg gtgctgaagg aacgactgac ctgagccttc ctcccgaccg gggtcgcctc
     5221 ggtcgtcagc gacattccgc cacaatgagc ggatgacatt tccgatcggt tcgctccgct
     5281 ctgccgcggc agattccact gctgctcacc gtgcctcggt ggccggtgat tctcgtgatc
     5341 gcgaggtcgg cgctgctgca tccggtaccg gatcgatggc gtcgacctgg cctcgtcagg
     5401 cccgcctgct cctcggcggc gtggtctggc tgctgatgct gatcgcactc gtcacgcaca
     5461 gcccccagga cgcagccttc tcgacttcgg gtgcgggcga gctggcgctc aacaaggccg
     5521 gtgccatcgg tgcctggctg tcggatgcag ctttcttcgt gttcggtttc tcggtgtggt
     5581 ggctcatggc ggtggctttg caccggtggc tcggggcgct ggccggcctg ctgcgaggcg
     5641 cagatggcga ggcagcccac ctgtcaccga tgcgtcgggc ctggccgctg tgggctttct
     5701 ggctcggact cgcgctgctg atgtcggcga gcaccgcact cgaatggacg cgcatgtacc
     5761 aatgggagcc ccgccttcct ggcagccatg caggcggcgt gctgggttac ctgctcgggc
     5821 cgctgagcat gaagtggctg ggcttcgcgg gttcgggcgt gctgtggatc gccgcgatgg
     5881 tggtcggtct gtcgctgtcc ttccgctttt cctggttgca ggtggccgat gcgattggtg
     5941 gccgcctcga cgcctggcgg gatcgtcgtg tcgagcgcat cgagatggcc gaggacttcc
     6001 gcctcggcga gcaggctttg cgcgagcgcg aggacgtggt cgaggtcgag cacgccctgc
     6061 acgaggacca tgcccccatc gtcatcgaga ccccggtggt cgacgtgccg aagagcgagc
     6121 gcgtggtcaa ggagcggcag aagccgctgt tcatcgagct tgccgacacg aaattgccgc
     6181 aggtcgatct gctcgacgct gctccgggcc gcaccgagac ggtgtcgccc gagacactgg
     6241 agatgacctc gcggctgatc gagaagaagc tgaaggactt cggcgtcgag gtgcgcgtgg
     6301 tcgcggcctc gcccggcccg gtgatcacgc gctacgaggt cgagccggcc accggcgtga
     6361 agggcgcgca ggtggtgaac ctggcgaagg acctggcgcg cagcctgagc ctggtgagca
     6421 tccgcgtcgt cgagacgatt cccggcaaga cgacgatggc cctcgaactg cccaatgcga
     6481 agcggcagat gatccgcctg tccgagatcc tcggctcgca ggtctacaac gacgcggcct
     6541 cgcaactcac catgggcctg ggcaaggaca tcgtcggcaa cgcgatggtg gccgacctcg
     6601 ccaagatgcc gcactgcctg gtggccggca ccaccggctc gggcaagtcg gtgggcatca
     6661 acgcgatgat cctgagcctg ctctacaagg ccgaggcgcg cgatgtgcgc ctgatcctga
     6721 tcgacccgaa gatgctggag atgagcgtct acgagggcat cccgcacctg ctgtgcccgg
     6781 tggtcaccga catgaagcag gccgccaacg cgctgaactg gtgcgtgggc gagatggagc
     6841 gccgctacaa gctgatgagc aagctgggtg tgcgcaacct ggccggctac aacaagaaga
     6901 tcgacgacgc tgccgaacgc ggcgagacga tgggcaaccc gttcagcctg actccggaag
     6961 cgccggagcc gctcgagcgc ctgccgcatg tcgtcatcgt gatcgatgag ttggccgatc
     7021 tgatgatggt ggtcggcaag aagatcgagg aactgatcgc acgtctcgcg cagaaggcgc
     7081 gggcggccgg tatccatctg atcctggcca cgcagcgccc cagcgtcgac gtgatcaccg
     7141 gcctgatcaa ggccaacatc ccgacccggc tgagtttcca ggtcagcagc aagatcgaca
     7201 gccgcaccat cctcgaccag cagggcgccg aagccttgct gggcatgggc gacatgctct
     7261 acatgccgtc gggcaccggc ctgccgatcc gcgtgcacgg agccttcgtc agcgacgacg
     7321 aagttcatcg cgtcgtcgcg tacctgaaga gccagggcga gccgaactac atcgaaggca
     7381 tcctcgaagg cggcttactc gatggcgatt ccggcgagat cgcggtcaac gacggcggcg
     7441 gtcccggtaa cgagagcgac gcgatgtacg accaggcggt ggccatcgtg ctgcagcaca
     7501 agcgcgcgtc gatctcgctg gtgcagcggc acctgcgcat tggttacaac cgcgcggcgc
     7561 ggctgctgga acaaatggag aaatccgggc tcgtatcgtc catgggtacc aacggcaatc
     7621 gcgacatcat cgtcccggca cgcaccgaat gaagcgggac tgcgtggttc gtgcggccgc
     7681 atggctggct tgcgcgctgt tcaccgccgc accgttggcg gcccgtgccg acgcggtcga
     7741 agcgctgaag tcattcgtgc aggacgtcaa gaccggccgc gccaccttca cgcagaccgt
     7801 cacctcgccc gatgggggca agacgaagac ctcgagtggc agtttcgagt tcgcccggcc
     7861 cgatcgcttc cgcttcgcgt acaccaagcc gttcgagcaa ctgatcgtcg ccgacggcca
     7921 gaaggtctgg atccacgacg tcgacctgaa tcaggtcagc agccgaaagt tctcgcaggc
     7981 gctgggtgcg acgccggcgg ctttgctggc cggtggtgat ctggcgcgcg acttcgacct
     8041 gctgccgctg ccggcaaagg acggcctgga atgggcgaaa gccacgccga aggccaggga
     8101 cggcgccttc gaatcgatga gcatcggatt ccgcggcaag cagctcgctg cggtcgagat
     8161 cgtcgacaac tttgcgcagc gttcgaggct gactttctcg cagttcactg ccaacgccga
     8221 gattgcgcca caagccttca agttcaccgt cccgaagggt gccgacgtcc tggagcagtg
     8281 acttggcaag cgcaccgcga ctgccacaat ccgtcgcatg acgtcgctgc ccttgccgct
     8341 ggaagacacg gtacctgccc cggcgcccgc agcctccacg gcggacacca cgcaacccct
     8401 tgcagagcgg ctgcgtcccc acacgctggc cgaagtgatc ggccagaagc acctgctggg
     8461 ccccggcaag ccgctgcgct cggcgttcga ttcgggccgc ctgcattcga tgctcctgtg
     8521 gggccctcca ggggtcggca agaccacgct ggcgcggctg atgaccgacg cgttcgacgc
     8581 ccagttcatc gcgatttcgg cggtgctcgg cggcgtcaag gacatccgcg aagcagtcga
     8641 gcgcgcgcag gcggttcagc cgagcggccg gcgcaccgtg gtgttcgtcg acgaggtgca
     8701 ccggttcaac aaggcgcagc aggacgcctt cctgccgcac gtcgaatcgg gcctgttcac
     8761 cttcattggc gcaaccaccg agaacccgtc tttcgaggtc aactccgcgc ttctgtcgag
     8821 ggccacggtg catgtgctga agagcctcga tgcggccgat ctggtcgaga tgctcgctcg
     8881 cgctcgtcga cttctctctt cgccggccat gtcggacgct gcgcgcgacc ggctggtggc
     8941 ctatgcggat ggcgatgcgc gccgcctgct caacgcctac gagaacctgg tcgagatgac
     9001 gtccgagcag cccggactga tcgagatcga cgaggccctg ctcgaacgct cgctcggcga
     9061 acagctgcgt cgctacgaca aaggcggcga gcagttctac gacaccatct ctgcgctgca
     9121 caaggcagtg cgcggcagcg atcccgatgc ctcgctttac tggttcgtgc gcatgctcga
     9181 cggcggtgtc gacccgcgtt acgcggcgcg gcgcctcgtg cgcatggcga gcgaggacat
     9241 cggcctcgcc gatccacgcg ctcttcggat gacccttgat gccgccgaga cctacgagcg
     9301 gctcggctcg cccgaaggtg aactggcgct ggcgcaggcc gtggtctatc tcgccgtcgc
     9361 gccgaagtcg aacgctgtct atcgggccta caagagcgtc agggccttca tcgctcagga
     9421 cggaacccgt gcggttccgc tgcggctgcg caatgcgccg acacgcttga tgaaggacct
     9481 cgaatacggg cgaggctacc ggtatgcgca cgacgaggaa ggcggtttcg ctgccggcga
     9541 gcgctactgg cccgacgatg tcgacgggcc gcggttctac gaaccggtgc cccgcgggct
     9601 cgagatccga atcggcgaaa agctcgatga actgcgtcgc ctcaacgatg cggccacgaa
     9661 gaagacctga cgccatcccg gccaccggac accgacgaaa gcgccaaccg cgatggacac
     9721 cttcatccag cagatcatca atggtctggt gctcggcagc gtctacgcgc tggtggcact
     9781 gggctacacg atggtctacg ggatcatcaa cctgatcaac ttcgcgcacg gcgaggtgtt
     9841 gatggtcggt gcactgacca gctggaccgt tgtctcggcg ctggcggaca gcgggttgcc
     9901 gggctggctg atgctgctga tctccttgat cgccgcgatg gcggtgtgcg ctgcgctcaa
     9961 cttcggaatc gagaagatcg cctaccggcc cctgcgaagt gcccctcgcc tggcgccgct
    10021 gatcaccgcg atgggcgtga gcctgctgct gcagacgctt gccatgatca tctggaagcc
    10081 gaacccgaag ccttacccca tcctgcttcc gagcgagccg ttcaacatag gcggcgccgt
    10141 catcaacacc acgcagatcc tgatcctggt gaccaccgcg gtcacgctcg ctgggctgat
    10201 gatcctcgtc aatcgcacga agctcgggcg cgcgatgagg gccaccgccg agaatccccg
    10261 cgtcgccgcg ctgatgggcg tgaagcccga catggtcatc tcggccacct tcgtgatcgg
    10321 cgcggcgctg gcggctctgg ccggcgtgat gtatgccgcc aactacggat cggtgcagca
    10381 cagcatggga ttcctgccgg gcctgaaggc gttcaccgcg gcggtgttcg gtggcatcgg
    10441 caacctggcc ggcgcgatgc tcggcggcgt gctgctgggg ctgatcgagg ctttgggcgc
    10501 cggttacatc ggtgcgctga ccaacaacgt gctgggcagc cagtatcagg acatctttgc
    10561 cttcatcgtg ctgatcctgg tgctgacgct gaggccgcag ggactgctcg gcgaacgggt
    10621 ggccgaccgt gcgtgacgca gcagctgccc gtctgagaac ccgcgcatga gctcgtcacc
    10681 gccacgcgga aaacaattcg ggttgatgct ggtcgcagtc atcgccctgc tgatcctgcc
    10741 gctgatcgct cagcagttcg gcaatgcctg ggtgcgcatc gtcgataccg cactgttgta
    10801 cgtgctgctg gccctcgggc tcaacatcgt ggtcggctac gccggcctgc tcgacctggg
    10861 ctacgtggcg ttctatgccg tcggtgccta catgttcgcg ctgcttgcca gcccccacct
    10921 gaccgagaca ttcccggcga tcgcagcgat gtttcccaac ggcctgcaca tgcccatctg
    10981 ggtggtgctg ccgctcggtg cggcgttggc cggactggcc ggcgtgctgc tgggctcgcc
    11041 gacgctgaag ctgcgtggcg actacctggc catcgtgacg ctgggtttcg gcgagatcat
    11101 ccgcgtgttc ctggccaacc tggagcagcc cctcaacatc accaacgggc ctcgcggcat
    11161 cggacagatc gactcgctgt cgttgttcgg atgggacctg ggcaagaaat gggaactgtt
    11221 cggcttcgaa gttccgtcgg tcaccaacta ctactacctg ttcctcgcgc tggtgatcct
    11281 ggccatcgtg atctgtcacc ggctcgagcg aagtcgcatc gggcgtgcct ggatggcgat
    11341 ccgtgaggac gagatcgcgg cgagggccat gggcatcaac acgcgcaatc tgaagctgct
    11401 cgcgttcggc atgggagcca cgttcggtgg tgtcgcaggc gtgttgttcg cgaccttcca
    11461 gggtttcgtt tcccccgagt cgttctcgct gcaggagtcg gtgatgatcg tcgcgatggt
    11521 cgtgctcggt ggtctcggcc acattcccgg cgtggtcgtc ggtgcgctgc tgctggccgc
    11581 gctgcccgag gtgctgcgct acgttgccgg accgctccag gtcatgacgg atggccggct
    11641 cgatgcgtcg atcctgcgcc agttgctgat cgcactggcc atgatcggca tcatgctggc
    11701 gcgcccgcgc gggctgtggc cggctcccga gcacggtcac ggcgctcggg ctgaatccgg
    11761 tgagcggggt ggggtgcccc atggctgaag ccgtgttgaa ggtggctggc gtctcaaagc
    11821 gtttcggtgg ccttcaggcg ctgtccgatg tcggcatcac gatcgagcgc ggcatgatct
    11881 acgggctgat cggcccgaac ggagctggca agacgacttt cttcaacgtg atcacggggc
    11941 tctacgcgcc ggactcgggc agcttcgaac tggccggcca gccttaccgg ccgagcgccg
    12001 tgcacgaggt cgcgaaggcc ggcatcgcgc gcaccttcca gaacatccgg ctgttctccg
    12061 acatgaccgc gctcgagaac gtgatggtcg ggcgacatgt gcggtcgaac tcgggactca
    12121 tcggcgccgt ctttcgaacc gcggccttca aggccgaaga gcaggccatc gcgcagcgct
    12181 cgcaggagtt gcttgattac gtcggcatcg gcaagtacgc cgacttcaag gcgcgcacgc
    12241 tgagctatgg cgatcagcgc cgactggaga tcgcacgtgc gcttgccacg gatccgaagc
    12301 tgatcgcact cgatgagccg gcagccggca tgaatgcgac ggagaaactc gtgctgcgcg
    12361 agctgatcga ccgcatccgg cgtgacaacc ggaccatcct gctgatcgag cacgacgtga
    12421 agctggtcat gggcctgtgc gaccgggtca ccgtcctcga ctacggcaag cagatcgccg
    12481 aaggaacgcc ggccgaggtg cagcgcgacg gcaaggtcat cgaggcctac ctcggcgcag
    12541 cgcacgcgca gaactgatcc gcaacccctg aaagagtaaa tggccagaac gctgctcgcg
    12601 gtgcgtggac tgaaggtcgc ctatggtggt atccaggcag tcaagggcgt cagcttcgat
    12661 gtgcaggaag gcgagctcgt cagtctgatc ggtgccaacg gcgccggcaa gacgaccacg
    12721 ctcaaggccg tgaccggcct gcagccgatg accagcggag acatcgagtt catgggtcag
    12781 tcgatccagg gacgaggccc gtgggagctg gtgcggcagg gcctggtgat ggtgccggag
    12841 ggacgcggta cgttcgcccg gatgacgatc accgagaacc tgcagatggg tgcgttcacc
    12901 cgcgacgaca aggacatcgc tgccgatatc gaccgggtgt tcgccatctt tccgcgcctc
    12961 agggagcgca gcagtcagct cgcaggcacg atgtcaggcg gggagcagca gatgctggcc
    13021 atgggccgcg cactgatggc ccggccgaag gtgctgctgc tcgacgagcc gtcgatggga
    13081 ctgtcgccga tgatggtcga caagatcttc gaggtcatca acgacatcca caggcagggt
    13141 gtcaccgtgc tgctcgtcga gcagaacgcc agccgcgcgc tgcaactggc gagccgcggc
    13201 tacgtgatgg agtcgggcga gatcacgatg aacggcgatg cgaaatcgct gttgaacgat
    13261 ccgaaggtcc gggccgccta cctgggcgaa tagtcaggcc gcgccttcgg gccgcgccgc
    13321 ggtgccggtg gtcttgtcgg ccgcgctcag gaacgcatcg gcgtggaact catcctgcgg
    13381 cagaccgcag cgctcgacga agtcacgacg tgccgcatcc accatcgcgg gcgtgccgca
    13441 cgcatagacc tggtggccgg acaggtccgg caggtcggcc atgaccgctt cgtgcacgaa
    13501 gccggttcgt cccgtccagt ggtccgaggg aagtgcctcg gacagcacgg gaacgtaacg
    13561 cagctggggc agctcgcggg ccagttccag tgccgcctcg tgctggtaca ggtcggcccg
    13621 ggtccggcag ccccagtaca gcaccacggg tcggtcgctg cgcttgaaga tcagctgttc
    13681 gatcaaggcc ttgatcgggg catagccggt gcccgaggcg agcagcacga tgggcttcgg
    13741 tgactcctcg cgcaggaaga agctgccgaa tggtccttcg aggcgcagga tgtccttctc
    13801 cttcatggcg ccgaacacct ggtcgctgaa gagtccgccc ggcaggtggc ggatatgcag
    13861 ttcgatccat ggcttgtcgc cctgggtgtg cggtgcgttg gccatcgagt agctgcgacg
    13921 tgcgccgtcg cgcagcagca cgtccacgaa ctggcccgcg tgatatcgca aggcttcgtt
    13981 ggccggcagc tggagcgtga tgatcgcgac gtcggccgag ggcttggcga tgcggctcac
    14041 gcgtgcaggc atgcgccgaa ccggcatctc gccgagaccc gcgacagtgc gtgcttccag
    14101 ggtcacgtct gtctgcggcg tggcgcagca ggtgagggcg tagccgtttt cttcctcggc
    14161 gacgctcaac gcactgagct gatgtgcgcc atggatcacg cgaccttcga tcagccggca
    14221 cttgcaggag ccacaggcgc cgtccttgca gccgtagggc aggccgatac cggcgcgcaa
    14281 tgccgcggtg aggatgggct cgtcgcgctg gacggggaag ctgcggccgc tgggctgcag
    14341 cgtgacggtg atgctcatgg tggcgacggg cgaaacgtaa actgaggcgc gattctgccc
    14401 gccctcgtcc gatgacgttg tccatgcctg cacttccctc tgctttcaag cttcccgtgc
    14461 tgctgatcgt cggttgcggg gacgtcggca tgcgggtgct gcgcctgctc gaggggcgct
    14521 atcgcctgct ggcgctgact tccagtgctg cgcgcgtcga atccctgcgc gcagccggtg
    14581 cggtgccgct gcagggcaat ctcgatgatc cggcgacgct ggcgcggctg gcagggctgg
    14641 ccgatgccgt gctccacctg gcaccgcctc cgctgcacgg acgcaccgac ccgcgcaccc
    14701 gcaatctgct ggctgcgctc gcccgcaagg gccgtgtgcg ctgcatcgtc tatggcagca
    14761 ccagtggcgt gtacggcgac tgcggggggg cgcgcttcaa tgagacgcgt gcggtgaacc
    14821 cggcgactga tcgggcgcgt cggcgggtcg atgccgagac ccgccttcgc tggtatggcc
    14881 ggtcgcatgg cgcgcgcgtg gcgatccttc gcattcccgg catctacgcg aaggatcgcg
    14941 aaggcggcca tccgcgcgaa cgtctgctgc gcggcacggc ggtgctgcgg gccgaggacg
    15001 acgtctacac caaccacatc catgccgatg acctggcccg cgcctgtgtg gccgcgctga
    15061 cgagggcgtg gccgcagcgc gccatccatg ccagcgatga caccgacctg aagatgggtg
    15121 actacttcga tctcgcggcc gagctctgcg gtctggagcg cccgccccgc atcacacggg
    15181 acgaggcgcg cgaacgcctg tcaccgatgc tgctgagctt catgagcgag tcgcgccggc
    15241 tcgacaaccg aagattgaaa cgcgaacttc ggctgaaatt gcgctatccg acggtgagtg
    15301 acggaatctg agccgtggtc gaggggcgcg gtgttcagcg cccgcgcccg aacggcgact
    15361 gcccgcgcca gtagcgcagc atcaggaatc cgccgagcat gccgccgagg tgcgcgaaat
    15421 gcgcaacgcc cgatgcgctg ccggtcacgc cgagcagaag ttcgagtccg ccgtacaacg
    15481 cgacgaacac cttggccttc atcgggatcg gagggaacag cggcacgatg gtgcggttcg
    15541 gaaacatcat cccgaaggcg agcagcagcc cgaacaggcc gcccgaagcg ccgaccgtcg
    15601 gaaacgtcga gccgagcagc gcggtgatca gcaactgtgc tgcagctgcg gccagcacgc
    15661 tggcggtgta gaagtggatg aatcgcttcg ggccccagac ccgttcgagc tcggagccga
    15721 acatccaaag tcccagcatg ttgaagaaca ggtgaccgag accgccgtgc aggaacgcgt
    15781 aggtgaccac ctgccagggc aggaagccgt gccccaccgg ccacagcgcg agcaggcgag
    15841 tgaaccaggg cccgaagaag ccgtcgatgc agaacgccgc gatgttgatc agcagcaggg
    15901 cttgggtgat gggaggcatc gggggcatgc gcgagatcct gtgttcgatg ggctgtgcga
    15961 gcgatatgcg agcgtaaccg gacttgattc cgcctcgatc ccacaccacc gcgcaggggc
    16021 ggtgctccgc caagggggct gtggaataat ccgcttctgc gctgggcctc tgtggcggaa
    16081 ttggtagacg cgccggactc aaaatccggt tcttaacgga gtgtcggttc gattccgacc
    16141 ggaggcacca accgactttc tcttgtttgc cgatgcggtg caatgccgca tccacggcag
    16201 cagaccgacc gctcacccat acaccgtggc atggccaaga agcagccgat ctaccgctcg
    16261 ctggaagacc tgatcggcaa cacgccgctg gtacggctgg ttcgcctgcc cggcgccgac
    16321 gccgagcgtc gcggcaatgt gatcctgggc aagctcgaag gcaacaaccc ggcagggtcg
    16381 gtcaaggacc ggccggccat cagcatgatc cgtcgcgccg aagagcgcgg cgacatcaag
    16441 cctggcgaca cgctgatcga ggccacctcg ggcaacacgg gcatcgccct ggcgatggcc
    16501 gcggcgatcc gcggctaccg catgctgctg atcatgccgg aggacctgtc catcgagcgc
    16561 gcgcagacca tgcgggcctt cggcgccgaa ctgatgctga caccgcgctc cggtggcatg
    16621 gagcacgctc gcgatctggc cgaccagctg cagcgcgacg gcaagggcgt cgtgctcgac
    16681 cagttcgcca atggcgacaa cccgcgcgtg cactacgaga ccaccggccc cgagatctgg
    16741 cgcgacacgg gcgggcagat cacgcacttc gtcagtgcga tgggaaccac cggcaccatc
    16801 accggtacct cgcgctacct gaaggaaaag aacccggctg tccgcatcat cggagcgcag
    16861 ccgagcgagg gttcgcgcat cccgggcatc cgcaagtggc ccgaggccta cctgccgaag
    16921 atctacgatc cgacgcacat cgacgagacc gcttccgtca gtcaggcaga tgccgaggag
    16981 atggcccgac gcctcgcgcg cgaggaaggc atctttgccg gcatctctgc ggcgggtgcc
    17041 tgctgggtcg ctctggagct ggcgaagacg gtcgagaacg cgaccatcgt gttcatcgtc
    17101 tgcgatcgtg gtgaccgcta cctgtcgacc ggtgtgtttc ccgtctgatc cctagaatca
    17161 gcgacgcaca gcggagcgca cccgatgatc ctgatccaga aagaactcga cacccgcggg
    17221 ctcaattgcc cgctgcccat cctgaaggcc aagaaggccc tggcggacat ggcgagcggc
    17281 gaagtgctga aggtcgtgtc gaccgacccc ggatcgatgc gcgatttcca ggcgttcgcc
    17341 aagcagaccg gcaacgaact ggtggatcag tccagcagcg agaccgagtt cgtgcacctg
    17401 ctgcgcaggc gctgatcctg gcaagtcaga gctcgagttt cttcaggtac tcgcggaatc
    17461 ccgggcccag tttcgggtgg gtgagagcca gctcgacgtt ggcctcgagg aagccttcct
    17521 tgctgccgca gtcgtagcgc cgcccgtcgt agcgaaacgc gaagaccttc tcgcggcgca
    17581 tcagcgcggc gatgccgtcg gtcaactgga tctcgccgcc aacgccgcgc ggcaggccgg
    17641 caatctcctt gaagatcccc ggcgtcagga tgtagcggcc cgccacgccc agccgcgacg
    17701 gcgcgtcctc cggagcgggc ttttcgacga tgtgcgtcat gtccatcatg cgatcgttga
    17761 ccggcgtgcc ggccaccatg ccgtagcgcc gcgtctgatc gtcgggcact tcctgcaccg
    17821 cgatgatcga tgcctgccaa tccgcgaacc ggtcgaccat ctgcttgagc accgggggct
    17881 tgccgaccat caggtcgtcg gccagcagca ccgcaaaggg ttcgttgacg accacgcact
    17941 gtgcgcacag caccgcgtgg cccagtccga gcgccttcgg ctggcggatg tagatgcact
    18001 ccatgtcgtc gggcttgatg gagtgcacca ggtccagcaa ttcgtgcttg ccagcggcct
    18061 cgagctcggc ctccagttcg taggccgtgt cgaagtgatc gggaatcgcg cgcttgttgc
    18121 gcccgttgac gaagatcatc tggcggatgc cggcggcgta tgcctcctcg accgcgtact
    18181 ggatcagcgg cttgtccacg accggcagca tctccttcgg ctgcgccttg gtggctggca
    18241 ggaaccgcgt gccgaggccg gccaccggaa agatggcttt gttgatcttc atctgcgcac
    18301 tcctgttgtc tcagctgttg aaccgcttct caaatgattc tcacacgcga aaaagacaac
    18361 tccgtgcagg acggccgatg cgtcacgtaa cggtttgatt cccgtgcccg atggagtcag
    18421 gccgtggtcg aaagccggtg tacctgcgcc tggagtcgat ctcgcgtggc ggtgaattcg
    18481 gccattcgcg cctgttcctg agccacaacc gcagccggag cgcgggcaac gaagctttcg
    18541 ttggacaatt tggcggttcc cttcgtgatc tcgccttcca gtcgggtgat ttccttgcgc
    18601 agccgctcgg tctccgcggc gacgtcgatc tcgacgtgca gtgcgagccg tgttccgccg
    18661 aactcgacga ccggtgcatt gagtgtggcc tgcgcgtagg ccgcttcgtc ggtgtgaatc
    18721 tgcacctccg agaccttggc cagtaccttg agcagcggcg tggcagccaa gacaaaggcg
    18781 ccgtcgccca gtgccatcaa cggaacccgc ttgtccggag acaggctcat ctcgctgcgc
    18841 aggcgccggc aggccccgac catgcccttg agttgcgcga tccaggcatc ggccgtcgca
    18901 tcgatgcgct cgagctgcgg ttgggggtaa cgcgcagtga ccagactgtc tgccgagccc
    18961 gcagccttgc gcccggcgac cactgcgacc cgctcccaca gttcggcggt gatgaagggc
    19021 gtgatcgggt gcagcagccg caacacggtt tccagcacgc ggatcagcgt gcggcgcgtg
    19081 gcgcgctgcg ccgattcgtc gccggtctgg atctgcacct tggcgatttc caagtaccag
    19141 tcgcagtact cgtcccacac gaacgaatag atcgagttgg cgacgttgtc tagccggtaa
    19201 tcggcgaagc cctgctccac agcggcctcg acgcgctgca gttcgctggt gatccagcgg
    19261 tcggcctgcg agaagctcag gtacttgtct gccgatccgc ccggttcgca ctgtgtcttc
    19321 gtgtgctcgg ccaggccgca atcctgacct tcacagttca tcagcacgaa gcgagtcgcg
    19381 ttccagagct tgttgcagaa atttcggtag ccctcgcagc gcttgctgtc gaaattgatg
    19441 ctgcggccga ggctggccag cgaagcgaag gtgaagcgaa gggcatcggc gccgaaggcc
    19501 gggatgcctt cggggaactc cttttcggtc tccttgcgca cgcgcggtgc cgtctcgggc
    19561 ttgcgcagac ccacggtgcg cttgtccagc aggggcgcca gcgatatgcc gtcgatcagg
    19621 tccaccgggt cgagcacgtt gccttccgac ttgctcatct tcttgccctg cgcatcgcgc
    19681 accaggccgt ggatgtagac gtggtggaac ggcactttcc cggtgaagtg cgtcgtcatc
    19741 atgatcatcc gggcgaccca gaagaagatg atgtcgtagc cagtgaccag cacgctcgat
    19801 ggcaggaaca ggtccagttc cttcgtcttc tcgggccagc ccagggacga gaacgggatc
    19861 agcgccgacg agtaccaggt gtcgagcacg tcggggtcgc gcgtcagttc gccgccgtaa
    19921 ccggccgcag cggccttcgc gcgcgcctcg gcttcgttct tggcgacgaa cagctcgccg
    19981 tccgctccgt accaggccgg gatctggtgc ccccaccaca gctggcgcga gatgcaccag
    20041 tcggtgatgt tgttcatcca ctggtcgtag gtgttgaccc actgctcggg aacgaacttg
    20101 accgcgccgc tctcgaccgc gtcgatcgcc ttctgcgcga tgctcctgcc gtccggtccc
    20161 gcctttgtca tcgcgacgaa ccactggtcg gtcagcatcg gctcgatcac ctggccggtg
    20221 cgcgcgcagc gcggaaccat cagcttgtgc ttcttgacct cgaccaggaa accctgcgcg
    20281 tcgaggtcgg cgacgacgcg cttgcgagcg acgaagcggt ccagcccgcg gtaggcctcg
    20341 ggcgcgttgt cgttgatcac cgcatcgagc gtcagcacgc cgatgatcgg caggccgtgg
    20401 cgttggccga ccgcgtagtc gttgacgtcg tgcgccggag tgaccttgac gacgccggtg
    20461 ccgaacgcgc gatcgacgta gtcgtcggcg atcaccggga tcgtccgccc gcacagcggc
    20521 agcgtgaccc gcttgccgat cagggcgctg tagcgctcgt cttccggatg caccatcacc
    20581 gcgacgtcgc cgagcatcgt ctcgggccgc gtggtggcca ccaccacatc acccgagcca
    20641 tcctccagcg gatagcggat gtgccacagg gagccgtcct cctcctcgct ttcgacctcg
    20701 aggtcggaca cggccgactt cagcaccggg tcccagttga ccagccgctt gcctcggtag
    20761 atcaggccct cttcgaacag ttgaacaaac gtcgcggtca cgactttcga cagcttgtcg
    20821 tccatcgtga agtactcgtg cttccagctg acgctgtcgc ccatgcggcg catctgctgc
    20881 gtgatggtcg agcccgactg ctgcttccac tcccacacct tcgcgacgaa gttcgggcgc
    20941 ccgaggtcgt ggcggctctg cttctgttcc tgcaactggc gctcgacgac gatctgtgtc
    21001 gcgatgcccg cgtggtcggt gcccggcacc cacagcgtgt tgtgcccgcg catgcggtgg
    21061 tagcgcgtca gcgaatccat gatcgtctgg ttgaacgcat ggcccatgtg cagcgtgccg
    21121 gtgacgttgg gcggtggcag ctgaatcgag aaagagggct gggcggcatc gagcgttggc
    21181 tcgtagacgc cgctttgttc ccacagcggc ccccaatggg cctcgatggc ggcgggttcg
    21241 aaggacttgg ccagttcggt catggggaca ccgggttggg cgccgcgaaa tcgggcgccg
    21301 gagagcagaa aaacggaggc cgaattgtag gagggcggct cctagaatcc cttgatgtcc
    21361 agccccccgg aaagcccagc tgccgtcgct cctcacgagg atccctcgat acccctgttc
    21421 gaggaagacg agcccccggg tgccgcgggg cgcgccacgg gtgagatcga tgcggagccc
    21481 ggcctcgccg ttccttcgtc cgacaacctg ttgcgcgggc tgaatccgga gcaactggct
    21541 gccgtcacct tgccggcgcg cccgtcgctg atcctggccg gcgccggttc gggcaagacc
    21601 cgcgttctga cgacgcgcat cgcgtggctg atccagacgg ggcagctgtc gccgggcggc
    21661 gtgctggcgg tgaccttcac caacaaggcc gcgaaggaaa tgctgactcg gctgagtgcg
    21721 atgctgccgg tgccggtgcg cggcatgtgg gtcggtacct tccacggact gtgcaaccgc
    21781 ttcctgcgcg cgcactggaa gctggccggg ctgccgcagg gcttccagat cctcgattcg
    21841 gcggaccagc tgtcggccgt caagcgagtc atcaaggcga tgaagctcga cgaggagcgc
    21901 ttcgtgccga agaccaccac ctggttcatt gccgggcaga aggaggaagg cctgcgtccg
    21961 aaggacgtcg aggtgcgcga tgagcagagc cgcgtgctgg tgcaggttta ccaggcctac
    22021 gaggagcagt gccagcgcga aggcgtcgtc gactttgccg agctgatgct gcgcacctac
    22081 gagctgatgc gcgataatca gagcctgcgc gagcattacc agcgtcgctt ccggcacatc
    22141 ctggtcgacg aattccagga caccaaccgg ctgcagtacg cctggctgaa gatgtttgcc
    22201 ggcccgcccg gaccgaacgg caccgcggtg ctcgcggtcg gcgacgacga tcaaagcatc
    22261 tacgcgttcc ggggggccca ggtcggcaac atggccgact tcgagcgcga gttccgggtg
    22321 cagcaggtca tcaagctcga gcgcaactac cgaagctacg gccacatcct cgatgcggcc
    22381 aacgagctga tctcgcgcaa ctcgcggcgt ctgggcaaga acctgcgcac cgaggccggt
    22441 ccgggtgagc aggtgcgcgt attcgaggcc accagcgact tcgccgaggc gcagtggttc
    22501 atcgaggagg cgcagcagct gcaccgagac ggcgtggctc gccgcgatat cgcgctgctg
    22561 tatcgcagca atgcgcaaag ccgcgtgctg gagagcgcgc tgttcaacgc gggcattccg
    22621 tacaaggtct acggcggcct gcgcttcttc gagcgcgccg aggtcaagca cgcgctgagc
    22681 tacctgcggc tgatcgagaa cccgaatgac gacaccagct tcctgcgcgt cgtcaatttt
    22741 ccgacccgcg ggatcggcgc gcggaccatc gagcttctgc aggatgcggc gcgtgccagc
    22801 ggacgcagcc tgtaccagag cgtcggcgcg gttgccggca agggcggcgg gaacgtgcag
    22861 gccttcgtcg cgaaagtcga cgcgatgcgc gaggccacgc gcgggctgac gctgcgcgag
    22921 atcatcgagc acatgctgaa ggcgtcgggc ctggtcgatt tctaccgcac cgacaaggaa
    22981 ggtcaggatc gcctcgagaa cctggacgaa ctggtcaatg cggccgaggc gttcgtcacg
    23041 caggaagggt tcggcaaaga tgcggtggcg ctgccggtcg atgagttttc gaccccggtg
    23101 ccgatgggtg ccgattcgga gctcacgaaa gtgctgtccg ggctggggat cgaggcgggt
    23161 gctgccgcgg cgcaatccgt tggcggcatc gcgtcgctga tcaccacgcc cgatgccgaa
    23221 accggcgaga tcatgtcgcc gctcgccgcc ttcctgacgc acgcctcgct ggaggccggc
    23281 gacaaccagg cgcaggccgg ccaggacgcg atccagctga tgacggtgca tgccgccaag
    23341 ggactggagt tcgatgcggt gttcatcacc ggcctcgaag agggcctgtt cccgcacgag
    23401 aacagcgtgt ccgacctcga cgggctggaa gaagagcggc ggctgatgta cgtcgcgatc
    23461 acgcgggcgc gcaagcggct ctatctgagc tgcagccaga cccgcatgct gcacgggcag
    23521 actcgctaca acatcaagag ccgattcttc gacgagctgc ccgaggcttc gctgaagtgg
    23581 atcacgccgc gcaaccaggc cttcggctcg ggcttcacgc gggattacca ggccgcgtgg
    23641 gcacgcgggt cgggactggg atcgatcgtc ggcgccgggc gcgtcgcgac ggcgccgcca
    23701 tcggtgatcc cgaaggcgcc atcacacggc ctgcgatcgg gccagagcgt gttccacacc
    23761 aagttcggcg agggcgtgat cgtcacgctc gaaggcagcg gtgacgatgc ccgtgcccag
    23821 gtcaacttcg gccgccacgg catgaagtgg ctggcgctgt cggtcgcgaa gctgacgccg
    23881 attccttgac tcgacagtct cagtcgtttc gcttcctccg attcaagctg cccgcacgtt
    23941 tacagcgttt catgcgcttg ccgaggccat cgggcggccg gctccgctca tgtcccccgt
    24001 gacgggctgc gcccgcccct cctcctttac ttcgctgcgc cagccacccg atggcctcgg
    24061 cacgagtcgc tgttgtttgg gtgctcgaac acccgcagcg atggctgaga agacggtcgg
    24121 ggtgttctgt gtagcgaggt taaggaggag acccggccac aggccgggtc gggggacacg
    24181 agcgaaacag agtaccccga ccgtcggctt acgcgccgat cccaataaca agaggcccca
    24241 actgtattga ctagaccgat gccgcgtcga tctgctggta cgcgtcgcga agccacatct
    24301 tcagccgctg gcggtccatc atgccgagtc cggtgcgtga cttgtcggcg tcatgctgcg
    24361 ctgcccagaa gctgctgctc gacgcgtcga tcttctggat gaccgcgtcg ctcaggtgct
    24421 tgatggacac cacgcgggca ccaaagccga gcgcgcgatc ctgctcgccc gactgctcca
    24481 gcatggcgaa gtcgtcgccg cgcatctggt tgcgaaccag cacgtagttg agtcgcgcgc
    24541 cgaagcggtc gagcagcttc ttcagcagat cgaccgagtc acggcccgaa tccatcacat
    24601 gccagtaggt gatcgtgaag ccggcttcgg cagccatgtc gagcacgccc gattcgtcca
    24661 tccacttgac cagcggatcg tgcgtctgcg cggccagatc gaccaggatg cggcggtcgg
    24721 gttgctcgac cgcagcctcg atgatcgagt cgagcgcctc gtagcggtcg atcagcaccg
    24781 gcgacgcgaa gccggcatag aagcgcagca acgcgccgtg cgagcggtcg gtgtcgaagc
    24841 cgaggaacgg caactccttg tcgatcaggt actgcgcgac gaggcgcgac acgagcgact
    24901 tgcccacgcc gcctttctcg ccgccgatga agtggatgtt ggccatgtgg ggactttctt
    24961 cgcagggtga tgaggatggg tcgagggctc tgcggcccgg tggtggacac ccgcaatcgg
    25021 catgccatcg gttggttctg ccgcgcgctt gccctgcgtg caacgaaaac tgcctccggt
    25081 cgggcgtccg accggaggca gtgtcgcagg ctgggcacga tgaccgggcg tgaacccggc
    25141 cgaaatgcgg atcaggtgcc caggcgctcc tcgatgcgcg ccatcgtcgc aggcagctgg
    25201 ctcggcagat ggaattccag ttgcttgaac agctcggcgt gcaagaccag ttcctgcttc
    25261 caggcggcct tgtcgatccc gacgacgcta gcgaactgct cgcgcgtgaa gttcaggcca
    25321 tcccagtgca ggtcgtcgta gcgcgggctg acgccgaaca cgttgtcttc gccaccggct
    25381 gtgccatcca cgcggccgag catccactcg agcgcacgca tgttctcgcc gtagcccggc
    25441 cagacgaact tgccgtcggc gcccttgcgg aaccagttga cacagaagat ctgcggcagc
    25501 ttggcgccgg tggcggccag cttttcaccg aggttcagcc agtgctggaa gtgatcggcc
    25561 atgttgtagc cggcgaatgg cagcatcgcg aacggatctc ggcgcaccac gccctgctgg
    25621 ccggctgctg cggcggtggt ctcgctgccc atcgtggcgg ccatgtagac gccctcgacc
    25681 caggtgcgag cctgtgtcac gagcggcacg gtcgtcgagc ggcggccgcc gaagatgaac
    25741 gcatcgatcg cgacgccggc ggcgttgtcc cactcggggt cgagcaccgg gttgttgccc
    25801 gcggccacgg tgaagcgcgc attcgggtgc gcggccttgg cgccggtttc cttggcgatc
    25861 gcaggcgtcc agtccttgcc ctgccagtcg atcaggtgcg ccgggggggt gtcggtcatg
    25921 ccttcccacc agacgtcgcc gtcgtcggtc agcgcgacgt tggtgaacac cacgtcggac
    25981 ttcagcgaat ccatgcagtt cggatttgtc ttgtggttgg tgccgggcgc cacgccgaaa
    26041 tagccggctt ccgggttgat cgcgtaaagg cgaccgtcgg tgcccggctt gatccaggcg
    26101 atgtcgtcgc cgatcgtggt gaccttccag ccgacatagc cgtccggcgg caccatcatc
    26161 gagaaattgg tcttgccgca ggcgctcggg aaggccgctg cgacgtggta cttcttgccc
    26221 gccggattgg tcacgcccag gatcagcatg tgctcggcca gccagccctg gtcgcggccc
    26281 atcgtcgacg cgatgcgcag cgcgaagcac ttcttgccca gcagcgcatt gccgccgtaa
    26341 cccgagccgt agctccagat ctcgcgcgtt tcggggtagt ggacgatgta cttcgtgctg
    26401 ttgcacggcc aggccacgtc cttctggcca gcttccagcg gcgcaccgac ggtgtgcacg
    26461 cacgggacga aatcgccgtc gctgcccagc aggtcgatca ccggacggcc catgcgcgtc
    26521 atcgtgcgca tgctcaccgc cacgtagggg ctgtcggtca gctcgacgcc gatgtgcgag
    26581 atgtgcgagc cgagcgggcc catcgagaac ggcacgacgt acatcgtgcg gcccttcatc
    26641 gcgccgcgga acagtgcctt gtcgccggat tgcagcaggg tgcgcatctc ggacggctgc
    26701 atccagttgt tcgtcgggcc ggcgtcttcc ttgcgctggc tgcagatata ggtgcggtcc
    26761 tcgacgcggg ccacatcgct cgggtcgctg gtggccagga agctgcgggg acgcagctcg
    26821 gggttgagct tcttcatcgt gcctgcggcg accagctggt tgcacagtcg gtcgtattcg
    26881 gcttgcgtgc catcgcacca atagacaccg gatgcgtcgg tcagggtcgc gatctcgtcc
    26941 acccaggcct tcaggcgctg gtgcttcatg tcggcgggca ggttggtgtg ctgcctttcc
    27001 gtggcgttgc ggttcatggg atctccaatg aaagtaaaga aaaaacggaa tttggcgagc
    27061 cccgcctggt gccgcgaggc tcgccgaatg ccacgatttc gtccccacct gtcgatccgg
    27121 acggcggggg ggctaattgt gggggcgaat cgacccgtcc gttcaccacc cgcccgcagg
    27181 ggtccggtga ctgaaacgag cttcccgtgg catccgccac gccttcccgg cgtgttcggc
    27241 ggtcgttcag cccgccagcg cgcccggacg accggcttcc accgcgaatt ccgcgccggt
    27301 gcgcaccggg ctggccgggt ccttgacgtt ccccacgacc cgcagcgcga cctgcaggcg
    27361 attcgcgtcg agcgtgaaca gcatgctgcc gcgctggtcg ccgcggctga ggtggatgtg
    27421 agggttgaac ggtcgcgccg cgtcgatcct gtcctgtggc atgccctggc tggagatcga
    27481 ggtgccgcag aactcggtgg cgacggtggg cccctcgggg cggtcgaaat cggccttcag
    27541 atcggccacc acatgggcgt gcacgtcgcc gcccagcacc acgaccccgg gtacctggcg
    27601 ctcggccacc gtgccgagca gccggttgcg tgacgcgaca tagccgtccc agccgtcagt
    27661 ccagtaggtt ccgccgctgg ctggatcgct ccagctgtgg cgtgccatca gggtctgctg
    27721 cccgaccagg ttccacgacc gctccagatc ccagccggtg gcgagccagc gctcctggtc
    27781 gagtcccagc aggctgcgtt gcggatcgcg cagcgcaggg cagtccttca gcgccaccgt
    27841 gttcgagccg ccctgtccgg gtcgcgggca ggcctggggt gcgcggtgct gccggtcgtc
    27901 gagcaggtgg atgcgtgcca gcgcgcccca gtccaggcgg ccggtgatgc gcatcgccgc
    27961 accggttgcg tccagccggg gccgggcgga ggagggaaac gggagatgct cccagcaggc
    28021 ctggtacgcg gctgcccggc gggatgcgaa atcggtgtcg aggtgggccc cctgcaaccc
    28081 cgcgtagtcg ttctcgacct cgtggtcgtc ccagatcgtc agccacggag cactggcgtg
    28141 agcagcttgc agcagcggat cacgcttgta ggtggcgtag cgggcccggt actcctccag
    28201 cgttcgggtg acaccgcctt cgtgacgtcg caggccgtcg ggccgcgagc ccgactcgta
    28261 gatgtagtcg ccgagaaaca gcaccagatc caggttctgg gctgcgatgt cgcgccaggc
    28321 tgcgtagtgc cctgcgtcgt agcgctggca actggcgatg gcgaagcgca gcggggctgc
    28381 cgccccggat cgggcatcgg tggatggcgc ggtgcgggtg cgaccgatcg cgctgcggtc
    28441 acccagcgcc tgaaatcggt accagtacca gcgccccggt tccagcccgc gcggctcggc
    28501 gtggacactg tgcgcccagg ccgcttcggc ggtctcgatg ccgcgggcgg cgatgcgctg
    28561 gaacgcctcg tcatgcgcca gttcccaccg cacgtcgacc cggtcgggca ggccagggcc
    28621 ggtgagccgg gtccacagga ccatgccgat gctgctcggg tgaccggagg cgacgccgag
    28681 gtcgaagcgc gccacgtcag ctggcgcgga gcccggcgtc tccaggcgca acgcgggagc
    28741 gcagccgccc agcagccacg cgggggcgct tgcgcccgct gccgcggcga tgccggtgcg
    28801 gttcaggaac cggcgacggg tgcagttcat ggtcggtcct tgtctggcag ggcgtggctt
    28861 ggccccggat ccaccggtgc ttcagctgcc tatcgtgcgg ccgtcgccgc gggtgatcgc
    28921 gatcgtggcc gaccgggggc gaccgcccat cgccccgtcc ggccacgacg agtaccgcgt
    28981 tggcgacacc gacagatcgg cgtccgatcc cgcctcgccg ggatgctgga tgttgacgaa
    29041 catcgtcttg cggtccggcg tcatgtgggc gccggtgacc tcgcacttca ccggcccggt
    29101 caggaaccgc cggatgcggc cggtggacgg atcggccatc agcatctggt tgttcccgaa
    29161 ttggccgaac tccgggtcct gactgtccgg gccgccggtg ggcaggcgcg ccggtgacac
    29221 gtcggtctgg atccacagca tgcccgtgtc gtcgacccac aggccgtccg ggcagccgaa
    29281 caagtcacca tcgatcgagc cctgtcgtgc cggatcggcc tgggctgggt cgcccgcgag
    29341 cacgaagaca ttccagcgga aggtgaggct cgcggggtcg ccgccgtcgt ccttccagcg
    29401 cacgatgtgg ccgtagatgt tgctggcgcg cgggttgacg cggtccgtcg gggggcgcgc
    29461 cgagctgccc gcgctgcttc catcgacggc gtgggtggaa gccggcagcg tgccgcgccg
    29521 tgtgttgttg gtcagcgtgg cgaacacctc tccggtgcgg cggttgacgg ccacccattc
    29581 cggccgatcc atcagggtgg cgcctgcgcg gtcagcggcc tgtcgggttc gaatcgcgat
    29641 gtcggccggg ctgctccagc cgtgctcggc gctcaggccg ctgcgccccg gtgtcagttc
    29701 gagccagacc ccgctgccgt cgtcatggaa tttcgccacg tgcagcgtgc cgtggtcaag
    29761 cagatcccgg ttggcgtcac gccgcttcgg atcgaacttt ccgtcggtga cgaacttgta
    29821 gatgtactcg ttggcctcgt cgcaacccat gtagaacgcc agccggccgt ccttggtcgc
    29881 cagccactcg cagttctcgt gcttgaaccg gcccatcgcg gtgcgcttga ctggacgcct
    29941 gccgggctcg taggggtcga tctccacgat gtagccgaag cggttcgcct cgttcggttc
    30001 gcgagccagg tcgaagcgcg gatcctgctt ccaccagggg tacaacgggc cggcatggcc
    30061 gatggcgccg cggaccgaca ggccatagcg ccgctggtcg gcggtgggac ggaacccgac
    30121 gtcgtcgctg ccgaacacgg cgttgaagtt ttcctcgcag gtcaggtagg ttccccaggg
    30181 ggtgaacccc gccgcgcaat tgccgagcgt gccgcgtacc cgcgtgccct gcggatcggc
    30241 cgcggtcttc agacgcgggt cgccgcgggc ggggcccgcg atgtcgatgt cggtgtcggc
    30301 ggtgatgcgg cgtgcgtggc gggagttcct gacgacggcc cagcggccgc tgcgatcgcg
    30361 ggcgaactcg accaccgaaa cgccatgcgc cgccttcgat ttggcgacgc cctccgggct
    30421 gctccaccgt tcgggcggcc cgatcagcag gctcggatcg acatactcgt gattgacagc
    30481 ccatagtccc gatgttgacg agcctgcctt ccagtctggc aagctgaaga aatgcccgcc
    30541 gtcgtggtgc atgccggcct gccgggcctg atcggctgcg ctgttgctgc catcgggcat
    30601 gaaggcggga ccgacgccat cgatcggatc cccccaggca taaacgacct gaacccggta
    30661 cccggccggc acgcgcaccg tgtcggcctt cgatgcctcg acgggatcga agaaagcctt
    30721 cggtgcggcc ggcccgctcg gtgttcggga atgcgcggct gcgggcagca ccggccccag
    30781 gagcgccgcc agcgctgcgc ccgcgccccg gcggagcaac tcgcgccggc cgggtgatcg
    30841 ctcgacgatg tccgcgaagc ggtcctgagc tgtccggtcg cgccgtgcgg tgcaaggtgg
    30901 ttgattcaat cgtgctcccg gatggttggg gtgcgcggag cctgcttgcc ccgtgggttt
    30961 catgcaccca tgcgaaagca gtatccatcg ggccaacgga agccggcggg tgcgcattgc
    31021 ggacgcgggg cctttcttca tgctgtcaca cgcgaccgtg atagtccgcg gcagccgtgg
    31081 ctccatgccc ggtgagggtg cgccgcgacg gcgtgccgcg gctctcgaat atccccgaac
    31141 aaccacctcc aggagtaccc gatgaagggc aaatcgcacc ccaccaacga tgtgtcgctg
    31201 aatccgtcga gcggtcattc gatccgcgac gtgatcgatc acgtcgatgc aagccgccgt
    31261 cagttcatcc agagcggcgt tggcgccgct gcgctggcat cggccggcgg catcacgctg
    31321 ggcggactgg tcggcacggt gcaggccgcg ccggttccgg ccggtctggg cttccccggc
    31381 atcggcttcg agagcattcc cgccagcatt gacatcgatc cgagcagcac cgcatccgcc
    31441 gtcgccgacc tggtcaaggt gcccgttggt tacaccgcga acgtgttcgt tgcctggggc
    31501 gacccgatca tgccgggtgg cgcgcctttc agcggcacgg ccagcgaaac cgctgccgac
    31561 cagctcaagc agttcggcat gcacaacgac ggcatgcact tcttcccgat cgacgacgac
    31621 cgcgacgacg acaagcgccg tgacgggcac gacggccatg acgacgnnnn nnnnnnnnnn
    31681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnngacg acgaccgcga cgacgacaag cgccgtgacg
    31801 ggcacgacgg ccatgacgac gaccgcggct ccagcaagcg cggcatcctg tgcgtgaaca
    31861 acgagtacac ccacgaggaa gtgctgttca ccgacgggca ggtgggtgcc ggctattcaa
    31921 tcgccaagac gcgcaagtcg caggcggctc acggtgtctc gatcgtcgag gcgcgccgca
    31981 agggcggcaa gtggtcggtt gcgaagaact cgtcgctcgg tcgccgcatc actgccaaca
    32041 ccttcatgca ggtgtccggc ccggctgccg gccacgcgct gatgcagacg cgtgaattcc
    32101 tgatcaccga tgtcgccagc acgccgaccg gcaacaccac gaacggccgc gtggccaatg
    32161 gcacggtgaa caactgcgct cacggcatca cgccttgggg aacgtacctg acctgcgaag
    32221 agaactggaa cggcaacttc ggtggcaccg gggcggtcga cacgtctgtc acgaccgaga
    32281 tcggcaagct gaatcgccgc tacgggctca acgcaggcgg cttcggctac cgctggcaca
    32341 ccaccgaccc gcgctgggac ctcaccacca acccgaacga agcgcacctg ttcggctggg
    32401 tgatcgagat cgatccgtac gatccgaagt cgattcctgt caagcgcacg gccctgggtc
    32461 gcttcaagca cgagagcgcg cagtacgtcg tcgacaagga cgatcacgtc gccttctact
    32521 tgggcgacga cgagcgcaac gagtacatct acaagttcgt ctgcaagggc aagctcaaga
    32581 aaggccgccg cgccgcgaat cgcaacctgc tcgacgaagg cacgctttac gtcgcccgct
    32641 tcgatgccga cctgaccggc gcgtggctgc cactgacgcc ggcctcgatc gggctcgatg
    32701 gcaaggccct gcgcgagaac gccaacttca ccggcgccga tgatgcggaa gtcctggcca
    32761 agatcctgat caagacccgc atggccgccg acgcggtggg cgcgacgatg atggaccgcc
    32821 ccgagtggac tggcgctcgc ccgcgcatca atggcttcaa ggaggtcgag gtctactgca
    32881 cgctgaccaa caacaaccgc cgcggaaccg ccggtgcatc caccaatgcc gccgatggtt
    32941 cgacgtcagc gggttcggct cgccctccag tggatgccgc caacccgcgt gaagacaacg
    33001 tgcacggcca catcatccgc tggcgcgagg ccggcaagtc ggtgaaggcc accgcattca
    33061 cctgggacct gttcgcccag gccggcgaca cgctgaccgc caaggtcgcc aagccgacga
    33121 acaactatca gggcaacatc gtcgacgccc cgaacggcag cgctgattac ggcgcaccgg
    33181 acggcctgtg gttcgactac ttcggccgcc tgtgggtgca gaccgaccag atcggcaacg
    33241 gatccggcga cttcgtcaac atcggatcga acgtgatggt gtgcgccgac ccgaacaccg
    33301 gcgaaacgcg ccgcttcctg acctcgccga acaaatgtga agtcaccggc gtgacgatga
    33361 cgccgaacgg ccgcacgatg ttcgtcggca tccagcaccc gggcgaggac gccctggcag
    33421 cgaatccgac ggagttctcg aactggcctc agggtcagtt cgctgtggag tccgacggcg
    33481 tcaccgcgtt gccgggtggc cgtccgcgct catcggtggt cgtgatcacc aaggacgacg
    33541 gcggcatcat cgggacctga ccgaccgatc cgctcgaccg ggccccccgg actgcatcga
    33601 tcgaacgatg cggcccgggg ggccttcttt cttggcgcga cgatgaaacg cgggaatgtc
    33661 agccgcgtcc gatgtacggc atggccgttg ccatgatcgt catgaactgc acgttggccg
    33721 acagaggcag gctggccatg tgaatcaagg cgttggcgac gtgcgccacg tccatgcgcg
    33781 gctcggccat ccggctgccg ttggcctgca ggatgccggc tgccatgggc gcggtcatgt
    33841 cggtggctgc gttgccgatg tcgatctgtc cgcaggcgat gtcgtgttcg cggccttcga
    33901 gcgacgtcga gcgtgtcagt ccggtgattg cgtgcttggt cgcggtgtag ggcgtcgtgt
    33961 agggacgtgg cacgtgggcc gagatcgacc cgttgttgat gatgcgaccg ccgcgcggcg
    34021 tctgggcctt catgaggcgg aatgcgtgct gcgtgcacag gaaggcgccg gtcaggttca
    34081 cgtcgacaac ggtcttccat tgctcgaagc tcaactcgtc gagcggcacg gccggagcac
    34141 cggtgccggc gttattgaag agcaggtcca gcctgccgca gatgcttcgt acacgctcga
    34201 aaagggcggc caccgaggcg ggatcgctga cgtcggtggg cacggcgagc gcatgggtcg
    34261 cgttgacacc cgcggcattc gcggtcgcgt ccaggctcgt cttgttccga ccggccagca
    34321 cgacgatgta gccggcttcc agcaaggcca gcgagcaggc ccggccgatt ccgcttccgg
    34381 cgccggtcac caccgcaaca ggcattttct cgatcatggt gatggctcgg cggcgggcgg
    34441 tgcaagggcc gcggtgcgtt gttcgaaaca gtccgtgaag gtgaagtaga gcgatgcgta
    34501 gaacaccgtg gagaacatca ggccggcggg cagcgctgcc acggcgacca gctgcggtgc
    34561 acccagcagc atgaacaccg tgttggcaag cagcgcgaac aggaacacca gcgcagccca
    34621 ggtcaggccg tacaccatga acgcgccctt gttgcgccag caggcgatgg tgctcgagaa
    34681 cagcgactgc gccgggccct ggccgtccca gtgcaccagc gccggggcat gccagaaggg
    34741 cagcgcgagc aggctggcca ggcccaggcg caccagcatg ccggccgaca ggcgcgggtc
    34801 gttcagcaag acgtcgaggc gttcggtgtc ggcggtcttc gcggtcatgg cttcctgcag
    34861 tgcatcgaag gtgcccccgt ccaccacgtt gctgaaccac atgatgaaca ggctggcagc
    34921 cgagtagatc aagccgatct tcagcatggc gatcgcctgc ggacggcgtc cacgcaccgg
    34981 ctcgatgaac acgccggcca tcggcgtctg tccggccagt gcgcgttgag tggccagcat
    35041 gaagccttgc gagaccagcg gcagggcagt cagcagcagc agcgtaccga tcacgggcac
    35101 cagcaacagc agcatcgcac cgaacaggaa gctggcgaac agcgcggcaa acgccagcgg
    35161 ccggccgaag aagacgcgaa atccgctgcg tacccagaga acgccgcggc ttgccggcac
    35221 ggtcaacagt ctcattcagc gtccggcgtg gcgtctcgtg atagggggag gggcggatgc
    35281 cacggggtgt cggcccgctg tcgcagcacg cgttcgaagt gggtcggatc atgcgcggtc
    35341 agcatggcgg cgtcgcgcgg caggtgcaga tcccacaggc gcgagatcca gaacctcagg
    35401 gcacctgcac gcagcatcgc ggggaacagg cgcacctcgt ccccggtgat cgggcggacc
    35461 gcatcatagg cagcgaccag tgccaaggca cgatcctcgt cgaggcggcc ggtgaccagg
    35521 tcgatgcacc agtcgttgac gcacacggcg atgtcgaaca ggaacgtgtc gacgccggca
    35581 aagtagaagt cgaagaagcc gctcagccgg tcgtcgccct cggcgctctc gtcgaacatc
    35641 acgttgtcgc gaaacaggtc ggcatgaacc ggtccccgcg gcaggcgctt gcaggcggct
    35701 gactgcgcca gatgctgctg gaaggccagt tccgcctcga tcagcgtgcc ttgcgattcc
    35761 gtgaggaagg gtcgcaccac aggtaccgtc tcggcccacc aggcgaggcc ccgcaggttg
    35821 ggctggtgca actcgaaatc ggccccggcg aggtgcatgc gcgccagcat cgcgccgagc
    35881 tgcctgcaat gctcgacgtc gggcgccagc cggctgctgc cccgcaggcg atcgaccacc
    35941 gcggccggct tgccctgcag tgtgtgcagg atctcgcccc cggcgtcggc gcaaggatcg
    36001 ggcaccggga tccctcgctg tgccagatgc ttcatcagcc gcaggtagaa cggcagttgc
    36061 tcggcggtca gacgctcgaa cagcgtcagg acgtagcggc cctgcgtggt gtcggcgaag
    36121 tagttggtgt tctcgatgcc gcctgagcag ccttcgagcg tcgtcaggcg gccgatgttc
    36181 aagcgcgaaa cgaattcagc cgccgtatcg aacggcactt cggtgtagac agccatcgca
    36241 gacagacgcg aacgagagtc gcgggaaaga agttggagtg agggtttctt gaccgccggg
    36301 gcggccgcgg tcagaaggac agcacgggcc acacccgttt acctaccgaa ccgttggagc
    36361 cgttcggacc ctcggtctgg tcgggtgcgc cgctgggcgg gatgatttcg tagggcttgc
    36421 cgcccgtctt cggggtgacg gtgatgcgct tggtctggcc gcgcactttc agttcgtcga
    36481 tgcgggcgcc atcgtcctcg atgactgtcc tgcgcaccgc aggttctccc tgcgcggtct
    36541 tctcctctgc ggcggtgctg gctggtgcca gcggtcgggc cgacggctgg acagccgacg
    36601 ccgacgggcg tgccgcagcc ggaacccgtt cctgggccga agcgaccaga tgcaagctgg
    36661 ccgcgcagca cagcgcgagg agtgcgggga gccggcggtg tgatgtgagc atcgccgcat
    36721 tttaggcgcc gcggcatttc cggcggcatg catggccgct ggtgcagctg cgccgcagcg
    36781 aagcgcccca ccgttcaggt gggccgcaca attcgtcgat gagttccacc ccgctccccg
    36841 tgatgatgct ggtcgacgga tccagctacc tctaccgcgc ctaccacgcg ctgcccgacc
    36901 tgcgtggccc ggacggattc ccgaccggcg ccctccacgg catggtggcc atgctgcagc
    36961 gtgccctgaa ggatgtgaat cccgagcatg cggcgtgcgt gttcgacgcg aagggaccga
    37021 cattccgcga cgactggtat cccgagtaca aggctcatcg cagtccgatg cccgagccgc
    37081 tggtgcagca gatcgagccg atccacgagg tggttcggct gctgggttgg ccggtgctgg
    37141 tggtgcccgg catcgaagcc gacgacgcga tcggcacgct ggcgcgggtg gcggccgcgt
    37201 cgggccacca ggtggtcatc tccacgggag acaaggatct ggcccagttg gtcaacgagc
    37261 gcgtcacgct gatcaacacc atgagcaacg aacggctcga cgtgcagggg gtcatgacca
    37321 agttcggcgt gccgccggaa cgcatcgtcg attacctgac gctgatgggg gacacggtgg
    37381 acaacgtgcc gggcgtcgac aaggtcggcc ccaagaccgc ggcgaaatgg attgccgagc
    37441 acgggtcgct gcagggcgtg atcgacgcgg cggccagcat caagggcgcc gccggggaga
    37501 acctgcgccg cgcgctcgac tggctgccgc aggcccagcg gctggtcacc gtgcgcctgg
    37561 actgcgacct cgccggtcac gtgccgggtt ggccggggct cgatgcgctg gccttgaaag
    37621 aggccgacaa ggtggctctg cgtgatttct acgcgcgcta cggattcaag acgtggctgc
    37681 gcgacatcga gtcgcagctc ggggtcgccg cggccgagcc tccggctcgc aagcgctcgg
    37741 tggcggcgcg ccccgcgtcg ggcgaaccgg acctgttcga gacagaggtg gaaaacaaca
    37801 gtgaggttgc cggcgccgct gcgcctgccg acccggtcga gcatcgctac gacaccgtgt
    37861 tgacgatcga agcgctcgat gcctggatcg cgaagatcga ggccgcttca cgaagcggtg
    37921 ccgtcaccgc gatcgacacc gagaccgatt cgctcgatgc catgaaggct cgactggtcg
    37981 gcatctccct gagcgtggcg cccggcgagg cggcctacat cccgctgcgc catgactaca
    38041 ccggcgcccc cgagcaactg tctccggtcg atgtgctggc gcggttgaag ccctggctcg
    38101 aagacgaagg tgcacccaag ctgggccaga acatcaagta cgacacccat gtgctgcaga
    38161 acgccggcat ccaagtccgc gggtacgtgc acgacacgat gctcgaaagc tacgtgctcg
    38221 aggcgcacaa gccgcacggt ctggaaagcc tggcgcagcg tcacctcggc cgcagcgggc
    38281 tgagctacga ggatctctgc ggcaagggcg cgcaccagat cccgttcagc caggtcgatg
    38341 tggcgaaggc ggcggtgtat tcgtgcgagg acagcgacat gaccctcgac gtgcatcgca
    38401 cgctgtggcc cctgatcgac gccgagcccg gactgcgcga cgtctacgcg cgcatcgagc
    38461 tgccgaccgc gctgctgctg cagcgcatcg agcgcaccgg cgtgctcatc gacccggccc
    38521 gactcgcttc gcagagccag cagctcggcg aacggatggt cgctctggag tccgaggcct
    38581 acgaactggc cgggcagccg ttcaacatgg gcagcccgaa gcagatcggc gagatcctgt
    38641 tcgtcaagct gggcctgcct gtcaagaaga agaccgccac gggtgcaccg agcaccgacg
    38701 aggaagtact ggccgaactc gctgccgact acccgctgcc ggcgcgcctg ctcgagcacc
    38761 gcagcctgtc gaagctcaag ggcacctaca ccgacaagct gccgctgatg atcaatcccg
    38821 acaccggtcg cgtgcacacc aattacgcgc aggcggtggc ggtgaccggt cggctcgcca
    38881 gcaacgaacc caacctgcag aacatcccga tccgcaccgc cgagggccgc aaggtgcgcg
    38941 aggccttcat cgcgccatcg gggcatgtca tcctgagtgc cgactactct cagatcgagc
    39001 tgcgcatcat ggcgcacatc agcgaggacc cgggcctgct gaaggccttc gccgagggga
    39061 tggacgtgca tcgggccacc gcgagcgagg tgttcggcat cgagcccgcg tctgttacct
    39121 ccgaacagcg gcggtatgcc aagaccatca acttcgggct catctacggc atgggcgcgt
    39181 tcggcctggc gcaaagcctg ggcatcgagc gaagcgccgc tgcggcctac atcgaccgtt
    39241 acttccagcg cttcgccggg gtcaagcgct acatggacga cacacgggag tcggcacgcg
    39301 aaaagggcta tgtggaaacc ctcttcggtc gccgcgtgta cctgcccgag atcaacggcg
    39361 gcaacggacc gcgcaaggca ggcgccgaac gtcaggcgat caacgcgccg atgcagggga
    39421 ctgccgccga cctgatcaag ctggcgatgc tggcggttca gcgcgtgctg gacgccgagg
    39481 gtcgcgcggc gcgcatcgtg atgcaggtgc acgacgaact ggtgctggaa gttccggatg
    39541 ccgagctcga ctgggcgcgg gcagcggtgc cggcagcgat gggttcggtg gcctcgttga
    39601 aggtgccgct gctggcagaa gtgggtgtcg gagccaactg ggatgaagcg cattgatgcg
    39661 gtacccgcct tgctcggcaa ggcgctggtc cgcgatccgg cgcagcgctt tcccggtggc
    39721 taaggttcgg attgcctgcc ccgagccagg atgtagatat cccacacggc catgaacatc
    39781 gctgcgatca caggcccgat gacaaagcca ttcagcccga aagcggccat gccgcccagt
    39841 gtggagatca gcaccacgta atcgggcatc ttcgtgtcct tgccgaccag gatggggcgc
    39901 agcacgttgt cgaccagacc gatcaccaga acgccgtagg cgatcagcgc aaggcccggt
    39961 ccgaccgcgc cggtgaccag gaagtacagg gccacgggca gccagatcag tccggccccg
    40021 atcgccggca gcagcgacag gaaggccatc agcaccgccc agaacagcgc accggtgacc
    40081 ccgaggaacc agaaagccag gcccccgagc gctccctggg tcgccgcgac gaccacgttg
    40141 cccttgaccg tggcgcgtat caccgtcgtg aactttcgca gcaggtcgcg cttgtcctgc
    40201 tcgtcgaggg gtatggccgc gttgatccgc agcgccaggg cagctccgtc gcgcagcagg
    40261 aagaacacca gatagatggt gatggaaaag ctgacgatga attcgaaggt gatctggccg
    40321 atgttcagcg cctgggtggc gatcaggctg gtgccctgcg tgatcgccgc ggtgagcttc
    40381 tgcttcaggg cggcgatgtt cccgaagccg aactggttca tcaagtcctt gatccaggac
    40441 ggcagagcga gaacgatctg ctcgaagtag cggccgaaat ccagttcgcc ggactgtgtg
    40501 cgtgcataga cgctggcgcc ctcgcgggcc agcgaggcag cgatcaccgc cagagggatc
    40561 agcacgatca gcacgatcgc agccagcgtg atcagcgctg cgagcgaggg ccggtcgggc
    40621 agcctgttgc gcagctttcg atgcagcggc gtgaacagga tcgccagcac cacgccccag
    40681 aacaccgacc cgaagaacgg cgtgaggatc cacgcgaacc ccagcgacac cgccaccagc
    40741 acgatcagga aaaccttgtt ttcgagcacg gagtcgcggt tcggatcggg tggtgacatt
    40801 gcggcggcag gtgcggggcg gtggcgcatg gtatcgcccg gtctgtcacg gtcctgtcat
    40861 ggatctgaca catgattgag ggcgacgcgc cgacgtgtgc accatctgcg caggatccgt
    40921 ctgggcgggc catccttcat aaactagccg aatgagtacc gaacccgcgc agccagcgaa
    40981 tgacagcccc gtggccctga cagcgccggt cgataccgcc gccgttcctg ccactgccgc
    41041 acccgcggcc gtcccgcagc ccggggatcc ggcgttgatg ctcgggcggg aaacgtcgat
    41101 cctcgaattc aaccggcgcg tgctggcgca ggcactgcgc gccgatgtgc ctctgctcga
    41161 acggctgcgc tacatctgca tcgtgtcgtc gaacatcgac gagttcttcg aggtccggtt
    41221 tgccgattac ctcgaggcct cccggctgcc gaatgccgga gtcaccgatg ccgacgtcca
    41281 ggcgatcacc gccgaagcac atcgcctgat cgacgatcag tacgccgcat tcaacgatca
    41341 ggtgatgccg ctgctccagc gcgcgggcat cgtgatcctc aatcacgccg atcgggacga
    41401 ggcgcagcgc gattgggtgg acaacttctt ccagaagcag gtgcggccgc tgctggtgcc
    41461 gatcggactg gatccgtcgc atccgtttcc gctggtcgcc aacaaggcgc tgaacttcat
    41521 cgtgcggctg ggtggcaagg atgcgttcgg ccgcgagaac acgatcgcca tcgtgaaggt
    41581 gccgcgcgtg ctgccgcgcg tgatccgcct tcccgacgcg ctggtcggag gaaagcaggg
    41641 cttcgtcctg ctgaccagcg tgatccgtgc gcacctgggt gaactgttcc cggggcgcga
    41701 agtcgagacg ttctcgcagt ttcgtgtcac ccgcgattcg gacctcgaag tcgacgagga
    41761 cgatgtcaag aacctgaggc aggcactgcg gtcgggtctg agttctcgtc atttcggtca
    41821 ggcgatccgc ctcgaggtgg tgagcagctg tccgcccgag ttgtccgagt tcctgctcga
    41881 acagttcgac ctgccggagg cggcgctcta caaggtcaat ggcccggtca acctggtgcg
    41941 tctgaaccag ctgatcgacc aagtgaccga tcgcagcgcg cggccgccgc tgcgctttcc
    42001 gccgcatgag ggcgtctggc cggaacaacg tctgccgcgc ggccagtcga tcttcgccag
    42061 cctgcgccgc ggcgatgtgc tgttgcacca tccattcgaa tcgttcgatc cggtggtgca
    42121 gttcctgcgc gaagccgtca acgacccggc ggtgctcgcg atcaagcaga ccatctaccg
    42181 caccggcagc gagtccccgt tgatggacct gctgatcgaa gcggcgcgcc gcggcaagga
    42241 ggtgatggtc gtcgtcgagc tgaaggcgcg cttcgatgaa gaggccaaca tcaactgggc
    42301 tgaacggctc gaagccgtcg gcgcgcaggt ggtttacggc gtcgtcggtc tgaagacgca
    42361 cgccaagctg ctgctggtga cgcggcgcga ggtcggcacc gggggcgccg tgagactgcg
    42421 ccgctatgtc catctctcca ccgggaacta caacccgaag acggctcggc tgtacaccga
    42481 tgtcggctac ctgaccgccg atgccgagct gaccgccgat gccgatgcgg tcttccagca
    42541 gcttgccagc ctgggcaaga tgaagccgct gcggcagctg ctgcaggcgc cgttcacgct
    42601 tcatcgcagg ctgatccaca cgatcgatcg cgtggccgat gcggcgcggg ccggcctgcc
    42661 ggctcgcatc gtcgtgaagc tcaatgcgct gaccgaccca ccgctgatcg acgcactggt
    42721 gcgtgctggc caggcggggg ccgacatcga cctgatcgtg cgcggagcct gcgtgctgcg
    42781 ccctggcgtg gccggtgcca ccgatcgcat ccgggtgcga tcggtcgtcg gccgttttct
    42841 cgagcactcg cgggtcatgt atttccgctg gggaccgggc gacgatgacg aggtgctgta
    42901 cctgtcgagc gccgactgga tgagccgcaa catgcagcgc cgcatcgaag tggcctggcc
    42961 ggtgcgtgac gcggcgctgc gtcagcgggt gatcgacgag tgcctggtgc cgtatctcca
    43021 cgaccggcgc gaagcctgga tgctgaactc cgacggcagc ttctcgcgcc tcggcagcaa
    43081 tggagccagt gcccagcagg cgctggtggc ccgctacaag tcgggcagcc ggaggacctg
    43141 acgcgatgga cctgatcctg tggcgccacg cggaagcgca catcgtgaag gacgggcaga
    43201 ccgatctcga acgcgcgctg accgccaagg gggaacgcca ggcccagcgc atggccgact
    43261 ggctgaacca gcggctcgcg cattccacgc gcatccttgt cagcccggcg ctgcgctgcc
    43321 agcagacggc gaaggcactg ggccggtcct tcaagacgat tgacgcgctg tcgcccgatg
    43381 ccgccggtga tgcgctgctg aaggccgctc gctggcccga atcccccgac cccgtgctgg
    43441 tgatcggtca ccagcccacg ctggggctgg tggctgcaca cctgctggcc gacacgccgc
    43501 aggagtggtc gatcaagaag tgtgcagtct ggtggttgcg tcaccgcgat cgcgagggca
    43561 acacgcaagt cgtgctgcag gcggtgcagt cgcccgattg cctctgatcc cggacccgga
    43621 tcagtagccc gacgagcgcg gcagctcgag ctccagaggc ccgccgcgct gccatgcggc
    43681 cgcctcctcg gtgagaaggt aatgtgtgcg gggatggatt tcaatccagc ccggctgcca
    43741 gctcagttcg gccaccgagc ccttgcgccg caaggcgatg gcatggttgt cgaccgtccc
    43801 gcgggcgtgg cacttgatga tcgccagccg cagacacatg gcctgccacg cgaaatcggg
    43861 ctgcatcagg ctggcttcga ccttgcgcag accacctcgc tgcgccagca ccagttcggc
    43921 cagacggcgt tgctgcgact gggagaatcc cggcgcatca acgttgccca gcatgtacgc
    43981 gctgtgacgg tgatggtcgt ggtgcgacac catcatcccg atctcgtgca gcgcgcaggc
    44041 ccactgcagt tcgcgccgcg catcgcgccc ggcatcgggc tgcacgttgt tgtagagggc
    44101 ttccgcgacc tggccgaccc gctgcgcctg ggcttcttcg acgaggaagc gttgctggag
    44161 ctcgcgcacc gagctgtcgc gcacgtcctc gccgtcgttg ggttgctggc cggcattgag
    44221 tcgctcgtcg agatcgaaga tcacgccctg acgcagcgcg ccgcgcgccg gcagcagggc
    44281 atcgataccg aagtgcgtgg ccagcgtgta caggatggac aggccaccgg cgatgatcgc
    44341 gcggcggtct tccttcaggc cggccagcgc aagccggtcg acgcggcctg cggccaggca
    44401 ctgctccatc agccagcgca gcccgccggg cgtgatgcgg ccatcggtca cgcgattggc
    44461 cgagagcagc tgcgagaccg cgccgacggt acctgatgac cccagggcct cgacccagtg
    44521 cgtgggcgca aagggttcca gcgcttcttc gagctcggcg cccgccgcca cctgcgccgc
    44581 acggaaggcg gcctgggtgt accggccatc cccgaagtac ttcatcgaca ggctcacgct
    44641 gccgacctgg aacgactcgg cgcgcagcgg cttgcgtcct tcgccgagga tcatctcggt
    44701 cgatcgccca ccgatgtcga tcaccagccg aggctgttcc gaaggctgca gccgagccac
    44761 gcctgcgtag atcagccgcg cctcctcgcg tccggagatg acctcgatcg ggtacccgag
    44821 cacggtctcg gcgcgagcca ggaaggcatt gcggttgcgc gcctcgcgca gcgtctgggt
    44881 cgcgaccgcg cgcacctgcc atggagcgac gccctgaagc cggtgggcga aacgcgacag
    44941 gcacgacagg ccgcgcagca tggcctcttc ggtcagcaga ccgttctcgt cgaggccacc
    45001 gccaagtcgg acggtctcct tcaggtaatc gatgcgccgg tagcgagtgc cgtcgagctg
    45061 accgagttcg agccgaaagc tgttcgaccc catgtcgatc gcggccagct gccctcgcca
    45121 gcgtgtcccc gccttgtcgg gcggagcgga ccgcgcagag aggatggaat tcatcgagga
    45181 attgtcgggg ataaccgtga caggatcggt gctgtggcca gggcaggcat tgcgaatgca
    45241 ggaacgctct ggcggccgtg tcggcgaact ttcaccgcgc ctggcaatcg acccgcaggc
    45301 tcgagtgttt caacccttca acgaggtgtc accatgttca ggaacgatgt cgagagtctg
    45361 tgcccgacgc gtggctacag ccgccgtgat ttcgtgcaga ccgcggtggg cagcggtttt
    45421 gccgttgcgg tgctgccggt cggggctcag atcattcgga ccgacaccgc aggcctgctg
    45481 gccggccccg tgacgatccc tgtcggtggc ttcgagctgc ctgcctatcg tgcggtcccc
    45541 gcgcaggcca gcgctccgcc ggtcgtgctg gtgatcagcg agatcttcgg tgtgcacgag
    45601 cacatcgccg acgtggcgcg ccgattcgcg aagctgggct acctggccat cgctcccgag
    45661 ctgttcgtcc gacagggcga cgcgaaggcg gcgaccgaca tggcccggct gatctcggaa
    45721 atcatcgcca aggtgcccga tgagcaggtc atgggcgacc tcgatgccac cgtggcctgg
    45781 gcaaagacgc agggtggcga cacctcgagg ctcggcatca ccggcttctg ctggggcggc
    45841 cgcatcacct ggctgtacag cgcccgcaat ccggcggtca aggccgccgt cgcgtggtac
    45901 ggccggctgg tcggtgcggc ttcggcgctg cagccgatgc accccgtcga catcgcggcg
    45961 cagttgcacg caccggtgct gggcctgtac ggcggagccg acgccggcat tccacaggcc
    46021 agcgtcgatc agatgaaggc cgcgctgtca gccggctcac caggtgccag gcggtcgcag
    46081 ttcgtggtct atccggacac gccgcacgcg tttcacgccg actaccggcc cagctaccgc
    46141 aagccggcgg ccgacgatgg ctggcagcgt tgcctggact ggttgcgggc caacggcgtc
    46201 ggctgatcct ggtgctgggg tcagcgcacc acaagcacgg gaatcgtgct gtgcgtcagc
    46261 accttctggg tttcgctgcc cagcagcagt gcagtgactc cgcggcgtcc atgcgaagcc
    46321 atgaccagca gatcgcagtg ggccgctttc gcgaggtcga tgatcgcctg ccaagggtgg
    46381 tcggcctcca ccgtgtgcga ttcacaggtg acccccgccg cggcgcaggc ctgctgcacc
    46441 tgctggactc gcgccgccga gatgcgctgc tgcgcatcga agtactcctg cggcggcacc
    46501 ggctgcatct cggacatcgc gttgtatgga aacggctcct tgacgctcat ggcgacgatc
    46561 tggccgccca gcgatttcgc cagtccgatc gcggtctcga tcgccttggc ggtgatgtcc
    46621 gagccgtcgg tcggaacgag gatgcgcttg aacatgacgt gctccttgcc ttgaatcagg
    46681 gttggccttg ctcagccacg agcgaccggg cgcgtggggt ccgacgacca ttcgctccac
    46741 gatccagggt agagcgcggc gccgtcgagc cccgcaacgg tcattgccag caggttgtgg
    46801 catgcggtga ccccggagcc gcattgatgc accacctcgg acggggggcg ggtgccgagc
    46861 agcgccagaa acccggtgcg caactcgtcg gccggcttga accgaccgct ggcggccagg
    46921 ttctccttga agaaacgatt gcgcgcgccg ggtatgtggc cggcctgagc gtccagcggc
    46981 tccacatcgc cgcgaaatcg ttccggcgcc cgcgcgtcga tcagcgcgat cccgccgagc
    47041 tgcgattgca gtgcggctgc gtcgagcgtg cgcatcggcg acgaggccat gccgcccacc
    47101 ggataagccg gttgcggcgt cacagctgcc acggcgccgg cttcgaccgg acccccgctg
    47161 tcctgccagg cggcgaatcc accgtcgagc accgccactg gcgcatggcc cagccagcgc
    47221 agcatccacc acagtcgcgc ggcgaacatc gcgccctggc ggtcatagat gatgacctgg
    47281 gtggtcggcg tgatgcccag ttgtcctgcg gttgcggcga acacgtcggg ctcgggcagc
    47341 gggtggcggc cgttgcgccc ggtcttctgg ccgcacaggt cgcgatcgag gtgcacatgg
    47401 tgcgcacggg gcaggtgagc ctcgaggaag gtgcgttcac cggccgccgt gtctgccaga
    47461 tcgaagctgg catccaggat caggaccggg gcgccgctgt cgatcagcga tcgaaggtcg
    47521 ccggctgcga tcagcgtcgt gtacagcgtg gccgaggggt tcatggctcg acgttcaggg
    47581 tcgacgagtt cagcagtcgt tgcatcccgg cggacgggcg ctcggggcag gcctgcaaat
    47641 cggactccat cgcttcgcga taccaggtgt ggaactgctc catgccgtct tccatcgggc
    47701 tctggtaggg gccggactcg tggtccccgc gttgccacag ggccgcgcgg ccctgatcca
    47761 tgcgcaaggc aatctcgtcg tcctcgatgc aggtctcgag gtacgcggcc tgttgcgtgt
    47821 ccatgtactc gcgctcgaac gcagcgatct cttcggggta gtagaactcg accacgttgg
    47881 tcgtgtggcg tggtccgcgc gggtacagcg tcgaaaccac cagcacgttc ggataccact
    47941 cgatcatcag acccgggtaa taggtcagcc agatcgcgcc gtgcttgggc ggacgcccgt
    48001 cgtggtgctc ctgctgatag cgcaggacct cgttgtgcca gcggctgtag acgtcggagc
    48061 cgggcttgcc cagggcctcg cccgacgacg tggcgacgcc gaccgtctgg accgaatact
    48121 cggagcccat ctcccagcgc aggtcatcgg tcgtgacgaa gtttccgaga cccgggtgga
    48181 atggaccgac gtggtagtcc tcgaggtaga cctcgatgaa cgtcttccag ttgtagtcgc
    48241 actcgtgcac atggacccgg tcgagtacat ggccggaaaa atccagatcg gcacgtggcc
    48301 ccagcgcagc caggtcggct gcgatgtcgc ggccgttgtc ctcgaacacc aggccgttcc
    48361 agctgcgcgt cttgtagttg ttcaggtgga ggcacggtga ctcggtgaag tgcggcgcac
    48421 cgatcaactg gcctttcagg tcgtaggtcc agcggtgaag cggacagacg atgttgctgt
    48481 gcgtgttgcc ccgcccatgc aggatgaggg cctgccggtg gcggcagacg ttggagatca
    48541 gttcgacgct tttcggtgtg cgcaccagca cccggccgtc gccttcctgg ggcagcgtgt
    48601 agtagtcgcc gggattcggc acggacagct catgccctag gtagcgcggc ccgtggcgaa
    48661 acagcgtgtc ggcctcgcgc tggaacagtg cgtcgtcgaa atacgtcgac ggggccagtg
    48721 gcagggcgtt gtgcctggcc ccggttctgg tggggcgccc cgcgccgggc gtcagggcgc
    48781 cgcgcgcgac atgcagatcg gacatggtcc ttcgggtctc ctcggagagg gtggctggtg
    48841 gtcgctggtt caccgggcgt tcggcccgtc ggatgatccg gctcgcgcga gtcgcctgac
    48901 ggatgcccgg cggatggagg agatccccat cttcttccgg actcgtattt taggtggcga
    48961 cgccgcgggg gggacttgat ccatctcagt gatccggaaa cgcctgcgcg ggctggcgcg
    49021 ggtgcctcgt accccttcag cgctcatgcg ggtcggctgt ctacaatctg acggctcaga
    49081 gaccgcccat ggccaagaca tcatccaccg ttgcgaaccc tgtctctgcc caggccggca
    49141 ccgctgcctt gcccgacaac tacgaggctg cgttgtccga actggagcga ctggtcgggg
    49201 cgatggaggc ggggcaattg ccgctggatc aactgctcga caggtaccgt cgcggcgccg
    49261 agctgctgaa tttctgccgt cagcgcctgg aagcggtgga gcagcaggtg aaggtgctcg
    49321 aagacggcca gctcaagccc tggaatccga cctgatgacg tcgctgaccg acttcgactc
    49381 ctgggcgcgc tccgaactcg tcgctgtcga ggaatgcctg ggactctggc tgccggccga
    49441 cgcgccagcc ggattgggca cggccatgcg ctacggcgtg ctcgatggtg gcaagcgact
    49501 gcggccgttg ctggtgctgg cggcatccca tgcggtgggc ggtgcgcgca tcgcgtcatt
    49561 gcgcgcggcg tgcgcggtcg agctcatcca tgcctactcg ctggtgcacg atgacatgcc
    49621 ctgcatggac aacgacgtgc tgcggcgcgg caagccgacc gtgcacgtgc agttcggcga
    49681 agcgcaggcg atgctggctg gcgacgccat gcaggcgctg gcgttcgaag tgctgacgcc
    49741 cgcttcctgt gatgccgatg cccgatcgat accgcctgcg cttcaggctc gactctgcgg
    49801 ccttctttcc cgggcggctg gccactcggg catggcgggc gggcaggcca tcgatctcgc
    49861 cagcgtcggc cgcccgctcg acgagcgcag cctgcgcgac atgcactatc gcaagaccgg
    49921 agcgctgctg cgggccagcg tgatgatggg cgcggcctgc ggcgaggtcg atgcgggcgg
    49981 atgggcggcg ctcgaggtct acggcgactc catcggactg gctttccagg tgatcgatga
    50041 tgtgctcgac gtgacgcagc agtccgatgt cctgggcaag acggcgggca aggacgtcga
    50101 tgccaacaag ccgacctatg tcagcctgat gggtctgggg ccggcccgcc ggcatgcctt
    50161 cgagttgcgc gaccaggcga aatcggcgct gtcgagttcg ggtctgcgcg atacggtgtt
    50221 gcttggcctc ctggccgaca gggtggttga acgggacagt tgacgatgaa aaatgccttg
    50281 ctccaatcga tccagtcgcc ggccgacgta cgccggctgt cgcgcagcga attgcatctg
    50341 cttgccagcg agctgcgcga tttcctgctg cacagcgttt cgcagacggg tggtcacctg
    50401 tcatcgaacc tcggcacggt cgagttgacg gtcgcgctgc acaaggtctt cgatacgccg
    50461 aatgaccgca tcgtctggga cgtgggtcac cagacctatc cgcacaagat cctgaccggc
    50521 cgccgtgatc gcatgggttc gctgcggcag ctcggtggca tcagcggttt tccgcgccgc
    50581 gacgaaagcg agtacgacgc cttcggcacc gcccactcgt cgacctcgat ctccgccgcg
    50641 ctgggcatgg cggtcggcgc gcgcatgaag ggcgagcatc gcaaggcgat cgccgtcatc
    50701 ggtgacggcg cgatgagcgg cggcatggcc ttcgaggcgc tgaacaacgc cggagtggcc
    50761 ggtgccgacg tgctggtcat cctgaacgac aacgacatgt cgatctcgcc gccggtcggc
    50821 gcgctgaacc gctacctggc gcggctgatg agcgggcgct tctatgccaa tgcacgtgac
    50881 acggccaagt ccgttctgaa gaacgcgccg ccgctgctcg aactggcgcg tcgattcgaa
    50941 tcgcacgcca agggcatggt cgtgcccggg acgatcttcg aggagttcgg cttcaactat
    51001 gtcggcccga tcgacggcca cgacctcgac gcgctcatcc ccacgctcga gaacctgcgc
    51061 aacctgaaag ggccgcaatt cctgcatgtc gtcaccaaga aagggcaggg ctaccacctg
    51121 gccgagaacg atccggtggc gtaccacggc ccgagccggt tcaacccggc cgtgggcatc
    51181 acgcctgccg ctcccggcaa gccgacgttc acccaggtgt tcggcacctg gctttgcgac
    51241 atggccgagg cagacacccg gctggtgggc atcacaccgg ccatgcgcga aggctcgggc
    51301 atggtggagt tcgagagccg cttcccgcgg cgctatcacg atgtcggcat tgccgagcag
    51361 catgcggtga ccttcgccgc cgggctggcc tgcgaaggcc tgaagccggt ggtcgcgatc
    51421 tactcgacgt tccttcagcg cgcctacgat caattgatcc acgatgtcgc catccagaat
    51481 ctgccggtgg tgtttgcgct cgaccgtgcc ggtctggtgg gtgctgacgg cgcgacgcac
    51541 gccggcaact acgacatcgc ctacctgcgc tgcattccca acatgagcgt gatgaccccg
    51601 tccgacgaga acgagtgccg tcagttgctc tacacggcct ttctgcagga ccacccgact
    51661 gccgtgcgct acccgagagg caagggcgtc ggcgtcgagg tgcatcaggc catgacggcg
    51721 atcccgctcg gcaagggaga gatccgtcgc atggcgagcc cttcgagcgg ctcgcactgc
    51781 agacgcatcg cgatacttgc cttcggcacg ctgcttcacg ccgcgctaga cgctgccgag
    51841 cgcctcgatg cgacggtggc caacatgcgc ttcgccaagc cgctcgacat tgaactggtc
    51901 tgcgagctgg cgcgcagcca cgatgcgctg gtcaccatcg aggacggcag cgcgatgggt
    51961 ggtgcgggat cggcggtgct cgaagcgctg cagcaggccg ggctcatgat gccggtgctg
    52021 gtgctggggt tgcccgacga gttcatcgag catggcgacc ccgcggttct gatgtcccgc
    52081 tgcggcctcg actcggcagg gatcgagcaa tcgatcctga aacgctttgg cggccggccg
    52141 gcactggtcc gccccgcggt tcatcaatga ccggtcgagc cggcacgcgt cactgcagtg
    52201 tcggtgtcgc gtgggctgac gatgtaccgg ctgctggcag gccgcgcgaa tccggtctcg
    52261 tgcgagccaa ggtctgcgag ggccgctcgg cgaactgccg gcggtagtcg ctggcgaact
    52321 ggctcaggct ccagaatccg ttcctgccgg cggcgtcctg cacggacgtt gccgcacccc
    52381 tcagcaggtc gcgccgagcc gcgttcaggc gcaacgtgcg caggaagtcc accgggctgg
    52441 agccgaccac cgtctcgaag gcgtactgca aggtgcgtcg gctgacgtgc aggtgctcgc
    52501 acagctcggg taccgtggga accgcgtggg gatgcatgcg cacctgatcg caggcgcgac
    52561 gcaccagact gcggcgtcga tcgaagctca gtccctcgtg cccttcgacc agcggggtcg
    52621 ccagcaccga caagagtctg tctagcaccg ccgattgcgc gaaacggcgg ctctgcgcgt
    52681 ggtcgtgact gctcgcgccg gagcctgcct cggccagcag cagcgccagc ccgcggtgca
    52741 gttgcgcgtg ggcggctgga tcgcaggcga accagcccgg atgactcagg gccgccaggt
    52801 tcaacggctg ccccagcgtg tttgctgcct ccttcagtgc acttcgcgac gcgacgatgc
    52861 cgaagatctc gtgctggttc ggtgtgagaa gttcgaattc ggcgccgctg ccgctgacca
    52921 tcacaccgcc ggctccgacc acctgccctt cgatgcgcga gccgtccggt gccatcgtga
    52981 tgccgcacca gatcgtgtcg ggccggatct ggcaggtctg tcgtaccgcg tggctggtgc
    53041 gttcgcggaa cacctggatc tcatcgaacc agagctccga cagttcgccg tgaaagggac
    53101 ctcgggacag ctggtcgtat tgctgctgcc agcccgtcag gttggcggca tgcgtgacca
    53161 cgtcatccga ttgcgaccag cgcatggccg ccggaccgga aacggcttgc cgaggtgacg
    53221 atgagacgga atcagcaaca agtgcgcgca tggtggactc ccgcaaggtt tgccgaattg
    53281 cgataacgcc aacgcggaaa tccgccttag cctgaccttc gtcgacgcga ctcaggtgcg
    53341 agcaagcgtc gggc
//
LOCUS       DAOORV010000011        44544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_943_length_44544_cov_7.600058, whole genome shotgun sequence.
ACCESSION   DAOORV010000011 DAOORV010000000
VERSION     DAOORV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 44544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44544
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_943_length_44544_cov_7.600058"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1065
                     /gene="holB"
                     /locus_tag="PKV56_02940"
     CDS             28..1065
                     /gene="holB"
                     /locus_tag="PKV56_02940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOX66795.1"
                     /translation="MPWLAAPLRLALQTQRAHALLVHGPQGVGQFEMALTLANAWLCE
                     SVSSDIATRPCGHCASCLLVRARSHPDLLVLVPEALREELGWNADDASEEGGSGRGEA
                     SGKRKPSKDIRVEEVRRIVTFAQTTAARSLGKVVVVYPAERMNGVSANALLKTLEEPP
                     GNLRFVLAGSSVDALLPTIRSRCQAVRLGIPETSVAAAWLQAQGITQPEVLLAAAGGQ
                     PEEALRWFREGLDARAWLALPEQLARGDAAGLPAWPLPRVVDMLFKVCHDAMRSAVGA
                     APRYFPAASMGPKVCSDALTRWYRELCRIASHAEHPWQAALTIEMLVMQAQTALAVPK
                     SAGGRSPPWIN"
     gene            1070..1465
                     /locus_tag="PKV56_02945"
     CDS             1070..1465
                     /locus_tag="PKV56_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX66796.1"
                     /translation="MTDPVSRPPSTAANPVLAGSRPSVIQLVFREKGALYAAYIPVLT
                     DGGLFVPTTREYRLGEDIYLLLSLPEDNQRYPVAGKVAWITPANASGGRTQGVGVRFP
                     ADEKTRVLRTRIEELLGTAISSPKPTQTI"
     gene            1498..2298
                     /locus_tag="PKV56_02950"
     CDS             1498..2298
                     /locus_tag="PKV56_02950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX66797.1"
                     /translation="MYVDSHCHLSFPDLRARLTDIRAEMQSAQVDRALCICTTLEEFD
                     VVHALAMAYDNFWASVGVHPDNEGVREPSVDDLVALASRPRVVALGETGLDYFRLNGR
                     SVDEMEWQRERFRVHIRAAHQTGLPLVIHTRSSSADTLAVLTKEGGHRLSGVFHCFTE
                     TMDVARAALDLGFYISFSGIITFKNAEDLRSVARFVPLDRCLIETDSPYLAPAPFRGK
                     TNTPALVPWVAKQIAELKGVPVEEIARETSANFEHLFGLTAGCPDEKS"
     gene            2285..2944
                     /locus_tag="PKV56_02955"
     CDS             2285..2944
                     /locus_tag="PKV56_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOX66798.1"
                     /translation="MKNRRKYFQIGFYLFVLNGFGAVKAGSYEDFFTAIRRDHRNNIE
                     ELLKRGFDPNTVDEQGQPGLILALKLESFKVSEALIDSPMLKLEATNPAGETALMLAS
                     LRGQPELVERLLKRGANVNRPGWSALHYAATAPDELTLQKLLERGAAVDARSPNGTTP
                     LMMAAQYGTSESVELLLARGADVNLRSDLDHSAADFARRVGRDRTADGLARRMRKPPA
                     P"
     gene            3024..3275
                     /locus_tag="PKV56_02960"
     CDS             3024..3275
                     /locus_tag="PKV56_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66799.1"
                     /translation="MLAILELIHQLSGRFQMHTRPSIANPFSMMINPEAVLQALQQSD
                     SLAGLQAQVFRPLDKPLLGSKMPEDVVAYDRRIDQGRRN"
     gene            complement(3445..4083)
                     /locus_tag="PKV56_02965"
     CDS             complement(3445..4083)
                     /locus_tag="PKV56_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOX66800.1"
                     /translation="MKRRTLLMASPMLLGGCAGSLLPKPSRSPTSFTLDDGSPAATPV
                     STGDGAPVLLVDMPRAAPGFDSKRMIYARRPQELEAYAFSEWVDTPARLLAPMLARSL
                     QSGGAFGAVMLAPSAALGAMRLESELIRLQQDFSVYPSIVRLTVRASLLDAATRAVIA
                     SREFDEREASVSEDASGGAAAAQRAIQRLLTALSAFFSQQARRRPSAAASGE"
     gene            complement(4080..5018)
                     /locus_tag="PKV56_02970"
     CDS             complement(4080..5018)
                     /locus_tag="PKV56_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOX66801.1"
                     /translation="MDDKVNYSLVGAFVLLLGAALVAAVLWLAAGLGGKQAVETYQAF
                     IQESVAGLNVDAPVKYLGVDVGKVSRIEIDPLNSRQVRLRFVINRGTPIKQDSLAVLK
                     TQGLTGIAYVELSGGSAGSLALVAGADGLPPTIPFKLSLGARLENVLTSVLGNVDRVS
                     NNLNAIFDADNRAALKATLADTATLAHALASQQGALGAGIADAAKTAHLTARAGEQLG
                     PSLERIASGAQAVERMADVAAAASVKAGAAAEAAASGVQRLGSDTLPEMARLMSELSQ
                     LSMSLRHLAEQTAHTPNSLLLGRPHPSPGPGEGASP"
     gene            complement(5030..5770)
                     /locus_tag="PKV56_02975"
     CDS             complement(5030..5770)
                     /locus_tag="PKV56_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX66802.1"
                     /translation="MQSISTRFGSHQVHEGLDLSVRRGEILGLAGGSGSGKSVLLREM
                     IMLQRPSSGSVRLFGRDVATLDAAQTQALRQRWGVMFQRGGLFGSITVRRNVGLPLRE
                     FSHLDDALIDEIAEWKLALTGLPPETGLKLPGELSGGMLKRAALARAIALDPELLFLD
                     EPTSGLDPASADGVDELIRSTHALYGSTTVIVSHDLDLLWQVCDRVAVLGEGRVLALG
                     TMQELSQIEHPLVSSYFTDRRGRGRASA"
     gene            complement(5802..6905)
                     /locus_tag="PKV56_02980"
     CDS             complement(5802..6905)
                     /locus_tag="PKV56_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX66803.1"
                     /translation="MPDVRPDTPATLTPGPAGEWLLGGAWTVHDLGRVGQGTAAPTAR
                     AVLDGSQLLAIDSAGAWVLQNWLRTGGATPSLRAWPERARSLMQQVLAGSAQPVSQAQ
                     RPGLLQGLGLRAVEAWQQTLAFLEFIGQAAVAAASAITHPKRFHWRAVLREIEIGGFD
                     ALVIVGLMSFLLGVVVAYQGADQLRHYGANIFVVELVGYAMLREFAPLIAAIIIAGRS
                     GSAYAAQIATMVVTEEIDAMRTIGIDPLQRLVLPRIVALAVALPLLTVFADMTGVFGG
                     MVMARSQLGIGFGEFASRFGEVMNGSDLLVGVGKALIFSVIVATVGCFQGFRTRGSSD
                     SVGRQTTLSVVQSIFLVIVADALFSVAFNVLGL"
     gene            7177..7398
                     /locus_tag="PKV56_02985"
     CDS             7177..7398
                     /locus_tag="PKV56_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66804.1"
                     /translation="MTITTHSARITGPIPYLSSSGAEDHIPIGPCLVEQVNDRSIDII
                     WGDQGHHSAALPVEVIEAAQDCGNLLLLD"
     gene            7477..9048
                     /locus_tag="PKV56_02990"
     CDS             7477..9048
                     /locus_tag="PKV56_02990"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HOX66805.1"
                     /translation="MSSTSKAYLVGGGIGSLAAAAFLVRDGGMPGSNISILEAGPIMG
                     GSLDGSGDAERGYSMRGGRMLTTDNYECTWELFKSIPSLAKPGLTVFEETLAFNEVHV
                     SHARARLVDARRAKVPVASMGFSMHDRLELLKLSSADESTLGTTRITDHLSPAFFETE
                     FWFMWATTFAFQPWHSAVEFRRYLHRFMLEFSRIETLAGVKRTVLNQYDSLVVPLQRW
                     LESQGVRLITDCTVTDLDHHTVDGRFAVTGLHCTLKGRSETMLLGDGDLVFVQNGSMT
                     DASSLGSMSEAPAKRTRAPNGAWTLWEKLADGRPSFGRPAVFNSCVAQSNWASFTVTL
                     KDTAFFDQMQRFSGNEAGTGGLVTFKDSNWLMSIVLAHQPHFANQPADVQVFWGYGLF
                     PDRVGNFVAKPMADCSGAEILQELCGHLRFDLDVMASANCIPCRMPYITSMFMPRAPG
                     DRPPPVPPGTRNFAFISQFVEVPLDTVFTVEFSVRAAQIAVYELLGLDRKVPPVTPHD
                     HSFHARFEALLKAIK"
     gene            9078..10112
                     /locus_tag="PKV56_02995"
     CDS             9078..10112
                     /locus_tag="PKV56_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOX66806.1"
                     /translation="MSQSSTVNRRIRLHSRPVGAPTAADFCMDNDSIPTPVDGEVLLR
                     TLYLSLDPYMRGRMSDEPSYAEPVALGDVMVGGTVSRVEVSRNADFRVGDLVAGYAGW
                     QDYALSNGLGLRGLAHEEPHPSRALGVLGMPGLTGYMGLLDIGRPVFGDTVVVAAASG
                     AVGSVVGQVAGIKGCRVIGIAGGEDKCRYVMDELRFDQCIDHHTEDLPGRLAAACPNG
                     IDVYFESVGGAVFDAVLPLLNPGARVPVCGLISTYNSVAPPPGPDRLGMLMGTLLRKR
                     IRMQGFIVSDDYGDRFSEFSGAMGPWVEDGRVQVSEEIVFGLENAPEAFIGLLKGRNF
                     GKLVMQLAHE"
     gene            complement(10187..11398)
                     /locus_tag="PKV56_03000"
     CDS             complement(10187..11398)
                     /locus_tag="PKV56_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX66807.1"
                     /translation="MLFNVFMLALGSVQRNLLRSFLTILGIVIGVSAVITMVTLGQGA
                     TQSIQMQISSLGTNLLMVRPGQRGFGGGGGSVPQFTEADAEAIVSQIGGVATAVPQGT
                     TSATVVANGRNWVTTVYGSTNDWFDTSNWKLASGRIFAHDEQVAGSATCVIGETVRRE
                     LFGGSAGQTGLGEQVRVKQFSCTVIGILAAKGQGGMGDQDDAVVLPLNTLQRRVTGNR
                     KVNTLLVSMEEGSDSGPLKAGLRDLLRERRKLAEADDDNFNIFDTQQLAETLSSTTQV
                     LTTLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGLRLAVGALESEVLLQFLIEAVV
                     LSGLGGLVGVVIATGASWGLARLMDVPYLFDPTINLLSMVFSAVIGVVFGYFPARRAA
                     QMDPIEALRHE"
     gene            complement(11398..12138)
                     /locus_tag="PKV56_03005"
     CDS             complement(11398..12138)
                     /locus_tag="PKV56_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66808.1"
                     /translation="MSEPLIQLRGVTRRYGSGASELMALKGIDIDIAAGEFVAIMGPS
                     GSGKSTAMNILGCLDTPTSGQYLFRQTHVESLSRDQRARLRRRYLGFVFQGFNLLART
                     SAQENVELPLLYRGDGAAARRTAATRALQSVGLGGWEDHTPAQLSGGQQQRVAIARAI
                     VTEPAVVLADEPTGNLDTRRSHEIMGLLLALNRDHGITVLMVTHEPDMAAYARRMVHF
                     VDGGISRDEPNPNPVTAAPAELAATEST"
     gene            complement(12185..13528)
                     /locus_tag="PKV56_03010"
     CDS             complement(12185..13528)
                     /locus_tag="PKV56_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX66809.1"
                     /translation="MNTAQPADTTNPPPAAASDAQTDLKTLLAEPTARVWYRRPVTWA
                     IVAAVVLATGAAWWWWASRAAGTAPSYTTQAVTRGNLTLTVTANGTIQPTRSINIGSE
                     LSGTVLKVNVDVNDTVKKGQVLVVLDTSKLRDQILRSQAALAASIAKVAQTTATIREA
                     AAGLKRLEDVAILSGGQVPSKAELDTGRATLARAVADDASAQAGVNDARAALSTDQIN
                     FSKASISAPADGVVLTRTVDPGNAVAASLQAVTLFTLAEDLARLRLWVYVDEADVGAV
                     KVGQDATFTVSAYASRQFPARITRVGFGSTITDNVVTYLTYLDVDNGDLSLRPGMTAT
                     ASIIATKRSDVLLVPNTSLRFTPTAPASDNKAKGGVMSSLTPRMPGSNTRRSAAAGAS
                     TSAAKQVWVLPKDGKGPPVSVAVTPGISDGRQTEITGGDLQVGMLVITDQKKAAP"
     gene            complement(13525..14985)
                     /locus_tag="PKV56_03015"
     CDS             complement(13525..14985)
                     /locus_tag="PKV56_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOX66810.1"
                     /translation="MAWSTATILLLAVLPGCALRLPPADSSGLGTMTVPTRWTLPAGS
                     ETSGATSPTSLSSWWQRFDDPTLDALVTQSQQANTSIRSAQSALRQARALRDLASAAL
                     WPGIGYSASVQHGTAGGHSAGSSFQAGLDASWVPDVFGANRSALEASRATLDATAADL
                     GDMQVAVAAEVGLNYLLLRTAQARLGIARDNLASQRETWQITQWRQQAGLVTVLEAEQ
                     ARAAAEQTAALLPALQTSIEQTRHALAVLLGEPPATRSPLFATLDESAPIPRAAEELA
                     LAIPAETLRQRADVRAAESQVRAAWARVAQADAGRFPGFALGGSFGWNAATLGSLANG
                     SSVVSSLLASVTGPLFDAGSARAQVRAQEAAYEQARVAYQATVLAALKDVEDALVALR
                     GDRQRLVSLSSASDAAARAATLARQRFSSGLVDFQVVLETQRTQFSAQDSVASATADI
                     GADQVRLFTSLGGGWRDDRDDDALASSTRTQRTSRP"
     gene            complement(15076..15501)
                     /locus_tag="PKV56_03020"
     CDS             complement(15076..15501)
                     /locus_tag="PKV56_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66811.1"
                     /translation="MVPPAQREMAAGAYTLQRRSQRQRSHGAMLRLDTAPAGLGTNAS
                     PSSQIRPGNPAGAQRLPGPDPELWCVMFDAIVESMSTIIDVAPDARSTLAEPHRAEQL
                     RATLMRCIGDLRQLRASAVPESAPQPGASTIESTADPVR"
     gene            complement(15737..17161)
                     /locus_tag="PKV56_03025"
     CDS             complement(15737..17161)
                     /locus_tag="PKV56_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOX66812.1"
                     /translation="MPRLSDAGQHAGAAPPRYRYRHVWTACVLGLAGCSSSPAVAPDP
                     GLGPATVLHLQGAQGNLSPSRSKAILQRLSRGVEETGIFDHHLAREQAIVGSPLTTGN
                     RVLLLQDGPATYAAMLAAIVGARDHINMETYILDDDDVGRRFARALIDRQRDGVQVNL
                     IRDSFGTLGTPAAFFQQLRDAGIQVLEFNPLNPLMARKEWELNQRDHRKLLIVDGRTA
                     FLGGINISSVYSGGSFSQGMRARPAGSPAWRDTDLQLEGPVVAELQSLFLATWTAQRG
                     PSLAPRAYFPPAVSAGREVVRAIGSSPDEAVSTIYATLISAIASAETSVRLTNAYFAP
                     DPRLLAELEAAAARGVDVTLILPSQSDAWLVFHAGRASYERLLRAGVKIHERRGVILH
                     SKTALIDGVWATVGSTNLDWRSFVHNHELNAVVLGADFGGQLQAMFESDLAASEAITL
                     EEWRRRPLQFRLQEWLGKLWDYWL"
     gene            17367..17588
                     /locus_tag="PKV56_03030"
     CDS             17367..17588
                     /locus_tag="PKV56_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66813.1"
                     /translation="MNAIRMLGIALILSGALGLAYGSFSWTKETHEAKVGPIELSLSE
                     QQTVNVPVWAGLGAIVVGGALLLLAGKRT"
     gene            17649..18656
                     /locus_tag="PKV56_03035"
     CDS             17649..18656
                     /locus_tag="PKV56_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66814.1"
                     /translation="MRQLIIGIWLLCCAVTSATAQVSVGLGINLPGVSIGINLPAYPD
                     LVRVPGYPVYYAPGLQSNFFFYDGMYWVYEDDNWYASSWYNGPWGLVGPQSVPLFVLR
                     IPVRYYRSPPSYFHDWRADAPPRWGEHWGNDWERQRRGWDQWNHRSAPRPAPLPAYQR
                     KYSGDRYPQQIDRQQQLRGQNYRYQPRDTAVRQHEGQQQQRQKERQAQRPPQQKQPAA
                     PPPAQREEKPAPAVRGTRPSDFERGSPPTRSEKAVERPAQRPAAPEQRSPARPPQQQQ
                     QPPRTERAPEQREQRAPGPGQRDPSRREEQPRQEPSRGQGPGQGQGQGNDRERGEGRG
                     R"
     gene            18783..19280
                     /locus_tag="PKV56_03040"
     CDS             18783..19280
                     /locus_tag="PKV56_03040"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOX66815.1"
                     /translation="MLEPGPVVLLTTTHKGTPDVMTMSWHTMMEFEPPLVGCVVSRND
                     FSFAALKSSRQCAINIPTADLAPQVVGCGNTTGQRLDKFKAFGLTPVPATVVAPPLID
                     ECYANLECRVVDTRLMNRYDFFVLEVVKAWIDPACKSPRTLHHRGRGRFMVAGETIKL
                     ASRMK"
     gene            19304..20230
                     /locus_tag="PKV56_03045"
     CDS             19304..20230
                     /locus_tag="PKV56_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66816.1"
                     /translation="MSGHIGPTAGVSRLLAMVALALGLCGHAAVVQAGGQPSSASTAT
                     RLHATRAELQPRLRTSSFGEPLVLISRELPGRMEGDAYADVDHPFAAVGDAFRTATGI
                     CELLFMHLNVRSCTPSAGAGGDRLTLVAGPKRSTGISMQDSISYTMRTEVADATHLRV
                     TLSAAQGPWSTHDYLMVFEAVPVDAAHSFVHFGYSYAYGALARMAMGSYLATAGRSKI
                     GFTVEGTDADGRPKYVQGVRAAIERNVMRYYLALQARFSITTGSAEERSQARLRAWFA
                     LTERHAAQLHEYDIDEYLQEKRRDLARMAPGT"
     gene            20230..21471
                     /locus_tag="PKV56_03050"
     CDS             20230..21471
                     /locus_tag="PKV56_03050"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOX66817.1"
                     /translation="MDSIPAPEQRHGSATHVLALNSGSSSLKFGLYRVGPTRIDRLVT
                     GAAEMIGDAAAVIHAENTLGQGPLREAIPMDDQRAAIIRIGQLLSDLEAPAPDAIGHR
                     VVHGGPQLREHCLIDEAVLQLLEASTAFAPLHTPAALALIRFAARHFPGLPQVACLDT
                     TFHARMPSVASTLPLPAELRSAGIQRYGFHGLSCESIVGQLAGTMPDRLIIAHLGNGA
                     SITAVKHGTSIDTSMGMTPTGGLIMGTRSGNLDPGVLVYLMRDMRLDCAGIENLVDHR
                     SGLLGISGISGDMRSLHEAAAESADARLAIEMFSYAAAKEIGAMGTVLNGADMIVFTG
                     GIGEHDGLVRAAICARLSGLGVELDEGRNRLGRGSIGRAGSRCEVQVLASQEDEQIAR
                     HAWSLCRGAAKSTGRAHAGSG"
     gene            complement(21476..22276)
                     /locus_tag="PKV56_03055"
     CDS             complement(21476..22276)
                     /locus_tag="PKV56_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOX66818.1"
                     /translation="MLRNVKDLETYAISASDGEIGPVTDVYFDDDAWAIRYFVVDTGS
                     WLSSRKVLISPISVHQPHWLAQSLPVSLTRKQVRNSPDIDTDKPVSRQNEEQVLGHYG
                     YPHYWGGAGLWGDGRHPQSMAPGHAHQEDDRAGRERELEAGLRDEKARHSNDDPHLRS
                     CNAILGYGIHATDGDIGHVAGFLVDDETWAIRFLIVDTSNWWIGHKVLIAPPWIQGVH
                     WAERSVSVALDLESIRSAPAYDATVDWSNEQDLSLHRHYGRSNYRQVV"
     gene            22479..24971
                     /locus_tag="PKV56_03060"
     CDS             22479..24971
                     /locus_tag="PKV56_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HOX66819.1"
                     /translation="MLDEPPGSRLAAPCSPAHRPAAAAGLCSTRPRCTCDASLRTTEV
                     RTMKTETLSPDLLRRLDAYWRAANYLSVGQLYLYDNPLLREPLTMAHIKPLLVGHWGT
                     VPGQNFIYAHLNRAIRKYDLNMFYVSGPGHGGAALVANVYLEGTWSEVYPGVTQDLDG
                     MKKLFKQFSFPGGISSHVAPTTPGSINEGGELGYSLSHAFGAVFDNPDLIVACVVGDG
                     EAETGPLAASWHANKLLDPVNDGAVLPILHLNGFKISNPTLLARIGREELEQYFLGCG
                     WAPIFVEGDDPATMHQLMAAATDQAIEQILQIQDVARNKNVRTRSRWPLIVLRSPKGW
                     TGPQEVDGLQIENTFRSHQMPLLVSPDLPGHVKLLEAWMKSYRPQELFDESGRLMPEL
                     AELAPAGERRMGANPHANGGLLLRELQVPDFREYAFPVAAPGNSEAHDTGVLGSFLRD
                     VLRMNRNQRNFRIFGPDETVSNLLGAVFEATSRQWDAERLANDEFLQTDGLVVDSMLS
                     EHQCQGWLEGYLLTGRHGLFNSYEAFIRIVDSMFSQHAKWLKVTRELPWRRPIASLNY
                     LLASHVWQQDHNGFTHQDPGFIDHVVSKKADIVRVYLPPDANCLLSVTDHCLRSRNYV
                     NVVIASKHARPQWLTIDEAVAHCTAGIGMWTWAGNDQEANPDIVMACCGDTPTLEILA
                     ATAMLREHLPELKVRVVNVVDLMKLQPAHEHPHGLDEADFDSLFTADRPVIFAFHGYP
                     SLVHRLTHGRANRNLHVHGYQEEGTITTPFDMRVQNELDRFHLVQHAVDCLPQLGSRG
                     AHLKQMTRDKLIEHKHYIEQHGQDLPEIRNWK"
     gene            25051..25257
                     /locus_tag="PKV56_03065"
     CDS             25051..25257
                     /locus_tag="PKV56_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66820.1"
                     /translation="MSRIEQLEMNARREQLSADVKELVERYRSIFSWDVPEVDEALSD
                     RLILGALRLAIDDIETGLPDGPLH"
     gene            25254..25949
                     /locus_tag="PKV56_03070"
     CDS             25254..25949
                     /locus_tag="PKV56_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOX66821.1"
                     /translation="MNGEHGIAPESTPGAALSIYFIRHAETAWSLSGQHTGRTDLPLT
                     PRGESMACHLAPALKDIPFSLVLTSPRLRARTTCRLAGLAGSGSSAARAEPMLAEWDY
                     GDFEGLCTDEIRQLQPGWDIWRDGCPGGELPAETGARADALLEQLCGLTGNVALFSHG
                     QFGRVLAARWIGLPVFEGRHFAIDPASIGILGFENAHPERRVISMWNARAADQPVPVD
                     ARATAPVIDARID"
     gene            complement(26007..27080)
                     /gene="tal"
                     /locus_tag="PKV56_03075"
     CDS             complement(26007..27080)
                     /gene="tal"
                     /locus_tag="PKV56_03075"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOX66822.1"
                     /translation="MNEPTRQLNDLGQQLWLDHISRPLLRSGTLQRYIAELSITGLTS
                     NPTIFGLALAGDAYDASIRALDAKGMAIEELFFELAIEDVTQAADLFRPIFDASEGAE
                     GWVSLEVSPLLAHNTARTIQAASALHERAARPNLFIKIPGTAEGVSAVEEAIFDGVPV
                     NVTLLFSTEHYLAAAHAYMRALERRLAARLDLGVASVASLFVSRWDAGVKEEISPPFH
                     NRLGVAIAMRTYRAYRELLASDRWRRLAAAGARPQRLLWASTATKDASAPDTLYVQSL
                     AAPDTVITLPEMLLLAFADHGQVGAVMPADGGYADAVLEEFRREGVDDDALAQRLQRE
                     GVDSFADSWHAMLSRIREKTAAR"
     gene            complement(27104..27406)
                     /locus_tag="PKV56_03080"
     CDS             complement(27104..27406)
                     /locus_tag="PKV56_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66823.1"
                     /translation="MSDRARRIDELVDDHQLFRFQWHHDWWLPMFQLDKSGPSLATAP
                     RRVIAELGDAFDGWAMAIWFVQPNSWLEGHSPIERLEANLPDVLAAARADRFVATA"
     gene            complement(27758..28513)
                     /locus_tag="PKV56_03085"
     CDS             complement(27758..28513)
                     /locus_tag="PKV56_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HOX66824.1"
                     /translation="MTLDTTHSHRADHGKRSFTKAAGRHGENHRSDRIGWLRAAVLGA
                     NDGIVSTASLVLGVAAAGADHRTILMTSVSGLIAGAMSMAAGEFVSVHSQADTESADL
                     AREQAELTKDPVGERRELAGIYVSRGLDRPLADQVAAELMAHDALGAHARDELGLSET
                     TAARPIQAALASAASFAVGAVLPIGVVVTAAPQHLIVWVTLASLGFLAGLGAVSAQLG
                     GARVWTGTWRVAFWGAVAMAVTAGAGALFGAVA"
     gene            complement(28620..29420)
                     /locus_tag="PKV56_03090"
     CDS             complement(28620..29420)
                     /locus_tag="PKV56_03090"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HOX66825.1"
                     /translation="MNKMLVSIFDNETAAEAGLQALRKLHADGDITLYAAGVIAKDDK
                     GAVSVKKSMNEGATGTGAGLAVGSLIGLLGGPIGVAVGAVTGAVAGAVRDFWVAGVGL
                     DFIEETEKSLRPGKVALVAEVEEEWVNPVDAAIEAAGGRVFRRTRTEVAEAQFDHDIS
                     AFRSEIEELEAEAGYASGAAKEKLQSTLTHAKSRLDGLVHRAKQRVEMLKQEAEAKGD
                     VIKAQLSQSRGEVQAKIEARVKHVKSVYHARGVKLGQAWALTREALAL"
     gene            complement(29468..30154)
                     /locus_tag="PKV56_03095"
     CDS             complement(29468..30154)
                     /locus_tag="PKV56_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOX66826.1"
                     /translation="MKKNIRSQALAMLAVAATFCAPAPLWAQVAGGTTTIDTSIIEST
                     QIAMGWSVKKTLMGKTIHNETGEKVGQIEDLIIAPDKNVSYVIVGAGGFIGIGRHDVA
                     IPVTQIQDRGGKLVMPGATKELIKSLPEFTYADNSASRDRFVAAADRDIAKGKAKVSE
                     LEKKAGAAATDAKARIDLQISALQVDVKSAEAKLTEMKQATAARWKEFEAGVSAATAR
                     LRKSIDTAAS"
     gene            30707..31360
                     /locus_tag="PKV56_03100"
     CDS             30707..31360
                     /locus_tag="PKV56_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOX66827.1"
                     /translation="MKTDSQLQQDVTAELKWEPSVHAARIGVEVQDGVVTLSGQVDSY
                     AEKWSAERAAQRVSGVNAMAVELKVELTGLSKRTDADIGRAVENVLEWTSSLPPGAVK
                     VMVEGGWVTLTGNVDWKYQKQTAADSVRNLMGVTGVSDQIAVRPPVSSIAVMSDIESA
                     LTRAAIADAGKISVAVHGCDVTLSGTVHNWAERETATNSAWGTPGVRNVVDMMTLAR"
     gene            complement(31754..33133)
                     /gene="pufC"
                     /locus_tag="PKV56_03105"
     CDS             complement(31754..33133)
                     /gene="pufC"
                     /locus_tag="PKV56_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome PufC"
                     /protein_id="HOX66828.1"
                     /translation="MNKLSTILLNLVLLSVAALLSGCERPPIEVVQHGYRGTGMAEIY
                     NPRTLATQAKLNEVPAPIAAATPGGPPAAQIYQNVQVLNDLTVGEFSRLMVAMTAWVA
                     PEQGCTYCHNAQNFASDENYTKQVARKMLQMTRKINSDYKSHVAATGVTCYTCHRGNN
                     IPQNVWFKAPHEARTDALIGDKAGQNTVSVVANKSSLPVDPFTPLLLGSEDIRVNGTT
                     ALPTGNRHSIKQAEWTYSLMTHMSTSLGVNCTYCHNSRSFANWAESPPQRVTAWYGIR
                     MVRDLNTQHMEPIEKLFPPNRLGPTGDVAKANCSTCHQGAFKPMYGVSMLKDHPELAA
                     QRPALGASAPVAANATAESATVFFAVGSSALTEEAAKSLEVLTQGLSGSSSAKAVISG
                     FHSAAGNVKQNQELAKQRALAVLDLLKARGIGTDRAVLERPRSAEANVSGEDPKARRV
                     EVAIRQATG"
     gene            complement(33130..34113)
                     /gene="pufM"
                     /locus_tag="PKV56_03110"
     CDS             complement(33130..34113)
                     /gene="pufM"
                     /locus_tag="PKV56_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit M"
                     /protein_id="HOX66829.1"
                     /translation="MAEYQNIFTSVQAVGPIHHGIELPHGNAPRTGDEPLIVHLLGRI
                     GNAQIGPVYLGSLGVASLMLGMLAFNIIGFNMLASVDWNPIQFVRQLFWLGLEPPPPQ
                     NGLAIPQSMNQGGWWLLAGFLTTCSVFLWFARTYRRARQLGLGTHVAWAFLAAIWLFL
                     VLGFIRPILMGSWGEAVPFGIFPHLDWTAAFSIRYGNLFYNPFHMLSIAFLYGSTLLF
                     AMHGATILAVSRFGGEREIEQITDRGTASERAALFWRWTMGFNATMESIHRWAWWFAV
                     LTPLTGGIGILLTGTVVDNWYLWAVKHHVAPMYPPVWAPVIDPATLQGVKP"
     gene            complement(34138..34962)
                     /gene="pufL"
                     /locus_tag="PKV56_03115"
     CDS             complement(34138..34962)
                     /gene="pufL"
                     /locus_tag="PKV56_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit L"
                     /protein_id="HOX66830.1"
                     /translation="MATLSFERKYRIRGGTLVGGDLFDFWVGPFYVGFFGVTTIFFAA
                     LGTALILWGAALGGTWNVWQINIAPPDLKYGLGFAPLREGGLWQLITVCAIGAFSSWA
                     LREVEICRKLGIGLHVPIAFSFAIFAYVTLVVIRPVLMGAWGHGFPYGILSHLDWVSN
                     TGYQYLHFHYNPAHMLAISFFFTTTFALSLHGALVLSSTNPKKGEVVKSPEHEDTFFR
                     DAIGYSIGTLGIHRLGLFLALSAVFWSAVCIVISGPFWTRGWPEWWGWWLNLPIWH"
     gene            complement(35079..35285)
                     /gene="pufA"
                     /locus_tag="PKV56_03120"
     CDS             complement(35079..35285)
                     /gene="pufA"
                     /locus_tag="PKV56_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, alpha subunit"
                     /protein_id="HOX66831.1"
                     /translation="MWRIWRLFDPLRAMVVQGIFLFGLAVMIHLVLLSTSTFNWIDGP
                     NARAKASQAQHAALPAGVTSPVAK"
     gene            complement(35296..35520)
                     /locus_tag="PKV56_03125"
     CDS             complement(35296..35520)
                     /locus_tag="PKV56_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66832.1"
                     /translation="MAESTNLTLQQILKKDSKVYLAIFAVGFCLFLLIAVFAQVMTWQ
                     WRSWLPGAEAEKSLIGGVKGAVYTFMSHLL"
     gene            complement(35545..35691)
                     /gene="pufB"
                     /locus_tag="PKV56_03130"
     CDS             complement(35545..35691)
                     /gene="pufB"
                     /locus_tag="PKV56_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HOX66833.1"
                     /translation="MAERKGSISGLTDEEAQEFHRFWVQGFIGFTAVAVVAHFLVWAW
                     RPWL"
     gene            complement(35835..37313)
                     /gene="bchZ"
                     /locus_tag="PKV56_03135"
     CDS             complement(35835..37313)
                     /gene="bchZ"
                     /locus_tag="PKV56_03135"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Z"
                     /protein_id="HOX66834.1"
                     /translation="MLVLDHDRAGGYWGAVYVFTAIKGLQVVIDGPVGCENLPVTSVL
                     HYTDALPPHELPIVVTGLAEEQLGREGTEGSMRRAHAALDPDLPSVVVTGSIAEMIGG
                     GVTPSGTNIQRFLPRTIDEDQWQCANRAMFWLWSEYGMKKVPARKPYAERPAGEKPRV
                     NIIGPCYGTFNCPSDLAEIRRLVQGIGAEVNMTFPLGSHLADVSRLVEADVNICMYRE
                     FGRMLCEALDRPYLQAPIGLHSTTKFLRKLGELLGLDPEPFIEREKHTTIKPIWDLWR
                     SVTQDFFGTASFAVVANDTYTRGIRHFLEDEMGLPCQFAISRLPGAKTNNEEVRQLVK
                     DKTPLVMYGSYNERIYLAECGGGSMMKANFIPASFPLPIIRRHTGTPFMGYSGATYIV
                     QELCNALFDALFHILPLGTDLDRVEPTLARVDGQAPSPWDLDAQELLDEYVQTEPVLV
                     QISAAKRLRDRAERDARRAGEERVTAARVLQSREAMRQGEPA"
     gene            complement(37313..38899)
                     /gene="bchY"
                     /locus_tag="PKV56_03140"
     CDS             complement(37313..38899)
                     /gene="bchY"
                     /locus_tag="PKV56_03140"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Y"
                     /protein_id="HOX66835.1"
                     /translation="MSKTIPIVAANSASATPPTAPGVSSTTLEGDGMGCHAGGDQLLA
                     AAKSAGKSEVLAQYAKDYPKDPKAGPHDQPQSMCPAFGSLRVGLRMRRTATILSGSAC
                     CVYGLTFTSHFYGAKRSVGYVPFNSESLVTGKLFEDIREAVHKEADPAKYDAIVIINL
                     CVPTASGVPLQILPKEINGVRIIGIDVPGFGVPTHAEAKDVLAGAMLKYARGEVQAGP
                     VQAPRNGRSDKPTIALLGEVFPADPVIIGMMLEPMGLASGPVVPTREWRELYAALDCT
                     AVAAIHPFYTASIREFEAAGRPIVGSAPVGHDGTEAWLQAIGQATNTSQANIDAAKNR
                     FLPAIRGALAKTPIKGRITLSGYEGSELLVGRLLVESGADLRYVGSACPRTAWNEADL
                     QWLESKGVQVRFRASLEQDLAAMAEFQPDLAIGTTPVVQAAKAQSIPALYFTNLISAR
                     PLMGPAGAGSLATVINAAIGNKARFEQMKEFFGEVGEGHSSGVWEPSNGVPTDRPEFK
                     ADQRRQAEKLARKRKAEEMI"
     gene            complement(38913..39938)
                     /locus_tag="PKV56_03145"
     CDS             complement(38913..39938)
                     /locus_tag="PKV56_03145"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
                     /protein_id="HOX66836.1"
                     /translation="MSTAPSTLSPVRDAPVTIMMKDLRHEASVEPPAVATGPVTKETQ
                     IIAIYGKGGIGKSFTLANLSYMMAQQGKKVLLIGCDPKSDTTSLLFGGKACPTIIETS
                     SKKKLAGESVAIGDVCFKRDGVYAMELGGPEVGRGCGGRGIIHGFETLEKLGFHDWGF
                     DYVLLDFLGDVVCGGFGLPIARDMCQKVIVVASNDLQSLYVANNVCSAVEYFRKLGGN
                     VGVAGMVINRDDGTGEAKAFAEHVGIPVLSTIPANDDIRRKSASYEIIGRPDSEWGPM
                     FAQLAENVGTSLPMRPKPMSQDELLGLFSAASVGRDVVLEPATQFDMCGKTESKKATL
                     EVVYDEI"
     gene            complement(39935..40864)
                     /gene="bchC"
                     /locus_tag="PKV56_03150"
     CDS             complement(39935..40864)
                     /gene="bchC"
                     /locus_tag="PKV56_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HOX66837.1"
                     /translation="MAVVLERPEQLVLSRLDLSEATDEDVTVDIEWSGISTGTERLLW
                     SGRMPAFPGMGYPLVPGYESVGRVAKAGSMSGRSVGERVFVPGAKCFGDVRGLFGGAA
                     AHVVLPGARTIPFAEHLAEQGVLLALAATGYHAHSMAGLKQPDLIVGHGVLGRMIARL
                     AVVAGSSPTVWETNPARREGAAGYAVIDPAEDTRRNYSCICDVSGDSSLLDTLISRLA
                     PGGEVVLAGFYDEPLSFVFPPAFMREARIRIAAQWLPSDLVAVKDLAESGRLSLDGLI
                     THRSEAAQADTAYRTAFSDPACLKMILDWRSCS"
     gene            complement(41025..42203)
                     /locus_tag="PKV56_03155"
     CDS             complement(41025..42203)
                     /locus_tag="PKV56_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOX66838.1"
                     /translation="MSAFGRSEPQALLEPDPSLLDRLKDRWFSWRDGLVGSRRFRRWA
                     AAFPLTRPIAQRRARGLFDLVAGFVYSQVLLACVRLRLFDMLAQEPQTLSALAPQLGL
                     SEDAALRLLRAAVSLDLVEERSRGRFGLGPLGAPLVGDAAIASMVEHHADLYVDLADP
                     VALLRGEARTSALASYWPYAAADAPGSLDAGRVSPYSALMSASQTLVSDEVLDAYSFD
                     KHRRLLDVGGGEGTFLSAVAQRAPHLQLVLFDLPAVAERARVRFAEQGLAGRTTVAGG
                     SFFSDPLPAGCDVISLVRVIFDHDDARALQILKAVRRAMPDGGTLLLTEPMAGTRGAE
                     AMGDAYFGFYLLAMGKGRSRSASQLTELLTAAGFGDVRLLRNPMPLQTQVIVASAGRV
                     "
     gene            complement(42200..43081)
                     /locus_tag="PKV56_03160"
     CDS             complement(42200..43081)
                     /locus_tag="PKV56_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX66839.1"
                     /translation="MKPITRIEQSLAAAIASGEEEGCPPKLAGAIRHAVFPGGARIRP
                     QLCLAVAQACGDDDPLLSEATATAIELLHCASLVHDDLPCFDDAPTRRGRASVHFAFG
                     ESLAVLAGDALIVLAFQTLGAAATKSPLRLPMLLRTIGRATGMPHGIVAGQAWECEPR
                     VSLADYQRAKTGSLFAAATIAGAQAAGADSAPWRALGEWLGEAYQVADDIRDVASDPL
                     SLGKPTGQDVALCRPSAARELGLEGAIHHFDRLVAAAIEAIPPCPGAVQMRALVRFEA
                     ERLVPKAMAEEVVRVAA"
     gene            complement(43231..44241)
                     /locus_tag="PKV56_03165"
     CDS             complement(43231..44241)
                     /locus_tag="PKV56_03165"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOX66840.1"
                     /translation="MKMAVVGAGAIGGYLGARLALSGEEVTFIARNENLAAINERGFR
                     LILEDGIVKHAHGVRAVRDPADAGLQDVVLLTLKAHQVHQVLPQLRALFGPETVVVTM
                     INGVPWWYFHRLAGPYEGLGLTSVDPGGVIARHIETDRVIGSVVYPAAELVEPGVVRV
                     IEGNRFTLGELDGTRTQRVEAISQVLMRAGFKAPVSRDIRAEIWIKLWGNLSFNPISA
                     LTHATLEGICRHPLARGLAADMMLEAQGVAERLGVVFKISLEQRLAGAAAVGAHKTSM
                     LRDVELGRALELEALVGSVVELGRITGMPTPVINAIYAATALLSETLSAAHGRLEVQP
                     SG"
     gene            complement(44267..>44544)
                     /locus_tag="PKV56_03170"
     CDS             complement(44267..>44544)
                     /locus_tag="PKV56_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HOX66841.1"
                     /translation="PREVDEILMDHPAVAQVVCFGVPHPKLGEEVAAVVVLKEGVPAT
                     ERELQAFVSQRLADYKVPRKILFMDELPKGATGKLQRIGLAQRLGLG"
BASE COUNT         7813 a        14720 c        14339 g         7672 t
ORIGIN      
        1 agcgggccgg ccgcaaccac tgcgccgctg ccctggctgg ccgcgccact gcgactggcg
       61 ctgcagaccc agcgcgctca tgccttgctg gtgcacggac cgcagggtgt cgggcagttc
      121 gagatggccc tgacgctggc gaatgcctgg ctgtgcgaaa gcgtttcgag cgacatcgca
      181 acgcgaccct gcggacattg cgcgagttgc cttctggtgc gggcccgctc gcaccccgat
      241 ctgctggttc tggtgcctga agcgctgcgc gaggaactgg gctggaacgc cgacgatgcg
      301 agcgaggagg gtggttctgg caggggagag gcctccggca aacgcaagcc cagcaaggac
      361 attcgggtcg aagaggttcg acgcatcgtc accttcgcgc agaccacagc ggctcgatca
      421 ctggggaagg tcgtcgtcgt ctacccagcc gaaagaatga acggcgtgtc tgccaacgcg
      481 ctgctgaaga cgctcgagga gccgcccggc aaccttcgtt tcgtcctggc aggttcttcg
      541 gtcgatgcgc tcctgccgac catacgcagc cgttgccagg ccgtgcgcct cgggattccc
      601 gagacgtcgg tggcggcggc ctggctgcag gcgcagggca tcacccagcc cgaggtcctg
      661 ctcgctgccg cgggcgggca accggaagag gccttgagat ggttccgcga gggcctcgat
      721 gcacgagcct ggctcgcctt gcccgagcag ttggctcgtg gagacgccgc gggtttgccg
      781 gcgtggcctt tgccgcgcgt tgtcgacatg ctgttcaagg tctgccatga cgccatgcgc
      841 tcggcggtcg gcgcagcacc gcgctatttc cctgcggctt cgatggggcc gaaagtctgc
      901 agcgacgccc tgacacgctg gtaccgggag ctgtgccgca ttgcaagtca tgccgaacat
      961 ccgtggcagg cagcgctgac cattgaaatg ctcgtgatgc aggcacaaac tgcgctggcc
     1021 gtgccgaagt ctgcaggcgg ccggtcgccg ccttggataa actgacgaca tgaccgatcc
     1081 tgtctcgcgc ccgccttcca ccgctgccaa ccccgtgctg gcgggtagcc gtccgagcgt
     1141 gatccagctg gtgttccgtg aaaagggggc gctctacgcc gcctacatcc cggtgctcac
     1201 cgatggaggc ctgtttgtgc cgaccacgcg ggagtaccgg ctcggcgagg acatctatct
     1261 gctgctttcg ttgccagaag acaaccagcg ctacccggtg gccggcaagg tcgcgtggat
     1321 cacgcccgcc aatgcatcgg gcggcagaac ccagggcgtt ggtgttcgat tcccggcgga
     1381 cgagaagacc cgcgtgctgc gcacccgtat cgaagagttg cttggcaccg ctatttcgtc
     1441 gcccaagccc acgcagacaa tctgagtctg gctgcggccc tgctgccgct gcacaagatg
     1501 tacgtcgact cccattgcca tctcagtttt ccggacttgc gggctcgtct gaccgatatc
     1561 cgcgccgaga tgcagtccgc ccaggtggat cgggcgcttt gcatatgcac gacgctggaa
     1621 gaatttgatg tagtgcatgc gctggccatg gcttacgaca acttctgggc cagcgtgggc
     1681 gtgcatccgg acaacgaggg cgtgcgcgag ccgtctgtcg atgatctggt tgcgctcgct
     1741 tcgcggcccc gcgtggtcgc gctcggtgaa accgggcttg actacttccg actgaatggc
     1801 cgttcggtcg acgagatgga gtggcagcgc gaacggttcc gcgtgcacat ccgggccgcc
     1861 catcagaccg gcctgccgtt ggtgatccac acccgcagtt cgtccgccga cacgctggca
     1921 gtgctgacca aggagggcgg gcatcggctc agcggtgtgt tccattgctt caccgaaacc
     1981 atggacgtcg cgcgcgccgc gctggacctg ggcttctaca tatcgttttc ggggatcatc
     2041 acgttcaaga acgccgagga cctgcggtcg gtagctcgct ttgtcccgct cgaccggtgc
     2101 ctgatcgaaa ccgacagtcc ttacctggct ccggcgccct ttcgtggcaa gaccaacacg
     2161 cccgccctgg ttccctgggt cgcgaagcag atagccgaac tcaagggtgt gcccgtggag
     2221 gaaatcgccc gggaaaccag cgccaacttc gagcatctgt tcggtctcac cgccggctgc
     2281 cccgatgaaa aatcgtagaa aatactttca aataggcttc tatctatttg ttttaaatgg
     2341 attcggtgca gtcaaggctg gttcgtacga ggatttcttc accgcaatca ggcgagacca
     2401 ccgcaacaac atcgaagaac tgctgaagcg tggcttcgac ccgaacacgg tcgatgaaca
     2461 agggcagcct ggcctgatcc tggcgctcaa gctcgagtcg ttcaaggtgt ctgaggccct
     2521 gatagacagc ccgatgctga aactggaagc aaccaacccg gcgggtgaaa cagccctgat
     2581 gctggccagc ctgcgagggc agcctgagct ggtcgagcgc ctgctgaagc ggggcgccaa
     2641 cgtcaatcga ccgggctggt ctgcgctgca ctacgctgcc acggcacccg atgaattgac
     2701 gttgcagaag ctgctcgagc ggggggctgc ggtcgatgcg cgctcaccca acggcaccac
     2761 gccgctgatg atggccgcgc agtacggcac gtctgaaagc gttgagttgc tccttgcccg
     2821 gggagcggat gtgaacctgc gcagcgatct cgatcacagc gccgccgatt tcgctcggcg
     2881 cgtcgggcga gaccggaccg cggatgggtt ggcccgccga atgcgcaagc cgcccgcgcc
     2941 gtgacgagcg gatggtcgat agcctgacag ccttgtcatc ctgtttctga cgcttcatat
     3001 ggttacaaga cgactgccgg actgtgcttg cgatcctaga gttgatccat caactttcag
     3061 ggagattcca gatgcatacc cgcccgtcga tcgccaatcc gttttccatg atgatcaatc
     3121 ccgaagctgt gcttcaggcg ctgcaacagt ctgattcact ggctggtttg caggcacagg
     3181 tcttccggcc gctcgacaag ccgctgctcg gtagcaagat gccggaagac gtggtcgcgt
     3241 acgaccgccg catcgaccag ggccgcagga actgagttcg tgcgtgtcct cgctgtcata
     3301 cgtgaggctg tctgcccacg ttcaacagcg gtaacaccat ccatcaagta agtgactatg
     3361 tatattatgt aaacagtcgg atgagtgggg gtacggaaac aagctcccac cgccacaccg
     3421 tggaatgggc ttcaccgcca gccatcactc gccggaggcg gcggctgaag gtctgcgcct
     3481 agcttgctgg gaaaagaaag cagataacgc cgtcagcaga cgctgaatcg cgcgttgtgc
     3541 cgccgcggcg cctcccgaag catcttcgct cactgaagcc tcgcgctcgt cgaactcgcg
     3601 ggaagcgatg acggcgcgag tcgctgcgtc cagcagggac gctcgcacgg tcaggcgcac
     3661 gatgctcggg tacacgctga aatcctgctg cagacggatc agttcggact cgaggcgcat
     3721 ggcgccgagc gcggccgatg gagcgagcat cacggcaccg aatgccccgc cagattgcag
     3781 cgaacgcgcc agcatcggcg caaggagccg tgccggcgtg tccacccatt ccgagaacgc
     3841 gtaagcctcg agttcctgtg gtcgacgggc gtagatcatg cgcttgctgt cgaagccggg
     3901 ggccgcgcgt ggcatgtcga ccagcagcac cggagcgccg tccccagttg acaccggagt
     3961 cgcggcgggc gagccgtcgt caagggtgaa actcgtcgga gatcgtgagg gcttgggcaa
     4021 caagctgcct gcgcagccgc ccagcagcat cgggctggcc atcaacaacg ttcgtcgctt
     4081 catggcgagg caccttctcc ggggcccggg ctcggatgcg gccgaccgag caacaagctg
     4141 ttgggagtgt gggctgtctg ctcggccagg tggcgaagcg acatcgaaag ctgactcagt
     4201 tcggacatca gacgcgccat ctcgggcaac gtgtcggaac ccagacgctg aactccactg
     4261 gcagcggctt cggctgcggc ccctgccttc acgctggccg ccgcggccac gtccgccatg
     4321 cgctcgaccg cctgcgcgcc gctggcgatc cgctccaggg atggcccgag ctgttcgccg
     4381 gcccgcgccg tcagatgcgc cgtcttcgca gcatcggcga tgccggcacc cagcgcaccc
     4441 tgctgcgacg ccagcgcgtg cgccagggtg gcggtgtccg ccagcgtcgc tttcagcgca
     4501 gcccggttgt ccgcgtcgaa gatggcgttg aggttgttgg acacgcggtc gacattgccc
     4561 agaacgctgg tgagcacgtt ctccagtcgt gccccgagcg agagcttgaa cgggatcgtc
     4621 ggcggcaatc cgtcggcgcc agccaccagt gccagcgagc ccgcgctgcc accactgagc
     4681 tcgacgtaag cgatgcctgt caggccctgg gtcttcagaa ccgccagact gtcctgcttg
     4741 atcggcgtgc ctcggttgat gacgaacctg agccgcacct ggcgcgagtt gagcgggtcg
     4801 atctcgatgc ggctcacctt gcccacatcc acgccgagat acttgaccgg tgcatcgacg
     4861 ttcagccctg ccaccgactc ctggatgaac gcctgatagg tttccaccgc ctgcttgccg
     4921 cccagcccgg cagccagcca gagcaccgcg gccacaagcg cggcgcccag cagcaggacg
     4981 aacgcgccca ccagcgaata gttgaccttg tcgtccatgc tcgcgagccc tatgcgctgg
     5041 cgcggccgcg gccgcgcctg tcggtgaaat aggagctgac caggggatgc tcgatctgcg
     5101 ataactcctg catcgtcccc agcgccagca cacggccctc ccccagcacc gccacgcggt
     5161 cgcacacctg ccagagcagg tccaggtcgt gcgagacgat cacggtggtc gagccgtaga
     5221 gcgcatgcgt gctccggatc agttcgtcga ccccgtccgc gctggcggga tccagccccg
     5281 aagtcggctc atccaggaac agcagttccg ggtccagcgc gatcgcgcgg gcgagcgctg
     5341 cacgcttgag catgcccccg ctcaactcgc cgggcagttt cagaccggtt tcaggcggaa
     5401 gtccggtgag ggcgagcttc cattcggcga tctcgtcgat cagggcgtcg tccagatgac
     5461 tgaactcgcg caggggcagc cccacgttgc ggcgcaccgt gatcgaaccg aacagcccgc
     5521 cgcgctggaa catcacgccc cagcgctggc gcagcgcctg ggtctgcgca gcgtccagcg
     5581 tggcgacatc gcggccgaac aacctgaccg agcccgaact cgggcgctgc agcatgatca
     5641 tctcgcgcag cagcaccgac ttgccggatc cgctgccgcc ggccaacccc aggatctcgc
     5701 cgcgccgcac gctgaggtcg agcccttcgt gaacctggtg gctcccgaag cgggtgctga
     5761 tggactgcat ttcgatcaca ggaacttgcg cgccgtccac gctagagccc cagcacgttg
     5821 aaggcaacgg agaacagcgc gtcggccacg atgaccagga atatcgactg caccacgctc
     5881 agcgtggtct gcctgccgac actgtccgag ctgccgcgcg tgcgaaatcc ctgaaagcag
     5941 ccgactgtcg ccacgatcac ggaaaagatc agtgccttgc cgacacctac gagcagatcc
     6001 gagccgttca tcacctcgcc gaagcgactg gcgaattcgc cgaatccgat gcccagctgc
     6061 gagcgcgcca tcaccatgcc gccgaagaca ccggtcatgt cggcgaacac ggtcagcagc
     6121 ggcaaagcca cggccagtgc gacgatcctt ggcagcacca ggcgttgcag cgggtcgatg
     6181 ccgatggtgc gcatggcgtc gatttcctcg gtgacgacca tcgtcgcgat ctgcgctgca
     6241 taggccgagc ccgagcggcc tgcaatgatg atggcggcga tcagcggcgc gaactcgcgc
     6301 agcatcgcgt agccgaccag ttcgaccacg aagatgttgg caccgtaatg cctgagttga
     6361 tcggctccct gataggccac caccacgccg agcaggaacg acatcaggcc cacgatgacc
     6421 agcgcgtcga acccgccgat ctcgatttcg cgcagcaccg cgcgccagtg gaagcgcttc
     6481 gggtgcgtga tggcggaagc agcagccact gcggcctggc cgatgaattc caggaatgcc
     6541 agggtctgct gccaggcctc gaccgcccga agtcccaagc cctgcagcaa gcccgggcgc
     6601 tgcgcctgcg acaccggctg ggctgaaccg gcgagcacct gctgcatcag gcttcgcgcc
     6661 cgttccggcc acgcgcgcaa cgacggcgtc gctccacccg ttcgcagcca gttctgcagc
     6721 acccaggcac ctgcgctgtc gatggcgagc agctgcgatc cgtccagcac agcccgggcc
     6781 gtgggtgcgg cagtgccctg ccccacgcgt cccaggtcgt ggacggtcca cgccccgccc
     6841 agcagccact cgccagcagg gcctggggtc agcgtggctg gggtatcggg tcggacgtct
     6901 ggcattgatg cgggaggaca catgaagggg ctggcagggt tcctgtcgct gaggccgggc
     6961 catgccgcgg tgcagcgctc aatgtaggaa ccgccccgtg gccgctccga tagtccgaga
     7021 ccgggattcg ctgtaggccg acgccccatg cggtcgccgc gggcgtgaca tggccccggg
     7081 aacccgcccg agccgatgac acgaagcctg tgcgctggcg cacagaccgg cagtcgctga
     7141 agcagcagtc ttgcgtctcc tcaagcgaga cgcgtcatga ccatcacgac acacagtgca
     7201 cgcatcaccg gtcccatccc ctacctgtcc agcagcggag ccgaagacca catccccatc
     7261 ggtccctgcc tcgttgaaca ggtgaacgac cgctcgatcg acatcatctg gggcgatcaa
     7321 ggtcatcact ctgccgcgtt gcctgtggag gtcatcgagg cggcgcagga ctgcggaaat
     7381 ctcctgctgc tcgactgatt tcggcgcgcg attccaccag acggatcgag tccccggacc
     7441 gatcccggac agcccctctt ccaaggaatt tccatgatga gttcgacatc gaaggcctat
     7501 ctcgtcgggg gcggcatcgg atccctggcc gccgccgcat tcctcgtgcg cgatggtgga
     7561 atgccggggt cgaacatctc gatcctggag gctggcccga tcatgggggg cagtctggac
     7621 ggctccggtg acgccgaacg cggctactcg atgcgcggcg gtcgcatgct cacgacggac
     7681 aactacgagt gcacctggga gttgttcaag tcgatcccgt cgctcgccaa gcccggcctg
     7741 accgtgttcg aggagacgct ggcgttcaac gaagtgcatg tctcgcatgc acgggcccgg
     7801 ctggtcgacg cgcgccgggc caaggtgccc gtggcttcga tgggcttctc gatgcacgac
     7861 cggctggagc tgctgaagct gagcagtgcc gatgaatcca ccctcggcac cacccgcatc
     7921 accgaccacc tgtccccggc gttcttcgaa accgagttct ggttcatgtg ggccacgacc
     7981 ttcgcattcc aaccctggca cagcgcggtc gagttccgac gctatctgca ccggttcatg
     8041 ctcgagttct cgcgcatcga gacgttggcc ggcgtgaaac gcacggtgct gaaccagtac
     8101 gactcgctgg tggttccgct gcagcgctgg ctcgaatcgc agggcgtgcg cctgatcacc
     8161 gactgcacgg tgaccgacct tgatcatcac accgtcgatg gacggttcgc ggtcaccggc
     8221 ctgcattgca cgctgaaggg ccgcagcgaa acgatgctcc tgggagacgg cgacctggtt
     8281 ttcgtgcaga acggatcgat gaccgatgcc tccagtctgg gttcgatgag cgaggcacca
     8341 gccaagcgca cccgcgcgcc caacggcgcc tggacgcttt gggagaagct ggccgacggc
     8401 aggccctcgt tcggcagacc cgctgtcttc aacagctgcg tggcgcagtc gaactgggcg
     8461 tcgttcaccg tcacgctgaa ggacacggcg ttcttcgatc agatgcagcg cttcagcggc
     8521 aatgaagcgg gcaccggcgg actcgtcacg ttcaaggact cgaactggct gatgtcgatc
     8581 gttctggcac accaaccgca cttcgcgaac cagccggcag acgtgcaggt cttctggggc
     8641 tacggcctgt ttccagatcg agtgggcaac ttcgttgcca agccgatggc cgactgcagc
     8701 ggtgcagaga tcctccagga actgtgcggc cacctgcgct tcgacctcga cgtcatggcc
     8761 tcggccaact gcatcccgtg tcgcatgcct tacatcacca gcatgttcat gccgcgcgcc
     8821 ccgggcgacc gaccgccgcc ggtgcccccg ggcacgcgca acttcgcctt catcagccag
     8881 ttcgtggagg tgccgctgga cacggtgttc accgtggagt tctcggtgcg tgccgcccag
     8941 atcgcggtgt acgaattgct gggcctcgac aggaaggttc cgcccgtcac ccctcacgac
     9001 cattcgttcc acgcgcggtt cgaagccctg ctcaaggcca tcaagtaatc cagccactcg
     9061 accgcccacg ccaccccatg tctcaaagca gcaccgtcaa tcgccgcatc cgtctccaca
     9121 gccgaccggt cggggcgccc actgccgcag acttctgcat ggacaacgac tcgatcccca
     9181 ccccggtgga cggcgaagtc ctgttgcgca cgttgtacct gtcgctcgat ccctacatgc
     9241 ggggccggat gagcgacgag ccttcctacg ccgagccggt ggcgctcggc gacgtcatgg
     9301 tgggcggcac cgtgtcgcgg gtcgaggtgt ccaggaacgc cgacttcagg gttggtgacc
     9361 tggtggcggg gtatgccgga tggcaggact acgcgctgtc caacggcctc ggcctgcggg
     9421 gcctggcgca cgaggagccg catccttcgc gggcgctggg cgtcctgggc atgcctggct
     9481 tgaccggcta catggggctg ctcgacatcg gccgaccggt gtttggcgac accgtggtcg
     9541 tcgcagcggc gagcggtgcc gtcggatcgg tcgtcggcca ggtcgccggg atcaagggat
     9601 gtcgtgtgat cggcattgcg ggcggcgagg acaagtgccg ctacgtgatg gacgagttgc
     9661 gtttcgacca atgcatcgat caccacaccg aggatctgcc tggccgattg gccgccgcct
     9721 gtccgaacgg gatcgatgtc tatttcgaga gcgtcggcgg tgccgtgttc gatgcggtgc
     9781 tgcctctgct gaacccgggc gcgcgcgttc cggtctgcgg actgatttcc acctacaact
     9841 ccgtcgcgcc gccgcccggc ccggatcgcc tcgggatgct gatgggcacg ctgctgcgca
     9901 agcgcatcag gatgcagggc ttcatcgtct cggacgacta cggagaccgc ttcagcgagt
     9961 tttccggagc gatggggccc tgggtcgagg acggccgggt gcaggtcagc gaggaaatcg
    10021 tcttcggcct ggagaacgca ccggaggcgt tcatcggact gctgaagggc cggaacttcg
    10081 gcaagctggt catgcagctg gcccacgaat gacgttggat ccacgcggaa accgtcaaga
    10141 gtgtgtccgc gggatcaacc gcaccggact cggggccggc agccgttcac tcgtgtcgca
    10201 gcgcctcgat cggatccatc tgcgctgccc ggcgggccgg gaagtagccg aacaccacgc
    10261 cgatgaccgc cgagaacacc attgacagca ggttgatcgt cgggtcgaac aggtagggca
    10321 catccatcag ccgggccagt ccccaggacg cgccggtggc gatgacgacg cccaccagcc
    10381 caccgagacc cgacagcacc accgcctcga tgaggaactg cagcagcacc tcgctttcca
    10441 gtgcgcccac cgccaggcgc agcccgatct cccgggtgcg ctcggtcacg ctgaccagca
    10501 tgatgttcat gatgccgatg ccgccgacca gcaggctgac ggccgccacc gcgccgagca
    10561 gcgtcgtcag cacctgtgtc gtgctcgaca gcgtctcggc cagttgctgg gtgtcgaaga
    10621 tgttgaagtt gtcgtcgtcg gcctcggcca gcttgcggcg ctcgcgcagc agatcgcgca
    10681 ggccggcctt caggggaccg ctgtcgctgc cctcttccat cgacaccagc agcgtgttca
    10741 ccttgcggtt gccggtgacc cgccgttgca acgtgttgag cgggagcacc accgcgtcgt
    10801 cctggtcgcc catgcccccc tgccccttgg cagccaggat gccgatcacg gtgcacgaga
    10861 actgcttgac ccgcacctgc tcgcccaggc cggtctggcc cgccgatccg ccgaacagct
    10921 cgcgacgcac ggtctcgccg atgacgcagg tggccgagcc ggccacctgt tcgtcatggg
    10981 cgaagatgcg gccactcgcc agcttccagt tgctggtgtc gaaccagtcg ttggtgctgc
    11041 catagacggt ggtgacccag ttgcgtccgt tggccacgac ggtcgcgctc gtcgtgccct
    11101 gcgggacggc agtcgccacg ccgccgatct gcgagacgat cgcttcggcg tccgcctcgg
    11161 tgaactgcgg cacgctgccg cctccacccc cgaacccccg ctgacccgga cgcaccatca
    11221 gcaggttggt accgaggctg gaaatctgca tctggatcga ttgcgtggcg ccctggccca
    11281 gcgtgaccat cgtgatgacc gcactgacac cgatgacgat gcccaggatg gtcaggaacg
    11341 agcgcagcag gttgcgctga accgagccca acgccagcat gaagacattg aaaagcatca
    11401 ggtgctctcg gtcgcagcca gctcggcggg tgctgctgtc acgggattcg ggttgggctc
    11461 atccctggag atgccaccgt cgacgaaatg caccatgcgg cgcgcatagg cggccatgtc
    11521 gggctcgtgc gtgaccatca gcacggtgat gccgtgatcg cggttcagcg ccagcagcag
    11581 ccccatgatc tcgtggctgc gacgtgtgtc gagattgccc gtgggctcgt cggccaggac
    11641 cacggccggt tcggtgacga tggcccgcgc tatggccacg cgctgctgct ggccgcccga
    11701 cagctgcgcc ggcgtgtggt cttcccagcc gcccagaccc accgattgca gcgcgcgggt
    11761 cgcggcagtg cgtcgggcag cggcaccgtc gcccctgtag agcagcggca gctccacgtt
    11821 ctcctgcgcc gacgtgcgcg ccagcaggtt gaatccctgg aatacaaaac cgagatagcg
    11881 ccttcgaagc cgcgcccgct ggtcgcgtga gagcgactcg acatgcgttt gcctgaacag
    11941 gtactgtccc gacgtcggcg tgtcgagaca gccgaggatg ttcatggcgg tggattttcc
    12001 cgacccgctc ggacccatga tggcgacgaa ctctccggcg gcgatgtcga tgtcgatgcc
    12061 tttcagtgcc atcagttcgc tggcgccgct gccgtagcgc cgggtcacgc cgcgcaactg
    12121 gatgagcggc tcgctcacga gtcgttgccc gcgaggtgaa cggcgggccc ccgctgcagg
    12181 actctcacgg cgccgccttc ttctggtcgg tgatgacgag catgcccact tgcaggtcgc
    12241 caccggtgat ctcggtctgc cgcccgtcgc tgatgcccgg tgtcaccgcc actgaaactg
    12301 gcgggccttt gccatccttc gggagcaccc acacctgctt cgccgcggac gtgctggcgc
    12361 ctgccgctgc cgatcggcgc gtgttgcttc cgggcatccg tggcgtcagg ctcgacatca
    12421 caccgccctt ggccttgttg tcgctggcgg gcgcggtggg ggtgaagcgc agcgacgtgt
    12481 tgggcaccag cagcacgtca ctgcgcttgg tcgcgatgat ggaggccgtg gccgtcatgc
    12541 ccgggcgcag gctgaggtcg ccgttatcga cgtccaggta ggtcaggtag gtgacgacgt
    12601 tgtcggtgat cgtcgatccg aagccgaccc gggtgatgcg ggccgggaac tgccggctcg
    12661 cgtacgcgct gaccgtgaag gtggcgtcct gtcccacctt gaccgcaccc acgtccgcct
    12721 cgtccacgta cacccacagc ctgagcctgg ccagatcctc ggccagcgtg aacagcgtca
    12781 ccgcctgcag cgacgcggcc acggcgtttc ccggatccac ggtgcgggtg agcaccacgc
    12841 catctgccgg cgcgctgatc gaggccttcg agaaattgat ctggtcggtg gacagcgcgg
    12901 ctcgggcatc gttgacacct gcttgggcac tcgcgtcgtc ggcgacggcg cgcgcgagcg
    12961 ttgcgcgccc ggtatcgagt tccgctttcg agggaacctg accacccgac aggatcgcca
    13021 cgtcctccag ccgcttcagg ccggctgcgg cttcacggat cgtggcggtg gtctgggcga
    13081 ccttggcgat cgacgccgcc agggcggcct gcgagcgcag gatctggtcg cgcagcttgg
    13141 aggtgtccag caccaccagc acctgtccct tcttcacggt gtcgttgaca tcgacgttga
    13201 ccttgagcac cgtgccggag agttcgctgc cgatgttgat cgaccgcgtc ggctggatcg
    13261 ttccgttggc cgtgaccgtc agcgtgaggt tgccgcgcgt cacggcctgc gtggtgtagc
    13321 ttggtgccgt gcccgccgcc cgggacgccc accaccacca ggctgcgccc gtggcgagaa
    13381 cgaccgccgc aacgatcgcc cacgtcacgg gacggcggta ccagacccgc gcggtgggct
    13441 ccgccagcaa ggtcttcaga tcggtctggg catcggacgc cgctgccggg ggcgggttcg
    13501 tcgtgtcggc aggctgcgct gtattcatgg tcttgaggtt cgttgtgtcc tcgtgctgga
    13561 agcaagcgcg tcatcgtctc gatcgtcgcg ccagcctccg cccagggacg tgaacaggcg
    13621 cacctgatcc gcgccgatgt cggccgtggc gctggccacg ctgtcctgag cactgaactg
    13681 ggtgcgctgc gtttcgagca cgacctggaa atcgacgagc ccgctgctga atcgctgccg
    13741 cgccagcgtg gcagcccggg cagccgcgtc ggaagcactg ctcaggctga ccagccgctg
    13801 ccgatcgccg cgcaaggcga cgagcgcgtc ctccacgtcc ttcagtgctg caagcacggt
    13861 ggcctggtag gccacacgag cctgctcgta ggccgcttcc tgcgcccgca cctgagcacg
    13921 cgcggagccg gcgtcgaaga gcgggccggt cacgctggcc agcaggctgc tgacgaccga
    13981 agagccgttg gccagcgagc ccagcgtggc ggcattccag ccgaaggatc cgcccagcgc
    14041 gaagccggga aagcggcccg catcggcctg cgcgacgcgg gcccaggccg cgcggacctg
    14101 cgactcggcg gcgcgaacgt ccgcccgctg ccgcagcgtc tcggctggaa tggccagcgc
    14161 cagctcctcc gctgctcgcg gtatcggcgc agattcgtcc agcgttgcaa acagcggaga
    14221 ccgggtcgca ggaggctcgc cgaggagcac ggccagtgca tggcgcgtct gctcgatgct
    14281 ggtctgcaga gcgggcagca gcgcagcggt ctgctctgcg gcggctcgcg cctgctccgc
    14341 ctcgagcacg gtgaccagcc cggcctgctg gcgccactgc gtgatctgcc aggtctcgcg
    14401 ctgactggcg aggttgtcgc gcgcgatgcc caggcgggcc tgcgcggtgc gcagcagcag
    14461 gtagttcagg cccacctctg ctgccacggc cacctgcatg tcgcccaggt ccgcggcggt
    14521 ggcatccagc gtggcacggc tcgcctcgag ggcactccga ttggcgccga acacgtcagg
    14581 cacccagctg gcatccagac cggcctggaa gctgctgccc gcgctgtgtc cgcctgccgt
    14641 gccgtgttgc accgaagccg agtagccgat cccgggccac agcgcggcgg aagccaggtc
    14701 gcgcagagcc cgagcctgcc gcagggccga ctgggcgctg cggatgctgg tgttggcctg
    14761 ctgcgactgc gtcacgagtg cgtcgagggt ggggtcgtcg aagcgctgcc accagctgga
    14821 aagcgacgtg ggtgacgtcg cgccggaagt ctccgacccg gcgggcaggg tccagcgcgt
    14881 cggcacggtc attgtcccga gcccgctgga atcagcgggc ggcagtcgca aggcgcagcc
    14941 cggcagtacc gcgagcagca ggatcgtcgc ggtggaccag gccacggctg tgcgggcgaa
    15001 gggctgcagc aggagacgcg tgcgatgtat caaggcagca gggcgcagct caacgaccgt
    15061 gcccgaaaga atcgatcacc gaaccgggtc cgcggtcgat tcgatcgttg aagcacccgg
    15121 ttggggtgcg ctttccggca ccgcgctggc tcgaagctgt cgcaggtcgc cgatgcatcg
    15181 catcagggtc gctcgcagtt gctcggcccg gtgtggctcg gcgagcgtcg agcgcgcgtc
    15241 tggcgcaaca tcgatgatgg tcgacatgct ctcgacgatc gcgtcgaaca tgacgcacca
    15301 gagttcgggg tccgggccag gaagcctctg ggcgcctgcc ggattgccgg ggcggatctg
    15361 cgatgacggg gacgcgttgg tgccgagccc tgcgggcgcc gtgtccagtc gcaacatggc
    15421 gccatggctg cgctgtcgct gcgagcggcg ctgcagggtg tacgcacctg cggccatttc
    15481 ccgctgtgcg ggtggaacca agacgatcag tcctgcatgc atcggtctgg ggctttgcgt
    15541 cattttctgg atccctgttt cagtcgcggg cctcggggtg cgagcagaaa cccaccgggg
    15601 ctgacaccga atttgcgcca cgcagcccgg gtcatctgtg cgctgccaca cacagccgct
    15661 ggcggccctt gacgcgcgtc aagggcgaag aagccaccgc gccgcggtca agtcaacctg
    15721 gctgcaggcg cagggctcac agccaatagt cccagagctt gcccagccac tcctggagcc
    15781 ggaactgcag tggccgacgc cgccattctt cgagcgtgat cgcttcggat gccgccaggt
    15841 cgctctcgaa catcgcctgc aactgcccgc cgaagtcggc cccgagcacc acggcattga
    15901 gctcgtggtt gtgcacgaag ctgcgccagt cgaggttggt cgacccgacc gtggcccaca
    15961 cgccgtcgat cagtgcagtc ttcgaatgca ggatgacgcc tctgcgttcg tggatcttca
    16021 ccccggcgcg caggagccgc tcgtaggaag cgcgtcccgc atggaaaacg agccaggcat
    16081 cggactggct tgggaggatc agcgtcacgt cgacgccgcg ggcagccgcg gcttcgagct
    16141 cggccagcag gcgcgggtcg ggcgcgaaat aggcgttggt caggcgcacg ctggtttctg
    16201 cactggcaat ggcggaaatc agcgtcgcat agatcgtgct gaccgcttca tcgggtgagc
    16261 ttccgatggc gcgcaccacc tcccgccctg cgctcacggc aggtggaaag taggcgcgcg
    16321 gcgccaggga aggcccgcgc tgcgcggtcc aggtggcgag gaacagcgac tgaagttcgg
    16381 caaccaccgg cccctcgagt tgcaggtcgg tgtctcgcca ggccgggctg ccagccggcc
    16441 gggcgcgcat cccttggctg aaggatccgc cggaatagac gctgctgatg ttgatgccgc
    16501 ccaggaaagc cgtgcgcccg tccacgatca gcagcttgcg gtggtcgcgc tggttcagct
    16561 cccactcctt gcgcgccatc aacggattga gcggattgaa ctcgagcacc tggatgcccg
    16621 cgtccctgag ctgctggaaa aaggccgccg gcgttccgag cgtgccgaag ctgtcccgta
    16681 tcagattgac ctggacgcca tcgcgctgcc tgtcgatcaa tgcccgggca aagcgccgcc
    16741 cgacatcgtc gtcgtccagg atgtaggtct ccatgttgat gtgatcgcgt gcgcccacga
    16801 tggccgccag catcgccgcg tacgtggctg gtccgtcctg cagcagaagc acccggttcc
    16861 ccgtggtcag cggactgccg acgatggcct gctcgcgtgc caggtggtgg tcgaagatgc
    16921 ctgtctcctc gacaccgcgg ctcagccgct gcaggatggc cttgctgcgc gaaggcgaca
    16981 ggttcccttg cgccccttgc agatgcagca cggtcgccgg cccgaggcct gggtcgggtg
    17041 cgacggctgg cgaactgctg caaccagcca ggccgagcac gcaggccgtc cagacatgcc
    17101 tataccgata ccgcggaggg gctgcgccag cgtgctggcc agcgtcggaa aggcggggca
    17161 tgagtctcct ggatgcgcag tgattgcttc ggacccagtg tcctcctcgt cgggaaacgg
    17221 ctgtgttcgg caccgcacgg aagccgaagc gcctgcggcg tagcctcctg acaagccgat
    17281 cgatgctttc tttggtcaag gctgcgggcc ctgcagcgca gaccctgcat ggacgcccca
    17341 acccccaagt cggaagagga aaagaaatga atgcaatcag gatgttgggt atcgctctga
    17401 tcctgtccgg cgcgctcggg ctggcctacg gcagcttcag ctggaccaag gaaacccacg
    17461 aggccaaggt gggccccatc gagctgtcgc tgagcgagca gcagacggtc aatgttccgg
    17521 tctgggccgg actcggcgcg atcgtggtgg gcggcgcctt gctgctgctg gctggcaagc
    17581 gcacctagca gtcaggtcgc ttcgcagcga gcgcgaatcc tttcgcgcga catcactgga
    17641 gaacggaaat gcgtcaactg atcatcggaa tctggctgct ttgctgcgcg gtgacctcgg
    17701 ccactgccca ggtcagcgtg ggcctcggca tcaatctgcc cggagtcagc atcggcatca
    17761 acctgcccgc ttacccggat ctcgtgcggg tgcctggtta cccggtctac tacgccccag
    17821 gcctgcaatc caatttcttc ttctacgacg gcatgtactg ggtgtacgag gacgacaact
    17881 ggtacgcaag ctcctggtac aacggcccgt gggggctggt gggcccgcag agcgttccgc
    17941 tcttcgtgct gcgcatcccg gtgcgctact accgatcgcc cccttcctac ttccacgatt
    18001 ggcgcgcgga cgctcctccc cgctggggtg agcactgggg caacgactgg gaacggcaac
    18061 gtcgaggatg ggaccagtgg aaccaccgct ccgccccgcg gccggcaccg ctgccggcct
    18121 accagcggaa gtactcggga gaccggtatc cgcagcagat cgatcgccaa cagcagcttc
    18181 gcggtcagaa ctaccgctac cagccgcgtg atactgccgt ccgtcaacac gaagggcagc
    18241 agcaacaacg acagaaggag cggcaggcgc agcgacctcc gcaacagaag caaccagctg
    18301 cgccgccacc cgcgcagcgc gaggagaagc cagcgccagc agtgagaggc acgaggccct
    18361 cggatttcga gcgcggaagc ccgccgaccc ggtcggaaaa ggctgtcgag aggccggcgc
    18421 agcgcccggc cgctcccgag cagcgcagtc cggcgaggcc gccccagcag cagcaacagc
    18481 cgccacgaac cgagcgcgcc cctgaacagc gcgagcagcg tgcacccggg cccggtcagc
    18541 gggacccttc tcgtcgcgag gagcagccgc gacaggagcc gagccgcggc cagggcccag
    18601 gacagggtca gggtcagggc aatgaccgcg aacgaggtga aggtcgcggc cgttagcccg
    18661 ccagcaccgc ctcttcgaga ggcttccagg agtcgcggcg gcctgcgatc tccgatgctc
    18721 gtcaatcgtc aggagacggc gccatcacca aacgctcgct ggcactgtcc agggtctacg
    18781 gcttgctcga gccgggaccg gtggtgctgc tcaccacgac gcacaagggc actcccgacg
    18841 tcatgacgat gtcctggcac acgatgatgg agttcgagcc gccactggtg ggctgcgtgg
    18901 tcagccggaa cgacttcagt ttcgccgcac tgaagtcgtc ccggcagtgc gccatcaaca
    18961 tcccgacggc ggacctcgca ccgcaggtcg tgggttgcgg caacaccacg gggcagcggc
    19021 tggacaagtt caaggccttc gggctgacgc cggtgcccgc caccgtggtc gcgccgccgc
    19081 tgatcgacga gtgctacgcc aacctcgagt gccgcgtggt cgacacgcgg ctgatgaacc
    19141 gatacgactt cttcgtgctc gaagtcgtca aggcctggat cgaccccgcc tgcaaatcac
    19201 cgagaacgtt gcaccaccgg ggccgagggc gcttcatggt cgccggggag acgatcaagc
    19261 tggcatcgag gatgaagtga tcggggcacc tcggcacgct cttgtgtccg gtcacatcgg
    19321 tccgacggcg ggggtgtccc ggctgctggc catggtcgcg ctcgccttgg ggctgtgcgg
    19381 ccatgcagcc gtcgtgcaag ccggggggca accctcgagt gcgagcaccg ccacgcggct
    19441 gcacgccaca cgcgccgaat tgcagccgcg gttgcgaacc agctccttcg gtgaaccgct
    19501 ggtgctgatc tcccgggaac tgcccgggcg gatggaaggc gatgcctatg ccgatgtgga
    19561 tcatccgttc gccgcggtcg gcgacgcctt ccgcaccgcg accggcatct gcgaactgct
    19621 cttcatgcac ctgaatgttc gttcctgcac gccgtctgcc ggagcaggcg gcgaccgcct
    19681 gacgctggtc gccggaccga agcgatccac cggaatatcc atgcaggaca gcatcagcta
    19741 caccatgcgc accgaggttg ccgacgccac ccacctgcgc gtgacgctga gcgccgcaca
    19801 gggaccgtgg tccacgcatg attacctgat ggtcttcgag gcggtgcctg tcgacgccgc
    19861 tcacagcttc gtgcacttcg gctattccta tgcctacggc gcgctggcga ggatggcgat
    19921 gggctcgtac ctggcgacgg ccggccgctc gaagatcggt ttcaccgtcg aaggaacgga
    19981 cgccgatggt cggccgaagt acgtccaggg cgtgcgcgcc gccatcgagc gcaacgtcat
    20041 gcgctactac ctcgcgctcc aggcacgctt cagcatcacc acgggctcgg cggaagaacg
    20101 cagccaggcg cggctgcgcg cctggttcgc cctcacggaa cgccacgcgg cgcagctgca
    20161 cgagtacgac atcgacgagt acctgcagga aaagcgtcgc gacctcgccc gcatggcgcc
    20221 cgggacgtga tggacagcat cccggcaccc gagcagcgac acggcagcgc aacgcacgtg
    20281 ctcgcactga acagcggttc ctcgtcgctg aaattcggcc tgtatcgcgt gggccccacc
    20341 cggatcgacc ggctggtcac aggggccgcc gagatgatcg gcgacgcggc cgccgtgatc
    20401 catgcagaga acacactggg ccagggcccg ctgcgcgaag ccatcccgat ggacgatcag
    20461 cgtgcagcga tcatccgcat cggccagctg ctctcggacc tcgaagcccc cgcgcccgat
    20521 gcgatcggcc accgcgtcgt gcatggcggg cctcagctgc gtgagcattg cctgatcgac
    20581 gaggcggtgc tgcaactgct cgaagcctca acggccttcg cgccgctgca cacgccggcc
    20641 gcgctcgcgc tgatccgttt cgccgcccgg catttcccgg gattgccgca ggtggcctgc
    20701 ctggacacca ccttccacgc ccgcatgccg tcggtggcga gcaccctgcc gctgcccgcg
    20761 gaacttcgtt cggcaggcat ccagcgctac ggatttcatg gcctgtcctg cgaatcgatc
    20821 gtcgggcaac tggccggtac gatgcctgat cgactgatca ttgcccacct gggaaatggc
    20881 gcgagcatca cggcggtcaa gcacggcacg tcgatcgaca cgagcatggg catgacaccc
    20941 acgggcgggc tcatcatggg cacgcgcagc ggcaatctcg acccgggggt gctcgtctac
    21001 ctgatgcgcg acatgaggct ggactgcgcg ggaatcgaga acctggtcga tcaccgcagc
    21061 ggtctgctgg gcatctccgg gatcagcggc gacatgcgct cgctgcacga ggctgcggcg
    21121 gagagcgccg atgcacgcct cgccatcgag atgttcagtt atgcggcagc caaggaaatc
    21181 ggcgccatgg gcaccgtgct gaacggggcg gacatgatcg tcttcaccgg aggcattggc
    21241 gagcacgatg ggctggtgcg tgccgccatc tgcgctcgtc tgtccggtct gggggtcgag
    21301 ctcgacgagg gccgcaatcg gctgggccgg ggctcgatcg gtcgcgctgg atcgcggtgc
    21361 gaggtgcagg tcctggcctc gcaggaggac gagcagatcg cccggcacgc ctggtcgctg
    21421 tgccgcggcg ccgcgaagtc aacaggccgg gcacacgctg gatcgggctg aacgactaaa
    21481 cgacctgacg atagttgctg cggccgtagt gccgatgcag gctcaggtcc tgctcgttgc
    21541 tccagtccac ggtcgcgtcg taggccggtg cgctcctgat cgattcgagg tcgagggcga
    21601 ccgatacgct gcgttctgcc caatgcaccc cctggatcca cggcggtgcg atcagcacct
    21661 tgtgaccgat ccaccagttg ctggtgtcga cgatcagaaa gcggatggcc catgtctcgt
    21721 catccacgag gaagcctgcg acgtgaccga tgtcgccgtc ggtggcgtgg atgccatagc
    21781 caaggatggc attgcagctg cgcaggtgcg ggtcgtcgtt gctgtggcgg gccttctcgt
    21841 cgcgcaggcc ggcttccagc tcacgttcgc gccctgcccg atcgtcttcc tgatgggcat
    21901 ggccgggtgc catggactgg gggtgcctgc catcgcccca gaggccggcc ccaccccagt
    21961 agtgcgggta gccgtagtgg cccaggacct gctcttcgtt ctgccgggat accggcttgt
    22021 cggtgtcgat gtccgggctg tttctcacct gcttgcgggt cagcgacacc ggcagcgact
    22081 gcgccagcca gtgaggctgg tgaacggaga tgggagagat cagcaccttg cggctcgaca
    22141 gccaggatcc ggtgtccacg acgaagtagc gaatggccca ggcatcgtcg tcgaagtaga
    22201 catcggtgac cggaccgatc tcgccgtcgc tggcgctgat cgcgtaggtc tcgaggtctt
    22261 tcacgttgcg caacatcgca ggcattcctt tcagtgtgcg ggccggcttg catcgggtct
    22321 gacgcgatgg cggcatgggt gagtgagcaa agtccgtgtg tatggaagcg gcacccatcg
    22381 cagttcgagt tccgcggtga agttctgtcg aagggactgt ctgtgcagtc tgcgggcgcc
    22441 gtctgttcgt tgccgtacga cgaagcgcag atgccccgtt gcttgatgag ccacccgggt
    22501 cgcgcctggc cgcgccgtgt tccccagcgc acagacccgc tgccgcggca ggcctatgtt
    22561 cgactcgacc gcgttgcacc tgcgatgcga gccttcgaac aacggaagta cgaacgatga
    22621 agaccgaaac gctgtccccc gatctgctgc gcaggctgga cgcctactgg cgggctgcga
    22681 actacctgtc ggtgggacag ctctacctct acgacaaccc gttgctgcgc gagccgctga
    22741 cgatggcgca catcaagccg ctgctggtgg gccactgggg cacggtgccc ggacagaact
    22801 tcatctatgc gcacctgaac cgcgcgatcc gcaagtacga cctgaacatg ttctacgtgt
    22861 cgggaccggg acacggcggt gcagcgctgg tggccaacgt ctacctcgag ggcacctgga
    22921 gcgaggttta tccaggcgtc acgcaggacc tggacggcat gaagaagctg ttcaagcagt
    22981 tctcgtttcc aggcggcatc tccagccacg tggcgccgac gacgccggga tcgatcaacg
    23041 aaggaggcga actggggtac tcgctcagcc acgccttcgg tgcagtcttc gacaaccccg
    23101 acctgatcgt cgcctgcgtg gtcggtgacg gcgaggcaga gaccggcccg ctggccgcct
    23161 cgtggcatgc gaacaagctg ctcgatccgg tcaacgacgg cgcggtgctg cccatactgc
    23221 acctcaacgg cttcaagatc agcaacccga cgctgctggc ccgcatcggc cgagaggaac
    23281 tggagcagta cttcctgggt tgcggatggg ctccgatctt cgtggaggga gacgaccccg
    23341 caacgatgca tcagctgatg gccgcggcga ccgatcaggc gatcgaacag atcctgcaga
    23401 tccaggacgt cgcacgcaac aagaatgtgc gcacgcgatc ccgctggccg ctcatcgtgc
    23461 tcaggtcccc caagggctgg acaggaccgc aggaagtcga tggcctgcag atcgagaaca
    23521 cgttccgttc acaccagatg ccgctgctgg tcagccccga tctgcccgga cacgtgaaac
    23581 tgctcgaggc ctggatgaag agctacaggc cgcaggaact gttcgacgaa agtggccggc
    23641 tgatgccgga actcgccgag ctggcgccgg ccggtgagcg ccgcatgggc gccaacccgc
    23701 acgccaatgg cggcctgttg ctgcgcgaac tgcaggtgcc cgatttccgc gaatacgcgt
    23761 ttcccgtcgc cgcgcccgga aactccgaag ctcatgacac gggggtgctc ggctccttcc
    23821 tgcgcgatgt gctgcggatg aaccgcaacc aacgcaactt ccgaatcttc ggacccgacg
    23881 agaccgtttc caacctgctc ggcgcagtct tcgaagcgac gagccgccag tgggatgccg
    23941 agaggctggc caacgacgag ttcctgcaga ccgacggcct ggtggtggac tcgatgctca
    24001 gcgagcacca gtgccagggc tggctcgaag gctacctgct gaccggccgg cacggcctgt
    24061 tcaacagcta cgaggcgttc atccgaatcg tcgactccat gttcagccag cacgcgaaat
    24121 ggctgaaggt gacgcgcgaa ctgccgtggc gccgcccgat cgcctcgctg aactacctgc
    24181 tcgcctcgca cgtctggcag caggaccaca acggattcac gcaccaggac ccgggcttca
    24241 tcgatcacgt ggtcagcaag aaggccgaca tcgtgcgcgt gtacctgccg ccggacgcca
    24301 actgcctgct gtcggtgacc gaccactgcc tgcgcagccg caactacgtc aacgtcgtga
    24361 ttgcgagcaa gcacgcgcgg cctcaatggc tgacgatcga cgaggccgtc gcgcactgca
    24421 ccgcaggcat cggcatgtgg acgtgggcgg gcaacgacca ggaagcgaat ccggacatcg
    24481 tgatggcgtg ctgcggcgat acgccgacac tcgagatcct ggcggccacg gccatgctgc
    24541 gcgagcacct gcccgagctg aaggtgcgcg tcgtcaacgt cgtcgacctg atgaagctgc
    24601 aaccggccca cgagcacccg cacgggctgg acgaagccga tttcgactcg ctgttcacgg
    24661 ctgaccgccc cgtcatcttt gccttccacg gctacccgtc gctggtgcat cggctgactc
    24721 acggtcgtgc caaccgcaac ctgcacgtgc acggctacca ggaggaagga accatcacca
    24781 caccgttcga catgcgggtt cagaacgagc tcgaccgctt ccatctggtg cagcacgcgg
    24841 tcgactgcct gccgcaactg ggcagccgtg gtgcccacct gaagcagatg acccgggaca
    24901 agctgatcga gcacaagcac tacatcgagc agcatggcca ggacctgccc gaaatccgca
    24961 actggaagtg aagccagctt ccgcactgat gcgccaccgc cggcttctac ctgtcacagc
    25021 acccttcatc cagcgcaaag gcaaaccaga atgtccagga tcgagcaact cgaaatgaat
    25081 gcgcgtcgcg agcaactgag cgcagacgtg aaggagctcg tcgagaggta ccgctccatc
    25141 ttcagttggg atgtgccgga ggtcgatgaa gcgctttcgg accggctgat cctgggtgcg
    25201 ttgcggctcg cgatcgatga catcgaaacg ggcttgccgg acggaccgct gcattgaatg
    25261 gcgagcacgg catcgccccg gaatcgacac cgggtgccgc gctgagcatc tacttcatcc
    25321 ggcacgccga aaccgcgtgg tcgctgtccg ggcagcacac aggccgcacc gacctgcccc
    25381 tgacgccgag gggcgaatcg atggcgtgcc atctggcgcc agcgctgaag gacatcccct
    25441 tctccctggt gctgaccagc ccgcggctgc gggcacgaac gacctgtcga cttgctgggc
    25501 tcgcaggttc gggaagcagc gctgcccgcg ccgagccgat gctggccgag tgggattacg
    25561 gcgacttcga gggcctgtgc accgacgaga tccggcaact gcagcctggg tgggatatct
    25621 ggcgggacgg ctgcccggga ggcgaactgc ccgccgaaac cggcgctcgc gccgacgcgc
    25681 tgctggagca gctgtgcggc ctcaccggca acgtggcgct cttctctcac ggccagttcg
    25741 ggcgggtgct ggccgcccgc tggatcgggt tgccggtctt cgaggggcgc catttcgcca
    25801 tcgatccggc gtcgatcggc atcctcggat tcgagaatgc tcaccccgag cggcgggtca
    25861 tctcgatgtg gaacgccagg gccgctgatc agccggtgcc cgtcgatgca cgagcaactg
    25921 caccggtcat cgacgcgcgg atcgactgaa gtcggggccg ctcgcagcaa tgcctgcgtc
    25981 ggtcatcgac tcgtgggtcc tggttgctat cgggccgcgg tcttctcgcg gatgcgcgac
    26041 agcatcgcgt gccatgaatc ggcgaagctg tcgacgcctt cgcgctgcag cctctgggcc
    26101 agcgcatcgt cgtccacgcc ctcgcgcctg aattcctcga gcacggcgtc ggcataccct
    26161 ccatcggccg gcatcaccgc gccgacctgg ccatgatccg cgaaggccag cagcagcatt
    26221 tccggcagcg tgatgacggt gtcgggggcc gccagggact gcacgtacaa ggtgtcgggc
    26281 gctgaagcat ccttggtcgc ggtgctcgcc cagaggagtc gctggggccg ggcgcctgct
    26341 gctgcaagac ggcgccatcg atccgaggcc agcagttcgc ggtaggcgcg gtaggttcgc
    26401 atcgcgatgg cgacgccgag ccggttgtga aacggcgggg agatctcttc cttgacgccc
    26461 gcatcccatc ggctgacaaa cagcgatgca accgaggcga ctccgaggtc gagacgggca
    26521 gccaggcgcc tttccagggc acgcatgtag gcgtgcgccg cggcgaggta gtgctcggtc
    26581 gagaacagga gcgtcacatt gaccggaacc ccgtcaaaga tggcctcctc gaccgcgctg
    26641 acgccctccg cggtacccgg gatcttgatg aacaggttcg gccgcgccgc acgctcgtgg
    26701 agcgccgacg cggcctggat ggtgcgcgcg gtgttgtgcg caagcagcgg cgagacttcg
    26761 agcgagaccc agccctccgc gccctcgctg gcgtcgaaga tcggccggaa aagatctgcg
    26821 gcctgtgtca catcttcgat ggccagttcg aagaacagct cctcgatcgc catgcccttt
    26881 gcatcgagcg cgcgtatgga ggcatcgtag gcatcgcctg ccagggccag cccgaagatc
    26941 gtcgggttcg acgtcaggcc cgtgatggac agttcggcga tgtatctctg aagcgtgccg
    27001 ctgcgcaaca gcggccggct gatgtgatcc agccacagtt gctgtcccag gtcgttcaac
    27061 tggcgggtgg gttcattcat ggggagtact ccgccttcct cgttcaagcc gttgcaacga
    27121 atcggtccgc acgagccgcg gccagcacgt caggcaggtt tgcttcgagc cgttcgatcg
    27181 ggctgtggcc ctcgagccag ctgtttggct gcacgaacca gatcgccatg gcccagccat
    27241 cgaaggcatc gccgagttcg gcgatgacgc gccgaggagc ggtagcaagg gacggcccgg
    27301 acttgtcgag ctggaacatc ggcagccacc agtcgtggtg ccactggaag cgaaacagct
    27361 ggtggtcgtc gaccagctcg tcgatgcggc gagcacggtc agacaccgag gccccgccga
    27421 cagccagaca gtgtgatcgc gagatgcccc cgtgcggtcg gtaagcggac tgcagggcaa
    27481 tgaaggcacg atcccggggc gatgggtcga caccgccgaa ttcggaacct gcgccgggca
    27541 gagcgcgtcc cccggcggat gcctgaaccg tgcggctgcc gacacccgtc agcgtgtcat
    27601 gcatgacggt gccgaaatgc gtgaacgtgg gctgaaagtg gtgtgcatgc gggctctcct
    27661 tgcccgagca tccgacacct cgcaggatcc cgtctgttcg atgccgcaca ccgcaacgcg
    27721 tcagtctgcg atggttccgg ttactaccgc atggaggtca ggccactgcg ccgaacagtg
    27781 ctccggcgcc ggcagtgacg gccatggcga cggcgcccca gaacgcgaca cgccatgtcc
    27841 cggtccacac gcgcgctccc ccgagctgcg ccgagacggc gccgagaccg gccaggaagc
    27901 ccagcgacgc cagcgtgacc cagacgatca ggtgttgcgg tgccgccgtg accaccacgc
    27961 cgatgggcag caccgcgccg acagcgaagc tcgccgccga agcaagcgcc gcctgaatcg
    28021 gacgtgcggc ggtggtctcc gagagtccca gctcgtcgcg cgcatgtgcg ccgagtgcat
    28081 cgtgggccat caactcggct gctacctggt ctgcgagcgg ccgatcgagt ccgcggctga
    28141 cgtagatccc ggccagctcc ctgcgctcgc cgacggggtc cttcgtcagc tcggcctgct
    28201 cccgtgcgag gtctgcgctt tcggtgtctg cctgggagtg gaccgaaacg aactcacccg
    28261 ccgccatcga catggctccg gcgatcaagc ccgagacact ggtcatcagg atcgtccggt
    28321 ggtcggcgcc cgcggccgcc acgcccagca ccaggctggc cgtcgaaacg atgccatcgt
    28381 tcgcaccgag gacggcggcg cgcaaccagc cgatgcggtc actgcgatgg ttctcgccat
    28441 gacgccccgc cgcttttgtg aagctgcgct ttccgtgatc agcgcggtgc gaatgggttg
    28501 tgtcgagcgt catgcgggtt ctcctttcgt agtgaccgga cgcctggcgc acgccagccg
    28561 cagagaggcc cacagggcgg gcttgtctgc agctctgcaa ccgcgttgtg ccgtcttggt
    28621 cagagggcca gggcctcccg ggtcagggcc caggcctgcc cgagcttgac gccgcgagcg
    28681 tgatacacgc tcttcacatg cttgacccgg gcttcgatct tcgcctggac ctcacccctg
    28741 gactggctca gctgcgcctt gatcacatcg cccttggctt cggcttcctg cttcagcatt
    28801 tcgacacgct gcttcgcacg atgcaccagg ccgtccagac ggctcttcgc atgcgtgagc
    28861 gtggactgca atttttcctt ggccgcgcca ctggcatatc cggcctcggc ctcgagctcc
    28921 tcgatttctg accggaacgc cgagatgtcg tggtcgaact gagcttcggc cacttcggtg
    28981 cgtgtgcgcc tgaacacgcg ccccccggcc gcctcgattg ccgcatcgac tggattgacc
    29041 cactcctcct cgacctcggc caccagggcc accttgccag gccggagcga cttctctgtc
    29101 tcctcgatga agtccaggcc cacacccgcc acccagaaat cacggaccgc tccggcgacg
    29161 gcccctgtca ccgcaccgac ggcgacgccg atcggaccac caagcagccc gatcaggctg
    29221 ccgaccgcga gacccgcacc cgttccggtg gcaccctcat tcatgctctt cttgacgctg
    29281 accgcaccct tgtcatcctt cgcgatgacg ccggcggcat agagcgtgat gtcgccgtcg
    29341 gcgtgcagct tgcgcagcgc ttgcaggccg gcttccgcag ccgtttcgtt gtcgaagatc
    29401 gataccaaca tcttgttcat ttcacatcca gttctgcttg accaaagaaa cacacgcgcg
    29461 gctgggttca gctggcggcc gtgtcaatcg acttccgaag acgcgccgtg gcggcactga
    29521 caccggcctc gaattccttc cagcgagccg cggtggcctg cttcatctca gtgagcttcg
    29581 cctcggccga cttcacatcg acctggagtg ctgagatctg cagatcgatg cgggccttgg
    29641 catcggtggc ggcggcgccg gccttcttct cgagctctga caccttcgcc ttgcccttcg
    29701 cgatgtcgcg gtctgccgcg gccacgaagc ggtcacggct cgcactgttg tctgcgtagg
    29761 tgaattcagg caacgacttg atcagttcct tggtcgcacc gggcatcacg agcttcccgc
    29821 cacggtcctg gatctgcgtg acgggaatcg ccacgtcatg acgaccgata ccgatgaagc
    29881 cgccggcgcc gacgatgacg tacgacacgt tcttgtcggg cgcgatgatc agatcctcga
    29941 tctggccgac cttctctccg gtctcgttgt ggatggtctt gcccatcagc gtcttcttga
    30001 cgctccagcc catggcgatc tgcgtcgatt cgatgatgct ggtgtcgatg gtggtggtcc
    30061 cgccagccac ctgggcccag agcggtgccg gagcacagaa cgtggcggca actgccagca
    30121 tggccagagc ctgactgcga atgttcttct tcatgatctg gttccttgtg tgttgatgcc
    30181 accagggaat tggtggcaga gacctgtcag ctcgggttgt agggttcgtg ctgcgccccg
    30241 tctgttcgac agcgcacgcg aatggccgag tccctcgggc cgcgcacgct caatacacgc
    30301 gctcaaaggc ttggcgaatt catcgtcgtt tcttcgggct caacgggctg ctcgacccga
    30361 ctgccgcccc agcgcggaag cactcgcctc atggctcttg tcagcacccc cagcgctgag
    30421 ccgctgatgc ctccgcgctc acgcacgggc cgcgcgattc ggtcggtctt cgatgcggca
    30481 ggcggtggaa gcatgggtca gtgtgggctt gcaagccgac ctggttccgt tcggtgtcgc
    30541 acccaattcc cactttcgcc tctgcctcgc tggcctcgat gtgcctgagc ctgccagaca
    30601 gggcgatgag cgagcacgcg aatccgaggc gcgtgcgctg acgcacagac catcctgcct
    30661 cgacagagca ccgtcaatgc cctcccccac cattcggaaa gaccacatga agaccgactc
    30721 gcaacttcag caggacgtga cggcagaact caagtgggag ccctcggtcc acgcggcgcg
    30781 catcggtgtc gaagtccagg atggcgtcgt gacgctttcc ggtcaggtcg attcgtatgc
    30841 ggagaagtgg agcgcagagc gtgctgcgca acgtgtttcc ggagtcaatg ccatggctgt
    30901 cgagttgaag gtggagctca cgggcttgag caagcgcacc gatgcagaca tcgggcgcgc
    30961 ggtcgagaac gtcctggaat ggacttcgtc gttgcccccc ggcgccgtca aggtgatggt
    31021 cgaaggcggg tgggtcacgc tcaccggaaa cgtggactgg aagtaccaga aacagaccgc
    31081 tgcggacagc gtccgcaatc tcatgggcgt gacgggggtg agcgatcaga tagccgtcag
    31141 accgccggtt tcctcgatcg cggtcatgtc agacatcgag tccgccctga cccgtgccgc
    31201 gatcgcggac gccgggaaga tatccgtcgc ggtgcatgga tgcgatgtga cgctgtcagg
    31261 cacggtgcac aactgggccg aacgggagac cgcgaccaac tccgcatggg gcacgcctgg
    31321 cgtgcgcaac gtcgtcgaca tgatgacgct cgcgcgttga gcgttcatcc gtccatcgcg
    31381 gctggaaagt tccggcggca tcggcgatgc cacttgacat gtcactgcac ccgaatggat
    31441 actgtacata taaacagtat ccagaaaggt cagagcgatg gacggcaggc accgacagtt
    31501 ctatcaaccc aagcccgtgc cacggattcc ggtgtgggtt cggcgtctga ttcagtggct
    31561 gtgaggtcga tggatgcctc ccgcaggcac gatctctgag ttcatgtgtt ggatttcaaa
    31621 tgattcgtat cctattgata tgaattgaaa ttcagatcct ttttacacct gatttgtcac
    31681 cagagagaaa ccatgccgag acaaacgaga aaggcggcca cctggccgcc tttgcacgtg
    31741 cgaatggtcg atttcagcca gtcgcctgtc tgattgccac ctcgaccctc cgtgccttgg
    31801 ggtcttctcc ggacacattg gcttcggcag agcgaggtct ttcgagcacg gcccggtcgg
    31861 tgccgatgcc gcgcgccttc agcaggtcga gcactgccaa agcccgctgc ttggccagtt
    31921 cctggttctg cttcacgttg cccgctgccg aatggaagcc cgatatcacc gccttcgcag
    31981 aactgcttcc ggacagtcct tgagtcagca cttccagcga tttcgcagcc tcctcagtca
    32041 gcgcggagga accgacagca aagaacaccg tcgccgattc agctgttgca ttcgcagcga
    32101 ctggcgcaga cgcaccgagt gccggcctct gtgctgcaag ttcggggtga tccttgagca
    32161 tgctcacgcc atacatcggc ttgaacgcac cctggtggca ggtggagcag ttggctttgg
    32221 caacgtctcc agttgggccc aaccggttcg gtggaaagag tttctcgatc ggctccatgt
    32281 gctgcgtgtt cagatcgcga accatccgga tgccgtacca ggccgtcact cgttgaggtg
    32341 ggctttctgc ccagttggcg aatgaccggc tgttgtggca gtaggtgcaa ttcacgccga
    32401 gggatgtcga catgtgcgtc atcaggctgt acgtccattc ggcctgcttg atggagtggc
    32461 gatttccggt gggcagcgcc gtcgttccat tgactcggat gtcttcgctg cccagcagca
    32521 agggcgtgaa cgggtcgact gggagcgacg acttgttggc gaccaccgac acggtgttct
    32581 gtccggcctt gtcgccgatc aatgcgtcgg tacgagcctc gtgcggagcc ttgaaccaca
    32641 cgttctgcgg gatgttgttg ccccggtggc aggtgtagca ggtcactccg gtggccgcga
    32701 cgtgtgattt gtagtcgctg ttgatcttgc gcgtcatctg caacatcttt cgcgcgactt
    32761 gcttggtgta gttctcgtcc gaagcaaagt tctgcgcgtt gtggcagtag gtgcaaccct
    32821 gttcaggcgc aacccatgcc gtcatcgcca ccatgagccg ggagaactca ccgaccgtca
    32881 ggtcgttgag cacctgcacg ttctggtaga tctgcgcagc aggagggccg cccggcgtgg
    32941 ccgcggctat gggtgccgga acctcgttga gcttggcctg tgtagccagt gtgcgcgggt
    33001 tatagatctc ggccatgccc gtgccgcggt agccgtgctg caccacctcg atcggaggtc
    33061 gctcgcagcc cgacaggagc gcggctacgg acagcagcac cagattcagc aggatcgtgc
    33121 tgagcttgtt catggcttga ctccctggag cgtggcggga tcgatcacag gggcccagac
    33181 gggcgggtac atcggtgcga cgtggtgctt gacggcccac aggtaccagt tgtccaccac
    33241 cgtgcctgtg agcaggatgc cgatgccacc ggtcagcggc gtcagcacgg cgaaccacca
    33301 ggcccaacgg tggatggatt ccatcgtggc gttgaagccc atcgtccagc gccagaacag
    33361 cgctgcgcgc tccgaagccg ttccgcggtc ggtgatctgc tcgatctcgc gctcaccgcc
    33421 gaacctgctg acagccagga tggtcgcgcc gtgcatggcg aacagcagcg tcgatccata
    33481 gaggaacgca atcgacagca tgtggaacgg gttgtagaaa aggtttccgt atcggatcga
    33541 gaacgccgcg gtccagtcca gatgcggaaa gatgccgaac ggcacggcct cgccccagct
    33601 gcccatcagg atcggccgga tgaagcccag caccaggaac agccagatgg ctgcgagaaa
    33661 ggcccatgcg acgtgcgtac ccagcccgag ttggcgtgcg cggcggtagg ttcgcgcgaa
    33721 ccacaggaat accgaacaag tcgtcaggaa tcctgcaagc agccaccagc ccccctggtt
    33781 catcgactgc gggatggcca gtccgttctg cggcggtgga ggttccaggc ccagccagaa
    33841 caactggcga acgaactgga tcgggttcca gtcgaccgag gccagcatgt tgaagccgat
    33901 gatgttgaac gccagcatcc ccagcatcag cgaagccacg ccgaggctgc ccagatagac
    33961 aggaccgatc tgcgcgttgc cgatgcgccc gagcaggtga acgatcagcg gctcatcgcc
    34021 ggttcggggg gcgttgccat gtggcagttc gattccgtga tggatcggcc cgacggcctg
    34081 aacgctcgtg aatatgtttt gatactcagc catgagttgt ctccttgtgc cgccgcatca
    34141 gtgccagatg ggaaggttca accaccagcc ccaccactcg ggccagcccc gggtccagaa
    34201 cggaccgctg atcacgatgc acacggcgct ccagaaaaca gccgacaagg ccaggaaaag
    34261 accaaggcgg tggatgccga gcgtgccgat cgagtaaccg atggcgtccc ggaagaacgt
    34321 gtcttcatgc tcgggtgact tcaccacctc gcccttcttc gggttggtgg atgacaggac
    34381 cagcgctccg tgcagcgaca acgcgaacgt cgtcgtgaaa aagaagctga tcgccagcat
    34441 gtgtgccggg ttgtaatgga aatggagata ttgataacct gtattcgaaa cccagtcgag
    34501 gtggctcagt attccataag gaaaaccgtg accccatgcg cccatgagca ccggccgaat
    34561 aaccacaagc gtgacataag cgaatatggc gaaagagaaa gcgatcggca catggagccc
    34621 gatcccaagc tttctgcaga tctcgacttc ccgcaatgcc cacgaactga atgcgcctat
    34681 cgcacagacc gtgatcagtt gccacaaccc gccttcacgc aacggcgcaa atcccagtcc
    34741 atatttcagg tcgggtggtg cgatattgat ctgccataca ttccaggtcc cccccagtgc
    34801 cgcaccccaa agtatcagtg cggttccgag agcggcaaag aatatcgtcg tgacgccgaa
    34861 gaatccgacg tagaacggcc ccacccagaa gtcgaagagg tctcctccaa ccagggttcc
    34921 accgcgtatg cggtactttc tttcaaaact gagcgtggcc ataagcgact cctcggagcc
    34981 cccggcgata cctggttgcc aagggctgaa atctgtctgg aagacgaggc cgggaagatt
    35041 cccgggcctc gcctaccgcg ggcgcaagcc ctttgctttt atttcgcgac gggcgaggtg
    35101 accccggccg gcagcgcagc gtgctgcgct tgcgaggcct tggctcgtgc gttcggtccg
    35161 tcgatccagt tgaaggtcga cgtgctcaac agcaccaagt gaatcatcac tgccaatccg
    35221 aacaggaaaa taccttgcac gaccatcgca cgcagcgggt cgaacaatcg ccagattctc
    35281 cacatgatca atctcctaaa gtaaatgcga cataaaggtg tacacagcac cctttacccc
    35341 gcctatcaat gatttctcag cttcagctcc cggcaaccag gaacgccact gccatgtcat
    35401 cacttgcgcg aacacggcga tcagcaaaaa caagcagaaa cccactgcaa atatcgcaag
    35461 atagactttc gaatcctttt tcaatatctg ctgcaaagtg agattcgttg attcagccat
    35521 agcattcccc ttccgggatt cagttcaaag ccaaggacgc caagcccata ccaggaagtg
    35581 cgctaccacg gcaactgccg tgaatccgat aaagccttgc acccagaatc tgtgaaactc
    35641 ctgtgcctct tcatcggtca ggcctgatat ggagcctttt ctttcagcca taggatttac
    35701 tccttaagga cggccaaacg gccggtaccg cgcagagccc gcgcgatcga ggcagccgcg
    35761 gcaccacgcc acgacatact gtgtgttcag ccgttggctg aacgaattga ttgcttgcat
    35821 acgggcacga ggtgtcatgc cggttctccc tggcgcatcg cctcgcgcga ctgcaggacg
    35881 cgggctgccg tgacacgttc ctcgcctgcc cggcgtgcgt ctcgttcggc ccgatcgcga
    35941 agccgcttgg ccgccgagat ctgcaccagc acgggttcgg tctggacgta ctcgtccagc
    36001 aactcctgtg catcgagatc ccacgggctc ggcgcctgtc cgtcgacacg cgccagggtc
    36061 ggctcgacgc gatcaagatc ggtgcccagc ggcaggatgt ggaacagcgc gtcgaacagc
    36121 gcgttgcaga gttcctggac gatgtaggtc gcgcccgagt agcccatgaa aggcgtgccg
    36181 gtgtggcggc ggatgatcgg cagcgggaac gaggccggta tgaagttcgc cttcatcatc
    36241 gaacctccgc cgcattcggc gaggtagatg cgctcgttgt agctgccgta catgaccagc
    36301 ggggtcttgt ccttcaccag ctggcggacc tcttcgttgt tcgtcttggc accgggcagt
    36361 cgcgagatgg cgaactgaca gggcagtccc atctcgtctt cgagaaagtg acggatgcct
    36421 cgggtgtagg tgtcattggc caccacggcg aagctcgccg ttccgaagaa atcctgtgtc
    36481 accgagcgcc agaggtccca gatcggcttg atggtcgtgt gcttctcgcg ctcgatgaac
    36541 ggctccggat ccagccccag cagttcaccg agcttgcgca ggaacttcgt cgtgctgtgc
    36601 agtccgatcg gtgcctgcag ataggggcgg tcgagggcct cgcagagcat gcgaccgaac
    36661 tcgcggtaca tgcagatgtt cacgtcggct tcgaccagcc gcgacacatc ggccaggtgg
    36721 ctgccgagcg ggaaggtcat gttgacttcg gcgccgatgc cctggaccag ccgccggatc
    36781 tctgccagat cgctcgggca gttgaaggtg ccgtagcagg ggccgatgat gttgacgcgc
    36841 ggcttctcgc cggccggccg ctctgcataa ggtttgcgcg ccggcacctt cttcatgccg
    36901 tactcgctcc acagccagaa catcgcgcgg ttggcgcact gccactggtc ttcgtcgatc
    36961 gtgcgcggca ggaagcgctg gatgttggtg cccgacggcg tgacgccgcc accgatcatc
    37021 tcggcgatcg agccagtgac caccaccgac ggcaagtcgg ggtcgagcgc cgcatgcgcc
    37081 cgccgcatcg agccttcggt tccttcacgc ccgagctgct cttcggccag tccggtgacg
    37141 acgatcggaa gttcgtgggg tggcagcgcg tcggtgtagt gcagcaccga agtcaccggc
    37201 aggttctcgc aaccgaccgg accatcgatg acgacttgca gacccttgat ggccgtgaag
    37261 acgtacacgg cgccccagta gccacccgcg cgatcgtgat cgaggaccag catcagatca
    37321 tctcctcggc cttgcgcttg cgggcgagct tctcggcctg ccggcgctga tcggccttga
    37381 attccgggcg atccgttgga acaccgttcg agggttccca gacaccggac gagtggccct
    37441 cgccgacttc gccgaagaat tccttcatct gctcgaagcg tgccttgttc cctatggctg
    37501 cattgatgac cgttgcgagc gaacctgcac cggccgggcc catcaaggga cgggccgaga
    37561 tcaggttggt gaagtacagc gcaggaatgc tctgggcctt ggcagcctgc acgacagggg
    37621 ttgtgccgat ggccagatcg ggctggaatt cggccatcgc agccagatcc tgttccagcg
    37681 aagcgcggaa gcggacctgc acgcccttcg actccagcca ctgcaggtct gcctcgttcc
    37741 aggcggtacg cgggcaagcg cttccgacgt agcgtagatc ggcgccgctt tcgaccagca
    37801 gcctgccgac gagcaactcg gaaccctcgt agccactgag cgtgatgcgc cccttgatcg
    37861 gagtcttggc cagggcgcca cgaatggccg gcaagaagcg gttcttggcc gcatcgatat
    37921 tcgcctgcga ggtgttggtg gcctgaccga tggcctgcag ccacgcttcg gttccgtcgt
    37981 gaccgacagg cgccgatccc acgatgggcc gccccgcggc ttcgaattcg cggatgctcg
    38041 cggtgtagaa agggtggatc gcagcgaccg cagtgcagtc gagcgcggca tacagctcgc
    38101 gccactcgcg ggtcggcacg acagggccgg atgccagccc catcggttcc agcatcatgc
    38161 cgatgatcac cggatcggcc gggaagacct cgccaagcag cgcgatcgtc ggcttgtcgg
    38221 agcggccgtt gcgcggcgcc tgaaccggtc ctgcctgcac ctcgccacgg gcgtacttca
    38281 gcatcgcgcc agccagcaca tccttggctt cggcgtgagt cggaacaccg aagccaggca
    38341 cgtcgatgcc gatgatgcgt acgccgttga tctccttggg caggatctgc agcggcacgc
    38401 cgctggccgt cggaacgcac aggttgatga tgacgatggc gtcgtacttc gccgggtcgg
    38461 cctccttgtg gacagcctcg cggatgtcct cgaagagttt gccggtgacc agcgattcac
    38521 tgttgaacgg cacgtagccg acgctgcgct tcgcaccgta gaaatgcgag gtgaaggtca
    38581 ggccgtagac acagcaggcc gagcccgaga ggatcgtcgc agtgcgacgc atgcgcaggc
    38641 cgactcgcaa cgaaccgaat gccgggcaca tgctctgtgg ctggtcgtgc gggccggctt
    38701 tcgggtcttt cggatagtcc ttcgcgtact gggccagcac ctccgacttg ccggccgact
    38761 tcgccgcggc cagcaattga tcgccaccgg cgtggcatcc catgccgtcg ccctccagcg
    38821 tcgtcgacga gacacccggc gcggtgggcg gcgtggcgct cgccgagttg gctgcaacga
    38881 tcgggatggt cttggacatg gtgcggggcc ggtcagattt cgtcgtagac gacttcgagc
    38941 gttgccttct tgctctcggt tttgccgcac atgtcgaact gcgttgcagg ttcgagcacc
    39001 acgtcgcggc cgaccgatgc ggcagagaac aacccgagca gttcgtcctg cgacatcggc
    39061 ttcggacgca tcggcagcga ggtgccgacg ttctcggcga gctgggcaaa catcggaccc
    39121 cattcggagt caggtcggcc gatgatttcg tagctggcgc tcttgcggcg gatgtcgtcg
    39181 ttggccggga tcgtcgacag caccggaatg ccgacgtgct cggcaaaggc cttggcctcg
    39241 ccggtgccgt cgtcgcggtt gatcaccatg cctgccacgc cgacgttgcc gccgagcttg
    39301 cggaagtact cgaccgcact gcacacgttg ttggcgacgt aaagcgactg caggtcgttg
    39361 ctggcgacga cgatgacctt ctggcacatg tcgcgggcga tcggcaggcc gaagccgccg
    39421 cacaccacgt cgcccaggaa gtcgagcagc acgtagtcga agccccagtc gtggaagccc
    39481 agcttctcca gcgtctcgaa gccgtggatg atgccgcgcc cgccgcagcc gcggccgacc
    39541 tcggggccgc cgagctccat cgcgtagacg ccgtcgcgct tgaagcagac gtcgccgatg
    39601 gccacgctct cgccggccag cttcttcttc gagctggtct cgatgatggt cgggcaggcc
    39661 ttgccgccga acagcaggct ggtggtgtcg ctcttcgggt cgcagccgat cagcagcacc
    39721 ttcttgccct gctgggccat catgtagctg aggttggcca gcgtgaagct cttgccgatg
    39781 ccgcccttcc catagatcgc gatgatctgg gtttccttgg tcaccggtcc cgtggccact
    39841 gcaggaggtt cgacggaagc ctcgtgacgc aggtctttca tcatgatggt cactggggcg
    39901 tccctgacag ggctcagcgt cgatggagca gtactcatga acaggacctc cagtccaaaa
    39961 tcatcttcag gcatgccggg tcgctgaatg ccgtgcggta ggcggtatcg gcctgcgctg
    40021 cttcagatcg atgtgtgatc agcccgtcca gcgacaggcg gcccgactcg gccaggtcct
    40081 tcaccgccac gagatcgctg ggcagccatt gcgctgcgat gcggatgcgc gcttcgcgca
    40141 tgaaagctgg cgggaacacg aaggaaagtg gctcgtcgta gaagccggcg agcaccactt
    40201 cgccgcccgg ggccagcctt gaaatgagcg tgtcgagcag cgaggaatca ccgctgacgt
    40261 cgcagatgca gctgtagttg cggcgcgtgt cctcggcagg atcgatcacc gcataaccgg
    40321 cggctccttc gcgacgggct ggattggttt cccagaccgt gggggagctg ccggcaacga
    40381 ccgcgagacg ggcgatcatg cggcccagca ctccgtgccc gacgatcaga tcgggctgct
    40441 tcaagcccgc catcgaatgc gcgtggtagc ccgtggccgc tagggccagc agaacgccct
    40501 gctcggccag atgctcggca aacgggatcg tgcgggctcc tggcaggacg acgtgcgcgg
    40561 cggcgccgcc gaacagtcct cgcacatcgc cgaagcattt ggcgcccggc acgaagacac
    40621 gctcgccgac gctgcggccc gacatcgagc cggctttcgc gacgcgcccg acggattcgt
    40681 aacccgggac cagcgggtag cccatccccg ggaaagctgg catgcgcccg gaccagagca
    40741 ggcgctcggt gcccgtgctg atgccactcc actcgatatc gaccgtcaca tcctcatctg
    40801 tcgcttcgga gaggtcgagg cgactgagca cgagttgctc cgggcgctcg agtaccacgg
    40861 ccatcgtgtt caactccgac ctccggcgca cgacagaagc gccctatggc aacactggtg
    40921 tgggacaagc ttcaggttca tctaccgatc actccgactg tgggtgtaat gttaagctga
    40981 catttcatac tgtcaattta catttacatt gattcgccga gggtttacac gcggcccgca
    41041 cttgccacga tgacttgggt ctggagcggc atgggatttc gaagcagccg aacgtcgccg
    41101 aaaccggccg cggtcagcag ctccgtgagc tgagaggcac tgcgcgacct cccctttccc
    41161 atggccagaa gatagaagcc gaagtacgca tcgcccatcg cctcggcgcc acgcgtgcca
    41221 gccatgggct cggtcaacag caaggttccg ccgtctggca tcgcccggcg aacggctttc
    41281 aggatctgca gggcgcgggc atcgtcgtga tcgaagatca cccgcaccag gctgatcaca
    41341 tcgcatccgg cgggcaacgg gtcgctgaag aaactcccgc cagcgactgt cgtgcgaccc
    41401 gccagacctt gttcggcgaa tcgcacccgt gcacgttcgg ccactgccgg caaatcgaaa
    41461 agtacaagct gcaggtgtgg cgcgcgctgg gccacagcag aaaggaacgt gccttcgcca
    41521 ccacccacat cgagcaagcg ccggtgcttg tcgaatgaat aggcgtcgag cacctcgtcg
    41581 gacaccaagg tctgcgaagc ggacatcagg gctgaatacg gcgacacccg cccggcatcc
    41641 agtgaaccgg gcgcatcggc tgccgcgtag ggccagtaac ttgcaagggc ggaagtcctc
    41701 gcctcacccc gcagcagtgc cacaggatct gccagatcga cgtacaaatc ggcatggtgc
    41761 tcgaccatgg aggcgatggc cgcatctccc acgagtggcg caccgagcgg tccgagcccg
    41821 aaacgacccc gactgcgctc ctcgaccaga tccagcgaca ccgccgcacg caacagtcgc
    41881 agcgcggcat cctcggagag cccgagctgc ggcgccaaag ccgacaaggt ctggggctcc
    41941 tgcgccagca tgtcgaacaa tcgcagacgg acgcaggcca gcagcacctg cgaatacacg
    42001 aacccggcca ccagatcgaa cagcccgcgc gcacgtctct gcgctatcgg gcgtgtcaac
    42061 gggaaggccg ccgcccagcg ccggaaacgc cggcttccga ccaaaccgtc acgccagctg
    42121 aaccagcggt ctttcagtcg gtcgagcaag gacgggtccg gttccagcaa cgcctgcggc
    42181 tcggacctgc cgaacgcgct catgctgcga cgcgaaccac ttcctctgcc atcgccttgg
    42241 gaacgagccg ctcggcctcg aatcggacca gcgcacgcat ctggaccgca cccgggcacg
    42301 gtggaatggc ttcaatggcg gcagcgacca ggcgatcgaa atgatggatg gcgccctcca
    42361 gccccagttc ccgcgctgca ctggggcgac acagcgcgac atcctggccg gtgggcttgc
    42421 ccagcgaaag cggatcggaa gccacgtcgc ggatgtcgtc tgccacctgg tacgcctctc
    42481 ccagccattc gccgagcgct cgccagggtg ccgaatcggc acctgcagcc tgcgcgccag
    42541 cgatggtggc ggcggcgaac aacgaaccgg tcttggcgcg ctggtagtcc gcaagcgaca
    42601 cccgaggctc gcactcccag gcctgcccgg ccacgattcc atgcggcatg cccgtggcgc
    42661 gcccgatggt ccgcagcagc atcggcaggc gaagcggtga tttcgtggcg gcagcgccca
    42721 gcgtctggaa ggccagaacg atcaacgcgt cgccagccag caccgccagg ctttcaccaa
    42781 aggcgaaatg aacggacgcc ctgccccgac gtgtcggggc gtcgtcgaag cacggcaggt
    42841 cgtcatgaac cagcgaggcg cagtgcagca actcgatcgc ggtggccgtg gcttccgaca
    42901 acagcggatc gtcgtcgccg caggcttgag ccacggcaag gcacaactgg ggccgaatgc
    42961 gggctccccc ggggaaaacg gcatgccgta tcgcaccggc cagcttgggc gggcagccct
    43021 cttcctctcc agacgcgatg gccgcagcca gagattgttc gatgcgtgtg atgggcttca
    43081 ttccggactc cggctgtgcc tgctggcggg tggctcgact ggcaggatgt aaaccgagcc
    43141 tgtcaagata atgtgtcaac taatgtagac acacgtaaac actccgtcaa cagcacaggc
    43201 ccggtgtcgc ccccggtccg tcgaaccgaa tcaacccgaa ggctgaacct ccaggcgacc
    43261 atgggccgca gaaagcgttt ctgagagcaa ggccgtagcc gcgtagatgg cgttgatgac
    43321 gggcgtcggc atccccgtga tacggccgag ttccaccacg ctgccgacca aggcctccaa
    43381 ctccagtgca cgccccagtt ccacatcgcg cagcatcgag gtcttgtggg cgccgaccgc
    43441 tgctgccccg gccaggcgtt gctcgagcga gatcttgaaa acgaccccga gccgctcggc
    43501 cacgccctga gcctcgagca tcatgtcggc ggccaacccg cgggccagcg gatggcggca
    43561 gatcccttcc agcgtcgcgt gcgtcaacgc actgatggga ttgaagctga ggttgcccca
    43621 caacttgatc cagatctcgg cgcggatgtc cctgctgacc ggcgccttga atccggcccg
    43681 catcaaaacc tgcgaaatcg cctcgacgcg ctgggtgcgc gtgccatcga gctcacccag
    43741 cgtgaagcga ttgccctcga tcacccgaac cacacccggt tcgaccagtt cggcggccgg
    43801 gtagaccacg ctgccgatca cgcgatcggt ctcgatgtgc cgagcgatga cgccaccggg
    43861 atcgacactg gtcaagccga gtccctcgta cgggcccgcg agccgatgga agtaccacca
    43921 gggcacccca ttgatcatcg tcacgacgac cgtttccggc ccgaacaacg cgcgcagctg
    43981 cggcagcacc tgatgcacct gatgcgcctt cagtgtcagc agcaccacat cctgcaggcc
    44041 ggcatcggcc gggtcccgca ccgcgcgaac gccgtgtgcg tgcttcacga tgccgtcctc
    44101 gaggatgagt cggaagcctc gctcgttgat ggcagccagg ttctcgttgc gtgcaatgaa
    44161 ggtgacttcc tcacccgaca gcgcgagcct tgcacccagg taaccgccga tcgctccggc
    44221 accaaccacc gccatcttca tgatgtgtcc cgtcctgccc gtgggctcaa ccgagcccca
    44281 gccgctgcgc cagaccgatg cgctgcaact tgccggtggc gcctttcgga agctcatcca
    44341 tgaagaggat cttcctcggt accttgtagt cggcaaggcg ctgggaaacg aaagcctgca
    44401 gttctcgttc cgtggcagga actccctcct tcaacacgac gacagcagcc acttcctcgc
    44461 ccagcttggg atgcggcacg ccgaagcaga ccacctgggc caccgcgggg tggtccatca
    44521 ggatctcgtc gacctcgcgc gggc
//
LOCUS       DAOORV010000012        44048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_960_length_44048_cov_9.560771, whole genome shotgun sequence.
ACCESSION   DAOORV010000012 DAOORV010000000
VERSION     DAOORV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 44048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_44048_cov_9.560771"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKV56_03175"
     CDS             <1..444
                     /locus_tag="PKV56_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX66842.1"
                     /translation="AFVRELSPESRYFRFISTLQELPATLLSRFTLIDYDREMALVAT
                     VRTPSDGDSPAIERIIGVSRYITNPDRSSCEFSLVVADDFKGKGLGSRLMLSIMHFAS
                     EQGLAQIEGLVLARNTDMLKLMKGLGFSIKPYADDPDFKWATKDL"
     gene            529..1797
                     /locus_tag="PKV56_03180"
     CDS             529..1797
                     /locus_tag="PKV56_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOX66843.1"
                     /translation="MKSNTAAIAADFSCANQHALATILDASQTQSIIAARDIFDISGT
                     KLWARDLPVSQALQRKLLDRQLREPLESCLKAADGVTAHTLVEAIERLLKQPTPLTAL
                     LHAHADTLLREAAKLPVHPVAQLLLTASRSSRPAAYEHATQAMALNGALMLAQGGKPT
                     DLRIAMLSGLLHDLGEMYIAPEFGEADAERTVDVLTYQQLVVHPHVGQLLIAQLTDYP
                     AEVARAIAEHHERMDGSGYPHCLRGDQLSMHGRLLAVTESTLATLSEPAAPLSRASIA
                     LRVVPGEYDLRWVGLITQVVRQQPTATAKRTAEELQERLTKLAEVMHDASTATATLRR
                     EATSAALKDALTLTEHLLARLQGGLMTSGLWSIDPVAAPDAAEVEAVEGELRLRLRRI
                     ERAVRLRAGLLTDEDARRLDDLCRQLTLRG"
     gene            1918..2229
                     /locus_tag="PKV56_03185"
     CDS             1918..2229
                     /locus_tag="PKV56_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66844.1"
                     /translation="MNSLEQWISGITAAVCMVLLVRLCLGAARQRRFDHAVRALGWEL
                     RNRSQRLWRAPAARRAATREAQAAIDRARKGRGTSDADDGEWQGNVYTPKSFRKPRKP
                     H"
     gene            2307..3212
                     /locus_tag="PKV56_03190"
     CDS             2307..3212
                     /locus_tag="PKV56_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX66845.1"
                     /translation="MTGMKRPSEIDAGVLTVAYHESGPAEGPPVVLLHGFPYDVHAYR
                     DVAPRLAERGCRVIVPYLRGFGPTRFRSADTPRSGEQAALGADLLALLDALGVSRAVL
                     AGYDWGGRAACVVAALWPERCAGLLTLNSYNIQHISQAMQPEPPAQEHRLWYQYYFHS
                     ERGRAGLMQDRRAMCRLLWQQWSPTWVFDDATFDRSAAAFDNPDFVDVAIHSYRHRYG
                     LVAGDPAYAEIERRLAQQPAIRAPTISFDGADDGLFAPRDAAHHARHFTGPRTHRIAP
                     GAGHNLPQEAPDVFADAVLELVRER"
     gene            3430..4782
                     /locus_tag="PKV56_03195"
     CDS             3430..4782
                     /locus_tag="PKV56_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX66846.1"
                     /translation="MWRRLLLSRPLWIGIVLLAIGAGAWWAWHAYHGRTDLNEQYVFA
                     TVQRGDIQDLVTATGTVQPLDYVDVGAQVSGQLKKLHVEVGSTVQAGDLLAEIDPTVL
                     KATVDARRAGLRNQRATLAERESQRVLAELLLTRQRNLVKEDATTTESLQQAEASAQA
                     AKAQVDATRASIEQTESTLRADEANLNYARIYAPLAGTVVTVTARQGQTLNANQQAPT
                     ILRVANLSTMTVQTQVSEADVSKLRRGMEVYFTTLGSQGRRWYGALRKVEPTPTVTNN
                     VVLYNALFDVPNANQSLMTNMTAQVFFVAATAKDALLIPAAALNGGTRPGDASGRRER
                     SASEPRRGDRAASAAESPASASTSGFATETARGSRRATVRLLTAAGTLEERSVELGVS
                     NRVQTQVLSGLAEGDQVVAGLKLPPAAPRNGSQSQPGSGLQQNPIGMPPGMGGGQRSR
                     "
     gene            4809..6782
                     /locus_tag="PKV56_03200"
     CDS             4809..6782
                     /locus_tag="PKV56_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HOX66847.1"
                     /translation="MPEQPAMPPDPEGRTPLIELRGVTKTYRNGELETRVLHGIDLTI
                     HEGEFVAIMGASGSGKSTLMNILGCLDKPSTGSYFFMGRDVSALDRDELALLRRDEFG
                     FVFQSYHLIAGATAAENVEVPAMYSGMPAAERHARAAHLLGELGLSQRLHHRPNQLSG
                     GQQQRVSISRALMNGGRIILADEPTGALDSSSGADVMKLLVELSQRGHTVLLITHEKD
                     IADHADRLIEIKDGRVVADPGPAAPSPTRHRFTPSVAGRNGSNLSDMLEAAKMALRSL
                     RANLFRSVLTLLGIVIGVGSVIAMLAIGDGAKQAVIDRISAMGSNLLLVRPGAPNQRG
                     FSATATLVIGDVQAIDQVPNVLAAVPEQMSNATVRFGNYDVSTSITGTSSKFPMARQW
                     PVARGSFFSAEDEAGYAAVAVLGQTTANALFPAGQDPIGQYVLVGNLLFQVIGVMSAR
                     GASPTGADQDDTVMVPYATSSLRLSGQRYLRNVTVAVADVTRIEETQAEVEALLLDRH
                     GIVDFQIRNMASIIEATVETQNTLTILLGSIAAISLLVGGIGVMNIMLVSVTERTREI
                     GIRMATGARERNIMQQFLIEAMVISAIGGAIGVAGGLATAGVIGAFGTPVQYSLLPVL
                     LASGCAFGTGLVFGYLPARKAARLDPVVALSAE"
     gene            6797..8203
                     /locus_tag="PKV56_03205"
     CDS             6797..8203
                     /locus_tag="PKV56_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOX66848.1"
                     /translation="MNSLFRLTWRSTALRAGLVGALALSGCATPIERTRPAIELPATW
                     TEASPASNTLQRDWWRGFRSAELDGLINAAQSSSPTLAIAGERVQQAELLVRSAGATL
                     FPSVGIAGGSGAQRSDPSGASARTSKSTNVSLGVSYEVDLWGRLTAGVQGAESSLAAS
                     RYDLESARLSLTTGVANAYFQVLALRMRLVIARENLAIAERVIRIVDARYRNGAASAL
                     DVSRQRITVMTQRATIEPLEVQERQTVSALAVLLGRTPQTVQVASRSFDDLALPEVGA
                     ALPSELLVRRPDLASAEAQLAGADADVAAARAALLPSLSLSASAGVASASLLSLANPA
                     TTLGLTASLAQTLFDGGRLRNQVALGESARRQLVESYRAAIYTALKEVEDALGNAVRH
                     QGQEQSQLAIRDEAQRSLRLAELRYREGADDLTSVLDAQRTLFSSLDQLAQLRLARLT
                     AALDLYKALGGGWSVSVS"
     gene            complement(8237..8929)
                     /locus_tag="PKV56_03210"
     CDS             complement(8237..8929)
                     /locus_tag="PKV56_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HOX66849.1"
                     /translation="MLIVIPNVLSGEALQQARALLRDAPWGDGRTTAGDQSAQVKNNQ
                     QLPQDHEVTAALRQLVLEGLSRSLVFFSAALPKRIFPPLFNHYGGRANAFGNHIDSAV
                     RTITFGEAMGQQVRTDLSGTLFLNDPADYDGGELVIEGLPGQPRIKLPAGDLVLYSAT
                     TVHRVEPVTRGERLASFFWLQSMVRDDGRRQLLHDMDMNLLQLRQQQGDTEPLIALTG
                     TYHNLLRMWAEV"
     gene            complement(8964..11225)
                     /locus_tag="PKV56_03215"
     CDS             complement(8964..11225)
                     /locus_tag="PKV56_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HOX66850.1"
                     /translation="MSSTTTGRSTRSRARGAAAPLLPLGALAAGFGLALLPMSGAVRA
                     QAAATPTQVDKAAEATLPAVKVQATAEATGKAAYQAVTTTVGKGRQELRDIPQSVTVI
                     TEKLIDDKNLDTLKAALHSVPGIAFEAGEGGAIGDLVRLRGFSTRGDIYLDGLRDIAQ
                     YNRDTFNQDRIEVLRGSASMLYGRGSTGGIINQVSKQPLLINQNEVAFTAGTDRYYRL
                     TGDFNLVTGQDAALRVNVMKTDADSFRNGPSTNRRGIAPTFQWGIGTSDEFSVGAYHL
                     EYDDVPDFGFRWLAGRPVGAAADRWYGTKSDYQDDSAKQFFLSHLHRFAGGGQLKTTV
                     RDGHYKRDLWSTTAGFAAVPAASSGLATACSYPAGTVTTPITSISDSSLICRGSQTRA
                     AKDRHRFVQTDYSGRFEWLGLKHDLLAGAEYANEQTTTYSYSSTPAKSPITWADDGGT
                     ASLNDTRVRARNTHFESDTTGLYVQDTLSLTPHWKLIAGLRHDRFSGDYERVAGPLSR
                     TDTVWSRRFGVLYQPTDIVSWYASYGTSFNASGDLYQFDPTSANTAPEKSRNMEVGAK
                     WDLADGDLSLRAALFRTEKYNERNTDIDQASNAFLLSGKRHTDGVEFEAVGRVTPAFE
                     VFGGIAWMKGRIDAAGSSAASQASVGRTPGLLPKVTANVWATYRVTPNWRVGAGADGM
                     SSRKPALAETGSNRAPGYAKVDALVEYVSGPYTVRLNLKNLFDKVYADGLYRGFTVPG
                     ETRAAQLTVVATF"
     gene            complement(11366..11803)
                     /locus_tag="PKV56_03220"
     CDS             complement(11366..11803)
                     /locus_tag="PKV56_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX66851.1"
                     /translation="MAFASPFDSSRRAAGPMTDINMVPLIDVLLVLLVIFIVSAPLLT
                     HAVKLDLPKASSAPQRPMPDALTLSIDAQGALYWNGQPVSRELAAQRFVEAAAQRPQP
                     ELHLRADQNVAYRHVATTLADASRAGLTRLGFVSQPLPVSPPR"
     gene            complement(11816..12673)
                     /locus_tag="PKV56_03225"
     CDS             complement(11816..12673)
                     /locus_tag="PKV56_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX66852.1"
                     /translation="MTLDTDVATAATSALPGDNALGWAHFVAHTDAVGQALLAVLLLM
                     SMLSWAVIALKALTGALQRHRSLRFVERFWNASSLSQVGADLAARPAGSHRDPYSQLT
                     LQALQAQAHHARHGAARLAEAGSASEFVTRTLKKALDAEIMRLENGLSLLATIGATAP
                     FVGLFGTVWGVYHALLAIGASAADGPGSLAQIAGPVGEALIMTGLGLAVAIPAVMAYN
                     LYTRRNRVIASQLDAFAGELHTFVAMGQPLGSPESGTAAAPPPSTRATVDAVTAPAVA
                     ALRSGPVAA"
     gene            complement(12670..13527)
                     /locus_tag="PKV56_03230"
     CDS             complement(12670..13527)
                     /locus_tag="PKV56_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOX66853.1"
                     /translation="MFQPLAFTADRSLHRDCGVVREPALLGPSASRPASAAKDEGAEL
                     TSPQRWTVVIAIGLLHLGAAGWLLRPAMNPVLPPSRETALRVDWIAAVEPAGSPTQAP
                     AALPTPAQALVRPVRSSPVAPAALLSVSAQSPAWVSVAPPDAQAARAAADDAPTRPTE
                     ASAASHAEASSSVTNAEDEIVVPASPPAPKDLPAESVQYLVPPAPVYPRLSRRQGETG
                     RVLVRVYIDEAGLPRTVQLSASSGHARLDDAARQAVQQARFKPYAENGRPMGGWALIP
                     LTFDLEKSP"
     gene            13552..13818
                     /locus_tag="PKV56_03235"
     CDS             13552..13818
                     /locus_tag="PKV56_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66854.1"
                     /translation="MEIVIHQKLGPIITRMRTIRIHIHIETSPFLEEAAMNVDAAVPG
                     GRSRAAAVAVWREAQGRRSGAGPAPLLADQAAGAVTAAVVRNTD"
     gene            complement(13770..14369)
                     /locus_tag="PKV56_03240"
     CDS             complement(13770..14369)
                     /locus_tag="PKV56_03240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOX66855.1"
                     /translation="MSTRPRVLVISGSARSGSLNQRLAAVAAGLAEQAGAQVTAVDLR
                     ALALPIYDADLEALGMPEGARQLRRLFAEHDALLLVSPEYNGFPTPLLINALDWVTRP
                     AAEEGLPSGIASLTGTVAGLFSASPGALGGLRSLLFTRQFLQMNLGMLVVPEQFGLAL
                     AEQAFDESGALRDPKSQAGVSRVVQSVLRTTAAVTAPAA"
     gene            complement(14379..14732)
                     /locus_tag="PKV56_03245"
     CDS             complement(14379..14732)
                     /locus_tag="PKV56_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOX66856.1"
                     /translation="MPKSYAQLMKQIDVLRHQAESIKRKEVEGVIKRIREAITVYGLT
                     AADLGLSGSKPGNRHSGLKRAGGALDKTGKPVRVPAKPKFRDDAGHSWSGKGPRPGWF
                     KAALASGKTIDDLTV"
     gene            14971..16788
                     /locus_tag="PKV56_03250"
     CDS             14971..16788
                     /locus_tag="PKV56_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HOX66857.1"
                     /translation="MRLTREHFRFLSGVVQGLDVRTLWDRYLYAGGSADLRRIRSVTA
                     LLQDELGGLARRAGRPEIAALLRRRSDAGVPADSAPTLEEFSATLPTDMHSQAELLAL
                     WKEAHPEGRMGRPVIRQQRLIDRQLQALQWLESLAVVVPQPPDPVSVWLDHKLSRRLA
                     AVGVDTLDSLHQWIDLHGYHWHRTVPRVGALGAARVTRWLVEHAETLGALPARSLMPR
                     SRIDSVALEPARSVGVVPLERLKLPAGLSGERGSNRAAAHLRRTPATDDLEAVQAWLA
                     QHSPASHTWRAYRREAERFLLWAAFRRGKALSSLEAEDCAAFRDFLAAPDADWTSPRH
                     VPRWSDRWRPLEGALSPASIATALGIVRNLCNWLVRMRHLESDPWADAASTDRPALPA
                     PTSRSLSDADWGAVLRWLEVQPPGVSTDRLRVLTAFACHTGLRAAELASARTRWLRCV
                     ASLEGDSKWSLCIPAEIGRTLPRAVELQGAVASQLLDYLRSTGSLDGDLPSVVDRPLF
                     AHRSDPHRSLTTGRVYTLIKGALGRCADAVELEHPATAARLRQASTHWLRHTHARQLV
                     AAGASLSSLQHRLGHRSAVSTAAYLCNPGPNEKGGTQPD"
     gene            complement(16835..17830)
                     /locus_tag="PKV56_03255"
     CDS             complement(16835..17830)
                     /locus_tag="PKV56_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX66858.1"
                     /translation="MAKKLFEKAGLIHHPLPATAERRAEPPRPEAKPKTAPGAMMHFL
                     ASQSDAIKEAEVLKTRLEAFDGASVVRSIDPSRVIASKWANRHASAFESPEFAALREE
                     IRQAGGNVQPIKVRPLHGRDDADFEIVFGHRRHRACLDLGLPVLAMVEEVSDAELFAA
                     MDRENRQRKDLSPWEQGLMYRKALDEGLYPSLRRLAEAVGADAGNVSKALTLARLPDA
                     VIEAFPTPLVIQYNWGPALSSAVQKDPEGTMARARAIALKKLGPRQVLDALTASADRV
                     VPYNTADLTITSADGKRAARISHGADGKSAVVFGKGAVSEARREELRRLIASFLG"
     gene            complement(17830..19020)
                     /locus_tag="PKV56_03260"
     CDS             complement(17830..19020)
                     /locus_tag="PKV56_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX66859.1"
                     /translation="MEPIVIGKPKRITLADIVDQAERAVGMMSEIRSAMLSPTARKSP
                     PRFNSTQVAALCGIDKGHMAYRITKGDLPAGELNSTRSRREFSLGEARQWVREYRQSA
                     LRPPGAEAVTISIGNFKGGVSKTTTAVTLAQGLSLRGHKVLVVDTDAQGSLTTLFGIL
                     PDTEVDDGDTILPLCTGAESSIRYAIRPSYWDGIDLVAAAPLLYGAEFALPARQARDP
                     EFEFWNVLHYGLDDVRAEYDVIIIDTPPALSYTTINAFMASDGLIMPLPPNALDFASA
                     AQFWSLFSDLAGGLLTNRGLDKTFEFIHVLMSRVDSSDAASAIVRTWISSVYTDKVLP
                     VEIPKTAVTATASAEFGTVYDLSRYDGNLKTFKRAKDAYERVTELIEGSVQAVWRRQL
                     EGEA"
     gene            19728..21038
                     /locus_tag="PKV56_03265"
     CDS             19728..21038
                     /locus_tag="PKV56_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HOX66860.1"
                     /translation="MDPTSADPPLRKPVNTLAIVPTKSSKITVLARKAYNVLLYLAQE
                     QGIDQEVYRAPLQSVLRGIDFNSHAREIVKQHLRAMVSTTVEWQSPTSGEGEAWNVAG
                     LLAHAKLYKQSGENWVEWSFAPNIKHELLEPQRFARLRLDVISQLRRHAGVVLYEICS
                     RYRDVGLTARQPWPWWRPVLTGSPDSEDSIKQEYRFFKRDTLKLAIAEVNAVTDLDVE
                     LIEHRAGRFIADIQFRISKKQQATLPLGRAPQPVDLTQVARARAAGLRDEDAENLIHA
                     HGNDAMSAALDALEKRAASDFPEPLRDPHRYLRALLVSTSAAAAKAAADPMAAPDARE
                     TQVAAAAVEGWTSEWVGRRRAAVIAEFEALPAQQQSDWTDRLRLQLAERGVHPSIRRR
                     LDTHGWKHQLVLGEFIKFYKGPDWDKPGPQDLLAIAAEQSARVR"
     gene            complement(21064..22020)
                     /locus_tag="PKV56_03270"
     CDS             complement(21064..22020)
                     /locus_tag="PKV56_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOX66861.1"
                     /translation="MKRLDRWGWLLAATIVHGGAVATEPTATTAAAVQPATEAVDASG
                     RPINVSQIVERVRSARYVLLGEIHDNPRHHQLRAALLRSLLADGVPTRVVFEQMDRQH
                     NAAIAAAPRDTEAIVEAGRLDRKGWAWPLHRPLFDAALEGGAAVVAGNLGRAQASQVV
                     RGGLTQAPPDLHHLLAPAVEAAADGRTAWTAALESDLLRLVEEGHCGALPRTMLAPMA
                     LAQRSRDAALASAMSQAPAGTRVVLMAGNGHVRRDIGVPHYLAVADKLSPDDSRIVSI
                     GFLERSSEGETSVDGPYDEAWFTERVARPDPCASFSPPTAPK"
     gene            complement(22017..22808)
                     /locus_tag="PKV56_03275"
     CDS             complement(22017..22808)
                     /locus_tag="PKV56_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HOX66862.1"
                     /translation="MPRFAANLTLMYTEHRFPDRFAAAAADGFDAVECLFPYELSADD
                     LAQRLRHHGLRLVLFNLPAGDWEAGDRGLAALPGREAEFASSIATALPYALASGCRQL
                     HALAGLTPQSATEADRLAMHRTYVGNLRHAARTLAPHDITLLIEPINSQSMPGYWLNL
                     QQQAHDLMAEVDEPNLRVQMDLFHCQIMEGNLASRLRRHIDGIGHIQIAGVPGRHEPD
                     IGEINYPFLFDLLDELGYAGHVGCEYQPRAGTTAGLGWWHRARAR"
     gene            complement(22829..23899)
                     /locus_tag="PKV56_03280"
     CDS             complement(22829..23899)
                     /locus_tag="PKV56_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX66863.1"
                     /translation="MLRINITPHWELGVASDALLDTTSLLALLAAVQETGAIAQGART
                     MGMSYRHAWGQIKKAEELFGHTLLNAGRGRGSTLTPLAEKLIWADRRIAARLSPMLES
                     LASELANELERTLPRPPQALRVHASHGFAIAALMQQLTAAGLQVELRYRNSFESVAAL
                     SHGECDLAGFHVPIGEFEVPAAQRYRRWLRDDTHLLVQVAVRTQGLFAAPGNPKFVRS
                     FADLRRKDLRFVNRPEGSGTRMLTELMLAKQGISHDEVTGFDSAEFTHAAVAAFIASD
                     MADVGMGVQTAAQRFGLHFIPLVRERYFLAMNRRARDMDQMPQVIQLLQSPAYRSSVA
                     ALGGYEAADTGRVLGVREAFGV"
     gene            24035..25783
                     /locus_tag="PKV56_03285"
     CDS             24035..25783
                     /locus_tag="PKV56_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOX66864.1"
                     /translation="MNAPVIPIHLLNDPSPPRPKRTQRERQVGAEALAAVRQASGDRP
                     LRRDLLIEHLHALNDRFGQLRTDHMAALAQLLKLSQAEVYEVATFYHHFDVVRENAAG
                     GFDAPPRLTVRVCDGLSCQMAGAADLLKRLPAMLEADVRVIPSPCIGRCEQAPAVAVH
                     QHPLAHASVESVVQAVQSGATTHEPEPYTGLDAYLAVGGYQTLEACISGVRDVESVIK
                     SLESSGLRGLGGAGFPAGRKWRIVRDQPAPRLMAVNIDEGEPGTFKDRYYLERDPHRF
                     LEGMLIAAWAVGIDAIYIYLRDEYHGCRALLERELARLQASPPHGYGEQGLPKIELRR
                     GAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNFETLYWIRDILEQ
                     GDEGFASHGRHGRKGLRSFSVSGRVNKPGVHLAPAGITLQELVDEHCGGMLDGHTLYA
                     YLPGGASGGILPASLAHVPLDFDTLQPHGCFIGSAAVVVLSQHDRVTDAARNLMSFFE
                     HESCGQCTPCRTGTSKARALIERDRWDTGLLAELSQVMRDASICGLGQAAPNPIDCVI
                     KYFPHELSNAQPGACA"
     gene            25780..28623
                     /gene="fdhF"
                     /locus_tag="PKV56_03290"
     CDS             25780..28623
                     /gene="fdhF"
                     /locus_tag="PKV56_03290"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOX66865.1"
                     /translation="MNAPTTHGLLKPDVPVVEFTLDGRPVQARSDETIWQVAKREGTT
                     IPHLCHKEGLTPAGNCRACVVEVEGERTLAASCCRNVSAGMKVQTDSPRSQTARKMVI
                     ELLVSDAGGTTDRHTNSSELSQWARQLGVAESRLPVRDAHYAAPDLSHAAIAVNLDAC
                     IQCTRCLRACRDEQGNDVIGLALRGSHATITFDAGDVLGQSSCVACGECVQACPTGAL
                     MPASGAGLAPITKQVDSVCPYCGVGCQLTWNVGPNDQGEERIQFVTGRDGPANHNRLC
                     VKGRYGFDYIHNPKRLTAPLIRRPGRAKDPADIERLKRGELRMDELFREASWDEALDL
                     AASGLKKVRDSAGHGSGSALAGFGSAKGSNEEAYLFQKLVRQAMGSSHVDHCTRLCHA
                     SSVTALLEGIGSGAVSNPVADVAEAELIFLIGANPTVNHPVAASFIKNAIDRGARLVI
                     CDPRHTQLTRRATWHLQFRPDTDVALLNGMLHTIINEGLVDETFIAARVNGYDALKAS
                     VAEATPERMSEICGIPASTIREVARAYATARSSMILWGMGVSQHVHGTDNSRGLIALA
                     LITGQIGRPGTGLHPLRGQNNVQGASDAGLIPMMLPNYQRVSNAAVRAHFETIWSTPI
                     DATSGLTVVEIMHAVTDGKIRGIFVEGENPAMSDPDLDHARAALAKLEILVVQDIFAT
                     ETAMLADVILPASAQPEKWGTYTNTDRLIQIGRPALAPPGLARQDLWVIEQLARRLGV
                     AWNYWQAADGEGHVAVEAPVARVYEEMRSVMDPLAGVPWTRLIREGAVVTPAHREDEP
                     GESVVFIDHFPTADGRATVVPTTYQPGVEPVDAAYPFVLTTGRVLEHWHTGAMTRHAS
                     MLDAIAPEALAVIHPIAAARLGISDGEVVNLTTHHGSVRTRASLSGDVQMGQVFLPFA
                     FWEAAANKLTGDALDAAAKIPGFKVTAVRIDRI"
     gene            28667..29710
                     /locus_tag="PKV56_03295"
     CDS             28667..29710
                     /locus_tag="PKV56_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HOX66866.1"
                     /translation="MNSPGLATRPERAADRRVVSVRAPGRLHLGFLDPGGSLGRRYGS
                     LGLVVDGFETQVDLCGATARRVTGAVPSAQAEIDRAESHLHVLRRQTGLTQPLHLHLE
                     KVLPAHAGLGSGTQLALAIGRAFALWHGLDVPSATLARWLGRGLRSGVGIGGFEQGGL
                     LVDGGPGPDGQPAPVLSRVALPDAWRVVVVLDSQHRGLSGHEEKQAIAALPAMPRAVA
                     ADICHQVLMRVLPGAACAEFASFAAGITRVQQQLGAHFAPAQGGEAYTSTSVGRLVRW
                     IGEAAGGESSAAIGQSSWGPTGFAILPSQQQADALVEAARATPLMAPHLTLQIVGGRN
                     RGATLNDPRAATA"
     gene            29755..30672
                     /locus_tag="PKV56_03300"
     CDS             29755..30672
                     /locus_tag="PKV56_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HOX66867.1"
                     /translation="MERPYILHMFTPGRQMSPFDINMSADAGYQIVVPYSGVDIGAIS
                     ALTQDVIFSRGPTGVSRTGIFIGGRDAVLAADMLDKSVKAMVKPFVVSVMADPSGAYT
                     TAAAMIACVEAALQRSHGHGLEGQKVVVIGGAGPVGRIACVIAAQAGARVCLSNRDGL
                     VEAETAAGVTGVRFGVTLQGVSGESHDTVHASLADADVILACAAAGVQVLSAADLSHA
                     RRLKVAADVNAVPPSGIAGIGVMDNGVPLADTQAVGIGALAIGNVKYQTQHRLLVRMH
                     DSHTPLVLSFPEAFATARQFLQEQACGRA"
     gene            30689..31873
                     /locus_tag="PKV56_03305"
     CDS             30689..31873
                     /locus_tag="PKV56_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOX66868.1"
                     /translation="MTMLAVAAISARAITEAAARDGYEVVALDLFGDVDTRAASVTWS
                     ALGEPGALHIDAERLRSALAGLARNADVLGWCPGAGFEGAADLLERGAAVLPLIGNDA
                     ATMRSVRDPRSFFEALDRLGIAHPEVRFEPPEDGEALHWLVKDFQGTGGWHIRRWAAG
                     SASRLGGSPALYFQREALGRSMSLTFIANGSDVCELGCNELTIRRFGAKPHVYCGAVG
                     PVMLPIPVARQVGLILRAVVAEWGLRGLGSLDFLLEGDTVSVLEVNPRPPASMALYEL
                     RRFECATSGTLRTHGLMDAHIRACLNGDLPAICPEDADAPAVRGIEIVYALRPVEVNA
                     AVTAQLSRWPGAHDLPAPGIRCTAGDPLCSVAAVGASADAVRTRLNDSRKMLLQTLET
                     ST"
     gene            31870..32874
                     /gene="mch"
                     /locus_tag="PKV56_03310"
     CDS             31870..32874
                     /gene="mch"
                     /locus_tag="PKV56_03310"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOX66869.1"
                     /translation="MTDTLPPLADTHLSVNALTQSRVQRLLNEAESLNVKAIEDPTGV
                     CIVDAGIEARSSIAAGLLIGEICMGGLGDVTLRTGVDDGWPTWVQVRSAQPVLSCLGS
                     QYAGWSLAATKEETGGRKFFSLGSGPARALAGKEALFAELGYRDRADSGVLVLEVDRL
                     PPKIVIDKVLNDCGLKPAGLTLILTPTTSWAGGTQVVARVLEVALHKAHELGFALGNV
                     VDGLASAPLPSPSPDGVVAMGRTNDAILYGGRVHLSVRGDDAAARDLALRLPSSNSRD
                     YGRGFADIFKEAGYDFYKIDGALFAPAEVWVSNVDSGNTWHAGALNMPLLQKLWLQQA
                     "
     gene            32878..33855
                     /locus_tag="PKV56_03315"
     CDS             32878..33855
                     /locus_tag="PKV56_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOX66870.1"
                     /translation="MRVAILTDETGWHTRQLQTALRSRGAVGRCVDLADCRIDTTAAW
                     HGLAIPGYGRELPDAALVRGIAGGTFEQVTKRLGVLHALRELGVPVYNDARAIERSVD
                     KAMTSLLLHAAGIPTPPTWATESAAQAQRIAIREGAAGHALVLKPLFGSQGKGLQLVG
                     EVDGIHHPLPPLDKSYGSMAYLQRFVPPVCEPGFDWRVLVVGGRAITAMRRVSRHWIH
                     NVAQGARCEPQAMTRPLAALAERAAAALALDYAGIDIVPVRCDDNDPDESDMQVIEVN
                     GVAAWHGLQRVTDFNIAAALIDDLLDRKCAGAAARSGVGPCVAPEVRRA"
     gene            33852..34787
                     /locus_tag="PKV56_03320"
     CDS             33852..34787
                     /locus_tag="PKV56_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOX66871.1"
                     /translation="MSSDTGGAIDRARAAYLRACELDIAVRKPGNVSIDSPGHGMQAR
                     QFIDSGRASAAALFARGARVGERIEAAIAATWAVAGCNTNLGIVLLCAPVAAAVERFP
                     RAASEPEMRLAIEAVLADLDETDARAAYRAIARANPGGLGSAPREDVRAEPTVTLRAA
                     MALAAHRDRIGLLYRDGYGALFDIGVAALGDDFRLTEAQAAHRSAVVDVALPVPPTRR
                     AVLRCYLALLSHAPDSHIVRKRGAGPAQTVMATAARWAVDPRLTTADPGLELDPVFVE
                     WDESLKSEGLNPGTTADMTVASMLLALITQPRSRA"
     gene            34925..35434
                     /gene="fae"
                     /locus_tag="PKV56_03325"
     CDS             34925..35434
                     /gene="fae"
                     /locus_tag="PKV56_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOX66872.1"
                     /translation="MAKIDRLMVGESLVGDGNEVAHIDLIIGPRGSAAETAFANALVN
                     NKDGFTSLLAVVAPNLLTKPATVMFNKVTIKGAKQAVQMFGPAQRGVAMAVADSVEDG
                     TIPLDEADNLFISVGVFIHWLAEDDKKIQEYNYQAVRESIKRAVNGLPTAAEVVAKKG
                     SSPHPFAAN"
     gene            complement(35540..35965)
                     /locus_tag="PKV56_03330"
     CDS             complement(35540..35965)
                     /locus_tag="PKV56_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOX66873.1"
                     /translation="MDVIFIEGFTGQTVIGIHDSELHRPQTIVVDLHAGLPRSKACDT
                     DQIADTIDYGVVRERLQVLYREHKLTLLESFAETIADMLIIDFGAAWVRVKVVKPRKF
                     DDVAAVGVVIERSAAAQPETPRAAGVLRVIGAGMVPGGN"
     gene            36275..38314
                     /locus_tag="PKV56_03335"
     CDS             36275..38314
                     /locus_tag="PKV56_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX66874.1"
                     /translation="MPLSLQNAEHHAERILDALRRGERSVATNDIVTRSWGRCVHDYR
                     LHPDQPHEPTVIARSALQERRERIGDVIECARHEMSTLFEQLADSEAAVVLTDTDGVI
                     VHMVAAPAFAADVAPLGLRPGAVWSETAAGTNGMGTCLASASPVAVQREDHFYTQFTQ
                     LTCSAVPVFDPSGELAAVLDVTSRSTLMQQHLLVLLGMTARMIENRLIDQRFPLAHPM
                     HFHSRPEFVYTLHEGKLAVAADGRIVAANRSALFQLGVQTVDDIRSRRIDDVFQTSLG
                     ELLQRSATSSFHPVVTFRANASNRFFAVARRPAGGAMAAPFPLPMGEMPGAAVSTITT
                     GRPAMRPAASRAASPSVDATFKDDRLVAHLKTARRVIARGTPVLLCGETGSGKEVFAR
                     AIHDGSPHATGAFVAINCASLPESLIEAELFGYRAGAFTGAQRTGRRGKILQADGGTL
                     FLDEIGDMPLELQARLLRVLDERQVTPLGGEEMHDVDFQLLSASHRHLPTLVREGRFR
                     EDLYYRLAGIELELPAMRDRSDKRELIRSVLASEAGSHASISREAEQLLMAHPWPGNV
                     RQLRHVLRSAAALADGAAIGPEHLPSLVASPSVSGARASSAQVDGETRPAAAASSAAP
                     VEPKLNAIQASERMMLMQLLEQHRWNLSNVAKALDISRNTLYRKLRKLHIDISHP"
     gene            38375..38968
                     /locus_tag="PKV56_03340"
     CDS             38375..38968
                     /locus_tag="PKV56_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOX66875.1"
                     /translation="MSDGDTVPVDPTPRSRFAWCLTGSGHFLEESMAIAARLPQVDLF
                     LSAAAEEVLPIYQIRIEDLRPTFRVFRDKTASSVPVGMLYDDVYHTVIVAPATSNTVA
                     KCAFGISDSLPTNMFAQAGKLGIPGVVFACDTEPVVVTKSPHDWVTLRPRRIELDNVE
                     RLRGIDHCHVVTTPAELEAWLDARLAQLSLAWNTSSS"
     gene            38947..40413
                     /locus_tag="PKV56_03345"
     CDS             38947..40413
                     /locus_tag="PKV56_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HOX66876.1"
                     /translation="MEHVVFLTGRLAQPSLERLLSGIEALPFTWEVREIGLQVAALMT
                     ADLISRRVPAPVVTSCEDGCQRRADRLIVPGRCRGDLEALTQHYGVPVLRGPEELKDL
                     PRYFNRAAVPIDLTEYEVAIFAEIVDAHRIGVDEIVGRARALAADGANVIDLGSLPDT
                     PFAHLEDSVRALKAEGFAVSVDSLNPDELLRGGRAGADYLLSLTLDTLWVADEVPSTP
                     ILISRTPTDEDSLYQAIELMQDKGRPFLADTILDPIPFGMTAAIVRYHRLRERYPDVA
                     IMMGVGNVTELTEADTSGINAVLFGICAELNVAAVLTTQVSQHARRAVREADWARRIM
                     HAAARHQTLPKGMNDALMTVHAKHPFPDTPEEIAATAALVRDPNFRIQVSPQGLHVYN
                     RDGMRLGQGAFELWPQLKLEDDAAHAFYMGVELAHAETALQLGKRYVQDQPLDWGCAV
                     ERAPVDLAGWCAPGTTMQTGKMRGKLPADPADETSERT"
     gene            40416..40994
                     /locus_tag="PKV56_03350"
     CDS             40416..40994
                     /locus_tag="PKV56_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HOX66877.1"
                     /translation="MNDVIYETVVTTLSADGVSHVAPMGIRYRGDEVVLMPYRPSTTL
                     DNIVATGCAVLNIVVDTRVFAGSVTGRKVWDTLPAERIPGVRLACALSHLELRLVSRQ
                     DEEQRTVLHLAREHACVHAPYIGINRAQAAVVEGAVLVSRLSMLPAGKVDSEMAYLQI
                     AIDKTAGPEEREAWGWLREAVAKHRRHAQQQQ"
     gene            40991..41722
                     /locus_tag="PKV56_03355"
     CDS             40991..41722
                     /locus_tag="PKV56_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HOX66878.1"
                     /translation="MTTATMRMLVSVRDVQEALEAAEGGADFIDLKEPREGALGGLPL
                     NTIREVVCALRAHHLRLPISATIGDLPMEDVAQILTRVADVGSCGVDYVKVGIERETG
                     AAAVLDALASCGWPVVPVFIADRGLDNELVTRACNIAGGAGAAFPAIMVDTAEKQAGS
                     LFELLSPEALQRFIDAVRGAHAMVGLAGALRLAHVERLAALQPDFVGFRSAVCVGDRA
                     SQLDAGRVRDLSARLRAAVHHELHA"
     gene            complement(41736..42350)
                     /locus_tag="PKV56_03360"
     CDS             complement(41736..42350)
                     /locus_tag="PKV56_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOX66879.1"
                     /translation="MLMWVVKLGGSLCEQAPETSPLRDWLDLLVREGAGRVVIVPGGG
                     RFADAVRGAQAQWRFDDLTAHNMALLAMSQTAHLLRSLNPALRLCDREAALHQALRQG
                     AVAVWSPLELQRDRADADTNWDVTSDSIALGLALRLGAARLIVVKSCEIDPSAALDQL
                     SARGAVDRRFAMLAEGQSLRIDVVGQRTCSLMLGELKVRHDKGG"
     gene            complement(42344..43438)
                     /locus_tag="PKV56_03365"
     CDS             complement(42344..43438)
                     /locus_tag="PKV56_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOX66880.1"
                     /translation="MQRSDTFIVGWDVGGAHLKACLTRDGQVIDVAQWACPLWQGLDQ
                     IDIALQQAQARWPALGSDTRHAITMSGEMVDAFEHREEGVQRIADRLAQVLRGEVRLY
                     GGDLGWIAPADAARCWTQIASANWLATAHHAATVFRDGLLVDIGSTTTDLIVLANGRV
                     QTASRSDHERLASGELVYHGVVRTPLCALARHVAFRGPPVNVMNEWFATTADVYRMTG
                     ELDPAHDQQPSADARSKDRTATRQRLARMIGLDARDAEDDEWLALAKAWRQAQIEEIA
                     ASLTRMMATRCGTASAGTIVTAGCGAFLVPDLAARVPALTGWPLRSYGCDVARIAATD
                     DGAADASKADWAQVCAPSVAVASLYAREPC"
     gene            complement(43425..>44048)
                     /locus_tag="PKV56_03370"
     CDS             complement(43425..>44048)
                     /locus_tag="PKV56_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOX66881.1"
                     /translation="AIRLASSKRATLARLQQHSIATPLDFMTDAAVRHWVVKPDDGAG
                     SLDTRRHGDHAAALMDWQRRGQRGETATLEPWVEGDALSLSLQVHNDCAELLAVNRQR
                     LAIDGEGAVSLIEVDIGAGDFDAAQRHAFEQTARNVCAAIPGLAGWVGIDLVWHARRG
                     PVVVEVNPRVTSAYVGMSAALGRNLAAEILSAHEAAHLQTEITDAAL"
BASE COUNT         7046 a        14878 c        14950 g         7174 t
ORIGIN      
        1 gcgttcgtgc gcgaactgtc ccccgagagc cggtacttcc gcttcatctc gacgctgcag
       61 gagttgccgg ccaccctgct ttcgcgcttc acgctgatcg actacgaccg cgagatggcg
      121 ctggtcgcga cggtacgcac cccctcggac ggcgacagcc cggcgatcga acgcatcatc
      181 ggcgtgtcac gctacatcac caaccccgat cgcagcagct gcgagttctc gctggtcgtg
      241 gccgacgact tcaagggcaa gggtctgggc tcgcggctga tgctgtcgat catgcatttc
      301 gccagcgaac aagggctggc acagatcgaa ggcctggtgc ttgcgcggaa taccgacatg
      361 ctgaagctca tgaagggact gggcttcagc atcaagccgt atgccgacga tcccgatttc
      421 aagtgggcga ccaaggacct gtagccaccg gcctcgccgg cactcaagaa agccgggatt
      481 gcgacgaaag agcgggtaga gatcacccag tccgccctcg gagccgccat gaagtccaac
      541 accgccgcga tcgcagccga tttctcctgc gccaatcagc acgccctggc caccatcctg
      601 gatgccagcc agacgcagag catcatcgcc gcgcgcgaca tcttcgacat ctcgggcacc
      661 aagctgtggg cacgtgacct gccggtgtcg caggcgctgc agcgcaagct gctcgaccgc
      721 cagctgcgcg agccgctcga aagctgcctg aaggccgcgg acggcgtgac cgcacacacg
      781 ctggtcgaag ccatcgagag gctgctcaag cagcccaccc cgctgaccgc cctgctgcat
      841 gcgcacgccg acaccctgct gcgcgaggca gccaagctgc cggtgcaccc ggtggcgcag
      901 ctgctgttga cggcctcgcg aagcagtcgt ccggcggcct acgagcacgc gacacaggcg
      961 atggccctca acggcgcatt gatgctggct cagggcggca agcccaccga tttgcgcatc
     1021 gcgatgctga gcggcctgct gcacgacctg ggcgagatgt acatcgctcc ggagttcggc
     1081 gaggccgacg cagaacgcac cgtcgatgtg ctgacctacc agcaactggt cgtgcatccg
     1141 cacgtcggac agctgctgat cgcccagctg accgactacc ccgccgaggt cgcccgggcg
     1201 atcgccgaac accacgagcg catggacggc tcgggctatc cgcattgcct gcgcggcgac
     1261 cagctgtcga tgcacggccg gctgctcgcg gtgaccgagt cgacgctcgc cacactgtcc
     1321 gagccggcag cgccgctgtc gcgcgccagc atcgcactgc gcgtggtgcc cggcgagtac
     1381 gacctgcgct gggtcggtct gatcacgcag gtggttcgcc agcaaccgac cgcaacggcc
     1441 aagcgcacgg cggaggagct gcaggagcgg ctgacgaagc tcgccgaggt catgcacgac
     1501 gcgagcacgg ccaccgcgac gctgcgccgc gaggcgactt cggccgcgct gaaggacgcg
     1561 ctgacgctga ccgaacactt gctggcccgg ctgcagggtg gattgatgac cagcgggctg
     1621 tggagcatcg accccgtggc ggctcccgac gcggccgaag tcgaggcggt ggaaggcgaa
     1681 ctgcgcttgc gactgcgccg catcgagcgc gcggttcgac tgcgcgccgg cctgctcacc
     1741 gacgaggatg cccgccgtct ggacgacttg tgccgccagt tgaccctgcg cggttgaacc
     1801 cgcgggcttt cgccacctca tcgatgtggc ttgccagccg gcgcaggcgt catgccccga
     1861 tctccgcgat gatcacgatc tgaatccggc tcttgccgtg tctcaaccgg tcgcacgatg
     1921 aactccctcg aacagtggat ctccggcatc accgccgccg tgtgcatggt gctgctcgtt
     1981 cggctctgcc tcggcgcggc gcgtcagcgg cgcttcgatc acgcagtgcg cgcgctcggc
     2041 tgggagctgc gcaaccgctc acagcgcctc tggcgcgcac cggccgcgcg ccgggctgcc
     2101 acgcgcgaag cgcaagccgc catcgaccgg gcgcgcaagg gccggggcac ctccgatgcc
     2161 gatgacggcg agtggcaagg caacgtctac acgccgaagt cgttccgcaa gccacgcaag
     2221 ccgcactgac ccgcccggga cctgtatttc gggcggtaga gtcgggcgcg caacggttga
     2281 ctcttcggca cccgccctgc cccaccatga ccggaatgaa gcgcccaagc gagatcgacg
     2341 ccggcgttct gactgtcgcg taccacgaga gcggacctgc cgaaggaccg ccggtggtgc
     2401 tgctgcacgg gttcccgtac gacgtccatg cctaccgcga tgtcgctccg aggctggccg
     2461 aacggggctg tcgcgtcatc gttccgtacc tgcgaggctt cgggccgacg cgcttccggt
     2521 cggccgacac gccgcgctcc ggtgagcagg cagcgctcgg tgccgacctg ctggcgctgc
     2581 tggatgcgct gggcgtgtcg cgtgccgtgc tggcgggcta cgactggggc ggccgcgcgg
     2641 cgtgtgtcgt ggcggcactg tggcccgagc gatgtgccgg gttgctgacg ctcaattcct
     2701 acaacatcca gcacatctcg caggccatgc agcccgagcc ccctgcacag gagcaccgac
     2761 tctggtacca gtactacttc cacagcgaac gcgggcgcgc cgggctgatg caggaccgcc
     2821 gcgccatgtg ccgcctgctc tggcaacagt ggtcgccgac ctgggtgttc gacgacgcca
     2881 cgttcgatcg cagtgcggcg gcattcgaca acccggactt cgtcgacgtc gcgatccatt
     2941 cctatcgtca tcgctacggc ctggtggccg gcgacccggc gtatgccgag atcgagcggc
     3001 gcctggcgca gcagccagcg attcgcgcgc caacgatcag cttcgacggc gccgacgacg
     3061 gattgtttgc acctcgtgac gccgcgcatc acgcgcgaca cttcaccggg ccgcgcacgc
     3121 accgcattgc tccaggtgcc ggacacaacc tgccgcagga ggcccctgac gtcttcgccg
     3181 atgcggtgct ggagctggtg cgcgagcggt gaagcgccgc cgtggccgga cagcccgatc
     3241 ggccggtgaa acccggaccg tctttcggcg tcggacatcg atcacccgcc accgccggca
     3301 agcagcccct ttacacaacg gcaacacggg cccctgcatt gcttaaccac cggcgcgcga
     3361 cactcggtgc cgcgcgccct gccccgaacc tccgatccca tgcccgagac acccgccctc
     3421 ctccgcccgt tgtggcgtcg cctcctgctc agtcggcccc tctggatcgg catcgtgctg
     3481 ctggcgatcg gcgccggcgc atggtgggcc tggcacgcgt accacggccg caccgatctg
     3541 aacgagcagt acgtgtttgc caccgtccag cgtggcgaca tccaggacct ggtcaccgcc
     3601 accggcacgg tgcagccgct cgactatgtg gacgtgggcg cccaggtgtc cggtcagctg
     3661 aagaagctgc atgtcgaggt gggctcgacg gtgcaggccg gggatctgct ggccgagatc
     3721 gaccccacgg tgctgaaggc caccgtcgat gcgcgacgcg ccggcctgcg caaccagcgg
     3781 gccaccctgg cggagcgcga atcgcaacgg gtgctggcgg aactgctgct cacgcgtcag
     3841 cgcaacctgg tgaaggagga cgccaccacc accgaatcgc tgcagcaggc cgaagccagt
     3901 gcgcaagccg cgaaggccca ggtcgatgcg acgcgcgcgt ccatcgagca gaccgaatcg
     3961 acgctgcggg cagacgaggc caacctgaac tacgcccgca tctacgctcc gctggcaggc
     4021 acggtggtca cggtcaccgc ccggcagggg cagaccctga acgcgaacca gcaggcgccg
     4081 accatcctgc gcgtcgccaa cctgtcgacg atgacggtgc agacacaggt gtcggaagcc
     4141 gatgtcagca agttgcgacg cggcatggag gtctacttca cgaccctggg cagccagggt
     4201 cggcgctggt acggcgcgct gcgcaaggtc gagccgacgc cgaccgtcac caacaacgtg
     4261 gtgctctaca acgcgctgtt cgatgtaccg aacgccaacc agtcgctgat gaccaacatg
     4321 acggcacagg tgttcttcgt cgcggccacg gcgaaggacg cgctgctgat ccccgctgcc
     4381 gcattgaacg gcggcacgcg cccgggcgat gcgtcggggc gacgtgaacg ctccgccagc
     4441 gagccgcggc gcggggaccg cgctgcgtcg gcagccgaga gcccggcctc cgcatcgacc
     4501 agcggattcg cgaccgagac cgcgcgcggg tcgcgacgcg ccaccgtcag gctgctgacc
     4561 gccgcaggca ctctcgagga acgctccgtc gagctgggtg tcagcaaccg cgtccagacc
     4621 caggtcttgt cgggtctggc cgagggcgat caggtcgttg cggggctcaa gctgccaccg
     4681 gcggcaccgc gcaatggctc gcagagccag cccggcagcg gcctgcagca gaacccgatc
     4741 ggcatgccgc cgggcatggg cggcggccag cgcagtcggt gatctcagtg gaccgcatcg
     4801 acccggcgat gccggagcag ccagccatgc cgcccgatcc cgagggcagg acaccgctga
     4861 tcgagttgcg cggcgtcacc aagacgtacc gcaacggcga actcgagacg cgcgtgctgc
     4921 acggcatcga cctgacgatc cacgaaggcg agttcgtcgc gatcatgggg gcgtccggtt
     4981 cgggcaagtc gacattgatg aacatcctcg gctgcctcga caagccgagc accggcagct
     5041 acttcttcat ggggcgcgac gtgtcggcac tcgatcgcga cgagctggcg ctgctgcggc
     5101 gcgacgagtt cggcttcgtg ttccagagct accacctgat cgctggcgcc accgcggcgg
     5161 agaacgtcga agtgccggcg atgtactcgg gcatgcctgc agcggaacgc cacgcgcgcg
     5221 ccgcacacct gctcggcgag ctgggcctgt cgcaacgcct gcaccaccgg ccgaaccagc
     5281 tctcgggtgg tcagcagcag cgggtgtcga tctcgcgggc cctgatgaac ggcggtcgca
     5341 tcatcctggc cgatgagccg accggcgcgc tcgacagcag cagcggcgcc gacgtgatga
     5401 agctgctggt cgaactctcg cagcgcggcc acaccgtgct gctgatcacc cacgagaagg
     5461 acatcgcgga ccacgcggac cggctgatcg agatcaagga cggccgtgtg gttgcagatc
     5521 ctggcccggc ggcaccttcg ccgacccggc accgtttcac gccctcggtg gcggggcgca
     5581 acggatcgaa cctctcggac atgctggagg ccgcgaagat ggcactgcgc tcgctgcgcg
     5641 cgaacctctt ccgttcggtg ctgaccctgc tgggcatcgt gatcggcgtg ggctcggtga
     5701 tcgcgatgct ggccatcggc gacggcgcca agcaggcggt gatcgaccgc atcagcgcga
     5761 tgggatcgaa cctgctgctg gtgcgtccgg gtgcgcccaa ccagcgtggc ttcagcgcga
     5821 cggccacgct ggtgatcggc gacgtgcagg ccatcgacca ggtcccgaat gtgctggccg
     5881 ccgtacccga acagatgagc aatgccaccg tgcgcttcgg caattacgac gtcagcacca
     5941 gcatcaccgg cacctcttcg aagttcccga tggctcgcca atggccggtg gcgcggggca
     6001 gcttcttcag cgcggaggac gaagccggct acgcagccgt cgcggtgctc ggccagacca
     6061 cggcgaatgc gctgttcccc gccgggcagg acccgatcgg ccagtacgtg ctggtgggca
     6121 acctgctgtt ccaggtgatt ggcgtgatgt ctgcgcgagg cgcgtcgccg accggcgccg
     6181 accaggacga cacggtgatg gtgccgtatg ccaccagcag cctgcggctg tcgggtcagc
     6241 gctacctgcg caacgtgacg gtggcggtgg ccgacgtgac acgcatcgag gaaacccagg
     6301 ccgaggtcga ggcgctgctg ctggatcgcc acggcatcgt cgatttccag atccgcaaca
     6361 tggcctcgat catcgaagcc actgtcgaga cgcagaacac gctgacgatc ctgctgggct
     6421 cgattgcggc gatctcgctg ctggtgggcg gcatcggcgt gatgaacatc atgctggtgt
     6481 cggtgaccga gcgcacccgc gagatcggca tccgtatggc gaccggcgca cgcgagcgca
     6541 acatcatgca gcagttcctg atcgaggcga tggtcatctc ggccatcggc ggcgccatcg
     6601 gcgtggccgg tggactggcc acggccggcg tgatcggcgc attcggcacg ccggtccagt
     6661 attcattgct cccggtgctg ctggcctcgg gctgcgcctt cggcaccggc ctggtcttcg
     6721 gctacctgcc tgcccgcaag gcggcgcggc tcgacccggt cgtcgccctt tccgcggaat
     6781 gacacgatcc tcgcccatga actctctttt ccgattgacc tggcgcagca cggcactgcg
     6841 ggccggcctc gtcggcgccc tggcgctgtc cggttgcgca acgccgatcg agcgcacgcg
     6901 acccgccatc gagctgcccg ccacctggac cgaggcctcg cccgccagca acaccctgca
     6961 gcgcgactgg tggcgcggct tccggtcggc cgaactggat ggcctgatca acgcagcaca
     7021 gtcaagcagc ccgacgctgg ccatcgccgg tgagcgtgtg cagcaggccg agctgctggt
     7081 gcgcagcgcc ggagccacgc tgttcccctc ggtcggcatc gcgggcggca gcggcgccca
     7141 gcgcagcgac ccgtcaggcg ccagcgcacg aaccagcaag tccacgaatg tgtcgctggg
     7201 cgtcagttac gaggtcgatc tgtggggccg gctcactgcc ggcgttcagg gcgccgagtc
     7261 ctcgctggcc gcgagccggt acgacctcga atcggcccgc ctctcgctga cgaccggcgt
     7321 ggccaacgcg tatttccagg tgctggcgct gcgcatgcgg ctggtcattg cacgcgagaa
     7381 cctggccatc gccgagcgcg tgatcaggat cgtcgatgca cgctaccgca acggcgcggc
     7441 ttcggcgctc gacgtgagcc ggcagcgcat caccgtgatg acgcagcgcg cgacgatcga
     7501 gccgctggag gtgcaggagc ggcagaccgt ttcggcactc gcggttttgc tcggccgcac
     7561 accgcagacc gtgcaggtcg cgtcccgcag cttcgacgac ctggcccttc ccgaagtcgg
     7621 tgccgcgctg ccgtccgagc tgctggtgcg ccgtcccgac ctggcgagtg ccgaagcgca
     7681 gctcgccggt gccgatgccg atgtggcggc agcgcgcgcc gcgctgctgc ccagcttgtc
     7741 gctgtcggca tcggccggcg tggccagcgc ttcgttgctg tcgctcgcca acccggcaac
     7801 cactttaggc ctgacggcct cgctcgcgca gacgttgttc gacggcggcc ggctgcgcaa
     7861 tcaggtggcg ctcggtgaat cggcacgccg gcaactcgtc gagagctacc gtgccgccat
     7921 ctacacggcg ctgaaggaag tcgaagatgc cctcgggaac gcggtgcgcc atcagggaca
     7981 ggaacaatca cagctcgcga tccgcgacga agcgcagcgc tcgctgcgac tggccgagct
     8041 gcgctatcgc gaaggcgccg acgacctgac cagcgtgctc gatgcgcagc gcaccctgtt
     8101 ctcgtcgctg gaccagctcg cccagctgcg gctggcgcgg ctgaccgcgg cgctggatct
     8161 ttacaaggcg ctgggcggag gctggtctgt ctccgtctca tagcggatct gcaggttcag
     8221 accgcgcaac cggcggtcac acctcggccc acatccgcag caggttgtga tacgtcccgg
     8281 tcagcgcgat cagcggctcg gtgtcgccct gctgctggcg cagctgcagc aggttcatgt
     8341 ccatgtcgtg cagcagctgg cgccgcccat cgtcacgcac catgctctgc agccagaaga
     8401 agctggccag ccgttcgcca cgcgtgacgg gctcgacacg gtggacggtc gtcgccgagt
     8461 acagcaccag gtcgccggcc ggcagcttga tgcgtggctg gccgggcagg ccctcgatga
     8521 ccagttcgcc gccgtcgtag tcggcggggt cgttgaggaa cagcgtgccc gacaggtcgg
     8581 tgcgcacctg ctggcccatc gcctcgccga aggtgatggt gcgcaccgcg ctgtcgatgt
     8641 gattgccgaa cgcgttggcg cggccgccat agtggttgaa cagcggcggg aagatgcgtt
     8701 tcggcagcgc cgcactgaag aacaccaggc tgcggctcaa gccctcgagc acgagctgtc
     8761 tcaacgccgc cgtcacctcg tggtcctggg gcagctgctg gttgttcttg acctgtgccg
     8821 actggtctcc ggctgtcgtg cggccatcac cccagggggc atcgcgcagc agtgcacggg
     8881 cctgctgcag cgcttcgcct gacagcacgt tcggaatcac gatcagcatg ggatgtcctt
     8941 tcaggtggcg gtcgcacggt gaatcagaac gtcgccacca ccgtcagctg cgccgcgcgc
     9001 gtctctcccg gcacggtgaa gccgcggtac aggccgtcgg cgtacacctt gtcgaacagg
     9061 ttcttcaagt tcagccgcac ggtgtacggg ccgctcacgt actcgaccag cgcatcgacc
     9121 ttcgcgtaac ccggcgcgcg gttgctgccg gtctcggcca gcgccggctt gcggctgctc
     9181 atgccgtcgg ctccggcacc gacgcgccag ttcggtgtga cgcggtaggt ggcccagacg
     9241 ttggcggtca ccttcggcag caggcccggc gtcctgccga ccgaggcctg cgatgccgcc
     9301 gaactgccgg ccgcgtcgat gcgccccttc atccacgcga tgccaccgaa cacctcgaac
     9361 gccggcgtga cgcgccccac cgcctcgaac tcgacgccgt cggtgtggcg cttgccgctc
     9421 agcaggaagg cgttgctggc ctggtcgatg tcggtgttgc gctcgttgta tttctcggtg
     9481 cggaacagtg cggcgcgcag cgacaggtcg ccgtccgcca ggtcccactt cgcgccgacc
     9541 tccatgttgc ggctcttctc gggtgcggtg ttcgcgctgg tcgggtcgaa ctggtacagg
     9601 tcgcccgacg cgttgaacga ggtgccgtac gaggcgtacc agctgacgat gtcggtcggc
     9661 tggtacagca cgccgaagcg gcggctccag acagtgtcag tgcgcgacag cggcccggcg
     9721 acgcgctcgt agtcgcccga gaaacggtcg tggcgcaggc cggcaatcag cttccagtgc
     9781 ggcgtcagcg acagcgtgtc ctgcacatac aagcccgtgg tgtcggattc gaaatgcgtg
     9841 ttgcgcgccc gcacccgcgt gtcgttcagt gacgcagtgc cgccgtcgtc agcccaggtg
     9901 atcgggctct tcgccggcgt gctgctgtag ctgtaggtgg tggtctgctc gttcgcgtat
     9961 tcggcaccgg ccagcaggtc gtgcttgagc ccgagccatt cgaatcggcc gctgtagtcg
    10021 gtctggacga agcgatggcg gtccttcgcg gcgcgcgtct ggctgccgcg gcagatcagg
    10081 ctgctgtcgc tgatcgaggt gatcggcgtg gtcacggtgc cggccgggta gctgcatgcg
    10141 gtggccagcc cgctcgatgc cgccggcacc gccgcgaatc cggcggtggt agaccagagg
    10201 tcgcgcttgt aatggccgtc gcgcaccgtg gtcttcaact ggcctccgcc ggcgaatcgg
    10261 tgcaggtgac tcaggaagaa ctgcttcgcc gagtcgtcct ggtagtcgga cttcgttcca
    10321 taccagcggt cggcagccgc accgaccggc cgcccggcga gccagcggaa accgaagtcg
    10381 ggcacatcgt cgtattcgag gtggtacgca ccgacgctga actcatcgga cgtgccgatg
    10441 ccccactgga aggtcggcgc gatgccgcgg cggttcgtcg acggtccgtt gcggaagctg
    10501 tcggcgtcgg tcttcatgac gttgacgcgc agcgccgcat cctgtccggt gacgaggttg
    10561 aagtcaccgg tgagccggta gtagcggtcg gtgccggcgg tgaacgcgac ctcgttctgg
    10621 ttgatcagca gcggctgctt gctgacctgg ttgatgatgc cgccggtcga gccgcggccg
    10681 tacagcatcg acgccgagcc gcgcagcacc tcgatgcggt cctggttgaa ggtatcgcgg
    10741 ttgtactgcg cgatgtcgcg caggccatcg aggtagatgt cgccgcgggt cgagaagccg
    10801 cgcagccgca ccaggtcgcc gatcgccccg ccctcccccg cctcgaacgc gatgccaggc
    10861 acgctgtgca gcgcggcttt cagcgtgtcg aggttcttgt cgtcgatcag cttctcggtg
    10921 atcaccgtca ccgattgcgg gatgtcgcgc aactcctgcc gccccttgcc gaccgttgtt
    10981 gtcaccgcct ggtaggccgc cttgccggtg gcctcggccg tggcctgcac cttcacggcc
    11041 ggcaacgtgg cctcggcagc cttgtcgacc tgggttggcg tggcagcggc ctgtgcacgc
    11101 acggcgcctg acatcggcag caacgcgagt ccgaagcccg cggccagggc gccgagcggc
    11161 agcaacggtg cagcggcacc gcgtgcgcgg gaccgcgtcg agcggccggt ggtggtggaa
    11221 gacatcggag atctcctgag gtgggttctg aaactcggtc ccgggtcggc aggcgtcgtc
    11281 agaccaagcg cggcccgcac ccgcgcgcag cgggcgttta ttcgcggggt cgtgggtgtc
    11341 taggtccgca gcgcgtgctg ccgcgtcatc ggggtggcga caccggcagc ggctggctga
    11401 cgaagcccag tcgggtgagt ccggcgcgcg atgcatcggc cagcgtcgtg gccacatggc
    11461 ggtaggccac gttctgatcg gcacgcaggt gcaactcggg ctgcggccgc tgcgcggcgg
    11521 cctcgacgaa gcgttgcgca gccagttcgc gggacaccgg ctggccgttc cagtacagcg
    11581 cgccctgcgc atcgatggac agcgtcagcg cgtcgggcat cggccgttgc ggcgccgaac
    11641 tggctttcgg caggtcgagc ttgacggcgt gcgtcagcag cggcgcgctg acgatgaaga
    11701 tcaccagcag caccagcagc acgtcgatca gcggcaccat gttgatgtcg gtcatcgggc
    11761 cggcggcacg ccgcgaggag tcgaaggggg atgcgaaggc catgtgattc gctcctcagg
    11821 cagcgaccgg tccgctgcgc agggcggcga ccgcgggggc tgtgacggcg tccaccgtcg
    11881 cgcgtgtcga cgggggcggt gccgcagcgg tcccggattc cgggcttccc agtggctgac
    11941 ccatcgcgac gaaggtgtgc aactcgccgg cgaacgcatc gagttgcgat gcgatcaccc
    12001 ggttgcgccg cgtgtacagg ttgtaggcca tcaccgcggg gatcgcgacc gcgagcccga
    12061 gcccggtcat gatcagcgcc tcgccgaccg gcccggcgat ctgggccagc gaaccggggc
    12121 cgtcggccgc gctcgcaccg atcgccagca gcgcgtgata gacgccccag acggtgccga
    12181 acaggccgac gaacggcgcg gtggcaccga tggtggccag cagcgacagg ccgttctcga
    12241 gccgcatgat ttccgcatcg agcgccttct tcagcgtgcg ggtgacgaac tcgctggcgg
    12301 atccggcttc ggcaagacgc gcagcgccat ggcgtgcatg gtgcgcctgc gcctgcaggg
    12361 cctgcagggt cagttgcgag tacggatcgc ggtggctgcc ggccggccgg gccgccagat
    12421 cggccccgac ctgcgacagc gagctggcgt tccagaatcg ctcgacaaag cgcaggctgc
    12481 gatggcgctg cagcgcaccg gtcagcgcct tcagcgcgat gaccgcccag gacagcatcg
    12541 acatcagcag cagcacagcc agcagcgcct ggccgactgc atcggtgtgc gccacgaagt
    12601 gagcccagcc aagcgcgttg tcgcccggca gggccgaagt ggcagcagtt gccacgtccg
    12661 tgtcgagggt catggtgatt tctccaggtc gaaggtcaag ggaatgagcg cccagccgcc
    12721 catcgggcgc ccgttttccg cgtagggctt gaagcgcgcc tgctgcacgg cctgccgcgc
    12781 cgcatcgtcg aggcgggcgt ggcccgacga cgcactgagc tgcaccgttc gtggcagtcc
    12841 ggcctcgtcg atgtagacac gcaccagcac gcgtccggtc tcgccctggc ggcgcgacag
    12901 gcgcggatag accggcgccg gtggcacgag gtactgcacc gattcggccg gcaggtcttt
    12961 cggcgccggc ggcgatgcag gcaccacgat ttcgtcctcg gcgttcgtga ccgacgagga
    13021 cgcctctgcg tgcgatgccg ccgacgcctc ggtgggacgg gtgggagcgt cgtcagccgc
    13081 ggcgcgggcg gcctgcgcat cgggcggcgc cacgctgacc cacgcaggcg attgcgcgga
    13141 aaccgacagc aacgccgcag gagccacggg gctcgaccgg accggtcgaa cgagcgcctg
    13201 cgctggcgtc ggcaaggcag caggagcctg tgtgggagac ccggcaggtt cgaccgcggc
    13261 gatccagtcg acccgcagcg cggtctcgcg tgatggcggc agtaccgggt tcatcgccgg
    13321 tcgaagcagc cagcccgccg cccccaggtg cagcagtccg atcgcgatca ccaccgtcca
    13381 gcgctgcggc gacgtcagct cggcgccttc gtccttcgcg gccgatgcgg ggcgcgacgc
    13441 ggacggaccc agcagcgctg gctcgcgcac gacgccacag tcgcggtgaa gcgaacgatc
    13501 cgcagtgaag gcaagaggct ggaacatgtg cagcgaagtc gaaacggtcc gttggaaatc
    13561 gtgatccacc agaaactggg accgatcata acgcgcatgc gaacgattcg cattcatata
    13621 cacatcgaaa cttcaccttt tcttgaagaa gccgcgatga atgtcgatgc tgccgtgccg
    13681 ggaggacgca gccgcgcggc ggccgtcgcg gtgtggcgcg aggcccaggg ccgccgatcc
    13741 ggtgcgggac ctgccccgct gctcgcagat caggccgcgg gcgcggtgac agctgctgtc
    13801 gttcgcaaca ccgactgaac cacccggctc acccccgcct gggacttcgg gtcgcgcaat
    13861 gcaccggact cgtcgaaggc ctgctcagcc agcgccagcc cgaactgctc gggcacgacc
    13921 agcatgccga gattcatctg caggaactgg cgggtgaaca gcaggctgcg caggccgccc
    13981 aacgctccgg gtgacgcgct gaacagaccg gccaccgtac cggtcaggga cgcgatgcct
    14041 gaaggcagac cctcctcggc ggcaggacgc gtgacccagt cgagcgcgtt gatcagcagc
    14101 ggcgtcggaa acccgttgta ctcgggcgaa accagcagca gggcgtcgtg ctcggcgaac
    14161 agacggcgca actgccgcgc gccctccggc atgccaagcg cttcgagatc ggcatcgtag
    14221 atcggcagcg caagcgcccg cagatccacc gccgtcacct gagcgccggc ttgctcggcc
    14281 aggcccgcgg cgacagcggc aagtcgctga ttgagcgagc cgctgcgggc gctgccactg
    14341 atcaccagaa cgcgtggtcg ggtactcatg gaagccgatc agaccgtcag gtcgtcgatg
    14401 gtcttgccgc tggccagtgc cgccttgaac cagcctgggc gcggcccctt gccggaccac
    14461 gagtggccgg cgtcgtcgcg gaacttcggc ttggccggca ccctgaccgg cttgccggtc
    14521 ttgtccagcg caccacccgc ccgcttcagc ccggaatgcc gatttcccgg cttcgaaccg
    14581 gacaggccca gatcggcggc ggtgagcccg tacaccgtga tcgcttccct gatgcgcttg
    14641 atgacgccct ccacttcctt tcgcttgatg ctttcagcct gatgcctgag cacatcgatc
    14701 tgcttcatga gttgggcgta agacttgggc atgggaacgt ctccaggctt gaaggaatga
    14761 gtcgagccgc tcgcgattgt gcgtctggcg gaggaaacct cgctcggcca gccgtcgctg
    14821 acctcacggc acgtgcccct atcgaaacgg gaggcacgcg tcagatagac atagctagct
    14881 tgataatttt aattatcatt gcattcactt tatgctgact tctgcccgac tcagccgacg
    14941 caagcccctg ccggcgctcg tgaagccggt atgcgactga cgcgcgagca cttccggttc
    15001 ctgagcggtg tcgttcaggg gctggacgtg cgaacgctct gggaccggta cctctacgcc
    15061 gggggctcag cggatctccg ccgcatccgc tcggtgacag cgctgctcca ggatgaactc
    15121 ggtgggttgg cccgacgcgc cggccgaccc gaaatcgccg ccctgctgcg cagaaggtcg
    15181 gacgccggcg tgcccgccga cagcgcgccg acgctggagg agttttctgc cactctgccc
    15241 acggacatgc acagtcaggc cgagttgctc gccctgtgga aggaagccca ccccgaaggg
    15301 cgaatgggtc ggccggtgat tcgacagcag cggctgatcg atcgccagct ccaagccttg
    15361 cagtggctgg agtcgctggc ggtcgtggtg cctcaaccgc cggatccggt gtccgtctgg
    15421 ctcgaccaca agctgtcacg ccgcctggcc gcggtgggcg tcgacacgct ggacagcctg
    15481 caccagtgga tcgaccttca cggctaccac tggcaccgca ccgtgccccg cgtgggcgca
    15541 ctgggggcag cccgcgtcac gcgctggctg gtcgagcatg ccgagaccct gggcgccctg
    15601 cccgctcgct cgctgatgcc gcgctcacgc atcgattcgg ttgcactcga accggcgaga
    15661 tctgtcggtg tggttccgct ggagcgattg aagctcccgg cggggctgtc gggcgagcgg
    15721 ggatccaacc gggcggcggc gcacctgcgc cggaccccgg ccaccgacga cctggaagcg
    15781 gtgcaggcat ggctcgctca gcattcaccc gcatctcaca cgtggcgcgc ctaccggcgt
    15841 gaggccgaac gctttctgct ttgggcggct tttcggcgag gcaaggccct gagttcgctc
    15901 gaggccgaag actgcgcggc ttttcgcgac ttcctggctg cccccgacgc cgactggacg
    15961 agcccgcgtc atgtgcctcg ctggtccgat cgatggcggc ccctggaggg cgcgctgtcg
    16021 cctgcatcga ttgccacggc gctgggcatc gtcaggaacc tctgcaactg gctggtccgg
    16081 atgcgccacc tggaatcaga cccctgggcc gatgccgcct cgacggaccg ccccgccttg
    16141 ccggcaccga catcgcgctc gctgagcgac gcggactggg gggctgtgct gcggtggctg
    16201 gaggtgcagc ctccaggcgt ttccaccgat cgcctgcgcg tgctgacggc cttcgcgtgt
    16261 cacaccgggc tgcgtgccgc ggagctggcc agcgcgcgaa cgcgctggct gcgctgcgtt
    16321 gccagcctcg agggcgactc gaagtggtcg ttgtgcatcc cggcggaaat cgggcggacc
    16381 ttgccgcgcg ccgtcgagct tcagggcgcg gtcgccagcc agttgctgga ttacctgcgg
    16441 agcacaggca gtctcgatgg ggatctcccc agcgtggtgg accggcctct gttcgcacat
    16501 cgatcggatc cgcacaggtc gctgacaacc ggccgggtct acaccctgat caagggtgcg
    16561 ctgggtcgct gcgccgacgc cgtggagctt gagcatccgg cgacggcggc acgattgcgg
    16621 caggcttcga cccactggct gcgccacacc catgccaggc agctggtggc cgctggcgcg
    16681 tcgctgagct cactgcagca ccggcttggc catcgatcgg ccgtctcgac ggccgcctac
    16741 ctctgcaatc cgggcccgaa cgagaagggc ggtacgcagc ccgactgacc gttgtaccgt
    16801 acaacacccg gcgcagcggc cgcgccgccg tcggtcaacc gaggaaactg gcgatcaaac
    16861 gccggagttc ctcgcgccga gcctcggaga cggcgccctt gccgaaaacc accgccgact
    16921 tgccgtcggc gccatggctg atgcgcgccg cgcgcttgcc atcggccgac gtgattgtca
    16981 ggtcggcggt gttgtacggt acaacgcgat ccgccgaagc ggtgagggcg tccagcacct
    17041 ggcgcgggcc gagcttcttc agcgcaatgg cccgggcacg tgccatggtt ccttcgggat
    17101 ccttctgcac cgccgacgac agcgccggcc cccagttgta ctgaatcacc aacggtgtgg
    17161 gaaaggcctc gatcaccgca tcgggcagac gcgccagggt caacgccttg ctgacattac
    17221 cggcgtctgc gccgaccgcc tcggccagcc gcctcagcga cggatacagg ccctcgtcca
    17281 gcgccttgcg atacatcaac ccctgttccc aggggctgag atccttgcgc tgccggttct
    17341 cgcgatccat cgccgcgaac agttcggcgt cggagacctc ctcgaccatg gcaagcacgg
    17401 gcaagccgag gtcaaggcag gcgcgatgcc gccggtggcc aaagacaatc tcgaagtcgg
    17461 catcgtcgcg cccgtgcaga ggtcgcacct tgatcggctg gacattaccg ccggcctggc
    17521 ggatttcctc ccggagcgca gcgaattcgg gcgactcgaa cgcggatgca tgccggttgg
    17581 cccacttcga ggcgatcacg cgagacggat cgatggagcg caccaccgac gctccgtcga
    17641 aggcctcgag tcgcgtcttc aggacctcgg cctccttgat cgcgtccgac tgggatgcca
    17701 ggaaatgcat catcgcgccg ggcgcagtct tcggcttggc ctcaggccgg ggcggctcgg
    17761 ctcgcctttc cgcggtggcc ggcagcgggt ggtggatgag gccagccttc tcgaaaagct
    17821 tctttgccat cacgcttctc cttccaactg gcggcgccag accgcctgca ccgagccctc
    17881 gatcaactcc gtcacgcgct cgtaggcgtc cttggcacgc ttgaatgtct tcaggttccc
    17941 gtcgtatcgg ctcagatcgt agacggtgcc gaattcggcc gatgcggtgg ccgtgacagc
    18001 cgtcttcggt atctcgaccg gcagcacctt gtcggtgtag accgagctga tccaggtgcg
    18061 cacgatggcg ctcgccgcat ccgacgaatc aacccgcgac atcagcacgt ggatgaactc
    18121 gaacgtcttg tcgaggcccc ggttggtcag cagcccgccg gccaggtcgg agaacaggct
    18181 ccagaactgc gccgcagacg cgaagtcgag cgcgttgggc ggcagcggca tgatcaggcc
    18241 gtcgctcgcc atgaacgcgt tgatcgtcgt gtacgacagc gccggtggcg tgtcgatgat
    18301 gatcacgtcg tattcggcgc gtacatcgtc cagcccgtaa tgcagcacgt tccagaactc
    18361 gaactccggg tcgcgcgcct gcctggccgg cagcgcaaac tccgcaccgt agagcagggg
    18421 ggctgcggcg acgagatcga tgccgtccca gtagctggga cgaatcgcgt agcgaatcga
    18481 gctttccgcg ccggtacaca gcggcaggat cgtgtcgcca tcgtccacct cggtgtcggg
    18541 caggatgccg aagagtgtgg tcaggctgcc ctgggcatcg gtgtcgacca ccagcacctt
    18601 gtggccgcgc aggctgaggc cctgggccag cgtcaccgcg gtggtcgtct tgctgacacc
    18661 acccttgaag ttgccgatcg agatcgtgac cgcctcggcg ccaggcgggc gcaatgcaga
    18721 ttggcggtac tcgcgcaccc actgccgcgc ttcaccgagg ctgaactcgc gccgcgaacg
    18781 ggtcgagttc aattcaccag ccggcagatc gcccttggtg atccgatagg ccatgtgccc
    18841 cttgtcgatg ccgcacaacg cagccacctg ggtgctgttg aaacgcgggg gcgacttgcg
    18901 agcggtggga gacagcatcg cgctgcggat ttcgctcatc atgccgacag cgcgttcggc
    18961 ttgatcgacg atgtctgcca gggtgatgcg tttcggtttt ccgatgacga tgggttccaa
    19021 cacgatctcc agaacagtcg acaaacaagc gagtcaacgc gattgcgcca actgtcgcca
    19081 ttgtgtcaaa tcccggggag gccgcgtacg ttttccacgc gagatcctcg caaccacgca
    19141 tcaagccggt atttgaccgc agctcggcca ggagcgggat cgaaaaccag gccggtgcgg
    19201 gttttcaatt tcattctctt caagctcttt aaatacttca ggctgcgaaa accccatgcc
    19261 ggtcaaagac ttaggcgatt ccgagtgagt gcatacaggt tgatgcgtga gggactttag
    19321 gcggtgaggt gcggggatgt gttcgcctgc gtgcaggaaa ctgggctgac aggtgagtgc
    19381 atgtaggcca gctcgtcacg gaagcgattg ggcggtgcgg tgttggtgct ctttcagttc
    19441 acgcgtcggc ccatcggctt tcaggtgagt gaaaataggc gcgggcaggc aaagatctcg
    19501 ctcggagccg ccttcctggc ggttcttgca tgatgggcgg cacaccggga tgcgcagagg
    19561 atcggtgaat tgatgacgtg agtcgattta ggcgggtatc cggcttggtc ccacggcagc
    19621 tctcatccca gacgtgagac gttctaggac cctggccgcg ccaggtgatc tgttccgatc
    19681 acatttccat tgcggatgaa tctggttcac gtcaaactcg atcaatgatg gatccgacgt
    19741 ccgccgaccc ccctttgcgc aagccggtca acacgctggc gatcgtgccg accaagtcca
    19801 gcaagatcac ggtgctggcg cgcaaggcct acaacgtgct gctttacctc gcacaggagc
    19861 agggcatcga tcaggaggtc tatcgcgcgc cgctccaaag cgtgttgcgc ggcatcgatt
    19921 tcaattcgca tgcccgggag atcgtcaagc agcatctgcg cgcgatggtc agcaccaccg
    19981 tcgaatggca gtcgccgact tccggggaag gcgaggcctg gaacgttgcg ggcctgctgg
    20041 cgcatgccaa gctctacaag cagtcgggcg agaactgggt cgaatggtcg ttcgcgccca
    20101 acatcaagca cgaactgctg gagccgcagc gcttcgcgcg cctgcggctc gacgtcatct
    20161 cgcagctgcg gcggcatgcc ggtgtcgtgc tctacgagat ctgctcacgc tatcgcgacg
    20221 tgggtctgac cgcgcgccag ccctggccct ggtggcggcc ggtcctgacc ggcagcccgg
    20281 acagcgagga ttcgataaag caggagtacc ggttcttcaa gcgcgacacg ctgaagctgg
    20341 ccatagcgga ggtcaacgcc gtgaccgatc tcgatgtcga gctgatcgag caccgtgccg
    20401 gccgcttcat cgcggacatc cagttcagga tcagcaagaa gcagcaagcc acgttgccgc
    20461 tgggccgggc accccagccc gtcgacctga cccaggtggc gcgagcgcgc gcggccgggc
    20521 tccgggacga ggacgccgaa aacctgatcc atgcccacgg caacgacgcg atgagtgcgg
    20581 cgctcgatgc cctcgagaag cgcgctgcca gcgacttccc tgagccgctg cgcgacccgc
    20641 atcgctatct gcgcgcgctg ctcgtgtcga cctcggcggc ggcggccaag gccgcagcag
    20701 atccgatggc tgcgcccgac gcgcgcgaga cgcaggtcgc cgccgcagcg gtcgagggct
    20761 ggacctctga atgggtcggc cgacggcgtg cggcagtgat cgccgagttc gaggccttgc
    20821 ccgcgcagca gcagtccgac tggacagacc ggctgcggct gcagcttgcc gaacgtgggg
    20881 tgcacccgtc gatccgccgg cgactcgaca cccatggctg gaagcaccag ctggtgctcg
    20941 gtgaattcat caagttctac aagggcccgg actgggacaa gccggggccg caagacctgc
    21001 tggccatagc cgcagagcag agcgcacgcg tgcgctgacc gcgtcgtact cgctgcgcgc
    21061 gcgctatttc ggcgccgtcg gcgggctgaa gctggcgcac ggatcggggc gcgccacgcg
    21121 ttccgtgaac cacgcctcgt cgtaggggcc atcgaccgag gtttcgccct cgctcgagcg
    21181 ttccaggaag ccgatgctga cgatgcgcga atcgtctggc gagagcttgt ccgccactgc
    21241 gagatagtgc ggtaccccga tgtcccgtcg cacgtggccg ttgccggcca tgaggacgac
    21301 gcgggttcct gcaggcgcct gggacatggc cgacgccaac gctgcatcgc ggctgcgctg
    21361 cgccagcgcc atcggagcca acatggtgcg gggcagggcg ccgcagtgac cctcttcgac
    21421 gaggcgcagc agatcggact cgagagccgc tgtccaggcc gtccggccat cggcagcggc
    21481 ctctaccgcc ggtgccagga ggtgatgcag atcgggtggc gcctgcgtca ggccgccgcg
    21541 gaccacctga ctggcttgcg cgcgacccag attgcccgcg accacggctg cccctccttc
    21601 cagcgctgcg tcgaacaagg gacggtgcag tggccacgcc cagcctttgc ggtccagtcg
    21661 tcccgcctcg acgatcgcct cggtgtcgcg gggcgcggcc gcgatggcag cgttgtgctg
    21721 tcggtccatc tgctcgaaga ccacacgggt cggcacgccg tcggccagca gggatcgcaa
    21781 cagcgcggcg cgcagctggt ggtgtcgtgg gttgtcgtgg atctcgccga gcagcacgta
    21841 gcgcgcgctg cgcacccgct cgacgatctg actgacgttg atcgggcgcc cggaggcgtc
    21901 gactgcctcg gtggcgggct gaacggctgc cgcggtggtc gcggtcggct cggtcgccac
    21961 cgcgccgccg tggacgatgg tggccgccag caaccagccc cagcgatcca gccgcttcac
    22021 cgcgcgcgtg cccggtgcca ccagccgagg ccggccgtgg tgcccgcccg aggctggtac
    22081 tcgcagccga cgtggccggc atagcccagc tcgtccagca gatcgaacag gaacgggtag
    22141 ttgatttcgc cgatgtccgg ctcgtggcga ccgggtacgc cggcaatctg gatgtgtccg
    22201 atgccgtcga tgtgccgcct cagccggctt gccaggttgc cttccatgat ctggcagtgg
    22261 aacaggtcca tctgcacccg caggttcggc tcatccacct cggccatcag gtcgtgcgcc
    22321 tgctgctgca ggttcagcca gtagccgggc atgctctgcg agttgatcgg ttcgatcagc
    22381 agcgtgatgt cgtgaggcgc gagcgttcga gccgcgtggc gcaggttgcc gacgtaggtg
    22441 cggtgcatcg ccagccggtc tgcctcggtc gccgactggg gggtcaggcc ggccagcgca
    22501 tgcagttgcc gacagcccga ggcgagcgcg tagggcaggg cggtggcgat ggacgaggcg
    22561 aactccgcct cgcgtcccgg cagcgcggcc agaccccggt cgccggcttc ccaatcgccg
    22621 gccggaaggt tgaacagcac cagccgcagg ccgtgatggc gcaggcgctg cgccagatcg
    22681 tcggcgctga gttcgtaggg aaacaggcac tcgaccgcgt cgaatccgtc tgcggcggcg
    22741 gcggcgaaac ggtccgggaa tcgatgctcg gtgtacatca gcgtcaggtt ggcggcgaat
    22801 cgcggcatgg caaggtgagg ccgtggtgtc agacgccgaa ggcctcgcgc acgccgagta
    22861 cgcggccggt gtcagcggcc tcgtagccgc cgagcgcggc gaccgacgag cggtaggctg
    22921 gtgactgcag cagctggatc acctgaggca tctggtccat gtcgcgggca cgccggttca
    22981 tcgccaggaa gtagcgctcg cgcaccagcg ggatgaaatg caggccgaaa cgctgcgccg
    23041 cggtctgcac gcccatgccg acatcggcca tgtcgctggc gatgaacgca gcaaccgcgg
    23101 cgtgcgtgaa ctcggcgctg tcgaagccgg tgacctcatc gtgcgaaatg ccctgcttgg
    23161 ccagcatcag ctcggtcagc atgcgggtgc ccgagccctc ggggcggttg acgaaacgca
    23221 ggtccttgcg tctcaggtcg gcgaagctgc gcacgaattt cgggttgcct ggtgcggcga
    23281 acagcccctg cgtgcgcacc gccacctgca ccagcagatg ggtgtcgtcg cgcagccagc
    23341 ggcggtagcg ttgtgcggcg ggcacctcga attcgccgat gggcacgtgg aagccggcca
    23401 gatcgcactc gccgtgcgag agcgcagcca ccgactcgaa gctgttgcga tagcgcagtt
    23461 ccacctgcag gccggcggcg gtcagctgct gcatcagtgc ggcgatcgcg aagccgtggc
    23521 tggcatgcac ccgcaaggcc tgcggcggtc tcggcagggt gcgctccagc tcattcgcga
    23581 gctcggacgc cagcgactcg agcatgggcg acaaacgggc ggcgatgcgg cgatcggccc
    23641 atatcagctt ttcggccagc ggcgtcagcg tggagccgcg cccgcgcccc gcgttcagga
    23701 gcgtgtggcc gaacaactcc tcggccttct tgatctggcc ccaggcatgc cggtagctca
    23761 tgcccatggt gcgggcaccc tgggcgatcg cgccggtttc ctgcaccgcc gccagcaatg
    23821 ccagcaggga ggtcgtgtcc agcagcgcat cggacgcaac gcccagttcc cagtgcgggg
    23881 tgatgttgat tcgcagcatt aagcaatcgt tttcatatcc tgaaggcgcc gcagttatat
    23941 cgtcagcagg taattcagtc cagtcgaata gttttggcaa aacatatacg cgctgtatgt
    24001 tccgccgacc gtgccttgcg aaagatcgct gcccatgaac gcccccgtca tccccattca
    24061 tctgctgaac gatccctccc cgccgcgccc gaagcggacc cagcgagaaa ggcaggttgg
    24121 cgccgaggcg ctcgccgcgg tcaggcaggc gagcggagac cggcctctcc ggcgcgatct
    24181 gttgatcgag catctgcatg cgctgaacga tcgattcggt cagctgcgca ccgatcacat
    24241 ggcggcgctg gcgcaattgc tgaagctgtc gcaggccgag gtctacgagg tcgcgacctt
    24301 ctatcaccac ttcgacgtgg tccgcgaaaa cgcggcaggc ggcttcgatg cgccgccgcg
    24361 cctgacggtg cgtgtctgcg atggcctgtc atgccagatg gccggagccg ccgatctgct
    24421 gaagcgtctg cctgccatgc ttgaagccga cgtgcgcgtg atcccgtcgc cgtgcatcgg
    24481 gcgctgcgaa caggcgccgg cggtggccgt gcatcagcat ccgttggcgc acgccagcgt
    24541 cgaatcggtg gtgcaggccg ttcaatcggg ggcgaccacg catgaacccg agccttacac
    24601 cggactcgat gcctaccttg ccgtgggcgg ctaccagacg ctcgaggcct gcatcagcgg
    24661 agtccgcgat gtcgagtcgg tgatcaagtc gctcgagagc tcgggcttgc gaggtctcgg
    24721 tggcgccggg ttcccggccg gccgcaaatg gcggatcgtt cgcgatcagc cggcaccgcg
    24781 cctgatggcc gtcaacatcg acgagggtga gcccggcacg ttcaaggatc ggtactacct
    24841 cgaacgcgat ccgcaccgat tcctcgaagg catgctcatc gctgcctggg cggtcggcat
    24901 cgatgcgatc tacatctacc tgcgcgacga gtaccacggt tgccgcgcgc tgctcgagcg
    24961 cgaactggcc cgcctgcagg ccagcccacc ccacggttac ggcgagcagg gcttgccgaa
    25021 gatcgagttg cgtcgtggcg ccggcgccta catctgcggt gaagagtccg cgatgatcga
    25081 atcgatcgaa ggcaagcgcg gcatgccgcg tctgcgccca ccgtatgtgg cgcaggtcgg
    25141 cctgttcggt cggccgacgc tcgagcacaa cttcgagacc ctgtactgga tccgcgacat
    25201 cctggagcag ggcgacgagg ggttcgccag ccacggccgc cacggccgca aggggctgcg
    25261 ctcgttctcg gtgtccggac gggtcaacaa gcccggcgtc cacctcgctc ccgcgggcat
    25321 cacgctgcag gaactggtcg acgaacactg cggcggcatg ctggacggcc acacgctcta
    25381 cgcctacctg cccggcggtg cttcgggcgg catcctgccg gccagcctgg cccacgtacc
    25441 actcgacttc gacaccctgc aaccgcacgg ctgcttcatc ggttctgcgg cggtggtggt
    25501 gctttcgcag cacgaccgcg tgaccgacgc ggcgcgcaac ctgatgtcgt tcttcgaaca
    25561 cgaaagctgc ggccagtgca caccgtgtcg caccggcacg tcgaaggcgc gtgccctgat
    25621 cgagcgcgac cggtgggaca ccggcctgct ggccgagctg tcgcaggtga tgcgcgacgc
    25681 gtcgatctgc gggctggggc aggcggcgcc gaatccgatc gattgcgtga tcaagtactt
    25741 cccgcacgag ctgtccaacg cacagccagg agcctgcgca tgaacgcccc cacaacacat
    25801 ggcctgctga agcccgacgt gccggtggtc gagttcacgc tggacggccg gcccgtgcag
    25861 gcgcgcagcg acgaaaccat ctggcaggtc gcgaagcgcg aaggcacgac gattccgcac
    25921 ctgtgccaca aggaaggcct gacgccggcg ggtaactgcc gcgcctgtgt tgtcgaggtc
    25981 gagggcgagc gaaccctggc ggcgtcatgc tgccgcaacg tcagcgcggg gatgaaggtg
    26041 cagaccgaca gcccgcgttc gcagactgct cgcaagatgg tgatcgaact gctggtcagc
    26101 gacgccggcg gcaccaccga ccggcacacg aacagctccg aactgagcca gtgggcacgg
    26161 cagctcggtg tggccgaaag caggttgccg gtccgcgacg cccattacgc cgcgcctgac
    26221 ctgagtcacg cggccatcgc ggtgaacctc gacgcctgca tccagtgcac ccggtgcctg
    26281 cgcgcctgtc gcgacgaaca gggcaacgat gtcatcggct tggcgctgcg tggttcgcac
    26341 gccaccatca cgttcgacgc cggcgatgtg ctcggccagt cgagttgcgt cgcctgtggc
    26401 gaatgcgtgc aggcctgccc gaccggcgcg ctgatgccgg cgagcggtgc gggcctggcg
    26461 ccaatcacca agcaggtcga ttcggtctgc ccgtactgcg gggtcggctg ccagctcacc
    26521 tggaacgttg gcccgaacga ccagggcgaa gaacgcatcc agttcgtcac cggccgcgac
    26581 ggcccggcca accacaaccg gctgtgcgtc aaggggcgct acggcttcga ctacatccac
    26641 aacccgaagc ggttgactgc cccgttgatc cgccgtccgg gtcgcgccaa ggaccctgcc
    26701 gacatcgagc gactgaagcg cggcgagttg cgcatggacg aactgttccg tgaagcaagc
    26761 tgggacgagg cgcttgatct ggcggccagc ggattgaaga aggttcgcga ttcggcgggc
    26821 cacggcagcg gctccgcgct ggccggtttc ggctcggcca agggcagcaa cgaagaggct
    26881 tacctgttcc agaaactggt gcgccaggcg atgggcagca gccatgtcga ccactgcacg
    26941 cggttgtgcc atgcaagttc tgtcactgcg ctgctcgagg gcatcggctc cggggcggtg
    27001 agcaacccgg tggccgacgt ggccgaggcc gaactgatct tcctgatcgg cgccaacccg
    27061 acggtcaacc acccggtggc ggccagtttc atcaagaacg cgatcgatcg cggtgccagg
    27121 ctcgtgatct gcgacccgcg ccacacgcag ctgacgcgac gcgccacctg gcatctgcag
    27181 ttccgtcccg acaccgacgt cgcgctgctc aatggcatgc tgcacacgat catcaacgaa
    27241 ggcctggtcg acgagacctt cattgccgcg cgggtcaacg gctatgacgc gttgaaggca
    27301 tccgttgccg aggcgacccc cgagcggatg tccgagatct gcgggatccc agcgtcgacc
    27361 atccgcgagg tggcgcgtgc ttatgcgacc gcacgcagct cgatgatcct gtggggcatg
    27421 ggcgtcagcc agcacgtgca tggcaccgac aactcgcgcg gcctgatcgc gctggcactg
    27481 atcaccggcc agatcggccg acctggtacc ggcctgcatc cgctgcgagg ccagaacaac
    27541 gtgcagggcg ccagcgacgc cgggctgatc ccgatgatgt tgcccaatta ccagcgcgtg
    27601 tcgaatgccg cggtgcgtgc gcatttcgag acgatctgga gcacgccgat cgacgcgacc
    27661 tcggggctca ccgtcgtcga gatcatgcac gcggtcaccg acggcaagat cagaggcatc
    27721 ttcgtcgagg gcgaaaaccc ggcgatgagc gatcccgatc tcgaccacgc ccgtgcagca
    27781 ctggccaagc tggagatcct ggtcgtgcag gacatcttcg cgaccgagac cgcgatgctg
    27841 gccgacgtga tcctgccggc ctcggctcag ccagagaagt ggggcaccta caccaacacc
    27901 gatcgcctga tccagatcgg ccgccccgcg ctcgcgccgc ccgggctggc ccggcaggac
    27961 ctgtgggtca tcgagcagct ggcgcgtcgg ctcggcgtgg cctggaacta ctggcaggcc
    28021 gccgacggcg agggacacgt cgcggtcgag gcgcctgtcg cacgcgtcta cgaagagatg
    28081 cgctcggtga tggacccgct ggccggtgtc ccatggacac ggctgatccg cgagggtgcg
    28141 gtggttaccc cagcgcaccg tgaggacgaa cccggcgaaa gcgtggtctt catcgatcat
    28201 ttcccgaccg cggacggtcg tgcgaccgtg gtgccgacca cttaccagcc cggagtcgag
    28261 ccggtcgatg cggcttaccc gttcgtgctg accaccggac gtgtgctcga gcactggcac
    28321 accggcgcga tgacgcggca cgccagcatg ctcgatgcca ttgcccccga agcgctcgcc
    28381 gtgatccacc cgatcgccgc ggcgcgtctg ggcatctccg atggcgaggt ggtcaacctg
    28441 acgacccacc acggcagcgt tcgcacccgt gccagcctga gtggcgatgt gcagatgggg
    28501 caggtcttcc tgccattcgc attctgggag gccgcagcca acaagctgac tggtgatgcg
    28561 ctcgacgcgg ccgccaagat tcccggcttc aaggtgaccg cagttcgaat cgacaggatt
    28621 tgaagccgac ctgcggtgct gcgccagaat gccggcgtct ctcgacatga actctcccgg
    28681 actcgccact cgacctgaac gcgccgcaga ccggcgcgtc gtgtcggtgc gtgcgccggg
    28741 gcgtctgcat ctcggcttcc tcgacccagg tggctcgctg ggccggcgct acggcagcct
    28801 cgggctggtc gtcgatggat tcgagacgca ggtcgacctg tgcggagcca ctgcacggcg
    28861 cgtcaccggg gcagtgcctt cggcgcaggc cgagatcgac cgcgcggagt cgcatctcca
    28921 tgtgctgcga cggcagaccg gattgacgca gccgctgcac ctgcatctcg agaaggtgct
    28981 cccggcgcat gccggtcttg gctcgggcac gcagttggcg ctggcgatcg gtcgggcgtt
    29041 cgcgctctgg cacgggctcg atgtgccctc ggccacgctg gcccgatggc tgggtcgcgg
    29101 tctgcgctcg ggcgttggca tcggcggatt cgagcagggc ggcctgctgg tcgacggcgg
    29161 gccaggcccc gacggccagc ccgcgccggt gctgtcgcgg gtggccttgc ccgacgcctg
    29221 gcgcgtggtg gtcgtgctcg actcgcaaca ccgaggcctg tcgggtcacg aggagaagca
    29281 ggcgatcgcc gccttgcccg ccatgccgag ggccgttgcg gccgacatct gccaccaggt
    29341 gctgatgcgt gtattgcccg gtgcagcgtg cgccgagttc gcgtcgttcg ctgcgggtat
    29401 cacacgggtg cagcagcagc tcggcgcaca ctttgcgccg gcgcagggtg gcgaggccta
    29461 caccagcacg agcgtcggcc gcctggttcg ttggatcggc gaagcggccg ggggtgaatc
    29521 gagtgcggcg atcggacaaa gctcgtgggg tccgaccggc ttcgcgatcc tgcccagcca
    29581 gcagcaggcc gatgccctcg tcgaagcggc ccgcgccaca ccgctgatgg ctcctcacct
    29641 gacgctgcag atcgtgggtg gccgcaaccg tggcgcgacg ctgaacgacc cgcgtgctgc
    29701 cacggcgtga cacctttcaa gaccgaagaa gagacagcca gagagaggtc cgacatggaa
    29761 cgcccctaca tcctccacat gttcactccg ggccgacaga tgagcccgtt cgacatcaac
    29821 atgtccgccg acgcgggtta ccagatcgtg gttccgtaca gcggtgtcga catcggggcc
    29881 atctccgcac tgacgcagga cgtgatcttc tcgcgcggtc cgaccggtgt ctctcgcacc
    29941 ggcatcttca tcggcgggcg tgacgccgtt ctggcggccg acatgctcga caagtcggtc
    30001 aaggcgatgg tcaagccctt cgtcgtgtcg gtgatggccg acccgagcgg ggcctacacg
    30061 accgcggccg cgatgatcgc ctgcgtcgag gctgcgctgc agcgcagcca tggccacggc
    30121 ctcgagggcc agaaggtcgt tgtcatcggc ggtgccgggc cggtcggccg catcgcctgc
    30181 gtgattgccg cgcaggccgg cgcgcgcgtc tgcctctcga accgcgacgg gctggtcgag
    30241 gccgagaccg ccgctggtgt cacgggagtg cgtttcggcg tgaccctgca gggcgtgtcg
    30301 ggcgagagcc acgacacggt gcacgcgtcg ctggccgacg ccgacgtgat cctcgcctgc
    30361 gcggccgctg gtgtgcaggt gctgtcggct gcggatctgt cgcatgccag gcggctgaag
    30421 gtcgccgcgg acgtgaacgc ggtgcctccg agcgggatcg ccggtatcgg cgtcatggac
    30481 aacggtgtgc ctctggcaga tacgcaggcg gtcggcatcg gcgcgctggc catcggcaac
    30541 gtcaagtacc agacgcagca ccgtctgctg gtccggatgc acgattcgca cacgccgctg
    30601 gttctgagct tcccggaagc gttcgccacc gcgcggcagt ttctgcagga acaggcctgc
    30661 ggtcgagcct gagctgctcg ccgctcccat gacgatgctg gcggtggccg ccatctccgc
    30721 gcgcgcgata accgaagccg ccgcgcgcga tggctatgag gtcgtcgcgc tcgatctgtt
    30781 cggagacgtt gacacccgcg cggcgagcgt gacgtggtcg gcgctcggtg agcctggcgc
    30841 cctgcacatc gacgccgagc gcctgcgttc ggcgctggcg ggtctggcgc gcaatgccga
    30901 cgtgctcggc tggtgcccgg gcgccgggtt cgaaggcgct gccgatctgc tcgagcgggg
    30961 cgcggccgtc ctgccgctga tcggcaacga cgccgccacg atgcggagcg tgcgcgaccc
    31021 gcggagcttc ttcgaggccc tggatcgcct gggcatcgcg catcccgagg tgcgttttga
    31081 gccgccggaa gatggcgagg cgctgcactg gctggtcaag gatttccagg gcaccggtgg
    31141 ctggcacatc cgtcgctggg ccgccggcag tgcctcgcga ctcggtggtt cgccggcgtt
    31201 gtatttccag cgcgaagcgt tgggccggtc gatgtcgctg accttcatcg ccaacggcag
    31261 cgatgtctgc gaactgggct gcaacgagct gacgatccgg cgcttcggcg cgaagccgca
    31321 tgtctattgc ggtgcggtcg gaccagtgat gctgccgatc cccgtggctc ggcaggtcgg
    31381 cctcatcctg cgggccgtcg tggccgagtg gggactgcgc ggactcggca gcctcgactt
    31441 ccttctggag ggcgacacgg tctcggtgct cgaggtcaat ccgcggccgc cggccagcat
    31501 ggcgctttac gagctgcgcc gcttcgaatg cgccacctct ggaacgctgc gcacgcacgg
    31561 cttgatggat gcgcacattc gcgcctgcct gaacggcgat ctgcccgcga tctgccctga
    31621 ggacgccgac gcaccggccg tgcgtggcat cgagatcgtc tatgcgctgc ggcctgtgga
    31681 ggtcaacgcg gccgtgacgg cacagttgtc gcgctggccc ggtgcgcacg acctgcctgc
    31741 cccgggcatc cgctgcacgg ccggcgatcc actgtgcagc gtcgcggccg ttggtgccag
    31801 cgccgacgca gtccgaaccc gattgaacga cagccgcaag atgctgttgc agaccctgga
    31861 gacatcgaca tgaccgacac cctgccgccg ctggcggaca cgcacctgag cgtcaatgcc
    31921 ctcacgcaat cgcgtgtgca gcgcctgttg aacgaagccg aatcgctcaa cgtcaaggcg
    31981 atcgaggatc cgaccggcgt gtgcatcgtc gatgccggca tcgaggcccg cagcagcatc
    32041 gccgccggcc tgctgatcgg cgagatctgc atgggcgggc tcggtgacgt caccttgcgc
    32101 accggtgtgg acgacggctg gccgacctgg gtccaggtgc gcagcgcgca gccggtgctg
    32161 tcgtgcctgg gcagccagta cgcgggctgg agcctggctg ccacgaagga agagaccggt
    32221 ggcaggaagt tcttctcgct gggatcaggc ccggctcgag cgctggcggg caaggaggcg
    32281 ctgttcgccg agctggggta ccgcgaccgg gccgactctg gcgtgctggt actcgaagtc
    32341 gaccgcttgc cgccgaagat cgtcatcgac aaggtgctga acgactgcgg gctgaagccg
    32401 gccggcctga cgctgatcct tactccgacc accagctggg ctggcggtac ccaggtggtg
    32461 gcgagggtgc tcgaggtggc gctgcacaag gcgcacgaac tcggctttgc gctcggcaac
    32521 gtcgtcgatg gcctggcgtc cgcgccgctg ccctcgccga gccccgacgg ggtggtggcg
    32581 atgggtcgca ccaacgacgc gatcctctat ggtggtcggg tgcacctgag cgtgcgcggt
    32641 gacgatgcgg ctgcgcgcga cctggcgctg cgcctgccgt cgtcgaactc gcgtgactac
    32701 gggcgtggct ttgccgacat cttcaaggaa gcgggctacg acttctacaa gatcgacggc
    32761 gcgctgttcg caccggccga ggtctgggtc agcaacgtgg acagcggcaa cacctggcat
    32821 gccggcgcgc tgaacatgcc gttgctccag aagctctggc ttcaacaggc ctgagccatg
    32881 cgcgttgcca tcctgaccga cgagaccggc tggcacacgc ggcagcttca gaccgccctg
    32941 cggtctcgcg gtgcggtcgg gcggtgtgtg gatctggccg attgccgcat cgacaccact
    33001 gccgcatggc acgggctggc gatccccggc tatggccgcg agctgcccga cgcggccctc
    33061 gtgcgaggca ttgccggagg cactttcgag caagtgacga agcggctggg tgtgctgcac
    33121 gcgttgcgcg aactcggggt gccggtctac aacgacgccc gcgccatcga gcgcagcgtc
    33181 gacaaggcca tgaccagtct tctgctgcat gctgccggca tcccgactcc gccgacctgg
    33241 gccaccgagt cggctgcgca ggcacagcgc atcgcgattc gcgaaggcgc ggccggccac
    33301 gccctcgtcc tcaagccgct gttcggctcg cagggcaagg gcctccaact ggtgggcgag
    33361 gtcgacggga tacaccaccc gctgccgccg ctcgacaagt cctatggctc gatggcttac
    33421 ctgcagcgct tcgtgccacc ggtctgcgaa cccggcttcg attggcgcgt gctggtcgtc
    33481 ggcggtcgcg cgatcaccgc catgcgccgt gtcagccggc actggatcca caacgtcgca
    33541 cagggcgcgc gctgcgagcc gcaggccatg acccggccgc tggctgcgct ggcagagcgc
    33601 gcagcggccg cgctcgctct ggattacgcc ggcatcgata tcgtgccggt gcgctgcgat
    33661 gacaacgacc ccgacgagtc cgacatgcag gtgatcgagg tcaacggcgt cgctgcctgg
    33721 cacggcctgc agcgagtcac ggatttcaac attgccgctg ccctgatcga cgacctgctg
    33781 gatcgcaagt gcgccggtgc ggcggctcgc tccggcgtgg gtccatgcgt cgcgccggaa
    33841 gttcgccgcg cgtgagttcc gacaccgggg gcgcgatcga ccgcgcccgg gccgcctacc
    33901 tgcgagcgtg cgaactcgat atcgcggttc gcaagcccgg aaacgtgagc atcgactcgc
    33961 ccggtcacgg catgcaggcg cggcagttca tcgacagcgg tcgtgcttcg gctgcagcgc
    34021 tgttcgcgcg tggtgcccgc gtcggcgagc gcatcgaagc ggcgattgca gcgacctggg
    34081 cggtggctgg ttgcaacacc aatcttggca tcgtgctgct ctgtgcccct gtggcggcag
    34141 cggtggaacg gtttccgcgc gccgcctccg agccggaaat gaggttggcc atcgaagcgg
    34201 tgctggccga tctcgacgag accgacgcgc gtgctgcata ccgtgccatt gcgcgggcca
    34261 accccggtgg tcttggcagc gccccacgcg aagacgtacg cgcggaaccg acggtcacgc
    34321 tgcgtgcggc gatggcgctg gccgcccatc gggaccggat cgggctgctg tatcgggacg
    34381 gatatggcgc gctgttcgac atcggggttg ccgccctggg cgatgacttt cgcctgaccg
    34441 aagcgcaagc cgcgcatcgg tcagccgttg ttgacgtggc gctgccggtg cccccgacgc
    34501 gtcgagcggt cctgcgctgc tatctcgcgt tgctgagcca cgcgccagat tcacacattg
    34561 ttcgaaaacg cggggcagga ccggcacaga ctgtcatggc gactgcggcg cgctgggccg
    34621 tcgacccccg actgaccaca gccgacccgg ggctcgagct ggatccggta ttcgtggaat
    34681 gggatgaatc cctgaagtcc gaaggcctca acccggggac cacggccgat atgacggtcg
    34741 cttcgatgct tctggcgttg atcacgcagc cgcgatcgcg cgcgtaaatc ggcaccgccg
    34801 tgcaggttcg ctcccgggtt ctggtgggtt tgccagtggc acggatcgtg atactgtcat
    34861 tgaccggctg gagcaccagt ccgtgagaca agacctcttc atcccagaca ggagctagaa
    34921 aatcatggca aaaatcgacc gcttgatggt tggcgaatcg ctcgtcggcg acggcaacga
    34981 agtggcacac atcgacctga tcatcggccc acgcggcagc gccgctgaaa ccgcgttcgc
    35041 gaacgctttg gtcaacaaca aggacggatt cacgtcgttg ctggctgtcg tcgcgccgaa
    35101 cctgctgacc aagcctgcca cggtgatgtt caacaaggtc accatcaagg gtgccaagca
    35161 agccgtccag atgttcggac cagcccagcg cggcgtcgcg atggcggtgg cggactcggt
    35221 cgaagatggc acgattccac ttgacgaagc cgacaacctg ttcatcagcg tcggtgtctt
    35281 cattcactgg ctggccgaag acgacaagaa gatccaggaa tacaactacc aggctgtccg
    35341 cgagtcgatc aagcgcgctg tcaacgggtt gcctaccgct gccgaagtcg ttgccaagaa
    35401 gggttcctcg ccgcacccat tcgctgcaaa ctgagttcgc tttccgcggc ccacaaggcc
    35461 gcagaaatcg atcagaaaag ccgatcggaa catcgatcgg cttttttgtt ggcgggacgc
    35521 aagccggtcc ctcccgcggt caattgcccc caggcaccat tcctgcgccg atcacgcgca
    35581 agacacccgc agcacggggt gtctcgggct gcgccgccgc cgagcgctcg atcaccacac
    35641 cgacggcggc cacatcgtcg aatttcctgg gcttgaccac cttgacccgc acccaagccg
    35701 cgccgaaatc gatgatcagc atgtccgcga tggtttcggc gaatgattcg agcagggtca
    35761 gcttgtgctc gcgataaagc acctgcagcc gttcgcggac caccccgtag tcgatggtgt
    35821 cggcgatctg gtcggtgtcg caggccttgg atcgaggcaa cccggcgtgc aagtcgacga
    35881 cgatggtttg tggtcggtgc agttcggaat catggatacc gatgacggtc tggcccgtga
    35941 acccttcgat gaagatcacg tccatggtct gcgttgcatc ggcggccgcc acgcgacggg
    36001 cacctggcac cgctgcggca gccagtgcag atggaaagcc ggggtcgggt cgggtggtca
    36061 agaggttctc cagggcatgt actttgtctg tatcgaaact gccgcgacaa tgcgatattg
    36121 acacacgcgc tgcaagcatc gagccacggg cagtcgcacg cggcccgatg cgccggaaga
    36181 cagccagcca cgattcgaca agcacaagca ggcgccagag gctcccatcg acgtggggcc
    36241 ccgcgtgttg cgaagaagag cccaggagcc gttcatgcca ttgagtctgc agaatgcaga
    36301 gcatcacgcc gagcgcatcc tcgacgcgtt gcgtcgcggc gagcgaagcg tggccaccaa
    36361 cgatatcgtc acccgctcat ggggacgctg cgtccacgac taccgcctgc atcccgacca
    36421 gccgcacgag ccgacggtga tagcccgcag tgcgctgcag gagcggcgcg aacgcatcgg
    36481 tgacgtgatc gaatgcgccc gccacgagat gtcgaccttg ttcgaacaac tcgcagattc
    36541 cgaggccgct gtcgtgttga ccgacaccga tggcgtcatc gtgcacatgg tcgcggctcc
    36601 agccttcgcc gccgatgtgg ccccgttggg tctgcgcccg ggtgcggtct ggagcgaaac
    36661 tgcagccggc accaacggaa tgggcacctg tctggcctcg gcgagtcccg tggccgtgca
    36721 gcgcgaggat catttctaca cgcagttcac ccagctcacc tgctcggcgg ttcccgtgtt
    36781 cgacccttcg ggcgagctcg cagccgtgct cgatgtgacc agccgttcga cgctgatgca
    36841 gcagcatctg ctggtgctgc tgggcatgac ggcgcggatg atcgagaacc gtctgatcga
    36901 ccagcgtttc ccgcttgcgc acccgatgca cttccacagt cgaccggagt tcgtgtacac
    36961 gctgcatgaa ggcaagcttg cggtggccgc ggacggccgc atcgttgccg ccaaccgcag
    37021 cgccctgttt cagctgggcg tgcagacggt cgacgacatc cgttcgcggc gcatcgacga
    37081 cgttttccag acctcgctcg gcgaactcct gcagcgcagc gccacgtcat ccttccatcc
    37141 ggtggtgacc tttcgcgcca atgcgtcgaa ccggttcttt gccgtcgcgc ggcgcccggc
    37201 tggcggcgcg atggccgccc cgttcccgct gccgatgggc gagatgcccg gcgcagcggt
    37261 ctcgacgatc acgaccgggc ggccggcgat gcggccagcc gcgtcgcgcg cggccagtcc
    37321 ttcggtcgat gccaccttca aggatgatcg tctggtggct cacctgaaga ccgctcgccg
    37381 ggtcatcgcg cgcggcacgc cggttctgct gtgcggcgag acgggatcgg gcaaggaagt
    37441 ctttgcgcgg gccatccacg acgggagccc gcacgcgacg ggcgctttcg ttgcgatcaa
    37501 ttgcgccagc ctccctgaga gcctgatcga ggccgaattg ttcggctacc gcgccggggc
    37561 cttcaccggt gcgcagcgga ccgggcggcg cggcaagatc ctgcaggccg atggaggcac
    37621 gttgttcctc gacgagatcg gtgacatgcc gctcgaactg caggcgcggc tgctgcgtgt
    37681 gctcgatgag cgccaggtca ccccgctggg tggcgaggag atgcacgacg tcgacttcca
    37741 gttgctcagc gccagtcacc gacacctgcc gacgctggtg cgagaagggc gcttccgcga
    37801 agacctctat taccgcctgg ccggcatcga gctggagctt ccggcgatgc gcgatcgcag
    37861 cgacaagcgg gagctgatcc gcagtgtgct cgcctcggaa gcggggtctc atgcgtcgat
    37921 cagtcgggaa gccgagcaac tgctgatggc ccatccctgg cccggcaatg tgcggcagtt
    37981 gcgccacgtg ctgcgcagcg ccgcagcgct tgccgacggt gcggcgatcg ggccggagca
    38041 cctgccgtcg ctggtggcct caccatcggt gtcgggcgct cgtgcgtcgt cggcccaagt
    38101 cgatggcgaa acccgtcctg cagcggcggc atcctcggcg gcgccggtgg agccgaagct
    38161 caatgccatc caggccagcg aaagaatgat gctgatgcag ttgctggagc agcaccgctg
    38221 gaatctcagc aacgtggcca aggcactcga catcagccgc aacacgctct atcgcaagct
    38281 gcgcaagctg cacatcgaca tttcgcatcc ctgaccgcct cagcctcgaa ccgcatccgt
    38341 gacgctcgcc catgaccgcc ctccggtaac cccgctgtcc gacggcgaca ccgtacccgt
    38401 cgatccgacc ccacgttcgc gcttcgcctg gtgccttacc ggctcgggac attttctcga
    38461 ggagtcgatg gcgatcgctg cgcgcctgcc gcaggtcgat ctgttcctgt cggcggcggc
    38521 cgaagaggtg ctgccgatct accagattcg catcgaggat ctgcggccga cgttccgcgt
    38581 gttccgtgac aagacggcca gctcggtgcc ggtcggcatg ctctacgacg acgtctatca
    38641 caccgtgatc gtcgcgcccg caaccagcaa cacggtggcg aaatgcgcct tcggtatcag
    38701 cgattcgctg ccgaccaaca tgttcgctca agccggcaag ctcggcattc cgggcgtcgt
    38761 cttcgcctgc gacaccgagc cggtggtcgt gaccaagtcg cctcacgact gggtcacgct
    38821 gcgcccgcgg cgcatcgagc tcgacaacgt cgagcgcctg cgcggcatcg accactgcca
    38881 tgtcgtgacc accccggccg aactcgaagc ctggctcgat gcgcggttgg cccaactttc
    38941 gctggcatgg aacacgtcgt cttcctgacc gggcggcttg cgcagccgag cctcgaacgc
    39001 ctgttgtccg gcatcgaggc cctgccgttc acctgggagg tgcgcgagat cgggctgcag
    39061 gttgcggcgc tgatgaccgc cgatctcatc agccgccgtg tgccggcgcc ggtcgtgacg
    39121 tcgtgcgagg acggatgtca gcgccgcgcc gacaggctca tcgtgcccgg tcgctgtcgc
    39181 ggtgacctcg aggcgctgac gcagcattac ggagtgcctg tgctgcgcgg gcccgaagag
    39241 ctgaaggacc tgccgcgcta cttcaaccgt gctgcagtcc cgatcgacct gaccgagtac
    39301 gaggtcgcga tctttgccga gatcgtcgac gctcaccgca tcggtgtcga tgagatcgtc
    39361 gggcgtgccc gcgcgctggc cgccgatggt gccaacgtca tagacctggg ttccctgcca
    39421 gacacgccgt tcgcccacct cgaggacagt gtgcgtgccc tgaaggccga agggtttgcc
    39481 gtcagtgtcg attcgctgaa ccctgacgag ttgctgcgtg gcggtcgtgc cggggcggac
    39541 tacctgttga gcctgacact cgacacgttg tgggtggccg acgaggtgcc gtcgacgccg
    39601 atcctgatca gccgcacgcc gaccgatgag gattcgctgt accaggcgat cgagttgatg
    39661 caggacaagg gcagaccgtt tctggctgac acgatcctcg atccgatccc gttcggcatg
    39721 accgcagcca tcgtgcgcta ccaccggctg cgcgagcgct acccggacgt cgcgatcatg
    39781 atgggcgtgg gcaacgtgac cgagctgacc gaggccgaca ccagcggcat caacgcggtg
    39841 ctgttcggca tctgcgccga gctcaacgtg gccgcggtgc tgaccacaca ggtcagtcag
    39901 catgcgcggc gtgcggtgcg cgaggccgac tgggctcgcc gcatcatgca tgcggctgcg
    39961 cgccaccaga cgctgcccaa gggcatgaac gacgcgctga tgacggtgca cgcaaagcac
    40021 ccgtttcccg acacgcccga ggagatcgca gcgacggccg ctctggtgcg ggacccgaat
    40081 ttccgcatcc aggtttcgcc ccagggactg catgtttaca accgcgacgg aatgcgcctg
    40141 gggcagggcg ctttcgagct gtggccgcag ctcaagctgg aagacgacgc agcgcacgcc
    40201 ttctacatgg gtgtcgagct cgcgcatgcc gagactgcgc tgcaactcgg caagcgctac
    40261 gtgcaggacc agcccctgga ctggggttgc gcggtcgagc gtgcccccgt cgatctggcc
    40321 ggctggtgtg cgcccggtac gacgatgcag accgggaaga tgcgtggcaa gctgcccgcc
    40381 gacccagccg acgaaaccag cgagcggacc tgacgatgaa tgacgtgatt tacgagaccg
    40441 tggtgaccac gttgtcggcc gatggcgttt cgcatgtggc gccgatgggc atccgctatc
    40501 ggggcgacga ggtggtgctg atgccttacc gtccttcgac gacgctggac aacatcgtgg
    40561 caaccggctg tgccgtcctg aacatcgttg tcgatacgcg ggtgttcgcg ggcagcgtga
    40621 ccggacgcaa ggtctgggac acgttgccag ccgagcggat ccctggcgtg cgactggcct
    40681 gtgcgctgag tcacctcgag ttgcggctgg tgtcacgaca ggacgaggag cagcgaaccg
    40741 tgctgcacct ggcgcgcgag cacgcgtgcg tgcatgcgcc gtacatcggg atcaaccgtg
    40801 cgcaggcggc ggtggtcgaa ggcgccgttc tggtcagccg cctgagcatg ctcccggcgg
    40861 gtaaggtcga cagcgagatg gcctacctgc agattgccat cgacaagact gccgggcccg
    40921 aggagcgcga ggcctggggc tggctgagag aagcagtcgc gaagcaccgc cgacacgcgc
    40981 agcagcagca atgacgaccg ccacgatgcg catgctggtc agcgtgcgcg atgtccagga
    41041 ggcgctggag gcggccgagg gcggcgccga cttcatcgac ctgaaggagc cgcgagaggg
    41101 tgcgctgggc ggacttccct tgaacacgat ccgcgaggtc gtctgcgcgc tgcgggcgca
    41161 ccacctgcgc ctcccgatca gcgccaccat aggagacctt ccgatggaag acgtggcgca
    41221 gatcctgacc cgggtcgccg acgtgggctc ctgtggcgtc gactatgtca aggtgggcat
    41281 agagcgcgaa actggtgcgg cggctgtcct cgacgccctt gccagctgcg gttggcccgt
    41341 ggtgccggtg ttcatcgccg accgcgggct cgacaacgag ctcgtcacgc gggcgtgcaa
    41401 cattgcaggt ggggcaggcg ctgcatttcc ggccatcatg gtcgacaccg ccgagaagca
    41461 ggcgggcagc ctgttcgaat tgctgagtcc ggaggccttg cagcgtttca tcgacgcggt
    41521 gcgtggagcg catgcgatgg tcgggctggc cggcgccttg cgcctggcgc acgtcgagcg
    41581 actggcggca ctgcagcccg acttcgtggg atttcgcagc gcggtgtgcg tgggcgatcg
    41641 ggcatcgcaa ctcgatgcgg gccgggtgcg cgatctgtcg gcgcggctgc gcgctgcagt
    41701 gcaccacgag ctgcacgcct gagtcgacgc ccgggtcagc cgcccttgtc gtggcggacc
    41761 ttcagctcgc ccagcatcag cgagcaggta cgctggccga ccacgtcgat gcgcagggac
    41821 tggccttccg ccagcatcgc gaaccggcga tcgacggcgc ccctcgcact cagctgatcc
    41881 agcgcggccg atggatcgat ctcgcatgac ttgaccacga tcagccgtgc tgcgcccagc
    41941 cgcagggcga gtccgagcgc gatgctgtcg gatgtgacgt cccagttcgt gtcggcatcc
    42001 gcgcggtcac gctgcagttc gagcggcgac cagaccgcga ccgcgccctg ccgaagcgcc
    42061 tggtgcagcg ccgcctcgcg atcgcacagg cgcagcgccg gattcaggct gcgcagcagg
    42121 tgcgcggtct gggacatcgc gagcaaggcc atgttgtggg ccgtcaggtc gtcgaagcgc
    42181 cactgcgcct gcgccccgcg caccgcatcg gcgaaccttc cgcctcccgg gacgatcacc
    42241 acgcggcccg caccctcgcg aaccagcagg tccagccagt cgcgcagggg cgatgtttcg
    42301 ggtgcctgct cgcacaggct gccaccgagc ttgaccaccc acatcagcat ggctcccgcg
    42361 cgtacaggct ggcgaccgca acgctggggg cgcagacctg agcccagtcg gctttgctgg
    42421 cgtccgcggc tccatcgtcg gtggctgcga tgcgcgcgac atcgcagccg taggaccgaa
    42481 ggggccaacc cgtcagcgcc ggcacgcgcg ctgccaggtc ggggaccaga aaggcgccgc
    42541 aacccgcggt gacgatcgtg ccggccgatg cggtcccgca ccgcgtggcc atcatgcgag
    42601 tcagcgaggc tgcgatctct tcgatctggg cctgccgcca tgccttcgcc agcgcgagcc
    42661 actcgtcgtc ctcggcatct cgcgcgtcga gtccgatcat ccgtgcaagt cgctgccggg
    42721 ttgccgtgcg gtccttcgac cgtgcgtcag cactcggctg ctgatcgtgt gccgggtcga
    42781 gctcgccggt catccggtag acgtccgccg tggtcgcgaa ccattcgttc atcacgttga
    42841 ccggggggcc gcgaaaggca acgtgcctcg ccagtgcgca cagtggcgtg cgcacgacgc
    42901 cgtgatagac cagctcgccg cttgccagcc gctcgtggtc gctgcggctc gcggtttgca
    42961 cgcgcccatt cgccagcacg atcaggtcgg tggtggtgct gccgatgtcg accagcaagc
    43021 cgtcgcgaaa gaccgtcgcc gcatgatgcg cggtggcgag ccaattggcc gatgcgatct
    43081 gcgtccagca ccgcgctgcg tcggctggcg caatccagcc caggtcgccg ccatagagtc
    43141 gcacctcccc gcgcaacacc tgcgccagcc gatccgcgat gcgctgcacg ccctcttcgc
    43201 gatgctcgaa tgcatcgacc atttcgccgc tcatcgtgat cgcgtgtctg gtatcgctgc
    43261 ccagcgcggg ccagcgtgcc tgggcttgct gcagcgcgat gtcgatctga tccagcccct
    43321 gccacagcgg gcaggcccat tgcgccacat cgatgacctg accatcgcgc gtgaggcagg
    43381 ccttcaggtg cgcgccgccg acgtcccagc cgacgatgaa cgtgtcagag cgctgcatcg
    43441 gtgatttcgg tctggagatg cgctgcttcg tgtgccgaca ggatctctgc cgccaggttg
    43501 cggcccagcg cggccgacat cccgacgtac gcgctggtga cgcgcgggtt cacctcgacg
    43561 accaccggtc cgcgccgggc atgccacacc agatcgatgc cgacccagcc ggccagaccc
    43621 ggtatggcgg cgcagacgtt gcgggccgtc tgctcgaacg cgtgccgctg cgcggcatcg
    43681 aagtcgccgg cgccgatgtc gacttcgatc agagacaccg cgccttcccc atcgatcgcg
    43741 agccgctgcc gattgaccgc gagcagctct gcgcagtcgt tgtgaacctg cagcgacagg
    43801 ctcagcgcgt ctccttcgac ccagggctcc agcgtcgcgg tctcgccgcg ctgtccgcgc
    43861 cgctgccaat ccatcagtgc ggccgcgtga tcgccatgcc gacgtgtgtc caggctgcct
    43921 gcgccgtcgt cgggcttgac gacccaatgt cgcacggcgg catccgtcat gaaatccagt
    43981 ggagtcgcga tgctgtgctg ttgcagtcgg gccagggtgg cccgcttgct cgatgcgagc
    44041 cggatcgc
//
LOCUS       DAOORV010000013        40850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1094_length_40850_cov_7.348915, whole genome shotgun sequence.
ACCESSION   DAOORV010000013 DAOORV010000000
VERSION     DAOORV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 40850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1094_length_40850_cov_7.348915"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /gene="gspD"
                     /locus_tag="PKV56_03375"
     CDS             <1..1949
                     /gene="gspD"
                     /locus_tag="PKV56_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOX66882.1"
                     /translation="PREAYLNYLAALRGLGFTVVEVGGLFKVVPEADAKLQSGTVSVG
                     EVSRRGDQVITQIYRLTHENANNLVAVLRPLISPNNTINANPGNNTLVVTDYADNLQR
                     IGKIIAAMDTPAAGDVEVITLQHAVASDIAALVQRLTDTATGAVQGATVASSASVLVD
                     ARSNSLLVRAPNPARLAAIRALVDKLDQPIKGGNAAGNIWVVYLKNADAVKLAAVLRA
                     AYAGGSGTSSSGGSSSTPAAPQSSPNTLGTSTSTSSGSTSSASAQATAPVTQSAGPST
                     GGFVQADPATNSLIITAPEPMYRQLRSMIDQLDSRRAQVYIESMIVEVSGDDSADFGF
                     QWQSIGNSNDKYIGGIGTNFGTGGTNILTNQGSTTAVGALKGLGQGFNVGVVRNFGGT
                     FGLSAVARALQSRADTNIVSTPNLITLDNEEAKIVVGSNVPFITGQYTNTGGNSNNSP
                     FQTIERKDVGITLRIRPQIGENGTVRMTLFQESSSLAAGTAPGTSSAGPTTNKKSIES
                     TVVVDDGQIIVLGGLIEDSIVVDKSKVPILGDIPYFGALFRSETRTKKRTNQMVFLRP
                     VVMRDASSSNKLSVDRYDLIRGEQKDVQLPTSPMMPIGGVPVIPPLKPTEDPSQPLTP
                     PANRPPPSGLVPPTATPDGAATAN"
     gene            1965..3386
                     /gene="gspE"
                     /locus_tag="PKV56_03380"
     CDS             1965..3386
                     /gene="gspE"
                     /locus_tag="PKV56_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOX66883.1"
                     /translation="MGTRHPLPYAYARANTVLLEDDGDRLVLWAPENVSLTALGEVLR
                     RFDVDALERESSSTLANRIAVAYAGSESSAATVIGEVESAVDLSRMMQELPAIEDLLE
                     ASNDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAA
                     LISRLKIMAELDISEKRLPQDGRISLRIGGRAVDVRVSTLPSSHGERAVLRLLDKGET
                     RFTLESLGMSGDVLTSFDRLVQQPHGIVLVTGPTGSGKTTTLYASLGRIDTSTTNVLT
                     VEDPVEYELGGIGQTQVNPKIDLTFAKALRAILRQDPDVIMIGEIRDYETAQIAIQAS
                     LTGHLVLATLHTNDAPSAVTRLTDMGVEPFLLSSSLLGVLAQRLVRKLCVSCRKVDAQ
                     GRYHPVGCADCGQTGYKGRTGVYEMMVADDAVRSLIHSRAAESQLLVAAEHAGLRSMR
                     EDGERLVIEGVTSLEEVLRVTRE"
     gene            3388..4602
                     /gene="gspF"
                     /locus_tag="PKV56_03385"
     CDS             3388..4602
                     /gene="gspF"
                     /locus_tag="PKV56_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOX66884.1"
                     /translation="MPAYKFEALTAAGKSTTGLVEADNARAARAQLRAQSLVPLDVTQ
                     VASADAKGQGLRFRRRVFSTTGLAVWTRQLAGLVGSGLPLERALTALSDEAEDAPQRE
                     LVAHLRSEVNAGSPFARALASAEREFDDVYRGVVAAGEQSGALGQVLERLADDLEERQ
                     ALKAKLIGATLYPAIVSVIAVVIVIFLVTYVVPQVASVFTSSKRALPGLTVAMLAVSG
                     FLRSYGWLLLLAIGGGTATLMLMLRNPSFRERFDAAWLNLPLVGRLARGYNAARFAGT
                     LAMLAGAGVPILKALQAAAETLSNQAMRADALDALVRVREGAPLASALAGKKRFPGLL
                     AMFARLGEQTGQLPQMLDRAAKQLATEVQRRAMQMATILEPLLIVAMGGVVMLIVLAV
                     LLPIIQLNTWVK"
     gene            4830..5831
                     /locus_tag="PKV56_03390"
     CDS             4830..5831
                     /locus_tag="PKV56_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX66885.1"
                     /translation="MSASHELVHVSATRGGIPIARMRSLYRVDDVGRKLDKLPHKEHE
                     NLRSTYERMIELGPERFQVKPSGLPVMDHLYDDLPNFCEVLDDVKRQLALCEDSRDAL
                     EITPLLLLGPPGVGKTHFARELSQLLGTGMGFISMSSMTAGWVLSGASSQWKGARPGK
                     VFETLVDGQYANPVMVVDEIDKAGGEHAYDPLGALYSLLEHDTAGSFIDEFAEVPIDA
                     SQVIWVATANDARGIPDPILNRMNVYEVQAPDREAARHIAAKLYSSIRASHDWGSRFD
                     EQPSSDVLDRLCEMAPREMRRAWMTAFGNAKLERRSQVSTADLPDAHQRRAPMGFVQ"
     gene            5890..6813
                     /locus_tag="PKV56_03395"
     CDS             5890..6813
                     /locus_tag="PKV56_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOX66886.1"
                     /translation="MPVSLDGQLVVAISSRALFDFEEENEVFEASDDRAYMRLQLERL
                     DVTAKPGVAFSLVNKLLAFNRAPASEAGEASPTQRVEVVILSRNDPVSGMRVFRSSKQ
                     HGLPIQRGVFTRGHPPWRYLQPLNANLFLSTNEADVRSALDAGTPAARVFPNSARASA
                     AHPNEVRIAFDGDAVLFSDEAEQVFQRAGLNAFQEHERDRAEKPLAAGPFKPLLEALQ
                     RLQRAATPSMSIRTALVTARSAPAHERAIRTLMDWDIEIDEAMFLGGLAKGEFLREFE
                     PDFFFDDQTGHIENAAPHVPAGLVASGVLNR"
     gene            complement(6825..8081)
                     /gene="tyrS"
                     /locus_tag="PKV56_03400"
     CDS             complement(6825..8081)
                     /gene="tyrS"
                     /locus_tag="PKV56_03400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOX66887.1"
                     /translation="MSHTDRLEPSVSAATSYPVTDRVRDAFRATRRGCDELLPEADWL
                     SKLARAEATGVPLRIKLGLDPTAPDIHIGHTVVLNKLRQLQELGHTVIFLIGDFTSMI
                     GDPSGRNATRPPLTREQIEHNAKTYYQQASLVLDPERTEVRYNSEWCDPLGARGLIQL
                     ASRYTVARMMERDDFTKRFKAGTPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNL
                     LVGRALQSEYGQDPQCILTMPLLEGLDGVEKMSKSKGNTIGISEAPNDMFAKLLSISD
                     TLMWRYYTLLSFKSEEEIARLRNAVEGGRNPKDAKVLLAKEITARFHSAAAADAAEND
                     FNLRARGGIPDVIPELAVTGAPLGIGALLRESMLAPSSSEALRLVDGGGVRVDGNVVS
                     DRGLKLSAGTYVLQVGKRKFARVTLS"
     gene            8394..9725
                     /locus_tag="PKV56_03405"
     CDS             8394..9725
                     /locus_tag="PKV56_03405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX66888.1"
                     /translation="MRKHPRQLYSAIVLALAGFGATAFGVAPLSADNSGIAQSWVTES
                     VIPSDLAGQVLSLSQQDLSLVRNDVTRPGDTADSLLRRLNVDDAQALVFLRANRVARE
                     LVSGRGGKMVQVRTSGSGELDELVARYPAASSAQSNTHFTRLRVTREGEQFAARLETV
                     PLASQTRMGSGVVRNSLFNATDEAGIPDSVATQLAEVFATDINFHRDLRRGDTFSVVY
                     EALTADGEPITWNDSAGRVLAAEFVNKGKVNSAIWYESTEAGRRGAYFDLEGRSKQRS
                     FLASPMQFSRITSGFAMRFHPILQNWRQHAGVDYGAPVGTPVRTVSDGVVEFAGWQNG
                     YGNVVVVKHAADRSTLYAHLSRIDVRKGQRVEQGTYLGAVGATGWATGPHLHFEFKVG
                     GAQRDPAAIASLSETVALAPASRAQFATWAQSAKAQLSAAETMAQASISFE"
     gene            9795..10940
                     /locus_tag="PKV56_03410"
     CDS             9795..10940
                     /locus_tag="PKV56_03410"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOX66889.1"
                     /translation="MQRTVADLFIGLMSGTSLDGVDGVLVEFGSNDEILSIAVRAHVH
                     RPFPLEFARELLSLNVTGADELHRAALAGNALAGHYAAVVHDLLKKASIAASEVTAIG
                     AHGQTVRHRPGEFDGIGYTVQLNNPALLAERTGIDVAADFRSRDVAAGGQGAPLVPAF
                     HRAAFGKAHETLTVLNIGGISNLSVLASDGSTLGFDCGPGNALMDHWCALHLGTAYDR
                     DGNWASSGSVNPTLLTAMQGDPYFARPAPKSTGRDQFNADWLDRCLQQNGGSAALAPQ
                     DVQASLAELTAWCCEQSVLLQSPEATRLLVCGGGACNTHLMRRLAARLSWLDVQTTAE
                     RGLPVDQVEAVAFAWLAQACVKRRPGNIPAATGAAGSRVLGAIYPAE"
     gene            complement(11027..11407)
                     /gene="erpA"
                     /locus_tag="PKV56_03415"
     CDS             complement(11027..11407)
                     /gene="erpA"
                     /locus_tag="PKV56_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOX66890.1"
                     /translation="MNAASELTHLAATQDEPPAPLVFTDSAAAKVKELVDEEGNPDLK
                     LRVFVQGGGCSGFQYGFTFDEVLNEDDTRMDKNGVTLLIDAMSLQYLVGAEIDYKDDL
                     QGAQFVIRNPNATTTCGCGSSFSV"
     gene            complement(11513..11905)
                     /gene="rpsI"
                     /locus_tag="PKV56_03420"
     CDS             complement(11513..11905)
                     /gene="rpsI"
                     /locus_tag="PKV56_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOX66891.1"
                     /translation="MIGNWNYGTGRRKSSVARVFMKKGTGQILVNGKTVDEYFGRQTS
                     IMIVKQPLMLTANDAAFDVKVNVHGGGESGQAGAVRHGITRALIDYDAALKPELSRAG
                     FVTRDAREVERKKVGFHGARRRKQFSKR"
     gene            complement(11916..12344)
                     /gene="rplM"
                     /locus_tag="PKV56_03425"
     CDS             complement(11916..12344)
                     /gene="rplM"
                     /locus_tag="PKV56_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOX66892.1"
                     /translation="MKTFSAKPAEVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDFIVIVNADKLRVTGNKANDKVYYRHTGYPGGIIATKFKDMQAKHPGRALE
                     KAVKGMLPKGPLGYAMVKKLKVYAGGTHPHAAQQPKPLEI"
     gene            12752..13468
                     /locus_tag="PKV56_03430"
     CDS             12752..13468
                     /locus_tag="PKV56_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HOX66893.1"
                     /translation="MIAIGAAGAILGAWMVWAWLSPRDRAQPLPNLWAISPRPVFNAN
                     ERRLYRQLREAFPQHVVMPKLPLVRLCQPDVQEQVQYWYGLLGSTHATFTVCSSSGKA
                     LLAIDLESRRSRSKRNLEIKEAVLKACQIKYFSIAANGMPTVAALQLLLPHTGSEAPS
                     AVTRDDVARGPVGESINEPFASTRPSSSQPLKGTGRWQDSSVFMDSFFSPDSRMDPGG
                     DVDLPHGASESTAPSSAAGH"
     gene            13490..14527
                     /locus_tag="PKV56_03435"
     CDS             13490..14527
                     /locus_tag="PKV56_03435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOX66894.1"
                     /translation="MHSSPSPDPALQTPFSGLTPSVVLDALDSVGLAGDGRIFQLNSY
                     ENRVFQVFLSDGRAVVTKFYRPNRWTDAQILEEHSFSAELAAEEIPIAAPWALEADTG
                     SAIQCTLIGAPATLARINTPDGDYRFSVTERKAGRAPELEHADTLEWIGRFVGRMHAV
                     GARKPFAVRRSLTLDEFGWGCRDWLLEHGNIPPEIAPQWKAAVDAALTLASRGFERLP
                     DIQNIRLHGDCHAGNVLWTDAGPHFVDLDDCLTGPAVQDLWMLLSGDPTAARVQMRSL
                     LTGYEQFMDFDDRQLALIEPLRTLRMVHQSAWLAKRWSDPAFPPSFPWFASGAYWQQS
                     AQQLLQQVEQG"
     gene            complement(14524..16407)
                     /gene="htpG"
                     /locus_tag="PKV56_03440"
     CDS             complement(14524..16407)
                     /gene="htpG"
                     /locus_tag="PKV56_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOX66895.1"
                     /translation="MTKQTLSFQAEVKQILHLVTHSLYSNKEIFLRELVSNASDACDK
                     LRFEALNHPELYEDAPNLEVRVSFDAEAKTLTIHDNGIGLSAEEAVANLGTIAKSGTR
                     EFMAKLDGDQKKDAQLIGQFGVGFYSGFIVADRITVESRRAGAQPDEGVRWSSEGTGD
                     FEIESIVRAERGTSVILHLRDGEEEFLSGWKLKSIIGKYSDHVSLPILMQKEEWDAEA
                     AKQVKRDEWAPVNKAAALWTRSKSDITDEQYNEFYKQISYDTEAPLAYTHNRVEGRSD
                     YTQLLYIPAKAPFDLWNRDKRGGVKLYVKRVFIMDDAEALMPVYLRFVKGVIDSADLP
                     LNVSRELLQESRDVRAIREGSTKRVLTMLEALANSEDEAERARYAAFWKDFGTLLKEG
                     VGEDHVNVERLAKLLRFASTQADEGVSFADYVSRMKEGQESIYYITADSLAAAKGSPQ
                     LEIYRKKGIEVLLFTDRVDEWMLSHLHEFDGKSLQSVAKGAVDLGSLQDEQEKKQAEE
                     ASASFKPVLDRLKETLRDRAKDVRVTTRLVESPACLVVEESDMSGHLARLLKQSGQTG
                     VPASKPILEVNAQHALVSRLDKGEHFDDLAHILFDQAVLAEGGQLEDPADYVRRVNSL
                     LLA"
     gene            complement(16507..17667)
                     /locus_tag="PKV56_03445"
     CDS             complement(16507..17667)
                     /locus_tag="PKV56_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX66896.1"
                     /translation="MADPARPASPSTPWSSPLAALRRDRFFWVLLLVLAGLAAAQPQI
                     IPTYPMLVDWATIAALAGLLVLTQAVESSGALAWLGRALVRRMSTERVAALSLVSAAA
                     ALSTVLTNDVALFVVVPLTLGLCRITPLPATRLIVFEALAVNVGSALTPIGNPQNLFL
                     WQLSQVSFAEFTIQMAPLVAVLSVLLAVLVALFFAATPLRIEDRSPSERLDRPLLLAA
                     LAMYVPFLVLTDRGEALWGCALAAGVIAAVRPRVLARIDWGLLLVFTLMFIDLRLVAG
                     LDAAQSAIGGLELADPVRLYWAGIGASQLISNVPAVIGLAEYSQDWRVMAYAANVGGF
                     GWMVGSLANLIALRMAQDSRAWISFHLCSVPFLAAAAAVGYGLLFTLNGPGA"
     gene            complement(17660..18520)
                     /locus_tag="PKV56_03450"
     CDS             complement(17660..18520)
                     /locus_tag="PKV56_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOX66897.1"
                     /translation="MKLMRHGPMGFEKPALVDARGVVRDLSGVLSDIVPATLSPHGLQ
                     RLRDIDPASLPAVEPGRIGVPYSGCAKFVGVGLNYADHAAESGLEIPAEPVLFMKATS
                     CLAGPNDAVQLPQGSVKTDWEVELGVVIGTRARYVSEHAALGHVAGYCIVNDISEREY
                     QLERGGTWDKGKSCDTFGPVGPWLVTTDEVPNPQALGLWLDVNGQRAQTGHSGAMVFG
                     VARLVSYISRFMTLNPGDLITTGTPPGVGLGRKPPSFLRAGDTMRLGIDGLGEQFQTV
                     HAWDASLAHG"
     gene            18688..19137
                     /locus_tag="PKV56_03455"
     CDS             18688..19137
                     /locus_tag="PKV56_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOX66898.1"
                     /translation="MAAAGQPASRQAKEGGARPARTPDSEKITINLGHVDLGQVDLLV
                     NEGFYGNRTDFIRTAIRNQLTQHSDVMKQVVARKMFVFGMQHISRHDLEQLELARQKL
                     TLRVVGLTTIASDVTPELALATIDTLEVLGALHASAEVKRALSGRLK"
     gene            19194..20369
                     /locus_tag="PKV56_03460"
     CDS             19194..20369
                     /locus_tag="PKV56_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOX66899.1"
                     /translation="MSSTLQRWMKEVTRLTRSGRLADATRAIQDALGRKPTMGSFNAP
                     PAEVNSQTAAAASAARLIPEPVPVETSKAIDDGTIRTGEYSEAGLTRSYLLYLPPTAS
                     AEHSGTPMPLVVMLHGCTQDPTDFAAGTGMNSLAREQGFAVLYPAQAQSANPSRCWNW
                     FKHNHQRRGSGEAAVIAGMTRSVVAEHHLDPCRVYVAGLSAGGAMAAILGRAYPDLYA
                     AVGIHSGLASGAANDLISALSAMKHGPASPTSPPPVTQSGEPAARDDGFSASIGPVPT
                     IVFHGDGDAVVHPGNGTQAIAVAIGPTGTGAPSAASPVVEVGESTKGRRYTRTTHPGP
                     AGAPAQAEHWLVHGGGHAWSGGREQGSYTDPSGPDATREMWRFFRAHSQPDAPRHEG"
     gene            20489..21358
                     /locus_tag="PKV56_03465"
     CDS             20489..21358
                     /locus_tag="PKV56_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOX66900.1"
                     /translation="MLAIIGGTGLYELPGMVIESHLDEPTPFGAPSGVISQGHIGDHR
                     VLFLARHGAGHRLLPHEVNYRANIYALKRAGATQILGFSAVGSLSEPIEPGHLAVPDQ
                     YFDWTRGGRQNTFFGNGVAAHVSTARPVSAALVDWVEEGVKRHAAHHAGSAPITLHRN
                     VTYACVEGPRLGTRAESLFLRSAGCQLVGMTNVPEVFLAREAQMGYATIGIVTDYDCW
                     MEDPSKHVTVSSIFALYRESIAVARALLETLLSGALPTPEADIRNALEHALMTSQDQL
                     TDAQREWLKILRL"
     gene            complement(21393..21686)
                     /locus_tag="PKV56_03470"
     CDS             complement(21393..21686)
                     /locus_tag="PKV56_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66901.1"
                     /translation="MQFNERVDGEYRVYAGALDAPRGEGYIAAVVVSRMLGTGVKARE
                     VFRDESLACGHRWQSADEALMFSMCKASEWIAADRQQAADLSSSLSGRREVQF"
     gene            complement(21848..24415)
                     /gene="cphA"
                     /locus_tag="PKV56_03475"
     CDS             complement(21848..24415)
                     /gene="cphA"
                     /locus_tag="PKV56_03475"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOX66902.1"
                     /translation="MKVSRVRALRGPNLWTRHTAIEAIVSCAAPECMLDHIAGFEARV
                     RARFPAIGVLPAASQQRPKSLAHVLEVAALALQAQAGCPVTFSRTSPTVDPGVYQVVV
                     EYSEEEVGRQALDLAQELISAALHDQAYDVEVAIARLRETHEDVRLGPSTGAIVDAAA
                     ARGIPWRRLTRGSLVQFGWGSRQRRIQAAEVDATSAVAEAIAQDKDLTKTLLLAAGVP
                     VPYGRPVSSLDEAWSVALEIGLPVVVKPQDGNQGKGVTVNIVNREHLEIAYRVAAEIG
                     DVMVERFLPGSDYRLLVVGDRMVAAARRDPPQVLGDGAHTVRQLVDLVNADPKRGDGH
                     STSLTKIRFDDIAVARLGEQGLTPESVPDKGRRVVLRNNANLSTGGSATDVTDSVHPE
                     MAARAVTAAQTVGLHICGVDVVCESVLRPMEEQGGGVVEVNAAPGLRMHLSPSYGKGR
                     PVGEAMIANLYVPGDDGRIPIVAVTGTNGKTTTARLIAHLFSTSGLRVGMTNTDGVYV
                     EGRQIDSGDCSGPKSARNVLMHPDVDAAVFETARGGVLREGLGFDRCQVAVVTNIGSG
                     DHLGLNYITTVEDLAVLKRVIVQNVATNGYAVLNATDANVVAMAANCPGDVIYFASDR
                     QHPVMVTHRAQGHRTVCVEGEWLVAAQGAWRERVALRDVPLTRNGAIRFQVENVMAAM
                     AAAWGTGLAWDAVRRGLASFSADSDNVPGRFNVLSFRGATVIADYGHNPDAMRALVAA
                     VDAMPAKRRSVVISGAGDRRDEDIREQTQILGDAFDDVLLFQDACQRGRADGEVLKLL
                     RDGLAGTKRCRRVDEIRGEFIAIDQALDRLEPGDLCLVLVDQVQEALAHLARRVTEA"
     gene            complement(24452..26746)
                     /gene="cphA"
                     /locus_tag="PKV56_03480"
     CDS             complement(24452..26746)
                     /gene="cphA"
                     /locus_tag="PKV56_03480"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOX66903.1"
                     /translation="MKRFDDIGLLRITYLRGPNIWTYRPALETWLDLGELEAHPSHLL
                     PGFSDRLIALLPALMEHHCGVGERGGFLERLESGTWAGHVLEHVVIELLNLAGMPTGF
                     GQTRSTSRRGIYRMVFRARDEHVARAALAAGHRLLMSTINGEPFDLPAAVEEVREKID
                     DCYFGPSTASIVAAATERGIPHIRLNAGNLVQLGYGAHQRRIWTAETEQTSAIAEGIA
                     SDKDLTKTLLRSCGVPVPEGQVVSSAEAAWDAAQDIGLPVVVKPTDGNHGRGVTLDLM
                     TREDVEAAYAVAEPEGSEVIVERYIRGHEHRLLVVGGRVVAAARGEAAWVTGDGRSSV
                     VELIDAQINSDPRRGLTEDHPLGRIDLGTDGAVVLDLQRQGLEPRAVPVSGRRVLIQR
                     NGNVSIDCTDDVHPEVAHQVSLAARVVGLDIAGVDVVAEDISKPLHLQGGAIVEVNAG
                     PGLLMHLKPAEGMPRPVGQAIVDHLFPQEDDGRIDIVGIAGSIGSHTMARLVAWLLHL
                     SGRRVGLACRDGLFLGSRQIDRSDGARWEAGHRLLLNRSVDAVVIENGAPSVLDDGLA
                     YDRCHVGVVTDLALNDNDAAALAAHDVHDSDQLVKVLRTQVDVVLASGVAVLHAADPR
                     IADMASLCDGEVLLYARDETNEALSAHQAAGGRIAFVRDGWAVLAKGAVETRCTNVLY
                     AARRFVAPAHPAMPPADPVDVLLASVAAAWAMGISPELMAAGIETFPVVIAAGVVVSP
                     GSAAASPSVAAAAVPTGAAAIASA"
     gene            26845..29220
                     /locus_tag="PKV56_03485"
     CDS             26845..29220
                     /locus_tag="PKV56_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX66904.1"
                     /translation="MQHLHPVQDPSHRPASVAKSQPVPLESDENALGSLLVDLSGNLR
                     FVSGELILTNRRLLARVDDGNEAPGSETAWVSWRLDEPAAVRLRLLHTDHGGIGALEL
                     HDDLGRLAIWRFTLAQNILALRLVTLFDAHQARRPWRAGADELGTTVDEPALCPSCRA
                     PLPPDSDDCPVCSREVQAVPSTWVLLRLWRFAKPYRKPLAGGFALTLASTAATLVPPY
                     LAIPLMDDILIPFQNGKQIDPMHVLIYLGGLLLAALLAWGLGWARTYLLALVSERIGA
                     DLRTATFEHLLQLSLDYFGGKRTGDLMARIGSETDRICVFLSLHALDFATDVLMIVMT
                     AVILFSINPWLAVVTLLPLPFIGWMIHTVRDRLRTGFEKIDRVWSEVTNVLADTIPGI
                     RVVKAFAQEGREAERFRVANQSNLLVNDRLNKTWSLFTPTVSLLTEIGLLVVWAFGIW
                     QVSRNEITVGVLTAFIAYIGRFYGRLDSMSRIVSVTQKAAAGAKRIFDILDHVSNVPE
                     PATPLPLTNPQGRIELNGIGFRYGNRSVIRGLDLEIRPGEMIGLVGHSGSGKSTLVNL
                     ICRFYDVTDGAIKVDGIDIRRYGVADYRRHIGLVLQEPFLFFGTIAENIAYGRPDASR
                     QDIVAAARAAHAHEFILRLPQGYDSLVGERGQGLSGGERQRISIARALLIDPRILILD
                     EATSSVDTETEKEIQNALDNLVQGRTTIAIAHRLSTLRKADRLVVMDRGQIAEVGPHD
                     DLIARRGAYWRLHEAQLRQAAADDWSPVSDGVDAPVAAVAAGPSSHSELPA"
     gene            29217..29687
                     /locus_tag="PKV56_03490"
     CDS             29217..29687
                     /locus_tag="PKV56_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOX66905.1"
                     /translation="MSGEPFQLERNAHGRLVLITSGGESHAGVVPIRAFPIAAPQEGL
                     SLVDADGREVAWVDRLDQLAEPLRALIEEELASREFTPEILRIRSVSTYSTPSIWEVD
                     TDRGPTRFVLKGEEDIRRLKDGALWIADSHGIQFVLHNRKALDRRSRRLLERFL"
     gene            complement(29699..31804)
                     /locus_tag="PKV56_03495"
     CDS             complement(29699..31804)
                     /locus_tag="PKV56_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX66906.1"
                     /translation="MSPLIEGLLDAAWLVDPDSLRILSANAAAATLLGMDLQALCRAD
                     ALELAATPEDVVFWESGVADQADSTDSQTFVSRADGKLVPVLRRVTRIPFGSGHAAAP
                     GGTNETYLVVMRDRSLELRTQAELEERLSQLRATLESTTDGILVTDLTGRIVNFNPRF
                     VALWGVPEPLLAEHDDAAVSAWMLRRVSDPVGYTRRLQSLDESMTLEGSDVLTLRCGK
                     VLERRTLPQRNRGQPIGRVFSFRDISESLEANRRIDAMSSTDALTGLPNRRVVASRIE
                     YALALAQREGVAFALLHLNLDRFKPVNDALGSAAGDRVLVEVARRLTDTMRQVDAVAR
                     LGADEFVMLVHQADMQRAEGAARRLTEALQAPFVIDGVNLTLTASIGIALFPGDGATP
                     DDLLQAADAAMTELKSAGGAGYRFHHQRAGATVVALRTRVKLDHAMRHALASGRFRLH
                     YQPQADLDSGEIVGAEALIRWRDPELGDVPPTEFIPVAERSGFIVSIGAWVLRQAVQQ
                     AALWHGRGHRLLMSINVSALQFQQPDFVDSVAQALAEVGLDPQWLELEMTESILIQDA
                     QEALHRLQALARLGVKLAIDDFGTGYSSLAYLKRFPIGRLKIDRSFIQGLPADEVDVG
                     IVKAIINLGNALHLEMVAEGVETEAQRLFLRNIGIRQYQGSLCSPAVDALSFDALLDR
                     GLAFAQDLPDEGGWIGGGS"
     gene            complement(31921..33198)
                     /locus_tag="PKV56_03500"
     CDS             complement(31921..33198)
                     /locus_tag="PKV56_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOX66907.1"
                     /translation="MQITQSSEPIDVSALRLGMFIHLEGGWLSHPFPRSSFKITSEDQ
                     LVTLRSLGISVLRWSPELSDPEVLPASAGAAQEVTAPEVDPAEAAALEARAARRAALS
                     AQRQALAACERRFNEAARATKVVFDTVVSQPAQARQGAQALTRELVDKMIGSEELCIR
                     LLSEAAGDKASAHAINVSIISLLMGRTFGLSETEMLDLGLGALLHDIGKGDIPERFRH
                     RDEHHTTAEQKLYETHVVHGIAQARRMELGVGASTVIAQHHELIDGSGFPLKLTSDRT
                     TVPARIVALVNRYDNLCNPANPGKAVTPHEAVALMFAQGKNKYDPAILGAFIKMMGVY
                     PAGSVVQLTDDRYALVVGVNSSRPLKPRVLVHETGVPREEALILDLETAGGLGIRRSL
                     KPTALPEQTLTYLSPRPRISYFFESVGDLELVS"
     gene            complement(33268..35313)
                     /locus_tag="PKV56_03505"
     CDS             complement(33268..35313)
                     /locus_tag="PKV56_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66908.1"
                     /translation="MSHPTTMHPTARLSRSTEPPSSTTDLRESAVRGLRRRDDRDPIL
                     EMQSLLDKLPGMVVRLCRSADGRWSVPYASTAFIGLCGIGLPALRNDARPLAACVHPE
                     DLRRLQCLGAQENEPPLPIKETLRIRRPNGQWAKLDASAMVDRSVDGGLYLNVLLNEA
                     ALPPAVDLELQRQHNRWQRAIAAADIGMLEISLLAGSLQFDEVACRHLGIDPAESRWA
                     FDSWLERVAEADRTAARALLAKPSAPDEVTRLLLSFDLADGQGPRTLEFVLQAVADDQ
                     RRVGLCTDVTQRKTLDALKRDKLDAEKANRSKSEFMSRVSHELRTPLNGILGFAQLMA
                     LDAEHPLAPAQAQRLEVLRQSGSRLLSLIDQLLEISRIEQGRLRLRNRPIDVRLLVER
                     CAVEVMPLAQQRGIELLVQIPLTAGPVRADPDALEQVVTNLLTNAIKYNRPKGRVRVR
                     FEAGEQGVLTVDDTGIGMTEEQLDKLFEPFNRLGAEKTETQGSGLGLVITRKLVRAMG
                     GKIDVLSRPGRGSRFVLSLPLAKTGRPKAPDVRSPVAVPSTWDNGVEQVVLYVEDDEV
                     NTILMEQIFRSQPNWRLLTAATGPEGLEIALENELSLVLLDLNLPGLSGFEVMERLKA
                     DEQTRHLRCIALSADALPHQIQHALSMGFDDYWTKPIDVATVIGKLKDEFRQLSEAV"
     gene            35575..36012
                     /locus_tag="PKV56_03510"
     CDS             35575..36012
                     /locus_tag="PKV56_03510"
                     /inference="COORDINATES: protein motif:HMM:NF027482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HOX66909.1"
                     /translation="MNAALIQRRALITLSLGLATGVAWPAEVPEAQAAKAREVVQAQL
                     DAFAADDARRAFLLASPSARRHLGSPDNFINLVRRNYAVVYRPASVAFLKPQLIDGVV
                     ILGVQMTDAQGSAWLATYQLERQPDGIFLIDGCELVANEGRYT"
     gene            36084..37475
                     /locus_tag="PKV56_03515"
     CDS             36084..37475
                     /locus_tag="PKV56_03515"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOX66910.1"
                     /translation="MAQPRELSAVELVRAYRERRLSPMEVAHDVLRHIDAWEPSLHAT
                     YALDADATLAQARAAEARWLQGTPLSALDGVPSLIKENVATRGVPVPLGTAATELLPA
                     TDDAPPSARLREAGALIMGKTTMPDLGMLSSGLSSLHPLTRNPWDLRLNPGGSSAGAA
                     AGAAAGYGPLHVGTDIGGSIRLPAGWCGVFGLKPSHGRVPIGAPYPARVAGPITRSVT
                     DSALMMSVLSQPDWRDHMSLPPERMDWLQLDRDLRGLRIGLMLDAGWGLATDPEVRAA
                     VVAAAQAFESAGAIVEPLKPFATREMADGIDRFWRIRAWVDLQAFSAERQQRVLPFIF
                     EWARGGAGLSGTDVMKGYTQTLAMRAATVAACQPFDYVLSPVAPMTAFPAELPCPTND
                     AARPFEHIAYTLAFSMSEQPAASINAGYSESGMPIGLQIVGRRFDDLGVLQLSRAWEQ
                     MRAAQRPWPAAPG"
     gene            37656..38069
                     /locus_tag="PKV56_03520"
     CDS             37656..38069
                     /locus_tag="PKV56_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66911.1"
                     /translation="MEDLSTTLNRLTMEQHLLAGVFLLGYGLALGSMFSPKGRLRAAL
                     LALLAAIAFSARTHPWEHGVLLVGFAIGGLAVFIALASAFSRLGLRHEATVGPMSASS
                     SAAGRLSDKLRGTARHIAPAAGLAARRRRRRARSV"
     gene            38301..38753
                     /locus_tag="PKV56_03525"
     CDS             38301..38753
                     /locus_tag="PKV56_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66912.1"
                     /translation="MHERARRNLLLLIDGVRPQAALLDSVSGIGPDDFEQLARLGLIA
                     RAPAGSTPPPRPAVTVAPVSRPTSLPDRDPGTGEHLPTQNYGQLTTKLTQLISSELGL
                     RGFLLTLAVEKAADIKDLASVTERVLAEIAKRKGDRVAETARRSLFGA"
     gene            38912..40510
                     /gene="dsbD"
                     /locus_tag="PKV56_03530"
     CDS             38912..40510
                     /gene="dsbD"
                     /locus_tag="PKV56_03530"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HOX66913.1"
                     /translation="MTTALLRFLVSILLALQAGLITPAAAAQDFLPPEQAFRFEARAV
                     DARTVEIVFSVADGYYLYRDRFAFAADGLDVRLGDAHIPAGKVKFDETFGKDVETHRG
                     RVVIRVPVESTPESVALTVTSQGCADAGLCYPPMQSNVRMQLAGLGAGLSAGLGDEIR
                     AAFDGTPGLDSALRSGHLWIVAGVFFVAGVLLSLTPCVLPMLPILSSIIVGQSGSSRP
                     TTRSRGLGLAMTYSLGMALVYTALGVAAGLAGEGLAASLQNPWVLGAFGAVLVGLALS
                     MFGVYELQLPAALRDRLDARASGLKGGRWAGVFAMGGLSALIVSPCVAAPLAGALLFI
                     SQSRDVMLGGGALFSLAVGMSVPLLLVGASAGALLPRAGAWMDWIKRFFGVLLLGVAY
                     WIVSPVLPTFGAGTSATAPADAEIHFKRIRNVGELEAALRSAARPVMLDFYADWCVSC
                     KEMERYTFVDPRVRAKLDQVLLLQVDVTQNTSEDKELLKRFNLFGPPGIVFFDAQGTE
                     VPNRRVIGYQNADRFLESLAQAGL"
     gene            complement(40514..>40850)
                     /locus_tag="PKV56_03535"
     CDS             complement(40514..>40850)
                     /locus_tag="PKV56_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOX66914.1"
                     /translation="LLIAGVFLYRGLRFVTGTPINHWPRGAKPADDAPLVKRLAAAGL
                     VHRERDPANERNLRVQLTAAGRALHAPAALVQQRVACQTGLRQEEFLLLRSQLHELAR
                     RLEQAQAET"
BASE COUNT         7350 a        13459 c        12957 g         7084 t
ORIGIN      
        1 cgccgcgcga ggcctacctg aactacctgg ccgcgctgcg cggcctgggc ttcaccgtgg
       61 tggaagtggg cggcctgttc aaggtcgttc cggaggccga tgccaagctg caatcgggca
      121 ccgtctcggt gggcgaggtc agtcggcgag gcgatcaggt gatcacgcag atctataggc
      181 tgacgcacga gaatgcaaac aacctcgtcg cggtgctgcg gccgctgatc agccccaaca
      241 acaccatcaa cgccaacccg ggtaacaaca cgctggtcgt caccgactat gccgacaacc
      301 tgcagcgcat cggcaagatc atcgcggcga tggacacccc ggctgctggc gacgtcgagg
      361 tgatcacgct gcagcacgcc gtggcgagcg atatcgctgc gctggtccag cgactgaccg
      421 acaccgcgac aggcgcggtg cagggagcaa cggtggcttc cagcgcctcg gtcctggtgg
      481 atgcgcgcag caattccctg ctggtgcgcg cgccgaaccc ggcacgtctc gcggccatcc
      541 gcgcgctcgt cgacaaactc gaccagccga tcaagggcgg aaacgcggcc gggaacatct
      601 gggtggttta cctgaagaac gccgatgcag tgaagcttgc tgcggtgctt cgagcggcct
      661 atgcgggggg ctccggcacc tcctcgtcag gcggcagctc gagcacgccg gcggctccgc
      721 aatcatcgcc caacaccctc ggcaccagca ccagcaccag cagcgggagc acttccagcg
      781 cgtcggcgca ggccactgca cccgtgacgc agtcggccgg accctcgacc ggaggttttg
      841 ttcaggccga tccggcgacc aactctttga tcatcaccgc accggagccc atgtatcggc
      901 aactgcggtc gatgatcgac cagctcgact cgcgccgtgc tcaggtgtac atcgaaagca
      961 tgatcgtcga ggtcagcggt gacgactcgg ccgatttcgg gttccagtgg caatccatcg
     1021 gcaacagcaa cgacaagtac atcggtggca tcggcaccaa tttcggcacc gggggcacca
     1081 acatactgac gaaccaggga tcaacgaccg cggtcggtgc cctgaagggc ctcgggcaag
     1141 ggttcaatgt cggcgtggtc cgcaacttcg gcggcacctt cgggctgtcg gcagtggcac
     1201 gagccctgca atcgcgggca gacaccaaca tcgtgtcgac gccgaacctg atcacgctcg
     1261 acaacgagga agccaagatc gtggtcggca gcaacgtgcc gttcatcacc ggccagtaca
     1321 ccaacacggg tggcaattcg aacaacagcc cgttccagac catcgagcgc aaggacgtcg
     1381 gaatcacgct gcgcatccgc ccgcagattg gcgagaacgg caccgtgcgc atgaccctct
     1441 tccaggagtc gtcgagtctg gcggccggca cggctccggg cacgtcgagc gcggggccga
     1501 ccacgaacaa gaagtcgatt gaatccacgg tggtcgtcga cgacgggcag atcatcgtgc
     1561 tgggcggcct gatcgaggac tcgatcgtgg tggacaagtc caaggtgccg atcctcggcg
     1621 acatccccta cttcggcgcc ctgttccgca gcgagacccg aaccaagaag cgcaccaacc
     1681 agatggtgtt cctgcgcccg gtcgtgatgc gcgatgcgtc gtcgtcgaac aagctgtcgg
     1741 tcgatcgcta cgacctgatc cgcggtgaac agaaggacgt ccagttaccg accagcccga
     1801 tgatgccgat cggtggcgtg ccggtgattc cgccgctgaa gccgaccgag gatccgtcac
     1861 agccgctgac gccacccgcc aaccgcccgc cgccctcggg cctcgtgcct ccaacggcca
     1921 cgcccgatgg cgcggcgacg gccaactgaa gccggcgctc gatcatggga acccgccatc
     1981 cgctgcctta tgcctacgcg cgggccaaca ccgtgctgct cgaagacgac ggtgaccggc
     2041 tggtgctgtg ggcgcccgag aacgtatcgc tgacggccct cggcgaggtg ctgcgacggt
     2101 ttgacgtcga tgcgctggag cgtgaatcgt cgtcgaccct ggcgaaccgc atcgcagtgg
     2161 cgtatgccgg aagcgaatcg agcgcagcca cggtgatcgg cgaggtcgaa agcgcggtcg
     2221 acctgagccg catgatgcag gaactgcctg ccatcgagga cctgctcgaa gcatcgaacg
     2281 acgcgccgat catccgcatg ctgaacgcac tgctgaccca ggcagcgaag gacggcgcga
     2341 gtgacatcca catcgaaccc tacgaacgca gcagttcagt acgctttcgt gtcgacggca
     2401 cgctgcgcga ggtggtgcag cccaacaagg cactgcacgc cgcgctgatc tcgcggctga
     2461 agatcatggc cgagctcgac atctccgaga agcgtctgcc gcaggacggc cgcatctcgc
     2521 tgcgcatcgg cgggcgcgcc gtcgatgtcc gggtcagcac gttgccgtcg tcgcatggcg
     2581 agcgtgcagt gctgcgcttg ctggacaagg gcgaaacaag gttcacgctc gagagcctgg
     2641 gcatgagcgg cgatgtgttg acgagcttcg accgactggt acagcagccg cacggcatcg
     2701 tgctggtgac ggggcccacc ggctccggca agacgacgac gctctacgcg tcgctcggcc
     2761 gcatcgacac atcgacgacc aatgtgctga cggtcgagga cccggtcgaa tacgaactcg
     2821 gcggcatcgg ccagacgcag gtcaatccga agatagacct gacgttcgcg aaagcgctgc
     2881 gggcgatcct gcgccaggac ccggacgtga tcatgatcgg agagatccgc gattacgaga
     2941 ccgcgcagat cgcgatccag gcctcgctga ccggacacct ggtgctggcc accctgcaca
     3001 ccaacgacgc accgagcgcg gtcacccgat tgaccgacat gggcgtcgag ccgtttctgc
     3061 tcagctccag cctgctcggc gtgctggccc agcggctggt gcgcaagctc tgcgtgagct
     3121 gcaggaaggt cgatgcgcag gggcgctacc acccggtcgg ctgcgccgat tgcgggcaga
     3181 ccggctacaa gggccgaacg ggcgtgtacg aaatgatggt tgccgacgac gcggtgcgat
     3241 ccctgatcca cagccgggct gccgagtcgc agttgctggt cgctgccgag catgccggtc
     3301 tgcgttccat gcgagaggat ggcgaacgcc tggtgatcga aggtgtcacc tcgctggaag
     3361 aggtgttgcg cgtgacccgc gaatgacatg cctgcgtaca agttcgaagc gctgaccgcg
     3421 gccggaaaat cgaccaccgg gctggtggaa gccgacaacg cgcgcgcggc gcgcgcgcaa
     3481 ttgcgcgcgc agtccctggt gccactggac gtcacgcaag tcgcctcggc cgacgccaag
     3541 ggtcagggcc tgcgcttccg gcgaagagtc ttctcgacca ccggattggc ggtctggaca
     3601 cgccagctgg ccggtctggt cggtagtggc ctgccgctgg aacgggcgct gaccgcgctg
     3661 agcgacgagg ccgaagacgc accgcagcgc gaactggtcg cgcacctgcg ttccgaggtc
     3721 aacgccggat cgccgttcgc acgcgccctg gccagtgccg agcgtgaatt cgacgatgtc
     3781 tatcggggcg tggttgcagc cggagagcaa agtggcgcgc tgggtcaggt gctcgaacgc
     3841 ctggccgacg acctggaaga acgccaggcg ctcaaggcca agctgatcgg cgccaccctc
     3901 taccccgcga tcgtttcggt gatcgcggtc gtgatcgtga tcttcctggt cacctacgtg
     3961 gtgccgcagg tggcctcggt gttcaccagt tcgaagcgcg cgctgcccgg actgaccgtc
     4021 gcgatgcttg cggtcagcgg cttcctgcgc agctatggat ggttgctgct gctggcgatc
     4081 ggtggcggga ccgccacgct gatgctgatg ctgcgaaatc cgtcattccg ggagcgcttc
     4141 gatgccgcgt ggctcaacct gccactggtc gggcgcctcg cacgcggcta caacgccgcc
     4201 cgtttcgccg gcacgctggc catgctggca ggagccggcg tgccgatcct gaaggctctg
     4261 caggctgcgg cggagacgct gtcgaaccag gcaatgcgag ccgacgcgct cgacgccctg
     4321 gtgcgagtgc gtgagggggc accgctggcc tcggcgctgg ccggaaagaa gcgattcccg
     4381 ggactgctgg cgatgttcgc ccgccttggc gagcagaccg gccaactgcc gcagatgctg
     4441 gatcgggcag ccaagcagct cgccactgaa gtccagaggc gcgcgatgca gatggcgacc
     4501 atcctcgagc cgctgctgat cgtcgccatg gggggcgtcg tgatgctgat cgtactggcc
     4561 gtcctgctgc cgatcatcca gctgaacacc tgggtgaaat agagcccccc agtcgcttcg
     4621 ctcctgcccc caaggggcgc cgccctgcgg accggcaaag ccggttccgc tggcgtggct
     4681 tgatcaggcg cgtcgcttcg cttgctttct cgatctgatg gcctgcggca tgcatggcga
     4741 ttgacgtgtc aatgcggtgc ttctccgaag tgcgtggcgc actgcacgtg gttgaatgat
     4801 cccagcgagc tttgctatcc ggaggtgaga tgtctgcttc ccatgaactg gttcatgtgt
     4861 ctgccactcg cggcggcatc ccgattgcgc gaatgcgcag cctctaccgc gtcgacgacg
     4921 ttggtcgcaa gctcgacaag cttccacaca aggaacacga gaacctgcgc tccacctacg
     4981 aacgcatgat cgaactggga cccgaacgct ttcaggtcaa gccgtccggt ctgccggtga
     5041 tggatcacct gtacgacgac ctgcccaact tctgcgaggt gctcgacgac gtgaagcgcc
     5101 agcttgcgct gtgcgaggac agccgcgacg cgctggagat cacaccgctg ctgttgctcg
     5161 gcccgccggg tgtcggcaag acacatttcg cgcgcgaact gtcgcaactg ctgggcaccg
     5221 gcatgggctt catctcgatg agctcgatga ccgccggctg ggtgctgtca ggcgcgtcca
     5281 gccaatggaa gggcgcgcgc cccggcaagg tgttcgagac tttggtggat ggtcagtacg
     5341 ccaatcccgt gatggtggtc gacgaaatcg acaaggccgg cggggagcac gcctacgatc
     5401 cgctgggagc gctttacagc ttgctggagc acgacaccgc aggcagcttc atcgacgaat
     5461 tcgccgaggt cccgatcgac gcaagtcagg tcatctgggt ggcaacagcc aacgatgcgc
     5521 gggggattcc cgatccgatc ctgaacagga tgaacgtcta cgaagtgcag gcacccgatc
     5581 gcgaggccgc gaggcacatc gcggccaagc tgtattcatc gatacgcgcc agccacgact
     5641 ggggcagccg attcgacgaa cagccgagca gcgacgttct ggaccgcctc tgtgaaatgg
     5701 ctccgcgcga gatgcgtcgt gcctggatga ccgcattcgg caacgccaag ctggaacgca
     5761 gatcgcaagt ctcgacggcc gacctgccgg acgctcacca gcggcgcgca cccatgggct
     5821 tcgtgcagta gccgtcgcag cgtccggaac gcagcgacgg cgtgggcttg cccccgtatc
     5881 atcgatcgca tgcctgtctc actggatggc caactggtcg tcgccatctc gtcccgcgcg
     5941 ctgttcgatt tcgaagaaga aaacgaagtc ttcgaagcgt cggacgacag ggcctacatg
     6001 cggctgcaac tcgagcggct cgatgtaacg gccaagcccg gcgtggcatt ctctctggtc
     6061 aacaagctgc tggcgttcaa tcgcgccccg gcatccgaag ccggagaagc gtcgccaacg
     6121 cagcgagtcg aagtggtcat cctgtcgcgc aatgacccgg tgtcgggcat gcgtgtattc
     6181 cggtcgtcca aacagcatgg attgccgatc cagaggggcg tcttcacgcg gggtcatccg
     6241 ccatggcgct acctgcaacc gctgaacgcc aacctgttcc tgtcgaccaa cgaagccgat
     6301 gtgcgctcgg cgctggacgc gggcacgcca gcggcccgcg tcttcccgaa ttcggcgcga
     6361 gcctcggcag cgcatccgaa cgaagtgcgt atcgctttcg acggcgacgc ggtgctgttc
     6421 tcggacgaag ccgagcaggt attccagcga gcaggcctga acgcgtttca ggaacacgaa
     6481 cgcgaccgtg ccgagaagcc actggccgcg ggacccttca agcccttgct cgaagcgctg
     6541 cagcggctgc agcgggcggc aacgccatcg atgagcatac gcaccgcact ggtgaccgct
     6601 cgcagcgccc ccgcccatga gcgcgcgatc cgcacgttga tggactggga catcgagatc
     6661 gacgaagcca tgtttctcgg tggcctggcc aagggcgagt tcctgcgcga gttcgagccc
     6721 gacttcttct tcgacgacca gacaggccac atagaaaacg cggcacctca cgtgccggca
     6781 ggtctggtgg cctcgggggt tctgaaccgc tgaagcctgc tgggtcagct cagcgtcacg
     6841 cgcgcgaact tgcgcttgcc gacctgcaac acgtaagtgc cagcggacaa cttcaggcca
     6901 cgatcgctga ccacattgcc gtcgacgcga acaccgccgc catcaacgag gcgcagcgct
     6961 tcgctgctcg acggcgccag catggactcg cgcagcaggg cccctatgcc gagcggggca
     7021 cccgtgacag ccaactcagg gatcacatcg ggaatacctc cgcgcgcgcg gaggttgaag
     7081 tcgttctcgg ccgcatcggc cgcagcagcg ctgtggaaac gggccgtgat ttccttggcc
     7141 agcaacacct tggcgtcctt cggattccga ccgccctcga ctgcgttcct gaggcgtgca
     7201 atctcctctt ccgacttgaa actgagcagg gtgtagtagc gccacatcag cgtgtcgctg
     7261 atcgacagca gtttggcgaa catgtcattc ggcgcttcgc tgatgccgat ggtgttgccc
     7321 ttgctctttg acatcttctc gacgccgtcc aggccttcga gaagcggcat cgtcaggatg
     7381 cactgcggat cctggccgta ttcgctttgc agggcccgac caaccaacag gttgaatttc
     7441 tggtcggtgc caccgagctc aagatcgctt ttcagcgcga ccgagtcata gccctgcatc
     7501 agaggataaa gaaattcatg cacgctgatc ggcgttcccg ccttgaaccg cttggtgaag
     7561 tcatcgcgct ccatcatgcg ggccacggtg tagcgggaag ccagttggat caacccgcga
     7621 gcacccagcg gatcgcacca ttctgagttg taacggacct cggtcctttc ggggtcaagc
     7681 accaatgagg cctgctggta gtaggtcttc gcgttgtgct cgatctgctc gcgcgtcagt
     7741 ggcgggcggg tggcattgcg gccggacggg tcgccgatca tcgaggtgaa atcgccgatc
     7801 aggaagatca ccgtgtggcc cagctcctgt agttgcctga gcttgttcag caccacggtg
     7861 tgaccgatat ggatatccgg cgcagtcggg tccagaccca gcttgatacg cagtggcacg
     7921 ccagttgctt ccgccctggc cagcttgctc agccagtcgg cttccggtaa cagttcgtcg
     7981 cagcctcgac gggtcgcacg aaatgcgtcc cgaacccgat ccgtgaccgg gtaggaagtt
     8041 gcggcgctga cgctgggttc taggcggtcg gtgtgcgaca tgtcgggaaa ctactgaaca
     8101 ttggatggat gagaagctcg tttctcatga caaattcgcg aaagcccgtc ggtatacttc
     8161 tcgatccggt tgcagccggc tagcaacacg aagaactcga agcaggcagt ccacggcccc
     8221 tgtccgaatc gccccgaagt ctaagggacg gcaagcgcag agcgagattc cggtccggcc
     8281 acgcgcggga cgacgtcaac agataaccag agcgaacggc tccccgaatt ttggcttcat
     8341 ctgattttct cgatcgcatc gctgttcatt tggggcaacg caccggggat ctgttgcgca
     8401 agcacccccg acagctttat tcggcgatcg ttctggcctt ggccggattc ggcgccacgg
     8461 ccttcggcgt ggctccactc agtgcggaca acagtggcat cgcgcagagc tgggtgacgg
     8521 aatcggtcat cccgagcgat ttggcaggac aagtcctgtc gctgtcccaa caggatctgt
     8581 cacttgtccg caacgacgtc actcgccccg gtgacacggc agacagtctg ctgcggcgac
     8641 tgaacgtcga tgacgcccag gccctggttt ttctgcgtgc caatcgtgtt gcacgcgaac
     8701 tggtgtcagg tcgtggtgga aagatggtgc aggtacgcac gagtggttca ggcgaactgg
     8761 acgaactggt cgcacgttac ccggcagcca gttccgcaca gtcgaacacc catttcacac
     8821 gtttgcgtgt gacccgggaa ggcgagcagt tcgcggctcg actcgaaaca gtgcccctcg
     8881 cctcgcagac ccgaatgggc agcggcgtcg ttcgaaactc actgttcaac gccaccgacg
     8941 aagcaggcat cccggactcg gtcgcaactc agcttgcgga agtgtttgct accgacatca
     9001 actttcaccg cgatctgcga cgcggcgaca ctttctcggt ggtgtacgaa gccttgacgg
     9061 ctgacgggga gccgatcacc tggaacgact ctgcaggccg cgtattggca gctgagttcg
     9121 tgaacaaggg aaaagtcaac tctgccatct ggtacgagag caccgaggcc gggcgccgcg
     9181 gagcctactt cgatctcgaa ggacgaagca agcagcgctc gttcctcgcg agcccgatgc
     9241 agttttcgcg catcacatcg ggttttgcga tgcgcttcca tccgatcctc cagaactggc
     9301 gtcagcacgc aggggtcgat tacggcgccc ccgtcggtac gccggtccgc actgtcagcg
     9361 atggcgttgt cgaattcgcc ggctggcaaa acggatatgg caacgtggtg gtcgtgaagc
     9421 acgcggccga tcgcagcacg ctttacgcgc acctgagccg gatcgatgtc cgaaagggtc
     9481 agcgcgtgga acagggcacg tacctcggtg cggtgggtgc gactggttgg gcgacgggac
     9541 ctcatctgca cttcgaattc aaggtgggcg gtgcacagcg agatcctgcg gcgatcgcga
     9601 gtctttcgga aacggtcgcg ctcgctccag catctcgggc ccagttcgca acgtgggcac
     9661 aatctgccaa agcccagttg agcgcggccg agaccatggc gcaggcaagc atcagcttcg
     9721 agtgacgaga catcttggac agctttccgg ctgatgcaga acgagcccgc agcagcgggc
     9781 tttttcttgt ttgaatgcag cgcaccgtgg ctgatctgtt catcgggttg atgtcgggta
     9841 catccctgga tggggttgat ggcgtattgg tcgaattcgg cagtaatgac gagattctct
     9901 cgatcgccgt acgagcccac gtgcaccgcc cattcccgct ggagttcgcg cgcgagctgc
     9961 tttcgctcaa cgtaactggt gcagacgagt tgcatcgtgc agcactggcg ggcaacgcat
    10021 tggccggcca ctatgcagcc gtggtgcacg acctgctaaa gaaggcatcg atcgcagcgt
    10081 cagaagtgac cgccatcggc gcacatggcc agactgttcg acatcgtccg ggtgagttcg
    10141 acggtatcgg ctacacagtc cagctcaaca acccggccct gctggcggaa agaacgggga
    10201 tagacgtggc ggcagacttc cggtcacgcg acgtcgctgc cggcggtcaa ggggctcccc
    10261 tggtgccagc gtttcaccga gcggccttcg gcaaagcgca cgaaaccctc acggtgctca
    10321 atatcggcgg catcagcaac ctttccgtgc tggcgtcgga tggctccacc ctgggctttg
    10381 actgcggtcc cggcaatgcg ctgatggatc actggtgtgc actccacctc ggcacagcgt
    10441 acgaccggga tggcaattgg gcctcttccg gttcggtcaa tccaaccctg ctgacggcca
    10501 tgcaaggcga cccctacttc gcccggcccg cacccaagag cacaggccgc gatcaattca
    10561 atgcggactg gcttgatcga tgcctccagc aaaacggagg cagcgcggcc ttggctccgc
    10621 aggacgtgca agccagcctc gcggagctga cggcctggtg ctgcgagcag tcggtgctgc
    10681 ttcagtctcc cgaagcgact cgactgctgg tctgcggcgg aggcgcgtgc aatacccatc
    10741 tgatgcgccg cctggccgcc cgactttcat ggctcgacgt ccagaccact gctgagcggg
    10801 ggctgccggt ggatcaggta gaggcagtgg ccttcgcctg gcttgcgcag gcttgcgtaa
    10861 aacgccgacc gggcaacata ccggccgcga ccggtgcagc tggcagccgc gtgctcggcg
    10921 cgatataccc ggcggagtga ccggagagta gagaagcacc aagaaaaaag ccgccccagg
    10981 gcggcttttc gagtgtgtga aatgtagggg gagtcacccc gccgaatcaa accgagaagc
    11041 tggacccaca gccacaggtc gtcgtcgcat tcggattcct gatcacgaac tgcgcaccct
    11101 gaaggtcgtc cttgtaatcg atctccgcgc ccaccaggta ctgcaggctc atcgcatcga
    11161 tcagcagggt gacgccattc ttgtccatcc gggtgtcgtc ctcgttgaga acctcgtcga
    11221 aagtgaaccc gtactgaaaa ccggaacaac ctccaccttg aacgaaaacc ctcaacttca
    11281 gatcggggtt gccttcttca tcaacgagtt ccttcacctt ggctgcggca ctgtcagtga
    11341 acaccagggg cgcgggaggc tcgtcctggg ttgctgctag gtgagtgagt tccgaggctg
    11401 cattcatttc tggatcctga gtagaagcgt ctgatattcc aattatggcg gcgatagccg
    11461 gaaaacgaaa aagccgctcg tgagcggcct tttcgcatgc agggcattga ggtcagcgct
    11521 tgctgaactg cttgcgtcga cgtgcaccgt ggaaaccgac ctttttccgc tcgacttccc
    11581 gagcgtcacg ggtcacgaac ccagcgcggc tgagttccgg cttcagggcc gcgtcgtaat
    11641 cgatcaatgc gcgggtgatg ccatgacgca ccgcaccggc ctgacccgat tcgccgccgc
    11701 catgcacatt caccttgaca tcgaaagccg cgtcgtttgc cgtcagcatc agaggctgct
    11761 tgacgatcat gatggatgtc tgccgaccga aatactcgtc gacggtcttg ccgttgacga
    11821 ggatctgtcc cgtgcccttc ttcatgaaga cgcgcgcgac ggacgacttg cgacggcctg
    11881 ttccgtagtt ccagttacca atcatcagtg tccccttaga tttcgagcgg cttgggctgc
    11941 tgcgcggcgt gcggatgcgt accgcccgcg tacaccttca gcttcttgac catggcatag
    12001 cccagtggac ccttgggcaa catgcccttg acggccttct cgagcgcccg cccgggatgc
    12061 ttggcctgca tgtccttgaa cttcgtcgcg atgatgccgc cggggtagcc ggtgtgacgg
    12121 tagtacacct tgtcattggc cttgttgccg gtgacgcgga gcttgtctgc attgacgatg
    12181 acgatgaaat cgcctgtgtc gacgtgaggc gtgtagatgg ccttgtgctt tccgcgcaaa
    12241 cggagggcga cttcactggc cacccgtccg agaaccttgt cggtcgcgtc aatcacaaac
    12301 cactcatgcg tcacctcggc cggcttggca ctgaaggtct tcatggttta tccaatacga
    12361 gaaatgagcc gaatgtggat cagcgaggaa cttcgctgaa ccggatcgcc gttggaaccg
    12421 cttcttgtga tggcggcctc tcgggcgatt ctttcgtgcc gcgccgggaa ctctcgctcc
    12481 ggatcacgca cgccgacctg aaggcttgat actcaggtca gcccgcgagt gtagcagacg
    12541 gctgggttgc cggtgtgcag cccccaacag gctcgcaaca gcccgacgac gctcgccgga
    12601 aacgccagct cgggtcagaa agcacgaacg gccggtcatt tttcggtcaa cacggcttta
    12661 gcatctgccg aaactgctgc atcagagcga tacgcgactg cgccaacccc tagcccgccg
    12721 aaccgtgaat ccagcttctc cactcttcca gatgatcgcc atcggcgctg cgggcgccat
    12781 tctgggcgca tggatggtct gggcatggct atccccccgg gacagagccc aaccgcttcc
    12841 caacctgtgg gccatttccc cgcggccggt tttcaacgcc aacgagcgcc ggctctaccg
    12901 tcaactcagg gaagcgttcc cccagcatgt ggtcatgccc aagctgccct tggtacgtct
    12961 gtgtcagcca gacgttcagg agcaggtcca gtactggtac gggttgctcg gatccacgca
    13021 cgcgacattc accgtctgca gttcgagcgg caaggcgttg ctggccatcg acctcgaaag
    13081 ccgccgctcg cgttccaagc gaaacctcga aatcaaagaa gccgtgctca aggcatgcca
    13141 gatcaagtac ttcagcatcg cagccaacgg catgcccacc gtcgcggcgc tgcagttgtt
    13201 gcttccgcac accggatccg aagcaccttc ggccgttacg cgagatgacg tcgccagagg
    13261 ccccgtcggc gagtcgatca acgagccatt tgcatccaca aggccctcat ccagccagcc
    13321 actcaaaggc acgggtcgtt ggcaggattc gagcgtgttc atggactcct tcttttctcc
    13381 ggacagccgg atggaccccg ggggcgatgt cgatctcccg catggcgcat cggaatcgac
    13441 ggcgcccagc agcgccgcgg gccattgagc gtgcgcttac gatcaacggg tgcattcgtc
    13501 cccctcaccc gatcccgccc tgcaaactcc tttttccgga ctgaccccgt cggtcgtcct
    13561 tgacgccctg gacagcgtgg gtctggccgg agacggtcgt atcttccagc tcaattccta
    13621 cgagaaccgg gtattccagg tcttcctgag cgatggccga gcggtggtga cgaagttcta
    13681 ccgtcccaac cgctggacgg acgcacagat tctcgaagag cactcgttct cggcagaact
    13741 ggcggccgaa gagatcccga tcgctgcccc ctgggcgctg gaagctgaca ccggttcggc
    13801 gattcagtgc acgctgatcg gcgctccagc cacgctggcg aggatcaaca ctccggacgg
    13861 ggactaccgg ttcagcgtca ccgagcgcaa agccggccgg gcgcctgagc tggaacacgc
    13921 agacacgctg gagtggatcg gtcgattcgt cggccggatg cacgctgtag gcgctcgcaa
    13981 gcctttcgca gtgcggcgca gtctgacgct cgatgagttc ggctggggat gccgcgactg
    14041 gttgctagaa cacggcaaca ttccgccgga gatcgcgccg caatggaaag cggccgtcga
    14101 cgccgcgctg acgttggcca gtcgaggctt cgagcggctg ccagacatcc agaacatcag
    14161 gttgcacggc gactgtcacg caggcaacgt gttgtggacc gacgccgggc cgcacttcgt
    14221 cgatctcgac gattgcctca ccgggccggc cgtgcaggac ctgtggatgc tgctttccgg
    14281 cgaccccact gcagcccgcg tgcagatgag gtcgttgctc accggctacg agcagttcat
    14341 ggacttcgac gaccgacaac tcgcgctgat cgagcccttg cgcaccctgc gcatggtcca
    14401 ccagagcgcc tggctggcca agcgctggtc cgacccggcg tttccgccga gcttcccctg
    14461 gttcgccagt ggcgcctact ggcagcagtc ggcacagcaa ctgctgcagc aggtcgagca
    14521 aggctaggcc aacagcaggc tgttgacccg gcgcacgtag tcggccggat cctcaagctg
    14581 gccaccctcg gccagcacag cctgatcgaa caggatgtgg gccagatcgt cgaagtgctc
    14641 gcccttgtcg agcctgctga ccagcgcatg ctgggcattc acttcgagta tcggcttgga
    14701 ggcaggcacc ccggtctggc cggactgctt cagcagtcga gccaggtgtc cgctcatgtc
    14761 gctctcctcg accaccagac aggccggaga ctcgaccagc cgggtcgtga cccgaacatc
    14821 cttggcgcga tccctgagtg tctccttgag gcgatcgagc acgggtttga acgaggccga
    14881 ggcttcttcg gcctgcttct tctcctgctc gtcctgcagc gagccgaggt cgaccgcgcc
    14941 cttggcgacg ctttgcagcg acttgccgtc gaactcgtgc agatggctga gcatccactc
    15001 atcgacgcga tcggtgaaca acagaacctc gatgcccttc ttgcggtaga tctccaactg
    15061 cggactgccc ttggcggcag ccaggctgtc ggccgtgatg tagtagatcg actcctgacc
    15121 ttccttcatc cggctcacat agtctgcgaa cgacacgcct tcgtcggcct gcgtcgatgc
    15181 gaagcgcagc agcttggcca gccgctcgac gttcacgtgg tcctctccga cgccctcctt
    15241 cagcaacgta ccgaaatcct tccagaacgc ggcgtacctg gcgcgctcgg cctcgtcttc
    15301 gctgttggcc agcgcttcga gcatggtcag cacgcgcttg gtcgaacctt cacggatcgc
    15361 acgcacgtcg cggctttcct gcagcagttc gcgactgacg ttcagcggca gatccgcgct
    15421 gtcgatcacg cccttgacga agcgcaggta gaccggcatc agcgcctcgg cgtcgtccat
    15481 gatgaagacg cgcttcacgt agagcttcac gccgccgcgc ttgtcgcggt tccacaggtc
    15541 gaacggcgcc ttggccggga tgtagagcag ctgcgtgtag tcgctgcgac cctcgacgcg
    15601 gttgtgcgta taggccagcg gcgcttcggt gtcgtagctg atctgcttgt agaactcgtt
    15661 gtactgctcg tcggtgatgt cgctcttgct gcgtgtccac agcgcggcag ccttgttgac
    15721 aggcgcccac tcgtcgcgct tgacctgctt ggcggcttcg gcgtcccact cttccttctg
    15781 catcagtatg ggcagggaca cgtggtctga atacttgccg atgatcgatt tcagcttcca
    15841 gccactgagg aactcttcct cgccgtcgcg cagatgcagg atgaccgagg tgccacgctc
    15901 ggccctgacg atcgactcga tctcgaaatc gcccgtgcct tcggagctcc agcgcacccc
    15961 ctcgtcgggc tgagcgccgg cacgccgtga ctcgacggtg attcgatctg ccacgatgaa
    16021 gccgctgtag aaaccgacac cgaactggcc gatcagctgg gcgtccttct tctggtcgcc
    16081 gtcgagcttg gccatgaact cgcgtgtgcc gctcttggcg atcgttccga ggttcgccac
    16141 cgcctcttcg gcgctgaggc cgatcccgtt gtcgtggatc gtcagcgtct tggcttcagc
    16201 gtcgaagctg acccggactt ccaggttcgg cgcatcttcg tacagctcgg gatggttgag
    16261 cgcctcgaaa cgcagcttgt cgcaggcatc ggaggcgttt gaaaccagct cgcgtagaaa
    16321 gatttccttg ttcgagtaca gcgagtgcgt gacgaggtgc aggatctgct tgacctcggc
    16381 ctggaaggaa agagtttgtt tggtcatggt cgctttggaa agtgatcttt gatggcgaac
    16441 gaaaagcggg gatccgcagc ggactccttc cgcaagacgg ggaatatggg ggccgggccc
    16501 cgaggttcaa gccccggggc cgttcagggt gaatagcagc ccatagccga ctgccgcagc
    16561 cgctgccagg aagggcaccg aacagagatg gaaactgatc caggcacggg agtcctgggc
    16621 catccgcaat gcgatcaggt tggccagcga tccgaccatc cacccgaagc cgccgacgtt
    16681 ggcggcgtac gccatcacgc gccagtcctg cgaatactct gccagcccga tgactgcggg
    16741 gacgttgctg atgagctgag aagcgccgat gccggcccag tagagccgca cgggatccgc
    16801 cagctccagc ccgccgatcg cagactgcgc cgcatcaagt ccggcaacaa gccgcagatc
    16861 aatgaacatc aacgtgaaca ccagcaacaa cccccagtcg atccgggcga gcacgcgcgg
    16921 gcgcacggca gcgatcaccc cggccgccag cgcgcagccc cagagcgctt caccgcggtc
    16981 cgtcagcacc aggaaaggca cgtacatcgc cagggcggcc agcagcaacg ggcgatcgag
    17041 ccgctcgctg ggcgaacggt cttcgatgcg aagcggggtg gctgcgaaga acaaggccac
    17101 cagaacagcc agaagaaccg acagcacggc caccagcggc gccatttgaa tggtgaactc
    17161 ggcaaacgac acctgagaca actgccaaag gaacaggttc tgaggattgc cgatcggcgt
    17221 cagcgccgag ccaacgttca ccgcaagcgc ctcgaagacg atcagacgcg tcgccggaag
    17281 cggggtgatg cgacacagac ccagcgtcag cggcaccacg acgaagagcg cgacgtcgtt
    17341 ggtcagcacc gtagagagag cggcggcggc cgacaccaga ctcagcgccg cgacgcgctc
    17401 tgtcgacatt cgccgcacca gcgcacggcc gagccacgcc agtgcgccgc tcgactcgac
    17461 cgcctgcgtc agcacgagca atcccgccag cgcggcaatc gtcgcccaat cgacgagcat
    17521 cggataagtc ggaatgatct gcggttgcgc ggccgcgagt cccgccagca ccagcaacag
    17581 gacccagaag aatcgatctc gtctcagtgc cgccagcggg gatgaccagg gcgttgaagg
    17641 cgaggcaggc cgagccgggt cagccatgcg ccagcgaggc atcccatgcg tggacggtct
    17701 ggaactgctc gccgagcccg tcgatgccga ggcgcatcgt gtcgcctgca cgcaggaagc
    17761 tcggcggctt gcgccccagg ccgactccgg gtggcgtgcc ggtggtgatc agatcgccgg
    17821 gattcagcgt catgaagcgg ctgatgtagc tgaccagccg ggccacgccg aacaccatcg
    17881 cgccgctgtg gccggtctgc gcccgctgac cgttgacgtc gagccacagc ccgagcgcct
    17941 gtgggttggg cacctcgtcg gttgtgacga gccacggacc gacaggcccg aaggtatcgc
    18001 agctcttgcc cttgtcccag gtgccgccgc gctcaagctg gtactcgcgc tcggagatgt
    18061 cgttgacgat gcagtaaccg gcgacgtggc ccagcgccgc gtgctcgctc acgtaacggg
    18121 cacgggtgcc gatgacgacg ccgagttcga cctcccagtc ggtcttcacc gaaccctgcg
    18181 gtaactgaac cgcgtcgttc gggccggcga ggcagctcgt ggccttcatg aacagcaccg
    18241 gctcggccgg gatctcgagt cccgactcgg cggcgtgatc cgcgtagttc aacccaaccc
    18301 cgacgaactt ggcgcagccg ctgtaaggca cgccgatgcg ccccggctcc accgccggca
    18361 ggctggccgg atcgatatcg cgcagacgct gcaggccgtg cggcgacagc gtcgcgggaa
    18421 cgatgtccga aagcactccg gacaggtctc gcaccacccc ccgggcatcg acgagggcag
    18481 gcttttcgaa ccccatcggg ccgtggcgca tcagcttcat gacggtgtgg gcaagtggac
    18541 ggagcgtcga ttctcgggcc atatacccga gcgcgttccg gggagcccat acgagcccac
    18601 ccatttacca tccattcaga tggtttatgg atgctgatag gatggcgcat gacatccaac
    18661 cgcgtcacct cggcggaagc gccgcccatc gccgcagccg ggcagccagc cagccggcag
    18721 gcaaaggaag gcggcgcccg acccgcccgg acgcccgact cggaaaagat cacgatcaac
    18781 ctcggtcatg tcgatctcgg gcaggtggac ctgctcgtca acgaaggctt ctacggcaac
    18841 cgcaccgact tcatccgcac cgccatccgc aatcagctga cgcagcattc cgacgtgatg
    18901 aaacaggtgg tggcgcggaa gatgttcgtg ttcggcatgc agcacatcag ccgtcacgac
    18961 ctggaacagc ttgaactggc tcgacagaag ctcaccttgc gggtggtcgg tctgacgacc
    19021 atcgctagcg acgtgacgcc ggaactggcg ctggccacga tagacacact ggaggtgctg
    19081 ggcgctctgc atgccagcgc cgaagtcaaa cgggccctgt ctggccggtt gaaatgatcc
    19141 cgtctcagcg cccccgaaat ccccccgcct cctgaccttc cacgaaagcg cccatgtcct
    19201 ccacccttca acgctggatg aaagaagtga cacggctgac ccggtccggt cgtctcgccg
    19261 atgccacccg ggcaattcag gatgcactgg gtcgcaaacc gacgatggga tcgttcaatg
    19321 cgccccctgc cgaagtgaac tcgcaaaccg cggcagcagc atccgctgcg cgactcatcc
    19381 cggagcccgt gccggtcgag acctcgaaag ccatcgacga cggaaccatc cgtacaggcg
    19441 agtacagcga agctggattg accagaagct acctcctgta cctgccgccc acggcatccg
    19501 cagagcactc gggtacaccg atgcccctgg tcgtgatgct gcacggatgc acacaggacc
    19561 cgacggactt cgccgccgga accggcatga actcgctggc acgcgaacag ggtttcgcgg
    19621 tgctctaccc cgcgcaggcg cagagtgcaa acccgtcgcg ctgctggaat tggttcaagc
    19681 acaaccacca gcgtcgcggc agtggcgaag ccgctgtcat cgccggcatg acgcgctccg
    19741 tggtggccga gcaccacctc gacccctgtc gcgtctacgt cgcagggctg tcggccggtg
    19801 gcgccatggc ggccatcctc ggtcgggcct acccggacct ttacgccgcg gtcggcatcc
    19861 attcggggct cgccagtgga gcggcgaacg acctgatctc tgcgctatcg gcgatgaagc
    19921 atggccctgc ctcgccaacc tcgcctccgc ctgtgaccca aagcggtgaa cccgcagcaa
    19981 gggacgacgg gttctcggct tccatcggtc ccgtgccgac gatcgtgttc cacggtgacg
    20041 gcgacgccgt cgtgcaccct ggcaacggga cacaggcgat cgcggtagcg atcgggccga
    20101 ccggaaccgg tgcgccgagc gccgccagtc cggtggtgga ggtgggcgag tcaaccaagg
    20161 gacgacgcta cacacgtacc acgcacccgg gtccggcggg cgccccagcg caggcggagc
    20221 actggttggt gcatggcggc ggccacgcct ggtccggagg cagggagcag ggctcgtaca
    20281 ccgacccaag tggaccggat gcgacgcgcg aaatgtggcg gttcttccgc gcccactcac
    20341 agcccgacgc cccgcggcac gaaggttgag cccacggttc aaacacctgc aagggagcac
    20401 ttacattgag actcgtgtgc cagttgcggc gtgccacccc gattcgttcg gtcgtctggc
    20461 tggtcacccc taaccgagag gattgaacat gctggcgatc attggcggga ccggtctcta
    20521 cgaactgccg gggatggtga tcgaatctca cctcgatgag ccgacgccct tcggcgcgcc
    20581 ttcaggcgtg atcagccaag gccacatcgg agaccaccgc gtgctgttcc tcgcgcgcca
    20641 cggtgcagga caccggctgc tgccgcacga ggtcaactac cgagcaaaca tctatgcgct
    20701 caagcgcgcg ggggccacgc agatcctcgg tttctccgcg gttggcagcc tgtccgagcc
    20761 gatcgagccg gggcatctgg cggtaccgga tcagtacttc gactggaccc ggggcggacg
    20821 ccagaacacc tttttcggga acggtgtagc agcccacgta tcaaccgccc ggccggtgag
    20881 tgcggcattg gtggattggg tcgaggaagg cgtcaagcgc catgcggcac atcacgccgg
    20941 aagcgcacca atcaccttgc accgcaacgt gacctatgcg tgcgtcgaag gaccccggct
    21001 gggcacgcgc gcggagagtc tgttcttgcg cagcgccggt tgccagttgg tcggcatgac
    21061 caatgtgccg gaagtttttc tggcgcgcga ggcccagatg ggctacgcca cgataggcat
    21121 cgtgaccgac tacgactgct ggatggaaga ccccagcaag catgtcacgg tgagttcgat
    21181 cttcgcgctg taccgcgaaa gcatcgctgt cgctcgcgca cttctggaaa ccttgctttc
    21241 gggcgcactg cccacgcccg aagcggacat ccgcaacgcc ctggagcatg cgctgatgac
    21301 ttcgcaagac cagctgacag atgcgcagcg cgaatggctc aagatattgc gtctctgatt
    21361 ccggcggcag aacccaccag gcgcttgcag gctcagaact ggacttcgcg tctgcctgac
    21421 aggctggacg aaagatccgc ggcctgctgt cgatcggccg cgatccactc gctggccttg
    21481 cacatcgaga acatcaaggc ttcatccgca ctctgccagc ggtgcccgca cgccagactc
    21541 tcgtcgcgaa acacctcgcg cgccttgacg ccggtcccga gcatgcggct cacgacgacc
    21601 gcagcgatgt agccctcgcc ccggggtgca tcgagggccc cggcgtagac ccggtattcc
    21661 ccatcgactc gttcgttgaa ttgcatgatc cgctccccgc tcatcagctc aatcctgagc
    21721 ggcgaatgat cgtttcgtcg agacagatcg gaaatccacc tgcggatggc cccctttgga
    21781 ggggagtacg ttgtccgtcc gaagaccgcg gggcgctggt gaagtcagcc tagaagagca
    21841 ggggcagtca ggcctcggtg actcgcctgg caaggtgagc cagcgcctcc tgaacctgat
    21901 cgacgagaac caggcacaga tcgcccggct cgagtcggtc cagcgcctga tcgatggcaa
    21961 tgaactcgcc gcgaatttcg tcgacgcggc ggcaccgctt cgtgcctgcc agcccgtcgc
    22021 gcagcaactt gagcacttct ccgtcggcgc gcccgcgctg acacgcatcc tggaacagca
    22081 acacgtcgtc gaaggcatcg ccgaggatct gcgtctgctc ccggatgtct tcatcgcggc
    22141 ggtcaccggc gccgctgatg accaccgagc gccgctttgc cggcatcgca tcgaccgcgg
    22201 caaccagggc gcgcatggcg tcgggattgt gtccgtagtc ggcgatgaca gttgcgccgc
    22261 gaaagctcaa cacgttgaac cgacccggca cgttgtccga gtccgcggag aagctggcca
    22321 gcccgcgtcg cacggcgtcc catgccagcc cggtgcccca ggctgccgcc atggcggcca
    22381 tcacgttctc gacctggaag cggatcgcgc cgttgcgggt caacgggaca tcacgaaggg
    22441 cgacacgctc gcgccacgcg ccctgcgctg caaccagcca ttcgccttca acgcataccg
    22501 tgcggtgtcc ctgtgcacgg tgcgtgacca tcaccggatg ctgccggtcg gaggcgaaat
    22561 agatgacatc tcccgggcag ttggctgcca tggcgacaac gtttgcatcg gtggcattca
    22621 gcactgcgta gccgtttgtc gcaacgttct gcacgatcac ccgcttgagc accgccagat
    22681 cctcgaccgt ggtgatgtag ttcaaaccga ggtgatcgcc ggatccgatg ttggtgacga
    22741 ctgcaacctg gcagcggtcg aagccgagcc cttctcgcaa cacgccacca cgcgcagtct
    22801 cgaacaccgc ggcatccacg tccggatgca tcaggacatt gcgcgcactc ttcgggccgc
    22861 tgcaatcacc actgtcgatc tgccggcctt cgacgtagac accgtcggta ttggtcatcc
    22921 ccacacgcag gccgctggtc gagaacagat gcgcgatcag ccgcgcggtg gtcgtcttgc
    22981 cattggttcc ggtgaccgcg acgatcggaa ttcgtccgtc gtctccgggc acgtagagat
    23041 tggcgatcat cgcttcgccg accggccgcc ccttgccgta ggacggcgac aagtgcatgc
    23101 gcaagcccgg cgccgcgttg acttcgacca ccccgccgcc ctgctcctcc atcggccgga
    23161 gcacgctttc gcacaccacg tcaacgccgc agatgtgcaa tccgacagtc tgcgccgccg
    23221 tcactgcgcg cgctgccatc tcgggatgca cgctgtcggt cacgtcggtg gcggagccac
    23281 cggtgctcag gttggcgttg ttgcgcagca cgacacgacg gcccttgtcg ggtaccgact
    23341 cgggcgtcaa gccctgttcg ccgagacgcg cgacggcaat gtcatcgaag cggatcttcg
    23401 tcagcgaggt cgagtgtccg tcaccacgct tcgggtccgc attgacgaga tcgaccagtt
    23461 gtcgcacggt gtgcgcgcca tcgcccagaa cctgaggagg atctcggcgg gcagccgcca
    23521 ccatgcggtc tcccacgacc agcaaccgat agtcgctgcc tggcaggaag cgctcgacca
    23581 tcacgtcgcc gatctcagcg gcaacgcgat aggcgatttc caggtgttcg cgattcacga
    23641 tattgacggt gacgcccttg ccctgattgc cgtcctgcgg cttgactacc accggcagac
    23701 caatctcgag cgcgacagac caggcttcgt ccagactcga cacaggccgg ccgtaaggca
    23761 ccggcacacc cgctgcaaga agcagtgtct tggtcaggtc cttgtcctgt gcaatggcct
    23821 cggccactgc gctggtggcg tcaacctctg cagcctggat gcgtcgttga cgcgagcccc
    23881 acccgaactg caccagactg ccccgcgtga gccgccgcca cggtatgccc cgtgcagcag
    23941 cagcgtctac gatggcgccg gtgcttggtc ccaatcggac gtcctcatgg gtctcgcgca
    24001 ggcgagcgat ggccacctcc acgtcgtacg cctgatcgtg cagcgccgca ctgatcagtt
    24061 cctgcgcgag atcgagcgcc tgtcgcccca cctcttcctc gctgtactcg acgaccacct
    24121 ggtagacacc gggatccaca gtgggcgaag tgcgactgaa cgtcaccgga caacccgcct
    24181 gagcctgcaa tgccagggcg gccacctcca ggacatgcgc gagcgatttg ggacgctgct
    24241 ggctggcagc gggcaagacg ccgatggccg gaaagcgcgc tcgcacgcgc gcctcgaaac
    24301 cggcgatgtg atcgagcatg cactcgggcg cggcacacga aacgatcgcc tcgatggcgg
    24361 tgtgtcgggt ccacagattg ggtccacgca aagcacggac gcgagagact ttcatcaacg
    24421 atgctttctg ctggcggaag tgcgtgaaaa gtcaggccga agcgatggcc gcagccccgg
    24481 tgggcactgc cgctgcagcc acggacggag aagccgcagc cgagccgggc gagaccacga
    24541 caccggccgc gatcaccact ggaaaggtct cgatgccggc tgccatcagt tcgggcgata
    24601 tccccatcgc ccaggccgca gcaaccgacg ccagcagcac gtcgacggga tcggcaggag
    24661 gcatcgccgg atgcgcagga gccacgaacc ggcgtgcggc atacaggaca ttcgtgcaac
    24721 gggtttcgac agctcccttg gccagaacgg cccagccgtc gcgcacgaag gcgattcgac
    24781 caccggcggc ctgatgtgcc gatagcgctt cattcgtctc gtcgcgggcg tagaggagca
    24841 cctcgccatc gcacagggac gccatgtcgg caatgcgagg atccgccgcg tgcagcaccg
    24901 cgactccact ggccagcacg acatcgacct gagtccgcag caccttcacc agctggtcgg
    24961 aatcgtgtac gtcgtgagcg gccagggccg cggcatcgtt gtcgttcagc gccaggtcgg
    25021 tgacgactcc cacatggcag cggtcgtatg ccaaaccgtc gtccagcacc gatggagcac
    25081 cgttttcgat caccaccgca tccaccgatc gattcagcag aagtcgatga ccggcttccc
    25141 accgcgcgcc gtcgctgcga tcgatctgcc ggctgccaag gaacaagccg tctctgcagg
    25201 ccagaccaac gcgccgtccg ctcaggtgca gcagccacgc gaccaggcgc gccatggtgt
    25261 gcgatccgat cgaacctgcg atgccgacga tgtcgattcg gccatcgtct tcctgcggaa
    25321 acaggtggtc gacgatggcc tgccccaccg ggcgcggcat gccttcggca ggcttgagat
    25381 gcatcagcag acccgggccc gcattgactt cgacgatcgc accgccctgc aagtgcagcg
    25441 gcttggagat gtcctccgcc accacgtcaa cgccagcgat gtccagcccg accacgcgag
    25501 ccgccagaga aacctggtgc gccacctcgg gatgcacgtc gtcggtgcaa tcgatggaca
    25561 cgttgccgtt gcgctggatc agcacgcggc gcccggagac cggcacggcg cgaggttcaa
    25621 gtccctgacg ctgaaggtcg aggacgaccg cgccgtcggt gccgaggtcg atgcgcccga
    25681 gcggatgatc ttcggtcagg ccacgacgcg gatcgctgtt gatctgggca tcgatcagtt
    25741 cgaccaccga ggatcgccca tcgccggtca cccaggctgc ctcgcccctt gctgccgcga
    25801 ccacgcgacc gccgaccacc agcagacggt gctcgtggcc gcggatgtag cgctcgacga
    25861 tcacctcgct gccttccggc tcggccactg cgtaggcggc ctcgacgtcc tcgcgggtca
    25921 tcaggtcgag cgtgaccccc cggccgtggt tgccgtcggt cggcttgacg accaccggca
    25981 ggccgatgtc ctgcgccgca tcccaggccg cttcggcgct ggacaccacc tgaccttcgg
    26041 gaaccggaac accgcaggac ctgagcagtg tcttggtcag gtccttgtcg ctggcgatgc
    26101 cctcggcgat cgcgctggtc tgctcggtct cggcggtcca gatgcgacgc tggtgggcgc
    26161 cgtagccgag ttgcacgagg ttgccggcat tgaggcggat gtgtgggatg ccgcgttcgg
    26221 tggcagcagc gacgatgctg gcggtactgg gaccgaaata gcagtcatcg atcttctcgc
    26281 gcacctcttc cactgcagcc ggaaggtcga acggctcgcc gttgatcgtc gacatcagca
    26341 gacggtgccc cgccgccaga gctgcgcgcg ccacgtgctc gtcacgggca cgaaacacca
    26401 tgcggtagat gccgcgccgg gaggtgctgc gagtctggcc gaagccggtg ggcatccccg
    26461 ccaggttcag caactcgatc acgacatgct ccagaacgtg acccgcccag gttcccgact
    26521 cgagccgctc gagaaaacca ccccgctcgc cgacaccgca atggtgctcc atcagggccg
    26581 gcagaagtgc gatcaggcgg tcgctgaagc cgggaagcag gtggctggga tgggcttcga
    26641 gctcacccag gtcgagccag gtttcgagcg cggggcggta agtccagatg ttggggccac
    26701 gcaggtaggt gatgcgcagc aacccgatgt cgtcgaatct cttcatgttt tcttcgcgat
    26761 gttctcagga cgggctgctc ggcgagaatc gcgcactctg ttcggtgcca ccgcatcgcg
    26821 gtcatcgacc ggtacagaac tcatatgcag catctccatc cggttcaaga cccctctcat
    26881 cggcctgcgt cagtcgcgaa gtcgcagcct gttcctctcg agtccgacga gaacgcgctc
    26941 ggatcgctgc tggttgactt gtccggaaat ctgcgtttcg tcagtggcga actgatcctg
    27001 accaaccggc gcctgctcgc cagggtcgac gacggcaacg aagcgccggg gagcgagacc
    27061 gcgtgggtca gctggcgact ggacgagccg gcagccgtcc ggcttcgcct gctccatacc
    27121 gaccacggcg gcatcggagc actggaactg cacgacgacc tcggcaggct ggcaatctgg
    27181 cgattcacgc tggcgcaaaa catccttgca ctgcgcctgg tcaccctgtt cgatgcgcat
    27241 caggcccggc gtccctggcg tgcaggtgct gacgaactcg gcacgacggt cgacgaaccg
    27301 gccctgtgcc cttcgtgcag ggcccccctg ccgccagaca gcgacgactg cccggtttgc
    27361 agccgtgaag ttcaggctgt gccgtcgacc tgggtgctgc tgcgcctgtg gcgcttcgcc
    27421 aagccctacc gaaagccctt ggccggtggt ttcgcactga ccttggcctc gactgccgcc
    27481 accctggtgc cgccctatct ggcgattccg ctgatggacg acatcctgat cccgttccag
    27541 aacggcaagc agatcgaccc gatgcacgtc ctgatctacc tcggcggcct gttgctggcc
    27601 gcgctgctgg cctgggggct gggctgggcg cgcacctacc tgctggccct ggtcagcgaa
    27661 cgcatcggcg ccgacctgcg caccgcgacc ttcgagcacc tgctgcaact ctcgctcgat
    27721 tacttcggcg gcaagcgcac tggcgacctg atggcacgca tcggcagcga aaccgaccgc
    27781 atctgcgtct ttctttcact gcacgcgctg gatttcgcaa ccgacgtatt gatgatcgtg
    27841 atgacggcgg tgatcctgtt ctcgatcaac ccttggctgg cggtggtcac gctgctgcct
    27901 ctgcccttca tcggctggat gatccacacc gtgcgggatc ggctgcgcac aggattcgag
    27961 aagatcgatc gggtgtggtc cgaagtcacc aacgtgctgg ccgacaccat ccccggcatc
    28021 cgagtcgtca aggcgtttgc ccaggaaggc cgcgaggccg agcggttcag agtcgccaac
    28081 cagagcaacc tcctggtcaa cgacaggctc aacaagacgt ggtctctgtt cacgcccacg
    28141 gtgtcactgc tcaccgagat cggtctgctg gtcgtctggg ccttcggcat atggcaggtt
    28201 tccaggaacg agatcactgt cggcgtgctc accgccttca tcgcctacat cggccgcttt
    28261 tacggccggc tcgattcgat gagccgcatc gtctccgtga cgcagaaggc cgctgccggc
    28321 gcgaagcgga tcttcgacat cctcgaccac gtgtccaacg tgccggaacc ggcgacgccc
    28381 ttgccactga ccaacccgca gggccgcatc gagctgaacg gcatcggctt tcgttatggc
    28441 aaccgctcgg tgatccgcgg gctcgacctc gagattcgcc ccggcgagat gatcggtctg
    28501 gtcggacaca gcgggtcggg caagagcacg ctggtgaacc tgatctgccg cttctacgac
    28561 gtgaccgatg gcgcgatcaa ggtcgacggc atcgacatcc gccgctacgg cgtcgccgac
    28621 tatcggcgcc acatcgggct cgtcctgcag gagcccttcc tgtttttcgg cacgatcgcg
    28681 gagaacatcg cctatggccg ccccgacgcg agccggcagg acatcgttgc cgccgcacgc
    28741 gcagcgcatg cacatgaatt catcctgcgg ctgccgcagg gttacgactc gctggttggc
    28801 gagcgcggcc aggggctttc gggcggcgaa cgccagcgca tcagcatcgc gcgcgcactg
    28861 ctgatcgatc cgcgcatcct gatcctcgac gaggccacgt catcggtgga caccgagacc
    28921 gagaaggaaa tacagaacgc gctcgacaac ctggtgcagg gtcgcacgac gatcgcgata
    28981 gcgcaccgac tgtcgacgct gcgcaaggcc gaccgtctgg tcgtgatgga ccgtggacag
    29041 atcgccgaag tgggcccgca cgacgatctg atcgcgcggc ggggggccta ctggaggctg
    29101 cacgaagcgc agcttcgcca ggcggcggcc gacgattggt cgccggtttc cgacggcgtc
    29161 gacgcgccgg tcgctgcggt ggcggccggc ccatcgagtc actccgagtt gccagcatga
    29221 gcggcgaacc cttccaactg gaacgcaacg cccatggtcg cctggtgctg atcacgtccg
    29281 gcggcgagag ccatgcgggc gtggtgccga tacgtgcgtt tccgatagcc gcgccacaag
    29341 aaggcctgtc gctggtcgat gccgacggtc gggaggtggc ctgggtggac aggctcgatc
    29401 agctggccga gccgttgagg gcgctgatag aggaagaact ggcaagccgc gagttcaccc
    29461 ccgagattct ccgcatccgg tcggtgtcga cctactccac gcccagcatc tgggaagtcg
    29521 ataccgaccg agggcctaca cgtttcgtgc tgaagggaga agaagacatc cgccgtctga
    29581 aggacggcgc gctgtggatc gcggacagtc acggcatcca gttcgtactg cataaccgaa
    29641 aggcactgga ccggcggtca cgccggctgc tcgaacgctt tctctgagca gccggcggct
    29701 atgagccccc gcctatccag ccgccttcgt caggcaggtc ctgcgcaaaa gccaggccgc
    29761 ggtccagcaa agcatcgaac gaaagcgcat cgaccgctgg cgagcacagc gaaccctgat
    29821 actgcctgat cccgatgttg cgcaggaaca ggcgctgcgc ctcggtctcg acgccctccg
    29881 ccaccatctc gaggtggagc gcattgccga ggttgatgat ggccttcacg atgcccacat
    29941 cgacctcgtc tgccggcaat ccctggatga agctgcggtc gatcttcagg cgcccgatcg
    30001 ggaagcgctt gagataggcg aggctcgagt agccggtgcc gaagtcgtcg atcgcgagct
    30061 tgacgcccag ccgggccagc gcctgcagcc tgtgcagcgc ctcctgcgca tcctggatca
    30121 ggatggactc ggtcatctcc agctccagcc actgcgggtc gagcccgacc tccgccagcg
    30181 cctgcgcgac gctgtcgacg aagtcgggct gctggaactg cagggccgac acgttgatgg
    30241 acatcagcaa cctgtgcccg cgaccatgcc acagcgcggc ctgctgcaca gcctgtcgca
    30301 acacccaggc accgatggag acgatgaagc cgctgcgctc ggcaaccggg atgaattccg
    30361 tcggcggcac gtcgccgagt tcgggatcgc gccagcggat cagcgcttcg gctccgacga
    30421 tctcgccgct gtcgaggtca gcctgcggtt gatagtgcag acggaagcgg cctgaagcca
    30481 gcgcgtggcg catggcatgg tcgagtttca cccgggtgcg cagtgcgacc accgtggccc
    30541 ccgcccgctg atgatggaat cggtaacctg caccccctgc ggatttcagc tcggtcattg
    30601 ccgcgtcggc ggcctgcagc aaatcgtcgg gcgtggcgcc atcgccgggg aagagcgcga
    30661 tgccgatgct ggcagtcagc gtcaggttca ccccgtcgat cacgaacggg gcctgcaagg
    30721 cctcggtgag acgacgcgcc gcaccctcgg cgcgctgcat gtcggcctga tgcaccagca
    30781 tcacgaattc atcggcaccg aggcgcgcca ccgcatccac ctgtcgcatg gtgtcggtca
    30841 gtcgcctggc gacctcgacc agcacgcgat cgccggcggc cgagccgagg gcatcgttga
    30901 ccggcttgaa gcggtcgaga ttgaggtgaa gcaaggcaaa cgccacgccc tcgcgctgcg
    30961 ccagcgccag cgcgtactcg atgcggctgg cgaccacccg gcgattcggc aacccggtca
    31021 acgcatcggt gctggacatc gcatcgattc gccggttcgc ctcgaggctt tcgctgatgt
    31081 cccgaaacga gaacacccgg ccgatcggct gcccgcgatt gcgctgcggc agcgtgcgtc
    31141 gctccagaac cttgccgcaa cgcaaggtca gcacgtcact gccttccagg gtcatcgact
    31201 cgtcaaggga ctgcaggcgc ctggtgtagc ccaccggatc agacacccga cgcagcatcc
    31261 aagcggaaac ggcggcatcg tcgtgctccg ccagcagcgg ctccggaacg ccccagagcg
    31321 ccacgaagcg ggggttgaag ttgacgatgc ggccggtcag atcggtcacc aggatgccat
    31381 cggtcgttga ctcgagagtg gctcgcagtt gcgagagacg ctcttcgagt tcggcctgcg
    31441 tccgcaactc cagactgcga tcgcgcatga cgaccagata ggtctcgttg gtcccgccgg
    31501 gcgcggccgc gtgccccgag ccgaacggta tgcgggtgac acgtcgcagc accggaacca
    31561 gcttgccatc ggcccgactg acaaaggtct gtgaatcggt gctgtccgcc tgatcggcaa
    31621 cgccggattc ccagaaaacc acgtcttccg gtgtggctgc gagctcgagc gcatcggctc
    31681 gacacagtgc ctggagatcc atcccgagca aggtcgcggc ggcggcattc gccgacagga
    31741 tgcgcaggct gtccggatcg acgagccatg ctgcgtcgag caggccttcg atcaacggcg
    31801 acaagccccg taccgcatcg tcgccagtcg ccgacaagga ttgcgacgcg gggacagccc
    31861 gtggacgaac tgacgccggc gcgccgacgc cgtgcagtgg gtacggcgtg cgctgaggca
    31921 tcacgacacc agttccaggt ccccgacgct ctcgaagaaa tagctgatgc gcggacgagg
    31981 cgaaaggtac gtcagggtct gctccggcag agcggtgggc ttcaggcttc gccggatgcc
    32041 cagaccgccg gcggtctcga ggtcgaggat caacgcctcc tcgcgcggca cccccgtttc
    32101 gtgtaccaga acccggggct tcagcggacg cgacgaattc acccccacca cgagcgcgta
    32161 cctgtcatcg gtcagttgca ccaccgagcc cgccggatac acgcccatca tcttgatgaa
    32221 ggcacccagg atggcagggt cgtacttgtt cttgccttgc gcgaacatca gcgccacggc
    32281 ctcgtgcggt gtgacggcct tgccggggtt ggccgggttg cagaggttgt cgtagcggtt
    32341 gaccagcgca acgatgcgcg cgggcacagt ggtgcgatcg ctggtgagct tgagcgggaa
    32401 gccgctgccg tcgatgagtt cgtggtgctg tgcgatcacc gtggaggcgc cgacaccgag
    32461 ttccatgcgt cgtgcctgcg cgatgccatg cacgacatga gtctcgtaga gcttctgctc
    32521 ggcagtggtg tgatgttcgt cccgatgacg gaagcgctcg gggatatctc ccttgccgat
    32581 atcgtgcagc aacgccccga ggcccaggtc gagcatctcg gtctcggaaa gtccgaaagt
    32641 gcgccccatg agcagcgaga tgatgctgac gttgatggca tgggcactgg ccttgtcgcc
    32701 ggcagcttcg ctgagcagcc ggatgcaaag ctcctcggag ccgatcatct tgtcgaccag
    32761 ctcacgggtc agcgcctggg ccccctggcg cgcttgcgca ggttggctga ccaccgtgtc
    32821 gaacaccacc ttggtcgctc gcgcggcctc gttgaaccgt cgttcgcacg cggccagagc
    32881 ctgacgctgt gcgctcaggg cagcacggcg ggccgcgcga gcctcgagcg ctgccgcttc
    32941 cgcaggatcc acttcaggag cagtgacttc ctgcgccgct cccgcactcg cgggaaggac
    33001 ctcgggatcg ctgagctcgg ggctccagcg caacacgctg atccccagtg aacgcagggt
    33061 gacgagctga tcctcgctgg tgatcttgaa gctcgatctc gggaacggat gcgacagcca
    33121 tccaccttcg agatgaatga acatgcccag ccgcaaggcg gagacgtcga tgggttccga
    33181 ggattgggtg atttgcatga ctgccgccaa cccctagatt accagccaca cacacgtccg
    33241 gggggtgctg acaccaccgc agatcggtca aacagcctcg gaaagctggc ggaattcgtc
    33301 tttcaacttg ccgatcaccg ttgcgacatc gatcggcttc gtccagtagt cgtcgaaacc
    33361 catcgatagc gcatgctgga tctggtgcgg caacgcatcg gcactgagtg cgatgcatcg
    33421 cagatgacgg gtctgctcat cggccttcag gcgctccatg acctcgaaac cggacagccc
    33481 gggaaggttc agatcgagca acaccagcga caactcgttc tcgagagcaa tctccaatcc
    33541 ctccggtccg gtggccgcgg tcagcaggcg ccagttcggc tgcgaacgga agatctgctc
    33601 catcaggatg gtgttgacct cgtcgtcctc tacgtagaga acgacctgct cgacaccgtt
    33661 gtcccaggtc gacggaaccg ctacggggga acggacgtcg ggtgccttcg gccgacccgt
    33721 cttcgcaagc ggcaatgaca gcacgaaacg cgagccacgg cccggccgcg agagcacatc
    33781 gatcttgccg cccatcgccc gcacgagctt gcgcgtgatg accagcccca gcccactgcc
    33841 ttgcgtttcg gtcttttcgg cgccgagccg gttgaagggc tcgaacagct tgtcgagttg
    33901 ctcctcggtc atgccgatgc cggtgtcatc gacggtcaac acgccttgct cacccgcttc
    33961 gaagcgaacc cgcacccgac ccttggggcg gttgtacttg atggcattgg tcaacaggtt
    34021 ggtcacgacc tgctccagtg catccggatc ggcgcgcact ggaccggcgg tcaatggaat
    34081 ctggaccagc agttcgatgc cgcgctgctg agccagcggc atcacttcga cggcgcaccg
    34141 ctcgacgagg agcctgacgt cgatcggccg attgcgcaga cgaagcctgc cttgctcgat
    34201 ccgggagatc tcgagcagtt gatcgatcag cgacaggagc cgcgatccgg attggcgcaa
    34261 gacctccagg cgctgcgcct gcgccggtgc cagcggatgc tcggcatcca gcgccatcaa
    34321 ctgggcgaac cccaggatgc cgttcagggg cgtacgcagt tcgtggctga cgcgcgacat
    34381 gaactcgctc ttgctgcggt tcgccttttc cgcgtcgagc ttgtcgcgct tgagcgcatc
    34441 aagcgtcttg cgctgggtta catcggtgca cagacccact cgccgctggt catcggccac
    34501 ggcttgaagc acgaactcca gcgtccgcgg gccctgtcca tccgccagat cgaaagagag
    34561 cagaagtcgg gtcacctcgt ctggtgcgct cggcttggca agcaaggccc gcgcggccgt
    34621 gcgatccgcc tctgcgacgc gctcgagcca cgagtcgaac gcccatcggg actcggccgg
    34681 atcgatcccc aggtggcgac aggccacctc atcgaactgc agggatcctg cgagcagact
    34741 gatctccagc atgccgatgt ctgcagctgc aatggcacgc tgccagcggt tgtgctggcg
    34801 ctgcaattcg agatcgactg ccggcgggag ggccgcctcg ttcaagagaa cgttcaggta
    34861 caggccgcca tcgaccgaac ggtccaccat ggcgctggca tcgagcttcg cccactgtcc
    34921 attcggacgc cggattcgca gggtctcctt gatcggcaac ggcggctcgt tttcctgtgc
    34981 gcccaggcac tgaaggcgcc gcaggtcctc gggatgcacg cacgctgcca gtggacgggc
    35041 atcgttgcgc agggcgggca agccgattcc gcacaagccg atgaacgcgg tcgatgcgta
    35101 agggaccgac caccggccat ccgccgagcg gcacagacga accaccatgc ccggcagctt
    35161 gtcgagcagg gactgcatct ccaggatcgg gtcgcgatcg tcgcgccggc gcaggccccg
    35221 gacggccgac tctcgcagat cggttgtcga actgggcggc tcggtgctgc gtgaaagtcg
    35281 ggccgtcgga tgcatcgttg ttgggtgaga catgcgatcc actggtcgcg ctgctacaaa
    35341 ggattcgaag gctttcgccg gaggcgtata tgtaacattt aacaacagaa agcatcgcac
    35401 cgggaaaccc gtttcgagcc ccgcattcag gggttgaact gcgccgaact cgcgttcacc
    35461 ccccgagttg agtgggccct cttcgccagg catccggagg gttcaatagg ccagtccatg
    35521 ctttttgccg gcgctcgcgg catcatcatc acctcgacac tccccggagt cgccatgaac
    35581 gctgccctca tccaacgccg tgcgctgatc accctgagcc tgggcctggc cacaggcgtc
    35641 gcgtggcccg cggaggtgcc ggaagcacag gccgcaaagg cccgtgaagt ggttcaggcg
    35701 cagctggacg cgttcgctgc cgacgatgct cgccgtgcat tcctgctggc ctcgccaagc
    35761 gcccgcaggc atctgggatc gcccgacaac ttcatcaacc tggtgcgcag gaactatgcg
    35821 gtcgtgtaca ggcccgcttc ggtggcattc ctgaagcccc aattgatcga cggcgtggtg
    35881 atcctcggtg tgcagatgac cgacgcccag ggatcggcct ggctcgccac ctaccagctt
    35941 gagcgtcaac ccgatgggat attcctgatc gatggctgcg aactggtcgc caacgaaggt
    36001 agatacacct gagaccaggg accttgaaag cgaccgtgac aaggctgccg gtcgtctgcc
    36061 cgcaatcctg cgaaccctga accatggcgc agccacgcga actcagcgcc gtcgagcttg
    36121 tgcgggccta ccgggagcga aggctgtcgc cgatggaagt cgcccacgac gttctgcggc
    36181 acatcgatgc ctgggagcct tcgctgcacg cgacctacgc actggatgcc gatgcgacgc
    36241 ttgcacaggc ccgggccgca gaagcgcgct ggctgcaagg aactccgctg agcgcactcg
    36301 acggcgtccc gtcgctgatc aaggaaaacg tggccacccg aggcgtgccg gtgccgctgg
    36361 gaacggcggc caccgaattg ctgccggcca ccgacgatgc gcctccctcg gcacggctgc
    36421 gcgaggcggg cgcgctgatc atgggcaaga cgacgatgcc cgacctggga atgctgtcgt
    36481 ccggcctgtc cagcctgcac ccgctgacac gcaacccctg ggatcttcgc ttgaaccccg
    36541 gcggcagcag cgccggagcg gcagcgggag ccgccgccgg ctacggtccg ctgcacgtgg
    36601 gcaccgacat tggcggttcg atccgactgc cggcaggctg gtgcggcgtg ttcggcctga
    36661 aacccagcca cggacgcgtg ccgatcgggg caccgtaccc tgcgcgggtg gccggcccga
    36721 tcacccgcag cgtgaccgac tcagcgctga tgatgtccgt gctgagccag ccggactggc
    36781 gcgatcacat gagcctgcct cccgagcgta tggattggct tcaactcgac cgtgatctgc
    36841 gaggcctgcg catcggactg atgctcgatg ccggttgggg cctcgcgacc gatcccgagg
    36901 tcagagccgc agtcgttgcg gccgcacagg cattcgaatc agcgggtgcg atcgtcgagc
    36961 cgctgaagcc gttcgccacg cgcgagatgg cagacggcat cgaccgcttc tggcgcatcc
    37021 gcgcctgggt cgacctgcag gcgttcagcg ccgaacgcca gcagcgcgtg ctgccgttca
    37081 tattcgagtg ggctcggggt ggcgccggac tgagcggcac cgatgtcatg aagggctaca
    37141 cccagacgct ggcgatgcgc gcagccaccg tggccgcctg ccaacccttc gattacgtgc
    37201 tgtcgccagt ggcgccgatg accgcgtttc ctgccgaact cccgtgcccg acgaatgacg
    37261 ccgcgcggcc tttcgagcac atcgcctaca cccttgcctt cagcatgagc gagcagccag
    37321 ccgccagcat caacgccggc tactccgaat cgggcatgcc gatcgggctg cagatcgtcg
    37381 gtcgccgctt cgacgacctc ggcgtgctgc agctgtcccg cgcctgggaa cagatgcgag
    37441 ctgcgcaacg cccgtggccg gcagcaccgg gctgaatcca gagcagcgca ggtcgcaccg
    37501 ccaggtgcag gcagggtggc atttgtcgca ctggcgccgc ggactcgccg cgttgccgtg
    37561 cagccaatca cgctcgcctt ctattccttc gcagtgtcct tgcgcacaac tgcgtgtgga
    37621 tgcagtctga ccgtgaacca cctgggggtt attgattgga agacctgagc accaccctga
    37681 accgcctgac catggagcag cacttgctgg ccggagtctt cctgctcggc tacggtttgg
    37741 cgctcggatc gatgttcagt ccgaaagggc ggctgcgggc ggcgttgctg gccctgctcg
    37801 cagccatcgc tttttctgca cggacccatc cttgggaaca cggtgtgctc ctggtgggct
    37861 tcgcgatcgg aggtctcgcg gttttcatcg cgctggcatc tgctttcagc cgactcgggt
    37921 tgcgccatga agccaccgtc gggcccatgt cagcgtccag cagcgcggcc ggccgactgt
    37981 cggacaagct gcgcggcacg gcacgacaca ttgctccggc cgcaggactg gctgcgcgca
    38041 ggcgacgccg cagggctcgc tccgtctgac cttgcaggtc atcgacatcc ccgcgagtga
    38101 cgggacgccg agttacacac ggttgcgcaa gaaccctacc gatttccctg ccacgcgccg
    38161 atatttccgg atgcggctga ggccgacgcc attggcggtc tccgacaccg tgcccggcac
    38221 caaagaccgc agctgcgagg acacaaatga tttggaccaa gacagccacc ggacgagccg
    38281 agatcggtgc ccgcgctctg gtgcatgagc gtgccaggcg caatctgctg ctcctgatcg
    38341 atggtgtgcg cccccaagcc gcactgctgg acagcgtctc cggcatcggc ccggacgatt
    38401 tcgaacagct ggcccgcctc ggcctgatcg ccagggcgcc ggcgggctcg acgccgcccc
    38461 cgcggccggc ggtcactgtg gcgccggtgt cccggccgac ttccctgccg gatcgcgacc
    38521 ccggcacggg cgaacacctg cccacccaga actacgggca actgacaacg aagctcaccc
    38581 agctgatatc gagcgaactg ggattgcgtg gctttctcct gacactcgcg gtggaaaagg
    38641 cggctgacat caaggatctc gcctcggtta ccgaacgcgt tctggccgag attgccaagc
    38701 gcaaggggga tcgggtcgcc gagacggcgc ggcgaagtct tttcggcgcc tgaagcgcct
    38761 tcgcaaagac atcagtcagc gccccaagag cgtgcgaagg ggggagaatc ggggttgagg
    38821 aactttgtgc ttgtgcactg aaccacggcc cccaattcga actcgaacga taaatgaccc
    38881 cgtcctgaga cgccggtttg catcgacaga catgaccacc gccctgctga gatttctcgt
    38941 ttcgatcctg cttgctcttc aggcaggcct gatcacgcct gccgcagcgg ctcaggactt
    39001 cctgccgcct gagcaggcat ttcgcttcga ggcgcgcgcc gtcgatgcgc gcacggtcga
    39061 gatcgttttc agcgtggctg acggctacta cctttaccga gatcgattcg ctttcgcagc
    39121 ggatggcctc gacgtgcgac tgggcgatgc gcacattcct gccggcaagg tcaagttcga
    39181 cgagaccttc ggcaaggacg tcgagacaca tcgaggccgg gtcgtcatcc gtgtacccgt
    39241 cgaatccacc ccggaatccg tcgcgctgac cgtcaccagt cagggttgcg cagacgctgg
    39301 cctgtgctac ccgccgatgc agagtaacgt gcggatgcaa ctcgcgggac tgggggccgg
    39361 cctgagcgca ggcctcggcg acgagatccg ggctgcattc gacggcacac caggcctcga
    39421 cagcgccctg cgcagcgggc atctgtggat cgtggctggc gtgttcttcg tcgctggcgt
    39481 gttgctgtcg ctgacgccgt gcgtgctgcc gatgctgccg atcctgtcgt cgatcatcgt
    39541 cggccagtcg ggctcttccc gccccaccac acgatcccgc ggtctcggcc tggccatgac
    39601 ctactcgctg ggaatggcat tggtctacac cgcgctcggt gtggcggccg gactggcagg
    39661 cgaaggcctc gccgccagct tgcagaaccc gtgggttctt ggagcattcg gcgcggtact
    39721 ggtcgggctg gcgctgtcga tgttcggggt gtacgagttg cagctgccag ccgccctgcg
    39781 cgatcgtctg gatgccaggg cgagcggcct gaagggcggt cgctgggccg gcgtattcgc
    39841 gatgggcggg ctgtcggcgc tgatcgtcag tccgtgcgtg gccgcaccac tggcgggcgc
    39901 attgctcttc atcagccaga gccgcgacgt gatgctgggc ggcggcgcgc tgttctccct
    39961 ggcggtcggg atgagcgtgc ccctcttgct ggtcggtgca tcggcaggtg cgctgctgcc
    40021 gcgcgccgga gcctggatgg attggatcaa gcgcttcttc ggcgtcctgc tgctgggcgt
    40081 cgcgtactgg atagtcagcc cggtgctgcc cacgttcggc gccggcactt ctgcaacggc
    40141 acccgcggac gccgagattc atttcaagcg gattcgcaac gtgggcgaac tcgaagcagc
    40201 gttgcgcagt gcggcgcgac cggtgatgct cgacttctat gccgactggt gtgtcagctg
    40261 caaggaaatg gagcgctata ccttcgttga tccgcgcgtg cgcgccaagc tggatcaagt
    40321 gctgctgttg caggtcgacg tcacgcagaa cacgtccgag gacaaggaat tgctgaagcg
    40381 cttcaacctc ttcggcccgc ctggaatcgt gttcttcgat gcgcagggaa ccgaagtccc
    40441 gaaccggcgc gtcatcggct atcagaacgc cgaccgtttc ctcgaaagcc tcgcccaggc
    40501 cgggctgtga tcgtcaggtc tcggcctgag cctgttcgag ccggcgtgcc agttcatgca
    40561 attgcgagcg cagcagcagg aactcctctt gacgcaggcc ggtctggcac gcgacgcgct
    40621 gttgcaccag agcagccgga gcatgcaggg cccgcccggc agccgtgagc tggaccctca
    40681 ggttgcgctc gttggccgga tcgcgttcgc ggtgcaccag ccccgcagca gccagacgct
    40741 tgaccagcgg cgcatcgtcc gccggcttgg cgccgcgcgg ccagtggttg atgggcgtgc
    40801 cggtgacaaa gcgcagaccg cggtacaaaa atacgccggc aatgagcagc
//
LOCUS       DAOORV010000014        39882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1137_length_39882_cov_7.787857, whole genome shotgun sequence.
ACCESSION   DAOORV010000014 DAOORV010000000
VERSION     DAOORV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 39882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_39882_cov_7.787857"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKV56_03540"
     CDS             <1..186
                     /locus_tag="PKV56_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX66915.1"
                     /translation="DWKKGSPRDNRILFIGRDMPQEVIVQGLKDCLVCAGATAAPPRR
                     AAIKQGSTVRPPFRRVA"
     gene            183..1193
                     /locus_tag="PKV56_03545"
     CDS             183..1193
                     /locus_tag="PKV56_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX66916.1"
                     /translation="MSLIPVTILTGFLGSGKTTLLNRILRERHGQRIAVIENEFGEAG
                     IDNELLVQDTGEQIIEMNNGCICCTVRGDLVRILGELHAKRTAGTLHFDSVIIETTGL
                     ADPAPVAQTFFVDEDIGDHYRLDAVVTMVDAKHAMAQFDAHHEAQEQVGFADRILMTK
                     TDLVSVEELRALEQRVRRMNPRAPIAAAHFGVTPIEQLLDVRGFNLNDVLQIEPDFLT
                     NSDHEHDDEVTSFVFSAEQAFIAERLEDFLTAILQLYGANLMRYKGVIDVEHHDRRVV
                     LQGVHMLMGMDSGSAWARDEHRNSRIVFIGKSMPKDLILQGLEQCLVSAKTAAAKVQE
                     IT"
     gene            1252..1812
                     /locus_tag="PKV56_03550"
     CDS             1252..1812
                     /locus_tag="PKV56_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOX66917.1"
                     /translation="MIRKNPRGDLPQAHESAFVDPTALLCGRVVVHENVFIGPYAVIR
                     ADEVDDEGHMEPIVIGAHSNIQDGVVIHSKSGAAVTIGERTSIAHRAIIHGPCRVGNG
                     VFIGFNSVLFNCRIGEGCVVRHNAVVDGCDLPPGFYVPSTERIGPHTDLNAVAKVNVQ
                     ASEFSEDVARANNDLVAGYKRIQNEL"
     gene            1831..2379
                     /locus_tag="PKV56_03555"
     CDS             1831..2379
                     /locus_tag="PKV56_03555"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HOX66918.1"
                     /translation="MKETIQLSLLRCVLTAGALAMAIQANATEEAHVHGLMHINVAID
                     KQVLTIQLESPLDSLLGFENKPKTTTQRLAVDAMSKQMNDGAALFKPSIAAQCKLTKA
                     TVESAVFQATAPAGDKEEPHADLDASYEFTCAQPDKLTSVEIGLFQAFPRLQKIEVQV
                     AGPKKQSKTSLSRPANTVKLKS"
     gene            complement(2494..4383)
                     /locus_tag="PKV56_03560"
     CDS             complement(2494..4383)
                     /locus_tag="PKV56_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOX66919.1"
                     /translation="MSESVIHFHHGGPLVALVGNPNCGKTALFNLLTGSRQKVANYAG
                     VTVERKEGRLITPAGKTLRVLDLPGAYSLHPRSPDERVTCDVLYGRAKGEKRPDLVVC
                     VVDATNLRRNLRIVMAVKRLGLPCIVALNMTDLAARRGINVDAQALSAELGLPVVATV
                     GIRNEGAGRLRAMLDDPLVWQRVSSPAAHGATESAIVTQADHEVVHSMLERLGLAAVL
                     PHTASDRIDRVVLHPVFGPILLAVVLFLVFQSVFAWAQVPMDAIKSATEWMGAAVTSA
                     MPDGWMRSLVVDGVIAGAGGVVVFLPQILILFFFILLLEESGYLPRAAFLLDRLMGSV
                     GLSGRSFIPLLSSFACAIPGIMAARTIANPRDRLVTIMIAPLMTCSARLPVYALLIGA
                     FVPARSVWGGFQLQGLVLFGLYVAGIAGALAVAWVLKRFTARGQVRSLMMELPAYHWP
                     TLRNLAIGLWQRMEIFMKRVGGIILGLTIALWFLASFPAPPAGATGAPIEYSVAGMLG
                     HALAVVFEPIGFNWQISIALVPGLAAREVAVGALGTVYALSATGEDTAQALVPLISQT
                     WSLATALSLLAWYVFAPQCLSTLATVKRETGGWTMPLLMAGYLFALAYAASYITYRVA
                     LSLGG"
     gene            complement(4380..4598)
                     /locus_tag="PKV56_03565"
     CDS             complement(4380..4598)
                     /locus_tag="PKV56_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOX66920.1"
                     /translation="MRQRFLVRAVQAPPRAPEWAQWLEEIGFMPGEQVELLARGAFGG
                     DPLVVRVGLSTFALRRAEAACVVVEASA"
     gene            4859..5356
                     /locus_tag="PKV56_03570"
     CDS             4859..5356
                     /locus_tag="PKV56_03570"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOX66921.1"
                     /translation="MGPPSIILIAAVARGGAIGRDNQLLVHLPGDLPRFKRLTMGHPI
                     VMGRKTWDSIGRPLPGRRNIVITRQPDWCAAGAERACGFEAALELAGDVEQVCVIGGA
                     EIYALALPRADELLLTEIDADYVADAYFPDWERGDFDRVESESHATEQGLVYSYVRYR
                     RKSHR"
     gene            5460..5729
                     /locus_tag="PKV56_03575"
     CDS             5460..5729
                     /locus_tag="PKV56_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOX66922.1"
                     /translation="MPSFIRPALLSLAMLLSGGTMASPDKCNAGPRDQWQPIETLEKR
                     LRSEGWTIRKSTIHSGCYKIRGKSGKGMRMKAYFNPKTLEILKDE"
     gene            5772..6425
                     /locus_tag="PKV56_03580"
     CDS             5772..6425
                     /locus_tag="PKV56_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX66923.1"
                     /translation="MRLLLLEDDAILGEGLRDFLQTDGHRVDWCKRLCEAKALTGEPY
                     DALLVDWQLPDGSGLDWVRSIRRQGISTPVVIMTARDLLSDRVRGLDGGADDYLVKPF
                     EPEELTARIRAVRRRVSGSASALLAFGDVRLDLTGKVAFLHDVRADLTAREWAVVEAL
                     ALRAGRLVSRSELEALVLGLDNESASNTLEVHISSIRRKLGRGVIETSRGLGYRLAT"
     gene            6422..7726
                     /locus_tag="PKV56_03585"
     CDS             6422..7726
                     /locus_tag="PKV56_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX66924.1"
                     /translation="MTPNTSALPSIRQRLSRALIGVSIGWGIAVSAVVWFAVRHEVDD
                     LLDNALQESAEILFALLSFNSAQLSLNGGGGWLPAPVHDEELVWQIVGADQQVLLRSH
                     RAPAAPLANRRTTALSSVGDEWRVYSLPFDTTGRMLFVAQTGRSRHQARLDAAMFTAS
                     AALLVGVLCAYLLRWRVRRELVPITDLSQAVMQFDPMRPESRLDPARRAELMPMHTAI
                     SELGTRLARRLGNERAFSAHAAHALRTPLAGMVAQLAVAQRVSSAEAQPHLQRTREAA
                     SRLQRVVTALLTLFRTGGEVRRQRVEIADLIGQLSFEQSVISSGGSTHLQVDPDLLAA
                     ALMNLIDNSLRHGASTVSVQSREEPDGRIVIAVHDDGSGIPQADRTRLQSALDAQQYD
                     GGMGLGLMLADLVARAHGGQLRMKSSASGCTVELDLGIPAAD"
     gene            complement(7749..8381)
                     /locus_tag="PKV56_03590"
     CDS             complement(7749..8381)
                     /locus_tag="PKV56_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOX66925.1"
                     /translation="MVGQVKFNEVSKETLLEFWHGVVSKTGLKISYRERMVGIERQGD
                     LFVVQTDCGSYTARSVLLAMGRRGTPRKLDVPGEEASKVVYRLVDPEQYRGQQVLVVG
                     GGDSALEAAIALADQPGTGVILSYRSAAFSRVKGRNRDLLAAHEKAGRVQVLLTSSVK
                     TIEVRSVTISHEGADRAFPNDAVIVCAGGELPTPLLKKIGIEFETKFGSR"
     gene            complement(8530..9171)
                     /locus_tag="PKV56_03595"
     CDS             complement(8530..9171)
                     /locus_tag="PKV56_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX66926.1"
                     /translation="MRVLLVESDAVSGGNVRDLLRAGGHAVNWYRRLSDVTLLGEQAH
                     DVMLVALQLPDGSGLDWVDARRRNGDATPVLMLSDHCQPVDLWQPTDSSAEAREVLER
                     VLAAIRHALAQEPSADRRMRTYGHIELDPPSRRAFVRGSAVRLTEREWEVLAALTENP
                     GRLVAKRDLDALVGGPAEPASSNVIEVHVSSLRRKLGRSFIETVRGLGYRICI"
     gene            complement(9347..11089)
                     /locus_tag="PKV56_03600"
     CDS             complement(9347..11089)
                     /locus_tag="PKV56_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX66927.1"
                     /translation="MNQLKVSTRLLLLTGTLSLLLIVIGAIGLWGISDANQSLKIVNE
                     GRVAPLGALAEIQRRQLETEAMLINAIGDPRAEEIAKAQSELQENSVAIDALWKEYTA
                     RPLTVEETRLAQSFGDARARYQSGGVQPTMAALKAGRLKDATQMVDDVLSPLSEPVDK
                     NMQALLKLQIDVAREEYASAEAKYATVRAFAIAAMAAGLLFAAAFSSWMVRSLTRQLG
                     AEPAEAKAMAQAVADGDLTVRIDLRPGDTDSLMSSLNVMRDSLQRTVGEVRRNAEGVA
                     TASAQISQGNNDLSSRTEEQASALEQTAASMEQLTATVSQNADNARQANQLAISASSV
                     AVEGGQVVERVVDTMKGINDSSRRIADIIGVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRNLAQRSAAAAREIKDLITTSVERVEAGTQLVDEAGSRMSEIVTSIRRV
                     TDIMGEISAASAEQSTGMAQVGEAVTQMDQATQQNAALVEESAAAAESLKTQAQQLVA
                     AVALFRLRHGDVHPAPTARQVAAPPPKAYQTPERRSPHRATNVVRPAFNAAHTVPAAA
                     AAPAATGSDDWTSF"
     gene            complement(11281..13605)
                     /locus_tag="PKV56_03605"
     CDS             complement(11281..13605)
                     /locus_tag="PKV56_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66928.1"
                     /translation="MNQAAATEAARSDAGMSLTRRLFGMLVLVFVAVAAAFALFTRSV
                     IEHGLEHQRRDTGSHFEARIRALDQGWRQSAFSVRQQTELWQVDVERLPEVVAEARLR
                     ALMTTLLDQGEFTHAAIVGDAGQVLFRYGTRSQDLLTLPADSDAAGLGWVYSDRDRTV
                     YRTVTGPVRQAGRPAQLLLYVPLDNALLAQLAYPNTQLDLLHEGQAVATSDAGALMSG
                     PRASAVSADQRTNLSLRWDAGPGAPMLRIDRQFVTPISGLELLLIAGTCTGAYLVLGW
                     WLLIRWVRSQARRLAQLRQAATEFAATPVMSSDLELRLSAVADRGDDVGTLALTLGDM
                     MGRIEQGQREQARVQQQLAVVNAGLEDRVAERTRELEQARDEALAAARAKEQFLSNMS
                     HEIRTPMNGMLGALDLLSHTALNPRQAQYIDVAATSGEALLGILNEVLDFAKIGAGGL
                     QLAREPIDVNAVARSVTTLFSASAQRKSIELRLDSDPALDGWRYGDSLRLRQVLLNLV
                     GNAIKFTQRGCIVVGTHCIGSGVSEQVAFEISDTGIGIDKTQHDRIFEPFVQAQDPAR
                     PAQGGTGLGLAISRDLVRAMGGELVVDSLPGQGTVFSFALPLQRAPGPAAAAPAPAPV
                     NAVAPESRDERRPEGLSGRVLLVEDNSVNRMVGTAMLESMGLEVVVAEDGEEALAVLA
                     QTKVALVLMDCQMPVMDGYEATHRLRERERLSGGPRLPVIALTANALNGDIERCLAAG
                     MDAHLAKPFMISQLHALIEHWISAARAEEVVPGG"
     gene            complement(13602..14648)
                     /locus_tag="PKV56_03610"
     CDS             complement(13602..14648)
                     /locus_tag="PKV56_03610"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX66929.1"
                     /translation="MRTCPFLPRRLLLKRAALAAAALWASADVRAQAAAATEVVLATP
                     GPSSAVSLIPELAVRIGADRAEGLALRLKFVTGGGVAIREMASGNAQFGVFGVPAAMK
                     ENLESRRLVALAAIEDRVLLSVMVRSDLKDKVRRVEDLRGRVLGIHSNSLTSSTTGQQ
                     FLVLVLRQHGVSPESVRFVAAGQSWDTQASAMRAQVADAIVSEEPFALRLAQEGLAFP
                     LFRIGHPEDPRSLPGVGFLRGALISSVGLVEGRPELAQKMVRVVQRTLAWRRDHTPQQ
                     VVDALGLTGADALAIAEMLRQYPQQFSPDGRFSQAQIAQTETFFGESAGQAPEAVAYR
                     LPSMIVDRWAGRKP"
     gene            14858..15796
                     /locus_tag="PKV56_03615"
     CDS             14858..15796
                     /locus_tag="PKV56_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HOX66930.1"
                     /translation="MNFQQLRAVREATRTGFNLTEVAGVLHTSQPGISRQIRELEDEL
                     GIDIFVRAGKRLTRLTPPGEAVLPIVERMLLEADNLKRVGDDFNAQDTGRLSIAATHS
                     QARYALPTVVRDFRQIYPKVTLLLHQGSPKQVAEMLLSGEADIGVATEALAQYESLVA
                     LPCYRWTHSIVVPPGHELLDIDGPITLEHLARHPIITYETGYTGRAHIDDAFAKAGLT
                     PDIVLSAMDADVIKTYVELGMGVGIVASIAFDAERDRTLRALDARHLFEINLTRLAVR
                     RGTWLRGYVYAFIEAFAPTLSRPVVERALAGDVHAE"
     gene            complement(15818..16912)
                     /locus_tag="PKV56_03620"
     CDS             complement(15818..16912)
                     /locus_tag="PKV56_03620"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOX66931.1"
                     /translation="MTTLLYPATEPLAAQAPMPHRKLIRAWLTPICERSTVYALWLVL
                     FDLVLFAAGLVITVQASHPALQVLAGLATGFVIGRLFILGHDACHQSLTRHRTLNRWV
                     GRLVFLPSMTPYSLWDIGHNLVHHGYTNLKGFDFVWEPRTLQEFQALPAWRQRLERVY
                     RSGWAPWLYYLVEIWWGRMMFPSKEVMPTRRKAFLWDCIGVSIGAAVWIGGLVLAASA
                     TGQSPWWLVTVGFVIPFVFWNGMIGFVVYVHHTHSGVAWYADKASWASTSPFVSTTVH
                     LTFRSPMGSVLHHIMEHTAHHVDMSVPLYRLKRAQKLLETMLPGRIVIQPFSWRWYAD
                     TARRCKLYDYEARCWTDYSGRRTTDPVLVG"
     gene            17088..18212
                     /locus_tag="PKV56_03625"
     CDS             17088..18212
                     /locus_tag="PKV56_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOX66932.1"
                     /translation="MRHWVKLIAGQIGLHEPLPFGVHDENGKLLLARGQVIDNEAQMA
                     ALLSRGVFADEGEIEAARNGAPPEKAAKRLTMFDLWEQAIGRLDRLIKSVGQEPDFAG
                     RCDEFAGQLMGLVERDPDIGIYLSVRQDESRLHLYGLTHALHCALVCQLMALRIGWPV
                     DRTRRLVKAALTMNLSIMEFQGRYAVHGRFDDEQKAQLRLHPAQAVERLRAAGIDDEI
                     WLQAVMEHHERPGGGGYPNDLVELSEEAGILRMADIFMAKISPRATRAPLSIQEAARQ
                     MYQEAHGSAAAAAIIKEYGIYPPGNLVHLASGELAIVIRRGATAHTPVAAAITDKSGV
                     PTISTTRRDTAQPAFAIKALEFDRSLALRVPPERLYGLAE"
     gene            complement(18225..19283)
                     /locus_tag="PKV56_03630"
     CDS             complement(18225..19283)
                     /locus_tag="PKV56_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOX66933.1"
                     /translation="MSIEVRNLNKRFGKTVVCDNLNLDIPSGELVALLGPSGSGKTSL
                     LRIIAGLEVPDSGTVLFHGEDTTYTDVRERQVGFVFQHYALFAHMTIFENVAFGLRVR
                     PKATRPSEAQIRAKVTDLLKLVQLDWIADRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDEVHVTSVFVTHDQEEAMEVADRIVVMNEGRIEQV
                     GSPDEVYDHPATPFVLKFLGDVNLFHGRLDHAQGAVRDAADVSYVRPHELQIVATAQE
                     GAVAVTLSQALTVGPNTRLEFRRLDDGSFIDVELPRAEFRQLRERMTLEPGTTVHLVP
                     RRITRFAEEQQPDPAAAI"
     gene            complement(19695..20570)
                     /gene="cysW"
                     /locus_tag="PKV56_03635"
     CDS             complement(19695..20570)
                     /gene="cysW"
                     /locus_tag="PKV56_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOX66934.1"
                     /translation="MHAGRASAPPSVRAATTEPTWVKWTLIGVAMAFLTFFLFIPLTI
                     VFVEAFKKGVDVYLASITEPDALSAIKLTLIAAAISVPMNLVFGVAAAWCIAKFEFRG
                     KNVLLTLIDLPFSVSPVISGLIYVLIFGLQGWFGEWLRDHDMKVIFAVPGIVLATVFV
                     TFPFVARELIPLMQAQGTEQEEAACVLGASGWQIFWRVTMPNIKWALLYGVILCNARA
                     MGEFGAVSVVSGHIRGQTNTMPLHIEILYNEYQFAASFAVASLLALLALVTLVLKYVV
                     EQRVKAQKRAVDERA"
     gene            complement(20620..21453)
                     /gene="cysT"
                     /locus_tag="PKV56_03640"
     CDS             complement(20620..21453)
                     /gene="cysT"
                     /locus_tag="PKV56_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOX66935.1"
                     /translation="MFLSRTLLRRHSVLPGFDLALGFTLLYLSFIVLIPLSAAFLKTA
                     TMTWPAFWDAVTSPRVMASYRLTFGASLAGALLNAFFGLIIAWVLVRYEFPFKRVVDA
                     LVDLPFALPTAVAGIALTALYAQNGWIGQWLPFKVSYTPLGVFVALTFIGLPFVVRTL
                     QPVLEDLHKEVEEAAATLGANRWQTFTKVIFPILTPALITGFALAFARALGEYGSVIF
                     IAGNMPLVSEITPLLIITKLEQYDYQGATAIAVVMLVAAFSLLLAINALQAWTRARQG
                     R"
     gene            complement(21466..22488)
                     /locus_tag="PKV56_03645"
     CDS             complement(21466..22488)
                     /locus_tag="PKV56_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX66936.1"
                     /translation="MTPVSRRAVLVTLASTAALTSFTASAQGAGKTLLNVSYDVSREF
                     YKEYNAAFAVHWKKTTGEELTLNQSHGGSSKQARSVVEGLEADVITMNQANDIDILAD
                     RGALIPADWAKRLPDNSAPTTSLSVILVRKGNPKNIRDWSDLGRPGISVIIPNPKTSG
                     NGRYTYLAAWGAGLKNGVAPAAAKALVERIFANVPVLDGGGRGATTTFAQRNLGDALV
                     TFESEVPLIKREFGGDYEVIYPTWTVLAENPVAVVDKVVDKRGTRKQAEAYLKYLWSD
                     EGQDIAARQHIRPRSAKVLAKYAKDFPKVNAFTVDEVFGGWKKAQKEHFDDGGFYDQI
                     VLRSKK"
     gene            complement(22683..23696)
                     /locus_tag="PKV56_03650"
     CDS             complement(22683..23696)
                     /locus_tag="PKV56_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX66937.1"
                     /translation="MSASSFTRSALLVLAVSAAAFGAAAKDVSLLNVSYDPTRELYVE
                     YNAAFARYWKARTGDVVTVRQSHGGSGKQARSVIDGIDSDVVTLALAYDIDEIAAKAK
                     VLPADWQKRLKHNSSPYTSTYIFLVRKGNPKGIKNWDDLIKPGVGVITANPKTSGGAR
                     WGYLAAYGFALQQPGGSDAKAQQFVASLFKNVPVLDSGARGSTVTFAERGIGDVLLAW
                     ENEAHLALKEFGADRFDIVYPPVSILAEPPVSVVDKVVDKRGTRDVAQAYLEYLYSAE
                     GQEIAARNFYRPIDPAVATKYEKNFPKINLFTIDAVFGGWAKAQKTHFADGGVFDKIY
                     TVK"
     gene            complement(23987..25741)
                     /locus_tag="PKV56_03655"
     CDS             complement(23987..25741)
                     /locus_tag="PKV56_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX66938.1"
                     /translation="MNQLKVSTRLLLLIGTLSLLLVVIGAIGLWGINDANQSLKTVNA
                     DRVVPLGQLAEIERRMLKSQAALLNAIGDPRPEEIATATTTLPDNAAAIDALWKAYTA
                     TFLTTEEARLVQAFGDARARYRSEGLQPTLVALKEGRVKDAGKMVDELLNPLYKPVEE
                     NLEALLKLQNEVAAEEYAAALSSYATIRAVALGSIVAGLLFAAVFGTWMVRSLTRQLG
                     AEPADAKALAQAVAEGDLTVQIDLRPGDTDSLMASLNAMRDSLQKTVGDVRRNASSVA
                     VASSEIAQGNLDLSQRTEEQASALEETAASMEELTSTVRQNADNARQASQLASSASEV
                     AVRGGEAVGRVVETMKGINESSKRISDIVSVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRNLAQRSAEAAREVKTLINDSVGRVEAGSAQVDQAGATINEVVVSIRRV
                     TDLVSEISAASAEQSAGVAQVGEAVAQMDQVTQQNAALVEEGSAAAESLRVQAQQLVQ
                     AVSAFRTESNGGSMVAAAQAETAGWGGEERRGPNRARNVVRPTFTPRKAAATASQSSA
                     AQRQQPLKTGTDDSWESF"
     gene            complement(25767..26279)
                     /locus_tag="PKV56_03660"
     CDS             complement(25767..26279)
                     /locus_tag="PKV56_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX66939.1"
                     /translation="MQTLTSPNRTEVVRPEPGDSPEGQRVLAFKIGSQSYAVDILQVC
                     EIRSYSAPMKLAHSPCDQLGVMNLRGEMVPVVDLRLRLGAVACEYDAQKSVIVVERDG
                     KRLGAVVDSVSDVLDLAAEHLRPVPSMPGFSSSGHLRGIATIDDQMLVLVDIASLVGS
                     AAGVAVAALH"
     gene            complement(26468..27238)
                     /locus_tag="PKV56_03665"
     CDS             complement(26468..27238)
                     /locus_tag="PKV56_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66940.1"
                     /translation="MALPRVLLVEDDVSIRRLVEMSLDGMPIELITCEGAAQARERLR
                     ESPVRLLITDLMMPGETGLELLQSLERDPALRAGARLVVFSAGISPELERTMAALGVW
                     RFLTKPSSMLVLERCVLDALGLDLADDPAARAVPAADETAAVPVVDVHEQAAIDRHFA
                     GNAPLFHAFRATCLTQFADDLQEGDRVAGARNAQGMRRLAHSLKSVLRTLGHEAEADG
                     AARLESAAAAGDWTAAAAPWADLRAVLQRLSSDASTGG"
     gene            complement(27248..27946)
                     /locus_tag="PKV56_03670"
     CDS             complement(27248..27946)
                     /locus_tag="PKV56_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HOX66941.1"
                     /translation="MTAHTTDFSGQDTAPDDVSTTIEVAKQLGLAVRSVQLMVDRGEL
                     VAWKTAGGHRRISRASVQRWLAQRQAGGAAVDAHAAAPARPETAPNTTPTSSERRGAP
                     RVLLIEDSRHYQNLIKLLMQQQFPQAQLSVADDGIAGLAMAGQLQPEVLLVDILLPGI
                     DGATLITGLRSHRTFAGSQLIVITSLDGAELEPYAFALTGVPVVHKPKLVVELPALLN
                     AALQAAAATPCPAA"
     gene            28055..28426
                     /locus_tag="PKV56_03675"
     CDS             28055..28426
                     /locus_tag="PKV56_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66942.1"
                     /translation="MKKILIVEDHADIRRLIRMTLEFEECEIHEAPDAATGWDMAREL
                     QPDLVLLDVMMPGDLDGLGLCRAMQCHSRLRDVPVIMLSARGSSADREAGLQAGATAY
                     LVKPFSPMQLLTLLGSTATAH"
     gene            28437..29225
                     /locus_tag="PKV56_03680"
     CDS             28437..29225
                     /locus_tag="PKV56_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOX66943.1"
                     /translation="MNAMTEAASLAGSNCFEDAASAQMERLVADFGRMYHERNEALQE
                     VARAHHDALLRLSRAAEFRDDDTGVHIVRMGYLAWALALRLGQTEQYAMLLRQTAPMH
                     DIGKIGIPDAVLKKPGSFNPEERAVMNQHPQIGAEILGRSRIPLFTAAAEVALSHHER
                     WDGSGYPSGMAGATIPLSGRIVAVVDFFDALTMDRCYRPAFEDHVALEMLIEQRGRAF
                     DPLVVDTFVEHATDLIELRDQVNLQKLDFEDLCSGGDPFVRRGD"
     gene            29230..31773
                     /locus_tag="PKV56_03685"
     CDS             29230..31773
                     /locus_tag="PKV56_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66944.1"
                     /translation="MVRRLKRRAKPTDVGLAILTGIVVTIFLWSALVGFALHERRQAI
                     AEAHNHTGVQSRAFSDGIRRSIDSAILANTAIGNQLELGLPADGESTRSAIAQTATSL
                     PFLLGLGIIDAQGLVIGGTDLAAINRVIDLKALGTLPSVDQDRLGEAVAAGSLLDLAP
                     ASSPRRTQPGDSLLPLLRTVQLPSGQRVHLIAMLDGQAFAAVQRDMMSDSSLLATLAS
                     SDGRLIASTAVTARAAGSSIKDVPLFQGLLQKQPQGEWTGAGVRGTEQIASFSSIAGH
                     PLVAMVETSQHDAMADWLKKERLLIIAGLVLTLLTAGGTWIVMRSLRARMAARLEIDA
                     AHLRASQRETELTVTLKSLQELIFRTDANGAITFVNNHWMPVMGTPVETAQGARPWEL
                     VLARDRDAVWDLFATRPGLEARRAQASLVIATGVVRTFDFSVTPLQHEGVVTGFAGSA
                     IDVTERVAAERQLEAQLAFTRLVMELSPLPKSVVSMKGRHLIVNKAWEEFTGQQSGDI
                     AFETQDDSEQATPRPLFEARDRKRLAPGEPLRYETTVKHRDGSTRDVMVNKVMLHGPD
                     GRPAGILCVFMDISEFRNAERATREARDIAEEASRAKSEFIANISHELRTPLQSIIGF
                     SELGQMRARPNERMAAMFDDIHGAGQRMLTLVNDLLDVSKIDSTVGAIHLETNDLRGL
                     VRGVIREIHPLLAARRLQLVTHLPDEPMLARVDPPRFQQVVRNVLANAIKFSPAGSRI
                     DLVGDHTPSGELRLVLRDQGPGIPVAELETIFEAFVQSSHTKDGSGGTGLGLAICRKI
                     IDAHGGRIHAQNRPEGGAAFHVVLPARPAPTIDESEPESEAATTCRLAA"
     gene            complement(31840..32214)
                     /locus_tag="PKV56_03690"
     CDS             complement(31840..32214)
                     /locus_tag="PKV56_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX66945.1"
                     /translation="MKSILIIEDQPDIRRLIRWALEFEHYAIHEAANGAAGLEAARNL
                     KPDLILLDVMMPGDLDGLQVCAAVKADPVLAHVPVVLLTARAQDSDREAGQRVGANAY
                     LVKPFSPLALIDTVNSLIVPGP"
     gene            complement(32211..34505)
                     /locus_tag="PKV56_03695"
     CDS             complement(32211..34505)
                     /locus_tag="PKV56_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX66946.1"
                     /translation="MKLPVRWLPRSLVGRVFALYTVTLLAFVLGGLGLFYSYQFSVEL
                     EEAQLRADTLSGVIMPTISDSAVIGDYDTIRRTLESALLHSSFSVASFIDLKGGIVRA
                     PRNDPPDVSAPGWLVDRVASRLYDTNQTVTVGGRDYGVLRLTFSPERIAGRLWEQTRM
                     ALVLALLSLAGGLLLIRFPLVRWLGNLGRIQEFERAMQAGEATPEMLMPEDAPTEFRA
                     TFEVIGRTVQSLRAQRTQAAVTLSAIADSVFTLDAQGRVVFANPAACALVDRPLGELL
                     GESMTEVLPAVYRHVDPLSSWTGRRATLSASPGREVVVDTTQSVILAPDGEIAGHVLA
                     CRDMSEQHALDQRLRAELQSRAMALISLRQVLEGLIRDTSVGVEGADDIEAVSMMIFE
                     LVSRLQQRGEQLDAIFALSPDGFVSFDSQRRANYMSPAFSRLTGLTEQQILGAEEGTI
                     EAMLRERVTATATWRGFESMRRDLRDAVGEAQPRRELIDLAWPKPRVLEAGLCLGSTE
                     AISQVLSLRDVTHETEVDQMKSEFLSTAAHELRTPMASIYGFSELMSRRKLSPEQQAE
                     VISTIHRQSELMIAIVNELLDLARIEARRGKDFTYEVLDLATLLPELLQEFKPPDDRS
                     PAQLAPLSKSIIVRVDRNKMRQAIGNVVANAYKYSPQGGAVEVRLVHGDSEPSMVGIE
                     VRDHGIGLTPEQIARVGERFYRADASGNIPGTGLGISIVKEIIELLGGRMAIASEPGH
                     GTTVTLWLPTTPESAPVEAALEHA"
     gene            complement(34518..35372)
                     /locus_tag="PKV56_03700"
     CDS             complement(34518..35372)
                     /locus_tag="PKV56_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX66947.1"
                     /translation="MHTHFLARLAASCMAGLLFLAGPARAQEDAYRFSPVNQYGINLT
                     AAYWNPIIAYVSEKSGVKLQLKIGRTSADTTAYVLAQEVEFVFSNHLFSPEREQLGWK
                     VFGRRATPPMHAQIIVPTDSPITELSQLAGKEVVFPGPEALVAYKFPMAQLMARKIDA
                     KAVFGGNMDGALAQLFSGKVAASGANSQLAEGYAKREGKTYRVLWSSPPVHDLALMAS
                     SKVPEKIQKAVAGAFIGMSRDPKGREILAQASAEVGLTVDANFIASDGSEYATYRDFF
                     RTAPPSLR"
     gene            complement(35525..39664)
                     /locus_tag="PKV56_03705"
     CDS             complement(35525..39664)
                     /locus_tag="PKV56_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX66948.1"
                     /translation="MLSARDRDNEAACLAALDALEVLDTAPEPEFDALTRAAAMLCAA
                     PIALISLADRGRHWLKAKVGLPDLTEVPRSIGLCSYAILCDDLLEIPDTLRDPRFADN
                     PLVTGEPGIRFYAGVPLRLADGLCVGTLCIIDRQPRQLDETQRELLRCLAQVASRALQ
                     ARLTLQGAQQTLDWLTASEARFRVLSESSPLGIYYTDVHGACCYTNARWQQIFGLTLA
                     DSLGHGWLQAIHLADVEQVQAEWQRFSADGSEFDLQFRVVRPDGELRRVHSRARSLHD
                     ASGARTGYVGSAEDVTAHHDLLAQLSLNEERLRRLYEATPAMLQSIDGNGRLLSVSDA
                     WLTKMGYTRDEVIGHPSAAFLSSASREHALNVVMPAFFQSGRVDNIAYQMVTRSGGVI
                     DVRLSAILERDVHGRPVRSFGVIEDVTLQRIAERALSTERQRLTNLVEGTHAGTWEWQ
                     VQTGEVHFNERWAQILGYTLAELEPTSIQSWMARGHPDDMVRSTEILARHFAGQIDHY
                     ECETRRRHRDGHWVWVLARGRVITWTPDGKPEWMYGTHLDVTARKLKEEALRKSERFL
                     DRTGKMAGVGGWEVDLPSGDILWSDETCRIHGVSPGYQPTLDEAIAFYAPEARDAMRV
                     AVEVAMSGGAPFELELPLIRRDGRSIWVRVVGSLEIADGKPRRLVGAFQDVTQQVAER
                     HALSTARERAAVATDSGGIGIWDWDLRTRALVWDARMFRLFGSFQDETADPWHIWCQR
                     VHRADMPAVDRGIQQSIDEDTPFDTEFRIVWPDGSVHHLRACARVTRDADGTALRMVG
                     TNWDVTEQRRLAAELAEQHERLRVTLRSIGDAVITTDPEGCVSWLNPVAEKLTGWTTA
                     EAQGQSLSRVYRTIDEESGEAVESPVTTCLTKADAPSPVLKTLLICRDGEQLAIEDSA
                     SPICNDQGQVLGVVLVFRDVSAQRQMVGEISYRATHDDLTGLLNRAAFESRLGSVLKK
                     SRDDGGENAVMFIDLDQFKLVNDACGHAVGDQLLQQVSRLFGNVIRASDTLARLGGDE
                     FAIILERCTAEQAQRVAQKICNQMDDYRFVHDGRRFRIGASIGLVPVDQRWSSISAIL
                     QAADTSCYAAKEAGRNRVHVWFDSDAAMHARHGEMQWTNRIELALDHDGFVLFAQRIE
                     SLGEARSGVHAEVLLRMVGSDGSLIPPGAFLPAAERFHLASRIDRWVLSHAIGWMTSL
                     PDLTAIDTLSVNLSGQSVGDRAFHRWAVDLLTQAGAEVCQRLCLEITETAAVTHLADA
                     GAFIEQVKAFGIQVALDDFGAGASSFGYLKRLPVDVLKIDGQFIRDLVGDPLSMAAVR
                     CFTDVAKVMGVKTVAEFVETAEVMQQLREMGVDFAQGYLIHRPEPIDRLLQSIEAPLP
                     A"
     gene            complement(39681..>39882)
                     /locus_tag="PKV56_03710"
     CDS             complement(39681..>39882)
                     /locus_tag="PKV56_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HOX66949.1"
                     /translation="DIVAEISAATTEQSTGISQVGQAVSGMDQTTQQNAALIEQSAAA
                     TESMSKQAHELVQSVAAFKVRV"
BASE COUNT         6933 a        13473 c        13058 g         6418 t
ORIGIN      
        1 gactggaaaa agggctctcc gcgcgataac cgaatcctgt tcatcggccg agacatgccg
       61 caggaggtca tcgttcaagg actgaaagac tgcctggtct gcgcaggcgc cactgccgcg
      121 ccgccccgcc gagcagcgat caagcagggt tcgaccgtgc gaccgccgtt caggagagtc
      181 gcatgagtct gatacccgtc accatactca cgggctttct tggcagcggc aagaccacgc
      241 tgctcaaccg catcttgcga gagcggcatg ggcagcgcat cgccgtgatc gagaacgagt
      301 tcggcgaagc cggtatcgac aatgagctgc tggtgcaaga cacgggcgag caaatcatcg
      361 agatgaacaa cggctgcatc tgctgcaccg tgcgtggcga cctggtgcgc atcctcggcg
      421 agttgcacgc caagcgcacg gcaggaaccc tgcacttcga cagcgtgatc atcgaaacca
      481 ccggccttgc cgacccggca ccggtggcgc aaacgttttt cgtcgacgaa gacatcggcg
      541 accactaccg tctcgatgcc gtggtgacga tggtcgacgc caagcacgcg atggcgcagt
      601 tcgacgcgca ccacgaagct caggagcagg tcgggtttgc tgaccgaatc ctgatgacaa
      661 agaccgatct cgtcagcgtt gaggaattgc gtgcgctgga acaacgtgtc aggcgcatga
      721 acccccgtgc gccaatagcg gcagcccatt tcggcgtcac gccgatcgaa cagttgctgg
      781 acgttcgcgg gttcaacctg aacgacgtcc tgcagatcga acccgacttc ctgaccaatt
      841 cagatcatga acacgacgac gaagtgacga gcttcgtgtt cagcgcggag caggctttca
      901 tcgcggagcg actcgaagac ttcctgacgg ccattcttca gctctacggt gcaaacctga
      961 tgcgctacaa gggcgtgatc gatgtggaac accacgatcg acgtgttgtc ttgcagggcg
     1021 tgcacatgct gatgggcatg gattccggct ctgcgtgggc ccgcgatgaa caccgcaaca
     1081 gccgcatcgt cttcatcggc aagagcatgc ccaaggacct gatcctgcaa gggctggaac
     1141 agtgcctggt gtcagccaaa acggccgctg caaaggtgca ggaaatcaca tgaacgaggt
     1201 gaccgtctac cgccagttca gactgagtca ggccagacag aacctcttcg catgattcga
     1261 aagaacccac gaggcgatct gccccaggcg catgagagcg cgttcgtgga cccgaccgcc
     1321 ctcctgtgcg gccgggtggt ggtgcacgag aacgtcttca tcgggccgta cgcggtgatc
     1381 cgcgccgacg aggtcgatga cgaagggcac atggagccca tcgtcatcgg cgcccattcg
     1441 aacatccagg acggcgtggt cattcactcc aaatcgggtg ctgcggtgac catcggtgaa
     1501 cgcacctcga ttgcgcatcg agcgatcatc cacggtccgt gccgagtggg caatggcgtc
     1561 ttcatcggct tcaacagcgt gttgttcaac tgccggatcg gcgagggctg cgtggtgcgg
     1621 cacaacgccg tggtcgatgg ctgcgacctg ccaccggggt tttatgtgcc atccaccgag
     1681 cggatcggcc cccacaccga cctgaatgcc gtggcgaagg taaacgtcca ggcttcggag
     1741 ttttccgaag acgtggcgcg cgccaacaac gatctggtgg ccggctacaa gcgcattcag
     1801 aatgaacttt gacccctccg aaaggttgac atgaaagaga ctatccaact cagcctgctt
     1861 cgctgtgtgc tgacggctgg agctttggcc atggctatcc aggccaacgc aaccgaagag
     1921 gcccatgtcc atggcctgat gcatatcaac gtggctatcg acaaacaagt gctgacgatc
     1981 cagctcgaat cgccgctgga cagcttactg ggcttcgaga acaagcccaa gacaacaacg
     2041 cagcggctgg ctgtcgatgc catgtcgaag cagatgaacg acggtgcagc actgttcaaa
     2101 cccagtatcg cagcgcagtg caagctgacg aaggcgacgg tcgaatcggc agtcttccag
     2161 gccaccgctc ccgcaggcga caaggaagaa ccgcatgccg acctggatgc cagctatgaa
     2221 ttcacatgtg cacaacccga caaactgacc agcgtcgaga tcgggttgtt ccaagcgttt
     2281 cctcgcctgc agaaaatcga ggtgcaggtg gccgggccga agaagcaatc gaagacgtcc
     2341 ctgagtcggc cggcgaacac agtcaagctg aagagctgac tgccggaatg tggatcagaa
     2401 cccagtcgct gacaaaaccc tatgtgaggg catggcaaat catcgccaag agagagcctc
     2461 ggccgggagc gaccagtcct ttcgggcagc cgatcaaccg cccaggctca gcgccacgcg
     2521 ataggtgatg tacgacgcgg cataagccag tgcgaacagg tagcccgcca tcagcagcgg
     2581 catggtccag cctcccgtct cgcgcttgac cgttgccagc gtggacaggc actgcggagc
     2641 gaacacgtac caggccagca gcgacaacgc cgtggcaagg ctccaggttt gcgagatcag
     2701 cgggaccagc gcctgggcgg tgtcttcgcc ggtggccgac agtgcgtaca ccgtgccgag
     2761 ggcaccgaca gccacttccc gagcggccaa cccgggcacc agcgcgatgc tgatctgcca
     2821 gttgaatccg atcggctcga agaccaccgc cagcgcatgg cccagcatgc ccgccacgct
     2881 gtactcgatc ggagcgcctg tcgcaccagc gggcggcgct ggaaagctcg cgaggaacca
     2941 cagcgcgatc gtcaggccga ggatgatgcc gccgacgcgt ttcatgaaga tctccatgcg
     3001 ctgccacagg ccgatggcca ggttgcgcaa tgtcggccag tggtaggcag gcagttccat
     3061 catcagcgaa cgcacctgcc cacgtgcggt gaagcgcttg agcacccagg ccacggccag
     3121 ggcgcctgcg atgccggcca cgtacaaccc gaacagcacc agaccctgca gctggaatcc
     3181 gccccagacg gagcgagccg ggacgaacgc gccgatcagg agcgcgtaca ccggcaggcg
     3241 ggccgagcac gtcatcaggg gcgcgatcat gatcgtcacc agccggtcgc gcgggttggc
     3301 gatcgtgcgc gcggccatga tgcccgggat cgcacaagcg aagctcgaca gcagcgggat
     3361 gaacgagcgg ccgctcagtc ccacgctgcc catcagccgg tcgagcagga aggccgcgcg
     3421 cggcaggtag ccggactcct ccagcagaag gatgaagaag aacaggatca ggatctgcgg
     3481 caggaacacc accacgccgc cagctccggc aatcacgcca tcgacgacca ggctgcgcat
     3541 ccagccatcg ggcatcgccg acgtgaccgc cgcccccatc cattccgtgg cgctcttgat
     3601 cgcatccatc ggcacctgcg cccaggcgaa taccgactgg aagacgagga agagcacgac
     3661 cgccagcagg atcggcccga agaccggatg cagcaccacg cggtcgatgc ggtcgctcgc
     3721 cgtgtgcggc aggacggcgg ccagcccgag gcgctccagc atcgagtgca ccacttcatg
     3781 gtcggcttgc gtgacgatgg cggactcggt ggcgccatgg gctgccgggc ttgatacgcg
     3841 ctgccagacc agcggatcgt ccagcatggc acgcaggcga ccggcgccct cgttgcggat
     3901 gcccaccgtg gcgaccaccg gcaagccgag ctcggccgac agcgcctgcg cgtccacatt
     3961 gatcccgcgg cgtgcagcca gatcggtcat gttgagcgcc acgatgcaag gcagaccgag
     4021 gcgcttcacc gccatcacga tgcgcaggtt gcgacgcagg ttggtcgcat cgaccacgca
     4081 caccaccaga tcggggcgct tctcgccctt ggcacgtccg tacagcacat cgcaggtcac
     4141 gcgctcgtcg ggcgaccgcg gatgaaggct gtaggcaccg ggcaggtcga gcacccgcaa
     4201 ggtcttgccg gcgggcgtga tcaggcggcc ctccttgcgc tcgacggtca cacccgcgta
     4261 attggcgacc ttctggcggc ttccggtcag caggttgaac agcgccgtct tgccgcagtt
     4321 gggattgccc accagcgcca ccagcggccc gccatggtga aagtggatga cggattcgct
     4381 cacgccgaag cctcgaccac gacgcacgcg gcttcggccc tgcgcagcgc gaaggtcgac
     4441 aacccgactc gcacgaccag cggatcgccc ccgaaggcac cgcgcgccag cagctcgacc
     4501 tgctcacccg gcatgaaacc gatctcctcc agccactgcg cccactccgg ggcgcggggc
     4561 ggcgcctgca cggccctgac gaggaagcgc tggcgcaacg gcgtgcagtc cagtgtcaac
     4621 aaaggcttcg agtggttcac ggcaactgcg aggaatgaga agagccgaat cggtcagtga
     4681 tccggcacac aggcaccgag gttacaccgc atccaatgca aacacaaata cgacccgttc
     4741 gcatttgttg ggagatcatg gacccgttgg accggacggc gtcagcggcg cgccgccctg
     4801 ccgctggccg gcgccggggc aggcccgggc tccccgctcc cgagatgaca atatcgcgat
     4861 gggcccacct tccatcatcc tgatcgccgc tgtcgctcgc ggcggcgcca tcggcaggga
     4921 caaccagttg ctggtccatc tgccgggcga tctgccccgc ttcaagcgat tgacgatggg
     4981 acacccgatc gtgatgggcc gcaagacctg ggactcgatc ggccggcccc tgcccggtcg
     5041 acgcaacatc gtcatcaccc gacagcccga ctggtgcgca gccggtgccg aacgcgcctg
     5101 cggcttcgag gccgcgctcg aactggccgg cgatgttgaa caggtctgcg tgatcggggg
     5161 cgcggaaatc tacgcgcttg ccctgccccg cgccgatgaa ctgctgctca cggagatcga
     5221 tgccgattac gtggccgatg cctattttcc cgactgggag cgcggcgatt tcgacagggt
     5281 cgagagcgaa agccacgcca ccgaacaagg tctggtctac agctatgtca ggtaccggcg
     5341 caagtcacac cgctgaatcg gccagcggct tgcgccccgc cgatgccgac cggcagctca
     5401 ggtcgacttc atgtcgcgaa ggcacagtgc gtgcctcacc gccagaacgc cacccgcaca
     5461 tgccttcatt cattcgcccg gcacttctct cgctagcgat gttgctgagc ggcggcacca
     5521 tggccagccc ggacaaatgc aatgccggcc cgcgggacca gtggcaaccg atcgagacac
     5581 tggagaagcg gctgcgatcg gaaggctgga cgatcaggaa gtcgacgatc cacagcggct
     5641 gctacaagat ccgaggcaag agcggcaagg gcatgcgcat gaaggcctac ttcaacccga
     5701 agacgctcga gatcctgaaa gatgaatgag ccagccctgc cgatcgcacg gggactcctc
     5761 atggccagat catgagattg ctgcttctgg aagacgacgc gatcctcggc gaggggctgc
     5821 gtgatttcct gcagaccgat ggtcatcgag tcgattggtg caagcgcctg tgcgaagcga
     5881 aggcgctgac cggtgaaccc tacgatgccc tgctggtgga ctggcagttg cccgacggct
     5941 ccgggctcga ctgggtgcgt tcgatccggc gccagggcat ctcgacaccg gtggtgatca
     6001 tgacggcgcg tgacctgctg agcgaccgcg tgcgcggtct cgatggcggg gccgacgact
     6061 atctcgtcaa gccgttcgag ccggaagagt tgactgcgcg cattcgggcc gtccggcggc
     6121 gcgtctccgg atcggccagc gcgttgctgg cgttcggcga cgtccggctg gacctgaccg
     6181 gcaaggtcgc cttcctgcac gacgtgcgtg ccgacctgac ggcgcgcgaa tgggcggtgg
     6241 tggaagcgct ggccctgcgc gctggccgcc tcgtgtcgcg cagcgagctc gaggcactcg
     6301 tgctcggcct cgacaacgag tcggccagca acaccctcga ggtgcacatc tccagcatcc
     6361 ggagaaagct cggccgcggg gtgatcgaga cgtcgcgcgg tctcggctac cgtctggcga
     6421 catgacgccg aacaccagcg ccctgccctc gatccgccag cgtctgtcgc gggcgctgat
     6481 cggcgtgtcg atcggctggg gcatcgcggt gtcggcggtg gtctggttcg cggtgcgcca
     6541 tgaagtcgac gacctcctcg acaacgcatt gcaggaatcg gccgagatcc tgttcgcgct
     6601 gctgtcgttc aattcggcgc agctgtcgct caacggcggc ggcggctggt tgccagcgcc
     6661 ggtgcatgac gaggaactgg tctggcagat cgtcggcgcg gaccagcagg tcctgctgcg
     6721 ctctcaccgt gcgccagccg cgccgctggc caaccggcgc acgaccgccc tgtcgagcgt
     6781 cggggatgaa tggcgcgtct acagcctgcc cttcgacacc acggggcgca tgctcttcgt
     6841 ggcccagacc ggccgcagcc ggcaccaggc ccggctcgac gcagcgatgt tcaccgccag
     6901 cgccgcgctg ctggtgggcg tgctttgcgc atacctgctg cgctggcggg tccgccggga
     6961 actggtgccg atcaccgacc tgtctcaggc cgtgatgcag ttcgacccga tgcggcccga
     7021 atcgcggctc gaccccgccc ggcgcgccga gctgatgccg atgcacaccg ccatctcgga
     7081 gctgggcaca cggctggccc gtcgactcgg caacgagcgc gcgttctcgg cgcacgcagc
     7141 ccatgcgctg cgcacgccgc tggccggcat ggtcgcgcag ctggccgtcg cacaacgggt
     7201 gtcgtctgcc gaagcacagc cccacctgca gcgcacgcgc gaagcggcga gccggctgca
     7261 gcgcgtggtg accgcgctgc tgaccctgtt cagaaccggt ggcgaagtgc gcaggcagag
     7321 ggtggagatc gctgacctga tcgggcagtt gtccttcgag cagagcgtga tctcgtccgg
     7381 cggaagcacc cacctgcagg tggatccgga tctgctggca gctgcgctga tgaacctgat
     7441 cgacaactcg ttgcgccacg gcgccagcac agtcagtgtg cagtcccggg aggagcccga
     7501 cggccgcatc gtgatcgccg tccacgacga cggcagtggc ataccgcagg ccgatcgcac
     7561 tcgactgcag tcggcactcg acgcacagca gtacgacggc gggatgggac tcggcctgat
     7621 gctggcggac ctggtggcgc gagcccacgg cggtcagctc cgcatgaaga gctccgcttc
     7681 aggctgcacg gtcgagctgg acctcggcat ccccgcagca gactgagctg cgccgcacgg
     7741 atcgacgctc agcggctgcc gaacttcgtc tcgaactcga tcccgatctt cttcaggagc
     7801 ggcgtcggca gttcgccccc ggcgcagacg atgaccgcat cgttcgggaa ggcgcgatcg
     7861 gcgccttcat ggctgatcgt gacgctgcgc acctcgatcg tcttgacgct ggaggtgagc
     7921 aggacctgca cgcgcccagc cttctcgtgg gccgcaagca gatcgcgatt gcgccctttc
     7981 acccgcgaga acgccgcgct gcggtaggac aggatcacgc cggttccggg ctgatcggcc
     8041 agcgcgatgg cggcctccag cgcactgtct ccgccaccga ccaccagcac ctgctgcccc
     8101 cggtactgct cgggatcgac gagacggtag acgaccttcg atgcttcctc tccgggtacg
     8161 tcgagcttgc gcggcgtgcc gcggcgaccc atggccagca gcaccgagcg cgccgtgtag
     8221 ctgccgcagt cggtctgcac gacgaagaga tcgccctggc gctcgatacc caccatgcgc
     8281 tcgcgatagg agatcttcag gccggtcttg ctgaccaccc cgtgccagaa ctcgagcagc
     8341 gtttccttgc tgacctcgtt gaacttgacc tgcccgacca gcggcagcta ttcgaacgta
     8401 gagcggacat cggcaaatgc taagaagcca agagacctca gaaggtcttt tggcgcttca
     8461 ggtttgttgc gaggcgttgt tacaactcgc ccgacggacg agtcgttcgg agccgcggct
     8521 ccgatcgggt cagatgcaga tccggtagcc cagtccgcgc accgtctcga tgaaggaccg
     8581 gcccagcttg cggcgcaggc tcgacacgtg cacctcgatg acattgctcg atgcaggctc
     8641 cgcggggccg cccaccagag cgtccaggtc acgcttggcg acgaggcgac ccgggttctc
     8701 ggtcagggcg gcgagcacct cccactcgcg ctcggtcagc cggacggctg acccgcggac
     8761 gaatgcccgc cgcgaggggg ggtcgagttc gatgtgcccg taggtgcgca tccttcggtc
     8821 ggcggagggt tcctgagcca gcgcgtggcg gatggccgca agcacccttt cgagcacctc
     8881 tcgagcctcg gcgctggaat ccgtgggctg ccacagatcg accggctggc agtgatcgct
     8941 caacatcagc acaggcgttg catcgccgtt tcgccgacgg gcgtccaccc agtcgaggcc
     9001 cgatccatcg ggcaactgca gggccaccag catcacgtcg tgcgcctgct cacccagcag
     9061 agtcacgtcg gagagtcgcc gataccagtt gacggcgtgc ccgcctgccc gcagaagatc
     9121 gcgcacgttc ccgccactga ccgcatcgct ctcgacaagc aggacccgca tcttcaaacc
     9181 ctttcaatgg gtgagaaggt agcgacagtc ggcactcctg ggcttcaggt tcggttcagc
     9241 ttcgacgccg gtggcgcggg ccgctggagc aactgcactc gatatgccgg gcacgcgcag
     9301 gggcgaatgc ggcgccgggc gcctcattcg aagcgccagg cagtgcctag aacgaggtcc
     9361 agtcatcgct gcccgtcgcg gcaggagccg cggcggcagc cgggaccgtg tgggcggcat
     9421 tgaacgccgg cctgacgaca ttggtcgcac ggtgcggact gcgccgctcg ggggtctggt
     9481 acgccttcgg gggcggtgcc gcgacctggc gggctgtcgg cgcgggatgg acatcgccat
     9541 ggcgcagtct gaacagcgcc accgccgcga ccagctgctg cgcctgcgtc ttcaggctct
     9601 cggccgccgc agcgctttcc tcgaccaggg cggcgttctg ctgggtggcc tgatccatct
     9661 gcgtgacggc ctcgccgacc tgggccatgc cggtcgactg ctcggcgctg gccgcactga
     9721 tctcgcccat gatgtcggtg acgcgccgga tcgacgtcac gatctcgctc atcctggagc
     9781 cggcctcgtc gaccagttgc gtacccgctt cgacgcgttc gacgctggtg gtgatgagat
     9841 ccttgatctc ccgggcggcc gcagcactgc gctgcgcgag gttgcgcacc tcgctggcca
     9901 ccacggcgaa gccgcgaccc tgctcgcccg cacgcgcggc ttcgaccgcg gcgttcagcg
     9961 cgaggatgtt ggtctggaac gcgatcccgt cgatcacgcc gatgatgtcc gcgatgcgac
    10021 gagagctgtc gttgatgccc ttcatcgtgt cgacgacccg ctccacgacc tgcccgcctt
    10081 ccacggcaac cgacgacgca ctgatggcga gctggttggc ctgccgcgcg ttgtcggcgt
    10141 tctgcgaaac ggtggcggtc agctgctcca tcgacgctgc ggtttgttcc agcgcactgg
    10201 cctgctcctc ggtgcggctc gacaggtcgt tgttgccctg ggagatctgg gcgctggcgg
    10261 tggcgacgcc ttcggcgttg cggcgcactt caccgaccgt ccgctgcagg ctgtcgcgca
    10321 tgacattcag cgaagacatc aggctgtccg tgtcaccggg acgcagatcg atgcgaaccg
    10381 tcaggtcgcc atcggccacg gcctgggcca tcgccttggc ctcggccggc tcggcgccaa
    10441 gctggcgggt caggctgcgc accatccacg aactgaacgc cgcggcgaac agcagccctg
    10501 cagccatcgc ggcgatggcg aaggctcgta ccgtggcgta cttcgcctcg gcggaagcgt
    10561 attcctcgcg cgccacgtcg atctgcagct tcagcaaggc ctgcatgttc ttgtccacgg
    10621 gctcgctcag cgggctcagg acatcgtcga ccatctgggt tgcatccttc agccggccgg
    10681 ccttgagcgc ggccatcgtc ggctgcacac ccccgctctg gtagcgcgcc cgcgcgtcac
    10741 caaaggactg ggccagccgt gtctcctcga cggtcagcgg cctcgctgtg tactccttcc
    10801 acaacgcgtc gatcgcgacc gagttctcct gcaactcgct ctgcgccttg gcaatctctt
    10861 ccgcgcgcgg atcgccgatc gcgttgatca gcatggcttc ggtctcgagc tggcggcgct
    10921 ggatttcagc cagcgcgccc agcggtgcga cccggccttc gttgacgatc ttcagggact
    10981 ggttggcatc gctgatgccc cacaggccga tcgcgccgat gacgatcagc aacagcgaca
    11041 aggtacccgt cagcagcagc aggcgggtgg agactttgag ttggttcatg agacgaggag
    11101 gaagtgcagt cggagcctgg ccgcgacggc aaccactgcc gcaggccttc gtgggagccc
    11161 cgtcgatccg atcgacggtc ccccgttgct tcatcggcac gcccgctccg gtcttgagac
    11221 gggtctgacc gggccgccgg acgccccggt cctgccggaa caggtctcca gcggtgcttg
    11281 tcagccgccc ggaaccacct cttccgctcg agcggcggat atccagtgct cgatcaaggc
    11341 gtgcaactgg ctgatcatga atggcttggc gaggtgagca tccatgccgg cagccaggca
    11401 gcgttcgatg tcgccgttga gggcgttggc ggtcagggcg atgaccggca ggcgtggccc
    11461 accggacaga cgctcgcgct cgcgcagccg gtgcgtggcc tcgtagccat ccatgaccgg
    11521 catctggcaa tccatcagca ccagcgccac cttcgtttgc gccagcacgg ccagggcttc
    11581 ctcgccgtcc tctgccacca ccacctcgag ccccatggac tccagcatcg cggtaccgac
    11641 catgcgattg acgctgttgt cctcgaccag aaggacacgg ccactgaggc cttccggacg
    11701 ccgctcgtcg cgggactccg gcgcaacggc attcaccgga gccggtgccg gtgcggcagc
    11761 agccgggccg ggtgctcgct gcaacggcag cgcaaagctg aacacggtgc cctggcccgg
    11821 caggctgtcg accaccagtt caccacccat ggccctgacc aggtcgcgac tgatcgcgag
    11881 gcccaggccc gttccaccct gcgccggcct ggccgggtcc tgcgcctgga cgaagggctc
    11941 gaagatcctg tcgtgctgag tcttgtcgat gccgatgccg gtgtcggaga tctcgaaggc
    12001 aacctgttcg ctgacgcccg aaccgatgca gtgggtgccg accacgatgc acccgcgctg
    12061 agtgaacttg atcgcattgc cgaccaggtt cagcagcacc tggcgcagac gcagcgaatc
    12121 gccatagcgc cagccgtcga gcgccggatc ggaatcgagg cgcaactcga tcgacttgcg
    12181 ctgagccgat gcggaaaaca gcgtggtgac cgaccgggcc acggcgttca cgtcgatcgg
    12241 ctcgcgggcc agctgcaggc cgcccgcgcc gatcttggcg aagtcgagca cctcgttgag
    12301 gatgccgagc agcgcctcgc ccgaggtggc cgccacgtcg atgtactgcg cctgacgcgg
    12361 gttcagtgcg gtgtgcgaaa gcaggtcgag cgcaccgagc atgccattca tcggcgtgcg
    12421 gatctcgtgg ctcatgttgg acaggaactg ctccttggcc cgggcagccg ccagggcctc
    12481 gtcacgcgcc tgctcgagct cccgggtgcg ctcggccaca cgatcctcga gcccggcatt
    12541 gacgaccgcg agctgctgct gcacccgcgc ctgttcacgc tgaccctgct cgatgcggcc
    12601 catcatgtcg cccagcgtga gcgccagcgt cccgacgtcg tcgccgcgat ccgccaccgc
    12661 gctcaggcgc aactccaggt cggacgacat gaccggcgtg gcggcgaact ccgtcgccgc
    12721 ctgccgaagc tgtgccaggc gccgtgcctg cgaacgcacc caccggatca gcagccacca
    12781 gcccaggacc aggtaggcgc ccgtgcaggt gccggcgatc agcagcagtt ccagccccga
    12841 gatcggggtc acgaactggc gatcgatgcg caacatgggt gcgccgggcc ctgcatccca
    12901 gcgcagcgac aggttcgttc gctggtctgc cgacactgcg gacgcgcgag ggccgctcat
    12961 cagggcgccg gcatccgagg tcgccacggc ctggccttcg tggagcaggt cgagctgcgt
    13021 gttgggatag gcgagctgcg ccagcagcgc gttatcgagc ggcacgtaca acagcaactg
    13081 ggccggtcgc ccggcctggc gcaccggccc ggtcaccgtt cgatagaccg tgcgatcgcg
    13141 gtcgctgtag acccatccta gacccgccgc atcgctgtcg gctggcagcg tcagcaggtc
    13201 ctgcgagcgg gtgccgtatc ggaacagcac ctgacccgcg tcgccgacga tggctgcatg
    13261 cgtgaattcg ccctggtcca gcaaggtggt catcagcgcg cgcaggcgag cctcggccac
    13321 cacctccggc aggcgttcga catccacctg ccagagctcg gtctgctggc gcacgctgaa
    13381 agccgactgg cgccagccct gatccagcgc ccggatgcgg gcctcgaagt gactgcccgt
    13441 gtcgcgccgc tgatgctcca gcccgtgttc gatcacgctg cgcgtgaaca gcgcgaaggc
    13501 cgcggccacc gccacgaaca ccaggaccag catgccgaac agtcgccgag tcaacgacat
    13561 gccggcgtcg gagcgcgccg cttcggtggc cgctgcctga ttcatggttt tcggcccgcc
    13621 cagcggtcca cgatcatcga cggcagccga tacgcgacgg cctccggggc ctgcccggca
    13681 ctttcgccga agaacgtctc ggtctgcgcg atctgcgcct gcgaaaaacg gccgtcggga
    13741 ctgaactgct gcgggtactg ccgcagcatc tcggcgatcg ccagcgcatc ggcgccggtc
    13801 aatccgagcg catcgacgac ctgctgtggc gtgtggtcgc gcctccaggc cagcgtgcgc
    13861 tgaaccacac gcaccatctt ctgcgccagc tcaggccgtc cctcgacgag gcccaccgag
    13921 ctgatcaagg cgccgcgcag gaagccgacc cccggcaagg atcgtggatc ctcgggatgg
    13981 ccgatgcgaa agagcgggaa cgccagccct tcctgggcca gacgcaacgc gaacggctct
    14041 tccgacacga tggcatcggc cacctgggcc cgcattgcac tggcctgcgt gtcccaggac
    14101 tgcccggcgg cgacgaaccg gaccgactcc ggcgacaccc catgctggcg cagcacgagc
    14161 acgagaaact gctgccccgt ggtgctcgac gtgagcgagt tgctgtggat gccgagcacg
    14221 cggcctcgca ggtcctcgac gcgacgcacc ttgtccttca ggtcgctgcg caccatcacc
    14281 gaaagcagca ccctgtcctc gatcgccgcc agcgccacca ggcgacgcga ttcgaggttc
    14341 tccttcatcg ccgcaggcac tccgaagaca ccgaactgcg cgttgccgga cgccatctca
    14401 cggatagcga ccccgccgcc ggtcacgaac ttcaggcgca gcgccagccc ttcggcccgg
    14461 tctgcaccga tgcgcacggc aagctcggga atcagcgaca ccgcgctgga gggccccgga
    14521 gtggccagaa ccacctcggt ggcggcagcg gcctgcgcgc gaacatcggc ggacgcccag
    14581 agcgctgcgg cagccagggc tgcgcgcttc aggagcaggc gacgcgggag gaatggacag
    14641 gtgcgcacaa gactctcgct gtaacgaacc gtgcatcgat ggtagccctg caaagcccgg
    14701 gcgcacggag cgaacagggg gcgaataccc caccctccct tatgcagcag gcaaccaagc
    14761 ttagaagcac tgtgtcgttg cctgccgagc atcgcatcgt gagaatcgga cgctcggcat
    14821 atgccgaaaa caactatcgc ccaccgggcg acgacccatg aatttccagc agcttcgagc
    14881 cgtccgtgaa gccacccgca cgggattcaa cctgaccgaa gtcgcgggcg ttctgcacac
    14941 ctcgcaaccc ggcatcagcc gacagatccg cgaactggaa gacgaactcg gcatcgacat
    15001 cttcgtgcgc gcaggcaagc gcctgacgcg gctcacgcct ccaggagaag cggtgctgcc
    15061 gatcgtcgaa cgcatgctgc tggaagccga caacctgaaa cgtgtcggcg acgacttcaa
    15121 cgcgcaggac accggccgcc tgtcgatcgc tgccacccac tcccaggccc gctacgcgtt
    15181 gccgaccgtg gtgcgcgact tccgccagat ctaccccaag gtcacgctgc tgcttcatca
    15241 gggctcgccg aagcaggtgg ccgagatgct gctgtccggc gaggccgaca tcggcgtggc
    15301 gaccgaagca ctggcgcaat acgaatcgct ggtcgccctg ccctgctacc gctggacgca
    15361 cagcatcgtc gtgccacccg gccacgaact gctcgacatc gacggcccga tcaccctcga
    15421 acacctggcg cgccatccga tcatcactta cgagaccggc tacacgggcc gcgcccacat
    15481 cgacgacgca ttcgcgaaag cggggctgac gcccgacatc gtgctcagcg ccatggatgc
    15541 cgacgtcatc aagacctacg tcgagctcgg catgggcgtg ggcatcgtcg cttcgatcgc
    15601 cttcgacgcc gagcgcgacc gcacgctgcg cgcgctcgac gcgcggcacc tgttcgagat
    15661 caacctgacg cggctggccg tccggcgcgg cacctggctt cgcggctatg tgtacgcgtt
    15721 catcgaggcg tttgccccga cgttgtcgag accagtggtc gaacgcgcgc tcgccggtga
    15781 tgtccatgca gagtgacgcg ggcagcccgc cgcccggtca cccgaccagc accgggtcgg
    15841 tggttcgacg tcccgagtaa tccgtccagc agcgagcctc gtagtcgtag agcttgcagc
    15901 ggcgcgccgt gtcggcatac cagcgccacg agaaaggctg gatcacgatg cgtcccggca
    15961 gcatggtttc gagcagcttc tgcgctcgct tcagccggta cagcggcacg ctcatgtcca
    16021 catgatgtgc ggtgtgctcc atgatgtgat gcagcacgct gcccatcggc gagcggaaag
    16081 tcaggtgaac cgtcgtcgac acgaacggcg aggtgctggc ccagctggcc ttgtcggcgt
    16141 accaggccac gcccgagtgc gtgtggtgca catagacgac gaagccgatc atgccgttcc
    16201 agaagacgaa aggtatgacg aagcccaccg tcaccagcca ccacggtgac tggcccgtcg
    16261 cggacgccgc gagcaccaga ccgccgatcc agaccgctgc accgatggac acgccgatgc
    16321 agtcccagag gaaggccttg cgacgggtcg gcatgacttc cttgctcggg aacatcatgc
    16381 ggccccacca gatctcgacc aggtaataca gccacggcgc ccagccgctg cggtagacgc
    16441 gctccagcct ctgcctccac gccggcaagg cctggaattc ctgcagcgtt cgcggttccc
    16501 agacgaagtc gaagcccttc aggttggtgt agccgtggtg caccaggttg tgcccgatgt
    16561 cccagaggct gtagggtgtc atggacggca ggaagaccag tcggcctacc cagcggttca
    16621 gcgtgcgatg ccgggtcagg ctctggtggc aggcgtcatg gccgaggatg aagaggcgcc
    16681 cgatcacgaa gccggtggcg aggccggcca gcacctgcaa tgcggggtga ctggcctgca
    16741 cggtgatcac gagaccggcg gcaaacagca ccagatcgaa gagaaccagc cacagcgcgt
    16801 agacggtgct gcgttcgcag atcggcgtca gccaggctcg gatcagtttg cgatggggca
    16861 tcggggcctg tgcggccaac ggctcggtgg ccggatagag aagcgtcgtc atgggctggg
    16921 attgccggtc aggaaaatgc ccgagcctag tgaaaaaccc gcgcgcacag cttgaccccg
    16981 atcaaggcgc gaagcacctg ttgccatcgt tgatccgatt gccgcatcgc agtgaccccc
    17041 gtatatttcg accagaaagt tcagattaat cgatttcgga gggtgggttg cgacactggg
    17101 tcaaattgat cgccggccag atcggcctgc acgagccact gcctttcggc gtccacgacg
    17161 aaaacgggaa gctgcttctt gcccggggcc aggtgatcga caacgaagca cagatggcgg
    17221 cgctgctttc gcgcggcgta ttcgccgacg aaggcgagat cgaggcggcc cgcaacgggg
    17281 ccccacccga aaaggccgcc aagcgcctga cgatgttcga cctgtgggaa caggcgatag
    17341 ggcgtctcga tcgcctgatc aagagtgtgg gtcaggagcc tgatttcgcc ggtcgctgcg
    17401 acgagttcgc cggccagttg atgggcctgg tcgaacgcga tccggacatc ggcatctacc
    17461 tgtcggtgcg gcaggacgaa agcaggttgc atctgtacgg gctgacccat gcgctgcact
    17521 gcgccctcgt gtgccagttg atggccttgc gcatcggctg gccggtcgat cgcacgcgac
    17581 gactggtgaa ggccgccctg acgatgaacc tgtcgatcat ggagtttcaa gggcgctacg
    17641 ccgtccacgg ccgtttcgac gacgaacaga aggcgcagct gcgcctgcac ccggcgcagg
    17701 cggtcgagcg cctgagggcg gcaggcatcg acgacgagat ctggctgcag gctgtgatgg
    17761 aacaccacga gcgaccgggc ggcggcggat acccgaatga tctggtcgag ctgtcggaag
    17821 aggccggcat cctgcgcatg gccgacatct tcatggcgaa gatcagcccg cgcgcgaccc
    17881 gcgcgccgct gtcgatccag gaggctgcgc ggcagatgta ccaggaggcc cacggcagtg
    17941 cagcggcggc ggccatcatc aaggagtacg gcatctaccc gccgggcaac ctggtgcacc
    18001 tggcctcggg cgagctcgcg atcgtgatcc ggcgcggcgc cacggcacac accccggtgg
    18061 cagcggccat caccgacaag tccggggtgc ccacgatcag cacgacgcgc cgcgacaccg
    18121 cccagcccgc gtttgccatc aaggccctcg aattcgaccg ttcgctggcg ctgcgcgtgc
    18181 cgccggagcg gctttacggg ctggccgaat agcggcgcag cgggtcagat cgcagcagct
    18241 ggatcgggtt gctgttcctc ggcgaagcgc gtgatgcggc gtggcaccag atgcaccgtg
    18301 gtgccgggct cgagcgtcat gcgctcgcga agttgcctga attcggcacg cggcagttcg
    18361 acatcgatga agctgccgtc atcgagccgc ctgaactcca gccgcgtgtt gggaccgacc
    18421 gtcagcgcct gcgacagcgt gaccgccacc gcaccctcct gggcagtcgc cacgatctgc
    18481 aattcgtgcg ggcgcacgta gctcacatcg gccgcgtccc gcaccgcgcc ctgcgcgtgg
    18541 tcgagtcgcc cgtggaacag gttcacgtca cccaggaact tcagcacgaa cggcgtcgcc
    18601 gggtggtcgt agacctcgtc gggagaaccc acctgctcga tgcgtccctc gttcatcacg
    18661 acgatgcggt ccgcaacctc catcgcctcc tcctggtcgt gggtgacgaa cacgctggtg
    18721 acatgcacct cgtcgtgcag cctgcgcagc cagcgacgca actccttgcg caccttggca
    18781 tcgagcgcgc caaacggctc gtcgagcagc agcaccttgg gctcgaccgc caatgcgcgg
    18841 gccagcgcga tgcgctggcg ctggccgccc gaaagctggt gcgggtagcg gtcggcgatc
    18901 cagtcgagct gcaccagctt cagcagatcg gtgaccttcg cgcggatctg cgcctcgctc
    18961 ggccgcgtgg ccttcggccg cacgcgcagc ccgaacgcga cgttctcgaa gatcgtcatg
    19021 tgggcaaaca gcgcgtagtg ctggaacacg aaaccgacct ggcgctcccg gacgtcggtg
    19081 taggtagtgt cctcgccatg gaacagcacc gtgcccgaat cgggcacttc cagacccgcg
    19141 atgatgcgca gcagcgaggt cttgccggat cccgacggcc cgagcagcgc gaccagctca
    19201 cccgacggaa tgtcgaggtt caggttgtcg cagaccaccg tcttgccgaa gcgcttgttc
    19261 aggttgcgga cttcaatgct catgtcgatg actccatcgg gattcgcttc tcattcattc
    19321 cagcaaatga gagtgcagtg agttttcctt tgtctgcgcc gcagagcccc ggccgagccg
    19381 gcctccgcgg gctgaatcct cgctcggccc tgaacgggcc gagccttggc ttcttgatgc
    19441 ccgctgcgac cggcccggcc gaggcgctgc ggtagtggtg cattcgaccc aacggcagag
    19501 atgcgggcag cggattgggg ttggggttgg gttgaattcg agtcagccgc aaacacgcca
    19561 gcacgggtgg cctcgggccg cggccagcgg gcatgaggaa gcccaggttg ggcccgttca
    19621 ggggccaacg ggtattcagc ccgctggccg cggcccgaga ccacccgtgc cgctcgaact
    19681 tcacggcaga agaatcaagc acgctcatcc accgctcgct tctgcgcctt gacccgctgc
    19741 tcgaccacgt acttcagcac cagcgtcacc agggccagca aggccagcaa cgaagccacg
    19801 gcaaaggagg ccgcgaactg gtattcgttg tagaggatct cgatgtgcag cggcatcgtg
    19861 ttcgtctggc ctcggatgtg ccccgacacc accgacaccg cgccgaactc gcccatcgcg
    19921 cgggcattgc acaggatcac gccgtaaagc agcgcccact tgatgttggg catcgtcacg
    19981 cgccagaaga tctgccagcc gctggcaccg agcacgcagg cggcttcttc ctgctcggtg
    20041 ccctgcgcct gcatcagggg gatcaactcg cgcgcgacga acgggaacgt gacgaacacc
    20101 gtggccagca cgatgcctgg cacggcgaag atcaccttca tgtcgtgatc acgcagccac
    20161 tcgccgaacc agccctgcag gccgaagatc agcacgtaga tcaggcccga gatcaccggg
    20221 ctcaccgaga acggcaggtc gatcagggtc agcagcacgt tcttgccgcg gaactcgaac
    20281 ttcgcgatgc accaggccgc agcgacaccg aacaccaggt tcatcggcac gctgatggcc
    20341 gccgcgatca gcgtcagctt gatcgccgac agcgcatcgg gctcggtgat cgaggccagg
    20401 tacacgtcga cgcccttctt gaaggcctcg acgaacacga tggtcagcgg gatgaacagg
    20461 aagaacgtca ggaaagccat cgcgacaccg atcagcgtcc acttgaccca ggtgggttcg
    20521 gtcgtcgccg cacgcacgct cggcggcgca ctggcgcggc cggcatgcat cccggaaccg
    20581 ggcttcaggg acagggcaga agcatcgtgg ctcatcggtt catcgtccct gacgcgcccg
    20641 ggtccaggcc tgcagcgcat tgattgcgag cagcaggctg aaagcggcga ccagcatcac
    20701 gaccgcgatc gcggtcgcac cctggtagtc gtactgctcg agcttggtga tgatcagcag
    20761 cggcgtgatc tcgctgacca gcggcatgtt gccggcgatg aagatcaccg agccgtattc
    20821 gccgagcgcc cgcgcgaagg ccagcgcgaa gccggtgatc aacgcaggcg tcaggatcgg
    20881 gaagatcacc ttcgtgaagg tctgccagcg gttggcgccc agcgtggcgg ccgcctcttc
    20941 cacttccttg tgcaggtctt ccagcaccgg ctgcagcgtg cgcaccacga acggcaagcc
    21001 gatgaaggtc agtgcgacga acacgccgag tggcgtgtag ctgaccttga aaggcagcca
    21061 ctgaccgatc cagccgttct gcgcgtacag cgccgtcagc gcgatgccgg ccaccgcggt
    21121 cggcagcgca aacggcaggt cgaccagcgc atcgacgacg cgcttgaacg ggaactcgta
    21181 gcgcaccagc acccacgcga tgatcagtcc gaagaacgcg ttcagcaacg cgccggccag
    21241 cgatgcgccg aaggtcagcc ggtacgaggc catgacccgc ggcgacgtga ccgcgtccca
    21301 gaaagccggc caggtcatcg tggcggtctt caggaaggcc gcgctgagcg ggatcagcac
    21361 gatgaagctc aggtacagca gcgtgaagcc gagcgccagg tcgaagcccg gcagcacgct
    21421 gtgccgccgc agcagggtgc gggaaaggaa cacgtcaggc cccggttact tcttcgaacg
    21481 cagcacgatc tggtcgtaga agccgccgtc gtcgaagtgc tccttctgcg ccttcttcca
    21541 gccgccgaac acctcgtcga cggtgaacgc gttgaccttc gggaaatcct tcgcgtactt
    21601 ggccagcacc ttggcgctgc gtggacggat gtgctggcga gcagcaatgt cctgcccttc
    21661 gtcggaccac aggtacttca gataggcctc ggcctgcttg cgggtgccgc gcttgtcgac
    21721 caccttgtcg accaccgcca ccgggttctc cgccagcacc gtccacgtcg ggtagatcac
    21781 ctcgtagtca ccgccgaact cgcgcttgat cagcggcacc tcgctctcga aggtcaccag
    21841 cgcatcgccc aggttgcgct gcgcgaacgt cgtggtggca ccgcgcccgc cgccgtcgag
    21901 caccggcacg ttggcgaaga tgcgctcgac cagcgccttg gcggcggcag gtgccacccc
    21961 gttcttcagg cccgcacccc aggccgcgag ataggtgtag cggccgttgc ccgaggtctt
    22021 cgggttcggg atgatcaccg agatgcccgg ccgcccgagg tcgctccagt cgcggatgtt
    22081 cttcggattg cccttgcgga cgaggatcac cgacagcgag gtcgtcggag cactgttgtc
    22141 gggcaggcgc ttcgcccagt cggcggggat cagggcaccc cggtcggcca ggatgtcgat
    22201 gtcgttggcc tggttcatcg tgatcacatc ggcttcgagt ccctcgacca cgctgcgtgc
    22261 ctgcttgctg ctgccgccgt gagactggtt cagcgtcagt tcctcgccgg tggtcttctt
    22321 ccagtgcacg gcgaaggccg cgttgtattc cttgtagaac tcgcgcgaga cgtcgtagct
    22381 gacattcagc agggtcttgc cagccccttg cgcggaggcg gtgaaggagg tcagcgcagc
    22441 ggtgctggcc aacgtgacga gcaccgcgcg gcgggaaact ggagtcatgg tgaggagtgg
    22501 ccgaacatcg ccacgaagtt tcaaaacggc catcctaggc agcacccatc gaatggacaa
    22561 cgatgcattc cgtcgttgct tatccgaaaa acaagtaagt ccggctgctc ctcatccggt
    22621 ggcagccggc tgttcagccc cgcagggtgg ccgtgatcga gctcaccccg gggctgcaga
    22681 aatcacttca cggtgtagat cttgtcgaac acaccgccgt cggcgaaatg ggtcttctga
    22741 gccttcgccc agccaccgaa gacggcatcg atggtgaaca ggttgatctt cgggaagttc
    22801 ttctcgtact tcgtggccac cgccgggtcg atcggccggt agaagttgcg cgccgcaatc
    22861 tcctgccctt ccgccgagta caggtactcg aggtaggcct gagccacgtc gcgggtgccc
    22921 ctcttgtcca cgaccttgtc gacgacgctg accggcggct cggccaggat gctgaccggc
    22981 ggatagacga tgtcgaacct gtcagcgccg aactccttca acgccaggtg agcctcgttc
    23041 tcccaggcca gcagcacgtc gccgatgccg cgttcggcga aggtcacggt cgaaccccgc
    23101 gcgcccgaat cgagcaccgg cacgttcttg aacaggctgg ccacgaactg ctgcgccttg
    23161 gcgtcggagc ctcccggctg ctgcagcgcg aagccgtagg ccgccaggta gccccagcgc
    23221 gcaccgcccg aggtcttcgg gttggcggtg atcacgccca caccgggctt gatcaggtca
    23281 tcccagttct tgatgccctt cggattgccc ttgcgcacca ggaagatgta ggtcgaggtg
    23341 tacggcgagc tgttgtgctt cagacgcttc tgccagtcgg cgggcagcac cttggccttc
    23401 gcggcgatct cgtcgatgtc gtaggccagc gcgagcgtca ccacatcgga gtcgatgccg
    23461 tcgatgaccg atcgcgcctg cttgcccgag ccgccgtgag actgcctgac ggtgacgacg
    23521 tcgccggtcc tggccttcca gtacctggcg aacgcggcgt tgtactcgac gtaaagctcg
    23581 cgcgtcgggt cgtacgacac gttgagcagt gaaacgtcct tggcggcagc gccgaaggct
    23641 gctgcactga cggcaagcac gagcagtgcg gaacgggtga agcttgatgc ggacatgagt
    23701 gcgaatcctg gatgagagac cggcgctgag cgaagacatt ccgacagctg acaggtcatc
    23761 ctaggcagcg acacgcgcgc agaaaacgaa gcgttccgcg cttgcatatt cggaattcga
    23821 cgaaccgaca ccgtgcacgg ctcggtcgtc gcgagcgtgt ccatccgctc atcgtcgagc
    23881 gcgtcgccgg ggggtgacat gctccgcaga ccccacacgc gatccgagag aagcaaacaa
    23941 aaagggcccg ccaaggcggg ccctcttcaa gccgaagctc aagacgtcag aacgattccc
    24001 agctgtcgtc ggtgccggtc ttcagcggct gctggcgttg cgcggcagaa gactgtgaag
    24061 cggtggccgc ggccttgcga ggcgtgaacg tcggacgaac gacgttgcgg gcacggttgg
    24121 gtccgcggcg ctcctcgcca ccccagccgg cggtctcggc ctgcgctgcg gcaaccatcg
    24181 acccgccatt cgattcggtg cggaaggccg agaccgcctg caccagttgc tgcgcctgca
    24241 cccgcaggct ctcggcagcc gccgaacctt cctcgaccag cgcggcgttc tgctgcgtga
    24301 cctgatccat ctgggccacc gcttcgccga cctgcgccac accggcgctc tgctcggcac
    24361 tggcggcact gatctcgctg accaggtcgg tcacgcggcg gatcgagacc accacttcgt
    24421 tgatggtggc acccgcctga tcgacctgcg ccgagcccgc ctcgacgcgt ccgacgctgt
    24481 cgttgatcag cgtcttgact tcacgtgccg cttcggcgct gcgctgtgca aggttgcgca
    24541 cctcgctggc caccaccgcg aagccgcggc cctgctcacc agcacgggca gcttcgacgg
    24601 cagcattcag cgccaggatg ttggtctgga aagcgatgcc atcgatcacg ctgacgatgt
    24661 ccgagatgcg cttcgagctc tcgttgatgc ccttcatcgt ctcgacgacg cggcccacgg
    24721 cctcgccacc tcgaacagcc acttcggagg cgctcgatgc cagctggctg gcctggcggg
    24781 cgttgtcggc gttctggcgc acggtgctgg tcagctcttc catcgacgcg gccgtttctt
    24841 ccagcgcgct ggcctgctct tcggtgcgct ggctcaggtc gaggttgccc tgtgcgattt
    24901 ccgacgatgc caccgccacg ctggaggcgt tgcggcgcac gtcgccgacc gtcttctgca
    24961 ggctgtcgcg catggcgttc agcgacgcca tcaggctgtc ggtgtcgccg gggcgcagat
    25021 cgatctgcac cgtcaggtca ccctcggcca cggcctgggc cagggccttc gcatcggccg
    25081 gctcggcacc gagctggcgg gtcaggctgc gcaccatcca cgtgccgaag accgctgcga
    25141 agagcaggcc ggccacgatc gaaccgagcg ccaccgcacg gatcgtcgcg tagctcgaca
    25201 aggccgccgc gtactcctct gcggcgactt cgttctgcag cttcagcaag gcttcgagat
    25261 tctcttcaac cggcttgtag aggggattca gcaattcgtc gaccatcttg ccggcatcct
    25321 tgacccggcc ttccttgagc gcgaccagcg tgggctgcag gccttcgctt cgatagcggg
    25381 ctcgtgcgtc gccaaaggcc tggaccagcc gagcttcctc ggtggtcaag aacgtggccg
    25441 tgtaggcctt ccagagggca tcgatcgcgg cggcattgtc cggcagcgtc gttgtcgccg
    25501 tggcgatttc ctcgggacgc gggtcgccga tcgcgttcag caacgcagcc tggctcttga
    25561 gcatgcggcg ctcgatctcg gccagctggc ccagcggcac gacgcggtcg gcgttgacgg
    25621 tcttcaggga ctggttggca tcgttgatgc cccacaggcc gatcgcgccg atgacgacca
    25681 gcagcagaga cagggtgccg atcagcagca gcaggcgggt ggagactttg agttggttca
    25741 tggcagtgat tccgtggtcg ttcagttcag tgcagggcgg cgacggcaac acctgccgcg
    25801 gagccgacga gggaggcgat gtcgaccagc acgagcatct ggtcatcgat ggtggcgatg
    25861 ccgcgcaggt gcccgctcga cgagaagccc ggcatcgacg gcaccggccg caggtgttcg
    25921 gcggcgagat cgagcacgtc gctgacgctg tcgaccaccg cacccagacg cttgccgtcg
    25981 cgctcgacca cgatcaccga cttctgcgcg tcgtactcac acgccaccgc gccgaggcgc
    26041 agccgcaggt cgacgacggg caccatctcg ccgcgcagat tcatcacgcc gagttggtcg
    26101 cacggcgaat gcgcgagctt catcggcgcg ctgtacgagc ggatctcgca cacctgcagg
    26161 atgtcgaccg cgtaggactg cgatccgatc ttgaaggcca ggacgcgctg accttcgggt
    26221 gaatctccgg gttcaggccg gactacttct gttcggttgg ggctcgtcag ggtctgcatc
    26281 gtggtttcct ttgggtgagg tcgtcacgct gaaacaccgc gagctcgacc agaaaccaaa
    26341 cgatacaagt gatttacgca tttgtatcat ttaagaggcg caaattgcga gatttttatc
    26401 acttttaact ggatctgatg accagtccgg ccagcagccg cggtcgcagc cgcccctcga
    26461 caggggatca accgcccgtg gaggcgtcgc tcgacaggcg ctgcaagacc gcgcgcaggt
    26521 cggcccaggg cgctgccgcg gccgtccagt cgcccgcagc cgcggccgat tcgagccgtg
    26581 ctgcaccgtc ggcctccgcc tcgtggccga gcgtgcgcaa cacgctcttg aggctgtgcg
    26641 ccagcctgcg catgccctgc gcgttccgcg cgcccgccac gcgatcaccc tcttgcaggt
    26701 cgtctgcaaa ctgcgtcagg caggtcgccc tgaacgcatg gaacagcggc gcattgccgg
    26761 caaagtggcg atcgatcgcc gcttgctcgt gcacatccac aaccggtacc gctgcagttt
    26821 cgtcagccgc cggcactgca cgggccgcag gatcgtccgc gagatcgaga cccagtgcgt
    26881 cgagcacgca ccgctcgagc accagcatcg aagacggctt ggtcaggaag cgccacacgc
    26941 cgagcgccgc catcgtgcgc tccaactcgg gcgagatgcc ggcactgaac accaccagcc
    27001 gcgcaccggc gcgcagtgcg ggatcccgct ccagcgactg cagcaattcg aggccggtct
    27061 cccccggcat catcaggtcg gtgatcagca ggcgaaccgg cgactcgcgc agccgctcgc
    27121 gcgcctgggc cgcgccttcg caggtgatca gctcgatcgg catgccgtcg agggacatct
    27181 cgaccagccg gcggatcgat acgtcgtctt cgaccagcag gacgcgcgga agcgccacgg
    27241 cagagactca ggccgcaggg cacggggtcg ctgcagccgc ctgcagggcg gcgttcagca
    27301 aggccggcag ctcgaccacc agcttcggct tgtgcaccac cggcacgccc gtcagcgcga
    27361 aggcgtaagg ctcgagctcg gccccgtcga gcgaggtgat gacgatcaac tggctgccgg
    27421 cgaaggtgcg atgcgatcgc agcccggtga tcagcgtggc gccgtcgatg cccggcagca
    27481 ggatgtcgac cagaagcact tcgggctgca gctgccccgc catcgccaag ccggcgatgc
    27541 catcgtcggc cacgctcagc tgggcctgcg ggaactgctg ctgcatcagc agcttgatca
    27601 ggttctggta gtggcgcgag tcttcgatca gcagcacccg gggggcgccg cgccgttcgc
    27661 tggaggtcgg ggtcgtgttc ggggcagtct ccggacgggc gggcgcagca gcgtgcgcat
    27721 cgacggccgc cccgccagct tgtcgctggg ccagccagcg ctgcaccgag gcacgcgaga
    27781 tgcgccggtg cccgccagcg gtcttccacg cgacgagttc gccccggtcg accatcagtt
    27841 gcaccgaacg cacggccagg ccgagctgct tggcgacctc gatcgtggtg ctgacatcgt
    27901 cgggcgcggt gtcctgaccc gaaaagtctg tcgtgtgcgc tgtcatgtgc gtaattctgc
    27961 cgcttttatc gcgtgatggc gaaatcaagt tgcacgcgca atatcatttt gatcacaatt
    28021 gccacatcaa atatcaaagc ctgcacggac ccgcatgaaa aagatcctga tcgtcgaaga
    28081 ccacgccgac atccgccggc tgatccgcat gaccctcgag ttcgaggagt gcgagatcca
    28141 cgaagcgccg gatgccgcca cgggctggga catggcccgc gaactgcagc ccgacctggt
    28201 gctgctcgac gtgatgatgc cgggcgacct cgacggcctc ggcctgtgcc gcgcgatgca
    28261 gtgccattcg cggctgcgcg acgtgcccgt gatcatgctg tcggcgcgcg gcagcagcgc
    28321 cgaccgcgaa gccggcctgc aggctggcgc caccgcctac ctggtcaagc cgttcagccc
    28381 gatgcagttg ctgacgctgc tcggcagcac cgccaccgcg cactgaagga cacacgatga
    28441 acgcgatgac cgaagcggcg tctctcgccg gcagcaactg tttcgaggat gccgcctcgg
    28501 cgcagatgga gcgcctggtg gccgatttcg gccggatgta ccacgagcgc aacgaagcgc
    28561 tgcaggaagt ggcccgcgcc caccatgacg cgctgctgcg actgtcgcgc gccgcggagt
    28621 tccgtgacga cgacaccggg gtgcacatcg tgcgcatggg ctacctggcc tgggcactgg
    28681 ccctgcggct cggccagacc gagcagtacg cgatgctgct gcggcagacc gcgccgatgc
    28741 acgacatcgg caagatcggc atccccgacg cggtgctgaa gaagccaggc agcttcaacc
    28801 cggaagagcg cgcggtgatg aaccagcatc cgcagatcgg tgccgagatc ctcggccgct
    28861 cgcgcatccc gctgttcacc gccgcggcgg aagtggcgct gtcccaccac gagcgctggg
    28921 acggcagcgg ttatccgagc ggcatggccg gcgcgacgat tccgctgtcg ggccgcatcg
    28981 tcgcggtggt cgacttcttc gacgcactga cgatggaccg ctgctaccgc cccgccttcg
    29041 aggaccacgt cgcgctggag atgctgatcg agcagcgcgg acgcgccttc gatccgctcg
    29101 tggtcgacac gttcgtcgag cacgcgaccg acctgatcga actgcgcgat caggtcaacc
    29161 tgcagaagct cgatttcgag gacctgtgca gcggcggcga tccgttcgtc aggcgcgggg
    29221 actgaggcga tggttcgccg cctgaagcgc cgggccaagc ccaccgatgt cggtctggcg
    29281 atcctcaccg ggatcgtggt gaccattttc ctgtggtcgg ccctggtcgg gttcgcgctg
    29341 cacgaaaggc gtcaggccat tgctgaagcc cacaaccaca ccggcgtgca gtcgcgtgcc
    29401 ttttccgatg gaatccgtcg cagcatcgac agcgccatcc tggccaacac cgcaatcggg
    29461 aaccagctcg agctggggct gcctgccgac ggcgagtcaa cgcgctccgc gatcgcccag
    29521 acggcgacca gcctgccgtt cctgctcggc ctgggcatca tcgacgcgca gggactcgtg
    29581 atcggtggca ccgacctggc ggccatcaat cgggtgatcg atctcaaggc tctgggcacc
    29641 ctgccgtccg tggaccagga ccggctcggg gaggccgtgg cagccggcag cctgctggac
    29701 ctggcgcctg cgagctcgcc tcggcgcaca cagccgggcg acagccttct tccgctgttg
    29761 cgcacggtgc agctgcccag cgggcaacgc gttcatctga tcgcgatgct cgatggccag
    29821 gcattcgccg ctgtccagcg cgacatgatg tccgattcga gcctgctggc cacgctggct
    29881 tcgagcgacg gcagactgat cgcgtcgact gcggtcaccg cacgcgcggc gggttcgtcg
    29941 atcaaggacg tgccgctttt ccagggcttg cttcagaagc aaccgcaggg cgaatggacc
    30001 ggcgcaggcg tgcgcggcac cgaacagatc gcttcgttca gttcgattgc gggccatccg
    30061 ctggtcgcga tggtcgagac cagccagcac gacgcgatgg cagactggct gaagaaggag
    30121 cgcctgctga tcatcgccgg gctggtgctc actctcctga ccgcgggtgg cacctggatc
    30181 gtcatgcgca gcctgcgcgc gcgcatggcg gcgcgcctcg agatcgacgc cgcccatctg
    30241 cgtgcctcgc agcgcgagac ggaactgacc gtgacgctca agagcctgca ggaactgatc
    30301 ttccgcaccg atgccaacgg cgccatcacc ttcgtcaaca accactggat gcccgtgatg
    30361 ggcacgcccg tggagaccgc tcaaggcgcg cgaccctggg agctggtgct ggcccgggat
    30421 cgggacgcgg tgtgggatct gttcgcaacc cgacccggcc tcgaggcccg ccgggcgcag
    30481 gcctcgctgg tcattgccac gggcgtggtg cgcaccttcg acttctcggt gacgccgctg
    30541 cagcacgaag gcgtggtcac cggctttgcc ggcagcgcca tcgacgtgac cgaacgcgtg
    30601 gcggccgaac gccagctcga ggcccagctc gccttcacgc ggctggtgat ggagctcagc
    30661 ccgctgccga agtcggtcgt ctcgatgaag ggacgccacc tgatcgtcaa caaggcgtgg
    30721 gaggagttca ccgggcagca gagcggcgac atcgcctttg aaacacagga cgactcggaa
    30781 caggcgactc cccggccact gttcgaggcg cgagaccgga agcggctcgc accgggtgag
    30841 ccgctgcgct acgagacgac ggtcaagcac cgcgacggtt cgacgcgcga tgtcatggtc
    30901 aacaaggtga tgctgcacgg gcccgacggc cggccggccg gcatcctgtg cgtgttcatg
    30961 gacatctcgg agttccgcaa tgcggagcgc gccacccgcg aagcgcggga catcgcagag
    31021 gaagcgtcgc gtgcgaagtc cgagttcatc gccaacatca gccacgagct gcgcacgccg
    31081 ctgcagtcga tcatcgggtt ctccgaactg ggccagatgc gggcccgccc gaacgagcgg
    31141 atggcggcga tgttcgacga catccacggc gccggccagc gcatgctgac gctggtgaac
    31201 gacctgctcg acgtgtcgaa gatcgacagc acggtgggtg cgatccacct cgaaaccaat
    31261 gatctgcgcg gactggtccg cggcgtgatc cgcgagatcc atccgctgct cgctgcgcgc
    31321 cggctgcagc tggtgacgca cctgcccgac gagccgatgc tcgcgagagt cgatccgccg
    31381 cgcttccagc aggtcgtgcg caacgtgctc gccaacgcca tcaagttctc gcccgcgggc
    31441 agccgcatcg acctcgtcgg cgatcacacg ccctctggag agttgcgact ggtactgcgc
    31501 gaccaggggc ccggcattcc ggtcgctgaa ctggagacga tcttcgaggc cttcgtgcag
    31561 tcgagccaca ccaaggacgg ctccggcggt accgggctgg gcctggccat ctgccggaag
    31621 atcatcgacg cgcacggcgg gcgcatccac gcccagaacc ggcccgaagg cggcgccgcg
    31681 ttccacgtcg tcctgccggc tcgcccggca ccgacgatcg acgaaagcga gcccgaatcg
    31741 gaagcagcca ccacctgccg gctcgcggcc tgagcacgct gccggtcacc ggcgggcacg
    31801 atcggtgccc gggagacgca ggccggcagc cccgctggct cagggcccgg gaacgatcag
    31861 ggaattgacg gtatcgatca acgccaacgg gctgaagggc ttgaccagat aggcgttcgc
    31921 gcccacacgc tggccggcct cgcggtcgct gtcctgcgcc cgcgcggtca gcagcacgac
    31981 aggcacgtgc gccagcaccg gatcggcctt gaccgccgcg cacacctgca ggccgtcgag
    32041 gtcgccgggc atcatcacgt cgagcaggat cagatcgggc ttcaggttgc gggcggcttc
    32101 cagtccggcg gcaccgttgg cggcctcgtg aatcgcgtaa tgctcgaatt cgagcgccca
    32161 gcggatcagg cgacggatgt cgggctgatc ctcgatgatc aggatcgact tcatgcgtgc
    32221 tccaaggcgg cttcgaccgg ggccgattca ggtgtggtgg gcagccacag cgtcacggtg
    32281 gttccatgcc ccggctcgct ggcgatggcc atgcgtcccc ccagcaactc gatgatttcc
    32341 ttcacgatgc tgatccccag tcccgtgccc ggtatgttgc cagacgcatc ggcgcgatag
    32401 aagcgctcgc cgacgcgggc gatctgttcg ggcgtcagcc cgatgccgtg atcgcgcacc
    32461 tcgatgccga ccatcgacgg ctcgctgtcg ccgtgcacga gccgcacctc gaccgccccg
    32521 ccctgcggcg agtacttgta ggcgttggcg accacgttgc cgatggcctg gcgcatcttg
    32581 ttgcggtcga cccgaacgat gatcgacttc gacagcggtg ccagctgcgc cgggctgcga
    32641 tcgtccggcg gcttgaactc ctgcagcagc tccggcagca gcgtggccag atcgagcacc
    32701 tcgtaggtga aatccttgcc ccgacgggcc tcgatgcgcg ccaggtcgag caattcgttg
    32761 acgatggcga tcatcagttc gctttgccga tggatggtcg agatcacctc ggcctgctgc
    32821 tcgggcgaca gcttgcggcg cgacatcagt tcggaaaagc cgtagatgct ggccatcggg
    32881 gtgcgcagct cgtgcgcggc tgtcgacagg aactcgctct tcatctggtc gacttcggtc
    32941 tcgtgcgtca cgtcgcgcag cgacagcacc tgcgagatgg cctcggtcga gcccaggcac
    33001 agccccgctt ccagcaccct cggcttcggc cacgccagat cgatcagctc gcgtcgcggc
    33061 tgggcttcgc cgacggcatc gcgcaggtcg cgacgcatcg actcgaagcc tcgccaggtg
    33121 gcggtggccg tgacccgctc gcgcagcatc gcctcgatgg tgccctcttc cgcgcccagg
    33181 atctgctgct cggtcagccc ggtcaggcgc gagaaggcag ggctcatgta gttggcacgg
    33241 cgctgcgaat cgaacgacac gaaaccgtcc ggactgagcg cgaagatcgc atcgagctgt
    33301 tcaccgcgct gctgcagccg gctgacgagc tcgaagatca tcatcgagac ggcctcgatg
    33361 tcatcggccc cttcgacacc gaccgaggtg tcgcggatca gcccttcgag cacctggcgc
    33421 agcgagatca gcgccatcgc gcgcgactgc agttcggcgc gcaggcgctg gtcgagggcg
    33481 tgctgttcgc tcatgtcgcg acaggccagc acgtgcccgg cgatctcgcc atcgggagcc
    33541 aggatcaccg actgtgtcgt gtcgaccacg acttcgcggc cgggcgacgc agacagcgtg
    33601 gcgcgacggc cggtccacga gctcagcggg tccacatgac ggtagacggc cggcaggacc
    33661 tcggtcatcg actcacccag cagttcgccc agcggccgat ccacgagagc acacgctgcg
    33721 ggattcgcga acacgaccct gccctgtgcg tcgagcgtga acacgctgtc ggcaatcgca
    33781 ctgagcgtga ccgctgcctg cgtacgctgc gctcgcaggc tctgcaccgt gcggccgatc
    33841 acttcgaacg tggcgcggaa ctcggtcggc gcgtcctcgg gcatcagcat ctcgggcgtg
    33901 gcctcgcccg cctgcatggc gcgctcgaac tcctggatgc gaccgaggtt gccgagccag
    33961 cgcaccagcg gaaagcggat cagcagcaag ccgcccgcca ggctcaacag ggccagcacc
    34021 agcgccatgc gcgtctgctc ccacagccgc ccggcgatgc gctccggaga aaaggtcagc
    34081 cgcagcacgc cgtaatcgcg gccgccgacg gtcaccgtct ggttggtgtc gtacagccgc
    34141 gaggccaccc ggtcgaccag ccagcccggc gcggacacat ccggcgggtc gttgcggggc
    34201 gcgcgcacga tgccaccctt caggtcgatg aagctcgcca cggagaagga cgaatgcagc
    34261 agcgcacttt caagcgtgcg gcggatggtg tcgtagtcgc cgatcaccgc gctgtcagag
    34321 atggtcggca tgatgacgcc gctgagcgtg tcggcgcgca gctgcgcctc ctccagctcg
    34381 accgagaact ggtagctgta gaacaggccc agaccgccga gaacgaaggc cagcagcgtg
    34441 acggtgtaga gcgcgaaaac gcgcccgacc agcgagcgcg gcagccatcg caccggcagc
    34501 ttcatcgagc agcagcctca gcgcagtgaa gggggagcag ttcgaaagaa gtcacggtag
    34561 gtggcgtact cgctgccatc cgatgcgatg aaattggcgt cgaccgtcag gcccacctca
    34621 gccgaggcct gcgccaggat ttcgcggccc ttcgggtcgc gggacatgcc gatgaatgcg
    34681 ccggcgacgg ccttctggat cttctcgggc accttcgacg aggccatcag cgccagatcg
    34741 tgaacgggcg gcgaagacca cagcacgcgg taggtcttgc cctcgcgctt cgcatagccc
    34801 tcggcgagct gcgagttggc gccggacgcg gcgaccttgc cgctgaacag ctgagccagc
    34861 gcgccgtcca tgttgccgcc gaagacggcc ttggcatcga tcttccgggc catcagctgc
    34921 gccatcggga acttgtaggc caccagcgct tccggacccg gaaacaccac ttccttgccg
    34981 gcaagctgcg agagctcggt gatcggcgaa tcggtgggga cgatgatctg ggcgtgcatc
    35041 ggcggcgtgg cgcgccggcc gaaaaccttc cagccgagct gctcgcgctc ggggctgaac
    35101 aggtggttgc tgaacacgaa ctcgacctcc tgcgccagca cgtaggcggt cgtgtcggcc
    35161 gaggtccgtc cgatcttcag ctgcagcttg acgccgctct tctcggacac gtaggcaatg
    35221 atgggattcc agtacgccgc ggtcaggttg atgccgtact ggttcacggg cgagaaccgg
    35281 taggcgtctt cctgggcccg tgcgggcccg gccaggaaaa gcaggccggc catgcacgag
    35341 gcagccagcc gggcgaggaa atgtgtgtgc atgatctggt tcagccgaag ccaaagggtg
    35401 gatgttgacc gatcggtcgc ggggctccgc ccgaaagtat cgagaagacg cccagccgta
    35461 acaaaagtta tcggcccgtg tccccggaac ttgaggcccg gatcgacaga gccgcggccc
    35521 caggtcaggc cggcaacggg gcttcgatgg actgcagcaa cctgtcgatc ggctcgggcc
    35581 ggtgtatcag gtagccctgc gcgaagtcga cacccatttc gcgcagctgc tgcatcacct
    35641 cggcagtctc gacgaactcg gccacggtct tcacgcccat caccttggcg acgtcggtga
    35701 agcagcgcac cgcggccatg ctcagcggat cacccaccag atcgcggatg aactggccgt
    35761 cgatcttcag cacatcgacc ggcagccgct tgaggtagcc gaacgacgat gccccggcac
    35821 cgaaatcgtc cagggcgacc tggatgccga acgccttgac ctgttcgatg aacgccccgg
    35881 cgtccgccag gtgcgtgacg gccgcggtct cggtgatctc gaggcacagc ctctggcaga
    35941 cctcggcgcc ggcctgcgtc agcagatcga cggcccagcg gtggaacgcg cgatcgccca
    36001 ccgattgacc ggacaggttg acgctgagcg tgtcgatggc cgtcagatcg ggcagcgagg
    36061 tcatccagcc gatggcgtga gacagcaccc agcggtcgat gcgcgaggcg aggtggaagc
    36121 gttcggccgc gggcaggaag gctccgggtg ggatgagcga gccgtcgctg ccgaccatcc
    36181 gcagcagcac ctcggcgtgc acgccgcttc gggcctcgcc cagcgattcg atgcgctgcg
    36241 cgaacagaac gaagccgtca tggtcgaggg ccagctcgat gcggttggtc cactgcatct
    36301 cgccatggcg agcgtgcatc gccgcgtcgg aatcgaacca cacgtgaact cggttgcgac
    36361 cggcctcctt ggcggcgtag caggaggtgt ccgcggcctg caggatggcg gaaatggagc
    36421 tccaccgctg gtcgaccggc accagaccga tgctggctcc gatgcggaac cgccgtccat
    36481 cgtggacgaa gcggtaatcg tccatctggt tgcagatctt ctgcgcaacg cgctgcgcct
    36541 gctcggccgt gcagcgctcg agaatgatcg cgaactcgtc ccccccgagg cgtgccagcg
    36601 tgtcgctggc acggatgacg ttgccgaaca gtctggacac ctgctgcagc agctgatcgc
    36661 cgaccgcgtg gccgcaggcg tcgttgacca gcttgaactg atcgaggtcg atgaacatca
    36721 ccgcgttctc gccgccgtcg tccctggact tcttcagcac gctgccgaga cggctctcga
    36781 acgcggctcg gttgagcaga ccggtcaggt cgtcgtgcgt ggcgcgatag ctgatctcgc
    36841 cgaccatctg ccgctgtgcg ctgacgtcgc ggaagaccag cacgaccccc agcacctggc
    36901 cctggtcatt gcagatcggc gaggccgagt cctcgatggc cagctgttcc ccgtcgcgac
    36961 agatcagcag cgtcttgagc accgggctgg gtgcgtcggc cttcgtcagg caggtcgtca
    37021 ccgggctttc gacggcctcg ccgctttctt cgtcgatggt gcggtagacc cgcgacaggg
    37081 actgcccctg ggcctctgcg gtggtccagc cggtcagctt ctcggcgacc gggttcagcc
    37141 aggaaacgca cccctcgggg tccgtggtga tgacggcatc gccgatcgag cgcagcgtca
    37201 cgcgcagccg ctcgtgctgc tcggccagtt cggcggccag cctgcgctgc tcggtgacgt
    37261 cccagttggt gccgaccatg cgcaaggcgg tgccgtccgc gtcacgcgtc acacgggcgc
    37321 acgctcgcag gtgatgcacg ctgccgtcgg gccagacgat gcggaattcg gtgtcaaacg
    37381 gcgtgtcttc gtcgatcgac tgctggatgc cccggtcgac cgccggcatg tccgcgcggt
    37441 gcacccgctg acaccagatg tgccacggat cggccgtttc gtcctggaag ctgccgaaca
    37501 agcggaacat ccgcgcatcc cagaccagtg cccgggtgcg cagatcccag tcccagatgc
    37561 cgatgccgcc actgtcggtg gcgactgccg cccgctcgcg tgccgtggac agcgcatgtc
    37621 gctcggccac ctgctgggtg acatcctgga atgctcccac caggcggcgc ggcttgccgt
    37681 cggcgatctc cagcgagccc acgacgcgca cccagatcga gcgtccatcg cgtcggatca
    37741 gcggaagctc gagttcgaaa ggcgcgccgc cggacatcgc aacctccacc gcgacgcgca
    37801 tggcgtcgcg cgcctcgggg gcatagaagg cgatggcctc gtccagggtc ggctggtaac
    37861 ccggcgacac gccatggatg cggcacgtct cgtcggacca caggatgtcg cccgacggga
    37921 gatcgacttc ccagcccccg acgccggcca tcttgccggt ccgatcgaga aagcgctcgc
    37981 tcttgcgcag cgcctcttcc ttgagcttgc gggcggtgac atcgaggtgg gtgccgtaca
    38041 tccattccgg cttgccgtcg ggcgtccagg tgatcacccg gccccgcgcg agcacccaga
    38101 cccagtgccc gtcacggtgg cgccggcgcg tctcgcattc gtaatggtcg atctgcccgg
    38161 caaagtgcct ggccaggatc tcggtcgagc gcaccatgtc gtccggatgg cctcgggcca
    38221 tccacgactg gatcgaggtc ggttccagct cggccagcgt gtagccgagg atctgggccc
    38281 agcgctcgtt gaaatgcacc tcgccggtct gcacctgcca ttcccaggta ccggcgtgcg
    38341 tgccttccac cagattggtc aggcgctggc gctcggtgct caatgcacgt tcggcgatgc
    38401 gctgcagcgt gacgtcctcg atgacgccga agctgcgcac cggacgaccg tgcacatcgc
    38461 gctccaggat cgccgaaagg cgcacgtcga tgacgccacc gctgcgggtg accatctggt
    38521 aggcgatgtt gtcgacgcgg cccgactgga agaaggccgg catcacgacg ttcagggcat
    38581 gttcgcgcga ggcgctgctg aggaaggcag ccgacgggtg gccgatcacc tcgtcgcgcg
    38641 tgtagcccat cttggtcagc caggcatcgc tgacagacag caggcgaccg ttgccgtcga
    38701 tggactgcag catcgccggc gtggcctcgt agaggcgccg caggcgctcc tcgttcaacg
    38761 acagctgggc cagcaggtcg tggtgagccg tcacgtcctc ggccgaaccg acgtagccgg
    38821 tccttgcacc gctggcatcg tggagggagc gcgctcgcga gtgcacccga cgcagttctc
    38881 cgtcgggacg cacgacacgg aactgcaggt cgaattcgct gccgtcggcg ctgaagcgct
    38941 gccactcggc ctgcacctgt tcgacgtcgg caaggtggat cgcctgcaac cagccgtgac
    39001 cgaggctgtc ggccagcgtc aggccgaaga tctgctgcca acgcgcattg gtgtagcaac
    39061 acgcgccatg cacgtcggtg tagtagatgc ccagcggcga gctttcgctg agcacccgaa
    39121 agcgcgcctc gctggccgtc agccagtcga gggtctgctg cgctccctgc agggtcagac
    39181 gcgcctgaag cgcgcgcgag gccacctggg ccaggcagcg cagcaactca cgctgggttt
    39241 catccagctg gcgtggctgg cggtcgatga tgcacagggt gcccacgcag aggccgtcgg
    39301 ccaggcgcag gggcacgccg gcatagaagc gtatgcccgg ttcaccggtg accagcgggt
    39361 tgtcggcaaa gcgcggatcg cgcagggtgt cgggaatctc cagcaggtcg tcgcacagga
    39421 tggcgtacga gcacaggccg atgctgcggg gaacctcggt caggtcgggc aggccgacct
    39481 tggccttcag ccaatgccga ccgcggtcgg ccaggctgat cagtgcgatc ggcgccgcgc
    39541 acagcatcgc ggccgcacgg gtcagcgcat cgaactcggg ctcgggggcg gtgtcgagaa
    39601 cttcgagggc atccagggcc gccaggcacg cggcttcgtt gtcacgatct ctggctgaaa
    39661 gcacggggaa ctacctcgat tcagacccgc accttgaatg ccgcaaccga ctggaccagc
    39721 tcgtgggcct gcttgctcat gctttccgtg gccgcagcgc tctgctcgat cagcgcggcg
    39781 ttctgctgcg tggtctggtc catgccggac accgcctggc ccacctggct gatgccggtg
    39841 ctctgttccg tggtcgccgc gctgatctcg gccacgatgt cg
//
LOCUS       DAOORV010000015        37476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1269_length_37476_cov_7.828278, whole genome shotgun sequence.
ACCESSION   DAOORV010000015 DAOORV010000000
VERSION     DAOORV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 37476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_37476_cov_7.828278"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="argH"
                     /locus_tag="PKV56_03715"
     CDS             complement(<1..1004)
                     /gene="argH"
                     /locus_tag="PKV56_03715"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOX66950.1"
                     /translation="MSSNQLDKKSEAWSALFSEPMSDLVKRYTASVFFDQRLWRADIA
                     GSLAHAEMLCHQGIISIEDRASIERGMTQITEDIEAGRFEWKLDLEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRGEIDAMLPLLGAMQSALVDVAEKNVETILP
                     GFTHMQVAQPVSFAHHLLAYVEMFSRDADRLADVRKRVNLLPLGAAALAGTSYPLDRE
                     RVARTLGFDGVCQNSLDAVSDRDFALEFSAFAAIAMVHISRLAEELVLWMSQNFGFID
                     LADRYCTGSSIMPQKRNPDVAELARGKSGRVVGHLMGLITLMKGQPLTYNKDNQEDKE
                     P"
     gene            1091..2161
                     /locus_tag="PKV56_03720"
     CDS             1091..2161
                     /locus_tag="PKV56_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX66951.1"
                     /translation="MTPVTPANPDDPTSTNLSGFGALTTPERPPRPEPTLFDVCHVGV
                     VLRATLFVHAALATGVLFVAPTMRGWALQFALGAGVAMPAVLAWLISACALKAPLSRM
                     SAAGQASVSAVLGAACGCAGWALAMQAGPGDLERIGPFAPTAAGAALALVMHQWLRMR
                     ALARTPADTTAQLAELQSRIRPHFLFNTLNSALALVRLDPQRAEHVLEDLAELFRVAL
                     TETGGSVTLDEEVELAKRYLAIEQIRFGSRLAVTWDLDPDAGSARMPPLLLQPLVENA
                     VRHGIEPADDGGTIRVRTRARGDRAVISIANSVTGTASRPGQGIALRNVQDRLRLMHD
                     VNAQFESRRDGDAFRVQIVVPL"
     gene            2158..3009
                     /locus_tag="PKV56_03725"
     CDS             2158..3009
                     /locus_tag="PKV56_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOX66952.1"
                     /translation="MNDAAENSTGLLRVLIVDDEELARLRLRALIDDCSQPRATVVGE
                     VANAAQALVWLQSHGQGPASPGSGRACDLVLLDVQMPGRDGTQLAEELRALPLPPAVV
                     FVTAHAGHALKAFDLEAVDYLTKPVRRERLQLALQRVSARLGQPTTVPGELGSQPDPV
                     LIVSERGRVVRVPVREVLYLKAELKYVTLRTASHTYVLDDALSDLEQRLGERFLRVHR
                     NALVARSAISALERRTMTDGDGDASDEGHGPNECWAVRVAPVDEWLAVSRRQASAVRD
                     ALAASGL"
     gene            complement(3043..5850)
                     /gene="ppc"
                     /locus_tag="PKV56_03730"
     CDS             complement(3043..5850)
                     /gene="ppc"
                     /locus_tag="PKV56_03730"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOX66953.1"
                     /translation="MPKKTPHADAADKNRPLVEDIRLLGRILGDVIREQEGKDAFELI
                     ERVRQLSVGYRLKRDAQAGRALDRLLKNLSGDQTVSVIRAFSYFSHLANIAEDRHNVR
                     LRELHEQKGELREGSLAMTFERLAAADVRAADIVRTLDHAYISPVLTAHPTEVQRKSI
                     LDAERAIAELVEHRDDIHTARDRADNEALIRARVTQLWQTRMLRNTKLTVADEIENAL
                     SYYHSTFLRQIPRMYRDIEAALPGHPIASFFRMGNWIGGDRDGNPFVTASTLQVALAR
                     QSETVLRFYLTEVHQLGGELSISATLAPVTAAMNELSESSPDHNPHRDDEPYRRALVG
                     IYARLAATLQDLTGTEALRHAVPPQNAYLSASDFLADLRVIEESLNAHHAQALAAPRL
                     RPLMRAVQVFGFHLATVDLRQSSDKHEAVLAELMRVAKIEPDYSALDEAARRALLLRL
                     LDDARPLRVRAAAYSDLAIGELAIFESAVEMRRRYGPDALRHCIISHTEEASDLLEVL
                     LLMKETGLMRGTLDDAAVADLIVVPLFETIGDLRNAEPIMRSFYGLPGITALVKRSKL
                     GDFAEQDIMLGYSDSNKDGGSFTSSWELYRAEIALVELFAELGRDHGITLRLFHGRGG
                     TVGRGGGPSYQAILAQPPGTVNGQIRLTEQGEVIASKYAHPEIGRRNLETLVAATLEA
                     TLLHTKKASRQGAPKSFLDAAAAISEASYAAYRKLVYETPGFTEYFFSATPIREIAGL
                     NIGSRPAARKATNAIEDLRAIPWGFSWGQCRVALPGWCGFGSAIEKFLTTDADARQER
                     LALLQRMHKQWPFFQTLLSNLDMVIAKSDLGIAARYVELVEDKKLGKRIFALIQAEWT
                     RTNDALTLITGHKDRLASNPSLARSIQHRFPYLDPLNHLQVELMRRHRQRREGDPGIE
                     RMQRGIHISINGVAAGLRNTG"
     gene            5964..6902
                     /gene="hemC"
                     /locus_tag="PKV56_03735"
     CDS             5964..6902
                     /gene="hemC"
                     /locus_tag="PKV56_03735"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOX66954.1"
                     /translation="MTSHIIIATRESRLALWQAEHVRDLLGSRFGLRVELLGMTTRGD
                     EILDRTLSKVGGKGLFVKELETALEEGRAQLAVHSLKDVPMDLPDGFELAAVLEREDP
                     RDAFVSNTHASLADLPHGAKVGTSSLRRVVQLRALRPDLLVEPLRGNLDTRLRKLDEG
                     QYDAIVLAAAGLKRLGLASRIRSEFDTDQMLPCAGQGALGLEVRSDAAALRTQLATLA
                     HAPTALAVHAERAVSRALGGSCSMPLAAHAVWAGSALSLRAAVGHPENPDAPLLRAAV
                     SAEVQTEEQARALGEQAAAVLRDSGATLYLAAVATH"
     gene            6961..7752
                     /locus_tag="PKV56_03740"
     CDS             6961..7752
                     /locus_tag="PKV56_03740"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOX66955.1"
                     /translation="MRVIVTRPAAQAMPWVRSLEKHGLKAVALPLIDIGPALDTRSVT
                     EAWQQLGQARLAMFVSANAVEHFFALRPIDTVWPTNVQAGSPGPGTTGALLDAGVPSS
                     LIVEPAADSAQFDSESLWERLRDQDWRAAPVLVVRGDGGRDWLADTLRAHGAEVSFVS
                     AYRRTAPTWNDKMQTLLGEALTRPDEHLWYFSSSESIDHLMDHLANSRQQPTPSTPIG
                     LPIGARALATHPRIARRAEQAGFAPVWLCPPTLADVVACIQSAAP"
     gene            7749..8930
                     /locus_tag="PKV56_03745"
     CDS             7749..8930
                     /locus_tag="PKV56_03745"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOX66956.1"
                     /translation="MTDHPTAAVPAEPTTPLPVAEAPSPAGASVRPETSFGAMRWWSA
                     LAAVLAVMAGVSLVMAWKTQQRVKSVEQQLVRRLEDSQLQASEARLLAKQSQDSVLAA
                     AAKVALLEARVAEVAVQRSQLEDLMQSLSRSRDENMLVDIDAGIRVAMQQTAITGSAE
                     PLVAMLKQADERLARYDQPRLEGVRRAIARDLDRVKAVGVIDVPTLSIKIDEVVRLID
                     ELPLLSNAEQRPASPRAKAIAAAASSAVPVVAASGSAAGVRAASSPSTAWWQTWDGSW
                     AAISERVWSEVKSLVRVTRIDHPESMLLSPDNAFFVRENVKLRLLNARLALLSRQFDT
                     AQSDLQVVQASLDRYFDRSSRRTALATDLLRQVTTQARQVTVPKPDDTLVALAAATAG
                     R"
     gene            8947..10194
                     /locus_tag="PKV56_03750"
     CDS             8947..10194
                     /locus_tag="PKV56_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOX66957.1"
                     /translation="MRSVIWFVLLFGAAVVAASTLGANDGLVSVYAGTWRFDISLNLF
                     LLLLVGSCFALVSLIHAANSLIGLPERARLWRVARRERTAQAALREALAQYFSGRYSR
                     AKAAAQQALTIQDDTPELKPDTEFSVLGLLLAAGSAHRLQDRSKRDEHLARAFKLAQG
                     STAARSAEEGARLMAAEWAIEDRDAPRSLILLSELPPGVARRTQALRLKLQAARLGQQ
                     PQEALKTARLLVKHQGLSKAASQGLLRSLAFECLDTAHDIDQLKRIWLSLDAADRRDN
                     FVAARAAVRAAHLGSTDDARSWLRPAWDRIEETSDDERAALSHALVSAVAGIGIDWLQ
                     RLESARLAFPRDAAVAHAVGTALAERQLWGKARQLLETAAADADLPITAKRMAWLTLA
                     RLAENEGQPERAAISYRNAALLR"
     gene            10229..10305
                     /locus_tag="PKV56_03755"
     tRNA            10229..10305
                     /locus_tag="PKV56_03755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10263..10265,aa:Arg,seq:acg)
     gene            complement(10901..11815)
                     /locus_tag="PKV56_03760"
     CDS             complement(10901..11815)
                     /locus_tag="PKV56_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaL protein"
                     /protein_id="HOX66958.1"
                     /translation="MKAWRSLLNGLATRQNLPIVAAAALLAAAVWMPPVMLQRATYQY
                     LLAFDVTQSMEVDDQALDGAAVTRLAFARAAAREALRRLPCGSRVGWAIFADYRVMPL
                     VLPIEVCGNYDALLASLERIDGRMRWANASNVGKGATWLVRTAKAIGPDTRVVFFTDG
                     QESPPLRSQEVKPPMQDITPGEAGGWLIGVGGDVPARIPRFDGEGRPLGYWAAGDVVQ
                     RFGPGTPGTAHEHLSELREPHLQALGKLLGLGYRHLTAPDVLADAMLDPALARPQPVA
                     TDLRWVPALLGLLVLSVRFLPDVVRRRG"
     gene            complement(11812..12378)
                     /locus_tag="PKV56_03765"
     CDS             complement(11812..12378)
                     /locus_tag="PKV56_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaK protein"
                     /protein_id="HOX66959.1"
                     /translation="MRRISMHAAFALATTAALAGAGFNGLRWQQARQLNTTIAAAAQA
                     LAPSASAAESTVSRDAPREVRLAQATAWSQAGAFDAAFKHFAGLIEPGRSDAISRHAQ
                     YNLGNMYVRQGLASSTPADTGPLLELAKQRYRDLLRADPQDWDARYNLERTLRAAPEE
                     QETTAEAPNVPVERRNVALRGMIAGDLP"
     gene            complement(12375..13382)
                     /locus_tag="PKV56_03770"
     CDS             complement(12375..13382)
                     /locus_tag="PKV56_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOX66960.1"
                     /translation="MGLDFLQPLWLLLMPLAALPLLRRRRDTLRFSSVGWLPSDPWGR
                     AAGLLWRAFACGAMAGAVIGLAGPGQSGTQVQRTGKGAEVLILMDRSASMDANLLPKD
                     VKAGGQFNASMPTKIQVVRELLADFVKRRPDDRFALMTFSTSPMLVAPFTQDHATVQA
                     GLQATAIGRGLPDTLMGRALLAAIDQFEGRPYSGSRIVIVVSDGGAQLDDTQRQRIAT
                     GLARHRIGLYWIYIRSGPNSPDLTRTDAGTMDSEEEGALHAFFKTLATPYRLYQTDDP
                     EAMATAIAEIDRQQNFPLTFIQQVPRLDFSAGCFGAALLCCLGLLTCRALQLQTWRSL
                     T"
     gene            complement(13385..14269)
                     /locus_tag="PKV56_03775"
     CDS             complement(13385..14269)
                     /locus_tag="PKV56_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium incorporation protein MxaA"
                     /protein_id="HOX66961.1"
                     /translation="MNLHRTLLAAALLALMRSVAAESPPTPAAPAATVEQPRSFGHVL
                     GDVLTQRVLLSHAGRPVDAGALPAADRVGLYLERRTPRVEEDALGRRWLAIDYQVVNA
                     PRALTAAALPALSITTSAGPLQVVAWPVSIGPLTPPATFDQGALQPLRPDRPVLPVPT
                     EGLERQFKLSLTALAVVLLGWAAWWAARNAREARRLPFARAWQQLRQADPASTEAWRA
                     LHRALDATAGRVVHGRTLPRLLADAPHLQPLAPQLEAFFRHSEQRFFASEAGTAPADL
                     SLRALGLALREAERRHHR"
     gene            complement(14266..15156)
                     /locus_tag="PKV56_03780"
     CDS             complement(14266..15156)
                     /locus_tag="PKV56_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaS protein"
                     /protein_id="HOX66962.1"
                     /translation="MAANLAPQEFHYRLPATLGGQRPGAHRGSSLGAGQAFAAHRRLL
                     DHPDPRRIDLRASLRDVRGDWLVRIQRQRVAVPVHAVVDVSASMHFGALRSKLEVVAD
                     FVEALGHSAFRSGDALGLLAFDHAQRDDLFMPARHSRGAGIVMADALRRCEAEPAYQH
                     DRTHELTAQALQRTVAPLAGRPGLVFLVSDFHWPLAPLHGVLELLAPACVVPLVVWDP
                     AETEPPAAHALVAVSDAETGVRRTLWMREPLRQRWADAVATRREALRALFDAHGLVPF
                     HLHGCFDAEALTRHFMEAVA"
     gene            complement(15156..16175)
                     /locus_tag="PKV56_03785"
     CDS             complement(15156..16175)
                     /locus_tag="PKV56_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX66963.1"
                     /translation="MNPERELQDWREQALCLEREVAKAVIGQAATIRLINVATFARGH
                     VLLEGDVGVGKTTVLRAFSRAVGGDFERVEGTVDLMPGDLIYHTYVDAEGRPRIDPGP
                     LLRHGERLTTFFFNEINRARPQVQSLLLRAMAERTVSAFNREYRFPHMTVFADRNKVE
                     KEETFELASAARDRFMFELNMSKPVDRGIRRALVFDTAFHDADQLIEQVPAAMVRADQ
                     LNAIGALIQRSVSSSEAIESYVLDLWEASEDPLRFGVQLDGVDMRRLVLAGASPRGMS
                     ALLRAARVVAWLQGRTHLLPDDVLAVLPSVLAHRVFFTPVYELRRAELAPALVQKIID
                     QVAAP"
     gene            complement(16284..16970)
                     /locus_tag="PKV56_03790"
     CDS             complement(16284..16970)
                     /locus_tag="PKV56_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX66964.1"
                     /translation="MSILLVDDHPVVREGYRRLLERQPGFTVCAEAVDADSAYEAYCR
                     HHPDVVVMDMLLPGASGLDALRHIRQRDADARVLVVTMHSGASMALKVLEAGAWGYVT
                     KSSGPNELVRAVTAVAQGEQALCDDTRQAIATQRLADPIAVLDDLGAREVEILRLVAS
                     GHTSDAIADALNLSEKTVRNYHYAIKSKIGVRTDAQLVWFALSVGLVRLDDVTAALPF
                     PVPTTGGTHD"
     gene            complement(16976..18367)
                     /locus_tag="PKV56_03795"
     CDS             complement(16976..18367)
                     /locus_tag="PKV56_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX66965.1"
                     /translation="MQLLPQLVTRLFVVTALCLAAAIAWIVFDAHRSVEREAELSAAR
                     VGQHLEALYWQEQLWRGRGRAGLNTPAPEWQSFATLNLISPGVCVTFALRGDAQQTLC
                     SQTASLGPRAPDWFVSAYGTLFPHDTSVERPLFARDPGSGFIVNTPDRGAAIRQAWRQ
                     VSWVAGIAAGMAVAIALLAALMIGRTLRPARAIVDGLQRLEQGDLAWRLPSFRTAEFA
                     RIASALNQLAGRLARTNEDRNALTTRLLQVQENERRALARDLHDEFGQCLTATAALAA
                     SIEAGAPAGRPDIVEEAQAIGRTQARMMKTLRSTLVRLRTQDIEEIGLEASLRQLVSE
                     HNLQTTSRSVFQLSVDGEIAALPRPIAIDVYRIAQECLNNAARHGSPTQVALRVAQEG
                     REVAVTVQDDGGGDAARIGQASSGGHGITGLRERLAALGGTLAIHNASGGIRVAAIIP
                     LAANDPSARRAAA"
     gene            complement(19132..19308)
                     /locus_tag="PKV56_03800"
                     /pseudo
     CDS             complement(19132..19308)
                     /locus_tag="PKV56_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
     gene            complement(19327..20445)
                     /locus_tag="PKV56_03805"
     CDS             complement(19327..20445)
                     /locus_tag="PKV56_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOX66966.1"
                     /translation="MEQAVIWTIPFLALAVILEMTVSFVRKRKSYRTNDTLANLSQGL
                     ISQLVGVCTPLLQIGIYAMVFSALGHQVESPFWHSWYGIALAVVMFDFCEYWLHRVGH
                     EVAIFWAAHVVHHQSEELNICTALRQESQNALLGWPFYLPLAIAGVPPVMFGFVLLGV
                     QYYQIWAHTEQIGKIAWLDGVLTTPSNHRVHHAVNDCYLDRNYGGLFILWDRLFGTYA
                     AETEPCVFGTRSPVRSFNPLRAITQVYGALARDAWYTQRWQDKLRVLLMPPGWRPDDV
                     AQRFPQPPFDLHREQYDPPASAAARAAAVVLFLTTAVGCGFFLWDAEAMDMSARILWT
                     ALLMTGLCGVGWLLESGAGAAAQPNDLPRVSGGLSPHE"
     gene            complement(20453..20842)
                     /locus_tag="PKV56_03810"
     CDS             complement(20453..20842)
                     /locus_tag="PKV56_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66967.1"
                     /translation="MKKWSVCLLLICAAGSAAANWVRVRGDGVVTVLADPSTIVRSGD
                     MVKMWSVINYAQPRIAPDGKAYSSSRQQLEFDCVENQSRSLFFSRHTDFTGWGDVIYR
                     NESPGSWSPAPPGSVGAALRKFACHLP"
     gene            complement(20925..22055)
                     /locus_tag="PKV56_03815"
     CDS             complement(20925..22055)
                     /locus_tag="PKV56_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOX66968.1"
                     /translation="MPGLLPNVDPDGLLEYSVVYTDRAINHMSKRFQQVMKDISATLK
                     EVYHAKSAVVVPGSGTFGMEAVARQFATGRKALVIRNGWFSYRWTQIFEMGNIPAQST
                     VLKARRTGPGKQAPFEPAPIDEVVATIKELRPDVVFAPHVETSAGMILPDDYLRAVAD
                     AVHSVGGLFVLDCIASGTIWVDMAATGVDVLVSAPQKGWSGSPCSALVMMSELARTRI
                     DSTTSTSYACDLRKWMQIMEAYEGGAHAYHATMPTDALARVRDVMLETRDYGFDKVRD
                     EQQQLGNAVREMLTRRGFKSVAAPGFQAPGVVVSYTEDADMQSGKKWLGLGLQIAAGV
                     PLQCDEGPDFQSFRIGLFGLDKLHNPARTVKNLEDALDRLQS"
     gene            complement(22253..22972)
                     /locus_tag="PKV56_03820"
     CDS             complement(22253..22972)
                     /locus_tag="PKV56_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66969.1"
                     /translation="MASSIKTGAALLGGVVCLTLVGCGGPGGDTSLFPLDKGHRWAYK
                     VATELENNTVQREEMTLSSLGRVDFDGQPAWVRRSDSGANYWLRSDDTGVYRVASRSD
                     IEDEPRPDPVRRYVLKRPYAVGTSWQSATTAYLLEHKQDFPREVRYKHPVIPMNYVIE
                     AVNQKVDTSAGKFDGCLRVKGQALIRLFADPVVGWRDIPLTTLEWYCPGVGLVRLERD
                     EPGKATFISGGRIQMDLADFR"
     gene            complement(22978..23472)
                     /locus_tag="PKV56_03825"
     CDS             complement(22978..23472)
                     /locus_tag="PKV56_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66970.1"
                     /translation="MTLALILSFAALALLCVLALLWSRWPAWLKGILVVGVTVFYFYA
                     DSVVHQLSGWPSPDALPERFVLLAAVIEEPSARTDGALYVWVNAIDNGKPTAQPRAYR
                     LPYSKDVHSMLNEGIKKVRQGVSQMGTSEPKVGKGGTSWLRPGNDEQVVKIRDLPVPQ
                     LPEK"
     gene            complement(23469..24083)
                     /locus_tag="PKV56_03830"
     CDS             complement(23469..24083)
                     /locus_tag="PKV56_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66971.1"
                     /translation="MRKPPSARLSREAEVDPFYDMLFNMLIAFVFCFIIALLAMNPKA
                     LKAGDIPSKAEYIVNVSWPDGNPNDIDTWVQDPGGNLVWFRAREAGLMHLDRDDRGLS
                     GDVILINGKEIVNPLNQEVVTIRGIEPGEFTINLQYYETKNGEAVEATISVIKVNPRA
                     DVVYYGQVQLARKGDEATAVRFTVLPDGQVTNVNTLPKKLVSNP"
     gene            complement(24087..24848)
                     /locus_tag="PKV56_03835"
     CDS             complement(24087..24848)
                     /locus_tag="PKV56_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66972.1"
                     /translation="MSSMMNRLLMSSRRQPAHGSASAAREPMTEPLWLEWLTLAGLLI
                     FASWLLGVRGVFAMLLQADPTGITLVIMVVFAGATLWCGARSRTLQQQRQAMLAAQLA
                     GPGRATAEAGWAAAYWSAIAGPARDSGAPLDLLLDKTHGPHSTAWWVNGIQLKLGLLG
                     KVIGFSILALQIGHTTSFDPSQSQELLRSLTTGLGVALLTTMVGLVGNIVLGLQLTRL
                     DRYADAFVADCQRFGLSVEADVPPRTLEAVVSFTG"
     gene            complement(25185..27395)
                     /locus_tag="PKV56_03840"
     CDS             complement(25185..27395)
                     /locus_tag="PKV56_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX66973.1"
                     /translation="MAASHRGIKPASVSVASASATGLQSADLRSHLAQIEESHLRCTA
                     LGLSRIERPDNEPLMRGDLNVARERNQRLSTHAAPVMEMLFEQIIDSHSMIVLTDAQG
                     TILHSMGDNDFLERADKIALAPGANWAEHSKGTNAIGTALFAEAPTVVHAGEHFMHAH
                     NFLTCSATPIFDPRGNMLGVLDVSGDQRTYHRHTMGLVRMSARMIENQWLHDDYSNRL
                     RLHFHRSVSYLGTLVEGVVVVGSDGKIIGANRSALELLGLSGASLRMHSLTSLFGASA
                     ASVFDHFRSPLAVPMQLNGPCGTPFFVSARFVWPPRAVVSGAVNWIDVSGAEVDGDAV
                     EPVALPRAPDEMPEQQSSERMAKAAAAPRKLAGIGLARLETGDARIAATVEKVRRVLN
                     RDIPLLILGQTGTGKEVLARAAHHDSQRARQPFVAVNCASIPESLIESELFGHEEGAF
                     TGARRKGSIGLIAQADGGTLFLDEIGDMPMALQARLLRVLQERCVTPLGAQKAIPVDI
                     AVIAATHRDLRGMIERHQFREDLYYRLNGLVVRLPALCERTDLAALVKRILAAEAPER
                     PPQVCPSVMALFRRYGWPGNVRQLVNVLRTAAVMAAGEAQINETHLSEDFLEDVQRGT
                     APRAIASSTAEAPTTSVRNTGIPEPFGARSEVSERGGAFAVRNAAEASDGDAPVPQVS
                     PPAAAERSAKTLEEGEVEMIRNALDAARGNISEAAKRLGISRNTIYRKLRWNQS"
     gene            27778..28206
                     /gene="pedF"
                     /locus_tag="PKV56_03845"
     CDS             27778..28206
                     /gene="pedF"
                     /locus_tag="PKV56_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HOX66974.1"
                     /translation="MGLGTSAAWAHGDVTPQSVDTKALPPLGADWLAENPYRGNAAAV
                     KIGDSAYNQNCARCHGLQAISGGIAPDLRKLDNDCVSLQDVKKKAECVKDVDVYYLGS
                     IRNGKVRNGAVYMPKFEGVMNQEAMWSIKAYLETVRERPL"
     gene            28203..29069
                     /locus_tag="PKV56_03850"
     CDS             28203..29069
                     /locus_tag="PKV56_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX66975.1"
                     /translation="MTTHGGPADWTRRRCLQAAAGASALALLPARADDDEPDPLEKIR
                     KRGSLVVGLYKDLPPFHVAGAGIDVDLSQALAADMGLKFTALPFDAGENMQDDLRNMV
                     WKGHYMGYGPADVLMHVPVDKALMNSSPQVEIFGPYYRERLAIARNLEKVPVLDSLSR
                     IAGLQVAVPGQSLSGWLLLGAENGAYREQMQTRWADGAEAAQALMRGDVPLAAGMVSE
                     LESVVRGDTRFAIEPLPLPRSPREGWAVGMAVKKDSIRLAQALQSSLNNLVKNGKVAE
                     LFAKHSVSWKPV"
     gene            complement(29104..29862)
                     /locus_tag="PKV56_03855"
     CDS             complement(29104..29862)
                     /locus_tag="PKV56_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX66976.1"
                     /translation="MLPWPLPALLTWAFAWAVFALLSAVAAPPIVALACAAVVGALPA
                     VLSPWPLTRWRRLFIALGFPLSLALSGSAHDVPAWAWLLPLTVLALLYPLNAWRDAPF
                     FPTPRGALEGLQRLVPLPDAARVLEAGCGLGDGLRELHAEYPTARIEGIEWSWPLRWA
                     CQRRCRFAGVTRGDLWKTDWSAFQLVYLFQRPESMPRAVEKACRELPSGSWLASLEFE
                     ARQLQPDAVHACPDGRTVWLYQQPFTRRRESSKS"
     gene            complement(29894..30613)
                     /locus_tag="PKV56_03860"
     CDS             complement(29894..30613)
                     /locus_tag="PKV56_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOX66977.1"
                     /translation="MSTYTAKGQLDNTSMLKQYSPLVRRLAHQMIAKLPANVELDDLI
                     QVGMIGLADALTRFDAAQGVQFETFATQRIRGAMLDELRGNDYLSRGTRKQQRTIESA
                     VHKLEQRMGRAPAESEIAKEMGITLTEYQELLGKVRGTQLVYLEDMSGDDGDHDYLDR
                     HVADESFNPLAQLQDQRMREALVAGIKVLPEREQYVMSMYYEHDMNLKEIAAVLKVTE
                     SRVCQLHSQSIARLRVKLREY"
     gene            complement(30630..31427)
                     /locus_tag="PKV56_03865"
     CDS             complement(30630..31427)
                     /locus_tag="PKV56_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HOX66978.1"
                     /translation="MTPSFTPSSSSMPLDQADGLRRLFAHAQVRFVPVVSNPHMAFGG
                     VMLERLCSGLAEQGCHTLVVDAGENAPGPNELAEVDLAECIEVLSQEVSYLAARGLPL
                     RYVDSNGSTASFLEAVSLAAPEADVVLVHASATDLCRLFMRRESSTRPRPLLLADDRP
                     VSVTHAYAAMKLLTQRAGLLVHDLLLSAAPSSPRAERIAMQIATCADDFLGAVLRDWM
                     QIDPASDARSAPTPAMRRWVRDTLAPATGELAVLPTAANEHHAHALN"
     gene            complement(31490..33202)
                     /gene="flhF"
                     /locus_tag="PKV56_03870"
     CDS             complement(31490..33202)
                     /gene="flhF"
                     /locus_tag="PKV56_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOX66979.1"
                     /translation="MNVKRFTARTSRDALALVRQALGEDAVVLSTRPSAEGVEVLAMA
                     PESMAQIERMATDSTPAAAPTARTPAAQRPAAGRPGPGRPGVARTQLAARVEPQPAAD
                     ATTVEDDVERLSMSTLSFQDYVRERMLKRRQVALQAESAAARAAEPVVEPVPGPLATR
                     MADAPVARSTPPASNGPSRVAQPTPGHAPVLSALHSDTDPMYVNAMPVLHEPPVLHEE
                     VRTLAEARGRARVAPAATAPLAADASAARRDQQDMMSELRSMKGMIEERFGALAFMEK
                     LQRQPAQARLTQKLIDCGFSPALIRKLAEGMGTEVTDEMGWAAGVLERNLLSSDREPA
                     LEDQGGVYALVGATGVGKTTTTAKLAAAFATRYGAAHLGLITLDAYRVGAHEQLRAYG
                     RILGVPVHTAHDRASLEDLLELLAGKKMVLIDTAGMAQRDTRTRELLEMLSHRSIHKL
                     LVVNASAQGETIEDVLISYHASTCRGIVLSKMDEAVKLGPALDALIRHKLTVVGVANG
                     QRVPEDWHRLSSHALIHRALRPTVGSAYRMDSSEVNLIFAAPQHQSHPLQQAVPHDAM
                     ALHA"
     gene            complement(33199..35283)
                     /gene="flhA"
                     /locus_tag="PKV56_03875"
     CDS             complement(33199..35283)
                     /gene="flhA"
                     /locus_tag="PKV56_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOX66980.1"
                     /translation="MNPLQQLKGWLGPNASAIKALMAPVFIVMVLAMMVLPLPPFALD
                     LLFTFNIAMALMVMMVAAYMVKPLDFAAFPTVILLTTLLRLSLNVASTRVVLMEGHTG
                     PGAAGAVIESFGHFLIGGNFAVGLIVFAILVVINFVVVTKGAERIAEVGARFTLDAMP
                     GKQMAIDADLNAGLIDEKEAKRRRAEVGDEAEFFGSMDGASKFVRGDAMAGLLILVIN
                     IIGGFIIGVAQHNLSAAEAADSYIVLAVGDALVAQIPGLLISVAAAMVVSRVGKGNDV
                     GTQIAAQMFNSPRALAITAGVIGTLGLIPGMPHVVFLLIAASLGYAAYWMRERDRVAR
                     QAPPPAPAMAAEANAEATWDDLLPIDTLGLEVGYRLISLVDKAREGDLLARIKGVRRK
                     FAQDVGFLPPPVHIRDNLELKPSMYRLTLRGAVVGEGEAFPGMWLAINPGGASTNLIG
                     TRTTDPAFGLPAVWIEERQRESAQMNGFTVVDCSTVVATHLSHLMQLHAAKLLGRVEV
                     QNLVEHVTKLAPKMIEDVVPKMVAIPALQKVLQLLLEEGVHIRDMRSIVESLAENAST
                     VTDPAELARRIRIHLAPSIVQQIYGPSKELDVIALEPDLERLVTQALNSPHGAALDPG
                     VADTLARHAADSAKRQEDLGLPACLLVPDLIRAPMARLLKRAAPRMRVLAHSEIPETH
                     SIRIGSVIGGTA"
     gene            complement(35280..36461)
                     /locus_tag="PKV56_03880"
     CDS             complement(35280..36461)
                     /locus_tag="PKV56_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HOX66981.1"
                     /translation="MADQDAQDRNLPASARKLKKAREEGNVPRSRDFGHFAALAGGGL
                     GLVVMMPTLVGWTREGLSRGLSFGAGSRGAPSVMFDLLADLLAMMLWVVVPMGLWMTA
                     LGIASALAVGGWNWTLKPLMPKGTPFKPIAGLGRMFSKQQMVDATKASVLALLLGTVG
                     CVYLYSHVDEFRGLVSMPLSAGLTQAGSAIQGGLWLLLGVLALFAVVDAPLQRYLNAS
                     KLKMSAQEQKQEYKESEGSQEVKGRRKQLMREMSRRRMLAAVPTADLVVMNPTHYAVA
                     LKYDEARMGAPRVVAKGADILAMTIRDTARAAQVPVLQAPVLARALYAHAEVDREIPT
                     ALFAAVAQVLAYVYQLRAAMSGRGAMPPDLPELSVPPELDPHNDPDYVPPAPSTEKDA
                     E"
     gene            36795..>37476
                     /locus_tag="PKV56_03885"
     CDS             36795..>37476
                     /locus_tag="PKV56_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOX66982.1"
                     /translation="MSTPDKKAAPRRRFLQRAATGAVATGALAAPMVSRAQTTSLRFQ
                     STWPAKDIFHEYASDFAKKVNDMAGSRLKIEVLPAGSVVPAFQLLDAVSKGTLDGGHG
                     VIAYWYGKNSALALWGSGPGFGMDANTVLAWHNYGGGKELVAEIYKSLNLDVASYMYG
                     PMPVQPLGWFKKPVAKVEDMKGLKFRTVGLAVDVFTELGTAVNPLPGGEIVPALDRGL
                     IDAAEFNNA"
BASE COUNT         6077 a        12591 c        12811 g         5997 t
ORIGIN      
        1 ggctccttgt cttcctggtt gtccttgttg taggtcagcg gctggccctt catcagcgtg
       61 atgaggccca tcaggtgacc caccaccctt ccgctcttgc cacgagccag ctcggccacg
      121 tcagggttgc gcttctgcgg catgatcgaa gagccggtgc agtaccgatc ggccagatcg
      181 atgaagccga agttctggct catccacagg accagctctt cggccaggcg ggagatgtga
      241 accatcgcga tcgccgcgaa ggcgctgaac tcgagcgcga agtcgcggtc gctgaccgcg
      301 tcgaggctgt tctggcagac accatcgaag cccagcgtgc gcgcgacccg ttcacggtcg
      361 agcgggtagc tggtgccggc cagtgcggcg gcgcccagcg gcaacaggtt gacccgcttg
      421 cgcacgtcgg ccagccgatc ggcgtcgcgc gagaacatct cgacgtaggc cagcaggtga
      481 tgcgcaaagc tcactggctg cgcgacctgc atgtgggtga atccaggcag gatggtctcg
      541 acattcttct cggccacgtc aaccagcgcg gactgcatgg cacccagcaa cggcaacatc
      601 gcgtcgatct cgccgcgcag ccagaggcga acgtcggtcg ccacctgatc gttgcgcgag
      661 cgaccggtgt gcagtcgctt gccggcgtcg ccgaccagct gcgtgaggcg tgcctcgatg
      721 ttcaggtgca cgtcctcgag gtccagtttc cattcgaagc gaccggcctc gatgtcctcg
      781 gtgatctgtg tcatgccgcg ctcgatgctg gcgcggtcct cgatgctgat gatgccctgg
      841 tggcaaagca tctcggcatg cgccagtgaa ccggcgatgt cggcgcgcca gaggcgctga
      901 tcgaagaaga cgctggcggt gtagcgtttc accagatcgc tcatcggctc ggagaacagc
      961 gccgaccagg cttcggattt cttgtcgagt tggttggacg acatgggctt gcgacgaatc
     1021 gcgatgcgtg tcgtcggcgt tgaggtggca gggtttgtag acaatcagtc cagcacgatc
     1081 gattctatcg atgacccctg tgacgcccgc caatccagac gaccccacat cgacgaacct
     1141 gtcggggttc ggcgcgctga cgactccgga gcgcccaccc cggcccgaac ccacgctgtt
     1201 cgacgtctgc catgtcggcg tggtgctgcg cgccacgctg ttcgttcacg cggcgctggc
     1261 caccggggtc ctgttcgtcg cgccgacgat gcgcggctgg gcactgcagt tcgccctcgg
     1321 cgcgggggtc gccatgcccg ccgtgctcgc atggctgatt tccgcctgcg cgctgaaggc
     1381 tccgttgtcg cgcatgtcag cggcaggaca ggcctcggtg tctgccgtgc tcggtgcggc
     1441 ctgcggctgc gcgggctggg cgctggcaat gcaggccggg ccgggcgatc tcgagcgcat
     1501 cgggccgttc gccccgaccg ccgccggtgc cgcgctggcg ctggtgatgc accagtggct
     1561 gcggatgcgg gcactggcgc gcacgcccgc agacaccacg gcacaacttg cggaactgca
     1621 atcgcgtatc cgcccgcact tcctgttcaa cacgttgaac tcggcgctcg ctctcgttcg
     1681 cctcgacccg cagcgcgccg agcacgtgct tgaggatctg gccgagttgt ttcgcgttgc
     1741 gctgactgaa accggtgggt cggtcacgct ggatgaagag gtcgagctgg cgaaacgcta
     1801 cctcgcgatc gagcagattc gcttcggcag ccgcctcgcc gtcacctggg acctggatcc
     1861 tgacgccggt tcggcgcgca tgccgccgct gctgctgcag cccctggtcg agaacgcggt
     1921 gcggcacggc atcgagcccg cggatgacgg cggcacgata cgcgtgcgga cccgagccag
     1981 gggcgatcgc gccgtgatat ccatcgccaa cagcgtgact ggcacggcat cgcgtcccgg
     2041 ccagggcatc gcgctgcgca atgtgcagga ccgcctgcgc ctgatgcacg atgtgaatgc
     2101 gcagttcgaa agtcgccgag atggtgacgc tttccgcgtg cagatcgtgg tgcccctatg
     2161 aatgacgctg cagagaactc caccgggctg ctgcgcgtgc tcatcgtcga cgacgaagag
     2221 ctcgcccgtc tgcgactgcg agcgctgatc gatgactgtt cgcaacctcg cgcgaccgtg
     2281 gtgggcgagg tagccaatgc agcgcaggcg ctggtctggc tgcagagcca cggacaaggc
     2341 ccggcttcgc caggatccgg ccgcgcctgc gatctggtgc tgctggacgt ccagatgccc
     2401 ggccgagacg gcacccagct ggccgaggaa ctgcgcgccc ttccgctgcc gccggccgtc
     2461 gtgttcgtca cggcgcacgc cggccacgca ctgaaggcct tcgacctgga agcggtcgac
     2521 tacctgacca agccggtgcg ccgtgaacgc ctgcagctgg ccctgcagcg ggtgtcggcc
     2581 cgcctcggtc agccaaccac cgtaccgggc gaactgggct cgcagcccga cccggtgctg
     2641 atcgtcagcg agcgcggacg ggtggttcgg gtgccggtcc gcgaagttct gtacctgaag
     2701 gccgaactga aatacgtcac cttgcgaacc gccagccaca cgtatgtgct cgacgacgcg
     2761 ctgagcgacc ttgaacagcg cctcggcgag cgcttcctgc gcgtgcaccg caacgcgctg
     2821 gtagcgcgct cggcgatcag cgcgctggag cgccgaacga tgaccgacgg cgacggcgac
     2881 gccagcgacg aaggacacgg cccgaacgaa tgctgggctg tgcgagtcgc cccggtcgat
     2941 gagtggctgg ccgtctcgcg gcgccaggcc agcgcggtga gagacgcgct ggcagccagc
     3001 gggctttgat tccgggcggg acgaggaacg cgggaagagt cttcagccgg tgttgcgcaa
     3061 gcccgccgcc acgccgttga tcgagatgtg gatgccgcgc tgcatccgct cgatgcccgg
     3121 gtcgccctcg cggcgctggc ggtgacggcg catcaattcg acctggaggt gattcagcgg
     3181 atcgaggtag ggaaaccggt gctgtatcga ccgcgccagc gacgggttcg atgccagccg
     3241 gtccttgtgg ccggtaatca gcgtcagcgc atcgttggtg cgtgtccact cggcctggat
     3301 caacgcgaag atgcgcttgc cgagcttctt gtcctcgacc agttcgacgt agcgcgcagc
     3361 gatgcccagg tcgctcttgg caatcaccat gtcgagattc gaaagcagcg tctggaagaa
     3421 gggccactgc ttgtgcatgc gctgcagcag tgcgagacgc tcctggcgcg cgtcggcatc
     3481 ggtggtcagg aacttctcga tcgccgagcc gaacccgcac cagcccggca gcgcgacgcg
     3541 gcactggccc cagctgaatc cccacggaat cgcgcgaagg tcctcgatcg cgttggtggc
     3601 cttgcgggct gccgggcgcg agccgatgtt gagtccggca atctcgcgga tcggtgtcgc
     3661 gctgaagaag tattcggtga agcctggcgt ctcgtagacc agcttgcggt aggcggcata
     3721 gctggcctcg ctgatcgccg ctgcggcgtc gaggaagctc ttcggtgcgc cctgtcggct
     3781 ggccttcttg gtgtgcagca gcgtcgcctc gagcgtggcg gccaccagcg tttcgaggtt
     3841 gcgccggcca atctcggggt gcgcgtactt cgaagcaatc acctcgccct gttcggtcag
     3901 gcgtatctgt ccgttcaccg ttccgggggg ctgcgccagg atggcctgat agctcgggcc
     3961 gccgccacga cctaccgtgc caccgcgacc atggaaaagc cgcagggtga tgccgtggtc
     4021 gcggccgagt tcggcaaaca gttcgaccag ggcgatctcg gcgcgataaa gctcccagct
     4081 gctggtgaag ctgcctccgt ccttgttgct gtcgctgtag cccagcatga tgtcctgctc
     4141 ggcgaagtct cccagcttcg atcgcttgac cagtgcggtg atgcccggca ggccgtagaa
     4201 ggaacgcatg atgggctcgg cgttgcgcag gtcaccgatg gtctcgaaca gcggcacgac
     4261 gatcaggtcg gcgacggcag cgtcatcgag cgtgccgcgc atcaaaccgg tttccttcat
     4321 cagcagcagc acctcgagca gatcgctggc ctcttcggtg tgcgagatga tgcagtggcg
     4381 caaggcatcg ggcccgtagc gtcggcgcat ttcgactgcc gactcgaaga tcgccagttc
     4441 accgatcgcc agatcgctgt aggcggcggc gcgcacccgc agcggacgcg catcgtcgag
     4501 cagtcgcagc agcagcgcgc gtctggcagc ctcatcgagg gcggagtaat cgggctcgat
     4561 cttggccacc cgcatcaact cggccagcac cgcctcgtgc ttgtccgagc tctgacgcag
     4621 gtcgacggtg gcaaggtgaa atccgaacac ctgcaccgcc cgcatcagcg ggcgcagacg
     4681 cggcgcggcc agcgcctgtg catggtgcgc attgagcgat tcctcgatca cccgcaggtc
     4741 ggccaggaaa tcactcgccg acaggtaggc gttctgcggc ggcaccgcat ggcgcaacgc
     4801 ctcggtgccg gtcaggtcct gcaacgtggc cgccagacga gcgtagatgc cgaccagcgc
     4861 acggcggtac ggctcgtcgt cgcggtgcgg gttgtggtcg ggtgacgatt ccgacagttc
     4921 attcattgcc gcagtgaccg gcgccagcgt cgccgagatc gacagctcac cgccgagctg
     4981 gtgcacttcg gtcagataga agcgcagcac cgtctcgctc tgccgcgcca acgcaacctg
     5041 caaggtgctg gcggtgacga acggattacc gtcgcgatcg ccgccaatcc agtttcccat
     5101 gcggaagaag ctcgcgatcg ggtggccggg cagcgcggct tcgatgtcgc ggtacatgcg
     5161 cgggatctgc cgcaggaagg tcgagtggta gtagctcagc gcgttctcga tctcgtcggc
     5221 cacggtcagc ttggtattgc gcagcatccg cgtctgccag agctgggtga cgcgggcgcg
     5281 gatcagcgcc tcgttgtcgg cgcgatcccg cgcggtgtgg atgtcgtcgc ggtgctcgac
     5341 cagttcagcg atggcacgtt cggcgtcgag gatgctcttg cgctgcacct cggtcgggtg
     5401 cgcggtcagc accggcgaga tgtaggcatg gtccagcgtg cgcacgatgt cggcggcgcg
     5461 gacgtcggcg gcggccagcc gctcgaatgt catcgcgagc gagccttcgc gcaactcgcc
     5521 cttctgctcg tgcaactcgc gcaaccgcac gttgtggcgg tcttcggcaa tgttggccag
     5581 gtgcgagaag tagctgaacg cgcggatcac gctgaccgtc tggtctcccg acaggttctt
     5641 cagcagccgg tcgagcgcac ggccggcctg cgcatcgcgc ttgagccggt agccgaccga
     5701 cagctgccgc acgcgctcga tcaattcgaa cgcatccttg ccttcctgct cgcggatgac
     5761 gtcgccaaga atgcggccga gcagccggat gtcttcgacc agcgggcggt tcttgtcggc
     5821 agcgtcagcg tggggcgttt tcttcggcat ggtggatgga cttcgcggtc gggtttcgaa
     5881 atggggcgcg cagggcgcga gccggacctg aattggggga gtccagaagg cgttgcaaga
     5941 ctcgcgcctg ctagcatctt cctatgacct cacacatcat catcgccacc cgggagagcc
     6001 gcctcgcgct gtggcaggca gaacacgttc gcgacctgct gggcagccgt ttcggactcc
     6061 gcgtcgaatt gctcggcatg accacacgcg gcgacgagat cctcgaccga acgctgtcca
     6121 aggtcggagg caagggcctg ttcgtcaagg aactcgaaac cgcgctcgag gaaggccggg
     6181 cgcaactcgc agtgcattcg ctcaaggacg tcccgatgga ccttcccgac ggcttcgaac
     6241 tggctgcggt gctcgagcgc gaagaccctc gcgatgcctt cgtctccaac acccacgcct
     6301 cgctggccga tctgccccat ggcgcgaagg taggcacatc gagcctgaga cgcgtcgtgc
     6361 aactgcgtgc cttgcgcccc gacctgctgg tcgaacccct gcgcggcaac ctcgacaccc
     6421 gcttgcgcaa gctcgacgag ggccagtacg acgcgatcgt gctggcggcc gcgggcctga
     6481 agcgcctggg cctggcctct cgcatccgca gcgagttcga taccgaccag atgctgccct
     6541 gcgcaggtca gggtgcactc ggcctcgagg tacgcagcga tgcagccgcg ctgcggacgc
     6601 agctcgccac gctcgcgcac gcaccgaccg cgctggcagt gcatgccgag cgggccgtgt
     6661 cgcgcgcact cggaggcagc tgcagcatgc cgctggcggc gcacgcggtg tgggccggga
     6721 gcgcgctcag cctgcgtgct gcggtcggtc accccgagaa ccccgacgca ccgctgctgc
     6781 gcgccgctgt cagcgcagag gtgcagaccg aggagcaggc ccgcgcgctg ggcgagcaag
     6841 ccgcggccgt tctgcgtgac tcgggtgcca cgctgtatct ggctgcggtg gccacccact
     6901 gatcgagccg tcagcggcac gtgcccatca ctgccgatcc agacgctcca gagccgatcc
     6961 atgcgcgtca tcgtcacccg ccctgcggca caagcgatgc cctgggtcag gtcgctcgag
     7021 aagcatggcc tcaaggcagt ggccttgccg ctgatcgaca tcggaccggc tctcgacacg
     7081 cgatcggtga ccgaagcctg gcaacagctg ggccaagcgc gcctggcgat gttcgtcagc
     7141 gccaatgccg tggagcattt ttttgcgctt cgaccgatcg acaccgtctg gccgacgaac
     7201 gtgcaggccg gctctcccgg tccgggaaca acgggcgcac tgctggatgc tggggtgccg
     7261 tcgtcactga tcgtcgagcc ggcagcggac agcgcacagt tcgactccga gtcgctgtgg
     7321 gagcgcctgc gtgaccagga ttggcgcgcc gcaccggtgc tcgtggtccg cggcgatggc
     7381 gggcgagact ggttggccga caccttgcga gcgcacggcg ccgaggtgag tttcgtttca
     7441 gcgtaccgtc gaaccgcacc gacttggaat gacaagatgc aaacactgct cggcgaggcc
     7501 ctgacccgac ccgacgagca tctctggtac ttcagcagtt ccgaatcgat cgaccacctg
     7561 atggaccacc tggcgaattc ccggcagcag ccgaccccga gcacaccgat cggactgccc
     7621 atcggcgccc gcgcgctggc cacgcacccg agaatcgccc gccgcgccga gcaggccggt
     7681 ttcgcgccgg tctggctgtg cccgccgacg ctcgccgacg tggtcgcctg catacaatcc
     7741 gccgcaccgt gaccgaccac cctaccgcag cagttccagc ggagccaacc accccgttgc
     7801 ccgtggccga agcaccatct ccagcgggcg cgtccgtgcg cccggagacc tctttcggcg
     7861 ccatgcgctg gtggtctgcg ctggccgcgg tgctggccgt catggccgga gtcagcctgg
     7921 tgatggcctg gaagacccag caacgcgtca agtccgtgga gcagcagctc gtgcgccggc
     7981 tcgaggacag ccagttgcag gccagcgaag cgcgcttgct ggccaagcag tcgcaggaca
     8041 gcgtgctggc cgcggcggcc aaggtcgcgc tgctcgaagc ccgggtcgct gaagtggcgg
     8101 tgcaacgcag ccagctggaa gatctgatgc agtcgctgtc gcgctcccgc gacgagaaca
     8161 tgctcgtcga catcgatgcc ggcatccgtg tggcgatgca gcagaccgcc atcaccggca
     8221 gcgccgagcc cctggtcgcg atgctgaagc aggcggacga acggctggct cggtatgacc
     8281 agccgcggct cgaaggcgtg cggcgcgcca ttgcccgcga tctggaccgc gtcaaggccg
     8341 tgggcgtgat cgacgttccg acgctcagca tcaagatcga cgaggtcgtt cggctgatcg
     8401 acgaattgcc cctgctttcc aacgccgaac agcgcccggc ctcacccagg gcaaaggcga
     8461 ttgctgctgc agcgtcctct gccgttccgg tggtcgccgc atcgggttcg gcggcgggcg
     8521 tccgcgcggc atcgtcgccg tcaacggcct ggtggcagac ctgggacggc agctgggccg
     8581 cgatctccga gcgggtgtgg agcgaggtga aatcactggt cagggtgact cgcatcgacc
     8641 atcccgagtc gatgctgctg tcaccggaca acgccttctt cgtgcgcgag aacgtgaagt
     8701 tgcgcctgtt gaacgcccgg ctggccctgc tgagtcgcca gttcgacaca gcacaaagcg
     8761 acctgcaggt ggtgcaggcc tcgctcgacc gctacttcga ccgcagttca cgacgcactg
     8821 cgctcgcgac cgacctgttg cgtcaggtca ccacgcaggc ccggcaggtc acggtgccca
     8881 agccggacga caccctggtc gcgctggctg ccgcgactgc cggtcgctga caggcgacga
     8941 gcagcgatgc gctccgtcat ctggttcgtg ttgctgttcg gcgctgccgt cgtggccgct
     9001 tcgaccctgg gcgccaacga tggcctggtc agcgtctacg cgggtacctg gcgcttcgac
     9061 atatcgctga acctcttcct gctgctgctg gtgggcagct gcttcgccct ggtgtcgctc
     9121 atccacgccg cgaacagtct gatcggcctg cccgagcggg cgcggctgtg gcgagtcgcc
     9181 cgccgggaac gcacggcgca ggcggcactt cgggaagcgc tcgcgcagta tttcagcggc
     9241 cgctacagcc gtgccaaggc cgccgcacag caggccctga caatccagga cgacaccccc
     9301 gaactgaagc cggacaccga gttcagcgtg ctcggcctgc tgctggccgc tggcagcgcg
     9361 caccgtctgc aggatcgctc caagcgcgac gagcacctgg ctcgcgcatt caagttggcg
     9421 caaggcagca cggcggcgcg ctcggcagaa gagggtgcgc gcctgatggc ggccgaatgg
     9481 gccatcgaag accgcgatgc acctcgcagc ctgatcctgc tttccgagct gccgccaggc
     9541 gtagcgcgtc gcacccaggc tctgcgcctg aagctgcagg ccgcgcggct cggacagcag
     9601 ccccaggagg ccctgaagac cgctcgtctg ctggtcaagc accagggcct gtctaaagcc
     9661 gccagccagg ggctgttgcg ttcgctggcc ttcgaatgtc tcgataccgc gcacgacatc
     9721 gaccagctca agcggatctg gctgtcgctt gacgctgccg atcgccgcga caacttcgtg
     9781 gccgcgaggg cagcggtgcg tgcagcgcac ctgggctcga ccgatgacgc ccgcagttgg
     9841 ctgcgccccg cctgggaccg catcgaggaa acctcggacg acgaacgtgc agcgctctcg
     9901 cacgcactgg tttcggctgt tgccgggatc ggcatcgact ggctgcagcg actcgaatcg
     9961 gcgcggctgg ccttcccaag ggacgccgca gtggcgcatg cggtcggcac cgcgctggcc
    10021 gagcgccagc tctggggcaa ggcccgccag ctgctcgaaa cagcggctgc cgatgccgat
    10081 ctgccgatca ccgccaagcg catggcctgg ctcacgctgg cgcggctggc cgaaaacgag
    10141 ggtcagccgg aacgcgccgc catcagctat cgaaacgcag cattgctgag gtgaaaaggc
    10201 cgcgggtgct gctatactcg tgggcgttgc ggctgtagct cagctggata gagtacttgg
    10261 ctacgaacca aggggtcgtg ggttcgattc ctgccagccg caccagtcaa atcaaagggt
    10321 tagctctcac aaggagctag cccttttcgc tttctgggcc gcatgcgtcc cgttgccccc
    10381 tattgccccc acaccgcttc gtgctcgcgt tggcgactcg ctaaacccgc gagtgagtgg
    10441 tcagcatagg tcaggacaat gccaccggga atcgggctat ctcttcgatc ttctgagatt
    10501 ggacgtcgag aatctgtctg gggtagcttt atttgaggcc gctggaacag cccgcgtgac
    10561 aggatccaac ggaacgagcc aagactttgc cggcgatgtc ttgggcacct tgtcgctgag
    10621 cggattcgca aacatctctt ggttcgaaat tcgatcgcta gacggtctag caaacgatcg
    10681 acaacatcac tctccaacca gcggttgctg cagtgcccga gcccaccgcc ctggcgctcg
    10741 ccggcgcagc catgccaggc atcgccgcca gccgcagacg caagccggcg tcatctactg
    10801 cctgacggtc tgccaactcg gaggttgacg gcgcctgcca tcggacagcc gtgcgcggcg
    10861 cttgctgatg gcgcgccgcc cgaggcccac ccgggtgacg tcagccgcgc cgccgcacca
    10921 cgtccggcag gaagcgaacg gacagcacca gcagccccag cagcgccggc acccagcgca
    10981 ggtcggtcgc cacgggctgc ggccgggcca gcgccgggtc gagcatcgcg tcggccagca
    11041 catcgggcgc agtcaagtgc cggtagccga gccccaggag cttgcccagt gcctgcaggt
    11101 gcggttcgcg cagttccgac aggtgctcgt gcgcggtccc gggcgtgccg gggccgaagc
    11161 gctgcaccac gtcgcccgcg gcccagtagc ccagcgggcg gccctcgccg tcgaagcgag
    11221 ggatgcgcgc cggcacgtcg ccacccacac cgatcagcca gccgcccgcc tcaccgggcg
    11281 tgatgtcctg cattggtggc ttgacctcct ggctgcgcaa gggcggagac tcctggccgt
    11341 cggtgaagaa gacgacccgc gtgtccgggc cgatggcctt ggccgtgcgc accagccagg
    11401 tcgcgccctt gccgacgttg ctggcgttgg cccagcgcat gcggccgtct atgcgctcca
    11461 gcgaggccag cagcgcatcg tagttgccgc acacctcgat cggcagcacg agcggcatca
    11521 cgcggtagtc ggcgaagatc gcccagccca ccctggaacc gcagggcagg cggcgcagcg
    11581 cctcgcgcgc ggccgcgcgg gcgaaggcca gccgcgtgac cgcggcgccg tcgagcgcct
    11641 ggtcgtcgac ctccatgctc tgcgtgacgt cgaaggcgag caggtactgg taggtggcgc
    11701 gctgcagcat gaccggcggc atccacaccg cggcagccag cagcgctgcg gccgcgacga
    11761 tgggcaggtt ctggcgtgtg gcgaggccgt tcagcaggct gcgccatgcc ttcatggcag
    11821 gtctccggcg atcatgccgc gcaacgcgac gttacggcgc tcgaccggca cgttgggcgc
    11881 ttcggcggtg gtctcctgtt cctcgggtgc ggcgcgcagc gtgcgctcga ggttgtagcg
    11941 ggcgtcccag tcctgcgggt cggcgcgcag caggtcgcgg tagcgctgct tggccagttc
    12001 gagcagtggg ccggtgtcgg ccggcgtgct gctggccagg ccctggcgca cgtacatgtt
    12061 gcccaggttg tactgtgcgt gccggctgat ggcatcgctg cggcctggct cgatcagccc
    12121 ggcaaagtgc ttgaacgcgg cgtcgaaggc ccccgcctgc gaccaggccg tggcctgggc
    12181 caggcgaacc tcgcgcggtg cgtcccggga gaccgtggat tcggcagcgg aggctgacgg
    12241 cgccagggcc tgcgctgcgg ccgcgatggt ggtgttgagc tgccgcgcct gctgccatcg
    12301 caggccgttg aagccggccc cggccagcgc cgccgtggtg gccagcgcaa acgccgcgtg
    12361 catcgaaatt cgtctcatgt caggctcctc caggtttgga gctgcagcgc gcggcaggtc
    12421 agcagcccca ggcagcaaag cagcgccgcc ccgaagcagc cggcgctgaa atccagccgc
    12481 ggcacctgct ggatgaaggt gaggggaaag ttctgctggc ggtcgatctc ggcgatggct
    12541 gtggccatgg cctccgggtc gtcggtctgg tacaggcggt agggcgtggc cagcgtcttg
    12601 aagaacgcgt gcagcgcacc ttcctcctcg gagtccatcg tgcccgcgtc ggtgcgggtc
    12661 aggtcgggcg aattggggcc gctgcggatg tagatccagt acagcccgat gcggtggcgc
    12721 gccaggccgg tggcgatgcg ctggcgctgc gtgtcgtcga gctgcgcgcc gccgtcggac
    12781 accacgatga cgatgcggct gcccgaatac ggacgccctt cgaactgatc gatcgctgcc
    12841 agcagcgccc tgcccatcag cgtgtcgggc aggccgcggc cgatggcggt ggcctgcagc
    12901 ccggcctgca cggtggcgtg gtcctgcgtg aacggggcca ccagcatcgg gctggtgctg
    12961 aaggtcatca gcgcgaagcg gtcatcggga cggcgcttga cgaaatcggc cagcagttcg
    13021 cgcaccacct ggatcttggt aggcatgctg gcgttgaact ggccgccggc cttgacgtcc
    13081 ttgggcagca ggttggcgtc catgctggcg ctgcggtcca tcaggatcag cacctcggcg
    13141 cccttgccgg tgcgctgcac ctgggtaccg ctctggccgg gaccagccag gccgatcacc
    13201 gcgccggcca tcgcgccgca ggcgaaggcg cgccaaagca agccggcggc ccggccccac
    13261 gggtcggagg gcagccagcc gacggacgag aagcgcagcg tgtcgcggcg ccgccgcagc
    13321 agcggcagcg cagccagtgg catcagcagc agccacagcg gttgcaggaa gtccaagccc
    13381 atggtcagcg gtggtggcgg cgctcggcct cgcgcagtgc caggcccagc gcgcgcagcg
    13441 acaggtcggc cggcgccgtg cccgcctcgc tggcaaagaa gcgctgctcg gagtggcgga
    13501 agaaggcctc cagctgcggc gccagcggct gcaggtgggg agcatcggcc agcagacgcg
    13561 gcagcgtgcg gccgtgcacc acgcgtccgg cggtggcgtc cagcgcacgg tgcagcgccc
    13621 gccaggcctc ggtgctggcc gggtcggctt ggcgcagctg ctgccaggcg cgggcaaagg
    13681 gcaggcgccg ggcttcgcgt gcattgcgcg ccgcccacca cgcggcccag cccagcagca
    13741 ccacggccag cgccgtgagc gagagcttga actgccgctc caggccttcg gtgggcaccg
    13801 gcagcaccgg gcggtcgggc cgcagcggct gcagcgcgcc ctggtcgaag gtggccggcg
    13861 gcgtgagcgg gccgatgctg accggccagg cgaccacctg cagcgggccg gccgaggtgg
    13921 tgatggacag cgcaggcagc gcagcggccg tcagcgcacg cggtgcgttg acgacctggt
    13981 agtcgatggc cagccagcgc cggccgagcg cgtcttcctc gacgcgcggc gtgcgccgtt
    14041 ccaggtacag gcccacgcgg tcggccgccg gcagcgcgcc ggcatcgacc gggcggccgg
    14101 cgtgcgacag cagcacgcgt tgcgtgagca cgtcgcccag cacatggccg aagcttcgcg
    14161 gctgctcgac ggtggccgcg ggggcagcag gcgtgggtgg tgactcggca gccaccgacc
    14221 gcatcagggc cagcagtgcg gccgccagca gggtgcggtg caggttcacg ccacagcctc
    14281 catgaagtgt cgcgtcaggg cttccgcatc gaagcagccg tgcaggtgaa agggcaccag
    14341 gccatgggcg tcgaacagcg cacgcagcgc ctcgcgccgt gtcgccaccg cgtcggccca
    14401 gcgctgtcgc agcggctcgc gcatccacag tgtgcggcgc acgcccgtct cggcgtcgct
    14461 caccgccacc agcgcgtgtg cggccggcgg ctcggtctcg gccgggtccc acacgaccag
    14521 cggcaccacg caggccgggg ccagcaactc cagcacgccg tgcagcggcg ccagcggcca
    14581 gtggaagtcg gacaccagga acaccagccc gggccgcccc gccagcgggg ccaccgtgcg
    14641 ctgcagcgcc tgggccgtga gttcgtgggt gcgatcgtgc tggtaggccg gctcggcctc
    14701 gcagcggcgc agcgcgtcgg ccatcacgat gccggcgccg cggctgtggc gggcgggcat
    14761 gaagaggtcg tcgcgttggg cgtggtcgaa ggccagcagc cccagcgcat cgccgctgcg
    14821 aaacgcgctg tgacccagcg cctcgacgaa atcggccacc acctcgagct tgctgcgcag
    14881 ggcgccgaag tgcatggacg ccgacacgtc gaccaccgca tgcaccggca ccgccacgcg
    14941 ctggcgctgg atgcgcacca gccagtcgcc gcgcacgtcg cgcaggctgg cgcgcaagtc
    15001 gatgcggcgc gggtcagggt ggtccagcag ccgccgatgg gccgcgaagg cctggccggc
    15061 gcccaggctg ctgccgcggt gcgcgcccgg ccgctgcccg cccaaggtgg cgggcaaccg
    15121 gtagtggaac tcctgcggcg ccaggttcgc cgccatcacg gcgcggccac ctggtcgatg
    15181 atcttctgca ccagcgccgg ggccagctcg gcgcgccgca gctcatagac cggtgtgaag
    15241 aacacgcggt gcgccagcac cgacggcagc accgccagca cgtcgtcggg caacaggtgc
    15301 gtgcggcctt gcagccaggc caccacgcgc gccgcgcgca ggagcgcgct catgccgcgc
    15361 gggctggcgc cggccagcac caggcggcgc atgtccactc cgtcaagctg cacgccgaag
    15421 cgcagcgggt cttcgctggc ttcccacagg tcgagtacgt agctctcgat ggcctcgctg
    15481 ctgctcaccg agcgctggat gagcgcgccg atggcgttga gctggtcagc gcgcaccatc
    15541 gccgcgggta cctgctcgat cagctggtcg gcgtcatgga aggcggtgtc gaagaccagc
    15601 gcgcggcgga tgccgcggtc cacgggcttg ctcatgttca gctcgaacat gaagcggtcg
    15661 cgcgcggcgg aggccagctc gaaggtctct tccttctcca ccttgttgcg gtcggcgaag
    15721 accgtcatgt gcgggaagcg gtactcgcgg ttgaaggccg agaccgtgcg ctcggccatc
    15781 gcgcgcagca gcagcgactg cacctgcggc cgggcgcggt tgatctcgtt gaagaagaag
    15841 gtggtcagtc gttcgccgtg gcgcagcagc gggccgggat cgatgcgcgg ccggccctcg
    15901 gcgtcaacgt aggtgtggta gatcaggtcg ccgggcatca ggtcgaccgt gccttcgacg
    15961 cgttcgaagt cgccgcccac cgcccgcgag aaggcccgca gcacggtggt cttgcccacc
    16021 ccgacgtcgc cttccagcag cacgtggccg cgcgcgaagg tggccacgtt gatcaggcgg
    16081 atggtggcgg cctggccgat cacggccttg gcgacctcgc gctccaggca cagggcctgt
    16141 tcacgccagt cttgcagttc tcgttctggg ttcatgctgc gggtgcggtg atgaaaatgg
    16201 ggggcaggcc accgcgccgc gcgcccggcg ggcggcggcc gaaggggtga cggggcaagg
    16261 gacgtcagcg cgcggccggc gggtcagtca tgcgtgcccc cggtggtggg cacggggaaa
    16321 ggcagggcgg ccgtcacgtc gtccagacgc accaggccga cgctgagcgc gaaccacacc
    16381 agttgcgcat cggtgcgcac gccgatcttg ctcttgatcg cgtagtggta gttgcgcacc
    16441 gtcttctcgc tgaggttcag tgcatcggcg atggcatcgc tggtgtggcc gctggccacc
    16501 aggcgcagga tctcgacctc ccgcgcacca aggtcgtcca gcaccgcaat cgggtcggcc
    16561 aggcgctgcg tggcgatggc ctggcgcgtg tcgtcgcaca gcgcctgctc gccctgagcg
    16621 acggcagtga cggcgcgcac cagctcgttc ggaccgctgc tcttggtgac gtagccccag
    16681 gcgccggctt ccagcacctt cagcgccatc gaggcaccgc tgtgcatggt gaccaccagc
    16741 acgcgcgcat cggcatcgcg ctggcggatg tggcgcaacg cgtccagccc gctggcgccg
    16801 ggcagcagca tgtccatcac caccacgtcc gggtggtggc gacagtaggc ctcgtaagcg
    16861 ctgtcggcgt ccaccgcttc ggcgcagacg gtgaagccgg gctggcgttc gagcaggcgg
    16921 cggtagcctt cgcgcaccac cgggtggtcg tcgaccagca ggatggacac cggcttcaag
    16981 cggcggcccg gcgtgcgctg gggtcgttgg cggccagcgg gatgatggcc gccacgcgga
    17041 tgccgccgct ggcgttgtgg atggccagcg tgccgcccag cgcggcgagc cgctcgcgca
    17101 ggccggtgat gccgtggccg ccgctggaag cctggccgat gcgggccgca tcgccgccgc
    17161 cgtcgtcctg taccgtgaca gccacctcac ggccttcctg ggccacgcgc agcgccacct
    17221 gcgtgggcga gccgtggcgc gcggcgttgt tcaggcactc ctgcgcgatg cggtagacgt
    17281 cgatagcgat cggccgtggc agcgcggcga tctcaccgtc cacgctgagc tggaacaccg
    17341 agcgcgaggt ggtctgcagg ttgtgctcgc tcaccagctg gcgcaggctg gcctcgaggc
    17401 cgatctcctc gatgtcctgc gtgcgcagcc gaaccagggt gctgcgcagc gtcttcatca
    17461 tgcgggcctg cgtgcgaccg atggcctgcg cttcctcgac gatgtccggt cgcccggccg
    17521 gcgcgccggc ctcgatggac gcggcgagcg cagcggtcgc ggtgaggcac tggccgaact
    17581 cgtcgtgcag gtcacgcgcc agcgcacggc gttcgttttc ctgcacctgc agcagccggg
    17641 tggtgagcgc gttgcgatcc tcgttcgtgc gcgccaggcg gccggcgagc tggttcaacg
    17701 cgctggcgat gcgcgcgaac tctgcggtgc ggaacgacgg cagccgccac gccaggtcgc
    17761 cctgttccag ccgttgcaga ccgtcgacga tggcgcgtgc cgggcgcagc gtgcggccga
    17821 tcatcagcgc cgccagcagt gcgatggcga ccgccatgcc ggcggcgatg cccgccaccc
    17881 acgacacctg gcgccaggcc tggcggatgg ccgcgccccg gtcgggcgtg ttgacgatga
    17941 agccggagcc agggtcgcgc gcgaaaagcg gccgctccac agaggtgtcg tgcgggaaca
    18001 gcgtgccgta ggcgctgacg aaccagtccg gcgcgcgcgg cccgagcgag gccgtctggc
    18061 tgcacagcgt ctgctgcgca tcgccacgca gcgcaaacgt cacgcacacg ccgggcgaga
    18121 tgaggttcag cgtggcgaac gactgccact ccggcgccgg cgtgttgagc ccggcccgcc
    18181 cgcgcccgcg ccacagctgt tcctgccagt aaagcgcttc caggtgctgg ccgacgcggg
    18241 cggcggacaa ctcggcctcg cgttccaccg agcggtgtgc atcgaacacg atccaggcta
    18301 tggccgccgc cagacacaat gcagtgacca cgaacaggcg ggtgacgagt tggggcagca
    18361 gttgcatcgc ggggcgatcc gttcgtgtga ggtccagccc cagtgtcagg cagctcggcc
    18421 aggccccgca agacgcgcaa acaccgtacg gcgtgcggtc aggcaaattg cccaggcgcc
    18481 cgcgagctgg ggccgacggg cgcatgccgg gcgacctgcg gcggaaccag ttcccgcggg
    18541 tgcacctggc tctgggtcag cgtgccgcgg ttcgtatcgg tgcgtccgcg aagcacaacc
    18601 gcatggagac cggatgcgtc aggtgctggc cagcttgagc ccggcgaggc ccaggctcgc
    18661 cacgcgacct gcgctggcgg gctagccgaa cagcgccatg ctcaggaccc tggcggtcag
    18721 cgtgaaaaca ctgggccaca cccgggtgaa tcccaacgtt tacttaagac cgatggccca
    18781 gccgacctca agcaggccgg cgacgacctg ctacgcctat ggcatgcgga tggccgttct
    18841 tctttgatcg gcgtccgcca acggatgcga gatgccgcca tcgccacccc tcttcagcac
    18901 ctgcacgatg gtgacgtgat cgccctcagg ccacaggccc tggagtttcc cgccggcatg
    18961 ctgcccggga tgttcgacgg gttgcctcag cgcttcgtgc gaatccccgt agtacaccgt
    19021 cgagatcagt cccgaagcag tgttctccca ggcctcctcg ccgaggcagc ccgggatcga
    19081 ctgggcgatc tccacgatgg cctggtctcg ctggtgaagt tcgtagtcgt ccccgcggac
    19141 gaacttctcg ttgtcgcgca cgtggatcgc gccgtgcgcg tgcaccactt cgcagtttca
    19201 ggtcggcacg cgacggtgcg tttcgtgcct gccggggtac cagttcggcg tgatgtagcc
    19261 ctgcatgcca cggaagacca cgagtacacc gtcgctttcc ttgatctcgt caccagccca
    19321 gagccatcac tcgtggggtg acaggccgcc cgacacccgg ggcaagtcgt tgggctgggc
    19381 cgcggcacct gcaccgctct cgagcaacca gccgacgccg cacaggcctg tcatcaacag
    19441 cgcggtccac aggatgcgtg cagacatgtc catcgcctcg gcatcccaca ggaagaagcc
    19501 gcaaccgacg gcagtcgtca ggaacagcac caccgcggcc gcacgcgccg cagcactggc
    19561 gggggggtcg tactgctcgc ggtgcaggtc gaagggtggc tgcgggaaac gctgcgcgac
    19621 atcgtcagga cgccagccgg gcggcatgag cagcacccgc agcttgtcct gccagcgctg
    19681 cgtgtaccag gcgtcgcgcg ccagcgcgcc atagacctgg gtgatcgccc gcagcggatt
    19741 gaagctgcgc accggggagc gcgtgccgaa cacgcaaggc tcggtttcgg ccgcataggt
    19801 tccgaacagc cggtcccaga gaatgaagag cccgccatag tttcggtcga gatagcagtc
    19861 gttcaccgca tggtgcacgc ggtggttgga gggggtggtc agcacaccgt ccagccaggc
    19921 gatctttccg atctgctcgg tgtgcgccca gatctggtag tactggacac cgagcagcac
    19981 gaaaccaaac atcaccggcg gcacgcctgc gatggccagc ggcaggtaga acggccagcc
    20041 gagcaaagcg ttctggcttt cctgccgcag ggccgtgcag atgttgagct cctcgctctg
    20101 atgatgcacg acatgcgcgg cccagaagat ggcgacctcg tgcccgaccc ggtgcaacca
    20161 gtactcgcag aaatcgaaca tcacgacagc cagcgcgatg ccgtaccagc tgtgccagaa
    20221 cggcgattcg acctgatgtc ccagggccga gaacaccatc gcgtagatgc cgatctgcag
    20281 cagcggcgtg cacacgccga ccagttggct gatcagcccc tggctcaggt tggcaagggt
    20341 gtcgttggtg cggtaggact tgcgctttct cacgaaactg accgtcatct cgagaatgac
    20401 ggccagggcc aggaaaggaa tcgtccagat gacagcttgt tccatcgtct cttcaaggca
    20461 ggtggcaggc gaatttcctc agcgccgcac ctacggagcc cggcggggct ggcgaccatg
    20521 atcccggaga ctcgttccgg tagatcacgt caccccatcc cgtgaagtcc gtgtgtcggc
    20581 tgaagaacag cgaacggctc tgattttcca cgcagtcgaa ttcgagttgc tgcctcgaag
    20641 acgagtaggc cttgccgtcc ggcgcgatgc gaggctgggc gtagttgatc acgctccaca
    20701 tcttcaccat gtcgccactg cgcacgatgg tgctggggtc ggcgagcacc gtcaccacgc
    20761 cgtcgccgcg cacgcgcacc cagttggccg ccgccgatcc ggcagcacag atcagcaaca
    20821 agcacacgga ccatttcttc atctgcacac tcgaatcgat gcgtcccggg aacgtcacgc
    20881 ctcggatgcg gcgttatcga tgcgttggcc ccgatccagc cgaactagga ctgcaaccga
    20941 tcgagcgcat cttcgaggtt cttgaccgtg cgcgcggggt tgtgcagctt gtccagtccg
    21001 aacagaccga tgcggaaaga ctggaaatcg gggccttcgt cgcattgcag gggcacgccg
    21061 gcggcgatct gcaggccgag cccgagccac ttcttgcccg actgcatgtc ggcatcctcg
    21121 gtgtagctga ccaccacgcc aggcgcctgg aagcccggtg ccgcgacact cttgaagcca
    21181 cgacgcgtca gcatctcgcg cacggcattg ccaagctgct gctgctcgtc gcgcaccttg
    21241 tcgaagccgt aatcgcgagt ttccagcatg acgtcgcgca cgcgcgccag tgcatcggtc
    21301 ggcatcgttg cgtggtacgc atgtgcaccg ccttcgtagg cttccatgat ctgcatccac
    21361 ttgcgcagat cgcacgcgta gctggtgctc gtggtgctgt cgatgcgcgt gcgggccagt
    21421 tcactcatca tgaccagcgc gctgcagggc gagccgctcc agcccttctg cggagcgctg
    21481 acgagcacgt cgacgccggt cgccgccatg tcgacccaga tcgtgcccga ggcaatgcag
    21541 tcgaggacga acaggccgcc aacggagtgc acggcatcgg ccacggcacg caggtagtcg
    21601 tcgggaagga tcatgcccgc cgaggtctcg acgtgcggcg caaagaccac gtcgggacgc
    21661 agttccttga tggtcgcgac gacctcgtcg atcggcgcgg gctcgaacgg cgcctgcttg
    21721 ccgggaccgg tacgccgcgc cttcagcacg gtcgactggg cggggatgtt ccccatctcg
    21781 aagatctgcg tccagcggta gctgaaccag ccgttgcgta tcaccagcgc cttgcggccg
    21841 gtggcgaact ggcgagccac cgcctccatg ccgaaagtgc cgctgccggg cacaacgacc
    21901 gccgatttcg cgtgatagac ctccttcagc gtcgcggaaa tgtccttcat cacctgctga
    21961 aagcgcttcg acatgtggtt gatcgcgcgg tcggtgtaga ccaccgagta ttcgagcagg
    22021 ccgtcgggat cgacgttggg taacaagcca ggcatggtga gctccggtga aaagaatgcg
    22081 cctcgaagac gaggtccggg gatcacacgg cggccggcgc atgccgcgag aagaggcagg
    22141 tgccgcaggc atagcgccga tctggagagc cagtcgacgc gcccgtgtgg gcttcgtatt
    22201 ttgacctgag gcgccaggcc ttgcccgtga cgggataccc gaatagtcag gatcagcgga
    22261 agtccgccag atccatctgg atgcgtccgc cgctgatgaa cgtggccttc ccgggctcgt
    22321 cgcgctccag acgcaccagt ccgactccgg ggcagtacca ctccagtgtg gtcagcggaa
    22381 tgtcgcgcca ccccaccacc gggtccgcga agagtctgat cagcgcctgc cccttcacgc
    22441 gcaggcagcc atcgaacttg cccgccgagg tgtccacctt ctggttcact gcctcgatga
    22501 cgtagttcat cgggatgacc ggatgcttgt agcggacctc gcgcgggaag tcctgcttgt
    22561 gctccagcag gtaggccgtg gtggcgctct gccatgacgt gccgacggca tacggccgct
    22621 tcagcacata gcgccgaacc gggtctggcc tgggctcgtc ctcgatgtcg cttctgctgg
    22681 caacccggta cacgccggtg tcgtcgctgc gcagccagta attggcaccg ctgtcgctgc
    22741 gtcggaccca tgcaggctgg ccgtcgaaat cgacgcggcc gagcgagctc agcgtcatct
    22801 cctcgcgctg gacggtgttg ttctccagtt cggtggccac cttgtaggcc cagcggtggc
    22861 ccttgtcgag cgggaagagc gaggtgtctc cgcccggccc gccgcacccg accaacgtca
    22921 agcagacaac gccacccagc aaggcggcac ccgttttgat ggaggaagcc atcgaactca
    22981 tttctccggc aactgcggca ccggcagatc gcggatcttc accacctgct cgtcgttacc
    23041 cgggcgcagc caggacgtac cgcccttgcc taccttcggc tcgctggtgc ccatctggct
    23101 gacaccctgg cggaccttct tgatgccctc gttgagcatc gagtgcacgt ccttggagta
    23161 aggaaggcgg taggcgcgcg gctgcgcggt cggcttgccg ttgtcgatgg cgttgaccca
    23221 cacgtagagc gcaccgtcgg tcctggcgct gggctcctcg atcacggcgg caagcagcac
    23281 gaagcgctcg ggcaacgcgt cggggctggg ccagcccgac agctgatgca ccacgctgtc
    23341 ggcgtagaaa tagaacaccg tgacaccgac cacaaggatt cccttgagcc aggcgggcca
    23401 tcgcgaccac agcagcgcca ggacgcacag cagcgccaac gcggcaaaac tcagaatcaa
    23461 cgcgagggtc atgggttgct caccagtttc ttcggcaggg tgttgacgtt ggtgacctgc
    23521 ccgtcgggca gcaccgtgaa tcgcacggcg gtggcctcgt cgcccttgcg cgccagctgg
    23581 acctgtccgt agtagacgac atcggcgcgc gggttgacct tgatcacact gatcgtggcc
    23641 tcgacagctt caccgttctt ggtctcgtag tactgcaggt tgatggtgaa ttcgcccggc
    23701 tcgatgccgc ggatcgtcac cacctcctga ttcaacgggt tcacgatctc cttgccgttg
    23761 atcaagatca cgtcgccgga caggccccgg tcatcgcgat ccaggtgcat cagaccggcc
    23821 tcgcgcgcac ggaaccagac caggttgccg cccggatcct gcacccaggt gtcgatgtcg
    23881 ttcggattgc cgtcgggcca gctgacattg acgatgtatt cggccttcga tgggatgtca
    23941 ccagccttca gcgccttcgg gttcatcgcc agcagcgcaa tgatgaaaca gaagacgaac
    24001 gcgatgagca tgttgaacag catgtcgtag aaggggtcga cctctgcttc acgcgatagc
    24061 cgtgcggaag gtggcttgcg catggctcag cccgtgaagc tcacgaccgc ttcgagcgtg
    24121 cgcgggggca cgtcggcctc gacgctcagt ccgaagcgct ggcagtccgc cacgaatgca
    24181 tcggcatagc ggtcgagcct cgtcagctgc aacccgagca cgatgttgcc gaccagtccc
    24241 accatcgtgg tcagcaaggc cacgccgagg cctgtcgtca ggctgcgcag caattcctgc
    24301 gactggctgg gatcgaagct ggtggtgtga ccgatctgca gcgcgaggat cgagaagccg
    24361 atcaccttgc ccagcaatcc cagcttcagc tgaatgccgt tgacccacca cgcggtgctg
    24421 tgtggcccgt gcgtcttgtc gagcagcaga tcgagaggcg cgccgctgtc gcgtgcgggg
    24481 ccggcgatgg cgctccagta agcggcggcc catcccgcct cggccgtcgc ccgaccgggc
    24541 ccggcgagtt gcgccgccag catcgcctga cgctgctgct gcagtgttcg gcttcgcgcg
    24601 ccgcaccaca gcgtggcgcc ggcaaacacc accatgatga ccagcgtgat accggtcggg
    24661 tcggcctgca acagcatcgc gaacacgccg cgcacgccga gcagccagct ggcgaatatc
    24721 agcaggccgg ccagcgtgag ccactccagc cacaggggct cggtcatcgg ctctcgcgca
    24781 gccgacgcgg agccgtgggc tggctgccgg cgactggaca tcagcaggcg gttcatcatg
    24841 gaactcatgc ggatgatcct gccgcgcggc acgcgccgcc ttgataggcg cctgaggcga
    24901 agcaatgcag gattgggcgg caactgcgcg ccggcaggga gttcacgatg tgcgttccgg
    24961 aaacgtgcgt ctgtgacgcg atggagcgat gagcggactc aaggacgaga tggcttcggc
    25021 agcgaatgga actgcacggc cgcgattatg caaacccatg gcgcgcaaac tccctgcgga
    25081 atcgccggta ttcgacttga caaaccgatg ccagcccgct ggatttccgg gcctgcgcga
    25141 tcggcaccga accggaactg caacgtgcgg caatcagtcg cggctcatga ctggttccag
    25201 cgcagcttgc ggtagatcgt gttgcggctg attccgagtc gcttggcagc ctcggagatg
    25261 ttgccgcgcg ccgcgtcgag tgcgttgcgg atcatctcga cctctccttc ttccagcgtc
    25321 ttcgcggatc gttccgcagc ggcgggcggg ctgacctggg gaactggcgc gtcaccgtcc
    25381 gaggcttcag ccgcgtttcg cactgcgaat gcgcccccgc gctcggacac ttcggaacgc
    25441 gccccgaatg gctccgggat gcccgtgttg cgtacagatg tcgtgggcgc ctcagccgtg
    25501 ctgctggcga ttgcacgcgg cgcagttccc cgctgcacgt cctcgaggaa atcctcggac
    25561 aggtgggttt cgttgatctg ggcctcgccg gcggccatca cggctgccgt gcgcaacacg
    25621 ttgacgagct gccgcacgtt gccaggccag ccgtagcgac ggaacaaggc catcacggat
    25681 gggcaaacct gaggcgggcg ctcaggagcc tcggcggcca ggatgcgttt gaccagcgca
    25741 gccagatcgg tgcgctcgca caaggcgggc agccgcacga ccagaccgtt gaggcggtag
    25801 tacaggtctt cacggaactg atgccgctcg atcatgccgc gcaggtcgcg gtgcgtcgca
    25861 gcaatgacgg cgatgtccac ggggatcgcc ttctgcgccc ccagcggagt gacgcaacgc
    25921 tcctgcagga cgcgaagcaa acgcgcctga agggccatcg gcatgtcgcc gatctcgtcg
    25981 aggaagagcg tgccgccgtc ggcctgcgcg atcaggccga tcgacccctt gcgtcgtgcg
    26041 ccggtgaagg cgccctcctc gtggccgaac agctcggatt cgatcaacga ctcggggatc
    26101 gaagcgcaat tgaccgcgac aaacggctgt cgagcgcgct gcgaatcatg gtgcgccgcc
    26161 cgcgcgagca cttccttgcc ggtgccggtc tgcccgagta tgagcagcgg aatatcgcgg
    26221 ttcagcacgc ggcgaacctt ctcgaccgtc gccgcgatgc gtgcatcacc ggtctccagc
    26281 cgcgccaggc cgatccctgc cagcttgcgc ggcgcagcgg cggccttagc catgcgctcc
    26341 gaggactgct gctcgggcat ctcgtccgga gcacgaggca acgcgacggg ctcgacagca
    26401 tcgccgtcga cttccgcacc gctgacatcg atccagttga cggcgccgct gaccacggca
    26461 cggggtggcc acacgaagcg cgcactgacg aagaacggcg tgccacacgg gccgttgagc
    26521 tgcatcggca ccgccagtgg cgagcggaaa tgatcgaaga ccgatgccgc gctggccccg
    26581 aacaggctgg tcaggctgtg catgcgcagc gaggcgccgc tcaagccgag caattcgagc
    26641 gcactgcgat tggcgccgat gatcttgcca tccgacccga ccaccaccac gccttcgacg
    26701 agggtgccga ggtaggacac gctgcgatgg aaatgcaggc ggagtcggtt gctgtagtcg
    26761 tcgtggagcc actggttctc gatcatccgc gcggacatgc gcaccagtcc catcgtgtgg
    26821 cggtggtagg tgcgctggtc gccgctgaca tccagaacgc cgagcatgtt gccccgcggg
    26881 tcgaagatcg gcgtggcgga acaggtcagg aagttgtggg catgcatgaa gtgctcgccc
    26941 gcgtgcacga ccgtcggcgc ctcggcgaac agcgcggtgc cgatcgcatt cgtacccttc
    27001 gagtgctctg cccagttggc tcccggcgcc agggcgatct tgtcggcacg ctcgaggaag
    27061 tcgttgtcgc ccatcgaatg caggatggtt ccctgtgcat cggtcagcac gatcatgctg
    27121 tggctgtcga tgatctgttc gaagagcatc tccatcaccg gcgcggcatg cgtggacagg
    27181 cgctggttgc gctcgcgggc cacgttcagg tcgccgcgca tcaacggctc gttgtcgggc
    27241 cgctcgatgc gcgacaggcc gagtgcggtg cagcgcaggt gcgattcctc gatctgcgcc
    27301 agatgggaac gcaggtcagc cgactgcagg ccggtggcgc ttgctgacgc cacgctgacg
    27361 gacgcaggct tgatgcctcg atgcgaagct gccatggtgg ctctccagaa tgaccctcat
    27421 cgtcgccggg caagcgcggg ctgtccatcg gcgcatgccc ggagtggcat gaagcacagc
    27481 ggggcctacg ctgcgctcgc aaacacctgc aaaatcccgg tgcgacggtg caaccaacgg
    27541 ctcaggcccg gcatcgaaac caggcacgaa gacaccgcgc accgggcttg gcacatcgtt
    27601 tgcgcaccgt ccgggctttt gttcccctct ggagacttga tgatgttgga ccctagaaac
    27661 cccgatacgg caactgttgc gaaacggatc agatctgttc cgatgcgcca cagcgtcaca
    27721 ctcgccgcca cccggctcgt ccgccggacc ggcctgaccc tggcggcggt ctgcctgatg
    27781 ggcttgggta ccagtgcagc ctgggcccac ggtgatgtga cgccacagtc ggtggacacc
    27841 aaggcactgc cgccactggg cgccgactgg cttgccgaga acccatatcg cggaaatgcc
    27901 gccgctgtga agatcggcga ctcggcctac aaccagaact gcgcacgctg ccatggcctg
    27961 caggccatct ccggcggcat cgctcccgat ctgcgcaagc tggacaacga ttgcgtctcc
    28021 ctgcaagacg tcaagaagaa ggccgaatgc gtcaaggatg tcgacgtgta ctacctggga
    28081 agcatccgca acggcaaggt tcgcaacggt gccgtgtaca tgcccaagtt cgaaggggtg
    28141 atgaaccagg aagccatgtg gtcgatcaag gcctacctcg agaccgttcg cgagcgcccg
    28201 ctgtgaccac gcacggaggg cccgccgact ggacccggcg tcgctgcctg caggcggcgg
    28261 ctggcgccag cgccctggcg ctgctgccgg cacgtgcgga cgacgacgag ccggatccac
    28321 tggaaaagat ccgcaagcgc gggtcgctgg tggtgggcct gtacaaggat ctgccgccct
    28381 tccacgtcgc tggcgccggc atcgacgtcg acctgtcgca ggccctggct gccgacatgg
    28441 ggctcaagtt cacggcgttg ccgttcgacg ccggcgagaa catgcaggac gacctgcgca
    28501 acatggtctg gaagggccat tacatgggat acggtccggc cgacgtgctg atgcatgtgc
    28561 cggtcgacaa ggcgctgatg aacagctcgc cgcaggtcga gatcttcggt ccgtactacc
    28621 gcgaacggct ggcgatcgcg cgcaacctcg agaaggtgcc tgtgctcgat tccctgtcgc
    28681 gcatcgccgg cttgcaggtg gcggtgcccg gacagtcgct ttccggctgg ttgctgctgg
    28741 gcgcagagaa cggcgcctac cgcgagcaga tgcagacgcg ctgggctgat ggagccgaag
    28801 ccgcgcaggc gttgatgcgt ggcgatgtgc cgctggcagc gggcatggtg tccgagctcg
    28861 aatcggtcgt gcggggcgac acgcgtttcg cgatcgagcc gttgccgctg ccacgttccc
    28921 cacgcgaagg ctgggccgtc ggcatggcgg tcaagaagga ctcgatacgc cttgcgcagg
    28981 ccctgcagtc ctcgttgaac aacctcgtca agaacggcaa ggtggccgaa ctgttcgcga
    29041 agcactcggt gtcctggaaa ccggtctgaa gccggtttcc atcgggcgat caggccgccc
    29101 ggttcaactc ttcgacgact ctcgacgacg cgtgaacggc tgctggtaga gccagaccgt
    29161 gcgcccgtcg ggacaggcat ggacagcgtc cggctgaagc tggcgagcct cgaattccag
    29221 gcttgccagc cagctgcctg aaggcagttc gcggcaagcc ttctcgaccg cccgaggcat
    29281 gctctcgggc cgctggaaca gatagacgag ttgaaacgcc gaccagtcgg tcttccacag
    29341 gtcgccgcgc gtcacgccgg caaagcggca gcgccgctga cacgcccagc gcagcggcca
    29401 gctccactcg atgccctcga tccgcgccgt cgggtactcg gcgtgcagtt cgcgcaagcc
    29461 gtcgccgaga ccacagcccg cttcgagcac gcgcgcggca tcgggcaacg gcaccaggcg
    29521 ctgcaggccc tccagcgcgc cgcgcggcgt cggaaagaac ggcgcatcgc gccaggcatt
    29581 caacgggtag agcagcgcca gaaccgtcag aggcagcaac caggcccagg cgggcacgtc
    29641 gtgcgccgaa ccggacaacg ccagtgaaag cggaaagccc agtgcgatga acaggcgacg
    29701 ccagcgggtc agaggccagg gggacaacac cgcaggcagg gcgccgacca cggccgcgca
    29761 ggccagcgcc acgatcggtg gcgctgcgac agcgctcagc agggcgaaaa cggcccaggc
    29821 gaacgcccag gtcagcaagg caggcagcgg ccagggcagc atcgcaacag gcgatggcag
    29881 ccgcggcgcg ggttcagtat tcgcgcagct tgacacgcag gcgcgcgatc gactggctgt
    29941 gcagctggca cacccgtgac tcggtgacct tgagcacggc agcgatttcc ttcaggttca
    30001 tgtcgtgctc gtagtacatg ctcatcacgt actgctcgcg ctcgggaagc accttgatgc
    30061 cggcgaccag cgcctcgcgc atgcgctgat cctgcaactg ggccagcggg ttgaacgatt
    30121 cgtctgccac gtggcggtcg aggtagtcgt ggtcgccgtc atcgcccgac atgtcctcga
    30181 ggtagaccag ctgcgtgcca cggaccttgc ccagcagttc ctggtactcg gtcagcgtga
    30241 tgcccatttc cttggcgatc tcgctttcgg cgggggcgcg gcccatgcgc tgctcgagct
    30301 tgtggacagc cgattcgatg gtgcgctgct gcttgcgcgt gccgcgggac aggtagtcgt
    30361 tgccgcgcag ttcgtcgagc atggcgcctc ggatgcgctg cgtcgcgaac gtctcgaact
    30421 ggacgccctg cgccgcatcg aagcgggtca gcgcatcggc caggccgatc atgccgacct
    30481 ggatcaggtc gtcgagttcg acgttggccg gcagcttggc aatcatctgg tgcgccaggc
    30541 ggcgcaccag tgggctgtac tgcttgagca tcgacgtgtt gtcgagctgt cctttggcgg
    30601 tgtacgtgga catggtcggc tccggagatt cagttgagcg cgtgtgcatg gtgctcattc
    30661 gctgcagttg gcaagacggc cagctcgccg gtggcgggcg ccagggtgtc tcgaacccag
    30721 cgtcgcatgg caggcgtcgg ggcactgcgt gcatcgctgg ctggatcgat ctgcatccag
    30781 tcgcgcagca cggcgcccag gaaatcgtca gcgcaggtcg cgatctgcat cgcgatgcgc
    30841 tcggcgcggg gagaactcgg tgcggcgctc agcagcaggt cgtgcaccag caggccagca
    30901 cgctgcgtca gcagcttcat cgcggcatag gcatgcgtca cgctgaccgg gcggtcgtct
    30961 gccagcagca gagggcgagg ccgcgtgctg gactcgcgtc gcatgaacag ccggcacaga
    31021 tcggtcgcgc tggcatgcac cagcaccaca tcggcctccg gcgcggccag cgagaccgcc
    31081 tcgaggaagc tggccgtgga accattcgag tcgacgtagc gcagcggcag gccgcgcgcc
    31141 gcgaggtacg agacttcctg cgaaagcacc tcgatgcatt cggccaggtc gacctctgcc
    31201 agctcgttcg gaccgggagc gttctcgccg gcatcgacaa ccagggtgtg gcagccctgt
    31261 tcggccaggc cgctgcacag gcgttcgagc atcacgccgc cgaaggccat gtgcgggttc
    31321 gagaccacgg gcacgaatcg aacctgggca tgcgcgaaca atcgccgcag cccatcggcc
    31381 tgatccagcg gcatcgagga ggaagaaggg gtgaaggaag gcgtcatcgc ggaaaggctc
    31441 tgggtaggct gggtcgcgga gggcttgcgg gtgcgcagcg ggcgcggcat caggcgtgca
    31501 gggccatggc gtcgtgcggg acggcctgct gcagcgggtg cgactggtgc tgcggtgcag
    31561 cgaagatcag gttcacctcg ctcgaatcca ttcggtaggc actgccgacg gtcgggcgca
    31621 gcgcccggtg gatcagtgcg tgcgacgaca gtcggtgcca gtcctccggc acccgctgtc
    31681 cattggccac accgaccacg gtgagcttgt ggcggatcag cgcgtcgagc gccgggccga
    31741 gcttgacggc ctcgtccatc ttcgagagca cgatgccgcg gcaggtgctg gcgtgatacg
    31801 agatcagcac gtcctcgatg gtctcgccct gcgcggacgc attgaccacc agcagtttgt
    31861 ggatcgaccg gtgactgagc atctccagca attcgcgggt gcgcgtgtcg cgctgcgcca
    31921 tgccggcggt gtcgatcagg accatcttct tgccggcgag caactcgagc aggtcttcca
    31981 gcgaagcgcg gtcgtgcgcg gtgtgcaccg gcacgccgag gatgcgcccg taggcacgca
    32041 gctgttcgtg tgcgccgacc cggtaggcgt cgagggtgat caggcccagg tgcgccgcgc
    32101 cgtagcgggt cgcaaaggcc gctgccagct tggcggtggt ggtggtcttg ccgacgccgg
    32161 tggccccgac cagcgcatag acgccgccct ggtcctcgag tgccggctcg cggtcggagc
    32221 tcagcagatt gcgctcgagc acgccggccg cccagcccat ctcgtcggtg acttcggtgc
    32281 ccatgccttc ggcgagcttg cggatcagcg cgggcgagaa gccgcagtcg atcagcttct
    32341 gcgtcaaccg cgcctgcgca ggctggcgct gcagcttttc catgaaggcg agtgcgccga
    32401 agcgttcctc gatcatgcct ttcatgctgc gcagttcgct catcatgtcc tgctggtcgc
    32461 gccgcgccgc gctggcatcg gcagccaggg gagccgtcgc agccggcgcc acacgggcac
    32521 ggccgcgcgc ttcggcaagc gtgcgcactt cctcatgcag taccggcggc tcgtgcagca
    32581 ccggcatggc gttgacatac atcgggtcgg tgtcgctgtg cagcgccgac agcaccgggg
    32641 catggcccgg cgtgggctgg gccactcgtg acggaccgtt ggacgccgga ggcgtcgaac
    32701 gtgccacggg cgcatcggcc atgcgggtgg ccagcgggcc gggcaccggc tcgaccaccg
    32761 gctcggcggc gcgcgccgcg gccgattcgg cctgcaacgc gacctgacga cgcttgagca
    32821 tgcgctcgcg cacatagtcc tggaacgaca gcgtgctcat cgacaggcgc tcgacgtcgt
    32881 cctcgaccgt cgtggcgtcg gcggcgggct gcggctcgac acgcgcggcg agctgggttc
    32941 gggcgacacc cggacgcccc gggccgggac gaccggccgc cggacgttgc gcagcgggcg
    33001 tcctggccgt cggcgcggcc gcaggcgttg aatcggtggc catgcgctcg atctgagcca
    33061 tgctctccgg cgccatcgcc agcacctcga caccttcggc agacggccgg gtggacagca
    33121 cgaccgcgtc ttcgccgaga gcctggcgca ccagggccag tgcgtcgcgc gatgtgcgcg
    33181 cggtgaaacg tttgacgttc atgcggtacc tcctatgacc gacccgatgc ggatcgaatg
    33241 cgtttccgga atttcactgt gtgccagcac ccgcatgcgg ggagccgcac gcttgagcag
    33301 gcgggccatc ggcgcgcgga tcaggtccgg caccagcagg caggcgggca gccccaggtc
    33361 ttcctggcgc ttggccgaat cggccgcgtg gcgggcgagc gtgtcggcca cgccggggtc
    33421 gagcgccgcg ccgtgcggcg aattgagcgc ctgcgtgacg agccgttcga ggtcgggttc
    33481 gagtgcgatc acgtcgagct ccttgctcgg gccgtagatc tgctggacga tggacggcgc
    33541 caggtggatg cggatgcgcc gggccagttc ggccgggtcg gtgacggtgg aggcgttctc
    33601 ggccaggctc tcgacgatgg agcgcatgtc gcggatgtgc acgccttctt ccagcagcag
    33661 ctggagaacc ttttgcagag ccggaatcgc aaccatcttg ggcaccacgt cctcgatcat
    33721 cttgggggcc agcttggtca cgtgttcgac caggttctgg acctccaccc gacccaacaa
    33781 cttggccgcg tgaagttgca tcaagtgtga aagatgggtc gccacgacgg tcgagcaatc
    33841 aaccaccgta aacccattca tttgggccga ctcacgctga cgctcctcga tccagaccgc
    33901 cggcaggccg aaggccgggt cggtggtgcg cgtgccgatc aggttggtgc tcgcgccgcc
    33961 cgggttgatg gccagccaca tgcctgggaa ggcctcgccc tcgcccacca cggcgccacg
    34021 cagcgtcagg cggtacatgc tcggcttgag ttcgaggttg tcgcggatgt gcaccggcgg
    34081 cggcaggaaa cccacgtcct gcgcgaactt gcgccgaacg cccttgatgc gcgccagcag
    34141 atcgccttcg cgcgccttgt cgaccagcga gatcagccgg tagccgacct ccaggcccag
    34201 cgtgtcgatc ggcagcaggt cgtcccaggt cgcctcggca ttggcttccg cggccatggc
    34261 cggtgccggc ggcggcgctt gtcgggccac ccggtcgcgc tcgcgcatcc agtaggcggc
    34321 gtagccgagg ctggccgcga tcagcaggaa caccacgtgc ggcatgcccg ggatcaggcc
    34381 cagcgtgccg atcacgccgg cggtgatggc cagcgcgcgc ggcgagttga acatctgcgc
    34441 ggcgatctgc gttcccacgt cgttgccctt gccgacgcga gacacgacca tcgccgccgc
    34501 gaccgagatc agcaggcccg ggatctgcgc gaccagcgcg tcgccgaccg ccagcacgat
    34561 gtagctgtcc gccgcctctg cagcagacag gttgtgctga gcgacgccga tgatgaagcc
    34621 gccgatgatg ttgatcacca ggatcagcag gcccgccatc gcgtcgccgc gcacgaactt
    34681 gctggcaccg tccatcgagc cgaagaactc cgcttcgtcg ccgacttcgg cgcggcggcg
    34741 cttggcctcc ttctcgtcga tcaggcccgc gttcaggtcg gcatcgatcg ccatctgctt
    34801 gcccggcatg gcatccagcg tgaagcgtgc gccaacttcg gcgattcgct cggcaccctt
    34861 ggtcaccacg acgaagttga tgacgaccag gatcgcgaag acgatcaggc cgaccgcgaa
    34921 gttgccgccg atcaggaaat gtccgaacga ttcgatcacc gcaccggctg cgcccggccc
    34981 ggtgtggccc tccatcagca ccacccgcgt ggacgccacg ttcagcgaca ggcgcagcag
    35041 ggtggtcagc aggatcaccg tcgggaatgc ggcgaagtcc agcggcttga ccatgtaggc
    35101 ggccaccatc atcaccatca gcgccatcgc gatgttgaag gtgaacagca ggtcgagcgc
    35161 gaacggcggc aatggcagca ccatcatcgc cagcaccatc acgatgaaca cgggcgccat
    35221 cagcgccttg atggcggacg cgttcgggcc cagccagccc ttgagttgct gcagcgggtt
    35281 cattcagcgt ctttctcggt ggacggtgcg ggcggcacgt agtccggatc gttgtggggg
    35341 tcgagctcgg gcggcaccga cagttcgggc aggtcgggcg gcatggcccc acgaccgctc
    35401 atcgcggcgc gcaactggta cacataggcc agcacctgcg cgacagcggc gaacagggcg
    35461 gtcgggatct cccgatcgac ctcggcatgg gcgtagagcg cgcgggccag caccggggcc
    35521 tggagcacag gcacctgggc cgcacgtgcg gtatcgcgga tcgtcatcgc aaggatgtcg
    35581 gcgcccttgg ccacgacgcg gggtgcaccc attcgcgctt cgtcgtactt gagcgcgacg
    35641 gcgtagtggg tcgggttcat caccaccagg tcggcagtgg gcacggcagc cagcatccgg
    35701 cggcgcgaca tctcgcgcat cagctgcttg cggcgtccct tgacctcctg gctgccttcg
    35761 gattccttgt attcctgctt ctgctcctgc gcgctcatct tcagcttcga cgcattcagg
    35821 tagcgctgca gcggtgcatc gacgaccgcg aacagcgcca gcacgccgag cagcagccag
    35881 agcccgccct ggatggccga gcccgcctgg gtcagcccgg cgctcagcgg catcgacacc
    35941 aggccgcgaa actcgtcgac gtggctgtag agatagacac agccgacggt gccgaggagc
    36001 aaggccagca ccgacgcctt ggtggcatcg accatctgct gcttcgagaa catcctgcca
    36061 agtcccgcga tcggcttgaa cggggtgccc ttcggcatca gcggcttcag cgtccagttc
    36121 cagccgccga cggccagtgc gctggcaatg ccgagcgctg tcatccacaa ccccatgggc
    36181 acgacgaccc acagcatcat cgcgaggagg tcggcgagca gatcgaacat caccgaaggc
    36241 gcgccccggc tgccggcacc gaagctcagt ccccgggaca ggccttcgcg ggtccagccg
    36301 accagcgtcg gcatcatcac gaccagcccc agaccgccgc cggcgagcgc cgcgaagtgt
    36361 ccgaaatcgc gcgagcgcgg gacattgccc tcctcgcgcg ctttcttcag cttgcgtgcc
    36421 gaggcgggca gattgcggtc ttgagcgtct tggtcggcca tgggcgggca cggggggcag
    36481 ggtcacgcag gggtcgcgtc aaccgattgt tcccagcccg cccgcaaact tgagcgagag
    36541 aaggggggga aacgcgcccc ttctcgaacc cctgcccggc gcaccacccc ggtacggacc
    36601 cgcgcgggtc gatggcacga gcctcgcgct ttgcaaccct cgctaacccg cgctgcccat
    36661 cgattgcgac tacgtagttt ccgagggcag cgcagccgga cgcgccagcg cttcaggtcg
    36721 ataattttgg acgtgtcgcc cggaacggcg gccgtcgaag aatcaggtca acaacgcatt
    36781 ccaggagacg caccatgagc acccccgaca agaaagccgc cccccgccgc cggttcctgc
    36841 agcgggccgc caccggcgcc gttgccaccg gcgcgctggc cgcaccgatg gtttcccgcg
    36901 cgcagaccac gagcctgcgc ttccagagca cctggccagc caaggacatc ttccacgagt
    36961 acgccagcga tttcgcgaag aaggtgaacg acatggcggg cagccgcctg aagatcgagg
    37021 tgctgccggc cggctcggtc gtgccggcct tccagctgct cgatgcggtg agcaagggca
    37081 cgctcgacgg cggccacggc gtgatcgcct actggtacgg caagaactcg gcgctcgcgc
    37141 tgtggggctc gggcccgggc ttcggcatgg acgccaacac cgtgctcgca tggcacaact
    37201 acggcggcgg caaggaactg gtcgccgaga tctacaagag cctgaacctc gatgtggcct
    37261 cctacatgta cgggccgatg ccggttcaac cgctgggctg gttcaagaag ccggtcgcca
    37321 aggtcgagga catgaagggc ctgaagttcc gcacggtcgg cctggccgtc gacgtgttca
    37381 ccgagttggg caccgcggtc aacccgctgc ccggcggcga gatcgtgccg gcgctggacc
    37441 gcggcctgat cgatgcggcc gagttcaaca acgcca
//
LOCUS       DAOORV010000016        37203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1288_length_37203_cov_7.192931, whole genome shotgun sequence.
ACCESSION   DAOORV010000016 DAOORV010000000
VERSION     DAOORV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 37203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_37203_cov_7.192931"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PKV56_03890"
     CDS             <1..1485
                     /locus_tag="PKV56_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX66983.1"
                     /translation="RTALPASREAHRYLIQLLVAMNRSSETPEPLRSLIKLTSAAERP
                     GLIASLPRFFERTSDSKAVPEIIEAALKSSVDEPETRTASLVSIGRSWLTAGNNAKAL
                     DYAQRAHVQDKSAEGPVLLAVAVLPVVAEAEPVIVGYLQANPRASAIRMIYARALSVS
                     QRHAEALAQVETVTRDEPGQSAPWLTLGALHLELRQPKEATAALKTFIDRVQAAPAPT
                     PPSPDAAAVPAEEEQVVADPDRGLTQAWLMLAQAAELQGDFKGAEDWLSKVQMPQRAL
                     EVQVRRASLLARQGRLAEGVELIRSAPAGSEDEARAKVLAEVQLLREARQWAQASAVL
                     SEANQRFVDDPDLLYEQSMMEEKLNNVAEMERLLRRVIALRPDHHHAHNALGFSLADR
                     GLRLPEAKALIEHALQLAPGEPFITDSLGWVEYRMGNQAEAIKQLTRAYQSRPDPEIA
                     AHLGEVLWVSGQRDEARRVLREGTRRDQANDVLRETVTRLKVDL"
     gene            1587..2018
                     /locus_tag="PKV56_03895"
     CDS             1587..2018
                     /locus_tag="PKV56_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HOX66984.1"
                     /translation="MSVQVAATETTEAHSMSAAFELSGTPDRGRLDLNTPIGSTVARA
                     RWKSDSVVLDTPQGQTRHATLADMTRAVVGEELPVPAMFDWLRGRPWPAAESTPSMAP
                     APTGFRQLGWTVDLSRFDEARILARRDAPPAVTVQVKLDKP"
     gene            2080..2964
                     /gene="ispE"
                     /locus_tag="PKV56_03900"
     CDS             2080..2964
                     /gene="ispE"
                     /locus_tag="PKV56_03900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOX66985.1"
                     /translation="MRTLLDVPAPAKINLFLHVVGRRADGHHLLQSVFVLIDWADTLH
                     FERRSDARLARHDLTESLPADDLCLRAARALQSASGTAWGADISITKQVPWGAGLGGG
                     SSDAATTLLALNRLWGLNWSRSKLHALGLALGADVPFFIGGQNAWVEGIGEQLRPIAV
                     PMQTFMLIKPPVSIETRAVFTHPGLARDTPRAILEGFDADAFQDLVAPKAGAGWGRND
                     LQVVAESLHPEVHEAVRQLQERFGNGRMSGSGSAVFARTGTSGVAAGELEGGLPAGWI
                     GRRCRSLVEHPLRTWAAD"
     gene            3010..3086
                     /locus_tag="PKV56_03905"
     tRNA            3010..3086
                     /locus_tag="PKV56_03905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3044..3046,aa:Gln,seq:ttg)
     gene            3148..4140
                     /locus_tag="PKV56_03910"
     CDS             3148..4140
                     /locus_tag="PKV56_03910"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOX66986.1"
                     /translation="MLPQSLVGGNVLFNTVLFTGNANPVLAQEIATHLGVELGKASVG
                     RFSDSEVAVEIEQNVRARDVFVVQSTCTPTNENLMELCIMVDALKRASARRITAVIPY
                     FGYARQDRRPRSTRVPISAKVVANMLEAVGVERLLTMDLHADQIQGFFNIPVDNIYAS
                     PVLLSDLQSKQYEDLVVVSPDIGGVVRARALAKQLNCDLAIIDKRRPKANVSEVMNVI
                     GETEGRNCVIMDDMIDTAGTLVKAADVLKARGAKSVYAYCTHAVFSGPAIERIRSSML
                     DEVVITNTIPMTDAAKVCPKVRQLSVAFLFAETIRRISDGESVTSLFAEQNSNF"
     gene            4243..4887
                     /locus_tag="PKV56_03915"
     CDS             4243..4887
                     /locus_tag="PKV56_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOX66987.1"
                     /translation="MKFVAYERTLQGTGASRRLRNADKVPGIVYGAGTPAKIELDHNA
                     LFFALKKEAFHSSILEMELAGKTEQVLLRDYQFHPFRPIVLHVDFQRVDATTKITKKV
                     PLHFINEDESPAVKVDKCVVNHVVTELRIQCLASQLPEFLTVDLGQLVKGQSLHVNDI
                     SLPAGIKVMTQGKPNPVLVSASMLTAEEEAAPVVVTAAAAAPTKGKKTEKQNKK"
     gene            4970..5587
                     /gene="pth"
                     /locus_tag="PKV56_03920"
     CDS             4970..5587
                     /gene="pth"
                     /locus_tag="PKV56_03920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOX66988.1"
                     /translation="MIRLMVGLGNPGQEYEGTRHNAGFWFIDEVARHLKVTLAPDRSY
                     FGLVARVNLPGGPLWLLEPMTFMNLSGKSVAPLARFFKIEPEDILVAHDELDLMPGQM
                     KMKLGGSHAGHNGLKDIQAQLGSPGFWRLRLGIGHPGVKAEVVNHVLRKPPLAERQAI
                     DQSIAQALGALDLMIAGDMERAMVKIHARPPRPKKPPESNERPAS"
     gene            5584..6015
                     /locus_tag="PKV56_03925"
     CDS             5584..6015
                     /locus_tag="PKV56_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX66989.1"
                     /translation="MKLLAMSCALLLTAVASWAAAGEIYRCGPAGAEFSQAPCANGAR
                     FEVSDSRTADQYSQAVVLAAKFEAWGSAMERDRLATEASYRPTLAVSLNSSASGTTEY
                     RPRSASQPKRKRVKAVSAGVPLLSHGRPRFDRDPDADFSRR"
     gene            complement(6002..6295)
                     /locus_tag="PKV56_03930"
     CDS             complement(6002..6295)
                     /locus_tag="PKV56_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOX66990.1"
                     /translation="MALLITDECINCDVCEPECPNSAISMGIEIYQIDPARCTECVGH
                     FDEPQCVQVCPVNCIPVHPDHVESNESLWIKYRRLQAAARTEASNGTASAIAD"
     gene            complement(6314..6817)
                     /gene="coaD"
                     /locus_tag="PKV56_03935"
     CDS             complement(6314..6817)
                     /gene="coaD"
                     /locus_tag="PKV56_03935"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOX66991.1"
                     /translation="MTSVTKLTAIYPGTFDPMTLGHEDLMRRASRLFERLIVAVAAGH
                     HKRTMFTIEERLDIAEELARKHPNVEVIAFRGLLRDFVVGIGGKVVVRGLRAVSDFEY
                     EFQMAGMNRQLMPDVETVFLTPSDQYQFVSGTFVREIASLGGDVSKFVSPSVLDRLRS
                     RVKYTEA"
     gene            complement(6831..7592)
                     /gene="rsmD"
                     /locus_tag="PKV56_03940"
     CDS             complement(6831..7592)
                     /gene="rsmD"
                     /locus_tag="PKV56_03940"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOX66992.1"
                     /translation="MASIISKSVPRPKAAAKVAAKADPKASLRFGSRAYTESPSVKKA
                     HKKAADKPPVAPDTASLPREVRIIGGVWKRSKLPVILLPGLRPTPDRVRETLFNWLGA
                     DLSGWRCLDAFGGSGALGFEAASRGAASVVMLEREQRQVRSLGESKLRLKADMLRIEA
                     TDALSWMRRCVPDSFELVFIDPPFGSDLLGPALAAAARLVVPQGYIYLEAAHAYSGEE
                     VAPLGLTVHRHFRAGLVHAHLLQRAAADYTATITA"
     gene            complement(7592..8371)
                     /gene="hisF"
                     /locus_tag="PKV56_03945"
     CDS             complement(7592..8371)
                     /gene="hisF"
                     /locus_tag="PKV56_03945"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOX66993.1"
                     /translation="MLCKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNDQGA
                     DELTFLDITATSDGRDLILHIIEAVASQVFIPLTVGGGVRSVEDVRRLLNAGADKVSF
                     NSAAVANPQLIRDASGRYGAQCIVVAIDAKRRVGDDLALRGPGWDVYTHGGRKNTGLD
                     AVDWAAQMTEHGAGEILLTSMDRDGTKSGFDLPLTRAVSDRVEVPVIASGGVGALEHL
                     SEGIRVGGADAVLAASIFHYGEFTVGQAKALMVRDGIPVRL"
     gene            complement(8424..9167)
                     /gene="hisA"
                     /locus_tag="PKV56_03950"
     CDS             complement(8424..9167)
                     /gene="hisA"
                     /locus_tag="PKV56_03950"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOX66994.1"
                     /translation="MLLIPAIDLKDGKCVRLKQGDMNDSTTFGEDPAAMARRWVDAGA
                     RRLHLVDLNGAFAGKPVNESAIKAILAEVGDEIPVQLGGGIRDLDTIERYLDDGLSFI
                     IIGTAAVKNPGFLRDACSAFGGHIIVGLDAKDGKVATDGWSKLTGHEVVDLAKKFEDY
                     GVEGVIYTDIGRDGMLSGINIEATVKLAQALTIPVIASGGLSNLADIDRLCAVEFEGI
                     DGVICGRAIYSGDLDFAAAQTRADELSGT"
     gene            complement(9267..9926)
                     /gene="hisH"
                     /locus_tag="PKV56_03955"
     CDS             complement(9267..9926)
                     /gene="hisH"
                     /locus_tag="PKV56_03955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOX66995.1"
                     /translation="MGKTVAVVDYGMGNLRSVSQAVMHVAQGSGFEVLVTADPDVVYA
                     AERVVLPGQGAMPDCMRELRDSGLKEAVLDAAARKPLLGVCVGMQMLLSHSEEGPPGE
                     GTAGLGLMQGDVIRFRLDGQLQPDGSRYKVPQMGWNQVIQVRPHSLWAGVPDGASFYF
                     VHSFYARPADRAHSIGETDYGQRFTCALGRDNIFATQFHPEKSALHGLAVYRNFLHWN
                     P"
     gene            complement(9929..10549)
                     /gene="hisB"
                     /locus_tag="PKV56_03960"
     CDS             complement(9929..10549)
                     /gene="hisB"
                     /locus_tag="PKV56_03960"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOX66996.1"
                     /translation="MSPDTTAPALSHRRAEVARNTAETQIRVRLDLDGTGVGQFATGI
                     GFLDHMLDQIARHGLIDLNVEAVGDLHIDGHHTVEDIGITLGQAVATAVGDKRGLRRY
                     GHAYVPLDEALSRVVIDFSGRPGLHMRVDFKSGMIGALDTQLVYEFFQGFVNHAGVSL
                     HIDNLHGDNAHHQCETIFKAFARAMRMALEIDPRMGNVIPSTKGSL"
     gene            complement(10546..11643)
                     /gene="hisC"
                     /locus_tag="PKV56_03965"
     CDS             complement(10546..11643)
                     /gene="hisC"
                     /locus_tag="PKV56_03965"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOX66997.1"
                     /translation="MSPPTTSDVIQRHIRGDVRAMLAYAVQPSDGLVKLDAMENPFRL
                     PEPLRAALGARLAEVAINRYPGSRNNDLRCALAQHAGLPEGFDLILGNGSDELISMLS
                     LACATPGASALAPVPGFVMYDVSARLQGLRSISVPLRPDFELDESAMLAAMQQHQPAI
                     VYIAYPNNPTANLFDDAIIERVIEAAPGLVVIDEAYQPFASRSYIDRVARHGHVLIMR
                     TLSKFGLAGVRLGYLMGPKALIAEIDKVRPPYNVSVLNCETAMFALEHADEFDRQAAV
                     LRSERSRLTSALHALPGVHPYPSEANMILTRVNDAAAVFGALKNRGVLVKNVSNLHPL
                     LADCLRLTVGTPEENDILLSALKSSLEASPR"
     gene            complement(11643..12971)
                     /gene="hisD"
                     /locus_tag="PKV56_03970"
     CDS             complement(11643..12971)
                     /gene="hisD"
                     /locus_tag="PKV56_03970"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOX66998.1"
                     /translation="MTVRIRHLDTSAAGFAAEFERVLHWSAETDDAIEQRVASILADV
                     QLRGDAAVLEYTRRFDGVEAASVAALEIGHDELHAALAAITPAQRSALEFAADRVRDY
                     HQRQLEACGRSWSYVDADGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAVPAKVAG
                     VGEIVMVVPTPRGEKNLLVLAAAAVAGADRVFTIGGSQAVAALAYGTATVPAVDKITG
                     PGNAYVASAKRRVFGKVGIDMIAGPSEILVLADGSTPAEWVAMDLFSQAEHDELAQSI
                     LLCPDAAYIERVQAEIDRLIEGMPRRDVIRASLEGRGALIRTRSMEEACEISNRIAPE
                     HLEVSSRDPHRWEPLLRHAGAIFLGAYTSESLGDYCAGPNHVLPTSGTARFSSPLGVY
                     DFQKRSSLIEVSEAGAQKLGGVAAELAYGEGLQAHAQAAEMRMKRRDSDS"
     gene            complement(12983..13621)
                     /gene="hisG"
                     /locus_tag="PKV56_03975"
     CDS             complement(12983..13621)
                     /gene="hisG"
                     /locus_tag="PKV56_03975"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOX66999.1"
                     /translation="MITLALSKGRIFEETMPLLQAAGIEVSEDPEKSRKLIIGTNRAD
                     VRVVLVRASDVPTYVQYGGADLGVTGKDTLFEHGGSGLYQPLDLKIAKCRMSVATRAD
                     FDYASAVKQGSRIRVATKYTLMAQQHFSDKGVHVDLIKLYGSMELAPLTGLADAIVDL
                     VSSGNTLKANHLVEVEEIMQISARLVVNQAALKLKREPIRAIIDAFASAIQS"
     gene            complement(13632..14903)
                     /gene="murA"
                     /locus_tag="PKV56_03980"
     CDS             complement(13632..14903)
                     /gene="murA"
                     /locus_tag="PKV56_03980"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOX67000.1"
                     /translation="MDKLLIRGGRPLEGEVTISGAKNAALPELCAALLTAEPVTLTNV
                     PRLQDVSTTLKLLRNMGATAERSEIQPDAVTLDTSTVTSPEAPYDLVKTMRASILVLG
                     PLLARFGEATVSLPGGCAIGSRPVDQHIKGLQAMGAQITVEHGYIIAKTPRLQGARIT
                     TDMVTVTGTENLLMAATLAEGETVLENAAQEPEVADLGDMLIAMGARIEGHGTRRIRV
                     HGVDRLHGLSGAASHRIVPDRIEAGTFLCAVAATGGDVVLRRARADHLDAVIDKLREA
                     GVTIESGEDWIRVRSQQRPRAVSFRTSEYPAFPTDMQAQFMALNCIADGTAKVTETIF
                     ENRFMHVNELVRLGARIDIDGHLAVVHGVQKLSGATVMATDLRASASLVIAGLVADGQ
                     TCVDRIYHLDRGYDRMEAKLRGIGADIERVQ"
     gene            complement(14983..15252)
                     /locus_tag="PKV56_03985"
     CDS             complement(14983..15252)
                     /locus_tag="PKV56_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOX67001.1"
                     /translation="MSDPTPNDVQRYIAQGLACEHLEVLGDGQHFFATIVCAAFEGAS
                     RVARHQLVYRALGDKMRAEIHALSMKTLTPAEWAASSDKAPLQHH"
     gene            complement(15276..16067)
                     /locus_tag="PKV56_03990"
     CDS             complement(15276..16067)
                     /locus_tag="PKV56_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67002.1"
                     /translation="MTTPASATSAHRLSGARTLFYKEVLRFLKVSFQTVAAPVLTAVL
                     YLMIFGHVLEENVQPLSGVGYTSFLIPGLVMMSVLQNAFANSSSSLIQSKISGNLVFL
                     LVAPLSPWAWFVSYVGASVVRGVAVGTGVLAVTAWFAPLQLAQPAWIVVFTLLGAILM
                     GALGLIAGLWAEKFDQIAAFQNFLIMPMTFLAGVFYSVSSLPAIWQEASHLNPFFYIV
                     DGFRFGFFGVSDVSPWVSLCVAGAGLLAVCGLCLHLLRTGYKIRH"
     gene            complement(16064..16996)
                     /locus_tag="PKV56_03995"
     CDS             complement(16064..16996)
                     /locus_tag="PKV56_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67003.1"
                     /translation="MAAVSFRDVSKRFRGSRGTVQALDGVTFDIAPGEFFGLLGPNGA
                     GKTTLINILAGLAQASAGQVLVHGHDVVADYAAARRSLGIVPQELVFDPFFTVRETLQ
                     IQSGYFGVRTDRRANDAWIDELLDHLGLTDKAGANMRQLSGGMKRRVLVAQALVHRPP
                     VIVLDEPTAGVDVELRQTLWQFIARLNKEGHTVLLTTHYLEEAEALCSRLAMLKQGRV
                     VALDRTSALLAGRASTMLRFKLDTPLPPALLAQARVTGRIVQIKTRNAAEVEKVLATL
                     RSAGAQPEDLEIGRADLEDVFLEIMQGEPLQGAA"
     gene            17185..17922
                     /locus_tag="PKV56_04000"
     CDS             17185..17922
                     /locus_tag="PKV56_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX67004.1"
                     /translation="MATPVKKPRRTAERILDVTLELFNRFGEPNVSTTLISAELNIST
                     GNLYYHYPAKDLLVDRLFDRYEQALGSLLKAADDVRDVEDAWLFFHMLFELIWQYRFL
                     YRDLNDLLSKNRRIETHFQFVLQEKNQAVQSVLAGLGRSNAIRLTAAEAESVATAMVV
                     VATYWLSYEYVRDPRHALEPENAGAALLRGASNVLSLLAPYFEAAQKQHLLGLAQAYS
                     KPAAAPVLEAAARRGTTQAATSARAAL"
     gene            complement(17925..18248)
                     /locus_tag="PKV56_04005"
     CDS             complement(17925..18248)
                     /locus_tag="PKV56_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOX67005.1"
                     /translation="MTTFQIHRPHSLGLEPARDLARHWAEAAQQKHQMNCTVREGEVD
                     NRVDFARSGVRGELVASADCFDLTVTLGFLLSAFSQNIRDEIERNLEAAIAQAGAAAA
                     PQSKG"
     gene            complement(18285..19361)
                     /locus_tag="PKV56_04010"
     CDS             complement(18285..19361)
                     /locus_tag="PKV56_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOX67006.1"
                     /translation="MKSGALIRAVLLSAAVSGLATGCSSVGYYAQSIGGHLNLLAAAR
                     PVPEWLADPATPDKLKARLELTRQIRDFAVTELDLPDNASYRRYADLQRAAAVWNVVA
                     APELGLTLETWCFPVVGCVAYRGYYAREEADAYAEQLRARGLEAWVYGVPAYSTLGKL
                     PGAYFADPLLNTFVHYPDGELARLIFHELSHQVAHASDDTVFNESFATAVERLGSARW
                     LSRRAGDAARREFAAFESRRADFRALTGRYRDRLQALYAGAAPDSDKRRGKTELLAQL
                     RAEHAALKAGAWQGYSGYDGWFAQVNNASFGVLAAYNELVPQFEQLLESQGGDLPRFY
                     AEVRRLAALPMAERRQVLLRASPH"
     gene            complement(19358..21448)
                     /locus_tag="PKV56_04015"
     CDS             complement(19358..21448)
                     /locus_tag="PKV56_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HOX67007.1"
                     /translation="MNTRIIRAACPHDCPDTCAMQVTVTDGIAVKVQGDPDHPATHGA
                     LCTKVSRYPERTYHPDRVLHPLKRIAPKSGTGGGARFERVSWDDALRDIAARLSGIAS
                     RDPQAILPYSYAGTMGLVQGESMAARFFHRLGASLLDRTICAGAGTEAMAASYGDTVG
                     MHVEHFVESRLILIWGSNSIGSNLHFWTMAQEAKRQGAKLICIDPRRTETADRCHQHV
                     ALMPGTDGALALGLMHELIVHDRLDHDYIERHVEEGEGGWPALRERAMSWPPARVAGV
                     CGITADEVRSLARDCASMAPAAIRLNYGMQRVRGGGSAVRLIAMLPCLIGAWRHRAGG
                     LLLSSSGWFRGARNDAWLQRPDLLGGPDGRRAMPRTLNMSTIGDDLLRPSGEVLPDGT
                     DFGPRIEAVVVYNSNPVAVAPQSPKVVAGFAREDLFTVVLEHFMTDTADLADYVLPAT
                     TQLEHLDVHASYGHTYALINERAIEPQGEARPNTQIFRELAARMGWDDACFADDDEAL
                     ARGAFTAVVPFEELRARGWIKLPIADAPFAQGGFPTASGRCMISSIKHGMADHVANHE
                     SLASAPALARRYPLAMISPPARNFLNSTFVNLRSLRDIEGEPLLEIHAADAAARGLAE
                     GQPVRVFNDRGSYLCRTAISARARPGVVNGLGIWWRKDGPGGTNVNEVTHQLPTDIGR
                     GPAFYDCLVQVEAA"
     gene            21611..22807
                     /locus_tag="PKV56_04020"
     CDS             21611..22807
                     /locus_tag="PKV56_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOX67008.1"
                     /translation="MRLIESILSDAAAMTALRRDLHAHPELCFAEQRTSDVIARQLSD
                     WGIPIHRGLGTTGVVAILKNGSSDRAVGLRADMDALPMTEQNRFEHASRHAGKMHACG
                     HDGHTTMLLAAAHHLARHRSYDGTVYLIFQPAEEGGGGAREMIKDGLLDRFPMEAIFG
                     VHNWPGMPVGQFAIRNGPCFASSNEFRITIRGKGSHAAMPNLGLDPVPVACQMVQAFQ
                     TIITRNKRPIDAGVISVTMIHAGEATNVVPDTCEIQGTVRTFTLEVLDLIERRMLEVA
                     QHTSAAFGVTCEFEFTRNYPPTINHPAETAFARSVMTDLVGADNVLEFDPTMGAEDFS
                     YFLQALPGAYFVMGNGDGDHRNPGHGLGPCMLHNPSYDFNDDLIPLGATLWVRLAEQW
                     LARPQR"
     gene            22877..23968
                     /locus_tag="PKV56_04025"
     CDS             22877..23968
                     /locus_tag="PKV56_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOX67009.1"
                     /translation="MTELNFFSATYAEARERFVAAATRCDLDIESHPHPLIGPQGEAL
                     ALDVVRDGLRRAPRLLIISSGCHGIEGHVGSAVQTALLSDPAWRAATREAGVAVLYLL
                     ALNPHGFAWGRRATQENVDLNRNFLDFAQPLPQNAAYDELAALLVPTHWPPNPAGEAA
                     LDRLLSQRGWPEMQRAISCGQYGHPEGLFYGGRNPTWSHVTLRQVLREQGRSCAQLGW
                     IDVHSGLGPWGVGERIFEGRAGDEAEGLRRARAWWGDQVTCIEDGESVSSSVTGQMMQ
                     ALYSECPQAPCTGITLEFGTVPLMEVVSALRAEQWLENHPESEGSIGREIRKRFRAAF
                     YPDSDSWRQQVLAQALEAARQAVAGLQTA"
     gene            complement(23995..24612)
                     /locus_tag="PKV56_04030"
     CDS             complement(23995..24612)
                     /locus_tag="PKV56_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOX67010.1"
                     /translation="MRVVWDFGGVLFRWDPAALLARTLPLRITAELSAQHWKEQFFLS
                     YRGEWGEFDRGVTTVSEMVPRISRRTGLSEAEVRAVVDAVPDELQPIDDSVRLLGALR
                     ERGHRLHYLSNMPAPLAHHLSHRHAFLKLFDSGIYSSHVKITKPDPAIFTLAQQRFGG
                     DPADTLFIDDHPENIEAALRHGWQARLFTGPQAMADDLRALGLID"
     gene            complement(24609..26234)
                     /gene="pgi"
                     /locus_tag="PKV56_04035"
     CDS             complement(24609..26234)
                     /gene="pgi"
                     /locus_tag="PKV56_04035"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOX67011.1"
                     /translation="MRCDRTVAWGALKGHFEAHGRDFDLREAFARDAGRFASLSFEAP
                     EVFADLSKNHLDLATLRFLVDLARECGLEAQRDAMFAGAAINTTENRAVLHTALRSPR
                     GRGPFSAQVHGVLDAMLAWAETVRDSATSRIRHVVNIGIGGSDLGPQMVVPALDAYVH
                     PGLQFHFVSNVDGHDITPVLRRLKPEETLFIVASKTFTTQETMANAHLARAWFEAEGG
                     KDIARHFVATTTNVKAAAEFGITTTFGFWDWVGGRFSLWSAIGLPIAIAIGADHFREL
                     LAGAHAMDRHFVEAPLASNVPALLGLLDVWYRNFHGAASRSIAPYHQGLRRLPAYLQQ
                     LEMESNGKRVDRAGELLPYATSPVVWGEPGTNGQHAYFQMLHQGTEAIPVEFILVRRP
                     TFGSSGLNADLLERLEMQQTMLLANGLAQSQALMRGKTAEQVLAEGSHPDAGALDAAT
                     LARHRSFPGNRPSTTLLLDRLTPHSLGALVALYEHRIFTSGAIWGINSFDQWGVELGK
                     ALCKDVQMRLLSGDLDGTALDGSTAGLITRLRG"
     gene            complement(26252..27199)
                     /gene="tal"
                     /locus_tag="PKV56_04040"
     CDS             complement(26252..27199)
                     /gene="tal"
                     /locus_tag="PKV56_04040"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOX67012.1"
                     /translation="MNQLDQLKRFTTVVADTGNFRQLAAFAPRDATTNPSLILKAVQQ
                     PDYAPLLTDTAASHRGVALDEIVDRILVRFGLEILKVVPGRVSTEVDARLSFDGPATL
                     ARARRLIALYEQAGIGRERVLIKIASTWEGIQAARVLEREGIHCNLTLLFSFAQAVAC
                     GEAGVQLISPFVGRIYDWYKKAAGAGWDEAANAGVNDPGVRSVAQIYRFYKKFGIATE
                     VMGASFRNVGQITALAGCDLLTISPELLAALQASEAPVARALDPAAARDAAIERVTHT
                     EASFRYALNEDAMATEKLAEGIRAFAVDAGKLDALIRAL"
     gene            27333..28787
                     /gene="zwf"
                     /locus_tag="PKV56_04045"
     CDS             27333..28787
                     /gene="zwf"
                     /locus_tag="PKV56_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOX67013.1"
                     /translation="MSFDLVFFGGTGDLTWRKLMPALFQAFRHGKLPPGGRILSVARD
                     EQSDEQYRGWLKERFQDVEGGKRPTDEEFSRFAELLHYRRMDLSQPEHYAGLKQWLDD
                     RGADTAVLYLATSPHLFTQICQQLGAVGLNGPKVRVVLEKPLGHDLASAQEINHVVRS
                     VFAETQAFRIDHYLGKPSVQNLMALRFGNALFEPLWRRESIANIQITLAESLGVGTRG
                     DFYDRTGTLRDMVQNHALQLLSMVAMEPPSRYDADAIRDEKLKVLRSLKPFTDDSVAR
                     DVVRGQYRAGNTAGQAVVGYLDEVKVPSGSRCETFVALRTEVQNWRWAGVPFYLRTGK
                     RLAARDAQIVVNFRPTPHSIFPGLNRPNKLVIKLQPEDGLELHLLAAKGTGQSEALSA
                     VSLDLDFDKAFPETRVGAYERLLLEAIAGRLNLFVRSDEQEQAWRWVEPVLHAWKRDN
                     DAGGGPRPYAAGTWGPAAASALVARDGFAWSEEQ"
     gene            complement(28833..29150)
                     /locus_tag="PKV56_04050"
     CDS             complement(28833..29150)
                     /locus_tag="PKV56_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX67014.1"
                     /translation="MTSSAAELALPPSVLLGDAKDLLKRLTRVVTENPSAPLIVDASS
                     LHDFDSSVLAVLLECRRLAQSSRREFVLRNAPPRLNQLAGLYGVAVLLGIEAPVKGAA
                     RLA"
     gene            complement(29147..29794)
                     /locus_tag="PKV56_04055"
     CDS             complement(29147..29794)
                     /locus_tag="PKV56_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX67015.1"
                     /translation="MQKKLVAAMCAGLLAGLVSPALHAEAPAVVAQAGATSPDDLINK
                     LSNEVLDTVKADKSIQSGDMKKIQALVEEKVIPHVNFQRMTSSAVGRYWRQATPEQQS
                     SLESEFKTLLMRTYAGALSQVKDQTVQIKPSRNAPEGNEVIVRTEVRGKGDPVQLDYR
                     VEKSNSSWKIYDVNVLGVWLVENYRNSFAQEIGANGIDGLIAKLTERNKTAAARK"
     gene            complement(29928..30662)
                     /locus_tag="PKV56_04060"
     CDS             complement(29928..30662)
                     /locus_tag="PKV56_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOX67016.1"
                     /translation="MTPTFRSGLKPLACIALAVALQGCATAPAGSAPSAGDPIESWNR
                     SVFAFNEAVDENAIKPVAKGYVNVVPSMVRTGVTNFFGNFKDAWSAVNSVLQGKVGNG
                     ISNAMRVGTNTVLGFGGLLDWATEFRIERFPEDFGQTLGVWGFGAGPYIVWPLLGPSS
                     VRDSFGLPLDMYVTPGFLIEDTEAGIGLAVLNVVNTRANLLNATGILDDAVLDKYTFV
                     RDGYLQRRNSLVYDGNPPQAPASQTK"
     gene            complement(30659..31153)
                     /gene="mlaD"
                     /locus_tag="PKV56_04065"
     CDS             complement(30659..31153)
                     /gene="mlaD"
                     /locus_tag="PKV56_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOX67017.1"
                     /translation="MQKSRHEVWVGLFVMIGAAALLFLALKAGNLLSLNFDSTYGVVA
                     RFDNIGGLKPRAAVKSAGVVVGRVESISFDDKTFQASVALQLQQKFQFPKDSSAKILT
                     SGLLGEQYVGIEPGGNEKNLAAGDVITQTQSAVVLENLISQFLFNKASEGAPAARATE
                     GEKQ"
     gene            complement(31185..31967)
                     /gene="mlaE"
                     /locus_tag="PKV56_04070"
     CDS             complement(31185..31967)
                     /gene="mlaE"
                     /locus_tag="PKV56_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HOX67018.1"
                     /translation="MNWLNPAWVGRTVLAQMAGIGAAAVLFVRLVALTPLALRRFTLV
                     IEQTFFVGNRSLSIIGVSGLFVGFVLALQGYYTLQRYGSAEAVGLLVALSLVRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQLAAMEMMAVDPVRRVLAPRFLAGLISMPLLAA
                     VFSAVGVLGGWAVAVLLIGIDEGAFWSQMQGGVSWWSDVGNGAIKSVVFGVTVTFVAL
                     WEGYDCKPTPEGVSRATTRTVVTSSLAVLALDFVLTAMMFSI"
     gene            complement(31964..32749)
                     /locus_tag="PKV56_04075"
     CDS             complement(31964..32749)
                     /locus_tag="PKV56_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67019.1"
                     /translation="MRGVTCGYGDRVILSNIDIDLPRGKVIGVMGTSGGGKTTLLRLI
                     GRQLAPMSGQVFFDGIDLAKLDRRGLYALRRRMGMLFQFGALFTDLSVFDNVAFPLRE
                     HTNLSPSMIRDVVLMKLDAVGLRGARNLMPSEVSGGMARRVALARAIALDPDLIMYDE
                     PFAGLDPISLGSASRLIRDLSQALGVTSVVVSHDVEETFAIADHIVFVANGVIAAQGT
                     PDEIRASTDPLVHQFFTASPDGPVEFHYPAASVTEDFGVGGRA"
     gene            complement(32926..34404)
                     /locus_tag="PKV56_04080"
     CDS             complement(32926..34404)
                     /locus_tag="PKV56_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOX67020.1"
                     /translation="MGKITGFMEYERLEEAYQPVQLRLKNYKEVAIGLKEGEARIQSA
                     RCMDCGTPFCNNGCPVNNIIPDFNELVFRGDWKNAIEVLHSTNNFPEFTGRICPAPCE
                     AACTLNVNDDAVGIKSIEHAIIDRAWAEGWVTPRPPKHRSGKSVAVVGSGPAGLAAAQ
                     QLARVGHDVTVFEKNDRVGGLLRYGIPDFKMEKLHIDRRVEQMQAEGVTFRTGVMVGR
                     LPEGSKVTNWAKEVISPEQLQAQFDVVLLAGGAEQSRDLPVPGRDLEGVHFAMEFLPQ
                     QNKVVAGDKLKGQLRADGKHVVVIGGGDTGSDCVGTSNRHGAASVTQFELLPMPPEHE
                     NKPLVWPYWPTKLRTSSSHEEGCDREFAIATKEFIGGEGKDKGKVKALKAVRVEWQGG
                     KMVEVPGSEQILPADLVLLAMGFVSPVASVLDAFGIDKDARGNARAGTEDVTGYATNL
                     PKVFAAGDVRRGQSLVVWAIREGRQAARAIDAHLMGRSDLPR"
     gene            complement(34494..>37203)
                     /locus_tag="PKV56_04085"
     CDS             complement(34494..>37203)
                     /locus_tag="PKV56_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOX67021.1"
                     /translation="FGMLAGYGAEAVHPYLAMETLANMHRDLPADLSTEKAIYNYIKA
                     IGKGLSKIMSKMGVSTYMSYCGAQLFEAIGLEKTMVDKYFRGTASQVGGIGVFQVAEE
                     ALRMHNAAFGEDPVLAGMLDAGGEYAWRVRGEEHMWTPDAIAKLQHSTRANQFETYKE
                     YAQLINDQSRRHMTLRGLFEFKLDPSKAVPLDEVEPAAEIVKRFATGAMSLGSISTEA
                     HSTLAVAMNRIGGKSNTGEGGEDPARYRNELKGIPIAAGTRISDVLGDKVIVADFELQ
                     AGDSLRSKIKQVASGRFGVTTEYLSSADQIQIKMAQGAKPGEGGQLPGGKVSEYIGFL
                     RYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANQRADISVKLVSEVGVGTIAAGVA
                     KAKADHVVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRSRIRVQADGQ
                     MKTGRDVVIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPALRAKFQG
                     KPEHVVNYFFFVAEEARRIMAQLGIRKFDDLIGRADLLDTKRGIEHWKAKGLDFARIF
                     HLPAAPAEVPRRQVEVQDHGLARALDVALIEKCKPALERGEKVQFMHEVRNVNRTVGA
                     MLSGELVRRHPEGLPDQTIFIQMEGTGGQSFGAFLAKGITFYLIGDANDYTGKGMSGG
                     RIAIRPSIEFRGDAMKNIIVGNTVLYGATSGEAFFRGVAGERFAVRLSGATAVVEGTG
                     DHGCEYMTGGTVVVLGETGRNFAAGMSGGVAYVYDPEGKFASRCNTSMVSLERVLSPA
                     EQGATDPALWHKGQTDDAILRKLIEDHHKWTGSLQARHLLDQWEASRAKFVKVFPNEY
                     KRALSELAAKEARARSVNTEADASATVVTSKAKASDKKTKASPSSK"
BASE COUNT         6643 a        12231 c        11962 g         6367 t
ORIGIN      
        1 cgcacggcac tgccggcctc gcgcgaggct caccgctacc tgatccagct gctcgtcgcg
       61 atgaaccggt cctccgagac cccggaaccg ctgcgctcac tcatcaagct gacctcggcc
      121 gctgaacggc cggggctgat cgccagcctg ccgcgcttct tcgagcgcac ctcggacagc
      181 aaggccgtgc cggagatcat cgaagccgca ctgaagagca gcgtcgacga gcccgaaacc
      241 cgcacggcat cgctggtatc gatcggccgc tcatggctca cagccggcaa caacgccaaa
      301 gcgctcgatt acgcccagcg tgcccatgtt caggacaaat cggcagaggg cccggtgctg
      361 ctcgctgtgg cggtgcttcc ggtggtggcc gaggcggaac ccgtcatcgt gggatatctg
      421 caagccaacc ctcgcgccag tgccatccgc atgatctatg cgcgcgctct cagcgtgtcc
      481 cagcggcacg ccgaagcgct ggctcaggtc gagacggtca cgcgtgacga acccgggcaa
      541 agcgcgccgt ggctgacact gggcgcgctg catctcgaac tgcggcagcc aaaggaagcc
      601 acggccgcgc tgaagacatt catcgaccgg gtgcaagccg cccccgcacc gacaccaccg
      661 tcgccagacg cggccgcggt accggctgag gaagaacagg tcgttgcgga tcccgatcgc
      721 gggctcacgc aagcttggct gatgctggcg caggcggccg aactccaggg cgacttcaag
      781 ggtgccgaag actggctgag caaggtgcag atgccgcagc gtgcactcga ggttcaggtg
      841 cgccgcgcat ccctgctggc caggcaggga cgcctggcgg aaggcgtgga gttgatccgg
      901 agcgcgcctg cgggcagcga ggacgaggct cgcgccaagg tgctagccga ggtccagctc
      961 ctgcgcgaag cgcgccagtg ggctcaggcg agcgcggtgc tgagcgaagc caaccagcga
     1021 tttgtcgacg atcccgatct gctctacgag caatcgatga tggaagagaa gctgaacaac
     1081 gtggcggaga tggagcgtct gcttcggcgc gtgatcgcgt tgcgacccga ccatcaccac
     1141 gcccacaacg cgctgggttt ctcgctggcc gatcgcggtc tgcgcctgcc cgaggcgaag
     1201 gcactgatcg agcacgcgct gcaactggcg cccggtgagc ctttcatcac cgacagcctc
     1261 ggctgggtgg aataccggat ggggaaccag gccgaagcga tcaagcagct gacccgcgcc
     1321 taccagtcgc gccccgaccc tgaaattgct gcgcacctgg gcgaggtgct gtgggtcagc
     1381 ggtcagcggg acgaggcacg gcgcgtgcta cgcgagggca cgcgtcgcga tcaggccaac
     1441 gatgtattgc gcgaaaccgt cacccggctg aaagtcgatt tgtgagattc ggggtatgcg
     1501 ctgctgcctg cctggcactg ttgactggct gcgcctccct gaaggcaccg gatgagccgg
     1561 cgcctgccga tctcctgacc gggaggatgt cggtgcaggt ggccgcaacc gaaaccactg
     1621 aagcgcacag catgtcggcg gccttcgagc tgagtggaac cccggaccgg ggacgcctcg
     1681 atctgaacac ccccatcggc agcaccgttg cgcgtgcacg ctggaaatcg gattcggtgg
     1741 tgctcgatac gccgcaaggc cagacccgcc acgcgacact ggccgacatg actcgcgccg
     1801 tggtcggtga ggaactgccg gtaccggcga tgttcgactg gctgcgcggt cggccctggc
     1861 cggcagccga aagcacccct tctatggcac cagccccgac ggggtttcgc cagttgggtt
     1921 ggaccgtcga cctgtcccga ttcgacgagg cgcgcatcct cgcgcgcagg gatgcgccgc
     1981 cggcggtgac ggttcaggtc aagctggaca agccgtgagt gcgccgcagc gtccccgccg
     2041 ccgcacggca acttgcagcg tggagcttgc ccgatgacgt tgcgcacgct gctcgacgtg
     2101 ccggcgccgg ccaagatcaa cctgttcctg cacgtggtcg gcaggcgcgc cgacggccat
     2161 cacctcctgc aatcggtgtt cgtgttgatc gactgggccg acacgctgca tttcgaacgc
     2221 cgcagcgatg cccgactggc ccgccatgac ctgacggaat cgctgcctgc cgacgacctg
     2281 tgcctgcgag cagcgcgcgc cttgcagtcg gccagcggca cggcctgggg tgccgacatc
     2341 agcatcacca agcaggtgcc ttggggcgca ggactcgggg gcggaagttc agacgccgca
     2401 accactttgc tggcgctgaa ccggctctgg gggctgaact ggtctcgatc gaagttgcac
     2461 gcattgggtc tggcgctggg tgccgacgtg ccctttttca taggcgggca aaacgcgtgg
     2521 gtcgagggaa tcggtgagca actcaggccg attgccgtgc ccatgcagac tttcatgctg
     2581 atcaagccac ctgtgtcgat cgagacacgt gcagttttta cccatcccgg gttggctcgc
     2641 gacactcccc gcgctatact tgagggcttt gatgcagacg catttcaaga ccttgtcgct
     2701 ccgaaagccg gagcaggctg gggtagaaat gacttgcagg tcgtggccga gtcgttgcac
     2761 cctgaggtgc atgaggcggt cagacagctg caagagcgct tcggcaacgg ccggatgagc
     2821 ggttctggga gcgcggtgtt cgcgcgcacc ggaacgagcg gggttgctgc tggagagctg
     2881 gagggcggtc ttcctgcggg ctggatcggc cgaaggtgtc gcagtctggt ggaacatcca
     2941 ctgcgcacct gggcagcgga ttgagagatg aaaggttttg ggtacgcggc ttcggcggcg
     3001 taccggtgta ggggagtcgc caagttggtt aaggcatcgg attttgattc cgacatgcga
     3061 aggttcgaat ccttcttccc ctgccagatt cattggattc ccggaacgtc ctgacaacag
     3121 gcctgggctg gactgaaagg aagcgtcatg cttccacaga gcctcgttgg agggaatgtg
     3181 cttttcaaca ccgtgctgtt cactggcaac gccaatccgg tgcttgccca ggaaattgca
     3241 acccatctgg gcgtcgaact gggcaaggcc tcggtcgggc gattctccga cagcgaggtg
     3301 gctgtcgaga tcgaacagaa cgtgcgggct cgcgacgtgt tcgtggtgca gtcgacctgc
     3361 accccgacca atgagaacct gatggaactg tgcatcatgg tcgatgcatt gaagcgcgcc
     3421 agtgcgcgcc gcatcaccgc cgtgattccc tatttcggct atgcccggca ggatcgccgg
     3481 ccccgctcga cacgcgtgcc gatcagcgcc aaggtggtcg ccaacatgct ggaagccgtc
     3541 ggcgtcgaac gactgctgac catggacctg cacgcagacc agatccaggg cttcttcaac
     3601 atcccggtcg acaacatcta tgcgtcaccg gtgctgctgt ccgacctgca atcgaagcag
     3661 tacgaggatc tggtcgtggt ctcgcccgac atcggcggcg tggtgcgcgc gcgcgccctg
     3721 gccaagcaac tgaactgcga tctggcgatc atcgacaagc gccggcccaa ggccaatgtg
     3781 tcggaagtca tgaacgtcat cggcgaaacc gaagggcgca actgcgtgat catggacgac
     3841 atgatcgaca ccgccggcac actggtcaaa gcggccgacg tgctcaaggc gcgcggcgcg
     3901 aaaagcgtct acgcctattg cacgcacgcg gtgttttccg gcccggccat cgaacgcata
     3961 cgctcctcga tgctcgacga agtcgtgatc accaacacca ttccgatgac cgacgccgcc
     4021 aaggtgtgcc cgaaggtgcg ccagctgtcg gtggcattcc tgtttgccga aacgatccgc
     4081 cgtatttccg acggagagtc ggtcacttct ttgtttgccg agcagaacag caatttctga
     4141 tcgtcgaaca ctgcgtccgg ccagccagcc gggcgtccta ccgggtcgaa aggcctgttt
     4201 cgaagcacct ggtcgcgggg cttcaccact ttgggagtca acatgaaatt cgtcgcatac
     4261 gagcgtacgt tgcaggggac cggagcgagc cgccgcctgc gcaacgctga caaggtacca
     4321 ggcatcgtct atggcgcggg tacaccggcc aagatcgaac tcgatcacaa cgcgctcttc
     4381 ttcgccttga agaaggaagc cttccactcg tccatcctcg agatggaact ggcaggcaag
     4441 accgagcagg tcctgctgcg cgactatcag ttccacccgt tccggccgat cgtgctgcac
     4501 gtcgatttcc agcgtgtcga tgccacgacc aagatcacga agaaagtgcc tctgcacttc
     4561 atcaacgagg atgagtcccc ggcggtgaag gtcgacaagt gcgtcgtcaa ccacgtcgtg
     4621 accgagttgc gcattcagtg cctagcatcg cagttgcccg agttcttgac ggtggatctg
     4681 ggtcagctgg tcaagggcca atccctgcac gtcaatgaca tctccctgcc tgcaggcatc
     4741 aaggtcatga cgcagggcaa gccgaatccg gtgctggtgt ctgcctcgat gctgacagcc
     4801 gaggaagaag ccgctcccgt ggtcgtgacc gctgccgcag cagcgccgac caagggcaag
     4861 aagaccgaga agcagaacaa gaagtaagcc ggggtccgct tccgctcccc cttcggagcc
     4921 ccaccacggt ggggcttttt catgggcgcc tcaattcgat aatcgaagca tgattcgact
     4981 catggtgggt ctcggcaatc cggggcagga atacgaagga acgcggcaca acgccgggtt
     5041 ctggttcatc gacgaggtgg ctcgacacct caaggtgacc ctggccccgg acaggtcgta
     5101 tttcgggctg gtcgcacgcg tgaacctgcc aggcggcccg ctgtggctgc tcgagccgat
     5161 gaccttcatg aatctgtcgg gcaaatcggt ggcaccactg gcgcgcttct tcaagatcga
     5221 gccggaggac atcctggtcg cccatgacga gctcgatctg atgccaggtc agatgaagat
     5281 gaagctcggt ggcagccacg ccggtcacaa cggactgaag gacattcagg cccagctcgg
     5341 ctcacccggc ttctggcgcc tgcggcttgg catcgggcac cccggagtca aggctgaagt
     5401 tgtcaaccac gtgctgcgca aaccgcccct ggcggagcgg caggccattg accagagcat
     5461 cgcgcaggcc ttgggcgccc tcgatctgat gattgctggc gacatggagc gggcgatggt
     5521 gaagatccat gctcgtccgc cacggccgaa gaagcctccc gaatccaacg aaaggcccgc
     5581 atcatgaagt tgctcgcgat gtcttgtgcc ctgttgctga cagcggtcgc cagttgggcg
     5641 gcggctggcg aaatctatcg ctgcggcccg gccggggccg agttttcgca ggctccctgc
     5701 gcgaatggtg cgcgcttcga ggtgtctgat tcccgcaccg ctgaccagta ttcgcaggcg
     5761 gtggtgctcg cagcgaaatt cgaggcttgg ggctcggcga tggagcgcga tcggctggcg
     5821 accgaggcct cttatcgtcc caccttggcg gtcagcttga attcatcggc aagcggcaca
     5881 accgagtacc ggcctcggtc ggcctcccag cccaaacgca agcgtgtcaa ggccgtgtcg
     5941 gctggcgttc cgctgttgtc acacggtcgg cccaggttcg accgggatcc agatgccgac
     6001 ttcagtcggc gatagcactg gcggtgccat tcgaagcctc ggtgcgagcc gcggcctgaa
     6061 gtcgccggta cttgatccac aacgattcat tggactcgac gtggtctgga tggacaggga
     6121 tgcaattcac cgggcagacc tgcacgcact gcggctcatc gaagtgcccc acgcattcag
     6181 tgcagcgcgc cggatcgatc tggtagatct cgatgcccat tgaaattgcc gagttcgggc
     6241 actcgggctc gcacacgtcg caattgatgc attcgtcggt gatcagcagt gccatgtcgt
     6301 gagtgtcgag cgttcaggct tcggtgtact tgactcgcga gcgcaaccgg tcgagcaccg
     6361 acggtgacac aaatttggaa acgtcgccgc cgagtgaagc gatctccctg acaaaggtgc
     6421 cggagacgaa ctgatactga tcactcggag tcaggaacac cgtctcgaca tcgggcatca
     6481 gctggcggtt catcccggcc atctggaatt catactcgaa atcgctgacc gcgcgcagac
     6541 cgcgcacgac gaccttgccg ccgatgccga cgacgaagtc gcgcagcagg ccgcggaatg
     6601 caatcacctc gacgttggga tgcttgcggg ccagttcctc ggcgatgtcg aggcgctctt
     6661 cgatggtgaa catcgtgcgc ttgtggtggc cggcagccac cgccacgatc agtcgctcga
     6721 acagccgact ggcgcgacgc atcaggtcct cgtggccgag tgtcatcgga tcgaaggtgc
     6781 cgggatagat tgccgtcagt ttggtcacgg aagtcattcg atcccccctg tcacgcggtg
     6841 atggtcgcag tgtagtcagc cgcggcacgc tgcagcaggt gcgcatggac caggccggct
     6901 cggaaatgtc ggtgcaccgt cagcccgagc ggtgcgactt cctccccgct gtaggcgtgc
     6961 gctgcttcca ggtagatgta accctgcggc acaaccaggc gagcagccgc agcgagggcc
     7021 gggcccagca gatcgctgcc gaagggcggg tcgatgaaca ccagttcgaa tgaatcgggc
     7081 acgcaacggc gcatccacga caacgcgtcg gtggcttcga tgcgcagcat gtcggccttc
     7141 aggcgcagct tcgattcacc cagactgcgc acctgcctct gttcgcgctc gagcatcacc
     7201 acggacgcag cgccgcgcga ggcggcctcg aatccgagcg caccgctgcc gccaaaggca
     7261 tcgagacagc gccacccact cagatcggca cccagccagt tgaagagcgt ctcgcgtacg
     7321 cgatccggcg tgggtcgcag gccgggcaac aggatgacgg gcagcttgct gcgcttccat
     7381 acgccgccga tgatgcggac ctcgcggggc agcgatgccg tatcgggcgc gaccggcggc
     7441 ttgtccgccg ccttcttgtg cgccttcttg acggaaggcg actccgtgta ggcacgactg
     7501 ccaaaccgga ggctggcttt ggggtcggct ttcgcggcaa ccttggcggc agctttcggc
     7561 ctgggcaccg atttgctgat gatcgacgcc atcacagccg caccggaatg ccgtcacgca
     7621 ccatcagcgc cttcgcctgg ccgacagtga actcgccgta atggaagatg ctggctgcca
     7681 gcaccgcgtc ggcaccaccg acacgtatgc cctcggacag gtgctccagc gcgccgacgc
     7741 cgccggaggc gatgacggga acctcgaccc tgtcgctgac tgctcgcgtc agcggcaggt
     7801 cgaatccgga cttcgtgccg tcgcgatcca tgcttgtcag caggatctcg ccggcgccat
     7861 gctcggtcat ctgcgccgcc cagtcgaccg catcgagacc ggtgttcttg cggccaccat
     7921 gggtatagac gtcccacccc gggcctcgca gggccaggtc atcgccgacc cgacgcttcg
     7981 cgtcgatcgc cacgacgatg cattgcgcgc catagcgacc tgatgcgtcg cggatgagtt
     8041 gcggattcgc gaccgcggct gaattgaagc tgaccttgtc ggcacctgcg ttgagcaggc
     8101 gacgcacatc ctcgaccgaa cgcacgccgc ctccgaccgt cagcgggatg aacacctgcg
     8161 atgccaccgc ctcgatgatg tgcaggatca gatcacggcc gtcgctggtg gcggtgatgt
     8221 cgaggaaggt cagttcgtcg gcaccctggt cgttgtagcg cgcagcgatc tccaccggat
     8281 ccccggcgtc acgcaattcg acgaagttga cgcctttgac gacacggccg ccggtcacgt
     8341 cgaggcaggg gatgatgcgc ttgcaaagca tgtgcggtcg ccagctgcca tcgcagggcg
     8401 tcgagagtgt gaaagatgcg gggtcaggtc ccgctcagct cgtcagctcg cgtctgggcc
     8461 gcagcgaaat ccaggtcacc cgagtagatc gcccgcccgc agatcacgcc atcaatgcct
     8521 tcgaactcga ccgcgcacag gcgatcgata tcggccaggt tgctcagacc gcccgaagcg
     8581 atgaccggga tggtcaacgc ctgcgccagc ttgaccgtcg cttcgatgtt gatgccgctg
     8641 agcatgccgt cgcggccgat gtcggtgtag atcacgcctt cgacgccgta gtcctcgaac
     8701 ttcttcgcca gatcgacgac ctcgtggccc gtgagcttgc tccacccgtc ggtcgcgacc
     8761 ttgccgtcct tcgcgtcaag cccgacgatg atgtgacctc cgaaggcgct gcacgcatct
     8821 cggaggaagc cggggttctt gaccgctgcc gtgccgatga tgatgaagct caggccgtcg
     8881 tcgaggtagc gctcgatcgt gtcgagatca cggatgccgc caccgagttg caccggaatc
     8941 tcgtcgccca cctcggcgag gatggccttg atggccgatt cgttgaccgg ctttcctgca
     9001 aaggcaccgt tcaggtcgac cagatgcagt cgccgcgcac cggcgtcgac ccagcgccgc
     9061 gccatcgcag ccggatcctc cccgaacgtc gtcgagtcgt tcatgtcgcc ttgcttcaag
     9121 cgcacacatt tgccgtcttt gaggtcgatc gcgggaatca gcagcatggc cggggcgaac
     9181 agttgaaaaa acagattatt tcggaggcag cgatgactgc agccggttga aggggacaac
     9241 ccctgaagag tgcctgatat cggcgctcat ggattccaat ggaggaaatt tcggtacaca
     9301 gccaaaccat gcagggcgct cttttccgga tggaattgcg tcgcaaaaat attatcccgc
     9361 cccagagcgc aggtaaagcg ctgcccgtag tcggtttcgc cgatgctgtg ggcacgatct
     9421 gcgggacgcg cgtagaagct gtgcacgaag tagaagctcg ccccgtcggg cacgccagcc
     9481 cagagtgagt gcgggcggac ctggatcacc tggttccagc ccatctgcgg caccttgtag
     9541 cggctgccgt caggctgcag ctgcccgtcg agccggaacc ggatcacatc accctgcatc
     9601 agcccgagcc cggccgtgcc ctcgcccggc ggtccctcct cgctgtgcga aagcagcatc
     9661 tgcatgccga cacacacacc gagcaacggc ttgcgggctg ctgcatccag cacggcctcc
     9721 ttcaagccgg aatcgcgcag ctcgcgcatg cagtcgggca tcgcaccctg ccccggcagc
     9781 acgacgcgct cggcggcata aacgacatcg gggtcggctg tcaccagcac ctcgaaaccc
     9841 gagccctgcg ctacgtgcat cacggcctgc gacaccgagc gcaggttgcc catgccgtag
     9901 tcgaccacgg caaccgtctt ccccatgatc agagactgcc tttggtcgac ggaatcacgt
     9961 tgcccatgcg cgggtcgatt tcaagtgcca tgcgcatcgc acgggcgaac gccttgaaga
    10021 tcgtctcgca ctgatggtgc gcgttgtcgc cgtgcaggtt gtcgatgtgc aggctgacgc
    10081 cggcgtggtt gacgaagccc tggaagaact cgtagaccag ttgcgtgtcg agcgcaccga
    10141 tcatgccgct cttgaagtcg acgcgcatgt gcaggccggg acgaccggaa aaatcgatca
    10201 cgactcgcga caaagcctcg tcgagcggca cgtaggcatg cccgtagcgg cgcaggccgc
    10261 gcttgtcgcc gaccgccgtc gccaccgcct ggcccagagt gatgccgatg tcctccacgg
    10321 tgtggtggcc atcgatgtgc agatcgccca cggcctcgac gttcaggtcg atcaagccat
    10381 gacgcgcgat ctggtcgagc atgtgatcga ggaagccgat tccggtggcg aactgcccca
    10441 cgccggtgcc gtcgaggtcg aggcgaacgc gaatctgcgt ttcagcggta ttgcgcgcca
    10501 cttccgcgcg gcggtgtgac aaggccgggg ccgtggtgtc tgggctcatc gcggactcgc
    10561 ttcaagggag gacttcaagg cggataacag gatgtcattc tcttccggcg tgccgaccgt
    10621 gaggcgcagg caatcggcca gcagggggtg cagattcgag acattcttga cgagcacgcc
    10681 gcgattcttg agtgcaccga acaccgccgc cgcatcgttc acgcgagtca ggatcatgtt
    10741 ggcttcgctc ggatagggat gcacaccggg caaggcgtgc agtgccgaag tcagccgcga
    10801 tcgctcgctg cgcagcaccg cagcctgccg gtcgaactcg tccgcgtgtt ccagcgcgaa
    10861 catcgcggtc tcgcagttga gcacgctgac gttgtagggt ggcctcacct tgtcgatctc
    10921 ggcaatcagt gccttcgggc ccatcaggta accgagccgc acccccgcca gcccgaactt
    10981 gctcagcgtg cgcatgatca gcacatggcc atggcgagcc acgcggtcga tgtagctgcg
    11041 cgaggcaaac ggctgataag cctcgtcgat cacgaccagg cctggcgcgg cctcgatgac
    11101 gcgctcgatg atcgcgtcat cgaagaggtt cgccgtcggg ttgttcgggt aggcgatgta
    11161 gacgatcgcg ggctgatgct gctgcatggc agcaagcatc gccgactcgt cgagttcgaa
    11221 gtcgggcctc agcggcacac tgatcgagcg caagccctgc agccgcgccg acacgtcgta
    11281 catcacgaaa cctggcaccg gggcaagcgc gctggctccc ggggttgcac acgccagact
    11341 cagcatcgag atcagttcat cggatccgtt gccgaggatc aggtcgaagc cctcgggcaa
    11401 gccggcgtgc tgcgcaagcg cacaacgaag atcgttgttt cgactgcccg gatagcggtt
    11461 gatcgcgacc tcggccagac gcgcaccaag tgcggcgcgc agcggttcgg gcaagcgaaa
    11521 cgggttttcc atcgcatcga gcttcaccag tccgtcgctc ggctgcaccg cgtaggcgag
    11581 catcgcccgc acatcgcctc ggatgtgccg ctggatcacg tcggacgtcg tcggagggct
    11641 catcaagaat cgctgtcgcg gcgcttcatg cgcatttcgg ccgcctgggc gtgcgcctgc
    11701 aggccctcgc cgtaggcgag ttcggcagcg acgccaccga gtttctgcgc cccggcctca
    11761 ctgacctcga tcaggctgct gcgcttctgg aaatcgtaga cccccagcgg cgacgagaag
    11821 cgcgctgtgc ccgaggtcgg cagcacgtgg ttcggcccgg cacagtagtc gcccagactc
    11881 tcgctggtgt aggccccgag gaagatcgcc cctgcgtgcc tcagcagcgg ctcccatcgg
    11941 tgcggatctc gcgagctgac ctcgaggtgt tcgggcgcga tgcggttgct gatctcgcag
    12001 gcctcttcca tgctgcgcgt gcggatcaag gcgccgcggc cttcgagcga agcgcggatc
    12061 acgtcgcgtc gcggcatgcc ttcgatcagg cgatcgatct cggcctgcac gcgctcgatg
    12121 taggccgcgt caggacacag caggatgctc tgcgcgagtt cgtcgtgctc ggcctggctg
    12181 aacaagtcca tcgcaaccca ctcggccggc gtgctcccgt cggccagcac caggatctca
    12241 ctcggtccgg cgatcatgtc gatgccgacc ttgccgaaca cacggcgctt ggcactcgcg
    12301 acataggcgt tgcccggacc ggtgatcttg tcgactgccg gcaccgtggc cgtgccgtaa
    12361 gccagcgccg caaccgcctg tgatccgccg atcgtgaaga cgcgatcagc gccggcaact
    12421 gcagcagcag cgagaaccag caggttcttc tcgccccgcg gcgtgggaac aaccatcacg
    12481 atctcgccga cgccggccac cttggcgggc accgcattca tcagcacgct ggacggatat
    12541 gccgccttgc cgcctggaac atagatgccg acccggtcga gcggcgtgac cttctgaccc
    12601 agcaaggtgc cgtctgcatc gacgtaggac catgagcggc cacaggcttc gagctggcgc
    12661 tggtggtagt cgcgcacccg atccgcggcg aactcgagcg cgctgcgctg ggccggcgtg
    12721 atggcggcaa gcgcggcgtg caactcgtcg tgcccgatct ccagcgcggc caccgatgcc
    12781 gcctcgacgc catcgaagcg ccgggtgtac tcgagcaccg ctgcatctcc gcgaagctgc
    12841 acatcggcca ggatggaggc cacccgctgc tcgattgcat cgtcggtctc ggccgaccag
    12901 tgcagcacgc gctcgaactc ggcggcgaag cctgctgcgg aagtgtcgag gtggcggatg
    12961 cgaacggtca tggtggaaca gctcagcttt gaatcgcgga agcaaacgcg tcgatgatcg
    13021 cgcggatcgg ttcgcgcttc agcttcaacg cagcctggtt gacgacgaga cgggccgaga
    13081 tctgcatgat ctcctcgacc tcgacaagat gattggcctt caaggtgttg ccgctggaca
    13141 ccagatcgac gatcgcgtcg gccagcccgg tcagcggagc cagttccatc gagccataga
    13201 gcttgatcag gtcgacatgc acgcccttgt cggagaagtg ctgctgcgcc atcagcgtgt
    13261 acttggtcgc cacgcggatg cgcgaaccct gcttcaccgc actggcgtaa tcgaagtcgg
    13321 cacgggtggc cacgctcatg cggcacttcg cgatcttcag gtcgaggggc tggtacaggc
    13381 ccgaaccgcc gtgctcgaac agcgtgtcct tgccggtcac acccaggtcg gctccgccgt
    13441 actgcacata ggtcggcaca tcgctggcac gaaccagaac gacacgcacg tcggctcggt
    13501 tggttccgat gatgagcttg cgcgactttt ccggatcttc ggaaacctcg atgccagccg
    13561 cctgcagcag cggcatggtt tcttcgaaga tgcgtccttt ggacagtgcg agcgtgatca
    13621 cgtgttggcc ttcattgaac gcgctcgatg tcggcgccga tgccgcgcag cttggcttcc
    13681 atccggtcgt agccgcggtc gaggtggtag atgcgatcga cacaggtctg gccgtccgcc
    13741 acgaggccgg cgatcaccag gctggccgat gcgcgcaggt ccgtcgccat caccgtcgca
    13801 ccggacaatt tctggacgcc atgcacaacc gccaggtgac cgtcgatgtc gatgcgcgcg
    13861 cccagccgca ccagttcatt cacgtgcatg aatcggttct cgaagatggt ctcggtcacc
    13921 ttggcggtgc cgtcggcgat gcagttcagc gccatgaact gcgcctgcat gtcggtcgga
    13981 aacgccgggt actcgctggt gcggaaactc acggcccgcg gccgctgctg gctgcgcacc
    14041 cggatccagt cctctcccga ctcgatcgtc acaccggcct cgcgcaattt gtcgatgacc
    14101 gcgtcgaggt ggtcggcgcg cgcacggcgc agcaccacat cgccgccagt cgcagcgact
    14161 gcacaaagga aagtgcctgc ctcaatgcgg tcgggaacga tccggtggct ggcagccccg
    14221 gacaatccgt gcaggcgatc gacgccatgc acccggattc ggcgcgttcc gtgtccctcg
    14281 atgcgggcac ccatcgcgat cagcatgtcg cccagatccg ccacctcggg ctcctgcgcc
    14341 gcgttctcga gaaccgtctc gccttcggcc agcgtcgcag ccatcagcag gttctcggtg
    14401 ccggtcacgg tgaccatgtc ggtggtgatg cgcgcgcctt gcaggcgggg tgtcttcgcg
    14461 atgatgtaac cgtgctcgac agtgatctgc gcgcccatcg cctgcagtcc cttgatgtgc
    14521 tgatccacgg gccgcgagcc gatcgcacag ccgccgggca gcgacacagt ggcctcgccg
    14581 aagcgcgcca gcagcgggcc cagcaccagg atcgaggcgc gcatcgtctt gaccaggtcg
    14641 taaggcgctt cgggcgaggt caccgtggac gtgtcgagcg tgacggcatc gggctgtatt
    14701 tcgctgcgct cggcagtcgc acccatgttg cgcagcagct tgagcgtcgt gctcacatcc
    14761 tgaaggcgcg gcacgttggt cagcgtcacc ggctcggcgg tcagcaacgc agcgcacaac
    14821 tcgggcagcg ccgcattctt ggcgcccgag atcgtcacct cgccttcaag aggccgtccg
    14881 ccgcgtatca gaagtttgtc catgagcgtg agagcgaaac ctgcactgcc ttcgaacccg
    14941 ccgggacgga ttcaacagcc gcagcaagcg ccgcggcggc tttcagtgat gttgcagtgg
    15001 agctttgtcg gaactggcag cccactcggc gggcgtcagc gtcttcatcg acagcgcatg
    15061 gatctcggcg cgcatcttgt cgcccagcgc gcggtacacg agctgatggc gtgccacacg
    15121 gctggcacct tcgaatgcgg cgcagacgat ggtcgcgaag aagtgctggc cgtcgcccag
    15181 cacctccaga tgctcgcagg cgagaccttg cgcgatgtag cgctgcacgt cgttgggcgt
    15241 ggggtcagac atcggcgaat tatggctcag cctgctcaat ggcgtatctt gtagccggtt
    15301 cgcagcaagt gcaggcacag gccgcacacc gccagaagac ctgcgccggc cacgcaaagg
    15361 ctgacccagg gcgacacgtc gctgacgccg aagaaaccga accggaaacc atcgacgatg
    15421 tagaagaacg ggttcagatg actcgcctcc tgccagatcg ccggcagact cgagaccgag
    15481 taaaaaacac cggccaggaa cgtcatcggc atgatcagga aattctggaa ggcagcgatc
    15541 tggtcgaact tctccgccca gagaccggcg atcaagccca gggcacccat caatatcgca
    15601 cccagcagag tgaaaacgac gatccaggcg ggctgtgcca gctgcaaggg agcgaaccag
    15661 gccgtgacag ccagcacgcc ggtgccgacc gccaccccac gcacgaccga ggcccccacg
    15721 taggaaacaa accaggccca gggcgaaagc ggcgcgacca gcaggaatac caggttgccg
    15781 ctgatcttgc tctggatcag cgacgagctg ctgttggcga atgcattctg gagaacgctc
    15841 atcatcacca ggcccgggat caggaaactg gtgtagccca cgccgctgag cggctgcacg
    15901 ttctcctcga gtacatggcc gaagatcatc aggtacagca cggcggtgag cacgggtgcg
    15961 gccacggtct ggaagctgac cttcaggaac cgcagcacct ccttgtagaa cagggtgcgg
    16021 gcacctgaca gccgatgtgc actcgtcgcg ctggccggag ttgtcatgcc gccccctgca
    16081 acggttcgcc ctgcatgatt tccaggaaca cgtcctcgag gtcggcgcgg ccgatctcga
    16141 ggtcctccgg ttgagcgcca gcggagcgca gcgtggcaag caccttctcg acctcggccg
    16201 cgttgcgcgt cttgatctgc acgatgcggc cggtgacgcg ggcctgggcg agcagcgcgg
    16261 gtggcagcgg tgtgtcgagc ttgaaccgca gcatcgtgct ggcgcgcccc gcgagcaggg
    16321 cagaggtacg atcgagcgcc accacacggc cctgcttgag catggccagg cggctgcaca
    16381 gcgcctcggc ctcctcgagg tagtgagtgg tcagcagtac cgtgtggccc tccttgttga
    16441 ggcgcgcgat gaactgccac agcgtctgcc gcaactcgac gtcgacccct gcggtgggtt
    16501 catcgagcac gatcaccggc ggccggtgca ccagcgcctg tgccaccagc acacggcgct
    16561 tcatgccgcc ggacaactga cgcatgttgg caccggcctt gtccgtcagg cccaggtgat
    16621 cgagcagttc gtcgatccag gcgtcattgg cgcggcggtc ggtgcgcact ccgaaatagc
    16681 cactttgaat ctgcagcgtc tcgcgcacgg tgaagaacgg atcgaatacc agttcctgcg
    16741 gcacgatacc caggcttcgg cgggcagcgg catagtcggc aacgacatcg tgcccatgca
    16801 ccaggacctg ccctgcgctg gcctgcgcca ggcccgcaag gatgttgatc agcgtggtct
    16861 tgccggcccc gttgggcccg agcaggccga agaactcacc gggggcgatg tcgaaggtga
    16921 ctccgtccag cgcctgcacg gtcccccgag aacccctgaa acgcttgctg acgtcgcgaa
    16981 acgagacggc agcaatggac gagccaggga cgagatcaga catgaagcac gccattgtag
    17041 gcagcgccct tccacgacag tgcggcgcca gggcagcaac ggcgcgggga agaccgtcgc
    17101 cggatacccg ggcagatgcg cttggacgcg cgccgagttg gtgctacctt gattgcacgg
    17161 ttcatcgata cgaaagcagg aaccatggcg acacccgtca agaagccacg ccgaacggcc
    17221 gagcgcatcc tcgatgtgac actggagttg ttcaaccgtt tcggcgagcc gaacgtctcg
    17281 accacgctga tctcggccga gttgaacatc agcacgggca acctctacta ccactacccg
    17341 gcaaaggatc tactcgtcga ccggctgttc gaccgctacg aacaggcgct gggttcgctg
    17401 ctgaaggcag ccgacgacgt ccgcgatgtg gaggatgcgt ggctcttctt ccacatgctt
    17461 ttcgagctga tctggcagta ccggttcctg taccgcgacc tgaacgacct gctcagcaag
    17521 aaccggcgga tcgagaccca tttccagttc gtgctgcagg aaaagaatca ggccgtccag
    17581 agcgtgctgg ccgggcttgg acgcagcaac gccatccggc tcacggcggc agaggccgaa
    17641 tcggtggcga ccgcgatggt cgtggtggcg acctactggc tcagctacga gtacgtgcgc
    17701 gacccgaggc acgcgctgga gcccgagaac gcaggggcgg ccctgctgcg cggagcgtcg
    17761 aatgtactga gcctgctggc accgtacttc gaggcggccc agaagcagca cttgctgggc
    17821 cttgcgcagg cgtattcgaa accggcagcg gcacccgttc tggaggcggc ggcccgccgt
    17881 ggcaccacgc aggccgcgac ttcggcccgg gcagcgctgt agcgtcagcc cttggactgc
    17941 ggagctgctg cagcacctgc ctgtgcgatc gccgcttcga gattcctctc gatctcgtcg
    18001 cgtatgttct gactgaacgc cgacaacagg aaaccgagcg tcaccgtcag gtcgaagcag
    18061 tcggcgctgg caacgagttc gccgcgcact ccggaccgag cgaaatcgac ccggttgtcc
    18121 acctcgcctt cgcgaaccgt gcagttcatc tgatgcttct gctgggcggc ttcggcccag
    18181 tgccgtgcca ggtcccgagc cggttcgaga cccagggagt gcggtcggtg gatttggaag
    18241 gtggtcatgt ggtcagcgca gtttaggtac gcggttcggt ccgttcaatg aggagacgcc
    18301 cgcagcagga cctgccggcg ttcagccatc ggcagcgcgg ccagacgccg cacctctgcg
    18361 tagaagcgag gcagatcgcc gccctgtgat tcgagcagct gctcgaactg cggcaccagt
    18421 tcgttgtagg cagccagcac gccgaacgac gcattgttga cctgtgcgaa ccagccgtcg
    18481 tagccgctgt aaccctgcca cgctccagcc ttcagcgcgg cgtgttcggc gcgcaactgg
    18541 gccagcagtt cggtcttgcc gcgtcgcttg tcgctgtctg gcgcggcacc cgcgtagagc
    18601 gcctgaaggc ggtcgcggta cctgccggtc aacgcacgga aatcggcgcg ccgcgactcg
    18661 aaggcggcga actcccggcg cgctgcgtcg ccggcacgcc gggacagcca acgggcactg
    18721 ccaaggcgtt cgaccgcggt ggcgaacgat tcattgaaca cggtgtcgtc actggcgtgg
    18781 gccacctggt gcgacagctc gtggaagatc aggcgcgcca gttcgccgtc cggatagtgc
    18841 acgaaggtgt tgagcagcgg atcagcgaag tacgcaccag gcaacttgcc cagcgtcgaa
    18901 tacgccggca cgccgtacac ccaggcctcg agcccgcgcg cacgaagctg ttcggcgtag
    18961 gcgtcggcct cttctcgggc gtagtagccg cggtacgcaa cgcagccgac gaccggaaag
    19021 caccaggttt ccagcgtcag acccagttcg ggtgcagcca ccacattcca caccgccgca
    19081 gcgcgctgca ggtcggcata gcgccggtag ctggcgttgt cgggcagatc cagttcggtc
    19141 accgcgaaat cgcggatctg ccgggtcagc tcgagccgtg ccttgagctt gtcgggcgtc
    19201 gccgggtcgg ccagccactc aggcacaggt cgcgcggcgg caagcagatt gaggtgcccg
    19261 ccgatcgact gcgcgtagta gcccacgctg ctgcagccgg tcgccaagcc cgatacggcg
    19321 gcgctgagca gcacggcacg gatcagcgcg ccagacttca agcggcttcg acctgcacca
    19381 ggcagtcgta gaacgccggc ccgcggccga tgtcggtcgg caactggtgc gtgacctcgt
    19441 tgacgttggt tccgcccggc ccgtccttgc gccaccagat gcccaggcca ttgacgacgc
    19501 caggacgcgc ccgtgcgctg atggccgtgc ggcacaggta gctgccgcga tcattgaaga
    19561 cgcgcaccgg ctgtccctct gccagaccac gcgccgccgc gtcggcggca tggatctcaa
    19621 gcagcggctc gccttcgatg tcgcgaaggc tgcgcaggtt gacgaaggtg gagttgagga
    19681 agttgcgggc gggcggagag atcatcgcca gcggataacg acgcgccagc gccggcgcac
    19741 tggccaacga ttcgtggttc gcgacgtggt cggccatgcc gtgcttgatc gaactgatca
    19801 tgcagcggcc gctggcggtc gggaagcctc cctgggcgaa cggtgcgtcg gcgatcggca
    19861 gcttgatcca gcctcgggcc cgcaactctt cgaagggcac cacggccgtg aacgcgccac
    19921 gcgccagggc ctcgtcatca tcggcaaagc aggcgtcgtc ccagcccatg cgcgctgcca
    19981 gctcgcgaaa gatctgcgtg ttgggccggg cttctccctg cggctcgatc gcacgctcgt
    20041 tgatcagcgc gtaggtatga ccgtaactgg cgtgcacatc caggtgttcg agctgcgtcg
    20101 tggccggcag cacatagtcg gccaggtcgg cggtgtcggt catgaagtgt tcgagaacga
    20161 cggtgaacag atcctcgcgt gcgaatccgg cgaccacctt gggcgactgg ggcgccacgg
    20221 caaccgggtt gctgttgtag acgacgacgg cctcgatgcg cgggccgaaa tccgtgccat
    20281 cgggcagcac ctcacctgat gggcgcagca ggtcgtcacc gatcgtgctc atgttcaggg
    20341 tgcgcggcat tgcacggcgg ccatcgggcc cgccgagcag atccggccgc tgcaaccacg
    20401 cgtcgttgcg ggcaccccgg aaccagccgg agctcgacag aagcaagccg cctgcgcggt
    20461 ggcgccaggc accgatcagg cagggcagca tcgcgatcag gcgcaccgcg ctgccaccac
    20521 cgcgcacccg ctgcatgccg tagttcaggc gaatcgcggc cggggccatc gaggcgcaat
    20581 cgcgggccag cgatctcacc tcgtcggcag tgatgccgca gaccccggcc acgcgcgcgg
    20641 gcggccatga catggcacgc tcgcgcagcg cgggccaccc tccctcgccc tcctcgacgt
    20701 gacgttcgat gtagtcgtga tccaggcgat cgtgcacgat cagttcgtgc atgagcccca
    20761 gcgccagcgc gccgtcggtg ccgggcatca gtgcgacgtg ctgatggcac ctgtcggcgg
    20821 tttcagtgcg cctcgggtcg atgcagatca gcttggcgcc ctggcgcttg gcctcctggg
    20881 ccatcgtcca gaagtgcagg ttcgagccaa tcgagttgct gccccagatc aggatcaacc
    20941 ggctctcgac gaaatgctcg acatgcatgc cgaccgtgtc gccataactc gcagccatgg
    21001 cctcggtgcc ggcaccggcg cagatcgtgc ggtcgagcag agaagcgccg agcctgtgaa
    21061 agaagcgcgc ggccatgctt tcgccctgca ccaggcccat cgtgccggca tagctgtaag
    21121 gcaggatggc ctgcgggtcg cgcgaagcga tgccagacag gcgagcggcg atgtcgcgca
    21181 gcgcatcgtc ccagctcacc ctttcaaatc gcgccccgcc accggtcccg gacttcggtg
    21241 cgatgcgctt cagtggatgc agcacgcggt cggggtggta ggtgcgctcg gggtagcgcg
    21301 agaccttggt acacagcgcg ccatgggtcg cgggatggtc gggatcgccc tgcaccttga
    21361 cggcgatgcc gtccgtcacc gtcacctgca tcgcgcacgt gtccggacag tcgtgcggac
    21421 aggccgcgcg gatgatgcgg gtgttcatcg aaagatgatg tcacagcggg tcccgcagcc
    21481 tggcctcgtc tgcgacgggc atctcgtcgc ggatgaatcc cgcgggtcga tcgctacact
    21541 ggattcgttg ccgcggcctg ccgtcaacaa cgcccacctt ccgtgcctga atgctgccgg
    21601 ggctctcacc atgcgactga tcgaatccat cctctcggac gcggcggcca tgaccgcgct
    21661 gcgccgcgac ctgcatgcgc acccggagct ttgcttcgcc gagcagcgca cttccgacgt
    21721 catcgcgcgc cagctgagcg actggggcat tccgatacac cgcgggctgg gcaccaccgg
    21781 ggtggtggcc atcctgaaga acggaagctc ggaccgcgcg gtcgggctgc gcgcggacat
    21841 ggacgcgctg ccgatgaccg aacagaaccg cttcgagcac gccagccggc acgcagggaa
    21901 gatgcacgcc tgcggtcacg acggtcacac gacgatgctg ctggcagcag cgcatcacct
    21961 ggcacgtcat cgaagctacg acggcaccgt gtacctgatc ttccagccgg ccgaggaagg
    22021 tggcggtggt gctcgcgaga tgatcaagga cggattgctc gatcggttcc cgatggaagc
    22081 gatcttcggc gtgcacaact ggcccggaat gccggtcggc cagttcgcga tccgcaacgg
    22141 tccctgcttc gccagcagca acgagttccg catcacgatc cgtggaaagg gctcgcatgc
    22201 cgcgatgccc aacctcgggc tcgatccggt cccggtggcc tgccagatgg tgcaggcctt
    22261 ccagaccatc atcacgcgca acaagcggcc catcgacgcc ggcgtgatct cggtgacgat
    22321 gatccatgcg ggcgaagcca ccaatgtggt gcccgacacc tgcgaaatcc agggcacggt
    22381 gcgcaccttc acgctggagg tgctcgacct gatcgaacgc cgaatgctgg aagtggcgca
    22441 gcacaccagt gcagcgttcg gcgtgacctg cgagttcgag ttcactcgca actaccctcc
    22501 gacgatcaac cacccggccg agaccgcctt cgcccgcagc gtgatgaccg atctggtcgg
    22561 tgcagacaac gtgctcgagt tcgatcccac gatgggcgca gaagacttca gctacttcct
    22621 gcaggcgctg ccgggggcgt acttcgtgat gggcaatggc gatggcgatc acaggaaccc
    22681 ggggcacggg ctcgggccgt gcatgctgca caaccccagc tatgacttca acgacgatct
    22741 gattccgctg ggggcaaccc tgtgggtgcg gctggccgag cagtggcttg cccgcccgca
    22801 gcgctgacgc actggacgcc gccgcacacc agcgctggcg tctgcacctg gcacgtttcg
    22861 tgagcgggct tggggcatga ctgagctgaa cttcttttct gcgacgtacg ccgaagcccg
    22921 ggagcgcttc gtcgccgcgg caactcgatg cgacctggac attgaatcgc acccgcaccc
    22981 gctcatcggg ccccaaggcg aggcgctcgc gctcgatgtg gtgcgtgacg gcctgcgtcg
    23041 cgcaccacgg ctgctgatca tcagcagcgg ctgtcacggc atcgagggcc acgtgggctc
    23101 cgcagtgcag accgccttgc tgagcgaccc cgcctggcgg gcggcgacgc gcgaggccgg
    23161 cgtcgccgtg ctgtacctcc tcgcactgaa cccgcacggg ttcgcctggg gtcgcagggc
    23221 aacccaggaa aacgtcgacc tgaaccggaa tttcctcgac ttcgcacagc cgctgccgca
    23281 aaacgcagcc tacgacgagc tggccgccct gctggtgccc acccactggc caccgaatcc
    23341 ggcaggtgag gcggcactcg accgactgct ctctcaacgg ggctggcccg agatgcagcg
    23401 cgccatttcc tgcgggcaat acggacaccc agagggtctg ttctacggcg gccgcaatcc
    23461 aacgtggagc catgtcacgc tgcggcaggt gcttcgcgaa cagggacgtt cctgcgccca
    23521 gctgggctgg atcgatgtgc attcggggct cgggccctgg ggagtcggcg aacgcatctt
    23581 cgaaggtcgc gccggggacg aggccgaggg actgcgccgc gctcgggcct ggtggggcga
    23641 ccaggtcact tgcatcgaag acggcgaatc ggtgtcgtcc tcggtgaccg gacagatgat
    23701 gcaggcgctt tactccgaat gcccgcaggc gccttgcacc gggatcacgc tggagttcgg
    23761 aaccgtgccg ctgatggagg tcgtgtctgc actgagagcc gagcagtggc tcgagaacca
    23821 cccggaatcc gaggggtcga tcggtcgtga aatcaggaag cgctttcgcg ccgcgttcta
    23881 ccccgacagc gattcatggc ggcagcaggt gctggcgcag gccctcgaag cggcccggca
    23941 ggcagtggct ggactgcaga cggcctgagc gcccggctcg tgcgagcggc ttcttcagtc
    24001 aatcaggcca agggctcgca aatcgtctgc catggcctgc ggcccggtga acagcctcgc
    24061 ctgccaccca tggcgaagtg ccgcttcgat gttctccggg tgatcgtcga tgaacagcgt
    24121 gtccgccggg tcaccgccaa agcgttgctg cgccagcgtg aatatcgctg gatcgggctt
    24181 ggtgatcttc acatgcgacg agtagatgcc gctgtcgaaa agcttgagaa acgcgtgccg
    24241 atggctcaga tggtgggcca gaggcgccgg catgttggaa aggtagtgca gccggtgtcc
    24301 gcgttcgcgc agggcaccca gcagccgcac gctgtcgtca atcggctgca gttcgtcggg
    24361 aaccgcgtcg accaccgcac gcacctccgc ctcggaaagc ccggtgcggc gcgaaatgcg
    24421 cggcaccatc tctgacacgg tggtcactcc gcgatcgaat tcaccccact cgccgcggta
    24481 ggaaagaaag aactgctcct tccaatgctg agcactcagt tcggccgtga tgcgaagtgg
    24541 caacgtgcgc gcgagcagcg ccgccggatc ccagcggaac agcacgccgc cgaaatccca
    24601 gacgactctc atccacgcag ccgcgtgatc aggccagcgg tcgatccgtc gagagccgtg
    24661 ccatcgagat cacccgacag cagtcgcatc tgcacgtcct tgcacagcgc cttgccgagc
    24721 tcgacgcccc attgatcgaa gctgttgatg ccccagatgg caccgctggt gaatatgcgg
    24781 tgttcgtaca gcgcgaccag cgcgcccagg ctgtgcggcg tcaggcgatc gagcagcaag
    24841 gtcgtgctcg gccggtttcc aggaaagctg cggtggcgcg cgagggttgc cgcatcgagc
    24901 gcgcccgcgt cgggatggct tccttcagcc aggacctgct cggcagtctt gccacgcatc
    24961 agcgcctgcg attgcgcgag cccgttcgcc agcagcatcg tctgctgcat ctcgagccgc
    25021 tccagcagat cggcattcag cccgctgctg ccgaaggtcg gcctgcgcac gagaatgaat
    25081 tcgacaggga tggcttcggt gccctgatgc agcatctgga aatacgcatg ctgcccgttg
    25141 gtgcccggct cgccccagac cacgggactg gttgcgtaag gcagaagctc gccagcgcga
    25201 tccacgcgct tgccattgct ctccatctcg agttgctgca ggtaggcagg caggcgtctt
    25261 agcccctggt gatacggcgc gatgctgcgg ctggcggccc catggaagtt gcggtaccag
    25321 acatcaagca ggccgagcag agccggcacg ttggacgcga gcggcgcctc gacgaagtgt
    25381 cgatccatcg cgtgcgcgcc ggccagcaac tcccggaaat gatcggcccc gatcgcgatc
    25441 gcaatgggca agccgatcgc cgaccagaga gagaagcgac cgccgaccca atcccagaaa
    25501 ccgaaggtgg tcgtgatgcc gaattcggcg gccgccttga cgttggtggt cgttgcgacg
    25561 aagtggcggg caatgtcctt gccgccttcg gcctcgaacc aggcgcgcgc caggtgcgcg
    25621 ttggccatcg tctcctgcgt cgtgaaggtc ttgctcgcga cgatgaacag cgtttcctcg
    25681 ggcttcagtc tccgcaacac cggcgtgatg tcgtggccat cgacgttcga gacgaaatgg
    25741 aactgaaggc ccgggtgcac gtaggcatcg agcgccggca cgaccatctg cggcccgagg
    25801 tcgctgcccc cgatgccgat gttgacgacg tggcggattc tgctggtcgc gctgtcgcgc
    25861 accgtctcgg cccaggccag catcgcatcg agcacgccgt ggacctgagc gctgaaaggc
    25921 ccccgaccgc gcggcgaacg cagcgcggtg tggagaaccg cgcggttctc ggtcgtgttg
    25981 atcgccgcac cggcgaacat ggcgtcacgc tgcgcctcca ggccacattc gcgcgccagg
    26041 tcgacaagaa accggagtgt ggcgaggtcg aggtggttct tcgacaggtc ggcaaagacc
    26101 tccggcgcct cgaagctcag cgaagcaaac cggccggcat cgcgggcgaa ggcctcacgc
    26161 aggtcgaaat cgcgcccgtg cgcctcgaaa tgtcccttca gggcgcccca ggcaacggtt
    26221 ctgtcgcagc gcatgaaaac tgcccgaccg gtcacagcgc gcgaatcagc gcgtcgagct
    26281 tgccggcgtc gaccgcgaac gcgcggatgc cttcggcgag cttctccgtg gccatcgcgt
    26341 cctcgttgag cgcatagcgg aaactggcct cggtgtgggt cactctctcg atcgctgcgt
    26401 cgcgcgctgc agccggatcg agcgcccggg cgaccggtgc ctcgctggcc tgaagcgccg
    26461 cgaggagttc cgggctgatc gtcagcagat cgcagccggc cagcgccgtg atctgcccca
    26521 cgttgcgaaa actcgcgccc atcacttcgg tggcgatgcc gaacttcttg tagaagcggt
    26581 agatctgggc caccgatctc acgcccggat cgttgactcc ggcgtttgcc gcttcatccc
    26641 atcccgcgcc ggcagccttc ttgtaccagt cgtagatgcg cccgacaaac ggcgagatca
    26701 actgcacacc ggcctcgcca caggctacgg cctgcgcgaa cgaaaacagc agcgtcaggt
    26761 tgcagtgaat gccctcgcgc tccagcaccc gggcagcctg gatgccttcc caggtcgagg
    26821 caatcttgat cagcacccgc tcgcggccga tccctgcctg ctcgtacagc gcgatcaggc
    26881 gccgcgcacg cgccagcgtg gccgggccgt cgaagctcaa ccgggcgtcg acctcggtgg
    26941 acacgcgacc gggcacgacc ttcaggatct cgagcccgaa acgcaccagg atgcggtcaa
    27001 cgatctcatc gagcgccacg ccccggtgcg aggcagcggt gtcggtgagc agcggcgcgt
    27061 aatcgggctg ctgcaccgcc ttcaggatca gcgaggggtt ggtggtcgcg tcgcggggcg
    27121 caaacgcggc gagttgcctg aagttgccgg tgtcggcaac cacggtggtg aaacgcttga
    27181 gttggtcgag ttggttcatg aaagtgattc gatgcgcgta cgccggatta gaacccgatc
    27241 gggtgacatc cgagcacagg ttgccgatcg catccggcat caatcctgcg tggtgaaatg
    27301 cgtccagcga cacgggcagg agggccccca cgatgtcttt cgacctggtt ttcttcggcg
    27361 gcaccggcga cctgacgtgg cgcaagctga tgccggcgct gttccaggcg tttcggcacg
    27421 gcaagctgcc tccgggcggg cgcatcctgt ccgtcgcccg tgacgagcaa agtgacgagc
    27481 agtaccgggg ctggctgaag gagcgcttcc aggacgtcga gggcggcaag cgtccgaccg
    27541 acgaggagtt ctcgcgcttc gctgaattgc tgcactaccg tcgcatggac ctgtcgcagc
    27601 cggagcacta tgccggcctg aagcaatggc tcgacgaccg gggggccgac accgcggtgc
    27661 tgtatctcgc caccagcccg cacctgttca cgcagatctg ccagcaactg ggcgccgtcg
    27721 gactcaacgg accgaaggtt cgcgtcgtgc tcgaaaagcc gctcggccac gacctggcga
    27781 gcgcgcagga aatcaaccac gtcgtgcgct cggtgttcgc cgaaacccag gccttccgca
    27841 tcgaccacta cctcggcaag ccgtcggtgc agaacctgat ggcgctgcgc ttcggcaacg
    27901 cgctgttcga accgctgtgg cgacgagaaa gcatcgccaa catccagatc acgctggcag
    27961 agagcctggg cgtcggcacg cggggcgact tctacgaccg aacgggcacc ttgcgtgaca
    28021 tggtgcagaa ccacgcgctg cagctgctca gcatggtggc catggagcca ccgtcgcgct
    28081 acgacgccga cgcgatccgg gacgagaaat tgaaagtcct gcgctcgctg aaaccgttca
    28141 ccgacgacag cgttgcgcgt gacgtggtgc gcggccagta ccgcgctggc aacaccgccg
    28201 gtcaggcagt ggtcgggtac ctcgacgaag tcaaggtgcc atcaggcagc cgctgcgaaa
    28261 ccttcgtcgc actgcgcacc gaagtgcaga actggcgctg ggccggcgtg ccgttctatc
    28321 tgcgcaccgg caagaggctc gcagctcgcg atgcgcagat cgtcgtcaat ttccgtccga
    28381 cgccacacag catatttccg gggctgaacc gccccaacaa gctggtgatc aagctgcagc
    28441 ccgaggacgg cctcgaactg cacctgctcg cggccaaggg aaccggccag agcgaggccc
    28501 tgtcggcggt gtcgctcgac ctcgatttcg acaaggcctt tccggaaacc cgggtcggag
    28561 cctacgagcg cctgctgctc gaagccatcg ccggacggct caacctgttc gtgcgtagcg
    28621 acgagcagga gcaggcgtgg cgctgggtcg agccggtact gcacgcgtgg aaacgagaca
    28681 acgacgccgg gggcggtcca cgtccctacg cggcgggcac ctggggaccg gcagcagcga
    28741 gcgcgctggt ggctcgagac ggtttcgcct ggtcggaaga gcagtgagtg cgcagcccgg
    28801 ccgggcaccg ggcgacgact gcagcaacgg attcaggcca gccgggcagc gcccttgacc
    28861 ggcgcctcga tgcccagcaa tacggcaacg ccgtaaagac ccgccagctg gttcaatcgc
    28921 ggcggcgcgt tccgcaggac gaactccctg cgggacgatt gcgccagccg gcggcattcg
    28981 agcagcaccg ccagcactga tgagtcgaag tcgtgcaggc tagaggcgtc gacgatcaac
    29041 ggtgcggaag ggttctcggt cacgacacgc gtgagcctct tcagcagatc cttcgcgtcg
    29101 ccgagcagga ccgacggcgg aagcgccagc tcggcggccg aggatgtcac ttgcgtgccg
    29161 cagccgtctt gttgcgctcg gtcagcttgg caatcaagcc gtcgatgccg ttcgcgccaa
    29221 tttcctgcgc aaagctgttt cgataattct cgaccagcca gacaccgagc acattgacgt
    29281 cgtagatctt ccagctggag ttggacttct cgacgcggta atcgagttgc acggggtcac
    29341 ccttgccgcg aacctcggta cgaacgatga cctcgttacc ttcgggggca ttgcgcgaag
    29401 gcttgatctg cacggtctgg tccttgacct gcgaaagcgc gccggcgtag gtgcgcatca
    29461 gcagggtctt gaattcggac tccaggctgc tctgctgctc gggcgtggcc tgacgccagt
    29521 agcggcccac cgcggatgac gtcatgcgct ggaagttgac gtgcggaatg accttttcct
    29581 caaccagcgc ctggatcttc ttcatgtcac cggactggat cgacttgtcg gccttgacgg
    29641 tgtcgagcac ctcgttcgac agcttgttga tcaagtcgtc aggcgacgtg gcgcctgcct
    29701 gggcaaccac agccggggcc tccgcgtgca gggcgggcga aaccagccct gcaagcaagc
    29761 ccgcgcacat cgcggcgacc aatttcttct gcatcatctg gctttccttc tcgtgtggac
    29821 cccaagggca cctgcgttgg agcggaagat gctccgaaag ttcgttggtc tcggcccgct
    29881 ctcgcctcta cccctgccca ctcgggatca ggcgacggag acgctcgtta ttttgtctgt
    29941 gaagcagggg cctgtggcgg attgccgtcg tacaccagac tgttgcggcg ctgcaggtaa
    30001 ccgtcacgca cgaatgtgta cttgtccaga accgcgtcgt cgaggatgcc cgtggcattc
    30061 agcaggtttg cgcgcgtatt caccacgttc aagacagcca aaccgattcc ggcttccgtg
    30121 tcttcgatca ggaacccagg cgtgacatac atgtcgagcg gcaacccgaa cgaatcacgc
    30181 acggacgatg gtcccagcag cggccaaacg atgtaaggcc cggcaccgaa tccccagaca
    30241 cccagcgtct gcccgaaatc ctcgggaaag cgctcgatgc ggaactcggt cgcccagtcg
    30301 agcaggccac cgaaaccgag gacggtgttg gtgccaacgc gcatcgcgtt gctgatgcca
    30361 ttgccgacct tgccctgcag cacgctgttg accgccgacc aggcatcctt gaaattgccg
    30421 aagaaattgg tgacgccggt gcgaaccatg ctcggcacca cattgacgta gcccttggcg
    30481 accggcttga tcgcgttctc atcgaccgct tcgttgaacg cgaaaacgct gcgattccag
    30541 gattcgatcg ggtcacccgc gctgggagcg ctgcctgccg gcgcagtggc acagccctgc
    30601 agtgccacgg ccagcgcgat gcaggccagc ggcttcaatc cggatcggaa cgtgggggtc
    30661 attgtttttc tccttctgtg gcccgagcag ccggagcgcc ttccgacgcc ttgttgaaca
    30721 gaaactgact gatcagattc tccaacacca cggccgactg ggtctgcgtg atgacatccc
    30781 ctgctgccag attcttctcg ttgccgccgg gttcgatccc gacatattgc tcgccgagca
    30841 gccccgacgt caggatcttg gccgagctgt ccttgggaaa ctggaacttc tgctgcaact
    30901 gaagcgccac gctggcctgg aatgtcttgt cgtcgaaact gatcgattcg acacgcccca
    30961 cgacgacgcc agcgctcttg acggcagccc tcggcttcag tccaccgatg ttgtcgaacc
    31021 gggccaccac gccgtaggtg ctgtcgaagt tcagactgag caggttgccg gccttcagcg
    31081 ccagaaagag caaagccgcg gcgccgatca tcacgaacag ccccacccac acttcatgac
    31141 gggatttttg caacgtcatt ccccttgtcg accgctgcgg gtcattagat cgaaaacatc
    31201 atggcggtca gcacgaagtc gagcgccagc accgcaagcg acgacgtgac caccgtgcgc
    31261 gtcgtggccc gggatacccc ctcgggcgtg ggcttgcagt catagccctc ccacagcgcg
    31321 acgaacgtga ccgtcacccc gaacaccacg ctcttgatgg cgccgttgcc gacatcggac
    31381 caccagctga caccgccctg catctgcgac cagaacgcgc cttcgtcgat gccgatcagc
    31441 aacacggcga ccgcccagcc acccagcacg cccacggcgc tgaacacggc cgccagcagg
    31501 ggcatcgata tcaggccggc cagaaagcgc ggagcgagca ctcgacgcac cggatcgacc
    31561 gccatcatct ccatcgcggc cagttgttcg ccggccttca tcaggccgat ttcagccgtc
    31621 agcgaagtgc ctgcgcgtcc cgcgaacagc agcgcggtga ccacgggacc gagttcgcgc
    31681 accagcgaca acgcaaccag caggccgaca gcttcggcag aaccgtagcg ctgcagcgtg
    31741 tagtaaccct gcaaggcgag gacgaaaccg acaaacaggc ccgacacacc gatgatggac
    31801 agcgagcggt tgccgacgaa gaaggtctgc tcgatcacca gggtgaaccg gcgcaatgcc
    31861 agtggcgtca gcgccaccag ccgaacgaac aggaccgccg cggcaccgat gccggccatc
    31921 tgagccagca ccgtgcgtcc gacccaggcc gggttgagcc agttcatgcc cggcctccaa
    31981 cgccgaaatc ctcggtcacc gaggcagccg ggtaatggaa ctccacgggg ccgtccggcg
    32041 aggccgtgaa gaactgatgc acgagcgggt cggtgctggc ccggatctcg tccggcgtgc
    32101 cctgcgcggc gatgaccccg ttggccacaa aaacgatgtg gtcggcgatg gcgaaggttt
    32161 cctcgacatc gtgagacacg accacgctgg tgacgccgag cgcctggctc aggtcacgta
    32221 tcagccgcga cgcgctgccg agcgaaatcg ggtccagccc ggcaaacggt tcgtcgtaca
    32281 tgatcagatc agggtcgagc gcaatggccc gggccagcgc cacacgccgt gccatgccgc
    32341 ccgaaacttc ggaaggcatc aggttgcgcg caccgcgaag acccaccgca tcgagcttca
    32401 tcagcaccac gtcgcggatc atcgagggac tcaggttcgt gtgctcgcgc agtggaaatg
    32461 ccacgttgtc gaacaccgac aaatcagtga acagcgcgcc gaactggaac agcatgccca
    32521 tgcggcgacg caacgcatag agcccgcgcc gatcgagctt ggcgagatcg atcccgtcga
    32581 agaacacctg accgctcatc ggcgccaact ggcgtccgat caggcgcaac aacgtggtct
    32641 tgcctccgcc agaagtgccc atcacaccga tcaccttgcc acgcggcaga tcgatgtcga
    32701 tgttgctgag gatgacccgg tcgccatacc cgcaggtcac tccgcgcatg gaaacgagga
    32761 ccggtgcggc ggcaggttga gggtctgaga gagaagtcat ggggacggga tgtcgcgggg
    32821 acggttgccc cccgctgcgc tgcgcatgat aggtgagatg tcaacgaccc gacgcccctg
    32881 tgggtgggtg acaacccgtt gccagcgggc gcctggtcgc cgaaatcagc gcggcaggtc
    32941 gctgcggccc atcaggtgcg cgtcgatcgc gcgcgcagcc tggcggccct cgcgtatggc
    33001 ccacaccacc agcgactggc cgcgacgcac gtcaccggca gcaaagacct tcggcaagtt
    33061 ggtggcgtaa ccggtgacat cttcggtgcc ggcacgggca ttgccgcggg catccttgtc
    33121 gatgccgaat gcatcgagca cgctggctac cggactcacg aagcccatgg ccagcaggac
    33181 cagatcggcc ggcaggatct gctcgctgcc gggtacctcg accatcttgc cgccctgcca
    33241 ctcgacacgc acggccttca gcgccttgac cttgcccttg tccttgccct cgccgccgat
    33301 gaattccttg gtcgcgatgg cgaactcgcg atcgcagcct tcctcgtggc tcgacgaagt
    33361 gcgcaacttg gtcggccagt aaggccagac caggggcttg ttctcgtgct cgggcggcat
    33421 cggcagcagt tcgaactgcg tgacgctggc cgcgccatga cggttgctgg tacccacgca
    33481 gtcgcttccg gtgtccccgc cgccgatcac cacgacgtgc ttcccatcgg cgcgcagctg
    33541 gcccttcagc ttgtcaccgg ccacgacctt gttctgctgc ggcaggaact ccatcgcgaa
    33601 atgaacgcct tccaggtcgc gccccggcac cggcaggtcg cgcgactgct cggcaccgcc
    33661 ggcgagcagc accacgtcga actgggcctg gagctgctcg ggcgaaatca cttccttggc
    33721 ccagttggtg accttgctgc cctcgggcag gcgccccacc atgacaccgg tgcggaaggt
    33781 cacgccctca gcctgcatct gctcgactcg ccggtcgatg tgcagcttct ccatcttgaa
    33841 atcggggatg ccataacgca gcaggccacc gacacggtca ttcttctcga acaccgtcac
    33901 atcgtgcccg acgcgcgcga gctgctgagc cgcggccagg cccgccgggc cggaacccac
    33961 gacggcgacg gacttgccgc tgcggtgctt cggcggacgc ggcgtgaccc atccctcggc
    34021 ccaggcgcga tcgatgatcg cgtgctcgat gctcttgatg ccgacggcat cgtcgttgac
    34081 gttcagcgtg caggccgctt cgcatggcgc cgggcagatg cgtccggtga actcggggaa
    34141 gttgttggtc gagtgcagca cctcgatcgc gttcttccag tctccgcgaa acaccagctc
    34201 gttgaagtcc ggaatgatgt tgttgaccgg gcaaccgttg ttgcagaacg gggtaccgca
    34261 atccatgcag cgagcgctct ggatgcgtgc ctctccctcc ttgagcccga tcgcgacttc
    34321 cttgtagttc ttgagccgca gctgaaccgg ttgataggcc tcttcgaggc gctcgtactc
    34381 catgaagccg gtgatttttc ccatgatgcg attgattcct gctggcttgc tgtttgattc
    34441 gatgcggcgt gcgggtcggt cggcgcgtca agacatcgcc gatctgtcgt acgtcacttg
    34501 gacgaaggag acgccttggt cttcttgtcc gaagccttgg ccttgctcgt caccaccgtg
    34561 gcagacgcat cggcttcggt gttcacggac cgagcccggg cttccttcgc ggcgagttcg
    34621 gacagcgcgc gcttgtactc gttcgggaac accttgacga acttcgcgcg ggaggcctcc
    34681 cactgatcga gcaggtgccg cgcctgcagc gacccggtcc acttgtggtg gtcctcgatc
    34741 agcttcctga ggatcgcgtc gtcggtctgt cccttgtgcc acagcgcagg atcggtggcg
    34801 ccctgctcgg cgggcgacag cacgcgctcc aggctgacca tcgaggtgtt gcagcgcgac
    34861 gcgaacttcc cttccgggtc gtacacgtat gcaacgccgc cgctcatgcc agccgcgaag
    34921 ttgcggcccg tctcgccgag cacgaccacc gttccgccgg tcatgtactc gcagccatgg
    34981 tcaccggtgc cctcgaccac cgcagtggct ccggacaggc gcaccgcgaa ccgctcgccg
    35041 gccacgccac ggaagaaagc ctctccgctg gtggcaccgt aaagcacggt gttgccgacg
    35101 atgatgttct tcatcgcgtc gccgcggaac tcgatgctcg ggcggatggc gatgcgcccg
    35161 ccgctcatgc cttttccggt gtagtcgttg gcatctccaa tcaggtagaa ggtgatgccc
    35221 ttcgcgagga acgcaccgaa gctctggcca ccggtgcctt ccatctggat gaagatcgtc
    35281 tggtctggca gaccctcggg atggcgacga accagttcac cggacagcat cgcgccgacg
    35341 gtgcggttga cgttgcgcac ctcgtgcatg aactgcacct tctccccgcg ctcgagcgcc
    35401 ggcttgcact tctcgatcag cgccacgtcg agcgcacggg ccagtccatg atcctggact
    35461 tcgacctggc ggcgaggcac ttcggccggt gccgccggca gatggaagat gcgcgcgaaa
    35521 tcgagtccct tggccttcca gtgctcgatg cccctcttgg tgtcgagcag gtcggcgcgg
    35581 ccgatcaggt cgtcgaactt gcggatgccc agctgcgcca tgatgcggcg cgcttcctcg
    35641 gcaacgaaga agaagtagtt caccacgtgc tcgggcttgc cctggaactt ggcacgcagc
    35701 gccggatcct gcgtggccac gccgaccggg cacgtgttga ggtggcactt gcgcatcatg
    35761 atgcagccct cgacgaccag cggcgccgtc gcgaagccga actcgtcagc ccccagcaac
    35821 gcaccgatga ccacgtcgcg gccggtcttc atctggccgt cggcctgcac gcggatgcgg
    35881 ctgcgcaggc ggttcagcac cagcgtctgc tgcgtttcgg ccaagcccag ctcccacggc
    35941 gtgccagcgt gcttgatcga actccagggc gatgcgccgg tgccaccatc gtggccggcg
    36001 atcaccacgt ggtcggcctt cgctttcgcc acaccagccg cgatcgtgcc gaccccaacc
    36061 tcgctgacca gcttgacgct gatgtcggcg cgctggttgg cgttcttcag atcgtggatc
    36121 agctgcgcca gatcctcgat cgaatagatg tcgtggtgcg gcggcggcga gatcaggccg
    36181 acgccaggca cggagtagcg caggaagccg atgtactcgc tgaccttgcc gccgggcagc
    36241 tgtccgccct cacccggctt ggcaccttgg gccatcttga tctggatctg gtcggcgctg
    36301 gacaggtatt cggtggtcac cccgaagcgg ccggacgcca cctgcttgat cttgctgcgc
    36361 agcgaatcac cggcctgcag ttcgaagtcg gccacgatga ccttgtcgcc gagcacgtcg
    36421 ctgatccggg tgccggcagc gatcgggatg cctttcagct cgttgcggta gcgcgccggg
    36481 tcctcgccgc cctcaccggt gttgctcttg ccaccgatgc ggttcatcgc cacggcgagc
    36541 gtgctgtgcg cctcggtcga gatcgatcca agcgacattg ctccggtggc aaatcgcttg
    36601 acgatctcgg ccgccggctc cacttcgtcg agtggcaccg ccttgctcgg gtcaagcttg
    36661 aactcgaaca acccgcgcag cgtcatgtgg cgacgcgact ggtcgttgat cagctgcgcg
    36721 tattccttgt aggtctcgaa ctggttggca cgcgtgctgt gctggagctt ggcgatggca
    36781 tccggcgtcc acatgtgctc ttcgccgcgc acgcgccagg cgtattcacc cccggcatcg
    36841 agcatgccgg ccagaaccgg gtcttctccg aacgcggcgt tgtgcatgcg cagcgcctct
    36901 tcggcgacct ggaacacacc gatgccgccg acctgcgatg cggtgcctcg gaagtacttg
    36961 tcgaccatcg tcttctcgag gccgatcgcc tcgaacagct gcgccccgca atacgacatg
    37021 taggtggaca cgcccatctt ggacatgatc ttcgacaggc ccttgccgat cgccttgatg
    37081 tagttgtaga tcgccttctc ggtcgacagg tccgccggca ggtcgcgatg catgttcgcg
    37141 agcgtctcca tcgccaggta cgggtggacg gcctcggcgc cgtagccagc cagcatgccg
    37201 aag
//
LOCUS       DAOORV010000017        35980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1367_length_35980_cov_7.416841, whole genome shotgun sequence.
ACCESSION   DAOORV010000017 DAOORV010000000
VERSION     DAOORV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 35980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_35980_cov_7.416841"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1478
                     /gene="eat"
                     /locus_tag="PKV56_04090"
     CDS             105..1478
                     /gene="eat"
                     /locus_tag="PKV56_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HOX67022.1"
                     /translation="MSSSTTQTLGKTLGTFQLWGIAVGLVISGEYFGWSYGWASAGTL
                     GFLVTSAFIALMYTTFIFSFTELTTAIPHAGGPFEYSRRAFGPTGGYIAGYATLVEFV
                     FAPPAIALAIGAYVNVQFPALDPKWVAVGAYIVFMGLNIAGMKIAASFELIVTLIAIF
                     ELLVFMGVVAPAFSVANLVKGGWSGADTFSIGSISGIVAAIPFAIWFFLAIEGVAMAA
                     EEAKTPHRSIPIAYIGGILTLVALATGVMVFAGGSGDWTKLANINDPLPQSMKTVVGE
                     SSGWLHMLVWLGLFGLVASFHGIIIGYSRQIFALSRAGFLPSALGQIHPKLGTPHVAI
                     VAGGVIGIAAIFSDQLIQIGGMSLTANIVTMSVFGAIVMYILSMASLFKLRISEPRLE
                     RPFSAPVFPLFPAFALGAALICLLTMIYFNPIVAGLFVGLMIVGYLYFLTTAGRRGEA
                     MEAARAY"
     gene            1502..2935
                     /locus_tag="PKV56_04095"
     CDS             1502..2935
                     /locus_tag="PKV56_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOX67023.1"
                     /translation="MPLYSRAVGVRNFRFDSLKTLLARATPERSGDRLAGVAAATAEE
                     RVAAQMTLADVPLRQFLNEAVVPYEDDEVTRLIVDSHDATAFAAVAHLTVGGFREWLL
                     SDEVDSAALAALSGGITPEMAAAVSKIMRNQDLIMVARKVRVRSRFRNTIGLPGRLST
                     RLQPNHPTDDTAGIAASLLDGLLYGSGDAVIGINPATDNVPQVIRLLQMLDAVIERYG
                     IPTQSCILTHVTNTLQAIERGAPVDLVFQSIGGTQATNASFGIDLAMLAEARAAALSL
                     KRGALFEDETRSERGDNVMYFETGQGSALSANGHHGCDQQTIEARAYAVARHFEPLLV
                     NTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQNDMDVLLT
                     VLGVAGCSFIMGIPGSDDVMLNYQTTSFHDALYARRVLGLRPAPEFEAWLERMGVFSP
                     GGNARLGRHASGLPPAFEHALPTPALR"
     gene            2958..3839
                     /gene="eutC"
                     /locus_tag="PKV56_04100"
     CDS             2958..3839
                     /gene="eutC"
                     /locus_tag="PKV56_04100"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HOX67024.1"
                     /translation="MPSESDPVKPTNPIAAEIESPVVTNPWSVLKQFTPARIGLGRTG
                     VSLPTSAQLDFQLAHAQARDAVHRPLEVQALCSDVEALGLETLPLHSAATTRSMYLQR
                     PDLGRQLDVSSRQRLEALRARRQSQGGQKSDLVFAVVDGLSALAVQRHAAPLLAEVLA
                     LLDRSVAGQVPRWTVAPVAVVEQGRVAIGDEIGGLLDGRIVVVLIGERPGLSSPDSLG
                     LYLTWEPQPGRHDAERNCISNVRPEGLGYRDAAARLVYLLEEARRRQLTGVGLKDESD
                     SAADVVSAQPNFLLRGT"
     gene            complement(3904..5052)
                     /locus_tag="PKV56_04105"
     CDS             complement(3904..5052)
                     /locus_tag="PKV56_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67025.1"
                     /translation="MKTSLPDGHHIVHLAGVVTDAVFSLLGPMTEVQAELGMEQTVIL
                     VDAPAHRHLLPRFHPTIRLVLMPGETGPLRRINRSLRALLTVIEAQPTRSVHLHGFIP
                     SLVGTYAKRFHGLSQTLYFSPHGSRLLKPITAVSALTVFLLKPVVRLSDQRTIANSSF
                     DATALKRVTNDKIPIVESPVDAAFFCATRHEARRPLLITGSRSRNRADAAMFAQLAVL
                     LGEESLEVSCNWLGTADDDSLARLSAANVAVFDVTDASERASRLSAAWVYVAFGGELG
                     FPVFLTEAMAAGLPCVVWDTPYHRQVIEEGVTGHCCGTYPAFLACIARLIDSPEERQR
                     LGSAARVEAQRRFDGKKFRDAYLAAYHAAPPKAEAVERSGRTLGKSSI"
     gene            complement(5096..5773)
                     /locus_tag="PKV56_04110"
     CDS             complement(5096..5773)
                     /locus_tag="PKV56_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOX67026.1"
                     /translation="MITATASSSDMHALLRPTNRASLRDTAYVVGNRLAALLLLCVFA
                     PAMALIALLVAHRDGMPVFFGHYRVGLNGKLFRCLKFRTMYRDSAQMLADVLRDDPVA
                     RSEWALHNKLSKDPRITPVGNFLRRTSLDELPQLFNVLRGEMTLVGPRPITVAELCRY
                     GQVRWDYLSVLPGMTGLWQVSGRSDTTYEERVALDLRYVEQRTIWLDAAILLKTVKVV
                     LARDGSR"
     gene            6125..7396
                     /locus_tag="PKV56_04115"
     CDS             6125..7396
                     /locus_tag="PKV56_04115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX67027.1"
                     /translation="MKILLVATRSVEGPRTGRKVVLATIVRSCKALGHQVDIAVIGPS
                     EPSTGGISIQAPGFLRVGHNFVRHFLLGRKSLNECLYWSRQTRRRIADLVVSGAYDMV
                     VADMLRSAQFCSGLKVPWILDLDDLLSDRYAGLAAAEDGRMLLGYFSHRLPVSLRRSA
                     AWIAHRLLKFESRRIAAQERRFGLLADAVTLVSSIEAAKLARQLGRAVHGTAMGIDVP
                     DLAPIDASRRHARAVAFMGGLDYQPNLAAVTHYLRCIAPELERLGCSVALHVLGHAPH
                     ELRPPDPVGLATYLGYVNDLESTLARYTIFVAPILADGGIKTKVLEAMANGLAVIATE
                     QAVAGLGITPGKECIVTRTPGEFARAVQALIDDPDGCRRLGAAAQRFVGLNFSRSVLE
                     IKWQQLLEDAASHFQLMAASSCNAGTSKPTR"
     gene            complement(7342..9282)
                     /locus_tag="PKV56_04120"
     CDS             complement(7342..9282)
                     /locus_tag="PKV56_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HOX67028.1"
                     /translation="MSNALYTQLAWLPAAPEDFSASCRHLLNDPSDLGRRLQHLANHA
                     LDENQLNRLAKVIDKARDAGQSLAPLTAFRIGIISNATSHFIVPALIASAARHGIALE
                     CIEADYDQVMQAALSADSPIHQAKCDAVLVAVDHRGLPLAFTPGDPQGAQASVQAALG
                     HLEMIRRGLHSNSKAICIMQTLPRPVESTFGSFDLVLPGTPRQLIDEINRGIAMSVAG
                     TEDLLLDVAHIAETVGLADWHDPTLWNMAKLPFASDFLPLYADHVCRLIASVRGKSRK
                     CLILDLDNTLWGGVIGDDGVEGIVIGQGDATGEAHLQVQRTALALRDRGVVLAVSSKN
                     TDEIARQPFRKHPEMLLREEHFAVFQANWNDKATNIKAIAEELSLGLDAMVFLDDNPV
                     ERGLVRRMLPQVAVPELPENAALYARTLLAAGYFEAIAFSGEDRQRANFYQDNARRVT
                     LQKQAGDIDAYLASLNMQMTLQPFDDTGRARIAQLINKSNQFNLTTRRYTEAEVAAAQ
                     IDPDAFTLQVRLTDAFGDNGMISVLICRRQGTDWHIDTWLMSCRVLGRKVEVAVLQEL
                     MSQAAERGVLRLIGNYLPTEKNKLVEDHYDKLGFTLLQKSEAGASTWELLVAGAPAAT
                     LPIAIQRVGFDVPALQDEAAMS"
     gene            complement(9279..9980)
                     /locus_tag="PKV56_04125"
     CDS             complement(9279..9980)
                     /locus_tag="PKV56_04125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX67029.1"
                     /translation="MDTETDVGRAADERWFLVSGGSGGIGSAVCEQLAARGYTPIVGY
                     HRNAAAAESLAQRTGGRALALDLGDDASILAAVATLEAAPRLFGVVLAGSPPLSPVPF
                     GKITTDEMRQQWQVNVMGPQRLLAELVRRCFRRHKQGSVVGVLTQAMGDGAAGAASGM
                     GAYVIAKHGMAGMLALLAADHPWLRVRSVKPGYTETRMLAAFDERFLAMQREKAAFQT
                     PEQVASLILEEAIGP"
     gene            complement(9970..10401)
                     /locus_tag="PKV56_04130"
     CDS             complement(9970..10401)
                     /locus_tag="PKV56_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOX67030.1"
                     /translation="MSSAPASRALALGELVEGLHACVEFSVTAAQMQQFAELSGDFNP
                     LHTDDAFARTKGFDGVVVYGALMVAKVSQLIGMQLPGRDSVWVGIALDFRKPLYVGQA
                     AQVEGVVTEASESTGMVALKVTVRANGKVLAKGTAEVVVGH"
     gene            complement(10398..10640)
                     /locus_tag="PKV56_04135"
     CDS             complement(10398..10640)
                     /locus_tag="PKV56_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX67031.1"
                     /translation="MSHDALYAKLTAIFHDVFDDEDIVVTPELTADDVDEWDSLSHIR
                     LVVAIEKKFGLKFSAAEVGRLKNVGEFASLIASKTA"
     gene            complement(10702..11790)
                     /locus_tag="PKV56_04140"
     CDS             complement(10702..11790)
                     /locus_tag="PKV56_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67032.1"
                     /translation="MKLVIKQILQLIAALMALYALLLTISLLLVPRFEGGLRLDASQA
                     SSSLFLTEPKYVFMARSRLNTPSDKVLLLGASNMLVGFRQAQVQALLPEVEVHNLSVG
                     GSNIGQVSQIVDLIREVQSPESRRHNTYVLGLWYGVFASDQARWNTPDRHAGDTDIDI
                     ERYRYGFYRRTQAGAVPVLPPMLLDTGVLLIHPYLVLDRTARDLTRSLRDFMASRPAS
                     MTDDQRNAVVIGDADKRRYLAFWRDYMGSTDSLEAQPFRLLQRTVEGILAEGGRVILV
                     DMPIPGWHTSASPLAADYRARLDSLLSGLKGRAGVAFLRMDDAHADEDFSDEVHPKPR
                     VTASWAQRLAAVIKTSESLPPSQSASAH"
     gene            complement(11790..13229)
                     /locus_tag="PKV56_04145"
     CDS             complement(11790..13229)
                     /locus_tag="PKV56_04145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOX67033.1"
                     /translation="MAFTSPIFIAFILVVVLLTQALPSPAGRSGVLLLASLVFIGSHV
                     DSIGQVGPLAAFLLLCYVAIEGVRRSRSAASLWMGAVAVIASFVVLKKFSFLDESLVL
                     PFPYLIIGLSYVLFRVLHLMIDAKQGELIERIAPLHFVNYTCNFLSFIAGPVQRYGDY
                     TLQQGRALALDEETVFQAFARLIKGFVKVGVVSAIFNYLFSSMSGRLLDPALAPAQPL
                     MCGLFVAAAVFYTVYLYANFSGYMDIVIGVGTLMGQELPENFNRPFTARSFLDFWSRW
                     HMTLSDWFRTYVFNPLMKTLASRFTSPQAGPYLGVLAFFVTFLIMGVWHGTTSVFVVY
                     GLLMGTGASINKLWQVFMTKRLGKKGYKALSERTAFIYLCRGLTVAYFALALTCLWVD
                     MRELSWLAGRLGFVGIAACYLGLALAAAAAFFAWDALMLPLGPIARVMTQRSGGVIVR
                     NLSLASQILLIVTVASFFHKAPEFVYRAF"
     gene            complement(13241..14020)
                     /locus_tag="PKV56_04150"
     CDS             complement(13241..14020)
                     /locus_tag="PKV56_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOX67034.1"
                     /translation="MNHLAKLFLVLLSVAASLGAIDLTAAAEPSSATASTEPASGPTP
                     FPADARNWPGKGVIRVFGWMTDNRKAFWLERERRQGSVVFVGDSLIGGWSSLSKDLPD
                     LRVTNRGIGGEVSRGLLFRFKEDVLDLHPRSIVLLSGTNDLSALQDIQQTRSNLVEML
                     DRAERSSPGVPIVLCTLPPRNHPKAPVDPRQLIELNTLIKSATQGRADVVVLDLYALL
                     ADPEGAPRAEYFAADKLHISPAGYRVFRDALVPLLNRFKQD"
     gene            14426..15649
                     /locus_tag="PKV56_04155"
     CDS             14426..15649
                     /locus_tag="PKV56_04155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67035.1"
                     /translation="MQQESSSPHFAAQASPARHPAILLLTYISPLQRWGSAKRSRFLI
                     DALKQHGVVDVLVLSFVDIADARPTLTVTEMDGVQVMELQLVQRGWRSRPRFDLMSGD
                     VSKQVARHIDLASYDLLVSRYVRPAMKLDLPEGVPLIVDFDDAVYDPPWRALRTLKSW
                     VGVLLRLFNDRVIVRTRLKGASWRRAHYLFCREAERQVFGWLPGAVLPNLPPAADRAG
                     PPDFSPPEQPALMFVGLLDYMPNLDAVDWFLDAIWPIVLGEVPQARFLIVGSGADELL
                     ERWRRRPGVETLGFVDSLADAYAQATACVVPMRSGAGTNIKALEPYLYGRMVIATPLV
                     VEGHHPLFNAGQDILVAPDAKGLARHCIDMLVAPRRASELAQRGHQRITSTLTEKNFQ
                     RILGDAVALLLCPRR"
     gene            complement(15763..17058)
                     /locus_tag="PKV56_04160"
     CDS             complement(15763..17058)
                     /locus_tag="PKV56_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOX67036.1"
                     /translation="MPLEHLKRLRRHLLAWRDISALKGQLADRVCEKPEPGESTLLRR
                     LLILPSDPWTLVGAKGDEAMMQAVTTRLRQYSADLQVGVITATPGAEAAAGQLAFASV
                     PAWDCTLAEAATQIKAFDPDAMVVLGADVLDGYYNPVTASRMLLLADVAARQGVRVSI
                     LGFSFNNRPSSLVKPVFEGLSKRVAINLRDRVSFDRFHSFCNAPCRLVADSAFMLEAD
                     FSGDTVQAVEHWSTSRRAAGDVIVGFNMHPMLFRHASADQINALVKSAVSSLRAVAQR
                     RPVSILLLSHDYRGHDGDDVCLAPIALALADELGPRLNYPTTRFSAAQLKAMASFTDG
                     VVTGRMHLAIATLGMGRPVAALTYQDKFQGLFAHFEYPERFLLTPAAAADASKLTAMV
                     EEFVDRLQELTQCVREHLPAVREASFVNLERVLPSNAPA"
     gene            complement(17096..18562)
                     /locus_tag="PKV56_04165"
     CDS             complement(17096..18562)
                     /locus_tag="PKV56_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOX67037.1"
                     /translation="MSNDLRAKVRSGVLWSAVQNWGVRLSSLLLFMVVARWLSPEQLG
                     LFAAATVVIAFLNLLSEQGLGEALVQREEITQEQINSVFWLNLGASLLIVGLLWFVAP
                     LIAALMKLPELVPILRVVSLSMPLTAASFGQLAMRRRRFEYRWLAKTVLASTMVSSMV
                     ALAMLFAGLGVWSLVAQSLTTAAVISALLWIRPSWHLTMKTDFAATRPLMAYGAKRVS
                     TSLLDFANTRYIELFLASTLGPASLGLYTVGVRVYQALMQVFSSTILEVAQNGFCRLS
                     ADRPGLIRAYFQSIAATAAIVMPVFILIAALAPSLTVTLFGARWVASAEVTRWVAMLG
                     AVQVLQVYNGTVYNAIGRPGVGLQFMIFKVMLTFSALFLARDAGLQVLLQAYVASQLA
                     TTPLSFWLVRRLIGVSLTQLFGTLWPYVLSSTFMALVALGVASLLEARGASAPLVLLG
                     ACAAAVSVYLLCLQFFAPAALQTGLRFLRPGRQPTAAA"
     gene            complement(18594..19637)
                     /locus_tag="PKV56_04170"
     CDS             complement(18594..19637)
                     /locus_tag="PKV56_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX67038.1"
                     /translation="MLKSLLLGSVNRAVKPFPSVQQTLAGWKTNVKRWAKLRYFASDV
                     IRAKRFMFWSERDPSYAKLSSELLFQFHKLEKGLCIPGTKRYFGRDPLVATCQLVERW
                     QAHGFSMQDPVFIGAIEALRAYRTRLEATPANAEDAPLIQRLLNSCLSHTTEAPQFST
                     PHAYRRTEDAADVFDRLCQDRRSVRSYSSTAVPLPLLQEAIATAQLSPSACNRQPCRV
                     HVYRDAAQIKQMLSLQNGNSGFGHLLSTLLVICADSRSFFDASERHEPHVDGGLFAMS
                     LILALQARGLASCCLNWCVAPEVDAEAHARGELPEHDQVIMYLAVGYASPDALVPRSA
                     RRDVGSIMTIHGA"
     gene            complement(19642..20844)
                     /locus_tag="PKV56_04175"
     CDS             complement(19642..20844)
                     /locus_tag="PKV56_04175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX67039.1"
                     /translation="MPKFVFLLQAIACKLWSGMGERTGVKGFHAFDGLRGYMAWWVVI
                     GHGLHLCGLQNIAPAILSSGDHAVNVFVSLSGFVISHLLLERREPYGRYLVRRAFRIF
                     PIYWFALACAMLLAQMYSSVYAGDWVFQHEMRAARAVATEAEFSKYLLLHVSLLHGLV
                     PDTWLPFSSTAFLAPAWSLSLEWQFYLVAPFLVAGLAKPGMSRLLTLLLLSASWVLFK
                     KVLPLQWQYPAVLPLSIHFFMLGILSRVFLQVLSSIGFWLIPVALLMSGLAPRSVSTE
                     VAVWGVFMAAACLQLRASQAKGAVRDLAWFSRFLEFVTSNSVARWLGEFSYSTYLIHI
                     TLFSLVGWIAAKLGGAWTQQIAAVSTSIAVILLIPLSYLLYRFIEKPFIRWGAALAKP
                     QGISLKAV"
     gene            complement(20844..22112)
                     /locus_tag="PKV56_04180"
     CDS             complement(20844..22112)
                     /locus_tag="PKV56_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67040.1"
                     /translation="MTTESLRVLFISAVPTDPPDAGNRVRIMTLTRSVIAAGHEVHFA
                     YFPMESCDLRAMQARFGPDRLHVLHSRLEPPRGGLLSRAARKLGRLMNIDQAFVWGLD
                     DWYDERANSELAALHSLHEYDAVFVEYVFASKALEAFPPDCLRVLDTHDCFGLRHRLY
                     LKAGMRPQWFSTSVADEDSGFRRADIVLAIQASESRDFKARVEGSKTRVLQVGHLIDI
                     ADAAPPSRREAAVFLGSANPINVAGARYFIDRVLPIVRRTKPEFQLLLAGAVSAEFED
                     QDGVVKLGFVPNLQDAFGAAMIAVNPVLVGTGVNIKLLDAMASAMPIVTTRSGARGVE
                     ELDGHALFVVEDEDPGAFANRILGLIDDADARRRLSAAARSAAEKWNAAQVSTLVGAL
                     VRKSTRCAALDAAVPSQARLALDPFNLGGT"
     gene            complement(22109..23203)
                     /locus_tag="PKV56_04185"
     CDS             complement(22109..23203)
                     /locus_tag="PKV56_04185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOX67041.1"
                     /translation="MNNARQDTDRTPQMAGDALRLTVIIPNYNYAEFVGQAIDSALAI
                     DWPDVQVIVVDDGSTDASRQVIARYGERIETVLQANAGQYMAYNAGYLLARGDVVIFL
                     DSDDLLERSVMQEIAAIWRPGLSKVQFRLRVVDGEARPLGSTFPQFHGAPQPDELRRW
                     AAATTTYPTPPGSGNAYSRAYLDQILPLDEACGRPGDSCCVAAAPFLGDVATIDAPLG
                     QYRVHGRNDGAASRLDASQFHLHVVRGRQRSAYVRQVARRVGIEVSDKALDLSLHYLP
                     YRLASLQLAQATHPIAGDSAWAVFTDVLRALQTPQGLSPKARWMLGLWAALVVLLPRK
                     AGNRLILWRFVPAERPQALRATLLKLGVIR"
     gene            complement(23200..24387)
                     /locus_tag="PKV56_04190"
     CDS             complement(23200..24387)
                     /locus_tag="PKV56_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67042.1"
                     /translation="MTALFAGMVMTDRFGIAIGSFPLGLTMPLAYVLLAVLLLEGRLA
                     LDALSLAMFSALATVGVVSFLLNASVDGGSGTSISSLSLMLAIYLPFVFTQARQTDTQ
                     GQWLWMMRTAGNVLLIVAIAGIVQFYAQFVIRTPWLFDISKLIPDPIRAQGIYNSAIP
                     VGSTFKANGLFCREPSNFSFWMALGLLIELTLFKRVKRMLCFGLALMLSYSGTGLLTL
                     AFGLLFPFRHRLLAWVGTALVLILGSNALAGDPLNLAFTLNRFGEFSARGSSAYARYV
                     APMFLVDANFDSSPWSIWLGHGPGTIQKNVAAFDSHDPTWAKLLFEYGIAGFVICLGF
                     VAYCLSRFAAPFQFRAVLFANWLVMGGYLLTPETVYLLYVALAMWPGPAPGDAASGRQ
                     DLA"
     gene            complement(24526..25383)
                     /locus_tag="PKV56_04195"
     CDS             complement(24526..25383)
                     /locus_tag="PKV56_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOX67043.1"
                     /translation="MTGHLPRWLQACAALMIAAGWPGVSPARSLDPAAPAPIASQGYR
                     LVKDWTFGRTVSNADELRDNFHTRFIYENGTLDTLKGEWQRYRDNDNHDFRNGRFALV
                     ARVSKELKNGQIESGMLRSRWSGQYGYIEGRMKVPTGRGLWPAFWINPQDQIWPPEID
                     IFEIVDNGRDTTKNSFHFLHLGEGKGRPSQATLLDKFGAYRPGFDYADGFHTFAVLWE
                     PGRVRHFVDEKLVVDRPFDWTHKGGADAGPAHVLVNLAVGGEWPGPPLRIEDFPARLE
                     IDFIRVWQK"
     gene            complement(25380..26552)
                     /locus_tag="PKV56_04200"
     CDS             complement(25380..26552)
                     /locus_tag="PKV56_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67044.1"
                     /translation="MTDRRALRVLLVDPSLFTAPYDAALTGGLIEAGVSPIWVTRPTR
                     KGDRQELPIERTDPFFYKRVDEATWLPARLKPLAKGAAHLAGLVALLRKIRANPPDVV
                     HFQWTVVPPLDALAMALIRRWCPLVLTVHDTVPFNGERLSLLQNLGFDLPMRLAHRVI
                     VHTDSGRQTLIARGIPPGKIATIPHGALQLQVPLPQRTERPDGRYSFVLFGEIKPYKG
                     VDVLIEAVHRLPDEIRRQTRVVIAGRPRMNLAPLEERIASLGLADTIELRPQRQSEEA
                     MAALFVEADSFVFPYRQIDASGVWFLTQSLGRWMIASRVGIFAADLVEGRQGVLVPPG
                     DSAALAAAMEQAVRQRLTGDAMPASDQWIGIGHATRNVYDALLPQHSGAALVEARS"
     gene            complement(26549..27595)
                     /locus_tag="PKV56_04205"
     CDS             complement(26549..27595)
                     /locus_tag="PKV56_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX67045.1"
                     /translation="MTIEAARSVLLVIPTLNEARALPGVLRSLRQDLPADARVTLVVA
                     DGGSTDGTVELVRQISESDPGLHLLINPRRIQSAAINLAAKTFGRGVDVLVRCDAHAA
                     YPAGFVLRLLETLDRTQADAVVVPMDSCGTTCFQRAVAWVSNSWAGTGGSAHRGGHRS
                     GFVDHGHHAAFRMSSFMRAGGYDESFTHNEDAELDCRQRRLGSQVYLDATIRLSYHPR
                     DTVRGLARQYFSYGKGRSRTVRRHPGSMRARQLALPANLVISTLAISLAPVYPLTLAW
                     PLAYVAALGFTSISIAWQQRSMCGLLSGFAAGTMHVCWAAGFFSGLAFVRESAWQPEH
                     PAAHDMSPSGRVTP"
     gene            complement(27585..28502)
                     /locus_tag="PKV56_04210"
     CDS             complement(27585..28502)
                     /locus_tag="PKV56_04210"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOX67046.1"
                     /translation="MSVAHPIGLKARTAVPPQEPAAAADERVLDRSIGDFIRQVRGLS
                     EAQVEQILGHQRLHGIRFGEAAVALQLATRDDVLWALSQQFHYPYAPGDGASFNSELI
                     AAVDPFHRQTESFRDIRSQLMLGALAPEQPRRALAVMSPNIGDGKSFFAANLAVVFSQ
                     LGARTLLIDADMRTPRQHALFGVPNDYGLSSILSGRSDTKAIHSLQDLPSLFVLPVGT
                     LPPNPLELLQRPAFGLLVNELLGKFDHVIVDTPSSTHGADCRVLAAKCGVAMIIGRRG
                     KSRMKALQGLVSEVTRGPAKFAGVVINDH"
     gene            complement(28509..29936)
                     /gene="epsF"
                     /locus_tag="PKV56_04215"
     CDS             complement(28509..29936)
                     /gene="epsF"
                     /locus_tag="PKV56_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HOX67047.1"
                     /translation="MNLSQLLLILRARWRSALFVFAMTVAIVAAFTATRSKTFVATAA
                     VVLDVKSPDPIAGIVLPGMNVSSYMATQVDVLQSERVMLRAVKALRIDEKAGLRAAWL
                     EQTEGRGDFNAWLAEGLGRKLEARPGKDSNVIQVSCTDRDPKFAANLANAVVAAYIET
                     SLELRTEPAKQFNALFDESTKELRESLEVAQAKLSQFQQKKGIVATDERLDIENARLA
                     ELSTQLVELQAVANDSSSRQNQAARMADQMQEVLNNPMVVSLTSDLSRQEARLTEISE
                     RYGAQHPQVLELRASIRQMRDRLALEKSRITGSLTANNSVNQSRLENLRNALDAQRNK
                     VLQLKGIRDEAAVLQRDVENAQRVYDAGFGKLSQSTLESQATQTNVSVVKIATEPPFP
                     SSPRVMLNLTVGLLLGAILGITTAALREVRDWRLRTESDVTDAMKLHLLGVIPDRRSG
                     RLPREARSLRSVAERVLGRAMLIAH"
     gene            30265..30828
                     /gene="greB"
                     /locus_tag="PKV56_04220"
     CDS             30265..30828
                     /gene="greB"
                     /locus_tag="PKV56_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOX67048.1"
                     /translation="MSKAFTRESDDSEDDEESGAPAMPIGVKNYVTPAGYARLRAELL
                     NLLDVERPKVVDIVSWAAKNGDRSENGDYLYGKKRLREIDRRIRFLTKRLDIAEVVDP
                     SVHHGADQVFFGATVRYINARGEARTVTIKGVDEADSSAAEISWIAPMARALLGARVG
                     DEVKVQTPAGADLVEVLSVQYPGACPG"
     gene            complement(30845..33124)
                     /locus_tag="PKV56_04225"
     CDS             complement(30845..33124)
                     /locus_tag="PKV56_04225"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOX67049.1"
                     /translation="MGIPTIETVKEALTFRSSWRRPPVASVASDAAAASFDALTIKLD
                     YLDPADVRRVRDAYRFADEAHLGQYRASGEPYITHPIAVAGLCADWKLDAQAIMAALM
                     HDAMEDCGITKAELIERFGAPTAELVDGLTKLDKLQFSTREESQAESFRKMLLAMARD
                     VRVILIKLADRLHNMRTMESMSADKRVRIASETLDIYAPIAHRLGLNQTYRQLQELAF
                     QYLRPWRHETLSKALLKARGNRRDVVERIQGDVEKSFAQAKLMVQVFGREKTLFSIYR
                     KMREKHLTFAQVSDIFGFRIVVSTLPECYLALGVLHQLYKPLPGRFKDYIAIPKANGY
                     QSLHTTLVSPLGTAVEFQIRTEPMHAVAEKGIAAHWMYKTKNSGATNAQEAQRLGAMW
                     LQSLIDIQDETRDATEFLEHVKIDLFPDAVYVFTPKSKILALPRGATPVDFAYAIHSD
                     VGDHCVAAKVNGEPVALRTELRSGDVVDIVIAPGARPNPGWLNFVRTGRARSKIRHHL
                     KTMEQEESQDLGLKMLAQALRAEGMEMPLDDASGALAPVWQPLLRWSGNRSRDELLTD
                     IAVGRKIASIVAKRLSRLLTERGVRPDAVTLTMGRYGADEAPSQGMVLIDGSEGASVQ
                     LAACCHPIPGDSILGYLGRGEGLLVHTAECSVGKRLLGRDSEGWLKVDWAEQPTRSFE
                     TGVSLLLKNGKGVLAEVAASVAAAEADITHIDMGDQAASENAELKLLLVVRDRLHLAD
                     VIRTLKRSAAVLRVSRIKP"
     gene            complement(33236..33439)
                     /gene="rpoZ"
                     /locus_tag="PKV56_04230"
     CDS             complement(33236..33439)
                     /gene="rpoZ"
                     /locus_tag="PKV56_04230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOX67050.1"
                     /translation="MARITVEDCLVHIPNRFQLVLAATYRARMLSQGHAPKIETKNKP
                     GVTALREIAAGEVGIEMLRKVPV"
     gene            complement(33482..34105)
                     /gene="gmk"
                     /locus_tag="PKV56_04235"
     CDS             complement(33482..34105)
                     /gene="gmk"
                     /locus_tag="PKV56_04235"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOX67051.1"
                     /translation="MQQAGSLFVVAAPSGAGKSSLVKALLELDSHLGVSVSHTTRAPR
                     GQEQDGREYHFVGESVFRSKIAAGEFLEWAEVHGNLYGTSRTAIESRISQGHDVVLEI
                     DWQGALQIKRLFPSAVLVFILPPSYEELAQRLNRRGEDHPDVIAQRLVNARLEVAQAR
                     NFDFVIINSLFETAVFDLKTIVHSQRLRYAVLAKNKPAVFRALDLLD"
     gene            34315..35709
                     /gene="prsR"
                     /locus_tag="PKV56_04240"
     CDS             34315..35709
                     /gene="prsR"
                     /locus_tag="PKV56_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOX67052.1"
                     /translation="MTSDSRALLLIVEDDPALQKQIKWSLDRFESVTADDRATALAQF
                     RRHTPAVVTMDLGLPPHPDSVDEGFKLLAQMIELNPDVKVIVLTGQNDQSNALRAIDL
                     GAYDFLAKPFDPDVLGLIISRAFNLFELQSENRRLREMQQPDLQGGLITRDQQLLRIC
                     RTIEKVAPSNATVLLLGESGTGKEVLAQSLHHASRRAGKFVAINCAAIPENLLESELF
                     GYEKGAFTGAAKTTPGRIETAHGGTLMLDEIGDLPMALQAKLLRFLQERTIERIGGRQ
                     EIPVDVRVVCATHQDLGEFIKTSRFRQDLYYRLAEIVVDIPPLRARQGDAVLLAQAFL
                     RRFAREQNRSAMGYTDDALAAIEAYGWPGNVRELLNAIKRVSIMAEGNRVTSEDLGLP
                     NVAGADSGEDAGLDLRAVRDKAERGAIVAALARSDGNLVKASEILGVTRPTLYDLMRR
                     LTIQWKGVEPPSAK"
     gene            complement(35720..>35980)
                     /locus_tag="PKV56_04245"
     CDS             complement(35720..>35980)
                     /locus_tag="PKV56_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX67053.1"
                     /translation="SPTARQLDARAISRIKEGSFDFIQLDATAYPGNSGGPLFDLETG
                     AVIGVVNMVALKGTRESALSHPSGISYAIPVEFVLRMLAAAR"
BASE COUNT         6616 a        11274 c        11648 g         6442 t
ORIGIN      
        1 atccgcctta gcctgacctt cgtcgacgcg actcaggtgc gagcaagcgt cgggccactt
       61 caagttggtg cgcaacgcat ccctcgacca caggaaacca caccatgagc tcatcgacga
      121 cgcaaacgct aggcaagacg ctgggcacct tccagctgtg gggcatcgcc gtcggactgg
      181 tgatctcggg cgagtacttt ggctggagct acggctgggc ttcggccggc accctcggct
      241 tccttgtcac gtcggcgttc atcgcgctga tgtacaccac cttcatcttc agcttcaccg
      301 aactgaccac cgcgattccg cacgctggcg gaccgttcga gtacagccgg cgagcattcg
      361 ggccgactgg cggctacatc gccggctacg ccacgctggt cgaattcgtg ttcgcgccac
      421 cggcgatcgc gctggccatc ggagcctacg tcaacgtcca gtttcctgca ctggatccca
      481 agtgggttgc cgtcggcgct tacatcgtct tcatggggct caacatcgcc gggatgaaga
      541 tcgctgccag cttcgagctc atcgtgacac tgatcgccat cttcgaactg ctggttttca
      601 tgggcgtcgt ggcgccggcc ttctcggtcg ccaatctcgt caaggggggc tggtcggggg
      661 ccgacacctt cagcatcgga tcgatctcgg gcatcgtcgc ggcgatcccg ttcgcgatct
      721 ggttcttcct ggcgatcgaa ggggtggcca tggccgccga agaagccaag acgccgcacc
      781 gctcgattcc gatcgcctac atcggcggca tcctgacgct cgttgcgctg gccaccgggg
      841 tgatggtgtt cgccggcggc tcgggcgact ggaccaagct tgcgaacatc aacgacccgc
      901 tgccgcagtc gatgaagacc gttgtcggcg aaagcagcgg ctggctgcac atgctggtgt
      961 ggctcggtct gttcggtctg gtggcatcgt tccacggaat catcatcggc tattcgcggc
     1021 agatcttcgc gctgtcgcga gcaggcttcc tgcccagtgc actgggccag atccatccga
     1081 agctgggcac gccgcacgtc gcgatcgtgg ccggtggcgt gatcggcatc gctgcgatct
     1141 tcagtgacca gttgatccag atcggcggca tgtcgctgac cgcgaacatc gtgaccatgt
     1201 cggtcttcgg cgcgatcgtg atgtacatcc tcagcatggc cagcctgttc aagctgcgta
     1261 tcagcgagcc gcgcctcgag cgacccttca gcgcgccggt gttcccgctg tttccggcgt
     1321 ttgcgctggg cgccgccctg atctgcctgc tgacgatgat ctacttcaac ccgatcgtgg
     1381 cggggctgtt cgtcggcctg atgatcgtcg gctacctcta cttcctgacg acggcaggtc
     1441 gccgtggcga ggcgatggaa gccgcccgcg cgtactgatc cccgacgggt ctgcgtccct
     1501 catgcccctc tacagccgcg ccgtaggtgt ccgcaacttc cggttcgaca gcctgaagac
     1561 gctgctggcg cgcgcgacgc ccgagcgttc gggcgatcgt ctggccggcg tggcggccgc
     1621 cacggcagaa gagcgcgtgg cagcgcagat gacgctggct gacgtcccgc tgcgtcagtt
     1681 cctgaacgag gcggtggtgc cctacgagga cgacgaggtc acgcggctga tcgtcgacag
     1741 ccacgatgca accgccttcg ccgcggtggc gcacctcacg gtcggtggct ttcgagagtg
     1801 gctgctgtcg gacgaagtcg acagcgcggc cctggccgcc ctgtctggtg gcatcacgcc
     1861 cgagatggct gcggccgtct cgaagatcat gcgcaaccag gatctgatca tggtcgcgcg
     1921 caaggtgcgg gtgcgcagcc ggtttcgcaa caccatcggc ttgcccggtc gcctgtcgac
     1981 gcggctgcag cccaaccacc cgaccgacga caccgccggc atcgcggcca gcctgctcga
     2041 cgggctgctg tacggcagcg gtgacgcggt gatcggcatc aacccggcca ccgacaacgt
     2101 gccgcaggtg atacggctgc tgcagatgct cgacgcggtg atcgagcgct acggtatccc
     2161 gacgcagtcg tgcatcctga cccacgtgac caacaccctg caggcgatcg aacgcggtgc
     2221 gccggtcgac ctggtgttcc agtcgatcgg tggaacgcaa gccaccaacg ccagcttcgg
     2281 gatcgacctg gcgatgctgg ccgaagcgcg tgcggcggcg ttgtcgctga agcgtggcgc
     2341 gctgttcgaa gacgaaaccc gatcggagcg tggcgacaac gtcatgtact tcgagaccgg
     2401 gcagggaagc gcgctgtcgg ccaatggcca ccatgggtgc gatcagcaga cgatcgaggc
     2461 gcgcgcctac gccgtggcgc gccacttcga gcccctgctc gtcaacaccg tcgtcggatt
     2521 catcgggccg gagtacctgt acgacggcaa gcagatcatc cgtgccggcc tcgaggacca
     2581 tttctgcggc aagctgctgg ggctgccgat gggatgcgac atctgctaca ccaaccacgc
     2641 cgaagccgac cagaacgaca tggacgtgct gctgaccgtg ctgggcgtgg cgggctgcag
     2701 cttcatcatg gggattcccg gttcggacga cgtgatgctg aactaccaga ccacctcgtt
     2761 ccacgatgcg ctctacgcgc gacgcgtgct cgggctgcgg cctgcccccg agttcgaggc
     2821 ctggctggaa cgcatggggg tgttcagccc cggcggaaac gcgcggctgg ggcgtcatgc
     2881 cagcgggctg ccgccggctt tcgaacacgc cttgccgacg cccgcgctgc gctgacgcgc
     2941 gaccggtatt cattgcgatg cccagcgaat cggaccccgt gaagcccacg aatcccattg
     3001 cggcggagat cgagtccccg gtcgtcacca acccctggag cgtgctgaag caattcacgc
     3061 cggcccgcat cggactgggc cgcactggag tgagcctgcc gacgtcggca cagctcgact
     3121 tccagctggc gcacgcgcag gcgcgcgatg cggtgcatcg accgctcgag gttcaggcgc
     3181 tgtgctccga tgtcgaggcg ctgggcctcg agacgctgcc cctgcacagc gccgcgacca
     3241 cgcgatccat gtatctgcag cgccccgatc tcgggcgcca gctcgatgtg tcctcgcgcc
     3301 agcgcctcga agcccttcgc gcacgccggc agtcgcaggg cggccagaag agcgatctcg
     3361 ttttcgccgt cgtcgatggc ctgtcggcgc tggccgtgca gcgtcacgcc gcaccgctgc
     3421 tggcggaagt gctcgcgttg ctggatcgat cggtcgcagg gcaggtgccg cggtggacgg
     3481 tcgctccggt tgcggtcgtg gagcaggggc gtgtggcgat cggcgacgag atcggcggtt
     3541 tgctcgatgg ccgcatcgtc gtggttctga tcggcgaacg tcccggcctg agctcaccgg
     3601 acagcctggg cctttacctc acctgggagc cgcagcccgg tcgccatgat gccgagcgca
     3661 actgcatctc gaatgtgcgt cccgagggcc tgggctaccg cgatgccgcc gcgcgtctgg
     3721 tctatctgct ggaggaggcc cgccggcgcc agctcacggg cgtggggctc aaggacgaaa
     3781 gcgattccgc ggctgatgtc gtttcggctc agccgaattt cctgctgcgg ggcacgtgaa
     3841 cccgggaccg ggttcacgcc ggttcgccgg catcgaggct gtcgcgcaac ctgccgacga
     3901 gattcagatc gaggacttgc ccaaggtgcg gcccgatcgc tccaccgctt cggctttcgg
     3961 cggggcggca tggtaggcag cgaggtaggc atcccgaaac ttcttgccgt cgaatcgccg
     4021 ctgggcttcg acgcgcgcag ccgaaccgag ccgctgccgt tcttccggcg agtcgatcag
     4081 ccgtgcgatg caggccagaa aggccgggta ggtgccgcag cagtgtcccg tgacgccttc
     4141 ttcgatcacc tgccggtggt acggcgtgtc ccacacgaca cacggcaagc cagcggccat
     4201 ggcttcggtg aggaaaaccg ggaagcccag ctcgccgccg aatgccacat acacccaggc
     4261 cgcagacagt cgactcgcgc gctcggaggc gtccgtgaca tcgaagacag cgacgttcgc
     4321 cgccgacaga cgggccagcg agtcgtcatc tgccgtgccc agccagttgc agctcacctc
     4381 gagcgactct tcacctagca gcaccgccag ctgcgcaaac atggcggcgt ctgcgcggtt
     4441 gcggcttctg ctgccggtga tcagcagcgg gcgacgcgcc tcgtgccgag tggcacaaaa
     4501 gaatgccgcg tccaccgggc tttcgacgat gggaatcttg tcgttggtga cgcgcttcag
     4561 tgcggttgca tcgaaggacg agttcgcgat ggttcgttgg tccgacagtc gaaccaccgg
     4621 cttgagcagg aacaccgtca acgcgctgac tgcggtgatg ggtttgagca ggcgcgaacc
     4681 gtgcggcgag aagtagagcg tctgcgacaa gccgtgaaac cgcttggcgt acgtgcccac
     4741 gaggctcggt atgaatccgt gcaggtgcac cgagcgggtc ggttgggctt cgatcacggt
     4801 caacagggcg cgcagcgagc ggttgatgcg tcgcagcggg cccgtttcac ccggcatcaa
     4861 caccagacgg atggtggggt ggaagcgggg aagcaggtgc cgatgggcgg gtgcgtcgac
     4921 caggatgacg gtctgctcca tgcccagttc ggcctgaacc tccgtcatgg gcccgagcag
     4981 ggaaaagacc gcatcggtga cgacacccgc gaggtggacg atgtggtgac cgtcgggcag
     5041 tgatgtcttc atcggtggcg agatcgcgag cccgaaatgc aagcagaggc tcgggtcatc
     5101 gagagccgtc gcgcgccagc accaccttca ctgtcttcaa cagaatcgcg gcatcgagcc
     5161 atatcgttcg ctgctcgacg tacctgaggt cgagcgccac ccgctcctcg taggtcgtgt
     5221 cgctgcgtcc gctgacctgc cacagtcccg tcatgccggg cagcacgctc aggtagtccc
     5281 agcggacctg tccatagcgg cacaactcgg ccaccgtgat ggggcgggga cccacgaggg
     5341 tcatctctcc gcgcagaacg ttgaagagtt gtggcaattc atcgaggctg gtgcgtcgaa
     5401 ggaagttgcc cacgggtgtg atccgggggt ccttgctcag cttgttgtgg agtgcccact
     5461 cgctgcgcgc gaccgggtcg tcccgcagca catcggccag catctgcgct gaatcgcggt
     5521 acatggtccg aaacttcagg caccggaaga gttttccgtt cagcccgact ctgtagtgcc
     5581 cgaagaagac gggcatccca tcgcggtgcg cgaccagcag cgcgatcagc gccatcgcgg
     5641 gcgcgaacac gcacagcagc agcagtgcgg cgagcctgtt tccgaccacg taggccgtat
     5701 ctctcaagct ggctcgattt gtcgggcgca gcagtgcgtg catgtcgctg gaggaggcgg
     5761 tagcagtaat cattcaaccc tcctgagtcg ttggtggccc ggacgtgggc tgcagatgtc
     5821 gagacattgc tgcaatgcac caagaaaagt tttgcatgct ttcgcaaatt ccggcctctt
     5881 cattgcacgg agttacgtgt ttacgttatt gacacagata cttttctaaa catcgaagtt
     5941 ttgtattgca atctgcgtgc agtggtgctg gagcagcacc gcgaatgccc gacaagcgcg
     6001 gatccccgtt cgctccggac gcattcaccc cttttggcgt gtggcctcgc accgcatggg
     6061 gcgccgcaac ttcgatggga gcagtcgtgc acgaatgccg cgggtcgaat cagttgcgca
     6121 ccgcgtgaag atcctcctgg tcgcgacgcg cagcgtcgag ggcccgcgca ccggcaggaa
     6181 ggtggtgctc gcaaccatcg tccgcagttg caaagcgctg gggcaccagg tcgacatcgc
     6241 ggtcatcggg ccctcggaac catccacggg cggcatctcg atccaggcgc ccggcttcct
     6301 gcgcgtgggc cacaacttcg tgaggcactt cctgctgggc cggaagagtc tcaacgaatg
     6361 cctttactgg agccggcaaa cgcggcgtcg gattgcagac ttggtcgtca gcggtgccta
     6421 cgacatggtg gttgccgaca tgctccgcag tgcgcagttc tgttcagggc tgaaggtccc
     6481 ctggattctg gatctcgatg atctgctttc cgatcgctat gcgggtctcg ccgcggcaga
     6541 ggatgggcgc atgctgctgg gctatttctc gcatcgattg cccgtgtcgc tgcgccggtc
     6601 ggctgcatgg atcgcgcacc gtctgctgaa gttcgagtcg cgtcggatcg ccgcgcagga
     6661 gcggcgattc ggcttgctgg ctgatgcggt cacgctggtg tcgtcgatcg aggctgccaa
     6721 gctggcgcgg cagctgggac gcgcagtgca cggcaccgcc atgggcatcg atgtgcccga
     6781 tctcgcgccc atcgatgcct ctcgtcgcca cgctcgtgcc gtggcgttca tgggggggct
     6841 ggactaccag ccgaacctcg cagccgtcac gcactatctc cgttgcatcg cgcccgaact
     6901 cgagcgcctg ggatgctccg ttgcacttca cgtgctcgga catgccccgc acgagctgcg
     6961 cccgcccgat cccgtcgggc tggcgaccta tctcggctac gtcaacgacc tggaatcgac
     7021 gctggctcga tacacgatct tcgtggcacc gatcctggct gacggcggaa tcaagaccaa
     7081 ggtgctggaa gccatggcga atggcctggc cgtcatcgcc accgagcagg cggtggcggg
     7141 tctgggcatc acgccgggca aggagtgcat cgtgacccgc accccggggg aattcgcccg
     7201 agcggttcaa gcactcatcg acgaccctga tggttgccgg cgactcggcg ccgctgctca
     7261 gcgctttgtc ggcctgaact tctcccgctc ggtgctggag atcaagtggc agcagctgct
     7321 cgaagacgct gcgtcacact ttcaactcat ggcagcttcg tcctgcaacg cgggcacatc
     7381 gaagccgact cgctgaatcg cgatgggcag ggtggcggcg ggtgcgcctg ccacgagcag
     7441 ctcccaggtc gaggctcccg cctcgctctt ctgcagcagg gtgaatccga gtttgtcgta
     7501 gtgatcctcg acaagcttgt tcttctcggt cggcaggtag ttgccgatga gtcgcagcac
     7561 gccccgctcc gccgcctggg acatcagctc ctgcagcacc gcgacttcca cctttcggcc
     7621 cagcacgcgg cagctcatca gccaggtgtc gatgtgccag tcggtgccct ggcggcggca
     7681 gatcagcacg ctgatcatgc cgttgtcgcc gaaggcatcg gtgagtcgga cctgcagcgt
     7741 gaaggcgtcg ggatcgatct gcgccgcggc gacctcggcc tcggtgtagc gccgcgtcgt
     7801 caggttgaac tgattcgact tgttgatgag ctgcgcgatg cgagcgcgcc cggtgtcatc
     7861 gaagggttga agcgtcatct gcatgttcag cgatgccagg taagcatcga tgtcgcccgc
     7921 ctgcttctgc agcgtcacgc ggcgggcgtt gtcctggtag aaattcgcac gctgccgatc
     7981 ctcgccggaa aaagcgatcg cctcgaagta gccggcggcc agcagcgtgc gcgcgtagag
     8041 cgcggcgttc tcgggcagct cgggtacggc cacctgaggc agcatgcggc ggaccagccc
     8101 gcgctcgacc gggttgtcgt ccaggaagac catcgcatcg agaccgagcg acaactcctc
     8161 ggcaatcgcc ttgatgttgg tggccttgtc gttccagttg gcctggaaga ccgcgaagtg
     8221 ctcttcgcga agcagcatct cggggtgctt gcgaaagggc tggcgtgcga tctcgtcggt
     8281 gttcttcgac gacacggcca gaaccacgcc gcgatcacgc agggcgagcg cggtgcgctg
     8341 gacctgaagg tgcgcttcac cggtggcatc gccctggccg atcacgatgc cttcgacgcc
     8401 gtcgtcgccg atgacgcctc cccacagcgt gttgtccagg tccaggatca ggcacttgcg
     8461 gctcttgccg cgcaccgacg cgatcaggcg gcacacgtgg tcggcgtaga gcggaaggaa
     8521 gtcactcgcg aacggcagct tcgccatgtt ccagagtgtc gggtcgtgcc agtcggccaa
     8581 cccgacggtc tccgcgatgt gggccacatc gagcagcagg tcttcggtgc cggccacgct
     8641 catcgcgatg ccgcgattga tctcgtcgat cagttggcgc ggggtgccgg gaagcacgag
     8701 atcgaagctc ccgaacgtcg actcgacggg gcgcggcagc gtctgcatga tgcagatcgc
     8761 cttgctgttg ctgtgcaggc ccctgcggat catctcgagg tggccgagtg cagcctggac
     8821 gctcgcctgg gctccctgcg ggtcgcccgg cgtgaaggcc agcggcaggc cacggtggtc
     8881 gacggccacc agcaccgcgt cgcacttcgc ctgatgaatc ggcgaatcgg ccgacagcgc
     8941 ggcctgcatg acctggtcgt agtcggcctc gatgcactcc agcgcgatgc cgtggcgggc
     9001 tgcactggcg atgagcgcgg gcacgatgaa gtgcgacgtc gcgttgctga tgatgccgat
     9061 gcgaaacgcg gtcagcggtg cgagcgactg ccctgcatcg cgcgccttgt cgatcacctt
     9121 ggccaggcgg ttcagctggt tctcgtccag cgcgtggttg gccaggtgct gcaggcgacg
     9181 gcccagatcc gaggggtcgt taagaaggtg acggcacgag gcggaaaaat cctcgggtgc
     9241 cgccggcagc catgccagtt gcgtatagag agcgttgctc atgggccgat ggcctcctcg
     9301 aggatcagcg acgccacctg ctcgggcgtc tggaacgcag ccttctcgcg ctgcatggca
     9361 aggaagcgct catcgaaggc cgcgagcatt cgcgtttccg tgtagccggg cttcacggat
     9421 cgcacacgca gccagggatg gtcggcggcg agcagggcca gcatgccggc catgccgtgc
     9481 ttggcgatca cgtatgcacc catgcccgag gcggcacccg cggcgccgtc tcccatggct
     9541 tgcgtcagca cgcccaccac cgaaccttgc ttgtgcctgc gaaagcagcg tctgaccagt
     9601 tcggcgagca gtcgctgggg gcccatcacg ttgacctgcc actgctgccg catctcgtcg
     9661 gtggtgatct ttccgaacgg aaccggcgaa agaggcgggg agcctgccag caccacgccg
     9721 aaaaggcgcg gtgcggcctc tagggtcgcc accgcagcca ggatggaggc gtcgtcgccg
     9781 aggtcgagcg cgagtgcccg gccaccggtc cgctgcgcca gcgactcggc tgcagccgcg
     9841 ttgcggtgat agcccacgat gggggtgtac ccacgcgcgg cgagctgctc gcacacggcc
     9901 gagccgatgc caccggagcc accggaaacc aggaaccatc tttcgtcggc tgcacggcca
     9961 acgtccgttt cagtgtccaa cgaccacctc cgccgtgccc ttggcgagca ccttgccgtt
    10021 cgcacgaacc gtgaccttca gcgccaccat cccggtcgac tccgatgctt ccgtgaccac
    10081 gccttcgacc tgcgcggcct gcccgacgta gagcggcttg cggaaatcca gcgcgatgcc
    10141 gacccacacg ctgtcgcggc ccggcagctg catcccgatc agctgggaaa ccttggccac
    10201 catcagcgcg ccgtaaacga cgacgccgtc gaaacccttg gtgcgcgcga atgcatcgtc
    10261 ggtgtgcagc gggttgaagt ctcccgagag ttcggcgaac tgctgcatct gcgctgccgt
    10321 gaccgagaac tcgacgcagg cgtgcaagcc ctcgaccagt tcaccgagcg ccagggcacg
    10381 cgaggcaggc gcgctgctca tgccgtcttg gacgcgatca gcgaggcgaa ctcgccgacg
    10441 ttcttcaatc gcccgacttc tgcagccgaa aacttcaggc cgaacttctt ctcgatggcc
    10501 accacgagac ggatgtggct gaggctgtcc cattcatcga cgtcgtcggc ggtcagctcc
    10561 ggcgtcacca cgatgtcttc atcgtcgaac acatcgtgga atatcgcggt cagcttggca
    10621 tacagggcat cgtggctcat ggcgggggtc cttgaagaat cgggagggga aggatctgct
    10681 cgaaggctgt ctcgctggcg ctcagtgcgc gctggctgac tgcgatgggg gcagggattc
    10741 gctggtcttg atgacagcgg ccagacgctg cgcccagctc gcggtcacgc gcggcttcgg
    10801 gtgcacctcg tcgctgaagt cctcatctgc atgtgcatcg tccatcctga ggaaggcgac
    10861 tccggcgcgc cccttcagtc cgctcagcaa ggaatcgagt cgcgcgcggt agtcggcggc
    10921 aagtggagat gcgctggtgt gccaaccggg aatcggcatg tcgaccagga tcacccggcc
    10981 tccttcggcc aggatgccct cgaccgtgcg ctgcagcagc cggaacggtt gagcctccag
    11041 cgagtccgtc gagcccatgt agtcacgcca gaacgcgagg tacctgcgct tgtcggcgtc
    11101 accgatcacc accgcattgc gctggtcgtc cgtcatcgac gccggtctgg acgccatgaa
    11161 atcgcgcagc gagcgcgtca ggtcgcgggc cgtccggtcc agcacgaggt acgggtgtat
    11221 cagcagcacg cccgtgtcga gaagcatcgg cggcagcacc gggacggcgc ccgcttgggt
    11281 tcgccggtag aagccgtagc gatagcgttc gatgtcgatg tcggtgtcgc ccgcatgccg
    11341 atccggcgtg ttccagcgag cctggtcgga agcgaagacc ccgtaccaca ggccgagcac
    11401 gtaggtgttg tgtcggcgtg actcggggct ctgcacttcg cggatcagat cgacgatctg
    11461 gctcacctgg ccgatgttgg atccgcccac cgacaggttg tgaacctcga cctctggcag
    11521 cagggcctga acctgcgcct gcctgaagcc gacgagcatg ttcgacgcgc ccagcagcag
    11581 caccttgtcg gagggcgtgt tcaaccggct gcgcgccatg aagacgtact tcggctcggt
    11641 caggaacagc gaggacgaag cctggctcgc atcgagccgc aggccgccct cgaagcgcgg
    11701 aaccagcagc aggctgatgg tcagaagcag cgcatacagc gccatcagcg cggcgatcaa
    11761 ctggaggatc tgcttgatga cgagcttcat cagaaggccc ggtagacgaa ttccggcgcc
    11821 ttgtgaaaga acgaggcgac ggtcacgatc agcaggatct gggaggcaag cgacaggttg
    11881 cgaacgatca caccgccgga tcgttgcgtc atgacccgcg cgatcggtcc cagcggcaac
    11941 atgagcgcat cccaggcgaa gaacgcagcc gctgctgcga gtgcgagccc gaggtagcac
    12001 gctgcgatgc cgacgaagcc gagccggcca gcaagccatg acaactcgcg catgtccacc
    12061 cagaggcagg tcagtgctag cgcgaagtag gccaccgtga ggccgcggca caggtagatg
    12121 aatgccgtgc gctcggacaa tgccttgtat cccttcttgc ccagccgctt cgtcatgaac
    12181 acctgccaga gcttgttgat gctggcgccc gtgcccatca gcaagccata gacgacgaac
    12241 accgaggtcg tgccgtgcca gacccccatg atcaggaacg tcacgaagaa cgccagcaca
    12301 ccgagatacg gaccggcctg gggcgaggtg aaacggctgg ccagcgtctt catcaacggg
    12361 ttgaagacgt aggtcctgaa ccagtcggac agcgtcatgt gccagcgcga ccagaagtcg
    12421 aggaagctcc tcgcggtgaa gggccggttg aagttctctg gcaactcctg gcccatcaac
    12481 gtgccgacgc cgatgacgat gtccatgtag ccggagaaat tggcgtacag gtagaccgtg
    12541 tagaagaccg ctgcagccac gaacaggccg cacatcagcg gctgtgcggg cgccagcgca
    12601 gggtccagca accgacccga catgctgctg aacaggtaat tgaagatcgc cgacaccacg
    12661 ccgactttca cgaatccctt gatcaggcgc gcgaacgcct gaaacaccgt ctcttcatcc
    12721 agcgcgagtg cgcggccctg ttgcagggtg tagtcaccgt atcgctgaac ggggccggcg
    12781 atgaaggaaa gaaagttgca cgtgtagttg acgaagtgca gcggtgcgat gcgctcgatg
    12841 agttcaccct gcttcgcgtc gatcatcagg tgcagcacgc ggaacagcac gtaagacagt
    12901 ccgatgatca ggtaggggaa cggcagtacc agcgattcgt ccaggaaact gaatttcttc
    12961 agcacgacga agctcgcgat cactgccacc gctcccatcc acaggcttgc cgccgagcgg
    13021 ctgcgtcgca cgccctcgat ggcgacatag caaagcagca gaaaggccgc cagcgggccg
    13081 acctggccga tgctgtcgac gtggctgccg atgaacacga ggctggcgag cagcagcacg
    13141 ccgctgcgac cagctggtga cggcagcgcc tgggtgagca gcaccacaac caggatgaag
    13201 gctatgaaga tcggcgaggt gaaggccacg gtcgcgttcc tcagtcctgc ttgaaccgat
    13261 tgagtagcgg caccagtgcg tcacggaaca cccggtagcc tgcgggcgag atgtgcagct
    13321 tgtcggccgc gaagtactct gcgcgaggtg cgccttccgg gtcggcgagc agcgcataca
    13381 gatcgagaac caccacgtcg gcgcgaccct gggttgccga cttgatcagc gtgttgagtt
    13441 cgatcagttg ccttggatcg accggggcct tcggatggtt gcgtggcggc aatgtgcaca
    13501 gcacgatcgg cacaccgggc gacgagcgct cggcccggtc cagcatctcg acgagattgc
    13561 tgcgcgtttg ctgaatgtcc tgcaatgcgc tcagatcgtt ggtgccgctc agcaggacga
    13621 tggaccgggg atgcaagtcc agcacatctt ccttgaagcg aaacagcagt ccgcgcgaga
    13681 cttcgccgcc gatgcctcgg ttggtcaccc gcagatcggg gagatccttg ctcaggctgc
    13741 tccagccgcc gatcagcgaa tcgcccacga acaccacgct gccctgcctt cgttcgcgct
    13801 cgagccagaa cgccttgcgg ttgtcggtca tccagccgaa gacgcggatc acgcctttgc
    13861 cgggccaatt cctggcgtct gcagggaaag gtgtggggcc ggatgccggc tcggtcgatg
    13921 cagttgcgga ggacggttca gcagccgccg tgaggtcgat cgcccccaga ctggcagcca
    13981 cagaaagaag tacaagaaat agctttgcga gatggttcat gttgcccgac accaggaacc
    14041 tgccgagcac gcagcgtgcg ggccgtaggt tcaccgcatg gccctgccgc gccactcgat
    14101 ggatcccggc aagaacgtga gagaagagtc gcatccggct tggaagacgc ctgcgactgt
    14161 gtgtgtcaac caacgatcga ttcgctaggg actcatgcgt tgactgtgcc gcaactcggc
    14221 gtaaaccctg caggctttat cgctaccaaa tgttgcgatg ggttcaatca cctcggtcac
    14281 tgcacacaat gcatgcggct cggagcgggt ggcgggcgcg acgcgttgct gcccgagctg
    14341 attcagttcg acgttctgcg gttcgtttct tgagactgat gtccggcagc agggccggat
    14401 gtcgtggaat tggagtgggc aacctttgca acaagagagt tcgagcccgc atttcgcagc
    14461 gcaagcgagc ccggcgcggc atccggcgat tcttctgctg acctacatct cgccgctgca
    14521 gcgttggggt tcggcgaagc gatcgcgatt cctcatcgat gcgttgaagc agcatggcgt
    14581 cgttgacgtg ctggttctca gcttcgtcga catcgcagat gccaggccga cactgacggt
    14641 gaccgagatg gacggggtcc aggtgatgga actgcaactc gtgcaacgcg gatggcgtag
    14701 ccgaccccgg ttcgacctga tgtcgggtga tgtcagcaag caggtggcgc ggcacattga
    14761 tcttgcgtcc tacgacctgc tggtcagtcg ctacgtgcga ccggccatga agctcgatct
    14821 gcctgaaggg gtgccattga tcgtcgactt cgacgatgcc gtgtacgacc cgccgtggcg
    14881 ggcgctgcgt acgttgaaaa gctgggttgg cgtattgctt cgactgttca acgatcgggt
    14941 catcgtgcgc acccgcctca agggcgcatc gtggcggcgt gcgcactacc tgttctgccg
    15001 cgaggccgag cgccaggtgt tcggatggct gccgggcgcc gtgctgccca acctgccccc
    15061 agccgccgat cgtgccggac cacctgactt ctcgccgccg gaacaaccgg ccctcatgtt
    15121 cgtcggcctg ctggactaca tgccgaatct cgatgcggtc gactggttcc tcgatgccat
    15181 ctggccgatc gtgttgggtg aagttccgca ggcacgtttc ctgatcgtcg gcagtggtgc
    15241 cgatgaactg ctggagcgct ggcgcaggcg cccgggcgtc gagacgctcg gctttgtcga
    15301 ttcgctggcc gacgcctatg cgcaagccac cgcctgcgtg gtgccgatgc ggtccggcgc
    15361 gggcacgaac atcaaggccc tggagccgta cctttacggc cgcatggtca tcgccacgcc
    15421 gttggtggtg gaggggcacc atccgctgtt caatgcgggc caggacattc tcgtggcgcc
    15481 cgatgccaag gggctcgcac gtcactgcat cgacatgctt gtggcgcctc ggcgagccag
    15541 cgaactcgct cagcgtgggc accagcgcat cacgtccacg ctgaccgaga aaaactttca
    15601 aaggatcctg ggcgatgcgg tcgccttgct gctgtgcccc cgcaggtgac tgtctcaatc
    15661 ctggcggtgc aaacgtatcc ccgcacagtc gtcaagtcgg tggggtgaag ctcgctatct
    15721 atcggcgtcg gtggtcttct cctgcatcga cacacggtct cttcaggctg gcgcgttgct
    15781 cggcagcacc cgctccagat tgacgaagga tgcctctctg acagcgggaa gatgttcccg
    15841 tacgcactgc gtcaactctt gcaaacggtc cacgaactct tccaccatgg ccgtcagttt
    15901 gctggcatct gcagcggcag caggggtcag cagaaagcgt tcggggtatt cgaagtgcgc
    15961 gaacagtccc tggaacttgt cctggtaggt caacgcggcg accggacgac ccatacccag
    16021 cgttgcgatg gcgagatgca tgcgaccggt cacgacgcca tccgtgaagc tcgccatggc
    16081 cttgagctgt gctgcagaga accgagttgt cgggtagttc agccgcggtc cgagttcgtc
    16141 agccagtgcc agtgcaatcg gtgcgagaca gacgtcgtcg ccatcgtgcc cacggtagtc
    16201 gtgagacagc agcaggatgg acacaggccg gcgctgcgcc acggcgcgaa gcgagctcac
    16261 cgcacttttc accagggcat tgatctgatc tgcacttgca tgccggaaaa gcatcgggtg
    16321 catgttgaaa ccgacgatga catcgccagc ggctcgccga cttgtcgacc aatgttcgac
    16381 agcctgcacg gtatcgccgc tgaagtctgc ctcgagcatg aaggcagagt ctgcaacgag
    16441 gcggcacgga gcattgcaga atgaatggaa gcgatcgaat gaaactcgat ccctcaagtt
    16501 gatcgccacg cgtttgctca ggccctcgaa caccggtttg accagactgc tcggtcgatt
    16561 gttgaagctg aagcccagga tcgagacgcg aacaccttgc cgggcagcga cgtcagccaa
    16621 tagcagcatc cggctggccg tgaccgggtt gtagtagcca tccagtacgt cggcaccgag
    16681 caccaccatt gcatcgggat cgaatgcctt gatctgggtc gcagcttcag cgagggtgca
    16741 gtcccacgcg ggcactgaag caaaggcgag ttggcccgct gccgcttcag cgcctggcgt
    16801 tgcagtgatc acgccgacct gcaggtcggc actgtactgt cgaagacggg tggtcaccgc
    16861 ttgcatcatg gcttcgtcac ctttggcccc gaccagtgtc cagggatcgc ttggcaatat
    16921 caggaggcgc cgcaacagcg tggattcgcc aggttccggc ttctcgcaca cacgatcagc
    16981 taattgcccc ttcagtgcgg aaatgtctcg ccatgcaagc aagtgccggc ggagtcgctt
    17041 caggtgttcg aggggcatgg tgagttgggc gttcagcgct ggttgtgctg gagattcagg
    17101 ccgcggcggt gggctgccgt cctggtcgaa ggaaccggag gcccgtctgc aacgctgcgg
    17161 gcgcgaagaa ctgcaggcag aggaggtaca cgctgactgc agcggcgcat gcgcccagca
    17221 acaccagtgg agcggaagct cccctcgcct cgagcagcga agcgacaccg agcgcgacaa
    17281 gggccatgaa agtgctggac agcacatagg gccacagagt cccaaacagc tgcgtcaggg
    17341 acacaccgat caatcgcctc acgagccaga agctcagggg tgtggtcgcc aactggctgg
    17401 ctacgtaggc ttggagaagg acttgaaggc cggcgtccct ggcaaggaac agggcactga
    17461 atgtcagcat caccttgaag atcatgaact gcagccccac gccaggccgg ccgatcgcgt
    17521 tgtagaccgt accgttgtag acctgcagga cctggacggc ccccagcatc gccacccatc
    17581 gcgtgacctc tgcactggcg acccaacgtg caccgaacag cgtgaccgtc agcgacgggg
    17641 ctaacgccgc aatcagaatg aacaccggca tcacgatcgc cgctgtggcc gcaatgctct
    17701 gaaaataggc gcgtatcaag cccggcctgt ccgcagacag tcggcagaag ccgttttgcg
    17761 ccacttcaag aatggtcgaa ctgaagacct gcatgagcgc ctggtagaca cgcacgccga
    17821 ccgtgtacag cccaagcgaa gctggcccca aggtggaggc caggaacagt tcgatgtatc
    17881 gcgtgttggc gaagtcgagc agggaagtcg agacccgttt cgcaccgtag gccatcaagg
    17941 ggcgagtggc agcgaaatcg gtcttcatcg tgaggtgcca gctcggcctg atccacaaca
    18001 aggcactgat cacggcggcc gtggtgaggc tctgggccac cagactccag acgcccaggc
    18061 cggcaaacaa catcgccagg gccaccatgc tcgagaccat cgtggatgcc aggaccgtct
    18121 ttgcgagcca tcggtactcg aagcgtctcc tgcgcatggc caactgaccg aacgaggcag
    18181 cggtcagcgg catcgacagc gaaacgacgc gcaggatcgg gaccagttcc ggaagtttca
    18241 tgagcgctgc gatcagcggc gcgacaaacc agagcagacc gacgatgagc agcgacgcgc
    18301 ccaggttgag ccagaacacc gagttgatct gctcctgcgt gatctcttcg cgctggacca
    18361 gcgcctcacc caggccctgt tcggacagca ggttgagaaa ggcgatcacc acggtcgctg
    18421 ccgcgaaaag accgagctgt tccggagaca gccagcgtgc gaccaccatg aacagcagga
    18481 gtgaactcag ccgcactccc cagttctgga cggcggacca caacacgccc gatctgacct
    18541 tggcgcgcaa gtcgttgctc aatggatgcc cttgcgattg gccgagggag ggctcatgcg
    18601 ccgtggatcg tcatgatcga cccgacgtcg cgtcttgcag atctcggcac cagtgcatct
    18661 ggcgatgcgt agccgacagc caggtacatg atcacctgat cgtgctcggg caattcgccc
    18721 ctcgcatgtg cctccgcatc gacctcgggc gcgacgcacc agttcaggca gcaactcgcc
    18781 agtccgcgcg cctgcagagc gaggatcaaa ctcatcgcga acaagccgcc gtccacatgg
    18841 ggctcatgcc tctcggatgc gtcgaagaag gaacgactgt cggcacagat gaccagcagc
    18901 gttgacagca gatgaccaaa tccggaattg ccgttctgca aggacagcat ctgcttgatc
    18961 tgcgcagcat cgcggtagac atgcaccctg cacggttgtc gattgcatgc gctcgggctg
    19021 agctgggcgg tcgcaatggc ctcttgcaac aggggcagag ggaccgccgt ggaggagtac
    19081 gagcgcacgc tccggcggtc ctggcacagt cggtcgaata catcggctgc gtcttcggtt
    19141 ctgcgatagg cgtgtggcgt tgagaactgc ggcgcttcgg tcgtgtgact caggcaactg
    19201 ttcagcagtc gttgaatcag tggcgcatct tccgcgttcg cgggtgtcgc ttcaaggcgg
    19261 gtccggtagg ccctcaatgc ttcaatcgca ccgatgaaga ccgggtcttg catcgagaac
    19321 ccatgcgcct gccagcgctc caccagctgg cacgtcgcaa cgagcgggtc tcgaccgaag
    19381 tagcgcttcg tgcccgggat gcacagccct ttctcgagct tgtgaaactg gaagagcaat
    19441 tcggaactga gcttcgcata cgacggatcg cgctcggacc agaacatgaa acgcttggcg
    19501 cgaatgacat cgctcgcgaa gtagcggagc ttggcccaac gcttgacgtt ggttttccag
    19561 ccagccaacg tttgctggac gcttggaaaa ggtttgaccg cccggttgac cgatccgaga
    19621 agcagcgact tcagcatcgg ctcacaccgc cttcagtgaa atgccctggg gtttggccaa
    19681 cgcggcgccc cagcggatga atggtttttc gatgaaccgg tacagcaagt aactcagcgg
    19741 tatgaggagg atgacggcaa tggacgtgct gactgcggca atctgctgag tccatgctcc
    19801 gccgagcttc gctgcaatcc agccgaccag cgaaaagaga gtgatgtgta tgaggtacgt
    19861 cgagtaggag aactcgccca gccaacgtgc caccgaattc gatgtgacga attccaggaa
    19921 cctggagaac caggctaggt cccggacggc tcccttcgcc tgcgatgcgc gcagttgaag
    19981 gcaagcggct gccatgaaga cgccccatac agccacctct gtactcactg atcggggagc
    20041 caatccgctc atcagcaggg cgacgggaat cagccagaaa ccgatgctcg acagcacttg
    20101 caggaatacc cgcgagagga tgccgagcat gaagaaatgt atggaaagtg gaaggactgc
    20161 cgggtactgc cattgcagcg gaagcacctt cttgaacaac acccacgaag cgctcagaag
    20221 caagagcgtg agaagcctcg acatgcctgg ctttgcgagg ccagcgacca aaaacggggc
    20281 gacgaggtag aactgccact ccagcgacag actccacgcc ggcgcgagaa aagctgtcga
    20341 gctgaaaggc agccaggtat cgggcaccag gccgtgcagc aggctgacat gcagcagcag
    20401 gtacttgctg aactctgctt ccgtggcgac agcgcgagcc gctcgcatct cgtgctggaa
    20461 gacccagtca cctgcatata ccgagctgta catctgtgcc agcagcatgg cgcaggccag
    20521 cgcgaaccag tagatgggaa agatgcgaaa agcccgtcgc accagatacc tgccataagg
    20581 ctcgcgccgt tccagcaaca gatgtgaaat gacgaatccg ctcaggctca cgaacacgtt
    20641 cacggcatgg tcaccgctgg aaagaatggc cggcgcaatg ttctgaaggc cgcatagatg
    20701 caggccatga ccgatcacca cccaccaggc catgtagccg cgcagaccgt cgaatgcatg
    20761 aaagcccttg acgcccgtgc gctcacccat gcccgaccag agcttgcaag cgattgcctg
    20821 cagcagaaaa acgaatttcg gcatcacgtc cctcccagat tgaagggatc gagggcgagc
    20881 cgcgcctgcg aaggtacggc tgcgtcaagt gccgcgcatc gggtcgactt cctgaccagg
    20941 gcgcccacca gcgtgctgac ctgtgccgcg ttccactttt ctgccgcaga acgcgcagcc
    21001 gcggagagcc ggcgtcgagc atctgcgtcg tcgatcaacc cgaggattcg attggcaaac
    21061 gctcccgggt cttcatcttc gacgacgaag agcgcatggc cgtcgagttc ctcgacgcct
    21121 cgcgcccccg accgggtggt cacgatcggc atggccgatg ccatcgcatc aagcagtttg
    21181 atgttgactc ccgtacccac cagaacagga ttcacggcga tcatggccgc accgaaagca
    21241 tcctgcagat tcggcacgaa tccgagcttc acaactccgt cttgatcttc gaattcggcc
    21301 gacaccgcgc cggcgagcag cagctgaaat tctggtttcg ttcggcgaac gatcggcagc
    21361 actcgatcga tgaagtagcg tgcaccggcc acattgatcg ggttggcaga tccgagaaag
    21421 acggctgctt cgcgtcttga agggggcgct gcgtcagcga tatcgatcag atggccaacc
    21481 tgcagcaccc gggttttgct cccttcgaca cgcgccttga aatctcgcga ttcgctcgcc
    21541 tggatcgcga ggacgatgtc tgctcggcgg aacccggagt cctcatctgc cacgctggtg
    21601 gagaaccatt gaggtcgcat cccggccttc aggtagagcc ggtgtcgcaa gccgaagcaa
    21661 tcgtgcgtat cgagcacccg caggcagtcc ggtgggaaag cctcgagcgc tttcgaagcg
    21721 aagacatatt cgacgaacac cgcgtcgtac tcatgcagcg aatgcagggc tgccagttcg
    21781 gaattcgctc gctcgtcata ccaatcgtcg agaccccaga cgaaggcctg gtcgatgttc
    21841 atgaggcggc ccagctttcg tgctgcacgg gagagcaatc cacccctcgg tggttccagg
    21901 cgtgaatgga ggacgtgcaa cctgtcaggg ccgaagcggg cctgcatggc tcgcagatcg
    21961 catgactcca tggggaagta ggcgaagtga acttcgtgtc ccgctgcgat tacgctccgc
    22021 gtcagggtca tgatgcgcac gcggttgccg gcatccggcg gatcggtcgg caccgccgag
    22081 atgaacagca cgcgcaggct ttcggtcgtc accggatcac cccgagcttc agcagcgtgg
    22141 cacgcagcgc ctgcggccgc tcggccggca cgaaccgcca gaggatcagg cggttcccag
    22201 ccttgcgggg cagcaggacg accagcgcgg cccacaagcc gagcatccat cgagcctttg
    22261 gtgacaggcc ctgcggcgtc tgcagcgcgc gcagcacgtc ggtgaacacc gcccacgcgc
    22321 tgtcaccggc gatcgggtgc gtcgcctgcg cgagttgcag cgatgccagc cggtagggca
    22381 ggtagtgcag gctgaggtcg agggccttgt cggaaacctc gatccccacg cgtcgtgcaa
    22441 cctgccggac gtacgcgctg cgctggcgcc cgcgcacgac gtgaaggtgg aactgactgg
    22501 cgtcgagccg ggatgcggca ccatcgttgc gtccgtgcac gcggtactgg cccagcggcg
    22561 catcgatcgt cgcgacatcg ccgagaaacg gggcggctgc cacgcaacac gagtcgccgg
    22621 gtcgcccgca ggcttcgtcg agcggcagga tctggtctag gtaggcacgc gaataggcgt
    22681 tgccggagcc tggtggcgtc gggtaggtgg tggttgccgc agcccagcga cgcagttcgt
    22741 caggctgtgg cgcgccatgg aactggggaa acgtactgcc gagcggtcgt gcttcgccat
    22801 cgaccaccct cagccggaac tggaccttgc tgagcccggg tcgccagatc gcggcgatct
    22861 cctgcatcac cgagcgctcg agcaggtcgt cggagtcgag gaagatgacg acgtcgcctc
    22921 gggccagcag gtagccggcg ttgtaggcca tgtactgccc ggcgttggct tgcagcacgg
    22981 tctcgatccg ttcgccgtag cgggcgatga cttggcgcga cgcgtcggtc gaaccgtcgt
    23041 cgacaacgat gacctgcacg tccggccagt cgatcgccag ggcgctgtcg atggcctgtc
    23101 cgacgaactc ggcgtagttg tagttcggga tgatgaccgt cagacgcaac gcgtcaccag
    23161 ccatttgcgg cgtccggtcg gtgtcctgcc gggcgttgtt catgcgaggt cctgccttcc
    23221 gctcgcagca tcgccgggtg cgggccccgg ccacatggcg agtgccacgt aaagcaggta
    23281 gacggtttca ggcgtcagca ggtacccgcc catcaccagc cagttcgcaa acagcaccgc
    23341 gcggaactgg aatggagccg caaacctgct gaggcagtac gccacgaagc cgagacagat
    23401 gacaaaaccg gcgatcccgt actcgaacag cagcttggcc caggtggggt cgtgggaatc
    23461 gaacgccgcg acgtttttct ggatggtgcc cggcccatgc cccagccaga tggaccaggg
    23521 cgaactgtcg aagttggcgt cgacgagaaa catcggggcc acatagcgcg cgtaggcgct
    23581 gcttccgcga gcgctgaact caccgaatcg gttcagcgtg aaggccaggt tcagggggtc
    23641 acccgccagc gcgttgctgc cgaggatcag gaccagcgcc gtgcctaccc aggccagcag
    23701 cctgtgccgg aacgggaaga gcaggccgaa cgccagcgtc agcaggccgg ttccggaata
    23761 ggacagcatc agggccagtc caaagcacag catgcgcttg acgcgcttga acagcgtcaa
    23821 ctcgatcagc agacccaagg ccatccagaa ggaaaagttg gagggttcgc gacagaacag
    23881 gccgttggcc ttgaaggtcg agccgaccgg gatcgcactg ttgtagatgc cttgcgcgcg
    23941 gatcggatcc gggatcagct tggagatgtc gaacagccag ggcgtgcgga tcacgaactg
    24001 cgcatagaac tgcacgatgc cggcgatcgc cacgatcagc agcacattgc ctgcggtgcg
    24061 catcatccac agccattgtc cctgggtgtc ggtctgtcgt gcctgggtga acacgaatgg
    24121 caggtagatg gcgagcatca gcgacagcga cgagatcgat gttccgctgc cgccatcgac
    24181 gctggcattg agcaggaagc tcacgacgcc caccgtggcg agggcactga acatggcgag
    24241 cgacagcgcg tcgagtgcca gcctgccctc tagcagcagg accgccagca gcacgtaggc
    24301 aagcggcatg gtcagcccga gcgggaagga tccgatggcg atgccgaacc ggtcggtcat
    24361 gaccatgccg gcgaacaggg cggtcatggc ccagcgaagt gcgggcgtgg ggcgcgtccc
    24421 gcttgtcggc atcgtcgcgg aatccttcgc gggcggcgcc tgcgtatctc gcgcggcctg
    24481 ccatcggaac agttggagtc ggataccttc agccatggcg gtgcgctact tctgccagac
    24541 gcggatgaag tcgatctcga ggcgtgccgg gaagtcttcg atgcgcaaag gcggcccggg
    24601 ccattcgccg ccgacggcca ggttgaccag cacgtgtgcc ggtccggcat cggcgccacc
    24661 cttgtgcgtc cagtcgaacg ggcgatcgac gacgagcttc tcgtcgacga agtggcgcac
    24721 gcgacccggc tcccacagga cggcaaacgt atggaagccg tccgcgtagt cgaatcccgg
    24781 gcggtaggcg ccgaacttgt cgagcagcgt ggcctgcgac ggtcggccct tgccctcacc
    24841 gaggtgcagg aaatgaaagc tgttcttcgt ggtgtcgcgg ccgttgtcga cgatctcgaa
    24901 gatgtcgatc tcgggcggcc agatctgatc ctgcgggttg atccagaacg ccggccacag
    24961 gccgcggcct gtcggaacct tcatgcggcc ttcgatgtag ccatactgac cgctccatcg
    25021 ggagcgcagc atgccgcttt cgatctgccc gttcttcagt tccttcgaaa cccgggccac
    25081 cagtgcgaag cgcccgttcc tgaagtcgtg gttgtcgttg tcgcggtagc gctgccactc
    25141 tcccttgagg gtgtcgagcg tgccgttctc gtagatgaag cgggtgtgga agttgtcgcg
    25201 caactcgtcg gcgttgctga cagtgcggcc gaacgtccag tccttgacga ggcggtagcc
    25261 ctgactggcg atcggagccg gtgccgcggg atcgaggctg cgggcggggg atacgcccgg
    25321 ccagccggca gcaatcatca gggccgcgca ggcctgcaac catcggggaa gatgccctgt
    25381 catgaccggg cctcgacaag cgcggcgccg ctgtgctggg gcagcagggc gtcgtagacg
    25441 ttgcgtgtgg cgtggccgat cccgatccat tgatcgctcg cgggcatggc gtcacccgtg
    25501 agccgctggc gcacggcttg ctccatcgct gcagccagcg ccgccgaatc tccaggcggc
    25561 acgagcacgc cttgtcggcc ctcgacgagg tcggctgcaa agatgccgac gcggctcgcg
    25621 atcatccagc ggcccagcga ttgagtgagg aaccagacgc cgcttgcatc gatctgccgg
    25681 tacggaaaca cgaagctgtc ggcttcgacg aagagcgccg ccatggcctc ctcgctctgg
    25741 cgctgcggtc gcaactcgat cgtgtctgcg aggccgaggg acgcgatgcg ttcttcgagc
    25801 ggggcgaggt tcatgcgcgg gcggcctgcg atgaccacac gcgtctggcg gcggatttca
    25861 tcgggcaggc gatggacggc ctcgatcaga acgtcgacac ccttgtaggg cttgatctcg
    25921 ccgaacagca caaagctgta gcgcccgtcc ggtcgctcgg tgcgttgcgg caacggcacc
    25981 tggagttgca gcgcaccgtg ggggatggtg gcgattttcc cgggcggtat gccgcgagcg
    26041 atcaaggtct ggcggccgct gtcggtgtga acgatcacgc ggtgcgccag tcgcatcggc
    26101 aggtcgaaac cgaggttctg cagcagcgac agacgttcgc cgttgaacgg cacggtgtcg
    26161 tgcacggtga gcacgagcgg gcaccagcga cgaatcaacg ccatcgccag tgcatcgagc
    26221 ggcggcacca ccgtccattg gaagtgcacg acgtcgggtg ggttcgctcg gatcttccgc
    26281 agcagcgcga ccaggccggc gaggtgtgcc gcacccttgg cgagcggctt gagcctggca
    26341 ggcagccagg tcgcttcgtc gacgcgcttg tagaagaacg ggtcggtgcg ctcgatgggc
    26401 agctcctgcc gatccccttt gcgtgtcggc cgagtgaccc agatcgggct gactcccgcc
    26461 tcaatcaggc ctccggtcag cgcggcatcg tatggcgcgg tgaacagcga cggatccacc
    26521 agaagcacgc ggagtgcgcg tcgatcggtc atggcgtcac gcgcccgctg gggctcatat
    26581 cgtgcgcggc agggtgttca ggctgccagg ccgattcccg cacgaaggcg agtcctgaaa
    26641 agaacccggc ggcccagcac acatgcatgg tgcctgccgc gaatccactg aggagaccgc
    26701 acatggaccg ctgctgccac gcgatcgaaa tggaagtgaa gcccagtgcc gcgacgtagg
    26761 ccagcggcca ggccaaagtc agcggataaa ccggcgccag ggatatcgcc agcgtcgaga
    26821 tgaccagatt ggccggcagc gccaactggc gtgcgcgcat cgaaccagga tggcgccgca
    26881 ccgtgcgtga gcgccctttg ccatacgaaa aatactgcct tgccagacct cgtaccgtgt
    26941 cacgtgggtg gtaactcagg cggatcgtgg cgtccagata cacctggctt ccgagcctgc
    27001 gctgcctgca atcgagttcg gcatcctcgt tgtgggtaaa gctttcgtcg tagccgcccg
    27061 cgcgcatgaa agaggacatt cgaaacgcgg cgtggtggcc gtgatcgacg aagccgctgc
    27121 gatgcccacc gcgatgcgcc gatccgccgg tgcccgccca gctgttggat acccaggcca
    27181 cggcgcgctg gaagcaggtc gttccgcagg agtccatcgg cacgaccacg gcgtcggcct
    27241 gcgtgcgatc gagcgtctcg agcaaccgaa gaacgaagcc ggccggatag gccgcatgcg
    27301 catcacatcg aacgagcaca tcgacgcccc ggccgaaagt cttcgcggcc agattgatcg
    27361 cagcgctctg gatgcgccgc gggttgatca gcaggtgcag tcccgggtcg gattcgctga
    27421 tttgccggac cagttccacg gtcccatcgg tgcttccacc gtcggccacc accagggtca
    27481 ctcgggcatc ggcgggaagg tcctgtctca gagaccgcag caccccgggc agggcgcggg
    27541 cttcgttcag tgtgggaatg accagcagaa cggagcgagc cgcttcaatg gtcattgatg
    27601 accactcccg caaacttcgc cggcccgcgc gtcacttcac tgaccaggcc ctgcaatgcc
    27661 ttcatccgac tcttgcctcg gcgtccgatg atcatggcca cgccgcattt ggctgccagc
    27721 actcggcaat ccgcaccgtg cgtgctcgat ggtgtgtcga cgatgacgtg atcgaacttc
    27781 cccagcaact cgttcacgag cagcccgaat gcgggacgct gcaacagttc gagcggattc
    27841 ggcggcagcg tccccaccgg caagacgaac aggctcggca ggtcctggag tgagtggatg
    27901 gctttcgtgt cggaacgacc ggaaaggatg ctgctcagac cgtagtcgtt gggaaccccg
    27961 aacaaggcat gctggcgtgg ggtgcgcatg tccgcatcga tcagcagcgt tcgtgcaccc
    28021 agttgactga agaccacagc cagattggcc gcaaagaagc ttttgccgtc gccgatgttg
    28081 gggctcatca cggcgagggc tcgcctcggc tgctcgggtg ccagggcacc gagcatcaac
    28141 tggcttcgga tgtcccgaaa ggactcggtc tggcggtgaa acggatcgac tgcggcgatc
    28201 agctcggagt tgaagctggc accatcgccc ggcgcatagg ggtagtggaa ctgctgcgag
    28261 agcgcccaca gcacgtcgtc ccgagtggcg agctggagtg caaccgctgc ttcgccaaat
    28321 cggatgccgt gcaggcgctg atgacccagt atctgttcga cctgtgcctc gctgaggcca
    28381 cgaacctgac ggatgaagtc cccgatcgaa cgatccagaa cgcgttcgtc cgccgctgcg
    28441 gcaggctcct gcggcggtac tgcagtgcgc gctttcagtc cgatcgggtg tgcaacgctc
    28501 atggagtgtc agtgggcgat cagcatggcg cgtccgagca cccgttctgc gacggaacgc
    28561 agcgaccggg cttcacgcgg aagacgaccg ctgcgcctgt cgggtatcac gccgagcaag
    28621 tgcagtttca tcgcatcggt gacgtccgat tccgtgcgaa gacgccagtc gcggacctct
    28681 cgcagcgcgg cagtcgtgat gcccaggatg gcgccgagca ggagtccgac cgtcaggttg
    28741 agcatcaccc gcggcgatga cgggaaaggc ggctcggtcg cgattttcac caccgaaaca
    28801 ttggtctgag tggcctggct ttcgagcgtg ctttgactga gcttgccgaa gcccgcgtcg
    28861 tagacccgct gggcgttctc gacatcgcgt tgcagtacgg cagcctcgtc gcgaatgccc
    28921 ttgagctgca ggaccttgtt tctctgcgcg tccagagcgt tgcgaaggtt ctcgagtctc
    28981 gactgattga ccgagttgtt cgcggtcagg ctgcccgtga tgcgactttt ctcgagagcc
    29041 agccggtcgc gcatctgccg gatgctcgcc ctcagttcca gcacctgcgg gtgctgggca
    29101 ccgtagcgtt cgctgatctc cgtgaggcgt gcctcctgcc tcgaaaggtc cgacgtcagg
    29161 gaaacgacca tggggttgtt gagcacttcc tgcatctggt ccgccatgcg agccgcctgg
    29221 ttctggcggc tgctggagtc gttggcaacg gcctgcagtt cgacgagctg cgtcgatagt
    29281 tctgccagtc gcgcgttttc tatgtcgagc cgttcgtcgg tggcgacgat gcctttcttc
    29341 tgctgaaact gggacagctt ggcctgtgcc acttcgagcg attcacgcag ctcctttgtg
    29401 ctctcgtcga acaaggcatt gaactgcttt gccggctcgg tgcgcaactc gagtgaagtt
    29461 tcgatgtagg ccgcgactac cgcgttggcg agattggcag cgaacttggg atcgcggtcc
    29521 gtgcaggaca cctgaatgac gttcgagtcc ttgcccggtc tcgcttcaag ctttcggccc
    29581 agcccttcgg ccagccaggc gttgaaatcg cctctgcctt ccgtctgttc cagccacgct
    29641 gctcgaaggc cggccttctc gtcgatgcgc agggccttga ctgcgcgaag catcacgcgc
    29701 tcgctttgca gaacgtcgac ctgggttgcc atgtagctgg acacgttcat tcccggcagc
    29761 acgatgccgg cgatcgggtc cggggacttg acatccagca ccacggcggc cgtggccacg
    29821 aacgtcttgc tgcgtgttgc ggtgaaggcc gcaacgatgg cgaccgtcat cgcaaacacg
    29881 aagagcgccg aacgccatcg ggcccggagg atcaacagca actgagagag attcatgcga
    29941 tgtcagtccg cggtagattg gcctgcattg cctaggatta acctgtattg caatctgcag
    30001 ttgcattgca tcggtcctta ttcagtctag ggcacgatca cgcacaatca agtgggaaag
    30061 cgggtcatta ccgttcatta caactgggcg cagattgatt caggttccag ttactgattg
    30121 attcgagatc attcggtcat gcgggcccgg gcttcgcgaa gtagccatct ggcgggtgtc
    30181 gtgggcgccg atgagacgcc tcgcgaacag gtgcaaactg gcgctggatc ccgtggcttc
    30241 acgacacagc cctgagagcg ctcgatgagc aaagccttca cccgagaatc agacgacagc
    30301 gaggacgacg aggagtccgg cgcgccggcc atgccgatcg gcgtgaagaa ctacgtcacg
    30361 ccggcgggct acgcgcgcct gcgcgccgaa ttgctgaacc tgctcgacgt cgaacgtccc
    30421 aaggtggtcg acatcgtttc atgggccgcc aagaatggcg atcgatccga gaacggcgac
    30481 tacctctacg gcaagaagcg actgcgcgaa atcgatcgcc gaatccgctt cctgaccaag
    30541 cggctggata ttgcagaggt ggtcgatccc tccgtgcacc atggcgccga tcaggttttt
    30601 ttcggagcca cggtgcggta catcaatgct cgcggtgaag cgcgaaccgt gacgatcaag
    30661 ggagtcgacg aggctgacag cagcgctgcc gagatcagtt ggatcgcacc gatggcgcga
    30721 gcgctgctcg gggcccgtgt tggcgacgag gtgaaggtgc agacgcccgc tggggctgac
    30781 ctggtcgagg tgctttcggt tcagtacccg ggcgcttgcc ccggctgacc agagcgccgt
    30841 tgaatcacgg cttgatgcga ctgacgcgca gcaccgcagc cgaacgcttc agcgtgcgaa
    30901 tcacatcggc cagatgcagt cggtcgcgca cgaccagcag aagcttcaat tcagcgttct
    30961 cggaagcggc ctgatcgccc atgtcgatgt gggtgatgtc ggcctcagcc gctgcaaccg
    31021 aggcagccac ctcggccagt acacccttgc cgttcttgag cagcaacgag acgccggtct
    31081 cgaacgatct tgtcggttgc tccgcccagt ccactttcag ccagccttcg ctgtcgcgac
    31141 cgagcaatcg ctttccgacc gaacattcgg ccgtgtggac caacaggcct tcgccgcggc
    31201 ccaggtaacc caggatcgag tcgcccggaa tcgggtggca gcaggcagcc agctggaccg
    31261 atgcaccttc gctgccatcg atgagcacca ttccctgcga gggagcctcg tccgcgccgt
    31321 agcgccccat ggtcagcgtc accgcatcgg ggcgcacacc gcgctcggtc agcagccgtg
    31381 acaggcgctt ggcgacgatc gaggcaatct tgcgccccac ggcgatgtcg gtcagaagct
    31441 catcgcggct gcgattgccg ctccagcgca gcagtggttg ccagaccggc gcgagggctc
    31501 cgctggcatc gtccagtggc atttccatgc cttcggcgcg cagggcctgt gccagcatct
    31561 tcagaccgag gtcctgcgac tcctcctgct ccatggtctt caggtggtgc ctgatcttcg
    31621 atcttgcacg gcccgtgcgc acgaagttca accaacccgg gttcggtcgg gcgccggggg
    31681 caatgacgat gtcgacgacg tcgccgctgc gcagttcggt gcgcagcgcg accggttcgc
    31741 cgttgacctt cgctgcaacg cagtgatcac cgacgtcgga gtggatggcg tatgcgaaat
    31801 caaccggcgt ggcgccgcgg ggcagcgcca ggatcttcga cttgggcgtg aagacgtaga
    31861 ccgcgtcggg aaagagatcg atcttcacat gctcgaggaa ctcggtcgca tcgcgcgtct
    31921 cgtcctggat gtcgatcagt gattgcagcc acatcgcccc gagccgctgt gcctcctggg
    31981 cattcgttgc cccgctgttc ttggtcttgt acatccagtg cgccgcgatg cctttctcgg
    32041 ccacggcatg catgggctcc gtgcggatct ggaactcgac cgcggtgcca agcggactca
    32101 ccagcgtggt gtgcagcgac tggtagccgt tcgccttcgg gatggcgatg tagtccttga
    32161 atcgccccgg caatggcttg tagagctggt gcagaacgcc cagagcgagg tagcactccg
    32221 gcagggtcga cacgacgatc cggaaaccga agatgtcgct gacctgggca aaggtcaggt
    32281 gtttctctcg catcttccga tagatggaaa acaaggtctt ctcgcggccg aagacctgca
    32341 ccatcagctt ggcctgggca aacgacttct cgacatcgcc ctgaatgcgc tcgaccacgt
    32401 cgcggcggtt gcctcgcgcc ttcagcagcg ccttcgacaa cgtttcgtgc cgccaggggc
    32461 gcagatactg gaaagccagt tcctgaagct gacgataggt ctgattcagt ccaagtcgat
    32521 gcgcgatggg cgcatagatg tccagcgttt cactggcgat gcggacgcgc ttgtccgcgc
    32581 tcatcgactc catcgtgcgc atgttgtgga gacggtccgc cagcttgatc aggatcacgc
    32641 gtacgtcgcg ggccatcgcc agaagcatct tgcggaagga ttcggcctgc gactcctcgc
    32701 gcgtcgagaa ctgcagcttg tcgagcttgg tcaaaccgtc gaccagctct gccgtcggtg
    32761 cgccgaagcg ctcgatcagc tctgccttgg tgatgccgca gtcttccatc gcgtcgtgca
    32821 tcaacgcagc catgatcgcc tgggcatcca gcttccagtc ggcgcacaga ccggcgacgg
    32881 cgatcgggtg cgtgatgtag ggctcgccgc tggcgcggta ctggccgagg tgcgcttcat
    32941 cggcgaagcg atacgcgtcg cgcacccggc gcacgtctgc cggatcgaga tagtcgagct
    33001 tgatggtcag cgcatcgaac gacgccgcag ctgcatcgga ggccacggat gcaaccggag
    33061 gtcgacgcca tgaggatctg aaggtcagag cctctttgac ggtttcaatg gtcgggatgc
    33121 ccatcacgcg aatttatcgc ttattgaagg catctgacac gcagctgtca ttgaccaccg
    33181 cagcgtgcaa cgaaaaacgg ccccgcaggg ccgttcgaga aggagtcagg gccagtcaga
    33241 ccgggacctt gcgcagcatt tcaattccga cctcaccggc ggcgatctcg cgcagtgcag
    33301 tcaccccggg cttgttcttg gtctcgatct tcggggcgtg cccctggctc agcatgcggg
    33361 cgcggtaggt ggcagcgagc accaactgga accggttggg aatatgaacg aggcagtctt
    33421 caacggtgat gcgggccatg cggcgctcct ggggtgtgac ggtgtcggtt ccggcatgga
    33481 ttcagtcgag gagatccagc gcccggaaaa ctgccggttt gttcttcgca agcaccgcat
    33541 accgcaggcg ttgcgaatgg acgatggtct tgaggtcgaa aacggcggtc tcgaacaagg
    33601 agttgatgat aacaaagtcg aagttgcgag cctgtgcaac ctcgagcctc gcgttcacga
    33661 gacgttgtgc gatcacatcc gggtggtcct cgccgcgccg gttcaggcgc tgtgcaagtt
    33721 cctcataact cggaggcagg atgaaaacga gtaccgcact cggaaacagg cgcttgatct
    33781 gcagggcccc ttgccagtcg atctccagca ccacatcgtg gccctgcgag atgcggcttt
    33841 cgatggccgt tcgcgacgta ccgtagagat tgccgtggac ttctgcccat tcaaggaact
    33901 cccccgccgc gatcttcgac cggaaaactg attcgccgac gaaatggtac tcgcggccat
    33961 cctgctcctg gccccgcgga gctcgagtgg tgtgagatac cgagacgccc agatgcgaat
    34021 cgagttcaag caaggccttg accaggctgg acttgccggc gccgctgggc gccgcgacca
    34081 cgaaaagact gcctgcttgc tgcatcggcg tttcactcct ggttgtcagc ccgaaccctg
    34141 ccagctcgct acagggcacg catgtgctgg atttgccacg attgttgacg catcttgcct
    34201 gcctgacccg cgctgtgcga cgagagagcc gtgtacgctt ccagttgtcg gtcctgcacc
    34261 gcggcaaagt agccgtttcg atggtgcgag ccgctcacta ctttgatcac caccatgaca
    34321 tcggattcgc gcgccttgct gttgattgtc gaggatgacc ccgcgcttca gaagcagatc
    34381 aagtggtcgc tggatcgatt cgagtcggtt acggccgacg atcgggcgac cgcgctggca
    34441 cagtttcggc ggcacacccc tgccgtcgtg acgatggatc tggggctgcc gccgcatccc
    34501 gactccgtgg acgagggctt caagctgctt gcgcagatga tcgagctgaa tccggacgtg
    34561 aaagtcatcg tcctgacagg gcagaacgat caatccaatg ccttgcgggc gatcgacctg
    34621 ggcgcctacg acttcctggc caagcccttt gatcctgacg tgttgggctt gatcatcagc
    34681 cgggccttca atctcttcga gctgcaaagc gagaaccggc gcctgcgcga gatgcaacag
    34741 cccgacctgc agggcgggct gatcacgcgg gatcagcaac tgttgcgtat ttgtcgcact
    34801 atcgagaagg tggcaccgag caacgccacg gtgttgctcc tcggcgaaag cggtaccggc
    34861 aaggaggtgc tcgcgcagtc actgcatcac gcttccagac gagccggcaa gttcgttgcg
    34921 atcaactgcg cagcgatacc cgaaaacctt cttgagagcg aattgttcgg ttacgagaag
    34981 ggcgcgttca ctggagcggc taagaccacg cccgggcgca tcgagacggc tcacggtggc
    35041 acgctgatgc tcgacgaaat cggcgatctg ccgatggcat tgcaggccaa gttgcttcgc
    35101 ttcctgcagg aacgcaccat cgaacgcatc ggcggtcgcc aggagatccc agtcgacgtc
    35161 agggtggttt gtgccactca tcaggacttg ggcgagttta tcaagacaag ccgcttccgg
    35221 caggatctct actaccggtt ggccgagatc gtggtcgaca ttcctcccct gcgtgcacgt
    35281 cagggagatg cggtgttgct ggcccaggcc ttcttgcggc ggtttgcgcg cgaacagaat
    35341 cgcagcgcca tgggctacac agacgacgcg ctggctgcga tagaagcgta cgggtggccc
    35401 ggcaatgttc gcgaattgct caatgccatc aagcgggtct cgatcatggc cgagggcaat
    35461 cgggtcacct cagaagatct cggactgccg aatgttgcag gggctgattc gggtgaagac
    35521 gcagggctgg atctgcgcgc ggtgcgcgac aaggccgagc ggggagccat cgtcgcggcc
    35581 ttggcacgat cggacggcaa tctcgtgaaa gcctccgaaa tactgggtgt cacgcgcccg
    35641 acgctttacg acctgatgcg tcgtctgacg atccaatgga agggcgtcga gcccccttcc
    35701 gccaagtagg tatcgccagt caacgtgctg cagccaacat tcgcagcacg aattcgaccg
    35761 gaatcgcgta gctgatgccc gaaggatggc tcaaggcgga ctcgcgagtg cccttgaggg
    35821 ccaccatgtt gacaaccccg atgacggcac cggtttccag gtcaaacagt ggcccgccac
    35881 tgttgcccgg gtaggcagtg gcgtcgagct ggatgaaatc gaatgagcct tccttgatcc
    35941 ggctgatggc ccgtgcatcc agttgtctcg ctgtcgggct
//
LOCUS       DAOORV010000018        35166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1416_length_35166_cov_7.044032, whole genome shotgun sequence.
ACCESSION   DAOORV010000018 DAOORV010000000
VERSION     DAOORV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 35166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35166
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_35166_cov_7.044032"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="PKV56_04250"
     CDS             <1..1896
                     /locus_tag="PKV56_04250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOX67054.1"
                     /translation="EGTVDFIPNYDGSTQEPRQLPSRLPFVLLNGASGIAVGLATEIP
                     SHNLREVALAAIALIRNDRLDDDELAALIPGPDYPGGGQIISSADEIRAAYATGRGSL
                     KVRARWKIEDLARGQWQLVVTELPPGTSSQRVLEEIEELTNPKVKAGKKALSADQLQL
                     KASVLGVLDAVRDESSKDAPVRLVFEPKTSRIEQSELITQLLAHTSLESSAPINLTMV
                     GADGRPTQKTLRQMLVEWIAFRLQTVQRRSQHRLDKVLDRIHVLEGRQLVLLNIDEVI
                     RIIRGADEPKPALIEHFKLSERQADDILEIRLRQLARLEAIKIEQELKELRVEQGKLE
                     DILGNPGTLKRTVIKEIEADIKQFGAVEKDPRRTLIQVEKKAVVEIKVIDEPVTVVVS
                     AKGWVRALKGHEVDPAGLQFKAGDALLATFACRSVDPLLIFGSAANGSGRVYSTAVSL
                     LPGGRGDGQPITTLIELEAGTQPAHYFAGHAAQTLLLAGNAGYGLLARVGDLTARQRS
                     GKAFLTLEAGEQLLPPALASAGMLGDQVGCLSSQGRLLVFPLSELKVQPNGGRGLTLM
                     DLEPRDTLASVAVFAQALRIEGTGRGGKARDEVLKGASLAPYLGKRARKGKSQDAIPK
                     AQRLLPA"
     gene            2066..2971
                     /locus_tag="PKV56_04255"
     CDS             2066..2971
                     /locus_tag="PKV56_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HOX67055.1"
                     /translation="MHPSIVQRADSSNAFARVPPEPRFVPSQLSYLRPMSECPVSYAF
                     EPPPGTPWESGEYEEQTVQITDARSAPQAPCIDHEGYVLRDAPTAVTNFLDEDAIRTT
                     YYREAAELALAVTGARHAHVFDHLVRRREAGRAPLSFGRRDAGGRAAANGRVHNDYSD
                     ASGIRRLAMVLPQCDVARVRRYGIVNLWRSINGPVQDTPLALCDARTLSAFDLVTSEV
                     RYPRRTGEIYLARFSARHRWAYFSEMDRHEALVFKQFDSQLGGVARFTPHAAFDLPDV
                     PPGTPLRESIEVRCLVVYEQEDGAS"
     gene            2968..3600
                     /locus_tag="PKV56_04260"
     CDS             2968..3600
                     /locus_tag="PKV56_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOX67056.1"
                     /translation="MSEVKTGKAPADLEFWFEFGSNYSYLSVMRIEAAAARLQVPIRW
                     KPFLLGPIFRTLGWDTSPFVLQKAKGEYVWKDMERACRDLGLPWIRPTTFPRTALLPM
                     RVALVGAEQPWMGAYCRRVMSLNFAEDRDIDAPDVVADVLKQLGLPARQIIDEARSEP
                     NKLKLREQTEAARARGVFGAPTFLVGDEMYWGNDRLDDALACASLRLKPE"
     gene            complement(3587..4504)
                     /locus_tag="PKV56_04265"
     CDS             complement(3587..4504)
                     /locus_tag="PKV56_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOX67057.1"
                     /translation="MSLPLVRLSSLDLIRGYVAVGRRMSITLAAQDLCLTQSAVSRQI
                     RALEDRLGVKLLVRGHRSIAFTPEGERLFRSADGAVQQLQDVLGDISTRGSARPVTLS
                     ASIGVTGLWLLPRLSHFQAAHPGVDLRVSANNQVADLRHDGIDLAIRYSASAPEGATW
                     LFGESLMPVAHPGLGLRSLGSAKALSRISLLEFDDPRHPWLQWEDWLAAKGWADTRPR
                     GVLHFNQYDQVIQAALAGQGVALGRLSLIQPLLDAHRLVALAPHDKQPETTHAYWLIR
                     GEREPRVAVRQVAAWIEAEAASSRAVTPA"
     gene            4642..4923
                     /locus_tag="PKV56_04270"
     CDS             4642..4923
                     /locus_tag="PKV56_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOX67058.1"
                     /translation="MDYVLITHAVKDYEAWKQVFDDAAPMRKSAGEQRYFVLRHEDDA
                     NRIVHFSQWASIDRARSFFESPELVEIRRLAGVESPEFSYLRSLEEGVL"
     gene            4920..5405
                     /locus_tag="PKV56_04275"
     CDS             4920..5405
                     /locus_tag="PKV56_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX67059.1"
                     /translation="MTTPRQPPVFARADQVPPRVKASSYPEPFASRMQGRTKRPLGEL
                     FGLNNFGVNLTTLNPGAVSALRHQHLRQDEFVYILQGEATLVTNEGETLLLPGMCAGF
                     AAGNGDAHMLINRSGLSCVYLEIGDRTPGDHATYPDDDLVAFSTQQGAWQFTHKDGQP
                     Y"
     gene            complement(5452..5955)
                     /locus_tag="PKV56_04280"
     CDS             complement(5452..5955)
                     /locus_tag="PKV56_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOX67060.1"
                     /translation="MASSSSTTGTTRLLLAMALACVGSVAGALVSQHVFDMQPCPWCV
                     LQRVIYLAIAMLCLLGAAWSSIVARRGIGVLVLLLALSGAASALYQDLVASKSASCNL
                     TFADKVISALGLDGAFPPLLQVTANCADAAVKLLGIQYAYWSLALFVMLAAASILVVA
                     SKPRRRY"
     gene            complement(5958..6425)
                     /locus_tag="PKV56_04285"
     CDS             complement(5958..6425)
                     /locus_tag="PKV56_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOX67061.1"
                     /translation="MTASSSVYDRLKELGIELPPLAIPAAAYLPFVRTGNLVFLSGHI
                     AKRDGKPWVGQLGLNMDTATGKLAARAVAIDLLGTLNAAIGNLDQVERIVKVMSLVNS
                     TGTFTEQHLVTNGCSELLGDVFGTKGQHARSAFGVAQIPLGACVEIELIVQVA"
     gene            6480..8969
                     /locus_tag="PKV56_04290"
     CDS             6480..8969
                     /locus_tag="PKV56_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOX67062.1"
                     /translation="MPAPRATDAANTGWRLAMLGAAWPAGAAVQLREATLQAMPVYVG
                     CMVLGLLLVAAATLAGRRSMAAALGLMVLAFGVTGGRASIRLAETLPITLEARDLQVT
                     GWVSSLPQRSSNGLRFRFEVEQATLDGQPITVPGVVSLGWYAGFQDGAGLTAGQLRLR
                     AGQLWQFTVRLRRPHGLSNPHGFDQELRWFEDGVRATGSVRDATAPRLLDGAVAHPIE
                     RLRQHVRDAVEASVVDRRAAGVLAALAVGDQSSIERDDWDLFRRTGISHLVSISGLHI
                     TMFAWLSAAAIGALWRRSERAMLWLAAPSAGMWGGFAVAGLYAVFSGWGVPSQRTVWM
                     LGTACLLRSAGLRWPWPLVLLAAATAVAVIDPWALLQPGFWLSFAAVGLLMLSGRAQF
                     EASGAVPAASADPLATAGTRGRALGPALRGWVLGGVRTQWIATVALTPLTLVCFQQMS
                     LIGFVANLIAIPLITLVVTPLALLGCIAAPLWTVGAWFVSLLCELLGWMAQAPASVWS
                     VAVAPWWAQAAGLFAAAWMVTPLPWRVRALAVPLLLPLLLPARDLPAVGQFELVAADV
                     GQGTAVIVRTRLHVLLYDAGPQYSPEFDAGQRVLLPLLRARGDHRIDMLVLSHRDTDH
                     VGGAATLLQGLPIEELSSSIEDLHPLRIDVRARGLKDGRCTGGQHWEWDGVRFEVLHP
                     ANDADTGGLRPNTVSCVIRVQGAGASALLTGDIERDQEIRLLRERRESLASDVLLVPH
                     HGSRTSSSPAFLEAVDPRVAVFQAGHLNRYGHPAADVLARYRERAIFAVDSPNCGAWT
                     WYSASRNDNPMAGCQRVLARRYWQWRASDAP"
     gene            9122..10558
                     /locus_tag="PKV56_04295"
     CDS             9122..10558
                     /locus_tag="PKV56_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOX67063.1"
                     /translation="MRSSFDEMQISSTQVREHYRGYEQWLAQQPGDVMKSRREEAEMI
                     FRRVGITFAVYGAKDEDGSGTERLIPFDLIPRVIPAHEWVDMEKGLRQRVVALNRFIH
                     DVYHGQDIIRAGIVPGDQIFKNAQFRPEMMGVDVPFGVYSHISGIDIVRAGNADGSGS
                     YYVLEDNLRVPSGVSYMLENRKMMMRLFPELFSQHRVEPVAHYPDMLLETLRAVAPAS
                     VNEPTVVVLTPGMYNSAYFEHAFLAQQMGIELVEGQDLFVKDNFVYMRTTQGPKRVDV
                     IYRRVDDDFLDPTAFRPDSTLGCAGLLGAYRAGNVTLSNAIGTGVADDKSIYPYVPKM
                     IEFYLGEKPILNNVPTYLCREKDDLKYVLDHLAELVVKEVHGAGGYGMLVGPASTKAE
                     IAEFRAVLEANPSGYIAQPTLALSTCPTFVESGIAPRHIDLRPFVLSGKTVQMVPGGL
                     TRVALKEGSLVVNSSQGGGTKDTWVLED"
     gene            10561..11604
                     /locus_tag="PKV56_04300"
     CDS             10561..11604
                     /locus_tag="PKV56_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOX67064.1"
                     /translation="MLSRTADHLFWMARYMERAENTARMLDVNYQTSLLPQSADAAEQ
                     GWKGLLSISELSGDFAARYGKVTARSVMEYMVSDPENGSSIRNCLMAARENARAVRGT
                     LTTEVWETQNQTWLEFQRMVANGAFLKDPGTAFEWVKFRSHLSRGVTVGTMLQDEAFH
                     FLRIGSFLERADNTARLLDVKFHAVESEFFGGPHSMGSTAPSAPGEAESSGSSQSQGS
                     PARSGRDVEYDFYHWSAILRSVSGFEVYRKVYRNVIRPEKVAELLILRPDMPRSLAAC
                     TQEVLSNLTMIANEHSSETLRRAGRLRADLQYGRIDEILATGLHAYLTQFLERVGTLG
                     VGISRDFLVPVES"
     gene            11607..15020
                     /locus_tag="PKV56_04305"
     CDS             11607..15020
                     /locus_tag="PKV56_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOX67065.1"
                     /translation="MSIHVALNHVTHYRYDRPINLGPQIVRLRPAPHSRTRILSYSMR
                     VEPATHFINWQQDPQSNYLARLVFPDKTTSFRIEVDLVAEMSVLNPFDFFLEPSAENF
                     PFQYEPELAAELAPYMAKGELTPLLKKYVDSVPRTKKHTNDFLVELNQKLQKDISYLI
                     RMEPGVQTPEVTLTNASGSCRDTGWLLVQILRHLGLAARFVSGYLIQLKSDVKSLDGP
                     SGTEVDFTDLHAWCEVFLPGAGWIGLDPTSGLLAGEGHIPLACSPDPSSAAPVSGAID
                     ECETEFEHHMSVTRVWEAPRVTLPYTDAQWESIEALGHRVDEDLRRNDVRLTQGGEPT
                     FVSVDDRDGAEWNTEAMGPTKRLLSADLMDKLRAKYGDGGLLHYGQGKWYPGEQLPRW
                     SLNLYWRKDREPVWTHPELFASEHKDYQVTDVHAQKFLSGVARRLALDPKYVFPAYED
                     TWYYLWRERKLPTNVDPFDARLSDPLERERLCKVFSQGLDKVVGHVLPVARGTHTQAR
                     WQTGPWFLRTERCYLVPGDSPLGFRLPLDSQPWAAEGDMPWVYPPDQTQQFVALPPYR
                     RLRYANGSAEGAGSPAGPGSAAGTGAGFAERSRAAQGPEKAVASAPAPSLRVPSSFES
                     AGWISRTALCAEPRGGRLYIFMPPMSVLEDYLELVSAIEDTAAELKVPVILEGYEPPK
                     DPRLSTLRVTPDPGVIEVNIHPAHCWEELVDQTTHLYQSAHETRLSTEKFMLDGRHTG
                     TGGGNHFVLGGATVADSPFLRRPDLLASMVAYWHNHPALSYLFSGLFVGPTSQAPRVD
                     EARNDSVYELEVAFAELQRVTRENGDYCPPWLIDRLLRNLLIDVTGNTHRAEFCIDKL
                     YSPDGPTGRLGLLEMRAFEMPPHARMSLTQQLLMRALVSRFWKEPYRPARLKRWSTEL
                     HDRFMLPHFIWQDLCDVVEELNAFGYALKADWFAPHLNFRFPRLGDFTAMGTEVELRL
                     ALEPWHVMGEENAAGGTARFVDSSVERIQLKATGLISDRHVLTCNGRVVPMQPTGQVG
                     EFVGAVRYRAWNPPSALHPTIDVHAPLTFDLLDTWMSRSMGGCQYHVAHPGGRNYDTF
                     PVNAYEAEARRLNRFFRTGHTPGSMQVPKDERNPNFPFTLDLRRAPSST"
     gene            15147..17642
                     /locus_tag="PKV56_04310"
     CDS             15147..17642
                     /locus_tag="PKV56_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOX67066.1"
                     /translation="MLSRLLSGYASVEGRYDELLSAPGIPRPHWDAFLRSLAARQGLE
                     VSETLALMESEIRENGITYNVYADPKGADRPWEVDPLPLLLSATEWDEIAAGIAQRAD
                     LLNRVLGDIYGPQELLRSGAIPPSIIFGHSGYLHQVQGIRPPGGVHLFNYAADLARSP
                     DGHWWVVNDRTQAPSGSGYALENRLVVSRVFPQMFRDLHVQHLASFFDALRESLLRWA
                     PKDMADRASERANPLVVLLTPGPYNETYFEHALLARYLGFPLVEGSDLTVRDGCVWMK
                     TVEGLKRVNAILRRQDDDYCDPLELRSDSALGVPGLTDCARRGTVLLANALGSGVLES
                     GGLLGYLPKLSEQLLGEPLRLPSVATWWLGEPAALDDAWSRLDKLIIKPLERSIREPA
                     VFGADLSAKERAALRERVAARPQRYVAQEWVHVSQAPVIEQGSDRQRAEQLGSRTVGL
                     RVFAVATPSGYHVMPGGLTRVAGDDQSRVIAMQRGGRSKDTWVLSDAPVNASFSLLSR
                     TVKPQDLVASRANVPSRAAENLFWFGRYGERCESAARLLRVAIGDVLDDSRESADGVA
                     PTLILAQRFGLIDNTDNPSTDLLRAATHPDEGLSERLRQLSRVAFNLRDRMSADHWRS
                     LNQLISDPVFQRGLSSTSLGLPLAMGWLDRAVTSMMTLSGFVLDGMTRGVGWRFLSIG
                     RRIERLSTLCNVLQVAINEGRSHGLDWLLEYGDSTVTYRSRYLVAPEWLPVLDLLLRD
                     EVNPRSIAFQVKGLAEYVAKLELSHGRFASDVLAPAHYALRNLDTLDLNPESEALADV
                     LVQLQRAAHAVSDELTLKFFSHAASRSVLSLVA"
     gene            17665..18594
                     /locus_tag="PKV56_04315"
     CDS             17665..18594
                     /locus_tag="PKV56_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOX67067.1"
                     /translation="MSQQQSQQQQHPSMVFDRYTVEHETRYAYTAPVSQSWQLGRLTP
                     RVLPWQRLVSHSLQIEPSPDERHSATDSFGNSITHFGLHGAHRQLTVRMQCQVEVGDR
                     PAVDPRESMTVDEARLAVLRRQNAAEDLGAARMAEPTLLVPLSEAARIYAASSLRPGR
                     PWVEALTDLTHRIHRDFEFDAGATTVSTSVDEVMQHRRGVCQDFAHLMLACLRGHGLS
                     ARYVSGYLLTDPPPGMPRLMGVDASHAWVAAYLPGQGWIEFDPTNDQLADRRYITLAW
                     GADFADVVPLRGVILGGGMMQGMDVSVSVIPMT"
     gene            18751..19011
                     /locus_tag="PKV56_04320"
     CDS             18751..19011
                     /locus_tag="PKV56_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67068.1"
                     /translation="MFSTGSTPGSSDLIARAIVFLSLCPAFPLPARSVETLVEAAESA
                     VRGPGWYDSSWELSCGLDIAEVPSLDDWIGVCQGDAPLSAHP"
     gene            complement(18995..20224)
                     /gene="mltB"
                     /locus_tag="PKV56_04325"
     CDS             complement(18995..20224)
                     /gene="mltB"
                     /locus_tag="PKV56_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HOX67069.1"
                     /translation="MYFLPDDTVRPMGHRPPEISTRPTRLWAHALSLALLLCAPTHSP
                     AADGTGTHAAKPGAAKKMHPAKPRSKVRSDADPDIVTYGRRADVMQFATEVAARQGLD
                     VEAVQATLAQSRFVPDVVKFIMPPPTGTAKNWAAYRARFIEPRRVRAGVEFWRVNETW
                     LARAEQRYGVPPEIVVGIVGVETLYGRHMGSFRVIDALATLSFDFPRERKDRSAFFRD
                     ELEQLLVLGQREGSDPLALKGSYAGALGMPQFMPSSVNKYALDFDGDGHIDLHGSAAD
                     VIGSVAHYLAEFGWQRGLPTHFEVTPPSDPGERALLLAPDIVPSFTAQVFVEHGAVFG
                     ARAPDLAASDAGTADDGKGAAHDVGLLALVELQNGDAAPSHVAGTANFYVITRYNWSS
                     YYALAVIDLGTAVKGAR"
     gene            complement(20232..22298)
                     /locus_tag="PKV56_04330"
     CDS             complement(20232..22298)
                     /locus_tag="PKV56_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOX67070.1"
                     /translation="MKTRSFAWPGWAHLPRDSRDTLFLLVVIGWTVLPHLSHLPTWCT
                     VLTGVVLLWRARLALVGAALPGRWVLLGVLVIATGLTFWSFRSLLGKEPGVTMAVALM
                     ALKTLELRARRDAFVVFFLGFFLILTQFLYSQSLLVAAAMLVSIWGLLTALVLAHMPA
                     GSPSLRQAAALSGKTALLGAPLMALLFLLFPRVGPLWGVPQDGLSSTGLSNVMSMGTV
                     AALAQDDSIALRIRFEGAPPAPQDVYFRGPVLGHFDGRDWTPLKPTFPPPLTPRADLR
                     VEGAPVRYEMTLEPLRLAVIPMLEASPSGPVVEGVRVAQHDDLEWVAERPLYERIRFT
                     GIAYPRFTHGPQQPVIGLQDYIDLPPGHNPRTLAWAAALRRDARYAQANGTTLAQVLL
                     SHIRTGGYGYTLTPGLYGEQDPRSAIDEFWLDRRLGFCEHYAAAFVVVMRALGVPARV
                     VTGYQGLDPTPVDGYRIVRQSYAHAWAEYWQADVGWVRADPTSAVAPDRVLRSRPLVT
                     PPGLMAGALNGVNPQLLAGLRDGWEALNNRWNQWVLSYSRGSQLDLLQRLGFSAPGWE
                     DLALLLVGAFSLLALAGAAWAWWERHHTDPWVRQLDRLRAALRRLGIGAADHDAPRTL
                     AKRVRTQLSEAGEPLAVMLDRIDVQRYGRECTSRPDGRLTREFLALARRLKPMAADVS
                     PTARNQ"
     gene            complement(22295..23281)
                     /locus_tag="PKV56_04335"
     CDS             complement(22295..23281)
                     /locus_tag="PKV56_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX67071.1"
                     /translation="MTPTARLRGRLRRRVDAWWQSRSPRSDSLLLTQRNVYILPTRAG
                     MMFCFTLGVLLIASINYQLNLGYLLTFLLAGSGVVSMHMTHNTLRGLTLHLRPVGPVF
                     AGDTARLEAVLSSPGSARYGVGLRLQDASASTLGYTNVPAGGQATLHLGYVPPQRGQH
                     ALPTLNAETRFPLGLFRAWAVWRPAMSQVVYPQPERPASALPATRLVAGGTVQSHASS
                     SGEFEGIRGYRRGDPLKGIAWKQAAKAMASGGDLVSRDSSTQSQQQLWFDWQSCGIGG
                     TEGRLSRLTAWIIEADRRDAAWGLSLPGCEIPIDQGEAHRRRALEALALWSP"
     gene            complement(23278..24216)
                     /locus_tag="PKV56_04340"
     CDS             complement(23278..24216)
                     /locus_tag="PKV56_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX67072.1"
                     /translation="MNAVPAHRLTEELARLIDQISTIIVGKREQIRDCVACLLAGGHL
                     LIEDVPGVGKTTLAHALSVSLGLRFSRMQFTADMMPTDLIGVSVYERSTQTFVFHPGP
                     VFAQVLLADEINRAGPRTQSALLEAMEEQQVTVEGETRPLPRPFFVIATQNPTDQLGT
                     YPLPESQLDRFLMCITLGYPDRASERELLAGQDRRDAVGRLRAVMSPERLIEAQQAVL
                     AVHASGALLDYLQALIAATRSGVWFTEGLSPRAGIAVLRAAKAHALLDQREHVAPDDI
                     QAILVQTTAHRLIPVPGAGRGRAEQVRAMIEATPLP"
     gene            24266..25231
                     /locus_tag="PKV56_04345"
     CDS             24266..25231
                     /locus_tag="PKV56_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOX67073.1"
                     /translation="MPTAFYSHPDCRGHDMGRGHPESPQRLDAIDDHLRAVGLDIALE
                     ARDAPLVDLADVALAHDSGYVTEIQHVLQQVMADGEPRSLDPDTIASPGTWNAVRRAA
                     GAAVAATDAVIDGNARNAFCAVRPPGHHATRHAAMGFCFFNNVAIAARHAVDVRGLER
                     VAIIDFDVHHGNGTEDIIAGDERVLMVSFFQHPFYPYSGGVPMGPNMVNVPIPAYTRG
                     PEIRSLIEANWMPRLESFRPQMLFISAGFDAHREDDLGQLGLVEADYAWITRRLMAVA
                     QRHSKGRIVSCLEGGYNLSALARSVAAHLRVLAGVDGVESSEPHP"
     gene            25228..26493
                     /locus_tag="PKV56_04350"
     CDS             25228..26493
                     /locus_tag="PKV56_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOX67074.1"
                     /translation="MTRPLDSAELQDLVDALFQPSALTELGALLLCLALAWLLVRLVR
                     GHPPIDAPIWFGRRIVDGVLFPLLALVLAFSAKLLLTGVVKAAVFKLAIPILMSLLVI
                     RLTVRVLSVNYPESRWMRIIERSISWIAWLAVVLWVTGVLPLLLEAMDEVRWMAGGTE
                     LSLRNVVEGALTIGLVMVLALWVAAAIERRLLSGSGNDLSIRKMVANIIQALLLFVGL
                     LFALSVVGIDLTALSVLGGAIGVGLGFGLQKIAANYVSGFVILAERSLRIGDMVKVDN
                     FDGRITDIRTRYTVIRALNGREAIVPNEMLITQRVENSSLADPRVLLTTTVQVAYGTD
                     VRALQPLIAQAVAGVARVLPDPTPTVQLVEFAADGMNILVNFWILDPENGQGNVRSEV
                     NLALLAVLDEQGIEIPYPQRVLRRHHTPT"
     gene            complement(26553..27320)
                     /locus_tag="PKV56_04355"
     CDS             complement(26553..27320)
                     /locus_tag="PKV56_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOX67075.1"
                     /translation="MESDPDTKAEQTRTLWAERDRHALESPSAEFGAAGTPGPQEAPG
                     RPTRPCLVDPRNVPSRSPSTIEGRAALLHSIAHIEFNAINLALDAVWRFDAMPARFYL
                     DWLQVANEEALHFSLLREHLRGLGFDYGDFDAHAGLWVMTQRTADDVLARMALVPRTL
                     EARGLDATPPLQAKFRRAGDARAVEILDVILRDEIGHVAIGNRGYRWLCTQRGLDPLT
                     HYDVLAERHQAPRLRPPFNHAARRAAGFTDEEVALLG"
     gene            27423..27965
                     /locus_tag="PKV56_04360"
     CDS             27423..27965
                     /locus_tag="PKV56_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOX67076.1"
                     /translation="MAVYRLGDAVPSIAPSAWVADSAQVIGDVVLSESASVWFGAVLR
                     ADNTRLLIGARSNVQDGAVIHSDTGWPATLGCDVTVGHQAMLHGCTIGDGSLIGIQAV
                     VLNGARIGKSCLVAAGAVVTEGKEFEDGWLIMGAPAKAVRPLTPEQLAGLARSASGYV
                     ANAQRFRSQLARIDTQAPHE"
     gene            27958..28926
                     /locus_tag="PKV56_04365"
     CDS             27958..28926
                     /locus_tag="PKV56_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOX67077.1"
                     /translation="MSELHKFIFEGMPVRGMLVRLTEGWREVLQRRVTSHDEFPEPVR
                     VLMGEMAAAGALMQAGIKFDGALVLQVFGDGPVKLAVVEVQSDLAFRVTAKVVGDVAA
                     DAQVEAMLNVHGKGRCAITLDSRTKFPGQQPYQGVVSLHGDQREPLQKISEVLEHYML
                     QSEQLDTRLILAANDDVAAGLLIQRLPVEGAGNLGRRNEDEIGVNEDFNRLSMLAATL
                     TRDELLTLDADTVLRRLFWEEPLKRFPPLAGEDGPRFACTCSRERVGRMLMGLGRTEV
                     DDIIVEQGRVEIGCDFCGIKYHFDAVDVGELFTPTPQQAPGSTTLN"
     gene            complement(28919..30307)
                     /locus_tag="PKV56_04370"
     CDS             complement(28919..30307)
                     /locus_tag="PKV56_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOX67078.1"
                     /translation="MHPLLKDAARRASQYLDRLDERSVAPLPGAVSRLKQIECDLAEA
                     PQRPQDVLRRLDELGSPATVASNGGRFFGYVVGGTLPVSMAAHWLATAWDQNAPYPAA
                     APGAAAFEHTALRWIIDLLGLPSTVAGAFVSGATTGNLCGLAAARHAVLAQAGWDVDA
                     QGLSGAPPITVVIGDEAHPSVTKVLGVLGLGRDRAIRVPVDAQGRMHADQLPRLSGPS
                     IVCLQAGNVNTGACDPFSSLIQKAHQDGAWVHVDGAFGLWAQATRQHRHLCDGVTEAD
                     SWATDAHKWLNVPYDSGLVFVRDREALRSTMGVAAPYLAADDDGAAASDLTPELSRRA
                     RGIDAWAALASLGRSGLREMIERCCRHAQRFARGLGDAGHEVLNDVVLNQVLVRFGDS
                     DTTRRTIDAIQRDGTCWCGITYWQGRTAMRISVCSWATTDDDVDRSLAAMLRCAQECG
                     APPKGSAGRALS"
     gene            complement(30384..30662)
                     /locus_tag="PKV56_04375"
     CDS             complement(30384..30662)
                     /locus_tag="PKV56_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOX67079.1"
                     /translation="MRFISFALLALIGLVHAELWFGKSGVPHVLDLRSRLEEQNGINE
                     TARQRNEQLAAEVNDLKEGLEMVEEKARLELGMVKPDEILVQWSAAAK"
     gene            complement(30712..31995)
                     /gene="eno"
                     /locus_tag="PKV56_04380"
     CDS             complement(30712..31995)
                     /gene="eno"
                     /locus_tag="PKV56_04380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOX67080.1"
                     /translation="MSAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREANELRDGDPKRYLGKGVMRAVEHVNTEISEAVLGLDASEQAFLDRTLIDLDGTDN
                     KSRLGANAILAVSMAVARAAAEESGLPLYRYFGGSGGMQLPVPMMNVINGGAHANNNL
                     DLQELMIIPVGAPTFREAVRYGAEVFHALKKIIHGKNMSTAVGDEGGFAPSVANHEAA
                     IQLILEAIDKAGYTAGEQIAIGLDCAASEFYKDGKYVLGSENLTLSAPEWTDILATWV
                     DKYPIISIEDGMAENDWDGWKHLTDRLGSKVQIVGDDLFVTNTAILKEGIEKRIANSI
                     LIKINQIGTLTETFAAIEMAKRANYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSM
                     SRSDRMAKYNQLLRIEEDLGDVASYPGRSAFYNLR"
     gene            complement(32064..32921)
                     /gene="kdsA"
                     /locus_tag="PKV56_04385"
     CDS             complement(32064..32921)
                     /gene="kdsA"
                     /locus_tag="PKV56_04385"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOX67081.1"
                     /translation="MKLCGYEVGLTQPFFLIAGPCVVESEQLQMDVAGALKEMTAKLG
                     IPFIFKSSYDKANRSSGNTFRGPGMERGLEILGKVKRELQVPVLTDVHAEDEIAAVAA
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPHDMRNVIDKARAAAREMGLP
                     EDNFMACERGVSFGYNNLVSDMRSLAVMRETGAPVVFDATHSVQLPGGQGTSSGGQRE
                     FVPVLARAAVAVGVAGLFMETHPDPMNAMSDGPNAVPLRHMRALLEQLVSLDRVVKQQ
                     PLLENDFSC"
     gene            complement(32918..34597)
                     /locus_tag="PKV56_04390"
     CDS             complement(32918..34597)
                     /locus_tag="PKV56_04390"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOX67082.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGIKVTLIKLDPYL
                     NVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFITTRMKKANNFTTGQIYKSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQEFVKRGAGVGTDQAVDVAIVEIGGTVGDIESLPFLEA
                     VRQMSLRMGPNNSAFVHLTYVPWIAAAGELKTKPTQHTAQKLREIGIQADVLLCRADR
                     RIPDEEREKISLFTNVPTWGVISMWDVDTIYKVPRILHEQGLDGLICDKLRINTPPAN
                     LTRWDDLVREFEHPERELTIAMCGKYTDLSDSYKSLNEALRHAGMHSHAKVNIEYVDS
                     EMLNESNVSQLSGYDAVLVPGGFGKRGVEGKILTAKFARENKLPYLGICLGMQVATIE
                     FARHQAGLAQANSTEFDPETPHPVIALIEEWQDADGSIQKRSTSSDMGGTMRLGAQSS
                     DVKPGTLAHEIYGDVVTERHRHRYEANVNYLDQLSAAGLVISALTQREKLTEIVELPR
                     SVHPWFMGVQFHPEFKSTPWNGHPLFNSFIQAALSHQRDRKSPGASMKAVA"
     gene            complement(34658..>35166)
                     /locus_tag="PKV56_04395"
     CDS             complement(34658..>35166)
                     /locus_tag="PKV56_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOX67083.1"
                     /translation="DFNASMIFGGQIKGEAMRLFLVYSAGNFIEATRETCYFQVGESK
                     YGKPVLDRMITPATPLDEASKCALVSMDSTLKSNLSVGLPLDLLVYENDALQCNQIVC
                     IDEHNPYFRMIRGSWGEKLRQVFESLDDPHWSNDTTAGATAAHPLCVPSNRYEPMRKI
                     THPGEKIV"
BASE COUNT         5729 a        11452 c        11879 g         6106 t
ORIGIN      
        1 gaaggcacgg tcgacttcat ccccaactac gacggctcga cgcaggagcc gcggcagttg
       61 ccgagccgcc tgccgttcgt gctgctgaac ggcgccagtg gcatcgcggt cggcttggcc
      121 accgagattc cgagccacaa cctgcgcgag gtcgcgctcg ccgcgatcgc gctgatcagg
      181 aacgaccgcc tcgacgacga cgaactcgcc gcgctgatcc ccggccccga ctacccgggc
      241 ggcggccaga tcatcagcag cgcggacgag atacgtgcgg cctatgccac cggccgcggc
      301 agcctgaagg tgcgggctcg ctggaagatc gaggacctgg cacgcggcca gtggcaactg
      361 gtcgtgaccg agctgcctcc gggcacaagc tcgcagcggg tgctcgagga gatcgaggag
      421 ctgaccaacc cgaaggtcaa ggccggcaag aaggcgctga gtgccgacca gctgcagctg
      481 aaggcctcgg tgctcggcgt gctcgacgca gtgcgcgacg agtcgagcaa ggacgcgccg
      541 gtgcggctgg tcttcgagcc caagaccagc cgcatcgagc agagcgagct gatcacgcaa
      601 ctgctggccc acaccagcct ggaaagctcg gcgccgatca acctgacgat ggtgggcgcc
      661 gatggccggc cgacacagaa gacgctgcgc cagatgctcg tcgagtggat cgcgttccgg
      721 ttgcagacgg tgcagcgtcg ctcgcagcac cggttggaca aggtactcga ccgcatccac
      781 gtgctggaag gccggcagtt ggtgctgctg aacatcgacg aggtgatccg catcatccgc
      841 ggggccgacg agccgaagcc ggcgctgatc gagcacttca agctcagcga gcggcaggcc
      901 gacgacatcc tcgagatccg gctgcgccag ctcgcgcggc tcgaggccat caagatcgag
      961 caggagctga aggagctgcg cgtcgagcag ggaaaactcg aggacatcct cggcaatccg
     1021 ggcacgctga agcgcaccgt catcaaggag atcgaggccg acatcaagca gttcggcgcg
     1081 gtcgagaagg acccgcgccg cacgctgatc caggtcgaga agaaggcggt cgtcgagatc
     1141 aaggtgatcg acgagccggt caccgtcgtc gtcagtgcca agggctgggt gcgtgcgctg
     1201 aaggggcacg aggtcgatcc ggccggcctg cagttcaagg ccggcgatgc gctgctggcg
     1261 acgttcgcct gccgcagtgt cgacccgctg ctgatcttcg gcagcgcagc caacggcagc
     1321 ggccgggtgt actcgacagc ggtcagtctg ctgccgggtg gacggggcga cggtcagccg
     1381 atcaccacgc tgatcgaact cgaagccggc acgcagcccg cgcactactt cgccggtcac
     1441 gcggcgcaga cgctgctgct ggcgggcaac gccggatacg gcctgctggc gcgtgtcgga
     1501 gatctgacgg cacgccagcg cagcggcaag gcgttcctga cgctggaagc cggcgagcaa
     1561 ttgctgccac ctgcgctggc ttcggcgggc atgctggggg atcaggtcgg ctgcctgtcg
     1621 tcgcaggggc gtctgctggt gttcccgctg tccgagctga aggtgcagcc caacggaggt
     1681 cggggactga cgttgatgga tctggagccc agggacacgc tcgccagcgt cgcggtgttt
     1741 gcccaggcgc tgcgcatcga gggcaccgga cgcggtggca aggcgcgcga cgaggtgttg
     1801 aagggggcgt cgctggctcc gtatctgggc aagcgggcac gcaaggggaa atcgcaggat
     1861 gcgataccca aggcacaaag gttgttgccg gcctgaggcg cccgatggga ggcccgttca
     1921 cattctcatg gggcatgtga acggacgcca tcctcgcttg aggccaactc gggtgcagcg
     1981 ttcaattcaa ggcagggagg cgcgacggat cggggctgtg ctcgggcctc gggtgtctcc
     2041 cgcaacccgg acgccaggag tcgccatgca cccatcgatc gttcaacgtg ccgatagcag
     2101 caacgctttc gcgcgtgtgc cgccagagcc acgcttcgtg ccctcgcaac tcagctatct
     2161 ccggccgatg tcggagtgtc cggtcagcta tgccttcgag ccaccgcccg gcacaccctg
     2221 ggagagcggc gagtacgagg aacagacggt gcagatcacc gatgcgcgca gtgcgccgca
     2281 ggccccttgc atcgatcacg aagggtacgt gctgagggat gctccaacgg cggtgacgaa
     2341 cttcctcgac gaggacgcga tccgaacgac ctactaccgc gaggcggccg agttggcgtt
     2401 ggcggtcacc ggagcccggc atgcgcatgt ctttgatcac ctggtccggc gccgggaggc
     2461 cgggcgtgcg ccgttgagct tcgggcggcg ggacgcgggc ggccgcgccg cggcgaacgg
     2521 ccgggtgcac aacgactact ccgacgcgtc aggaatcagg cgcctggcaa tggttttgcc
     2581 gcagtgcgat gttgcgcgag tcaggcgcta cggcatcgtc aacttgtggc gttcgatcaa
     2641 cggaccggtg caggacacgc cgctcgcgct gtgcgacgcg cgcacgctgt cggccttcga
     2701 cctggtcacc agcgaggttc gctatccgcg gcgtaccggc gagatctacc tcgcgaggtt
     2761 ctcggctcga caccggtggg cgtacttctc cgagatggat cggcacgagg cactggtgtt
     2821 caagcaattc gactcgcagc tcggtggggt cgcccgattc acgccgcatg cggccttcga
     2881 tctccctgac gtaccgcccg gcacgccgct gcgcgagagc atcgaggtac gctgcctggt
     2941 cgtctacgag caagaggatg gagcgtcatg agtgaggtca agacagggaa ggctccagcc
     3001 gacctcgagt tctggttcga attcggcagc aactacagct atctcagcgt gatgcgcatc
     3061 gaggcagccg cagcccggct gcaggtgccc atccgctgga agccgttcct gctggggccg
     3121 atcttccgca ccttgggatg ggacacctcg ccgttcgtgc tccagaaggc caagggcgag
     3181 tacgtgtgga aggacatgga acgcgcctgt cgcgacctcg gtttgccctg gatcaggccc
     3241 accacatttc cgcgcacggc actgttgccg atgcgcgttg cgctggtcgg agccgaacag
     3301 ccgtggatgg gcgcctattg ccgtcgcgtg atgtccctca actttgccga ggaccgcgat
     3361 atcgacgcgc ccgatgtggt ggccgacgtg ctgaagcagc tggggctgcc ggcccggcaa
     3421 atcatcgatg aggctcgatc cgagcccaac aagctgaagc tgcgtgagca gaccgaggcc
     3481 gcgcgtgccc ggggagtctt tggcgcgccc acgttccttg ttggcgacga gatgtactgg
     3541 ggcaacgatc gtctggacga cgccctggcc tgcgccagcc tgcggctcaa gccggagtga
     3601 cagcccgcga cgaggcggcc tccgcttcga tccacgcagc gacctggcga accgcgaccc
     3661 gtggttcccg ttcgccgcgg atcagccaat aggcgtgggt tgtctcgggc tgcttgtcgt
     3721 gtggcgcgag cgccaccaga cggtgagcgt cgagcagtgg ctggatcagc gacagccgtc
     3781 cgagcgcgac gccctggccg gccagcgctg cctgaatcac ctgatcgtac tggttgaagt
     3841 gcagcacgcc gcgcggccgg gtgtcggccc agcccttcgc agcgagccag tcttcccatt
     3901 gcagccaggg gtgcctcggg tcgtcgaact ccagcagcga tatcctggac agcgccttgg
     3961 ccgagccgag cgacctcagg cccaggccgg gatgcgcaac gggcatcagc gattcgccga
     4021 acaaccaggt ggcgccttct ggcgcggacg cggagtagcg gatggccaga tcgatgccgt
     4081 cgtggcgaag atcggcgacc tgattgttgg cggacacccg caggtcgacg ccggggtgcg
     4141 ctgcctggaa gtggctcaag cgcggaagca accacaggcc ggtgacgccg atgctggcgc
     4201 tcagcgtcac cggccgtgca gaccctctgg tactgatgtc acccagcaca tcctgcaact
     4261 gctggaccgc gccatcagcg cttcggaaca ggcgttcgcc ctcgggggtg aacgcgatcg
     4321 agcggtggcc gcgcaccagc agcttgacgc cgagtcgatc ttccagcgca cggatctggc
     4381 ggctcacggc cgactgcgtg aggcacaggt cctgcgccgc gagcgtgatg ctcatgcgcc
     4441 gacccaccgc aacatagcct cgaatcaggt ccagggacga caagcgaacg aggggaagtg
     4501 acattctcgt ggtgcatgcg gcggtgccgg aaaatcgatt gaagcacaag cggcccagat
     4561 gtgtaatggg gcgcatgcac accggcgtcc tgccggatcg ggatcgggct tgcagccagg
     4621 ccatgcatca cgggagcgtt tgtggattac gtgctgatca cccatgcggt caaggactac
     4681 gaagcctgga agcaggtctt cgacgatgcg gccccgatgc gaaagagcgc cggggagcag
     4741 cgttacttcg tcttgcgaca tgaggacgat gcgaaccgga tcgttcactt ttcccaatgg
     4801 gcatcgatcg accgggcccg gagcttcttc gagtcgccag aactggtcga gatccgccgg
     4861 ttggccggtg tcgagtcgcc tgaattcagc tacctgcgca gcctcgagga aggtgtgctg
     4921 tgaccacgcc tcgtcaacca cccgtctttg cacgtgccga tcaggtgcca cctcgagtca
     4981 aggcgtccag ctacccggag ccgtttgctt cccggatgca gggacgcacg aagcggccac
     5041 tcggagaact gttcgggttg aacaacttcg gcgtcaacct gaccacgctg aacccgggcg
     5101 cggtgtccgc gctgcgacac cagcacctgc gccaggatga gttcgtctac atcctgcagg
     5161 gtgaggcgac cctggtcacc aacgagggcg aaacgctgct gttgcccgga atgtgcgcag
     5221 gctttgcggc cggcaacgga gatgcacaca tgctgatcaa tcgcagcggt ctgtcgtgtg
     5281 tctacctgga gatcggagac cggacgcccg gcgaccacgc cacctatccg gacgatgatc
     5341 tggtggcgtt ctcgacgcag cagggcgcgt ggcaattcac gcacaaggac ggccagcctt
     5401 actgaccagg gtccgtcctt gtcgatgaag gatcgggcgg cgtgcgctga atcagtaacg
     5461 acggcgtggc ttgctcgcca ccaccaggat cgaagccgcc gccagcatga cgaacaacgc
     5521 caggctccag tacgcgtact gaatgccgag cagcttcact gcagcgtcag cgcagttggc
     5581 ggtgacctga agcagcggcg ggaatgcgcc gtcgagtccc agggcgctga tcaccttgtc
     5641 ggcaaaggtc aggttgcatg atgctgattt cgatgccacc aggtcctggt agagcgccga
     5701 ggccgcaccg ctgagtgcca gcagcagcac cagcacgccg atgccgcgac gcgcgacgat
     5761 ggacgaccac gctgcgccga gcaggcacag catcgcgatt gccaggtaga tgactcgctg
     5821 aaggacgcac caggggcagg gctgcatgtc gaagacatgc tgcgacacca gtgcgccagc
     5881 gacggatccg acgcacgcga gcgccatggc cagcagcagc cgggtcgttc ccgtggtcga
     5941 agacgacgag gccacggtca agcgacctgc acgatcagtt cgatctcgac acaggccccc
     6001 agcggaatct gcgccacgcc gaaggcactg cgcgcgtgtt ggcccttcgt gccgaacacg
     6061 tctcccagca gctcggagca tccgttggtc accaggtgct gctcggtgaa tgttccggtg
     6121 ctgttgacga ggctcatcac cttgacgatg cgttcgacct gatcgaggtt gccgatcgcg
     6181 gcgttcagcg tgcccagcag atcgatcgcg acggcgcgcg cagccagctt gccggtggcg
     6241 gtatccatgt tcaggcccag ctggccgacc cacggcttgc catcgcgctt ggcgatgtgg
     6301 ccggacagga acaccaggtt gccggtgcgt acgaacggca ggtaggccgc ggcgggaatg
     6361 gccagcggag gcagttcgat gcccagctcc ttcaggcggt cgtacacaga ggaggatgca
     6421 gtcattgggg ggtgtcgcga cagcgttgga atcaagctcc aaatcctaca ctgcggcgca
     6481 tgcctgcacc ccgtgccacc gatgccgcga acaccggctg gcgactggcg atgctgggag
     6541 cggcctggcc tgctggcgca gcagttcaat tgcgcgaagc gacgttgcag gcaatgccgg
     6601 tttacgtcgg atgcatggtc ctcgggctgc tgctcgttgc agcagcgacg cttgccggac
     6661 ggcgcagcat ggccgcagca ctcggcttga tggttctggc gttcggggtc accggtgggc
     6721 gggcgtcgat ccgactggcg gagacgctgc ccatcacgct ggaggcccgc gatctgcagg
     6781 tcacgggttg ggtctcgagc cttcctcagc gctcatcgaa cgggcttcgc ttccggttcg
     6841 aggtcgagca ggcgacgctg gacggtcaac cgatcaccgt gccgggtgtc gtgtctctgg
     6901 gctggtacgc aggcttccag gacggtgccg ggctgaccgc cgggcaactc cggctgcgtg
     6961 ccggtcagct ctggcaattc acggtgcggc tgcgccgacc gcacgggctg tcgaatcccc
     7021 atgggttcga tcaggagttg cggtggttcg aagacggcgt gcgtgcgacc ggatcggtgc
     7081 gggacgccac ggcgccccgg ttgctcgacg gcgcggtggc tcatcccatc gagcgcttgc
     7141 ggcagcacgt gcgcgatgcc gtggaggcgt cggtggtgga tcggcgcgcc gcgggcgtgc
     7201 tggctgcgct ggccgtcgga gaccagtctt cgatcgagcg cgatgactgg gacctgtttc
     7261 gccgcaccgg catatcccac ctggtttcga tcagtggtct gcacatcacg atgttcgcgt
     7321 ggctgtctgc ggcggccatc ggcgcactgt ggcgtcgcag tgagcgggcg atgctgtggc
     7381 tcgcggctcc ctccgcggga atgtggggtg gattcgcagt ggccgggttg tatgcggtgt
     7441 tttccggctg gggcgtgccg tcgcagcgca cggtgtggat gctcggcacg gcctgcctgc
     7501 tgcgcagcgc gggattgcgc tggccgtggc cgctggtgct gctggcggct gccacggcag
     7561 tggcggtgat cgatccgtgg gcactgctgc agccgggctt ctggctgtca ttcgcagccg
     7621 tgggcctgct gatgctgtcc ggacgagcgc agttcgaagc ctctggcgct gtgcccgctg
     7681 caagcgccga cccgttggca acggcaggca cgcgcgggcg ggcgctcggc ccggcgctca
     7741 ggggctgggt gctgggaggc gtgcgcacac agtggatcgc caccgtggcg ctgactcctc
     7801 tgacgctggt ctgtttccag cagatgtcgc tgatcggctt cgtggcgaac ctgattgcga
     7861 tcccgctgat cacgctggtg gtcacgccgc tggcgctgct gggctgcatc gcggcgccgc
     7921 tgtggacggt cggcgcgtgg ttcgtttcgc tgctctgcga actgctgggc tggatggcgc
     7981 aggcaccggc ctcggtctgg tcggtcgcgg ttgcgccgtg gtgggctcag gctgccgggc
     8041 tgttcgcagc agcgtggatg gtcacgccgt tgccttggag ggtgcgggcg cttgccgtgc
     8101 cattgctgct gccgctgctg ctgccagcgc gagatctccc ggctgtcggc cagttcgaac
     8161 tcgtggctgc ggacgtgggg cagggcaccg cagtcatcgt gagaacgcgc ctccatgtgc
     8221 tgctctacga cgccggaccg cagtattcgc cggagttcga cgccggacag cgcgttctgc
     8281 tgccgctgtt gcgagcccgt ggcgatcacc gcatcgacat gctggtgctc agccaccgcg
     8341 acaccgatca cgtcggcggc gcagccacgc tgctgcaagg cctgcccatc gaagagctga
     8401 gcagttccat cgaagatctg catccgctgc ggatcgacgt tcgcgcccgg ggcctgaagg
     8461 atggtcgttg cactggcggt cagcactggg agtgggatgg cgtgcgcttc gaggtgctgc
     8521 atccggcgaa cgacgcggac acgggcgggc tgcgacccaa caccgtgtcc tgcgtgatcc
     8581 gggtgcaagg cgcaggagcg agcgcgctgc tgaccggtga catcgagcgc gaccaggaga
     8641 tccgcctgct gcgcgagcgc cgcgagtcgc tcgccagcga tgtgctgctg gtgccgcacc
     8701 atggcagcag gacctcgtcg tcgccggctt tcctggaggc ggtcgatccc cgggtggcgg
     8761 tctttcaggc cgggcatctg aaccgctacg gccacccggc tgccgacgtg ctggcgcgct
     8821 atcgcgaacg tgccatcttc gctgtcgaca gcccgaactg cggcgcctgg acctggtaca
     8881 gcgcgtctcg caacgacaat ccgatggctg ggtgtcaacg cgtgctggca cgtcgctact
     8941 ggcagtggcg ggcgtcggat gcgccctgac ctcaggttgc gcgcatgatc ggaaggtgcc
     9001 ggttctcgcc ggcacggggc ggattcgggg ttcctgaccc cgtgtttcat ggcccgccac
     9061 gccgctgctc aagaccccaa tggcctggaa attgcttccc tgtgcttcag gagtgatgcc
     9121 gatgagatca agttttgacg agatgcaaat ttccagcact caggtgcggg agcattaccg
     9181 tggctacgag cagtggctgg cacagcaacc cggcgatgtg atgaagtcgc gccgcgaaga
     9241 agcggagatg atcttccgtc gcgtcgggat caccttcgcc gtgtacgggg cgaaggacga
     9301 ggacggttcg ggcaccgagc gcctgattcc gttcgacctg atcccccgcg tgattccagc
     9361 gcatgaatgg gtcgacatgg agaagggcct gcgccagcgc gtggtggcct tgaaccggtt
     9421 catccacgac gtctatcacg gccaggacat cattcgcgcc ggcatcgtcc ccggcgatca
     9481 gatcttcaag aacgcgcagt tccggcccga gatgatgggc gtcgacgtgc ccttcggtgt
     9541 gtactcgcac atctcaggca tcgacatcgt gcgcgccgga aatgccgatg gcagcggcag
     9601 ctactacgtg ctcgaggaca acctgcgggt gcctagcggc gtcagctaca tgctcgagaa
     9661 ccgcaagatg atgatgcggc tgttcccgga gctgttcagc cagcatcgag tcgagccggt
     9721 tgcgcactat ccggacatgc tgctcgaaac gctgcgcgcg gtggcgccgg cctcggtcaa
     9781 cgagccgacc gtcgtcgtgc tgacaccggg catgtacaac agcgcgtact tcgagcatgc
     9841 gttcctggcg cagcagatgg gcatcgaact ggtcgagggg caggatctgt tcgtcaagga
     9901 caacttcgtc tacatgcgca cgacgcaggg accgaagcgg gtcgacgtca tctaccgccg
     9961 tgtcgacgac gactttctcg atccgacggc cttccgcccc gattcgacgc tcggctgtgc
    10021 agggctgctc ggtgcctacc gggccggcaa cgtgacgctg tcgaatgcga tcggaaccgg
    10081 cgtcgccgac gacaagtcga tctacccgta tgtgccgaag atgatcgagt tctatctcgg
    10141 cgaaaagccc atcctgaaca acgtgcccac ctacctctgc cgcgagaaag acgatctgaa
    10201 atacgttctc gatcacctcg ccgagctggt cgtgaaggag gttcacggcg ccggcggata
    10261 cggcatgctg gtgggtcctg cgtcgacgaa ggccgagatc gccgagttcc gcgccgtgct
    10321 cgaagccaat ccgtcgggct acatcgcgca gccgacgctc gccttgtcga cctgcccgac
    10381 cttcgtcgag tcgggcatcg cgccgcggca catcgacctg cggccgttcg tgctcagcgg
    10441 caagaccgtg cagatggtgc cagggggtct cactcgcgtc gcgctgaagg agggctcgct
    10501 cgtcgtcaat tcgtcccagg gtggtggcac caaggacacc tgggtgctgg aggactgatc
    10561 atgctgtcac gtaccgccga ccacctgttc tggatggctc gctacatgga gcgggccgag
    10621 aacaccgccc gcatgctcga cgtgaattac cagacgtcgt tgctcccgca atccgccgac
    10681 gccgcagagc agggctggaa gggcctgctc agcatttccg agctgagcgg cgatttcgcc
    10741 gcgcgctacg ggaaggtcac cgcgcgcagc gtcatggagt acatggtcag cgatccggaa
    10801 aacggatcga gcatccgtaa ctgcctgatg gcggcccgcg agaacgctcg cgccgtgcgc
    10861 ggaacgctga ccaccgaagt ctgggagaca cagaaccaga cctggctgga attccagcgc
    10921 atggtggcca acggggcttt cctcaaggac ccgggtaccg cattcgagtg ggtgaagttc
    10981 cgatcgcacc tgtcgcgcgg cgtcaccgtc ggcacgatgc tgcaggacga ggctttccac
    11041 ttcctgcgca tcggcagctt cctcgagcgc gccgacaaca cggcccgatt gctcgacgtc
    11101 aagttccacg ctgtagaaag cgaattcttc ggcggaccgc acagcatggg atcgacggcg
    11161 ccgtcggctc cgggcgaagc ggaatcgagc ggatcgagtc aatcgcaggg ctcaccggcg
    11221 cgcagcggcc gggacgtcga gtacgacttc tatcactggt cggcgatcct gcggtcggtt
    11281 tcgggattcg aggtctatcg caaggtctat cgaaacgtga tccggcccga gaaggtcgcc
    11341 gagttgctga tcctgcggcc ggacatgccg cgttcgctgg ccgcttgcac ccaggaggtc
    11401 ctttccaatc tgacaatgat tgccaacgag cactcgagcg aaaccttgcg ccgtgcgggc
    11461 cgccttcggg ccgacctcca atatggccga atcgacgaaa tcctggcgac cggtctgcac
    11521 gcctatctga cacagtttct ggaacgcgtg ggaacgctcg gcgtgggcat cagccgtgac
    11581 tttctcgttc cagtggagtc ctgagaatgt ccatccatgt cgcgctgaac catgtgaccc
    11641 attaccggta cgaccggccc atcaacctgg gtccgcagat cgtccgcctg cgtccggcgc
    11701 cgcattcacg cacccgcatc ctgagctact cgatgcgcgt ggagcccgcc acgcacttca
    11761 tcaactggca gcaggatccg caatccaact acctggcccg gctggttttc ccggacaaga
    11821 cgacctcctt ccgcatcgag gtcgatctgg ttgccgagat gtcggtgctc aatccgttcg
    11881 acttcttcct cgaaccgtcg gcagagaact tcccgttcca gtacgaaccc gaactggcgg
    11941 ccgaactcgc gccctacatg gccaagggcg agctgactcc gctactcaag aagtacgtcg
    12001 acagcgttcc gcgcacgaag aagcacacca acgatttcct ggtcgaactg aaccagaaac
    12061 ttcagaagga catcagctac ctgatccgca tggagcccgg ggtgcagacg ccggaagtca
    12121 cgctgaccaa cgcatcgggc tcgtgccgcg acaccggctg gttgctggtg cagatccttc
    12181 gacacctggg cctggcggcc cgcttcgtct cgggctacct gatccagctc aagagtgatg
    12241 tgaagtcgct ggatgggccg agtggcaccg aggtcgattt caccgacctg cacgcctggt
    12301 gcgaagtgtt cctgcctgga gccggctgga tcggtctcga cccgacgtcc ggtctgctgg
    12361 ccggagaagg ccacatcccg ctggcctgct cgcccgatcc atcctcggcg gctccggtca
    12421 gtggcgccat cgatgaatgc gagaccgaat tcgagcacca catgagcgtc acgcgtgtgt
    12481 gggaggcgcc gcgcgtcaca ttgccgtaca ccgacgctca atgggagagc atcgaggcac
    12541 tgggtcaccg tgtcgacgag gatctgcgcc gcaacgatgt gcgcctgacg cagggcggcg
    12601 agccgacctt cgtttcggtc gacgatcgcg atggcgccga atggaacacc gaggccatgg
    12661 gcccgaccaa gcgcctgctc agtgccgacc tgatggacaa gctgcgcgcc aagtacggcg
    12721 acggtggctt gctgcactat ggtcagggca agtggtaccc gggcgagcag ttgccgcgct
    12781 ggtccctgaa cctgtactgg cgcaaggacc gcgagccggt gtggacccat cccgagctgt
    12841 tcgccagcga gcacaaggac tatcaggtca ccgacgtgca cgcacagaag ttcctgagtg
    12901 gcgtggcgcg gcgactggcg ctcgatccca agtacgtgtt cccggcctac gaggacactt
    12961 ggtactacct gtggcgcgag cgcaagctgc cgaccaacgt cgatccgttc gatgccaggc
    13021 tgtccgatcc gctcgagcgt gagcgccttt gcaaggtgtt cagccagggg ctcgacaagg
    13081 ttgtcggcca tgtgttgcca gtggcacgcg gcacccacac gcaggcgcgc tggcagaccg
    13141 gaccgtggtt cctgcgtaca gagcgctgct acctcgtgcc cggagattcg ccactcggct
    13201 tccggttgcc tctcgattcg cagccctggg ctgccgaagg tgacatgccc tgggtttatc
    13261 cacccgacca gacgcagcag ttcgttgcgc tgccaccata ccgtcgcctg cgttacgcga
    13321 acggctcagc ggagggagcg ggatctcccg ccggcccggg ctcggcggcc ggaaccggcg
    13381 ccggttttgc agaacgctcg cgcgctgcgc agggccccga gaaggccgtg gccagcgcac
    13441 ccgcgcccag cctgcgtgtg ccatcgagct tcgagtctgc gggctggatc agccgcacgg
    13501 cgctttgtgc cgagccgcgc ggagggcgcc tgtacatctt catgcctccg atgtcggtgc
    13561 tggaggatta cctcgaactc gtctccgcca tcgaggacac ggcggccgag ctgaaggtgc
    13621 cggtgatcct cgaaggctac gagccgccga aggatccgcg cctgtcgacg ctgcgggtca
    13681 cgcccgatcc gggggtgatc gaggtcaaca tccatccggc gcattgctgg gaagagttgg
    13741 tcgatcagac gacgcatctg taccagagcg cgcatgaaac ccgcctgtcg accgaaaagt
    13801 tcatgctcga tggccgacac accggcaccg gcggcggcaa tcacttcgtt ctcggcggcg
    13861 cgacggtggc ggactcaccg ttcctgcgcc gtccggacct gctcgccagc atggtcgcct
    13921 actggcacaa ccacccggca ttgagctacc tgttctcggg cctgttcgtc ggaccgacca
    13981 gccaggcacc gcgtgtcgat gaagcacgca acgattcggt ctacgaactc gaggtcgcct
    14041 ttgccgaact gcaacgcgtg acgcgcgaga acggtgacta ctgcccgccg tggctgatcg
    14101 accgactgct gcgcaatctg ctgatcgacg tgaccgggaa cacgcaccgc gccgagttct
    14161 gcatcgacaa gctgtactcg ccggacggac cgacgggccg cctgggtttg ctcgagatgc
    14221 gtgccttcga gatgccgccg catgcccgca tgagcctgac ccagcagctg ctgatgcgcg
    14281 cgctggtctc gcgattctgg aaggaaccct atcgcccggc ccgcctgaag cgctggagca
    14341 ccgagctgca cgaccggttc atgctgccgc acttcatctg gcaggacctg tgcgacgtgg
    14401 tcgaggaact caacgcattc ggctatgcgc tgaaggccga ctggtttgcg ccgcacctga
    14461 acttcaggtt cccgcgactt ggcgatttca ccgcgatggg caccgaggtc gagttgcgcc
    14521 tggctcttga accatggcat gtgatggggg aagagaacgc tgctggcggc acggcgcggt
    14581 tcgtggactc ctccgtcgag aggatccagc tcaaggccac ggggctgatc agcgaccgtc
    14641 atgtgctcac ctgcaatggc cgggtcgtgc cgatgcagcc caccgggcag gtcggggagt
    14701 tcgtcggtgc agtgcgctac cgcgcctgga acccgccatc tgcacttcac cccacgatcg
    14761 acgtgcatgc gccgttgacc ttcgacctgc tcgacacctg gatgagtcga tcgatgggtg
    14821 gctgccagta ccacgtggcc catccgggcg ggcgcaacta cgacaccttc cccgtcaacg
    14881 cctacgaggc agaggcgcgg cgcctgaatc gcttcttccg caccggccac acgccgggtt
    14941 cgatgcaggt gccgaaggat gagcgcaacc cgaacttccc gttcacgctg gacctgcgtc
    15001 gggcgccgtc gtcgacctga cccggccatt ccggggccac gtgtgaatgc gggccccggg
    15061 ttttcacatc ggcgttgcga cccgtcggtg accaaacccg cggggcccgc gtatgacaat
    15121 ggacccgatg aagttggttg tctgacatgt tgagtcgtct gttatccggt tatgcctcgg
    15181 tcgaggggcg ctatgacgag ttgctgtcgg cgcccggcat tcctcgacca cactgggatg
    15241 cgttcctgcg ctcgctggcg gcgcgccagg gtctcgaggt gagcgagacg ctggcgctga
    15301 tggaaagcga gatccgcgag aacgggatca cctacaacgt ctacgccgat ccgaaagggg
    15361 ccgaccgacc gtgggaagtc gatccgctgc cgctgctgct gtcggccacc gagtgggacg
    15421 aaatcgccgc cggcatcgca cagcgggccg atctgctgaa ccgggtgctg ggggacatct
    15481 acggaccgca ggaactgctg cgctccggtg cgattccgcc atcgatcatc ttcggccaca
    15541 gcggctacct gcatcaggtg cagggcatcc gccccccggg cggtgtgcac ctgttcaact
    15601 atgcagccga tctggcgcgt tcacccgatg gtcactggtg ggtggtcaac gatcgcacgc
    15661 aggcaccgtc gggatccggt tacgcgctcg agaaccggct ggtcgtttct cgcgtgtttc
    15721 cccaaatgtt ccgcgatctg catgtgcagc acctcgcatc cttcttcgat gcgctgcgtg
    15781 aatcgctgct gcgctgggcg cccaaggaca tggccgaccg cgcttccgag cgggccaatc
    15841 ccctggttgt cctgctcacg ccaggtccgt acaacgagac ctatttcgag catgcactgc
    15901 tggcgcgcta cctgggattt ccgcttgtcg agggcagcga tctgacggtg cgtgatgggt
    15961 gtgtctggat gaagacggtc gaaggcctga agcgggtcaa tgcgatcctg cggcggcagg
    16021 acgacgacta ctgcgatccg ctggagctgc gctccgattc ggcactgggc gttcccggac
    16081 tgaccgattg cgcacgccgt ggcacggtgc tgctggccaa cgcgctcggc tcgggcgtgc
    16141 tggagtccgg cggcttgctg ggctacctgc ccaagctcag cgagcaactg ctcggggagc
    16201 cactgcgatt gccttcggtc gccacgtggt ggctggggga gccggccgcg ctcgacgacg
    16261 cctggagccg cctcgacaag ctcatcatca agcccctgga gcgatcgatc cgcgagccgg
    16321 cggtgttcgg cgccgacctc tcggcgaaag agcgtgcagc cctgcgcgag cgcgtggcgg
    16381 cgcgcccgca gcgctacgtc gcccaggagt gggtgcacgt atcgcaggcg ccggtgatcg
    16441 aacaaggatc cgaccgccag cgcgctgaac agcttggttc ccgcaccgtc gggctgcgtg
    16501 tctttgcggt ggcgacgccc agcggctacc acgtgatgcc cggcgggctg acgcgcgtgg
    16561 cgggtgacga ccagtcccgc gtcatcgcca tgcagcgggg cgggcgctcg aaggacacgt
    16621 gggtcctgtc ggacgcgccg gtcaacgcct cgttttcgtt gctgagccgc accgtcaagc
    16681 cccaggatct cgtcgcgtcg cgcgccaacg tgccttcgcg cgcggccgag aacctgttct
    16741 ggttcggccg ctacggagag cggtgcgagt ccgcagcccg cctcctgcgc gtcgcgatcg
    16801 gcgatgtgct ggacgattcc cgtgaatccg cggacggcgt tgcaccgacc ttgatcctgg
    16861 ctcagcgctt cggactgatc gacaacaccg ataaccccag caccgatctg ctgcgagcgg
    16921 ccactcatcc cgacgagggc ctgagcgagc gcctgcggca actttcgcgc gtggcgttca
    16981 acctgcgcga tcggatgtcg gccgatcact ggcgatcgct caaccagctg atttcggatc
    17041 cggtgttcca gcgcggcctg tcgtccacca gcctgggact gccgctcgcc atgggctggc
    17101 tggatcgcgc ggtgacatcg atgatgacgc tctccggatt cgttctcgat ggcatgaccc
    17161 gcggcgtggg ctggcggttc ctgtccatcg gccggcgcat cgagcggctg tcgacgttgt
    17221 gcaatgttct gcaggtcgcc atcaacgaag gccgctcgca cgggctggac tggctgctgg
    17281 agtacggcga ctcgacggtc acctaccgct cgcgctatct cgttgctccc gagtggcttc
    17341 ccgtgctcga tcttctcctt cgcgacgagg tcaacccgcg ctcgatcgcg ttccaggtca
    17401 agggtctggc cgagtacgtg gccaagctcg agctcagtca cggacgtttt gccagcgacg
    17461 tgctggcgcc tgcgcactac gctttgcgca atctcgatac cttggatctg aaccccgaaa
    17521 gcgaggcgct ggccgatgtg ctggtgcaac tgcagcgggc ggcacacgcc gtgtcggacg
    17581 agttgacgct gaagttcttc tcgcacgccg cgtcgcgcag cgtcttgtct ctggtggcct
    17641 gaccaccttc gcgcactggg ccagatgtcg caacagcaat ctcagcagca acagcatccg
    17701 tcgatggtgt tcgaccgcta cacggtcgag cacgagacgc gatatgccta caccgcgccg
    17761 gtgtcgcaat cgtggcagct gggtcgcctg acgccgcgcg tgctgccatg gcagcggctg
    17821 gtctcgcaca gcctgcagat cgaaccgtca cccgacgagc gtcattcagc cacggacagc
    17881 ttcggcaatt cgatcacgca tttcgggctg cacggcgccc accgccagct gaccgtgcgc
    17941 atgcagtgcc aggtcgaggt cggggatcgt ccggctgtcg atccccgtga atcgatgacg
    18001 gtcgacgaag cgcgcctggc agtgctgcgc cgccagaacg ctgccgagga tctgggcgct
    18061 gcgcgcatgg ccgagccgac gctgctggtg cctctgtccg aagcagcacg gatctatgcc
    18121 gcttcgtcgc tgagacctgg ccgcccgtgg gtcgaggcgc tgaccgatct gacgcaccgc
    18181 atccacaggg atttcgagtt cgatgccggc gcgacgacgg tcagcacctc ggtcgacgag
    18241 gtgatgcagc accgccgcgg cgtctgtcag gactttgccc acctgatgct ggcctgcctg
    18301 cgcggtcacg gcctgtcggc ccgctacgtg tccggctacc tgttgaccga cccgccgccc
    18361 ggcatgccgc gactgatggg cgtcgacgct tcgcacgcct gggtggctgc ctacctgccg
    18421 gggcagggct ggatcgagtt cgatccgacc aacgaccagc tcgccgaccg gcgctacatc
    18481 acgctggcct ggggcgccga cttcgccgac gtggtgcctc tgcgcggcgt gatcctgggc
    18541 ggcggcatga tgcagggcat ggacgtgtcc gtcagcgtga tcccgatgac ctgaaaaggt
    18601 gggaccaccc ccgcccccat ttccgggtgg tgcttcaagg ggtgcgcatg tagcccgttc
    18661 tcgacccctc ggcgggatgg tcggcgcaca cgctccccgg aaaactgacg tcatcgatca
    18721 agccgatcga cgaccgttca ggagcccgcc atgttcagca ccggttcaac gccaggatca
    18781 agcgacctca tcgcacgcgc gatcgtgttt ctctcgctct gcccggcatt tccgctgccc
    18841 gcccggtccg tcgagacgct ggtcgaagct gcagagtccg ccgtccgcgg cccgggctgg
    18901 tacgactcga gctgggagct gagctgcggg cttgacatcg ccgaggtgcc gagcctggac
    18961 gactggattg gagtctgcca gggtgacgcg ccgctcagcg cgcacccttg accgcggtcc
    19021 ccagatcgat caccgccaga gcgtagtagc ttgaccagtt gtaccgcgtg atcacgtaga
    19081 agttggccgt gccggccacg tggctcgggg cggcatcgcc gttctgcagc tcgacgaggg
    19141 cgagcaggcc gacatcgtgc gcggcgccct tgccatcgtc tgcagtgccg gcatccgaag
    19201 ccgccaggtc cggtgccctg gccccgaaga ctgcgccgtg ctcgacgaac acctgtgccg
    19261 tgaagctggg aacgatgtcg ggtgccagca gcagggcccg ttcgccgggg tcgctgggcg
    19321 gggtcacctc gaaatgcgtg ggcagaccgc gctgccagcc gaactcggcc aggtaatggg
    19381 ccacgctgcc gatcacgtcc gcggcgctgc cgtgcaggtc gatgtgaccg tctccgtcga
    19441 aatcgagtgc gtacttgttg acgctgctgg gcatgaactg cggcatgccc agggcgccgg
    19501 cgtagctgcc cttgagggcc agcggatcgc tgccctcgcg ctggccgagc accagcagct
    19561 gttccagttc gtcgcgaaag aaggcgctgc ggtccttgcg ttccctgggg aagtcgaatg
    19621 acagcgtggc cagcgcatcg atgacgcgga agctgcccat gtgtcggccg tagagtgttt
    19681 cgacaccgac gatgccgacc acgatctcgg gcggcacgcc gtagcgctgc tcggcccgtg
    19741 ccagccaggt ttcgttgaca cgccagaact cgaccccggc ccgcacccgc cggggctcga
    19801 tgaagcgtgc gcggtacgcc gcccagttct tggcggtgcc ggtcggcggc ggcatgatga
    19861 acttgacgac atcgggcacg aaccgggatt gagccagggt ggcctgcacc gcctcgacat
    19921 cgagtccctg ccgcgctgcg acctcggtgg cgaactgcat cacgtcggcc cgtcggccgt
    19981 aggtgacgat gtccggatcg gcatccgatc gcaccttgct tctcggtttt gccggatgca
    20041 tcttcttggc cgcgccgggc ttggcagcgt gagtccccgt gccatcggcg gcagggctgt
    20101 gcgtgggcgc gcacagcagc aacgcgagac tcagggcatg ggcccagaga cgggtcggcc
    20161 gtgtcgatat ttcaggtggc cggtggccca tgggacgaac agtgtcgtca ggcagaaagt
    20221 acatcgatgg attattgatt gcgcgctgtc ggcgaaacgt ctgccgccat cggcttcagc
    20281 cgacgcgcca gggccaggaa ctcgcgcgtc agacggccgt ccgggcgaga cgtgcactcc
    20341 cgaccgtagc gctgcacatc gatccggtcc agcatcaccg cgagcggctc gccggcctcg
    20401 ctgagctgcg ttcggacccg ttttgccagc gtgcggggcg cgtcgtgatc ggcggcaccg
    20461 atgccgaggc ggcgcagggc cgcgcgcagg cgatcgagtt gccgcaccca gggatcggtg
    20521 tggtggcgct cccaccacgc ccaggcggcg ccggccagcg ccagcaggct gaacgcgcca
    20581 accagcagca gcgccaggtc ttcccaccct ggagcgctga aacccagtcg ctgcaacagg
    20641 tcgagctggg agcctcgcga atagctcagc acccattgat tccagcggtt gttcagcgcc
    20701 tcccatccgt cgcgcagacc cgccagcaac tgcgggttca cgccgttcag tgcgccggcc
    20761 atcagcccgg gcggtgtcac gagtggtcgg ctgcgcagca cccggtcggg cgcgacggca
    20821 gaggtcgggt cggcgcgcac ccagccgaca tccgcctgcc agtattcggc ccaggcatgg
    20881 gcatagctct ggcgcacgat gcggtagccg tcgaccggcg tcgggtccag accctggtaa
    20941 ccggtgacca cgcgcgccgg cacgccgagc gcacgcatca cgacgacgaa ggcggctgcg
    21001 taatgttcgc agaatccgag cctgcgatcg agccagaact cgtcgatcgc gctgcgcgga
    21061 tcctgttctc cgtacagacc cggcgtgagg gtgtagccat agccacccgt gcggatgtgc
    21121 gacaacagca cctgtgccag cgtggtcccg ttggcctgag cgtaccgagc gtccctgcgc
    21181 agcgcggccg cccaggccag cgtgcgcggg ttgtgtccag gcggcaggtc gatgtagtcc
    21241 tgcaggccga tcaccggctg ctgcgggcca tgggtgaagc gcgggtaggc gatgcccgtg
    21301 aacctgatgc gttcgtacag tggtcgttcg gccacccatt ccagatcgtc gtgctgcgcg
    21361 actcgcacgc cctcgaccac ggggcctgac gggctggctt cgagcatcgg gatcacggcc
    21421 agtcgcagcg gttcgagcgt catctcgtag cgcacgggcg ctccctcgac gcgcaggtcg
    21481 gcgcggggtg tcagcggggg cgggaaggtc ggcttcagcg gcgtccagtc gcggccatcg
    21541 aaatgaccca gcaccgggcc acggaagtac acgtcctgcg gtgcgggagg tgcgccctcg
    21601 aaacggatgc gcagcgcaat gctgtcgtcc tgggccaggg cggccacggt ccccatgctc
    21661 atcacgttcg acagaccggt gctcgacagg ccatcctgcg gcacgcccca cagcggcccg
    21721 acccgaggga acagcaggaa cagcagcgcc atcagcggcg cacccagcag tgcggtcttg
    21781 cccgacagcg cggcggcctg gcgcaggctg ggcgagcccg ccggcatgtg ggcgagaacc
    21841 agcgcggtca gcagccccca gatcgacacc agcatcgcgg cggcgaccag aaggctctgc
    21901 gagtagagga actgcgtcag gatcaggaag aagcccagga agaacaccac gaacgcatcg
    21961 cggcgggcac gcagttccag cgtcttcagg gccatcaacg ccaccgccat cgtcacgccg
    22021 ggctccttgc ccagcagcga gcggaaggac cagaaagtga ggcccgtcgc gatcaccagc
    22081 acgccgagca ggacccagcg ccccggcagg gcggctccga ccagcgccag ccgcgctcgc
    22141 cacagcagca cgactcccgt gagcaccgtg caccatgttg gcaaatgcga caggtgcggc
    22201 agcaccgtcc agccgatcac gaccagcagg aacagcgtgt cccggctgtc ccgcggcagg
    22261 tgggcccaac cgggccacgc gaagctgcgc gtcttcatgg agaccacagt gccagtgctt
    22321 cgagtgcgcg gcggcgatgc gcttcgccct gatcgatcgg gatctcgcac cccggaaggc
    22381 tcaggcccca ggccgcgtcg cgtcgatcgg cctcgatgat ccacgcggtc agccgcgaca
    22441 ggcggccttc ggtgccgccg atgccacagc tctgccagtc gaaccacaac tgctgctgac
    22501 tctgggtgct gctgtcccgg ctgaccagat cgccgccgct ggccatcgcc ttggcggcct
    22561 gtttccaggc gatgcccttg agaggatcgc cacgccggta gccgcggatg ccctcgaact
    22621 cgccgctgct gctggcgtgc gactgaaccg taccgcccgc cacgaggcgg gtcgcaggca
    22681 gcgcgcttgc cggacgctcg ggctgcggat acacgacctg cgacatcgcc ggacgccaga
    22741 ccgcccaggc gcgaaacagg ccgagcggga agcgcgtctc cgcgttgagt gtcggcagcg
    22801 catgctgccc gcgttgtggc gggacgtagc cgagatgcag cgtcgcctgg ccgcctgcgg
    22861 gaacgttggt gtagcccaat gtcgacgcgc tggcatcctg caatcgaaga cccacgccat
    22921 agcgtgcgct gcccgggctc gacagcaccg cttcgagtcg tgccgtgtcg ccggcgaaga
    22981 ccgggccgac cggcctcagg tgcagcgtca ggccgcgcag cgtgttgtgc gtcatgtgca
    23041 tcgacaccac accgctgccg gccagcagga aggtcagcag gtagccgagg ttcagctggt
    23101 agttgatcga cgcgatcagc agcaccccca gcgtgaagca gaacatcatc ccggcgcgcg
    23161 tcggcaggat gtaaacattg cgctgcgtca gcagcaggct gtcggagcgc ggcgagcggg
    23221 attgccacca ggcatcgacg cgacggcgca gccgcccccg gaggcgggcc gtcggggtca
    23281 cggcagcggc gtggcctcga tcatcgcgcg cacctgctcg gcgcggccgc ggccggcgcc
    23341 cggtaccggg atcaatcggt gcgccgtggt ctgaaccagg atcgcctgga tgtcgtcggg
    23401 tgccacgtgc tcgcgctggt ccagcaacgc gtgcgccttg gctgcccgca gcaccgcgat
    23461 gccggcgcgc gggctcagcc cctctgtgaa ccagacccct gagcgcgtgg cggcaatcaa
    23521 cgcctgaagg tagtcgagca gtgcacccga tgcgtgcact gccagcaccg cctgctgcgc
    23581 ctcgatcagg cgctccgggc tcatcaccgc gcgcagccgg cccaccgcat cgcgccggtc
    23641 ctggcccgcg agcagctcgc gctcgctggc gcggtcggga tagcccagcg tgatgcacat
    23701 caggaagcga tctagctgcg actcgggcag cgggtaggtg ccgagctgat cggtcgggtt
    23761 ctgcgtcgcg atcacgaaga aggggcgggg caacggccgc gtctcgcctt ccacggtgac
    23821 ctgctgctcc tccatcgcct cgagcagcgc gctctgcgtg cgcggcccgg cgcggttgat
    23881 ctcgtcggcc agcagcacct gtgcgaacac cggcccgggg tggaacacga aggtctgcgt
    23941 cgagcgctcg tagacactga ccccgatcag gtcggtgggc atcatgtcgg cggtgaactg
    24001 catgcgcgag aaacgcagtc cgagcgacac cgacagcgca tgcgcgagtg tcgtcttgcc
    24061 gacccccggg acgtcttcga tcagcaggtg gccaccggcc agcaggcacg ccacgcaatc
    24121 acggatctgc tcgcgtttgc ctacgatgat cgtgctaatc tgatcgatca gtcgcgcgag
    24181 ttcctcggtc aggcggtggg cgggtacggc gttcatcgca ccaacgttaa ccgatggaag
    24241 gcgcggtttc actttccgga tcagcatgcc gaccgccttc tacagccacc ccgattgccg
    24301 aggccacgac atgggacgcg gccaccccga gtcgccgcaa cgcctcgacg cgatcgacga
    24361 tcacctgcgc gcggtcgggc tcgacatcgc gctggaggcg cgtgacgcgc ccctcgtcga
    24421 cctcgccgat gtcgccctgg cgcatgacag cggatacgtg accgagatcc agcatgtgct
    24481 gcagcaggtc atggccgatg gcgagccgcg ttccctcgac cccgacacca tcgccagtcc
    24541 gggcacctgg aacgcagtgc gccgggcggc aggtgccgcc gtggcggcga cggatgccgt
    24601 catcgacggc aacgcacgca acgcgttctg cgcagtgcgc ccgccggggc accacgcgac
    24661 acgccatgcg gccatggggt tctgcttctt caacaatgtt gcgatcgcgg cgcgtcatgc
    24721 ggtcgacgtg cgcggactgg agcgcgtggc gatcatcgat ttcgatgtgc accacggcaa
    24781 cggtaccgag gacatcatcg ccggcgacga gcgggtgctg atggtcagct tctttcagca
    24841 ccccttctat ccgtacagtg gcggcgtgcc gatggggccc aacatggtca atgtgccgat
    24901 accggcgtac acgcgcggtc cggaaatccg ctcgctgatc gaggccaact ggatgcccag
    24961 gctggagtcc ttccgtcccc agatgctgtt catctcggcg ggcttcgatg ctcaccgcga
    25021 ggacgatctc ggccagctcg gcctggtcga ggccgactat gcctggataa ctcgccgtct
    25081 gatggcggtg gcccaacgcc attcgaaggg acgcatcgtg tcgtgtcttg aggggggcta
    25141 caacctgagt gctctcgcgc gcagtgtcgc ggctcatctg cgggtcctgg cgggcgtcga
    25201 cggcgtcgaa tcgtcggagc cgcacccttg acccgcccgc tggactccgc cgaacttcag
    25261 gacctcgtcg acgcactttt ccagccgagc gcgctgaccg agctcggcgc gctgctgctg
    25321 tgtctggcgc tggcctggtt gctggtgcgg ctggtgcgcg gacaccctcc catcgatgcg
    25381 ccgatctggt tcgggcgtcg catcgtcgac ggtgtgctgt tcccgctgct ggcactggtg
    25441 ctggctttca gcgcgaagtt gctgctgacc ggtgtcgtga aggcggcggt cttcaagctg
    25501 gcgataccga tcctgatgtc tctgctggtc atccggctga ccgtgcgtgt cctgagcgtc
    25561 aactaccccg aatcccggtg gatgcgcatc atcgagcgca gcatttcctg gatcgcctgg
    25621 ctggcggtcg tgctctgggt caccggcgtg ctgcctctgc tgctcgaagc gatggacgag
    25681 gtgcgctgga tggccggtgg caccgagctg tcgctgcgca acgtggtcga gggtgcattg
    25741 accatcgggt tggtgatggt gctggcgctg tgggtggccg cagcgatcga gaggcgtctt
    25801 ctgagcggca gcggcaacga cctgtcgatc cgcaagatgg tggccaacat catccaggcc
    25861 ctgctgctgt tcgtcggcct gctgttcgcc ttgagcgtgg tgggcatcga cctgacggcc
    25921 ctgagtgtgc tgggaggcgc gatcggcgtc gggctgggct tcggactgca gaagatcgcc
    25981 gccaattacg tgagcggctt cgtgatcctg gccgagcgct cgctgcgcat cggtgacatg
    26041 gtcaaggtcg acaacttcga cgggcgcatc accgatatcc gcacacgcta cacggtgatt
    26101 cgtgctctca acggacgcga ggccatcgtg cccaacgaga tgctgatcac gcagcgcgtc
    26161 gagaactcgt cgctggccga tccgcgggtc ttgctcacga ccacggtaca ggtggcctac
    26221 ggcaccgacg tgcgtgcgct gcagccactg attgcgcagg ccgtggctgg cgtcgcgcgc
    26281 gtgctgccgg acccgacacc gacggtgcag ctggtcgagt tcgccgccga tggcatgaac
    26341 atcctggtca atttctggat cctcgacccg gagaacggcc agggcaatgt gcgttccgaa
    26401 gtgaatctcg cgctgctggc cgtgctcgac gagcaaggca tcgagattcc gtatccgcag
    26461 cgggtgctgc ggcgccatca cacgccgacc tgagccgacc gaaccggcca ccccgcggga
    26521 cgctcgggcg gtaaacagcc tggtcagccg cgtcagccga gcaatgcgac ttcctcgtcc
    26581 gtgaagcctg ccgcgcgtcg ggcggcatgg ttgaacggcg ggcgcagtcg aggcgcctga
    26641 tggcgttcgg ccagcacgtc gtaatgtgtc agcgggtcga ggccccgctg ggtgcagagc
    26701 cagcggtacc cgcggttgcc gatggcgacg tgaccgatct cgtcgcgcag gatgacgtcg
    26761 aggatctcca ccgcccgagc atcaccggca cgcctgaact tggcctgcag cggtggggtc
    26821 gcgtcgagcc cgcgggcctc cagggttcgc ggcaccagtg ccatgcgcgc aagcacgtcg
    26881 tcggcggtac gctgggtcat cacccacaag cctgcgtgtg catcgaagtc gccgtagtcg
    26941 aacccgaggc cacgcaggtg ctctctcagt aggctgaagt gcagcgcttc ctcgttggcc
    27001 acctgcagcc aatcgaggta gaaccgagcc ggcatcgcgt cgaagcgcca gaccgcatcg
    27061 agcgcgaggt tgatcgcgtt gaactcgatg tgcgcgatcg agtgcagcag ggctgcccgt
    27121 ccctcgatcg tcgagggcga gcgcgagggc acgttcctgg ggtccacgag gcagggacgc
    27181 gtcggacggc cgggtgcctc ctgaggcccg ggcgttccag cagccccgaa ttctgccgat
    27241 ggcgattcca gcgcatgccg atcgcgctcg gcccagagcg ttcgtgtctg ctcggccttg
    27301 gtgtcgggat cggattccat ccacacagcg agcgcgcggc ctcgcaagga aactgcagca
    27361 gcggattcgg aatgggcggg gtgcatctct agaattatcg acttcacctc aggagcgttt
    27421 cagtggctgt ttaccgtctg ggggacgccg tgccctcgat tgcgcccagt gcatgggtgg
    27481 ctgacagcgc gcaggtcatc ggcgatgtcg tgctgtccga aagcgccagt gtctggttcg
    27541 gtgccgtgct gcgggccgac aacacgcgct tgctgatcgg cgcgcgcagc aatgtgcagg
    27601 atggcgctgt catccattcg gacaccggtt ggccggccac gctgggctgt gacgtgacgg
    27661 tcggccacca ggcgatgctg cacggctgca cgatcggcga tggctcgctg atcggcatcc
    27721 aggcggtcgt gctgaacggc gcacgcatcg gcaagtcgtg cctggttgcg gcaggtgcgg
    27781 tggtcaccga aggcaaggag ttcgaggacg gctggctgat catgggcgcc cctgccaagg
    27841 ccgtgcgtcc gctcacgccc gagcagcttg ccggtctggc gcgcagcgct tcgggctacg
    27901 tcgcgaatgc acagcggttt cgcagccagc tggcccggat cgatacccag gcaccgcatg
    27961 agtgagctgc acaagttcat cttcgaaggc atgccggtgc gcgggatgct ggtgcggctg
    28021 accgagggct ggcgcgaggt cctgcagcgc cgcgtgacct cccacgacga gtttcccgag
    28081 ccggttcggg tgttgatggg cgagatggcc gcggccggcg cactgatgca ggccggcatc
    28141 aagttcgacg gtgcgctggt gctgcaggtg ttcggtgacg ggccggtcaa gttggccgtc
    28201 gtcgaggtcc agagcgacct cgcatttcgc gtgaccgcga aagtggtcgg agacgtggcc
    28261 gccgatgccc aggtggaggc aatgctcaac gttcacggca agggccgttg cgcgatcact
    28321 ctcgattcga ggaccaagtt cccgggtcag cagccctacc agggtgtggt gtcgctgcac
    28381 ggtgatcagc gggaaccgct gcagaagatc agcgaggttc tcgaacacta catgctgcag
    28441 tccgagcaac tcgacacgcg cctgatcctg gcggccaacg acgatgtagc tgccggactg
    28501 ctgatccagc gtctgccggt cgagggggcg ggcaacctgg gtcggcgaaa cgaggacgag
    28561 atcggcgtga acgaggactt caatcgcctg tcgatgctgg cagccaccct gacgcgtgac
    28621 gaactgctga cgctcgacgc cgacaccgtg ctgcgtcgcc tgttctggga ggaaccgttg
    28681 aagcgtttcc cacccctggc cggcgaggat ggaccgaggt tcgcctgcac ctgctcccgc
    28741 gagcgggtgg ggcggatgct gatgggactg ggccggaccg aagtggacga catcatcgtc
    28801 gagcagggga gggtcgagat cgggtgcgac ttctgcggca tcaaatacca cttcgacgcg
    28861 gttgatgtgg gcgagctgtt cacgccgacg ccgcagcagg cgcccggctc gacgacgctc
    28921 aactgagcgc ccggcccgct gatcccttcg gcggggcgcc acattcctga gcacagcgaa
    28981 gcatggccgc caggcttcga tcaacgtcgt cgtctgtcgt ggcccacgag cacacgctga
    29041 tgcgcatcgc ggttcggccc tgccagtacg tgatgccgca ccagcaggtg ccgtcgcgct
    29101 ggatcgcatc gatcgtgcgt cgcgtggtgt cggaatcgcc gaaacggacc agcacctgat
    29161 tcaacacgac gtcgttgagc acctcgtgcc ccgcgtcacc caggccccga gcaaagcgct
    29221 gcgcgtgccg gcagcatcgc tcgatcatct cgcgcaagcc gctgcgcccg agcgaggcca
    29281 gagcggccca ggcatcgatg ccgcgcgctc gccgcgacag ttccggcgtc agatcggagg
    29341 cggcggcgcc atcatcatca gcggcgagat agggagcggc gacgcccatc gtggagcgca
    29401 gcgcctcgcg atcgcgcacg aacaccagtc cgctgtcgta gggcacgttg agccacttgt
    29461 gcgcgtccgt ggcccacgag tcggcttcgg tcacgccgtc gcaaagatgt cggtgttgac
    29521 gagtcgcttg cgcccacagc ccgaacgcgc cgtccacatg cacccaggcg ccgtcctggt
    29581 gcgccttctg gatgaggctg gaaaaaggat cgcaggcgcc ggtgttcacg ttgccggcct
    29641 gcaggcagac gatgctcggt cccgacaggc gaggcagctg gtctgcgtgc attcgacctt
    29701 gggcatccac cggaacgcgg atcgcgcgat cccgtccgag gccgagcacc cccagcacct
    29761 tggtcacgct gggatgcgcc tcgtcaccga tcaccaccgt gatcggaggc gcgccggaca
    29821 atccctgcgc gtcgacatcc cagcccgctt gcgccagcac ggcgtggcgc gcggcggcaa
    29881 gaccgcagag gtttccagtc gtggcgccgc tgacgaacgc gccggcaacc gtggagggca
    29941 ggccgagcag atcgatgatc cagcgcaggg ccgtgtgctc gaacgcggcg gcgccgggcg
    30001 ccgcggccgg atacggagcg ttctgatccc aggccgtcgc cagccagtgt gctgccatgc
    30061 tgaccggcag cgtgccgccc accacatagc cgaagaagcg tccgccgttg ctggccaccg
    30121 tggccggtga ccccagttcg tcgagtcgac gcaagacgtc ctgaggccgt tgcggtgctt
    30181 ccgcgaggtc gcattcgatc tgcttcagcc ggctcaccgc gcccggcagc ggtgccacgc
    30241 tgcgttcatc cagccggtcg agatactgcg acgcccgccg cgcggcgtct ttgagcaacg
    30301 gatgcatcag accacctccg tgttgcgcac tgcaggtccg ttcgctgctc ggtccagcgg
    30361 gccggagcgt ggtctcacaa agcctatttc gccgccgcgc tccactgcac caggatctcg
    30421 tcgggcttga ccatgccgag ttcgaggcgg gctttctcct cgaccatctc caacccttcc
    30481 ttcaggtcgt tgacctcggc ggccagctgc tcgttgcgct gacgggctgt ttcgttgatg
    30541 ccgttctgct cttcgagccg gctgcgcagg tccagcacgt gcggcacgcc ggacttgccg
    30601 aaccagagtt cggcatgcac gagcccgatc agcgcgagga gtgcgaagct gatgaagcgc
    30661 acggcaggtt ccggtgcggg tgatgacgat tgacgatgtg cgaaccggag gtcagcgcag
    30721 gttgtagaac gcgctgcggc ccgggtagct ggcgacgtcg cccaggtcct cctcgatgcg
    30781 cagcagttgg ttgtacttgg ccatgcggtc gctgcggctc atcgagccgg tcttgatctg
    30841 accggcgttg gtgccgaccg cgatgtcagc gatggtcgaa tcctcggtct cgcccgagcg
    30901 gtggctgatg acggcggtgt agttcgcgcg cttggccatt tcgatcgcgg caaaggtttc
    30961 ggtcagcgtg ccgatctggt tgatcttgat caggatcgag ttggcgatgc gtttctcgat
    31021 gccttccttc aggatcgcgg tgttggtgac gaacaggtcg tcgccgacga tctgcacctt
    31081 gctgccgagg cggtcggtca ggtgcttcca gccgtcccag tcgttctcgg ccatgccatc
    31141 ctcgatgctg atgatcgggt acttgtcgac ccaggtcgcg aggatgtcgg tccactcggg
    31201 tgcggagagc gtcaggttct cgctgccgag cacgtacttg ccgtccttgt agaactcgct
    31261 cgcagcgcaa tcgaggccga tcgcgatctg ctcgccggcg gtgtagccgg ccttgtcgat
    31321 tgcttcgagg atcagctgga tcgccgcctc gtgattggcc acgctgggag cgaagccgcc
    31381 ttcgtcaccg acggcggtgc tcatgttctt gccgtggatg atcttcttga gcgcgtggaa
    31441 cacctcggcg ccgtagcgca ccgcttcgcg gaaagtcggt gcaccgaccg ggatgatcat
    31501 caattcctgc aagtcgaggt tgttgttcgc gtgcgcgccg ccgttgatga cgttcatcat
    31561 cggcaccggc agctgcatgc cgcccgaccc gccgaagtag cggtacagcg gcaggccgct
    31621 ttcctcggcc gcagcgcggg ccacggccat gctgaccgcg aggatcgcgt tggcgcccag
    31681 gcggctcttg ttgtcggtgc cgtccagatc gatcagggtg cgatcgagga aagcctgctc
    31741 gctggcgtcg agcccgagca cggcttcgct gatctcggtg ttgacgtgtt cgacggcgcg
    31801 catcactccc ttgccgaggt agcgcttggg gtcgccgtcg cgcagctcgt tcgcttcgcg
    31861 cgaaccggtc gaggctcccg acggaaccgc ggcgcggccc atcacgcccg attcgagcag
    31921 cacgtcgcat tcgacagtcg ggttgccgcg gctgtccagg atctctctgc cgacgatgtc
    31981 aacaatcgca ctcatggttc ttcctcaata aggtcaatgc aaaaaatcgg aggttcgagg
    32041 ggctgccggg aagggcgccg cgctcagcag ctgaaatcgt tctccagcag cggctgctgc
    32101 ttgaccacac ggtccagcga caccagctgc tcaagcaatg cacgcatgtg cctcaacgga
    32161 accgcgttgg ggccgtcgct catcgcattc atcgggtccg ggtgggtctc catgaacagg
    32221 ccggccacgc cgaccgccac ggcagcgcgg gccagcaccg gcacgaactc gcgctgtccg
    32281 ccactgctgg tgccctggcc gcccggcaac tgcaccgagt gcgtcgcatc gaacaccacc
    32341 ggggcgcccg tctcgcgcat gaccgccagc gagcgcatgt cgctcaccag gttgttgtag
    32401 ccgaagctca caccgcgctc gcaggccatg aagttgtctt cgggcagccc catctcgcgg
    32461 gcggcggcgc gggccttgtc gatcacgttg cgcatgtcgt gcggcgcgag gaactggcct
    32521 ttcttgatgt tcaccggctt gcccgactgc gccaccgcgc ggatgaagtc ggtctggcgg
    32581 cacaggaagg ccggcgtctg taacacgtcg accacggcgg cgacggccgc gatctcgtcc
    32641 tccgcgtgga cgtcggtcag caccggcacc tgcagctcgc gcttgacctt gcccaggatc
    32701 tcgagtcctc gctccatccc cgggccgcga aaggtgttgc cgctggatcg gttggccttg
    32761 tcgtagctgc tcttgaagat gaaggggatg ccgagcttgg cagtcatttc cttcagcgcg
    32821 ccggccacgt ccatctgcag ctgttcggac tcgacgacac aggggccggc gatcaggaag
    32881 aacggctgcg tgagaccgac ttcgtatccg cacagcttca tgccacggct ttcatcgaag
    32941 cgcctgggct cttgcgatcg cgctggtgtg acagcgcggc ctggatgaac gagttgaaca
    33001 gcggatgccc gttccagggc gtggacttga actccgggtg gaactgcacg cccatgaacc
    33061 atgggtgcac gctgcgcggc agctcgacaa tctcggtcag cttctcgcgc tgcgtcagcg
    33121 ccgagatcac cagaccggct gcgctcagct gatcgaggta attgacgttc gcctcgtagc
    33181 ggtgccggtg gcgctcggtg accacgtcgc cgtagatctc gtgggcgagg gtgccgggtt
    33241 tgacgtccga gctctgcgcg cccagacgca tcgtgccgcc catgtccgaa ctggtgctgc
    33301 gcttctggat ggacccgtcg gcatcctgcc attcctcgat cagcgcgatg accggatggg
    33361 gcgtttcggg atcgaattcg gtgctgttgg cttgcgcgag gcctgcctgg tggcgggcga
    33421 actcgatggt ggcgacctgc atgccgaggc agatgcctag gtaagggagc ttgttctcgc
    33481 gtgcgaactt cgcggtcagg atcttgccct cgacgccgcg cttgccgaag ccgccgggca
    33541 ccagcaccgc gtcgtatccg gagagctgac tcacgttcga ttcgttcagc atttccgagt
    33601 cgacgtactc gatgttgacc ttcgcatggc tgtgcatgcc ggcgtggcgc agcgcctcgt
    33661 tgagcgattt gtacgagtcc gacaggtcgg tgtacttgcc acacatcgcg atggtcagtt
    33721 cgcgctcggg gtgctcgaac tcgcgcacca ggtcgtccca gcgcgtcagg ttggctggcg
    33781 gcgtgttgat gcgcagcttg tcgcagatca gcccgtcgag tccctgctcg tgcaggatgc
    33841 gcggcacctt gtagatggtg tcgacgtccc acatcgagat cacgccccaa gtcggcacgt
    33901 tggtgaacag cgagatcttc tcgcgctcct catcgggaat gcgtcgatcg gcgcggcaca
    33961 gcagcacgtc ggcctggatg ccgatctcgc gcagcttctg cgcggtgtgc tgggtcggct
    34021 tggtcttcaa ctcgcccgca gccgcgatcc acggcacgta ggtcaggtgc acgaaggcgc
    34081 tgttgttcgg gcccatgcgc aggctcatct ggcgcacggc ctccaggaag ggcagcgact
    34141 cgatgtcgcc gaccgtgccg ccgatctcga cgatggccac gtcgaccgcc tggtcggtac
    34201 cgacaccggc gccgcgcttg acgaattcct ggatctcgtt ggtgacgtgc ggaatcacct
    34261 gcaccgtctt gcccaggtag tcgccgcgcc gctccttctc gagcacgctc ttgtagatct
    34321 ggccggtggt gaagttgttg gccttcttca tccgggtggt gatgaagcgc tcgtagtggc
    34381 cgaggtcgag gtcggtttcc gcgccgtcgt cggtgacata aacctcgccg tgctggaacg
    34441 gggacatcgt gcccgggtcg acgttgaggt aggggtcgag cttgatgagc gtcaccttga
    34501 tgccacgcga ttcgaggatc gccgcgagtg acgccgccgc aatgcccttg cccagcgagg
    34561 acacgacacc gccggtgaca aagacgtact tggtcattgc atgcagcacc tggttttcag
    34621 gatgctgtgg tgggaaacga tgattatagg aggggggtca aacgatcttc tcgcccgggt
    34681 gcgtgatctt gcgcatcggc tcgtagcggt tcgagggcac gcaaagcggg tgtgccgccg
    34741 ttgcgcctgc tgtcgtgtcg ttcgaccagt gcggatcgtc gagcgattcg aagacctgac
    34801 gcagcttctc tccccacgaa ccccggatca tccggaaata ggggttgtgt tcgtcgatgc
    34861 agacgatctg gttgcactgc agggcgtcgt tctcgtagac cagcaggtcc agcggcaggc
    34921 cgaccgacag gttcgacttc agggtggaat ccatcgacac cagagcgcat ttcgatgctt
    34981 cgtcgagcgg tgttgccggc gtgatcatcc ggtcgagcac cggcttgccg tacttcgatt
    35041 cgccgacctg gaagtagcag gtctcgcggg tggcctcgat gaagttgccg gccgaataca
    35101 ccaggaacag tcgcatcgcc tcgcccttga tctggccgcc gaagatcatc gacgcgttga
    35161 agtcga
//
LOCUS       DAOORV010000019        33825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1494_length_33825_cov_8.064288, whole genome shotgun sequence.
ACCESSION   DAOORV010000019 DAOORV010000000
VERSION     DAOORV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33825
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1494_length_33825_cov_8.064288"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="PKV56_04400"
     CDS             complement(<1..1698)
                     /locus_tag="PKV56_04400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX67084.1"
                     /translation="MILNSSQPSEDDVTYQPRVLLVDDDEVNLLLTSIALRERGFVIT
                     EAASGERALELLAGWQPDVVVLDALMPGMDGFETCRQLRQTGACASLPVLMLTGLDDD
                     ASINRAYEAGATDFFVKSNQWSLLAGRLRYLLRSSRTRLELELSQSRLARAQDLARMG
                     SFDWRIGSPAPVFSAEALRVFQLEPGTVLPFRDLLRMMPKEERRGKRALLAETLQSRG
                     GLTRDIPLVLADGLVHIIHTEASPEYNELGQLVGYAGIVQDVTDRWNAEHKIRQLANF
                     DSLTGLPNRNQLIERTERALEAARRMGHQVGLLLIDLDRFKVINDTLGHGAGDELLIE
                     VARRLRSCVRHSDQVTDNLLDSMGTRAFRTLEAMCRLGGDEFVALLPEVIDEHDAEIV
                     AARVLEQMRAPIHVGGQECFVTASVGIALFPRDGINVADLIRNSDVAMFAVKAQGRNA
                     ATVYRPQLAGKGREKLELESALHKAIERNELVLHYQPKIDVRAARMVGVEALMRWQRG
                     NVLVPPGDFIPLAEESGLIRPMSEWAIREAARQARVWLDSFGFADSIAVNLPSRMFDR
                     "
     gene            complement(1695..2156)
                     /locus_tag="PKV56_04405"
     CDS             complement(1695..2156)
                     /locus_tag="PKV56_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOX67085.1"
                     /translation="MTSLPLRPCSAVAGPESSSGGSQASNPSLRGQLDGEAIRRLREL
                     DPSGESHLLTRVFNAFETSLNRLLPQLIQARDANDASGVRLVAHTLKSSSATVGALEL
                     SRLCVAVEVQAREMRLDEASEGIAQILLEVEAVRVSLGNLSRRSDTIAGAA"
     gene            2595..3944
                     /locus_tag="PKV56_04410"
     CDS             2595..3944
                     /locus_tag="PKV56_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67086.1"
                     /translation="MCLGSAPLAFWSGTVSAEGGTAASSMTSGLGFALGIGFVVLALL
                     GLVTLQRRAEKCREEAADLRRQQNQGAALARLLDVWQWRTNSQHRLVSLIPPAGTVTP
                     PSLAPLSSDPQPVWSLFNAANPKTGQTGATEHAALRQRLEAQQPAEDVLIALRASESG
                     VRLGLLRALPVYDEQGGFIGHVGTVREQPGACAAPQPAGRPATQAAIPATEGMLAMPS
                     LTPLAIPAEQDAEAFSYTVSHDLRAPIRVVEGFTKIVKEDYGRLLDRVGNDHLDRVLG
                     AAARMNSMIDALLALSQLSAKPVSRQPVNLSQLAGFVVDDLRRQWPQHAVKMHIDPGM
                     QVHGDPTLLRVVLENLLGNAWKYTGKCTEPEVWFERCTDSTTPSFTVRDNGAGFDMRY
                     ADRLFGVFQRLHSATEFQGTGVGLASVRRIVQKHGGEVWAESEVGQGASFHFSLGNP"
     gene            complement(3994..4629)
                     /locus_tag="PKV56_04415"
     CDS             complement(3994..4629)
                     /locus_tag="PKV56_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67087.1"
                     /translation="MSIVQEQRGHNGKILVVDDDRLVLATLAHGLTEAGYEVIDADNG
                     DDAILLAREHRPDLALLDIRMEGMSGFDVAAYLREYCKMPFMFLSAFSDDDTVRQVHE
                     LGAVDYLVKPLDIGHIVPAVQAVFARLRATSGAAVSGEAPKPQPSASLTDALKPVEAM
                     AVGVLMHRFSLTRAAALERLQRMSTSNGRTTADESERVLQAVELLSGSADH"
     gene            complement(4785..8645)
                     /locus_tag="PKV56_04420"
     CDS             complement(4785..8645)
                     /locus_tag="PKV56_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX67088.1"
                     /translation="MDGSANLQSLQQFFDHLSDGVLLFDRRALVIFANTAALRRLPGV
                     VDRSLAQWEPALGAPLVEWLRQAIAGSPGAMRMPGQAAARSAVAARRLASQAPPPALL
                     ADGQVADAAWQALGTGLYALRLHWRDGRARAAGDPGAGPSARDHPQRADHAQPPVAAL
                     PPTIAGPVIAELMQVLWNSPFPAMLQDSRMRIVDVNQAFVAFCGYPREQLVGSDPIAL
                     EPEEDRASSLELRERWQDPSRRDAEPALVSRRIVAADGRERWFRATRSALTDENGRVF
                     KMSVLQDSTAEHVARERADRSAREIDDWFDLSPIGMVLFDEAGLLVRTNPAFDALAGV
                     VPTELSEASPELRQLLGWTDQGPSPALEPSAHPVVAHGWLPQPQGGLRRLRSIVRCYT
                     TPGGARRHMAILEDRSAEEERDLAQMQIGALMDTAGVGLATFQETSGWVRQRQLPAAE
                     ERADEASVPTSAALQSISRDIVAEESMAEYDTLQQALRTAQRAEVRYAIRHPQLGLRW
                     LLTRVEPATLASGKRTTSVVTLDITEQHQTQQRSEQLLRELTTILESTSAGIAYIRGD
                     VLVRCNRRFEAMLGLPSVGIAGSGIQELFGSSPQGQRIADETLQALSAGLIFETEFSM
                     EPAAASAAGAPVNGSHTPGPTRWYALSVRRTGPVSEQIEAIAVLSDITRLKAQQVELE
                     QLARDRELMFSLSEVGIAFLREGVLQRVNDALAQLAGYPAAELQGLALSSLFADADEH
                     ERLSAQEQNALLQHGRWIGERQLHQRDGRVVWVQVSTRLVGDDPSGGVIASFVNVDAR
                     HRAEHAVALQANRTRAILDSVLVGIVTVGPRGIEWMNRSARRMFGADLGDFIDQPIST
                     VATPEPDHPFRRTQYLEDLIEGEAETFECRVKARDGREFWVVGNAVVTGRESRGRQLT
                     YALLDIEQRRRAEARIEEAQASLQRIIEAAPMAITVRDARTLRILQANVVAAHSIDMT
                     PEQIVGRSPEEMFAPDDAAQRRLDMESALRASVVTQREYRSTEQGEARVWDARYLPLA
                     APGQAPDQLLMVATDVTEQRAAEQAKFDAAIAQREMLVKEVHHRIKNNLQGVAGLLQQ
                     IGTRKPEMAEAMSEVVGQVQAIAQVYGLQVGAGGPLHVTGVVEAIAGSVQRTFGHPIR
                     FTVQGEHADQWVLPEAESIPIALTLNELLTNAIKHSLKQHREAKPARDSAVPAVECTL
                     FCEDTGVRIVIRNRASLPDGFNLSRVPGGVSGLGLVRALLPRRCAKLTIEPDTDGEDV
                     VATVSIGAPGVVFAGRLAADVPAR"
     gene            complement(8735..9940)
                     /locus_tag="PKV56_04425"
     CDS             complement(8735..9940)
                     /locus_tag="PKV56_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX67089.1"
                     /translation="MSDEILGQVALGYSAFIDRNRAVTATRLTVFPLKPDLVLDAAQL
                     LQAVGSVWPPEGGRVSLNVTSESLLQDLLRTTPSHNLMVEVPAFMAADPANTESIVAL
                     HAHGTTLMLKGRPLKELPREVLPCFKQAIIDLADDRRIGPAGTAPAGVTRSIPHVLSG
                     IHSVADMEGAFERGAIAVLGWPIGDEISERPKGSREKSAPIDLQTVVELMSQVDREEP
                     VEKLENTLKRDPSLGFKVLRYINSPAFGLSVEISSFSHAVMLLGYQRLKRWLALLLAT
                     ASKEPNMKAAMFASVRRGLLMEELARHIGDQEMRNELFICGVFSLLDRLFQQPLAELL
                     RTVPVPQRVHETLIDGTGPYAPYFRLVQAIEAESLYDFRECAEALMMDVREINRAELR
                     ALASAADLD"
     gene            complement(9993..11228)
                     /gene="flgL"
                     /locus_tag="PKV56_04430"
     CDS             complement(9993..11228)
                     /gene="flgL"
                     /locus_tag="PKV56_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOX67090.1"
                     /translation="MRISTANQYDATIDNIQRRQLGMSEAQTRLTSGKRVLRASDDPA
                     AAARAERALATELRSTTSQRSAEASRVLASQSESALGDAQELLQQAREAMVAAGNASY
                     TDAERGIQADKLKGLREALLAVANRSDGSGSFLFGGQGSNQPPFIDSPAPAGVQFSGV
                     AGQTMTDADTSLPLTFDGPGAWLTARTGNGVFLTRSAGNSVTGQPVTGAVIDSGSVND
                     PSALFPVADTGYRVKFTSATTYDIESFPLATPAVVTPVTNGTYMDGRSIDLNGMSFTI
                     TGTPATGDQFEMTPSTPSLSVFDVLDDAVANLRTTGRTGAQIAQATSDGLRDVDASMG
                     NLRTARSAAGEVLNRIDTAFERLATQKLNAQTERSNAEDLDLVEAISDFNNKQTGYDA
                     ALKSYSLVQKLSLFQYLNP"
     gene            complement(11266..13275)
                     /gene="flgK"
                     /locus_tag="PKV56_04435"
     CDS             complement(11266..13275)
                     /gene="flgK"
                     /locus_tag="PKV56_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOX67091.1"
                     /translation="MGTGALSSLGVRALAASYSQLQTTGHNISNANTPGYSRQEAQLQ
                     TAGGQFTGAGFFGRGVDVTTVARAHDDFLTREAALSKAQASFDTARSEQLQRLEKVFT
                     TGETGLGYTAAQFFSAFADIANKPQDESGRQVALSQAGQLASRFNAAAEQLDDMQAGI
                     SADLKASVAQVNVLSQRIATLNQRISDARGLGQPPNDLLDQRELAISELSEFVQVTTI
                     EAEDGSLSVFMGGGQAIVLGAQTTALAAMTDPYDVSRVAIGISGPGGDQRLPTSLLQG
                     GSISGLISFQSRDLTDARNLLGQLALAIGQQVNDQQARGLDLLNQPGAAMFRFGAASG
                     LPVLAGRASTANTGTGTVSMTLQSPATLDAAQVSSVQASDYELRADGLGGFQLFRLNG
                     GSVDPQYAPQAVANGDLVDGFLISIGGAAAAGDRFLLQPAASAARDIHVDISNPKLIA
                     AASPLSATLAVGNLGTGSVASLVADSATAGTNPNLPATLSFQATATPGQFAYTWQDGT
                     GTSAPVVWQPGQAIDYPGSTPTDGFTITITGVPAAGDSFSILRNTYPLADNRNANAML
                     SLRDAPLVGAIWSGASLQPGTDVTDAYANILGNIGVRVQSARAAADLSTAVADDAELS
                     RSSRSGVNLDEEAARLIQYQQSYQAAAKMLQVAQSIFETLLQTAR"
     gene            complement(13298..14242)
                     /gene="flgJ"
                     /locus_tag="PKV56_04440"
     CDS             complement(13298..14242)
                     /gene="flgJ"
                     /locus_tag="PKV56_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HOX67092.1"
                     /translation="MSLSSPSSLSSMLGGSASATGAGTASAAIDTRALAALRNSASRD
                     PKAAIRETAKQFETMFMQQLMKGMREAQAAMSSGLMENSATKLGTEMLDSEYAKQMSG
                     APGGLADVIARQLERQMVGSTPADTAGSASRVSPAPAAGVPQSSLGPTPGTRQVDFVQ
                     RHTDAARAAEAQTGIPAGYMVAQAAHESGWGRREIRNADGTSSFNVFGIKAGAGWSGP
                     VAEVTTTEYVNGAPRKMTAKFRSYESYEAAFNDYAQLMKDSPRYSQVMAQASTGTVSA
                     AGFAQGLQKAGYATDPAYADKLTRVINTTLRLQRVMTT"
     gene            complement(14242..15372)
                     /locus_tag="PKV56_04445"
     CDS             complement(14242..15372)
                     /locus_tag="PKV56_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOX67093.1"
                     /translation="MDRTERLVGRLLWLGIFAASAAVWWPAPAMAARIKEVASVQGVR
                     SNPLVGYGLVVGLDGTGDQTTSAPFTTQSINSLLQQLGVTVPSGTNMQLKNVAAVMVT
                     AQLPAFAQPGQTIDVNVSSLANAKSLRGGMLIATPLKGADGQIYALAQGSLIVGGAGA
                     SAGGSKVQINHLSAGRVPEGATVERAVPTPLNQGDSLQLDLNASDYNTAREVARAINN
                     RMGGAIAQAVDGRRVHVRMPESPDARVSFMAEIENLNVDLASPAARVIINARTGSVVV
                     NEAVTLSACAVAHGNLSVTINSTPLVSQPNPLSQGQTVQAEKNDITIRQEPGSLIQLP
                     AGTKLADVVKALNSLGATPQDLLAILQAIKSAGALNAELEVI"
     gene            complement(15413..16141)
                     /locus_tag="PKV56_04450"
     CDS             complement(15413..16141)
                     /locus_tag="PKV56_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOX67094.1"
                     /translation="MNVLLATHFLQPSVLARRVLAGLGRTGLCLGIVALTGCEIMRPK
                     VDVAVPTSATPVAVAAPAVNNGAIFQAAQYRPLFEDHRARLVGDTLTVQIVEKVSATQ
                     KSTSTIDKTGKVSSSLTAAPFLPPRLFSRAGATGDSSNTFEGKGSTENTNDFSGVITA
                     TVTGVLPNGHLLVSGEKQIGVNENVDVLRFSGQVDPRSIQPGNSVASAQIANVRLEHR
                     GRGAQAEAQVIGWLARFFLSVLPI"
     gene            complement(16138..16920)
                     /gene="flgG"
                     /locus_tag="PKV56_04455"
     CDS             complement(16138..16920)
                     /gene="flgG"
                     /locus_tag="PKV56_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOX67095.1"
                     /translation="MMRSLWISKTGMEAQQTQLDTISHNLANVSTNGYKKSHAVFEDL
                     MYQNMRQAGANSTEQTTLPTGLQLGLGTRAVATSRSFAQGNLQQTTNPLDVAVRGNGF
                     FEVQMPDGTTGYTRDGSFQVSSTGQLVTNNGYTVSPGITIPTTAQSITIGSDGTVSVA
                     LPGQALPQTVGQLQLANFINPAGLEPKGQNLFAETASSGTATTGAPGANGLGALQQGF
                     VETSNVNVVEELVSMIQTQRAYELNSKAITTSDEMLQKLGQL"
     gene            complement(16981..17706)
                     /gene="flgF"
                     /locus_tag="PKV56_04460"
     CDS             complement(16981..17706)
                     /gene="flgF"
                     /locus_tag="PKV56_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOX67096.1"
                     /translation="MDRMIYLSMSGAKAMMQRQDTLAHNLANVSTPGFRAELQAFRAV
                     PVQGSGASTRVYALESTPGYDASPGPVTATGRNLDVAVKGNAWLAVQGLDGTEAYTRG
                     GSLELSADGTLTTRNGLTVLGDGGPIQVPQNSEVAIGEDGTISARAGNTPPTVVGRLK
                     LVTSEAPLTRGEDGLFRAADGDLPADATARVQDGALEGSNVSSVETMVAMISAARQFE
                     AQMKMMQTAKDDESAAAQLLSMS"
     gene            complement(17754..19025)
                     /gene="flgE"
                     /locus_tag="PKV56_04465"
     CDS             complement(17754..19025)
                     /gene="flgE"
                     /locus_tag="PKV56_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOX67097.1"
                     /translation="MSFQQGLSGLSATSKNLEVIGNNIANASTFGAKAARAEFGDMYA
                     TAIGGAGSTGAGIGVNLLTVAQQFTQGNITTTGNPMDLSINGRGFFQVASQSGETLYS
                     RNGQFKLDREGYVVNNTGDKLLAQGWDEAAGRAAGDAAPIRLQSGLGDPVATGAGSDP
                     ALRGVRLAINLDARKDVPTPAMSFTQPDSYNFSTSQTLYDSQGAPLTMNYYFRKTAAN
                     AWDVYASINGQAFDGPAPATGLVTAMTFDAEGKLTAPLVAPTIDIIDPRAAPLATPLF
                     AALPINLDGSSQYAAGFSISELKQDGFSSGELTGITFDGSGVVKASYSNGRSSNLAQL
                     QLVDFTNLQGLQPLGANLWKSTYASGDPTGGGAPGSSTYGALQAGALEESNVDLTGEL
                     VNLITAQRMYQANAQTISTQSEVLRTLTSLR"
     gene            complement(19075..19731)
                     /locus_tag="PKV56_04470"
     CDS             complement(19075..19731)
                     /locus_tag="PKV56_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOX67098.1"
                     /translation="MAIDFTTLNKTSSSGASGSSATAAADEAGSEDRFLKLLVTQLQN
                     QDPLKPMDNAQMTSQIAQINTVKGIEKLNTSVEGLSGQFAQMQALQGASLVGRDVVVP
                     GNVLDIADGVGQGGFELTGAVDAVQVEVLSPAGAVIDTFGLGALGSGMHSFDWPTTTA
                     TNDSGLRFRVTASNGTVKSTPTLLMRDRVNAVTTAGNTLNLELQNSGSVPYSSVKALN
                     "
     gene            complement(19761..20177)
                     /gene="flgC"
                     /locus_tag="PKV56_04475"
     CDS             complement(19761..20177)
                     /gene="flgC"
                     /locus_tag="PKV56_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOX67099.1"
                     /translation="MSMMNIFNVAGSAVSAQSQRLNVVASNLANADTVAGPDGSAYKA
                     RQVTFQTQLMGTMGGDAATAGVTVSNISEDQTPGRRVHDPKHPSADAEGYVTYSNVNA
                     VEEMVNMISASRSYQNNVEVMNTAKSLLLKTLQMGN"
     gene            complement(20260..20679)
                     /gene="flgB"
                     /locus_tag="PKV56_04480"
     CDS             complement(20260..20679)
                     /gene="flgB"
                     /locus_tag="PKV56_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOX67100.1"
                     /translation="MLDRLTSSIDFHGQALSLRAERQRLIASNIANADTPGYVSRDMN
                     FAQALKDATGSQALRVGTTGRLDTTQTGHIAGSGATPGQANLQYSSVSQTSLDNNSVD
                     MDRERASFVDNAVKYEATLRFINGNVRTMLDAIKGQS"
     gene            20831..21649
                     /gene="flgA"
                     /locus_tag="PKV56_04485"
     CDS             20831..21649
                     /gene="flgA"
                     /locus_tag="PKV56_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOX67101.1"
                     /translation="MKFQISRVSTSAARLAQVFISTSGLLLIGLLLILGTPLRAQAQA
                     AAVESIDTALVDQVRELALLATQQTAGLQSAGATNVDAGIGNPTRFDVVVGALDPRLK
                     LAPCQRIEPYLPTGVRLWGKARIGLRCAQGARPWNVYLPITVRVYGNALVAALPLQAG
                     ATVRETDLRLAEIDLAEQNAAVVASPALAVGRTLTRPLAAGQGLRQTDLKPRQWFAAG
                     DTVRVLATGPGFRVSGSGQALTPGLEGQTARVRTDGGQVITGMPVATRELEISL"
     gene            21788..22111
                     /gene="flgM"
                     /locus_tag="PKV56_04490"
     CDS             21788..22111
                     /gene="flgM"
                     /locus_tag="PKV56_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOX67102.1"
                     /translation="MKIGNPADKPAAPAAATTPAAPQPARTPAESGAAAQTTEASAQV
                     ALSSTATKLLSGASGVSADFDAEKVERISNAIEKGEYTINAEAIADKLIANAAEVLNN
                     TPSKT"
     gene            22138..22560
                     /locus_tag="PKV56_04495"
     CDS             22138..22560
                     /locus_tag="PKV56_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67103.1"
                     /translation="MNLSPSQNQLPAIPGTAAEIADPLLESTLSAVERRLSSLGEALR
                     TQDLSSIDLHATELHRALSNAVDHFSRAARNGPVPPALRRRLASAGGTVAAQRESLAR
                     ATAALDRAIDVLLPREAPVLYGSQGGAERLTRGGSLLA"
     gene            23184..24404
                     /gene="dinB"
                     /locus_tag="PKV56_04500"
     CDS             23184..24404
                     /gene="dinB"
                     /locus_tag="PKV56_04500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOX67104.1"
                     /translation="MSTQRPRLIAHLDMDAFYASVELLRYPDLRGQAVVIGGGRDRQP
                     VVQPDGTRRYATLRDYTGRGVITTCTYEARAFGVHSGMGVMKAARLAPEAVLLPVDFD
                     EYRRYSQRFKEAVRQVAPVIEDRGIDEIYIDLTDLPGAQDDAGRAAARALKDAVLDAT
                     GLRCSIGVTPNKLLSKIASELDKPDGLTLLDAADIPTRIWPLAAAKVNGIGPKAAAKL
                     AGFGLHTIGDVAAADPGFLLAEFGRSYGAWLHAAAHGRDDRPIVTHREPKSISCETTF
                     ERDLHAVRDRTALSECFSRLCERLAADLKRKGYATQTVGIKLRYDDFRIVTRDVTLPA
                     AIGDMAALRRAAGSCLKRVDLTRRLRLLGVRAGTLVRADELGASISAAAARPPPSTHQ
                     VDLPFIDESDEATA"
     gene            24526..25671
                     /locus_tag="PKV56_04505"
     CDS             24526..25671
                     /locus_tag="PKV56_04505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOX67105.1"
                     /translation="MPTITHIEDLRVLAKKRVPRMFYDYADSGSWTESTYRANEADFQ
                     RIQLRQRVAVNMQNRSTRVQMVGIDAAMPVAIAPTGLTGMQHADGEILAARAAEKFGV
                     PFTLSTMSICSIEDIAAHTTAPFWFQLYVMKDRGFIERLIERARAARCSALMLTLDLQ
                     ILGQRHKDIKNGLSAPPKPTIANLINLATKPRWCLGMLGTSRRSFGNIVGHAQGVGDL
                     SSLSSWTAEQFDPALSWADVEWIKKRWGGKLILKGIMDAEDARLAADSGADALIVSNH
                     GGRQLDGAPSSIAALPAIAEAVGSRIEVWMDGGIRSGQDVLKAWALGARGTLIGRSFL
                     YGLGAMGEAGVTRCLEIIRNELDLTMAFCGHTDIRTVDRKILLPGTF"
     gene            25818..26084
                     /locus_tag="PKV56_04510"
     CDS             25818..26084
                     /locus_tag="PKV56_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67106.1"
                     /translation="MTLDQFQSLRMWHLRHWQQHPVEKHTWEGVLTLWMSGWVGGPAA
                     LVLDLPWVWATSLALLCLPSLYVTMRERMHRNGRLRCDWITLVR"
     gene            complement(26098..26490)
                     /locus_tag="PKV56_04515"
     CDS             complement(26098..26490)
                     /locus_tag="PKV56_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67107.1"
                     /translation="MSRFLAVVLISLSLGFTSGCSTLVDAQAAKGTGTSRIYDKPYDT
                     VWSAVIETVKASDLALVAENKEKGTILAQGAISAFSWGENVAVFVEDAGGKARTRVEV
                     LNKRALAANITAKDWETRIFEALDRRLK"
     gene            complement(27090..27548)
                     /locus_tag="PKV56_04520"
     CDS             complement(27090..27548)
                     /locus_tag="PKV56_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HOX67108.1"
                     /translation="MSREGDLMRKALKRALLPELKLLGFSGTTSSFQRKSDKALDLLS
                     IQYWKYGGQFILEFARRERGDLHTSWGEVIPEERLDVAHTSPTQRARLQRKDDTSEEV
                     FRGFKFEGFGEDQTKFDALANQVASLLPQVIAWLETGVAGENVHAVGRGA"
     gene            complement(28092..28598)
                     /locus_tag="PKV56_04525"
     CDS             complement(28092..28598)
                     /locus_tag="PKV56_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67109.1"
                     /translation="MPMLSNRKRLLRTSELIALAGSAVMLLQAGKTLHSPPANFPAFL
                     AASVCLAGLLLTFLPVCMLGAEYIRTVRKATDPDERSDGLSSAEIAFLTRWSPRPYKA
                     AAWLALAILLGTLLFFGGVSITTREELDPAKVPAVFLYFSVFYLICLPVLGSATRMPG
                     TYASNSDA"
     gene            complement(28743..29051)
                     /locus_tag="PKV56_04530"
     CDS             complement(28743..29051)
                     /locus_tag="PKV56_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOX67110.1"
                     /translation="MARLINRMRAVHPGEVLREDYLVPLSLSVNALSIALGVPATRIH
                     EIVKERRAVTADTAARLAKYFGGDAASWLVMQANYDLKTLPTMGEIQRQVRVREHLAA
                     "
     gene            complement(29062..29340)
                     /locus_tag="PKV56_04535"
     CDS             complement(29062..29340)
                     /locus_tag="PKV56_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOX67111.1"
                     /translation="MIQSFKCKDTQALYEGKSPRRFRAFMAVAERKLAQLDAAQTLDF
                     LKAPPGNRLEALKGDRKGQQSIRINDQWRICFVWSNVGPVDVEIADYH"
     gene            complement(30643..31257)
                     /locus_tag="PKV56_04540"
     CDS             complement(30643..31257)
                     /locus_tag="PKV56_04540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX67112.1"
                     /translation="MNTDSSVSFFDQQFQRQVRESDLALNPFEQQALPHLQGVVLDYG
                     CGLGNLALAAARRGCNVVALDGSHTAIEHLRLVAQAESLSITANEADLRTYEVRGEYD
                     TVVCIGLLMFFDCPTAFAQLEQLQARVRPGGVAVVNVLVEGTTFHRMFDPSGHCLFAR
                     DEMNSRFVGWEILLSEYQDFAAPNNTTKSFVTIIARKPVVRVAA"
     gene            complement(31384..31833)
                     /locus_tag="PKV56_04545"
     CDS             complement(31384..31833)
                     /locus_tag="PKV56_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOX67113.1"
                     /translation="MLRQLLLLLHLLGAIAWLGGMFFAYFCLRPAAAEVLKPPERLPL
                     WSATFSRFLPYMAGAVAVILATGLSMLLSVGFSAAPPGWHVMFALGVVMAAVFGYIYF
                     SLFPKLQAHCKAQAWPSAAEVLNRIRQLVALNLVLGFLVVLAAVSAR"
     gene            complement(32772..33533)
                     /locus_tag="PKV56_04550"
     CDS             complement(32772..33533)
                     /locus_tag="PKV56_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOX67114.1"
                     /translation="MTNQLLEQLLPASLICIVTLAFFALERLRPGRALPAVRGWYLRA
                     ALMNVMQLALVGAGGIIWNKYFRDNALFNLGHWEYPALEGAFYWFVGTFIFYWWHRLR
                     HANGFWLVFHQIHHSPSRIEVLTSFYKHPIEIAADSILTGFLIYCVFGGSALAGAWTS
                     FFGAAGEYFYHSNIRTPKWVGFIIQRPEHHSIHHQLDVHRYNFGDLTIWDRMFGTFKD
                     ADNFAERCGFPGRNEERLAEMLSFKDVYAPSRSEA"
     gene            complement(33623..>33825)
                     /locus_tag="PKV56_04555"
     CDS             complement(33623..>33825)
                     /locus_tag="PKV56_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67115.1"
                     /translation="PWLLLPESATFEHERYLRQVAVYRKEFPPVVPALEVTGTDETKV
                     EMRARAALIASTTEQIIRRFAQ"
BASE COUNT         5840 a        10944 c        11087 g         5954 t
ORIGIN      
        1 gcggtcgaac atgcggctgg gcaggttgac ggcgatcgaa tcggcgaaac cgaaactgtc
       61 gagccagacg cgggcctggc gcgcggcctc gcgaatcgcc cattcgctca tcggccggat
      121 cagaccgctt tcctcggcca gcgggatgaa gtcgcctgga ggcaccagca cgtttccgcg
      181 ctgccaacgc atcagggcct cgacgccgac catgcgcgcg gcacgcacgt cgatcttcgg
      241 ctggtaatgc agcaccagtt cattgcgctc gatcgccttg tgcagtgcac tttcgagctc
      301 gagtttctcg cggcccttgc cggcaagctg cggccggtag acggtggcag cgttgcggcc
      361 ctgggccttg accgcgaaca tcgccacgtc cgaattgcgg atcaggtcgg caacgttgat
      421 gccgtcgcgc gggaacagcg caatgccgac cgacgcggtg acgaagcact cctgtccacc
      481 aacatggatc ggtgcgcgca tctgttcgag cacccgagcc gcgacgatct cggcgtcgtg
      541 ctcgtcgatc acttccggca gcagcgcgac gaactcgtcg ccaccgaggc gacacatggc
      601 ctccagcgtg cggaaggcac gggtgcccat cgagtcgagc aggttgtcgg tgacctggtc
      661 ggaatgccgc acgcacgaac gcagacgacg cgcaacctcg atcagcaact cgtcgccagc
      721 gccgtgcccg agggtgtcgt tgatgacctt gaatcggtca aggtcgatca gcagaagtcc
      781 gacctgatgc cccatgcggc gggccgcttc gagggcacgc tcggtgcgtt cgatcaactg
      841 attgcgattg ggcaggccgg tcagcgaatc gaagttggcc agctggcgga tcttgtgctc
      901 ggcgttccac cggtccgtca cgtcctgcac gatgccggca tagccgacca actgaccgag
      961 ttcgttgtac tcgggcgagg cctcggtgtg gatgatgtgc accagcccat cggccagcac
     1021 gagtggaata tcgcgggtca gcccccccct gctttgcagc gtttccgcca gaagcgcgcg
     1081 cttaccgcgt cgctcttcct tgggcatcat gcgcagcaga tctcgaaacg gcaggaccgt
     1141 gcccggctcc agctggaaca cgcgcagggc ctcggcagaa aacacgggtg ccggggatcc
     1201 aatgcgccaa tcgaagctgc ccatgcgggc cagatcctgc gcacgggcca ggcggctctg
     1261 gctcaactcg agttcgagcc gggtgcgcga cgacctgagc aggtagcgca ggcgcccggc
     1321 cagcagactc cactggttgg acttgacgaa gaagtcggtg gcgccggctt cataggcccg
     1381 gttgatcgag gcgtcgtcgt cgagtccagt cagcatcagg accggcagcg aagcacaggc
     1441 tcctgtctga cgcagctgtc ggcaggtctc gaaaccgtcc atgcccggca tcagggcatc
     1501 gagcaccacg acatcgggct gccagccagc gaggagttcc agcgcacgct cgccactcgc
     1561 cgcctcggtg atcacgaaac cgcgttcgcg cagggcgatc gaggtgagca ggaggttgac
     1621 ttcgtcgtcg tcgaccagca gcacacgcgg ctggtacgtg acgtcgtcct cggagggctg
     1681 gctggaattg aggatcatgc ggcacccgcg atggtgtccg agcgccgtga cagattgccg
     1741 agcgacacac gcacggcctc gacttccagc aagatctgtg caatcccctc gctggcttcg
     1801 tcaagcctca tttcacgagc ctgcacttcc accgccacgc acaggcgcga caactcaagt
     1861 gcaccgaccg tggccgaaga cgacttcagc gtgtgcgcca cgagacgcac accggaggcg
     1921 tcgttggcgt cccgcgcctg gatcaactgg ggcagcaacc ggttcagcga agtctcgaag
     1981 gcgttgaaca cgcgcgtcag caagtggctc tcgccgctgg ggtcgagttc acgcaagcgg
     2041 cggatggcct caccgtcgag ctggccacgc aaggacggat tcgatgcctg agagccgccg
     2101 gaggacgatt ccgggccggc aacggcggaa caaggtctca agggcaggga cgtcatgagc
     2161 agaggccagc aacgacagct ggggacagca gttcttcgcg catgccagag tgtgcctcag
     2221 acgcagggcc gagggcttcg tccaggcacg caacgccgtg gtcagccgaa aaattcgtgc
     2281 cgtgcggaaa tgaggccgga aggacgtacc gagagggttg catctggaat gagggggcgg
     2341 acaaaggtta ctgcagtggg atgaccgtga tcgtggtatc gaccgccttg ccctcaactt
     2401 gaatccaggt cgacccgcga caggctcgcg acggaaaccc gcacgcatcg gacactgcgc
     2461 cgaagcctgc cgggacgcac tggtgcccag atgggtgctg cctacaatct cccactatga
     2521 attgtggtct cggccagcac acgacagccg ataacagggc ggctgagccg ccagggcatc
     2581 ggtcgacgcg ggccatgtgc ctgggatcgg ccccgctggc attctggtct ggcacggtat
     2641 cggccgaggg cggcactgcc gcctcttcga tgaccagcgg tctgggtttc gcgctcggca
     2701 tcggattcgt cgtgctggcg ctgctgggac ttgtcacgct gcaacgccgc gcagagaagt
     2761 gccgcgagga agcggccgac ctgaggcgtc aacagaatca gggcgcggcg cttgcgcgcc
     2821 tgctcgatgt ctggcaatgg cgcaccaaca gtcagcaccg cctcgtgtcc ctcatcccgc
     2881 cggcaggcac ggtcacgccg ccctcgttgg ccccgctgag cagcgacccg cagcccgtgt
     2941 ggtcactgtt caacgcagcc aatccgaaga ctggacagac cggcgccacc gaacacgccg
     3001 ccttgcgaca gcgactggag gctcagcagc cggccgagga tgtgctgatc gcccttcgtg
     3061 cctccgaatc cggcgtgcga ctcgggttgc tgcgcgcgct gccggtgtac gacgagcagg
     3121 gcggcttcat cgggcatgtg ggcaccgtgc gcgagcaacc tggcgcctgc gctgcgccgc
     3181 aaccggcggg ccgacccgcc acgcaagcgg caatccctgc caccgaaggc atgctggcca
     3241 tgccatcgct gactccgctg gcgatacccg ccgaacagga tgcggaggcg ttcagctaca
     3301 cggtgtccca tgacctgcgg gccccgatcc gggtggtcga aggcttcacg aagatcgtca
     3361 aggaggacta cgggaggctg ctcgaccgcg tcggcaatga ccacctcgac cgggtgctgg
     3421 gcgccgccgc ccggatgaac agcatgatcg acgccttgct ggcgctgtcg cagttgtcgg
     3481 ccaagccggt atcgcgacag ccggtcaacc tctcgcaact cgccggcttc gtggtggacg
     3541 atctgcggcg gcaatggccg caacatgcgg tcaagatgca catcgacccg gggatgcagg
     3601 tccacggcga tccgaccctg ttgagggtgg tgctggaaaa cctgctcggc aacgcctgga
     3661 agtacaccgg caagtgcacc gaaccggagg tgtggttcga acgctgcacc gactccacca
     3721 cgccgagctt cacggtacgg gacaacggag ccgggttcga catgcgatac gccgaccgtt
     3781 tgttcggcgt gtttcagcgc ctgcacagcg ccaccgaatt ccagggcacc ggcgtgggtc
     3841 tggcctcagt acgccgcatc gtgcagaagc atggcggcga ggtgtgggcc gagtccgagg
     3901 tgggccaggg cgcgagcttc cacttctcgc tcggaaatcc gtagccccgg gaccagacgc
     3961 ggcctgcccg cgatggcacc gggatgcgcg ggctcagtgg tctgccgagc cggacagcag
     4021 ttcgacggcc tgaagcacgc gttccgattc gtcggccgtc gtgcgtccgt tcgacgtgga
     4081 catgcgctgc agccgttcga gggccgcggc tcgggtcagc gagaaccgat gcatcagcac
     4141 gccgaccgcc atcgcctcga ccggtttcaa agcgtcggtc aaggacgccg agggctgcgg
     4201 ctttggtgct tcgccggaca ccgcggcacc cgaagtcgcg cgaagccggg cgaacacggc
     4261 ctgaaccgcc ggaacgatgt gcccgatgtc cagcggcttc accaggtagt cgacggcacc
     4321 cagttcgtgc acctgccgca ccgtgtcatc gtccgaaaac gccgacagga acatgaaggg
     4381 catcttgcaa tactcgcgca ggtaggcagc gacgtcgaat ccgctcatgc cttccatgcg
     4441 gatgtcgagc aacgcgagat cgggacggtg ctcccgcgcc aacaggatgg cgtcgtcgcc
     4501 gttgtccgcg tcgatcacct cgtagcccgc ttcggtcagg ccatgcgcca gcgtggcgag
     4561 caccagacga tcgtcgtcca cgacaaggat cttgccgttg tgaccccgct gttcctggac
     4621 gattgacacc tgttgcttcc tgctgagccg gcttgcctgt aagagatctc ggtgaccaca
     4681 gcgccgcgat cggcaccttc gtcagaggtg tggattctgt ccgaaagccc ttccggtgaa
     4741 cagccagacg ccggcccgac ccctcctgca ggcggggatc gcggtcagcg tgccggcacg
     4801 tctgccgcga ggcgcccggc gaacacgacc ccaggcgcgc cgatcgacac ggtggcaacg
     4861 acgtcctctc catccgtgtc cggctcgatg gtcagcttcg cgcaacgcct gggcagcagg
     4921 gcccggacca gacccaggcc cgacacgcca cccggaacgc gcgacaggtt gaacccgtcg
     4981 ggcaggcttg cacggttgcg gatcacgatg cgcacgccgg tgtcctcgca gaacagagtg
     5041 cactcgaccg caggcaccgc gctgtcgcga gcgggtttcg cctcgcgatg ctgcttcagg
     5101 ctgtgcttga tcgcgttggt cagcaattca ttgagggtca gcgcgatcgg aatcgactcc
     5161 gcctcgggca gcacccattg gtccgcatgc tcgccctgca cggtgaagcg gatcgggtgc
     5221 ccgaacgtgc gctgcaccga gccggcgatc gcttcgacga caccggtgac atgcaacggt
     5281 ccgcccgcgc cgacctgcag cccgtagacc tgggcgatcg cctgcacctg cccgaccacc
     5341 tcggacatcg cctcggccat ctcgggcttg cgggtaccga tctgctgcag cagcccggcc
     5401 acgccctgca ggttgttctt gatgcggtga tgaacttcct tgaccagcat ctcgcgctgc
     5461 gcgatggccg catcgaactt ggcctgctcg gccgcgcgct gttccgtcac gtcggtggcc
     5521 accatcagca actggtcggg tgcctggccc ggcgcggcga gcggcaggta gcgggcatcc
     5581 cacacccgtg cctcgccctg ctcggtcgag cggtactcgc gttgcgtgac cacgctcgcg
     5641 cgcagcgcac tctccatgtc gaggcggcgc tgtgcggcgt catcgggtgc gaacatctcc
     5701 tcgggcgaac gcccgacgat ctgctctggc gtcatgtcga tgctgtgggc cgcaaccacg
     5761 ttggcctgca ggatgcgcag cgtgcgcgca tcgcgcaccg tgatcgccat tggcgcggct
     5821 tcgatgatgc gctgcagcga ggcctgcgcc tcctcgatgc gggcctctgc ccggcggcgc
     5881 tgctcgatgt cgagcaaggc ataggtgagc tggcgtccgc gcgattcgcg accggtgacg
     5941 accgcgttgc cgaccaccca gaactcgcgc ccgtcgcgcg ccttgacgcg gcattcgaaa
     6001 gtctcggcct cgccctcgat caggtcctcg aggtactggg tgcgccggaa cggatgatcg
     6061 ggttcgggcg tcgccaccgt gctgatgggc tggtcgatga agtcgcccag atccgcgccg
     6121 aacatgcggc gggccgagcg gttcatccac tcgatgccgc gcgggccgac cgtgacgatg
     6181 ccgaccagca ccgaatccag gatggctcgt gtgcggttcg cctgcaacgc cacggcgtgc
     6241 tcggcgcgat gccgcgcatc gacgttgacg aacgacgcga tgacgccgcc cgacgggtcg
     6301 tctccgacca gccgcgtgct cacctgaacc cacacgaccc gtccgtcgcg ctggtgcagc
     6361 tgtcgctcgc cgatccagcg gccgtgctgg agcagcgcgt tctgctcctg tgccgagagg
     6421 cgttcgtgct catcggcatc agcgaacagc gaactcagcg ccagtccctg cagttcggca
     6481 gccggatacc ccgcaagctg cgcgagcgca tcgttgacac gctgcagcac accctcgcgg
     6541 aggaacgcga tgcccacctc cgacaggctg aacatcagtt cgcggtcacg cgcgagctgc
     6601 tccagctcga cctgttgagc cttcagccgc gtgatgtcgg acagcaccgc gatcgcctcg
     6661 atctgttccg acaccgggcc ggtgcgccgc accgacagcg cataccagcg ggtcggcccc
     6721 ggcgtgtggc tgccgttgac cggcgcaccg gctgccgagg cggcggcggg ctccatcgag
     6781 aactcggtct cgaagatcag gccggcggaa agcgcctgca gcgtctcgtc ggcaatgcgc
     6841 tgcccctgag gcgaactgcc gaacagttcc tggatgccgc tgccggcgat gccgaccgaa
     6901 ggcagaccga gcatcgcctc gaaccgacga ttgcagcgca ccagcacgtc gccgcggatg
     6961 taggcgatgc cggccgaggt gctctcgagg atggtagtga gctcgcgcag caattgctcg
     7021 ctgcgctgct gggtctggtg ctgctcggtg atgtccagcg tcaccaccga ggtggtgcgc
     7081 ttgccggaag ccagcgtggc gggttcgacc cgggtcagca gccagcgcag gccgagctgc
     7141 gggtggcgga tcgcgtagcg cacctcggca cgctgcgccg tacgcagggc ctgctgcagc
     7201 gtgtcgtatt cggccatcga ttcttcggcc acgatgtcgc gactgatcga ttgcagtgcc
     7261 gccgatgtcg gcaccgaggc ctcgtcggca cgttcctctg ccgccggcag ctgccgctgt
     7321 cgaacccagc cggaggtttc ctggaacgtg gcgagtccga cgccggcggt atccatcagc
     7381 gcgccgatct gcatctgggc caggtcgcgc tcttcctcgg cgctgcggtc ttcgagaatc
     7441 gccatgtggc gccgcgcacc acccggcgtg gtgtagcacc gcacgatcga gcgcaaccgc
     7501 cgcagcccgc cctgcggctg tggaagccag ccgtgggcaa ccacgggatg ggcagacggc
     7561 tccagcgcgg gcgacggccc ctgatctgtc catcccagca gctggcgaag ttccggcgac
     7621 gcctcggaca attccgtcgg caccacccca gccagtgcat cgaaggccgg gttggttcgc
     7681 accagcaggc ccgcctcgtc gaacagcacc atgccgatgg ggctcaggtc gaaccagtcg
     7741 tcgatctcgc gggccgagcg gtcagcccgc tcgcgagcaa cgtgctcggc ggtgctgtcc
     7801 tgcaggaccg acatcttgaa gacgcggccg ttctcgtccg tcagcgcgct gcgcgtcgcc
     7861 cggaaccagc gctcgcggcc gtcggctgca acgatgcgac ggctgaccag cgcgggttcg
     7921 gcatctcgcc tcgaagggtc ctgccagcgc tcgcgcaatt cgagactgct ggctcgatcc
     7981 tcctcgggtt caagggcaat cgggtcgctg ccgacgagtt gctcgcgcgg gtacccgcag
     8041 aacgccacga acgcctgatt cacatcgacg atgcgcatcc ggctgtcctg cagcatcgcg
     8101 gggaacggcg agttccagag cacctgcatc agctcggcga tcaccggacc ggcgatcgtc
     8161 ggcggtagcg cagcgaccgg cggctgcgcg tgatcggcgc gctgcggatg atcacgtgcc
     8221 gatggcccgg cgcccgggtc accggcggcg cgtgcgcggc cgtcgcgcca gtgcaagcgc
     8281 aacgcataca ggcccgtgcc cagggcctgc caggccgcgt cggccacctg cccgtccgcg
     8341 aggagggccg gcggcggcgc ctgcgaggcg agacgacgcg ccgccaccgc cgatcgagcc
     8401 gcggcctgac ccggcatgcg catcgcaccg ggcgacccgg cgatcgcctg gcgcagccat
     8461 tcgaccaggg gcgcacccaa ggcgggctcc cactgcgcga gcgatctgtc gacgacgccg
     8521 ggcagccggc gcagcgcggc ggtgttggca aagatcacca gggcacgccg gtcgaacagc
     8581 agcaccccgt cggagaggtg atcgaagaat tgttgcagcg actgcaggtt ggcgctgcca
     8641 tccatcgagg tcaccacgtc gcggtttccg gagtcatgtc ggcgaatcta tcgtgcgccg
     8701 gaagggccgg cgcaacagca tgcttcaagg catttcagtc gagatccgcc gccgatgcca
     8761 gtgcacgaag ttcggcacgg ttgatctcgc gcacatccat catcagggcc tccgcacact
     8821 cgcggaagtc gtacagcgac tcggcctcga tcgcctggac cagccggaag taaggcgcat
     8881 acggtccggt gccgtcgatc agtgtctcgt gcacccgctg gggcacgggc accgttcgca
     8941 gcagttcggc cagcggctgc tggaacaggc ggtccagcag cgagaagacg ccgcagatga
     9001 acaactcgtt gcgcatttcc tggtcgccga tgtggcgcgc cagttcctcc atcagcagcc
     9061 cgcgacgcac ggaggcgaac atcgccgcct tcatgttcgg ttccttgctc gcggtggcca
     9121 acagcagcgc cagccagcgc ttgagccgct ggtatccgag cagcatcacc gcgtggctga
     9181 aggagctgat ctcgaccgac aagccgaagg ccggcgaatt gatgtatcga aggaccttga
     9241 agcccagcga cggatcccgc ttgagcgtgt tctccagctt ttccaccggc tcttcccggt
     9301 cgacctggct catcagttcg acgacggtct gcaagtcgat cggcgcagac ttctcgcgcg
     9361 aacccttcgg tcgctcgctg atttcatcgc caatcggcca gcccaggaca gcaatggctc
     9421 cgcgctcgaa tgcgccttcc atgtcggcca cggaatgaat gcccgaaagc acgtgcggga
     9481 tgctgcgcgt cacgcctgca ggtgcggttc cggccggccc gatgcggcga tcgtcggcca
     9541 gatcgatgat ggcctgcttg aagcagggca acacctcgcg cggcaactcc ttgagagggc
     9601 gtcccttgag catcagggtc gtgccgtggg cgtgcagcgc gacgatggat tcggtgttcg
     9661 ccgggtcggc tgccatgaac gccggcacct cgaccatcag gttgtgcgag ggagtcgtgc
     9721 gcagcagatc ctgaagcagg ctctcgctgg tgacattcaa ggacacccgc ccgccttcgg
     9781 ggggccacac cgaaccgacg gcttgcagca actgcgccgc gtctagcacc aggtcgggct
     9841 tcaacgggaa gacggtcagg cgcgtcgcgg tgactgcccg gttgcgatcg atgaacgccg
     9901 aatagcccag cgccacctga cccaggatct cgtccgacac aacaactcct acgcagtgga
     9961 acgcccggcc cgcgcagagc cggcggtggc ggtcacggat tgaggtactg gaacagcgac
    10021 agcttctgga ccagcgaata cgatttcagt gcagcatcgt agccggtctg cttgttgttg
    10081 aaatcggaga tcgcttcgac caggtccagg tcctcggcat tcgaccgctc ggtctgggca
    10141 ttgagcttct gcgttgccaa ccgctcgaac gcggtgtcga tgcggttcag cacttcgccg
    10201 gcggccgatc tcgccgtgcg caggttgccc atcgaggcat cgacgtcgcg caggccgtcg
    10261 ctggtggcct gagcgatctg cgcaccggtg cgcccggtcg tcctcaggtt ggcgaccgcg
    10321 tcgtcgagca catcgaacac gctcagcgac ggcgtcgatg gcgtcatctc gaactggtcg
    10381 cccgtggctg gcgtcccggt gatcgtgaag ctcatgccgt tgaggtcgat cgatcggccg
    10441 tccatgtagg tgccgttggt gacgggtgtc acgacggcgg gcgtggccag cgggaagctc
    10501 tcgatgtcgt acgtggtggc gctggtgaac ttgacgcggt aaccggtatc ggccaccggg
    10561 aacagcgccg acgggtcgtt cacgctgccg gaatcgatca cggcaccggt caccggctgg
    10621 ccggtgacgc tgttgccggc cgaacgggtc aggaacacgc cgttgccggt gcgcgcggtc
    10681 agccaggccc ccggcccgtc gaacgtgagc ggcaacgagg tgtccgcgtc ggtcatggtc
    10741 tgtccggcga cgccgctgaa ctgcacgccg gcaggtgccg gactgtcgat gaagggcggc
    10801 tgattggatc cctggccgcc gaagaggaag ctgcccgagc cgtccgagcg attggccacc
    10861 gccagcagcg cctcgcgcag gcccttgagc ttgtcggcct ggatcccgcg ttccgcgtcg
    10921 gtgtagctgg cgttgcctgc agcgaccatc gcctcgcggg cctgttgcag caattcctgc
    10981 gcatcgccca gcgcgctctc cgactggctg gcgagcacgc ggctggcctc ggcgctgcgc
    11041 tgcgatgtcg tgctccgcag ttcggtcgcc agcgcacgtt cggcacgcgc cgcggcggcc
    11101 gggtcgtcgc tggcccgcag gacccgcttg ccactggtga gccgcgtctg cgcctcgctc
    11161 atgccgagct ggcgacgctg gatgttgtcg atcgtcgcgt cgtactggtt ggcggtgctg
    11221 attcgcatgt cggtgtctca gtctgggaaa gcggcggatt ccggctcacc gggcggtctg
    11281 cagcagcgtc tcgaagatcg actgcgccac ctggagcatc ttggctgccg cctggtacga
    11341 ctgctggtac tggatcagtc gcgctgcctc ttcgtcgagg ttgacgccgg aacgactcga
    11401 gcggctgagt tcggcatcgt cagccaccgc ggtcgacaga tcggccgcgg ccctggcgct
    11461 ttgcacgcgc acgccgatgt tgccgaggat gttcgcgtac gcatcggtca cgtcggtgcc
    11521 gggttgcaga ctggcaccgc tccagatcgc gccgaccagc ggagcgtcgc gcagcgacag
    11581 catggcgttg gcgttcctgt tgtcggccag cgggtaggtg ttgcgcagga ttgaaaaact
    11641 gtctccggca gcgggcacgc cggtgatcgt gatcgtgaaa ccgtccgtcg gcgtgctgcc
    11701 ggggtaatcg atggcctggc ccggctgcca caccacgggg gcgctggtgc ctgtgccgtc
    11761 ctgccaggtg taggcgaact gacccggcgt cgcagtcgcc tggaagctca gtgtcgcggg
    11821 cagattgggg ttggttccgg ccgtggcgct gtcggcgacc agcgaagcca ccgatccggt
    11881 ccccaggttg cccaccgcca atgtcgcgct gagaggcgat gccgcagcga tgagcttcgg
    11941 gttgctgatg tcgacatgga tgtcgcgtgc cgcgctggcc gcgggctgca gcaggaagcg
    12001 gtcgcccgcg gctgcagcgc caccgatgct gatcaggaat ccgtcgacca gatctccgtt
    12061 ggcgaccgct tgcggcgcgt actgcggatc gacgctcccg ccgttgagac ggaaaagctg
    12121 gaagccgccc aggccgtcgg cacgcagttc gtaatcgctg gcctggacgc tgctgacctg
    12181 tgccgcatcg agcgtggccg gcgactgcag cgtcatcgag acggtgccgg ttccggtgtt
    12241 ggccgtggat gcccgacccg ccagcaccgg cagacccgag gccgcgccga agcggaacat
    12301 cgccgcgcca ggctggttga gcagatcgag acctcgggcc tgctgatcgt tgacctgctg
    12361 gccgatggcc aacgccagtt ggccgagcag gttgcgcgcg tcggtcagat ccctggactg
    12421 gaaggaaatc aaaccggaaa tcgagccccc ttgcagcagc gaggttggca gccgctggtc
    12481 gccacccgga ccgctgatgc cgatcgccac gcgagacacg tcgtacgggt cggtcatcgc
    12541 ggccagggct gtcgtctgcg caccgagcac gatggcctga ccgccgccca tgaagaccga
    12601 gagactgccg tcctccgcct cgatcgtggt cacctgcacg aactcgctga gctcgctgat
    12661 cgcgagttcg cgctggtcga gcaggtcgtt gggcggctgg ccgagcccgc gtgcatcgga
    12721 tatgcgctgg ttcagcgtcg cgatacgctg cgagagcacg ttgacctggg ccaccgaggc
    12781 cttcagatcc gcgctgatgc ccgcctgcat gtcgtcgagc tgctcggcgg ccgcattgaa
    12841 gcgcgacgcc agctggcccg cctgcgacag cgcgacctgg cgcccggatt cgtcctgcgg
    12901 cttgttcgcg atgtccgcga atgcactgaa gaactgtgcc gcggtgtatc ccaggccggt
    12961 ctccccggtg gtgaagacct tctccagccg ctgcaactgc tcgcttcgcg cggtgtcgaa
    13021 ggacgcctgc gccttcgaca gtgcggcctc gcgcgtcagg aaatcgtcgt gtgcccgcgc
    13081 gaccgtcgtc acgtcgacgc cacgtccgaa gaatccggcg ccggtgaact gcccgccggc
    13141 agtctgcaac tgggcctcct gccgcgaata tcccggcgtg ttggcgttgg atatgttgtg
    13201 gcccgtggtc tgcaactggg agtagcttgc ggcgagtgcc cgcacgccca gcgacgacag
    13261 tgctccggtt cccatggtgc tgcagtcctt cggatcttca ggtcgtcatc acacgctgca
    13321 ggcgcagcgt ggtgttgatg acgcgggtca acttgtcggc gtaggccgga tcggtggcgt
    13381 agcccgcctt ctgcaggccc tgcgcgaagc ccgcggcaga gaccgttccg gtgctggcct
    13441 gcgccatcac ctggctgtaa cgcgggctgt ccttcatcaa ctgggcgtag tcgttgaacg
    13501 ccgcctcgta cgactcgtac gagcggaatt tcgcggtcat cttgcggggt gcgccgttga
    13561 cgtattcggt cgtggtgact tcagccaccg gaccgctcca gccggcgccc gccttgatgc
    13621 cgaacacgtt gaacgaactc gtcccgtccg cgttgcggat ctcgcgcctg ccccagccgg
    13681 attcgtgagc cgcctgcgcc accatgtagc cggcgggtat gcccgtctgt gcctcggcag
    13741 cgcgcgcggc atcggtgtgt cgctgaacga agtcgacctg gcgggtgccg ggtgtcgggc
    13801 cgagcgatga ctgcggcaca ccggccgccg gcgccggcga gacgcgcgac gcggagcctg
    13861 ccgtgtcggc cggggtcgag ccgaccatct gccgctcgag ctgcctcgcg atcacgtcgg
    13921 ccaggccgcc aggcgcaccg ctcatctgct tggcgtattc ggagtcgagc atttccgtgc
    13981 cgagcttcgt ggccgagttc tccatcagcc ctgacgacat cgcagcctgc gcctcgcgca
    14041 tgcccttcat cagctgctgc atgaacatcg tctcgaattg cttcgcggtc tccctgatcg
    14101 cggccttcgg gtcgcgagaa gcggaattgc gcaacgccgc gagggcgcgc gtgtcgatcg
    14161 ccgcagaggc ggtgcctgcg ccagtcgcgc tcgccgatcc gccgagcatc gaggacaggg
    14221 acgatggcga ggaaagcgac atcagatcac ctccagttcg gcattcagcg cgccggccga
    14281 cttgatcgcc tgcaggatcg cgagcaggtc ctgaggcgtc gctcccagcg agttcagcgc
    14341 cttcaccacg tcagccagct tggtgccagc tggcaactgg atcagcgatc ccggctcctg
    14401 ccggatcgtg atgtcgttct tctcggcctg caccgtctgg ccctgcgaga gcggattggg
    14461 ctggctgacc agcggcgtgc tgttgatcgt gaccgacagg ttgccgtgcg cgaccgcgca
    14521 ggctgaaagc gtgaccgcct cgttcaccac caccgagccg gtgcgtgcat tgatgatgac
    14581 ccgggcagcc ggactggcca ggtcgacgtt gagattctcg atctccgcca tgaacgagac
    14641 ccgcgcatcc ggcgactcgg gcatgcgcac gtgcacgcgg cggccgtcga cagcctgggc
    14701 gatggcgccg cccatgcggt tgttgatggc gcgcgcgact tcgcgggcgg tgttgtagtc
    14761 gctggcattg agatcgagct gcaggctgtc accctggttc agaggggtcg gtacagcgcg
    14821 ctcgacggtg gcgccttcgg ggacgcggcc ggcgctgagg tggttgatct gcaccttgga
    14881 tccaccggcc gaagcgccgg cgccgccgac gatcaggctg ccctgcgcca gcgcatagat
    14941 ctggccgtcg gcgcccttca gcggggtcgc gatcagcatg ccgccgcgca ggctcttggc
    15001 gttggccagc gaggagacat tgacgtcgat cgtctgcccg ggctgcgcga acgccggcaa
    15061 ctgggcggtg accatgacgg ctgccacgtt cttcagctgc atgttggtgc ccgacggcac
    15121 ggtgacgccg agctgctgca gcagcgagtt gatgctctgc gtggtgaacg gtgccgaggt
    15181 cgtctggtcg ccggtcccgt cgaggccgac cacgaggccg tagccgacca gcgggttgct
    15241 gcgcaccccc tggaccgatg ccacttcctt gatgcgtgcg gccattgccg gtgcgggcca
    15301 ccacactgca gcgctggcgg caaatatgcc gagccagagc agcctgccta cgagtctttc
    15361 ggtcctgtcc atgtccggat gcaccccttg ttgcatccga ttctggacag acttaaatcg
    15421 gcagaacgct taaaaagaac cgtgccaacc agccaattac ctgagcttcg gcctgcgcac
    15481 cccgccccct gtgctccagg cgcacgttgg cgatctgtgc agacgccacc gaattgcccg
    15541 gctggatcga acgcggatcg acctggcccg agaagcgcag cacgtcgacg ttctcgttga
    15601 cgccgatctg cttctcgccg gagaccagca ggtggccgtt cggaagcacg ccggtcacgg
    15661 tggcggtgat caccccggag aaatcgttgg tgttctcggt gctgcccttg ccctcgaagg
    15721 tgttgctcga gtcgccggtg gccccggcgc gactgaacag gcgtggcggc aggaaaggcg
    15781 ctgcagtcag gctggagctc accttgccgg tcttgtcgat cgtgctggtg ctcttctgcg
    15841 tggccgagac cttctcgacg atctgcaccg tcagcgtgtc gcccaccagc cgggcgcggt
    15901 gatcctcgaa cagcggccgg tactgcgcag cctggaagat cgccccgttg ttgaccgccg
    15961 gtgccgcgac ggcgaccggt gtcgcactcg tcggcaccgc gacatcgacc ttcggccgca
    16021 tgatctcgca gccggtgagc gcgacgatgc cgaggcacag accggtgcgg ccgagaccgg
    16081 caagcacccg acgcgccagc accgatggct gcaggaaatg agttgcaagc aggacgttca
    16141 cagctgcccc agtttctgca gcatctcgtc cgaggtcgtg atcgccttgg agttcagctc
    16201 gtaggcgcgc tgggtctgga tcatcgaaac cagttcctcg accacgttga cgttggaggt
    16261 ctcgacaaag ccctgctgca gcgcaccgag cccgttggcg ccaggcgctc cggtggtcgc
    16321 cgtgcccgac gaggcggttt cggcgaacag gttctgtccc ttgggctcca gaccggccgg
    16381 attgatgaag ttggccagtt gcagctgacc caccgtctgc ggcagcgcct gtccgggcag
    16441 cgccacgctg accgttccgt cgctgccgat ggtgatgctc tgtgccgtcg tcgggatcgt
    16501 gatgcccgga ctgacggtgt atccgttgtt cgtcaccagc tgaccggtcg agctgacctg
    16561 gaacgagccg tcgcgggtgt agccggtggt gccgtcgggc atctgcacct cgaagaagcc
    16621 gttgccccgc accgccacgt ccagcggatt ggtggtctgc tgcaggttgc cctgcgcgaa
    16681 gctgcgcgag gtggccaccg cgcgggtgcc gagtccgagc tgcaagccgg tcggcagcgt
    16741 ggtctgttcg gtgctgttgg cgccggcctg gcgcatgttc tggtacatca ggtcctcgaa
    16801 caccgcatgc gacttcttgt agccgttggt cgagacgttg gcgaggttgt gcgagatggt
    16861 gtccagctgg gtttgctggg cttccatccc tgtcttggag atccacagag aacgcatcat
    16921 ggcgagggtc ctggttcagg cggtttcaaa ctgtcgggac gtcgtgcggg gtcgagccca
    16981 tcaactcatc gacagcaatt gcgccgcggc ggactcatcg tccttggcgg tctgcatcat
    17041 cttcatctgg gcttcgaact gacgggcggc gctgatcatg gccaccatgg tctcgaccga
    17101 gctgacgttc gagccttcca gcgccccgtc ctgcacgcgg gcagtcgcat cggcgggcag
    17161 gtcaccgtcg gcggcccgga acaagccgtc ctcgccgcgg gtgagcgggg cctcggaggt
    17221 caccagcttc aggcgaccga ccaccgtcgg cggcgtgttg cccgcgcgtg cgctgatcgt
    17281 gccgtcctcg ccgatggcga cttcgctgtt ctgcggaacc tggatcggac cgccatcacc
    17341 gagcacggtg aggccgttgc gggtggtcag cgtgccgtcg gcggacagtt cgagcgatcc
    17401 gccacgggtg taagcctcgg tgccgtcgag cccctgaacc gccagccagg cattgccctt
    17461 gacggccacg tcgagattgc gaccggtggc agtcaccgga cccggcgagg cgtcgtagcc
    17521 tggcgtcgat tcgagcgcgt agacgcgggt gctcgcaccg ctgccctgga ccggaaccgc
    17581 ccggaaagcc tgcagctcgg cgcggaagcc gggcgtcgac acgttcgcga ggttgtgcgc
    17641 cagcgtgtcc tggcgctgca tcatcgcctt cgcaccggac atcgacaggt agatcatgcg
    17701 atccatcggc tgctcctctc agacggggtt cagggtcagt ggctcggcaa cgatcagcgc
    17761 aggctggtca aggtccgcag gacctcgctc tgcgtcgaga tggtctgcgc gttggcctgg
    17821 tacatgcgct gcgccgtgat caggttgacc agctcgccgg tcaggtcgac gttggactcc
    17881 tccagcgcgc ccgcctgcaa ggctccgtag gtgctgctgc ccggtgcacc gccgccggtc
    17941 ggatcgccgg aggcataggt ggacttccac aggttggcgc ccaggggctg cagaccctgc
    18001 aggttggtga aatcgaccag ttgcagctgc gccaggttcg acgagcggcc gttggaatag
    18061 ctggccttga cgacgccgga gccatcgaag gtgatgcccg tcagttcgcc ggatgagaag
    18121 ccatcctgct tgagttcgga gatgctgaat cctgccgcgt actggctgct gccgtcgagg
    18181 ttgatcggca gcgcagcgaa cagcggcgtc gccaggggcg ccgcacgcgg gtcgatgatg
    18241 tcgatggtcg gggcaaccag cggtgccgtc agcttgcctt cggcgtcgaa ggtcatcgcc
    18301 gtcacgaggc cggttgcggg cgccgggccg tcgaaagcct gcccgttgat ggaggcgtag
    18361 acgtcccacg cattagcggc cgtcttgcgg aagtagtagt tcatcgtcag cggcgcgccc
    18421 tgactgtcgt aaagcgtctg cgacgtcgag aagttgtagc tgtccggctg cgtgaacgac
    18481 atggcgggcg tcggcacgtc cttgcgcgca tcgaggttga tcgcgagcct cacgccgcgc
    18541 agcgccggat cgctgccggc gccggtggcg accgggtcac ccagaccgct ctgcagccgg
    18601 atgggagccg catcgcctgc cgcccgaccc gccgcctcgt cccagccctg ggccagcagc
    18661 ttgtcgccgg tgttgttgac cacatagccc tcgcggtcga gcttgaactg cccgttgcgg
    18721 ctgtagaggg tctcgccgct ctggctggcc acctggaaga agcctcgccc gttgatcgac
    18781 agatccatcg ggttgcccgt cgtggtgatg ttgccctgcg tgaactgctg cgccacggtc
    18841 agcaggttga cgccgatgcc ggcgccggtg gagccggccc cgccgatcgc ggtggcatac
    18901 atgtcgccga actccgcacg cgccgccttg gctccaaagg tgctcgcgtt ggcaatgttg
    18961 ttgccgatga cctcgaggtt cttgctggtg gcgctcagac cggagagacc ttgctggaag
    19021 ctcatgctgt ttccttagag aagcgggggg atgttgggtc gggaaagaag tgactcagtt
    19081 cagcgccttg acgctgctgt agggcacgct gccggagttc tgcaactcga ggttcagcgt
    19141 gttgcctgcg gtggtgaccg cgttgacgcg gtcgcgcatg agcagggtgg gcgtcgactt
    19201 gacggtgccg ttggaagcgg tcacgcgaaa gcgcaggccg ctgtcgttcg tggcggtggt
    19261 cgtgggccag tcgaagctgt gcattccgct gccgagggcg cccagcccga aggtgtcgat
    19321 caccgcaccg gccggagaaa gcacctcgac ctgcaccgca tcgacagcgc cggtcagctc
    19381 gaagccgccc tgcccgacgc catcggcgat gtcgagcacg ttgcccggca cgacgacgtc
    19441 gcggccgacc agcgaagcgc cctgcagcgc ctgcatctgg gcgaactggc ccgacaggcc
    19501 ctcgaccgag gtgttgagct tctctatgcc cttgacggtg ttgatctggg cgatctggga
    19561 cgtcatctgc gcgttgtcca tcggcttgag cggatcctgg ttctgcagct gcgtgaccag
    19621 cagcttcagg aagcggtctt ccgatccggc ctcgtcggcg gcggcggtgg ccgacgagcc
    19681 ggacgcgccg ctgctcgacg tcttgttgag ggtcgtgaaa tcgatggcca tgggatggat
    19741 cctggtaggc ggcgaagtgc tcagttgccc atctgcaggg tcttgagcag gagtgacttg
    19801 gccgtattca tgacctcgac gttgttctgg tacgagcgcg aggccgagat catgttgacc
    19861 atctcctcga ccgcgttgac gttgctgtaa gtcacgtagc cctcggcatc ggccgacggg
    19921 tgcttggggt cgtggacacg gcggcccggc gtctggtctt cgctgatgtt gctgaccgtg
    19981 acaccggcgg tcgcggcatc gccgcccatc gtgcccatca gctgggtctg gaacgtcacc
    20041 tggcgcgcct tgtaggcact gccgtcggga ccggcgacgg tatcggcatt cgcgaggttc
    20101 gaagccacga cgttcagccg ctggctctgc gcactgaccg cactgcccgc gacgttgaag
    20161 atgttcatca tgctcatgac aagccctctt ccgacgagta cgtcaaccga tcccctgagg
    20221 ggaacccagc ccgcagttca accatcgacc ttcgattcat catgattgcc ccttgatcgc
    20281 gtcgagcatc gtgcggacgt tgccgttgat gaagcgcagc gtggcctcgt acttcaccgc
    20341 gttgtcgacg aagctggccc gctcgcggtc catgtcgacg ctgttgttgt cgaggctggt
    20401 ctggctgacg ctcgagtact gcaggttggc ctgacccggc gttgcaccgc tgccggcgat
    20461 gtgtccggtc tgggtggtgt cgagcctccc cgtggtaccg acgcgaagcg cctgcgagcc
    20521 ggtcgcatcc ttcagtgcct gcgcgaagtt catgtcgcgt gacacatagc ccggcgtgtc
    20581 ggcgttcgcg atattgctgg cgatcaggcg ctgccgctcg gcgcgcaggc tcaaggcctg
    20641 accgtggaag tcgatagaac tggtgaggcg gtcgagcatg tggcactccc gaaggtgggg
    20701 tgtgtgttcc ggcgaggtac gttgtgcggt cattgtcgag agcgacccgg ccgacataag
    20761 cgggaacaac agcctgaatg gccgtcaatt ccgccattgg ccggcacgct tgcgcgccta
    20821 gagtgcagcg atgaaattcc agatttcccg cgtctccacc tcggcagccc gcctggcgca
    20881 ggtgttcatc tcgacctccg ggctgctgct gatcggcctg ctgctgatcc tcggcacgcc
    20941 cctgcgcgcg caagcccagg cggcggccgt cgagtcgatc gataccgcgc tggtcgacca
    21001 ggtgcgcgag ctggccctgc tggcgacgca gcagaccgca ggcctgcagt ccgccggcgc
    21061 caccaacgtc gacgcaggca tcggcaaccc gactcgtttc gacgtggtcg tcggcgcgct
    21121 ggacccacgc ctgaagctgg caccctgcca gcgcatcgag ccgtacctgc ccaccggcgt
    21181 tcgcctgtgg ggcaaggcgc gcatcgggct gcgctgcgcc cagggcgcac ggccctggaa
    21241 cgtctatctg cccatcaccg tgcgggtgta cggcaacgca ctggtcgccg cccttcccct
    21301 gcaggccggc gccacggtgc gcgagacaga cctcagactg gccgaaatcg acctcgcgga
    21361 acagaacgct gccgtcgttg cttcgcctgc actggccgtc ggccgcacgc tcacgcggcc
    21421 gctggctgcg ggccaggggc tgcggcagac cgacctgaag ccgcggcaat ggtttgcggc
    21481 gggcgacact gtcagggtgc tggctaccgg acccgggttc agggtgtcag gcagcggcca
    21541 ggcactgacg cccggactgg aaggccagac cgcccgcgtg cgcaccgacg gcggtcaggt
    21601 catcaccggg atgcccgtgg ccacccgcga actggagatt tcgctgtgaa atcgttggct
    21661 cggtgcaaag cgagcgcggc tcccgctgcg acgggtcacc aaaaagacct aaagtcatgg
    21721 tcagcccggt cgaaataccg ttcatgttga aaaggccgct ccttcagccg cctgttggga
    21781 gaacttcatg aaaatcggaa accctgctga caagcctgcg gcacccgccg ctgctaccac
    21841 cccggcagct ccacagccgg cccgcacgcc ggctgaatcc ggcgctgctg cgcagaccac
    21901 cgaagccagc gcgcaggttg ccctgtcgag cacggccacc aagctcttgt ccggagcctc
    21961 cggcgtttca gccgatttcg acgccgaaaa ggtcgagcgc atctcgaacg ccatcgagaa
    22021 gggcgaatac acgatcaacg cggaggcgat cgccgacaag ctgatcgcca acgccgccga
    22081 agtgttgaac aacaccccat ccaagacctg acagtccacc cgagcgccgc ctacgccatg
    22141 aacctgtcgc cgtcgcagaa ccagttgccc gcgatccccg gtaccgccgc ggaaatcgct
    22201 gatccccttc tcgagtcgac gttgagcgcg gtcgagcgcc gcctgtcctc gctgggtgaa
    22261 gccctgcgca cccaggacct gtcctccatc gacctgcacg ccaccgagtt gcatcgtgcg
    22321 ctgtcgaatg ccgtcgatca tttctcgcgc gctgcgcgca acggcccggt gcctccggcc
    22381 ctgcggcgcc gcctggccag cgccggcggc acggtcgctg cccagcgcga aagcctggca
    22441 cgcgccacgg ctgcacttga tcgtgcgatc gatgtgcttc tgccccgtga agcgccggtg
    22501 ctgtacggct cgcaaggcgg tgccgagcgg ttgacacgcg gcgggtcgct gctggcctga
    22561 tcgttccctc gggtcttctg ccttcgtcgc aggcgcctga tcgctttctc cccttctgat
    22621 tggcttgctg gcagcccgcc gggtttcggc ccggctgcca agtaactttc ttctgctggc
    22681 acagaagaaa gtcaccaaag aagagtgccc gaacaccagc ccatttgagc tgctcgcttc
    22741 ggctacatac ctacttcggc ggctctggct cgagaacatc ctccgagcta ccgcctcggt
    22801 ttcctcgctc acattcagtc gactcggtat cgacacggct cgaccctgca ggtcatcgtc
    22861 gcgaggaccc gcagggtcga gccgaaacga gaacaagcgg ttgggcatta gcgcggtcac
    22921 cgtggccggc gtccggtgac cgttctcgtg ccagagcctc ggccgtcagc cttctggccg
    22981 cagcgtgaca gccaaattgg ctggtgttcg aggccctttg ctttggtgac tttcatttgg
    23041 gcccacaaat gaaagttact cggctgccgg gccgaaaccc ggcgggctgc accgtgcacg
    23101 cacaagagtt cgtcagggcg aatccccggc gcggtcttgc caatgccgct ccgccgcgcc
    23161 aagccaagcc caaaatgaca ggcatgtcga cacagcgacc ccgcctcatc gcccacctcg
    23221 acatggacgc cttctacgcg tccgtcgagt tgctgcgcta ccccgacctg cgcggtcagg
    23281 cggtggtgat cggcggcgga cgtgaccggc agccggtggt gcagcccgat ggcacccgcc
    23341 gctacgccac gctgcgcgac tacaccgggc gtggcgtgat caccacctgc acctacgaag
    23401 cccgcgcgtt cggcgtgcac tcgggcatgg gcgtgatgaa ggcggcccgg ctggcgccgg
    23461 aggccgtgct gctgccggtc gacttcgatg aataccgccg ctattcgcag cgtttcaagg
    23521 aggccgtgcg ccaggtcgcc ccggtcatcg aggaccgagg catcgacgag atctacatcg
    23581 acctcactga tctgcccgga gcacaggacg acgcaggtcg ggctgcggcc cgcgcgttga
    23641 aggatgctgt gctcgatgcc accggcctgc gctgctcgat cggcgtgacg ccgaacaagc
    23701 tgctgtcgaa gatcgcttcc gaactcgaca agcccgacgg cctgaccctg ctcgatgcgg
    23761 ccgacatccc gacgcgcatc tggccgctcg cggcagcgaa ggtcaacggc atcggaccga
    23821 aggcggcggc gaagctggcg gggttcggac tccacaccat cggcgacgtg gcggcggccg
    23881 acccgggctt cctgctggcc gagttcgggc gcagctacgg cgcctggctt catgcggccg
    23941 cacatggccg cgacgaccgg cccatcgtca cccaccgcga gccgaagtcg atcagctgcg
    24001 agaccacctt cgagcgcgac ctgcatgcgg tgcgcgaccg caccgcgctg tcggaatgct
    24061 tctcgcggct gtgcgagcgg cttgccgcgg acctgaagcg caagggctac gcgacccaga
    24121 ccgtcggcat caagctgcgc tacgacgatt tcaggatcgt gacgcgtgat gtgacgctcc
    24181 cggcggcgat cggcgacatg gccgcgctcc gccgcgccgc cgggtcgtgc ctgaagcgag
    24241 tcgacctgac tcgccggctg cggctgctcg gcgttcgcgc cggcacgctg gtccgggcgg
    24301 acgagctggg cgcctcgatc agcgccgccg cagcccggcc accgccatcg acccatcagg
    24361 tcgacctgcc cttcatcgat gaatcggacg aagcaacggc ctgaaaggtg acccggaccg
    24421 cttcggtctg gccaccggga ccgggcgcct gcgcgggcag tcaggacaaa gcgccgatgg
    24481 tgcgccgccg ccttagcatc cgcagatcgg actccggatg ccgcgatgcc cacgatcacc
    24541 cacatcgaag acctgcgcgt gctcgcgaag aagcgggtgc cccgcatgtt ctacgactac
    24601 gccgattcgg gctcgtggac cgagagcacc taccgcgcga acgaggccga cttccagcgc
    24661 atccagctgc gtcagcgcgt cgcggtcaac atgcagaacc ggagcacgcg ggtgcagatg
    24721 gtcggcatcg acgcggcgat gccggtggcc atcgcgccga ccggactcac cggcatgcag
    24781 catgccgacg gcgagatcct cgcggcgcgc gcagcggaga agttcggcgt cccgttcacg
    24841 ctgagcacga tgagcatctg ctcgatcgag gacatcgccg cgcacacgac cgcgccgttc
    24901 tggttccagc tctacgtgat gaaggaccgc ggcttcatcg aacggctcat cgagcgcgca
    24961 cgagccgcga ggtgcagcgc gctgatgctg acgctcgacc tgcagatcct cggccagcgc
    25021 cacaaggaca tcaagaacgg cctcagcgcg ccgccgaagc cgacgatcgc gaacctgatc
    25081 aacctcgcca ccaagccacg ctggtgcctc ggcatgctcg gcaccagccg gcgcagcttc
    25141 ggcaacatcg ttggtcacgc acagggcgta ggcgacctgt cgtcgctcag ttcatggacg
    25201 gccgaacagt tcgacccggc gctgagctgg gccgatgtcg agtggatcaa gaagcgctgg
    25261 ggcggcaagc tgatcctgaa gggcatcatg gatgcggaag acgcgcggct ggccgcggac
    25321 agcggtgcag atgcgctgat cgtgtcgaac cacggcgggc gccagctcga cggtgcaccg
    25381 tcgtcgatcg ccgccctgcc ggccatcgcc gaggccgtgg gctcgcgcat cgaggtgtgg
    25441 atggacggcg gcatccgcag cgggcaggac gtgctgaagg cctgggcgct cggtgcacgc
    25501 ggcacgctga tcggccgcag ctttctctac ggcctgggcg cgatgggcga ggccggcgtc
    25561 acccgctgcc tcgagatcat ccgcaacgaa ctcgacctga cgatggcctt ctgcggccac
    25621 accgacatcc gcaccgtcga ccggaagatc ctgctgcccg gcaccttctg aaccatcgag
    25681 acggccagcg gacagccgcg agcggcccgt gcgggagaac tgggcggtca ggaacgcgct
    25741 gatgcggctt ttgctgcggc gcacaagggc gtagattgaa gctgcagttt ccaccacccc
    25801 gttcgcagga gatcgcgatg acgctggatc agtttcagtc cctcaggatg tggcacctgc
    25861 gccattggca acagcatccg gtcgaaaagc acacctggga aggcgtgctg acgctgtgga
    25921 tgtcaggatg ggtcggcggg cccgctgcac tggtgctcga cctgccctgg gtctgggcca
    25981 cttcgctggc actgctctgc ctgccaagcc tctacgtgac gatgcgcgag cgcatgcacc
    26041 gcaacggccg cctgcgctgc gactggatca cgctggtccg ctgagctccg tcgggcgtca
    26101 cttcagccgc cggtcgagcg cctcgaagat tcgagtctcc caatccttgg ccgtgatatt
    26161 cgcggccagg gctcgcttgt tcagaacctc gactcgagtt ctcgctttgc cgcccgcatc
    26221 ttcgacgaag accgcgacgt tctcccccca gctgaaggct gaaatggcgc cttgggccag
    26281 gatggttccc ttctccttgt tctccgcgac cagagccagg tccgaagcct tgaccgtttc
    26341 gatcactgca ctccagacgg tgtcgtaagg cttgtcgtag atcctggatg ttccggtgcc
    26401 tttggctgcc tgagcatcca cgagcgtgga acaaccagaa gtgaacccaa gggacaggga
    26461 aatcaatacg accgcaagaa accgagacat gtcgcgctcc gtaaaaacat gggctgtagg
    26521 cttgtcgaac cgccccggat tttgagggga atctcgtctt caagcgggcg gagcctcctc
    26581 gaatcccgcg gcgggctcac ctggaatgga cctcgattcg aagtggagtt gctgcgacag
    26641 aacgccctgc ttcgcaccag acggctgtgc tcggcgtgaa tgtgtagatg ccttgtttcg
    26701 acagcacgac cgtgcgcgtg tggtcggacc ggaagtcggt gttgaatgtt gcgacgttcg
    26761 caggctcaac gatccagtgc aggttgtcgg cgacggctgc ttcccgttgg ggaggtatgg
    26821 ctgccatcca gcattggtca ggcttgagtt cccggtaggg gatgcgactc caacttccct
    26881 cggtcgttcg ctctgcgtag aggacgactg gcgtgccggt tccgacgctt cgggaagatg
    26941 ccgaggcggc aagttcaacc gtttgcggtg cctccaggac aaagacgaac tcaggctttg
    27001 ggtcgcagcc ctgcagggga atacatgcaa gagtgaagac cgcggcgaga ggcaaacgga
    27061 gcaaggtgcg aaccctctcg agtgaagcgt caggcacctc ggcccactgc gtggacgttt
    27121 tctccagcga ctccagtttc aagccaggca atgacctgcg gaagaaggga ggcaacttga
    27181 ttcgccagtg catcgaattt ggtctgatct tcgccgaagc cctcgaactt gaatccccga
    27241 aacacctctt ctgacgtgtc atccttgcgt tgcagcctgg cgcgctgggt cgggcttgta
    27301 tgcgcaacgt ccagtctttc ttctggaatg acctcacccc agctcgtatg aaggtcaccc
    27361 cgttcacgcc gggcgaattc cagaatgaac tgcccgccgt atttccagta ttgaatgctg
    27421 agcagatcaa gtgccttgtc agacttgcgc tgaaagcttg aagttgtgcc actgaagcca
    27481 agaagcttca actctggaag caaggcccgc ttgagcgcct tgcgcatcaa atcaccttcg
    27541 cgagacatat gagacgcctg acgttcgagc tcagccggca ccagcgggaa ggcgccaggc
    27601 ccgggctgtg gtgaatgtag cgcgccacaa gccgggccgg cctgcttgcc gttggggctc
    27661 tggggctcgg attcagtgag cggcaggcgt cattacggtg taccgcgctc ccaactgaac
    27721 gctccctgcc ttcggacact gcccaggtcc actcccaggc cagacagtgc ggcttcgaaa
    27781 tcttcccagt gaacgatgcc gtcgtcaccg acttcgagac tgagctcaac gaagccgggc
    27841 ttgtgcgccc aggaattgaa ctccgccagt cgtccgctac caaggatcag aaagaagtgg
    27901 cgcactgggc cggccccacc gccctttcga tccaggctga tccgagcttg atacctcgaa
    27961 gcgatggcct cgaagtcgca ggcgaaccac gcgaccggag tgatgtcgct tggcttgacc
    28021 cgggaaattg gtagccaggt gggactgatt gattccacca tgacgcctac cgttcgagtt
    28081 gaggcgagag gtcaggcgtc gctgttcgaa gcataggtac ctggcattcg agttgcagat
    28141 ccgagcacgg gaagacagat caagtagaag accgagaagt acaggaaaac tgccggtacc
    28201 tttgcaggat cgagctcctc gcgtgtcgtg atgctgacac caccgaagaa caagagcgtt
    28261 ccgagaagta ttgcaagtgc caaccatgcc gccgccttgt atggtcgagg agaccagcgg
    28321 gtgaggaacg cgatctcggc agacgacaac ccatccgatc tctcatcagg atccgtcgcc
    28381 ttgcgcacgg tacgaatgta ctcagcacct agcatgcaaa ccggcaggaa ggtgagcagg
    28441 agaccagcca ggcaaaccga tgcagccaga aaggccggga agttcgcagg cggcgaatgc
    28501 aaggtttttc cggcctgcag gagcatcaca gcactgcccg cgagcgcaat cagttccgat
    28561 gttcggagaa gtcgctttct gttgctgagc atgggcacgg gtgcgaggcc taacgattgc
    28621 gctgagggga acgcagcgga atggcactgt gtccgcgagg ctgatgattt gaacggcggg
    28681 ctaggcgggc ccagcgccat accggtgcgc ttcccctcga gcgaagggtt gcgcttcacc
    28741 tgctacgcgg ccaggtgctc acggacacga acttggcgct ggatctcacc catggttggc
    28801 aacgtcttca agtcgtagtt cgcctgcatg accaaccatg acgcggcatc gccaccaaag
    28861 tacttggcca agcgagcagc cgtatcggcc gtgacagcac ggcgttcctt gacgatctcg
    28921 tgaatacgag tggcgggtac accaagcgca atagagagtg cgttcacact caagctgaga
    28981 ggcaccaggt agtcttcacg aagaacctcc ccaggatgaa ctgcgcgcat gcgattgatt
    29041 aagcgtgcca tgtcgactcc ttcagtggta gtccgcgatt tcaacgtcaa cgggaccgac
    29101 attcgaccag acgaagcaga tgcgccactg gtcattgata cgaatacttt gttgaccctt
    29161 tcgatcccct ttcagggcct caaggcgatt gccaggaggt gccttcaaga agtcaagggt
    29221 ttgcgccgca tccagttgag caagtttgcg ttcggcaacc gccatgaacg ccctgaacct
    29281 cctcgggctc tttccctcgt acagggcctg ggtgtccttg cacttgaacg actggatcat
    29341 gccgcgagaa tattacgcga cacgtaatat ttcaagcagc tatgcggctc gctacagccg
    29401 ggggggcgtc acgcctaacg tttgagctga gcggcgggtt aagacgaact tgttggccac
    29461 tcgcacgatg cgtggacctt gcttcggcga taaaccttct cggaaccttg ggtagctacc
    29521 tggcgaagaa cctggatgtt cgggcccccg ctggggcggc cgcatgcacg aaactcttgt
    29581 gcatgcggcg gctggttgct acctgctact tgcccgtctc ggcaaacgcc aagcgcgccg
    29641 ctgagtgagc cttcaagcgc tcggatgcac ctggcacaga tcgcttgacg tctcgctttg
    29701 cgcggtgaac gttgtcgcgc aggacatgca acctcttgcg attgcgttgt gctttgatag
    29761 ccatgtattt ctccgttggt gaggacggag ggtactcctt acgcgatgcc aagccagtgc
    29821 gacacaggcg cctggttcac gggggctcag gggccgacct gtcgcttacg gaaaatccag
    29881 gagtgcctca tctctgggac tgttccgtgc gccctgccgt ttgagctgag gtgggaccga
    29941 cggtgtgacg ccaggtccgc cgttgggcct cagcttgagc gaggcgttag gcgtcatctc
    30001 tgtacgtcga ccttggcctc caaatgttgg cccattcggt acgctcgcac agatgcccgc
    30061 tgggccagag tattgaggcc ccttgacctc gatactgaac ctcctccggg ctcatgcctc
    30121 gagattccgc ccagttccgc caccacgtga ccatcattga ccaccagagc cacgccataa
    30181 gaaacccgac ggtcagcgcc aagaagtgag gtagcggagg ttcaaatgaa agccaagctt
    30241 gcaatgccaa gaggggcgtc agaagtagcg gcagggttgg caggctaacc aagcagactc
    30301 cgacagacac ggtaacgaat gctgactttc tggagacacc tgacgacact actcgcgccg
    30361 cgacggaaag tgcctcaagc gcctccctct gttcgacagg tagttgctcg agctgacgtg
    30421 ccaacttcct ctcctgccac cacccgaaga cagagagagc ggctagtgcc gcaagcatga
    30481 accccaagag agtgagcttg aagttccagt gcattgcatc tgcagtccac cgggcggcta
    30541 ggcccagccc aaggaagagt gccccgatag caccgtagaa cttgaagcgc aacatgggtg
    30601 ggttagatga cgcctaacgt tcgagccaag cggagcgagg ggttaagccg caacccgcac
    30661 cactggcttg cgcgcaatga tggtgacgaa ggacttggtg gtgttatttg gtgcggcaaa
    30721 gtcctggtac tcggaaagca ggatttccca cccgacaaag cggctgttca tctcgtccct
    30781 cgcaaagagg cagtggcctg acggatcaaa catcctgtgg aacgtagttc cttcaaccag
    30841 cacattgaca accgcaacgc cgccgggccg cacacgagcc tgcaactgct ccaactgggc
    30901 gaaggcggtg gggcaatcga agaacatcag caggccgatg cagacaacgg tgtcgtactc
    30961 gccgcgaact tcgtaggtgc gaaggtccgc ttcgttcgcg gtgatcgaca gggactcggc
    31021 ttgtgcaaca aggcgaaggt gttcgattgc ggtgtggctg ccgtcgaggg ccacaacgtt
    31081 gcagccccgg cgggctgcag ccaaagcgag attgcccaat ccgcacccgt agtcgaggac
    31141 gacaccttgc agatgcggaa gcgcctgctg ttcgaagggg ttcagcgcga gatcggactc
    31201 gcgtacctgg cgctggaact gctgatcgaa gaagctgacg cttgagtcgg tgttcatggt
    31261 gcccgcgagc aagggattac gaatttgcgg cctaacgttt gagctgaacc gcgagcgcca
    31321 tcccggtgga ggtccctggc tcgagcgaag ggttagcccg cacccttgca accgtgccgc
    31381 acgctagcga gccgacaccg ccgcaagcac gacgagaaag ccgagaacga gattgagcgc
    31441 cacaagttgg cgaatgcgat taagtacctc agctgcgctt ggccacgcct gcgctttgca
    31501 gtgcgcctgc agcttgggga agaggctgaa gtagatgtag ccgaagacgg ctgccatgac
    31561 cacgcctagc gcgaacatga cgtgccagcc tggcggtgcg gctgagaagc cgacagacag
    31621 aagcatggac aaccctgttg ccaggatgac cgctactgca ccggccatgt atgggagaaa
    31681 gcgagagaag gttgctgacc agagtggaag ccgctcgggc ggtttgagaa cctccgcggc
    31741 tgctggacgc aggcagaaat acgcgaagaa catgccgccg agccatgcga ttgcgccaag
    31801 aaggtgaagc aggagcaaga gttgtctgag catggccctg gcagtgcctt tggttgtgcg
    31861 ggctaacgtt ggagctgaag ggccgccgcc ggaaaggcgc ttagcccgcg agacggatga
    31921 tgacactcgc cagctcgcgg gccaagcggc ttgccggtgg agatccgctc gagcgaaggg
    31981 ttgggccgaa ccgtgcagcg tgcgaacgat gcgcgaggat ttctcacttg aaggggcgct
    32041 caggaccatg cccgagtact cgctccttcc accatgcata gccgttgggc aaccagaaca
    32101 gcgtcgctcg tgttgcaaac cgatgttgac ccagcgatct ggaaaagtcg tcgctgccga
    32161 acctgcggat gtacgaagca acgaggcgaa accgggtgcg tcgctgcatg gactttgcgg
    32221 ccgacggatg tagcagccgc tctcgctgcg ccgcgcctcg aactacacca cgaaaccgct
    32281 cctgtgacat gcgacccagc cagcgctgct gatgcagatt ttgctttgtg cgacgggcgt
    32341 agaacttaga gacgagcatt tcagttctgg aggcaaggca ctgcgctggc gtgcggtcta
    32401 acgtttgaat tcagcagctg ccgcaggcgg tctgctgcaa ctgatggcta gaggccatta
    32461 gtggacacga gcgtaaaaat tgccaaagta ccggcctgtc cttttactaa aaacgacagc
    32521 ggttccaact gtgagcgcct cgacggaatc aaacacttgc accgtcccgt cttccaaatc
    32581 tgctgtgcat cgaagtctgt tgttctgctt gctaatgctg acgcagactt gagcgattcg
    32641 gccctcgacg gtgacaccct caccaactgg tgtgttcaga gttgctgccg tcgcgcctag
    32701 agcgcagagt gcaatcgcca cgataagaat tggtcgcaaa gtgacgtcta gcgtttgagc
    32761 tgagcgaagg gttaggcctc gcttcgggac ggtgcataca cgtctttaaa gctgagcatt
    32821 tcagccaacc gttcctcgtt acgaccagga aagccgcatc gctccgcaaa gttgtctgca
    32881 tctttgaatg tgccgaacat tcgatcccag attgtgaggt cgccaaagtt gtacctgtgc
    32941 acgtccaact ggtgatggat ggagtgatgc tcgggacgtt ggatgatgaa gccaacccac
    33001 ttcggcgtac gaatgttcga gtgataaaag tactcgccgg cggcgccaaa gaatgaagtc
    33061 caagcgcccg caagcgccga accaccgaat acgcagtaga tgagaaaacc ggtgaggatc
    33121 gagtctgccg caatctcgat cgggtgcttg tagaaggaag taagtacctc aatgcggctg
    33181 ggactgtgat ggatctggtg aaacactagc cagaacccgt tcgcatggcg aaggcggtgc
    33241 caccagtaga agatgaacgt tccaacaaac cagtagaacg caccttctaa tgctgggtac
    33301 tcccaatgcc caagattgaa cagtgcgtta tcgcggaagt acttgttcca gatgatgcct
    33361 ccagcgccga cgagcgccaa ctgcatgacg ttcatcaatg ctgctcgtag gtaccagccg
    33421 cgtactgctg gcagagcgcg tcccggacgc aggcgctcaa gagcgaagaa ggcaagcgtg
    33481 acgatgcaga tgagagaggc agggagcagt tgctcaagaa gttggttagt cacgactgcg
    33541 tgttctgcga ggcctaacgt ttgatatgaa aggttggact cggctcgctg gtgcgagtcc
    33601 tctcgatgga agggttaggc atctactgcg cgaacctacg aatgatttgc tctgtggtgc
    33661 ttgcaatcag ggccgcacgc gcccgcattt cgactttcgt ttcatctgtc cccgtgactt
    33721 caagcgcagg gacgacagga ggaaactctt ttctgtacac cgcgacctga cgcaagtagc
    33781 gctcgtgctc aaaggttgca ctctcgggca ggagtaacca tggat
//
LOCUS       DAOORV010000020        33195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1536_length_33195_cov_6.183162, whole genome shotgun sequence.
ACCESSION   DAOORV010000020 DAOORV010000000
VERSION     DAOORV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1536_length_33195_cov_6.183162"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKV56_04560"
     CDS             <1..418
                     /locus_tag="PKV56_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOX67116.1"
                     /translation="IGIRSAGERAARDYVRDQGGLIHTARDLRGLESPTQLAGVFGGI
                     RSRLAQHGHPPVYLSLDIDCLDPAFAPGTGTPEPGGLTTNQVLSVLEELADLPFVGMD
                     CVEVAPAYDHAELTSNAAANFVWTYLCGRIASDPEA"
     gene            complement(476..958)
                     /locus_tag="PKV56_04565"
     CDS             complement(476..958)
                     /locus_tag="PKV56_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOX67117.1"
                     /translation="MRKQDPGAKLDAIDRALLEVLQVDAKLTNLELAERVHLSPSACL
                     RRVKALEESGVIAQYVALIDPKTVGRHGTSYTIVNLEKLTRSTMDTFEQAIRAVPEIH
                     DCFYVAGTNDYLLRFTYRDAEDLEHFHSHVLSSLPGVTRSNSMLVLRTVKKTTALPID
                     "
     gene            1106..2413
                     /locus_tag="PKV56_04570"
     CDS             1106..2413
                     /locus_tag="PKV56_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOX67118.1"
                     /translation="MQQNLDAYWMPFTANRQFKAAPRMISGASGMHFRTPEGREVLDG
                     IAGLWCVNAGHGRPRITEAIQKQAAELDFAPPFQMGHPKVFELAERLIELTPPGLDKV
                     FFTNSGSESVETALKMAIGYHRVRGEGQRTRLIGRERGYHGVNFGGISVGGMVANRKM
                     FGPMLGGVDHIRHTHDPVRNAFSKGQPEHGAELADDLERLIALHDASTIAAVIVEPVA
                     GSTGVLLPPKGYLERLRAICDRHGILLIFDEVITGFGRLGSPFAGNHFGVTPDLMTVA
                     KGLTNGCVPMGAVFAKRFIHDAFMNGPEHLIEFFHGYTYSGHPLACAASLGTLDTYAE
                     EGLLTRASRMAATFEAAVHSLAGLPHVIDIRNIGLVGGIELAPIAGDPGKRAFQVFLD
                     CWEQGVLVRTTGDTVALSPPLIIETGHIERIIDTLTGAIRRAA"
     gene            2468..3838
                     /locus_tag="PKV56_04575"
     CDS             2468..3838
                     /locus_tag="PKV56_04575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOX67119.1"
                     /translation="MSFLSKDLDLASNSYYDATVERTAARSALAGDIECDVAVVGGGL
                     AGLSAAIELADRGHDVVLLEARRVGWGASGRNGGQALAGLACDQDTIEAQLGLDEARR
                     VWAMTLEALALIRQRCERFHIDCDWRSGYLSLAVNARKARDLRAWHARMQSLYGFETR
                     WIEPAQLSSWIDSPRFHSGLHDPASGHLHPLKYSLGLARGAAELGVRLFENSPVTSVE
                     PAATAGSTVRVRTAAGSVRASHVLLAGNVYLQGVAPVLESRIMPVGTYIVASQRLDPA
                     LAQSLIPSGSAVCDTNFVLDYFRVASNPASTSPDTEPREHRMLYGGRVSYSTATPRDL
                     KSSMQRRMASTFPQLATTDVEFAWGGFVDISMNRAPDFGRLSDRDPRSGAGYANVYYV
                     QGFSGHGLALTGIAGRIVAEAIDGDTRRFDTFSRLRHHAFPGGKLLRTPALVAGMAYH
                     RLKDLL"
     gene            3915..4685
                     /locus_tag="PKV56_04580"
     CDS             3915..4685
                     /locus_tag="PKV56_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOX67120.1"
                     /translation="MTRKPFVLVPACHRMLGDHLNHTVGKKYTDAVRLAGCVPLLFTS
                     IQGPDELDDLLDAADGVLLTGSPSNVHPSHFGEIVYDPSLPLDPARDAATLAMIPAAI
                     QRGMPLMAICRGFQEVNVALGGSLYQAVHEVPGHLDHRGVAEGPPSEVYGLAHQVALQ
                     PGGLLEELLQCAAIEVNSVHGQGINRLAQALRVEARAPDDLIEAFTLANASSFLLGVQ
                     WHPEWEAHLNPVSTRLFEAFGRACCNFRDRHRGPDGIR"
     gene            4740..6107
                     /locus_tag="PKV56_04585"
     CDS             4740..6107
                     /locus_tag="PKV56_04585"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOX67121.1"
                     /translation="MPARENFTFSDLENWLNERRVTEIECLVPDLTGVARGKILPREK
                     FTEDRGMRLPEAIVAIGVTGEFPEEGPYYDVIHATDRDMHLRPDASTVRIVPWAADPT
                     AQVIHDCFDRDGKMIPFAPRSVLRHICDLYEAEGWAPVVAPELEFYLIERNADPNTPL
                     RPPKGRSGRAETSRQAYSIDAVNEFDPLFEDIYAYCEKMELNVDTLIHEVGAGQMEIN
                     FFHDHPLGLADEVFFFKRTIREAAMRHEMYATFMAKPMSNEPGSAMHVHQSVLNKVTG
                     KNIFSNPDGSPSKEFYWYIGGLQKYTPAAMALFAPYVNSYRRLARSTAAPINIQWGTD
                     NRTVGIRSPVATAAARRIENRVIGADANPYVAFAATLACGYLGIKNKIEPASECKGDA
                     YLSEYQLPRSLSEALGWLADEKDLHDVLGKSFITVYSEIKEIEHDEFMKVISPWEREH
                     LLLHV"
     gene            6158..7555
                     /locus_tag="PKV56_04590"
     CDS             6158..7555
                     /locus_tag="PKV56_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOX67122.1"
                     /translation="MNAAPDTTATRTTAEWQAADSAHYLHPFTDFKALASKGSRVITR
                     ADNIYLWDSDGAKILDAMSGLWCVNVGYGRDELIDAATAQLKQLPFYNSFFQTATPPA
                     IELAELLAEVTPPQFNHVFFSGSGSEGNDTVVRMVRRYWDVLGQPERSVIISRRNAYH
                     GSTVAGASLGGMSYMHAQGGLPIPGIVHMEQPFWFLHGHGKTRDAFGLEAASWLEAKI
                     LEVGPDKVAAFIGEPVQGAGGVIVPPATYWPEIQRICDKYGILLVSDEVICGFGRTGN
                     WWGCETMGFRPDLMTFAKGVTSGYIPLGGVMVGDRIAKVMIEQGGEFEHGYTYSGHPV
                     ACAVGLANIRLIQRDGLVEKVRNDTGPYLAEHFRSLAEHPLIGEAQSIGLMGALQIVR
                     DKASGACFDPALEVGMICRGHCFANGLIMRAVGDRMIIAPPLIITRAQIDEMARLIRL
                     CLDATLRDLQQRDLI"
     gene            7712..8812
                     /locus_tag="PKV56_04595"
     CDS             7712..8812
                     /locus_tag="PKV56_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX67123.1"
                     /translation="MALAAGAAAATAMCWAGPAVAQEEEKVLNIYNWSDYIAPDTIAN
                     FEKETGIKVRYDNFDNNEIVHAKLVAGKTGYDIVVPSSNWAKLQADGGLLRKLDKSLL
                     PNYKNLDPVVQAQIAKLDPGNEYMVDWLWGYTTVGINVDKVKAALGGLPMPENAWDLV
                     FKPEYVSKLKSCGVSYLDSATEVVPAALHYLGKPSFSKVAGDYAQAAAVLKAARPSVT
                     LFSSSGYINDMANGSICVALGWSGDINIARQRAIDGKTGQNIQALIPKSGGILFFDVM
                     VIPADAPHPGNAHKWINFILKPEVHASITNKVFYANPNAASRPLVKPEVANNPTVFLS
                     EADMKPMAAPDSLGNDLRRLMTRTFTAFKTGL"
     gene            8839..9972
                     /gene="potA"
                     /locus_tag="PKV56_04600"
     CDS             8839..9972
                     /gene="potA"
                     /locus_tag="PKV56_04600"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HOX67124.1"
                     /translation="MNIGSTPTSDSSKFLQIRNVTKDFSGYKAVNNVSLDISKGEIFA
                     LLGSSGCGKSTLLRMLARFETPTSGQILLNGQDLAGLPPYQCPMNMMFQSYALFPHMT
                     VWDNVAFGPRRSGMSKEEVAARVDEMLHLVQLGKFAQRKPHQLSGGQQQRVALARSLA
                     KKPQLLLLDEPLGALDKKLREETQIELVNIIENVGVTCVMVTHDQEEAMTMASRIAVM
                     SEGRFLQVGAPSDIYETPATRFVADFIGNVNLMEGTLTADHPDHVVIECADCKHYVGH
                     GITGSEGMAVTVALRPEKIRIGRQPLPPSDGEPAGGLNRVRGTVKDMGYFGSFTVYHL
                     QLASGLVMKISESNTERHREDELTWGDTAWASWSPLAHVVLTA"
     gene            10043..10966
                     /locus_tag="PKV56_04605"
     CDS             10043..10966
                     /locus_tag="PKV56_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX67125.1"
                     /translation="MKAMLSRLLSGRSAVIGIPYLWLLIFFLLPFLIVMKISVSEMET
                     VNFKDIITYTDDALALTLRFTTYLFITQDDLYYKTYLSSLKYAGVTTFFCLLIGYPFA
                     YFMARAKATVQPALLMLVMLPFWTSFLLRVYAWKGLLSENGWAYDVLVGSGLDQVLAA
                     LGLIPAPAQLMNSPFSLVLGMTYTYLPFMILPLYSNLSKMDMRLLEAADDLGATPFVA
                     FWKITVPLSKAGIIAGSMLVFIPCVGEFVIPELLGGPQTLMIGRVLWDEFFSNNDWPM
                     ASSVAVVMVLLIIVPLAIFNKYQAEATEVRK"
     gene            10963..11805
                     /locus_tag="PKV56_04610"
     CDS             10963..11805
                     /locus_tag="PKV56_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX67126.1"
                     /translation="MSGSLLNRWFGKGWLAFGFAFLYLPIVALVLFSFNDSPVPNAWR
                     GFTLKWYEALAADHEMIAAFWLSLKIAFFTACASVVLGTLAAFALVKYRRFTGRTLFS
                     GMVSAPLVMPEVIVGLSLLLMLVSMQRALGFPERGALTIWMGHLLLGMAYATVVIQAR
                     LQDLNPQLEEAAMDLGARPWEVFTLVTLPMITQSLMSAWLLTFTLSLDDVVLSAFLSG
                     PGSTTMPLVIFSRARLGLNPSVNAVATVIVVIVAIGVVAASYLIARAERNRSLDMAAA
                     NRAS"
     gene            11868..13259
                     /locus_tag="PKV56_04615"
     CDS             11868..13259
                     /locus_tag="PKV56_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3138 family protein"
                     /protein_id="HOX67127.1"
                     /translation="MKTWHCTVLTLAIAAAFPLQASAQSNEELLKELRALRDRVTQLE
                     EKLKSAAVPQAPAAAIPPAAVAPAGMTPDQAREMARLALKVDTLEEERENSNFKGLTI
                     SGSVDPTFIYNHAQNRAGFQLLNSATTGPYAYDNSFFGSASLDFQKELEGGTKFRVTL
                     QPDRGVGAHINAGSIVHEASVSIPIDGDETRLIVGQIPDWSGYELLASNENKLVTHNL
                     LFDLIAPLAYTGVGLEVTRGDWVIKGVVGNFNSTKREPDEKSPMLAYRGDYSISEYSG
                     IGFSGVHGKAANFVQETGDTTLHLFEVDGYYMRGDWTVQGQVSFGTQKDAAIALSSSD
                     RPKDAEWWGLSGLTAYKFTPRLEGVVRADFIYNRKNGGGLLGFGADSLNGIGPGFVGY
                     DAEGAAEARSSQHGSNRYALSVGGNYRFNEYTLFKAEYRFDGSTRRVFEYVDDGNFKK
                     TNHLFGASVVVSF"
     gene            13263..14762
                     /locus_tag="PKV56_04620"
     CDS             13263..14762
                     /locus_tag="PKV56_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOX67128.1"
                     /translation="MTTAATRDWHALAAALRPDGRVFIEGVRRAAAAEETFACISPID
                     GRTIAQVARGQAADIDAAVTSARRAFDDGRWAHRPPAARKKVLLRFAENILAAKEELA
                     LLETLDMGKPIQHSLGVDVPSSARCIAWYAEAVDKIYDEIAPTARNALALITREPMGV
                     VGAIVPWNYPMIMSAWKLGPALAAGNSVVLKPSEKSPLTAMRLAELAVEAGLPPGVFN
                     VVPGFGHEAGEALALHMEVDAIGFTGSTRVGRKMLEYAGRSNLKRVYNELGGKSAFIV
                     FDDFTDIPRAACTVAGSMFFNQGESCNAPSRVLVHESVADEFIAIVAAEAPTYQPGDP
                     LGGSAPMGALVDDGQLRTVLGYIDAGHTDGARLIAGGQQVRAETGGCYVEPTVFDGVT
                     NQMKIAREEIFGPVMSVIRFKTEVEAIAIANDSPYGLQASVWSHQIDRAHRVARSLRA
                     GTVHVNQYDEDDITVPFGGFKQSGNGRDKSLHAFDKYTELKTTWLRIDP"
     gene            14759..16051
                     /gene="gabT"
                     /locus_tag="PKV56_04625"
     CDS             14759..16051
                     /gene="gabT"
                     /locus_tag="PKV56_04625"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HOX67129.1"
                     /translation="MNLSPNEQLMARKAAATPRGVGVMGNFFADHAENAELWDIEGRR
                     YIDFAGGIAVMNTGHRHPRIVAAIEAQLKRFTHTCYQVVPYEGYIALAEKLNALTPGK
                     HAKKTALFSTGAEAVENAIKIARAHTRRAGVIAFSGAFHGRSLFAVALTGKVQPYKAQ
                     FGPFPPELYHVPFPCHCASLEEVKKAVQQLFKADIEPSRVAAIIFEPVQGEGGFNVIQ
                     PAAVQWIRELCNEHGIVMIADEVQTGFGRTGKLFAMQHFESLGVVPDILVSAKSLAAG
                     LPLSAVTGRADIMDAPNPGGLGGTYAGNPLAIAAAHAVIEVMADEGLPERGRQLGDRL
                     KAQLESMRAEVPQIADVRGLGAMVAVEFNRAGTAEPDADFTRRVQAAALARGLIVLTC
                     GVYGNVVRFMFPLTIPTAHFDEALGMLVGALRDAIQPA"
     gene            16056..17435
                     /gene="glnT"
                     /locus_tag="PKV56_04630"
     CDS             16056..17435
                     /gene="glnT"
                     /locus_tag="PKV56_04630"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HOX67130.1"
                     /translation="MKTSLPDIQPAAFQALRERLAADGVHTLMAQFTDLHGVARGKLV
                     PLNQLELLMTEGVGFAGPSIWGTGLPRIGPRAEYYGRGLIDSVRALPWMPGIARVVCD
                     GFVAGEPFEACPRQVLKRQMARLAERGWTLRTGIEPEFFLLRRSADGFEPADPHDRLD
                     KPSYDLKSLPRQREFLRQLADGLTRCGLDVFQIDHEDAHGQYELNFHHADALASADHL
                     MLFKLAAHALAEQHGMVFSMMPKPFANQPGSGMHFHVSLWNASGAGRTGLDARCVFDD
                     PAHDLSVTGRQFIAGVLHHADALCAIAAPTVNSYKRLTVGESITGTTWAPAYVAHGPN
                     NRTALVRTLKGRFEWRVPDASANPYLATAALIAAGLDGIDRKLDPGLPCTDDLFELSL
                     SQIHDRGIAVLPQSLSSAVDALERDEVIRSALGATLSSEFIRLKRAEWTEYARHVSPW
                     EMARYAAAF"
     gene            complement(17769..18815)
                     /locus_tag="PKV56_04635"
     CDS             complement(17769..18815)
                     /locus_tag="PKV56_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67131.1"
                     /translation="MFGVPIASKRAFENPLKRPGEIAPLITGVEAARLAARHTIGDKE
                     IRMAMLTCLTFARRFGAVATLLALLAACATPRIEPWSVSEGLNAPESAYLDRESGLLF
                     VSQVGLRPGGTPMDKDGNGVISKLTRDGKVLALNWVTGLNSPKGLRSHGGTLWVADVD
                     EMVSIDISSGTIKSRIKVDGAKFLNDVATGPDGAVYVSDMLTSKIHRIKDGIPTTFAE
                     GEQLEYPNGLLVEGDSLVVAAWGKPEANFSTKVPGHLYRLNLTTGAKTLITSKPTGNL
                     DGLESDGQGGYVVSDWVAGKILHVAANGDTRLLRQFKQGTADLAYLPESRTLILPHML
                     ENRVQAYDLSADFK"
     gene            complement(19418..21757)
                     /locus_tag="PKV56_04640"
     CDS             complement(19418..21757)
                     /locus_tag="PKV56_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOX67132.1"
                     /translation="MDKQPASSAAAPAWAAWPGLQYVVDAMERSVLFMDTLRERGNHY
                     VAHQDEGAPPLLKFKHELLIDGRKLSRPCNYALLHILPLDGVRAHPTARPLVVVDPRA
                     GHGPGIGGFKSDSEVGVALRAGHPVYFVTFHPQPEEGQTLFDVMNAEAMFLEEVIARH
                     PKASGKPVVIGNCQAGWAISMLASVRPELFGPLMIVGSPLSYWAGGSKLNPMRYSGAS
                     LGGAWLASMTSDLAGGRFDGAHLVENFEKLNPANTLWSRHFKLWANVDTESERFLEFE
                     RWWGGYFRMSGPEIETIVENLFVGNKLASGQVMTDERRVDLRNITAPVVVFASWGDNI
                     TPPPQALNWIIDAWGSERAIAAAGRVIVYVLHESVGHLGIFVGADIARKEHDQIVTSL
                     DVIDHLPPGLYEMKVVKKDGENVQRWDDLEPGSYSVCFEHRTMADIQAINPEGRDEEQ
                     LFSTVAKFSEINSSLYKTWVRPWLAALPLRPLGDAMTQMHPLRMQRQMFTDANPLAAP
                     VKELARKVRESRHALPDDHPGRVWEQALSKSMENSLNMYRDLRDQATVQWTRMVYGPM
                     GIGAWLPPDAPAEQLAAARAQAEIDELRRELLPHIGEGGFAQAVCRVVLAGMVSIGTF
                     ERRSFRLARLLSDLRVVEGAQGDGLGASIDWRRLLREEARIAAVAPVEALNALGDMLP
                     DESSRERALAVAAAVMMIEPTLANPRSEIIELLINTLGVEPERVMDLACRLTHPLGQL
                     AQPAPEVAKVKRERRPRVPAALVGKPRVRAAAPIRGLRT"
     gene            21923..22789
                     /locus_tag="PKV56_04645"
     CDS             21923..22789
                     /locus_tag="PKV56_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX67133.1"
                     /translation="MSIAALFDTPQALPTIPKVAQELIQSFSREDVAIDEIARHLSAD
                     PVLSAKTLRLANSAYFHVSRQVASVDDAIRMLGFVMIRNLVIGCGVTGAFKSVPGLDL
                     PQFWRHSLHTAGASRWLAQTLDRNSDLAFTVGLVHGVGQLVMHAAAPAAMKPLDAECH
                     PLAFERTALEQARLGYHHGEVGAELAARWKFPAEVSDALRAAADPLRAGASSEIGSLV
                     HIAAWRSRVALFDLDAAQAAAGCPGALGRAIGLPVTWLAAEGTLGFEASSGLPAMPPL
                     AELTSGLEAMLD"
     gene            complement(22835..23824)
                     /gene="dusA"
                     /locus_tag="PKV56_04650"
     CDS             complement(22835..23824)
                     /gene="dusA"
                     /locus_tag="PKV56_04650"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HOX67134.1"
                     /translation="MNQQVSAWRLSVAPMMDWTDRHCRYFHRLLTKRTRLYTEMVTTG
                     ALIHGDVKRHLEFNAEEHPLALQLGGSEPADLAHCARLAEDWGYDEVNLNCGCPSERV
                     QRGAFGACLMAEPQLVADGVKAMRDAVSIPVTVKHRIGIDRGESYDFVRDFVGTLSDA
                     GCEVFIVHARNAWLKGLSPKENREVPPLRYEFVYRLKRDFPSLTIVLNGGVTSDEQIA
                     EHLHHVDGVMLGREAYHRPWWLAGWDERHFGDAPSGLDLDAAEAAMVDYMQRLVAQGE
                     PWTRASRHMLGLWNGIPGARHRRQVWSDHRRKDDPPETVSRAARSPLGIAEKV"
     gene            complement(23968..24675)
                     /locus_tag="PKV56_04655"
     CDS             complement(23968..24675)
                     /locus_tag="PKV56_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX67135.1"
                     /translation="MENIPLYSGKNMNLNRFAVLSSVLAGLGFGVMGSAQAGIVWFTD
                     RSAWSAAAGAASFTEDFSSFAADQSFRSSAVALNGMTIQQEGSGDFRNLVETPPTQFD
                     DNNGTSHASMFTDFGATTVRISFSELNGAFGGESWIGTPAEGAQLAVFDGAIELGAMN
                     LTDGAGDFLGYVLTGGSTASSLLFRSVSEISGGTGEGFGFDNLAGVNATAVPEPGTLA
                     LAGLALAGIAASRRRKQ"
     gene            25045..26775
                     /locus_tag="PKV56_04660"
     CDS             25045..26775
                     /locus_tag="PKV56_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67136.1"
                     /translation="MAEAPSNEEEHRAAELRRRAETLLLQAANCPPQSADEIQRLLHE
                     LRVHQIELEMQNDELRRAQNELHASRSRWMELYESAPVGYLTVGPAGEIRQLNRCAAN
                     MLGRARGELLKQPFTKFIFSEDQDTFYLLSQKLLLADDQPSCELRLAAADGKPVWTQL
                     ATTAAHDPDGSRELRIALIDITTRKLFEASVQSQKVAEAASRAKSLFLANMSHELRTP
                     LNAIIGFADLLKRSELTPKQVDRLDKIISAGKHLVEVIDSVLSLSRIDAGKLEPVDAA
                     VDLPRIIAAVVTMLSEQAATKGLHLAAEPVPATAQLRGDPTLLQQTLLNLVMNAVKFT
                     PAGKILVRACIESETADHALVRFEVEDTGIGIDPEVLPRLFSDFEQADNSSIRTYGGI
                     GLGLAIARRIARLMGGDAGVTSELGKGSAFWFTACLRKSASRPDQPLAGPSDAAEAGL
                     LARFAGTSVLLAEDEPLNREVIVSLLADLEVEVAVNGVEAVRLAAAHRYDLVILGVQL
                     PRMDGLEAARRIREIAGYASVPMIALTASGCDDDRSRSMQAGMDAFLTKPVDSRTLFK
                     TVFNSLSRQR"
     gene            26808..27341
                     /locus_tag="PKV56_04665"
     CDS             26808..27341
                     /locus_tag="PKV56_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX67137.1"
                     /translation="MPQDSSETPMPQTQRAAAVVRHLLYAGAIASTLWLGGCAVEVQN
                     RQAAQEIAEMSKPPGSVYLGWRVFRDKCSACHGPDATGPSAGPDLLPRVAELGPRRFV
                     SLVLNRYDWNFPTAKLRGETAERETFVDEIVQRKEGALTMPAWDGEPRVAAHVADIYA
                     YLSARAQGTQGVGRPAQ"
     gene            complement(27449..30436)
                     /locus_tag="PKV56_04670"
     CDS             complement(27449..30436)
                     /locus_tag="PKV56_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOX67138.1"
                     /translation="MTTEDPSAAAAGGPEAAPATPIPLQPTAADRPPLIVGLGASAGG
                     LEAFEQFFRTMTPDSGMGFVLVQHLDPSHASMLTEILQRITVIPVVEAQDGMVVEANR
                     VHVIPPNRDMVIAQGRLLLNVPAEPRGQRMPIDAFLRSLAEDQGDNAIAIILSGTGTD
                     GTLGMRAVFGAGGLCLVQDPATAKFGGMPASAVHAGYVNQVLPVAEMPQALLAIARHL
                     PVARVAPAAPAAGGESGAGARGFGQILALLRAGTGHDFAQYKQSTIGRRIERRMAQHL
                     IEDPEIYARYLKEHPSELKVLFRELLINVTSFFRDAEAFDLMKSEILPALLVGKPAGY
                     TMRVWVAGCATGEEAYSIAMILRELIDTTHPELKLQLYSTDLDDDAIATARAGLYPLN
                     IAQDVSAERLRRFFNQEDAGYRVKKEIREMVVFAVQSVIKDPPFTRLDLLSCRNLMIY
                     LEPDLQNRLIPAFHYALKPGGILFLSPSESVGNHTELFDPLHRKWKFYRAKPTIASTR
                     SVLTSGLSWAVAKSAVESQEQGLRVKEFDVGELARRALLQSFTPAAVVTDLQGNILYV
                     HGETGRFLRPAPGQPTHNVVEMAREGLQIELREALNRATLHSLPTINEAVTIRTDGET
                     LPVDLSVRLLPDIGAGTNVLLVSFQERTQPVPGAPVVHKRKGRASGSAIRAAELEREL
                     DYAKQSMKALLEEQQASNEELKSINEELQSTNEELQSTNEELETSKEELQSVNEELVT
                     VNSELQTKIEQMAGMQDDMKNLLDNIRVGTIFLDRNLLIRRFTREATKVYRLLASDLG
                     RPLADIRSDLSGGDPLADAQAVLDSLVPIERELSTPAGAWYLARIQPYRTVDNVIDGV
                     VLTFTDVTERVHAIATRQARDLAEAVVDAVPEPLVVLDGQLQVMSCNRAFYREFGGSV
                     DDTVGQSVFEVGQRRWDFTEMHDLLESVLPRERSFEGRVIEHDFQRLGVRTLRLSARR
                     VLMPAGSGELVLLVIEPIARAAG"
     gene            complement(30701..31000)
                     /locus_tag="PKV56_04675"
     CDS             complement(30701..31000)
                     /locus_tag="PKV56_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67139.1"
                     /translation="MGQAKIRGSRDARVAQAIVEAGKSKPPVIACNTCGTELTDIAPL
                     DASRLRGIEAAYGAHCQACDQDTWAVRGEVAAVRAFYAALEKAAGAKVQLGIAKP"
     gene            complement(31059..31244)
                     /locus_tag="PKV56_04680"
     CDS             complement(31059..31244)
                     /locus_tag="PKV56_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67140.1"
                     /translation="MTSRHHQRGFAGLLLAVLFLMLLGLPLLYMQSERIEQQRARDKA
                     IKKMLTQPVPPQASAPL"
     gene            31409..31984
                     /locus_tag="PKV56_04685"
     CDS             31409..31984
                     /locus_tag="PKV56_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67141.1"
                     /translation="MKTFLCIGLMALSMTGCGELSYKRGASAQDLEATRKSCQSAGTE
                     KAVEKCLEDNGWVVKKLDDIELFATASVTDDNRNLGTSEPAKGADKPAPGTVARSPAM
                     PGTTAAAGSGGAPVASPAPPPAPPASPLDVYKISSWWKLGAGREAMEADTADCVAQLG
                     SAHKPDNATQQVTRGFVVCMHGKGWKALREK"
     gene            complement(32013..32486)
                     /locus_tag="PKV56_04690"
     CDS             complement(32013..32486)
                     /locus_tag="PKV56_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOX67142.1"
                     /translation="MRVLVAFFANTGNTRFVASEIARRSGAELEEIRDARPHAGACEG
                     LRYIRDSLIGAKPPIEAARRDPSGYDLVILGTPVWAWRVASPMRSYVLQNASRFKQVA
                     FFCTEGGLGDRLVFSELSRLCGQTPVATYQVTEPYLAEPTQQEPLSRFMERLEAT"
     gene            32697..>33195
                     /locus_tag="PKV56_04695"
     CDS             32697..>33195
                     /locus_tag="PKV56_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67143.1"
                     /translation="MFHARGLSKVYRSGEVEVHALRDVDLDIGRGEFVVLLGASGSGK
                     STLLNILGGLDVPTSGEIRFGDQRLDGASEAELTAYRREHVGFVFQFYNLIPSLTVRE
                     NVALVTDIALDPMPVDEAIDLVGLTPRRDHFPSQLSGGEQQRVAIARAIVKRPEVLLC
                     DEPTGA"
BASE COUNT         5724 a        11145 c        10681 g         5645 t
ORIGIN      
        1 aatcgggatc cggtccgccg gcgaacgcgc agcacgcgat tacgtgcgcg accagggcgg
       61 cctgatccac acggcgcgcg atctgcgggg cctggagagc ccgacgcaac tcgctggcgt
      121 gtttggtggc atccgttcaa ggctcgcgca gcacggtcat ccccctgtct acctgagtct
      181 ggacatcgac tgcctggacc cggcattcgc gccgggtacc ggcacacccg aaccgggtgg
      241 cctgacgacg aaccaggtcc tcagcgtgct ggaggaactg gcggacctgc cctttgtcgg
      301 catggattgc gtcgaggttg cacctgccta cgaccatgcc gaactgacca gcaacgctgc
      361 agcgaacttc gtctggactt acctgtgcgg ccgcatcgcc agcgaccccg aagcctgaat
      421 ccgaacggca cccggccacg acccgccctc ctccgtgagc acttcgaaga ccgattcagt
      481 cgatcggtag cgccgtcgtc ttcttcaccg ttcgcagcac gagcatcgag ttcgaacgcg
      541 tcacgcccgg caggctggac agcacgtggc tgtggaagtg ctcgaggtct tcggcgtcgc
      601 ggtaggtgaa gcgcagcagg tagtcgttgg tgccggcgac gtagaagcag tcatggatct
      661 cgggcacggc gcggatcgcc tgctcgaagg tgtccatcgt cgagcgggtc agcttctcca
      721 gattgacgat ggtgtagctg gtgccgtggc gaccaaccgt cttcggatcg atcagtgcca
      781 cgtactgggc tatcacccca ctttcctcga gggccttgac tcgccgcagg cacgccgacg
      841 gcgagaggtg cacccgctcg gcgagttcga ggttggtcag cttcgcgtcg acctgcagca
      901 cctccagcaa ggcgcggtcg atggcatcca gtttcgcgcc aggatcctgt tttcgcatga
      961 ttcttcgaat tttgttgatt caacgaatta ttagtgcgca gactcgggta atcagcggct
     1021 caacgcgcaa gctaattgcg tgaatcagac actaaactgc tgtgcgatcg cctgtcctct
     1081 ggcgcagtgc aacaaggaag tttcgatgca gcagaacctc gatgcctact ggatgccctt
     1141 caccgcgaac cggcagttca aggccgcgcc acggatgatc tccggggcct ccggcatgca
     1201 cttcagaaca ccggaaggac gagaagtcct cgatggcatt gccgggctgt ggtgcgtcaa
     1261 tgccggccac ggacgccctc gcatcaccga agccatccag aagcaggctg ccgagcttga
     1321 cttcgctccg cccttccaga tgggccatcc gaaggtcttc gagctcgcgg aacggctgat
     1381 cgaactcacc ccacccggac tcgacaaggt cttcttcacc aactcgggct ccgaatcggt
     1441 cgagaccgca ttgaagatgg ccatcggcta ccaccgcgtc cggggtgagg gacagcgcac
     1501 gcggctgatc ggtcgcgagc ggggatacca cggcgtcaat ttcggtggca tctccgtcgg
     1561 tggcatggtg gccaaccgga agatgttcgg cccgatgctc ggcggggtcg atcacatcag
     1621 gcacacccac gacccggtgc gcaacgcgtt ctcgaaaggc cagcctgagc acggcgccga
     1681 actcgccgac gatctcgagc gcctgatcgc gctgcacgac gcctcgacca tcgcggcggt
     1741 gatcgtcgag cccgtcgcgg gatccaccgg cgtgctgctg ccgccgaagg gctacctcga
     1801 acggctgcgc gccatctgcg accgtcacgg catcctgctg atcttcgacg aggtcatcac
     1861 cggattcggg cgcctcggct cgccattcgc gggcaatcat ttcggtgtga cgcccgacct
     1921 gatgaccgtg gccaagggat tgaccaacgg ctgcgtgccg atgggggccg ttttcgcgaa
     1981 gcgcttcata cacgatgcct tcatgaacgg ccccgaacat ctgatcgagt tcttccacgg
     2041 ctacacctac tccggtcacc cgctggcgtg tgccgcgtcg ctcgggacgc tcgacaccta
     2101 cgccgaggaa ggcctgctga cgcgcgccag ccgcatggcg gcgaccttcg aggctgcggt
     2161 gcattccctc gcggggctgc cccatgtcat cgacatccgt aacatcgggc tcgtcggcgg
     2221 catcgaactc gccccgatag ccggcgatcc cgggaagcgc gcattccagg tgtttctcga
     2281 ctgctgggag cagggagttc tggtgcgcac gaccggagac accgtcgcgc tgtctccgcc
     2341 gctgatcatc gagaccggac acatcgagcg gatcatcgat acgctgaccg gggcgatccg
     2401 gcgggccgcc tgagatcggg caaccggaca agaccccagc cgctaccgca ctgccctgcg
     2461 tcgactcatg agcttcctca gcaaggacct cgacctcgcg agcaactcct attacgacgc
     2521 cacggtcgag cgcactgccg cccgatcggc gctggccggt gacatcgagt gcgatgtcgc
     2581 tgtcgtcggc ggcgggctgg ccggactgtc ggccgccatc gaactcgccg atcgcggcca
     2641 cgacgtggtg ctgctcgagg ccaggcgggt cggctggggc gcctcggggc gcaacggcgg
     2701 ccaggcactg gcaggtctgg cctgcgacca ggacaccatc gaggcgcagc tcggtcttga
     2761 cgaggcccgc cgggtctggg cgatgacgct ggaggcgctg gccctgatac gccagcggtg
     2821 cgagcgcttt cacatcgatt gcgactggcg cagcggttac ctgagcctgg cggtgaacgc
     2881 ccgcaaggca cgcgatctgc gggcgtggca cgcgcgcatg cagtctctgt acgggttcga
     2941 gacgcggtgg atcgagcccg ctcagctgtc atcgtggatc gacagccccc gtttccacag
     3001 cggcctgcac gatcccgcgt cgggtcacct gcacccgttg aagtacagcc tgggcctggc
     3061 gcgcggcgcg gccgaactcg gcgtgcgcct gttcgagaac tcgccggtca cgtcggtcga
     3121 accggcagcc actgccggca gcacggtgcg ggtgcgcacg gctgcaggca gcgtgcgcgc
     3181 ttcgcacgtc ctgctggccg gaaacgtcta cctgcagggc gtcgcgccgg tgctggagtc
     3241 gcggatcatg ccggtcggca cctacatcgt cgcatcgcag cgtctggacc ctgcgctcgc
     3301 gcagtcgctg attccttcgg gctcggccgt ctgcgacacg aacttcgtgc tcgactactt
     3361 ccgtgtggca tcgaaccccg cgtcaacttc gccggacacc gagcccagag agcatcgcat
     3421 gctgtatggc ggccgcgtca gctacagcac ggcgacgcca cgcgacctca agtccagcat
     3481 gcagcgccgg atggcatcga cgttcccgca actggcgacc accgatgtcg agttcgcctg
     3541 gggtggtttc gtcgacatct cgatgaatcg cgcgcccgac ttcggccggc tgtccgaccg
     3601 cgatccccgg tcgggcgcgg gatacgcgaa cgtctattac gtccaggggt tctccggcca
     3661 tggcctggcg ctgaccggca tcgcgggccg catcgtggcg gaggcgatcg acggcgacac
     3721 acgtcgcttc gacaccttca gccgcctccg tcatcatgca tttcccggcg gtaagctcct
     3781 ccgaacaccg gcgctcgtcg caggcatggc ctaccaccgg ctgaaggatc tgctttgaac
     3841 caccctccca ggcacattca ttgcactgcc tgcaggacag gctctcgatg ccgcacgctc
     3901 cccttgcatt cgccatgacg cgcaaaccct tcgtcctcgt gccggcatgc catcgcatgc
     3961 tgggtgacca cctcaatcac accgtcggca agaagtacac cgacgcggtg cgactcgccg
     4021 gctgcgtgcc gctgctgttc acgtcgatcc agggccccga tgaactcgat gacctgctcg
     4081 atgctgcgga cggtgtcctg ctgaccggtt cgccatcgaa cgtccatccc agccatttcg
     4141 gcgagatcgt ctatgacccg agcctcccgc tggaccctgc ccgcgatgcc gcgaccctgg
     4201 cgatgattcc ggcggcgatc cagcgcggga tgccgctgat ggcgatctgc cgcggattcc
     4261 aggaagtcaa tgtcgctctc ggaggcagcc tgtaccaggc cgtgcatgaa gttccgggtc
     4321 acctcgatca ccggggcgtt gccgaaggtc cgccttccga ggtctacggg ctggcgcatc
     4381 aggtcgcgct gcaacccggc ggcctgctgg aggagttgct tcagtgcgcc gcgatcgagg
     4441 tcaattcggt gcatggccag ggcatcaatc gcctggcgca ggcgctgcgt gtcgaggccc
     4501 gcgcgcccga tgacctgatc gaggcattca ccctggcgaa tgcatccagc ttcctgctcg
     4561 gcgtgcaatg gcatcccgaa tgggaagcgc acctgaatcc ggtatcgacc cgcctgttcg
     4621 aggcgttcgg gcgggcctgc tgcaactttc gtgatcggca caggggtccc gacgggatca
     4681 ggtgatgcac actgatcctc aagcacaaga cgacagcaac tgactcaagg tgactcccca
     4741 tgccggcccg agaaaatttc acattcagcg atctggaaaa ctggctcaac gagcggcgtg
     4801 tgaccgaaat cgaatgcctg gtacctgacc tcaccggcgt ggcacgcggc aagatcctgc
     4861 cccgcgagaa gttcaccgag gatcgcggca tgcggctccc cgaggcgatc gtcgcgatcg
     4921 gagtgaccgg cgaattcccc gaagaaggcc cgtactacga cgtgatccac gcgaccgacc
     4981 gcgacatgca ccttcggccc gacgcgtcga cggtgcgcat cgtgccgtgg gctgccgatc
     5041 cgacagcgca ggtcatccac gactgtttcg accgcgacgg caagatgatc ccgttcgctc
     5101 ctcgatcggt cctgcgccac atctgcgacc tctacgaagc cgaggggtgg gccccggtgg
     5161 tggcgcccga gctggagttc tacctgatcg agcgcaacgc cgaccccaac acgccgctgc
     5221 gcccaccgaa gggccgcagc ggccgagccg aaacctcgcg tcaggcgtac tcgatcgatg
     5281 ccgtcaacga attcgacccg ctgttcgagg acatctacgc ctactgcgag aagatggagc
     5341 tgaatgtcga cacgctgatc cacgaggtcg gcgccggcca gatggagatc aatttcttcc
     5401 acgaccaccc gctcgggctg gccgacgagg tgttcttctt caagcgcacg atccgcgaag
     5461 ccgcgatgcg gcacgagatg tacgccacct tcatggccaa gccgatgtcc aacgagcccg
     5521 gcagcgcgat gcatgtgcac cagagcgtgc tcaacaaggt gacgggcaag aacatcttca
     5581 gcaaccccga cggatcgccg tccaaggagt tctactggta catcggcggc ctgcagaaat
     5641 acacgccggc cgctatggcg ctgttcgctc cgtacgtcaa ttcgtaccgc cgtctcgcac
     5701 gctcgaccgc ggcgccgatc aacatccagt ggggaaccga caaccgcacc gtcggcatcc
     5761 gatccccggt ggcgacggca gcagcgcggc gcatcgagaa ccgcgtgatc ggtgccgatg
     5821 ccaacccgta cgtcgcgttt gccgccacgc tggcctgcgg ttacctgggc atcaagaaca
     5881 agatcgagcc ggccagcgag tgcaagggcg acgcctacct ctccgaatac cagctgccgc
     5941 gttcgttgag cgaggcgctg ggctggctgg ccgacgaaaa ggacctgcac gacgtgctcg
     6001 gcaagtcctt catcacggtc tactcggaga tcaaggagat cgagcacgac gagttcatga
     6061 aggtgatctc gccctgggag cgtgaacacc tgctgctcca cgtttgacgc gctcctttgc
     6121 tgcgccaact gaaacccgaa accggactgg actctcgatg aacgctgcac ccgacacgac
     6181 cgcaacgcgc accacggccg agtggcaggc tgcggattcg gcgcactacc tgcacccgtt
     6241 caccgacttc aaggcgctgg cgagcaaggg ctcgcgcgtc atcacgcgtg cggacaacat
     6301 ctatctgtgg gacagcgacg gggcgaagat cctcgatgcg atgagcggac tgtggtgcgt
     6361 caatgtcggc tacggacgcg atgagctgat cgacgcagcg accgcgcagc tgaagcagct
     6421 tccgttctac aactcgttct tccagacggc cacgccaccg gcgatcgaac tggcagaact
     6481 gctcgccgaa gtgacgccgc cgcagttcaa ccatgtgttc ttctcgggtt cgggctcgga
     6541 aggcaacgac acggtggtgc gcatggtgcg gcgctactgg gacgtgctgg ggcagcccga
     6601 acgcagcgtg atcatcagcc gtcgcaacgc gtatcacgga tccaccgtgg cgggcgcgtc
     6661 gctcggggga atgagctaca tgcacgcgca aggcggactg cccattcccg gcatcgtcca
     6721 catggagcag ccgttctggt tcctgcacgg ccatggcaag acccgcgacg cattcggact
     6781 cgaagccgca agctggctcg aagcgaagat cctcgaagtc gggcccgaca aggtggcggc
     6841 cttcatcggc gagcctgtgc agggggccgg tggtgtcatc gttccgcccg ccacctactg
     6901 gcccgagatc cagcgcatct gcgacaagta cggaatcctg ctcgtgtccg acgaggtcat
     6961 ctgcggtttc ggccgcaccg gcaactggtg gggctgcgag acgatggggt tccgacccga
     7021 cctgatgacc ttcgccaagg gcgtgacctc gggctacata ccgctgggcg gcgtgatggt
     7081 cggagatcgc atcgcgaagg tcatgatcga gcagggcggc gagttcgagc acggctacac
     7141 gtacagcggc cacccggtgg cctgcgctgt gggactggcg aacatcaggc tgatccagcg
     7201 cgacgggctg gtcgagaagg tccgcaacga caccgggccc tacctggccg agcatttccg
     7261 ctcgctcgcc gagcaccccc tgatcggaga ggcgcagtcg atcggattga tgggcgcgct
     7321 gcagatcgtc agggacaagg ccagcggtgc gtgcttcgac ccggcactcg aggtcggcat
     7381 gatctgccgc ggccattgct ttgccaacgg cctgatcatg cgcgccgtcg gcgaccgcat
     7441 gatcatcgcg ccgccgctca tcatcacgcg cgcccagatc gacgagatgg cccgcttgat
     7501 acgcttgtgc ctcgatgcca cgctgcgcga cctccagcag cgcgacctga tctgacctgg
     7561 agcccgccga ccgtggcaca gcccttgcct gtcgtgtaca cgccggcgcg ctgccgtgag
     7621 attgcccttc cctgttgatg ccatgccgtt tggagattcc gccatgaccc gaccgacccg
     7681 ttcctccttg ccgcctgttc gcaatgcgct gatggcgctt gccgccggtg cagccgctgc
     7741 taccgcgatg tgctgggccg ggcccgcggt cgcccaggaa gaggagaagg tcctcaacat
     7801 ctacaactgg tccgactaca tcgcgcccga caccatcgcg aacttcgaga aggaaaccgg
     7861 catcaaggtc cgttacgaca atttcgacaa caacgaaatc gtccacgcca agctggtggc
     7921 gggcaagacg ggctacgaca tcgtcgtgcc ctcttccaac tgggccaagc tccaggccga
     7981 cggtggcctg ctgcgcaagc tcgacaagtc gctattgccg aactacaaga atctcgaccc
     8041 ggtggtgcag gcacagattg ccaagctcga tcccggcaac gaatacatgg tggactggct
     8101 gtggggctac accacggtgg gcatcaatgt ggacaaggtc aaggccgcac tcggcggcct
     8161 gccgatgccc gagaacgcct gggacctggt cttcaagccg gaatacgtgt cgaagctcaa
     8221 gtcgtgcggc gtgtcctacc tcgactcggc aaccgaggtc gttccggctg ccctgcacta
     8281 cctcggcaag ccgtcgttca gcaaggtggc aggcgactac gcgcaggcag cggccgtgct
     8341 gaaggcggct cgtccgagcg tcacgctgtt cagttcctcc ggctacatca acgacatggc
     8401 caatggcagc atctgcgtcg ccctgggttg gtcgggtgac atcaacatcg cgcggcaacg
     8461 cgccatcgac ggcaagaccg gtcagaacat ccaggcgctg atcccgaaga gcggcggcat
     8521 cctgttcttc gacgtgatgg tgatccctgc cgacgcaccc catcccggca atgcacacaa
     8581 gtggatcaac ttcatcctga agcccgaggt gcacgccagc atcaccaaca aggtgttcta
     8641 cgccaacccg aacgcggcct cgcgtccgct ggtcaagccc gaggtcgcca acaacccgac
     8701 ggtgttcctc tccgaggccg acatgaagcc gatggccgcc cccgactcgc tcggcaacga
     8761 cctgcggcgc ctgatgacgc gcaccttcac cgccttcaag accgggctgt gagcctgtcg
     8821 aactgagcga gactgtccat gaacatcgga agcaccccga cgagcgacag ctcgaaattc
     8881 ctgcagatcc gcaacgtcac gaaggacttc agcggctaca aggctgtcaa caacgtcagc
     8941 ctggacatca gcaagggcga gatcttcgcg ctgctgggct cgtcgggctg cggcaagtcg
     9001 acgctgctgc gcatgctggc tcgtttcgag acgccgactt cgggccagat cctgctcaac
     9061 gggcaggatc tggcgggact gcctccgtat cagtgcccga tgaacatgat gttccagagc
     9121 tacgcgctgt tccctcacat gacggtgtgg gacaacgtgg ccttcggccc gcggcgctcc
     9181 ggcatgagca aggaagaggt ggctgcgcgc gtcgacgaga tgctccacct ggtgcaactg
     9241 ggcaagttcg cccagcgcaa gccgcaccag ttgtcgggcg gccagcagca gcgtgtggcg
     9301 ctggcacgca gcctggcgaa gaagccgcag ctgctgctgc tcgacgagcc gctgggcgca
     9361 ctcgacaaga agctgcgcga ggaaacgcag atcgagctgg tcaacatcat cgagaacgtc
     9421 ggcgtgacct gcgtgatggt cacccacgac caggaagaag cgatgacgat ggcttcgcgg
     9481 attgccgtga tgagcgaagg ccgattcctg caggtcggcg cgccgagcga catctacgaa
     9541 accccggcca cgcggttcgt cgccgacttc atcggcaacg tcaacctgat ggaaggcacg
     9601 ctgaccgcgg accaccccga ccacgtggtc atcgagtgtg ccgactgcaa gcactatgtc
     9661 gggcacggca tcacgggctc cgaaggcatg gcggtgacgg tggcgctgcg tccggagaag
     9721 atccgcatcg gccgccagcc gctgccgccc agcgacggcg aaccggcggg cggcctgaac
     9781 cgcgtgcggg gcacggtcaa ggacatgggc tatttcggca gcttcacggt ctaccacctc
     9841 cagctcgcga gcggcctggt gatgaagatc agcgaaagca acaccgagag gcaccgcgaa
     9901 gacgaactga cctggggaga caccgcatgg gccagctggt cgccgctggc gcatgtggtg
     9961 ctgacagcat gacaccgtcc agtttcccca aggtgagcga ccgccgaggg gcgggagcat
    10021 ccgaagtgcg cgggagatgc caatgaaagc gatgctgtcc cggctgctca gtggccgctc
    10081 cgccgtcatc ggcatcccgt atctctggct gctgatcttc ttcctgctcc cgttcctgat
    10141 cgtcatgaag atcagcgtgt cggagatgga gacggtcaac ttcaaggaca tcatcaccta
    10201 caccgatgac gctctcgccc tgacgctgcg cttcaccacc tacctgttca tcacgcagga
    10261 cgacctctac tacaagacct acctgtcgag cctgaagtac gccggcgtca ccacgttctt
    10321 ctgcctgctg atcggctatc ccttcgcgta tttcatggcg cgcgccaagg cgacggtcca
    10381 gcccgccttg ctgatgctgg tgatgctgcc tttctggaca tcgttcctgc tgcgcgtcta
    10441 cgcatggaag ggactgctca gcgagaacgg ctgggcttac gacgtgctcg tcggcagcgg
    10501 gctcgaccag gtgcttgcgg cgctcgggct cataccggcc cccgcgcagc tgatgaactc
    10561 tccgttctcg ctggtgctcg gcatgaccta cacctacctg ccgttcatga tcctgccgct
    10621 gtactcgaac ctgtcgaaga tggacatgcg cctgctcgag gctgccgacg atctcggtgc
    10681 gacgccgttc gtcgcgttct ggaagatcac ggtgcccttg tcgaaggccg gcatcatcgc
    10741 cggctcgatg ctggtcttca ttccgtgcgt cggcgagttc gtcattcccg aactgctggg
    10801 cggtccgcag acgctgatga tcggccgcgt gctctgggac gagttcttca gcaacaacga
    10861 ctggccgatg gcctcgtcgg tggcggtggt gatggtgctg ctgatcatcg tgccgctcgc
    10921 gatcttcaac aagtaccagg ccgaagcgac cgaggtgcgc aaatgagcgg gtccctcttg
    10981 aaccggtggt tcggcaaggg ctggctggca ttcggcttcg ccttccttta cctgccgatc
    11041 gtcgcgctgg tcttgttctc gttcaacgat tcgccggtgc ccaacgcctg gcgtggcttc
    11101 acgctcaagt ggtacgaggc gctggcggcc gaccacgaga tgatcgccgc gttctggctg
    11161 tcgctgaaga tcgccttctt caccgcctgt gcgtcggtcg tgctgggcac cctggcggcg
    11221 ttcgctctgg tcaagtaccg gcgattcacc ggccgcacgc ttttctcggg aatggtcagt
    11281 gcgccactgg tgatgcccga ggtcatcgtc ggtctgtcgc tgctgctgat gctggtttcg
    11341 atgcagcgcg cgctgggctt tccggaacgt ggggccctga ccatctggat gggtcacctg
    11401 ctgctcggca tggcctacgc cacggtggtc atacaggccc ggctgcaaga cctgaacccg
    11461 cagctcgagg aagccgccat ggacctcggc gcccggccct gggaagtgtt cacgctggtc
    11521 acgctgccga tgatcacgca gtccctgatg tcggcctggc tgctgacctt cacgctgtcg
    11581 ctcgacgacg tcgtgctgtc ggctttcctt tcgggaccgg gctcgacgac gatgccgctg
    11641 gtgatcttct cgcgtgcacg gctgggcctg aatcccagcg tgaacgcggt ggccaccgtg
    11701 atcgtggtca tcgtcgccat cggcgtcgtc gccgccagct atctcatcgc ccgtgccgag
    11761 cgcaatcgct cgctcgacat ggccgcggcc aaccgggcca gctgaccagg ctggtctcgg
    11821 atccctccga agcacatccc ctacaagtaa cagcacgagg agttctcatg aagacctggc
    11881 actgcaccgt actgacactg gccatcgctg cggctttccc gctgcaggcg tccgcccagt
    11941 ccaacgagga gttgctcaag gaactgcggg ccctgcgcga ccgggtcacg caactcgagg
    12001 aaaagctgaa gtccgccgct gtgccccagg cgccggcggc tgcgattccc ccggccgccg
    12061 tggcgccggc aggcatgaca ccggaccagg cccgcgagat ggcacggctg gccctgaagg
    12121 tcgacacgct cgaagaagag cgcgagaact cgaacttcaa ggggctgacg atctccggca
    12181 gcgtcgaccc gaccttcatc tacaaccacg cccagaaccg ggccggattc cagcttctga
    12241 attcggcgac caccggcccc tacgcctacg acaactcgtt cttcggatca gcctcgctcg
    12301 acttccagaa ggaactcgag ggtggcacca agttccgcgt gacgctgcag cccgatcgcg
    12361 gcgtcggagc acacatcaac gccggatcga tcgtccacga ggcgagcgtg tcgattccga
    12421 tcgatggcga cgaaacccgg ttgatcgtcg gccagattcc cgactggtcg gggtacgagc
    12481 tgctggcgtc caacgaaaac aagctggtca cgcacaacct gctgttcgac ctgatcgcgc
    12541 cgctggccta caccggcgtg ggcctcgagg tcacgcgcgg cgactgggtg atcaagggcg
    12601 tcgtcggcaa cttcaactcc accaagcgcg agcccgacga gaagtctccg atgctggcct
    12661 atcgcggcga ctactccatc agcgagtact cgggcatcgg cttctccggc gtgcacggca
    12721 aggctgccaa tttcgtgcag gaaaccggcg acaccacgct gcacctgttc gaagtcgacg
    12781 gctactacat gcgcggcgac tggaccgtcc agggccaggt cagcttcggc acgcagaagg
    12841 acgcggccat tgccctgagc agcagcgatc ggcccaagga cgccgagtgg tggggcctgt
    12901 ccggcctgac ggcctacaag ttcacgccac gcctcgaagg cgtggtgcgc gccgacttca
    12961 tctacaaccg caagaacggc ggtggcctgc tgggcttcgg tgccgactcc ctcaacggta
    13021 tcgggccggg cttcgtcggc tacgacgccg agggtgccgc cgaggctcgc agcagccagc
    13081 acggttccaa ccgctacgcc ttgagcgtcg gcggcaatta ccgcttcaat gaatacacgc
    13141 tgttcaaggc cgagtaccgc ttcgacgggt cgacccgtcg cgtgttcgag tacgtcgacg
    13201 acggcaactt caagaagacc aaccacctct tcggcgcctc ggtcgtcgtg tcgttctgat
    13261 ccatgacgac cgccgcgacc cgtgactggc acgcgctggc cgcagcgctg cgcccggatg
    13321 ggcgagtctt catcgaaggg gtgcggcggg ctgcggcagc cgaggaaacc ttcgcctgca
    13381 tctcgccgat cgacgggcgc acgatcgcgc aggtcgcacg cggccaggcc gccgacatcg
    13441 acgcggcggt gacctcggca cgccgcgcct tcgacgacgg tcgctgggcg caccggccac
    13501 cggcggcacg caagaaggtg ctgctgcgct ttgccgagaa catccttgcc gccaaggagg
    13561 aactcgcgct gctcgagacg ctggacatgg gcaagcccat acagcacagc ctgggtgtcg
    13621 acgtgccttc ctccgcccgc tgcatcgcgt ggtacgcgga ggcagtcgac aagatctacg
    13681 acgagatcgc gcccaccgca cgcaacgcgc tggcgctgat cacgcgcgag ccgatgggcg
    13741 tcgtcggtgc catcgtgccc tggaactacc cgatgatcat gtcggcctgg aagctcgggc
    13801 cggcgctggc ggcgggcaac agcgtggtgc tcaagcccag cgagaagtcg ccgctcacgg
    13861 cgatgcgcct ggccgagctg gcggtcgagg cggggttgcc gcccggggtg ttcaacgtgg
    13921 tcccgggctt cggacacgag gccggcgagg cgctcgcgct gcacatggag gtcgacgcca
    13981 tcggcttcac cggcagcacc cgcgtcggcc gcaagatgct cgagtacgcc ggacgcagca
    14041 acctgaaacg cgtctacaac gagctcggcg gcaagtcggc gttcatcgtg ttcgacgatt
    14101 tcaccgacat ccctcgcgcc gcctgcacgg tggccggcag catgttcttc aaccagggcg
    14161 aaagctgcaa cgcgccctcc cgcgtgctgg tccacgagag cgtggccgac gaattcatcg
    14221 ccatcgtcgc ggccgaggcc ccgacgtacc agcccggcga cccgctggga ggatcggcgc
    14281 ccatgggcgc gctggtcgac gacgggcaac tgcgcaccgt gctcggctac atcgatgccg
    14341 gccacacgga tggcgcaagg ctgatcgcgg gtggccagca ggtccgggcc gagaccggcg
    14401 gctgctacgt cgagccgacg gtgttcgacg gcgtgaccaa ccagatgaag atcgcccgcg
    14461 aggagatctt cggcccggtg atgtcggtga tccgtttcaa gacggaagtc gaggcgattg
    14521 cgatcgccaa cgactccccc tacggtctgc aggccagcgt ctggagccac cagatcgacc
    14581 gcgcgcaccg cgtcgctcgc tcgctgcgtg ccggcacggt gcatgtgaac cagtacgacg
    14641 aggacgacat caccgtgccg ttcggcggat tcaagcagag cggcaacggg cgcgacaaat
    14701 cgctgcacgc gttcgacaag tacaccgaac tgaagaccac ctggctgcgc atcgacccat
    14761 gaacctctcg cccaacgaac agctgatggc ccgcaaggcc gccgccacgc cgcgcggggt
    14821 cggcgtgatg ggcaacttct tcgccgacca cgccgagaac gccgagctgt gggacatcga
    14881 gggtcggcgc tacatcgact tcgccggcgg catcgcggtg atgaacaccg gacaccgcca
    14941 cccgcgcatc gtcgcggcca tcgaggcgca gctcaagcgc ttcacccaca cctgctacca
    15001 ggtcgttccg tacgagggct acatcgccct cgcggagaag ctcaacgcgc tgacaccggg
    15061 caagcatgcg aagaagaccg cgctgttcag caccggcgcc gaagcggtcg agaacgcgat
    15121 caagatcgcc cgcgcccaca cccggcgtgc cggcgtcatc gccttcagcg gcgccttcca
    15181 cggccgcagc ctgttcgcgg tcgcactcac aggcaaggtg cagccgtaca aggcccagtt
    15241 cgggccgttc cctcccgagc tctaccacgt gcccttcccg tgccactgcg ccagcctcga
    15301 agaggtcaag aaggctgtgc agcagctgtt caaggccgac atcgaaccga gccgcgtggc
    15361 cgcgatcatc ttcgagccgg tgcagggcga aggtggattc aacgtgatcc agcccgccgc
    15421 cgtgcaatgg atacgcgagc tctgcaacga gcacggcatc gtgatgattg ccgatgaagt
    15481 gcagaccggc ttcggccgca ccggcaagct gtttgcgatg cagcacttcg agtcgctggg
    15541 cgtggtgccc gacatcctgg tcagcgccaa gtcgctggcg gccggcctgc cgctgtcggc
    15601 ggtgaccgga cgtgccgaca tcatggacgc gccgaatccc ggcggtctcg gcggcaccta
    15661 cgccggcaac ccgctggcca tcgccgccgc acatgcggtg atcgaggtga tggccgacga
    15721 gggcctgccc gagcgcggca ggcagctcgg tgaccggctg aaggcccagc tggagagcat
    15781 gcgagccgag gtgccgcaga tcgccgacgt gcgcggactg ggtgccatgg tcgcggtcga
    15841 gttcaaccgc gccggcaccg ccgagcccga tgccgacttc acgcggcgcg tgcaggccgc
    15901 cgcgctggcg cgcgggctga tcgtgctgac ctgcggcgtc tacggcaacg tggtgcgctt
    15961 catgttcccg ttgaccattc cgaccgcgca tttcgacgag gcgcttggca tgctggtcgg
    16021 cgccctgcgc gacgccatcc agcccgcgtg agtcgatgaa gacgtctctg ccggacatcc
    16081 agcctgcagc cttccaggcg ctgcgcgaac gccttgctgc cgacggcgtg cacacgctga
    16141 tggcgcagtt caccgacctg cacggcgtgg cacgcggcaa gctggtgccg ctgaaccagc
    16201 tggaactgct gatgaccgaa ggtgtcggct ttgccgggcc gtcgatctgg ggcaccggcc
    16261 tgccgcgcat cggaccgcgt gccgagtact acggccgcgg gctgatcgac tcggttcgcg
    16321 ccctgccctg gatgcccggc atcgcccgcg tcgtctgcga cggcttcgtg gccggcgagc
    16381 ccttcgaggc gtgccctcga caggtcctga agcgccagat ggcgcggctg gcggaacgcg
    16441 gttggacgct gcgcaccggc atcgagcccg agttcttcct gctgcgccgc agcgcagacg
    16501 gcttcgagcc tgccgacccg cacgaccgcc tcgacaagcc ctcctacgac ctgaagagcc
    16561 tgccccgcca gcgcgagttc ctgcggcagc tcgccgacgg cctgacgcgt tgcgggctcg
    16621 atgtcttcca gatcgaccac gaagacgctc acggccagta cgagctgaat ttccaccacg
    16681 ccgatgcgct cgccagcgcc gaccacctga tgctgttcaa gctggccgcg catgcgctgg
    16741 ccgagcagca cggcatggtc ttctcgatga tgccgaagcc tttcgccaat cagcccggca
    16801 gcggcatgca cttccatgtg tcgctgtgga atgcgtcagg cgccggtcga acagggctcg
    16861 acgcacgctg cgtgttcgac gaccccgcgc acgacctgtc ggtcaccggg cgccagttca
    16921 ttgccggggt gcttcaccat gccgacgcgt tgtgtgccat cgccgcgccc accgtcaaca
    16981 gctacaagcg actgaccgtc ggagagtcga tcaccggcac cacctgggca ccggcctatg
    17041 tggcccacgg cccgaacaac cgcaccgccc tggtccgcac gctcaagggc cgcttcgaat
    17101 ggcgcgtgcc cgatgcgtcg gccaacccct acctggccac cgccgcgctg atcgctgccg
    17161 ggctcgacgg gatcgaccgg aagctcgatc ccggcctgcc ctgcaccgac gacctgttcg
    17221 agctgtcgct gtcgcagatc cacgaccggg gcatcgcggt gttgccgcag agcctgtcga
    17281 gcgccgtgga tgcactcgaa cgcgacgaag tgattcgctc ggcgctgggc gccaccctgt
    17341 cgagcgagtt catccgcctc aaacgcgccg aatggaccga gtacgcacgc cacgtcagcc
    17401 catgggagat ggcgcgctac gcagcggcgt tctgatcgcg caggcggtcg ctgccttggt
    17461 gggctcactg gtgtttaacg aacccattgc attcttgagg cgtttccgta gttccccgtg
    17521 gtacttgaac cgggatgact gcatctgcca gcgccaggat cagcaaccgg ccaagagccg
    17581 cagctcgcgg ccgacagctt cgcggcgaac tcaagggcat cgcggcggtt cgggatcagc
    17641 ccgtcatcga gaagatcctc acgcacctgg gtctgttggc tcgggctcac ccgcgcgctg
    17701 cgagcggcct gaccctcaac gctgtggtca cgacgcttcg tccaaggagc ggtgcgcgtg
    17761 catcgccact acttgaagtc tgcactcagg tcgtaggcct gcacgcggtt ttccagcatg
    17821 tgcggcaaga tcaacgtgcg tgactcgggc aggtacgcca agtcggcagt accctgtttg
    17881 aattgccgca gcagccgcgt gtcgccgttg gcagcaacgt gaagaatctt gcccgcgacc
    17941 cagtcgctga cgacgtagcc gccttggcca tcggactcca acccgtcgag gttgccggtg
    18001 ggcttggacg tgatgagggt cttggcaccg gtggtcagat tcaggcggta aaggtggccg
    18061 ggaaccttgg tagagaagtt ggcctcgggc ttgccccagg ccgccacaac caaactgtcg
    18121 ccttcgacga gcaggccgtt ggggtactcg agttgctctc cctcggcaaa ggtggtgggt
    18181 atgccatctt tgatgcggtg gatcttgctg gtgagcatgt ccgagacata caccgcgccg
    18241 tcggggccgg tggccacgtc gttgagaaac ttcgcgccgt cgaccttgat gcgtgacttg
    18301 atggtgccgc tggagatgtc gatcgagacc atttcatcga catcggccac ccacaacgtg
    18361 ccgccgtggc tgcgcaggcc cttgggcgag ttcagtcccg tgacccagtt gagcgccagc
    18421 accttgccgt cgcgcgtgag tttggagatg acgccgttgc catccttgtc catcggcgtg
    18481 ccgcccgggc gcagaccgac ctgcgaaacg aacagcaggc cgctttcgcg atcgaggtat
    18541 gcgctttcgg gcgcgttaag cccctcgctc accgaccacg gctcgatgcg cggcgttgcg
    18601 caggcggcca acagcgcgag cagagtagcg acggcgccga aacggcgggc aaaggtgaga
    18661 caggtaagca tcgccattct tatttccttg tcgccaattg tgtgacgggc cgcgagtctg
    18721 gcagcttcca cgccggttat caacggcgcg atctcgccgg gccttttcag gggattttca
    18781 aaggcccttt tacttgctat tggcaccccg aacacggtac ctgacgctct tgaatcggtc
    18841 cttgaatttc gctgcgatct gttgagcgtt gattgcaccg ctatcgccaa gctgcacgcg
    18901 cgggtggtgc tgcatgggcc ggatgggccc gccaacacag acacaacccg ccgagtgcga
    18961 ggcaaactgc ggatatcacc gctcggtgcg gccaagttgc gcctgactgc tcaagcgcgt
    19021 gttcgcgctg cacctcgagc gctgcccgac ctgcggcggt tagctgaaga tcatcgcagc
    19081 gatcctggag caaccggtga tcgagaagat cctcacgcac gcgcacctgc aggcccatgc
    19141 gagtgactga ttcgaggcga tgaactcagg gtagtgaatg tcgctgatgg gtcgtgaccg
    19201 gccgatcggc acaacctgac cgccgctacc cagttctcag agtcgccgcc tccgggcaac
    19261 gcattgcggc tcgaacggcg cagctgaaac gcagccatca gaggctcttc acccccccgt
    19321 ttgctctctc acgatctcgc ttctcacagg cgtgtgccct gtggtcaggg tcaaatcgag
    19381 agcgaaagtg ggttgcgcag ccgctgcgcg accctggtca ggttcgcagc ccccggatcg
    19441 gggcagccgc acgcacgcgc ggcttgccca ccagagcggc gggcacacgg ggtcggcgct
    19501 cgcgcttgac cttggccacc tcgggcgctg gttgggccag ctggcccagc gggtgcgtga
    19561 gtcggcaggc cagatccatc acgcgctcgg gttcgacacc cagcgtgttg atcagcaact
    19621 cgatgatctc cgagcgcggg ttggcgagcg tgggctcgat catcatcacc gcggcagcca
    19681 ccgccagggc tcgttctcgg gaagactcat ccggcagcat gtcgccgagt gcgttcagtg
    19741 cctcgacagg cgccaccgcc gcgatgcggg cctcctcgcg cagcaggcgc cgccagtcta
    19801 tggacgcgcc gagcccgtct ccctgcgcac cctcgaccac gcgcagatcc gacagcagcc
    19861 gcgccagacg gaaactgcgg cgctcgaacg tgccgatgga caccatcccg gccagcacca
    19921 cgcgacagac cgcctgtgca aagccgccct cgccgatgtg cggcagcagc tcgcgccgca
    19981 gttcgtcgat ctcggcctga gcgcgcgcgg cagccagctg ttccgccggt gcatccggcg
    20041 gcagccatgc gccgatgccc atcgggccgt agaccatgcg cgtccactgc accgtggcct
    20101 gatcacgcag atcgcgatac atgttgaggc tgttctccat cgacttggac aaggcctgct
    20161 cccacacgcg accgggatgg tcgtcgggca gcgcatgccg gctctcgcgc accttgcggg
    20221 ccagttcctt gacaggtgct gcgagcgggt tggcgtcggt gaacatctgg cgctgcatgc
    20281 gcagcggatg catctgcgtc atcgcgtcgc ccagcggacg cagcggcagc gccgcgagcc
    20341 aggggcggac ccaggtcttg tagagcgagc tgttgatctc cgagaacttg gccaccgtcg
    20401 agaacagctg ttcctcgtcg cggccttccg gattgatcgc ctggatatcg gccatcgtgc
    20461 ggtgctcgaa gcacacgctg tagctgccgg gctccaggtc gtcccagcgc tgcacgttct
    20521 cgccgtcctt cttcaccact ttcatctcgt acaggccggg cgggaggtgg tcgatgacat
    20581 ccagcgaggt gacgatctgg tcgtgctcct tgcgggcgat gtcggcgccc acgaagatgc
    20641 ccaggtgccc gacgctctcg tgcagcacat agacgatgac gcggcccgcg gcggcaatgg
    20701 cacgctcgct gccccaggcg tcgatgatcc agttcagcgc ctgcggcggc ggagtgatgt
    20761 tgtcgcccca agacgcgaag acgaccaccg gcgcggtgat gttgcgcagg tcgacgcgcc
    20821 gttcatcggt catcacctgg cctgacgcca gcttgttgcc gacgaagagg ttctcgacga
    20881 tggtctcgat ctcggggccg ctcatgcgga agtagccgcc ccaccagcgc tcgaactcca
    20941 ggaagcgctc ggactcggtg tccacgttcg cccacagctt gaagtggcgg ctccacagcg
    21001 tgttggccgg gttgagcttc tcgaagttct cgaccaggtg ggcgccgtcg aagcggccac
    21061 cggccaggtc ggacgtcatc gaggccagcc aggccccgcc caggctggca ccgctgtatc
    21121 gcatcgggtt gagcttggag ccgccggccc agtaggacag cggcgagccc acgatcatca
    21181 gcgggccgaa cagctcgggc cgcaccgacg ccagcatcga gatggcccag ccggcctggc
    21241 agttgccgat gaccacgggc ttgccgctcg ccttcgggtg acgcgcgatg acctcctcga
    21301 ggaacatcgc ctcggcgttc atcacgtcga acagcgtctg cccttcttcc ggctgcggat
    21361 ggaaggtcac gaagtacacc ggatgcccgg cgcgcagcgc gacaccgact tcggagtcgc
    21421 tcttgaagcc tccgatgccc ggcccgtgtc cggcacgcgg atcgaccacc accagcggcc
    21481 gtgccgtcgg gtgggcgcgc accccgtcga gcggcaggat gtgcagcagc gcgtagttgc
    21541 aggggcggct cagcttgcgg ccgtcgatca gcagctcgtg cttgaacttc aacagcggcg
    21601 gagcgccttc atcctggtgc gcgacgtagt gattgccgcg ttcgcgcagg gtgtccatga
    21661 acagcacgct gcgctccatg gcgtcgacga cgtactggag gccgggccag gcggcccacg
    21721 cgggtgctgc tgcagagctg gcgggctgtt tgtccatggt gtccccggtt ggttgcctct
    21781 tcggctcttc ggttgtaccg ggtctgcact tgatcggtgg aaaccgccgt gcggcaaaca
    21841 ccggccattt ccgggctttc gaatcgccgc gacgcgggat attcgcgaga ccacatccga
    21901 actcgttccg gggagccaca ccgtgtccat tgccgccctg ttcgacacac cgcaggccct
    21961 gcccaccatt ccgaaggtgg cgcaggaact gatccagagc ttcagccgcg aggacgtggc
    22021 catcgacgag atcgcccgcc acctgtcggc cgatccggtg ctcagcgcca agaccttgcg
    22081 gctcgcgaac tccgcctact tccacgtgtc acgccaggtg gcatcggtcg acgacgccat
    22141 ccgcatgctg ggcttcgtga tgatccgcaa cctggtcatc ggctgcggcg tgaccggtgc
    22201 attcaagtcc gtgcccggcc tcgaccttcc ccagttctgg cggcacagcc tgcacaccgc
    22261 cggcgcctcg cgctggctgg cgcagaccct tgaccgcaac tccgacctcg ccttcaccgt
    22321 cggactggtg catggcgtgg ggcagctcgt gatgcacgcg gcagcgcctg ccgccatgaa
    22381 gccgctggat gccgaatgcc atccgctggc cttcgagcgc accgcgctgg aacaggcccg
    22441 actgggttac caccacggcg aggtgggcgc cgaactggcc gcacgctgga agtttccggc
    22501 cgaggtgagc gacgccctcc gggcggcggc agatccgctg cgtgccggcg cctcttcgga
    22561 gatcggcagc ctggttcaca tcgccgcctg gcgatcgcgc gtggcgctgt tcgatctcga
    22621 tgcggcccag gccgcggcgg gttgtcccgg cgcactgggc cgagcgatcg gcctgccggt
    22681 gacgtggctt gcagccgaag gcacgctggg tttcgaagcc tcgagcggcc tgcctgcgat
    22741 gccaccgctg gccgaactga cgagcggcct ggaagcgatg ctggactgac gcccgccgcc
    22801 ggaacagccc tggcgcaccg gcggccagtc cggttcagac tttctctgcg atgccaagcg
    22861 gcgagcgcgc cgcacgcgac accgtttccg gtggatcgtc cttgcgccgg tgatcggacc
    22921 acacctgccg ccgatggcgc gcacccggaa tgccgttcca caggcccagc atgtggcgcg
    22981 aggcgcgcgt ccacggctcg ccctgcgcca cgaggcgctg catgtagtcg accatggccg
    23041 cctcggccgc gtcgaggtcc aggcccgatg gcgcatcgcc gaaatgccgc tcgtcccagc
    23101 cggcgagcca ccatggccgg tggtacgcct cgcggcccag catcacgccg tcgacatggt
    23161 gcaggtgctc ggcgatctgc tcgtcggagg tcacgccgcc gttcagaacg atggtgagcg
    23221 aagggaagtc gcgcttgagc cggtagacga actcgtagcg cagcggcggc acttcacggt
    23281 tttccttcgg gctcagccct ttcaaccagg cgttgcgcgc gtgcacgatg aacacctcgc
    23341 agcccgcgtc ggacagcgtg ccgacgaagt cgcgcacgaa gtcgtagctt tcaccccggt
    23401 cgatgccgat gcggtgcttc accgtgaccg gaatcgacac cgcatcacgc atcgccttca
    23461 cgccatcggc gaccagctgc ggctcggcca tcaggcaggc gccgaacgcg ccgcgctgca
    23521 cccgttcgct cgggcagccg cagttcaggt tgacctcgtc atagccccag tcctcggcca
    23581 gacgcgcgca atgcgccaga tcggccggct cgctgccccc gagctgcagc gccagcggat
    23641 gctcctcggc attgaactcg aggtgccgct tcacgtcgcc gtggatcagc gcgccggtgg
    23701 tcaccatctc ggtgtacagc cgcgtgcgct tcgtcagcag ccgatggaag tagcggcaat
    23761 gccggtccgt ccagtccatc atcggcgcca cgctcagccg ccaggcgctg acttgttgat
    23821 tcaagatgac cttcttcaga tgagccgtga caggaatggg cttgtgagcc cattttgagg
    23881 gagatcacca cggtctccgc agcacgtcac gggcggcccg gaaagcacaa agcccgccga
    23941 agcgggcttt gatgggacag cagcgggtta ctgcttgcgg cggcgggacg ctgcgatgcc
    24001 tgccagcgcc agccctgcca gtgccaaggt acccggctcg ggcaccgcag tggcattgac
    24061 gccagcgagg ttgtcgaatc cgaatccctc tccggtacca ccgcttatct cgcttaccga
    24121 gcgaaacagc agcgaagagg cggttgagcc gccggttagc acatagccca ggaagtcgcc
    24181 tgcgccatct gtcaggttca tcgcgccgag ctcgatggcg ccatcgaaca cggccaactg
    24241 ggccccttcg gcgggtgtgc caatccaact ctcgccaccg aaggcgccgt tcagctcgga
    24301 aaaggaaatg cggacagtcg tcgcgccgaa gtcggtgaac atcgacgcgt gcgaggtgcc
    24361 gttgttgtca tcgaattgag tcgggggggt ctcgaccaaa ttgcggaaat ccccgctgcc
    24421 ctcttgctgg atcgtcatcc cgttcaaggc cacggcagaa gagcggaacg actggtcggc
    24481 cgcgaaggag gagaagtctt cggtgaacga cgccgcgcca gccgcagcgg accaggcgga
    24541 acggtcggtg aaccacacga tgccagcttg agccgagccc atcacgccga aacccagccc
    24601 ggcaagtacg gacgacaaca cggcaaagcg attcagattc atattttttc cagaataaag
    24661 agggatattt tccaaacaaa gcaatatttg cgccccctgc gccgattctc gcagatgccc
    24721 tgggaacgga gctgccagga acgtctgacg gcaccgattg ggacgcgagt ctgtaaagtt
    24781 tttcgacagc gcagatgcac gctcagcggt gccggcgtcg cctgctcaga ccacccgtcg
    24841 aggaacggat cgaacccacc catgaactgc gccctcgagt tcgcgacctg ttgagcccgc
    24901 tcatgggtca gtggctgaca gcgatgcagt ctgggagcat gatgggttac aaattgagtg
    24961 ttttggaatc tctgcagcag cgctcgtgct gcgggagtcc catgacctgc caacccgtgc
    25021 aagaactgtc cagaaaaccg atacatggct gaagctccat cgaacgagga agaacaccgt
    25081 gccgccgagt tgcgcaggcg cgccgagacc ctgctgctcc aggcagcgaa ctgcccgccc
    25141 cagtcagccg atgagatcca gcgcctgctg catgaactcc gcgtgcatca gatcgagctc
    25201 gagatgcaga acgatgagct caggcgggca cagaacgagc tgcacgcatc cagatcgcgc
    25261 tggatggagc tctatgaatc ggctccggtc ggatacctga cggtcggccc cgcaggggag
    25321 attcggcaat tgaaccggtg tgccgcgaac atgctgggcc gtgcgcgcgg cgagttgctc
    25381 aagcagcctt tcacgaagtt catcttcagc gaggaccagg acaccttcta cctgctgagc
    25441 cagaagctgc tgcttgcgga tgaccagcca tcgtgcgagc tgcgcctggc cgcggccgac
    25501 ggcaagccgg tgtggacgca actggcgacg acagcggcgc acgacccgga cggctcccgg
    25561 gagttgcgca ttgcgctgat cgacatcacg acgcgcaagc tgttcgaggc gagcgtgcag
    25621 tctcagaagg tggccgaggc cgccagccgc gcgaaaagtc tctttctcgc caacatgagc
    25681 cacgagctgc gcacgccgct gaacgcgatc atcggtttcg ccgatctgct caagcgctcc
    25741 gaactgaccc cgaaacaggt cgacaggctc gacaagatca tctcggcggg caagcatctc
    25801 gtcgaagtca tcgactcggt gctcagcctg tcgcgcatcg acgccggaaa gctcgagccc
    25861 gtggatgctg ccgtggacct cccgcggatc attgcggccg tcgtgacgat gctgtcggaa
    25921 caggctgcga cgaaggggct gcacctcgcg gcagaaccgg ttcctgcgac cgcacagctg
    25981 cgcggcgacc cgacgctgct tcagcagacc ctgttgaacc tggtgatgaa cgccgtgaag
    26041 ttcacccctg cgggaaagat cctggtgaga gcctgcatcg aaagcgagac ggccgaccac
    26101 gcgctcgtcc gcttcgaggt ggaagacacc ggcatcggca tcgatccgga agtcctgccc
    26161 cggctgtttt cggatttcga gcaggccgac aattcgagca tccggaccta cggaggaatc
    26221 ggtctcggtc tcgccatcgc gcggcgaatc gcccgcctga tgggaggtga tgcgggcgtg
    26281 acgagcgagc tcggcaaggg cagcgccttc tggttcacgg cgtgtctgcg caagagcgcc
    26341 tcccgacctg accaaccgct ggcgggcccg tccgatgcag ccgaggctgg gttgctggcg
    26401 cgctttgccg ggacgagcgt tctgctggcc gaggacgaac cgctgaaccg cgaggtcatc
    26461 gtgagtctgc tcgccgatct cgaagtggag gtggccgtca acggcgtcga ggcggtccgg
    26521 cttgcggctg cccaccgcta cgacctggtc atcctgggcg tgcagctgcc gcgcatggac
    26581 gggctcgagg ctgcaaggcg gatccgcgaa atcgcaggct acgccagcgt gccgatgatt
    26641 gcgctgacgg ccagcggttg cgacgacgat cgcagtcgca gcatgcaggc ggggatggac
    26701 gcattcctga ccaagcccgt cgattcgagg accctgttca agaccgtatt caattcgctg
    26761 tcgcgacagc gttgaagcaa cccgtgatgc caggagaggt gaacaccatg ccccaagatt
    26821 caagcgagac ccccatgccg cagacgcagc gtgctgctgc agtggttcgt catttgctgt
    26881 acgccggtgc gatcgcgtcg accctgtggc tgggaggctg cgcggtcgag gttcagaaca
    26941 ggcaggccgc ccaggaaatc gccgaaatgt cgaagccccc cggttcggtc tatctgggct
    27001 ggcgggtgtt ccgtgacaag tgctcggcct gtcacggccc ggacgccacc ggcccgtcag
    27061 ccggccccga cctgctgccc agggtggctg aattgggtcc gcgccgattc gtcagcctgg
    27121 tgctcaaccg ctacgactgg aatttcccga ctgcgaaact gcgcggcgag accgctgagc
    27181 gggaaacttt tgtcgacgag atcgtgcagc gcaaggaagg cgctctcacg atgccagcgt
    27241 gggacggcga gcctcgcgtg gccgcccatg tggccgacat ctatgcctac ctgtcggcgc
    27301 gggcacaggg cacgcagggc gtcggccgcc ccgcccagta gcactggtag cacggcaagg
    27361 gacgggcacg cccgggcccg atgaacgcaa cagggcgcac ggcggggcct gtcgagcctc
    27421 gtgaagcgcc gcggcgccga tgtggcgttc agcctgccgc gcgggcgatg ggctcgatga
    27481 ccagcaggac cagttcgccg ctgcccgccg gcatcaggac gcgacgcgcg ctgagccgca
    27541 aggtccgcac gccgagccgc tggaagtcgt gctcgatgac tcgcccctcg aagctgcgct
    27601 cccgcggcag cacgctttcc agcaaatcgt gcatctcggt gaaatcccac cggcgctgcc
    27661 cgacctcgaa caccgactgc ccgaccgtgt cgtccaccga gccgccgaac tcccgataga
    27721 aagcccggtt gcacgacatg acctgaagct ggccatcgag cacgaccagc ggctcgggca
    27781 cggcgtcgac caccgcttct gccagatcgc gcgcctgacg cgtcgcaatg gcatgcacgc
    27841 gctcggtcac gtcggtgaag gtcagcacca cgccgtcgat gacgttgtcg accgtgcgat
    27901 agggctggat gcgggccaga taccaggcgc cggcgggcgt gctgagttcg cgctcgatgg
    27961 gcaccagcga gtcaagcacc gcctgcgcgt cggccaacgg atcgccaccg ctcagatcgc
    28021 tgcggatgtc agccagcggc cgccccaggt cggaggccag cagccgatac accttggtgg
    28081 cctcgcgcgt gaagcgccgg atcagcaggt tgcgatcgag gaagatcgtg ccgacacgga
    28141 tgttgtccag caggttcttc atgtcgtcct gcatgccggc catctgctcg atcttggtct
    28201 gcagttcgga gttgaccgtc accagttcct cgttgacgga ctgaagctct tccttcgagg
    28261 tttccagttc ctcgttggtg gactgcagct cctcgttggt cgactgcaac tcctcgttga
    28321 tggacttgag ttcctcgttg gaggcctgct gctcctcgag cagcgccttc atgctctgct
    28381 tggcgtagtc gagctcgcgt tcgagttcgg cggcccggat cgcgctgcca gacgccctgc
    28441 ccttgcgctt gtggacaaca ggcgcaccgg gcacaggctg ggtccgctcc tggaagctca
    28501 ccagcagcac gttggtgcct gcgccgatgt cgggcagcaa tcgaacgctc aggtcaaccg
    28561 gcagcgtctc cccgtcggtc cggatcgtca cggcctcgtt gatggtggga agtgaatgca
    28621 gcgtggcgcg gttcagggcc tcgcgcaact cgatctgcaa tccctcgcgt gccatctcga
    28681 cgacgttgtg cgtcggctgg cccggagccg gcctcagaaa acgccccgtc tcgccgtgga
    28741 cgtacaggat gttgccctgc aggtcggtga ccactgccgc cggcgtgaac gattgcagca
    28801 gagcgcgcct ggccagttcg ccgacatcga actccttcac cctcagcccc tgctcctgcg
    28861 attccaccgc gctcttggcg accgcccagg acaacccgct cgtcagcacc gatctcgtcg
    28921 atgcgatggt gggcttggcg cggtagaact tccatttgcg gtggagcgga tcgaacagtt
    28981 ccgtgtggtt tccgacgctc tccgaaggcg aaaggaacag gatgccgccg ggcttgagcg
    29041 cgtagtggaa cgccgggatc aggcggttct gcaggtcggg ctcgaggtag atcatcaggt
    29101 tgcggcacga cagcaggtcg agccgcgtga acggcggatc cttgatgacg ctctgcaccg
    29161 cgaacaccac catctcgcga atttccttct tcacccggta gccggcgtct tcctggttga
    29221 agaagcgccg cagccgctcg gcggaaacat cctgcgcgat gttcagcggg tacagcccgg
    29281 cgcgggctgt ggcgatcgca tcgtcgtcga ggtcggtgct gtacagctgc agcttgagct
    29341 ccggatgcgt ggtgtcgatg agctcgcgca ggatcatcgc gatcgagtac gcctcctcgc
    29401 cggtcgcgca gcctgccacc cacacccgca tggtgtaacc ggccggcttg cccaccagca
    29461 gcgccggcag gatctcggac ttcatcaaat cgaacgcttc ggcgtcgcgg aagaagctgg
    29521 tgacgttgat cagcaactcc cggaacagca ccttcagctc ggaagggtgc tccttgaggt
    29581 agcgcgcgta gatctccggg tcctcgatca ggtgctgcgc catgcgccgc tcgatgcgac
    29641 gtccgatcgt gctttgcttg tactgggcaa aatcgtggcc cgttcccgcc cgcagcagcg
    29701 cgaggatctg accgaaaccg cgagccccgg cgccactttc gccaccggca gcaggcgcag
    29761 ccggtgcgac ccgggcgacc ggcaggtgac gtgcgatcgc cagcagcgcc tgcggcatct
    29821 ccgcgacagg cagcacctgg ttgacgtaac cggcgtgcac cgcgctcgct ggcatgccgc
    29881 cgaacttggc ggtggccgga tcctggacca ggcacagccc gcccgcgccg aacaccgccc
    29941 gcatcccgag cgtgccgtcg gtgccggtcc ccgacaggat gatggcgatc gcgttgtctc
    30001 cctggtcctc ggccagtgaa cgcaggaagg cgtcgatcgg catgcgctga ccgcgcggct
    30061 cggccggcac gttcaacaac agcctgccct gcgcgatgac catgtcccgg ttcggcggaa
    30121 tcacgtggac gcggttggct tcgaccacca tgccgtcctg ggcctcgacc acgggaatga
    30181 ccgtgatccg ctgcaggatc tcggtcaaca tgctggcgtg gctcgggtcg aggtgctgga
    30241 ccagcacgaa ccccatgcca ctgtcgggcg tcatggtcct gaagaactgc tcgaaggcct
    30301 cgagcccgcc cgccgacgca ccgagcccga cgatcaacgg cgggcggtct gccgcggttg
    30361 gctgcagggg gatcggcgtg gcgggtgcag cctccgggcc accggcagcc gccgctgaag
    30421 ggtcttcggt cgtcacttcg gtttcctctc gccacaagga cgggatcggt ttcggcgcac
    30481 acgtcgatcg agacgggttg ctgtcctgac gacgcagtcg cgatcagtcc gcgagtcacc
    30541 ctcattgtcg ctgtccggac cgctcccgca acgcaggcga aaccggaaca ggaagaccgg
    30601 ggtgatgctg gcgcagcgcc ctcagcgcac cttgcatcgt ggttcccgtt tgcaatgccg
    30661 tcatcgacca cgccgacgac atgcccggtc agctacctgg ctacggcttg gcaataccga
    30721 gctggacttt cgcgcccgca gccttctcga gcgccgcata gaacgcccgc accgcagcga
    30781 cctctccgcg cacggcccac gtgtcctgat cgcaggcctg gcagtgggcc ccgtacgccg
    30841 cttcgatccc cctgaggcgg ctggcgtcca gcggcgcgat gtccgtcagc tcggtcccgc
    30901 aggtgttgca ggcgatgaca ggcggcttcg acttgccggc ctcgacgatc gcctgcgcga
    30961 ctctggcgtc gcgcgatccc ctgatcttcg cctgtcccaa tgtgcttctc cgcaagttct
    31021 ggccgtgacg ggcgcagtgt aggcgtgcac ccggcgtgct acagcggcgc gctggcctgt
    31081 ggcggtacgg gctgggtcag catcttcttg atcgccttgt cgcgggcgcg ctgctgttcg
    31141 atgcgttcgc tctgcatgta gagcagcggc agcccgagca gcatcagaaa cagcacggcc
    31201 agcagcaatc cggcaaagcc tcgttggtgg tgccggctcg tcatcgccgc gatccccggt
    31261 ttcgtttcga cgcacgcagt gtgcacctgc actgggttgc gcagcgcggg accgtctacg
    31321 tcgaacagcg tagatcactg accaccgccc ccgctcacaa tcgatcggag cgctctgtgg
    31381 cgatcgattg accgtgtgga agcaactgat gaagacgttc ttgtgtatcg gattgatggc
    31441 cttgtcgatg accggttgcg gcgagctctc gtacaagcgg ggcgcttcgg ctcaggatct
    31501 cgaggcgacc aggaaatcat gccagtctgc cggcactgag aaggcggtcg agaagtgtct
    31561 ggaggacaac ggctgggtgg tgaagaagct cgacgacatc gagctgtttg ccactgcgag
    31621 cgtcactgac gacaacagga atctgggcac ttccgagcct gcgaaaggcg ccgacaagcc
    31681 cgcgccagga accgttgcca gatcgcccgc gatgccgggc acgaccgctg cggccggctc
    31741 aggcggcgcc ccggtcgcaa gcccggcgcc cccgcccgct cccccggcca gtccgctgga
    31801 tgtctacaag atcagttcgt ggtggaagct gggcgctggc cgtgaggcga tggaagccga
    31861 cacggctgac tgcgtggccc agctcggcag cgcgcacaag cccgacaacg cgacacagca
    31921 ggtcacgcgg ggatttgtcg tgtgcatgca cggcaagggc tggaaagcgc tgcgcgagaa
    31981 ataggccgac tcggcggggc gcgcgcccgc cgtcaggtgg cttccaggcg ctccatgaag
    32041 cgtgacagcg gttcctgctg ggtcggttcg gccagatagg gctccgtcac ctggtaggtg
    32101 gcaaccgggg tctggccgca gagtcgactc agttcggaga acacgagccg gtcgcccagc
    32161 ccgccctcgg tgcagaagaa ggcgacctgc ttgaagcggg aggcgttctg cagcacgtag
    32221 gagcgcatcg gcgatgccac ccgccacgcc cacaccggcg tgcccaggat caccaggtcg
    32281 taaccgctcg gatccctgcg ggcagcctcg atcggcggct tggcaccgat cagcgaatcc
    32341 cggatgtagc gcagcccttc gcaggcaccg gcgtgcggac gcgcatccct gatttcttcc
    32401 agttcggcac ccgaacgccg ggcgatctcg gacgccacga aacgtgtgtt gcccgtgttg
    32461 gcaaagaagg cgaccaggac ccgcatgcat ttctccgtgt gttggcaggg cagtcacacc
    32521 cccttgaatc ggttatcgac cgccggatcg acaacttgag cccatcccga ccggggcggg
    32581 cgaaccggat tcggtgaccg ctcggtcgtt caggcgcggc ggtgatcgat tggcgcggcc
    32641 atgctgtcca atggcggcat gagcatgtcc tgcaaatctt tcaagagcgt gtcccggtgt
    32701 tccatgcacg cggcctgagc aaggtctatc gcagcggcga agtcgaggtg catgccctgc
    32761 gcgatgtcga tctggacatc gggcggggcg aattcgtcgt gctgctcggc gcgtcgggca
    32821 gcggcaagag cacgctgttg aacatcctgg gcgggctcga cgtgcccact tccggcgaga
    32881 tccggttcgg cgatcagcgc ctcgacggcg ccagcgaggc ggagctgacc gcctaccgtc
    32941 gcgagcacgt gggcttcgtg ttccagttct acaacctgat tccgagcctg acggtgcgcg
    33001 agaacgtcgc gctggtcacc gacatcgcgc tcgacccgat gccggtcgac gaggcgatcg
    33061 atctggtcgg cctgaccccg cgccgcgatc actttccgtc gcagctgtcg ggcggcgaac
    33121 agcagcgggt ggcgatcgca cgcgccatcg tcaagcgccc cgaggtgctg ctgtgcgacg
    33181 agccgaccgg cgcgc
//
LOCUS       DAOORV010000021        32728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1568_length_32728_cov_8.071160, whole genome shotgun sequence.
ACCESSION   DAOORV010000021 DAOORV010000000
VERSION     DAOORV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 32728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_32728_cov_8.071160"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PKV56_04700"
     CDS             <1..986
                     /locus_tag="PKV56_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX67144.1"
                     /translation="AILHLLYARFWTKVMRDLNLVAVDEPFTKLLTQGMVLNHIYSRK
                     TEQGGIEYFWPHDVDNTFGADGKINGATLKGDGSAVTYEGVGTMSKSKNNGVDPQALI
                     DQYGADTARLFVMFASPPEQTLEWNDAGVDGAHRFLKRVWGFGARNAELLKTAGAIPA
                     ELSGAAKALRREVHQVLRQVSYDYERMQYNTVVSGGMKLLNSLEAWKGDDGAAVLREG
                     YGILLRSLYPACPHTTWTLWNELGYAAVHGDLLDAAWPEVDEAALVQDEIELMLQVNG
                     KLRGSITVPAGADKAAIEQAALISPDLLKFADGAPVKRVIVVPGRLVNVVL"
     gene            1000..1548
                     /gene="lptE"
                     /locus_tag="PKV56_04705"
     CDS             1000..1548
                     /gene="lptE"
                     /locus_tag="PKV56_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOX67145.1"
                     /translation="MPARPPTAPAASVGLERRRRAWIAMAAAMAISPLVGCGFELRRA
                     PALNFRTLQLTGFKSNSPFEKELRENINASQTTLVVDSAAQAQVILEALSDRREKSVV
                     ATSSAGQVRELSLRVRFNFRLRTVAGRELIAPTELVLGRDMSYSETTALAKEYEEALL
                     YREMQKDIVIQIMRRLATVPAL"
     gene            1589..2692
                     /gene="holA"
                     /locus_tag="PKV56_04710"
     CDS             1589..2692
                     /gene="holA"
                     /locus_tag="PKV56_04710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOX67146.1"
                     /translation="MQLRLDQLGPHLQKGLRPIYTVWGDEPLLTQEAGDALRAAARRG
                     GHTERQVHTVSGAHFNWSALLGASQAMSLFADKQLIEIRIPSGKPGKDGSEALQRYCQ
                     SLSDDVVTIVQLPRLDRQQQQSAWFSALDAAGVTVRVDPVERKALPLWIAQRLGAQEQ
                     RVQGGEAGQRTLAFFADQVEGNLLAAHQEIQKLALLYPAGELSFEQVESAVLNVARYD
                     VFKLGEAVLAGQVARALRMLDGLRAEGEAAVLVHWTLAEDLRALKRTQDAVKAGRPLP
                     LALREARVWGTKERLFERALPLFTENALAQLLHAAQICDGLVKGLKHPDWPPEPWDGL
                     KRLALMAMQSTSVAPGSTRGRVVVVPPRLALGA"
     gene            complement(2719..3654)
                     /locus_tag="PKV56_04715"
     CDS             complement(2719..3654)
                     /locus_tag="PKV56_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HOX67147.1"
                     /translation="MAILLATDFDAAGLCRWLELLEAALPGEQFVTDRRTVDDAAIDM
                     ALVANPSRGALSGLANLRLIQSLWAGVDKLLGDSSLPPHVPIARMVDPAMNAAMAETA
                     LWAVLSLHRGFYEYAAQQRMRRWHSLVQCRADEVTIAVLGLGQMGQTAALRLAAQGYR
                     VLGWSARPGAKASIEAFAGDDALQPLLARCDIAVNLLPLTPATRGLLCKRTFNAMRRG
                     ASLVNLARGAHVVDEDLLAALDGGHLRHAVLDVFTIEPLPVDHRYWSHPQVTLLPHAA
                     AQTDARSAAAIAATNVRLLRAGGLPQNLADRTRGY"
     gene            complement(3667..4164)
                     /locus_tag="PKV56_04720"
     CDS             complement(3667..4164)
                     /locus_tag="PKV56_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOX67148.1"
                     /translation="MNELDTEQQAGVQAIASALRSRGLRLATAESCTGGLIAAACTSL
                     AGSSDWFERGFVSYSNEAKTELLGVPQELLAAHGAVSEAVVRAMAEGALQRSSAQRAV
                     AVTGIAGPGGATQDKPVGTVWLAVARSDGDTTALLLQLAGDRAAVRHQTVTRALRALL
                     ASLRS"
     gene            complement(4184..4741)
                     /locus_tag="PKV56_04725"
     CDS             complement(4184..4741)
                     /locus_tag="PKV56_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOX67149.1"
                     /translation="MNESSLAAPALSQTARRANVRLLLSHPAHLVALGFGSGLSPIAP
                     GTVGTLWAWMAFLVLHPHLSTPYMAGLLAASFFVGWWACTVTARSMAVADPGAIVWDE
                     VLAFWLVLWLVMPSGLLAQAVAFALFRYFDAAKPGPVAWADRVFKGRPGQPIGWAQGF
                     GIIFDDLVAALCTLLVFAGWRWLWP"
     gene            complement(4738..5727)
                     /gene="thiL"
                     /locus_tag="PKV56_04730"
     CDS             complement(4738..5727)
                     /gene="thiL"
                     /locus_tag="PKV56_04730"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOX67150.1"
                     /translation="MSLGEFDLITRYFDRPARRATLGIGDDCALFAPAPGMQIATSCD
                     MLVEGRHFLSTVAPERLGHKALAVNLSDLAACGAEPLAFTLALALPRSDEAFLDGFSR
                     GLLALADAHRCELIGGDTTQGPLNICISVFGQVPAAATGTGALLRAGARSGDDLYVSG
                     SLGDARLALEVFRGTLSLDGREFDAVRDAMELPQPRVALGLALRGLASSAIDVSDGLL
                     GDLAHILERSGVDAIVEIDALPRSVVLRGQPMALQRLCMLSGGDDYELLFTAAPDRAS
                     QVVAAAASAGVAVSRIGRIVPRTNAAAGPRAELIDAGGQSVVHRHASFDHFRT"
     gene            5986..8283
                     /locus_tag="PKV56_04735"
     CDS             5986..8283
                     /locus_tag="PKV56_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOX67151.1"
                     /translation="MTTSNDSKKAELRRAALEYHEFPTPGKIAIAPTKQLVNQRDLAL
                     AYSPGVAAACEEIVADPANAFKYTARGNLVAVITNGTAVLGLGDIGPLAAKPVMEGKA
                     VLFKKFAGIDVFDIELAERDLDKLIDTIAALEPTFGGINLEDIKAPDCFYVERALRKR
                     MKIPVFHDDQHGTAIVVGAALLNALKVTGKDIKKVKLVTSGAGAAALACLSLLVKLGL
                     PRENIWVTDLAGVVYEGRTELMDADKIVFAQKTAHRSLADVMRGADVFLGLSAGGVLK
                     KEVVATMNPQPVIFALANPTPEILPEEIKAVRDDAIIATGRTDYPNQVNNVLCFPYIF
                     RGALDSGATTITVEMEIAAVHAIADLAQAEQSEVVAAAYAGANLSFGPEYLIPKPFDP
                     RLMIRIAAAVAEAAAASGVAARPVLDMDAYRERLQSFVYASGTTMKPIFNLAKRALQK
                     RVAYAEGEDERVLRAAQIVIDEGLARPTLIGRPEVIGARIEKFGLRLEAGRDYDVVNT
                     DNDHRYRDYWQTYHQLTARKGVTAQFAKIEMRRRLTLIGAMLLYKGDVDGMLCGTWGN
                     SALHLQYIEQVIGLREGARTFACMNGLLLPGRQVMLVDTHINYDPTAEQLAEITVMAA
                     EVMVRLGLRPKAALLSHSNFGSSNHPSAVKMRETLTLLRQDAPWLEVDGEMHGDTALD
                     ADYRRGLMPMSTLSGEANLLVLPNIDAANISYNLLKTAAGGGIAIGPVLLGAAKPVHI
                     LTASATVRRIVNMTALTVAEANATR"
     gene            8939..9334
                     /locus_tag="PKV56_04740"
     CDS             8939..9334
                     /locus_tag="PKV56_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOX67152.1"
                     /translation="MLLQTVRSNIVQSIRAYRVEDLMLAAQNADQHFLYANLTSAQAK
                     QDVLDTIAESFLFPVHFGKNLDALYDCMTDLVHKAGSQPGFVVVLEQLPDNPRFDREA
                     REQLLDVFRDAADFWAERKVPFRCFYSFQ"
     gene            complement(9569..10327)
                     /gene="rsmA"
                     /locus_tag="PKV56_04745"
     CDS             complement(9569..10327)
                     /gene="rsmA"
                     /locus_tag="PKV56_04745"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOX67153.1"
                     /translation="MAHTARKRFGQHFLTDRVAIDSIVDAIAPHPGETLIEIGPGLGA
                     MTFPLLERCEHLTVIELDRDLAARLRRQPALTVIEADVLNVDFGELHRAAGQRLRVVG
                     NLPYNISTPILFHLLPVAAQVLDQHFMLQKEVVERMAAVPGSKVYGRLSVMLQWRYAI
                     ESVLDVEPGSFDPPPQVRSAVVRMLPLPAREDVDATLLGELVTVAFSQRRKMLRNTLG
                     RWLDERGFAGDFDLQRRAEEVAVSEYLSLCAAVR"
     gene            complement(10382..11710)
                     /locus_tag="PKV56_04750"
     CDS             complement(10382..11710)
                     /locus_tag="PKV56_04750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX67154.1"
                     /translation="MRSIVVAAVAVGALAGQVPQSFAQAPRAAASSASLRSGDFILAV
                     VNQELVTAREVEQRLARVRENAARTKTTLPPETELRRQLLDLLIDERAQISHARESGQ
                     RIDDSELDRAVGTVASQNQVTLAQLRQKLAEDGVDYARFRKNLRDQILVERVRDREVQ
                     ARIRIPDTEIEALIAKQRAESSNRAEYNIAQILVTVPENASEELVAERKARAESAMAR
                     VKSGESFATVALEISEDANKAQGSSLGLRTADRLPDVFVERVRGLKSGQIADALLRTG
                     AGFHVLKLVERREGQAFSITETRSRHILLKITAQLDQAAAVRRLNDFKRRVQDGSSSF
                     EQLAKDNSEDGSASQGGELGWTSPGTFVPEFEEVMNRLPVGGISDPVVSRFGVHLIKV
                     DERRQATLDAKQERDQARNALREKKYEEAYAEWTREVRARAYVELRDPPQ"
     gene            complement(11715..13985)
                     /gene="lptD"
                     /locus_tag="PKV56_04755"
     CDS             complement(11715..13985)
                     /gene="lptD"
                     /locus_tag="PKV56_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOX67155.1"
                     /translation="MPSLVTEGGVLLKIAPALTPLPRGEAGKQLPLSVQAQTVRGRPD
                     LDTIAEGEAVLRRGDLTLRADLLSYDHVEDLALARGNVQINQEGNRYTGPELQLKVQR
                     FEGYFINPTYFFGRTAAGGKAERIDFIDSQRTVATKATYSSCPADGSGDPDWLLTSDR
                     VKMDFNNNEGFAEGAVLRFLGVPILWAPALSFPLTDERKSGWLPPSVAIDSRSGVHLE
                     VPWYWNIAPNRDATFTPSASIRRGVGLGSEFRYLEENYHGELNLNLLPHDREAGRTRY
                     AFGAKHEAAWADDTRLSVRMQRVSDDDYWKDFRRDLNTTTPRLLLSDIQATRPFGEWT
                     TYARAQRWQVLQNADSLIDAPYERLPQIGARTLQPVGEGFEVGFEGEFNRFGNPIGTV
                     NNASRLTGLRAHAIASLSRPWVTPGWTLTPKVSVNAASYSLDSPMSDGRKQASRVIPT
                     FSLDSAWVLERDSSWFGKDMRQTLEPRLLYVNTPYRKQSILPNFDSAPRDFNFETIFT
                     ENGFSGVDRVSDSHALTAGVTTRLLDPMNGGEAMRLGVVQRYLFSDQRVTPEGTKLTQ
                     RISDLLLLGSTNVIPDWSLTATVQYSPQLGTTVRSIVGAAYSPKPFHTVSAVFRETRN
                     LSEQLELGWQWPVFGRTPDAPNLSGDSRGDPASCKGSLYTVGRVNYSTRDSRIVDSIL
                     GFEYDAGCWITRVVAERLSTGRAEATTRLLIQLELVGLSRLGSNPLQVLKDNVPGYRL
                     LRDAPASSQNTFSFYD"
     gene            14336..15442
                     /locus_tag="PKV56_04760"
     CDS             14336..15442
                     /locus_tag="PKV56_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOX67156.1"
                     /translation="MTPVPPSTAPDVRPGVVWADPARRADFQRWITTVAPAHRLHTET
                     LRPASSDASFRRYLRIDGEAGSFIVMDAPPAHEDVRPFVAVASLARKAQLNVPQVLAQ
                     DVEHGFLLLDDLGTQLYLGELQAAVEAADNASVERLMRSAMSALVVWQQRVDASALPA
                     YDDALLRRELALFPEWCVKREFGVSWNADEQATWATTCDLLVRSALDQPTVAVHRDYM
                     PRNLMVCDGNTVADGVLANPGILDFQDAVRGPISYDVASMLRDAFISWDEEQELDWAI
                     RYWQQARKVGLPVPADFGEFWKPLEWMGLQRHLKVLGIFCRLKHRDGKPAYSADLPRF
                     FRYAHKVATRYEGLGPLARLLEPLMGATRVDAFY"
     gene            complement(15461..16939)
                     /locus_tag="PKV56_04765"
     CDS             complement(15461..16939)
                     /locus_tag="PKV56_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HOX67157.1"
                     /translation="METETDSTLEPANRAPLAAGTIVDGWQLEEKLHTGGMAHLWRVT
                     RVSGGSGTEPASNDVPADLPLIMKVPRIMGGEDPASIVGFEVEQMILPALQGPHVPRF
                     VARGDFSREPYIVMERIDGPSLRARLDEAPLAIDEVIEIGSRVATALHELHRQHLVHL
                     DVKPSNIVFRADGTAVMVDFGLSHHDRLPDLLDEEFSLPMGTGPYISPEQVQYVRDDP
                     RSDLFALGVMLYHFTTGQRPFGSPVTVRGLRRRLYVDPIPPRALRADCPPWLQEVILK
                     CLEVKAERRWQSGAQLALALQDPTQVPLTARAQRAHVSNAFQRLKRWFFAIGAEPQTS
                     PLEGVTSQVMRSPIVMAAIDVDQASGELLDRLRETVRRIVVTEPGARLACVSVMKTAR
                     IGMDELVDAAGQSVHVKQLVGLKHWARPISKQLNLEDGRLTFHVLEAPDAATAIVEFA
                     ARNQVDQIVMGARGNSTLRRYLGSVSSQVVAQADCTVTVVRA"
     gene            complement(16929..17672)
                     /locus_tag="PKV56_04770"
     CDS             complement(16929..17672)
                     /locus_tag="PKV56_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOX67158.1"
                     /translation="MKIAVITDLHANREAVQAVLEHAAAQGAQHHAFVGDFVGYGGDP
                     AWVVDCVRQHVEGGATAVMGNHDAAVAQGARRTMRAEARFVIDWTRERLDAGQLDFLD
                     RLPMTHERGDLLFVHANAYAPPEWGYVEGKSEAVRSLLATRQRITFCGHMHEPMLYHL
                     SATGKSGSFVPVAGEAIPLLASRRWLVIPGSAGQPRDGNPAACYALYDEDAAELTYWR
                     VPYDHEAAMAKIRAAGLPQSLAARLADGN"
     gene            17653..18480
                     /locus_tag="PKV56_04775"
     CDS             17653..18480
                     /locus_tag="PKV56_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOX67159.1"
                     /translation="MITAIFMAQVFSTALESARRRSFRMNLPASTLTLLLTVAFAVAL
                     MACERTRPNVNYPSDWRWVGRAAGFGIAGVLLTMLLGAWIESHASVFSFLRRENSLTR
                     LPDWIQGFAGYLCVTFIVYWWHRFRHQSDRLWTLFHQIHHSTYRLEVATAFYAHPTDF
                     LATTVIVSAVAYGLLGYGLDAAAWTAFWVGIFELWEHTNIRTPRWLGYIIVRPEMHRV
                     HHEKDQHRNNYGLPVWDMLFGTYENSMRSVVCGFTREQEEQVLPMLLFKHADRQLGP"
     gene            18775..19527
                     /locus_tag="PKV56_04780"
     CDS             18775..19527
                     /locus_tag="PKV56_04780"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HOX67160.1"
                     /translation="MQFYARALATALCLSAAATGIAQAAVITQTGNFSLASAELKRRD
                     SGNSFNSTVVSVFSDPQSLSQSMSFDLFDSSLGILTGVTFWLSNPLGTDILTLSEGIA
                     QDNGLTQAAGSVTRTLTGPSGPSISLVSAFLGTACGFSDTCMTDTLSHSPLATDVGGE
                     SLVPLAGFIGQGGFDITAGLALAAPTISYIGGIPDCDSTEATLTSSWAGTVRLEYAYN
                     AAPPSNEVPEPGSLYLAGAALLAAIGLRRRLA"
     gene            complement(19566..21686)
                     /locus_tag="PKV56_04785"
     CDS             complement(19566..21686)
                     /locus_tag="PKV56_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HOX67161.1"
                     /translation="MLVILALVAASHVRWQQKSAWERDAAHYQSDGIPMMSTVDAYYA
                     LRLARQWAAGQFEPGAPATPRHFTRPPKGQPDAWAAQWEPRSLPLLSRLIASVAAWSG
                     MGIDRTAWMLPPLLSSLFMVPLFWVFWRLGVPAAGVMAGLVGTFCLEYLARTGVGWSD
                     TDQLNLLFPWLLVALFLQMGSDRQVWRLWGWAAVAGLVNLVYSQWYWKPGLALGLAAV
                     LALHLVLAGTPWRRWLPAVALFVLCSGPQQFVYALASLSDFGAHYLWPDRAVLQLPPA
                     LRFTTVWATISEVQSLNVGDTLERVLPHAGWAALGGVFFLAWALPRWRALLPLLPWVL
                     LGAMAMFGSRRFVLYLAPFVGAGWGIAIAWLVGLTLRPAEARGRPMEPPRPASAGGAP
                     LAVLRAKLAGREAQAVVASLAAVLVFVVWFRPAADRLQAPPPAIPAPVYQDLKALATQ
                     VPADTRLWTWWDLGFAIVDTTGLGVYHDGAVQFTPQTNMVAVSLARPDPALLHALVRF
                     VDHAGNSGIVAQAAHAQDLDGLLDRLRQAAPEQMEVPVLVVFTPDMLPKFSSIESLAT
                     PMTVAKGRHRPFGIVQLACNSLSAELLRCDGVTVDLRAALVHRTGLPAGGASAPRPLR
                     RVVLLADGQVSQEHLLNQGPGLTVQLLTDRGQVKAVYLLDETAYASNLNQMYLLGRYD
                     ARFFEPIYNRLLGLSAYRVLPVAP"
     gene            21943..22164
                     /locus_tag="PKV56_04790"
     CDS             21943..22164
                     /locus_tag="PKV56_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67162.1"
                     /translation="MTKPSAVIAQAQEALDTSIERRRAILKRLGQTSVVAAAVAAPVA
                     ASAGVLSRWNLDPSKRTGSSGNLKLGVSS"
     gene            22283..22561
                     /locus_tag="PKV56_04795"
     CDS             22283..22561
                     /locus_tag="PKV56_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67163.1"
                     /translation="MLFSLNPQIDLIWRDWGDESVAMEAASGRVFLFDPLSSALLACF
                     ESGPQQVQAAVDSLLADLGSPTDSRLQEFLRAALDRFVALGWLLPGDA"
     gene            22562..23485
                     /locus_tag="PKV56_04800"
     CDS             22562..23485
                     /locus_tag="PKV56_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HOX67164.1"
                     /translation="MKLGQLGLQALGKRLRGPGLAWRSGPFSIRLRTDLVAVRDGLHF
                     LYADHPLVPGGDFCDGDITLRQPRAAWGWRRPMVQAHVDYQPIFEPVPVAHALPLVEW
                     LLNWHVTAHGHHFLTVHAAALERDGKALLLPAPSGSGKSTLCAALVHRGWRLLSDEYA
                     LIDFATGLVHPLARPVSLKNQSIEVLRAFAPEAVFSAPAHGTPKGTLAQMKAPAAHVQ
                     RMDEPACPVWIVFPQWSPGATLLLQARPKARALLELTRQTVNLDLAGHDGFARLAELV
                     TASECLDLRYDMLADAVAAIDAMAQAIDHAG"
     gene            23475..24560
                     /locus_tag="PKV56_04805"
     CDS             23475..24560
                     /locus_tag="PKV56_04805"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX67165.1"
                     /translation="MPGDLLTGLWTSNTAPQWHAADWERLFSQARRARLLGRVAWLLQ
                     TRGWTDCAPPEARVHLHNALRAVARQRQAAHWEVDCVRRAIGHLPTPVVLLKGAAYVL
                     ADLPPAKSRRFGDIDILVAAPALGAVEAALAAHGWAAMPLDPYDDRYYRQWSHELPPL
                     RHEERGTMLDVHHCITQPGSRFAVDAQRLLADARPLQGQPGLMVLSPADMVLHAAAHL
                     MQEGDLSRGLRDLLDMHDLLTCFGAQPDFWPALVDRAATLGLCEPLLDVLTQAQCKLG
                     LVVPQPAWQQLQQQSARPLRHRLTAAMLAVALRPNHPDADCRSTRLVRAWFYLRAHRI
                     RMPLHRTLPHLLHKAWLRLRHRPPGLT"
     gene            complement(24767..26176)
                     /gene="mnmE"
                     /locus_tag="PKV56_04810"
     CDS             complement(24767..26176)
                     /gene="mnmE"
                     /locus_tag="PKV56_04810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOX67166.1"
                     /translation="MLPRHHDPIVAIATASGRAAVGIIRVSGRDLRPLIAVLCRRSLA
                     PRHATLLPFLDANGGPIDHGLAIHFPAPHSYTGEDVLELQAHGGPVVLQLLLARCIEA
                     ASTLDPSSGLPVLPGLRLAMPGEFTERAYLNDKLDLAQAEAVSDLIDASTEAAARSAS
                     RSLSGGFSAEIRELDARLMRLRMLVEATLDFPEEEIDFLQQADAQGQLSSISEKLHGI
                     EARTRQGALLRDGITVVIAGQPNVGKSSLLNALAGAELAIVTPIPGTTRDKVTQTIQI
                     EGVPVHVIDTAGLRETDDTIEQMGIARSWTEIEQADALVFLHDLTRLGQPDHDAAESA
                     IRQRLPRALVEAGRVLEVYNKADLPHGCSAGPSELRLSATTGLGLDTLRHRLLELAGW
                     LDHPEGVYAARARHVQALQQTRAHVDEATRQWSGVAPALDLLAEELRLAQHALGAIVG
                     QSTPDELLGEIFSRFCIGK"
     gene            complement(26238..27947)
                     /gene="yidC"
                     /locus_tag="PKV56_04815"
     CDS             complement(26238..27947)
                     /gene="yidC"
                     /locus_tag="PKV56_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOX67167.1"
                     /translation="MTEMRRTLLWVVFSMSLVLLWDAWNKHTGQPSLFGTPQVTQPAA
                     SGPMPAGSAAVPAAAALPAGTPSAMPAAPVADASTAAPLQSQKVDITTDLVKATVDTL
                     GGSMVRLELLKQADHADRDRNVVLFDESSSRLYKAQTGLIAAQGGAAMPNHLTVFNLI
                     PGDLTLKQDQDRLEVRLESPDVGGIKLIKTYVFQRGSYVVAVKHDVVNVSTTPVTPQV
                     YYQFVRDGNAPEGESSFYFTFTGPAVYTDAAKFRKVEFKDIEKRTGTDKPDHDTAADN
                     GWVAMVQHYFAGAWLLGATADEKRPREFFTRKVDTNQYSVGMIVPLGTLAPGATQTLD
                     ARLFAGPQEEDKLSQLAPGLELVKDYGWLTVLSKPLFWLLTQLHKLIGNWGWAIVGLV
                     FLLKIAFYWLNATAYRSMAKMKAVNPRVMEMRERLKDKPQQMQQEMMRIYREEKVNPL
                     GGCLPIFVQMPFFIALYWVLLASVEMRNAPWIGWITDLSAKDPYFILPILMTVTSLLQ
                     TWLNPTPADPMQAKMMWIMPLVFSIMFFVFPAGLVLYWLTNNILSIAQQYMINKQLGV
                     LGK"
     gene            complement(27944..28174)
                     /gene="yidD"
                     /locus_tag="PKV56_04820"
     CDS             complement(27944..28174)
                     /gene="yidD"
                     /locus_tag="PKV56_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOX67168.1"
                     /translation="MAQRALVGTVRLYRLLLSPWLGSACRFEPTCSVYAIGALEAHGA
                     AVGSYLAAKRVLRCHPWCPGGHDPVPNKKLFS"
     gene            complement(28192..28623)
                     /locus_tag="PKV56_04825"
     CDS             complement(28192..28623)
                     /locus_tag="PKV56_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOX67169.1"
                     /translation="MIDVITQAQDFTQVLNAPCQVRSPHFAVHHLPRAPGTGRVELST
                     APAPTSCPPVDESALTGCWLGLVVPKRHAKRAVTRTLLKRRIRSAVSNVCGLPAGMWV
                     VRLRTPFARTEFVSAASDRLSAVVTDELAALMRAAVARATR"
     gene            complement(28679..28813)
                     /gene="rpmH"
                     /locus_tag="PKV56_04830"
     CDS             complement(28679..28813)
                     /gene="rpmH"
                     /locus_tag="PKV56_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOX67170.1"
                     /translation="MKRTYQASKTRRARTHGFLVRMKTRGGRAIINARRAKGRKRLAV
                     "
     gene            29118..30560
                     /gene="dnaA"
                     /locus_tag="PKV56_04835"
     CDS             29118..30560
                     /gene="dnaA"
                     /locus_tag="PKV56_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOX67171.1"
                     /translation="MTEDLWQRGCERLAAELPEQQFNTWIRPLPAAVVSETVDGATAC
                     LLVPNRFKLDWIRNQYGGRIEAVLSDLAGKPVKLEINLAPRDAQPRAAALVTPATVAS
                     RAVDSRNATRSHDPIDPYSASTVEVNSSSTPSAQGNGLNRHRLNLSLTFSNLVPGRAN
                     QMARTAALHVAGAPGQMYNPLFIYGGVGLGKTHLMNAVGNALLADRPDARVLYLHAEQ
                     FITDVVKNYQRKTFDELKAKYHSLDLLLIDDVQFFAGKERTQEEFFNAFEALLAKRAH
                     IIMTSDTYPKGLVDIDERLTSRFDAGLTVAIEPPELEMRVAILMKKAEVEGTTMPEDV
                     AFFVAKNVRANVRELEGALRKILAYSRFSQKEINIALARDALKDLLSIQNRQVGVENI
                     QKTVADFYKIKIADMYSKKRPASIARPRQIAMYLAKEMTQKSLPEIGELFGGRDHTTV
                     LHAVRKISAERQKNTELNQQLHVLEQTLKG"
     gene            30802..31920
                     /gene="dnaN"
                     /locus_tag="PKV56_04840"
     CDS             30802..31920
                     /gene="dnaN"
                     /locus_tag="PKV56_04840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOX67172.1"
                     /translation="MIVLKTAQEKLLGALQSVSGIVERRHTLPILANVLLRKNGGEIE
                     FTTSDLEIQVRTRSTFDGDPSAFATTVGARKLIDILRSLPADQIVTLSANQSKLTLQG
                     GKSRFTLQTMPSDDFPLVQEAADFGPVFKVPQSTLKGLIDQVHFAMAVHDIRYYLNGI
                     LFVAEGKSLTLVATDGHRLALAQATLDAEIPKQEVILPRKTVLELQRLLKSDKGGDDN
                     LIEMRFAGNQAKFSFSGMEFVTKLVEGKFPDYNRVIPKNHKNSVTLGRATLLASLQRA
                     AILTSEKFKGVRVNIEPGTLRIASSNAEQEEAKEEIEIDYGGDTIEVGFNVTYLMDAL
                     SNISQDMVKIELQDGNASALITVPEQSGFKYVVMPMRI"
     gene            32041..>32728
                     /locus_tag="PKV56_04845"
     CDS             32041..>32728
                     /locus_tag="PKV56_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67173.1"
                     /translation="MTDATLPNNPKKTTSSGVTQREVPTAEGAAASTGNEASAAYGEG
                     SIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSD
                     NSISVIDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGV
                     SCVNALSKWLRLTVRREGKAHYMEFRKGFPQDRILEQRDGFEVSPMKITGETEKRGTE
                     VHFLPDDEIFE"
BASE COUNT         6004 a        10780 c        10531 g         5413 t
ORIGIN      
        1 acgccatcct gcacctgctg tatgcgcgct tctggaccaa ggtgatgcgc gacctcaatc
       61 tggtggccgt ggacgagccc ttcacgaagc tgctgacgca gggcatggtg ctgaaccaca
      121 tctactcgcg caagacggag cagggcggca tcgagtattt ctggccgcat gacgtcgaca
      181 acaccttcgg tgccgacggc aagatcaacg gcgccacgct caagggcgac ggctcggcag
      241 tgacctacga aggcgtgggc acgatgagca agagcaagaa caacggtgtc gacccacagg
      301 cgctgattga ccagtacggc gccgacacgg cgcggctgtt cgtgatgttc gcttcaccgc
      361 cggaacagac cctggaatgg aatgatgccg gtgtcgacgg cgcgcaccgc ttccttaagc
      421 gtgtctgggg tttcggcgcc aggaacgccg agctcctgaa gaccgccggc gcgatcccgg
      481 ccgaactgtc gggcgcggcc aaggcgctgc gccgcgaagt tcatcaggta ctgcgccagg
      541 tcagctacga ctacgaacgg atgcaataca acaccgtggt ctcgggcggc atgaaactgc
      601 tgaattcact cgaggcgtgg aagggcgacg acggcgcggc ggtgctgcgc gagggttacg
      661 gcatcctgct gcgatcgctc tatcccgcct gcccgcacac cacctggaca ctttggaacg
      721 aattgggcta cgcggccgtg catggcgacc tgctcgacgc ggcctggccc gaagtcgacg
      781 aggctgcgct ggtgcaggac gagatcgagc tgatgcttca ggtcaacggc aagctgcgcg
      841 gttcgatcac cgtgcccgct ggtgccgaca aggcagcgat cgagcaggcc gcgctgatca
      901 gcccggactt gttgaagttc gccgacggcg cgccggtcaa gcgcgtgatc gtggtgcccg
      961 gccgcctggt caatgtggtg ctctgaaagg cccgaaccga tgcccgcccg cccgcccacc
     1021 gcacccgccg ccagcgtcgg cctcgaacgt cggcgccgcg cctggatcgc catggcagcg
     1081 gcgatggcga tatcgccact cgtcggttgc ggattcgagt tgcgccgcgc gccggcgctg
     1141 aacttccgca cattgcagct caccggcttc aagtccaact cgccattcga gaaggagttg
     1201 cgcgagaaca tcaacgccag ccagaccacg ctggtcgtcg attccgccgc gcaagcgcag
     1261 gtgatcctcg aggcgctgtc cgaccggcgc gagaagagcg tcgtcgcaac gagttcggcc
     1321 ggtcaggtgc gtgaattgag cctgcgcgtg cgcttcaatt ttcgattgcg cacggtggca
     1381 ggacgcgagt tgattgcgcc gaccgagctg gtgctcggcc gcgacatgag ctacagcgaa
     1441 acgaccgcac tcgccaagga atacgaagag gcgctgctgt accgcgagat gcagaaagac
     1501 atcgtcatcc agatcatgcg gcgactggcc accgtgcctg cgctctgagc cccaaacccc
     1561 ccggcccctt cacgcacccg ctgtcacgat gcaactgcgc ctcgaccagc tcggcccgca
     1621 cctgcagaag ggcctgcggc cgatctacac cgtgtggggc gacgaaccgc tgctgacgca
     1681 ggaagcggga gacgcgctgc gtgcagcggc gcgccggggc gggcacaccg agcggcaggt
     1741 gcacaccgtc tcgggcgcgc atttcaactg gagcgccctg ctcggtgcgt cccaggcgat
     1801 gagcctgttc gccgacaagc agctcatcga gatccgcatt ccttcaggca agcccggcaa
     1861 ggacggatcc gaggccctgc agcgctactg ccagtcgctg agcgatgacg tcgtgacgat
     1921 cgtgcagctg cccagactcg accgccagca gcagcagagc gcctggttca gcgcacttga
     1981 cgccgctggc gtgactgtgc gcgtcgatcc ggtcgaacga aaggcgctgc cgctgtggat
     2041 cgcccaaagg ctgggtgcgc aggagcaacg ggtgcagggc ggggaggcgg gtcagcgcac
     2101 gctggccttc tttgccgacc aggtcgaggg caacctgctc gccgcgcacc aggaaataca
     2161 gaagctcgcg ctgctgtatc cggccggcga gctgagcttc gagcaggtcg agtcggcggt
     2221 gttgaacgtg gctcgctacg acgtgttcaa gctcggcgaa gcggtgcttg caggtcaggt
     2281 cgcccgggcg ctgcgcatgc tcgatggatt gcgtgccgag ggcgaggcag cggtgctggt
     2341 gcactggacg ctggccgagg atctgcgtgc tctgaagcgc acccaggacg ccgtcaaagc
     2401 cggccgaccg ctgccgctgg cgctgcgcga ggcacgggtc tggggcacca aggagcgcct
     2461 gttcgagcgg gcgctgccgc tgttcaccga aaacgcgctg gcgcagctgc tgcacgctgc
     2521 gcagatctgc gacggtctgg tcaaggggtt gaagcacccg gactggccgc cggagccgtg
     2581 ggacggcttg aagcggctgg cgctgatggc gatgcagagc acctcggtcg cgcccggctc
     2641 gacccgtggt cgggtggtcg tggtgccgcc tcggctggcg ctgggcgcct gaaagcggcc
     2701 agccgctggc ttgcgccctc aatagccccg ggtccgatcc gccaggttct gcggtagtcc
     2761 gccagcccgc agcaatcgca cattggtggc ggcgatggcg gcggcgctgc gcgcatcggt
     2821 ctgcgcagcg gcgtgcggca gcagcgtcac ctgcgggtgt gaccagtaac ggtggtccac
     2881 agggagcggc tcgatcgtga agacgtcgag caccgcatga cgcaggtgac cgccgtcgag
     2941 tgccgcgagc agatcctcat cgacgacatg cgcgccgcga gccaggttca ccaggctcgc
     3001 cccgcggcgc atggcgttga aggtgcgttt gcaaagcagg ccgcgcgtcg cgggcgtcag
     3061 cggcagcagg ttgacagcga tgtcgcagcg cgccagcagc ggctgcagcg catcgtctcc
     3121 ggcgaaggcc tcgatcgagg ccttcgcacc cggccgggca ctccatccga ggacccggta
     3181 gccctgtgcc gccagccgaa gcgcagccgt ctggcccatc tgaccaaggc ccagcaccgc
     3241 aatggtgacc tcgtcggcac gacattggac cagagagtgc cagcgccgca tcctctgctg
     3301 agcggcgtac tcgtagaagc cgcggtgcag gctgagcacc gcccacagcg ccgtctcggc
     3361 catcgcggca ttcatcgccg gatcgaccat gcgcgcgatc ggtacgtggg gcggcaggct
     3421 ggagtcgcca agcagcttgt cgacgcctgc ccacagcgac tggatcaggc gcaggttcgc
     3481 cagcccgctg agtgcaccac gcgaagggtt ggcgacgagg gccatgtcga tggctgcatc
     3541 gtccaccgtc cggcggtcag tcacgaactg ctcgccgggc agcgccgcct ccagcaattc
     3601 gagccagcgg cacagcccgg cagcgtcgaa gtcggtcgcg agcaggatcg ccatgcgccg
     3661 tgcggttcag gaacgcagcg aagcgagaag cgcccgcagc gcccgcgtca ccgtctgatg
     3721 ccggactgcg gcgcgatcgc cggcaagctg cagcagcagc gccgtggtgt ctccatccga
     3781 ccgcgcgacc gcaagccaga ccgtgccgac aggcttgtcc tgcgttgcgc cgcccggccc
     3841 ggcgatgccg gtcacggcca cggcccgctg cgcggaggat cgctgaagcg cgccctcggc
     3901 cattgcacgc acgacggcct cactgacagc gccatgcgcg gccagcaact cttgcggcac
     3961 gccgagcagc tcggtcttgg cttcgttcga gtagctgacg aagcctcgct cgaaccagtc
     4021 gctcgaacca gccagtgagg tgcaagccgc agcgatcagg ccgccggtgc aggactcggc
     4081 cgtcgccagg cgcaaaccgc gcgaacgcag cgcgctggca atcgcctgaa cgccggcctg
     4141 ttgttcggta tcgagttcgt tcatgctgca ctccgaattc ggatcagggc cagagccagc
     4201 gccagcccgc gaaaacgagc aaggtgcaca gggctgcgac caagtcatcg aagatgattc
     4261 cgaagccctg tgcccacccg atgggttggc cggggcggcc cttgaagact cgatcggccc
     4321 aggccaccgg gcccggcttg gcggcgtcga aatagcggaa cagcgcaaag gcaacggcct
     4381 gcgccaacag tcccgagggc atcaccagcc aaagcaccag ccagaacgca agcacctcgt
     4441 cccagacgat ggcgcccggg tcggcaaccg ccatcgatcg ggccgtgacg gtgcatgccc
     4501 accagccgac gaagaaactg gcggccagca gccccgccat gtaaggcgtg gagaggtgcg
     4561 ggtgcaagac caggaaggcc atccatgccc acaacgtacc gacggtaccc ggtgcaatcg
     4621 gcgacagccc actgccgaag ccgagcgcaa cgaggtgcgc cgggtggctc agcagcagcc
     4681 gtacgttcgc ccggcgagct gtttgggaca acgcaggcgc ggcgagagag ctttcgttca
     4741 tgtgcggaag tggtcaaacg acgcgtgccg atgcaccacg gattggcccc cggcatcgat
     4801 gagttcagca cgggggccgg cggccgcgtt ggtgcgcggc acgatgcggc cgatgcggct
     4861 gacggcgacg ccggctgatg ccgcagctgc caccacctgc gacgcccggt ccggagcggc
     4921 ggtgaacagc agttcgtagt cgtcgccacc cgacagcatg cacaggcgtt gtaaggccat
     4981 cggctgccca cgcagaacca cgctgcgcgg cagtgcatcg atctccacga tcgcatcgac
     5041 gcctgagcgc tcaaggatgt gagccagatc tcccagcaga ccgtcggaca cgtcgatggc
     5101 actgctcgcc aggccacgca atgccagccc cagggcgacg cggggctgcg gcagctccat
     5161 ggcatcgcga acagcgtcga actcgcgccc gtccagcgac agcgtgccgc gaaacacctc
     5221 gagcgccaga cgtgcgtctc cgagcgagcc gctgacatag aggtcgtccc cggagcgcgc
     5281 gccggcgcgc agcagggcgc cggttccggt cgccgccgcc ggaacctggc cgaaaacact
     5341 gatgcagata ttcaacggac cctgcgtggt atcaccgccg atcaactcgc agcggtgcgc
     5401 gtcggccagc gccagcagtc cacgagaaaa tccgtcgagg aaagcctcgt cggaacgcgg
     5461 aagcgccagc gccagcgtga aggcgagcgg ttcggcgccg caggccgcga ggtcgctcaa
     5521 gttgacagcc agcgccttgt gacccaggcg ctccggcgcc accgtcgaca ggaaatggcg
     5581 gccttcgacc agcatgtcgc acgaggttgc gatctgcatg ccgggcgccg gcgcgaacag
     5641 cgcgcaatcg tcgccaatgc cgagcgtggc ccgtcgcgcc ggccggtcga agtagcgggt
     5701 gatcaggtcg aattcgccga gagacatcac cacattctcg ctgcggcagg tcatcgaccc
     5761 gcccggacct gcacaccgag cggcttctac aatgaacatg atgattcgcc gactccggcc
     5821 cctgatggcc ggcacgctgc cagattcgcg ggcggctgca cccagcagcg ccgccgcgct
     5881 gcgcccagac gcgccccgac gcgggacttg ctggtgcgac gaggccgcga gcccaacgcg
     5941 ccatcctgca tgcgaatccg tagtgcccag cacgaaggta tcccgatgac cacctcgaac
     6001 gacagcaaaa aagccgaact gcgccgcgcc gcactcgaat accacgagtt tccgaccccg
     6061 ggaaagatcg ccatcgcgcc gaccaagcaa ctggtcaacc agcgtgatct cgccctcgct
     6121 tactcgcccg gcgtggccgc cgcctgcgag gaaatcgtgg cggatccggc caacgccttc
     6181 aagtacaccg cgcgcggcaa tctggtggcc gtgatcacca acggcacggc tgtgctggga
     6241 ctcggcgaca tcggtccgct ggctgccaag ccggtgatgg aaggcaaggc ggtgcttttc
     6301 aagaagttcg ccggcatcga tgtgttcgat atcgagctgg ccgaacgcga tctcgacaag
     6361 ctgatcgaca ccatcgcggc gctggagccg accttcggcg gcatcaacct cgaggacatc
     6421 aaggcgccgg attgcttcta cgtcgagcgg gccctgcgca agaggatgaa gatcccggtg
     6481 ttccatgacg atcagcacgg cacggccatc gtcgtcggtg ccgccttgct gaacgcgctg
     6541 aaagtcaccg gcaaggacat caagaaggtc aagctggtca cctcgggtgc cggcgctgcc
     6601 gccctcgcct gcctgagcct gctggtgaag ctgggcctgc cgcgcgagaa catctgggtc
     6661 accgatctcg ccggcgtggt ctacgaaggc cgcaccgaat tgatggatgc ggacaagatc
     6721 gtcttcgcgc agaagacagc gcaccgcagc ctcgccgacg tgatgcgcgg agcggacgtg
     6781 ttccttggat tgagcgcggg tggcgtgctc aagaaggaag tcgtcgcgac gatgaatccc
     6841 cagccggtca tctttgcgct ggccaacccg acgccggaaa tcctgcccga ggagatcaag
     6901 gccgtgcgcg acgacgcgat catcgcgacc ggccgcaccg actacccgaa tcaggtcaac
     6961 aacgtcctgt gcttcccgta catcttccgc ggtgcactcg actcaggcgc gacgacgatc
     7021 actgtcgaga tggagattgc tgccgtgcat gcgatcgccg atctggcaca ggccgagcag
     7081 agcgaggtgg tggccgcggc gtacgcaggc gccaacctca gcttcgggcc cgaatacctg
     7141 atccccaagc cgttcgatcc gcgcctgatg atcaggatcg ctgcagccgt cgccgaagcc
     7201 gctgcggcgt ccggcgttgc cgcacggccg gtgctcgaca tggacgccta ccgcgagcgc
     7261 ctccagagtt tcgtctacgc atccggcacg acgatgaagc cgatcttcaa cctggccaag
     7321 cgcgcgctgc agaagcgcgt cgcgtacgcg gaaggtgaag acgagcgggt gcttcgagcc
     7381 gcacagatcg tgatcgacga gggtctcgcg cgaccgacct tgatcggccg tccggaagtc
     7441 atcggggctc ggatcgagaa gttcggcctg cgcctggaag cggggcgcga ctatgacgtg
     7501 gtgaacaccg acaacgacca ccgttaccgc gactactggc agacctacca tcagctcacg
     7561 gctcgcaagg gcgtcacggc acagttcgcc aagatcgaga tgcggcggcg cctgacgctg
     7621 atcggcgcga tgctgctgta caagggcgac gtcgacggca tgctgtgcgg cacctggggc
     7681 aactccgcac tgcatctgca gtacatcgaa caggtcatcg gactgcgaga aggcgcccgg
     7741 actttcgcct gcatgaacgg tctgttgctg cccggccggc aggtgatgct ggttgacacc
     7801 cacatcaact acgacccgac ggccgaacaa ctggccgaga tcacggtgat ggcagccgag
     7861 gtgatggtgc gcctggggct gcgcccgaag gcagctctgc tgtcgcacag caatttcggc
     7921 tccagcaacc atccttcggc agtgaagatg cgagaaacgc tgactttgct gcgacaagac
     7981 gctccctggc tcgaggtcga tggggagatg catggcgaca cggcgctcga cgccgactac
     8041 cgccgcgggc tgatgcccat gagcacgctg agcggggaag ccaacctgct ggtgctgccg
     8101 aacatcgatg ccgcgaacat ttcctacaac ctgttgaaaa cagcggccgg cggcggcatc
     8161 gccatcggac cggtactgtt gggcgcagcc aagccggtgc acatcctgac ggcgtctgcg
     8221 acggtgcgcc gcatcgtcaa catgacagca ctgaccgtgg cagaagcgaa cgcgacccgt
     8281 tgaagtggcc tcgtgaaggc ctcgcgtcag ccactttgtg acgattggaa gtcggtttca
     8341 gagcccgtaa atcactggca cttcagcggc tcacggcata cccaccaatc accaaaattt
     8401 atgtgagcac acacttattc acgggcggtc atgccctgaa ttgaggcgca gccttgaggt
     8461 tttcggcccg attcggtacc atcggccttt ccgaatcaag ggacaacccg tgtttttctc
     8521 tggcccattg ggggatcggc aacgcatcgc caggtcagca aaaacagccg ccatcgcggt
     8581 ttcactggct gccgcgcgat gggatccagg gatggtcagg tcccggacct cgcatcagga
     8641 aagtatcgct gaacgcacca atttggcgtt tttgcagctt cctgccgatg cccgccagag
     8701 cgatcggttg tttattccgg gtggatcagt cacgcacaag cggggcggaa ccgtgttctc
     8761 caatcggggt cgatctctgc cgggacatgc ccgcgggtat atcgcccgcg aaccaagcgc
     8821 gcatgtgcgg ctgacggccc ctgaagcccg tttttacagc ggcgacccct gcgcgagcct
     8881 cggccgcttc gcgcagtgaa cgccgctctg gttttttata cataggagga acgagtccat
     8941 gctcttgcag accgtccgtt cgaacattgt tcaatcgatc cgcgcctacc gcgtggagga
     9001 tctgatgctg gcggcgcaaa acgccgatca gcacttcctc tatgccaatc tgacgtcggc
     9061 ccaggcaaag caggacgtgc tggataccat cgccgagtca ttcttgtttc cggtgcattt
     9121 cggcaagaac ctcgatgcgc tctacgactg catgaccgac ctggttcaca aggcgggctc
     9181 tcagcccggg tttgtcgtgg tgctggaaca gctgcccgac aacccacgct tcgatcgcga
     9241 agcacgcgag cagctgctcg acgtgttccg agacgcggcc gacttctggg ccgaacgaaa
     9301 ggtcccgttc aggtgcttct attcttttca gtagcccgct ctcaagaact cggttcttgg
     9361 gagcgggcta ccgaaatacc cggcccttca gaaaagcccg tcaagcctgc agcgaagagc
     9421 gctgccggag ccagcttcgg catgaacatg ccgctgatga gcagtgcgtt cgacacctcg
     9481 atgtggttga acgccgcctg agcctgcccc cagttgagca ctggcatcaa ggcggcctgc
     9541 gggccgcctt tttcatgctt gcatcaattc aacgaaccgc ggcacacagc gacaggtatt
     9601 cggacaccgc aacctcctct gctcggcgct gcaagtcaaa gtcccccgca aagccccttt
     9661 cgtcgagcca gcggcccagc gtgttgcgca gcatcttgcg gcgctgcgag aacgccaccg
     9721 tcaccaattc gccgagcagc gtggcatcga catcttcacg cgctggcagc ggcaacatgc
     9781 gcaccacggc agatcgcacc tgagggggcg ggtcaaaaga cccgggctcg acatcgagca
     9841 ccgactcgat ggcgtagcgc cactgcagca tgaccgacaa ccggccgtag accttgctgc
     9901 cgggaacagc cgccatgcgc tcgacgacct ccttctgcag catgaaatgc tgatcgagga
     9961 cctgcgcagc caccggcaga agatggaaaa gtatcggcgt cgagatgttg tacggcaggt
    10021 tgccgaccac gcgcaggcgc tgtccggcgg cccggtggag ctcgccgaaa tcgacgttca
    10081 gcacgtcggc ctcgatcacg gtgagggccg gctgacgcct cagccgcgca gccaggtcgc
    10141 gatcgagttc gatcaccgtc agatgctcgc agcgctcaag caacgggaag gtcatcgcac
    10201 ccaagcccgg cccgatctcg atcagcgtct cgccggggtg cggcgcaatc gcatcgacga
    10261 tcgaatcgat cgcgacccgg tcggtcagga agtgctgccc gaagcgcttg cgtgcggtgt
    10321 gggccatcag agttccctcg gcggaacttg cggaatcacc gtgtccgcgc ccgaatggcg
    10381 atcactgggg tggatcgcgc aactccacat aggcgcgggc gcgcacttca cgggtccact
    10441 cggcgtaggc ttcctcgtac ttcttctcgc gcagcgcgtt acgggcttga tcccgctcct
    10501 gcttggcgtc gagcgtcgcc tgccttcttt cgtcgacctt gatcaagtgc acgccgaatc
    10561 tcgacaccac cggatcggag atgccgccga cgggaaggcg gttcattacc tcctcgaatt
    10621 ccggcacgaa ggttcccggc gaagtccagc ccagttcccc gccctggctc gcgctgccat
    10681 cctcggaatt gtctttcgcc agctgctcga aactgctgga gccatcctgg acccggcgct
    10741 tgaaatcgtt cagacgccgc accgcagcgg cctggtcaag ctgggcggtg atcttcagaa
    10801 gaatgtggcg tgatctggtc tcggtgatcg agaaggcctg accttcgcga cgctcgacca
    10861 gcttcagcac gtgaaagcct gctcctgtgc gcagaagtgc gtcggcaatc tgtccgctct
    10921 tcaaccctcg gacgcgctcg acgaatacat ctggcaatct gtcggccgta cgcagaccga
    10981 ggctgctgcc ctgcgccttg tttgcgtcct cggagatctc gagagcgacc gtcgcgaacg
    11041 actcgcccga cttcacgcgg gccatcgccg actccgcacg ggccttcctt tctgccacca
    11101 gctcctcact ggcgttctcg ggtaccgtga ccaggatctg cgcgatgttg tattcggcgc
    11161 gattggacga ttccgcgcgc tgcttggcga tcagcgcctc gatctcggtg tcggggatgc
    11221 ggatgcgggc ctggacctct cgatcacgca cgcgctcgac caggatctgg tctcgcaggt
    11281 tcttgcggaa acgggcgtaa tcgacgccgt cctccgccag cttctgtcgc agttgcgcca
    11341 gcgtcacctg attctgcgaa gcaacggtcc ccaccgcacg atcgagctcg ctgtcatcga
    11401 tgcgttgccc gctttcacgc gcgtgcgaga tctgggcacg ttcgtcgatc agcaggtcaa
    11461 gcagttggcg acgcaattca gtctcaggtg gcaaggtggt cttggtgcgc gcggcgttct
    11521 cgcgcactcg agccagtcgc tgctccactt cgcgcgcggt gaccaattcc tggttcacga
    11581 ccgcgaggat gaaatctcca cttcgcaacg aagcactcga cgccgcagcg cgaggtgcct
    11641 gggcaaatga ctggggcacc tgaccggcaa gcgcgccgac ggctacagca gctacaacga
    11701 tcgatctcaa ggcgtcagtc ataaaaggag aaggtgttct gggagctcgc cggcgcatca
    11761 cgcagcagcc ggtagccagg cacattgtcc ttcagcacct gcagcgggtt ggacccgagc
    11821 cgcgagaggc cgaccagctc cagctgtatc agcaggcgcg tggtcgcttc agcccggccg
    11881 gtcgacaggc gctccgccac gacccgtgtg atccagcaac ccgcatcgta ttcgaagccc
    11941 agaatcgaat cgacgatgcg gctgtcgcgg gtgctgtagt tgacgcggcc gacggtgtag
    12001 agcgagccct tgcaactcgc cgggtcgcca cgcgagtcgc ccgagagatt gggggcatcg
    12061 ggcgtacgcc cgaagaccgg ccattgccag ccgagttcga gctgctcgct caggtttcgt
    12121 gtctcccgga acaccgcact gacggtatgg aatggcttgg gagagtacgc agcaccgacg
    12181 atggagcgca ccgtcgtgcc cagctgcggg ctgtactgaa ctgttgccgt cagggaccag
    12241 tcgggtatca cgttcgtcga cccgagcaac agcagatcgg agatgcgctg cgtgagcttg
    12301 gttccctccg gcgtcacccg ctggtcgctg aacaggtagc gctgaaccac gccgagccgc
    12361 atcgcttccc cgccattcat cgggtcgagc agccgggtgg tcacgccggc cgtcaatgcg
    12421 tgggagtcgg aaacacgatc gacgcccgag aacccgttct cggtgaagat cgtctcgaaa
    12481 ttgaagtccc gcggcgcgga atcgaagttc ggaaggatgc tctgcttgcg gtacggcgtg
    12541 ttgacgtaaa gcagccgggg ttccagcgtc tggcgcatgt ccttgccgaa ccacgacgag
    12601 tcccgctcca gcacccaggc actgtccagg ctgaatgtcg ggatcacgcg ggaagcctgc
    12661 ttgcggccgt cggacatcgg actgtcgagc gaatacgacg cggcgttcac ggacaccttc
    12721 ggcgtcagtg tccagccggg tgtgacccac ggccggctga gactggcaat tgcgtgcgct
    12781 ctcaggccgg tcaggcggct ggcgttgttg acggtaccga tcgggttccc gaagcgattg
    12841 aattcgccct cgaagccgac ttcgaaaccc tcgcccaccg gctgcagcgt gcgcgcgccg
    12901 atctgcggca ggcgctcata gggcgcgtcg atcaggctgt cggcgttctg gagaacctgc
    12961 cagcgctgcg cccgagcgta ggtcgtccat tcgccgaacg gccgcgtcgc ctgaatgtcg
    13021 gacagcagca ggcgcggcgt ggtggtgttc aggtcgcgcc ggaaatcctt ccagtagtcg
    13081 tcatcggaga cccgctgcat ccgcacgctc agacgggtgt cgtccgccca ggctgcctcg
    13141 tgcttcgccc cgaacgcata gcgcgttcga cctgcctcgc ggtcgtgcgg caacaggttc
    13201 agattgagtt cgccgtggta gttttcctcg agatagcgaa actcgctgcc cagcccgacc
    13261 ccgcgccgaa tgctggctga cggggtgaag gtggcatccc ggttcggagc gatgttccag
    13321 taccacggta cttcgaggtg aacgccactg cggctgtcga tggcgacact cggcggcagc
    13381 cacccggact tgcgctcgtc ggtcagcggg aaactcagcg cgggcgccca aagtatgggc
    13441 acgccgagaa aacgcagcac agcgccttcg gcaaaacctt cgttgttgtt gaaatccatc
    13501 ttgacccggt cgctggtcag gagccagtcc ggatccccgc tgccgtcggc agggcagctg
    13561 ctgtaagtcg ccttggttgc gaccgtgcgt tgactgtcga tgaaatcgat gcgctcggcc
    13621 ttgccaccgg ccgcggtgcg cccgaagaag tacgtcgggt tgatgaagta gccctcgaag
    13681 cgctggacct tcagctgcaa ctcggggccg gtgtagcggt tgccttcctg gttgatctgc
    13741 acgttcccgc gcgccagggc caggtcttcg acgtggtcgt agctcagcag gtcggcgcgc
    13801 aacgttaggt cgcctctgcg cagcacggcc tcgccttcgg cgatcgtgtc gagatcggga
    13861 cgacctcgca cggtctgcgc ctgaaccgag agcggcaact gcttgccggc ttcaccgcgc
    13921 ggcagcggtg tcagggccgg cgcgatcttc agcaggacgc cgccctcggt gaccagcgac
    13981 ggcaacggcg cccgaaccgg taccggactc gccgccgggg caggggtgga gccgatcggc
    14041 gggcgtcccc actgcgccca cgccgggccg ctggccgcaa gcagggcagc cgtcagtggc
    14101 aggagctgcc acgcacggcc gcaccccggt gcgacacggg atgtcgggcc gccggtacac
    14161 agcaaacgga cgagtggacg aggcagcaag ggtggagaaa agcggtcggg aggaggtggc
    14221 gaaggccacg agcgcgaatg gcggtttgta aaattgatta tccatgaggg tgtggcagcg
    14281 actgctcccg gatgacccgc cttttcgccc ctgcgcgccc acacgccgcc ctgccatgac
    14341 tcctgtgcct ccctccaccg cacccgatgt tcgccccggt gtcgtctggg ccgatcccgc
    14401 ccgacgcgcc gacttccaac gctggatcac gacggtcgct cccgcgcacc gactgcacac
    14461 cgagacactg aggccggcat ccagcgatgc aagcttccgg cgttatctgc gcatcgatgg
    14521 ggaagcgggc agcttcatcg tgatggacgc accgccagct cacgaggatg tgcgaccctt
    14581 cgtggcggtg gcttcgttgg cacgcaaggc gcagctgaat gtgccgcagg tgctcgcgca
    14641 ggacgtcgag cacggcttcc tgctgctcga cgacctcggc acgcagctct acctgggcga
    14701 actgcaggcc gcggtcgaag ccgctgacaa cgccagcgtg gagcgcctga tgcgttcggc
    14761 gatgtcggcc ctggtcgtct ggcaacagcg cgtcgatgcc agtgccctgc ctgcctatga
    14821 cgacgcactg cttcgacgcg aactggcgct gttccccgag tggtgcgtga agcgcgagtt
    14881 cggcgtgagc tggaatgccg atgaacaggc cacctgggcg accacctgcg acctgctggt
    14941 gcgcagcgcg ctggaccagc cgaccgtcgc cgtgcaccgc gactacatgc cgcgcaacct
    15001 gatggtgtgc gatggcaaca ccgtcgccga cggcgttctg gccaacccgg gcatcctcga
    15061 cttccaggat gcggtgcgcg gtccgatcag ctacgacgtg gcatcgatgc ttcgcgacgc
    15121 cttcatcagc tgggacgagg aacaggagct cgactgggcg atccgctact ggcagcaggc
    15181 gcgcaaggtc ggcctgccgg tgcctgccga cttcggtgaa ttctggaaac cgctcgaatg
    15241 gatgggcttg cagcggcatc tgaaagtgct ggggatcttc tgccgcctca agcaccgcga
    15301 cggcaaaccg gcctacagcg cagacctgcc ccgcttcttc cgatatgcgc acaaggtggc
    15361 gacacgctac gagggcctgg gcccgttggc acggctgctg gaaccgctca tgggagcgac
    15421 ccgagtcgac gccttctact gaccttgcgg ggtccgcgag tcaggcccgc accacggtga
    15481 ccgtgcagtc cgcctgcgcc accacctgcg agctgacact tcccaggtag cgtcgcagcg
    15541 tcgagttgcc acgcgcaccc atcacgatct gatcgacctg gtttcgcgcg gcgaattcga
    15601 cgatggccgt ggccgcgtcc ggtgcctcga gcacatgaaa ggtcaggcgc ccgtcctcca
    15661 ggttcagctg cttgctgatc ggccgggccc aatgcttcag gccgaccagt tgcttgacgt
    15721 gcacgctttg tccggccgca tccaccagtt catccatgcc gatgcgtgcg gtcttcatca
    15781 cgctcacgca ggccagccgt gccccgggct cggtcacgac aatgcgtcgc acggtctcgc
    15841 gcagccgatc gagcaactcg ccggaggcct gatcgacgtc gatcgcagcc atcacgatgg
    15901 gacttctcat gacctgactg gtcacgcctt ccagcgggga ggtctgcggt tcggcgccga
    15961 tagcgaagaa ccagcgcttc aggcgctgga acgcattgct gacatgcgcg cgctgggcgc
    16021 gcgctgtgag cggtacctgt gtcgggtcct gcagcgccag cgccagctgc gcgccgctct
    16081 gccagcggcg ctcggccttc acttccaagc atttcaggat cacctcctgc agccacggcg
    16141 ggcaatccgc gcgcagcgcg cgaggcggga tcgggtccac gtagagtcgg cggcgcaggc
    16201 ctcgcaccgt gaccggtgag ccgaacggcc gctggccggt ggtgaagtgg tagagcatca
    16261 ctcccagcgc gaacaggtcg gagcgcggat cgtcgcgcac gtattgcacc tgttcgggcg
    16321 agatgtacgg cccggtgccc atcggcagcg agaactcctc gtcgagcagg tcgggaaggc
    16381 ggtcgtgatg cgacaggccg aagtcgacca tcactgcagt gccatcggcc cggaacacga
    16441 tgttgctcgg cttgacgtcg aggtgcacca ggtgctgccg atggagttcg tgcaatgccg
    16501 tggccacccg cgaaccgatc tcgatgactt cgtcgatcgc gagcggcgcc tcgtcgagcc
    16561 gcgccctcag tgacggcccg tcaatgcgct ccatcacgat gtagggctcg cggctgaaat
    16621 ccccccgcgc gacgaaacgc ggcacgtgcg ggccctgcaa tgcaggcagg atcatctgct
    16681 cgacctcgaa tccgacgatc gaggcggggt cttcgccgcc catgatgcgc ggcaccttca
    16741 tgatcagcgg cagatcggca ggaacgtcgt tcgacgcggg ctcggtcccc gagccaccgg
    16801 atacgcgggt cacacgccac aggtgggcca tgccaccggt gtgcaacttc tcctcgagct
    16861 gccagccatc gacgatggtt ccggcagcca acggcgcgcg gttggcgggc tccaacgtgg
    16921 agtctgtttc agtttccatc ggcaagacgg gcagcgagcg actgcggcaa accggccgcg
    16981 cggatcttgg ccatcgcggc ctcgtggtcg tacggaacgc gccagtaggt gagctcggcc
    17041 gcatcctcgt cgtagagcgc atagcaagcc gctggattgc cgtcgcgcgg ctgtcctgcc
    17101 gacccgggga tcaccagcca gcggcggctg gcgagcaacg ggatcgcctc acccgccacc
    17161 ggaacgaagg agcccgattt acccgttgcc gacaggtggt agagcatcgg ctcatgcatg
    17221 tggccacaga aggtgatccg ctgacgcgtg gccagcaggc tgcgtacggc ctcggacttg
    17281 ccttccacat agccccattc gggcggagcg taggcgttcg cgtgaacgaa aagcaggtcg
    17341 ccgcgttcgt gtgtcatcgg caagcggtcg aggaaatcga gctggccggc gtcgagccgc
    17401 tctcgcgtcc agtcgatcac gaaacgggct tcggcgcgca tcgtgcgccg cgctccctgg
    17461 gcgacggccg catcgtggtt gcccatgacg gccgtggcgc caccctccac atgctggcgc
    17521 acgcaatcca ccacccaggc gggatcgcca ccgtagccga cgaaatcgcc gacgaaggca
    17581 tggtgctgcg caccctgcgc ggcggcgtgc tcgagcaccg cctgaaccgc ctcacggttg
    17641 gcgtggagat cggtgatcac ggcgatcttc atggctcagg tattctccac cgcacttgaa
    17701 tcggctcggc gcagaagctt ccggatgaac ttgcccgcct cgacactcac gctgttgctc
    17761 actgtcgcgt ttgcagtggc cctgatggcg tgcgagcgta ctcgaccgaa tgtcaattac
    17821 ccgtccgact ggcgctgggt gggtcgcgcc gcgggcttcg gtatcgcggg tgtcttgttg
    17881 acgatgctgc tgggagcatg gatcgagtcc catgcctccg tgttttcctt ccttcgtcgg
    17941 gaaaactcat tgacccgcct gcccgattgg attcaggggt tcgccggcta tctctgcgtg
    18001 actttcatcg tgtactggtg gcaccgtttt cgccatcaga gcgaccggtt gtggacgctc
    18061 ttccatcaga ttcatcacag cacctatcga ctcgaggtcg ctaccgcctt ctacgcacat
    18121 ccgaccgatt tcctggcgac caccgtgatc gtcagcgcgg tcgcctacgg cttgctgggc
    18181 tatgggctgg acgcggcggc gtggacagcg ttctgggtcg gcatattcga gctgtgggaa
    18241 cacacgaaca tccggacccc ccgctggctg ggctacatca tcgttcgacc cgaaatgcat
    18301 cgcgtgcatc acgagaagga tcagcaccgg aacaattacg gtcttccggt ctgggacatg
    18361 ctgttcggga cctatgaaaa ctccatgcgg tccgtggtct gcggcttcac cagggaacag
    18421 gaagaacagg tgttgccgat gctgctcttc aagcacgccg atcgacaatt aggcccttga
    18481 cgcaatcagg tcggcggcaa acgctgggaa tgcggctcga acccgcactc cagcgcaacg
    18541 ttggcgaatt ccctggcaca gaccagctca gagcccggaa tcaacgctgt cgaagtacaa
    18601 ggtagcagac cgcttacagg ccggagggcg tgtcgagaaa tttgacagtt cctggccgcc
    18661 gctgaggtcg cagcaatgca actgcttgat ttgcctagtt ttgtgcaatt tggcgcgaaa
    18721 tatgcttttg ccgcggaaag tcagccccgc tggtttgatc ggatcggata gcacatgcag
    18781 ttctacgcaa gagcactcgc aaccgccctt tgcctgtccg cagcagccac gggaattgcc
    18841 caggcagccg tgatcacaca gacgggcaat ttcagcctgg catcggcgga gttgaagcgc
    18901 cgcgatagtg gaaacagctt taacagcacc gtagtctcag ttttcagtga cccgcagtcg
    18961 ctcagccaga gcatgagctt tgatcttttc gattcttcgc tcggcatcct gacaggcgtt
    19021 acgttctggc tctcgaatcc gttaggcacg gacattctga cgctcagtga aggtatcgcg
    19081 caggacaacg gcctcaccca ggctgccggc tccgtgactc gtacgctgac cgggccctca
    19141 ggcccgagca tctcgttggt cagtgccttc ttgggtaccg cttgtggctt ttccgacacc
    19201 tgcatgacgg atacgctctc ccacagcccg ctggcgacag acgttggcgg cgaaagccta
    19261 gtccccctgg ccgggttcat cggccaaggc ggcttcgaca tcactgcagg cttggcgctg
    19321 gccgcaccaa cgatctcgta catcggcggc atccccgatt gcgacagcac ggaagcaacc
    19381 ttgacgagtt cctgggccgg cacggtcagg ctcgaatacg cctacaacgc cgcaccacca
    19441 tccaacgaag tgccggagcc cggaagcctg tatctggcag gcgccgctct gctggcggcc
    19501 atcggcttgc gtcgccgtct ggcctgaaca gacaacacgg gcaggcactt cccggcaagt
    19561 gggactcagg gcgcaaccgg cagcactcgg taggcgctta gccccagcaa tcggttgtaa
    19621 atcggctcaa aaaatctggc gtcgtaccgc cccagcagat acatctggtt caggttggag
    19681 gcataggccg tctcgtccag caggtagacg gccttcactt ggccgcggtc ggtcagcaat
    19741 tgcaccgtca gccccggacc ctggttcagc aaatgctcct ggctgacctg cccgtcagcc
    19801 aacaacacga cccggcgcag cggccggggc gcggatgccc ctccggcggg cagcccggtg
    19861 cggtgcacca gcgccgcacg caagtcgacc gtcacgccgt cgcagcgcag taactctgcc
    19921 gacaagctgt tgcaggccag ttgcacgatg ccaaatgggc gatggcgtcc tttcgcgact
    19981 gtcatcggcg tggccaagct ttcgatgctg ctgaacttgg gaagcatgtc cggcgtgaag
    20041 accaccagca ccggcacctc catctgctca ggagccgcct gacgcaggcg gtccagcaag
    20101 ccgtcgaggt cctgcgcatg cgctgcctgc gccacgatgc cgctgttgcc tgcgtggtcc
    20161 acgaacctca ccagggcatg cagcagcgcc gggtcgggcc gggcaaggct gaccgccacc
    20221 atattggtct gcggcgtgaa ctgcaccgca ccgtcgtggt agacgccaag gccggtggta
    20281 tccacgatgg caaagcccaa gtcccaccag gtccacagtc gggtgtcggc tggcacctgg
    20341 gtggccaagg ccttcaggtc ttggtagaca ggagcaggaa tcgcaggcgg cggcgcctgc
    20401 agacgatcgg cagcagggcg gaaccagacc acgaacacca gcaccgcagc cagcgaggcg
    20461 accaccgcct gcgcttcccg gccggcaagc tttgcccgta gaacagccag cggtgcgccg
    20521 cctgcagacg caggacgcgg cggttccatc gggcgccccc gcgcctcagc cggccgcagt
    20581 gtcagaccga ccagccatgc gatggcgatg ccccagcctg cgcccacgaa gggcgcaaga
    20641 tacaagacga aacgccgtga gccgaacatc gccatcgcac ccagcagcac ccaaggcagc
    20701 agcggcagaa gggcccgcca acgcggcagg gcccaggcca aaaagaacac cccgccgagt
    20761 gccgcccacc cggcgtgcgg cagaacgcgc tccagggtat cgcccacatt caaggactgc
    20821 acctcgctga tggtcgccca gaccgtggtg aaacgcagcg caggcggtaa ctgcaagacg
    20881 gcccgatcgg gccacaggta atgcgccccg aagtccgaga ggctggccag cgcgtagacg
    20941 aactgctgcg gccccgagca caacacgaaa agggctaccg ccggcagcca gcgccgccaa
    21001 ggtgtgcctg ccaatacgag gtgcagcgcc agcaccgctg ccagccccag cgccagcccg
    21061 ggcttccagt accactgcga atagacaagg ttcaccagcc cggccaccgc cgcccagccc
    21121 cacagccgcc aaacctgacg gtcgctgccc atctgcagga acagcgccac cagcagccag
    21181 ggaaacagca ggttcaactg gtcggtgtcg gaccagccta ccccggtacg ggccagatac
    21241 tccaggcaga aggtgccgac cagccccgcc atcacgccgg ccgccggcac gcccaggcgc
    21301 caaaacaccc agaacagggg caccatgaac aggctggaca gcaatggcgg cagcatccag
    21361 gccgtacggt cgatgcccat gccggaccag gctgccacgg acgcgatcaa ccggctaagc
    21421 aacggcaggc ttcgcggctc ccactgcgcc gcccaggcat cgggctggcc tttcggaggt
    21481 cgggtgaagt gccgcggggt ggccggtgcg cccggctcga actggccagc cgcccactgg
    21541 cgagccaaac gcagggcgta ataggcatcc accgtggaca tcattgggat gccatcggac
    21601 tggtagtgcg cggcgtcgcg ctcccacgca gacttttgct gccaccgcac gtggctggcc
    21661 gcgaccagcg ccaagatcac cagcaccaga cccaggacca agcttgcgcg gcccgctggc
    21721 tgaaggacga atgaacgtgg aacccggggt aacagctggc gcataggcga tcgggaagct
    21781 gacggattat ctggcctcca tcggtggcgc gccttccggc agacacgcgc ctgccccccc
    21841 ctacccgcgg gtatcagcca gccgcacgtc atcggcggct tgacccgccg cgcagcagca
    21901 tgagaacatc gattttcaat agtcaatcgg acggttgcca ccatgaccaa gccctctgcc
    21961 gtgatagcac aagcccaaga agcgctggac acttccattg agcggcggcg agccatcctc
    22021 aaacgtctgg gccagacttc ggtcgttgct gcggcggtgg ccgcaccggt ggccgcctcc
    22081 gcgggtgtgc tttcccgctg gaacctcgac ccgtccaagc gcacgggtag cagcggcaat
    22141 ctgaagctgg gcgtttccag ctgaactgcc gcgggcttgc cggggtcgac gcggtgcgga
    22201 tcgaccgagc cgtcgctgtg gcccatgcgc cggggagcac gcaggctcca caccctcgcg
    22261 cccgacgcgc agtgcgcgcg acatgctgtt ttcgttgaat ccccagatcg acctgatttg
    22321 gcgcgattgg ggcgacgaat ctgtggcgat ggaagcggcc tcgggccgcg ttttcttgtt
    22381 tgaccctctg tcctcggcgc tgctggcctg tttcgagtcc ggcccgcaac aggtgcaggc
    22441 tgcggtggat agcctgttgg ctgacttggg ctcgccgacg gacagtcgcc ttcaggagtt
    22501 cttgagagct gccttagatc ggttcgtagc cctgggttgg ttgctgccgg gcgacgcctg
    22561 agtgaagctg ggccaactcg gattgcaggc cctgggcaag cgcctgcgcg gccccggctt
    22621 ggcatggcgc agtggtccgt tctccatccg cctgcgcacc gaccttgtgg ccgtgcgcga
    22681 cggcctccac ttcctgtacg ccgaccatcc cctggtgccg ggcggcgact tctgtgacgg
    22741 cgatatcacg ctgcggcagc cacgcgcagc atggggatgg cggcggccga tggtgcaggc
    22801 gcatgtcgac taccagccca ttttcgaacc tgtgccggta gcccacgcgc tgccgctggt
    22861 cgagtggctg ctgaattggc acgtcaccgc gcacggccac cacttcctga ctgtgcacgc
    22921 agcagcgctg gaacgcgatg gcaaggcctt gctgctgccc gccccgtcag ggtcgggcaa
    22981 gagcacgctc tgcgcggcgc tggtgcatcg cggctggcgc ctgctgagtg acgagtacgc
    23041 gctgatcgac ttcgcgaccg gactggtgca cccgctggcc cggccagtga gcttgaagaa
    23101 ccagtccatc gaggtgctcc gcgcctttgc gccggaggcc gtgttctcgg ccccggcgca
    23161 cggcacgccc aagggcacgc tcgcgcagat gaaggcgcca gcggctcacg tgcaacgcat
    23221 ggacgagccg gcttgtccgg tgtggatcgt cttcccgcag tggtcgcccg gcgcgacgtt
    23281 gctgctgcaa gcgcggccca aggcccgcgc cctgctggag ttgactcgcc agaccgtcaa
    23341 cctggacctg gcaggacatg atggcttcgc acgtctggca gaactggtca cggccagcga
    23401 gtgcctggac ctgcgctacg acatgctggc cgatgcggtg gccgccatcg acgcaatggc
    23461 ccaggcgatc gaccatgccg ggtgacttgc tgaccgggct gtggaccagc aacaccgcgc
    23521 cacagtggca cgcggccgat tgggaacgtc tattcagcca ggcccggcgc gcccgtcttc
    23581 tcggccgtgt ggcctggctg ctgcaaacgc gcggctggac cgattgcgca ccgcccgagg
    23641 ctcgggtgca cctgcacaac gcgctgcgtg ctgtcgcacg ccagcggcag gcagcccact
    23701 gggaggttga ttgcgtccga cgcgcgatcg gccacctgcc cacgccggtc gtgctgctca
    23761 agggtgcggc ctacgtgctg gccgatctgc cgccggcgaa gagccgacgc ttcggcgaca
    23821 tcgacatact ggtggcggcg cctgcgctcg gggccgtgga ggccgcactg gcggcgcatg
    23881 gatgggctgc catgccactg gacccttacg acgaccggta ttaccggcag tggagccatg
    23941 aactgccgcc gttgcggcat gaggagcggg gcacgatgct ggacgtgcac cactgcatta
    24001 cccaacccgg ttcgcgcttc gccgtggatg cgcaacggct gctggccgac gcccggccgc
    24061 tgcagggcca acctggcctg atggtgcttt cgccagccga catggtgctg cacgccgccg
    24121 cccacttgat gcaggagggc gacctgtccc gcggcctgcg tgatctgctc gacatgcatg
    24181 acctgctgac ctgcttcggc gcccagcccg acttctggcc ggcgctggtg gaccgggcag
    24241 ccacgctcgg cctgtgcgag cccttgctgg atgtgctgac ccaggcgcag tgcaagctgg
    24301 ggctggtggt gccacaaccg gcatggcagc agctgcagca acaatcggcc cgcccgctgc
    24361 gccaccggct gacagcggcg atgctggccg tcgcgctgcg tccgaaccac ccggacgccg
    24421 actgccgcag cacccgcctg gtgcgagcct ggttctatct gcgggcgcac cgaatccgca
    24481 tgccgctgca ccgcacgctg ccgcacctgc tgcacaaggc ctggctgcgt ctgcgccatc
    24541 gcccacccgg actgacgtga gcattcggcg cttccacagt aagtgaccag ccaatcttgg
    24601 ccagcgatgc agcaactgcg cgtcggtttc gctggctcga tgcgcgggca agcgctcaag
    24661 tacgtccttg aggtggacgg tgcgcccggt gcctggatca gctccgagat cgtcagctag
    24721 cttatggatt cccttccaat cccgtgagac cgctttgaaa gcgacgctat ttccctatac
    24781 agaaccggct gaatatctcg cctagcaatt cgtcaggcgt tgattgaccg acgatcgcac
    24841 cgagggcgtg ttgcgccagg cgcagttcct cggccagcag atcgagagcg ggcgccacac
    24901 cggaccattg gcgagtcgct tcatcgacat gcgcacgcgt ctgctgcagg gcttgaacgt
    24961 gtctggcgcg ggcggcgtag acgccctcgg gatggtcgag ccagccggcc agttcgagca
    25021 gacgatggcg cagcgtgtcc agccccaagc ccgtcgtggc tgacagacgc aattccgacg
    25081 gccctgcgga gcacccgtgc ggcaaatcgg ccttgttgta gacctcgagc acccgtccgg
    25141 cttcgaccag cgcacgcggc agccgctggc ggatggcgga ctctgcggcg tcgtggtctg
    25201 gttgcccgag ccgcgtgaga tcgtgcagga agacgagcgc gtcggcctgc tcgatctcgg
    25261 tccagctgcg cgcgatgccc atctgctcga tggtgtcgtc ggtctcgcgc aggccggcgg
    25321 tgtcgatcac gtgcacgggc acaccttcga tctggatcgt ctgggtgacc ttgtcacgcg
    25381 tggtcccggg gatcggtgtc acgatggcga gttctgcacc ggcgagtgca ttcagcagcg
    25441 aactcttgcc cacgttgggc tgaccggcga taaccaccgt gatgccgtcg cgcaacaagg
    25501 cgccctggcg tgtccgcgcc tcgatgccat gcagtttttc cgagatcgac gaaagctgac
    25561 cctgcgcatc ggcctgctgc aggaagtcga tctcttcctc gggaaaatcc agagtcgcct
    25621 cgaccagcat gcgcaggcgc atcaaccggg catcgagctc gcggatctcg gccgagaagc
    25681 caccgctcag cgagcgggag gccgagcggg ccgctgcctc ggtgctggca tcgatgagat
    25741 cgctgaccgc ttcggcctgg gccaggtcga gcttgtcgtt gaggtaggca cgctcggtga
    25801 actcgcccgg catcgccagg cgcagacctg gcagcaccgg caggccggag gaagggtcga
    25861 gggtcgaggc agcctcaatg cagcgcgcca gcagcagttg cagcaccacg ggaccaccgt
    25921 gcgcctgcaa ctccagcacg tcttcgccgg tgtaggaatg cggcgcgggg aaatggatgg
    25981 ccaggccgtg atcgatcggc ccgccattcg catcgagaaa cggcagcagg gtggcgtgcc
    26041 ggggcgccag cgacctgcgg cacaacaccg cgatcagtgg ccgcagatca cggccggaga
    26101 ctcgaatgat gccgacggca gcgcgccccg acgcggtggc gatggcaacg atcggatcgt
    26161 gatggcgcgg cagcatgcag cgcattctct tcggtggaga ccccgccgca cgccgtcacg
    26221 ccatgcggcg cagcgctcta cttgccgagc actccgagct gcttgttgat catgtactgc
    26281 tgcgcgatcg acaggatgtt gttggtcaac cagtacagca ccaggcctgc cggaaacacg
    26341 aagaacatga tcgagaacac cagcggcatg atccacatca tcttggcttg catcggatcg
    26401 gccggcgtcg ggttcaacca ggtctgcaac aggctggtga cagtcatcag gatcggcagg
    26461 atgaaatagg ggtccttggc cgacaggtcg gtgatccagc cgatccacgg cgcattgcgc
    26521 atctcgacgc tggccagcag cacccagtaa agcgcgatga agaacggcat ctgcacgaag
    26581 atcggcaggc aaccgccgag cgggttgacc ttctcctcgc ggtagatgcg catcatctcc
    26641 tgctgcatct gctgcggctt gtccttcagt cgctcgcgca tttccatgac gcgcgggttg
    26701 acggccttca tcttggccat cgatcggtat gcggtcgcat tgagccagta gaaggcgatc
    26761 ttcagcagga acaccagccc gacgatggcc catccccagt tgccgatcag cttgtgcagc
    26821 tgggtcagca gccagaacaa cggcttggag agcacggtga gccacccgta gtccttgacc
    26881 agctcaagtc caggggcgag ctggctcagc ttgtcttcct cctgcggacc ggcgaacagg
    26941 cgcgcgtcga gcgtctgcgt cgccccgggt gccaacgtgc cgagcggcac gatcatgccg
    27001 accgagtact ggttcgtgtc gaccttgcga gtgaagaact cgcgaggccg cttctcatct
    27061 gccgtggccc ccagcagcca ggcaccggcg aagtagtgct gcaccatcgc gacccacccg
    27121 ttgtcagcgg cagtgtcgtg gtcgggcttg tcggttccgg tgcgcttctc gatgtccttg
    27181 aactcgacct tgcggaactt ggctgcgtcg gtgtagaccg ccggacctgt gaaggtgaaa
    27241 tagaagctcg actcgccctc cggcgcattg ccgtcccgca cgaactggta atacacctgg
    27301 ggcgttaccg gcgtggtcga cacattgacc acatcatgct tgacggccac cacgtagctg
    27361 ccacgctgga atacataggt cttgatcagc ttgatgccgc ccacgtcggg cgattcgagc
    27421 ctgacctcga gccgatcctg atcctgcttc agcgtgaggt cgccggggat caggttgaac
    27481 acggtcaggt ggttgggcat cgctgctcct ccctgagcgg caatcagtcc ggtctgcgcc
    27541 ttgtagaggc ggctagaaga ctcgtcgaac aacacgacat tgcggtcgcg atccgcgtgg
    27601 tcggcctgct tcaacagttc gagtcgaacc atcgagccgc ccagcgtatc gaccgtggcc
    27661 ttgaccagat cggtggtgat gtcgaccttc tgcgactgca gaggtgcagc cgtcgacgca
    27721 tcggcgacgg gagcagctgg catggcactg ggggtgcccg ccggcagcgc cgcagcggcg
    27781 ggtacggcgg cagaaccggc aggcatcggg ccgctggcag cgggctgtgt cacctgaggg
    27841 gtgccgaaca gggacggctg tccggtgtgc ttgttccacg cgtcccacaa caggacaagc
    27901 gacatcgaga acaccaccca caacagggtg cgacgcattt cggtcatgag aacaacttct
    27961 tgttagggac gggatcgtga ccgccgggac accagggatg gcagcgcagc acgcgcttcg
    28021 cggcgagata gcttccgacc gctgcgccgt gggcttcgag ggctccgatg gcatagacgg
    28081 aacaggttgg ctcgaatctg caggcagagc cgagccaggg actcagcagc aaacgataca
    28141 agcgcaccgt tcccaccagc gcgcgctgcg ccacagacgg ggccacccga ttcatcgcgt
    28201 cgccctcgcc acggcagcgc gcatcagcgc agccagttcg tccgttacga cggcgctgag
    28261 gcgatcggac gccgcgctga cgaactcggt gcgagcaaaa ggcgtacgca accggacgac
    28321 ccacatgcct gctggcagcc cgcagacatt gctgacggca gaccggattc ggcgcttcag
    28381 caaggttcgc gtgaccgcgc gcttggcatg ccgcttcggc accaccaacc ccagccagca
    28441 acctgtcagg gcagactcat ccacaggcgg acaggatgta ggtgcaggtg ctgttgacaa
    28501 ctctacgcga cctgtgcccg gagcgcgcgg cagatggtgc accgcgaagt gcggcgaacg
    28561 gacctgacac ggcgcgttca gcacctgggt gaagtcttgc gcctgcgtga tgacgtctat
    28621 cacagcggtc gcgcgggaga agcccctgtg atgtgcggca tcacgagggg catcggcatc
    28681 agaccgccaa acgcttgcgg cccttggcgc gacgagcgtt gatgatggct cggccgccac
    28741 gggtcttcat gcggaccagg aaaccgtggg tgcgggcgcg acgtgtcttc gaggcttgat
    28801 aggtgcgttt catgacgaat ccaatacgga atgaaggcgc cttctcgcga ggcgctcagg
    28861 gatctgacgg gtgttccgac agtcagccga cactcgttcc aggctcgatc gcagggttgc
    28921 gactcggctg gatcgatctc ggggcaacct cggattagac caagaaagcg cccgcacgtc
    28981 aaaaccccag cgttccaccg gtctggtcga ttcacctttc ggcacgtcct tttcgtcatt
    29041 tcgagctgtg gataactcga aacctcaggc acctttcgag tagactggcc gacctggaaa
    29101 gcgcggttct tcacaacatg accgaagacc tctggcaacg cggttgcgag cgactcgccg
    29161 ccgaactgcc cgaacaacag ttcaacacct ggatccgccc tttgccggct gctgtggttt
    29221 ccgaaaccgt ggacggtgcc accgcgtgtc tgctggttcc aaatcggttc aagctcgact
    29281 ggatccgcaa ccagtacggt gggcgcatcg aagccgtgtt gtccgacctc gcgggcaagc
    29341 ccgtgaaact ggagatcaat ctggcgcccc gagacgcgca gccgcgcgca gccgccctgg
    29401 tgacgccagc cacggttgca tcgcgcgccg tcgattcgag gaatgcaacg cgttcccatg
    29461 acccgatcga tccatattca gcgtcgacgg tcgaggtcaa cagctcgtcg acgccctcag
    29521 cacagggcaa cggactcaac cgtcaccggc tgaacctgag cctgacgttc tcgaacctcg
    29581 ttcccggacg cgccaaccag atggcgagaa ccgcggcgct gcatgtggcg ggtgctccgg
    29641 gccagatgta caacccgctg ttcatctacg gcggtgtcgg cctgggcaag acccacctga
    29701 tgaacgccgt gggcaacgcc ttgctggccg atcggcccga tgcccgggtg ctgtacctgc
    29761 acgccgagca gttcatcacc gatgtcgtca agaactacca gcgcaagacc ttcgacgaac
    29821 tgaaggcaaa gtaccactct ctcgatctgt tgctgatcga tgatgtccag ttcttcgctg
    29881 gcaaggagcg cacccaggaa gaattcttca acgccttcga ggcgctgctg gccaagcgcg
    29941 cgcacatcat catgaccagc gacacctacc cgaagggact ggtcgacatc gacgagcggc
    30001 tcacttcgcg tttcgatgcg gggctgacgg tggccatcga gccgcccgag cttgaaatgc
    30061 gcgtcgccat cctgatgaag aaggccgaag tcgaaggcac gacgatgccg gaggacgtgg
    30121 ccttctttgt cgcgaagaat gtacgtgcca acgtgcgcga actcgaaggc gcgctgcgca
    30181 agatcctcgc ttactcgcgc ttcagccaga aggaaatcaa catcgcgctg gcccgggacg
    30241 ccctgaagga tctgctgtcg atccagaacc ggcaggtcgg cgtcgagaac atccagaaga
    30301 ccgtcgccga cttctacaag atcaagatcg ccgacatgta cagcaagaag cgtccggcca
    30361 gcattgcgcg tccgcgccag atcgcgatgt acctggccaa ggagatgacc cagaagagcc
    30421 tgcccgagat tggcgagctg ttcggaggtc gcgaccacac gacggtgctg cacgcggtgc
    30481 gcaagatttc cgccgaacgt cagaagaaca ccgaactgaa tcagcaactg cacgtgctgg
    30541 aacagacgct caaaggctga ccgatgaaag accacgatcg aagcgcctgt caaggcacag
    30601 acctggggac aacttcaggg caatttcgac ttgtccactg ccgcgaggcg aaattcaaag
    30661 ttgttgtctg cggctcccgg acatcgggag cttctcttca cagggttcgt ctacacgcaa
    30721 ccggttgatt taaagacaga aaatacggtt ctgcacaggt tggcgtcacc cctactacta
    30781 cggctaattt gagaggttca catgattgtc ttgaagacag cgcaagaaaa acttctgggt
    30841 gcgcttcagt cggtttcggg aatcgtcgag cgcagacaca ctttgccgat cctggccaac
    30901 gtgctcctgc gcaagaacgg gggtgagatc gagttcacca cgtccgatct cgagatccag
    30961 gtgcggaccc gcagcacgtt cgatggtgac ccttctgcct ttgccacgac ggtcggtgcg
    31021 cgcaagctga tcgacatcct gcggtctctt ccagccgatc agatcgttac cctgagcgcc
    31081 aaccagagca agctcacgct gcaaggcggc aagagccgct tcacgctgca aacgatgccc
    31141 agcgacgact ttccgctggt gcaggaagct gccgatttcg ggccggtgtt caaggtgccc
    31201 cagagcacgc tcaagggcct gatcgaccag gtgcacttcg cgatggctgt gcacgacatc
    31261 cggtactacc tcaacggcat cctgttcgtc gccgagggaa agagcctgac actggtcgcc
    31321 accgacggtc accgcctcgc gctggcgcag gcgacgctgg acgctgaaat accgaagcag
    31381 gaagtcatcc tgccgcgcaa gacggtgctc gaactgcagc gtcttttgaa gagtgacaag
    31441 ggcggcgacg acaacctgat cgagatgcgc ttcgccggca accaagccaa gttcagcttc
    31501 agcggcatgg agttcgtcac caagctggtc gaaggcaagt tccccgacta caaccgcgtg
    31561 atccccaaga accacaagaa cagcgtgacc ctgggccgcg ccaccctgct ggccagcctg
    31621 cagcgcgcgg ccatcctgac gagcgagaag ttcaagggcg tgcgcgtcaa catcgagccg
    31681 ggcaccctgc gcattgccag cagcaatgcc gagcaggaag aagccaagga agagatcgag
    31741 atcgactacg gcggcgacac catcgaggtg ggcttcaacg tgacctacct gatggacgcg
    31801 ctgagcaaca tcagccagga catggtgaag atcgagctgc aggacggcaa tgccagcgcg
    31861 ctgatcaccg tgcccgagca aagtggcttc aagtacgtgg tgatgccgat gaggatctga
    31921 cgctgcgcgt cagctccgca tcggcatcga tgccattccg tggggcaccc cctgcccccc
    31981 gccaggcggg ggctccagtt tgaatgacga tggccgcagc agcggcctgg aagaccatcc
    32041 atgaccgacg ccaccctgcc caacaacccg aagaagacca ccagctcggg cgtgacgcaa
    32101 cgcgaggtgc ccaccgccga aggcgctgcg gcgtccaccg gcaacgaggc atcggcggcg
    32161 tatggcgaag gcagcatcca gatcctggaa ggcctggaag cggtgcgcaa gcgcccgggc
    32221 atgtacatcg gcgacacgtc tgacggcacc ggcctgcacc acctggtgtt cgaggtggtg
    32281 gacaacagca tcgacgaggc gctggccggg cattgcgacg acatcgtcgt caccatccat
    32341 tccgacaaca gcatcagcgt catcgacaac ggccgcggca tccccaccgg cgtgaagatg
    32401 gacgacaagc acgagcccaa gcgcagcgcg gccgagattg cgctgaccga gctgcatgcc
    32461 ggcggcaagt tcaaccagaa cagctacaag gtgagcggcg gcctgcacgg cgtgggcgtg
    32521 agctgcgtga acgcgctgag caagtggctg cgcctgacgg tgcgccgcga gggcaaggcg
    32581 cactacatgg agttccgcaa gggcttcccg caggaccgca tcctcgagca gcgggatggc
    32641 ttcgaagtct cgccgatgaa gatcaccggg gagaccgaga agcgcggcac cgaggtgcac
    32701 ttcctgcccg acgacgagat cttcgaga
//
LOCUS       DAOORV010000022        32288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1597_length_32288_cov_9.312568, whole genome shotgun sequence.
ACCESSION   DAOORV010000022 DAOORV010000000
VERSION     DAOORV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 32288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32288
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_32288_cov_9.312568"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKV56_04850"
     CDS             <1..965
                     /locus_tag="PKV56_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX67174.1"
                     /translation="RFTDAWLSMAGTADIGIPNGLPVDEWGIRVAADKCTPVGASVSR
                     GGATNSPAAVYALTKYVDWMKKYAPKDASGMTFGEAGPVPAQGQIAQQIFWYTAFTAD
                     MIKPGLPVVNADGTPKWRMAPGPNGPYWKQGMQNGYQDVGSWTFFADHDADKTAAAWL
                     YAQFITSKTVSAKKSVVGLTFIRDSDIRHDYFTKNAAKYGGLIEFYRSPARVAWTPTG
                     TNVPDYPKLAQLWWKNVAVAVTGEKTPQAAMDNLAEEMDQVMARLERAGMAHCPPKLN
                     PKGDPAKYLSDKGAPWAKLANEKPKGQTIGYDTLLSAWKAGKVR"
     gene            complement(987..1943)
                     /locus_tag="PKV56_04855"
     CDS             complement(987..1943)
                     /locus_tag="PKV56_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOX67175.1"
                     /translation="MFKNLTVYRVGPEWSATLEQIEDGLAAARFAECGATQPQSAGWV
                     APRGTEHGPLVESVGGQWLMTLMIERKALPSAVVKRRTDEMAQQVEHSTGRKPGKKQT
                     KELKEQAVLELLPMAFTKRSTLKVWIDAKQRLLMIDASSPARAGEVVTQLIKSLDGLT
                     LTQLQTTESASVAMSDWLLTGEPPASFSVDRECELKSEDEMKSVVRYARHPLDIDEVR
                     GHIASGKRPTRLALTWQGRVSFVLTETGQIKKIDFLDVVFEGRPSRDSRDDAFDADAA
                     IATGELVQLIPELIDALGGELLLVAPPAVALTGDGARAFTAA"
     gene            2102..2680
                     /locus_tag="PKV56_04860"
     CDS             2102..2680
                     /locus_tag="PKV56_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOX67176.1"
                     /translation="MESLFVSTGVVALAEIGDKTQLLAFILAARFKKPLPIILGILCA
                     TVVNHGLAGALGAWITTTISPEMLRWILGASFIGMAIWTLIPDKIEEEETQIASKLGV
                     FGATLVTFFLAEMGDKTQIATVAMAAHYPNPVLVVIGTTLGMLLADVPAVFVGDKLAS
                     KIPMKLVHSIAAAIFAALGVATLLGAGSKLGF"
     gene            2819..4003
                     /locus_tag="PKV56_04865"
     CDS             2819..4003
                     /locus_tag="PKV56_04865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX67177.1"
                     /translation="MNDKLKQAADTVLANTVGCAGGAPGVVAMVTDRKTNVYEGAAGR
                     RSLGGDAPMTTDSVMAIFSTSKAITGTTLMQLVEEGQVRLDDPVRKYVPEIAEIQVLE
                     GFDAAGQPKLRAPKRDITINHLMLHTAGFSYEFFSADDLKLRGAKGIPSVVSSTFASV
                     KTCLLWDPGEKWGYGVNIDWVGKVVEAVRGKRLGDVMQERVFAPLGMNDTSFVMTPSM
                     QSRRAVIHDRAMDGKLTPLPDLVLPQPPEMDMGGHGLYSTVGDYMKFIRMILNDGAGP
                     NGRVLKTETVDQMSRNGLGALKSGGWTTSIPSLSNTGEFFPGLPKSWGYTFMINDEAT
                     PSGRPAGSLMWAGLANLFYWIDRESGIGGFWGSQILPFQDVSSYPGFVDFETAVYRHL
                     KR"
     gene            complement(4059..5738)
                     /locus_tag="PKV56_04870"
     CDS             complement(4059..5738)
                     /locus_tag="PKV56_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67178.1"
                     /translation="MNLPRTLCLAALLSTLIGLSSCGGGSDQERSIYPTSGTYGWIVK
                     PGAGGDRLSLIHPSQPDTEYAITQSSDGPRWWLIVSSGTVNASTQQVTDVRAHTIVYD
                     AGQSVRSVSLEAHGAAPVSKPPSSGLAESCNNLGVVGNDYTNPLNSRLIVPRRSRGGI
                     NCFNESDNPDDVYEGLLELRFSASGELVSTVLSTTPADPIPVGVLRDTATLAPRAWLY
                     RDRVVLWADGTTIPLSPPGTTDTQIVLASTDRQALVQRPGGLSVLDFPSGSTLIETPL
                     NPAVTAGFGPFYRGTFIFDDDAYYLGASSFDPSTRSDVWKVLKITRSSPVASVLVSGT
                     DSISELRLGSNALYAISLTIPGMAGGGPRATHRNLIRISKTDGSVQKTAYPIGITPTI
                     HPGAAGVHMLELEDQNSFSLLPAGERRVLQFIDEDNNTLYTADQGYGVGAHEDTNTVN
                     FGRSVHAERFFFAANNAQDFYAATLTVYDTRTRQATALGPLPGTADYADTYADGVEPD
                     GILAPGPQSFGAGVAARSVVGWGFFIASNDNKVFSFDISKPNSLRYTTTVR"
     gene            complement(5803..7050)
                     /gene="glcF"
                     /locus_tag="PKV56_04875"
     CDS             complement(5803..7050)
                     /gene="glcF"
                     /locus_tag="PKV56_04875"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HOX67179.1"
                     /translation="MQTNLAPEFKGTPDGDSAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGAEPTRKTQQHLDRCLTCRNCETTCPSGVQYGNLVEIGRRIV
                     DGKVERPRGEKAVRWLLKEGLTSPLFDTALKLGQFARPLLPAALKGKVAATPGPRERA
                     LAHRWPKREHARKVLMLMGCVQPAMLPNINSATARVLDAAGIQTMVAEEAGCCGAIRS
                     HLGDQAGGLADMRRNIDAWWPSVISNKIEAIVMNASGCGATVKDYGHALAHDEAYADK
                     AKRISELTRDLSELLPDLVPLLKPRIHRKPTARLAFHPPCTLQHAQQLRGGVETHLGA
                     LGFDVRLSANESHLCCGSAGTYSVLQPTLAHQLRDRKLANLAEVEAQVIVSANVGCIQ
                     HLQTGTPTPVKHWVEVLDEALAG"
     gene            complement(7069..7800)
                     /locus_tag="PKV56_04880"
     CDS             complement(7069..7800)
                     /locus_tag="PKV56_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOX67180.1"
                     /translation="MPNFVELWVYLSATPLFGLTATLVIYVIAQGVYSRLGQAPWANP
                     VLWTVIALALLLTLSGTPYPTYFSGAQFIHFLLGPAVVALAWPLWQRRSELRERALAV
                     TVAALAGGLAAAGSAVAIGWSLDLPAELLRSLAPKSVTAPVAMGIAEQLGGIPALAAV
                     FAVLTGLVGALSAKYLFDALGITAHSVRGFAIGTAAHGIGAARALQVHPDSGAYAGLA
                     LGLQVLLASLLLPWFIPLLLRLLPA"
     gene            complement(7820..8215)
                     /locus_tag="PKV56_04885"
     CDS             complement(7820..8215)
                     /locus_tag="PKV56_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOX67181.1"
                     /translation="MTSTAAPLQGLAWLLACQSAGELLARAAPIGLPGPVIGMLILML
                     ALGWEPVRGPVGAIADVLLAHLSLLFVPVGVGVMTHMALVSQYGARLAVALLLSTWIG
                     LAVTALVLRALLPKATSTGLPKPDPAAPR"
     gene            complement(8238..9383)
                     /gene="glcE"
                     /locus_tag="PKV56_04890"
     CDS             complement(8238..9383)
                     /gene="glcE"
                     /locus_tag="PKV56_04890"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HOX67182.1"
                     /translation="MENEDESPALTRLIERVRSARDCGTPLRIRGSGTKDFYGELPQG
                     ELLDTTELSGISSYAPTELVVTARCGTPLSELEAALAEKGQCLPFEPPHFAGDTPEAT
                     AQGGTVGGMIAAGLSGPSRAAVGAVRDYVLGATLLNGRGEVLSFGGQVIKNVAGYDVS
                     RLLVGSLGTLGVILEVSIKVLPVAPATTTLRFDMEAAHALAQLHQWGCQPLPLNASAL
                     WNGMLVVRLRGAAAAVQTATSQFLALGGEVIGPDLADPFWAGLRDHQDEFFMGAQAAV
                     QSGGALWRLSVPQTAPMLDLPGTPLIEWAGGQRWCVTPDASPETAAHLREIAGRAGGH
                     ATLLRSLDKAAGVFAPLSSPLDRIHRELKRSFDPHGLFNPGRLYPGL"
     gene            complement(9398..10921)
                     /locus_tag="PKV56_04895"
     CDS             complement(9398..10921)
                     /locus_tag="PKV56_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOX67183.1"
                     /translation="MNAPSTAAELSPAPQPDVPVDRRQRQALVVGALTPLLPAHALLW
                     QREDTVPYECDGLTAYRELPLAVALPETEAQLAAVLAACHRLGVPVVARGAGTGLSGG
                     ATPHALGVTLSLAKFNRIVRVDPVSRTAVVQSGVRNAAISEAAARHGLYYAPDPSSKI
                     ACTIGGNVAENSGGMHCLKYGLTIHNVLRVRGYTIEGEAVEFGSEALDAAGLDLLSIV
                     IGSEGMLAVTVEVTVKLVPKPLLARAILASFDDVRKAGHAVAALIGAGIIPAGLEMMD
                     KPMTAAVEDYVHAGYDLDAAAILICESDGTPEEVEEEIGRMVAVLEDAGATRFDISRD
                     EAERLKFWSGRMNAFPASGRISPDYMCMDSTIPRNKLAEILVRIAEMEVTYGLRCCNV
                     FHAGDGNLHPLILFDANDPDQLHRCELFGADILETSVRLGGTVTGEHGVGIEKLSSMC
                     VQFTPAEREQMLAVKRAFDPTELLNPGKVIPTLHRCAEYGKMHVRKGLLPFPDLPRF"
     gene            complement(10926..12155)
                     /locus_tag="PKV56_04900"
     CDS             complement(10926..12155)
                     /locus_tag="PKV56_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOX67184.1"
                     /translation="MRLFLHAHATHPDWRMALSLATAQLEAQRAQPDHIDTPTLGWVY
                     LTDHFAAVAEHALEALREQWPGVAWVGSVGVGIAANGVEYFDEPALALMLGDIAPEHF
                     RVFSGVRPLSGQAGFAAATAQVHADPSGHDVSELISELADQTRNGYLFGGLASGRGRS
                     VHIADGVFEGGLSGVAFDDHVPLISRVTQGCQPIGAVRRISQAERNVVVSLDDEPALT
                     VLLRDLKVDAQDPREALPRLRGTLVGLSDPGRVSAHGRSFGVETRVRHLVGIDPGRNA
                     VAVADMVEPGMQLAFCQRNTEAARRDLVRICAEIREELEPEDLPLETALALHGSDLDS
                     APHPARQIAGAVYVSCSGRGGPHFGAPSAELAIVRHALGDVPLVGFFANGEIAHRHVY
                     GYTGVLTVFTAGQRPAE"
     gene            complement(12152..12862)
                     /locus_tag="PKV56_04905"
     CDS             complement(12152..12862)
                     /locus_tag="PKV56_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67185.1"
                     /translation="MTDIPSFTSFAPGFEFLQGLVKNAGSAIPGIGQWVAPTLNPEEL
                     DKRIQELRTIQFWLEQNARMLGATIQALEVQKMTLATLQTMNVQMSDLRDSLTIRMPG
                     MAAARGAPDAAAPADARPAAEPAAPPDEIPPTEAPAQAGSDASAAADAGTPKPAVDPM
                     QWWGALTQQFSELAANALKEGAAGLAAAPATTPAPAAGKSRPKATAPAQPRKAAAKTS
                     GGRKASTASRKTTPRSGR"
     gene            complement(12959..13447)
                     /locus_tag="PKV56_04910"
     CDS             complement(12959..13447)
                     /locus_tag="PKV56_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOX67186.1"
                     /translation="MSQDLPPTFKVVTLWLLLGLGVFLGIQWWLHAQQQTRFRSDGGM
                     IEIARGPDGHYHWPGRINGREVDFLIDTGATSTAISAALAQELQLDALGRVQSNTAAG
                     VVSGQVVSGNVTLDGGVVAERLRITALPGLGERPLLGMDVLGRLRWTQEAGVLRIDLC
                     PR"
     gene            13616..14755
                     /locus_tag="PKV56_04915"
     CDS             13616..14755
                     /locus_tag="PKV56_04915"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOX67187.1"
                     /translation="MRSDNPPNTPSGRPPATVLASLTLMALALHGCLLDAVPVEAAEL
                     SSPVARTAPAVQVRSLVSPPIEPGAQAALQPAAGPMPPVLVDPFVPLAPSRLAEIEPA
                     REAPPEPPTAGAPEAVLSSELSPSAPAMEAAPLASRPEARAPAPAASEPAVLRQPEQP
                     VPVYRTRIPPAMTIEYDISRGSISGTGQLTWLPEGGSYEARLQASVIGYTLLSQISQG
                     GFDKAGLAPLRFTDQRARKAARAANFQRDAGKITFSGPADEFALPAGVQDRLSWMIQL
                     PSVLSAEPKRAVTGGEVVLYVVGARADVAVWTLRCIGAETVQTAGGPVRTVKFMRAPR
                     GAKDTQAEVWLDPTRHFLPVRARLGQGSDDPDAFELVLRELKPGP"
     gene            14909..15193
                     /locus_tag="PKV56_04920"
     CDS             14909..15193
                     /locus_tag="PKV56_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HOX67188.1"
                     /translation="MHMLYNSDSFVVMQFEVPAEAGAPEAAHTLMRGGYEIVDKFACK
                     EIFIEGAMAESFREGVDALIDTRPAGPTEDEMDAYIERYSSLMQHPVVLH"
     gene            15295..17637
                     /locus_tag="PKV56_04925"
     CDS             15295..17637
                     /locus_tag="PKV56_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67189.1"
                     /translation="MVHSLDEVRVVLSPGLGDAPVLDRMCSQFVLTLTMRHVGRFNPR
                     RDWNSLLSLTGRHLVWPVSVLSRLRDFLAARCRGNELWRGHEALSDEVFIERHGNWRG
                     PYEEVTLFFYVDEYIKDAPKDLLAVLGATADWLERSLKKESTLVEKNIDALAGLLQLN
                     PAERALLLYGTLARYQRDLRGLLVEFKVSSAQEAHAAIASVAGVNEADVADALRAGSR
                     LERIGMVENLISEHNITDLADLMKVSEQLPPVLMRKYEGPSDLMAVFTRPVRRSELLP
                     SDFHYVSEDRDVLTALLSHAVGRREHGVNVLLYGPPGTGKTELAKVAAQAAGLELYEV
                     EYADRDGNSLNGRDRYRSLQISQVFLKASASVALLFDEVEDVFPPISTDAAQLMARID
                     TGDGAPSGSVSGKAWVNQILETNPVPVIWVTNRIEQIDPAFRRRFQYHLELKSPPPGA
                     REALVTRALAGVPVSAGFVARLAERRGLTPAQVRTAVKFARLATPADALLDAVSAEPG
                     THSVAFEALIERQIGNADKALGADVQGRAPRSVVTSYDLGLVNTESRFEVPKIVEALR
                     RKACGTLCFHGPPGTGKTALAEHIAQQMQRPLLVRQASDIMSKFVGETEQNMARMFEE
                     AQLEQAVLLLDEADSFLRSRRMAERNYEISEVNEMLQGMERFAGVFICTTNLFGELDE
                     AALRRFTFKVRFKPLTAEQRERMFVAEALGGDPARLTAEQQARLLELESLAPGDYASV
                     KRQIDVLGEAFEPDEFLSQLEAEHRVKPEVRQQRHIGFMH"
     gene            17702..19804
                     /locus_tag="PKV56_04930"
     CDS             17702..19804
                     /locus_tag="PKV56_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX67190.1"
                     /translation="MIELFVQSLFALDWKLAFLAAFVCLDLARRLESKLDLAGWGWLL
                     GASLALATGLWSAQVIGIERLQLPYAIEYRPGPAFGVWLGAGAISALGLALACRRSSG
                     LSVTLIGALLLAAGIVAIQMCFVEALWLQPGPAWRLPGLGMALVAASAGCAAALWLFA
                     EIRVRAREGALAWQAASALVLGLSIVISQGVVMGAAGLESPHVSVVTQDFLSEDTMAV
                     LVKVVSAALLSMLLVLSQIEAHLRASLQRAKGELRQQSLRDPLTELPNRQIFEGTLAQ
                     AARQADADQGQLALMLINLDGFKPINESLGHRVGDRVLREIAARLRSLARVHMVARLG
                     ADEFLLLMPDNPTTEQVSDLAARVLGSLSLPCPIDGRETTVTASIGIVMYPEHGAVST
                     LVAHADAAMRFAKSSGGATYCFFEARMVSGLREQTELLRDLRRALAQGELELFYQPKI
                     HAPTGEITGAEALMRWQHPRLGMISPGVFIPIAERFGLIGSLGNWLIDEVCRQIRVWR
                     DGGLRMRVAINLSVHQLRQPDLVERIARALREHDVNPGLLTCEITESVAMQDAEGTIK
                     FFGQLAQLGVSISIDDFGTGHSSLAYLRKLPASELKIDRSFVLDLETSEDARKVASAV
                     VNLAKALNLKVVAEGVETEGQNRILREFGCDQLQGYLFAKPMSAKSLALWAMDDVGPR
                     SISFRSSLFLETQPAELV"
     gene            19851..20912
                     /locus_tag="PKV56_04935"
     CDS             19851..20912
                     /locus_tag="PKV56_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOX67191.1"
                     /translation="MKTQPYTRGAQLPDLLRQRIVILDGAMGTMIQRYKLGEADYRGE
                     RFKDHPRDLKGNNELLLFTRPDVISEIHDQYLAAGSDIIETNTFGATRIAQDDYGLAS
                     VAREMNVVGARLAREACDRYSTADKPRFAAGALGPTPRTASISPDVNDPAARNVSFDE
                     LKDSYLEQAAGLLEGGCDLFLVETIFDTLNAKAAIFALDELMESTGERLPVIISGTVT
                     DASGRILSGQTVTAFWHSVRHARPLAIGLNCALGATLMRPYIEELSKVAGDTFISCYP
                     NAGLPNPMSDTGFDETPEITGRLVEEFAKSGFLNIAGGCCGTTPAHIAQIAQRVGAYR
                     PRCLHERPFSSLLESTAAA"
     gene            complement(20937..22157)
                     /locus_tag="PKV56_04940"
     CDS             complement(20937..22157)
                     /locus_tag="PKV56_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOX67192.1"
                     /translation="MAVVPISASAIRINQPVPFALRDAAGLMLVPRGGVVPSESVRKQ
                     LTERGVYIDISDADAFKKALAGKMDSMVRDNARIGRIAQAEAEVDPDSVMDNFGVPER
                     ARRGDTSLTWDGLVMKMAAALREPSAEDFIDRIDRIDEDLLSLISRGADAALLSLIYS
                     ATTDSQKYSVRHALLVTTIVELAARHLNGWPREWKAALRRASLTMNIAMTNLQDQLAV
                     QDSATTPQQRELIDSHAARGVAQLRELGVADELWLQAVEHHHTSPPGPLQAMEPGLQI
                     ARLIKRADVFAARLSPRKTRKAMTATAAAKAAYLDENQQPDEAGAAIIKAAGIYPPGC
                     FVRLAGGEVAIVLKRGLRASIPVVACVVGINGIPMSKPAVRDTRHKAYEITGSAAPHE
                     VKVRLSVESLIELM"
     regulatory      22270..22362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            22432..25176
                     /gene="metH"
                     /locus_tag="PKV56_04945"
     CDS             22432..25176
                     /gene="metH"
                     /locus_tag="PKV56_04945"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOX67193.1"
                     /translation="MLPVPSTPPSIVEPMKLSGLEPVLIGNGSLFVNIGERTNVTGSK
                     AFARMILNGDFEQALSVARQQVENGAQIIDINMDEAMLDSQAAMVRFLNLIASEPEIA
                     RVPIMIDSSKWSVIEAGLKCIQGKGIVNSISLKEGVEQFRHQAKLVRRYGAAAVVMAF
                     DEKGQADTYQRKTEICERAYRILVDEVGFPPEDIIFDPNIFAIATGIEEHDNYAVDFI
                     NATRWIKQHLPGAKVSGGVSNVSFSFRGNEPVREAIHTVFLYHAIRAGLDMGIVNAGM
                     VGVYDDLAPELRERVEDVVLNRRPDSTERLLEIAENVKGAAKDDSQRLAWRSGTVDER
                     LSHALVHGITDFITEDTEACWQRIAATGGRPLHVIEGPLMAGMNVVGDLFGQGKMFLP
                     QVVKSARVMKQAVAHLLPYIEAEKLALVEAGGEAKAKGKIIIATVKGDVHDIGKNIVT
                     VVLQCNNFEVVNMGVMVPCHDILARAKVEGADIIGLSGLITPSLEEMQHVAAEMQRDD
                     YFRVKKIPLLIGGATCSRVHTAVKISPHYEGPVVYVPDASRSVGVCSDLLSDEKAAAY
                     IAELKSDYDKVREQHANRKATPLVMLAEARANKVPIDWVNYAAPKPKFTGRRVFRNYD
                     LAELAQFIDWAPFFQTWDLAGAYPAILTDDIVGESARRVLSDGQRLLKRLIDGRWLTA
                     NGVIGLYPANTVGDDDIEIYTDETRSKVLFTWRGLRMQSVRPVVDGVMRPNRCLADYV
                     APKDSGVADHIGLFAVTSGIGAEKKEKQFLDDHDDYSSIMLKALADRLAEAFAERMHQ
                     RVRTEFWGYSAEESLTPAELIAEKYRGIRPAPGYPACPDHTVKGAMFEALNCADIGMA
                     LTESWAMSPAASVSGFYIAHPDATYFNVGKVGEDQIEDWARRTAQDAAQVRRSLAALL
                     "
     gene            complement(25201..26736)
                     /locus_tag="PKV56_04950"
     CDS             complement(25201..26736)
                     /locus_tag="PKV56_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOX67194.1"
                     /translation="MALALVHSRALDGLIAPPVTVEVHLANGLPSFTLVGLADTEVKE
                     ARERVRAALQNSGLEFPHNKRITVNLAPADLPKESGRFDLPIALGILAASGQIDAQRL
                     AQAEYAGELSLAGDLRPIRGAIAMALALKRGFSERALVLPQTSAQEAALVPGLRIQGA
                     MHLLDVVRSLLPVDAELRVELPLACSLLATRGEQPQAAAIDLRDVKGQAAAKRALEVA
                     AAGGHSVLMTGPPGTGKSMLAHRFCGLLPALSEAQALESAAVISLNGSAFDASQWARR
                     PYRSPHHSASSVALVGGGSPPRPGEISLAHEGVLFLDELPEFPRMALEALREPLETGR
                     IVISRAARQAEFPARFQLLAAMNPCPCGHHGSTLRSCRCSPDAVSRYQGRISGPLLDR
                     IDLQVEVPALSADALAAAPDGEPTAVVAARVAVAQQLQHDRQGNLNSTLWGEALDIHC
                     RLDDAASRFLTAAASRLAWSARSHHRVLRMARTVADLAASESIQVAHLAEAIQYRRVL
                     AAR"
     gene            26966..27760
                     /locus_tag="PKV56_04955"
     CDS             26966..27760
                     /locus_tag="PKV56_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HOX67195.1"
                     /translation="MNKTQIALATALLASVSTLTHAADAAPAPAPTPDWTITGNAALS
                     SDYRTRGISQTDRRAAFSGGFDVAHSSGFYIGNWNSNIDSDFYTGANLELDFWAGYKG
                     SVEGIGYDIGVFYYYYPGSDRQGAAGIDNKEIYVGTSYGPVSARLYIPVDDFFSAQRQ
                     NFGNGKNAAGSYYLDVAGSYDLGNSFGLIGHFGYQKLKGAAKLQEIGKSKFENDIIDW
                     KLGVTYTFSNGFVATVTYIDTDIKIAGPSGKKISGPTGVFSISKTF"
     gene            27789..28127
                     /gene="glnK"
                     /locus_tag="PKV56_04960"
     CDS             27789..28127
                     /gene="glnK"
                     /locus_tag="PKV56_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOX67196.1"
                     /translation="MKMVTAIVKPFKLDEVREALSAIGVQGVTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVDDSIVDRVIEAIENAARTGKIGDGKIFVSSLEQVVRI
                     RTGETGKDAL"
     gene            28153..29697
                     /gene="amt"
                     /locus_tag="PKV56_04965"
     CDS             28153..29697
                     /gene="amt"
                     /locus_tag="PKV56_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOX67197.1"
                     /translation="MKKLIATLALGLAVLGFSVATFAQDGAASAPAVAEAAVMATEAA
                     SAAAEAVAPAPAEAAAATEAPAAAPPVPNKGDTAWMMVSTIIVIMMAIPGLALFYGGL
                     VRSKNMLSVLMQVMVTFSMIVVLWFVYGYSLAFTEGNAFFGGLDRLMMKGVWDNAAGT
                     FTNAATFSKGVYIPEIVFAVFQASFAAITCALIVGAFAERAKFSAVLLFMALWFTFSY
                     APMAHMVWFWMGPDAYSSKEVVDEMTSKAGYIWQSGALDFAGGTVVHINAAVAGLVGA
                     YMFGKRVGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSALEANGFAALAFINTFGA
                     TAAAVLAWCAGEALMKGKASMLGAASGAVAGLVAITPACGNVGIGGALIIGVIAGFAC
                     LWGVSGLKKILGADDSLDVFGVHGVGGILGALLTGVFNAPSLGGPGYVADWVTVAMVT
                     PADYSIAAQVWIQAKAVLITIVWSGVVSVVAFKVVDLLIGLRVPEDEEREGLDITSHG
                     ETAYHQ"
     gene            29887..31182
                     /gene="gshA"
                     /locus_tag="PKV56_04970"
     CDS             29887..31182
                     /gene="gshA"
                     /locus_tag="PKV56_04970"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOX67198.1"
                     /translation="MVPHLITALTGPINELEQRILESLPAIERWFRLEWMEHTPPFYT
                     SVDVRNAGFKLAPVDTNLFPGGFNNLTAEMLPLAVQAAMAAIEKICPEAKNLLLIPEK
                     HTRNTFYLANVARLMQIFHMAGMNVRLGTLDDTITVPTPIELSDGTQLTLEPLIRNRG
                     RLGLKDFDPCTILLNNDLSAGIPKVLENLHEQYLLPPLHAGWAIRRKTNHFAAYEEVA
                     KKFAKLLGMDPWLINPMFARCGEVNFSDGTGSECLMSNAEALLAKVKRKYKEYGIQEK
                     PFIIVKADAGTYGMGIMTVRDPKELAELNRRSRNKMSVIKDGQEVSEVILQEGVPTYE
                     RVNDAVAEPVVYMIDRYVVGGFYRVNAERGIDENLNSPGASFVPLAFAESNQLPRPGV
                     KPGASAPNRFYMYGVIARLAMVAASYELEATDPDAEIYE"
     gene            31329..>32288
                     /locus_tag="PKV56_04975"
     CDS             31329..>32288
                     /locus_tag="PKV56_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOX67199.1"
                     /translation="MTPHRPASSLAALTLGAIGVVYGDIGTSPLYALKEVFAHGHVPL
                     TPDNIYGVLSLVFWTLSIVVSVKYVMLILRADNNGEGGLIAMLALASQAVREQPVLRR
                     RLLLLGIFGTAIFFGDGVITPAISVLSAVEGLEVAAPGLHRYVVPLTLAVLTLLFIFQ
                     RFGTGSVGRLFGPITVLWFAVLAALGAVHIAENPAILWALSPHHALGFMVSQPAIAFI
                     ALGSVVLCVTGAEALYADMGHFGKRPIRLAWFSLVMPALVINYFGQGAMLLLHPENVR
                     NPFYEMAPTWALYPLIGLATCATVIASQALITAAFSVTKQAIQL"
BASE COUNT         5273 a        10643 c        10933 g         5439 t
ORIGIN      
        1 ggcgcttcac cgacgcctgg ctgtcgatgg ccggcaccgc cgacatcggc atcccgaacg
       61 gcctgccggt cgacgaatgg ggcatccgcg tcgcggccga caagtgcacg ccggtcggtg
      121 cctcggtctc gcgcggtggg gccaccaact cgccggccgc cgtctacgcg ctcaccaagt
      181 acgtcgactg gatgaagaag tacgcgccga aggacgcctc cggcatgacc ttcggcgagg
      241 cgggtccggt gccggcgcag ggccagatcg cgcagcagat cttctggtac accgccttca
      301 ccgcggacat gatcaagccg ggcctgccgg tcgtgaacgc cgacggcaca ccgaagtggc
      361 gcatggcacc gggcccgaac ggcccgtact ggaagcaggg catgcagaac gggtaccagg
      421 acgtcggttc ctggaccttc ttcgccgacc atgacgccga caagaccgct gcagcgtggc
      481 tgtacgcgca gttcatcacc tcgaagacgg tgtcggcgaa gaagagcgtg gtcggcctga
      541 ccttcatccg cgacagcgac atccggcacg actacttcac caagaacgca gccaagtacg
      601 gcggtctgat cgagttctat cgcagcccgg cgcgggtggc ctggacgccg accggcacca
      661 acgtccccga ctatccgaag ctcgcgcaac tgtggtggaa gaacgtggcc gtcgccgtca
      721 ccggcgagaa gacgccgcag gccgcgatgg acaacctggc cgaagagatg gatcaggtga
      781 tggcccgcct cgagcgagcc ggcatggccc attgcccgcc gaagctgaac ccgaagggcg
      841 acccggccaa gtacctgagc gacaagggcg caccgtgggc caagctggcc aacgagaagc
      901 cgaagggcca gaccattggc tacgacacgc tgctgagcgc gtggaaggcc ggcaaggttc
      961 gttgagcctg ccgcgcggcg ccgaccctag gcggccgtga aggctcgggc gccgtccccc
     1021 gtcaatgcga cggcgggcgg cgccacgagc agcagctcgc cgcccagcgc atcgatcagc
     1081 tcggggatca gctgcaccag ctcgccggtg gcgatggcgg cgtccgcatc gaaggcgtcg
     1141 tcccggctgt cgcggctggg gcggccctcg aacaccacgt cgaggaagtc gatcttcttg
     1201 atctgcccgg tctcggtcag cacgaacgac acgcggccct gccaggtcag cgccagccgg
     1261 gtcggccgct tgccgctggc gatgtgcccg cgcacctcgt cgatgtcgag cggatggcgc
     1321 gcgtagcgca ccaccgattt catttcgtct tccgatttca gttcgcactc gcggtcgacc
     1381 gaaaaggagg cgggtggctc gcccgtcagc agccagtccg acatcgcgac cgacgccgac
     1441 tcggtggtct gcagctgcgt cagcgtcagg ccgtcgagcg acttgatcag ttgcgtgacc
     1501 acctcgccgg cccgcgccgg gctgctggcg tcgatcatca gcaaccgctg tttcgcatcg
     1561 atccatacct tcagcgtcga gcgcttcgtg aaggccatcg gcagcagttc gagcaccgcc
     1621 tgttccttca gctccttcgt ctgcttcttg cccggtttgc ggccggtgct gtgctcgacc
     1681 tgctgcgcca tctcgtcggt gcggcgcttc accaccgcag agggcaacgc cttgcgctcg
     1741 atcatcagcg tcatcagcca ttgcccgccc accgattcga ccagcgggcc gtgctcggtg
     1801 ccccggggcg cgacccagcc ggccgactgc ggctgcgtgg cgccgcattc cgcgaaacgg
     1861 gcggccgcca ggccgtcctc gatctgctcc agcgtggccg accactcggg gcccacgcga
     1921 tacaccgtca ggttcttgaa caagcgaaag gtctttccat gtgaatgcgc tgcggcgtcc
     1981 gctgctcgcg gtctgccggg gaggcgggat tgtccacggc gagacggccc ggcttgcacc
     2041 ggcgaggctg cgtatgatcc gcccgcctct ccgcgactcg gtcgccctcc gtcaggactc
     2101 catggaatct ctcttcgtct cgaccggtgt cgtcgcactc gctgaaatcg gcgacaagac
     2161 ccagttgctc gccttcatcc tggccgcgcg cttcaagaag ccgctgccca tcatcctggg
     2221 catcctgtgc gcgacggtcg tcaaccacgg tctggccggc gcgctgggcg cctggatcac
     2281 cacgacgatc agccccgaga tgctgcgctg gatcctgggg gcctcgttca tcggcatggc
     2341 gatctggacg ctgattcccg acaagatcga ggaagaggaa acccagatcg ccagcaagct
     2401 cggcgtgttc ggtgccacgc tggtcacgtt ctttcttgcc gagatgggag acaagacaca
     2461 gatcgcgacc gtcgcgatgg cggcgcatta cccgaacccg gtgctggtgg tgatcggcac
     2521 gacgctgggc atgctgctgg ccgacgtgcc ggcggtgttc gtcggcgaca agctcgccag
     2581 caagatcccg atgaagctgg tgcactcgat tgccgcggcc atcttcgcgg cgctgggcgt
     2641 ggcgaccctg ctcggggccg gctcgaagct cggtttctga tcgccgcggc gatcgggcag
     2701 cgggtgattg ccctgcctga aagagggctt gcgatgttct agagtcgcgg ctgcttcccg
     2761 gctgtcaggg cgatgccggg ctgcccgacc gacccgcccg cagacactgg agacctcgat
     2821 gaacgacaaa ctgaagcagg ccgccgacac ggtcctcgcc aacaccgtgg gctgcgccgg
     2881 cggtgcgccc ggtgtggtcg cgatggtgac cgatcgcaag accaatgtgt acgaaggcgc
     2941 cgccggccgg cgctcgctgg gcggcgatgc accgatgacg accgactcgg tgatggcgat
     3001 cttctcgacc agcaaggcga tcaccggcac gacgctgatg cagctcgtcg aagagggcca
     3061 ggtccgcctc gacgatccgg tcaggaagta cgtgcccgag atcgccgaga tccaggtgct
     3121 cgagggcttc gacgccgccg gccagccgaa gctgcgcgcg cccaagcgcg acatcaccat
     3181 caaccacctg atgctgcaca ccgccggctt cagctacgag ttcttcagcg ccgacgacct
     3241 gaagctgcgc ggcgccaagg gcattccgtc ggtcgtgtcg agcaccttcg cgtcggtgaa
     3301 gacctgcctg ctgtgggacc cgggtgagaa gtggggctac ggcgtcaaca tcgactgggt
     3361 cggcaaggtc gtcgaagcgg tgcgcggcaa gcgcctgggc gacgtgatgc aggaacgcgt
     3421 cttcgcgccg ctcggcatga atgacacgag cttcgtgatg acgccgtcga tgcagtcgcg
     3481 ccgcgctgtc atccacgacc gcgcgatgga cggcaagctg accccgctgc ccgacctggt
     3541 gctgccgcag ccgcccgaga tggacatggg cggccacggg ctgtactcga ccgtcggcga
     3601 ttacatgaag ttcattcgca tgatcctcaa cgacggggcc gggccgaacg gccgcgtgct
     3661 caagacggaa accgtcgacc agatgtcgcg caatggcctt ggcgcgctga agtcgggcgg
     3721 ctggaccacg tcgatcccgt cgctgtccaa taccggcgag ttcttccccg gactgccgaa
     3781 gtcatggggc tacaccttca tgatcaacga cgaggcgaca ccgtcgggcc gcccggccgg
     3841 ttcgctgatg tgggcgggtc tcgccaacct gttctactgg atcgaccgcg agagcggcat
     3901 cggtggcttc tggggctcgc agatcctgcc gttccaggac gtgtcctcgt acccgggctt
     3961 cgtcgatttc gagacggcgg tctaccgcca tctgaagcgc tgaggcgttt caggttctgc
     4021 gggcgcgtcg ctggagcggg gcgcccgcct tcatcgaact acctcaccgt cgttgtgtat
     4081 ctcagactgt tcggtttgct gatgtcgaac gaaaacacct tgttgtcgtt ggaggcgatg
     4141 aaaaatcccc aacccacgac gcttctggcg gcaaccccgg ccccaaagct ctgtggcccg
     4201 ggcgcgagaa taccgtcggg ttcgactcca tcagcgtagg tgtccgcata gtcggccgtg
     4261 ccgggcaatg ggccgagtgc ggtggcttgg cgcgtcctgg tgtcgtagac cgtgagcgtc
     4321 gcggcataga agtcctgcgc attgttggcg gcaaagaaga agcgttcggc atggacactg
     4381 cgtccgaagt tcacggtgtt cgtgtcctcg tgcgcaccga ctccgtagcc ttggtcggct
     4441 gtgtagagcg tgttgttgtc ttcgtcgatg aactgaagca cgcgccgctc gccggctggc
     4501 agtagcgaga acgagttctg gtcctccagt tccagcatgt gcacccccgc agcgcctggg
     4561 tggatggtgg gcgtgatgcc gatgggatag gccgttttct gaaccgagcc atcggtcttg
     4621 ctgatacgga tcaagtttct gtgcgtcgcc ctcggcccac ctccggccat tccagggatg
     4681 gtgagcgata tggcgtaaag cgcgttgctg cccaaccgca gttcgctgat cgagtccgtt
     4741 ccactgacaa gcaccgatgc gaccgggctg gatcgcgtga tcttcaggac tttccagaca
     4801 tcggagcgcg tgctcggatc aaacgaagag gcgcccaggt agtacgcgtc gtcgtcgaag
     4861 atgaatgtcc cccggtagaa ggggccgaag ccggcggtga cggccgggtt cagtggcgtc
     4921 tcgatgagcg tgctccccga aggaaagtcg agcacgctca gcccgcccgg gcgctgaacg
     4981 agtgcctggc gatcggtgct ggcaagcacg atttgcgtgt cggtggtgcc gggcgggctc
     5041 agcgggatgg tggtgccgtc ggcccacagc accacgcggt cgcgatacag ccaggcacgg
     5101 ggtgccagcg tggctgtatc gcgcaggacg ccgactggga tcgggtcggc cggggtcgtg
     5161 ctcaagacgg tggaaaccag ctcgccgctg gcggaaaacc gcaactccag caggccttca
     5221 tacacgtcgt ctgggttgtc cgattcattg aagcagttga tgccccctcg gctcctgcgc
     5281 ggcacgatga ggcgtgagtt cagcggattc gtgtaatcgt ttcccaccac acccaggttg
     5341 ttgcatgact cggccaggcc cgacgaggga ggcttcgaca cgggcgccgc cccatgggct
     5401 tccagcgaca cgcttcgcac actctggcct gcgtcgtaga cgatcgtgtg ggcccgcacg
     5461 tccgtgacct gctgcgtgct ggcgttcacg gttccgctgg acacgatcag ccaccagcgt
     5521 gggccgtcgc tgctctgtgt gattgcatat tcggtgtccg gctggctcgg gtgaatcagt
     5581 gacagccgat caccgccagc tcccggcttg acgatccagc cataggtgcc ggaggtcggg
     5641 tagatcgacc tctcttgatc actccccccc ccgcaactcg acagtccgat cagcgtggaa
     5701 agcagggccg cgaggcagag ggtgcgtggc aggttcatcg attctccgaa caggtttgct
     5761 ttgtttgatg atcgctgatc agcaggtggg tgccgatccg gcctaccctg ccaacgcctc
     5821 gtccagcacc tccacccagt gcttcaccgg cgttggcgtg ccggtctgca ggtgctggat
     5881 gcagccgacg ttggccgaca cgatgacctg cgcctcgacc tcggcgaggt tggccagctt
     5941 gcggtcgcgc aactgatgcg ccagcgttgg ctgcagcacc gagtaggtgc cggccgagcc
     6001 gcagcacagg tggctctcgt tcgctgacag ccgaacgtcg aagcccagcg cgcccaggtg
     6061 cgtctcgacg ccgccgcgca gttgctgcgc atgctgcagc gtgcagggcg ggtggaaggc
     6121 gagccgtgcg gtcggcttgc gatggatgcg tggcttcagc agcggcacca gatcgggcag
     6181 caactcgctc aggtcgcgcg tcagctcgct gatgcgcttc gccttgtctg cgtaggcctc
     6241 gtcgtgagcg agcgcgtggc cgtagtcctt gacggtcgcg ccgcagcccg aggcgttcat
     6301 cacgatggcc tcgatcttgt tcgagatcac gctgggccac cacgcatcga tgttgcggcg
     6361 catgtcggcg agtccgcccg cctgatcgcc gaggtggctg cggatcgcgc cgcagcagcc
     6421 ggcttcctcg gccaccatcg tctggatgcc ggcggcgtcc agcacccggg cggtggcgct
     6481 gttgatgttg ggcagcatcg ccggctgcac gcaacccatc agcatcagca ccttgcgcgc
     6541 gtgttcgcgc ttgggccagc ggtgcgcgag ggcccgttcg cgcgggccgg gcgttgcagc
     6601 caccttgccc ttgagggctg cgggcagcag gggccgcgcg aactggccca gcttcagtgc
     6661 ggtgtcgaac agcggcgagg tcagcccttc cttcaacagc cagcgcaccg ccttctcgcc
     6721 gcgagggcgt tccaccttcc catcgacgat gcgccggccg atctcgacca ggttgccgta
     6781 ctgcacgccg gacgggcagg tggtctcgca gttgcggcag gtcaggcagc ggtcgaggtg
     6841 ctgttgcgtc ttgcgcgtgg gctcggcgcc ttcgagcacc tgcttgatca ggtagatgcg
     6901 accgcgcgga ccgtcgagct cgtcgccgag caactggtag gtcgggcagg tggcggtgca
     6961 gaagccgcag tgcacgcact tgcgcaggat cgcctcggcg gagtcgccgt cgggcgtgcc
     7021 tttgaattcg ggggcgagat tggtttgcat ggatggatac ggagtgtctc aggccggcag
     7081 cagccgcaac agcaaaggga tgaaccaggg cagcagcagc gaggccagca gcacctgcag
     7141 gcccagtgcc aacccggcat aggcgcccga gtcgggatgc acctgcagag cacgcgccgc
     7201 cccgatgccg tgggccgcgg tgccgatcgc gaagccgcgc acactgtggg ctgtgatgcc
     7261 cagcgcgtcg aacaggtact tggcgctgag cgcgccgacc agcccggtca gcaccgcgaa
     7321 caccgccgcc agcgcgggga tgccgccgag ttgttcggcg atgcccatcg cgaccggcgc
     7381 ggtcaccgac ttgggcgcca gcgaccgcag caactcggcc ggcagatcga gcgaccagcc
     7441 gatcgctacc gcgctgccgg ccgccgccag cccgcctgcg agtgctgcga cggtgaccgc
     7501 gagtgcgcgc tcgcgcagct ccgagcggcg ctgccaaagc ggccaggcca gcgcgaccac
     7561 cgccgggccg agcaggaagt ggatgaactg cgcgcccgag aagtacgtcg ggtagggcgt
     7621 gccgctgagc gtcagcagca gtgccagcgc gatcaccgtc cacagcacgg gattggccca
     7681 cggcgcctgg ccaagacggc tgtagacgcc ctgggcgatc acgtagatca ccagggtcgc
     7741 ggtcagcccg aacagcggcg tggccgacag gtagacccag agttcgacga agttcggcat
     7801 cgtgcgcgcg gtcggcgggt cagcgcggag cggccgggtc gggcttcggc aggcccgttg
     7861 aagtggcctt tggcaacagg gcgcgcagca ccagtgccgt cacggcgagg ccgatccagg
     7921 tcgacagcag cagcgcgacc gccagccgcg cgccgtactg cgagaccagg gccatgtgcg
     7981 tcatcacgcc gacgcccacc ggcacgaaca gcagcgacag gtgcgccagc agcacgtccg
     8041 caatggcgcc gaccgggcct cgcaccggct cccagccgag tgccagcatg aggatcagca
     8101 tgccgatcac cgggccgggc agaccgatcg gcgcggcgcg tgccagcaat tcgccggccg
     8161 actggcaggc cagcagccag gccaggccct ggagcggcgc ggcggtcgat gtcacggacg
     8221 gtggtcggct cgctgcatca caacccgggg tacagccgtc ccggattgaa cagcccgtgc
     8281 ggatcgaacg agcgcttcag ttcgcggtgt atgcggtcca gtggcgagct cagcggtgcg
     8341 aacacaccgg cagccttgtc gagactgcgc agcagcgtcg cgtggccgcc ggcgcgtccg
     8401 gcgatctcgc gcaggtgggc ggcggtctcc gggctcgcgt ccggcgtcac gcaccagcgc
     8461 tgccccccgg cccactcgat cagcggtgtt ccgggcaggt ccagcatggg cgcggtctgc
     8521 ggtaccgaca ggcgccacag cgctccaccg gactgcaccg cggcctgggc gcccatgaag
     8581 aactcgtcct gatggtcgcg aagccccgcc cagaacggat cggccaggtc cgggccaatc
     8641 acttcgccac cgagcgccag gaactgcgat gtggccgtct gcaccgctgc agcagcacca
     8701 cgaagccgca cgacaagcat gccgttccag agtgcgctgg cattcagcgg cagcggctgg
     8761 cagccccatt gatgcagttg cgccagtgcg tgcgctgctt ccatgtcgaa acgcagcgtg
     8821 gtggtggccg gcgcgaccgg cagcaccttg atcgacacct cgaggatcac gcccagcgtg
     8881 ccgagcgagc cgaccagcag acgcgacacg tcgtagccgg cgacgttctt gatcacctgg
     8941 ccgccgaagc tcagcacctc gccccggccg ttgagcagcg tggcgccgag cacgtagtcg
     9001 cgcaccgcgc cgaccgccgc gcgcgacggg cccgacaggc cggccgcgat catgccgccc
     9061 acggtgccgc cctgggccgt ggcctcgggg gtgtcgcctg caaagtgcgg cggctcgaaa
     9121 ggcaggcact gccccttctc ggccagcgcc gcttccagtt cggacagcgg cgtgccgcag
     9181 cgcgcggtca ccaccagttc ggtcggcgca tagctcgaga tgccgctcag ctcggtcgtg
     9241 tcgagcagtt cgccctgcgg cagttctccg tagaagtcct tggtgccgct gccgcgaatg
     9301 cgcagcggcg tgccgcagtc gcgtgccgac cgcacgcgtt cgatcaggcg cgtgagcgca
     9361 gggctttcgt cttcgttttc gatcaaggca ggctcgctca gaagcggggc aggtcgggaa
     9421 atggcagcag ccccttgcgg acgtgcatct ttccgtactc ggcgcaacgg tgcagcgtcg
     9481 ggatcacctt gccggggttc agcagttcgg tcgggtcgaa ggcgcgcttg acggccagca
     9541 tctgctcgcg ctcggccggc gtgaactgca cgcacatcga gctgagtttc tcgatgccga
     9601 cgccgtgctc gccggtcacc gtgcctccga ggcgcacgct ggtctcgagg atgtcggcgc
     9661 cgaacagctc gcagcggtgc agctggtcgg ggtcgttggc gtcgaacagg atcagcgggt
     9721 gcaggttgcc gtcgccggcg tggaacacgt tgcagcagcg caggccgtag gtcacctcca
     9781 tctcggcaat gcgcaccagg atctcggcga gcttgttgcg cgggatggtc gagtccatgc
     9841 acatgtagtc ggggctgatg cgaccgctgg ccggaaacgc gttcatgcgg ccgctccaga
     9901 acttcagccg ttcggcttcg tcgcggctga tgtcgaagcg tgtcgcgccg gcgtcctcga
     9961 gcaccgcgac catgcggccg atctcttcct cgacctcttc tggcgtgccg tcgctttcgc
    10021 agatcaggat cgctgcggcg tcgaggtcgt agccggcgtg cacgtagtcc tcgaccgcgg
    10081 cggtcatcgg cttgtccatc atctccaggc cggccgggat gatgccggca ccgatcaagg
    10141 ccgcgaccgc gtggcccgcc ttgcgcacgt cgtcgaagct ggccaggatc gcgcgcgcca
    10201 gcagcggctt gggcaccagc ttgaccgtca cctcgacagt gaccgcgagc atgccctcgg
    10261 agccgatcac gatcgacagc aggtcgagcc ccgcggcgtc gagcgcctcg gagccgaact
    10321 cgaccgcctc gccttcgatg gtgtaaccgc gcacgcgcag cacgttgtgg atcgtcagac
    10381 cgtacttcag gcagtgcatg ccgccggagt tctcggccac gttgccgccg atcgtgcagg
    10441 cgatcttgct gctggggtcg ggcgcgtagt acaggccgtg gcgcgcggcc gcctcgctga
    10501 tcgcggcatt gcgcacgccg ctctgcacga cggcggtgcg gctgaccgga tcgaccctca
    10561 cgatccggtt gaacttggcg aggctcagcg tcacgcccag cgcgtgcggc gtggcccctc
    10621 cgctcaggcc ggtgccggca ccgcgggcga ccaccggcac gcccaggcgg tggcaggccg
    10681 cgagcacggc ggccagctgc gcctcggtct cgggcagcgc gaccgccagc ggcaactcgc
    10741 ggtaggcggt cagaccgtcg cactcgtagg gcacggtgtc ctcgcgttgc cagagcagcg
    10801 catgcgccgg cagcaacggg gtcagggcac ccaccaccag ggcctgacgc tgccgacggt
    10861 cgaccggaac atcgggctgc ggcgcgggcg acaactcggc tgccgtggaa ggagcattca
    10921 tcgtttcact ccgcagggcg ctggccggcg gtgaagacag tcagcacgcc ggtgtagccg
    10981 tagacatggc ggtgcgcgat ctcgccgttg gcgaagaagc cgaccagcgg cacgtcgccg
    11041 agcgcatgcc ggacgatcgc cagctcggcc gacggcgcac cgaaatgtgg tccgccgcgg
    11101 cccgaacagc tgacgtagac cgcgccggcg atctgccgag ccgggtgcgg cgcgctgtcc
    11161 aggtcgctgc catgcagcgc cagtgcggtc tcgagcggca ggtcctcggg ctcgagctct
    11221 tcgcggatct cggcgcagat gcgcacgagg tcgcgtcgcg cggcttcggt gttgcgctga
    11281 cagaaggcca gctgcatgcc gggttcgacc atgtccgcga ctgccacggc gttgcgccct
    11341 ggatcgatgc caaccagatg ccgcacgcgc gtctcgacgc cgaagctgcg gccgtgcgca
    11401 gacacccttc ccggatcgct caggcccacc agcgtgccgc gcaggcgagg cagggcctcg
    11461 cgggggtcct gcgcatcgac cttcaggtcg cgcagcagca cggtgagcgc cggctcgtcg
    11521 tcgagcgaca cgaccacatt gcgctcggcc tggctgatgc gtcgcaccgc cccgatcggc
    11581 tggcagccct gcgtgacacg cgatatcagc ggcacgtgat catcgaaggc cacgcccgac
    11641 aggccaccct cgaacacgcc gtcggcgatg tggacgctgc gcccgcggcc cgatgccagt
    11701 ccgccgaaca ggtagccgtt gcgcgtctgg tcggccagct ccgagatcag ctcggagacg
    11761 tcgtgaccgg agggatcggc gtgcacctga gcggtcgcgg ccgcgaagcc ggcctgaccc
    11821 gacaacggcc gcacgccgga gaagacgcgg aagtgctccg gcgcgatgtc gcccagcatc
    11881 agggcgagcg caggctcgtc aaagtattcg accccgttcg ccgcgatccc gacgccgacc
    11941 gagccgaccc aggccacccc tggccactgc tcgcgcagcg cttcgagggc atgttccgcc
    12001 accgcggcga aatggtccgt caggtagacc cagcccagcg tcggcgtgtc gatgtgatcg
    12061 ggctgcgccc gctgtgcctc gagttgtgct gtcgccagcg acaacgccat gcgccagtcg
    12121 ggatgcgtgg cgtgggcgtg caggaagagt ctcatcgccc gctgcgggga gtcgtcttgc
    12181 gggaggccgt gctcgccttg cggccgcctg aagtcttggc ggcggctttt cgcggttgcg
    12241 ccggggccgt cgctttcggg cggctcttgc ctgccgcggg cgcaggagtt gttgcggggg
    12301 ccgcggccag cccggcggca ccctccttca gcgcgttggc ggccagttcg ctgaactgct
    12361 gcgtcaatgc tccccaccac tgcatcgggt cgacggccgg cttgggcgtg cctgcgtcgg
    12421 ccgcggccga cgcatcactg ccggcctggg ctggcgcctc ggtgggcggg atttcgtccg
    12481 gcggtgccgc aggctctgca gccggccgcg catcggcagg cgccgcggcg tcgggtgcac
    12541 cccgggctgc cgccattccc ggcatgcgga tcgtcagtga atcgcgcagg tcgctcatct
    12601 gcacgttcat cgtctgcagg gtcgccagcg tcatcttctg gacttcgagc gcctggatcg
    12661 tcgcgcccag catgcgggcg ttctgctcga gccagaactg gatcgtgcgc agctcctgga
    12721 tgcgcttgtc gagttcctcg ggattcaatg tcggcgcgac ccactggccg atgccgggaa
    12781 tcgccgaccc ggcgttcttc accagaccct ggagaaactc gaaaccggga gcgaacgagg
    12841 tgaaagaggg gatgtcggtc atggtgtgtc tcctgaacgc gaagccgatc cattgtgccc
    12901 gtcgaggccg cgcacgttgc ggtgtccggg agcagccctg ctgtcggctt gcgggcgctc
    12961 aacgggggca cagatcgatg cgcagcacgc ccgcctcctg cgtccagcgc aggcgaccga
    13021 gcacgtccat gccgagcagc gggcgctcac ccaatcccgg cagtgccgtg atgcgcaggc
    13081 gttcggcgac cacgccgcca tccagcgtca cgttgccgct gaccacctgt cccgagacca
    13141 cgcctgccgc ggtgttcgac tgcacgcgac ccagtgcgtc gagttgcagc tcctgcgcca
    13201 gtgcggccga gatcgctgtc gaggtggccc cggtgtcgat caggaagtcg acctcgcggc
    13261 cgttgatgcg ccccggccag tggtagtgcc catcgggtcc gcgggcgatt tcgatcatgc
    13321 cgccgtcgct gcggaagcgc gtctgctgct gcgcgtgcag ccaccactgg atgccgagga
    13381 acacccccag acccagcagc agccacagcg tgaccacctt gaaggtgggc ggcagatcct
    13441 ggctcatgga cgcgacttca tcggaacagg aagcatgggg tatgcgcgaa tgtccttgcg
    13501 gtgagctgtc gggggctggg cctcggcccc tcctgaatgg ggtgtgctgt aagccgggat
    13561 cgaaccatcg tcgtattctc ggctccctcc ttcttgttga ctgctgcgca agaccatgcg
    13621 ttccgacaac ccgccgaaca cgcccagcgg tcgcccgccc gccaccgtgc tggcctcgct
    13681 gaccctgatg gcgctggcac tgcacggctg cctgctcgac gctgttccgg tcgaggccgc
    13741 cgaactgagc tcgcctgtcg cacggaccgc gccggcggtc caggtgcgct cgctggtgtc
    13801 tccgccgatc gagccgggcg cgcaggctgc actgcagccc gcagctggcc ccatgccgcc
    13861 tgtcctggtg gatccgttcg tgccgcttgc gccctcgcgt ctggcggaga tcgagcctgc
    13921 ccgcgaagcc ccgcccgagc caccgaccgc cggcgcgccc gaggcggtcc tgtccagtga
    13981 gctttccccg tctgcgccgg cgatggaggc cgcgccgttg gcgtcgaggc ccgaggcccg
    14041 cgccccggct ccggcagcca gcgagccggc ggtgctgcgc cagcccgagc agcctgtgcc
    14101 ggtgtaccga acgcggattc ccccggcgat gacgatcgag tacgacatca gccgagggtc
    14161 catttccgga accggccagc tgacgtggct tcccgagggg ggcagctacg aagcgcgtct
    14221 gcaggccagc gtgatcgggt acacgctgct cagccagatc agccagggcg ggttcgacaa
    14281 ggccggactg gcccccctgc gcttcaccga ccagcgggcc cgcaaggccg cgcgcgccgc
    14341 caacttccag cgcgatgccg gcaagatcac gttctccggc ccggcagacg agttcgcgct
    14401 gccagcgggc gtgcaggatc gtttgagctg gatgatccag ctgccctccg tgctgtcggc
    14461 ggagcccaag cgggcggtga cgggcgggga agtcgtgctc tacgtggtcg gcgcgcgcgc
    14521 cgacgtggct gtctggactt tgcgctgcat cggtgccgag accgtccaga cggccggcgg
    14581 tccggtgcgc accgtcaagt tcatgcgtgc gccgcgcggt gcgaaggaca cgcaggccga
    14641 ggtctggctc gatccgaccc gacatttcct gccggtgcgc gctcgtctgg ggcagggcag
    14701 cgacgacccg gacgcgttcg agctggtgct gcgcgagctg aagcccggcc cctgagccga
    14761 ggggttgacc acaccgagcg agccggtgaa cgcttgaaac aagccttgtc aggcgcatgt
    14821 agggatggac cggcctgaaa gggcaggaac gcttcctgcg tgtgtggcac agccctgcag
    14881 gaatcgaccc aacccaagga gtttcccgat gcacatgctc tacaactcgg acagttttgt
    14941 cgtgatgcag ttcgaagtgc ccgccgaagc cggggcgccc gaggcggccc acaccctgat
    15001 gcgagggggc tacgagatcg tcgacaagtt cgcgtgcaag gaaatcttca tcgaaggggc
    15061 catggccgag agcttcaggg aaggggtcga tgcgctgatc gacacgcgtc cggccggacc
    15121 caccgaagac gagatggatg cctacatcga gcgctactcg tcgctgatgc agcacccggt
    15181 cgtgctgcac tgacaaggta cctccccctc ggctccgggg cttgatgccg gagtcggccg
    15241 agggcaagat cgcttcgatc ggtgcatccc gggcgccgtg ctccaggggt gttcatggtt
    15301 cacagtctcg atgaggtgcg ggtggtgttg tcgccaggcc tcggcgatgc gccggtgctc
    15361 gaccgcatgt gctcgcagtt cgtgctgacg ctgaccatgc ggcacgtcgg gcgcttcaac
    15421 ccgcggcgcg actggaacag cctgctgtcg ttgaccgggc ggcacctggt ctggccggtg
    15481 tcggtgctgt cgcggctgcg cgacttcctg gcggcgcgct gccgtggcaa cgagttgtgg
    15541 cgcggacacg aggccctgag cgacgaggtc ttcatcgagc ggcacggcaa ctggcgcggg
    15601 ccgtacgagg aagtgaccct gttcttctac gtcgatgaat acatcaagga cgctccgaag
    15661 gacctgctgg cggtgcttgg cgcgaccgcc gactggctgg agcgcagcct gaagaaggaa
    15721 tcgacgctcg tcgagaagaa catcgatgcg ctggccgggc tgctgcagct gaaccccgcc
    15781 gagcgcgcgc tgctgctcta cggcacgctg gcgcgttatc agcgcgacct gcgcggactg
    15841 ctggtcgagt tcaaggtgtc gagcgcccag gaggcccatg cggccatcgc cagcgtggcc
    15901 ggcgtcaacg aggccgatgt cgccgatgca ttgcgcgcgg gctcgcggct cgaacgcatc
    15961 ggcatggtcg agaacctgat ctccgaacac aacatcaccg atctcgccga cctgatgaag
    16021 gtcagcgagc aactgcctcc ggtgctgatg cggaagtacg agggaccgtc ggatctgatg
    16081 gcggttttca cgcgcccggt gcgtcgcagc gagctgctgc cctccgactt ccattacgtc
    16141 agcgaggacc gcgacgtgct gacggcgttg ctgagtcatg ccgtcgggcg ccgggagcac
    16201 ggggtcaacg tgctgctcta tggcccgccg ggcaccggca agaccgagct ggccaaggtc
    16261 gctgcgcagg cggcggggct ggagttgtac gaagtcgagt acgccgatcg tgatggcaac
    16321 agcctgaacg gccgggaccg ctaccgctcg ctgcagatca gccaggtgtt cctgaaggcc
    16381 agcgccagcg tggcgctgct gttcgatgag gtcgaggacg tgtttccgcc catttccacc
    16441 gacgccgcgc agctgatggc gcggatcgac accggtgatg gcgcgccgag cggcagcgtc
    16501 agcggcaagg cctgggtcaa ccagatcctg gagaccaacc cggtgccggt gatctgggtg
    16561 accaaccgca tcgagcagat cgatccggca ttccggcgcc gcttccagta ccacctcgag
    16621 ctgaagagcc cgccgcccgg agcgcgcgag gcgcttgtca cgcgtgccct ggcgggcgtg
    16681 cctgtctctg caggcttcgt ggcccgcctg gccgaacgac gtggtctgac accggcgcag
    16741 gtgcgtacgg ccgtcaagtt cgcccggctg gcgaccccgg ccgacgcact gctggacgca
    16801 gtctcggccg agcccggtac ccacagcgtg gccttcgagg cgctgatcga gcgacagatc
    16861 ggcaacgccg acaaggcgct cggtgcagac gtgcaggggc gtgcgccgcg cagcgtggtc
    16921 acctcctacg accttggcct ggtgaacacg gagtcgcgct tcgaggtgcc gaagatcgtc
    16981 gaggcgctgc gtcgcaaggc ctgcggcacg ctgtgcttcc acggcccgcc cggcaccggc
    17041 aagaccgcgt tggcagagca catcgcgcag cagatgcagc gcccgctgct ggtccggcag
    17101 gcgtcggaca tcatgagcaa gttcgttggc gagaccgaac agaacatggc gcgcatgttc
    17161 gaggaggcgc agctcgagca ggcggtgctg ctcctcgacg aggccgacag cttcctgcgc
    17221 agccggcgga tggcggagcg caactacgag atcagcgaag tcaacgagat gctgcagggc
    17281 atggagcgct ttgccggcgt cttcatctgc accaccaacc tgttcggcga gctcgatgag
    17341 gcggcactgc gccgcttcac attcaaggtt cgcttcaagc cgctgactgc cgagcagcgc
    17401 gagcgaatgt tcgtcgccga ggcgctcggt ggtgaccccg cgcggctcac cgccgagcag
    17461 caggcgaggc tgctcgagct cgaatcgctc gcgcccggcg actacgcttc ggtcaagcgc
    17521 cagatcgacg tgctgggaga agccttcgag cccgacgagt tcctgtcgca actggaagcg
    17581 gagcaccgcg tcaagcccga ggtgcgtcag cagcgccaca tcgggttcat gcactgactt
    17641 tttctatgct gcggggcggc tgcgccctca agtgacgtcc gacgggcgcc gatagcaccg
    17701 catgatcgaa ctgttcgtgc agtcgctgtt cgcgctggac tggaagctgg cattcctggc
    17761 ggcattcgtc tgcctggacc tggcccggcg tctcgagtcg aagctcgacc tcgcgggttg
    17821 gggctggctg ctgggcgcgt cgctggcact ggctaccggc ctctggtcgg cccaggtgat
    17881 cggcatcgag cgactccagc ttccgtatgc catcgaatac cgcccgggac cggcctttgg
    17941 cgtctggctc ggcgccggag ccatcagcgc gctgggtctg gccttggcgt gccggcggtc
    18001 gtccggcctg tctgtcacgc tgatcggtgc cttgctgctt gccgcgggca tcgttgccat
    18061 tcagatgtgc ttcgtcgagg cactgtggct gcagcccggc ccggcctggc ggctcccggg
    18121 cctgggcatg gcgctggtgg cggcgagcgc gggctgcgcg gcggcgctgt ggctcttcgc
    18181 agagattcgc gttcgggcca gggagggggc gctggcctgg caggccgcct ctgcactcgt
    18241 cctgggcctc tcgatcgtca tttcccaggg tgtcgtgatg ggcgcggcgg ggctcgagag
    18301 tccgcacgtg tcggtggtca cgcaggactt cctttcggaa gacaccatgg ctgttctggt
    18361 gaaggtggtt tcggccgcgc tgctgtcgat gttgctggtg ctgtcgcaga tcgaggcgca
    18421 cctgcgtgcg tccctgcaga gagcgaaggg cgagctgcgc cagcaatcgc tgcgcgatcc
    18481 attgaccgag ctgcccaacc gccagatctt cgagggcacg ctggcgcagg ccgcgcgcca
    18541 ggcggatgcc gaccagggac agctggcgct gatgctgatc aacctcgatg gattcaagcc
    18601 gatcaacgag tctctgggtc atcgtgtggg cgatcgtgtc ctgcgcgaga tcgccgcgcg
    18661 actgcgcagt ctggcgcggg tgcacatggt cgcgcgattg ggcgccgacg aattcctgct
    18721 gctgatgcca gacaacccga ccaccgaaca ggtgtccgat ctggcggcgc gggtgctggg
    18781 ctcgctgagc ctgccctgcc cgatcgatgg acgcgaaacc acggtgaccg cgtcgatcgg
    18841 catcgtgatg taccccgagc atggcgcggt ctcgaccctg gtggctcatg cagacgcggc
    18901 gatgcgattt gccaagtcca gcgggggtgc gacctactgc ttcttcgagg cgcgcatggt
    18961 gagcggcctg cgcgagcaga ccgagttgct gcgcgacctg cgccgggcac tggcccaggg
    19021 cgagctcgag ctgttctatc agcccaagat ccacgcgccg acgggcgaga tcaccggcgc
    19081 cgaggcgctg atgcgctggc agcatccgcg gctcggcatg atcagcccgg gcgtcttcat
    19141 tccgatcgcc gagcgcttcg ggttgatcgg ctcgctcggc aactggctga tcgacgaggt
    19201 gtgccggcag atccgcgtct ggcgggacgg cgggctgcgc atgagagtcg cgatcaacct
    19261 gtcggtgcac cagctgcgcc agcccgatct ggtcgagcgc atcgcacgcg cattgcgcga
    19321 gcacgacgtc aatccgggcc tgctgacctg cgagatcacc gagtcggtgg cgatgcagga
    19381 cgccgagggc acgatcaagt tcttcgggca gctggcgcag ctgggtgtca gcatctcgat
    19441 cgacgatttc ggaacgggtc attcgagcct ggcctacctg cgcaagctgc cggccagcga
    19501 actgaagatc gaccgcagct tcgtgctcga cctggagacc agcgaggacg cccgcaaggt
    19561 ggcctcggcg gtggtcaacc tcgccaaggc gctgaacctg aaggtggtgg ccgaaggggt
    19621 cgagaccgag ggccagaacc gcatcctgcg cgagttcggg tgcgaccagc tgcagggcta
    19681 cctgttcgcc aagccgatgt ccgcgaagtc gctcgccttg tgggcgatgg atgacgtcgg
    19741 gccgcgatcg atcagtttcc gttcgtccct gttcctcgaa acccagcctg ccgaactggt
    19801 ctgagggccg cggtcgcgtc gattcgcagg gcggttccta gaatcggggc atgaaaaccc
    19861 agccctacac ccgcggcgcc cagttgcccg acctgctgcg acagcgcatc gtcatcctcg
    19921 acggcgccat gggcacgatg atccagcgct acaagcttgg cgaagccgat taccgcggcg
    19981 agcgcttcaa ggaccatccc agggatctga agggcaacaa cgagttgctg cttttcacgc
    20041 ggccggacgt gatctccgag atccacgacc agtacctcgc ggccggctcg gacatcatcg
    20101 agaccaacac cttcggcgcc acgcgcatcg cccaggacga ctacggcctc gcgtcggtgg
    20161 cgcgcgagat gaacgtggtg ggcgccaggc tggcgcgcga ggcctgcgat cgctacagca
    20221 ccgccgacaa gccccgcttc gccgccggcg cgctgggtcc gacgccgcgc accgcgagca
    20281 tcagccccga tgtcaacgac ccggccgcgc gcaacgtcag cttcgacgag ctgaaggact
    20341 cgtacctcga gcaggcggcg ggcctgctgg aaggcggctg cgacctgttc ctcgtcgaga
    20401 ccatcttcga caccctgaat gccaaggccg ccatcttcgc gctggacgag ctgatggagt
    20461 cgaccggtga acggctgccg gtcatcatct ccggcacggt caccgacgcg tcggggcgca
    20521 tcctgtcggg acagacggtc accgcgttct ggcacagcgt gcgccacgca cggccgctgg
    20581 cgatcggact gaactgcgcg ctcggtgcga cgctgatgcg tccctacatc gaggagctgt
    20641 ccaaggtcgc tggagacacg ttcatcagct gctacccgaa cgccggtctg ccgaatccga
    20701 tgagcgacac cggtttcgat gaaacgccgg agatcaccgg ccggctggtc gaggagttcg
    20761 cgaagagcgg cttcctgaac atcgccggtg gctgctgcgg caccacgcct gcgcacatcg
    20821 cgcagatcgc gcagcgtgtc ggtgcttatc gcccgcgctg cctgcacgag cggccgttca
    20881 gcagcctgct ggagtcgacc gccgcggctt gaagccggcc agcgtgccga cgcgcgtcac
    20941 atgagctcga tcaggctctc gacgctcagg cgcaccttga cttcgtgcgg tgcggcgctg
    21001 ccggtgatct cgtaggcctt gtgccgcgtg tcgcgcacgg ctggtttcga catcggtatg
    21061 ccgttgatgc cgaccacgca cgccaccacc gggatgctgg cgcgcaggcc gcgcttgagg
    21121 acgatggcga cttcacctcc ggccaggcgc acgaagcagc cgggtggata gatgcctgca
    21181 gccttgatga tggcggcccc ggcctcgtca ggctgctggt tctcgtcgag gtaggcggcc
    21241 ttggccgctg cggtggccgt catggccttg cgcgtcttgc gggggctcag gcgtgcggca
    21301 aaaacgtcgg cgcgcttgat cagccgtgcg atctgcaacc cgggctccat ggcctggagg
    21361 gggcccggcg ggctggtgtg gtggtgttcg acggcctgca gccagagttc gtctgcgacg
    21421 cccagctcgc gaagctgtgc gacaccgcgt gccgcgtggc tatcgatcag ctcgcgctgc
    21481 tgtggcgttg tcgccgagtc ctgcaccgcc agctggtcct gcaggttggt catcgcgatg
    21541 ttcatggtca gcgaagcccg gcgtagggct gccttccact ccctgggcca gccgttcagg
    21601 tggcgggcgg ccagttcgac gatcgtggtg acgagcagcg catgacgaac gctgtacttc
    21661 tggctgtcgg tcgtggcgct gtagatcagc gacagcaggg ccgcgtccgc accacgcgag
    21721 atgagcgata gcagatcctc gtcgatgcga tcgatgcggt cgatgaaatc ttctgccgac
    21781 ggttcgcgca acgcggcggc catcttcatg acgaggccgt cccaggtgag actggtgtcg
    21841 ccgcgccggg cgcgttcggg cacgccgaag ttgtccatca cgctgtcggg gtcgacctcg
    21901 gcctcggcct gggcaatgcg gccgatccgt gcgttgtccc tcaccatcga atccatcttg
    21961 cccgccaatg ccttcttgaa ggcgtcggcg tcgctgatgt cgatgtagac accccgttcc
    22021 gtgagttgtt tgcgcacact ttcgctggga accacgcccc cgcgaggaac caacatcaag
    22081 cctgcagcgt cgcgcagggc gaacggaacc ggctggttga tgcgaatggc actggcggag
    22141 atgggtacaa cggccatgac gatggggcag gaatgtggaa cggacagctt ccgtgcagtg
    22201 gatatcggag tgccgtgacc tgacttgagt tgttgcacgg cacaaaacat gcgcgcctac
    22261 aatcgcttct ctttcgagga gcgctgcgac aacccctgaa aacgccacgg gcggcgggtt
    22321 gccaggctcg aaagtgcgag gtttcaacgg cgctcccccg accctcacgg gccgcggtgg
    22381 gctgcgcggc atgtgacatt tgacactccg ccgaacgcca agactgccgc catgctcccc
    22441 gtcccgtcca cacctccctc catcgtcgag ccgatgaagc tgtccgggct cgaaccggtg
    22501 ctgatcggca acggctcgct gttcgtcaac atcggcgagc gcaccaatgt caccgggtcg
    22561 aaggccttcg cccgcatgat cctgaacggc gatttcgagc aggcgctgtc cgtggcgcgc
    22621 cagcaggtcg agaacggcgc ccagatcatc gacatcaaca tggacgaggc catgctggac
    22681 agccaggcgg cgatggtgcg cttcctgaac ctgatcgcca gcgagcccga gatcgcgcgc
    22741 gtgccgatca tgatcgacag ctcgaagtgg agcgtgatcg aagccggcct gaagtgcatc
    22801 cagggcaagg gcatcgtgaa ctcgatctcg ctcaaggagg gcgtcgagca gttcaggcat
    22861 caggccaagc tggtgcgccg ctacggtgcg gctgcggtgg tgatggcctt cgacgagaag
    22921 ggacaggccg acacctacca gcgcaagacc gagatctgcg agcgcgccta tcgcatcctg
    22981 gtcgacgagg tcggcttccc gcccgaggac atcatcttcg acccgaacat cttcgcgatc
    23041 gccaccggca tcgaggagca cgacaactac gcggtcgact tcatcaacgc cacccggtgg
    23101 atcaagcagc acctgccggg cgccaaggtc agtggcggcg tcagcaacgt cagcttcagc
    23161 ttccgcggca acgagccggt gcgcgaggcc atccacaccg tgttcctgta ccacgcgatc
    23221 cgcgccgggc tggacatggg catcgtcaac gccggcatgg tgggcgtgta cgacgacctt
    23281 gcgcccgagc tgcgcgagcg ggtggaagac gtggtgctga accgccggcc cgacagcacc
    23341 gagcggctgc tggagattgc cgagaacgtg aagggcgcgg ccaaggacga cagccagcgc
    23401 ctggcctggc gcagcggcac ggtcgacgag cgcctgagcc atgccctggt gcacggcatc
    23461 accgacttca tcaccgaaga caccgaggcc tgctggcagc gcattgccgc cactggcggc
    23521 cggccgctgc atgtgatcga aggcccgctg atggccggca tgaacgtggt gggcgacctg
    23581 ttcggccagg gcaagatgtt cctgccgcag gtggtcaaga gcgcccgcgt gatgaagcag
    23641 gcggtcgcgc acttgctgcc ctacatcgaa gccgagaagc tcgcgctggt cgaagctggt
    23701 ggagaggcga aggccaaggg caagatcatc atcgccacgg tcaagggcga cgtgcacgac
    23761 atcggcaaga acatcgtgac ggtggtcctg cagtgcaaca acttcgaggt cgtgaacatg
    23821 ggcgtgatgg tgccgtgcca cgacatcctg gcgcgtgcca aggtcgaagg cgccgacatc
    23881 atcggcctgt cgggcctgat cacgcccagc ctcgaggaga tgcagcatgt cgccgccgag
    23941 atgcagcgcg acgactactt ccgcgtcaag aagatcccgc tgctcatcgg cggtgccacc
    24001 tgctcgcggg tgcacaccgc ggtgaagatc tcgccgcact acgaaggccc ggtggtctac
    24061 gtgcccgatg ccagtcgcag cgtcggcgtg tgcagcgacc tgctcagcga cgagaaggct
    24121 gctgcctaca tcgccgagct gaagtccgac tacgacaagg tccgcgagca gcacgccaac
    24181 aggaaggcga cgccgctggt catgctggcc gaggcgcgcg ccaacaaggt gcccatcgat
    24241 tgggtgaact acgctgcgcc gaagccgaag ttcaccgggc gccgcgtgtt ccgcaactac
    24301 gacctggccg aactggccca gttcatcgac tgggccccgt tcttccagac ctgggacctg
    24361 gccggcgcct atccggcgat cctgaccgac gacatcgtcg gcgaatcggc gcgccgcgtg
    24421 ctgagcgacg ggcagcgcct gctcaagcgc ctgatcgacg ggcgctggct gactgccaat
    24481 ggcgtgatcg gcctgtatcc agccaacacg gtgggcgacg acgacatcga gatctacacc
    24541 gacgagaccc gcagcaaggt gctgttcacc tggcgcggcc ttcggatgca gtcggtgcgg
    24601 ccggtcgtcg atggcgtgat gcggccgaac cgctgccttg ccgactacgt cgcgccgaaa
    24661 gattcaggcg tggctgatca catcggattg ttcgcagtga cgtcgggcat cggcgctgaa
    24721 aagaaggaaa agcagtttct cgacgaccac gacgactaca gctcgatcat gctgaaagcg
    24781 ctggccgatc ggctggccga ggccttcgcc gagcgcatgc atcagcgtgt gcgcaccgag
    24841 ttctggggtt attcggccga agagtccctg acgcctgccg agctgatcgc cgagaagtac
    24901 cgcggcatcc gtcccgctcc gggctacccg gcctgccccg accacacggt caagggtgcg
    24961 atgttcgagg cgttgaactg cgcggacatc ggcatggcgc tgaccgagag ctgggcgatg
    25021 tcgccggcgg ccagtgtcag tggcttctac attgcccacc cggacgcgac ctatttcaac
    25081 gtcggcaagg tgggcgaaga ccagatcgag gattgggcac ggcgcacggc gcaggatgcc
    25141 gcacaggtgc ggcggtcgct cgcggcgctg ctttaagcca ccgcagccgg cgcgcggcgg
    25201 ctagcgcgct gccagcacgc gcctgtactg gatcgcttcg gccaggtgag caacctggat
    25261 cgactcgctg gcggccaggt cggccacggt gcgtgccatg cgcagcaccc ggtggtgact
    25321 gcgcgccgac catgccagtc gtgaagccgc cgctgtcagg aagcgagatg ccgcgtcgtc
    25381 gagccggcag tggatgtcca gggcctcgcc ccacagcgtc gagttcaggt tcccctgccg
    25441 gtcgtgctgc agctgctgcg cgacagccac gcgcgcggcc accacggccg tcggttcacc
    25501 gtcgggcgca gcggccagcg cgtcggccga cagcgcgggc acctcgacct gcagatcgat
    25561 gcggtcgagc agcgggccac tgatgcgacc ctggtagcgg ctgacggcat ccggcgagca
    25621 tcggcaggat cgcagcgtgc tgccgtggtg gccgcagggg catgggttca tcgcggccag
    25681 cagctggaat cgcgctggga actccgcctg tcttgccgca cgcgagatga cgattcgtcc
    25741 ggtttcgagc ggctcgcgca gggcctccag cgccatgcgc gggaactcgg gcagttcgtc
    25801 gaggaacagc acgccttcgt gtgccagcga gatttccccg gggcgaggcg gcgagccgcc
    25861 tccaaccagt gccaccgacg aggcgctgtg atgcgggctg cgatagggcc tgcgcgccca
    25921 ttgactggcg tcgaatgcgc tgccgttcaa gctgatcacc gcagctgatt ccagcgcctg
    25981 agcctcggac agcgccggca gcagcccgca gaaccggtgc gccagcatcg acttccccgt
    26041 gcctggcggg ccggtcatca gcacgctgtg tccgccggcg gcagccacct ccagcgcacg
    26101 cttggcggcg gcctgacctt tcacatcgcg caggtcgatt gctgcagctt gcggctgctc
    26161 accccgggtc gcgagcagcg agcaggccag gggcagctcg acccgcagtt cagcatcaac
    26221 cggcaacagc gagcgcacga catccagcag gtgcatggcg ccttggatcc gcagcccagg
    26281 caccaacgca gcttcctgag cactcgtttg aggcagaacc agcgcccgct ccgagaaccc
    26341 gcgcttgagc gcgagggcca tggcgatggc acctcggatg ggtctcagat caccggcaag
    26401 agacagttca ccggcgtatt ccgcctgggc cagtcgctgt gcgtcgatct ggccgctggc
    26461 ggccaggatg ccgagcgcga tgggcaggtc gaaacgcccc gactccttgg gcagatccgc
    26521 gggagccaga ttcaccgtga tgcgcttgtt gtgcggaaac tccagaccgc tgttctgcaa
    26581 tgcggcgcga acgcgttcgc gagcttcctt cacctcggta tcggccagcc ccaccagcgt
    26641 gaagctgggc aaaccgttgg caaggtgaac ttccaccgtg acgggcggag cgatcaagcc
    26701 atcgagggcg cggctgtgga cgagtgcgag ggccatgatc gagcgatcgt ggcatttgca
    26761 ggcgtgtggc gactcccccg gaggagggtt cagcccgaaa cggcaccatg aaggtgcgca
    26821 aatcgcgtaa ttgcccccaa acagggcgac ccggagctac gtctattggt gtttaccgct
    26881 tgtttctcat ggtgttgggc cgggcacggt ttctgcaggc cgtcagcagg catcgccctc
    26941 cctccactct caaaaagcga aacccatgaa caagactcaa attgccctcg cgaccgcact
    27001 gctggcctcg gtgtcgacac tcacccacgc agccgatgct gcacccgctc ctgctccgac
    27061 gccggattgg acgatcaccg gaaatgcggc gctgtccagc gactaccgca ctcgcgggat
    27121 ttcgcagaca gacaggcgtg cggcgttctc gggcggattc gatgtggcgc acagctccgg
    27181 cttctacatc ggcaactgga actcgaacat cgacagtgat ttctacaccg gtgccaacct
    27241 cgaactcgac ttctgggccg gctacaaggg gtctgtcgag ggcatcggct acgacatcgg
    27301 cgtcttctac tactactacc caggcagcga tcgccagggc gctgcaggta tcgacaacaa
    27361 ggagatctac gtcggcacca gctatggccc ggtcagcgcc aggctgtata tcccggtcga
    27421 cgacttcttc agcgcgcaac gacagaactt tggcaacggc aagaacgccg ctggttcgta
    27481 ctacctggac gttgcaggtt cctacgatct gggcaacagc ttcggcctga tcggtcactt
    27541 cggctatcag aagctcaagg gtgccgccaa gctgcaggag atcggcaaga gcaagttcga
    27601 gaacgacatc atcgactgga agctcggcgt cacatacacg ttcagcaatg gtttcgtggc
    27661 gacggtgacc tacatcgata ccgacatcaa gatcgcgggt ccttccggca agaagatcag
    27721 cgggcccacg ggcgtcttca gcatttccaa gacgttctga acgccatcaa cccttaaagg
    27781 agaacgccat gaagatggtg acagccatcg tcaagccctt caagcttgat gaagtccgtg
    27841 aggcactcag cgccattggc gtgcagggcg tgacggtgac cgaagtcaag ggttttggca
    27901 gacagaaggg ccacaccgag ctgtatcgcg gcgcggagta cgtggtggac ttccttccta
    27961 aagtcaagat cgaagccgcg gtcgacgaca gcatcgtcga ccgggtcatc gaggccatag
    28021 aaaacgccgc ccgcaccgga aagatcggtg acggcaagat cttcgtgtct tcgctggagc
    28081 aggtcgtccg catccgcact ggcgagacgg gcaaagacgc gctctgaagc ccaaccgaga
    28141 aagaccagcc ccatgaaaaa actaattgct actctcgccc tcggactcgc ggtcctgggc
    28201 ttctccgtcg cgactttcgc gcaagacggt gcagcgtccg ctcccgcggt ggcagaggcc
    28261 gcggtcatgg cgaccgaagc ggcatctgca gctgctgagg ctgtggcccc ggctcctgcc
    28321 gaagctgcgg cggccaccga ggcgccggca gccgcaccgc ctgtgccgaa caagggtgac
    28381 accgcctgga tgatggtctc gacgatcatc gtcatcatga tggcgatacc cggccttgcg
    28441 ctgttctatg gcggcctggt ccgcagcaag aacatgctgt cggtgctgat gcaggtgatg
    28501 gtgacgttct cgatgatcgt cgtgctgtgg ttcgtctacg gctacagcct ggcgttcacc
    28561 gaaggcaacg ccttcttcgg cgggctcgac aggctgatga tgaagggcgt gtgggacaac
    28621 gcagcgggga cctttaccaa tgcggcgacg ttcagcaagg gtgtctacat tccggagatc
    28681 gtcttcgcgg tgttccaggc atcgttcgct gccatcacct gcgcgctgat cgtcggtgcc
    28741 ttcgccgagc gtgcgaagtt ctcggcggtg ctgttgttca tggccctctg gttcaccttc
    28801 agctacgcgc cgatggccca catggtctgg ttctggatgg gtccggatgc ctattccagc
    28861 aaggaagtcg tcgatgagat gacgtcgaag gccggctaca tctggcaatc cggcgcgctc
    28921 gactttgctg gcggcaccgt ggtgcacatc aatgcagccg tcgccggtct ggtcggtgcg
    28981 tacatgttcg gcaagcgcgt cggctatggc aaggaggcgt tcacgcctca ctcgctgacg
    29041 ctgacgatgg tcggtgcttc gctgctgtgg gtgggctggt tcggcttcaa cgccggctcc
    29101 gctctcgagg ccaacggctt tgcggccctg gcattcatca acaccttcgg cgccactgca
    29161 gctgcagtgc tggcttggtg tgcaggcgag gccctgatga agggcaaggc gtcgatgctg
    29221 ggtgcagctt ccggagcggt ggcgggtctg gtcgccatca cgccggcttg cggcaacgtg
    29281 ggtatcggcg gtgctttgat catcggtgtg atcgccggct tcgcatgcct gtggggtgtc
    29341 agcggcctga agaagatcct cggtgccgac gattcgctcg acgtgttcgg tgttcacggc
    29401 gtgggcggca tcctcggtgc cttgctgacg ggcgtgttca atgcaccgag tctgggcggc
    29461 ccgggctacg tggccgactg ggtcaccgtc gcgatggtga cgccagctga ctattcgatc
    29521 gctgcccagg tctggatcca ggccaaggct gtgttgatca ccatcgtctg gtcgggcgtg
    29581 gtgtcggtcg tcgccttcaa ggtcgtcgat ctgctgatcg gcctgcgtgt gccggaagac
    29641 gaggagcgcg aaggcctcga catcacgtca cacggcgaga cggcctacca ccagtgagcc
    29701 ccctcggttt cggagcaccc gcctgagctt ccgggtgcca ccgaaccggg agcaagagcc
    29761 gccgcaaggc ggctcttgtc ttttgtgtgt ccgtttgccc tccgtcgggt cgatcgctgc
    29821 agtctcgcag ctgaaaatcg ccaagccgag tatcgttatc cgtcaatcgc aaaacgggct
    29881 cttgccatgg ttcctcatct catcactgca ctgaccggcc cgatcaacga actggaacag
    29941 cggatcctgg agtcgctgcc cgccatcgag cgatggttcc ggctcgaatg gatggaacac
    30001 acgccgccgt tctacacgtc ggtggatgtg cgaaacgccg gcttcaagct ggcgccggtg
    30061 gacaccaacc tgtttcccgg cgggttcaac aacctgaccg ccgagatgct ccccctggcg
    30121 gtgcaggcgg cgatggccgc gatcgagaag atctgccccg aggcgaaaaa cctgctgctg
    30181 atcccggaaa agcacacccg caacaccttc tatctggcca atgtcgcgcg gctgatgcag
    30241 atcttccaca tggccggcat gaacgtccgc ctgggcacgc tggacgacac catcaccgtc
    30301 ccgacgccga tcgagctgtc cgacggtacc cagttgacgc tggagcccct gattcggaac
    30361 cggggtcgcc tggggctgaa ggatttcgac ccctgcacga tcctgctcaa caacgatctg
    30421 tcggccggca tccccaaggt gctcgagaac ctgcacgagc agtacctgtt gccgccgctg
    30481 catgccggct gggccattcg ccgcaagacg aaccatttcg ccgcctacga ggaagtggcc
    30541 aagaagttcg cgaagctgct gggcatggac ccgtggttga tcaatccgat gttcgcgcgt
    30601 tgcggcgagg tgaatttcag cgacgggacc ggctccgaat gcctgatgag caatgccgag
    30661 gcgctgctgg ccaaggtcaa gcgcaagtac aaggaatacg gtatccagga gaagccgttc
    30721 atcatcgtca aggccgatgc cggcacgtac ggcatgggca tcatgaccgt gcgcgacccg
    30781 aaggaactgg ctgaactgaa ccggcgctcg cgcaacaaga tgagcgtcat caaggacggg
    30841 caggaggtca gcgaggtgat cctccaggag ggtgtgccga cctacgagcg cgtcaacgac
    30901 gccgtcgccg agccggtggt ctacatgatc gaccgctatg tggtgggcgg cttctacaga
    30961 gtcaatgccg agcgtgggat cgacgagaac ctcaattcac ccggcgcgag cttcgtgccg
    31021 ctcgcctttg cagagtcgaa ccagctgccc cgaccgggcg tcaagccggg cgccagcgca
    31081 ccaaaccgct tttacatgta cggcgtgatc gcgcgtctgg cgatggtggc ggcgagctac
    31141 gagctcgaag ccaccgatcc cgacgctgaa atctacgagt gaggcgagcg gccacagttg
    31201 ctgcaccgca acattccttg gtgtaagacc cgcttttgcc gggtgcgcgc cccggggcaa
    31261 aggtatcgcg gggagcacaa tccattgtcg tttcgtacac gctctggccg gcttgacgcc
    31321 ggttcatctt gactccccat cgccccgctt ccagccttgc agccctgacc ctgggcgcca
    31381 tcggcgtcgt ttacggagac atcggcacca gcccgctgta cgcgctcaag gaggtcttcg
    31441 ctcacggcca cgtgccgctg actccagaca acatctacgg tgtgctgtcg ctcgtgttct
    31501 ggacgctgag catcgtggtg tccgtcaagt acgtgatgct gatcctgaga gccgataaca
    31561 acggcgaggg tggcttgatc gcgatgttgg cgcttgcttc gcaggcggtg agggagcagc
    31621 ccgtgctgcg ccggcgcctg ctgctgctgg gcattttcgg cactgcgatc ttctttggcg
    31681 atggcgtcat cactccggcg atctcggtgc tgtcggcggt cgaggggctg gaggtcgccg
    31741 cccccgggtt gcatcgctat gtggtgcctc tgacgcttgc ggtgctgacg ctgttgttca
    31801 tctttcaacg gttcggcacg ggcagcgtcg ggcgcctctt cgggccgatc accgtgctgt
    31861 ggttcgctgt gctggcggcg ctcggcgccg tgcacatcgc agagaacccg gcgatcctgt
    31921 gggcgctgag cccgcaccat gcactgggtt tcatggtgtc acagccggca atcgccttca
    31981 tcgccctcgg ttcggtggtg ctgtgcgtca cgggcgcaga ggccctctat gccgacatgg
    32041 ggcacttcgg caagcgaccg atccggctgg cctggttcag cctggtgatg ccggcgctgg
    32101 tcatcaacta cttcggccag ggcgcgatgc tgctgctgca tccggagaac gtcaggaacc
    32161 cgttctacga gatggcgccg acctgggcgc tttacccgct gatcggcctg gcgacctgcg
    32221 ccaccgtgat tgcatcgcag gcgctgatca cggcggcgtt ctcggtgacc aagcaggcca
    32281 tccagctc
//
LOCUS       DAOORV010000023        31236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1672_length_31236_cov_8.539656, whole genome shotgun sequence.
ACCESSION   DAOORV010000023 DAOORV010000000
VERSION     DAOORV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 31236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_31236_cov_8.539656"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKV56_04980"
     CDS             <1..637
                     /locus_tag="PKV56_04980"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOX67200.1"
                     /translation="TRSDIEVFLPPIGGQTLYILGDPRSLADPSITLTARVHDECNGS
                     DVFGSDICTCRPYLTHAIEECIRGAQQGGVGLIAYSRKEGRALGEVTKFLVYNARKRQ
                     LGGDSADKYFLRTECVAGVQDMRFQELMPDVLHWLGIRKIHRLVSMSNDKYDAITGSG
                     IEVGERIKIPDELVPADARVEIDAKIAAGYFTDGAVPSAADLAATKGRDLE"
     gene            630..1904
                     /locus_tag="PKV56_04985"
     CDS             630..1904
                     /locus_tag="PKV56_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HOX67201.1"
                     /translation="MSEIVLASPHGRDAVSVLRDPATIRRRCAAVTQAISEGRSANFK
                     LDRSRLDEVAARVERVTRAAYPELRIPFHSRWRHFASGGIDRKASMDAQMADRSVADQ
                     ARARFDLTVVSVLLDAGAGPQWRYVEDGTGQTFSRSEGLGIASWHAFQQGLFSSAPGD
                     DPLRADAAALQRLDASTLRRSFQVTPGNPLVGLEGRVALLNRLGAALAADQGMAGRPS
                     ALFDRLTQSASVSDGTAEVDAGQVLRAVLDAFSSIWLTGSMLQGVPLGDVWPHRWAGG
                     ESKSEGGHGTTDGWVPFHKLSQWLSYSLLEPLIWSGAHVTGLDALTGLPEYRNGGLLL
                     DGGVIVPRDPRNLARRWTPADEFIVEWRALTVSLIDELAPLVRARLGKTAEEMPLACI
                     LEGGTWAAGREIARELRDGSPPLSIDSDGTVF"
     gene            1925..2557
                     /gene="upp"
                     /locus_tag="PKV56_04990"
     CDS             1925..2557
                     /gene="upp"
                     /locus_tag="PKV56_04990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOX67202.1"
                     /translation="MTAPVHHVTHPLIQHKLTLMRQKDCSTNSFRRLMSEISMLMAYE
                     VTRDMPTQLIDIETPLEAMKSPVIDGKKTVFVSIMRAGGGFLDGMLTVVPGARIGHIG
                     LYRDPKTLVAVEYYFKMPSDMHERDAIVLDPMLATGNSAVAAVDRLKETQPRSIRFVC
                     LLAAPEGLKAFQSAHPDVPVYTAAIDRELNDHGYILPGLGDAGDRLFGTK"
     gene            2560..3831
                     /locus_tag="PKV56_04995"
     CDS             2560..3831
                     /locus_tag="PKV56_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX67203.1"
                     /translation="MLIRNATLPDGSTGVDVLLRDGRIAGIGTQLACPENAREVDARG
                     HLLAPSFVDAHFHMDATLSYGLPRVNASGTLLEGIALWGELKPQLTQDALVARALQYC
                     DWAVAKGLLAIRTHVDVCDDRLLAVEALLQVKRQVAPYLDLQLVAFPQDGLLRSPNAL
                     ANLKRALDLGVDVVGGIPHFERTMAEGTESIRILCELAAERGLRVDMHCDESDDPLSR
                     HVETLAFHTQRLGMHGRVTGSHLTSMHSMDNYYVSKLIPLMAEAQVNVVANPLINITL
                     QGRHDSYPKRRGMTRVPELMAAGLNVAFGHDCAMDPWYPLGSADMLEVAHMGLHVAQM
                     TSPKQMEACFDAVTVCGAKAMGLDRHGITVGCDADLVLLQARSVIEALRLRPNRLLVL
                     RKGKMVACSAPAMSQLDLPDRPASVDWTLRR"
     gene            3969..4589
                     /locus_tag="PKV56_05000"
     CDS             3969..4589
                     /locus_tag="PKV56_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOX67204.1"
                     /translation="MKLIGSLTSPYVRKVRIVLAEKKLDYHLEVEDVWGNDRILASNP
                     LGKVPCLVMEGGEAVFDSRVIVEYVDTLSPVGRLIPERGRERAEVRTWEALADGVLDA
                     AISARLEQTWAGRSEAERSSAWIGRQMDKVNHSVAAMSKGLADKPWCCNGNHLTLADI
                     AVGCALSYLDFRFPQIDWRSPHPNLVRLSDKLCGRPSFIDTQPPAA"
     gene            complement(4623..6314)
                     /locus_tag="PKV56_05005"
     CDS             complement(4623..6314)
                     /locus_tag="PKV56_05005"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOX67205.1"
                     /translation="MQTWLEKLNHHFPLRYSAWFLCAVGLVVSLSLSLLMRMPSGWDW
                     VLPSIFAGLVLLGVRDTRQGTHSVLRNYPVIGHMRYLFEFIRPEIRQYFIEGDNEAAP
                     FSRQQRSLVYQRAKGDQDKRPFGTQKDVSAQGYEWINHSMHPTHLPTHDFRVVIGGGH
                     GDPAHSQGAHGTPCTQPYTASVFNISAMSFGALSANAILALNAGAKRGGFAHDTGEGS
                     ISRHHRGPDPDGGGDLIWEIGSGYFGCRHPDGSFNPDLFATNARLAQVKMIEIKLSQG
                     AKPGHGGVLPGPKVTPEIAEARGVPPWVDCVSPASHSAFSTPIEMMQFVDRLRALSGG
                     KPVGFKLCIGHPWEWFAIAKAMLATGITPDFIVVDGGEGGTGAAPLEFTDHVGAPLQE
                     GLLLVHNTLVGLNLRHRVKIGCAGKVVSAFDIARNMALGADWCNSARGFMFALGCIQA
                     QACHTGDCPTGVSTQDPHRQQALVVPTKADRVFNFHQSTLKALKELVQAAGLDHPGAI
                     TAAHIVRRTNDHEVKLLSNLLPFVKPGSLLTGDLTHSVYRLYWPMAQAESFGVAM"
     gene            6644..7852
                     /locus_tag="PKV56_05010"
     CDS             6644..7852
                     /locus_tag="PKV56_05010"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOX67206.1"
                     /translation="MSSLGHVRPRSMSFAEPLTLRCGAVLRDYTLVYETYGTLNAERS
                     NAVLVCHALNASHHVAGTYEGDPKSTGWWDNLIGPGRPLDTDRFFVIGVNNPGSCFGS
                     TGPTHINPATQQPWGVDFPVMTVEDWVDAQARLCDALGIEQLAAVIGGSLGGMQALCW
                     SMRYPSRLRHCIAIATAPNLSAQNIAFNEVARRAIVTDPDFHDGHYLAHGVVPKRGLR
                     VARMIGHITYLSDDTMDAKFGRTLRAAIGAEVVQRSGSPRYTTLDIEFEIESYLRHQG
                     DKFSEYFDANSYLLITRVLDYFDPAREHDGDLSAAFAAATARFQLVSFTTDWRFSPAR
                     SREIVKALLDNRLDVSYAEIAAPHGHDAFLLDDSRYHGVLRARFERIAREIDAGSHAD
                     LARPGLGIAT"
     gene            7849..8433
                     /gene="metW"
                     /locus_tag="PKV56_05015"
     CDS             7849..8433
                     /gene="metW"
                     /locus_tag="PKV56_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOX67207.1"
                     /translation="MSAGNDLDLIAGLVPTGSRVLDLGCGSGETLAYLRDRRQCTGYG
                     IEIADANVLACEQRGVNVIQLNLEEGLAIFDDQSFDVVLQIDTLQHLRNTERMLAETA
                     RVGRIGIVTFPNFAHWPNRLSVIRGRMPVTKALPYEWYDTPNIRVSTYADFEVLARRC
                     GLRILDAFGIQLGAPVRLCPNLLASVAVFKFERA"
     gene            complement(8414..9055)
                     /locus_tag="PKV56_05020"
     CDS             complement(8414..9055)
                     /locus_tag="PKV56_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOX67208.1"
                     /translation="MARRTKDDALATRDRILDAAELVFEQRGVSRCSLQEIAQAAGVT
                     RGAIYWHFENKADLVNAMFKRVTLPLDEAINRSADPDTADSVLEVRNSLLNALRKTVA
                     DPQLQRVFVIKMQKVEYVDELLVVRDRRVNNRAGLIRNVEVGLERAMATGRLNRRTPA
                     LATAIGLYALTSGLIENWLLDPSAFDLVQVGEQALDAYLCGLTLPASTTPART"
     gene            9190..10437
                     /locus_tag="PKV56_05025"
     CDS             9190..10437
                     /locus_tag="PKV56_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX67209.1"
                     /translation="MSVHKRIGFRSSMVLAVVGATWLIASLSGCGGKPGETSPASAAS
                     APPPAVGVITVTPQPVGMASELPGRLEATRIAQVRARAAGILQKRLFREGSDVKAGQL
                     LFQIDAAPYRAQLQSAQAAQARAEANREQAGAQAERYKPLLEANAISKQDYVNAVASF
                     KQADADVASARAAVTTAQINLGYASVTAPISGRIGRALVTEGALVGQGEATQLALVQQ
                     IDPMYVNFTQSTTEVLRLRKAIEAGRLQRSGATAATSVQVVLEDGSVYGAPGKLLFSD
                     LTVDATTGQITLRAEVPNPSGLLLPGTYVRVRLQQAQASEAVLLPQQAVSRSPLGDSV
                     MVVAADGKFGPRSVKIGGAQAGQWIVLDGLKAGEQVMVDGFQKLRGQTLVNPVPWQPA
                     ASAASGPAVDPARAAASSASSAN"
     gene            10447..13590
                     /locus_tag="PKV56_05030"
     CDS             10447..13590
                     /locus_tag="PKV56_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX67210.1"
                     /translation="MAQFFINRPIFAWVIALFILVIGGVAITQLPVAQYPPVAPPSIV
                     VNVAYPGASAKTLEESVISVIEREMNGSPGLIYMESVSQADGTGAITLSFQPGTSPDL
                     AQVDVQNRLSRANPRLPQAVTQQGVRVDKANPNFLLFAILSSETAAYDPVALGDYASR
                     NVLPQIQRVPGVGQAQLFGTERAMRVWIDPAKLVGLRLSSADVTAAIAGQNAQVASGS
                     IGDLPNTAGQPVFATVVVKGQLNSTEQFGDIILRANADGSSVRLKDVARIELGAQSYG
                     TRIRLNGEPSTGIGVQLSPTANAVATATAVKKRIDELSRYFPPGVKASIPYDSSRFIS
                     ISIGQVVETLFEAVILVFLVMYLFLQNIRYTLIPTLVVPIALMGSLSVLLALGFSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLSPLDATRKAMGQISGAIVGVTVVLI
                     SVFVPLAFFSGAVGNIYRQFSAVMVSAIGFSAFLALSLTPALCATLLKPVEAGHHHAK
                     TGFFGWFNRGFSRTAKGYEGVVARILTRAARYLIIYAAIIGAVVVMFGRLPTSFIPNE
                     DQGNILVNVQLPPGATQGRTTEVMKQAEAWMLKQPDVQSMVSVLGFSFSGTGQNAALG
                     FVTLKDWDEREGAEHTAQALVARAFGALGQIRDAFIFPVSPPAIRELGRANGFAMRLQ
                     DRGGNGHDALLAARNQLLGMAAKSTLLVSVRPDGLEDAPQLQLDVDRDKAGALGVTFG
                     AINTTLSTALGSSYVNDFPNAGRMQRVVVQADAPARMQPDDLLQLNALNSAGQPVPLA
                     SFVTTRWINGPMQTVRYNGYPAMRIQGEPAKGVSSGAAIAEMERLAGQLPPGFAYEWT
                     GQSREEKLSGSTALFLFAFSLLAVFLCLAALYESWSIPLAVILVVPLGVLGVVLGIGW
                     RGMENDVFFKVGLITIIGLSAKNAVLIIEFAKELHAQGKGVVEAVLEAAHLRFRPILM
                     TSLAFILGVLPLVLASGAGSASQRAIGTGVMGGMLTATLLAVFFVPVFYVVVMRVFSR
                     RPAIADAQPIAGPGDAP"
     gene            13587..15035
                     /locus_tag="PKV56_05035"
     CDS             13587..15035
                     /locus_tag="PKV56_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOX67211.1"
                     /translation="MTLPTAFVHPLRSGTLALLVAGIAGCGSMIPEFVRPAAPVPAAF
                     PGTGTQAVPDSTPAADLEWQRYFSDPQLQRLIDLALKNNRDLRVAVLNIEQASAQYQV
                     RRADELPTVNGGITGSRSPRVGGGSGSSINSTYTAGLSVTAYEFDFFGRVRSLSQAQL
                     AQFAGTEEARKTVQISLVASVANAWLGLLADDELLELTRRTLASREESMKLTRLKFDH
                     GASSEIDLRQAESLLEGARVALAQQTRQRAQDENALALLLGQPVPPDAVSAATPLGAS
                     ALAQTVLPDVPAGLPSELLSRRPDIRQAEQQLIAANANIGAARAAFFPRITLSTSVGI
                     ASTQLSDLFKGGSLAWSFAPQLVLPIFDAGRNQANLDAAQVNRDIATAQYEKAVQSAF
                     REVADALAGRATLGEQLRAQQAQAAAEQVRTKLADLRYRNGASSYLDVLDAQRSLFTA
                     QQAVVQAQAAERQNRVTLYKVLGGGWKDAATQ"
     gene            15395..16675
                     /locus_tag="PKV56_05040"
     CDS             15395..16675
                     /locus_tag="PKV56_05040"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOX67212.1"
                     /translation="MNAPDLQAVIARMGASARAASAHMAAAPTAAKNRALIALARLLR
                     ADVDALVAANALDLDAATAAGTAGPLLDRLKLTPKIIETVAEGCEQIAAMPDPIGEIT
                     HLRQRPSGISVGQMRVPLGVFGMIFESRPNVTIEAASLAIKSGNACILRGGSEAIHSN
                     LALWKRVQAALAEAGLPPDAVQLVETTDRAAVGHLITSPESVDVIIPRGGKGLIERIS
                     REAKVPVIKHLDGNCHVYVDATADMALALKVTDNAKTQKYSPCNAAESLLVHAAIAAE
                     FLPRIGAVFAAKGVEMRGDAASLALLSHVPGAHLVQATEQDWFEEYLAPIISVAVVPD
                     LGAAIAHINRYSSHHTDAILTEHHPSAMRFLREVDSSSVMVNTSTRFADGFEYGLGAE
                     IGISTDKLHARGPVGLEGLTSLKWVVLGQGEVRT"
     gene            16710..17255
                     /locus_tag="PKV56_05045"
     CDS             16710..17255
                     /locus_tag="PKV56_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOX67213.1"
                     /translation="MSDTQIATLVGCAVLVFWTVGAYNRLTALRHDIGKAFVPVDAQV
                     QHRHTLLLQWAEGLRALLDQAPQLHDALLMACGQLQAACDALRVRPSARSAATSLRLA
                     EEALSSARSRLLAELPARQQGLSGLEIEANSEQLAAADNTLAFARAQFNTATQTYNDA
                     IRQIPTRLVASVFGFQAAGTL"
     gene            17252..18067
                     /locus_tag="PKV56_05050"
     CDS             17252..18067
                     /locus_tag="PKV56_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67214.1"
                     /translation="MKPAASTEEQDALARLSRTQGPASLGAAVLSLIAQWDTGSTASR
                     ALRAAWNSETQGTPNAEAIDADVALLSAATRLPCLEALLQRVRRGSKDDRRALLQATR
                     RVMAARSPTPPIYRLLWLAMRRQLGERPPAADLPAASNDLTDLPTPMRSHIAVVTAHL
                     SRLVPGAAGSGDRDGAAWYALVLERLMPQAAAMPNWPDGDALAHALLEVQTLPWMLRP
                     VLVRAWVDAASQVGPRIGLLPTAADALRLVAGLLDSPLPPELARRYTEPAWGP"
     gene            18074..18493
                     /locus_tag="PKV56_05055"
     CDS             18074..18493
                     /locus_tag="PKV56_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX67215.1"
                     /translation="MLNAHEHMRFAAESPPDDALHSDTFDDWSFAMFLSNSWQWVGLG
                     WISMAAVAPVQAQDAQSLRLSSLAATCANCHGTGGRSIDDAAVPGLAGMPASYAVDQM
                     KAFKSGARPATVMHQIAKGYSDVQIEQIAVYFAAQKK"
     gene            18504..19814
                     /locus_tag="PKV56_05060"
     CDS             18504..19814
                     /locus_tag="PKV56_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX67216.1"
                     /translation="MTLDSRRRELLKLASATGGLATLGLSGCASGPGAIPDKARVLVV
                     GGGYGGATAAKYLRLFSEQKIDVVLIEPGEAFVSCPLSNLVLGGSRQLGDLTVPYASL
                     TRAHGITVVRDTVASIDATQKLAVLSSGASIRYDKLVLSPGIDLMWDSVAGLKAAHTS
                     GRVLQAWKAGPETAALRRQLEAMPDGGVYALAIPEAPYRCPPGPYERACQVASYFKAA
                     KPRSKVLVLDANPDVTSKGGLFKKVWAEQYKGMVEYRPQHKAVAVDASAASGINVLKF
                     EVQDDVQADVLNVLPPMRAGAIAVQTGLANSNARWCGVKYLNFESTVAKDIHVLGDSI
                     QLAPLMPKSAHMANSHAKVAAAAIVAELSGWEINPAPMLTNTCYSFVDDNTVVHVASV
                     HEYVAAEKTFKTVAGAGGLSAAPNELEGRYAWNWARTIWADTLG"
     gene            complement(19842..20267)
                     /locus_tag="PKV56_05065"
     CDS             complement(19842..20267)
                     /locus_tag="PKV56_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOX67217.1"
                     /translation="MLVRLMYASRAANSVNQNELVAILKKSKANNAEVGVTGVLCFSS
                     GIFLQVLEGGRLQVSALYNKIATDPRHHDVVLLSYEEVEERRFAGWSMGRANLARLNP
                     ALVMKYSETALLNPYAVSGKMSMALFNELVASAAIICES"
     gene            complement(20301..21032)
                     /gene="folE"
                     /locus_tag="PKV56_05070"
     CDS             complement(20301..21032)
                     /gene="folE"
                     /locus_tag="PKV56_05070"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOX67218.1"
                     /translation="MPAKSSPAIFKSEEQLARLSASERIRYRLVGANCRYHANDNISA
                     FIEGGEMEELQAEVEAKMQEVLQALVIDTESDHNTHDTAKRVAKMFLTEVFAGRYTPM
                     PAVTEFPNFSRLNELMIVGPITVRSACSHHLCPIMGRVWIGILPNEHSNLIGLSKYAR
                     ICDWIMSRPQIQEEAVTMLANELQDRVKPDGLALVMEADHFCMHWRGVKDTEAKMINS
                     VMRGSFLKDVNLRREFLSLLDRKST"
     gene            21149..22504
                     /gene="rsmB"
                     /locus_tag="PKV56_05075"
     CDS             21149..22504
                     /gene="rsmB"
                     /locus_tag="PKV56_05075"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOX67219.1"
                     /translation="MKSPAHITASGVTADKPTLARLLDHTADAIEAVRAGRSLTGALA
                     RCPAEVRPGVHALSSHVLRRLGQALAAREWLVPKAPPKGVDALLLSALALSWPDGEAP
                     YADHTLVDHAVTAARRRHAASAAFVNAVLRRFLREQPAVLEAAQRTLPGKYNHPDWWV
                     EQLQSDWPEHWQALLAANDRHPPMTLRVNTRRVSVSAYLARLSVAGLPGRPIGTQGVM
                     LNTPCPVSRLPGFADGDVSVQDAAAQLAAPLLLAGAALPPGARVLDACAAPGGKTAHL
                     LELADLDVLALDHDAERLTRVGETLDRLHLHARMQAGDASQPSTWWDGKPFDAILLDA
                     PCTASGIVRRHPDVRWLRRPSDVPALAATQARLLDALWPLLSPGGRLLYSTCSVFKAE
                     GQDQIDAFLQRQSDASRVVDPPSPGHLLPLPDNQPPAENPGSAARADGFFYALLQKNQ
                     V"
     gene            22558..23127
                     /locus_tag="PKV56_05080"
     CDS             22558..23127
                     /locus_tag="PKV56_05080"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOX67220.1"
                     /translation="MFARFLSLWATCLLALTLWIGSLPAWAETPAPELSQFAVSRNEE
                     GLLLDFAVRFDLPHSVEDALLKGVPLHFVADASLFRERWYWRDQRVSRVTRTWRLAYQ
                     PLTRTYRVSFGGLNQSFDSLADAVASVRRMGRWKLTEPGQIEEGEQYYVEFSYKLDTT
                     LLPRPMQIGIGGQPDWTLQLEKTQRVTTP"
     gene            23212..25524
                     /locus_tag="PKV56_05085"
     CDS             23212..25524
                     /locus_tag="PKV56_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67221.1"
                     /translation="MTKANRWAWIVSLVAATGAGLVLTFLLSIATNNRTFYERHYTWL
                     FWVNMVVAGLLVLVIGIAAVRLFVRVQRGKFGSRLLLKLAAIFALVGVLPGVLIYTVS
                     YQFVSRGIESWFDVKVEGALDAGLALGRGTLDAQVNELANKTQFAAERLGESAGEVQP
                     LSLERLREQLAAQDVAIVNGIGQVLLAANRSAAFQMPDRPALSLLRSARSGRVAAQLE
                     GLDDDASGAGTGTSSGGPARIRALAILPSADIALAQQERYLLVTTLLPAALATNALAV
                     QAAYREYQQRSLAREGLRKMYIGTLTLTLILAVVGALLLAVTLSNQLARPLLLLAEGV
                     RQVAKGDLGVKQVFDSRDELGGLTRSFADMTEQLSEARSLVQRSVSQVEGARANLQTI
                     LDNLTAGVIVFDRDGLIDTVNPGATRILKLPLSAYRGRRLDEVAGLDGFAASVWQRFE
                     LHADSLEAGERGHWQEAFELQTAPNRDSLTLLVRGAAMPQGARLMVFDDITEVVSAQR
                     IEAWSEVARRLAHEIKNPLTPIQLSAERLQHKLSAKLEGADQAMLVRSVGTIVTQVQA
                     MKTLVNEFRDYARLPAAQLKPLDLNALVNEVLTLYATAQEAGTLGFVPAHDLPLIIGD
                     PTQLRQVIHNLVQNALDAATDPANSHTPPQVRVRTEAGRTDQGAVRVVRLHVIDNGPG
                     FPDKVLKRAFEPYVTTKSKGTGLGLAVVKKIADEHGARVRISNLISSETEGGAGEDAI
                     HGAQVSLSFSKFAPGDAHTAAAASDSAPAH"
     gene            25531..26220
                     /locus_tag="PKV56_05090"
     CDS             25531..26220
                     /locus_tag="PKV56_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67222.1"
                     /translation="MATILVVDDELGIRALLSEILTDEGHSIELAENAAQARAARERG
                     QPDLVLLDIWMPDVDGITLLKEWNANALLTMPVIMMSGHGTIDTAVEATKNGAMAFLE
                     KPITLQKLLRAVEQGLVKPAAAARAAANVTALRGDLAHASVTTHSSHVALASLSMTAG
                     PQPEQSFDLDRPLREARDAFEKSYFEFHLAQENGSMTRVAEKTGLERTHLYRKLKQLG
                     VDLSRSKRGSL"
     gene            26258..26333
                     /locus_tag="PKV56_05095"
     tRNA            26258..26333
                     /locus_tag="PKV56_05095"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26291..26293,aa:Phe,seq:gaa)
     gene            26587..26715
                     /locus_tag="PKV56_05100"
     CDS             26587..26715
                     /locus_tag="PKV56_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67223.1"
                     /translation="MTTHLARDTRDKLAKIYAAGIAVFIGIFGFVTAFAVFVSHLK"
     gene            complement(26835..27713)
                     /locus_tag="PKV56_05105"
     CDS             complement(26835..27713)
                     /locus_tag="PKV56_05105"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX67224.1"
                     /translation="MSAAVSAPADLMGMSDSEIDALVKNIGIPPRPATLAELQKEIGR
                     EEPDLRRVAQLVAGDVALTTAVLRIVNSPAYGLRRHCETVDQAIAMIGLKQVTVVVTG
                     LMLRKVLRTDGPQLTRFWDVSAKRSHAMACLARGLGGVDADIAQSFGLFCDVGIPLLI
                     NRFPDYGATLKSCNDEPERRFTEVEQTHHQTDHALIGGLMGRTWSVSQIVCQAIRSHH
                     DYGIFQDPRIPDAVTRLVAMGLVAEVAIQRFSGLNSSAEWNKGGDCAAGALMLSDNDV
                     EEWTERLLEQFSLGIS"
     gene            28059..28616
                     /locus_tag="PKV56_05110"
     CDS             28059..28616
                     /locus_tag="PKV56_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67225.1"
                     /translation="MQTKTSAFRLLAPLAFALCIASASAPAAAQSTVLTFNDLALVSP
                     ATVGASMPDGYGGFDWGGRYYYMAPTAASGNTTYVAMGSGASTWIRRTDSAAFYFDGA
                     DFWSRRGLDANGDFYFVLSLKGKTVYNGLTAKKGGRMLFTGAPTLLKPDYAGPIDLIA
                     IAFSGNGKDWNHLAFDNFRFRAIAP"
     gene            28795..29460
                     /locus_tag="PKV56_05115"
     CDS             28795..29460
                     /locus_tag="PKV56_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HOX67226.1"
                     /translation="MTHHPPDLESRRQYSPAAERNAAPILAQLQRLLPPQGSALEIAS
                     GTGQHAAHFAAGLPGWRWQPTDADPHALASIDAWCNDLDNVRPALHLDVLVEARDSPN
                     SELPEKVDAIYCANMLHISPWETCAALMLLASRHLAPRGMLLLYGPYREDPAPTAPSN
                     LAFDADLRSRDPAWGLRRLADVLQQANAVELRLRERVAMPANNLLLVLERSLGPTKDS
                     AWA"
     gene            29457..29954
                     /locus_tag="PKV56_05120"
     CDS             29457..29954
                     /locus_tag="PKV56_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOX67227.1"
                     /translation="MKYLTGYAPEVLNRVSELIAQGRLGESLRQRYPEPHAVRTDPAL
                     YDYVMELKNRFMRSAPPVAKVAYDNRLQLLHNALGTHSAVSRVQGGRLKAKREIRVAS
                     LFKETPGAFLKMIVVHELAHLKERDHDKPFYALCTHMEPAYHQLEFDLRLWLTARELD
                     GSRPG"
     gene            complement(29966..30448)
                     /locus_tag="PKV56_05125"
     CDS             complement(29966..30448)
                     /locus_tag="PKV56_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67228.1"
                     /translation="MTEPAGEDSTVHEPRLWRDSGWTARVIKNEDDEGWAVEMVKQGE
                     VEPALVGPWTMGRDKKNPKPLDNTAFNTLVKTASEVLRRHEQQLHAQLHKSLVVQADS
                     GRITVTLDIVPDEDDPHAVLAAADAGGQLLAQVRVSAGFRLSSGSADAWIANEFRRPG
                     "
     gene            30637..31011
                     /locus_tag="PKV56_05130"
     CDS             30637..31011
                     /locus_tag="PKV56_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67229.1"
                     /translation="MHVRCLLLTTGLALVVAALPAAAETRAPAPAARASAPVAVKPGP
                     RLMSPAEMNQNASPPGELRPENAVTPQIVIPLRNQSAVPPKPDKRNLKPAATGGVNDA
                     AARCDALTDAAARAACRESAKR"
BASE COUNT         5271 a        10512 c        10279 g         5174 t
ORIGIN      
        1 cacgcgcagc gacatcgagg tcttcctgcc gccgatcggc gggcagacgc tgtacatcct
       61 cggtgatccg cgctccctcg ccgatccatc gatcacgctg acggcgcgcg tccacgacga
      121 atgcaacggc agcgatgtgt tcggctccga catctgcacc tgccggccct acctgacgca
      181 cgcgatcgag gaatgcatac gcggcgcgca gcagggcggc gtgggcctga tcgcctacag
      241 ccgcaaggag ggccgcgccc tcggcgaggt gacgaagttc ctcgtctaca acgcacgcaa
      301 gcggcagctc ggtggcgaca gcgccgacaa gtacttcctg cgcaccgagt gcgtcgcagg
      361 cgtgcaggac atgcgattcc aggaattgat gcccgacgtg ctgcactggc tcggcatccg
      421 aaaaatccac agattggtca gcatgagcaa cgacaagtac gacgcgatca cgggcagcgg
      481 catcgaggtg ggcgaacgca tcaagattcc cgacgagctg gtaccggccg acgcccgcgt
      541 cgagatcgac gcgaagatcg ctgccggcta tttcaccgac ggtgcggtac cgagcgccgc
      601 cgacctggcc gccaccaagg ggcgcgatct tgagtgagat cgtgctggcc tcaccgcatg
      661 gccgcgatgc cgtcagcgtg ctgcgcgacc cggccaccat ccgcaggcgc tgcgcggcag
      721 tcacgcaggc gatcagcgaa ggccgatcgg ccaacttcaa gctcgatcgc agccggctcg
      781 acgaggtcgc ggcccgtgtc gagcgcgtga cacgcgccgc ctatcccgag ctgcggattc
      841 cgtttcacag ccggtggcgg catttcgcgt cgggcggcat cgatcgcaag gcgtcgatgg
      901 acgcgcagat ggccgaccgc agcgtcgccg accaggcccg cgcgcgcttc gacctgacgg
      961 tggtcagcgt gctgctcgac gccggcgctg gtccgcaatg gcgctacgtc gaagacggga
     1021 ccgggcagac cttcagtcgc tccgaaggac tcggtatcgc gagctggcat gcgttccagc
     1081 aagggctgtt ctcgagcgcg ccgggcgatg atccgctgcg tgcggatgcg gcagcgctgc
     1141 agcggctcga tgcgagtacc ctgcggcgga gtttccaggt gaccccgggc aacccgctgg
     1201 tcggtctcga agggcgcgtt gcgctgctga accggctcgg tgcggccctg gcagccgacc
     1261 agggcatggc ggggcgcccc tcagccttgt tcgaccggct gactcagtct gcctcggtct
     1321 cggatggaac cgccgaggtc gatgccggac aggttctgcg ggccgtgctg gatgcgttct
     1381 cgtcgatctg gctcaccggc agcatgctgc agggtgtgcc gctgggcgac gtgtggcccc
     1441 accgctgggc gggcggcgaa tcgaaatccg aaggcggcca cggaaccacc gacggctggg
     1501 tgccgttcca caagctgagt cagtggctga gctactcgct gctggagccg ctgatctggt
     1561 ccggcgcgca cgtgaccggt ctcgatgcgc tgaccggcct gcccgagtac cgcaatggcg
     1621 ggctgctgct cgacggcggc gtgatcgtcc cgcgcgatcc gcgcaacctg gcacgccgct
     1681 ggacgccggc cgatgaattc atcgtcgagt ggcgcgcgct gacggtgtcg ctgatcgacg
     1741 aactggctcc gctggtgcgc gcgcgactgg gcaagaccgc cgaagagatg ccgctggcct
     1801 gcatcctcga aggcggcacc tgggcggcgg gccgggagat tgctagggaa ctgcgagacg
     1861 gatcgccccc gctgtcgatc gacagcgacg gcacggtttt ctgattcact gaaacgaggc
     1921 gcccatgacc gctccggtcc accatgtcac ccacccgctg atccagcaca agctcacgct
     1981 gatgcggcag aaggactgca gcaccaacag cttccggcgc ctgatgagcg agatcagcat
     2041 gctgatggcc tacgaggtca cgcgcgacat gccgacgcag ctcatcgaca tcgagacgcc
     2101 gctggaggcg atgaagtcgc ctgtgatcga cggcaagaag acggtgttcg tcagcatcat
     2161 gcgtgccggt ggcggcttcc tggacggcat gctgacggtg gtgccgggcg cgcgcatcgg
     2221 ccacatcggg ctctaccgtg acccgaagac gctggtcgcg gtcgagtact acttcaagat
     2281 gccttcggac atgcacgagc gcgacgcgat cgtcctcgac ccgatgctgg ccacgggcaa
     2341 ctcggcggtg gcagcagtgg accggctgaa ggagacgcag ccgcggtcga tccgctttgt
     2401 ctgcctgctg gccgcgccgg agggcctgaa ggccttccag tccgcgcatc ccgacgtgcc
     2461 ggtctacacc gcagcgatcg accgcgaact gaacgaccac gggtacatcc tgccgggcct
     2521 gggcgacgct ggcgatcggt tgttcgggac caagtgagca tgctgatccg caacgccacc
     2581 ttgcccgacg ggagcaccgg cgtcgacgtg ctgctgcgcg acggacgcat cgccggcatc
     2641 ggcacgcagc tggcatgtcc cgagaacgcg cgcgaggtcg atgcgcgtgg ccacctgctg
     2701 gcgccatcgt tcgtcgatgc gcacttccac atggacgcga cgctgagcta cggcctgcct
     2761 cgcgtcaatg ccagtggcac cctgctcgag ggcatcgcgc tgtggggcga actgaagccg
     2821 cagctgacgc aggacgcgct ggtcgctcgc gcgctgcagt actgcgactg ggccgtcgcg
     2881 aagggcctgc tggcgatccg cacgcatgtc gacgtctgcg acgatcggct gctggcggtc
     2941 gaggcgctgc tgcaggtgaa gcgccaggtg gcgccctacc tcgacctgca actggtggcg
     3001 ttcccgcagg acggcctgct gcgttcgccg aacgcgctgg ccaacctgaa gcgtgcgctc
     3061 gatctcggcg tcgacgtggt cggcggcatc cctcatttcg agcgaacgat ggccgaaggc
     3121 accgaaagca tccgcatcct gtgcgaactg gcggccgagc gcggcctgcg cgtcgacatg
     3181 cactgcgacg aaagcgacga cccgctgtcg cgccacgtcg agacactggc cttccacacg
     3241 cagcgcctcg ggatgcacgg ccgcgtcacc ggctcgcacc tgacatcgat gcattcgatg
     3301 gacaactact atgtgtcgaa gctgattccg ctgatggccg aggcgcaggt gaatgtggtc
     3361 gccaacccgc tgatcaacat caccttgcag ggccgccacg actcctaccc gaagcgacgc
     3421 ggcatgacgc gcgttcccga actgatggct gcaggcctga acgtcgcgtt cggccacgac
     3481 tgcgcgatgg acccgtggta tccgctcggc tcggccgaca tgctggaggt cgcgcacatg
     3541 ggcctgcatg tcgcgcagat gacctcacca aaacagatgg aagcctgctt cgacgccgtc
     3601 accgtctgtg gcgcgaaggc catgggtctc gaccggcacg gcatcacggt cggttgcgac
     3661 gccgatctgg tgctgctgca ggcgcgctcg gtgatcgagg cgcttcgcct gcggccgaac
     3721 cgcctgctgg tgctgcgcaa gggaaagatg gtggcttgca gcgctccggc gatgtcccag
     3781 ctcgacctgc cggatcgtcc ggccagcgtc gactggacgc tgcgacgctg agtgacaagt
     3841 cgaatgaggc acctccgcaa accacggatc gggacttacg aagtgaaaag tgccatcgtt
     3901 agaatgaaat ttgcatctgc cagtccagcc tcactccgca cgcggcgatc ccctcgtcgc
     3961 tccccgacat gaaactcatc ggctcactga ccagccctta cgtgcgcaag gttcgcatcg
     4021 tgctggccga aaagaagctg gactaccacc tcgaggtcga ggacgtctgg ggcaacgacc
     4081 gcatccttgc ttcgaacccg ctgggcaagg tgccttgcct ggtgatggaa ggtggcgaag
     4141 cggtgttcga ttcgcgtgtc atcgtcgagt acgtcgacac gctgtcgccg gtgggacgac
     4201 tgatccctga gcgcggccgc gagcgcgcgg aagtgcgcac atgggaggct ctggccgacg
     4261 gtgtgctcga cgcggccatc tcggcacgac tcgaacagac ctgggccggc cgttcggaag
     4321 ccgaacgcag cagcgcctgg atcggtcgac aaatggacaa ggtgaaccat tccgtcgccg
     4381 cgatgagcaa gggcctggcc gacaagccct ggtgctgcaa tggcaaccac ctgacgctgg
     4441 ccgacatcgc agtcggctgc gcgctgtcct atctcgattt ccgcttcccg cagatcgact
     4501 ggcgcagccc gcaccccaac ctggtgcgct tgtcggacaa gctttgcggc cgccccagct
     4561 tcatcgacac ccagccgccc gcggcttgag cttcgggcgc tgacccggcg cgcagcggct
     4621 cgtcacatcg ccacgccaaa gctttccgcc tgcgccatcg gccagtacag ccggtacacg
     4681 ctgtgggtca ggtcgccagt gagcagcgat ccgggtttca cgaacggcag caggttgctc
     4741 agcagcttga cctcgtgatc gttggtgcgc cgcacgatgt gggcggcggt gatggcgccg
     4801 ggatgatcga gtccggcggc ctgcaccagc tccttcagcg ccttgagggt gctctggtgg
     4861 aaattgaaga cacggtcggc cttggtcggc accaccaggg cctgctggcg atgcggatcc
     4921 tgcgtgctga cgccggtcgg gcaatcaccg gtgtgacagg cctgcgcctg gatgcagccg
     4981 agcgcgaaca tgaagccgcg cgccgagttg caccagtccg cacccagcgc catgttgcgg
     5041 gcgatgtcga aggcactgac caccttgccg gcacagccga tcttgacgcg gtggcgcagg
     5101 ttcaggccga cgagcgtgtt gtgcaccagc agcagcccct cctgcagcgg cgcgccgacg
     5161 tgatcggtga actcgagcgg cgccgcaccg gtgccgcctt cgccgccgtc gacgacgatg
     5221 aagtcaggcg tgataccggt ggccagcatg gccttggcga tggcgaacca ctcccacggg
     5281 tggccgatgc acagcttgaa gccgacgggc ttgccgcctg acaacgcacg caggcggtcg
     5341 acgaactgca tcatctcgat cggcgtggag aacgcgctgt ggctggccgg gctgacgcaa
     5401 tcgacccagg gcggcacgcc gcgcgcttcg gcgatctccg gcgtcacctt cggccccggc
     5461 aggacgccgc cgtgtccggg tttggcgccc tggctcagct tgatctcgat catcttgacc
     5521 tgcgccagcc gggcgttggt cgcaaacaga tccgggttga agctgccgtc ggggtggcgg
     5581 cagccgaagt agcccgagcc gatctcccag atcaggtcac cgccgccgtc cggatcgggg
     5641 ccccgatggt ggcggctgat cgagccttcg ccggtgtcgt gcgcgaagcc tccgcgcttg
     5701 gcgcccgcgt tcagcgccag gatcgcattg gcggacaagg caccgaagct catcgccgag
     5761 atattgaaga cgctggccgt gtaaggctgc gtgcatgggg ttccatgagc accctgggag
     5821 tgcgcgggat cgccatggcc gccaccaatg accacccgga aatcatgggt cggcaggtgg
     5881 gtcgggtgca tcgaatggtt gatccactcg tagccctggg cgctgacgtc cttctgcgtt
     5941 ccgaagggcc gcttgtcctg gtcgcccttg gcgcgctggt agaccagcga gcgctgctgg
     6001 cgcgagaacg gcgccgcttc gttgtcaccc tcgatgaagt actgcctgat ctccggtcgg
     6061 atgaactcga acaggtagcg catgtgcccg atgaccgggt agttgcgcag gaccgagtgc
     6121 gtgccctggc gcgtatcgcg caccccgagc agcaccagcc ccgcgaagat cgacggcagc
     6181 acccagtccc acccgctggg catgcgcatc agcagcgaca gagacaggct gacgaccagt
     6241 cccaccgcac aaaggaacca cgcggagtac ctcagcggga agtgatggtt cagcttttcg
     6301 agccaggttt gcatcgcgcg tcctcgaggc gagccgcgga cgcggtgcgc ccatcgaccc
     6361 gaaactcatc gtatcgaccg gcgatggcgt ccgattcgac gaaaagaccc cgtaagtcgc
     6421 gtcggacgcg gcgacctgac tccgggtgtt cccgaacccc ctgtcgcgac gatggaggcc
     6481 ggcatgcgct cgctatactg ccgcccagcg ctgatttgct ccggcttgcg ggcgctctac
     6541 aaacccagct aaatacagga ccgacgggcg cttcccgtca caccaccacg atgcttcaac
     6601 ccgctacgcc gccggcccgt gcggcctgtc gacggattca ttcatgagtt cgctgggaca
     6661 cgttcggccg cgatcgatgt cgttcgcgga gcctctgacg ctgcgctgcg gcgcggtgct
     6721 gcgcgattac accctcgtgt acgagaccta cgggacgctg aacgcggagc gcagcaacgc
     6781 ggtcctggtg tgccacgccc tgaacgccag ccaccacgtc gccggcacct acgagggcga
     6841 cccgaagagc accggctggt gggacaacct gatcgggccc ggtcggccgc tggacaccga
     6901 tcgcttcttc gtcatcggtg tcaacaaccc gggatcgtgc ttcggatcga ccggcccgac
     6961 ccacatcaac ccggcgacgc agcaaccctg gggcgtggat tttcccgtga tgacagtgga
     7021 ggactgggtc gacgcgcagg cgcggctctg cgatgcactg ggcatcgagc aactcgcggc
     7081 ggtgatcggc ggaagcctgg ggggcatgca ggcgctgtgc tggtccatgc gatatccatc
     7141 ccgattgcgg cactgcatcg cgatcgccac cgcacccaac ctgtctgcgc agaacatcgc
     7201 cttcaacgaa gtcgcgcggc gcgccatcgt gaccgacccc gacttccatg acggccacta
     7261 cctggcccac ggcgtggtgc ccaagcgcgg tctgcgcgtg gcacggatga tcggccacat
     7321 cacctacctc agcgacgaca cgatggacgc caagttcggc cgcaccctgc gcgccgcgat
     7381 cggcgcagag gtcgtccagc gctccggctc gccgcgctac accacgctgg acatcgagtt
     7441 cgagatcgaa agctacctgc gccaccaggg cgacaagttc agcgagtact tcgacgcgaa
     7501 cagctacctg ctgatcaccc gcgtgctcga ctacttcgat ccggcgcgcg agcacgatgg
     7561 cgacctgtcc gccgcattcg ccgcagccac tgccaggttc cagctggtga gcttcacgac
     7621 cgactggcgc ttctcgccgg cccgctcacg cgagatcgtc aaggcgctgc tcgacaaccg
     7681 gctcgacgtc agctatgccg agattgccgc cccgcacggt cacgacgcgt tcctgctcga
     7741 cgactcgcgc taccacgggg tgctgcgcgc gcgcttcgag cgcatcgcgc gcgagatcga
     7801 cgccggctca cacgctgacc tggcacggcc tggcctgggg attgcgacat gagcgccgga
     7861 aacgacctcg atctgatcgc gggcctggtg ccgaccggct cgagggtgct cgacctgggc
     7921 tgcggctcgg gcgaaacgtt ggcctacctg cgcgaccggc gtcaatgcac cggctatggc
     7981 atcgagatcg cggacgccaa cgtgctggcc tgcgagcagc gcggcgtcaa cgtgatccag
     8041 ctcaacctcg aggaagggct ggcgatcttc gacgaccaga gctttgacgt ggtgctgcag
     8101 atcgacacgc tgcagcacct gcgcaatacc gagcgcatgc tggcggaaac cgcccgcgtc
     8161 ggtcgcatcg gcatcgtcac cttcccgaat ttcgcgcact ggccgaaccg gctcagcgtg
     8221 atccggggcc ggatgcccgt gaccaaggcc ctgccctacg agtggtatga cacgccgaac
     8281 atccgcgtga gcacctacgc cgatttcgag gtgctggcgc gccgctgcgg gctgcgcatt
     8341 ctcgatgcgt tcggcatcca gctcggtgcg ccagtcaggc tgtgcccgaa cctgctggcc
     8401 agtgtcgcgg tgttcaagtt cgagcgggcg tagtcgaagc cggcagcgtc agcccgcaca
     8461 ggtaggcgtc gagcgcctgc tcgccgacct ggaccagatc gaacgcggac gggtcgagca
     8521 gccagttctc gatcagtccg gaggtcagcg catagaggcc gatggcggtc gcgagcgcag
     8581 gcgtgcggcg gttcaggcga cccgtcgcca tcgcgcgctc cagacccacc tccacattgc
     8641 gtatcaggcc ggcccgattg ttgacccggc gatcgcgcac cacgagcagc tcgtcgacat
     8701 attcgacctt ctgcatcttg atcacgaaca ccctctgcag ttgcgggtct gccaccgtct
     8761 tgcgcagcgc attcagcaga ctgtttcgca cctccaggac ggaatcggcg gtgtccggat
     8821 cggcagatcg gttgatggct tcgtcgagcg gcagcgtcac tcgcttgaac atcgcattga
     8881 cgagatcggc cttgttctcg aagtgccagt agatggcgcc gcgcgtcacg ccggccgcct
     8941 gcgcgatctc ctgcagcgag cagcgggaca cgccgcgctg ctcgaagacg agctctgccg
     9001 cgtccaggat gcgatcccgg gtggccagcg cgtcgtcctt ggttctgcgt gccatcaagc
     9061 ccctccggag tccttataca tacaaacacg tatgtatgta gactagccgc ttttcgtgcg
     9121 gatggtgagc cgaatcaacc cgcccgctgc cagcctgttg cgcagaaggc accctcagga
     9181 tgacgttgca tgagcgtgca taaacgaatc ggttttcgtt cttcgatggt gctcgcggtg
     9241 gtgggtgcaa cctggctgat cgcgagcctg tcaggttgtg gtggcaagcc gggcgagaca
     9301 tcgccggctt cggccgccag tgcgccgccc ccggccgtcg gggtgatcac ggtgacaccg
     9361 cagcccgtgg gaatggcgag cgagttgccc ggccggctcg aggcgacgcg gatcgcgcag
     9421 gtgcgcgccc gcgcagccgg catcctgcag aagcgcctgt tccgcgaggg cagcgacgtg
     9481 aaggctggcc agttgctgtt ccagatcgac gccgcgccgt accgcgccca gctgcaaagt
     9541 gcacaggccg cccaggcacg tgccgaggcc aaccgcgaac aggccggtgc gcaggccgag
     9601 cgctacaagc cattgctcga agccaacgcg atcagcaagc aggactacgt caacgcggtc
     9661 gcgtcgttca agcaggccga cgccgatgtc gcgtcggccc gggcggcggt gaccaccgcg
     9721 cagatcaacc tcggctacgc gtcggtgacg gcgcccattt ccggccggat cggtcgtgca
     9781 ctggtcaccg aaggtgcgct ggtcggtcag ggcgaggcca cccagctcgc actggtgcag
     9841 cagatcgacc cgatgtacgt gaacttcacc caatcgacca ccgaggtgct gcgcctgcgc
     9901 aaggccatcg aggccggccg gctgcagcgc agcggtgcga ccgctgcgac gtcggtgcag
     9961 gtggtgctcg aggacggcag cgtctacggt gcaccgggca agctgctgtt ctcggacctg
    10021 accgtcgacg cgacgacagg acagatcacc ctgcgcgcgg aggtgcccaa ccccagcgga
    10081 ttgctgctgc ccggcactta cgtgcgcgtg cgactccagc aggcgcaggc cagcgaggcg
    10141 gtgctgctgc cgcagcaggc tgtgtcccga tcgccgctgg gcgacagcgt gatggtcgtg
    10201 gctgccgacg gcaaattcgg tccgcgcagc gtgaagatcg gtggcgcgca ggcgggtcag
    10261 tggatcgtgc tcgacgggct gaaggcgggt gaacaggtga tggtcgacgg attccagaag
    10321 ctgcgtgggc agacgctggt gaatccggtg ccgtggcagc ctgccgcctc cgctgcttcc
    10381 ggaccggcgg tggatccagc ccgggcggct gcatcgtcgg cgtcgtccgc gaactgaggc
    10441 cgcgtcatgg cgcagttctt catcaaccga cccatcttcg cgtgggtgat cgcgctgttc
    10501 atcctggtga tcggaggcgt ggcgatcacg cagttgccgg tcgcccagta cccgccggtt
    10561 gcacccccat cgatcgtcgt gaacgtggcc taccccggcg cgtccgcgaa gacgctcgag
    10621 gagagcgtca tcagcgtcat cgagcgcgag atgaacgggt ctccgggcct gatctacatg
    10681 gagtcggtca gccaggccga cggcaccggc gcgatcacgc tgagcttcca gccgggcacc
    10741 agccccgacc tggcgcaggt cgatgtgcag aaccggctca gccgcgccaa cccgcgcctg
    10801 ccgcaggcgg tgacgcagca gggcgtacgc gtcgacaagg cgaatcccaa tttcctgctg
    10861 ttcgccatcc tgtcgagcga gaccgccgcc tacgacccgg tggcgctggg tgactacgcc
    10921 tcgcgcaacg tgctgcccca gatccagcgc gtgccgggcg tcggccaggc gcagctgttc
    10981 ggcaccgagc gcgcgatgcg cgtctggatc gatccggcca agctggtcgg gctgcggctg
    11041 tcgtccgccg acgtgacggc cgccatcgcc gggcagaacg cgcaggtcgc ctcgggcagc
    11101 atcggcgacc tgcccaacac ggcgggtcag ccggtgttcg cgaccgtggt cgtcaagggg
    11161 cagctgaact cgaccgagca gttcggcgac atcatcctgc gcgccaatgc cgacggttcc
    11221 agcgtgcgac tgaaagacgt ggcgcgcatc gagctgggcg cacaaagcta cggcacgcgc
    11281 atccggttga acggcgagcc atcgaccggt atcggcgtgc agctttcgcc cacggccaat
    11341 gccgtggcca ccgccaccgc cgtgaagaag cgcatcgatg aactgtcgcg gtactttccg
    11401 ccgggcgtga aggcgagcat cccctacgac agctccaggt tcatcagcat ctcgatcggt
    11461 caggtcgtcg agacgctgtt cgaggctgtg atcctggtct tcctggtgat gtacctgttc
    11521 ctgcagaaca tccgctacac gctgatcccg acgctggtgg tgccgatcgc cctgatgggc
    11581 agcctctcgg tgctgctggc gctgggcttc tcgatcaacg tgctgacgat gttcgggatg
    11641 gtgctggtga tcggcatcgt ggtcgacgac gcgatcgtcg tggtcgagaa cgtcgaacgc
    11701 atcatgagcg aggagggcct gtcgccgctc gacgcgaccc gcaaggcgat gggccagatc
    11761 tccggcgcca tcgtcggcgt gacggtggtg ctgatctcgg tgttcgttcc gctggcattc
    11821 ttcagcggcg ccgtgggcaa catctaccgc cagttctcgg ccgtgatggt cagcgcgatc
    11881 ggcttctccg cgttcctggc cctctcgctg acacccgcac tctgcgcgac gctgctcaag
    11941 ccggtcgagg cgggccacca ccacgcgaag accggcttct tcggctggtt caaccgcggc
    12001 ttctcgcgca ccgccaaggg ctacgagggc gttgtcgccc gaatcctgac gcgcgcagca
    12061 cgctacctga tcatttacgc cgcgatcatc ggcgcggtgg tggtgatgtt cggtcgcctg
    12121 ccgacctcgt tcatcccgaa cgaggaccag ggaaacatcc tcgtcaacgt gcagttgccg
    12181 ccgggagcca cgcaaggccg caccaccgag gtgatgaagc aggccgaggc ctggatgctg
    12241 aagcagcccg acgtccagag catggtcagc gtgctcggtt tcagtttctc gggcaccggc
    12301 cagaacgctg cgctgggctt cgtcaccctg aaggactggg acgaacgcga gggcgccgaa
    12361 cacaccgcgc aggcgctggt cgccagggcc ttcggcgcgc tcggccagat ccgcgacgca
    12421 ttcatctttc cggtcagccc gccggccatc cgcgaactgg gccgcgccaa tggtttcgcg
    12481 atgcggttgc aggaccgggg cggcaacgga cacgacgcgc tgctggccgc gcgcaaccag
    12541 ttgctcggca tggccgccaa gagcacgctg ctggtgtcgg tgcgccccga cggcctggaa
    12601 gacgccccac agctccagct cgatgtcgac cgcgacaagg cgggcgcgct gggggtgacg
    12661 ttcggcgcca tcaacacgac gctgtcgacc gcgctgggct cgtcctacgt caacgacttc
    12721 ccgaacgccg ggcgcatgca gcgcgtggtg gtgcaggccg atgcgccagc gcggatgcag
    12781 cccgatgacc tgctgcagct caacgcgctc aacagcgccg gccagccggt gccgctggcg
    12841 tccttcgtga ccacccggtg gatcaacggg ccgatgcaga ccgtgcgcta caacggctac
    12901 ccggcaatgc gtatccaggg cgagccggcc aagggcgtca gcagcggcgc ggccatcgcc
    12961 gaaatggagc gcctcgcggg acaactgccg ccaggattcg cctacgagtg gaccggccag
    13021 tcgcgcgagg aaaagctgtc aggatcgacg gcgctgttcc tgttcgcctt ctccctgttg
    13081 gccgtcttcc tttgcctggc cgcgctgtac gagagctggt cgatcccgct ggcggtgatc
    13141 ctggtcgtgc cgctcggcgt gctcggcgtg gtgctcggca tcggctggcg cgggatggag
    13201 aacgacgtgt tcttcaaggt cggcctgatc accatcatcg ggctgtcggc gaagaacgcg
    13261 gtgctgatca tcgagttcgc gaaggagttg catgcgcagg gcaagggcgt ggtcgaggcc
    13321 gtgctcgagg cagcgcacct gcgtttccgg ccgatcctga tgacttcgct cgccttcatc
    13381 ctcggcgtgc tgccactggt cctcgccagc ggggccggtt cggccagcca gcgcgcgatc
    13441 ggcacgggcg tgatgggcgg catgctcacg gcgacgctgc tggcggtgtt cttcgtgccg
    13501 gtgttctatg tcgtggtgat gcgcgtgttc agccggcgtc cggcgatcgc cgacgcgcag
    13561 ccgatcgcgg gcccaggaga tgcaccatga cgctgccaac tgccttcgtt caccctctgc
    13621 ggtccggcac gcttgcgctg ctggtggctg gcatcgccgg ctgcggttcg atgattcccg
    13681 agttcgtgcg ccccgcggcg ccggtgcctg cggccttccc cggcacgggc acgcaggcgg
    13741 ttcccgacag cacgcccgca gccgacctgg agtggcagcg ctacttcagc gatccgcaac
    13801 tgcagcggct gatcgacctc gcgctgaaga acaaccgcga cctgcgcgtc gcggtgctga
    13861 acatcgagca ggcgagcgcg cagtaccagg tgcggcgcgc cgacgagttg cccaccgtca
    13921 atggcggcat cactggatca cgctcgcccc gcgtgggcgg cggctccggc agcagcatca
    13981 acagcaccta cacggccggc ctgtcagtca ccgcgtacga gttcgatttc ttcggccggg
    14041 tgcgcagcct cagccaggca cagctggcgc aattcgccgg taccgaggaa gcgcgcaaga
    14101 ccgtgcagat cagcctggtt gcatcggtgg cgaacgcctg gctcgggctg ctggccgatg
    14161 acgagttgct cgaactgacg cgtcgcacgc tggcgtcgcg cgaggaatcg atgaagctga
    14221 ctcggctcaa gttcgaccac ggcgcgtcgt ccgaaatcga tttgcgacaa gccgaatcgc
    14281 tgctcgaagg tgctcgggtc gcgctcgcgc agcagacccg gcagcgggcc caggacgaga
    14341 acgcgctggc cctgctgctg gggcagccgg tgccgccgga tgcggtgtcg gcggccacac
    14401 ccttgggcgc atcggcactg gcgcagaccg tgctgcccga cgtgccggcg ggtctgccgt
    14461 ccgagctgct gagccgccgg cccgacatcc ggcaggccga gcagcaactg atcgcggcca
    14521 acgccaacat cggcgcggca cgtgcggcgt tcttccctcg gatcacgctg agcaccagcg
    14581 tcggcatcgc gagcacccag ctgtccgacc tgttcaaggg cggcagcctg gcctggagct
    14641 ttgcgccaca gctggtgctg ccgatattcg acgcaggacg caatcaggcc aacctcgacg
    14701 cggcgcaggt caatcgcgac atcgccaccg cccagtacga gaaggccgtg cagagcgcct
    14761 ttcgcgaggt cgccgatgcg ctggcgggtc gcgcgacact gggcgaacaa ctgcgagcgc
    14821 agcaggcgca ggccgctgcc gaacaggtgc gcacgaagct ggccgacctg cgctaccgca
    14881 atggcgcgtc gagctacctc gacgtgctcg atgcacagcg ctcgctgttc accgcgcagc
    14941 aggcggtggt gcaggcgcag gcggccgaac ggcagaaccg cgtcacgctg tacaaggtgt
    15001 tgggcggtgg gtggaaggac gctgcgacgc agtgacaaca agtcgcactt gaacggatcg
    15061 ctcatccgag ttcggagcgt gagcactgcg gcgtgccgct gtgttgcgtt catgtcctcc
    15121 gaggcgtgct gtgcccgcct ctcccccttt acttcactcc acacagcgac ccgtcacaga
    15181 gctcctgcca ccatcgaagc aatcgggggg tttgaattca ttcgcatgcg acgacgttgc
    15241 ctgctgtcga gggcgtgggg tgactggcgc agcgaagtag agggggaggg ccggtctttt
    15301 ccggcccgga ggacatgagc ggagccggtc gccccgcgcc atcggcagca ccgcgcgaag
    15361 atcaaggtac agcgaatttg ggacaatcgg tctcatgaac gcacccgacc tgcaagccgt
    15421 gatcgcccgc atgggcgcgt ccgcccgcgc cgccagtgcg catatggccg ccgcgccgac
    15481 ggcggccaag aaccgcgcgc tgatcgcgct cgcgcggctg ctgcgcgccg atgtcgatgc
    15541 gctggtcgca gccaatgcgc tcgacctcga tgcagcaaca gcggcgggta ccgccgggcc
    15601 gctgctcgac cgcctgaagc tgacgccgaa gatcatcgag acggtggccg aaggctgcga
    15661 gcagatcgcg gcgatgcccg atccgatcgg cgagatcacc cacttgcggc agcggccgag
    15721 tggcatctcg gtcggccaga tgcgggtgcc gctgggcgtc ttcgggatga tcttcgagag
    15781 ccggccgaac gtgacgatcg aggccgcatc gctggccatc aagagcggca acgcctgcat
    15841 cctgcgcggg ggctccgagg cgatccactc gaatctggcg ctgtggaagc gcgtgcaggc
    15901 cgcacttgcc gaggcagggt tgccacccga tgcggtgcag ctggtcgaga cgaccgatcg
    15961 cgcggcggtg ggccacctga tcacgtcgcc cgaatcggtc gacgtgatca tcccccgcgg
    16021 cggcaagggc ctgatcgaac gcatctcgcg ggaagccaag gtgccggtca tcaagcacct
    16081 cgacggcaac tgccatgtgt acgtcgacgc caccgccgac atggccttgg cgctgaaggt
    16141 caccgacaac gcgaagacgc agaagtacag cccgtgcaac gcggccgagt cgctgctggt
    16201 gcatgcagcg atcgcggccg agttcctgcc acgcatcggc gcggtgttcg cggccaaggg
    16261 cgtcgagatg cgaggcgatg cggccagcct cgcgctgctg tcccatgttc cgggtgcgca
    16321 tctcgttcag gcgaccgagc aggactggtt cgaggaatac ctggcgccga tcatcagcgt
    16381 ggccgtcgtg cccgacctcg gcgcggcgat cgcccacatc aaccgctaca gctcgcacca
    16441 caccgacgcg atcctgaccg aacaccaccc gagcgcgatg cgattcctgc gcgaggtcga
    16501 ttcgtccagc gtgatggtca acaccagcac gcgtttcgcc gatggcttcg agtacggtct
    16561 gggcgccgag atcggcatca gcaccgacaa gctgcacgca cgcgggccgg tgggactcga
    16621 aggcctgacc tcgctgaaat gggtggtgct ggggcaaggc gaggttcgca cttgatcctg
    16681 cgcgccgcgc cggtttccag gcatcacgga tgagcgacac gcagatcgcc acgctggtcg
    16741 gctgtgcggt gctggtgttc tggacggtcg gcgcctacaa ccggctgacg gccctgcgcc
    16801 atgacatcgg caaggcattc gtccccgtgg atgcgcaggt tcagcatcgc cacacgctgc
    16861 tgctgcagtg ggcggagggt ctgcgcgcac tgctcgacca ggcgccccag ctgcatgacg
    16921 ccctgctgat ggcctgcggt cagctgcagg ccgcctgcga cgccttgcgc gtccgtccca
    16981 gcgctcgcag cgccgcgacc agcctgcgat tggccgagga ggccctgtct tcggcacgct
    17041 cgcggctgct ggccgagttg ccggcccgcc agcaaggtct gagcggactc gagatcgagg
    17101 cgaactccga gcagctcgcg gctgccgaca acacgctggc attcgcgcgc gcgcagttca
    17161 acacggccac ccagacctac aacgacgcga tccgtcagat tcccacgcga ctggtggcca
    17221 gcgtcttcgg atttcaggct gcggggacgc tttgaagcct gcggcttcga ccgaggaaca
    17281 ggacgcgctc gccaggctgt cgcgcactca aggcccggcg agcctcggcg ccgcagtcct
    17341 gtcgctgatc gcgcagtggg ataccgggtc gaccgcttcg cgagcgctgc gcgctgcctg
    17401 gaactccgaa acacagggca cgccgaacgc cgaggcgatc gacgccgatg tcgcgctgtt
    17461 gtcggccgcc actcggctgc cctgtctcga agcgctgctc cagcgcgtgc gccgcggctc
    17521 caaggacgac cgccgggcat tgctgcaggc cacgcggcgc gtgatggccg cgcgctcgcc
    17581 gacgccaccg atctacaggc tgctgtggct ggcaatgcgc cgccagctcg gcgagcgccc
    17641 gccggccgcc gatctgcccg cggccagcaa cgacctgacc gatttgccga caccgatgcg
    17701 ttcgcacatc gccgtggtga ccgcacacct gtcgcgcctg gtacccggcg ccgcaggaag
    17761 cggtgaccgc gacggggccg cctggtacgc gctggtgctt gagcggctga tgccgcaggc
    17821 cgccgcgatg ccgaactggc ccgacggcga cgcgctggcc cacgcgttgc tcgaggtgca
    17881 gacattgccc tggatgctgc ggccggtgct ggtgcgggcc tgggtcgacg cggccagcca
    17941 ggtggggccg cgcatcgggc tcttgccgac ggcggcagac gcgctgcgtc tggtcgctgg
    18001 cctgctcgat tcgcccttgc cccctgaact ggcaaggcgc tacaccgaac cggcctgggg
    18061 cccttagaaa cccatgctga atgcgcatga gcacatgcgc tttgcggccg aatctccacc
    18121 ggacgatgcc ctacattcgg acactttcga tgactggagt ttcgcgatgt tcctgtcgaa
    18181 cagctggcag tgggtgggcc tcggatggat ctcgatggca gcggttgcgc cggttcaggc
    18241 gcaggacgcc cagtcgttgc gactgagcag cctggccgcg acctgcgcca actgccacgg
    18301 caccgggggc cgctccatcg acgacgccgc ggtacccgga ctggccggca tgccggccag
    18361 ctacgcggtc gatcagatga aggccttcaa gagcggtgcc cggccggcca ccgtgatgca
    18421 ccagattgca aagggctatt ccgacgtgca gatcgaacag atcgcggtct atttcgccgc
    18481 ccagaagaag tgaggaccgc gccatgacac tggattcaag acgccgtgaa ctcctcaagc
    18541 tggcctcggc caccggcggg ctggccacgc tgggcctgtc cggttgcgcc agcggacccg
    18601 gcgcgatacc tgacaaggcg cgcgtgctgg tggtcggtgg cggctacggc ggcgcgacgg
    18661 cagccaagta cctgcgactg ttctccgaac agaagatcga cgtcgtgctg atcgagccgg
    18721 gtgaggcctt cgtgtcgtgc ccgctgtcga acctggtgct tggcggcagc aggcagctcg
    18781 gcgacctcac cgtgccttat gccagcctga cccgtgccca cggaatcacc gtcgtgcggg
    18841 acaccgtcgc cagcatcgac gcgacgcaga agcttgcggt gctgagttcg ggcgcgagca
    18901 tccgttacga caagctggtg ctgtcacccg gcatcgacct gatgtgggac agcgtggccg
    18961 gactgaaagc cgcgcacacc agcggccggg ttctgcaggc atggaaggcc gggccggaga
    19021 ctgccgcgct gcgtcggcag ctcgaggcga tgcccgatgg cggggtctat gcactcgcga
    19081 tccccgaagc accctaccgg tgcccgccgg gtccttacga gcgcgcctgt caggtcgcga
    19141 gctacttcaa ggctgcgaaa cccaggtcga aggtccttgt gcttgacgcc aacccggatg
    19201 tcacctcgaa gggcggcctg ttcaagaagg tctgggccga gcagtacaag ggcatggtcg
    19261 agtaccgacc gcagcacaag gcggtggcgg tcgacgcgag cgcggccagc ggcatcaacg
    19321 tcttgaagtt cgaggtgcag gacgacgtgc aggccgatgt gctgaacgtg ctgccaccga
    19381 tgcgcgccgg cgcgatcgcg gtgcagaccg gactggccaa cagcaacgca cgctggtgcg
    19441 gggtcaagta cctgaacttc gagtcgacgg tcgccaagga catccatgtg ctgggagact
    19501 cgatccagct tgcgccgctg atgccgaaaa gcgcgcacat ggccaacagc cacgccaagg
    19561 tcgcggctgc ggccatcgtg gccgagctgt ccggctggga gatcaacccg gcgccgatgc
    19621 tgaccaacac ctgctacagc ttcgtcgacg acaacaccgt ggtgcacgtc gccagcgtgc
    19681 acgagtacgt cgccgccgag aagacattca agaccgtcgc gggcgccgga ggcctttctg
    19741 ccgcgccgaa cgagctggaa ggccgctacg cgtggaactg ggcgagaacg atctgggccg
    19801 acaccctggg atgaccgcgg cgaggccagt tccgtcggcg ctcagctttc gcagatgatg
    19861 gcggccgagg ccaccaactc gttgaacaga gccatcgaca tcttgcccga caccgcgtac
    19921 gggttgagca gcgcggtctc ggagtacttc atcaccagcg ccggattcaa ccgcgcgagg
    19981 ttggcacgcc ccatcgacca gcccgcgaag cgtcgctcct cgacttcctc gtagctcagc
    20041 agcaccacgt cgtggtgcct cggatcggtg gcaatcttgt tgtagagcgc gctcacctgc
    20101 aatcggccgc cttcgagcac ctgcagaaag atcccggacg agaaacacag cacgccggtc
    20161 acgccgactt cggcgttgtt tgccttagat ttcttcagga tggcgaccag ttcgttctgg
    20221 ttcaccgagt tggccgcgcg gctggcatac atcaaacgca cgagcatggg ggtctcctga
    20281 tcttggtgcg aatcgtcatc tcaggtgctc ttcctgtcga gcagcgacag gaactcccgc
    20341 cgcaggttca cgtccttgag gaaggaccct cgcatcacgc tgttgatcat cttggcctcg
    20401 gtgtccttga ccccgcgcca gtgcatgcag aagtgatcgg cctccatcac cagcgccagg
    20461 ccgtcgggct tgacccggtc ctgcaactcg ttggccagca tcgtcacggc ctcttcctgg
    20521 atctgcgggc ggctcatgat ccagtcgcag atgcgcgcgt acttcgacag tccgatgagg
    20581 ttcgagtgct cgttcggcag gatgccgatc cagacacgac ccatgatcgg acacaggtga
    20641 tgcgagcacg cgctgcgaac cgtgatcggg ccgacgatca tcaactcgtt gagacgcgag
    20701 aaattcggga attcggtcac cgcaggcatc ggtgtgtagc gtcccgcgaa gacttccgtc
    20761 aggaacatct tcgcgacacg cttggcggtg tcgtgggtgt tgtgatcgct ttcggtatcg
    20821 atcaccagcg cctgcaacac ctcctgcatc ttggcctcca cctcggcctg cagttcctcc
    20881 atctcgccac cctcgatgaa ggccgagatg ttgtcgttgg cgtggtacct gcaattggca
    20941 ccgaccaggc gataacggat gcgctcggac gcgctcaggc gcgccagttg ctcttcgctc
    21001 ttgaagatcg cagggctgga tttggcaggc atggtcggcg ttccttgctt catgcgatcg
    21061 agcgtaaccc agctcggtcg ccgggtggga cgggggatgc gatgcccgca cgaagcaccc
    21121 ggccacccaa gctcgtagac tccgcgcggt gaaaagccct gcccacatca cagcttcggg
    21181 ggtcactgcc gacaagccga cactggcccg cctgctcgac cacacggccg atgcgatcga
    21241 ggcggtgcgc gccgggcgct cgctgaccgg ggctctggcg cgctgcccgg ctgaggtcag
    21301 gccaggcgtc catgccctca gctcccatgt gctgcggcgg ctgggacagg ccctcgccgc
    21361 gcgggagtgg ctggtgccca aggcgcctcc gaagggcgtc gatgcgctgc tgctgagcgc
    21421 cttggcactg tcctggcccg acggcgaagc accgtacgcc gatcacacgc tggtcgacca
    21481 tgcggtcact gctgcgcgcc ggcgccacgc agccagtgcc gccttcgtca atgccgtgct
    21541 gcggcgcttc ctgcgagagc agccggccgt tctcgaggcc gcacagcgaa cgttgcccgg
    21601 gaagtacaac caccccgact ggtgggtgga acaactgcaa tccgactggc ctgagcactg
    21661 gcaggccctg ctggcagcca acgaccgaca tccgccgatg acgctgcgcg tcaatacccg
    21721 gcgcgtcagc gtctccgcct acctggcaag actgagtgtc gcgggcctgc cgggtcggcc
    21781 cataggtaca cagggcgtga tgctcaacac cccatgcccg gtgagccgcc tgccgggctt
    21841 cgccgacggc gatgtatcgg tgcaggatgc ggcggcccaa ctcgccgcac ccctgctgct
    21901 tgcgggggcc gcgctgccac cgggcgcccg cgtgctcgat gcctgcgctg cgcccggcgg
    21961 caagacggcc cacctgctgg agctggccga cctggacgtg ctggcgctcg accacgatgc
    22021 ggagcgcttg acccgggttg gcgagacgct ggaccggctg cacttgcacg ctcggatgca
    22081 ggccggcgat gccagccagc cttccacatg gtgggacggc aagcccttcg acgcgatcct
    22141 gctcgacgcg ccctgcaccg cctcgggcat cgtgcgacgt cacccggacg tgcgctggct
    22201 gcggcgaccc agcgacgtgc ctgcactggc ggccacccag gccaggctgc tcgatgcgct
    22261 gtggccgctg ctgagtccag gggggcgcct gctctactcc acctgttcgg tattcaaggc
    22321 cgagggccag gaccagatcg atgctttttt gcaacgccag agcgatgcaa gtcgggtcgt
    22381 cgaccctcca tccccgggcc acctgctgcc actgcccgac aatcaaccac ctgctgaaaa
    22441 cccgggatca gctgcccggg ccgacggctt tttctacgcc ctgctgcaaa aaaaccaagt
    22501 ctgagcccct ctgtgcaccg ctgcgcgacg gattgctgcc cctcgaggcc cggtgcgttg
    22561 ttcgcccggt tcctgtcgtt gtgggcgacg tgcctgctgg cactgacgct ctggatcgga
    22621 tctctccccg cgtgggccga gacgccggca cccgaactga gccagttcgc cgtgtcgcgc
    22681 aacgaggagg gactgctcct cgacttcgca gtccgattcg acctgccgca cagcgtcgag
    22741 gatgccctcc tcaagggcgt gccgcttcat ttcgtcgccg acgcctcgct gttccgggag
    22801 cgctggtact ggcgcgacca gagagtgagc cgcgtgacac gcacctggcg cctcgcctac
    22861 cagccgctga cacgaaccta ccgggtgagc ttcggcggct tgaaccagag tttcgacagc
    22921 ctcgccgacg ccgtggcctc cgtgcgtcgc atggggcgct ggaagctcac cgagccgggt
    22981 cagatcgaag agggcgagca gtactacgtc gagttcagct acaagctcga caccaccctt
    23041 ttgccgcgcc cgatgcagat cggcatcggc gggcagcccg actggaccct gcagctcgaa
    23101 aagacccagc gcgtcaccac gccctagtgc cgatcgcatg agcagaacgg gttcccccct
    23161 tgcacgctcc cgccgtgcgg gaccgcgtac cgcgccgcgc tggggccacc tgtgaccaag
    23221 gccaaccgct gggcctggat cgtttcgctg gtggcggcca cgggtgccgg tctggtgctg
    23281 accttcctgc tgtcgatcgc caccaacaac cgcacgttct acgagcgcca ctacacatgg
    23341 ctcttctggg tcaacatggt ggttgccggc ctgctggttc tggtcatcgg catcgctgcg
    23401 gtgcgcctgt tcgtgcgcgt gcaacgcggc aagttcggca gccggctgct gctgaagctg
    23461 gccgccatct ttgcgctggt cggggtgctg cccggcgtgc tcatctacac cgtgagctac
    23521 cagttcgtgt cgcgcggcat cgagagctgg ttcgacgtca aggtcgaggg cgcactcgat
    23581 gccggcctgg ccctgggccg gggcacgctg gatgcgcagg tcaacgaact tgccaacaag
    23641 acccagttcg cagccgaacg gctgggtgaa agcgccggcg aagtacagcc tctgtcactg
    23701 gagcgcctgc gcgagcagct ggccgcccag gacgtcgcga tcgtcaacgg catcggtcag
    23761 gtgctgctgg ctgccaatcg atcggcagca ttccagatgc ctgacaggcc cgcgctgtcg
    23821 ctgctgcgca gcgcccgcag cggcagggtg gcggcgcaac tcgagggcct ggacgacgac
    23881 gcctcgggcg ccggcaccgg aacctccagc ggcgggccgg cccgcatccg tgcgctggcc
    23941 atcctgccca gcgcggacat cgctctggcg cagcaggaac gctatctcct ggtcacgaca
    24001 ctgctgccag cggcgctggc caccaatgcg ctggcggtgc aggcggccta ccgcgagtac
    24061 cagcagcgct cgctggcgcg cgaaggtctg cgcaagatgt acatcggcac gctgaccctg
    24121 acgctgatcc ttgcggtggt cggggcgctg ctgctggcgg tgacgctttc caaccagctg
    24181 gcacggccac tgctgctgct ggccgagggc gtgcgccagg tggccaaggg cgacctggga
    24241 gtcaagcagg tcttcgattc gcgcgacgaa ctgggcgggc tgacgcgatc gttcgcggac
    24301 atgaccgagc agctttcaga ggcgcgctca ctggtgcagc gcagcgtgtc gcaggtcgag
    24361 ggcgctcgcg ccaacctgca gaccatcctg gacaacctga ccgccggcgt gatcgtgttc
    24421 gaccgcgacg gcctcatcga taccgtgaac ccgggcgcga cgcgcatcct gaagctgccg
    24481 ctgtctgcct accgagggcg ccggctcgat gaggtggccg gcctcgacgg attcgccgct
    24541 tcggtgtggc agcgattcga actgcatgcc gacagcctcg aagctggcga acgcggccac
    24601 tggcaggagg ccttcgaact gcagaccgct ccgaatcgcg actcgctgac gctgctggta
    24661 cgtggagcag ccatgcccca gggcgcgcgc ctgatggtgt tcgacgacat caccgaggtg
    24721 gtgtccgccc agcgcatcga ggcctggagt gaagtcgcac gccgcctcgc ccacgagatc
    24781 aagaacccgc tgacgccgat ccagctgtcg gccgagcgtc tgcagcacaa gctgtcggcc
    24841 aagctcgagg gcgccgacca ggccatgctg gtgcgctccg ttggcaccat cgtgacccag
    24901 gtacaggcga tgaagacgct ggtgaacgaa ttccgcgact acgcgcgcct gccggcggca
    24961 cagctcaagc cgctggacct caacgccctc gtcaacgagg tcctcacgct ttacgcaacc
    25021 gcacaggagg ccggcacgct gggcttcgtg ccggcacacg acctgccgct gatcatcgga
    25081 gacccgaccc agctgcggca agtcatccac aacctggtgc aaaacgccct ggacgcggcc
    25141 accgatccag ccaactcgca cacgccgccg caggtgcgcg tgcgaaccga agcagggcgc
    25201 actgatcaag gcgcggtgcg agtcgtgagg ctgcacgtca tcgacaacgg tcccggcttt
    25261 ccggacaagg tgctcaagcg cgccttcgaa ccctacgtca ccaccaagtc caaaggcacc
    25321 gggctgggtc tggcggtcgt caagaaaatc gcagatgagc acggcgcgcg cgtgcgcatc
    25381 agcaacctca tatcaagcga aaccgagggt ggcgcgggtg aggacgccat tcatggcgcg
    25441 caagtttcgt tatcattttc gaaattcgcg ccgggagacg cgcacactgc ggcagcggcg
    25501 agcgattccg cccccgcaca ctgagggttc atggccacca ttcttgttgt tgacgacgag
    25561 ctcggcatcc gggcgctgtt gtcagagata ttgaccgacg aaggtcactc catcgagctc
    25621 gcggaaaacg ccgcgcaagc ccgggcagcg cgtgaacgcg gacagcctga tctcgttctt
    25681 ctcgacatct ggatgcctga cgtggatggc atcacgctgc tgaaggaatg gaacgcaaac
    25741 gccctgctga cgatgccggt catcatgatg agcggtcacg gcacgatcga cacagccgtc
    25801 gaagcgacga agaacggtgc aatggctttc cttgaaaagc cgatcacgct gcaaaagctg
    25861 ctgcgagccg tcgaacaggg tttggtgaag ccggctgcgg cggcgcgcgc cgcagccaat
    25921 gtgacagcgc tgcgaggcga cctggcacat gcctcggtga ccactcactc cagccacgtg
    25981 gcgctggcca gtctgtcgat gaccgcaggc ccgcaaccgg agcaaagttt cgatctcgac
    26041 cgcccattgc gcgaggctcg cgatgccttc gagaagagct acttcgagtt ccacctggcc
    26101 caggagaacg gcagcatgac gcgcgtcgcc gagaagaccg gacttgaacg cacccacctg
    26161 taccgcaagc tcaagcagct tggcgttgac ctgtcccgca gcaagcgcgg cagcctctga
    26221 agtccatcgc ccgcggcgct gatatagtta tgggctcggc ctggtagctc agttggtaga
    26281 gcagcggatt gaaaatccgc gtgtcggtgg ttcgattccg ccccaggcca ccagttagat
    26341 cgaagggtta gctcattcag agctgaccct ttttgttttg gcgcagacgc cgtgcggccg
    26401 cagtcagtgt cggtgtaggc agtcgaaaca tcattcttga cgatcgtcaa tgcctcacgt
    26461 ggtccagcgg atcaaagtga cagtcgacga tagtgaaacg ggttgcaggt cggcatctcg
    26521 aatccgacgt cgaccgcgaa gacacgggcc ctttagctgg gcagccctct gaatctggga
    26581 gtattcatga ccacgcacct tgcacgcgat accagagaca agctcgcgaa gatctatgcc
    26641 gccggcattg ccgtcttcat cgggattttt ggcttcgtga ctgccttcgc ggtgttcgtg
    26701 tcgcacctga agtagccggt ccagcgggcg cgagcgcttg tcgcaaaagg cttgatcgga
    26761 cgagagccgc gcccctgatt caccggcttg gcccgaagac ccgatcggtc attcgcgttt
    26821 gcggtcaccg gtggtcagga gattccgagt gagaactgct ccagcaggcg ctcggtccat
    26881 tcctcgacat cgttgtcgct gagcatcagt gcgcctgcgg cgcagtctcc gcccttgttc
    26941 cactcggcgc tgctgttgag tcccgagaag cgctgaatgg ccacctcggc caccaacccc
    27001 atcgcgacca gccgggtcac cgcatccgga attcgtgggt cctggaatat cccgtagtcg
    27061 tggtgtgacc ggatcgcctg acagacgatc tgtgaaacgc tccaggtgcg ccccatcagg
    27121 ccaccaatca gtgcgtgatc ggtctggtgg tgcgtctgct cgacttcggt gaaccgccgt
    27181 tcgggctcgt cgttgcatga cttcagcgtc gcgccgtaat ccgggaagcg gttgatcagc
    27241 aggggaatgc cgacgtcgca gaacagcccg aacgactgcg cgatgtcggc gtcgacaccg
    27301 cccagacccc gcgcgaggca cgccatggcg tgggaccgct tggccgagac atcccagaag
    27361 cgggtcagtt gaggtccgtc ggttcgaagc accttgcgca gcatcagccc ggtgacgacc
    27421 acggtcacct gcttgagccc gatcatggca atcgcctgat cgaccgtctc gcagtggcga
    27481 cgcaacccat aggcgggcga attaacgata cgcagtaccg ctgtcgtcaa cgcgacatcg
    27541 ccagcgacca gttgggcgac gcgccgcagg tcgggctctt cccgaccgat ctccttttgc
    27601 agctccgcca gcgtggctgg ccgcggcgga atcccgatgt tcttgacgag ggcatctatt
    27661 tcgctgtcgc tcatccccat caagtctgcg ggggccgaaa ctgctgcgct catggtgaga
    27721 actccttagc tggcgttatc ggcatgaaca gccgattcca tgagttcgaa ctcgaccggc
    27781 atcagatgcc tacttgggca tgaacatggt ccctgccaac actccgtcga gtcgtttcaa
    27841 gacttgctga ccgacccctg ccaaaccgac ggccagtcat gggcccacgg cgcggtcact
    27901 gggtgaattc ccccagtcgt tgggcctccg caccccaaat acacgccgca tgcgcacgag
    27961 cagagaactc aagtcgttga tttgaaaagg tttcttgctt tgccaaacac aggcacgggt
    28021 cctgcctggt tggatctcat cttctcgagg aaaacatcat gcaaaccaaa acatccgcct
    28081 tccgactgct ggcgccgctg gccttcgcac tgtgcatcgc aagcgcatca gcgccggcag
    28141 cagcgcaatc gacggtgctg acgttcaacg acctggcgct cgtgagcccg gccacggtcg
    28201 gtgcctcgat gcccgatggg tacggcggct tcgactgggg cggcaggtat tactacatgg
    28261 cccccacggc agcttcgggt aacacgacct acgtggcgat gggctctggt gcatccacct
    28321 ggatccgccg caccgacagc gctgcgttct acttcgacgg agccgacttc tggagccggc
    28381 gtggtctgga tgccaacggc gatttctact ttgtgctctc cctcaagggc aagacggtct
    28441 acaacggtct cacggcgaag aagggggggc gcatgctctt caccggcgct ccgaccctgc
    28501 tgaagcccga ttacgccggg ccaatcgact tgatcgccat cgcgttcagc ggcaacggga
    28561 aagactggaa ccacctcgcg ttcgacaact tccgcttccg cgccatcgca ccctaagctc
    28621 accgcagccc ggggaccggc cccccgggct cgcacgagtg cggcagtgac gaccggtgtc
    28681 aacgcacttt atccagagca gccggcttca cggacactgg accgcgcaca cgggcttcgc
    28741 cgtggcgact tgcagcagag gtgaggcgct gcaaccttca ctaaagtcgg cgtgatgacg
    28801 caccatccac ccgacctgga aagccgccgc caatacagcc ccgcagccga gcgcaacgct
    28861 gcacccatcc tggcgcagct tcagcgcctg ctgccgccgc aaggcagcgc acttgagatc
    28921 gccagcggca ccgggcagca tgcagcgcat ttcgccgccg gtctgcccgg ctggcgctgg
    28981 cagcccaccg atgccgaccc gcacgccctg gcatcgatcg atgcatggtg caacgacttg
    29041 gacaacgtcc gtccggcgct gcacctcgac gtcctggtgg aggcgcgaga ttcgccgaac
    29101 agcgaactcc ccgagaaagt cgatgcgatc tactgcgcca acatgctgca catctcgccc
    29161 tgggaaacct gtgctgcatt gatgctgctg gcctcacggc atttggctcc acgcggcatg
    29221 ctgctgctct acggcccgta ccgggaggac ccggctccca ctgcgccgag caacctggcg
    29281 ttcgatgccg acctgcgctc ccgcgatccg gcgtggggtt tgcgccgact ggccgacgtg
    29341 ctgcaacagg cgaatgcggt cgagttgcga ttgcgcgagc gcgtggcgat gcccgccaac
    29401 aacctgctgc tggtgctgga gcggtcgctc ggtccgacga aggactcggc gtgggcttga
    29461 agtacctgac ggggtatgcg cccgaggtgc tgaaccgcgt gagcgaactg atcgcgcagg
    29521 gtcggctggg cgaaagcctt cggcaacgct atcccgagcc gcacgccgtg cgcaccgatc
    29581 ctgcgctgta cgactacgtg atggagctga agaaccgctt catgcgcagc gcgcctcccg
    29641 tggccaaggt tgcctacgac aaccgcttgc agctgctgca caacgcgctg ggcacgcact
    29701 cggcggtctc gcgcgtgcag ggcggacggc tcaaggccaa gcgcgagatc cgggtggcca
    29761 gcctgttcaa ggagacaccc ggcgcattcc tgaagatgat cgtcgtgcac gagctggcgc
    29821 atctgaagga gcgcgaccac gacaagccgt tctatgcgct gtgcacgcac atggagccgg
    29881 cctatcacca gctcgagttc gatcttcgac tctggctcac ggctcgcgag ctagacggat
    29941 ccaggccggg ctgacgggaa ttcgatcagc ccggccggcg aaattcattc gcgatccacg
    30001 catcggcgct gccagagctc aatcgaaagc ccgccgagac acgcacctgc gccagcagct
    30061 ggccgccggc atcggcagct gcaagcaccg catgcgggtc atcctcgtcg ggcacgatgt
    30121 cgagggtgac cgtgatgcgc ccggagtccg cctgaacgac cagactcttg tgcagttgcg
    30181 catgcaactg ctgttcgtgt cgtcgcagca cctctgatgc ggtcttcacc agcgtgttga
    30241 aagccgtgtt gtcgagcggc ttggggttct tcttgtcgcg ccccatcgtc caggggccga
    30301 ccagtgcggg ttcgacctcg ccctgcttga ccatctccac cgcccatccc tcgtcatcct
    30361 cgttcttgat gacacgagcc gtccagccgc tgtcgcgcca gagacgtggt tcatgcacgg
    30421 tggagtcttc gcctgcgggt tcggtcatgt tgtacggtgt cgtctggcgg ctggactggg
    30481 ggatggggac ccgtcgaggg ttatcggggg ttgtcgaagg ggattgatac ggcgcaaggt
    30541 tcgtggaagc agatggtgtt acacctactc actgatcgtc gaggccacct gcggccatcc
    30601 tgctgcaggc cgtcaccgcg aaaagaagga tacgtgatgc atgttcgctg cctgctcttg
    30661 accactgggc tggcactggt tgtcgcggcc ctgccggctg cggcagaaac gcgtgcaccg
    30721 gcacccgcag cgcgcgcatc ggcgcctgtc gcggtcaagc cgggtccacg gctcatgtca
    30781 cctgccgaga tgaaccagaa cgcgtcgcct cccggcgaat tgcggcccga gaacgccgtg
    30841 acgccacaga tcgtcattcc gctgcgcaac cagtcggctg tcccgccgaa gcccgacaag
    30901 cgcaacctga agccagccgc gaccggtggc gtcaacgacg ccgccgcccg ctgcgacgcc
    30961 ctgacggacg cagccgcacg ggcggcctgc cgtgaaagcg ccaagcgctg aatcggccgc
    31021 gcaagagagg atcgaggagc gccgtccccg tgcttgacat gcatccagca gcccgccaac
    31081 ccgggcccga accctcgttt cgctgtcgcg gcacctgcag cctgcgccga tgagcaccgc
    31141 ttcgcttcct gaacatcctt attcggcgct cgcctccggg accgcattgc gcgtgctcga
    31201 tgagccggag cagacggcgc agttcacccg ctgggc
//
LOCUS       DAOORV010000024        31156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1681_length_31156_cov_7.182984, whole genome shotgun sequence.
ACCESSION   DAOORV010000024 DAOORV010000000
VERSION     DAOORV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 31156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31156
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_31156_cov_7.182984"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1429)
                     /locus_tag="PKV56_05135"
     CDS             complement(284..1429)
                     /locus_tag="PKV56_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX67230.1"
                     /translation="MTNISTSKKALQRTLVSAALLSLVAGAAHAQSSVTLYGRVDLGY
                     QYSDKSRRADGKDGGSVSELANGGIRPSIFGFKGSEDLGGGLKAFFNLESHFDAGTGQ
                     TTSTFFRRQANVGLSSADFGTVTLGRQYTPAVLALLATDPRAIKEQFSSLYTFALNQA
                     GGGSNGNDLGIFAANAISYNKSFGPVNVGLLYGFGEQADTMKAGNFISAGVVYTGPIT
                     VSAYYNEVKNSATSGAAKDAKSEQYGVGVAVPFANFGLPALTAKAQYINAKQDALNDA
                     IGAQRSDTDHFSVGVDYAWNATNVASISYYHSDNDKISKDKTEMFVLSNDFSLSKRTV
                     IYIQTAIVDSDEGSTYFTQALLGPKGQLSPVLDKTNVIFGVGISHSF"
     gene            complement(1829..2620)
                     /locus_tag="PKV56_05140"
     CDS             complement(1829..2620)
                     /locus_tag="PKV56_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOX67231.1"
                     /translation="MERMQRMRWFAWAVAALVAALGTAGSVLAQDASASAAASPQASV
                     LGAWKIVQARTEPILDQRYARLELGTDGRLGGHTSCNSMSAPYTLDGSKIRVGAIVTS
                     RMACSQAKLEQEDRILTALESAVTAIVRGDGLLEIRDSEGRGLLRAIRFEADTNAPDQ
                     TPSPAQPGKWPVVGRQGIIQVVIVPSELARERASYDRQIASICLPDQSCFINFYTNAS
                     GAPLVLPLPESISSEATAIFRRSIKQAGEFFRWSCRLGMADADCF"
     gene            complement(2636..3025)
                     /locus_tag="PKV56_05145"
     CDS             complement(2636..3025)
                     /locus_tag="PKV56_05145"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOX67232.1"
                     /translation="MSSWRKKTVDEVFTPEEVAARLQQELPTWSYEDGWIRRKYKTSG
                     WKGTLMVVNTVGHLAEAAWHHPDLAVSYAFVIVKLVNHSAKGITEKDFQLAKKIEEVV
                     LWQPGQEDGSELEGTPDDPRFKYIKYD"
     gene            3133..4080
                     /locus_tag="PKV56_05150"
     CDS             3133..4080
                     /locus_tag="PKV56_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOX67233.1"
                     /translation="MASSLLALIDDIATVLDDVAILTKVAAKKTAGVLGDDLALNAQQ
                     VAGVNADRELPVVWAVARGSMLNKAILVPAALAISAFASWAVTPLLMVGGLFLCFEGF
                     EKLAHKFMHDAKDDAAHEAEVLKALTDPSIDMVAFEKDKIKGAVRTDFILSAEIIAIT
                     LGTVATSPFVTQVTVLVGIAIVMTIGVYGVVAGIVKLDDLGLYLTRRSGAGAAFQRRL
                     GAAIVKAAPYLMRTLTVLGTAAMFLVGGGILTHGIPALHHAIEMAAAKVSGIPGIGGL
                     FGAVTPTLLDGLSGVVAGALALAGVSLAVRLFKRPATAA"
     gene            complement(4131..4535)
                     /locus_tag="PKV56_05155"
     CDS             complement(4131..4535)
                     /locus_tag="PKV56_05155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX67234.1"
                     /translation="MLAISTSHCAWAQTDAPAKEAGATTTSSGLIYRSLTEGTGASPG
                     ATDTVKVHYRGTLPDGTEFDSSYKRGQPASFPLNRVIPCWTEGVQRMKVGGKARLTCP
                     PQIAYGARGAGNVIPPNATLFFDIELIDVAAR"
     gene            4742..5722
                     /locus_tag="PKV56_05160"
     CDS             4742..5722
                     /locus_tag="PKV56_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOX67235.1"
                     /translation="MKLLLAPMEGLLDHTLRDILTRVGGVDRCVSEFIRVSGTLLPDR
                     AFLRVVPELNQGGRTRAGIPVRAQLLGSDPNCLAENAARLAALGPHGIDLNFGCPAKI
                     VNRHGGGAALLDDPELIAAIVSAVRRAVPRTMPVSAKMRLGNLDGSRAVECAQAIAGA
                     GADELVVHARTKADGYRPPAYWERVAEVRAAVSIPVAANGEIWTVADALRCRELSGCD
                     TLMLGRGMVVDPGLALAIVAATRQAPLSDAAARGMPWESLLPLVTEFWSLVLARRDIA
                     DRHRAGRLKQWLNLLRRCHPRAEAIYGAIRTVNDPAQIGAYLAESVRIAA"
     gene            5844..6608
                     /locus_tag="PKV56_05165"
     CDS             5844..6608
                     /locus_tag="PKV56_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67236.1"
                     /translation="MPTSVPPVKTPLGHEELRTRSHGLNQRHRTMLFLVDGRRPLSEV
                     LALALQAGASTTHFEDLVHLGLVELVIDPDEASEVMPSQQGALDSGGASTRSGVLAVE
                     LGPPSSPVEPPPRAPLPRPVVEVLEVDVDAVPPPSRPVPLNADHTGSALSVPQRRALA
                     KASDDELFQQVRELLIDTLRVDAPVFSAITLMRVHRARSSKELINLVWEIERHLGGPS
                     RRRREMSSLHRARELLGMGNTQVSLDAEETGFPDTE"
     gene            6786..7460
                     /locus_tag="PKV56_05170"
     CDS             6786..7460
                     /locus_tag="PKV56_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX67237.1"
                     /translation="MTRTDALNDSSRRRRWLALLVVSLTAFTAIALNAGSGSHLQAID
                     QQASQHLLAHGSSTWTLAMQWLARVHGVAGIAIVGTLWLAWLGLRGETMQLVRLALIV
                     GGGLVLNSVLKRVFQRARPVFGEGVELPLETLATYSFPSGHVSGSVVFYGFVLMTIFS
                     ATQHRAWRSASVVVAGTMVVLIAYNRVYVGAHFVSDVLAAAAEGLAWLAFCALLLERV
                     QTRPSK"
     gene            7476..8405
                     /locus_tag="PKV56_05175"
     CDS             7476..8405
                     /locus_tag="PKV56_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOX67238.1"
                     /translation="MRDLIPVIVNSAARSGDSRAQIAALPGLFESAGLRIRLIDLCRG
                     ADSDAEIDAALAARPSMVVAGGGDGTLSAIAARLVDTGVPLGVLPLGTLNHFAKDLGI
                     PLAVDAAVQVIAAGHTADVDVGEVNGRIFINNSGLGLYADIVRHRDRQQRTLGRGKWY
                     ALAWATWSMLRRYPFVHVRLVIDGCSREWRTPFVFVGNNQYAMEGLRIGVRSRLDEGL
                     LCVYVAQQPGRLGLLRLALHALLGRLSQASDFDAMTATELLIETRRRHLHVAADGEVW
                     PMTTPLRYRTRPGALRVIVPAPPSAETSGLDSG"
     gene            complement(8353..9189)
                     /locus_tag="PKV56_05180"
     CDS             complement(8353..9189)
                     /locus_tag="PKV56_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX67239.1"
                     /translation="MTTLLHISDTHFGTEQAPVVEALARLSSDLAPELLVLSGDITQR
                     ATPEQFASARRFVDRLGVPWLAIPGNHDIPLFNGVARLLFPYARFQEAFGEALEPTHD
                     SADLLVLCVKTTRRLRHVEGQVSPAQIERVAARLRSARPGQLRVVVVHQPLHVEGDAE
                     RKHLLRGCETALRQWAGAGADVALGGHIHLPYMRELTPAGAGGASLVVAQAGTALSSR
                     VRRDVPNSVNVIRTGHPGDVGYTIERWDYSAEHSAFQRIESRRFSRNRARTSQPMAAQ
                     AR"
     gene            complement(9238..10155)
                     /locus_tag="PKV56_05185"
     CDS             complement(9238..10155)
                     /locus_tag="PKV56_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOX67240.1"
                     /translation="MKSWILALSVAVMAAGLAPLSVDAKRLGGGSSSGMQRSLPSRSA
                     PDATPAKPAQATPTAPATAGAAPAAAPKRSWMGPIAGLAAGLGIAALMSHLGMGAEFG
                     NILMMLLLAGVAFVAIRVVISRMGAKSSASPMRAPGGMQFAGAGAAAGSGGSGSGWNP
                     QPAPEPVAASPALNQPYLRPPTVPVGFDQAGFERIAKMIFIRMQAANDSADLNDLRQF
                     TTPEMYAAVKLDLQERGSSAQQTDVVRVDAEVLDFSDEADRQVVSVRFHGLIKEEASG
                     PTQPFDEVWHLAKPNDGSQWAIAGIQQTN"
     gene            10367..11797
                     /locus_tag="PKV56_05190"
     CDS             10367..11797
                     /locus_tag="PKV56_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX67241.1"
                     /translation="MTHALIVDDDVDSASTLKALIVGEGLTVAVVHSLRDARRQIALQ
                     QPDIVLLDLQLPDGNGMDLLADRHMLADSEIVLITGHASLETSIQALRLGAADYIVKP
                     INMRQLQGVLSRFMKPAVLKAEVEGLVAQVEPTGHFGHLWGRAPSMLRLYEQITRVAG
                     TSVTVFITGESGSGKEVVAQTVHDLSRRRKQAFLAINCGAISPNLIESEIFGHEKGSF
                     TGADRQHLGFFERASGGTLFLDEITEMPMELQVKLLRVLETGRFMRVGSTQTQETDVR
                     VLAATNRSPEQAVASGKLREDLFYRLNVFPIELPPLRDRLSDVPLLARHFLTAFGAQQ
                     GQVKQFSAAALDRLSRYRWPGNVRELRNAVQRAYVMSAGGLIDEECLPDMAFAVAPAA
                     VAAARVAESPPRDAVPAQMTDDSITLPIGTSLAQCERALILATLRAYHHHKERTAAAL
                     GISLKTLYNRLKEYAGDSTGKAVDPD"
     gene            complement(11813..12472)
                     /locus_tag="PKV56_05195"
     CDS             complement(11813..12472)
                     /locus_tag="PKV56_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX67242.1"
                     /translation="MNEHALRVALTTVAPPYSAASTPRPAVFIDQGGTLVEKVPYNAD
                     PALLRFKPSACEAMAMLASAGLPLVLVSNQSGIGRGYFTRGQFSALQSALERRLRDEA
                     GVELTDFVICPHVPAAGGQPACLCRKPAPGMLVRAARAHRIDLSASWMVGDKLDDVEA
                     GRRAGCRTVLLDDGGETVWRRSPLRIPHARCVDWPSVSRLILADRSGLRAAVMPPAVT
                     S"
     gene            12726..12959
                     /locus_tag="PKV56_05200"
     CDS             12726..12959
                     /locus_tag="PKV56_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67243.1"
                     /translation="MKHIDSTCSTAYSHDCHEPAGDDGFCFDLCLAPLLPAGVEPWLS
                     TRAIFFVDGELPVSGRLAFDTLNARRSPEFVSD"
     regulatory      13098..13192
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            13280..15322
                     /locus_tag="PKV56_05205"
     CDS             13280..15322
                     /locus_tag="PKV56_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX67244.1"
                     /translation="MNKILRTLCGLTLFLGLCASAWSDGHRADRVPHVEKPRFPQLDL
                     PDRHSGGQRAIDGLGTRLSEVANWYGKSPDEFRGMLMKDRRMRLDKRGRVFIVEELDQ
                     PMHATPVSAADTSLLDGTLLPLDQTFLLHSRPGAKRTIYLNFKGATLTGTAWNGTGGS
                     LTALPFDIDGIPYTFSTTELQRIQYIWQRVAEDFAPFDVDVTTEAIAPELITRAGSAD
                     TVFGTTVLITKRTGVYNCSCGGVAYLGVFDDTREFYKPALVFYDALGNGNEKYVAEAV
                     SHEAGHNMGLGHDGTATAGYYQGQGSGATGWAPIMGIGYYQPVVQWSRGEYTGANNVQ
                     DDYAVMQSNGLPLRNDDHGNTLASASVLIGAASGGMTTTSAQGVIERPTDVDLFAFSA
                     GAGTATFKVTPAARSTNLDAWVGLRDAAGNLLANINPVDALNAIVTFNLPVAGTYYLS
                     VQGTGKGDPLTTGYSAYGSLGQYAVSGNFFTPGSQPPTSVISASTLRGVAPLAIAFSG
                     AASRDPDGSLVAYDWTFGDGGSASGATASHTYSAPGSYAAVLRVTDNTGLTASSTVTI
                     TVDAPVVVQTMRVSDIAMSLRVSRTGSAQAGAAVKVVGSNGLPLVGATVAGAWSGTFA
                     RSGSVVTDSTGVARFSSPSTKSTGASFIFTVKGVSGVTGYTYAPLTNTETSDSIAR"
     gene            complement(15407..16351)
                     /locus_tag="PKV56_05210"
     CDS             complement(15407..16351)
                     /locus_tag="PKV56_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67245.1"
                     /translation="MNASPRSRMTLLMLSVLLPGLLAVAWVTANTWRWERHAPPADWA
                     ASATLQVAIWALCLLGVALAGLWWVATRPVVEPSESPLQKPAREPLPDRVDEASTTDR
                     NRIEALGRLTGGVVHDFNNLLGVISNSAHLIERQVQAAELQAPVAATLRAVEAGSRLT
                     QHLMRFSDRQPSPPHAVDLSSMLSESDEFLRAIAGRRITLSVAVAATTRHAMVDANGL
                     ELALTTLTLDARDAMPSGGRLRLHACNAATEDTYDLAAGDYVLITVGDDGTADGESTG
                     PTPSLQQVHAFCVRSGGTSRRASTPGLGTTVSILLPAC"
     gene            complement(16450..17400)
                     /locus_tag="PKV56_05215"
     CDS             complement(16450..17400)
                     /locus_tag="PKV56_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX67246.1"
                     /translation="MKTIRTLLTASIALALAACQTTAPQNPDLVAARTTLSQARSNPA
                     VARSGAVELERALQAMNAAETAWANDRNVDETRHLAYIARQRAEIALAVGSQAEADQR
                     IQKAGAERERVQLDARSREADVATANARVAQADARTAKASAVAAQGAAQTSRNEAVLS
                     REQADAARLLAVQQSERAAGLEQDLQELKGRSTDRGMVVTLGDVLFDTGRATLQPGAR
                     RSVEQLVQVLGRHPERRVLIEGFTDSVGGEEMNLQLSQRRAETFQRQMTNAGIAVERI
                     EVRAWGEANPVADNGTPAGRQQNRRVEVLFSDAQGRIAAR"
     gene            complement(17427..17828)
                     /locus_tag="PKV56_05220"
     CDS             complement(17427..17828)
                     /locus_tag="PKV56_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOX67247.1"
                     /translation="MKTLILASLMASGALAVTGCAHTPSPAPSMAATAASVEAARSAG
                     APEMAAAELNAARSKLDRARALAAEGDERGAIRLAEEADVDAQLARARAGSERSRRAV
                     TELEAGLQTLRDELNRAAAKQPVSAPATAPQ"
     gene            complement(18163..18414)
                     /locus_tag="PKV56_05225"
     CDS             complement(18163..18414)
                     /locus_tag="PKV56_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67248.1"
                     /translation="MASSRTKTYGTNGTFVGIPGGHPITSLLGTIIVGVAAGYVGRSQ
                     GSEAAMLILGTLGGIAGYLGGDAIGNSIQEFYRSEERRG"
     gene            complement(18509..20146)
                     /locus_tag="PKV56_05230"
     CDS             complement(18509..20146)
                     /locus_tag="PKV56_05230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX67249.1"
                     /translation="MNNDPGGEATDGPARELARLQSRIEDARAELTSMQHKLAEVMNR
                     LGEGEGDRLREANENLIVAVLQSRADAEAAAQGMLRAARLAELDPLTQLPNRVLLLDR
                     FEQAIASARRSGKQVALLFIDLDDFKQVNDTLGHAIGDEVLQLAARCLVASVRAADTV
                     SRHGGDEFLILLDGVSQAQDAAAVADKVIAALGAPALLGSHVMRMKASIGISLYPSDG
                     DNASALIALADGAMYRAKRHGMGSFVFHDGQSLGQPLVQTPPLASLRSPLSHRESARK
                     EFEQRQAQLQEANQQLLLSALDARELQAAAELALRRQTQFMSRAVHELSNPLMSMRSA
                     SAVIGRTGGDALVLPKMASIIERQLVQMSRLVSDLLDVSRVSSGKLRLERRAVEMTGL
                     IDEVVAACRPAIDTRLQQFSVQAHLGELPVYGDPLRLSQVLRNLLDNASKYTPQGGYI
                     RLSVVRIDESLVITVSDNGIGIDANALSHIFEPFVQDSAATVFDGSGLGLGLTVVREL
                     VKAHGGEVEAFSAGPGCGSEFRVTLPLVETKALPDGD"
     gene            complement(20181..21689)
                     /locus_tag="PKV56_05235"
     CDS             complement(20181..21689)
                     /locus_tag="PKV56_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOX67250.1"
                     /translation="MNKVTINRLATGVPGLDEVLGGGLPELSFNLIAGPPGCGKTTLA
                     HQMMFALAKPEFRAIYFTVLGEPPLKMLRYQQQFEFFDSEAVNRSIRFINLSDDTLTG
                     DLDRVLARIVAEVEAYGPALVFVDSFRSVVLSAAPPNNPEKSLQQFVQQLGMLMTSWQ
                     ATTFLIGEYFVETDANPVFTVADGLIWLRQSVQRNSMVRKMEVMKMRGQPTLPGLHTF
                     RIGNAGIEVFAPADQMAQALPHLNGLTPSGNTSPEARLLTGVPGLDDMLGGGLPRGYS
                     LLVAGPSGSGKSILAAAFLAEGNRLGETGVIAAFEQRPNRTRNPLVADLIASGRVALV
                     DSHAPDLSIDEIVMLLTREIRRLGATRVVIDSLSGFELAVAPTFRDDFRESLSRLVTA
                     LANAGVTVLMTSELEDRYTDLRFSPYGTAFLTDAIIVQRYIEVDSRLRRVMAVVKVRA
                     SAHSDELREFTIGDEGIRIGQTLGDQEGLLGGRPTRKRHAIDGAAMPTGRAD"
     gene            complement(21686..22120)
                     /locus_tag="PKV56_05240"
     CDS             complement(21686..22120)
                     /locus_tag="PKV56_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67251.1"
                     /translation="METPERPLITAPLANRVRADAEASQVAEAVEAIWLEIDAALTPI
                     VGQRGLVALYKRSLYVAATVHPWLDGLQEGVPPAMDLARIRTAFARQTGAAAVAGGCA
                     MFHALHDLLESMVGPSLTERLLRPVWALSPHGPAAHAQDTTS"
     gene            22413..23882
                     /gene="rpoN"
                     /locus_tag="PKV56_05245"
     CDS             22413..23882
                     /gene="rpoN"
                     /locus_tag="PKV56_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOX67252.1"
                     /translation="MTNLGFRADHQQTQRQSLSTRFQQAVRLLQMSSLDFAASVRDVL
                     GKNPFLEVEEGDGEADQDAAADDPGDGADSTADSDGLSAELAMPREGLDEVGESDNDR
                     DLWRADSGVSPRSADDGELSAMNQMAVETSLNAHLHGQLNLLPLSLRELLLARVVVES
                     LDDDGYLRTSLDTLVGLEAMSPAATLTEMRAALRCVQSLDPCGVAACSVAECLLLQLP
                     SIDCAQTRALAERIVGDHVGALAARDTHGLARALGESPVRIQAVCDRIRHLDPRPGWR
                     FSAERVVYVVPDVIVRKVRGVWSAQLNPAIVPRVRLNQVYAELFKRHRTADNAELADH
                     LQEARWTVRNVEQRFSTILDVAEAIVKRQQHFLDFGPMAMKPMGLREIADEVGVHEST
                     VSRVTNNKYMSTPCGVFEMTHFFSRAMVSTNGSACSGTAIRGLVKDLIDAESPAAPLS
                     DVEITRQLAEQGLVVARRTVTKYRQLLRIEAVDRRRRLS"
     gene            23997..24596
                     /locus_tag="PKV56_05250"
     CDS             23997..24596
                     /locus_tag="PKV56_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67253.1"
                     /translation="MSIDMELVDSWPESVRVEVQVHYASGPCDARNFSVEPCPSQHAV
                     RLFNLRLDCEPLEMHRIGWVDLPGRALYQRCLSVLALHWFGSIDMVPRVRGVPLWRSL
                     SAELTMPSAHVNRVKFDIQAVNDTSATLRYQFRRDQPLVNGTVMLDPLDADPLRALLR
                     VGLGRVKPMAVPRKAASRAPARSLPSHSAASGHAASQQA"
     gene            24615..24803
                     /locus_tag="PKV56_05255"
     CDS             24615..24803
                     /locus_tag="PKV56_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67254.1"
                     /translation="MSDNAESALPDLIRGHPLLSQLRSSLQSEAAAHEGPDSSGWDPV
                     ASALALSGMLPEPPAISG"
     gene            complement(24793..26829)
                     /locus_tag="PKV56_05260"
     CDS             complement(24793..26829)
                     /locus_tag="PKV56_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67255.1"
                     /translation="MSAVPSPVFLDHAGECARLLRAEDRCGLGPAAGWPAELRLLTGL
                     MLGASEAMFVAWGPQRCFIYNDAYAPILGARHPAALGQPMQHVWSDIWADIGPLADSV
                     YAGHSVQMDDIALSIPRGGRVEEAHFSFGYHPVRDDQGALSGLFCVCRETTVQRREQA
                     ERDQATARLVQSEAARQISEERFQAAVRAVGLMWTQNAQGEMDGEQFGWAALTGQTPA
                     QYRGDGWSSAIHPDDLQPTLIAWKQAVKQQRHFEWEHRLRSAAGQWRRYAVCAEPVFA
                     ADGHLREWVGVHIDVTEARRTAESLREADRRKDEFLAILSHELRNPLAPIRSAASVLA
                     NPALTAEELAVSRDVINRQIRHMAWLLDDLLDISRISAGRLELEKQPHRLQAIVEAAV
                     ENARVLIDGRRHTLEVDLQPGCVAHVDEVRVTQVLANLLLNAARYTPPGGQIRLAARA
                     DEHELLIEVQDNGIGITPEQLPYVFDMFSQLRSASDRTQGGLGIGLALSRGLVELHGG
                     HLDASSAGLGGGATFRVHLPPAAFDSRPASLDEAPAVLAAPVVTPLRILIADDNRDAA
                     DSLGLLLGLEHHEVHVAFDGARALALAVQVHPQVAVLDIGMPDLDGYEVARRLRLQPG
                     GRDMLLLALTGWGQQEDQLKALAAGFDRHFTKPIDPKTLIDCMNAWYSKQPVSR"
     gene            26950..27735
                     /locus_tag="PKV56_05265"
     CDS             26950..27735
                     /locus_tag="PKV56_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX67256.1"
                     /translation="MKCNCKTLSGFPSSAARLWSVPFDALQSARKNRLLAALPLAEWQ
                     RWLPHLEWVDMPPGRVLHESGHALDHVYFPTTAIVSLLNVTQDGASTEIAVVGNEGVV
                     GTALFMGSDSTCSRAVVQSGGFGFRLKARPLRDEFERSGVVMHLLLRYTQALMTQMAQ
                     LAVCNRHHSIAQQLCRCLLVHLDRLGGQEIVMTHESIASMLGVRREGVTETALKLAAA
                     GLIRYARGRITVLDRAGLEQRACECYGVVSNEYDRLLSERVAA"
     gene            27974..29203
                     /gene="tolB"
                     /locus_tag="PKV56_05270"
     CDS             27974..29203
                     /gene="tolB"
                     /locus_tag="PKV56_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOX67257.1"
                     /translation="MAAAPVALAQFRVEITGVGAAQVPIAIARFRDEDRQSQPLAAIV
                     RADLERSGLFRGVDAPGVLDELRTPAMAEWRTRAADALVGGSVTKLADGRFDVRFKLW
                     DVVKGSELGGQSTVVDQADLRLAAHRIADFIHEKLTGQKGVFSTRIAYVTKVGNRFTL
                     RIADADGEGGQVALNSTEPIISPAWSPDGRELAYVSFESQKAVVYVQEVASGKRRAVA
                     NFRGSNSAPAWSPDGQTLAVTLSRDGGSQLYQMGRNGDNVRRLTQSIGIDTEPVYSPD
                     GRSVYFVSDRGGGPQIYRTSVNGAAVERITFTGSYNISPAISPDGRMLAYISRQGNAF
                     KLHVQDLSGGAPQVLSDTTDDESPSFAPNGRLIIYASRSQGRDVLMTTTLDGKIRARL
                     VSSNVDVREPVWGPFGR"
     gene            29279..29851
                     /gene="pal"
                     /locus_tag="PKV56_05275"
     CDS             29279..29851
                     /gene="pal"
                     /locus_tag="PKV56_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOX67258.1"
                     /translation="MTPFRLRACAHQRLLWTSLCAVALVGCGSNVKLDENAPPVESRS
                     GTAVNPDGSAGASSGGTSQSQVTPVDLTKGAGGDAANAANRIVYFDFDSYVVRDDARP
                     VIDLNAKALAANKAKKMSAEGHTDERGGREYNLALGQKRAEAVVRALTLAGAADGQVE
                     AVSFGKERPAVVGSDEAAYAKNRRVELVSR"
     gene            29862..30665
                     /gene="ybgF"
                     /locus_tag="PKV56_05280"
     CDS             29862..30665
                     /gene="ybgF"
                     /locus_tag="PKV56_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOX67259.1"
                     /translation="MSRPKPLPHRFWFSACLLMALSAPAAHAGLFDDDEARRAILDLR
                     QKLEQGNEAQRARQAELNAQMTDQIGQLRSSLLDLSNQLELMRTENARLRGQVEQLAR
                     EASELQVQMKDLKELMQGLDDRTRRLEPQKASVDGKDFMADAEEIRQYEAAMTLVRSG
                     DFAGAATALAVFRSRYPASGYGQSVLFWLGNAQYGKRDYLEAISSFRGFAGSYPGDLR
                     APEALLAVANCQFELKDVKSARKTLDELFKAYPDSEAAQAGRQRLAALK"
     gene            30708..>31156
                     /locus_tag="PKV56_05285"
     CDS             30708..>31156
                     /locus_tag="PKV56_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOX67260.1"
                     /translation="MTDDADLERRFGGLRRLHGDASYQRLRAARIAVVGLGGVGSWAA
                     EALARSGVAELTLIDLDHVSEGNINRQIQATGQTIGQAKAQALCERLADIHPGCVLRP
                     IEAFVDAENWPALLTHEIDVLVDACDQVRAKATLAAWALATRTAFVTV"
BASE COUNT         4878 a        10256 c        10615 g         5407 t
ORIGIN      
        1 ggctcgaccg ctatcacatg ggtatcggaa cgggcgaccg ctcccgctcc gagctgcggc
       61 ctgccagcga gcccgagccg ctcgggtgtt cgcgcaccac cgacggaccg caggagtggc
      121 ggctggttcg agcgatcttc gtgaactgaa aaaagaaagg accgcatgaa gcggtccttt
      181 tgagttgcag cactgcgcgt gtgcgtggtg ctgcgtggtc ttcttgttcg attccgcctg
      241 cgcactgcgc cgagtcggcc gcagtttcag gttggcgtgt cgatcagaag ctgtggctga
      301 tgccgacgcc gaagatcacg ttggtcttgt ccagcacagg cgacagttga cccttcgggc
      361 cgagcaatgc ctgggtgaaa taggtcgaac cttcgtccga gtcgacgatg gcggtctgga
      421 tgtagatcac cgtccgcttc gacagcgaga agtcgttgct caggacgaac atctcggtct
      481 tgtccttgct gatcttgtcg ttgtcgctgt ggtagtagga gatcgaagca acgttggtcg
      541 cgttccatgc gtagtccacg cccaccgaga agtggtcggt gtcggaacgc tgggcgccga
      601 tcgcgtcgtt cagggcgtcc tgcttggcgt tgatgtactg cgcctttgcg gtcagcgcag
      661 gaagtccgaa gttggcgaac ggaacggcca cgcccacgcc gtactgctcc gacttcgcgt
      721 ccttcgcggc acccgacgtg gccgagttct tgacctcgtt gtagtaggcc gacacggtga
      781 tcggaccggt gtagacgacg cctgcagaga tgaagttacc agccttcatc gtgtcggctt
      841 gctcgccgaa cccatacaga aggccgacat tcaccgggcc gaacgacttg ttgtacgaga
      901 tcgcgttcgc agcaaagatg ccgaggtcgt tgccgttgga tccgccaccg gcctggttca
      961 gcgcgaaggt gtacagcgac gagaactgct ccttgatggc gcgcgggtcg gttgccagca
     1021 gggccagaac cgcaggggtg tactgacggc ccagggtcac ggtaccgaaa tcggcgctcg
     1081 acaggccgac gttggcttgc cgtcggaaga acgtcgaggt cgtctgaccg gtgccggcat
     1141 cgaagtgcga ttcaaggttg aagaacgcct tcaatccgcc gcccaggtct tcgcttccct
     1201 tgaagccgaa gatgctgggg cgaatgccgc cgttggccag ctcggagacg ctgccgccgt
     1261 ccttgccgtc agcacgacgc gacttgtcgc tgtactggta gccgaggtcg acgcgaccgt
     1321 acaaggtcac cgagctttgg gcgtgggccg cgccggcaac gagggacagc agggctgccg
     1381 aaaccagcgt gcgctggagc gctttctttg aggtagagat atttgtcatc tgtttctcca
     1441 tgggttcgaa actatctgag tcccgacgaa gtggcctgct tcttgtgagc cgcctacgac
     1501 gcgtgtgagg gttgcgaaat cagaggtaac tgggaaaatt tcctctcgca cttccatttt
     1561 gagagtgcta tggacatcgc tgcaatcagc atgacactga tccaaactgg tgccagctct
     1621 tgcattgatg cgcttttccg gggctcgggg ctccgtgcag cgtcgcgcag tatagggtta
     1681 gtgcctagcc aatttcacag gccttgttgc tctggtgaca catcggcggt gcacttcata
     1741 tattgaaaat gcactatttt ggtgcaattc cgagggtgag atccatgagt gctgccggac
     1801 agattccggc atgtccaaag gactgggatc agaaacagtc ggcgtcggcc attcccaggc
     1861 gacaactcca gcgaaagaat tcaccggcct gcttgatcga gcgccggaag atcgcggtcg
     1921 cctcgctgga tatggactcg ggcagcggca gaacgagcgg agcacccgaa gcgttggtgt
     1981 agaaattgat gaagcagctc tggtccggca ggcagatgct ggcgatctgg cggtcgtagg
     2041 acgctcgttc gcgtgccagc tccgatggaa cgatgaccac ctggatgatg ccctggcgac
     2101 ccaccacggg ccacttgcca ggctgcgccg gtgacggggt ctgatcggga gcgttggtat
     2161 cggcctcgaa gcggatggcg cgcagcaggc cccggccttc gctgtcccgg atttccagca
     2221 gtccgtcgcc tcgcacgatc gcggtgactg ccgactccag cgccgtcagg atgcgatcct
     2281 cctgctcgag cttcgcctgc gagcacgcca tgcgtgaagt cacgatcgcg ccgacccgaa
     2341 tcttcgatcc gtccagcgtg taaggcgccg acatgctgtt gcagctggtg tgcccgccaa
     2401 gccgaccgtc tgtgccgagt tcgaggcgcg cgtagcgctg gtcgagaatc ggttcggtgc
     2461 gcgcctgcac gatcttccag gcacccagca cgctggcttg cggcgaagcc gcagcagacg
     2521 ctgaggcatc ctgcgcaagc accgaacccg cggtgccgag ggcagcgacg agtgccgcaa
     2581 ccgcccacgc aaaccagcgc atgcgttgca tccgttccat gcgcgcggaa ttccgtcagt
     2641 cgtacttgat gtacttgaag cgcggatcgt ccggcgtgcc ttcgagctcg ctgccgtcct
     2701 cctggcccgg ttgccagagc accacttcct cgatcttctt cgcgagctgg aagtccttct
     2761 cggtgattcc cttggcggag tggttcacca gcttgacgat gacgaacgcg taggacactg
     2821 ccagatcggg gtggtgccag gcggcttcgg ccaggtggcc gacggtgttg accaccatca
     2881 gcgtgccttt ccagccgctg gtcttgtact tgcgccggat ccagccgtcc tcgtaggacc
     2941 aggtcggcag ttcctgctgc aaccgggcag cgacttcttc gggtgtgaac acttcgtcga
     3001 cggtcttctt gcgccatgaa ctcatgggtg ctccttgctg atcacatgcc gggaatgttc
     3061 gcacgacccg acatgaccgc gactccagcg cggctacgat cccacgctgc ttccaccctg
     3121 ggaaagaacc cgatggcctc cagcctgctc gccctgatcg atgacatcgc caccgtgctc
     3181 gacgacgtgg cgatcctgac gaaggtggct gccaagaaga cggcgggcgt gctcggagac
     3241 gatctggcgc tgaatgccca gcaggtggcc ggcgtcaacg ccgatcgcga gctgccggtg
     3301 gtctgggcgg tggccagggg atcgatgctg aacaaggcca tcctggtgcc ggctgcgctg
     3361 gcgatcagtg ccttcgcgtc ctgggccgtg acaccgctgc tgatggtcgg cggtctcttc
     3421 ctctgcttcg agggcttcga gaaactggcg cacaagttca tgcacgacgc gaaggacgat
     3481 gcggcgcatg aggccgaggt gctgaaggcg ctgaccgatc cgtccatcga catggtggct
     3541 ttcgagaagg acaagatcaa gggcgcggtg cgcaccgact tcatcctttc tgccgagatc
     3601 attgccatca cgctgggcac ggtggccacg tcgcccttcg tgacacaggt gacggtgctg
     3661 gtcggcatcg cgatcgtgat gacgatcggg gtctacggtg tggtcgcagg catcgtcaag
     3721 ctcgacgatc tcgggctgta cctgacgcgg cgatcaggag ccggcgcggc attccagcga
     3781 cgattaggag ccgccatcgt gaaggccgcg ccttacctga tgcgcacgct gaccgtgctc
     3841 ggcacggccg cgatgttcct cgtaggcggc ggcatcctga cccacggcat acccgcgctg
     3901 caccatgcga tcgagatggc cgcggcgaag gtgtcaggaa tcccgggaat cggcggcctg
     3961 ttcggcgcgg tgacgccgac cttgctcgac gggctgtccg gtgtggtggc cggcgctctg
     4021 gccctggccg gcgtcagcct cgccgtcagg ctgttcaagc ggccagcgac ggcggcgtga
     4081 tcttcatgcc cgtcgctggc cggcccgagg cgccggatcc ggcgcgtctt tcagcgcgct
     4141 gcgacgtcga tcagctcgat gtcgaagaac agcgtcgcat tcgggggaat cacgttgccc
     4201 gcgccccgcg cgccgtatgc gatctgcggc gggcaggtca gacgtgcctt gccaccgacc
     4261 ttcatgcgct gcacgccttc ggtccagcaa gggatgacgc gattcagcgg aaagctcgca
     4321 ggctgtccgc gcttgtacga gctgtcgaac tcggtcccgt cgggcagcgt gccgcggtag
     4381 tgcaccttca ccgtgtcggt ggcgccgggg ctggcgccgg tgccttccgt gagagaacgg
     4441 tagatcagcc cgctcgaagt ggtcgtggct cccgcttcct tggcgggcgc atccgtttgc
     4501 gcccaggcgc agtgcgaggt ggaaattgcc agcaagccgg cgtagacgat ggccttcaaa
     4561 tcgtttctcc aggaaggtgc attgaggatt tcctgcgcgg tcgattgcga acgtgcggga
     4621 ccgcgatcat cgccgcagcg gcgatccggc gtcctcgaag gcactttgct gcgcttgcgc
     4681 atcagctcaa catagccctc gcaggaacag cggagcccca gccgccccgg tttgctcgtc
     4741 catgaaactg ctgctcgcgc ccatggaggg cctgctcgac cacacgctgc gcgacatcct
     4801 gacgcgggtc ggaggagtcg atcgctgcgt gtccgagttc atccgtgtgt cgggcacgct
     4861 gctgcctgac cgtgctttcc tgcgcgtcgt gcctgaactg aatcagggtg ggcgcacccg
     4921 cgccgggatc ccggtgcgcg cgcagttgct gggttccgac ccgaactgcc ttgccgagaa
     4981 cgctgccagg ctggccgccc tgggaccgca cggcatcgat ctcaacttcg gttgtccggc
     5041 gaagatcgtc aaccgccacg gcggtggggc ggcgctgctc gacgatcccg aactgatcgc
     5101 ggccatcgtc agcgcggtcc ggcgcgcagt gcctcgcacg atgccggtgt cagccaagat
     5161 gcgactcggc aatctggacg gcagccgcgc ggtcgagtgc gcgcaggcga tcgccggggc
     5221 gggcgccgat gagctcgtcg tgcacgcccg cacgaaggcc gatggctacc ggccgccggc
     5281 ctactgggag cgcgtggccg aagtccgtgc ggcggtgagc atcccggtcg cggccaatgg
     5341 cgagatatgg accgtggccg atgcattgcg ttgccgggag ttgtccggct gcgatacgct
     5401 gatgctggga cgcgggatgg tggtcgatcc ggggctggcc ctcgcgatcg tggccgcaac
     5461 gcggcaggcg ccgctgtccg acgcagctgc gcgtggcatg ccgtgggagt ccctgctgcc
     5521 gctggtgacc gaattctggt cgctggtgct cgcacgtcgc gacatcgccg atcgacatcg
     5581 cgcaggccgg ctgaagcagt ggctgaatct cctgcgccgc tgccatccgc gcgccgaggc
     5641 gatctacggt gcgatccgca ccgtcaacga tccggcgcag atcggcgcct acctggccga
     5701 gtccgtgcgg atcgcagcct gagcggctgc ttttcgttgc cgaatcgcgt cgtgctgcca
     5761 acagcgacac gcgttggacc tcgccgaggc gaccgcccgg cgccacaatc gtccggtcac
     5821 cactgactga gttgcccgcc tcgatgccca cgtccgttcc gccggtcaag acgccgctcg
     5881 gtcacgagga gttgcgaacg cgctcgcacg ggctgaacca gcgtcatcga acgatgctgt
     5941 ttctcgtcga tggacgccgc cccctcagcg aagttctcgc gctggccctg caggcgggtg
     6001 ccagcaccac gcatttcgag gatctggtgc acctcggact ggtcgagctg gtgatcgatc
     6061 ccgacgaggc ttccgaggtc atgccgagcc agcaaggtgc cctcgacagc ggcggcgcca
     6121 gcacccgctc gggtgtgctg gcggttgagc tcggaccacc ctcgtcgcct gttgagccgc
     6181 cgcctagagc acccttgcct cgccctgtgg tcgaggttct cgaggtcgat gtcgatgccg
     6241 tgccgccacc ctcccggcct gtgcccctga atgcagacca cacgggatcg gcgctgtcgg
     6301 ttccgcaacg ccgggcgctt gccaaggcct ccgatgacga gttgttccag caggtgcgcg
     6361 agttgctgat cgacaccttg cgtgtcgatg cgcccgtgtt ctccgcgatc accctgatgc
     6421 gggttcaccg tgcgcgctcg tccaaggaac tgatcaacct cgtctgggag atcgagcgcc
     6481 atctgggcgg gccttcccgc aggcgccgcg agatgtcgag cctgcatcgc gctcgcgaac
     6541 tgctgggcat gggcaacacc caggtcagcc tggacgccga agagaccggg ttccccgata
     6601 ccgagtgatc tgttctcgcg cggcgtcgtc cgacgtgcga aagcgccggg ggccgaccgg
     6661 atgccggtgg atgtcggtcg acattgggtg gtgccgcgca cttcccttct cgccgatcgt
     6721 caacgtcgag aatcgacgcc tcagcctgct cgaccgaact cgaacaccga ggccattccg
     6781 acgccgtgac ccgcaccgat gcattgaacg actcttcccg ccggcgccga tggctggcgt
     6841 tgctggtggt ctcgctgacg gcgttcacgg ccatcgcatt gaacgcaggc tccggatcgc
     6901 acctgcaggc gatcgaccag caggcttcgc agcacctgct cgcgcacggg tccagcacct
     6961 ggactctcgc aatgcagtgg cttgcaaggg ttcacggtgt cgcaggcatc gcgatcgtcg
     7021 gcaccctgtg gctggcgtgg ctcgggctcc gcggcgaaac gatgcagctt gtgaggctgg
     7081 cgctgatcgt cggaggcggg ctggtgctca acagtgtgct gaagcgcgtg ttccaacgcg
     7141 cccggccggt gttcggcgag ggagtcgaac tgccgctgga gacgctggcg acttacagct
     7201 tcccgagtgg ccacgtgtcg ggcagcgtcg tcttctatgg cttcgtgctg atgacgatct
     7261 tctccgcgac gcagcatcga gcctggcgct cggcgtcggt ggtcgtggcg ggcaccatgg
     7321 tcgtgctgat cgcctacaac cgcgtctacg tcggggccca cttcgtcagc gacgtgctgg
     7381 cggcggcggc cgaggggctg gcctggctcg cgttctgtgc gctgctgctg gagcgcgtgc
     7441 aaacccgtcc gtcgaagtga acctgcaccc cgcggatgcg tgacctgatt ccagtgatcg
     7501 tcaacagcgc cgcgcgcagc ggcgactcgc gtgcgcagat cgcggcattg cccggactgt
     7561 tcgagagcgc cggcttgcgc attcgcctga tcgacctctg ccgtggcgcg gactccgacg
     7621 ccgagatcga cgcggcgctc gccgcacggc cctccatggt ggtggccggt ggcggcgacg
     7681 gaacgttgag cgccatcgct gcccggctgg tcgacaccgg cgtcccgctc ggggtgctgc
     7741 cgctgggcac gctgaaccat ttcgccaagg acctgggtat tcctctcgct gtcgatgctg
     7801 ccgtgcaggt gatcgcggca ggtcacacgg ccgatgtcga tgtcggtgag gtcaacgggc
     7861 gcatcttcat caacaactcg gggctgggcc tgtatgcgga catcgtccgt caccgcgatc
     7921 gtcagcagcg cacgctgggc cgtggcaagt ggtacgcact ggcctgggcg acgtggtcga
     7981 tgctgcggcg ctatccgttc gtgcacgtga ggctggtcat cgacggatgc tcacgcgaat
     8041 ggcgcacgcc gttcgtgttc gtcggcaaca accagtacgc gatggaaggg ctgcgcatcg
     8101 gcgtgcgcag ccgcctcgac gaaggcctgc tgtgcgtcta tgtcgcgcag cagccgggac
     8161 ggctcggcct gttgcggctg gcgttgcacg cgctgctcgg tcggctcagc caggcgagcg
     8221 atttcgatgc gatgacagcc accgagctgc tgatcgaaac ccgacgaagg cacctgcacg
     8281 tggccgccga tggcgaggtg tggccgatga ccacgccgtt gcgctaccgg acgcggcccg
     8341 gcgcgttgcg tgtcatcgtg cctgcgccgc catcggctga gacgtccggg ctcgattccg
     8401 gctgaagcgt cgggattcga tgcgctggaa ggcgctgtgt tctgcgctgt agtcccagcg
     8461 ctcgatggtg tacccgacgt ccccggggtg cccggtgcgg atcacgttca ccgagttcgg
     8521 cacgtcacgc cgcacgcggc tcgacagcgc ggtgcccgcc tgcgcgacga cgaggcttgc
     8581 cccgcctgcg cccgcaggcg tcagctcgcg catgtagggc aggtggatgt gaccgcccag
     8641 cgcgacatct gcgccggcgc cggcccattg ccgcagcgcg gtctcgcatc cgcgaagcag
     8701 gtgcttgcgc tctgcgtcgc cctcgacgtg cagcggctgg tgcacgacca ccacgcgcag
     8761 ctgtccgggc cgcgcgctgc gcaatcgggc agcaacccgc tcgatctgcg caggcgagac
     8821 ctgaccttcg acgtggcgca ggcggcgcgt ggtcttcacg caaagcacca gcaggtcggc
     8881 gctgtcgtgc gtcggctcca gtgcctcgcc gaaggcctcc tggaaccgtg catacggaaa
     8941 cagcaggcgc gccacgccgt tgaacagcgg gatgtcgtgg ttaccgggaa tggccagcca
     9001 gggaacgcca aggcggtcga cgaaccgccg cgcgctggcg aactgttccg gcgtggcacg
     9061 ctgggtgatg tcgccggaca gcaccagcag ctcgggtgcg aggtcgctgc tgagccgggc
     9121 cagcgcctcg acgaccggag cctgctcggt gccgaaatgc gtgtccgaga tgtggaggag
     9181 ggtggtcaag ggcaagcgga cgagatggtc tccgggtcgc cgggcagagc ccggcgatca
     9241 gttcgtctgc tggatgccgg caatcgccca ctggctgccg tcgtttggct tcgcgaggtg
     9301 ccagacctcg tcgaaaggct gggtcggacc ggacgcttct tccttgatca ggccatggaa
     9361 gcgcacgctg acgacctgcc gatcggcctc gtcggagaag tcgagcactt cggcgtcgac
     9421 ccggacgaca tcggtctgct gcgcgctgct gccgcgctcc tgcaggtcga gcttgaccgc
     9481 agcgtacatc tcgggcgtcg tgaactggcg caggtcgttc aggtcggcgc tgtcgttcgc
     9541 ggcctgcatg cggatgaaga tcattttcgc gatccgctcg aagcccgcct ggtcgaagcc
     9601 gacaggcacc gtcggtggcc gcaggtaagg ctggttcagt gccggtgaag ccgccaccgg
     9661 ttccggcgcg ggttgcgggt tccagccgga acctgaaccg ccagagccgg cagctgcacc
     9721 cgctcctgcg aactgcatgc cgcctggagc ccgcatcgga ctcgcggacg atttggcgcc
     9781 catgcggctt atgacgacgc ggatcgcgac gaaggccacg ccggccagca gcagcatcat
     9841 caggatgttg ccgaattcgg cgcccatgcc caggtggctc atcagggccg cgatgcccag
     9901 tccggccgcg agtccggcga tcggacccat ccatgagcgt ttgggggccg ccgcgggcgc
     9961 ggctccggcc gtggcgggcg cggtcggtgt tgcctgggcc ggcttggccg gcgtggcatc
    10021 cggagcgctc cggcttggca ggctgcgctg catgcccgag gaactgccgc cgcccaagcg
    10081 tttggcgtcg acgctcaatg gcgccaggcc tgcggccatg actgcgactg acagagccag
    10141 gatccaggat ttcatcttgc gttcccttgg ttttggttca cgaggagagg atgcatcgta
    10201 gcggcagagc gcgtcaggcg cgccggcgag gccattcaca ctcctcgaca tggggccgcc
    10261 ctgatgaatg tcaatgtcca ggcgttctca ggcatcctgc cgcggcggat atgtgtggcg
    10321 agtgatgcat catgggcgtt cagacgacaa cggaggccac cgtcagatga cacacgccct
    10381 gatcgtcgat gacgatgtgg attcggcttc caccctgaag gccctgatcg tgggcgaagg
    10441 cctcaccgtg gcggtggtgc acagcctgcg cgacgcgcgg cgccagatcg ccctgcagca
    10501 gccggacatc gtcctgctcg atctgcaact gcccgacggc aacgggatgg atctgctcgc
    10561 cgaccggcac atgctggccg attccgagat cgtgctgatc accgggcatg ccagcctcga
    10621 gacctcgatc caggcgctgc gactcggtgc ggctgactac atcgtcaagc cgatcaacat
    10681 gcgccagctg cagggcgtgc tgtcgcggtt catgaagccg gcggtgctga aggccgaggt
    10741 cgaagggctg gtggcgcagg tcgaacccac gggccatttc ggtcatctct gggggcgggc
    10801 gccgtcgatg ttgcgccttt acgagcagat cacgcgtgtg gccggcacca gcgtcacggt
    10861 cttcatcacc ggcgaaagcg gcagcggcaa ggaagtcgtc gcgcagacgg tgcacgacct
    10921 gagtcgccgc cgcaagcagg ccttcctcgc gatcaactgc ggcgcgatct cgcccaacct
    10981 gatcgagagc gagatcttcg gccacgagaa aggcagcttc accggcgccg accggcagca
    11041 cctcggcttc ttcgagcgcg ccagcggcgg tacgctgttc ctcgacgaga tcaccgagat
    11101 gccgatggaa ttgcaggtca agctgctgcg ggtgctggag accggccgct tcatgcgggt
    11161 gggatcgacg cagacacagg aaaccgacgt gcgcgtgctg gccgccacca accggtcacc
    11221 cgagcaggcc gtcgcatcgg gaaaattgcg agaagacctg ttttaccgat tgaatgtttt
    11281 tccgatcgag ttgccgccgc tgcgtgatcg gctcagcgat gtgccgctgc tcgctcggca
    11341 tttcctgaca gccttcgggg cgcagcaggg ccaggtcaag cagttcagcg cggccgcact
    11401 cgaccggctg agccgctacc gctggccggg caatgtgcgc gaactgcgca atgcggtgca
    11461 gcgcgcgtac gtgatgtcgg ccggcgggct gatcgacgag gagtgcctgc ccgacatggc
    11521 ctttgccgtg gccccggctg cagtcgctgc ggctcgggtc gcggagtctc cgccgcgcga
    11581 tgccgtgcct gcgcagatga cggatgacag catcacgctg ccgatcggta cctcgctggc
    11641 tcagtgcgaa cgggcattga tcctggccac cttgcgggcc taccaccacc acaaggaacg
    11701 cacggctgcg gcactcggca tcagcctgaa gacgctctac aaccggctca aggagtacgc
    11761 cggggacagc acgggaaagg ccgtcgatcc ggattgatgc gcaatgcgcc ggtcatgacg
    11821 tgactgcggg cggcatcacg gcagcacgca gtccgctgcg gtccgcaagg atcaggcgcg
    11881 acacgctggg ccagtcgacg cagcgtgcgt ggggtatgcg caagggtgat cgccgccaga
    11941 ccgtctcgcc gccatcgtcg agcagcacgg tgcggcagcc ggcgcgccga ccggcctcga
    12001 cgtcgtcgag cttgtcaccc accatccacg aagccgacag gtcgatgcgg tgcgcccgag
    12061 ctgcccgcac cagcattccc ggtgccggct tgcgacacag acaggccggt tgtccaccgg
    12121 cggccggcac gtgcgggcag atcacgaaat ctgtcagttc cacgccggct tcgtctcgca
    12181 ggcgtcgctc cagcgctgac tgcagtgcag agaactgtcc gcgcgtgaag tagccgcggc
    12241 cgatgccgct ctggttgctg accagcacca gtggcaggcc cgcggatgcc agcatcgcca
    12301 tggcttcgca ggccgacggc ttgaatcgca gcagcgccgg gtcggcgttg tacggcacct
    12361 tctcgaccag cgtaccgccc tgatcgatga agaccgccgg tcgcggggtc gacgctgcgc
    12421 tgtacggtgg agcgaccgtg gtcagtgcaa ccctcagggc atgttcgttc atggggcggg
    12481 caggggagca agttcgttct cggtgatggc aatcggtgtg cccacgagct gagcgagctt
    12541 ctccggacca gtgtcccggc ggttgatacc gcctgaacct gcgagctggc ttgttgcgcg
    12601 gcatgccgct tgccccttgc tgcatgaagc atcaggcgca gcaagcgtcc cgcgccttcg
    12661 tcgccgaacc cttgcagcgc aggcgttcgc gacgagcggc tgcaccccac accacagcca
    12721 cgaacatgaa acacatcgac tcgacctgca gcacagccta ctcgcatgac tgccatgaac
    12781 ccgccggaga cgacggcttc tgtttcgacc tttgcctggc gccgttgctt cctgccggtg
    12841 tcgaaccgtg gctctcgacg cgcgcgatct tcttcgtcga tggcgagttg cccgtctcgg
    12901 gtcgacttgc gttcgacacg ctgaatgcaa ggcgttcgcc ggagttcgtc agcgactgaa
    12961 cgtcgccgcg ccgcgcacac gggcgcacca cttctgcagc ggggctccga gccgtgtcct
    13021 ctgcaggggc aaccgcgccc ggggccgaag taacagtggg taattcatcg agatttcgcg
    13081 cgccgaagca atagcattcg gcccgtcggc atacccgtgc gaaagccggg gacgcaaagt
    13141 ttccggtcta acgccccgct tgagcggtgg ccacggcagc gggaccgcca gcagtcgctt
    13201 gatgcgactg ccgagggttc tgcctccgtg tccaggaccg ggttgttcag gcaggtattc
    13261 ctcgaaccct cagcacacca tgaataagat tctcagaacg ctctgcggac tcaccttgtt
    13321 tctggggctg tgcgcctctg cctggtccga cggacatcgc gcagaccggg tgccacacgt
    13381 cgagaagccc cgcttcccgc agctcgacct tcccgatcgg cattccggcg ggcagcgggc
    13441 catcgatggg ctcggcacgc ggctttccga agtggcgaac tggtacggca agtcacccga
    13501 cgagttcagg ggcatgctga tgaaggaccg ccgcatgcgc ctggacaagc gcgggcgcgt
    13561 gttcatcgtc gaggaactcg accagccgat gcacgcaacg cccgtttcgg cggcagacac
    13621 cagcctgctc gatggcaccc tgctgccgct cgaccagacc ttcctgttgc acagccgtcc
    13681 gggcgccaag cgcacgatct acctgaactt caagggcgcc accctgaccg gcaccgcctg
    13741 gaacggcacc ggaggcagcc tgacggcatt gcccttcgac atcgatggca ttccgtacac
    13801 cttctccacc accgagttgc agcgcatcca gtacatctgg cagcgggtgg ccgaggactt
    13861 cgcccccttc gatgtcgacg tcaccaccga ggcgatcgcg ccggaactga tcacccgtgc
    13921 cggatcggcc gacacggtgt tcggcaccac ggtgctgatc accaagcgca ccggtgtcta
    13981 caactgcagt tgtggcggcg tggcttacct gggcgtgttc gacgacaccc gcgagttcta
    14041 caagccggca ctggtgttct acgacgcgct tggcaacgga aacgagaagt acgtggccga
    14101 ggccgtctcg cacgaggccg gtcacaacat gggtctgggt cacgacggca ccgccacggc
    14161 aggctattac caggggcagg gcagcggcgc caccggctgg gctccgatca tgggtatcgg
    14221 gtactaccag cccgtggtgc agtggagcag gggcgaatac accggcgcca acaacgtgca
    14281 ggacgactac gcggtgatgc agagcaacgg actgccgctg cgcaacgacg atcacggcaa
    14341 caccctcgcc agcgcctcgg tgctgatcgg tgccgccagc ggtggcatga ccaccacttc
    14401 ggcgcagggc gtcatcgaac gacccacgga cgtggacctc tttgcgttct ctgcgggtgc
    14461 gggtacggcc acgttcaagg tgacgccggc ggcgcggtcg accaacctgg atgcctgggt
    14521 gggcttgcgc gacgcggccg gcaacctgct ggccaacatc aaccccgtcg atgcgctgaa
    14581 cgccatcgtc accttcaatc tgccggtggc cggtacctac tacctgtcgg ttcagggcac
    14641 cggcaagggc gacccgctga ccacgggcta cagcgcctat ggcagcctcg gtcaatacgc
    14701 ggtcagcggc aatttcttca cgcccggcag tcagccgccc acctcggtga tcagcgcgtc
    14761 cacgctgcgc ggagtggcgc cactggccat tgcgttctcc ggcgcggctt cccgcgatcc
    14821 ggacggcagc ctggtggcct acgactggac cttcggtgac gggggcagtg ccagcggtgc
    14881 gacagcctcg cacacctaca gtgcgccggg cagctatgcc gccgtgctgc gtgtcaccga
    14941 caacaccggt ctgaccgcgt ccagcacggt cacgatcacc gtggatgctc cggtggtggt
    15001 gcagacgatg cgcgtcagcg acatcgcgat gtccttgagg gtgtcccgca ctggcagcgc
    15061 tcaggctggc gcagccgtca aggtggtggg cagcaacgga ctgccgctgg tcggtgccac
    15121 ggtggccggg gcctggagtg ggacgtttgc gcgcagcgga tccgtcgtca cagacagcac
    15181 cggcgtggcc cggttcagtt cgccctccac caagagtacc ggtgccagct tcatcttcac
    15241 cgtgaagggc gtgagcggtg tgaccgggta cacctacgcg ccgctgacca acaccgaaac
    15301 cagcgactcg atcgctcgct gatccgactg tgctgcagtg cccgctccgg cgggcgctgc
    15361 cacgtcgccg ccctcgggct gcccccgata tcgcagggat ctgcgttcag caggcgggca
    15421 gcagtatcga cacggtggtg ccgagccccg gggtgctcgc gcggcgtgat gtgccgcctg
    15481 atcggacgca gaacgcgtgc acctgctgca ggctcggggt cgggccggtg gattcaccgt
    15541 ctgccgtccc gtcgtcaccc acggtgatca agacatagtc tccggctgcc aggtcgtagg
    15601 tgtcctcggt ggcggcgttg caggcgtgca ggcgcagccg gccccccgaa ggcatcgcat
    15661 cgcgcgcgtc cagcgtcagc gtggtgagtg cgagttccag tccgttggcg tcgaccatcg
    15721 cgtgccgcgt ggttgcggcc accgcaaccg acagcgtgat gcgtcggccg gcgatggctc
    15781 tcaggaattc atcggattcg gacagcatgc tcgacaggtc gaccgcgtgc ggcggcgacg
    15841 gctgtcgatc ggaaaagcgc atcaggtgct gcgtcagccg actgcctgcc tccaccgcgc
    15901 gaagcgtggc tgccaccggc gcctgcagct ccgcggcctg aacctgccgc tcgatcagat
    15961 gagcgctgtt gctgatgacg ccgagcaggt tgttgaagtc gtgcaccacg ccaccggtca
    16021 gtcgacccag cgcttcgatg cgattccggt ctgtggtgct cgcttcgtcc acccggtcgg
    16081 gcagcggctc cctggcgggc ttctgcaaag gcgattcgga cggctcgacg acgggtcgcg
    16141 tcgcgaccca ccacaggccg gccagggcca cgccgagcag gcacagcgcc cagatggcca
    16201 cctgcagggt ggcgctggca gcccagtcgg ccggcggcgc gtgccgttcc caccgccagg
    16261 tgttcgcagt gacccaagcc accgccagca ggcctggcag cagcactgac agcatcagga
    16321 gcgtcatgcg ggatcgaggg gaagcgttca tggatgcgag cgtgacacct ttcggtgctt
    16381 ggcggggtgg tttgacgagc gatcgatgcg cgcgatgcgc atcgatccct gcaccttcag
    16441 acgctgtctt cagcgcgctg cgatgcgccc ttgcgcatcc gagaacagca cctcgacccg
    16501 acggttctgc tggcgaccgg ccggcgtgcc gttgtcggcc accgggtttg cttcacccca
    16561 ggcacgcacc tcgatgcgct cgacagcaat gccggcattc gtcatctggc gctggaaggt
    16621 ttcggcccgg cgctgtgaca gctgcaggtt catctcctcg ccgccgacgc tgtcggtgaa
    16681 tccctcgatc agaacgcgcc gctccgggtg gcggcccagc acctgcacca gttgttcgac
    16741 gctgcgtcgc gcgccgggct gcagcgtggc gcgcccagtg tcgaacagca cgtcgcccag
    16801 cgtgaccacc atgccgcggt ccgtgctgcg gcctttcagc tcctgcaggt cctgctccag
    16861 tccggccgcc cgttcggatt gctgaaccgc cagcaggcga gccgcgtcgg cctgctcgcg
    16921 cgagagcacg gcttcgttgc gcgacgtctg cgccgcgccc tgggctgcta ctgcgctcgc
    16981 cttggcggtt cttgcatcgg cctgcgccac gcgcgcgttc gcggtggcca cgtcggcctc
    17041 gcgcgagcgg gcatccagct gcacccgctc gcgttcggca ccggccttct ggatccgctg
    17101 gtccgcttcg gcctgggaac cgacggcaag cgcgatctcg gctcgctggc gcgcgatgta
    17161 ggccaggtgg cgtgtctcgt cgacattgcg atcgttggcc cacgctgttt cggcggcgtt
    17221 catcgcctgc agcgcgcgtt cgagctcgac cgcgccgctg cgggccactg ccgggttgct
    17281 gcgagcctgc gaaagcgtgg tgcgcgcggc gaccaggtcg gggttctgcg gtgcggtggt
    17341 ctggcaggcg gcgagtgcca gggcgatcga cgctgtcaag agggtgcgga tggttttcat
    17401 ggggtgtcct ggtcgttcgg aagggttcac tggggcgcgg tggcgggggc cgacacgggc
    17461 tgcttcgccg ccgcgcggtt gagctcgtcg cgcagcgtct gcaggcccgc ctcgagttcg
    17521 gtcacggcgc gacgcgaacg ttcggagccc gcccgggcac gcgccagctg cgcatccaca
    17581 tcggcttctt cggcgagccg gatggcgccg cgctcatcgc cttcggcagc gagggctcgg
    17641 gctctatcca gcttgctgcg cgccgcgttg agttcggcgg ctgccatctc gggcgcgccg
    17701 gccgagcgcg cggcctcgac agaagcagcg gtggcggcca tggacggggc cggtgagggt
    17761 gtgtgggcgc agccggtgac ggccagggcc cccgaggcca tcagcgaggc gaggatcaag
    17821 gttttcatgc ggggtctccg tggagttgag acctggcgga tgccaggtga tgacaactcc
    17881 gcggcaatcg acgctcccag cagcgtcggg catcggcgct cgcaggtgcg aggcggcagg
    17941 cgggtgcgct gccgccgggt gacgtcagcg gatcgcctga gcagccggtg gctgcaggtc
    18001 acggggggaa ttggatgtcg gccccgacgg tctcccgacc tcggggcggt gccgactccc
    18061 gacgctggtg cgtcggcggc ggtcgtcgct cacggcgtgg ccgtgaggtg gggcgggctc
    18121 tggctcagcg tccggccagc cgcccggcct ggcgcgttgg cctcagccgc ggcgctcttc
    18181 gctgcggtag aactcctgga tgctgttgcc gatcgcgtcg ccgcccaggt agccggcgat
    18241 gccgccgagc gtgccgagga tcagcatggc ggcctcgctg ccctgcgagc ggccgacata
    18301 gccggcggcc acgcccacga tgatcgtgcc cagcaggctg gtgatcgggt gtcctccggg
    18361 gattccaacg aaggtgccgt tggtgccgta ggtcttggtt ctgctgctgg ccatggggag
    18421 atctccgaag ttgggtgcga catgtccggc atcctgcttc aggatgtcga gaaccggaat
    18481 ttacgccaga gcggcctcgc gaggaacctc agtccccgtc cggcaacgcc ttcgtttcca
    18541 ccaatggcaa ggtcaccctg aattcgcttc cgcagccggg accggcgctg aaggcctcga
    18601 cctctccgcc gtgggctttg acgagttcgc gcaccacggt cagtccgagg cccaggccgc
    18661 tgccgtcgaa gacggtggct gcgctgtcct gcacgaaggg ctcgaagatg tggctcagcg
    18721 cgtttgcatc gatgccgatg ccgttgtccg acacggtgat caccagcgac tcgtcgatgc
    18781 gcaccactga cagcctgata tagccgccct gaggcgtgta cttcgatgcg ttgtcgagca
    18841 ggttgcgcag cacctgcgac aggcgcaggg gatcaccgta caccggcaac tcgcccagat
    18901 gcgcctggac gctgaactgt tgcagccggg tgtcgatggc cgggcggcag gccgcgacca
    18961 cctcgtcgat cagcccggtc atttccaccg cgcggcgctc cagccgcagc ttgccgctgc
    19021 tgacccgcga cacatcgagc aggtcgctga ccagccgcga catctgcacc agttgccgtt
    19081 cgatgatgga ggccatcttc ggcagcacca gggcgtcgcc gccggtgcgc ccgatcaccg
    19141 cggaggccga gcgcatcgac atcagcgggt tgctgagctc gtgcaccgcc cgcgacatga
    19201 actgggtctg gcgcctgagc gccagctccg cggcagcctg cagctcccgt gcgtccagcg
    19261 cgctcaacag cagttgctgg ttcgcctcct gcagttgcgc ctgccgctgc tcgaattcct
    19321 tgcgggcgct ctcgcggtgc gacagtggcg atctcagcga ggcgagcggc ggcgtctgca
    19381 ccaggggctg tcccaggctc tggccgtcgt ggaacacgaa gctgcccatg ccgtgtcgct
    19441 tggcgcggta catcgcgccg tcggccagcg cgatcaatgc actcgcgttg tctccatcgg
    19501 aggggtagag gctgatgccg atgctcgcct tcatgcgcat cacatgcgag ccgagcagcg
    19561 ccggtgcgcc cagcgcagcg atcaccttgt cggccaccgc ggcggcatcc tgtgcctgcg
    19621 acaccccatc gagcaggatc aggaactcgt cgccgccatg ccggctgacg gtgtctgccg
    19681 cacgcaccga agccaccagg cagcgtgcgg ccagttgcag cacctcgtcg ccgatcgcgt
    19741 ggccaagcgt gtcgttgacc tgcttgaagt cgtcgaggtc gatgaacagc aacgcgacct
    19801 gcttgccact gcggcgcgcg ctggcgatgg cctgctcgaa gcggtcgagc agcagcacgc
    19861 gattcggcag ctgggtcagc ggatcgagct cggccagtcg ggctgcacgc agcatcccct
    19921 gcgctgcggc ttcggcatcg gcacgcgact gcagcaccgc aacgatgaga ttctcgttgg
    19981 cctcgcgcag ccggtcgccc tcgccctcgc cgagccggtt catcacctct gcgagcttgt
    20041 gctgcatcga ggtcagctct gcccgtgcat cctcgatgcg cgactgcagg cgggccagct
    20101 cgcgggctgg cccgtccgtc gcctcaccgc ccgggtcgtt attcatcagc gtgaccagtc
    20161 ggtcatgacc gtcggcggct tcagtccgcc cgaccggtcg gcatcgccgc accgtcgatg
    20221 gcgtggcgtt tgcgcgtcgg ccttcctcca agcagccctt cctgatcgcc cagcgtctgg
    20281 ccgatgcgga tgccttcgtc gccgatggtg aactcgcgca actcgtccga gtgcgcgctg
    20341 gcccgcacct tgaccaccgc catcacgcgg cgcagccggc tgtcgacctc gatgtagcgc
    20401 tgcacgatga tggcgtcggt caggaaggcc gtgccgtaag ggctgaagcg caggtcggtg
    20461 tagcggtctt ccagctccga ggtcatcagc acggtgacgc ctgcgttggc cagcgcggtg
    20521 accaggcgcg acagcgattc gcggaagtcg tcgcggaagg tcggtgccac cgccagctcg
    20581 aagccggaca gcgaatcgat gacgacgcgg gttgcgccga gccgccggat ctcgcgggtc
    20641 agcagcatca cgatctcgtc gatcgacagg tcgggtgcgt ggctgtcgac cagggcgacg
    20701 cgtccgctgg cgatcagatc ggccaccagc gggttgcgcg tccggttggg tcgttgctcg
    20761 aatgcggcga tcacgccggt ttcgcccagt cggttgcctt cggccaggaa ggccgccgcc
    20821 aggatgctct tgcccgatcc cgagggcccg gccaccagca gcgagtagcc gcgcggcagt
    20881 cccccgccca gcatgtcgtc caggccgggc acgccggtca gcaggcgcgc ctcgggcgat
    20941 gtgttgccgc tcggggtcag tccgttcagg tgtggcagcg cttgcgccat ctggtcggcg
    21001 ggtgcgaaca cctcgatgcc ggcattgccg atgcggaagg tgtgcagccc cggcagcgtc
    21061 ggctggccac gcatcttcat gacctccatc ttgcggacca tcgagttgcg ctgcacgctc
    21121 tggcgcagcc agatcaggcc gtcggcgacg gtgaacaccg gattggcgtc ggtctcgacg
    21181 aagtactcgc cgatcaggaa ggtggtggcc tgccagctcg tcatcagcat gccgagttgc
    21241 tgcacgaatt gctgcaggct cttctccggg ttgttcggcg gggcggccga cagcaccacg
    21301 gagcggaacg aatcgacgaa caccagcgcg ggaccgtagg cctcgacctc ggccacgatg
    21361 cgcgcgagca cccggtcgag atcgcccgtc agcgtgtcgt ccgacaggtt gatgaagcgg
    21421 atcgaccggt tgacggcctc gctgtcgaag aactcgaact gctgctggta ccgcagcatc
    21481 ttcagcggcg gctcgcccag cacggtgaag tagatcgccc ggaactccgg cttcgccagc
    21541 gcgaacatca tctggtgggc cagcgtggtc ttgccgcagc ccggcggccc ggcgatgagg
    21601 ttgaacgaca gttcgggcag tccgccgccc agcacctcgt cgagcccggg tacccccgtg
    21661 gccagacggt tgatggtgac tttgttcatg aggtcgtgtc ctgcgcgtgt gcggccgggc
    21721 cgtgaggaga aagcgcccag accgggcgca gcaggcgctc ggtgagtgac gggccgacca
    21781 tgctttcgag caggtcgtgg agggcgtgga acatggcgca cccgccggcc acagcggccg
    21841 cgccggtctg ccgtgcgaac gcggtcctga tcctggcaag gtccatcgcc ggtggcacgc
    21901 cttcctgcaa gccatcaagc cacggatgga cggtggcagc cacgtacagg ctgcgcttgt
    21961 agagcgcgac gagaccgcgc tgacccacga tgggcgtcag tgccgcatcg atctcgagcc
    22021 agatggcctc gacggcttcg gcgacctgcg aggcctcggc gtctgccctg accctgttgg
    22081 ccagaggtgc agtgatgagc ggtctttccg gtgtttccat ggacatgtgt caaagaccat
    22141 ttacatggat tgattgatcg tacgccacgg gctcagcggg acacggtttg tcgcgatttc
    22201 tactgacttg ccgcaagaga ttccgattcg gcaatccgct ccttcccgga attcagcgtg
    22261 accacgacgt tcgcagtgtg accgtgcggt gcgggggggc agtgatctct cgtatccacg
    22321 ggtcaatcgc ctttcatggg tgggccgcgg ttcgcgcggc acgcaggttg cggtgaagca
    22381 ggtcagaaca tgtgcaacga ggacctgctt cgatgaccaa cctgggcttt cgagccgatc
    22441 accagcagac gcagcggcag tcgctgtcca cccgctttca gcaagctgtg cgcctgctcc
    22501 agatgtcgtc gctcgatttc gcggccagcg tgcgcgatgt gctgggcaag aaccccttcc
    22561 tcgaagtcga ggagggtgac ggcgaggccg accaggatgc ggctgcagac gacccgggcg
    22621 acggagccga ctccacggcg gacagcgatg ggctgtctgc ggagcttgcg atgccgcgcg
    22681 agggcctgga tgaagtcggc gaaagcgaca acgaccgcga tctgtggcgg gccgattccg
    22741 gcgtgtcccc ccgctcggcc gatgacggcg agctgtctgc gatgaaccag atggccgtgg
    22801 agacgtcgct caatgcccac ctgcacggcc agctgaacct gctgccgctg agcctgcgcg
    22861 agttgctgct ggcgcgcgtg gtcgtggagt cgctcgacga cgatggctac ctgcgcacgt
    22921 cgctggacac cctggtgggg ctcgaagcga tgtcgccggc cgccacgttg accgagatgc
    22981 gtgcagcgct gcgctgcgtg cagtcgctcg acccgtgcgg cgtggcggcg tgcagcgtgg
    23041 cggaatgcct gctgctgcaa ctgccgtcga tcgactgtgc acagacgcgc gcactggccg
    23101 agcgcatcgt cggcgaccac gtcggtgcgc tcgcggcacg cgacacgcac gggctggcgc
    23161 gcgcgcttgg cgagtcaccg gtgcgcatcc aggcggtgtg cgaccgcatc cggcacctcg
    23221 acccgaggcc gggctggcgc ttcagcgcgg agcgcgtggt gtacgtcgtg cccgatgtga
    23281 tcgtccgcaa ggtgcgtggc gtctggagcg cgcagctcaa cccggcgatc gttccgcgcg
    23341 ttcgcctgaa tcaggtctac gccgagctgt tcaagcgtca ccgcaccgcc gacaacgccg
    23401 aactggccga ccacctgcag gaggcacgct ggaccgtgcg caatgtcgag cagcgcttct
    23461 cgacgatcct cgacgtggcc gaggcgatcg tgaagcggca gcagcatttc ctcgacttcg
    23521 gcccgatggc gatgaagccg atgggcctgc gcgaaattgc cgacgaggtg ggcgtgcacg
    23581 agtccaccgt gtcgcgggtc accaacaaca agtacatgtc gacgccgtgc ggggtgttcg
    23641 agatgacgca cttcttttcg cgcgccatgg tctcgaccaa cggaagcgcc tgctccggca
    23701 cggcgatacg cggcctggtc aaggacctga tcgacgccga gtcgcctgcg gcgccgctgt
    23761 ccgatgtcga gatcacgcgg cagcttgccg agcaggggct ggtggtggcc cgacgcacgg
    23821 tgaccaagta ccggcagctg ctgcgcatcg aggcggtcga ccgccgccgg cgcctttcat
    23881 gagcgtcaac ccccccgcag gcacgtcgtt tgccgctgta acggcgtgtg cggcaacccg
    23941 gcgttgctgt ggtgtcggac cactgttcga cgggtctttc cgaggagcgc tgcgttatga
    24001 gtatcgatat ggaattggtc gacagctggc cggagtcggt ccgcgtcgag gttcaggttc
    24061 actacgccag cggcccgtgc gacgcgagga acttctcggt cgagccctgc ccgtcgcagc
    24121 atgcggtgcg tctgttcaat ctccggctcg attgcgagcc gcttgaaatg catcgcatcg
    24181 gctgggtcga tctgcctggg cgggcgttgt accagcgttg cctgagtgtg ctggcactgc
    24241 actggttcgg ttccatcgac atggtgcctc gcgttcgcgg cgtgcctttg tggcgcagcc
    24301 tgagtgccga actcacgatg ccttcggcgc acgtgaaccg ggtgaagttc gatatccagg
    24361 ccgtcaacga caccagcgcc acgctgcgtt accagttccg gcgagaccag ccgctggtca
    24421 acggcacggt gatgctcgac ccgctcgatg cggacccgct gcgcgccctg cttcgggtgg
    24481 gactcggccg ggtcaagcca atggctgttc cgcgcaaggc cgcatcgagg gcgcccgcca
    24541 ggtccctgcc gtctcattcc gccgccagcg ggcacgccgc ttcgcagcaa gcctagtcgg
    24601 gagttcctgc cgccatgagc gacaacgcgg aatcggctct gcccgacttg atccgaggcc
    24661 atcccctgct gagccagctt cgctccagct tgcaatcgga ggcagccgcc cacgaagggc
    24721 cggattcgtc aggctgggat ccggtcgcca gcgcgctggc gctgtccggc atgctgcccg
    24781 aaccgcctgc catcagcggc tgactggctg cttcgagtac caggcattca tgcagtcgat
    24841 cagcgtcttc gggtcgatcg gcttcgtgaa atgccggtcg aagcccgcgg ccagagcctt
    24901 caactgatcc tcctgctgac cccaacccgt cagtgccagc agcagcatgt cgcggccgcc
    24961 cggctgcagg cgcagacggc gcgccacctc gtagccgtcg aggtcgggca tgccgatgtc
    25021 gagcacggcg acctgcgggt gcacctgcac cgccagggcc agtgcacggg cgccgtcgaa
    25081 cgccacatgc acttcatggt gttcgagccc gagcagcagg cccaggctgt cggcggcatc
    25141 tcggttgtcg tcggcgatca ggatgcgcag cggcgtgacc actggtgcgg ccaggaccgc
    25201 gggcgcctcg tcgagagacg cgggtcggga gtcgaatgct gctggcggca gatggactcg
    25261 gaaggtcgcg ccgcctccga gccccgcgct gctggcgtcc aggtgcccgc catgcagttc
    25321 gaccagaccg cgcgacagcg ccaggccgat gcccaggccg ccctgcgtgc ggtcgctggc
    25381 gctgcgcagt tgcgagaaca tgtcgaagac gtagggcagt tgttcggggg tgatgccgat
    25441 gccgttgtcc tgaacctcga tcagcaactc gtgctcgtcg gcccgtgcgg ccagccggat
    25501 ctggccgccg ggtggcgtgt atctcgcggc gttgagcagc aggttggcca gcacctgggt
    25561 cacccgcacc tcgtcgacat gcgccacgca gcccggctgg agatcgacct ccagcgtgtg
    25621 tcgtctgccg tcgatcaaca cgcgcgcgtt ctcgacggca gcttcgacga tcgcctgcag
    25681 gcgatggggc tgcttctcca gttccagccg cccggccgag atgcgcgaga tgtccagcag
    25741 gtcgtcgagc agccaggcca tgtgccggat ctgacggttg atgacgtcac ggctgacggc
    25801 gagctcctca gccgtcaatg ccgggttggc aagcaccgac gcggcgctgc ggatcggcgc
    25861 cagcgggttg cgcagttcgt gcgacaggat cgcgaggaac tcgtccttgc gccggtcggc
    25921 ttcgcgcagt gattcggcgg tgcggcgcgc ctcggtgacg tcgatgtgca cgccgaccca
    25981 ctcgcgcaag tgaccgtcgg ccgcgaagac gggctcggcg caaacggcat agcgccgcca
    26041 ttgacccgcc gcgctgcgca gtcggtgctc ccattcgaag tggcgctgct gcttgaccgc
    26101 ctgcttccag gcgatcagcg tgggctgcag gtcatcggga tggatggcac tcgaccagcc
    26161 gtcgccccgg tattgcgccg gcgtctgacc agtgagggcg gcccagccaa actgctcgcc
    26221 gtccatttct ccctgcgcgt tctgcgtcca catcagcccg accgcgcgca cggcggcctg
    26281 gaagcgctct tcgctgatct ggcgcgcggc ctcggattgc accagccggg ctgttgcctg
    26341 atcgcgctcg gcctgctcgc gtcgctggac cgtggtttcg cggcacacgc agaacaaccc
    26401 tgacagggcg ccctgatcgt cgcggaccgg gtggtagccg aaggagaaat gggcttcctc
    26461 gacgcggcca ccacgcggga tcgaaagcgc gatgtcgtcc atctgcacgc tgtggccggc
    26521 gtagacgctg tcggccagcg ggccgatgtc ggcccagatg tcgctccaca catgctgcat
    26581 cggctgcccg agtgccgcgg ggtgccgcgc gccgaggatc ggcgcgtagg cgtcgttgta
    26641 gatgaagcag cgctgcggtc cccaggccac gaacatcgct tcgctggccc cgagcatcag
    26701 gcccgtcagc agtcgcagtt cggccggcca gccggctgcg gggccgagtc cgcagcggtc
    26761 ctcggcacgc agcaggcggg cgcactctcc ggcatggtcg aggaagacgg gggagggaac
    26821 ggcggacatg ttcggagggg cggagcgcgc cgcagtgtgt gcgttcccga tcgtcccgca
    26881 ctttgagggg gcgccccaac tatttcctcc ggggtcgaat cagcggcacg ccgattgcca
    26941 ctgaaggaca tgaaatgcaa ctgcaagacc ctctccggct tcccctcgtc tgctgcgcgc
    27001 ctgtggagcg tgccgttcga tgcgctgcaa agcgcgcgga agaatcggct gctcgcggcg
    27061 cttccgctgg ccgagtggca gcgatggttg ccgcatctcg agtgggtgga catgcctccg
    27121 ggtcgcgtct tgcacgagtc gggacacgcg ctcgatcacg tctatttccc gacgacggcg
    27181 atcgtgtcgc tgctgaacgt cacgcaggac ggtgcctcga ccgagatcgc cgtggtgggc
    27241 aacgagggcg tcgtcggcac cgcgcttttc atgggatcgg attccacctg cagccgcgca
    27301 gtggtgcaga gcggcggatt cgggttccgg ctgaaggcgc ggccgttgcg cgacgaattc
    27361 gaacgatctg gcgtggtgat gcacctgctg ctgcgctaca cgcaggcgct gatgacccag
    27421 atggcacagc tggcggtgtg caacaggcac cattcgatcg cccagcaact gtgtcgctgc
    27481 ctgctggtcc acctcgaccg gctcggcggt caggagatcg tgatgacgca cgagtcgatc
    27541 gccagcatgc tcggcgtgcg ccgggagggc gtcaccgaga ccgcactgaa gctggcggcc
    27601 gccgggctca tccgctatgc acggggccgg atcacggtgc tcgaccgagc gggtctcgag
    27661 cagcgcgcct gcgagtgcta tggcgtggtc agcaacgaat acgaccgact gctgagcgaa
    27721 cgcgtggcgg cctgagccgg ccgccgggcc aggcgatctc gaaacatcct gttgcaaggc
    27781 gggaaccaac aggggccaga ccgactcagc agttgcggcg agaggtgccg gtgcgcctct
    27841 atcattcgca ttgccgttgt ggatgccacc gcagccacgg caagaagcaa gccgaacatt
    27901 gcccatgtcc cgaccgatca ctgccgtttt cgcccgtttc ttcgctgctc tgttcgtcgc
    27961 cctggctttt tcgatggctg ccgcgcctgt ggcgcttgcc cagttccgcg tcgagatcac
    28021 cggggtcggc gcggcccagg taccgatcgc cattgccagg ttccgcgacg aagaccggca
    28081 gagccagccg ctggcggcca tcgtgcgtgc cgacctcgaa cgcagcgggt tgttccgcgg
    28141 tgtcgatgcg ccgggcgtgc tcgacgagtt gcgaacgccg gcgatggccg aatggcgcac
    28201 ccgcgcagcc gatgccctgg tcggtggatc ggtgacgaag ctggccgacg gccggtttga
    28261 cgtgcgcttc aagctgtggg acgtcgtcaa gggcagcgaa ctcggcggcc agagcacagt
    28321 ggtcgatcag gccgacctgc gactggcagc ccaccgcatc gccgacttca tccacgagaa
    28381 gctcaccggt cagaagggcg tgttctcgac gcgcatcgcc tacgtgacca aggtcggcaa
    28441 ccgtttcacg ctgcgcattg ccgatgccga cggcgaaggc gggcaggtcg cgctgaacag
    28501 caccgagccg atcatctcgc ccgcctggtc tcccgatggc cgcgaactgg cctacgtgtc
    28561 gttcgagagc cagaaggcgg ttgtctatgt ccaggaagtg gcgagcggca agcgccgcgc
    28621 cgtcgcgaac ttccgcggct cgaacagcgc gccggcgtgg tcgcccgatg ggcagacact
    28681 cgcggtgacg ctgtcgcgcg atggcggttc gcagctgtac cagatgggcc gcaacgggga
    28741 caacgttcgc cgactcacgc agagcatcgg gatcgacacc gagccggtct attcgccgga
    28801 cggacgcagc gtctatttcg tcagcgatcg cggaggcggc ccgcagatct accgtacctc
    28861 ggtcaatggg gcggcggtcg agcgcatcac cttcaccggc agctacaaca tcagcccggc
    28921 gatcagcccc gatgggcgca tgctggccta catctcgcgc cagggcaacg ccttcaagct
    28981 ccacgtgcag gacctgtccg gtggcgcacc acaggtgctg agcgacacca ccgacgacga
    29041 gagcccgagc tttgcgccga acggtcggct gatcatctac gcatcgcgct cgcagggccg
    29101 cgatgtgctg atgacgacca ccctcgacgg caagatccgc gcgcgcctgg tgtcctccaa
    29161 cgtggatgtg cgcgagccgg tatggggccc tttcgggcgc tgatgccgca gactgctcgc
    29221 aacgttgcct gcttcggata cccactgttt tctgttcact tcgacaaagg aaaaaaccat
    29281 gacccccttc cgccttcgtg cctgcgccca tcaacggttg ctctggacct ccctgtgcgc
    29341 ggtcgccctg gtgggctgcg gctccaacgt caagctcgac gagaacgcgc cccccgtgga
    29401 aagccgcagc ggcaccgctg tgaatccgga tggcagcgcc ggggcttcgt cgggcggcac
    29461 gtcgcagtcg caggtgactc cggtcgacct gaccaagggt gcgggtggtg atgcggcgaa
    29521 tgcggccaac cgcatcgtct acttcgactt cgacagttat gtcgtgcgtg acgacgcgcg
    29581 tccggtgatc gatctgaatg ccaaggcgct ggccgccaac aaggcgaaga agatgtctgc
    29641 cgaagggcac accgacgagc gcggcggtcg cgaatacaac ctcgcactcg gccagaagcg
    29701 tgctgaagcg gtggtgcgcg cactgacgtt ggcgggcgcc gccgacggcc aggtcgaggc
    29761 ggtcagcttc ggcaaggagc gtccggccgt cgtgggttcg gacgaagcgg cctacgcgaa
    29821 gaaccgccgc gtcgagctgg tcagccgctg aggtcgccgg catgagtcgg ccgaagccac
    29881 tgccgcatcg tttctggttt tctgcgtgcc tgctgatggc gctgtcggcg ccggcagcgc
    29941 acgccgggct gttcgatgac gacgaggcgc gccgcgcgat tctcgatctg cgccagaaac
    30001 tggagcaggg caacgaggcg cagcgtgcgc gtcaggccga gctcaacgcg cagatgaccg
    30061 atcagatcgg gcagttgcgc agcagcttgc tcgacctgag caaccagctc gaactgatgc
    30121 gcaccgagaa cgcgcgcctg cgcggtcagg tcgagcagct ggcgcgcgaa gcgagcgagt
    30181 tgcaggtcca gatgaaggac ctcaaggagt tgatgcaggg tctcgatgac cgcacgcgtc
    30241 gcctggagcc ccagaaggca tcggtagacg gcaaggactt catggccgac gccgaggaga
    30301 tccgtcagta cgaggccgcg atgacgctgg tgcgcagcgg cgatttcgcg ggcgctgcaa
    30361 cggcgctggc ggtgtttcgc agccgctacc cggccagcgg atacggccaa tcggtgctgt
    30421 tctggctcgg caacgcgcag tacggcaagc gcgactacct cgaggcgatc tcgtcgtttc
    30481 gcgggtttgc gggaagctat cccggtgatc tgcgtgcacc cgaggcgctg ctggccgtgg
    30541 ccaactgcca gttcgagctg aaggacgtga agtccgcccg caagacccta gacgagttgt
    30601 tcaaggccta ccctgactcc gaagcagcgc aggcaggcag acagcggctg gctgcgctga
    30661 agtgaacgag tcggccgttc tgctgccgcg ttgatcgaac ggttcgcatg accgacgacg
    30721 ccgacctgga gcgccgcttc ggcgggctgc ggcgcctgca tggtgatgcc tcctaccagc
    30781 ggttgcgcgc agctcgcatc gcggtcgtgg gactcggcgg agtgggctcc tgggcggccg
    30841 aggcgctggc gcgcagcggc gtggcagaac tcaccctgat cgacctcgac cacgtgtccg
    30901 agggcaacat caatcgtcag atccaggcca cggggcagac gatcgggcag gcgaaggccc
    30961 aggcgctttg cgaacggctc gcggacatcc acccgggctg cgtcctgcgc ccgatcgagg
    31021 ctttcgtcga cgccgagaac tggcccgcgc tgctgacgca cgagatcgac gtgctcgtcg
    31081 atgcgtgtga tcaggttcgc gcgaaggcca cgctcgctgc ctgggcgctg gccacccgaa
    31141 ccgcttttgt cacggt
//
LOCUS       DAOORV010000025        29972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1781_length_29972_cov_6.954240, whole genome shotgun sequence.
ACCESSION   DAOORV010000025 DAOORV010000000
VERSION     DAOORV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29972
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1781_length_29972_cov_6.954240"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1221
                     /locus_tag="PKV56_05290"
     CDS             199..1221
                     /locus_tag="PKV56_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX67261.1"
                     /translation="MKSLRSTLLSVTAVVAGLALSQGALAQSTWTLKTTAVSGGACTQ
                     GAGNSGTYNNSWSCTGATASGKTPAGTGGPLSLSAWSTDRGAGKYATAGGATYTTSGG
                     GNIIYNGSGGGRTEQKLTGTGYASAYLSQQGSSGFGAGSRDEGVGASSPNHAFDSTAP
                     GTQDVLLLSFASSVILDQIGIGWQGNDADITLMRWTGTTAPDRNLGAPAVGGEENLSS
                     AIYNSATPNIAGWELVGSYSNLANGTPRSTGADLEKGSSWWLISTFNTALNGGNTNCK
                     KADGSATTCSQGNDAFKLNFVATLNPVLPPPPPGGEVPEPGSMALAGIALAAVFGARK
                     RKAQQA"
     gene            1439..2392
                     /locus_tag="PKV56_05295"
     CDS             1439..2392
                     /locus_tag="PKV56_05295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX67262.1"
                     /translation="MSISSWTLDRSRLEHVACPLCHGLEFEKLAATDRYNMGIVTMGC
                     LGCGLVMTNPQPTSEALDDFYAHHYRRYYRQSMAPTLSHIRALRIDERTSAAVDFLIA
                     RGDLLPGMAVLDIGASEGSMLRAVGERLPSTRRFAIEPDARLGDFTVQHAGCQVHASL
                     QAFRESQPEPINLVIMNHVLEHIRNPIQFLSSLKPLLASNARIYIDVPDLREYHKLSA
                     LHIAHLYHFGPDTLAQAAASAGYSVQVIEKHSPPRHPRSLRCVLSAAEPATVPAPVNQ
                     RTGWDVVRSCAHHAWYAHGKRWLRSTAAPLLVFGRSQHRAP"
     gene            complement(2399..3040)
                     /locus_tag="PKV56_05300"
     CDS             complement(2399..3040)
                     /locus_tag="PKV56_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOX67263.1"
                     /translation="MAPEPHAQHVLHVWRHPKAIGAAGRCIGRTDLPVDPRKAKRLAH
                     RIRCQARRQGLLHEVCTSPLRRAADVGRWLARWGWRHRIDASLAEIDFGAWDGSNWDE
                     IGESAVADWCADFARYTGHGGESVSMLFERCTGWSEDASRASACCVVGHAGWITALRM
                     LQEGRGAPDSASDWPLPLPLGSRLEIRLEPRAAATSVDQRQQMRSVALDVRAC"
     gene            complement(3030..3899)
                     /locus_tag="PKV56_05305"
     CDS             complement(3030..3899)
                     /locus_tag="PKV56_05305"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOX67264.1"
                     /translation="MRPDEPTVSAAPEVPIAVPPPRGLVPRLLHEARLLFIALQFFTR
                     VPIPRWVGFEPDWLHQSARHFPAMGLLVGAFGALVLWGASRVLPLPVAVLLSMAATML
                     ATGGFHEDGWADTCDGLGGAVTRERALEIMKDSRIGAYGAMGLATMLALKATTLSSLP
                     VAWGCAALLLGHTVSRCASTALIRFLPYAGDIEHAKAKPLAQRLSNSSLAVACGWVLP
                     VATALALWQPLWIVPVIGSAVFAALGACWCGRWFRRRLGGVTGDTLGAAQQITELLVL
                     LSWAAAWGGVRGA"
     gene            complement(3896..4969)
                     /gene="cobT"
                     /locus_tag="PKV56_05310"
     CDS             complement(3896..4969)
                     /gene="cobT"
                     /locus_tag="PKV56_05310"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOX67265.1"
                     /translation="MNSPDEILDRDLATLRARLQLRLDRKTKPMGSLGRIESLALQIG
                     LILRSERPALEQPQLVVFAGDHGLAARGVSAYPSDVTWQMVENFLSGGAAVSVLARQH
                     GLALTVVDAGVRHDFTPRPGLLDRKIAGGTADALDGPAMSEEQCVQALCAGREVVRDL
                     PGNALLLGEMGIGNTSSAALLMSRLAGEPIERCIGRGTGLDDSGLQRKLRVLRGVLHK
                     HPRAVEPLQALSAFGGFEIAMLVGAVLQAVEERRVVVVDGFITGAAVLVAQQMQPAVV
                     QGCVFAHRSDEAGHALMLQGLGATPLLDLGLRLGEGSGAALAWPLLDSAVRLLSEMAS
                     FETAGVATGSDGTSAPPGGGAMA"
     gene            complement(5014..5829)
                     /locus_tag="PKV56_05315"
     CDS             complement(5014..5829)
                     /locus_tag="PKV56_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67266.1"
                     /translation="MATGSEPSTFSTPPPPVAALRVDGLHVQLGGLPVLRGLSLALHP
                     GWTAIVGPNGGGKSTLLRALAGLLPPHAGQVWLEGRPLAAYSARSRGQRIAWLAQQEA
                     GEARGELTVRDVVQLGRLPHLGLFAAPGPLDDAAVDAALATTECTDWQCRRLHELSGG
                     ERQRVLLARALAVEAPVLLLDEPTTHLDPPHQVAVVRLLKRLAKAGTTVVSVLHDLPL
                     ALQADRLVVIQDGRVRADGSPRSTEVQGSLVTVFGGALSIERVGGRLTAVPRL"
     gene            complement(5835..6887)
                     /locus_tag="PKV56_05320"
     CDS             complement(5835..6887)
                     /locus_tag="PKV56_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOX67267.1"
                     /translation="MTDRSRAGLGNATRRATDLGALRRRRFSIGLLLVSIVLAGAGLA
                     SGSDGWSWPWFGDADASLILGQIRAPRTLGAWLTGALLGLAGAVAQGLFRNPLADPYL
                     LGSAAGASLAVVLVLAAGSLGGVAIGWAAAEGLARLGLVGAAFAGAFVGVALTLALAR
                     GAQQTPRLLLAGVIVGVVLSALGDMVSTAAPEALRGKQAFMLGTTGFLGWESVGVLAL
                     GLALTVPIAWRLARVLDALALGEDSARSLGLALNRWRLVLVAVLALATGLAVSQAGLV
                     AFVGLAAPHLVRRCAPAPNGYTLLASAAAGGTLLLAADVLARSLIAPQELPVGVVTAV
                     LGGSYLLWLMRRRRLG"
     gene            complement(6884..7819)
                     /locus_tag="PKV56_05325"
     CDS             complement(6884..7819)
                     /locus_tag="PKV56_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOX67268.1"
                     /translation="MNGNRIGFARSALERGRWALLGLCLYAATGSLQAQTDARAAPVS
                     LIDDRGRQLSLAVPPRRIVSLLPSLTEGVCALGGCERLVGTDRYSNSPARVLALPKLG
                     GMEDAQLERIVALKPDVVLAAASMRAIDRLESLGLKVVVLESKTHADVQRSLQVLATM
                     LGTPDAAAPLWSRIERDVERAHARVPAPMRGQRVYFEVDATPYAAGESSFIGQTLRRL
                     GLANIVPAQDGPFPQLNPEFVVRARPDIVMAVQRNLDEMPSRPGWARLPAMRDGRVCG
                     FSSERFEVLERPGPRLGEAALMLADCLVALAESSR"
     gene            complement(7816..8373)
                     /locus_tag="PKV56_05330"
     CDS             complement(7816..8373)
                     /locus_tag="PKV56_05330"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOX67269.1"
                     /translation="MHELILGGQRSGKSRCAEQRAAAWLTDRTHTAVLLATARAGDEE
                     MRLRIAHHRLERAERVPRLETEEVPLDLAQAVFRHSAPGRLVIVDCLTMWLTNLLMPM
                     EGEPLDDIEWDLTCEAWCDAIGAACGPLLLVSNEIGFGVSPMSREARGFVDRLGRLHQ
                     AVAARCDRVTLMVAGIEIPVKRTPH"
     gene            complement(8416..10248)
                     /locus_tag="PKV56_05335"
     CDS             complement(8416..10248)
                     /locus_tag="PKV56_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX67270.1"
                     /translation="MACLSGSVVQAQTRLPPVVIIATREPVPLDQVTADVVVIDAQRI
                     RESSADSVEDLLRREAGVQLSRTGGPGHSAGIFIRGASSQSTLVLVDGVRIGSATLGQ
                     VSFDAIGLAQIERIEVLRGPGSSLYGADAVGGVVRITTRRGEGPASFSGHVAAGEYGS
                     KEGDVSIGGSGGGFDYTASFSRESSDGVSSLRPGDQFGNYNADRDGYSRRTAQIRLGY
                     SVAPGHRLALSLVDARLRSQYDTSEFAPPDFLQDPSPNFGTRLDTQVVALSYDGVISP
                     LWTTRLQLAHNADESLEGGNFPDRFITRRDQYTWQNAFALDARQQLVAVLERLEERAK
                     ASAFGSEERRHNDSLGLGYSGKFGSHLLSADVRRDDNSVYGGKTTGRLGWGYEIGHGL
                     TARALAGTTFRAPSFNDLFYTGYGVDTVRPERGRSVEFGLSWLSGDSTASVTVYRNRV
                     RDLIAYEPDRAFCPADPSYDFGCARNINRARLQGATVTGGHRLGALSLRATIDLLNAK
                     DDRTGERLDRRARHQETISADYQIEAWTFGATLITLGNRPDGGRRLGGYSTLDLQARW
                     RIASQWQLEAKVLNVADRDVEPARDYRALGRQGWIGLRFSGIGL"
     regulatory      complement(10399..10642)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10689..10943
                     /locus_tag="PKV56_05340"
     CDS             10689..10943
                     /locus_tag="PKV56_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOX67271.1"
                     /translation="MSKIEDLADRVERLLLRHDELQRTNALLEQQLASVTSERDSLRS
                     RLSAARARIDTLIERLPADAPSASGDADLISASPANESAP"
     gene            10940..11245
                     /locus_tag="PKV56_05345"
     CDS             10940..11245
                     /locus_tag="PKV56_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOX67272.1"
                     /translation="MKQIEVTILGQSYVLGCPEGGEALLQASVKAVDREMTTIRTAGK
                     VKARERIAVLAALNLAYRLVQGSDAAPDGEPASPAAVDMNALISRIDKALGEHGQLL"
     gene            11307..11490
                     /gene="ssrS"
                     /locus_tag="PKV56_05350"
     ncRNA           11307..11490
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKV56_05350"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11524..12510)
                     /locus_tag="PKV56_05355"
     CDS             complement(11524..12510)
                     /locus_tag="PKV56_05355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX67273.1"
                     /translation="MTDLELSSGGWGEVLAAWLAGADAALGIKNPVTGRYVHACPKLA
                     GIFNRAQAEVLGSTDAELLGIDLAAPLRAADATAVTLGSSHTTEHRIERDRLRRDFSV
                     TRVLISNADASARFLCLIWTEITSVRQREAQLSLALRQLEQQTAASEALRRETQDAAS
                     RDIVTGLVQNVHFEDQLRREVDLSSREHREFSLVSISIDPAVGVAQSLGDSARLRVVE
                     ALGRLLRSNTRAMDASCRINDDRFAVLLSGVGLATAHSRMEGLRRQCATQIVVQDGQD
                     FGFTVAMGVASFPHTAHTLEQLLKAADSALVAAQARGGNNVALASIRFDTSS"
     gene            complement(12756..13259)
                     /locus_tag="PKV56_05360"
     CDS             complement(12756..13259)
                     /locus_tag="PKV56_05360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOX67274.1"
                     /translation="MNSSILKIAAASLIVAASHCHAEGAISFSRDITIDRSPATVWKY
                     VGDFNAVDLWHPAVRNSTLKGNSTKPGAIRTLKLADGSELVEKLLAYNAAKGSYTYTT
                     LKSPFPIKNYVSTISVSPTADGKTLMTWSATFDAVGVDEDIATEWVGVAFDGGLGKVS
                     ANFEKPQ"
     gene            complement(13276..13776)
                     /locus_tag="PKV56_05365"
     CDS             complement(13276..13776)
                     /locus_tag="PKV56_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOX67275.1"
                     /translation="MKTLALKVTVASLLLATGSCFAAGALSVSREVTVDNSQATVWKL
                     VGNFNALDVWHPAVIKSELKGSATKVGARRLLTLGDGATILESLLTYNAAKTTYSYSI
                     VKSPLPIANYKATITLTPVDETHTLMQWSSTFDANGVSDEQASTIIGGIYDAGMAKVV
                     ANFKKQ"
     gene            14039..14992
                     /gene="ybiB"
                     /locus_tag="PKV56_05370"
     CDS             14039..14992
                     /gene="ybiB"
                     /locus_tag="PKV56_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HOX67276.1"
                     /translation="MSIAPYIKEIGRGKEGARSLTAEQAHDLMSQVLDGRASDLEVGA
                     FTMAMRIKGESADELSGFLAAVHERCIPVSPDGAAVILPSYNGARKLPNLTALLALLL
                     AQEGVPVLVHGPSSDPTRVTTGSIFHDLGLPTARDAQDVRNAWARREPVFMSIETLCP
                     SLARLLDVRWVTGLRNPGHTVAKLLDPTVGSRSLRVVNHTHPEYGASLATFLQRSHAN
                     AILLRGTEGEPVADPRRLPRLDVFLQGELRADLSIAAQEGVLTELPVLPRTFDAATTA
                     VYIQSVVSGEKPAPGPLSRQADCIRSALQTFVKGEAQELIA"
     gene            complement(15096..15878)
                     /locus_tag="PKV56_05375"
     CDS             complement(15096..15878)
                     /locus_tag="PKV56_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX67277.1"
                     /translation="MKTPQVRSIASRDNPLLVRLRKLGRDPVAYRKLGELWIEGDHLC
                     RAFLQRGGVPLQAVISEEGWASPLRDLALQAPVVSVLPAVLLSSVSALESPTPMGFVV
                     PWVRVTEVHPDAASIVLDRLQDAGNVGTILRSASAFGFSQVLALKGTAGLWSPKVLRA
                     GMGAHFGLSLVEGVEPADLHALASPMLATSSHASQAIHEVALPWPCVWVLGHEGEGVS
                     AALQSQCSLTLRIAQPGGEESLNVGAATAICLYESARQRLGR"
     gene            complement(15875..16501)
                     /gene="rnhB"
                     /locus_tag="PKV56_05380"
     CDS             complement(15875..16501)
                     /gene="rnhB"
                     /locus_tag="PKV56_05380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOX67278.1"
                     /translation="MRSRKSLLAEQLGLGWDSPGLMAGVDEAGRGPLAGPVVAAAVIL
                     DELQPIRGLNDSKKLSARTRDRLFDEIRARALCFCIAEASVEEIDRLNILQATLLAMR
                     RAVDGLRLRPQRVLVDGNRLPVLPMPAEAIVQGDAKVQAISAASILAKVHRDRLCIAL
                     HECHPQYGFDGHKGYPTAAHLAALREHGACEAHRRSFAPVRAVLGGSA"
     gene            complement(16473..17591)
                     /gene="lpxB"
                     /locus_tag="PKV56_05385"
     CDS             complement(16473..17591)
                     /gene="lpxB"
                     /locus_tag="PKV56_05385"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOX67279.1"
                     /translation="MVAGEASGDLLAGLLLGGLTRRWPGLASMGIGGPKMVDQGFEAW
                     WPSDKLAVRGYAEVLRHYPELLRIRHRLTQRLLESPPDVFIGVDAPDFNLDLEARLKS
                     HQVRTVHFISPSIWAWRGERIEKIKRAVDHVLCIFPFEPEIYARHGIAATYVGHPLAD
                     TIPLEVPRAASRRALGLDAGERVVALLPGSRRSEIQYIAPTLLEAAREMKRRRPELRF
                     VLPAAPGLRPMIETLLAEHAGGVGIRVLDGCSHEALAACDVTLIASGTATLEAALFKR
                     PMVIAYKMHWLSAQIMRRMNYQPWVGLPNILLQDFAVPEFLQGAATPVNLADAAFAWL
                     DDPAGCQALAARFDALHHTLRRNTAQAATDAIEKILAR"
     gene            complement(17652..18440)
                     /gene="lpxA"
                     /locus_tag="PKV56_05390"
     CDS             complement(17652..18440)
                     /gene="lpxA"
                     /locus_tag="PKV56_05390"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOX67280.1"
                     /translation="MSLIHPTAIIEPGAELHASVSVGPFSIVRERVRIGAGTTIGPHC
                     VIEGRTTIGCDNRIFQFCSLGAVPQDKKYRDEPTELRIGNRNTIREFCTFNIGTSQDA
                     GITSVGDDNWIMAYVHVAHDCQVGNQAILANNATLAGHVHLGDWVIVGGLTGIHQFVK
                     VGPHAMVGFASAVSQDVPPFMMVDGNPLAVRGFNAEGLRRRGFGPERLAAVKQMHRLL
                     YRQGNTLEQARAAIADLAGSTPEAAVDVGVMSEFLARSQRGIVR"
     gene            complement(18475..18921)
                     /gene="fabZ"
                     /locus_tag="PKV56_05395"
     CDS             complement(18475..18921)
                     /gene="fabZ"
                     /locus_tag="PKV56_05395"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOX67281.1"
                     /translation="MTMDIHEILKKIPHRYPILLVDRVLELKKNDFIRALKNVSINEP
                     YFSGHFPQRPVMPGVLMIEALAQAAAILGFETLDISMEENALFYFAGIDGARFKRPVE
                     PGDQLTLEVKLDRQKSGMFKFKARGTVDGELAVEADLMCTVVRKAI"
     gene            complement(18918..19973)
                     /gene="lpxD"
                     /locus_tag="PKV56_05400"
     CDS             complement(18918..19973)
                     /gene="lpxD"
                     /locus_tag="PKV56_05400"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOX67282.1"
                     /translation="MPSDSDPRFALGDLAAQLGAELIGDPARIISRISPLDSATPDAI
                     SFLSNPRYLSQLADTKAGCVIVSAVHRDTAVQRGDALIARDPYLCFAKLTQLWAARLR
                     DSGACRLHPSAVVDPLARLGRDVSIGALAVIEQNAVIGDGAVIGAQCFVGAGASVGSL
                     TRLSPQVVLGARCHIGARGVVHSGAVIGADGFGFAPNAGRWEKIEQLGGVRIGDDVEI
                     GANTCIDRGALEDTVIEDGVKLDNLVQIAHNVHVGAHTAMAATAAVSGSTHIGAHCTL
                     AGGAGVAGHVTIADHVHIGPRSSATRSLLKPGLYTGLFPIDDNASWEKNAATLRQLHQ
                     LRDRIRALEKNNEIKPS"
     gene            complement(19963..20475)
                     /locus_tag="PKV56_05405"
     CDS             complement(19963..20475)
                     /locus_tag="PKV56_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOX67283.1"
                     /translation="MNRLLKSALAASVVLAFTCSAHAEEVKIGYVNSDRVLREAIPAK
                     TAQAKLEAEFAKRDKDMADLAGKLKANSEKLEKEAPTLSESERSRRQRDLVEQDREFQ
                     RKRREFQEDLTQRKNEELSAVVDRANKVIKQIFESEKYDLIIQEAVFAGPKIDITDKV
                     VKALNAQGAK"
     gene            complement(20475..22790)
                     /gene="bamA"
                     /locus_tag="PKV56_05410"
     CDS             complement(20475..22790)
                     /gene="bamA"
                     /locus_tag="PKV56_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOX67284.1"
                     /translation="MRPTLRFPSSLTSFRPSLICLALATALQAGSAWAVEPFKLVDIR
                     VEGLQRSDAGTVFASLPFRIGDTYTDEKGVAALRALFATGLFKDVRIQIDGSVVVVIV
                     DERSVIGSIDFVGAKEFEKDALVKALKDFGVGEGQPFDKALADRAEQELKRQYLSRSL
                     YGVEVVTTVTPMERNRVNVTFNISEGSAAKIKELRILGNTAFSESSLKDLLELNDGGW
                     LTWYTKANRYSRAKLNADLETLRAYYLNRGYLEFNVESTQVAISPDKQDITITINVNE
                     GQAYTVTSVKLEGEYLSKEDDFKSLVTIKPGEPYRAEAVAETTKAFTERFGTFGYAFA
                     RIEARPEIDRVNGRVDLTLVANPQRRVYVRRISVAGNARTRDEVVRREFRQFESSWYD
                     GNRIKLSRDRVDRLGYFSDVNIDTNEVPGTSDQVDLILTIKEKPTGNLLLGAGFSSAD
                     KISLTASVKQENVFGTGNYLGLEFNTSKSQRTIVLSTIDPYFTVDGISRAFDLYYRTS
                     SPLNSQGEDYKLVTPGGSVRFGVPFSEYDTVFFGIGYERTEIQGTNNLPDSYFRYREQ
                     FGSTSSSVPLTIGWTRDQRDSALVPTRGRFQRVNVDLGLLGDQRYMRANLQFQEYFNP
                     FQRVTLGLNAEFGYGKGLGGRPYPIFKNFFGGGLGTVRGFDQGSLGGVDNLGAYLGGN
                     RRLNINSELYLPLPGTGNDRTLRWFAYTDVGNVWAVSDKMTVDSLRASVGLGVSWVSP
                     VGPLKVSYGTPIKKEPTDRIQRLQFQLGTAF"
     gene            complement(22799..24169)
                     /gene="rseP"
                     /locus_tag="PKV56_05415"
     CDS             complement(22799..24169)
                     /gene="rseP"
                     /locus_tag="PKV56_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOX67285.1"
                     /translation="MITTVLAFALTLGLLIVIHEYGHYRVAVACGVKVLRFSVGFGRV
                     VWRHQSHPDSTEFVLSVLPLGGYVRMLDEREGPVAASELGRAFTRQTLWKRSAIVAAG
                     PVANLLLAVVLYAAAHWIGMDEPKAVLAAPLAQSVAADAGLQSGDWVQATSTDGEDWN
                     EVRSMTDFVWRVTQAVLENESLHLRVSDARGQHERSVLLPLERLEGRELDAQLIRRIG
                     LGGVYTEPVMGEVKPAGPAATGGVRAGDRVLSVDGDVIPDAASLRERIRASATASDPA
                     PQQWRVERAGQAIDLVVQPVALIDGERRIGRIEAFIGGPPEMVMVSYGFIDGLSRAIT
                     RTWNTSWLTLKMFGRMVIGEASLKNLTGPLTIADYAGQSVRVGFAYYLGFLAVVSISL
                     GVLNLLPLPMLDGGHLMYHLFEAMTGRPVPDLWLERLQRGGVAIMLTLMSIALFNDVA
                     RLMGLH"
     gene            complement(24166..25371)
                     /gene="ispC"
                     /locus_tag="PKV56_05420"
     CDS             complement(24166..25371)
                     /gene="ispC"
                     /locus_tag="PKV56_05420"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOX67286.1"
                     /translation="MSNANAGSRLRICILGSTGSIGTSTLDVLSRHPERFEVFALTAH
                     HRVDELLAQCIAWRPKFAVLPESGPAQTLQAQLRNAGSETAVLVGPQALCEMAAHPEV
                     DAVMAAIVGAAGLPPCLAAARAGKRLMLANKEALVVGGALFMSAVREGGAHLLPIDSE
                     HSAIFQCLPEDRSAWGARIDHIILTASGGPFRTRDLNTLSSVTPDQACAHPNWVMGRK
                     ISVDSATMMNKALEVIEARWLFDLTPEQIKVVIHPQSIVHSMVVCRDHSVLAQMGTPD
                     MRVPIAYGLSFPERMTSGAGLLDFPSLSALTFEPADFQRYPGLPLAWETLRGPEGSTA
                     VLNAANEVAVAAFLAGAIGFEGIHQVNARTLEAVLPEHDECSSVEGLIGLDARVRRAA
                     SSQVERLSR"
     gene            complement(25373..26209)
                     /locus_tag="PKV56_05425"
     CDS             complement(25373..26209)
                     /locus_tag="PKV56_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOX67287.1"
                     /translation="MLKQRVITAVVLLAIVLPALFAPVPLPFAALTLVFIAAAGWEWV
                     RLNGGRSSSSIGSALLLGGACALALGAGWEHSAPPSTWWAAMGVWVLGGSWALKAGPA
                     GWGLLPAGLRWPLGLIGLWLAWLALASARTQGINFILSIFSLVWVADIAAYFGGRAFG
                     KRKLALAISPGKSWEGVWSGMIGVLLLAVLWMQADRTWVLGSPSLFTQLHAQHGVVGL
                     VLVCIFLTAMGVVGDLVESLVKRQAGAKDSSGLLPGHGGVLDRVDALLPVFPLALALS
                     SV"
     gene            complement(26212..26946)
                     /gene="uppS"
                     /locus_tag="PKV56_05430"
     CDS             complement(26212..26946)
                     /gene="uppS"
                     /locus_tag="PKV56_05430"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOX67288.1"
                     /translation="MPNASKVPRHIAIVMDGNGRWAKSRFKPRFLGHKQGVDTLVNTV
                     LACADRGVEYLTVFAFSSENWKRPNDEVSGLMSLVLLAVSKYLGVMHKNGVRVRIVGD
                     RTDIAEHVREAWDHAESSTRNNTRITLSVAFNYGGRWDVVQACQHAMRDGVRADDLDE
                     ATLSRYMALSHAPDPDLFIRTGGEVRISNFLLWQAAYSELCFSDCLWPEFGEAELDAA
                     LADYARRDRRFGGIEEATSAELPLVR"
     gene            complement(27004..27564)
                     /gene="frr"
                     /locus_tag="PKV56_05435"
     CDS             complement(27004..27564)
                     /gene="frr"
                     /locus_tag="PKV56_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOX67289.1"
                     /translation="MSIAEIRKNAEQKMGRSVEAFKTELQKIRTGRAHPGILDQVHVD
                     YYGSPVPISQVANVSLLDARTISVQPWEKGMGAKIEKAIRESDLGLNPSSQGDLIRVP
                     MPALSEERRKDLTKIVRNAGEDARIAVRNLRRDANDHAKRLLKDKEISEDDERRSLDE
                     MQKLTDRTIAEIDRLVAAKEAEVMAV"
     gene            complement(27589..28299)
                     /gene="pyrH"
                     /locus_tag="PKV56_05440"
     CDS             complement(27589..28299)
                     /gene="pyrH"
                     /locus_tag="PKV56_05440"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOX67290.1"
                     /translation="MPAYKRILLKLSGEALMGDDAYGINRATIVRMVREIQEVTLMGC
                     EVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALADTMRQEGMTARVMSA
                     IAIDQVVEPYVRPKALQYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAQIVLKAT
                     KVDGVYTADPKKDPNATRYSQISFDEAINRNLQVLDATAFALCRDQKLPIKVFSIFKP
                     GALKRVVQGEDEGTLVHV"
     gene            complement(28390..29307)
                     /gene="tsf"
                     /locus_tag="PKV56_05445"
     CDS             complement(28390..29307)
                     /gene="tsf"
                     /locus_tag="PKV56_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOX67291.1"
                     /translation="MTAITASMVGELRAKTDAPMMECKKALTEAGGDMERAEELLRVK
                     LGSKAGKAASRVTAEGVIVSAVVGQAGALVEVNCETDFTSKNDMFLAMANACARLVAE
                     NNPADVAALSALPHSQDGYGPTLEDVRKGLIGTIGENMSIRRFKRYEGAKLASYLHGT
                     RIGVMVEFEGDDVAAKDVAMHVAAMKPVALQSSDVPAKLIETERSVATQKAAEDAEKA
                     KAEGKTPQSAEIVAKRIDGSVAKFLKEVSLFNQAFVKNDKQTVEQMLKDKGTVVKGFT
                     LFVVGEGIEKKVDDFAAEVAAQVAAAKGA"
     gene            complement(29429..>29972)
                     /gene="rpsB"
                     /locus_tag="PKV56_05450"
     CDS             complement(29429..>29972)
                     /gene="rpsB"
                     /locus_tag="PKV56_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOX67292.1"
                     /translation="VGTKRQAREVVALEAARAGMPYVDQRWLGGMLTNFKTVKGSLKR
                     LKDMQAQVESGTEIRIKKEALMFQRELAKLEKDIGGIQDMNALPDALFVIDVGYHKIA
                     IAEANKLGIPVVGVVDSNHSPIGIDYVIPGNDDSAKAVALYAKAVADAVLEGKANSTN
                     EVVQAVAAEGDEFVEVSEAH"
BASE COUNT         5550 a        10014 c         9325 g         5083 t
ORIGIN      
        1 agttccgggc gccccaccga ggcacgagag gaactggaag ccgcactgaa gggcaaccgg
       61 caattcgaga acgccaagcc agcggaacga ctgttgagca cccttaagtg aggggtgcaa
      121 cgaatctcca cgctacttac acttggacat atgtcggttg aagaccgcaa tccgattttt
      181 ctaaggatca gaaaccccat gaaaagccta cgctcaaccc ttctttcggt aacagccgtc
      241 gtggccgggc tggccctgtc gcagggtgcc cttgcgcagt ccacatggac cctcaagacc
      301 accgcagtca gcggaggagc ctgtacccag ggtgcaggta acagtggcac ctacaacaac
      361 tcctggagtt gtaccggcgc taccgcctct ggcaagacac ctgccgggac cggtgggcct
      421 ctctcgcttt cggcttggtc gacggatcga ggcgctggca agtacgcaac cgcaggtggc
      481 gccacttaca cgaccagcgg cggcggaaac atcatttaca acggttcagg cggcggtagg
      541 acagagcaga aattgaccgg tactggttac gccagcgcgt atctgtccca gcagggaagt
      601 tcagggttcg gcgctggcag tcgtgacgag ggcgtcggag caagcagccc gaaccatgct
      661 ttcgattcca cagcaccggg cacgcaggac gtgttgttgc tgtcctttgc ctcgagcgtg
      721 attctcgatc agatcggtat cggctggcag gggaacgatg cagacatcac tttgatgcgc
      781 tggaccggca caactgcgcc ggaccgaaac ttgggtgccc ctgccgtcgg gggtgaagag
      841 aatctctcga gcgcgatcta caacagtgcg acacccaaca ttgcaggctg ggagctcgtg
      901 ggcagctact ccaatctggc gaatggcacg ccaaggtcta ccggcgcaga cttggaaaag
      961 ggttcgagct ggtggctgat cagcacgttc aacacagcct tgaacggtgg caacacgaac
     1021 tgcaagaagg ccgatggcag cgccaccacc tgctcccaag gcaacgacgc attcaagctc
     1081 aactttgtcg cgaccttgaa cccggtgttg ccgccgccgc ctccgggagg agaggttccc
     1141 gagcccggct ccatggctct ggcgggtatc gcactggccg cagtctttgg cgcccgcaag
     1201 cgcaaggcgc agcaggcctg aacgatccgt cgatcacgtg aagtcccagc gcccaggcgc
     1261 tgggactttt tcatttgtgc atcggcgatt gcggtcatgc gctgtgtcgc actcccttca
     1321 acccgggttt cattccattc gggcagctgc actcggcaac aacctcgtct gttcgggagg
     1381 tataaacacg caaaggtcgg atcgctcgcc gatcgagcct gccaaccttc ttccttctat
     1441 gtcgatttca tcctggacac tcgatcgcag ccgtctcgaa catgtcgcat gcccgctgtg
     1501 tcatggcctg gaattcgaga agctcgctgc cacggaccgc tacaacatgg gcatcgtcac
     1561 gatgggttgc ctgggctgcg ggctggtgat gacgaacccg cagccgactt cggaagctct
     1621 ggacgacttc tacgctcacc actaccgccg ctactaccgg caaagcatgg cgccgacgtt
     1681 gagccacatc cgtgccctgc ggatcgacga gcgcacctcg gctgccgttg atttcctgat
     1741 tgcgcgaggt gacctgctgc cgggcatggc ggtgctggac atcggggcgt ccgagggctc
     1801 gatgctgcgt gcggtgggag agcggctgcc ctcgacgcgg cgctttgcga tcgagcccga
     1861 cgcgaggctg ggcgatttca ccgtccagca tgcgggctgc caggtgcacg ccagcctgca
     1921 ggcgtttcgt gaatcgcagc ccgagccgat caatctggtc atcatgaatc acgtgctcga
     1981 gcacatcagg aacccgatcc agttcctgtc atcgctgaag ccattgctgg catcgaacgc
     2041 tcgcatttac atcgacgtgc ccgatctgcg cgagtaccac aagctcagcg cactgcacat
     2101 cgcacacctg taccacttcg gccccgacac gctcgctcag gccgccgcca gcgccggcta
     2161 cagcgtgcag gtgatagaga agcattcacc gccccggcat ccgcgatcgc tgcgctgcgt
     2221 gctgtctgcg gcggagccgg caacggttcc tgcaccggtc aaccagcgca cgggctggga
     2281 tgtcgttcga tcgtgcgcgc atcacgcctg gtatgcccac ggcaaacgct ggctgcgcag
     2341 cacagcggcc ccgctgctgg tgttcgggcg cagccagcac cgcgcgccct gaaccagctc
     2401 agcatgccct gacgtcaagc gcgaccgagc gcatttgctg gcgttgatca acagacgtcg
     2461 cggcagcgcg tggctccaga cgaatttcca accgtgaccc caggggcagc ggcagcggcc
     2521 agtcgctcgc gctgtcaggc gcgccgcgac cctcctgaag catgcgcaaa gcagtgatcc
     2581 acccggcgtg ccccaccaca cagcacgccg atgcacggct cgcgtcttct gaccacccgg
     2641 tgcatcgctc gaaaagcatc gacacgcttt cgccgccatg tccggtgtag cgagcgaaat
     2701 ccgcgcacca gtcggcgaca gcggattcgc cgatttcgtc ccagttcgag ccgtcccagg
     2761 cgccgaagtc gatctcggcc agtgacgcat cgattcgatg gcgccagccc cagcgcgcca
     2821 gccagcgtcc gacgtcggca gcgcgcctca gcggcgacgt gcagacctcg tgcagcaggc
     2881 cctgcctgcg cgcctggcaa cggatgcggt gggccagccg tttggccttg cgcgggtcga
     2941 cgggcagatc ggtgcgaccg atacagcgcc cggcagcacc gatcgccttc gggtggcgcc
     3001 agacgtgcag cacatgctgc gcgtgcggtt caggcgccac gcacgccgcc ccaagccgca
     3061 gcccacgaca gcagcacaag caattccgtg atctgttgcg cggctccgag cgtgtcgccc
     3121 gtcacaccgc ccaggcgacg gcggaaccag cgcccgcacc agcacgcccc gagcgcggcg
     3181 aacaccgcac tgccgatcac cggcacgatc cacagtggct gccacagcgc gagcgcggtc
     3241 gcgaccggca acacccagcc gcatgcaacg gccagactcg aattcgacag acgctgagcc
     3301 agcggcttgg ccttggcgtg ctcgatgtcg ccggcataag gcaggaagcg gatcaatgcg
     3361 gtcgatgcac atcgcgaaac ggtgtgaccc agcagcaagg cagcgcagcc ccacgcgaca
     3421 ggcaacgacg acagcgtcgt ggccttcagc gccagcatcg tcgccagtcc catggcaccg
     3481 taggcaccga tgcgcgaatc cttcatgatt tccagggcgc gttctcgagt gacggcgcct
     3541 cccaggccat cgcaggtatc ggcccagcca tcctcgtgaa atccgccggt ggccagcatc
     3601 gtggccgcca tcgacaacag cacggcgacc ggcaagggca acacccggct tgccccccag
     3661 agcaccagcg caccgaatgc accgaccagc agacccatcg ctggaaagtg gcgcgcactc
     3721 tggtgcagcc aatcaggctc gaagccgacc cagcgcggaa tcggcacgcg cgtgaagaac
     3781 tgcagggcaa tgaacaacaa acgtgcttcg tgcaggagtc ggggcaccag accgcgcggc
     3841 ggtggcaccg cgatcggcac ttcaggagcg gcagacaccg tcggctcgtc gggtctcatg
     3901 ccatcgcacc gccgcctggc ggcgccgaag tcccatcgga gccggtggcg actccggcag
     3961 tttcgaagct cgccatctcg ctcagcaggc gcaccgcact gtccagcaac ggccacgcca
     4021 gcgcagcccc cgaaccctcc ccgagtcgca ggcccagatc gagcaacggc gtcgcgccca
     4081 gcccctgcag catcaacgca tggccggctt catcggaacg gtgagcgaac acgcagccct
     4141 ggaccacggc tggttgcatc tgctgcgcca ccagaacggc tgcgccagtg atgaatccat
     4201 cgacgacgac gacacgacgc tcctccaccg cctgcagcac agcgccgacg agcatcgcga
     4261 tctcgaagcc gccgaaggca gacaaggcct gcagcggctc gacggcgcgc gggtgcttgt
     4321 gcagcacgcc gcgcagcacc ctcagcttgc gctgcaggcc ggagtcgtcg agcccggtgc
     4381 cccggccgat gcaacgttcg atcggctcgc cggcgagcct cgacatcaac agcgccgcgc
     4441 tcgacgtgtt cccgatcccc atctcgccga gcagcaaggc atttcccggc aggtcacgca
     4501 ccacctcccg accggcgcac agggcctgca cgcactgctc ctccgacatc gccggcccgt
     4561 cgagcgcgtc ggccgtgccg ccggcgatct tgcggtcgag cagccccggc cgcggcgtga
     4621 agtcgtgccg cacgccggcg tccaccacgg tcagtgccag accatgctgc cgcgccagca
     4681 cgctgacagc cgccccaccc gaaaggaaat tctcgaccat ctgccaggtc acgtccgagg
     4741 gatacgcaga gacacctcgc gccgccagac cgtgatcgcc ggcgaagacc acgagttgag
     4801 gctgttccag cgcgggccgt tcactgcgca ggatcaggcc gatctgcagc gccagcgatt
     4861 cgatgcgacc cagcgatccc atcggcttcg tcttgcggtc cagccgaagc tgcagccggg
     4921 cgcgcagggt ggcgaggtct ctatcgagga tttcgtcggg cgagttcatg ctggaatcac
     4981 agggagtcag atccggtaac ggtgatgccc ggctcacagg cgcggaactg cggtcagccg
     5041 cccgccgacc cgctcgatcg acagcgcccc gccaaaaaca gtgacaagac ttccctgcac
     5101 ctcagtcgat cggggcgacc catcggcacg cacgcggccg tcctgaatca ccaccagccg
     5161 gtcggcctgc agcgcgagcg gcaggtcatg cagcacgctg accaccgtcg tgcccgcctt
     5221 tgccagacgc ttcagcaggc gcaccaccgc cacctgatgt ggcgggtcga ggtgtgtcgt
     5281 cggctcgtcc agcagcagca cgggtgcctc gaccgccagg gcgcgtgcca gcagcacgcg
     5341 ctggcgctct ccgccagaga gctcgtgcag ccgtcgacac tgccaatcgg tgcactcggt
     5401 ggtggcgagc gccgcatcga ccgccgcatc gtccagcggc cccggcgccg cgaaaaggcc
     5461 caggtgcggc aggcgaccca gctgcaccac gtcgcgcacg gtcaactcgc cacgggcctc
     5521 gcctgcctct tgctgggcca gccaggcgat gcgctggccg cgcgagcggg cgctgtaggc
     5581 agcaagtgga cggccttcca gccagacctg tcccgcgtgc ggcggcagca ggccggccaa
     5641 agcgcgcagc agggtcgatt tgccacctcc gttgggcccc acgatggcgg tccagccggg
     5701 gtgcaaggcg agcgacagcc cgcgcaagac cggcagcccg cccagctgca cgtgcagacc
     5761 gtcgacacgc agcgccgcga ccggcggcgg tggcgtcgag aaagtggagg gttccgatcc
     5821 ggtggccatg cgtgtcaccc cagcctgcgc cgacgcatca gccacagcag atagcttccg
     5881 cccagcacgg cagtcaccac gccgaccggc agttcctggg gcgcgatcag gctgcgcgcg
     5941 agcacgtcgg cggcaagcag cagggtgccg ccggcggcgg cgctggccag cagcgtgtac
     6001 ccgttgggtg cgggcgcgca gcggcgcacc aggtgcggtg ctgcgagacc gacgaaggcg
     6061 accagcccgg cctgcgacac cgccagaccg gtggccagag caagcaccgc caccagcacc
     6121 agacgccagc ggttcagcgc caggcccagg ctgcgtgcgc tgtcctctcc gagcgccagg
     6181 gcgtccagca cgcgagccag gcgccaggca atcggcaccg tcagcgccag cccgagggcc
     6241 agtacaccga cactctccca ccccaggaag ccggtcgtgc ccagcatgaa tgcctgcttg
     6301 ccgcgcaggg cctcgggcgc cgcggtggac accatgtcgc ccagcgccga cagcaccacg
     6361 ccaacgatca cacccgccag cagcaggcgc ggcgtctgct gcgccccgcg cgcgagggcc
     6421 agcgtcagcg cgacaccgac gaacgcacca gcaaaggctg ctccgaccag acccagtctc
     6481 gcgagccctt cggcggctgc ccagccgatc gcaacgccac cgagcgagcc ggccgccagc
     6541 accagcacca ccgccaggga ggcgcctgcc gccgatccga gcagataggg atcggccagc
     6601 ggattgcgga acagcccctg agcgaccgcc ccggccagac ccagcaacgc gccggtgagc
     6661 caggcgccca gcgtgcgtgg cgcacgtatc tggccgagga tcaggctggc atcggcatcg
     6721 ccaaaccacg gccatgacca gccgtcactg cccgacgcca gcccggcccc ggcgagcacg
     6781 atggacacga gcagcaaacc gatcgagaag cggcggcgac gcagcgcacc taggtcggtg
     6841 gcccggcgcg tggcgttgcc gagaccggcc cgcgatcgat cggtcatcgc gatgactccg
     6901 ccagtgccac caggcagtcg gccagcatca gtgccgcttc gcccagtcgc ggtccgggtc
     6961 gctcgagcac ttcgaatcgt tcgctgctga atccgcagac tcggccgtcg cgcattgcag
     7021 gcaaccgcgc ccagccaggc cggctcggca tctcgtcgag attgcgctga acggccatca
     7081 cgatgtcggg cctcgcacgc acgacaaact cggggttgag ctgcggaaag gggccgtcct
     7141 gcgcaggcac gatgttggcg agcccgagac gccgcaaggt ctgaccgatg aacgaggatt
     7201 ccccggcggc atacggcgtc gcgtcaacct cgaaatagac gcgttgcccg cgcatcgggg
     7261 cgggcaccct ggcgtgggcg cgctcgacgt cgcgctcgat gcgcgaccac agcggcgcag
     7321 cggcgtccgg cgtgcccagc atcgtcgcca gcacctgcag cgagcgctgc acatcggcat
     7381 gcgtcttcga ttcgaggacc accaccttga ggcccagcga ttcgagccgg tcgatcgcgc
     7441 gcatcgatgc tgcagccagc acgacatcgg gtttcaatgc cacgatgcgt tcaagctgcg
     7501 cgtcttccat cccgcccagc ttcggcaacg ccagcacccg agccggcgaa ttcgaatagc
     7561 gatcggtgcc gaccagccgc tcgcagccgc cgagcgcgca gaccccttca gtcagggagg
     7621 gcagcaggct cacaatgcgc ctcggtggca cggccaggct cagctgccga ccccggtcgt
     7681 cgatcaggga aacaggcgcc gcccgggcat cggtttgtgc ctgaagcgat ccagtcgcgg
     7741 catacaggca cagtcccagc agcgcccacc ggccacgctc gagcgcgctg cgtgcgaagc
     7801 cgatgcggtt cccgttcaat ggggcgtccg tttcaccggg atctcgatac cggcaaccat
     7861 cagcgtcaca cgatcacagc gggccgcgac cgcctgatgc aggcgcccca ggcgatccac
     7921 gaagccccgc gcttcgcgcg acatcggaga gacgccgaat ccgatctcgt tggaaaccag
     7981 cagcagcggt ccgcatgcag cgccgattgc atcgcaccag gcttcgcacg tcaggtccca
     8041 ttcaatgtcg tcgagcggtt caccctccat tggcatcagc aggttggtca gccacatcgt
     8101 caggcagtcc acgatcacca gccgacccgg cgcactgtga cgaaacaccg cctgggccag
     8161 atccagcgga acttcttcgg tttcaagccg aggcacgcgc tcggcacgtt ccagtcggtg
     8221 gtgcgcgatg cgcaggcgca tctcctcgtc acccgcgcgg gcagtggcca gcagcaccgc
     8281 cgtatgcgtg cgatcggtca gccaagcggc agcgcgctgc tcggcgcaac gcgacttgcc
     8341 gctgcgctgc cctccgagga tcagttcgtg caccgcattt gctccatgcc gaagtgtcgc
     8401 gccgaagcgg tgcgactaca gcccgatgcc gctgaaacgc aggccgatcc agccctggcg
     8461 gcccagcgcg cggtagtcgc gggccggttc gacatcccgg tcggcgacgt tcagcacctt
     8521 ggcctcgagc tgccactgcg atgcaatgcg ccagcgtgcc tgcaggtcga gtgtcgaata
     8581 cccgccaagc cgcctgcctc catcgggccg gttgccgagc gtgatcagcg tggcaccgaa
     8641 ggtccaggcc tcgatctggt aatcggcgct gatcgtttcc tggtgcctgg cgcggcgatc
     8701 cagccgctcg ccggtgcggt cgtccttcgc gttcagcagg tcgatcgtcg ctctcaggct
     8761 gagcgcaccc aggcgatgcc cgccggtgac ggtcgcgcct tgcaggcggg cacgattgat
     8821 gttgcgcgcg cagccgaagt cgtaggacgg atcggcgggg cagaaggcac gatcgggctc
     8881 gtaggcgatc aggtcgcgca cgcgattgcg gtagacggtg accgaagcgg tgctgtcgcc
     8941 cgacagccag ctcagaccga attcgacgct gcggccgcgc tcgggtcgca cggtgtcgac
     9001 gccgtaaccc gtgtagaaaa ggtcgttgaa actcggggcg cgaaacgtgg tgcccgccag
     9061 cgcccgcgcc gtcaatccgt gcccgatctc gtatccccag cccaggcgac ccgtggtctt
     9121 gcccccatag accgagttgt cgtcgcgccg cacatccgct gacagcagat gcgatccgaa
     9181 cttgcccgaa tagcccaggc ccagcgagtc gttgtgccgg cgctcctcgg atccgaaggc
     9241 cgaggccttc gctcgttcct cgagccgttc gagcaccgcg acaagctgct ggcgcgcatc
     9301 cagcgcgaac gcgttctgcc acgtgtactg atcacggcgc gtgatgaagc gatcggggaa
     9361 attgcctccc tccagcgatt cgtccgcgtt gtgcgccagt tgcaggcggg tggtccacag
     9421 cggactgatc accccgtcgt aactgagggc cacgacctgt gtatcgagac gggtgccgaa
     9481 gttgggagac ggatcctgca agaagtcggg cggcgcgaac tcggaggtgt cgtactgcga
     9541 cctcagccgc gcatcgacca ggctgagtgc cagtcgatgc ccgggcgcca cgctgtagcc
     9601 caggcggatt tgagccgtgc ggcgactgta gccatcccgg tcggcgttgt agttgccgaa
     9661 ctggtctccc ggacgcaggg aggaaacgcc gtcgctgctt tcgcggctga acgaagcggt
     9721 gtagtcgaag ccaccacccg agcctccgat cgacacgtcg ccctcctttg aaccgtactc
     9781 gcccgctgcg acatgcccgg agaagctcgc gggcccttcg ccacgccgcg tggtgattcg
     9841 cacgacgcct cccaccgcat cggcgccgta gagactggag cccgggccac gcagcacctc
     9901 gatgcgttcg atctgcgcca ggccgatcgc atcgaacgac acctggccca gcgtcgcgga
     9961 tccgattcgc acgccatcga cgagcaccag cgtgctctgc gaactggcgc cgcggatgaa
    10021 gatgccggcc gagtgccccg gcccgccagt ccgcgacagt tgcacgccag cctcgcggcg
    10081 cagcaggtct tcgaccgaat cggcggacga ctcgcggata cgctgcgcat cgatcacgac
    10141 gacatcggcc gtgacctgat cgagaggcac cggctcgcga gtggcgatga tgaccaccgg
    10201 cggcaggcga gtctgcgcct ggacaacgct gccagaaagg caggccacgg tgatcacgga
    10261 aagaaggacg agcggagatc ggctggaagc agccagtggc gcgcgtgacg ccgagcggaa
    10321 ggaacgcatg atgagaaacc cgacaagatg caggcgaccc gcttccccgc gggcgcatgc
    10381 gatgcagatg cggttcgaga accgcacccc cttgttggcc ggtatccggg ctgatggagc
    10441 aaccttcgcg accttcccag agacaaagtg tctccagtgg ctgtgagcgc gagagcggaa
    10501 accgcggtcg aaaggaaccg gatttcgtcc accgtaccgt tgcgggggca gcgcagtttg
    10561 agcgcctcgc ggcactttct gcttcccgtt taactgcgac gacaagaaca tcgtcgcggg
    10621 caccaaacag ctgtcgattg tatgcaccgc cctacaatga aaagtcactt gtcgaccgag
    10681 gctgtgccat gtcgaagatc gaagacctcg ccgaccgggt cgagcggctg ctgctgcggc
    10741 atgacgaact gcaacgcacc aatgcactgc ttgaacagca gcttgcatcc gtgaccagtg
    10801 aacgggacag cctgcgatca cgcctgagcg cagcacgcgc acgcattgac acactgatcg
    10861 agcggctgcc ggccgacgcc ccttccgcca gtggcgatgc tgatctcatt tctgcttcgc
    10921 ccgccaacga gtccgctcca tgaaacagat cgaagtcacg atcctcggcc agagctatgt
    10981 gctcggatgc ccggaaggcg gcgaggcgtt gttgcaggcc tcggtgaagg ccgttgatcg
    11041 cgagatgaca acgatccgca ccgcaggcaa ggtgaaggcg cgcgagcgca tagcggtgct
    11101 ggcggcactg aacctcgcct acaggctggt gcaaggctcc gatgccgcgc cggatggcga
    11161 gccggcttct cctgccgcgg tcgatatgaa tgccttgatt tcccgcatcg acaaggcgct
    11221 gggcgagcat ggtcagttgt tgtgacgcaa tcggcttcgc ggtcaccctt gggacgccac
    11281 aggtacgtcg tgcaggcgta gactttcttc gtccgttgcg ttcgcgtgag tttttaattc
    11341 cttgaaccga tgctctcgcg agcacgggct gggaacattg ccgagtgggt gtgatcgtct
    11401 cgcgtcagat gaacccgaag cacggttgac ccggcccacc tgaatcccct gggttcagga
    11461 atgcggctca cggctcgcag cggacacccc gattttctcg tgagccaggt cttgcgcaga
    11521 ccttcaggac gaggtgtcga agcggatgct cgccaaggca acgttgtttc cgcctcgcgc
    11581 ctgggcggcc accaacgcac tgtctgccgc cttcagcagt tgctccagcg tatgcgctgt
    11641 atgcggaaag ctcgcaacac ccatcgccac ggtgaatccg aaatcctgac catcctgcac
    11701 gacgatctgc gtggcgcact ggcgcctcag gccctccatc cgcgaatgag cggtggccag
    11761 gcccacacca gacagaagga cagcgaaacg atcgtcattg atgcggcagg atgcatccat
    11821 cgcgcgggtg ttgctgcgca gcaatcggcc cagggcctcc acgactcgca gacgcgcact
    11881 gtcacccaga gactgtgcaa cgccgaccgc aggatcgatg gagatcgaga ccagcgaaaa
    11941 ctcgcggtgc tcgcgagatg acaggtcgac ctcgcgccgc agctgatcct cgaagtgaac
    12001 gttctggacc aggccggtga cgatatcgcg cgaggctgca tcctgcgtct cccgtcgcag
    12061 cgcttcgctg gcggccgtct gctgttcgag ctgtctgagt gcgagcgaca gttgggcctc
    12121 tcgctggcga accgaggtga tctcggtcca gatcaggcac aggaaacgag cactcgcatc
    12181 ggcgtttgaa atcagcacgc gtgtcacgct gaaatctcga cgcaggcggt cgcgctcgat
    12241 tcggtgctcg gtggtgtgcg acgagcccag agtcacggcc gttgcatcgg cggcccgcaa
    12301 cggcgcggcg agatcgatgc ccagcaattc cgcatcggtg gagccgagga cctccgcttg
    12361 cgcccgattg aagatcccgg ccagcttcgg gcaggcatgc acatagcgtc cagtcaccgg
    12421 gttcttgatc cccagggcgg cgtcggcgcc agccagccag gccgcaagca cctcgcccca
    12481 cccaccggaa ctcagttcca ggtcagtcat caactgctgc aacgagactg ccgcttcaac
    12541 gccgccagac tgcaccggct gattcattcc tgccctttca tcaccaccgg cccatcacgg
    12601 gttcgaggac gacccgctct cgcgaagcgg acagcctagc tcaatgctgg ccccatcacc
    12661 gaaaaaaact ggatacgggc ggcctcaatg catgctgccc ctcacttgaa ggggcagcag
    12721 gattggaacg atgcaaaggg aggaatccgg tcgacttact gcggcttttc gaagttggcc
    12781 gacaccttgc ccagcccgcc atcgaacgcc acgccgaccc attcggtcgc gatgtcctca
    12841 tcgactccca cggcatcgaa ggtggcactc caggtcatca gcgtcttgcc gtctgctgtg
    12901 gggctcacgg aaatggtcga gacatagttc ttgatcggga acgggctctt cagcgtggtg
    12961 tatgtgtagc tgcccttggc ggcgttgtaa gccagcagtt tctcgaccag ttcgctaccg
    13021 tcggcgagct tcagcgttcg gatcgcgccc ggcttggtgc tgttgccctt gagggtgctg
    13081 ttgcgcacgg ccggatgcca cagatcgacg gcgttgaagt cgccgacgta cttccagacc
    13141 gtggccggag agcggtcgat cgtgatgtcg cgcgagaagg agatggcacc ttctgcatgg
    13201 caatggctgg cagcaacgat caggctggcc gcggcgattt tgaggatcga tgagttcatg
    13261 ttcggattct cctgatcact gcttcttgaa attggcgacc accttggcca taccggcatc
    13321 gtagatgccg ccgatgattg tcgaggcctg ctcgtcgctg acaccgttgg catcgaatgt
    13381 cgatgaccac tgcatcaggg tgtgcgtctc gtccaccggc gtcagggtga tcgtggcctt
    13441 gtagttcgcg atcggcagcg ggctcttgac gatggaatag ctgtaggtcg tcttggcagc
    13501 gttgtatgtc aggagcgact cgaggatcgt ggccccgtct ccgagggtca ggaggcgacg
    13561 ggcaccgacc ttggtggcgc tgcccttgag ttcgctcttg atgacggcgg ggtgccacac
    13621 atcgagcgcg ttgaagttgc cgaccagctt ccagaccgtc gcctgggagt tgtcgaccgt
    13681 gacttctcgc gaaaccgaca aggcgcctgc cgcgaagcaa ctgccggtcg cgagcagcaa
    13741 gctggcgacg gtgactttga gggccagtgt tttcatgggg gaaatgtctc ctgaggcagg
    13801 caatgatgat ttgatggttc gcgatggcgc catgcgcggg ccgcgccctc cattctctgg
    13861 cttgagtctc cgcacggcaa cagggctgcc aacgtgaatt ccctgaccac acgggaaagg
    13921 ccggcgtgca agaccagccc ggcgacccca ttcgggcccg gaaattgcct gatcgccccc
    13981 aaccactaac agcaaacgaa ccagcctgca gcgctgtccg ctttgatcca aaacatccat
    14041 gagcatcgcc ccgtacatca aggaaatagg ccgcggcaag gagggcgccc gatccttgac
    14101 cgccgagcag gcgcacgact tgatgagcca ggtgctcgac ggccgggcga gcgacctgga
    14161 agttggagcg ttcacgatgg ccatgcggat caagggggag tcggcggacg agttgtccgg
    14221 attcctcgca gcagtgcacg aacgctgcat acccgtcagt ccggatgggg cagcagtcat
    14281 cctgccgtcc tacaacggcg cccgcaagct gccgaatctg accgcgttgc tagccttgct
    14341 gctggcgcag gagggtgtgc cggtgctggt gcacgggcct tccagcgacc cgacccgggt
    14401 caccaccggt tcgatatttc acgatctggg gttgcccacc gcacgcgatg cacaggatgt
    14461 gcgcaatgcc tgggcgcgtc gcgaaccagt gttcatgtcg attgaaacgc tgtgcccttc
    14521 cctcgcgcga ctgctcgacg tccgctgggt caccggcttg cgcaatccgg ggcataccgt
    14581 ggccaaactg cttgacccga cggttggatc gcggtcgttg cgcgtggtca atcacacgca
    14641 ccccgagtac ggggcgtcgc tggccacttt cctgcagcgt tcgcacgcca atgccatcct
    14701 gctgcggggt accgaaggag agcccgtggc cgaccccaga cgcctgccca ggctggatgt
    14761 gttcctgcag ggtgaactgc gcgccgacct gtcgattgcc gcccaggaag gcgtgctgac
    14821 ggagttgcct gtgctgccac gcaccttcga cgctgcgacg actgcggtct acatccagtc
    14881 ggtggtcagc ggcgaaaagc ccgcacccgg ccccctttca cgtcaggccg actgcatacg
    14941 cagcgcgctg cagaccttcg tcaagggaga ggcgcaggaa ttgatcgcct gaaggggaac
    15001 cgcccctcgg tcgctgcatc ggcaaaatgt gcagaactca tcccgaggct gctgcaccga
    15061 agggcacgga ggacgccggc gaagtcggcg cggggtcaac gacccaggcg ctgtcgggcc
    15121 gattcgtaca ggcagatggc ggtggctgct ccgacgttga gggactcctc gccacccggc
    15181 tgcgcaatcc tgagcgtcag gctgcactgg ctctgcaaag ccgcagaaac gccctcgcct
    15241 tcgtgaccca gcacccagac gcatggccag ggcaatgcaa cttcgtggat ggcctgcgat
    15301 gcgtgcgaac tggtcgccag catgggcgag gcgagcgcat gcaggtcggc gggctcgact
    15361 ccttcgacca gggacagacc gaagtgcgca cccatgccag cgcgaagtac cttcggcgac
    15421 cagaggccgg cagtgccctt gagagccagc acctgactga aaccgaaggc cgacgcactg
    15481 cgcaggatgg tcccgacgtt tccggcatcc tggagacgat cgagaacgat cgatgccgcg
    15541 tcaggatgca cttcggtcac acggacccag ggaacaacaa agcccatcgg cgtcggcgat
    15601 tcgagcgcac tgacgctgga caacagcacg gccggaagca ccgaaaccac cggtgcctgc
    15661 agtgccaggt ctcgcagcgg cgaagcccag ccttcctcgc tgatcacggc ctgcagaggc
    15721 accccgcctc tttgcaggaa cgcacgacag aggtgatcac cctcgatcca caactcgccc
    15781 agcttgcggt acgccaccgg atcgcggccg agcttgcgca gacgcaccag caaggggttg
    15841 tcacgcgacg cgatcgaccg gacctgtggt gtcttcacgc cgacccgccc agtaccgcgc
    15901 gcacgggcgc gaaggagcgt cggtgcgcct cgcatgcacc atgctcgcgc aacgctgcca
    15961 ggtgggcagc ggtcggatag cccttgtggc cgtcgaagcc gtattgcgga tggcactcgt
    16021 ggagtgcaat gcaaagccgg tcacggtgca ccttggcgag gatcgatgcc gccgagatgg
    16081 cctgaacctt cgcgtcgccc tgaacaatcg cctccgcggg catcggcaag accggtagcc
    16141 ggtttccgtc aacgagcacc ctctgcggac gcaggcgcag gccgtcgacg gctctgcgca
    16201 tcgcgagcaa cgtcgcctgc aggatgttca ggcgatcgat ctcctcgacg ctggcctcgg
    16261 cgatgcagaa gcacaacgcc ctggcacgga tctcgtcgaa caggcgatct ctggtccttg
    16321 cactgagctt cttcgaatca ttcagacccc ggatcggctg cagttcatcc aggatcactg
    16381 cagcagcaac caccgggccc gcaagcggcc cgcgccctgc ctcgtccact ccggccatca
    16441 ggcccggtga gtcccagccg aggccgagtt gctcagcgag caaggatttt ctcgatcgca
    16501 tcggtggcgg cctgcgcagt gttgcggcgc aaggtgtggt gcaaggcatc gaagcgagcg
    16561 gccagcgcct ggcagcctgc ggggtcatcc agccatgcga atgcggcatc ggcaagattc
    16621 acgggcgtgg cggcaccctg caggaattcg ggcacggcga aatcctgcag caggatgttc
    16681 ggcagtccga cccagggctg gtagttcatg cgtctcatga tctgcgcact gagccagtgc
    16741 atcttgtaag caatgaccat cggtcgcttg aacagggccg cttcaagcgt ggcggttcca
    16801 ctggctatca gcgtcacgtc gcaggcggcc agggcctcgt gcgagcagcc atcgagcacc
    16861 cggataccga caccgcccgc atgctcggcc agcaaagttt cgatcatcgg gcgcaggccc
    16921 ggcgcggccg gcaggacgaa gcgcagttcg ggacggcgcc gcttcatttc ccgggccgcc
    16981 tcgagcagtg tcggtgcgat gtactggatc tcggagcgac ggcttcccgg cagcaatgcg
    17041 acgacccttt cgccggcgtc caacccgagc gcgcgtcggc tggcggcacg cggtacctcg
    17101 agaggaatcg tatcggccag cgggtgcccg acataggttg ccgcgatccc gtgccgggcg
    17161 tagatctcgg gctcgaacgg aaagatgcac aacacatgat cgacggcgcg cttgatcttc
    17221 tcgatgcgct caccgcgcca cgcccagatg gacgggctga tgaagtggac cgtgcgcacc
    17281 tggtgcgact tcaggcgggc ttcgaggtcg aggttgaagt cgggcgcatc gacgccgatg
    17341 aacacatcgg gcggactctc cagtagccgc tgggtcaggc gatggcggat gcgcagcagt
    17401 tcggggtagt gacgcagaac ctcggcatag ccgcgcaccg ccagtttgtc gctcggccac
    17461 caggcctcga agccctggtc gaccatcttc gggccaccga tgcccatgct cgccaggccg
    17521 ggccagcgtc gggtcagccc cccaagcagc agaccggcca gcagatctcc cgatgcttca
    17581 ccagccacca tggcgagacg cggagcgcgt tcatccatcg cggcgcaaca cagatcgatg
    17641 agcaggtcgg ctcagcgcac gataccgcgc tgtgacctgg caaggaactc gctcatcacg
    17701 cccacgtcga ccgcagcttc gggtgtgctg cccgccagat ccgcgatggc agcacgggcc
    17761 tgctccaacg tgtttccctg gcgataaagc agacggtgca tctgcttgac ggcagcaagg
    17821 cgctcgggcc cgaagccacg acggcgcagg ccctcggcat tgaaaccccg caccgccagc
    17881 ggattgccat ccaccatcat gaaaggtggc acgtcctggg agacggcgct ggcgaagccg
    17941 accatcgcat gcgggccgac cttcacgaac tggtgtatgc cggtcaggcc accgacgatc
    18001 acccagtcgc ccagatgaac gtggcccgcg agtgtggcgt tgttcgccag gatggcctgg
    18061 ttgcccacct ggcaatcgtg cgcgacgtgc acgtacgcca tgatccagtt gtcgtcacct
    18121 acggacgtga tgcccgcgtc ctgtgaggta ccgatgttga aggtacagaa ctcgcggatc
    18181 gtgttgcggt tcccgatgcg cagttcggtc ggctcatccc ggtacttctt gtcttgcggt
    18241 acggcaccca gcgaacagaa ctggaagatg cggttgtcgc aaccgatcgt cgtgcgtcct
    18301 tcgatcacgc agtgaggacc gatcgtcgtt cccgcgccga tgcgcacccg ttcacgcacg
    18361 atgctgaagg gcccgacgct gaccgaagcg tggagttcgg cgcccggttc gatgatcgcc
    18421 gtgggatgga tcagggacat gggacaggcc cgctcgacgc cagcgtcttc gcgttcagat
    18481 ggccttgcgc accacggtgc acatcaggtc ggcctctaca gccagttcac catcgacggt
    18541 gccacgcgcc ttgaacttga acatgccgga cttctgccga tccagcttga cctcaagcgt
    18601 caattgatcg ccaggctcga ccggtcgctt gaaccgcgcc ccgtcgatgc ccgcgaaata
    18661 gaacagcgcg ttttcctcca tcgagatatc caatgtctcg aatcccagga tcgctgccgc
    18721 ctgggccagc gcttcgatca tcagcacgcc gggcattaca gggcgctggg ggaaatgacc
    18781 actgaagtac ggttcgttga tgctgacgtt cttgagcgcc cggatgaaat cgttcttctt
    18841 cagttcaagc acgcgatcga ccagaaggat cggatagcga tgtggaatct tcttcaggat
    18901 ttcgtgaatg tccatcgtca tgacggtttg atctcgttgt tcttttccag tgcacgaatg
    18961 cggtcacgca gttggtgcaa ttgacgcagg gtcgccgcgt ttttctccca cgaagcattg
    19021 tcatcgatgg gaaacaggcc ggtgtacagg cccggcttca gcagtgagcg cgtggccgaa
    19081 gagcgaggac cgatgtggac atggtcggca atcgtcacgt gcccggccac cccggcacct
    19141 ccggcgagcg tgcagtgcgc gccgatgtgt gtgctcccgg ataccgcagc cgtggccgcc
    19201 atcgcggtgt gcgcaccaac gtgcacgttg tgggcaatct gcaccagatt gtccagcttg
    19261 acaccgtcct cgatgacggt gtcctcgagc gccccccggt cgatgcaggt gtttgcaccg
    19321 atctcgacgt cgtcaccgat gcgcacgcca ccgagttgct cgatcttttc ccagcggccc
    19381 gcatttggcg caaagccgaa tccgtccgcg ccgatcacgg caccgctgtg aacgacacct
    19441 cgagcgccga tatgacatcg cgcgcccagc accacctggg gcgagagccg ggtcagactg
    19501 ccgaccgacg caccagcgcc cacgaagcac tgcgcaccga tcactgcacc gtcgccgatc
    19561 acggcgttct gctcgatgac ggcgagcgcc ccgatcgaaa cgtcgcggcc gagtcgcgcc
    19621 agcggatcca ccacagcgct cggatgtaag cggcaagcgc cggaatcccg caggcgcgcc
    19681 gcccacagtt gtgtgagctt cgcaaagcag aggtagggat cgcgtgcgat cagggcatca
    19741 ccgcgctgca cggcagtatc ccggtgcacc gcagacacga tcacgcatcc cgccttcgta
    19801 tcggcaagct gcgagaggta acgagggttg gacaggaagg agatcgcgtc cggcgtcgcg
    19861 ctgtcgagcg gagatatccg ggagatgatc cttgccggat cgccgatcag ttcggcgcca
    19921 agttgcgccg caagatcgcc tagcgcaaac cgtggatccg aatcacttgg caccctgagc
    19981 attcagcgcc ttgacaacct tgtcggtgat gtcgatcttc ggtccggcga acaccgcctc
    20041 ctgaatgatg aggtcgtact tctcagattc gaagatctgc ttgatgacct tgtttgcccg
    20101 atcgaccacg gcagacaact cctcgttctt gcgctgggtc agatcttcct ggaactcgcg
    20161 ccgcttgcgc tggaactcgc ggtcctgctc gaccaggtcg cgctgacggc ggctgcgctc
    20221 ggactccgac agggtcggcg cttccttctc gagcttttcg ctgtttgcct tcagcttgcc
    20281 agcgagatca gccatgtcct tgtcgcgctt ggcgaactcg gcctcgagct tggcttgcgc
    20341 tgtcttcgcc gggatcgcct cgcgcagcac acgatcgctg ttgacgtagc cgatcttgac
    20401 ttcctcggca tgagctgagc atgtgaacgc caacaccacc gacgcggcca acgcgctctt
    20461 aagtaacctg ttcatcaaaa cgcggtcccg agttgaaatt gaagacgctg gattctatct
    20521 gttggctctt tcttgatcgg cgtgccgtaa ctgaccttca acggcccgac aggtgacacc
    20581 cagctcacgc cgaggcccac cgatgcccgc aggctgtcca cggtcatctt gtcgctgacg
    20641 gcccagacgt tgccgacgtc ggtgtatgca aaccagcgca gggtccgatc gttcccggtt
    20701 ccgggcagcg gcaggtagag ctcgctgttg atgttgagcc tgcggttgcc gccgaggtaa
    20761 gcgccgaggt tgtcgacgcc acccagcgat ccctggtcga aaccgcggac agtgccgaga
    20821 ccaccaccga agaagttctt gaagatcggg tatggacgtc cacccagacc cttgccgtaa
    20881 ccgaattcgg cgttcagtcc caaggtgacc cgctggaaag gattgaagta ctcctggaac
    20941 tggagattgg cgcgcatgta ccgctggtct cccagcagac ccaggtcgac gttgacgcgc
    21001 tggaagcggc cgcgggtagg aaccagcgca ctgtcgcgct ggtcgcgcgt ccagcctatc
    21061 gtcagcggca ccgacgaact ggtggagccg aattgctcgc ggtaccggaa gtagctgtcg
    21121 ggcaggttgt tggtgccctg gatctcggtg cgctcgtagc cgatgccgaa gaacaccgtg
    21181 tcgtattcgc tgaatggcac cccgaagcgc accgaacctc caggggtcac cagtttgtaa
    21241 tcttcaccct ggctgttcag cggcgaggac gtacggtaat agagatcgaa tgcgcgcgag
    21301 atgccatcga ccgtgaagta gggatcgatc gtgctcagca cgatggttct ctgcgacttg
    21361 ctggtgttga actcgagacc caggtaattg cccgtcccga acacgttctc ctgcttgacc
    21421 gatgcggtca gggaaatctt gtccgcgctg gagaatccgg cgcccagcaa caggttgcct
    21481 gtgggcttct ccttgatggt caggatcaga tcgacctggt cggaggtgcc gggcacctcg
    21541 ttggtgtcga tgttgacgtc gctgaagtac ccgagccggt cgacacgatc gcgcgacagc
    21601 ttgatcctgt tgccgtcgta ccaggacgac tcgaactggc ggaactcgcg tcggaccacc
    21661 tcatcgcgcg tgcgcgcgtt gccggccacg ctgatgcgac gcacgtagac gcggcgctgc
    21721 gggttggcaa ccagggtcag atcgacgcgg ccattgaccc ggtcgatctc gggacgagcc
    21781 tcgatccgcg cgaaggcata gccgaaggta ccgaatcgct cggtgaacgc cttggtggtt
    21841 tccgcgaccg cttcggcgcg gtacggctcg cccggcttga tggtgacgag cgacttgaag
    21901 tcgtcttcct tggacaagta ctcaccttca agcttgaccg aggtgacggt ataggcctga
    21961 ccctcgttca cgttgatcgt gatggtgatg tcctgcttgt cgggagaaat ggcaacctgc
    22021 gtcgactcga cgttgaactc cagatagccc cggttgaggt aatacgcccg cagggtctcg
    22081 aggtcggcat tcagcttggc gcgcgaatag cggttcgcct tggtgtacca ggtcaaccag
    22141 ccaccatcgt tcagttcgag caagtccttc agggagctct cgctgaacgc ggtattgccg
    22201 aggatccgca gctccttgat cttggccgcc gacccttcgc tgatgttgaa tgtcacgttg
    22261 acgcggttgc gctccatcgg cgtcacggtg gtcacgacct cgacgccgta gaggctgcgg
    22321 ctgaggtact ggcgcttcag ttcctgctcg gcgcgatccg ccagagcctt gtcgaaaggc
    22381 tgcccctcgc cgacaccgaa atccttcagt gccttgacga gcgcgtcttt ctcgaactcc
    22441 ttggccccga cgaaatcgat gctcccgatg acggagcgtt cgtcaacgat caccacgaca
    22501 accgatccgt cgatctggat gcgcacgtcc ttgaacaacc cggttgcaaa caaggcccgc
    22561 agcgctgcca cgcccttctc atcggtgtag gtgtcgccaa tgcgaaaggg caacgacgcg
    22621 aacacggtgc ctgcatcact gcgttgcagg ccctccacgc ggatgtcgac cagcttgaac
    22681 ggttcgaccg cccaggccga gccggcctgc aatgctgtgg ccagagccag gcagatcagc
    22741 gaggggcgaa acgaggtcaa gctggaagga aaacgcaagg tgggtcgcat aggagagatc
    22801 agtgcaggcc catcagtcgg gccacgtcgt tgaagagagc aatggacatc agcgtcagca
    22861 tgatggccac ccccccgcgc tgcaggcgtt caagccacaa gtccggaaca ggacgccccg
    22921 tcatcgcttc aaaaagatgg tacatgaggt gcccaccgtc gagcatgggc agcggcagca
    22981 ggttcagcac gcccaggctg atgctcacga cggccaggaa tccgaggtag taggcgaatc
    23041 cgacgcgcac cgattgccct gcgtagtcgg caatcgtcag cggtccggtg aggttcttca
    23101 gcgaagcctc gccgatcacc atgcgtccga acatcttcag cgtcagccat gaggtgttcc
    23161 atgtacgggt gatggcacgc gacaagccgt caatgaatcc gtagctcacc atcaccatct
    23221 cgggcggccc gccgatgaat gcctctatgc ggccgatgcg acgctcgcca tcgatgagtg
    23281 caacaggctg gaccacgaga tcgatggcct gccccgcccg ctcgactcgc cattgctgtg
    23341 gcgccgggtc agaggccgtg gcacttgcac ggatgcgctc ccgcaagctt gcggcatctg
    23401 ggatgacatc cccgtcaacc gaaagcaccc ggtctcccgc gcgaacgccg cctgtggcgg
    23461 caggaccggc cggcttgacc tcgcccatca cgggctcggt gtagacaccg cccagaccga
    23521 tgcggcggat caattgggca tcgagctcac gcccctcgag tcgctcaagc ggaagcagca
    23581 cgctgcgctc gtgctgcccg cgggcatcgc tgacgcgcag atgcaggctc tcgttctcga
    23641 gcaccgcctg cgtgactctc cagacgaagt cggtcatcga ccgtacctcg ttccaatcct
    23701 cgccgtcggt ggacgttgcc tgcacccaat caccagactg gagtccggca tcggcggcga
    23761 cgctctgggc caacggcgct gccagcaccg ccttgggctc gtccatgccg atccagtgcg
    23821 cagcggcata cagaacgacc gcgagcagca ggttggcaac aggcccggcg gcgacgatcg
    23881 cagagcgctt ccagagcgtc tgcctggtga atgcacggcc cagttccgat gcagcgacag
    23941 gtccctcgcg ctcgtcgagc atgcgcacgt agccgcccag tggaagcacc gacaacacga
    24001 actcggtact gtcgggatgg gactgatgcc gccacaccac acggccgaaa ccgaccgaga
    24061 aacgcagcac cttgactcca caggctaccg ccacgcggta gtggccatac tcgtgaatga
    24121 cgatcagcaa gccgagcgtc agcgcgaacg ccagcacggt ggtgatcatc gcgaaagcct
    24181 ttccacctga cttgacgctg cgcgacgcac ccgcgcgtct agcccgatca ggccctcgac
    24241 ggagctgcac tcgtcgtgtt ccggcagcac cgcctccagg gtgcgggcgt tgacctggtg
    24301 tatgccctcg aatccgatgg cgccggccag aaacgcggca accgccactt cattggcggc
    24361 attcaacacg gccgtgcttc cctccggtcc acgcaacgtc tcccacgcca ggggtaaccc
    24421 gggatagcgc tggaagtcgg ccggctcgaa agtcagtgcg gacagggaag gaaagtcgag
    24481 caagccggca cccgaagtca tccgctccgg aaacgacagt ccgtaggcaa tcggcacgcg
    24541 catgtcaggc gtgcccatct gcgccagcac cgaatggtcg cgacatacaa ccatcgaatg
    24601 cacgatgctc tgcgggtgaa tgacgacctt gatctgctcc ggcgtcaggt cgaacagcca
    24661 gcgggcctcg atcacctcga gggccttgtt catcatcgtc gccgagtcga ccgagatctt
    24721 gcggcccatg acccagttgg ggtgcgcgca ggcctgatcc ggtgtcacgc tggacagcgt
    24781 gttcaggtca cgcgtgcgga aggggccgcc tgaggctgtc aggatgatgt ggtcgatgcg
    24841 tgcaccccag gcactgcggt cttcgggcag gcactggaag atggccgaat gctcgctgtc
    24901 gattggcagc aggtgtgcgc cgccttcccg cacagccgac atgaacaggg ccccgccaac
    24961 cacgagcgct tccttgttgg ccagcatcag gcgcttgcca gcgcgcgcag cagccagaca
    25021 aggaggaagt ccggctgcac cgacgatggc ggccatcacc gcatcgacct cgggatgcgc
    25081 agccatctcg cacaacgcct gcgggccgac cagcacggcg gtttcgctgc cggcatttcg
    25141 cagctgcgcc tgcaaagtct gcgcaggacc agactcggga agcacagcga acttcggacg
    25201 ccatgcaatg cactgcgcca gcagttcgtc gacgcgatga tgagcggtca acgcgaagac
    25261 ctcgaagcgc tcgggatgtc gggacagcac gtcgagtgtg ctggtaccga tggatccggt
    25321 cgacccgagg atgcagatgc gcaagcgact gccggcgttc gcgttgctca tgtcagacgg
    25381 agctcagtgc cagagccagc ggaaacaccg gcagaagcgc atcgacgcgg tcaagcacgc
    25441 cgccatgacc gggcagcaag ccgctgctgt ccttggctcc ggcctggcgc ttcacaagcg
    25501 actcgaccag atcaccgacc acgcccatcg ccgtcaggaa gatacagacc aagaccagtc
    25561 ccaccacacc gtgctgagca tggagctggg tgaacagact cggcgagccc agcacccatg
    25621 tgcgatcggc ctgcatccaa agcaccgcca gcaacagtac gccgatcatg ccgctccaga
    25681 cgccctccca gctcttgccg gggctgatgg ccaacgccag cttgcgtttg ccgaatgccc
    25741 gacccccgaa ataggcagcg atgtcggcca cccacacgag gctgaagatc gacagtatga
    25801 aattgatgcc ctgggtgcgt gcacttgcca gggccaacca agccagccac aagccgatca
    25861 gtcccagcgg ccagcgcaga cccgctggca gaaggcccca tcctgccggc ccggccttca
    25921 aggcccagga acctccaagt acccaaacac ccatcgccgc ccaccaggtt gacggcggtg
    25981 cgctgtgttc ccagcccgca ccgagggcga gtgcgcaggc tccaccgagc agcaaggcgg
    26041 aaccgatgga tgatgacgac cgacctccat tgagacgcac ccattcccac cctgcagcgg
    26101 cgatgaagac cagcgtcagc gcagcaaagg gcaacgggac cggggcaaac aatgcaggta
    26161 gcacgatggc cagcagaacc actgccgtga tgactcgttg cttgagcatc gtcagcgcac
    26221 cagcgggagt tcggccgacg tcgcttcctc gatgccgccg aaccgacgat cgcggcgagc
    26281 gtagtcagcc agtgcagcgt cgagttcggc ttccccgaac tcaggccaga ggcagtcaga
    26341 aaagcacagc tcggagtacg ccgcctgcca cagcaggaag ttgctgatgc gcacctctcc
    26401 gccagtgcgg atgaacagat ccggatcggg tgcgtggctc aatgccatgt agcggctgag
    26461 ggtggcttcg tccagatcat cggcgcgcac tccatcgcgc atcgcgtgct ggcaagcctg
    26521 gaccacatcc catcttccac cgtaattgaa tgcaacagac agcgtgatgc gggtgttgtt
    26581 tcgggtgctg ctctcggcat gatcccaggc ctcgcgcaca tgctcggcga tgtctgttcg
    26641 atcgccgacg atgcgcaccc gcacgccgtt cttgtgcatc acgcccaggt acttggacac
    26701 cgccagcagc acgagcgaca tcaggccgga cacctcgtca ttggggcgct tccagttctc
    26761 cgacgagaag gcgaacacgg tcaggtactc gacgcctcga tcggcgcagg ccagcacagt
    26821 gttcaccagg gtgtcgacgc cttgcttgtg cccaaggaaa cgaggtttga agcggctttt
    26881 cgcccaacgg ccgttgccgt ccatcacgat ggcgatgtga cggggcacct tggaggcgtt
    26941 cggcagagcg ctcactgaac ctggaacctc agcatgcgat gaccgtagtg acgacaacaa
    27001 gcgtcatacc gccatcactt cagcttcttt ggcagccacc agtcgatcga tctcggcaat
    27061 cgtgcgatcg gtgagcttct gcatctcatc gagcgaacgg cgctcatcgt cctcggaaat
    27121 ttccttgtcc ttgagcaggc gcttggcgtg gtcgttggcg tcgcgccgca ggttgcgcac
    27181 ggcaatcctg gcgtcttctc cggcattgcg gacgatcttg gtcaggtcct tccgacgctc
    27241 ctcgctcaag gccggcatcg gcacacgaat caggtcgccc tggctcgacg gattgagccc
    27301 cagatcgctt tcgcggatcg ccttctcgat cttggcgccc atgcccttct cccagggctg
    27361 aacgctgatc gtgcgggcgt cgagcaggct gacattggcc acctgactga tcggcaccgg
    27421 cgatccgtaa tagtcgacat gcacctggtc gaggatgccc gggtgggcgc gaccagtacg
    27481 gatcttctgc aattcggtct tgaacgcctc gaccgagcga cccatcttct gctcggcgtt
    27541 cttcctgatt tcggcaatgc tcatcgcctg ttcctgctca caacggtttc acacatggac
    27601 gagcgtgccc tcgtcttctc cctgaaccac ccgcttcaac gcgcccggct tgaaaatgct
    27661 gaacaccttg atcggcaatt tctgatcacg gcacagcgca aacgcggtgg cgtccagcac
    27721 ctgcagattg cggttgattg cctcgtcgaa gctgatctgc gagtagcgcg tcgcgttcgg
    27781 atccttcttt ggatctgcgg tgtaaacgcc atcgaccttg gtcgctttca gcacgatctg
    27841 cgcgccgatc tcggcgccgc gcaaggccgc tgcagtgtcg gtggtgaaaa atggattgcc
    27901 ggttcccgcg gcgaagacca cgaccttgcc ctcctccagg tactggagag ccttcggacg
    27961 cacgtagggt tcaacgacct gatcgatcgc gatggccgac atcacgcgcg ccgtcatgcc
    28021 ctcctggcgc atcgtgtcgg caagggccag cgcgttcatc acggtggcca gcatgcccat
    28081 gtagtcggcc gtggcgcgat ccatgccgac cgatccgcct gccacgccgc ggaagatgtt
    28141 gccgccaccg atcacgacag cgacctcgca acccatcaag gtcacttcct ggatctcccg
    28201 caccatgcgg acgatggtgg cccggttgat accgtaggca tcgtccccca tgagggcttc
    28261 accggacaac ttgagcagga tgcgcttgta agcgggcatg aaatcgtttt cctgtgctgc
    28321 ttgaagttgc gtggagtcta aaccgggccc acccgccgac tcaggcgatt cggagggccc
    28381 gaaagggctt caggcgccct tggcagccgc cacctgcgcg gcaacttcgg cggcgaagtc
    28441 gtcgaccttc ttctcgatgc cctcaccgac gacaaacagg gtgaaaccct tcacgacggt
    28501 gcccttatcc ttgagcatct gctcgacggt ctgcttgtcg ttcttcacga aagcctggtt
    28561 gaacaggctg acttccttca ggaacttggc aaccgagcca tcgatgcgct tggcgacgat
    28621 ctcggccgac tgcggcgtct tgccttctgc cttggccttt tcggcatctt ccgcagcctt
    28681 ctgcgtcgcc accgaacgct cggtttcgat cagcttggca ggaacgtcgc tcgactgcag
    28741 cgcgaccggc ttcatcgccg ccacgtgcat cgccacatcc ttcgcagcga cgtcgtcgcc
    28801 ctcgaactcg accatcacgc cgatgcgcgt gccgtgcagg tagctcgcga gcttggcacc
    28861 ctcgtagcgc ttgaagcggc ggatcgacat gttttcaccg atcgtaccga tcaagccctt
    28921 gcgcacatct tcgagcgtcg ggccgtagcc gtcctgcgag tgtggcaatg ccgaaagagc
    28981 agccacatcg gccgggttgt tctcggcgac cagcctcgcg caggcattcg ccatcgcgag
    29041 gaacatgtcg ttcttggagg tgaaatcggt ttcgcagttg acttcgacca acgcaccggc
    29101 ctggccgacc acggccgaga cgatcacgcc ttcggcggtg acgcgcgaag cggccttgcc
    29161 agccttgctg ccgagcttga cgcgcagcag ttcctcggcg cgctccatgt cgccaccggc
    29221 ttccgtcagc gccttcttgc attccatcat cggcgcgtcg gtcttcgcgc gcagttcccc
    29281 caccatgctt gctgtgattg cggtcatctt ccgttctcca tagggtctgc ggtttgcgca
    29341 aggcgctgaa ccgcatgagg ctcaaacatt aaaaaaaggg gctgtagcag cccctttaga
    29401 tctcacttcg gtgaggcgat ctgcgggctc agtgcgcttc gctgacttcc acgaattcat
    29461 cgccttcagc ggctacagcc tgcaccacct cgttggtgga gttggccttg ccctcgagca
    29521 ctgcgtcggc taccgccttg gcatacagcg caacggcctt cgccgagtcg tcgttgcccg
    29581 ggatcacgta gtcgatgccg atcggcgagt ggttcgagtc gaccacaccg accactggaa
    29641 tcccgagctt gttggcttcg gcaatggcga tcttgtggta gccgacgtcg atcacgaaca
    29701 aggcgtcagg cagggcattc atgtcctgga tgccgccaat gtccttttcg agcttggcca
    29761 gttcgcgctg gaacatcagc gcttccttct tgatgcggat ctcggtaccg gactcgacct
    29821 gggcctgcat gtccttcaga cgcttcaacg aacccttgac ggtcttgaag ttggtcagca
    29881 tgccgcccag ccaacgctgg tcgacgtagg gcatgccagc gcgagcggct tcaagtgcca
    29941 ccacttcgcg ggcctgacgc ttggtgccta cc
//
LOCUS       DAOORV010000026        29790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1792_length_29790_cov_6.953287, whole genome shotgun sequence.
ACCESSION   DAOORV010000026 DAOORV010000000
VERSION     DAOORV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1792_length_29790_cov_6.953287"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1043..2215)
                     /locus_tag="PKV56_05455"
     CDS             complement(1043..2215)
                     /locus_tag="PKV56_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67293.1"
                     /translation="MSVHGAVWAVFRKEAVDAVRDRRTLMMVLVSAVMAGPLLLVAMA
                     SFLATAQSRNEVRDVVIWGSDRAPTLVNYLQRQSYAIRQAPADYEARLSRSALSAPVV
                     VIPANFEADLQQGELPVVEIVSDSANSQAKSGVARIERMLNGFNRERASLGLALRGVS
                     VALLEPVQVDTRDIANAQTRSAQLTSMLPFFVMMAVLYGTLGAALDTTAGERERGSLE
                     PLLMNPTEGFVIVLGKWAAVATVGVVIAALSSWSFLPTQWVLRSDALSALFLYGAREA
                     SLFMLALVPLAAAVAAALMAVAIRCKSFKEAQASSTLLVLVFSLMPLVTLFNAGASAP
                     WHLWVPALAQNTLMARVLRGESLDVQSVLIPTAVCSALTLICLAFVARSLRRAATR"
     gene            complement(2212..3069)
                     /locus_tag="PKV56_05460"
     CDS             complement(2212..3069)
                     /locus_tag="PKV56_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX67294.1"
                     /translation="MIEVNGLSKAFKVRTRLKRRRDDQWWPWRFGRDQADGGGQLMAV
                     SDVHFRAADGQILGLLGPNGAGKTTTLRMLAGLIKPDAGTLQVDGIDVTRRRAEALAR
                     MGVLSDAHGLYPRLTARENIAYYAALHGLTREAAEARIEMLAGLFDMRRLLDRRTDGF
                     SQGERMKTALARALVHDPANIVLDEPTNGLDVIATRSLRETLRYLRSPAGGAKCIVFS
                     SHIMQEVERLCDRVVVVSHGRTVADGDVDTLLAATGQTDFEEAFVRLAFADSSGLDRR
                     ERRTDRMLR"
     gene            complement(3066..4622)
                     /locus_tag="PKV56_05465"
     CDS             complement(3066..4622)
                     /locus_tag="PKV56_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX67295.1"
                     /translation="MIRQTARITLGLCAWVLAWGLSSGNSHAGIGTEIEWKACRFPGI
                     PTDVQCGSLNRALDPAAPNGLRIEVRLVVLPALSRRKLPDPVFVVAGGPGQSAIAVMP
                     QAVGLLSRLNSRRDLVFVDQRGTGQSAPLRCPNPLRDSLGAQSDPDRQVDLYLACRDR
                     LARLEYVQSVDNLRYFTTPLAVQDLDAVRQALQAERINLVGSSYGTRVILEYQRQFPQ
                     RARRSVLDGVAPADMALPFSVATDAQASFEALMALCAPGSACHRSHPSLRQDWDGLLA
                     GLPRPVVVAHPLTGEAEAFEMTRHAVLSMTRGALYSPALASALPQAISDAARGNFAGM
                     VALGGLLPGRKATALAMGLHFTVVCAEDFPRLAQSTDRPGPDFGRAFSSIYERVCRDW
                     PRANLPADYGRAVHSPNPVLILSGGLDPVTPPRHAERSLLALGSVASGGLAVHRVVPN
                     AGHGVLSLGCMRDALFRFVDASDDRQAVASAQQIECGNDFPRVPAFRPVGRRPSSAST
                     GSAAPAESAR"
     gene            4759..6459
                     /locus_tag="PKV56_05470"
     CDS             4759..6459
                     /locus_tag="PKV56_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX67296.1"
                     /translation="MWLISARSTLRGPVTTRIISLFHVGCWSHSNKTSSCLQLPAHDQ
                     FPCTDCVSRFSFNVNLSSLRQAPAATQSEVARAIPAASGSMRRLQIQLSLSNFALVFL
                     TSVLGVVGLGLYDRVSSENLRVADLSTEVQSMRSNLYRQVKEVIDDVLLGDPEAVQQF
                     AQLSAAGDDQFARLTTLLRTAEEQRAASDLRLAYVEVRELGDRMIREARESPPDRSEQ
                     QRVLHTGIEHGSLQRYERALVAMRDIVAHERAERGHQLLVLKRWVGAGLLLSLVCGFA
                     LLALSSELLHRRFARPLSDVLASIRRISQGQLEHRVTDSGVAEIRELALGINTMSSEL
                     SASRLKLVHAEKQAMLGSLVPVVAHNIRNPLASIRATAQIIDAPALPADVREGLHDII
                     SASDRLESWTHSLLSYLNPLQPNCQWVTLQTVVETCLGLLSARTAERELSIRRMGWDA
                     PVRVEADVALVEQALTGLLMNAIEASPVRGQVTLAADTDGGIVRLSIRDQGSGMAVLP
                     SASRRLTMRSTKPQGNGLGIPFCRKVCEGHGGQLNFVGHADGTEVIVTLPQKAVHLAT
                     "
     gene            6485..7912
                     /locus_tag="PKV56_05475"
     CDS             6485..7912
                     /locus_tag="PKV56_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX67297.1"
                     /translation="MAGTLPRLSCSILMVEDEAMFARAVCKRLAAAGYACRSVGTLAE
                     AREALADAPDLILLDLRLPDGEGFELLSQLSTEHVRPTVVVMTAYGELAPAIEAMKLG
                     AADYIKKPIDLNELLLVVQKVLSTAQLRQRVDYSRERESHSAEGMELLGNSALLRATR
                     TRLESLSRLCATPGVAAPTVLLLGETGTGKDVAARLLHRSGPLSGRPFVQIDCASLPR
                     ELIEAELFGHEKGAYTGAQSKRVGLIEAAEDGTVFLDEIGEMGLDLQAKLLNVLERRV
                     VRRVGAVREYPVAARFVAGTNRDLPRMVAQGQFRADLFYRLNVIAVQMPTLRDRRNDI
                     VPLARHFAQITARRYGLQEPNFEETALDALVRYPWPGNVRELKHLIERAVLLSGHTIS
                     ASELALPVPEAAVPQSAAAGEEDPTPMGREMIHMTLEEAERHLIEHALHLTRGNVSEA
                     ARRLGVTRMALRYRIDKLKLSPASG"
     gene            8255..8632
                     /locus_tag="PKV56_05480"
     CDS             8255..8632
                     /locus_tag="PKV56_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67298.1"
                     /translation="MIKSVKQFLKDESGQSMVEYGITLGGVAAVSYLAIVVLGDKTGD
                     LYAWMANHLPGGESGETAQVRANGEDGLVGTNVVTGADGIVAETFANRSDDATGLTGD
                     WTLNGTLGTSSIYASADGGWNAD"
     gene            8855..9493
                     /locus_tag="PKV56_05485"
     CDS             8855..9493
                     /locus_tag="PKV56_05485"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOX67299.1"
                     /translation="MSIIETLTAPVSSLFSNGIGPAYLLGLAVLSLAVAVSDARAHRI
                     PNAYLAAGLLYALLLLGALASTRGPAFALRGLGMGLFGMLLGGLLSAPGLFARQLAPG
                     DVKFMMVIGFFLGPIGAVFALLNAALVGGVWALVLAWRHGGLRLLGHNLKFMARSAYL
                     SGFKDLGWDLRQAGAVRMPYGVALAVGAWSVIAWQVTRSQSLRAALAPLLGA"
     gene            9503..14365
                     /locus_tag="PKV56_05490"
     CDS             9503..14365
                     /locus_tag="PKV56_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX67300.1"
                     /translation="MASAASSVSKARPNAVQSAVARDRGLRESQRGQKLLEAGEMSPA
                     IARFQQALQHDPACVPAISGLGEAAFRDGRYDLAEGLFRKALEHAPNDPMLWGWLGTT
                     LNSLRRHDEAQQHLLHARSLGLAIGDIDRGLGIVALAQGRLDEAEAHWRQALATDYLS
                     ASVRSMLGGLLLGRGRWLEGWQLRAENPTRSAEKIPRPEWQGIDLSRRDVLLLRNQGL
                     GDELFYLRWLPALQARGAQVVLRCSARLQPLLAGLPGVAELVPEIEGAEVPLRARWQG
                     HPLCALVDDLPLALGDEAASATAPSLRLTPDAAHLARLRARLAALGPAPYIGLTWRAG
                     TAAQADNSHERLIRVAGGGAVRTRPLDKSLPPQALGEALRGVAGTLLALQRQPMPGEL
                     EAVAAAAGQPVHDLCDLGHDLAELLALMALLDELVGVSNTNVHLRAAVGRPARVLVPH
                     PSPDWRWMHEGDRSPWFPGSTVYRQTPTGDWSPALERLHADLLSPSPLEIPSAGPSTC
                     ASTGLRSTPPRSPAACPDHDLVRQGLDLIATGEHAAAKSHFIGMLQSDPSNAAAVHGL
                     GRVAHAESRYDLALQLLTQAVAAAPARFDWQGDLGLAHLALDDHARALPLLQAAAASE
                     AASADHRRALGFLALQHGDWSGAAAHWQAGLARTPGHRPCSGLLGRWHLARQQWAEGW
                     ALVDRLPTRCPAAVSAVLQPWPDAAALRGARVRVVVTEGLGDELFALRFAPALASLGA
                     SIDWLAEPKLLPLLRRLPGLDSVIAFEGTEAAVPHGAAEPDYTVLSGHLPRLLWQHSP
                     SPTPPSARVAPLADRLRALEARLIASGPRPWLGLTWQAGQAQPGPAAERTFRGPDGSV
                     IKQRALSKQVPASQFARCTDGLPGTVFLLQRGVDAAAREAFAQACGRPVHDLTDLSED
                     LEDLLAVMALLDAYAGVCNTNVHLRALAGRTSEVLVPHPVPEWRWLLEGDESPWFPGC
                     GVHRQRADGDWGPAVARLQRALQSTLQSASNDSTKRLSTGQTLPHAGTTLAADGKAAA
                     GPAVSDVDGVVFWLTHGSISLRDGQPWSELASARLRALLPAAGLAPAGLRSRFLSETG
                     CPESLSGNEPPTPGDTLVIGKSFSHDTTKLLLQSKKQGTRIVVDVCDDHFDHREHGPH
                     LHFLVTAADAVTAATPAMAARIAQASGRDATVIGDPYEGPAGVAHFQPPAAGDARPLR
                     LCWFGHPVNHDTLAAALPAVVALAATRPVHLVAVTHRAQLGPVLQRHTKAAKRGATGA
                     AGVLTTHIEPWTPEAVWQALADCDICLLPTLDGPGKSVRSANRLIEALRAGRCVVAGP
                     HPAHEPFAECAYVGDLAQGLQAALADPAATLARIRSGQERVADTCSPDVVSQAWAQVL
                     QRPAPPAATEALSPAAPSRATTLAVAVPAIPAAAVRYPDPDSPPAPVAATATAASAVR
                     LNLGCGDKILPGYVNVDVVESRAGKRPDVLCDLHRLEPFADDSADEILAVHVVEHFWR
                     WEVLDVLKEWVRVLKPGGLLVLECPNLISACETFLRDPEVAASGGKEGQRSMWVFYGD
                     PQWQDPYMVHRWGYTPKSLAQLMGEAGLVDARQEPAQFKLREPRDMRVVARKPVRLSN
                     AGGIRIVHFK"
     gene            14378..14974
                     /locus_tag="PKV56_05495"
     CDS             14378..14974
                     /locus_tag="PKV56_05495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX67301.1"
                     /translation="MGDSNQLYYLRRHVPSAHGPVLEIGSKDYGNTADFRAIYRDVPY
                     VGLDLASGRNVDVVHDLTKGIGPLAPDHFDLAICCSVLEHVDRPWDFARHVSRVVRPG
                     GLLYMSVPWVWRFHAYPDDYFRFSWRGIEVLFPDFEWRERYYSTNVDDEFISIDEFRD
                     ADNRMSLLQEVEGGLRKHLPYLMLNMLGTRRHDTPAID"
     gene            14995..16167
                     /locus_tag="PKV56_05500"
     CDS             14995..16167
                     /locus_tag="PKV56_05500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX67302.1"
                     /translation="MHSRNIAYLPAVDQVRGFAALWILFYHSYQLLGSRLRTGEPFRK
                     ETMWERDAGPLLAPLVEGHTAVALFMVLSGFIFTWGAIGQRIDYLRFIGNRFLRIYPL
                     YLVLLMVALAARPRAFELDVVLAMLLPFADFVRPGTGPLTGMSWAIGVEFQFYLLFPL
                     LFALARDNPARLIAGWIACAVLLRLLAVGLGGSAYHVSYWHLAGRIDQFLLGMAAAFW
                     LAQARPEPRRCVLLLCMAVPMSVALLHAFHRGGGLPVSAPWKTIWPAIEGAMWALCIA
                     GWIGLRGAGQRWPARVLQWVGTRSFSIYLLHYPLVQLLAAHPGLWWAPTGEWRTDALL
                     TSACVVLPLSLLLSSLTWSAIEKPFLSLRRSYLTPLDGPPSPLTAHPNTASVARAP"
     gene            16181..16876
                     /locus_tag="PKV56_05505"
     CDS             16181..16876
                     /locus_tag="PKV56_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67303.1"
                     /translation="MIPVYIGFDPRETVAFSVLSHSIHRRASEPVAIAPLMLSQLHRQ
                     LWRERHPLQSTDFSFSRFLVPSLSGYAGWSIFMDCDMLVLEDIARLWSLRDERYAVMC
                     VQHDHRPVEDRKFLDAVQTKYAKKNWSSVMLFNNARCTALTPEYVNTANGLELHQFKW
                     LAEDGLIGALPARWNHLVGYDAPDPAPALVHYTQGGPYFDEYADCEHAGRWRAERESM
                     LRCEQRLPARAAA"
     gene            16893..18068
                     /locus_tag="PKV56_05510"
     CDS             16893..18068
                     /locus_tag="PKV56_05510"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOX67304.1"
                     /translation="MPPATPTRSARPQRGQALIEFAIILTLLVLLLAGGLELGTAAIN
                     SNQARQAAEQGGQQWSEVVGASGLYVAAASSPYAIGNGAVVPAPATVGLGDHTPANFA
                     RPACDDLACTAPSNGLTGPSAALNPPIDPATGALTGDLYLFNPLPIDVTSCVVNDAVQ
                     YTDCVNRIFDGCGPPTCPAAVAGLPRLNAALRGLYTLRCLNAADAQVVCRSVASGGTA
                     VTWLLRLPGRHDVAGDDTVRLALLDDTSNVTAEVPIFEIECAAAGGAFIDAGGNPCDT
                     QAAPGEVCWTAAGTALACRVRVKVRYRHHFYALLASAFFDGDPLPVLTAAALDLGEVN
                     AAGVPVGGYGAEVTAQELASAAPKYFKVPWRTFQGCGSTRTVRDGTSGLRGSTANCN"
     gene            18095..18991
                     /gene="cpaB"
                     /locus_tag="PKV56_05515"
     CDS             18095..18991
                     /gene="cpaB"
                     /locus_tag="PKV56_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOX67305.1"
                     /translation="MVTTVITARQRRARNQRIVLLTAAVLLVGGGLWYLLRGEERAPA
                     AEKQPLGTIAVPVAARPLPKGRELEGPGLIRPMYLRPEQVPPAALLKTNQIVSRVTRE
                     SLKAGDYFSEENLTAAGAPPGFSGLVRPGSRIVVIETTRIAGVIGYMREGDLVDVMAL
                     SQSAGAGARPGGGPTIEAGGTQPGSGGQAAGSGAARGGGGSLPGLTATLIAQGARVVI
                     VPKPGKNQFAALEMAPEDAHLLTMVMSTNQVLHFVYRPFNDDSRLDADSPALAETRPP
                     RDSRTIEMIEGVNRSVLRTTLN"
     gene            complement(19123..19491)
                     /locus_tag="PKV56_05520"
     CDS             complement(19123..19491)
                     /locus_tag="PKV56_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOX67306.1"
                     /translation="MMKRVSMFCDAADGASTLAADVLVANRYFSRLRGLIGHPPLQPG
                     RGLWIVPTQQVHTHFMGAPIDVIFLDRDMKVLRVVPALAPWKLSPWVKGARSALELAA
                     GTAATVAVGDRLVARPCGHE"
     gene            complement(19505..20365)
                     /locus_tag="PKV56_05525"
     CDS             complement(19505..20365)
                     /locus_tag="PKV56_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOX67307.1"
                     /translation="MNSTLVVLSIAALIGLGVLYVVRASQLAVLRERLSFGLDLMGER
                     AGDDLTAGDYGLVGTDRLGYHFALFVGSWRGRVLVAALAGAAGFGIAYSQGRKLDASI
                     TLSVASSLAMTLGSMAYLYSRRRRREQRIRKALPDMLELVAAIMEGGIAFETALQHVV
                     RDSDLRHPLYFELSVMLEAMRRGRRRHEALRLFGERCNIVQVAEIVSSLTQADQTGSS
                     IATVLRHHARTLFREYEAEVQRQAERLPIRMMFPMLVTILPAMFIVTGLPSLLRIYRV
                     MESIIASRPG"
     gene            complement(20367..21311)
                     /locus_tag="PKV56_05530"
     CDS             complement(20367..21311)
                     /locus_tag="PKV56_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOX67308.1"
                     /translation="MQLLIGLLIVAAAAIAVLAYRESVRSERLRERLSIEAPDESPDG
                     SLWRDAADLLQIEQLQALLERSPLTRQFAERLVRSNLPLGLGRALFVVVVSTSVAVAL
                     ALVFQRTLWLPIAALVAVPLLMWLALDAYADQRVQRIDRQLPAFVAQMLAALRTGGTP
                     LSSLQTAARISPQPLGGTLQALLDALQLGVTPTQAWRDWAIRCGGAHCELLATGVRLK
                     WEAGGQMTSMLEHILESMQSRERMILRVRTLTAQARLAGYVLTALPLLFMLFTYYRNP
                     RIFSFMLNDPLGVNTLWAGGALLVLGFFWMRRLARLDV"
     gene            complement(21348..22799)
                     /locus_tag="PKV56_05535"
     CDS             complement(21348..22799)
                     /locus_tag="PKV56_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOX67309.1"
                     /translation="MPDDADRGIPEHAPTLRDTLRRNTASQTDFLGSRMRALASGQKD
                     ARLINEQQQASGSLATAALSTAVNVNSATYSQVRHRLHRGLLDVLNPQAVANATMPQI
                     QAAIEQYLDDALARDTLPLSRMERAQLSDDLLHEVVGLGPLSPLMSDPTVSDILVNGP
                     HQVYVERHGVLELTRIRFHDNEHLMLTVERILSRVGRRVDESSPMGDARLADGSRVNV
                     IIPPIAVNGPTMSIRRFMREILGMDELVGNGALSAAAAEFLRLAVQARMNIIVSGGTG
                     SGKTTMLNCLSQHIPEGERLITIEDTAELRLDHQHIITLEARPANAEGRGSVSIRDLV
                     RNALRMRPDRVIVGEVRGAEALDMLQAMNTGHDGSLATLHSNSPRDTLSRLETMMLMA
                     DVDLPQRVLREQVASAVDFIVHLARFADGSRRVTHITEVVDVDNGTVLTQDVFTVRRD
                     GSRVVMYPCGIVPSSAPEFFERGVPLDPALFSD"
     gene            complement(22807..23997)
                     /locus_tag="PKV56_05540"
     CDS             complement(22807..23997)
                     /locus_tag="PKV56_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX67310.1"
                     /translation="MRILAISAHEQFRQPVQAACLRYGETLVSMRLSDSIGQGLELAK
                     QFEAEVVFFDLTRNVEAGMIAIEQLAAVPYARLVVASVDKSSTELMSRAIRAGAREFI
                     AQPPQEDDIHAVLRKAQRQMEPQPLQDGARGRIIVVFSSKGGVGKTTISCNLAVVLAQ
                     KLGENKVAVIDANTQAPNVAPMLDLRPEHFLRDAIEQYRRLDIEMLQQFMTLHSSGAQ
                     VLAHSSDNPLGLDFEEDQLSKILLVAKSAYEQVIVDTFPVLSSLNLALMDLADEIVLV
                     TEPVVPALRSARYNLQVLKQAGYGPERILVVLNRYTRFRGNVPIDLVAETLEWPIAGV
                     LPYDVHATIAANSGRAVVEMFPNVPLSDELELLADRLIGEQERLPVFRPSLIERLSRF
                     VRGY"
     gene            complement(24055..24729)
                     /locus_tag="PKV56_05545"
     CDS             complement(24055..24729)
                     /locus_tag="PKV56_05545"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOX67311.1"
                     /translation="MNRGSTLRRVAAGVALGLALTGCHHLRPPHGSMATGLAPGFAAE
                     AGGAAHPADTDAIDDPLTRANYEAADRLLATDATQPEVLGTVVVATVVSQDDLDRSTA
                     LGRLVSEQVAGRLAQRGLAVREVKLRGSLFVARATGALMLSRELSEIARSHDAEAAVV
                     GTSARGAQGTWVSLKLVRLADARVLAGWDYLLGVDDSLGQSPANTAPPLTLYDAVRAY
                     GASRAR"
     gene            complement(24726..25352)
                     /locus_tag="PKV56_05550"
     CDS             complement(24726..25352)
                     /locus_tag="PKV56_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67312.1"
                     /translation="MNSLFSNPAGTGQHIGARVAGVVLCSAFLLLAGCARVPLDPVPP
                     AVVSLPQPALHALTHWELGVRGLAADVAAALKAGPGGAPLRIDTPEPSVFTDTVRDLL
                     ATQLHEQGVAVWRPGDPRATELRGPVLSLHTRLIPFGAGHIGAQSLSTAPPRHELVLT
                     ATLDAGGPPGLRRSLLFYAADADLALYLPARDRSVSLKVVGSLAGRKP"
     gene            complement(25349..26014)
                     /locus_tag="PKV56_05555"
     CDS             complement(25349..26014)
                     /locus_tag="PKV56_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX67313.1"
                     /translation="MALGWLRGRRAGLYGIALVLLGLAAAGLWYLKTTQDREIADALA
                     AIEAAAALAPDKASGACPPLMERARQMAQSIAADEALALEASRAYRALGQCSLLLNKL
                     DAAAADFRSAIAHRAEFGPLHGDLAQVLSRLGQHNPAQRSAGLAIQLDPQVWVAYRLH
                     ARVLEAGGKVGEAERSYEEALRLAPPEQIKKLQDEINRFKQAHESARTTRLIDPPQPR
                     SDP"
     gene            complement(26014..28032)
                     /locus_tag="PKV56_05560"
     CDS             complement(26014..28032)
                     /locus_tag="PKV56_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOX67314.1"
                     /translation="MRSARLFRPTRRLAKWLCSVGAILSFGAARAETFEVLRSHSRLV
                     EVPFDVASIAVGESDIARATAVTPRRVLINAAKFGATSLMFFGRKGELESHLVHVVHN
                     TDALRAQLMMIDPRIKVASDPNRNAIVISGLVRTEQLKRRALETALSFVGNTRYTVLD
                     MPLVLSRDDDASVEDISTDVGPANVLAQTPGSTRQRTPAGGRPMQARLPLNTESVISA
                     VRTTDANAPQIIDLLASDESFVAPHGQLRQMLAAIDASLRVTEVNTVLHVTGKVDTPI
                     QLTRALTLIDSYVGVGTASGDFRPVSDRGGVLVANNSNVGSGGQGGEGGNRRSQSSAF
                     GGGSSSGFSSSNRGSAGSAGIAISESPAKGNIALNLSRSSAVSIAGGRALSLIEVRDQ
                     PRVEVQVRIVAVDRDATDKLGFNWRLDGSHVSIGNLTGGVVDTLPSASGSSTNIGGGG
                     SGGSGAGNNNAIDVGTANIVGFFRSNRVSISAFLQAIEEKGAGTALSEPLLTAVSGEI
                     VRFLVGGEIPIPVANTSISTNSAVGGSTTTNQNTLEFREFGIQLFLRPTVLEDGRISL
                     VLDQSITQPDYSRSFLSGGNQVPSFSKRAVQTITESADGETWAVAGLLTQEDSDRLEK
                     VPLIGNVPILGKLFQSSNKRRARSELIITITARKVPLPAPVTAAREVR"
     gene            complement(28306..28752)
                     /locus_tag="PKV56_05565"
     CDS             complement(28306..28752)
                     /locus_tag="PKV56_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67315.1"
                     /translation="MKYRFLLSCAALCLWGGPVGAAGDLTRQEPVIVELKLGQVGGEH
                     RFTPDSLVFETGKLYVLRLENPSANAYYFGSQGLADSVYSRKVVALGPDGKVLAEVYG
                     PVRRFELKPGSLLEWWFLPVRTGRFDDVMSTRAHTESGMRATIEIK"
     gene            28934..29290
                     /locus_tag="PKV56_05570"
     CDS             28934..29290
                     /locus_tag="PKV56_05570"
                     /inference="COORDINATES: protein motif:HMM:NF023420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HOX67316.1"
                     /translation="MNTKATRKTTQPASKAALPVKTAPPALKAAKRTVRTLKTQPLPV
                     VAVAAPPGSKQSQLLAMLRSPTGATIEQMTGATGWQAHSVRGVISGVLRKRLNLDVVC
                     TPDPAGGARIYRVAAA"
     gene            29320..29685
                     /locus_tag="PKV56_05575"
     CDS             29320..29685
                     /locus_tag="PKV56_05575"
                     /inference="COORDINATES: protein motif:HMM:NF022598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HOX67317.1"
                     /translation="MDRPALALRWESTFGVPAPKSCQATLLRQALAWQVQMSALKTRV
                     GVREADRGTRALRTSSSSSAIAPGTRLLREWQGRTHHVTAVVGGFEYDGRVWRSLSAI
                     ARAITGSSWSGPLFFGLRS"
     gene            29691..>29790
                     /locus_tag="PKV56_05580"
     CDS             29691..>29790
                     /locus_tag="PKV56_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOX67318.1"
                     /translation="MPCAVYTRKSSEEGLEQGFNSLDAQREACEAFI"
BASE COUNT         4949 a        10120 c         9953 g         4768 t
ORIGIN      
        1 tgaaagtcgc ggtgatggtg gcgcgcacag ccgacagaga aagctgcacc gcccgcagac
       61 ccgcgccgcg cgggcttctc caaagaaaaa gccaccgcgc tgcggtggct ttgaaatgtt
      121 ggcggtgggc acagtcgctg gcgaacccgt ctcagacgta gcctgaattc cctgcaacag
      181 ggaaataaac agggacttcc gtgccgcggg cgaacccagc cgtgccacgt cggcgccctc
      241 ttcccaggca aacccgccag ccccctctca cggctcgctt cacgaacagg gaattcgttc
      301 gccggtatca gggaattcaa gacccgcttt cagcgattcg cggcacattc ttgacccgct
      361 tacatcacca acctacgacc gcggaaaatt cgcccaaacc gcttgctagg tggttcatcg
      421 cacgctacga acgctctccg tcctgaaagc aagcgctaca cggcaaggcc tcgcgaggtg
      481 gttctcgatg tgctaaccac tgcacgccct gcccgactcg gcagccccga cttcgcaccg
      541 atccatgggg ctttcctcgc cggctcagcg ggaagcgctg atgttgacgt cgcgccaggc
      601 gggggcggcg aaacttgcga cgcgatggcc tccacatgcg ccacggaggc cgacttccga
      661 ttagtcatgg cgccatcaaa gctgacgttg cgaccagcgc gcgtcgccag gccgatcggc
      721 acttgacaca aggagtccct tacgcgagat gtgatgccgg cagtaccgag cacaggccca
      781 ccgacgtgaa agcattcccg tcgggtccat tgattctcaa tgcagtgccc ccccggaagc
      841 tagaccactg gggccgccag agccaagccc cctgctgggc gagttgagca ttgaactcgt
      901 tgaaaggaag atcggtcggc attgcgtcag tacacgccgg acccgtcctc gccaagtctt
      961 ttagaaattt cggcttcagc tgaacttgga ctgactttgc ataagctctt cagccgcgac
     1021 aggcggtcac tgatccactg aatcaacggg tggcggcacg tcgaagtgag cgcgccacga
     1081 aggcaaggca tatcagggtc agggcgctgc agaccgcggt cggaatcagc accgactgga
     1141 catccagcga ctctccccgc aggacgcggg ccatgagcgt gttctgcgcc agtgcgggaa
     1201 cccagaggtg ccacggcgca ctggccccag cgttaaacaa ggtaaccagc ggcatgagtg
     1261 agaagacaag caccagcaag gtgctgctgg cttgagcctc cttgaagctc ttgcagcgaa
     1321 tagccacagc catcagagcc gcggccactg cagcagccag cggcactagc gccagcatga
     1381 acaaggaggc ctcacgagca ccgtaaagaa acagcgccga cagtgcgtca ctgcgcaaga
     1441 cccactgggt cggcaggaaa ctccatgaac tgagcgccgc tatgactacg ccaaccgtcg
     1501 ccacggccgc ccacttgccg agaacgatca cgaatccctc ggtggggttc atcagcaacg
     1561 gctcaagcga gccgcgctcg cgctcgccag ccgtggtgtc gagcgccgcg ccgagggtgc
     1621 cgtaaagcac cgccatcatg acgaagaacg gcagcatgct ggtaagttgg gccgagcggg
     1681 tctgcgcatt ggcaatgtca cgagtgtcga cctgcacggg ctccagcagg gcgaccgaca
     1741 cgccccgcag cgccaaccct agagacgcgc gctcacggtt gaatccattc agcatgcgtt
     1801 cgatccgggc cacgcccgat ttggcctgcg aattcgcgct gtcgctgacg atctcgacca
     1861 ccggcaactc gccttgctgc aggtcggctt cgaaattcgc cgggatcacg accacaggcg
     1921 cgctcagcgc cgagcggctg agtcgagcct cgtagtcggc cggtgcctgc cggatagcgt
     1981 aagactgccg ctgcaagtag ttgaccaagg tcggcgcgcg atcgctgccc catatgacca
     2041 cgtcgcgaac ctcgttgcgc gattgagcgg tggccaggaa gctcgccatc gcgaccaaca
     2101 gcaacggtcc ggccatcaca gcggacacga gcaccatcat cagcgtgcgt cggtctcgca
     2161 ccgcgtcaac ggcctccttt cggaacaccg cccagaccgc cccgtgcacg ctcatcgcag
     2221 catccgatcg gttcggcgct cacgacgatc gagtccggac gaatcggcaa acgcgagtcg
     2281 aacgaatgcc tcctcgaaat cggtctggcc cgtagccgcc agcagcgtgt cgacgtcgcc
     2341 atcggcgaca gtgcggccgt gtgacaccac caccacgcgg tcgcaaaggc gctcgacctc
     2401 ctgcatgatg tggctcgaga acacgataca tttcgcgcca ccagcggggc tgcgcaggta
     2461 ccgcagggtc tcgcgcagcg atcgcgtggc gatcacgtcc agcccgttgg tcggctcgtc
     2521 gagcacgatg ttcgccgggt catgcaccag ggcgcgcgcc agcgcagtct tcatgcgctc
     2581 gccctgactg aatccgtcgg tgcggcggtc gagcaatcgg cgcatgtcga acagccctgc
     2641 caacatctcg atgcgagcct ctgccgcctc ccgcgtgagc ccgtgcaacg cggcgtagta
     2701 ggcgatgttc tcgcgcgctg tcaggcgcgg atagaggccg tgcgcatccg agagaacccc
     2761 catgcgcgcg agggcctcgg cgcgccggcg ggtcacatcg atgccatcga cctgaagtgt
     2821 tccggcatcc ggtttgatca ggccagccaa cattcgcagg gtggtggtct tgcccgcgcc
     2881 gttgggtccg agcaatccaa ggatctgccc gtcggcggct cggaaatgaa catcgctgac
     2941 cgccatcaac tgcccaccgc cgtcagcttg gtcgcgaccg aaccgccacg gccaccactg
     3001 gtcgtcccgg cgccgcttca agcgggtccg aaccttgaaa gctttggaca gtccgttcac
     3061 ctcgatcatc gggcggactc cgcaggcgcc gccgaccctg tcgacgcaga tgagggcctg
     3121 cggccgaccg ggcggaacgc tggcacccgc gggaagtcgt taccgcactc gatctgctgg
     3181 gcgctcgcca ccgcctgccg atcgtcactt gcgtccacga agcggaacag cgcgtcacgc
     3241 atgcaaccca ggctgagcac gccatggcca gcgttgggaa ccacccgatg cactgccaac
     3301 ccaccactcg cgactgaccc cagcgccagc aggctgcgtt cggcgtgtcg cggtggagtc
     3361 accgggtcga gtcccccgct caggatcagc acagggttgg gactgtgaac tgctcgcccg
     3421 tagtcggccg gcagattggc tcgaggccag tcgcgacaga cgcgttcata gatgctgctg
     3481 aatgcccgtc caaaatccgg gcccggtcgg tctgtggact gcgccagccg ggggaagtcc
     3541 tcggcacaga ccacggtgaa gtgcaggccc atggccagcg ccgtcgcttt tcggccaggt
     3601 aacaggccgc ccagcgccac catgcccgcg aagttgcccc gcgccgcatc tgagatggcc
     3661 tggggcaagg ccgaggcgag agcggggctg tagagcgccc cgcgggtcat ggacagcacc
     3721 gcatggcggg tcatctcgaa ggcctcggcc tcgcccgtca acggatgcgc caccacaacg
     3781 ggccgcggca gtccggccag gagcccatcc caatcctgcc gcagggaggg gtggctgcga
     3841 tggcaggcgg agcctggcgc gcaaagagcc atcaaggcct cgaagctggc ttgcgcatcg
     3901 gtggcaacac tgaagggcag cgccatgtcg gccggagcca cgccatcgag cacgctgcgc
     3961 cgcgccctct gcggaaactg ccgctggtat tccaggatca cacgcgtccc gtaggaactg
     4021 ccgaccaaat tgatgcgctc ggcctgcagg gcctgccgca ccgcatcgag gtcttgcacc
     4081 gccagcggcg tagtgaagta ccgcaggttg tcaacggact ggacgtactc gagtcgggct
     4141 agccgatccc gacacgcgag gtagaggtcg acctgccgat cggggtcgga ctgcgcaccc
     4201 agcgaatcgc gcagcgggtt cgggcagcgc agcggcgccg actgccccgt cccgcgctgg
     4261 tccacgaaca ccagatcgcg tcgactgttg agccgcgaca gcagtccgac ggcctgcggc
     4321 atcacagcga tggcactctg gcccggaccg cccgccacaa cgaacaccgg atcgggcaac
     4381 ttgcgacggg aaagtgccgg cagcaccacc aagcgcacct cgatgcgcaa gccattcggt
     4441 gcggcagggt ctagggcacg attcagcgag ccgcattgca cgtcggtggg gattcccgga
     4501 aatcggcagg ccttccattc gatctcagtg ccgatacccg catgggaatt tccgcttgac
     4561 aggccccacg ccagcaccca cgcacacagc ccgagtgtta ttcgggcagt ctgtcgaatc
     4621 atcggatcgg ttctcggagt tggcgtgcac gcctgcaaca gcaaatcccg cgcccaagat
     4681 gcgcgggaag gctgcctagg ggcacggtcg caccgttgcg gcgcgctgcc ggcacgcggt
     4741 cgcggctgac agcacccgtt gtggctgatt tcagccagaa gcactctgcg cggccccgta
     4801 acaacaagga ttatttccct gttccatgtg gggtgttgga gtcacagtaa caagacgtct
     4861 tcctgtttgc agctacctgc ccacgaccaa tttccctgca ccgactgcgt gtctcgtttc
     4921 agtttcaacg tgaacctttc atccttgcgg caggcaccgg ccgcgaccca atccgaagtt
     4981 gcgcgcgcca ttccggctgc gtcgggttcc atgcgtcgac ttcagatcca gttgtccctg
     5041 agcaatttcg ccctggtgtt cctcaccagc gtgctgggtg tcgtcggtct tggtctatac
     5101 gaccgcgtat ccagcgaaaa cctgcgcgtc gccgacctaa gcacagaggt gcagagcatg
     5161 cgcagcaacc tctaccgcca agtgaaagag gttatcgatg acgtactgct cggcgatcca
     5221 gaggcggtgc agcagttcgc tcagctatcc gccgccggcg acgatcagtt cgcacgcctg
     5281 acaacgctgc tgcggacagc tgaagaacaa cgcgccgcca gcgacttgcg gctggcctat
     5341 gtcgaggtgc gtgaactcgg cgatcggatg atccgtgaag cacgtgagtc gccgccagat
     5401 cgctctgaac agcagcgagt gctgcacacc ggcatcgaac acggcagcct gcagcggtat
     5461 gagcgcgccc tggtggcaat gcgcgacatc gtcgcccacg agcgcgccga acggggccat
     5521 cagttgctgg tcctcaagcg gtgggtgggt gcaggactgc tgctctctct ggtgtgcggc
     5581 tttgcactgc ttgccttgtc aagcgagttg ctccatcgcc ggttcgcgcg ccccctgtcc
     5641 gacgtcctgg cgtcaatccg gcggatcagc cagggtcagt tggaacatcg agtcaccgat
     5701 tcaggcgtgg ccgagatacg cgaactggcg ctcggcatca acaccatgtc gagcgagctg
     5761 agcgctagca ggctcaaact ggtgcatgct gaaaagcagg ccatgctggg ctccctggtg
     5821 cccgtggttg cacacaacat ccgcaacccg ctggccagca tccgtgcaac cgcgcagatc
     5881 atcgacgcgc cggcgctgcc cgcagacgtc cgcgaggggc tgcacgacat catcagcgcc
     5941 tccgatcgcc ttgaatcctg gacgcattca ctgctttcct acctgaaccc gctgcagccc
     6001 aattgccaat gggtgacatt gcaaaccgtc gtcgagacat gcctcgggct gttgtctgcg
     6061 cgcacagctg agcgcgagtt gtcgatacgc cgaatgggct gggatgctcc ggtgcgcgtc
     6121 gaagccgacg tggcgctggt cgagcaagcg ctcacgggct tgctgatgaa tgcgattgag
     6181 gcttcgccag tgcgcggtca ggtgacgctt gccgcagaca ccgacggagg catcgtgcgc
     6241 ttgtcgatcc gtgaccaagg ttctggcatg gcagttctac cgtcggccag ccggcgactc
     6301 acgatgcgca gcaccaagcc acaaggcaac ggattgggca tccccttttg ccgcaaggtc
     6361 tgcgaaggcc atggcggcca gctgaacttt gtcggacacg cagacggaac cgaagtcatc
     6421 gtcaccctgc cacagaaggc ggtacacctt gcgacctagt gaagggcgcg ccgcccagga
     6481 gcccatggcg ggcaccctgc cccgcctgag ttgcagcatc ctgatggtcg aggacgaggc
     6541 aatgttcgcc cgggccgtct gcaagcgtct cgctgcggcc ggctacgcct gccgcagcgt
     6601 gggtacgctg gccgaagccc gcgaggccct cgccgatgcg cccgacctga ttcttctcga
     6661 cctgcgcctg cccgatggcg agggcttcga gttgctgtcg cagttgagta ccgagcacgt
     6721 gcgcccaacc gttgtcgtga tgaccgccta cggtgagctg gcgccggcaa tcgaggcaat
     6781 gaagctcggt gcggccgatt acatcaagaa gccgatcgac ctgaacgaac tgctcctggt
     6841 agtgcagaag gtgttgtcga ccgcgcagtt gcgccagcgt gtcgactatt cccgtgagcg
     6901 ggagagccac tcggccgagg gcatggaact gctgggcaac agcgccttgc ttcgcgccac
     6961 ccgcacccgt ctcgagtcac tgtcgaggct gtgtgcaacg cctggcgttg ccgcacccac
     7021 ggtgctcctg ctgggcgaga ccggcacggg caaggatgtc gccgcacgct tgttgcaccg
     7081 cagcggaccg ttgagcggcc ggcccttcgt gcagatcgac tgcgcctcat tgccccgcga
     7141 actgatcgaa gccgagctgt tcggccacga aaagggtgct tacaccggag cccagagcaa
     7201 acgggtcggg ctgatcgagg cggcagagga tggaacggtc tttctcgacg agatcggaga
     7261 gatgggcctc gaccttcagg ccaagctgtt gaacgtgctg gagcgccggg tggtgcggcg
     7321 tgtcggcgca gtgcgcgagt acccggtagc agcgcgtttc gtggctggca ccaatcgtga
     7381 cctgccacgc atggtcgcgc agggccagtt ccgcgcggac ctcttctaca ggctcaacgt
     7441 gatcgcagtg cagatgccaa cgctgcgaga ccgccgcaat gacatcgtgc cgctggcacg
     7501 ccatttcgcg caaatcaccg cccgtcgcta cggcctgcaa gagccgaact tcgaggaaac
     7561 ggcgcttgac gcgctggtgc gttacccctg gcccggcaac gtgcgcgagt tgaagcacct
     7621 gatcgagcgg gcagtactcc tgagcggtca cactatcagc gcgtccgagc tggcgctgcc
     7681 ggtgcctgag gcggctgtgc cgcaatcggc cgcggccggc gaggaggacc ccacgccgat
     7741 gggtcgggag atgatccaca tgaccctcga agaagccgag cgccatctga tcgaacacgc
     7801 gctgcacctc acgcgcggca acgtgtccga ggcagctcgc cgcctcggag tcactcggat
     7861 ggcgcttcgc taccgtatcg acaagctcaa gctcagcccc gcatcgggct gaagccaccg
     7921 cacaccacgg gcgcggctgc aagcagccgt ccggtggctg ctttttgcct cggcagtaac
     7981 ccgtatcggg ttatgcacaa gaaaaatatc aatcgaatca agggcttggc gcccacgctc
     8041 tgacgctgcg ccgtggcatg catgctgcgt agtcaacccc cacgcatgtc ggggcgaggc
     8101 caatttggcg gccatgcgtc gggccagcgc gacggcgctg cacctcccca accggcacct
     8161 gtcatcactc agcgtgccgg ctcgctgcca gggtcacccg gctgacggcg aggtcaaggg
     8221 ccgggtgtga tctgcaatca attggaggaa caacatgatc aagtcagtca agcaattcct
     8281 gaaggacgaa tcgggccagt cgatggtcga gtacggcatc accctgggtg gtgtggctgc
     8341 ggtttcgtac ctcgccatcg tcgttctcgg tgacaagacg ggcgacctgt acgcctggat
     8401 ggccaaccac ctgccgggtg gcgagtcggg cgaaaccgca caggttcgcg ccaatggtga
     8461 agatggtctg gtgggtacga acgtggtcac aggtgcggat ggcatcgttg ctgaaacctt
     8521 cgcaaatcga agcgacgacg ccacgggact caccggagac tggaccctga acggcacgct
     8581 cggcacctca tcgatctacg cctctgccga tggcggctgg aacgccgact gataggtccc
     8641 ggcccagggg tggcgagggc tcacaaggcc atcgcaaccc ccaacttccg cccgccctgc
     8701 gcgggacggg ccggttcggc accaccgatt cggccctcgc attccctggc actcgccggg
     8761 ccagattttg cgatcaggtg accggcgcgg gccttcgccc ctgggggcga cgccgcccca
     8821 aagtttgctc gaaccgcccg cccatcccgc cgccatgtcg atcatcgaaa ccttgaccgc
     8881 cccggtgagt tcgttgttct cgaacggcat cggcccggcc tacctgctgg ggctggcggt
     8941 gctgagcctc gcggtggcgg tgtccgacgc gcgtgcgcat cgcattccca acgcctacct
     9001 ggcggccggg ctgctgtacg ccttgctgct gctgggcgcg ctggcatcga cccgagggcc
     9061 ggcgttcgcg ctgcgcgggc tgggcatggg cctgttcggc atgctgctcg gcggcctgct
     9121 gtcggcaccc ggcctgttcg ctcggcaact tgcacccggt gacgtgaaat tcatgatggt
     9181 gatcggcttc tttctcgggc cgatcggcgc ggtgttcgca ctcctcaacg ccgcgctggt
     9241 cggcggggtg tgggcgctgg tcctggcttg gcggcacggt ggcctgcggc tgctgggcca
     9301 taacctgaag ttcatggcgc gcagcgccta cctgtcgggc ttcaaggacc tgggctggga
     9361 cctgcgccag gccggtgcgg tgcgcatgcc ttacggcgtg gcgctggcgg tcggcgcgtg
     9421 gtcggtgatc gcctggcagg tgacgcgcag ccagagcctg cgcgccgcac tcgcgccgct
     9481 gctgggcgcc tgaggccgca ccatggcatc cgctgcgtct tccgtgtcca aagcccggcc
     9541 gaatgcggtg cagagcgccg ttgcccgcga ccggggcctg cgcgagtccc aaaggggcca
     9601 gaagctgctc gaggccggcg aaatgagccc ggccattgcc cggttccagc aggcgttgca
     9661 acacgatccg gcctgcgtgc cagccatcag cggactgggc gaggccgcct tccgagacgg
     9721 ccgctacgac ctcgccgaag gtctgttccg caaggcgctc gagcacgcac cgaacgatcc
     9781 gatgctgtgg ggctggctcg gcaccacgct gaactcgctg cgccggcatg acgaggcgca
     9841 acagcacctg ctgcacgccc gctcgctggg ccttgcaatc ggcgatatcg atcgcggcct
     9901 gggcatcgtg gcgctggccc aggggcggct cgacgaggcc gaagcgcatt ggcgacaggc
     9961 cctggcgact gattacctgt cggcctcggt gcgcagcatg ctgggcgggc tgctgctggg
    10021 tcgcggccga tggctcgaag gctggcaact gcgagccgag aacccgaccc gaagcgctga
    10081 aaagatcccc cgccccgaat ggcaaggaat cgacctgtcc cgtcgcgacg tgctgctgct
    10141 gcgcaaccag ggtctgggcg acgagctgtt ctacctgcgc tggctgccgg ccctgcaggc
    10201 ccgcggcgct caggtggtgc tgcgctgctc ggcgcggttg cagccgcttc tggccggcct
    10261 gccgggcgtg gccgaactcg tgcccgagat cgagggcgcc gaggtgccac tgcgcgcccg
    10321 ctggcaaggc catccgctgt gcgcgctggt cgacgacctg ccgctcgcct tgggcgacga
    10381 ggctgccagt gcgaccgcgc cgtcgttgcg tctgacgccc gatgccgccc acctggcgcg
    10441 actgagggcc cggctggcgg cgctgggccc ggcgccctac atcggactga cctggcgtgc
    10501 cggcaccgca gcgcaagccg acaactcgca cgagcggctg atccgcgtcg caggcggtgg
    10561 ggcggtgcgc acgcggccgc tcgacaagtc gctgccgccg caggcgctcg gcgaggcgct
    10621 gcgcggcgtc gcgggcacgc tgctggcgtt gcagcggcag ccgatgcccg gcgagctcga
    10681 agctgtggcg gcggctgcag ggcagccggt gcacgacctc tgcgacctgg gccacgacct
    10741 ggccgaactg ttggcgctga tggcgctgct cgacgaactg gtcggggtgt cgaacaccaa
    10801 cgttcacctg cgcgcggcgg tcggccgccc cgcgcgggtg ctggtgccgc accccagccc
    10861 cgactggcgc tggatgcacg agggcgaccg cagcccctgg ttccccggca gcaccgtcta
    10921 ccggcagacc ccgacgggcg actggtcgcc tgcgctggag cggttgcacg ccgacttgct
    10981 gtcccccagc ccgttggaga ttccatctgc agggccgtcc acctgcgcat ctaccggctt
    11041 acggtccacc ccaccacgca gtcccgcggc ttgtccggat cacgacctcg tgcgccaggg
    11101 cctagatctg attgccaccg gcgagcatgc tgccgccaag tcgcacttca tcggaatgct
    11161 gcaaagcgac ccgtcgaatg ccgccgcggt gcacggcctg ggccgggtgg cgcatgcaga
    11221 gagccgctac gacctggcgc tgcagctgct gactcaggcc gtggcagcag cgccggcgcg
    11281 gttcgactgg cagggtgacc tgggtctggc gcacttggcg ctcgacgacc atgcgcgcgc
    11341 gctgccactg ctgcaggccg cagcggcgtc cgaggcggcg tcggccgatc accggcgggc
    11401 attgggattc ctggcgctgc agcacggcga ctggtccggc gccgcggcgc actggcaggc
    11461 gggcctcgcg cgaacgcccg ggcaccggcc ctgcagcggc ttgctggggc gctggcacct
    11521 ggcgcgccag caatgggccg agggctgggc gctggtcgac cgtttgccca cgcgttgtcc
    11581 tgcggcggtg agcgcggtgc tgcagccctg gccagacgca gcggcgctgc gcggcgcccg
    11641 ggtgcgggtg gtcgtaaccg agggcctggg cgatgaactc ttcgcactgc gcttcgcacc
    11701 ggctctggcc tcactgggtg caagcatcga ttggctggcc gaaccgaagc tgctgccgct
    11761 gctgcgccgc ctgcccggac tcgacagcgt gatcgcgttc gagggcaccg aggcggcagt
    11821 gccccacggg gcggccgagc ccgactacac cgtgctgtcg ggccacctgc cacgactgct
    11881 gtggcagcac tcaccctcgc ccacgccccc gagcgcccgc gtcgcgccac tggccgatcg
    11941 tctgcgcgcg ctcgaggcac ggctcatcgc ttcgggtcca cggccctggc tgggcctgac
    12001 gtggcaggcc ggccaggcgc aaccaggacc cgctgccgaa cgcacgttcc gcggcccgga
    12061 cggctcggtg atcaagcaac gggcattgag caagcaggtg ccggcaagcc agttcgcccg
    12121 ctgcaccgat ggcctgccgg gcaccgtgtt cctgctgcag cgcggcgtcg atgcggcggc
    12181 gcgcgaagcc ttcgcgcagg cctgcggtcg gccggtgcac gacctgactg atctgagcga
    12241 agacctcgag gacttgctgg cggtgatggc cctgctcgac gcctacgccg gggtgtgtaa
    12301 caccaacgtg cacctgcgcg cgctggccgg ccgcacgagc gaagtgctgg tgccgcaccc
    12361 ggtgcccgag tggcgctggc tcctggaggg tgacgaatcg ccgtggttcc ccggctgcgg
    12421 cgtacaccgc cagcgcgccg atggcgactg ggggccggcc gtggcccgtt tgcagcgggc
    12481 attgcagtcg acgctgcaat ccgcgtcgaa cgattccacg aagcggttgt ccacaggcca
    12541 gacacttccg cacgcaggca ccacgctggc tgccgatggc aaggcggcag caggtccggc
    12601 ggtatcggac gttgacggcg tggtgttctg gctgacccac ggatcgatct cgctgcgcga
    12661 cggccagccg tggtcggaac tggccagtgc acggctgcgt gcgctgctgc cggccgccgg
    12721 gctggcgccg gccggtctgc ggtcgcgctt cctgagcgag accggttgcc ccgagtcgct
    12781 ctctggcaac gagccgccga cgccgggtga cacgctggtg atcggcaaga gcttcagcca
    12841 cgacaccacc aagctgctgc tgcaatccaa gaagcaaggc acacgcatcg tcgtcgacgt
    12901 gtgcgacgac catttcgacc accgcgagca cgggccgcac ctgcactttc tggtgaccgc
    12961 cgccgatgcg gtgacggccg ccactccggc gatggccgcg cgcatcgccc aagccagcgg
    13021 gcgcgacgcc acggtcatcg gtgatcccta tgagggcccg gccggcgtgg cccactttca
    13081 gccccctgcg gccggcgatg cccggccgct gcggctgtgc tggttcggcc acccggtcaa
    13141 tcacgacacg ctggccgcgg cgctgcccgc ggtggtggcg ctggcggcca cccgcccggt
    13201 gcacctggtg gcggtgacgc accgcgccca gctcgggccg gtgctgcagc ggcacaccaa
    13261 ggcagcgaag cggggggcga ccggagccgc cggcgtgctc accacccaca tcgagccgtg
    13321 gacgcccgag gcggtgtggc aggcgctggc cgactgcgac atctgcctgc tgcccacgct
    13381 cgacggcccc ggcaagtcgg tgcgcagcgc caaccgcctg atcgaggccc tgcgcgccgg
    13441 ccgctgcgtg gtggccggcc cgcatccggc gcacgagccc tttgccgaat gcgcctacgt
    13501 cggcgacctc gcgcagggcc tgcaggcggc gctggcggac ccggcggcga cgctcgcgcg
    13561 catccgcagc gggcaggagc gggtggccga cacctgctcg cccgacgtgg tgtcgcaagc
    13621 ctgggcacag gtgctacagc gccccgcgcc gccagcggca accgaagcac tctcgcccgc
    13681 ggctccgtcc cgggcgacaa ctctcgccgt cgctgtcccc gccatccctg ctgctgccgt
    13741 tcggtacccg gatccggatt cgcccccggc gccggtggcc gcgaccgcaa cagccgcatc
    13801 tgcggtgcgc ctcaaccttg gctgcggaga caagatcctg cccggctacg tgaatgtcga
    13861 cgtggtggag agccgcgccg gcaagcggcc ggacgtgctc tgcgatctgc accggctgga
    13921 accgtttgcc gacgacagcg ccgacgagat tctcgcggtg cacgtggtcg agcatttctg
    13981 gcgctgggag gtgctggacg tcctgaagga atgggtgcgg gtgctcaagc cgggcggcct
    14041 cctggtgctc gaatgcccca acctgatttc agcgtgcgag acgttcctgc gcgaccccga
    14101 ggtcgcggcc agtggcggca aggaaggcca gcgcagcatg tgggtgttct acggcgaccc
    14161 gcagtggcag gacccgtaca tggtccatcg ctggggatac acgccgaaaa gcctggcgca
    14221 gctcatgggc gaggcaggcc tggtcgacgc gcggcaggaa ccggcgcaat tcaagctgcg
    14281 cgaaccgcgc gacatgcggg tggtggcgag gaagcccgtt cgcctttcga acgccggcgg
    14341 cattcgcatc gtgcacttca agtgagtcga gcgcggcatg ggcgatagca accagctcta
    14401 ctacctgcgc cggcatgtgc cgagtgcgca cgggcccgtt ctcgagatcg gcagcaagga
    14461 ctacggcaac accgctgact ttcgggcgat ctatcgcgac gtgccctatg tcggcctcga
    14521 cctggccagc ggccgcaacg tcgatgtggt tcacgacctg acgaagggca tcggtcctct
    14581 cgcgcctgat cacttcgatc tggcgatctg ctgttcggtg ctggagcacg tagaccggcc
    14641 ctgggacttc gcgcgacacg tctcgcgcgt cgtgcgcccg ggggggctgc tgtacatgtc
    14701 ggtgccctgg gtgtggcgct tccatgccta cccggacgac tacttccgct tctcctggcg
    14761 cggcatcgaa gtgctgttcc cggacttcga atggcgcgag cgctactact cgaccaacgt
    14821 ggacgacgag ttcatttcga tcgacgagtt tcgcgacgcg gacaaccgga tgtcgctgtt
    14881 gcaggaggtc gagggtggac tgcgcaagca cctgccctac ctgatgctga acatgctggg
    14941 cacgcgccgc catgacaccc cggcgatcga ctgagaccac cccggtgcgc cgccatgcac
    15001 agccgcaaca tcgcctatct gcccgcggtc gaccaggtgc gtggcttcgc ggcgctgtgg
    15061 atcctcttct atcactcgta ccagctgctc ggcagcaggc tgcgcacagg cgagccgttc
    15121 cgcaaggaaa ccatgtggga gcgcgatgcc ggtccgctgc tggctccgct ggtcgaaggc
    15181 catacggcag tcgcgctgtt catggtcttg tcgggattca tcttcacctg gggcgccatc
    15241 ggccaacgca tcgactacct gcgcttcatc ggcaaccgat tccttcgcat ctatccgctc
    15301 tacctggtgc tgctcatggt ggcgttggcg gctcgaccgc gcgccttcga actggatgtg
    15361 gtgctggcca tgctgctgcc gttcgccgat ttcgtgcgcc ccggcaccgg tccgctgacc
    15421 ggaatgtcgt gggccatcgg cgtcgagttc cagttctatc tcctgtttcc tctgctgttc
    15481 gcattggcgc gggacaaccc ggcacgcctg atcgctggct ggatcgcgtg tgcggtgctg
    15541 ttgcggctgc tcgcagtggg tctgggaggc agcgcgtacc acgtgtctta ctggcatctg
    15601 gccggtcgca tcgaccagtt ccttctcggc atggccgcgg cgttctggct cgcacaggct
    15661 cggccagagc cgcgtcgctg tgtgctgctt ctgtgcatgg ccgtgccgat gagcgtcgca
    15721 cttctgcacg cctttcaccg cggcggcggc ctgccggtgt cggctccgtg gaaaacgatc
    15781 tggccggcca tcgaaggcgc catgtgggcg ctgtgcatcg ccggctggat cgggctgcgc
    15841 ggcgcaggcc agcgctggcc agcacgagtg ctgcagtggg tgggtacgcg cagcttctcc
    15901 atctacctgc tgcattaccc gttggtgcaa ttgctggctg ctcaccctgg cctttggtgg
    15961 gcgccgacag gcgagtggcg caccgatgca ctgctcacgt cagcctgcgt cgtcttgcct
    16021 ctgagcctgc tcctgtcgag cctgacgtgg tcggcaatcg agaagccctt tctatcgctg
    16081 cgccgaagtt acctgacccc gctcgacggc ccccccagcc cgttgacggc gcacccgaac
    16141 accgcgtctg tcgcgcgggc accttgagga gacacacgcc atgatccccg tctacatcgg
    16201 cttcgacccg cgagagaccg tcgccttctc ggtgctgtcg cacagcattc atcgccgggc
    16261 gagcgagccg gtcgccatcg cgccgctgat gctgtcgcag ctgcatcgcc agctctggcg
    16321 cgagcggcat ccgctgcagt ccaccgactt ctcgttctcg cgcttcctgg tgccgagcct
    16381 gtcgggctac gcgggctggt cgatcttcat ggactgcgac atgctggtgc tcgaggacat
    16441 cgcccgcctc tggtcgctgc gcgacgagcg ctacgcggtg atgtgcgttc agcacgacca
    16501 ccggccggtc gaggaccgga agttcctcga tgcggtgcag acgaagtacg cgaagaagaa
    16561 ctggtcgagc gtgatgctgt tcaacaacgc gcgctgcacc gcgctgacac ccgagtacgt
    16621 caacaccgcc aacggactgg agctgcacca gttcaagtgg ctggccgaag acggcctgat
    16681 cggcgcgctg ccggcgcgct ggaaccacct ggtgggctac gacgcgcccg atcccgcccc
    16741 ggcgctggtg cactacaccc agggcgggcc gtacttcgac gagtacgccg attgcgagca
    16801 cgcggggcgc tggcgtgccg agcgagagtc gatgttgcgc tgcgagcagc gcctgcccgc
    16861 gcgcgccgcg gcctgaaccc gtcgacgccc cgatgccccc ggcaacccct acccgctcgg
    16921 cccggcccca gcgcggtcag gccctgatcg agttcgcgat catcctgacg ctgctggtgc
    16981 tgttgctggc cggcgggctg gagctgggca ccgcggcgat caacagcaac caggcgcgcc
    17041 aggcggccga acagggtggg cagcagtgga gcgaggtggt cggcgccagc ggcctgtatg
    17101 tcgccgccgc atccagcccc tacgcgatcg gcaatggagc ggtggtgccg gcgcccgcga
    17161 cggtgggtct cggcgaccac accccagcga acttcgcacg gccggcctgc gacgacctgg
    17221 cctgcaccgc gcccagcaac ggcctgaccg gtccctcggc cgcgctgaat ccgccgatcg
    17281 acccggccac gggcgcgttg actggcgacc tgtacctgtt caatccactg ccgatcgacg
    17341 tgaccagctg cgtggtcaac gacgcggtcc agtacaccga ttgcgtgaac cgcatcttcg
    17401 acggctgcgg gccgccaacc tgcccggccg cagtggccgg ccttccgcgg ctgaatgcgg
    17461 ccctgcgcgg cctctacacc ctgcgctgcc tgaacgccgc cgacgcacag gtggtgtgtc
    17521 gcagcgtggc aagtggcggc accgcggtca cctggctgct gcgcttgccc ggccggcacg
    17581 acgtcgccgg cgacgacacg gttcggctgg ccctgctcga cgacacctcg aacgtgaccg
    17641 ccgaggtgcc catcttcgag atcgagtgcg cggcggcggg cggcgccttc atcgatgccg
    17701 gcggcaatcc ctgcgacacc caggcggcgc cgggcgaggt gtgctggacc gcggcaggca
    17761 ccgccctggc ctgccgagtg cgggtgaagg tgcgctaccg ccaccacttt tatgcgctgc
    17821 tggcctcggc cttcttcgac ggcgatccgc tgccagtgtt gaccgctgcc gcactcgacc
    17881 tcggcgaggt caacgcggcc ggcgtgccgg tgggcggcta cggcgcggaa gtcacggcac
    17941 aagagctggc atctgcagcg cccaagtatt tcaaggtgcc gtggcgcacc ttccagggct
    18001 gcgggtcgac gcgtaccgtg cgcgacggca ccagcggcct gcgcggctcg accgccaact
    18061 gcaactgaac tgaacaccac cgggagcaaa cacgatggtc accaccgtca tcactgcacg
    18121 acagcgccgt gcccgcaacc aacgcatcgt cctgttgacg gcggccgttc tgctggtcgg
    18181 cggcggcctg tggtatctgc tgcgcggcga ggagcgagcg ccggccgccg agaagcagcc
    18241 gctgggcacg attgcggtgc cagtggccgc gcgaccgctg cccaaggggc gcgagctcga
    18301 ggggccgggc ctgatccggc cgatgtacct gcggcctgaa caggtgccgc ccgcggccct
    18361 gctcaagacc aaccagatcg tgagccgcgt gacccgcgaa tcgctcaagg ccggcgacta
    18421 cttcagcgag gagaacctga cggccgccgg cgcgccgccc ggcttctcgg gtctggtgcg
    18481 gccgggctcg cgcatcgtgg tgatcgagac cacccgcatc gcgggggtga tcggctacat
    18541 gcgcgaaggc gacctggtcg acgtgatggc gctgtcgcaa tcggcggggg caggagcacg
    18601 gcccggcggc ggccccacca tcgaggcggg cggaacccag cccgggtccg gaggacaagc
    18661 ggccggaagc ggagcggcgc gaggcggcgg gggttcgctg ccgggactga cggccacgct
    18721 catagcgcag ggtgcgcgcg tcgtgatcgt gcccaagccc ggcaagaatc agtttgcggc
    18781 gttggaaatg gcacccgagg acgcacacct gttgacgatg gtgatgtcga ccaaccaggt
    18841 gctgcacttc gtgtaccggc ctttcaacga cgactcccgc ctggacgccg acagccctgc
    18901 cttggccgaa acacggccgc cgcgcgactc gcgcaccatc gaaatgatcg aaggcgtaaa
    18961 tcgctcggtg ctgcgcacca ccctcaactg aatccacagc gcagaggacc cgtcatgaaa
    19021 acgaaattcc tgcgcgccgt gcaagcatta ctggtgctgg ctttggtcgt ccttctgatc
    19081 atcgcgatgg caaagtaggt atgccggcga ttgccaattt gactactcgt gcccacaggg
    19141 tctcgccaca agccggtctc cgaccgcgac ggtggcggcg gtaccagccg caagttccag
    19201 cgccgatcgg gcgcccttta cccacggcga cagtttccat ggggcaagcg ccggcaccac
    19261 tcgcaagacc ttcatgtccc ggtcgaggaa gatcacgtcg atcggcgcac ccatgaaatg
    19321 ggtgtgcacc tgctgggttg gcacgatcca cagcccccgt ccgggttgaa gcggcggatg
    19381 tccgatcagc ccgcgcaggc ggctgaaata ccgattggcc acaagtacgt cggcagccaa
    19441 ggtcgaggca ccgtctgctg cgtcgcagaa catcgacacg cgcttcatca tgtagcggtt
    19501 gcaatcatcc cggcctgctg gcgatgatcg actccatcac acggtagatt cgcagcagcg
    19561 atggcaggcc ggtcacgatg aacatcgcag gcaggatggt gaccagcatc ggaaacatca
    19621 tccggatagg caaccgttcg gcctgccgct gcacctcggc ctcgtactcg cggaacagcg
    19681 tgcgcgcgtg gtgccgcaac accgtggcga tcgagctgcc ggtctggtcg gcctgggtga
    19741 ggctggacac gatctcggcc acctgcacga tgttgcagcg ctcgccaaat agtcgcaatg
    19801 cttcgtggcg gcgccggcct cggcgcattg cctccagcat caccgacagc tcgaagtaca
    19861 gcgggtggcg aaggtcagaa tcacgcacca catgctgcag agctgtctcg aaggcgatgc
    19921 caccttccat gatggccgcg acgagttcga gcatgtccgg taaggccttt cggatgcgtt
    19981 gctcgcggcg ccgccgccgc gagtaaagat aggccatcga acccagagtc atggcaagcg
    20041 atgaggcgac cgacagggtg atcgacgcat cgagcttgcg cccctgcgaa taggcgatgc
    20101 cgaagcctgc agctcccgcc agcgcagcca ccagcacgcg gccgcgccag ctgcctacga
    20161 acagcgcgaa gtggtagcca agcctgtccg taccaaccag cccgtagtcg cctgccgtca
    20221 ggtcgtcgcc tgcccgttcg cccatcaggt cgaggccgaa tgacaggcgc tcgcgaagca
    20281 ccgccagctg gctcgcgcgc accacgtaga gcacgccgag accgatcagc gcagcaatgg
    20341 acagcacgac caaggtggaa ttcatctcaa acgtccaggc gtgcgagtcg acgcatccag
    20401 aagaagccga gcaccagcag cgcgccgccg gcccacaatg tgttgacccc taaaggatcg
    20461 ttcagcatga agctgaatat gcgcgggttg cggtagtagg tgaacagcat gaacagcagc
    20521 ggtagcgccg tcagcacgta acccgccaag cgcgcctgag cggtcagcgt gcgcacgcgc
    20581 aggatcatgc gctcgcggga ttgcatcgac tcgaggatgt gctcgagcat cgaggtcatc
    20641 tgcccgcccg cttcccactt taggcgcact cctgtggcca gcagctcgca atgggcgccg
    20701 ccacagcgga tcgcccaatc ccgccaagcc tgggtcggag tgacaccaag ctggagcgcg
    20761 tcgagcaatg cctgcagcgt gccgccgagc ggctgaggcg agatccgcgc ggccgtctgc
    20821 agcgacgaca gcggggtacc accggtgcga agcgcagcca gcatctgtgc aacaaatgcc
    20881 ggcaactggc ggtcgatgcg ctgcacccgc tgatcagcgt aggcatcgag cgccagccac
    20941 atcagcaagg gcacggccac gagggctgcg atcggcagcc agagggtgcg ctggaagacc
    21001 aacgccagtg ccaccgcaac gctggtggac accaccacga cgaacagcgc gcggcccagt
    21061 ccgagcggca ggttgctgcg caccagcctc tcggcgaact gacgggtcag cggcgaacgc
    21121 tcgagcaatg cctgcaactg ctcgatctgc agcaggtcgg ccgcgtcgcg ccacaggctg
    21181 ccgtcaggag attcgtctgg cgcctcgatc gacaggcgct cgcgcagtcg ttcactgcgc
    21241 accgattcgc ggtaggccag cacggcaatg gccgcagcag caacgatcag caggccgata
    21301 agcagttgca tggcgcggcg tgcgaacggg gtgcgatcac tggtcactca gtcgctgaaa
    21361 agtgccgggt cgagcggcac cccgcgctcg aagaactcgg gcgcgctgga cggcacgatg
    21421 ccgcacggat acatgaccac ccggctgccg tcgcgccgca cggtgaagac gtcttgcgtc
    21481 agcaccgtgc cgttgtcgac atcgacgact tcggtaatgt gggtcacgcg acgcgaacca
    21541 tcggcaaagc gcgccagatg cacgatgaag tcgactgccg aggcgacctg ctcgcgcagc
    21601 acccgttgcg gaaggtcgac gtcggccatc agcatcatgg tctcgagtcg cgacagcgtg
    21661 tcgcgcggac tgttcgagtg cagcgtggcg agactgccgt cgtggccggt gttcatggcc
    21721 tgcagcatgt cgagcgcctc ggcgccgcgc acctcgccga cgatcacccg gtcgggccgc
    21781 atgcgcagcg cattgcgcac caggtcgcgg atgctgaccg agccacgccc ctcggcgttg
    21841 gccggacggg cttcgagcgt gatgatgtgc tggtggtcga gccgcagctc ggccgtgtcc
    21901 tcgatggtga tgagccgctc gccttcaggg atgtgctgcg acaggcagtt gagcatcgtt
    21961 gtcttgccgc tgccggtacc gccggacacg atgatgttca tgcgggcttg cacggccagg
    22021 cgcaggaact cggctgcggc ggcgctgagc gcgccgttgc caaccagctc gtccatgccc
    22081 agtatctcgc gcatgaagcg acgaatcgac atcgtgggcc cgttgactgc gatgggaggg
    22141 atgatcacgt tgacccggct gccatctgcc agacgagcgt cgcccatcgg actggattca
    22201 tccacccggc gtcccacgcg cgagagaatg cgctcgacgg tgagcatcag gtgctcgttg
    22261 tcgtgaaacc ggatgcgagt gagttcaagc acgccatggc gctcgacgta aacctggtgc
    22321 gggccgttga cgaggatgtc ggagacggtg ggatcggaca tcagcggaga cagcggaccg
    22381 aggcccacga cttcgtgcag cagatcgtcg ctgagctgcg cgcgttccat gcgcgacagc
    22441 ggcagcgtgt cgcgcgccag cgcgtcgtca aggtactgct cgattgctgc ctggatctga
    22501 ggcatcgtgg cgttggccac cgcctgcgga ttgaggacgt ccagcaaccc gcggtgcagg
    22561 cggtgccgca cctgagagta ggtcgcgctg ttgacattga ctgcagtgct gagcgcggct
    22621 gtcgccagtg aacccgaggc ctgctgctgt tcgttgatca ggcgcgcgtc cttctggccg
    22681 ctggcgaggg cgcgcattcg cgagcccagg aaatcggtct ggctcgcggt gttgcgtcgc
    22741 aacgtgtcac gcagcgtcgg cgcgtgctcg gggatgccgc gatcggcgtc gtctggcacg
    22801 ctcaattcag taacccctca cgaatcggct gaggcgctcg atcaggctgg gacgaaagac
    22861 cggcagcctc tcttgttcgc cgatcagccg atcggccaac agttcgagtt cgtcggagag
    22921 cggcacgtta gggaacatct cgacgacggc acgaccgctg ttggccgcga tggtggcatg
    22981 gacgtcatag ggcagcacgc ccgcgatggg ccactcgagc gtttcggcga ccagatcgat
    23041 aggcacgttg cccctaaacc gggtgtagcg gttcagcacg accagaatgc gctctggacc
    23101 atagcccgcc tgcttgagta cctgcaggtt gtagcgcgcg ctgcgcagcg cgggcacgac
    23161 gggttcggtg acgagcacga tctcatcggc caggtccatc agtgcgaggt tcaagctcga
    23221 cagcacggga aaggtatcga cgatgacctg ctcgtaggcc gacttggcca ccagcaggat
    23281 cttggatagc tggtcttcct cgaagtcgag cccgagcggg ttgtcggatg aatgggccag
    23341 cacctgggct ccgctcgagt gcagcgtcat gaactgctgc aacatctcga tgtcgaggcg
    23401 gcggtactgc tcgatcgcat cgcgcaggaa gtgctcgggc cgcaggtcga gcatgggcgc
    23461 cacgttgggc gcctgcgtgt tggcgtcgat caccgccacc ttgttctcgc cgagcttctg
    23521 cgcaagcacg accgcgaggt tgcagctgat ggtggtcttg ccgactccac ccttgctgga
    23581 gaagaccacg atgatgcggc ctcgcgcgcc atcctgcagg ggctgaggct ccatctgccg
    23641 ctgcgccttg cgcagcacag cgtggatgtc gtcctcctgc gggggctgtg ctatgaactc
    23701 gcgcgcgcca gcccggatcg cccgcgacat cagctccgtg ctcgacttgt cgacgctggc
    23761 caccaccagc ctcgcgtagg gcacggcggc gagttgctcg atggcgatca tgccggcttc
    23821 gacgttgcgg gtgaggtcga agaacacgac ctcggcctcg aattgcttgg ccagttccag
    23881 cccctgtccg atgctgtcgg acagtcgcat gctgaccagg gtttcgccat accgcaggca
    23941 ggccgcctgc accggctgac ggaactgctc gtgcgcgctg atggcgagga tgcgcatggg
    24001 ctgcgtgctg agtgctgagt gctaagtgct gctgcgggat gcggtagaac cggctcagcg
    24061 cgcccggctt gcaccgtagg cgcgcacggc gtcgtagagc gtgagggggg gggccgtgtt
    24121 ggccggagat tgcccgagcg aatcgtcgac gccgagcaag tagtcccagc cagccaggac
    24181 acgtgcatcg gcaaggcgaa ccagcttcaa gctgacccag gtgccttgag caccgcgcgc
    24241 gctggtgcca accaccgcag cctccgcatc gtggctgcgg gcaatctcgg acagctcgcg
    24301 cgacagcatc agcgccccgg tggcgcgggc gacgaacagg ctgccgcgca gcttgacctc
    24361 gcgcacggcg aggccgcgct gggccaggcg cccggcgacc tgctcgctga ccagacggcc
    24421 gagcgccgtc gagcggtcca ggtcgtcctg cgacaccacg gtggcgacga cgaccgtgcc
    24481 gagcacctcg ggctgggtag cgtctgtggc cagcaggcga tcggcggcct cgtagttggc
    24541 gcgcgtcagc ggatcgtcga tggcgtcggt gtcggccgga tgcgccgccc ccccggcttc
    24601 ggccgcgaag ccgggcgcaa ggcctgtcgc catcgagccg tgcggcgggc gcaggtgatg
    24661 acagcccgtc aacgccaggc cgagcgccac gccggccgcg acccggcgca gcgtcgaacc
    24721 ccggttcatg gctttctccc ggcgagactg cccaccacct tgagcgacac gctgcggtcc
    24781 cgcgccggca ggtagagcgc gaggtcggcg tcggcggcat agaacagcag gctgcgccgc
    24841 agaccgggcg ggccgccggc atcgagcgtg gcggtcagca ccagctcatg gcgtggcggc
    24901 gcggtggaca gcgattgcgc gccgatgtga ccggcgccga acgggatcag ccgcgtgtgc
    24961 agcgacagca ctgggccccg cagctcggtc gcgcggggat cgcccgggcg ccacaccgcc
    25021 acgccctgct cgtgcagttg cgtggccagc aggtcgcgca cggtgtcggt gaacaccgag
    25081 ggctcggggg tgtcgatgcg cagcggcgca ccaccaggcc cggccttcag cgccgcggcc
    25141 acgtctgcag ccaggccccg cacgcccagc tcccaatgcg tcagcgcgtg cagggccggc
    25201 tgcggcaggc tcaccaccgc cggcggcacc gggtccagcg gcacccgggc acagcccgcc
    25261 agcagcagga acgcgctgca caacacgacg ccagcgaccc tcgcaccgat gtgctggccg
    25321 gtcccggccg gattcgaaaa caggctgttc atggatcact cctgggctga ggcgggtcga
    25381 tcaggcgggt ggtgcgggcc gattcatggg cttgcttgaa ccggttgatc tcgtcctgca
    25441 gcttcttgat ctgctcgggc ggcgccaggc gcagggcctc ttcatagcta cgctcggcct
    25501 caccgacctt gccgcccgcc tccagcacgc gcgcgtggag ccggtaagcc acccagactt
    25561 gcgggtcgag ttggatggcc aagccggccg aacgctgggc ggggttgtgc tgccccagac
    25621 gcgacagcac ctgcgccagg tcgccgtgca gcggcccgaa ctcggcgcgg tgagcgatgg
    25681 cgctgcggaa atcggcggcg gcggcgtcga gcttgttgag cagcaggctg cattgcccca
    25741 gcgcgcgata ggcgcggctg gcctcgagcg cgagcgcctc gtcggccgcg atcgactgcg
    25801 ccatctgccg ggcgcgctcc atcagcggcg ggcaggcgcc gctggccttg tccggcgcga
    25861 gcgcggctgc cgcctcgatg gccgcgagcg catcggcgat ctcgcggtcc tgcgtcgtct
    25921 tcaggtacca caggccggca gcggccagcc ccaacagcac gagggcgatg ccgtagagcc
    25981 cggcgcgccg accgcgcagc caaccaagcg ccatcagcga acctcccgag ccgccgtgac
    26041 cggtgccggc agcggcacct tgcgggcggt gatggtgatg atgagctcgg agcgggcgcg
    26101 ccgcttgttg ctcgactgga agagcttgcc gaggatcgga acgttgccga tcagcggtac
    26161 cttctcgaga cggtcgctgt cttcctgcgt cagcaggccg gccaccgccc aggtctcgcc
    26221 gtcggccgat tcggtgatgg tctgtacagc ccgcttgctg aaggacggca cctggttgcc
    26281 accggacagg aagctgcggc tgtagtcggg ctgggtgatg ctctggtcga gcacgaggct
    26341 gatgcggccg tcctcgagca ccgtgggcct cagaaacaac tggatgccga actcccggaa
    26401 ctcgagcgtg ttctggttgg tggtggtgga gccgcccacc gccgagttgg ttgaaatcga
    26461 ggtgttggcc accgggatcg ggatctcgcc gccgaccagg aagcgcacga tctcgccgct
    26521 gaccgcggtg agcagcggct cggacaacgc ggtgccggcg cccttctcct cgatcgcctg
    26581 caggaaggcg gagatcgaga ctcggttgct gcggaagaag ccgacgatgt tggccgtgcc
    26641 gacgtcaatg gcattgttgt tgccagcgcc gctgccgccc gatccacccc cgccgatgtt
    26701 ggtgctggag cccgacgcgg acggcagggt gtcgaccacc ccaccggtga ggttgccgat
    26761 ggacacatgg ctgccgtcga gccgccagtt gaagcccagc ttgtcggtcg cgtcgcggtc
    26821 caccgcgacg atgcgcacct gcacctcgac gcgcggctgg tcgcgcacct cgatcaggct
    26881 gagcgcccgt ccgccagcga tcgacaccgc actggagcgc gacaggttga gcgcgatgtt
    26941 gcccttggcg ggcgactcgc tgatggcgat gccggcgctg cccgcgctgc ctcggttgct
    27001 gctgctgaag ccgctgctcg acccgccccc gaaggcggag gactggctgc gccggttacc
    27061 gccctcgcca ccttgcccgc cggacccgac gttgctgttg ttggccacca gcacgccgcc
    27121 ccggtcgctg accgggcgga aatcgccgct cgcggtgccg acgccaacgt agctgtcgat
    27181 cagggtcagg gcgcgcgtga gctggatggg cgtatcgacc ttgccggtca cgtgcagtac
    27241 ggtgttcacc tcggtaaccc gcaggctggc atcgatggcg gccagcatct ggcgcagctg
    27301 cccgtgcggc gcgacgaacg actcgtcgga ggcgagcaga tcgatgatct gcggcgcgtt
    27361 ggcgtcggtg gtgcgcaccg ccgagatcac cgactcggtg ttcaggggca ggcgcgcctg
    27421 catcggccgg ccgccggccg gcgtgcgctg gcgcgtcgac cccggcgtct gtgcgagcac
    27481 gttggccggg ccgacgtcgg tgctgatgtc ctcaaccgag gcgtcgtcgt cgcggctcag
    27541 caccagaggc atgtcgagca cggtgtagcg ggtgttgccc acgaagctca gcgcagtttc
    27601 caaggcgcga cgcttcagct gctcggtgcg cacgaggccg ctgatcacga tggcgttgcg
    27661 gttcggatcg gaggccacct tgatgcgcgg atcgatcatc atcaactggg cgcgcagcgc
    27721 gtcggtgttg tgcaccacgt gcacgaggtg gctctcaagt tcgcccttcc tgccgaagaa
    27781 catcagcgag gtcgcgccaa acttggctgc attgatcagc acccgcctcg gagtcaccgc
    27841 ggtagcgcga gcgatgtccg actcacccac agcaatgctg gcgacatcga atggaacctc
    27901 gaccaaccgc gagtgcgaac gcagcacctc aaaagtctcc gcgcgggctg caccgaagga
    27961 taggatcgcc cccacggaac acaaccactt cgcaaggcgt cgggtagggc ggaacaagcg
    28021 agcagacctc atgggcaatg cggatgcgcg aacggaaaag cagcagagag cttgaccagc
    28081 gggtcggggc ccagagcgct tgcgagtcgc aacatccgag ccatcgggag caacccgatg
    28141 gacttacccg gctcgacggg ccgagcgaag caattgaccc gggcaatgca cccgcagtgc
    28201 tcggatgagg tggctgaatt gaaccgtcga ggctgttatc agccaaacgt cccgaccgac
    28261 tgattcagcg gtccaccggt ggccagcagt caagtcagcg gcaggtcact tgatctcgat
    28321 cgtggcacgc atgcctgatt cagtgtgcgc ccgggtcgac atcacatcgt cgaagcgccc
    28381 cgtcctcacc ggcaaaaacc accactcaag aaggctgccg ggcttgagtt cgaaacgccg
    28441 caccggaccg tagacctcgg ccaagacctt gccatccgga ccgagcgcga ccaccttgcg
    28501 cgaatagacc gagtcggcca gtccctgaga accgaagtag taggcgttgg cgctcggatt
    28561 ctcgagcctc aacacataaa gcttgcctgt ctcgaagacc agcgaatccg gcgtgaagcg
    28621 atgctcgcca ccgacctgcc caagcttcag ctcgacgatg accggctctt gccgcgtcag
    28681 atcgccggcc gcgccgactg ggccacccca caggcacagt gccgcacacg acaacaagaa
    28741 gcggtatttc atcgaaggct actttcgttg acagaccaag ggagtcggaa tgctacggct
    28801 gagcccgcaa ctgcactgac ggtgaggact tttggcccag tccacgaacg tgtcgacatg
    28861 acttgatgcc ttccccgaac agagcgtcac tggtgtggcg ccgccatacc gcggcgccac
    28921 accaggagat gcaatgaata ccaaggccac tcgcaagacc acccagcccg ccagcaaggc
    28981 agccctgccg gtcaagacag cgccaccagc cctcaaggcc gctaagcgca ctgtccgcac
    29041 cctcaagact cagcccctcc cggttgttgc ggttgccgcc cccccaggct ccaaacaatc
    29101 ccaactgctc gccatgctgc gctcgcccac cggcgccacg atcgagcaga tgaccggagc
    29161 caccggttgg caggcgcact cggtccgcgg cgtcatcagc ggcgtgctgc gcaagcggct
    29221 gaatctcgac gtggtgtgca cgccggatcc ggccggcggt gctcgcatct accgggtcgc
    29281 ggcggcatga ccttgtctgc gttgacggag ctgccccgga tggaccgccc tgccctggcc
    29341 ctgcgctggg agagcacctt cggtgtccct gccccgaaga gctgtcaggc cacgctgctg
    29401 cgacaggcgc tggcctggca ggtccagatg tcggctctca agacccgggt cggggttcgc
    29461 gaagccgatc gcggcacccg ggccctgcga acctcctcat cgtccagtgc catcgctccg
    29521 ggcacccgac tgctgcgcga gtggcagggc cgaacccacc atgtgactgc tgttgttggc
    29581 ggcttcgaat acgacggccg ggtctggcgc agcctgagtg cgattgcccg ggccatcacc
    29641 ggatcgtcct ggtccgggcc gctgttcttc ggattgcggt cgtgacaccg atcccgtgcg
    29701 cggtctacac ccgcaagtcg tccgaggaag ggctcgagca gggcttcaac tcgctggatg
    29761 cgcagcgaga ggcctgcgag gccttcatcc
//
LOCUS       DAOORV010000027        29469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1828_length_29469_cov_8.929625, whole genome shotgun sequence.
ACCESSION   DAOORV010000027 DAOORV010000000
VERSION     DAOORV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29469
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_29469_cov_8.929625"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="secD"
                     /locus_tag="PKV56_05585"
     CDS             complement(<1..1779)
                     /gene="secD"
                     /locus_tag="PKV56_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOX67319.1"
                     /translation="MNRYPLWKYAILLAALVVGLIYTLPNLFGEAPAVQVSSGRATLK
                     IDSSVATRVEEALTKSGLQADFVQFEGNSVKARFADTDAQIKAKDAIVKALNPDPADP
                     SYIVALNLLSRSPHWLASLHALPMSLGLDLRGGVHFLMQVDMKSALTKKAESLTGDVR
                     LMLRDKNLRHAGIVREGNAVVIRFRDRETLTAARQLLEDELRDLQWTEVPDGTDIKLA
                     GALKPEASRRVQESAVKQNITTLHNRVNELGVSEPVIQQQGIDRVVVQLPGVQDTAKA
                     KDIIGRTATLEVRLVDESTEAAAAAGGTGPVPFGTERYTERNGAALILKRQVILTGEN
                     LTDAQAGFDGQTQEPTVNLTLDAKGSRIFKDVTRDNIGKRMAILLFEKGKGEIVTAPV
                     IRAEIGGGRVQISGRMTTTEAADTSLLLRSGSLAAPMDIIEERMIGPSLGADNISKGF
                     NSVLYGFAAIAVFMCLYYMLFGIISTLALAFNLLLLVSVLSMLQASLTLPGMAAIALT
                     LGMAIDANVLINERVREELRSGASPQSAIHNGYERAFTTILDSNVTTLIAGLSLLAFG
                     SGPVRGFAVVHCLGILTSMFSAVVFSR"
     gene            complement(1876..2208)
                     /gene="yajC"
                     /locus_tag="PKV56_05590"
     CDS             complement(1876..2208)
                     /gene="yajC"
                     /locus_tag="PKV56_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOX67320.1"
                     /translation="MFISQAFAQGAPAATSSPDMLGSIGSMLPLILMFVVLYFVMIRP
                     QMKRQKEHKAMIDALAKGDEVVIGGGVLGKVAKMGETYLGVEVANGVEIQVQRSSVVQ
                     VLPKGTFK"
     gene            2478..4217
                     /locus_tag="PKV56_05595"
     CDS             2478..4217
                     /locus_tag="PKV56_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOX67321.1"
                     /translation="MRAALGAMALAAVLSLAGCGGGGGGSDATASNPDSSGTTPPPTS
                     ATGGSAPARPAPEDRPQSTDAARLLTQATFGITSVGEIEAVRTDGYERWLWTQFNTPA
                     ALHTRYLDAQKGREKNRKATEEMSYEAVWQQWLTGADQLRARTAFALGQILVISNIAP
                     DIRPYAMSSYMDLLNRNAFGNYRTLLREVTLHPAMGYYLNMIESEKDDEVRGIHPNEN
                     YAREVLQLFSIGLVQLNIDGTPKVGTDGKPLATYGEAQVKGFAKAFSGWTFASQNPSQ
                     PDSFHDANEELDANWTTPMRAFASAHSPEPKALLNGVTLPAGQTPEKDLEDAIDVIFN
                     HPNVGPFIGRQLIQRLVTSNPSPAYIARVARTFNDNGSGVRGDLKAVVRAILLDPEAR
                     DVSLAAGDRYGKQREPVARFASFLRALGAKSRNGINSIHYLDSADNGLGQSPLLAPSV
                     FNFYSPNFRQPGAIARAGLFSPEFQITTETSVVGSLNFFADLFNSEGYGDGNSRLVLD
                     LKPLAALAATPSALIDRLDALFFAQQMRQSTRARLTRLLESLPGTSEYELSQRVKAAL
                     IVVSLSPDFVIQK"
     gene            4229..5575
                     /locus_tag="PKV56_05600"
     CDS             4229..5575
                     /locus_tag="PKV56_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOX67322.1"
                     /translation="MPSSALRRRQFLKLAGAATASGLGLGTLAHLSASAQSVGDYKAL
                     VCLFMYGGNDGNNLLVPLELPAYDGYRRARTNLALDRDALLPIAPTNTSGARYGLHPA
                     MAGLQGLFTQGKAAMLANIGSLAVPTSKAQWEARSVPLPDNLFSHSDQQAQWQSAIVD
                     APPRSGWGGRMIERLIAAEIANRGYGLVSMAGGNLWENGDRSLAAYKVSSAGRFGFDF
                     YDASGGDPLSAAISDTLSEPRSHLLEQAWTDVVSRSIDNQRILTQALSGSELTTVFPG
                     TGLGQQLQMIARLISARSRLGLSRQCFFCSIGGFDTHGDDQLQRQTELLGEISTAVTA
                     FYEATVALGVQNDVALFSASDFGRTFQSNGQGSDHAWGSHHFVIGGAVKGGQIVGRYP
                     ELIVNGPDDAGDGKWIPTTSVDQLGATLGRWFGAAASLNEVFPRLSNFDADLGFMT"
     gene            complement(5606..7165)
                     /gene="malQ"
                     /locus_tag="PKV56_05605"
     CDS             complement(5606..7165)
                     /gene="malQ"
                     /locus_tag="PKV56_05605"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOX67323.1"
                     /translation="MNLTERASGVLLHVTSLPGPHGSGDLGPAAHHYIDWLHDAGQRL
                     WQVLPLTPIGPGDSPYQSVSAFAGSPLLVALEPLVERGWLAADALAAAVVEADFDASR
                     VDYAKVVPWRDARLREAAAGFASRACTADSQAFSDWSRAQSHWLDDYALFMALEQSQS
                     RDGGWRAWWDWDSPLARRDPDALAAASAAQTDELFFWRFVQWCFDTQWQAVRAHARRR
                     GVALMGDLPIFIAQHSADCWARPDLYWLDDAFQPTVVAGVPPDFFSATGQRWGNPLYR
                     WDRMADEGYAWWIARVRRALDQADVFRIDHFRGFAAYWEIPADCPTAIEGHWVPGPGR
                     PLFDAIEQALGVLPIVAEDLGVITPDVESLRDGLGLPGMRILHFAFGGDAENDYLPHR
                     YAANTVAYTGTHDNDTTRGWWQSASAAERAYAGLVFGLDDCDAAEADTRVAWAAIRAA
                     SASVANLSVFPMQDVLNLDASHRMNTPGTATGNWTWRFDWPMVGAQPAPRLRRLSAAF
                     GRCPFGPLASD"
     gene            complement(7162..8166)
                     /gene="glk"
                     /locus_tag="PKV56_05610"
     CDS             complement(7162..8166)
                     /gene="glk"
                     /locus_tag="PKV56_05610"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOX67324.1"
                     /translation="MASKMAMHPAGAPWLVADIGGTHARFGLVVGPGRAVEHVQRLRA
                     DEHANLVDAARCYLASVASLCAAPTCACFAVATPLDGDEVIFTNSRWRFSRNALQRDL
                     ALDHLQLLNDFEALALALPRLASDGLRAHAAAPEPHGTLAVIGPGTGLGVAGLLETAA
                     GWQPIPCEGGHVTLSGADDFESAVLSFARRELEHVSAERLLSGIGLPLLHRAVAAVSG
                     RPADSLVTEVIIQRGIGGIDAGCAQTLSVFCAMLGSFAGNVALTFGARGGLYIGGGIV
                     PRIAELFFASAFRERFEAKGRYRGYLQAIPTALILDPQAALAGAALALEMKQAGEGVE
                     "
     gene            8380..9918
                     /locus_tag="PKV56_05615"
     CDS             8380..9918
                     /locus_tag="PKV56_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX67325.1"
                     /translation="MTALTNALRRAAHHRVVALGICTVMLTGCALQRHEPRALDPAVS
                     QSAQLSRRADDATWREAMSRLGVDTTIWPLPAWDAPELSAFALASHPELAVARTEIAV
                     AQSAGQTARQRANPSIDLSLEHHSAPGQSDSPWTIGIALDSLVGEWLLGQSRRAALTE
                     LADARQLEAVERAAQVAWQLHRRVRDSHRALHDAEKQSALADEMLALQREESVLWQRR
                     MTLGAAGAPEVLPAQQRQAVARQRAHQARLAVDQARVALAQAIGLPLEQLQHMTIRFD
                     SLDQPQPDQRAITESATASAPVLEPLELQRSALLNNIAVRIALARYAATDAELRLEIA
                     RQIPELALKPGYAWDQGDHRWSLGIGYALPLSNRNDGPIAEALARRTAEAARFDALQA
                     LAIAELDAARVLAETSKAQLLEARQQWQASRSHADLVTRRIERGDTDRLEDIAARHAE
                     LDARRALLDAQSALSNAQAGIEDVVQRPLPPAALTLATTDRTATAQSPSRLSDTPQPP
                     DRTR"
     gene            9915..11081
                     /locus_tag="PKV56_05620"
     CDS             9915..11081
                     /locus_tag="PKV56_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67326.1"
                     /translation="MTHRNLVRLLVAQTVLMVLLGWAAVYLGRDEFRLAVGREEEDLP
                     APSLLADEEAGRLPEVRLNSTSQRQAGVELQLPTATSLNGGASGADLAVTVVDPQPLA
                     EWRGRLQTALQELAAARATANASRLEQSRVQALFDDNRSASQRALETANALAQTDAAR
                     ERSAQSQVIALRDSARATWGPIVAGWLEAADGTALQRVLSGREALLRAALRADDTART
                     PARLNLTPPGRSEPVTARGLGTVAPGAAEATAGGRHLLFLAPGAGLATGMRLSARSPG
                     SGDEASREGGLVPASAVIWHAGQAWIYVREAQGGAGEADEAARNGAEPAAAASSPSTV
                     RIDSFQRRGLPNAVRLGDQWFVPGLETDDPVVVRGAQLLLSEELKSQLKNENDD"
     gene            11089..14280
                     /locus_tag="PKV56_05625"
     CDS             11089..14280
                     /locus_tag="PKV56_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX67327.1"
                     /translation="MLAAIVRFSVRHAGLVLGLAVALTGFGVLRMTGASLDVFPEFAP
                     AQVVIQTEAPGLHAAAVEVQVTRPVEQAIAGLAGMTSLRSQSIPGLSVVTALFSESSD
                     LHQNRQGVAERLAQAASSLPQGVVPNITPLTSSASTVLGIGLTSTKLDPIALRALVED
                     SLRPHLMAVPGIADVNVFGGALRQWQVQVDTQRLAQTGLSLTDLATALRSATGVRAAG
                     FIEADNQRIALTMEPPGAELGTPAAAVIDSLSLTVVGQHGGRAVLLRDVAVVAEGGAP
                     AFNAAQIDGQPGVFLMVQGQLGANTRDTTRALDSAIAHIQPVLERAGAKVHLDLFRPA
                     SFIETAVGNVRSDVLIGATLVVAVLFLFLFNLRTAVVSAVAIPLSLIAAVLTLQSFGI
                     GLNIMVIGGLAIALGEVVDDAIIDCENIFRRLRENRALAEPRAAAKVVFDASMEVRSS
                     VVYATFIVALVFTPLLTLSGVAGKLFAPLGLAYIAAIIASLVVALTVTPAMSLLLLRH
                     APLPAADPPLVAWMKPRYRRLLLAIERRSTAVLSAAALCMAAGLGVLPLLGGEFIPPL
                     KEGHYIVHVSAVPGTSLVESLRFGKQVSDTLLAIPGVKSVAQWVGRSQNGADTFGPHY
                     SEMEVEIGQVAGAEQRRILMDIRRALSGDDGDEDRSDEEAAGSTPFPGMVFGVNTFLT
                     ERIGETVSGYPADVVVTLYGPDLDALDRDGKAVVAMLSQMAGATDVQLLAPPGTPELS
                     VRLRPEQLALHAMNAGEVMDSVRIAFDGLVVGQQAGAVTPTPVALVLAPSARQDVSQV
                     ERLPLRTPSGRLIELRDVADVALVEGRYKVLREGGQRVQTVIANLERGHDPEAFVAQL
                     RTRLAHHAKGNDKSGGFAPGNHAEVTGAAEAQAQARSDLLIASALATLGVGSLLLLAF
                     GSLRHTLLTSVNLPFALIGGVVAVLATGGWLSLGSVVGFVTLFGITLRNSIMLVSHYQ
                     HLVEDEGRAWNLETALLGAEERLPSILMTALVTGLGLLPLALGSAEPGREIEGPMAII
                     IVGGLMSSTVLNLLVLPTVMRRYGRFGARELPS"
     gene            complement(14353..14429)
                     /locus_tag="PKV56_05630"
     tRNA            complement(14353..14429)
                     /locus_tag="PKV56_05630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14393..14395),aa:Met,seq:cat)
     gene            complement(14498..14893)
                     /gene="rplQ"
                     /locus_tag="PKV56_05635"
     CDS             complement(14498..14893)
                     /gene="rplQ"
                     /locus_tag="PKV56_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOX67328.1"
                     /translation="MRHRHGLRKLNRTSEHRLAMLRNMCNSLLQHEAIKTTVPKAKEL
                     RRVVEPLITLAKEPTLANRRLAFDRTRDRDIVTKLFNELGPRYKARPGGYTRILKMGF
                     RVGDNAPMAFVELVDRPDVAAAEGGDKAE"
     gene            complement(15032..16018)
                     /gene="rpoA"
                     /locus_tag="PKV56_05640"
     CDS             complement(15032..16018)
                     /gene="rpoA"
                     /locus_tag="PKV56_05640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOX67329.1"
                     /translation="MQTNLLKPKAINVEPLGVNRAKVTLEPFERGYGHTLGNALRRVL
                     LSSMVGYAPTEVTIAGVLHEYSTVDGVQEDVVHIMLNLKGVVFRLHNRDEVTLVLRKD
                     GEGPVTAGDIQTPHDVEIINPNHVIANLSQGGKLDMQIKVEKGRGYVPGNMRRFGDEP
                     TKSIGRIVLDASFSPVARVSYTVESARVEQRTDLDKLVMEINTNGAISPEEAIRASAK
                     ILVEQLAVFAQLENSEIAAFNEPQSRNTQFDPILLRPVDELELTVRSANCLKAENIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDKR"
     gene            complement(16169..16792)
                     /gene="rpsD"
                     /locus_tag="PKV56_05645"
     CDS             complement(16169..16792)
                     /gene="rpsD"
                     /locus_tag="PKV56_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOX67330.1"
                     /translation="MARYLGPKAKLSRREGTDLFLKSARRPIGDKAKFDSKPGQHGRT
                     SGSRTSDFGLQLREKQKVKRMYGVLERQFRRYFAEAERRKGNTGANLLMLLESRLDNV
                     VYRMGFGSTRAEARQLVSHKSITVNGGVVNIASYMVKVGDAVSVREKAKKQLRIADSL
                     KLAESMGLPAWIQVDAAKMEGVFKKTPDRDEFGADIKEALIVELYSR"
     gene            complement(16983..17384)
                     /gene="rpsK"
                     /locus_tag="PKV56_05650"
     CDS             complement(16983..17384)
                     /gene="rpsK"
                     /locus_tag="PKV56_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOX67331.1"
                     /translation="MAKAPNTAAQRVRKKARKNVADGIAHVHASFNNTIITITDRQGG
                     ALSWASSGGQGFKGSRKSTPFAAQVAAEVAGRAAQEQGIKNLDVRIKGPGPGRESSVR
                     ALASLGIRINLISDVTPVPHNGCRPQKRRRI"
     gene            complement(17441..17806)
                     /gene="rpsM"
                     /locus_tag="PKV56_05655"
     CDS             complement(17441..17806)
                     /gene="rpsM"
                     /locus_tag="PKV56_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOX67332.1"
                     /translation="MARIAGINIPPHKHAEIGLTSIYGIGRTTAQKICVNSGVPFDRK
                     IKDLTDTDLERLRDEIGRLTIEGDLRREMSINIKRLMDLGCYRGFRHRRGLPVRGQRT
                     KTNARTRKGPRKSGALVKK"
     gene            complement(17845..17958)
                     /gene="rpmJ"
                     /locus_tag="PKV56_05660"
     CDS             complement(17845..17958)
                     /gene="rpmJ"
                     /locus_tag="PKV56_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOX67333.1"
                     /translation="MKVAASVKKMCRNCKVIRRKGVVRVICTDPRHKQRQG"
     gene            complement(18019..18237)
                     /gene="infA"
                     /locus_tag="PKV56_05665"
     CDS             complement(18019..18237)
                     /gene="infA"
                     /locus_tag="PKV56_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOX67334.1"
                     /translation="MAKDDVIQMQGEILENLPNATFRVKLENGHIVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            complement(18290..19600)
                     /gene="secY"
                     /locus_tag="PKV56_05670"
     CDS             complement(18290..19600)
                     /gene="secY"
                     /locus_tag="PKV56_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOX67335.1"
                     /translation="MATNANQLAKSGKFGDLRRRLTFLLLALVVYRMGAHIPVPGIDP
                     NQLAELFKGQAGGILSLFNMFSGGALSRFTVFALGIMPYISASIIMQLMTYVVPTLEA
                     LKKEGEAGRRKITQYTRYGTLGLAIFQSLGIALALEGSQGLVISPGFGFRLTAVVSLV
                     AGTMFLMWLGEQITERGLGNGISILIFAGIAAGLPSAIGGLFELVRTGSMSYVACLFI
                     LVVVGFVTFAVVYVERGQRKILVNYAKRQVGNKVYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILLPATVVGWFATGDGLRWLKDLAGLLSPGQPIYVLLYATAIVFFCFFYTALVFNS
                     RETADNLKKSGAFIPGIRPGDQTARHIDRILSRLTLAGAVYITLVCLLPEFLVLKYNV
                     PFYFGGTSLLIIVVVTMDFWAQVQSYVQSQQYESLLKKANFKPS"
     gene            complement(19641..20072)
                     /gene="rplO"
                     /locus_tag="PKV56_05675"
     CDS             complement(19641..20072)
                     /gene="rplO"
                     /locus_tag="PKV56_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOX67336.1"
                     /translation="MELNTIKPAEGSKRQRRRVGRGIGSGLGKTAGRGHKGQKSRAGG
                     YHKVGFEGGQMPLQRRLPKRGFKSASLKYNAEVTLTDLQRLGVAEVDLLTLKQAGLVG
                     EIAKVVKVIKTGELTLAVTLKGVGATAGAKAAIEAAGGSVA"
     gene            complement(20095..20277)
                     /gene="rpmD"
                     /locus_tag="PKV56_05680"
     CDS             complement(20095..20277)
                     /gene="rpmD"
                     /locus_tag="PKV56_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOX67337.1"
                     /translation="MADKPTVKIKLVRSIAGTRESHRATVRGLGLRKLNSESELEDTP
                     AVRGMINKISYLLKVL"
     gene            complement(20297..20815)
                     /gene="rpsE"
                     /locus_tag="PKV56_05685"
     CDS             complement(20297..20815)
                     /gene="rpsE"
                     /locus_tag="PKV56_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOX67338.1"
                     /translation="MAKFQPRAQTEGNDDGLKEKMIAVNRVTKVVKGGRTLSFAALTV
                     VGDGDGRIGMGKGKAKEVPVAVQKAMEAARRNMVKVSLKNGSIHHKVVGEHGASHVLM
                     APAPAGTGIIAGGPMRAVFEVMGVTDIVCKSLGSSNPYNLVRATLDALKHTSTPAEVA
                     AKRGLTVEAIFN"
     gene            complement(20831..21196)
                     /gene="rplR"
                     /locus_tag="PKV56_05690"
     CDS             complement(20831..21196)
                     /gene="rplR"
                     /locus_tag="PKV56_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOX67339.1"
                     /translation="MLNKKDQRTRRSRQTRVRIARQRVVRLTVFRTNLHIYASVISDD
                     GNRVLASASTVEADVRKELGAAGKGGNVGAAALIGKRIAEKARAAGVEKVAFDRAGFA
                     YHGRVKALAEAAREAGLQF"
     gene            complement(21209..21742)
                     /gene="rplF"
                     /locus_tag="PKV56_05695"
     CDS             complement(21209..21742)
                     /gene="rplF"
                     /locus_tag="PKV56_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOX67340.1"
                     /translation="MSRVGKMPVSVPQGLDVAITAEQITVKGPLGTLVRPANPLVNIS
                     NTAGKLEFIPADATTAADAMSGTMRALVANMVNGVSKGFEKRLSLVGVGFRAQAAGSK
                     LNLQIGFSHPVGKDMPDGVKVECPTQTEIVIKGSDRQVVGQIAAEVRAIRPPEPYKGK
                     GIRYVGEKVTIKETKKK"
     gene            complement(21758..22153)
                     /gene="rpsH"
                     /locus_tag="PKV56_05700"
     CDS             complement(21758..22153)
                     /gene="rpsH"
                     /locus_tag="PKV56_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOX67341.1"
                     /translation="MSMSDPIADMLTRIRNAQMVEKPSVSMPSSKLKVAIAQVLKDEG
                     YVDSFAVVGEATKPQLQIALKYYAGKPVIEHIERVSRPGLRVYKGRHDIPNVKNGLGV
                     AIVTTPRGVMTDRKARQVGIGGEVLCYVA"
     gene            complement(22181..22486)
                     /gene="rpsN"
                     /locus_tag="PKV56_05705"
     CDS             complement(22181..22486)
                     /gene="rpsN"
                     /locus_tag="PKV56_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOX67342.1"
                     /translation="MAKLSLIQRELKREQLAAKYAKKYAEYKGIANDAKKSDEERYAA
                     RLELQKLPRNAYPTRQRNRCALTGRPRGTFRKFGLGRNKIRELAFKGDIPGVIKASW"
     gene            complement(22495..23049)
                     /gene="rplE"
                     /locus_tag="PKV56_05710"
     CDS             complement(22495..23049)
                     /gene="rplE"
                     /locus_tag="PKV56_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOX67343.1"
                     /translation="MAQQQPARLQAIYRDTIAPGLIAKFGYKSAMEVPRITKITLNMG
                     VSEAVSDKKVMEHAVSDMTKIAGQKPVVTKSKKAIAGFKIRDGVPIGCMVTLRGVQMY
                     EFLDRFVTIALPRVRDFRGISGRSFDGRGNYNIGVKEQIIFPEVEYDKVDALRGLNIS
                     ITTTAKNDDECKALLAAFKFPFKN"
     gene            complement(23059..23376)
                     /gene="rplX"
                     /locus_tag="PKV56_05715"
     CDS             complement(23059..23376)
                     /gene="rplX"
                     /locus_tag="PKV56_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOX67344.1"
                     /translation="MNKIRKGDQVIVLTGRDKGKRGVVAARIDEDRITIDGVNIVKKH
                     VKPNPMKGTTGGVVEKTMPIHQSNVAIYNSASGKADRVGIKLLADGKKVRVFKSSGEE
                     IKV"
     gene            complement(23397..23765)
                     /gene="rplN"
                     /locus_tag="PKV56_05720"
     CDS             complement(23397..23765)
                     /gene="rplN"
                     /locus_tag="PKV56_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOX67345.1"
                     /translation="MIQMQSRLDVADNTGAKSVMCIKVLGGSRRRYAGIGDVIKVSIK
                     EAAPRGRVKKGEVYSAVVVRTAKGVRRQDGSLVKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(24016..24297)
                     /gene="rpsQ"
                     /locus_tag="PKV56_05725"
     CDS             complement(24016..24297)
                     /gene="rpsQ"
                     /locus_tag="PKV56_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOX67346.1"
                     /translation="MTEAQPAVKEKTVRTLVGKVVSDKRSKTITVLIERRTKHELYGK
                     IVAKTSKYHAHDENNEYKNGDVVEISESRPISKTKAWVVTKLLQKAREV"
     gene            complement(24294..24503)
                     /gene="rpmC"
                     /locus_tag="PKV56_05730"
     CDS             complement(24294..24503)
                     /gene="rpmC"
                     /locus_tag="PKV56_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOX67347.1"
                     /translation="MKASELRAKDIAGLQKEVSDLLKAHFGLRMQKATQQLANHTQLG
                     NTRRDIARAKTVIAQKQREVKGAAK"
     gene            complement(24524..24940)
                     /gene="rplP"
                     /locus_tag="PKV56_05735"
     CDS             complement(24524..24940)
                     /gene="rplP"
                     /locus_tag="PKV56_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOX67348.1"
                     /translation="MLQPARRKYRKEQKGRNTGVATRGAKVSFGEYGLKATERGRLTA
                     RQIEAARRAISRHVKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEINGVPEALAREAFTLAAAKLPLSCTFVARQVGA"
     gene            complement(24943..25824)
                     /gene="rpsC"
                     /locus_tag="PKV56_05740"
     CDS             complement(24943..25824)
                     /gene="rpsC"
                     /locus_tag="PKV56_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOX67349.1"
                     /translation="MGQKIHPIGFRLPVTRNWASRWYANNQNFATMLAEDLKVREFLK
                     ARLKNAAVSRVLIERPAKNARITIYSARPGVVIGKKGEDIESLKAELTRRLGVPVAVN
                     IEEVRKPEIDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKLMSSGRLNGI
                     EIARCEWYREGRVPLHTLKADIDYGFSEAKTTYGIIGVKCWVYRGDRLGHGEAPGVVN
                     KPEGEDDRRPRRNARPGAPPSRGRPGAPGADRGPRAAGPADLAAKPSEAGDPSKAPTV
                     KRVRKAVPPTQGEAKGE"
     gene            complement(25835..26164)
                     /gene="rplV"
                     /locus_tag="PKV56_05745"
     CDS             complement(25835..26164)
                     /gene="rplV"
                     /locus_tag="PKV56_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOX67350.1"
                     /translation="METRSIVRGVRLSADKGRLVADLVRGRPVDQALNILTFTPKKAA
                     GIIKKALESAIANAEHNDGADIDELKVKTIYVEQGAILKRFSARAKGRGNSICKPTAH
                     IYITVGN"
     gene            complement(26174..26452)
                     /gene="rpsS"
                     /locus_tag="PKV56_05750"
     CDS             complement(26174..26452)
                     /gene="rpsS"
                     /locus_tag="PKV56_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOX67351.1"
                     /translation="MTRSLKKGPFVDHHLMAKVDKAVAIKDKKPIKTWSRRSTILPDF
                     IGLTIAVHNGKQHIPVYVTDQMVGHKLGEFALTRTFKGHPADKKAARK"
     gene            complement(26465..27289)
                     /gene="rplB"
                     /locus_tag="PKV56_05755"
     CDS             complement(26465..27289)
                     /gene="rplB"
                     /locus_tag="PKV56_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOX67352.1"
                     /translation="MAVVKVKPTSPGRRAVVKVVHSHLHKGKPEASLLEPQFQHAGRN
                     NNGHITIRHKGGGHKHHYRVVDFVRNKDGIPAKVERIEYDPNRTAHIALVCYADGERR
                     YVIAPRGLEIGQTLLSGAEAPIKAGNTLPIRNIPVGSVIHCIEMLPGKGAQIARSAGT
                     SCTLLAREGTYAQVRMRSGEVRRIHIDCRATIGEVSNEEHSLRQYGKAGAIRWKGIRP
                     TVRGVAMNPVDHPHGGGEGRTGEGQVPVSPWNTMTKGYRTRNNKRTQTFIVSRRKK"
     gene            complement(27289..27606)
                     /gene="rplW"
                     /locus_tag="PKV56_05760"
     CDS             complement(27289..27606)
                     /gene="rplW"
                     /locus_tag="PKV56_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOX67353.1"
                     /translation="MSAPVKHAENRLAQILIAPIVSEKATMASEKHNQVLFKVLRDAT
                     KPEIKAAVELMFKVEVESVQTVLQKGKVKKFGRSIGRRDHVKKAYVSLKAGQELNISG
                     EAA"
     gene            complement(27603..28223)
                     /gene="rplD"
                     /locus_tag="PKV56_05765"
     CDS             complement(27603..28223)
                     /gene="rplD"
                     /locus_tag="PKV56_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOX67354.1"
                     /translation="MQLELLNAQGQATSKVDAPDTLFARDYNEALVHQIVVAFQANAR
                     QGTRAQMDRAEVKHSTKKPWRQKGTGRARAGMTSSPLWRGGGRIFPNSPDENFTQKVN
                     KKMYRAGMAAIFSQLAREGRLAVIDSMEVDSPKTKLFAAKLKAMGLDSVLVIADQVDD
                     NLLLASRNLRNVLVVEPRHADPLSLVHYKKVLVTKAAIEQLKEMLA"
     gene            complement(28223..28894)
                     /gene="rplC"
                     /locus_tag="PKV56_05770"
     CDS             complement(28223..28894)
                     /gene="rplC"
                     /locus_tag="PKV56_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOX67355.1"
                     /translation="MSLSNRLGLLGRKVGMMRIFTDDGDAIPVTVLDVSNNRVTQVKT
                     DETDGYTAIQVAYGARKASRVNKPEAGHLAKAGVEAGQILKEFRVAADVAAGFKPGAQ
                     IPVTTFAVGQLVDVQGTSIGKGFTGTIKRHNFSSQRASHGNSRSHNVPGSISMAQDPG
                     RVFPGKKMSGHRGDVTKTTQNLDIIRIDEARQLLLVRGAVPGSKNGHIVVSPAVKVKV
                     KKGAN"
     gene            complement(28982..29293)
                     /gene="rpsJ"
                     /locus_tag="PKV56_05775"
     CDS             complement(28982..29293)
                     /gene="rpsJ"
                     /locus_tag="PKV56_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOX67356.1"
                     /translation="MQKQKIRIRLKAFDYKLIDQSALEIVDTAKRTGAIVKGPVPLPT
                     RMQRFDILRSPHVNKTSRDQFEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            complement(29393..>29469)
                     /locus_tag="PKV56_05780"
     CDS             complement(29393..>29469)
                     /locus_tag="PKV56_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOX67357.1"
                     /translation="EGLRFAIREGGRTVGAGVVAKIIE"
BASE COUNT         5216 a         9504 c         9215 g         5534 t
ORIGIN      
        1 gcgcgagaaa accaccgccg agaacatcga tgtcaggatg cccaggcagt ggacaaccgc
       61 gaagccgcgc accgggccgg atccgaacgc cagcagcgag agcccggcaa tcagcgtcgt
      121 gacattggag tccaggatgg tcgtgaacgc gcgctcgtat ccgttgtgga tcgccgactg
      181 gggtgatgcg ccactgcgca gttcctcgcg cacacgctcg ttgatcagca cgttggcgtc
      241 gattgccatg ccgagcgtca gcgcgatggc cgccatgccc ggcagcgtca ggctggcctg
      301 caacatcgac agcaccgaca ccagcagcag caggttgaag gccagggcca gtgtcgagat
      361 gatgccgaaa agcatgtaat agaggcacat gaagaccgcg atcgccgcga agccatacag
      421 cacgctgttg aaccccttgg agatgttgtc ggcgccgagg ctcgggccga tcatgcgctc
      481 ctcgatgatg tccatcggcg cggccagcga gccggagcgc agcagcagcg aggtatcggc
      541 cgcttcggtc gtcgtcatgc gacccgagat ctgcacccga cccccgccga tctcggcgcg
      601 gatcaccggt gcggtcacga tctcgccctt gcctttctcg aacagcagga tggccatgcg
      661 cttgccgatg ttgtcgcgcg tcacatcctt gaagatgcgt gatcccttgg catcgagcgt
      721 caggttcacc gtgggttcct gcgtttgccc atcgaagccc gcctgggcat cggtcaggtt
      781 ctctcccgtg aggatgacct gccgtttgag gatcagcgcc gcgccgttgc gctcggtgta
      841 acgctcagtg ccgaacggca cagggccggt tccacccgcc gcggccgcgg cttcggtgct
      901 ttcgtcgacg agacgcactt ccagcgtcgc cgtgcggccg atgatgtcct tggccttcgc
      961 ggtgtcctgc acgcccggga gttgcacgac gacccggtcg atgccctgct gctggatcac
     1021 cggttcggac acgccaagct cgttgacccg gttgtgaagc gtggtgatgt tctgcttgac
     1081 cgcggactcc tgcacacggc gcgacgcctc gggcttcagt gcacctgcca gcttgatatc
     1141 ggtgccatcg gggacttcgg tccactgcag atcgcgcaac tcgtcttcga gcagttggcg
     1201 cgccgcggta agcgtttcgc gatcgcggaa gcgaatcacg accgcattgc cctcgcgcac
     1261 gatgcccgca tggcgcaggt tcttgtctcg cagcatcagg cgcacatcgc cggtcagcga
     1321 ctcagctttc ttggtcagcg ccgatttcat gtcgacctgc atcaggaagt gcaccccgcc
     1381 tcgcagatcc agacccagcg acatgggcag cgcgtgcagc gatgcgagcc agtgcggcga
     1441 acgtgacagc aggttcaacg cgacgatgta gctgggatcg gcaggatcgg gattcaatgc
     1501 cttgacgatg gcatccttag ccttgatctg cgcatcggta tcggcgaagc gcgccttcac
     1561 cgagttgccc tcgaactgca cgaagtcggc ctgcaggccg gacttcgtca gcgcttcctc
     1621 gacgcgtgtg gccacgctgc tgtcgatctt caaagtggca cggccactgc tgacctgaac
     1681 tgcaggcgcc tcgccaaaca gattgggcag cgtgtagatc aggccgacca ccaaggcggc
     1741 cagcaggatc gcgtacttcc agaggggata acggttcatg acgtctttct ggcggtcgat
     1801 cggaaggcgg tcagcccggt gagcgccgag cgacggccgg gattgcgtcg gccgcgcggt
     1861 ggactgacgc agtccctact tgaaggttcc cttggggaga acctgaacga ctgacgagcg
     1921 ctgcacctgg atctcgaccc cattggccac ttcgacaccg aggtacgtct cgcccatctt
     1981 ggcaaccttg ccaagcacac cgccgccgat gactacctcg tcgcccttgg cgagcgcgtc
     2041 gatcatggcc ttgtgctcct tctgccgctt catctgtggg cggatcatca cgaagtacag
     2101 caccacgaac atcaggatca ggggcagcat ggaaccgatg ctgccgagca tgtcggggct
     2161 gctggtggct gcgggtgcgc cctgtgcgaa ggcttgcgaa atgaacacgt gaattcctct
     2221 tgaggggccg cgtgaaacgc cacgggcaaa accccgggat tgtacggtgg tgactcgtgc
     2281 gagcgcccct gctctgagcc acggcgcccc atgacacgga tggcggcaca ggttgcaaat
     2341 cgcttacacc gcccgacgcg cgcagcagcg aagctcaagc ccacggcagc aacagggtct
     2401 cggatcgggg ccgctggatg gcatatgccg gcaccctcat gcaagcacat ctcgatctga
     2461 acacgaaagg ccgaactatg cgcgccgcgc tcggcgccat ggccctcgct gccgtgctgt
     2521 cgctggccgg atgtggtgga ggtgggggcg gcagcgacgc cacggcatcg aatccggaca
     2581 gctcgggcac gacgccaccc ccgacctccg ccaccggggg cagcgccccg gcgcgtccgg
     2641 cgccggaaga caggccgcag tcgacagacg cggctcgact gctgacacag gcgacattcg
     2701 ggatcacatc ggtgggcgag atcgaggccg tgcgcaccga cggctacgag cgctggctct
     2761 ggacgcagtt caataccccg gccgccttgc acacccgata cctcgacgcg cagaagggcc
     2821 gcgaaaagaa ccgcaaggcc accgaggaga tgagctacga ggcggtgtgg cagcagtggc
     2881 tcaccggcgc cgaccagctg cgcgcgcgca ccgcgttcgc tctcggccag atcctggtga
     2941 tctcgaacat cgcgcccgac atccggccct acgcgatgtc ctcgtacatg gacctgctga
     3001 accgcaacgc cttcggcaac taccgcaccc tgctcaggga ggtcacgctg cacccggcga
     3061 tgggctacta cctgaacatg atcgagagcg aaaaggatga cgaggtgcgc ggcatccacc
     3121 cgaacgaaaa ctatgcgcgc gaggttctgc agctcttctc gatcggactg gtccagctca
     3181 acatcgacgg cacaccgaaa gtcggtaccg atggcaagcc gctggcgacc tacggtgagg
     3241 cgcaggtgaa ggggtttgcc aaggcgttct ccggctggac cttcgcgtcg cagaacccgt
     3301 cgcagcccga cagcttccac gacgccaatg aggaactcga cgcgaactgg acgacgccga
     3361 tgcgggcttt cgccagcgca cactctcccg agcccaaggc gctgctgaac ggcgtcacgt
     3421 tacccgcggg tcagacgccc gagaaggacc ttgaagatgc aatcgatgtg atcttcaacc
     3481 acccgaacgt cgggcccttc atcgggcggc agctgatcca gcgattggtc accagcaacc
     3541 cgagtccggc ctacatcgcc cgtgtggcgc gcacattcaa cgacaacgga tccggggtgc
     3601 gcggggactt gaaggcggtg gtgcgagcca tcctgctcga ccccgaggcg cgcgatgtct
     3661 ccctggctgc aggcgaccgc tacggcaagc agcgtgagcc ggtggctcgc ttcgccagct
     3721 tcctgcgtgc cctcggcgca aagagccgca acggcatcaa cagcatccac tacctcgaca
     3781 gcgcggacaa cggcctcggg cagagcccgc tgctggcgcc atcggtcttc aacttctact
     3841 cgcccaactt ccgtcagccc ggtgcgatcg cgagggcggg gctgttttcg ccggagttcc
     3901 agatcacgac cgaaacctcg gtggtcggat cactgaattt tttcgcagat ctattcaaca
     3961 gcgagggcta cggcgacggc aatagccggc tggtgctcga tctgaagccg ttggctgcgc
     4021 tggctgccac gccctctgcg ctgatcgacc ggctcgacgc actgttcttt gcacaacaga
     4081 tgcgccagag cacgcgggct cggctgaccc gtctgctgga gtcgctgccc gggacctcgg
     4141 agtacgagct gtcgcagcgg gtcaaggctg cactgatcgt cgtctcgctg tcgcccgact
     4201 tcgtgatcca gaaatgaagg ccaacgccat gccctcctct gcactgcgcc ggcgacagtt
     4261 cctgaagctt gccggtgccg cgaccgccag cggcctggga ctgggcaccc tggcgcacct
     4321 gtcggcttct gcgcaaagcg tcggcgatta caaggcgctg gtgtgcctgt tcatgtacgg
     4381 cggcaacgac ggcaacaacc tgctggtgcc gctcgagctc ccggcctacg acggctaccg
     4441 gcgcgcgcgc accaacctcg cgctggaccg cgacgccctg ctgccgatcg caccgaccaa
     4501 caccagcggc gcgcgctatg gcctgcaccc ggcgatggcc ggattgcagg gcctgttcac
     4561 ccagggcaag gccgcgatgc tggccaacat cgggtcgctg gcggtgccca ccagcaaggc
     4621 gcaatgggag gcgaggtcgg tgccactgcc cgacaacctg ttctcgcatt cggaccagca
     4681 ggcgcagtgg cagagcgcga tcgtcgatgc tcccccgcgc agtggctggg gaggacggat
     4741 gatcgagcga ttgatagcag ccgagatcgc gaatcgcggc tacggcctgg tgtcgatggc
     4801 cggtggcaac ctctgggaaa acggcgaccg ttcgctggcg gcctacaagg tctcatcggc
     4861 gggccgtttc ggtttcgact tctatgacgc cagcggcggc gacccgttgt ctgccgcgat
     4921 cagcgacacg ctttccgaac cccgcagcca cctgctcgaa caggcctgga ccgatgtggt
     4981 cagccgctcg atagacaacc agcgcatcct gacgcaggcc ctgtcgggca gcgagctcac
     5041 gacggtcttc ccgggtaccg ggctcggcca gcaactgcag atgatcgccc ggctgatctc
     5101 ggcgcgcagc cggctcggcc tgtcgcgaca gtgcttcttc tgctcgattg gcggcttcga
     5161 cacccatggc gacgaccagc tgcaacggca gaccgaactc ctgggcgaga tctcgacggc
     5221 agtgactgcc ttctacgagg cgacggtggc actcggggtg cagaacgacg tcgcgctgtt
     5281 ctcggcgtcg gacttcggtc gcaccttcca gagcaacggc cagggctcgg accacgcctg
     5341 gggcagccac catttcgtca tcggcggcgc ggtgaagggc gggcagatcg tcggccgcta
     5401 tcccgaactg atcgtcaacg gaccggacga cgccggcgac ggcaagtgga tcccgaccac
     5461 gtcggtcgat cagctcggcg ccacactggg ccgctggttc ggcgccgcgg cgtcgctgaa
     5521 cgaggtgttt ccgaggctgt cgaacttcga cgccgacctc ggattcatga cctgattcac
     5581 atccggcgga tcgcctcgcc cggggtcaat cgctcgccag gggaccgaac gggcagcgtc
     5641 cgaacgcggc actcagccgg cgcagccgcg gtgcgggctg cgcgccgacc atcggccagt
     5701 cgaagcgcca ggtccagttg ccggtcgccg tgccgggcgt gttcatgcgg tgcgaggcat
     5761 cgaggttcaa cacgtcctgc atcggaaaca ccgacaggtt cgccaccgac gcgctggccg
     5821 cacggatcgc ggcccaggcc acgcgcgtgt ctgcttcggc ggcgtcgcag tcatcgagcc
     5881 cgaagaccag cccggcatag gcccgctcgg ccgcgctggc cgactgccac caaccgcgcg
     5941 tggtgtcgtt gtcgtgcgtg ccggtgtagg ccacggtatt cgcggcgtag cggtgcggca
     6001 ggtaatcgtt ttcggcgtcg ccgccgaacg cgaagtgcag gatgcgcatg ccgggcagac
     6061 ccaggccgtc gcgcaaggac tcgacatcgg gtgtgatcac gcccaggtcc tcggcgacga
     6121 tgggcagcac accgagcgcc tgctcgatcg cgtcgaacag aggtcggcct gggccgggca
     6181 cccagtggcc ctcgatcgcg gtcggacagt cggcaggtat ctcccagtac gctgcgaagc
     6241 cgcggaagtg atcgatgcgg aacacatccg cctgatcgag cgcgcgtcgg acgcgagcaa
     6301 tccaccacgc gtatccctcg tcggccattc ggtcccaccg atacagcggg ttgccccatc
     6361 gctgtccggt cgcactgaag aaatcgggag gcacgccggc caccaccgtc ggctggaaag
     6421 cgtcgtccag ccagtacagg tcgggcctcg cccagcagtc ggcgctgtgc tgcgcgatga
     6481 agatcggcag gtcgcccatc agcgccacac cgcgtcgacg ggcgtgcgcc cgcaccgcct
     6541 gccactgcgt gtcgaaacac cactgcacga agcgccagaa gaacagctcg tcggtctgcg
     6601 ctgcgctggc tgccgccagt gcgtcggggt cgcgacgggc cagcggcgag tcccagtccc
     6661 accacgcccg ccagccgcca tcgcgggatt gggactgttc aagcgccatg aagagggcgt
     6721 agtcgtcgag ccagtgcgac tgcgcgcggg accagtcaga gaaagcctga ctgtcggcag
     6781 tgcaggcccg cgatgcaaag cctgcggcag cctcgcgcag cctcgcatcg cgccacggaa
     6841 cgaccttggc gtaatcgacg cgcgacgcgt cgaagtcggc ttcgaccacc gccgcggcca
     6901 gcgcgtcggc ggccagccag ccacgctcga ccagaggctc gagagcgacc agcagcggac
     6961 tgccagcgaa cgcggacacg ctctggtagg gagagtcccc aggtccgatc ggcgtcagtg
     7021 gaagcacctg ccacaagcgt tggccggcgt cgtgcagcca gtcgatgtag tgatgcgccg
     7081 ccggcccgag atcgccgctg ccatgcgggc ccggcagcga ggtgacgtgc aacagcacgc
     7141 cagaggctcg ttccgtcaga ttcattccac gccctccccg gcctgcttca tctccagtgc
     7201 cagcgcagcg ccagccagag ccgcctgcgg atcgaggatc agtgcggtcg ggatcgcctg
     7261 cagatagccg cgatagcggc ccttggcctc gaagcgctcg cggaaggccg aggcaaagaa
     7321 cagctcggcg atacgcggca cgatgccccc cccgatgtac aggccgccgc gcgcaccgaa
     7381 cgtcaatgcg acattgccgg cgaacgaacc gagcatcgcg cagaacaccg acagtgtctg
     7441 cgcgcagccc gcatcaatgc caccaatgcc acgctgaatg atcacttcgg tgacaagcga
     7501 atcggccgga cgccccgaca cggcagccac tgcgcgatgc agcagcggca ggccgatgcc
     7561 ggacagcagc cgctcggccg acacgtgctc cagctcgcgg cgagcaaagc tcagcaccgc
     7621 cgactcgaaa tcgtcggcac ccgacaacgt gacatgcccg ccttcgcagg gaatgggctg
     7681 ccagccggcc gctgtctcca gcaacccggc cacgccaagg ccggttcccg gaccgatgac
     7741 cgcgagggtg ccgtgcggct cgggggccgc ggcgtgcgcg cgcaacccgt ccgaagcgag
     7801 tcgcggaagc gccagcgcca gcgcctcgaa atcgttgagc agttgcagat ggtcgagggc
     7861 caggtcgcgc tgcaacgcat tgcgcgagaa ccgccagcgg ctgttcgtga agatcacctc
     7921 atcgccatcg agcggtgtgg ccacggcaaa gcaggcgcag gtcggggcgg cacacaacga
     7981 cgcgaccgat gccaggtaac agcgcgccgc atcgaccaga ttcgcatgct cgtcggcacg
     8041 caggcgctgg acatgctcga ccgcacggcc cggcccgacg accagaccga accgggcgtg
     8101 ggtgccaccg atgtcggcaa ccagccaggg cgccccagcc gggtgcattg ccatcttcga
     8161 agccatgtcg cggatccttc atgaaacgag cgtgctgcat gacggcagag tacagcgcga
     8221 gaagcatgcc agcgctggtg cgaccgcagc atgaagcggg tttcgcccgg tcggccggga
     8281 acgtttcccg actacgatcg gagcccgccc gggcccggcc agacgccacc gatcccagtg
     8341 ggactgcctg cccgattgca cctgcccgcg cctgcctcga tgaccgcact gactaacgcc
     8401 ctgcgtcgcg ctgcccatca ccgcgtggtc gcactgggta tctgcactgt catgctgacg
     8461 ggttgtgcac tccagaggca cgagcctcgc gccctcgatc cggcagtttc gcagtcagcg
     8521 cagctttctc gccgggccga cgatgcaaca tggcgcgagg cgatgtcgcg actcggggtg
     8581 gacaccacga tctggcctct gccggcgtgg gacgcgcccg agctgagtgc gttcgccctg
     8641 gcatcgcacc ccgaactggc agtcgcgcgc accgagatcg ccgtggcgca gtccgcaggc
     8701 cagacggctc ggcaacgtgc caacccatcg atcgacctga gcctggagca ccacagcgcg
     8761 cccgggcaga gcgactcgcc ctggaccatc ggtatcgcac tcgactcgct ggtcggcgag
     8821 tggctgctgg gccagagccg ccgcgcggcc ctgaccgaac tggccgatgc ccggcagctc
     8881 gaagcggtcg agcgcgccgc tcaggtcgcc tggcagttgc accgccgcgt gcgcgacagc
     8941 caccgtgcgc ttcacgatgc cgagaagcag tcggccctgg ccgacgagat gctggcgctg
     9001 cagcgcgagg aaagcgtgct gtggcaacga cgcatgacgc tgggcgccgc tggtgctccc
     9061 gaagtgctgc cggcgcagca gcgacaggcg gttgcccgtc agcgggcgca ccaggctcgc
     9121 ctggcggtgg atcaggcgcg tgtcgcactg gcccaggcga tcggtctgcc gctggagcag
     9181 ctgcagcaca tgaccattcg cttcgactcg ctggatcaac ctcagcccga tcagcgggcg
     9241 attaccgagt cggcgacagc ctctgcgccc gtcctggaac cgctggagct gcagcgctct
     9301 gccctgctca acaacattgc cgtgcgaatc gcactggcgc gctatgcagc caccgatgca
     9361 gagctgcggc tggagattgc gcgccagata cctgagctgg cactgaagcc aggctatgcc
     9421 tgggatcagg gcgaccaccg ctggtcgctg ggcataggct acgcgctgcc cctgtcgaat
     9481 cgaaacgacg ggccgatcgc cgaggcgctc gcgcggcgca cggccgaggc tgcacgtttc
     9541 gacgcattgc aggcgctagc gatcgcggag ctcgatgccg cccgagtgct cgccgagacc
     9601 tcgaaagcgc agttgctgga ggcgcgacag cagtggcagg cgtcgcgatc acacgcagac
     9661 ctggtgacac gccggatcga gcggggcgac acggatcgac ttgaagacat cgccgcacgc
     9721 catgccgaac tcgacgcccg ccgtgcgctg ctcgacgctc aatccgcgct gagcaacgcg
     9781 caagccggga tcgaggatgt cgttcagcgg cccctcccgc cagcggccct cacgctcgcg
     9841 accacggacc gcacggccac agcccagtct ccatctcgcc tgtccgacac tccacaaccc
     9901 ccggaccgca cccgatgacc caccgcaacc tcgttcgcct gctcgtcgca cagaccgtgc
     9961 tgatggtgct tctcggctgg gccgccgtct acctgggacg agacgaattc cggctcgcag
    10021 tgggacgcga ggaggaagat ctacccgcgc cctctctgct ggccgacgag gaagccggtc
    10081 gattgcccga ggtgcggctc aacagcactt cgcagcggca ggccggcgtc gagctgcagt
    10141 tgccgacggc gacctcattg aacggcggtg cgtcaggtgc cgacctcgcg gtgaccgtgg
    10201 tcgatccgca gcctctggcc gagtggcgcg ggcgcctgca gacggcgttg caggagctgg
    10261 cggcagcgcg cgccactgcc aacgccagcc ggctcgagca gtctcgggtg caagcgctgt
    10321 tcgacgacaa ccgcagcgca tcgcagcgcg cgctggagac cgcgaacgcg ctggcacaga
    10381 ccgatgcagc gcgggagcgc agtgcgcaat cgcaggtgat cgcactgcgc gacagtgccc
    10441 gtgccacctg gggaccgatc gtcgcaggct ggctcgaggc agccgacgga acggccctgc
    10501 agcgggtgct gtcgggtcgc gaggcgctgt tgcgcgcggc gctgcgcgcc gacgacactg
    10561 cccgcacccc ggcacggctg aacctcacac cgcccggccg cagcgaaccg gtcacggcac
    10621 gcggcttggg cacggtcgca ccgggcgccg ccgaagcaac cgcgggaggg cgacatctgc
    10681 tgtttctcgc tccgggagcg ggcctcgcca ccggcatgag gctgtcggct cgcagcccgg
    10741 gcagcggtga cgaagcctcc cgcgaaggcg ggctggtgcc cgccagcgca gtgatctggc
    10801 acgcaggaca ggcctggatc tatgtgcgcg aggcgcaagg tggggcgggc gaggccgatg
    10861 aggctgcccg aaacggcgcc gagcccgcag cagctgcctc gagcccctcc acggtacgca
    10921 tcgacagctt ccagcgtcgc ggactgccga atgcggtgcg cctgggtgac cagtggttcg
    10981 tgcccggact ggagaccgac gacccggtgg tggtgcgagg cgcccaactg ctgctgtcgg
    11041 aagagctgaa gtcccagctc aagaacgaga acgacgactg accccgggat gctcgctgcc
    11101 atcgttcgct tttcggtgcg tcacgccggc ctggttctcg ggctggcggt ggcgctgaca
    11161 gggttcggcg tgctacgcat gacgggggcg agcctcgacg tcttccccga gttcgcgccg
    11221 gcccaggtcg tgatccagac cgaagcaccg gggttgcatg cggctgccgt cgaagtgcag
    11281 gtcacccgtc ccgtcgagca ggcgatcgcc gggctggcag gcatgacctc gctgcgctcg
    11341 caatcgattc cgggtctgtc ggtggtgact gcgctgttca gcgagtcgtc cgacctgcat
    11401 caaaacaggc agggcgtcgc ggagcgactg gcgcaggccg cgtcgtctct gccacaaggg
    11461 gtggtgccga acatcacgcc gctcacatca tcggccagca cggtactggg catcgggctg
    11521 acctcgacca agctcgaccc catcgcgctg cgcgcgctgg tcgaagacag tctgcgtccg
    11581 cacctgatgg cggtgccggg catcgcagat gtcaatgtct tcggcggcgc gctgcggcaa
    11641 tggcaggtgc aggtcgatac gcagcggcta gcgcagacag gcctttcgct caccgatctg
    11701 gccacggccc tgcgcagcgc caccggtgtg cgagccgcgg gcttcatcga ggcagacaac
    11761 cagcgcatcg cgttgacgat ggagccgcct ggcgccgagc tgggcacgcc ggccgctgcg
    11821 gtgatcgatt cgctgagcct gacggtggtg gggcagcacg gcggccgggc ggtactgctg
    11881 cgtgatgtgg ccgtggtggc cgaaggcggg gctcctgcgt tcaacgcggc acagatcgac
    11941 gggcagcccg gcgtcttcct gatggttcag ggccagctcg gggcgaacac ccgagacacc
    12001 acgcgcgcgc tggactctgc gatagcgcac atccagccgg tgctcgagcg cgccggcgcg
    12061 aaggtacacc tcgacttgtt ccgccccgcc agcttcatcg aaaccgccgt gggcaacgtg
    12121 cgcagcgatg tcctgatcgg cgcaacgctg gtcgtcgccg tgctgttcct cttcctgttc
    12181 aacctgcgca ccgccgtcgt gtctgcggtg gccataccgc tgtccctgat cgctgcagtg
    12241 ctgacgctcc agagcttcgg catcgggctg aacatcatgg tcatcggcgg actcgccatc
    12301 gcgctcggcg aggtcgtcga tgacgccatc atcgactgcg agaacatctt tcgacggctg
    12361 cgcgagaacc gggcattggc agagcctcgc gctgccgcga aagtggtgtt cgacgcctcg
    12421 atggaggtgc gctcgtcggt ggtctacgcg acgttcatcg tggcgctcgt cttcacaccg
    12481 ctgctgacac tgtcgggcgt ggcgggcaag ctgttcgcgc ccctggggtt ggcctacatc
    12541 gccgcaatca tcgcttcgct ggtggtggcg ctgacggtca ctccggccat gtcgctgctt
    12601 ctgctcagac acgctccgct gccggcggcc gatccgccgc tggttgcctg gatgaagccc
    12661 cgctaccgcc gactgctgct tgcgatcgag cgccgcagca cggccgttct gtcagctgcg
    12721 gcgttgtgca tggcggccgg gctcggggtg cttccgctgc tgggcggcga gttcattcca
    12781 ccgctgaagg agggccacta catcgtccac gtcagcgcgg tgcccggcac gtcgctggtc
    12841 gagtcactgc gctttggcaa gcaggtctct gacacgttgc tcgcgatccc cggcgtgaag
    12901 tcggtcgcac agtgggtcgg tcgctcgcag aacggtgcgg acaccttcgg gccgcactac
    12961 agcgaaatgg aagtcgagat cggacaggtc gctggtgcgg agcagcgacg catcctgatg
    13021 gacatacgcc gcgcgctttc cggcgacgac ggcgacgaag accgctccga tgaagaggcg
    13081 gccggcagca cgccgtttcc gggcatggtg ttcggcgtca atacgttcct gaccgagcgc
    13141 atcggcgaga ccgtcagcgg atacccggcc gacgtggtgg tcaccctcta cggccccgac
    13201 ctcgatgcac tcgaccggga cggcaaggcg gtggtcgcca tgctgtcgca gatggcaggc
    13261 gccaccgacg tgcagcttct cgcgccaccc ggcacgccgg agctgtcggt gcggctgcgc
    13321 ccagaacagc tcgcactgca tgcgatgaac gcgggcgagg tgatggattc ggttcggatc
    13381 gcattcgatg gactggtcgt cggtcagcag gccggcgcgg tgacgccgac cccggtcgcg
    13441 ctggtgctgg caccgtcagc gcgccaggac gtgagccagg tggagcgact cccgctgcgc
    13501 acaccatccg ggcggctgat cgagttgcgg gacgtcgccg atgtcgcgct ggtcgaaggc
    13561 cgctacaagg tgctgcgcga aggcggccag cgggtgcaga cggtgatcgc gaacctggag
    13621 cgcgggcacg atcccgaggc gttcgtggcc cagctgcgga cgcggctcgc ccatcacgcc
    13681 aagggcaatg acaagagcgg aggttttgca cccggcaacc acgccgaggt gaccggcgct
    13741 gccgaggcgc aggcgcaggc acgcagcgac ctgctgattg cgtcggcgct ggcgacgttg
    13801 ggagtcggca gcctgctgct gctggccttc ggatcactgc gccacacttt gctgacgagc
    13861 gtcaatctgc cgttcgcact tattggtggc gtggtcgcgg tgctggccac cggaggctgg
    13921 ttgtcactgg gttcggtggt cggcttcgtc acgcttttcg gcatcacgct gcgcaactcg
    13981 atcatgctgg tgtcgcacta tcagcacctg gtcgaggacg aaggccgggc gtggaatctg
    14041 gaaacagcgc tgctgggggc cgaggagcgc ttgccgtcca tcctgatgac tgcattggta
    14101 accggcctcg gattgcttcc cctggcgctc ggcagcgccg aaccaggtcg cgagattgag
    14161 ggtccgatgg cgatcatcat cgtcggcgga ctgatgagct cgaccgtctt gaacctgctg
    14221 gtgctgccga cagtgatgcg gcggtacggg cgatttgggg cgcgtgagct gccgtcgtaa
    14281 aaggcgagcc cacaaacaaa aatgccaacc gaggttggtt ggcatttttg cgtcgtctca
    14341 cgctgcaact tttggttgcg ggggcaggat ttgaacctac gacctttggg ttatgagccc
    14401 aacgagctac cagactgctc caccccgcga ctgagcctgt gagtataact gataaaccgg
    14461 gaccatgggc gaatcttcgc gcccatgatc cgcacaatca ctcggccttg tcgccacctt
    14521 cggcagcagc cacatccgga cggtcaacga gttcaacgaa cgccatcggt gcgttgtctc
    14581 cgacgcggaa gcccatcttc aggatgcgtg tgtagccgcc cggacgtgcc ttgtagcgcg
    14641 ggccgagttc attgaacagc ttggtgacga tgtcgcgatc gcgcgtgcgg tcaaaggcca
    14701 ggcggcgatt ggccagcgtc ggctccttgg ccagcgtgat caggggctcg accacccggc
    14761 gcagttcctt ggccttgggt acggtggtct tgatggcttc gtgctgcagc agcgaattgc
    14821 acatgttgcg cagcatcgcg aggcgatgct cggaggtgcg attgagcttg cgaagtccgt
    14881 gacggtgacg catggtggat ttccttcttt gactgactta tgaaccccag ccgtatcagg
    14941 tactgggggt tgcgatcaag cgcgccaagc gcgcttgtca ttcgatgacg gtccgcacgc
    15001 gagaccgtct gctcctgttt cgacgcgcgt gttaccgctt gtcgaggcct tgcggtggcc
    15061 agttctcgag acgagccccg agggtcaggc cgcgcgacgc cagtacttcc ttgatctcgt
    15121 tgagcgactt gcgacccaga ttcggagtct tgagcaactc ggtctcggtg cgctgaatca
    15181 gatcgccgat gtagtagatg ttctctgcct tcaggcagtt ggccgaacgc actgtcaact
    15241 cgagttcatc caccggacgc agcaggatcg ggtcgaattg cgtgttgcgg ctctgcggct
    15301 cgttgaacgc agcgatttca ctgttctcga gttgagcaaa cacggccagt tgttcgacca
    15361 ggatcttcgc cgaagcacgg atggcttctt ccggagaaat cgcaccgttg gtgttgatct
    15421 ccatcaccag cttgtcgagg tctgtgcgct gttcgacgcg ggcactctct accgtgtagc
    15481 tgacgcgagc cacaggcgag aaggaagcgt ccagcacgat ccggccgatt gacttggtcg
    15541 gctcgtcgcc aaagcggcgc atgttgccgg gcacgtaacc tcggcccttc tcgaccttga
    15601 tctgcatgtc gagcttgccg ccctgcgaca agttggcaat cacgtgattc ggattgatga
    15661 tctcgacatc gtgcggtgtc tggatgtcac ctgcggtgac cgggccttcg ccatccttgc
    15721 gcaataccag agtcacttca tcccggttgt gcaggcggaa cacgacgccc ttgagattca
    15781 gcatgatgtg gacgacatct tcctgaacac cgtccaccgt ggagtactca tgcagcacgc
    15841 cagcgatcgt cacttcggta ggcgcatacc cgaccatcga cgacagcagg acgcggcgca
    15901 gcgcgtttcc gagcgtgtgg ccatagccgc gttcaaacgg ttccagcgtg accttcgcac
    15961 gattgacacc gagcggctcg acgttgatgg ctttgggctt gagcaagttg gtttgcattc
    16021 ggtctttctt tcaataccct cggctcgtta cgccgataag gctgatgaac ccgtcggtgc
    16081 cgggataccc gtcaacaccg atctcgggga atacccaccg cgccacgcac tgcaaacccg
    16141 aaggcctgcg gcgcgcgccg caaccggatc aacgtgaata caactcgacg atcagggctt
    16201 ccttgatgtc ggcgccgaat tcgtcacggt cgggtgtctt cttgaacacg ccttccatct
    16261 tggccgcatc gacctgaatc caggcgggaa gtcccatcga ctccgccagc ttcagcgaat
    16321 cggcgatacg caactgcttc ttggcctttt cgcgaacgct caccgcgtca ccgaccttga
    16381 ccatgtatga agcgatgttg actacaccac cattgaccgt gatggacttg tgcgacacca
    16441 gctggcgcgc ctcagcacgg gtcgagccga agcccatgcg ataaacgacg ttgtccaggc
    16501 gcgactccag cagcatcagc aggttggcgc cggtgttgcc cttgcggcgc tcggcttcgg
    16561 cgaaatagcg cctgaactgg cgttcgagca cgccgtacat gcgcttgacc ttctgcttct
    16621 cacgcaactg cagcccgaag tcagaggtgc gcgacccgga ggtgcgtccg tgctgacctg
    16681 gcttgctgtc gaatttagcc ttgtcgccga tcggacgacg ggcgctcttg agaaacaggt
    16741 ccgtgccttc acgacgggac agtttcgcct tgggaccgag ataacgtgcc acgttgattc
    16801 cttgtttatc tgtcttacgc aggctgccgt tttccaacag cggcttgcgc gagtctggcg
    16861 agtgttgtta tggggcgctc agacggtggg cttagcgggt gtcggtcgat gcgaaacatc
    16921 gtccgatccc aacaaaaatg tcggcgctgc cccgaggcac cgccgacacg ggtaaaacga
    16981 actcagattc gacggcgctt ttgcgggcgg cagccgttat gcggcactgg cgtcacatcg
    17041 gaaatcagat tgattcggat cccgagcgat gccaaggcgc ggaccgaaga ctcgcgcccc
    17101 ggacctgggc ccttgatgcg cacatccaga ttcttgatgc cctgctcctg agcggcgcga
    17161 ccagccactt cagcagcaac ctgcgctgcg aacggggtgc tcttgcgcga gcccttgaaa
    17221 ccctggccgc cactggacgc ccacgacaag gcaccgcctt ggcggtcggt gatcgtgatg
    17281 atcgtgttgt tgaaagaagc gtggacgtgc gcgatgccat cggccacgtt cttgcgagct
    17341 ttcttgcgca cgcgctgtgc ggcggtattg ggtgctttgg ccatgtctgt tcttccttaa
    17401 acgtgtgctg tcgcgcgtct gcttttgccg gacaggaaca tcacttcttc accagcgcgc
    17461 cggacttgcg cgggcctttg cgtgtacgag cattggtctt ggtgcgctga cctcgcaccg
    17521 ggaggccacg ccgatggcgg aacccgcggt agcagcccag gtccatcagc cgcttgatgt
    17581 tgatcgacat ctcgcgacgc agatcgcctt cgatcgtgag gcgaccgatc tcgtcacgca
    17641 ggcgctcgag gtcggtgtcg gtcagatcct tgatcttccg gtcaaacggc acaccgctgt
    17701 tgacacagat tttctgtgcg gtggtgcgac cgattccata aatggacgtc aaaccaatct
    17761 ctgcgtgctt gtgcggcgga atgttgatac cagcgatgcg tgccatgggc ttcctctaat
    17821 ttgcttgtcg tcgggggatg tgaatcaacc ctggcgctgt ttgtgacgcg gatcggtgca
    17881 gatcacgcgc acgactccct tgcgccgaat gactttgcaa ttccggcaca tcttcttaac
    17941 ggatgcagca actttcatga tgttctcctt ggtccgatcg ggaccgcctc agcacttaaa
    18001 caatgtagcc cgtcagactt atttcgctct gaacacgatg cgcgcccgac tcaggtcgta
    18061 aggtgtcaac tccacagtca ccttgtcgcc agggagaata cggatgtaat gcattcgcat
    18121 ctttccggaa atgtgtccca gcacaatgtg cccgttttcc agtttcaccc ggaacgtcgc
    18181 attgggaaga ttttcaagaa tctccccttg catctgaata acgtcatctt tcgccatgcg
    18241 gtccgatacg tgataccaag ctgctctcga ttgcctcgcg acaaccggat cagctcggct
    18301 tgaaattcgc tttcttcaat aacgattcgt actgctggct ctgaacatag ctttgaacct
    18361 gagcccagaa atccatcgtc accaccacga tgatcaggag cgacgtgccg ccgaaataga
    18421 acggcacgtt gtacttcaac accagaaact caggcagcag acacaccagc gtgatgtaga
    18481 ccgcaccggc caacgtcaac cgcgacagga tccgatcgat gtgccgcgcc gtctgatccc
    18541 ctggacgtat gccaggaatg aaggcgccgc tcttcttcag gttgtccgcc gtctcgcgac
    18601 tgttgaacac caacgccgta tagaaaaagc aaaagaagac gatcgcagtg gcatagagga
    18661 gcacgtaaat cggctgccca ggtgatagca aaccagccag gtccttcaac cagcgcaacc
    18721 catctcccgt ggcaaaccaa ccgaccacag tcgcgggcaa caggatgatc gaggaggcga
    18781 agatcggagg gatcacacca gacatattga gcttcaaagg caggtgcgag gactgtccac
    18841 cgtaaacctt attaccaact tgtcgcttgg cgtagttcac cagaatcttt cgctgtcccc
    18901 gctcaacgta cacgacagcg aacgtgacaa agccaacgac aaccaagatg aaaagacagg
    18961 cgacatagct catcgaccca gtgcgaacca gctcgaacaa gccaccgatt gcgctgggca
    19021 ggcctgcagc aataccggcg aatatcagga tcgagatccc gttgcccaat ccgcgctcgg
    19081 tgatctgctc acccagccac atcaggaaca tggttccggc caccagactc accaccgccg
    19141 tcaggcgaaa tccaaatcca ggcgaaatca cgagaccttg cgatccctcg agagccaggg
    19201 caataccgag cgactggaag atcgccagac ccagcgtgcc gtagcgggtg tactgcgtga
    19261 tcttgcgtcg gcccgcctca ccctctttct tcaacgcctc cagcgtagga accacgtagg
    19321 tcatcaactg catgatgatc gacgcggaga tgtacggcat gatgccgagg gcgaaaaccg
    19381 tgaaccgcga caacgcgcca cccgagaaca tgttgaagag actcaggatt ccacctgcct
    19441 gccccttgaa cagctccgcc agttgattgg gatcaatgcc agggaccggg atgtgcgcgc
    19501 ccatccggta cacgaccagc gcgagcagca agaacgtcaa ccggcgacgc aggtccccga
    19561 atttgccgct cttggcgagt tgattcgcgt tggttgccac agggtccgtt cagtcgtact
    19621 ggatcaaatc gccagcgaga tcaggccacg gagccgccgg ctgcctcgat ggcagccttc
    19681 gcgccggcgg tggcgccgac acccttgaga gtcacggcca gggtcagttc gccagtcttg
    19741 atgaccttga cgaccttggc gatttcaccg accagaccgg cctgcttcag cgtgagcaga
    19801 tcgacctcgg ccacacccag gcgttgcaga tcggtcaggg tgacctccgc gttgtacttc
    19861 agcgaagccg acttgaagcc tcgcttggga agtcgacgtt gcagcggcat ctggccgcct
    19921 tcgaagccga ccttgtgata gccacctgcg cgcgactttt gacccttgtg accacggccc
    19981 gcggtcttgc ccaggcccga gccgatgcca cgaccgacac ggcggcgctg acgtttcgag
    20041 ccctctgcgg gcttgatggt gttgagttcc atggtcatct tttccgggtc agtctcagag
    20101 taccttgagc aggtacgaga tcttgttgat catgccgcgc actgcgggcg tgtcttcgag
    20161 ttcggactcg ctgttcagct tgcgcaaccc gagcccgcgc acggtggcac ggtgtgattc
    20221 gcgggtgcca gcgatgctgc ggaccagctt gatcttgacg gtgggtttat cagccatttt
    20281 tttctccagc tcgatgtcag ttgaagatcg cttcaaccgt caagccgcgt ttcgcggcca
    20341 cttcggcagg ggtgctggtg tgcttcaagg catccagcgt cgcccgaacc aggttgtagg
    20401 ggttcgacga gccgaggctc ttgcagacaa tgtcggtcac gcccatcact tcgaacaccg
    20461 cgcgcatcgg gccaccggca atgatgccgg taccagccgg agccggcgcc atgaggacgt
    20521 gggaagcgcc atgctcaccg accaccttgt ggtggatcga gccgttcttc agcgacacct
    20581 tgaccatgtt gcggcgagcg gcctccatcg ccttctggac cgccacaggc acttccttgg
    20641 ccttgccctt gcccatgccg atgcggccat cgccatcacc gaccacggtc aacgctgcaa
    20701 agctgagcgt gcgtccgccc ttgacgacct tggtgacgcg gttgaccgcg atcatctttt
    20761 ccttcaggcc gtcgtcattg ccttcggtct gtgcgcgggg ttgaaacttt gccattttcg
    20821 aattccttgc ttagaactga agaccggctt cacgagcggc ttcagccaac gccttgacgc
    20881 gtccgtggta tgcgaaaccc gcccggtcga aagcgacttt ctcgacgccc gcagccctgg
    20941 ccttctcggc gatccgcttg ccgatcagcg ccgcagcgcc gacattgcca cccttgcccg
    21001 ccgcgcccag ttccttgcgg acatcggctt cgacggtcga cgcgctggcc aacacccgat
    21061 tgccatcgtc agaaatcacg ctggcgtaga tgtgcaaatt ggtgcggaag accgtgaggc
    21121 gaaccacccg ctgccgggca atgcgaaccc gggtctgacg cgagcgacgg gtgcgctgat
    21181 cttttttgtt caacatgtca tccgctcctt acttcttctt ggtctctttg atcgtgacct
    21241 tttcgcctac gtatcgaatg cccttgccct tgtagggctc cggcggacga atggcgcgga
    21301 cttcagcggc gatctgaccc accacctggc ggtcagaacc cttgatgacg atttcggtct
    21361 gtgtcgggca ctcgaccttc acgccatcag gcatgtcctt gcccaccgga tgcgaaaaac
    21421 cgatctgcag attcaacttg ctgccagcag cctgcgcgcg gaaacccacg ccgaccagcg
    21481 acagccgctt ttcgaagccc ttgctaacac cgttgaccat gttcgctacc agcgcgcgca
    21541 tcgtgcccga catcgcatcg gccgcggtgg tggcatctgc gggaatgaat tccagcttgc
    21601 cagcggtatt gctgatattc accagcgggt tcgccggacg caccagcgtg ccaagcgggc
    21661 ccttcacggt gatttgctct gcggtgatag caacgtccaa gccttgaggc acggacaccg
    21721 gcatttttcc aactctggac atgtgcggac tcctctctta ggccacgtag caaagaactt
    21781 cgccaccgat gccgacttgc cgcgccttgc ggtcggtcat cacgccacgg ggggtggtca
    21841 cgatcgctac gcccagaccg ttcttgacgt tcggaatgtc gtgacggccc ttgtagacgc
    21901 gcaagccggg acggctgaca cgctcaatgt gctcgatgac cggcttgccg gcgtagtact
    21961 tcagcgcgat ttgaagttgt ggcttggtgg cctcgccaac cacggcgaag ctatcgacgt
    22021 agccctcgtc cttcaacacc tgcgcaatcg ccaccttcag cttcgacgac ggcatcgaca
    22081 ccgatggctt ttccaccatc tgcgcgttgc ggatgcgggt cagcatatcg gcgataggat
    22141 cactcatgct catacgaata tctcctgatc gacgcgccgc ttaccagctc gccttgatga
    22201 caccgggaat gtcgcccttg aaggccaatt cacgaatctt gttgcggccg aggccgaact
    22261 tcctgaaggt gccacgaggg cggccggtca gggcacagcg attgcgctga cgggtcgggt
    22321 aggcattgcg cggcagcttc tgcagttcga gtcgggcggc gtagcgctcc tcgtcggact
    22381 ttttcgcgtc gttggcgatg cccttgtact cggcgtactt cttggcgtac ttggcagcga
    22441 gctgctcacg cttgagttca cgctggatca gtgaaagttt tgccacggtc agcctcagtt
    22501 cttgaatggg aacttgaatg cggccagcaa agccttgcac tcgtcgtcgt tcttggcggt
    22561 ggtggtgatg ctgatgttca acccgcgcag cgcatcgacc ttgtcgtact cgacctcggg
    22621 aaagatgatc tgttctttca cgccgatgtt gtagttgcca cgaccatcga acgagcgtcc
    22681 cgaaatacca cggaaatcgc gaacacgcgg caaggcgatc gtgacgaagc ggtccaggaa
    22741 ttcatacatc tggacgccac gcaacgtgac catgcaaccg atcggaacgc catctcgaat
    22801 cttgaagccg gcgatagcct tcttcgactt ggtaaccact ggcttctggc cggcgatctt
    22861 ggtcatgtcg ctgacggcat gttccatgac cttcttgtcc gacaccgcct cgctcacacc
    22921 catgttgagc gtgatcttgg tgatgcgggg aacctccatc gcggacttgt agccaaactt
    22981 ggcaatcaag ccaggagcaa tggtgtcccg gtatatagcc tgaaggcggg cgggttgttg
    23041 ttgagccatg tcggaccttt aaaccttgat ttcttcgccg ctggacttga agacgcgaac
    23101 cttcttgcca tcagccagaa gcttgatacc aacccggtcg gccttgccag acgccgagtt
    23161 gtaaatcgcg acattagact gatgaatcgg catcgtcttc tcgacaacac cgccagtcgt
    23221 gcccttcatc ggattcggct tcacgtgctt cttgacgatg ttcacaccat cgatcgtgat
    23281 gcggtcttca tcaatgcgag ccgcaacgac gccacgcttg cctttgtcgc gaccagtcaa
    23341 gacgatgacc tgatcaccct tgcgaatctt gttcataagc tcgttcctgt ctcagctcag
    23401 aggacttcag gcgccagcga gacgatcttc atgaaacgct cggtgcgcaa ctcgcgcgtg
    23461 accggtccga agatgcgcgt gccgatgggc tccaacttgg cgttcagcag aacagccgcg
    23521 ttgccatcga acttgacaag cgagccatcc tggcgacgga cgcccttggc cgtacggacc
    23581 accacggcgc tgtagacctc gcccttcttg actcgcccac ggggcgcggc ttctttgatg
    23641 ctgaccttga tcacatcacc gattccggca taacgccgac gggacccgcc caggaccttg
    23701 atacacatga cggacttggc gccagtgttg tccgcgacgt cgagtcgcga ttgcatttga
    23761 atcatttttc aatgtcccaa cttgtaccgt tgtcgcgagg gcaaagcccc acagcatcga
    23821 tcagtcttgg gcccgtggcc cccgcgcaac gaattgcgcg aaagcagttt tgggcggaac
    23881 cggaagcaag gctgacaaat cagccgctga ttccagcgaa gtcgatgatg gtagcaggta
    23941 gcggcactcg ccgtcaacct cctcaccacc ttcctgacaa aacattccct cgcccgctgc
    24001 gagcagcgga actcttcaga cttcgcgagc cttctggagc agcttcgtca ccacccacgc
    24061 cttggtcttc gagatgggac ggctttcaga gatctcgacg acgtcaccgt tcttgtattc
    24121 gttgttctcg tcgtgtgcgt ggtacttgct cgtcttggca acgatcttgc cgtacagctc
    24181 gtgcttggtg cgacgctcga tcaggacggt gatcgtcttg ctgcgcttgt cactgacgac
    24241 cttgccgacc aaggtacgaa cagtcttttc cttgacggcg ggttgagctt cggtcatttc
    24301 gcagctcctt tcacttcacg ttgcttctgg gcaatcacgg tcttcgcgcg agcgatgtcc
    24361 cgccgcgtgt tgccgagttg ggtgtggttg gccaactgct gggtggcctt ctgcatgcgc
    24421 aggccgaaat gggccttcag cagatcgctg acctctttct ggaggccggc gatgtccttg
    24481 gcgcggagtt cagatgcttt cacttggagt tctccgtggg gtgtcaggcg ccgacctggc
    24541 gtgcgacaaa cgtgcagctc agcggtagct tcgcagctgc gagcgtgaaa gcctcgcgcg
    24601 ccaacgcttc cggaacgccg ttgatctcgt agagcacctt gccgggctga atctcggcca
    24661 cgtagtactc cgggttgcct ttgccgttgc ccatgcgaac ttcggccggc ttctgcgaga
    24721 tgggcttgtc aggaaagatg cggatccaga tgcgtccgcc gcgcttcacg tgacgcgaaa
    24781 tcgcgcggcg cgcggcttcg atctggcgtg cagtcaagcg accgcgctcg gttgccttca
    24841 gtccgtactc gccgaacgac accttggcgc cacgggtggc aacaccggtg ttgcggccct
    24901 tttgttcttt gcgatatttt ctgcgtgcag gttgcagcat gcttattctc ctttggcctc
    24961 gccctgcgtg ggcggaacgg ctttgcgcac gcgcttgacc gtgggggcct tgctcggatc
    25021 accagcttcg gacggcttgg ccgccaagtc ggctggacca gccgcgcggg gaccccgatc
    25081 ggcacctggt gcaccaggcc gaccgcgact aggcggcgcg cccggacggg cgttgcgacg
    25141 cggccggcga tcgtcctcac cttccggctt gttgacgaca cccggcgctt cgccatgacc
    25201 caaacggtcg ccgcggtaga cccagcactt gacgccgatg attccgtagg tcgtcttggc
    25261 ttcggaaaaa ccgtagtcga tatcggcctt cagcgtatga agtggcactc gaccttcgcg
    25321 ataccactcg caacgggcga tttcaatgcc gttcagacgg ccagacgaca tcagcttgat
    25381 accttgagca cccagccgca tcgcgttctg catggcacgc ttcatcgcac ggcggaacat
    25441 gatccgcttc tcgagctgtt gcgtgatgct gtcggcgatc agctgtgcat cgatctcggg
    25501 cttgcgcacc tcttcgatat tgactgcgac tggcacaccc agacggcgag tcagttcggc
    25561 cttcaggctt tcgatgtcct cgcccttctt gccgatcacg acacccggac gcgccgagta
    25621 gatcgtgatg cgggcgttct tggcagggcg ctcgatcaac acgcgcgaga ccgccgcgtt
    25681 cttcaggcga gccttgagga actcgcgaac cttcaggtcc tcggccagca tcgtggcgaa
    25741 attctggttg ttcgcgtacc agcgcgacgc ccagttgcgc gtgaccggca gtcgaaagcc
    25801 gatggggtga atcttttgtc ccatgatctt tgcctcagtt gccgacggtg atgtaaatgt
    25861 gcgcagtcgg cttgcagatg ctgttgcccc ggcccttggc acgagccgag aaccgcttca
    25921 ggatggcacc ttgctcgaca tagattgtct tgaccttgag ttcatcaatg tctgcgccgt
    25981 cgttgtgctc ggcattcgcg atggccgatt caagcgcctt cttgatgatg ccggcagcct
    26041 tcttgggcgt gaatgtcagg atgttgaggg cttgatcaac aggccggcca cggaccaggt
    26101 cggcgacgag tcgcccctta tcggcggaca ggcgaacgcc acgaacgatt gaacgggttt
    26161 ccatcgtggt tccttatttc ctcgcggcct tcttgtcggc cgggtgtccc ttgaacgtgc
    26221 gtgtcaacgc aaattcgccc aatttgtgcc cgaccatctg gtcggttaca tagacaggga
    26281 tgtgctgctt tccgttatgg acggcaatgg tcaacccgat gaaatccggc aggatcgtag
    26341 aacgacgcga ccaggtcttg atgggcttct tgtccttgat ggcaaccgct ttgtcgactt
    26401 tggccatcag atgatggtcg acgaacgggc cttttttcag cgaacgggtc atgaggcggc
    26461 ccctttactt cttgcgacgc gaaacgatga acgtctgcgt gcgcttgttg ttgcgagtgc
    26521 gatagccctt ggtcatcgtg ttccatggag acaccggaac ctgaccctcg ccagtgcggc
    26581 cttcgccgcc accgtgcggg tgatccaccg ggttcatggc gacaccacga acggtcggac
    26641 ggatgccctt ccagcggatg gcaccagcct tgccgtactg acgaaggctg tgctcctcgt
    26701 tggagacctc accaatcgtc gcgcggcagt cgatgtggat ccgacggacc tcgcccgagc
    26761 gcatccgcac ctgcgcgtag gttccttcac gagccagcag cgtgcaagaa gtgccggctg
    26821 atcgggcaat ctgagcaccc ttgcccggca gcatttcgat gcagtgaatg accgaaccaa
    26881 ccggaatgtt ccggatgggc aaggtgttgc ccgccttgat cggcgcttca gcaccgctca
    26941 gcagcgtttg accgatttca aggccgcgcg gagcgatcac ataccggcgc tcgccatcgg
    27001 cgtagcacac cagcgcgatg tgagccgtgc ggttcgggtc gtactcgata cgctcgacct
    27061 tcgccgggat gccatccttg ttgcgcacga aatcgacgac acgataatgg tgcttgtgac
    27121 cgccaccctt gtgacggatc gtgatgtggc cgttgttgtt gcgacctgcg tgctggaact
    27181 gcggctcaag cagcgatgct tccggcttgc ccttgtggag atgcgagtgc accaccttca
    27241 ccacggcacg gcggccaggc gatgtcggct tgactttgac gacggccatt acgcggcctc
    27301 cccagagatg ttgagttcct gtccggcctt caacgacacg tacgccttct tgacatggtc
    27361 acggcgcccg atcgatcgac cgaacttctt gaccttgccc ttttgcagca ccgtttgaac
    27421 ggactcgacc tcgaccttga acatcaattc gacagcagcc ttgatttcag gcttggtcgc
    27481 atcgcgaagt accttgaaaa gaacttggtt gtgcttttcg gatgccatcg tggctttttc
    27541 gctgacgatc ggcgcaatga ggatctgcgc caaacggttt tcagcatgtt tgacaggggc
    27601 gctcatgcca gcatctcctt gagttgctcg atggcagcct tggtgaccag caccttcttg
    27661 tagtgcacca gggacagcgg atcggcgtga cgaggctcaa ccacgagcac attgcgcaag
    27721 ttgcgcgatg cgagcagcag gttgtcatcg acctgatcgg cgatcaccag caccgagtcc
    27781 agccccatgg ccttcaactt ggcagcgaac agcttggtct tcggggagtc gacttccatc
    27841 gaatcgatca ccgccagacg gccttcacgg gccagctgcg aaaagatcgc ggccataccg
    27901 gcgcgataca tcttcttgtt gaccttctgg gtgaagtttt cgtcggggct gttcggaaat
    27961 atgcgaccgc ccccacgcca cagtggcgac gaggtcatgc cggcgcgggc acggccggtg
    28021 cccttctggc gccacggctt cttcgtcgag tgcttgactt cagcgcggtc catctgggca
    28081 cgggtgccct gacgcgcgtt ggcctggaag gccacgacga tctggtgcac cagcgcttcg
    28141 ttgtaatcgc gagcgaacaa ggtgtcgggc gcgtcgactt tcgacgtggc ctgaccttgc
    28201 gcattgagga gctcgagctg catcagttgg ctcccttctt gaccttcacc ttgacggcgg
    28261 ggctgacgac gatgtgaccg ttcttcgaac caggcaccgc gccacgcacc agcaacagct
    28321 ggcgggcctc atcgatgcga atgatgtcga ggttctgcgt cgtcttggtc acgtcgccac
    28381 ggtggccgga catcttcttg cccggaaaca cacggcccgg atcctgcgcc atcgagatcg
    28441 agcccggcac gttgtgcgaa cggctgttgc cgtgcgacgc gcgctgcgag ctgaagttgt
    28501 ggcgcttgat ggtgccggtg aagcccttgc cgatggacgt gccctgcacg tcgaccagct
    28561 ggcccacggc gaacgtggtc accgggatct gcgcgccagg cttgaagccg gcagccacat
    28621 cggcggcgac acggaattcc ttcaggatct ggccggcttc gacgcccgct ttcgcgaggt
    28681 gaccagcttc gggcttgttg actcgggacg ccttgcgcgc tccgtaggcc acctggatgg
    28741 ccgtatagcc gtcggtttcg tcggtcttga cctgggtgac gcggttgttg gacacgtcga
    28801 gcaccgtcac ggggatggcg tcgccatcgt ccgtgaagat gcgcatcatg cccaccttgc
    28861 ggcccagcaa accgagacgg ttgctcagac tcatgtgttt gctccaatgc cccacatcga
    28921 ttggcggggc tgatgagttt ggcagcgcac atcaggtgcg ctgccgggaa agcctgcgat
    28981 tctactgcag cttgatttcc acgtccacgc cggccggcag atcgagcttc atcagcgcgt
    29041 ccacggtctt gtcggtcggg tcgacgatgt ccatcaggcg ctggtgcgtg cggatctcga
    29101 actggtcgcg gctggtcttg ttgacgtgcg gcgagcgcag gatgtcgaag cgctgcatgc
    29161 gcgtcggcag gggcaccggg cccttgacga tcgcgccggt gcgcttggcg gtgtcgacga
    29221 tttcgagtgc cgactggtcg atcaacttgt agtcaaacgc cttcagacgg atgcggatct
    29281 tttgcttttg catgacggtt ccttaaagag cagtgcggcc ggcttgcgcc tccaggccgc
    29341 gttgattcac gaaggccggc agcagcggtg ctgactgccg gtcgtcacgc gttcactcga
    29401 tgatcttggc gacaacgccg gcgccgacgg ttctgccacc ttcacggatg gcgaagcgca
    29461 ggccctctt
//
LOCUS       DAOORV010000028        29090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1864_length_29090_cov_7.462132, whole genome shotgun sequence.
ACCESSION   DAOORV010000028 DAOORV010000000
VERSION     DAOORV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 29090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_29090_cov_7.462132"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2460)
                     /locus_tag="PKV56_05785"
     CDS             complement(<1..2460)
                     /locus_tag="PKV56_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX67358.1"
                     /translation="MNFLTSFLGRRWRAASLGRLPWLIGAIGLFLGVSRAMDHWSICW
                     PIGLQGLHGAASESLSIVSAMALWPLIPKMLRTASVERLQSLIGELEAEVRQRRSNEE
                     QLVQHQDNLSVTLASISAGFIATDREGQVTSMNAVAERLTGWVQADARGRPVGEVLQR
                     HESSLSDLSRNPVDAMIERGITIDVVDRFIVLSRDGVRAPVELQAGLTRANDGTVLGM
                     AMVIRDRTGVIRAEAEPSRLAAIVESSSDAIVGKTLDGRITDWNAGAEALFGYSAGEA
                     IGQSVTMLIPPERAEEELRIVANISSGGRVPRFDTVRLAKNGRRIDVSVTVSPIRDGE
                     GRIVGAAKIARDVTSERRAEAALRDSETRLRFTLESAAIGDWDMDLATGVMQRSLRHD
                     RCFGYDEMQPEWTFDTFIEHVHPDERSEVAHSLHVALTSAQPWHVQCRVIWPDGSVHW
                     ISAHGKVQLQEGHAGRMLGIVTEITQQRLAEDVRLTAQRLEVENRQIQAANRLKSQFL
                     ANMSHELRTPLNAVIGFADLLQSGTVPPGSPKHAKFLGHISTSGRHLLQLINDVLDLS
                     KVESGKFEFFPEPVQLPALLKEVSDVLQATTQRKQIEIDVQVEPGLTDLVLDMARLKQ
                     VLYNYLSNAIKFTPQGGRVTVRAMAHGASHFRIEVEDTGIGISPTDLSRLFVEFQQLD
                     AGYSKQHQGTGLGLALTRRLVEAQGGSVGVRSTVGVGSVFHVLLNRVHGTDAQGASLG
                     KAAARNRMLLIEEHGPASALALQSLSEIGFDVDCAATGEQALSKAGTTVYDAMTLSLQ
                     LADRTGLGVLEGIRQDGASRYS"
     gene            2658..3626
                     /locus_tag="PKV56_05790"
     CDS             2658..3626
                     /locus_tag="PKV56_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOX67359.1"
                     /translation="MTTIPEPASEHPEPVRIHIQEVCVRDGFQIEPVFVPTERKVALI
                     NALGRTGLSKIEVTSFSSPKAIPALADAEEVMRRIDRIPGVEYAALVPNVRGAQRALA
                     CQVDELNLVMSASASHNQANLRMSMEQSLAQFADIVAAVDGQARINASLSTAFGCPFD
                     GEVPAARVLELVQRFVDLGITRVALCDTTGMANPAQVRRLFSAVIERWPAVTFTAHFH
                     DTRAMGLANALAALEAGVRHFDASLGGLGGCPYAPGASGNVCTEDMVHMFEAMGCDTG
                     VALEPLLTLARSLPDIVGHPVPGQVMKAGPATRRYPLGSPGSAQPA"
     gene            complement(3623..3904)
                     /locus_tag="PKV56_05795"
     CDS             complement(3623..3904)
                     /locus_tag="PKV56_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOX67360.1"
                     /translation="MNPTFQEFEAASRAQGFDEVLERTWPPLTVLETHVHPFALQAVV
                     VRGEMWLSVGEASRHLTAGDTFALEADVPHAERYGAEGATYWVARRAAA"
     gene            complement(4012..6444)
                     /locus_tag="PKV56_05800"
     CDS             complement(4012..6444)
                     /locus_tag="PKV56_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX67361.1"
                     /translation="MNLMDTGLGRLFFWPGMRLMRAMKFPAKITLISVVLMVPLTWLT
                     VTALLASHASLESTRQEARGNPLIGLSLDVIAQTQKHRGLVNRALAGDTAVAGDLAQN
                     RAAMKSAIGALQSLVIQHPEFDLGPDWQPIRRSLERLAEGDIPVQASESFALHSQQVE
                     AMRRFVSRAAEASGLLLDPEGAPFHLMHLSVEPLAVWTEALGQMRGRGAALLRTGEAS
                     AIDRAEIIAQARLLNHALVSAEDIVGALRRAGEAPPKGLSEALAAARDYAQQAEATFT
                     APKLVGDPASYFDAGTAAIGQALQIGKVATDRLQFLLDERAARLQTLWFVQLAVGLCT
                     VLGAAYLAIVFFRTSFGAIRVLQGSVTQLAAGDFATHIRLRGTDELSVVGHSLDDMTG
                     RISEMVADVRSNSSMVAQAGLSLAADTKALSERTEAQASSLEQTTASVQELSGAVRKN
                     AQGAEAASSMAARVQTIAENGGSAIQSAVVAMQDIQTSSSRVQEIVGVIEGISFQTNI
                     LALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSSESAREIKSLIAASAGHVNTGVAQI
                     GGASQTFAEIVQGIREVASRVHEIKTSTVEQSSGLDQIAQAVQHIDEITQRNAQMVES
                     AMNSSSQLSGRAERLSSAVSNFKLRQGSADEALALVRQAVEMYRRNGHSALEQITDAA
                     NGLSDRDMYVFAFDRGGIYRAFAGKADKVGTSVREVPGVDGDKLVRDAFDQAAHGGGW
                     VDYQFANPQTKATDLKSSYVEAVTPDLVLGCGVYKSRGKASGALVIDMSMQGLREQQR
                     LKLADVLPGAAA"
     gene            complement(6541..7350)
                     /locus_tag="PKV56_05805"
     CDS             complement(6541..7350)
                     /locus_tag="PKV56_05805"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOX67362.1"
                     /translation="MSASLLSTQHQFQQHMLGAGDASRLIAGDAARRQLGLRIYANAY
                     RQRLLETLIDSYEKTCLVMGEAAFEAAALDYIAAHPPTTRSLRWYGEQFAEHLLAQGD
                     AELPASSAWSELARLDWALRSAFDGPDSGVLDAGTFADLAESAWATLRLVPVPTVQLR
                     VFRHNTVAVWQAIDDDQDPPETQASVNGVDWLVWRKGLQPHFRSLHSTEAALLRAMLA
                     GSTFAEACAQAEECIGNRSDVAASGVPDMEMLVGGFMRQWLEDQLLSGIFV"
     gene            complement(7347..8216)
                     /locus_tag="PKV56_05810"
     CDS             complement(7347..8216)
                     /locus_tag="PKV56_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOX67363.1"
                     /translation="MSDAQPRLANPGFGLGLRTPHYSDFLESPQRVDWLEILSDNYLV
                     PGGKPLVMLDAIRADYPVVMHGVALSIGAADGPDRAYLAALAALARRVEPLWISDHLC
                     WTGVHGRVLHDLYPLPYTDASIDVVVRNVHIAQEVLKRPLVLENVSSYVSFESSEMTE
                     WEFITEVCQRTGCRLLLDVNNIHVSSVNHGFDARAFLRGVPADRVQQIHLAGHTDHGD
                     HLIDTHDHPVADPVWALYAEALARLGPVATMIERDDRIPPLAELVCELDRAREIAADA
                     LETTRREPVRSCA"
     gene            complement(8232..8513)
                     /locus_tag="PKV56_05815"
     CDS             complement(8232..8513)
                     /locus_tag="PKV56_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67364.1"
                     /translation="MFNASRGFQIASAAAALALSATAFAAEAPKGSSGKAIAASDKVH
                     CYGVHSCKGNSDCKTAEHSCKGQNACGGHGFKGMAAAECLKAGGTIGDL"
     gene            complement(8662..9195)
                     /locus_tag="PKV56_05820"
     CDS             complement(8662..9195)
                     /locus_tag="PKV56_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOX67365.1"
                     /translation="MNQPHSASLWSRLWTLGETAIRGLEALQPAAQLAARWFIAGVFF
                     RSGLTKIADWETTVALFTDEYHVPLLSPELAAALGTSAELALPVLILFGFAGRFAAAG
                     LFILNIVAVISVEDMPIPALQQHIFWGSVLVGLLLWGPGRWSVDRFVWPRLRALVVGA
                     PPVDAASAAGPASSRRI"
     gene            complement(9220..10080)
                     /locus_tag="PKV56_05825"
     CDS             complement(9220..10080)
                     /locus_tag="PKV56_05825"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOX67366.1"
                     /translation="MTAGDEPAASAATSAESERQRALIAALWTADAPQAARDLPCVDG
                     PVPVARGLAAYRANAGAHAARALEGACPTVQALIGSDDFRRLAREHWMQALPTSGDLA
                     EWGGALGAGLEAHPELLEWPYLGDCARLDWAVHQCERAADAVFDAGSISRLGDTDPRH
                     LHLELRPGTAVLRSRWPVVLIHDAHRQADTKSALAAVSEALAQGVGEIALVARSGAGS
                     GWRAQVHRIDAATALWTERLLAGDSLSAALGAVDAAADADRDIAFDLGTWLATALREG
                     WLTEIRVCSD"
     gene            complement(10077..10943)
                     /locus_tag="PKV56_05830"
     CDS             complement(10077..10943)
                     /locus_tag="PKV56_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOX67367.1"
                     /translation="MSALPPVCRELAGIGLRQPHYAQVFEDPPAIGFVEVHSENFYAD
                     GGAALAVLDEARSRYPVSLHGVGLSLGSAAGVDAGHLDRLDRLVRRIEPALVSDHASF
                     ARAPREPGGPVVHANDLLPIAFTPASLSILCDNVSRVQDRLQRQMLVENLSAYLTFAD
                     NTLTEPEFLAALCRRTGCGLLLDVNNLMVNALNERVADPLAHGCAFIDALPPGIVGEV
                     HLAGYCQLPDIVIDDHGSLVRPAVWALYEHALQRFGAVPTLVEWDTDVPALDVLLGQA
                     RQATERLARVRR"
     gene            complement(10981..11259)
                     /locus_tag="PKV56_05835"
     CDS             complement(10981..11259)
                     /locus_tag="PKV56_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HOX67368.1"
                     /translation="MNHRLIASSALASVLALGMLSTASAADAPAKEKCFGIAKAGAND
                     CANLSGTHSCAGQAKADNAADEWKYVAKGTCASLGGKSADEAKKAMKK"
     gene            complement(11448..12698)
                     /locus_tag="PKV56_05840"
     CDS             complement(11448..12698)
                     /locus_tag="PKV56_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HOX67369.1"
                     /translation="MSTIELIAAVIFAIALLHTFSVKFFERLAHRSPRNAGLFHLLGE
                     IEVVFGFWAFVLIAFMAAVEGGQKAIDYAESRQYTEPLFVFVIMVVAASRPVLETIRA
                     LIESVARLLPIRTAVARVWLCLAAVPLIGSLVTEPAAMTLAALMLAPLVFRGDVPEGL
                     KYGALGVLFVNVSIGGTLTSFAAPPVLMVAATWQWDSAYMASHFGWKAAIAVVINATA
                     VTALLSRHLTEPAKPTSDESGERIPLVVTLIHLGALSMVVTFAHHPVIFLGVFMLFLG
                     FTQAYARHQSPLILKEALLVGFFLAGLVVLGGMQRWWLQPIVSSLEPTALFFGALGLT
                     AITDNAALTYLGSLISGMSEQAKYMLVSGAVAGGGLTVIANAPNPAGVTLLKHGFEDQ
                     SVGAGGLLLGAALPTAVAAVMFLL"
     gene            complement(12828..14114)
                     /locus_tag="PKV56_05845"
     CDS             complement(12828..14114)
                     /locus_tag="PKV56_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOX67370.1"
                     /translation="MGASPVTGLILTGGGARAAYQVGVLKALMRIRRDCCGIGRNPFD
                     VIAGTSAGAINAAALACRSDDIDGAVGRLVDTWAGFHADQVYLADSLGVIRSGARWLT
                     MLSLGWVLARWRRARPKSMLDNSPLRELLADLVPVHRLNDMMRGGHLKALAVSASGYG
                     SGLHATFYDTLADTAPWNRSQRVAVRQSITIDHLLASSAIPFVFPAIELGLDGRREYF
                     GDGSMRQSAPISPAVHLGAERVLVIGAGRMHEPALPIEGRVASHEYPNLAQIAGHALS
                     SIFLDALAVDVERLQRINRTLSLMTPEARINSGLKAIDVLVIAPSQRLDDIAARHLGS
                     LPQAVRALLRGVGVSGQGKQARGAALASYLLFEAPYTQELMALGESDTLIRRDEVASF
                     FGWDRRHRQRGEDDPRAVFSRAEDIDPAFATADSRF"
     gene            complement(14343..15086)
                     /locus_tag="PKV56_05850"
     CDS             complement(14343..15086)
                     /locus_tag="PKV56_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOX67371.1"
                     /translation="MATALKTRPNSSAVAAKPKRSASRAAPKGPASASLTLSSKNYSS
                     WSLRGWLMTRLAGLDFDEVIVSPDDASARKEILLLSPSILVPCLTHAGIKVWDTLAIG
                     EYLAEIKPKAGLLPADIAARAHCRAICGEMHSGFSSLRSALPMNLKGDFPGYKVWARA
                     EGDIERITAIWSECIAQYGGPFLFGEHPCMADAMYAPVVTRFRTYHVKLDRTSAHYCD
                     TVMAMPEMKEWIAAAKREHEDIEELDMEF"
     gene            15258..16505
                     /gene="phaZ"
                     /locus_tag="PKV56_05855"
     CDS             15258..16505
                     /gene="phaZ"
                     /locus_tag="PKV56_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOX67372.1"
                     /translation="MMYEAYQAQSDLLWPLRTFTKAALPMLKDHTYGWSRLTPNRKLA
                     AAWEVFGLSEITHQRRPFAIDEVAVTGVDEPVAITEEITHVASFATLLHFRKARLNDN
                     RHPKVLIVAPMSGHFATLLRETVRTMLADHDVYITDWHNARDVPLEHGKFGLDEYTEH
                     IIDFLAIMGPGSHLMAICQPCVSALAATAVMAEDHHPATPASLTLMAGPIDCRISPTA
                     VNQLAVTKPIEWFESNLIGRVPMRYPGAGRRVYPGFVQLTAFMSMNKDRHINSFKELY
                     QLLEKQEAEGLEKAEATRTFYEEYFAVADLPAEFYLETVKAVFQEYALPKGELTYRDR
                     IVKPSAIRRTALLTVEGERDDICAVGQTLAAHDLCTGLREYLKTHYVQAGVGHYGVFS
                     GRRWNQNIYPVVRESIHTAAASL"
     gene            complement(16509..18536)
                     /locus_tag="PKV56_05860"
     CDS             complement(16509..18536)
                     /locus_tag="PKV56_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX67373.1"
                     /translation="MNTAAASLAKSSIPDRSAQRRPAPKEGIFEFFRHQGVWSPGVKL
                     FRTLQFKTKALWVSIAFLIPMLGMFYLNWKTESSLIEATQVEQQGAEYVRPVLEWVKI
                     AQDRRTAAVLGEGSLDDLQQKAAAVMSRIEAQQSEKGEAFGTQKAFETFKQLQADLRA
                     KPVGATPDETYKDHSEAIAAALELSTEVVNSSGLILDPTAESYHLINKAMVWGPKQTE
                     FTSQLTTLGALSLKSNELGSERRQWIAITYSTQRFLDSFDEASFQNGLAGKPEAEKQA
                     YGMEHNDDMFDAFLEGLKTQVLGDKLQGTPQALLALGQTVLGTQGTMNAKLFDRVEAV
                     LRERVEGSTRVLVIESVVVFGFVLLGGYLFYSFYLVTQGGIRETQRHLEAMTAGDLTT
                     TPKPWGRDEAAHLMFSLADMQVSLRKIVTQVRGASESIVHASTEIAAASIDLSQRTEE
                     SAANLEESASSMEEISSTVRLTADHTAEAAQLATSNVTVANHGGTVIGEMVNTMQDIQ
                     ASSRKIGEIIGTIDGIAFQTNILALNASVEAARAGEQGRGFAVVASEVRSLAQRSAQA
                     AKEIKTLIEASVAKVESGTKVVRGAGDTMHEIVGNAQRMSSLLGEISTAAREQSAGIL
                     QVGASVQELDRTTQQNSALVEESAAAAAALKDQAIGLASEVSKFKLPGNAY"
     gene            complement(18669..19775)
                     /gene="aroC"
                     /locus_tag="PKV56_05865"
     CDS             complement(18669..19775)
                     /gene="aroC"
                     /locus_tag="PKV56_05865"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOX67374.1"
                     /translation="MSGSTLGHLFCVTNFGESHGPAIGCVIDGCPPGMVLSEADIQPE
                     LDRRRPGTSRHVTQRNEPDAVEILSGVYEGRTTGTPICLLIRNTDQRSKDYGNILQTF
                     RPGHADYTYWQKYGIRDPRGGGRSSARMTAPMVGAGAVAKKWLREHHGITFSGHMSQL
                     GDIAIPFESLDHVPNNPFFAANASDIARLEDYMDELRKAGDSCGARIDVAAHGVPVGW
                     GEPLFDKLDADIAHAMMGINAVKGVEIGAGFASVTQRGTTHGDEMTPTGFLGNNAGGV
                     LGGISTGQDVTVSIAIKPTSSIRSPRQSIDLQGQPVTVETFGRHDPCVGIRATPIAES
                     MLALVLMDHALRHRAQCGDVQVAMARIAAQQPGV"
     gene            complement(19918..20403)
                     /locus_tag="PKV56_05870"
     CDS             complement(19918..20403)
                     /locus_tag="PKV56_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOX67375.1"
                     /translation="MKVSQILTAKKGGTLFTVSSDIALSDAIKTMAEKDIGSLVVMDH
                     GDLVGMLTFREVIQAVVRNGGMVGSLIVRTVMDDSPLTCTPDTDMDEVRRMMLGRHAR
                     YLPVMTQRTLMGVISFYDVAKAVVDTQDFENRMLKAYIRDWPAEDTAPAAAEAGGSPA
                     T"
     gene            20730..22268
                     /locus_tag="PKV56_05875"
     CDS             20730..22268
                     /locus_tag="PKV56_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX67376.1"
                     /translation="MKLKSLNWSIFLLGAIGVLASLLVAAEGGWMQSRMGRNSTEVFV
                     AKDVVADILPPPLYLIELRLVLSQGVEGSMDAAETRKQFDRLVTEYGQRVDYWRKTPP
                     LGLEGALLGRQHEAGQKFIAAARAQVVDPLLAGDAAAARKGLADVHAVYLEHRAGVDE
                     TVALGSKLAETSMAQFEATRSLSTVVAIAISIGAIVLVLLFYRIVLRSIQQPLESCTR
                     LASRIAAGDLAASEPVDADRSDTIGALQKAHHEMQTRLAQVVSHVRQNSESVAAASSQ
                     IAQGNQDLSQRTEEQASSLQQTTASMADLNTRIQQNSDSARQANQLAMSASTVAVQGG
                     EVVGQVVTTMKSINDSSQRISDIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAVV
                     ASEVRSLAGRSGAAAREIKDLINASVARVDEGTALVDQAGATMQEVVNSIRRVTDIMG
                     EINAASVEQSRGAAQVGQAVSLIDQATQQNAALVEESAAAAESLKDQARQLVQSVAVF
                     RLAH"
     gene            complement(22315..23622)
                     /locus_tag="PKV56_05880"
     CDS             complement(22315..23622)
                     /locus_tag="PKV56_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HOX67377.1"
                     /translation="MESSPKLKATVADTVRQQVALWLVTLQTWPWLDTVHTLRQRFRE
                     DRLGLTASSLAFTTLISLVPLLTVMLAVFSAFPMFSSFQGSLEKYFLQALVPDAIAKP
                     VLGALTQFAGKAKRLGAVGLVVLVVTALALMLTIDRTLNSIWRVRDSRPIAQRVLVYW
                     AAATLGPLLLGFSLTFTSYAISASRGFVGGLPGGVSAMLQIVEFSLQALGMSALFYYI
                     PNTHVFWRHALAGGLFVAVGFELAKRALGWYLIQTPAYSTIYGAFATVPIFLIWIYLG
                     WVIVLLGAVIAAYAPTIQHRVVQRSHLPGYRFELAVSVLRGLARARQSDDRGLSVHQI
                     ANALNTDPLQVEPILDALVHIDWVGRLDEPGGARHVLLCEPAETAAQPLLASTLLDPS
                     EPLVAFWRHARFDEMTVQDLIAEPQAPQGPDAAAESGSPAHAA"
     gene            23733..24116
                     /locus_tag="PKV56_05885"
     CDS             23733..24116
                     /locus_tag="PKV56_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HOX67378.1"
                     /translation="MPPVVAWSRAVAVGSLLGLIVLGLAWELVLAPTGNRTLALKVLP
                     LAIPLTGLLKNRMYTYRWVSLVIWLYFIEGVIRASGDRGLSAWLAGGEVLLCVVLFAA
                     CAVHVRWRLYRAHALAAVEPSAPTA"
     gene            24151..25551
                     /locus_tag="PKV56_05890"
                     /pseudo
     CDS             24151..25551
                     /locus_tag="PKV56_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(25568..26080)
                     /locus_tag="PKV56_05895"
     CDS             complement(25568..26080)
                     /locus_tag="PKV56_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOX67379.1"
                     /translation="MKVLNLRCPSDHRFEGWFASEDDYRSQTERALIECPMCGVTTVQ
                     RLPSAPHVLTSTSRAEAHTQPVKPRQAQPRPGSVTESAAAGTPTAEAQPDQFMLQSAW
                     LRAVQHVIKHTDDVGTQFAEEARRMHYGEADERPIRGQATSDEARALHDEGIEVMALP
                     IPSAIKGTVQ"
     gene            complement(26150..26770)
                     /locus_tag="PKV56_05900"
     CDS             complement(26150..26770)
                     /locus_tag="PKV56_05900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOX67380.1"
                     /translation="MSEPDAHLREECIESEQVYLGHFLDVRRDVVRLPNGATASREYI
                     VHPGAVMIVPVLDDGRLVIERQFRYPVAQVMLEFPAGKLEAGEAPIVCAVRELAEETG
                     YRATEWARAGLTHNAIAYSTEGIEIWFARGLTAGATQLDEGEFLDVATATLEELEEAA
                     RLGTLTDVKTLVGLLWLRHWREGRWGLTWMSQEAAAAISVDASRSR"
     gene            complement(26781..28265)
                     /gene="nuoN"
                     /locus_tag="PKV56_05905"
     CDS             complement(26781..28265)
                     /gene="nuoN"
                     /locus_tag="PKV56_05905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOX67381.1"
                     /translation="MMNDMNWLAVYPEIFLLLATCVVALVDLWVTDPKRVFTYRLTQG
                     SLAIVALMFLYQYDAGLSVYAMQQMVVADPMGNVLAFFATVAVMISLAYARPYADGRE
                     MLKGELFTLSMFSLLGICVMVSANNFLVVYLGLELMSLSLYALTAMRRDSLNATEAAM
                     KYFVLGALASGFLLYGMSMMYGATGSLDIGEVFKAIGTGQINKAVLIFGVVFIVAGLA
                     FKLGAVPFHMWVPDVYQGAPTAITLLIGGAPKLAAFAITIRLLVEGMSGVAEHWQQML
                     IVLAVGSMAIGNLAAIAQRNLKRMLAYSTIANMGFMLLGLTPTVIGTNTLSAANGYSS
                     AMFYMVTYVLTTLGTFGVIMLLSRQGFESENIDDLKGLSKRSPWYAGVMAIFMFSLAG
                     VPPTVGFYAKFSVLQAIVSTNVYGYIVLAVAAVVFSLIGAFYYLRLVKVMYFDEPAET
                     APIVASGEVRALLSLNGAAVLLFGLLPGGLMALCVNAIGKALST"
     gene            complement(28262..>29090)
                     /locus_tag="PKV56_05910"
     CDS             complement(28262..>29090)
                     /locus_tag="PKV56_05910"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOX67382.1"
                     /translation="FAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGGYGFLRF
                     SLPITPDASHEWAWFIITISLIAVIYIGLVALVQQDMKKLVAYSSIAHMGFVTLGFFL
                     FNEIGVSGALVQMISHGFVSGAMFLCIGVLYDRVHSREIASYGGVVNTMPTFTAFAVF
                     FAMANCGLPATAGFVGEWMVILAAVQVNFWLGALAATALVFGAAYTLWMVKRVYFGPI
                     GNHHVRELIDLNSREMLIMTVLAVAVLWMGLYPKPFTDVMHVSVTELLKHVAVSKLN"
BASE COUNT         5126 a         9711 c         9643 g         4610 t
ORIGIN      
        1 tgagtaacgg ctggccccgt cctgccgaat gccttcgagc acgcccagac ctgtcctgtc
       61 agcgagctgc aggctcagcg tcatcgcgtc gtagaccgtg gtgcctgcct tgctgagggc
      121 ctgctcaccg gtggccgcac agtccacgtc gaagccgatc tcggaaagcg actgcaacgc
      181 cagcgccgag gcgggcccgt gctcctcgat caacagcatc cgattcctgg ccgccgcctt
      241 gccgagactg gcgccctggg cgtccgtgcc gtggacacgg ttcagaagca catgaaacac
      301 gctgcccacc ccgacggtgc tgcgcacccc gacgctgcca ccctgcgcct cgaccagccg
      361 acgcgtcagc gcgagcccca gccctgtgcc ctggtgctgc ttgctgtagc cggcgtcgag
      421 ttgctggaac tccacgaaca gccgcgacag gtctgtcggc gagatgccga tgccggtgtc
      481 ctcgacctcg atgcggaaat ggctcgctcc gtgcgccatc gcgcgcacgg tcacgcggcc
      541 gccctgcggg gtgaacttga tcgcattcga caggtagttg tagagcacct gcttgagacg
      601 tgccatgtcg agcacgaggt ccgtcagacc gggctcgacc tggacgtcga tctcgatctg
      661 cttgcgctgc gtggtggctt gcagcacatc ggacacctcc ttgagcagcg caggaagttg
      721 aaccggttcg gggaagaatt cgaacttgcc ggattcgacc ttcgacagat cgagcacatc
      781 gttgatcagt tgcagcagat gccgcccgct ggtcgagatg tggcccagga acttcgcgtg
      841 cttcggagag cccggcggca cggttcccga ctgcagcagg tcggcaaagc cgatcaccgc
      901 gttgagcggc gtgcgcagtt cgtgcgacat gttggccagg aactggctct tgagtcggtt
      961 cgccgcctgg atctgcctgt tctccacttc cagacgctgt gccgtcagcc ggacatcctc
     1021 ggcgagccgc tgctgggtga tctcggtgac gatgccgagc atcctgccgg catgcccctc
     1081 ctgaagttgc accttgccgt gggcactgat ccagtgaacg ctgccgtcgg gccagatcac
     1141 ccggcactgg acatgccagg gttgtgcgct ggtgagcgcc acgtgcaggc tgtgcgcgac
     1201 ctccgagcgc tcatcggggt gcacgtgctc gatgaaggtg tcgaaggtcc attcgggctg
     1261 catctcgtcg taaccgaagc agcggtcgtg tcgcagcgaa cgctgcatga cgcctgtcgc
     1321 caggtccatg tcccagtccc cgatggccgc cgattcgagc gtgaaccgca ggcgggtctc
     1381 gctgtcacgc aaggcggcct ctgcgcgtcg ctcgctggtc acgtcgcggg cgatcttcgc
     1441 ggccccgacg atgcgtccct cgccgtcacg gatcggcgaa accgtgaccg acacgtcgat
     1501 ccgccggccg ttcttcgcga ggcgcacggt gtcgaaccgg ggcacgcgtc caccgctgga
     1561 aatgttcgcg acgatgcgca actcctcctc ggcccgctcc ggaggaatca gcatcgtgac
     1621 cgactggccg atcgcttcgc cagcgctgta gccgaacagc gcctcggcac cggcattcca
     1681 gtcggtgatg cggccgtcga gcgtcttgcc gacgatcgcg tcgctggaag actccacgat
     1741 cgcggcaagc cggctgggtt cggcctcggc gcgaatcacg cctgtccggt cccggatcac
     1801 catggccatg ccgagcacgg tgccgtcgtt tgcccgcgtc aagcctgcct gcagctcaac
     1861 gggcgcccgc actccatcgc gagacaggac gatgaaacga tcgacgacat cgatcgtgat
     1921 gccgcgctcg atcatcgcgt cgaccggatt gcgtgacagg tcggaaagcg aactttcatg
     1981 acgctggaga acctccccga ccggccgtcc cctcgcgtcc gcctggaccc agcccgtcaa
     2041 ccgctccgcc acggcgttca tgctcgtgac ctgcccttcg cgatcggtcg cgatgaagcc
     2101 cgcgctgatg ctcgcgagcg tcaccgacag gttgtcctgg tgctgcacca gctgttcttc
     2161 gttgctgcgg cgctggcgga cttcggcttc gagttcaccg atcagcgact ggagccgctc
     2221 gaccgacgcc gtccgcagca tcttcgggat cagtggccac agcgccatgg cactgacgat
     2281 ggaaagactt tcagaggccg ccccgtgcag cccctgcagg ccgatcggcc agcagatgct
     2341 ccagtggtcc atcgcgcgcg agacgccgag gaacaagccg atggctccga tcagccaagg
     2401 cagccggccg agggaagcgg cacgccacct ccggcccagg aacgaagtga ggaaattcat
     2461 ggctcatcat ccggtaggga cagtacggaa aacggcgcat gccgatctcc tgtcaagatg
     2521 ccgagcccgc atccacagtg ggcaacccac ggtagcagaa ggaaaaaaga gccgcatttc
     2581 gatggcccta catcgcgcgc ggacgatgac gcccatgcgg atcgacgccg gtcgcgggcc
     2641 ggctccagaa tctcgggatg accacgatcc ccgaacccgc cagcgagcac cccgaaccgg
     2701 tgcgcatcca tatccaggag gtctgcgtgc gcgacggctt ccagatcgag ccggtcttcg
     2761 tgcccaccga gcgcaaggtc gcgctgatca acgcgctcgg ccgaaccggc ctgtcgaaga
     2821 tcgaggtcac gtcgttctcg tcgcccaagg ccattccggc gctggccgac gccgaggagg
     2881 tcatgcggcg catcgaccgc atcccgggcg tcgaatatgc cgcgctggtg ccgaatgtcc
     2941 gcggcgcaca gcgcgcgctg gcgtgccagg tcgacgaact gaatctggtg atgtcggcca
     3001 gcgcttccca caaccaggcc aacctgcgca tgagcatgga gcagtcgctc gcccagttcg
     3061 ccgacatcgt ggccgcggtc gacggtcagg cgcgcatcaa cgcttcgctg tcgactgcat
     3121 tcggatgccc gttcgatggc gaggtgccgg cggcgcgcgt gctggagctg gtgcagcgct
     3181 tcgtggatct tggtatcacc cgcgtggcgc tgtgcgacac caccggcatg gccaatccgg
     3241 cccaggtgcg acgactgttc agcgcagtga tcgaacgctg gcctgcggtc acgttcaccg
     3301 cgcacttcca cgacacccgc gcgatgggac tggccaatgc actggcagcg ctcgaagcgg
     3361 gtgtgcgcca tttcgacgcc tcgctcggtg gcctgggcgg ctgcccctac gcgccgggtg
     3421 ccagcggcaa tgtctgcacc gaggacatgg tgcacatgtt cgaggccatg ggctgtgaca
     3481 ccggggtcgc tctggagcca ctgctcacgc tggcgagaag cctgcccgac atcgtcggcc
     3541 acccggtgcc gggtcaggtg atgaaggccg gaccggccac gcgacgctac ccgctcggca
     3601 gcccgggttc agcccagccg gcctaggctg cagcgcggcg cgccacccag taggtcgcgc
     3661 cttcggcacc gtagcgctct gcgtgcggaa cgtcggcttc gagtgcgaac gtgtcaccgg
     3721 cggtcaggtg gcgtgacgct tcgcccacgc tgagccacat ctcgccgcgg accacgaccg
     3781 cctgcagcgc gaacggatgc acgtgtgtct cgagcaccgt caatggcggc caggttcgct
     3841 cgagcacctc gtcgaagccc tgtgcgcggg acgcagcctc gaactcttga aatgtcgggt
     3901 tcatcggtgt ggcggcggat tgaaggaggg ccggcaggtg cctgcatggc accagccgcc
     3961 tcttccatta tccgtacgcg ccccggcagt gcctggacgc cggctgcccg ctcaagcggc
     4021 ggcgcccggc agcacgtcgg ccagcttcaa tcgctgctgc tcgcgcaggc cttgcatgga
     4081 catgtcgatc accagcgctc cgctggcctt gccgcgcgac ttgtagacgc cgcagcccag
     4141 caccagatca ggcgtgacgg cctcgacata cgagctcttg aggtcggtgg ccttggtctg
     4201 cgggttcgcg aactggtagt cgacccagcc gccgccatgc gccgcctggt cgaaggcatc
     4261 acgaacgagc ttgtcaccgt cgacgcccgg cacctcgcgc accgacgtgc cgaccttgtc
     4321 ggccttgccc gcgaaagccc ggtagatgcc accgcgatcg aacgcgaaga cgtacatgtc
     4381 gcgatcgctg agcccgttgg ctgcatcggt gatctgctcg agggccgagt gaccgttcct
     4441 gcggtacatc tcgacggcct ggcgaaccag cgccagcgcc tcgtcggcgc tgccctgacg
     4501 cagcttgaag ttcgacaccg cggaactcag gcgctcggcc cgaccgctga gctgcgagga
     4561 actgttcatc gccgactcga ccatctgcgc attgcgctgg gtgatctcgt cgatgtgctg
     4621 caccgcctgc gcgatctggt cgaggccgct gctctgctcg acggtgctgg tcttgatctc
     4681 gtggacccgg ctggccacct ctcgaatgcc ctggacgatc tccgcgaagg tctggctcgc
     4741 gccaccgatc tgcgccacgc cggtgttcac gtgccccgcc gacgcggcga tcagcgactt
     4801 gatctcgcgt gcggattcgg aactgcgttg cgccagcgac cggacctcgg ctgcgacgac
     4861 ggcgaagccc cgaccctgct cgcccgcgcg cgccgcctcg acggcggcat tgagcgccag
     4921 gatgttggtc tggaaagaga tgccttcgat caccccgacg atttcctgca cccggctcga
     4981 actcgtctgg atgtcctgca tcgcgacgac tgccgactgg atggcactgc cgccgttctc
     5041 ggcgatcgtc tggacccgag ccgccatgct gcttgcagcc tcggcgccct gggcgttctt
     5101 gcgcaccgcc ccgctgagtt cctgcacgct ggccgtcgtc tgctccaggc tcgaagcctg
     5161 ggcctcggtg cgctccgaca gggccttggt gtccgccgcg aggctgagcc cggcctgcgc
     5221 gaccatcgat gaattgcttc gcacgtcggc gaccatctcg gagatgcgac cggtcatgtc
     5281 gtcgagcgaa tggcccacca ccgaaagctc gtcggtgcca cgcagacgga tgtgcgtcgc
     5341 gaagtcgccc gccgccagtt gcgtcaccga cccctgcagg acacggatgg cgccgaacga
     5401 ggtgcggaag aacacgatcg ccaggtaggc cgcgccgagc accgtgcaca gtcccacggc
     5461 cagttgcacg aaccacagcg tctggagacg cgcggcgcgc tcgtcgagca ggaactggag
     5521 ccggtccgtc gcgaccttgc cgatctgaag tgcctggccg atggctgccg tgccggcatc
     5581 gaagtacgag gcaggatcac cgacgagctt gggcgccgtg aaggtcgcct cggcttgctg
     5641 cgcgtagtcg cgtgcggctg ccagggcttc tgacaggccc ttcggtggcg cctcgccggc
     5701 gcgcctgagg gcaccgacga tgtcctcggc ggacaccagc gcgtgattca gcaggcgagc
     5761 ctgggcgatg atttccgcgc gatcgatggc gctggcctcg ccggtgcgca gcaaggccgc
     5821 accgcgacca cgcatctgcc ccagcgcctc tgtccacacc gccagcggct cgaccgagag
     5881 atgcatcagg tgaaacggcg cgccttcggg atcgagcagc agcccgctgg cctctgcggc
     5941 ccgcgacacg aatcggcgca tcgcctcgac ctgctgggaa tgcagcgcga agctttccga
     6001 ggcctgcacc gggatgtcgc cttcggccag acgctcgagc gagcgccgga tcggctgcca
     6061 gtccggaccg agatcgaatt ctgggtgctg gatgaccagc gactgcagtg caccgatcgc
     6121 cgacttcatc gcggcccggt tctgtgccag atcgccggcg actgcggtgt cacccgccag
     6181 cgcccggttg acgagaccgc ggtgcttctg cgtctgggcg atcacgtcga ggctcaggcc
     6241 gatcagcgga ttgccgcgcg cttcctgccg tgtcgattcg agggaagcgt gggacgccag
     6301 caaggcggtg acggtcagcc aggtcagcgg aaccatcagg acgaccgaga tcagcgtgat
     6361 cttggccgga aatttcattg cccgcatcag cctcatgccg ggccagaaaa acaggcggcc
     6421 cagtcccgta tccatcagat tcatgctcag ctccagttgg cgggcccccc ttcactggaa
     6481 aggcgagccg gttctctggg catatcggca ctctgcctgc gcgcctttaa tttgaacaat
     6541 tcatacaaat attccagaaa gcaactgatc ctcgagccat tgccgcatga agccgccgac
     6601 cagcatctcc atgtccggca cgccgctcgc agcaacatct gaacggttgc cgatgcattc
     6661 ctccgcctgt gcgcaggcct cggcgaaggt cgacccagcg agcatcgcgc gcagcaaggc
     6721 ggcctccgtg gaatgcagtg accggaagtg cggctgcagg cccttgcgcc agaccagcca
     6781 gtccactcca ttcacagagg cctgggtttc gggcggatcc tggtcgtcgt cgatggcctg
     6841 ccagaccgcc accgtgttgt gacgaaacac ccgcagttgc acggtcggca ccggcaccag
     6901 ccgcagcgtc gcccaggccg attcggccag gtcggcgaac gtaccagcat cgagcacccc
     6961 ggagtcgggc ccgtcgaacg cgctgcgcag agcccagtcg agccgggcca gctcggacca
     7021 cgcgctcgat gccggcaact cggcatcgcc ctgcgccagg aggtgctccg cgaattgctc
     7081 gccgtaccag cgcaggctcc gtgtcgtcgg tggatgtgca gcgatgtaat cgagtgcggc
     7141 ggcctcgaaa gcggcttcgc ccatgaccag gcacgtcttc tcgtaggaat cgatcagcgt
     7201 ttcaagcagc cgttgccggt aggcgttggc atagatgcgc aagcccagct gccggcgtgc
     7261 ggcatcgccg gcgatcagcc gcgaggcatc gccggctccc agcatgtgct gctggaactg
     7321 atgctgcgtg gacagcagcg acgcgctcaa gcgcagctcc tgacgggctc gcgccgggtc
     7381 gtctcgagcg cgtcggcagc gatctccctg gcccggtcga gttcgcagac cagttcggcc
     7441 agcggcggga tgcgatcgtc gcgctcgatc atcgtcgcca ccggaccaag ccgcgccagc
     7501 gcctcggcat agagcgccca caccggatcg gccaccggat gatcgtgcgt gtcgatgagg
     7561 tgatcgccgt gatcggtgtg gcctgccaga tggatctgct gcacccggtc ggccggcacg
     7621 ccacgcagga acgcccgggc gtcgaacccg tggttgacgc tgctcacgtg gatgttgttc
     7681 acgtcgagca gcaggcggca accggtgcgc tggcagacct cggtgatgaa ctcccattcg
     7741 gtcatttccg acgattcgaa gctgacgtag ctggagacgt tctcgagcac cagcggacgc
     7801 ttcagcacct cctgcgcgat gtgcacgttg cgcaccacga catcgatcga cgcatcggtg
     7861 tacggcagcg gatacaggtc gtgcagcacg cggccatgca caccggtcca gcacaggtgg
     7921 tcggagatcc acagcggttc aacgcggcgc gccagcgcgg cgagtgcggc gaggtaggcg
     7981 cgatcgggtc catcggcggc accgatcgac aaggccacgc cgtgcatcac gaccggatag
     8041 tcggcccgga tggcgtcgag catgaccagc ggcttgccgc cgggcacgag gtagttgtcg
     8101 gacaggatct cgagccagtc gacccgctgg ggcgactcga ggaaatcgga gtaatgcggg
     8161 gtgcgcagcc ccaggccgaa accggggttc gcaagccggg gctgcgcgtc agacatggcc
     8221 tcaggcgttg atcacaggtc gccgatggtg ccgccggcct tgaggcattc ggctgctgcc
     8281 atgcccttga atccgtggcc gccgcaggcg ttctgcccct tgcagctgtg ctcggcggtc
     8341 ttgcagtccg agttgccctt gcagctgtgc acgccgtagc agtggacctt gtcgctggcg
     8401 gcgatggcct tgccggaact gcccttgggc gcttcggcgg cgaatgcggt ggcagacagc
     8461 gccagggcgg cggcggcgga agcgatctgg aaacctctgg aagcattgaa catgttgaac
     8521 tcctgcgtat gaagggtggg ttggcgcctg cagacactgc aggccttggg tgacgggggt
     8581 gaatcccatg caccttcttc tgtcggcgcg ggatcgaatc ccttacaccg ccgacaccac
     8641 cgatacgcag tgcagggcga atcagatgcg acgtgacgac gcaggccctg cggcactcgc
     8701 cgcatcgacc ggcggcgccc cgaccacgag cgcgcgcaac cggggccaga cgaagcgatc
     8761 caccgaccac cgacccggtc cccacagcaa cagcccgacc agcacgctgc cccagaagat
     8821 gtgctgctgc agcgccggga tcggcatgtc ctcgacgctg atgaccgcca cgatgttcag
     8881 gatgaagagc ccggcggcgg cgaaccgccc ggcgaatccg aacaggatca gcaccggcag
     8941 cgccagctcc gcgctggtgc cgagcgcagc ggccagttca ggactgagca acggcacgtg
     9001 gtactcgtcg gtgaacagcg cgacggtcgt ttcccagtcg gcgatcttgg tcaggccgga
     9061 gcggaagaac acaccggcga tgaaccagcg cgccgccagc tgggcagccg gttgcaacgc
     9121 ttcgaggcca cggatggcgg tttcaccgag cgtccacaga cgcgaccaga gtgaggcgga
     9181 atgaggttga ttcatcttgg gtctccaaca aggttccatt cagtcggagc agacgcggat
     9241 ttctgtcaac cagccctcgc gcagcgcggt ggccagccag gtgcccaggt cgaacgcgat
     9301 gtcgcggtcg gcatcggcgg ccgcatcgac ggcgccgagt gctgcactca gggagtcgcc
     9361 agcgagcagc cgttcggtcc acagcgcggt ggcggcatcg atgcggtgca cctgcgcacg
     9421 ccagccggat ccagcgcccg atcgcgccac cagcgcgatc tcgccaacac cctgcgccaa
     9481 cgcttcgctg acggcagcca gggctgattt cgtgtccgcc tggcgatggg catcgtggat
     9541 cagcaccacc ggccagcgag accgcagtac ggcggtgccc ggccgcagtt cgagatgcag
     9601 gtgccgggga tcggtgtcgc cgagacggga gatcgacccg gcatcgaaca ccgcatccgc
     9661 cgcgcgttcg cactgatgca cggcccagtc gagtcgggcg cagtcgccca ggtagggcca
     9721 ttcgagcagc tccggatgcg cttcgagccc ggcgcccagc gccccgcccc attcggccag
     9781 atcgccgctg gtcggcaggg cctgcatcca gtgctcgcgc gccagccgtc gaaaatcatc
     9841 ggagccgatc agcgcctgca ccgtggggca ggccccctcc agcgcgcgcg cggcatgggc
     9901 ccccgcgttg gcacggtagg ccgccaggcc gcgcgccacc ggaaccggtc cgtcgacgca
     9961 cggcagatcc cgcgcggcct gcggcgcatc ggcggtccaa agcgccgcga tcagcgcacg
    10021 ttgccgttcg gattccgcag acgttgcagc cgaagccgcg ggttcgtcgc cggcagtcat
    10081 cgccgcaccc gggccagtcg ttcggtggcc tgccgagcct gcccgagcag cacgtcgagc
    10141 gccggtacat cggtgtccca ttcgaccagc gtcggcaccg cgccgaagcg ctgcagggca
    10201 tgctcgtaga gcgcccacac agcgggccgc acgaggctgc cgtgatcgtc gatgacgatg
    10261 tccggcaact ggcagtagcc cgccagatgc acctcgccga cgatgccggg cggcagggca
    10321 tcgatgaagg cgcacccgtg cgcgagcgga tcggccacgc gctcgttcaa cgcgttgacc
    10381 atcaggttgt tgacgtcgag cagcaggccg caacccgtgc gcctgcaaag cgcagcgaga
    10441 aactcgggtt cggtcagcgt gttgtcggcg aaggtcaggt acgccgacag gttctcgacc
    10501 agcatctgcc gttgcagccg gtcctgcacg cggctcacgt tgtcacagag gatcgacagc
    10561 gacgctggcg tgaacgcgat cggcagcagg tcattggcat gcaccaccgg gccgccgggc
    10621 tcgcgggggg cgcgcgcgaa gctggcgtgg tcggagacca gcgcaggttc gattcggcgc
    10681 accagccggt ccagccgatc cagatggccg gcatccacgc ccgccgccga gcccagcgac
    10741 aggccgacgc catgcaggct gaccggataa cgagagcgcg cctcgtccag caccgccagg
    10801 gcagcgccgc catcggcgta gaagttctcg gagtggacct ccacgaaacc gatggcgggc
    10861 gggtcctcga acacctgtgc gtaatgcggc tggcgcagcc cgatccccgc gagttcgcgg
    10921 cagaccgggg gcaaggccga caacacggga ccggaccgac gcctgagaaa gcgtccgctc
    10981 ttacttcttc atggccttct tggcttcgtc ggccgacttg ccgcccagcg aagcgcaggt
    11041 gcccttggcg acgtacttcc actcatccgc agcgttgtcg gccttggcct gaccggcgca
    11101 cgagtgcgtg cccgacaggt tggcgcaatc gttggcgccg gcctttgcga tgccgaagca
    11161 cttctccttg gccggtgcgt cggcggcgga agcagtcgac agcatgccga gcgcaagcac
    11221 ggaagccagg gcggaagaag cgatgaggcg atggttcatg gaaatctcct gagttgagtg
    11281 gtgtgaatcg actgcgctgg ccggtgtcat ccgacgcttg cccaaatgag tcgcgcgaca
    11341 gggacgttgc ttacaagcat ctggcgacgg gcggattgtg agggggtcgc atgacccgac
    11401 tacgggcaaa ggcgcccaag ctgcacggcc tccaggcgcc gcgcaggtca gagcaggaac
    11461 atcaccgcgg cgaccgcggt cgggagcgca gcgcccagca gcagcccgcc ggcgcccacc
    11521 gactgatcct cgaatccgtg cttcagcagc gtcactccgg ccgggttcgg cgcgttcgcg
    11581 atcaccgtca ggccgccccc tgcgacggca ccggacacca gcatgtactt ggcctgctcg
    11641 gacatgccgg aaatcagcga cccgaggtac gtgagcgcgg cgttgtcggt gatcgccgtc
    11701 agccccagcg cgccgaagaa cagggccgtc ggctccaggc tggagacgat cggctgcagc
    11761 caccaccgct gcatgccacc gagcacgacc agtccggcca ggaagaaccc gaccagcaac
    11821 gcctccttga ggatcagcgg gctctgatgg cgggcatagg cctgcgtgaa tcccaggaac
    11881 aacatgaaca cgccgaggaa aatcaccggg tggtgggcaa aggtgaccac catcgacagc
    11941 gcgcccagat ggatcagggt gaccaccagc gggatgcgct ccccggattc gtcggacgtc
    12001 ggcttcgcgg gttcggtcag gtgccggctc agcagcgcgg tgaccgcggt cgcgttgatg
    12061 acgacggcga tcgccgcctt ccagccgaag tggctggcca tgtaggcact gtcccattgc
    12121 caggtggcgg ccaccatcag cactggtggc gcagcgaacg aggtcagcgt gcctccgatc
    12181 gacacgttga cgaacagcac gccgagtgcg ccgtacttca gcccctcggg aacatcgccc
    12241 cgaaacacca gtggcgccag catcagggcc gccagcgtca tcgccgcggg ctcggtgacc
    12301 agcgagccga tcagcggcac cgcggccagg cagagccaga cccgggcgac ggcggtgcga
    12361 atcggcagca gccgggcaac ggactcgatc agcgcgcgga tcgtctcgag cacaggccgc
    12421 gaagccgcca ccaccatgat gacgaagacg aacaacggtt cggtgtactg gcgcgattcg
    12481 gcgtagtcga tggccttctg cccgccctcc accgccgcca tgaacgcgat caggacgaac
    12541 gcccagaacc cgaacaccac ctcgatctcg cccagcaggt ggaagagccc ggcgttgcgc
    12601 gggctgcggt gcgccagccg ctcgaagaac ttgaccgaga acgtgtggag cagcgcgatc
    12661 gcgaagatca cggctgcgat gagttcgatg gtgctcatgg aatccggggc tggggaagcg
    12721 ccgatcgcgc ctgagctggg cctgcagcgc aggacaggat cgcggcaaga tcgatggaag
    12781 ttgcgagtat ccgcgacggc gtgcaggcgc cccgcagcgc aagcgcgcta gaaccgcgag
    12841 tcggccgtcg cgaaggcggg gtcgatgtcc tcggcgcgcg aaaagaccgc gcgcggatcg
    12901 tcctcgccgc gctgccggtg gcgccgatcc cagccgaaga agctcgcaac ctcgtctctg
    12961 cggatcagcg tgtcggactc gccgagcgcc atcagttcct gggtgtaggg tgcctcgaac
    13021 agcaggtagc tcgccagcgc ggcaccacgc gcctgcttgc cttggcccga cacgcccaca
    13081 ccgcggagca gcgcgcgcac cgcctgcggc aggctgccca ggtggcgggc cgcgatgtcg
    13141 tcgagccgct ggcttggcgc aatgaccagc acatcgatcg ccttcaggcc gctgttgatg
    13201 cgcgcttcag gggtcatcag tgacagcgtc cggttgatgc gctggagccg ttccacatcg
    13261 acggcgagcg cgtccaggaa gatgctggac agcgcatgac cggcgatctg cgccaggttc
    13321 gggtactcgt ggctggcgac gcgtccctcg atcggcaatg cgggctcgtg cattcgcccg
    13381 gccccgatga cgagcacccg ctcggcaccg aggtgcaccg caggagagat cggcgccgac
    13441 tgccgcatcg agccgtcgcc gaagtactcg cgcctgccgt cgaggccgag ctcgatggcc
    13501 gggaacacga acgggattgc cgacgaagcc agcaggtgat cgatggtgat cgattgccgc
    13561 accgccaccc gttgagagcg gttccacggc gcggtatccg ccagcgtgtc gtagaaggtc
    13621 gcatgcagcc ccgagccgta gcccgacgcg ctcacggcga gcgccttcag atggcctccc
    13681 cgcatcatgt cgttgaggcg atgcaccggc accagatcgg ccagcagttc gcgcagcggg
    13741 ctgttgtcga gcatcgactt cgggcgggca cgccgccaac gggccagcac ccaaccgagc
    13801 gacagcatcg tcagccagcg cgccccgctg cggatcacgc ccagcgagtc ggcgaggtac
    13861 acctgatcgg cgtggaagcc cgcccatgta tcgaccagcc gaccgaccgc gccgtcgatg
    13921 tcgtccgagc ggcaggccag cgccgcggca ttgatggcgc cagcggaggt gccggcgatc
    13981 acgtcgaacg ggttgcgacc gatgccacag cagtcgcggc ggatgcgcat cagcgccttg
    14041 agcacgccga cctgatacgc ggcacgcgca cccccacccg tcaggatcag gccggtgacg
    14101 ggggatgcgc ccatggcgtc agtctgtcac gccggcgcaa gccacggcag cggatcaatg
    14161 cggtgttagc ggcgcattcc ctcgcggctc aatgtgggag ttccacgggc acgaccgccg
    14221 cgttctccag gggtcgatca agacacccgc ggtgcagttc gaggggaacg gcagactgcc
    14281 ctgcaacgaa ggcaccgaaa tggtgcgctc agaaccctgc acaacggcga accaccgtcg
    14341 cctcagaact ccatgtcgag ttcctcgatg tcctcgtgtt cgcgcttcgc ggcggcgatc
    14401 cattccttca tctcgggcat cgccatcacc gtgtcgcaat agtgggccga cgtgcgatcg
    14461 agcttgacgt ggtaggtgcg gaaacgcgtc accaccggcg cgtacatcgc gtcggccatg
    14521 cagggatgct ccccgaagag gaacggcccg ccgtactgcg cgatgcactc gctccagatc
    14581 gcagtgatcc gctcgatgtc gccttcggcg cgcgcccaga ccttgtagcc cgggaagtcg
    14641 cccttcaggt tcatcggcaa ggccgagcgc aggctgctga agcctgaatg catttctccg
    14701 cagatggcgc ggcaatgcgc ccgcgcggcg atgtcggcag gcagcaggcc ggccttcggc
    14761 ttgatctcgg cgaggtactc gccgatggcc aacgtgtccc acaccttgat gccggcgtgg
    14821 gtcaggcagg gcaccaggat cgacggcgac agcagcagga tctccttgcg cgcgctggcg
    14881 tcgtcgggag agacgatcac ctcgtcgaaa tcgagtccgg cgagtctggt catcagccag
    14941 ccccgaagcg accaggacga gtagttcttg ctgctcagcg tgaggctggc cgaggccggc
    15001 cccttgggcg cagcacgcga tgcacttcgc ttgggcttcg ctgccacggc actgctgttc
    15061 ggacgggtct tcaatgcggt ggccatcagc tgagctccat tcgatcgtta tgcaccacaa
    15121 cgagtgcaag cgttgtgcca gtgccttcca tcacccggcg ggcggaacat ccgcggcatc
    15181 gcggtaatcg cccgacggac cgctgctggc gcgggagttg caagagcagc tgcatgccaa
    15241 gttcctggag agagcggatg atgtacgagg cctaccaggc gcagtccgac ctgctttggc
    15301 cgttgcgcac cttcaccaag gctgcgctcc cgatgctcaa ggaccacacc tacggctgga
    15361 gccgcctgac gcccaaccgg aagctcgctg ccgcctggga ggtgttcggg ctcagcgaga
    15421 tcacgcacca gcgccggccg ttcgcgatcg atgaggtcgc ggtgaccggt gtcgacgagc
    15481 cggtggcgat caccgaggag atcacgcacg tcgcgtcgtt cgcgacgctg ctgcatttca
    15541 ggaaggcccg cctcaacgac aacagacacc ccaaggtgct gatcgtggcg ccgatgtcgg
    15601 gccacttcgc gacgctgctg cgcgagacgg tgcgcaccat gctggcggac cacgacgtgt
    15661 acatcaccga ctggcacaac gcgcgcgacg tgccactcga gcacggcaag ttcgggctcg
    15721 acgagtacac cgagcacatc atcgacttcc tcgccatcat gggcccgggt tcacacctga
    15781 tggccatatg ccagccgtgc gtgtcggcgc tggccgccac ggcggtgatg gccgaagacc
    15841 atcaccccgc cacgcccgcc agcctcacgc tgatggccgg cccgatcgac tgccgcatca
    15901 gcccgaccgc ggtcaaccag ctcgcggtca ccaagccgat cgaatggttc gaatccaacc
    15961 tgatcggccg cgtcccgatg cgctatccgg gcgccgggcg gcgtgtctac ccgggcttcg
    16021 tgcagctgac cgcgttcatg agcatgaaca aggaccgcca catcaactcc ttcaaggagc
    16081 tgtaccagct gctcgagaag caggaggccg aaggcctcga aaaggccgag gccacgcgca
    16141 cgttctacga ggagtatttc gcggtggcag acctgccggc cgagttctat ctggaaaccg
    16201 tgaaggcggt cttccaggaa tacgccctgc cgaagggcga gctcacctac cgcgaccgca
    16261 tcgtcaagcc ctcagcgatc cggcgcacgg cactgctcac ggtcgagggc gagcgcgacg
    16321 acatctgcgc ggtgggccag acgctggcgg cgcacgatct gtgcaccggc ctgcgcgagt
    16381 acctgaagac gcactacgtg caggcgggcg tcggccacta cggtgtgttc agcggccgac
    16441 gctggaacca gaacatctac ccggtggtgc gcgagtcgat ccacaccgcc gcggcatcgc
    16501 tctgacgatc agtaagcgtt gcccggcagc ttgaacttgg acacttcgct ggccaggccg
    16561 atggcctgat ccttcagcgc ggcagctgct gccgcacttt cctcgaccag ggccgagttc
    16621 tgctgcgtcg tgcggtcgag ttcctgaacc gacgcgccga cctgcaggat gccggcgctc
    16681 tgctcgcggg ctgcggtgga gatctcgccc agcaatgaac tcatgcgctg ggcgttgccc
    16741 acgatctcgt gcatggtgtc gcccgcgccg cgcacgacct tggtgcccga ctcgaccttg
    16801 gccacgctgg cttcgatcag agtcttgatt tccttggccg cctgcgcgct gcgctgggcc
    16861 aggctgcgca cttcggacgc aaccaccgcg aagccgcggc cctgctcgcc ggcgcgcgcg
    16921 gcctcgaccg acgcgttcaa cgccaggatg ttggtctgga aggcgatgcc atcgatcgtg
    16981 ccgatgatct cgccgatctt gcgtgacgaa gcctggatgt cctgcatggt gttgaccatc
    17041 tcgccaatca ccgtgccgcc gtggttcgcg accgtcacgt tgctcgtcgc gagctgagcc
    17101 gcttcggcgg tgtggtcagc agtcaaccgc acggtagagg agatctcctc catcgacgaa
    17161 gcgctttcct cgagattggc ggccgattct tcggtgcgct gcgacaggtc gatcgaagcc
    17221 gcggcgattt cggtgctcgc atggacgatc gattccgacg cgccacgaac ctgcgtcacg
    17281 atcttgcgca gcgagacctg catgtcggcc agcgagaaca tcaggtgggc agcttcatcg
    17341 cggccccatg gcttcggcgt ggtcgtgagg tcgccggcgg tcatggcctc gaggtggcgt
    17401 tgcgtctcgc ggatgccgcc ctgcgtgacc aggtagaacg aatagaacag gtagccgccg
    17461 agaaggacaa agccgaacac gacaaccgac tcgatcacca atacgcgggt agagccttcg
    17521 acccgttcac gcagaaccgc ttccacacgg tcgaacagct tggcgttcat ggtcccctgg
    17581 gtgccgagca cggtctgccc cagcgcgagc agcgcttggg gcgtgccctg cagcttgtcg
    17641 ccgagaacct gcgtcttcag gccttccagg aacgcatcga acatgtcgtc gttgtgctcc
    17701 atgccgtaag cctgcttttc cgcctcgggc ttgccggcca ggccattctg aaaggacgcc
    17761 tcatcgaagc tgtccaggaa tcgctgggtc gaataggtga tggcaatcca ctggcgccgc
    17821 tctgaaccca gttcgttgga cttgagcgac agcgcgccga gcgtcgtcag ttgcgatgtg
    17881 aattcagtct gcttcggccc ccagaccatc gccttgttga tcaggtgata cgactcggcg
    17941 gtcgggtcca ggatcaggcc ggaagagttc acgacttcgg tggacagctc gagcgccgca
    18001 gcgatcgctt cgctgtggtc cttgtaggtc tcgtccggcg tggcgccgac gggtttggcg
    18061 cggaggtcgg cctgcagttg cttgaaggtc tcgaaggcct tctgcgtgcc gaacgcctcg
    18121 cccttctcgg actgctgggc ctcgatgcgg gacatgaccg ccgcggcctt ctgctgcagg
    18181 tcgtccagtg aaccctctcc cagcaccgcc gcggtccggc gatcctgagc gatcttgacc
    18241 cactcgagca cgggccggac gtactcggcg ccctgctgct cgacttgcgt cgcttcgatc
    18301 aggctcgatt ccgtcttcca gttcaggtag aacatcccga gcatcgggat caggaaggcg
    18361 atggagaccc agagcgcctt ggtcttgaat tgcagggtgc gaaagagctt cacaccgggc
    18421 gaccagacgc cctgatggcg gaagaattcg aatatcccct ccttgggcgc aggtcggcgc
    18481 tgggccgagc gatcagggat ggatgatttg gcgagactgg cagcagcagt attcatgggc
    18541 agttccgtaa agcggacagg tccaggacag tgggtgcgac ctcggatgtc gggccgctac
    18601 gcaccatttc ggacggtgtc aacgaaactt gaccagaacc tgaagaactt cttcagtccg
    18661 gcaccgcgct agacgcccgg ctgctgcgcc gcgatgcgtg ccatggccac ctggacatcg
    18721 ccgcactggg cgcggtgccg cagcgcgtga tccatcagca ccagcgccag catcgactcg
    18781 gcgatgggcg tcgcgcggat gccgacgcac ggatcgtggc gaccgaaggt ctccaccgtg
    18841 acgggctgac cctgcaggtc gatcgactgg cgcgggctgc ggatcgagct ggtgggcttg
    18901 atcgcgatgg acacggtgac gtcctgcccg gtcgagatgc ccccgagcac cccgccggcg
    18961 ttgttgccca ggaagcccgt cggcgtcatc tcgtcgccgt gggtggttcc gcgctgcgtg
    19021 acgctggcga agccggcgcc gatctcgacg cccttgacgg cattgatgcc catcatcgcg
    19081 tgggcgatgt cggcgtcgag cttgtcgaac agcggctcgc cccaccccac cggcacgccg
    19141 tgggctgcca cgtcgatgcg cgccccgcac gaatcgccgg ccttgcgcag ttcgtccatg
    19201 tagtcctcga gccgggcgat gtcgctggcg ttggcggcga agaacgggtt gttcggcacg
    19261 tggtcgagcg actcgaacgg gatcgcgatg tcgccgagct gcgacatgtg cccgctgaac
    19321 gtgatgccgt ggtgctcgcg cagccacttc ttcgcgacgg cgccggcgcc gaccatcggc
    19381 gccgtcatgc gggccgacga gcggccgcca ccgcgcggat cgcgaatgcc gtacttctgc
    19441 cagtaggtgt agtcggcatg gccggggcgg aacgtctgca ggatgttgcc gtagtccttg
    19501 ctgcgctggt cggtgttgcg gatcagcagg cagatcggcg tgccggtggt ccggccctcg
    19561 tacaccccgg acaggatctc gaccgcatcg ggctcgttgc gctgggtgac gtgacggctg
    19621 gtaccgggac gccggcggtc gagctcgggc tggatgtcgg cctcggaaag caccattccg
    19681 ggcgggcagc cgtcgatcac gcaaccgatg gccgggccgt gcgactcgcc gaagttggtg
    19741 acgcagaaca gatgaccgag ggtgctgccg gacatggcga cgggctttcg atgaaggcga
    19801 tgggagacgg cggagcgaca ggcccgccgg gcaaggacgc aagcatagcg ggcagaaaaa
    19861 caccgcctgg cggcggtgtc cggaccggcg gcgccacagg ccgcaggcag caacgcgtca
    19921 ggtcgcgggc gacccgcccg cctcggctgc cgcaggcgcc gtgtcttcgg ccggccagtc
    19981 gcggatgtag gccttgagca tgcggttctc gaagtcctgc gtgtcgacca cggccttggc
    20041 cacgtcgtag aacgagatca cgcccatcag cgtgcgctgc gtcatcaccg gcaggtagcg
    20101 ggcgtggcgg ccgagcatca tgcggcgcac ttcgtccatg tcggtgtcgg gggtgcaggt
    20161 cagcgggctg tcgtccatga ccgtgcgcac gatcagcgag ccgaccatgc cgccgttgcg
    20221 aacgacggcc tggatcactt cgcgaaaggt cagcatgccg acgaggtcgc cgtgatccat
    20281 gacgaccagc gagccgatgt ccttctcggc catcgtcttg atggcatccg acagcgcgat
    20341 gtcggaactc acggtgaaga gcgtgccgcc cttcttggcg gtcaatatct gggaaacctt
    20401 catgtcgttc tcctcgcggg agtcgtaaac gtcgtcaata taggcgcaaa taccccctgg
    20461 cgacaggctc cggtaccagg aacgcgcctg gaaatcgacc atgagcaagg tcccgcaagc
    20521 catcggttcg gccgctgaga gctgccagtc catgccctgc ccgctgcgac tcgaacccac
    20581 gcctctgctc gcaccgccag tcgcaagacg atgccaggca ccggcacccc gccttaagaa
    20641 aaatgaagac cactccgata agcggggcaa ttgcgccgct ttgcaagcgc gcgtccgcca
    20701 tgagcatggc ttacccggac tgtcggaaga tgaaactgaa atcgctgaac tggtcgatat
    20761 tcctgctggg cgccatcggt gtgctcgcgt cgctgctggt ggccgccgag ggcggctgga
    20821 tgcagtcccg gatgggacgc aactcgaccg aggtctttgt cgccaaggat gtggtggccg
    20881 acatcctgcc gccgccgctg tacctgatcg agttgcggct ggtcctgtcg cagggcgtcg
    20941 agggttcgat ggatgccgct gagaccagga agcagttcga caggctggtc accgagtacg
    21001 ggcaacgcgt cgactactgg cgcaagacgc ctccgctcgg actcgaaggt gcgttgctcg
    21061 ggcggcagca cgaagccgga cagaagttca tcgcggcagc gagggcgcaa gtcgtcgacc
    21121 cgttgctggc gggcgatgcg gctgcggcac gcaagggact ggcggatgtg catgcggtct
    21181 acctggagca ccgggccggt gtcgacgaga ccgtcgcgct cggcagcaag ttggccgaga
    21241 cgtcgatggc gcagttcgag gccacccgca gcctgtcgac cgtcgtcgcc atcgcgatct
    21301 cgatcggcgc catcgtcctg gtgctgctgt tctaccggat cgtcctgcgc agcatccagc
    21361 agccgctcga gagctgcacc cggctggcca gcaggatcgc agccggtgac ctggccgcga
    21421 gcgagccggt cgatgcggat cgcagcgaca cgatcggcgc cttgcagaag gcccaccacg
    21481 agatgcagac ccgactggct caggtggtga gccatgtacg gcagaactcg gaaagcgtgg
    21541 cggccgccag ctcgcagatc gcgcagggca accaggacct gagtcagcgc accgaggagc
    21601 aggccagttc gctgcagcag accaccgcgt cgatggcaga cctcaacacc cggatccagc
    21661 agaactccga cagcgcccgg caggcaaacc agcttgcgat gagcgcctcg acggtggccg
    21721 tccagggcgg cgaggtggtc ggccaggtgg tcaccacgat gaagagcatc aacgactcca
    21781 gccagcggat ctccgacatc atcggcgtga tcgacagcat cgccttccag accaacatcc
    21841 tggcgctgaa cgctgccgtc gaagccgcac gtgccggaga gcagggccgc ggattcgcgg
    21901 tcgtggccag cgaagtgcgc agcctcgcgg gccgcagcgg cgccgcggcc cgcgagatca
    21961 aggatctgat caacgccagc gtcgcgcgcg tcgatgaagg caccgcgctg gtcgaccagg
    22021 ccggcgcgac catgcaggag gtggtgaact cgatccggcg cgtgaccgac atcatgggcg
    22081 agatcaacgc agcctcggtc gaacagagcc gcggcgccgc ccaggtgggt caggcggtga
    22141 gcctgatcga tcaggccacg cagcagaacg ccgccctggt cgaggaaagc gctgcggccg
    22201 ccgaatcact gaaggaccag gcgcgccagc tcgttcaatc ggtggcggtg ttccggctcg
    22261 cccactgagc gggccaacgg gtcagtactg agccggtctg ccgcgacggc gagatcaggc
    22321 cgcgtgcgcc ggagaacctg actcggcggc tgcatcgggc ccctgcgggg cctgcggctc
    22381 ggcgatcagg tcttgcacag tcatctcgtc gaagcgtgcg tgacgccaga acgccacgag
    22441 cggctcggag gggtcgagca acgtcgatgc aagcagcggc tgagctgcgg tctcggcggg
    22501 ttcgcacaaa agcacgtggc gcgcaccacc gggctcgtcg aggcggccga cccagtcgat
    22561 gtgcaccagc gcgtcaagga tgggctcgac ctgcagcggg tcggtgttca gcgcgttggc
    22621 gatctggtgc acgctcaagc ccctgtcgtc cgactgccgg gctcgcgcca gaccgcgcag
    22681 aaccgagacc gccaattcga accggtagcc cggcaggtgg ctgcgctgca cgactcggtg
    22741 ctgaatggtg ggcgcatagg cagcgatcac cgcacccagc agcacgatga cccagcccag
    22801 gtagatccag atcaggaaga tgggcaccgt ggcgaaggcg ccgtagatcg tcgaataggc
    22861 aggggtctgg atcaggtacc agcccagagc ccgcttggcg agttcgaatc cgacggcgac
    22921 gaacagccca ccggccagcg cgtgccgcca gaacacgtgc gtgttgggga tgtaatagaa
    22981 gagcgcgctc atgccgagcg cctgcaggct gaactcgacg atctgcagca tcgcgctgac
    23041 cccgccgggc aggccgccga cgaacccgcg agatgcggaa atcgcatacg aggtgaacgt
    23101 gaggctgaag ccgagcagca acggaccgag cgtcgccgca gcccagtaca ccagcacgcg
    23161 ctgcgcgatc gggcgtgagt cgcgcacgcg ccagatcgag ttgagggtgc ggtcgatcgt
    23221 cagcatcagt gccagcgcgg tgaccaccag caccaccagc ccgacggcac ccaggcgctt
    23281 ggccttgcca gcaaactgcg tcagtgcccc gagcaccggc ttggcgatgg cgtcaggaac
    23341 cagcgcctgc agaaagtact tttcaagcga gccctgaaag ctcgagaaca tcgggaacgc
    23401 cgagaacacc gcgagcatca cggtgagcaa tggcaccagc gagatcagcg tggtgaaggc
    23461 caggctgctg gccgtgaggc cgaggcgatc ctcgcgaaag cgctgacgca gcgtgtggac
    23521 cgtgtcaagc cacggccagg tctgcagcgt gaccaaccag agcgccacct gctggcgcac
    23581 ggtgtcggcg accgtggcct tgagcttcgg ggaggactcc atcgctggct atgatgccat
    23641 cgatgagcct gcctccctcc cctccaccgc ccgccacgcc tgccacctca ccgcaagcga
    23701 ggcccgatgc agaagatgcc ggcacgacgc cgatgccgcc ggtggtcgcg tggagccgcg
    23761 cagtggcggt cggcagcctg ctcgggttga tcgtgctggg gctcgcctgg gaactggtgc
    23821 tggcgcctac cggcaaccgc acgctggcgc tcaaggtgct gccgttggcg atcccgctga
    23881 cgggcctgct gaagaaccgc atgtacacct accgctgggt cagcctcgtg atctggctgt
    23941 acttcatcga aggcgtgata cgcgcttcag gcgaccgggg cctgagtgcg tggctggccg
    24001 gtggcgaggt gctgctgtgc gtggtgttgt tcgcggcctg cgcggttcat gtgcgctgga
    24061 ggctgtaccg ggcgcacgcg ctcgctgcgg tcgagcccag cgcaccgacc gcctgaccat
    24121 tcgacacggc agcgacaacg cggtggcgac atggcgctga tcgacgagtt gaactctgcc
    24181 gtcggtgctg ctcaggtgct gaccgaaggc gatctgtcgg cgtgggaaat cgactggcgc
    24241 aggcgctacc gcggacgggc actggcgatc gtgcgccctg ccgacacgct cgaggtggcc
    24301 gccgtggttc gagcctgtgc gcgacatggc gccagcatcg tcacgcaggg cggcaacacc
    24361 agcctggtcg gcgggtcggt gcccgatgcc agcggcacgc aggtcgtgct gagcctgaaa
    24421 cgcatggcac gcatccggca catcgacgaa tccaacctga cgatgacggt ggaagcgggc
    24481 tgcgtgctgg agcaggcgca ggcggccgct gcagaccggg ccctgctcta cccgctgagc
    24541 ctggccgccg agggcagttg caccgtcggc ggcacgctgg ccaccaatgc cggcggcacg
    24601 caggtgctgc gctacggcaa cgcacgcgag ctgtgcctgg ggctcgaggt ggtgacgccg
    24661 gccggcgata tctggaatgg cctgtcgggg ctgcgcaagg acaacacggg ctacgacctg
    24721 cgcgacctgt acatcggcag cgaaggcacg ctgggtgtga tcaccgcagc caccctgaag
    24781 ctctacccac agcccgccgc cgtgaccacc gcgatggcat ccctgtccac cctggaggct
    24841 gccgtgcgcc tgctcgacct ggcgcgcacg cgcctggacg ccggtctgac cggcttcgag
    24901 gtgatgaacg aaacagccct cgacctggtc cgcaggcatt tcccgcaagc gcaacagccg
    24961 ctgccgcgag cggcgtggac ggtgctgctt gaacaatcgg acaccgagag cgagatccat
    25021 gcacgcgtgc gattcgaatc gctgctggag gcggcattgc gcgacggatg catcaccgac
    25081 gcggcggtgg ccggcagcct ggagtaggcg gcatccatgt ggcgcctgcg tgaactcatc
    25141 ccgctggcgc aggccgagga aggactcaac atcaagcacg acatctcgct cccggtgtcg
    25201 cgcatccccg acttcgtgaa acacaccgat gcggtgctgg agacgacctg gccgggcgcg
    25261 cggatcgtcg acttcggtca cctcggcgat ggcaacctgc actacaacgt gcaggcgccg
    25321 ccaggcgtgg cagcagcgga cttcctgcga gccaacgaga cggcggtgaa tgagcgggtc
    25381 tacaacgcgg tcatggcgca cggcggatcg atctcggccg aacacggcat cggcgttctc
    25441 aagcgcgata cgctggccgc ctacaagtca ccggtggcac ttcagttgat gcgggcgatc
    25501 aagctcgccc tggacccgct gggcacgatg aatccgggac gcgtcctctg aaccggaacg
    25561 cggcgcccta ctgcaccgtt cccttgatcg ccgagggaat cggcagcgcc atcacctcga
    25621 tgccctcgtc gtgcagagcc cgtgcctcgt cggaagtggc ctgcccgcgt atcggacgct
    25681 cgtcggcctc gccgtaatgc atgcggcggg cctcttcggc gaattgcgtg ccgacgtcgt
    25741 cggtgtgttt aatgacgtgc tgcaccgctc gcaaccaagc agactgaagc atgaactggt
    25801 cgggctgcgc ttcggcagtc ggtgtaccgg cagcggcaga ttcggtgacc gaacccgggc
    25861 gtggttgggc ctggcgcggc ttcacgggct gggtgtgcgc ctcggcacgt gaggtcgatg
    25921 tcaggacgtg tggcgccgac ggcaggcgct gcacggtcgt gacgccgcac atcgggcact
    25981 cgatgagagc ccgctcggtc tggctgcggt agtcgtcctc ggacgcgaac cagccctcga
    26041 agcgatggtc tgacgggcag cgcaggttca gaaccttcat gcctcgatta tcgcggggcc
    26101 accggatttc atgccgagcg ggttcaccac aaccacacga agcctgactt catctggatc
    26161 gggatgcatc gaccgaaatg gcagcggcag cttcctggct catccacgtc aggccccagc
    26221 ggccctcgcg ccagtgccgc aaccacagca ggccaaccag cgtcttgaca tcggtcaggg
    26281 tgcccaggcg agctgcctcc tccagctcct cgagcgtggc cgtggccacg tcgagaaact
    26341 cgccctcgtc gagctgcgtt gcaccggcag tcagcccgcg ggcgaaccag atttctatgc
    26401 cttctgtcga gtaggcgatg gcgttgtgcg tcaggccggc acgcgcccac tcggtggcgc
    26461 ggtagccggt ttcctcggcc agttcgcgaa cggcacagac gatcggtgcc tcaccggctt
    26521 ccagcttgcc ggccgggaat tcgagcatca cctgcgccac gggatatcgg aactggcgct
    26581 cgatcacgag ccgaccatcg tcgaggaccg gcacgatcat caccgcgccc ggatgaacga
    26641 tgtattcgcg cgacgcagtg gcaccgttgg gcaaacggac gacatcacgc cggacatcga
    26701 ggaaatggcc gaggtagacc tgctccgact cgatgcactc ttcgcgaagg tgggcgtccg
    26761 gctcgctcac cggcgcagcc tcacgtgctc agggccttgc cgatggcgtt gacgcacagt
    26821 gccatcaggc cgccagggag caggccgaac agcagcacgg ctgcaccgtt gagcgacagc
    26881 agcgctcgta cttcacccga agccacgatc ggagcggttt cggcaggctc atcgaagtac
    26941 atgaccttga ccaggcgaag gtagtagaac gcgccgatca gcgagaacac caccgccgcc
    27001 accgccagca cgatgtaacc gtagacattg gtcgaaacga tcgcctgcag cacgctgaac
    27061 ttggcgtaga agcccaccgt tggtggcaca cccgccagcg agaacatgaa gatcgccatg
    27121 acgccggcgt accaggggct gcgcttggac aggcccttca ggtcatcgat gttctcggat
    27181 tcgaagccct gacgggacag cagcatgatg acgccgaagg tacccagtgt cgtcaagacg
    27241 taggtcacca tgtagaacat cgccgagctg tagccgttgg cagccgagag cgtgttggtg
    27301 ccgatcacgg tcggcgtcag tcccagcagc atgaagccca tgttcgcgat ggtcgagtag
    27361 gccagcatgc gcttcaggtt gcgctgcgcg atggcagcca ggttgccgat ggccatcgaa
    27421 ccgacggcca gcacgatcag catctgctgc caatgctcgg cgacaccgga catgccctcg
    27481 accagcagcc ggatcgtgat cgcgaatgca gccagcttgg gcgccccgcc gatcagcagc
    27541 gtgatcgccg tcggtgcacc ctggtagacg tcaggcaccc acatgtggaa gggaacggcg
    27601 cccagcttga atgccagtcc ggccacgatg aagaccacac cgaagatcag caccgccttg
    27661 ttgatctggc ccgtaccgat ggccttgaag acctcgccga tgtcgaggga cccggtggcc
    27721 ccgtacatca tggacatgcc gtagagcagg aatccgctgg ccagcgcgcc aagcacgaag
    27781 tacttcattg cggcttcggt cgcgttcagg ctgtcgcgtc gcatcgccgt cagcgcataa
    27841 agcgacaggc tcatcaactc gagcccgagg tagaccacca ggaagttgtt cgccgacacc
    27901 atcacgcaga tgccgagcag cgagaacatg ctgagcgtga aaagctcgcc cttcagcatt
    27961 tcgcgcccgt cggcataggg acgagcgtag gccagcgaga tcatcaccgc gacagtggcg
    28021 aagaatgcca gcacattgcc catcgggtcg gcgaccacca tctgctgcat cgcgtagacc
    28081 gagagtccgg cgtcgtactg gtagagaaac atcaatgcga cgatggccag cgaaccctgg
    28141 gtgaggcggt acgtgaagac gcgcttggga tcggtgaccc acaggtcgac gagtgccacg
    28201 acgcaggtcg ccaggagcag gaagatttcg gggtagacgg caagccagtt catgtcgttc
    28261 atcagttcag cttcgacacg gccacgtgct tcagcagttc agtcaccgac acatgcatca
    28321 catcggtgaa cggcttcggg tagagaccca tccacagcac ggcaaccgcc agcaccgtca
    28381 tgatcagcat ttcacgggaa ttgaggtcga tcagttcgcg cacgtggtga ttgccaatcg
    28441 gtccgaaata cacccgcttg accatccaca gcgtgtaggc agcaccgaac accagtgcag
    28501 tggctgccag cgcaccgagc cagaagttca cctgcacggc cgccaggatg accatccact
    28561 cgccgacgaa gccggcggtc gcaggcaagc cgcaattggc catcgcgaag aacacggcga
    28621 aggccgtgaa cgtcggcatc gtgttgacga cgccaccata gctggcaatt tcgcgggaat
    28681 gaacccggtc gtacaggacg ccgatgcaca ggaacatggc acccgagacg aatccgtgcg
    28741 aaatcatctg caccagcgca cccgagacgc cgatctcgtt gaacaggaag aagcccagcg
    28801 tgacgaagcc catgtgcgcg atggacgaat aggcgacgag cttcttcatg tcctgctgaa
    28861 ccagcgccac gagaccaatg tagatcaccg cgatcaagga gatcgtgatg atgaaccagg
    28921 cccactcgtg agaagcatcg ggtgtgatcg gcagcgagaa gcgcaggaat ccgtagccgc
    28981 cgagcttcag catgatggcg gccagaacca ccgagcctcc ggtcggggcc tcgacgtgcg
    29041 cgtcaggcaa ccaagtgtgc accggccaca tcggcacctt gaccgcaaag
//
LOCUS       DAOORV010000029        28998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1869_length_28998_cov_6.581591, whole genome shotgun sequence.
ACCESSION   DAOORV010000029 DAOORV010000000
VERSION     DAOORV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28998
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_28998_cov_6.581591"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKV56_05915"
     CDS             complement(<1..391)
                     /locus_tag="PKV56_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HOX67383.1"
                     /translation="MKLRPAHPKPVISGIVDQPPSALKPWANNPRRHSPKQLFKLKQS
                     IDYFGVTNPILVDENGVILAGEARWQVAQSLNLATVPTRVITGLSKAAKRAYVLTDNK
                     LALLSSWDMGLLRDEIQLLIADDFEIEL"
     gene            complement(1334..1909)
                     /locus_tag="PKV56_05920"
     CDS             complement(1334..1909)
                     /locus_tag="PKV56_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67384.1"
                     /translation="MDLLPEATASRVQERRVVAEDLAARLEGAQVDRVRLLKALLERV
                     VVRDDAIDIEIKLGALDNDAAERTHALKVPAQLKRCGLGMRLVIPGRIDPGSGQPDAK
                     LMALLAKARRWFEMLRTGACGSIGELARDVNMAGSDVTQIVYLAFMAPDLIERIAAGA
                     QPPGLGVRKLLAASPLPLDWAEQRRVLGFDA"
     gene            complement(2050..3774)
                     /locus_tag="PKV56_05925"
     CDS             complement(2050..3774)
                     /locus_tag="PKV56_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOX67385.1"
                     /translation="MPMKRPGPLPADTIFGVEDQPPWTTVAVAAVQHIGLLATNFVVA
                     LVVLEAAGVTEDTRNGAISMVMIMCGLGTLLQACRRVPVSSGYLVPIVASAVYVGPAL
                     AAVQLGGLPLLAGMLMFAGAVEMALSRALHRLRPFFPPEIAGLVIFLVGTTIAVLGVR
                     DMFGIGFGTPPSLMHGWIAALTLGLMVALQVWGGAWGKLAGPLIGVVLGYAAAAAGGL
                     VSVDAFAHVARLPLIAAPDLSHLGWAFDPRLMIPFALAALASAVKTMGTIALAQRVND
                     ATWVRLEMKSARRGVLGDGLTTFLGGGLGSVVGANPGSSNAGLVLATGVASRSVAWPT
                     GLLLIAMAFIPTASVLLIQVPKPVSGAVLAFSACFIMSSGIQMIASRMLDARKTLVIG
                     LAFGAGLSAEVFPDLARHVPDWLGPVVGSPLVCGMLLGLCLNAAFRIGVRQHATLRLP
                     TKGDRVHALKHFIELQGERWGARRDVMHRAEYALDQLIETVIEHGEPRGDITVSLSFD
                     EFSVAAAVDYLGIGLQFPEQRPSADEIVDTLDGVRRLAGFLLRRSADRISTVALAEGS
                     RVEMIFDH"
     gene            complement(4331..4840)
                     /locus_tag="PKV56_05930"
     CDS             complement(4331..4840)
                     /locus_tag="PKV56_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67386.1"
                     /translation="MHLPRRFDDALMRAPDARGPADAALIAMLRAWCEGGSVPTLAEP
                     LRAASIAPHAGLDGAACVLDGSQDLARLGRWLGLRWRLQILWREQVAGYSAQTSDPWD
                     CGWWREGPLGPAEVFRPRRATLMMLREPAPAAASALLATLRAHSPSYTKPLRVLVVSA
                     VPMGGMARL"
     gene            5388..6806
                     /gene="ccoG"
                     /locus_tag="PKV56_05935"
     CDS             5388..6806
                     /gene="ccoG"
                     /locus_tag="PKV56_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOX67387.1"
                     /translation="MSQTQRVIPILPDTAAAAASPAMRIQPRAVRGNFARWRWALVWI
                     TQGFFFGMPWLDWNGRQAVLFDLEGRRFYFFDAVLLPQDLILLTGVLVFSALLLFAVT
                     ALLGRIWCGFACPQTVYTEILLWIEHHIEGDRLTRLRLDAAPWNREKLLRRGGKHLAW
                     AGAALLTGFTLVGWFTPVRDLAAAVPSLAFGPWELFWMLFYGAATYLLAGLLREKVCQ
                     HACPYGRFQGSMLDPATRVVAYDDLRGEPRGARARGVAAHACGQGDCVDCTMCVQVCP
                     VGIDIRQGLQLACISCGLCIDACNEVMDKMRAPRGLIRFQALARTVKTVPWWRPRVIV
                     YAASLLLVGSSVVWAWAARPELRLNVVRDRGVLSRQVDDGAIENVYRLQLMNASLRAR
                     HLAVSAFIDGGEAVPLQVSQARSLVIEPAGTTTAALTLHMDELTAQRQGPGRVVPIRI
                     EVRDADGGGAVHAASASTFMLH"
     gene            complement(6822..8252)
                     /locus_tag="PKV56_05940"
     CDS             complement(6822..8252)
                     /locus_tag="PKV56_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX67388.1"
                     /translation="MPGLSTSRYEQLAHDVTQLIREGLLKPGERLPSVRDTCQSRGVS
                     PSTVFQAYGLLELQGLIEARPRSGYFVRNQRRAPRALPLPAVPKPEATDVVVSELAFD
                     LLESTRDPAVVPLGSAFPAAHLFPFDALARSGARAMRRVKPQSITSALTAGDESLRQA
                     LRRRYALQGVALAEQELVVTNGAMEALNLCLQAVTSPGDLVVVESPTFYAALQALERL
                     NLRAVEVATDPTDGVDLAALAELLARQPVKACWFMPNFQNPLGALMPVARKQALVTLL
                     ARHGVPLIEDDVYGELHAELRRPPPAKAFDVQGLVLHCSSFSKCLAPGYRVGWAAAGR
                     YARTVQRLKMMSSLATALPPQLAIADYLAQGGFDRHLRQLRSTLALEQQRVRRLIERH
                     FPSGTRVTRPAGGYFLWLELPPAVHALTLHQQALSLGISTAPGVLFSADRRFAHHLRL
                     NAGHPGDDRIDPALRRLGELACQALR"
     gene            8550..9173
                     /locus_tag="PKV56_05945"
     CDS             8550..9173
                     /locus_tag="PKV56_05945"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOX67389.1"
                     /translation="MNGPFTPRRAVCVCCLRPQLTCLCALARPTAHRTEVLVLQHPQE
                     QRQAKNSVAMLRLSLAHCEVVVGERFAPEVLAALLQRPGRDTRLLYPDVPAAPAPSAP
                     GTTAGAPLRLVVIDATWRKSLRMLLEHPALAALPRLSLVAPEPTCYRAIRAARRADQV
                     STLEATVQALAMLEGPSFDAAPLLDAFGCFVAGEAARQPPRASSGQA"
     gene            complement(9387..9608)
                     /locus_tag="PKV56_05950"
     CDS             complement(9387..9608)
                     /locus_tag="PKV56_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67390.1"
                     /translation="MKGFLSSRWSKVGVAFVVFGWGPLLLIIFLAAIGLWPDPNPNPI
                     GPGLLFAVTVWPALICLGIGACKTLRKRS"
     gene            complement(9799..10167)
                     /locus_tag="PKV56_05955"
     CDS             complement(9799..10167)
                     /locus_tag="PKV56_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67391.1"
                     /translation="MAALSVGCRAVVCAVEEHVNFASTELWHDGHRVWHVQHEGDRDV
                     ENISHAGAVPARFHELLATVEPGDSEDFEGHFHMDIPLILAKDITGFLHDECNPEVDD
                     TPFEQLTDLAAKRQWWRLWK"
     gene            complement(10446..10904)
                     /locus_tag="PKV56_05960"
     CDS             complement(10446..10904)
                     /locus_tag="PKV56_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67392.1"
                     /translation="MNLHLPKAVFIAVAWAAMLPTAFAQDAHRRAAQELHKFLVAADA
                     TTAIQLVASAMGAQDPMLRKHKAIVESWLSDQIASRAYAEAKIASYMEVYSEEELTLV
                     LAMVKTSAFRVFQAKHQQTLRSSAPKLVQLARESEPELERRLQQAERSTK"
     gene            11290..12687
                     /locus_tag="PKV56_05965"
     CDS             11290..12687
                     /locus_tag="PKV56_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOX67393.1"
                     /translation="MDRLLFPESAVAPQARVLRMSELLGALSRALDITEGQPPGHCVR
                     SCWIGMRIGQVLGLPGLALWELYYTLLLKDLGCSSNAARICELYLTDDRRFKQDFKTV
                     GDNLPQMLHFVFTHTGLKAGLAQRLGAVLNIMRNGPEIATELIQTRCTRGADIARQLR
                     FPTAVCDGIAGLDEHWNGQGKPQGLRGKQIPLYSRIALLAQVVDVFHSTGGPQAALME
                     VARRAGTWFDPELVRAIASVGQDSAFWATLASPGLDQAVQALEPGAHVVPLDESYLDD
                     IAEAFGQVVDAKSPFTSGHSVRVAAYTERIATKLGLSTARTRWLRRGALLHDVGKLGV
                     SNSVLDKPGKLDAEEWGAVQLHAAYTQDILGRIGAFEELAGISAAHHERLDGKGYPLG
                     LAGDQISIETRIITTADIFDAISAERPYRSAVPIPRTLEMMAENLGTAIDPLCLDALT
                     AVLLQEGQLHGGVNH"
     gene            complement(13149..13325)
                     /locus_tag="PKV56_05970"
     CDS             complement(13149..13325)
                     /locus_tag="PKV56_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67394.1"
                     /translation="MTQVADLALLVPDSMQKIMSGALPLGVNIKCMLAASPLPLDWAK
                     QRQALGFDGWRRGG"
     gene            complement(13344..13856)
                     /locus_tag="PKV56_05975"
     CDS             complement(13344..13856)
                     /locus_tag="PKV56_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67395.1"
                     /translation="MKPTFTPRQSCPVLLTLAAGAIFALGSAPALAQTRAALVQSVDE
                     PGRNHYQEVLSDLGCRGSTVCSFSFGTVPAGKRLVVTQIAGYVDTANGTLPNGFLQSS
                     FGGSAYATLPFTGTRGPATPLGTRIFINHGVLAYFGAGENPRATYQVFSDSMSGGALM
                     MLTGHYVNAP"
     gene            complement(13856..14470)
                     /locus_tag="PKV56_05980"
     CDS             complement(13856..14470)
                     /locus_tag="PKV56_05980"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HOX67396.1"
                     /translation="MALAVFAGPVLAGLVTLNGSLADPANAALVASNMSAAQFGDDID
                     TANNVALYELHVAVGGNVNFTSTGFIPGGIDPYFTLFSGTDRAIATFRESNYLQAVSV
                     GGDFTQDVVLTAGDYTVAISVFENMSFAENSGGFLADGFIGLGGPLYLGDGSYRLAIT
                     LPDVVGIVPEPGSAVLVLTAALAAAWARRRRHSVPPPLTTHGER"
     gene            15232..15672
                     /locus_tag="PKV56_05985"
     CDS             15232..15672
                     /locus_tag="PKV56_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67397.1"
                     /translation="MKCCEGWLAGPVRREGVVDHVLMRGSPCPHATSGRCAIYDSRPE
                     DPCKSFVCGWLMPASPLPESYRPDKLGVIFVQLVWRQRRAWALVPAGGDIGEELRASM
                     RSYTQATGEPHLLKYPERLLCYGSAEFQQDMVAKAERGENPWDP"
     gene            15811..16374
                     /locus_tag="PKV56_05990"
     CDS             15811..16374
                     /locus_tag="PKV56_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOX67398.1"
                     /translation="MTNARRNFHKTAFTTASALLLGTLGVNASAASAEDLDKDAAQAL
                     LTLYKSRPVAEVVSKKAKAILVFPKVLKAGLVFGGSYGEGVLMKGSHVTDYYNSVSAS
                     WGLQAGAESYAYFVFLMSDKAVRYLEKSKGWELGVGPSLVIVNEGVAKNLSTSTLKDD
                     AYAFISDQQGLMASLSIEGTKISRIKR"
     gene            complement(16468..17166)
                     /locus_tag="PKV56_05995"
     CDS             complement(16468..17166)
                     /locus_tag="PKV56_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOX67399.1"
                     /translation="MNTQYSSPVSGYGGSSATVEQRNRVLRNTYWLLALSMVPTVLGA
                     WIGISTGLSRAMSPGIGLIVFMVGAFGFMFAIEKTKNSAAGVPMLLAFTFFMGVMLSR
                     LVGSVLGLANGASLIMTAFAGTGAIFLGMATLSSVIKRDLTSMGKFLFIGAIMLLVAG
                     IANIFLQSSALMITLAVLAIGIFSAFILYDLKRVQDGHETNYITATLGVYLSLYNVFQ
                     SLLALLGIFGSREE"
     gene            complement(17163..17972)
                     /locus_tag="PKV56_06000"
     CDS             complement(17163..17972)
                     /locus_tag="PKV56_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOX67400.1"
                     /translation="MEFLSDPNLWIAFAMLTALEIVLGVDNIIFISILVGRLPVHLRD
                     QARRLGLGFAMVSRLLLLFSLSWVMGLTTDLFNLFGQGISGRDLVLLFGGLFLLWKAS
                     HEIFVEVEASEQQGPPATDSEQLHAAGRRLFWGVIGQIAIIDIVFSLDSVITAVGMVD
                     QISVMVAAVVTSVAVMMVAAKPIGEFVDRHPSIKVLALAFLVMVGTALTAEAFDAAIP
                     KGYIYAAMAFSLAVEALNIRARGKRQARTLQADPASQTTSLASTSSLKETP"
     gene            complement(18065..18430)
                     /locus_tag="PKV56_06005"
     CDS             complement(18065..18430)
                     /locus_tag="PKV56_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67401.1"
                     /translation="MSSKFVALLLALVVCWSGFLGLEQTRTLALANAEQQIDSPWTAD
                     PLKRMAGASTNTQHPDELPAPSHAEAAADPHAILTGCVQVTTPLLAMARPNSYAAPTR
                     LSPDLDGPQRPPRTRALLA"
     gene            complement(18558..21188)
                     /locus_tag="PKV56_06010"
     CDS             complement(18558..21188)
                     /locus_tag="PKV56_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX67402.1"
                     /translation="MIATWLHKARTNTRPPSPRIVIALAAIVLLLIVESLIVSDVNRT
                     RDHALEVLESTARQETEAKSRELSQVLADLYVTTRTIAMLPAVRRPAAQNRASDKDDV
                     IDGRRFTTSDAQTVTQLYLHLADLMSVSEIYIVYDGFKPHRGEVPFLMFDSVIVDRFR
                     HLSQTARESTHGHLLGEAPEQVEEEEYAQIVHQLNQFRRDHPTLPAQAPHGMPSLISE
                     PIITCDNSQFLSTSGSQQRDRTGMMLSVPIYDEETGAFKGLVTTVVRLNVLEARLLGW
                     PNIPVTPEERSLARGAGLDKTPAADYALTHEASSVAVTDRRNAGLQDILAGNTKAGLT
                     IRDPLEGPMSSSWVLSRHVPQASFDAIHATARHDLIVGSLIAIGTLTTLAVIALIFIS
                     QRRNAALLKEQADYDALTGLPNRRQLDRTLDAGLRSATMARSPLMVVMVDLDNFKTVN
                     DVHGHHVGDLLLIEVARRFQKQLRSTDVILGTGDPGRGGMAPSFGRLGGDEFLIVLPG
                     MSGEAAAHSVAERLLSSLAVPMLLDGRTIQARASMGVAVYPDHGETVAQLMRCADQAM
                     YQAKRVDDSAVILFQREVDPQAMRRIRLTRDLREALLQEQFELHYQPVVNLTQERVDS
                     VEALLRWRHPEFGLVSPGEFVPLLESSGVIVPVGLWALRKACKQLMAWRSSGLTIESI
                     AVNVSFVQLSHSNFSNDAMAVIAGTGIDPSRVTVEVTESVLMENPERSITQLCELRDA
                     GVRISIDDFGTGYSSLSYLQRLPVHSMKIDRSLLVDAVSPAGRTILSTMVRLSIELGL
                     DCVVEGVETLEQYQMLREIGCRRLQGYLFSRPLPADEAEAAASRINQQQFNVAGADDY
                     AEADVAHHRHGTAPAAVWVN"
     gene            21497..23302
                     /locus_tag="PKV56_06015"
     CDS             21497..23302
                     /locus_tag="PKV56_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOX67403.1"
                     /translation="MRPLLLACLCWMLSFASPAFAMSPPDGLNDLRPIDESQWTSASA
                     AHLLQRAGFGGRPDEIAALTRLGPREAVRRLVRWQSVPDNFVPFDESDLHDPGLEPFP
                     ASRPATTDAAKRDGQAIGVAAKPAGNNRRLQPVTNKFFYWLRASRLETHRLSYWWAQR
                     MLVTPRPLQEKLALFWHGHFATSEEKVRDYRKMLRQNMLFREKGNGSFRELMIGVAQD
                     PAMLSFLDAGVNVKGAPNENFAREIMEMFTLGVGQYSEHDIREAARAFTGWNAKDLTF
                     TIDAARHDDGIKRLLGREGPLDGIDVIDTLLAQPAAGEFIAGKLYMYFVRQEVSPALR
                     TELGSRLRASGYDIADLLETIFMSRDFYSQATVASRVRSPVELVVATYRQAGLTQVPG
                     VPDFNEVTDSLGQKLFYPPTVAGWAQGRSWITPGLLIARSNFAYDLVFPDINFIPPDR
                     YPSADFKIRDFSDKLALGFDVTTASKLEGTDMSSMSNQMADRDEEFNTRLASFRGWQM
                     ALERVKPIPRAPARLELTRLVLGDGCRTAGDAVDALLRRFVSAPVAPATRARLTEFLQ
                     QELGTPDLAAARSYLDEPLRQTLHLILSLPEYQLG"
     gene            23310..24536
                     /locus_tag="PKV56_06020"
     CDS             23310..24536
                     /locus_tag="PKV56_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOX67404.1"
                     /translation="MNKSNPVDGLLSRRSALKAGLIGTGWLGGVPGVLQQACAATSAR
                     DDKRILVVLELSGGNDGLNTLVPYGDDAYYRHRPKLGIRRQQLRVIDDHHGFSPGMAG
                     FERLYKDGRMAVVHGCGYTQPSFSHFTSAAYWHTAMPNSGEEYGWIGRLADVIDPKLT
                     PNFVVNIDETQTLAVRARNHLPVSFDDPNRFVRKGLYEERAVLDAAPVVQPQDSASLR
                     FMRDVAVSARESSQIVREACARYKTPIDYGISPLDLPKIAALIAADLPTRLYYCAYRR
                     NAFDTHVQQAELHQRLLTYASDAVSAFMRDLERIGRSKDVTLLVFSEFGRRVPENTSL
                     GTDHGTANHMYVIGQPVRGGHYGTLPSLTKLDEGDNLIHTTDFRRVYATLIEGWLGAD
                     AQQVLRGRYEGFPMFG"
     gene            complement(24541..25494)
                     /locus_tag="PKV56_06025"
     CDS             complement(24541..25494)
                     /locus_tag="PKV56_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX67405.1"
                     /translation="MSTELRSRAGLTPHPKPTPASPDASASTLAPALSGECIAFDGLN
                     CYVAGHGPPLLLVHSVNAAASAAEMRPLFDRYRYTRTVFAIDLPGFGLSDRSDRDYTP
                     RLMTDALHSTVAEIRRRCGPLPIAGVAVSLGTEFLARAAVERPDQWDRLALVSPTGLN
                     GHQARRGPEGSTRGIAGMHALLSVPLWSESLFRLLTRPAVVRYFLERTWGGRVIDEKL
                     WAYDVQTARQPGARFAPLHFLSGNLFSADIHRVYEGISQPVWMSHGVQGDFTDYRNQR
                     IVEARSNWRIDTFKTGALPYFEVPDEFFAKLDLFLSGPAPH"
     gene            25567..26349
                     /locus_tag="PKV56_06030"
     CDS             25567..26349
                     /locus_tag="PKV56_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67406.1"
                     /translation="MAGSIIPQKCRMRREYCRTGLLLLWPAGAYSRTEGFFACPPLFS
                     SGGLMSSLPQTESEASAASHSTATVARRLGVSIPTVQRWVDQGHLKAWKTVGGHRRID
                     AASVDLFIQRREPQDLAGSLIQDASIAPFVLIVDDNPVDRELLVGLVEEALPAAAITA
                     VGSGFECLIAVGQRTPDVLITDIMMPHMNGFEMLRHVATRCAVRPKVVIAVSGRSPEQ
                     FAELGELPDDVQFLAKPLNADVLIDLLRACADASGLRAIARA"
     gene            complement(26418..26996)
                     /locus_tag="PKV56_06035"
     CDS             complement(26418..26996)
                     /locus_tag="PKV56_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67407.1"
                     /translation="MLQIHLFRHLSACALLTWAASLPLAASAWDLSGTRTMTLHARNG
                     DAVKIGTVQFTPQADGRTAFVVTTATENFKDFFLSMKEFKCVEGSGEILCHVPYPYPQ
                     PGTVTASDFAWLEHSLMFMYKTPAEFGARLWNGIYFRLRLTEQGLEGQPQAIDLNQIS
                     APPDKTDVPPYRPALRDDIPAGQRWFNRLTIE"
     gene            complement(27156..>28998)
                     /locus_tag="PKV56_06040"
     CDS             complement(27156..>28998)
                     /locus_tag="PKV56_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX67408.1"
                     /translation="ELLSAGAPVMAHARQFLDQTGTVMVLTTNEGIVLNLEGDPSTFG
                     AAERIHLSPGSSWSEATVGTNAIGTALAIGQPVQIHSAEHYCAGIKRWTCSATVVRDP
                     FDNSILGVVDVSGLNSTYNRHSLALVVTTASRIESRLARRELELRYHLLERCMGRLTS
                     ARTDGVIVLDRHGIPIKASDSAEIALRALSNARAGGSAVDLSNLKIASPIRNSPPVDL
                     PDWISRDWLEPVFEEDQWLGSLLTIPHRGSPQSPTRRGNPAGSKHSFLKNAAFERVIG
                     RAPNLLETIGSAQRLARSSVPILLLGETGVGKDIFARAIHETSASSAGPFVALNCGGL
                     SRELLTSELFGYSEGSFTGARRGGMMGKLEAADGGTLFLDEVGEMPLDLQPHLLRVLE
                     EGEIYRVGEVKPRKVKFRLIAATNRDLRAEVSEGRFRMDLFYRIAVASIDIPPLRSRP
                     GDIELIAEFLLDKLAAQHTVPRPSIDPRALECLFQYAWPGNVRELRNVLERALLMSSE
                     NVLRKDALPDEIRLPRRILAQRDSVASAPGSSLNRLKQAERETLQSMISRQSGNMTAV
                     ARELGIAKSTLYEKMKKLEIPLTGGRCADDGTGAEIHELRGRAARPI"
BASE COUNT         5070 a         9247 c         9575 g         5106 t
ORIGIN      
        1 tcagctcgat ctcgaagtcg tcggcgatca gcagctggat ctcgtcgcgc agcaggccca
       61 tgtcccagct ggacagcagg gccagcttgt tgtcggtcag gacataggcc cgtttggcgg
      121 ccttggagag acccgtgatg acgcgggtgg gcaccgttgc gagattgagg ctctgggcaa
      181 cctgccagcg tgcttcgccc gccagaatga cgccgttctc atcgaccagg atgggattgg
      241 tgacgccgaa atagtcgatg ctctgtttga gcttgaagag ctgcttgggg ctgtgacggc
      301 gcgggttgtt ggcccagggc ttgagggccg agggcggctg gtcaacgatg ccgctgatga
      361 cgggcttggg atgggcaggt ctgagcttca aggcattctc caaaagttgc atggagaccc
      421 gcgctgcata aggtgccgct tgcccgtctc aacgccgctt caaaagcgtg catgagtcgg
      481 ccagccgaca ccctatgcac tccattgacg cgggtcttga acgatcagca taggttgccg
      541 aaatccacct agctagcagt tgcaactacg cgttgaaggt gcccgcaaag atttgggctt
      601 gcggcgttag cacatgggga accacctagc cagcgagaaa gcaccctggc tcggtgacaa
      661 gtcgaggctg ccgagtgggg aggtgcaggt gaggcggagc aggcggtcga tagcacagtg
      721 gcatccacct ggcgagggct tgcgatgcag aggcagcggc tggaggtggc gctttcctag
      781 cgtgcgccga accacctagc aagggactgg cgcgaacttc gagccagggc ggtttggtgg
      841 tcgcgcagtg cgaaagagtg cggcgattcg ctgaaagcca gagctgaatt ccctgatgcg
      901 ggtagacgaa ttccctgttc gttaactggg ccgtgagacg gggtggtggg ttttgcctgg
      961 cacgaccgag tcaaagtggg ccggctgggt ctttccgcgg taaggaagtc cctgttgttt
     1021 tccctgttgc agggaattca ggctactttt gagacgggtt cgctgttgac tgcgcccacc
     1081 gccaacattt caaagccacc gcagcgcggt ggctttttct ttggagaagc ccgcgcggcg
     1141 cgggtctccg ggcggtgcag ctttctctgt gggctgcgag cggcaccatc actgctactt
     1201 tcagcccggc tttctcagtc ccgaagattg cgttccgcac tgttgacggg tggcatcact
     1261 gccgacacca gtagcctgct gccagaagcg ggaagagggc ctttcggccc ccggtgtttc
     1321 cagtctgtcg aggctacgcg tcgaacccca gcacccgccg ctgctcggcc cagtccagtg
     1381 gcagcggaga tgcggccagc agcttgcgca cccccaaccc gggcggctgc gcccccgctg
     1441 cgatccgctc gatgaggtcc ggcgccatga aggccaggta cacgatctgc gtcacatccg
     1501 agcccgccat gttcacgtcc cgggccaact caccgatgct cccgcaggcg cccgtgcgca
     1561 gcatctcgaa ccagcgcctg gccttcgcca gcaaggccat cagcttcgca tccggctgac
     1621 ccgatcccgg atcgattcgc cccgggatca ccaaccgcat ccccagccca cagcgcttca
     1681 actgcgccgg caccttcaac gcatgggtgc gctccgccgc atcgttgtcg agtgccccca
     1741 gcttgatctc gatgtcgatt gcatcatccc tgacgacgac tcgttcgagc agtgccttca
     1801 gcagcctcac ccgatccacc tgagcacctt ccagtcgtgc agccagatct tccgcaacga
     1861 ctctgcgctc ctgcacccga cttgccgtgg cttccggcag aagatccatg acgcgatgct
     1921 cagcgtgcga ccgagatcgc ttttctgccc gacgtgaagg tgccttgcga cctgtgccgc
     1981 ggcgcgcttc aatccggtaa ttctgggccg tcgcctggcg cacggtcgct cgccgtgcgg
     2041 caccggtctt cagtgatcga agatcatctc gacacgggaa ccttcggcga gcgccaccgt
     2101 gctgatgcgg tccgcactgc gacgcagcag gaagccggcg aggcgacgaa cgccgtccag
     2161 cgtatcaacg atctcgtcgg cgctgggccg ctgttccggg aattgcagcc cgatccccag
     2221 gtaatcaacc gcagccgcga cgctgaactc gtcgaacgac aggctcaccg tgatgtctcc
     2281 gcgcggctcg ccatgctcga tcacggtctc gatcagttga tccagcgcgt actcggcccg
     2341 atgcatcaca tcgcgccggg caccccagcg ctctccctgc agctcgatga agtgcttcag
     2401 cgcgtggacc cggtcgccct tggtcggcaa ccgcagcgtg gcgtgctgcc gcacgccgat
     2461 ccggaacgcc gcattcaggc acagccccag cagcatgccg cagacgagtg ggctgccgac
     2521 caccggtccc agccagtccg gaacgtggcg ggccaggtcg ggaaacactt ccgcgctcag
     2581 accagccccg aacgccaggc cgatcacgag cgtcttgcgt gcgtcgagca tgcgtgaggc
     2641 gatcatctgg atgccactgc tcatgatgaa gcatgcggag aacgccagca cagcgccgct
     2701 gaccggcttg ggcacctgaa tcagtaaaac cgaggcggtc ggaatgaagg ccatcgcgat
     2761 cagcagcagg cccgtcggcc acgctacgct gcggctggcc acgccggtgg ccagcaccag
     2821 tccggcattg ctggaacccg ggttggcgcc gacgaccgag cccagcccac cgcccaggaa
     2881 ggtggtcagt ccgtctccca acacgccgcg gcgggccgac ttcatctcga gccgcaccca
     2941 cgtggcgtcg ttgactcgct gagccagcgc gatcgttccc atcgtcttga cggccgaggc
     3001 cagcgcggcg agcgcgaacg ggatcatcaa gcgcggatcg aaggcccagc ccaggtggct
     3061 caggtcgggt gctgcgatga gcggtaagcg ggccacatgg gcgaaggcat cgacagagac
     3121 gaggccgccg gctgcggctg cggcgtagcc gagcacgact ccgatcagcg gtcccgccag
     3181 cttgccccat gccccgcccc aaacctgcag cgcgaccatc agcccgagcg tcagcgcggc
     3241 gatccagcca tgcatcaggc tgggcggagt tccgaatccg atgccaaaca tgtcgcgcac
     3301 gcctagtaca gcgatggtgg tgccgacaag gaagatcacg aggcccgcga tttccggcgg
     3361 gaagaacggt cgcaggcgat gcagtgcgcg agatagggcc atctccaccg cgcccgcaaa
     3421 catcagcatg cctgccagca gcggtaggcc acccagttgc accgcagcca gggcgggtcc
     3481 gacatagacc gccgaggcca cgatcggcac cagatatccg ctgctgaccg gcaccctgcg
     3541 gcaggcctgc agcagggtgc ccagcccgca catgatcatc accatgctga tcgcgccgtt
     3601 gcgggtgtcc tcggtgacgc ccgcagcctc cagtacgacc agcgcgacaa cgaagttggt
     3661 cgcgagcagc cctatgtgtt gtacggcggc gactgcgacg gtggtccatg gcggttgatc
     3721 ttcgaccccg aagatggtgt cggccggcag cggcccgggt cttttcatag gcatggacgg
     3781 agtatggtgc gacgccggta agcggcgcag atgcctcaag cgtcagtgtc gaaggtgctc
     3841 gcaaagcctt gaactggtgg ctgcagcaca tggggaactt ccgagccagt gaggtgggtc
     3901 gctgtcgaaa ttcggacagg ggctgcccga catcacagcg gttgcgtccg agccagcgag
     3961 aaaggcgggg tgcggcgcgc aggtcaagaa agtacggcgg cgactcgctg aaagcgggtc
     4021 ttgaattccc tgacaccggt gaatgaatcc ccgttcgtga agcgagctgt gagaggggac
     4081 ctgcgggatt gcctgggaag agggcgccga agtggcacgg ctgggttcgc ccgcggcacg
     4141 gaagtccctg tatttttctt tggagaagcc cgcgcggcgc gggtctccgg gcggtgcagc
     4201 tttctctgtc ggctgtgcag gccatcatca gcgcgacttt cagatcggct tactcagtcc
     4261 cgaagattgc gttctgcact gttgaccggt gggccgtacg gcagccactt gctgcgcgca
     4321 ttcccggcag ctaaagccgt gccatgcccc ccatgggcac ggcggacacc accagcactc
     4381 gcagcggctt ggtatacgac gggctgtggg cgcgcagcgt ggccagcagt gccgacgcgg
     4441 cggcaggggc cggctcgcgc agcatcatca gtgtggcccg gcgcggtctg aagacctcgg
     4501 ccgggcccaa cggcccttcg cgccaccagc cgcaatccca ggggtcgctt gtctgagcgc
     4561 tgtacccagc cacttgctca cgccacagaa tctgcaagcg ccaccgcagg ccgagccaac
     4621 ggcccaggcg cgccagatcc tggctaccgt ccagcacgca ggctgcgccg tccagcccgg
     4681 catgcggtgc gatgctggct gcacgcagtg gctctgccag cgtcggcacg gaaccgccct
     4741 cgcaccatgc ccgcagcatc gcgatcagcg cagcgtcagc cggcccgcgg gcgtcgggcg
     4801 cgcgcatcag tgcatcgtcg aagcggcggg gcaagtgcat ggcaaggtga ctcttcttat
     4861 gcccattgca gcacagcgcg acagcctgtt gatctgaacg ctgcgcgtgc tggtggtgtc
     4921 cgcggtgccc gtggtgggca tggcacggct ttagctgcct gactgtggcg ggccattgcc
     4981 cgagtgcggg cctgcatgct tgaactcgtc cgtgatccag attcaaccgc gttgtgccag
     5041 ctacgagtcg agatgtcgaa ctcgccgcat tgaagtccgg ccactcgatc tctgtcgcac
     5101 tgaaaggctg ctgtcggcgt tcgtggcgag ctgacagtgc cggccaagag tggccgcagg
     5161 atgccgctgt ctggccgttc gcttcacgct gcttcccatg attttcgagc cggttgccgc
     5221 ggccggggcg atcagctgaa gccattctcg agcagaccag ggattcggcc cgtgcaactg
     5281 atctggtcga aattccactt tcctgagcct atgcaatcag atggccggcg agcaagctcc
     5341 tgtctgcgtc ggggcacctt cgatctcccg acgggaagca gccaaagatg tcccagactc
     5401 aacgcgtcat tcccatcctg cccgacacag ccgcggcagc tgcgagcccc gccatgcgga
     5461 ttcaaccccg cgccgtccgt ggcaactttg cccgctggcg ttgggcactg gtctggatca
     5521 cgcagggctt cttctttggc atgccctggt tggactggaa cgggcgtcag gcggtgctgt
     5581 tcgacctgga gggcaggcgc ttctacttct tcgatgcggt tctgttaccg caagacctga
     5641 tcctgctgac cggtgtgctg gtcttcagtg cgctgctgct gttcgccgtg acagccttgc
     5701 tcgggcgcat ctggtgtggt tttgcgtgtc cccagaccgt ctacaccgag atcctgctct
     5761 ggatcgagca tcacatcgaa ggtgaccgcc tgacccggct gaggctggat gcagcgccct
     5821 ggaacaggga gaagctgttg cgacggggtg gcaaacacct tgcctgggct ggggcggcac
     5881 tgctgaccgg cttcacactt gtcggctggt tcacgccggt gcgcgatctt gctgccgccg
     5941 tgccaagcct ggccttcggt ccctgggagc tcttctggat gctcttctac ggcgccgcca
     6001 cctatttgct ggcgggcctg ctgcgcgaga aggtctgtca gcacgcctgc ccctatggcc
     6061 gcttccaggg ctcgatgctc gaccccgcca cccgcgtggt ggcctacgac gatctgcgcg
     6121 gtgagccgcg tggtgcgcgc gcgcgcggcg ttgccgccca tgcatgcggg caaggtgact
     6181 gcgtggactg cacgatgtgc gtgcaggtct gcccggtcgg catcgacatc cgccaaggcc
     6241 tgcaactggc ctgcatcagc tgcgggctgt gcatcgatgc ctgcaacgag gtgatggaca
     6301 agatgcgggc gccgcggggt ctgatccgct tccaggcctt ggcgaggacc gtcaagacgg
     6361 tgccttggtg gcggccacgc gtcatcgtct atgccgcaag cctgctgctg gtgggaagct
     6421 ccgttgtctg ggcctgggcg gcgcggccgg agttgcgtct gaacgtcgtg cgcgaccgtg
     6481 gcgtgctgtc ccgacaggtg gatgacggtg ccatcgagaa cgtctaccgg ctgcagctga
     6541 tgaacgccag cctgcgagcg cggcatctcg cggtgagcgc attcatcgac ggtggcgagg
     6601 cggtgccctt gcaggtgagc caggcgcgca gcttggtcat cgaacccgca ggcacaacga
     6661 cggccgcgct cactctgcat atggacgagc tcacggcaca gcgacaaggc ccgggccgcg
     6721 tggtcccgat ccgcatcgaa gtgcgcgacg ccgacggcgg cggtgcggtc cacgcggcat
     6781 cggcttccac tttcatgctc cactgacccg gcacgctgcg ctcagcgcag ggcctgacag
     6841 gccagctcgc ccagccggcg cagcgcggga tcgatgcgat cgtcacccgg gtgaccggca
     6901 ttcagccgga ggtggtgagc gaatcggcga tcggcagaga acaggactcc cggcgcggtg
     6961 ctgatgccta gcgacagggc ttgctggtgc agcgtcaagg catgcacggc agggggcagt
     7021 tccagccaga ggaaatagcc ccctgcgggc cgggtgacgc gcgtgcccga gggaaagtgc
     7081 cgctcgatca ggcgacgcac gcgctgctgc tccagggcga gcgtcgacct cagctgccgc
     7141 agatgccggt cgaagccgcc ctgggccagg tagtcggcga tcgccagttg cggcggtagc
     7201 gcggtcgcca gcgagctcat catcttcagg cgctgaaccg tgcgggcgta gcggccagcc
     7261 gccgcccagc cgacccggta gccgggtgcg aggcacttcg agaacgatga gcaatgcagc
     7321 accaggccct gcacgtcaaa cgccttggca ggtggcgggc gacgaagctc tgcatgcagt
     7381 tcgccgtaga cgtcgtcctc gatcaggggc acgccatgcc gtgccagcag cgtcaccagc
     7441 gcctgcttgc gcgctaccgg catcagggcc cccagcgggt tctggaagtt gggcatgaac
     7501 cagcaagcct tcaccggctg gcgggcgagc aactcggcca gggcagccag gtcaacccca
     7561 tcggtcggat cggtggccac ctccacggcc cgcaggttca gccgctcaag tgcctgcagc
     7621 gcagcgtaga aagtgggtga ctcgaccacc accaggtcgc ccggcgaggt gacggcctgc
     7681 aggcacaggt tgagcgcctc catcgcaccg ttggtgacca ccagttcctg ctcggccagg
     7741 gccacgccct gaagcgcata gcgccgccga agggcctgcc gcaagctctc gtcccccgct
     7801 gtcagcgcgc tggtgatcga ctgcggcttg acgcggcgca tcgcccgcgc tccgctgcgt
     7861 gcgagggcat cgaacggaaa caggtgggcg gcgggaaagg ccgacccaag tggcaccacg
     7921 gccgggtcgc gggtggactc cagcaggtcg aaggccagtt cgctgacgac cacgtccgtt
     7981 gcttccggtt tcggcacggc cggcagcggc agtgcccgcg gcgcgcggcg ctggttgcgt
     8041 acgaaatagc ccgagcgtgg ccgcgcctca atgagcccct gcagctccag caggccgtaa
     8101 gcctggaaca cggtcgacgg actcacacca cggctttgac aggtgtccct gaccgaaggt
     8161 agacgttcac ctggcttcaa caagccttcc ctgatcaact gcgtgacgtc gtgggccagt
     8221 tgctcatagc ggctggtgct gaggcctggc atctggtctg agtttgagga agtatcttca
     8281 gatcttacag atcagtttga aagaagcgat ggacgggaac cgaaactgcc agcgaggctt
     8341 gtcgtcgaac taaggcttgc gttccgcact gctgaccggt gggccggaca gccgacttcg
     8401 actgcttcca gccggaagtg agaaggggcc ccgcaggtcg ccttgctgtt aacgactgca
     8461 tcgatctgac ccacatgtcg ggaagggact ggaggctgcc agtcagtctc ggcggccgct
     8521 ggacgggacg agcgtgcgac cattcccgcg tgaacggccc attcaccccc cgccgcgcgg
     8581 tctgcgtgtg ctgcctgcgc ccgcagctga cctgcctgtg cgcgctcgcg cggccgacag
     8641 cccatcgcac cgaggtgctg gtgctgcagc atccgcagga acagcgccag gcgaagaaca
     8701 gcgtggcgat gctgcgcttg tctctggcgc attgcgaagt ggtcgtcggt gagcgcttcg
     8761 cgcctgaggt actggctgca ctgctgcagc gccccggacg ggacacgcgg ctgctctatc
     8821 ccgacgtgcc agccgcgccc gcgccgtcgg ccccggggac gactgcaggc gcgcccctgc
     8881 gtctggtggt catcgacgcg acgtggcgca agagcctgcg gatgcttctg gagcacccgg
     8941 ccctggctgc actgccgcgg ctgtcgctgg tcgcacccga gccgacctgc taccgcgcga
     9001 tccgcgcggc gcggcgagcc gaccaggtct cgacgctcga agccaccgtg caggcgctgg
     9061 cgatgctgga ggggccgtcc ttcgatgctg cgccgctgct cgacgccttc ggctgcttcg
     9121 tcgctggcga ggcggcccga cagccgcctc gggcgtcctc gggccaggcc tagtcagcca
     9181 cccgatggcc gggggaatgt cattgcccgg acgacgccga cagcagccag tcaagccgcc
     9241 gaggacggaa catctgctcg cttgaagcga tgacttcaac atgccgaccg caaagtggcc
     9301 ggcaagtggc cagtgaagca cggcctcaac gttgcgccta gggtgcctgc ctgtccgctg
     9361 cagtgattgg tcgggcgcaa tcacgctcag gaccgcttgc gcaatgtctt gcaggcaccg
     9421 atgcccaggc agatgagcgc cggccagacg gtgaccgcaa acagcaatcc gggaccgatc
     9481 gggttgggat tggggtcggg ccaaagaccg atggcggcca agaagatgat gagcaacaaa
     9541 ggcccccagc cgaacacgac gaaagccacg cccaccttgc tccagcggct ggatagaaat
     9601 cccttcatgc gatgcgctct ccttggcgct tgaccgtggg gaaggcagca ccgttcggac
     9661 tcctccacgg atctgtccaa cctcgttcgt tcgccgagcg tgccgtctcg gcgaagacaa
     9721 gcaatgggcc ggcatctgac gaccccatcg actggaatcg atctgaagcg catgtcagat
     9781 cgagcgaagg gttgggcgtc atttccacag ccgccaccat tgacgcttgg ccgcaagatc
     9841 tgtcagttgc tcgaatggtg tgtcgtcaac ctctggattg cactcgtcat ggagaaatcc
     9901 agtgatgtcc ttcgcgagga tgagtggaat gtccatgtga aagtgacctt caaaatcctc
     9961 gctgtctccg ggttcgaccg tggcgagcag ttcatgaaag cgcgccggca cggctccggc
    10021 atgagaaatg ttctcgacat ctcggtcgcc ttcatgttgg acatgccaaa cccgatgacc
    10081 gtcgtgccag agttccgtcg aggcgaagtt gacgtgctct tcgacagcac agaccaccgc
    10141 ccgacatccc accgagagcg ctgccatggt ccgggcacgt gatattcgat ggtcgcaggg
    10201 tgttgcagca accaggaacc agccgcccgg gagcgggtgg ctgcttaccc cgcggaatgg
    10261 gtagtcgcag tctctccccg ttcgcgacaa ggaaagccgt tgaagcgctt cttcgactga
    10321 aagcgcttcg actgcaaccc atgccagcga gattcccaag atgaaacttc gtgacgcctc
    10381 aaattcgagt caagcgggcg acggcgttct gccgttggcg cgctgcttga acgagcggtc
    10441 aggcctcact tggtggaccg ctctgcttgc tgaagcctgc gctccagttc gggctcgctt
    10501 tcgcgtgcga gctgcaccag cttgggtgca gacgagcgga gcgtctgctg gtgctttgct
    10561 tggaagaccc ggaaggccga agtcttgacc atggcgagga ccagagtgag ttcttcctcc
    10621 gagtaaacct ccatgtagga agcaatcttg gcctcggcat aggctcggga agcaatctgg
    10681 tcgctgagcc aactctcgac gattgccttg tgcttgcgca gcatgggatc ttgtgcaccc
    10741 atggctgatg cgacaagctg aattgcggtt gtggcgtcag cagcgacgag gaacttgtgg
    10801 agctcctgcg ctgccctgcg atgggcatct tgggcgaagg cggtcggcag catcgccgcc
    10861 caggcgacag cgatgaaaac ggcttttggg agatggaggt tcatgtgaga cccagggacg
    10921 acttgattca gcgtgcagtg ctgtacaacg ctggggttgc ggctgcacca ggcccggaca
    10981 gcacaccccg ggatattgac tgacccagcg aacccgacga tgggctgttg tttcatgcag
    11041 catcgagttc gtgtgaattc tgacgtccat cggcacgagc ccgccagcca cgccgccggt
    11101 gcaacggtgg gtcttcgacc cacccgcgag ggctgtcttg tacgtcaacg tgcggccaag
    11161 gccagacacc tgcacgcgca actgcatgcc tgtcatggag gctccgttcg cacagtcggc
    11221 cagggcatgc ataacccgct gcacgtcgtg ggctctatag attccggcct cgacggtcga
    11281 tatgcaccca tggaccgact tctctttcca gagtcagccg tggcaccgca agcacgtgtg
    11341 ctgcgcatgt ccgagctgct gggtgccctg agccgtgcgc tggacatcac cgaaggtcag
    11401 ccgccgggcc actgcgtgcg cagctgctgg ataggtatgc gcatcggcca ggtgctgggc
    11461 ctgcccggac tggcgttgtg ggagctgtac tacacgctgc tgctgaagga cctgggctgc
    11521 agcagcaatg ccgcgcgcat ctgtgagctc tatctcacgg acgaccgccg cttcaagcag
    11581 gacttcaaga cggtgggtga caacctgccg cagatgctgc acttcgtctt cacgcacacc
    11641 ggcttgaagg cgggcctggc gcagaggctg ggtgcggtgc tcaacatcat gcgcaatggc
    11701 cccgagattg ccaccgagct gatccagaca cgctgcacgc gcggcgccga cattgcacgc
    11761 cagctgcggt tcccgaccgc ggtgtgtgat ggcattgccg ggttggacga acactggaac
    11821 ggtcagggca agccacaagg cctgcgtggc aagcagatcc cgctgtactc gcgaatcgcg
    11881 ctgttggccc aggtggttga cgtgttccac tccactggcg gcccgcaggc cgcgctgatg
    11941 gaggtggccc ggcgcgccgg aacctggttc gatcccgagc tcgtgagggc gatcgcgagc
    12001 gtgggccagg acagtgcttt ctgggcgacg ctggccagcc ccggcttgga tcaggcggtg
    12061 caagcgctgg agccgggtgc ccatgtcgtg ccccttgatg agtcctacct tgacgacatt
    12121 gccgaagcgt ttggccaggt ggtggacgcc aagagtccct tcacgagtgg acacagcgta
    12181 cgtgtggcgg catacaccga gcgcatcgcc acgaaactgg gcctgtcgac tgcgcgcacc
    12241 cgctggctgc ggcgcggcgc gctgctgcac gacgtgggca agctgggcgt gagcaacagc
    12301 gtgctcgaca agccaggcaa gctggatgcc gaggaatggg gtgcggttca actccacgcg
    12361 gcctacacac aggacattct gggtcgcatt ggcgccttcg aggaactggc aggcatctct
    12421 gccgcgcacc acgagcggct ggacggcaag ggctatcccc tggggctggc cggcgaccag
    12481 atcagcatcg agacccgcat catcaccacc gccgacatct tcgacgcgat ctcggccgaa
    12541 cgaccctacc gcagcgcggt gcccattcca cgcacgctgg agatgatggc cgagaacctc
    12601 ggcacggcga tcgacccgct gtgcctggac gcactgaccg ccgtgttgct ccaggaaggg
    12661 caacttcacg gtggagtgaa ccactgaagc tggaccggca ggtaaagaca gactgatacg
    12721 ctggatgggc cttcaacagg ataatgttca ggtccaaatg aagtccttac cggcggcacc
    12781 cggcgcagtt caatctgcag ctgcgccagg agattcgcag cggcgggctc ggccgtcgcg
    12841 atgcgcggca caagtacagc ctctcggcca atctcatcca gctctggctc acgcaattcg
    12901 agtgaggaga gctcacgggc aaagagttcg aggcggcttc tttggtggac tacgaagccc
    12961 gcgcggcgcg ggtcgctggg cgggccagct ttctctgcta actgcgagcg gcaccgtcat
    13021 cgcgcctttc agcctgacat tctcatttcc gaagattgcg ttccgcacgg ttgaccggtg
    13081 tggccgtaca gccaattatc gctgcgtgcc gtcagaagcg aggaaggccc ccgcaggggc
    13141 cctggccatc atcccccccg acgccagccg tcgaacccca gtgcttgccg ctgcttggcc
    13201 caatccagtg gcaacggaga tgccgccagc atgcatttga tgttcacgcc cagcggcagc
    13261 gccccgctca tgatcttctg catgctgtcc ggcaccagga gcgccagatc agccacctgg
    13321 gtcacatcgg cattgagccc agctcagggc gcgttcacgt agtggcccgt caacatcatc
    13381 agtgccccgc ccgacatgga gtcggagaac acttgatagg tagcgcgggg gttctcgccg
    13441 gcaccgaagt aggccagcac gccgtgattg atgaagatgc gtgtgcccag cggcgtggca
    13501 ggcccacggg tgccggtaaa gggcagagtg gcataggcag acccgccgaa gctggactgc
    13561 aagaagccgt tgggcagggt accgttggcc gtatccacat atccggcgat ctgggtcacc
    13621 accaagcgct tgcctgctgg gaccgtgccg aacgagaagc tgcagacggt tgagccgcgg
    13681 cagcccaggt ccgacagcac ttcctggtaa tggttgcgcc ccggttcgtc caccgattga
    13741 accagtgcgg cgcgggtctg cgccagtgcg ggcgctgagc ccagcgcaaa gatggcaccg
    13801 gccgccaggg tcaacaagac cgggcagctt tgacgagggg tgaatgtcgg cttcatcatc
    13861 gttctccgtg ggtggtcagg ggcgggggca cgctatggcg gcggcgtcgg gcccaggcgg
    13921 ccgctaaagc tgctgtcaag accagcacgg cagagcccgg ttcaggcacg ataccgacga
    13981 catcaggcag cgtgatggca agtctgtatg aaccgtcgcc caagtacagt gggccaccca
    14041 ggccgatgaa gccatcggcc aggaacccgc cgctgttctc ggcgaaggac atgttctcga
    14101 acacgctgat ggccaccgtg tagtcgcctg cggtcagcac cacatcctgc gtgaaatcgc
    14161 cgcctacgct cactgcctgc aggtaattgg attcacggaa tgtggcgatg gcccggtcgg
    14221 tgccgctgaa gagtgtgaag tacgggtcga tcccgccagg aataaaacct gtgctggtga
    14281 agttgacgtt gccgcctacc gccacatgca gttcatacaa cgccacgttg tttgcggtgt
    14341 ctatgtcgtc accgaactgg gcagcagaca tgttggacgc taccaatgcg gcgttggcgg
    14401 ggtcagccag cgatccattc agggtgacca aaccggccag cacggggcct gcaaagacgg
    14461 ccagagccaa ggcggccact ggttggagac atctgtcgta catcgtcggc gctcccagcg
    14521 gcatctcttg caaaaagaga aagctgattc tgtgggtgtc tgtaccggaa tacaaccctg
    14581 agtgagcatt cggtccgggg gggcacttac cccgaacgac cgatttcgcg gttggtaatt
    14641 gctctgaatt gaccctgctg atcggctgtt ggaacgtccg cagcgcgaac tcactccgca
    14701 ccaggtcttc aatgcgtctt ccgcttgcag ttctgggtca gtggatgtga gttggtgggt
    14761 cagctcgatt aaatcgtcaa cagttcggcc actgtcacgc ctagggagta cctgcgcaac
    14821 tggtccctca cgctcgacct gcagatcatc ctgcgcacga tgcgcgtggc cttcatcgag
    14881 cgccatgcgt actgaagcgc accggtgtcg gtggtctgat ccgaggccga cgatcctgta
    14941 aaaacgggcc ttcgcggcct gtttttcatt ccggccgcga caggtggcta cgcaagtcaa
    15001 gcatgcatcc agcggttgct ggtttgacgg acgggccgtg tccagtgggt cgtcgacggg
    15061 agtggcagcc ggtgccatgc cgcgagcgtc gacatgcgag cagatcggat gaatcaagaa
    15121 cgcaacgcct catgcgcctg cgggtcagga cgaaagttca agaagtgctg cggagcgccg
    15181 gcgcctgttg ttgctgtcca gccgcagtcg gtgcgctcct gcggcggctg catgaagtgc
    15241 tgcgaggggt ggctggccgg accggtgcgc agggagggcg tggtggacca tgtgctgatg
    15301 cgaggttcgc catgtccgca tgcgacatcc ggccggtgtg ccatctacga cagccgtccg
    15361 gaagacccct gcaagtcttt cgtgtgcggt tggctgatgc cggcgagccc gctgcccgaa
    15421 agctatcgcc ccgacaagct cggcgtcata tttgttcagc ttgtctggcg gcagcgtcgg
    15481 gcgtgggcgc ttgttccggc cgggggcgac atcggcgagg aattgagggc gagcatgcgg
    15541 tcctacacgc aggcgacggg ggagccgcac ttgctcaagt accccgagcg cctgctgtgc
    15601 tatggatcgg cggagttcca gcaggacatg gtggcaaagg ccgagcgggg cgagaacccc
    15661 tgggaccctt gacgcctccg acgggttgct tgcagcggct gtgtcggtca agcttgcgtg
    15721 cgcttgcgta cagacacccc gatggcgtgg gcgatacaag gtccgagcga ctcgaacccg
    15781 cagtcaccac gtcacttcaa ggatgagcac atgaccaacg cacgcagaaa cttccacaag
    15841 accgcattca cgaccgcatc agccttgctg ctgggcacgc tgggcgtcaa cgcgagcgcg
    15901 gcgagcgccg aagacctcga caaggacgca gcgcaggccc tgctgacctt gtacaagtca
    15961 cgtccggtcg ccgaagtggt ttccaagaag gccaaggcga tcctggtgtt tcccaaggtc
    16021 ctgaaggccg gtctggtctt cggcggaagc tacggagagg gcgtgctgat gaagggctcg
    16081 cacgtcaccg attactacaa ctccgtctcg gcatcctggg gtctgcaggc cggagccgag
    16141 tcctatgcct atttcgtgtt cctgatgagc gacaaggccg tcaggtacct cgaaaaatcc
    16201 aagggctggg agctcggcgt cggaccgagt ctcgtgatcg tcaacgaagg cgtggcgaag
    16261 aatctatcga cctccacgct gaaggacgat gcctatgcat tcatctccga ccagcagggc
    16321 ctgatggcga gtctgagcat cgagggcacg aagatcagcc gcatcaagcg ctgatccagg
    16381 cgcggggccc ggtcgaagcg gctcccgcac ctgcccccca caagtcattc cggtcagggc
    16441 ggtcaactgc cctgaccggc atcgacttca ttcctcgcgc gaaccgaaga tgcccagcag
    16501 cgcgagcagc gactggaata cgttgtacag gctgaggtac acgcccaggg tggcggtgat
    16561 gtagttggtc tcgtgaccgt cctgcacgcg cttcaggtcg tacaggatga acgccgagaa
    16621 gataccgatg gccagcacgg ccagcgtgat catcagcgcg ctcgactgca ggaagatgtt
    16681 cgcgatgccg gccaccagca gcatgatcgc gccgatgaac aggaacttgc ccatcgaggt
    16741 gagatcgcgc ttgatcaccg aggacagcgt ggccatgccc aggaagatgg ccccggtgcc
    16801 ggcgaaggcg gtcatgatca ggctcgcgcc gttggccagg ccgagcaccg acccgaccag
    16861 ccgcgacagc atcaccccca tgaagaacgt gaaggccagg agcatcggga cgcccgcggc
    16921 cgaattcttg gtcttttcga tcgcgaacat gaagccgaag gcaccgacca tgaacacgat
    16981 caggccgatg cccggggaca tggcgcgtga caggccggtg gagatgccga tccaggcgcc
    17041 cagcacggtg ggcaccatcg acagggccag cagccagtag gtgttgcgca gaaccctgtt
    17101 gcgttgttca acggtcgcgg aactgccgcc gtaacccgag acaggggaag agtactgagt
    17161 gttcatggtg tttccttgag tgaagaggta gaagcaagtg aggttgtctg agaggccggg
    17221 tcggcctgca gggtccgggc ctggcgcttg ccgcgggcgc ggatgttgag tgcctccaca
    17281 gccagcgaga acgccatcgc ggcgtagatg tagcccttcg gaatcgccgc atcgaaggct
    17341 tcggcggtga gcgccgtgcc gaccatcacc aggaaggcca gcgccagcac cttgatcgac
    17401 ggatggcggt cgacgaactc gccgatcggc ttggcagcca ccatcatcac agcgaccgag
    17461 gtcaccacgg ccgccaccat cacgctgatc tggtcgacca tgccgacggc ggtgatgacc
    17521 gagtcgagcg agaacacgat gtcgatgatc gcgatctgcc caatgacgcc ccagaacagc
    17581 cggcggccgg cggcgtgcaa ctgctccgag tcggtggccg gcgggccctg ctgctcgctc
    17641 gcctcgactt cgacgaagat ctcatgcgag gccttccaga gcaggaacag gccaccgaac
    17701 agcagcacca ggtcgcggcc gctgatgccc tgaccgaaca ggttgaacag atcggtggtc
    17761 aggcccatca cccagctcag cgagaacagc agcagcagcc gcgagaccat cgcgaagccc
    17821 agcccgagac gacgcgcctg gtcgcgcagg tgcacgggca accgccccac caggatcgag
    17881 atgaagatga tgttgtccac gcccaggacg atttccaggg cggtgagcat cgcgaaggcg
    17941 atccacaggt tggggtcgga gagaaattcc atggcttgtg ctggtgccac cgctgcaccg
    18001 tgccgagcgg gcacgtgagg gggtcgaacg attcagggag ggcgcggacg tgccgcggcc
    18061 gggttcacgc gaggagggcg cgtgtgcggg gcggacgctg cggtccgtcc agatccggcg
    18121 acagccgcgt gggtgcggcg taggagtttg gtctggccat ggccagcagc ggtgtggtca
    18181 cctgcacgca gccggtcagg atggcgtgag ggtctgcggc ggcctcggcg tggcttggcg
    18241 cgggcaattc atcggggtgt tgcgtattcg tcgatgcacc cgccatccgc ttcaaggggt
    18301 cggctgtcca tggcgagtcg atttgttgtt cggcgttcgc cagcgcgagg gtacgggtct
    18361 gctccaggcc caggaaaccg gaccagcaga caaccagtgc gagaagcaac gcaacgaatt
    18421 tcgagctcat ttcccgacca ttttagttgg tgttcgaccc tgcgaggctg acgagtaacc
    18481 ctgcgagacg gctgcacatc ggtcggcggc gtggcccggc ggctatcgcc ggcagcacgg
    18541 ccgtcgacgg ctcaccgcta gttgacccag accgctgcag gggccgtgcc gtgtcggtgg
    18601 tgtgcgacat cagcctccgc gtaatcgtct gctccggcga cattgaactg ctgctggttg
    18661 atgcggcttg ctgcggcctc ggcttcgtct gccgggagcg gtcgtgagaa caggtagccc
    18721 tgcaggcgac ggcagccaat ctcgcgcagc atctggtact gctccagggt ctcgacgccc
    18781 tcgacaacgc agtccagtcc cagttcgatc gacaggcgca ccatcgtcga caggatcgtt
    18841 cgccctgccg ggctcaccgc atccaccagc agcgatcgat cgatcttcat cgagtgcaca
    18901 ggcaatctct gcaggtagct cagcgatgag tagcctgttc cgaagtcgtc gatcgagatg
    18961 cgcacgcctg catcgcggag ctcgcacagc tgcgtgatgc tgcgttcagg gttctccatc
    19021 agcaccgact cggtgacctc gaccgtgacg cgggaaggat cgatgcctgt gcctgcgatg
    19081 acggccatcg catcgttgct gaaattggaa tgggacaact ggacaaacga gacgttgacc
    19141 gcgatggact cgatcgtcaa gccgctcgag cgccacgcca tcaactgctt gcacgccttg
    19201 cgcaatgccc acagacccac cggcacgatc accccgctgc tctcgagcag cggaacgaat
    19261 tccccaggcg aaaccaggcc gaattccggg tgtcgccagc gcagcagcgc ttcgacgctg
    19321 tcgacgcgct cctgggtcag gttgaccacg ggctggtagt gcaattcgaa ttgctcctgc
    19381 aacagcgcct cccgcaggtc cctggtcagc ctgatcctgc gcatggcttg cggatcgacc
    19441 tcacgctgga acaggatgac cgcgctgtcg tcgactcgct tggcctggta catcgcctga
    19501 tccgcgcacc gcatcagttg tgcgacggtc tctccatggt cggggtagac cgccaccccc
    19561 atgctcgcgc gggcctggat ggtgcgcccg tccagcagca tcggaacagc cagcgaggag
    19621 agcagtcgct cggcgaccga gtgggcggct gcctcgcccg acatgccggg cagcacgatc
    19681 aggaattcat cgccacccag ccgaccgaag gaaggcgcca tgccgccgcg cccgggatcg
    19741 cctgtaccca ggatgacgtc tgtggatctc agctgcttct ggaagcggcg ggcgacctcg
    19801 atgagcagca ggtcgccgac gtggtggccg tgcacgtcgt tgaccgtctt gaagttgtcg
    19861 aggtccacca tcaccaccat cagtggcgat cgggccatgg ttgccgaccg cagtccggcg
    19921 tcgagcgttc ggtcgagctg gcggcggttg ggcaggccgg tcagggcgtc gtagtcggcc
    19981 tgctccttga ggagcgcggc attgcgacgc tgcgagatga agatcagtgc aatgacggcg
    20041 agtgtcgtca acgtaccgat cgcgatcagc gagcccacga tcaggtcgtg cctggcggtg
    20101 gcgtgaatgg cgtcgaatga cgcctgcggc acgtgtctcg acagaaccca cgaggagctc
    20161 atgggtcctt cgagtggatc ccggatggtc aggccggctt ttgtattgcc cgccaggatg
    20221 tcctgcagtc ccgcattgcg ccgatccgtc accgcgacgg agcttgcctc gtgcgtcagt
    20281 gcgtagtccg ccgcaggtgt cttgtcgagg cccgcacccc tggccagcga tcgttcttcc
    20341 ggtgtcaccg gaatgttcgg ccaaccgagc agccttgcct cgagcacatt gagccgaacc
    20401 acggtggtca ccaggccctt gaaggctccc gtttcctcgt catagatcgg caccgaaagc
    20461 atcatcccgg tgcgatcgcg ctgctggctt cccgaggttg aaaggaactg cgagttgtcg
    20521 caggtgatga tgggctccga tatcaggctg ggcatgccat gcggggcctg ggcgggcagt
    20581 gtcgggtggt ctcgcctgaa ctggttcagc tggtgaacga tctgcgcgta ttcctcctct
    20641 tcgacctgtt ccggcgcttc tccgagcagg tgtccatgtg tcgattcccg agccgtctgg
    20701 ctgaggtggc gaaagcggtc gacgatcacg gagtcgaaca tcaggaaggg gacttcgccc
    20761 cggtgcggct tgaagccgtc atagacgatg tagatctcgg acacggacat caggtccgca
    20821 aggtgcagat agagctgcgt cacggtctgt gcgtcgctgg tcgtgaagcg acgcccatcg
    20881 atcacgtcgt ccttgtccga tgcgcggttc tgcgctgcag gccggcgcac ggcgggcagc
    20941 atcgcgatcg tgcgcgtggt gacgtacagg tcggccagga cctgcgacag ctcgcgggac
    21001 ttggcctcgg tttcctgtcg cgccgtggat tccagcactt cgagggcatg gtcccgggtg
    21061 cgattgacgt cgctgacgat cagggactcc acgatcagca gcagcacgat ggcagccagc
    21121 gcgatgacga tgcgcggcga cggtggtcgg gtgttcgttc gcgccttgtg cagccaggtg
    21181 gcgatcatca gctgtccagt cgatggcttc gatgggtatt cgggtggtca ccaggaccgg
    21241 cttcagcgtc tatcgggtgt gaacgggaaa agttgatacc gaatcaaggc tttggcggcg
    21301 gaacgcggca acacgtcacg ccctgcggct gaagacgtcc gcttggctgt tgcgggcgca
    21361 tctggctgcg cgcactggtg cgctgtccgg gtttcatgaa aatctggagt tcggttcgcc
    21421 tccacggccg ctaggatccg gcgaggcccg cccattcgcg gcgtcaacct gccttttcat
    21481 gaaccgaatc gtcccgatga gaccgctgct gctcgcctgc ctgtgctgga tgctgtcttt
    21541 cgcctcgccg gcgtttgcga tgtcgccgcc cgatggcctg aacgatctgc ggccgatcga
    21601 tgaatcgcag tggacctcgg cttcggcggc ccacctgctg cagcgagccg gctttggcgg
    21661 gcgacccgat gagatcgcgg cgctgacccg gctcggcccg cgagaggcgg tgcgcaggct
    21721 ggtgcgctgg cagtccgtgc cggacaactt cgtgccgttc gacgagtcgg acctgcacga
    21781 cccgggtctg gaacccttcc cggcttcccg gcccgccacc accgatgcgg ccaagcgcga
    21841 tggtcaggcg atcggcgtcg ccgccaagcc cgccggcaac aaccgccggc tgcaaccggt
    21901 gacgaacaag ttcttctact ggctgcgcgc cagccgcctc gagacgcacc ggctgtcgta
    21961 ctggtgggcg cagcgcatgc tggtcacgcc gcgtccgctg caggagaagc tggcgctgtt
    22021 ctggcacggc catttcgcca ccagcgagga gaaggtacgc gactaccgca agatgctgcg
    22081 gcagaacatg ctgtttcgcg agaagggcaa cggcagcttc cgcgaactga tgatcggtgt
    22141 ggcgcaggac ccggccatgc tgtcattcct ggatgccggc gtgaacgtga agggcgcgcc
    22201 gaacgagaac ttcgcgcgcg agatcatgga gatgttcacg ctgggcgtgg gccagtacag
    22261 cgagcacgac atccgcgagg cggcccgcgc cttcaccggc tggaacgcga aggacctgac
    22321 gttcaccatc gatgcggcgc gccatgacga cggcatcaag aggttgctcg gccgcgaggg
    22381 accgctcgac ggcatcgacg tcatcgacac gctgctggcc cagcctgcgg cgggcgaatt
    22441 catcgccggc aagctctaca tgtacttcgt gcgacaggag gtgtcgcccg cgctgcgcac
    22501 cgagctcggc tcgcgcctgc gggccagcgg ctacgacatc gctgatctgc tggaaaccat
    22561 cttcatgtcc cgcgacttct acagccaggc gacggtggcc agccgcgtgc gctcgccggt
    22621 ggagctggtg gtggcgacct accggcaggc cgggctgacc caggttcctg gcgtgccgga
    22681 tttcaacgag gtcaccgatt cgctcggcca gaagctgttc tatccgccca ccgtggctgg
    22741 atgggcgcag ggccgcagct ggatcacgcc cggattgctc atcgcccgca gcaatttcgc
    22801 ctacgacctg gtctttccgg acatcaactt catcccaccc gaccgctacc cgtcggcaga
    22861 tttcaagatc cgggacttca gcgacaagct ggcactcggc ttcgacgtca ccaccgcctc
    22921 gaagctggaa ggcaccgaca tgagctcgat gtcgaaccag atggccgacc gggacgagga
    22981 gttcaacacg cgcctggcga gcttccgcgg atggcagatg gcgctggagc gtgtcaagcc
    23041 gattccgcgc gcgccggccc ggctcgagct cacgcgcctg gtgctgggcg acggttgccg
    23101 caccgccggc gacgcggtcg atgcgctgct gcggcgcttc gtctccgcgc cggttgcccc
    23161 ggccacccgc gcgcgcctga ccgagttcct gcagcaagag ctcggcaccc ccgacctggc
    23221 tgctgcgcgc agctacctcg atgagccgct gcggcagacg ctgcacctga tcctttcgtt
    23281 gcccgaatac cagctcggct gagcgcgcca tgaacaagtc aaatcctgtc gatgggttgc
    23341 tgagccgacg ctcggcgttg aaagccggac tgatcggtac cggctggctc ggcggcgttc
    23401 ccggcgtcct gcagcaggcc tgtgcggcaa cgtcggcacg cgatgacaag cgcatcctgg
    23461 tcgtgctcga gctgtccggt ggcaacgacg gactcaacac cctggttccc tacggcgacg
    23521 acgcctacta ccggcaccgg cccaagctcg ggatccgtcg tcagcagttg cgggtgatcg
    23581 acgaccacca cggattcagc cccggcatgg ccggcttcga gcgcctctac aaggacggcc
    23641 gcatggccgt ggtccacggc tgcggctaca cgcagccctc gttctcgcac ttcacttccg
    23701 cagcctactg gcacaccgcg atgcccaaca gcggggagga atacggctgg atcggtcgcc
    23761 tggccgatgt gatcgatccg aagctcacgc ccaacttcgt cgtcaatatc gacgagaccc
    23821 agacgctcgc ggtgcgtgcg cgcaaccacc tgccggtgtc gttcgacgat cccaatcggt
    23881 tcgtccgcaa gggtctgtac gaagagcgcg cggtgctcga cgcggcgcct gtcgtgcagc
    23941 cgcaggattc ggcgagcctg cgcttcatgc gcgacgtggc cgtcagcgcg cgcgagtcgt
    24001 cgcaaatcgt tcgcgaagcc tgtgcccgct acaagacgcc gatcgactac ggcatcagcc
    24061 cgctcgacct gcccaagatc gccgcgctga tcgcagccga cctgccaaca cggctctact
    24121 actgcgccta ccggcgcaat gccttcgaca cccatgtgca gcaggccgaa ctccaccagc
    24181 ggctgctgac ctacgcatcg gacgcggtca gcgcgttcat gcgcgatctc gagcgcatcg
    24241 ggcggtcgaa ggacgtgacg ctgctggtgt tctcggaatt cggccggcgc gtgccggaga
    24301 acaccagcct cggcaccgat cacggcacgg ccaatcacat gtatgtgatc ggccagccgg
    24361 ttcgcggtgg tcattacggc acattgccca gcctgacgaa gctcgacgag ggcgacaacc
    24421 tgatccacac caccgacttc aggcgcgtct atgccacctt gatcgagggt tggctggggg
    24481 ccgatgccca gcaggtcctg cgcggccgct acgaggggtt tccgatgttc gggtgaacga
    24541 tcagtgtggc gccggaccgc tgaggaacaa gtcgagtttc gcgaagaact cgtcgggcac
    24601 ctcgaagtac ggcagggcac ccgtcttgaa cgtgtcgatt cgccagttgg agcgcgcctc
    24661 gacgatcctc tggttgcggt agtcggtgaa gtcgccctgc acgccgtggc tcatccatac
    24721 cggctgcgag atgccctcgt acacgcggtg gatgtcggcg ctgaacaggt ttcccgacag
    24781 gaagtgcagg ggggcgaagc gcgcgccagg ttgccgtgcg gtctgaacgt cgtaggccca
    24841 cagtttctcg tcgatgaccc ggcctcccca ggtccgctcc aggaaatagc gcaccaccgc
    24901 cggtcgggtc agcaggcgaa agagggattc ggaccacaac ggcacactga gcaaggcgtg
    24961 catgccagca atgccgcgcg tgctgccctc cgggccacgc ctggcctggt gaccgttcag
    25021 gccggtcggg ctgacgagtg ccaggcggtc ccattgatcg ggacgctcga cggccgctcg
    25081 agccaggaac tcggtgccca gcgagacggc cacaccggcg atcggcaacg ggccgcagcg
    25141 gcgacggatc tcagccacgg tcgaatgcag cgcatcggtc atcaggcgcg gcgtgtagtc
    25201 gcgatcgctg cgatcgctga ggccgaagcc cggcaggtcg atcgcgaaca cggtgcgggt
    25261 gtatcggtag cggtcgaaca ggggtcgcat ctcggccgcg gaggctgctg cattcacgct
    25321 gtgtaccagc agcagcggcg gaccgtgacc tgcgacgtag cagttcaggc catcgaatgc
    25381 gatgcactcg cctgacaacg caggcgccag cgtggaagcc gacgcgtccg gtgatgccgg
    25441 ggtgggcttg gggtgcggcg tcagtccggc acgggatcta agttcggtgc tcatggcaaa
    25501 gtctgtggcg tcggaccgag accggaggta ggaagacaac cgttgcagct gtcggacgct
    25561 gccctgatgg cgggctcgat cattccgcaa aagtgtcgaa tgaggcgcga gtattgcagg
    25621 acgggtttgc tgctcctgtg gccggctggc gcatactcgc ggaccgaagg gttttttgct
    25681 tgtcctcctc ttttcagctc cggtggtctg atgtcatcgc tccctcaaac agaatctgaa
    25741 gcgtctgctg catcacattc cacggcaacc gtggcgcgca ggctgggtgt ttccattccg
    25801 acggtgcagc gctgggtcga tcagggccac ctgaaggcat ggaagaccgt gggggggcat
    25861 cgtcgcatcg atgctgccag cgtcgacctg ttcatccagc gacgcgagcc ccaggacctc
    25921 gctggatccc tgatccagga cgcatccatc gcgccgttcg tgctgatcgt cgatgacaac
    25981 ccggtggaca gggagcttct tgtcggcctg gttgaagagg ccttgcccgc agccgcgatc
    26041 accgcggtcg gcagcggctt cgagtgcctg atcgccgtcg gtcagagaac gcccgacgtt
    26101 ttgatcaccg acatcatgat gccgcacatg aatggctttg aaatgctgcg ccatgtggcg
    26161 acccgatgcg cggtgcggcc gaaggtcgtg atcgcggtgt cgggccgatc gccggagcag
    26221 ttcgccgagc tcggggaact gccggacgac gtgcagttcc tggccaagcc gctgaatgcc
    26281 gatgtgctga tcgacctgct gcgggcctgt gcagacgcct cggggctgcg ggctatcgcc
    26341 cgggcctgac ctgcaggccg cgcctgcgca gcgccggtcg ttaaggcggc cgcgcgtccc
    26401 tgctcattcc tggaagatca ctcgatcgtc aggcggttga accagcgctg accagcgggt
    26461 atgtcatcgc gcagggccgg cctgtagggt ggcacgtcgg tcttgtccgg cggcgcgctg
    26521 atctggttca ggtcgatggc ttgcggctgg ccttccaggc cctgctcggt gagccgcaac
    26581 cggaaataga tgccgttcca gagccgggca ccgaattcgg ccggagtctt gtacatgaac
    26641 atcagcgagt gctccagcca ggcgaagtcg gacgcggtca ccgtgcccgg ttgcgggtag
    26701 gggtagggca catggcagag gatctctccg gacccctcca cgcacttgaa ctccttcatc
    26761 gacaggaaga agtccttgaa gttctcggtg gccgttgtca cgacgaaggc ggtgcggccg
    26821 tcggcctgcg gcgtgaactg caccgtgccg atcttcaccg cgtcgccgtt gcgggcatgc
    26881 agcgtcatcg tgcgcgttcc ggacagatcc caggcagagg ctgccagggg caggctggct
    26941 gcccaagtca ggagtgcaca ggcgctcagg tgtcggaaca agtgaatctg gagcatgggg
    27001 acagtcctcg gtggggcggc tcagcgctgc agaagatggc atctgcgccg aacacatgat
    27061 cgccgggacc gggctggtcc gtaacgagag atctccctcg aacgtgcggt gcagggaatc
    27121 gctggcgatg ccctgtcgcc caacgtcgac ccacgtcaga tcggacgggc ggcacggccc
    27181 cgcagttcat ggatttccgc ccctgtgccg tcgtccgcgc aacgaccgcc cgtcaacggt
    27241 atctcgagct tcttcatctt ctcgtagagc gtgctcttgg cgattcccag ttcgcgtgcc
    27301 accgccgtca tgttgccgct ctgccgcgag atcatggact ggagcgtttc acgctccgcc
    27361 tgcttgaggc ggttgagcga acttcccgga gcggatgcga cgctgtcccg ctgcgccagg
    27421 atccgcctgg gcaacctgat ctcgtccggc aaggcgtcct tgcgcaggac gttttcggat
    27481 gacatcagca aggctcgctc cagcacattg cgaagctcgc ggacgttgcc gggccaggcg
    27541 tactggaaca ggcactccag ggctctgggg tcgatgctcg gtcgcggaac ggtgtgctgc
    27601 gcggccagct tgtcgaggag gaactcggcg atcagttcga tgtcccctgg ccggctgcgc
    27661 agcgggggaa tgtcgatcga cgcgacggcg atccgataga acaggtccat gcggaagcgt
    27721 ccctcggaga cctcggcgcg caggtcgcgg ttggtggcgg cgatcagccg gaacttgacc
    27781 tttcgcggct tgacctcgcc cacccggtag atctcgcctt cctccaggac cctcagcaag
    27841 tggggttgca gatcgagggg catttcaccg acttcgtcga ggaagagtgt tccgccatcc
    27901 gcggcctcca gcttgcccat catcccgcca cgtcttgctc cggtgaacga cccttcggaa
    27961 taaccgaaca gctcgctggt caggagctcg cgagacagcc caccgcagtt caaggccacg
    28021 aagggcccgg cgctggatgc gctggtttcg tgtatggccc gcgcaaagat gtccttgccg
    28081 acgcccgttt cgccgagcag cagtatcggc acgctggatc gcgccagtcg ctgcgccgac
    28141 ccgatggtct cgagcaggtt gggcgccctg ccgatcactc tttcgaaagc cgcgttcttg
    28201 aggaacgaat gcttcgatcc agccgggttg ccgcgtcgtg tcggtgactg gggtgaaccg
    28261 cggtgcggaa tcgtgagcag cgagcccagc cactgatcct cctcgaagac cggttccagc
    28321 cagtcgcggc tgatccagtc gggcagatcg accggtggcg agttccgaat cggcgaggcg
    28381 atcttcaggt tggagaggtc cactgcactc ccgcctgccc gagcgttgct cagggcgcgc
    28441 agcgcgattt cagccgaatc ggatgccttg atcggaatgc cgtggcggtc caggacgatg
    28501 accccgtccg tccttgcgct ggtcagccgg cccatgcagc gctccagcaa gtggtagcgc
    28561 aactccagtt cacgccgtgc cagcctgctt tcgattcgac tggccgtcgt cacgaccagt
    28621 gccaggctgt gccggttgta ggtggagttg aggcccgaga cgtccacgac gcccaggatg
    28681 ctgttgtcga aaggatcgcg caccaccgtg gctgaacagg tccagcgctt gatgcctgcg
    28741 cagtagtgct cggcggaatg gatctgcacc ggctgcccga tggccagggc agtacctatg
    28801 gcattggtgc ccacggtggc ctcgctccag ctgctgccgg gtgacaggtg gatgcgctcc
    28861 gcggccccga aggtcgaggg gtcgccctcc agattcagca cgatgccctc gttggtcgtc
    28921 agcaccatga ccgttccggt ctggtcgagg aactgtcggg cgtgcgccat caccggggcc
    28981 ccggccgaca gcagctct
//
LOCUS       DAOORV010000030        28931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1879_length_28931_cov_10.552777, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000030 DAOORV010000000
VERSION     DAOORV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_28931_cov_10.552777"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PKV56_06045"
     CDS             complement(<1..171)
                     /locus_tag="PKV56_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOX67409.1"
                     /translation="MVKKSKSSRTPVTGTAAPGRINIGISEKDRAAIAAGLSHLLADT
                     YTLYLTTHNFHWN"
     gene            complement(275..1195)
                     /locus_tag="PKV56_06050"
     CDS             complement(275..1195)
                     /locus_tag="PKV56_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOX67410.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEEELD
                     VKLFERGSNEVSVTPLGSEIVRQAQTVIEQAAGIKEIARRGKDPVSGPLRLGVIYTIG
                     PYLLPDLVRQAIELVPRMPLMLQENFTVKLLDMLRTGELDAAILAEPFPDAGLAIAPL
                     YDEPFMVAVPKKHPLARRKRITAEELKKETMLLLGTGHCFRDHVLEVCPEYAHFSSDA
                     EGIRKSFEGSSLETIKYMVASGMGVTVVPQLSVPAEPDRHVTYVAFAPPVPTRRVVLA
                     WRRTFTRYEAIAALRNAIYACPLTGVTRLS"
     gene            complement(1269..3443)
                     /gene="recG"
                     /locus_tag="PKV56_06055"
     CDS             complement(1269..3443)
                     /gene="recG"
                     /locus_tag="PKV56_06055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOX67411.1"
                     /translation="MPTRAPTSKAARGPEPASAKSGPLRAMDKLGLRRDIDLALHVPL
                     RYEDETRLTAIADLHDGVTAQVEGVVQRCEIQLRNRRQLLVTVADDAGNTLLLRFLHF
                     YPSHQKTLAEGRRVRARGEARGGFFGLEMVHPTFKVVEGASPLATALTPVYPTSAALP
                     QAYLRRAVTSALQRADLAETVPADLLPRGLATLRDALQWLHHPPADVSLSALDDRAHP
                     AWQRLKFEELLAQQLSQLQAKRERARQRAPRFDVEAGGLHERLLAALPFTLTAAQRRV
                     VEEIAADLQRAHGDDDARTGATAVPMHRLLQGDVGSGKTVVAALAAAIAIHNGWQCAL
                     MAPTEILADQHFRKLVGWLEPLGIGTAWLTGSRKGRGRREMLERIASGEAGLVVGTHA
                     VIQDDVQFAKLGLAIVDEQHRFGVAQRLALRSKLEQGRLEPHLLMMSATPIPRTLAMT
                     MYADLDISTIDELPPGRTPIVTKVFADEKRDAVVARIRDEAAQGKQIYWVCPLIEEMP
                     ESDAEPVHDEARSTKRPFGERPDLQNVIDTHQRLTDALPDVGVGLLHGRMKASEKSAT
                     MARFTSGELSVLVATTVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRIGRGSVASV
                     CVLLYAAPLSLTGKARLKAMAETTDGFEIARRDLEIRGPGEFLGARQSGAALLRFADL
                     NEDLSLLHAARRAATSMLERHPEAARRQVIRWLGSRADYLKA"
     gene            3542..4648
                     /gene="queA"
                     /locus_tag="PKV56_06060"
     CDS             3542..4648
                     /gene="queA"
                     /locus_tag="PKV56_06060"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOX67412.1"
                     /translation="MTAARIASYTLADFDFELPPELIAQHPAAERSGSRLLDGTGAAP
                     VDRIFRDLPSLLDPGDLLVFNDTRVIKARLLGIKATGGHVEALVERVLPGNEVLMHLR
                     ASKSPKRGQRVRFARSVEAAMSADAQSGFDAEVLGRAGPEQSLFHLRFPSDPQALLER
                     HGHVPLPPYITHADDADDERRYQTVFAARPGAVAAPTAALHFDQALLDALTARGVEQA
                     RVTLHVGAGTFQPVRAENLADHRMHSEWFEVGAETVEAVARTRQHGGRVVAVGTTSLR
                     ALESAALDGELRAGVGDTDIFITPGFRFRVVDALVTNFHLPCSTLMMLVSALAGHAHV
                     MALYRHAIAERYRFFSYGDAMLLHATPEARTSRS"
     gene            complement(4652..6250)
                     /locus_tag="PKV56_06065"
     CDS             complement(4652..6250)
                     /locus_tag="PKV56_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOX67413.1"
                     /translation="MTNVSPLPGAPEASSPLSLPRWLLFAGIAALLLRCWLALALPIT
                     GDEAYFYWWGVYPELGYYDHPPMVGWLIAAMRATLGDTLWSIRLPVLLLPIALGGMLW
                     WALSAIDRTRAAWAVLLFWLAPINWLNVLITTDTPLIFWSVASACCLLVAERRTRLDS
                     RTWGLYALSGGLLGAALLSKYFAVVLGFAYFMYFVLFRRDRLAAFAVLLLCALPGPAI
                     NIAWNIDHCWSNIMFNLINRNAGEVFSWKKPATYVGMMFYLVSPVAAWMSWKHRADLG
                     TAFRAHRLLGCLVVVPLLFFALLSLKKIIGLHWVMSFYPFGFAFIAIGLPAHRLKACA
                     VGLAVLTGLHVVAVIGVSLMPMESWKSLSIYKGIVRSVKTAEMLKQVEAPGVVLMSNA
                     YTPASTYGYAMRQYMPVFGRGNFHARQDDLLVDYSVYQDRTIRVILTTRPRLEDYRPY
                     FGSVELLTYTQNQLEFYAVEGKGFKYEAYRDGVLAEINRRYYRIPAILPLAACPFCER
                     LCGHTRCAATDTPASQKPEAKEEP"
     gene            complement(6247..7110)
                     /locus_tag="PKV56_06070"
     CDS             complement(6247..7110)
                     /locus_tag="PKV56_06070"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOX67414.1"
                     /translation="MQLPPLLALMRPHQWLKNVFVFAGLVFSQSWHDAGRVTLVLSAF
                     GVFCLFSSAVYIVNDWRDRDSDALHPTKRLRPLASGAVGPGVAFALAALLAAGGVALA
                     NGNRTLLGLIALYVASNVAYSWWLKRVPVVDVFSIALGFMLRLLAGTVGVGIPPSRWL
                     ILTGIFAALFLGFSKRRAETFHATASQRSVLGHYPPALLDTFVAVTMTATLMTYSLFA
                     TSPESQAQHGERLVYTVPIVIFGMLRYAYQVHRGRGEDVARDLLRDPWIVGALAAWLL
                     VFIAPLLVASA"
     gene            7252..8385
                     /gene="tgt"
                     /locus_tag="PKV56_06075"
     CDS             7252..8385
                     /gene="tgt"
                     /locus_tag="PKV56_06075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOX67415.1"
                     /translation="MLTFDVLTTQGLARRGRMTLNHGVVETPIFMPVGTYGTVKGVMP
                     GALEEMGAQIILGNTFHLWMRPGLDVLEQFGGLHRFERWSKPILTDSGGFQVWSLGDT
                     RKISEEGVRFSSPVNGDKLFMTPEVSMQIQRTLNSDIVMQFDECTPYDTAGHITTEPE
                     ARRSMELSARWARRCVDEFARGENPNALFGIVQGGMFEGLRDESLASLVELNLPGYAI
                     GGLSVGEPKEDMLRVLQHTAPKLPATKPRYLMGVGTPEDLVDGVAAGIDMFDCVMPTR
                     NARNGHLFTRFGDLRLRNSRYKNDEAPIDPTCSCQACAGGFSRAYLHHLERCGEMLAP
                     MLASIHNLHYYLNLMREIREALDAGRFDAFVAQFKADRARGIG"
     gene            8410..8895
                     /locus_tag="PKV56_06080"
     CDS             8410..8895
                     /locus_tag="PKV56_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67416.1"
                     /translation="MIWLRRLLGALLALVILFEEWGWEPLRRLMARIARLPLLAALER
                     RIATLPPHAALVVFFLPALALLPVKLGALWLIGQGHKLFGVLLILGAKVVGTAVVAHL
                     FALTQPALMRLPWFARYYTRWSGWKAALLARVRTSWAWRAGRVVKRRVARQLHRFTGP
                     R"
     gene            8946..9506
                     /locus_tag="PKV56_06085"
     CDS             8946..9506
                     /locus_tag="PKV56_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX67417.1"
                     /translation="MFDSAALQADIARLALGERAALRRVYEATSAHLFGIALRILNRR
                     DLAEDVLQEAFVNVWHHAGSYQASQAQPMTWLISIVRNKALDHLRSNQRHVADSIDAD
                     EDPEAHQIADERPNPMQLMMQAAETMEIRTCMESLDASPRQCLALAYYHGMSQSEIAE
                     HITAPIGSVKAWVRRGLERLKKCLAH"
     gene            9535..10275
                     /locus_tag="PKV56_06090"
     CDS             9535..10275
                     /locus_tag="PKV56_06090"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOX67418.1"
                     /translation="MNYNHPELLDRLAARFALGTLGGPARRRFVRLLRELPAARTALV
                     GWEERLAGLASSVPHVTPPDRVWEAIDARTAPRAAAAASKSGWLARLFKPAMGFAFGA
                     AMTASVVMTNPALFVSLDRLAQEEQGLPQSYVGLLTDAGNQPTLLVSSTRQGTRVSVK
                     FLRPVAVPAGRVIQVWALPKDGAPFPLGVAAPADPPKSTTFEISGSSEKLLSTVPRLA
                     VSFEPAPVTGATPPTGEFVLSGFCVKLW"
     gene            10484..11356
                     /locus_tag="PKV56_06095"
     CDS             10484..11356
                     /locus_tag="PKV56_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67419.1"
                     /translation="MKATQITLALVAAFAAVAVQAQSLTKPGVEAGNIDARGTVNGAI
                     NQVGGGTLVLLNGRGGRDLAPGGGSIISGGQNVRVGGVAGIVQASRINASGTVNGAIT
                     QLRAGALEGGNEQLVSIGSVSNTTANNVTATGTVTGIVLQSGGSAASGFNLQALQVGG
                     VDDVKANNVNATGTLTGTASQVSLGALAGNTEQVAVIGGARRVDADNLTLNGTLRGAL
                     TQVGANVASGFNTQVINIGGAHNARARNVNANGTVNGVVNQIGGNVGVDGSVQSAVVG
                     GVQGATVSGNDEAK"
     gene            11406..12287
                     /locus_tag="PKV56_06100"
     CDS             11406..12287
                     /locus_tag="PKV56_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67420.1"
                     /translation="MIPTHTTVLTLLLAAALGSAHAQDRGVDASNITTRSTITGSLTQ
                     TAAGLPGQSTQQTIAIGGVSGKVNANDISATANVVGAITQATTGASRQNIGIGSVQGN
                     VDARNVTVNGTAIGAITQSGLGRIDQRIGIGSVQGTVNARNITATGVTGGLIAQTGSG
                     TSVTDKQLILVGGVSDVDGRNLTATGAVGGVVTQTSIGAIGGTNVQQLLVGSINDSQV
                     HNVTTNGAVLGFVTQVAGSGLAGKNLQVINVGSVNNSSIRDATTFVVVTGNLTQLGAS
                     AGNNNTQKMILGGIDGR"
     gene            12309..12653
                     /locus_tag="PKV56_06105"
     CDS             12309..12653
                     /locus_tag="PKV56_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67421.1"
                     /translation="MSLALIPSRLGLLALLGAFLTCQVQAGVPEVGDDINARIARAKI
                     QRGGVDGGTKVKPVDASQPAGRGGDVLGDGCNIAIGNVFEQAKPGFGTSARAQTTVVV
                     QGDVIMANNRCK"
     gene            12688..14307
                     /locus_tag="PKV56_06110"
     CDS             12688..14307
                     /locus_tag="PKV56_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOX67422.1"
                     /translation="MNPTFKQHGLIAALTLALVGCAAPPSRQQDVATAASSRISTTPQ
                     RNVTDFTDGLRCMDETFLRYGTRDVTVIMEDLSDSTKKVSAGTRDMMISAISDMTRRS
                     RAIQLNTFGQDANNVVAFLANAKQRGPFGAVPQFNIRGSVSQLDESVLRRQSDLGLAL
                     EPLLSAGASRSRQFNVLGFDVSMVQTSNLTLLPGVSSKNIVVIAKEGDALDSQATINK
                     IGINFSTSFQRTDGTAQALRNMVEMSAIELFGRLQKLPYWTCLGVGPDNADIKREMED
                     WFIGMERGGELVPYFQEQLRNRGFYDGPVDGQISTGLRDAIKAYRRASGFSEVDFVDL
                     AFFSDFLHRPFPPPPAKPFVADVVPPVQQAEVALPPVPPAAPVKSPATSAVAGKKTTP
                     DPVAPQVASGDRRLLLLPIKETFAVGEAVGFHVIPSTSGYLYCYHQDAGGAIRRIFPN
                     RFTRDPRVTANRALTLPGRAAMELRASGGGQESVACFTTPNEIYNSLPAALRWGDFDP
                     LRLQSFDEVAAVFTPITKGRLARAEFRIVTR"
     gene            complement(14326..15006)
                     /locus_tag="PKV56_06115"
     CDS             complement(14326..15006)
                     /locus_tag="PKV56_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOX67423.1"
                     /translation="MALPLSRAARVRVIPFAVFMGLLALRGGVPADGSWGIDPRWIYG
                     ITVVAVGSLLAAWWSEYGELSRQVLPSLRECLLGVGVGLVVFGLWINLAAPWMQLGQP
                     TATFTPLDAQGQPIWPLIIVRWVGAALIVPVMEELFWRSFLMRWFDNEIFEGVVPQLV
                     GLRAIVLSTFVFMLAHTLWLAAIVAGLAYAWLYRRTGKLWVPVIAHAVTNGLLGVWVV
                     MTGNWAFW"
     gene            complement(15060..16274)
                     /locus_tag="PKV56_06120"
     CDS             complement(15060..16274)
                     /locus_tag="PKV56_06120"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOX67424.1"
                     /translation="MNVKAASAFGPLAGLKVVELGQLIAGPFAAKTLADFGAEVIKIE
                     PPATDGKPGGDPLRQWRLLHNGTSVWWQVQSRNKQSVALDLKDPDSREIVRRLIDEAD
                     VVIENFKPGVIEAWGLSHDELSKTNPGLIMCRISGYGQTGPYRELPGFAAVAEAMGGL
                     RHLTAEPGRVPVRCGVSLGDTLAALHGVIGILMALQHRHATVSPQAPKGIGQVVDVAL
                     YESVFNCMESLLPEYSAFGAVRAPAGSALPGIAPSNTYACTDGWVLVAGNGDSIYRRL
                     MHAIDRDDLGLDPGLATNAGRVARVDEIDAAIGAWAQTHSVAEALDTLQAAQVPVGRV
                     YTAKDIAEDPHYRARGMIERITTADGLSLDVPGVVPKLSATPGRIDRLAPTLGQDTDA
                     VLHALGINPPNR"
     gene            complement(16271..17113)
                     /gene="rlmJ"
                     /locus_tag="PKV56_06125"
     CDS             complement(16271..17113)
                     /gene="rlmJ"
                     /locus_tag="PKV56_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HOX67425.1"
                     /translation="MLAYRHAFHAGNHADVLKHITLCLVLRYMNQKDKSYRLIDTHAG
                     AGGYSLEGQYAQKKGEYLQGVARLWDRDDLPPAVADYMALVRQFNPDGRLEQYPGSPA
                     FAQMMLRAQDQLRLYELHPTDHRILEAFLGKVKGAEVKDTDGFDGLKSQVPPSSRRAV
                     VLMDPSYEGHGDYSKVIASLREAITRFPEGVYMVWYPQVSKLEAAQLPRRLEGLAPKG
                     WLHARLTVQVPDSQGFGLAGSGMFILNPPYTLHDELLAVLPYLTEVLGQYDGANYLLE
                     QKAA"
     gene            17251..18354
                     /locus_tag="PKV56_06130"
     CDS             17251..18354
                     /locus_tag="PKV56_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOX67426.1"
                     /translation="MTSLFDPIQIGDIAAANRIVMAPLTRNRAAAHQVPTDLMATYYA
                     QRASAGLIIAEATQISAEGQGYLDTPGIYSPEQVAGWKKVTDAVHAAGGKIVLQLWHV
                     GRISHVSLLPGGMPPVSSTARVAQAKTFTKEGFVDVSTPRALRSDELPRLVSDYRHAA
                     RCAVQAGFDGVEVHSANGYLLEQFLRDSINDRTDAYGGSIENRARLLIEVMQAVVDEI
                     GAAHTGVRLSPVTPFNDADQDSNAQALFNHVLERLAPLKLAFLHVIEGSTGGPRDNVP
                     FDYEALRARYKQGHPQGAWVLNNGYSRQMALDAVAQGLADAVAFGRPFISNPDLVQRL
                     RDDAPLNAMNPDTLYGGGAAGYTDYPTLAASAR"
     gene            complement(18418..18993)
                     /locus_tag="PKV56_06135"
     CDS             complement(18418..18993)
                     /locus_tag="PKV56_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOX67427.1"
                     /translation="MHLLSVNVSTARSITIDGREVLTAIGKRGVSGPVEARPLGLEGD
                     EQADQSVHGGLSKAIYAYPIEHYAFWQTVRAQAGVAGWNDTLPHGFMGENLTLDGLLE
                     NQVCVGDVLRFANCELAVSEPRFPCFKFNATMGFKHAAKLMVANAWCGYYLAVRTPGT
                     VSAGESFELIHGPREVGITELFRARRSAQRD"
     gene            complement(19055..19876)
                     /locus_tag="PKV56_06140"
     CDS             complement(19055..19876)
                     /locus_tag="PKV56_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67428.1"
                     /translation="MHNRRRALIRMSAAAAGIAAPALTPAVQRRSLDDPFRLAVDQAL
                     ADSGLAGRLQRGFGRDTGVAISVWPGPARELLAALERGEHDGALLNTPRAEEELDRLG
                     LLRDRRQIATADFLIVGPTLLRAGIDAMHARALAAQALSGLAQAGAPFVGATEGSGTR
                     ELEALLWRTAQVAPQAPWYRPSSPGQGALAAARANRACTLVERGVWAAAAGTMRPGKD
                     FGVLVEGDPLLRVPVHAMRSFRADHPAGRLFMKWLSSRTGRQAIAALPAYRPVSP"
     gene            20009..20557
                     /locus_tag="PKV56_06145"
     CDS             20009..20557
                     /locus_tag="PKV56_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOX67429.1"
                     /translation="MNRANPTRYTTTAIVLHWLLALAILGSFSLGLYMADLPFTPARV
                     KLYNWHKWAGVTILALSAFRLLWRLAHRPPPDVPMPAWQRHASHLTHRALYVLFFAVP
                     LVGWAYSSAAGFPIVWFGVLPLPDFVPVDKALAEAIKPFHGALAWALVAVVVLHVAGA
                     LKHQFVDRDGLISRMLPGPARG"
     gene            20598..21164
                     /locus_tag="PKV56_06150"
     CDS             20598..21164
                     /locus_tag="PKV56_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOX67430.1"
                     /translation="MNLFPVATLSMLAGLMASPVQAQQKVVPAQSEIGFVSRQMGVPV
                     DGKFRKFDAQVAFDPKQPAAAKIGFTIDLASASIGTAETEAELLKPDWFDTRKFPQAT
                     FTSTTVKPAGAGRFDVSGTLSIKGSSQSVLVPVTLAQAGATTTATGAFTLKRLDFKIG
                     AGDWGDTSIVANDVQVKFKLTLTGVAAL"
     gene            21227..21799
                     /locus_tag="PKV56_06155"
     CDS             21227..21799
                     /locus_tag="PKV56_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOX67431.1"
                     /translation="MKTSTVAVLAAALLASGLAQAESVTYSVEPTHTYVTFEVRHFGT
                     STNRGRFDKKEGSITIDRAAKTGQAEITIDMTSISTGLALFDKHLRGENFFKAAEFPT
                     ARFVGDKFTFDGDKVTAVSGTLTLLGKTQPVTLAATHFNCYENPFVKREVCGGDFETT
                     IQRSTYGMTYGLPGIPDSIKLLIQVEGVKQ"
     gene            22142..24121
                     /locus_tag="PKV56_06160"
     CDS             22142..24121
                     /locus_tag="PKV56_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HOX67432.1"
                     /translation="MRARNEVKPRHPLGGRQVRWATALMASGALITAAGAAAAATTVT
                     AAPKPQWVKVTDEGGAFNLADPKKVRFGVSGRWVQKSLAGTVACTSTFFGRDPAKGVA
                     KQCQVYKVVAAPAPAPAPAPSPAPSGPPAVDAAVRDAVRLATQASFGNTEALAAEIRT
                     QGASKWIASQMALATSRYTSGGNGTVHQNTSNTGFCDKPANAGPNCWRDNFSTIPLVW
                     DFYRNAISKPDQLRQRVAFALQQIVVVSGTEADGTYGFRSYHNTLLDNAFGNYRQVLK
                     KVALSPVMGDFLNNVNNDKSAPNENFARELLQLFAVGTCQLNTDGSLRGGACAPTYDN
                     DMVRSYAYALTGWTYPAGGATPWGCWPKGTNCRYYGGDMVPVEARHDTVARTLMSGVS
                     LADGHKAPAALEAVLDSLIAHPNTAPFIGKQLIQQLVSSNPSAAYVARVSAAFTSGKY
                     ASFGTGQRGDLAATVAAVLLDPEARSSTVIVNGGKLRDPVLMFTSVLRGLNGQSDGDA
                     LSWWWGEELREHVFRPPSVFSFYPPDYPVAGTALVGPSFGIHNANAALQRLNYLTYLL
                     DWGGSAAAAGVPNAIGTKVNLTAFTADAADAGKLVDRLSVIATGQPLPTATRAEIIKA
                     VSWWTVSTDKTNWQINRAKMAAYLVFATPSYQVQR"
     gene            24142..25581
                     /locus_tag="PKV56_06165"
     CDS             24142..25581
                     /locus_tag="PKV56_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOX67433.1"
                     /translation="MNHTTSFDARRRWLLQSGASCASALGVGSLGNLLLGARPAHAAD
                     YKALVCIFLYGGNDGLNTIVPSDAARHAQYSGVRGALAIPRDRLVALPGTEFGLHPSL
                     AALSAAAAAGHIAPVFNVGPLYAPLTKAQYRATPSGAALKPDGLFSHSDQQVLWETAT
                     THSQERTGWGGRASAVLGTVNPVISVGGSARFGLTTLQSPLVLPGPGSTFGAYGLQPD
                     DLKWAPMAARKAAIDLMYAQPQDAALADAYSRMQRDAFAMSQRLGALVKVEPGKAGSI
                     AAIDSAFASITVSGRISSGLGRQLYQVAKLIAGNATVQGDRQIFFAQAGGFDTHAGQI
                     AGSATEGEHARRLQEVGDALAAFYTAMKNLGLGQSVTAFTQSDFGRTFKPNSSNGTDH
                     AWGNQHLVVGGAVRGGATYGTYPQLVLGGDDDVGTESWEQHGRWIPTTSVDQYAATLL
                     GWFGASDAQLDAALPNLRNFGTRRRLGFV"
     gene            complement(25612..27126)
                     /gene="glpK"
                     /locus_tag="PKV56_06170"
     CDS             complement(25612..27126)
                     /gene="glpK"
                     /locus_tag="PKV56_06170"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOX67434.1"
                     /translation="MSGADHLLALDQGTSSSRSIVFDRQGHIVSMAQREFRQIFPRPG
                     WVEHDPLELWSTQLATAREALQSAGLTARDIASIGITNQRETTVVWNRRTGEPIHNAI
                     VWQDRRAEPTCIALRERGLEDLFRRRTGLVLDAYFSGTKLKWILDQVPGARTAAAAGE
                     LAFGTVDSWLMWHLTQGTADTPVHATDVSNASRTLMLNVHGNRWDDELLALLDIPREV
                     LPAVHPSSHVYGHTRADLFGAPIAIGGVAGDQQSALFGQACFKAGLAKNTYGTGCFML
                     MHTGTQFQTSTNGLITTSAAQPTDVPEFALEGSVFIGGAVVQWLRDGLQAIKGSAEVQ
                     TLAESVPDSGGVMFVPAFTGLGAPYWQAEARGAIVGLTRGSSVAHIARAALESIAFQS
                     AALLQAMSRDAVAAGGSPVAELRVDGGACVNNLLMQFQADLLGIPVVRPKVIETTALG
                     AAYLAGLSCGLFDGLDALAAQWQAERTFRPTLPRERAAELMARWERAVRQTVAP"
     gene            complement(27123..27890)
                     /locus_tag="PKV56_06175"
     CDS             complement(27123..27890)
                     /locus_tag="PKV56_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOX67435.1"
                     /translation="MTPNPRQSKVLDEVRARGSVTVDALAEQFGVTLQTVRRDVALLA
                     DAGLLARFHGGVRVAESTTENIAYRQRQQLNEQAKLRIARAVAQAVPEGCSLIINIGT
                     TTEAIARELLRHKGLRVITNNLNVAAILSDNPDCEVIVAGGVVRSRDRGIVGEVTVEF
                     IRQFKVDIGLIGISGIEADGTLRDFDYREVNVARAIMTHSRQVWLAADHSKFNRPAMV
                     ELARFSELDRLFTDTAPPAPFPALLSEAGVQCVTCAP"
     gene            28114..>28931
                     /gene="glpD"
                     /locus_tag="PKV56_06180"
     CDS             28114..>28931
                     /gene="glpD"
                     /locus_tag="PKV56_06180"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOX67436.1"
                     /translation="MDGPATLSAAGSPRSAYDVVVVGGGINGVGIARDLAGRGQKVLL
                     CEKDDLASHTSSSSTKLIHGGLRYLEYYEFSLVRKALAERELLLRSAPHIMWPLRFVM
                     PHDPGMRPVWMIRAGLFLYDHLARREVLAGSRTIDLRRHPAGAPLKRGLRKGFVYSDG
                     WVDDARLVVLNAIDAAERGARVLTRWRCVDARRGAEGWQLRLTSTAGETLEVGARALV
                     NAAGPWAAQFLSEHAHAPKPKSLRLVKGSHIVVHKLFDHDHAYIFQNPDKRIIFA"
BASE COUNT         4699 a        10031 c         9663 g         4538 t
ORIGIN      
        1 gttccagtgg aagttgtgcg tcgtcaggta cagcgtgtag gtgtcggcca gcaggtggct
       61 caggccggct gcaatcgccg cgcgatcctt ctcgctgatg ccgatgttga tccggccggg
      121 ggctgcggtt ccggtcaccg gggtgcggct cgatttggat ttcttgacca tcgaggtctc
      181 ctggtttgtg tgatgaaggt cagcacgcct ttcggtgctg cgtgacggaa aagagtctat
      241 acacgcaggg cagtccgcgt tcgaacccgg gcactcagga cagccgcgtc actcccgtca
      301 gcgggcaggc gtagatcgcg ttgcgcaacg cggcaatggc ctcgtagcgc gtgaaggtcc
      361 gccgccaggc aaggacgacg cggcgcgtgg gcaccggggg cgcgaacgcc acgtaggtca
      421 cgtgcctgtc cggctcggct ggcacgctca gttgcggcac caccgtgacg cccatccccg
      481 acgccaccat gtacttgatg gtttcgagcg atgaaccttc gaagctcttg cggatgcctt
      541 cggcgtcgct cgagaaatgt gcgtactcgg gacacacctc gagcacatgg tcgcggaagc
      601 agtgcccggt tcccagcagc agcatcgttt ccttcttcag ttcctcggcg gtgatgcgct
      661 tgcgccgcgc gagcgggtgc ttcttcggca ccgccaccat gaacggctcg tcgtagaggg
      721 gggcgatggc gaggcccgcg tcggggaacg gttcggcgag gatcgccgcg tcgagttctc
      781 cggtgcgcag catgtcgagc agcttcacgg tgaagttctc ctgcagcatc agcggcatcc
      841 gcggcaccag ttcgatggcc tgccgcacca ggtcgggcag caggtacggc ccgatcgtgt
      901 agatcacgcc cagccgcagc gggcccgaca ccgggtcctt gccgcgacgc gcgatctcct
      961 tgatgccggc ggcctgctcg atcaccgtct gcgcctgccg cacgatctcg ctgcccagcg
     1021 gcgtgacgct gacctcgttg ctgccgcgct cgaacagctt gacgtcgagc tcttcctcga
     1081 gcttcttgat cgccacgctg agcgtcggct gcgagacgaa acacgcttcg gcggcgcggc
     1141 cgaagtgccg ttcgcgggcc accgcgacga tgtatcggag ttcggtcagg gtcatggtgt
     1201 cgaggggctg gatcgcggat agcgctgcgg ggatgccgtc aatgtagcga acccgcgagc
     1261 cgtgcgcttc atgccttcag gtaatcggca cggctgccga gccaccggat cacctgtcga
     1321 cgggcggcct ccggatgccg ctccagcatc gaggtggccg ccctgcgtgc cgcatgcagc
     1381 agggacaggt cctcgttcag gtcggcgaag cgcagcagcg ccgcgcccga ctggcgcgcg
     1441 ccgagaaact cgccgggacc gcggatctcg aggtcgcgtc gggcgatctc gaagccgtcg
     1501 gtcgtctcgg ccattgcctt caagcgcgcc ttcccggtga gcgacagcgg tgccgcgtac
     1561 agcagcacgc agacgctcgc gaccgagccc ctgccgatgc ggccacgcag ttggtgaagc
     1621 tggctcagtc cgaagcgctc ggcgtgctcg atcaccatca ggctcgcgtt cggcacgtcg
     1681 acgccgacct cgatcaccgt ggtggcgacc agcaccgaca gctcgccgga cgtgaaccga
     1741 gccatcgtcg cggacttctc gctcgccttc atcctgccgt gcagcaggcc cacgccgacg
     1801 tcaggcagtg cgtcggtcag cctctggtgc gtgtcgatca cgttctgcag atcgggtcgt
     1861 tcgccgaagg gccgcttcgt cgagcgggct tcgtcatgca ccggctcggc atcgctctct
     1921 ggcatctcct cgatcagcgg acacacccag tagatctgct tgccctgggc tgcctcgtcg
     1981 cggatgcgcg cgaccaccgc gtctcgcttc tcgtcggcga acaccttggt gacgatcggc
     2041 gtgcggcctg gcggcagctc gtcgatcgtg ctgatgtcca ggtccgcgta catcgtcatc
     2101 gccagcgtgc gcgggatcgg ggtggcgctc atcatcagca ggtggggctc gagccggccc
     2161 tgctcgagct tgctgcgcag cgccagtctt tgcgccacgc cgaagcggtg ctgctcgtcg
     2221 acgatcgcca ggccgagctt cgcgaactgc acgtcatcct ggatcaccgc gtgcgtgccg
     2281 accaccaggc cggcctcgcc cgatgcgatg cgctccagca tctcgcgacg cccgcgcccc
     2341 ttgcgactgc ccgtcagcca ggcggtgccg atgcccagtg gttcgagcca gcccaccagc
     2401 ttgcggaaat gctggtcggc gaggatctcg gtcggcgcca tcagcgcgca ctgccagccg
     2461 ttgtggatcg cgatcgccgc cgccagcgcc gccaccacgg tcttgcccga gccgacgtcg
     2521 ccctgcagca ggcgatgcat cggcaccgcg gtggcgccgg tgcgtgcatc gtcatcgccg
     2581 tgcgcacgct gcaggtcggc cgcgatttcc tcgacgacac gccgctgggc cgcggtcagc
     2641 gtgaacggaa gcgccgcgag caacctctcg tgcaggccac cggcctcgac gtcgaagcgc
     2701 ggcgcgcgtt gccgggcacg ctcccgcttg gcctgcagct gcgacagctg ctgcgccagc
     2761 agttcctcga atttcagccg ttgccaggcg ggatgggcac ggtcatccag cgccgacagc
     2821 gacacgtcgg caggcgggtg atgaagccat tgcagcgcat cgcgcaacgt tgccagcccg
     2881 cgcggcaaca ggtcggccgg caccgtctcg gccagatcgg cgcgctgcag cgccgacgtc
     2941 accgcacgtc gcaagtacgc ctgcggcagt gcggcgctgg tcgggtacac cggcgtcagg
     3001 gcggtcgcga gtgggctggc gccctcgacc accttgaacg tcgggtgcac catctccagc
     3061 ccgaagaagc cgccgcgcgc ttcgccgcgc gcacgcacgc gccgcccctc ggccagcgtc
     3121 ttctggtgcg aagggtagaa gtgcaggaag cgcagcagca gcgtgttgcc cgcatcgtcg
     3181 gccacggtca ccagcaactg ccgtcggttt cgcagctgga tctcgcaacg ctgaaccaca
     3241 ccttcgacct gcgcggtgac gccgtcatgc aggtcggcga tcgcggtgag ccgcgtctcg
     3301 tcctcgtagc gcagcggcac gtgcagcgcc aggtcgatgt cgcggcgcag gccgagcttg
     3361 tccatcgcac gcagcggccc cgacttcgcc gatgcgggct cgggtcctcg ggcggctttc
     3421 gatgtcggcg cacgggtggg catcgatgaa ttctgaccgc tggccggccc accgcatgga
     3481 acaatccggc cgctcggttc ggtctgtccg acacgccagc cgacgccctc ttgcgcactc
     3541 catgaccgcc gcccgcatcg cctcctacac gctcgccgat ttcgatttcg aactgccgcc
     3601 cgaactgatc gcgcagcatc cggcggccga gcgcagtggc tcgagattgc tcgacggcac
     3661 tggcgctgcc ccggtggatc ggatctttcg cgacctgccc tcgttgctcg accccggcga
     3721 cctgctggtc ttcaacgaca cccgcgtgat caaggcacgg ctgctcggaa tcaaggcgac
     3781 gggcgggcac gtcgaggcgc tggtcgaacg ggtgctgccg ggaaacgagg tgctgatgca
     3841 tctgcgcgcc agcaagtcgc cgaagcgcgg ccagcgcgtg cgcttcgctc gcagcgtcga
     3901 ggcagcgatg tccgctgatg cgcagagcgg cttcgacgcc gaggtgctgg gccgtgcagg
     3961 gcccgagcag tcgctgtttc atctgcgctt cccgagcgac ccgcaggcgc tgctcgaacg
     4021 ccacggccac gtgccgctgc cgccctacat cacgcatgcc gacgacgccg acgacgagcg
     4081 ccgctatcag acggtgttcg cggcgaggcc gggtgcggtg gccgcgccga cggcagcgct
     4141 gcacttcgat caggcactgc tcgacgcgct gaccgcccgc ggcgtcgagc aggcccgcgt
     4201 caccttgcat gtgggcgccg gtaccttcca gccggtgcgc gccgagaacc tggcggatca
     4261 ccggatgcac agcgaatggt tcgaggtcgg agccgagacg gtcgaggcgg tggcccgcac
     4321 gcgccagcac ggcgggcgcg tcgtcgcggt cggcacgacg agcctgcgcg cactcgaatc
     4381 ggccgcgctc gacggtgagc tgcgtgccgg tgtcggtgac accgacatct tcatcacgcc
     4441 gggctttcgc tttcgcgtcg tcgatgcgct ggtcaccaat ttccatctgc cgtgcagcac
     4501 gctgatgatg ctggtcagcg cgctggcggg gcatgcgcat gtgatggcgc tctaccgcca
     4561 cgcgatcgcc gagcgatacc gcttcttcag ctacggcgat gcgatgctgc tgcacgcaac
     4621 gcccgaggcc cgcacctccc gcagctgacg ctcagggttc ttccttcgcc tcgggcttct
     4681 gcgacgcggg tgtgtctgtc gccgcgcagc gcgtgtggcc gcacagccgc tcgcagaacg
     4741 ggcaggcggc cagcggcagg atcgccggaa tgcggtagta gcgtcggttg atctccgcca
     4801 gcacgccgtc gcggtaggcc tcgtacttga agcccttgcc ctcgaccgcg tagaactcga
     4861 gctggttctg cgtgtaggtc agcaactcga ccgacccgaa gtagggccgg tagtcctcga
     4921 gcctgggccg ggtggtcagg atcacccgga tcgtccggtc ctggtagacg ctgtagtcga
     4981 ccagcaggtc gtcctgccgc gcatggaagt tgccgcggcc gaacaccggc atgtactggc
     5041 gcatcgcata gccataggtc gacgccggtg tgtaggcgtt cgacatcagc accacgccgg
     5101 gcgcctcgac ctgcttcagc atctcggccg tcttcacgct gcgcacgatg cccttgtaga
     5161 tcgacaacga tttccagctt tccatcggca tcagcgacac gccgatgacc gcgaccacgt
     5221 gcaggccggt cagcaccgcc agcccgacgg cgcaggcctt cagccgatgc gcaggcagcc
     5281 cgatcgcgat gaaggcgaag ccgaacggat agaacgacat cacccagtgc aggccgatga
     5341 tcttcttcag cgacagcagc gcgaaaaaca gcagcggcac cacgaccagg cagcccagca
     5401 ggcggtgagc ccggaacgcg gtgcccaggt cggcgcggtg cttccagctc atccaggccg
     5461 ccaccggcga caccaggtag aacatcatcc cgacgtaggt ggccggcttc ttccagctga
     5521 acacctctcc ggcgttgcgg ttgatcaggt tgaacatgat gttcgaccag cagtggtcga
     5581 tgttccaggc gatgttgatc gccggccccg gcagcgcgca cagcagcagc acggcaaacg
     5641 ccgcgagccg gtcgcgcctg aacagcacga agtacatgaa gtacgcgaaa cccagcacca
     5701 ccgcgaaata cttcgacagc agcgccgcgc cgagcagccc gccggacagc gcgtacaggc
     5761 cccaggtgcg gctgtcgagc ctcgtgcgtc gttcggccac cagcaggcag caggccgatg
     5821 cgaccgacca gaagatcagc ggcgtgtcgg tcgtgatcag cacgttgagc cagttgatcg
     5881 gcgccagcca gaacagcaga acggcccacg ccgcccgagt gcggtcgatg gccgacagcg
     5941 cccaccacag catgccgccc agggcgatcg gcagcagcag caccggcaga cggatcgacc
     6001 acagcgtgtc gccgagcgtc gcgcgcatcg cggcgatcag ccagccgacc atcggcgggt
     6061 ggtcgtagta gcccagctcg ggatagacac cccaccagta gaagtacgcc tcgtcgccgg
     6121 tgatgggcag ggccagcgcc agccagcagc gcagcagcag cgcagcgatg cccgcgaaca
     6181 gcagccagcg cggcagcgac agcggtgacg acgcttcagg cgcgccggga aggggcgaga
     6241 cgttcgtcat gcggaagcca ccagcagtgg cgcgatgaag accagcagcc aggccgccag
     6301 cgccccgacg atccacggat cgcgcaacag gtcgcgcgcc acgtcctcgc cgcgtccgcg
     6361 gtgcacctgg taggcatagc gcagcatccc gaagatcacg atgggcacgg tgtaaaccaa
     6421 ccgttctccg tgctgcgcct gcgactcggg gctggtcgcg aacaggctgt aggtcatcag
     6481 cgtggccgtc atcgtcaccg caacgaaggt atcgagcagc gcgggcggat agtgcccgag
     6541 caccgatcgc tggctcgccg tggcatggaa ggtctcggcg cggcgcttcg agaaccccag
     6601 gaacagtgcc gcgaagatgc cggtcaggat cagccaccgc gacggcggga tgccgacgcc
     6661 gaccgtgccc gccagcagcc gcagcatgaa gccgagcgcg atgctgaaca cgtcgaccac
     6721 cggcacgcgc ttgagccacc acgagtacgc cacgttgctg gccacgtaga gcgcgatcag
     6781 gccgagcagc gtgcggttgc cgttggcgag cgcgacacca cccgccgcca gcaatgcggc
     6841 cagcgcgaag gcgacgccgg ggccgaccgc tccgctggcc agcgggcgca ggcgcttggt
     6901 cgggtgcagc gcatcgctgt cgcgatcacg ccagtcgttg acgatgtaga ccgcgctcga
     6961 gaacaggcag aacacgccga aggccgacag caccagcgtc acgcgcccgg cgtcgtgcca
     7021 cgactggctg aacaccagtc ctgcgaacac gaagacattc ttcagccact ggtgaggccg
     7081 catcaaggcg aggagcggcg gcagttgcat gaggctgggg gctggccggt tgggcgcgtt
     7141 caggagaaga gaaacgcatg cgcgttgcgc gagcgcggcg ccgaggataa cgccgcaacg
     7201 tgttgcagtg gccgtggatc ggccggcgac accgcacgcg acaatcgacc gatgctgacc
     7261 ttcgacgtgc tcaccaccca gggactggcc cgccgcggcc gcatgacgct gaaccacggc
     7321 gtcgtcgaga caccgatctt catgcccgtg ggaacctacg gcaccgtcaa gggcgtgatg
     7381 ccgggcgcgc tcgaggagat gggcgcgcag atcatcctcg gcaacacctt ccacctgtgg
     7441 atgcgccccg ggctcgacgt gctggagcag ttcggcggcc tgcaccgctt cgaacggtgg
     7501 agcaaaccca ttctgacgga cagtggcggc tttcaggtct ggagcctggg cgacacccgc
     7561 aagatcagcg aggaaggcgt caggttctcg tccccggtca acggcgacaa gctgttcatg
     7621 acgcccgaag tcagcatgca gatccagcgc acgctgaatt ccgacatcgt gatgcagttc
     7681 gacgagtgca cgccgtacga cacggccggg cacatcacca ccgagcccga ggcgcgccgc
     7741 tcgatggagc tgagcgcgcg ctgggcgcgg cgctgcgtcg acgagttcgc gcgcggcgag
     7801 aacccgaatg cgctgttcgg catcgtgcag ggcggcatgt tcgagggtct gcgcgacgag
     7861 tcgctggcca gcctggtcga gctgaacctg cccggctacg cgatcggcgg cctgagcgtc
     7921 ggcgagccga aggaagacat gctgcgggtg ctgcagcaca ccgcgccgaa gctgccggcg
     7981 accaagccgc gctacctgat gggcgtcggc acgccggaag acctggtcga cggtgtagct
     8041 gccggcatcg acatgttcga ctgcgtgatg ccgacacgca acgcgcgcaa cggtcacctg
     8101 ttcacccgct tcggtgacct gcggctgcgc aactcgcgct acaagaacga cgaagccccg
     8161 atcgacccga cctgcagctg ccaggcctgt gccggcgggt tcagccgtgc ctacctgcac
     8221 cacctggaac gctgcggcga gatgctggcg ccgatgctcg ccagcatcca caacctgcac
     8281 tactacctga acctgatgcg cgagatccgc gaggcgctgg acgcgggtcg tttcgacgcg
     8341 ttcgtggcgc agttcaaggc ggaccgggcg cgcggcatcg gctagcgtgc tgcggcgttg
     8401 catcgagcga tgatctggct caggcgcctg ctgggcgcgc tgctcgcgct cgtcatcctc
     8461 ttcgaggagt ggggctggga accgctgcgg cggctgatgg cgcgcatcgc acggctgccg
     8521 ttgctggccg cgctggagcg tcgcatcgcc acgctgccac cgcatgccgc cctggtggtc
     8581 ttcttccttc cggcactggc cctgctgccg gtcaagctgg gcgcgttgtg gctgatcggg
     8641 caggggcaca agctgttcgg cgtgctgctg atcctgggcg ccaaggtcgt gggcaccgcc
     8701 gtggtcgcgc acctgtttgc gctgacccag cctgcgctga tgcgcctgcc ctggttcgcg
     8761 cgctattaca cgcggtggtc agggtggaag gccgcgctgc tcgcacgggt gcgcacttca
     8821 tgggcctggc gtgcagggcg cgtcgtcaag cgccgcgtcg ctcggcagct gcaccggttc
     8881 acgggcccgc gctgagcggg tcgatggcaa ggcgcgtcta gactcgcggc tgttccgcgc
     8941 agcccatgtt cgattcagcc gcactccaag ccgacatcgc ccgcctcgcg ctcggcgaac
     9001 gcgcagcatt gcggcgcgtc tacgaagcca ccagtgcgca tctgtttggc attgcgctcc
     9061 gtatcttgaa ccgacgcgac ctcgccgagg acgtgcttca ggaggctttc gtgaacgtat
     9121 ggcaccacgc cggcagctac caggccagcc aggcccagcc catgacctgg ctgatctcga
     9181 tcgtgcgcaa caaggcgctc gaccacctgc gcagcaacca gcgccacgtg gccgattcga
     9241 tcgatgccga cgaagacccc gaggcccacc agatcgccga cgagcgcccg aacccgatgc
     9301 agctgatgat gcaggcggcc gaaacgatgg agatacgcac ctgcatggaa tcgctcgatg
     9361 catcgccacg gcaatgcctg gcgctggcct actaccacgg catgtcgcag tccgagatcg
     9421 ccgagcacat caccgcgccg atcggcagcg tgaaggcctg ggtgcggcgc ggactcgagc
     9481 gcctcaagaa gtgcctcgcg cactagccgc gccgtcgatg ccgctgcgat caccatgaac
     9541 tacaaccatc ccgagctgct ggatcgactg gccgccaggt tcgcgctggg cacgctcggc
     9601 ggcccggccc gcaggcggtt cgtgcggctg ttgcgcgaac tgcccgcagc gcgcacggcc
     9661 ctggtgggct gggaggaacg gttggccggt ctggcgagtt cggtgccgca tgtgacgccg
     9721 ccggaccggg tctgggaagc gatcgatgcg cgcactgcgc cgcgtgcggc ggcagccgcg
     9781 tcgaagtccg gctggctggc gcggttgttc aagccggcga tgggctttgc cttcggtgcc
     9841 gcgatgacgg ccagcgtggt gatgacgaac ccggcgctct tcgtgtctct cgaccggctg
     9901 gcccaggagg aacagggtct gccgcagagc tacgttggac tgctcaccga tgccggcaac
     9961 cagccgacgc tgctggtcag ttcgacccgc cagggcacgc gcgtcagcgt caagttcctg
    10021 cggccggtcg cggtcccggc gggccgggtg atccaggtct gggcgctgcc gaaggacggc
    10081 gcgccgttcc cgctgggcgt ggctgcaccg gccgatccgc cgaagagcac cacattcgag
    10141 atctctggca gctccgagaa gctgttgtcc accgtgccca ggctggcggt gtctttcgag
    10201 cccgcgcccg tcaccggtgc gacgccgccg accggcgagt tcgtgctctc gggtttctgc
    10261 gtcaagctct ggtaggcccg ggccgcgtcc gcggcgacac cggctttccc gatctggcgg
    10321 gtgttttgcc cctccgcgcg accctgcgcc acccaaccag ggtttccact tagaaaaaag
    10381 ttttcgccga actgcctccg atcggcgcat ccgttccgta agtgggttca ggcggccacg
    10441 aggccaccgc aacccaagca acccaaccct tacggagatc atcatgaaag caacccagat
    10501 caccctcgct ctcgtcgccg ccttcgccgc tgtcgccgtg caggcgcagt cgctgaccaa
    10561 gccgggagtc gaggccggaa acatcgatgc ccgcggcacc gtcaacggcg cgatcaacca
    10621 agtcggcggg ggaactctcg ttctgctgaa cggccgcggc ggccgcgacc tcgcccctgg
    10681 cggcggcagc atcatcagcg gcggccagaa tgtgcgggtg ggtggcgtgg ccggcatcgt
    10741 ccaagccagt cgcatcaacg caagcggcac ggtcaatggc gcgatcacgc agttgcgtgc
    10801 cggcgcgctg gagggcggaa acgagcaact ggtctcgatc ggctcggtgt cgaacaccac
    10861 ggccaacaac gtcaccgcga caggcaccgt gaccggcatc gtgctgcagt cgggcggctc
    10921 tgcagcctcc gggttcaatc ttcaggcgtt gcaggtcggt ggcgtggacg acgtcaaggc
    10981 caacaacgtc aacgccaccg gcaccttgac cggcaccgcc tcgcaagtgt cgctgggcgc
    11041 gctggctggc aacaccgagc aagtcgcggt gatcggcggc gcacggcgcg tcgacgcaga
    11101 caacctcacg ttgaatggca cgctgcgcgg agctctgact caagtcggtg ccaatgtggc
    11161 ctcggggttc aacacgcagg tgatcaacat cggcggtgcc cacaacgcgc gagcgcgcaa
    11221 cgtcaacgcc aatggcactg tcaatggagt ggtgaaccag atcggcggca acgtcggcgt
    11281 ggatggcagt gttcagagcg cggtggtcgg cggcgtgcag ggcgcgacgg tcagcggcaa
    11341 cgacgaagcc aagtgatcgc gccgctcagc cgcacgtgac gcctgcatcg caccggagcc
    11401 cgaccatgat ccccacccac accaccgtcc tgacgctgct gctcgcagcc gctctcggca
    11461 gtgctcacgc ccaggaccgc ggcgtcgacg ccagcaacat caccacccgc agcaccatca
    11521 ccggcagcct cacgcagacg gcggcggggc tgccggggca gtcgacgcag cagacgatcg
    11581 cgatcggcgg ggtgtcgggc aaggtcaacg ccaacgacat cagcgcaacg gccaacgtcg
    11641 tcggcgcgat cacgcaggcc accaccggag ccagccgcca gaacatcggc atcggttcgg
    11701 tccagggcaa cgtggatgcg cgcaacgtca cggtgaatgg caccgccatc ggcgcgatca
    11761 cgcagagcgg gctgggtcgc atcgaccagc gcatcggcat cggctcggtg cagggcacgg
    11821 tcaacgcacg caacatcacc gccaccggtg tcaccggcgg gctgatcgcg cagaccggat
    11881 cgggcaccag cgtcaccgac aagcagctga tcctggtcgg cggcgtcagc gatgtcgacg
    11941 gacgcaacct gacggccacc ggtgccgtcg gtggcgtggt cacccagacc tcgatcggcg
    12001 cgatcggagg caccaacgtg cagcagctgc tggtgggcag catcaacgac tcgcaggtgc
    12061 acaacgtcac caccaacggc gcggtgctcg gcttcgtcac acaggtggcc ggcagcgggc
    12121 tggccgggaa aaacctccag gtcatcaacg tcggcagcgt caacaattca tcgattcgcg
    12181 atgcgacgac gttcgtggtg gtcaccggca acctcaccca gctcggcgcc tcggccggca
    12241 acaacaatac gcagaagatg atcctcggcg gtatcgacgg ccgctgaaac aatcgaagga
    12301 gaacgaccat gtcccttgca ctgatcccct cgcgtctcgg cctgctggcc ctgctcggtg
    12361 ccttcctgac ctgtcaggtg caggccggcg tgccggaagt cggcgacgac atcaacgccc
    12421 gcatcgcccg cgccaagatc cagcgcggcg gtgtcgacgg tggcaccaag gtcaagccgg
    12481 tcgacgcctc gcagcccgcg ggtcgcggcg gcgacgtgct cggcgacggc tgcaacatcg
    12541 ccatcggcaa cgtgttcgag caggccaagc ccggcttcgg cacctcggca cgcgcccaga
    12601 ccaccgtggt ggttcagggc gacgtgatca tggccaacaa ccgctgcaag taattcagcc
    12661 ccacccccag acgtcggagc gcgagcgatg aaccccacct tcaagcaaca cgggctgatc
    12721 gccgcgctga ccctggccct ggtcggctgc gccgcgccac cgagccgcca gcaggatgtc
    12781 gccaccgccg ccagcagccg catctcgacc acgccgcagc gcaacgtcac cgacttcacc
    12841 gatggcctgc gctgcatgga cgagaccttc ctgcgctacg gcacgcgcga tgtcaccgtg
    12901 atcatggaag acctgtccga ctcgaccaag aaggtcagcg ccggcacgcg cgacatgatg
    12961 atctcggcga tctccgacat gacccggcgc agccgcgcga tccagctgaa caccttcggc
    13021 caggacgcca acaacgtggt cgccttcctg gccaacgcca agcagcgcgg accgttcggc
    13081 gcggtgccgc agttcaacat ccggggttcg gtgtcgcagc tcgacgagag cgtgcttcgc
    13141 cggcagagcg atctgggcct ggcgctcgaa ccgctgctga gcgccggtgc ctcgcgcagc
    13201 cggcagttca acgtgctcgg cttcgacgtg agcatggtgc agacgagcaa cctcacgctg
    13261 ctgcccggcg tctcgtcgaa gaacatcgtc gtcattgcca aggaaggcga tgcgctcgac
    13321 tcgcaggcga ccatcaacaa gatcggtatc aatttcagca ccagcttcca gcgcactgac
    13381 ggcaccgcgc aggcgctgcg caacatggtc gagatgtccg cgatcgagct gttcggccgg
    13441 ctgcagaagc tgccgtactg gacctgcctg ggcgtgggtc ccgacaacgc cgacatcaag
    13501 cgcgagatgg aggactggtt catcggcatg gagcgcggcg gcgaactggt gccgtacttc
    13561 caggagcagt tgcgcaatcg cggcttctat gacggcccgg tcgacggcca gatctcgacc
    13621 gggctgcgcg atgccatcaa ggcctaccgt cgtgcctccg gcttctccga ggtcgatttc
    13681 gtcgacctgg cgttcttcag cgatttcctg caccggccgt tcccgccgcc gccggcgaag
    13741 ccgttcgtgg ctgacgtcgt gccgcccgtg cagcaggccg aagtggcgtt gccgcctgtg
    13801 ccgccggctg cacccgtgaa gtcaccggcg acttcggctg tcgcaggcaa gaagacaacg
    13861 cctgatcccg tcgcgccgca ggtggcatcc ggcgatcgtc gactgctgct gcttccgatc
    13921 aaggagacct tcgcggtggg cgaggcggtg ggcttccatg tgatcccgtc gaccagcggc
    13981 tacctctact gctaccacca ggacgccggc ggcgcgatcc gacgcatctt cccgaaccgt
    14041 ttcacgcgcg atccgcgggt caccgcgaac cgggcgctga cgttgccggg ccgcgccgcg
    14101 atggagttgc gcgcgtctgg tggcggtcag gaatcggtgg cctgcttcac cacgcccaac
    14161 gagatctaca actcgctgcc tgcggcgctg cgctggggcg atttcgatcc gctgaggctg
    14221 cagtccttcg acgaggtggc ggcggtgttc acgccgatca ccaagggcag gctggcgcgt
    14281 gcggagttcc ggatcgtcac gcgctgagcg cccggatcgc ccggctcacc agaacgccca
    14341 gttgccggtc atcaccaccc acaccccgag caggccgttg gtgaccgcgt gcgcgatcac
    14401 cggcacccac aacttgcccg tgcgccggta cagccacgcg taggcgagcc ccgccacgat
    14461 ggcggccagc cacagcgtgt gggcgagcat gaagacgaag gtcgacagca cgatcgcgcg
    14521 caggccgacg agctggggca cgacgccctc gaagatctcg ttgtcgaacc agcgcatcag
    14581 gaacgagcgc cagaacagtt cctccatcac cggcacgatc agggccgcgc cgacccaccg
    14641 cacgatgatc agcggccaga tcggctgtcc ctgcgcatcc agtggcgtga acgtcgccgt
    14701 cggctggccg agctgcatcc acggcgcggc caggttgatc cacagaccga acaccaccag
    14761 gccgactccg acgcccagca ggcattcgcg cagcgaaggc agaacctgcc gcgagagctc
    14821 gccgtactcg ctccaccagg ctgccagcag cgaaccgacc gcgaccacgg tgatgccgta
    14881 gatccagcgc gggtcgatgc cccacgagcc gtccgcgggc accccgccgc gcagcgccag
    14941 cagccccatg aagacggcga aggggatcac gcgcacgcgt gcggcgcggc tcagcggcag
    15001 ggccatgtcg aaagctcctc gggctcgggg gggtgtggtc gggcggcgcg cgccgctgct
    15061 cagcggttcg gcgggttgat gccgagcgcg tgcagcaccg cgtcggtgtc ctgccccagg
    15121 gtcggtgcga gccggtcgat gcgacccggc gtcgcgctga gcttgggcac cacgcccggc
    15181 acgtcgagcg acaggccgtc ggcggtggtg atgcgctcga tcatcccgcg cgcgcggtag
    15241 tgcgggtctt cggctatgtc cttcgcggtg tagacacgtc ccaccggaac ctgcgcagcc
    15301 tgcagcgtgt cgagcgcctc ggcgacgctg tgcgtctgcg cccaggcgcc gatcgccgcg
    15361 tcgatctcgt ccacgcgcgc gacgcgcccc gcattggtcg cgaggcccgg gtcgagtccg
    15421 aggtcgtcgc ggtcgatggc gtgcatcagg cgccgataga tcgagtcgcc gttgcctgcc
    15481 accagcaccc agccatcggt gcacgcatag gtgttgctcg gcgcgatgcc aggcagcgcc
    15541 gagccggccg gcgcgcgcac cgcgccgaag gcgctgtatt cgggcagcag gctttccatg
    15601 cagttgaaga ccgattcgta cagcgccacg tcgaccacct ggccgatgcc cttcggcgcc
    15661 tgcgggctca ccgtcgcatg ccggtgctgc agcgccatca ggatgccgat cacgccgtgc
    15721 agcgcggcca gcgtgtcgcc taggctgacg ccgcaacgca ccggaacccg accgggctcg
    15781 gccgtcaggt ggcgcaggcc gcccatcgct tcggccaccg ccgcgaagcc cggcagctcg
    15841 cggtacgggc ctgtctgccc gtagccgctg atgcggcaca tgatcaggcc cgggttggtc
    15901 ttcgacagct cgtcgtgact cagcccccac gcctctatca cgccgggctt gaagttctcg
    15961 atcacgacat cggcttcgtc gatcagtcgc ctgacgatct cgcgcgagtc cgggtccttc
    16021 aggtcgagtg cgacgctctg cttgttgcgc gactgcacct gccaccacac gctggtgccg
    16081 ttgtgcagca gccgccactg gcgcagcggg tcgccgcctg gcttgccgtc tgtggcgggc
    16141 ggctcgatct tgatgacctc ggcgccgaaa tcggccagcg tcttggcggc gaaggggccg
    16201 gcgatgagtt ggccgagttc gacgaccttg agcccggcca ggggtccgaa tgcagaggcg
    16261 gctttcacgt tcaggcggct ttctgttcca gcaggtagtt ggctccgtcg tactggccca
    16321 gcacctcggt caggtacggc agcaccgcga gcagctcgtc gtgcagcgtg tacggcgggt
    16381 tgaggatgaa catgccgctg cccgcgagcc cgaagccctg cgaatcgggc acctgcaccg
    16441 tcagtcgcgc gtgcagccag cccttcgggg ccagcccttc gaggcggcgc ggcagctggg
    16501 cggcctcgag cttgctgacc tgcggatacc agaccatgta gacgccttcc ggaaaccgcg
    16561 tgatggcctc gcgcaacgac gcgatgacct tcgagtagtc accgtggccc tcgtagctcg
    16621 ggtccatcag cacgacggcg cgtcgtgagg atggcggcac ctggctcttc agcccgtcga
    16681 agccgtcggt gtccttgacc tccgcgccct tcaccttgcc gaggaaggcc tcgaggatgc
    16741 ggtgatcggt cgggtgcagc tcgtacaggc gcagctggtc ctgcgcgcgc agcatcatct
    16801 gcgcaaacgc cggcgaaccg gggtactgct cgagccggcc gtcggggttg aactggcgca
    16861 ccagggccat gtagtcggcg accgccggag gcaggtcgtc gcggtcccac aggcgcgcga
    16921 cgccctgcag gtactcgccc ttcttctgcg cgtactggcc ttcgagcgaa tagccaccgg
    16981 caccggcgtg cgtgtcgatc aatcggtacg acttgtcctt ctggttcatg tagcgcagca
    17041 ccaggcacag cgtgatgtgc ttgagcacat cggcgtggtt gccggcgtga aaggcgtgac
    17101 ggtaagcgag catgcgggag tttcctgatc aatcgggcaa tgtagccgct gcccggaaac
    17161 gaggtgtgcg ctgacctccc gcagttgacc gctgtcaagc gcgggggtgt cctcgacccc
    17221 agaatcccgg tttcacctcg aaggattcgc atgaccagcc tgttcgaccc gatccagatc
    17281 ggcgacatcg ccgccgccaa ccgcatcgtg atggctccgc tgacccgcaa tcgcgcggcg
    17341 gcccaccagg taccgacgga cctgatggcc acctactacg cccagcgcgc cagcgccgga
    17401 ctgatcatcg ccgaggcaac acagatcagc gccgagggcc agggctacct cgacacaccg
    17461 gggatctact cgcccgagca ggtcgccggc tggaagaagg tgaccgacgc ggtgcacgcc
    17521 gcgggcggca agatcgtgct gcagctgtgg cacgtcgggc gcatctcgca tgtttcgctg
    17581 cttcctgggg gcatgccgcc ggtgtcttcc acggcgcggg tcgcccaggc caagacgttc
    17641 accaaggaag gcttcgtcga cgtgtcgacg ccgcgcgcgc tgcgcagcga cgagctgcct
    17701 cggctggtgt ccgattaccg ccatgcggcg cgctgcgcgg tgcaggccgg tttcgacggc
    17761 gtcgaggtcc acagcgccaa cggctacctg ctcgagcagt tcctgcgcga cagcatcaac
    17821 gaccggaccg atgcctacgg cggatcgatc gagaaccggg cccgtctgct gatcgaggtc
    17881 atgcaggcgg tcgtcgacga gatcggagcc gcgcacaccg gagtgcggct cagcccggtg
    17941 accccgttca acgatgccga tcaggacagc aacgcgcagg ccctcttcaa tcatgtgctg
    18001 gagcgccttg cgccgctgaa gctggcgttc ctgcacgtga tcgagggctc gaccggcggg
    18061 ccgcgtgaca acgtgccgtt cgactacgag gccctgcgtg cccgctacaa gcaggggcac
    18121 ccgcagggtg cctgggtgct caacaacggc tacagccgcc agatggcgct cgatgccgtg
    18181 gcgcagggcc tggccgacgc ggtggccttc ggtcggccgt tcatcagcaa tcccgacctg
    18241 gtgcagcgcc tgcgtgacga cgcgccgctg aacgcgatga acccggacac gctgtacggt
    18301 ggtggcgccg cgggctacac cgactacccg acgttggcgg cctcggcacg ctgatctcgg
    18361 tcgcgacggg gcggcaccgt gcctgcggtg ccgcccttgt tctttcgggc gccgggctca
    18421 gtcccgctgc gccgaccggc gcgcccggaa cagttcggtg atgcccacct cgcgcgggcc
    18481 gtggatgagc tcgaacgact cgccggccga caccgtgccg ggcgtgcgca cggccaggta
    18541 gtagccgcac caggcgttcg cgaccatcag cttggccgcg tgcttgaaac ccatcgtcgc
    18601 gttgaacttg aagcacggga agcggggctc gctgaccgcc agttcgcagt tcgcgaagcg
    18661 cagcacgtca cccacgcaga cctggttttc cagcagaccg tcgagggtca ggttctcgcc
    18721 catgaagccg tgcggcagcg tgtcgttcca gcccgcgacg ccagcctgcg cgcgcactgt
    18781 ctgccagaag gcgtagtgct cgatcgggta ggcgtagatg gccttgctca ggccgccgtg
    18841 caccgactga tcggcctgtt cgtcgccttc cagccccagc gggcgcgctt cgaccggtcc
    18901 gctgacgccg cgcttgccga tcgcggtcag gacctcccgg ccgtcgatgg tgatcgaccg
    18961 ggcggtggaa acgttgacgc tgagtaagtg catggtggga ggaagtgcag tggaggacag
    19021 atgtgcagat ggccaagtgg aaacgcgggc cggctcaggg cgataccggg cggtaggccg
    19081 gcagggcagc gatcgcctgt cgtcccgtcc ggctcgacag ccacttcatg aacagccggc
    19141 ccgccggatg gtcggcacga aacgaccgca tcgcgtgcac cgggacccgc agcagcgggt
    19201 cgccctcgac cagcacgccg aagtctttcc ccggtcgcat cgttccggcg gcagccgccc
    19261 agacgccgcg ctcgaccagc gtgcacgccc ggttggcacg cgccgcggcc agcgcgccct
    19321 gacccggtga cgatgggcgg taccacggcg cttgcggggc cacctgggcg gtgcgccaca
    19381 gcagggcctc gagctcacgc gtaccggagc cctcggttgc cccgacaaac ggtgcgccgg
    19441 cctgcgccag cccggacagg gcctgtgcag cgagcgcccg ggcgtgcatc gcgtcgattc
    19501 ccgcgcgaag cagcgtgggg ccgacgatca ggaagtcggc tgtcgcgatc tgtcttcgat
    19561 cgcgcagcag gccgagccga tcgagctctt cctccgcacg gggcgtgttc agcagcgccc
    19621 cgtcatgctc cccgcgctcg agtgcggcga gcagttcgcg cgccggaccc ggccacacac
    19681 tgatggccac gccggtgtca cgaccgaagc cgcgctgcag gcggccggcc agtcccgagt
    19741 cggcgagtgc ctgatcgacc gccagccgga aaggatcgtc cagcgaccgg cgctgcactg
    19801 caggtgtcag cgccggcgcg gcgatgccgg cagcggcagc cgacattcgt atcagggcgc
    19861 gacggcggtt gtgcatgcgt gaggagtcta ggcggggtgc atccaccgtt tttgaacggg
    19921 tgcatcaagt ccggtgaaac caattccgga aacgctcttc tacagtacat gcactgtgat
    19981 cagccacggc gtgctccgcg ccgatgccat gaaccgagcc aacccgaccc gatacaccac
    20041 caccgcgatc gtgctgcact ggctgctcgc gctggccatc ctgggttcgt tcagcctggg
    20101 cctgtacatg gcggacctgc cgttcactcc tgcgcgggtc aagctttaca actggcacaa
    20161 gtgggccggg gtcacgatcc tggcgctgtc ggctttcagg ctgctgtggc gcctggcgca
    20221 ccgtcctccg cccgatgtgc cgatgccggc gtggcagcga cacgcctcgc atctgacgca
    20281 ccgcgcgctc tatgtgctct tcttcgcggt tcctctggtc ggctgggcct acagctcggc
    20341 ggccgggttt cccatcgtgt ggttcggcgt gctcccgctg cccgatttcg tgccggtcga
    20401 caaggcgctg gccgaagcca tcaagccgtt tcacggcgcg ctcgcctggg cgctggtcgc
    20461 ggtcgtcgtg ctgcatgttg ccggcgcgct gaagcatcag ttcgtcgatc gggacgggct
    20521 gatctcgcgc atgctgccgg gccccgcgcg tggctgacgc tgcgctgccg agtcttcacc
    20581 tcaacggacc acctgtcatg aatctgtttc ctgtcgctac gctgtcgatg ctggccgggc
    20641 tgatggcctc gcccgtgcag gcgcagcaga aggtcgttcc cgcgcaaagc gagatcggct
    20701 tcgtctcccg ccagatgggc gttccggtgg acggcaagtt ccgcaagttc gacgcccagg
    20761 tggcgttcga tccgaagcag cccgccgccg cgaagatcgg cttcacgatc gacctggcca
    20821 gcgcgtccat cggcaccgcc gagaccgagg ccgagttgct caagccggac tggttcgaca
    20881 cacggaagtt tccgcaagcc accttcacgt cgaccaccgt gaagccggcc ggcgcaggca
    20941 gattcgacgt gtccggcacg ctgagcatca agggaagcag ccagtcggtc ttggttccgg
    21001 tgacgctggc gcaggccggt gccacgacga ccgcgaccgg tgccttcacc ttgaagcggc
    21061 tcgacttcaa gatcggcgcg ggcgactggg gcgacacttc gatcgtcgcc aacgacgtcc
    21121 aggtgaagtt caagttgacg ctgaccggcg tcgccgcgct gtgaacccgc acgcaggttt
    21181 cctttccccc gtttctcccc aacagcctcc agacaggatc cccttcatga agacttccac
    21241 cgtcgccgta ctcgccgctg ccctgcttgc cagcggactg gcacaggccg aaagcgtgac
    21301 ctacagcgtc gagcccacgc acacctacgt gactttcgag gtgcgccatt tcggcacctc
    21361 gaccaaccga ggccgcttcg acaagaagga aggctcgatc accatcgacc gtgcggccaa
    21421 gaccggccag gccgagatca ccatcgacat gacctcgatc agcacgggcc tggcgctttt
    21481 cgacaagcac ctgcggggcg agaacttctt caaggccgcc gaattcccga cggccaggtt
    21541 cgtcggtgac aagttcacct tcgacggcga caaggtcacg gccgtgtccg gaacgctgac
    21601 cctgctcggc aagacgcagc cggtcacgct ggcggccacc cacttcaact gctacgagaa
    21661 ccccttcgtg aagcgcgagg tgtgcggcgg cgacttcgag accacgatcc agcgcagcac
    21721 ctacggcatg acctacggcc tgccgggcat cccggacagc atcaagctgc tgatccaggt
    21781 cgaaggcgtc aagcagtaag cgcttgatgg ctcccccacc gggctccccg cccggtccgg
    21841 gtcgccccgt tcgcgggcga cccgtcgcag cggctcttcg ctgcagtgac tcctgccttc
    21901 catttccgcc cagccgaccg gcctgtgttg ccgactgcgc tcttctgtca gcagatgtca
    21961 gtgaggcctc ggcccggcat cgctcgtgca caggatgact ccgtagccaa agtgccggtt
    22021 gccgccgtga attgcgtcac gattcgtccg taacaggtgt tgcggggtga cgtttcaggg
    22081 ctttcatgct cccggacagt gacggacatc acaccttttg taagcagatg gaggagtcgc
    22141 gatgcgcgct cgaaacgaag tgaaaccgcg ccacccgctc ggcggccggc aggttcgatg
    22201 ggccaccgcg ctcatggcca gcggggcctt gatcacggct gcgggcgccg cggccgctgc
    22261 caccaccgtc actgccgcac ccaagccgca atgggtgaag gtgaccgacg aggggggcgc
    22321 cttcaatctg gccgacccca agaaggttcg tttcggcgtg tccggccggt gggtgcagaa
    22381 atcgctcgcc ggcaccgtcg cctgtaccag cacgttcttc gggcgcgacc cggcgaaggg
    22441 tgtggcgaag cagtgccagg tctacaaggt ggtagccgcc cctgctcctg cacccgcccc
    22501 cgcaccgagc ccggcgccgt cggggccacc ggcggtcgac gccgcagtcc gcgatgcagt
    22561 gcgcctcgcc acgcaggcca gcttcggcaa caccgaggca ctggctgcag agatccggac
    22621 ccagggcgcg tccaaatgga tcgcgtcgca gatggcgctg gccacctcgc gctacaccag
    22681 cggtggcaac ggcaccgtgc atcagaacac cagcaacacg ggcttctgtg acaagcccgc
    22741 caacgccggg cccaactgct ggcgcgacaa tttctcgacc atcccgctgg tctgggattt
    22801 ctatcgcaac gcgatctcga aacccgacca gctgcgccag cgcgtcgcct tcgcgttgca
    22861 gcagatcgtg gtcgtcagcg gcaccgaagc cgatggcacc tatggcttcc gcagctacca
    22921 caacaccctg ctcgacaacg ccttcggcaa ctaccgccag gtgctgaaga aggttgcgct
    22981 gtcgccagtg atgggcgact tcctgaacaa cgtcaacaac gacaagtcgg caccgaacga
    23041 gaactttgcc cgcgagctgc tgcagctgtt cgcggtgggc acctgccagc tgaacaccga
    23101 cggcagcctg cgcggtggtg cctgcgcacc cacctacgac aacgacatgg tgcgctcgta
    23161 tgcctacgcg ctgaccggct ggacctatcc ggcgggaggt gccacgccgt ggggttgctg
    23221 gcccaagggc accaactgcc gctactacgg cggcgacatg gtgccggtgg aggcgcggca
    23281 cgacacggtc gcccgcaccc tgatgtcagg ggtttctctg gcggatgggc acaaggcgcc
    23341 ggcggcactc gaggccgttc tcgacagcct gatcgcgcac ccgaacaccg ccccgttcat
    23401 cggcaagcag ctcattcagc aactcgtcag cagcaacccg tctgcggcct acgtggcgcg
    23461 cgtttcggcg gccttcacct cgggcaagta cgcgagcttc gggaccggac agcgcggcga
    23521 tctcgcggcc accgtggctg ccgttctgct tgatcccgaa gcgcgcagct ccaccgtcat
    23581 cgtcaacggc ggcaagctgc gcgacccggt gctgatgttc accagcgtgc tgcgcggcct
    23641 gaacggccag agcgacggcg atgcactgag ctggtggtgg ggcgaggaac tgcgcgagca
    23701 tgtcttccgt ccgccgtcgg tgttcagctt ctatccgccg gactacccgg tggcgggcac
    23761 ggcgctggtg ggcccgagct tcggcatcca caacgccaac gcggcgctgc agcggctgaa
    23821 ctacctgacc tacctgctcg actggggcgg ctccgcggcc gctgccggcg tgcccaacgc
    23881 gatcggcacc aaggtcaacc tgacggcatt cacagccgac gccgccgatg cgggcaagct
    23941 ggtcgatcgg ctgtcggtga tcgccaccgg tcagccattg ccaaccgcga cgcgcgccga
    24001 gatcatcaag gccgtgtcgt ggtggaccgt gtccaccgac aagaccaact ggcagatcaa
    24061 ccgcgccaag atggcggcct acctggtctt cgcgacgccg agctaccagg ttcagcgctg
    24121 agcgcctcga aaggaaaact catgaaccac acgacctcat tcgatgcgcg ccgacgctgg
    24181 ctgctgcaat cgggcgccag ttgcgccagt gccctcggcg tcggcagcct cggcaatctg
    24241 ctgctgggcg cgcggcccgc gcacgccgcc gactacaagg cgctggtgtg catcttcctg
    24301 tacggaggca acgatggcct gaacaccatc gtcccttcgg atgccgcccg ccatgcccag
    24361 tacagcggcg tgcgcggcgc gctggcgatt ccgcgcgaca ggctggtggc cttgcccggc
    24421 accgagttcg gcctgcatcc gtcgctggct gcgttgtccg cagccgcggc cgcgggccac
    24481 atcgcgccgg tgttcaacgt cggtccgctc tatgcgccgc tgaccaaggc ccagtaccgc
    24541 gcaacgccca gcggcgcggc gctgaagccc gatggcctgt tctctcattc cgaccagcag
    24601 gtgctgtggg agaccgcgac cacccattcg caggagcgca ccggctgggg cggtcgcgca
    24661 tcggcggtgc tgggtacggt gaacccggtg atctccgtcg gtggcagcgc ccgcttcggg
    24721 ctcaccacgc tgcagtcgcc gctggtgctg ccggggccgg gaagcacgtt cggggcttac
    24781 ggactgcagc ccgatgacct gaagtgggcg ccgatggccg cgcgcaaggc ggccatcgat
    24841 ctgatgtacg cgcaaccgca ggacgctgcg ctggccgacg cgtattcgcg catgcagcgc
    24901 gatgcattcg cgatgtcgca gcgcctggga gcgctggtca aggtcgagcc cggcaaggca
    24961 ggctccattg cggcgatcga ctcggccttc gcgtcgatca ccgtcagcgg gcgcatatcc
    25021 agcggcctcg gccggcagct gtaccaggtg gccaagctga tcgcgggcaa cgcgacggtg
    25081 cagggtgacc gccagatctt cttcgcgcag gcagggggct tcgacacgca cgccgggcag
    25141 atcgccggaa gcgccaccga aggcgagcac gcccggcgcc tgcaggaagt cggcgacgcg
    25201 ctcgcagcgt tctacaccgc gatgaagaac ctcggcctcg gccagtcggt caccgccttc
    25261 acgcaaagcg acttcggccg aacgttcaag cccaacagca gcaacggcac cgaccacgcc
    25321 tggggcaacc agcacctggt ggtcggtggc gcggtgcgcg gcggcgccac ctatggcacc
    25381 tacccgcaac tggtgctggg gggcgacgac gatgtcggca ccgagtcctg ggagcagcac
    25441 ggccgctgga taccgacgac ttcggtcgat cagtacgcgg ccaccttgct gggctggttc
    25501 ggtgccagcg acgcgcaact cgacgccgcg ctgcccaacc tgcgcaactt cggtacgcga
    25561 agacggctcg gcttcgtctg accggtcggc gggtcccggg cggtggcgct gctacggcgc
    25621 caccgtctgc cgcaccgcgc gctcccagcg ggccatcagt tcggcagctc gctcgcgcgg
    25681 cagcgtgggc ctgaaggtgc gctcggcctg ccattgcgcc gccagcgcat ccagcccgtc
    25741 gaacaggccg cagctcaggc ccgccaggta ggccgcgccg agcgctgtcg tttcgatcac
    25801 cttcggacgc accaccggaa tgcccagcag gtcggcctgg aactgcatca gcaggttgtt
    25861 gacgcaggcg ccaccgtcga cgcgaagttc ggccaccggt gagccgccgg cggccaccgc
    25921 gtcgcggctc atggcctgca gcagcgccgc gctctggaac gcgatgcttt ccagcgcggc
    25981 gcgcgcaatg tgcgcaaccg aactgccgcg cgtcagcccg acgatcgcgc cgcgcgcctc
    26041 ggcttgccag tacggcgcac cgaggccggt gaaggccggc acgaacatca cgccgccgct
    26101 gtcgggcacg ctctcggcga gcgtttgcac ttccgcgctg cccttgatcg cctgcagccc
    26161 gtcgcgcagc cactgcacca ctgcgccgcc gatgaagacg ctgccctcga gggcgaactc
    26221 ggggacgtcg gtgggctgcg ccgcgctggt cgtgatcagc ccgttggtgc tggtctggaa
    26281 ctgcgtgccg gtgtgcatca gcatgaagca tccggtgccg taggtgttct tcgcgagtcc
    26341 ggccttgaag cacgcctgcc cgaacaatgc gctctgctgg tcgcccgcga cgccgccgat
    26401 ggcgatgggt gcgccgaaca ggtcggcccg cgtgtggccg tagacgtggc tcgacggatg
    26461 caccgccggc agcacctcgc gcgggatgtc gagcagtgcg agcaactcgt cgtcccaccg
    26521 gttgccgtgc acattcagca tcaacgtgcg cgacgcgttg ctgacgtcgg tggcatgcac
    26581 cggcgtgtcc gccgtgcctt gcgtcaggtg ccacatcaac cagctgtcga cggtgccgaa
    26641 agccagctcg ccggccgccg ccgcagtgcg tgcaccgggc acctggtcga ggatccactt
    26701 cagcttggtg cccgagaaat aggcgtcgag caccagtccg gtccggcggc gaaacagatc
    26761 ctcgagcccg cgctcgcgca gcgcgatgca ggtcggttcg gcacgccggt cctgccagac
    26821 gatcgcgttg tggatgggct cgccggtgcg tcgattccac accaccgtgg tctcgcgctg
    26881 gttggtgatg ccgatcgacg cgatgtcgcg tgccgtcagc ccggcggact gcagtgcctc
    26941 gcgcgcggtg gcgagctgcg tcgaccacag ctcgagcgga tcgtgctcga cccagccggg
    27001 ccgcgggaag atctgccgga attcgcgctg cgccatcgac acgatgtgtc cctgccggtc
    27061 gaacacgatg ctgcgcgaac tcgacgtgcc ctggtcgagc gcgaggaggt ggtccgcgcc
    27121 gctcatggtg cgcaggtcac gcactgcacg ccggcctcgc tcagcagcgc cggaaacggg
    27181 gccggtgggg cggtgtcggt gaacaggcga tcgagttccg aaaaacgcgc cagctccacc
    27241 atggccgggc ggttgaactt gctgtgatcg gcggcgagcc acacctgccg cgagtgcgtc
    27301 atgatggccc gcgccacatt gacctcgcgg tagtcgaagt cgcgcagggt gccgtcggcc
    27361 tcgatgccgg agatgccgat cagcccgatg tcgaccttga actggcggat gaactcgacc
    27421 gtcacctcgc cgacgatgcc gcggtcgcgt gagcgaacga cgccgccggc gacgatcacc
    27481 tcgcagtccg ggttgtcgct caggatcgcc gccacgttga ggttgttggt gatcacgcgc
    27541 aggcccttgt ggcgcagcag ctcgcgggcg atcgcctcgg tggtggtgcc gatgttgatg
    27601 atcagcgaac agccctcggg cactgcctgc gccaccgcgc gcgcaatgcg cagcttggcc
    27661 tgctcgttga gctgctgacg ctggcggtag gcgatgttct cggtggtcga ttcggccacc
    27721 cgcacgccgc cgtgaaagcg cgccagcagg cccgcgtcgg ccagcagcgc gacatcccgg
    27781 cgcaccgtct gcaaggtcac tccgaactgc tcggccagtg cgtccaccgt cacggaacca
    27841 cgggcacgga cttcgtcgag gaccttcgat tggcgggggt tgggtgtcat gggtgagcgc
    27901 gagatgcggc aaggatccgc gaagcagctt atccaagacc accgattcgc ccatgctcga
    27961 tttcctgctt gcgcgctttt cgttttcggc ttaaaaagaa aaaagaagaa aacaaccgaa
    28021 cagagacgaa cattttttgc gttgacccgg caccgccgcg gtcgcgccca tctccctgga
    28081 gcttccttga cgcagtcgcc gaacctcggt cccatcgacg ggcccgccac gctgtccgcc
    28141 gccggttcgc cacggtcggc ttacgacgtg gtcgtggtcg gcgggggcat caacggcgtc
    28201 ggtatcgcgc gtgacctggc cggacgcggc cagaaggtgc tgctgtgcga gaaggacgat
    28261 ctcgcgtcgc acacctcgtc ttcgtcgacc aagctgatcc acgggggtct gcgctacctc
    28321 gagtactacg agttttcgct ggtgcgcaag gcgctggccg agcgggaact gctgctgcgc
    28381 agcgctccgc acatcatgtg gccgctgcga ttcgtgatgc cgcacgaccc cggcatgcga
    28441 ccggtctgga tgatccgggc aggcctgttc ctgtacgacc atctggcgcg gcgcgaagtg
    28501 ctggccggct cacgaaccat cgacctgcgc cggcaccctg ccggggcgcc cttgaagcgc
    28561 ggcttgcgca agggcttcgt ctattcggac ggatgggtcg atgacgcgag gctggtcgtg
    28621 ctgaacgcga tcgatgcggc cgagcgcggt gcccgcgtgc tgacccgctg gcgctgcgtc
    28681 gatgcacggc gcggggccga aggctggcaa ctgcggctga ccagcacggc cggcgagacg
    28741 ctggaagtcg gtgcgcgggc gctggtcaat gccgccgggc cgtgggcggc gcagttcctc
    28801 agcgagcatg ctcacgcacc caagccgaaa tcactgcgtc tggtcaaggg cagccacatc
    28861 gtcgtgcaca agctcttcga ccacgaccac gcctacatct tccagaaccc cgacaagcgc
    28921 atcatcttcg c
//
LOCUS       DAOORV010000031        28870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1886_length_28870_cov_8.913413, whole genome shotgun sequence.
ACCESSION   DAOORV010000031 DAOORV010000000
VERSION     DAOORV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28870
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_28870_cov_8.913413"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="PKV56_06185"
     CDS             <1..1530
                     /locus_tag="PKV56_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOX67437.1"
                     /translation="RERGHVLEGLAVALANIDEFIETIKASPTPPVAKAALMSKSWDS
                     SMVREMLSRAESEAPGGREAFRPEGLPRSYGLQADGLYRLSDDQAGEILQMRLQRLTG
                     LEQDKIIGEYKEVMTTIADLLNILATPERVTSIISDELAAIKQEFGQTKIGARRSLIE
                     HNAQDLGTEDLITPTDMVVTLSHTGYFKSQPLAEYGAQRRGGRGKQATQTKDDDWIEQ
                     LFIANTHDYILCFTNKGRVYWLKVWEVPQGSRNSRGKPIVNMFPLLPGEKVNVVLPLT
                     GEARTFPAERFVFMATAKGTVKKTSLDEFSNPRKAGIIAVDLDEGDFLIGAAITDGQH
                     DVMLFSDGGKAVRFAEQDLRPMGRNARGNKGMNLEPGQSVIAMLVAEDETQSVLTATE
                     HGYGKRTSIVEYTRHGRGTKGMIAIQQSDRNGKVVAATLVRPDDEIMLITDKGVLVRT
                     RVAEIRELGRATQGVTLIALDTGSTLSGLQRIVENDVSAEGTPDTTAGDDSGSPTTET
                     P"
     gene            1530..2177
                     /locus_tag="PKV56_06190"
     CDS             1530..2177
                     /locus_tag="PKV56_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOX67438.1"
                     /translation="MKRLFALTALCLALQAQAQSTPAPAAAPVSQAKKELVQKLLTLQ
                     QPVFETIARNVVERPAMQMMQAAAQALQTQVPAEKREATGKSIETDVRQFVEESAPVL
                     RERAVKLAPSTFGAVFEEKFTEDELKQLIAWTESPVNKKFKQTLPEVETGFVKKLIAE
                     AGPLLDPKLQALQQKVRATLVANAAPAPAAAASAAPATKPAPAPAAKPAAKAASK"
     gene            2185..3300
                     /gene="serC"
                     /locus_tag="PKV56_06195"
     CDS             2185..3300
                     /gene="serC"
                     /locus_tag="PKV56_06195"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOX67439.1"
                     /translation="MPRPFNFSAGPATLPAEVLEQAASEMLSWHGCGVSVMEMSHRGR
                     EFMSICEQAESDLRELLSVPPEFRILFMQGGGLGQNAIVPMNLIGRSSSRTADFVLTG
                     SWSIKSQAEARRYGEANIAASSATGRPTQLPDPSTWHISKDPAYLHLCSNETIDGIEF
                     QELPDLRALGCTAPLVIDCSSHVASRPIDWSRVGLAYAGAQKNIGPAGLTLVFVRDDL
                     LGQALPICPSVFNYRTVADQGSMFNTPPTYAIYIAGLVFQWLKRQTESGLTGIAAIEV
                     RNQAKAKLLYDCIDASRMYRNEVDPSCRSRMNVPFFLSDVSLTEPFLEQARDAGLLQL
                     KGHKSVGGLRASLYNAMPLEGVQALVNHMQAFEARHG"
     gene            3293..4399
                     /gene="pheA"
                     /locus_tag="PKV56_06200"
     CDS             3293..4399
                     /gene="pheA"
                     /locus_tag="PKV56_06200"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOX67440.1"
                     /translation="MADQPTAINPELLALRTQIDALDRDLLALLNQRAALALQVGEVK
                     KKEGSVAFRPEREAQVIDGLKAGNRGPLSSDSVAPIWREIMSACRALETPTRVAYLGP
                     AGTFSESAALGFFGSSIVKVPCSSIDEVFRATTAGAADFGVVPVENSTEGVVARSLDL
                     FLTTPLFIIGETSIFVRHNLLRRDNSLTGIEAVCAHPQALAQCHNWLGNHLPNAERRP
                     VSSNAEGARLASLDPRLAAVASDRAGSEFGLHIVAPAIQDDAHNRTRFAVVTHPQRHP
                     QPQASGHDCTSLVVSVANRPGAVYDMLMPLKTHGVSMSRFESRPARSGQWEYYFYIDV
                     QGHPSQPEVAQALKELRGVCAFFKLLGTYPIDVH"
     gene            4482..5366
                     /locus_tag="PKV56_06205"
     CDS             4482..5366
                     /locus_tag="PKV56_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOX67441.1"
                     /translation="MFNQLGVIGCGLMGGSFALALRRAGLVKRVVGYSKSPSTTEKAR
                     RMGVIDQAAESALLAVSGSDIVLMAVPVAASEATFRAIRNLIEPGVLLMDVGSTKRDV
                     VDAARRVLKDRLSNFVPAHPIAGKEVAGIEHADATLYQGRQVILTPLPQTQPELIQKA
                     TDAWSAVGSQVLRMTPENHDAAFAAISHLPHLLAFAYFNGVAGQPAGRDFLSLAGPGF
                     RDFTRIAASDPTVWRDILLANREELLKQSRQFRQALDAMEYVMTSGNAEALEELIRAA
                     STARAGWSMNSGKSTPQR"
     gene            5441..7459
                     /locus_tag="PKV56_06210"
     CDS             5441..7459
                     /locus_tag="PKV56_06210"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HOX67442.1"
                     /translation="MYAIPFIDIPPLLHAGGTVRLPGSKSISNRVLLLAGLSEGTTTI
                     HDLLDSDDTQVMLTALRTLGCRIEAEGDSLHITGLGGRLQAKEASLFLGNAGTAMRPL
                     TAALAMLAATQGGTFELSGVPRMHERPIADLIDALRPLGCRIDYLGQAGYPPLRLLPG
                     SGALDLAAPIRVRGDVSSQFLTALLLALPLLTQDQQATVEIDGELISKPYIEITLNLL
                     ARFGVEVQRDGWQRFIIPAGSHYRSPGAIHVEGDASSASYFIALGAIASIDAPLRIEG
                     VGTDSIQGDIRFVEAARAMGAQVTGGAGWIEVRRGRWPLAAIDLDCNHIPDAAMTLAV
                     MALYADGPTRLTNIASWRVKETDRIAAMAAELRKLGAVVVEGADFIEIAPPLHWSPAS
                     IHTYDDHRVAMCFSLAACNPLAVPPPSDPVAVRILDPKCVGKTFPDYFEALFSVVGTK
                     PEWIPVITVDGPTASGKGTVASALSERLGYHVLDSGVLYRATALAALRGGVDSDDEAG
                     LAALAEALDIGFEAGSIHLDGADVTAQLRDEEVGALASRISALPAVRQALLRLQFSFR
                     QLPGLVADGRDMGTVVFPDADLKIYLTASAEARARRRHQQLEASGVSSTLEGLLAGLR
                     ERDARDQNRAIAPLKPAADAVLLDNSALTIESTVEAVKSCWEQRRPFA"
     gene            7567..9288
                     /gene="rpsA"
                     /locus_tag="PKV56_06215"
     CDS             7567..9288
                     /gene="rpsA"
                     /locus_tag="PKV56_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOX67443.1"
                     /translation="MSQPQTAITEGGESFADMFNESLTRSEMRMGEVITAEVVRVEHS
                     FVVVNAGLKSEAYVPIEEFKNDHGELEVQVGDFVSVAIDAVENGYGDTILSRDKAKRL
                     ASWLSLETSLESGEFVTGTVNGKVKGGLTVLVNGIRAFLPGSLLDTRPVKDMSPYEGK
                     TMEFKVIKLDRKRNNVVLSRRAVVEASMGEERAKLLGTLAEGAIVTGVVKNITEYGAF
                     VDLGGIDGLLHITDMAWRRVRHPSEVVQVGQELQAKILKFDAEKNRVSLGIKQLGDDP
                     WHGVSRRYPQGTRLFGKVTNIADYGAFVEIEPGIEGLVHVSEMDWTNKNVAPAKIVSL
                     GDEVEVMVLEIDEDKRRISLGMKQCKANPWEEFSANVKRGDKVKGPVKSITDFGVFVG
                     LAAGIDGLVHLSDLSWHEPGEAAVRNFKKGQEVEAIVLAVDVERERISLGIKQLDSDP
                     FTTYTSVNDRGSIVTGKVKTVDARGAEVQLGDDVTGYLRASEISRDRVEDARNMLKEG
                     DEVNALIINVDRKTRSIQLSIKAKDNADNQEAMQRLSQTSERENAGTTSLGALLRAKL
                     DNQGGNP"
     gene            9330..9659
                     /locus_tag="PKV56_06220"
     CDS             9330..9659
                     /locus_tag="PKV56_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOX67444.1"
                     /translation="MTRSDLVSRLAERFSQLTHRDTESAVKIVLDAMSDALARGHRIE
                     IRGFGSFSINRRPPRVGRNPRSGEQVVVPEKLVPHFKPGKALREAVDMNLPADGPPPT
                     PAGTTRT"
     gene            9762..10070
                     /locus_tag="PKV56_06225"
     CDS             9762..10070
                     /locus_tag="PKV56_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOX67445.1"
                     /translation="MRVITWVFRVFVFFALFAFALNNKQEAGVNWFFGYEWRAPMVFI
                     VLAAFGLGCTFGVVAMVPSWWKHRRLAKRLPASPSDRDLHNPLAAAPPIPEHPPRDGL
                     "
     gene            10060..11217
                     /gene="lapB"
                     /locus_tag="PKV56_06230"
     CDS             10060..11217
                     /gene="lapB"
                     /locus_tag="PKV56_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HOX67446.1"
                     /translation="MDFDIQWVLLGLPVAFALGWLASRFDFRQLKREQRDAPKAYFKG
                     LNLLLNEQHDKAIDAFIEAVQQDPDTSDLHFALGNLFRRRGEYERAVRVHQHLLNRAD
                     LPPAERDRAQHGLAQDFMQAGLFDRAETAWRALEGTAYDTDARLALLTLHERSRDWRA
                     AVDDAQKLERGSTGSYASRIAHYWCELALEADAKQQPTDADDALRRAHEAAPQAARPM
                     VMAGRRLARQGRHAEALEVWGELLASHPSSFNLVAGDYAASAIACSAQDTARLRLLAL
                     HQKKPSIDLLNAIGALDTDPASRRDRIRTQLSLLPTLSAAQAVLHEHEASAVPLLPAD
                     LEAVNEVIGRAAKAGQRYRCAACGFEAQHHHWQCPGCLGWDTYPPQRLEEL"
     gene            11230..13020
                     /gene="msbA"
                     /locus_tag="PKV56_06235"
     CDS             11230..13020
                     /gene="msbA"
                     /locus_tag="PKV56_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOX67447.1"
                     /translation="MARSASNDLYLRLIKRIRPHWRTFALGILAMVGYAATETALPAL
                     LKELLDGSFVNRDPSSIHAMPILIVLLFLARGLTDFVHVATLNAVASKVVLELRTDMF
                     DKLLHLPTSHFDREPSAALMSKLTYNAQQVSPIITVSLITIVKDSLIVVGLLGYMLYL
                     NWQLSLAFFTVLPVIAYVIRSVSRRLRSLSHGQQSSMGTMSHVIDEVIGGHREIKIFA
                     GEAYESKRFHQVASALRRYTMKAVTTSAANGPIVQAIAVLALAVIIYYASIQSSQNQL
                     TVGGFVSFFSAMALLLAPLKRLSNVNEVLQRGLAAAASVFEVIDTPNEPDAGTRALGR
                     STGQLQFEGACFRYPQADRDALVDITLDIRPGESVALVGSSGSGKSTLMSAIPRFHAL
                     DRGAIRLDGIDIRELRLADLRANIALVTQHVVLFNDTVAANIAYGLKNTASEADIVAA
                     AEAAHAMDFIREMPQGLATPIGENGARLSGGQRQRISIARALLKDAPILLLDEATSAL
                     DTESERLVQIAIDNLKRGRTTLTIAHRLSTIANADRVVVMDQGRIAEVGTHAELLALD
                     GIYCRLHRMQFGTADAAPTATVAPVADALI"
     gene            13052..14149
                     /locus_tag="PKV56_06240"
     CDS             13052..14149
                     /locus_tag="PKV56_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOX67448.1"
                     /translation="MTETSDERPRILIVRLSAIGDVVMASGLIPALRDRWPHAHLAWL
                     VEPAAAPLLAHNPRLDELIVWPRGEWRELWRTHRYAALLQRFLALRSRLRAGRYDIAL
                     DTQGLLKSGVWAWLSHAPRRISLIGREGSQHLATERVHRPQETGVPRRLGSEYRVLAS
                     HLGAADEAFQPDLAIGAAAREAARAALRSGSAVITAGGRYAVLCPFTTRPQKHWFEDR
                     WVALAGRCHDDRLVPVLLGGPGDREAAARIAAGAPGLVNLTGALKLDESVAVIAEACA
                     LVGVDTGLTHMGSALHVPTVALFGSTCPYLDAGTPRSSVLYEKLACSPCRRNPTCNGR
                     FDCMRALDADRVHAEMRRVMALPAPGTQVHR"
     gene            14146..15171
                     /locus_tag="PKV56_06245"
     CDS             14146..15171
                     /locus_tag="PKV56_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HOX67449.1"
                     /translation="MSTTAVYFVASPLQLLAAQQIARTHEAGSRQVLVWYQPGVGTHV
                     RPADWDASSYMPWPRWKPLPGWFGRHRRLLANIDLVAALIGRCDTLHLHSAVFDTEAI
                     NYFLRALPARCGARTVRGRILPDGLISIRRYPLSPIRRWSPWFRKLRRLVSPELNYWL
                     FTGDRIGSDASFCDRIYVLSDWPHEYDPAKVVVLSPLSQRAPTDAGSVLPSRALVIGQ
                     PLVGAGLMGTADRDAVARQIEQWLTSREIAQVDYKGHPKDPQRELCQPGYRVLDLDEP
                     LEIWLAHTPYQAVVGVRSTALLFARQLCAAQTEVVAFGWEHTRFKSQQERDDMRAVFD
                     RVGVRQA"
     gene            15168..16430
                     /locus_tag="PKV56_06250"
     CDS             15168..16430
                     /locus_tag="PKV56_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOX67450.1"
                     /translation="MSPPTWRDRIPAINSSLLAALFFLIPTQIAPAYVLSALMLALWL
                     VEGRLQAKWQVLRVNPVFWIFQAYFCWYVVSLAWTADMTAGASMVTRHLFFPLAALYF
                     TVARREHTQRYLVAFAAGVLLCELLAGYNWLQLNLYPHWPASLRAAKDAMETAPFVDR
                     ILFGPTLAFAGYITAWQALVCRRAGQRGIAVAWAAACLATIGVLSFSGSRTGMLSFSL
                     MMAVLTLQALKRHRAWAALCAAIVLVGTAAGLYAMSDGTTRQRVLDGFAEARNLDAAV
                     NLSVPLRYTMAVNTLHIIAEQPWLGVGAGDFVSAYKDMNTRRSPAWNAPRNPHNQLLF
                     TVATTGVFGGLLLLAVWFAPPWLNRNLNDGLGALRVGLPVFFFTVCLAESYLWRTNTA
                     LLFALFSALLYGPVPTLHPPADLRPTPT"
     gene            16560..16907
                     /locus_tag="PKV56_06255"
     CDS             16560..16907
                     /locus_tag="PKV56_06255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX67451.1"
                     /translation="MQTTSSGLQFEDTIEGTGEEAVSGQHVSVHYTGWLYNGGVKGAK
                     FDSSKDRGDAFKFYLGAGQVIKGWDQGVPGMRVGGTRVLVIPPELGYGERGAGGVIPP
                     NATLMFEVELLDI"
     gene            16946..17902
                     /locus_tag="PKV56_06260"
     CDS             16946..17902
                     /locus_tag="PKV56_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOX67452.1"
                     /translation="MSIQYDITHTTVYRYREPVSFGEHRVMFRPRDSHDLRVLATDLQ
                     VSPEAIVRMIQDPHSNSVALVQPLTSALELRIVCSFSIEHAHTNNLELPVSGLAEFFP
                     FAYSTDERFDLEMYLRPHHEDPQGKLMDWARQFVRTDGPNRTRDMLVAMNEHIRNGLV
                     YQGREEEGTQSPLDTLALGSGTCRDFALLMMEAARRLGVATRFVSGYIYDPALDTATT
                     FDDAGEPLSTQGAGSTHAWLQAYLPGAGWVPFDPTNNLLGGSQLIRVGVARDPSLAAP
                     VSGSWYGAADAYLGMEASVQVRRVVSPAWQGTAATDAESLAV"
     gene            18054..18464
                     /locus_tag="PKV56_06265"
     CDS             18054..18464
                     /locus_tag="PKV56_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67453.1"
                     /translation="MGHLKTYIVEDSPVIRENLIAALEELVSVDIVGTAADENTAVQW
                     MSSNSQSCDLMIVDVFLAQGSGLGVLKAARLHDKPTKLVVLSNYATPDMRRKCIELGA
                     DRVFDKSNEIDALMAYCLRLSTGDSGNSAFSSLS"
     gene            complement(18498..19130)
                     /locus_tag="PKV56_06270"
     CDS             complement(18498..19130)
                     /locus_tag="PKV56_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67454.1"
                     /translation="MIRIAIVDDHAIVRTGLRQFLSEEIGLTVVAEAANGRDALDIVR
                     AGGVDVMVVDLSMPDQSGVEVLAAIKARAPDLPVLILSGFPEQHYATTMLKQGAAGYL
                     NKDCDPEEIAKAIRTVFRGRKYITSGVAELLADGLAGGGEKLPHELLSERELQVFLRL
                     AKGEAIGTMAISMSLSVKTVSTYRTRVMEKMKLESNSDLTYYALKAGLIQ"
     gene            19181..20530
                     /locus_tag="PKV56_06275"
     CDS             19181..20530
                     /locus_tag="PKV56_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HOX67455.1"
                     /translation="MSPPLIGRFWRSAYAFPVACVAAIVLLAFSEVSFRSASNSMERL
                     EAMARARTDIQSLWRQLVDAETGQRGYLLTQRNEYLKPYRESLERIDASLHSLTTYYR
                     GHPQGAPLMERLSGLSKDKISELATTLHLQDEGGGAWRDLLMTNIGQEKMESIRSTAQ
                     QLLALETQRVAEGRADVHRTLMINRVGVGLTTLLSLLALALYFRQSSALVRVQREQKQ
                     AVQVERDRLDAEVKSRTAQLTELAQHLQTAREDERSRLARELHDELGALLTAAKLDAA
                     RLKARLGTMSPEVAERLLHLNKLLNEGIALKRQIIENLRPSSLSNLGLVAALEIVTSE
                     FATASGLQVHAELEPVELSSSGELTAYRLVQEALTNIAKYAKASEVWVVLSQQGASAA
                     ISVRDNGCGFDTLTPREAAHGLLGMRYRVEAEGGHMTLSSQKGHGTLIEARLPLRPS"
     gene            20793..20954
                     /locus_tag="PKV56_06280"
     CDS             20793..20954
                     /locus_tag="PKV56_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOX67456.1"
                     /translation="MLHYAVVFFVIALVAALFGFGGIAASAVGVAKVLFVIFLVLAII
                     SFLFGQMRK"
     gene            21019..21384
                     /locus_tag="PKV56_06285"
     CDS             21019..21384
                     /locus_tag="PKV56_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67457.1"
                     /translation="MRVTMPSQFVAGAASGAADAVDQIDRAEESHRLLAEEAHDAVDA
                     AVPVIDRLVKNAESLARRGIDGARDGSQQLRDRAVRATDSTVNYIKDEPLKAMLIAAA
                     TGAALMALMALLNRSRQSD"
     gene            21456..21812
                     /locus_tag="PKV56_06290"
     CDS             21456..21812
                     /locus_tag="PKV56_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67458.1"
                     /translation="MAAHPTVLTDHVEAYASLAVQELGTAATQWRSRALKTGIAMLCA
                     ASAAVLAGVSLMLWATTAPTDLQAPWALWIAPGLPALAAMACWWSAQEKPAEGPFAIL
                     RGQFAEDMALLREVNA"
     gene            21845..22408
                     /locus_tag="PKV56_06295"
     CDS             21845..22408
                     /locus_tag="PKV56_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67459.1"
                     /translation="MADAGTAPVDAVARLGQSRLRLRQHLIALNGNQAGPAGSQALTG
                     WLGSLRSIPLLGTALDTVSGWWSRHPLRVVATVLAASTASAVRPVAQRHPAWSVFGAL
                     AVGGLVMWAKPWRIALLRRVLYAGLLPQLVSKAMSQQPTTGWLSLVESVLRRSPTPAP
                     SAGPVTAMVIHPNLPAGSDFTRTSPLR"
     gene            22461..22775
                     /locus_tag="PKV56_06300"
     CDS             22461..22775
                     /locus_tag="PKV56_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOX67460.1"
                     /translation="MFTRNTLAALAASVALITMTGCAVTRDQSTVGAYIDDTAITTSV
                     KARFVDNKEVDASAISVETLNGTVMLSGFAKNGTEKIRAEEIARAVKGVKSVKNEIAL
                     RG"
     gene            22790..23695
                     /locus_tag="PKV56_06305"
     CDS             22790..23695
                     /locus_tag="PKV56_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOX67461.1"
                     /translation="MTGVLGAVTSGARTLWAALTRYLDADGMRQGAAVSFYAAFSMAP
                     LLVVVAAVMVWLLGDDGAQVALLDALTRLIGERETKTLSELLARHASTPAEGAQAIFG
                     SWLALGTTLIGATGVFVELRSAVQAMLGERDGAFTWWRLVQVRLLAVGVVLGCGFLLS
                     VAMVAQTASLLALKWLTTEWAWLAPMLIAVEGLWSWGVITVIFAMMIRWLPNTRLPWR
                     DTVIGGAVAATLFMVGRYGISLYVATTAMQSALGAAGSFAALLVWVYWSSQIFLLGAA
                     LAVELGRPDPAVLPHPPVLREAAIG"
     gene            complement(23785..25230)
                     /locus_tag="PKV56_06310"
     CDS             complement(23785..25230)
                     /locus_tag="PKV56_06310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX67462.1"
                     /translation="MTFASLGLTEALTKAVADAGYETATEVQMKAIPLALAGKDLMVS
                     SSTGSGKTASFILPALERIIAARADTTQRRDRATVYGPRILVLTPTRELAMQVAKAAS
                     TYGRHVQGLRVATVVGGVPYPAQIKALRGPLDILISTPGRLLDHLQTGKAVLDHVEML
                     VLDEADRMLDMGFIDDINTIADHVPAARQTVMFSATFAGHVGRLAQNLLRDPQRIDVS
                     SHTDTHENIEQRLHWADNHNHKNALLDHILTEREMEQALVFTSTQRDADWLADRLAEM
                     GHAVASLHGGMPQGRRNRVLQGLRSRQLKVLVATDVAARGIDVPSISHVINYGLPMKA
                     EDYVHRIGRTGRAGKNGLAITLGERMDAGMIRRIQQFTTQNIPVSAIVGLEPKSPEPR
                     MYAPRPGADTERRPGGFGNRSGAPSNNRFSKPSFAPRREEGGFAPRRDDSFAPRSAGP
                     FDRAPRRPVEGGAAPGKAPFKARPRTGGFTR"
     gene            25689..26153
                     /locus_tag="PKV56_06315"
     CDS             25689..26153
                     /locus_tag="PKV56_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HOX67463.1"
                     /translation="MAGIHITDIEAAINWWRERSPSPDGITACSEVRALAEVYALMVY
                     YRESDCDDGSMPAASHAAWLAWYATTPDAPCIAICSTSQGDDICKGCGRSFDEVQDWP
                     VMSPAEKRATWRRITMDGTALRFNRYAERAMEVQRPAADESAASPSRQPGPA"
     gene            26164..27609
                     /locus_tag="PKV56_06320"
     CDS             26164..27609
                     /locus_tag="PKV56_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX67464.1"
                     /translation="MTPLRSWWSSAARILPLAWPVFIGQLAVLAFATIDTLMVARHSA
                     IDLAALAVGNAAYVSVFIGLTGVVQAVAPITGQLFGARQLPQAGQQLHQALWLALGLS
                     FLGCSLLVFPAPFLALAQAPPDVAEKVRAHLGALAFALPSALLFTAFRGFNTAVSRPK
                     MVMALQLVGLTLKVPLTALLVFGAALPLPAGWPWSEWRIPSLGTPGCGIATAIAMAVQ
                     LLVAAAVLLRSPFYAPFGLHTRRLQRPDPRKLAALLRLGVPMGLSIGIEVTGFTFMAL
                     FIARLGATPVAGHQIAVNLISVMFMLPISLGNATSTLVAQRIGAGDPLDARRLGWHGL
                     QLAVLIAATLGASVYALRDAILHVYTNDAVIVAAALPLLAWVMLFHIADAAQIHVAYT
                     LRAYRIASAPLIIYGIAIWGVGLGGGYTLAFDVTGFAPPALRGAPGFWAAATAGLTLA
                     ALGLAGFLGWMQRETASHQRTPRIDAHDSAS"
     gene            27686..28138
                     /locus_tag="PKV56_06325"
     CDS             27686..28138
                     /locus_tag="PKV56_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX67465.1"
                     /translation="MYDSYLSVPSGFLSDLDRMRGDIDSLFGWGGTSRAIRSTGGSTY
                     PAINVGQSPEAVDVYVFAPGIDASRTEVTLDRGVLTISGERASDLPAEAPKTSVHGRE
                     RYAGKFRRAVALPDDVDPNQVSATYRDGVLRVNVRRREAAQPKRIAIE"
     gene            28176..28547
                     /locus_tag="PKV56_06330"
     CDS             28176..28547
                     /locus_tag="PKV56_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX67466.1"
                     /translation="MRDTKPNNASPAPTPFMVPQVDVVEDSSGITLLADLPGVKKDGL
                     DIKVDGQTLSLEGTVALDAPQPFDAVFAEVRASRYRRSFTLSNELDSAAIDAQLKDGV
                     LRLRIPKVAEAQPRRIEVRVG"
     gene            28540..>28870
                     /locus_tag="PKV56_06335"
     CDS             28540..>28870
                     /locus_tag="PKV56_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-alkenal reductase"
                     /protein_id="HOX67467.1"
                     /translation="MAEPARSTGAATLLLIGAVAAAMFWLGQHSAAEALAATPPAASV
                     VPRAVVARGELAADERNNIDVFKSASPSVVHITTLERAASLFSIDATQVPRGTGTGFM
                     WDDHGHIV"
BASE COUNT         4878 a         9808 c         9440 g         4744 t
ORIGIN      
        1 cgcgaacgtg gccatgtgct cgagggactc gcggtggcac tggccaacat cgacgagttc
       61 atcgagacca tcaaggcgtc cccgacacct ccggtcgcca aggccgcgct gatgagcaag
      121 agctgggatt cgtcgatggt gcgcgagatg ctgtcgcgcg ccgaaagcga agcgcccggt
      181 ggccgcgaag ccttccgccc cgaaggactg ccccgaagct acggcctgca ggcggatggc
      241 ctgtaccggc tgagcgacga ccaggccggc gaaatcctgc agatgcgtct gcaacgcctg
      301 accgggctgg aacaggacaa gatcatcggc gaatacaagg aggtgatgac caccatcgcc
      361 gacctgctca acatcctggc gaccccggag cgggtgacgt cgatcatttc cgacgaattg
      421 gcagccatca agcaggagtt cgggcagacc aagatcggcg cccgacgcag cctgatcgag
      481 cacaacgcgc aggatctggg caccgaagac ctgatcacgc cgaccgacat ggtcgtgacc
      541 ctgagccaca ccggctactt caagagccag ccgctcgccg agtacggtgc gcaacggcgc
      601 ggtggccgtg gcaaacaggc cacgcagacc aaggacgacg actggatcga gcagctgttc
      661 atcgcaaaca cgcacgacta catcctgtgc ttcacgaaca agggacgggt ctactggctc
      721 aaggtctggg aagtgccgca gggttcccgc aattcgcgcg gcaagccgat cgtcaacatg
      781 ttcccgctgc tgcccggcga gaaggtcaat gtcgtgctgc cgctgaccgg cgaggcacgc
      841 acgttcccgg ccgagcgctt tgtcttcatg gccaccgcca agggcacggt caagaagacc
      901 tcgctcgacg aattcagcaa cccgcgcaag gccggcatca tcgccgtcga tctggacgaa
      961 ggcgatttcc tgatcggtgc ggccatcacc gatggtcagc acgacgtgat gctgttcagc
     1021 gacggcggca aggcggtgcg attcgcggag caggacttgc gcccgatggg ccgcaacgcc
     1081 cgcggcaaca agggcatgaa cctggagccg ggtcagagcg tgattgccat gctcgtggct
     1141 gaggatgaaa cgcagagcgt gctcacagcc accgagcacg gctacggcaa gcgcaccagc
     1201 atcgtcgaat acacccgaca tgggcgcggc accaagggaa tgatcgcgat ccagcagagt
     1261 gaccgcaacg gcaaggtggt cgcggccacg ctggtgaggc ccgacgacga gatcatgctg
     1321 atcaccgaca agggtgtgct cgtgcgcacc cgcgttgccg agatccgcga actcggccga
     1381 gcgacccagg gcgtgacgct gatcgcgctc gacaccggct ccaccctgag cggcctgcag
     1441 cgcatcgtcg agaacgacgt cagcgcagaa ggcacccccg acaccactgc gggcgatgac
     1501 agcgggtcgc ccaccaccga gacaccttga tgaaacgact tttcgcactc accgccctgt
     1561 gcctcgcgct gcaggctcag gcccagtcga cccctgcgcc tgcggcggcg ccggtttcgc
     1621 aggccaagaa ggaactcgtg cagaagctgc tcacgctgca gcagccggtg ttcgagacca
     1681 tcgcgcgcaa cgtcgtggag cgccccgcca tgcagatgat gcaggcggcc gcccaggccc
     1741 ttcaaacgca ggttcccgca gagaagcggg aggccaccgg caagagcatc gaaaccgacg
     1801 tgcggcagtt cgtcgaggag tcggcgcccg tactgcggga gcgcgccgtc aaactggcac
     1861 cgtcgacttt cggtgcagtg ttcgaagaga aattcaccga agacgaactc aagcagctga
     1921 tcgcgtggac cgagtcgccg gtgaacaaga agttcaagca gacgctgcct gaagtggaga
     1981 cgggcttcgt caagaagctc atcgccgagg ccggccccct gctcgacccc aagctgcagg
     2041 cactgcagca gaaggtgcgg gccacgctgg tcgccaatgc agcgccggct ccagcggccg
     2101 ctgcttctgc ggcccccgcc acgaagcctg cgcctgctcc cgccgcgaag cctgcggcaa
     2161 aggccgccag caagtaatcg gttcatgcct cgcccgttca atttttcggc tggcccggcg
     2221 acccttcctg cggaggttct ggaacaggcg gcctccgaga tgctgagctg gcatggatgc
     2281 ggtgtcagcg tgatggagat gagccatcgc ggacgcgagt tcatgtcgat ctgcgaacag
     2341 gccgaaagcg atctgcgcga gttgctgtcg gttcctccgg agtttcggat cctgttcatg
     2401 cagggcggcg gcctgggcca gaacgcgatc gtgccgatga atctgatcgg tcgatcgagc
     2461 agccgcaccg ctgatttcgt gctgaccggg tcgtggtcga tcaaatccca ggccgaagcg
     2521 cggcgctatg gcgaggcaaa catcgcggcc agctcggcaa cggggcgccc gacgcagctg
     2581 cccgatccgt cgacctggca catcagcaag gaccccgcct acctgcatct ctgcagcaac
     2641 gaaaccatcg atggcatcga gttccaggag ctacccgacc tgcgcgccct gggctgcaca
     2701 gccccactgg tgatcgactg ctcgtcccac gtcgcctcgc gtccgatcga ctggagccga
     2761 gtgggactgg cctatgcggg agcgcagaag aacatcggcc ctgccgggct gaccctggtc
     2821 ttcgtgcgcg acgacctgct gggccaggcg ctgccgatct gcccttcggt gttcaattac
     2881 cgcaccgtcg cggatcaggg atcgatgttc aacacgccac cgacctacgc gatctacatc
     2941 gccggcctgg tgttccagtg gctcaagcgt cagacggaat ccggcctgac cggcatcgcg
     3001 gcgatcgagg tgcgcaacca ggccaaggcg aagctgctgt acgactgcat cgacgcctcg
     3061 aggatgtacc gcaacgaagt cgatccgtcc tgccgctccc ggatgaacgt tcccttcttt
     3121 ctgtccgacg tgtcgttgac cgagccgttt ctagagcagg cacgcgacgc cggcctgttg
     3181 cagctgaagg gccacaagtc ggtcggcggg ctgcgcgcga gcctctacaa cgcgatgccg
     3241 ctcgaaggcg tgcaggcgct cgtcaaccac atgcaggcgt tcgaggcaag acatggctga
     3301 ccagcccacc gcaatcaatc cagaactgct ggccctgcgc acgcagatcg atgcgctcga
     3361 tcgtgacctg ctcgcgctgc tgaaccagcg ggccgcactg gcactgcagg tcggcgaggt
     3421 caagaagaag gaaggatcgg tcgcgttccg acccgagcgc gaggcccagg tgatcgatgg
     3481 actgaaggcg ggcaatcgcg gaccactgtc atcggacagc gtcgcgccga tctggcgcga
     3541 gatcatgtcg gcctgtcgtg ccctggagac accgacccgg gtcgcctacc tcggaccggc
     3601 cggcacgttc agcgaatcgg ccgcgctcgg tttcttcggt tcatcgatcg tgaaagtacc
     3661 ctgctcgagc atcgacgagg tgttccgggc cacgaccgcc ggcgccgccg atttcggtgt
     3721 cgtgccggtc gagaattcga ctgaaggcgt ggttgcgcgt tcgctggacc tgttcctgac
     3781 gacgccgctt ttcatcatcg gcgagacaag catcttcgtg cggcacaacc tgctgcgtcg
     3841 agacaattcc ctcaccggca tcgaagcggt gtgcgcgcat ccgcaggcgc tggcgcaatg
     3901 ccacaactgg ctcggcaacc acctgccgaa tgccgagcgc cgacccgtgt ccagcaacgc
     3961 ggaaggcgca cgactcgcca gcctcgaccc ccgactggct gcagtggcaa gcgaccgcgc
     4021 cggcagcgaa ttcggcctgc acatcgtggc gccggccatt caggatgacg cccacaaccg
     4081 cacgcgattc gcggtggtga cccacccaca gcgccatccg cagccccagg cttcggggca
     4141 cgattgcacc agcctcgtgg tgtcggtggc gaaccggccg ggcgccgtct acgacatgct
     4201 gatgccgctg aagacccacg gtgtgtcgat gagccgcttc gaatcgcggc cggcccgttc
     4261 gggtcagtgg gagtactact tctacatcga cgtccagggt cacccgagcc agcccgaggt
     4321 ggcccaggcg ctgaaggaac tgcgcggggt gtgcgccttc ttcaagctgc tgggcaccta
     4381 cccgatcgac gtgcactgat gaaaccagac ctcaacgccg ccctgctcct gccactttcg
     4441 tcgacgggcc ggactgtcgg gcggccgcgt cggcgctgac catgttcaat caactcgggg
     4501 tgatcggctg cggcctgatg ggcggatcgt tcgcgcttgc attgcgccgt gcgggcctgg
     4561 tcaagcgcgt ggtcggctac agcaagtcgc cttccacgac cgagaaggcc agacgcatgg
     4621 gggtgatcga ccaggccgcc gaatcggcat tgctggccgt gtccggatcg gacatcgtgc
     4681 tcatggcggt gccggtggca gccagcgaag cgacgttccg cgccatccgc aacctgatcg
     4741 agcccggcgt gctgctgatg gacgtcggtt cgaccaagcg cgacgtcgtc gacgcggcgc
     4801 ggcgcgtgct gaaggaccgt ctttcgaact tcgtgccggc gcacccgatt gccggcaagg
     4861 aagtcgccgg catcgagcat gccgacgcca cgctgtacca gggccggcag gtgatcctga
     4921 cgccgctgcc gcagacgcag cccgaactca tccagaaagc gaccgatgcg tggtcggcgg
     4981 tgggttcgca ggtgctgcgc atgacccccg agaaccacga tgccgcgttt gcggcgatca
     5041 gccacctgcc gcatctgctg gcctttgcgt atttcaacgg cgtggcaggg cagccggccg
     5101 gacgcgactt cctgtcgctg gcgggtcccg ggttccgcga tttcacgcgc atcgctgcga
     5161 gcgatcccac ggtgtggcgc gacatcctgc tggccaaccg cgaagaattg ctgaagcagt
     5221 cgaggcagtt tcggcaggcg ctcgatgcca tggaatacgt gatgaccagc ggcaacgccg
     5281 aagcgctcga ggaattgata cgcgccgcgt cgaccgcgcg cgccggctgg tccatgaact
     5341 ccggcaagag cacccctcag cgctgatggc aggtgcgcat cgcggacgct gccgcggaaa
     5401 tgcatgctgc acactttgat cgacacccct tcgccccacg atgtacgcca ttcccttcat
     5461 cgacatcccc ccgcttctgc atgctggcgg cacggtgcgc ctgcccggct cgaagagcat
     5521 ctcgaaccgc gtgctgctgc tcgccgggct cagcgaaggc acgacgacga tccacgacct
     5581 gctcgactcc gacgacacgc aggtgatgct gacggcgctg cgcacactgg gctgccgcat
     5641 cgaagccgaa ggcgacagcc tgcacatcac cggtctgggc gggcgcctgc aggcgaagga
     5701 agcctcgctg tttctcggca acgccggcac cgcgatgcgg cccctgaccg ctgcgctggc
     5761 gatgctggcc gccacgcagg gcggaacctt cgaactcagc ggcgtgccgc ggatgcacga
     5821 gcgaccgatc gccgatctga tcgatgcgct gcgcccgctg ggctgccgga tcgactatct
     5881 ggggcaggcg ggctacccgc cgctgagatt gctgccaggt agcggcgcgc tcgacctggc
     5941 ggctcccatc cgggtgcgcg gcgacgtgtc cagccagttc ctgacggcgt tgctgctcgc
     6001 cttgccgctg ctgacgcaag accagcaagc caccgtcgag atcgacggtg aactgatctc
     6061 gaagccttac atcgagatca cgctgaacct gctggcgcgc ttcggcgtcg aggtgcagcg
     6121 cgacggctgg cagcggttca tcatccccgc cggcagccac taccggtcgc cgggcgccat
     6181 ccacgtcgag ggcgatgcgt cttccgcttc ctatttcatc gcgctggggg ccatcgcctc
     6241 gatcgacgcc ccgctgcgca tcgagggtgt cggcaccgac tccatccagg gagacatccg
     6301 gttcgtcgag gctgcccggg ccatgggggc ccaggtcacc ggcggcgccg gctggatcga
     6361 agtgcgccgc ggacgctggc cactggccgc gatcgacctg gactgcaacc acatacccga
     6421 cgcggcgatg acgctggcgg tgatggcgct gtacgccgac ggacccaccc gcctgaccaa
     6481 catcgcgagc tggcgcgtca aggagaccga ccgcatcgcg gcgatggccg ccgagttgcg
     6541 caagctcggt gccgtcgtgg tcgaaggggc tgatttcatc gagatcgccc cgccgctgca
     6601 ctggtcaccg gcgtcgatcc atacctacga tgaccaccgg gtggcgatgt gcttttcgct
     6661 ggcggcctgc aacccgctgg cagttccacc gccgtccgat ccggtcgccg tgcgcatcct
     6721 cgacccgaaa tgcgtcggca agacatttcc cgactacttc gaggccctct tcagcgtggt
     6781 cggcaccaag ccagagtgga tcccggtgat caccgtcgac gggccaaccg cctccggcaa
     6841 gggcacggtg gcgagcgccc tgtccgaacg actgggctat cacgtgctcg attcaggcgt
     6901 cctgtacagg gccactgcgc tggcagctct ccgaggcggt gttgactccg atgatgaagc
     6961 cgggctcgct gcgctggccg aggcgctcga catcggcttc gaggctggct cgattcacct
     7021 ggatggcgcc gatgtcaccg cacagctgcg ggacgaagaa gtgggtgcgc tggcctcgcg
     7081 gatttcggcc ttgcccgcgg ttcgccaggc gctgctgcgg ctgcagttct ccttccggca
     7141 actgcctggg ctcgtggccg acggacgcga catgggcacg gttgtctttc ccgacgccga
     7201 cctcaagatc tacctgacgg ccagcgccga agcacgcgcc cgcaggcgac accagcagtt
     7261 ggaagcttca ggcgtttcgt ctacactcga gggcttgctt gcaggtttgc gggagcgtga
     7321 cgcgcgggat cagaaccgag ccatcgcccc gctgaaacca gcggcagacg cggtgcttct
     7381 ggacaactcg gcgctgacca tcgaatcaac ggtggaagcg gtgaagtcgt gctgggagca
     7441 acgaagaccg ttcgcctgac cgcggacaac ccggcccagc gccttccctc cgtgccgttc
     7501 gaggcacacc gaggcgccgg attcgaccac tccaacccgc agcacgtgct gcaccggaaa
     7561 caccccatgt cacaacccca aactgccatc accgaaggcg gcgaatcgtt cgccgacatg
     7621 ttcaacgaat cgctgacgcg ctccgaaatg cgcatgggcg aggtcatcac tgccgaagtg
     7681 gtgcgcgtcg aacacagctt cgtggtggtg aacgcaggcc tcaagtccga agcctacgtg
     7741 cccatcgagg aattcaagaa cgaccacggc gagctcgaag tccaggtcgg tgatttcgtc
     7801 tccgtcgcga tcgacgcggt cgagaacggc tacggcgaca ccatcctgtc gcgcgacaag
     7861 gccaagcgcc tcgcgtcgtg gctgtccttg gaaacgtcac tggagtcggg cgagttcgtc
     7921 accggcacgg tgaacggcaa ggtcaagggt ggcctgacgg tgctggtcaa cggcatccgt
     7981 gccttcctgc caggttcgct gctcgacacg cgtccggtca aggacatgag cccgtacgag
     8041 ggcaagacca tggaattcaa ggtcatcaag ctcgaccgca agcgcaacaa cgtcgtgttg
     8101 tcgcgccgcg ccgtggtcga agcctcgatg ggcgaggaac gcgccaagct gctgggcacg
     8161 ctggccgaag gcgccatcgt gaccggcgtg gtcaagaaca tcaccgagta cggtgcgttc
     8221 gtcgacctcg gcggcatcga tggcctgctg cacatcaccg acatggcctg gcgtcgcgtg
     8281 cgtcacccga gcgaagtggt gcaggttggt caggaactgc aggccaagat cctgaagttc
     8341 gacgccgaga agaaccgcgt ctcgctgggc atcaagcagc tgggcgacga cccgtggcac
     8401 ggcgtgtcgc gccgctaccc gcagggcacg cgcctgttcg gcaaggtcac gaacatcgcc
     8461 gactacggcg cgttcgtcga gatcgagccg ggcatcgaag gcctggtgca cgtctccgag
     8521 atggactgga ccaacaagaa cgtcgccccc gccaagatcg tgtcgctggg cgacgaggtc
     8581 gaggtgatgg tgctcgagat cgacgaggac aagcgccgca tcagcctggg catgaagcag
     8641 tgcaaggcca acccctggga agagttcagc gccaacgtca agcgcggcga caaggtcaag
     8701 ggcccggtca agtcgatcac cgacttcggc gtgttcgtcg gcctggctgc aggcatcgac
     8761 ggtctggttc acctgtccga cctgtcctgg cacgagccgg gcgaagctgc agtgcgcaac
     8821 ttcaagaagg gtcaggaagt cgaagcgatc gttctggccg tcgatgtcga acgcgagcgt
     8881 atttcgcttg gcatcaagca gctcgacagc gatccgttca ccacctacac ctcggtcaac
     8941 gaccgcggca gcatcgtgac cggcaaggtc aagacggtgg atgctcgtgg cgctgaagtg
     9001 cagctcggcg acgacgtgac gggttacctg cgcgcttctg aaatcagccg cgatcgcgtc
     9061 gaggacgccc gcaacatgct caaggaaggt gacgaagtca atgccctgat catcaacgtc
     9121 gaccgcaaga cgcgctcgat ccagttgtcg atcaaggcca aggacaacgc cgacaaccag
     9181 gaagcgatgc agcgcctgtc gcagaccagc gagcgtgaga acgccggcac gacgagcctg
     9241 ggcgccctgc tgcgcgccaa gctcgacaac cagggcggca acccctgatc gttggagcgg
     9301 ccgaggtcat ggacgcaatg aaggatggca tgacgcgttc ggatctggtg agtcggctgg
     9361 ctgagcgttt cagccagctg acgcaccggg acaccgagtc ggcagtcaag atcgttctgg
     9421 atgcgatgtc cgatgcgctc gcgcgcgggc accgcatcga aatccgtggt ttcggcagct
     9481 tctcgatcaa ccgccgcccg ccccgcgtgg gtcgcaaccc gcgctccggc gaacaggtgg
     9541 tggtgccaga aaagctggtt ccgcatttca agcccggcaa ggccctgcgc gaggcggtgg
     9601 acatgaacct cccggccgac gggcctccac cgaccccggc aggcacgacc cgaacctgac
     9661 acggcggcga aatccgatag gctggcgccc ggaagtcgcg atgtcgcggc ttcgcccgat
     9721 gggccgatgc acgcagtggg ccctgttgtt gcggagacgt catgcgcgtg atcacctggg
     9781 ttttcagagt cttcgtcttc ttcgcgttgt tcgcgtttgc cctgaacaac aagcaggagg
     9841 ccggggtcaa ctggttcttc ggctacgaat ggcgagcacc gatggtcttc atcgtgctgg
     9901 ctgcattcgg cctgggctgc accttcggcg tggtcgcgat ggttccgagc tggtggaagc
     9961 accgccgcct cgcgaaacga ttacccgcat caccctccga ccgcgacctg cacaacccgc
    10021 ttgcggccgc acctccgata cccgagcatc cgccccgcga tggactttga cattcagtgg
    10081 gtgctgctcg gcctgcccgt cgcgttcgcg ctgggctggc tggcctcgcg cttcgacttc
    10141 cgccagctca agcgcgagca gcgcgatgca cccaaggcct acttcaaggg cctgaacctg
    10201 ctcctcaatg agcagcacga caaggccatc gacgccttca tcgaggccgt tcagcaggac
    10261 cccgacacca gcgacctgca ctttgcgctg ggcaacctgt ttcgccggcg cggcgaatac
    10321 gaacgcgcgg tgcgcgtgca ccagcacctg ctgaaccgcg cagacctgcc gccggcagaa
    10381 cgcgatcggg cgcagcacgg cctcgcgcag gacttcatgc aggccggcct gttcgaccgc
    10441 gccgagaccg cctggcgggc gctcgaaggt accgcctacg acaccgatgc gcgccttgcg
    10501 ctgctgacgc tgcacgagcg ctcgcgcgac tggcgcgccg ccgtcgacga tgcccagaag
    10561 ctcgaacgcg gaagcaccgg ctcgtatgca tcgcgcatcg cgcactactg gtgtgaactg
    10621 gcactcgagg cagacgcgaa acagcagccg accgacgccg acgacgcgct gcgccgcgcg
    10681 cacgaggccg cgccccaggc cgctcggccg atggtgatgg ctggcaggcg gctggcgcgg
    10741 cagggtcgcc atgccgaggc cctggaggtc tggggcgagt tgctcgccag ccatccatca
    10801 agcttcaatc ttgtcgccgg cgactacgcc gccagcgcca tcgcctgctc ggcgcaggac
    10861 accgcccggc ttcggctgct ggccctgcac cagaagaagc cgagcatcga cctgctcaac
    10921 gcgatcggag cactcgacac cgacccggcg tcgcgacgcg accgcatccg cacccagctg
    10981 tcgctgctgc ccacgctgtc ggcagcccag gcggtgctgc atgagcacga ggcatcggcg
    11041 gtgccgttgc tgcccgccga cctcgaagca gtcaacgagg tgatcggtcg tgccgccaag
    11101 gccggccagc gctaccgctg cgcggcctgc ggtttcgagg cgcagcacca tcactggcag
    11161 tgtcccggtt gcctgggctg ggacacgtac ccgccgcagc gcctcgaaga gctctgatcg
    11221 cacgtgccca tggcccgctc agcgtcgaac gacctctacc tgcgcctgat caagcgcatt
    11281 cgcccgcact ggcgaacctt cgcgctcggc atcctcgcga tggttggcta cgccgccacc
    11341 gagaccgcgc tgcccgcctt gctgaaggaa ctgctcgacg gcagcttcgt caatcgcgac
    11401 ccgtcgtcga tccacgcgat gccgatcctc atcgtgctgt tgttcctggc tcgcggcctg
    11461 accgatttcg tgcatgtcgc gacgctgaac gcggtcgcca gcaaggtggt gctcgaactg
    11521 cgcacggaca tgttcgacaa gctgctgcac ctgccgacca gccacttcga ccgcgagccc
    11581 agcgcggcgc tgatgtccaa gctgacctac aacgcccagc aggtcagccc gatcatcacc
    11641 gtgtccctga tcaccatcgt caaggacagc ctgatcgtcg tcggactcct cggctacatg
    11701 ctctacctga actggcagct ctcgctggcc ttcttcaccg tgctgccggt gattgcctac
    11761 gtgatccgct ccgtcagtcg gcggctgcgc agcctcagcc acggccagca gtcgtctatg
    11821 ggcacgatgt ctcacgtgat cgacgaggtc atcggcgggc atcgcgagat caagatcttc
    11881 gctggcgaag cctacgagtc gaagcgcttc catcaggttg ccagcgcgtt gcgccgctac
    11941 acgatgaagg cggtgacgac gtcggccgcc aatggtccga tcgtccaggc catcgcggtg
    12001 ctcgcgctcg ccgtcatcat ctactacgca tcgatccagt cgagccagaa ccagctgacg
    12061 gtcggcggct tcgtttcgtt cttcagcgcg atggccctgc tgctcgctcc gctcaagcgc
    12121 ctgtccaacg tcaacgaggt gctgcagcgg ggcctggcgg cggcggccag cgtcttcgaa
    12181 gtcatcgaca cgccgaacga gcccgacgct ggaacccggg cgctcggccg ctcgaccggt
    12241 cagctgcaat tcgagggcgc ctgcttccgc tatccacagg ccgaccgtga tgcgctcgtc
    12301 gacatcacgc tggacatccg gcccggcgaa tcggtggcgc tggtcggatc gtcgggcagc
    12361 ggcaagtcga cgctgatgtc ggccatcccg cgctttcatg cgctggatcg cggtgcgatc
    12421 cgcctcgacg gcatcgacat ccgcgaactg cgtctggccg acctgcgcgc caacatcgcg
    12481 ctggtgacgc agcatgtggt gcttttcaac gacacggtcg ccgccaacat cgcctacgga
    12541 ctgaagaaca ccgcgtccga agcggacatc gtcgccgctg ccgaagcggc gcacgcgatg
    12601 gacttcatcc gcgagatgcc gcagggactg gcgacgccga tcggcgaaaa cggcgcccgg
    12661 ctgtcgggcg gacagcgcca gcgcatctcg atcgcccgcg cgctgctgaa ggacgcaccg
    12721 atcctgctgc tcgacgaggc cacctcggcg ctcgacaccg aatcggaacg cctggtgcag
    12781 atcgcaatcg acaacctgaa gcgcggccgc accacgttga ccatcgcgca ccggctgtcc
    12841 accatcgcca acgccgaccg ggtggtcgtg atggaccagg ggcgcatcgc cgaggtcggc
    12901 acgcacgccg aactgctggc gctggacggc atctattgcc ggctgcatcg catgcagttc
    12961 ggcaccgccg atgccgcacc gacggccacg gtggcaccgg tcgccgatgc attgatctga
    13021 tggctgcggc gcacctccat ctccgggccg catgaccgag acatccgacg aacgaccgcg
    13081 catcctgatc gtccggctca gcgccatcgg cgacgtggtg atggcgagcg gcctgatacc
    13141 cgcgctgcgt gaccgctggc cgcatgcgca tctcgcctgg ctggtcgagc cggccgccgc
    13201 gccgctgctc gcgcacaacc cccgactcga cgaactgatc gtctggccac gcggcgagtg
    13261 gcgtgaactc tggcgcacgc atcgctacgc cgcactgctt cagcgcttcc tggcgctgcg
    13321 cagccgcttg cgtgccggcc gctacgacat cgccctcgac acgcagggcc tgctcaagag
    13381 cggcgtctgg gcctggctca gccatgcgcc gaggcgcatc agcctgatcg gacgcgaagg
    13441 cagccagcac ctggcgaccg agcgggtgca ccggccgcag gagaccggtg tgccgcgacg
    13501 cctgggatcc gaataccgcg tgctcgccag tcacctcggt gcggctgacg aggcctttca
    13561 gcccgacctg gccatcggcg ctgcggcgcg cgaagcggcg cgcgcggcac ttcgttcggg
    13621 atcggcggtc atcaccgccg gcggacggta tgccgtgctg tgcccgttca ccacccgacc
    13681 acagaagcac tggttcgagg accgctgggt ggcgctcgcc ggacgatgcc acgacgacag
    13741 gctggtgccg gtgctgctgg gtggcccggg tgaccgggag gccgcagcgc gcatcgcggc
    13801 cggggcgccc ggactggtca atctgaccgg tgcgctcaag ctcgacgaga gcgtcgcggt
    13861 catagccgag gcctgcgcgc tggtcggtgt cgacaccggt ctgacacaca tgggcagcgc
    13921 cctccacgtt ccgacggtcg ccctgttcgg ctcgacctgc ccctacctcg acgcgggtac
    13981 gccgcgcagc agcgtgctgt acgagaagct cgcctgttct ccgtgccgcc gcaatccgac
    14041 ctgcaacggg cggttcgact gcatgcgagc gctcgacgcc gaccgggttc acgccgagat
    14101 gcggcgggtg atggcgctgc cggcacccgg cacgcaggtg caccgatgag caccacggcg
    14161 gtctatttcg tcgcatcgcc gttgcaactg ctggccgcgc agcagatcgc gcgcacccat
    14221 gaggccggat cgagacaggt gctggtgtgg taccagccgg gcgtcggcac gcacgtgcgc
    14281 ccagcggact gggacgccag cagctacatg ccgtggccgc gatggaagcc gctgccaggc
    14341 tggttcggac gccaccgccg gctgcttgcc aacatcgatc tggtcgccgc actgatcggc
    14401 cgatgcgaca ccttgcacct gcacagcgcg gtgttcgaca ccgaggcgat caactacttc
    14461 ctgcgcgccc tgccggcccg ctgcggcgcg cgcacggttc gcggacgcat cctgcccgac
    14521 ggcctgatca gcatccgccg gtatccactg tcgccgatcc gccgctggtc gccctggttc
    14581 cgcaagctgc gtcgactggt atcgcccgag ttgaactact ggctgttcac aggcgaccga
    14641 atcggctcag atgcgtcgtt ctgcgaccgc atctacgtgc tgtcggactg gccgcacgag
    14701 tacgacccgg ccaaggtcgt cgtcctctcg ccgctctcgc agcgtgcgcc gaccgatgcg
    14761 ggttcggtgc tgccctcgcg cgcgctggtg atcgggcagc cgctggtcgg agccggcctg
    14821 atgggcacgg cggatcgcga tgcagtcgcg aggcagatcg agcaatggct gacctcccgg
    14881 gaaatcgcgc aagtcgacta caagggccat ccgaaggacc cgcagcgcga gttgtgccag
    14941 cctggctacc gggtgctcga tctcgacgag ccactggaga tctggctggc ccacacaccc
    15001 taccaggctg tcgtcggcgt gcgctcgacc gcgctgctgt tcgcccgcca gctgtgcgcc
    15061 gcgcagaccg aggtggtcgc cttcggatgg gagcacaccc gcttcaagtc acagcaggaa
    15121 cgcgacgaca tgcgcgcggt gttcgatcgc gtgggagtgc gtcaggcatg agccccccga
    15181 cctggcggga ccgcatcccg gccatcaact cgtcgctgct ggcggcgctg ttctttctca
    15241 tcccgacgca gatcgcgcca gcctacgtgc tgtcggcgct gatgctcgcg ctgtggctcg
    15301 tcgaggggcg actgcaagcc aagtggcagg tgctgcgcgt gaatccggtg ttctggatat
    15361 tccaggccta cttctgctgg tacgtcgtct ctctggcgtg gaccgccgac atgaccgcag
    15421 gagccagcat ggtcacgcgg cacctcttct tcccgctggc ggcgctgtac ttcaccgtgg
    15481 cgcggcgcga gcacacccag cgctacctcg tggcattcgc cgcgggcgtg ctgttgtgcg
    15541 aactgctcgc cggctacaac tggctgcagc tgaacctcta tccccactgg ccggccagcc
    15601 tgcgcgcggc caaggacgcg atggaaaccg cgcccttcgt cgaccgcatc ctgttcggcc
    15661 cgaccctcgc cttcgccggc tacatcaccg cctggcaggc gctggtctgc cgccgggccg
    15721 gacagcgcgg tatcgcggtg gcctgggcag cggcctgcct ggcgacgatc ggggtgctga
    15781 gcttttccgg cagccgcact ggcatgctca gcttctcgct gatgatggcg gtgctgaccc
    15841 tgcaggccct gaaacgccat cgcgcgtggg ccgccctctg cgcggccatc gtgctggtgg
    15901 gaactgcggc ggggctctac gcgatgagcg acggcacgac ccgtcagcgc gtgcttgacg
    15961 gattcgccga ggcccgcaac ctcgacgcag cggtcaacct gtcggtgccg ctgcgctaca
    16021 cgatggctgt caacacgctc cacatcatcg ccgaacagcc ctggctgggg gtaggagcag
    16081 gcgacttcgt cagcgcctac aaggacatga acacgcggcg ctctccggcc tggaatgcac
    16141 cgcgaaaccc gcacaaccag ttgctgttca ccgtggcgac gaccggcgtg ttcggcggtc
    16201 tgctgctgct cgcggtgtgg ttcgcgcctc cgtggctcaa ccggaacctg aacgatggcc
    16261 tgggcgcact gcgggtcggc ctgccggtct tcttcttcac ggtatgcctg gccgagtcct
    16321 acctgtggcg caccaacacc gccttgctgt tcgccctgtt ctcggcgctg ctctacggcc
    16381 ccgtgccaac gctgcaccca cccgccgact tgcgtccgac ccccacttga accgccgccg
    16441 cgcccgatga cccgaagccg agcgcaggca tcgcgccggt gcgtcttcca aagccgatac
    16501 gatcgccgtc aggcaggcgt gccttcgcgc gccccggttc caacccgaaa ggacagatca
    16561 tgcaaaccac ttcttccgga ctgcagtttg aagacaccat cgaaggcacc ggcgaggagg
    16621 ccgtgtcggg ccagcatgtc agcgtgcact acaccggctg gctctacaac ggaggcgtca
    16681 agggcgccaa gttcgacagc agcaaggacc gcggcgacgc gttcaagttc tatctgggcg
    16741 ccggtcaggt gatcaagggc tgggatcagg gcgtgcccgg catgcgcgtg ggcggtaccc
    16801 gcgtgctggt gattccgccc gagctgggtt acggagagcg cggcgccggt ggcgtgatcc
    16861 cgccgaacgc gacgctgatg ttcgaggtcg agttgctcga catctgaccg ccggttcggc
    16921 gacagcagca caccaggccg accccatgtc gatccagtac gacatcacgc acacgaccgt
    16981 ctaccgctac agggagccgg tgagtttcgg cgagcaccgc gtgatgttca gaccgcgcga
    17041 cagccacgac ctgcgagtgc tggccaccga cctgcaggtc agccccgagg ccatcgtgcg
    17101 gatgatccag gatccgcact ccaactcggt cgcattggtg cagccgctga cctcggcgct
    17161 cgaactgcgc atcgtgtgct ccttctcgat cgagcacgct cacaccaaca acctggagct
    17221 gccggtgtcg gggctcgccg agttcttccc gttcgcctat tcgaccgacg agcgattcga
    17281 cctcgagatg tacctgcgcc cccatcacga ggacccgcag ggcaaactga tggactgggc
    17341 tcgccagttc gtccgcaccg acggtcccaa ccgcacccgc gacatgctgg tcgcgatgaa
    17401 cgagcacata cgcaacggtc tggtctacca gggccgcgag gaagaaggca cgcaatctcc
    17461 gctggacacg ctggcactcg gcagtggaac ctgccgcgac ttcgcgctgt tgatgatgga
    17521 agcggcacgc cgattgggcg tggcaacccg tttcgtatcg ggctacatct acgacccggc
    17581 cctcgacacc gccacgacgt tcgacgacgc cggtgaaccc ttgtccacgc agggcgccgg
    17641 cagcacccac gcgtggctgc aggcctatct cccgggcgct ggctgggtgc ccttcgaccc
    17701 gaccaacaac ctgcttggcg gcagccagtt gatccgcgtg ggtgtcgcgc gtgatccgag
    17761 cctcgcagca ccggtatcgg gcagctggta cggggcagcg gacgcctatc tgggcatgga
    17821 ggcgtcggtg caggtgcgcc gggtggtgtc tccggcatgg cagggtacgg ctgccaccga
    17881 tgccgagtcg ctcgcggtgt aggacacatc cgacagacgc tgcgtggttt ggacggcaag
    17941 agatgccggt gccgctgacg agaatcagcg catattcaag cgatgatcca gcaccgctca
    18001 ccgtgcccgt ccggcaccga gctccaaccc ggcatcttct tcacgcagca cgcatgggac
    18061 acctgaaaac ctacatcgtc gaggacagcc ccgtgatacg cgagaacctg atcgctgcac
    18121 tggaggaact cgtcagcgtc gatatcgtgg gcactgcggc agacgagaac acggcagtgc
    18181 aatggatgtc gagcaattcc cagtcgtgcg acctgatgat cgtcgacgtc tttctggcgc
    18241 agggttccgg gctgggggtg ctgaaggctg cgcgactgca cgacaagccc accaagctcg
    18301 tggtgctgag caactacgcc accccggaca tgcggcgcaa gtgcatcgaa ctcggcgcag
    18361 accgggtctt cgacaagtcc aacgagatcg acgccttgat ggcctattgc ctgcgcctgt
    18421 cgaccggtga ttcgggaaac agcgccttca gctccctcag ctgaacgccg ccgcgcgcag
    18481 acgcccgccg cacaacatca ctggatcaac ccggctttca gcgcatagta ggtgaggtcg
    18541 ctgttcgact cgagcttcat cttttccatc acgcgggtgc ggtaggtgct caccgtcttc
    18601 acgctcaatg acatgctgat ggccatcgtg ccgatcgcct ccccctttgc cagccgcagg
    18661 aagacctgca actcccgttc ggagagcaac tcatgaggca gcttctcccc cccgccggcc
    18721 agtccgtcgg ccagcagttc ggccacaccc gaagtgatgt acttgcggcc gcgaaacacc
    18781 gttcggatgg ccttcgcgat ctcctcggga tcacagtcct tgttgaggta gccggcggcc
    18841 ccctgcttga gcatcgtggt ggcgtagtgc tgttcgggaa acccgctgag gatcagcacc
    18901 ggcaggtccg gcgcgcgcgc cttgatcgcc gccagcacct cgacaccgct ctgatcgggc
    18961 atcgacagat caaccaccat cacgtcgacg ccgccggcgc gcacgatgtc gagcgcatct
    19021 cgcccgttcg cggcttccgc cacgacggtg aggccgatct cctcggacag gaactgacgc
    19081 agtccggtgc gcacgatggc gtggtcgtcg acgatggcaa tgcggatcat gttgacgagg
    19141 cccgggccgg aagtggatgc aacgagttta ggggcacccc gtgtcgccgc cgctgatcgg
    19201 ccgattctgg cgcagcgcct acgcctttcc ggtggcctgc gtggcggcga tcgtcctgct
    19261 ggcattcagc gaggtttcgt tcaggagcgc ttcgaattcg atggagcgcc tcgaggcgat
    19321 ggccagggcc cgcaccgaca tccagtcgct gtggcgccag ctggtcgacg cggagaccgg
    19381 ccagcgcggc tacctgctga cccagcgcaa cgagtacctc aagccttacc gggagtcact
    19441 cgagcgcatc gacgcgtcac tccattcact gacgacgtac taccgcggtc acccgcaggg
    19501 agcgcccctg atggagcgcc tgtccggcct gagcaaggac aagatctcgg agctggcaac
    19561 gaccctgcat ctgcaggacg agggcggagg cgcctggcgc gatttgctga tgaccaacat
    19621 cggccaggag aagatggaat cgatacgcag caccgcacag caacttctcg cgctggagac
    19681 ccagcgcgtt gccgaaggtc gcgccgacgt gcatcgcacg ctgatgatca accgggtcgg
    19741 cgtcggcctc acgacgctcc tcagcctgct ggcgctggcg ctgtacttcc ggcagtcgag
    19801 cgcgctggtg cgtgtgcagc gggagcagaa gcaagcggtt caggtggagc gcgaccgcct
    19861 cgatgcggag gtgaaaagcc gcaccgccca gctgaccgaa ctggcgcagc acctgcagac
    19921 cgcacgtgaa gacgaacgca gccgcctggc gcgcgaattg catgacgaac tgggcgcgct
    19981 gctgaccgcg gccaagctcg atgccgcgcg cctgaaggcg cggctgggga cgatgtcgcc
    20041 cgaagtcgcc gagcgactgc tgcacctgaa caagctgctc aacgagggca tcgcgctgaa
    20101 gcggcagatc atcgagaacc tgcggccgtc gtcgctcagc aacctgggcc tggtggcggc
    20161 gctcgagatc gtcaccagcg aattcgccac ggcttcggga ctgcaggtgc acgcggagct
    20221 ggagccggtc gaactcagca gttcgggtga gctgaccgcc tatcgcctgg tccaggaagc
    20281 actgaccaac atcgcgaagt acgcgaaggc cagcgaggtc tgggtcgtcc tgtcgcagca
    20341 gggcgcgtca gccgcgatct cggtgcgcga caacggatgc ggcttcgaca cgctgacacc
    20401 ccgtgaggca gcgcacggcc tgctcggcat gcgctaccgc gtcgaagccg agggcggtca
    20461 catgaccctg tcgagccaga agggtcacgg cacgctgatc gaggcgcgcc tgccgctccg
    20521 accttcctga gcaacggcgc cgcggcggct gaccatcggc tgggcgacgt cgcggtcccg
    20581 ccgtcggtcc gggcaccacc ccaacctgcc gcagcggctg ccaacgcgat ggtccgtcgt
    20641 gtcggtcccg tcctacacgc acgggccgaa ttgcctgacg ctcgagaggc gaggcggact
    20701 atcgattgcc gccgcgcgcg accgaacact ggagccctgc gatgcccggg ctcgttccga
    20761 ccatgcatcg ctccacttcc agggagatat ccatgttgca ttacgcggtc gtgttcttcg
    20821 tcatcgcgct ggtggctgcg ctgttcggct tcggcggcat cgccgcgagc gccgtcggtg
    20881 tcgcgaaggt gctgttcgtg atcttcctgg tgctggccat catcagtttc ctcttcggcc
    20941 agatgaggaa gtagagcgct tcaggcgccg ccgcttcgaa tccagacccc aaccctcacc
    21001 acccttcagg agtcacccat gagagtcacc atgcccagcc agttcgttgc gggcgccgcc
    21061 agcggagccg ccgatgccgt cgaccagatc gatcgcgccg aagagtccca tcgcctcctg
    21121 gcagaagaag cacatgacgc ggtcgatgcg gccgttccgg tgatcgatcg cctggtcaag
    21181 aacgccgaaa gcctcgcccg acgtggaatc gacggcgccc gcgacggctc gcagcaactg
    21241 cgcgaccgtg ccgtgcgcgc gaccgacagc accgtcaact acatcaagga cgagccgctc
    21301 aaggcgatgc tgatcgcggc tgcgaccggt gccgcgctga tggcgctgat ggccctgctg
    21361 aatcggtcac gccagagcga ctgacctggc gtctcgcaaa agcaccccca cccgcccccg
    21421 gaacacgcat cgtgattcac ccgcttttca aattgatggc cgcgcacccc acggtgctga
    21481 ccgatcacgt cgaggcctac gcatcgctcg cggtgcagga actcggcacc gctgcaacgc
    21541 aatggcgctc gcgggcgttg aagacgggca tcgcgatgtt gtgcgctgca tccgcagccg
    21601 ttctggccgg ggtgtcgctg atgctgtggg caacgacagc gcccaccgat ctgcaggcgc
    21661 cctgggcgct ctggatcgcg cccgggctgc ccgcgctcgc cgcgatggcc tgctggtggt
    21721 ccgctcagga aaagccggcc gaaggcccct tcgcgatcct acgcgggcaa tttgccgaag
    21781 acatggccct gctgcgtgag gtgaacgcgt gagcgctgcg gccacaccaa tcgccgatcg
    21841 cgccatggca gacgctggca ccgcaccggt cgatgcggtc gcaaggctcg gacagtccag
    21901 gttgcgactg cgtcaacacc tgatcgcgtt gaacggcaat caagccggtc ccgctggctc
    21961 gcaggcactg accggctggc ttggctcgct cagatcgatc ccgttgctcg gcaccgcgct
    22021 ggacacggtg tcgggctggt ggtctcggca cccattgcgc gtcgtcgcca ccgtgctcgc
    22081 ggcgtcgacc gcgtcggcgg tccgcccggt cgcccaacgg catccggcct ggagcgtgtt
    22141 cggcgcgctg gccgtgggcg ggctggtgat gtgggcgaaa ccgtggcgca tcgcgctgct
    22201 caggcgcgtg ctctacgccg gcttgctgcc gcagctggtg tcgaaagcga tgtcgcagca
    22261 gcccaccacc ggctggctga gcttggtgga atcggtgctg cggcgcagcc ccaccccagc
    22321 gccctccgcc ggcccggtaa cagcaatggt catccaccca aatcttcctg ccggcagcga
    22381 tttcacaagg acatcgccgt tgcgctagcc ggcctctgtg cccgcctccg cttttttcat
    22441 ccaccccaag gagctacatc atgttcacac gcaacaccct cgcggccctg gccgcctccg
    22501 tcgcactcat cacgatgacc ggctgcgccg tgacgcgcga tcaatcgacc gtcggtgcat
    22561 acatcgatga caccgcgatc accacgtcgg tgaaggcccg cttcgtcgac aacaaggaag
    22621 tcgatgcctc ggcgatcagc gtcgagaccc tgaacggcac cgtcatgctg tcgggcttcg
    22681 cgaagaacgg caccgagaag atccgggctg aagaaattgc acgtgccgtg aagggcgtga
    22741 aatcggtcaa gaacgaaatc gctctgcgcg gctgatccag ccgagttcga tgaccggagt
    22801 gctgggcgcc gtcacgagcg gcgcccgcac tctttgggcc gcgctgactc gatatctcga
    22861 cgccgatggc atgcgccagg gcgccgcggt gtcgttctac gcggcttttt cgatggcgcc
    22921 gctgctggtc gtcgtggccg ccgtcatggt ctggctgctc ggcgacgacg gcgctcaagt
    22981 cgcgttgctc gacgcgctga cccggctgat cggcgagcgc gaaacgaaga ccttgagcga
    23041 gttgctggcg cgtcacgcca gcactccggc tgagggcgca caggcgatct tcgggtcgtg
    23101 gctggcgctg ggcaccacgc tgatcggcgc caccggtgtc ttcgtcgaac tgcgttcggc
    23161 cgtgcaggcg atgttgggcg agcgcgatgg cgctttcacc tggtggcgac tggtccaggt
    23221 ccgactgctg gctgtcggcg tggtgctggg ctgcggcttc ctgctgtcgg tggcgatggt
    23281 ggcgcagacg gcgagcctgc tggcactgaa gtggctgacc acggaatggg cgtggctggc
    23341 gccgatgctg atcgcggtcg aggggctgtg gtcctggggt gtcatcaccg tgatcttcgc
    23401 gatgatgatc cgctggctgc ccaacacccg gctgccgtgg cgcgacaccg tgatcggtgg
    23461 cgccgtggct gccacgctgt tcatggtcgg ccgctacggc atcagcctgt acgtggcgac
    23521 cacggcgatg cagtcggcgc tgggcgcggc gggctcgttc gcggcgttgc tggtgtgggt
    23581 gtactggtcg agccagatct ttctgctggg ggccgcactg gcggtcgaac tgggccgacc
    23641 ggatccggcg gtgctgcctc acccacctgt cctccgggaa gccgccatcg gatgaataac
    23701 cggcgagcgc cctccgtcag gtcgtagaaa agcccgcttg cgcgggcttt tctggtctgg
    23761 cggaacaatc gcagcgcggg acgctcagcg agtgaacccg ccggtgcgcg gacgcgcctt
    23821 gaacggagcc ttgccgggcg ctgcgccgcc ttcgaccgga cgccgtggcg cgcggtcgaa
    23881 cgggcctgcc gaacgtggcg cgaagctgtc gtcacggcgc ggtgcaaatc caccctcttc
    23941 gcgacgcggt gcgaacgacg gcttgctgaa gcgattgttc gacggcgcac ccgaccggtt
    24001 gccgaagccg ccgggacgac gctcagtgtc ggcacccgga cgcggtgcgt acatgcgtgg
    24061 ctcggggctc ttgggctcca ggccgacgat cgcactgacc gggatgttct gcgtcgtgaa
    24121 ctgctggatg cgacggatca tgccagcgtc catgcgctcg ccgagcgtga tcgccaggcc
    24181 gttcttgccg gcacggcccg tgcggccgat gcggtggacg tagtcctcgg ccttcatcgg
    24241 cagtccgtag ttgatgacgt ggctgatgct cggcacgtcg atgccgcgag cagcgacatc
    24301 ggttgcaacc agcaccttca gctgacgcga gcgcaggcct tgcagcacgc gattgcggcg
    24361 gccctgcggc atgccgccgt gcagcgacgc gaccgcatga cccatctccg ccaggcgatc
    24421 ggccagccag tcggcatcgc gctgcgtgct ggtgaagacc agggcctgtt ccatctcgcg
    24481 ctcggtcagg atgtgatcga gcagcgcgtt cttgtggttg tggttgtcgg cccagtgcag
    24541 acgctgctcg atgttctcgt gcgtgtcggt gtgcgaggac acgtcgatgc gctgcggatc
    24601 acgcaacagg ttctgcgcca ggcggcccac gtgaccggca aaggtggcgc tgaacatcac
    24661 tgtctggcgt gcagccggaa cgtggtcggc gatggtgttg atgtcgtcga tgaagcccat
    24721 gtcgagcatg cggtcggctt cgtcgagcac cagcatctcg acgtgatcga gcacggcctt
    24781 gccggtctgc aggtggtcga gcaggcggcc cggggtcgag atcaggatgt ccagcggacc
    24841 gcgcaatgcc ttgatctgcg ccgggtacgg cacgccaccg acgacggtgg cgacgcgcag
    24901 gccctgcaca tggcgcccgt aggtggaggc ggccttggcc acctgcatcg cgagttcgcg
    24961 ggtcggggtc agcaccagga tgcgcgggcc gtagacggtg gcgcggtcac ggcgctgcgt
    25021 ggtgtcggca cgtgcggcga tgatgcgttc gagcgcgggc aggatgaaag aggcagtctt
    25081 gccgctgccg gtgctcgacg agaccatcag gtccttgccg gcgagggcca gcggaatggc
    25141 cttcatctgc acttcggtgg cggtttcgta gccggcgtcg gcaacggcct tggtcagggc
    25201 ttctgtgagg cccaggcttg caaatgtcat ttcgtacttt caaatgcggt gccgacgctg
    25261 cgcaatgcgc aacgccatga ggcacccgcc ggtttcatga accggcaagg tcaggtcgct
    25321 gggagaacgt tcgctttggg aacagtgaag cggaacagtc aagcgacggc atcgccgccg
    25381 accgttgcct gcaggtaccg tcgaatgcgg taccgactga agcgatatcg agacctgccg
    25441 cactggaaaa gcgtgggctg actcgaatga ggtcagaggg gatgaacgag catcgatcac
    25501 aacaactgcg cagaatttac tgcgttggac cgtcgatgcg agcgaagtcc tagagtttac
    25561 cctgaatccg tgaacgccga aagccccctc gctacgatcc caagacagtt ggcgcatcgc
    25621 ggcctgcacg ttggccgggt atcctgccgc tcccctgcat ccgcccgagt gccgccccac
    25681 tgtcccacat ggccggaatc cacatcaccg acatcgaagc cgccatcaac tggtggcgcg
    25741 agcgctcgcc gtcccccgat ggcatcacgg cctgcagcga ggtccgtgcg ctcgcagagg
    25801 tgtacgcgct gatggtgtac taccgcgaga gcgactgcga cgatggcagc atgcccgccg
    25861 cttcgcacgc cgcctggctg gcctggtatg cgaccacgcc cgacgcgccg tgcatcgcga
    25921 tctgctcgac cagccagggc gacgacatct gcaagggctg cggccgcagc ttcgatgaag
    25981 tgcaggactg gccggtgatg agtccggcag aaaagcgcgc aacgtggcgc cgcatcacga
    26041 tggacggcac cgccctgcgc ttcaaccgct atgccgaacg cgcgatggag gtgcagcgac
    26101 ccgcggcgga cgagtccgcc gcatcgcctt cccgacagcc gggccccgcg tgatcgctga
    26161 gccgtgacgc cgctgcgcag ctggtggtcg agcgcggcgc gcatcctgcc gctggcatgg
    26221 ccggtcttca ttggccagct ggcggtgctg gccttcgcca ccatcgatac gctgatggtg
    26281 gccaggcatt cggccatcga cctggccgcg ctggcggtcg gcaacgccgc ctacgtgtcg
    26341 gtgttcatcg gcttgacagg cgtggtgcag gccgtggcgc ctatcaccgg tcagctgttc
    26401 ggcgcgcgcc agctgccgca ggccggccag caactgcatc aggccctgtg gctggcactc
    26461 ggcctctcgt tcctcggctg cagcctgctg gtctttccgg cgccgttcct ggcactggcc
    26521 caggcaccac cggacgttgc cgaaaaggtc cgcgcgcacc tcggagcgct ggcattcgcc
    26581 ttgccgtcgg ccctgctctt cacggcgttt cgtgggttca acacggcggt atcgcgcccg
    26641 aagatggtga tggcgctgca actcgtcggc ctgacgctga aggtgccgct gaccgcgctg
    26701 ctggtattcg gagccgccct gccgctgccg gcaggttggc cgtggagcga gtggcgcatc
    26761 ccctcgctcg gaaccccggg ctgcggcatc gcgacagcca tcgcgatggc cgtgcaattg
    26821 ctggtcgccg ccgcagtgct gctgcgctcg cccttctacg ctccgttcgg actgcatacg
    26881 cggcgtctgc agcgccccga tccccgcaag ctcgcggccc tgctgcgtct gggcgtgccg
    26941 atgggcctgt cgatcggcat cgaggtcacc ggcttcacct tcatggcctt gttcattgcg
    27001 cggctgggag ccacaccggt ggccggccac cagatcgcgg tcaacctgat ctcggtgatg
    27061 ttcatgctgc cgatctcgct gggcaatgcg accagcacgc tggtggcgca gcgcatcggg
    27121 gccggcgatc cgctcgacgc tcggcggctc ggctggcacg gactgcaact ggccgtgctg
    27181 atcgccgcaa cgctgggcgc atcggtctat gcgctgcgcg atgccatcct gcacgtctac
    27241 acgaacgacg cggtgatcgt cgccgcggcg ctgccattgc tggcgtgggt gatgctgttc
    27301 cacatcgccg acgccgcaca gatccacgtc gcctacacgc tgcgcgcgta ccgcattgcc
    27361 tcggccccgc tgatcatcta cggcatcgcg atctggggcg tcggactggg cggcggctac
    27421 acgctggcgt tcgacgtgac cggattcgcg cctccagcgt tgcggggcgc accaggcttc
    27481 tgggcagcgg cgaccgccgg actgacgctg gccgcgctgg gacttgccgg cttccttgga
    27541 tggatgcagc gcgagactgc ttcgcatcag cgaactccgc gaatcgacgc gcacgactcg
    27601 gcctcttgaa accggacagg tcgcagccat tttgagaggc atggggctcg acggacgggc
    27661 tccgcttcaa gatggagaac gaaccatgta cgactcttac ctgagcgtcc cgagcggatt
    27721 cctttccgac ctcgaccgca tgcgcggcga catcgattcc ctgttcgggt ggggcggcac
    27781 atcgcgtgcg atccgctcga ccggcggcag cacctatcca gccatcaacg tcgggcagag
    27841 ccctgaagcc gtcgatgtct atgtgttcgc gcctggaatc gatgcgagcc gcaccgaagt
    27901 gacactcgat cgcggcgtgc tgacgatttc gggcgagcgc gccagcgatc tgccggccga
    27961 agcacccaag accagcgtcc acggacgcga gcgctacgca ggcaagttcc gccgagcagt
    28021 cgcgctgccg gacgatgtcg atccgaacca ggtcagcgcg acctaccgcg acggcgtgct
    28081 gcgcgtgaat gtccgcaggc gcgaggccgc gcagcccaag cgcatcgcga tcgagtgatc
    28141 tcgcccacac cccgccagac acaggagatt tcgacatgcg cgacacgaaa cccaacaatg
    28201 caagcccggc gcccacacca ttcatggtcc cgcaggtcga cgtggtcgag gacagcagcg
    28261 gcatcacgct gctggccgac ctgcccggcg tcaagaagga cggcctcgac atcaaggtcg
    28321 atggccagac gctgagcctc gagggcacgg tggctctgga cgcaccgcag cccttcgacg
    28381 ccgtcttcgc cgaagtgcgc gcctcgcgct accgccgctc gttcacgctg agcaacgagc
    28441 tcgacagtgc agcgatcgac gcccagttga aggacggcgt gctgcgactg cgcatcccga
    28501 aggttgccga agcgcagcct cgtcgcatcg aggtgcgcgt tggctgaacc cgcccgctcg
    28561 acgggagcgg ccaccctgct gttgatcggt gcggtggccg ccgcgatgtt ctggctcggt
    28621 cagcacagcg cggccgaagc gctggccgcc acgcccccgg cggccagcgt cgtcccgcgg
    28681 gcggttgtcg cgcgcggcga gctggcggcg gacgagcgca acaacatcga tgtcttcaag
    28741 tcggcgtcgc cgtccgtcgt gcacatcacg acgctcgaac gggcggccag cctgttctcg
    28801 atcgatgcga cccaggtgcc gcgcggcacg ggcaccggct tcatgtggga cgaccacggc
    28861 cacatcgtga
//
LOCUS       DAOORV010000032        28622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1910_length_28622_cov_6.270697, whole genome shotgun sequence.
ACCESSION   DAOORV010000032 DAOORV010000000
VERSION     DAOORV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_28622_cov_6.270697"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV56_06340"
     CDS             complement(<1..242)
                     /locus_tag="PKV56_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67468.1"
                     /translation="MRAVLCALAACAVASFSRAAADPAEDASTYVAFVQEFAGNCVSR
                     SGVQILVRNTHPTRAIKIWLDRYHMGIGTGDRSRSEL"
     gene            complement(251..2185)
                     /locus_tag="PKV56_06345"
     CDS             complement(251..2185)
                     /locus_tag="PKV56_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67469.1"
                     /translation="MFSKFEKLLDPYPEAAPTVPPRGLVPFVWACTAGARGLVLGMIL
                     LTASIGVFEAYLFNMLGDIVDWLGRVEPSKLWAQERTHLLLLAAILLGSPLIIALQTL
                     IKHQSLAANLPMRLRWNFHRLMLGQSMQFYQDEFAGRVATKVMQTALATRDVVMILCD
                     IMVFVVIYFVTIIALVGRFDAWLLLPFMGWLVLYCAAIVFFVPRLGQVAKAQADARSL
                     MTGRITDAYTNIATVKLFSHAGRESGYVRSAMQEFIVTVHRQMRLVSGFEVVNHVLSM
                     LLIASTAGATLWLWTRGELGVGAVAAATAMALRLNGISHWVMWEMASLFEHIGTVQDG
                     MNTLSRPHLVTDRADAELMRVSRGEVRFEDVGFAYGGKTAVIDGLTLHIRPGEKIGLV
                     GRSGAGKSTIVNLLLRFYDVESGRVLIDGQDVSRVTQDTLRAQVGMVTQDTSLLHRSV
                     RDNILYGRPEASDVDMVSAAVRAEAHEFIVSLTDPKGRTGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYRLMEGKTVVAIAHRLSTIAAM
                     DRLIVLEHGRIVEEGDHASLLAQGGLYARLWAHQSGGFLGLEVDDSVAVSAESDAPSP
                     AEVALPSPRMEGDVECRTGV"
     gene            2228..2890
                     /locus_tag="PKV56_06350"
     CDS             2228..2890
                     /locus_tag="PKV56_06350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX67470.1"
                     /translation="MTLSPPRPPDSTAPLVIVHVDDQLIVIDKPAGLLSVPGRGDDKA
                     DCAATRVEALFADARVVHRLDMATSGLLLMARGATIQRVLGLAFQAREVTKCYEAIVS
                     GLVECDSGTIDLPLLADWPQRPRQKVDRLHGKPSLTRYRVLHRDPMTCTTRLELQPTT
                     GRTHQLRVHLQAIGHPIVGDSLYAPPDETAAGRLMLHATTLQLQHPTTGQPLTLSSGA
                     PF"
     gene            2959..3732
                     /locus_tag="PKV56_06355"
     CDS             2959..3732
                     /locus_tag="PKV56_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX67471.1"
                     /translation="MSQRLVILTGASRGLGAAMAEQLLAPGTVMLTLSRGRHPTLAAR
                     ASAAQVQIEEWQRDLANSVAVADEVEQWLRGFAASRFDQAILINNAGVIPTVGPADDC
                     NSAELSGALRVGLESTILLTAGFLRATRSWQADRRVLNISSGLGRRAMAGSAVYCAVK
                     AAMDHFSRAVALDESHRAGNSGGSAARIVSLAPGVIDTDMQLQLRTADGAGFPAQDYF
                     RQQHAEGLLASAADSATRIVAYLTRPDFGEEPVADIRTV"
     gene            complement(3763..4509)
                     /locus_tag="PKV56_06360"
     CDS             complement(3763..4509)
                     /locus_tag="PKV56_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HOX67472.1"
                     /translation="MTPSPPWVCAEALPETLASVGHAVLPPLELRLLIGADEAALQAL
                     TPSWADLPPDNFLRDGGRYRRRRHSCFVVTCDEVQQVPHRAHWQPVEYNALHGGIERL
                     FEPMHANIVEQAVWTQLLRGLSAVCSRIKGGQPWFVEAHQFRIDTTDGIGRPTPEGAH
                     RDGVDFVVVALVERSGVKGGETRVFDANGPTGIRFTMTEPWTLLMLDDERVIHETTPI
                     QPLRPDCSDGHRDTLVLTFRRGGFQGPQAA"
     gene            complement(4506..4790)
                     /locus_tag="PKV56_06365"
     CDS             complement(4506..4790)
                     /locus_tag="PKV56_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOX67473.1"
                     /translation="MEWIDFELRGEYIALDALLKATGMTDSGGAAKALVASGRVQVDG
                     RDELRKTCKIRAGQVVAVAGSRIRVAAATATATVPFELPGQDDATPAPDR"
     gene            complement(4803..5297)
                     /locus_tag="PKV56_06370"
     CDS             complement(4803..5297)
                     /locus_tag="PKV56_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX67474.1"
                     /translation="MSAAVNPNESPPSARWRNDGVSVVPGDQLDSNTAQTPGMDRKAA
                     INFARVGAQKLWAGTVHIHPDAKTGAHHHGPLESVIYIIKGRARMRWGDALEFTAEAG
                     PGDFIYVPPFVPHQEINASADETLECVLVRSDGEAVAINLDIEPVEQPTEVRWVDPIH
                     RAPG"
     gene            5543..5752
                     /locus_tag="PKV56_06375"
     CDS             5543..5752
                     /locus_tag="PKV56_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOX67475.1"
                     /translation="MTTQTGTVKWFNESKGYGFIAQDAGGADLFAHFRDITGTGFKTL
                     VENQKVEFEVKQGQKGLQAANIRVL"
     gene            complement(5863..6135)
                     /locus_tag="PKV56_06380"
     CDS             complement(5863..6135)
                     /locus_tag="PKV56_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67476.1"
                     /translation="MSMKKTDLVKNLAKKIDGQMKSAGVPGRFAQGSAAVVDKREQRR
                     LDAEAGLVPFACKLPAVLVKQLHERAAGHEGGMNGLVAGLLQQGLG"
     gene            6262..>6770
                     /locus_tag="PKV56_06385"
     CDS             6262..>6770
                     /locus_tag="PKV56_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOX67477.1"
                     /translation="MALKATIHKASLQLSDMDRNVYGEHALTIARHPSETDERMMIRV
                     LAYALNVPADPRNGALELAKSLWDTDEPDLWQKDLTGQIVHWIDVGQPDDRRILKASP
                     RAERVTVISFSHSTPVWWKGLESRITRARNVAVWQIEAEQSQALAALAQRNMQLQVTV
                     QDGTVWIGEG"
     assembly_gap    6771..6870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7008..7313
                     /locus_tag="PKV56_06390"
     CDS             7008..7313
                     /locus_tag="PKV56_06390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX67478.1"
                     /translation="MKQTRPHTRAADLDIQHNGAECCFECAVDGLRCVIDYRELDGVM
                     QIFHTGVPPALEGRGIAAAMVRFALSHAREHALKIDPQCSYVRVYMQRHPETHDLRV"
     gene            7341..9455
                     /locus_tag="PKV56_06395"
     CDS             7341..9455
                     /locus_tag="PKV56_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOX67479.1"
                     /translation="MNSIDTTSPASLSPPDNDPHDWLEDVLGDRALAWVRERNAESES
                     LLTARPEYAPIRQQLLEVLNSKDRIPSVTRRGHHFYNLWRDDLHPRGLWRRTTLAEYR
                     LAAPAWETVLDLDALAAAEGENWVWGGANALDTEPHRCLVSLSRGGADATVVREFDTA
                     TRQFVEGGFCLPEAKSSTTWIDADTLYVGTDFGPGSLTDSGYPRVIKRWKRGTPLASA
                     DTVFEGEAQDVSASVWLDLTPGHERTIFSRSLDFYHQQHALMNGDQKVAFDIPSDASF
                     SFWNHCADAIDTLLIELRSDWQVGASTYASGSLLATAAAAYLAGERRFDILFQPTATR
                     SLAGFSTTRDHVIVNVLDNVASRLDLWRRTAVGFEARPIDAPFPGTLSVRSLHDPMQA
                     DDPLAECYLLGYTDFLTPDSLFLGHTEPQPGGPALEPLKARPALFDAEGLRTEQRFAI
                     SADGTRVPYFIVWPRGAEANGRNPTLLYGYGGFEVALNPWYSGGYGRAWFERGGALVV
                     ANIRGGGEFGPAWHQAAILENKQRSYDDFIAVAEDLIAQGVTSPRHLGIMGGSNGGLL
                     VGAVFTQRPELFNAVVCQVPLLDMRRYHTLLAGASWMAEYGNPDRQEDWAHIARYSPY
                     QNLRADWKYPEVLFTTSTRDDRVHPAHARKMAARMREMGHALLYYENIEGGHGGAADN
                     SQRAHLQAMEFAYLWRQLGSAS"
     gene            9542..10072
                     /locus_tag="PKV56_06400"
     CDS             9542..10072
                     /locus_tag="PKV56_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67480.1"
                     /translation="MSVALAEAHARTASPIVLDIEASGFGRDSYPIEIGYVLPDGGSY
                     CTLVRPEANWTHWDPEAQRLHRITRATALQHGRPPIDIARHLNRTLSGQTVYSDGWAN
                     DYSWLGTLFDAADLSPSFHIENLRALLSETEAENWHTIKDQVSRELNLRRHRASSDAR
                     LLQMTLMRLRTPTQPR"
     gene            10161..11501
                     /gene="argG"
                     /locus_tag="PKV56_06405"
     CDS             10161..11501
                     /gene="argG"
                     /locus_tag="PKV56_06405"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOX67481.1"
                     /translation="MSTILQHLPAGQKVGIAFSGGLDTSAALHWMRNKGALPYAYTAN
                     LGQPDEPDYDEIPRKAMLYGAEQARLIDCRQQLVAEGIAALQSGAFHISTAGITYFNT
                     TPIGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPALKIYKPWLDQ
                     AFIDELGGRAEMSAFMTKAGFGYKMSAEKAYSTDSNLLGATHEAKDLEHLSSGISIVN
                     PIMGVAFWKDDVAVQRETVSVQFNEGRPVALNGIRYSDAVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQYRDNGRKLGRLLYQG
                     RWFDPQAIMLREAAQRWVARAVTGEVTLELRRGNDYSIMNTVSPNLTYAPERLSMEKV
                     ENAPFSPADRIGQLTMRNLDIVDTRNKLGIYTQAGMLSHSSSTALPGLKNDGSV"
     gene            11566..11877
                     /locus_tag="PKV56_06410"
     CDS             11566..11877
                     /locus_tag="PKV56_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOX67482.1"
                     /translation="MTDQITASIKTRANVYFDGRCVSHSITLPDGTKKSVGVILPSSL
                     TFNTGDPEVMELVEGACRVKLAGSQAWVAYAGGQSFSVPGKSSFDIEVTEALHYICHF
                     G"
     gene            11937..12452
                     /locus_tag="PKV56_06415"
     CDS             11937..12452
                     /locus_tag="PKV56_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67483.1"
                     /translation="MKPSASRLTPLACGALLLACVDAVVAAKPSRLHLVCSGLETVEI
                     IYGTELEPTRKGDFRVEYHIDLDRRAILNVRTGKLTPINVDDREISFGTATDFNLEAR
                     ESSAQRAGDSVASATGVVINRSTGKYFSIATRKQISTEPPPEGSTEPVVTTNVRTRQG
                     TCTDGTPSASF"
     gene            complement(12476..12706)
                     /locus_tag="PKV56_06420"
     CDS             complement(12476..12706)
                     /locus_tag="PKV56_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HOX67484.1"
                     /translation="MSELPALPETPLGRYRHYKGGEYEVVGVARHSETLEPMVVYRPL
                     GNASGLWVRPHAMFFESGDFDGRWQPRFTRLA"
     gene            complement(12720..12998)
                     /locus_tag="PKV56_06425"
     CDS             complement(12720..12998)
                     /locus_tag="PKV56_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67485.1"
                     /translation="MAKAALISAVLASAVTAAAFLLNPSPEQHRAQIREAIAQRSPLA
                     GALGVGVFTAFTSTYHPLGVASYTTVNERTVSIGAFGVVHVLSLSQDE"
     gene            13221..16565
                     /locus_tag="PKV56_06430"
     CDS             13221..16565
                     /locus_tag="PKV56_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOX67486.1"
                     /translation="MKGCVARHGPGPFPPRHSTRLPNPVPALPAYAELHCRSNFSFLT
                     GASHPEELVARAAHLGYGALAITDECSLSGVVRAHAEARRCALHLIVGAEMSVTRAPA
                     EVGGGAPLPAMRLVLLAQTRAGYGNLSQWITLARRRAGKGCYLAHRFDLEASSEAHRD
                     NALAGLPGCLALLVPDVTQPVEQMLADARWLHSVFGDRATLAVELLLRARDDVLIDRV
                     REVSAATGLPVVAAGDVLMHVRSRKPLQDTLSATRLGLPLAQCGSQLEPNAEQHLRPI
                     GRLQALHEPAWLAQTMALAARCTFSLDELRYEYPQEIVPPGTTPAGHLRALTESGALK
                     RFPPEAFPQGLPPTVRAQIEHELALIAQLQYEPYFLTVADIVQWARSQGILCQGRGSA
                     ANSTVCYCLHVTEVDPTRQHTLFERFISAERNEPPDIDIDFEHQRREEVIQYIYGKYG
                     RDRAALTAVVISYRPRSALRDVGRALGLDADRIHEVARSQQWFDGRRIDPERLREKGY
                     DLSSHVTRLWIELTEQLIGFPRHLSQHPGGFVISRGPLAQLVPIENAAMPDRSVIQWD
                     KDDLDALGLLKVDILALGMLTALRRSLDLISHKLGRRFALQDVPPEDAATYDMICRAD
                     TVGVFQIESRAQMSMLPRLKPRVFYDLVIEVAIVRPGPIQGGMVHPYLRRRDGIEPET
                     YPNPEIAEALSRTKGVPIFQEQVMQLAVLAADFTPGEADSLRRAMAAWKRKGGLGPFH
                     ERLVGRMVEKGYQREYAENIFRQIQGFGEYGFPESHAASFALLAYASSWIKRHHPDAF
                     LAALLDSQPMGFYAPAQLVRDAREHGVDVRPVDVRFSRWNSSLEPPGPDDAALHPVRL
                     GFNRIGGLSQAAAERIVQAREADGAFIHLEDLTRRAALDARDLNLLAASDALRGLVAG
                     SPEPHASAPSRSPRGHRHQAAWAVAAIDTRPSALLHGMRSREPATEAFPITLPSPSAA
                     EDMLADYRSTGLTLNRHPLALLRPQLDAFRIETAAVLRTYPDGRIARASGLVTHRQRP
                     GTAKGTVFVTLEDDTGTVNVIVWPTVAQAQRQPLIGARLLTVYGQWQRSTDVPGEHAV
                     MHLLASRLIDHTALLNGLVTRSRDFH"
     gene            16651..17022
                     /gene="ssrA"
                     /locus_tag="PKV56_06435"
     tmRNA           16651..17022
                     /gene="ssrA"
                     /locus_tag="PKV56_06435"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            17196..18497
                     /locus_tag="PKV56_06440"
     CDS             17196..18497
                     /locus_tag="PKV56_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOX67487.1"
                     /translation="MATNILSDKTIRAALKKATEAGKPSTINDGDGLSLIARPDGAGW
                     WRLRYWLDSRENRLSLGTYPDVSLADARGRRDAARKQIAAGTDPSEARKADKAAKQKE
                     RESQALADAGLPGPGTFEHVAREWLTTVHEAKVSAGHAERTRIRLEQDAFPWIGRRPI
                     ADIEAPALLECLRRVEARGAIETAHRVKDACGQVFRYGIAIGQCKRNPASELRDAMRP
                     VVSRHHAAIVDPVEAGKLLSDMLAYEGHPVTRAALQLSALLMLRPGELRHMEWAWIDF
                     EESTLTMPAEVMKRKKADKADGPPHLVPLAQQAVAILKDLQPLTGGERRRFVFPSLLT
                     GERCMSENTVRGALRRMGYANDEMTAHGFRAMARTMIAERLEIAPEVIEAQLAHAVGD
                     SLGRSYNRTQFLDQRREMMSKWADYLDRIRIGAEVLPIKAA"
     gene            18743..19225
                     /locus_tag="PKV56_06445"
     CDS             18743..19225
                     /locus_tag="PKV56_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67488.1"
                     /translation="MDKALTEEDRTTAIGLARYAYDYIGAAMLVERNDPMPTHVSPVP
                     AYFLALHGIELTLKSYLRHKGVTAKELRGQKYGHDLHACHKKAKELGLLSIFKEQAND
                     ADAMLMLVGLNEHQGLRYIKTGMKRFPLWSLVEPLSVRLHQAVAPQVGYKSFDITYHQ
                     "
     gene            19222..19995
                     /locus_tag="PKV56_06450"
     CDS             19222..19995
                     /locus_tag="PKV56_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67489.1"
                     /translation="MNPAARLHRIYDKLVSQGNDYQLLQTWADVFDIDKSGPHYEDDV
                     TACVMALRSEIALTRKRLDEIGAPANLTSPGFERLREVAAPGLLHGKWHSHRGNISPP
                     ECRKVFEWAEWALREDAEPDMPAEDMNELRAELESLEAALNDAEVSPYLRDFIQRQVD
                     TIRAALRVYGVQGARTLQDALQKVAGAYTVERARVEAEHGTAPEATKGLFAKASGVIK
                     KTADVCDNLGKIGKFGEQAWTLAGTVGPLVLPYLPKLGG"
     gene            20318..20728
                     /locus_tag="PKV56_06455"
     CDS             20318..20728
                     /locus_tag="PKV56_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOX67490.1"
                     /translation="MTNRTTPPSTAATRTAGNVVPIARPTVRPPLPPADDPHALIDIR
                     GVQTLTCMSESWLHDAIRRGEFCPPLRLGPRCSRWRIADVRAWLIKQFEAASADTSTA
                     DLVRARAKKGADAAARHRAAKKHRPDCTDGGGSS"
     gene            20897..21862
                     /locus_tag="PKV56_06460"
     CDS             20897..21862
                     /locus_tag="PKV56_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOX67491.1"
                     /translation="MTDSFGSFRTELLATLGGAPATIEPGRLHRFSTSERRGDSAGWC
                     KLFDDLRGGVYGCHRQFPGESFTWSAIDRRTMTLEQRSLLARQFEAATAEREAQQRMQ
                     WSENAQRIAQMWAQCVPISPDDPCSAYLTQRGLVGAWPLPEVLRFHPSLPYWNGVEKL
                     GPFPAMVAPLIAPDGRTVALHRTYLTAHGRKAGVPTPKKVTGASGVLAGASIPLHEPT
                     GGVIGIAEGIETALAAWCISGVPTVAAYCAGNLAAWQCPAGVQRLVVFADADKAGREA
                     ADTLRAKALSRHLRCEVMTPTDEGSDWCDVWAASRPTSINAEVAA"
     gene            21859..23283
                     /locus_tag="PKV56_06465"
     CDS             21859..23283
                     /locus_tag="PKV56_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOX67492.1"
                     /translation="MSAVVQDLTRVARLAESLAVDRAEAIKRARTALAPSLAGRQAPA
                     YPLAALGPLAFACEAIAGAGQVQPAMVGQCLLGAASLLTQGLFNVETLNGKRPLSLYL
                     LTLGDSGEGKSTAQGAALAPVNDWQSKAAKHYGEALKDFDKAKSSRKRADDPPEPPAC
                     PYRLVGDATVEGLRRDLDTGVCSQGVFSDEAAAILSGYGMSSDHRSKTAGVFSKLWDN
                     GHLSVSRVTGGRVERYGRRVALHWLIQPTAASESIGDPLLSALGFWPRFLAAWPDAQA
                     PRLARSFRPDTLPAVGAYWQRCNGLLNEALPDDAGDCPVLPLTEDARAHLSSAFERFE
                     RDARRGELQVVKPFALRASEQACRIAGVLSAFEGASSVSADAMHGALALVAYSLKTWQ
                     TIVDEGAADQRGAIALRLYEWLTNRPGWCEPLAVIVNKGPACVRTRDRRDAALAELEE
                     AGLVQVIEGKAQALIGQAIDGATP"
     gene            23295..23891
                     /locus_tag="PKV56_06470"
     CDS             23295..23891
                     /locus_tag="PKV56_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67493.1"
                     /translation="MDELLAKFASGTVLAIAPAARAKAANSAKSEHPRGQDPALRVCE
                     GLRTSANAVGIGRRDDADSQNFADIRKPERGPHIEESCGSSQDSQDSQGHPVQCIADI
                     ARDSTAIGWTDAEVARFLDRRARLMRWGWPELEAEALADRLLRRDQDHDDRVSCADCK
                     HYRPGRCGNHRRAGLNVADIGRDLATLLQRCPGFDPIG"
     gene            23896..24372
                     /locus_tag="PKV56_06475"
     CDS             23896..24372
                     /locus_tag="PKV56_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67494.1"
                     /translation="MTRKNPDSTLIASDRLTSKEENFARLRAAGTPQGDAYRQAYECD
                     GSTTRTVAVESSRVESKPRVKRRILELRDAHASAVVSYSVAAAMSELDESMALAKAKE
                     SPMAMIKVVEVRMKLYGLGIGEAKNQADKDEMTPEELEAALADVRALRAAQAASHH"
     gene            complement(24612..25379)
                     /locus_tag="PKV56_06480"
     CDS             complement(24612..25379)
                     /locus_tag="PKV56_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67495.1"
                     /translation="MFDMQAATSQTFEQHFLTFARLFADSSLQLLNQKLTANLDIERF
                     LEASSRSQGSMVGSARLAWPENADEVLGLQWLLVQKFAREPRDLLNFAHTYYTVGRNL
                     TGELHSVTRQLLIPFARDYKDYVMTIDRSEVAASLSGVADGVGNAPAHITYHINGNNA
                     RVNHQSIDNSVNAVTINSTVMNCIRELRDEIQRAPLSEVQRGSAVEVVDEVEAQITSG
                     KPKHAILAALLASLPAMESITSIGNSLLEALKTTGLM"
     gene            complement(25411..25704)
                     /locus_tag="PKV56_06485"
     CDS             complement(25411..25704)
                     /locus_tag="PKV56_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014859395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67496.1"
                     /translation="MAFLKGLDDLQRTLAEAQIALEAVNGELGTLSFNPEDPGSVEAA
                     IAESERLVDERLGKYANNSIVGPLIVQMKDSFRTAVIEKAAEARLTGEASDGE"
     gene            26438..27055
                     /locus_tag="PKV56_06490"
     CDS             26438..27055
                     /locus_tag="PKV56_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67497.1"
                     /translation="MSTHPMTPERFEQFARQPARTREELETMRANALAKGNVELAHIA
                     AEVLAERFPTQTKQRSGPTQTTAAFLGRSETFGTGKEAYLWLLERFRQHQPLIFEAYE
                     LLRHKDRSAGTRVAKDPALLFPRGSSRIGNPANYATLTGGWYADTNLNHQDKFSTLVR
                     FAHLAKLEYPEDWDFRVEGGTMELRKQQDLVIQVRELLRELGLAN"
     gene            27098..27580
                     /locus_tag="PKV56_06495"
     CDS             27098..27580
                     /locus_tag="PKV56_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOX67498.1"
                     /translation="MDKFLLQQQVLERLAEDLLQAEQAVRAAHETATHEENIAENKYD
                     TLGLEAAYLATGQARRAEAIRQAMAHWREFRPSPYDASKGIQLGALVCLVDSDDKQQQ
                     LFLGPDGGSMKLVSGAQVVQVVSSEAPLGRAMLGKFEGDEVSIQVAPIRQQFEVLRVH
                     "
     gene            28242..>28622
                     /locus_tag="PKV56_06500"
     CDS             28242..>28622
                     /locus_tag="PKV56_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67499.1"
                     /translation="MEGLARHESLIQTLALVLAYLAVVLWERLRPLLRYDADDRWRLA
                     GNFGLLVVNHAVPFLVVPVVAALGSWAASGLNLGWLNAVHAPRWLMFMVAVVALDLTG
                     WLVHWSMHKVGPLWRVHRVHHSDLR"
BASE COUNT         5270 a         9476 c         9004 g         4772 t
ORIGIN      
        1 agctcggagc gggagcggtc gcccgttccg atacccatgt gatagcggtc gagccagatc
       61 ttgatggccc tcgtcggatg cgtgttcctg accaggatct gcacgccgct gcgcgacacg
      121 cagttgccgg cgaattcctg cacgaacgcg acgtaggtac tggcgtcttc cgccgggtcc
      181 gccgccgccc tcgaaaacga tgcaacagcg caggcagcca aagcgcagag gacggcgcgc
      241 atccggcgta tcacacacct gttcggcact cgacatctcc ttccattcgg gggctaggca
      301 acgccacctc cgcaggcgac ggcgcatcgg attcggctga aaccgccacg ctgtcatcga
      361 cctccaggcc gaggaaccca ccgctctggt gagcccacag gcgcgcgtac agccccccct
      421 gggccagcag gctggcgtga tcgccctctt cgacgatgcg tccgtgctcg agcacgatca
      481 ggcggtccat tgcagcgatg gtcgacaacc ggtgggcgat ggccaccacc gtcttgcctt
      541 ccatcagcct gtaaaggctg gcctggatcg ccgcctcgac ttcgctgtcg agcgcgctgg
      601 tggcctcgtc gagcagcagg atcggcgcgt ccttcagcat cacgcgggca atcgcgatgc
      661 gctggcgctg gccgccgctg agcttgacgc cgcgctcgcc cacgtgagcg tcgtacccgg
      721 tgcgcccctt ggggtcggtg agcgagacga tgaactcgtg cgcttcggca cgcaccgcgg
      781 ccgacaccat gtcgacatcg ctggcttcgg gacggccata gaggatgttg tcgcgcacag
      841 aacgatgcag cagcgatgtg tcctgcgtca ccatgccgac ctgcgcacgc agcgtgtcct
      901 gcgtgacccg cgaaacgtcc tgcccgtcga tcagcactcg cccagactcg acatcgtaga
      961 agcgcaacaa caggttgacg atcgtgctct tgcccgcgcc cgagcgaccg accagaccga
     1021 tcttctcgcc cggccggatg tgcagcgtca gcccgtcgat cacggccgtc ttgccgccat
     1081 acgcaaagcc cacgtcctcg aaccggacct cgccacggga gacccgcatc agttcagcgt
     1141 cggctcggtc ggtgaccaga tgcggccgcg acagcgtgtt catgccgtcc tgcaccgtgc
     1201 cgatgtgctc gaagagtgaa gccatctccc acatgaccca gtgcgatatg ccgttgagcc
     1261 gcagcgccat cgcggtcgct gcggccaccg caccgacacc gagctctccg cgcgtccaca
     1321 accacagcgt ggcgccggcc gtgctcgcga tcagcagcat gctgagcacg tggttcacga
     1381 cttcgaagcc gctgaccagc cgcatctgcc gatgcaccgt cacgatgaac tcctgcatgg
     1441 ccgagcgcac gtaacctgac tcgcgtccgg catgcgagaa cagcttgacg gtcgcgatgt
     1501 tggtatacgc gtcggtgatg cgcccggtca tcagcgagcg cgcatcggcc tgcgccttgg
     1561 ctacctgccc aaggcgcgga acgaagaaga cgatggccgc gcagtagagc accagccagc
     1621 ccatgaacgg cagcagcagc cacgcatcga atcgaccgac cagcgcgatg atggtgacga
     1681 aatagatcac cacgaagacc atgatgtcgc acaggatcat caccacgtcc cgcgttgcga
     1741 gtgcggtctg catgaccttg gtcgccacgc gtccggcgaa ctcgtcctga tagaactgca
     1801 tgctctggcc gagcatcagc cggtggaagt tccagcgcag ccgcatgggc aggttggccg
     1861 ccagcgactg gtgcttgatc aaggtctgca gcgcgatgat caacgggctg cccagcagga
     1921 tggctgcgag caggagcaga tgggtccgct cctgggccca cagcttcgat ggctcgacac
     1981 gacccagcca gtcgacgatg tcgcccagca tgttgaaaag gtaggcttcg aaaaccccga
     2041 tcgacgcggt gagcaggatc attccgagca ccagtccgcg ggcaccggcg gtacaggccc
     2101 agacgaacgg caccagaccc cgaggcggca cggtcggcgc cgcctcggga tagggatcca
     2161 gcaatttctc gaacttcgaa aacatcggcc ggagcgtagc cgatgacatc atccgcgctt
     2221 gccacacatg accctctccc ctcctcgtcc gccggattcg acggctccgc tggtcatcgt
     2281 gcacgtcgat gaccaactga tcgtcatcga caagcctgcc gggctgctgt cagtgccggg
     2341 ccgcggggac gacaaggccg attgcgccgc gacgcgggta gaggcgctat tcgccgatgc
     2401 acgggtcgtg caccggctgg acatggcaac ctcgggtttg ctgctgatgg cgcgcggcgc
     2461 cacgatccag cgcgttctcg gcctggcctt ccaggcccgg gaagtgacca agtgctacga
     2521 ggccatcgtc agcggactgg tggagtgcga cagcggcacg atcgacctgc cgctgctcgc
     2581 cgactggcca cagcgcccgc gacagaaggt cgaccggctg catggcaagc cttcgctgac
     2641 ccgctaccgc gttctgcacc gcgatccgat gacgtgcacc acgcgactcg aacttcagcc
     2701 gaccacgggc cgcacccacc agctgcgggt tcatctgcag gcgatcgggc atccgatcgt
     2761 gggcgactcg ctgtacgccc cgcccgacga aactgcggcc ggacggttga tgctgcacgc
     2821 caccacgctg cagctgcagc atccgaccac cgggcagcca ttgaccctct cgagcggggc
     2881 gccattctga gccggatgcc cggcagtccg aatcagtcca ccgaaaattc aagttcagca
     2941 gcgaatcgga gcccacccat gtcccagcga cttgtcatcc tcaccggcgc atcgcgcgga
     3001 ctgggcgcgg caatggccga gcagttgctg gcgcctggca ccgtgatgct gaccctgtcg
     3061 cggggccgcc acccgacgct ggccgcgcgt gcctcggcag cgcaggtcca gatcgaggaa
     3121 tggcagcgcg atctggccaa cagcgtggcc gtcgccgatg aagtcgagca gtggctgcgc
     3181 ggcttcgctg cgagccgctt cgaccaggcc atcctgatca acaacgcggg cgtgatcccg
     3241 acagtcggcc ccgccgacga ctgcaattcg gcagagctgt cgggcgcact gcgcgtcggg
     3301 ctcgaatcca ccatcctgct gaccgcgggg ttcctgcgcg ccacccgttc ctggcaggcc
     3361 gacagacggg tgctgaatat ctcttcgggc ctcggccgtc gcgcgatggc gggcagcgcg
     3421 gtgtactgcg cggtcaaggc agccatggat catttctcgc gcgcggtggc actcgacgaa
     3481 tcgcaccgcg ccggcaactc gggcggctcg gccgcgcgca tcgtttcgct ggcaccgggc
     3541 gtgatcgaca ccgacatgca attgcaactg cgaacggccg acggcgcagg atttcctgct
     3601 caggactact tcaggcaaca gcacgcggaa gggctgctgg cgagcgccgc agacagcgcg
     3661 acgcggatcg tggcatacct gacccgcccg gatttcggtg aggaacctgt cgcggatatc
     3721 cgaacggtct gaagcgctgc cgtctgcgtg aaccgtgctg cctcaggcag cttgcggccc
     3781 ctggaatccg ccgcgccgga aggtcagcac gagcgtgtcg cggtgtccat cggaacagtc
     3841 gggccgcaga ggctggatcg gcgtggtttc gtggatcacg cgctcgtcgt cgagcatcag
     3901 cagtgtccaa ggctcggtca tcgtgaagcg gatgcctgtc ggcccgttcg catcgaacac
     3961 gcgtgtctcg ccgcctttca cgccactgcg ttcgacgagt gcgaccacga cgaaatcaac
     4021 gccatcgcgg tgcgcaccct cgggcgtcgg ccgtccgatg ccgtcggtgg tgtcgatgcg
     4081 gaactggtgc gcctcgacga accacggctg cccgcccttg atgcgggagc acaccgccga
     4141 cagaccgcga agcaactgtg tccacacggc ctgttcaacg atatttgcgt gcatcggctc
     4201 gaacagtcgc tcgataccgc cgtgcaacgc gttgtattcg acgggctgcc agtgggcgcg
     4261 atgagggacc tgctgcactt catcgcatgt caccacaaag catgaatgcc ggcgacggcg
     4321 atagcggccg ccgtcacgca ggaagttgtc tggcggcagg tcagcccacg acggggtcag
     4381 ggcctgcaga gccgcctcgt cggcaccgat cagcaaccgc aactcgagcg gcggcagcac
     4441 cgcgtgaccg accgatgcca gggtctcggg caacgcttcg gcacagaccc aggggggcga
     4501 cggggtcatc gatcaggcgc cggagttgca tcgtcctgac ccggcagttc gaacggcacg
     4561 gtcgcggtcg cggttgcggc cgcgacccgg attcggctgc cagccacggc cacaacctgg
     4621 cccgcgcgga tcttgcaggt cttgcggagt tcgtcgcggc cgtcgacctg cacccggccc
     4681 gacgccacca gagccttggc cgcaccgcca ctgtccgtca tgccggtggc cttcagcagg
     4741 gcatcgagcg cgatgtattc accgcgcaac tcgaaatcga tccattccat cgggtgctga
     4801 cgtcagccgg gcgcacggtg gatcggatcg acccagcgca cttcggtggg ttgctcgacc
     4861 ggttcgatgt cgaggttgat cgccaccgcc tctccatcgc tgcgcaccag cacgcattcg
     4921 agcgtctcgt ccgcgctggc gttgatctcc tggtgtggaa cgaaaggcgg cacgtagatg
     4981 aaatcgcccg ggccggcttc ggcggtgaac tcgagcgcgt cgccccagcg catgcgcgca
     5041 cggcccttga tgatgtagat cacgctctcc agcgggccat gatgatgggc gccggtcttt
     5101 gcatcggggt ggatgtgcac agtgccggcc cagagcttct gcgcgccaac ccgcgcgaaa
     5161 ttgatggctg ccttgcgatc catgcccggc gtctgtgccg tgttcgaatc aagctggtcg
     5221 cccgggacga cgctcacgcc atcgtttctc catcgagccg agggtgggga ttcgttcggg
     5281 ttcacggcgg cggacacgtt gttgcaacga tgccattctc ggccatcgct gcgtcgctct
     5341 tatcgagact tcgcacgacg cccctcgcta tggggtacat tactcgaaca ggtatacgca
     5401 agaccgtggt tgctagtgat ctcatctcac ctgcttctgt tctgaagcgg gatcgcaaga
     5461 aatccccagt tcgatcttga gtgtctctgc atgcggcagc atgtgcagcc gcatcatttt
     5521 gttgatagat aggaatacgt ccatgacgac gcaaacaggt accgtgaagt ggttcaacga
     5581 gagcaagggc tacggcttca tcgctcaaga tgcaggcggc gctgacctct tcgcccactt
     5641 ccgtgacatc acgggcaccg gcttcaagac cctggtcgaa aaccagaagg tcgagtttga
     5701 agtcaagcaa ggccagaagg gcctgcaagc tgccaacatc cgcgtgctgt aaagcaacgc
     5761 gagttcagct ccttcaagac gcggcttcgg ccgcgttttt tattgcccgg acgcgaatgc
     5821 gcgcaacctg cccggatccg ggtcgcggcc cggccgaccg gatcagccca agccctgttg
     5881 caacaggccg gccaccaatc cgttcatgcc accctcgtgc ccggccgcgc gctcgtgcag
     5941 ttgcttgacc aacacggcgg gcagcttgca ggcgaacggc accaggccgg cttcggcgtc
     6001 gagccgacgc tgctcgcgct tgtcgaccac tgcggccgaa ccctgcgcaa agcgcccggg
     6061 cacgcctgct gacttcatct gcccgtcgat cttcttggcc aggttcttga cgagatcggt
     6121 cttcttcatg ctcatgatga ggtgcgcggc gtgcgtgcga atttttgagg atccgacatt
     6181 gtcacgccgc acatcgaagt cgaaccacag gtttgcagcg tgcccgacaa tcgaaggctg
     6241 cacgccaacc gggacgcatc catggcattg aaggccacca tccacaaagc cagcctgcag
     6301 ctcagcgaca tggaccgcaa cgtctacggc gagcacgcgc tgaccatcgc acgccacccg
     6361 tcggaaaccg acgaacgcat gatgatccgg gtgctggcgt acgcgctgaa cgtgcccgcg
     6421 gacccgcgga acggcgcact ggaactcgcg aagagcctgt gggacaccga cgagcccgac
     6481 ctgtggcaga aggacctcac cggccagatc gtgcactgga tcgacgtcgg ccagcccgac
     6541 gacaggcgca tcctgaaggc cagcccccgc gccgaacggg tgacggtcat cagtttcagc
     6601 cacagcacgc cggtctggtg gaagggcctc gagagccgga tcacccgcgc gcgcaacgtg
     6661 gcggtctggc agatcgaggc cgagcagagt caggcgctgg ccgcgctggc gcaacgcaac
     6721 atgcagttgc aggtgacggt ccaggacggc acggtctgga tcggcgaggg nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaacatgca gttgcaggtg acggtccagg
     6901 acggcacggt ctggatcggc gagggcgatc gatcggtcga ggtgcggccg gttcatctga
     6961 atgcacagta ggcacgggat gattccatcg ggcccagaat cggtcggatg aaacagaccc
     7021 gaccccacac gcgagcagcc gacctagaca tccagcacaa cggcgcggag tgctgcttcg
     7081 agtgcgcagt cgatggcctg cgctgcgtca tcgattaccg tgaactggac ggtgtcatgc
     7141 agatcttcca caccggggtg ccgccggcgc tggaaggtcg aggcatcgcc gcggcgatgg
     7201 tgcgattcgc gctgtcgcac gctcgggagc acgcactgaa gatcgatccg cagtgctctt
     7261 acgtgagggt ctacatgcag aggcacccgg aaacacacga ccttcgcgtc tgaccttcaa
     7321 cttccgtcga cacgccctgc atgaactcca ttgacacgac atcgcccgca tccctcagcc
     7381 cccctgacaa cgatccgcac gactggcttg aagacgtcct gggcgaccgc gcactcgcct
     7441 gggtgcgcga gcgaaacgcc gaaagcgaat cgctgctgac cgcccgcccc gagtacgcgc
     7501 cgatccgcca gcagctgctg gaagtgctga actcgaagga ccgcatccct tcggtgacgc
     7561 gacgcggcca ccatttctac aacctgtggc gcgatgacct gcatccgcgc ggcctgtggc
     7621 gccggacgac gctggccgaa tacagactcg cagcgccagc ctgggagacc gtgctcgacc
     7681 tcgatgcgct ggcagcggcc gaaggcgaga actgggtgtg gggcggcgcc aacgcgctcg
     7741 acaccgagcc gcatcgctgt ctggtctcgc tgtcgcgcgg cggcgccgat gcgaccgtgg
     7801 tgcgcgagtt cgacaccgcc accaggcagt tcgtggaggg tggcttctgc ttgcccgagg
     7861 ccaagtcgtc gacgacctgg atcgatgccg acacgctgta cgtcggcacc gatttcggtc
     7921 cgggcagcct gaccgactca ggctacccgc gggtgatcaa gcggtggaag cgcggcaccc
     7981 cgctggcatc ggccgacacg gtgttcgaag gcgaggcgca ggacgtgtcc gccagcgtct
     8041 ggctggacct gacgccgggc cacgagcgca ccatcttcag ccgttcgctc gacttctatc
     8101 accagcagca cgccctgatg aatggcgatc agaaggtcgc gttcgacatt ccgagcgacg
     8161 cgtcgttcag cttctggaac cactgcgcgg acgcgatcga cacgctgcta atcgagttgc
     8221 gcagcgactg gcaggtgggc gcgtcgacct acgccagcgg ttcgctgctg gccaccgctg
     8281 cggcggcgta cctggctggc gagcgcaggt tcgacatcct gttccagccg accgccaccc
     8341 ggtcgctggc aggcttctcg acaacgcgcg atcacgtcat cgtcaacgtg ctcgacaacg
     8401 tcgcgagccg gctcgatctc tggcgtcgca ccgcagtggg cttcgaggcc aggccgatcg
     8461 atgcgccgtt tccaggcacg ctgagcgtgc gcagcctgca cgacccgatg caggccgacg
     8521 atccgctggc cgagtgctac ctgctgggct acaccgactt cctgacaccg gattcgctgt
     8581 tcctcggaca caccgagccg cagccgggcg ggcccgcgct cgaacccctg aaggctcgcc
     8641 ccgcgctgtt cgacgcggaa ggcctgcgca ccgagcagcg ctttgccata tcggccgatg
     8701 gcacccgcgt gccctacttc atcgtctggc cgcgcggcgc cgaagccaac ggtcggaatc
     8761 cgaccttgct ttacggctac ggcggattcg aggtcgcgct gaacccctgg tactcgggag
     8821 gctacggccg cgcctggttc gagcgcggcg gcgcgctggt ggtggccaac atccgcggcg
     8881 ggggcgagtt cggccccgcc tggcaccagg ccgcgatcct ggaaaacaag cagcgcagct
     8941 acgacgactt catcgccgtg gccgaggacc tgatcgcaca gggtgtcacc agcccccggc
     9001 atctaggcat catgggcggc agcaacggcg ggctgctggt cggcgcggtc ttcacgcagc
     9061 ggcccgagct gttcaacgcg gtcgtctgcc aggtgccgct gctcgacatg cgccgctacc
     9121 acacgttgct ggccggggcg tcgtggatgg ccgaatacgg caacccagac cggcaggaag
     9181 actgggccca catcgcgcgc tacagcccgt accagaacct gcgagccgac tggaaatacc
     9241 ccgaggtgct gttcaccaca tccacgcggg atgaccgcgt gcatcccgcc catgcccgca
     9301 agatggcggc gcgcatgcgc gagatgggcc acgccctgct gtactacgag aacatcgagg
     9361 gcggccacgg tggcgcggcc gacaattccc agcgggcgca cctccaggcg atggaattcg
     9421 cctacctgtg gcgccagctc ggcagcgcca gctgacttgt cgtgaggcat tcctcgctgc
     9481 gcaggcccgt ctaggtcttc ctcctctggt ggctgtcacg atacgtgctt aggcttgtta
     9541 catgtccgtc gcactggccg aagcacacgc ccgcaccgcg tccccgatcg tcctcgacat
     9601 cgaggcctcg ggattcggtc gcgacagcta ccctatcgag atcgggtacg tgctgcccga
     9661 tggcggcagc tattgcaccc tggtccggcc cgaggccaac tggacccact gggaccccga
     9721 ggcgcagcgc ctgcaccgca tcacgcgcgc caccgccctg cagcacggcc gcccgccgat
     9781 cgacattgcc cgacatctca accgcacact gtccggtcag accgtctaca gcgatggctg
     9841 ggccaacgac tacagctggc tcggcacgct gttcgacgcc gccgatctgt cgccgtcgtt
     9901 ccatatcgag aacctgcgcg cgctgctcag cgagaccgag gcggaaaact ggcacacgat
     9961 caaggatcag gtgagccgcg agctgaacct gcgccggcat cgtgccagtt cggacgcccg
    10021 actgctgcag atgacgctga tgcggctgcg cactccaacg caaccgcgtt gacagcggcg
    10081 agaatacgag ctgtcgcgcg gccccggccg cctgttcacg cccgtcccgc gcagccgctt
    10141 cactcacctc tgttcagacc atgtccacca tccttcagca tcttcccgcc ggccagaagg
    10201 tcggcatcgc gttctccggc ggtctggaca ccagcgccgc gctgcactgg atgcgcaaca
    10261 agggggccct gccctacgcc tacaccgcca acttgggcca gcccgacgag cccgactacg
    10321 acgagatccc gcgcaaggcg atgctctacg gcgccgagca ggcgaggttg atcgactgcc
    10381 gccagcagct ggtcgccgaa ggcatcgccg cgctgcagag cggcgcgttc cacatcagca
    10441 ccgccggcat cacctacttc aacaccacgc cgatcggccg cgccgtcacc ggcacgatgc
    10501 tggtggcggc catgaaggag gacgacgtca acatctgggg cgacggcagc accttcaagg
    10561 gcaacgacat cgagcgcttc taccgctacg gcctgctgac caacccggcg ctgaagatct
    10621 acaagccctg gctcgaccag gccttcatcg acgagctggg cggtcgcgcc gagatgagcg
    10681 ccttcatgac gaaggccggc ttcggctaca agatgagcgc cgagaaggcc tactcgaccg
    10741 actccaacct gctcggcgcc acgcacgagg ccaaggatct cgagcacctc agcagcggca
    10801 tcagcatcgt caacccgatc atgggcgtgg ccttctggaa ggatgacgtc gcggtccagc
    10861 gcgagaccgt cagcgtgcag ttcaacgaag gccgcccggt ggcgctcaac ggcatccggt
    10921 acagcgacgc ggtggaactg atgctcgagg ccaaccgcat cggcgggcgc cacggactgg
    10981 gcatgagcga ccagatcgag aaccgcatca tcgaggccaa gagccgcggc atctacgagg
    11041 caccgggact ggcgctgctg ttcatcgcct atgagcggct ggtcaccggc atccacaacg
    11101 aggacacgat cgagcagtac cgcgacaacg ggcgcaagct cggccgcctg ctgtaccagg
    11161 gccgctggtt cgatccgcag gccatcatgc tgcgcgaggc cgcgcaacgc tgggtggcgc
    11221 gtgcggtcac cggcgaggtc acgctcgaac tgcgccgcgg caacgactac tcgatcatga
    11281 acacggtctc gccgaacctg acctacgcac ccgagcgcct gagcatggag aaggtcgaga
    11341 acgcgccctt ctccccagcc gatcgcatcg gccagctgac gatgcgcaac ctcgacatcg
    11401 tcgacacccg caacaagctg ggcatctaca cgcaggccgg catgctgtcc cacagcagca
    11461 gcacggccct gcccggcctc aagaacgacg gcagcgtctg aaccacgaat ccgccgggac
    11521 ctgcctctcg aaccgcaccc gtacacgcac cagcctctca tcaccatgac cgaccagatc
    11581 accgcctcca tcaagacccg cgccaatgtc tacttcgacg gccgctgcgt ttcgcacagc
    11641 atcacgctgc ctgacggcac gaagaagagc gtcggcgtca tcctgccttc cagcctgacc
    11701 ttcaacaccg gcgatcccga ggtgatggaa ctggtcgaag gcgcctgccg cgtcaagctg
    11761 gcgggcagcc aggcctgggt ggcctacgca ggcggacagt cgttcagcgt gcctggcaaa
    11821 tcgtccttcg acatcgaagt caccgaagca ctgcactaca tctgccattt cggttgagcc
    11881 tgggggccgc ggattcgtcg cggcgacacg cgcggactgc atcaggagca ctgcacatga
    11941 agccatcggc ctcgcggctc accccactgg cctgcggcgc actgctgctg gcttgcgtcg
    12001 acgctgtagt ggccgccaag ccgtcgcggc tgcacctcgt ttgcagcggc ctggaaaccg
    12061 tcgagatcat ctacggcacc gagctggagc cgacccgaaa aggtgatttc cgggtcgagt
    12121 accacatcga cctggaccgc cgggccatcc tgaacgtccg caccggcaag ctgacgccga
    12181 tcaacgtcga cgacagggaa atcagcttcg gcacggccac cgatttcaac ctcgaggcca
    12241 gggagtcttc cgcacagcgg gccggcgact ccgtcgcctc ggcgaccggc gtggtcatca
    12301 accgcagcac cggcaagtac ttctcgatcg cgacgcgcaa gcagatcagc accgaaccgc
    12361 cgcccgaagg cagcaccgag ccggtcgtca ccaccaacgt cagaacccgc cagggcacct
    12421 gcaccgacgg cacgccctca gcttcgttct gaaggccgcc ccgcggggcg ccgggtcatg
    12481 ccaaccgcgt gaatcttggc tgccaccgac cgtcgaaatc acccgactcg aagaacatcg
    12541 cgtgcgggcg cacccagagg ccggatgcgt tgcctagcgg gcgatacacg accatcggtt
    12601 ccagcgtttc gctgtggcgg gcgacgccca cgacctcgta ctctcccccc ttgtaatggc
    12661 ggtagcggcc aagcggggtc tcgggcagcg caggcaattc actcatgggc gcagccggtt
    12721 cattcatctt gcgacagtga cagcacgtgg accacgccga acgcgccgat ggagacggtg
    12781 cgttcgttga cggtggtgta cgacgccacg ccgagcggat ggtaggtgga cgtgaacgcc
    12841 gtgaacacgc ccaccccgag agcaccggcc agcggactgc gctgcgcgat cgcttcccgg
    12901 atctgcgcgc gatgctgttc aggcgacggg ttgagcagga acgcagcggc agtgactgcc
    12961 gaagcgagca ctgcggagat caaggcggcc ttggccatgg tgcgtgggtc ttccaggaag
    13021 tgcagccagc tcgaggctgt ccgcttgagc agcgggtcgc acccggatcg gcgggatgcg
    13081 gcacccgttc gtgaaacccg attctgccgc gtcacgctgc cgtccagcgc accgtggcga
    13141 ctgcgtccgc atgggcagga ccaacgcgga tgtttcgcat ggccagggct gcgcgaagta
    13201 tactgtacat aaatacagtc atgaagggct gcgtcgcacg gcacggccca gggccgtttc
    13261 cgccccggca ttccacccgc ctgccgaacc cggtgcctgc cttgcccgcc tacgccgaac
    13321 tccactgccg cagcaacttc agcttcctca ccggtgcttc gcatccggag gaactggtcg
    13381 ctcgcgcagc gcatctgggc tacggggcac tggcgatcac cgacgagtgt tcgctgtcgg
    13441 gcgtggtgcg agcgcatgcc gaggcccggc gctgcgcgct gcatctgatc gtcggggccg
    13501 agatgtcggt cacgcgggca ccggccgagg tcggcggcgg ggcgcctctg cccgcgatgc
    13561 ggctggtgct gctggcgcag acacgagcgg gctacggaaa cctgtcgcag tggatcacgc
    13621 tcgcgcggcg gcgcgccggc aagggctgct acctcgcgca ccggttcgat ctggaagcct
    13681 cgagcgaagc ccatcgcgac aacgcactcg ccggcctgcc cggttgcctg gcgctgctgg
    13741 tgcccgatgt cacccagccg gtcgaacaga tgctcgccga cgcgcggtgg ctccattcgg
    13801 tgttcggcga ccgcgccacc ctcgcggtcg agctgctgct gcgcgcacgc gatgacgtgc
    13861 tgatcgatcg ggtgcgcgag gtgtcggcag ccaccggcct gcctgtggtc gcggcgggcg
    13921 acgtgctgat gcacgtgcgc tcgcgcaagc cgctgcagga cacgctgagc gccacccggc
    13981 tgggcctgcc gctcgcgcag tgcggctcgc agctcgaacc caacgccgag cagcacctgc
    14041 gtccgatcgg ccggctgcag gcgctgcacg agccggcgtg gctggcgcag acgatggcgc
    14101 tcgccgcccg ctgcaccttc tctctcgacg aactgcgcta cgagtatccg caggagatcg
    14161 tgccgcccgg caccacaccc gccggccacc tgcgcgcgct gaccgaatcg ggcgcgctga
    14221 agcggtttcc gcccgaggca tttccgcagg gcctgccgcc cacggtgcgc gcgcagatcg
    14281 agcacgagct cgcgctgatc gcgcagctgc agtacgagcc gtacttcctg accgtcgccg
    14341 acatcgtgca atgggcccgc tcgcagggca tcctgtgcca ggggcgcggc agcgcggcca
    14401 actcgaccgt ctgctactgc ctgcacgtga ccgaggtcga tccgacgcgc cagcacacgc
    14461 tgttcgagcg cttcatcagc gccgaacgca acgaaccgcc ggacatcgac atcgacttcg
    14521 agcaccagcg ccgcgaagag gtcatccagt acatctacgg caagtacggc cgcgaccgtg
    14581 cggcgctgac cgcggtggtc atcagctacc ggccgcgcag cgcactgcgc gacgtgggcc
    14641 gcgcgctcgg cctggatgca gaccgcatcc acgaggtcgc caggagccag cagtggttcg
    14701 acggccgccg catcgatccc gaacgcctgc gcgagaaggg ctacgacctg agctcgcacg
    14761 tcacgcggtt gtggatcgag ctgacggagc agctgatcgg cttcccccgc catctcagcc
    14821 agcacccggg cggcttcgtg atctcgcgcg gcccgctcgc gcagctggtt ccgatcgaga
    14881 acgccgcgat gcccgaccgc agcgtcatcc agtgggacaa ggacgacctc gatgcgctgg
    14941 ggttgctgaa ggtcgacatc ctggcgctgg gcatgctgac cgcgctgcgc cgctcgctcg
    15001 acctgatcag ccacaagctc ggccgccgct tcgcgctgca ggacgtgccg cctgaagacg
    15061 cagccaccta cgacatgatc tgccgcgccg acacggtggg cgtgttccag atcgagagcc
    15121 gtgcgcagat gagcatgctg ccgcgcctga agccgcgcgt cttctacgac ctggtgatcg
    15181 aggtcgccat cgtgcgcccc ggcccgatcc agggcggcat ggtgcacccg tacctgcggc
    15241 ggcgtgacgg catcgagcca gagacctatc ccaaccccga gatcgccgag gcgctgtcgc
    15301 gcacgaaggg cgtgccgatc ttccaggagc aggtgatgca gctcgccgtg ctggccgccg
    15361 acttcacccc cggcgaagct gacagcctgc gccgcgccat ggcagcctgg aagcgcaagg
    15421 gcggactcgg tcccttccac gagcggctgg tcggccgcat ggtcgagaag ggctaccagc
    15481 gcgagtacgc cgagaacatc ttcaggcaga tccagggctt cggcgaatac ggattccccg
    15541 aaagccacgc ggccagtttc gccttgctgg cctacgccag cagctggatc aagcggcacc
    15601 accccgatgc cttcctggcc gcactgctcg acagccagcc gatgggcttc tacgcgccgg
    15661 cgcagctggt gcgcgacgca cgcgagcatg gcgtcgacgt gcggccggtc gatgtgcgct
    15721 tcagccgatg gaacagcagc ctggaaccgc cggggcccga tgacgccgcc ctgcacccgg
    15781 tgcgcctggg cttcaaccgc atcggtggcc tgtcacaggc ggcggccgag cgcatcgttc
    15841 aggcacgcga ggccgacggc gcgttcatcc acctcgaaga cctgacgcgc cgcgccgcgc
    15901 tcgatgcgcg cgacctgaac ctgctggccg cctccgatgc gttgcgtgga ctggtcgctg
    15961 gttcgccgga accgcatgca tcggcacctt cgcggtcacc ccgcggccac cggcaccagg
    16021 ccgcctgggc ggtggccgcc atcgacaccc ggccgagcgc cctgctgcat ggcatgcgat
    16081 cccgcgagcc agccacagag gctttcccta tcacgctgcc ctcccccagc gcagccgagg
    16141 acatgctggc cgactaccgc agcacgggac tcacgctgaa ccgccatccg ctggcactgc
    16201 tgcgaccgca gctcgacgcc ttcaggatcg agaccgccgc ggtcttgcgc acctatccgg
    16261 atggtcgcat cgcacgcgcc agcggcctgg tcacgcaccg ccagcgtccc ggcaccgcca
    16321 agggcacagt gttcgtcacg ctggaagacg acaccggcac cgtcaacgtg atcgtctggc
    16381 cgacggtggc ccaggcacag cgccagccgc tgatcggcgc ccggctgctg acggtctacg
    16441 gccagtggca gcgcagcacc gatgtgccgg gcgagcatgc ggtgatgcac ctgctggcct
    16501 cgcgcctgat cgaccacacc gcgctgctga acggcctggt gacccgcagc cgcgatttcc
    16561 actgaccgcc agcgtgcgga cgccaaccgc agcatcgatt cacgaggatg gccttgaatt
    16621 ccagggcgat gacctaaact catcacctct gggaccgacc tggcttcgac gtgggtgcgg
    16681 atttggacta gggcatgccg agcaccagtt cgctcgtaaa accactggaa acaaagtaac
    16741 tgcgaacgac tctacgtacg ctctcgccgc ttaaaaccgg tgagcttcgc aacagttggc
    16801 cgatgggctg ggtctgaggg gtaacccaag gactgcgaag tcatacacat gggctcgtcc
    16861 tgcatcgcgt cattcgatgc aggatcaaac taagtgaatc gaggtgatct cagcgtgctg
    16921 ctgcgcggtg atcaccttta aatccaaatc agtcagctac gcatgtagaa ctacctgatg
    16981 atggcttgcg gacgggggtt cgattccccc cggtttccac caaacgatgg tccacatagg
    17041 gccataaaag acgaaacccc taagtaagtc aacgacttag gggttttttc atgcccgcat
    17101 gggaccggac aacaccactt acacccagct tatgaccgga taacataccg gataacaagc
    17161 cccaaccgga taaccggggc tgggagttat ccggtatggc gaccaacatc ctgagcgaca
    17221 agacgatccg cgcggcgctg aagaaggcga ccgaggcggg caagcccagc acgatcaacg
    17281 acggcgacgg gctgagcctg attgcccgcc cggacggcgc cggctggtgg cgcctgcgct
    17341 actggctgga cagccgggaa aaccggcttt cgctaggcac ctatccggac gtgtcgctgg
    17401 ccgacgcgcg aggtcggcgc gacgccgcac gcaagcagat cgcggccggc accgatccca
    17461 gcgaagcccg caaggcagac aaggccgcca agcagaagga gcgcgagtcg caagctctgg
    17521 cagatgccgg actgcccggc ccggggacgt tcgagcacgt tgcccgcgaa tggctgacga
    17581 cggtccatga ggccaaggtg agcgccggac atgccgagcg cacccgcatc cgacttgagc
    17641 aggacgcctt cccctggatt ggccggcgtc cgatcgcaga catcgaggcc cccgccctgc
    17701 tggaatgcct gcgccgtgta gaggcacgtg gcgcgatcga gacggcccac cgcgtcaagg
    17761 atgcctgcgg gcaggtgttc cggtacggca tcgccatcgg tcagtgcaag cgcaaccctg
    17821 cgtccgagtt acgcgacgca atgcggcccg tcgtgagccg gcaccatgcc gcgatcgttg
    17881 accctgttga ggctggaaag ctgttgagcg acatgctggc ctacgaagga cacccggtga
    17941 cccgtgctgc gctgcaactt tcggcgctcc tgatgttgcg accgggcgaa ctgcggcaca
    18001 tggaatgggc ctggatcgac tttgaggaaa gtaccttgac gatgcccgcc gaagtgatga
    18061 agcgcaaaaa ggccgacaag gccgatggtc cgcctcatct ggtgccgctt gctcagcaag
    18121 ccgtggcaat cctgaaggat ctgcagccgc ttacgggtgg cgagcggcgt cgcttcgtgt
    18181 tcccgtcact tctcaccggc gaacgctgta tgagtgaaaa cacggtgcgt ggcgcactgc
    18241 gccgaatggg ctacgccaac gacgagatga cggcccacgg ttttcgcgcc atggctcgaa
    18301 ccatgattgc cgagcgcctc gaaattgccc ccgaagtgat cgaggcccaa ctggcacacg
    18361 ctgttggtga ctcacttggg cggtcgtaca accgcactca gttcctcgac cagcgccgcg
    18421 agatgatgag caagtgggcc gattacctcg accgcattcg cataggggcg gaagtgctgc
    18481 cgatcaaggc cgcatgagcg ttccgtgcca gcaggaaacg ctgcggtggg ccctgcgccc
    18541 gcactgcgat aacgaagact cgcgcaacgc ctgggtaacc gacttccgcg tagagctcgg
    18601 ccaggtgtca gctctcgacc ctaagcagcc ggttgatctt tcctgaagct gcccgacggc
    18661 caagcgccaa ctcccgggtt tcgtaccctc gaagcagccg ctcgcggtca ccggcagcag
    18721 gtcagccgta gacaatcgca gcatggacaa ggcactcacg gaagaggacc gcacgactgc
    18781 gattgggctg gctcggtacg cctacgacta catcggagcg gcgatgctgg tcgagcgtaa
    18841 cgaccccatg ccaactcatg tatcaccggt ccccgcgtac ttcctggcgc tgcacggcat
    18901 cgagctgacg ctgaaatcct acctgcgtca caagggagtg acggcgaagg aactgcgcgg
    18961 ccagaagtac gggcacgacc tgcacgcctg ccacaagaag gcaaaggaac tcggcctgct
    19021 gtccatcttc aaggagcagg ccaacgacgc cgacgcgatg ctcatgcttg tcggtttgaa
    19081 cgagcatcag ggtctgcggt acatcaagac tggcatgaag cgcttcccgc tctggtcgtt
    19141 agttgagccg ctttcggtgc ggctgcatca agccgtagct ccccaggtcg gatacaagtc
    19201 cttcgacatc acatatcacc aatgaaccca gccgcacgcc tgcaccgcat ctacgacaag
    19261 ctggtcagtc agggcaacga ctatcagttg ctccagacct gggccgacgt tttcgacatc
    19321 gacaagagcg gcccgcacta cgaggacgac gtaaccgcat gcgtgatggc actccggtcc
    19381 gagattgccc tgactcgcaa gcggctagac gaaatcggtg cccccgcgaa cctaacgagc
    19441 ccaggctttg agcggctgcg tgaagtggca gcgccgggtt tgctgcatgg gaagtggcac
    19501 agccatcgag gcaacatcag ccctcccgag tgtcggaagg tgttcgagtg ggccgaatgg
    19561 gccctgcgcg aagacgccga gccggacatg cccgccgagg acatgaacga actgcgggcc
    19621 gaactggaat cgcttgaggc tgcactcaac gacgcggagg tgtcaccata cctgcgcgac
    19681 ttcatccagc ggcaggtgga caccatacgc gccgcgctac gggtgtacgg ggtgcagggc
    19741 gcacgaacat tgcaggacgc attgcaaaag gtggccggcg cctacacggt cgaacgcgct
    19801 cgggttgagg ctgagcatgg cacagcaccc gaggccacga agggtctgtt cgccaaggct
    19861 agcggcgtca tcaagaagac ggcggacgtg tgcgacaacc tgggcaagat aggcaagttc
    19921 ggtgagcagg cttggacact agctggcacc gttgggccgc tggtgctgcc gtacctcccc
    19981 aagctcggcg ggtaagccga ccgcgcgtcg ggatagacgg ggccaaagta gagcgcaaac
    20041 tggtcgccga aagcaagtca tccaatgctg gccatcagga gttagtctga actgatcacc
    20101 aggggccgct catggccgtc agcgtgagtt agccagggag gatcttgtcc tctaggcgcc
    20161 agagttctgg cacttcgagc acatcgcaca gccctccaat ccggatcccc cccctggact
    20221 ggcgggctgg agatgcccac agtcgcacca ccgtcggcgc catttccgca tatatgcggc
    20281 tgttgcaacc aagctgttgc gaatcacgat gtgcggcatg acaaaccgca ccacaccgcc
    20341 tagcaccgca gccacccgca ctgcagggaa cgtcgtaccg atcgctcgtc cgacggtccg
    20401 gccgccattg ccgccggctg atgaccccca cgcactgatc gacattcgtg gcgtccagac
    20461 gctcacttgc atgtcggaat cgtggcttca cgacgccatt cggcgtggcg aattttgccc
    20521 gcctctgcgc ttaggtccac gctgctcacg ctggcgaatc gctgacgtac gcgcctggct
    20581 gatcaagcaa ttcgaggcgg cctcggcaga cacctcgacg gctgatctgg tgagagcgcg
    20641 agcaaagaag ggagccgacg ctgccgcccg gcatcgagcc gcgaagaagc accgtcccga
    20701 ttgcactgac ggcggtgggt cgtcatgagt cttcactcct gtttcgtcag tctctggatg
    20761 gctttccatc ttctgcgcca caccaattgg tgcgaaggcg caaccagcta tctcctctgc
    20821 gggtgcatcg cggcgacctg cgagctacct gacctcgacg ctgtaactca cgtctcttag
    20881 aaaccaggta agccccatga ctgattcctt cggcagcttc cgcacagagc tcttggccac
    20941 actgggcggt gcgcccgcga cgattgagcc cggccgtctt catcgattca gcaccagcga
    21001 gcggcgtgga gattcggctg gctggtgcaa gctgttcgat gatctgcgcg gtggcgttta
    21061 cggatgccac cgtcagtttc ctggcgagtc ttttacttgg agcgcgatcg accggcgcac
    21121 gatgacgctt gaacagcgca gtctgctggc gcgacagttc gaggctgcaa ccgccgaacg
    21181 ggaagctcag cagcgcatgc aatggtccga aaacgcccag cgcatcgctc agatgtgggc
    21241 ccagtgcgtc ccgatttcac cagacgaccc ctgttctgcg tatctcacgc agcgtgggtt
    21301 agtaggcgcg tggcccctgc ctgaggtgct gcgattccat ccctcacttc cgtactggaa
    21361 cggggtcgag aagctgggac cctttcctgc gatggtggcg ccgctcatcg ctcccgacgg
    21421 ccgtaccgtg gctctgcatc gcacttatct cacagcacac ggacgcaagg ccggcgtacc
    21481 caccccgaag aaggtcacag gtgcttcggg tgtgctggct ggcgcaagca ttccgctgca
    21541 cgagccgacg ggcggcgtta ttggcatcgc cgaaggcatc gaaacggcgc tggctgcgtg
    21601 gtgcatatcg ggtgtgccta cggtggcggc gtactgcgcc ggcaacctgg ccgcgtggca
    21661 gtgcccggcc ggtgtgcagc gcctggtggt cttcgccgat gctgacaagg caggccgtga
    21721 ggccgcagac acgctgcgtg ccaaggcact gtcgcggcac ctacgctgcg aggtcatgac
    21781 tcccacggac gaagggtccg actggtgcga cgtgtgggcg gcgagccgac cgacatcgat
    21841 caacgcggag gtggcagcgt gagcgccgtc gtgcaagacc tgacgcgcgt cgccaggctc
    21901 gccgaatcac tcgcggtgga tcgagctgaa gccatcaagc gcgcccgcac agcactggcg
    21961 ccgtctctcg ccggccgaca ggcccctgcc tatcccctgg cagcactcgg gccgctggcg
    22021 ttcgcctgcg aggccatcgc aggagcggga caggtgcagc ccgcgatggt cgggcagtgc
    22081 ctgctcgggg ccgcatcgct gttgacgcaa gggcttttca acgtagagac attgaacgga
    22141 aagcgaccgc tgtcgctgta ccttctgacg ctgggggact ccggcgaggg caagtccacc
    22201 gcgcagggtg cggccctggc gcctgtcaac gactggcagt ccaaggctgc gaaacactac
    22261 ggcgaggcgt tgaaggactt cgacaaagcc aagtcgagcc gcaagcgcgc tgacgatccg
    22321 cctgagccgc cagcctgccc gtaccgcctc gtcggagatg ccaccgtgga aggactgcgc
    22381 cgagacctcg atacaggcgt ctgctcgcaa ggcgtgttca gcgatgaagc ggcggcgatc
    22441 ctttccggct acggcatgag ctccgaccac cgatcgaaga cggcgggggt gttctcgaag
    22501 ctgtgggaca acgggcacct gagcgtgtca cgtgtgactg gcggacgagt cgagcggtac
    22561 ggacggcgtg tggcgctgca ctggttgatt cagcccacgg cggcatccga gtcaatcggc
    22621 gatccgctgc tatcggcact gggcttttgg ccgcgcttcc ttgcggcatg gcccgatgcg
    22681 caggcacctc gcctggcacg ctctttccgg ccggacacgt tgcctgctgt cggcgcctac
    22741 tggcagcgct gcaacgggtt gctgaatgag gcgctgcccg acgatgcggg agactgcccc
    22801 gtgctcccgc taaccgaaga tgcccgggcg catttgagct cggcgttcga gagattcgag
    22861 cgtgatgccc ggcgaggcga gctgcaagtc gtcaagccgt tcgccttgcg cgccagcgag
    22921 caagcctgcc gtatcgccgg agtgctgagc gcgttcgagg gtgcatcgtc ggtgtctgcc
    22981 gacgcgatgc acggagcgct ggcgctggtt gcctacagcc tgaaaacatg gcagaccatc
    23041 gtcgacgagg gagcagccga tcagcgcggt gcgatcgcgt tgcgactgta cgaatggctg
    23101 accaaccggc cgggttggtg cgagccactg gcggtgatcg tgaataaggg gccggcctgc
    23161 gtgcgcacca gagaccgacg cgacgcggcg ctggcggagt tggaggaggc cggacttgtg
    23221 caagtcatcg aaggcaaggc gcaggcgctg atcggccaag cgatcgatgg ggcaacgcca
    23281 tgacgtccgc gctcttggat gaactgctgg ccaagtttgc tagcgggact gtcttggcga
    23341 ttgcaccggc cgcccgcgcg aaagctgcga attctgcgaa gagtgagcat ccacgtgggc
    23401 aggaccctgc attgcgagtc tgcgaaggct tgcgaacttc tgcgaatgcg gtcggcatcg
    23461 ggcgtcgaga cgatgcagat tcgcagaatt tcgcagacat tcgcaaacca gaaagaggac
    23521 cgcacatcga ggagtcgtgt ggatcttcgc aggattcgca ggattcgcag gggcaccccg
    23581 tgcaatgcat cgcggacatc gctcgcgatt cgacggcgat cggctggacc gatgccgaag
    23641 tcgcccgttt cctcgaccgg cgcgcacgcc tcatgcgctg gggctggcca gagcttgagg
    23701 cggaagccct ggccgatcga ctgttgcgcc gcgaccaaga tcacgacgac cgggtgagct
    23761 gcgctgactg caaacactac cggcctggcc gctgtggcaa ccacaggcgg gctggcctga
    23821 acgtggccga cattggtcgg gacctggcga ccctactcca acggtgccca ggatttgacc
    23881 caattggatg acgacatgac gcgcaagaac cctgattcga ccctgatagc ttcggatcga
    23941 ctgacgtcca aagaggagaa cttcgcgcgc cttcgtgccg cgggcactcc gcagggtgac
    24001 gcctatcgcc aagcttatga gtgtgacggc agcaccacca gaaccgttgc agttgagtcc
    24061 agccgggtgg agagcaagcc gagggtgaag cggcgaatcc tcgaactacg agatgcccat
    24121 gccagtgctg tcgtgagcta cagcgttgcc gctgcaatga gcgaactgga cgaaagcatg
    24181 gcgctggcga aggcgaagga aagcccgatg gcgatgatca aagtcgttga ggtcaggatg
    24241 aagttgtacg gtttgggcat cggagaagcc aagaaccagg ccgacaagga cgagatgacc
    24301 cccgaggagt tggaggctgc actcgcggac gttcgtgcac tgagggctgc ccaggccgct
    24361 tcacatcact agcgtggtgc gctcgagctc attgaatcca tcggtgcagg gcaatgacgg
    24421 cttcaccgct ggcggacctc acactgtccg ccagctcttg accttcaagt cgcgcgagcg
    24481 aacggcagga acagactgac tgcggccatt cgccctcgcg cgcgccgctg tcggcttccc
    24541 gccccgaaga gacattgcag acccgactgg gtgccacacc gccgcctgtc gcgcggcggg
    24601 cgccttgacg atcacatcaa tccggtcgtc ttgagagctt cgagcaagct gttgccaatc
    24661 gacgtgatcg actccatggc tggcagcgac gccagcagcg cggccaagat cgcatgtttg
    24721 ggttttccag acgtaatctg tgcctcgacc tcatcgacca cttccactgc tgagccacgc
    24781 tgtacctcgg agagcggcgc ccgttgaatc tcgtctcgta gctcccggat gcagttcatc
    24841 acagtgctgt taatcgtcac cgcgttgacc gagttgtcga tcgactgatg gttcactcga
    24901 gcgttgtttc cgttgatgtg gtacgtgata tgcgcagggg catttccgac gccatccgca
    24961 acgccgctca aggacgccgc tacttccgat cgatctatgg tcatcacata gtccttgtag
    25021 tcgcgagcga acgggatcag caactgacgt gtgacgctgt gcagctcacc ggtgaggttg
    25081 cgcccaaccg tgtagtacgt gtgcgcgaaa ttcaacaagt ctcgcggttc acgcgcgaac
    25141 ttttgaacca acagccactg caggccgagt acctcatccg cgttttcagg ccaggcgagg
    25201 cgtgccgaac ccaccatgct gccttgcgac ctagaactgg cttccaggaa gcgctctata
    25261 tctagattgg ccgttagctt ctgattcagg agttgcagtg atgagtccgc aaaaagtcgg
    25321 gcaaaggtaa gaaagtgctg ctcgaaggtt tgcgacgttg ccgcctgcat gtcgaacaag
    25381 gcattgttaa tccgcttaaa cagtacctcc ctattcgcca tctgacgcct ctcccgtcag
    25441 gcgggcctca gctgccttct cgatcacggc agtcctgaac gagtccttca tctgaactat
    25501 cagcgggccg acgatcgagt tgttggcgta cttgcccagt cgctcgtcga ccaaccgctc
    25561 ggactcagcg atcgccgcct cgacgctccc tggatcctct ggattgaacg acaaggttcc
    25621 gagctcgccg ttgacggcct caagcgctat ctgcgcctcc gccaaagttc tctgcagatc
    25681 gtccaggcct ttcagaaatg ccatagcaac tcctccctat tcgtcggtct gtatgggcgc
    25741 aagtgggatg cgaaaccgcg aatacgccgt ctggtgcgct gtctattcca tcatgaaacg
    25801 taggcacagc gaagcccaag gaccgcttgg tgcgagctag tgtgagctcg gctcgctgag
    25861 gcgatggact gcaatcgctg cagagttgtc agtcgcgaat cgattgtcgg ttcaagactg
    25921 atctgagaca gtcgcggccg tggccaggac gactgctttg acgaacaccg ttcatggacg
    25981 gtcggcgacc ttgtccggca gcttcgcccc aaacaccggt cgctcgacgg cagtggacca
    26041 ccagggcagc acgaacggcg gctcccacct accgtggtgg tctcaggtcg accagttgcg
    26101 gtcaaacgcc ggccggccac caccgaccgc tttgtccagc aaagctgccg gactcactgc
    26161 tgaagccagt cgagctctcg ttgcatctag gcagttttca ccgatctctg gcggccttcc
    26221 gactggcatc atcttgtcat catcgggcca agctccgagt ggcgagaact tgcaactgca
    26281 cgccgttctt gtcagatatt taacgcccgt ttcgcctgtc aatttttcga ggaagtccaa
    26341 tgagtcaagc acttgcgacg acacaacagg gatgggttgt gcggcagcat gcctgcctga
    26401 gcagcattgg cctcctcccg agagcacgtt agagcgcatg agcacgcacc cgatgacacc
    26461 cgagcgtttc gagcaattcg cccgacaacc ggctcgcacg cgtgaagagc ttgagactat
    26521 gcgcgcgaac gcactggcga agggcaatgt cgagttggcg cacatcgcag ctgaagtctt
    26581 ggctgaacgg tttcccaccc aaaccaagca gcggtcgggg ccgacgcaaa ccacagcagc
    26641 tttcctcggc cgctcagaaa cgttcggcac gggcaaggag gcgtatctct ggctcctgga
    26701 gcgatttagg cagcatcaac cgttgatttt cgaggcatat gaactgcttc gacacaaaga
    26761 tcggtcggca ggcacgcgcg tcgcgaagga cccagccctt ctctttccaa ggggttcttc
    26821 ccgcatcggc aatccagcca actacgccac cctcacaggc gggtggtatg ccgatacgaa
    26881 tctcaatcat caagacaagt tttcaactct cgttcggttc gctcaccttg ccaaacttga
    26941 gtacccggaa gattgggact tccgtgtcga gggaggcacc atggagctcc gcaagcaaca
    27001 agatctcgtc attcaagtgc gtgaactact acgagagctg ggcctcgcga attgagctct
    27061 aaccattctt tcgagcggac gcctaacggc gccgatcatg gataaattct tgctgcagca
    27121 gcaggtgctg gaacggctcg ccgaagacct gctgcaagcc gaacaggcag tgcgggcggc
    27181 ccatgaaacg gcgacccacg aagagaacat cgccgaaaac aagtacgaca ccttggggct
    27241 cgaagccgcc tacctggcca ccggccaggc tcgtcgcgcc gaagccatcc gccaggcgat
    27301 ggcccattgg cgcgaattcc gaccgagccc ctacgacgcc agcaaaggta tccagctcgg
    27361 cgcgctggtc tgcttggtcg attccgacga caagcagcaa cagctctttc tcggcccgga
    27421 tgggggcagc atgaaattgg tcagcggcgc tcaggtcgtt caggtggtca gcagcgaagc
    27481 tcctttgggc agggccatgc tgggtaaatt cgagggtgat gaggtgtcga tacaggtcgc
    27541 tccaatccga cagcagtttg aggtgctgcg ggttcattga gccgcaagga tgaccgctgt
    27601 gaggcccaaa ccgtgaattc acgggggctg catcaccggc agcaaccgct gcattgccgt
    27661 cacgcagcgc cggtagatga aggtcggatc agggtcgtgc agagaccttc gacttgccgc
    27721 ccgactgcag acactcggtt tcgccgatct ctgcgacacg cgatcgctcc atcccgggca
    27781 aggttcccgg tctcgcaggt gacaactgcg cgtggtcgat caacaagtac agaacggagc
    27841 ggtgcactgc aatagacggg cgcgccccgc ctgaaaggat cgccggggga cggcaccgaa
    27901 gctcctgact gaccatgcgt gggcagattt gaggtgcggc agttcacccc gtgaaccggg
    27961 ggtgctcgcc tgggtcatgc acgcttaatc tggaggtcaa cccaaccgcc agtcaaagga
    28021 attcccatgt ccatccgttc aaagctcatt gttgctgccg tcgccacgtc ggccatggtt
    28081 gtttcggctg cggcccatgc cggagctttc gtccccccta atgtcgtgcc tgagccagga
    28141 accttggccc tcgtgggtct ggccgcggtc gtcggaatcg ttgttgctcg gaatcggcgc
    28201 aaataaccag cccgttcccc tttatgtact gcagttgact catggagggg ctcgcccgcc
    28261 atgagtcgct gatccagacg ctggccctgg tgcttgcgta cctggcagtc gtgctttggg
    28321 agcgactgcg accattgctt cgttacgacg ccgacgaccg ctggcgtctt gccggtaact
    28381 tcgggttgct ggtcgtcaat cacgcagtgc cattcctggt ggttcctgtc gtggcagctc
    28441 tcggttcctg ggccgcctcg ggcctgaact tgggatggct gaacgcggtc cacgcacccc
    28501 gctggctgat gttcatggtg gccgtcgtcg cgcttgatct gacgggctgg ttggtgcatt
    28561 ggtcgatgca caaggtgggt ccgctttggc gggtgcatcg cgtgcaccac agcgaccttc
    28621 gg
//
LOCUS       DAOORV010000033        28550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1916_length_28550_cov_7.151676, whole genome shotgun sequence.
ACCESSION   DAOORV010000033 DAOORV010000000
VERSION     DAOORV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28550
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_28550_cov_7.151676"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..511)
                     /locus_tag="PKV56_06505"
                     /pseudo
     CDS             complement(8..511)
                     /locus_tag="PKV56_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(508..1254)
                     /locus_tag="PKV56_06510"
     CDS             complement(508..1254)
                     /locus_tag="PKV56_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOX67500.1"
                     /translation="MASNWDWQVFCKSTLEGEIQPSCFGTGGDITYLDWLLSAWGWTL
                     SVALLALVVALSVGALMGILRTAPSRTLVFIGNAWTELFRNIPLLVQVFLWYYVIPEL
                     IPPLKQVPSFLLVVFALGFFTSARVAEQVKAGIQALPKGQRYAGLAMGLTLPQTYRYV
                     LLPMAFRIVIPPLTSESMNIIKNSSVAFAISISELTMFALQAGEETSRNIEIYLAVTG
                     LYFLSAFIINRIALFIENRVQVPGMIGGGR"
     gene            complement(1307..2197)
                     /locus_tag="PKV56_06515"
     CDS             complement(1307..2197)
                     /locus_tag="PKV56_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX67501.1"
                     /translation="MKKTAFAFAALLACGVAQADTLSKIKESAAVTMGVRESSGALSY
                     TLGDGKYVGYHIEICERVVKAIQKQLGITAMTTKYQPVTSQNRIPLVQNGTVDIECGS
                     TTNNATRQKDVSFAVTTYVEEVRIATKTSSGITGIAQLNGKKVATTTGTTSVQTLRKN
                     ERAGGINFDEVYGKDHADSFLLMESGRADAFVMDGQILAGLISKSKSPADFKIVGEVL
                     SVEPIAIMFRKDDPEFKKVVDATIVGMMQSGELAKIYDKWFLQPIPPSNTRVGLPLSD
                     ATKAAWASPNDKPMEDYAKK"
     gene            complement(2362..4248)
                     /locus_tag="PKV56_06520"
     CDS             complement(2362..4248)
                     /locus_tag="PKV56_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOX67502.1"
                     /translation="MRPATLTPPAPADAAAGPARSDWGTLKKLLPYVWQWRHRVALAL
                     TCLIAAKVANVGVPLLLKNLVDALALKPGDPQAVLVVPVALLIGYGALRLSITLFTEL
                     REFLFYPVAARIARRVGLETFDHLLSLSLRFHLERQTGGVSRDIDRGARSIQSLLNYL
                     IYNIVPTLVEITLVISLLSAKFDGWFAAITFSALLLYIVFTVTVTEWRTAFRRAMNEQ
                     DSKASTKAVDALINYETVKYFSNEKFEQARYDENLQRLERASIKSQTSLSVLNLGQSL
                     IIAVAVTLLVWRATEGVVAGTMTLGDLVLVNALMIQLYIPLNFLGVIYREIKQSMIDM
                     EKMFALLGQNREVADAPDARPLAVDQAAAGAEVRFRHVRFGYDADRTILHDISFEIPA
                     GKTVAVVGPSGSGKSTLARLMFRFYDVSAGCIEIAGQDIRTVTQQSLRQAIGIVPQDT
                     VLFNDTIEYNIAYGRTSARRDEVEAAAQAAHIHTFIASTPKGYDTMVGERGLKLSGGE
                     KQRVAIARTLLKNPPILIFDEATSALDSANERAIQAELQGVSRNKTTLVIAHRLSTVV
                     DAHQILVMEQGRILERGSHAELIAMNGRYAEMWRLQQSRGDGLQGAGDADEAVGPDEQ
                     AVPA"
     gene            4439..4783
                     /locus_tag="PKV56_06525"
     CDS             4439..4783
                     /locus_tag="PKV56_06525"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOX67503.1"
                     /translation="MPMPADTNMSGGVFGGWIMAQVDIAGAVLPARVSRGRVTTVAVN
                     RFIFKQPVSVGDLLSFYARVDRIGTTSITVHVEVYAERNPSNPYIVKVTDANLTYVAI
                     DGQGRPRPVPRD"
     gene            complement(4806..5570)
                     /locus_tag="PKV56_06530"
     CDS             complement(4806..5570)
                     /locus_tag="PKV56_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOX67504.1"
                     /translation="MNIKTATLNGVATIEIARPQKKNALTAEMYSAMADALRAADAAA
                     AVRAVLIVGQPGVFTSGNDIDDFMNRPPATSDAPVFVFMRALVACSKPVVAAVTGAAI
                     GIGTTMLLHCDLVYVSDEARLAMPFASLGLVPEFASSLIVPALVGRARAAEKLLFGDP
                     FSAEEAVEMRIANAVLPAGEVARHARRMAERFNSLPPGAVRESKQLMRRASEAAVLEA
                     IEIEAAAFRQRLSSPEATEAFSAFFQKRKPDFSTFS"
     gene            complement(5590..6789)
                     /locus_tag="PKV56_06535"
     CDS             complement(5590..6789)
                     /locus_tag="PKV56_06535"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOX67505.1"
                     /translation="MSKILQDAYIVAATRTPIGKSGRGMFRNTRPDDLLVAAIRGALA
                     QVPTLDPKAIEDAIVGCAMPEGEQGLNVARIGALLAGLPNSVGGVTVNRFCASGLTAI
                     QMAADRIRVGEAEVMIAGGVESMSMVPMSGNKPSFNPAVFLRDENVGIAYGMGMTAEK
                     VAQQWKVTREAQDEFALQSHLKALKAIEAGEFSNEITPVDVTDRSPDLQTGEVVSKAR
                     TAAVDEGPRADTTLEALARLRPVFAAKGSVTAGNSSQTSDGAGCLILASAQAIKTFNL
                     QPLARFVSFANRGVPPEIMGIGPIEAVPAALRYAGLQQKDIDWIELNEAFAAQSLAVL
                     GALHLDPARVNPLGGAIALGHPLGATGAIRAATVVHALRRHNLKYGMVTMCVGTGQGA
                     AGILERC"
     gene            complement(6927..9335)
                     /locus_tag="PKV56_06540"
     CDS             complement(6927..9335)
                     /locus_tag="PKV56_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOX67506.1"
                     /translation="MKRFPVRKVAVLGAGVMGAQIAAHLANVRVPVVLFDLPAREGAK
                     NGVVTKAVDGLKKLKPAPLGVNDDAVLIQQANYEEHLDQLRDCDLIIEAIAERMDWKL
                     DLYTKIAPFVAPHAIVASNTSGLSISKLGAVLPEAIQPRFCGIHFFNPPRYMALVELI
                     PTPATLPDILDQLEAFVTTALGKGVVRAKDTPNFIANRVGVAGMLAVMKEAEQFGLSC
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTLAHVIRTLQDNLGPDQSNDPFYGSYATPP
                     VLGALIAKGTLGQKTGAGFYKKVGKDILRWDAAANDYVTGGGQADELIGRILKKPPAE
                     RLKLLRESNHPQAQFLWAVLRDGFHYSAVHLAEVAECARDIDFAMRWGFGFSQGPFEL
                     WQAAGWSTVARWMQEDIDAGRALCPAPLPAWVFKGPVADREGVHQPEGSWSASESRFV
                     AESALPVYARQHFRERVLGSGAPVPLQSGTELFKTDELRVWTLDGEVLIASLTAKLHL
                     ISPAGTEGLVRAVALAEESYKGLVIWSPDDVFSAGANLESLMPVFMKSGAKGIAPEEQ
                     KLQDAMLRVRYATVPVVAAIRGLALGGGCELAVHCAKRVAAMESYVGLVEVGVGLIPG
                     AGGLTYIARRAAEMAGAGGANADLLAFLKEGFAAAAMAKVGTSAIDSRQLGYLLWSDV
                     IVPHKDELLFVALTQARAMFDSGYRPPAKTLFPVAGRSAAATIKGQLVNMRDGGFISA
                     HDFHIASLIAETLCGGDVDAGSLVSEEYLMALERQHFCSLLEQPKTQERIMGMLSTGK
                     PVRN"
     gene            complement(9409..11214)
                     /locus_tag="PKV56_06545"
     CDS             complement(9409..11214)
                     /locus_tag="PKV56_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOX67507.1"
                     /translation="MALYTPPLRDMRFVLHELLKVTDTFKLLPPHAEVDIETIDAVLE
                     EGGKFASEVLAPLNQTGDAEGCTLNRDTHEVATPAGFKDAYQQFVEGGWPSLGCDAAH
                     GGQGLPVALNQCLYEMLNSANQAWTMYPGLTHGAYECLHAHGTPEQQQTYLPRLTSGE
                     WTGTMCLTEAHCGTDLGLLRTKAIPFEADGQADGSFLLTGNKIFISAGEHDLAANIVH
                     LVLARLPDSPPGSKGISLFVVPKFLVNADGSLGARNPIFCGALEHKMGIHGNATCQMN
                     LDGARGWLVGPPHKGLNAMFVMMNAARLGVGMQSLGLTEVAYQNAAAYAKDRIQMRSL
                     TGAKAPDKPADPIIVHPDVRKMLLTARAYAEGGRALAVFTALLIDQELSSSDESVKQD
                     AADLVALLTPVVKAFLTDNAWIATSHCMQVFGGHGYVREWGMEQFVRDARINMIYEGT
                     NTIQSLDLLGRKVLGDHGRKLKKFGVLVQQLIEDEGVNEAMSEFINPLADLGDKVTKL
                     STELGMKAFGNADEVGAAAVDYLRIVGHLVFAYFWARMAKVALDHADNGDPFYRAKLA
                     TARFYFAKLLPETAALIRTARAGAKPLMDMDESLF"
     gene            11434..11991
                     /locus_tag="PKV56_06550"
     CDS             11434..11991
                     /locus_tag="PKV56_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOX67508.1"
                     /translation="MEVLQLDVSGRPQSWLSAKEAAVLYASDGVAWTLGDAFFVLRGG
                     VQRRTGVQSRIEVHPIIAVRGAIPSRAWRQSPALSNPKLFVRDRHVCAYCGGHFHSDD
                     LTREHIVPTSRGGQDTWMNCITACRSCNGNKGSRLPEEARMSLLYLPYVPSLHEDMIL
                     RGRRILTDQMEFLLASVPRHSRLRT"
     gene            12137..12628
                     /locus_tag="PKV56_06555"
     CDS             12137..12628
                     /locus_tag="PKV56_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOX67509.1"
                     /translation="MKKAIALLALLGTLAGCSTTSPDVIQRGDAQRLSQVQDATVLSV
                     RAVTVDGSQSGAGAATGAVVGGVAGSTVGGNKTGAVVGVLGAVAGAVVGNTVERFATK
                     EDAVEVLIQMRNGDRRAIVQAKGNETLAAGDAVVLVTTGGKTRVIKAPAITSPPPPVE
                     PKS"
     gene            complement(12641..14353)
                     /locus_tag="PKV56_06560"
     CDS             complement(12641..14353)
                     /locus_tag="PKV56_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX67510.1"
                     /translation="MKNSLTPGRQPSAADVQRLVGLLNAGQWAQAEAEVRKALAAHPQ
                     ALVFLNLLGSALAGQSRWAEAIESFSRVAAVAPQSMETHSQLGMLYSQLGRREDAVAS
                     YRKAVALKPDFAELHCNLGILLGQLGRHAEAADSYAQAVAVKPALAVAHYNRGTALQA
                     LGRLDEAAQSYRRVLALQPASAEAHSNLGAVLQAQGALDAAIDSYRSALAIQPDPRVH
                     FNLGTAYRNEGRLAESVRSFESALALAPVYPDALNNLGEVLRDQGRLDEAVACFEQAL
                     AQDPDTSEANYNLAVLLYDRGRPDQAARYFEASRLRDWQQRVLLCLYKTEQFNAFRAG
                     LERLLAAPEAHAAPLLATLSAHHAANFGVTDHCDFCPHPLDFVSHGRIDALAGERSAL
                     REQLLIDIASAEISARKQGRLHHGIQSSGNLFKRPEASFRALSGLIAEQVERYRQGLA
                     GAEGVFARDFPATTEFSSSWYVKMQTGGHLTSHIHETGWLSGAVYLAMPPRDAGSDAG
                     SIEFSTDGDGYPRRHVDFPRRVCPPAVGDIVLFPSSLFHRTVPFSATEERICIAFDIA
                     PRMA"
     gene            complement(14380..16044)
                     /locus_tag="PKV56_06565"
     CDS             complement(14380..16044)
                     /locus_tag="PKV56_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67511.1"
                     /translation="MDPASECDREGGSAAGLIERTLSDLGPRVSPNGRFELGYTLQFP
                     LLRLLREQGSDWVVDQPAVERLVRTLHDVDRPAIVYLFSTHFGVDSPIEKVLMQDPAN
                     LAVTPVGSMTQDRYYASDVYPWTFSSTENGITRRRVQVIDAVVDEICRLPQHDRDKIK
                     GVTLLGELHHLFPNFQAGMGFRERYLVSDHSAASISGFRAFLAARFGSVAALNQAVGA
                     DYPSFDAVDPPSRDIRSQPLTRFTEHIDSFAHGTLPISGWVHLMAPGAREQLWVRIYR
                     DGEFVGRAAVGLSRQDVFEARPEFGTADVGWRFDLDHAGLAPGVHRIDLILEGHGDNG
                     VLIGTRHVSVMDRNQTTPRDVPAKALPITVPADASVLSYVDLPAERSSYYYNPLVPLW
                     HEFRGSQVVDYLRYFDARVRRSCLSEVDTYIHQITPFTNPGWDVNKFAVDASLAPFGR
                     AHLGVSLYGEPTYGTSFFDWYARSKHKTYGLTEFHPLRAMAPDEARRMFDHHRTSGAQ
                     FLSFFLDTRTAAGSAPVLRNQFSFDPANPSFGSDRLYDTVKTLLGE"
     gene            16043..16258
                     /locus_tag="PKV56_06570"
     CDS             16043..16258
                     /locus_tag="PKV56_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67512.1"
                     /translation="MAAGAARDGAPRQGSITFIMGAISSLEGGAGLCHATQAPNATAV
                     KATAGAATRQNLRVIIVGLDRSASRQC"
     gene            16437..17486
                     /locus_tag="PKV56_06575"
     CDS             16437..17486
                     /locus_tag="PKV56_06575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX67513.1"
                     /translation="MRLAELSSGKDNNFNLIRLLAAIAVLVSHSMVLATGHFRDNPFP
                     VRLQTSFGSIAVDVFFIISGFLVTGSLTTRQDLRQYLLSRVLRIYPALLVMVALTVGV
                     LGSYFTTLPWRSYLADAETWRFALMNATLVFGHADFLPGVFDATPMRGLVNGSLWTIP
                     HELRCYLLLGGVWLLASAARSSCMRVFRMACIAVALAFWIAHPVDHFYLKTGWPFIQF
                     FFMFMSGSIYFLLREHIHLSSRLFLVMVAALIASTFQNDAFFLVYRFTLPYIVLWLAF
                     VPSGSIRCFNKAGDYSYGFYIYAFPLQQSIISLMPQTSTLQLILISGSVALFFSVFSW
                     HTIEKKALQFKAAPK"
     gene            17560..18564
                     /locus_tag="PKV56_06580"
     CDS             17560..18564
                     /locus_tag="PKV56_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOX67514.1"
                     /translation="MSNNEPGGLWIDRPDALQILDQKKKSGIVTSEYQEKLKFFIENG
                     YVIFESAVSHAQIDRYLTEFDRTLSSESGMLASIPTYGPMDKSMIPAHEATRGAPLTK
                     YLDTYWMIPAALPIVFNRKAQEFISLIFEDGVYAFQGLHFEVGSTQAVHQDTAYVVTE
                     RPRSLAASWCALEDIQPGSGELIYYEGSHRLPEWKYSGKYKHFNHDRDPHQEHMDHLA
                     FLKSESEKRGYPLKRFLPKKGDVLVWAADLAHGGAQIENRSITRKSLVTHFCPTSQKP
                     NYLQYLPKTHQTAREYPGLGKTSTFYYPPGDGPTKMDLGYSITSLLRSVAKRVRRAWR
                     "
     gene            18702..20399
                     /gene="gspE"
                     /locus_tag="PKV56_06585"
     CDS             18702..20399
                     /gene="gspE"
                     /locus_tag="PKV56_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOX67515.1"
                     /translation="MLPPDPPALDVGVHLVREGKLKARDLEHAQSAQTEMGGSLERVL
                     VSLGLVSEADVAVATAQLLKIPFVAAADFPAIPPEVEGLLPDFLVANSVYPLRREAGQ
                     LDVAMASPRDSFVLKALRLSTGLEIRPHAGLPSEIEKALSQLIEDSNTTASRDEFDDL
                     KDSGEFIEHLKDLASEAPVIRLVNAIIGRVTDLRASDIHLEPFEDGLHVRYRVDGVIH
                     SGETIGLKHSAAVSSRIKLLAHLDIAERRLPQDGRIQTRVKGHELDLRLSTVPTVHGE
                     SLVMRVLDRASIRFSLEDMGFEQDNLARFNALLARPHGILLVTGPTGSGKTTTLYAAL
                     SKLDSATQKIITVEDPVEYQLPSINQIQVHPQIGLTFAGALRSILRQDPDIIMIGEMR
                     DGETAQIAVQSALTGHLVLSTLHTNTAAGAVARMLDMGVESYLITSAVNGVLAQRLLR
                     TLCTHCKAPYEPSADLIESAGLARFSAPAKLLYRAVGCEHCRGSGYQGRTGIHELLVL
                     DEPIRKAIIDGVDASVLNGIATRGGMHTLYEDGLLKVAAGITSLEELLRVTQDQSDD"
     gene            20392..21630
                     /locus_tag="PKV56_06590"
     CDS             20392..21630
                     /locus_tag="PKV56_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOX67516.1"
                     /translation="MTDFSWRAADAGGKLVEGRIDASSPAHAMQLLKQRGLLPLRIDA
                     SSKTAQADSTAAGLVRRKGATGKGPVGTSDVLAITSELSVMLRAGLALDNALRVLVEM
                     GHRPAVSDMLQGILEDVKGGKPLSRALENRRKVFGDFYISMIRSGELSGQISTVMERL
                     VEHMERLRALRENVISASIYPAILLGVAILSMIGMLGFVVPQFEKLFVDMGDALPLPT
                     RLVMGLGRGFSEYGVIAALLLLAAVYAGARWLRSTSGRLWWQSSVLRLPILGPLLVKY
                     ELTLFSRSLGTLLGNGVPMVAALNIATETVSNIVLKTALTRVTPLVKSGTRVFDAMSA
                     TQVFEPLAINLMRVGEETGRMGTMMIELANILNRDVETGIKRGLTLIEPALIVVLGLL
                     IGAIIVSILLGILSINDLAV"
     gene            21654..22094
                     /gene="gspG"
                     /locus_tag="PKV56_06595"
     CDS             21654..22094
                     /gene="gspG"
                     /locus_tag="PKV56_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOX67517.1"
                     /translation="MLSPSRFQRKPAAQRGFTLIELLVVLAILTLLIGLVGPRVLGQL
                     GGAKSKTAAVQIADLEKTLELYKLDVGRFPTTEEGLDALIKRPASANGWAGPYLKGGA
                     VPMDPWNKPYRYAATADSQIEIVSFGSDGAAGGEGENADVRNAK"
     gene            22121..22570
                     /locus_tag="PKV56_06600"
     CDS             22121..22570
                     /locus_tag="PKV56_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOX67518.1"
                     /translation="MRSERGFTLVELLVVFAISALLIGLVPTALERLRESVQYRDALR
                     AVMSDLRQAHDRASSQGIEVSFKVDLSRGLYGLEGATPRALPEPLKFRVTVASIELAQ
                     QEVASIRFLPEGGATGGSIDILRPAGNGARLQVDWLSGGTRLLGLPQ"
     gene            22567..22971
                     /locus_tag="PKV56_06605"
     CDS             22567..22971
                     /locus_tag="PKV56_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOX67519.1"
                     /translation="MRRGTQRGFSLLELLVAFSILALSLGVLYRVSGGTVHNLQQSEQ
                     HQRAAALAESLLSLKDAVPPAGWEQSGTSAGYAWRILSTPYSTPAASLNAPRLHQVLI
                     TVAWGGEANPRQIEVVTLLPEQQPIEQAGSAP"
     gene            22968..23648
                     /locus_tag="PKV56_06610"
     CDS             22968..23648
                     /locus_tag="PKV56_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOX67520.1"
                     /translation="MKTRGNPWASRTSHRGFTLVEILVVISLLSVVMLALGAAFRTFA
                     RTEVRIDRRLEVTDEFRIATGFVRSTLGRVSVRKPEPASTSSDRRILLDAQAVSLAWI
                     GVMPARYGAGGLYYFRLAVENIDSENKLVIRYQPCTNTSTFPNWMETEYRALASNITS
                     FEIRYLNIQKSPEEWSTSWLPGDRLPNRVQILVQAAGVDWPDIVVAPRMLPGTGDRSS
                     GFTVGGAR"
     gene            23645..24511
                     /locus_tag="PKV56_06615"
     CDS             23645..24511
                     /locus_tag="PKV56_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOX67521.1"
                     /translation="MNPEAARSARQNGLALVAVLWMVAALSIVVVGMSQGVRGETRRT
                     SLSRQGIVQGALGTAAVNLVIQEMLSSAEPVTRLTYKNVTYQGTEIAVEILPVNGLVD
                     INNASEALLTKLISVQGGMALEQARNLAQATIDIRSVKDSTGQSRGFESVEDWLKVPG
                     IDYTLYARMRNLVSADLFGVGSGRVNALAAPPAVLAVLTGGNTGQALRIASDRDAGRT
                     GIDTTMLNASEIDNASTRRYRISARVPVSTDTQLWTTQTIDLTAIKRDGMPWRILHAE
                     RRYEVTRPERSS"
     gene            24523..25665
                     /locus_tag="PKV56_06620"
     CDS             24523..25665
                     /locus_tag="PKV56_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOX67522.1"
                     /translation="MSTTLPLPDPASSNAPGAWARLGLSMRRRYRESLLDGLTPQVEV
                     RVLLADGSQATWRPDQPLKPLVSPSVAQSSGGLTAIEYPEDRLLRRQLTLPDMPVSEV
                     AQAAELDAVTSSPFRSDDLVWGHRCVPQPSGKLLVDVVLASRRQVQQYLAQQADRLAG
                     RMQPETWVEVWAPMDSSSSQPIFIQGFGESVRARRQARQRWLLGGMFFVAWCLLMGIA
                     ITPTAQLRLRAIEAVAAFDALQHRTAALVLKRESLSKTAEQLAALDAILSERVQAVEV
                     IGVLTRALPDDTSLISLQMQGSKIRISGQTPNTADLMQKLSAYPGLRDVKAPTAATRP
                     LGVNKESFSLELELTPEAFRASEPGRAASAAAIASAPAPKASSPWK"
     gene            25656..26210
                     /gene="gspM"
                     /locus_tag="PKV56_06625"
     CDS             25656..26210
                     /gene="gspM"
                     /locus_tag="PKV56_06625"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOX67523.1"
                     /translation="MEMMFRGKRIPLTLPAWVMLIALSAGSLYYIRLKHDWAADRIQQ
                     IDQRHARLSGLEASRLELEQAEAAARTALSAAIYPASQDVSQAGNEAQRSIREVFSKA
                     GLQVVSSQVLAPKIDRGFDRIPMVVRLEGELAALQGSLMEIPRLSPRVLVDGITVQTI
                     GAVKPDAPQRLAIQLSLHVLRARQ"
     gene            26207..26752
                     /locus_tag="PKV56_06630"
     CDS             26207..26752
                     /locus_tag="PKV56_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67524.1"
                     /translation="MKRYAMQALAALNLGLIVLLAWLWFDQDGQIRDIRWTAPVAIKP
                     DFSNERRSASVQEPATSMALVLATLERPLLSPSRRPPPVVAATTTAEPPADPLANVLI
                     QGIFSGDGIGGIIARVDGIPKRISINESVGSWTLKGIDERNVTFVRKDETRIVQLVRA
                     KEAPPTPAPTTAGGPAPAQPP"
     gene            26890..>28550
                     /locus_tag="PKV56_06635"
     CDS             26890..>28550
                     /locus_tag="PKV56_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOX67525.1"
                     /translation="MLNHRHVYLAVALIASQVSCATADGVPRPRTSVAAEAGAAASTA
                     PSIEAGASTVAGPLASAAASAPGESGAFNKEVTVRGTDRVLAERPAAAPVAGPASSFK
                     FEETPISDVAHVILGQILKVDYVLHKPLNGTVTLATKGSVSADQAAALLEGALQANGL
                     LMARDGRGVYHVGRADALKGIVAAPRQSGNGPLPPGYGAVIVPLENIGAAEMASILRP
                     MLPPDSLVRVDTLRNLLVLVGTRNQAEGWLDIVATFDIDLLKGMSIGVFPLKYASIKD
                     VESALRMITPGAAAAPVGSGGLPGASPALAASQSAPSVETPALLGGVRILPIERINSI
                     IAVTSKAARLEDVKRWIERLDQPTNSSETQLFVYPVQNGSAQHLASVLNGIFGGDSRT
                     TSTPNSTTGVSPGLSSVTAGQGTQQGSSFGYSSSGLTNSQSTSRQSSGNAANSQNVTS
                     VTLAQGTRLMADDLNNAILIYGSRSEYTKIEETLRKLDVPPTQVLIEASIIEVTLNDD
                     LQYGVQWLFNDTSGSGKGTGVLSTVKGGVLGGPLAGFSYTLSNSAG"
BASE COUNT         5142 a         9377 c         9000 g         5031 t
ORIGIN      
        1 tgacctacgc gtagaccagc gaagtgtcct ggaacaggat gatcgtctgc gtcatcagca
       61 ccggcagcat gttgcggaag gcctgcggaa ggaccaccag ctgcatcgtc tgcgcgtagg
      121 tcatgcccac cgcatagccg gcgtacacct gtccccgcgg caccgactgg atgccggcac
      181 gcatgatctc ggagtagtac gctgcctcga acacggtgaa cgtgatgatc gcgctcagct
      241 cggcgcccat cggacggccg atcagcagcg ggatcagcag gaagaaccac aggatcacca
      301 tcaccagcgg gaccgaccgc agcgtgttga cgtacgccgc tgccggcagc accagccact
      361 tcttgcccga cagccgcatc agcgccagca ccgtcccgag ggcgatgccg cccaccatgg
      421 cgatcagcgt cagctggatc gagaagaaga atcccttgag gatgaagctc gagacgacac
      481 cccagtcgag aaagctgaag tccaggttca tcttccgccc ccgatcatcc cgggcacctg
      541 gacacggttc tcgatgaaca gcgcgatgcg gttgatgatg aaggccgaca agaaatacag
      601 gcccgtgacc gccagataga tctcgatgtt gcgcgaggtt tcctcaccgg cctgcaacgc
      661 gaacatcgtc agctcggaga tgctgatggc aaaagccacc gacgagttct tgatgatgtt
      721 catcgactcg ctggtcagcg gcggaatgac gatgcggaac gccatcggca gcagcacgta
      781 gcggtaggtc tgcggcagcg tcagccccat cgccaggccg gcatagcgct ggcccttggg
      841 cagcgcctgg attcctgcct tgacctgctc ggccacccgc gccgaggtga agaaccctag
      901 ggcgaacacc accagcagga aactcggcac ctgcttcaac ggcgggatca gttccgggat
      961 cacgtagtac cagaggaaga cctgcaccag gagcgggatg ttgcggaaca gctcggtcca
     1021 cgcgttgccg atgaacacca gggtccggct gggcgcggtg cgcaggatgc ccatcagcgc
     1081 accgaccgac agcgccacca ccagcgccag cagcgccacg gagagggtcc agccccaggc
     1141 cgacagcagc cagtcgaggt aggtgatgtc accgccggtc ccgaagcagc tcggctggat
     1201 ctctccttcc agggtgctct tgcagaacac ctgccaatcc cagttgctcg ccaaggctta
     1261 cctccttgtt gttctagtca gcatggtgcg gacggcgcgt gctgggtcac ttcttggcgt
     1321 agtcttccat cggcttgtcg ttcgggctgg cccacgcggc cttggtggcg tcggacagag
     1381 gcagtccgac ccgggtgttg gacggcggga tcggttgcag gaaccatttg tcgtagatct
     1441 tggcgagctc gccggactgc atcatgccga cgatcgtcgc atcgaccacc ttcttgaact
     1501 cggggtcgtc cttgcggaac atgatggcaa tgggctcgac cgacagcact tcgccgacga
     1561 tcttgaagtc ggccgggctc ttggacttcg agatcaaccc cgccaggatc tggccgtcca
     1621 tcacgaaggc atcggctcga ccgctttcca tcaacaggaa actgtctgcg tgatccttgc
     1681 cgtagacctc gtcgaagttg atgccgccgg cacgctcgtt cttgcgcagg gtctgcaccg
     1741 aagttgtgcc ggtggtggtg gcgaccttct tgccgttgag ctgggcaatg ccggtgatgc
     1801 cggaactggt ctttgtcgcg atgcgaactt cctcgacgta agtggtcacg gcgaagctga
     1861 cgtccttctg ccgggtggcg ttgttggtgg tcgatccgca ctcgatgtcg acggtgccgt
     1921 tctggaccag cgggatgcgg ttctgcgagg tgaccggctg gtacttcgtg gtcatggccg
     1981 tgatgcccag ctgcttctga atcgccttga ccacgcgctc gcagatctcg atgtggtaac
     2041 cgacgtactt gccatctccg agcgtgtagc tcaaggcccc cgatgattca cgcacgccca
     2101 tggtcacggc ggcggactcc ttgatcttcg acagggtatc ggcctgggcg acaccacagg
     2161 ccagcagtgc agcgaaggcg aaagcggttt tcttcatgac atctccattc gtgatcgtga
     2221 gcggttcccg cgcgcgggcc atgccgcatg tcaacacaac cggcccggtt tgaacagtca
     2281 gccgactacg catatccccg cgcaaggcag cgcggtcctc gaaagcccgc gcaaccttcg
     2341 tgccatgcag gcccgacggg ttcatgcagg cacggcctgc tcatcagggc ccaccgcctc
     2401 gtccgcatcg ccggcaccct gcaggccgtc accgcggctc tgctgcaagc gccacatctc
     2461 ggcgtaccgg ccgttcatcg cgatcagttc ggcatgcgag ccgcgttcga ggatgcggcc
     2521 ctgctccatc accaggatct ggtgcgcgtc gaccacggtg gacagccggt gcgcgatgac
     2581 gagcgtcgtc ttgttgcgcg agacgccctg cagctcggcc tggatggcgc gttcgttcgc
     2641 agagtcgagc gccgaggtgg cctcgtcgaa tatcaggatc ggcgggttct tcagcagcgt
     2701 gcgtgcgatc gcgacccgct gcttctcgcc gcccgacagc ttcaggccgc gctcgccgac
     2761 catggtgtcg tagcccttcg gcgtcgacgc gatgaacgtg tggatgtgcg ccgcctgcgc
     2821 cgccgcctcg acctcgtcgc gccgtgccga cgtgcgtccg taggcgatgt tgtattcgat
     2881 ggtgtcgttg aacagcacgg tgtcctgcgg cacgatgccg atcgcctggc gcaagctctg
     2941 ctgcgtgacc gtgcggatgt cctggccggc gatctcgata cagccggccg acacgtcgta
     3001 gaagcggaac atcaggcgcg cgagcgtgct cttgcccgag ccggacggtc cgaccaccgc
     3061 caccgtcttg ccggcaggga tctcgaagct gatgtcgtgc aggatcgtcc gatcggcgtc
     3121 atagccgaag cgcacgtggc gaaagcgtac ctcggcgcct gctgcggctt gatcgacggc
     3181 cagcgggcgg gcatccggcg catcggcgac ctcacggttc tggcccagca gcgcaaacat
     3241 cttctccatg tcgatcatcg actgcttgat ctcgcggtag atgacaccca ggaagttgag
     3301 cgggatgtag agctggatca tcagcgcatt gaccaggacc aggtcaccca gggtcatcgt
     3361 gccggcgacc acgccttcgg tcgcgcgcca gaccagcaga gtcactgcca ccgcgatgat
     3421 caggctctgg cccaggttca gcaccgacaa cgaggtctgc gacttgatcg aggcgcgttc
     3481 gagccgctgc aggttctcgt cgtagcgcgc ctgctcgaac ttctcgttgc tgaagtactt
     3541 gaccgtttcg tagttgatca gggcgtcgac cgccttggtg ctggccttcg agtcctgctc
     3601 gttcatcgca cgccggaacg cggttcgcca ctcggtcacc gtcacggtga acacgatgta
     3661 cagcagcagg gcactgaagg tgatcgcggc gaaccagcca tcgaacttcg ccgacagcag
     3721 gctgatcacc agcgtgatct cgaccagcgt cggcacgatg ttgtagatca ggtagttcag
     3781 cagcgactgg atcgagcgcg cgccgcggtc gatgtcgcgc gagacgccgc cggtctgccg
     3841 ctcgagatgg aaccgcaggc tcagcgagag caggtgatcg aaggtttcca ggcccacgcg
     3901 ccgggcgatc cgcgccgcga ccggatagaa gaggaattcg cgcaactcgg tgaacagcgt
     3961 gatggacagc cgaagcgcgc cgtagccgat cagcaacgcg accggcacca ccagcacggc
     4021 ctgcgggtcg cccggcttca gcgcgagtgc gtcgaccagg ttcttcagca gcagcggcac
     4081 gccgacgttg gccaccttgg ccgcgatcag gcaggtcagc gccagcgcca cccggtggcg
     4141 ccactgccac acgtaaggca gcagcttctt cagcgtgccc cagtcggagc gcgcgggtcc
     4201 ggccgccgcg tctgcggggg cgggaggcgt cagcgtggca gggcgcatgg gtcggacagt
     4261 agacgggaca gcatgaaaga atcgaccgca gagacaccaa gacaggtcac gcaatatgcc
     4321 ccagatcccg aactcgccgc cggggacacc cgacgacggc actccttcgg tgtcagatcg
     4381 cgcgcacttc gtcagtccgt ccgagctgcc gccggattcg acgctcgtgc tgcgcgtcat
     4441 gccgatgccg gccgacacca acatgagtgg cggggtgttc ggagggtgga tcatggcgca
     4501 ggtcgacatc gccggcgccg tgcttccggc gcgtgtctcg cgcgggcggg tgaccacggt
     4561 ggcggtcaac cgcttcatct tcaagcagcc ggtcagcgtc ggcgacctgc tgagcttcta
     4621 cgcccgcgtc gatcgcatcg gaaccacgtc gatcacggtg catgtcgagg tctatgccga
     4681 acgcaacccg tcgaatccgt acatcgtcaa ggtcaccgac gccaacctga cgtatgtcgc
     4741 catcgacggc cagggccggc ctcgcccggt gcccagagac tgaacgcact gcgcgccgtg
     4801 ctctttcagg agaacgtcga gaagtccggc ttgcgcttct ggaagaacgc actgaaggct
     4861 tcggtggcct cgggactgga cagtcgctga cggaacgccg ccgcctcgat ctcgatagct
     4921 tcgagcaccg cagcctcgct ggcccgccgc atcagctgct tcgactcgcg caccgcgccg
     4981 ggcggcaggc tgttgaatcg ctcggccatg cggcgtgcgt gccgcgccac ttcgcccgcg
     5041 ggcagcacgg cattggcaat ccgcatctcg accgcttctt cggcgctgaa cggatcgccg
     5101 aacaacagtt tctccgccgc cctggcgcgg ccgaccagcg ccggcacgat caggctggat
     5161 gcaaactcgg gcaccagccc gaggctcgca aaaggcatcg ccaggcgcgc ctcgtcggac
     5221 acgtaaacca ggtcgcagtg cagcagcatc gtggttccga tgccgatggc agcgccggtc
     5281 actgccgcga ccaccggctt gctgcaggcg accagcgcgc gcatgaagac gaataccggc
     5341 gcatcgcttg tcgccggcgg tcggttcatg aaatcgtcga tgtcgttgcc cgaggtgaat
     5401 acgccgggct ggccgacgat cagcaccgcg cgcaccgcgg cggcggcatc ggccgcacgc
     5461 agcgcgtcgg ccatcgcgga atacatttcc gcggtcagcg cgttcttctt ttgcggccgc
     5521 gcgatctcga tcgtggcgac gccgttcagc gtcgcggtct tgatgttcat gggttctccg
     5581 gcatcgaatt cagcatcgtt caaggatccc tgcggcacct tgcccggttc ccacgcacat
     5641 cgtcaccatg ccgtacttca ggttgtgacg acgcagcgca tgcaccacgg tggcggcgcg
     5701 gatcgcaccg gtggcaccca gcgggtggcc cagcgcgatg gcgccgccca gcggattgac
     5761 gcgcgccggg tccagatgga gcgcgcccag caccgccagt gactgcgccg cgaaggcctc
     5821 gttcagttcg atccagtcga tgtccttctg ctgcagaccg gcgtagcgca atgcggcagg
     5881 caccgcctcg atcgggccga tgcccatgat ctcgggcggc actcctcggt tcgcgaagct
     5941 gacgaagcgc gccagcggct gcaggttgaa ggtcttgatc gcctgcgcgc tggcgagaat
     6001 caggcagccc gcgccgtcgc tggtctgcga gctgttgcca gccgtgacgc ttcccttggc
     6061 cgcgaacaca gggcgcagcc tggccagtgc ctcgagcgtc gtgtcggcac gcgggccttc
     6121 gtcgaccgcc gcggtgcggg ccttcgacac gacctcgccg gtctgcaggt ccggcgaccg
     6181 gtcggtcacg tcgaccggcg tgatctcgtt gctgaactcg cctgcctcga tcgccttcaa
     6241 cgccttcagg tgcgactgca gtgcgaactc gtcctgcgcc tcgcgcgtga ccttccattg
     6301 ctgcgcgacc ttctcggccg tcatgcccat gccgtaggca atgccgacgt tctcgtcacg
     6361 caggaacacc gccgggttga acgacggctt gttgccgctc atcggcacca tgctcatgct
     6421 ctcgacgcca ccggcgatca tcacctcggc ctcgccgacg cggatgcggt cggcggccat
     6481 ctggatcgca gtcagcccgg aagcgcagaa gcgattgacg gtgaccccgc cgacgctgtt
     6541 cggcagcccg gccagcaagg cgccgatgcg cgccacattg aggccctgct caccctcggg
     6601 catcgcgcag ccgacgatcg catcctcgat cgccttaggg tccagcgtcg gcacttgcgc
     6661 gagggcacca cggatcgccg cgaccagcag gtcgtcgggc cgcgtgttgc ggaacatgcc
     6721 gcgccccgac ttgccgatcg gcgtgcgggt ggcggcaacg atgtaggcgt cttgcaggat
     6781 cttgctcatg ctcttgacct tcagttggag gcgtagcgcc cggacgccag gccaggcggg
     6841 cggcgcgcag gaaccggagc gtacttcctg tacgtgagga ttccgagcac cgaccaacaa
     6901 cgcatggcgt tcgcgcagca gccgatctaa ttgcgcacgg gtttgccggt gctgagcatg
     6961 cccatgatcc gctcctgcgt cttcggctgc tcgagcagcg agcagaaatg ctgtctttcc
     7021 agcgccatca ggtactcctc gctgaccagc gatcccgcat cgacgtcgcc gccgcagagc
     7081 gtctcggcga tcagcgatgc gatgtggaaa tcgtgcgcgc tgatgaagcc accgtcgcgc
     7141 atgttgacga gctgaccctt gatcgtcgcg gcagcgctgc ggcccgccac ggggaacagc
     7201 gtcttcgccg gcggccggta gccgctgtcg aacatggccc tggcctgcgt gagggccacg
     7261 aacagcagct cgtccttgtg cggcacgatc acgtcgctcc acagcagata accaagctgg
     7321 cgactgtcga tcgcgctggt tccgaccttg gccatcgcag ccgccgcaaa tccctccttc
     7381 aggaacgcca gcagatcggc gttcgccccg cccgctcccg ccatctccgc ggcacggcgc
     7441 gcgatgtagg tcagcccgcc ggctcccggg atcaggccga cgccgacctc caccaggccg
     7501 acatagctct ccatcgccgc gacacgcttc gcgcagtgca cggccagttc gcagccaccg
     7561 ccgagcgcca ggccgcggat cgccgccacc accggcaccg tcgcgtagcg cacgcgcagc
     7621 atcgcgtcct gcagcttctg ctcttcgggc gcgatgccct tcgcgccgct cttcatgaac
     7681 accggcatca gcgactcgag gttggcgccc gccgagaaca catcgtcggg cgaccagatc
     7741 accaggccct tgtagctttc ctcagccaga gcgaccgccc tgacgaggcc ttcggtcccc
     7801 gccggactga tcaggtgcag cttggcggtc aggctggcga tcagcacttc gccgtcgagc
     7861 gtccacacgc gcagctcgtc ggtcttgaac agctccgtgc ccgactgcag cggcaccggc
     7921 gcaccggaac cgagcacacg ctcgcggaag tgctggcgcg catagaccgg cagcgcgctc
     7981 tcggcaacga agcgcgactc ggacgcactc cacgaacctt cgggctgatg cacgccctcg
     8041 cggtcggcga ccggcccctt gaacacccag gccggcagcg gtgcggggca cagcgcccga
     8101 ccggcgtcga tgtcttcctg catccagcgc gcgaccgtcg accatccagc tgcctgccac
     8161 agctcgaacg ggccctggct gaatccgaaa ccccagcgca tcgcaaaatc gatatcgcgc
     8221 gcgcactcgg ccacctcggc cagatgcacc gcgctgtagt ggaagccgtc gcgcagcacc
     8281 gcccacagga actgcgcctg cgggtggttc gattcacgca gcagcttcag gcgctcggcc
     8341 ggcggcttct tcaggatgcg accgatcaac tcgtcggcct ggccgccacc ggtcacgtag
     8401 tcgttggcgg cagcgtccca gcgcaggatg tcctttccga ccttcttgta gaaaccggca
     8461 ccggtcttct ggcccagcgt gcccttggcg atcagggcgc cgagcaccgg cggggtggcg
     8521 tagctgccgt agaacggatc gttcgactga tcggggccga ggttgtcctg cagcgtcctg
     8581 atgacatgcg ccagcgtgtc cagcccgacg acgtcggcgg tgcggaacgt gcccgaactg
     8641 gctcggccga gcttcttgcc cgtcagatcg tcgaccacgt cgcaagacag cccgaactgc
     8701 tcggcttcct tcatcaccgc cagcatgccg gccacgccga cgcggttggc gatgaagttc
     8761 ggggtgtcct tggcacgcac cacgcccttg ccgagtgcgg tggtcacgaa ggcttcgagc
     8821 tgatccagga tgtcgggcag cgtggcgggg gtcgggatca gctcgaccag tgccatgtag
     8881 cgaggcggat tgaagaagtg gatgccgcag aaacgcggct ggatcgcctc gggcagcacc
     8941 gcgcccagct tggagatgct cagccccgag gtgttgctgg cgacgatcgc gtgcggcgcg
     9001 acgaagggcg cgatcttcgt gtacaggtcg agcttccagt ccatgcgctc ggcgatcgcc
     9061 tcgatgatca ggtcgcagtc acgcagctga tccaggtgtt cctcgtagtt ggcctgttga
     9121 atcagcaccg cgtcgtcgtt gacaccgagc ggtgcaggct tgagcttttt cagaccgtcg
     9181 accgccttgg tgacgacgcc gttcttcgcg ccttcgcgcg cgggcaggtc gaaaagcacg
     9241 accggtaccc gcacgttggc caggtgggcg gcgatctggg cgcccatgac gccggctccg
     9301 agcacggcga ccttgcggac gggaaatcgt ttcatggtga ctccaggacg ttgggattcg
     9361 gattcggtgg cccgagcaaa tgccgagccg ggctgcggcg ccaccacctc agaacaggga
     9421 ttcgtccatg tccatcaacg gcttggcgcc ggcacgcgcc gtgcggatca gcgcggcggt
     9481 ttccggcagc agcttggcaa agtagaagcg cgcggtggcg agcttcgcac ggtagaacgg
     9541 atcgccattg tccgcatgat cgagcgccac tttcgccatg cgggcccaga agtaggcgaa
     9601 gaccaggtgt ccgacgatgc gcaggtaatc gaccgccgcc gcgccgacct cgtcggcgtt
     9661 gccgaaggcc ttcatgccga gttcggtact cagcttggtg accttgtcgc ccaggtcggc
     9721 cagcggattg atgaattcgc tcatcgcctc gttgacgcct tcgtcctcga tcagctgctg
     9781 caccagcacc ccgaatttct tcagcttcct gccgtggtcg ccgagcacct tgcgccccag
     9841 caggtcgagc gactggatcg tgttggtgcc ttcatagatc atgttgatgc gcgcatcgcg
     9901 cacgaactgc tccatgcccc attcgcgcac atagccgtgg ccgccgaaga cctgcatgca
     9961 gtgcgaggtg gcgatccacg cgttgtcggt gaggaaggcc ttgacgaccg gcgtcagcaa
    10021 cgccaccagg tcggcggcat cctgcttcac cgattcgtcg ctggacgaga gttcctggtc
    10081 gatcagcagt gcggtgaaaa ccgccagcgc gcgcccgccc tcggcatagg cccgtgcggt
    10141 cagcagcatc ttgcgcacat cggggtgcac gatgattgga tcggcgggct tgtcaggggc
    10201 cttcgcgccc gtgagcgaac gcatctggat gcggtccttc gcgtaggccg ccgcgttctg
    10261 gtacgcgacc tcggtcaggc ccagcgactg catgccgaca ccgagccgcg ccgcgttcat
    10321 catcacgaac atcgcgttca gccccttgtg cggcgggccg accagccagc cgcgcgcacc
    10381 gtcgaggttc atctggcagg tggcgttgcc atggatgccc atcttgtgtt cgagcgcacc
    10441 gcagaagatc gggttgcgcg cgcccagcga gccgtcggcg ttcaccagga acttcggcac
    10501 cacgaacagc gagatgccct tgctgccggg cggcgaatcg ggcagccgcg caagcaccag
    10561 gtgcacgatg ttggcggcca ggtcgtgttc tccggcgctg atgaagatct tgtttccggt
    10621 gaggagaaag ctgccatcgg cctgcccgtc cgcctcgaac gggatggctt tcgtgcgcag
    10681 cagccccaga tcggtgccgc aatgcgcctc ggtcaggcac atggtgccgg tccattcgcc
    10741 actggtcagc cggggcaggt aggtctgctg ctgttctggc gtgccatgcg catgcaggca
    10801 ttcgtaggct ccgtgggtga ggcccggata catcgtccag gcctgattcg cgctgttcag
    10861 catctcgtag agacactggt tcaacgcgac cggcaggccc tgccccccat gggccgcgtc
    10921 gcagccgagc gacggccagc caccctcgac aaactgctgg taggcatcct tgaatccagc
    10981 aggcgtggcg acctcgtgcg tgtcacggtt cagcgtgcat ccttcggcgt cgccggtctg
    11041 gttcaacggc gccagcacct cgctcgcgaa cttgccgccc tcctcgagca ccgcatcgat
    11101 ggtctcgatg tcgacctcgg cgtgcggcgg cagcagcttg aaggtgtcgg tgaccttcag
    11161 caactcgtgc aatacgaatc gcatgtcgcg aagcgggggc gtgtagagag ccatggcaat
    11221 tcctgggatc ggtgaaattc attctgcata aagcccgagc acctttcact cgggattgac
    11281 gacgtcccga cctgacgcgg acaagttcat ccggcccagt gtggcaggcc cactgcatcc
    11341 ggacgcaggc atggaggacg acggtcgcgc aggcatgcga atatccgggt tgtcgcaggg
    11401 attggttacg ctcaacgggt aggagtgtcg atcttggaag ttctgcaact cgatgtgtct
    11461 ggacggccgc aatcctggct ctcggccaag gaagctgccg tgctgtatgc cagtgacggc
    11521 gtcgcatgga cgctcggcga cgccttcttc gtgctgcgtg gcggcgttca gcggcgcacc
    11581 ggcgtgcagt cgcgcatcga ggtgcacccc atcatcgcgg tgcgtggcgc catccccagc
    11641 cgcgcctggc gccagagccc cgccctgtcg aacccgaagc tgttcgtccg tgaccgccac
    11701 gtctgcgcct actgcggcgg gcattttcat tcggacgacc tgacgcgcga acacatcgtc
    11761 ccgacctccc gcggcgggca ggacacctgg atgaactgca tcaccgcctg ccgaagctgc
    11821 aacggcaaca agggcagccg gcttccagaa gaagcccgga tgtcgctgct gtacctgcct
    11881 tacgtgccga gcctgcacga ggacatgatc ctgcgaggac gccgcatcct gaccgaccag
    11941 atggaattcc tgctggccag cgtgcccaga cacagcaggc ttcgaacctg actcgccatt
    12001 cccggcaacc ggggcgcagc aacaattctt ttcacccgcc gaaggaacat cggcaggccg
    12061 cacgaatcga agcgctggaa tgcgtcaacg cgaccgcaag ccggcccgtt gtcagatggg
    12121 ctcaaggaat ccaaacatga aaaaagcaat cgcacttctc gccctgctgg gcaccctagc
    12181 tggctgctcg accacgagtc ctgacgtcat ccagcgtggt gacgcgcaac gtctgtcaca
    12241 ggtccaggat gcgaccgtgc tgtcggtgcg tgccgtgacc gtcgacggca gccagagcgg
    12301 cgctggcgcc gcgactggcg cggtcgtcgg tggcgtcgcc ggctcgaccg tgggcggcaa
    12361 caagaccggc gcggtcgtcg gagtgctcgg cgccgtcgcc ggcgccgtgg tcggcaacac
    12421 cgtcgagcgc ttcgccacca aggaggatgc ggtcgaggtg ctgatccaga tgcgcaacgg
    12481 cgaccgccgc gccatcgtgc aggccaaggg caacgagacc ctggccgcgg gcgatgcagt
    12541 ggtcctcgtc acgaccggcg gcaagacccg cgtcatcaag gctcctgcga tcacctcgcc
    12601 gcccccgccg gtcgaaccga agagctgatc ccgccgcgcc tcaggccatc cggggcgcga
    12661 tgtcgaaggc gatgcagatg cgctcctcgg tcgcactgaa tggcacggtg cgatggaaca
    12721 gcgacgacgg gaacaacacg atgtcgccga ccgctggcgg gcagacccgc cgcgggaaat
    12781 ccacgtgtcg tcgagggtag ccgtctccgt cggtgctgaa ctcgatgctg cctgcgtcgc
    12841 tgcccgcgtc ccgcggcggc atggcgaggt acaccgcccc gctgagccag ccggtttcgt
    12901 ggatgtgtga agtcaggtgt ccccccgtct gcatcttcac gtaccacgag ctgctgaact
    12961 cggtggtcgc cggaaagtca cgcgcgaaca caccctcggc tccggcgagc ccctgccgat
    13021 agcgttcgac ctgctcggcg atcagccctg aaagcgcccg aaacgacgct tccgggcgct
    13081 tgaacaggtt cccggacgac tggattccat ggtgcagccg cccctgcttg cgagcggaaa
    13141 tctcggccga agcgatgtcg atcagcagtt gctcgcgcag cgcgctgcgc tcacccgcga
    13201 gggcgtcgat gcggccgtgg gacacgaagt ccagcggatg ggggcagaaa tcgcagtggt
    13261 cggtgacgcc gaaattcgcc gcgtggtgcg ccgacagcgt ggccagaagc ggggcggcgt
    13321 gggcctcggg ggctgcaagc agccgctcca gtccggcccg gaacgcattg aactgttcgg
    13381 tcttgtacag gcagagcaac acccgctgct gccagtcacg cagccgcgaa gcctcgaaat
    13441 agcgggctgc ctggtccggc cggcctctgt cgtaaagcag caccgcgagg ttgtagttcg
    13501 cctcggaggt gtccgggtct tgagccagcg cctgctcgaa gcaggccacg gcctcgtcga
    13561 gacgcccctg gtcacgcagc acctcgccca ggttgttcag cgcgtcgggg tacacaggcg
    13621 ccagcgccag ggcactctcg aaactgcgca ccgattccgc cagccgcccc tcgttgcgat
    13681 acgcggtgcc gagattgaaa tgcacccggg gatcgggctg gatcgccagt gcgctgcggt
    13741 aactgtcgat cgctgcatcc agcgctccct gggcctgcaa cacggccccc aggttgctgt
    13801 gggcttcggc actcgcgggt tgcagcgcaa ggacccgccg gtaactctgc gccgcctcgt
    13861 caaggcgtcc gagggcctgc aacgcagtcc cccggttgta gtgggccacc gccagcgcgg
    13921 gcttgaccgc caccgcctgc gcataactgt cggcagcctc cgcatgccgg cccagctggc
    13981 ccagcaggat gccgagattg caatgcagct cggcgaaatc tggcttaagc gccaccgcct
    14041 tgcgatagct ggccacggca tcctcgcgcc gccccaactg cgaatagagc atgccgagct
    14101 ggctgtgggt ttccatcgac tggggcgcca ccgcggcaac ccgactgaag ctctcgatcg
    14161 cttcggccca acggctctgg ccagccagcg ccgaacccag caggttgagg aagaccagcg
    14221 cctgcggatg cgccgcaaga gccttgcgga cctcggcctc ggcctgcgcc cactgccctg
    14281 cattgagcag cccgacgagc cgctgcacgt cggcagcgct gggctggcgc ccgggagtca
    14341 gggagttctt catggttcct cgcgtggggg ttgatcgggc tattcgccca gcaatgtctt
    14401 caccgtgtcg tacagccgat ccgagccgaa actcggattg gccggatcga acgagaactg
    14461 attgcgcagg acaggggcag agcccgccgc agtgcgggtg tcgagaaaga aggacaggaa
    14521 ctgcgccccg cttgtacggt gatggtcgaa catcctccga gcctcgtcgg gcgccatcgc
    14581 gcgcagcgga tgaaattcgg tcaggccata ggtcttgtgc ttcgagcgcg cataccagtc
    14641 gaagaaggat gttccgtagg tcggctcgcc atagaggctc acacccagat gggcgcggcc
    14701 gaatggtgca agcgaggcat cgaccgcgaa cttgttgacg tcccagcccg ggttggtgaa
    14761 cggcgtgatc tggtggatgt aggtgtcgac ctccgagagg caggatcgcc tcacgcgtgc
    14821 gtcgaaatag cgcaggtaat cgaccacctg ggagcctcga aactcgtgcc acagcggcac
    14881 cagcgggttg tagtagtacg aggaccgctc ggccggcagg tcgacgtaag acagcaccga
    14941 ggcgtcggca ggcacggtga tcggcaacgc cttcgccggc acatcacgtg gcgtcgtctg
    15001 gttgcgatcc atcaccgaaa catgacgggt accgatcagg acgccgttgt cgccgtggcc
    15061 ttcgaggatc agatcgatgc gatgcacgcc aggcgcgagc ccggcgtgat ccagatcgaa
    15121 gcgccagccc acgtcggccg taccgaactc gggtcgcgcc tcgaacacgt cctgacgact
    15181 caatccgacc gcagcacgcc ccacgaattc gccatcccgg tatatgcgca cccagagttg
    15241 ctcacgagcg ccgggtgcca tcaggtggac ccatccggag atcggcagcg tgccatgtgc
    15301 aaacgagtcg atgtgctccg tgaagcgcgt cagcggctgc gagcggatgt ccctggatgg
    15361 cgggtcgact gcgtcgaatg acgggtaatc cgcgcccaca gcctgattca aggcggccac
    15421 gctgccgaac cgagcagcca ggaaggcgcg aaagccggaa atcgacgccg cgctgtgatc
    15481 ggagacgaga tatcgctccc ggaaacccat gccggcctgg aagttcggga acagatgatg
    15541 caattcaccg agcagggtca cgcccttgat cttgtcgcgg tcgtgctgtg gcagacggca
    15601 gatctcgtcg accacggcgt cgatgacctg gacacgccga cgggtgatgc cgttctcggt
    15661 gctcgaaaag gtccaggggt agacatcgct cgcgtaatac cggtcctggg tcatcgatcc
    15721 cacaggtgtc acggccagat tggccggatc ctgcatcagc accttttcga tcggcgaatc
    15781 gacaccgaag tgcgtcgaga agaggtagac gatggccggc cgatcgacgt cgtgcagggt
    15841 ccgaaccagt cgctcgacag ccggctggtc gacgacccag tcgctgccct gctcacgcaa
    15901 caggcgaagc agcggaaact gcagcgtgta gcccagttcg aagcgtccat tcggactgac
    15961 gcggggaccc aggtctgaca gggtgcgctc gatcagcccg gccgccgatc caccttcgcg
    16021 gtcgcactct gaagcaggat ccatggcagc aggggccgcg cgagatggcg cgcccaggca
    16081 agggtcgatc acattcatca tgggagccat cagcagcctg gaaggcggcg ccgggctgtg
    16141 ccacgcgacc caggcgccca acgcgacggc cgtcaaggcg accgcgggcg cagcgacccg
    16201 ccagaacctg cgtgtcatca tcgtcggatt ggatcgatca gcatcacgac aatgctaggc
    16261 cacgcgcctg aaagaacagg actcgccgtg cgcacttcga tttaccgccg ctgcctctca
    16321 cgcacagaca tgttcccgct cccgccgcgc agcttctacg ccacgtcgca cccgaccgat
    16381 tgtgctgccc acggaatgcg gcgcacgcgg ctgcgatgag ccggccggag gtgtcgatgc
    16441 gactcgctga gctctcgtcg ggcaaggaca acaacttcaa cctcattcgc ctgctcgcgg
    16501 caattgccgt gctcgtgagc cacagcatgg tgctggccac gggacatttc agggacaacc
    16561 cctttcccgt cagattgcag acatcgttcg gctcgatcgc cgtcgacgtg ttcttcatca
    16621 tcagcgggtt cctggtcacc gggagcctga ccacccgaca ggacctgagg caatacctgc
    16681 tgtcgcgcgt tctgcggatc tatcctgccc tgctggtgat ggtggcgctg acggtgggcg
    16741 tgctcggaag ctacttcacc accctgccat ggcgctccta ccttgccgac gcagagacct
    16801 ggcgtttcgc actgatgaac gcgaccctgg tcttcggcca tgccgacttc ctgccgggcg
    16861 tgttcgacgc cacgccgatg cgtggactgg tcaacggatc tttgtggacc ataccgcatg
    16921 agttgcgctg ctatctcctg ctgggcggcg tctggcttct ggcttcggct gcccggagtt
    16981 cttgcatgcg ggtctttcgg atggcctgca tcgcggtggc gctggccttt tggatcgcgc
    17041 acccggtcga ccatttctat ctgaagacag gatggccgtt catccagttc ttcttcatgt
    17101 tcatgtcggg ctcgatctat ttcctgctca gggagcacat ccacctgtcg tcgcggctgt
    17161 ttcttgtcat ggtggccgca ctcatcgctt cgactttcca gaacgatgcg ttcttccttg
    17221 tgtatcgatt cacgctgcct tacatcgtcc tgtggttggc attcgtgcca tcgggctcca
    17281 tccgatgctt caacaaggcc ggcgactatt cttatggttt ctatatttat gcattcccct
    17341 tgcagcaatc gatcatttca ttgatgcccc aaacttcaac cctgcaactg atattgatct
    17401 ctggttcggt ggcactgttc ttctctgtat tctcgtggca cacgatagaa aagaaggccc
    17461 ttcaattcaa ggcggcaccg aaataatcag acctacaaac tagccacgaa aacaataacc
    17521 acgcctcgaa atatatattc aaatatttta tttaagtaca tgagcaacaa cgaacccggc
    17581 ggcctttgga tagacaggcc agacgcactg cagattctgg atcagaagaa aaaaagtggc
    17641 attgtcactt ccgaatacca ggagaagctg aaattcttca ttgaaaatgg atatgtcatc
    17701 ttcgagtctg ccgtttcgca cgcacagatc gacaggtatc tcactgaatt cgacaggacg
    17761 ctgagctcgg agtccggcat gcttgccagc atccccacgt acggcccgat ggacaagagc
    17821 atgattccgg cacacgaggc aacccggggt gccccgttga ccaagtatct cgacacttac
    17881 tggatgatcc cggccgccct gcccatcgta ttcaaccgga aggcgcaaga gtttatttcc
    17941 ctgattttcg aggacggcgt atatgccttc cagggtctcc acttcgaagt gggttccacg
    18001 caggctgttc accaggatac ggcctatgtg gtgaccgagc gacctcggtc actggcagcg
    18061 agctggtgtg cgctcgaaga catccagccg ggttcgggcg aactgatcta ttacgagggc
    18121 agtcatcgcc tgcccgagtg gaaatacagc gggaaataca agcacttcaa tcacgatcgg
    18181 gatccgcacc aggaacacat ggatcatctc gccttcctga aatccgaatc cgagaagcgt
    18241 ggttatccgc tgaagcgatt tcttcccaag aagggagatg tactcgtctg ggcggcagat
    18301 ctggcacacg gcggtgcaca gatcgagaac aggagcatca cgagaaaatc actggtgaca
    18361 catttttgcc cgacaagcca gaagcccaat tacctgcaat atctcccgaa gacacaccag
    18421 actgctcggg agtatcccgg tctggggaag acatcgactt tctattaccc acccggcgat
    18481 gggccgacaa agatggacct cgggtactcg atcacttcgc tgctgagatc tgtcgccaag
    18541 cgggttcgcc gcgcctggcg atagctagca acgcatgagg ccacggaccg gcaggcgaag
    18601 ataatcaggg ccgcgccgac ccaccggctc gtgctgtgcc atgacgaatg tgcatgcatc
    18661 gcacttcacg aaggaccccc ttgactccga cgtatcctcc cctgctgcca ccagacccgc
    18721 cggccctcga tgtgggcgtg caccttgtgc gcgaaggaaa gctcaaggct cgcgacctcg
    18781 agcacgccca gtccgcgcag accgaaatgg gcggatcgct cgagcgggtt ctggtgagcc
    18841 tcgggctggt gtccgaggca gacgtcgccg tggccaccgc gcagctgttg aaaatcccct
    18901 tcgtggccgc agcagacttc ccggccattc cgccggaggt cgaaggcctg ctgccggatt
    18961 tcctcgtcgc gaacagcgtc tacccgctgc gtcgcgaggc cggccagctc gacgtcgcga
    19021 tggcctcgcc ccgtgattca ttcgtgctca aggcactgcg cttgtcgacc ggcctcgaga
    19081 tccgaccgca cgccgggctg cccagcgaga tcgagaaagc gctgtcgcag ctcatcgagg
    19141 acagcaacac caccgcatcg cgcgacgaat tcgatgactt gaaggattcc ggcgaattca
    19201 tcgagcacct gaaggacctg gccagcgaag cgccggtcat ccgcctggtc aacgccatca
    19261 tcggccgcgt gaccgacctg cgcgcatcgg acatccatct cgagccgttc gaggacggac
    19321 tgcacgtgcg ctaccgggtc gacggagtga ttcactcggg cgagacgatc ggcctcaagc
    19381 acagcgcggc ggtcagctcg cgcatcaagc tgctggcgca cctcgacatc gccgagcggc
    19441 gactgccgca ggatggtcgc atccagaccc gcgtcaaggg ccacgaactc gacctgcgcc
    19501 tgtcgacggt gcccacggtg catggcgaga gcctggtgat gcgggtcctc gaccgcgcca
    19561 gcatccggtt cagcctggag gacatggggt tcgagcagga caacctggcg cgcttcaatg
    19621 ccctgctggc gcgcccgcac ggcatcctgc tggtcaccgg gccgacggga tcgggaaaga
    19681 cgacgacgct ttacgcggcg ctgtcgaagc tggactcggc gacgcagaag atcatcaccg
    19741 tcgaggatcc agtcgagtac cagctgccgt cgatcaacca gatccaggtg catccgcaga
    19801 tcggcctgac gttcgccggc gcactgcgca gcatcctgcg ccaggatccg gacatcatca
    19861 tgatcggcga gatgcgcgac ggcgagaccg cacagatcgc agtgcagtcc gcgctgaccg
    19921 gtcacctggt gctgtcgacg ctgcacacca acaccgcggc cggcgccgtg gcccggatgc
    19981 tggacatggg cgtcgagagc tacctgatca cctcggccgt caacggcgta ctcgcgcagc
    20041 gactgctgcg cacgctgtgc acgcactgca aggcccccta cgaaccatcg gcggatctga
    20101 tcgaaagcgc cggtctggcc cgcttctcgg cacctgccaa gctgctgtat cgggctgtcg
    20161 gctgcgagca ttgccgcggc tccggctacc agggacgaac cggcattcac gagttgctgg
    20221 tgctggacga accgatacgc aaggcgatca tcgacggtgt cgacgcgagc gttctcaacg
    20281 gcatcgcgac acgcgggggc atgcacaccc tgtacgagga cggcctgctg aaggtcgctg
    20341 caggcatcac cagcctcgaa gaattgctgc gcgtcacgca ggaccagtcg gatgactgat
    20401 ttttcctggc gagcggcaga tgccggaggc aagctcgtcg agggtcgcat cgacgccagc
    20461 agcccggcgc atgcgatgca gttgctgaag cagcgcgggc tgctgccgct gcgcatagat
    20521 gcttcgtcga agacggccca ggccgactcg accgctgcag gtctggtgcg tcgcaagggt
    20581 gcgaccggca agggcccggt cggcacctcc gatgtgctgg cgatcacctc cgagctgtcg
    20641 gtgatgctgc gcgccggcct ggcgctcgac aacgcgctgc gcgtgctggt cgagatggga
    20701 cacaggccgg cggtttccga catgctgcaa ggcatcctcg aggacgtcaa aggcggcaag
    20761 cccttgagtc gcgcgctcga gaaccgccgc aaggtgttcg gcgatttcta catcagcatg
    20821 atccgatccg gcgaactgag cggccagatc tccactgtca tggaacgcct ggtcgagcac
    20881 atggagcgcc tgcgcgcgct gcgcgagaac gtcatttcgg catcgatcta tccggcgatc
    20941 ctgctcggcg tggcgatcct gtcgatgatc ggcatgctgg gcttcgtcgt gccgcagttc
    21001 gagaaactgt tcgtcgacat gggcgacgcg ctgccgctgc cgacccgtct ggtcatgggc
    21061 ctggggcggg ggttctccga atacggggtg atcgcagcgt tgctgcttct cgctgccgtc
    21121 tacgcgggcg cacgctggct gcgctcgact tcggggcgac tgtggtggca atcctccgta
    21181 ctccggcttc caatcctcgg tccgctgctg gtgaagtacg aattgacgct gttttcgcgc
    21241 tccctgggaa cccttctggg aaatggggtt ccgatggttg cggccctcaa catcgcgacc
    21301 gagaccgtca gcaacatcgt gctgaaaacc gcactgaccc gcgtcacccc tctggtcaag
    21361 agcggtaccc gggtgttcga cgcgatgtcg gcgacgcagg tgttcgaacc gttggctatc
    21421 aacctgatgc gtgtcggcga ggaaaccggg cgcatgggca cgatgatgat cgagctggcc
    21481 aacatcctga atcgggatgt cgagaccggc atcaagcgcg gtctgacact gatcgagccg
    21541 gcgctgatcg tggtgctggg cctgttgatc ggcgcgatca tcgtatcgat actgctcggc
    21601 atcttgtcca tcaacgacct ggctgtctga tgcatttcaa caaggatctc ttcatgcttt
    21661 ccccgtcacg attccaaaga aagccagccg cacagcgtgg cttcaccctg atcgaactgc
    21721 tggtggtgct ggcgatcctg accttgctga tcggtctcgt cggcccgcgc gtgctgggtc
    21781 agctcggcgg cgcgaagtcg aagaccgcag ccgtgcagat tgccgacctc gagaagaccc
    21841 tcgagctcta caagctcgat gtcggtcgct ttccgaccac cgaggaaggc ctcgatgcgt
    21901 tgatcaagcg ccccgcctcc gcgaacggct gggccggccc ctacctgaag ggaggtgccg
    21961 tcccgatgga tccctggaac aagccttatc ggtacgccgc cacagccgac agccagatcg
    22021 agatcgtgtc gttcgggtcc gatggtgccg ccggcggcga aggcgagaac gccgacgtgc
    22081 gcaacgcgaa gtaactgccg gcggttgccc ggggcccgtc gtgcgcagtg aacgcggctt
    22141 cacgctggtc gaactgctgg tggtattcgc catctcggcg ctgctgatcg gactggttcc
    22201 cacagccctc gagcgcctgc gcgaatcggt gcagtaccgg gacgcgctgc gtgcggtcat
    22261 gagcgatctc cggcaggcac acgaccgcgc gtcctcgcag ggcatcgagg tcagcttcaa
    22321 ggtcgacctg tcccgcggcc tttacgggct cgaaggtgca acacctcgtg cgctgcctga
    22381 gccgctgaaa ttccgcgtga cggtggccag catcgagctt gcgcagcagg aggtcgcttc
    22441 gatccgcttc ctgcccgaag gtggtgcgac cggaggcagc atcgacatcc tgcgacctgc
    22501 cggaaacggc gcacggctgc aggtcgattg gctgtcgggt gggacccgct tgctcggtct
    22561 gcctcaatga ggcgcggaac gcagcgcggc ttctcgttgc ttgaactgct ggttgcgttc
    22621 tcgatcctcg cgttgtcgct cggcgttctc tatcgggtca gcggtggcac ggtgcacaac
    22681 ctgcagcaaa gcgaacaaca ccagcgcgcg gcagcattgg ccgaatcgct gctcagtctc
    22741 aaggacgccg tgccaccggc tggatgggaa cagtctggca cctctgcggg atacgcatgg
    22801 cgcatcctca gcactccgta ttcgacgccg gcggccagcc tcaacgcacc ccgtctgcat
    22861 caggtcctga tcacggtcgc ctggggtggc gaggccaacc cgcgccagat cgaagtggtg
    22921 accctgcttc cagagcagca gcccatcgag caagcgggat ctgcgccatg aagacgcgtg
    22981 gcaacccatg ggcaagtcgg accagccacc gcggattcac gctggtggaa atcctggtcg
    23041 tgatttcgct gctttcggtg gtgatgctcg cgctcggtgc agcgtttcgg accttcgccc
    23101 ggaccgaagt ccggatcgat cgacgcctcg aggtgacgga cgaattccgg attgccacgg
    23161 gattcgtcag aagcaccctg ggtcgggtat ccgttcgaaa gcccgaacct gcatcgacct
    23221 cctccgaccg gcggatcctg ctcgacgccc aggccgtatc gctggcctgg atcggcgtga
    23281 tgcccgcgcg atatggggca ggcgggttgt actatttcag actggccgtc gaaaacatag
    23341 attccgaaaa caagctcgtg atcagatacc aaccctgcac gaacacctcg acatttccaa
    23401 attggatgga aactgaatac agggcactgg catccaatat cacctcattc gaaatcagat
    23461 acctgaacat acagaaatcc ccagaggaat ggtccaccag ctggctgccc ggggaccggt
    23521 tgcccaatcg ggtgcagata cttgttcagg ccgcaggtgt cgactggcca gacatcgttg
    23581 tggcgcctcg catgctccct ggcacaggcg atcgatcgag cgggttcacc gtcggaggtg
    23641 cgcgatgaac cctgaggcag ccagaagcgc gcggcagaat ggcctggcac tggtcgccgt
    23701 gctctggatg gtggccgcgc tgagcatcgt cgtggtgggc atgtctcaag gggtccgcgg
    23761 tgaaacacgc aggacctccc tctcgcggca gggcatcgtc cagggagccc tgggaaccgc
    23821 agccgtcaac ctcgttattc aggaaatgct gtcttcggca gagcccgtca cgcgactcac
    23881 atacaagaat gtgacctatc agggcactga aatagcagtc gagatattgc ccgtcaatgg
    23941 cctcgtcgat atcaataatg cttctgaagc gctgctgacg aaactgattt ccgtccaggg
    24001 cggcatggcg ctggagcagg cccggaatct ggctcaagca acgatcgata ttcgttccgt
    24061 aaaggattcg acgggacaga gccggggttt cgaatcggtc gaggactggc tgaaggttcc
    24121 cggtatcgac tacacgctgt acgctagaat gaggaatctc gtcagtgccg acctgtttgg
    24181 agtcggctcc ggacgggtca atgcgctggc agccccacct gccgtgctgg ccgtattgac
    24241 tggcgggaac accgggcagg ccctgcgcat tgcttctgat cgagacgccg gccgcaccgg
    24301 catcgacacg accatgctca acgccagcga gattgacaac gcttccacgc gccgttaccg
    24361 aatctcggcc cgggtgcctg tatcaaccga cacgcaactt tggaccaccc agaccatcga
    24421 cctcactgcg atcaagcgcg acggcatgcc atggcgcatc ctgcatgctg aacgccgcta
    24481 cgaagtgacg cgtcccgagc gctcgagttg acagcacctc tcatgtccac gactttgccc
    24541 ctgcccgacc cagcgagctc gaatgccccg ggcgcttggg cgcgcctcgg cttgtcgatg
    24601 cgccgccgct accgcgagtc actgcttgac ggactgacac cccaggtaga agtgcgcgtg
    24661 ctgcttgccg atggttcgca ggccacgtgg cgccctgacc agccactgaa gccgctggtc
    24721 tcgccttcgg tcgcccagtc cagcgggggg ctcaccgcga tcgagtaccc cgaagatcgc
    24781 ttgttgagac gccagctcac gctgcccgac atgcctgtga gcgaggtcgc gcaggccgca
    24841 gagcttgatg cagtcacctc cagccctttt cgttcggacg acctcgtatg gggtcatcgc
    24901 tgcgtgccgc agccttccgg gaagttgctg gtcgatgtcg tgctcgcgtc acgcaggcag
    24961 gttcaacagt acctcgcgca gcaggccgat cgacttgcgg gtcggatgca gcccgagacc
    25021 tgggtcgaag tatgggctcc gatggacagt tcgagttctc agccgatctt catccagggc
    25081 ttcggtgaga gcgtccgcgc tcggagacag gcacggcagc ggtggctgct gggcggcatg
    25141 ttcttcgtcg cctggtgctt gctgatgggc atcgcgatca ccccgacggc acagctgagg
    25201 ctgcgggcca tcgaagcggt cgcagcgttc gacgcactcc agcacaggac tgctgcgctg
    25261 gtgctcaagc gggagtccct gtcgaagacc gccgaacaac tcgctgcgct ggacgcgatc
    25321 ctgtcggaac gggtgcaagc ggtcgaggtc atcggtgtgc tgacacgtgc actgcctgac
    25381 gacacgtcat tgatcagcct tcagatgcag ggctcgaaga tacggatttc gggtcagacc
    25441 cccaacaccg cagacctgat gcagaaacta agcgcctacc cgggcctgcg ggatgtcaag
    25501 gcgccgactg ccgccacccg accgctcggc gtgaacaagg aaagtttctc gctcgagctc
    25561 gagctgacac cggaagcctt tcgggcgtcc gagcccggcc gcgcggcttc ggcagcagcc
    25621 atcgcgagcg cgcccgcgcc gaaagcgtct tccccatgga aatgatgttt cgaggcaagc
    25681 gcattccgct gaccctgccg gcctgggtga tgctgatcgc gctttcggcg ggcagcctgt
    25741 actacatcag gctcaagcac gactgggccg ccgaccgcat ccagcagatc gatcagcgcc
    25801 acgcgcgcct gtcgggcctc gaagcgagcc ggctcgagct cgagcaagcc gaagcagcag
    25861 cgcgtaccgc gctttctgcg gccatctacc cagcgagcca ggacgtgagc caggcgggca
    25921 acgaggccca aagaagcatc cgcgaagtgt tctccaaggc cgggttgcag gtcgtgagca
    25981 gccaggtgct cgcaccgaaa atcgaccggg gtttcgatcg cattccgatg gtggtgcgac
    26041 tcgaaggcga actggcggca ctgcaaggct cgctcatgga aatcccgcgg ctttcgcctc
    26101 gggtgctggt tgacggaatc acggtccaga ccatcggtgc ggtcaagccc gacgcgccgc
    26161 agcggctggc catccagttg agtctccacg tgttgagagc gcgccaatga agcgctatgc
    26221 gatgcaggct ctggccgcgc tgaatctggg gctcatcgtg cttcttgcat ggctctggtt
    26281 cgaccaggat ggtcagattc gcgacatccg ctggacagcc ccagtcgcca tcaagccgga
    26341 cttctcgaac gaacggaggt ctgcgtcagt tcaggagccg gccaccagca tggcgttggt
    26401 gcttgccacc ctggagcgcc cgctgctctc gcccagcaga cgaccgccac cggtcgttgc
    26461 cgcgaccacc acggccgagc ctccagccga tccgctggcc aatgtcttga tacaaggcat
    26521 cttctcgggc gacgggatcg gcggcatcat cgccagggtg gacggcatcc cgaagcgcat
    26581 ctcgatcaat gaatccgtcg gcagctggac cttgaagggc atcgacgagc gcaacgtgac
    26641 attcgttcgc aaggacgaga cacgcatcgt tcaattggtg agggcaaaag aagccccacc
    26701 cacacccgca ccgaccactg cgggcggccc cgctccggct cagccaccct gaccgcaagc
    26761 catggcggcc ggcagcacgg cagcagcaca agccgcgcaa gatgcagtgc gcgagcgtta
    26821 tcgccgacgc aacgaagtcc gggccaaggc gggactgcct cccgtttccc tcccataacg
    26881 gcagcaaaca tgctcaacca tcgacacgtc taccttgctg ttgcattgat cgcaagccag
    26941 gtttcctgcg ccacggcaga cggcgtcccg cgaccccgga catcagtggc cgcggaggct
    27001 ggcgcggccg catcgacagc cccctcgatc gaggccgggg ccagcacggt agccggacca
    27061 ctcgccagtg ccgcagcgag cgcgcccggt gagtccggcg ccttcaacaa ggaggtcact
    27121 gttcgaggca ccgatcgtgt gcttgcagaa cgcccggctg ctgcaccagt cgcaggaccc
    27181 gccagttcgt tcaagttcga ggaaacgccg atctccgacg tggcgcacgt gatcctgggg
    27241 cagatcctca aggtcgatta cgtgctgcac aagccgctca acggcacagt gacgctggcc
    27301 accaaaggca gcgtgtctgc cgaccaggcc gccgcgcttc tcgaaggtgc gttgcaggca
    27361 aacggactgc tcatggctcg agacggacgt ggcgtctatc acgttggtcg ggcagacgcg
    27421 ctcaagggca tcgtcgccgc tccgaggcag agcggcaatg gtccgctgcc tcccggttac
    27481 ggcgcggtga tcgttccact cgaaaacata ggcgcagcgg agatggcttc catcctgcgt
    27541 ccgatgctgc cgccggactc tctggtccgc gtcgataccc tgcgcaacct gctggtgctc
    27601 gtcggcacca gaaaccaggc cgaaggctgg ctcgatattg ttgcgacctt cgacatcgac
    27661 ctgctcaagg gaatgtcgat tggtgtcttt ccactgaagt acgcatccat caaggacgtc
    27721 gagtctgcat tgcgcatgat caccccgggc gctgccgcag cacccgttgg atcgggaggc
    27781 cttcctggcg catcccctgc gctggccgcc tcacagtccg ccccctcggt tgagactcct
    27841 gccttgctcg gcggcgtccg gatactgccg atcgaacgca tcaacagcat catcgccgtc
    27901 acctccaagg cggcgcgact cgaagacgtg aagcgctgga ttgagcggct cgatcagccg
    27961 accaacagct cggagacgca gctcttcgtg tacccggtgc agaacgggtc tgcacaacat
    28021 ctggcttcgg tattgaatgg catcttcggg ggcgattccc gcaccacgtc cacgcccaat
    28081 tcaaccactg gcgtgtcgcc agggctttca tccgtcacgg ctggacaggg cacgcaacag
    28141 ggttcgagtt tcggttactc gagctcgggg ctgacgaact cgcagtccac atcgcgccag
    28201 agcagcggca acgccgcgaa ttcacagaac gtgacctcag tgacactcgc ccaaggcaca
    28261 cggctgatgg ccgacgacct gaacaacgcc atcctgatct atggcagccg cagcgagtac
    28321 acgaagatcg aggaaacgct gcgcaagctg gatgtgccgc ccacgcaggt tctgatcgaa
    28381 gccagcatca tcgaagtcac gctgaacgat gacctccagt acggagtgca gtggttgttc
    28441 aacgacacgt ccggcagcgg caagggcacc ggggtgctca gcactgtcaa aggaggtgtc
    28501 cttggcgggc ccctggctgg cttctcgtac accctcagca actctgctgg
//
LOCUS       DAOORV010000034        28278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_1942_length_28278_cov_9.036105, whole genome shotgun sequence.
ACCESSION   DAOORV010000034 DAOORV010000000
VERSION     DAOORV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28278
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_28278_cov_9.036105"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKV56_06640"
     CDS             complement(<1..464)
                     /locus_tag="PKV56_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX67526.1"
                     /translation="MSIQTDEFEPRRVVSAAPASPNEEAIERALRPKGLTEYVGQAKA
                     REQLEIFIGAAKMRSEAMDHVLLFGPPGLGKTTLSHIIANELGVNLRQTSGPVLEKPK
                     DLAAILTNLERNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARS"
     gene            805..1284
                     /locus_tag="PKV56_06645"
     CDS             805..1284
                     /locus_tag="PKV56_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOX67527.1"
                     /translation="MNKLSIQRLIAVTALSFAALGVTAHSFKVGNIDIGHPYARPTSS
                     GQQVGAGYLKVTNRGAADRLLSATAPVAASVEMHSMVMEGDVMKMRQVDAIDLPAGQT
                     VELKPGGYHLMLMGLKAPLKDGDKLPLTLKFEKAGVVVVTVNVESPKPSEAAAEHKH"
     gene            complement(1316..1885)
                     /gene="ruvA"
                     /locus_tag="PKV56_06650"
     CDS             complement(1316..1885)
                     /gene="ruvA"
                     /locus_tag="PKV56_06650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOX67528.1"
                     /translation="MIGRLTGTLAEKSPPQLLIDVSGVGYEVDVPMSSFYNLPGLGER
                     VTLLTHFVVREDAQILFGFLTHEERQTFRQLIRITGVGPRMALSLLSGLSVGELAQAV
                     ARQETARLVKVPGIGKKTAERLLLELKGKLADAISAPASIADDAQADILQALIALGYS
                     DREAAAALKNLPAGTSVSDGIKLALKSLG"
     gene            complement(1922..2581)
                     /locus_tag="PKV56_06655"
     CDS             complement(1922..2581)
                     /locus_tag="PKV56_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67529.1"
                     /translation="MNNILLLEDLPEIRAWMKALVLQVFPKSHIHEAARIHDALELTT
                     AIKFDLALVDLGLPDGSGVTVVAELRENQPEAQSVVVTIHDDDDHLFPALQAGAFGYL
                     LKEQSRELIAEQLKRMSGGEPPLSPSIARRVISHFAAQSRSKPPQQAHAMPHVSLTDR
                     ENEVLLRVAKGYTLPEIGVQLSLSRHTIADYVKQIYRKLNVSSRAEAALEAQRLGLFR
                     S"
     gene            complement(2655..4484)
                     /locus_tag="PKV56_06660"
     CDS             complement(2655..4484)
                     /locus_tag="PKV56_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67530.1"
                     /translation="MPTRALFLILALWAWLVTGAAHAEALTIHSALSINSNEAHFPTQ
                     GTPAPVRLPDDWAASRPDHDGSVWYRASFVRPAGLAPDDLLALYIERACSNLEVHLNG
                     RRIFSGGRMAEPTARNCHRPQLVNLPAVLLDQPLNVLDIQVHGSALRHVSARARAGGL
                     SVLRIGPASEMKALYEQREFWSIRTVQIIGITLIALGGFLLILSWINRSEIFLMYFGW
                     LLIGWTVLSLRVWWPQWPWETHTFEIVVCSAFPPVVAMAVQFLLSYAGVRSRPVAIAL
                     LLQCMLVPISLAAVGADHLFTSASAWFMLMAAELIAAIAVYLWLAWKNHRDDFWPMGI
                     TLGCMGLLVMNEIAMQYGWLPRLAVQPLNFAFPLLLLGIGSRLLQVFGRALQAAEVNR
                     TLLEKRVQEATAEIERNFAQLSALRVEQVTEKERKRIAADLHDDLGAKLLTIVHTSES
                     ERISTLAREALEEMRLSVRGLTGKPVRLADALADWRAETVSRLGQANIEADWRNPVDE
                     LEQQLTARVFVQTTRILREAVSNIIKHSGASHCKVRTSVDAQHFGLLVQDNGKGIAME
                     LDGKLDRGHGMSSMKHRAKQLLGQCLIESGPGYGTVIRLTIPL"
     gene            4589..5590
                     /locus_tag="PKV56_06665"
     CDS             4589..5590
                     /locus_tag="PKV56_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOX67531.1"
                     /translation="MILRHAFIPLDNARLAHLCGSLDCHLRDIESAFDVTITRRNESF
                     RIEGGKAQAERAVALLQSLYDRAQRPLADDAFQLSLVEAAYDSAGRAIPQAPLAAAGG
                     PAPEVVPAESRSALRTRRADLAGRTPNQHLYLANIQSHDITFGIGPAGTGKTFLAVAC
                     AIDALERSSVQRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGTDKVT
                     RAFEKGIIEVAPLAFMRGRTLNNAFVILDEAQNTTPEQMKMFLTRIGFGSKCVVTGDV
                     SQIDLPRGQMSGLIDAERVLRRVRGVATTRFSSADVVRHPLVARIVDAYEASRADTV"
     gene            5595..6050
                     /gene="ybeY"
                     /locus_tag="PKV56_06670"
     CDS             5595..6050
                     /gene="ybeY"
                     /locus_tag="PKV56_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOX67532.1"
                     /translation="MSRPTLALSLQFAREDLPRAHRCHLPRHRVARWIRAALDAPADI
                     TVRIVGSEEARALNREFRGKDYATNVLTFDYSHAPQVHADLVLCADVVEAEARAQSLP
                     VEAHYAHLLVHGALHAQGHDHESDDDAERMESRESAVMVALGFADPYAD"
     gene            complement(6072..7190)
                     /locus_tag="PKV56_06675"
     CDS             complement(6072..7190)
                     /locus_tag="PKV56_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOX67533.1"
                     /translation="MRVFHIQGDQFTELETLPEKLPPAGYLWVGSARREFEVQSSVLQ
                     AALQGWTGGQLVDLHISDLLNNQLPSHFDYTSWYDVLVFRRLAAGEGSAPMFLDDSKG
                     TLATARAALAAIDTSPVGFALFDRVLLTVHPTDCQVREFFANRLQQQALPAGSDARML
                     EGRNAARLPASAADLMLRMVNHMVDSYLELRRLLTKQLTYLQQELFNPRSRFDNWQML
                     LESRNALHLLEDTCEDQRSAITEWIDALDEWPTDADKNARRERELLRVRSRDVLEHIE
                     RVLSHVRRLESSAETAVQMHFSAQSNRTNDIMRTLTVLTAIFMPLNLVTGFFGMNFEG
                     LPLIHSMSGFWAVFMAMLVLGIGLSVFFWRKRYLGSKH"
     gene            complement(7210..8214)
                     /gene="hemB"
                     /locus_tag="PKV56_06680"
     CDS             complement(7210..8214)
                     /gene="hemB"
                     /locus_tag="PKV56_06680"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOX67534.1"
                     /translation="MNTPPAYPTSRPRRLRKDEFSRAMVREHRLHPSDLILPVFVTEG
                     HDRAQDVASMPGVRRLTLDRLLLVAEDCVNLGIPAIALFPVIETALKTEDGREALNPA
                     GLVPRVVRELKRRFPELGVITDVALDPYTSHGQDGLLDAAGYIVNDPTVDVLRQQALV
                     HAEAGVDIVAPSDMMDGRIGVIRQALESGGHVHTKIMAYSAKYASAFYGPFRDAVGSA
                     GNLGKSNKKVYQMDPANTDEALREVALDIAEGADMVMVKPGMPYLDIVRRVKDEFHVP
                     TFAYQVSGEYAMLKAASANGWLDHDAVMMESLLAFKRAGADGVLTYFALDAARLMQAG
                     "
     gene            8389..8811
                     /locus_tag="PKV56_06685"
     CDS             8389..8811
                     /locus_tag="PKV56_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOX67535.1"
                     /translation="MLWIKAFHIVFVSAWFAGLFYLPRIFVNLAMVPADSHAERERLL
                     LMARKLYRFTSLLMVPALGLGIWLWLAYWRTGAGGWLHAKIALVVLVVGYHHMCRGLL
                     RQFEQFANQRSDRWFRVFNELPVILLIVIVVLVVVKPF"
     gene            8845..9954
                     /locus_tag="PKV56_06690"
     CDS             8845..9954
                     /locus_tag="PKV56_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOX67536.1"
                     /translation="MARHRSSAWLLAWLYAGLIAYASLYPFAGWRWPTVWPLAFLTLP
                     WPRYWTGFDLVSNLLGYLPLGLLVFAAQVRTGRNAGGAFLRAVLSAAAASLLMEMVQN
                     GLPSRVPSNVDLAFNTLGAAAGAVLGALMHVVGLLDRWQRLRDRWFVPQSAGGLTLLV
                     LWPIGLLFPSALPFGVGQVLSRLQDTLAGWLDQTPLAEWSRNILEAQWFGPGLPPAGE
                     FCAIALGLIAPSLLAFTVCRSGWRRSVIALGVAGAGVGATTLSTALNFGPDHATAWLS
                     STAVAGLGAGALASLLLAWVPRRASAALGLMALTALVSLISQASEDVYFAQSLQAWEQ
                     GRFIRFHGAAQWVGWLWPYAAMAYLLGRIAARDED"
     gene            10044..10370
                     /locus_tag="PKV56_06695"
     CDS             10044..10370
                     /locus_tag="PKV56_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOX67537.1"
                     /translation="MSYFNRHVFFCLNQRDAGEESCARQGAQAGFDRCKSRVKEAGLS
                     GPGQVRVNKAGCLDRCSGGPVLVVYPEAVWYTFVDNDDIDEIVDSHLKNGQVVERLLI
                     PPSVGK"
     gene            10408..11031
                     /locus_tag="PKV56_06700"
     CDS             10408..11031
                     /locus_tag="PKV56_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX67538.1"
                     /translation="MNSQTQSAQVDGPAGSLQCALDMPATPAIGVAVVCHPHPQFGGT
                     MDNKVVQTLARALLQLGWATVRFNFRGVGKSAGAWDEGRGEVDDALAVIERHRIAGQP
                     LLLAGFSFGAYVAAEAASRLPEAQRPLRLVLVGPSTQKQCIPAVPADTVVIHGESDEV
                     VPLSATLDWARPQSLPVIVLPGVGHFFHGQLGWLKTVVVQQLQLAPR"
     gene            11090..12295
                     /locus_tag="PKV56_06705"
     CDS             11090..12295
                     /locus_tag="PKV56_06705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOX67539.1"
                     /translation="MNFSLRSLSRSFSAGLLLIAGVFALSTAQAQMPQPPEVAARSYL
                     LVDLTSNQTLAERDADAPSDPASLTKLMTAYVVFAALRDKKLALDQKLSISMRAWEER
                     KGGGSLMFIDTTMTPTVDELLRGMIVQSGNDASVALAEAVGGTLDQFVAMMNRQSQAW
                     GLKNTAFKNVTGLTEPGHRSTARDLSVIASHIIRDFPKEYAYYSMKEFRYNNITQPNR
                     NLLLRRDPSVDGMKTGYTDAAGYCLIASAQREFPNLGAGGNGGGPRRLLSVVLNTVSM
                     EARANESQKLLNWGYQAFETVRLFEVNKAVATVPVWKGQTDQAQLGANGALFVTMPRG
                     EGGKLQTKIERTDPLVAPLLQGQRVGTIKVSTAAGTAVTEVPLVVLQPVEAAGVFGRA
                     WDALRLWIK"
     gene            12334..13209
                     /locus_tag="PKV56_06710"
     CDS             12334..13209
                     /locus_tag="PKV56_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HOX67540.1"
                     /translation="MPNLPDTLCYLDGNYTPLNQAKVSVLDRGFLFGDGVYEVVPVYG
                     RGLFRFEEHMARLERSLAKVRIANPHTAAEWLARCRRLIESHVAEDQLIYIQVTRGVA
                     PRDHVMPTDIEPTVFIMVSALKQPSAEQRHRGVACVTARDFRWERGDIKSISLLGNVL
                     ARQISADHGAVETILFRDGFLTEAASCNVWVVHEGAVLGPPASEHVLEGVRYELIREL
                     CDDVGIAFNLRPIPEGEVVAADELMLSSASKEVLPVTMLDGESVGHGVGRGKPGPVYA
                     KLYAAYQRAKAAQRL"
     gene            13221..13496
                     /locus_tag="PKV56_06715"
     CDS             13221..13496
                     /locus_tag="PKV56_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOX67541.1"
                     /translation="MQDIPREPSLIEYPAQFPIKVMGGNVDGFTEAVLEVVRQFDPAF
                     DPQSIEKRSSSGSKYLGLTVTVTATSRVQLDDLYRALSSHPLVKVVL"
     gene            13512..14219
                     /gene="lipB"
                     /locus_tag="PKV56_06720"
     CDS             13512..14219
                     /gene="lipB"
                     /locus_tag="PKV56_06720"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOX67542.1"
                     /translation="MTAPTLACLGLVDAAETIEAMRACVELRNAALLPTPDEIWLCEH
                     LPVYTQGLAGKPEHLLDPGDIPVIQTNRGGQVTYHGPGQVVAYPLIDLKRLGIYVKEY
                     VYRIEHCVIKTLESYGVTGHRVPGAPGIYVRLEDPGGHAALESVPPGERRGVAAPDPF
                     RGLGKIAALGIKVSRHHAYHGVALNVAMDLSPYQRINPCGYPGLATVDLATLGVHADV
                     ASVSRQLGARLAEHFTP"
     gene            14271..15269
                     /gene="lipA"
                     /locus_tag="PKV56_06725"
     CDS             14271..15269
                     /gene="lipA"
                     /locus_tag="PKV56_06725"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOX67543.1"
                     /translation="MSSDSVSPVASDASAYDASAKQKSQAKTSRIPIKIVPAEALKKP
                     DWIRVKAGSPSTRFYEIKQILREQQLHTVCEEASCPNIGECFGKGTATFMIMGDKCTR
                     RCPFCDVGHGRPDPLDANEPLNLARTIAALKLQYVVITSVDRDDLRDGGAGHFVDCIR
                     QTRALSPATRIEVLVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYA
                     FSLNLLKKFKAMHPEVPTKSGVMVGLGETDEEILQVMRDMREHDIDMLTIGQYLAPSG
                     HHLPVRRYVHPDTFKMFEVEALKMGFTHAAVGAMVRSSYHADQQAHAAGVADALPR"
     gene            15298..15996
                     /locus_tag="PKV56_06730"
     CDS             15298..15996
                     /locus_tag="PKV56_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOX67544.1"
                     /translation="MQRRSFTSGLLSGVSLCSVGIAPAAHAASVLSDADASSGIRAAL
                     ERGAEAAVSLLGKPDGFLGNPKVKIALPGFLKDGAKLLKATGQGKRVDELVTAMNRAA
                     EAAVPEAKELLLGAVKSMSVEDGARILSGGDNSVTDFFAQKTREPLGVKFLPIVTQAT
                     EKVSLAEKYNAVAGKVAGLGLVDKKDANIQQYVTGKALDGLYLMIAEEERKIRKDPLG
                     TGSDILKKVFGSLK"
     gene            complement(16043..17185)
                     /locus_tag="PKV56_06735"
     CDS             complement(16043..17185)
                     /locus_tag="PKV56_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67545.1"
                     /translation="MGSIETVGKSASASGLPIPAGQGEERWRQVIAELGAEIAHPLTA
                     ALERIQALISSGRIDRSGLRSLRDEIEAARQVGMTAQLLARFANARMQQSRERVALAD
                     SLQTVLTHRKRETEARGIELQPVLNPVDVIVDASLAFSLLNTLLDWAMVHARSRIEFT
                     IELQSWKSRARLNCRFELPSAQETADGADAPAQPALDSLTWRLLEQTAATMNLGLSRQ
                     VADGAVTVQIEFTAAARSEIDGITMVDLDEGFDSTMNSKPLAGSHVVVVASRREVRAR
                     VRDALRDMGLLVDMVGSIDEAADFCRDGLPHAIIIEGILRGDRCERFCAEIRAEVPGF
                     PFIDIIEQGREFNVSDAERGERARVGQDAIEAALPSVLMFELSKTL"
     gene            17679..18137
                     /locus_tag="PKV56_06740"
     CDS             17679..18137
                     /locus_tag="PKV56_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HOX67546.1"
                     /translation="MPGLLIIAHAPLASALKSVAGHTFPDCAAPLEVLDVPPDMPLEQ
                     IEHLARELLARVRNPEALILTDVFGATPCNVAQRLAGLAEPGQVKVVTGVNVPMLWRS
                     LCYCHESLDAVVARAVAGATQGVMQVSISRPQNQTYKPGSHDPNQHHHQQ"
     gene            18106..18375
                     /locus_tag="PKV56_06745"
     CDS             18106..18375
                     /locus_tag="PKV56_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOX67547.1"
                     /translation="MIQTSITISNKLGLHARASAKLTKLAGGFRSDIFISRGDRRVNA
                     KSIMGVMMLAAGMGTEVQIDASGDDEQAAVDALAGLIHDKFGEGE"
     gene            18458..20230
                     /gene="ptsP"
                     /locus_tag="PKV56_06750"
     CDS             18458..20230
                     /gene="ptsP"
                     /locus_tag="PKV56_06750"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOX67548.1"
                     /translation="MSFQLFGLPVSRGVAIGRAVLISSSRVDVAHYFIRDADVAPEIE
                     RLRSARDAVASELSALQRDIPGDAPAELSALLDVHLMLLNDETLAIATKQWIRERHYN
                     AEWALSAQLEVLARQFDEMEDDYLRERKADVEQVVERMLRAMARGSASLLTDAATPTQ
                     GGTPHSEPLVMVANDIAPADMLHFKRTVFAGFVTDVGGKTSHTAIVARSLDIPAVVGA
                     REGSRVIRQDDLVVVDGDAGLVIVNPSDIVLEEYRFRQRECELAQQRLSRLRHTPAVT
                     LDGERIELMANIELPGDAAAAVAAGAVGVGLFRSEFLFMNRAGELPGEDEQFEAYRAA
                     VLAMNGLPVTIRTVDVGADKPLDRMSVNELRHEHALNPALGLRAIRWSLSEPAMFRQQ
                     LRAILRASAFGKVKLLVPMLAHVGEAMQTLDAIARAKQQLVDAGKPFVDVEVGAMIEV
                     PAAALVMPSLLKLFDFVSLGTNDLIQYTLAIDRGDESVAHLYDPWHPAVLKLIEGVIH
                     HARLAGKDVSVCGEMAGDTAFTEVLLAMGLRNFSMHPSQISSVKQVVLRCNTRRLAPL
                     VESAFRGDDLWTGCERLRESAMVA"
     gene            complement(20235..21515)
                     /locus_tag="PKV56_06755"
     CDS             complement(20235..21515)
                     /locus_tag="PKV56_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOX67549.1"
                     /translation="MNTTPHPQDEAAPLIDRAIRLAVVGAGPVGLSLAIQAARALPRA
                     QISVFDARAIDADISADPRTLALSLGSVQLLQRLGAWQDGMGQPITEVHVSQQAPSFG
                     GLLGRWMGEPQVRIRAAEMDVPMLGAVLSYGTVTAALQQVWMEACKAGPQRLITRFST
                     PVRSLKPVEPADGASSPGGVEVDADIAETFDLAVIAEGGVFAEQERKPVTRDYGQVAW
                     VGRVTLDGARGGVAYERFTRHGPAALLPLGPDQAALVWCVDAGDDPVAELTDVQRCCV
                     LNTIFPPEAGRITSVSPLKRFQLGLNAERTLTGGVERDGHREPGRVVRIGNAAQTLHP
                     VAGQGLNLGLRDAHVLVRELVRSNDVDQALRRLDRKRAPDRWSTIAATDFLARSFTWE
                     WPGASGARGLGLAALQTLSPLKSALTRQMMFGRR"
     gene            complement(21591..22982)
                     /locus_tag="PKV56_06760"
     CDS             complement(21591..22982)
                     /locus_tag="PKV56_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOX67550.1"
                     /translation="MTHSTHAARRAMLAQRLHSVGIDIAVVPTAPERQRNADSHHPYR
                     HDSHFHYLTGFDEPEAWLVLHSDGHSTLFCRPVDPAREAWEGPRLGVETAPAALGVDE
                     ARPIESLDAELPLMLANRRAVGLPFGRHPELQTQVEAWLTKVRTRERVGVECPTVLHD
                     LHPLIAEMRLHKDAEELTTMRRAATISAGAHRRVMRFCGQRFREGAASLREYELEAEL
                     LHEFRRLGAQSPAYPSIVAAGANACILHHPAGAAELRPDELCLVDAGCELDGYASDIT
                     RTFPASGRFSSPQRELYAVVLAAQQAAIEVTRPGQRQRDAHHAAVRVLTQGMLDLGLL
                     SRDRHGDVDDIVASAAYRQFYMHGTGHWLGRDVHDVGEYLSLEEDPFEQPDGYGGTLV
                     KRPSRVLCPGMVVTLEPGLYVRPAADVPERYWNLGIRIEDDAVVTDSARGCELISRDA
                     PVDPLEIEALMRG"
     gene            23108..24196
                     /gene="dmeF"
                     /locus_tag="PKV56_06765"
     CDS             23108..24196
                     /gene="dmeF"
                     /locus_tag="PKV56_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HOX67551.1"
                     /translation="MKAHTHDMSPWQHSHVFDSGNRRGEARTRVVLAVTLITMVAEIV
                     GGWWTGSMALLADGWHMGTHALALGVAAVAYALARRQALDGRYAFGTWKIEVLGGFAS
                     ALVLALVAAGITLESALRLWRAEPIEAVTALWVAVIGLVVNLVSAWLLHGADDAHGHD
                     HAHGPVDPHDHGHDHHARAEDHAHAHPQAQSAAAPVGGGHDLNLRAAYVHVLADALTS
                     LLAIAALSGALWLGWWWLDPLVGVLGAVVIGVWAAGLMRQTAAVLLDREMDHPLTAQI
                     RAAIESDGDARVADLHLWRVGRQHYAAIVSIVADAPFAPAVYRTRLAEYGALAHLSLE
                     VNRCPRADECDAEPAAESLTTLPLVKRH"
     gene            complement(24207..24920)
                     /locus_tag="PKV56_06770"
     CDS             complement(24207..24920)
                     /locus_tag="PKV56_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX67552.1"
                     /translation="MVLAAGRGERMRPLTDATPKPLLAVHGKPLIEWHLEGLARDGVR
                     DVVVNTAWLEDRIVETLGDGSRFGLTIHYSCEGRDHGGALETAGGIATALPLLRGMAG
                     QLDSDCFWVVSGDIHVPGFRFDAEVGRRFAAGHHDAHLWLVPNPVYHPNGDFGLSADG
                     HALADGPGPDGLRWTYANIALCRAALFREVAPGTRAALGPLLYAAMRERRITAEVYPG
                     AWENVGTPAQLAALNADAA"
     gene            25118..27145
                     /gene="tkt"
                     /locus_tag="PKV56_06775"
     CDS             25118..27145
                     /gene="tkt"
                     /locus_tag="PKV56_06775"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOX67553.1"
                     /translation="MAAAASDQNTSLMANAIRALAMDAVQQANSGHPGAPMGMADIAV
                     ALWGRHLKHNPGNPHWADRDRFVLSNGHGSMLIYALLHLTGYDLPMSELRNFRQLHSK
                     TPGHPEVGVTPGVETTTGPLGQGITNAVGMALAEKLLAAEFNRPGHTIVDHHTYAFLG
                     DGCLMEGISHEACALAGAWRLNKLIALYDDNGISIDGQVKPWFIDDTPKRFEAYGWNV
                     IANVNGHDVDAVDAAIVQAKSAGKPTLICCKTTIGKGSPNRGGTAKAHGEALGAEEIK
                     LTREALGWTSEPFVIPETVYEQWDARHSGLAAESKWVDAFEAYGKAHPELALEYVRRV
                     EGHLPKNFAQVAVDAALAAHTKAETVASRKASQIALEAFTKAMPELLGGSADLTGSVF
                     TNTSSTPALRFGDDGSSNGGRHINYGVREFGMAAIMNGVALHGGFIPYGGTFLTFSDY
                     SRNAVRMAALMKLRVVHVFTHDSIGLGEDGPTHQSIEHVASLRLIPGLDVWRPADTAE
                     TAIAWACALESRQRPTALILSRHNLPYAPKSGLDDISKGAYVLAEPSEVGLKKKAQAV
                     IIATGSEVSLALHAQQLLAQQKVAVRVVSMPSTSVFDRQSVGYKTSVLPAGLPRIAVE
                     MGVSDGWWKYGCAAVVGIDTYGESAPAPVLFKHFGFTAENVVATVLRAISR"
     gene            27142..27513
                     /locus_tag="PKV56_06780"
     CDS             27142..27513
                     /locus_tag="PKV56_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67554.1"
                     /translation="MKAAWLSLAVVLTGVSAVAEVCAQTILQIRVVPAKPKAGEPATL
                     AGRFGPGAAAPNCNVRVDFGDETPTQDVLIKDATELPLMLAHVYAQGGDYVVSVQPVT
                     KGKVPACSGGNHSTLIEVQAP"
     gene            27569..>28278
                     /locus_tag="PKV56_06785"
     CDS             27569..>28278
                     /locus_tag="PKV56_06785"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOX67555.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVQNFAEIEIVGINDLLEPDYLAY
                     MLQYDSVHGRFKGTVSVEGSTLIVNGRKIRLTAVKDPAELKWNEIGADIVVEATGLFL
                     TKETGAKHLAAGAKKVIFSAPSKDDTPMFVYGVNHGAYKGEAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTS"
BASE COUNT         4698 a         9219 c         9419 g         4942 t
ORIGIN      
        1 atcgaacgcg cggccgggcc ctcgccgatc atgatgtcga tctggtagtc ctccagcgcc
       61 ggatagagga tttcctcgac gaccggactc aggcggtgga tctcgtcgat gaagagcaca
      121 tcgttgcgct ccaggttggt caggatggcc gccaggtcct tcggcttctc gagcaccggc
      181 ccggaggtct ggcgcaggtt cacgccgagt tcattggcga tgatgtggct gagcgtcgtc
      241 ttgcccagcc ctggcgggcc gaacagcagc acgtggtcca tcgcctcgct gcgcatcttg
      301 gcagcaccga tgaagatctc gagctgctcg cgagccttgg cctggcccac gtactcggtc
      361 agtcccttcg gtcgcagcgc gcgctcgatc gcctcttcgt tgggcgaagc gggcgccgcg
      421 ctgaccacgc gccggggctc gaattcgtcg gtctggatgc tcatgccgca ttatcgacat
      481 ggccctgcgg cggccggtgc cgagagaccg gaagcgtcac cacgtcacct aaacttcgtc
      541 gatgaatttt ttcaggcacc gctgccgctg atcctgcgca tcgcgttgat cgcgctgccg
      601 ggcgtggcgc cggtgccgtc gctccctggc gcaccccggc cctcgcggat tccagatcct
      661 gatccgatgc catcggccag gcgctgatgt gcggacgatg cagttcggac cctgtgtcga
      721 atgcgaggcc cgactgtccg cgactctcga ggtgcaccac ggcctccctc atccaacgct
      781 cctgactctt gcgagaacga atccatgaac aagctttcca tccagcgatt gatcgccgtc
      841 actgcgctgt cctttgccgc cctgggcgtc acggcccaca gcttcaaggt cggcaatatc
      901 gacatcggcc acccgtatgc gcgacccacc agttcgggcc agcaggtcgg tgccggctac
      961 ctcaaggtca ccaaccgtgg tgccgccgat cgcctgcttt cagccaccgc gccggtggct
     1021 gcctcggtcg agatgcattc gatggtgatg gaaggcgacg tgatgaagat gcggcaggtc
     1081 gacgcgatcg acctgccggc cggccagacg gtcgagctga agcccggcgg ctaccacctg
     1141 atgctgatgg ggctgaaggc gccgttgaag gatggtgaca agctcccgct gacgctgaag
     1201 ttcgagaagg ccggcgtggt ggtggtgacg gtgaacgtcg agagccccaa gccgagcgaa
     1261 gctgcggccg agcacaagca ctgatcggcg ggacatcccg ccagcggatg cgctgtcaac
     1321 cgagcgattt gagcgccagc ttgatgccgt cgctgacgct ggttcctgcc ggcaggttct
     1381 tcagcgcagc agcggcctcg cggtcgctgt agccgagcgc gatcagcgct tgcaggatgt
     1441 cggcctgcgc gtcgtcggcg atcgatgccg gggcgctgat ggcgtctgcg agcttgccct
     1501 tgagctcgag cagcaatcgc tcggcggtct tctttccaat gccgggcacc ttcacgaggc
     1561 gtgcggtttc ctgtcgtgcg acagcctggg cgagctcgcc gacgctcagg cccgacagca
     1621 gcgacagcgc catccggggc ccgacgccgg tgatccggat cagctggcga aaggtctgtc
     1681 gctcctcgtg cgtgaggaaa ccgaacagga tctgcgcgtc ctcgcgcacg acgaaatgcg
     1741 tcagcagcgt gacgcgttcg ccgagcccgg gcaggttgta gaagctgctc atcggcacgt
     1801 cgacttcata gccgacgccg ctcacatcga tgagcagttg cgggggcgat ttttccgcga
     1861 gggtgccggt gagtcgtccg atcatcggcc gattatccgg tcgccctcgg gcggcagcgg
     1921 atcagctgcg gaacagtccc agccgctgag cttccagcgc agcttccgcc cgcgaactca
     1981 cattgagctt gcggtagatc tgtttgacat agtccgcgat cgtgtgccga gacaggctga
     2041 gctgcacgcc gatctcgggc agcgtgtagc ccttggccac gcgcagcagc acttcgttct
     2101 cgcggtcggt cagcgagaca tgaggcatgg cgtgcgcctg ttgcggcggc ttgcttctcg
     2161 attgcgcggc gaagtgggag atcacgcgcc gtgcgatcga tggcgacagc ggtggctcgc
     2221 cgccgctcat gcgcttgagc tgctcggcga tcagctcgcg cgactgctcc ttcagcaggt
     2281 atccgaatgc gccggcttgc aacgccggga agaggtggtc gtcatcgtcg tggatggtga
     2341 ccacgaccga ctgcgcctcg ggttggttct cgcgcagttc ggccaccacc gtgacacccg
     2401 atccatccgg caggcccaga tcgaccagcg ccaggtcgaa cttgatcgct gtcgtcagtt
     2461 ccagggcgtc atggatgcgc gcggcttcat ggatgtgcga tttcggaaac acctgcagca
     2521 ccagcgcctt catccaggcg cgaatctcgg gcaggtcttc cagcagcagg atgttgttca
     2581 tcgggactcc gtcggtcggg ttatcgacca aaacggcgcc gtcatgatcc cgatcggaca
     2641 tggacatcga tgatttacag gggaatcgtg agcctgatca cggtgccgta gcccggtcct
     2701 gactcgatca ggcactgacc gagcaactgc ttggcgcggt gcttcatgct ggacatgccg
     2761 tggccccgat cgagcttgcc gtcgagttcc atcgcgattc ccttgccgtt gtcctgcacc
     2821 agcagcccga aatgctgtgc gtcgacggag gtgcggacct tgcaatgcga cgcgccgctg
     2881 tgcttgatga tgttgctgac cgcctcgcgc aggatccggg tggtctgcac gaacacgcgc
     2941 gcggtgagtt gctgttcaag ttcgtccacc gggttgcgcc agtcggcttc gatgttggcc
     3001 tgacccagac gcgacacggt ctcggcgcgc cagtcggcca gcgcgtcggc cagccgcacc
     3061 ggcttgccgg tcagcccgcg caccgacagg cgcatctcct ccagggcctc gcgcgccagc
     3121 gtggagatgc gttcgctctc gctcgtgtgc acgatggtca gcagcttggc cccgaggtcg
     3181 tcgtgcaagt cggcggcgat gcgtttgcgc tccttctcgg tcacctgctc gacgcgcagc
     3241 gccgacagtt gcgcgaagtt gcgctcgatc tccgcggtgg cttcctgcac gcgcttctcc
     3301 agcagcgtgc gattgacttc tgccgcctgc agcgcgcgcc cgaacacctg cagcagccgc
     3361 gatccgatgc ccagcagcag cagtggaaac gcgaagttca ggggctgcac ggccagccgg
     3421 ggcaaccagc cgtactgcat cgcgatttcg ttcatcacga gcaggcccat gcagcccagc
     3481 gtgattccca tcggccagaa atcgtcgcga tggttcttcc acgcaagcca caggtagacc
     3541 gcgatggctg cgatcagttc ggctgccatc agcatgaacc aggcgctggc cgacgtgaag
     3601 aggtggtctg caccgaccgc ggcgaggctg atcggcacca gcatgcactg cagcagcagt
     3661 gcgatcgcga ccgggcgcga tcgcactccg gcatagctca gcaggaactg caccgccatc
     3721 gccaccacgg gcggaaatgc gctgcacacg acgatctcga acgtgtgcgt ctcccagggc
     3781 cactgcggcc accacacgcg cagagacagc acggtccatc cgatcagcag ccacccgaag
     3841 tacatgagga agatctcgct gcggttgatc cagctcagga tcagcaggaa acccccgagc
     3901 gcgatcagcg tgatgccgat gatctggacg gtccggatgc tccagaactc gcgctgttcg
     3961 tacaacgcct tcatctcgga tgcgggcccg atccgcagca ccgacagacc gcctgcgcgc
     4021 gcgcgagccg agacatgtct cagcgccgag ccgtgtacct ggatgtcgag cacgttcagc
     4081 ggctgatcga gcagaaccgc cggcaggttg accaattgcg ggcgatggca gttgcgcgcc
     4141 gtcggctcgg ccatgcgccc gccgctgaag attcgtcggc cgttcaggtg cacttcgagg
     4201 ttgctgcagg cgcgctcgat gtagagcgcc agcaggtcgt cgggcgccag accggccggc
     4261 ctgacgaagc tggcccggta ccacacgctg ccgtcgtggt cggggcgaga tgcggcccag
     4321 tcatcgggca gccgaactgg cgcgggcgtc ccctgggtcg ggaagtgcgc ctcgttgctg
     4381 ttgatcgaga gcgccgaatg gatcgtcagc gcttcggcgt gggccgcacc ggtgaccagc
     4441 cacgcccaga gggcgaggat caggaacagg gcacgggtcg gcatgggcgg cgattgtgca
     4501 ggatgctctc gggcgttgaa acgcctgcac cgggggcgct gcaggtgccg cgccacaata
     4561 ggagaacccc tgcacagatc ggtcgaactt gatcctcaga cacgccttca ttcctctgga
     4621 caatgcccgg ctggcacatc tgtgcggcag cctggactgc catctgcgtg atatcgaatc
     4681 ggcgttcgac gtgacgatca cgcggcgcaa cgagtcgttc cgcattgaag ggggaaaggc
     4741 gcaggcagag cgtgcagtgg cgttgctgca gtcgctctac gaccgcgccc agcgcccgct
     4801 ggcggacgat gcctttcagc tgtcgctggt ggaagccgcc tacgacagcg cgggccgcgc
     4861 gattccgcag gcgccgctcg ctgccgcggg cgggcccgcg ccggaagtcg tgcccgccga
     4921 aagccggtcc gctctgcgca cccgccgtgc cgatctggcc gggcgcacac ccaaccagca
     4981 tctgtacctg gccaacatcc agtcgcatga catcaccttt ggcatcggtc cggccggcac
     5041 cggcaagacg tttctggccg tggcctgcgc gatcgacgcg ctcgaacgca gcagtgtcca
     5101 gcgcatcgtc ctgacccgtc cggcggtcga ggccggcgag cggctgggtt tcctgcccgg
     5161 cgatctggcg cagaaggtcg acccgtacct gcgcccgctc tacgatgcgc tttacgacct
     5221 gatgggcacc gacaaggtga ctcgcgcctt cgaaaaggga atcatcgaag tggcaccgct
     5281 cgccttcatg cgcgggcgca cgctcaacaa cgcgttcgtg atcctcgacg aggcacagaa
     5341 cacgacaccc gagcagatga agatgttcct gactcgcatc ggcttcggca gcaagtgcgt
     5401 ggtcacaggc gacgtcagcc agatcgacct gccgcgcggc cagatgagcg gtctcatcga
     5461 cgccgagcgt gtgctcaggc gggtgcgcgg ggtcgcgacg acccgcttca gctcggccga
     5521 tgtggtgcgc cacccgctgg tcgcgcgcat cgtcgatgcc tacgaggcat cgcgcgcgga
     5581 cactgtctga gaccgtgagt cgtccgacct tggccctgtc tctgcagttc gcgcgcgagg
     5641 acctgccccg cgcgcaccgc tgccatctgc cgcgccatcg ggtggcgcgc tggatacgtg
     5701 ccgcgctgga cgcgccggcc gacatcaccg tgcgcatcgt cgggagcgag gaagcgcggg
     5761 cgctgaaccg cgaattccgc ggcaaggact acgcgaccaa cgtgctgacc ttcgactaca
     5821 gccatgcgcc gcaggtgcat gccgatctgg tgctgtgcgc cgacgtggtg gaggctgaag
     5881 ccagggcgca gtcgctgccc gtcgaggccc attacgccca cctgctggtg cacggcgcgc
     5941 tgcatgctca aggccacgac cacgaaagcg acgacgatgc cgagcgcatg gagtcgcgtg
     6001 aaagcgccgt gatggtcgcc ctcggattcg ctgatcccta cgccgactga cgtgcggaac
     6061 gtgctatcgg ctcagtgctt cgagccgagg taacgcttgc gccagaagaa cacgctcagg
     6121 ccgatgccga gcaccagcat cgccatgaag acggcccaga agccggacat gctgtggatc
     6181 agcggcaggc cctcgaagtt catgccgaag aaccccgtca ccaggttcag cggcatgaag
     6241 atggcggtca gcacggtcag ggtgcgcatg atgtcgttgg tgcggttgct ctgcgccgag
     6301 aagtgcatct gcactgcggt ttcagccgac gactcgagtc gtcgaacatg gctcagcacc
     6361 cgctcgatgt gttccagcac gtcgcgcgag cgcacgcgca gcaactcgcg ctcacgccgt
     6421 gcgttcttgt cggcgtccgt cggccactcg tcgagcgcat cgatccattc ggtgatcgcg
     6481 ctgcgctggt cctcgcaggt gtcctccagc aggtgcagcg cgttgcgtga ctccagcagc
     6541 atctgccagt tgtcgaaacg gctgcgcggg ttgaacagtt cctgctgcag gtaggtcagc
     6601 tgcttggtca gcagccgtcg cagttcgagg tagctgtcga ccatgtgatt gaccatgcgc
     6661 agcatcaggt cggcggcgct ggcgggcagg cgcgcagcgt tgcgaccttc gagcatgcgt
     6721 gcgtcacttc ctgcgggcag cgcctgctgc tgcaggcggt tggcaaagaa ctcgcgcacc
     6781 tggcaatcgg tcggatgcac tgtcagcagc acgcggtcga acagcgcgaa tccgaccggg
     6841 ctggtgtcga tggcagccag cgcagcccgg gcggtggcca gggtgccctt gctgtcgtcc
     6901 aggaacatcg gcgcgctgcc ttcgccggcg gccagtcgcc ggaacacgag cacgtcgtac
     6961 caggaggtgt agtcgaagtg cgacggcagc tggttgttca gcagatcgga gatgtgcagg
     7021 tcgaccagct gtccgccggt ccagccctgc agtgcggcct gcagcaccga ggattgcact
     7081 tcgaactcgc gccgcgcgct gcccacccac aggtagcccg ccgggggcaa tttctccggc
     7141 agcgtctcga gttcggtgaa ctggtcgccc tggatgtgga agacgcgcat cgccggcccg
     7201 gctccggttt caaccggcct gcatcaggcg cgccgcgtcg agtgcgaagt acgtcagcac
     7261 accatcggcg cccgcccgct tgaatgccag cagactctcc atcatcaccg cgtcgtggtc
     7321 gagccagccg ttggcagagg ccgccttcag catcgcgtat tcgccactga cctggtaggc
     7381 gaaggtcggc acgtggaact cgtccttcac gcggcgcacg atgtcgaggt agggcatgcc
     7441 cggcttgacc atcaccatgt cggcgccttc ggcgatgtcg agggcgacct cgcgcagcgc
     7501 ctcgtcggtg ttcgcggggt ccatctggta gaccttcttg ttgctcttgc cgaggttgcc
     7561 tgccgagccg actgcatcgc ggaacggacc atagaacgca ctcgcgtact tggcgctgta
     7621 ggccatgatc ttggtgtgga cgtggccgcc ggattcgagc gcctgccgga tcacgccgat
     7681 gcgcccgtcc atcatgtcgc tcggcgcgac gatgtccacg cccgcctcgg catgcaccag
     7741 ggcctgctgc cgcaacacgt ccaccgtggg gtcgttgacg atatagccgg cggcgtcgag
     7801 cagtccgtcc tggccgtggc tggtgtacgg atcgagcgcc acatcggtga tcacgcccag
     7861 ttcggggaac cggcgcttca gctcgcgcac caccctcggc accaggcctg ccgggttcag
     7921 cgcctccctg ccatcctcgg tcttcagcgc cgtctcgatc accggaaaca gcgcgatcgc
     7981 cggaatgccc aggttcacgc agtcctcggc caccaacagc aggcgatcca gcgtgagacg
     8041 ccggactcca ggcatcgaag cgacatcctg cgcgcggtcg tggccctcgg tgacgaagac
     8101 cggcaggatc agatcgctcg gatggagacg gtgctcgcgc accatcgcac ggctgaactc
     8161 gtctttacgc aggcgccgcg ggcggcttgt ggggtacgca ggaggtgtgt tcacgacgaa
     8221 acgcagagat gtgtggacag gaatcgacgt gtgcgttcgg tgcccgacaa caggcacttt
     8281 ccgcccacga acgaggtgcg gcggatcata gacgcacgct gtcgggcgtg tacaccgatg
     8341 ctgcagcgcg tgatgactgg atagttcccg cgtggcgata atccgtcgat gctctggatc
     8401 aaagccttcc acatcgtgtt tgtctcggcc tggttcgcgg gcctgttcta tctgccacgg
     8461 atcttcgtga atctggcgat ggtgccggcc gacagccatg ccgagcggga acggctgctg
     8521 ctgatggcgc gcaagctcta ccggttcacc agcctgctga tggttccggc tctggggctg
     8581 ggaatctggc tgtggctcgc ctactggaga acaggcgctg gcggctggct gcacgccaag
     8641 atcgcgctgg tggtcctggt ggtcggttat caccacatgt gccgcgggct gttgcggcag
     8701 ttcgagcagt tcgccaacca gcgcagcgat cgctggttcc gcgtgttcaa cgagttgccg
     8761 gtgatcctgc tgatcgtcat cgtggtgctg gtggtggtca agccgttttg accgcaggct
     8821 gacgtcagcg ttgccggagc accgatggcg cgacaccgaa gttcggcctg gctgctggca
     8881 tggttgtatg cgggtctgat cgcctacgcg agcctgtatc cgtttgccgg ttggcgatgg
     8941 ccgacggtct ggcccctggc gttcctgacg ctgccttggc cgcggtactg gacgggcttc
     9001 gatctggtat cgaatctcct gggatacctg ccgctcggtt tgctggtgtt tgcggcccag
     9061 gtgcgcacgg ggcgaaacgc cggaggcgct tttctgcgcg ccgtactgag tgctgctgcg
     9121 gcgtcgctcc tgatggaaat ggtgcagaac ggcctgccgt cgcgcgtgcc gtcgaatgtc
     9181 gatctggctt tcaacacgct gggcgccgca gcgggcgccg tgctgggcgc gctgatgcac
     9241 gtggtcggtc tgctcgaccg ctggcagcgc ctgcgcgacc ggtggttcgt gccgcaaagt
     9301 gccggcggac tgaccctgct ggtgctgtgg ccgatcggcc tgctgttccc gagcgcgctg
     9361 ccgttcggcg tgggtcaggt gctgtcgcgg ctccaggaca cgcttgctgg ctggctggat
     9421 caaacgccgc ttgccgaatg gagccgcaac atcctggagg cacagtggtt cggtccggga
     9481 ctgcctccgg ccggcgagtt ttgcgccatc gcactggggc tgatcgcacc cagcctgctc
     9541 gcgttcacgg tgtgccgctc gggatggcgg cgcagcgtga tcgcattggg tgtggcgggt
     9601 gcgggcgtgg gcgcgacgac tttgtcgacg gcgctcaatt tcggccccga ccacgccacg
     9661 gcctggcttt cctccacggc cgtggcgggg ctcggcgcgg gtgccctggc ttcgctgctg
     9721 ttggcctggg tgccgcgcag ggcctcggct gcactgggcc tgatggcgtt gaccgctctg
     9781 gtgtcgctga tctcccaggc atccgaagac gtttactttg cccagagcct gcaggcctgg
     9841 gagcaagggc gtttcatccg attccacggc gccgcgcagt gggtcgggtg gctgtggccc
     9901 tacgcggcaa tggcctatct gctgggtcgc atcgctgcgc gtgacgagga ctgacgggcg
     9961 cgccacgcgt gcttcccctg cggcggctgc atttcgatac ctgacggacg gctggcgacg
    10021 cgggtggctt cgtagaatgc ggaatgagct atttcaatcg gcatgttttc ttctgcttga
    10081 accagcgcga tgcgggcgag gagtcctgcg ccagacaggg cgcacaggcc ggcttcgatc
    10141 gttgcaagtc gcgcgtcaag gaggctggct tgtcggggcc gggtcaggtc cgggtgaaca
    10201 aggcaggttg cctcgaccgc tgttccggcg ggccggttct ggtggtttat ccggaagccg
    10261 tctggtacac gtttgtcgac aacgacgaca tcgacgagat cgtcgattcc cacctgaaga
    10321 acgggcaggt ggtcgaacgc ttgctgatcc cgccctccgt tggcaagtga cagccagcct
    10381 ggcgcgggcg ccgtcgtcgg gcgctccatg aactcacaga cccagtccgc acaggtcgat
    10441 ggcccggctg gcagcctgca gtgcgcgctc gacatgccgg ccacgccggc catcggtgtg
    10501 gcggtggtat gccatcccca tccgcaattc ggcggcacga tggacaacaa ggtggtgcag
    10561 acgctcgctc gcgcgctgct gcaactgggc tgggccacgg tgcggttcaa ctttcgcggc
    10621 gtcggaaaat ccgcaggcgc gtgggacgag ggccgaggcg aggtcgacga tgcgctggca
    10681 gtcatcgaac gacatcggat cgcgggtcag ccgctgctgc ttgccgggtt ctccttcggg
    10741 gcctacgtgg cggctgaggc cgcctcgcgt ctgcctgaag cgcagcgtcc gctgcggctg
    10801 gttctggtcg gcccgtcgac gcaaaagcag tgcatccctg ccgtgcctgc cgacacagtg
    10861 gtcatccacg gggagagcga cgaagtggtt ccgctctccg cgacgctgga ctgggcgcgg
    10921 ccgcagtccc tgcccgtgat cgtgctcccc ggcgtcggcc acttctttca cgggcagctc
    10981 ggttggctga agaccgtcgt ggtgcagcaa ctgcagctgg cgcctcgatg agctgagtcc
    11041 gtccagcccg ccgctcccgt tcctattctt tcctctggtt gatccatcga tgaatttttc
    11101 cctccgttcc ctgtcccgtt cgttctccgc cgggctgctc ttgatcgctg gcgtgttcgc
    11161 gctctcgacg gcgcaggctc agatgcccca gcctcccgag gtcgcggcac gcagctacct
    11221 gctggtcgac ctgaccagca accagacgct ggcggagcgc gatgccgatg cgccctccga
    11281 tccggcctcg ctgaccaagc tgatgacggc ctacgtggtc ttcgccgcgc tgcgcgacaa
    11341 gaagctggcg ctcgaccaga agttgtcgat atcgatgcga gcctgggaag agcgcaaggg
    11401 cggcggctcg ctgatgttca tagacaccac gatgacgccg accgtggacg aactgctccg
    11461 gggcatgatc gtccagtctg gcaatgacgc ctcggtggcg ctggccgagg cggtcggtgg
    11521 cacgctcgac cagttcgtcg cgatgatgaa ccggcagtcg caggcctggg gcctgaagaa
    11581 caccgcgttc aagaatgtca ccggcctcac cgagcccggc cacaggagca cggcacgaga
    11641 cctgtcggtc attgcttcgc acatcatccg cgacttcccg aaggagtacg cgtactactc
    11701 catgaaggag ttccgctaca acaacatcac gcagcccaac cgcaacctgc tgctgcgacg
    11761 tgatccgagc gtcgacggca tgaagaccgg ctacaccgat gcggcgggct actgcctgat
    11821 cgccagcgcg cagcgcgagt ttccgaacct cggcgccggc ggcaacggag gcggtccgcg
    11881 tcgcctgctc agcgtggtgc tcaacaccgt gtcgatggag gctcgagcca acgaaagcca
    11941 gaaattgctc aactgggggt atcaggcttt cgagaccgtg cgcctgttcg aggtgaacaa
    12001 ggcggtggcg acggtgccgg tctggaaggg acagaccgac caggcgcaac tcggcgccaa
    12061 tggcgcgctg ttcgtgacca tgcccagggg agaaggcggc aagctgcaga ccaagatcga
    12121 gcgcaccgac ccgctggtcg cgccgttgct tcagggtcag cgcgtgggca ccatcaaggt
    12181 gtcgaccgct gcgggcacgg cggtcaccga ggtcccgctc gtggtgctgc agcccgtgga
    12241 agccgccggg gttttcggcc gggcgtggga cgcgctccgg ctttggatca agtaacctgc
    12301 ggatgtcgcg cgacggcatc ttgaggacct gaaatgccca accttcccga cacgctttgc
    12361 tacctcgatg gcaactacac cccgctgaac caggcgaagg tgtcggtgct cgaccgcggg
    12421 ttcctgttcg gtgatggtgt ctacgaagtg gtgccggtct acgggcgggg tctgttccgt
    12481 ttcgaagagc acatggcgcg tctcgagcgc agcctggcca aggtgcgtat cgccaatccg
    12541 catacggctg cggagtggct ggctcgctgc cgccgactga tcgagtccca cgtcgccgag
    12601 gatcagttga tctacatcca ggtgacgcga ggagtggcgc cgcgcgacca cgtgatgccg
    12661 accgacatcg aaccgacggt cttcatcatg gtcagtgcgt tgaagcaacc cagcgccgag
    12721 cagcgccacc gcggcgtcgc ctgcgtgact gcgcgcgatt tccgctggga gcgcggcgac
    12781 atcaagagca tctcgctgct gggcaacgtg ctggcgcggc agatctcggc cgaccacggt
    12841 gccgtcgaga ccatcctgtt ccgcgacgga ttcctcaccg aggcggcatc gtgcaacgtg
    12901 tgggtggtgc atgaaggtgc cgtgctgggt ccccccgcga gcgagcatgt gctcgagggc
    12961 gtgcgatacg agctgatccg tgaactctgc gatgacgtcg gcatcgcctt caacctgcgg
    13021 ccgatcccgg aaggtgaggt ggttgcagct gacgagctga tgctcagttc ggccagcaag
    13081 gaggtgctgc ctgtgacgat gctcgacggc gaatcggtgg gacatggcgt cggacgcggc
    13141 aagcccggcc cggtgtatgc gaagctctac gcggcctatc agcgtgccaa ggctgcgcag
    13201 cggctgtgac gatcccgacc atgcaggaca tcccgcgcga gccatcgctg atcgagtacc
    13261 ccgcgcagtt tccgatcaag gtgatgggtg gcaatgtcga tggtttcacc gaggcggtcc
    13321 tggaggtcgt gcggcaattc gatccggcct tcgacccgca gtccatcgag aagcgctcca
    13381 gctccggcag caagtatctg ggcctcaccg tgacggtcac cgcgaccagc cgcgtgcaac
    13441 tcgacgacct ctaccgagcg ttgagctcgc atcccctggt caaggtggtt ctgtagggcc
    13501 gcacgcaatc gatgactgca cccacactgg cctgtctggg cctggtcgac gccgccgaga
    13561 cgatcgaggc gatgcgcgcc tgcgtcgagt tacggaacgc tgcacttctg ccgacgcccg
    13621 atgagatctg gctgtgcgaa cacctgccgg tgtacacgca ggggcttgct ggcaagcccg
    13681 agcacctgct tgatccgggc gacattccgg tgatccagac caaccgcggc gggcaggtga
    13741 cgtatcacgg ccccggccag gtcgtggcct accccctgat cgacttgaag cgcctgggca
    13801 tctacgtcaa ggagtatgtc tatcgcatcg agcattgcgt gatcaagaca ctcgaaagct
    13861 acggtgtcac tggccaccgg gtgcctggcg caccggggat ctatgtccgg ctcgaggatc
    13921 ccggcggtca cgctgcgttg gagtccgtgc cgccgggcga gcgtcggggc gtcgctgctc
    13981 cggacccgtt ccgcggtctc ggaaagatcg ccgcgctggg catcaaggtg agtcgtcatc
    14041 acgcctacca cggtgtcgcg ctgaacgtgg cgatggacct gagtccctac cagcgcatca
    14101 acccctgcgg ttacccgggg ttggcgacgg tcgatctggc tacactgggt gtgcacgccg
    14161 acgtagcgtc ggtgtcgcgt cagttgggcg cccggttggc cgagcatttc accccctgac
    14221 cccaccggca gcctgcgctg cgagcagccc cctccctttg aaagtccgcg atgtccagcg
    14281 attccgtgag ccctgttgcc tccgatgcgt ccgcatacga cgccagcgcc aagcagaaat
    14341 cacaggcgaa gacgtcgcgc atcccgatca agatcgtgcc ggcggaagcg ctgaagaagc
    14401 ccgactggat ccgggtcaag gccggttcac ccagcacccg gttctacgag atcaagcaga
    14461 tcctgcgcga gcagcagctg cacaccgtct gcgaagaggc ttcttgtccg aacatcggtg
    14521 aatgcttcgg caaaggcaca gcgaccttca tgatcatggg cgacaagtgc acccgtcggt
    14581 gtccgttctg tgacgtcggc cacgggcgtc ccgacccgtt ggacgccaac gagccgctca
    14641 acctcgcgag aacgatcgcg gcgctcaagt tgcagtacgt cgtgatcacc agcgtcgacc
    14701 gcgacgatct ccgtgatggc ggtgcggggc acttcgtcga ctgcattcgc cagacgcgag
    14761 ccttgtcgcc tgcgacgcgc atcgaggtgc tggtgcccga tttccgcggc cgcgacgatc
    14821 gtgcgctgga aatcctgaag gccgcgccgc ccgatgtcat gaaccacaac ctggaaaccg
    14881 caccacggct gtacaaggag gcgcggccgg gcagcgacta cgcattcagc ctgaacttgc
    14941 tgaagaagtt caaggcgatg caccccgagg ttccgaccaa gagcggcgtg atggtgggcc
    15001 tcggcgagac cgacgaggaa attctgcagg tgatgcgcga catgcgcgag catgacatcg
    15061 acatgctgac catcggccag tacctggcac cgagcggtca ccacctgccg gtgcgccggt
    15121 acgtccaccc ggacaccttc aagatgttcg aggtcgaagc cctgaagatg ggattcaccc
    15181 atgctgccgt cggtgcgatg gtgcgcagca gctaccacgc cgaccagcag gcccatgctg
    15241 cgggtgtcgc cgacgcgctc ccgcgctgag cgtcagatca actgtcagga gtcatccatg
    15301 caacgcagaa gtttcaccag cggtctgttg tcgggcgtca gcctttgcag cgttggaatc
    15361 gccccggcgg cccacgccgc ttcggtgctg agcgatgccg atgcctcgtc cggcatccgc
    15421 gccgcgctcg agcgcggcgc cgaagccgct gtttcgctgc tcggcaagcc cgatggattc
    15481 ctgggcaatc caaaagtcaa gattgcgttg cccggcttcc tgaaggatgg cgccaagctg
    15541 ctgaaggcca cgggccaggg caagcgtgtc gatgaactgg tgaccgcgat gaatcgcgcc
    15601 gccgaggccg ccgtgcccga agccaaggaa ctgctgctcg gcgcggtgaa gtcgatgagc
    15661 gtcgaggacg gcgctcgcat cctgtccggc ggcgacaact cggtgacgga tttcttcgcc
    15721 cagaagacgc gcgagccact gggagtcaag ttcctgccca tcgtgaccca ggccaccgag
    15781 aaggtgtcgc tggccgaaaa gtacaacgcg gtggctggca aggtggcggg cctgggtttg
    15841 gtcgacaaga aggacgccaa catccagcaa tacgtcaccg gcaaggccct cgacggcctt
    15901 tacctgatga tcgccgagga agagcgcaag atccgcaagg acccgctggg caccggcagc
    15961 gacatcctga agaaggtctt cggctcgctc aagtaggggc aagggccgcc gggatcggcc
    16021 ggtggcgacg ggacgggcga agctacaggg tcttcgagag ttcgaacatc agcaccgacg
    16081 gcagagctgc ctcgatcgcg tcctgaccga cgcgcgcccg ttcgccgcgt tcggcatccg
    16141 agacattgaa ttcgcggcct tgctcgatga tgtcgatgaa ggggaatcca gggacctcgg
    16201 cgcggatctc cgcgcagaac cgttcgcagc ggtcgccacg caggatgccc tcgatgatga
    16261 tcgcgtgagg caggccgtcc ctgcagaagt cggccgcctc gtcgatcgaa ccgaccatgt
    16321 cgacgagaag ccccatgtcg cgcagagcgt cgcgaacacg cgctcgcact tcgcggcgcg
    16381 aggccacgac gacgacatgg ctgcccgcca gcggcttcga gttcatcgtg ctgtcgaatc
    16441 cctcgtccag gtcgaccatc gtgatgccgt cgatctcgga tcttgccgct gcggtgaact
    16501 cgatctgcac cgtcactgcg ccgtctgcca cctgacgaga taaccccagg ttcatcgtgg
    16561 ccgcggtctg ctcgagcagt cgccaggtca gcgaatcgag cgcgggctgc gccggagcgt
    16621 cggcgccatc ggcggtttcc tgcgcagaag gcaactcgaa tcggcagttc agcctggccc
    16681 gggacttcca gctttgaagc tcgatcgtga actcgatgcg agagcgcgcg tgaaccatgg
    16741 cccagtcgag caacgtgttc agcaggctga acgccaggga ggcatcaacg atcacatcca
    16801 ccggattcag gaccggctgc agttcgatgc ctcgggcttc ggtttcgcgc ttgcggtggg
    16861 tcagcaccgt ctgcagtgaa tcggccagtg ccacgcgttc ccgggactgc tgcatgcgcg
    16921 cattggcaaa cctagccagc aactgggccg tcatgccgac ctggcgagcc gcttcgatct
    16981 cgtcgcgcag tgagcgcaga ccgctgcgat cgatccggcc gctcgatatc agcgcctgga
    17041 tgcgctccag cgcagccgtc agcggatggg cgatttcggc acccagctcc gcgatcacct
    17101 gccgccagcg ctcttcgccc tggcctgcag ggatcggcag tccagatgca gaagcggatt
    17161 tgccgacggt ttcaatggag cccatgtggt ttctgcctgt tcgatgcctg caacggcccg
    17221 gtttgtcgcc ggcgtcgctg cctttctcga aagcagcgag cgaagcactc tgcccgatgc
    17281 ttgctgggtg gtcgaacggg gtgcgagcac gaagtgttcc cgagcgatgc gcaccacgcc
    17341 agagtacttt cctgccgggg ccctggaagc agggggtgaa aaagtcacac cccagccacc
    17401 cggctcgcgc gaggctgacc gcggctcctg cggccggatc gcgcgcaagg accgatgccc
    17461 tcttcgaagc gatgcaattg cattgcctgc cgtgatcgtg gtgggctcgc gagtggcgat
    17521 cggtgcccgc acgtcccggg taccgccccg cgccgagcgc catatgtcaa cccggcgcgt
    17581 cccttccagg tctgtactgt ggttttcgca ccgtcccggt cggcaagacc gctcgctgga
    17641 gcgttgatgg atttccgaag catcgataat ggaatgccat gcccggactg ttgatcattg
    17701 cgcacgctcc gctggcttct gcgttgaagt cggtggctgg gcacacgttt ccggattgcg
    17761 ccgcgccact cgaggtgctc gatgtgccgc ccgacatgcc tctggagcag atcgagcatc
    17821 tggcgcgtga actgctggcc cgtgtccgca acccggaggc gctgatcctc accgacgtgt
    17881 tcggcgcaac cccctgcaac gtggcgcagc gcctggcggg tctggcggaa cccggacagg
    17941 tgaaggtcgt caccggcgtc aacgtgccca tgctctggcg ttcgctgtgc tactgccatg
    18001 aatcacttga tgcggtcgtg gcgcgcgcgg tcgccggtgc gacccagggc gtcatgcagg
    18061 tgtccatctc caggccgcag aaccagacct acaagcccgg ctcacatgat ccaaaccagc
    18121 atcaccatca gcaataagct cggcctgcac gcgcgagcgt ctgccaagct caccaaactg
    18181 gccggcggat tccgcagcga catcttcatc agccgtggcg atcgtcgggt caacgcgaaa
    18241 agcatcatgg gcgtgatgat gctggcggcc ggaatgggta ccgaggtcca gatcgatgct
    18301 tcgggtgacg acgagcaggc ggccgtcgac gcgctggcag gcctgatcca cgacaagttc
    18361 ggcgaaggcg aataagcccg tcccgcactg cgcggaacaa cctgtctccc gtgacaaccc
    18421 ggggcgttca cggcatctcc cgctgctagg atggcccatg agtttccagt tgttcgggtt
    18481 gccggtgtcg cgcggtgtgg ccattggccg cgcggtgctg atctcgtcca gccgtgtcga
    18541 tgtggcgcat tacttcatcc gggatgccga tgtcgccccc gagatcgagc gcctgcggtc
    18601 ggcgcgggat gccgtggctt ccgagctcag cgcactgcag cgtgacatcc cgggcgatgc
    18661 gcctgctgag ctgtccgcgc tgctggacgt gcacctgatg ctgctcaacg acgagacgct
    18721 ggcaatagcg accaagcagt ggatccgtga gcgccactac aacgccgaat gggcgctttc
    18781 cgcgcagctc gaagtgttgg cgcgccagtt cgacgagatg gaagacgact acctgcgcga
    18841 gcggaaggcc gatgtcgagc aggtggttga gcgcatgctg cgcgcgatgg cccggggcag
    18901 cgcatccctg ctgacggatg cggccacgcc gacccaaggc gggacaccgc acagtgaacc
    18961 gctggtgatg gtggccaatg acatcgcgcc ggccgacatg ctgcatttca agcgcacggt
    19021 gtttgcggga ttcgtgaccg acgtgggcgg caagacttcg cacaccgcca tcgtggcgcg
    19081 cagtctggac atcccggcgg tggtcggggc ccgggagggc agtcgggtga tccggcagga
    19141 cgacctggtc gtggtcgacg gcgatgccgg tctggtcatc gtgaaccctt cggacatcgt
    19201 gctggaggaa taccggttca ggcagcgcga gtgcgaactc gcccagcagc ggctgtcccg
    19261 cctgcggcac acgcccgcgg tcaccctgga cggcgagcgc atcgagctga tggccaacat
    19321 cgaacttccc ggcgatgccg ctgcggctgt ggcggcgggt gcggtcgggg tggggctctt
    19381 ccgcagcgaa ttcctgttca tgaaccgtgc cggcgagctt cccggcgagg acgagcagtt
    19441 cgaggcctat cgcgcggccg tgctggcgat gaacgggttg ccggtgacca tacgcacggt
    19501 cgacgtcggt gccgacaagc cgctcgaccg gatgtctgtc aacgagttgc gtcacgagca
    19561 tgcgctgaac ccggcgctgg gcctgcgtgc gatccgctgg agcctgtcgg agccggccat
    19621 gttccgccag cagctccgtg cgatcctgcg tgcgagtgcc ttcggcaagg tgaagctgct
    19681 ggtgccgatg ctggcccatg tcggtgaggc aatgcagacg ctggacgcga tcgcgagggc
    19741 caagcagcaa ctcgtggatg ccggcaagcc gttcgtcgat gtcgaggtcg gagcaatgat
    19801 cgaggtaccc gcggcggcgc tggtgatgcc cagcctgctg aagctgttcg acttcgtttc
    19861 actcggtacc aacgacctga tccagtacac gctcgccatc gaccggggcg acgaatcggt
    19921 ggctcatctc tacgacccgt ggcaccctgc ggtgctcaag ctgatcgagg gcgtgatcca
    19981 ccatgcgcgc ctggctggca aggatgtcag cgtttgcggc gagatggcag gagacaccgc
    20041 attcaccgaa gtcctgctgg cgatgggtct gcgtaatttt tcgatgcacc cgtcgcagat
    20101 ctcgtcggtc aagcaggtgg tactgcgctg caacacccgc cgcctggcac ctctggtcga
    20161 gagcgcattt cgaggtgacg acctgtggac cggctgcgag cggttgcggg aatcggcgat
    20221 ggtggcctga ttccctagcg tcgcccgaac atcatctgcc gggtcagcgc ggacttcagc
    20281 ggtgacaggg tctgcagcgc cgccagtccc aaccctcgcg ccccgctcgc gcccggccat
    20341 tcccaggtga agctgcgggc cagaaaatcg gtggccgcga tcgtcgacca gcggtccggc
    20401 gcgcgcttgc ggtccaggcg gcgcagggcc tggtcgacat cgttcgatct caccagctct
    20461 cgcaccagca catgggcatc tcgcaggccg agattcagcc cctggccggc caccgggtgc
    20521 agggtctgcg cggcattgcc tatgcgcacg acgcggcctg gctcgcgatg cccgtcgcgc
    20581 tccacgccgc ctgtcagcgt gcgctccgca ttcagaccca gctgaaagcg cttgagcggc
    20641 gaaaccgagg tgatgcggcc ggcctccggc gggaagatcg tgttcaggac acagcagcgc
    20701 tgcacgtcgg tcagctcggc caccgggtcg tcgcccgcat cgacgcacca gaccagcgcg
    20761 gcctgatccg gtcccaaggg cagcagcgca gccggcccgt ggcgggtgaa gcgctcgtag
    20821 gcgacgccac cgcgtgcgcc gtccagtgtc acgcggccga cccaggcgac ctgcccgtag
    20881 tcgcgcgtga caggcttgcg ctcctgctcg gcgaagacgc cgccttcggc gatcaccgcc
    20941 agatcgaatg tctccgcgat gtcggcatcg acctcgacgc cgcccgggct cgacgcaccg
    21001 tctgccggct ccaccggctt cagcgacctg accggcgtcg agaaccgagt gatcaggcgt
    21061 tgcggcccgg ccttgcaagc ctccatccag acctgttgca aggccgctgt caccgtgccg
    21121 tacgacagca ccgcaccgag catcggcacg tccatctctg cagcccggat gcgcacctgc
    21181 ggctcgccca tccagcgccc cagcagcccg ccgaaggaag gcgcctgctg cgacacatgg
    21241 acttcggtga tgggttgccc catcccgtct tgccacgccc ccaggcgctg gagcagttgc
    21301 acgctgccga gcgacagtgc cagggtgcgc gggtcggccg atatatcggc gtcgatcgca
    21361 cgcgcatcga acacgctgat ctgggcgcgg ggcagtgcgc gagccgcctg tatggccagc
    21421 gacagtccga ccggtccggc gccgaccacg gccaggcgga tcgcacgatc gatcaaagga
    21481 gcggcttcat cttgcggatg cggggtcgtg ttcacggcgg gactttcgca ggcggtttcc
    21541 gtgcggggct tgatcgtagt gccggtcgtc ctggaggcgc ctggcgcgct tcagccgcgc
    21601 atcaaggcct cgatctcgag cggatcgact ggcgcgtcgc ggctgatcag ttcgcaaccc
    21661 ctggcactgt cggtgaccac cgcatcgtcc tcgatgcgga tgccgaggtt ccagtagcgc
    21721 tcgggtacgt ccgctgccgg gcgaacatag agtccgggct ccagcgtcac caccatgccc
    21781 gggcacagca cgcgtgacgg cctcttgacc agcgtgccgc cgtacccgtc gggctgttcg
    21841 aaagggtctt cttccagcga caggtactcg cccacatcgt gcacgtcgcg gccgagccag
    21901 tgaccggttc cgtgcatgta gaactggcgg taggcggcgc tggcgacgat gtcatccacg
    21961 tcgccgtgcc gatcgcgcga cagcagcccg aggtccagca tgccctgtgt cagcacgcgc
    22021 accgcggcgt gatgcgcatc acgttgccgc tgccccggcc gcgtgacctc gatcgcggct
    22081 tgctgggccg ccagcaccac cgcatacaac tcgcgctgtg gcgagctgaa gcggccgctg
    22141 gccggaaagg tgcgggtgat gtcgctggcg tagccgtcga gctcgcagcc cgcatccacc
    22201 aggcacaact cgtcgggtcg aagttcggcc gcgccggccg ggtgatgcag gatgcaggcg
    22261 ttggcgccag ccgcgacgat gctcgggtag gccgggctct gggcgccgag ccggcggaat
    22321 tcatgcagca gttcggcttc gagctcgtac tcgcgcaagc tggctgcgcc ttcccggaaa
    22381 cgctggccgc aaaagcgcat cacacgccgg tgggctccgg cagaaatcgt cgctgcgcgc
    22441 cgcatcgtgg tcagctcttc ggcgtccttg tggagtcgca tttcggcgat cagcgggtgc
    22501 aggtcgtgca gaacggtcgg gcattcgaca cccacgcgtt cccgcgtgcg caccttggtc
    22561 agccaggcct cgacctgcgt ctgcaactcg ggatggcgac cgaacggcaa cccgaccgct
    22621 cgccggttcg cgagcatcag cggcaactca gcgtccagcg attcgatggg ccgcgcctcg
    22681 tcgacgccga gtgcggcggg tgccgtctcg acgcccagcc gcgggccctc ccaggcctcg
    22741 cgggcagggt cgaccgggcg gcaaaagagc gtgctgtgac catcgctgtg cagcacgagc
    22801 caggcttccg gctcgtcgaa gccggtcagg tagtggaagt gactgtcatg ccgatagggg
    22861 tgatggctgt cggcattgcg ctggcgctcg ggagccgtgg gaacgacggc gatgtcgatg
    22921 ccgacgctgt gcagcctctg cgccagcatc gcgcggcgcg cggcatgggt cgagtgggtc
    22981 atcgggccac tatagccatg cgccgcttgc cagcgttcca tgcgatgcct cagctctgga
    23041 agcttctggc ggattacccg taccgcggtc gtttacgctt cacggatcga tcgttgccag
    23101 gatgcagatg aaagcccaca cccatgacat gagcccgtgg cagcacagcc atgtgttcga
    23161 cagcggaaac cggcggggcg aggcgcgcac gcgggtggtg cttgcggtca cgttgatcac
    23221 gatggtcgcc gagatcgttg gcggctggtg gacgggttcg atggcgttgc tggcagatgg
    23281 ctggcacatg ggtacccatg ctctggcgct cggtgtggcg gccgtggcct acgcgctggc
    23341 gcggcgccag gcgctcgatg ggcgctatgc cttcggcacc tggaagatcg aggtgctggg
    23401 tggctttgcc agcgcactgg ttctggctct tgtcgctgcg ggcatcaccc tggagtctgc
    23461 cctgagactg tggcgtgccg agcccatcga ggccgtgacc gcgctgtggg tggcggtcat
    23521 cgggctggtg gtgaacctcg tgtcggcatg gttgctgcac ggcgcggacg atgcgcatgg
    23581 ccatgatcac gcgcatgggc cggtcgatcc gcatgatcac ggacacgacc accatgcccg
    23641 tgctgaagat cacgcccacg cccatcccca ggcgcagtct gccgccgcac ctgtgggcgg
    23701 cgggcacgac ctgaacttgc gtgctgccta tgtccatgtg ctggccgacg cgctcacgtc
    23761 gttgctggcg atcgccgcgc tgtccggcgc tctctggctg ggctggtggt ggctggatcc
    23821 gctggtcggt gtgctgggcg ccgtggtcat cggggtgtgg gcggccgggc tgatgcgtca
    23881 gaccgcggca gtcctgctcg accgcgagat ggatcatccg ctgaccgcgc agatccgcgc
    23941 ggcgatcgag tcggacggcg atgcacgcgt ggccgacctg catctgtggc gtgtcggccg
    24001 ccagcactac gcggccatcg tcagcatcgt tgccgatgct ccgttcgctc ctgcggtcta
    24061 ccgcacccgg cttgcggaat atggcgcgct ggcgcacctg tcgctggagg tcaaccggtg
    24121 tcctcgtgcc gatgaatgtg acgccgaacc tgcggccgaa tccctgacca cgctgccgct
    24181 cgtgaagcgg cactgaactg ctcccgtcag gccgcgtcgg cgttcagcgc tgccagctga
    24241 gccggcgttc ccacgttctc ccacgctcct gggtagacct cggcggtgat gcggcgctcg
    24301 cgcatcgcgg cgtacagcag cggtccgagc gcggcacggg tgccgggggc cacctcgcga
    24361 aacagggcag cgcggcacaa ggcgatgttg gcgtaggtcc agcgcaaacc gtcagggccc
    24421 ggtccatcgg cgagcgcgtg gccgtcggcg gacaggccga aatcgccgtt cgggtggtaa
    24481 accggattcg gtaccagcca cagatgggca tcgtgatgtc ctgcagcgaa gcgtcgcccc
    24541 acctcggcat cgaaccggaa tccgggcacg tggatgtctc cggataccac ccagaagcag
    24601 tctgaatcca gctggcctgc catgccgcgc aacaacggca gtgcggtggc gatgccgccc
    24661 gcggtttcga gtgcaccgcc gtggtcgcgg ccctcgcacg agtagtggat ggtcaggccg
    24721 aagcgtgaac cgtctcccag cgtctcgacg attcgatcct cgagccaagc ggtgttgacc
    24781 accacgtcac gcacgccatc gcgcgccaga ccttcgaggt gccattcgat cagtggcttg
    24841 ccgtgcaccg ccagcagcgg tttcggcgtg gcgtcggtca gagggcgcat ccgttcgccg
    24901 cgaccagcgg ccaggacgat ggccctcacc gagggcgccc gcccaagcaa gttgcggtgc
    24961 caagaagcag catgaagcga tcgacgggga gggatgcgcc agacattgct cgcattatcg
    25021 gagcgcctgt ccggccatcg gtccggtccg accgaaagcc cggcgggctc ctgcctaaaa
    25081 tgccgggttc cgcaacacct tcgcatcagg gatacccatg gccgcagcag cgtcagacca
    25141 aaacacctcc ctgatggcaa atgccatccg tgcactggcc atggacgccg tgcagcaggc
    25201 caactcgggg catcccggtg cgccgatggg catggccgac atcgcggtgg cgctctgggg
    25261 ccggcacctg aagcacaacc cgggcaatcc gcactgggcc gatcgcgatc gcttcgtgct
    25321 gagcaatggt cacggttcga tgctgatcta cgccctgctg catctgacag gctatgacct
    25381 gccgatgagc gagctgcgca atttccgcca gttgcacagc aagacaccgg gccacccgga
    25441 agtcggcgtg acaccgggtg tcgagaccac caccggcccg ctcggtcagg gcatcaccaa
    25501 cgcggtaggc atggcgctcg ccgagaagct gctcgcagcc gagttcaatc ggccgggcca
    25561 caccatcgtc gatcaccaca cctacgcctt cctgggcgac ggttgcctga tggaaggcat
    25621 cagccacgaa gcctgcgcgc tggcgggggc ctggcgcctg aacaagttga tcgccttgta
    25681 cgacgacaac ggcatcagca tcgatggtca ggtcaagccc tggttcatcg acgacacgcc
    25741 gaagcggttc gaggcctacg gctggaacgt catcgccaat gtgaacggcc acgatgtcga
    25801 cgcggtcgac gccgcgatcg tgcaggccaa gagtgctggc aagccgacgc tgatctgctg
    25861 caagaccacc atcggcaagg gttcgcccaa tcgtgggggc accgccaagg cgcacggcga
    25921 ggcgctgggc gccgaggaga tcaagctgac gcgtgaggcg ctgggctgga cgtccgaacc
    25981 gttcgtgatt cccgagaccg tgtacgagca gtgggatgcg cgccacagtg ggctggccgc
    26041 cgaatcgaaa tgggtcgatg ccttcgaggc ctatggcaag gctcatcccg aactggcact
    26101 cgagtacgtc cgccgcgtcg agggccatct gccgaagaac ttcgctcagg tggcggtcga
    26161 cgccgccctg gccgcgcaca ccaaggccga aaccgtggcc agccgaaagg ccagccagat
    26221 cgcgctcgag gcgttcacga aagccatgcc tgaactgctc ggcggcagcg ccgacctgac
    26281 gggctcggtg ttcaccaaca ccagcagcac gcctgcgctg cgttttggcg atgacggttc
    26341 gagcaacggc gggcgccaca tcaactatgg cgtgcgcgag ttcggcatgg ccgcaatcat
    26401 gaatggcgtc gcgctgcatg gcggcttcat accgtacggc ggcaccttcc tgaccttcag
    26461 cgactacagc cgcaatgcgg tccgcatggc cgcactgatg aagctgcgcg tggtgcatgt
    26521 cttcacccat gacagcatcg gtctcggaga ggacggcccg actcaccagt cgatcgagca
    26581 tgttgcgtcg ctgcggctga ttcccgggct cgatgtctgg cgtcccgccg acaccgccga
    26641 gacggccatc gcctgggcct gcgcgcttga aagccgtcaa cggccgaccg cgctgatcct
    26701 ctcgcgtcac aacctgccgt atgcaccgaa gagcgggctc gacgacatct ccaagggcgc
    26761 ctacgtgctg gccgaaccga gcgaggtcgg gctgaagaag aaggcccagg ccgtcatcat
    26821 tgccaccggc tccgaggtca gtctggcgct gcatgcacag caactgctgg cgcagcagaa
    26881 ggttgccgtg cgcgtggtct cgatgccttc gacatcggtg ttcgaccgtc agagcgtggg
    26941 ctacaagacc agcgtcctgc ctgcgggctt gccgcgcatc gcggtcgaga tgggcgtcag
    27001 tgacggttgg tggaagtacg gctgcgccgc agtggtcggt atcgatacct acggcgagtc
    27061 ggcgcccgcg ccggtgctct tcaagcactt cggcttcacg gccgagaacg tcgtggccac
    27121 cgtgctgcgt gcaatttcgc gatgaaggct gcttggctct cccttgccgt ggtgctgacc
    27181 ggagtcagcg cagtggccga agtctgtgcg cagacgatcc tgcagatccg tgtcgtgcca
    27241 gccaagccga aagcaggcga gcccgcgacg ctggcaggac ggttcggccc gggagccgct
    27301 gctccgaact gcaatgtgcg ggtggacttc ggtgacgaga caccgaccca ggacgttctc
    27361 atcaaggatg cgaccgaact tccgctgatg ctcgctcatg tgtacgcgca gggtggcgac
    27421 tacgtcgtgt cggtgcaacc cgtgaccaag ggcaaggtgc ctgcgtgcag cggaggcaac
    27481 cacagcacct tgatcgaagt tcaggcgcct tgaatcgcgc gccgatttac tccccgaatt
    27541 caaaacccag aaaagacagg accctccaat gaccatcaag atcggtatca acggcttcgg
    27601 ccgcatcggg cgcatggtgt ttcgtgctgc cgtacagaac ttcgcggaaa tcgagatcgt
    27661 cggcatcaac gacctgctgg agcccgacta cctggcctac atgctccagt acgactcggt
    27721 gcacgggcgt ttcaagggca cggtctcggt cgaaggcagc acgctgatcg tcaatggcag
    27781 gaagatccgt ctgacggctg tgaaggaccc ggccgaactg aagtggaacg agatcggcgc
    27841 ggacatcgtg gtcgaggcca caggcctgtt cctgaccaag gaaacgggtg ccaagcacct
    27901 tgccgcgggc gcaaagaaag tgatcttctc ggccccttcg aaggacgaca cgccgatgtt
    27961 cgtctacggc gtcaatcatg gcgcctacaa gggagaagcg atcatctcga acgcttcgtg
    28021 caccaccaac tgcctggcac ccgtcgccaa ggtgttgaac gacaagtggg gcatcaagcg
    28081 cggcctgatg acgaccgtgc acgctgccac cgccacgcag aagaccgtcg acggcccgtc
    28141 caacaaggac tggcgcggtg gccgcggcat cctggagaac atcatcccgt caagcacggg
    28201 cgctgccaag gccgtcggcg tggtgattcc tgaactcaac aagaagctga ccggcatgtc
    28261 gttccgcgtg cccacctc
//
LOCUS       DAOORV010000035        27506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2053_length_27506_cov_8.311245, whole genome shotgun sequence.
ACCESSION   DAOORV010000035 DAOORV010000000
VERSION     DAOORV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_27506_cov_8.311245"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKV56_06790"
     CDS             <1..486
                     /locus_tag="PKV56_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOX67556.1"
                     /translation="AVLTTFVALLALIVSIPLWFVPPLVLILPPLIWGWLTYKVMTYD
                     VLAEHATKEERRELVKRHRSSLLGMGILTGYLGAAPSLVWASGVLFLALAPVLVPVAV
                     WIYTLVFAFAALWFSHYALSALQVLRAERDLATGRPEPGSPSSVIEVDAPAALLPLPP
                     A"
     gene            497..1324
                     /locus_tag="PKV56_06795"
     CDS             497..1324
                     /locus_tag="PKV56_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOX67557.1"
                     /translation="MAFGLLIVGDEILSGKRADKHVPKVIDLLAARGLSLSWVRYVGD
                     DPLRITSDLHDALASGDIVFSCGGIGATPDDHTRQCAARALGVPLALHPIARDLIFER
                     MRDVAAEKGEPFLPERADNVHRLNMAMFPEGAGIIPNPYNKIAGFFVRATARPDAHPD
                     AAVYFVPGFPVMAWPMIEWVLDQRCAHLHRAQGQREQSVIVLGAMEAALTPLMESVEA
                     RYPGVKVFSLPSVDHPEWGAHIELGVKGECMDVEEAFRTLMGGLRERHARLGPEMVR"
     gene            1603..3018
                     /gene="glnA"
                     /locus_tag="PKV56_06800"
     CDS             1603..3018
                     /gene="glnA"
                     /locus_tag="PKV56_06800"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOX67558.1"
                     /translation="MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDED
                     KFTSGHAFDGSSIAGWKGIEASDMQLMPDANTANIDPFFEEPTMILTCDVIDPADGKP
                     YERDPRSLAKRAEAYMKASGLGDTAYFGPEPEFFVFDSVRWGNDMSGCFSKIDSEEAS
                     WNTGREYEHGNTGHRPAVKGGYFPVPPVDSFQDMRSEMCLVLEALGVPVEVHHHEVAN
                     AGQMELGTRFSTLIQRADWVQLQKYVIQNVAHSYGKTATFMPKPIVGDNGSGMHVHQS
                     VWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSAKNRSASIRIPYVANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPG
                     EAATKDLYHLPPEEDKLIPTVCHSLDQALEYLDGDRAFLTKGGVFTDAYIDAYIDLKM
                     TEVTRFRMATHPVEFDMYYSL"
     gene            3172..3690
                     /locus_tag="PKV56_06805"
     CDS             3172..3690
                     /locus_tag="PKV56_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67559.1"
                     /translation="MMSLRGSRLFKGGFIAASLLLSVFTVQAQGDTAVVYRCPGNLYT
                     STITAKEAREQGCRTLDGAPITVLAAPKRPAAASGNAPTASPRPDTRIDPSEQRARDS
                     DARRILEAELQREEQRLSEMQAEYNKGEPERRGDERNYQKYLDRVSDLKSGIARKESD
                     IAALKRELSKLP"
     gene            3728..4798
                     /gene="glnL"
                     /locus_tag="PKV56_06810"
     CDS             3728..4798
                     /gene="glnL"
                     /locus_tag="PKV56_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HOX67560.1"
                     /translation="MTIAAAPYAAFDHLATMVAVVRPNGHCVFANASFGYVLGWSSRS
                     VLRDSLFDWFVDSQIVRDTVAAVVRNEFSTSRFEALLRRPASTHGEPLPVHVIVNQMD
                     GTTDVLIELVEIEQQTRQDREERALGQARANKELIRNLAHEIKNPLGGIRGAAQLLEM
                     EVESRALTEYTQVIIQEADRLQALVDRLLAPHRKPHVVCDVNIHEVCERVRSLILAEF
                     PRGLRVERDYDTSIPDFRGDREQLIQAVLNIAHNAAQALTEQMATGDAAITLRTRVAR
                     QVTLGKQRYRLALELHILDNGPGIPESIRDRIFYPLVSGREGGSGLGLTLAQTFVQQH
                     QGMIECESEPGRTIFKIVIPLP"
     gene            4852..6417
                     /gene="ntrC"
                     /locus_tag="PKV56_06815"
     CDS             4852..6417
                     /gene="ntrC"
                     /locus_tag="PKV56_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HOX67561.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALAREDLAVRSFTNPRDVLIALEED
                     VPQVLVSDIRMPGGSGIDLLAKVKQLHPELQVIIMTAYSDLDSAVSAFQGGAFEYLPK
                     PFDVPKAVELIRRALDESMRESVRDERLAEMPEMLGQAPAMQDVFRAIGRLSQSIVTV
                     LITGESGSGKELVAHALHKHSPRATGPFVAINTAAIPKDLLESELFGHERGAFTGAQT
                     TRRGRFEQADGGTLFLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHNPMRSNVRVIAAT
                     HQNLEDRVKQGVFREDLFHRLNVIRLRLPALRERQEDVPALARFFLQKSAKELGVEAK
                     RITDSAVALMMRFDFPGNVRQLENICHWLTVMAPAQTIEPKDLPPELLSLGVSAALPA
                     ALAGSPATATAFFSSSVPAASMPEPVSAGAVLPDAMAKPLAAEVPRDPLSSAEVPDGP
                     SRWLVDLERESRSLLLAGEPGVWDTLTRKFEAQLIHTALDMTRGRRIEAAQKLGIGRN
                     TITRKIQELGLDD"
     gene            complement(6436..7227)
                     /gene="xth"
                     /locus_tag="PKV56_06820"
     CDS             complement(6436..7227)
                     /gene="xth"
                     /locus_tag="PKV56_06820"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOX67562.1"
                     /translation="MRLATWNVNSLTVRLPQVLGWLAEHQPDALCLQETKLTDDKFPH
                     EELKAAGYESQWFGQKTYNGVALLTRGPVDAVVKNIPGYSDEQSRVIAGTVNGVRVIG
                     GYFPNGQAPDSDKFQYKMAWLEGLNAWVADELKTHSNLVLVGDFNIAPEDRDVHDPVA
                     WAGQIHCTPEERAHFQRLLSLGLHDAFRLFDQPARSWSWWDYRNLAFRKNQGLRIDHI
                     LVSDALKPGVTACSIDKLPRKNERPSDHAPVWVELDAAAGRSGEG"
     gene            complement(7526..8275)
                     /locus_tag="PKV56_06825"
     CDS             complement(7526..8275)
                     /locus_tag="PKV56_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67563.1"
                     /translation="MNNIPLHGLLVVVALAATPTAGAGPLFIGGTFSGIASVRQLPLN
                     FTPPHPMSYYEGATVTGSFEVFAPNPQPVSFSDSSFYNLGGWLSLSYTVSGETFTYLK
                     ESTRPPGSPSILILSPSGAGHALQSVFFATDWTNRYDGASIELSGMPGGLFEGIDVAT
                     LHFDPSQPLSMHTGMSSSAAEMRMDVDVRQFTVRSLNAVPEPAPAALLVLGLALLCAS
                     RWRVSRTGNPESKAPLQPLPAAAQSHADRGA"
     gene            complement(8433..11525)
                     /locus_tag="PKV56_06830"
     CDS             complement(8433..11525)
                     /locus_tag="PKV56_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX67564.1"
                     /translation="MKSFNLSEWAVTHRPLVLFLMLATLLVGGWSFSKLGRLEDPIFN
                     VPTMTAVVAWPGASARQVQDQVLNRMERELQEIEDIDYVRSFSRQGYGGLTLWMKGGT
                     SKAALDTAWYQARKKIGDVRAQFPEGVRGPFFNDEFTDVYTVIYALHAPELSPAETQV
                     LAEDIKRSLQAVAGVNKVDVMGRQVEQVNVEFESRRLASLGVKPHAMIESIGRQNMLE
                     PAGSVDGAADRTFVRTSGALRDAADVAAIPIEAGGRLLRLSDVAEVTSGYEDPPRFTV
                     RHNGEPVIALGVVLSRQVNLLKVGEQLDAATAALQAGLPLGVSLEKYADQPRVVDEAV
                     WEFEKAFLEALAIVLAVCFLALGWRSGIVVAASVPLVLGIVAAVMHAAGWALDRISLG
                     ALIIALGLLVDDAIIAVEMMVVKIEQGWDRARAATFAYSSTALPMLTGTLVTVAGFMP
                     VGFAKSIAGEYAGGIFWVVGVALLASWIVAVVFTPYLGVLLLPRSRSDKAAAAHDASP
                     YPRLRRTVEWALAHRWSVLGAAALLFLLAGAGMGLVQQQFFPSASRSEVLVELRLREG
                     ASFTATAAEVRRFERVLEADPDVKDYTAYTGAGSPRFFLSLGPELPNPGYAQFVVQTA
                     GIPEREALRKRLIALFDAGEAFPEVRGRVQRMEFGPPVGFPVQFRVIGPDAQQVREIA
                     HRVRDIVRGSPLVRDTHLDWDEQVRTVQVQVDQDQARLLGLSSAEVSNTVQAALSGVT
                     VTQIRRGEDLVDVVLRATPGERLSLDQLQDINLPTRNGNTVPLSQVARLDAAFEDPVL
                     WRRNRDLALTVRSDVADGIQGPYATQQIWPGLRPVLDTLPPGYRIEQGGAIEESDKSN
                     VALFAVFPAMLVVTLLILMVQLQSFSRMFMVFLTAPLGLIGVVPALLAFNAPFGFVAL
                     LGVIALGGMIMRNAVILVDQIDHDIAQGSAPWQAIVEATVRRARPVVLTAAAAILAMI
                     PLTRSIFWGPMAMAIMGGLVVGTALTLVFVPALYAAWFKVRPVNPAAAIETKAAPAEA
                     "
     gene            complement(11522..12712)
                     /locus_tag="PKV56_06835"
     CDS             complement(11522..12712)
                     /locus_tag="PKV56_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX67565.1"
                     /translation="MNRLPTPHAPRGGLVALAASSVAALLVGCSEAVAPTPVAQAVYV
                     TPVRLEHAAGERRYTATLAPRVESEIAFRTGGKVLSRLVDVGQRVRAGQPLARLDPAD
                     LELAVEAAAEQLRAAEVESNQAASDAARFKRLLADGSVGAADQERLQARADGAAARVI
                     QSRRQLDLARNRSSYTTLVAPFDGVLTSRRFEAGQVVGEGQAVLTLARESDLEIVADI
                     PERLAQELPSQVASARIGSGVPVAVRLRELSPGASAATRTFRARYAITQPRPETWRIG
                     MTAELLLAPAGSAESAAELPATALVSAQGQPAVWIVADPAAGRLALQPVRVVTQGSDR
                     VRVSGVADGALVVSAGAHKLDAAMRVRPVQRPLDAPLPAPTSDTKSVPAEVASAPKAA
                     ASKP"
     gene            complement(12709..14247)
                     /locus_tag="PKV56_06840"
     CDS             complement(12709..14247)
                     /locus_tag="PKV56_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOX67566.1"
                     /translation="MNRRSPPARPDPFPAAQCASARAGLHRTVRFAACLCIVVLAAAC
                     ASPVDGPDTRSARNAVQTAEFRHQGSDAGSFDPNWWAAFNDPVLSALVTEAQQANLDV
                     RIAATRVLQARAGSTAAASRLAPTLGLNGSVSDQRSGLPDAYKRTSPDTRATRVSLDL
                     GWEIDVFGAARASADAADFDAQASAEAAEGARLLASSEVARLYFAWQGARLRQQQFEE
                     LLQMRLDTERLIRNREAQGLASRFDVARAAAETQSLAAQLPPLRTLVATTGHQISLLL
                     GRSATQPLPMLDERVAPRLPEVPALPPGQPAELLARRPDLRAAERQLAAEGARVREAR
                     ADLLPRFFLAALFGAQDLTVNSQALSPVRYSNVALAFSMPLFNAGRLRAAEDRATARE
                     RGSAMQYERAVLGAVQEVEDSLVALAQERERAHALDALQSQSRTALRHAESLRTEGQI
                     DLLQLLDAQRGVISAELARTEHRTQLALDAIQLYKALGGGWRMPDTKDAAAAGGRPIS
                     ASPQ"
     gene            14455..15066
                     /locus_tag="PKV56_06845"
     CDS             14455..15066
                     /locus_tag="PKV56_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX67567.1"
                     /translation="MPRKIEEPIELKEACVRAAREVIAEHGIEQLSLREVARKLGVSH
                     QAPYRHYPSRDHLLAEVMRRCFESFARSLDERQRSGEGAICDLESLGRQYLAYAAAHP
                     LEYRLMFGTPWPEPAEHPELVHDAVHAFDVLRGVLRSLYGDGAEMRSQVDLQAMFIWS
                     TMHGLASIMQSNAMDCLTLAPGAKAQAPEYVMDMIRLALGPCR"
     gene            complement(15155..16687)
                     /locus_tag="PKV56_06850"
     CDS             complement(15155..16687)
                     /locus_tag="PKV56_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HOX67568.1"
                     /translation="MPLPFTLRPLLRACALAFTVSALSTAAVAHPQTAAVQISRVWQY
                     AHALGGVAGQKAEIVAYDDRTDTLWVAGVVGVDVLDRSTGQLVAHIDVTNLGAVNSVA
                     IHDGLAALAIENGIDRSLPGFVVFYETRTRAQSGQPVVVGSLPDMLTFTPNGKRVLVA
                     SEATPNAGSQVDPAGSVSIVEVKSRSVKTLPIDPAIVGYDTLRQFPALGNGTTRPNMT
                     PYGPEPEYIAVDKEGRYAYVGLQEANGIAVLNLKTDSFEKIYSLGLKDFSLTGNEIDP
                     HDQENGSGVPGRIELRPAAVKGLYQPDTIAAYSHKDRTYLVMANEGDSREDSADERRG
                     SAGSGAAELVPEGNDLSRLTLSNVDSAAGALVTFGGHSFSIRDAAGTIVFDSGSQLDR
                     EAITRGIYDDGRSDNKGVEPEGVALLHIEGRVLAFIGLERTLKSAIAVYDITNPRDAR
                     FIDMIVSDGVLNDVSPEGLKAFRVGKRYFVAAAHEVSDTTSLFEIDLSKGRDRDHHSD
                     DH"
     gene            complement(17123..17410)
                     /locus_tag="PKV56_06855"
     CDS             complement(17123..17410)
                     /locus_tag="PKV56_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67569.1"
                     /translation="MIHVNAGSSLRALLDPAQKSSASVLEAQVTGRPITLIGLVDAGR
                     TWFKSSTGLDAAQPPRDISFCNSARASNAIKYGREGRQVEIGAGAAGNAAA"
     gene            17492..18727
                     /locus_tag="PKV56_06860"
     CDS             17492..18727
                     /locus_tag="PKV56_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX67570.1"
                     /translation="MTDSPSKFPRRRTFALILLSVLVIAGLSARWWLGPQVAAETVQR
                     RDFVQTVVASGRVETPHRVDIGAQITGTVVRVPVAEGQSVKAGDVLITLESAELHAVV
                     RQAEVSVVQAEARVRQLRELQAPVAEQALRQTQASLDNARSTLRRNQDLFQKGFIGEA
                     SFEDSRKAYELADAQVRSAQKQLDAARPAGSDHALAVAAVAQAQASAEAARARAAYAV
                     ISAPVSGTLIGRNVEVGDVVQAGKVLMTLSPAGRTQLVAEIDEKNLRLLALGQKAVAS
                     ADAYPQQRFDAELAYINPGINAQTGAVQVKLDVPAPPSVLTQDMTVSVDIEVAKRPGA
                     LLVPLSAIHDADGPEGTEAWVLRIEGGRAVRKVVRLGLRSGGWAELLDGLSEGDRVLP
                     SSASVLTGSRVRAAASSVN"
     gene            18736..19986
                     /locus_tag="PKV56_06865"
     CDS             18736..19986
                     /locus_tag="PKV56_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67571.1"
                     /translation="MFTAWVPFEWIVALRFLREGRLQTLFIVAGVAVGVGVIVFMSAL
                     LAGLQANFINRVLSAQAHIQLLPPQEITRPLRGDRGGAVLPGGEAEGAIVQPPLQRLK
                     SIDQWRSVAAQIRAMPEVLVVSPVAGGSALAIRGNVSRSISLNGIEPELYFRIVDLGA
                     KIVSGSARLTGTDILIGTELASDLGLDVGDKLRVSTASGAAIALTITGVFDLGNKGAN
                     ERSVFVALHTAQSLLGLSGGVSSLEVTVRDVYAAEAIAQRITASTGVEADSWIKTSAQ
                     FFAAISAQTLANTAIRFFVGLSVAFGIASVLVVVVVQKSREIGILRAMGISQGQILRV
                     FLLQGGLLGLGGAMVGSAIGAGALILWQRYARNADGTPLFPLVFDPHLFAAALLLAML
                     TGLVAAFAPALRAARLDPVVAIRG"
     gene            19979..20704
                     /locus_tag="PKV56_06870"
     CDS             19979..20704
                     /locus_tag="PKV56_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67572.1"
                     /translation="MADTSLLAAPAGLAARSIVVSLHGVRKAYNAGTPIETEVLHGID
                     LTLLQGEFCAVMGPSGSGKSTLLNIIGLLDRPSAGTVAVCGEETTSLDDRALTRLRGH
                     SIGFVFQYHHLLTAFTALENVMMPMLGAAGFPNDAMRQRAIELLDAVGLKDRLHDGAG
                     NLSGGQQQRVAIARALAMNPALLLADEPTGNLDTRTAGEAFSLLRRFNQEHGTTVLFV
                     THNPALAERCDKTIQVVDGLVDG"
     gene            complement(20713..21378)
                     /locus_tag="PKV56_06875"
     CDS             complement(20713..21378)
                     /locus_tag="PKV56_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67573.1"
                     /translation="MMKLLTCSIVAILAVSALAVGPTVAADRHDGRKPPPSRNTRAPH
                     LWLDQRFRHDHYYPRHGEVIPALPGGSISIGFGGGHFYFHGGVWFRALGGRFVVIAPP
                     IGVIVPLLPPAYATLWIGGAPYYYANGVYYALAPGQGYAVVDPPPGIENAQPAPLPAA
                     AAKPAPDPVIYPRNGQSTQQTDSDRQDCNRWATTQQNAMNDADVFNRAVAACMDARGY
                     TLR"
     gene            21584..24340
                     /gene="mgtA"
                     /locus_tag="PKV56_06880"
     CDS             21584..24340
                     /gene="mgtA"
                     /locus_tag="PKV56_06880"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOX67574.1"
                     /translation="MGTDWRKWFSELARRRRTLRHFGRHPLLATLAGRGPVASVPDHA
                     ASELLLVARAEPAAAIARLRSHADGLSPREAAARLRRHGPNEVAHEKPLPPWLHLWHC
                     YKNPFNLLLTALAAVSWLTQDAKATVVIGVMVILSTGIRFVQEGRSHRAADRLKAMVS
                     NTATVMRRPPDGADPAAPVAVPAPREIPQRELVPGDVVALSAGDMIPADCRVLTARDL
                     FVAQSAMTGESLPVEKFVQHEGEGGGLLEQGNLVFMGTNVVSGAATALVVATGNHTCF
                     GALAQSASANEAEPNAFHAGVNGVSWLLIRFAMVMVPIVLVVSGFAQGDWLQAFLFAL
                     SVAVGLTPEMLPMIVTSTLAKGAVQLSRKKVVVKRLDAIQNFGAMDLLCTDKTGTLTQ
                     DRIALARKSDPFGLPSDEVLKYAFLNSYYQTGLKNLLDHAVLEHVELATELKLKQDYR
                     KIDEVPFDFERRRMSVVVSLKSAELGEQHQLICKGAVEEVLAACTRLRIVDATGTRDV
                     ALDADMLSRVQRAAHQLNDEGLRVVAVAIKTLPAEQTTYAISDESELTLVGSIAFLDP
                     PKDSAAAALKALGAHGIRVVVLTGDNDLVAARVCHQVGLDASTVLLGSQVEAMDSAQL
                     AKAVEQHTVFAKLSPMHKERIVRALRANGHVVGFMGDGINDAPALHAADIGISVDTAV
                     DIAKEAADIILLEKSLLVLDEGVVEGRTTFCNMLKYIRMTASSNFGNVFSVLVASAFL
                     PFLPMLPLQLLVQNLLYDVAQTGIPFDNVDTELVGQPLKWDAGGIGRFMLFFGPISSV
                     FDLTTFAVMWWVFGADTVAEQALFHTGWFVVGLLTQTLVVHMIRTPKLPFIQSLASAP
                     LLAMTAVVMAVGVWLPMGPLAAHFGLQPLPLGYFPWLVGILSGYCVLTTLMKRLYIRR
                     YGWQ"
     gene            24420..27245
                     /locus_tag="PKV56_06885"
     CDS             24420..27245
                     /locus_tag="PKV56_06885"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOX67575.1"
                     /translation="MTYMEFAGDPSTMEATQVASALQVDVDQGLTQAEASKRLAQCGP
                     NALRATATLPAWRRWLSHFNDPLVFLLLGAAGVAFAAWVVEGRHGAPVDAIVIVLVVV
                     LNAVLGYLQEAKAASAVAALARMTATTCTVLRDGHQSRIPSAGLVPGDVMLLTEGDAV
                     GADARLIQVASAQLMEASLTGESQSVLKDAATLPAPVPLGDRLNMVFKGTAVAQGSAR
                     AVVTATGMHTEVGSVAALMDATRDAPTPLQQEVARIGRRLGMAALVVAAVVIVTILLV
                     SDIRSAADVIEVLLLGVSLAVAAVPEGLLAILSVVLAMGVQRMARENAIVKKLASVET
                     LGSATVIATDKTGTLTRAEMTIVRVMTASGGSHVTGVGYAPEGRVEHQGAVLTEGPVH
                     DEARLVLSGGSLAGNADLRQADDGAWAIQGDPTEAAFLVAERKLGTDAHRKRRFTRMA
                     EIAFTSDRKMMSTIETDHDQGDAPVLITKGAPDVLLARCTRARVGKAIVPLDDALRQR
                     VLADVDTLSDAALRTLAVAYRPLEPDESPQAEGLVAPSIERDLIYVGTVGIIDPPRTE
                     VALAIGEAHRAGIRVVMITGDHPRTALRIAHDLGIVGGDASALTGTDLDALDDAAWGE
                     AVRTTSVFARVAPKHKLRIVAELQAAGNVVAMTGDGVNDAPALKAADIGIAMGRTGTE
                     VTKEAANMILADDNFETIVVAVKEGRGILDNIKKFLRYLLSSNLGEVLTVFLGVVGAG
                     VLGLAGATGGVVLPLLATQILWINLITDAGPALAMGADPIAGDVMARPPRKRTERVID
                     ARMWAGVLQVGLVMAVVSLLTIDAYLPGGLIEGAHDLTLARTAGFTVLVFAQLFNCLN
                     ARSERASAFEGIFANRWLWGSILLSTLLQVAVVHVGVLNVAFGTTPLSIEQWLGCAAM
                     ASVVLWFSEVRKWVLRAVSRRGEPARR"
     gene            complement(27285..>27506)
                     /locus_tag="PKV56_06890"
     CDS             complement(27285..>27506)
                     /locus_tag="PKV56_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67576.1"
                     /translation="SAEVRCLARRGALQVGSPALTDNALRRCDPLPEAQRLACLARMQ
                     GQGSTSGSAATGGIFREISTPQNLSTLPR"
BASE COUNT         4481 a         9025 c         9239 g         4761 t
ORIGIN      
        1 gccgtgctga ccacgttcgt ggcgctgctg gcactgatcg tgtcgatccc gctgtggttc
       61 gttccgcctc tcgtgctgat cctgccgcca ctgatctggg gctggctgac ctacaaggtc
      121 atgacctacg acgtgctggc cgagcacgcg accaaggagg agcgccgtga gctcgtcaag
      181 cggcatcgaa gctcgttgct gggcatgggc atcctgaccg gatacctcgg agctgcgccg
      241 tcgctggtgt gggcctctgg cgtgctgttc ctcgcgctgg ctccggtgct ggttccggtg
      301 gccgtgtgga tctacacgct cgtgttcgcg ttcgcggccc tgtggttctc gcattacgcg
      361 ctcagtgcct tgcaggtcct gcgcgccgag cgcgacctcg cgacggggcg ccccgagccg
      421 gggagtcctt cgtccgtgat cgaggtggac gcgccggcgg cgctgctgcc gctcccacca
      481 gcctgagcac atcgacatgg cattcgggtt gctgatcgtc ggagacgaga tcctttcggg
      541 aaagcgtgcg gacaagcacg tgcccaaggt gatcgatctg cttgcggcaa ggggtttgtc
      601 gctgagctgg gtgcgctacg tgggcgatga cccgctgcgc atcacgtcgg acctgcatga
      661 cgcgctggcg agcggggaca tcgtgttctc ctgcggcggc atcggcgcga cgcccgacga
      721 ccacacgcgg cagtgcgctg ccagggccct cggtgttcct ctggcgttgc accccatcgc
      781 ccgagatctg atcttcgagc gcatgcgaga cgtggctgca gagaaaggcg agcccttcct
      841 gcctgagcgc gccgacaacg ttcatcgcct gaacatggcg atgtttccgg agggggccgg
      901 gatcatcccg aatccctaca acaagatcgc cggcttcttc gtgcgtgcca ccgcgcgtcc
      961 ggacgctcat cccgacgccg cggtctactt cgtgccgggc tttcctgtga tggcctggcc
     1021 gatgatcgaa tgggtgctcg atcagcgctg cgcccacctg caccgcgcac agggacagcg
     1081 cgagcagtcg gtcatcgtgc tcggcgcgat ggaagccgcg ctgacgccct tgatggaatc
     1141 ggtcgaagcc cggtatcccg gtgtgaaagt gttcagcctg ccgagcgtcg accatccgga
     1201 gtggggcgcg cacatcgaac tgggcgtcaa gggcgaatgc atggatgtcg aggaggcctt
     1261 tcgcacgttg atgggcggat tgcgtgaacg ccatgcccgt ctgggacccg aaatggtgcg
     1321 ttgactggtt ttggtgcgcc ccattgttgg gcgtcttatt gcacgcattc gggccttggc
     1381 tgaggttaaa ttccggcatt gctgtgtcgc gatgcgcgcg cggaaatgcc ccgttttcct
     1441 gcaggcaggc acggggtcga tgcgcaggtc gggtgcccca tgggcacgct ttctgctact
     1501 tctcagtgcg catttttgag gtggcttgtc agcgccgcag cagtgttcgt tccttcccct
     1561 taccccaaca cacccaaaca gcgtcccgct aggagattcc tgatggccaa gaccgtcgcc
     1621 gatgtgatga agatggtgaa agaaaacgaa gtcaagtttg tcgacttccg tttcaccgac
     1681 acccgcggca aagaacagca cgtcaccgtg cccgtgtccc atttcgacga agacaagttc
     1741 acgtcgggtc atgccttcga cggttcgtcg attgctggtt ggaagggcat cgaagcgtct
     1801 gacatgcagc tgatgcccga tgcgaacacg gccaacatcg atccgttctt cgaagagccg
     1861 acgatgatcc tgacctgcga cgtcatcgat cccgctgacg gcaagcccta cgagcgcgat
     1921 ccgcgttcgc tggccaagcg cgccgaggcc tacatgaagg cctcgggcct gggcgacacc
     1981 gcctacttcg gtcccgagcc cgaattcttc gtgttcgaca gcgtccgctg gggcaacgac
     2041 atgtcgggct gcttctccaa gatcgattcg gaagaggcat cctggaacac cggccgtgaa
     2101 tacgagcacg gcaacaccgg tcaccgtccc gcggtcaagg gtggttattt cccggttccg
     2161 ccggtcgaca gcttccagga catgcgctcc gagatgtgcc ttgtgcttga agccctgggc
     2221 gtgccggtgg aagtgcacca tcatgaggtg gccaacgccg gccagatgga actcggcacc
     2281 agattcagca cgctgatcca gcgcgccgac tgggttcagc tgcagaagta cgtcatccag
     2341 aacgtcgctc attcgtacgg caagacggcc accttcatgc cgaagccgat cgtcggtgac
     2401 aacggctcgg gcatgcacgt gcaccagtcg gtctggaaag atggcaagaa cctgtttgcc
     2461 ggtgacggct acgccggcct gtccgacttc gcgctctact acatcggcgg catcatcaag
     2521 cacgcccgtg cgctgaacgc gatcaccaac ccgggtacca acagctacaa gcgtctggtt
     2581 cctgggttcg aagcaccggt caagctggcc tactcggcca agaaccggtc ggcttccatc
     2641 cgcattccgt acgtggccaa cccgaagggc cgccgcgtcg aggcgcgttt cccggatccg
     2701 ctgatgaatc cgtacctcgg gttcgccgcc ttgctgatgg ccggcctgga tggcgtcgag
     2761 aacaagatcc atccgggcga agccgcgacg aaggatctgt atcacctgcc gccggaagag
     2821 gacaagctga ttccgaccgt ctgccacagc ctcgatcagg cgctcgagta tctcgacggc
     2881 gaccgcgcat tcctgaccaa gggcggcgtg ttcaccgatg cgtacatcga tgcctacatc
     2941 gacctgaaga tgacggaagt cacccgcttc cgcatggcga ctcacccggt cgagttcgac
     3001 atgtactaca gcctctgagg ctggcgtagc agaatcgagg gacggcgtga gccgtccctt
     3061 tttctttgga tggggaattt catcggtgat cgagaatccg agatgaatca agttggtgcg
     3121 cgtcgatgcg cagctcctgt ggaatcgagt gtttcgagag agggttatcg gatgatgtct
     3181 ttacgcggca gtcggttgtt caagggcggg ttcatcgcag ccagcctgct cttgtcggtg
     3241 tttaccgtgc aggcgcaggg cgacacggcc gtcgtgtatc ggtgccccgg caacctttac
     3301 accagcacga tcaccgccaa ggaggccagg gagcagggct gccgcacgct ggatggcgct
     3361 cccatcacgg tgctggccgc gccgaagcgg cccgcggcgg caagcggcaa cgctccaacc
     3421 gcatctccgc gtccggacac ccgcatcgac ccgagcgagc agcgagcccg cgacagcgac
     3481 gcgcgcagga tcctcgaggc tgaactgcag cgcgaggaac agcggctttc cgagatgcag
     3541 gccgaataca acaagggcga gccggagcgt cgcggcgacg aacgcaacta ccagaagtac
     3601 ctcgatcgcg tctccgacct gaaatccggc atcgcccgca aggaaagcga cattgccgcg
     3661 ctgaagcgcg aactgtcgaa gctgccttga tgcccgattt ccaggagcca cggtgcggtt
     3721 gagcccgatg acgatcgccg ccgcacccta cgcagcgttc gaccacctgg ccacgatggt
     3781 ggcggtggtg agaccgaacg gacactgcgt gttcgcgaat gcctctttcg gatacgtgct
     3841 gggctggtcg agccgcagcg tgctgcgcga ctcgctgttc gactggtttg tcgattccca
     3901 gatcgtgcgt gacaccgtcg ccgccgttgt tcgcaacgag ttctcgacca gccgcttcga
     3961 ggcgctgttg cgtcgtcctg cttcgaccca tggcgaaccg ctgccggtgc atgtcatcgt
     4021 caaccagatg gatggcacca cggacgtgtt gatcgaactg gtcgagatcg agcagcagac
     4081 ccgacaggat cgcgaagaac gcgcgctcgg tcaggcccgc gccaacaagg aactgatccg
     4141 caacctggcc cacgagatca agaaccccct gggcggcatc cgcggcgcgg cgcagctgct
     4201 cgagatggaa gtcgagtctc gtgcactcac cgagtacacg caggtcatca tccaggaggc
     4261 cgatcgactg caggcactcg tcgatcgcct gctggctccg catcgcaagc cacatgtggt
     4321 gtgcgatgtg aacatccatg aagtctgcga gcgcgtgcga tcgctgatcc tggcggagtt
     4381 tccgcgcggt ctgcgggtcg agcgcgacta cgacacctcg attcccgact tccgtggaga
     4441 tcgcgagcag ctcatccagg cggtgctcaa catcgcgcac aacgcggccc aggcattgac
     4501 ggaacagatg gccactggcg atgcagcgat caccttgcgc acgcgcgtgg cccgacaggt
     4561 gaccctgggc aagcagcgtt atcgactggc actggaattg catattctgg acaacggccc
     4621 gggcatcccg gaatccatcc gagatcggat cttctatccc ctggtgtcgg ggcgcgaagg
     4681 cggttcggga ttgggattga cgctggcgca gacgtttgtg caacagcacc agggaatgat
     4741 cgagtgcgaa agcgaacccg gcaggaccat tttcaagatc gtgattccgc tgccctgagc
     4801 tctttcctgg gcaccgcagt gtgtcggtgt cttccattga agaggcagtg aatgaaaccc
     4861 atctggatcg ttgacgacga ccaatccatc cgcttcgtgc tggaaaaggc cttggcgcgc
     4921 gaagaccttg cggtgcgcag cttcaccaac ccgcgcgatg tgctgatcgc gctcgaggag
     4981 gacgtgccgc aggttctggt ctccgacatc cggatgcccg gcggctcggg catcgatctg
     5041 ctggccaagg tcaagcaact ccacccggaa ctgcaggtca tcatcatgac cgcgtactcc
     5101 gacctcgaca gcgcggtcag tgcgttccaa ggcggcgcat tcgagtacct gcccaagccc
     5161 ttcgacgttc ccaaggcggt cgagctgatc cgccgagcgc tcgacgagag catgcgcgaa
     5221 tcggtgcgcg acgagcgtct ggccgagatg ccggagatgc tgggccaggc gccggcgatg
     5281 caggacgtgt tccgcgcgat aggtcggctg agccagagca tcgtcaccgt gctgatcacc
     5341 ggcgagtcgg gatccggcaa ggaactggtc gcgcatgccc tgcacaagca cagcccgcgc
     5401 gcgaccggtc cgttcgttgc catcaacacc gccgcgatcc cgaaggatct gctggagagc
     5461 gaattgttcg gccacgagcg tggcgccttc accggcgcgc agaccactcg acgtggtcgc
     5521 ttcgaacagg ccgatggcgg cacgctgttc ctcgacgaga tcggtgacat gccgttcgac
     5581 ctgcagacac gtctgctgcg cgtgctgagc gatggtcagt tctatcgtgt cggtggccac
     5641 aacccgatgc gcagcaacgt gcgggtgatc gccgcgactc accagaacct cgaagaccgg
     5701 gtcaagcagg gggtcttccg tgaagacctg ttccaccgcc tgaacgtcat ccgtctgcgc
     5761 ctgccagcgt tgcgcgagcg gcaggaagac gtgcccgctc tggcgcgctt cttcctgcag
     5821 aagagcgcca aggaactggg tgtcgaggcc aagcgcatca cggactccgc cgtggcgctg
     5881 atgatgcgtt ttgatttccc gggcaacgtt cggcaactcg agaacatctg tcactggctc
     5941 acggtgatgg cccctgcgca gaccatcgag cccaaggacc tgccgcccga actgctgtcg
     6001 ctcggcgtgt ctgcggccct gccggcggcg ctcgccggca gccccgcgac agcgacggcc
     6061 ttcttcagca gctcagtgcc cgcggcttcg atgcctgagc cggtgtctgc cggggcggtc
     6121 ttgcccgatg cgatggccaa gccgttggcg gccgaagtgc cgcgagatcc cctgtcgagt
     6181 gcagaggtgc ccgacggccc ctctcgctgg ctggtcgatc tcgagcggga gtcgcgcagc
     6241 ttgctgctgg cgggcgagcc aggcgtgtgg gacacgctga cccgaaaatt cgaagcgcag
     6301 ctgatccaca ccgccctcga catgacgcgg ggtcgccgca tcgaggccgc tcagaaactc
     6361 ggaatcggcc gcaacacgat cacgcgcaag atccaggaac tcggcctcga cgattgagct
     6421 ggaggcttca gccgatcagc cttcgccgga tcggcccgct gctgcatcca gttcaaccca
     6481 caccggcgca tggtcactgg gccgttcgtt cttgcgcggc agcttgtcga tcgagcaggc
     6541 cgtcaccccg ggtttcaagg catcgctgac caggatgtga tcgatgcgca ggccctggtt
     6601 cttgcggaac gccaggttgc gatagtccca ccagctccag ctcctggcgg gctggtcgaa
     6661 caaccggaac gcgtcgtgca gcccgagtga cagcagccgc tggaagtggg cgcgctcctc
     6721 gggcgtgcag tggatctggc cggcccaggc caccggatca tgcacatccc gatcctcggg
     6781 ggcgatgttg aagtcgccca ccagaaccag attcgagtgc gtcttcagtt catctgctac
     6841 ccaggcattc agcccttcga gccaggccat cttgtactgg aacttgtcgc tgtcgggcgc
     6901 ttgcccattc gggaaatagc cgccgatcac ccgcacgccg ttcaccgtcc ccgcaatgac
     6961 acgcgactgc tcgtcgctgt agccggggat gttcttgacg accgcatcga ccggcccgcg
     7021 ggtcagcagc gcgacgccgt tgtacgtttt ctggccgaac cactgcgact catagccggc
     7081 ggccttcagt tcctcatgcg ggaacttgtc atcggtcagc ttcgtttcct gcaggcacaa
     7141 cgcgtcgggc tgatgctcgg ccagccagcc cagcacctgg ggaaggcgaa cggtgagcga
     7201 attgacgttc caggtggcga gtctcatcgt tccgaaattt ctctgtgagt caataaactg
     7261 ccgaccagga ctttggtctc gccacgcgtc tggccacacg aatcgtcgtt gcgtcccgaa
     7321 ttgtgccgcg catggcgcgc tcggctctcg ggtgtccagc cgttcaccat gtcggcagcc
     7381 accagttggg atggcctagt tgggaaatcg cccgcggagg agccaatcgc atcgaatgtc
     7441 ggcctgctgg cagcgggacc gcagcttctt gccggtcacc cgctgtcagt cgcgggcagc
     7501 ggctcccgct agttggtgaa gatcctcatg cccctctgtc cgcatgcgat tgcgccgccg
     7561 cgggcagagg ctggagtggt gcctttgact ctggatttcc tgttcgcgac acgcgccacc
     7621 gtgacgcaca caacagggcc aggcccagga cgagcagcgc ggccggcgcg ggctccggca
     7681 ccgcgttcaa cgagcgcacg gtgaactgcc tgacgtccac gtccatccgc atctcggcgg
     7741 cgctactgga catgcccgtg tgcatcgaca gcggctgcga cgggtcgaag tgcagcgtcg
     7801 ccacgtcgat cccttcgaac aggccgcccg gcatgccgct cagttcgatc gaggcgccgt
     7861 cgtacctgtt cgtccagtcg gtcgcgaaga agaccgactg cagagcatgg cctgccccgg
     7921 agggcgacag gatcaggatg ctcgggctgc cgggtggccg cgtagattcc ttgaggtagg
     7981 tgaacgtctc tccactgacc gtgtatgaca gcgagagcca gccgcccagg ttgtagaagc
     8041 tgctgtcgga gaacgagacg ggctgcgggt tcggcgcaaa gacctcgaag ctgccggtca
     8101 ccgtcgcgcc ttcgtagtac gacatcggat gcggcggcgt gaagttcaac ggcagttgcc
     8161 gcaccgacgc gatgccgctg aacgtgcctc cgatgaacag cgggcccgcg ccagcggtcg
     8221 gggttgccgc caatgcgacg acgacgagca gcccgtgcaa tggtatgttg ttcatgcctg
     8281 aacggtgctg cattcgaacg ggctgcaccg ttcgccatcg cacacaccga gggatcgccc
     8341 tggcgaccgc tggcagcggc cgcctcactt gggccggatc gccccggcgt tctcgagctt
     8401 cggctgcgcc ttgcgctgga gactccagcc ggtcaagctt ccgccggtgc cgcctttgtt
     8461 tcgattgcag cggcaggatt caccggacgg accttgaacc acgcggcata gagcgccggc
     8521 acgaagacca gggtcagcgc cgtgcccacc acgaggccgc ccatgatggc catcgccatc
     8581 ggtccccaga agatgctccg agtcagcggg atcatcgcga ggatcgcggc cgcagccgtc
     8641 agcacgaccg gccgcgcacg tcgcaccgtg gcctcgacga tcgcctgcca gggcgcgctg
     8701 ccttgcgcga tgtcgtggtc gatctgatcg acgaggatga ctgcgttgcg catgatcatc
     8761 ccgccgagcg cgatcactcc gagcagcgcg acgaagccaa atggcgcatt gaacgcgagc
     8821 agcgccggca ccacaccgat cagacccagt ggcgcggtca ggaacaccat gaacatgcgc
     8881 gagaagctct gcagctgcac catcaggatc agcagcgtca cgacgagcat cgcagggaac
     8941 accgcgaaca gcgccacgtt gctcttgtcg ctctcctcga ttgcaccccc ctgttcgatg
     9001 cggtagcccg gcggcagcgt atccagaacc ggcctgaggc ccggccagat ctgctgcgtg
     9061 gcgtaggggc cctggatgcc gtcggccacg tcgctgcgca cggtcagcgc gaggtcgcgg
     9121 ttgcgtcgcc acagcaccgg gtcctcgaag gccgcgtcca ggcgcgccac ctgcgacagt
     9181 ggcaccgtgt tgccgttgcg ggtgggcagg ttgatgtcct gcaactgatc gagggacagg
     9241 cgctcccccg gggtggcgcg cagcacgacg tcgaccaggt cttcgccgcg cctgatctgc
     9301 gtcaccgtca cgcccgacag cgccgcctgg acagtgttcg acacctcggc cgaggacagt
     9361 cccagcaatc gcgcctggtc ttgatccacc tgcacctgca ccgtgcgcac ctgttcgtcc
     9421 cagtcgagat gggtgtcgcg aaccagcggg ctgccgcgca cgatgtcgcg cacacggtgg
     9481 gcgatctcgc gcacctgctg ggcgtcgggt ccgatcacgc ggaactgcac cggaaacccg
     9541 accggcgggc cgaactccat ccgctgcacc cggcctcgca cctcggggaa ggcctctcct
     9601 gcgtcgaaga gcgcgatcaa tcgcttgcgc agcgcttcgc gctcggggat acctgcagtc
     9661 tgcacgacga actgcgcata gcccgggttc ggcagttcgg gccccaggct caggaagaag
     9721 cgcggcgagc cggcgccggt gtaggcggtg tagtccttca cgtcggggtc ggcctcgagc
     9781 acgcgttcga agcgtctcac ttcggctgcg gtggcggtga acgacgcgcc ttcgcgcagc
     9841 cgcagctcga ccagcacttc gctgcgcgac gcgctcggga agaactgctg ctgcaccagc
     9901 cccatgccgg caccggccaa caggaacaac agcgctgcag caccgagcac ggaccagcgg
     9961 tgcgcgagtg cccactcgac ggtccggcgc aggcgggggt agggcgaggc atcgtgtgct
    10021 gctgccgcct tgtcgctcct tgatcgaggc agcagcagca caccgaggta gggcgtgaag
    10081 accaccgcga cgatccacga ggcgagcagc gcgactccca cgacccagaa gatgccgccg
    10141 gcgtattccc cggcgatcga tttcgcgaac ccgaccggca tgaagccggc caccgtcacc
    10201 agcgtgccgg tgagcatcgg cagggcggtc gagctgtagg cgaaggtcgc ggcgcgggcg
    10261 cggtcccagc cctgttcgat cttcaccacc atcatctcca cggcgatgat cgcgtcgtcg
    10321 accagcaggc cgagcgcgat gatcagcgcc ccgagcgaga tgcggtcgag cgcccaaccc
    10381 gccgcgtgca tcaccgcagc gacgatgccc agcaccagcg gcaccgacgc cgcgaccacg
    10441 atgccgctgc gccagcccag cgccaggaag cacaccgcga gcacgatggc cagcgcctcc
    10501 aggaacgcct tctcgaactc ccacaccgct tcatcgacga cgcgcggctg atcggcgtat
    10561 ttctccaggg acacgcccag cggcaagccg gcctgcaggg ccgcggtggc ggcatcgagc
    10621 tgctcgccaa ccttgagaag attcacctga cgcgacagca cgacgccgag cgcgatcacc
    10681 ggctcgccgt tgtggcgcac ggtgaacctc ggcgggtctt cgtagcctga tgtcacttcg
    10741 gccacatcgg acagccgcag cagccgaccg cccgcctcga tggggatcgc ggcgacatcg
    10801 gccgcatcgc gcagtgctcc gctggtgcga acgaaggtgc ggtccgctgc gccatcgacc
    10861 gagcccgctg gttcgagcat gttctgtcgt ccgatcgatt cgatcatcgc gtgcggcttc
    10921 acccccagcg aggccaatcg cctggactcg aattcgacat tcacctgctc gacctggcgg
    10981 cccatcacgt cgaccttgtt cactcccgcc accgcctgca gcgagcgctt gatgtcctcc
    11041 gccagcacct gcgtttcggc cgggctcagc tcgggcgcgt gcagcgcgta gatcacggtg
    11101 tagacgtcgg tgaactcgtc attgaagaac ggtccacgca cgccttcggg gaactgtgct
    11161 cgcacgtcgc cgatcttctt gcgcgcctgg taccacgcgg tgtccagcgc ggccttcgac
    11221 gtgccgccct tcatccacag cgtgagcccg ccatagccct gccgggagaa gctgcgcacg
    11281 tagtcgatgt cctcgatctc ctgcagctcc cgctccatgc ggttgagcac ctgatcctgc
    11341 acctgtcgtg cgctggcgcc gggccaggcg acgacggccg tcatcgtcgg cacgttgaaa
    11401 atcgggtctt ccaggcgccc gagcttcgag aacgaccagc cgcccaccag cagcgtggcc
    11461 agcatcagga acagcaccag cggccggtgc gtgacggccc actcggacag gttgaatgac
    11521 ttcatggctt tgacgcggcc gccttgggtg cgctcgcgac ctcggccggc accgacttcg
    11581 tatcggatgt cggcgctggc agtggtgcat cgagaggccg ctgcaccgga cgcacgcgca
    11641 tggcggcatc cagcttgtgc gcacctgccg agaccaccag cgctccgtcg gcgacaccgc
    11701 tcacgcgcac gcggtcgctt ccctgcgtca ccacgcgcac cggctgcagc gcaaggcggc
    11761 ctgcggccgg gtcggccacg atccacaccg cgggttgacc ttgcgccgag accagcgccg
    11821 tcgcgggcag ctcggcggcc gattccgctg aaccggcggg tgccagcagc agttccgccg
    11881 tcatgccgat gcgccatgtc tcggggcggg gctgcgtgat cgcgtaacgg gcgcggaacg
    11941 tacgcgtcgc cgcgctggcc cccggtgaca gctcgcgcag ccgcacagcg acaggcacac
    12001 cgctgccgat gcgcgcgctc gcgacctgcg agggcagctc ctgtgcaagc cgttcgggaa
    12061 tgtcggcaac gatctccaga tcgctctcgc gcgccagcgt gagcaccgcc tgtccctcgc
    12121 cgaccacctg cccggcctcg aagcgccgcg aggtcagcac cccgtcgaac ggcgcgacca
    12181 gcgtcgtgta gctgctgcgg ttgcgtgcca ggtcaagctg tcgccgggac tggatgacgc
    12241 gtgcggcggc tccgtcggcg cgggcctgca gccgctcctg gtcggctgct ccgaccgaac
    12301 cgtcggccag caggcgcttg aagcgggcgg catcgctggc cgcctggttc gactcgacct
    12361 cagcggcgcg gagctgctcg gcggcagctt cgaccgccag ttcgagatcg gcgggatcga
    12421 gccgcgcgag cggttggccg gcgcgcacgc gctgaccgac atcgaccagc ctggacaaca
    12481 ccttgccgcc ggttcgaaac gcgatctcgc tttcgacgcg cggtgccagc gtcgccgtgt
    12541 aacggcgttc gccggctgcg tgttccagtc gcacgggcgt gacgtagacc gcctgcgcga
    12601 ccggcgtggg agccactgct tccgagcagc cgacgagcag cgcagcgacg ctgctcgcag
    12661 ccagggcaac caggccaccg cgcggagcat ggggagtcgg tagtcgattc attgcggact
    12721 cgctgaaatg ggccggccgc cagcggcagc cgcgtccttc gtatcgggca tgcgccagcc
    12781 gccgccgagc gccttgtaga gctgtatggc atcaagtgcc agctgtgtcc ggtgttccgt
    12841 gcgtgccagt tctgcagaga tcacgccgcg ctgcgcatcc agcaactgca gcaggtcgat
    12901 ctggccttca gtgcgcagcg attcggcatg tcgaagcgcg gtgcggctct gcgactgcag
    12961 cgcatcgagc gcgtgcgccc gctcgcgttc ctgggccagg gccaccaggc tgtcctcgac
    13021 ctcctgcacc gcaccgagca ccgcacgctc gtactgcatc gcgctgccgc gctcgcgggc
    13081 cgtggcacgg tcctcggccg cgcgcagtcg cccggcgttg aacaacggca tcgaaaaagc
    13141 gagcgcgacg ttgctgtagc gcaccggcga aagcgcttgc gagttgacgg tcagatcctg
    13201 tgcgccgaac aaggcggcga ggaagaaccg cggaagcaga tcggcgcgcg cttcacggac
    13261 ccgagccccc tcggcggcaa gctggcgttc ggctgcgcgc agatcgggtc gccgcgccag
    13321 caactccgcg ggctgtcccg gcggcagggc gggcacctcc ggcagccggg gtgcgacccg
    13381 ctcgtcgagc atcggcaacg gttgcgtcgc actgcgcccg agtagcagtg aaatctggtg
    13441 cccggtggtc gcgaccagcg tgcgcagcgg gggcaattgc gcggcaagcg actgcgtctc
    13501 ggctgcggca cgggccacgt cgaaccggct cgccagtccc tgggcctcgc gattgcggat
    13561 caggcgctcg gtgtcgagtc gcatctgcag cagttcctcg aactgctgct gtcgcagtcg
    13621 cgctccctgc caggcgaagt acagccgggc gacttcgctg gaggccagca gcctggcgcc
    13681 ttcggctgct tcggcagaag cctgcgcgtc gaagtcggcc gcgtctgccg atgcccgggc
    13741 ggcaccgaag acatcgatct cccacccgag gtcgagcgag acgcgcgttg cacgggtgtc
    13801 cggcgaggtg cgcttgtagg cgtcgggcag accgctgcgc tggtcggaga cgctgccgtt
    13861 cagtccgagt gtgggcgcca gtcgagaagc tgcagcggtg ctgcctgcgc gggcctgcag
    13921 cacgcgcgtg gcggcgatgc gcacatcgag gttcgcctgc tgcgcttcgg tgacgagcgc
    13981 ggacagcacc ggatcgttga aggctgccca ccagttcggg tcgaacgagc cggcatcgct
    14041 gccctgatgg cggaactcgg ctgtctgcac cgcgttgcgg gcggaacggg tatccgggcc
    14101 atcgaccggc gaagcgcagg cagcggcgag caccacgatg cagagacacg cggcaaaccg
    14161 caccgttcga tgcagcccgg cgcgggccga tgcgcattgc gcggccggga agggatcagg
    14221 cctggcgggc ggagatcttc tgttcatggg caacggcttt cgggtcgatc cggacctgcg
    14281 acaagggagc ggagttgaca atgtcaacca tgatctcaag ctttgttgac agtgtcaaca
    14341 gctgggttcg catccgacat catcgatgat cggtccggtt gccctgcaac gcgagtcaga
    14401 cgggacggtc ccgtgctagg gtgcgtgccg gaagcgtgaa cagtccctgc gacgatgccc
    14461 aggaaaatcg aagaaccgat cgagctgaag gaggcctgcg tgcgtgcggc acgcgaggtc
    14521 attgccgagc acggcatcga gcagctcagc ctgcgcgagg tcgcgcgcaa gctcggggtc
    14581 tcgcatcagg cgccctacag gcactacccc agccgcgatc atctgctggc cgaggtgatg
    14641 cggcgctgct tcgagagttt cgcccgctcg ctcgacgagc gacagcgcag cggcgagggc
    14701 gccatctgcg acctggagtc gctggggcga cagtacctgg cctatgccgc cgcgcacccg
    14761 ctcgaatacc ggctgatgtt cggcaccccc tggccggaac ctgcggagca cccggaactc
    14821 gtgcacgacg cggtgcatgc cttcgacgtg ctgcgtggcg tgctgcgcag cctctacggt
    14881 gatggcgccg aaatgcgctc gcaggtcgac ctgcaggcga tgttcatctg gtccacgatg
    14941 cacgggctcg cgagcatcat gcagtcgaat gcaatggatt gcctgacgct tgcgcccggc
    15001 gcaaaggcgc aggcccccga atacgtgatg gacatgatca ggctggccct cgggccctgt
    15061 cgttgagcgc gaagcagggg gtctggggcc ggcggatggg ctgaccgccg gtggtgatca
    15121 ccggcggtgc ccgaggctga tcttcgccag tggatcagtg atcgtcggaa tgatggtcgc
    15181 gatcgcgacc cttcgacaga tcgatctcga acagcgaggt ggtgtcgctg acctcgtgcg
    15241 ctgctgcgac gaagtagcgc ttgcctactc ggaaggcctt caacccttcg ggcgacacgt
    15301 cattgagcac accgtcactg acgatcatgt cgatgaagcg ggcgtccctc gggttggtga
    15361 tgtcgtagac cgcgatcgcc gatttcagcg tgcgttccag accgatgaat gccagcaccc
    15421 gtccctcgat gtgcagcagc gccacgcctt cgggctcgac acccttgttg tcgctgcggc
    15481 catcgtcgta gatgccccgg gtgatcgctt cccgatcgag ctggctgccg ctgtcgaaca
    15541 cgatggttcc tgcggcgtcg cggatcgaga acgagtggcc tccgaaggtg accagcgcgc
    15601 ccgcggctga atcgacgttg gacagggtca gacgcgacag atcgttgcct tccggcacca
    15661 gttcggcggc gccactgccg gccgagccac gtcgctcgtc agcagaatcc tcgcgcgaat
    15721 cgccctcgtt ggccatcacc aggtaggtgc ggtccttgtg gctgtaggcc gcgatggtgt
    15781 cgggctggta gagacccttc accgccgccg ggcggagttc gatccgcccc ggaacgccag
    15841 agccgttttc ctgatcgtgg ggatcgatct cgttgcctgt caggctgaag tccttcaagc
    15901 cgaggctgta gatcttttcg aagctgtcgg tcttcaggtt cagcaccgcg atgccattgg
    15961 cttcctgaag gccgacgtag gcgtacctgc cttccttgtc gacggcgatg tactcaggct
    16021 ccgggccgta gggcgtcatg ttgggccggg tggtgccgtt gcccaatgcc ggaaactgcc
    16081 gcagcgtgtc gtatccgacg atcgccgggt cgatcggcag tgtcttgacg ctgcgcgact
    16141 tgacttcgac gatgctcacg gaacccgccg ggtcgacctg cgatcctgca tttggggtcg
    16201 cctcgctggc caccagcaca cgcttgccgt tcggcgtgaa agtcagcatg tcgggcagcg
    16261 agccgaccac cacgggctgg ccgctctgcg cacgggtgcg ggtctcgtag aacacgacaa
    16321 agcccggcag gctgcgatcg atgccgttct cgatggccag agcggccagg ccgtcatgga
    16381 tcgcgacgct gttgaccgca cccaggttgg tcacatcgat gtgcgccacc aactgtccgg
    16441 tcgagcggtc gagcacatcg acgccgacga cgccagccac ccacagcgtg tcggtgcggt
    16501 cgtcatacgc aacgatctcg gccttctggc cggcgacacc gccgagcgca tgggcgtatt
    16561 gccagacgcg gctgatctgg acggcggcgg tctgcgggtg cgcaacggca gccgtgctca
    16621 gtgcgctgac agtgaacgcc agcgcgcagg cacgcagcaa cggacgcagg gtgaagggca
    16681 agggcatcga aggactccat gggaaacggg aagtccagga ggttctgaca tcgatgtgtc
    16741 atcttcatga cacatgaagt gccaaggggt tctgtccccc tcggttcggg ggccgcattc
    16801 gatgcagcga gccgcgtcga tgaccgcaac ggtcgttcgc gtgtcaggcg ggtgtcatcg
    16861 cccagccaaa gcggcctgtg cctccatcct gtcgtgaggg gtggcccggt gctgccaccg
    16921 cacggcgaac catgaggcct ctcccaactg gctcgacaca tcgattcggc cacccatcga
    16981 ctcgacgagg cgcagcgaga cagtcaggcc cagaccggtg cccggcacgg gtccgttttc
    17041 ctgccctagt cgatcgaagg ggcttgaaca gccggccgag ccgttccttc gtcaagccgg
    17101 caacgttgtt ctgaaccgcc gttcacgcag ccgcgttccc ggctgctccg gcaccgatct
    17161 cgacctggcg tccctcacgg ccgtacttga tggcgttgct ggcccgtgcc gaattgcaga
    17221 acgagatgtc gcgcggtggc tgtgccgcat cgagaccggt gctcgacttg aaccaggtgc
    17281 ggcctgcgtc gaccagcccg atcagtgtga tgggtcgtcc ggtgacctgt gcctccagca
    17341 cggacgcgct tgatttctgc gctggatcaa gaagagcgcg cagcgacgat cctgcgttga
    17401 catggatcac gcgtcgatcc gttgcatggc ttcatgcttc gagggccgtc atcatctgcg
    17461 cctcctggcg ccggcccaat ttgacgagcc catgaccgat tccccatcga agttcccacg
    17521 ccgccgaaca ttcgcgctca tccttctttc cgtgctggtg atcgcgggtt tgtctgcgcg
    17581 ttggtggctc gggccccagg tggcggccga gaccgtgcag cggcgtgact tcgtgcagac
    17641 cgtggtggcc agcggccgcg tcgagacgcc acatcgtgtc gacatcggtg cccagatcac
    17701 aggcacggtg gtgcgggttc cggtcgccga gggccagagc gtgaaggcgg gtgacgtgct
    17761 gatcaccctc gagtcggccg aactgcatgc ggtggtgcgt caggccgagg tgtccgttgt
    17821 ccaggccgag gcacgtgtgc ggcagctgcg tgaacttcag gccccggtgg ccgagcaggc
    17881 attgcggcag acgcaagcca gtctcgacaa cgcccgctcg acgctgcgcc gcaaccagga
    17941 cttgttccag aaggggttca tcggcgaggc ttccttcgaa gactcgcgca aggcctatga
    18001 actggccgat gcgcaggttc ggtctgcgca gaaacagctc gatgccgctc gcccggcggg
    18061 cagcgatcat gcgcttgcag tcgccgctgt ggctcaggcc caggccagtg ccgaggcggc
    18121 gcgggctcgc gcggcctatg cggtcatcag cgcgccggtg tctggcacgc tgatcggccg
    18181 caacgtcgag gtgggtgacg tggtgcaggc cggcaaggtg ctgatgacgc tgtcgcccgc
    18241 cggccgcacc caactggtgg ctgaaataga cgagaagaac ctgcgcctgc tggcactcgg
    18301 ccagaaggcg gttgcatctg ccgacgccta cccgcagcag cgctttgatg ccgaactggc
    18361 ctacatcaat cccggcatca acgcgcagac tggcgcggtg caggtcaagc tcgacgtgcc
    18421 cgcgccgcca tcggtgctga cgcaggacat gacggtgtcg gtggatatcg aggtcgcgaa
    18481 gcggcccggc gcgttgctgg tgcccctcag tgcaatccac gatgccgacg gtcccgaggg
    18541 caccgaggcc tgggtgttgc gcatcgaggg cggccgggcg gtgcgaaagg tggtccgcct
    18601 gggcctgcgc agcgggggct gggccgagtt gctcgatggc ttgagcgaag gggatcgcgt
    18661 gttgccgtcg tcggcgtccg tgctcaccgg ctcgcgtgtg cgggcggctg catcctcggt
    18721 gaactgacgc tgcgcatgtt caccgcctgg gtgccgttcg agtggatcgt ggcgctgcgc
    18781 tttctgcgcg agggacgatt gcagacgctt ttcatcgtgg ctggcgtcgc cgtcggtgtc
    18841 ggcgtcatcg tgttcatgtc ggcgctgctg gcaggattgc aggccaactt catcaaccgt
    18901 gtgctgtcgg cgcaggcaca tatccagctg ctgccgccgc aggagatcac gcggccgttg
    18961 cgcggtgacc gtggtggcgc ggttttgcct ggcggcgagg ctgaaggcgc gatcgtgcag
    19021 ccgccgctcc agagactgaa gtccatcgat cagtggcggt cggttgccgc tcagatccgc
    19081 gcgatgcccg aagtgctggt ggtttcaccg gtagccggcg gttccgcgct ggcgatacgg
    19141 ggcaacgtga gtcgctcgat ctcgctcaac ggcatcgagc ccgagctgta tttccgcatc
    19201 gtcgatctcg gagcgaagat cgttagcgga agcgcccgcc tgaccggcac cgacatcctc
    19261 atcggcacgg agctcgccag tgacctcggc ctggacgttg gcgacaagtt gcgcgtcagc
    19321 accgcgagcg gcgccgccat cgcgctcacc atcactggcg tgttcgacct cggaaacaag
    19381 ggtgccaatg agcgaagcgt cttcgtggcg cttcacaccg ctcagagcct gctcggtctg
    19441 tccggtggcg tgtccagcct ggaggtgact gtgcgtgacg tctatgcggc cgaagcgatc
    19501 gcgcagcgca tcacggcctc gacgggcgtc gaagcggaca gctggatcaa gaccagcgcg
    19561 cagttcttcg ctgccatcag tgcgcagacc ctggccaaca ccgcgatccg attcttcgtc
    19621 ggcttgtcgg tggccttcgg cattgccagc gtgctggtgg tcgtggtggt ccagaagtct
    19681 cgagagatcg gcatcctgcg ggcgatgggc atctcgcagg gccagatcct gcgcgtgttc
    19741 cttcttcagg gggggctgct cgggctcggc ggtgccatgg tcggcagcgc gattggagcg
    19801 ggggccctga tcctgtggca gcgatacgca cgaaacgccg atggaacgcc gctgtttccg
    19861 ctggtattcg atccccatct cttcgcggcg gcgctgctgc tggcgatgct caccggcctg
    19921 gtagccgcct ttgcgccggc gttgcgtgcc gcccgtctcg acccggtggt ggcgattcgt
    19981 ggctgacacc tcgcttcttg ccgcgccagc gggtctggcg gcgcgctcga tcgtcgtgtc
    20041 tctgcacggt gtcaggaagg cgtacaacgc gggcacgccg atcgagaccg aggtgctgca
    20101 cggcatcgac ctgacgctgc tgcagggaga gttctgtgcg gtgatgggtc cgtcgggctc
    20161 gggcaagagc accttgctga acatcatcgg gctgctcgac cgaccgagcg ccggcaccgt
    20221 ggccgtctgc ggcgaggaaa ccacttcgct tgatgaccgc gctttgacgc ggctgcgcgg
    20281 ccattcgatc ggcttcgtct tccagtacca ccacctgctc accgccttca ctgctctgga
    20341 gaatgtgatg atgccgatgc tcggtgctgc aggcttcccg aacgatgcga tgcgtcagcg
    20401 agccatcgag ttgctggatg ccgtaggtct gaaagatcgt ctgcacgacg gtgctggcaa
    20461 cctcagcgga gggcagcagc agcgggtggc gatcgcgcgc gcgctggcga tgaacccggc
    20521 gctgctgctg gccgatgaac ccaccggcaa cctcgatacc cgcaccgccg gcgaggcgtt
    20581 ctccctgctg cgtcgcttca accaggagca cggtacgacg gtcctgttcg tcacccacaa
    20641 ccccgcgctg gccgagcgat gcgacaagac cattcaggtg gtcgacgggc tggtggacgg
    20701 atgatgtcgc cgtcagcgca gcgtgtagcc gcgcgcatcc atgcaggccg caacggcgcg
    20761 gttgaagacg tcggcgtcgt tcatcgcatt ctgctgggtg gttgcccagc ggttgcagtc
    20821 ctggcggtcc gagtcggtct gctgggtgct ctgtccgttg cggggataga tcaccggatc
    20881 gggcgctggc tttgcagcag cagccggcaa gggcgccggt tgcgcgttct cgatgcccgg
    20941 tggcggatcc accacggcat agccctgacc cggcgccagt gcgtagtaga cgccgttcgc
    21001 gtagtagtag ggtgccccgc cgatccacaa ggtggcgtag gcgggtggca gcagcggaac
    21061 gatcacaccg atcggcggcg caatcacgac gaagcgtccg ccaagcgccc ggaaccacac
    21121 gccgccatgg aaatagaagt ggcccccgcc gaagccgatg ctgatgctgc ctcctggcag
    21181 cgccgggatc acctcgccat gtcgcgggta gtagtgatcg tgtcgaaagc gctggtcgag
    21241 ccacaggtgc ggagcacggg tgttgcgaga aggcggcggc ttgcgaccgt cgtgtcgatc
    21301 cgcggcgacc gtcgggccta cggcaagcgc cgatacagca aggatcgcga cgatcgagca
    21361 agtcaggagt ttcatcatga tgagccccat gctgatgggt gtggatcgaa ctcggctcac
    21421 ctcggcatgc gcgccgagtt gccgctgaag atgcgcacat tgcaagcgat gggcacgtcg
    21481 cggcgattgc gcttgcgcaa ggaattgcgt attcgggtgc gcggtccggt tcctccgggt
    21541 cgatccgcgc ttcacctgcc ggctccacga tgatctcaac ctgatgggaa ccgactggag
    21601 aaaatggttc agtgagctcg cgcgacggcg ccgcacgctg cgccatttcg ggcgtcatcc
    21661 actgttggcc acgctggccg gccgcggtcc ggtggcttcc gtgcccgacc acgcggcctc
    21721 cgagttgctg ttggtcgccc gggccgagcc tgcggcagcc atcgctcggc tgcgttcgca
    21781 tgcggatggc ctgagcccgc gtgaagcagc ggcaaggctg cgccgccacg ggcccaacga
    21841 ggtcgcgcac gagaagccgc tgcctccctg gctgcatctg tggcactgct acaagaaccc
    21901 gttcaacctg ctgctgaccg cgctggccgc ggtgtcctgg ctgacacagg atgcgaaggc
    21961 caccgtggtc atcggggtga tggtcatcct gagcaccggc atccgcttcg tgcaggaagg
    22021 tcgttcgcat cgcgccgcgg atcgcctcaa ggcgatggtg agcaacaccg ccacggtgat
    22081 gcgccgcccg ccggatgggg cggacccggc ggcaccggtt gctgtgcctg caccccgcga
    22141 gattccgcag cgcgaactcg tgcccggcga cgtggtggcg ctgtctgcgg gtgacatgat
    22201 cccggccgat tgccgggtgc tgactgctcg ggacctgttc gtggcgcagt ctgctatgac
    22261 cggcgagtcg ttgccggtgg agaaattcgt ccagcacgaa ggggagggcg gcgggctgct
    22321 ggaacagggc aacctggtct tcatgggcac caacgtcgtg tccggggcgg cgacggcgct
    22381 ggtggtcgcc accggcaacc acacctgctt cggagcgctg gcgcagagcg catcggcgaa
    22441 cgaagccgag ccgaatgcgt tccatgcggg ggtgaacggc gtgagctggc tgctgatccg
    22501 ctttgcgatg gtgatggtgc cgatcgtgct ggtggtcagc gggttcgcgc agggggactg
    22561 gttgcaggcc ttcctctttg cgctgtcggt cgcggtcggg ctgacgcccg agatgcttcc
    22621 gatgatcgtg acctcgaccc tcgccaaggg ggcggtacag ctgtcgcgca agaaggtcgt
    22681 cgtcaagcgc ctggacgcca tccagaactt cggcgcgatg gacctgctgt gcaccgacaa
    22741 gaccggcacg ctgacgcagg accggatcgc gctggcgcgc aagagcgatc cgttcggcct
    22801 gccgtccgat gaagtgctga agtacgcgtt cctcaacagc tattaccaga cgggactgaa
    22861 gaacctgctc gaccatgcgg tgcttgaaca cgtcgaactg gccaccgagt tgaagctcaa
    22921 gcaggactac cggaagatcg acgaggtgcc gttcgatttc gagcgccggc gcatgtcggt
    22981 cgtggtctcg ctgaagtcag ccgaactcgg cgagcagcac cagctgatct gcaagggcgc
    23041 ggtcgaggaa gtgctggccg cctgcacccg gctgcgcatc gtcgacgcga cggggacacg
    23101 tgatgtcgcg ctcgacgcgg acatgctgtc gcgtgtgcag cgcgccgcac accagctcaa
    23161 cgacgagggc ctgcgggtgg tggccgtggc gatcaagacg ctgccggcgg agcagaccac
    23221 ctacgcgatt tccgacgagt ccgaactgac gctggtgggc tccatcgcct tcctcgatcc
    23281 cccgaaggat tccgccgccg ctgcactgaa ggccctgggc gcacacggca tccgcgtggt
    23341 cgtgctgacc ggcgacaacg atctggtggc cgcccgggta tgccaccagg tcggcctgga
    23401 cgcgtccacg gtgctgctcg gttcgcaggt cgaggcgatg gactccgcgc agctggcgaa
    23461 ggccgtcgag cagcacacgg ttttcgccaa gctctcgccg atgcacaagg aacgcatcgt
    23521 gcgggcgctg cgagccaacg gacacgtcgt tggcttcatg ggcgacggta tcaacgacgc
    23581 cccggcgctg catgccgccg acatcggcat ctcggtcgac accgcggtcg acatcgccaa
    23641 ggaagcagcc gacatcatcc ttctggagaa gagcctgctg gtactcgacg aaggcgtcgt
    23701 cgagggccgc acgaccttct gcaacatgct gaagtacatc cgcatgacgg cgagctcgaa
    23761 cttcggaaac gtgttctcgg tgcttgtggc aagcgcgttc ctgcccttcc tgccgatgct
    23821 gccgctgcag ctgcttgttc agaatctgct gtacgacgtc gcgcagaccg gcatcccgtt
    23881 cgacaacgtc gacaccgaac tcgtcggcca gccgctcaag tgggatgccg gcggcatcgg
    23941 caggttcatg ctcttcttcg ggccgatcag ttcggtgttc gacctgacca cgttcgccgt
    24001 gatgtggtgg gtgttcggtg ccgatacggt tgccgagcag gcgttgtttc acacgggctg
    24061 gttcgtcgtg ggcctgctga cgcagacgct ggtggtccac atgatccgca cgccgaagct
    24121 gccgttcatc cagagccttg cgtcggcgcc gctgcttgcg atgacggcgg tcgtgatggc
    24181 ggtgggggtc tggctcccca tggggcctct ggctgcccac ttcgggctgc agcccctgcc
    24241 gctggggtac tttccctggc tcgtcggtat cctgtccggc tactgcgttc tcaccacgct
    24301 gatgaagcgc ctctacatcc gacgttacgg ctggcaatga gccaggcgca cacgcccacc
    24361 cgtccggccc gaatgatcgc tgctgcgcag cgggcccgac acctcacgaa ggttcccgga
    24421 tgacctacat ggaatttgcg ggcgatccgt cgacgatgga ggccacgcag gtcgccagcg
    24481 cgcttcaggt cgacgtcgat cagggattga cgcaggcgga agcgtcgaag cgcctggcgc
    24541 agtgcggtcc gaacgcgttg cgagccacgg ccacgctgcc ggcctggcgc cgctggctgt
    24601 cgcacttcaa cgatccgctc gtgttcctgc tgctcggtgc ggcaggcgtg gcgttcgccg
    24661 catgggtcgt ggaagggcgc cacggtgccc cggtcgatgc gatcgtcatc gtgctggtgg
    24721 tcgtgctcaa cgccgtgctg ggctacctcc aggaagccaa ggcagccagc gccgtggctg
    24781 cgctggcgcg catgacggcc acgacctgca ccgtcctgcg cgatggccac caatcgcgca
    24841 ttccgagtgc cggcctggtg cccggcgacg tgatgctgct gactgaaggc gatgccgtcg
    24901 gcgccgacgc gaggctgatc caggtggcgt ccgcgcaact gatggaagct tccctgaccg
    24961 gcgaaagcca gtcggttctg aaggacgcgg ccacgctgcc agcgccggtg ccgttgggcg
    25021 atcgcctgaa catggtcttc aagggcacgg cggtggcgca gggcagcgcc cgcgcggtcg
    25081 tgacggccac cggcatgcac accgaggtag gctcggtcgc cgccctgatg gacgcgaccc
    25141 gggacgcgcc gaccccgctg cagcaggagg tcgcccgcat cggccgccgg ctcggcatgg
    25201 cggcgctcgt cgtggctgcg gtggtcatcg tcacgatcct gctggtgtcc gacatccgca
    25261 gtgcggccga tgtgatcgag gtgctgctgc tcggtgtctc gctggcggtc gcggccgtgc
    25321 ccgaggggct gcttgcgatc ctgtcggtgg tgctcgcgat gggcgtgcag cgtatggcgc
    25381 gcgagaacgc catcgtcaag aagctggcgt cggtcgagac gctcggctcc gcaacggtca
    25441 tcgccaccga caagacgggc acgttgaccc gtgccgagat gacgatcgtg cgcgtgatga
    25501 ccgcgtccgg cggcagccat gtcaccggcg tcggctacgc gccggagggg cgtgtcgagc
    25561 accagggtgc ggtcctgact gaaggcccgg tgcacgacga agcccggctg gtgttgagcg
    25621 gtggcagcct ggcgggcaat gccgatctgc gccaggccga cgatggtgcg tgggcgatcc
    25681 agggcgaccc gaccgaagcc gcctttctgg tcgccgaacg caagctcggc accgacgcgc
    25741 accgcaagcg gcgcttcacg cgcatggccg agatcgcttt cacgtcggac cgaaagatga
    25801 tgtcgacgat cgagaccgat cacgatcagg gcgatgcacc cgtgctgatc accaaggggg
    25861 ctccggacgt gctgctcgcc cgctgcacgc gagcccgtgt cggcaaggcg atcgtgccgc
    25921 tcgacgacgc gctgcgccag cgcgtgctgg ccgatgtcga cacgctgagc gacgctgcgc
    25981 tgcgcacgct ggcggtggcg taccgccccc tcgagccaga cgaatcaccg caggccgaag
    26041 gcctcgtggc gccatcgatc gaacgcgacc tgatctacgt cggcaccgtc ggcatcatcg
    26101 acccgccgcg caccgaggtc gctctggcga tcggcgaagc gcaccgtgcc ggcatccgtg
    26161 tcgtgatgat caccggcgac catccgcgca ccgcgctgcg catcgcgcac gatctcggca
    26221 tcgtcggcgg cgatgccagc gcgttgaccg gcaccgatct cgatgcgctg gacgatgcgg
    26281 cctggggcga agcggtgcgc accacgtcgg tcttcgcgcg cgtggcgccc aagcacaagc
    26341 tgcgcatcgt cgcggagctg caggccgccg gcaacgtcgt cgcgatgacg ggcgatgggg
    26401 tgaacgatgc gccggcgctg aaggccgcgg acatcggcat cgcgatgggg cgtaccggca
    26461 ccgaggtgac caaggaagct gccaacatga tcctggcgga cgacaacttc gagaccatcg
    26521 tcgtggccgt gaaggaaggg cgcggcatcc tcgacaacat caagaagttc ctgcgctacc
    26581 tgctgtcgtc gaacctcggc gaggtgctga cggtcttcct gggcgtggtc ggcgccggtg
    26641 tgctcgggct cgccggtgcg acgggcggcg tcgtgctgcc gctgctggcc acgcagatcc
    26701 tgtggatcaa cctcatcacc gatgccgggc ccgcgctggc gatgggcgcg gacccgatcg
    26761 ccggcgacgt gatggcccgt ccaccgcgca agcgaaccga acgggtcatc gatgcgcgca
    26821 tgtgggcagg cgtgctgcaa gtcggactgg tgatggccgt ggtgagcctg ctgacgatcg
    26881 acgcctacct gcccggcgga ctgatcgagg gcgcgcacga cctgacgctg gcgcgcaccg
    26941 ctggattcac cgtgctggtg tttgcgcaac tgttcaactg cctgaacgcg cgctcggagc
    27001 gtgccagcgc attcgaaggc atcttcgcca accgctggct ctgggggtcg atcctgctgt
    27061 cgacgctgct gcaggtggcc gtggttcacg tcggcgtgct caacgtcgcc ttcggaacca
    27121 cgccgctgtc gatcgagcaa tggctgggct gcgccgcgat ggccagcgtc gtgctgtggt
    27181 tctccgaggt ccgcaagtgg gtgcttcggg cagtgtcccg gcgcggcgaa ccggctcgcc
    27241 gctgaagcgg cggactcgcg cagcccgctg accacgccct cgcgtcagcg gggcagggtt
    27301 gacaggttct gcggcgtcga gatctcgcgg aatatgccgc cggtcgcggc gctgccgctg
    27361 gtgctgcctt gcccctgcat gcgcgccagg cacgccaggc gctgtgcttc tggcaacgga
    27421 tcgcagcgcc gaagtgcgtt gtcggtcaag gcggggctgc cgacttgcag ggcgccccga
    27481 cgtgcaagac aacgcacttc ggcgct
//
LOCUS       DAOORV010000036        27185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2100_length_27185_cov_10.470254, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000036 DAOORV010000000
VERSION     DAOORV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_27185_cov_10.470254"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKV56_06895"
     CDS             complement(<1..702)
                     /locus_tag="PKV56_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX67577.1"
                     /translation="MSRNDDRSPANSFDPYNADRPLMMKCGCGRDHSAADHQAEAQAL
                     SAEAHSRDFIEATLVKALFPHEPLRRRFLQAVGANTARAAIASLLPMGALEAMAQDKG
                     SLEKTDLKLGFIAITCATPLIMAGPLGFYKQQGLNVQLNKTAGWALIRDKMLNKEHDA
                     SHFLSPMPLAISMGIGSVAQPMRVATIQNINGQAITLALKHKDNRDPKGWKGFKFAVP
                     FEFSMHNFLLRYYLAE"
     gene            complement(823..1152)
                     /locus_tag="PKV56_06900"
     CDS             complement(823..1152)
                     /locus_tag="PKV56_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67578.1"
                     /translation="MNGAHGMLLDEPMRSLLQLRLDDLERRLVGMSQAMALMLRQPRS
                     ARDEAADRARPELVALAEVRAARARLEAGSYGLCATCDAPIEQHRLAGRPQVLQCSFC
                     EQQARRR"
     gene            complement(1332..2585)
                     /locus_tag="PKV56_06905"
     CDS             complement(1332..2585)
                     /locus_tag="PKV56_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOX67579.1"
                     /translation="MDIPQDGFVGLAVALGAGLLIGIERERRKGAGDDRQAAGLRSFT
                     VVAMTGALAQWLSVPGLVVCGAGLVAVLAAAAYWKSRSSDPGLTTEVALFTTYLIGVQ
                     CVLSPAFGGACGVGLATLLAARERLHRFATQLLSDQELHDGLLIAALGLIVLPLIPAG
                     PLSWLGGIDPRPLASMVLLLLGIQAAGHVALRWLGPRGGLLLSGLVSGFVSSTATVAS
                     FGSRARTHPQQMTMLAGGAALSSVATWLQALVMSSVLSPAAAIAIAPVALAGAVGGAA
                     AGLLSLRSSAARSAPDEVPAQEGGALRPREAIAIALLLAVVALVVGSAQRHFGDTGLN
                     VSIAIASLVDAHAPVASLASLHAAGSLTNHHMVLGVLIAITTNSISRCAVAAFAGGRK
                     YAARVSTALAAGLGAAWMMGLATGL"
     gene            complement(2588..3943)
                     /locus_tag="PKV56_06910"
     CDS             complement(2588..3943)
                     /locus_tag="PKV56_06910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX67580.1"
                     /translation="MKITFLGAADSVTGSKHLIDAAGARVLLDCGLFQGYKALRERNW
                     APLAVKPSEIDAVVLSHAHLDHCGWLPALVKQGFKGPVHASAATRDLAEVLLLDSAHL
                     QEEDARRSNRGGWTRHAPALPLYTVADAKRAIARIEVLPAGRSVRIGDARIDFTPAGH
                     LLGAVSVRLRARDRTLVFSGDLGRSDDLLMPPPGRVAQADVLLIESTYGNRVHPKEDV
                     QARLGEIIRATTRRGGSVLLPSFAVGRAQALLLVIQRLKAAGEIAADVPVYLDSPMAT
                     TATELYQRHRRLLRIPSREAGSLTDDVTLIAGPKESEKLTRMRWPKIVISAAGMATGG
                     RVLYHLKTLAPNPRNHIVFPGFQVGGTRGAHLIAGAREVKIHGEYVAVKAEVSHLEGF
                     SGHADADGLMAWLRGFEAPPERTFVVHGEPDAADTLRLRIQDELGWKVSVPQHGATVE
                     L"
     gene            complement(4018..4704)
                     /locus_tag="PKV56_06915"
     CDS             complement(4018..4704)
                     /locus_tag="PKV56_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67581.1"
                     /translation="MIRVLICDDHLIVRQGIKQILADTGDIVVAGEADTGPEAIRKVR
                     EASSADPGGEEIVGIDVILMDIAMPHRDGLDVLRQLKTEFPKLPVLMLSTYPDKQYAV
                     RSLKLGAAGYLNKSADSEQMSAAIRKVAAGGLFITPTVAEHMATALGAGVGSMRPVDE
                     PLHERLSHREYQVFRLLAGGRSVGEIAEQLVLSSNTVSTYRARILDKTGARNDVELAL
                     YAVKQELLHV"
     gene            complement(4701..5987)
                     /locus_tag="PKV56_06920"
     CDS             complement(4701..5987)
                     /locus_tag="PKV56_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOX67582.1"
                     /translation="MTFLVSPLPTRPSTVDASVSSDATGSVWSDQLRLLLESTGEGIF
                     CVDMFGICTFVNRAAAEMLGYPNDAVLGRNMHELMHHTHADGQPYPEADCPIFNAFRQ
                     GLPCRIDSEVLWRSDGSPFSAEYSSHPILDGGVVQGAVVTIVDITERKRSEALLQQSH
                     EQLERRVGERTAELTQALGALRELSAYSESVREEERTRIAREIHDELGSLLVALKMDV
                     GWVEKRVQDRPELSCKCHGMGRTIDTAVDNLGRIITDLRPSILDHQGLWAALEWQAQE
                     FIETTELASDLQMHVAAQTEPPSGPDGDRWSIAVFRIFQEMLSNVARHAQARSVRIRL
                     YVDGPPHPELYIEVRDDGIGAEPSALEHPRSYGVMGMRERAGHFGGSLAIDSVPNHGT
                     CVRLTMPMPERDGDGLTASSDRPPGTANTAFVKGAP"
     gene            6208..6978
                     /locus_tag="PKV56_06925"
     CDS             6208..6978
                     /locus_tag="PKV56_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HOX67583.1"
                     /translation="MSKPQTPPAKHISRDLTDAEFSELDDLLAETPEPFEPVDVVMLD
                     GFLCGVLVQPVLLDSATWLPHVFDFDGRPLPDDVDPAWLERITALVLRRHAALTRAIA
                     EDGWFDPLVLEFDEDHPAEPPADGGPDPLAGLHPVSQSLMPWVAGFQHATLCFPDLSE
                     MTDDAVLSALARLYRHLPAETDDEREVVATLDREHPLTTLEDGIEDIVIAVADLSDLT
                     RKARFKVETVRRDVPKAGRNDPCPCGSGKKFKACHGKT"
     gene            7114..7896
                     /locus_tag="PKV56_06930"
     CDS             7114..7896
                     /locus_tag="PKV56_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX67584.1"
                     /translation="MRLQLLSDLHLETEVFEPRPAPGAELLVLAGDIDSTWRELDRFG
                     GWPVPVLFVAGNHEFDQRELDDAWPALRERCDALGVKLMERQSCVVTDSTGRRIRFVA
                     TTRWSDFDLFGAAQRDRSMRAATYFVDVMRSTLHGALFDAAAVRVESLDCRAWLADEL
                     ARPPESACDAWDATVVVTHYGPSMRSADKRYGKQPGTASFCNADEDLMPRADLWIHGH
                     LHCRHDYRVAHAEGETRVVCNARGHSRKGEADHYDGLFTLEV"
     gene            complement(7913..8524)
                     /locus_tag="PKV56_06935"
     CDS             complement(7913..8524)
                     /locus_tag="PKV56_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX67585.1"
                     /translation="MARTSFKEQMLRAREDAIVSSVNRLLADKGYDVMTVDEVAADVG
                     IAKASLYKHFPSKEALAAAAMIRMLDRALAFIATLDHHPTARAIDHLKAVASWTMKVQ
                     LAGEMPSLPSQNSSLRSALTSNEAYLQRLMDVSEKLGAWIVAAQEAGHLNRQLPPEIV
                     LYTLFARACDPVLGLLKLSGQHSEERIIELLLVTCFDGLNSRP"
     gene            complement(8555..8689)
                     /locus_tag="PKV56_06940"
     CDS             complement(8555..8689)
                     /locus_tag="PKV56_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67586.1"
                     /translation="MGTAFGLDAYLQADEEFKAFQDSKRASSDAAVESVDRLNGRSVI
                     "
     gene            8985..10289
                     /locus_tag="PKV56_06945"
     CDS             8985..10289
                     /locus_tag="PKV56_06945"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HOX67587.1"
                     /translation="MKVIVMGAGIVGVSTAWYLLDEGHEVTVIDRQPDAALETSFANG
                     GQISVSFCEPWANADAPFKVAKWLLRDDAPLLFRPRLDPHQWRWGLSFLGQCNTAAFE
                     RNVSQLIALGRYSQDSLRTLVARTGIDYHRLERGILHFFSSERDFDAGAAAALVMRRH
                     GVDRQVLSRDEVLTVEPALAAFAPRLAGGTFTPSDESGDARVFTQQLARRCAERGAVF
                     LFGHDICDLQKSNNAIESVRVREQLTAQVKLLRADGYVAAMGSHTAPLLRPLGLCLNI
                     YPTKGYSAMLKLKQPERASVVSLIDDDRKIAISRLGDEVRIAGTAEMAGYDTSITSAT
                     SRVRCAALIRRYEELFPGVADTARPNYWAGLRPSTPDNIPIIGRSPIGRLWINAGHGT
                     LGWTHGAGSGRALAELISQKQPTLDFGFAGGAARPGPAPAHA"
     gene            complement(10302..10994)
                     /locus_tag="PKV56_06950"
     CDS             complement(10302..10994)
                     /locus_tag="PKV56_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HOX67588.1"
                     /translation="MNSTHRPRPASQPLDEHDIDELQVLLDRVPAPLEPLDVSMLDGF
                     LCGVLLQPQPVPPAQWLPFVTDADGRALPASFDAQRLHALALRRHAELNDAITQRQWF
                     DPWVFELESDEDESDADDLPHETDAVYPWVAGFATAQEFFPGLMRLEAKALTEPLALL
                     YRHLEADDLEDADDLIEEIESLEPAADLSAAVEELVRATLLLADISRPRPDATRPNRP
                     PARGPARRPQRR"
     gene            complement(11023..11961)
                     /locus_tag="PKV56_06955"
     CDS             complement(11023..11961)
                     /locus_tag="PKV56_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX67589.1"
                     /translation="MARPDALWPHVHLLTVLAQTGSFTQAAQRLALSKAAVSQRLSEL
                     ERAVGVPLVQRTTRSVRLTDAGRQLADETSGAFEQITQSLGSVRDLAGQPRGLVRLTA
                     PVALGRQQVAPSLEGFFKRYPEVRIELELSDRLLNLAHEGFDLAVRHTFAPPDNHVAW
                     KLRDSRTLLVASAGYLRRRGTPLHPSDLASHACLGYLRPGPTVWRFEALPVAAGASDT
                     QRISVGVQGPLCANNSEVLRDAVLAGLGIAQVPDFSAAAALRAGRLREVLPAWRPVGY
                     FGDAIYAMRPWSPITPRAVRVLVEHLQAEFSRGPAA"
     gene            12092..13624
                     /locus_tag="PKV56_06960"
     CDS             12092..13624
                     /locus_tag="PKV56_06960"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOX67590.1"
                     /translation="MGAPDAMTRTYASTAELGHFIDGHAVASTSGRRQAVYNPATGAV
                     ARQVALASAGEVNVAVAAAQAAFPAWADTPPIRRARVMFKFLELVNLHRDELAAMITA
                     EHGKVFTDAQGEVSRGIDIIEFACGIPQLLKGDYTEQVSTGIDNWTLRQPLGVVAGVT
                     PFNFPVMVPCWMFPVAIAAGNTFVLKPSERDPSPSLFMAELLREAGLPDGVFNVVQGD
                     KLAVDTLLSHPDVKALSFVGSTPIAQYIYETGAHHGKRVQALGGAKNHMVVMPDADLE
                     QTVDALIGAGYGSAGERCMAISVAVAVGDVADRLVPALAARARTLKIRNGMDLDAEMG
                     PIVTRQALERIEGYIVTGVAEGATLVVDGRGYKVPGHENGFFTGGTLFDHVTPGMRIY
                     KEEIFGPVLAVVRVPDFAAAVQLVNDHEFGNGVACFTSDGNVAREFARRIQVGMVGIN
                     VPIPVPMAWHGFGGWKKSLFGDMHAYGEEGVRFYTKQKSVMQRWPASIAKGAEFVMPT
                     AK"
     gene            complement(13652..14776)
                     /locus_tag="PKV56_06965"
     CDS             complement(13652..14776)
                     /locus_tag="PKV56_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOX67591.1"
                     /translation="MPRVMLRGVLIQTTRAAVVTSALWLVGCASAPPGSSPATSQDPP
                     VAASPAPAPAAERDGPPVAPPPNLADVPDAEPRIDPIRPGGPNKPYEVAGQRYEPLAA
                     DVAISQRGLASWYGRKFHGRSTASGERYDMYAMTAAHPTLPIPSYARVRNPANNREVV
                     VRINDRGPFHSTRIIDLSYTAALKLDLLRGVGMVEVERLTHDAIRTGSWRQGSAARSV
                     ATAPAPGPASTAAIAAAPSSRDTATTAASTSPVQSQAGIVATPLPAAGEAKTEGGSPV
                     TREAGSVPDVTAQAPATPPPASGFWVQLGAFKQRERADRLHQRLALDLSWLAPLLGVF
                     SDQQLLRVQAGPYASRDEALRVAQRVRATAAMSPLVTERR"
     gene            complement(14778..15593)
                     /gene="hutG"
                     /locus_tag="PKV56_06970"
     CDS             complement(14778..15593)
                     /gene="hutG"
                     /locus_tag="PKV56_06970"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HOX67592.1"
                     /translation="MTTPEVIPPYRLVRGTRPMLLSIPHVGTHIPEALRGDYTDIALE
                     LADTDWHLDRLYAFAQDLGASVLQATHSRNVIDLNRPPDNQSLYPGQVTTGLCPTETF
                     RGEPLYRPGRAPDAVETARRLDTCWRPYHEALTIELARLKALHGCALLWDAHSIASYL
                     PRLFDGKLPDLNFGTAGGTSCAASILERVTARAQGSGCSSVINGRFKGGHITRHYGHP
                     AQHVHAIQLELCQSLYMNEASPFEYRPEVAARLQPTLKAMLEAAADAVMAPGH"
     gene            complement(15656..16651)
                     /locus_tag="PKV56_06975"
     CDS             complement(15656..16651)
                     /locus_tag="PKV56_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX67593.1"
                     /translation="MTLARPLDLHALEAFVACCDCQSMTQAALRLGITQSAVSQLVKN
                     LERDTGTLLLDRDLRPPKPTAAGRALREEATELLAHARAVADRARSGAPTEGTRLLLG
                     CVDSFAATVGPALIRALSGRARDLLMWSGLTPVLAEQLLRRDIDMAIVTDAVVADRRI
                     SVRPLFSEAFVLVLPPASENESGNPPDMQAAAARWPLIRYSLRSVMGQQVERCLRHFG
                     VQAPRRFEFDATDPLMSLVAAGLGWAITTPLCLLQSRHHLGDVRVLPLPATALGRREF
                     FLLCREGEWQGLDDEIVRLTQQVITQHTRADLERLIPALPSSALQVSPSKRSDPP"
     gene            16794..17978
                     /locus_tag="PKV56_06980"
     CDS             16794..17978
                     /locus_tag="PKV56_06980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOX67594.1"
                     /translation="MQTYDVIVIGAGVIGASVAYHLARLGAKRVLVLDRTQIGAGTTS
                     QSSGILRTHYSVRENVAMAQDSWKVFTDFANYLGDEDAASGLVKCGYLIASPDGPKLK
                     PLAAALEGQRQQGIEVRLLDRQQAEALLPIADLHDAALIGYEPEAGFADAYLVATSFA
                     RAARRGGVKIIEGVEVQQLLVEQGQVVGVATDQGRFLAGTVISTQNIWATQIEQWTGI
                     TTPVVSERHTVLALEGPQPYTFAMPVFKDLGSEGMLYCRSYGGTQMLVSEGTAGEVLA
                     GPDNEQGDVSLDTIASVGAQAAERFPSFETAGLASSWTGVYDVTPDWNPVLGRLPGIG
                     GLVVGYGFSGHGFKLSPAVGRVLAQEALGLTPDISLAPYALERFAAGRPLVGQYGVGA
                     VS"
     gene            17978..18619
                     /locus_tag="PKV56_06985"
     CDS             17978..18619
                     /locus_tag="PKV56_06985"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOX67595.1"
                     /translation="MALRAFRLDALPEEPWRNGGGRTRTIATRMRDADAAGSAAQDEG
                     PPWDWRISVATIERGGPFSSFPGVDRSSMLLDAGRIELSAAGEPTLLMRSPGDVVRYR
                     GDSVTTSAVHGASQPLSVLNVMTRRSAAQAWMKALRDDSYLAAQGLALLVLDGSWRVV
                     DGSMGAEDPDHGLTLHSGEGAWCTVSAPGSRAIWRVERLSPTGMLAAIELDRS"
     gene            complement(18752..19954)
                     /locus_tag="PKV56_06990"
     CDS             complement(18752..19954)
                     /locus_tag="PKV56_06990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOX67596.1"
                     /translation="MFEHIDPYAGDPILTLNETFGRDPRPHKINLSIGIYFDDAGKLP
                     VMGAVREAESAMLQTIGARPYQPMEGAVNYRQAVQHLLFGATHEAVTSGRIATVQTIG
                     GSGGLKVGGDFLKRYFPDSQVWVSDPTWDNHRAMFEGAGFTVNTYPYYDGATGGLRFD
                     AMLAAFKALPEHSIVLMHACCHNPTGVDLKAAQWAELIPVIRARKLIPYVDIAYQGFG
                     DGIDEDAFAIRALADAGIAFFCASSFSKSFSLYGERCGGLSVVCPNAAEAELVLGQLR
                     ATVRKNYSSPPTHGGQIVARVLQTPALRAKWAAELDAMRDRIRAMRHRLHAVLSAKLP
                     GRDFDYFITQRGMFSYTGLSPQQADRLREEHAVYVLRSGRICVAGLNSANVEATAVAM
                     ATVLSHPD"
     gene            complement(19998..20495)
                     /locus_tag="PKV56_06995"
     CDS             complement(19998..20495)
                     /locus_tag="PKV56_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOX67597.1"
                     /translation="MNASARSAFDFEATALDGTTVPLSAQRGCVLLIVNTASACGFTP
                     QFAGLEDLWKTYGERGLTVIGFPCNQFGSQDPGSNPEIGAFCQKNYGVSFPMMSKIEV
                     NGSSAHPLWTWLCAEAPGLLGTKAVKWNFTKFLVGRDGQVLKRYAPTDTPASLKADIE
                     AALSA"
     gene            complement(20593..21498)
                     /locus_tag="PKV56_07000"
     CDS             complement(20593..21498)
                     /locus_tag="PKV56_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX67598.1"
                     /translation="MASQLAGRAALALVFNALVWGLSWWPLRQLDAQGVHPLWATVLI
                     YAPTVLVIAAWRPRAVGQLLTQWPLWVLALAAGLTNAAFNWGTVIGDVVRVVLLFYLM
                     PLWSVLLARVLLKEPVTLAAGARIALAVLGAGIVLRPEGATGWAVLPFPQSLADGLGL
                     LGGFTFALNNVMLKREADRPEEGRALAMFVGGAVVAGALAAALSALGRVPWPLAGGVA
                     ALPWWAIALALTMSVLFLCSNLALQFGASRLPIHVTSVLMLSEVLVAAGSAALLGAGR
                     ITVPLLLGGAMIVAAAVWATRDGAH"
     gene            complement(21506..22783)
                     /gene="lplT"
                     /locus_tag="PKV56_07005"
     CDS             complement(21506..22783)
                     /gene="lplT"
                     /locus_tag="PKV56_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOX67599.1"
                     /translation="MKKGFYTIMAAQFFSSLADNALFVAAVELLKSSGQPEWQRAALV
                     PMFALFYVLLAPFVGAIADAVPKGRVMFFSNLIKVVGCLMMLFGSHPLMAYAIVGLGA
                     AAYSPAKYGILTELLPNSQLVKANGWIEGLTILSIILGVLLGGQLVGHRISEFLLAFD
                     FPLIDTGIDSPAEAAIASMVLLYALAAAFNLRIPRTEAPLQPIAHSLIGLVRDFARCN
                     ARLWQDKLGQISLSTTTLFWGVSGNLRYIVLAWASVALGYDTTLASSLVGVVAIGTAA
                     GAVAASLRMRLDQATAVIPLGITMGLLVIGMNFITNVWIAAPFLVLLGALGGFLVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQACILGLGALYAGMTKLGLSAFGAITIFGVIVA
                     GLMWLIRRWHQRNCIHHRVEVDHLLALARSDQH"
     gene            22940..24055
                     /gene="alr"
                     /locus_tag="PKV56_07010"
     CDS             22940..24055
                     /gene="alr"
                     /locus_tag="PKV56_07010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOX67600.1"
                     /translation="MPRPIRAVIHTEALAHNLRRIRAQAPDARVWAVVKANAYGHGIE
                     RAFPGLLGADGFALLDLDEAQRVRSLGWRGPVLLLEGCFEARDLELCSRLNLWHTVHR
                     EAQIDWLAVHKTQQPHQVFLKMNSGMNRLGFTPAAYRSAWLRLSGLTQVESITLMTHF
                     ADAEQAGDLHGRRAIDRQLAVFEAATQGLSGDRTLCNSAATLRHAPDDAAVRADWIRP
                     GIALYGSAPDFPQCSISDWKLRPTMTLRSELIATQDLQPGDTVGYGSCYNAERPMRIG
                     VVACGYADGYPRSAPTGTPVLVDGVRAPLIGRVSMDMVTVDLGPVPAARIGSEVTLWG
                     CGPGDSVLPIDEVANAAGTVGYELMCALAPRVPTHPG"
     gene            24052..24828
                     /locus_tag="PKV56_07015"
     CDS             24052..24828
                     /locus_tag="PKV56_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67601.1"
                     /translation="MKGSHRSAAGFERRVRADLKARRWLRWHAFLLGLLCFATLWSVS
                     AGLMHAGIETLALRWPLAFAFAYAAFIGLLWLWCRWLLSHDEFDADLNIDWNLDLPRL
                     DLGRGGAPFRAGGGGDFGGGGASASFDGLPGADAASEVAQTALDAVASSDEGVVVAVP
                     LAVVVGIALLIAGAIGFAVFGLFGIDVLLGVAVEIAFASAGGALAFRAQREGWLAHAL
                     SRTVWPAVALLLIFAVLGLVIDRWLPQAHSLPQALRLLHG"
     gene            24839..25246
                     /gene="arfB"
                     /locus_tag="PKV56_07020"
     CDS             24839..25246
                     /gene="arfB"
                     /locus_tag="PKV56_07020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOX67602.1"
                     /translation="MIRHFEVPPGEVEITAIRAQGAGGQNVNKVSSAVHLRFDVHASS
                     LPDDVKQRLLSLRDQRITAEGVVVIKAQTSRSQDANRADALARLQELVDSVTTPPRVR
                     RATKPTYGSKQRRLEGKSIRAQVKASRGKIGHE"
     gene            25278..26648
                     /gene="radA"
                     /locus_tag="PKV56_07025"
     CDS             25278..26648
                     /gene="radA"
                     /locus_tag="PKV56_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOX67603.1"
                     /translation="MSKPKSLYTCSECGGTSPKWLGRCPSCEAWNTLVESVAESTGPK
                     HRYQSLAGALPVATLADIEASDVDRLPTGLDELDRVLGGGIVAGGVVLIGGDPGIGKS
                     TLLLQALDALSRNVRTLYVTGEESGAQVALRSRRLGLGEATGGSQVRVVAEIQLEKIA
                     ATIAAEQPVVCVIDSIQTVYSDQLTSAPGSVAQVRECAAQLTRIAKSSGTTIVLVGHV
                     TKEGALAGPRVLEHIVDTVLYFEGDTHSSYRLVRAIKNRFGAVNEIGVFAMTERGLKG
                     VANPSAIFLSTHGDPVAGSCVLVTLEGTRPLLVEIQALVDSGGPSPRRLSVGLDRDRL
                     AMMLAVLHRHAGIACYDQDVFVNAVGGVRIAEPAADLAVMLAIQSSLRGKPLPRGFFA
                     FGEVGLAGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPRKPIEGLTIHAVERIEEAI
                     DRVRAL"
     gene            26720..>27185
                     /locus_tag="PKV56_07030"
     CDS             26720..>27185
                     /locus_tag="PKV56_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOX67604.1"
                     /translation="MSMADRDGKIWMDGELVEWRDAKIHVLSHTLHYGCGAFEGVRAY
                     KTQQGTAIFRLREHTERLFNSAKILRMKIPFSIEQVMEAQRRVVLENKLESGYIRPLT
                     WIGDKKLGVSPKGNTIHLMVAAWTWGAYLGEDGMKRGIRVKTSSYTRHHVNIT"
BASE COUNT         4283 a         9060 c         9498 g         4344 t
ORIGIN      
        1 ctctgccagg tagtagcgca gcaggaagtt gtgcatcgag aactcgaacg gcaccgcgaa
       61 cttgaagccc ttccagccct tggggtcgcg gttgtccttg tgcttcagcg ccagcgtgat
      121 cgcctgcccg ttgatgttct ggatcgtcgc cacgcgcatc ggctgggcga ccgagccgat
      181 gcccatcgag atggccagcg gcatcggcga caggaagtgg gaggcgtcgt gctccttgtt
      241 cagcatcttg tcgcggatca gcgcccagcc cgcggtcttg ttgagctgga cgttcaggcc
      301 ctgctgcttg tagaagccga gcgggccggc catgatcagc ggcgtggcgc aggtgatcgc
      361 gatgaagccg agcttcaggt cggtcttctc cagcgaaccc ttgtcctgcg ccatggcttc
      421 gagagcgccc atcggcagca gcgaggcgat cgctgcgcgc gcggtgttcg cgccgaccgc
      481 ctggaggaat cgacggcgca gcggctcgtg cgggaacagc gccttgacca gcgtcgcctc
      541 gatgaaatcg cgcgaatggg cttcggccga cagtgcctgc gcctcggcct ggtggtcggc
      601 ggcgctgtgg tctcgaccgc agccgcactt catcatcagc ggccggtcgg cgttgtaggg
      661 gtcgaaactg ttggcgggcg atcgatcgtc gttgcggctc atgcgaacac tccttagtga
      721 tgactggagc gtacgcagca agcacaggcc gcgccatcga gcctgtggtg aaagccgtgt
      781 agtgctggca ctacacgcgg cggcgcctgg cgccgccagc aactagcgtc tgcgagcctg
      841 ctgttcgcag aagctgcact gcagcacctg tggccgaccg gccaggcgat gctgttcgat
      901 cggcgcgtcg cacgtggcgc acagcccgta gctgccggct tccagccgcg ccctggcagc
      961 gcgcacctcg gccagcgcca cgagctccgg gcgcgcgcgg tccgcggcct cgtcccgggc
     1021 actccggggt tgccgcagca tcagcgccat ggcctgcgac atgcctacca gccgtctttc
     1081 gaggtcgtcc agccgcaact gcagcaggct gcgcatgggt tcgtccagca gcatcccatg
     1141 tgcgccgttc atggccgtgt gaccagcggt ccagtgtgat tgttgcgtct gcatgcgggc
     1201 ctccggatcc accccgcgcg acgtgcaccg ggctggggcc actgtagtga acgatgtgct
     1261 cgcggatctt gagcggcgtc aagtcgggga cggctcggcc tcacgcgtca ccccggatgg
     1321 aggcggcctc gtcacagacc ggtggccaac cccatcatcc aggcggcgcc caggccggcc
     1381 gccagggccg tgctcacccg cgcggcgtac ttgcgcccgc cggcgaacgc tgccaccgca
     1441 catcgcgaga tcgaattggt ggtgatggcg atcaggacac cgagcaccat gtggtggttc
     1501 gtcaggctgc cggccgcatg aagcgaggcc agcgatgcga ccggtgcgtg cgcatcgacc
     1561 agcgaggcga tggcgatgct gacattcagc ccggtgtctc cgaagtggcg ctgcgcgctg
     1621 ccaaccacaa gcgccaccac ggcgagcagc agcgcgatcg ctatcgcctc gcgagggcgc
     1681 agggcgccac cctcctgggc aggcacctcg tcgggagccg accgtgcagc actcgatcgc
     1741 agtgaaagca ggccggcggc cgcgccgccg acggcgcctg ccaacgcgac cggtgcgatc
     1801 gcgatggcag cggccggaga cagcaccgaa ctcatcacca gggcctgcaa ccaggtggcc
     1861 accgaggaca acgcggcgcc gcctgccagc atcgtcatct gctgcgggtg ggtgcgggcc
     1921 cggctgccga acgacgcgac cgtggcggtg ctcgagacga aacccgagac caggccggac
     1981 agcagcagcc cgcctcgcgg tccgagccag cgcagcgcga catggccggc cgcctggatg
     2041 cccagcagca acagaaccat cgacgcgagg ggccgcggat cgatgccgcc gagccaggac
     2101 agcgggccgg ccggaatcag cggcagcacg atcagcccca gggcggcgat cagcaggccg
     2161 tcgtgcagtt cctggtcgct cagcagctgc gtggcgaacc tgtgcagtcg ctccctggcg
     2221 gccagcagtg tcgccaggcc gacgccgcac gctccgccga aggccggcga cagcacgcac
     2281 tgcacgccga tcaggtaagt ggtgaacagg gccacttcgg tcgtcagtcc gggatcgctc
     2341 gaacgactct tccagtaagc ggcggctgcg agcaccgcga ccagacccgc gccgcagacc
     2401 accagacccg gaacggacag ccactgtgcc agtgcgccag tcattgccac cacggtgaag
     2461 ctgcgcagcc cggcggcctg ccggtcgtcg cctgccccct tgcgccgctc gcgttcgatg
     2521 ccgatcagca ggcctgcacc cagcgccacg gccaggccga cgaaaccgtc ctgcggaatg
     2581 tccacgatca caactcgacc gtcgctccat gctggggcac ggacaccttc cagcccagtt
     2641 cgtcctggat gcgcagccgc agggtgtcgg ccgcgtcggg ctccccgtgg acgacgaagg
     2701 tgcgctcggg gggcgcttcg aaaccgcgca accaggccat caggccgtcg gcgtcggcat
     2761 ggccggagaa gccttcgagg tggctgacct cggctttcac cgccacgtac tcgccgtgga
     2821 tcttgacctc ccgcgcgccg gcgatcaggt gcgcgccgcg cgtgccgccg acctggaatc
     2881 cgggaaacac gatgtggttg cgcgggttgg gcgccagcgt cttcaggtga tacaggacgc
     2941 gcccgccggt cgccatgccg gcggccgaga tcacgatctt gggccagcgc atgcgggtca
     3001 gcttctcgga ctctttcggt ccggcgatca gcgtcacgtc gtcggtcagc gagccggcct
     3061 cgcgcgacgg gatgcgcagc agccggcgat gccgctggta cagctcggtg gcggttgtcg
     3121 ccatcgggct gtcgaggtag accggcacgt cggccgcgat ctcgccggcc gccttcagcc
     3181 gctggatcac cagcagcagc gcctgtgccc gaccgaccgc gaacgacggc agcagcacgc
     3241 tgcccccgcg tcgagtcgtc gcccggatga tctcgccgag gcgcgcctgc acgtcctcct
     3301 ttggatgaac ccggttgccg taggttgatt cgatcagcag cacatcggcc tgcgcgacgc
     3361 ggcccggtgg cggcatcagc aggtcgtcgc tgcgtcccag atcgcccgaa aacaccagcg
     3421 tgcggtcgcg ggcgcgcagc cgcaccgaca ccgcaccgag caggtggccg gctggcgtga
     3481 agtcgatgcg cgcgtctccg atgcgaacgc tgcggccggc cggcagcacc tcgatgcgcg
     3541 cgatggcgcg cttagcatcg gcgacggtgt agagcggcaa tgccggtgca tggcgcgtcc
     3601 agccaccgcg gttcgagcgc cgcgcgtcct cttcctgcag gtgcgcactg tcgagcaaca
     3661 gcacttcagc caggtcacgc gttgccgcgg aagcgtgcac cggccccttg aatccctgct
     3721 tcacgagcgc cggcagccag ccgcagtggt cgaggtgcgc gtggctcagg accaccgcgt
     3781 cgatctcgga cggtttgaca gccagtggcg cccagttgcg ttcgcgcaag gccttgtagc
     3841 cctggaacag gccgcagtcg agcagcacgc gggcaccggc ggcgtcgatc aggtgcttgg
     3901 aaccggtgac gctgtcggct gcgccgagga aggtgatctt catgggaagg tggcatggcc
     3961 cgatcggtgg cggtcaccga ctttcgcaca cgtggagtca ccgctccccg accggcctca
     4021 gacgtgcagc aattcctgct tcaccgcgta cagcgccagc tcgacatcgt tgcgtgcgcc
     4081 ggtcttgtcg aggatgcggg cgcggtaggt gctgacggtg ttggaagaca ggaccagctg
     4141 ctctgcgatc tcgccgacgc tgcggccacc ggcgagcagg cggaacacct ggtattcgcg
     4201 gtgcgacagc cgctcgtgca aaggctcgtc gaccggacgc atggaaccga caccggcgcc
     4261 cagcgcggtc gccatgtgct cggccaccgt cggcgtgatg aacagtccac ccgccgcgac
     4321 cttgcggatg gcggcggaca tctgctccga gtccgcgctc ttgttcaggt agccggcagc
     4381 gcccagcttc aggctgcgca cggcgtactg cttgtcgggg taggtgctca gcatcagcac
     4441 cggcagcttc gggaattcgg tcttcaactg ccgaagcacg tcgaggccat cgcgatgcgg
     4501 catcgcgatg tccatcagga tcacgtcgat gccgacgatc tcctcgcccc ccgggtcggc
     4561 cgaactggcc tcgcgcacct tgcggatcgc ctcggggccg gtgtcggcct ctccggcgac
     4621 cacgatgtcg ccggtgtcgg ccaggatctg cttgatgccc tggcgcacga tcaggtggtc
     4681 gtcgcagatc agcacgcgga tcatggcgcc cctttcacga aggctgtgtt tgccgtgccc
     4741 ggtgggcgat ccgagctggc cgtcagtccg tccccgtcgc gctcgggcat cggcatcgtc
     4801 agccgcacgc aggtgccgtg gttcggaacg ctgtcgatcg cgaggctccc gccgaaatgg
     4861 ccggcgcgct cgcgcatgcc catcacgccg tagctgcgcg ggtgctccag cgccgaaggc
     4921 tcggcgccga tgccgtcatc gcgcacctcg atgtacagct ccgggtgcgg cgggccatcg
     4981 acatagagcc ggattcgaac cgagcgtgcc tgcgcatggc gcgcgacgtt gctcagcatt
     5041 tcctgaaaga tccggaacac cgcgatcgac cagcggtcgc cgtcagggcc actcggcggc
     5101 tcggtctgcg ctgcgacgtg catctgcagg tcggatgcca gttcggtcgt ctcgatgaac
     5161 tcctgcgcct gccactcgag cgcggcccac aggccctggt ggtcgaggat gctcgggcgc
     5221 aggtcggtga tgatgcggcc gaggttgtcc accgcggtgt cgatcgtgcg acccatgcca
     5281 tggcacttgc agctgagttc gggtcggtcc tgaacccgct tctcgaccca gccgacgtcc
     5341 atcttcagcg cgacgagcag cgagcccagt tcgtcgtgga tctcgcgggc gatgcgggtg
     5401 cgctcttcct cgcgcaccga ttccgaatac gcggacagct cgcgcagcgc gccgagcgcc
     5461 tgggtcagct cggccgtgcg ctcgcccacc cggcgttcca gctgctcgtg cgactgctgc
     5521 agcagtgcct cgctgcgctt gcgctcggtg atgtcgacga tggtgaccac cgcgccctgc
     5581 acgacgccgc catcgaggat cgggtgcgac gagtattcgg cagagaacgg gctgccgtcg
     5641 gagcgccaca gcacttcgct gtcgatgcgg cacggcaggc cctgccggaa cgcgttgaag
     5701 atcgggcagt cggcctcggg gtaaggctga ccatccgcgt gggtgtggtg catcagctcg
     5761 tgcatgttgc ggccgagcac cgcgtcgttc gggtagccca gcatctcggc ggcagcgcgg
     5821 ttgacgaagg tgcagatgcc gaacatgtcg acgcagaaga tgccttcacc ggtcgattcg
     5881 agcagcagtc gcaactggtc ggaccacacg ctgcccgttg cgtccgaaga gacgcttgcg
     5941 tcgacggtcg agggtcgtgt cgggagaggg ctcacaagga aggtcatgca caagtccggt
     6001 gcgattcagg agggtacgca aagggcgtgc cagagaccgc acctcgggag tcaggcacgg
     6061 agctggcgct tgacggccct tcggtttcgc gtgccgcgcg gggcgcctgt cgtgccgacg
     6121 ttcccgtcgt cggcttgccc cggcagcgcg gtcagtcggg ctgcaacggc tggcacccga
     6181 caatcgggct ctcaatcgaa agcacgcatg tccaaaccac agacacctcc agccaaacac
     6241 atcagccgtg acctgaccga cgccgagttc tcggaactcg acgacctgct ggccgaaacg
     6301 cccgagccgt tcgagccggt cgacgtggtg atgctcgatg gcttcctatg cggcgtgctg
     6361 gtgcagccgg tgctgctcga cagcgcgacc tggctgccgc acgttttcga cttcgacggg
     6421 cggccgctgc ccgacgatgt cgatccggca tggctcgagc gcatcaccgc gctggtcctc
     6481 aggaggcacg ctgccctgac ccgcgccatc gccgaggacg gctggttcga tccgctggtg
     6541 ctcgaattcg acgaggacca cccggcggag ccgccggccg atggtggccc tgacccgctc
     6601 gctggattgc acccggtgtc gcaatcgctg atgccgtggg ttgccgggtt ccagcacgcc
     6661 acgctgtgct ttcccgacct ttccgagatg accgatgacg cggtgctgtc ggcactggct
     6721 cgcctgtacc gacacctgcc ggcagagacc gacgacgagc gggaggtggt cgccacgctc
     6781 gaccgcgagc acccgctgac cacgctcgaa gatggcatcg aggacatcgt gatcgcggtg
     6841 gccgacctgt ccgacctgac ccgcaaggcg cgcttcaagg tcgagaccgt caggcgcgac
     6901 gttccgaagg ctggccgcaa cgacccgtgc ccctgcggca gcggcaagaa attcaaggcc
     6961 tgtcacggca agacatgacg caccccgggc gaggcgctct tgccgctggg gcgcggaggg
     7021 gcgatcccct cgagaagccg gggcgttgga cgccgtggga cgacccgcct gcggctggcg
     7081 cctgaaattc acgcgagcga gccggaacaa cccatgcgtc tgcaactgtt gtcagacctg
     7141 cacctggaaa ccgaggtctt cgagccacgg cctgcgcctg gcgccgaatt gctggtgctg
     7201 gccggtgaca tcgacagcac ctggcgcgaa ctggaccggt tcggcggctg gccggtgccg
     7261 gtgcttttcg tggcgggcaa ccacgagttc gaccagcgcg agctcgacga tgcgtggccg
     7321 gcgctgcgcg agcgctgcga tgcactcggc gtcaagctga tggagcggca gagctgtgtg
     7381 gtgaccgatt cgaccgggcg ccgcatccgc tttgtcgcga ccacgcgctg gagcgatttc
     7441 gacctcttcg gcgctgccca gcgggatcgt tcgatgcgcg cggccaccta cttcgtcgat
     7501 gtgatgcggt cgaccctgca cggtgcgctg ttcgatgcgg cggcggttcg cgtcgagtcg
     7561 ctggattgcc gcgcctggct ggctgatgaa ctggcgcgcc cgccggagtc ggcgtgcgat
     7621 gcctgggacg cgacggtggt cgtcacccac tacggcccga gcatgcgcag tgccgacaag
     7681 cgctacggca agcaaccggg taccgcaagc ttctgcaacg ccgacgagga cctgatgccc
     7741 cgggccgatc tgtggatcca cggccacctg cactgccgtc atgattaccg cgtcgcccat
     7801 gcggagggcg agacgcgggt ggtgtgcaat gcccgtggcc acagccgcaa gggcgaggcc
     7861 gatcactacg acgggctgtt cacgctcgag gtgtgaacgc cgcaggcgct cttcaggggc
     7921 ggctgttcag gccgtcgaag caggtgacca gcagcagttc gatgatgcgt tcctcggaat
     7981 gctgccccga cagcttcagc aggcccagca ccggatcgca ggcccttgca aagagcgtgt
     8041 acagcacgat ctctgggggc aactggcggt tcaggtgacc tgcctcctgg gctgccacga
     8101 tccaggcgcc gagcttctcg ctgacatcca tcagccgctg caggtaagcc tcgttgctgg
     8161 tcagcgcgga tcgaagtgac gagttctgcg acggcagcga cggcatctcg ccggccagtt
     8221 gcaccttcat ggtccagctc gccacggcct tcaggtggtc gatggcgcgt gcggtcgggt
     8281 ggtgatccag cgtcgcgatg aatgcgagcg cccggtcgag catgcggatc atcgctgctg
     8341 cggccagcgc ttccttcgat ggaaagtgct tgtagaggct ggccttggcg atgccgacgt
     8401 cggcggcgac ctcgtcgacg gtcatgacgt cgtagccttt gtcggccagc aagcggttga
     8461 ctgaggaaac gatcgcgtct tcacgcgctc gcagcatctg ttccttgaaa gatgttcgag
     8521 ccatgcccgc agtgtatggc gggcggtgtc ccgctcagat gacggatcgt ccgttgagcc
     8581 ggtcgacgga ttcaacagca gcgtcagaac tcgcgcgctt cgagtcctgg aatgccttga
     8641 actcttcgtc tgcctgcagg taggcgtcga gcccgaaggc ggttcccatg tcgcccatgt
     8701 cgtcgggatc gcggggcgcg cgcgcagcag cccatcgccc tcccagggcg cgccaccacg
     8761 aaatgcctcg gattcctcgc acgatttgtc tcctcgccaa acgacttgta tttatcgtgc
     8821 cagtctgacg agtccgcgcc gtgacaacgt ggcgaaatga atcggaatga ccgtgtactt
     8881 cgtaacactt cgtccgaatc cgatcattgc ggcagcatcg aaatcagcca ggccgggcca
     8941 tccttgcctt ggcggcgcgg gatcctgcgg caggaggagt agtgatgaaa gtcatcgtga
     9001 tgggcgccgg catcgtcggt gtcagcaccg cgtggtacct gctcgatgag ggccatgagg
     9061 tcaccgtcat cgaccgtcaa ccagacgccg cgctcgaaac cagctttgcc aacggtgggc
     9121 agatttcggt cagcttctgc gagccctggg cgaatgccga cgcgccgttc aaggtcgcca
     9181 aatggctgct gcgcgacgac gcgcccctcc tgttccggcc tcggctcgac ccgcatcagt
     9241 ggcgctgggg cctgagcttc ctcgggcagt gcaacacggc ggccttcgag cgcaacgtga
     9301 gccagctgat cgcgctcggc cgctacagcc aggactcgtt gagaacgctg gtggcgcgga
     9361 ccggcatcga ttaccaccgc ctcgagcgcg gcatcctgca cttcttttcg agcgagcgcg
     9421 acttcgatgc cggggcagcg gctgcgctcg tcatgcgccg tcacggcgtc gatcgccagg
     9481 tgctgtcgcg cgacgaggtg ctgactgtcg agccggcgct ggcagcgttc gccccgcggc
     9541 tcgcaggtgg caccttcacg cccagcgacg aatccggcga tgcgcgcgtt ttcacgcagc
     9601 agctggcgag gcgctgtgcc gagcgtggcg ctgtcttcct gttcgggcat gacatctgcg
     9661 acctgcagaa gtcgaacaac gccatcgagt cggtccgggt gcgcgagcag ctgaccgcgc
     9721 aggtgaagct gttgcgcgcc gatggctatg tggccgccat gggcagccac accgccccgc
     9781 tgctgcgccc gctgggtctg tgcctgaaca tctaccctac caagggctac tcggccatgc
     9841 tgaagctgaa gcagcccgaa cgcgccagcg tggtgagcct gatcgacgac gaccgcaaga
     9901 tcgcgatcag tcgcctgggc gacgaggtcc gcatcgcagg caccgccgaa atggccggct
     9961 acgacacgtc gatcaccagc gcgacctcgc gcgttcgctg tgcagcgctg atccggcgct
    10021 acgaggagct gtttccaggc gtggccgaca cggcccgacc gaactactgg gccggactgc
    10081 gaccgagcac gccggacaac attccgatca tcggccgcag cccgatcggg cggctctgga
    10141 tcaacgccgg acacggcacg ctcggctgga cgcacggcgc aggttccggc cgcgctctcg
    10201 ccgaactgat cagccagaag cagccgacgc tcgatttcgg tttcgctggt ggcgccgccc
    10261 ggccggggcc ggcgccggcg cacgcctgat ggcggactga cctaacggcg ctggggccgc
    10321 cgcgccgggc ctcgcgcggg tggacgattc ggccttgtgg cgtcgggcct cggtcgcgaa
    10381 atgtcggcca gcagcagcgt cgcgcgcacc agctcttcca cggccgcgct caggtcggct
    10441 gccggttcga gcgactcgat ctcctcgatc aggtcgtcgg cgtcctcgag atcgtcggct
    10501 tccaggtggc ggtacagcag cgcgagcggc tcggtcaacg ccttggcttc gagccgcatc
    10561 agcccgggga agaactcctg cgcggtcgcg aagccggcca cccacgggta gacggcgtcg
    10621 gtctcgtgcg gcaggtcgtc tgcatcggac tcgtcctcgt cggattccag ctcgaacacc
    10681 cacggatcga accactgccg ctgcgtgatc gcgtcgttca gctcggcatg tcgtcgcaag
    10741 gcgagcgcat ggagccgctg ggcatcgaac gaggccggca atgcgcgtcc gtccgcatcg
    10801 gtgacgaacg gcaaccactg cgcgggcggg accggttgcg gctgcagcag cacgccgcac
    10861 aggaaaccgt cgagcatgct gacgtccagc ggttcgaggg gcgccggaac gcggtcgagc
    10921 aggacctgca actcgtcgat gtcgtgttcg tcgagcggct gcgaggcggg gcgcggtcga
    10981 tgcgtggagt tcatgtcgaa ttgtcgccgg gtatcggcga actcaggcgg ccggtccgcg
    11041 ggagaactcg gcttgcaggt gctcgaccag cacccgcacg gcgcgcggcg tgatcgggct
    11101 ccacgggcgc atcgcgtaga tcgcgtcgcc gaaatagccg accggtcgcc aggcgggcag
    11161 tacctcgcgc aggcggccgg cgcgcaaggc ggccgccgcg ctgaagtcag gcacctgcgc
    11221 gatgcccagg ccggccagca ccgcatcgcg cagcacctcg ctgttattcg cacacaaggg
    11281 cccctgaacc cccacgctga tgcgctgcgt gtccgacgca ccggccgcga caggcagggc
    11341 ttcgaagcgc cagacggtcg ggcccggtcg caggtagccg aggcaggcgt gcgatgccag
    11401 gtcggacgga tgcagcggcg tgccgcgtcg tcgcaggtag cctgccgagg cgaccagcag
    11461 cgtgcgcgaa tcgcggagct tccaggccac atggttgtcc ggaggcgcga aggtgtggcg
    11521 cacggccaga tcgaacccct cgtgcgcgag gttcagcagg cgatccgaca gttccagctc
    11581 gatgcggacc tcggggtagc gcttgaagaa gccttccagc gaaggggcca cctgctgccg
    11641 ccccagggcg accggggccg tgaggcgaac cagcccgcgc ggctgaccgg ccaggtcgcg
    11701 aacgctgccc aggctctgcg tgatctgctc gaaggctccc gaggtttcgt cggccagttg
    11761 gcggccggcg tcggtgagcc tcactgatcg ggtggtgcgc tgcaccagcg gaacaccgac
    11821 cgcccgttcg agttcgctga gccgctgact caccgccgcc ttgctcagcg ccagccgctg
    11881 tgcggcctgc gtgaagctgc cggtctgcgc cagcacggtc aacaggtgca cgtggggcca
    11941 cagtgcatcg ggtcttgcca tcggaaggtt cggtggttct gggagcgcct attgttcatt
    12001 caaacgaaca atgagatcac ctgaatgatg cgcggctggt cggctgccgc cctagactgc
    12061 cggctccgac accctgatcc cggagcacac gatgggcgca cccgacgcaa tgacccgcac
    12121 ctacgcctcg accgccgagc tggggcattt catcgacggt cacgcggtgg ccagcaccag
    12181 cggacgccgt caggcggtct acaacccggc aaccggcgcg gtggcgcggc aggtcgcact
    12241 ggccagtgcc ggggaggtga acgttgcggt cgccgccgcg caggccgcat tcccggcctg
    12301 ggccgacacg ccgccgatcc ggcgcgctcg cgtgatgttc aagttcctcg aactggtcaa
    12361 cctgcaccgc gatgagctgg ccgcgatgat caccgccgag cacggcaagg tgttcaccga
    12421 cgcgcagggc gaggtcagcc gcggcatcga catcatcgag ttcgcctgcg gcattccgca
    12481 gctcctgaag ggcgattaca ccgagcaggt ctcgaccggc atcgacaact ggacgctgcg
    12541 ccagccgctg ggcgtggtgg ccggcgtcac gccgttcaac tttcccgtga tggtgccttg
    12601 ctggatgttt ccggtggcca ttgcggcggg caacaccttc gtgctgaagc ccagcgagcg
    12661 cgacccgtcg ccgagccttt tcatggccga actgctgcgc gaagcgggtc tgcccgacgg
    12721 cgtgttcaac gtggtgcagg gggacaagct ggcggtcgac acgctgctgt cgcatcccga
    12781 cgtcaaggcc ctcagtttcg tcgggtcgac gccgatcgcg cagtacatct acgagaccgg
    12841 cgcccaccac ggcaagcgcg tgcaggcgct gggtggtgcg aagaaccaca tggtcgtgat
    12901 gcccgatgcc gatctcgagc agacggtcga cgcgctgatc ggcgccggtt acggatcggc
    12961 cggcgaacgc tgcatggcga tcagcgttgc ggtggcggtg ggcgacgtgg ccgaccggct
    13021 ggtgccggcg ctggctgcgc gcgccaggac gttgaagata cgcaatggca tggatctcga
    13081 cgccgagatg ggtccgatcg tcacgcgcca ggcgctggag cgcatcgagg gttacatcgt
    13141 caccggcgtg gccgaaggcg ccacgctggt cgtcgacggg cgcggctaca aggtgccggg
    13201 ccatgagaac ggcttcttca ccggcggcac gctgttcgac cacgtgacgc ccgggatgcg
    13261 catctacaag gaagagatct tcggtccggt gctcgcggtg gtgcgcgttc ccgatttcgc
    13321 cgcggccgtg cagctggtca acgaccacga gttcggcaac ggtgtcgcct gcttcaccag
    13381 cgacggcaat gtcgcgcgcg agttcgcgag gcgcatccag gtcggcatgg tcggcatcaa
    13441 cgtgccgatc ccggtgccga tggcatggca tggcttcggt ggctggaaga agagcctgtt
    13501 cggcgacatg cacgcctatg gcgaggaagg cgtgcgcttc tacaccaagc agaagagcgt
    13561 gatgcagcgc tggccggcca gcatcgcgaa gggcgccgaa ttcgtgatgc cgacggcgaa
    13621 gtagagcccc ccagtcgctg cgctgccgcc ttcagcgacg ctcggtcacc agcggcgaca
    13681 tggccgcggt ggcgcgcacg cgttgcgcca cgcgcagcgc ttcgtcgcgg ctcgcatacg
    13741 gaccggcctg cacgcgcagc agctgctgat cgctgaagac gcccagcagg ggcgccagcc
    13801 acgacaggtc cagcgcgagg cgttgatgca atcggtcggc tctttcgcgc tgcttgaagg
    13861 ctccgagctg cacccagaag ccgctcgccg gtgggggagt ggcgggggcc tgggcggtga
    13921 catcgggcac cgagcccgcc tcgcgtgtca ccggtgatcc tccttccgtc ttcgcttctc
    13981 cagcggcagg cagcggggtt gcgacgatgc cggcctggga ctggaccggg ctcgtcgatg
    14041 cggctgtcgt cgcggtgtcc cgtgacgacg gtgctgccgc gatcgctgcc gtcgaagccg
    14101 gccccggcgc cggggccgtg gccacggacc gggcagcgga cccctgccgc cacgaaccgg
    14161 tgcggatggc atcgtgtgtc agccgctcca cctccaccat ccccacgccg cgcagcaggt
    14221 cgagcttcag tgcggcggtg tagctcaagt cgatgatgcg tgtgctgtgg aacggaccac
    14281 ggtcgttgat gcgcaccacc acctcgcggt tgttcgccgg gtttctcacc cgcgcatagc
    14341 tcgggatcgg cagggtcggg tgcgcggccg tcatcgcgta catgtcgtag cgctcgccac
    14401 tcgcggtcga tcggccatgg aacttgcgcc cgtaccagga tgccaggccg cgttggctga
    14461 tcgccacgtc cgccgccagc ggctcatagc gctggccggc gacttcgtaa ggcttgttcg
    14521 gcccgccggg tcggatcgga tcgatccggg gttcggcgtc gggcacgtcg gccaggttcg
    14581 gcggtggcgc gacgggcggg ccgtcgcgct cggctgccgg tgcgggggcg ggtgatgctg
    14641 cgaccggtgg atcctggctg gtcgcgggcg acgaaccggg tggcgcgctc gcgcagccga
    14701 ccagccacaa cgcgctggtc acgacagcgg cgcgggtcgt ctggatcagc acgccgcgca
    14761 gcatgaccct cggcatctca gtgcccgggt gccatcaccg cgtctgcggc cgcttcgagc
    14821 atggccttca gcgtgggctg caggcgcgcg gcgacttcgg ggcggtactc gaagggcgag
    14881 gcttcgttca tgtacaggct ctggcacagc tcgagctgga tggcgtgcac gtgctgcgcc
    14941 gggtgcccgt aatgccgcgt gatgtgtccg cccttgaagc ggccgttgat gaccgagctg
    15001 cagccgctgc cctgcgcccg tgccgtcacc cgctcgagga tggatgcggc gcagctcgtc
    15061 ccaccggcag tgccgaagtt caggtcgggc agcttgccgt cgaacagacg gggcaggtag
    15121 ctcgcgatcg agtgcgcatc ccacagcagc gcgcagccgt gcagtgcctt caatcgggcc
    15181 agctcgatcg tcagtgcctc gtgatacggg cgccagcagg tgtcgagtct gcgggccgtc
    15241 tccacggcgt cgggtgcgcg gccgggacgg tacagcggct ccccgcgaaa ggtttcggtc
    15301 gggcacagcc cggtggtgac ctgtcccgga tacaggctct ggttgtccgg agggcggttc
    15361 aggtcgatca cgttgcgcga gtgcgtggcc tgcagcaccg atgcgccgag atcctgggcg
    15421 aatgcgtaca gccgatcgag gtgccagtcc gtgtctgcca gctccagtgc gatgtcggtg
    15481 tagtcgccgc gcagggcttc ggggatgtgc gtgccgacat gaggaatcga cagcagcatc
    15541 ggccgcgtgc cgcgcacgag ccggtaaggg gggatcactt cgggggtcgt catgcggtgg
    15601 atgtctgagg gccaacgggg cgtggcgaga tctttggtcg ggtttgccgg catcgtcaag
    15661 gcggatcgga tcgtttcgag ggcgacacct gcagcgcgga agacggcaag gccgggatca
    15721 gccgttcgag gtcggcgcgg gtgtgctgcg tgatcacctg ctgtgtgagc cgcacgatct
    15781 cgtcgtcgag cccctgccac tcgccttcgc ggcacagcag gaagaattcg cgccgtccga
    15841 gtgcggtggc gggcaagggc agcacccgca cgtcgccgag gtgatggcgc gactgcagca
    15901 gacacagtgg cgtcgtgatc gcccagccca gtccggcggc caccaggctc atcagcggat
    15961 cggtcgcatc gaattcgaag cgcctcggtg cctgcacccc gaagtggcgc aggcagcgct
    16021 cgacctgttg tcccatcacg gagcgcaggc tgtagcgaat cagcggccag cgggccgccg
    16081 cggcctgcat gtccggcggg tttccggact cgttctcact tgcgggtggc agcaccagca
    16141 cgaacgcttc cgagaacagc gggcgcaccg agattcggcg gtcggcgacc actgcgtcgg
    16201 tgacgatggc catgtcgatg tcacggcgca gcagctgttc ggccaggacg ggcgtcaggc
    16261 ccgaccacat cagcaggtcg cgggcgcggc ccgacagcgc gcggatcaag gccggcccga
    16321 ccgtggcggc gaacgaatcg acacagccca gcagcagccg cgtgccctcg gtcggggcac
    16381 ccgagcgggc gcggtcggcg accgcccgcg cgtgggccag cagctcggtc gcctcctcgc
    16441 gcagtgcccg gccggcggcg gtgggcttgg gcgggcgcag gtcccggtcg agcagcagcg
    16501 tgccggtgtc gcgctcgagg ttcttcacca gctggctgac cgcgctctgc gtgatgccca
    16561 gccgcagcgc agcctgtgtc atcgactggc aatcgcaaca ggccacgaag gcctcgagcg
    16621 cgtgcaggtc gagcgggcgc gccagcgtca tcggacgggg ccgccggtac aggcggctcg
    16681 cggggcggca tcagaaatgc tggggatggg catgagcttc gttactgtcg tcgaccgatc
    16741 gcggatgggc tcctaagctc agcccctttg gcccgaaccg gaagcgctcc cgcatgcaaa
    16801 cctacgacgt gattgtgatc ggcgctggcg tcatcggggc ctcggtggcc taccacctgg
    16861 cgcgcctcgg tgcgaagcgc gtgctggtgc tcgaccgcac gcagatcggt gccggcacca
    16921 cgtcgcagtc gtccggcatc ctgcgcacgc actattcggt gcgcgagaac gtcgcgatgg
    16981 cgcaggactc gtggaaggtg ttcaccgact tcgcgaacta cctcggtgac gaggatgcgg
    17041 ccagcggcct ggtcaagtgc ggctacctga ttgcctcgcc cgacggtccc aagctcaagc
    17101 cactggccgc agcgctcgag gggcagcgcc agcagggcat cgaggttcgg ctgctcgacc
    17161 gccagcaggc cgaggcgctg ttgccgatcg ccgacctgca cgatgccgcg ctgatcggct
    17221 acgaacccga ggccggcttt gcagatgcct atctggtcgc caccagcttc gcccgcgcgg
    17281 cgcggcgtgg cggcgtcaag atcatcgaag gcgtggaagt gcagcaattg ctggtggagc
    17341 agggtcaggt cgtcggtgtc gccaccgatc agggccgctt tctcgccggc accgtgatca
    17401 gcacccagaa catctgggcc acgcagatcg agcagtggac cggcatcacc acgccggtcg
    17461 tctccgagcg ccacaccgtg ctggcactcg aaggtccgca gccctacacg ttcgctatgc
    17521 cggtcttcaa ggacctgggc tccgaaggca tgctgtactg ccgcagctat ggcggtaccc
    17581 agatgctggt gagcgaaggc accgcgggcg aggtgctcgc cggccccgac aacgagcagg
    17641 gcgatgtgtc gctggacacc atcgcgtcgg tcggcgcgca ggcggccgag cgcttcccga
    17701 gcttcgagac cgccggcctg gcctcctcgt ggaccggcgt ctacgacgtg acgcccgact
    17761 ggaacccggt gctcggaagg ctgcccggga tcggcggact ggtggtcggc tacggctttt
    17821 cgggccatgg cttcaagctg tcccccgccg tgggccgcgt tctggcgcag gaggcgctgg
    17881 gcctcacgcc cgacatctct cttgcgccat acgcgctgga gcggttcgcc gcgggtcggc
    17941 cgctggtggg tcagtacgga gtcggcgcgg tttcctgatg gcgctgcgcg ctttcaggct
    18001 cgacgcgctg ccggaagaac cctggcgcaa cggcggtggg cgcacccgca cgatcgcgac
    18061 acggatgcgc gatgcggacg ccgcaggttc ggcggcgcaa gacgaggggc cgccgtggga
    18121 ctggcgcatc agcgtcgcga cgatcgagcg cggtggcccg ttctcgtcct tcccgggcgt
    18181 cgaccgcagc agcatgctgc tggatgcagg gcggatcgag ctgagtgcag cgggagagcc
    18241 gacgctgctg atgcgaagcc ccggagacgt cgtgaggtac cggggcgaca gcgtcaccac
    18301 ctccgcggtg catggcgcca gtcagcctct gtccgtgctc aacgtgatga cgcgtcggtc
    18361 tgcggcgcag gcctggatga aggcactgcg cgacgattcc tatctggccg ctcaggggct
    18421 ggccctgctg gtcctggacg gcagctggcg cgtggtggac gggtcgatgg gtgccgaaga
    18481 cccggatcac ggcctgaccc tgcactccgg cgaaggtgcc tggtgcacag tgtctgcacc
    18541 cggttccagg gccatctggc gcgtcgagcg gctgtcaccg acagggatgc tggccgccat
    18601 tgagctggat cgcagttgac ctgttccgtc tcggctgatc tggcctgagt ggcaatcact
    18661 cggtgattcc gatgtcggtg cgtcacggat cttcctgtag agaaggggag gttcgctgcc
    18721 gccgccgtgg gaaacgccgc cggcgtgacg ctcaatccgg atgggacagc accgtggcca
    18781 tcgcgaccgc tgtggcctcg acgttggccg agttcaaacc ggccacgcaa atgcgacccg
    18841 agcgcagcac atagacggca tgctcctcgc gcagccggtc ggcctgctgc gggctcaggc
    18901 cggtgtagct gaacatgccg cgctgcgtga tgaagtagtc gaagtcgcgg cccggcagct
    18961 tggcgctcag caccgcgtgc aggcggtggc gcatcgcgcg gatgcggtcg cgcatcgcgt
    19021 cgagctcggc ggcccacttt gcgcgcagcg ccggggtctg gagcacacgg gcgacgatct
    19081 ggccgccgtg ggtcggcggg ctgctgtagt tcttgcgcac cgtcgcgcgc agctggccga
    19141 gcaccagttc ggcttcggcg gcattcgggc acaccacgct cagtccgccg caacgctcgc
    19201 catacagcga gaagctcttc gagaacgagc tggcgcagaa gaatgcgatg cctgcatcgg
    19261 ccagcgcgcg gatcgcgaac gcgtcttcat cgatgccgtc accgaagccc tggtaggcga
    19321 tgtcgacgta ggggatcagc ttgcgcgcgc ggatcaccgg gatcagttca gcccactgcg
    19381 ctgccttcag atcgacgccg gtcgggttgt ggcagcaggc gtgcatcagc acgatgctgt
    19441 gttcgggcag cgccttgaac gcggcgagca tcgcgtcgaa cctgaggccc ccggtggcgc
    19501 cgtcgtagta cgggtaggtg ttcaccgtga acccggcgcc ctcgaacatc gcgcggtggt
    19561 tgtcccaggt cgggtcgctg acccagacct gcgagtccgg gaagtagcgc ttcaggaaat
    19621 cgccgccgac cttcaggccg cccgatccgc cgatggtctg caccgtggcg atgcgtccgc
    19681 tggtcaccgc ttcatgggtt gccccgaaca gcaggtgctg caccgcctga cggtagttca
    19741 cggcgccctc catgggctgg taggggcgcg cgccgatggt ctgcagcatc gcactctcag
    19801 cctcgcgaac cgcgcccatc actggcagct tgcccgcatc gtcgaagtag atgccgatcg
    19861 acaggttgat cttgtgcggg cgcgggtcgc ggccgaaggt ctcgttcagc gtgaggatcg
    19921 ggtcgccggc gtaggggtcg atgtgttcga acatgaaagg gtcgatggga tcgtgtggga
    19981 gaaatgcagg cgacgggtca ggccgacaag gcggcctcga tgtcggcctt gagggaggcg
    20041 ggtgtgtccg tgggggcgta gcgcttgagc acctgcccgt cgcgaccgac caggaacttg
    20101 gtgaagttcc acttgacggc cttggtgccg agcaggcccg gcgcctcggc gcacagccat
    20161 gtccagagcg ggtgcgcgct gctgccgttg acttcgatct tgctcatcat cgggaagctg
    20221 acgccgtagt tcttctggca gaaggcaccg atctccggat tgctgcccgg gtcctgcgag
    20281 ccgaactggt tgcatgggaa accgatcacc gtcagtccgc gttcgccgta ggtcttccac
    20341 agatcctcca gaccggcgaa ttgcggcgtg aagccgcagg cgctcgccgt gttgacgatc
    20401 agcagcacgc agccacgctg ggccgagagc gggacggtcg tgccatcaag ggcggttgcc
    20461 tcgaagtcga aggcgctgcg agcagatgcg ttcatgggac ctcctgggtg ggcggcggtg
    20521 gtcgtgtcca gcccgggatc gtagcgttcg aggcaggtgc cgtcgccgta ccctccccgg
    20581 cgggcaggct tttcagtgcg ctccgtcgcg tgttgcccag acggcggccg ccacgatcat
    20641 cgcgccgccc agcaacagcg ggacggtgat gcgtcccgcc cccagcagcg cggccgaacc
    20701 ggcggcgacc aggacctcgc tgagcatcag aaccgaggtc acatggatcg gcagccgcga
    20761 ggcgccgaac tgcagtgcca gattgctgca caggaacagc acgctcatcg tcagcgccag
    20821 ggcgatcgcc caccacggca gtgcagcaac gccgcctgcc aggggccacg gaacgcggcc
    20881 cagcgcactg agggcggccg cgagcgcgcc tgcgacgacg gcgccgccga cgaacatggc
    20941 cagtgcacga ccttcctcgg gccgatctgc ctcgcgcttg agcatcacgt tgttcagcgc
    21001 gaaggtgaat cctccgagca ggcccagccc gtcggccagc gattgcggaa acggcagcac
    21061 ggcccagccc gtcgcgccct cggggcgcag cacgatgccc gcgccgagca cggcgagtgc
    21121 gatgcgggcg ccggcggcca gcgtcaccgg ctccttgagc agtacccgcg ccagcagaac
    21181 cgaccacaac ggcatcaggt agaaaagcag caccacgcgc accacgtcgc cgatcacggt
    21241 gccccagttg aaggcggcgt tcgtcagtcc ggcggccagg gccagcaccc acagcggcca
    21301 ctgggtcagc agctgaccga ctgcgcgcgg ccgccaggct gcgatcacca gcacggtcgg
    21361 ggcgtagatc agcaccgtcg cccacagcgg gtgcacgccc tgcgcgtcga gctgacgcag
    21421 cggccaccac gacaggcccc acaccagggc attgaatacc agtgcgagcg cggcccgacc
    21481 ggcgagctgg ctggccactc ccggctcagt gctggtcgct gcgcgccagc gcgagcaggt
    21541 gatcgacctc gacgcgatga tggatgcagt tgcgctgatg ccagcggcgg atgagccaca
    21601 tcaggccggc gacgatgacg ccgaagatcg tgatcgcacc gaatgccgac aggcccagct
    21661 tcgtcatgcc ggcgtacaac gcgccgagtc cgaggatgca ggcctgttcg ttgaagttct
    21721 gcaccgcgat cgagcggccg gctcccatca ggttgtgacc gcggtgctgc agcagcgcgt
    21781 tcatcggcac caccaggaag ccgccgagcg caccgagcaa caccaggaac ggcgctgcga
    21841 tccacacatt ggtgatgaag ttcatgccga tcaccagcag ccccatcgtg atgcccagcg
    21901 ggatcacggc agtggcctgg tcgagtcgca tgcgaagaga tgccgcgacc gcgccggcgg
    21961 cggtgccgat cgccaccacg ccgaccaggg acgacgccag cgtggtgtcg tagcccaggg
    22021 ccacgctggc ccaggccagc acgatgtagc gcaggttgcc cgacacgccc cagaacagcg
    22081 tcgtcgtgga aagcgagatc tgccccagct tgtcctgcca caggcgcgca ttgcagcggg
    22141 cgaagtcgcg caccagcccg atcaggctgt gggcgatcgg ctgcagcggg gcctcggtgc
    22201 gcgggatgcg cagattgaac gccgcagcca gcgcatacag cagcaccatc gacgcaatgg
    22261 ccgcttcggc cggcgagtcg atgccggtgt cgatcaaggg gaagtcgaaa gccagcagga
    22321 attccgagat ccggtgtccg acgagctgac cgccgagcag cacgcccagg atgatcgaca
    22381 ggatggtcag gccttcgatc cagccgttcg ccttgaccag ctgggagttg ggcagcaact
    22441 ccgtcaggat gccgtacttg gccggggaat acgcagccgc tcccagcccg acgatcgcgt
    22501 acgccatcag cggatggctg ccgaacagca tcatcaggca gcccaccacc ttgatgaggt
    22561 tcgagaagaa cattaccctg cccttgggca cggcgtcggc gatggcaccg acgaacggcg
    22621 caagcaacac atagaacagc gcgaacatcg gcaccagtgc ggcgcgctgc cactcgggct
    22681 ggccggagct cttcaacagt tctaccgccg ccacgaacag cgcgttatca gccagcgagc
    22741 tgaagaactg cgccgccatg atggtgtaga aacctttttt cattcgctcg tggcgcgcgg
    22801 cccctcggat cggcatgacg aagccgtaca cccgacggga ctccttgcgg cgcgggttat
    22861 agcacgcggg gtatggccga ccggggggct caggccccgg ctggcagggg catgcggcgc
    22921 gtgcggcaga atggttttca tgccccgccc catccgcgcc gtgatccaca ccgaagccct
    22981 ggcgcacaac ctgcgccgca ttcgtgcaca ggcgcccgat gcccgggtct gggcggtggt
    23041 caaggcaaat gcctatggcc acggcatcga gcgggcattc cccggtctgc tgggggccga
    23101 tggcttcgca ctgcttgatc tcgatgaggc gcagcgcgtc cggtcgctcg gctggcgcgg
    23161 gccggtgctg ctgctagaag gatgcttcga ggcgcgcgac ctcgagctgt gttcgcgcct
    23221 gaacctgtgg cacacggtgc atcgcgaggc acagatcgac tggctggctg ttcacaagac
    23281 gcagcagccg caccaggtgt tcctgaagat gaacagcggg atgaaccgtc tgggcttcac
    23341 gccggcggcg taccgctcgg cctggctgcg cctgagcggg ctgacgcagg tcgagagcat
    23401 cacgctgatg acccactttg ccgatgccga acaggcgggc gatctgcacg gacgccgtgc
    23461 catcgatcgg cagctggcgg tgttcgaggc cgcgacgcaa gggctgtcgg gggatcgaac
    23521 gctctgcaac agcgccgcga ccctgcgcca tgcgcccgac gacgcggcgg ttcgtgccga
    23581 ctggatccgc cccggcatcg cgctgtacgg gtcggcgccg gacttcccgc aatgcagcat
    23641 ctccgactgg aaactgcgtc cgaccatgac cctgcgcagc gaactgattg cgacgcagga
    23701 tctgcagccc ggcgacacgg tgggctacgg cagctgctac aacgccgagc gcccgatgcg
    23761 aatcggcgtc gtcgcctgtg gctacgccga tggctacccg cgcagtgcgc ccaccggcac
    23821 gccggtgctg gtcgatggcg tgcgtgcccc gctgatcggt cgagtgtcga tggacatggt
    23881 caccgtcgac ctgggtccgg ttcctgcggc acgcatcggc agcgaggtca cgctgtgggg
    23941 ctgcggtccg ggagattcgg tgctgccgat cgacgaggtc gcgaacgccg ccggtaccgt
    24001 gggctacgag ttgatgtgcg cgctggcgcc gcgggttccg acgcatccgg gatgaagggc
    24061 tcgcatcgca gcgcggcggg cttcgagcgt cgggtccgtg ccgatctgaa ggctcgacgt
    24121 tggctgcgct ggcatgcatt cctgctgggc ctgctgtgct tcgcgacgct gtggtccgtg
    24181 tccgccggac tgatgcacgc aggaatcgag acgctggcgc tgcgctggcc gctggcattt
    24241 gcctttgcct acgctgcatt catcgggctg ctgtggctgt ggtgccgttg gctgctgtcg
    24301 cacgatgaat tcgatgccga cctcaacatc gactggaacc tcgacctgcc ccgtctcgat
    24361 ctcggccgcg gtggcgcgcc gttcagagcg ggtggcggcg gcgatttcgg cggaggcggt
    24421 gcgagcgcct cgttcgacgg gttgcctggg gccgatgccg ccagcgaagt ggcccagacc
    24481 gccctcgacg ccgtcgcgtc gtccgacgag ggcgttgtcg tggcggtccc gctggcggtc
    24541 gtggtgggaa tcgcgctgct gatcgcaggg gccatcggat tcgcggtgtt cgggctgttc
    24601 ggcatcgacg tgctcctcgg tgttgccgtc gagatcgcat ttgcgtctgc cggcggggcg
    24661 ctggcctttc gcgcgcagcg cgaggggtgg ctggcgcacg ccttgtcgcg cacggtgtgg
    24721 cccgcggtcg cgttgctgct gatcttcgcg gttctcggcc tggtcatcga ccgctggctg
    24781 ccgcaggccc actcgctgcc gcaggccctg cgcctgctgc acggttgaac tgcgcatcgt
    24841 gattcgccac ttcgaagttc ctcccggcga ggtcgagatc accgcgatcc gcgcccaggg
    24901 tgccggcggg cagaacgtca acaaggtgtc gagcgcggtg cacctgcggt tcgacgttca
    24961 tgcatcgtcg ctgcccgacg acgtgaagca gcgcctgctc tcgctgcgcg accaacgcat
    25021 caccgccgaa ggcgtggtcg ttatcaaggc gcagacctcg cgcagccagg acgccaaccg
    25081 cgccgatgcg ctggcgcgac tgcaggaact ggtcgacagc gtcaccacgc cgccgcgtgt
    25141 gcgccgggcg acgaagccca cctatggctc caagcagcgg cggctcgagg gcaagagcat
    25201 ccgcgcgcag gtcaaggcgt cgcgcggcaa gatcggacac gagtgatctc cggtcggctc
    25261 gcggctacag tcgtcgggtg agcaaaccca agtctctcta cacctgttcc gaatgcggag
    25321 gcaccagccc gaagtggctc ggcaggtgcc cttcgtgcga ggcctggaac acgctggtcg
    25381 agtcggtcgc cgaaagcacg gggccgaagc accgctacca gtcgctggcc ggggcgttgc
    25441 cggtggcgac cctggccgac atcgaggcct cggatgtcga tcgtctgccc acgggcctcg
    25501 atgagctcga ccgcgtgctg ggtggcggca tcgtggccgg tggcgtggtg ctgatcggag
    25561 gcgacccggg catcggcaag tcgaccctgc tgctgcaggc actcgatgcg ctgtcccgaa
    25621 atgtgcgcac gctgtacgtg accggtgagg agtcgggcgc gcaggtcgca ttgcgctcgc
    25681 ggcggctggg gctgggcgag gcgaccggag gttcgcaggt ccgcgtggtc gccgagatcc
    25741 agctggagaa gatcgcggcc accatcgctg ccgagcagcc ggtggtctgc gtgatcgatt
    25801 cgatccagac ggtctacagc gaccagttga cttccgcacc cggctcggtc gcgcaggtgc
    25861 gcgaatgtgc ggcgcagctc acgcgcattg cgaagtcgag cggcacgacg atcgtgctgg
    25921 tcggccacgt gaccaaggaa ggcgcgctgg cggggccgcg cgtgctcgag cacatcgtcg
    25981 acaccgtgct gtacttcgag ggcgacacgc acagcagcta ccggctggtg cgcgcgatca
    26041 agaaccgctt cggcgcggtc aacgagatcg gcgtcttcgc gatgaccgag cgcggactca
    26101 agggggtggc caacccgagc gcgatcttcc tgtcgacgca cggcgatccc gtcgcggggt
    26161 cgtgcgtgct ggtcacgctc gaaggaacgc gtccgctgct ggtcgagatc caggcactgg
    26221 tcgacagcgg gggacccagc ccgcggcgac tgagcgtggg tctcgatcgc gaccgcctgg
    26281 ccatgatgct ggcggttctg caccgtcacg ccggcatcgc ctgctacgac caggacgtct
    26341 tcgtcaacgc ggtcggcggc gtgcgcatcg ccgagccggc cgccgacctg gcggtgatgc
    26401 tggcgatcca gagcagcctg cgcggcaagc cgctgccgcg gggcttcttc gccttcggcg
    26461 aagtcgggct ggccggcgag gtgcgccccg caccccgcgg tcaggaacgc ctgaaggagg
    26521 ccgccaagct cggtttcagc gtcgccgtgg tgcccaaggc gaatgcgccc aggaagccga
    26581 tcgagggcct gaccatccac gcggtcgagc gcatcgagga agcgatcgat cgcgtgcggg
    26641 cgttgtgacg cagccgcgtc gactgcgaaa gcagccgggc cctaaaatct agggttgact
    26701 gacagtttgg agacacgcca tgtcgatggc cgatcgcgac ggaaagatct ggatggatgg
    26761 cgaactcgtg gagtggcgcg acgccaagat ccacgtgttg tctcacacgt tgcactacgg
    26821 atgcggcgcc ttcgagggcg tgcgggccta caagacgcag caggggaccg ccatcttccg
    26881 gctgcgcgag cacaccgaac ggctcttcaa cagcgccaag atcctccgga tgaagatccc
    26941 gttttccatc gagcaggtga tggaggcaca gcgccgcgtc gtgctcgaga acaagctgga
    27001 aagcggctac atccgtccgc tgacctggat tggcgacaag aagctcggcg tcagcccgaa
    27061 gggcaacacg atccacctga tggtggccgc ctggacctgg ggcgcgtacc tgggcgagga
    27121 cggcatgaag cgcggcatcc gcgtcaagac cagcagctac acgcgccacc acgtcaacat
    27181 cacga
//
LOCUS       DAOORV010000037        26905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2138_length_26905_cov_10.267039, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000037 DAOORV010000000
VERSION     DAOORV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_26905_cov_10.267039"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PKV56_07035"
     CDS             complement(<1..1519)
                     /locus_tag="PKV56_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67605.1"
                     /translation="MTNLPPIHITLMQPAGYVHSLGLLDQARYVRYQLQRLGATVTIA
                     KNRVREDALNIVFGAHLGFPPDWRRRHACVFFNLEQLGSGGAPVSDDYIKLLRGSAVA
                     DYDAANIPAYAADAAEVPVLPFLHAPYLESAGTMPIAERPIDLLFFGSMNERRRAFIN
                     RIEACGVSVSMFDHPLYGEERDAYIRQSKAVLNCSFYETSRFEQARVFQCLSMGTPVI
                     SEHHADAHVPAAFTDSVFWIDPAGHALETFFTKHFGTGAYASEAGQKLASFRGHDPIE
                     SYADLLWFACGYLEGHGITRLGTGDTGAWRPSHINLGSGKDYKPGWLNLDVQDRAEPD
                     AVLDIGQPITLPLVIENRYGGQIELAAGTIDRLYANNVLEHVPDLSLLMTNALALLKD
                     GGRFDIEVPYERALTAWQDPTHVRALNQNSWIYYTEWFWYLGWFEHRFNMIDSTYLDL
                     KLQPCTQEHAAFMRVAFMKVETTARERNIARTMQADLRLPDDEFAPAPERRPVVPV"
     gene            1963..3123
                     /locus_tag="PKV56_07040"
     CDS             1963..3123
                     /locus_tag="PKV56_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOX67606.1"
                     /translation="MSKDSSKDRDSSDDNSTVSGSSSNDGYQFTIVGGAVTAVFEIED
                     GATRAKQMKSNETWTVVGDQVVKTEVEHGYTEVYTFADANGDGIYLKISEVHTDSTTG
                     LPISNGDFGDDSSSSWSGSGGSESHHGRGGDDVLEGNAGDDRLYGEDGDDHLHGGDDD
                     DYLSGGSGINTVVGGAGDDLIDATGGTGKDLYDGSEGVDTVNYGAATKGLKVDLNSGS
                     AKGSEFGKDKLISIENVVTGSGNDKVSGSTADNRIESGAGNDQISAGDGNDWLSGGLG
                     FDKLTGGKGADTFDFNSLDELGLGKATDSIKDFRHAEGDRIDLSGIDANASVAGDQAF
                     TFLSAAPVDGDQTGAVWFSKKALYISTDADAQAEFQINLSGVSALVVEDLIL"
     gene            3269..4972
                     /locus_tag="PKV56_07045"
     CDS             3269..4972
                     /locus_tag="PKV56_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOX67607.1"
                     /translation="MNPPVTPSELRSAAAELRPQLVRATAFSILAGLLVLAPTGYMFA
                     VYDRVLNSRSPTTLAMLTLLVLGAYVVMEVLEWARGELLREAALALDRRLSNRVFEAI
                     FEADRSNLPGGTTQPMSDLRTLREFLNAPVLPALMELPVSLVFLLLLFAISPLLGGSA
                     LVGALLQTGLTWLSERATQPPLLAATRGAAAAQQYADAAMRDAEAIESMGMLDAIHRR
                     WSVKQREFLRLQAQASDAAGGYQAASRFVQQVLGSLLLGLGAWLLLRDEIPGGAGMLI
                     VGSILGARVLAPMVQVVSNWRQVVTVRDAWARLDALLAQWPARTASMPLPTPRGVLSV
                     DGLHAGVPGSPANILRGLQFALSPGEALAVIGPSASGKTTLARLLLGLWPAASGKVRL
                     DGVDVHGWDKAELGPHVGYLPQGAGLFEGTLAENIARFGEIDMAQVEAAARGVGLHDF
                     IVALDRGYDTPVGADGARLSGGQRQRVALARALYGEPVFVVLDEPNSSLDEAGEAALA
                     AAIAAGKRRGATFVVITHRKSVLDVVDKMLVLRDGVQQLFGPRDEVMARFGSAPQAAR
                     A"
     gene            4989..6734
                     /locus_tag="PKV56_07050"
     CDS             4989..6734
                     /locus_tag="PKV56_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOX67608.1"
                     /translation="MTTSTERVPRRELPQTLWSLRRAFALVAVFSLVVNVLMLAPTLY
                     MLQVYDRVLASRSELTLLAVSLITLFLLAVMATSEAVRSRVLVRAGRRLDERLSSRVF
                     HASFAAGLGPSAGVPSRAFGDLIELRQFLTGPGIFALCDAPWSPVYIAVLFLLHPWLG
                     VMAIGFVLNQAALAWFGHRHTVAPLAVAGEAQAEATAYLKSKLRHAEVIEAMGMLGQL
                     RQRWARRHQNHLARQHAALGLAGRVTACSRFVRYAQQSLALGAGALLVIDGQLSPGAM
                     IAANVLMTRALVPIDQLVSAWRGFVGARDAFARLGRLLDAHPERPRIVATAIPSGAVR
                     LSDVVARAPGRAAPLLDGVSLSVAAGTVTVVLGPSGSGKSTLARVLVGLWPCDSGSML
                     LDGLPIDHWSRCELGPHIGYLPQDVELFEGSIAENIARFGEVDAARVIEAARAAGLHE
                     TILRFPKGYDTPVGAEGGLLSGGQRQRIGLARALHGDPALVVLDEPNANLDDAGEAAL
                     LAAVQGCRERGRTVFLISHRPGIVEAADRLLILREGRVQIEGPREAVVAALKSAQPPA
                     IHPAPLRPEPVSSPS"
     gene            6731..8083
                     /locus_tag="PKV56_07055"
     CDS             6731..8083
                     /locus_tag="PKV56_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOX67609.1"
                     /translation="MNVDASSSPSRMAGTDPQDPPLSSGTRRAARIGWWALGAGLGAF
                     LIWAALAPLDEGVPMPGQVSLDTKRKPVQHLAGGIVRQVMVHEGAHVDAGQALMTLVE
                     TTARANHEAVRQRYLGLRAVQGRLLAEQAGQTAIELHPDLIAAADDPSAQAQISTQHQ
                     LLASRRLSLRADLQAIEENIRGQEALLQSHAAMSAQRRSQQVSLTEQLDAMRGLVTEG
                     YVPRNRQRELERQVADAEAALAEMTGNSARVRRTMAELAQRAVVRQQAYREEVDTQLA
                     DVTREVQGDAQRLVALQAELDRMAIRAPATGQVVGLVVQTVGAVVQPGQTLMDIVPDG
                     QPLLLEAHVAPHLIDKVRVGLPADIRFQSFSQAQQPVVEGRVESVSGDLLTDPQNPQR
                     TYYLARLSVTPAGLKALGERRLQPGMPADIVVRTGERTLLTYLLNPLTRRVAASMKEE
                     "
     gene            8087..9457
                     /locus_tag="PKV56_07060"
     CDS             8087..9457
                     /locus_tag="PKV56_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOX67610.1"
                     /translation="MAPVTDTRAACRRSFARSATLAAALFISLPSPAMDLREAYLAAL
                     DRDATLRVSRAAARVGQERLPQARAQMLPNLSASIGHTRNDLDFSSPDPVTGRSRQND
                     SRYNSNNQTVTLRQPLIRPFQWAQLRQAEAQVADANAKLDNELQNVGVRVAEAYFQTL
                     QADDELALVLAQKTSFEAELDAARKSLAAGVATRVGIEDAQARLDMVAAQELEARQQR
                     DHARLQLQTLVDRPVDQLAPLDLDSLQLLPPEPARVDDWVERALRGNAEMRALQAQLE
                     AARQEVDKARAGHYPTLDATAQWSRSQSENVLNTASRYTNVAVGVQLNVPIFAGGQVD
                     SSVRQALAEQDRVEASLDAMRRDLGLRVHQAYRSVTESILRARALEQSVRAADHLVRS
                     AQKSFEGGSQTRLDVLDAEQRRTGALRDLARARYVCLVSGIRLQALVGGERDAAIDAV
                     NASLKR"
     gene            complement(9462..11375)
                     /locus_tag="PKV56_07065"
     CDS             complement(9462..11375)
                     /locus_tag="PKV56_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67611.1"
                     /translation="MQSERAFLTAKRNAYQHVTKHDWVGAAQDLGALSTDARFSAKDW
                     LSLAIASIHAGSLVDAARAARRALAEDPDNFRVVQVLTIALMEQHRWSEVVEVFESHG
                     HLEAAQESFSFLVNQGTALWQLRRLKESLDVFLRALSLNISDPSVHLRIGLVLKDLKM
                     YDEAAESFLTTVALQPDRLSARLMAIHMRQYACDWRDFDASCSEAVAAMARLNPEQPE
                     HGEGATFALLGIPHPPALFKVAAQQAAAFHTGRVLPLPPQTRERDSAGRIRIGYLSCD
                     FHNHATAQLLVETLEKRNTRDFEVTLYSYGHDDHTAMQHRVRAACEHFVDMRAMGPRE
                     MAERIRADGIDVLVELKGHTQGSRLAVLAYRAAPVQVSWLGFPGTTGMHCIDYFIGDP
                     VATPLSHAAHYTECLAQLPHSYQPNDSQRPRPAPSQRMQWGLPKQALVLGCFNQSFKL
                     TPQTFDAWMRILRAVPNAVLWLLNDNPLATANLRREAQQRGVDPERLIFAAKVSVGEH
                     LARLPLADLMLDNWPCNAHTTASDALWMGVPLATVIGDSFASRVAASLLHAVALDSLV
                     YENVAQYEAGVIHLLRHPEKLRALREHLDRGRSVFPLFDGSRCAQDVEALYRRMVDRS
                     RQGLPPQALAAAS"
     gene            11820..13016
                     /locus_tag="PKV56_07070"
     CDS             11820..13016
                     /locus_tag="PKV56_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOX67612.1"
                     /translation="MAMTINTNVASLTAQRNLSSSQSALSTSMQRLSSGLRVNSAKDD
                     AAGLAIAERMNATTRGLNVAARNSNDGISLAQTAEGALGKVGDMLQRMRELAVQSANA
                     TNSADDRTALQSEVGQLRDEINRVAKQTSFNGTKLLDGSFAAATFQVGAGAGENITIA
                     ALTNASSDQLSNVTYGSGDVTGISTGTVTTLTEVAAGTLAMTVDGTEYELSALGAANS
                     AEERLGQVAEAINRRSSDTGVTAYLSKDASTGDYDLKFRSGNLDSSDAAVAVSFGGFT
                     VAETGIGDTSATIASTAVTGFDDLSVETTEDSWLAIKQIDDALDQVNGARATLGAVQS
                     RFENAVSNIMIQSENTAAARGRIVDADFASETANLSKSQILQQAGTAMVAQANQLPQQ
                     VLSLLR"
     gene            13286..14482
                     /locus_tag="PKV56_07075"
     CDS             13286..14482
                     /locus_tag="PKV56_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOX67613.1"
                     /translation="MPQTINTNINSLTAQRNLNASQGSLSTSMQRLSSGLRVNSAKDD
                     AAGLAIAERMNTQVRGLNVAARNANDGISLAQVAEGALGKVGDMLQRMRELAVQSANA
                     TNSADDRKALQSEVGQLRDEINRVAKQTSFNGTKLLDGSFSAATFQIGANSGEAITIA
                     ALTNASTDGLSNVDYGAGNVTDVSTGTITTLTAVAAGTLAMTVDGTEYDLAALGDARS
                     AEERLGQVAEAINRRASDTGVTAYLSKETDGDYTIEFRSSKLDSTGVPAAVSFGGFTV
                     AETGIGDTSATIAATNATGIADLSVETTEESWLAIKQIDDSLDQINSARATLGAVQSR
                     FENAVSNIMIQSENTAAARGRIMDADFAAETANLSRAQILQQAGTAMVAQANQLPQQV
                     LQLLQG"
     gene            14650..16101
                     /gene="fliD"
                     /locus_tag="PKV56_07080"
     CDS             14650..16101
                     /gene="fliD"
                     /locus_tag="PKV56_07080"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOX67614.1"
                     /translation="MATISSPGLGSGLNVTSIVDQLVAVERRPIDLLTTQKTALQSKL
                     SAFGLLKSYTTNVEDAVARLADPTLWQKTSTGSTDPSSVTATSTAAAKDGSYSVQVGQ
                     LAQAQSLASGAYASNSAAVGTGTLKIELGTWGAGPAFADKVPASSVDIVIGAGDNTLE
                     GVRAKINAANAGVSASIIKDGSGSRLVIRSLATGAENAMRITATGAAALPVLPSDPPS
                     LASLVYNPAVPASSKLTQAVEAKDAQATINGLAVTSKSNTFADVLEGVTLTVSKVTST
                     AAQVSVGIDKDSMKKAVADFVKAYNDISKYLAQQTKYDPDTKTAGDLQGDRATLSLQS
                     QLRNGLMQSSGASTVFRSMSDMGIELQRDGTLTVNDSKLGTALSNPTEVSKAFTSAAT
                     NGPGYTGLAVRLKSLTDSMTLTDGLVTTRSDGLRTSIKRVDDQVAKYEERIAAKRESL
                     LRQYSALDTKLSSLTSLNSYISQQITNWNKSTN"
     gene            16188..16643
                     /gene="fliS"
                     /locus_tag="PKV56_07085"
     CDS             16188..16643
                     /gene="fliS"
                     /locus_tag="PKV56_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOX67615.1"
                     /translation="MPAMFAAANPFRTPSFNAQSNAYRQIGVTSAVDGANPHRLVGML
                     YEGLLESIAEARGALRTGNVEMKCRSVSRAVRIVEEGLKGGLDLQSGGEIAANLSLVY
                     GYVSQRLTMANLQNDDAMLQQCAELIQPLREAWTQIGAAVATQPLVVNG"
     gene            16679..16978
                     /locus_tag="PKV56_07090"
     CDS             16679..16978
                     /locus_tag="PKV56_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HOX67616.1"
                     /translation="MTQNLLQYYEAIERASQDMLEAARNGNWDAVVKLEGACALLISQ
                     LKNAAADRKLAADEAQLKSRIMQRILVNDAEVRVLAEPWLEGLDDLLAGRSATLH"
     gene            16993..17793
                     /locus_tag="PKV56_07095"
     CDS             16993..17793
                     /locus_tag="PKV56_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOX67617.1"
                     /translation="MDTLPMPMDTMPMPMDTLPMPLDAVAAAHGGLDDFRITAPSELA
                     TMLKRLLDAGVVINLNAPNGTAVASFLWTVDAARGAITFSADAHAPQLQALIECNEAV
                     AVAYLDSIKVQFDVHGLMLVHNGRSSAISCGYPREIFRFQRRNGYRVRPLLRNAPTAR
                     LRHPMIPDMQLNLRVLDVSIGGCALFLPEDVPMLEPGVLLNGVDLDLDADTHIVAPLR
                     LQHVTSLHLEARGVRLGCELVNASNESLRALQRYIDQTQKRRRLMVLD"
     gene            17948..18202
                     /locus_tag="PKV56_07100"
     CDS             17948..18202
                     /locus_tag="PKV56_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67618.1"
                     /translation="MSVSSIPAVSRVEPAASSARPSDTPQRPQSEQSSPAVGHKFLEE
                     PEPRRFPWLSWETRELEVASKQPSPYGNIPLLGKELDRQV"
     gene            complement(18230..18553)
                     /gene="fliE"
                     /locus_tag="PKV56_07105"
     CDS             complement(18230..18553)
                     /gene="fliE"
                     /locus_tag="PKV56_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOX67619.1"
                     /translation="MNVTLKPFDFAQAVARAGLNTQGQPLKKVQPTEDGGFQAALTQA
                     IQSVSNSQTQASRMQREVQLDNPNVSIEETMVAMQKAQIGFQATLQVRNKLVQAYSDI
                     MNMQV"
     gene            18770..20446
                     /gene="fliF"
                     /locus_tag="PKV56_07110"
     CDS             18770..20446
                     /gene="fliF"
                     /locus_tag="PKV56_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOX67620.1"
                     /translation="MDNAVTLNSPGAVATAPGFGSRLAALPAKSKFNLGMGAAALVGI
                     VVALLLWSNQGDYKVLYANLSDKDGGAIIAQLSQMNIPYRHSDGGAAILVPASKVHDA
                     RLKLASAGLPKASVGGFEMMDSARFGQTQFQERLTFQRGLEGELSRSIGSLAAVESAR
                     VHLALPNQNGFFREQQKPSASVVLSLHAGRTLDRGQVAGIVHLVSSSVPEMSPKAVSV
                     LDQTGALISSPPDVGQQAGLDAQQLQYVAQVEQGYSKRIFELLEPVVGRENLRANVTA
                     DIDFAQIESTEEAFKPNQGASAPATVRSQQTTEQSGSNPAQAAGVPGATSNQPPLPAA
                     APVTGAAAPLQTAQNGGAVGNSRREAVTNFEVDKTVRVTRNATGNIKRLNAAVVVNNR
                     SVTDPKGKVTQVPLAPEELEKLTALVQESIGFNKERGDSVRVINAPFKVEPITVVDTP
                     IWKQPEIIDMLRAAAVPAGLSLVALLVFFGMIKPGLNAALAPAVPLPGSAIDASVDDV
                     EVLPGLPQGEVRALEAPRNTESLDAARALAKENPAAVAQIVRGWVSGQPA"
     gene            20490..21497
                     /gene="fliG"
                     /locus_tag="PKV56_07115"
     CDS             20490..21497
                     /gene="fliG"
                     /locus_tag="PKV56_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOX67621.1"
                     /translation="MSAESNEQGLEDAAILLMSLGEEEASEVFKHLAPKEVQALGETI
                     AKMKAVTRDRVDGVLGKFSEMAGTQSMLVADNDEYVKSVLRRALGDEKANLLIDRILQ
                     GSDVSGIESLKWMDPQSVAELLRNEHPQIVAAIMVHLDYDQASSVLKLFSERQRNEVL
                     VRIATLDGIQPSALKDLNEVMSKVLAGGDKMRKASLGGVKPAAEIINMMGGSIETSVL
                     DYIREADNDLAQKIMDNMFTFDDLEKLEDKAIQTLMKEVQTESLVIALKGATPEMREK
                     VFRNMSTRAAETLREDLDSRGPVRVSEVEAEQKELLKIVRRLADEGQIQLSSGSDDDF
                     L"
     gene            21484..22236
                     /locus_tag="PKV56_07120"
     CDS             21484..22236
                     /locus_tag="PKV56_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOX67622.1"
                     /translation="MTSSKFKNVPPPEGGGKGSVYARFIPREELSSFEAWNPDALSGV
                     APRSGTGAPAVDPVPPAEALAEQLRAARQSGYQDGYRDGLSALDGFKQTYAKQITAQL
                     GRLLESHAAQLDGLQQQLAQSLAVCATSMARQVVRSELALRPELVAAVAQEAVDALLL
                     SARHITVRVHPDDQPLVAQGAAEVLDARGARLVSDASIARGGCRVDSDIASVDSTVPT
                     RWRRAAAALGPAAEWSDEPEEGAEPPAGEEQK"
     gene            22353..23702
                     /gene="fliI"
                     /locus_tag="PKV56_07125"
     CDS             22353..23702
                     /gene="fliI"
                     /locus_tag="PKV56_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOX67623.1"
                     /translation="MFTADPVPLEIQGMLVRVAGLVLESAGLREPVGSVCEVHMSGQP
                     PVQAEVVGFSGDRTYLMPTGNVHGLASGARVVPKASPTVPLLLGAARHPWRRSLDRTL
                     HLPVGDGLLGRVIDSQGHPMDRKGPLTDVHDEPLVRRPINAMDRDPVRRPLDTGVRAI
                     NSMLTVGRGQRLGLFAGTGVGKSVLMGMMARYTEADVIVVGLIGERGREVKEFIEDIL
                     GADGLARSVVVAAPADSPPLTRMQGANYATAIAEHFRDRGQHVLLLMDSLTRYAMAQR
                     EIALAIGEPPATKGYPPSCFAKLPQLVERSGNGLAGSGSITAIYTVLTEGDDPQDPIA
                     DAARGILDGHIVLSRELAEAGHYPAIDVERSISRVMTQVSPPQHVEAARRFRQLHAKH
                     QKARDLIQLGAYAPGHDSELDSAVRLHAPMTQFLQQDMHQPATLADSVRRLGAVMAG"
     gene            23751..24215
                     /gene="fliJ"
                     /locus_tag="PKV56_07130"
     CDS             23751..24215
                     /gene="fliJ"
                     /locus_tag="PKV56_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOX67624.1"
                     /translation="MNSLQPVMTLLGQTERERDELAADCQRASVAQRTASVQLEQLVT
                     YRREYEERWAHQFRTDGRMELVNCYRGFMERLTQAVEQQRRVAVQAGAELEQAQLALR
                     EGEIRVASVRKLIERRVADVRVAVDRREQKASDEFASRAAWMQRSNGLAGAL"
     gene            24263..25609
                     /locus_tag="PKV56_07135"
     CDS             24263..25609
                     /locus_tag="PKV56_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOX67625.1"
                     /translation="MNTAGVSTSSNPSAASARSAGEGQGGGPSDFSRLLVRSQAAADS
                     QRTVAPAGRTPPPQRPASPTPNSRPETPGPRAERDASDADASSGVAEGADTAACACAT
                     AEQPSERAGPLRSRRSAPAGKAPANAASAEVAGAEATSTWDARTPKAAPAAPAAAELA
                     SLAAWGALPMPAAAPPAANADSEGEIAAPDGTATSAASSEPASSSPIDARAAESMDAF
                     EASDRDAATRAMAQESPLALDASSLPAGGAAPDVAMVNMAMLSAGVRPELEADLGQGA
                     DLAIPPAWALGAAGSSTPSRAVDAAVPSASATVATPLGDSGFHEALGMQVSLLAREGI
                     QKAELRLNPADMGPVSVQITMNGDQARVDFGADLAQTRQVIEAGWAELAASLQEAGFT
                     LSGGGVSEHARDPRGAPQGMVPPSPVATASADGDQEPAASIIVPRPRAGAALDLYA"
     assembly_gap    25615..25714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25863..26537
                     /locus_tag="PKV56_07140"
     CDS             25863..26537
                     /locus_tag="PKV56_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOX67626.1"
                     /translation="MATAAATAPAAEAGDAPAPKKGKKKLIIIIAAVLLVVLAGGGAA
                     VYMMKQKAAAAEAAAAEAEGEDGEAAPAKDAHAKKDEHKGPPVFVPLDPFVVNLADRD
                     SERYAQIGITLQVDDPKFADELKLYLPAVRNGILMVLAHKTSKELLERSGKERLAAEI
                     MREAVRPMGIEIALEDPEADAQQHEAPADPDAEEAPKPKKKKKKKAPEVHNPVTGVNF
                     SSFIIQ"
     gene            26551..>26905
                     /gene="fliM"
                     /locus_tag="PKV56_07145"
     CDS             26551..>26905
                     /gene="fliM"
                     /locus_tag="PKV56_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520628.1"
                     /note="with FliG and FliN makes up the switch complex
                     which is involved in switching the direction of the
                     flagella rotation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOX67627.1"
                     /translation="MNQQILSQDEVDALLQGITGESQKLEAEDVPQGGIRNYDISSQE
                     RIVRGRMPTMEIISERFARNIRIGLFNMIRKSPEVSIGGIKVQKYSAFLREIVVPTNF
                     NIVSVKPLRGSGLIVC"
BASE COUNT         4617 a         8896 c         9090 g         4202 t
ORIGIN      
        1 agacaggaac caccggcctg cgttctggtg ccggggcgaa ttcatcatcc ggcagccgca
       61 gatcggcctg catggtgcgc gcgatgttgc gttcacgcgc cgtggtctcg accttcatga
      121 acgccactcg catgaaggcc gcgtgctcct gcgtgcaggg ctgcagcttc aggtccaggt
      181 aggtcgagtc gatcatgttg aagcggtgct cgaaccagcc caggtaccag aaccattccg
      241 tgtagtagat ccacgagttc tggttgagtg cgcgaacgtg ggtcgggtcc tgccacgctg
      301 tcagcgcgcg ctcgtagggc acctcgatgt cgaagcgtcc gccgtccttc agcagcgcaa
      361 gcgcgttggt catcagcagg ctcaggtcgg gcacgtgctc cagcacgttg ttggcataca
      421 gcctgtcgat cgttccggcg gccagttcga tctgtccgcc gtagcggttc tcgatgacca
      481 gcggcagcgt gatcggctga ccgatatcga gcaccgcgtc gggctctgcg cggtcctgga
      541 cgtcgaggtt gagccagccc ggcttgtagt ccttgcccga gccgaggttg atgtgcgagg
      601 ggcgccaggc gccggtgtcg ccggtgccca gtcgcgtgat gccgtggcct tcgagatagc
      661 cgcatgcaaa ccacagcagg tcggcatagc tctcgatcgg atcgtgcccg cggaacgagg
      721 ccagcttctg cccagcctcg ctggcatagg ccccggtgcc gaagtgcttc gtgaagaacg
      781 tctccagcgc atgaccggcc gggtcgatcc agaacaccga gtcggtgaac gccgccggca
      841 cgtgggcgtc ggcatggtgt tccgagatca ccggtgttcc catcgacagg cactggaaga
      901 cgcgtgcctg ctcgaagcgg ctggtctcgt agaagctgca gttgagcacc gccttcgact
      961 ggcggatgta ggcatcgcgc tcttcgccgt acagcgggtg gtcgaacatc gacaccgaga
     1021 cgccgcaggc ctcgatgcgg ttgatgaagg cgcgccggcg ctcgttcatg ctgccgaaga
     1081 agagcaggtc gatcggccgt tcggcgatcg gcatcgtgcc ggccgattcg aggtagggcg
     1141 catgcaggaa cggcagcacc ggcacctctg cggcgtctgc ggcgtaggcg ggaatgttgg
     1201 ctgcgtcgta gtcggcgacg gccgagccgc gcagcagctt gatgtagtca tcgctgaccg
     1261 gcgcgccccc ggagccgagc tgctccaggt tgaagaacac gcaggcgtgg cgacgacgcc
     1321 agtcgggcgg gaatccgaga tgcgcgccga acacgatgtt cagcgcgtcc tcgcgcacgc
     1381 ggttcttggc gatcgtcacc gtcgcgccga gacgctgcag ctggtaccgc acgtagcgcg
     1441 cctgatcgag caggccgagt gaatgaacgt agcccgcagg ctgcatcaac gtgatgtgga
     1501 tggggggcag gttcgtcatg gatcggcctc gatggacggc aaggtgccgc aggtgagtcg
     1561 atcctaggca gcgcccccgg gcatcggtgg gtcgatgaaa ccgccaaaag gccggcaatt
     1621 cttcgggaca tggcgcggga agtccgggcc cggaccgtac cggttggggc atatccccca
     1681 gttcagggtg taagccccca gctttcgccg aaccgttccg cggcagccgc ccagcgacgt
     1741 gtagggattt cttggagggc cggtcggcag gcggcgccaa tgcggcgtct acacactctg
     1801 taacggatct tgcccctcga tggcacagcc gttgcgatcc agaaggcata ttccggctcg
     1861 cccgaaggct gcttcaggca tccgaccagc ggaactgccc agggtcgccg gaatgagtcg
     1921 tggcaagtca tcagcaaccg catcgaatcc aggaagcacc ccatgagcaa agacagcagc
     1981 aaagaccgag actcttccga cgacaactcg accgtcagtg gctcatcgag caacgacggg
     2041 taccagttca ccatcgtggg cggcgcggtg acggcagtct tcgagatcga ggacggtgcg
     2101 acccgagcca agcagatgaa gtccaacgaa acctggacgg tggtcggcga tcaggtcgtg
     2161 aagaccgagg tcgagcatgg ctacaccgag gtctacacct ttgccgacgc caacggtgat
     2221 ggcatctacc tcaagatttc cgaagtccac accgacagca cgaccggtct gccaatctcc
     2281 aacggcgact tcggcgacga ctccagctcg agctggtctg gctcgggcgg ctccgagagt
     2341 caccacggcc gcggcggtga cgacgtgctc gagggcaatg cgggagacga tcgcctttat
     2401 ggtgaagacg gagacgacca cctgcacggc ggcgacgacg acgactacct gtcgggtgga
     2461 tcgggcatca acacggtggt cggtggcgcc ggcgacgacc tgatcgatgc caccggtggc
     2521 accggcaagg acctctacga cggttcggag ggtgtcgaca cggtcaacta cggagccgcc
     2581 accaaggggt tgaaggtcga cctcaacagc ggcagcgcca agggatcgga gttcggcaag
     2641 gacaagctga tcagcatcga gaacgtcgtc accggcagcg gcaacgacaa ggtctcgggc
     2701 agcacggcgg acaaccgcat cgaatccggc gcgggcaatg accagatcag tgccggtgat
     2761 ggcaacgact ggctgtccgg tggcctgggc ttcgacaagc tgaccggcgg gaagggagcc
     2821 gacaccttcg acttcaactc gctcgacgag ctggggctgg gcaaggcgac cgacagcatc
     2881 aaggatttcc ggcatgccga gggcgacagg atcgacctga gcggcatcga cgcaaacgcg
     2941 tcggtcgccg gtgaccaggc attcacgttc ctgtcggccg caccggtcga tggcgatcag
     3001 acgggtgccg tctggttctc gaagaaggcg ctgtacatca gcaccgatgc cgatgcgcag
     3061 gccgagttcc agatcaacct gtccggcgtg tccgcgctgg tggtcgaaga tctgattctc
     3121 tgacgcgacc accgcgcgtc tcgcactccc agggcctcgc cctgggagtt ttttttggtg
     3181 ggtgccgaat ccggcccgca ggtgtcgtgc cgaggcaccg ggcggcagcg ggagggatac
     3241 tctggccccg cccaggcgac atgtccgaat gaatccccct gtcaccccgt cggagctgcg
     3301 gtctgcggca gcagagttgc ggccgcagct ggttcgagcc accgcattca gcatcctggc
     3361 cggcctgctg gtgctggccc cgaccggcta catgttcgcg gtgtacgacc gtgtcctgaa
     3421 cagtcgcagc ccgacgacgc tggccatgct gaccctgctc gtgctcgggg cctatgtcgt
     3481 catggaggtg ctggagtggg cgcgtggcga actgctgcgt gaagccgctc tcgcgctcga
     3541 tcgccggctc tcgaaccggg tgttcgaggc gatcttcgag gcggaccgca gcaacctgcc
     3601 cggcggcacg actcagccga tgagcgacct gcgaaccctg cgcgagtttc tcaacgcgcc
     3661 ggtgctgccc gcgctgatgg agctgccggt gtcgctcgtg ttcctgttgc tgctcttcgc
     3721 catcagcccg ctgctcggcg ggtcggcgct cgtgggcgcc ctgctgcaga ccggtctgac
     3781 ctggctgagc gagcgcgcca cgcagccccc gctgctggct gccacgcgcg gcgcggcggc
     3841 cgcccagcaa tacgccgatg ccgccatgcg agatgccgaa gcgatcgagt ccatgggcat
     3901 gctcgatgcc attcaccgcc gctggagcgt gaagcagcgc gagttcctgc gtttgcaggc
     3961 acaggcctcc gatgcggccg gcggctacca ggcggcgtcc cggttcgtgc agcaggtgct
     4021 gggctcgctg ctgctcggtc tcggagcctg gctgctgctg cgcgacgaga ttcctggcgg
     4081 tgcgggcatg ctgatcgtcg ggtcgatcct cggtgcccgc gtgctggctc cgatggtgca
     4141 ggtcgtctcg aactggcgcc aggtggtgac ggtacgcgac gcctgggctc gactggatgc
     4201 actgcttgcg cagtggcccg cacggaccgc gtcgatgccg ttgccgactc cgcgcggcgt
     4261 gctgagcgtc gacggcctgc atgccggcgt gccgggcagt cccgccaaca tcctgcgcgg
     4321 tctgcagttc gccttgtcgc cgggggaggc gctggccgtg atcggaccct ccgcgtccgg
     4381 caagacgacg ctcgcaaggc tgctgctcgg gctgtggcct gcggccagcg gcaaggtccg
     4441 tctcgacggg gtcgatgtgc acggctggga caaggcagaa ctcggcccgc atgtcggcta
     4501 cctgccccag ggggccggtc tcttcgaggg cacgctggcc gagaacatcg cccgtttcgg
     4561 cgagatcgac atggcgcaag tcgaggccgc ggcgcgcggc gtcggtctgc acgacttcat
     4621 cgttgcgctg gaccggggtt acgacacccc ggtgggtgcc gacggcgcaa gactctccgg
     4681 tggccagcgc cagcgggttg cgctggcccg ggcactttac ggagagccgg tcttcgtggt
     4741 gctggacgag cccaattcca gcctcgacga agcgggcgag gccgcgctgg cggccgccat
     4801 cgcggcgggc aagcggcgcg gcgcgacctt cgtcgtgatc acccatcgca agagcgtgct
     4861 cgacgtggtc gacaagatgc tggtgctgcg cgatggggtg caacagcttt tcggcccgcg
     4921 cgacgaggtg atggcgcgct tcggctccgc cccgcaggcg gcccgcgcgt gatgatgcgg
     4981 atcgcgtcat gacgacctcc accgaacggg tgcctcgccg cgagctgccg cagacgctgt
     5041 ggtcgctgcg acgcgcgttc gcgctggtgg cggtgttcag cctggtggtc aatgtgctga
     5101 tgctggcgcc cacgctgtac atgctgcagg tctacgaccg cgtgctggcg agccgcagcg
     5161 agctcacgct gctggcggtg tcgctgatca cgctgttcct gctggcagtg atggcgacct
     5221 ccgaagccgt tcggtcgcgt gtgctggtgc gcgccgggcg gcggctcgac gagcggctga
     5281 gctcgcgtgt cttccatgcg agtttcgcgg ccggactggg cccgtcggct ggtgtgccgt
     5341 cgcgcgcctt cggcgacctg atcgaactgc ggcagttcct gacggggcca ggcatcttcg
     5401 cgctgtgcga tgcgccatgg tctccggtgt acatcgccgt gctgttcctg ctgcatccct
     5461 ggctgggtgt gatggcgatc ggattcgttc tgaaccaggc cgcgctggcc tggttcggcc
     5521 accggcacac ggtcgcaccg ctggccgtcg ccggcgaggc ccaggccgaa gccacggcct
     5581 acctgaagag caagctgcgc cacgccgagg tcatcgaggc catgggcatg ctggggcagt
     5641 tgcgccagcg ctgggctcgg cgtcaccaga accatctcgc gcgccagcac gccgcgctgg
     5701 ggctggccgg ccgcgtcacg gcatgcagca ggttcgtccg ctatgcgcag cagtcgctcg
     5761 cgctcggcgc gggtgcgctg ctggtgatcg acggccagtt gtcgccgggc gcgatgatcg
     5821 ccgcgaacgt gctgatgacg cgggccctgg tgccgatcga ccagctggtg tcggcctggc
     5881 ggggcttcgt cggcgcgcgc gacgccttcg cgcgactcgg caggttgctt gatgcccatc
     5941 ccgagcgccc tcgaatcgtc gccactgcca ttccttccgg tgccgtccgt ctgtccgacg
     6001 tggtggcacg ggcgccggga cgcgccgcgc cgctgctcga tggtgtcagc ctgtccgtgg
     6061 ctgccggcac cgtgacggtg gtgctcgggc cttcaggatc cggcaagtcc acgctggcgc
     6121 gggtgctggt cggcctgtgg ccctgcgatt ccggaagcat gctgctcgac ggcctgccga
     6181 tcgaccactg gagccgctgc gaactgggtc cgcacatcgg gtacctgccg caggacgtgg
     6241 agctgttcga gggttcgatc gccgagaaca tcgcccgttt cggcgaagtc gacgcggccc
     6301 gcgtcatcga ggcggcccgc gcggcgggtc tgcacgagac gatcctgcgc tttccgaagg
     6361 gctacgacac gccggtcggt gccgagggcg gactgctgtc gggcggtcag cgccagcgca
     6421 tcggactggc ccgtgccctg catggagatc ccgcgctggt ggtgctcgac gagcccaacg
     6481 ccaacctcga tgacgccggc gaggccgccc tgctggcagc ggttcaaggc tgccgggagc
     6541 ggggccggac cgtgttcctg atcagccacc ggcccggcat cgtcgaggcc gctgatcgcc
     6601 tgctgatcct gcgcgagggc cgcgttcaga tcgagggtcc gcgcgaggcc gtggtggccg
     6661 cgctgaaatc ggcgcagccc ccggcgatcc accccgcacc tctgaggccc gaacccgtga
     6721 gctccccgtc atgaatgtcg acgcgtcttc ctcgccctcc cggatggctg gcaccgaccc
     6781 gcaggacccg ccgctgtcgt cgggaacgcg tcgcgccgcc cggatcggtt ggtgggcact
     6841 cggtgccggc ctcggcgcct tcctgatatg ggccgcgctt gcaccgctcg acgagggcgt
     6901 gccgatgccg gggcaggtga gcctggacac caagcgcaag ccggtgcagc acctggccgg
     6961 cggcatcgtc aggcaggtga tggtgcacga gggggcgcat gtcgacgccg ggcaggcgct
     7021 gatgacgctg gtcgagacga ccgcacgcgc gaaccacgag gcggtgcgac agcgctacct
     7081 cgggctgcga gccgtgcagg gccgcctgct cgccgagcag gccggccaga ccgcgatcga
     7141 gctgcatccg gacctgatcg ccgcggccga tgacccctcc gcccaggcgc agatctcgac
     7201 ccagcaccag ttgctcgctt cgaggcgact gtcgctgcgc gccgacctgc aggcgatcga
     7261 ggagaacatc cgcgggcagg aagcgctgct gcagtcccat gcggcgatgt ccgcgcagcg
     7321 gcgcagccag caggtctcgt tgaccgagca gctcgacgcg atgcgcgggc tggtcacgga
     7381 gggatacgtg ccgcgcaacc gccagcgtga gctggagcgg caggtcgccg atgccgaggc
     7441 ggcgctggcc gaaatgaccg gcaacagtgc gcgtgtccgc cgcaccatgg ccgagctggc
     7501 ccagcgtgct gtcgtgcgtc agcaggcgta ccgcgaggaa gtcgacaccc agctggccga
     7561 cgtgacccgc gaagtgcagg gcgacgccca gcgcctggtg gcgttgcagg ccgagctgga
     7621 tcgcatggcg atccgcgcgc ctgcgaccgg gcaggtggtc gggctggtcg tgcagaccgt
     7681 cggggcggtg gtgcagcccg ggcagacgct gatggacatc gtgcccgacg gtcagccgct
     7741 gctgctcgag gcgcatgtcg cgccgcacct gatcgacaag gtgcgcgtcg gcttgccggc
     7801 cgacatccgc ttccagtcct tctcgcaggc gcagcagccg gtcgtcgagg ggcgtgtgga
     7861 gtcggtgtcg ggcgatctgc tgaccgatcc gcagaatccg cagcgcactt attacctcgc
     7921 tcgtctgagc gtgacgcccg cgggcctgaa ggcgctcggc gaacgccgcc tgcaacccgg
     7981 catgccggcc gacatcgtcg tcaggaccgg cgagcgcacg ctgctgacct acctgctgaa
     8041 tccgctgacg cggcgcgtcg cagcgtcgat gaaggaggag tgatcgatgg caccggtcac
     8101 tgacacgcga gcagcctgcc gtcgctcgtt cgcccggtcg gccaccctgg ccgcggccct
     8161 gtttatcagc ctgccgtcgc cggccatgga tctcagggaa gcctacctgg cggcgctcga
     8221 ccgggacgcc accctgcggg tgtcgcgcgc tgcagcccgc gtgggccagg agcgcctgcc
     8281 gcaggcgcgt gcgcagatgc tgccgaacct gtctgccagc atcggtcaca cccgcaacga
     8341 cctcgatttc agttcgcccg acccggtcac cggacgctcg cgccagaacg acagccgcta
     8401 caacagcaac aaccagacgg tcacgttgcg ccagccgttg atccgaccgt tccagtgggc
     8461 acagctccgg caggccgagg cgcaggtggc cgatgcgaac gcgaagctcg acaacgagct
     8521 gcagaacgtt ggcgtgcgcg ttgccgaggc gtacttccag acgctgcagg cggatgacga
     8581 actggcgctg gtgctggccc agaagaccag cttcgaggcg gagctcgacg cggcccgcaa
     8641 gtcgctggcc gcgggtgtgg ccacgcgggt cgggatcgag gatgcgcagg cccgcctgga
     8701 catggtggcc gcgcaggagc tcgaggcgcg gcagcagcgg gatcacgccc gcctccagct
     8761 ccagacgctg gtcgatcgtc cggtcgatca actcgcgccg ctcgatctgg atagcctcca
     8821 gctgttgccg ccggagccgg cgcgcgttga cgactgggtg gagcgtgcac tgcgcggcaa
     8881 tgcggaaatg cgcgcccttc aggcgcagct ggaggccgcc cggcaggaag tcgacaaggc
     8941 ccgtgcgggt cactacccga cgctcgatgc gacggcccag tggtcgcgca gccagagcga
     9001 gaacgtgctc aacaccgcgt cgcgatacac caacgtggcg gtcggtgtgc agctcaatgt
     9061 gccgatcttc gcgggcgggc aggtggattc cagcgtgcgt caggccctcg ccgagcagga
     9121 tcgggtcgag gcctcgctcg acgcgatgcg ccgcgatctg ggcttgcgcg tgcatcaggc
     9181 gtaccgatcg gtgaccgaga gcatccttcg ggctcgtgcg ctggagcagt cggtgcgcgc
     9241 agccgaccac ctggtgcgat cggcgcagaa gtctttcgag ggcggcagcc agacgcgcct
     9301 cgacgtgctc gatgccgaac agcggcgaac cggcgcgttg cgcgatctcg cgcgggcgcg
     9361 ctacgtgtgt ctggtgtcgg gcatccggct gcaggcgctc gtcggtggcg agcgcgatgc
     9421 cgccatcgat gcagtgaacg ccagcctgaa acgctgacga atcagcttgc ggctgcgagt
     9481 gcctgcggcg gcaggccctg gcgcgatcga tccaccattc ggcggtagag cgcttcgaca
     9541 tcctgcgcgc agcgggagcc gtcgaacagc gggaacacgg accgaccgcg gtcgagatgc
     9601 tcgcgcagtg cccgcagttt ctcgggatgc cgcaacaggt gaatgacgcc ggcctcgtat
     9661 tgcgccacgt tctcgtagac caggctgtcc agtgccaccg catgcagcag gctggctgcc
     9721 acgcgtgatg cgaacgagtc accgatcacc gtggccagcg gcacgcccat ccagagggcg
     9781 tcgctggcag tggtgtgggc attgcagggc cagttgtcga gcatcaggtc ggccagtggc
     9841 aggcgcgcaa ggtgctcgcc gacgctgacc ttggccgcga agatcaggcg ctcggggtcg
     9901 acgccgcgtt gctgcgcttc gcgccgcagg ttcgcggtcg ccagcgggtt gtcgttcagc
     9961 agccagagca cggcattcgg caccgcacgc aggatgcgca tccaggcatc gaacgtctgc
    10021 ggcgtcagct tgaagctctg gttgaagcag cccaggacca gcgcctgctt cggcagcccc
    10081 cactgcatcc gctgcgacgg cgccggccgg ggccgctggc tgtcgttggg ctggtagctg
    10141 tgcgggagtt gggccaggca ctcggtgtag tgcgccgcgt gcgacagcgg ggtcgcgacc
    10201 gggtcgccga tgaagtagtc gatgcagtgc atgcctgtcg tgccgggaaa gccgagccag
    10261 ctgacctgca cgggcgcagc gcgataggcc aggaccgcca ggcgactgcc ctgcgtgtgg
    10321 cccttcagtt caaccagcac gtcgatgccg tcggcacgga tgcgctcggc catctcgcga
    10381 ggccccatcg cgcgcatgtc gacgaaatgc tcgcaggccg ctcgcacgcg atgctgcatc
    10441 gcggtgtggt cgtcgtggcc atatgaatag agcgtgacct cgaaatcgcg cgtgttgcgt
    10501 ttctccagcg tctcgaccag cagctgcgcg gtggcgtggt tgtggaagtc gcacgacagg
    10561 tagccgatgc gaatgcgacc agccgagtcg cgttctcgtg tctgcggcgg caggggcagc
    10621 acgcggccgg tgtggaacgc cgctgcctgc tgcgccgcga ccttgaacag cgcgggcggg
    10681 tgggggatgc ccagcaacgc gaaggtggcg ccctcgccgt gttcgggctg ctcgggattc
    10741 agccgtgcca tcgccgcgac ggcttcgctg cagctggcgt cgaagtcccg ccagtcgcag
    10801 gcgtactggc gcatgtggat cgccatcagg cgcgccgaca ggcgatccgg ctgcagcgcg
    10861 acggtggtca ggaagctctc ggccgcctcg tcgtacatct tgaggtcctt gaggaccagg
    10921 ccgatgcgca ggtgcacgct ggggtcgctg atattcagtg acagtgcgcg caggaagaca
    10981 tccagggact ccttcagccg gcgcagctgc cagagggcgg tgccctgatt gaccaggaag
    11041 ctgaacgatt cctgggccgc ctcgagatgg ccatggctct cgaacacctc gaccacttcg
    11101 gaccagcggt gctgctccat caaggcgatg gtcagcacct gcaccacgcg gaaattgtcg
    11161 ggatcctcgg ccagcgcacg ccgtgcggcc cgtgctgcat cgaccagcga acccgcatgg
    11221 atgctggcga tggcgagaga cagccagtcc ttcgccgaga agcgggcgtc cgtggaaagt
    11281 gcgcccaggt cctgcgcggc gccgacccag tcgtgcttcg tgacgtgctg ataggcgttg
    11341 cgcttggcag tgagaaaggc gcgttcggat tgcattcggt tgccgatggg tgagtgcgtt
    11401 cgtcgggccg ggggtgctgc ggatccgggg cgcatgcgaa ctgcccaact cgcgtgaatc
    11461 taggggcgac aggcgcgggc gatgcgttga aaagctctcg acccaccgtg cttttctcga
    11521 ggatgttggt tcgggttctc caaatttcct caagttgcaa agtgatctga cgaaaaacat
    11581 ggcaacgagg cgtcagtccc aagcctcgtt gtccggaaca gcagaccgca ggtccccgta
    11641 acaaacgatc aggggttttg caggatgact gccgccgggc gcagccggtc cagggagtcc
    11701 cggaaagtgc ctaggcaccg cccgcccgaa gactggctcg atagaggtgg ggcgacccgc
    11761 ctccatcgag gcaaagccgg cgtccacgcc gggagttcat caacataagg agctttttca
    11821 tggcaatgac catcaacacc aatgtggcct cgctgaccgc gcaacgcaac ctgagttcgt
    11881 cgcaaagcgc cttgtccaca tccatgcagc gtctgtcttc cggcttgcgc gtcaacagcg
    11941 ccaaggacga tgcggccggc ctcgcgattg ccgagcgcat gaacgcaacc acccgcggcc
    12001 tgaacgtcgc tgcacgcaac tcgaacgacg gcatctcgct ggcgcagacc gctgaaggtg
    12061 cgctcggcaa ggtcggcgac atgctgcagc gcatgcgtga actggcagtg caatcggcca
    12121 acgccaccaa cagcgctgac gaccgcacgg ctctccagtc cgaagtcggc cagctgcgcg
    12181 atgaaatcaa ccgcgtcgcc aagcagacgt cgttcaacgg caccaagctg ctcgacggtt
    12241 cgttcgcagc cgcgacgttc caggtcggcg ccggcgccgg cgagaacatc acgatcgccg
    12301 ccttgacgaa tgcgtcgtcg gaccagctgt ccaacgtcac ctacggctcc ggcgatgtca
    12361 cgggcatcag caccggtacc gtcacgaccc tgaccgaagt ggccgccggc acgctggcga
    12421 tgaccgtcga cggcaccgag tacgaactgt cggccctcgg cgccgcgaac tccgcagagg
    12481 aacgcctggg tcaggtcgct gaagcgatca accgccgctc ttccgacacc ggcgtgaccg
    12541 cctacctgtc caaggatgcc agcaccggcg actacgacct gaagttccgc tcgggcaacc
    12601 tggattcgtc cgacgcagcc gtggccgtgt cgttcggtgg cttcaccgtg gccgagaccg
    12661 gcatcggcga caccagcgcc acgatcgcct ccaccgcggt caccggcttc gacgacctgt
    12721 cggtcgagac caccgaagat tcgtggctcg ccatcaagca gatcgacgac gctctcgacc
    12781 aggtcaacgg tgcacgtgcc acgctgggtg ctgtccagag ccgcttcgag aacgcggtgt
    12841 cgaacatcat gatccagtcg gagaacacgg cagcggcccg cggtcgaatc gtcgatgcgg
    12901 acttcgcgtc ggaaacggcc aacctgtcca agtcgcagat cctgcagcag gccggcacgg
    12961 cgatggtggc ccaggccaac cagttgccgc agcaggtgct gtcgctgctg cgctgatctc
    13021 gcggcaggca gactggggcg gcgaaagccg ctaccactgt ggtttcacca ggcctcgtcc
    13081 ctccggacgg ggccttttgc tttgtggtcg cgataacttg cattgaacgc tcgtgcatcg
    13141 aggattggcc ggattaacag gcgcttatca gacgatggcg tcgtgcgccg ctgccgaaga
    13201 atgaggacat gaacagcagt cctttgggct gcgtcggtcc cggcaacccg ccggatctcg
    13261 gtcccgatga ctttggagag cggccatgcc acagacgatc aacacgaaca tcaattcgct
    13321 gactgcgcag cgcaacctca atgcatcgca gggctcgctc tcgacatcga tgcagcggct
    13381 gtcctccggc ctgcgagtca acagcgcgaa ggacgacgcg gccggtctgg cgattgccga
    13441 gcgcatgaac acccaggtgc gcggcctgaa tgtcgcggcc cgcaatgcca acgacggcat
    13501 ctcgctggcc caggtggcgg aaggcgccct cggcaaggtc ggcgacatgc tgcaacgcat
    13561 gcgtgaactg gcagtgcagt cggccaacgc caccaacagc gcagacgacc gcaaggcgct
    13621 gcagtccgaa gtcggccagc tgcgcgatga aatcaaccgc gtcgccaagc agacgtcgtt
    13681 caacggcacc aagctgcttg acggcagctt ctccgcggcg acgttccaga tcggcgccaa
    13741 ctccggcgag gcgatcacca tcgctgccct gaccaacgca tcgaccgacg gcctctcgaa
    13801 cgtcgattac ggtgcgggca acgtcaccga cgtgagcacc ggaacgatca cgacactgac
    13861 cgccgtggcc gccggtaccc tggccatgac ggtcgatggc acggagtacg acctcgctgc
    13921 tctcggcgat gcacgcagtg ccgaagagcg cctgggccag gtggccgaag cgatcaaccg
    13981 acgcgcgtcg gacaccggtg tcaccgccta cctctcgaag gagacggacg gcgactacac
    14041 gatcgagttc cgctccagca agcttgattc caccggcgtt ccggcggcgg tttcgttcgg
    14101 cgggttcacc gttgccgaga ccggcatcgg cgacaccagc gccacgatcg ccgcgaccaa
    14161 cgccaccggc atcgccgacc tgtcggtcga gacgaccgag gaatcgtggc tcgccatcaa
    14221 gcagatcgac gactcgctcg accagatcaa cagcgcacgc gccaccctgg gtgcggtgca
    14281 gagccgcttc gagaacgcgg tgtcgaacat catgatccag tcggagaaca ccgctgcggc
    14341 acgcgggcgg atcatggatg ccgacttcgc ggcggaaacc gccaacctgt cgcgcgccca
    14401 gatcctgcag caggccggca cggcgatggt ggcgcaggcc aaccagttgc cgcagcaggt
    14461 gctgcaactg cttcagggct gatccccgtt cgcgaagctc gccggtcgca gcgacccacc
    14521 cgggcgggtt ccctggtggg tcgttctcat tggggcatcg gtcgccgtgg cgcaaacgcc
    14581 tgcggacccg gggcctcaag atttcccgca aggcgccgat agacaccgaa gagcacagga
    14641 gcgtgatcca tggcgaccat ctcatccccc ggcctgggca gtggcctgaa cgtgaccagc
    14701 atcgtcgatc agctggtggc cgtcgagcgg cgaccgatcg acctgctgac cactcagaag
    14761 accgcgctgc agtccaagct gtcggcgttc gggctgctga agagctacac caccaacgtc
    14821 gaggacgcgg tcgccagact ggcggacccg acgctctggc agaagacctc gaccgggagc
    14881 accgatcctt cctcggtgac cgcgacatcg acggccgcgg cgaaggacgg cagctacagc
    14941 gtccaggtgg gacagctcgc gcaggcccag tcgctggcgt ctggggccta cgccagcaac
    15001 agcgcagcgg tcggcaccgg aaccctgaag atcgaactcg ggacctgggg tgccggcccc
    15061 gcattcgccg acaaggtacc ggcgtcgtcg gtcgacatcg tgatcggtgc gggcgacaac
    15121 acgctggaag gcgtgcgcgc caagatcaac gccgccaatg ccggcgtcag cgcgtcgatc
    15181 atcaaggacg gcagtggctc gcgcctggtg atccgctcgc ttgccacggg ggccgagaac
    15241 gcgatgcgca tcaccgccac cggggccgcc gcgctgccgg tgctgccgag cgatccgccc
    15301 tcgctggcca gtctcgtcta caacccggcg gttccggcga gcagcaagtt gacccaggcg
    15361 gtcgaagcca aggacgcgca ggcgacgatc aacgggttgg ccgtcacctc caagagcaac
    15421 acctttgccg acgtcctcga aggcgtcacg ctgaccgtca gcaaggtcac gagcaccgcg
    15481 gcgcaggtga gcgtgggaat cgacaaggac tcgatgaaga aggccgtggc cgacttcgtc
    15541 aaggcctaca acgacatcag caagtacctg gcccagcaga ccaagtacga cccggatacc
    15601 aagaccgccg gtgaccttca gggcgaccgt gcgaccctgt cgttgcagag ccagctgcga
    15661 aatggattga tgcagtccag cggcgcatcg accgtctttc gcagcatgtc cgacatgggc
    15721 atcgagctgc agcgagacgg cacgctcacc gtcaatgaca gcaagctcgg caccgcgttg
    15781 agcaatccga ccgaggtcag caaggcgttc acgagcgcag caaccaacgg gcccggctac
    15841 accggattgg cggtcaggct gaagagcctg acggattcga tgacgctgac cgatggcctg
    15901 gtgaccacgc gctcggacgg ccttcgaacc tcgatcaagc gtgtcgatga ccaggtcgcg
    15961 aagtacgagg aacggattgc cgcgaagagg gaaagcctgc tcaggcagta cagcgcgctc
    16021 gacaccaagc tcagctcgct gaccagcctg aactcgtaca tatcgcagca gatcaccaac
    16081 tggaacaaga gcaccaactg acggcattct cgatgccatt caggacggtt gttgcctcaa
    16141 gtgcgggcca cccaggccga taaaaaaagc acacagactt cttgaggatg ccagcgatgt
    16201 ttgccgccgc caaccccttc cggaccccca gtttcaacgc gcagtcgaat gcctaccggc
    16261 agatcggcgt gacctcggcg gtcgatggtg cgaacccgca ccgcctggtg ggcatgctgt
    16321 acgaaggact gctggagtcg atcgccgaag cccgtggcgc cctgcgcacc ggcaacgtcg
    16381 aaatgaaatg ccgcagcgtc agccgtgccg tgcgcatcgt cgaggaaggc ctcaagggcg
    16441 gcctcgatct gcaatcgggt ggcgagatcg ctgcgaacct gagcctcgtc tatgggtacg
    16501 tttcccagcg cctgacgatg gccaacctgc agaacgacga cgcgatgctg cagcaatgcg
    16561 ccgaactgat ccagccgctg cgcgaagcct ggacacagat cggcgccgcc gtggcgaccc
    16621 agcccctggt cgtcaacgga tgaatctgca accgcacccc atctcacgcg tacaccccat
    16681 gacacagaac ctgctccagt actacgaagc catcgaacgc gccagccagg acatgctgga
    16741 ggccgctcgc aatggcaact gggatgccgt cgtgaagctc gaaggcgcct gtgcgctgct
    16801 gatctcgcag ctgaagaacg ccgctgccga tcgcaagctg gccgccgacg aggcccaatt
    16861 gaagtcgcgc atcatgcagc gcatcctcgt caacgatgcc gaggtccgcg tgctggccga
    16921 accctggctc gagggactgg acgatctgct cgccggtcgc tcggccacct tgcattgaac
    16981 accgggccga cgatggacac cctgcctatg ccgatggaca cgatgcccat gccgatggac
    17041 acgctgccta tgccgctcga tgcggtggca gcggctcacg gcggcctcga tgatttccgc
    17101 atcactgcgc catccgaact ggcgacgatg ctcaagcgtc tcctcgatgc cggggtggtg
    17161 atcaacctga acgccccgaa cggtacggcg gtggcgtcgt tcctgtggac ggtcgatgcg
    17221 gcgcgtggcg cgatcacctt cagcgcggat gcccatgcac cccagttgca ggcgctgatc
    17281 gaatgcaatg aagcggtggc ggtcgcctac ctcgacagca tcaaggtgca attcgacgtg
    17341 cacggactga tgctggttca caacggacgc tccagcgcga tcagctgcgg ctacccgcgc
    17401 gagatattcc gcttccagcg acgcaatggc taccgtgtca ggccgctgct gcgaaacgcg
    17461 ccgacggcac gcctgcggca cccgatgatc cccgacatgc agctcaacct gcgggtgctc
    17521 gatgtgagca tcggcggttg cgcgctgttc ctgccggaag atgtgccgat gctcgagccg
    17581 ggcgtgctgc tgaacggagt ggacctcgat ctcgacgccg acacccacat cgtggcaccg
    17641 ctgcgcctgc agcacgtgac ctcgctgcac ctggaggcac gcggtgtgcg tctgggctgc
    17701 gagctggtca acgccagcaa cgagagcctg cgggccctgc agcgctacat cgaccagacg
    17761 cagaaacgcc ggcgtctgat ggttctcgac tgaggggcag ccaggccctg cagacggtcg
    17821 agcgccacgc acagcgtgcg ccagttcaca ctttgtgaca aatgttcggt cccgggctgt
    17881 caagtccgga tcaatgacgc cgaaaccctg aagacaggca accccgcccc gatctcagga
    17941 gcgattcatg agcgtcagta gcatccctgc agtcagtcgc gtcgaaccag cggcctcctc
    18001 ggcacgcccc tccgacactc cccaacgacc tcagagcgaa cagtcgtcgc ctgccgtcgg
    18061 ccacaagttt ctcgaagagc ccgaaccgcg ccgcttcccc tggctgagct gggaaacgcg
    18121 cgaactcgag gtcgcttcga agcaaccctc cccgtacggc aacatcccgc tgctgggcaa
    18181 ggaactcgac cggcaggtct gatcgacgat gcgcagggtc ggctcacgcc tacacctgca
    18241 tgttcatgat gtcgctgtag gcctggacca gcttgttgcg cacctgaagc gtggcctgga
    18301 atccgatctg cgccttctgc atcgcgacca tggtctcctc gatgctgaca ttcgggttgt
    18361 ccagttgcac ctcgcgctgc atccggctcg cctgtgtctg gctgttgctg accgactgga
    18421 tcgcctgcgt caacgccgcc tggaagccac cgtcctcggt gggctgcacc ttcttcagcg
    18481 gttgtccctg ggtgttcaga cccgcccggg ccaccgcctg agcgaagtcg aacggcttca
    18541 gcgtgacatt cattgcgacc cctttcaagc accctgtgcg ccgggcagct tcctgccacc
    18601 cgcacgggat gaatcgttga cggcaatcgt agccagacgg cctcggcgcg atggcgcgaa
    18661 ctggccgggg tttggtgggc tgttcggtcc ttggatcggg gtccggcgtt cgaataatgg
    18721 tcgctatcgg ccggcacttt cggccccgca tccgacttga acgagaacga tggacaacgc
    18781 agtcaccctg aattcacctg gcgccgtggc caccgcacca ggtttcggca gccgcctggc
    18841 cgccttgccg gcaaagagca agttcaacct tggcatgggc gccgccgcat tggtcggcat
    18901 cgtggtggcc ctgctcctct ggagcaacca gggcgactac aaggtcctct atgcgaacct
    18961 gtccgacaag gacggcggcg cgatcattgc gcagctgtcg cagatgaaca ttccgtaccg
    19021 ccattccgat ggcggagcgg cgatcctggt gccggcctcg aaggtccatg acgcccgatt
    19081 gaagctcgcc tcggccggcc tgcccaaggc gtcggtcgga gggttcgaga tgatggacag
    19141 cgcccgcttc ggtcagacgc agttccagga gcgcctgaca ttccagcgcg ggctcgaagg
    19201 cgaactgagc cgctcgatcg gctccctggc cgccgtcgag agcgcacgcg tgcacctcgc
    19261 gctgccgaac cagaacggtt tcttccgcga acagcagaag cccagtgcct cggtggtgct
    19321 gtcgctgcat gccggccgca cgctcgatcg gggtcaggtg gctggcatcg tgcatcttgt
    19381 gtcaagcagc gtgcccgaga tgagccccaa ggccgtcagc gtgctcgacc agaccggtgc
    19441 cttgatctca tcgccgcccg atgtgggaca gcaggccgga ctcgacgcac agcaactgca
    19501 gtacgtcgcc caggtcgaac agggttacag caagcgcatc ttcgagttgc tcgagccggt
    19561 ggtcggtcgc gagaacctgc gggccaacgt gaccgccgac atcgacttcg cgcagatcga
    19621 gtcgaccgag gaggccttca agccgaacca gggtgccagt gccccggcca cggtccgcag
    19681 ccagcagacg accgagcagt ccggcagcaa tccggcgcag gccgcgggcg ttccgggcgc
    19741 aaccagcaac cagccgccgc tgcccgccgc ggcgcctgtc accggtgccg cagcaccgct
    19801 gcagaccgcg cagaacggtg gcgccgtcgg caacagccgg cgcgaggcag tgacgaattt
    19861 cgaggtcgac aagacggtgc gtgtcacccg caacgcgacc ggcaacatca agcggctgaa
    19921 tgccgcggtg gtggtcaaca accgcagcgt caccgacccc aagggcaagg tcacgcaggt
    19981 accgctggcg ccggaggagc tcgagaagct gaccgcgctg gtgcaggaaa gcatcggctt
    20041 caacaaggaa cgtggcgact cggtgcgcgt catcaacgcg ccgttcaagg tcgaacccat
    20101 caccgtcgtc gacacgccga tctggaagca gcccgagatc atcgacatgc tgcgcgcggc
    20161 cgcggtgccg gccggtctgt cgctggtcgc gctgctcgtg ttcttcggaa tgatcaagcc
    20221 cggcctgaac gcggcgcttg cgccagccgt gccgctgccc ggcagcgcga tcgacgccag
    20281 cgtcgacgat gtcgaagtgc tgcctgggtt gccgcagggc gaggtccggg cgctggaggc
    20341 gccgcgcaat accgagtccc tcgacgcagc gcgcgcgctg gccaaggaaa acccggcggc
    20401 cgtcgcgcag atcgtgcgtg gctgggtcag tggccagccg gcctgatccc cgcgcgccag
    20461 acgaacccca ttgaaccgat gaccccacga tgagcgccga atccaacgaa caaggcctcg
    20521 aggacgcggc gatcctgctg atgtcgctcg gcgaggaaga ggcgtccgag gtcttcaagc
    20581 acctggcacc gaaagaggtg caggcgctcg gcgagaccat cgccaagatg aaggccgtga
    20641 cgcgcgaccg cgtcgacggc gtgctcggca agttctcgga gatggcggga acgcagagca
    20701 tgctggtcgc cgacaacgac gagtacgtca agagtgtctt gcgccgtgcc ctcggagacg
    20761 agaaggccaa cctgctgatc gaccgcatcc tgcagggcag cgatgtctcg ggcatcgaga
    20821 gcctgaagtg gatggatccg cagtcggtgg ccgaactgct gcgcaacgag cacccgcaga
    20881 tcgtcgcggc gatcatggtg cacctggact acgaccaggc ctcgtcggtg ctgaagctgt
    20941 tcagcgagcg ccagcgcaac gaggtgctgg ttcgcatcgc cacgctcgac ggcatccagc
    21001 cttcggcgct gaaggacctc aacgaggtga tgagcaaggt gctggccggt ggcgacaaga
    21061 tgcgcaaggc ctcgctgggt ggcgtcaaac ctgccgccga gatcatcaac atgatgggcg
    21121 gcagcatcga gacctcggtg ctcgactaca tccgcgaagc cgacaacgac ctggcccaga
    21181 agatcatgga caacatgttc accttcgacg acctggagaa gctcgaggac aaggcgatcc
    21241 agacgctgat gaaggaagtg cagaccgagt cgctggtcat cgcgctcaag ggcgcgaccc
    21301 ccgagatgcg cgagaaggtg ttccgcaaca tgtcgacccg cgcggccgag acattgcgcg
    21361 aagacctcga ctctcgcgga ccggtgcgtg tcagcgaggt cgaggcggaa cagaaggaat
    21421 tgctgaagat cgtccgccgc ctggccgacg aaggccagat ccagttgagc agcggcagcg
    21481 acgatgactt cctctaaatt caagaacgtg ccgcctccgg aaggcggtgg caagggatcg
    21541 gtctacgcgc ggttcattcc gcgcgaggaa ctctccagct tcgaggcctg gaatcccgat
    21601 gcgctgtcgg gcgtggcgcc gcgcagcggc actggagcac ccgccgtcga ccccgtgccg
    21661 ccggccgaag cactggcgga gcagcttcgt gcggcgcgcc agagcggcta ccaggacggt
    21721 taccgggacg gcttgtccgc gctcgacgga ttcaagcaga cctacgcgaa gcagatcacg
    21781 gcgcaactgg gccggctgct ggagtcgcac gctgcgcagc tcgacgggct gcagcagcag
    21841 ctcgcccagt ccctggccgt ttgcgcgacg tcgatggcgc gtcaggtcgt tcgcagcgaa
    21901 ctggccttgc gaccggagct ggtggcggct gtcgcccagg aggccgtcga tgcgctgctg
    21961 ctcagcgccc gtcacatcac cgtgcgagtg catcccgatg accagccgct ggtcgcccag
    22021 ggcgctgccg aggtgctcga tgcgcgcggt gccaggctcg tcagcgatgc gtccatcgcg
    22081 cgaggcggct gccgcgtcga cagcgacatc gcgagtgtcg acagcaccgt tccgacgcga
    22141 tggcgccgtg ccgctgcggc gctcggcccg gccgccgagt ggagcgatga accggaggaa
    22201 ggcgccgagc cgcctgccgg cgaggagcag aaatgatcac cagtccgagc gagaacgacg
    22261 cgctgcagcg cgcggcgcgt gcagcccagg tcgtccagca ggcgccgggc ggtcgggctg
    22321 atcaatggac ccgatacctc gacgcactga agatgttcac tgcggatccg gtgccgctgg
    22381 aaatccaggg catgctggtg cgcgtcgcgg gcctggtgct cgagtccgcc ggactgcgcg
    22441 agccggtcgg ctcggtgtgc gaagtgcaca tgagcggcca gccgccggtt caggccgagg
    22501 tggtcggctt ctccggcgat cgcacctacc tgatgcccac cggcaacgtg catggacttg
    22561 ccagtggcgc gcgcgtggtg ccgaaggcgt cgccgacggt gccgctgctc ctcggcgcgg
    22621 cacgccatcc ctggcggcgc agcctggacc gcacgctgca cctgccggtc ggcgatggcc
    22681 tgctcggccg cgtcatcgat tcgcaggggc atccgatgga ccgcaagggc ccgctgaccg
    22741 acgtgcacga cgagcccctg gtgcgccgcc cgatcaatgc gatggaccgt gatccggttc
    22801 gtcgcccgct cgacaccggc gtgcgcgcca tcaattcgat gttgacggtg ggccgcggcc
    22861 agcgcctggg attgttcgcc ggcaccggtg tgggcaagtc ggtgctgatg ggcatgatgg
    22921 cccgctatac cgaggccgac gtgatcgtcg tcggactgat cggcgagcgt ggccgtgaag
    22981 tcaaggagtt catcgaggac atcctgggtg cggacggact ggcgcgttcg gtggtcgtcg
    23041 ctgcgccggc cgattcgccg ccgctcacgc gcatgcaggg tgccaactac gcgaccgcga
    23101 tcgccgagca tttccgcgac cgcggccagc atgtgctgct gctgatggat tcgctgacgc
    23161 gctatgcgat ggcacagcgc gagatcgcac tggcgattgg cgaaccgccg gccaccaagg
    23221 gctacccgcc gagctgcttt gccaagctgc cgcagctggt cgagcgcagc ggcaacgggc
    23281 tggccggcag cggttcgatc accgcgatct acaccgtgct gaccgagggc gatgatccgc
    23341 aggatccgat cgccgatgcg gcgcgaggca tcctcgacgg ccacatcgtg ctctcgcgcg
    23401 agctggcgga ggcaggccac tacccggcga tcgatgtcga gcgttccatc tcccgcgtga
    23461 tgacgcaggt gtcgccgccg cagcacgttg aagcggcgcg gcgtttccgc cagctgcacg
    23521 cgaagcatca gaaggcacgc gacctgatcc agctcggcgc ctacgccccg ggccacgaca
    23581 gcgaactcga cagtgcggtt cgactgcacg cgccgatgac ccagttcctg cagcaggaca
    23641 tgcaccagcc cgccacgctc gccgacagcg tgaggcggct gggcgcggtg atggccggtt
    23701 gacccactgc tcattgaatc tactgaaccc ctgaagcgaa ggctttcgtc atgaactcac
    23761 tgcaacccgt gatgaccctg ctcgggcaga ccgaacgcga acgcgacgaa ctggccgccg
    23821 actgccagcg ggcgtccgtt gcgcaacgga ccgcgtccgt gcaactggag cagctggtca
    23881 cctaccgtcg cgagtacgag gagcgctggg cccatcagtt ccgcacggac ggccggatgg
    23941 aactggtcaa ctgctacagg ggattcatgg aacgcctgac ccaggcggtc gaacagcagc
    24001 gccgtgtcgc ggttcaggcc ggggccgagc tggaacaggc gcagctcgcg ctgcgagagg
    24061 gcgagatccg cgtggcctcg gttcgcaagc tgatcgagcg gcgcgtcgcc gacgtgcgtg
    24121 tcgcggtcga tcgccgagaa cagaaggcgt ccgacgagtt cgcgtcgcgt gccgcatgga
    24181 tgcagcgctc caacggcctg gcgggcgcgc tgtgagcgcc ctgaattcga atgcgacggc
    24241 gggcctgccg cttctgcaat cgttgaacac ggcgggcgtt tcgacttcgt cgaacccgtc
    24301 cgcagcaagt gccaggtctg ccggcgaggg gcagggtggt ggcccttccg acttctcgcg
    24361 cctgctggtg cgcagccagg cggcagccga ctcgcagcgg accgtcgcgc cagctggcag
    24421 gacgccaccg ccgcaacggc cggcgtcgcc gacaccgaat tcgcgccccg agacgcctgg
    24481 cccgcgtgca gaacgcgacg ccagcgatgc cgatgcgtcg agtggagttg ccgagggcgc
    24541 cgacaccgcg gcctgcgcct gcgcgacggc tgaacagccg tcggagcggg cgggtccgct
    24601 gcgatccagg cgttctgcgc cggctggcaa ggcacccgcc aacgcggcgt ccgcggaggt
    24661 cgcaggcgcc gaagccacca gcacatggga tgcacgcacg cccaaggctg caccggctgc
    24721 accggctgcc gccgagctgg cgtcgcttgc agcctggggc gccttgccga tgcctgctgc
    24781 cgcaccaccg gctgcaaacg ctgattccga aggcgagatc gctgccccgg atgggacagc
    24841 gacgtctgct gcctcatcgg aacccgcctc atcctccccg atcgatgccc gcgctgccga
    24901 gtcgatggac gcgttcgaag ccagtgatcg ggacgcggcg acccgagcca tggcgcagga
    24961 gtcgccgctg gctctggatg cctcgtccct gccggccggc ggggcggcac ctgacgttgc
    25021 gatggtgaac atggcgatgc tctcggccgg cgtgcgtccg gaactcgaag ccgacctggg
    25081 gcagggcgcc gatctcgcca ttccccccgc gtgggccctg ggtgcggccg gctcgtcgac
    25141 cccctcacgt gccgtcgatg cggcggtgcc ctcggcatct gccactgtcg cgactccgct
    25201 cggcgacagc ggcttccacg aagcgctggg catgcaggtg agcctgctcg cgcgggaagg
    25261 catccagaag gccgaattgc ggttgaaccc ggccgacatg gggccggttt cggtccagat
    25321 cacgatgaac ggcgatcagg cccgtgtcga tttcggcgcc gacctggcgc agacgcggca
    25381 ggtcatcgaa gcggggtggg ccgaactggc cgcttcgctg caggaggccg gcttcacgct
    25441 cagcggcggg ggggtctccg aacacgcccg tgaccctcgc ggtgcgccgc aagggatggt
    25501 gccgccgtcc ccggttgcca cggcatcggc tgacggcgat caggagcccg cagcttcgat
    25561 catcgtgccc cggccgaggg ccggagccgc gctggacctc tacgcctgag cgcannnnnn
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccgg gacgcaagct ccaaatcacc
    25741 cctctttcag tcccttatcg aagcatcgac agcagtcgtc gttttgataa tgaatcgagc
    25801 gttggcaagg tgtcggtctg cctgccaccg ctggatcgtg ttcatcacct gaaggaactg
    25861 tcatggccac tgctgccgcc accgcccctg ctgccgaagc tggtgatgcc cccgcgccca
    25921 agaagggcaa gaagaagctg atcatcatca tcgcggcggt gctgctggtc gtcctggccg
    25981 gcggtggtgc ggcggtctac atgatgaagc agaaggccgc agccgccgaa gccgccgctg
    26041 ccgaagccga gggcgaggac ggcgaagcgg cgccggccaa ggacgcgcac gccaagaagg
    26101 atgaacacaa gggcccgccg gtgttcgtgc cgctggatcc attcgtcgtc aacctggccg
    26161 atcgtgattc cgagcgctac gcgcagatcg gcatcacgct gcaggtcgat gacccgaagt
    26221 tcgccgatga actcaagctc tacctgccgg cggtgcgcaa cggcatcctg atggtgctgg
    26281 cccacaagac gtcgaaggaa ctgctcgagc gcagcggcaa ggaaaggctg gccgccgaga
    26341 tcatgcgcga ggccgtgcgc ccgatgggca tcgagatcgc cctcgaggat ccggaagccg
    26401 atgcgcagca gcacgaagcg cctgccgatc cggacgccga ggaagccccc aagccgaaga
    26461 agaaaaagaa gaagaaggcc ccggaagtcc acaacccggt gaccggcgtc aacttctcca
    26521 gcttcatcat ccagtgagcg tgcggccgac atgaatcagc agatcctgtc gcaagacgaa
    26581 gtcgatgcgc tgctgcaggg catcaccggc gagagccaga agctcgaagc cgaggacgtg
    26641 ccgcagggcg gcatccgcaa ctacgacatt tccagccagg aacggatcgt ccgtgggcgc
    26701 atgcccacga tggagatcat cagcgagcgc tttgcacgca acatccgcat cggcctgttc
    26761 aacatgatcc gcaagagccc cgaggtgtcg atcggtggca tcaaggtgca gaagtacagc
    26821 gccttcctgc gcgagatcgt ggtgcccacc aacttcaaca tcgtcagcgt caagccgctg
    26881 cgcggcagcg ggctgatcgt gtgcg
//
LOCUS       DAOORV010000038        26482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2189_length_26482_cov_7.883415, whole genome shotgun sequence.
ACCESSION   DAOORV010000038 DAOORV010000000
VERSION     DAOORV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_26482_cov_7.883415"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKV56_07150"
     CDS             complement(<1..1534)
                     /locus_tag="PKV56_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX67628.1"
                     /translation="MSTEKPSSSATSADTSGLRATLNLPDTPFPMRGDLPKREPGWVR
                     QWEDAGTYQRLRDARCGAPKFVLHDGPPYANGQIHIGHAVNKVLKDMIVKARQLKGMD
                     ASYIPGWDCHGLPIENQIEKTFGRKLPRAEVQAKSRAYATEQIAQQMGDFKRLGVLAD
                     WAHPYRTMDFGNEAGEIRTLKRVMERGYVYRGLKPVYWCFDCGSSLAEFEIEYADKTS
                     QSLDVAFLCAEPDRLASAFGLPSLHKDAYAVIWTTTAWTIPANQALNLNPALEYSLVD
                     TERGLLLLASARVEDSLQRYGLQGTVVGTALGEKLDGVNFRHPLAHVDAGYDRLSPIY
                     LADYATADDGTGLVHAAPAYGVDDFKSCVAHGMAIDEILNPVTGSGVYDASLPLFGGQ
                     HIWKACAVIIDTLRAAGRLLATATFQHSYPHCWRHKTPVIYRAAAQWFVRMDEANDDT
                     AGVFATDPAPQTLRQSALAAIDATTFYPENGRVRLRDMIANRPDWCISRQRNWGVPLP
                     FFL"
     gene            complement(1862..2830)
                     /locus_tag="PKV56_07155"
     CDS             complement(1862..2830)
                     /locus_tag="PKV56_07155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOX67629.1"
                     /translation="MLLFRGFNHPGVAPACALTIGNFDGVHRGHQAMLALLRNEAQHR
                     GLPSCVLTFEPHPRDHFAARSGQPDLAPARISTLRDKLSELERCGVDQAIVVPFNDRF
                     AAQAPQAFIDDVLVRGVRARYVLVGDDFCFGARRAGDYAMLDAAGQAQGFDVARMLSY
                     EVRGLRVSSSAVREALGVGDMARARSLLGRPYSISGHVVHGRKLGRDLGFRTLNLRFP
                     HTRPAAMGIFVVRVHGLTPDGKPLSGVASLGVRPTVENAGQVLLETHCLDWPEALGSE
                     GGYGRCIRVELMHKLHDERHYESIEALRAGIAQDELEARTWLATLA"
     gene            complement(2838..3617)
                     /locus_tag="PKV56_07160"
     CDS             complement(2838..3617)
                     /locus_tag="PKV56_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX67630.1"
                     /translation="MKLSQVLYTQGFGSRRECEGLIVSGFVSVGQVVCDDPFEEFDPE
                     GLRFTVRGESWPFREKALIVLNKPAGHECSQKPKHHPSVYSLLPAPLRRRDVQAVGRL
                     DEDTTGLLLMTDDGGLIHRWTSPKHHVPKVYEIGCKHPVTAEQVRQLLAGVTLIDDPV
                     PVRAAEAEATGAFAGPQAPGLGELHGLRMTLTEGKYHQVKRMVAAVGNRVEALHRSRY
                     GALVLPADLAPGQWRWLAGPSDVAPVDGAASRARPKSAPPQ"
     gene            complement(3614..4291)
                     /locus_tag="PKV56_07165"
     CDS             complement(3614..4291)
                     /locus_tag="PKV56_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOX67631.1"
                     /translation="MTALARWVRFSRLAGVLAAAGGGLLCAGPVGAQSVSFSGHMGDK
                     ALLVIDGTPRTLATGTSQSGVRLVSVNPGAAVIEVGGKRQTLTLGGSPVSLGGANSEG
                     GGEQIVLTAGLGGHFITGGSINGKPVEFMVDTGATTVAMSAAEADRIGLKYKDGPRMT
                     GNTANGQVTGYRASLNSVRIGDVQVYNVEAAILPLPMPHILLGNSFLTRFQMRRENDR
                     MTLDKRP"
     gene            complement(4296..4784)
                     /locus_tag="PKV56_07170"
     CDS             complement(4296..4784)
                     /locus_tag="PKV56_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOX67632.1"
                     /translation="MPSFDTVLEPNLVEVRNAVDQASKEIGTRFDFKGSSSKVELKDK
                     ELTVWADSDFQIGQVMDIVLAKLAKRSVDVRYLDRSAKIEKIGGDKVKQLIVIKNGID
                     GETAKKLQTAVKQSKLKVQAAIQGDTVRVTGAKRDDLQAAMALIRKDLPDSPLSFNNF
                     RD"
     gene            4817..5851
                     /gene="murB"
                     /locus_tag="PKV56_07175"
     CDS             4817..5851
                     /gene="murB"
                     /locus_tag="PKV56_07175"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOX67633.1"
                     /translation="MDENSAMHIESSVSLRAYNSFGLPAVAHTLVRIASDADVRRVVD
                     HPTLGRAPKFILGGGSNIILTRDMPQVVLKVEVTGCRLVEERADAWVVEAGAGESWHD
                     LVMWCLAQGYPGLENLALIPGTVGASPVQNIGAYGVELQDRFDSLDAVDLRTGLTLTL
                     SPQQCAFGYRDSVFKHSLAGCAVITRVRFRLPRPWKPVLGYLELERKVAETGIAQPDA
                     RQIADWVIAIRRAKLPDPARIGNAGSFFKNPVVTPEQCRDIIGRDPEIVHYPMADGSV
                     KLAAGWMIDACGWKGKTVGQAGVYEKQALVLVNRGDAIGSEVMTLAKAIQESVYGRFG
                     IRLETEPVVV"
     gene            5863..6681
                     /locus_tag="PKV56_07180"
     CDS             5863..6681
                     /locus_tag="PKV56_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67634.1"
                     /translation="MQRRLLLHSRRLVAGLTALAIVALAVGVSGWLPGRSLWLMLFAL
                     LVTVIIATTLWSNWQRDLAIREAPLPQFLKRKLRETYPQLIGKDCDLVERGLRQFFLA
                     CKRSDRRFVAMPSRAVDVMWHEFILHTRAYQDWCDLTLGRFLHHTPAETLGAKASGND
                     GLRRAWTWACRDEAIDPRRPSRLPLLFALDAKLGIPNGFSYVPDCGDIDHQNRQGGGN
                     GGVYCGTSFSDGSYSGDADGLGGADSSPGDGGSDAGGGDGGGGGGCGGGGGGGD"
     gene            complement(6708..8540)
                     /locus_tag="PKV56_07185"
     CDS             complement(6708..8540)
                     /locus_tag="PKV56_07185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOX67635.1"
                     /translation="MDTRSSTIRPRIESVREELRRHGLAAVLVPSSDPHLSEYLPERW
                     QGRQWLSGFTGSMGTLVVTTETAVLFADSRYWVQAEAELAGSGVELFRIPTGAATHPI
                     DWLASHVASGQTVGVDGDALGLATAHLLQRTLKAAGIALRTDFDVLAAIWPDRPGLPG
                     APVYEHVAPQATETRSKRLARVREAMVRAGASHHFISTVDDIAWLFNLRGSDVLYNPV
                     FLAHALIDASQATLYVAAGKISPALRAVLATDGVRIDSYDRAGPSLGALPAGSSLLID
                     PRRVTLGLRQRVRDSGPEPIRVIEAINPSTLLKSRKNSTEAAHIREAMAQDGAVMCGF
                     YAWFEQALADPAQAGRLTELTVDERLSAARSERPGFVGLSFPTIAGFNANGAMPHYRA
                     TAESHAVIEGNGLLLIDSGGQYLGGTTDITRVWPIGTPSAEQKRDYTLVLKGTMALSR
                     TRFPRGTLSPMLDSIARAPLWQHGLDYGHGTGHGVGYFLNVHEGPQSISKTIPESTMA
                     MEPGMVTSIEPGLYRPGRWGIRIENLVMNVPAESPEGDTFGEFLEFETLTLCPIDTRC
                     LDRSLLDAGEIAWLNRYHAMVFERLAPRVSGDALHWLTERTRTL"
     gene            complement(8596..9954)
                     /locus_tag="PKV56_07190"
     CDS             complement(8596..9954)
                     /locus_tag="PKV56_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOX67636.1"
                     /translation="MPNSLRLTLLSVRDLLATVGPFALLAVALLVPAYYVLDPTPPKK
                     VVLATGVDQGAYAEFGQRYKTLLKTYGIEVELRSTQGAAENLALLRAPASGVDLAFVQ
                     GGADDIAAPAGSDDEPAPLVSLGSLFFEPVWLFYREDSAQRLLKSGTLSGLAQLAGWK
                     LNIGAPGSGVPNLMKKLLEANHVDPKQITLLEQSQTPAVMALLGAESDALVFASAPES
                     LMVQMLLQTPGIKLFDFAQAEAYSRRFAFMSPVVLPRGVVDLAGDRPPQDVHLVAPTA
                     TLVAREGTHPALIQLFVQAAQQVHGGAGWFQRKGEFPTALNTERPLAKEAQRFYQSGV
                     PLLQRYLPFWFANLIDRMWPVLVTIVAVLLPLSRMLPPLYEFRIRSRVFRWYEQLREL
                     EDTIGAKPSAQWLEEIAALESRVERITVPLAYTDELYALRSHIALVRSRLPTAGHPTP
                     GP"
     gene            complement(10035..10997)
                     /gene="argF"
                     /locus_tag="PKV56_07195"
     CDS             complement(10035..10997)
                     /gene="argF"
                     /locus_tag="PKV56_07195"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOX67637.1"
                     /translation="MKPGKALIRHYLQFKDLRAEEYAYLLARTTVIKKRFKNYEKYTP
                     LVDRTLAMIFEKASTRTRVSFEAGMYQMGGSVVNLTTGDSQLGRSEPIEDSARVISRM
                     VDLVMIRTYEQTKIEAFAAHSRVPVINGLTNEYHPCQILADVFTYIEHRGGAASTGEA
                     LACLKGKVVAWVGDGNNMANTWLQAADILGFTLHVSTPSGYEIDAKVAGVTNQSCLKV
                     FDNPMDACRGAHLVTTDVWTSMGYEAENEARKKAFAHWRVDRDMMAVAQPDALFMHCL
                     PAHRGEEVDADVIDGPQSVVWDEAENRMHVQKALMEYLLLGRIG"
     gene            complement(11014..12225)
                     /locus_tag="PKV56_07200"
     CDS             complement(11014..12225)
                     /locus_tag="PKV56_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOX67638.1"
                     /translation="MNAPETAALDPEPHVMHTYGRLPMAMSHGRGCWVWDTDGKKYLD
                     GLGGIAVNTLGHAHPKLVPALQEQIGRLIHTSNYYASPLQESLAATLCELSGLERVFF
                     CNSGLEANEAALKIARKFGHDKGIDRPEVIVYEKAFHGRSIATLSATGNPKVQEGFGP
                     LVEGFVRVPLNDVAALEEVANTRPNVVAVFLEVIQGEGGVRPATLEYLRSVRRLCDEN
                     DWLLMLDEVQCGIGRTGKWFAHQWAGIVPDVMPLAKGLGSGVPVGAVVCGPRAADVLQ
                     PGNHGTTFGGNPLAMRAGIETLRIMREDHLLENVATVGAALRASLTRELSGVAGVVEI
                     RGQGLMLGIELDRPCGDLLLKAAQAGLLLSVTADRVVRLVPPLILNLAEAEQIAMTLC
                     PLIKDFLRSPD"
     gene            12487..12819
                     /locus_tag="PKV56_07205"
     CDS             12487..12819
                     /locus_tag="PKV56_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HOX67639.1"
                     /translation="MSSSPPREVFIQGITLSGQTFRPSDWAERLAGAMSSFRPEGSTG
                     DRSSFIGYSPYCVPRVINGIKCVIVDERLRDAEPMAWDFVMAFARDNELQVADACLLD
                     PPKSKPEQ"
     gene            complement(12893..13195)
                     /gene="rpsT"
                     /locus_tag="PKV56_07210"
     CDS             complement(12893..13195)
                     /gene="rpsT"
                     /locus_tag="PKV56_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOX67640.1"
                     /translation="MATSSKAKKKTVRIASGLKRVRQDVKLNAANTALRSKFRTVIKN
                     VQKAVIAGDKAKAADLFKSAEKVIDSVADKGLFHKNKAARHKSRLSAKIKALPATA"
     gene            13362..14918
                     /gene="murJ"
                     /locus_tag="PKV56_07215"
     CDS             13362..14918
                     /gene="murJ"
                     /locus_tag="PKV56_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOX67641.1"
                     /translation="MNLLRAASTVSLLTLASRITGLGRELLIATSFGASASTDAFNVA
                     FRIPNLLRRLFAEGAFSQAFVPILAATRASEGEAATHQLVSAVATVLLWALALVSVIG
                     VAMAPVLVWLMASGLQEFDQAVVMTRVMFPYIAFMSLVALAAGVLQTWKRFAVPAATP
                     VLLNLSVMLAAWLLGPWFQRHGIEPVYALAAGVMLGGVLQLAVQWPALRAIGGLPRIG
                     LTWSALRVAMQHPGVGRILRQMAPALLGVSVAQLSLLINTQIASHVAVGAVSWLTYAD
                     RLMEFPTALLGVALGVVLLPQLSAAQASGDEAAYSSHLDWGLRLVLLLALPCAVALLV
                     FPKPMVSVLYHYGQFSERDVQQTVRALMGYGVGLIGLIGVKVLAPGFYARQDIRTPVK
                     IAIVVLILTQLMNLLFVPRLGHAGLALSIGLGALINATWLLIGLRRQGTYVPQAGWLG
                     FSLRVVCASSLLGLALGAAAVGIDWVGLSSQRLLRVGLLAACLLGVGLVYFVALRLMG
                     IRLKDFARRG"
     gene            14960..15841
                     /locus_tag="PKV56_07220"
     CDS             14960..15841
                     /locus_tag="PKV56_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX67642.1"
                     /translation="MPGPYQYATPTALEYFSSLVAEDASLSLLEAAASIAQDEHPRLD
                     VQAVLAEVDELAVKLKRRIPADVVPLQRLRWLNRYFFHELGFGGNVNNYYDASNSYLD
                     DVLRTRRGIPISLAVIYLEVATQLGLRAQGISFPGHFLVKLSLLSGSHNGEVIIDPFT
                     GKSLSREELDEMLTPYKRSQGLVGEFDAPLGLFLQAAAPREILARMLRNLKEIHSTAE
                     DWPRLLHVLNRLVVLLPHAHEERRDRALVWAELGQADRAAEDFEAYLHHRPDADDSTA
                     MAERLERMRRGEQPRLH"
     gene            15900..16592
                     /gene="hda"
                     /locus_tag="PKV56_07225"
     CDS             15900..16592
                     /gene="hda"
                     /locus_tag="PKV56_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HOX67643.1"
                     /translation="MKQIPLPIPSGSTPTFDSFIPGSNAMVLAHLRDLGTQPAPLYLW
                     GPQGSGKTHLLRALAHQTQAQGGRVGWFDPSEPAPWLHEESWSLIVLDGCDAFDAAQQ
                     HAAFALFVEATTHGTPLVAAGRLPPVDLPLRDDLRSRLGWGHVMAVHPLSEAESRGAL
                     RREADGRGLFLSDEVMDHLLHRHARDMKHLMTQLDRLDEFAMVHKRAITVPLLRQMLS
                     EASGDGLTGTLL"
     gene            16589..17257
                     /locus_tag="PKV56_07230"
     CDS             16589..17257
                     /locus_tag="PKV56_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOX67644.1"
                     /translation="MNLCLFDLDDTLLPLDSDHAWGEFVISLGWVDEDSFRRTNDAFF
                     AQYQAGQLDVHGYIEFATRPLRERPADELAAAHSLFMQRVIEPQLRDSARDLVRHHQA
                     TGDRVVLVTATNEFVTAPIAAAFGIESLIAVRLERDARGSISGRIEGTPSYREGKVRR
                     VEEWLGEQGLSWADCERITVYSDSLNDLPLLEKATDPVATNPSPALLSVANERGWRTL
                     NLFT"
     gene            17254..18789
                     /gene="pcnB"
                     /locus_tag="PKV56_07235"
     CDS             17254..18789
                     /gene="pcnB"
                     /locus_tag="PKV56_07235"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOX67645.1"
                     /translation="MIKKFLDKLLGKPAKKTDGASSLGKRAEVSKSEHQIDPGLVDER
                     ASKVVQTLVDAGYEAYIVGGAVRDLLLGLRPKDFDVATNATPEQVKSLFRRAFIIGRR
                     FRIVHVVFGRGRQDRGLSEVIEVSTFRALMDPAAAEQVTGSEKTLKAEGAGRHAGPSH
                     VVDAEGRVLRDNVWGPQIEDAARRDFTVNAMYYDPVREVVVDYHGGIKDAKKKLLRMI
                     GDPETRYREDPVRIIRAVRFAAKLGFEIEPKTRAPIQSMSTLLENVPPSRLFDEMIKL
                     LQTGHSLASIAELRKQGLHQGTFPVLDTLLDESRGSAAHRKFVGLAMADTDRRVSEGK
                     PVAPSFMLACMFWHDVQSRWNQLKHAGEPAFPALQQAVEAIFEARIGDISGRGKLGGD
                     MREIWLMQPRFERRVGSGPFTLVEQPRFRAGFDFLRLRADAGEAPDELARWWEDFSLA
                     DEDERRRLMDDAKVHDGKKRQPASAGEAPPVADPAKKRRRRRRKPTEAGGSAPADGEN
                     EAV"
     gene            18786..19268
                     /gene="folK"
                     /locus_tag="PKV56_07240"
     CDS             18786..19268
                     /gene="folK"
                     /locus_tag="PKV56_07240"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOX67646.1"
                     /translation="MTEARAYVGLGANLGDARAVLAAAYAEVQRLPQTSRHRCSPWFR
                     TAPIDSSGPDYINAVACFDTTLTPQDLLEALQRIENAHGRERPYRNAPRTLDLDVLLF
                     GEQTIATPTLSVPHPRMHERAFVLQPLASLAPEAVIPGRGPLSALLALVSEQAIERLD
                     "
     gene            19619..19870
                     /gene="rpsP"
                     /locus_tag="PKV56_07245"
     CDS             19619..19870
                     /gene="rpsP"
                     /locus_tag="PKV56_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOX67647.1"
                     /translation="MVVIRLSRGGAKKRPFFNIVVADSRVRRDGRFIERVGFYNPVAS
                     GAEQPLRVAFDRIAYWTGTGAQLSPTVARLVEQAKAATV"
     gene            19923..20537
                     /gene="rimM"
                     /locus_tag="PKV56_07250"
     CDS             19923..20537
                     /gene="rimM"
                     /locus_tag="PKV56_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560115.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOX67648.1"
                     /translation="MTASQTGMAVQSDSGFDAVAWPDDAIEVGLIVDAWGVKGWFKVK
                     AYSSDPQALFSSRRWLLKAPEVPGPRTLGHTLQLPPLLKITAAKEHGDVVVAQAQDVV
                     DRAGAEALRGARVFVSRGSFPSAGPDEYYWVDLVGLSVFNRQGEFLGDVVGLIDTGPH
                     SVLRIAPTGVTDEAAQRLIPFVAAFVDSVSLTDRRITVDWGLDY"
     gene            20557..21330
                     /gene="trmD"
                     /locus_tag="PKV56_07255"
     CDS             20557..21330
                     /gene="trmD"
                     /locus_tag="PKV56_07255"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOX67649.1"
                     /translation="MRFDVITLFPELFKPHLIHGITRRAYDSGQVDVRLWPLRDFADG
                     QYRRVDDRPYGGGPGMVLMAEPLERALSAVRAARASDVSELTPVPVIHFTPTGRRLDQ
                     AVVAEMAAGPGAVLLCGRYEGIDQRFLDRHVDIELSLGDFVLSGGELPALALLDAVAR
                     LQDGVLGDAQSHQQDSFSEGLLEGPHFSRPEVLSTDGGLLPIPPVLLSGHHGQIARWR
                     REQSLALTARRRPDLISAARAAKRLTVDDERFLAGLDVY"
     gene            21452..21805
                     /gene="rplS"
                     /locus_tag="PKV56_07260"
     CDS             21452..21805
                     /gene="rplS"
                     /locus_tag="PKV56_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOX67650.1"
                     /translation="MDLIQTLEQEEIARLNKTIPAFAPGDTVIVNVNVVEGTRKRVQA
                     YEGVVIAKRNRGLNSSFIARKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYL
                     RQRSGKSARIKEKLA"
     gene            21967..22653
                     /locus_tag="PKV56_07265"
     CDS             21967..22653
                     /locus_tag="PKV56_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOX67651.1"
                     /translation="MPPTFDPQQLPILAIDDHLPAVPSERLTATGVRERFARSTVWQP
                     ELVVERVLRQRALVHASVLVPLVVRGDELTVLLTQRADHLSDHPGQISFPGGRAEVGD
                     LTAVATALREAEEEIGLAAKHVQVLGTLPTYTTVTGFIVTPVVGLVELGFELRVDPVE
                     VADVFEVPLTFLMSPGHHRRHAVGEGDDVREFFSMPWQASVDGGPPQCRFIWGATAAM
                     LRNFYRFLSA"
     gene            22688..23677
                     /locus_tag="PKV56_07270"
     CDS             22688..23677
                     /locus_tag="PKV56_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HOX67652.1"
                     /translation="MSLFSVLFALLLEQLKPMQRGTAIHAALTSWLRWAGRNFDAGRD
                     RHAWVVWGITVVLPALFAFAVHAGLAHYSLLLGLIWNVAVLYLTLGFRQFSHYFTDIR
                     DALDREDEPEARRLLTEWRHLDVSELPRTELLRHVIEHSLLAAHRHVFGVFFWFVVLS
                     AIGFGPAGAVLYRTAEFASRYWAYRSPTVGEPTNERLMALSHRMFELMDHFPARLTAF
                     GFAVVGNFEEAINCWRRDAELWRHPNEGVILAAAAGAVGVQLGGTAAPGVTPDRSKTF
                     VRGVGVDDLAADGSTTGMAPVQGHLRSIVGLVWRSVVLWMMLLALLTLANLVG"
     gene            complement(23690..24607)
                     /gene="rsgA"
                     /locus_tag="PKV56_07275"
     CDS             complement(23690..24607)
                     /gene="rsgA"
                     /locus_tag="PKV56_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOX67653.1"
                     /translation="MSKAKVGDAPTTSGLVVSGFGRHYIVESASGERTICHPRGKKSL
                     CVVGDRVRWQITGDEGVIEELEPRRNLLFRQDEWKTKAFAANLDQVLVMVASEPVFSE
                     SQLARALIAAESAGIAASILLNKADLPQIVAARDRLKPYVEMGYRVLEVALKAQPEAS
                     RAILNPLLAGRASLVLGPSGTGKSTLINLLAPDARAQVGEVSQALNSGRHTTTSTQWY
                     WLDAVRSTGLIDSPGFQEFGLKQVDPQKLPALMPDLRDASTRCRFYNCTHRQEPGCGV
                     REALAQGEISESRYRIYGEIRQELEGTRW"
     gene            complement(24621..25925)
                     /locus_tag="PKV56_07280"
     CDS             complement(24621..25925)
                     /locus_tag="PKV56_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX67654.1"
                     /translation="MPNYSVFPTSPALVLVLLFSAALVFSLLIRFWLATRQMRHVAIH
                     SDAVPAAFAGTVSLEAHRKAARYTLTKGSFGLWTMAFSAVVLVGWTLLGGLGLLNAAV
                     RDTVQPLFGNMAYQVALIGTFVAIGALLDLPFEIYQTFRIEQAFGFNRMTWKLYVIDA
                     FKGLLLGALIGLPLAAGMLWIMGSTGQLWWLWAWGAWMAFNLLALVLYPTLIAPLFNT
                     FKPLADASLEARVHALMAKCGFTAKGFFVMDGSRRSAHANAYFTGFGASKRVVFFDTL
                     LDKLSPSEVEAVLAHELGHFKHKHVLKRIVSMFAISLAGFAALGWLSSRIWFYTAFGV
                     QPSMSAPNDALALMLFMLIVPVFGFFVSPLLAGLSRRHEFEADAYACSKSDGRDLSAA
                     LLKLYEDNASTLTPDPLYARFYYSHPPAGERLAAMRALAPTA"
     gene            25998..>26482
                     /gene="orn"
                     /locus_tag="PKV56_07285"
     CDS             25998..>26482
                     /gene="orn"
                     /locus_tag="PKV56_07285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOX67655.1"
                     /translation="MTETLGKSDQNLIWIDLEMTGLFPDTDRIIEIAVIVTDSQLKVR
                     VEGPTIAVHQSDAALDAMDAWNKGTHGRSGLIDRVKASSVDEEAAEAQIIAFLQQYVP
                     KGKSPMCGNSICQDRRFLARTMPTLEAFFHYRNLDVSTLKELAKRWKPGILDGFKKAQ
                     A"
BASE COUNT         4270 a         8506 c         8858 g         4848 t
ORIGIN      
        1 gcaggaagaa cggcagcggc acgccccagt tgcgctggcg gctgatgcac cagtcggggc
       61 ggttggcgat catgtcgcgc agccggaccc ggccgttctc gggatagaag gtcgtcgcgt
      121 cgatcgcggc cagcgcgctc tggcgcagtg tttgtggcgc ggggtcggtg gcgaacacgc
      181 cggcggtgtc gtcgttcgct tcgtccatgc gcacgaacca ctgagccgcg gcgcggtaga
      241 tcaccggggt cttgtggcgc cagcagtgcg ggtaactgtg ctggaaagtc gcggtggcca
      301 gcagtcggcc ggcagcgcgc agcgtgtcga tgatcaccgc gcaggccttc cagatgtgct
      361 ggccgccgaa cagtggcagg ctggcgtcgt agacaccgct gccggtcacc gggttgagga
      421 tttcgtcgat cgccatgccg tgcgcgacgc acgacttgaa gtcgtcgacg ccgtacgccg
      481 gcgccgcatg cacgagaccg gttccgtcgt cggcggtggc gtagtcggcg aggtagatcg
      541 ggctcaggcg gtcgtagccc gcgtcgacat gggccagcgg atggcggaaa ttgacaccgt
      601 cgagcttctc gccgagcgcg gttccgacca cagtgccttg caggccgtag cgctgcaggc
      661 tgtcctcgac ccgcgccgat gcgagcagca gcaggccgcg ttcggtgtcg accaggctgt
      721 actccagtgc cgggttcagg ttcagcgcct ggttggccgg gatcgtccac gcggtcgtgg
      781 tccagatgac agcgtaggcg tccttgtgca gcgacggcag tccgaacgcg ctggccaggc
      841 gatccggctc ggcgcacagg aaggccacat ccagcgactg cgaggtcttg tccgcgtact
      901 cgatctcgaa ctcggccagc gacgaaccgc agtcgaagca ccagtagacc ggtttcagac
      961 cgcggtagac gtagccgcgc tccatcacgc gcttgagcgt gcggatctcg ccggcctcgt
     1021 tgccgaagtc catcgttcga tacggatgcg cccagtccgc cagcacgccc aggcgcttga
     1081 agtcgcccat ctgctgcgcg atctgctcgg tcgcgtaggc gcggctcttg gcctgcacct
     1141 cggcgcgtgg cagcttgcgg ccgaaggtct tctcgatctg gttctcgatc ggcagcccgt
     1201 gacagtccca gccggggatg tagctggcgt ccatgccctt gagctggcgg gccttgacga
     1261 tcatgtcctt gaggaccttg ttcaccgcgt gcccgatgtg gatctggccg ttcgcgtacg
     1321 gcgggccgtc atgcagcacg aacttcggcg cgccgcagcg ggcatcgcgc aggcgttggt
     1381 aggtgcctgc atcctcccat tgcctgaccc atcccggctc gcgcttgggc aggtcgcccc
     1441 gcatcgggaa cggcgtgtcc ggcagattca gcgtggcgcg caggccggag gtgtcggcgc
     1501 tggtcgcact ggaggaaggc ttttcggtgg acatgcttcg gtctcgacag gtgacgtctc
     1561 gtgcaaggcg agtggtaagg ggcgggatcg gggtggggcg agcctcgggt cgatcaagag
     1621 gatcgctccc gacgcaagaa gatgccgcac ccgtgactgc aacggcgcag gctgccgcgc
     1681 gcccggcgcg gcttcgcacc cgtcaaattc ggtcgcgcgt cgtctgccgg tgcgttgcac
     1741 cgtggccgtc ggggcgacgt gtgagggtga tcgagatcat gctcatcggc ccgattctac
     1801 cggggcaggt ccgggcagca gagaccgcgg cgggccgcgc gcgggtgtcc atgcggcctg
     1861 ctcaggccag cgtggccagc caagtccggg cctcgagctc gtcctgcgcg atgccggcgc
     1921 gcagcgcctc gatcgattcg taatgccgct cgtcgtgcag cttgtgcatc agctcgacgc
     1981 ggatgcagcg gccgtagccg ccctcgctgc cgagggcttc gggccagtcc aggcagtggg
     2041 tctcgagcag cacctgtccg gcgttctcga cggtcgggcg cacgccgagg ctggcgacgc
     2101 cgctcagcgg cttcccatcg ggtgtcaagc cgtgcaccct gaccacgaag atgcccatcg
     2161 ctgccggccg cgtgtgcgga aagcgcaggt tgagggtgcg gaagccgaga tcgcgcccga
     2221 gcttgcgccc gtgaaccacg tggccgctga tgctgtacgg acgccccagc agcgatctcg
     2281 cccgggccat gtcaccgact cccagggcct cccgcacggc cgaactcgac acgcgcagac
     2341 ctcgcacctc gtagctcagc atgcgggcca cgtcgaagcc ctgagcctgg cctgccgcat
     2401 ccagcatcgc gtagtcgccg gcccgacggg cgccgaagca gaagtcgtcg ccgaccagca
     2461 catagcgtgc acgcacgcca cgcaccagca catcgtcgat gaatgcctgc ggtgcctgcg
     2521 cggcaaagcg atcgttgaat ggcacgacga tggcctgatc gaccccgcaa cgttccagtt
     2581 cgctgagctt gtcgcgcagg gtcgagatcc gggccggcgc gagatcgggt tggccgctgc
     2641 gcgcggcgaa gtggtcgcgc ggatggggtt cgaaggtcag cacgcaactc ggcaggccac
     2701 gatgctgggc ctcgttgcgc agcagagcca gcatcgcctg gtgcccgcgg tgcacgccgt
     2761 cgaagttgcc gatcgtcagg gcgcaagccg gtgcgacacc gggatggttg aaccctctga
     2821 aaagcagcat gccgcgatta ttgcggcggt gctgacttag gccttgctcg gctggctgcg
     2881 ccgtcgaccg gtgcgacatc cgagggcccg gccagccatc gccactggcc cggtgccaga
     2941 tcggccggaa gcacaagcgc gccgtagcgg ctgcggtgca gtgcttcgac gcggttgccg
     3001 acggccgcca ccatgcgctt gacctggtgg tacttgcctt cggtcagcgt catgcgcaga
     3061 ccgtgcagct cgcccagtcc cggcgcctgc ggaccggcga atgcgcccgt ggcctcggcc
     3121 tctgcggcgc gcaccggcac cggatcgtcg atcagggtca cgccggccag cagttgccgg
     3181 acctgctccg cggtcaccgg gtgcttgcaa ccgatctcgt agaccttggg gacgtggtgc
     3241 ttgggcgagg tccagcggtg gatcaacccg ccgtcatcgg tcatcagcag caggccagtg
     3301 gtgtcctcgt cgagccgccc caccgcttgc acgtcgcggc ggcgcagcgg tgcgggcagc
     3361 aggctgtaga cgctcgggtg gtgcttgggc ttctgcgagc actcgtggcc cgcgggcttg
     3421 ttcaggacga tcagcgcctt ctctcgaaac ggccacgatt cgccgcgaac cgtgaaccga
     3481 agcccttcgg ggtcgaattc ctcgaacggg tcgtcgcaaa ccacctggcc gaccgaaacg
     3541 aagccggaga cgatcaggcc ctcgcactcg cggcgcgagc cgaaaccctg cgtgtacagc
     3601 acctgcgaca gcttcaaggg cgcttgtcca gcgtcatgcg gtcgttctcg cgtctcatct
     3661 ggaagcgggt caggaagctg ttgcccagca ggatgtgcgg catcggaagc ggcagtatgg
     3721 cggcttcgac gttgtagacc tgcacgtcgc cgatgcgcac gctgttcagc gaagcgcggt
     3781 agccggtgac ctggccgttg gcggtgttgc cggtcatgcg gggcccgtcc ttgtacttga
     3841 ggccgatgcg atcggcctcg gcggcgctca tcgcgacggt ggtggcgccg gtgtcgacca
     3901 tgaactcgac cggcttgccg ttgatgctgc cgccggtgat gaagtgcccg cccaggccgg
     3961 cggtcagaac gatctgttca ccgccgcctt cgctgttcgc gccgcccagg ctcaccggag
     4021 agccgccgag tgtcagcgtc tgccgcttgc cgccgacctc gatcaccgct gcgccggggt
     4081 tcacgctgac cagccttacg ccggattgcg aggtacccgt ggccagggtt cgcggcgtgc
     4141 catcgatcac cagcagcgcc ttgtcgccca tgtgcccgct gaacgaaacg ctctgcgcgc
     4201 caaccggacc cgcgcacagc aggccgcccc cggccgcggc aagcaccccg gccagccgcg
     4261 agaaccgcac ccaccgcgca agagccgtca ctcgctcagt cccggaagtt gttgaacgac
     4321 agcggcgaat cgggcaggtc cttgcggatc agcgccatcg ctgcctgcag gtcgtcgcgc
     4381 ttggcgccgg tgacgcgcac cgtgtcgccc tggatcgcgg cctgcacctt cagcttgctc
     4441 tgcttgacgg cggtctgcag cttcttggcc gtctcgccgt cgatgccgtt cttgatgacg
     4501 atcagctgct tgaccttgtc gccgccgatc ttctcgatct tggcgctgcg atcgaggtat
     4561 cgcacgtcga cgctgcgctt ggccagcttg gcgagcacga tgtccatcac ctgcccgatc
     4621 tggaaatcgc tgtcggccca gaccgtgagc tccttgtcct tgagttcgac cttggaggac
     4681 gaacccttga aatcgaagcg cgtgccgatt tccttcgagg cctggtcgac ggcgttgcgc
     4741 acctcgacga ggttgggttc gagcacggtg tcaaagctgg gcatggtggt cgcgctttcg
     4801 gatcaacagg tcttcgatgg acgaaaattc tgccatgcac atcgagtcga gcgtcagtct
     4861 gcgtgcctac aacagcttcg gtttaccggc cgtagcgcac accttggtgc gcattgcgag
     4921 tgacgccgat gtgcggcgcg tggtcgatca tcccacgctc gggcgggcgc ctaagttcat
     4981 cctgggcggg ggcagcaaca tcatcctgac ccgagacatg ccacaggtgg tgctgaaggt
     5041 cgaggtgacg ggctgccggc tggtcgagga gcgcgccgac gcatgggtcg tcgaggccgg
     5101 tgccggcgag tcctggcacg acctggtgat gtggtgcctg gctcagggct acccgggcct
     5161 ggagaacctg gcgctgatcc ccgggacggt cggcgcgtct ccggtgcaga acatcggcgc
     5221 ctacggggtc gaactgcagg atcgtttcga ctcgctcgac gcggtcgacc tgcgcaccgg
     5281 actcacgctg accttgagcc cgcagcagtg cgccttcgga tatcgcgaca gcgtgttcaa
     5341 gcactcgctg gccgggtgcg cagtgatcac ccgggtgcgg ttccggctgc ccaggccgtg
     5401 gaagccggtg ctcggctacc tcgaactcga acgcaaggtg gccgaaaccg ggattgcgca
     5461 gcccgatgcc cggcagatcg ccgactgggt catcgccatc cgccgcgcca agctgcccga
     5521 cccggcccgc atcggcaacg ccgggagctt cttcaagaac ccggtggtca cgcccgagca
     5581 gtgccgcgac atcatcggtc gcgatccgga aatcgtgcac tacccgatgg ccgacggcag
     5641 cgtgaagctg gccgccggct ggatgatcga tgcctgcggc tggaagggaa agacggtcgg
     5701 ccaggccggc gtgtacgaga agcaggcgct ggttctggtc aaccgcgggg atgccatcgg
     5761 atccgaggtg atgacgctcg ccaaggcaat ccaggaaagc gtctacggac ggttcggcat
     5821 ccggctcgag accgaaccgg tggtcgtttg agatccctcg ccatgcagcg acgcttgctg
     5881 ctgcattcgc ggcgtctggt tgccggcctc accgcgctcg cgatcgtggc cctcgcggtt
     5941 ggagtttctg gctggttgcc cggacgatcg ctgtggctga tgttgttcgc cttgctggtc
     6001 acagtgatca tcgcgacgac gctctggtcg aactggcagc gtgatctcgc cattcgcgag
     6061 gcgccgctgc ctcagttcct caagcgcaag ctgcgcgaga cctacccgca actgatcggc
     6121 aaggactgcg acctggtcga acgcggactg cgccagttct tcctggcctg caagcgcagc
     6181 gaccgtcggt tcgtcgcgat gccgtcgcgg gcagtcgatg tgatgtggca tgaattcatc
     6241 ctgcacacgc gggcgtatca agactggtgc gacctgacgc tgggtcgctt cctgcatcac
     6301 acaccggccg agacgctcgg cgcgaaagcc tccggcaacg acggcctgcg ccgtgcctgg
     6361 acctgggcct gccgcgacga ggccatcgat ccgcgcaggc cttcgcggct cccgctgctt
     6421 tttgcgctcg acgccaagct cggcatacct aacggcttca gctacgtgcc cgactgcggc
     6481 gacatcgatc accagaatcg ccagggcggt ggcaacggag gggtctactg tggaacgtcc
     6541 ttttcggatg gctcctacag cggagacgct gacggactgg gcggggccga cagctccccc
     6601 ggggacggcg gcagcgacgc aggcggtggt gatggagggg gcgggggagg gtgcggcggt
     6661 ggcggtggcg gaggggactg atccgttgct tgacggatgc tccggcgcta cagcgttcgc
     6721 gtgcgttcgg tcagccagtg cagtgcatcc cccgacaccc gcggcgccag ccgctcgaac
     6781 accatcgcgt ggtaacggtt caaccacgcg atctcgccgg catcgagcag cgaacgatca
     6841 aggcagcggg tgtcgatcgg gcacagtgtc agcgtctcga attcgaggaa ctcgccgaac
     6901 gtgtcgccct cgggtgactc ggccggcacg ttcatgacga ggttctcgat gcggatgccc
     6961 caccgcccgg gccggtacag gccgggctcg atgctggtga ccatgcccgg ctccatcgcc
     7021 atggtcgact cggggatggt tttcgagatg ctctgcgggc cttcgtgcac gttcaggaag
     7081 tagccgacac cgtgtccggt gccgtgaccg tagtcgagcc cgtgctgcca caggggcgcg
     7141 cgggcgatcg agtcgagcat cggtgacagt gttccgcgcg gaaagcgcgt tcggctcagc
     7201 gccatcgtgc ccttgagcac cagcgtgtag tcgcgcttct gttcggcgct cggcgtgccg
     7261 atcggccaga cgcgggtgat gtcggtggtg ccgcccagat attggccgcc cgagtcgatc
     7321 agcagcaggc cgttgccttc gatcaccgca tgcgactccg ccgttgcgcg gtagtgcggc
     7381 atcgccccgt tggcattgaa gccggcgatg gtcggaaagc tcaggccgac gaagcccggg
     7441 cgttcgctgc gtgccgcgct aagtcgttca tcgaccgtca gttcggtcag gcgaccggct
     7501 tgggccggat cggccagcgc ctgctcgaac cacgcataga agccgcacat cacggcgcca
     7561 tcctgtgcca tcgcctcgcg gatgtgtgcg gcctcggtcg agttcttgcg gctcttcagc
     7621 agcgtgctgg ggttgatggc ctcgatcacg cggatcggct cgggcccgct gtcccgcaca
     7681 cgctggcgca gaccgagcgt cacccggcgt ggatcgatca gcaggctgga gccggcaggc
     7741 agcgcaccca gcgacggtcc ggcacggtcg taggaatcga tccgcacgcc atcggtcgcc
     7801 agaactgcgc gcaatgccgg tgagatcttg ccggcggcaa cgtaaagggt ggcctgactc
     7861 gcatcaatca acgcgtgcgc caggaacacc gggttgtaga gcacgtccga cccgcgcagg
     7921 ttgaacagcc aggcgatgtc gtcgaccgtc gagatgaaat ggtgcgatgc accagcacgc
     7981 accatggcct cgcgcacgcg cgccagccgc ttggagcgcg tctcggtggc ctgcggtgcg
     8041 acatgctcgt agaccggtgc gccgggcagg ccgggccgat ccggccagat ggcagccagc
     8101 acgtcgaagt cggtgcgcag tgcgatgccc gcggccttca acgtgcgctg cagcaggtgt
     8161 gcggtggcga ggccgagcgc gtccccgtcg actccgacag tctggcccga agcgacgtgg
     8221 ctggccagcc agtcgatcgg gtgcgtggcg gcaccggtcg gaatcctgaa gagttcgacg
     8281 ccgctgcccg cgagttcggc ctcggcctgc acccagtacc ggctgtcagc gaacagcacc
     8341 gcggtttcag tcgtgaccac cagcgttccc atcgacccgg tgaagcccga aagccactgc
     8401 cgtccctgcc agcgttccgg caggtattcg gacaggtgcg gatcggagga gggcaccagt
     8461 acggcggcaa gcccgtgtcg gcgaagctct tcgcgcacgc tctcgatgcg cgggcggatg
     8521 gttgagctgc gtgtgtccat gccccgaagt gtaggtttgg ggtgcggcca gagcagacgc
     8581 cggcaacgat gcccgctaag gtcccggggt gggatgacct gccgtgggca agcgcgagcg
     8641 caccagtgcg atgtggctgc gcagggcata gagctcgtcg gtgtaggcca gcggcacggt
     8701 gatgcgctcg acgcgtgact ccagcgcggc gatctcttcg agccactgcg ccgacggctt
     8761 cgcaccgatc gtgtcttcca gttcgcgcag ttgctcgtac cagcggaaca cccgcgagcg
     8821 gatgcggaac tcgtacagcg gcggcagcat ccgcgaaagc ggcagcagca ccgcgacgat
     8881 ggtgaccagc accggccaca tgcggtcgat cagattggcg aaccagaacg gcaggtagcg
     8941 ctggagcagt ggcacgccgc tctggtagaa acgctgggct tccttggcga gcggacgttc
     9001 ggtgttgagc gcggtcggga actcgccctt tcgctggaac cagcctgcgc ctccatgaac
     9061 ctgctgagcc gcctgcacga acagctgtat cagtgccgga tgcgtgcctt cgcgtgccac
     9121 cagcgtggcg gtgggggcca ccaggtgcac gtcctgcggt ggccggtcgc cggcgagatc
     9181 gaccacgccg cgcggcagca ccaccgggct catgaaggcg aagcgtcgcg agtaggcttc
     9241 ggcctgcgcg aagtcgaaca gcttgattcc cggtgtctga agcagcatct ggaccatcag
     9301 cgattcgggc gccgaggcaa ataccagggc atccgattcg gcgccgagca gcgccatcac
     9361 cgccggtgtc tgcgactgct ctagcagcgt gatctgcttc gggtccacgt gattggcttc
     9421 gagcagcttc ttcatcaggt tcggtacgcc gctgccgggc gcaccgatgt tgagcttcca
     9481 gccagccagc tgggccaggc ccgacagcgt gccggacttc agcaggcgct gggcggagtc
     9541 ttcccggtag aacagccaca cgggttcgaa gaacaggctg cccagtgaca ccagaggggc
     9601 gggttcgtcg tcggacccgg ctggcgcagc gatgtcgtcc gcgcccccct gcacgaacgc
     9661 caggtcgacg cctgaagccg gggcgcgcaa cagcgcgagg ttctcggccg cgccctgggt
     9721 gctgcgaagt tcgacctcga tgccgtaggt cttcagcagt gtcttgtagc gctggccgaa
     9781 ctctgcgtag gcgccctggt cgacgccggt ggccagaacg accttcttcg gcggtgtcgg
     9841 gtcgagcacg taataggcgg gtacgagcag cgcgaccgcg agcagtgcga acggcccgac
     9901 ggtggcgagc aggtcgcgca cggacagcag ggtgaggcgc agggaattgg gcatggtgtc
     9961 agcccgcgcg aagccgcgcg gttcgcgtgc aggtcggtga tcagggcgag ctttatttca
    10021 cccgcgccgg cgcgtcagcc gattcggccg agcagcaggt attccatcag ggccttctgc
    10081 acatgcatgc ggttctcggc ctcgtcccac acgacacttt gcgggccgtc gatcacgtcg
    10141 gcatcgacct cctctccgcg atgcgcgggc aggcagtgca tgaacagcgc atcgggctgg
    10201 gcgactgcca tcatgtcccg gtcgacgcgc caatgggcga atgccttctt gcgcgcctcg
    10261 ttctcggctt cgtagcccat gctggtccac acgtcggtgg tcaccaggtg cgcgccgcgg
    10321 caggcgtcca tcgggttgtc gaacaccttc aggcacgact ggttggtcac gcccgccacc
    10381 ttcgcgtcga tctcgtagcc gctcggtgtg ctgacgtgca gcgtgaaccc gagaatgtct
    10441 gctgcctgca accaggtgtt ggccatgttg ttgccgtcgc cgacccaggc cacgaccttg
    10501 cctttcaggc aggcgagcgc ctcgcctgtc gaagcggcac cgccacggtg ctcgatgtag
    10561 gtgaacacgt cggccaggat ctggcacggg tggtactcgt tggtcagccc gttgatgacc
    10621 ggcacgcgcg agtgcgccgc gaaagcctcg atcttggtct gctcgtaggt gcggatcatg
    10681 accagatcga ccatgcggct gatgacgcgg gcgctgtcct cgatcggctc cgagcgcccg
    10741 agctggctgt caccggtggt caggttcacc accgagccac ccatctggta catgcctgcc
    10801 tcgaagctca cgcgggtgcg cgtgctcgcc ttctcgaaga tcatcgccag cgtgcgatcg
    10861 accagcgggg tgtacttctc gtagttcttg aagcgcttct tgatgaccgt ggtgcgggcc
    10921 agcaggtagg cgtactcctc tgcacgaagg tccttgaact ggaggtagtg ccggatcaat
    10981 gccttgccgg gtttcatgga gggtgttttc acgtcagtct ggcgagcgca ggaaatcctt
    11041 gatcagcgga cacagcgtca tcgcgatctg ctcggcttca gccagattca ggatcagcgg
    11101 tggtacgagc cgcacgacgc ggtcggcggt cacgctcagc agcagcccgg cctgggccgc
    11161 cttcagcagc aggtccccac agggacggtc cagttcgatg cccagcatca ggccctggcc
    11221 gcggatctcc accacgccgg cgacgccgga cagttcccgc gtcaggctgg cgcgcagcgc
    11281 ggcgcccacc gtggcgacat tctcgagcag gtgatcctcg cgcatgatgc gcagggtctc
    11341 gatgcccgca cgcatcgcaa gcgggttgcc accgaaggtg gtgccgtgat tgcccggttg
    11401 cagcacatcg gcagcgcgcg gaccgcagac gacagcaccc accggcacgc ccgaccccag
    11461 ccccttggcg agcggcatca cgtcgggcac gattccggcc cactgatgcg cgaaccactt
    11521 gccggtgcgc ccgatgccgc attgcacctc gtccagcatc agcagccagt cgttctcgtc
    11581 gcacagacgc cgcacggatc gcaggtactc gagagtggca gggcgcacgc cgccttcgcc
    11641 ctggatcact tcgaggaaaa ccgcaacgac gttcggccgc gtgttagcga cctcctcgag
    11701 cgcggccacg tcgttcagtg gcacacgcac gaagccctcg accagcggac cgaacccctc
    11761 ctgcaccttc gggttgccgg tggccgacag cgtggcgatc gagcggccgt ggaatgcctt
    11821 ctcgtacacg atgacctcgg ggcgatcgat gcccttgtca tgcccgaact tgcgcgcgat
    11881 cttcagcgcg gcctcgttgg cttcaaggcc gctgttgcag aagaaaacgc gttcgagtcc
    11941 cgacaattcg cacaaagtcg ccgcgaggct ttcctgcaat ggcgaggcgt agtagttcga
    12001 ggtgtgtatc agcctgccga tctgttcctg caatgcgggc accagtttcg gatgcgcgtg
    12061 acccagcgta ttgactgcaa tgccgccaag tccgtcaagg tacttcttgc cgtcggtgtc
    12121 ccatacccag cagccgcgac cgtgcgacat cgccatcggc aggcggccgt aggtgtgcat
    12181 gacatggggt tcgggatcga gagcggctgt ttccggggcg ttcatggcgg gactccgctg
    12241 tgacgaagga cgcattgcag gcttgccttg cgccagtcca agcgtcgaat tctacggtgg
    12301 ggcctcgtgg caatgagctg ccagaacgcc cccgtgtcgc ggctgatcgg cgagattcgg
    12361 catccaactt gctgccccgc agcaagcagg cggcacaatc ggcgcgcgcg catcgaatcg
    12421 atgaccgggt cgggggccgt gaggtcgcgc ttcggtcaca gaattctcat ccccactctc
    12481 gcagcaatga gttcatcccc tcctcgcgaa gtattcattc aaggcatcac gctcagcggg
    12541 cagacgttcc gacccagtga ctgggctgag cggctggcgg gcgcgatgtc gtcttttcgg
    12601 cccgaaggct cgaccgggga tcgctcgtcg ttcatcggct attcgcctta ctgcgtgcca
    12661 cgcgtgatca acggaatcaa gtgcgtcatc gtcgatgaac ggcttcgtga cgctgaaccg
    12721 atggcctggg atttcgtcat ggcgttcgcg cgcgacaacg aacttcaggt ggccgatgcc
    12781 tgcctgctcg atcctccgaa atcgaagccc gagcaatgaa ggctgcctgt gcggccacca
    12841 atgcaaaagg cccgcagtgc gggccttttc ttatgcgtcc aacccggaag aatcaggcgg
    12901 ttgcgggcag cgccttgatc ttggccgaca accggctctt gtgacgagct gccttgttct
    12961 tgtggaacag gcccttgtct gccaccgaat cgatgacctt ctcggcggac ttgaacaagt
    13021 cggccgcctt ggccttgtcg ccggcgatga cagccttctg cacgttcttg atgaccgtgc
    13081 ggaacttcga ccgaagcgcg gtgttggccg cattcagctt cacgtcctgg cgcacgcgct
    13141 tcaggcccga ggcgatgcgg acggttttct tcttggcttt ggatgaagtg gccatgcttt
    13201 tttgagtgtg cagttggagc tgagcccgcg agtatagcag cctgttgttc gccgcgggca
    13261 acctgtactg cagatgtgga cgagcgtgcc ggccccgctc cctatactgc ggcgacgcct
    13321 gtgcagcgcc ctggctatcg tgccgcatcc tccctgatcc catgaacctg ctgcgtgccg
    13381 cctccaccgt ttcgctgctg acgctggcat ctcgcatcac cggactgggt cgggagttgc
    13441 tgatcgccac gagtttcggc gccagcgctt ccaccgacgc gttcaacgtc gcgttccgta
    13501 ttccgaacct gctgcgtcgc ctgttcgcgg aaggcgcttt ctcgcaggct ttcgttccga
    13561 tcctggcggc cacgcgcgcc agcgagggcg aggcggcaac ccatcagctc gtcagtgccg
    13621 tggccacggt gctcttgtgg gcgctcgcgc ttgtcagcgt catcggtgtt gcgatggcgc
    13681 ctgtgctggt gtggctgatg gcgtcggggc tgcaggaatt cgatcaggcg gtcgtgatga
    13741 cgcgggtgat gtttccgtac atcgcattca tgtcgttggt ggctctggcc gctggagtcc
    13801 tgcagacctg gaaacggttt gccgtgccgg cagccacgcc ggtgctgctg aacctttcgg
    13861 tgatgctggc tgcctggctg ctggggccgt ggtttcagcg ccacggcatc gagccggtct
    13921 atgccctggc cgccggcgtc atgctgggtg gcgtcttgca gctggctgtt cagtggccgg
    13981 cgctgcgcgc gatcggaggc ttgcctcgca tcggcctgac ctggagtgct ctgcgcgttg
    14041 cgatgcagca tcccggcgtg ggccgcatcc tgcgtcagat ggcgccggcc ctgctgggcg
    14101 tgtcggtggc gcaactgtcg ctgttgatca acacgcagat cgcctcgcat gtcgctgtcg
    14161 gcgctgtgtc gtggctgacc tacgctgacc ggctcatgga attcccgact gccttgctgg
    14221 gtgtggcgct tggcgtcgtg ctgctcccac agctgtctgc cgcccaggcc agcggcgacg
    14281 aggctgcgta ttccagtcat ctggattggg gcctgcggct ggtgctgctg ctcgccttgc
    14341 cctgcgcggt cgcgctgctg gtgtttccga agccgatggt gtcggtgctc tatcactatg
    14401 ggcagttcag tgagcgcgac gtgcagcaga ccgtgcgggc gctgatgggc tacggcgtcg
    14461 ggctgatcgg gctgatcgga gtcaaggtgc tggcgccagg cttctacgca cgccaggaca
    14521 tccgcacgcc ggtcaagatt gccatcgtcg tgctcatcct cacgcagttg atgaacctgc
    14581 tgttcgttcc ccggctcggc catgcggggc tggcgctctc gatcggcctc ggtgcgttga
    14641 tcaatgccac ctggctgttg atcgggttgc gcaggcaggg cacgtatgtg ccgcaggctg
    14701 gctggctggg tttcagcttg cgcgtggtct gcgccagttc gctgctcggc ctggctctcg
    14761 gcgctgccgc ggtcggcatc gactgggtcg gactgtcctc gcagcgcttg cttcgggtcg
    14821 ggctgctggc cgcctgtctg ctcggcgtgg gcctggtcta tttcgtggcg ttgcggctga
    14881 tgggcatccg cctgaaggac ttcgcgcggc gcggatgacg gtgtcgcggc ggcgcgcggc
    14941 accgccttac acttgggaaa tgcccggacc gtatcagtac gcgacgccta ctgcgctcga
    15001 atacttctcc tcgttggtgg ccgaagatgc cagcctgtcg ctgctggagg cagctgcctc
    15061 gattgcccag gacgagcacc cgcggctcga cgttcaggcg gtgcttgccg aggtcgacga
    15121 gcttgccgtc aagctcaagc gtcgcatccc ggccgatgtc gttccattgc agcgcctgcg
    15181 ctggttgaac cgctattttt tccatgagct ggggttcggc ggcaacgtga acaactacta
    15241 cgacgcgagc aacagctacc tggatgacgt gctccgcacg cgccgtggca tcccgatctc
    15301 gttggcggtg atctacctcg aagtcgccac gcagctgggg ctgcgcgccc agggaatttc
    15361 gtttcccggg cacttcctgg tcaagctcag cctgctgagc ggatcgcaca atggtgaagt
    15421 gatcatcgat ccgttcaccg gtaaatcgct gtcccgggaa gagctcgacg agatgctgac
    15481 tccttacaag cgcagccagg gactggtcgg cgaattcgat gcaccgctgg gcttgttcct
    15541 gcaggcagcg gcgccgcgag agatcctggc gcgcatgttg cgcaacctga aggagatcca
    15601 cagcacggcg gaagactggc cgcgactgct gcacgtattg aaccgactcg tggtgctgtt
    15661 gccccacgcg cacgaggagc gacgcgatcg cgccctcgtg tgggccgagc ttgggcaggc
    15721 agacagggca gccgaggact tcgaggctta cctgcaccac cgccctgacg ccgacgacag
    15781 cacggcgatg gccgaacggc tcgaacgcat gcgccggggc gagcagcccc gactgcactg
    15841 aagcaggtgt gggtgtcacg gtgcatgtcg gcgcgatcga tcacgcgggg ctgcgcgaca
    15901 tgaagcagat tccgttgccc attccatctg gcagcacccc gacgttcgat tccttcatcc
    15961 ccggcagcaa cgcgatggtg ctcgctcacc tgcgcgacct ggggacacag cctgccccgc
    16021 tctatctgtg ggggccgcaa ggcagcggaa agacgcatct gctgcgcgcg ctggcgcatc
    16081 agacgcaggc tcaaggcggt cgggtcggct ggtttgatcc ctccgagccc gcaccgtggt
    16141 tgcacgagga atcgtggagt ctgatcgtgc tggacggttg cgatgcgttc gacgccgcgc
    16201 agcagcacgc ggcattcgcg ctgttcgtcg aagcaaccac gcacgggaca ccgctggttg
    16261 cggcagggcg gcttccgccc gtcgacctgc ccttgcggga tgaccttcga tcacgtctcg
    16321 gttggggcca tgtcatggct gtccacccct tgtccgaggc cgaatcgcga ggggccttgc
    16381 ggagagaggc cgatggtcgg ggcctgtttc tctccgacga agtgatggat cacctgctgc
    16441 accgccacgc cagggacatg aagcacctga tgacccagct cgatcggctt gacgagttcg
    16501 cgatggtcca caagcgcgcc atcaccgttc cgcttctgcg gcagatgctc tccgaagcgt
    16561 cgggcgacgg gctcaccgga acgctgctgt gaacctgtgt ctttttgatc tcgatgacac
    16621 cttgctcccg ctggactccg accacgcctg gggcgaattc gtgatttcgc tcggctgggt
    16681 ggatgaagac agcttccggc gcaccaatga cgctttcttc gcccagtacc aggcaggaca
    16741 gctcgacgtg cacggctaca tcgagttcgc cacccggcca ttgcgcgagc gcccggccga
    16801 tgagctggcg gctgcccaca gtctgttcat gcagcgtgtc atcgaaccgc agttgcgcga
    16861 cagcgcccgc gatctggtgc gccatcacca ggcaacgggt gatcgcgtcg tgctcgtgac
    16921 tgcgaccaac gaattcgtga ccgccccgat cgcggcggcg ttcggcatcg agtcgctgat
    16981 cgctgtccgc ctcgaacggg atgcgcgcgg tagcatcagc ggccgcatcg agggcacgcc
    17041 cagctaccgg gagggcaagg tcaggcgcgt cgaggagtgg ctgggcgagc agggcctgtc
    17101 ctgggccgat tgcgagcgca tcaccgtcta cagcgactcg ctcaacgact tgccgctgct
    17161 tgaaaaagcc accgatccgg tggcgaccaa tccgtctcct gcgctgcttt cggtcgccaa
    17221 tgagcgcggc tggcgcactc tgaacctgtt cacatgatca agaagtttct cgacaagctg
    17281 ctgggcaagc ccgccaagaa gaccgatggc gcttcctcgc tcggcaagcg ggccgaggtc
    17341 tccaagagcg agcaccagat cgatccaggg ctggtcgacg agcgcgcatc caaggtggtg
    17401 cagacgttgg tcgacgctgg ctacgaggcc tacatcgtcg gtggcgcggt tcgcgacctg
    17461 ttgctcggcc tgcgtccgaa ggacttcgac gtcgcgacca acgcaacccc ggagcaggtg
    17521 aagtcattgt tccggcgtgc attcatcatc ggccggcgct tccgcatcgt gcatgtggtg
    17581 tttggccgcg ggcgtcagga ccgggggctg agcgaggtga tcgaggtctc gacctttcga
    17641 gcgctgatgg atccggcggc tgccgaacag gtgaccggca gcgagaagac gctcaaggcc
    17701 gaaggggcgg gccggcatgc cggcccgagc catgtcgtcg atgccgaagg tcgcgttctg
    17761 cgcgacaacg tttggggtcc gcagatcgag gatgctgcgc ggcgcgattt caccgtcaat
    17821 gcgatgtact acgacccggt ccgcgaggtc gtcgtcgatt accacggcgg catcaaggat
    17881 gcaaagaaga agctgctgcg catgatcggc gacccggaga cccgctatcg cgaagacccg
    17941 gtgcggatca tccgagcggt gcgcttcgca gccaagctgg ggttcgagat cgagccgaag
    18001 acgcgtgcgc cgatccagtc catgtccacg ctgctggaga acgtgccgcc gtcgcgcctg
    18061 ttcgacgaga tgatcaagct gctgcagacc gggcactcgc ttgccagcat cgccgagttg
    18121 cgcaagcagg gcctgcacca aggcactttc ccggtgctcg atacgctgct cgatgagagc
    18181 cgcggtagtg cggcgcatcg caagttcgtc ggtctggcga tggccgacac ggatcgccgc
    18241 gtttcggaag gcaagccagt ggcgcccagt ttcatgctgg cctgcatgtt ctggcacgac
    18301 gttcagtcgc gatggaatca gttgaagcac gctggcgagc cggctttccc ggcgctgcag
    18361 caggcggtcg aggccatctt cgaagctcgc atcggtgaca tctcgggccg tggcaagctc
    18421 ggtggcgaca tgcgcgagat ctggctgatg cagccgcgtt ttgagcgtcg cgttggcagc
    18481 ggaccgttca cgcttgtcga acaaccgcgc tttcgagccg gtttcgactt cctgcgcctg
    18541 cgtgcggacg cgggcgaggc cccggacgaa ctggctcgat ggtgggaaga tttttccctg
    18601 gccgacgagg acgaacgtcg tcgcctgatg gacgacgcca aggtgcacga cggcaagaag
    18661 cgtcaacccg cctcggctgg cgaggcgcct ccggtcgccg accctgcaaa gaagcgccgg
    18721 cgccgccgtc gcaagccgac cgaagcggga ggctcggctc ctgccgacgg cgagaacgag
    18781 gcggtgtgac cgaggccagg gcctacgtgg gcctcggcgc caacctgggt gacgcgcggg
    18841 cggtgctggc ggcagcctat gccgaagtgc agcgcctgcc gcagacttct cgacatcgat
    18901 gctctccctg gttccgcacg gcgcccatcg attcgagcgg ccccgattac atcaatgcag
    18961 tcgcctgttt cgacaccacg ctgacgccgc aagacctgct ggaggcattg cagcgcatcg
    19021 agaacgccca cggacgcgag cggccctacc gcaacgcacc gcgcaccctt gatctcgacg
    19081 tcttgctgtt cggcgaacag acgatagcga cgccgacact ttccgttcct cacccgcgca
    19141 tgcatgagcg agcctttgtc ctgcagccgc tggcgtcgct ggcgcccgag gcggtgattc
    19201 ccgggcgcgg cccgctcagt gcactgcttg cgctggtgtc ggagcaagct atcgagcggc
    19261 tggactgaca actgcaggct ctgagtcgac ctggttctcg aaccagcgct gatccgcgag
    19321 tagatcacgg tgcagaggtg accaggccac gtacaaatgg aggtgacggc cggcaggctg
    19381 aaagcagatc ctctgccgcg atgtcacgtt gttgcgtatc cgggcggcac acggtttaca
    19441 atcgagggtt cacgtgacgg ctgactcgcc cacgctgctc ccggattttc tggttgcagg
    19501 gcctgtcggc gtatctacgg cactgatctt gttgcggccc gacggccgca gatttctgtt
    19561 ctgctcgacc ggagtgtctc ccggatgcgg cgggcaaccc atcattcaag gaaacatcgt
    19621 ggttgtgatt cgactgtccc gtggcggcgc caagaagcgt cctttcttca acatcgtcgt
    19681 tgccgattcg cgagtccgtc gtgatggccg cttcatcgag cgcgtcgggt tctacaaccc
    19741 ggtggcctcc ggtgccgagc agccgttgcg ggtcgccttc gaccgcatcg cctactggac
    19801 cggtaccggc gcccagttgt ctcccacggt ggcccgtctc gtcgagcagg ccaaggccgc
    19861 cacggtctga gtgattgccg gctcggcggt cgcctgatct cgcgacggcg ccgccgatgc
    19921 cgatgactgc ctcgcaaacc ggcatggcgg tgcaatccga ttcaggcttc gacgcggtgg
    19981 cctggcccga tgacgccatc gaggtcggcc tgatcgtcga cgcctggggt gtcaagggct
    20041 ggttcaaggt caaggcctat tcgtccgatc cccaggcgct gttctcttcc cgccgctggc
    20101 tattgaaggc gccggaagta cccggaccgc gaacgttggg tcacaccctg cagttgcccc
    20161 cgttgctgaa gatcacggcg gccaaggaac acggtgacgt ggtcgtcgcg caggcgcagg
    20221 atgtggtcga tcgcgcaggg gccgaagcgc tgcgcggtgc tcgcgtgttc gtgtcccgag
    20281 gcagcttccc gtcagcaggc ccggacgagt actactgggt cgatctggtc ggtctgtcgg
    20341 tcttcaaccg gcagggtgaa ttcctcggcg acgtggtcgg cctgatcgat accggcccgc
    20401 acagcgtgct gcgcatcgct ccgaccgggg tgactgatga agctgcgcag cgtttgattc
    20461 cgttcgttgc agcctttgtc gattcggtga gcctgaccga ccggcgcatc accgtcgact
    20521 ggggcctcga ttactgagcc tcggtcgact gcatccatgc gattcgacgt catcacactg
    20581 tttccggagt tgttcaagcc gcatctgatc catgggatca cgcggcgcgc ctacgactcc
    20641 gggcaggtcg acgtgcgcct gtggcctttg cgcgactttg cggacgggca gtaccggcgt
    20701 gtcgatgatc gcccctacgg aggcggcccg ggcatggtgc tgatggccga gccgttggag
    20761 cgggccttgt cggcggtgcg tgctgcgcgc gcgtctgacg tctccgaact gacaccggtg
    20821 ccagtcatcc atttcacccc gaccggtcgc cggcttgacc aggccgtggt ggctgaaatg
    20881 gccgccggcc ccggtgccgt cttgttgtgt ggccgctacg agggaatcga ccagcgcttc
    20941 ctggatcggc atgtcgacat cgagctcagc cttggcgatt tcgtgctttc ggggggtgag
    21001 ttgccggcgc tggcactgct ggatgccgtg gcacgactgc aggatggcgt cctgggcgat
    21061 gcgcagtcgc accagcagga cagcttctcc gaggggctgc tcgaggggcc gcatttcagt
    21121 cgccccgagg tattgagcac cgacggtggc ctgttgccga tcccgcctgt gctgctgtcg
    21181 ggtcatcacg gacagattgc tcgctggcgc cgggagcaat ctctggcact gacggcgcgt
    21241 cgccgccccg acctcatctc ggctgcgaga gccgccaagc gcctgacggt cgatgacgag
    21301 cgttttctgg ctggactcga cgtatactag agggcttttc tgatcctctg ccacgcccgg
    21361 ccgcactgcg ttgaatgcat tcagactgcg tcacgctgca ggtccggaca aattcctcca
    21421 ccaagcgtgg gcaagatcac acggagaaac catggacctg atccaaaccc tcgagcaaga
    21481 ggaaatcgcc cgtctgaaca agaccatccc tgccttcgcg ccaggtgaca cggtcatcgt
    21541 caacgtgaac gtcgtcgaag gcacccgcaa gcgcgtgcag gcctatgaag gcgtcgtgat
    21601 cgccaagcgg aaccgcggcc tgaactccag tttcatcgca cgcaagattt ccagcggcga
    21661 aggcgtcgag cggacattcc agctctacag cccgctgatc gcatcgatcg aagtcaagcg
    21721 ccgcggcgat gtgcgccgcg ccaagcttta ctacctgcgc cagcgctctg gcaagtcggc
    21781 tcgaatcaaa gagaaactgg cctgagcgcg tgcgccagcc ttgcgcatga cgcaaggcgg
    21841 cgcacaggcg cccagccggg tctttggcgc gacatggccg agcttgccgc ggcaactggt
    21901 tggtctgcca gtcgcagctg accgaaagcc gcctttgagg gcggcttttt caatttcgaa
    21961 gatccgatgc cgccgacctt cgacccgcaa caactgccca ttctggcgat tgacgatcac
    22021 ctgccggcgg tgccgtccga aaggctgaca gccactggcg ttcgcgagcg ttttgcgcgc
    22081 tctacggtct ggcagcccga actggtggtc gaacgcgtgc tgcgccaacg ggctctcgtc
    22141 cacgcgtctg tgctggtgcc gcttgtcgtt cggggcgatg agctaaccgt gctgctgacc
    22201 cagcgagccg accatctcag cgatcacccg ggacagatca gctttcccgg cggccgcgcc
    22261 gaagttggcg acctgacggc ggtcgcgacg gccttgcggg aggctgagga agaaatcggc
    22321 ctggcagcga agcatgtcca ggtgctcggt acgctgccga cctacaccac cgtcaccggc
    22381 ttcatcgtca cgcccgtggt gggactggtc gagctcggct tcgagttgcg cgtcgacccg
    22441 gtcgaggtgg ccgacgtgtt cgaggtgcct ttgaccttcc tgatgtcgcc gggacaccac
    22501 cgtcgtcatg cggtcgggga gggcgacgat gtgcgcgagt tcttctcgat gccctggcag
    22561 gcctcggtgg acggcggtcc accgcagtgc cgcttcattt ggggagccac tgcagcgatg
    22621 ctgcgcaact tctaccgttt cctttcggcg tgagccgcgc cggatgcggc cggtatcatc
    22681 gaatgcgatg agtcttttct cggtactctt tgcgctgctg ctcgaacagc tcaagcccat
    22741 gcagcgcgga acggccatcc atgcggccct gacttcgtgg ttgcgatggg ccggccgcaa
    22801 tttcgatgcg ggccgtgatc gacatgcctg ggttgtctgg ggtatcacgg tggtgcttcc
    22861 ggcgctgttc gcgtttgccg tacacgcggg tctggcgcat tacagcctgc tgctcggtct
    22921 gatctggaat gtcgcggtgc tctatctgac actgggcttc cgccagttca gccattactt
    22981 caccgacatc cgcgacgcac tcgaccgcga ggacgagcct gaagcgcgcc gcctgctgac
    23041 cgagtggcga cacctggatg tcagcgaact tcctcgcacc gagttgctgc gacacgtgat
    23101 cgagcactcg ctgctagctg cgcaccggca tgtgttcggg gtcttcttct ggttcgtcgt
    23161 gctctcggcg atcggttttg gtcctgctgg cgccgtgctt tatcgcactg ccgaattcgc
    23221 aagccgttac tgggcctacc gcagccccac cgtcggcgaa ccgaccaatg aacgattgat
    23281 ggccttgtcg catcggatgt tcgaactgat ggatcacttc ccggcgcggc tcaccgcatt
    23341 cggtttcgcg gtcgtaggca acttcgagga agcgatcaat tgctggcgcc gtgacgccga
    23401 actgtggcga cacccgaacg aaggggtcat ccttgccgcg gccgccgggg ccgtgggtgt
    23461 gcaactcggt ggcacggctg ctccgggtgt gacgcccgac cgatcgaaga cctttgttcg
    23521 cggagtcggt gtcgacgatc tggctgctga cggatcgacc accgggatgg cgccggtgca
    23581 ggggcacctg cgaagcatcg tcggcctggt gtggcgatct gtcgtgctgt ggatgatgct
    23641 gctggccctg ctcacgctgg ccaatctggt cggctgattc tttcaacgcc taccagcgcg
    23701 tgccttccag ttcctgcctg atctcgccgt agatgcggta gcgcgactcg ctgatctcac
    23761 cctgtgccag ggcttcgcgc acgccgcagc ccggctcctg ccggtgcgtg cagttgtaga
    23821 agcgacagcg ggtcgaagcg tctcgcagat cgggcatcag cgccggcagt ttctgcggat
    23881 cgacctgctt gaggccgaac tcctggaaac ccggcgagtc gatcagtccg gtggatcgga
    23941 ctgcatccag ccagtaccac tgcgtggatg tcgtcgtgtg ccgcccggaa ttcagggcct
    24001 gcgacacttc accgacttgc gcgcgggcat ccggcgccag cagattgatc agcgtgctct
    24061 tgccggttcc gctgggtccc agcaccagcg aagcccgtcc ggccagcagt gggttcagga
    24121 tggcgcggga tgcctcgggc tgggccttca acgcgacctc gagcacgcgg tatcccatct
    24181 cgacataggg cttcaatcga tcgcgcgcgg caacgatctg cggcaggtcg gccttgttga
    24241 gcaggatgct ggcggcgatc ccggcgctct ctgcggcaat cagcgcacgc gcaagttgcg
    24301 actcgctgaa caccggctcg ctggccacca tcaccaggac ctgatcgagg ttggcggcga
    24361 aggccttggt cttccattcg tcctgcctga acagcaggtt gcgtcgcggt tcaagttcct
    24421 cgatgactcc ttcgtcgccg gtgatctgcc agcgcacgcg gtcgccgacc acgcacaggc
    24481 tcttcttgcc gcgcggatgg cagatggtcc gttcacccga ggcggattcg acgatgtagt
    24541 ggcgaccaaa tcccgaaacc accagacctg aagtggtcgg ggcatcgccg accttggcct
    24601 tgctcaaggt gtcggtccgg tcatgcggtg ggtgccagcg cccgcatcgc cgccagccgt
    24661 tcaccggccg gggggtgcga gtagtagaag cgggcgtaca agggatcagg ggtcagcgtc
    24721 gatgcgttgt cttcgtagag cttcagcaag gccgccgaca ggtcacggcc gtcggatttc
    24781 gagcaggcat aggcgtcggc ctcgaattca tggcgtctcg acagtccggc gagcagcggc
    24841 gacacgaaga agccgaacac cggcacgatc agcatgaaca gcatcagcgc cagcgcgtcg
    24901 ttgggcgccg acatcgaagg ctgcacgccg aatgcggtgt agaaccagat gcggctcgac
    24961 agccagccca gcgcggcgaa gccggccagg ctgatcgcga acatcgagac gattcgcttg
    25021 agcacatgct tgtgcttgaa gtggccgagt tcatgcgcga gcacggcctc gacctcgctt
    25081 ggcgacagct tgtcgagcaa ggtgtcgaag aagaccaccc gtttcgaagc gccgaagcct
    25141 gtgaaatagg cgttggcatg cgccgagcgc cggctgccat ccatcacgaa gaagcccttg
    25201 gcggtaaatc cgcatttcgc catcagcgca tgcacgcgtg cttcgagcga tgcgtccgca
    25261 agtggcttga aagtgttgaa caacggtgcg atgagcgtcg ggtacagcac cagggcgagc
    25321 aggttgaagg ccatccaggc gccccaggcc cacagccacc acagctggcc tgtgctgccc
    25381 atgatccaaa gcatcccggc cgccagcggc agaccgatga gggcacccag cagcaggccc
    25441 ttgaaggcat cgatgacata cagcttccag gtcattcggt tgaagccgaa ggcctgttcg
    25501 atgcggaagg tttggtagat ctcgaagggc aggtcgagca gcgcgcctat ggcaacgaag
    25561 gtgccgatca aggcgacttg gtaagccatg ttgccgaaaa gtggctggac cgtgtcccga
    25621 accgctgcgt tgagcaaccc gagtcctccc agcagcgtcc atccgaccag cacgactgca
    25681 ctgaatgcca tcgtccacag accaaaactg cccttcgtca gggtgtagcg agccgccttg
    25741 cgatgggcct ccagcgagac ggtgccggcg aaagccgcgg ggaccgcatc gctgtggatg
    25801 gctacgtggc gcatctgccg ggttgcgagc cagaagcgga tgagcaacga gaagacgagt
    25861 gccgccgaga acagcagaac gagcaccagc gcagggctgg tggggaagac ggagtaattg
    25921 ggcatcggta ggcagtgtag gtcggcggga acactcggac agcggtcgta agacaatcac
    25981 cgcaaggagt tcatgacatg actgaaacgc ttggcaagag tgaccagaac ctgatctgga
    26041 tcgacctcga aatgaccggc ttgttcccgg ataccgaccg catcatcgag atcgcagtca
    26101 tcgtcaccga ttcacagctg aaagtgcggg tcgaaggacc gaccatcgcc gtgcatcaga
    26161 gcgatgccgc gctcgacgcg atggacgcct ggaacaaggg cactcatggc cgcagtggcc
    26221 tgatcgatcg cgtcaaggcg tcctctgtcg atgaagaggc cgccgaggcg cagatcatcg
    26281 ccttcctgca gcagtacgtg cccaagggca agtcgcccat gtgtggcaac agcatctgcc
    26341 aggatcggcg gttcctcgcg aggacgatgc cgacgctgga ggcgttcttc cactaccgca
    26401 acctggacgt cagcacgctg aaggaattgg ccaagcgctg gaagcccggc atcctggacg
    26461 gattcaagaa ggctcaggcg ca
//
LOCUS       DAOORV010000039        26319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2213_length_26319_cov_9.275015, whole genome shotgun sequence.
ACCESSION   DAOORV010000039 DAOORV010000000
VERSION     DAOORV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26319
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_26319_cov_9.275015"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PKV56_07290"
     CDS             <1..121
                     /locus_tag="PKV56_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67656.1"
                     /translation="YDFAAELADQYLVMERGEIIQRGRGKDMEAEGVRARMSI"
     gene            complement(212..889)
                     /locus_tag="PKV56_07295"
     CDS             complement(212..889)
                     /locus_tag="PKV56_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HOX67657.1"
                     /translation="MGKLEEQIHAIQRRRFLRYAGATLGGAAAGLPTLQATAAECRLT
                     EQEILGPMYNFGAPMVQLKLAADDEPGQKLMLRGSVLSADCRTPLAHTLIEIWQADDK
                     GVYDKQQPGNFLESVKPHLRGMVLTDAKGRYEIETIVPGAYPIPPGVPGLEKYGNLTR
                     ARHIHIKVEPFLHTALTTQLYFKGDEHLAGDPWGGHKPGLALAMKQDGRYMKADFDFV
                     LGTGLKG"
     gene            complement(1035..1865)
                     /locus_tag="PKV56_07300"
     CDS             complement(1035..1865)
                     /locus_tag="PKV56_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOX67658.1"
                     /translation="MGWPGRLELNYRRAEDRTLAHDRHSGPLRVLQSLYPEGPGICHH
                     VLVHPPGGIVGGDVLHVEATLAPHAHALITTPGATRFYRSAGEAARQSVTARVEDGAR
                     LEWLPLETICHSAALAENSMRFELSPGGEMMGWDVIALGLPASNDSFDSGHYMQRIEL
                     PGVWLERGTIRGDDKRLLDSPLGFAGHRVLATLWFAAGSPMAPDRRDTLLDAARELAG
                     AHALARTAGCTSPHAEVVVLRVLAPRVEPAIQLLAGVWARWREIGWGLQPCVPRVWRT
                     "
     gene            complement(1883..3691)
                     /locus_tag="PKV56_07305"
     CDS             complement(1883..3691)
                     /locus_tag="PKV56_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67659.1"
                     /translation="MTAEPMAAAGEAPEASLPEEVRSAFDYMPAQLAGNLAGIGVIVL
                     LFWAVTPASVMLPWLGLFAVLWLARLSLLQRFNRVAALGVVDWARWRLWWNLGTLTSG
                     ALWGATSWVFYLLGGNEQQIALIITIYTFCIAVVPVLATQPRVFLLFSALCFVPMAAR
                     VASDGSEESFRLAGILLLIFSLTTLLARNYRQALQRALALKLQADHLLSQLRLEKTAA
                     DTSRQQAEIANRAKTQFFTAASHDLRQPLHAMGLFAEALRQKNHDVEVAHLVNSINES
                     VDALEGLFSELLDITRIDSGGVEVHPQHFAIKDLYRKLRLHFEPLAFEKGLALRFRGS
                     QHVALADPLLVERIMRNLVSNAIRYTVDGSVLVSCRRRGDRLMLQVWDTGPGIAEGEQ
                     AKVFEEFYQVPNGPRTDPHQRKGLGLGLAIVKRLADLIAAPVALSSQPGKGTVFTLEL
                     PPGKRMPQAQPMLSVKGPLGITLDGRLIVVVEDEPAVLSALEALLSGWGAGIAAFTSV
                     AACAAWARGADTQLVKPDLLIVDYRLEQGANGVDAIAVLRARFGEHVPAIVITGSTMS
                     ALDLEAQQKDFHLLIKPVVPNKLRAMIAFKLGVKSA"
     gene            complement(3688..4419)
                     /locus_tag="PKV56_07310"
     CDS             complement(3688..4419)
                     /locus_tag="PKV56_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67660.1"
                     /translation="MKVLLIDDHPLILSALQTIIQGLGENVSVVGAGSAKAARDTLKQ
                     NSDFDLALLDLRLGDADGFDVLTEFRAAYPAMPVVVVSASDRTSDVIRAIDLGAMGFV
                     PKRSSNDVLFEALSMVMSGGIYVPPMNMGDESAGADLYSDSTPGALRSVRQSAADEGY
                     QSHAPLESLGLTPRQTEVLGLLLQGKPNKLIARDLKLSVETVKDHVAAVLRTLNVSSR
                     TQAVLAVGQMSRQQGGLHSWRKSGP"
     gene            complement(4531..5148)
                     /locus_tag="PKV56_07315"
     CDS             complement(4531..5148)
                     /locus_tag="PKV56_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOX67661.1"
                     /translation="MPVIVVANPKGGVGKSTLSTNVAGYFAAQRKAVMLGDVDRQQSA
                     ASWLAQRPETAASIRAWDTGADLRVRPPKGTTHVVLDTPAGLHGKRLDEILKIATKVL
                     VPLQPSIFDIRATHDFLHRLAEHKRAQKLEVGIVGMRSREGTISADQLKAFLGGQEFP
                     VLTHLRDTQNYIHLAAQGLTLWDVAPSRVERDLEQWQPLTAWLNA"
     gene            5438..5896
                     /locus_tag="PKV56_07320"
     CDS             5438..5896
                     /locus_tag="PKV56_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOX67662.1"
                     /translation="MERHTRQRQAVLDALVRSGQALSPQDLLGQAKSAVPSLNLSTVY
                     RQIKGLQDAGQIVRVELPGQPPRFEMASRGEATAPHRHAAHLVADQRHAPDEASRHHH
                     HFHCVQCDRVYPIHACPGPMNDLAPPGFEVQHHDLTLSGRCASCVAGAPA"
     gene            5893..6159
                     /locus_tag="PKV56_07325"
     CDS             5893..6159
                     /locus_tag="PKV56_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67663.1"
                     /translation="MKAGNMDAALHVHAAGHGHGHAHGHDHSHDDGHDHAAHAAGSSL
                     LIPKPAPAPVSSLLMASALLRFAGAIGLIALLWMAVVWAMSGTA"
     gene            6156..6971
                     /locus_tag="PKV56_07330"
     CDS             6156..6971
                     /locus_tag="PKV56_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67664.1"
                     /translation="MNGIDVPALPNSTQLPVVAPPAIAVEDLTVSYRHHPAVHHVSCR
                     FEPGSLTAVIGPNGAGKSSLLDALTGRVQPSTGCVRTGGLPRNRLAYMPQQSGIDRTF
                     PLGVLDVVMLGAWPRIGWFTGATTAQRAQALLALASVGLAGFEQRLISELSVGQFQRV
                     LFARVLMQDAPVILLDEPFNAIDTRTTADLMRLVQHWHDEGRTVIAVLHDLAQVREHF
                     PLTLLLARECVAHGPTEEVLVPRHLMRAQQMAEQWDENADWCAAPVLVSRAPS"
     gene            6968..7855
                     /locus_tag="PKV56_07335"
     CDS             6968..7855
                     /locus_tag="PKV56_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOX67665.1"
                     /translation="MTLLMWLQGMLIQPFVEFGFMRRALVACLALGLSCGPIGTLLVL
                     RRMSLVGDAIAHALLPGAALAFVLFGFSLWAMSIGGFVAAVAVAGLAGLVARLTPQRE
                     DASFAAFYLIALAAGVMIISIRGNSVDLMRVLFGTVLAVDDASLLLIAGIASLSLLVL
                     ALVYRPLVVECFDPGFLRHLGNAGAWVHALFLMLLVLNLVGGFQALGTLMAVGLMMLP
                     ATAARFWAAEVWSLAAVAALFAVLSGWGGLLLSYHANLPSGPSIVLLAGSFYALSLLI
                     GRRDSLLARQLSRRSHRKA"
     gene            7858..8826
                     /locus_tag="PKV56_07340"
     CDS             7858..8826
                     /locus_tag="PKV56_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOX67666.1"
                     /translation="MQFDSTPRALRRAFLTLLASAGTARWSVAPAAQTPPPVLKVVAT
                     FSVLADIAREVGGTAVEVTALVGPNADAHVFEPTPSDVRRVASADVVVTNGLRYEGWI
                     DRLIKASGFRGPVVVASQGVVTRGVSGGADPHAWQSLANGQRYAENIRAVLVAALSAR
                     APARVAEVEARAAAYQQRLAALDRDTRERFAALPAERRRVITAHDAFGYFSDAYGVVF
                     IAPRGWTTGSEPSAKAVAAIVRQARASKASALLVENISDPRLIERIGRESGLKVGGAL
                     YSDALSPVGTEADTYVRMFEHNVKTLVDAMSLNVPGASGDAGAVPR"
     gene            8848..9177
                     /locus_tag="PKV56_07345"
     CDS             8848..9177
                     /locus_tag="PKV56_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67667.1"
                     /translation="MNSRAFNSTWLREELLHLLSLSGTLAGFSVTAVTLLRTIGQPSL
                     MQTVADDVLAMSALLFLVCTYAIFIALRTQRQALARALEKLIDGLFVLALTGMVSAGF
                     IMVYTLW"
     gene            9349..10353
                     /locus_tag="PKV56_07350"
     CDS             9349..10353
                     /locus_tag="PKV56_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOX67668.1"
                     /translation="MSDSNPVSTESLSVLGIKTGRAGPRLLVLGAVHGNETCGPRAIE
                     KVLADFDVEKLKLLRGNVTFVPITNPLAYQRGRREGDRNLNRNLRPTDTPQDFEDRIA
                     NALCPLIAQHDMLLDLHSFQSPGEPFAMLGPLNNEDTLQPFELAAHEEAVAVRLGPKR
                     FVEGWLETYAAGVANRVKRADQSVDPNARATLLNTDPSYGIGTTEYMRSVGGVAITLE
                     CGQHDDPNAPEVGYRAILNAMAHLAMIEAPRPEPRTDVEVLRLTEVTDRLHADDAFVK
                     PWASFDPVSAGEPIGRRHDGTLVTAPSDGFIVFPNTKATPGNEWFYFARTSDRKLNVE
                     "
     gene            10420..10953
                     /locus_tag="PKV56_07355"
     CDS             10420..10953
                     /locus_tag="PKV56_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HOX67669.1"
                     /translation="MKRISAWVAALFLSTGTVVLAQGQPHVHGVARLDVAVEAGQVVL
                     QFETPLDSLLGFERAPRGADETRRAELAIAALKAADALFRFTPAAGCTPARVDLRSAA
                     LKLGSPDPAEAHEGHADLDATYVFKCVDSAKAAEVDVGLFEAFSRLQGLRVEVVTARG
                     QFKRDLKRPAKRVSLAR"
     gene            11024..11734
                     /locus_tag="PKV56_07360"
     CDS             11024..11734
                     /locus_tag="PKV56_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX67670.1"
                     /translation="MIEVRDLRFAWPGAASDCVVIDALSIPAGRTVFLHGPSGGGKST
                     LLGLLAGVLLPRVGSVSLLGTRWADLSGARRDAFRADHVGYIFQQFNLLPYLSAVDNV
                     LLPCRFSRLRRERAAQDGGSPGHAARELLERVGLSQALWTRAAAQLSVGQQQRVAAAR
                     ALIGRPEVVIADEPTSALDALLRDSFMDLLLDACHASRGTLVFVSHDERLAARFDERL
                     SLSAVNRTASSMQAEDSL"
     gene            11731..13005
                     /locus_tag="PKV56_07365"
     CDS             11731..13005
                     /locus_tag="PKV56_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67671.1"
                     /translation="MSALLTLAWRSSWSRRATLSLVLLSVALSTFLLLGFEQVRTDVR
                     ENFSQSVSGTDLVVGARTGSVQLMLYAAFHVGSATNNIRMDSLAAIAQHRAVDWVVPL
                     SLGDSHHGFAVLGTTSDYFNRFLYGDREPLVLAQGRAFTNGIDGLYGAVLGAEVAHAR
                     RYRLGDKLTLTHGSGGLPGSEHADKPFTVVGILARTGTPVDRTVHISLESLEAIHIDW
                     VGGAPLPGVVIAPEQARKFDLAPKQVTAALVGLKGRAAVFNVQRFVSGYAGEPLMAVL
                     PGVALDELWDVVGLGEKALLGMSAMVGVVSLAGLIAVVMAGLNERRRELAVLRAEGAG
                     PRHVLLLLAGEGLMVTLAGALLGALATGLFIATSAPWVQAHHGVNLKLSVPSETQWML
                     LAAVVLAGGVASLIPAWRAYRLSLADGLSPRV"
     gene            13008..13658
                     /locus_tag="PKV56_07370"
     CDS             13008..13658
                     /locus_tag="PKV56_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HOX67672.1"
                     /translation="MKNGSWVWGVAAATGLRAVATMVGVLLLGAAGSSWAQDGGAGAA
                     GSPSLSRPLGGSGASKAAAPGTFREIKWDDLVPQDWDPLKQFKSTNFSVMNDSDPRAT
                     EMLKRMRETWDNAPTNNEMDGVAVRIPGYLVPLEDNKSGLKEFLLVPYFGACIHSPPP
                     PANQIIHVKPQKPPKGFHSMDTVWVSGTLKTLRSDSYMGSSGYRMDAVSVEAYVEK"
     gene            complement(13668..14687)
                     /locus_tag="PKV56_07375"
     CDS             complement(13668..14687)
                     /locus_tag="PKV56_07375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX67673.1"
                     /translation="MRYSESKERTAELLRLALGHMGRHAAAFNPVTFTLWYEYVAGIN
                     PGLASSVDRLIKDESGIDDDEVVELYKRHIAPADEQTMKTISGEMERLMASMVQTASQ
                     TGERAGTFGDQLSGLNTALASRDVEQLKPRLTEVMAGTAEMKSSVEALRQKVTASQDE
                     INRLRSDLERARDDAILDPMTGILNRRGFEKRIESLLTNKPIDGTSHCLVMLDIDHFK
                     KVNDTHGHLMGDRVIQAVGEILRTSVTNGTHAAARYGGEEFALLLPQTSLDQSIQLAE
                     TVRTRIKAMKIRHRTTQEVLLTVTISGGVAEMRPDDDASSLIARADAALYQAKTGGRD
                     RVARA"
     gene            14852..15286
                     /locus_tag="PKV56_07380"
     CDS             14852..15286
                     /locus_tag="PKV56_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HOX67674.1"
                     /translation="MFAPSQNDVRRFFCAAHQKSRDGLPMTALEILAADWIAEHPEYH
                     AELADVDAALAAVYDVGAGRSNPFLHLSMHLSISEQVSIDQPRGIRQAVELLTARRSS
                     AHAAHHEVMECLGDMMWASQRSGLPPDGERYIASVRQRATRD"
     gene            15374..16084
                     /locus_tag="PKV56_07385"
     CDS             15374..16084
                     /locus_tag="PKV56_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX67675.1"
                     /translation="MDATQPTTVQRDAMWTLAFLAFVLAWDAGGGDLRVMHLFGDAHG
                     FAWRNHWLLVGWFHEGGRWLSALVVGVVLVNVVRPWGFARDMSTLTRWWWCAATLTCL
                     LLIPTLKHGSMTSCPWDLTEFGGSAHYVSHWARHIRDGGPGGCFPAGHASAAFSFFGG
                     WFALRATSPKAARRWLAGVWACGIAFGVAQSLRGAHYPSHSLWTAWLCWTTSALMWHA
                     SSRLRMMPMRRVEAVVGS"
     gene            complement(16100..16942)
                     /gene="panC"
                     /locus_tag="PKV56_07390"
     CDS             complement(16100..16942)
                     /gene="panC"
                     /locus_tag="PKV56_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOX67676.1"
                     /translation="MLIVHNIAALRAALPVPGTCTFVPTMGNLHDGHLALVRQAAAHG
                     LPVVTSIFVNRLQFAPHEDFDRYPRTLDRDCELLAPAGCNIVFAPDEHELYPEPQTFT
                     VQPDPALADILEGHFRPGFFTGVATVVLKLFNAVQPRVAVFGKKDYQQLMVIRRMAHQ
                     FALPIVIEAGETLRAHDGLALSSRNGYLSQAERAQAVELSQALHALTERFRDGIGPVD
                     LIESQARQALTARGWKPDYLTVRRQDNLLSPAESDDRRGNWVALGAARLGATRLIDNL
                     EFSI"
     gene            complement(16945..17781)
                     /gene="panB"
                     /locus_tag="PKV56_07395"
     CDS             complement(16945..17781)
                     /gene="panB"
                     /locus_tag="PKV56_07395"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOX67677.1"
                     /translation="MSSHPSNEAAPAGRKPVTLHRLREMHAAGEPISVLTCYDASFAR
                     LLDSAGVDCLMVGDSLGMVVQGRSSTLPVALEDAAYHTRCVARGNRSAWLISDLPFGS
                     YQQSREQALASATVLMQAGAHMVKLEGGGWTAETVRFLVDRGIPVCAHLGLTPQSVHA
                     LGGYRVQGRSDEAVALLLRQATELAQAGAAMMVVELVPSEVGRALTQTVGIPVIGIGA
                     GVDCSGQVLVLHDMLGVTQGRLPRFVRDFMKGAPSIEAAVATFVADVKARRFPDPALH
                     GY"
     gene            complement(17792..18802)
                     /gene="bioB"
                     /locus_tag="PKV56_07400"
     CDS             complement(17792..18802)
                     /gene="bioB"
                     /locus_tag="PKV56_07400"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOX67678.1"
                     /translation="MQATSPQEAPIQLVRNTAPAAASGAGTQRWQVAQIEALFALPFP
                     ELLFRAQTVHRANFDPTEVQLSTLLSIKTGGCAENCSYCPQSAAHDTGVEATALMKVD
                     EVLAAARQAKAAGATRFCMGAAWRGPKDRDIEKVSQLISAVKGLGMETCATLGMLGDG
                     HAEALRDAGLDYYNHNIDTAPDKYADIVQTRQFEDRIDTLQRVRGAGVKVCCGGIVGM
                     GETRTQRAALIAELANMEPYPESVPINHLVPVEGTPLHGQAPLDPFEFVRTIAAARIT
                     MPHTRVRLSAGRQALGDAVQALCFLAGANSIFYGDKLLVTGNPDVSADQVLLETLGMK
                     AA"
     gene            complement(18869..19690)
                     /locus_tag="PKV56_07405"
     CDS             complement(18869..19690)
                     /locus_tag="PKV56_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOX67679.1"
                     /translation="MADDVANIPPVVLDGVAVARLYGEPLFKLPHDLYIPPDALQLFL
                     EAFEGPLDLLLYLIRRQNFNILDIPLADVTRQYLTYVEQIRKTNLELASEYLLMAAML
                     IEIKSRMLLPPKRSPDGAEPEDPRAELVRRLVEYEQMKLAAARLDALPVLGRDFLRAQ
                     VHIEQSLVPRFPDVDPDDLRAAWADVLKRANLTQHHTINREQLSVREHMSIVLRALQG
                     RKFVEFHELFDVSRGQPVLVVTFIAMLELAREMLLEITQAEAYAPIYVRLAYQAV"
     gene            complement(19766..19969)
                     /locus_tag="PKV56_07410"
     CDS             complement(19766..19969)
                     /locus_tag="PKV56_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HOX67680.1"
                     /translation="MPLFWKPYKSDVTDFIETLKAKKPSLEAEQRAGRALLWDKQVDR
                     DAQAEDAESRVPQRPYVYQTGPK"
     gene            20141..21760
                     /locus_tag="PKV56_07415"
     CDS             20141..21760
                     /locus_tag="PKV56_07415"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOX67681.1"
                     /translation="MQEVELKFQVPPQARKAVESAVAGPHPARRIRLQAAYFDTPLGA
                     LAGAALALRLRKEGRVWVQTLKGTLPDGTGMIRSEHNVPRVEAGPDAPAIDPHLHAGT
                     PVGAALLKVLQQADGELRSVMVTDIWRRVRTTRVPGGWVELAFDVGTIEAGPAEAPRR
                     VSVCELEIELKRGHPQAVVGAAQRWVTRHGLWLDTRSKAELGNLLLRHVAMAAPRRAG
                     EVILARSMSPAQALDVVLRSCLEQISVNASQIASGVHAPEHVHQLRVGLRRLRTALRF
                     FGGSQLAEAIDAGTRDALAADAAEWFRLLGQARDRDAVAGPLAIDLARALSAVGLSGE
                     MPALPATGDEIDPTLVVRRGSSQRLMLGLISHLQRGQPTVAGAAVQVASAAAQPVVSE
                     PRIEDPMTSLTLRKRLVQRLNRWHRQVAADAQRFDALDDEGRHRLRKHVKRLRYAVEF
                     SAGVFDAREVRRYLQGLRALQERLGIVNDVAVGIALYSAAAPADARALFALGWLASQR
                     DRALQACRPELKRFLAMPRFWRSRRNKTASK"
     gene            complement(21792..23471)
                     /locus_tag="PKV56_07420"
     CDS             complement(21792..23471)
                     /locus_tag="PKV56_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOX67682.1"
                     /translation="MAEFARFHPRLLDTIKDYDRQRFISDLTAGITVGVIALPLAMAF
                     GIASGVKPEQGILTAIIGGFLVAALGGSNVQIGGPAGAFIVIVYGIVERYGLANLLIS
                     CALAGMLLFAMGALKLGGLVRYVPVSIVIGFTNGIAVLIALSQVKDFLGLSIDKMPSH
                     FFDQLEAIWQNIDSINPTALAIGAISLGGLFLWTRLFSPGGLLPVRLLEGRTVRGAAR
                     LPGSIVALVSLSIATALFSLPVETIGSRFGGIPTVLPSLALPTISWQSAQQLFIPTIT
                     IAILGAVESLLCARVADNLTPLPRHDPNQELMAQGIANMVVPLFGGIPVTGTIARTVT
                     NVRSGANSPVSGIVHAMTLLAIVLVAAPLAVHVPLAALAGILLFVAFNMGEWHEFRRL
                     RQFSAGYRTILIGTFLLTVIFDLTVAIEIGLVLSCVFFIYRMSTLFQVQPAAADEQPA
                     ATGVVLFNLYGALFFGAVTKVEGLVEKVPDGTRSMVLDLHRLVLMDTSGLDAMSQLHR
                     SLARKNVRLVLCRVNEQPLSLMRRAGFEAVLGAQNLRPDLQSAMAAAAEPA"
     gene            complement(23464..24225)
                     /locus_tag="PKV56_07425"
     CDS             complement(23464..24225)
                     /locus_tag="PKV56_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HOX67683.1"
                     /translation="MLLLTREWTAGDRTPNTNLRLGQVLAFVAGAANAGGFLAVGQYT
                     SHMTGIVSGMADNLVLGRIALVLSGGLALSCFLVGAMLTAILVNWGQQRNFHSCYALP
                     LVVEALLMLLFGLVGANLGLQTALLVPTTVLLLCFMMGLQNAIITKASRSEVRTTHVT
                     GMVTDLGIELGKLVYLNRRRAAAPNAPGVHANRARLRTHGSLVGLFLIGGVLGAAGFQ
                     QMGYSTTIPLALLLLLLSAGPLWRDLKQHRTVAHG"
     gene            complement(24242..>26319)
                     /gene="metG"
                     /locus_tag="PKV56_07430"
     CDS             complement(24242..>26319)
                     /gene="metG"
                     /locus_tag="PKV56_07430"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOX67684.1"
                     /translation="TTALPYANAPFHIGHMMEYIQADIWVRFQRMRGHEVHFVGADDA
                     HGAPIMIAAEKAGKTPQQFVAEIAAGRQQYLDGFHIAFDHWYSTDSPENTELAQGIYR
                     ALRDHADGSLITTRTIEQFYDPVKGMFLPDRYIKGECPKCGARDQYGDSCEVCSAVYA
                     PTELKNPYSTLSGAAPVMRSSEHYFFKLSDPRCVEFLEQWTGGERLQSEVGNKIREWF
                     TKDELGAGGLSDWDISRDSPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNYFDQGKAK
                     VRGEQRSFDEFIADPAVEQIHFIGKDITYFHTLFWPAMLQFSGRKVPDRVYAHGFITV
                     SGEKMSKSRGTGISPLKYLELGMNAEWLRYYIATKLSSKVEDVDFNPDDFVARVNSDL
                     VGKYINIASRAAGFLSKRFGGRLSGDVGVEGRVLLDHLRSGHAAVERLYDERDFGRAL
                     REIMLLADRVNEYVDQNKPWELAKQEGQDAVLQDVCTVCIEAFRLLTIYLKPVLPALT
                     AEVEAFLRIDPLVSTDAHRALGAHTIGEYRHLMQRVDPKLLDALFEPPAAAVVEHPKP
                     GGEDIAPVIGIDDFVKVDLRIAKIVNCEQVAGSDKLLRLTLDVGEVDAAGLPRTRNVF
                     SGIKSAYVPADLIGKLTVLVANLAPRKMKFGVSEGMVLAASHADEKGQPGIHVLEPWP
                     GATPGMRVR"
BASE COUNT         4055 a         8659 c         9042 g         4563 t
ORIGIN      
        1 ctacgacttc gccgccgaac tggccgacca gtacctggtg atggagcgcg gcgagatcat
       61 ccagcgcggt cgcggcaagg acatggaagc cgagggcgtg cgcgcccgca tgtccatcta
      121 gcgggttgcc acgatcaggg ctgaagcgcc gcaggcgatc tcgctgcagc gcttcgggtg
      181 caggttctgc ggccgcgctg atgtgccggg actaaccctt caacccggta cccagcacga
      241 agtcgaagtc ggccttcatg tatctgccgt cctgcttcat ggccagcgcg agtccgggct
      301 tgtggccgcc ccacgggtcg ccggcgagat gctcgtcgcc cttgaagtag agctgcgtgg
      361 tgagtgccgt gtgcaggaac ggttcgacct tgatgtgaat gtgccgcgcc cgggtcaggt
      421 tgccgtactt ctccagcccc ggaacgccgg gtggaatcgg gtacgcaccg ggcacgatgg
      481 tctcgatctc gtagcggccc ttcgcgtcgg tcaggaccat gccgcgcaga tgcggcttga
      541 ctgactccag gaaattgccg ggctgctgct tgtcgtacac gcccttgtcg tccgcctgcc
      601 agatctcgat cagcgtgtgg gccaggggcg tcctgcagtc cgcactgagc acggagccgc
      661 gcagcatcag cttctgtccg ggctcgtcgt cggcggccag cttgagttga accatcggtg
      721 cgccgaagtt gtacatgggc ccgagaattt cctgctcggt gagtcggcat tcggctgccg
      781 tggcttggag cgttggcagg cccgcggccg cgccgccgag tgttgctccg gcgtagcgca
      841 ggaaacggcg acgttgaatc gcgtggatct gctcttccag ttttcccatg tctgctccgg
      901 tcggtgctgt tgaattttcg agagcaggat tgtccaccgc gcatgcaccc aggtgcccgc
      961 ctggcctgaa tgcgagcaac ggcttgcgcc gattcaccga aaattcgctg cggacctacc
     1021 gctcgcgatc ccgctcaggt ccgccagacc cgcggaacgc agggctgcag gccccagccg
     1081 atttcgcgcc agcgcgccca gacgccagcc agcagttgga ttgccggctc gacacgtggc
     1141 gccagcaccc gcagcacgac gacctccgca tgtggcgaag tgcacccggc cgtgcgtgcc
     1201 agggcgtggg cgccagccag ttcacgcgcc gcatcgagca gcgtgtcgcg acgatcgggg
     1261 gccatcggcg agcctgccgc gaaccacagc gttgcgagca cgcgatggcc cgcgaagccc
     1321 agcggcgaat cgagcagccg cttgtcgtca ccgcggatcg tgccgcgctc gagccagacg
     1381 ccgggcaact cgatgcgctg catgtagtgt cccgagtcga agctgtcgtt gctggccggc
     1441 agacccagcg cgatgacgtc ccagcccatc atctcgccgc cgggtgacag ctcgaaacgc
     1501 atgctgttct cggccagtgc ggcgctgtgg cagatggtct ccagcggcag ccattcgagt
     1561 cgcgctccgt cctcgacgcg tgcggtcacg gactgccgcg ccgcctcgcc tgcactgcgg
     1621 tagaagcgcg tcgcgcccgg agtcgtgatc agtgcgtggg catgcggcgc cagcgtggcc
     1681 tcgacgtgaa gcacgtcgcc gccgacgatg ccgcccggtg gatgcaccag cacgtggtgg
     1741 cagatgcccg ggccttccgg atacaggctc tgcagcaccc gcagcgggcc cgagtggcgg
     1801 tcgtgcgcca gcgtgcgatc ttcggcgcgg cgatagttca gttcaagacg gcccggccaa
     1861 cccatggaga gcgcagtgtc gctcaagccg acttgacgcc gagcttgaac gcgatcatcg
     1921 cgcgcagctt gttcggcacc accggcttga tcagcaggtg gaaatccttc tgctgcgcct
     1981 cgaggtcgag cgccgacatg gtgctgccgg tgatgacgat ggccggcacg tgttcgccga
     2041 agcgggcgcg aagcaccgcg atggcgtcga cgccgttggc cccctgctcg agccggtagt
     2101 cgacgatcag caagtcgggc ttcacgagct gggtgtctgc gccgcgggcc catgccgcac
     2161 aggcggcgac gctggtgaag gccgcgatgc cggcccccca gccgctgagc agggcctcga
     2221 gtgcggagag caccgcgggt tcgtcctcga ccacaacgat cagccgaccg tcgagcgtga
     2281 tgccgagcgg gcccttgacc gacagcatcg gctgcgcttg aggcatccgc ttgcccggcg
     2341 gcaattcgag tgtgaagacc gtgcccttgc caggttgcga actgagcgcg accggcgcgg
     2401 cgatcaggtc ggccagccgc ttgacgatcg caagccccag gcccaggccc ttgcgctgat
     2461 gcgggtcggt gcgtggaccg ttcggtacct ggtagaactc ctcgaacacc ttggcctgct
     2521 cgccttcggc aatgccgggg ccggtgtccc acacctgcag catcaggcga tcaccgcgtc
     2581 gcctgcagct caccagcacg ctgccgtcta cggtgtagcg gatcgcgttc gagaccaggt
     2641 tgcgcatgat gcgttcgacc agcagcggat cggcgagcgc cacgtgctgc gagccgcgaa
     2701 agcgcagggc caggcccttc tcgaaggcca gcggctcgaa gtgcaggcgc agcttgcggt
     2761 acaggtcctt gatggcgaag tgctgcgggt ggacctcgac cccgccgctg tcgatgcgcg
     2821 tgatgtccag caactccgag aacaggcctt ccagcgcgtc gaccgattcg ttgatgctgt
     2881 tcaccagatg cgccacctcg acatcgtggt tcttctggcg cagcgcctcg gcaaacaggc
     2941 ccatcgcatg cagcggctga cgcagatcgt ggctcgctgc ggtgaagaac tgggtcttgg
     3001 cccggttggc gatttccgcc tgctggcgcg aggtgtcggc ggctgtcttt tccaaacgca
     3061 gttgcgaaag caggtgatcg gcctggagct tcagcgcgag cgcgcgttgc agcgcctggc
     3121 ggtagttgcg tgccagcagc gtggtcagcg agaagatcag cagcaggatg cctgcgagtc
     3181 gaaagctctc ttcgctgccg tcgctggcga cgcgtgccgc catcggaacg aagcacagcg
     3241 cggagaacag caggaacacc cgcggctgcg tcgccagcac cggtacgacc gcgatgcaga
     3301 acgtgtagat ggtgatgatc agtgcgatct gctgctcgtt gccgccgagc aggtagaaca
     3361 cccacgaggt cgcgccccac agggctcccg aagtcagcgt gcccaggttc caccacagcc
     3421 gccagcgcgc ccagtccacc acgccgaggg cagccactcg attgaaccgc tgcaacagcg
     3481 acagccgcgc aagccacagt accgcgaaca gccccagcca aggcagcatc accgacgcgg
     3541 gtgtgaccgc ccagaagagc agcacgatga cgccgatgcc ggccaggttg ccggccagct
     3601 gtgccggcat gtagtcgaag gcgctgcgca cctcctcggg cagcgaggct tcgggtgcct
     3661 cgccagccgc agccatcggc tcggcggtca tggaccgctc tttctccagc tgtgaaggcc
     3721 gccttgctgg cgcgacatct gccccactgc gagcacggcc tgggtgcgcg aactcacgtt
     3781 gagcgtgcgc agcaccgcgg ccacatggtc cttgacggtt tcaacggaca gcttgagatc
     3841 gcgcgcgatc agcttgttgg gcttgccctg cagcagcagg cccagcacct cggtctgacg
     3901 aggcgtcaag cccagcgatt cgagcggcgc atgtgactgg tagccttcgt ccgccgcgct
     3961 ctgtcgcacc gagcgcaggg cgcccggggt gctgtcggag tagaggtcgg cacctgccga
     4021 ctcgtcgccc atgttcatcg gcggtacata gatgccgccc gacatcacca tgctgagcgc
     4081 ctcgaacagc acgtcgttcg acgaacgctt cggcacgaag cccatggcgc ccaggtcgat
     4141 cgcgcggatc acgtcgctgg tgcggtcgct ggccgacacc acgaccactg gcatggccgg
     4201 ataggccgcg cggaattcgg tcagtacgtc gaagccatcg gcgtcgccaa gtcgcaggtc
     4261 gagcagtgcc agatcgaagt cgctgttctg cttcagcgtg tcgcgggcgg ccttcgcgct
     4321 gccggccccc acgacgctga cgttctcgcc caggccctgg atgatggtct gcagagcaga
     4381 caggatcagc gggtggtcgt cgatcagcaa gaccttcatg gtgtctcctc gggagtcagc
     4441 gccgcaggaa tgctaaggcc gcccacgcgt gacgtgccgg tttggaagtc acccccaaac
     4501 gggggtttgc ctgcctgcgc agggtgagga tcaggcgttc agccacgcgg tcagaggctg
     4561 ccactgctcg aggtcgcgct cgacacggct cggcgcgacg tcccacagcg tcagcccctg
     4621 cgctgcaagg tggatgtagt tctgcgtgtc gcgcagatgc gtcagcaccg ggaactcctg
     4681 cccgccgagg aatgccttca gctgatcggc agagatggtg ccttcgcgcg aacgcatgcc
     4741 gacgatgccg acctccagct tctgggcgcg cttgtgctcg gcgaggcggt gcaggaaatc
     4801 gtgtgttgcg cggatgtcga agatgctggg ctgcagcggt accagcactt tcgtggcgat
     4861 cttgaggatc tcgtcgagcc ttttgccatg caggccggcg ggcgtgtcca gcacgacgtg
     4921 ggtggtgccc ttgggcggtc gcacacgcag atccgcaccg gtgtcccagg cacggatcga
     4981 cgcggctgtc tccggccgct gtgccagcca ggaggccgcc gactgttgtc ggtcgacgtc
     5041 gccgagcatc accgctttgc gctgtgccgc gaagtatccg gccacgttgg tggacagcgt
     5101 gctcttgccc acgccgccct tcggattggc caccacgatc accggcatgc tcgacccctg
     5161 actccctgat gggctcaata ttagccgcgc ggtgggcgca gtcgcggggc agcgcgctca
     5221 gtgcggctgt gtccttcctg ccgctggagg gcggcgctgt ggctcgacac gacgtgcccg
     5281 gattcgttcg gctcggcggg tctgccggcc tggccctcga cgcagtcgct gctttgcgaa
     5341 cagcgggtga ccctttgcgc agcacctggc gacgcggaga ccctgacccg agcggggccg
     5401 gactctaatg ccaatgcaat tgcattagca tcgcgggatg gaacgtcaca cccggcaacg
     5461 tcaggccgtg ctcgatgcgc tggtgcgcag cggtcaggcg ctgagcccgc aggacctgct
     5521 gggacaggcg aaatcggccg tgcccagcct gaacctgtcg accgtctacc gccagatcaa
     5581 ggggttgcag gacgccggtc agatcgtccg cgtcgagctg ccggggcagc cgccgcggtt
     5641 cgagatggcc tcgcgcggcg aggcgactgc gccccatcgg catgcggccc atctcgttgc
     5701 ggatcagcgc cacgcgccgg acgaggcgtc ccggcatcac caccacttcc actgtgtcca
     5761 gtgcgaccgc gtgtatccga tccacgcctg tccgggtccg atgaacgatc tggccccgcc
     5821 cggcttcgag gtccagcacc acgacctgac cctgagcggt cgctgcgcga gttgcgtggc
     5881 gggagcgcct gcatgaaggc cggaaacatg gatgcggctt tgcacgtgca cgccgcgggg
     5941 cacggccatg gccatgcgca tggccacgac cactcgcacg atgacggtca cgatcatgcg
     6001 gcgcatgccg ccggctcctc cctgctgata ccgaagcccg caccagcgcc ggtttcgagt
     6061 ctgctgatgg cttcggcgct gctacggttc gccggagcca tcgggctcat cgcgctgctg
     6121 tggatggcgg tggtctgggc aatgtcggga acggcatgaa cggcatcgac gtcccggctc
     6181 tcccgaactc cacccagctt ccggtggtgg ctccacccgc catcgccgtc gaggacctga
     6241 ccgtcagcta ccgccaccac ccggcagtcc accatgtgag ctgtcgtttc gagcccggct
     6301 cgctcactgc ggtgatcgga cccaacggtg ccggcaagag ttcgctgctc gacgcgctga
     6361 cgggtcgggt gcaaccgagc accggctgtg tgcgcaccgg tggtctgccg cgcaaccggc
     6421 tggcctacat gccgcagcaa tcgggcatcg accgcacgtt cccgctcggc gtgctcgatg
     6481 tcgtgatgct cggtgcgtgg ccgcgcatcg ggtggttcac cggcgcgacc acggcacagc
     6541 gggcgcaggc gctgctggcg ctggcctcgg tgggcctggc cggtttcgag cagaggctga
     6601 tctcggagct gtcggtcgga cagttccagc gcgtgctgtt cgcgcgggtc ctgatgcagg
     6661 acgcgccggt gatcctgctc gacgaaccgt tcaatgcgat cgacacgcgc accacggccg
     6721 atctgatgcg gctggtgcag cactggcacg acgagggccg caccgtgatc gcggtgctgc
     6781 acgatctggc gcaggtgcgc gagcatttcc cgctgaccct gctgctggct cgcgagtgcg
     6841 tggcgcacgg gccgaccgaa gaggtgctcg tgccccgcca cctgatgcgc gcacagcaga
     6901 tggccgagca gtgggacgag aacgccgatt ggtgcgcagc ccctgtgctg gtgtcgaggg
     6961 cgccgtcatg acgctgctga tgtggttgca gggcatgctg atacagccct tcgtcgaatt
     7021 cggattcatg cgccgtgcgc tggtcgcctg cctggcactg ggactgagct gcgggccgat
     7081 cggcaccttg ctggtcttgc gtcgcatgag tctggtcggc gacgcgatcg cgcatgcgct
     7141 gctgcccggc gccgcgctgg ccttcgtgct tttcggcttc tcgctgtggg cgatgagcat
     7201 cggcggattc gtcgcggccg tcgcggtcgc aggcctggcg gggctggtcg cacgtctgac
     7261 gccccagcgc gaggatgcca gcttcgcggc gttctacctg atcgcgctgg ccgcgggcgt
     7321 gatgatcatc tcgatacgcg gcaacagcgt cgacctgatg cgcgtcctgt tcggcaccgt
     7381 gctggccgtc gacgacgcgt cgctgctgct gatcgccggc atcgcgagcc tgagcctgct
     7441 ggtgctggcg ctggtgtacc ggccactggt ggtcgagtgc ttcgaccccg ggttcctgcg
     7501 ccacctgggc aatgccgggg cctgggtgca cgcgctgttc ctgatgctgc tggtgctcaa
     7561 tctcgtgggc ggcttccagg cgctgggtac gctgatggcg gtcggtctga tgatgctgcc
     7621 cgccaccgcc gcgcgcttct gggccgccga ggtctggagc ctggctgcgg tggccgctct
     7681 gttcgccgtg ctcagcggct ggggcgggtt gctgctgtcg tatcacgcca acttgccgtc
     7741 gggcccgtcc atcgtgctgc tggctggcag tttctacgcg ctgtctctgc tgatcggccg
     7801 gcgtgacagc ctgcttgcac ggcagttgtc gcgccgctca caccgcaagg cctgatgatg
     7861 caatttgatt cgacaccgcg cgcgctgagg cgtgccttcc tgactttgct cgcatccgcg
     7921 ggaacggccc gctggtcggt ggcaccggcc gcgcagactc cacccccggt gctgaaggtc
     7981 gtcgccacct tttcggtgct ggccgacatc gcacgcgagg tcggtggcac ggccgtcgag
     8041 gtgactgcgc tggtcgggcc gaatgccgac gcccatgtgt tcgagccgac gccgtccgat
     8101 gtgcgcaggg tggcgagcgc cgatgtggtg gtaaccaacg gcctgcgtta tgaaggctgg
     8161 atcgaccgtc tgatcaaggc gtccggtttc cggggtccgg tcgttgtcgc cagccagggg
     8221 gtagtgactc gcggggtgtc cggcggcgct gatccgcacg cctggcagag tctcgccaac
     8281 ggtcagcgtt atgccgagaa cattcgtgcg gtgctggtgg cggctttgtc ggccagggca
     8341 cccgcgcgcg tggcggaggt cgaggcgcgt gctgccgcct accagcagcg cctggctgcg
     8401 cttgatcgcg atacccgcga gcgcttcgcc gcgctgcccg ccgagcgccg gcgcgtgatc
     8461 accgcgcacg atgcgttcgg ctatttttcc gacgcctacg gggtcgtgtt catcgctcct
     8521 cgtggctgga ccaccggcag cgagccgtcg gcaaaggcgg tggcggccat cgtccgtcag
     8581 gcccgtgcat cgaaggccag tgcgctgctc gtcgagaaca tcagcgaccc caggctgatc
     8641 gagcgcatcg gccgcgaatc cggactgaag gtcggcggcg cgctctattc cgacgcgctg
     8701 tcgccggtgg gtaccgaagc cgatacctat gtgcgcatgt tcgagcacaa cgtgaagaca
     8761 ctggtggacg ccatgtcgct gaacgttcct ggagcgtcag gcgatgccgg cgccgtgcct
     8821 cgctagttcg cagcggatac ggtcggcatg aattcccgcg ctttcaacag cacctggctt
     8881 cgcgaggaac tgctgcacct gctctccctg tcgggcacgc tggccggttt ctccgtcacg
     8941 gccgtcacgc tgctgcgtac gatcgggcag ccctcgctga tgcagaccgt ggccgatgac
     9001 gtgctggcga tgtcggcgct gcttttcctg gtctgcacct atgcgatctt cattgcactc
     9061 aggacgcagc gccaggcgct cgctcgcgcc ctggagaagc tcatcgacgg cctgttcgtg
     9121 ctcgcgctca ccggcatggt ttccgccgga ttcatcatgg tgtacacgtt gtggtgaact
     9181 gcagggaaca agatcgaaag tccttccagg caaggtcttc ggcccggatt ttctgcaaaa
     9241 cctgtttcgc tgggggcctg acgatcagtc tgaacactag gcctttcgcc atgccgctct
     9301 cgaccgcggc atgatttcgc tcatctcctg aaacccccgc tcaccgccat gtccgattcc
     9361 aaccccgtca gcaccgaatc cctctcagtc ctcggcatca agaccggtcg cgccgggccg
     9421 cggctgctgg tactgggcgc cgtgcatggc aacgaaacct gcggcccccg cgccatcgag
     9481 aaggtgctgg ccgacttcga tgtcgagaag ctcaagctgc tgcgcggcaa tgtcaccttc
     9541 gtgccgatca ccaacccgct ggcctaccag cgaggcaggc gagaaggcga tcgcaacctg
     9601 aatcgcaatc ttcgaccgac cgacacgccg caggatttcg aggaccgcat cgccaacgcg
     9661 ctgtgcccgc tgatcgcgca gcacgacatg ctgctcgacc tgcactcgtt ccagtcgccg
     9721 ggcgagccct tcgcgatgct cggcccgctg aacaacgagg acacgctgca gccgttcgaa
     9781 ctggctgcgc acgaggaagc ggtggcggtg cgcctgggcc cgaagcgctt cgtcgaaggc
     9841 tggctcgaga cgtatgcggc cggtgtcgcc aaccgcgtca agcgggccga tcaatcggtc
     9901 gatccgaatg cgcgcgccac cttgctcaac accgatccga gctacggcat cggcaccacc
     9961 gagtacatgc gcagcgtcgg cggtgtggcg atcacgctcg agtgcggcca gcacgacgat
    10021 ccgaatgcgc cggaagtcgg ctatcgcgcg atcctcaacg cgatggcaca cctggcgatg
    10081 atcgaagcgc ctcgcccgga accgcgcacc gatgtcgaag tgttgcgact gaccgaggtc
    10141 accgatcgcc tgcatgcgga cgatgccttc gtgaagccgt gggccagctt cgatccggtg
    10201 tcggccggcg agcccatcgg ccgccgtcac gatggaacgc tggtcaccgc gccgagcgat
    10261 gggttcatcg tcttcccgaa caccaaggcc acgccaggca acgagtggtt ctatttcgcc
    10321 cggaccagcg atcggaaact gaacgtcgag tgaggattgg cgtcgcgtgc cgcggcgtgg
    10381 ctggcgcggg ccgacctttt cgctgccgaa agagaaacca tgaaacgtat ctccgcctgg
    10441 gtcgccgcac ttttcctgtc caccggcacg gtggtgctgg cgcagggcca gccgcatgtc
    10501 cacggcgtcg ccaggctcga tgtcgcagtc gaggccgggc aggtcgtgct gcagttcgag
    10561 acgccgctcg acagcctgct gggcttcgag cgggcgccgc ggggcgcgga cgaaacgcgg
    10621 cgggcggagc tggccatcgc tgcactgaag gcggccgatg cgctgttcag gttcacgccg
    10681 gccgccggct gcacgccggc ccgtgtcgat ctcagatcgg cggccctgaa gctgggctcg
    10741 cccgacccgg ccgaggcgca cgaaggccac gccgacctcg atgccaccta cgtcttcaag
    10801 tgtgttgact cggccaaggc agcggaggtc gatgtcgggc tgttcgaagc gttcagtcgg
    10861 ctgcagggcc tgagggtcga ggtcgtgacg gcgcgcggcc agttcaagcg cgacttgaag
    10921 cggcccgcga agcgcgtatc tctggctcgc tgacccattg cctcccgtgc aaagcccacg
    10981 tccttcctgc gccgccgcgc gctgcaacag tgagtgacgt cgcgtgatcg aggtgcgtga
    11041 tcttcgcttc gcctggccgg gcgccgcgag cgactgcgtg gtcatcgacg cgctgagcat
    11101 cccggccggc cgcacggtgt tcctgcacgg ccccagcggc ggaggaaaga gcacgctgct
    11161 cggcctgctg gcgggcgtgc tgttgccgcg cgtcgggtcg gtcagcctgc tcggcacccg
    11221 ctgggctgat ctgtcgggcg cgcgtcgcga tgcgttccgc gccgatcatg tcggctatat
    11281 cttccagcag ttcaacctgc tgccctacct gagcgcagtc gacaacgtgc tgctgccatg
    11341 ccgcttctcg cgtctgcgtc gcgaacgggc cgcgcaggac ggtggatctc ccggccacgc
    11401 cgcacgcgag ttgctcgagc gcgtcgggct ttcgcaggcc ttgtggacgc gagctgccgc
    11461 acagctgtcg gtcggccagc agcagcgcgt cgcggcggca cgtgccttga tcggtcggcc
    11521 cgaggtggtg atcgccgacg agccgacctc cgcactcgat gccttgctgc gcgacagctt
    11581 catggatctg ctgctcgatg cctgtcacgc atcgcgcggc acgctggtct tcgtgagcca
    11641 cgatgagcgg ctggcagcgc ggttcgacga gcgactctct ctctcggcgg tcaaccggac
    11701 agcgtcgtcg atgcaggccg aggattccct gtgagcgcat tgctgacgct ggcatggcgc
    11761 agttcctgga gccgacgtgc gacgctgtct ctggtgctgc tgtccgtggc gctttcgacg
    11821 ttcctgctgc tgggcttcga gcaggtgcga accgatgtcc gcgagaactt ctcgcagtcg
    11881 gtcagcggaa cggacctggt ggtcggcgcg cgcaccggat cggtccagct catgctgtat
    11941 gcggcgttcc acgtcggcag cgcgaccaac aacatccgca tggacagcct cgccgcgatc
    12001 gcgcagcacc gggccgtcga ttgggtggtg ccgctgtcgc tgggcgactc gcatcacggt
    12061 tttgcggtgc tgggcacgac gtccgattac ttcaaccgat tcctgtacgg cgaccgcgag
    12121 ccgctggtgc tggcgcaggg ccgcgccttc acgaacggca tcgacgggtt gtatggcgcg
    12181 gtgctcggcg cggaggtggc gcacgcacgg cgctacaggc tcggtgacaa gctgaccctg
    12241 acgcacggca gcggtggtct gcccggctcg gagcacgccg acaagccgtt caccgtggtg
    12301 ggcatcctcg cgcgaaccgg cacgccggtc gatcggacgg tgcacatcag cctcgaatcg
    12361 ctggaagcga tccacatcga ctgggtgggt ggcgcgcctc tgcctggcgt cgtgattgcg
    12421 cccgagcagg cgcgcaagtt cgatctggcg cccaagcagg tgaccgctgc gctggtcggg
    12481 ctgaaggggc gggcggcggt gttcaatgtg cagcgcttcg tttccgggta tgccggcgag
    12541 ccgctgatgg cggtgctgcc gggggtggcg ctcgacgagt tgtgggacgt ggtcggcttg
    12601 ggcgagaagg cgctgctcgg catgtcggcc atggtcggcg tggtcagcct ggcggggttg
    12661 attgcggtgg tgatggcagg cctgaacgag cggcgccggg aactggcggt tctgcgtgcg
    12721 gagggcgccg ggccgcgcca cgtgctgctg ctgctggcgg gcgagggcct gatggtcacg
    12781 ctggcgggtg cactgctggg tgcgctcgca acgggcctgt tcatcgcgac gagcgcgccc
    12841 tgggtgcagg ctcaccacgg agtcaacctg aaactttccg tgccgagcga gacccaatgg
    12901 atgctgctcg ccgccgtcgt gctcgcgggc ggcgtcgcca gtctgattcc ggcgtggcga
    12961 gcctaccgac tgtctctggc cgatggcctg tccccgaggg tgtgaagatg aagaatggat
    13021 cgtgggtctg gggcgttgcc gctgccacag ggcttcgcgc agtggcaacg atggtgggtg
    13081 tcctgctgct cggtgctgcg ggcagcagct gggcgcagga cggcggcgcg ggtgccgcgg
    13141 gatcgccttc gctgagccga ccgctcggcg gctcgggtgc gagcaaggcg gctgcgcccg
    13201 gcactttccg cgagatcaag tgggatgacc tggtgccgca ggactgggat ccgctgaagc
    13261 agttcaagag caccaatttc agcgtgatga acgacagcga tccgcgcgcc accgagatgc
    13321 tcaagcgcat gcgcgagacc tgggacaacg cgccgaccaa caacgagatg gatggcgtcg
    13381 cggtgcggat tccgggctac ctggtgccgc tggaagacaa caagagcggc ttgaaggaat
    13441 tcctgctggt gccgtatttc ggggcctgca tccactcgcc accgccgcct gccaaccaga
    13501 tcatccacgt caaaccgcag aagccgccga agggcttcca ttcgatggac acggtctggg
    13561 tcagcgggac gttgaagacg ctgcgcagcg acagctacat ggggtcgagc ggctaccgga
    13621 tggacgcggt gagcgtcgag gcctacgtcg agaagtgaag ggtcggctca ggcccgggcg
    13681 acccggtcgc ggccgccggt cttcgcctga tagagcgccg catcggcgcg cgcgatcagc
    13741 gatgacgcat cgtcgtcagg ccgcatctcg gcaaccccgc ccgagatggt gacggtcagc
    13801 agcacttcct gcgtggtgcg gtggcggatc ttcatggcct tgatgcgggt gcgcaccgtc
    13861 tcggcgagct ggatcgactg gtcgagcgag gtctgcggca gcagcagcgc gaactcctcg
    13921 ccgccatagc gagcggcggc atgcgtccca ttggtgaccg aggtgcgcag gatctctccc
    13981 acggcctgga tcacccggtc acccatcagg tggccgtgcg tgtcgttcac cttcttgaag
    14041 tgatcgatgt cgagcatcac caggcagtgg ctggttccgt cgatcggctt gttcgtgagc
    14101 agcgactcga tccgtttctc gaaaccccgt cgattgagga tgcccgtcat cggatcgagg
    14161 atcgcatcgt cgcgtgcgcg ttcgaggtcg ctgcgcaggc ggttgatttc gtcctggctg
    14221 gcggtgacct tctggcgcag cgcctcgacc gagctcttca tctcggcggt gccggccatc
    14281 acctcggtga ggcgcggctt caattgctcc acatcgcgcg atgccagtgc cgtgttcagc
    14341 cccgagagct ggtcaccgaa ggtgcccgcg cgctcgcccg tctgcgaagc cgtttgcacc
    14401 atgctggcca tcaggcgttc catctcgccg ctgatggtct tcatcgtctg ctcgtccgcc
    14461 ggagcgatgt gccgcttgta gagttcgacg acctcgtcgt catcgatgcc cgactcgtcc
    14521 ttgatcagcc gatcgaccga cgaagccagg cccgggttga tccccgccac gtactcgtac
    14581 caaagcgtga aggtgacggg attgaatgcg gcggcgtggc ggcccatgtg acccaaagcc
    14641 agacgcagca gttcggcggt tcgctctttg gattccgagt aacgcaaggg cactccaggc
    14701 aaaaaggtgg tttttataga cctgcggagt ttagtgtcaa taggtattcc atgccgcatt
    14761 cgcgagccgc tttcatggcg atcaggttcg gcccgcacgg ggcgtgatcg accggcccgg
    14821 gacagcgacc cggccgcacg acaatcgccg gatgttcgct ccctcgcaaa acgacgtgcg
    14881 ccggttcttc tgtgctgccc atcagaagtc gcgtgatggt ctgccgatga cggccctgga
    14941 aatcctcgct gccgactgga tcgccgagca ccccgagtac cacgccgaac tcgccgatgt
    15001 cgatgcggcg ctggccgccg tctacgacgt cggggccggg cgcagcaacc ccttcctgca
    15061 cctgtcgatg cacctgtcga tcagcgagca ggtgtcgatc gatcagcctc gtggcatcag
    15121 gcaggcggtc gagcttctca ccgcgcggcg cagttcggca cacgccgccc atcacgaggt
    15181 gatggaatgt ctcggggaca tgatgtgggc ctcgcagcgc agcggtctgc cgccggacgg
    15241 cgagcgctac atcgccagcg tcaggcagcg cgccacgcgg gattgacgac gaattcccgc
    15301 gggcgggcac ccgagggcga gacccgcagc tccggttcat cacggcttca gggagcgctt
    15361 ctaccgtgtc tggatggatg ccacgcaacc gacgacagtg cagcgcgatg cgatgtggac
    15421 gctggcgttt cttgctttcg tactggcctg ggatgccggc ggcggcgacc tccgggtcat
    15481 gcatctgttc ggagacgcgc acggcttcgc ctggcgcaac cactggctgc tcgtcggctg
    15541 gttccacgag ggcggacgct ggctgagcgc gctggtggtc ggggtcgttc tcgtcaacgt
    15601 cgtgcggccc tggggatttg cgcgagacat gtcgacgctg acccgatggt ggtggtgcgc
    15661 ggccacgttg acgtgcctgc tgctgatacc gacgctcaag cacggcagca tgacgagttg
    15721 cccctgggac ctgaccgagt tcggtggctc ggcgcactac gtgtcgcact gggcacggca
    15781 catcagggac ggtgggcccg gtggatgctt tccggccgga cacgcttctg ctgctttcag
    15841 cttcttcggc ggatggttcg cactgcgcgc cacgtcaccg aaggccgctc gacgctggct
    15901 cgccggcgtg tgggcctgcg gcatcgcgtt cggcgtggcc cagtcgctgc gaggggcaca
    15961 ttaccccagc cactcgttgt ggactgcctg gctgtgctgg accaccagcg ccctgatgtg
    16021 gcacgccagc agtcgcttgc gcatgatgcc gatgcgtcgt gtcgaggccg tggtgggcag
    16081 ttgatcccgg cggcaggact cagatgctga attcgaggtt gtcgatcaac cgggtcgcgc
    16141 ccagcctggc cgcgccgagg gcgacccagt tgccgcgccg atcatcgctt tccgcgggag
    16201 acagaaggtt gtcctgtcgg cgcaccgtca ggtagtccgg cttccagccg cgtgccgtca
    16261 atgcctgccg ggcctgcgat tcgatcagat cgacaggccc gataccgtca cggaagcgtt
    16321 ccgtcagcgc atgcagtgcc tgcgacagtt cgacggcctg cgcgcgctcg gcctggctca
    16381 ggtagccatt gcgagacgac agcgcgagcc cgtcgtgggc gcgcagggtc tcgccggctt
    16441 cgatcacgat cggcagcgcg aactggtgcg ccatccggcg gatcaccatc agctgctggt
    16501 agtccttctt gccgaacacg gcgacccggg gctgcactgc attgaacagc ttcaggacga
    16561 ccgtggcgac gccggtgaag aagccgggac ggaagtgtcc ctcgagaatg tcggccaggg
    16621 ccgggtctgg ctgcacggtg aaggtctgcg gctcggggta gagctcgtgt tcgtcgggcg
    16681 caaagacgat gttgcagccg gccggtgcca gcagctcgca atcgcgatcc agcgtgcgcg
    16741 ggtagcggtc gaagtcctcg tgcggtgcga actggaggcg gttgacgaag atgctcgtga
    16801 ccaccggcag gccgtgcgcc gcagcctgtc gaaccagtgc caggtgtccg tcgtgcaggt
    16861 tgcccatcgt cggcacgaac gtgcaggttc cggggactgg cagcgccgcg cgcaatgcgg
    16921 cgatgttgtg aacgatcagc atgctcaata cccgtgcagt gccgggtcgg gaaagcggcg
    16981 ggccttcacg tcggcgacga aggtggccac ggccgcctcg atcgacggtg cgcccttcat
    17041 gaagtcgcgc acgaagcggg gcagcctgcc ctgggtcacg cccagcatgt cgtgaagcac
    17101 cagcacctgg cccgagcagt cgacgccagc gccgatgccg atgacgggaa tgcccaccgt
    17161 ctgggtgagc gcgcggccga cctcggaagg caccagttcc acgaccatca tggctgcgcc
    17221 ggcctgtgcc agttcggtgg cctgacgcag cagcagcgca accgcttcgt cgctgcgccc
    17281 ctgcacgcgg tagccgccga gcgcgtgcac cgattgcggc gtcaagccga ggtgggcgca
    17341 caccgggatg ccgcggtcca ccaggaagcg cacggtctcg gccgtccagc cgccgccttc
    17401 gagcttgacc atgtgggcac ccgcctgcat cagcaccgtg gcactggcca gcgcctgctc
    17461 gcgcgattgc tggtagctgc cgaatggcag gtcgctgatc agccaggcgc tgcggttgcc
    17521 gcgggcgacg cagcgggtgt gatatgcggc atcctcgagc gccaccggca gcgtgctgga
    17581 tcgcccctga accaccatgc ccagtgaatc gcccaccatc aggcagtcga cgccggcgct
    17641 gtcgagcagg cgtgcgaagc tggcgtcgta gcaggtcagc acgctgatcg gctcaccggc
    17701 ggcatgcatc tcgcgcaggc gatggagcgt gaccggcttg cgaccggccg gcgcggcctc
    17761 gttggacggg tggctgctca tggtcgggcg ctcaggcggc cttcatgccc agcgtctcga
    17821 gcagcacctg atcagccgac acatcggggt tgccggtgac cagcagcttg tcgccgtaga
    17881 agatcgagtt ggccccggcc aggaagcaca gcgcctgtac cgcgtcgccg agcgcctggc
    17941 ggccggccga cagccgcacg cgggtgtgcg gcatcgtgat gcgtgcggca gcgatcgtgc
    18001 gcacgaactc gaacggatcg agcggcgcct gcccgtgcag cggggtgccc tcgaccggca
    18061 ccaggtggtt gatcggcacc gattcggggt agggctccat gttggccagt tcggcgatga
    18121 gcgcagcgcg ttgcgtgcgt gtctcgccca tgccgacgat gccgccgcag cagaccttga
    18181 cgcccgcgcc gcgcacgcgc tgcagcgtgt cgatgcggtc ctcgaactgg cgcgtctgca
    18241 cgatgtcggc gtacttgtcc ggcgcggtgt cgatgttgtg gttgtagtag tcgaggccgg
    18301 catcgcgcag ggcttcagcg tggccgtcgc ccagcatgcc gagcgtcgcg caggtttcca
    18361 tgcccaggcc cttcaccgcg ctgatcagct gtgacacctt ctcgatgtcg cgatccttcg
    18421 ggccgcgcca ggccgcgccc atgcagaagc gcgtggcgcc ggcggccttc gcctggcgtg
    18481 cggcagccag cacctcgtcg accttcatca gcgcggtcgc ctcgacgccg gtgtcgtggg
    18541 cggccgactg cgggcagtaa gagcagttct cggcgcagcc tcccgtcttg atcgacagca
    18601 gggtcgacag ctgcacctcg gtcggatcga agttggcacg gtgcaccgtc tgcgcccgga
    18661 acagcaactc ggggaagggc agcgcgaaca gcgcctcgat ctgcgccacc tgccagcgtt
    18721 gcgtgccggc accggaggcc gcggcgggag cggtgtttcg aacgagctgg atcggggctt
    18781 cttgagggga tgttgcttgc atttttcggg tctcgcttgg gcgctgccgc aatgatgcgc
    18841 cggatcgttg gggaatgtgg gtaggccgtc aaacggcctg gtaggccagg cgcacgtaga
    18901 tcggcgcgta ggcttcggcc tgcgtgatct cgagcagcat ctcgcgcgcc agttccagca
    18961 tcgcgatgaa cgtgacgacc agcaccggct ggccacgcga cacgtcgaac agttcgtgga
    19021 attcgacgaa cttgcggccc tgcagcgcgc gcagcacgat gctcatgtgc tcgcgcacgc
    19081 tgagctgctc gcgattgatg gtgtggtgct gcgtcaggtt cgcacgcttg agcacgtcgg
    19141 cccaggccgc gcgcagatcg tcggggtcga catcggggaa acgtggcacc agcgactgct
    19201 cgatatgcac ctgtgcacgc aggaagtcgc ggcccagcac cggcagcgcg tcgaggcgtg
    19261 cggcagccag cttcatctgc tcgtactcga ccaggcggcg aaccagctcc gcacgcgggt
    19321 cttccggctc ggcgccgtcg gggctgcgct tgggaggcag cagcatgcgc gacttgatct
    19381 cgatcagcat cgctgccatc agcaggtatt cgctggcgag ttcaaggttg gtcttgcgga
    19441 tctgctcgac gtaggtcagg tactggcgtg tcacgtcggc cagcgggatg tcgaggatgt
    19501 tgaagttctg ccgcctgatc agatacagca gcaggtccag cggcccctcg aaggcctcga
    19561 ggaacagctg cagcgcatcc ggcgggatgt acaggtcgtg cggcagcttg aacagcggct
    19621 cgccatagag ccgcgccact gccacgccgt ccagtaccac cggcgggatg ttcgccacgt
    19681 cgtccgccat gtcctgcgaa gtcagtgcct gcagctcggg gggctccagc agcgccgggc
    19741 cggggaggtt catcgcccgg ccggcttact tcggcccggt ctggtaaacg tagggccgct
    19801 gtggcacgcg cgactcggcg tcctcggcct gcgcgtcacg gtcgacctgc ttgtcccaca
    19861 gcagtgcgcg cccggcgcgc tgctcggctt cgagcgaggg cttcttcgcc ttcagggttt
    19921 cgatgaaatc ggtcacgtcc gacttgtagg gtttccagaa cagcggcatg gtcaggtggg
    19981 gctcggcgtc cggcaggcac gtcgatggcg agtggcacag aggaaacccg gagtttaccg
    20041 gggcaggcga gtcgggccac gcgcagaccg gcgccgcaac ccggcctttc cctgaaaccg
    20101 gtttcatcaa atttgcgggt tgacctgtca cgatgccgcg atgcaggaag tcgagctgaa
    20161 gtttcaggtg ccgccacagg cgcgcaaagc ggtcgaatcg gcagtcgccg ggccgcatcc
    20221 cgctcgccgc atccgcttgc aggccgccta tttcgacacg cccctggggg ctttggccgg
    20281 ggctgcgctg gccttgcggc tgcgcaagga gggccgggtc tgggtgcaga cgctcaaggg
    20341 caccctgccc gacggcaccg gcatgatccg ctccgaacac aacgtgccgc gggtcgaagc
    20401 cggtcccgac gcgccggcca tcgacccgca cctgcacgcc gggacccctg tcggcgcagc
    20461 gctgctgaag gtgctgcagc aggccgatgg cgaactgcgc agcgtgatgg tcacggacat
    20521 ctggcggcgc gtgcgcacca cgcgggtacc cggcggatgg gtcgaactgg cgttcgacgt
    20581 cggcacgatc gaggccggac cggccgaagc gccccgccgg gtgtccgtgt gcgaactcga
    20641 gatcgagctc aagcgcgggc acccgcaggc ggtcgtcggc gcagcgcagc gctgggtcac
    20701 gcgccatggg ctgtggctgg acacccgcag caaggccgaa ctgggcaacc tcctgcttcg
    20761 ccacgtggcc atggcggctc cgcgccgtgc cggcgaggtg atcctggcaa ggtcgatgtc
    20821 gcccgcgcag gcgctcgatg tggttctgcg ttcgtgcctg gagcagatca gcgtcaacgc
    20881 cagccagatc gccagcggcg tgcacgcgcc cgaacacgtc catcagttga gggtcggcct
    20941 gcgccgattg cgcaccgcgt tgcggttctt cggtggctca cagctggccg aggccatcga
    21001 cgcggggacg cgtgacgcgc tggcggccga tgccgcggaa tggtttcgtc tgctcggaca
    21061 ggcgcgcgac cgagatgcag tggccggccc gctggcgatc gatctggccc gcgcgctctc
    21121 cgcggtgggt ctgtcgggcg agatgcctgc cttgcccgcg accggcgacg agatcgaccc
    21181 gaccctggtc gtgcgccgtg gcagctccca gcgcctgatg ctgggactga tctcgcacct
    21241 ccagcgcggc cagccgacgg tcgcaggtgc tgcagtgcag gttgcgtccg cggcagcgca
    21301 gccggtggtg tcggagcccc ggatcgagga ccccatgacc tcactgactc tgcgcaagcg
    21361 gctggttcag cggctgaatc gctggcatcg gcaggtcgcg gccgacgcgc agcgcttcga
    21421 cgccctcgac gatgagggcc ggcaccggct gcgcaagcat gtcaagaggt tgcgctatgc
    21481 ggtggaattc tccgctggcg tgttcgatgc gcgtgaggtg cgacgctacc tgcagggact
    21541 gcgcgcgttg caggagcgtc tgggcatcgt taatgatgtg gcggtgggca tcgcgctcta
    21601 ttcggctgcc gcgcccgccg atgcgcgcgc tctgtttgcg ctgggctggc tcgcctcgca
    21661 gcgcgaccgg gctctgcagg catgccggcc ggaactgaag cgcttcctcg ccatgccccg
    21721 gttctggcgt tcgcgcagga acaagaccgc ctcgaagtga aaggttctgt cctggttgcg
    21781 gagccggctc gtcaagccgg ctccgcagca gctgccatcg cggactgcag atcggggcgc
    21841 aggttctgcg cgccgagcac cgcctcgaat ccggcccgcc tcatcagcga cagcggttgt
    21901 tcgttgacac ggcacagcac caggcgcacg ttcttgcgtg ccagcgaccg gtgcagctga
    21961 gacatggcgt cgagccccga ggtgtccatc agcaccagac ggtgcaggtc cagcaccatc
    22021 gaacgggtgc cgtcgggaac cttctcgacc agcccctcga ccttggtcac cgcgccgaag
    22081 aacagcgcgc cgtagaggtt gaacaacacg acccccgtgg ccgccggctg ctcatcggct
    22141 gcggcgggct gcacctggaa cagcgtgctc atccggtaga tgaagaagac gcaggacagc
    22201 accagtccga tctcgatggc cacggtgagg tcgaagatga ccgtcagcag gaaggtgccg
    22261 atcaggatcg ttctgtagcc ggcgctgaac tggcgcagcc gccggaactc atgccattcg
    22321 cccatgttga atgcgacgaa cagcaggatg ccggccagcg cggccagcgg cacgtgcacc
    22381 gccagcggtg cggcgaccag aacgatcgcg agcagcgtca tcgcatggac gatgcccgac
    22441 accgggctgt tcgcgcccga gcgcacattg gtgacggtgc gcgcgatcgt gcccgtgacc
    22501 gggatgccgc cgaacagcgg caccaccatg ttggcaatgc cctgtgccat cagctcctgg
    22561 ttcggatcgt gacgcggcag cggcgtgagg ttgtcggcga cacgcgcgca cagcagcgat
    22621 tcgaccgcgc cgaggatcgc gatggtgatc gtcgggatga acagctgctg ggcgctttgc
    22681 cagctgatgg tcggcagcgc gaggctcggc agcacggtcg gtatgccgcc gaagcgcgaa
    22741 ccgatggttt cgaccggcag gctgaacagc gcggtggcga tgctcagcga aacgagcgcg
    22801 acgatcgaac ctggcaatcg ggccgcaccg cggaccgtgc gtccctcgag caggcgcacc
    22861 ggcagcaatc cgcccggact gaacagcctc gtccacagga acagcccgcc caggctgatg
    22921 gcgccgatgg ccagggcggt cgggttgatg ctgtcgatgt tctgccagat cgcttccagc
    22981 tggtcgaaga agtgcgatgg catcttgtcg atcgacagcc cgaggaagtc cttcacctgc
    23041 gacagtgcga tcagcaccgc gatgccgttg gtgaagccga tgacgatgct caccgggacg
    23101 tagcgcacca ggccgcccag cttgagcgcc cccatggcaa acagcagcat gccggccagc
    23161 gcgcaggaaa tcagcaggtt ggccaggccg taacgctcga cgatgccgta gacgatgacg
    23221 atgaacgcgc cggccggccc cccgatctgg acgttcgatc cgccaagcgc cgcgaccagg
    23281 aagccgccga tgatcgctgt caggatgccc tgttcgggct tgacgccact ggcgatgccg
    23341 aacgccattg ccagtggcag cgcgatcacc ccgacggtga tgccggcggt caggtcgctg
    23401 atgaaccgct ggcggtcgta gtccttgatc gtgtcgagca gccgcggatg gaagcgtgcg
    23461 aattcagcca tgagcgaccg ttcgatgctg cttcaggtcg cgccacaggg gccctgcgct
    23521 gagcagcaac agcagcaggg ccagcgggat cgtcgtcgag taccccatct gctggaaacc
    23581 ggcggccccg agcacgccgc cgatgaggaa cagtccgacc agtgaaccgt gcgtccgaag
    23641 gcgggcccgg ttggcatgca cccccggggc gttgggcgcg gccgctcggc gccggttcag
    23701 gtataccagc ttgcccagtt cgatgccgag gtctgtgacc atgccggtca cgtgcgtggt
    23761 gcggacttcc gaacgtgacg ccttcgtgat gatggcgttc tgcaggccca tcatgaagca
    23821 cagcagaagc accgtggtcg gcacgagcag cgcggtttgc agtcccaggt tcgcgccgac
    23881 caggccgaac agcagcatca gcaaggcttc gacgaccagc ggcagggcgt agcagctgtg
    23941 gaagttccgc tgctgtcccc agttcacgag gatggcggtc agcatcgcac cgaccaggaa
    24001 gcagctcagc gccagcccgc ccgacagcac cagggcgatg cgcccgagca ccaggttgtc
    24061 agccatgccg gacacgatgc cggtcatgtg cgacgtgtac tgccccacgg ccaggaagcc
    24121 cccggcgttg gccgcgccgg ccacgaaggc aagcacctga cccaggcgca ggttggtatt
    24181 cggcgttcga tcgcccgctg tccattcccg cgtcaggagc agcatggtct ggcgccgggc
    24241 gtcagcgcac tcgcatgccc ggcgtcgcac ccggccaagg ttcgagcacg tggatgccgg
    24301 gctggccctt ctcgtcggcg tggctggctg ccagcaccat gccctcgctg acgccgaact
    24361 tcatcttgcg cggtgcgagg ttggccacca ggacggtcag cttgccgatc agatcggccg
    24421 gcacgtaggc gctcttgatt ccgctgaaga cgttgcgcgt gcgcggcagg ccggctgcat
    24481 cgacctcgcc gacatcgagc gtcaggcgca gcagcttgtc ggagccggcg acctgttcgc
    24541 agttgacgat cttcgcgatg cgcagatcga ctttcacgaa gtcatcgatg ccgatgaccg
    24601 gcgcgatgtc ctcgccgccg ggcttgggat gctcgacgac ggccgccgca ggaggctcga
    24661 acagtgcatc gagcagcttc ggatcgacgc gctgcatcag gtgccggtac tcgccgatcg
    24721 tgtgggcgcc gagcgcgcgg tgggcgtcgg tcgataccag cggatcgatg cgcaggaacg
    24781 cctcgacctc ggccgtgagc gccggcagca ccggcttcag gtagatcgtg agcaaccgga
    24841 aggcctcgat gcagacggtg cacacgtcct gcagcaccgc gtcctggccc tcctgcttgg
    24901 ccagttccca gggcttgttc tggtcgacat actcgttgac gcggtcggcc agcagcatga
    24961 tctcgcgcag cgcacgaccg aagtcgcgct cgtcgtagag ccgttcgaca gcggcgtgac
    25021 cgctgcgcag gtgatcgagc agcacgcggc cttcgacccc cacgtcgccc gacagccggc
    25081 cgccgaagcg tttgctcagg aagcccgccg cacggctcgc gatgttgatg tacttgccca
    25141 ccaggtcgct gttgacgcgg gcgacgaaat cgtccgggtt gaagtcgacg tcctcgacct
    25201 tgctgctgag cttcgtggcg atgtagtagc gcagccattc ggcattcatg ccgagctcga
    25261 ggtacttcaa cggcgagatg ccggtgccgc ggctcttgct catcttctcg ccgctgacgg
    25321 tgatgaatcc gtgcgcatac acccggtccg gcaccttgcg gcccgagaac tgcagcatcg
    25381 ccggccagaa cagcgtgtgg aagtaggtga tgtccttgcc gatgaagtgg atctgctcga
    25441 ccgccggatc ggcgatgaac tcgtcgaagc tgcgctgctc gccacgcacc ttcgccttgc
    25501 cctggtcgaa gtagttcttc agcgacgcga ggtagccgac cggcgcgtcg agccacacgt
    25561 agaagtactt gcccggcgcg tccgggatct cgatgccgaa gtagggcgag tcgcggctga
    25621 tgtcccagtc gctcaggccg ccggctccca gctcgtcctt cgtgaaccac tcgcggatct
    25681 tgttgcccac ttcgctctgc agccgctcgc cgccggtcca ctgctcgagg aactcgacgc
    25741 agcgaggatc gctgagcttg aagaagtagt gctcgctgct cctcatcacc ggagcggccc
    25801 cgctgagcgt cgagtacgga ttcttcagtt cggtgggtgc gtagaccgcg ctgcacacct
    25861 cgcagctgtc gccgtactga tcccgcgctc cgcacttcgg acattcgccc ttgatgtagc
    25921 ggtcgggcag gaacatgccc ttcaccgggt cgtagaactg ctcgatcgtc ctggtggtga
    25981 tcagcgagcc gtcggcgtgg tcgcgcagag ccctgtagat gccttgcgcg agttcggtgt
    26041 tctcgggcga gtcggtggag taccagtggt cgaacgcgat gtggaacccg tcgaggtact
    26101 gctggcgccc cgcggcgatc tcggcgacga actgctgcgg cgtctttccg gccttctcgg
    26161 ccgcgatcat gatcggtgca ccgtgcgcgt catcggctcc gacgaagtgg acttcatggc
    26221 cccgcatcct ctggaagcgc acccagatgt cggcctggat gtattccatc atgtgcccga
    26281 tgtggaacgg cgcgttggcg tagggcaggg cggtggtga
//
LOCUS       DAOORV010000040        26241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2223_length_26241_cov_7.911365, whole genome shotgun sequence.
ACCESSION   DAOORV010000040 DAOORV010000000
VERSION     DAOORV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2223_length_26241_cov_7.911365"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="carB"
                     /locus_tag="PKV56_07435"
     CDS             complement(<1..423)
                     /gene="carB"
                     /locus_tag="PKV56_07435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326830.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOX67685.1"
                     /translation="MPKRTDLKSILIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPITWQVVEKIIAKERPDAVLPTMGGQTALNCALDL
                     HKNGVLSKYNVEMIGANEKAIEKAEDRLKFKDAMTGIGL"
     gene            complement(416..1603)
                     /gene="carA"
                     /locus_tag="PKV56_07440"
     CDS             complement(416..1603)
                     /gene="carA"
                     /locus_tag="PKV56_07440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOX67686.1"
                     /translation="MLPVLPPAILVLADGSRFIGNSIGSPGQTCGEVVFNTALTGYQE
                     ILTDPSYCRQIVTLTYPHIGSYGVNTEDVESDRVHAAGLIIKDLPVLASNFRMTETLP
                     QYLQREGTVAIANIDTRRLTRLLRTSGAQNGCILTLNVGETLVEAHFEQALVAARAAP
                     SMAGLDLAKVVTCGARAVWTETEWALGLGYASLTEPRFHVVALDFGVKRNILRMLASR
                     GCKVTVVPAQTPASEVLALKPDGIFLSNGPGDPQPCDYAIEATRALIESGIPTFGICL
                     GHQILALASGAKTFKMKFGHHGANHPVKDLDSGRVSITSQNHGFAVDETTLPNNLRPT
                     HVSLFDGTLQGLARTDRPAFCFQGHPEASPGPHDIGYLFDRFTALMESQAKNAAVAGG
                     QHA"
     gene            complement(1767..2174)
                     /locus_tag="PKV56_07445"
     CDS             complement(1767..2174)
                     /locus_tag="PKV56_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOX67687.1"
                     /translation="MISRPALTLADVKRMASACEQEAVAHQWAVSIAIVDDGGHLLWF
                     QRLDGAAPVSAHIAPAKARTAALGRRESRIYEELINGGRVSFLSAPEIDGLLEGGVPV
                     IAEGHCIGAVGVSGVKSSEDAQVAKAGIAALGT"
     gene            2351..3040
                     /locus_tag="PKV56_07450"
     CDS             2351..3040
                     /locus_tag="PKV56_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOX67688.1"
                     /translation="MNQTLRAGYVGPITSVEQRNRVLRNTYWLLALSMVPTVLGAWIG
                     VSTEIAQLMTPGISLIVFMGGAFGFMYAIEKTKNSASGVPVLLAFTFFMGLMLSRMIG
                     AVLGLSNGASLIMMAFAGTGAIFLGMATLSTVIKRDLSSMGKFLFIGMIMLLVAGIAN
                     IFIQSSALMITLSVLAIGIFSAFILHDLKRVRDGEETNYITATLGVYLSIYNVFQSLL
                     AILGIAGGNND"
     gene            complement(3098..4465)
                     /gene="rlmD"
                     /locus_tag="PKV56_07455"
     CDS             complement(3098..4465)
                     /gene="rlmD"
                     /locus_tag="PKV56_07455"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOX67689.1"
                     /translation="MAQEDQDMTSNSEWLSVESLDLEGQGVAHNSEGKVVFIEGALPG
                     EQVQVSVFRRKNNWEQASISALRRESSQRVTPGCRYFGVCGGCKMQHFQAGAQLATKQ
                     RALEDGLWHLAKVKPDQVLRPIEGPAWGYRYRARLSVRYVAKKGKVLVGFHERKSSYV
                     ADMGSCDVLPAHLSALLTPLAELIGTLDQRERIPQIEVAVGDDTTALVLRHLEMLSSA
                     DQQRLRDFAACHRVQWWLQPHGPSTVHTLDASVASLSYSLPEFGITMRFKPTDFTQVN
                     HQINRVLVARAMRLLDPQPDERVIDWFCGLGNFTLPIATRAREVLGIEGSEPLVERSR
                     ENAAFNGLSATTRFAASNLFEIDAPALAALGHADKWLVDPPREGAFALAKALADVAEN
                     PAAAPGYRLPRRIVYVSCNPATLARDAGLLVHQAGYVLSAAGAVNMFPHTAHVESIAV
                     FDREG"
     gene            complement(4516..5457)
                     /locus_tag="PKV56_07460"
     CDS             complement(4516..5457)
                     /locus_tag="PKV56_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOX67690.1"
                     /translation="MNEARRHGAGLLAAVCLGLLLTACASRPNRAPVEDRQASTKAPA
                     PAAPASSEPQAEVPAAAAVTDNAGKPGYYTVKPSDTLLKIALDNGQNWKDLARWNGLD
                     NPNIIEVGQVLRVIPPGADSATPAARPVAAAKVESKPLDARAPAPAPTTVPAAASSGA
                     AVAAAPAAASAAAPAAVVVAPVVPAALPSGDEALPWMWPASGPVTTAFDESKSKGLAI
                     SGKAGDPVLAAADGRVVYAGSGLRGYGNLVIVKHNNTYLTAYAHNQTLLVKEDQAVRR
                     GQKIAEMGSTDAERVQLHFEIRKQGKPIDPAKLLPSR"
     gene            complement(5484..6302)
                     /locus_tag="PKV56_07465"
     CDS             complement(5484..6302)
                     /locus_tag="PKV56_07465"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOX67691.1"
                     /translation="MVRAPKTWVGPAVEANDAAKSGSSAAPGPSTQARGRSAALQAGN
                     SSARHRPAGLGLDSEDVRRRMVERLRVGGPYHAHVLDALASVPRHRFIDAALVTQAYE
                     DTSLPIGHGQTISKPSVVARMLDVLMDGVTARAAGHLGRVLEIGTGCGYQAAVLCCLS
                     RHVVSVERIKGLHDKARDLVAPLRMNQLRLVYGDGMLGHPPNAPYDSIIAAACGSSVP
                     ESWLTQLAVGGRLISPVDGATKGQMLVVVDRHEGGYQRTEFEAVQFVPLKSGSI"
     gene            complement(6350..7108)
                     /gene="surE"
                     /locus_tag="PKV56_07470"
     CDS             complement(6350..7108)
                     /gene="surE"
                     /locus_tag="PKV56_07470"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOX67692.1"
                     /translation="MRILIANDDGYLAPGLAALVAACEGLGHIDVIAPEQNASGTSNS
                     LTLNRPLTACTAANGFHYLNGTPSDCVHVALTGLFADRPDLVVSGINQGANMGDDTIY
                     SGTVAAAMEGYLFGIPSIAFSQVDKGWLHLDAAARVARGVIEQVLRDPPARRPFLLNV
                     NIPNCADADRLPRCITRLGRRHASEPVIRQQNPRGETMYWIGAAGDAREAGEGTDFHA
                     TTHGRVSITPLQVDLTDHVALPDWRAWLTSSESS"
     gene            7235..7978
                     /locus_tag="PKV56_07475"
     CDS             7235..7978
                     /locus_tag="PKV56_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67693.1"
                     /translation="MAVRVLIVEDNPVARSFLARVVRESFSDQLLISEASDLESARRL
                     IGDTQTTAASAGSAPDGLFKLMMVDLELPDGNGLELLAELSRDPATKIVTTLYSDDEH
                     LFPALQCGADGYLLKEDRFEVLVEELQKIVRGQPPLSPAIARRLLTHFRQGGSADNPA
                     ASSEAPALQSRGSSTLERSPHVDQERLTPRESEVLTYLSKGFTIKEIANLAGIKWFTV
                     NDHIRSIYKKLNVTSRAEAAVLASKQALA"
     gene            8126..9016
                     /locus_tag="PKV56_07480"
     CDS             8126..9016
                     /locus_tag="PKV56_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOX67694.1"
                     /translation="MNTKPSAIQAAAIDYGRPVAGWRLRLYTIIFEADTRAGRNFDLT
                     LIGCILASVVVVILDSMAAVHARHAATLGVLEWGFTLLFTAEYIARLVCVRHPERYAR
                     STFGIIDLLAVLPTYLAVLVPGLHALIDVRVLRLLRLFRIFKLGAYVAEFGALGQALA
                     ASRRKIFVFMSFVMLVVVVMGTLMYVVEGPDNGYTSIPVGIYWAITTMTTVGFGDITP
                     KTDLGRLLASVMMLLGWGTLAVPTGIVSAEFTSRRVAQSTTTRTCHDCLSEGHSPSAA
                     FCRDCGAPLPPWQRDGSPSD"
     gene            9488..9829
                     /locus_tag="PKV56_07485"
     CDS             9488..9829
                     /locus_tag="PKV56_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67695.1"
                     /translation="MSSPNVESTRSSIHTAFSLAHLLESIDRSSAPVDANQYRMVVGR
                     LKASLSNPLPEAALTALLNSYPSAAEVYETMHYGVAGLSRSPLERSVASESLARQVIS
                     RFAPGFKASGT"
     gene            9958..10791
                     /locus_tag="PKV56_07490"
     CDS             9958..10791
                     /locus_tag="PKV56_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67696.1"
                     /translation="MAPINPRITPLLRSVTALEVAVLTIAGLGLLLDVAAVTGVWPWP
                     LKPFNQRFLGALYSAALVAAVWQVAIGRWAPARSITWMILVFTAVVTALSFLHLSRFD
                     FASPPAWIWFVLYVGVCVNAGLHLYWPRGQTPVGQRPTRRWQLVLAMQAAVCFALGLA
                     HLVKPEMSARYWPWPLDTFHTQLYSVVFLTPAVAAWCLRRAATPADWRAQGSTQIAWG
                     LLPLVGLLLADAGVRRVDWSAPSTLAWTGVFALLAVIGLAMWHNADEGPAAPRTSGPR
                     T"
     gene            complement(10849..11613)
                     /locus_tag="PKV56_07495"
     CDS             complement(10849..11613)
                     /locus_tag="PKV56_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOX67697.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAIAARQGQRTLLIDLDRQANSS
                     RYLMGDAVDESMPGAAEFFDATLKFSVRSPDTVSFISETPWENLHLMPASGLLDELHG
                     KLESRHKIYKLRDALVELEGEYDSIWIDTPPALNFYTRSALIAAQGCLIPFDCDDFSR
                     RALYGLLESIKEIQADHNPDLVIEGIVVNQFQPRASLPQRTVQELIDEGLPVLEPYLS
                     ASVKIKESHEMSCPMIHLDANHKLTQQFVALYESLG"
     gene            11803..13614
                     /locus_tag="PKV56_07500"
     CDS             11803..13614
                     /locus_tag="PKV56_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HOX67698.1"
                     /translation="MTALYSLPLHLLIAGGALAALLISFLAFFLLPGLLHWWRLRRIE
                     REIGRLDPRSPPAELRRIFARDRRLAQLWKEFEDSLHVQREERGGQPAPVAVRSTLPA
                     ETFFNGPCVVDARLRTEFFKHLPGIYTGIGIIGTFSGLIDGLRQFQVSENAATVRSSL
                     ESLMHAVGQAFLISATAIAAAIVVTFLEKLLLSALYGRTEDIAHAIDARFDAGAGEDY
                     LSRLVQATEGAAGRSLVLKDALVAELAGVLREQTDRQISGLASAITNSIERSLAAPLQ
                     KIASAVQASTSDQSALTTQLLQDVMGRFGDRLDNVLGGQVSGINALNQQTGQTMQEAV
                     QAVRALVEQISQSSRQSNDSMTANLVDATKAMLAALGESQRVSVEGQQRRELALTERS
                     SAAMNDMTDLLARTLDEVRFASSQMAGSIDAMKFMTTAALDRMSSGAAQLSTASQTFS
                     TAGDRVSGVMEQAALVSSQMTEASRLMLASAADMRSGLDDYRAHRDAVGHLVTELRTT
                     VEHARTEATLTADVLDRIKVSTERLGQAQEHADLYLASVSKVLGDAHQAFAIAVKKTL
                     DLANTEFHSKLSTAVGLLSSTVVELEATLGNVTPQRH"
     gene            13617..14327
                     /locus_tag="PKV56_07505"
     CDS             13617..14327
                     /locus_tag="PKV56_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX67699.1"
                     /translation="MLFAQRQARRAPRDEAEKPFWISFADLMTALMVLFLVVMGVALL
                     AITKNVTDREQQQEQRRKDIEAVFDQLGKTAARHEGVRIDRDRHVIDFGDRARFAFNK
                     STLAAEQEIVLRRFVPELIGLADSPLGRKVMKRIVVEGYADRTGSYLSNLNLSLQRSQ
                     RVLCAMFATRGDSLLDDRQKASVRDLFLVGGYSFNASKASDEESRRVEMRLEFLGMGE
                     QRAAPEAAPGSFGECALP"
     gene            14312..16060
                     /locus_tag="PKV56_07510"
     CDS             14312..16060
                     /locus_tag="PKV56_07510"
                     /inference="COORDINATES: protein motif:HMM:NF026949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EH signature domain-containing protein"
                     /protein_id="HOX67700.1"
                     /translation="MRAALNPVDHLASLLAPAVRLAGRAMPQSGEIDTVIAALQRQIK
                     SGSRRPLNADLQHDAAQRFWRSPRLQTLKDARLVAFGLGLPVGPGGARLIDDRARFDA
                     VLRGVDQWLNDARWFRRCYQGLVWSYFNCDIDTSLHHSDARHNWLRLRDYLSQRAPRT
                     VSPESNPDWVKTAICQRHLFGDAACERHAGDLLRGHHQEVQSICEQLGINGSSWFLRG
                     LVLAQVSAATKLDHDEFRAALPRLIELLSDTRALRDEGLALLLNRYQQLPQAPLHEGL
                     RDAAASWWGSPWLATNEFRWSGMSDGVRRMVSEWIQCDLIDRFFAAAGDGPSGRRAAF
                     WKRYVKSNRLIGFALGGSTEQAEQSRARLGVQRVTGPCASLVDGSLTHSALVLTMGRA
                     VIVEFGDLSVPLHGYDLRHSQPFDLSCPMSLEEDAGNSLRQRHRSLLLPHQDGLEGWR
                     RWEQMFEAAISDQFGVRPGTAPSTDETSCVDLSDPADRIDRGDPEPLGQIDEPRWQTS
                     SSGEDAHWLTAEAASVPYSRADLEVLARVHALRIEDDSSRSGRLWVRTDQGDHRIARV
                     LTGWGFVHRPGEGWLR"
     gene            complement(16093..16467)
                     /locus_tag="PKV56_07515"
     CDS             complement(16093..16467)
                     /locus_tag="PKV56_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67701.1"
                     /translation="MFTLDTCCTLVPYFEVPDGQLAAFKALGPQFVAKTRTESGCRHY
                     AFSFSGGTAHCREGYDNADAVLAHLQNVGELLGEALKLAKLVRLEVHAPAAEIEKLRA
                     PMAGLNPQFFVLEEGIRRTSEA"
     gene            complement(16597..17019)
                     /locus_tag="PKV56_07520"
     CDS             complement(16597..17019)
                     /locus_tag="PKV56_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67702.1"
                     /translation="MRLALFVLSIASSLPALAQTQPPEPSRIEFPSVAVALKELQARD
                     GNGTVVVHADGWTIINEPLASAQWSFAPDGHHAYPSVVRRIIRRSSAGSVSVETASLC
                     EAPQAECSKLLSEFAAMDERITQSIKARGRQGSTQPAQ"
     gene            complement(17057..17536)
                     /locus_tag="PKV56_07525"
     CDS             complement(17057..17536)
                     /locus_tag="PKV56_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX67703.1"
                     /translation="MSTLEWAPELSLDMPVMDRTHREFVELLAAVEQAPDDQLFGAWD
                     ELIEHTDIHFAREDRWMQDTGFSMPNCHSAQHAMVMKVMNEGAQLKRMDIIRTMAREL
                     AMWFPEHARNMDAALADHLKSVGYDVSSGSVAQPQALPQTAISGCGSAACTPAAETQ"
     gene            complement(17650..18867)
                     /gene="ubiM"
                     /locus_tag="PKV56_07530"
     CDS             complement(17650..18867)
                     /gene="ubiM"
                     /locus_tag="PKV56_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HOX67704.1"
                     /translation="MTPSHSLPARDADVLIVGAGPAGLTLACALGDIGLSVLLLEQQP
                     EGALVDPPEDGREIALTHRARRVMEQLGLWRHLPADEIAPLREARVVDGDAPTVLRFE
                     SSGVDGSALGWLVPNHLIRRAAWAAAAERQGVRCVCDARVTALHPTADQTGVTLSDGR
                     RLNALLVVAADSRFSQMRRLAGIGASMHDFGRSVVVGRVAHEQDNGGVAWECFRYGNT
                     LALLPLNKGQSSAVVTVASDQAPHWMALADAEFAARVDAQSDRRLGVTATAGPRHLYP
                     LVGVYAHRFVAARLALMGDAAVGMHPVTAHGWNFGLYGVEVLARELAASLAAGRDIGA
                     LPALQAYEREHRRTTLPVYLGTNAIVSLFCDERALPKLARRAVLTLAEKVPPFSGLIK
                     AAITRQLVGAVRS"
     gene            complement(18973..19455)
                     /locus_tag="PKV56_07535"
     CDS             complement(18973..19455)
                     /locus_tag="PKV56_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67705.1"
                     /translation="MSAISRKPEFSLEWVEEDETTQTARLLPTSERAKAWLEQHGMPA
                     VLARADACAREGQPLEEILQDLLEEDAGFAGTMPAPVADLTPGTCGEFRVEFVSDPAD
                     SGRKIARVTGLTERARGWMKFQGQSLGLRLSREQRPGERLDALHQRLLEQDLGNALHV
                     "
     gene            complement(19499..19975)
                     /locus_tag="PKV56_07540"
     CDS             complement(19499..19975)
                     /locus_tag="PKV56_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csp1 family four helix bundle copper storage
                     protein"
                     /protein_id="HOX67706.1"
                     /translation="MQRRSFIGSITSLAAAGLGAVTAGRAQAQEPHQHDHKAMMAARG
                     AAAPRRYDDLVEPFQACSKAVAICISHCQALLAAGDRSLGRCLRTALDCDVVCNATLR
                     AALINSSYTPSLAKTSIKAMEACVKACMPHVEHHAECKDCHDACLAAIDAVRKLSA"
     gene            complement(20084..21418)
                     /locus_tag="PKV56_07545"
     CDS             complement(20084..21418)
                     /locus_tag="PKV56_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOX67707.1"
                     /translation="MKTRIAASKARNISPQEAAGFVASGMWIDYGASLCQPDVFDAAL
                     ARRTDELHGVKIRACLTLRPRAVLEADPQGEHFFWINLHFSGYDRRKHDAGIANYLPV
                     NLGEIPDYYRRFIDPVDIVIIKTCPMDADGYFNFSAATVWHRAVIERARMVIVEVSSG
                     LPYVLGEQTGVHVSEVDYIIEGDSAPATELPNPPPTEVDEAVARLIAGEIEDGDCLQI
                     GIGGMPNAVCSLLSGSGVHDLGIHTEMLTDGIMDLYQAGRITGARKQLNPGKIVCTFA
                     LGSRRLYDGIDRNPDIHFYPVDYTNLPHNIMRNDRVIAINNTTQIDLQGQAASESDGH
                     RHISGTGGQLQFVRGAYASNGGKSFVCLASTYEKRGARRSRIVLSLTSGNIVTTPRSD
                     MMYVATEYGMTNLKGKSVAERARALISIAHPDFREELERSAHEHRLIPRGFF"
     gene            21676..22899
                     /locus_tag="PKV56_07550"
     CDS             21676..22899
                     /locus_tag="PKV56_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX67708.1"
                     /translation="MTAAVPRRVGLYVAVVQFFFALTWSVYVIYLPQLAAQAGIPKTA
                     VIWLLMLDQLVFLVADYACGVASDRMARLNSRIGPAVLAVTVLSCAAFLLLPFIAPRG
                     SALLFIAVTVLWTVGSSALRAPPLNIIGRHAARPSQPALVALSMLGLGLAAAAAPYLA
                     TALREVDPRWPFALASISLAATTLGIIAAERALQGRPAASVTSAPDALVKSDPRRLPW
                     APCLVAAVLAAIAFQVHVFLNSTPLYLRHANSGDIPQLAPVFWIGFNLGLWPASLAIK
                     RHGGIAVTAAAGLTAAVASAWAAGAGSIETLVAAQALAGAAWAAVLTSAFSAALALGH
                     TGAEGRFSGALSSTLALAALLRMGLLASGLTSLPHDSTMRLALDWLPAALWLLAGALL
                     LAIWQREAARTRFRP"
     gene            23134..>23876
                     /locus_tag="PKV56_07555"
     CDS             23134..>23876
                     /locus_tag="PKV56_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67709.1"
                     /translation="MSADRRRLTFALLFSLLIHALLLSLTFGGQGLGLPGFGFPWEER
                     RVEVPDLRIVILPTPVTAAEPAISSVDESRLQASIEQVVAGEPTQTASPPPAPASEKT
                     ADAVPPKTRRAAAAKPKAETSPEPDPTTGAAADEAPVPAEEPGEVAPAAIPDAPVIAV
                     DRPDEATFVVSAAASEPTPVIAAAPAASSPETALPKPRAAGDAVRERVDPNALERAVE
                     LARVERSEQEAARQEAQRIEIARLEAERQ"
     assembly_gap    23877..23976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23977..25487
                     /locus_tag="PKV56_07560"
     CDS             <23977..25487
                     /locus_tag="PKV56_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67710.1"
                     /translation="RQEAQRIEIARLEAERQEAVRQAAARQEAARLEAQRIETARLDA
                     ERQEAARQAAARQDASRQEAQRIEAARLDAERQETARQAAARQEASRQEAQRIEAARL
                     EAERQEVARQAAARQEAARQEAQRIETARLDAERQEAARQAAARQDASRQEAQRIEAA
                     RLDAERQETARQAAARQEASRQEAQRIEAARLEAERQEVARQAAARQEAARQEAQRIE
                     AARLEAERQEAARQAAARQEAARQEAQRIEAARLDAERQETARQAAARQEAARQEAQR
                     VEAARLEAERQEAARQAAARQEAARQEAQRIENARLDAERQEAARLEAARQAVARQEA
                     AKREAALEDDARREASRRAMGRQLDEEAARREAASASTAAGMPSRLPPSSSGARRGRL
                     FGRIDPNAELVLYAEAWARKIQLNMTFDMVREVVKRPHADPLVTVAIRSDGSVESVTF
                     VLSSGVPEIDEAIRGILQGQAPYQPFQPALAREFDVIEIRRSWHFDMAIRLY"
     gene            complement(25528..26202)
                     /locus_tag="PKV56_07565"
     CDS             complement(25528..26202)
                     /locus_tag="PKV56_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOX67711.1"
                     /translation="MPDYLGACDQPVEGLRIAWSRTLGYARPDPEIADIAQRAAQRFS
                     EMGCEVVEVDRVLAQDPIDLWASEFYAGVGVRLKKSLIEQLELLDPAVADIVGRALEQ
                     TTETYCTRVFQRYELRETMRRFFERFDLLLTPTLPCAAFDVGLNTPPQWPDRNVISWV
                     YYTYPFNLTGQPAASVPAGWTRAGLPVGLQMVSRPHRETDIFRAAAALERCQPWADRR
                     PNVTLI"
BASE COUNT         4343 a         8723 c         8685 g         4390 t
ORIGIN      
        1 caggccgatg ccggtcatcg cgtccttgaa cttcagccgg tcctcggcct tctcgatggc
       61 cttctcgttg gcgccgatca tctcgacgtt gtatttcgac agcacgccgt tcttgtgcag
      121 atcgagtgcg cagttcagcg ccgtctggcc ccccatggtg ggcagcacgg catcgggccg
      181 ctccttcgcg atgatcttct cgaccacctg ccaggtgatg ggctcgatgt aggtcgcatc
      241 ggccatctcc gggtcggtca tgatcgtcgc cggattgctg ttgacgagga tgacgcggta
      301 gccctcctcg cgcagcgcct tgcaggcctg ggctccggag taatcaaatt cgcaggcctg
      361 gccgatgatg atcggcccgg cgccgatgat caggatcgat ttgaggtcgg tgcgcttagg
      421 catgttgacc tccagccact gcagcgttct ttgcctgaga ttccatcaac gcagtgaagc
      481 gatcgaacag gtagccgatg tcatgcggcc cgggcgatgc ttcagggtgg ccctggaagc
      541 agaacgccgg acggtcggta cgcgccaggc cctgcaaggt gccatcgaac aggctgacgt
      601 gggtcggtct caggttattc ggcaaagtgg tctcgtcgac ggcaaagccg tggttctggc
      661 tggtgatgct cacgcgcccg ctgtcgaggt ccttgacagg gtggttggca ccatggtggc
      721 cgaatttcat cttgaaggtc ttggctccgg aggccagcgc caggatctgg tgccccaggc
      781 agattccgaa ggtcgggatg ccactttcga tcaatgcgcg agtagcttcg atggcgtagt
      841 cgcaaggctg cgggtcgccc gggccgttgc tgaggaagat gccatccggc ttcagcgcaa
      901 gcacctcgct cgccggtgtc tgagccggaa ccacggtcac cttgcagccg cggctggcaa
      961 gcatgcgcag gatgttgcgc ttgacgccga agtcgagcgc gacgacgtgg aagcgcggct
     1021 cggtgaggct cgcgtagccc aggccgagcg cccactcggt ttcggtccag acggcgcgag
     1081 caccgcaagt cacgaccttc gccagatcga gtccggccat gctgggagcc gcacgcgcgg
     1141 caaccagagc ctgttcgaag tgcgcctcca ccagcgtctc gccgacgttc agggtcagga
     1201 tgcagccgtt ctgtgcgcca ctcgttcgca gcagtcgggt gaggcggcgt gtgtcgatgt
     1261 tcgcgatcgc caccgtgccc tcgcgctgca gatactgtgg cagcgtctcg gtcatgcgga
     1321 agttcgacgc caggacgggc aggtccttga tgatcagacc ggcagcgtgg acccgatccg
     1381 attcgacatc ctcggtattg acgccatagc tgccgatgtg cgggtaggtc agcgtgacga
     1441 tctggcggca gtagctggga tcggtgagga tttcctggta gccggtgagc gcggtgttga
     1501 aaaccacctc gccacaggtc tgtcctgggg agccgatcga attgccgatg aaccgggagc
     1561 cgtctgcgag aacaaggatt gcggggggca ggacgggcaa caaggaaatc tccgttggtg
     1621 cacccagctc tgccccgcag cacgggctgc gagtcggaat ccggtgggaa acttggaagg
     1681 gtttcgctgg gggcgggtgt gttcgggtaa acctccggat tgtaatgggt gaccagcagt
     1741 cgaaccccga acgccccttc cgaaagtcag gtgccgaggg ccgcgatgcc cgccttggca
     1801 acctgcgcgt cttcgctgct cttcactccg ctgaccccca ccgcgccgat gcaatgcccc
     1861 tccgcgatca ccgggacacc tccttcgagc agtccatcga tctcgggtgc gctgaggaaa
     1921 gacacccgcc ccccgttgat caactcctca tagatgcggc tctcgcggcg tcccagcgcg
     1981 gcggtccttg ccttggcggg cgcgatgtgc gccgagacag gcgctgcacc atcgagccgc
     2041 tgaaaccaca acagatgtcc gccatcgtcg acgatggcaa tcgacacggc ccactggtga
     2101 gcgactgcct cctgttcgca cgccgaggcc atgcgtttca cgtcggccag ggtcagagca
     2161 ggtctggaga tcatggcgca ccaccttgaa aaagccaaaa cccgactgta tccgccgtgg
     2221 tttgcgcgat tcggaaccac cttgccactg gcttggaggc cgctctcccc atcagcgcga
     2281 cgcgcttggc ggggaactag aatccggccg gcgagtctca ccgcctgtct catttccacg
     2341 ggagtcctgt atgaaccaaa cactgcgcgc cggctatgtc ggcccgatca catcggtgga
     2401 gcagcgcaac cgcgtccttc gcaacaccta ctggcttctg gccctgtcta tggtgcccac
     2461 ggtgctgggc gcatggatcg gagtctcgac cgaaatcgcc caactcatga ccccgggcat
     2521 cagcctgatc gtgttcatgg gcggcgcatt cgggttcatg tatgccatcg agaagaccaa
     2581 gaactcggcg tccggtgttc ccgtgttgct ggctttcacc ttcttcatgg gcctgatgct
     2641 gtcgcgcatg atcggcgccg ttctcggcct gtccaacggt gcaagcctga tcatgatggc
     2701 gtttgcaggc acaggagcaa tcttcctggg catggcaacg ctgtcgaccg tgatcaagcg
     2761 cgacctgtcg tcgatgggca aattcctttt catcgggatg atcatgctgc tggtggcagg
     2821 catcgcaaac atcttcatcc agtcgagcgc cttgatgatc acgctgtcgg tgctggcgat
     2881 cgggatcttc tcggccttca tcctgcacga cctgaagcgt gtcagggacg gagaggaaac
     2941 caactacatc accgcaacgc tgggcgtgta cctgagcatc tacaacgtgt tccagtctct
     3001 gcttgcgatc ctgggcatcg ctggcggcaa caacgactga ttactgtcgg caggacactg
     3061 aaagacgggg ccttcgggtc ccgtttttct tggttggtca tccttcgcga tcgaacaccg
     3121 cgatgctctc gacatgggct gtgtgcggga acatgttgac ggctcccgca gctgacaaca
     3181 catatccggc ctggtgcacc agcaagccgg catcgcgagc cagcgtggcc gggttgcaac
     3241 tgacgtagac gatgcgcctg ggcaatctgt agccgggagc cgcagcggga ttctccgcaa
     3301 cgtcggcgag ggccttggcc agggcgaatg ccccttcgcg cggaggatcg accagccact
     3361 tgtcggcatg accaagcgct gccaacgccg gcgcatcgat ctcgaacagg ttgctggccg
     3421 cgaatcgggt cgtggcggac aagccgttga acgcggcgtt ctctcgcgag cgctcgacca
     3481 acggctcgct gccttcgatt ccaagcacct cgcgcgctcg agtcgcgatc ggcaacgtga
     3541 agttgcccag gccgcagaac cagtcgatca cgcgttcgtc gggctgtgga tcgagcagcc
     3601 gcatggcgcg tgccacgagc acgcggttga tctggtgatt gacctgagtg aagtcggtcg
     3661 gcttgaatcg catcgtgatg ccgaactcgg gcaggctgta cgagaggctc gccaccgaag
     3721 cgtccagcgt gtggaccgtc gagggaccgt gcggctgcaa ccaccactgc acgcgatggc
     3781 aagccgcgaa atcccgcagt cgctgctggt cggccgaact cagcatctcg aggtgccgca
     3841 gaacgagtgc agtggtgtca tcgccaaccg ccacctcgat ctgggggatc cgttcgcgct
     3901 ggtccagcgt gccgatcaat tcagccagcg gagtgagcag cgcactcaga tgcgcaggca
     3961 gcacatcgca actgcccatg tcggccacgt agcttgactt gcgttcgtgg aatccgacca
     4021 gcaccttgcc ctttttcgca acgtagcgca ccgaaagccg ggcgcggtag cggtagcccc
     4081 aggcgggccc ttcgatcggt cgcagcacct gatcgggctt gaccttcgca aggtgccaca
     4141 ggccgtcctc gagtgctcgc tgcttggtgg cgagctgcgc gccggcctgg aaatgctgca
     4201 tcttgcagcc accgcagacc ccgaaatagc ggcagcccgg ggtgactcgc tgcgaactct
     4261 cccgacgcag cgcggagatg gaagcctgct cccagttgtt cttgcggcgg aagacgctga
     4321 cctgcacctg ctcacctgga agcgcgccct cgatgaagac caccttgcct tcggagttgt
     4381 gggccacgcc ctgaccttcg aggtcgagcg attcgacact cagccattcg gagttcgatg
     4441 tcatgtcttg atcttcttgg gccagcagcc tcgacccgac tgcgggcccc ggacggacgg
     4501 ggcggcaggc ggcgatcagc gtgaaggcag cagcttcgcg ggatcgatgg gtttgccctg
     4561 cttgcggatc tcgaaatgca actggacccg ctcggcatcg gtcgaaccca tctcggcgat
     4621 cttctgcccg cgtcgcaccg cctggtcttc cttcaccagc agcgtctggt tgtgcgcgta
     4681 ggcggtgagg taggtgttgt tgtgcttgac gatcaccaga ttgccataac cccggagccc
     4741 cgatcccgcg tagaccacgc ggccgtcggc cgcggccagt acggggtcgc cagccttgcc
     4801 tgaaatggca aggcccttgc tcttggattc gtcgaacgcc gtggtgacgg ggccgctggc
     4861 aggccacatc caggggagcg cctcatcgcc cgaaggcagc gcagcgggaa cgaccggggc
     4921 gacgacgacc gccgcgggag cagcagcgct ggccgctgca ggcgctgctg ccactgcggc
     4981 ccccgaactg gccgcagcgg gcaccgtcgt cggtgcagga gccggggcgc gagcatcgag
     5041 gggtttgctt tcgacctttg ccgccgccac cggccgagct gctggcgtcg cggaatcggc
     5101 gccaggcggg atgacccgca acacctgtcc gacctcgatg atgttggggt tgtcgaggcc
     5161 gttccagcgc gccagatcct tccagttctg gccgttgtcc agggcgatct tcagcagggt
     5221 atccgacggc ttgacggtgt agtagccggg cttgccagcg ttgtcggtca cagcagcggc
     5281 ggcaggcacc tcggcctgag gctcactgct tgccggtgcg gccggcgcgg gtgccttcgt
     5341 tgaagcctgt cgatcctcga caggcgcgcg gttaggtctc gacgcgcacg ccgtcaacaa
     5401 caatcccagg cagacagcgg ccaacagacc cgccccgtgt cgtcgagcct cgttcatcgt
     5461 tctcatccgg gcggatacct cgttcaaatg gaccctgatt ttagagggac gaactgcacg
     5521 gcttcgaact cggttcgctg gtatccccct tcgtgacgat ccacgacgac cagcatctgc
     5581 cccttcgtcg caccgtccac cggagagatc aggcgtccgc caacggccag ttgagtcagc
     5641 cagctttcgg gcaccgatga accgcatgcg gcggcgatga tgctgtcgta gggtgcgttg
     5701 ggcgggtgcc ccagcatgcc atcgccgtag accagccgga gctggttcat ccgcagcggt
     5761 gcgaccagat cgcgcgcctt gtcgtgcagc cccttgatgc gctcgacaga caccacgtgc
     5821 ctgctcaggc agcagagcac cgccgcctgg tagccgcaac cggtacctat ctcgaggacc
     5881 cgaccgaggt gaccggcagc tcgagcggtc acgccgtcca tcaacacatc gagcatgcgt
     5941 gcgaccaccg acggcttgga gatggtctgc ccatggccga tcggcaggct ggtgtcctcg
     6001 taggcctgcg tcaccagcgc ggcatcgatg aatcgatgcc gcggaacgct cgccagcgca
     6061 tccaggacat gcgcgtggta cgggccgccg acacgcaacc gctcgaccat ccggcgccgc
     6121 acgtcttccg aatcgagtcc gagcccggcc gggcgatgcc gtgccgagga gttgccggct
     6181 tgcagcgcag cagaccggcc acgcgcctgg gtcgagggcc cgggggcggc ggagctgccg
     6241 gacttcgctg catcgttcgc ctcgactgcg ggaccgaccc aggtcttggg cgcgcgcacc
     6301 atgtgctcca gccgcagcgg gaacttgctg ctgcgacgcg atgactcggt cacgatgact
     6361 cggacgaggt caaccacgcc cgccaatcag gcaatgccac gtggtcggtc aggtccacct
     6421 ggagtggcgt gatcgacacg cgaccgtgcg tggtcgcatg aaaatcggtg ccctctcctg
     6481 cctcacgtgc atcacctgca gcaccgatcc agtacatcgt ctcgcctcgc gggttctgct
     6541 ggcggatcac cggctcgctc gcatgacgac gtccgagacg ggtgatgcaa cgcggaagcc
     6601 gatccgcgtc ggcgcaattc gggatgttca cgttcagcag gaaggggcga cgtgcgggtg
     6661 gatcgcgcag cacctgctcg atcacccctc gcgccacacg cgctgccgca tccagatgca
     6721 gccacccctt gtcgacctgc gaaaacgcga tcgacggaat cccgaacagg tacccctcca
     6781 tcgcggctgc aacggttccc gagtagatcg tgtcgtcgcc catgttggcg ccttgattga
     6841 tcccggaaac caccagatcg ggtcgatccg cgaacaggcc cgtcaaggcg acgtgaacgc
     6901 aatcggaagg cgtgccgttc aggtaatgaa acccgttggc cgcggtgcag gccgtcaggg
     6961 gacgattcag tgtcagcgag ttggacgtgc cactcgcgtt ctgctccggc gcgatgacat
     7021 cgatgtgccc cagcccttcg caggctgcga ccagtgccgc gagccccggg gccagataac
     7081 catcgtcgtt tgcgatcagg atacgcattg caacgattgt aggagtcgat cacacgcccc
     7141 ttcgttaagc tcgcggcaac gctcagcgat gcgcaggctt ctcggtgcgg gcctgctgct
     7201 agcatgcgcc gcactgcgaa tcggactcac acccatggcc gtcagagtat tgatcgtcga
     7261 agacaacccg gtggcccgca gcttccttgc gcgggtcgtt cgggaaagct tcagcgatca
     7321 actgctgatc tccgaggcca gcgatctcga gtcggcgcgc cggctgatcg gtgacaccca
     7381 gaccaccgca gcatcggcag gcagcgcccc cgatgggctg ttcaagctga tgatggtcga
     7441 cctggaactt cccgacggaa acggcctgga actgctggcc gagttgtccc gagatcccgc
     7501 caccaagatc gtcaccacgc tgtattcaga cgacgagcac ctgtttccgg ccctgcagtg
     7561 cggtgccgac ggctacctgt tgaaggaaga ccgcttcgag gtgctggtcg aagagctgca
     7621 gaagatcgtg cgcggccagc caccgttgtc tccggcgatc gcccgccgcc tgctgacgca
     7681 cttcaggcaa ggtggcagtg ccgacaaccc ggccgcatcg agcgaggcac cggccctgca
     7741 gagccgtgga tcctcgacgc tcgagcgttc gccccatgtc gaccaggagc gcctgacgcc
     7801 gcgcgagagc gaggttctga cctacctcag caagggcttc accatcaagg agatcgcgaa
     7861 cctggcgggc atcaagtggt tcacggtgaa cgaccacatc aggtcgatct acaagaagct
     7921 gaacgtcacg agccgcgccg aagcggccgt tctggccagc aagcaggcac tggcgtagtt
     7981 cgcccttccg gcggcctgcg ctgtcgccga tccaggccgg atcgggcggg gcccgcagca
     8041 acgcggggag aaccgatgca ggcacgccgg cgctcggcac gcgaaccggc ccggcgaggt
     8101 cacccttgcc ctaggatgct tgcccatgaa cacgaaaccg agcgcgatcc aggccgctgc
     8161 aatcgattac gggcgccctg tcgcgggctg gcggctgcgt ctgtacacga tcatcttcga
     8221 ggccgacacg cgcgccgggc ggaacttcga cctgacactg atcggctgca tcctggccag
     8281 cgtcgtggtg gtgatcctgg acagcatggc tgcggtgcat gcacgccacg cggcaacgtt
     8341 gggagtgctc gaatgggggt tcaccttgct gttcaccgcc gagtacatcg cccgcctggt
     8401 ctgcgtgcgc caccccgaac gctatgcgcg cagcaccttc ggcatcatcg acctgctggc
     8461 cgtgcttccg acgtacctcg ccgtgctggt gccggggctc catgcgctga tcgacgtgcg
     8521 cgtgttgcgg ttgctgaggc ttttccgcat cttcaagctg ggcgcctatg tcgccgagtt
     8581 cggagcgctg ggtcaagcgc tggcggcatc acggcgcaag atcttcgtct tcatgtcctt
     8641 cgtgatgctc gtggtggtgg tcatgggcac gctgatgtac gtggtggaag gcccggacaa
     8701 cggctacacc agcatcccgg tgggaatcta ctgggccatc accacgatga ccacggtcgg
     8761 cttcggcgac atcacgccga aaaccgacct gggcaggctg ctcgcttcgg tgatgatgct
     8821 gctcggctgg ggaacgctgg ccgttccaac cggcatcgtc agcgccgagt tcacctcgag
     8881 gcgagtggcg cagtcgacga ccacgcgcac gtgccacgat tgcctgagcg aagggcactc
     8941 tccgagcgcg gccttctgtc gtgactgcgg ggccccgctt ccgccctggc agcgcgacgg
     9001 atccccgtcg gactgagacc cccggacgcg cagcccagga tgcccgcccc atggtcggcg
     9061 cccgccggca aacgggccga atgaccggcc cgtccgtgac ccgcaactag cctttcgagg
     9121 tgctctgttc cggggtgccc tgcttctgct gcttggcctg tctcttcagt tccttggccc
     9181 gcttctgctt ttctctgcga ataccgcggt ctttgtcagc gctcatgtcg atctctggtt
     9241 ggttgatgag tggcacaggt gccgatccgc ctgggtcgac gcacgaccac aagcccatcg
     9301 ggacgatgtg cctccgattg tcgtcccagc agacacaccg aaggcgcccg aggcgatcca
     9361 ccgtgccctg acggtcatgc accgcacctg cagcgcacac gtgtcccgcg cccggcaccc
     9421 ttgactttat gccgacgaac cgcatgtaca gaaggcagcc agcccaacag gccccaggga
     9481 gtcatccatg tcttcgccca atgtcgagtc cacccgcagt tcgatccaca ccgcgttttc
     9541 actcgcgcac ctgctcgaga gcatcgaccg cagctccgcc ccggttgatg cgaaccagta
     9601 ccgcatggtg gtcgggaggc tgaaggcatc cttgtcgaat ccgctgcccg aagccgcctt
     9661 gactgccttg ctgaactcct acccttccgc cgccgaggtg tatgagacca tgcactatgg
     9721 cgtggccgga ctgtcgcgtt cgcccctgga gcgctcggtg gcttcggaat cgctcgccag
     9781 gcaggtcatc tcccggtttg cgccgggctt caaagccagc gggacctgaa agtcgccctc
     9841 ctcccgcatc cgcagggatg ccgggttccg aaagactgag acggggacct gacccggtct
     9901 gccgggcacg gcggcccgga aatttgaaga aggccgcagg aagtgcgaac ggacgacgtg
     9961 gcccccatca acccacggat cacaccgctg ctgcgcagcg tcaccgccct tgaggtggcc
    10021 gtgctcacga tcgccggcct tggattgctg ctcgacgtgg ccgcggtcac cggcgtctgg
    10081 ccctggcccc tgaaaccctt caatcagcga ttcctgggcg ccctgtacag cgcagccctg
    10141 gttgcagcgg tctggcaggt cgcgatcgga cgctgggccc cggcgcgctc gatcacgtgg
    10201 atgatccttg tcttcacggc ggtggtcacg gcactgtcgt tcctgcacct gtcgcgtttc
    10261 gactttgcaa gccccccggc atggatctgg ttcgtgctct acgtgggtgt gtgcgtgaac
    10321 gctggattgc acctgtactg gccgcgcggc cagacgcccg tcgggcaacg ccccacccgg
    10381 cgctggcaac ttgtcctggc gatgcaggcc gcagtctgct tcgcgctggg cctggcccac
    10441 ctggtcaagc ccgagatgtc ggcccggtat tggccgtggc cgctggacac cttccacaca
    10501 cagctctaca gcgtggtctt cctgaccccg gcagtggctg cctggtgcct gcggcgcgcc
    10561 gccacgccag cagactggcg ggcccagggc agcacccaga tcgcatgggg tctgcttcca
    10621 ctggtggggc tgctgctcgc cgatgcgggc gtgaggcgcg tcgattggtc tgcaccctcc
    10681 acgctggcct ggaccggtgt gttcgcgctg ctggccgtca tcggactggc gatgtggcac
    10741 aacgcagatg aaggcccggc tgcaccgagg acaagcggtc cgcgcacatg acccgtcggt
    10801 gcgtcgaacg caccctgctg cgcccgaccg aatcgatcgc cagcgaaatc agcccagcga
    10861 ttcgtaaagc gccacgaact gctgcgtcag cttgtgattc gcatcgagat gaatcatcgg
    10921 gcagctcatc tcgtgcgact ccttgatctt cacgctggcg ctcaggtagg gctcgagcac
    10981 gggcaggcct tcgtcgatca gctcctggac cgtccgctgc ggcaggctgg ctcgcggctg
    11041 gaactggttg accacgatgc cctcgatcac caggtcaggg ttgtggtccg cctgaatctc
    11101 cttgatgctc tcaagtaatc cgtaaagcgc ccggcgcgag aaatcgtcac agtcgaacgg
    11161 gatcaggcag ccctgggcag cgatcagcgc cgagcgggtg tagaaattga gcgccggcgg
    11221 cgtgtcgatc cagatcgagt cgtactcgcc ttccagttcg accagcgcat cgcgcagctt
    11281 gtagatcttg tggcggcttt ccagcttgcc atgcaactcg tccagcaacc cgctcgcggg
    11341 catcaggtgg aggttctccc agggcgtctc gctgatgaag ctgacggtat ccggcgagcg
    11401 cacgctgaac ttcagcgtgg cgtcgaagaa ttccgcggcc cccggcatcg actcgtcgac
    11461 ggcatcgccc atcaggtacc ggctcgagtt cgcctgacga tccaggtcga tcagcagcgt
    11521 gcgctgcccc tggcgggccg cgatcgcggc cagattgcag gtgatggtgg acttgcccac
    11581 cccgcccttc tgattgaaaa cgacccgtcg catcccgaga ccccgattca tccgaaaaga
    11641 cgattgtgcg ccgcaacatg agccggtcgg tgcgactggc gggtacttcc cctcgacttc
    11701 cggcgttcgc cagcgcggtc tcggcataac gtcgaagact ggaccgacgc atgccatgcg
    11761 cgccgcaacc tgttccgccc tgggttccac acccgcgacc tgatgaccgc cctgtactcg
    11821 ctccccctgc acctgctcat cgcgggcggc gcgctggctg cgctgctgat ctcgttcctg
    11881 gcgttcttcc tgctgccggg cctgctgcac tggtggcggc ttcgtcgcat cgagcgcgag
    11941 atcggccggc tcgatccgcg cagcccgccg gccgagctgc gcaggatctt cgcccgcgac
    12001 agacgcctcg cacagctctg gaaggagttc gaggactcgc tgcatgtcca gcgcgaggag
    12061 cgaggcgggc aaccggcccc ggtggccgtg cgttcgacac tgcctgcgga gaccttcttc
    12121 aacggtccct gcgtcgtcga tgcccggctg cgcaccgagt tcttcaagca cctgcccggc
    12181 atctacacgg gcatcggcat catcggcacg ttcagcggcc tgatcgatgg actgcgccag
    12241 ttccaggtga gcgagaacgc ggcaaccgtg cgcagcagcc tcgaatcgct gatgcatgcg
    12301 gtgggtcagg ccttcctgat ctccgcgacc gcgatcgccg cggccatcgt cgtgacgttc
    12361 ctcgagaagc tgctgctgtc ggcgctctac gggcgcacgg aagacatcgc ccacgccatc
    12421 gacgcacgct tcgacgccgg cgcgggcgag gactatctgt cgcgcctggt ccaggcgacc
    12481 gaaggagcgg ccggccgcag cctggtgctc aaggatgcgc tggtggcgga gctagccggc
    12541 gtgctgcgcg aacagaccga tcgccagatc agcgggttgg cgagcgccat cacgaacagc
    12601 atcgagagga gcctggctgc acccttgcag aagatcgcca gtgcggtgca ggccagcacc
    12661 agcgaccaga gcgcgctgac aacgcagttg ctgcaggacg tgatgggccg cttcggagat
    12721 cgcctcgaca acgtgctggg cgggcaggtg tcgggcatca acgcgctgaa ccagcagacc
    12781 ggccagacca tgcaggaggc cgtgcaggcc gttcgggcgc tggtcgagca gatttcgcag
    12841 tccagccgcc agtcgaacga ctcgatgacc gcgaacctgg tggatgccac gaaggcgatg
    12901 cttgcggctc tgggcgagag ccagcgcgtg tcggtcgaag gccagcagcg gcgcgagctg
    12961 gcgctgaccg aacgatcgtc ggccgccatg aacgacatga ccgacctgct ggcccggacc
    13021 ctggacgaag tgcgattcgc gtcctcgcag atggccggca gcatcgacgc catgaaattc
    13081 atgacgacag ccgcactcga caggatgagt tccggcgccg cccagctgag caccgcctcg
    13141 cagaccttct cgacggccgg cgaccgggtc agcggcgtga tggaacaagc cgccctggtc
    13201 tcctcgcaga tgaccgaggc ttcgcgcctg atgctggcaa gtgccgccga catgcgcagc
    13261 gggctcgatg actaccgtgc ccatcgcgac gccgtgggtc atctggtgac cgagctgcgc
    13321 acgacggtcg aacacgcccg caccgaggcg acgctgactg cagacgtgct ggatcgcatc
    13381 aaggtctcga cagagcgcct gggacaggct caggagcacg ccgaccttta cctggccagt
    13441 gtcagcaagg tgctcggcga cgcgcatcag gcgtttgcca tcgccgtgaa gaagacgctc
    13501 gatctggcca acaccgagtt ccacagcaag ctgtcgacag cggtcggcct gctgtcgtcg
    13561 acggtcgtcg aactcgaagc cacgctgggc aacgtcacac cgcagaggca ctgagcgtgc
    13621 tgttcgcaca gcgtcaggcc agacgcgcgc cccgtgacga ggccgagaag cccttctgga
    13681 tctcgttcgc cgacctgatg acggcgctga tggtgctctt cctcgtcgtg atgggcgtgg
    13741 cgctgctcgc catcaccaag aacgtcaccg accgcgagca gcagcaggag cagcgccgca
    13801 aggacatcga agcggtattc gaccagctcg gcaagactgc cgcccgccac gaaggcgtcc
    13861 ggatcgatcg ggaccgtcac gtcatcgatt tcggcgaccg cgcgcggttc gccttcaaca
    13921 agtccacgct ggccgcagag caggagatcg tgctgcgccg attcgtgccc gagctcatcg
    13981 gcctggccga ctcccccctc ggccgcaagg tgatgaagcg catcgtcgtc gaaggctatg
    14041 ccgaccgcac gggctcgtat ctcagcaacc tcaacctcag cctgcagcgc agccagcgcg
    14101 tgctctgcgc gatgttcgcg acacgcggcg acagcctgct ggacgatcgg cagaaagcca
    14161 gcgtgcgaga cctgttcctg gtgggtggct actcgttcaa tgcgtcgaag gccagcgacg
    14221 aggaaagccg gcgcgtggaa atgcgcctgg agttcctggg catgggcgaa cagcgcgctg
    14281 cgccggaagc ggcaccgggg agcttcggcg aatgcgcgct gccttgaacc cggtcgacca
    14341 tctggcttcg ctgctcgcgc ccgcggttcg cctggctggc cgggcgatgc cgcagtcggg
    14401 agagatcgac accgtgatcg cggcgctgca acgccagatc aagtccggaa gcaggcgccc
    14461 gctgaacgcg gatctgcagc acgacgcggc acagcgcttc tggcgcagcc cgcgtctgca
    14521 gaccctgaag gatgctcgcc tggtggcatt cggactgggc ctgccggtgg gacccggcgg
    14581 cgcccgcctc atcgatgaca gggcgcggtt cgatgccgtg ctgcgaggag tcgaccagtg
    14641 gctgaacgac gcccgctggt tccgtcgctg ctaccagggt ctggtctgga gctacttcaa
    14701 ctgcgacatc gacacatcgc tccaccattc ggatgcacgg cacaactggc tgcgccttcg
    14761 cgactacctg agccagcgcg cgcccagaac ggtgtccccc gaaagcaatc cggactgggt
    14821 caagacggca atctgccaga ggcacctctt cggcgacgca gcctgcgagc gtcacgctgg
    14881 cgacctgctg cgcggccatc accaggaagt ccaatcgatc tgcgagcagc tcggcatcaa
    14941 cgggtcctcg tggttcctgc gcgggctcgt gctggcgcag gtgagcgcag ccacgaagct
    15001 cgaccacgac gagttcaggg cggcgctccc caggctgatc gagctgcttt ccgacacgcg
    15061 tgcactgagg gacgagggtc tcgcccttct gctaaatcgc taccagcagt tgccgcaggc
    15121 gccattgcat gaaggacttc gcgacgctgc cgccagctgg tggggcagcc cctggctggc
    15181 gacgaacgag tttcgctgga gtggcatgag cgatggtgtg cgacgcatgg tgtcggagtg
    15241 gatccagtgc gacctcatcg accggttctt tgccgcggca ggtgacggcc cgagcggaag
    15301 gcgcgccgcg ttctggaagc gctacgtgaa gtcgaatcgc ctgatcggat tcgcgctggg
    15361 cggcagcacc gaacaggctg agcagtcacg tgcccggctt ggagtacagc gagtgaccgg
    15421 gccgtgcgcc agcctggtcg atggttcgct cacgcatagc gccctcgtcc tgacgatggg
    15481 ccgcgccgtc atcgttgaat tcggcgacct gtcggtgccg ctgcatggct acgacctgcg
    15541 ccattcgcag ccgttcgacc tgtcctgccc catgtcgctc gaagaggatg ccggaaactc
    15601 gttacgccag cgtcaccgca gcctgctcct gccgcaccag gatggtctgg aaggctggcg
    15661 ccggtgggag cagatgttcg aggcggccat cagcgatcag ttcggcgtgc gtccaggcac
    15721 cgcgccttcg acggatgaga cttcgtgcgt ggatctgtcc gacccggcgg accgcatcga
    15781 ccgcggcgac cccgaaccgc tggggcagat cgatgagcca cgctggcaga cctcgtcgtc
    15841 cggcgaggac gcgcactggt tgaccgccga ggccgccagc gtgccctaca gccgcgccga
    15901 cctcgaggtg ctggcccggg tccacgcatt gcgcatcgag gacgattcct cgcgcagcgg
    15961 cagactctgg gtgcgcaccg accagggcga ccaccgcatc gcacgggtgc tcaccggttg
    16021 gggcttcgtc caccggccgg gcgaaggatg gctcaggtag gaccctgagc cgtcggatcg
    16081 actcccgcga gctcaggctt cgctggtgcg gcggatgcct tcctcgagca cgaagaactg
    16141 cgggttgagg cccgccatcg gcgcacgcag cttctcgatc tcggcagcgg gtgcgtgcac
    16201 ttcaagacgc acgagcttgg cgagcttcag cgcttcgccg agcagttcgc ccacgttctg
    16261 caggtgcgca agcacggcgt cggcattgtc gtaaccctcg cggcaatggg ccgtgccgcc
    16321 actgaacgaa aaggcgtagt ggcgacagcc tgattcggta cgcgtcttcg caacgaactg
    16381 ggggccgagg gccttgaacg cggcgagttg accgtccggc acctcgaagt agggaaccag
    16441 ggtgcagcag gtatcgagtg tgaacatcgg tgtctccaga ttgtcaggtt cggatcgagc
    16501 ttcttgcgcc gatcgaccgc cgtggcggca gcgcaagtac atggaagacc tcggttgcga
    16561 ccgggatcct ctggctggga gtgtcagcgc tgcatgtcac tgcgcaggct gggtcgatcc
    16621 ctgacgaccg cgggccttga tcgactgggt gatgcgctcg tccatcgccg cgaactccga
    16681 cagcagcttg ctgcattccg cctggggcgc ctcgcacagg ctggccgttt cgaccgacac
    16741 cgagccagcg gacgagcgcc ggatgatccg gcgcacgaca gacgggtagg cgtgatgtcc
    16801 gtcgggagcg aaggaccatt gagcggaagc gagcggctcg ttgatgatgg tccagccgtc
    16861 ggcatggacg accaccgtgc cattgccgtc ccgcgcctgg agctccttga gcgccacggc
    16921 aaccgacgga aattcgatcc gcgagggctc tggtggctgg gtctgtgcca gagcgggcaa
    16981 cgaggaagct atcgaaagga caaacagggc aaggcgcatc cctgcagtgt atttggcaag
    17041 ggatgcagcg gccgcgctac tgagtttcag ccgctggagt gcaagccgcc gagccgcaac
    17101 ccgagatcgc ggtctgcggc aacgcctggg gctgcgccac gctgccgctc gatacgtcgt
    17161 agcccaccga cttcaggtgg tcggccagtg ccgcatccat gtttcgcgca tgctcgggaa
    17221 accacatcgc cagctcgcgc gccatggtcc ggatgatgtc catccgcttg agctgcgcgc
    17281 cttcattcat gaccttcatc accatggcgt gctgcgcact gtggcagttg ggcatcgaga
    17341 agcccgtgtc ctgcatccag cggtcttcgc gcgcgaaatg gatgtcggtg tgctcgatca
    17401 gttcgtccca agccccgaac agctgatcgt cgggcgcctg ctccaccgcc gcgagcagtt
    17461 ccacgaattc gcggtgggtg cggtccatga ccggcatatc cagcgacagc tcaggtgccc
    17521 attccagagt actcaacacg ccctccatgc cgcgccgatg cggcagacgg gcagaggcta
    17581 gctcggcgag gccgtgtgcc tcttgatgcg cgtcaagcag caccggtcac ggcgcccgct
    17641 cgcgaccctt cagctgcgca ccgcgcccac cagctgtctc gtgatcgctg ccttgatcag
    17701 gccgctgaaa ggcggcacct tctcggccag tgtgagcacg gcccggcggg ccagcttcgg
    17761 cagcgcacgc tcgtcgcaga acagcgagac gatggcgttg gtgcccagat agaccggcag
    17821 cgtggtgcgc cggtgttcgc gctcgtaagc ctgcaaggcc ggcagcgcgc cgatgtccct
    17881 gcccgcggcc agcgaggccg ccagttcgcg cgccagcact tccacgccgt agagaccgaa
    17941 gttccagcca tgcgccgtga ccggatgcat gccgaccgcg gcatcgccca tcaaggccaa
    18001 gcgcgctgcg acgaagcggt gcgcatagac ccccaccagc gggtagagat ggcggggccc
    18061 ggcggtcgcg gtcacgccga ggcgccgatc cgactgcgcg tcgacgcgcg ctgcgaactc
    18121 ggcgtccgca agtgccatcc agtgcggcgc ctgatcgctc gccacggtca ccacagcgga
    18181 cgattggccc ttgttcagcg gcagcagggc cagcgtgttg ccgtagcgga agcattccca
    18241 ggcgactccg ccgttgtcct gttcgtgcgc gacccgaccc acgaccacgc tgcggccgaa
    18301 atcgtgcatc gatgcgccga tgccggccag acgccgcatc tgcgagaacc ggctgtccgc
    18361 agcgacgacc agcaacgcgt tcaatcgccg gccgtccgac agcgtgaccc cggtctgatc
    18421 ggcggtggga tgcaatgccg tgacccgggc gtcgcacacg cagcgcacac cctgtcgctc
    18481 ggctgccgcg gcccacgccg cacgccggat caggtgattg ggcaccagcc agcccagcgc
    18541 cgaaccatcc acccccgagg actcgaagcg cagcacggtg ggggcatcgc catcgacgac
    18601 gcgcgcctcg cgcagcggag cgatctcgtc ggcgggcaga tgccgccaca gcccgagttg
    18661 ctccatcacg cggcgggcgc ggtgagtcag cgcaatctcc cggccatcct cgggagggtc
    18721 cacgagcgca ccttcgggct gctgctccag cagcagcacc gagagcccga tgtcgccgag
    18781 cgcgcaggcc agcgtcaggc cggcggggcc ggctccgacg atcagtacat ccgcatcgcg
    18841 ggcaggcagg gaatgggaag gcgtcatcgc cgcgagcatg ccctgcgaca aggccgcggc
    18901 acttgacggg aatcagacct gccgcacctc gggtggtcga acggcctcgg cgcaggcccc
    18961 gatccgatgc cgtcagacgt ggagggcgtt ccccaggtcc tgctccagca gcctctggtg
    19021 gagcgcatcg agccgttcgc cggggcgctg ctcccgggac aggcgcagcc ccaggctctg
    19081 accctggaac ttcatccagc ctcgggcgcg ctccgtgagg ccggtgaccc tggcgatctt
    19141 cctgccgctg tctgccgggt cgctgacgaa ctcgacccgg aactcgccac aggtcccggg
    19201 cgtgagatcg gcaacggggg ccggcatggt cccggcgaag ccggcatcct cttccaacag
    19261 gtcctgcagt atttcctcga gcggctgccc ttccctggcg caggcatcgg cccgtgcaag
    19321 taccgcgggc atgccgtgct gctcgagcca ggccttggca cgctcgctcg tgggcagcag
    19381 ccgggctgtc tgcgtggtct catcctcttc gacccactcg agcgagaact cgggctttcg
    19441 gctgatcgca ctcatctcat ttctttcctg gtgccctgag gtccgccgtc ccgcacattc
    19501 aagcggacag cttcctgacc gcatcgatcg cggccaggca ggcgtcgtgg cagtccttgc
    19561 attcggcatg gtgctcgacg tgcggcatgc aggccttcac gcaggcttcc atggccttga
    19621 tcgaggtctt cgccagtgaa ggcgtgtagc tcgagttgat caaggccgcg cgcagggtgg
    19681 cgttgcagac gacgtcgcaa tcgagcgcag tccgcaggca gcgccccaat gagcggtcac
    19741 cggcggcgag caaggcctgg caatgcgaga tgcagatcgc aaccgccttg ctgcaggcct
    19801 ggaaaggctc gacgagatcg tcgtagcgtc gaggcgcggc agctcctcgc gcggccatca
    19861 tggccttgtg atcgtgctga tgtggttcct gggcctgtgc ccggcccgcc gtaacggcgc
    19921 ccagaccggc agcggcgaga ctggtgatgg aaccgatgaa actgcgtcgt tgcatgacgt
    19981 gcctttccgg aaaatggcct gtgcgttgcg gatgtttcag ccgcgccccg gagtttaggc
    20041 cgggcatgcg cgaccacctg cctccgatgc gcaggacggc tgctcagaag aagccgcggg
    20101 gtatgagccg gtgttcgtga gcgctgcgct cgagttcctc gcgaaagtcg ggatgcgcga
    20161 tcgagatgag cgccctcgcc cgctccgcca ccgacttgcc cttcaggttc gtcatgccgt
    20221 actcggtggc cacgtacatc atgtccgagc gcggcgtcgt gacgatgttg ccgctggtca
    20281 gcgacaacac gatgcggctg cgcctggcac cgcgcttctc gtatgtagag gcgaggcaga
    20341 cgaacgattt gccgccgttc gatgcgtagg caccgcgcac gaactgcaac tgccctccgg
    20401 tgccgctgat gtgtcggtgg ccgtcggact cggacgcggc ctgcccctgc agatcgatct
    20461 gcgtggtgtt gttgatcgcg atcacgcggt cgttgcgcat gatgttgtgc ggcagattgg
    20521 tgtagtcgac ggggtagaaa tgaatgtcgg gattgcggtc gatcccgtcg tagaggcgac
    20581 gcgaaccgag cgcgaaggtg cagacgatct tgccgggatt gagttgcttg cgcgcgccgg
    20641 tgatgcggcc ggcctggtac agatccatga tgccgtcggt gagcatctcg gtgtggatgc
    20701 cgaggtcatg aacgccgctt ccgctcagca ggctgcacac cgcgttgggc atgccaccga
    20761 tgccgatctg caggcagtca ccgtcttcga tctcgcccgc gatcagccgc gccacggcct
    20821 cgtccacctc cgtgggcggc gggttcggca actcagtggc gggcgcggaa tcgccctcga
    20881 tgatgtagtc gacctcgctc acatgaacgc cggtctgctc gcccagcacg taaggaagac
    20941 cgctcgatac ctcgacgatg accatccgtg cccgctcgat cacggcgcga tgccagaccg
    21001 tggcggcact gaaattgaag tagccgtcgg cgtccatcgg gcacgtcttg atgatgacga
    21061 tgtccaccgg gtcgatgaag cgccggtagt agtccgggat ctcgcccaga ttgaccggca
    21121 ggtagttcgc gatacccgca tcgtgcttgc ggcggtcgta gccggagaaa tgcaggttga
    21181 tccagaagaa gtgttcaccc tgcggatcgg cctcgagcac ggcacggggg cgcagcgtca
    21241 ggcacgcccg aatcttgacg ccatgcaatt cgtcggtgcg ccgggcaagc gccgcgtcga
    21301 agacatcggg ctgacacagc gacgcgccgt aatcgatcca catgcccgac gcgacgaagc
    21361 cggcggcctc ctgcggcgag atgttgcgtg ccttcgaggc ggcgattctt gttttcatgg
    21421 gctcatctga cggggctggt tgatccggcg gctcggcagg atcgaaccgc gcggcaaacc
    21481 agaactgccc ggtcgaaccc cttgcccggc atcgacacca cggacagtga ccgtcaccac
    21541 tgtgcggcca tcgcgaaggc cgagccagcc gcattctcgg accagtttcc accccgatcg
    21601 gcacttgaaa gccccgggtg gacgccgagc ggacggcccc gtatgctgtg ccgcccccac
    21661 gacaccccct cttccatgac cgcagccgta ccccgccgtg tcggcctcta tgtggcggtg
    21721 gtgcagttct tcttcgcgct gacctggtcg gtctacgtga tctacctgcc gcaactcgcc
    21781 gcgcaggcgg gcatccccaa gacggcggtg atctggctgc tgatgctcga ccagctggtc
    21841 ttcctggtgg ccgactacgc ctgcggggtc gcctccgatc gcatggcacg gctgaacagc
    21901 cgcatcggcc cggcggtact cgcggtcacg gtgctgtcgt gtgcggcctt cctgctgctg
    21961 cccttcattg ctccccgggg atcggccctg ctgttcattg cggtgaccgt gctgtggact
    22021 gtgggctcgt cggccctgcg tgcaccgccg ctgaacatca tcgggcgtca tgccgcccgg
    22081 ccgagccaac ccgcgctggt cgcgctgtcg atgctgggcc tcggactggc cgccgcggcg
    22141 gcgccctacc tggcaacggc gctgcgcgag gtggacccgc gctggccgtt cgcgctcgcg
    22201 agcatctcgc tggctgccac caccctgggc atcatcgcgg ccgaacgggc gctgcagggc
    22261 cggccggcgg ccagcgtcac gtccgcaccc gacgcgctgg tgaagtccga cccgcggcgc
    22321 ctgccgtggg cgccctgcct cgtcgcggcg gtgctggcgg cgatcgcctt ccaggtgcat
    22381 gtcttcctga acagtacgcc gctgtacctg cggcatgcga attcgggcga cattccgcag
    22441 ctggccccgg tgttctggat cggtttcaac ctcggactct ggccggcctc gctggcgatc
    22501 aagcgccacg gaggcatcgc ggtgacggcc gcagccggcc tcaccgccgc cgtcgcgtcg
    22561 gcgtgggccg cgggcgccgg ctcgatcgaa acgctggtgg ccgcgcaggc gctcgccgga
    22621 gcggcctggg cggcggtgct gacgagcgcg ttctccgccg ctctggcgct cggccacacc
    22681 ggcgccgaag gccgcttcag cggcgcgctg tcatcgacgc tggcactcgc ggcgctgctg
    22741 cgcatgggcc tgctcgcgtc gggtctgacc agtctgccgc acgacagcac gatgcgcctc
    22801 gcgctcgact ggcttcctgc tgccctgtgg ctgctcgcgg gcgccctgct gctggcgatc
    22861 tggcagcgag aggccgccag gacccgattc aggccatgac gcccaggccg ggccgttggt
    22921 gccgagcagc gagaatcgac gccctggcct gaccggtgtg aatgaggcga aacacaggat
    22981 gcggttcacg agagtctccg acgtgcatgg gccgggccac ggtgagtggc agtcctgtcc
    23041 gcacgccata cacccggtgc gagtagccca cctgccccgc atcggcggcg aggtcgggac
    23101 agcgacgccc cagaccagcg ggcgaaccag cgcatgagcg ctgaccgtcg gcggctgacg
    23161 ttcgcgctgc tgttctccct gctgattcat gcgctgctgc tgagcctcac ctttggtggg
    23221 cagggcctcg gcctgccggg cttcggcttt ccctgggagg agcgacgggt cgaggtaccc
    23281 gatctgcgca tcgtgatcct gccgacgccg gtcacggccg ccgagccggc gatcagttcg
    23341 gttgacgagt cgcggctgca ggcatcgatc gagcaggtgg ttgccggtga gcccacccag
    23401 acagcctccc cgcctccagc gccggcatcg gaaaagacgg cggatgcggt cccgcccaag
    23461 acgcggcgcg cggcagcagc caagccgaag gcagagacca gtccggaacc agatcccacg
    23521 accggagcgg ccgccgatga agccccagtg cccgccgagg agcccgggga agtcgcaccg
    23581 gccgcgatcc ccgatgcgcc ggtgatcgcc gtggatagac ctgacgaggc cacgtttgtc
    23641 gtgtctgccg ctgcctccga gcccacaccc gtgatcgccg cagcgccagc cgcatcgagc
    23701 ccggagaccg ccttgcccaa gcctcgggct gccggcgatg ccgtgcggga acgggtcgac
    23761 ccgaatgcac tggagcgcgc cgtggaactc gccagggtcg agcgttccga gcaggaggcg
    23821 gcccggcagg aagctcaacg gatcgagatc gcacggctgg aggctgagcg acaggannnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg gcaggaagct caacggatcg
    24001 agatcgcacg gctggaggct gagcgacagg aggccgttcg gcaagcggca gcgcgccagg
    24061 aggctgcccg gctggaggcc cagcgcatcg agaccgctcg actggacgcc gagcgacagg
    24121 aggctgcccg gcaagcggcg gcgcgccagg atgcgtcacg ccaggaagct cagcgcatcg
    24181 aggccgctcg gctggacgct gagcgacagg agactgcccg gcaggcggca gcgcgccagg
    24241 aggcatcacg gcaggaagcc cagcgcatcg aggccgcgcg gctggaggcc gagcgacagg
    24301 aagtcgctcg tcaagcagcg gcgcgccagg aggcggctcg gcaggaagcc cagcgcatcg
    24361 agaccgctcg actggacgcc gagcgacagg aggctgcccg gcaagcggcg gcgcgccagg
    24421 atgcgtcacg ccaggaagct cagcgcatcg aggccgctcg gctggacgct gagcgacagg
    24481 agactgcccg gcaggcggca gcgcgccagg aggcatcacg gcaggaagcc cagcgcatcg
    24541 aggccgcgcg gctggaggcc gagcgacagg aagtcgctcg tcaagcagcg gcgcgccagg
    24601 aggcggctcg gcaggaagcg cagcgcatcg aggccgcgcg gctggaggcc gaacgccagg
    24661 aagccgctcg gcaggcggca gcgcgccagg aggctgcacg gcaggaagcg cagcgcatcg
    24721 aggccgctcg gctggatgcc gaacgacagg agactgcccg gcaggcagcg gcgcgccagg
    24781 aggcggctcg gcaggaagcg cagcgcgtcg aggccgcacg actggaggcc gagcgacagg
    24841 aagctgctcg gcaggcggca gcgcgccagg aggcggctcg gcaggaggcc cagcgcatcg
    24901 agaacgctcg gctggacgcc gaacggcagg aagccgcccg gctagaggcc gcccgacaag
    24961 cagtggcacg tcaggaggcg gcgaagcggg aggccgcact cgaggacgat gcgcgacgcg
    25021 aagcttctcg ccgcgcgatg ggccgccagc tggatgagga agccgcccgg cgcgaagcgg
    25081 cttcggcttc gacggcagcg ggtatgccgt ccaggctccc accctcgtcg agtggcgcgc
    25141 gtcgcggcag gctgttcggg cgcatcgacc ccaacgccga actcgtcctg tacgcggaag
    25201 cctgggcccg caagatccag ctgaacatga ccttcgacat ggtccgcgag gtggtgaagc
    25261 ggcctcatgc cgatccgctc gtcacggtgg cgattcgcag cgacgggtcc gtggaatcgg
    25321 tgacattcgt cctgtccagc ggcgtcccgg aaatcgacga agccatacgc ggcatcctgc
    25381 aaggccaggc gccttaccag ccgttccagc cggcgctggc gcgcgagttc gacgtgatcg
    25441 agatccgcag gtcctggcac ttcgacatgg ccatccggct gtactgatcg caggctgagg
    25501 ccgtgtccgg cgaggcacgg cgacgggtca gatgagcgtg acgttcggcc tgcgatcggc
    25561 ccacggttgg cagcgttcca gcgctgcagc ggcgcggaag atgtcggttt cgcggtgggg
    25621 gcgggacacc atctgcagcc ccaccggcag gcccgcccgc gtccagcccg ccggcaccga
    25681 ggcagccggc tggccggtca ggttgaaggg gtacgtgtag tagacccacg agatcacgtt
    25741 gcggtcgggc cattgcggtg gcgtgttcag tccgacgtcg aaggccgcgc agggcagcgt
    25801 cggcgtgagc agcaggtcga atcgttcgaa gaaccggcgc atcgtctcgc gcagttcgta
    25861 gcgctggaac acgcgcgtgc agtaggtctc cgtggtctgt tccaacgccc ggccgacgat
    25921 gtcggcgacc gccgggtcga gcagttcgag ctgttcgatc agcgacttct tcaggcgcac
    25981 cccgacaccg gcatagaact cggaggccca caggtcgatc gggtcctgcg ccagcacgcg
    26041 atccacctcg accacctcgc aacccatctc gctgaaccgc tgcgccgcgc gctgcgcgat
    26101 gtcggcgatt tcaggatcgg gcctggcata gccgagggtg cggctccagg cgatgcgcag
    26161 gccttcgaca ggctgatcgc aggcgcccag gtagtccggc acggggccgg actacctggg
    26221 cgcctgcgat cagcctgtcg a
//
LOCUS       DAOORV010000041        26166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2234_length_26166_cov_8.946881, whole genome shotgun sequence.
ACCESSION   DAOORV010000041 DAOORV010000000
VERSION     DAOORV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_26166_cov_8.946881"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1934
                     /locus_tag="PKV56_07570"
     CDS             261..1934
                     /locus_tag="PKV56_07570"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOX67712.1"
                     /translation="MDKPWLKHYPPGVPARIDVDAFPSLVAMLDDSFSRFRDRAAYRF
                     MGKSITFDDVDQASKAFAAYLQAQGFAKGDRIALMMPNVPQYPVAVAGVLRAGCVVVN
                     VNPLYTPRELEHQLKDSGATCVVIVENFATTLQKVLPTVPIRRVIVTSLGDLLGVPKS
                     WIVNYVVRHVKKLVPPFELPGAVSFRAALSAGRNLPMQPVAIGPDDIAVLQYTGGTTG
                     ISKGAVLLHRNLVANVLQSEAWHGPALKKLPPGEQLVTVCALPIYHIFGFNTNMMLGL
                     RMGGCSLLIVNPRDLPAMFNELAAERFHSFPAVSTLFKAMASHPDFGKVDWSRLIISV
                     GGGMAVQSATAQLWLQKTGCPIVEGYGLSETSPTASCNPIDSTSFNGTIGLPVSNTEM
                     KLLDDAGEEVTMGSPGEIAIRGPQVMAGYWQRPDETAKVMTPDGFFRSGDIGTVDERG
                     YFKIIDRKKDMILVNGFNVYPNEVEEVVSQLAGIAECAAVGLADEKAGEVVRLVIVRS
                     DPQLTEGAVKAHCEALLAGYKRPRVIEFRADLPKTNVGKILRRELRDRR"
     gene            1965..2696
                     /locus_tag="PKV56_07575"
     CDS             1965..2696
                     /locus_tag="PKV56_07575"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOX67713.1"
                     /translation="MAPRFFVPPPLRAGDVRALPPGPTRHVQVLRLQPGDDITLFDGG
                     GGEWRARIDRIGRSDVSARLIEHVETSLELPFDVTLALGMPANDRMDSVVEKATELGV
                     AGIQPLVCERSVLRLTGERADKKVAHWQAVAASASEQCGRTRVPTVHPVRTLPQWLAS
                     LTSDATDRRLVLSLHETRPWSVVAAQDQRLLFLSGPEGGLSEAEEQAALQAGFEPTSL
                     GPRVLRADTAPLAVLAALALQFTPN"
     gene            2720..3259
                     /locus_tag="PKV56_07580"
     CDS             2720..3259
                     /locus_tag="PKV56_07580"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HOX67714.1"
                     /translation="MSSIGHQNQENSLTEIAMGLLDSVIGALAGGTQGNTAGAGGGQA
                     ALISIVLGMLTQRGGGLGAEGAEGGVSGMGGLGDLLARFQQAGLGDAAASWVGTGANQ
                     PVSPDQVEGALGSDLLSRIARQIGMPQGETAGQLSELLPQVVDRMTPNGRLPESGFNE
                     DDIAGFEGLDDLLARFSKP"
     gene            complement(3282..4535)
                     /locus_tag="PKV56_07585"
     CDS             complement(3282..4535)
                     /locus_tag="PKV56_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOX67715.1"
                     /translation="MSAPFFLPCAAGVEALLATEVQRVLPDTAVHTLRGGVALDGGPH
                     EVMVLNLESRLAQRVLVEVAEGPYRDEQALYDLAASVDWMQWITPEHTLRVDTTAHRS
                     PLRSLNFAALRVKDAVCDRLRDGTGERPSVDVRNPDLALVLHLGEERAALYVDTSGEP
                     LFKRGWREDKGDAPLKETLAAAMLAAAGWQGRPDAGGALHDPCCGSGTIAIEAAQIAC
                     GIAPGLKRRFAFERLLPFATPEMRAELQRLRSHAQSRIHASAVPIHASDVSFRMVDFA
                     RRNAQRAGVEAAITFNGGDALERPAPDLPDDLPGTLMLNPPYGERIEVAGKAARGAMP
                     ADGPGNKELPDDFFARLSAHWKRAYTRHPAGWTAYVLSPDMKLPGSMRLKESRRTPMW
                     NGPIECRLFRFDLVAGTMRGDAALR"
     gene            complement(4544..6082)
                     /locus_tag="PKV56_07590"
     CDS             complement(4544..6082)
                     /locus_tag="PKV56_07590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOX67716.1"
                     /translation="MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLDQN
                     PFLETEDEGLPAFEPMVERSTPTERVAERETERADTPSESSNEGGDDAPGVDAAEFGT
                     TERDDWENGTEREDFDGIRETPGKAGTGSDGEDFDPQDRNPTPASLQDHLRTQLLGMR
                     LSPEDAAAVEVLIESLDGDGYLADSLEEIAASLAGDDEDRLEEVMERLSCALKWLQNM
                     EPIGVGARDLPECLTLQLRLLPRSEAQMIAIMICKQHLELLARRDLKKLMAVTGADED
                     LVKEAQSLITALEPKPGRPFARAEANIIVPDVIVQKSGRNWKVVLNPDVMPKLRINDL
                     YAQAIRQGRGAANANGSHAGLSSRLQEARWFMKNIQQRFDTIQRVSQAIVERQKNFFT
                     HGEIAMKPLVLREIADELGLHESTISRVTTAKYMATVFGTFELKYFFGSSLNTDAGGN
                     ASSTAVRALIKQLVAAENVLKPLSDSQLSKMLEEQGIQVARRTVAKYREALRIAPANL
                     RKTM"
     gene            complement(6198..6971)
                     /gene="lptB"
                     /locus_tag="PKV56_07595"
     CDS             complement(6198..6971)
                     /gene="lptB"
                     /locus_tag="PKV56_07595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOX67717.1"
                     /translation="MNTAAAEARPAVHRLSATGLQKSYGARKVVKDVHLSVDAGEVIG
                     LLGPNGAGKTTSFYMIVGLVPADAGEITIEGQRVERLPIHKRSRLGLSYLPQEASIFR
                     KLNVEENIRAVLELQLDANGKTLRPAVIDELLEGLLRDLGIEKLRDSPALALSGGERR
                     RVEIARALATQPRFILLDEPFAGVDPIAVIEIQRIIGFLKTRGIGVLITDHNVRETLG
                     ICDRAYIISEGRVLAEGTSSEIVDNPDVRKVYLGENFRM"
     gene            complement(6968..7618)
                     /gene="lptA"
                     /locus_tag="PKV56_07600"
     CDS             complement(6968..7618)
                     /gene="lptA"
                     /locus_tag="PKV56_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOX67718.1"
                     /translation="MNPLTSASPLPWSTRLRLIVVVAVAAIGAPFVHAEKADKSKPLN
                     IEADNGRYDDAKQMGTFTGNVVVTKGTMSMRAAKIEVRQTPDGAQYGVATADADKRAV
                     FRQKREGLDEYIEGEGERIEYDGKADTIRFVNRAVIRRFRGSTLADETAGSVIVYDNT
                     AEVFSVVGGAAAATPTNPSGRVRAVLTPREVTPAAEAASSAGGVTLKPSTALGSKR"
     gene            complement(7740..8393)
                     /locus_tag="PKV56_07605"
     CDS             complement(7740..8393)
                     /locus_tag="PKV56_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HOX67719.1"
                     /translation="MKRREFSTQLLATVAGATVGSAVFSAHAQGDFVEGQHYVKLSQP
                     QAVPGGGKIEVVEFFWYGCPHCNAFEPALEAWQKKLPADVAFRRIPVAFREEPFTTHQ
                     RLFFAIETMGLVDTLHRKVFNAVHLDRARLDKLPELVEFMNKNGVDGAKFSEVYNSFS
                     VQTKARQAKQLAEAYRIDGVPAIGIQGRYYTSGSMAGSGERSLAVADYLLNRVRKNT"
     gene            complement(8537..9178)
                     /locus_tag="PKV56_07610"
     CDS             complement(8537..9178)
                     /locus_tag="PKV56_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX67720.1"
                     /translation="MRTRARPRHSLSLQRGGFFVGVIVGLLIGLALSLGVALYVTKVP
                     VPFLDKVPQRTEEQDAAEREKNKNWDPNAPLAGKNPARPASSVAAASAPASGAPPAGS
                     GRDPASILADRATSGPEPAGKASTPVSTGSADEAFSYFVQTGAYASAEDAESQRGKLA
                     LLGYSARVTEREQSGRTVHRVRLGPFEEKVEADGTKEKLLAGGFEAVLVRAPK"
     gene            complement(9187..10899)
                     /gene="argS"
                     /locus_tag="PKV56_07615"
     CDS             complement(9187..10899)
                     /gene="argS"
                     /locus_tag="PKV56_07615"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOX67721.1"
                     /translation="MIEAKQDLLKALASAIEEVSPASPLKAAFESPKLAAHGDLACTS
                     AMQLAKPLKKNPREVAQALIAALERQPAVQRHVESFEIAGPGFINLRLRASARQAVVR
                     EVLAAGACFGRQAANAQHVLVEFVSANPTGPLHVGHGRQAALGDAICNLFETQGWDVR
                     REFYYNDAGVQIATLAASTQARLRGYQPGDAHWPESAYNGDYIADIAADFTARKTVKA
                     DDREFTASGDVNDLDGIRQFAVAYLRHEQDLDLQAFGVRFDHYFLESSLYANGQVEAT
                     VARLVAAGKTYEQDGALWLRSTDYGDDKDRVMRKSPAPGASADGEAQYTYFVPDVAYH
                     ISKFQRGYTKAINIQGGDHHGTIARVRAGLQAAGVGIAPGYPDYVLHKMVTVMKGGEE
                     VKISKRAGSYVTLRDLIDWTSRDAVRFFLISRKADTEFTFDVDLALKQNDENPVFYVQ
                     YAHARICSVIQQYVDKGGDPDAIGRADVSVLTAPTETALMLKLADYPAMLTSAAESLA
                     PHDVTFYLRDVASAFHSYYGAERFLVDDDAALTQARLALLSATRQVIRNALTVLGVSA
                     PEKM"
     gene            11174..12484
                     /locus_tag="PKV56_07620"
     CDS             11174..12484
                     /locus_tag="PKV56_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOX67722.1"
                     /translation="MPEQIGKYPVLRKLGEGATSEVYLCRDEFQARDVAIKRVRAAST
                     SDAVANRYSERFFAAEAALVGRLQHPNVVKIYDAVSDASSQYLVMEYVAGTTLRPYCR
                     ADQLLPLELIVEIGFKCAMALGYVYRQGLIHRDVKPANVLTVLTNGAITDVKISDFGS
                     VLNLDSDVTQVYRVGSLAYMSPEQLDGSSLDCRADMYSLGAVLYHLIAGRPPFDAQIQ
                     SAMMHQIYHATPASLVGMRDGVGVAVDSVIQRALAKHPQDRYPDWDAFAQALSGLISN
                     QEVPRGQLQGVLDSERFNMLRKLEFFASFGDVELWEVVHRAKWQRFPVGHALYRKGEE
                     GRTFHIIAQGELEVFREGNKVAVLGAGTSVGEMAYLAPSPDLRRHSTDVLISEQATTI
                     SFVPETLAQVSPNCRHLFDDAFIRVLVRRLHAAHEALAHPRRIL"
     gene            12634..12951
                     /locus_tag="PKV56_07625"
     CDS             12634..12951
                     /locus_tag="PKV56_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HOX67723.1"
                     /translation="MKNLMIALIVCTAALSGTAYANSHKEAPEMAAAASAPEMGKQQS
                     KMTTCNKDAGEMKGDERKAFMKKCLSAKKPTQQEKMKTCNAEAKGKTGEERKDFMKSC
                     LSS"
     gene            complement(12972..13892)
                     /locus_tag="PKV56_07630"
     CDS             complement(12972..13892)
                     /locus_tag="PKV56_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX67724.1"
                     /translation="MDKLKQIESFTQVATKGSLTAAALVEGVAPAVIGRRIDALESRL
                     GVKLMVRTTRRITLTHEGSAFLEDCQRLLADLNNAEASVSAGGVKASGYIRVTAPAGF
                     GRRHVATLVPRFIGLHPDVNVSLSLSDRIVDIVNEGVDCAVRVGDLPDSSLVSVRLAD
                     NRRLCVASPAYLQRAGIPTQPSDLYQHECLTFSSDASQPRGWAFTLEGVTTHLRPSGR
                     VDCSDGQVLHEWCLAGLGIAWRSMWEVEQDVASGRLVSVLDEFASPPNGIYAVFPQRK
                     HLPLRVRLWIDFLKHTYGDATYWRAASPQG"
     gene            13994..14698
                     /locus_tag="PKV56_07635"
     CDS             13994..14698
                     /locus_tag="PKV56_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HOX67725.1"
                     /translation="MSAPALRPRPLAVLFDAYGTLFDVYSVATLAEQLFPGQGERLAT
                     LWRDKQIEYTRLTSMSSSRGEHYRPFWELTRAGLKYATARLGLALDTATEERLMNEYR
                     HLGAFPENHEVLTALKSRGIPAGVLSNGDPDMLGVAVKSAGFAALLSPVLSAHPLRKY
                     KTDPAVYALGTQAFELPGKDILFVSSNGWDAMGASWYGYTTLWVNRAGMPPETLGPEP
                     TRTGTSLRDVLRFFEP"
     gene            14710..16317
                     /gene="aceB"
                     /locus_tag="PKV56_07640"
     CDS             14710..16317
                     /gene="aceB"
                     /locus_tag="PKV56_07640"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOX67726.1"
                     /translation="MSQTSSSAAGTLSLPAGMQINAPILPGFESILTLPALELVAKLH
                     RSFEPTRQKLLAARVVRAQRLDAGERPDFLAETKHIREGDWKIATLPTALHRRRVEIT
                     GPVDAKMVINAFNSGADSYMTDFEDSNSPLWTNQIQGQINLGQAIRRTLTLDANGKHY
                     KLNDQIATLQVRPRGWHLDEKHVLVDGQRVHGGIFDFALFMFHNAKELLARGAGPYFY
                     LPKMESHLEARLWNDVFVMTQKELGLPQGTIKATVLIETILAAFEMEEILYELREHSA
                     GLNAGRWDYIFSCIKKFKLDRDFCLADRAKVTMTAPFMRAYALLLLKTCHKRGAPAIG
                     GMSALIPIKNDPAKNEVAMAGIIADKRRDATDGYDGGWVAHPGLVEASMKEFVAVLGD
                     RPNQFDKQKPEVQVAAADLLNFQPEAPITEAGLRMNINVGIHYLGAWLAGNGCVPIHN
                     LMEDAATAEISRSQVWQWIRTPKGVLDDGRKVTADLVRSLVPEELAKIKAGGFEGKFD
                     RAAGIFEEMSTQDAFAEFLTLPLYEEL"
     gene            16393..17019
                     /locus_tag="PKV56_07645"
     CDS             16393..17019
                     /locus_tag="PKV56_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOX67727.1"
                     /translation="MPHLLHIESSPRKQRSASIEVARAFIDTWCARHPQGSVDTLDVW
                     ATALPEFDSAALDAKYAGLEGRTRSDDEARAWSGIEALGQRFHRADVIVFSVPMWNFG
                     IPYRLKHLIDAATQKDTLFTFDERGLLGLLGGRSMVLVAARGVALGDDFPAAIYDHQV
                     SYMSMWARMVGITEVHHVTVEKTLFGPEADTASREQAKAQAQALAQSL"
     gene            complement(17057..17932)
                     /locus_tag="PKV56_07650"
     CDS             complement(17057..17932)
                     /locus_tag="PKV56_07650"
                     /inference="COORDINATES: protein motif:HMM:NF022457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HOX67728.1"
                     /translation="MTDIISLPIASWDQPSSQDEQLAAIRSLEDGNVLLLPQLDFPLL
                     DVERQFLSPDISGAAKNVSLNPSDGSVRGSSAAGAELAALHGMMERYASSSLTLMRQL
                     LSPYASTVQQGRTSFRPVEVAGRETSWRKDDTRLHVDSFPSSPTGGRRILRLFTNVNP
                     QCRTRTWRLGESFEGVARRYLPSLHRPLPGSCELLQLLRITKGRRTAYDHFMLQLHDR
                     MKADTKYQAEVEQRTHEFPPGQTWLVFTDQASHAAMAGQYLFEQTFYLPVAGMVDPAK
                     SPLRVLERLMRRELA"
     gene            complement(17997..19112)
                     /locus_tag="PKV56_07655"
     CDS             complement(17997..19112)
                     /locus_tag="PKV56_07655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX67729.1"
                     /translation="MESNPAVSDRPHVVHLETGRHVYGGAQQVLYLTERLPALGVRCT
                     LVCAAGGEMATKARAAGTDVVELSTIGDADPSITWRFASLLRRLQPDLVHIHSRRGAD
                     VWGGLGARLAGVPAIMSRRNDNREAWLALKLKYPLYRHIVAVAQGIKDVLVEQGISGD
                     DITVVHSAIDPARFQHPRPRAELAATFGVDASQPILGIAAQLIDRKGHVLVFEALQRL
                     SSRHPTVQLIVFGRGPLSDSLPAEAAARGLQSRVCFAGFRQDLGQWIGALDLLVHPAY
                     TEGLANVCLQAGAAGVATIGTRVGGIPEAIADGRTGLLVPVGDAEALAAAIDRLLVDV
                     DLRHRLGAAGPAHVESDFTPSIMAAGNRAVYRRVIGR"
     gene            complement(19160..20146)
                     /locus_tag="PKV56_07660"
     CDS             complement(19160..20146)
                     /locus_tag="PKV56_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOX67730.1"
                     /translation="MTSITRQRAMQVLRLVGAPLLLAGVVWLAGPRAIWEAVQGADAG
                     WLLAGLLCATLGNASAALRWAELARWLGHRVHPRWALTVYFKAVAVNALLPGAVVGGD
                     MFRAWQLHRDGCARGAAGLSVVLDRLSGLWMLFVLSAMALFIGAGTPEMGMLRRMLHV
                     PEAWPSVLIALLLVTILLLLPLGGLIALAAVAGHRAEPGSRRAVAAGLLQRPAALEQY
                     LRQAAASLGVGIFAMAALYCAARGFGIELAAWLLVVTAAPIFLLAALPVSFGGWGTRE
                     AATVAGWAAFGVAPALSVGASAAFGIYALLQAALGLFPLPSAVRSDDGAAGV"
     gene            complement(20190..21203)
                     /locus_tag="PKV56_07665"
     CDS             complement(20190..21203)
                     /locus_tag="PKV56_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX67731.1"
                     /translation="MSTLLAEPVVLHVVAPEDAIDVSIVIPIYNEVESLPDLVARVHE
                     ALAPTGRRFELICTDDGSRDGSDRLLAQLAADRPWLKPVYLARNYGQSSALQAGFDSA
                     RGRYIVTLDGDLQNDPLDIPALLDRLDTDPAVDMISGWRQHRQDAAVSRKLPSMLANR
                     LISSSTGVHLHDYGCALKAYRREIIERIRLYGELHRFIPSLAREAGARIAEVPVRHHA
                     RSKGVSKYGIDRTFRVILDLVLIVFFMRYHQRPLHAFGGLGLWLATPGAAILGYLAVI
                     KAMGESIGGRPLLLAGVVLFLVGLQLIVAGLIGEMLVRIYHEGRGRPQFHTRGAPRMP
                     PAP"
     gene            21303..22808
                     /locus_tag="PKV56_07670"
     CDS             21303..22808
                     /locus_tag="PKV56_07670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOX67732.1"
                     /translation="MKSTAPHPDPKAPDGVTPAGRHGAVPPAAWLFGGLLVYFAAQLI
                     LRVLTAEGVELDEAEQLLWTQDLRLGYGPQPPLYTWVQWVFFQAFGASIFALALMKNL
                     LLLGTYTCTWLAARRLVAPPLAALSSASMLLLMQIVWESQRDLTHSVLVTTSAAATLA
                     LLVELLRRPRTGLYLLLGMTVACGLLAKYSFALFIAAFGGALLLTPGTRRVLLDRRLL
                     LSVAVVLLLVTPHALWVIDHLGEAVSSTAGKLSADRARGAGQIARGMLSLAGIWPSFL
                     SPLWVVLALFFGRGLSAQSEGRDPVVCGLFARYLLLLVALLAATVLIGGASHFNDRWL
                     QPLMFFAPLMFFVGWPGLLRHPRLHWLAWAAAAMALLTLTLLSLRPWYDGRRGHVGDL
                     NLPVQGIARAVRAAGIEPATIVSSNKHLAGSLRLAFPSARIIHSSANSPVLPAGPVLL
                     LGSAAGFDALRAQQPGWTDAPATTIEVPYAHARADAPKLKFTFAQAGSPAN"
     gene            complement(22817..23833)
                     /locus_tag="PKV56_07675"
     CDS             complement(22817..23833)
                     /locus_tag="PKV56_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOX67733.1"
                     /translation="MKVLVTGVAGFIGMFVAQRLLARGDRVVGIDNLNDYYDPTLKTD
                     RLARLSGAPDFVFERMDLADRDGMASLFRRERFDRVVHLGAQAGVRYSLTNPMAYVDS
                     NLVGFANVLEGCRHGGVEHLAYASSSSVYGGNAKLPYSEADSVDHPVSLYAATKRANE
                     AMAHSYSHLYGLPTTGLRFFTVYGPWGRPDMAPFLFADAMLSGRPIKVYNEGRMTRDF
                     TYIDDIVEGVLRVLDKPATADPLYDAAQPDPSTSSAPWRLFNIGNSTPVPLLEFIDTM
                     ERALGVTAVREMLPLQPGDVLATHADTSALDAWVGFSPATSLADGIQRFTDWFRPRYH
                     PQRR"
     gene            23907..25202
                     /locus_tag="PKV56_07680"
     CDS             23907..25202
                     /locus_tag="PKV56_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOX67734.1"
                     /translation="MPLSTPPSLAILSRLNRPLADLPTRCDVIVVGAGPAGSAAATTL
                     ARAGLDVVLVDQQAFPRDKVCGDGLIPDAHRALQRLGLLDAVLARAQSVKHVGVIGPR
                     GGRIDVPGTLAVLPRRELDLLLCSAAAEAGARMHAPVRFVGVIEEDEGQTTRVVGVRL
                     RSGDTTRELRAAWVLIASGAPPQALQAAGMCERRTPTGVAMRGYVKNPALVGRITELE
                     VVWHPRMKRGYGWIFPCPDGVFNIGVGVTHSHKAGHGPGQSMHDVNLRTMFDEFTRVH
                     PAARDLIAGGTWVGELKGAPLRCSLAGARLSRPGLLVTGEAAGSTYAFTGEGIGKALE
                     TGIAAAETLIEGRRSNWPEAQVRATYEDRVQALKPRYDMYDKAGIVNAHPWLVELVVW
                     SARRSPGRLRRMTGLLEETHLPGSLLTPRSWLRMLFVHS"
     gene            25214..25972
                     /locus_tag="PKV56_07685"
     CDS             25214..25972
                     /locus_tag="PKV56_07685"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOX67735.1"
                     /translation="MCQLLGMNGNTPTDIVFSFTGFSTRAEEHQDGFGIAFFEGAGLR
                     LFVDAQSARHSPVADLVRRYPIHSNNVIAHIRKATQGRIALENTHPFVRELWGRYWAF
                     AHNGNLTGYAPRLHASFHPVGDTDSERAFCWLMQELAKAHASLPPVAELTATLRELAP
                     QVAAHGSFNFMLSHGEALWAHCSTRLSYLVRQHPFQEARLQDEDISVDFARLTTPSDR
                     VAVIVTEPLTRDEAWVAFAPGELKVFVAGEPLCA"
BASE COUNT         4372 a         8828 c         8649 g         4317 t
ORIGIN      
        1 gcaggacatc gccgcgatcc gcgcgggggt gatgcggcac ttcaagaacg cctgtgtcac
       61 ggtggtgctg ggtcccgaag acaagaaccg ttcggacgcc gcgcccacca tcgccgcctg
      121 agcccgcatc agggcgccga gctggcaccg ccggccggtg ctggcaaaga tctgaagcgc
      181 gccgacccct gtcgaatccc ctgatggtcg gggacgcacg ttcccccaga atggtccgtc
      241 tttcccgaag tcggagcacc atggacaagc cttggctgaa gcattacccg ccgggcgtgc
      301 ctgcacggat cgacgtcgat gcatttccgt cgctggtggc gatgctcgat gacagtttca
      361 gcaggttccg cgaccgggcc gcctaccgct tcatgggcaa gtcgatcacg ttcgacgatg
      421 tcgaccaggc atcgaaggcg ttcgcggcct acctgcaggc gcagggcttc gcgaagggcg
      481 accgcatcgc gctgatgatg ccgaatgtgc cgcagtaccc ggtggccgtg gccggcgtgc
      541 tgcgcgccgg ctgcgtggtg gtcaacgtca acccgctcta cacaccacga gagctggaac
      601 accagttgaa ggattccggc gccacctgcg tggtgatcgt cgagaacttc gcgaccacgc
      661 tgcagaaggt gctgccgacg gtaccgatca ggcgggtcat cgtcacgtcg ctcggcgacc
      721 tgctgggagt cccgaagtcc tggatagtca actacgtcgt gcgccatgtg aagaagctgg
      781 tgcccccgtt cgagctgccc ggcgccgtgt ctttccgtgc cgcgctgtca gccggccgga
      841 acctgccgat gcagcctgta gccatcgggc ccgacgacat cgcggtgctg cagtacaccg
      901 gcggcacgac gggcatcagc aagggggccg tgctgctgca ccgcaacctg gtcgccaacg
      961 tcctgcagtc cgaagcctgg cacggcccgg cgctgaagaa gctcccgccg ggcgaacaac
     1021 tcgtgaccgt ctgcgcgttg ccgatctatc acatcttcgg cttcaacacg aacatgatgc
     1081 tggggctgcg catgggcggc tgcagcctgc tgatcgtcaa cccgcgtgac ctgccggcca
     1141 tgttcaacga actggccgcg gagcgcttcc acagcttccc cgcggtcagc acgctgttca
     1201 aggccatggc gagtcatccg gatttcggca aggtcgactg gagccggctc atcatctcgg
     1261 tcggtggagg catggcagtg caaagcgcca cggcgcagct gtggctgcag aaaaccggct
     1321 gtccgatcgt cgaaggttac ggcctgtcgg agacctcgcc cacggcgagc tgcaacccga
     1381 tcgacagcac cagcttcaac ggcaccatcg ggctgccggt gtccaacacc gagatgaagc
     1441 tgctcgatga tgcaggcgag gaggtcacca tgggcagccc tggcgaaatc gccattcgcg
     1501 gcccgcaggt gatggccggt tactggcagc gccccgacga aaccgccaag gtgatgactc
     1561 ccgacggttt cttccgatcg ggagacatcg gtacggtcga cgagcgtggc tacttcaaga
     1621 tcatcgatcg caagaaggac atgatcctcg tcaacggctt caatgtgtat ccgaacgagg
     1681 tcgaggaggt ggtctcgcag ctggctggca ttgccgagtg cgccgccgtg ggcctggccg
     1741 acgagaaagc cggtgaagtc gtgaggctgg tgatcgtgcg cagcgatccg caactcaccg
     1801 aaggtgcggt gaaggcccac tgcgaagccc tgctcgcagg ctacaagcgg ccccgggtca
     1861 tcgaattccg cgccgatctg cccaagacca acgtcggcaa gatcctgcgc cgagaattgc
     1921 gggatcgccg ttgatgtccg gctgcccgct ttgcgcctcc gcccatggct cctcgattct
     1981 tcgtcccgcc gccgctgcgc gcgggtgatg tccgcgccct gccgcccggc ccgacacgcc
     2041 acgtgcaggt gttgcgcctg cagcccggtg acgacatcac gctgttcgat ggcggcggcg
     2101 gcgagtggcg cgcccgcatc gatcgcatcg gcagaagcga cgtctcggcg cgcctgatcg
     2161 aacacgtcga gacgagcctg gaactgccat tcgacgtcac gctcgccctg ggcatgccgg
     2221 ccaacgaccg gatggacagc gtcgtcgaga aggctaccga gctcggcgtc gcaggcatcc
     2281 agccgctggt gtgcgagcgc tcggtgctga ggctgacggg cgagcgcgcc gacaagaagg
     2341 tggcgcattg gcaggctgtc gcagcttctg ccagcgagca gtgcggacgc acccgggtgc
     2401 ccacggtgca tccggtacgc acgctgccgc agtggctcgc cagcctgacg tcggacgcca
     2461 cggatcgtcg gctggtgctg agcctgcacg aaacccgccc gtggagtgtg gtcgctgctc
     2521 aggatcagcg cctgctgttc ctgagcggtc ccgagggcgg cttgtcggag gccgaggaac
     2581 aggcggcgct gcaggcggga ttcgagccga cgtcgctcgg gccgcgcgtg ctgcgtgccg
     2641 acaccgcgcc actcgccgtg ctagcggcac tggccctgca gttcacgcca aactgaaggc
     2701 gctcggccgg tcggttgtct tgtcatcgat cggccaccag aatcaggaga actcactcac
     2761 ggagattgcg atgggcttgc tcgattcggt catcggtgcg ttggcaggcg gcacccaagg
     2821 gaacacagcg ggagccggcg gtggccaggc ggcgttgatc agcatcgtgc tcggcatgct
     2881 gacgcagcgc ggcggcggcc tgggtgccga aggcgccgag ggcggcgtca gcggtatggg
     2941 tggtctcggt gatctgctgg cccggtttca gcaggctgga ctcggcgatg cggccgcttc
     3001 atgggtcggc accggcgcca accagccggt ttcgcccgat caggtcgagg gcgcactcgg
     3061 ctccgatctg ctgtcgcgga tcgcccgcca gatcggcatg ccccagggcg agacggccgg
     3121 ccagttgtcc gagttgctgc cgcaggtggt ggaccggatg acgccgaacg gccggttgcc
     3181 tgaaagcggt ttcaacgaag acgatatcgc cggattcgaa ggtctcgacg acctcctcgc
     3241 gcgcttctcg aaaccctgag ccttggcgag cccggccgga ttcagcgcag cgcagcgtcg
     3301 ccgcgcatgg tgccggccac cagatcgaag cggaacaggc ggcactcgat cgggccgttc
     3361 cacatcggcg tgcggcgcga ttccttcagt cgcatcgagc cgggcagctt catgtcggga
     3421 ctcaggacat atgccgtcca gccagcggga tgccgcgtgt aggcgcgctt ccagtgggcg
     3481 ctcaggcgag cgaagaagtc gtcgggcagt tccttgttgc cgggtccatc ggcgggcatc
     3541 gcaccacgcg ccgccttgcc ggcgacctcg atgcgttcgc cgtacggcgg gttcagcatc
     3601 agcgtgccag gcaggtcgtc gggcagatcg ggcgccgggc gctccagcgc atcaccgccg
     3661 ttgaaggtga tggccgcctc gacgccggca cgctgcgcat tgcggcgtgc gaaatcgacc
     3721 atgcggaaag acacatcgct ggcgtggatc ggcaccgccg acgcatggat gcgcgactgc
     3781 gcgtggctgc gcagccgctg caactccgcg cgcatctcgg gcgtcgcgaa cggcagcaag
     3841 cgctcgaacg cgaagcggcg cttcagcccc ggcgcgatgc cgcaggcgat ctgcgcggcc
     3901 tcgatcgcga tggtccccga accgcagcag gggtcgtgca gcgcgccgcc cgcgtcgggc
     3961 cggccctgcc atccggcagc agcgagcatc gccgcggcca gcgtttcctt gagcggcgca
     4021 tctcccttgt cctcgcgcca gccgcgcttg aacagcggct cgcccgacgt gtcgacatac
     4081 agcgcggcgc gctcctcgcc gaggtgcagc accagagcca ggtcggggtt gcggacatcg
     4141 acgctaggcc gctcgccggt gccatcgcgc agccggtcgc acacggcatc cttgacgcgc
     4201 agcgcagcga aattgaggct gcgcaacggg cttcgatgcg ctgtggtgtc gacgcgcagc
     4261 gtgtgctcgg gcgtgatcca ctgcatccag tcgacgctgg cagccaggtc gtacaaggcc
     4321 tgctcgtcgc ggtacggacc ttcggcgacc tcgaccagca ctcgctgtgc tagtcgggat
     4381 tcgaggttga gcaccatcac ttcatgcggg ccaccgtcga gcgcgactcc gccacgcaac
     4441 gtgtgcactg ccgtgtcagg cagcacgcgc tgcacctcgg tggccaggag ggcctcgacg
     4501 ccagcggcgc aaggcaggaa aaaaggagcg gacatgcgcg gaattacatc gtcttgcgca
     4561 ggttggccgg agcaatcctc aacgcttcgc gatatttcgc caccgttcgg cgcgcgacct
     4621 ggatgccctg ttcctcgagc atcttgctga gctggctgtc ggacaacggc ttcagcacgt
     4681 tctcggccgc caccagctgc ttgatcaatg cacgcaccgc ggtactcgac gcgttgccac
     4741 ctgcgtcggt gttcagcgaa gagccgaaga aatacttcag ctcgaacgtg ccgaacaccg
     4801 tggccatgta cttggcggtg gtcacgcgcg agatggtcga ctcgtgcaaa cccagctcgt
     4861 cggcgatctc gcgcaggacc agcggcttca tcgcgatctc gccatgggtg aagaagttct
     4921 tctggcgctc gacgatggcc tgcgacacgc gctggatggt gtcgaagcgc tgctggatgt
     4981 tcttcatgaa ccagcgcgcc tcctgcaggc gcgaactgag cccggcgtga ctgccgttgg
     5041 cattcgccgc accccgcccc tgacggatcg cctgggcata caggtcgttg atgcgcagct
     5101 tgggcatcac gtccgggttc agcacgacct tccagttgcg gcccgacttc tgcacgatca
     5161 cgtccggcac gatgatgttg gcctccgcgc gcgcaaaagg ccgtccgggc ttgggctcga
     5221 gcgcggtgat gagcgactgc gcctccttga ccaggtcctc gtcggcgcct gtcacggcca
     5281 tcagcttctt caggtctcgg cgagccagca gttcgagatg ctgcttgcag atcatgatcg
     5341 cgatcatctg agcctcgctg cgtggcagca gccgcaactg cagggtcagg cattcgggca
     5401 ggtcgcgcgc gccgacaccg atcggttcca tgttctgcag ccatttcagc gcacaactca
     5461 ggcgctccat cacctcctcg aggcggtctt catcgtcgcc agccagcgat gcagcgatct
     5521 cctccaggct gtcagccagg tagccgtcgc cgtccagcga ttcgatcagc acctcgaccg
     5581 ccgccgcatc ctcgggcgac aggcgcattc cgagcaactg agtacgcaga tggtcctgca
     5641 ggctggccgg ggtcgggttg cggtcctgtg gatcgaagtc ctctccgtcg ctgcctgtcc
     5701 cagccttgcc gggcgtctcg cggatgccat cgaagtcttc acgctcggtg ccgttctccc
     5761 agtcgtcgcg ctcggtggtg ccgaactcgg ccgcatcgac tcccggggcg tcgtcaccgc
     5821 cctcattgct cgactcagac ggtgtgtcgg cacgttcggt ctcgcgctcg gccacccgtt
     5881 cggtcggcgt cgaacgctcg accatcggct cgaatgcggg caggccttcg tcctcggttt
     5941 cgaggaacgg gttctgatcg agcatctgct cgacttcctg atgcagctcc agcgtggaca
     6001 actgcagcag tcggatcgac tgctgcaact gcggcgtcag cgccagatgc tgcgacagcc
     6061 gtacctgtaa cgacggcttc attgggcagc gagcggagcg aagcctccgc catcaaacca
     6121 cgcccgtggc acgggtttcc cgggccacca ggcggcgccc cctgggggca ggagtgaaac
     6181 gactgggggg cccacgttca catccggaag ttttcgccga gatagacctt gcgcacgtcg
     6241 gggttgtcga cgatctcgga cgaggtgcct tcggccagca cccgcccttc gctgatgatg
     6301 taggcgcgat cgcagatacc cagcgtttcg cgcacgttgt ggtcggtgat cagcacaccg
     6361 atgccacggg tcttcaggaa accgatgatt cgctggatct cgatcaccgc gatcgggtca
     6421 acgccggcga atggttcgtc gagcaggatg aagcgcggct gagtggccag ggcgcgcgcg
     6481 atttcgacgc gccgacgctc acctccggac agcgccagag ccggcgaatc gcgcagcttc
     6541 tcgatgccca ggtcgcgcag caatccttcg agcaattcgt cgatcaccgc cggtctcaac
     6601 gtcttgccgt tggcgtcgag ttgaagttcg agcacggcac ggatgttttc ctcgacattc
     6661 agcttgcgaa agatcgaggc ttcctgcggc aggtaggaca ggccgaggcg tgaccgcttg
     6721 tggatgggca gacgctccac gcgttgcccc tcgatggtga tctcgccggc atcggcaggc
     6781 accagaccca cgatcatgta gaaactggtc gtcttgccgg ctccgttggg cccgagcagt
     6841 ccgatgactt cgcccgcatc caccgaaagg tgcacgtcct tgacgacctt gcgtgcgcca
     6901 tagctcttct gcagcccggt cgcactcagg cggtgaaccg caggccgagc ctctgccgcg
     6961 gctgtgttca acgcttgctg cccagcgccg tgctgggctt cagggtgacg cccccggctg
     7021 acgacgccgc ttcggcagcg ggcgtgactt cacgcggcgt gagcacggcg cgtacccggc
     7081 cactcgggtt ggtgggcgtg gcagccgcgg ccccacccac cacgctgaac acttcggccg
     7141 tgttgtcgta gacgatcacg ctgcctgcgg tttcatcggc cagcgtcgaa ccgcggaaac
     7201 gccggatcac cgcacgattg acgaagcgga tggtgtcggc cttgccgtca tattcgatcc
     7261 gctcgccttc gccttcgatg tactcgtcga gtccttcccg cttctgccgg aagacggccc
     7321 gcttgtccgc atctgccgtg gcaacgccgt actgcgcgcc atcgggtgtc tgacgcacct
     7381 cgatcttggc agcgcgcatc gacatggtgc ccttggtcac caccacgttt ccagtgaagg
     7441 tgcccatctg cttggcgtcg tcgtatcgac cgttgtccgc ttcgatgttc agcggcttgg
     7501 acttgtcagc cttctcggca tgaacgaacg gcgcgccgat cgcggcaacc gcgaccacga
     7561 cgatcaaccg caagcgagtc gaccagggaa gaggtgaagc ggatgtgagt gggttcatgg
     7621 ggcacgattc ttgcaggaaa ttctatccca caagatcgtg tcgcgccgac tggccatcat
     7681 ctatttcgtg cgctttcacc aacactcgca gcgaaagacc ttgcctgcca gtcgaccctt
     7741 caggtgttct tgcgaacccg gttcagcagg tagtctgcca cggccaggga ccgctcaccg
     7801 gatcctgcca tcgaacccga ggtgtagtag cgcccctgga tgccgatcgc aggaacgccg
     7861 tcgatacggt acgcctctgc cagctgtttc gcctgacggg ccttggtctg caccgagaag
     7921 gagttgtaga cctccgagaa tttagcgccg tcgactccgt tcttgttcat gaactcgacg
     7981 agttccggca gtttgtccaa gcgggcgcga tccaggtgca ccgcgttgaa caccttgcgg
     8041 tgcaacgtgt ccaccaggcc catcgtctcg atcgcgaaga agagccgctg gtgcgtcgtg
     8101 aagggctcct cgcgaaacgc cacgggaatg cggcgaaacg ccacgtcggc cggcagcttc
     8161 ttctgccatg cctcgagagc gggttcgaac gcgttgcagt gcggacagcc gtaccagaag
     8221 aactccacca cttcgatctt gccgccacct ggaaccgcct gcggctggct cagcttcacg
     8281 tagtgctgcc cttcgacgaa atcgccctga gcgtgcgcgc tgaagacagc ggatcccacc
     8341 gtggcaccgg ccaccgtggc cagcagttgc gttgaaaact cgcgtcgctt catgattctc
     8401 gattccttcg tcgtgaccca ctgcagcggt cggtacccgc cccgcaaata tgccccgcaa
     8461 ccccaactga gggcggccgc cgccaaaagt tcaggcccgt caaaaccatt tcacccatcg
     8521 tgtcgcgcat cagcgctcat tttggggcgc gtaccagcac ggcttcgaac ccaccggcaa
     8581 gcagcttctc cttggtgccg tccgcctcga ccttctcctc gaacgggccc agacgcaccc
     8641 ggtgcacggt gcgtcctgac tgttcgcgct cggtgacccg cgccgaataa cccagcagcg
     8701 ccagcttgcc ccgctgcgat tcggcatcct ctgcgctggc ataggcaccc gtctgtacga
     8761 agtagctgaa cgcttcgtcg gccgatccgg tggacacggg cgtcgaagcc ttgcctgcag
     8821 gctccggacc cgatgtcgca cgatcggcca ggatgctggc cggatcgcgg ccgcttcctg
     8881 ccgggggcgc tccactggcc ggggcagaag ctgcggcgac agaggatgcc ggcctcgccg
     8941 ggttcttccc ggccagaggg gcattcggat cccagttctt gttcttctcg cgctcggctg
     9001 cgtcctgctc ttccgtgcgc tggggcacct tgtcgaggaa gggcaccggg accttggtca
     9061 cgtacagggc gacacccagc gacagcgcca gtccgatcag caagccgacg atgacaccga
     9121 cgaagaaccc gccccgctga agggacaggc tgtgacgggg gcgtgcgcga gttcgcatgg
     9181 cagggctcac attttctcgg gcgcgctgac tcccagcacg gtcagggcat tgcggatcac
     9241 ctgacgcgtg gcactgagca gcgcgaggcg cgcctgggtc aatgccgcat cgtcatcgac
     9301 gaggaatcgc tccgcgccgt aatagctgtg aaacgcgctg gcgacatcgc ggaggtagaa
     9361 ggtcacgtcg tgcggcgcca gactctccgc agcgctggtc agcatggccg gatagtcggc
     9421 cagcttgagc atcagtgcgg tttcggtcgg cgcggtcagc acggacacat cggcgcgccc
     9481 gatggcgtcc ggatcgccgc ccttgtcgac gtattgctgg atcacggagc agatgcgcgc
     9541 gtgcgcatac tgcacgtaga agacggggtt ctcgtcgttc tgcttgagcg ccagatcgac
     9601 gtcgaaggtg aattcggtgt cggccttgcg gctgatcagg aagaagcgca cggcgtcgcg
     9661 actggtccag tcgatcagat cgcgcagcgt cacgtacgag ccggctcgct tggagatctt
     9721 gacctcctcg ccgcccttca tcaccgtgac catcttgtgc agcacgtagt cggggtagcc
     9781 gggcgcgatg ccgacccccg ctgcctgcag ccccgcgcgc acgcgggcga tggtgccgtg
     9841 gtggtcgccg ccctggatgt tgatggcctt ggtgtagcca cgctggaact tgctgatgtg
     9901 gtacgccacg tccggcacga agtaggtgta ctgtgcttcg ccatccgccg aggcgccggg
     9961 ggccggcgac ttgcgcatca cgcggtcctt gtcatcgccg tagtcggtgc tgcgcagcca
    10021 gagcgcgccg tcctgttcgt aggtcttgcc ggcggcgacc agacgcgcca cggtggcctc
    10081 gacctggccg ttcgcgtaaa ggctcgactc caggaaatag tggtcgaagc gcacaccgaa
    10141 ggcctgcaga tccagatcct gctcgtggcg caggtaagcc acggcgaact ggcggatgcc
    10201 gtcgagatcg ttcacgtcgc ctgacgcggt gaactcgcga tcgtcggcct tgactgtctt
    10261 gcgggccgtg aaatcggcgg cgatgtcagc gatgtagtcg ccgttgtagg ccgattcggg
    10321 ccagtgcgca tcgccaggct ggtagccacg caggcgtgcc tgggtcgatg ctgccagcgt
    10381 cgcgatctgc acgccggcgt cgttgtaata gaactcgcgc cggacatccc aaccctgggt
    10441 ttcgaacagg ttgcagatcg catcaccgag cgcagcctgc cggccgtggc cgacgtgcag
    10501 cggccccgtc gggttggccg acacgaactc gaccagcacg tgctgcgcat tggcggcctg
    10561 gcgcccgaaa cacgcgccgg cagccagcac ctcgcggacc acggcctgcc tggccgaggc
    10621 gcgcagccgc aggttgatga acccgggccc ggcgatctcg aaggattcga catggcgctg
    10681 tacagccggc tgacgttcga gcgcggcgat cagcgcctgc gccacctcgc ggggattctt
    10741 cttcagcggt ttggccagct gcatcgccga ggtgcatgcc aggtcaccgt gcgcggccag
    10801 cttcggcgac tcgaaagcgg ccttcagggg actcgccgga ctgacttcct cgatcgctga
    10861 ggcgagtgcc ttcaacaggt cttgtttcgc ttcaatcatc gaacgattct acgtttcggt
    10921 cctttttcga tcggcgcgct gcctgcgcat cggtttgagg ggtgtttgga cgcttgtttg
    10981 ccgcaatcga ccggctgtca ctggtagacc atcggaggac gcggcgctgc aggcgcggcg
    11041 ttgccgcctt tcacttctgc cgacgcccat ggcccctacc gaaaccgcca gccgaccgac
    11101 ggacagcacg gtcgacatca cgaccgattc cgcaccgact cggccgcggg tggtgctcga
    11161 cacgagcaag tcgatgcccg agcagatcgg caagtacccg gtgctgcgca agctcggaga
    11221 aggcgccacc agcgaggtct acctgtgtcg cgacgagttc caggccaggg atgtcgctat
    11281 caagcgcgtc cgcgccgctt ccacgtccga cgcagtggcc aatcgctaca gcgagcggtt
    11341 cttcgccgcc gaggccgcac tggtcggcag gctccagcat cccaacgtcg tcaagatcta
    11401 tgacgcggtg tccgacgcat cgagccagta cctggtgatg gagtacgtgg ctggcacgac
    11461 gctgcggccg tactgccgag ccgaccagct gcttccgctc gagctgatcg ttgaaatcgg
    11521 cttcaagtgc gcgatggcac tcggctacgt ctaccggcag ggattgatcc accgcgacgt
    11581 gaagccggcc aacgtgttga ccgtgctgac caacggagcg atcaccgacg tgaagatatc
    11641 ggacttcggc agtgtgctga atctcgattc ggacgtgacc caggtctacc gtgtggggtc
    11701 gctggcctac atgtcccccg agcagctcga cggcagctcg ctggactgca gagcggacat
    11761 gtattcgctg ggcgcggtgc tttaccacct gatcgccggg cgaccaccgt tcgacgcgca
    11821 gatccagtcg gccatgatgc atcagatcta ccacgcgaca ccggcctctc tggtcggcat
    11881 gcgcgatggc gtgggtgtcg ccgtggacag cgtgatccag cgcgcgctcg ccaagcaccc
    11941 gcaggaccgc tatcccgact gggatgcgtt cgcccaggcg ttgtcgggcc tgatttccaa
    12001 tcaggaggta ccgcgcgggc agttgcaggg cgtgctcgac tccgaacgct tcaacatgtt
    12061 gcgcaagctg gaattcttcg cgagtttcgg cgacgtcgag ctgtgggagg tggtgcaccg
    12121 cgcgaagtgg caacgctttc cggtgggtca cgcgctctac cgcaagggtg aagaaggacg
    12181 caccttccac atcattgcgc agggagagct cgaggtgttc cgcgaaggca acaaggtcgc
    12241 ggtgctggga gcgggcacgt cggtcggaga gatggcctat ctggcgccga gccccgatct
    12301 gcgccgccac agcaccgacg tgctgatcag cgagcaggcg acgaccatct ccttcgttcc
    12361 cgaaacgctg gcgcaggtca gcccgaactg ccggcacctg ttcgacgacg cgttcatccg
    12421 cgtgctggtg cgccgactgc acgcagcgca tgaggccctg gcgcaccctc gacgcatcct
    12481 ttgatcctcg ggagttctcg ccggcggcgg gcgccgctgt cgtgtgcacc gccagcccgg
    12541 ctggcgcgtt gtgtcggctg ctgccgcggc cacgcatcgt tcagaagcgg ttcagcgttg
    12601 tttcacaact ccaccacccc acgaggacgt tccatgaaaa acctgatgat cgcgctgatc
    12661 gtgtgcaccg ccgccctgtc cggcacggcc tacgccaaca gccacaagga agctcccgag
    12721 atggcggccg cagccagcgc gcccgagatg ggcaagcagc aaagcaagat gaccacctgc
    12781 aacaaggatg cgggcgagat gaagggcgac gagcgcaagg cgttcatgaa gaagtgcctt
    12841 tccgccaaga agccgacgca gcaggaaaag atgaagacct gcaacgccga agccaagggc
    12901 aagaccggcg aggagcgcaa ggacttcatg aagagctgcc tgagttcctg agccgcgcgt
    12961 cggggcagcc gtcacccctg ggggctggct gccctccagt aggtcgcatc gccgtaggtg
    13021 tgcttcagga aatcgatcca cagacgaacc cgcagcggca ggtgcttgcg ctgagggaac
    13081 accgcataga tgccattggg cggggatgcg aactcgtcga gcacgctgac cagccggccg
    13141 ctcgccacgt cctgctcgac ctcccacatc gagcgccagg cgatgcccag accggccaga
    13201 caccactcgt gcagcacctg accatcgctg caatcgaccc tgccactcgg tctcaggtgg
    13261 gttgtcaccc cctccagcgt gaacgcccag ccgcggggct ggctggcgtc gctgctgaat
    13321 gtcaggcatt cgtgctgata cagatcgctg ggctgcgtcg ggatgcccgc gcgctgcagg
    13381 taggccggtg aagccacgca cagccggcgg ttgtcggcca gccgcacgct gaccaggctg
    13441 ctgtcgggca ggtcacccac ccgcaccgcg cagtcgacgc cttcgttgac gatgtcgacg
    13501 atgcggtcgc tgaggctcag ggagacgttc acatccggat gcagtccgat gaagcgcggc
    13561 accagcgtcg ccacatgccg acgcccgaac cccgccggcg ccgtcacccg gatgtacccg
    13621 ctggccttga cgccaccggc actgacgctc gcttcggcat tgttgaggtc ggccagcagg
    13681 cgctggcagt cttcgaggaa agcgcttcct tcgtgcgtca gcgtgatccg tcgcgtggtg
    13741 cgcaccatca gtttcacgcc cagccggctt tccagcgcat cgatgcgccg cccgatgacg
    13801 gccggcgcga ccccttcgac cagcgcggcc gcggtcaggc tgcccttggt tgccacctgc
    13861 gtgaacgact cgatctgctt cagcttgtcc accgtcagct cctccagtcc cgccagatta
    13921 ccaaccaaaa agtcatgaat gcattgtctt atcagggatt aatcgcggaa taggtctgga
    13981 ataaagtttc cgaatgtccg ctccagcact ccgcccgcgc ccgctagccg tgctgttcga
    14041 tgcctacggc acgctgttcg acgtctacag cgtcgccacg ttggcagagc agctctttcc
    14101 cgggcaaggt gagcgtctgg ccacgctgtg gcgcgacaag cagatcgagt acacccggct
    14161 gacctcgatg agcagctcca ggggcgagca ttaccggccg ttctgggagc tgacgcgggc
    14221 cggcctgaag tacgccacgg cgcgcctggg gctggcgctc gacaccgcga ccgaggagcg
    14281 gctgatgaac gaataccggc acctcggcgc gtttcccgag aaccatgagg tgctgaccgc
    14341 gctgaagtcg cgcggcatcc cggccggcgt cctgagcaac ggcgaccccg acatgctggg
    14401 cgtcgccgtc aagagcgcgg gcttcgcggc gttgctgtcg ccggtgctca gcgcgcatcc
    14461 gctgcgcaag tacaagaccg acccggccgt ctacgcgctg ggtacgcagg cgttcgagtt
    14521 gccgggcaag gacatcctgt ttgtctccag caacggctgg gacgcgatgg gtgccagttg
    14581 gtacggctac accacgctgt gggtgaaccg cgcaggaatg ccgcccgaaa ccctgggccc
    14641 cgaaccgacg cgcaccggca ccagcctgcg cgacgtcctg agattctttg aaccctgatg
    14701 gagaacacca tgagtcaaac ctcgtcatct gcggcaggca cgctgtcgct gcccgccggc
    14761 atgcagatca acgcaccgat cctgcctgga ttcgagtcga tactgaccct gcccgcgctc
    14821 gaactggttg ccaagctgca ccgcagcttc gagccgactc gccagaagct gctggccgct
    14881 cgcgtggtcc gggcgcagcg tctggatgcc ggcgagcgcc ccgatttcct cgctgaaacg
    14941 aagcacatcc gtgagggcga ctggaagatc gctacgctgc cgaccgcact gcaccgccgt
    15001 cgtgtggaaa tcactggtcc ggtcgacgcc aagatggtca tcaacgcctt caactcgggc
    15061 gccgacagct acatgaccga tttcgaggac agcaactcgc cgctgtggac gaaccagatc
    15121 cagggccaga tcaacttggg ccaggcaatc cgccgcacgc tgacgctcga cgcgaacggc
    15181 aagcactaca agctgaacga ccagatcgcg accctgcagg ttcgtccgcg cggctggcac
    15241 cttgacgaga agcacgtgct ggtcgatggc cagcgtgtcc atggcggcat cttcgatttc
    15301 gcgctgttca tgttccacaa cgcgaaggag ctgctggcac gcggcgccgg tccgtacttc
    15361 tatctgccga agatggaaag tcacctcgag gcgcgcctgt ggaacgacgt gttcgtgatg
    15421 acgcagaagg aactgggcct gccgcaggga acgatcaagg ccaccgtgct gatcgagacc
    15481 atcctcgccg ccttcgagat ggaagagatc ctctacgaac tgcgcgagca cagcgccggc
    15541 ctgaacgccg ggcgctggga ctacatcttc agctgcatca agaagttcaa gctcgaccgc
    15601 gatttctgcc tggccgaccg cgccaaggtc acgatgaccg cgccgttcat gcgcgcctat
    15661 gcgctgctgc tgctgaagac ctgccacaag cgcggggccc cggcgatcgg cggcatgagc
    15721 gcgctgattc cgatcaagaa cgatccggcg aagaacgaag tcgcgatggc cggcatcatc
    15781 gccgacaagc gccgcgatgc caccgatggc tacgacggcg gatgggtcgc gcacccgggc
    15841 ctggtcgagg cgtcgatgaa ggaattcgtc gcggtgctcg gcgaccggcc gaaccagttc
    15901 gacaagcaga agcccgaggt ccaggtggct gcggccgacc tgctgaactt ccagcccgaa
    15961 gcgccgatca ccgaggccgg gctgcggatg aacatcaacg tcggcatcca ctacctgggt
    16021 gcctggctgg ccggcaacgg ctgcgtgccg atccacaacc tgatggaaga cgcggccacg
    16081 gcggagatca gccgcagcca ggtctggcaa tggatccgca cgcccaaggg cgtgctcgac
    16141 gacggtcgca aggtcaccgc ggacctggtc cgttcgctgg tgccggagga actggcgaag
    16201 atcaaggccg gtggcttcga gggcaagttc gaccgcgccg ccgggatctt cgaggaaatg
    16261 agcacgcagg acgcgttcgc cgagttcctg acgctgccgc tttacgaaga actctgagtt
    16321 cgccatgcac tgacaggcgc cggtgatccg gcgcgttcct tccaccctcc caccctcccg
    16381 agcgcttgca ccatgcccca tctcctgcac atcgagtctt ctccgcgcaa gcagcgctcc
    16441 gcctccatcg aggtggcgcg tgccttcatc gacacctggt gcgcacgcca cccgcaggga
    16501 agcgtggaca cgctggacgt gtgggcgacc gcgctgccag agttcgacag tgcggcactg
    16561 gacgcgaaat acgccggtct cgaaggccgc acccgcagcg acgacgaggc ccgcgcgtgg
    16621 tcgggcatcg aggcgctggg ccagcgcttt catcgtgccg acgtgatcgt gttcagcgtg
    16681 ccgatgtgga acttcggcat cccgtaccgg ctgaagcacc tgatcgacgc cgcgacccag
    16741 aaggacacgc tgttcacctt cgacgaacgc ggtctgctcg gcttgctggg cggcagaagc
    16801 atggtgctgg tggccgcgcg cggcgtggcg ctgggcgatg actttccggc ggcgatctac
    16861 gaccaccagg tgtcctacat gtcgatgtgg gctcgcatgg tcggcatcac cgaggtccac
    16921 cacgtgaccg tggaaaagac gctgttcggc cccgaggccg acaccgcctc gcgggaacag
    16981 gccaaagcgc aggcgcaggc cctggcgcag tctctctgac ggtcaaccgg aagcgacacg
    17041 cgtcgcccga agaccgtcag gcgagttccc gacgcatcag gcgctccagc acacgcagcg
    17101 gcgacttggc cgggtcgacc atgccggcga ccggcaggta gaacgtctgc tcgaagaggt
    17161 actgaccggc catcgcggcg tgcgaggcct ggtcggtaaa gaccagccag gtctggcccg
    17221 gggggaactc gtgcgtgcgc tgctcgacct cggcctggta cttggtatcg gccttcatcc
    17281 ggtcgtgcag ttgcagcatg aaatggtcat aggccgttcg gcgccccttg gtgatgcgca
    17341 gcagctgcag cagttcgcag ctgcccggca acggacggtg cagcgaaggc aggtagcgcc
    17401 gcgcaacccc ttcgaacgat tcgcccaacc gccaggtgcg cgtgcggcac tgcggattca
    17461 cgttggtgaa caggcgcagg atgcgccggc caccggtggg cgacgatgga aagctgtcga
    17521 catgcaggcg cgtgtcgtcc ttgcgccacg aggtctcgcg cccggccact tccaccggcc
    17581 ggaagctggt gcgcccctgc tgcaccgtcg aggcataagg ggagagcagc tgccgcatca
    17641 gcgtcaggct cgatgacgcg taacgctcca tcatgccgtg cagcgcagcg agttcggctc
    17701 ctgccgcact gcttccacgc acgctgccgt cggacggatt caggctgacg ttcttggctg
    17761 caccggagat gtctggcgac aggaactggc gctcgacatc gagcagcgga aagtcgagct
    17821 gcggcagcag caggacgttc ccgtcctcga gggaccggat cgcggcaagt tgttcatcct
    17881 gcgaactggg ctggtcccag ctcgcgatgg gcagactgat gatgtcggtc atggcgtggg
    17941 tgcgggttgg cgtccggtct tatagcgcga tgagcacgac ccggggtcgg cgcggttcat
    18001 cgaccgataa cgcgccggta cacggcgcgg ttgcccgcgg ccatgatcga cggcgtgaaa
    18061 tccgactcga cgtgggcggg acccgccgcg ccgagccgat gccggagatc gacgtcgacc
    18121 agcaggcgat cgatcgctgc agccagcgct tcggcatccc cgacgggcac cagcaggccc
    18181 gtgcggccgt ctgcgatggc ttccgggata ccgccgacac gcgtgccgat cgtcgccacg
    18241 ccagccgcac cggcctgcag gcacacattg gccaggcctt cggtgtaggc cggatgaacc
    18301 agcagatcga gcgcgccgat ccactggccg aggtcctgcc ggaacccggc aaagcacacg
    18361 cgcgactgca aacctcgcgc tgccgcttcc gccggaaggc tgtcggacag cgggccgcga
    18421 ccgaacacga tcagctgcac cgtgggatgc ctgctcgaca accgctgcag cgcctcgaaa
    18481 accagcacgt gacccttgcg gtcgatcagc tgggcggcaa tccccaggat gggctgcgac
    18541 gcatcgacgc cgaaagtcgc ggccagctcg gcccgcgggc gcggatgctg gaaccgcgca
    18601 ggatcgatcg cgctgtgcac gacggtgatg tcgtctccgg agatgccctg ctcgaccagc
    18661 acgtccttga tgccctgcgc caccgcgacg atgtggcggt aaagcgggta cttgagtttc
    18721 agcgcgagcc aggcttcgcg gttgtcgttg cggcgggaca tgatcgccgg cacgccggcc
    18781 agccgggcac ccagcccacc ccagacatcg gcaccacgac ggctgtggat gtgcacgagg
    18841 tcgggctgca ggcgccgcag cagcgaggcg aagcgccagg tgatcgacgg gtcggcatcg
    18901 ccgatcgtcg acagctcgac aacatcggtg ccggcggctc gcgccttcgt cgccatttcg
    18961 ccgccagcag cacacaccag cgtgcagcgc acacccagcg cgggcaggcg ctcggtcagg
    19021 tagagcacct gctgggcgcc accgtacacg tggcgtccgg tctcgagatg cacgacatgc
    19081 gggcggtcgg aaactgccgg gttcgattcc atcagggatg ttctcaaggg cttgctatcg
    19141 aatgaacggg ccgagacatt cagacacccg cggctccgtc atccgaccgc accgccgaag
    19201 gcaggggaaa cagtccgagc gccgcctgca acagggcgta gatgccgaag gcggctgacg
    19261 caccgaccga cagcgccggc gcgacaccga aggcagccca gccggccacc gtggcggcct
    19321 cgcgcgtacc ccagccacca aagctgacag gcagcgcggc cagcaggaag atcggtgcgg
    19381 ccgtcaccac gagcagccag gccgccagct cgatgccgaa gccgcgcgcc gcacagtaga
    19441 gcgcggccat cgcgaaaatt ccgacgccca gcgacgctgc cgcttgccgc aggtactgtt
    19501 ccagcgcggc cggcctttgc aggagtccgg cggccaccgc ccgccgactg ccaggctccg
    19561 cacggtgacc agcaacggcg gccaacgcga tcagtccgcc cagcggcagc agcagcagga
    19621 tggtcaccag cagcagcgcg atcagcaccg acggccacgc ctcgggcaca tgcagcatgc
    19681 ggcgcagcat gcccatttca ggtgtgcccg ccccgatgaa gagcgccatc gcgctcagga
    19741 caaacagcat ccacaggccg ctcagccgat cgagcaccac ggacagcccg gccgcaccac
    19801 gagcgcagcc gtcgcgatgc agctgccagg cgcggaacat gtcaccgccg accactgcgc
    19861 ccggcagcag cgcattgacg gcgaccgcct tgaagtagac cgtcagtgcc cagcgcggat
    19921 gcacccggtg tccgagccag cgcgccagct cggcccagcg cagggccgcc gacgcattgc
    19981 ccagcgtcgc gcacagcagg cccgcaagca gccagcctgc gtcggcgccc tgaaccgcct
    20041 cccagatggc ccgcggcccg gccagccaca ccacgcctgc gagcaacagc ggcgcgccga
    20101 ccaggcgcaa cacctgcatc gcacgctggc gcgtgatcga cgtcaacggc aggccccacc
    20161 gctcgcatcc tgcacccggc agcccacgct catggcgcgg gcggcatccg tggtgcgccg
    20221 cgcgtgtgga actgcggccg tccgcggccc tcgtgataga tgcgcaccag catctcgccg
    20281 atcaggccgg ccacgatcag ctgcagtcca accaggaaca gcaccacgcc ggccagcagc
    20341 agcgggcggc cgccgatcga ttcgcccatc gccttgatca ccgcaaggta gccgaggatg
    20401 gccgcgccgg gggtggccaa ccacagcccg aggccgccga acgcatgcag cggacgctgg
    20461 tggtagcgca tgaagaagac gatcagcacc aggtcgagga tcacgcggaa cgtgcggtcg
    20521 atgccgtatt tcgaaacgcc cttgctccgc gcgtggtgac gcaccggcac ctcggcgatg
    20581 cgcgcgccgg cctcgcgtgc cagcgacggg atgaaacgat gcagctcgcc gtacagacgg
    20641 atgcgctcga tgatctcgcg gcgatacgcc ttcagcgcgc agccgtagtc gtgcaggtgc
    20701 acgccggtgg atgaggagat cagccggttg gccagcatcg acggcaactt gcgcgagacg
    20761 gctgcatcct gccggtgctg ccgccagccg gagatcatgt cgactgcggg gtcggtgtcg
    20821 aggcgatcga gcagcgccgg gatgtcgagc gggtcgttct gcaggtcgcc atcgagcgtg
    20881 acgatgtagc gtccgcgcgc gctgtcgaag ccggcctgaa gcgcgctcga ctgtccgtag
    20941 ttgcgcgcca ggtagaccgg tttcaaccac ggccgatcgg cggcgagctg cgccagcaga
    21001 cgatcggagc cgtcacgcga accgtcatcg gtgcagatca actcgaatcg gcggccggtc
    21061 ggtgccagcg cctcatgcac gcgagccacc aggtccggaa ggctctcgac ctcgttgtag
    21121 atcgggatca cgatcgacac gtcgatggcg tcttccggcg caacgacatg caggacgacc
    21181 ggttcggcga ggagtgtgga catgggtggg gacgggggca aaacggcaat cggcaatgcg
    21241 gcgctcgcca ccgcggattc tagggagccc gtccggcagg cctgtgtatc cttcgccgcc
    21301 acatgaagtc gactgcgccc catcccgatc caaaggctcc ggacggggtc actccggccg
    21361 gtcgccacgg cgctgttccg ccggccgcct ggctgttcgg cggcctgctg gtgtacttcg
    21421 cggcgcaact gatattgcgt gtgctgaccg ccgaaggggt cgagctcgac gaagccgaac
    21481 agctgctgtg gacgcaggac ctgcggctcg gctacggccc gcagccgccg ctctacacct
    21541 gggtgcaatg ggtgttcttc caggccttcg gagcatcgat cttcgcgctg gcgctgatga
    21601 agaacctgct gctgctgggc acctacacct gcacctggct cgccgcacgc cgactggtcg
    21661 ccccgcccct cgcggcgctg tcgtcggcat cgatgctgct gctgatgcag atcgtctggg
    21721 agtcccaacg cgacctgacg cactcggtgc tggtgaccac ctcggccgcc gcgacgctgg
    21781 cgctgctcgt cgaactgctg cggagacccc gaaccggcct gtacctgctg ctcggcatga
    21841 cggtggcctg cgggctgctg gcgaagtaca gcttcgcact gttcatcgcg gccttcggcg
    21901 gtgcgctgct gctgacgccg gggacccgac gcgtgctgct ggaccgccgc ctgctgctga
    21961 gcgtggccgt cgtgctgctg ctggtcacgc cgcacgccct gtgggtgatc gaccacctcg
    22021 gcgaagcggt cagcagcacc gccggcaaac tgtcggcaga ccgcgcgcgt ggggccgggc
    22081 agatcgcccg cgggatgctg agtctcgcgg gcatctggcc ttccttcctg tctccgctgt
    22141 gggtggtgct ggcgctgttc ttcggtcgcg gcctgtcggc ccaaagcgag ggccgcgacc
    22201 cggtggtctg cgggctgttc gcgcgctatc tgctgctgct ggtggcgctg ctggcggcca
    22261 cggtgctgat cggcggcgcc tcgcatttca acgaccgctg gctgcagccg ctgatgttct
    22321 tcgccccgct gatgttcttc gtcggctggc ccggattgct gcgacacccg cgcctgcact
    22381 ggctggcctg ggccgccgcg gcgatggcgc tgctgacgct gacgctgctg tccttgcgcc
    22441 cctggtacga cggccggcgc ggccacgtcg gcgacctgaa cctgcccgtc cagggcatcg
    22501 cacgggccgt gcgggccgcc gggatcgagc ccgccacgat cgtctccagc aacaagcacc
    22561 tcgccggttc gctgcggctc gcgtttcctt cggcacgcat cattcactcc agcgcaaatt
    22621 cgccggtgct gcctgcaggg ccggtcctgc tgctcggttc ggcggccggt ttcgacgctc
    22681 tgcgcgctca gcaaccgggt tggaccgacg cgccggcgac caccatcgag gtgccctacg
    22741 cccatgcccg cgcagacgcg ccgaagctga aattcacctt cgcgcaggca ggcagcccgg
    22801 cgaattgacg gcgccttcag cggcgctgcg ggtggtagcg cggccggaac cagtcggtga
    22861 atcgctggat gccgtcggcc agcgaggtgg ccgggctgaa accgacccag gcgtccagcg
    22921 ccgacgtgtc ggcgtgcgtg gccagcacgt cgccgggctg cagcggcagc atctcgcgaa
    22981 cggccgtcac cccgagtgca cgttccatcg tgtcgatgaa ctcgagaagc ggcaccggcg
    23041 tgctgttgcc gatgttgaac agccgccagg gcgcgctgct ggtcgacgga tcgggctgtg
    23101 cggcgtcgta cagcgggtcg gctgtcgccg gcttgtcgag cacgcgcagc acgccttcga
    23161 cgatgtcgtc gatgtaggtg aagtcgcgcg tcatgcggcc ttcgttgtag accttgatcg
    23221 gacgcccgga gagcatcgcg tcggcaaaca ggaaaggcgc catgtccggc cggccccagg
    23281 gcccgtagac cgtgaagaag cgcagcccgg tggtcggcaa gccgtacagg tggctgtagc
    23341 tgtgagccat cgcctcgttg gctcgcttgg tcgccgcata caggctcacc ggatggtcga
    23401 cgctgtccgc ctcgctgtac ggcagcttcg cgttgccgcc gtagacgctg gacgagctgg
    23461 cataggccag atgctcgacg ccgccatggc ggcagccctc cagcacgttg gcaaagccga
    23521 ccaggttgct gtcgacgtag gccatcggat tcgtcagcga atagcgcacg ccggcctgcg
    23581 cgccgagatg cacgacacga tcgaagcgtt ctcgcctgaa cagcgaagcc atgccgtcgc
    23641 gatcggccag gtccatgcgc tcgaacacga aatcgggggc accgctgagg cgtgccaggc
    23701 ggtcggtctt cagtgtcggg tcgtagtagt cgttcaggtt gtcgatgccg accacgcgat
    23761 cgccccgagc cagcaggcgc tgcgcgacga acatcccgat gaagccggcg acgccggtga
    23821 cgaggacctt catgggtgcg gcgtgggcgc cacaatgcga gcgcgcagga tggaggaatt
    23881 catcgtgcca ttgtcctgtt gtttgcatgc ctctttccac ccccccatcg cttgcgatcc
    23941 tgtcccggct gaaccggccg ctggccgacc tgccgacgcg ctgtgacgtg atcgtcgtcg
    24001 gcgccggtcc tgccggcagc gcggcggcga ccaccctggc gcgcgcggga ctcgacgtgg
    24061 tgctggtcga ccagcaggcg ttcccgcgcg acaaggtctg cggcgacggg ctgatccccg
    24121 atgcgcaccg cgcgctgcaa cggctcgggc tgctcgacgc cgtgctggcg cgcgcgcaat
    24181 cggtgaagca tgtcggcgtg atcggcccgc ggggcggacg catcgacgtg ccgggcacgc
    24241 tggccgtgct gccacgccgc gagctcgacc tgctgctgtg ctcggcggcg gccgaggccg
    24301 gcgcgaggat gcacgcgccg gttcgcttcg ttggcgtgat cgaggaggat gagggccaga
    24361 ccacgcgcgt cgtcggagtc cggctgcgtt ccggtgacac cacacgcgaa ctgcgcgcgg
    24421 cctgggttct gattgccagc ggggcgccac cgcaggcgct gcaggcggcc ggcatgtgcg
    24481 agcgccgcac tcccaccggg gtcgccatgc gcggctacgt gaagaacccg gcgctggtcg
    24541 ggcgcatcac cgagctcgag gtggtgtggc acccgaggat gaagcgcggc tacggctgga
    24601 tatttccgtg tcccgacggc gtgttcaaca tcggcgtcgg cgtgacgcac agtcacaagg
    24661 cgggtcacgg tccgggccag tcgatgcatg acgtcaacct gcgcacgatg ttcgacgagt
    24721 tcacgcgggt ccatccggct gcgcgcgatc tgattgccgg cggcacctgg gtcggcgagc
    24781 tgaagggcgc gccgctgcgt tgctcgctgg ccggagcgcg gctgtcgcga cccggcctgc
    24841 tggtgaccgg tgaagccgcg ggcagcacct atgcgttcac cggcgaaggc atcggaaagg
    24901 cgctggagac cggcatcgcc gcagccgaga cgctgatcga agggcggcgc agcaactggc
    24961 ccgaggcgca ggtgcgcgcc acctatgaag atcgggtgca agcgctcaag ccgcgatacg
    25021 acatgtacga caaggcgggc atcgtcaacg cccacccgtg gctggtcgag ctggtggtgt
    25081 ggagcgcccg gcgcagcccg ggtcgcctgc gccgcatgac gggcctgctc gaggagacgc
    25141 acctgcccgg cagcctgctg accccgcgga gctggctgcg catgctgttc gttcactcct
    25201 gaaggcagac cgcatgtgcc agctcctcgg gatgaacggc aacacgccga ccgacatcgt
    25261 cttcagtttc accgggttct cgacccgcgc cgaggaacac caggacggtt tcgggatcgc
    25321 cttcttcgag ggtgccggct tgaggctgtt cgtcgatgcg cagagcgcgc gccactcgcc
    25381 ggtggccgat ctggtgcggc gctatccgat ccactcgaac aacgtcatcg cgcacatccg
    25441 caaggccacg cagggccgca tcgcgctgga gaacacgcac cccttcgtcc gcgagttgtg
    25501 gggtcgctac tgggccttcg cgcacaacgg caacctgacc ggttacgcgc cccggctgca
    25561 cgcgtccttc cacccggtgg gcgacacgga cagcgaacgc gctttctgct ggctgatgca
    25621 ggagctcgcc aaggcccacg ccagcctgcc gcccgtggcc gagctgaccg ccacgctgcg
    25681 cgaactggca ccgcaggtcg ccgcacacgg cagcttcaac ttcatgctga gccacggcga
    25741 ggcgctctgg gcgcactgct cgaccaggct gagctacctg gtccggcagc accccttcca
    25801 ggaagcccgg ctgcaggacg aggacatcag cgtcgatttc gcccggctga ccaccccgtc
    25861 ggaccgcgtg gcggtgatcg tcacggaacc gctgacccgc gacgaggcct gggtcgcctt
    25921 cgccccgggc gaactgaagg tcttcgtcgc aggcgagccg ctgtgcgcgt gacgaagagg
    25981 ctcctcgcgc gcctaagatc gtcggaacac tgcagcccgg caccttcatg accctcacgg
    26041 cctccacccc cgtcccgcgc ttcgacacct tctaccgcca cgacgcactg acgcagctgc
    26101 tcgccgacta cgccgcggca cacccggagc tggtgtcggt ggtctcgatc ggcaagagcc
    26161 acgagg
//
LOCUS       DAOORV010000042        25670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2290_length_25670_cov_7.348897, whole genome shotgun sequence.
ACCESSION   DAOORV010000042 DAOORV010000000
VERSION     DAOORV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 25670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_25670_cov_7.348897"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PKV56_07690"
     CDS             <1..1331
                     /locus_tag="PKV56_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOX67736.1"
                     /translation="GCKVAVVDATGKVLATETIYPLEPRRDWEGSLHTLGRLCATHGV
                     DLIAIGNGTGSRETDKLASELIQRLHKIAPDRTVIKVVVSEAGASVYSASEYASKELP
                     DLDVSLRGAVSIARRLQDPLAELVKIEPKSIGVGQYQHDVNQSDLAKSLNAVIEDCVN
                     SVGVDVNTASAPLLSRVSGLSGAVASGIVRWRDAHGAFRNRQQLLDVAGLGAKTFEQS
                     AGFLRVRDGDNPLDQSGVHPETYPVVERILAAVKRPAAEVMGRSDVIRALKPEAFADE
                     RFGAITVKDILAELEKPGRDPRPDFKVAKLTDGIEDIKDLQPGMTLEGTISNVAQFGA
                     FVDLGVHQDGLIHVSQLANKFVSDAREVVKTGDVVKVKVLEVDLSRQRISLTMKLDAV
                     PAERGAREGSGAGNRFQPAGRGDRPRSGGASSPPADGAMAAAFAKLQTRR"
     gene            1533..3212
                     /locus_tag="PKV56_07695"
     CDS             1533..3212
                     /locus_tag="PKV56_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HOX67737.1"
                     /translation="MNTTLLPSNRLLPVIGALAATCGLACVLPAHASSHREAPSITST
                     PKVDATDFYMFNSYEAGRGAYVSLMANYVPLQDPYGGPNYFSLDPNALYEIHIDNNGN
                     GVEDITFQFRFKNTLKGITLPIGSANVAIPLIQSGGVSGPAAAALNLNETFTVEIVRG
                     DRRKGSKASITNAAGGSATFNKPVDNIGSKTIGDYNAYAAQHVYAVNIPGCAVPGRLF
                     VGQRKDPFAVNLGTIFDLVNAPLAVVSNPANIGAAGAGDLADKNVTTLAIEVAKDCLT
                     ARPSSDSTYDPVIGGWTTASLRQGSLLNPNPKSGHGTASISGGAWTQVSRLGSPLVNE
                     VVIGLPDKDRFNASKPSGDLQFATYVTNPTLPALLGVALAGDIAAVAPTNLPRTDLLN
                     VFLTGLAGVNKPKGTVTPAEMLRLNTAIPAVPFASQNRLGVAGELLRVGGAANLGSAV
                     DLAGYPNGRRPKDDVVDISLIAVAGALCVANSNGVIANLGAADNNNVLGLNSFTIPGS
                     TPTVLSSECRLGKVPLGATSTALHDAVDQAAVTFSDSFPYFTKPLPGAGSN"
     gene            3223..3483
                     /locus_tag="PKV56_07700"
     CDS             3223..3483
                     /locus_tag="PKV56_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67738.1"
                     /translation="MTKQSIFLGSCVALLLSACGGGGGGDSTPPVTEAVPSAAAADSE
                     AATQYLVQLTETAPASTDRIEPITDLPDKLAADDTAEPRSVP"
     gene            3576..4745
                     /locus_tag="PKV56_07705"
     CDS             3576..4745
                     /locus_tag="PKV56_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67739.1"
                     /translation="MSGRSAVHAAWWFAIGVAAWASPVGGWAEVVSPTRDSEVVEVLP
                     SGGAGAEERRLRRLWVNQPTDAVTAVSLARRYLSRAREQGDPRHAGQALAVLQHWPDA
                     RTAPDEVLLLQATLQQYLHEFDVSAGHLELLVARRPEHAQAWLTLATVRRVQGRYADS
                     DAACRGLQAAGVRLESQACLAENQSLRGDFDAARNSLRQLLAAPRLPASTRSWLLTTL
                     AEVEQRAGRPDIAESTFKQALAVESGGYTLLTYTDFLMDLQRDAEALALLTPQPRTDA
                     VLLRLAIAGMRLKSPGAAADARQVRDTIAQANLRPDARGTHAREQAMFALEVEAMPVA
                     ALVLARENVRRQREPLDLLVLARAARAAGDQIAIREVDQLRREMGLRDRRLDALL"
     gene            4750..5988
                     /locus_tag="PKV56_07710"
     CDS             4750..5988
                     /locus_tag="PKV56_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HOX67740.1"
                     /translation="MRAARHGMLLCCVWLAAPALAHKGSDGYIQLKLDATASVLRVDL
                     ALRDLDAVLDIDADADGNLTWGEIRATWPAIDRYISARVQVDGCRWTSAARALERRAD
                     GVYAALTWSGSCTSAAVPPVIRYVALREIDPTHRAIAKIERPGSEAEVRVLDPTRSIA
                     PISAVESSADRSGAIDSILSPVAAGNPVAASAGVASAVAGVSESPQVPGPATAGFVEE
                     GVRHILTGYDHMLFLLCLLLPAVMRRTPSGWQPVERLSQAVVPVAVVVTAFTVAHSVT
                     LTLAGLKLLSLPSSFIEPAIAVTIILAAIDNVRPIFRGRRALVTFCFGLIHGFGFAGV
                     LGELDLPAGQFAWALFQFNLGIEMGQLFVVSIAVSCLFLFRRNGLYPRWIIQGGSAAA
                     ICVGALWLIERSADVSIPGF"
     gene            6179..8098
                     /gene="thrS"
                     /locus_tag="PKV56_07715"
     CDS             6179..8098
                     /gene="thrS"
                     /locus_tag="PKV56_07715"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOX67741.1"
                     /translation="MISIQLPDGSKREFPGPVTVAEVAASIGAGLAKAALAGRVGSGT
                     ESRVVDTSHLIDRDTVLAIVTDKDADGLEVIRHSTAHLLAYAVKELFPDAQVTIGPVI
                     ENGFFYDFSYKRPFTPEDLAAIESRMTELAKKDEKVERRVLPRDEAVAYFKGIGEAYK
                     AEIIASIPADQDVSLYREGKFEDLCRGPHVPSTGKLRHFKLMKVAGAYWRGDHRNEML
                     QRIYGTAWATKDELQQHLHMLEEAEKRDHRKLGRELDLFHIDEHAPGLVFWHPKGWTI
                     WQQVEQYMRSVYRNNGYQEVKGPQVLDQGLWEKTGHWDKYRENMFTTESEKREYALKP
                     MNCPGHILIFRQGIKSYRDLPLRLGEFGQCHRNEPSGSLHGIMRVRGFTQDDGHIFCT
                     EDQILDECTAFTTLLQKVYADFGFTDIIYKVATRPEKRIGSDALWDKAEFALMESLRR
                     SGCEFVVTPGEGAFYGPKIEYTLKDAIGRQWQCGTIQVDPNMPERLGAEYVTESGERA
                     TPMVLHRAIVGSLERFIGILLEQHAGALPAWLAPVQVVVTPISDGQADYARDAAKALQ
                     KQGVRVVTDLRNEKITYKIREHSMQKVPYILVVGDKEKANGAVAVRARGNQDLGVMSL
                     EDFSSKLAGDIAQKA"
     gene            8269..8751
                     /gene="infC"
                     /locus_tag="PKV56_07720"
     CDS             8269..8751
                     /gene="infC"
                     /locus_tag="PKV56_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOX67742.1"
                     /translation="MATEVRLNGVENEPLGIVSTLEALRMAGELDVDLVEIAATAEPP
                     VCRLMDYGKFKYQEQKRAAEAKSKQKVIEVKEVKFRPGTDEGDYQIKMRNLRRFIEED
                     GDKGKVTLRYRGREITHQDIGMRLLERIRDELADVSVVEHMPKLEGRQMIMVLAPKRK
                     "
     gene            8871..9074
                     /gene="rpmI"
                     /locus_tag="PKV56_07725"
     CDS             8871..9074
                     /gene="rpmI"
                     /locus_tag="PKV56_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOX67743.1"
                     /translation="MPKMKTKKSAAKRFRVRPGGTVKRGQAFKRHILTKKSTKVKRHL
                     RGATAVHETDTGRIKQMMPFAGL"
     gene            9095..9451
                     /gene="rplT"
                     /locus_tag="PKV56_07730"
     CDS             9095..9451
                     /gene="rplT"
                     /locus_tag="PKV56_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOX67744.1"
                     /translation="MPRVKRGVTARARHKKIIALAKGYRGRRKNVFRIAKQAVMRAGQ
                     YAYRDRRTKKRVFRNLWIARINAGSRSHGVTYSKFMAGLKKLSIEIDRKVLADMAVHD
                     PVAFGSIVAKVKAQLA"
     gene            9595..10638
                     /gene="pheS"
                     /locus_tag="PKV56_07735"
     CDS             9595..10638
                     /gene="pheS"
                     /locus_tag="PKV56_07735"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOX67745.1"
                     /translation="MNDLEPLVQEAQRDFAQAATPADLENAKARYLGKSGRLTDQLKA
                     LAALSADDKKARGALINAAKQQVEAALNARRQALADAELDAQLQSEALDVTLPGRHRG
                     TGGLHPVSRTMERIEAIFGSMGFDVAEGPEIETDWYSFTALNNPENHPARSMQDTFYV
                     DMKDAEGRWLNLRPHTSPMQVRYARAHAEKHRGQDPMPEIRVIAPGRTYRVDSDATHS
                     PMFHQCEGLWIGENVSFKDLKVVFTDFFRTFFETDDLQLRFRPSFFPFTEPSAEVDIA
                     FASGPLKGRWLEVAGSGQVHPNVVRNYGLDPERHLGFAFGMGPDRLTMLRYGVSDLRL
                     FFDGDLRFLSQFK"
     gene            10675..13101
                     /gene="pheT"
                     /locus_tag="PKV56_07740"
     CDS             10675..13101
                     /gene="pheT"
                     /locus_tag="PKV56_07740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOX67746.1"
                     /translation="MQFPESWLREFCNPPLNTAELADLLTMAGMEVEDLRLAAPPFTG
                     VVVGEVLSVERHPDADRLNVCQVDVGAASPLNIVCGAPNVRAGIKVPCAMVGAELPPA
                     DDKISVEPFRIKLGKLRGVESQGMLCSARELKLSDEASGLLVLAPDALVGCSIREHLL
                     LDDTLFTLKLTPNLGHVLSVYGVAREVAALTGSPLLQPMFPKARVESTHVLKVSIDAP
                     DLCGRFSGRIVRQVNPKATTPAWMVDRLARCGQRSVSPLVDISNYVMFEFGRPSHIFD
                     LDKIHGSLQVRWGRAGEALKLLNGNTIEVDEKVGVIADEQAVESLAGIMGGDATAVSD
                     DTRNIYVEAAFWWPEAVAGRSRRYNFSTDAGHRFERGVDPATTVEHIERLTQLITDIC
                     GTPGTVCGPIDDQVLSLPAATPVALRIERAVKVIGMPLTQAQCAGVMQRLGLACTETP
                     GVLTVTPPSWRFDLQIEEDLIEEVIRVIGFNTLPDTAPRAPVTARVRPEAQRSPHAVR
                     RQLAALDYFETINFSFVEERWERELSGNADPIRVLNPIASPLSVMRSSLIGSLVNTLR
                     YNLARKAPRVRLFEVGRVFRRDAAAVDAPLGVAGVAQPMRVAGLAYGPVDAAQWGNKD
                     RTVDFFDVKGDVQALFAPRMVHFSSCEHPALHPGRSARIEVDGAVLGHIGELHPRWRQ
                     AYELPGAPVVFEIDLAALLRTTVPAFAPLPKQQSVWRDIAVMADESVTHEALMQSILE
                     VPGGLIRSANLFDVFRPPSASAAASERSLAVRLELLDDDATFTDERIDAAVARVLETL
                     RTRLGVRLRG"
     gene            13107..13466
                     /locus_tag="PKV56_07745"
     CDS             13107..13466
                     /locus_tag="PKV56_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOX67747.1"
                     /translation="MNDDSKPVPLMLSSLDTPTLTKAELADLLFERLGLNKRESKDMV
                     EAFFDIIHDALITGRDVKMSGFGNFNIRRKAPRPGRNPRTGEEIPIKARNVVTFHASH
                     KLKDVVQGDTPAPDDFE"
     gene            13501..13983
                     /locus_tag="PKV56_07750"
     CDS             13501..13983
                     /locus_tag="PKV56_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX67748.1"
                     /translation="MENSLPAIPAKRYFTIGEVSDLCGVKPYVLRYWEQEFTQLKPMK
                     RRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNRLIEPSSGALTAAAAAGPEDEES
                     TVAMNASGGAAEAVQAVLDRVDLSPSVASTTRPMAIGSAMSNAQMRSELLSIRTLLTP
                     "
     gene            14018..14094
                     /locus_tag="PKV56_07755"
     tRNA            14018..14094
                     /locus_tag="PKV56_07755"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14052..14054,aa:Pro,seq:ggg)
     gene            complement(14294..15499)
                     /locus_tag="PKV56_07760"
     CDS             complement(14294..15499)
                     /locus_tag="PKV56_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX67749.1"
                     /translation="MSLLDKFAAQRALQATLAASSTVPPIATPMDAVHSATSATINNR
                     PVILAGTNNYLGLTYDPECRAAAVEAIESLGTGTTGSRMASGNYAGHRALEQALANAF
                     GWPACIVFSTGYQANLGAISALASEGDFLLVDADSHASIHDGCRLSSATTIRFRHNDP
                     ENLDRRLERLGEDASRTLIVVESLYSTLGDRAPLREIVEIKRRHGAWLLVDEAHSFGV
                     FGPRGLGICEELGLLDEVDFIVGTFSKSLGSIGGFCLGRHPETELIRMVSRPYIFTAS
                     PAPPVIAATHQALRRVLQGQDLRARLFSHAERLYAEAARLGYRLGTTAPGPVAALVFE
                     TRDAALALWQALIDAGIYTNLMIPPATPSGLCLVRISLSAAHSDDDITQIIAALAAHA
                     QRSRSPLAA"
     gene            complement(15496..15750)
                     /locus_tag="PKV56_07765"
     CDS             complement(15496..15750)
                     /locus_tag="PKV56_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX67750.1"
                     /translation="MSDASASIESTIREALATIRPEAADLPGDADLAQAADLDSADVM
                     DLVMEIEDRLDISIPVETMAQARTINELCAGIARLTRNVT"
     gene            complement(15786..17726)
                     /locus_tag="PKV56_07770"
     CDS             complement(15786..17726)
                     /locus_tag="PKV56_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HOX67751.1"
                     /translation="MQRGGAQITFPGSSSFAEVLQNKKGGGVDSSLARPVRPTGVRPV
                     NGATPSLYSPTPTASGIELRSADFATLAEALDYAAQGESGFNYYDGRGKLIATLSYRE
                     IRSLALEMARHLAPLGRGQRMALVAHTHVDFAVMFYACQYAGLVPVPLPAAVHLGGRE
                     AYIRHLRQLLRDCKAVAAFAPPEFIGFLREASEGLPMALAGTLEEFSDLPALDTPPPP
                     PTPSELAYLQYTSGSTRFPRGTMITQSAVMANLKAIFNHGFRLTPGDRFCSWLPYYHD
                     MGLVGIVLGCVATQRSVDYLPTRDFAMRPRLWLKLMSQNRSTISFSPPFGYSLCARRL
                     RASDTQELDLSAWRVAGIGAEMIHPESLVEFADSLESAGFDRRAFLPCYGMAECSLAV
                     SFAPLEGGARSDVVDMDRLAHDGEAIPVVADGAMPKGKAFIDCGPPLPGYEMEIRDEQ
                     GRPLPERRCGRIHLRGPSVMSGYFGNAEATREVLSADGWLDTGDLGYQADGTLHITGR
                     AKDLMIIMGRNIWPQDLEHLAEQQPEVRHTDASAFSIPDASEGEMAVLVVQCREADPR
                     KLAGLAERLQQAIHLEFGIHCLIELVPLHTLPRTSSGKLSRSTARKEFLERRSAAAPQ
                     ESRRRFVGDSRRARVEAQSAAS"
     gene            complement(17829..19790)
                     /locus_tag="PKV56_07775"
     CDS             complement(17829..19790)
                     /locus_tag="PKV56_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HOX67752.1"
                     /translation="MPQFETLDAFLPADAREDRQITFIEGEHDQRTLTFRRLRQRAIG
                     ALGSLQRRGFSRGDMLILYLGDNERFVEMFWACLLGGIVPVPLAPGGNDEHWRKLLRV
                     FAQLDRVSVCLDAPALERFDAFVRRHDLKAEGDLLRSRTVMVGSLDIAGEPGLPFQAQ
                     PDDIAFIQYSSGSTGEPKGVLLTHRNLTANAASIIEGAAYTDRDLVLSWMPLSHDMGL
                     IGFHLTFLAGGISHTIMRTDLFARRPLLWMELASQQRSTVLCSPNFGFQHYLRQYDIK
                     SPQGLDLSSIRLIFNGAEPISADLCRRFMRTMAPHGLKPNTLFPVYGLAEATLAVSFS
                     RPGAPLETVKLSPAILRVGEQVDTLSADTGRLIEFVKVGPPLPGNEVRIVDDDGVTLE
                     ERMLGHVQMRGDNVTQGYYRNEKRTAEVRSADGWLDTGDLGLLWDGQLVITGRAKDLI
                     IINGLNHYPQDLERIAEQVEGIEPNRVAAAGVRRTGAETEELAIFVLHRGDIADFPLK
                     ALALRKAILQQSGLEATHVVPVRQIPKTSSGKLQRYALAEALERGEFDSVLTELAPLL
                     AASSAADPREYETSPTVGRLLAICAGFIPDRTLSPHTNLLEINLNSLTLARIHEAIER
                     EFPQRIEVTDLFDYPTLHQLADFLDSTSS"
     gene            complement(19801..21111)
                     /locus_tag="PKV56_07780"
     CDS             complement(19801..21111)
                     /locus_tag="PKV56_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOX67753.1"
                     /translation="MVQELLAPGPQERHDLDADDESLLLLQELKPRFGDICRVTACSR
                     ASDSLVIHSPDDIRHVLLVNRGNYVKGVGLERVRVLLGKGLIANDGESWTRQRRMMQP
                     MFHSQVIRGFSIFMERLNLDLIERWAGHAARCEPINLTRDLSEVTLGIVLRALFGSDL
                     DRLIASEGSSPFDLLTRETRRDLPFAAKFRALTRHVIAMIDARRKERRIEMDLLSMLM
                     EARDRDSGEAMPDRAILDEIMTLIVAGHETTASTLNWTWYLLSQHADVESRLHESIAD
                     AIPLPAPAQSAGDEPPQARSYVERVLQESMRLYPSGWLLTRRALRDDRLAGYQVPAGT
                     DIFICPYLLHRDARHWEHPDDFDPDRFLTDAAESRHRFAFLPFSAGPRYCIGASFAMT
                     EMTMHLSMVAQRFRLRYAASKPPQAEFQINLRTRQDVYMHVVAR"
     gene            21174..21875
                     /locus_tag="PKV56_07785"
     CDS             21174..21875
                     /locus_tag="PKV56_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOX67754.1"
                     /translation="MSVTVFITSEFSPTPAIEAEWLAQLPLRRRSEIAGWSDQRARRQ
                     SLLGSRLLSAGLTRLGFEAEGLDSLSYGPRSRPTLDLPVEFSLSHCEGRVVCALSTCG
                     PVGIDVEALGDLTAEDFRIYLSPAERSWAARSARRFYSVWTRKEAVAKAAGHRGLRDV
                     ARVDTGAAEQRAALDGCLWRTWPIPAGRRHMAHLAMADQPCKVTVRRLARDEIEHGLR
                     PAARVRPVASYQAVL"
     gene            21916..22701
                     /locus_tag="PKV56_07790"
     CDS             21916..22701
                     /locus_tag="PKV56_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOX67755.1"
                     /translation="MSQPQRAVILSAGQGSRLLPLTAQMPKCLLDLCGRSMLEWQLRG
                     LSQMGVPEAVVVTGFHPELVEAELKRVAPQNMRVRTLYNPFYKLADNLASCWMARAEL
                     TGTCLILNGDTLFEPDIAGRLIAAASAPITVTIDRKPNYDSDDMKVETDGVHLRAIGK
                     TLSAERVNGESIGFLRFEPEGAALFVEELERTMRTPEGPGKWYLSAINRLAESGADIR
                     VASIEGLEWGELDFPDDLIQVRALAAGWLEQERASSRGASATA"
     gene            complement(22730..23539)
                     /locus_tag="PKV56_07795"
     CDS             complement(22730..23539)
                     /locus_tag="PKV56_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX67756.1"
                     /translation="MRQLTALVLAGTRAGGDPLAAYAGVSHKALIEVGGTTMIERVVS
                     TLAAAPDVARIIVAIERPDLLIGLPGLQPPLCCKPVSTMPTGSGPSATVAAALVREGT
                     PLLVTTADHALLEAAWVQEFLAACPADADVVLGLAERAAVEAAAPDTRRTWLRFSDGD
                     FSGCNLFLMASPAAAGAVTLWRELERERKAPLRMMRRLGWTIAMRYRLGWLSSTAATA
                     RLGSLAGGARITIAQMSDGRAAIDVDKPEDLDLVRRLVEVRREGICSGSAN"
     gene            complement(23583..24686)
                     /locus_tag="PKV56_07800"
     CDS             complement(23583..24686)
                     /locus_tag="PKV56_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP pyrophosphohydrolase"
                     /protein_id="HOX67757.1"
                     /translation="MGSPAELDRFIKLPARLYAGDPAFVAPLVMERREALSPTKNPYF
                     AHAETQFWLARRDGRDVGRISAQIDRLVTDPDVGHFGMIVAEDDAEVFAALFAAAEGW
                     LRARGKRRIMGPFNLSINEETGLLIDGFDAPPVMLMGHDPRYAGPRIEALGYAKAKDL
                     IAYMYDIDHELPAAARRKIDSRKATALTVRRLDMQRYREEFDTVTAIFNDAWSQNWGF
                     IAFTEAEIAHMAKSLKMLIDPNCVAIVEMNGEAIGFGIALPNLNELIADFGGNLLPFN
                     WIKLMYRLRRGARTARVPLMGIRRSCSTSFAGGLAPFLVIDALRKGLRAKGVKQVELS
                     WILEDNRPMRHVIEALGANPYKTYRVYEKTLAQ"
     gene            complement(24710..>25670)
                     /locus_tag="PKV56_07805"
     CDS             complement(24710..>25670)
                     /locus_tag="PKV56_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOX67758.1"
                     /translation="IDPRLIAFLVRRDGSCAAARGEGDHWAVALRLAPELADAIPEQA
                     EDLRTVAETLMAAGLIAPFDEKEFPAYMDKLRRSLPYWIHAVTDTVTRQRLERQMFWD
                     NYKGSTDLLTRYVYPPLVWPLVRLCVRLGLHPNTVTLLSIVLTIAAVPLFARGDFLAG
                     FVCAYGMSVLDSVDGKLARLTLTDSKIGNLLDHGTDIVHPPFWYFAFAWGLGGHNPGD
                     PLYQAAIWMIVFYVADRVVLSIAKRRIGFALHAASRLDGFVRSFIARRNITMTIMAFG
                     VLLGVGPAAFYVITAWQGLTFAWHAARTVWHGFLSRAQARPAA"
BASE COUNT         4558 a         8106 c         8446 g         4560 t
ORIGIN      
        1 ccggctgcaa ggtcgcggtg gtcgatgcca ccggcaaggt gctggccacc gaaaccatct
       61 atccactgga gccgcgtcgc gactgggagg gttcgctgca caccctcggt cggctgtgcg
      121 cgacgcatgg cgtcgacctg atcgcgatcg gcaacggcac cggcagccgc gaaaccgaca
      181 agctggcgtc cgagttgatc cagcggctgc acaagatcgc cccggatcgc acggtgatca
      241 aggtggttgt cagcgaagcg ggcgcctcgg tctacagcgc cagcgaatac gccagcaagg
      301 agctgccgga tctcgacgtg agcctgcgcg gcgcggtcag catcgcgcgg cgattgcagg
      361 atccactggc cgaactggtg aagatcgagc cgaagagcat cggcgtgggc cagtaccagc
      421 acgacgtcaa ccagagcgac ctggcgaaga gcctgaatgc ggtcatcgag gactgcgtga
      481 actcggtcgg cgtcgatgtg aacacggcgt cggcgccgct gctgtcgcgc gtgtccggac
      541 tcagcggtgc ggtcgcttcc ggcatcgtgc gctggcgcga tgcgcacggg gcctttcgca
      601 accggcagca actgctcgac gtggccggcc tcggcgccaa gacgttcgag cagagcgcgg
      661 gtttcctgcg agtgcgcgac ggcgacaacc cgctggacca gtcgggcgtc cacccggaga
      721 cctacccggt ggtcgaacgc atcctggccg cggtgaagag gccggccgct gaggtgatgg
      781 gccgcagcga cgtgatacgc gcgttgaagc ccgaggcgtt cgccgacgag cgattcggcg
      841 cgatcaccgt caaggacatc ctggccgagc tcgaaaagcc gggacgcgac ccgcgccccg
      901 atttcaaggt ggcgaagctg accgacggga tcgaggacat caaggacctg cagccgggca
      961 tgacgctgga gggcacgatc agcaacgttg cgcagttcgg cgctttcgtc gatctgggtg
     1021 tccatcagga cgggttgatc cacgtgagcc agctcgcgaa caagttcgtg agcgacgcgc
     1081 gtgaggtcgt gaagaccggt gacgtggtca aggtcaaggt gctcgaggtc gatctgtctc
     1141 gccagcggat ctcgctgacg atgaaactcg atgcggtgcc cgccgagcga ggcgccaggg
     1201 agggctccgg tgccggcaac cgtttccagc ctgcaggacg cggtgaccgg cctcgttcag
     1261 ggggggcgtc atcgccaccg gctgatggcg cgatggcagc ggccttcgcc aagttgcaga
     1321 cgcgacgctg agctgcggtt gcttctgaag agcgtccagg gttgacggcg agacattgac
     1381 cgttgtctgg cgcccgcagg gtgcatccag aaacgcgatt cgctcgtatg gggtcggtag
     1441 cgcgatccgg cggccctccg tcggtgcgcc tcgccgcagc gcgacacatc ggtgtggcga
     1501 ggcgaagtcc acaaccccac gaggctcgaa ccatgaatac aaccctgctg ccatccaacc
     1561 gcctgctgcc ggtcatcggc gcgctggccg cgacctgcgg cctggcctgt gtgctcccgg
     1621 cgcacgcgtc cagtcaccgt gaagcgccgt cgatcacgtc cacgcccaag gtggatgcga
     1681 ccgatttcta catgttcaac agctacgagg ccggacgcgg cgcctatgtg tcgctgatgg
     1741 ccaactacgt gccgctgcag gatccgtacg gcggcccgaa ctatttctcg ctcgacccga
     1801 atgcgcttta cgagatccac atcgacaaca acggcaacgg ggtcgaggac atcactttcc
     1861 agttccgatt caagaacacc ctgaaaggca tcacgctgcc gatcggcagc gccaatgtgg
     1921 cgatcccgct gatccagtcc ggcggtgtgt cgggcccggc cgcggccgcg ctgaacctca
     1981 acgagacgtt caccgtcgag atcgtgcgcg gcgaccgccg caagggcagc aaggcgtcga
     2041 tcaccaatgc ggctggcggc tcggccacct tcaacaagcc ggtcgacaac atcggctcca
     2101 agacgatcgg cgattacaac gcctatgcgg ctcagcatgt ctacgcggtc aacatccccg
     2161 gctgtgctgt gccggggagg ttgttcgtgg gccagcgcaa ggacccgttc gcggtcaacc
     2221 tgggcacgat cttcgatctg gtgaatgcgc cactcgccgt cgtcagcaac cccgccaaca
     2281 tcggcgcggc tggtgctgga gacctggccg acaagaacgt cacgacgctg gccatcgagg
     2341 tggccaagga ctgcctgacc gccaggccgt ccagcgacag cacgtacgac ccggtgatcg
     2401 gtggatggac caccgccagc ctgcgccagg gcagtctgtt gaacccgaat ccgaaatccg
     2461 gccatggcac cgcctccatt tcggggggag cctggacgca ggtctcgcgg ctgggcagcc
     2521 cgctggtcaa cgaggtggtg atcggtcttc ccgacaagga caggttcaac gcctcgaagc
     2581 cttcgggcga tctgcagttc gcgacctatg tgaccaaccc gacactgccg gcactgctgg
     2641 gcgttgcact ggcaggcgac atcgctgcgg tggcgccgac caacctgcca cgcaccgatc
     2701 tgctcaacgt gttcctgacc ggtctggccg gcgtcaacaa accgaagggc acggtgaccc
     2761 cggctgaaat gctgcgcctg aataccgcga ttcccgcggt gccgttcgcg agccagaacc
     2821 gcctgggtgt ggcgggtgaa ctgctgcgcg ttggcggcgc ggccaacctc ggatctgcgg
     2881 tcgacctggc cggttacccc aacggtcgcc gaccgaagga tgacgtggtc gacatctcat
     2941 tgatcgcggt ggctggcgcg ctgtgcgtgg ccaacagcaa cggcgtgatc gccaatctgg
     3001 gggcagcgga caacaacaac gttctgggac tgaacagctt cacgattccg ggctccacgc
     3061 caacggtact gtcgtccgaa tgccgccttg gcaaggtgcc gctgggtgcc acgtcgaccg
     3121 cactgcacga tgcggtggat caggcggcgg tcacgttcag cgattcgttc ccgtacttca
     3181 cgaagccgct gcctggcgcc ggctcgaact gaggggagca tcatgaccaa gcaatcgata
     3241 ttcttgggca gttgtgtggc cctgctgctg tcggcttgcg gcggtggagg tggcggtgac
     3301 agcacgccgc ccgtcaccga agcggtgccc tccgcggctg cggccgacag cgaggccgcc
     3361 acgcagtacc tggtccagtt gacggagacg gcgccggcca gtactgaccg gatcgaaccg
     3421 atcaccgact tgcctgacaa actcgccgcc gacgacactg cagagcccag gtcggttccg
     3481 taacgccatc cgttggcgag accgtcgacg acgcggcgcc ctggccgaca ttgcagggcg
     3541 ccggtttcgt cggcaactca ggtggagcac gaatgatgag cggtaggtct gcagttcatg
     3601 ccgcgtggtg gtttgccatc ggtgtggcgg cgtgggcatc tcctgttggc gggtgggccg
     3661 aagtggtgag tccgacgcgc gacagcgaag tcgtcgaggt gctgccgtcc ggtggtgccg
     3721 gtgccgaaga gcggcgattg cggcggctgt gggtgaacca gcccaccgac gcggtgaccg
     3781 cggtttcgct ggcccggcgc tacctttcgc gggcgcgcga gcagggcgat ccgcgccatg
     3841 ccggtcaggc gctggcggtg ctgcagcact ggcccgacgc gcgaacggcg cccgacgagg
     3901 tgctgctgct gcaggccacg ctgcagcagt acctgcacga gttcgatgtc tcggctgggc
     3961 acctggaact gctggtcgcg cgccgccccg aacacgcgca agcctggctg acgctggcga
     4021 cggtgcggcg tgtccagggc cgctacgccg actccgacgc tgcgtgccgg ggcctgcagg
     4081 ccgcaggcgt gcgactcgag agccaggcct gcctggccga gaaccagagc ttgcgcggcg
     4141 acttcgatgc cgcacgcaac agcctgcggc agctgctcgc cgcgccgcgg ttgccggcct
     4201 cgacccgctc ttggctgctg accacgctgg ccgaagtcga gcagcgggcc ggaaggccag
     4261 acatcgccga gtcgacgttc aagcaggcct tggcggtgga gtcgggcggc tacacgctgc
     4321 tgacctacac ggatttcctc atggacctgc agcgcgatgc cgaggcgctg gcactgctga
     4381 cgccgcagcc gcgcacggat gccgtgctgc tgcgcctggc gattgccggc atgcgcctga
     4441 aatcgcccgg tgccgccgcg gacgcgcgcc aggtgcgcga cacgatcgcg caggccaacc
     4501 tgagacccga tgcgcgggga acacacgctc gtgaacaggc gatgttcgcg ctggaggtcg
     4561 aggccatgcc ggtagcagcg ctggtgctgg cccgcgagaa cgtgcgcagg cagcgtgaac
     4621 cgctggatct gctggtgctg gcgcgtgctg ctcgcgctgc cggcgaccag atcgcgatcc
     4681 gcgaagtcga tcagcttcgc agggaaatgg gtcttcgtga tcggcgcctc gatgcattgc
     4741 tctgacttca tgcgagcagc ccgccacggg atgctgctgt gttgcgtctg gctggccgcc
     4801 ccggcactcg ctcacaaggg cagcgacggc tacatccagc tgaagctcga tgccacggcc
     4861 tcggtgttgc gtgtcgatct cgcgctgcgc gatctcgatg cggtactcga catcgatgcg
     4921 gatgcggacg gcaatctgac ttggggcgag atccgtgcga cgtggcccgc catcgaccgc
     4981 tacatctctg cccgcgtgca ggtcgatggc tgtcggtgga cgtcggccgc gcgagcgctc
     5041 gaacgtcgcg ccgacggggt ctatgcggca ttgacctggt cggggtcctg cacatctgct
     5101 gccgtgccgc cggtgatccg ctatgtcgcg ctgcgcgaga tcgacccgac gcaccgcgcc
     5161 atcgcgaaga tcgagcgacc gggttctgaa gccgaggtgc gggtgctcga cccgacgcga
     5221 tcgatcgctc cgatatccgc ggtcgaatct tcggctgacc gctcgggggc gatcgactcg
     5281 atcctttcgc cggtagccgc gggcaacccg gtggctgcaa gcgcaggggt tgcctctgcc
     5341 gtggcaggag tgtccgaatc gccccaggtc cccggtcctg caacggcggg gttcgtcgag
     5401 gagggcgtgc gtcacatcct gaccggctac gaccacatgc tgttcctgct gtgcctgctg
     5461 ctgcctgcgg tgatgcgacg cacgccgtcg ggctggcagc cggtcgaacg actgagccag
     5521 gccgtcgtgc cggtggccgt ggtcgtcacc gcgttcacgg tggcgcactc cgtgacgctg
     5581 accctggcgg gtctgaagct tttgtcgctg ccgtcgtcgt tcatcgagcc ggcgatcgcg
     5641 gtgacgatca tcctggctgc catcgacaat gtgcggccca tctttcgtgg acgccgggcc
     5701 ctcgtgacct tctgcttcgg gctgatacac gggttcggct ttgccggtgt gctcggcgaa
     5761 ctggacctgc cggccgggca gtttgcctgg gcgctgttcc agttcaacct gggcatcgag
     5821 atgggccagt tgttcgtggt ttcgatcgcc gtgagttgcc tgttcctgtt ccgccggaac
     5881 gggctgtacc cgcgctggat catccagggc ggatcggcgg cggcgatctg cgtcggggcg
     5941 ctgtggctga tcgaacgcag cgctgacgtg tcgataccgg gcttctgagt cgagtcgatc
     6001 tcgatgaatc acgcccggaa ccccgggcgc agccaatcgc cgtggcctaa aatgcagggt
     6061 tgacttcgac atcgaagcgc ggttcggcac cgcgcttttt tgtttcgagt gccgccgtcg
     6121 atcgggtcgg tttcggccag tccgacccga cggcgtcctg cgtttccctg gagctttcat
     6181 gatctcgatt caattgcccg acggttccaa gcgcgaattt cctggcccgg tgacggtggc
     6241 cgaggtcgcc gcgtcgatcg gcgccggcct tgccaaggcc gcgcttgcgg ggcgtgtcgg
     6301 cagcggtacc gagtctcgtg tggtcgacac cagtcacctg atcgatcgcg acacggtgct
     6361 cgcgatcgtc accgacaagg atgccgatgg cctcgaggtg attcgtcatt cgacggcgca
     6421 cttgctggcg tacgcggtga aggaactgtt cccggacgcg caggtgacca tcggcccggt
     6481 gatcgagaac ggtttcttct acgatttctc gtacaagcgc ccattcacgc ccgaggacct
     6541 ggcggcgatc gagtccagga tgaccgaact cgcgaagaag gacgagaagg tcgagcgccg
     6601 cgtgctgccg cgtgacgagg ccgtggcgta cttcaagggg atcggcgagg cctacaaggc
     6661 cgagatcatc gcgagcattc cggccgatca ggatgtgtcg ctgtaccgcg agggcaagtt
     6721 cgaggacctg tgccgcggtc cccatgtgcc cagcaccggc aagctcaggc acttcaagct
     6781 gatgaaggtg gcaggagcct actggcgcgg cgaccaccgc aacgagatgc tccagcgcat
     6841 ctacggcacg gcctgggcga ccaaggacga actgcagcag cacctgcaca tgctcgaaga
     6901 ggcagagaag cgcgaccacc gcaagctcgg acgcgaactc gatctgtttc acatcgacga
     6961 gcacgcaccg ggtctggtgt tctggcaccc gaagggctgg acgatctggc agcaggtcga
     7021 gcagtacatg cgctcggtct atcgcaacaa cggctaccag gaggtcaagg ggccgcaggt
     7081 gctcgaccag ggcttgtggg agaagaccgg ccactgggac aagtaccgcg agaacatgtt
     7141 caccaccgag agcgagaagc gcgagtacgc gctcaagccg atgaactgcc cggggcacat
     7201 cctgatcttc cgacagggca tcaagagcta ccgcgacctg ccgctgcgcc ttggcgaatt
     7261 cggtcaatgc caccgcaacg aacccagcgg gtcgctgcac gggatcatgc gggtgcgcgg
     7321 cttcacgcag gacgacggcc acatcttctg caccgaggat cagatcctcg acgaatgcac
     7381 cgcgttcacc acgctgctgc agaaggtcta tgccgacttc ggcttcacgg acatcatcta
     7441 caaggtggcg acccggcccg agaagcggat cggatcggat gcgctctggg acaaggccga
     7501 gttcgcgctg atggaaagcc tgcgccgttc aggctgcgag ttcgtcgtca ccccgggcga
     7561 aggtgcgttc tacgggccga agatcgagta cacgctgaag gacgcgatcg gccgtcagtg
     7621 gcagtgcggc acgatccagg tcgaccccaa catgcccgag cgcctcggtg ccgaatacgt
     7681 caccgagtca ggtgaacgcg ccaccccgat ggtgctgcac cgtgcgatcg tcggcagcct
     7741 cgaacgcttc atcggcatcc tgctcgagca gcacgccggt gcgctgccgg cctggctggc
     7801 tccggtgcag gtcgtggtca ccccgatcag tgacgggcag gccgattacg cacgcgatgc
     7861 ggcgaaagcg cttcaaaaac aaggagttag agtagtcact gatttgcgga acgagaaaat
     7921 aacctataaa atccgcgagc attcaatgca gaaggttccg tacatcctcg tcgttggtga
     7981 caaggaaaag gcaaacgggg ccgttgcggt gcgcgcccgg ggaaatcaag acctcggtgt
     8041 catgtcgctg gaagacttct cctcgaagtt ggctggcgac atcgctcaaa aagcgtgatc
     8101 tctcctgcca tcggcaggag tgccaagccc tttgaccgac cggtgcgtgc aattcttgtg
     8161 tttggatttc tgtgccttgg aatcccttga aaggttttga ccatcgctac ttttgctgac
     8221 cgccgtaccc ccaatgccga gcgcaagcac cggctgaacc gggagatcat ggcaaccgaa
     8281 gtccggttga acggtgtgga aaacgagccg ctgggcatcg tgagtaccct tgaggcgctg
     8341 cgcatggcag gcgaactgga cgtcgatctt gtcgagatcg cagccaccgc cgagcctccg
     8401 gtctgccgct tgatggacta cggcaagttc aagtaccagg agcagaagcg ggcggccgaa
     8461 gccaagtcga agcagaaggt gatcgaggtc aaggaagtca agttccggcc gggcaccgac
     8521 gagggtgact accagatcaa gatgcgcaac ctgcgccgct tcatcgagga agacggcgac
     8581 aagggcaagg tcacgctgcg ctaccgcggc cgcgagatca cccaccagga catcggcatg
     8641 cgcctgctcg agcgcatccg cgacgaactg gcggatgtct cggtcgtcga gcacatgccc
     8701 aagttggaag gccgccagat gatcatggtg ctagcgccga agaggaagta gaagttcgaa
     8761 gagttcaatg cctcggtctg gtcgccgggg tgttgtcaga agcgactgca ggccaacaag
     8821 acggtggacc tttcaccggc gcctgcagca gcaacaaaga aggagcagtc atgcccaaga
     8881 tgaagaccaa gaagagcgcg gccaagcggt tccgcgtgcg tccggggggc accgtcaagc
     8941 ggggccaggc gttcaagcgc cacatcctca ccaagaagtc cacgaaagtg aagcgccacc
     9001 tgcgtggtgc gactgccgtg cacgagaccg acacggggcg catcaagcag atgatgccct
     9061 tcgccggcct ttaattgact gaaggagaaa tcgcatgcct cgcgtcaaac gtggtgtaac
     9121 agcccgtgcc cgtcacaaga agatcatcgc ccttgccaag ggttaccgcg gccgccgcaa
     9181 gaacgtgttc cgcattgcca agcaagcagt catgcgcgct gggcaatacg cataccgtga
     9241 tcgccgcacc aagaagcgcg tgttccgcaa tctgtggatc gcccgcatca acgccggcag
     9301 ccgcagccat ggcgtcacct acagcaagtt catggccggc ctgaagaagt tgtcgatcga
     9361 gatcgaccgc aaggtgctcg ccgacatggc tgtgcacgat cctgtcgcat tcggcagcat
     9421 cgtcgcgaag gtgaaggccc agctcgcttg accgcctcga cctgtcctcg ggcttcggcc
     9481 tgaggcagcg cggcaccccg gcggcggctt cgttgtcggg ggcaagtccc tcaaggccga
     9541 cctcgtgttc ggcctttttt gttgcggttc cgtttgaacc cagacctcct cccgatgaac
     9601 gacctcgaac ccctggtgca agaagcgcag cgcgactttg cgcaagccgc cacgccggcc
     9661 gatctggaaa atgcgaaggc gcgctatctg ggcaagtccg gtcgcctcac cgatcaactg
     9721 aaggccctcg ctgcactgtc tgccgatgac aagaaggccc gcggcgcgct gatcaatgcc
     9781 gccaagcagc aagtcgaggc ggcgctgaat gcgcgccgcc aggcactggc cgatgccgaa
     9841 ctcgacgccc agctccagtc cgaagcgctg gacgtcacat tgccggggcg ccatcgcggc
     9901 accggcggtc tgcaccccgt gtcgcgcacc atggagcgca tcgaggcgat cttcgggtcg
     9961 atgggtttcg atgtggccga aggccccgag atcgagacgg actggtacag cttcactgcg
    10021 ctgaacaacc ccgagaacca tcctgcgcgt tcgatgcagg acaccttcta cgtcgacatg
    10081 aaggatgctg aaggccgctg gctcaacctg cgtccgcaca cgtccccgat gcaggttcgc
    10141 tacgcgcggg ctcatgcgga gaagcatcgc ggccaggacc cgatgcccga gatccgcgtc
    10201 atcgcgccgg ggcggaccta tcgcgtcgac agcgatgcga cgcattcgcc gatgtttcac
    10261 cagtgcgagg ggctttggat cggcgagaac gtcagcttca aggacctgaa ggttgtcttc
    10321 accgacttct tccgcacgtt cttcgagacc gacgacctgc aactgcgttt ccggccgtcg
    10381 ttctttccgt tcaccgaacc atcggccgag gtcgacatcg cgtttgccag cggaccgctg
    10441 aagggtcgtt ggctcgaggt ggcaggctcg ggccaggtgc atccgaacgt cgtgcgcaac
    10501 tacggcctcg accccgagcg gcacctcggc ttcgccttcg gcatgggccc tgatcgactc
    10561 accatgctgc gctacggtgt gagcgacctg cgtctgttct tcgatggcga tctgcgcttc
    10621 ctttcgcagt tcaagtaaga cctcaccaga agatcccacc cgaaggttca cgcgatgcaa
    10681 tttcccgagt cgtggttgcg cgagttctgc aacccgcccc tgaacaccgc cgaattggcc
    10741 gatctgctga cgatggccgg catggaggtc gaagatctgc gcctggccgc gccgccgttc
    10801 acgggcgtgg tcgtcggcga ggtgctgtct gtcgaacgcc atcctgatgc cgatcgcctc
    10861 aacgtctgtc aggtcgacgt cggtgcagct tcgccgctga acatcgtctg cggagcgccg
    10921 aacgtgcgtg cgggcatcaa ggtgccgtgc gcgatggtcg gcgccgagct gccacccgcg
    10981 gacgacaaga tcagtgtcga acccttccgt atcaagctcg gaaagctgcg cggagtcgag
    11041 agccagggaa tgctgtgctc ggcgcgcgag ctgaagctgt ccgacgaagc cagcggcctg
    11101 ctggtcctgg cgcctgacgc cctggtcggc tgcagcatcc gcgagcatct gctgctcgac
    11161 gacacgctgt tcacgctgaa gctgaccccg aatctcggtc atgtgctgag cgtctacggc
    11221 gtcgcccggg aagtggctgc gctgaccggt tcgccgctgc tgcagccgat gttcccgaag
    11281 gcccgcgtcg agtcgacgca cgtgctgaag gtgagcatcg atgcccctga cctgtgtggg
    11341 cgtttctccg gccgcatcgt gcggcaggtc aatccgaagg ccaccacgcc ggcgtggatg
    11401 gttgatcgcc tggctcgctg cggccagcgt tcggtgagcc cgctggtcga catctccaac
    11461 tacgtgatgt tcgagttcgg ccggccctcg cacatcttcg atctggacaa gatccatggc
    11521 agcttgcagg ttcgctgggg tcgggcgggc gaagccttga agctgctcaa cggcaatacc
    11581 atcgaggtcg acgagaaggt cggcgtgatt gccgatgagc aggccgtcga atcgctggcc
    11641 ggcatcatgg gtggcgacgc gacggccgtg tcagacgaca cgcggaacat ctacgtcgaa
    11701 gcggcgttct ggtggcccga ggcggttgcg ggtcgctcgc gccgctacaa cttctccacc
    11761 gatgccggtc accgattcga gcgtggcgtc gatcctgcga ccaccgtcga gcacatcgaa
    11821 cgcctgacgc aactgatcac cgacatctgc ggcacgcccg ggaccgtttg cggcccgatc
    11881 gacgatcagg tgctttcgct gccggccgcc acgccggtgg ccctgcgcat cgagcgcgca
    11941 gtcaaggtga tcggcatgcc actgacgcag gcgcagtgcg caggcgtgat gcagcggctg
    12001 ggcctggcct gcaccgaaac gcccggggtg ctcacggtga ctccaccgag ctggcgcttc
    12061 gacctgcaga tcgaggaaga cctgatcgag gaagtgatcc gcgtcatcgg cttcaacacc
    12121 ttgcccgaca ccgcgccgcg cgcgcccgtg acggcccgtg tgcggcccga agcgcagcgc
    12181 tcaccgcatg cggtgcgtcg ccagctggct gcgctcgact acttcgagac gatcaatttc
    12241 agcttcgtcg aggaaagatg ggaacgcgag ttgtccggca acgccgaccc gatccgcgtg
    12301 ctgaacccga tcgcgagccc gctgtcggtg atgcgctcca gtctgatcgg tagcctcgtc
    12361 aatacgcttc gctacaacct cgcgcgcaag gcgccgcgcg tgcgcctgtt cgaggtgggt
    12421 cgcgtcttcc gccgtgatgc agctgccgtt gatgcaccgc tgggcgttgc gggcgtggcg
    12481 cagccgatgc gcgtggccgg gctggcctat ggcccggtcg acgccgcgca atggggcaac
    12541 aaggatcgaa cggtcgactt cttcgacgtc aagggcgacg tgcaggcctt gttcgctccg
    12601 cgcatggtgc acttttcgtc gtgcgagcat ccggcgctgc accccggtcg cagtgcacgc
    12661 atcgaggtcg atggggcagt gctcggccac atcggcgaac tgcatccgcg ctggcggcag
    12721 gcctacgagc tgcctggcgc accggttgta ttcgaaatcg acctcgcggc gctcttgcgc
    12781 accacggtgc ctgcgttcgc tccgttgccc aaacagcaat cggtgtggcg cgacatcgcc
    12841 gtcatggcgg acgagtcggt gacgcatgaa gcgctgatgc aatccattct cgaggtaccg
    12901 ggcggattga tccgttcggc caacctgttc gacgtgtttc ggccgccatc ggcaagcgcg
    12961 gcggcctccg agcgcagtct ggcggtgcga ctcgaactgc tggacgatga tgcgaccttc
    13021 accgacgaac gcatcgacgc ggcggtggcc cgggtgcttg agacactgcg cacccggctc
    13081 ggcgtgcgcc tgcggggttg atgatgatga acgacgattc caaacctgtc ccgctgatgc
    13141 tctccagcct ggatacgccc acgctcacca aggccgagct ggccgacctg ctgttcgagc
    13201 gactgggcct caacaagcgt gaatcgaagg atatggtcga agccttcttc gacatcattc
    13261 atgacgcgct catcaccggt cgtgacgtga agatgtcggg cttcggcaac ttcaacatcc
    13321 gccgcaaggc gccgcgaccc gggcgcaacc cacgcaccgg tgaagagatc cccatcaagg
    13381 cgcgcaacgt ggtgactttc catgccagtc acaaactgaa ggatgtcgtg cagggagaca
    13441 cgcctgcgcc ggacgacttc gagtagagtg ttgggtcctc tcctcatgtc gttgatttaa
    13501 atggagaatt cccttcctgc cattcctgcc aaacgctact tcaccatcgg tgaagtgagc
    13561 gatttgtgcg gcgtgaagcc ttacgttttg cgctattggg agcaggagtt cacccagctc
    13621 aagccgatga agcggcgcgg caaccgacgt tattaccagc accacgaggt gctgttgatt
    13681 cgacggatac gcgagttgct ttatgagcag ggattcacca tcagcggcgc ccgaaaccgc
    13741 ttgatcgagc cttcatcggg tgccttgact gcggcagctg cggccgggcc ggaagatgaa
    13801 gagtcgactg tcgcgatgaa cgcgtctggc ggggctgcgg aggcagtgca ggctgttctc
    13861 gatcgcgtcg acctgtctcc cagcgtcgcc tcgacaacca ggccgatggc tatcggcagt
    13921 gcaatgtcga acgcgcagat gcgttcggag ttgttgtcga tccgcacgct tctcacgccc
    13981 tgaacggacc ccgttccaac ctatacttga ggcttgtcgg ggcgtagcgc agcctggtag
    14041 cgcacttgca tggggtgcaa ggggtcgcga gttcgaatcc cgccgtcccg accaatcaaa
    14101 tcaatgggtt agctctcaca aggagctagc ccattttttt tgtaccgcag gcaagtgctt
    14161 gcgaccacac agtgaccggg ttccgctggc tgcgaaccgt ccggaaatcc gtgaaagcaa
    14221 tctgtcttga ggcaggccac catggtgctg tgcttgtccg gcaagcatgc gactgagttt
    14281 ggttgcagcg cgctcaggcg gccagcggcg acctcgagcg ctgcgcatgg gcggcgagcg
    14341 cagcgatgat ctgggtgatg tcgtcatcgc tgtgcgcggc actgaggctg atgcgcacga
    14401 ggcagaggcc ggaaggcgtc gccggcggaa tcatcaggtt ggtgtagatg ccagcgtcga
    14461 tcagtgcttg ccacaaggcc agcgcagcat cgcgcgtttc aaacaccaac gcggcaaccg
    14521 gaccgggtgc cgtggtgccc agccggtagc caaggcgggc ggcctcagcg tagaggcgct
    14581 ccgcgtggct gaaaagtcgt gcgcgcaggt cctgtccctg cagaactcgg cgcagcgcct
    14641 ggtgggttgc tgcaatcacc ggtggtgctg gcgaggccgt gaaaatgtag ggtcggctga
    14701 ccatgcgtat cagttctgtt tcggggtgcc ggcccaggca gaagccgccg atgctgccca
    14761 ggctcttcga gaaggttcca acgatgaaat ccacctcgtc gagcaggccc aattcttcac
    14821 agatgcccag gccgcgcgga ccgaacacgc caaacgaatg tgcttcgtcg accagcagcc
    14881 aggcgccgtg gcgccgcttg atctcgacga tctcgcgcag cggcgcgcgg tcaccgagcg
    14941 tgctgtagag gctttcaacg acgatcaggg tgcggcttgc gtcttcgccc agtcgttcga
    15001 ggcgccgatc caggttttcc gggtcgttgt gcctgaaacg gatcgtcgtg gcgctgctca
    15061 gcctgcagcc atcgtggatg ctggcgtgac tgtcggcgtc cacgagcagg aaatcacctt
    15121 cgctggcaag tgccgagatc gcgcccaggt tggcctggta accggtcgag aagacgatgc
    15181 aggccggcca gccaaaggca ttggccagcg cctgctcgag cgcccggtgg ccggcgtagt
    15241 tgccgctggc catgcgcgaa ccggtggtgc ccgtgcctag ggactcgatg gcctcgaccg
    15301 cggctgcacg gcattccggg tcgtaggtca accccaggta gttgttggtg cctgccagga
    15361 tgacgggacg attgttgatg gtggccgatg tcgccgagtg gacggcgtcc atcggcgtgg
    15421 cgatcggtgg caccgtgctg ctcgcagcca gggtggcctg aagtgctcgc tgggcagcaa
    15481 acttgtcgag gagactcacg tgacgttgcg cgtcaaccgc gcgatgccgg cgcagagttc
    15541 gttgatggtg cgagcctgag ccattgtctc gacgggtatc gagatgtcca ggcggtcctc
    15601 gatctccatg accagatcca tgacgtcggc cgagtcgaga tcggctgcct gagcgagatc
    15661 agcatctccg gggagatctg ccgcctcggg tcggattgtg gcaagtgctt cgcggatcgt
    15721 ggattcgatc gaagcagaag catcgctcat ggacacgctc tcggctgtgg atcctgtcac
    15781 cgaactcacg aggctgcgct ctgagcttcg accctagcgc gcctgctgtc gccgacaaag
    15841 cggcggcggg attcctgtgg ggctgcggcg ctgcgtcgct cgaggaattc cttgcgtgcc
    15901 gtgctccgcg agagcttgcc cgacgaagtg cggggcagcg tgtgcaatgg caccagttcg
    15961 atcaggcaat gaatcccgaa ctccaggtgg atcgcctgct gcagccgctc agccaggccg
    16021 gccagcttgc gcgggtcggc ttcccgacac tgcacgacca gcacggccat ttcaccttcg
    16081 cttgcgtccg gaatcgagaa cgccgatgcg tctgtgtgcc gcacctccgg ctgctgctca
    16141 gccaggtgct caagatcctg cggccagatg ttgcgcccca tgatgatcat caggtccttg
    16201 gcgcggccgg tgatgtgcag cgtgccgtct gcctggtagc cgaggtcacc ggtgtccagc
    16261 caaccgtcgg ccgacagcac ttcgcgtgtg gcctcggcgt tgccgaagta gcccgacatg
    16321 acgctcggac cgcgaagatg gatccgtccg cagcgccgct cgggcagcgg ccggccctgc
    16381 tcatcacgga tctccatttc atagccgggc agcgggggac cgcagtcgat gaatgccttg
    16441 cctttcggca tggccccatc tgccaccaca ggtatggctt cgccatcgtg cgccaatcgg
    16501 tccatgtcga cgacgtcgga gcgagctccg ccttcgagtg gcgcaaagct gaccgccagc
    16561 gagcattccg ccattccgta gcaaggcagg aaggcgcgcc ggtcgaatcc ggccgattca
    16621 agtgaatcgg cgaattcgac gagcgattcg ggatggatca tctcggcacc gatacctgcg
    16681 acgcgccagg ccgacaggtc gagttcttgc gtatccgacg cgcgcagtcg tctggcgcag
    16741 agcgaatatc cgaacggcgg gctgaacgag atcgtcgagc ggttctgcga catcagcttg
    16801 agccacagcc tcgggcgcat ggcgaaatct cgcgtcggca ggtagtcgac tgagcgctgt
    16861 gtggccacgc atcccagaac gatgccaacc agacccatgt cgtggtagta cggcagccac
    16921 gagcagaatc tgtctccggg tgtcaggcgg aagccatggt tgaagatggc tttcagattt
    16981 gccatgaccg cagactgcgt gatcatggtg ccgcgtggga aacgggtgct gcccgaggtg
    17041 tattgcaggt aggcgagttc cgaaggcgtg ggcggcggtg gcggcgtgtc cagggctggc
    17101 agatcggaga attcctccag ggtgcctgct agagccatgg gcagaccctc gctggcttct
    17161 ctcagaaagc cgatgaattc tggcggcgcg aatgctgcaa cggccttgca gtcacgcagc
    17221 aactggcgca ggtggcggat gtaagcctcg cggccgccca gatgcactgc agcgggcaaa
    17281 gggacaggga ccagtcctgc gtactggcag gcgtagaaca tgaccgcgaa atcgacgtgc
    17341 gtgtgcgcga ccagggccat ccgctgaccg cgccccagag gagcgaggtg tcgcgccatt
    17401 tcgagcgcaa gcgagcggat ctcacggtaa gacagcgtgg caatcagctt gccacgccca
    17461 tcgtagtagt tgaagccgga ttcgccttgc gcggcgtaat ccagcgcttc cgccagggtc
    17521 gcgaaatccg cgctgcgcag ttcaatgccg ctagcggtcg gggtggggct gtagagagaa
    17581 ggtgtcgccc cgttcactgg acgcacccct gtcggccgaa ctggcctcgc aagtgagcta
    17641 tcaacccccc cccctttttt attctgcaaa acttcagcaa aacttgaact gcccggaaat
    17701 gtaatttgtg cgccgccgcg ttgcaatgga actgtcacga ttgcagcaac atgtcttggc
    17761 aaacggcgac cggagaagcc acaagtctgt attttgacag gctcgagacc ggttcgcctg
    17821 ccttgacctc aacttgaagt gctgtccagg aagtctgcaa gctggtgaag tgtcgggtag
    17881 tcgaacaggt cggtgacctc gatgcgctgg ggaaactctc gctcgatcgc ttcgtggatg
    17941 cgcgccaggg tcaatgagtt gaggttgatc tcgagcaggt tggtgtgggg cgacagcgtc
    18001 cggtccggga tgaacccagc gcagatcgcc agcaggcgcc cgacggtcgg cgatgtttcg
    18061 tactcccgtg gatccgcagc gctgctcgca gccagcagcg gggccaattc ggtcagaacg
    18121 ctgtcgaatt cccctcgctc gagagcttca gccagcgcat aacgctgcag ctttccactg
    18181 cttgtcttcg ggatctgtcg gacgggcacg acatgcgtgg cttcgaggcc tgactgctgc
    18241 aggatcgcct ttcgcaatgc aagtgccttc aatggaaagt cggcgatgtc gccacgatga
    18301 agcacgaaga tggccagttc ctcggtttcc gctcccgttc ggcgcactcc ggctgcagcc
    18361 acgcgattgg gttcgatccc ctcgacctgt tcggcgatac gctccagatc ctgcgggtag
    18421 tggttcaggc cgttgatgat gatcagatcc ttggcccggc cggtgatcac gagttgtccg
    18481 tcccacagca acccgagatc gccggtgtcc agccatccgt cggccgagcg cacttcggcc
    18541 gttcgcttct cgttccggta atagccctgt gtcacgttgt cgccgcgcat ttgcacatgt
    18601 cccagcatgc gctcttcgag cgttaccccg tcatcgtcga cgattcgaac ttcgttgccc
    18661 ggcagcggtg gaccgacctt cacgaactcg atcagccgcc ctgtgtccgc cgacagggtg
    18721 tccacctgct cccccacgcg cagaattgcc gggctcagct tgacagtttc aagcggtgct
    18781 ccggggcgtg agaagctcac tgcgagggtg gcctcggcca gtccgtaaac agggaaaaga
    18841 gtgttgggct tcagtccatg cggcgccatg gttcgcatga accggcggca caaatcagct
    18901 gaaatgggtt cggcaccatt gaagatcagc cggatagagg agaggtcgag gccttgcggc
    18961 gacttgatgt cgtattgccg caggtagtgc tgaaagccga aattcggtga gcacaacaca
    19021 gtcgatcgtt gctggctcgc cagttccatc cacagcagcg gccgccgcgc gaacagatcc
    19081 gtacgcatga tggtgtggct gataccgccg gccagaaagg tcaggtgaaa acctatcaga
    19141 cccatgtcgt gcgaaagagg catccagctg agaaccaaat cgcggtctgt gtacgcggcg
    19201 ccctcgatga tcgaagcagc gttggccgtg agattgcggt gtgtcagcag gacacccttc
    19261 ggttcgcctg tcgaacctga tgagtactgg atgaaagcga tgtcatccgg ctgggcctgg
    19321 aatggaagcc cgggttctcc ggcaatgtcg agactgccga ccatgacggt gcgcgatctc
    19381 aggagatccc cttctgcctt caggtcgtgc cggcggacaa aggcatcgaa gcgctcaagc
    19441 gcgggcgcgt ccaggcacac cgaaacgcga tcgagctgag caaacacgcg cagcagcttg
    19501 cgccagtgtt cgtcgttgcc gccaggcgcc aacggcaccg gcacgatgcc acccagcaag
    19561 caggcccaga acatttcgac gaagcgctcg ttgtcgccga ggtacaggat cagcatgtcc
    19621 ccgcgggaga acccgcgtcg ctgcaggctg cccagcgcgc caatggctcg ctgccgcaat
    19681 cttctgaacg tcagcgtgcg ctgatcgtgt tctccttcga tgaaggtgat ctgacgatcc
    19741 tctcgcgcgt cggcgggaag aaacgcatct agtgtttcga attgcggcat cgggggttct
    19801 tcagcgcgcc acgacatgca tgtagacgtc ttgacgtgtg cgcaagttga tctggaattc
    19861 ggcctgcggt ggtttgctgg cagcgtatcg aagccggaat cgctgcgcga ccatgctcag
    19921 gtgcatcgtc atttccgtca tcgcgaagct ggcaccgatg cagtagcggg gtcccgcgga
    19981 aaacggcagg aaggcaaagc gatgccgcga ttctgccgcg tcggtcaaaa agcgatccgg
    20041 atcgaaatca tcagggtgct cccagtgcct ggcgtcccgg tgaagcaggt acgggcagat
    20101 gaagatgtcg gtgcctgcgg gcacctgata gcccgcgagc ctgtcatccc tcagtgcgcg
    20161 gcgggtgagc agccagcccg atgggtacag gcgcatggat tcctgcagca cccgttccac
    20221 gtacgagcgg gcttgcggag gctcatcgcc agccgactgc gcaggcgcag gcagcggaat
    20281 tgcgtccgcg atggactcgt gcaggcgaga ttcgacatcg gcgtgctggg acagcaggta
    20341 ccaggtccag ttgagcgtgc tggcggtggt ctcgtggccc gcgacgatca acgtcatgat
    20401 ctcgtcgagg atcgcgcgat cgggcatggc ctctccgctg tccctgtccc gtgcctccat
    20461 cagcatcgac agcaggtcca tctcgatgcg ccgttccttg cggcgcgcat cgatcatggc
    20521 gataacgtgc cgggtcaagg cacggaattt cgctgcaaag ggcaggtcgc gacgtgtctc
    20581 ccgggtcagc aaatcaaacg ggctggatcc ctcggaagcg atcagccggt cgagatcgga
    20641 tccgaacagg gcgcgcagca cgatgccaag ggtcacttca ctgaggtcgc gcgtcaggtt
    20701 gatcggttca cagcgagcgg cgtggccggc ccagcgctcg atcaggtcca ggttgagccg
    20761 ctccatgaat atcgaaaaac cgcgtatcac ctggctgtgg aacatcggct gcatcatcct
    20821 gcgctgcctt gtccatgact cgccgtcgtt cgcgatcagg cctttgccca ggagcacccg
    20881 cacacgttcc aggccgacgc ccttgacgta attgccccgg ttgaccagca gcacatgccg
    20941 gatgtcatcc ggagagtgaa tcaccaggct gtcactggca cggctgcacg ccgtcacacg
    21001 gcatatgtcc ccgaacctcg gcttcaattc ctgcagcagg agcaggcttt cgtcgtcggc
    21061 gtcgaggtcg tggcgttctt gcgggcccgg ggccagcagt tcctgaacca taatcgctat
    21121 tctgttttgg cctgcaaatt gtgtcacgtg cgggcgcgcc aactgccgcc tccatgtccg
    21181 taacggtttt catcacctcc gaattttcgc caacgccggc gatcgaggcc gaatggctgg
    21241 cgcaattgcc gctgcgacgc cggtcggaga ttgccggctg gtctgatcag cgtgcgagac
    21301 ggcaatcgct gctcggcagc cgattgcttt cagccgggct cacgcggctg gggttcgaag
    21361 ctgaaggact ggattcactg agctacggcc ctcggtcccg gccgacactt gatctgccgg
    21421 ttgaattcag cctgtcgcat tgcgaagggc gtgtggtctg tgcgctgtcg acttgcgggc
    21481 ccgtcggcat cgacgtcgag gcgctgggcg atctgacggc cgaggatttc cggatctacc
    21541 tgagtccagc cgagcgatcc tgggctgctc gaagcgcgcg acgcttctac tcggtgtgga
    21601 cccgcaagga ggcggtggcc aaggctgccg gtcatcgggg tctgcgcgac gtggcacggg
    21661 tcgacacggg ggcagcggaa cagcgcgctg ccctggacgg atgcctgtgg cgcacctggc
    21721 cgatcccggc gggccgtcgc cacatggccc acctggcgat ggcggaccag ccgtgcaagg
    21781 tgacggtccg gcgcctcgcc agggacgaga tcgagcacgg tctgcgacca gcggcgcgcg
    21841 tcaggcctgt cgcttcatat caagccgtct tgtaagctcg cgcggttttt ccatccactc
    21901 ttgaaagtcg gcaacgtgtc ccaaccccaa cgcgcggtga ttctcagtgc gggccaaggc
    21961 agccgtctgc tgcctctgac ggcgcaaatg cccaagtgcc ttctcgatct gtgcgggcgc
    22021 agcatgctcg aatggcagct tcggggcctg tcccagatgg gcgtgcccga agcggtggtg
    22081 gtaacgggct ttcacccgga gctggtcgaa gccgaactca agcgcgttgc accgcagaac
    22141 atgcgggtgc gcacgctcta caaccccttc tacaagctgg ccgacaacct cgcgagttgc
    22201 tggatggcgc gcgccgaact gaccgggacc tgcctgatcc tgaacggtga cacgttgttc
    22261 gaacctgata tcgccggccg gctgatagct gcggcatcag cccccatcac cgtcacgatc
    22321 gatcgcaagc cgaactacga ctctgatgac atgaaggtcg agaccgacgg agttcacctg
    22381 cgcgccatcg gcaagacctt gtcggcagag cgcgtcaacg gcgagtcgat cggcttcttg
    22441 cgtttcgagc cggaaggcgc agcgttgttc gtggaggaac tcgaacgcac gatgcgcacg
    22501 cccgaaggcc cgggcaagtg gtacctgtcg gccatcaatc gcctggccga gtctggcgca
    22561 gacatccgcg tggcttcgat cgaaggcctc gagtggggcg agctcgactt cccggatgac
    22621 ctgattcagg tgcgtgccct ggcggccggt tggcttgagc aggaacgggc ttcatcgcgc
    22681 ggcgcttccg ccacggcttg agcgcgttgc cgcgcgtgcg cctgacgcct cagttggcag
    22741 aaccggagca gatgccctcg cgccgaacct cgacgagccg tcgcaccaga tcgaggtctt
    22801 cgggcttgtc gacgtcgatg gcagcgcgtc cgtcgctcat ctgcgcgatg gtgatcctcg
    22861 cgccgccggc cagcgacccc agccgtgctg tcgccgcggt cgaggacagc cagcccagtc
    22921 ggtagcgcat cgcgatcgtc cagcccaggc ggcgcatcat gcgcaacggt gccttgcgct
    22981 cgcgctccag ttcgcgccac aaggtcaccg caccggccgc tgcgggggag gccatcagga
    23041 acaggttgca acccgagaaa tccccgtcgg aaaagcgcag ccaggtgcgc cgcgtgtctg
    23101 gtgcggcggc ctccaccgcg gcgcgctccg ccagacccag aacgacatct gcatcggcgg
    23161 ggcaggctgc caggaactcc tgcacccacg ctgcctccag cagggcgtga tcggcggtcg
    23221 tgaccagcag aggcgtacct tcgcggacca gggcggcagc cacggtggcg ctgggtccgg
    23281 atccggtcgg catcgtgctg accggcttgc agcacaaggg gggctgcagg ccgggcaggc
    23341 cgatcagcag atccggccgc tcgatggcca cgatgattcg tgcgacatcg ggtgcggcgg
    23401 ccagggtgct cacgacccgt tcgatcatcg tggtgccacc gacctcgatc agcgccttgt
    23461 ggctgacgcc ggcataggcg gccaggggat cgcctccggc ccgcgtaccc gccagcacca
    23521 gtgcggtgag ctgtctcaca tcaggagtcc ggcgagcgcg gccgattgca gccggttcgt
    23581 ggtcactgag ccagcgtctt ctcgtagacc cggtacgtct tgtacgggtt ggcgcccagc
    23641 gcttcgatga cgtgccgcat gggccggttg tcctcgagga tccaggagag ttcgacctgc
    23701 ttcacgccct tggcccgcag tcccttgcgc agcgcgtcga tgacgaggaa aggcgccagc
    23761 ccgccagcaa aactggtgct gcagctgcga cggatgccca tcagcggaac gcgcgcggtc
    23821 cgcgcgccac gccgcagccg gtacatcagc ttgatccagt tgaacggaag caggttgcca
    23881 ccgaagtcgg cgatcagttc gttgaggttg ggcagtgcga tgccgaaacc gatggcttcg
    23941 ccgttcatct cgacgatggc cacgcagttc gggtcgatca gcatcttgag gctcttggcc
    24001 atgtgcgcga tctcggcttc ggtgaaggcg atgaatcccc agttctgcga ccaggcgtcg
    24061 ttgaagatcg ccgtgaccgt gtcgaactct tcgcgatagc gctgcatgtc gagccggcgt
    24121 accgtcagcg ccgtggcctt gcgggagtcg atcttgcggc gagctgctgc cggcagctcg
    24181 tgatcgatgt cgtacatgta ggcgatcagg tccttcgcct tcgcgtagcc gagcgcctcg
    24241 attcgcggcc cggcgtaacg cggatcgtgc cccatcagca tcacgggcgg cgcgtcgaaa
    24301 ccgtcgatca acaggccggt ttcctcgttg atcgagaggt tgaaggggcc catgatgcgt
    24361 cgctttccgc gcgcccgcag ccagccctct gcagccgcga acagggccgc gaacacttcg
    24421 gcgtcgtcct cggcgacgat cattccgaag tgaccgacgt cggggtccgt gacgagccgg
    24481 tcgatctggg cgctgatgcg cccgacgtcg cgtccgtcgc gccgtgcgag ccagaactga
    24541 gtctcggcgt gcgcgaagta cggattcttc gtgggtgaaa gggcctcgcg gcgctccatc
    24601 accagcggcg ccacaaaagc cgggtcacct gcgtacaacc tcgcaggcag cttgatgaac
    24661 ctgtccaact ctgccggtga acccaccggg aggatctcga tgccgcccat cacgcagccg
    24721 ggcgcgcctg cgcacgcgat agaaatccat gccagacggt tcgcgcggcg tgccaggcga
    24781 aggtcaggcc ctgccaggcg gtgatgacgt agaaggcggc cgggccgacg cccagcagca
    24841 cgccgaacgc catgatggtc atcgtgatgt tgcgacgggc gatgaagctg cgcacgaatc
    24901 cgtcgagtcg cgaggctgca tgcagcgcga agccgattcg gcgtttcgca atgctcagca
    24961 cgacgcggtc cgcgacatag aagacgatca tccagattgc tgcctggtac agcgggtctc
    25021 cggggttgtg accgccgagg ccccacgcga atgcgaagta ccagaaaggc ggatgcacga
    25081 tgtcggtccc gtggtcgagc agattgccta tcttcgagtc ggtcaaggtc agtcgcgcca
    25141 gcttgccgtc gacgctgtcc agcacgctca tgccgtaggc acagacgaag ccggccagga
    25201 aatcgcctcg tgcgaacaag ggcaccgcgg cgatggtcag cacgatcgac agcagcgtca
    25261 cggtattcgg atggagtccc aggcgcacgc acaggcgcac caggggccag accagcggcg
    25321 gatagacgta gcgagtcagc aggtccgtcg aacccttgta gttgtcccag aacatctgcc
    25381 gctcgagacg ctggcgagtg acggtgtcgg tcacggcatg aatccagtac ggcagcgaac
    25441 gccggagctt gtccatgtaa gccgggaatt ccttctcgtc gaacggcgcg atcagtccgg
    25501 ccgccatcag cgtttcggcc accgtgcgca ggtcctcggc ctgctcgggg atggcatcgg
    25561 ccagttccgg cgccagccgc agcgccactg cccagtgatc gccctcaccc cgtgcggctg
    25621 cacaggaacc atcgcggcgc accagaaacg cgatgagtcg cgggtcgatc
//
LOCUS       DAOORV010000043        25417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2328_length_25417_cov_7.433523, whole genome shotgun sequence.
ACCESSION   DAOORV010000043 DAOORV010000000
VERSION     DAOORV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 25417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_25417_cov_7.433523"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKV56_07810"
     CDS             complement(<1..928)
                     /locus_tag="PKV56_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOX67759.1"
                     /translation="MLALPGLALANADVEKNTANPKNWAMQAGDMFNQRYSKLNQINK
                     TNVGKMQVAWTFSTGVLRGHEGSPLVIDGTMYLHSPFPNKVFAIDLDSQKIKWKYEPK
                     QDPAVIPQMCCDTVNRGLAYAEGKVFLQQADSNLIALDAKTGKLVWSVKNGDPKLGAV
                     NTNAPHVFKDKVITGISGGEWGVRGFLAAYDINTGKQAWKGYSVGPDAEILIDPAKTT
                     TWIDGKVTPVGPDSSLKTWKGDQWKIGGGTTWGWYSYDKATNAVYYGTANPSTWNPSQ
                     RPGDNKWSMSIWSRDVDTGKVNWVYQMTPFDEW"
     gene            1516..2229
                     /locus_tag="PKV56_07815"
     CDS             1516..2229
                     /locus_tag="PKV56_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67760.1"
                     /translation="MLRTTGLTKRYGSRAALQSLTLDLQAGQFVALLGPNGAGKSTLF
                     QVLTGLFAADEGEVEVAGHSLARSASAALRHIGVVFQQIALDLDLSIRQNMLFHADLH
                     GLPRAVALDRIQQGCSRLGIDDDLDRKVRELSGGNRRKVELVRANLHRPSVLLMDEAT
                     VGLDPKSRLDLLAALHADVRERGVCVLWATHWVEEAVGADRVLVLHQGALLADGTPDD
                     VTRVLGEDTLEAGFIARTR"
     gene            2235..3119
                     /locus_tag="PKV56_07820"
     CDS             2235..3119
                     /locus_tag="PKV56_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67761.1"
                     /translation="MSSDPLKPNPGREQSAAQARRGIPHALQALQAIVLREVIKFSQQ
                     TGRLLSALVRPLLWLAVFAAGLHDVFGNTVVPPYDTAVPYDVYVAPGLIGMVLLFNGM
                     QSSLAMVYDREMGLMRLLLTAPLPRAWLLFAKLCATALLSVLQALAFIAIAALIGTSL
                     PVLSLAGLHALFAMICSALMLGALGLLLSVHIKQLENFAGTMNFVIFPMYFMSTALYP
                     LWKLQSSGANWVYELARCNPFTYAVEWMRFALYGKDPGLAPWVVIGSLLVFFGLACWG
                     YDPQRGFGGLTKRGGGSS"
     gene            3116..4153
                     /locus_tag="PKV56_07825"
     CDS             3116..4153
                     /locus_tag="PKV56_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX67762.1"
                     /translation="MTARATTEQAARPRRGADLWWVVAATLSCYGLAIRFELTEFFFG
                     KLAKYERWQIDELPLTFTVLAIGLAWYAFRRQREARIELQRRVQAEARIADLLAHNRE
                     LSRQLINVQESERLALARELHDELGQGCSAIRVETAFIRHCSAAHHADAMRAAARADD
                     AAQGLYQLVRGMLRRLRPANLDSLGLVAALQELCESWEERSGIDCAFHHTGLSDSLGD
                     AIDIAVYRVTQEALTNVMRHARASSVRVMLSHTLGMASEAPGVRLSIQDDGQGMDTAA
                     STRGLGLLGSAERAAGLGGELRVESTPGAGVQISLWIPLAAPAPSAMVTEAAVLAQAA
                     AGGVHHTTAQA"
     gene            4150..4791
                     /locus_tag="PKV56_07830"
     CDS             4150..4791
                     /locus_tag="PKV56_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67763.1"
                     /translation="MIRVMLADDHPVVRSGYQRLLEQAGDIRVVHEAANGEAAYAAFV
                     SDPPDVLVTDLSMPGGGGVTLIRRVVARTASARVLVFSMHDGAQLVRRAFEAGARGYL
                     TKASAPDCLTQAVRTLHAGERYLSPDLPAALLLRDPEQEAERLASLSPREFETFRLLA
                     QGHSVAECAEALKLSAKTISNQQTLIKEKLGMSTSAAMVHLAIRHGVISDSGV"
     gene            4905..5648
                     /locus_tag="PKV56_07835"
     CDS             4905..5648
                     /locus_tag="PKV56_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX67764.1"
                     /translation="MPYPFNTVHALPLTRVALAAGASGASSPSARADISMIRVMLADD
                     HPVVRSGYQRLLEQAGDIRVVHEAANGEAAYAAFVSAPPDVLVTDLSMPGGDGMTLIR
                     RVMARSASARVLVFSMHDGAQLVQRSFEAGARGYLTKASAPDCLTQAVRALHAGERYL
                     SPDLPATLLRDPEQESERLASLSPREFETFRLLAQGHSVAECAEALRRSAKTISNQQT
                     VIREKLAVSTSAAMVHLAIRHGVISDSGV"
     gene            5789..8113
                     /locus_tag="PKV56_07840"
     CDS             5789..8113
                     /locus_tag="PKV56_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX67765.1"
                     /translation="MPHSINKVRLQPLAWAALAACATAAHSPSALAQAAPTAASSAEL
                     PRVEIIRVTPLPGLGIAKDQVPSNVQTLNDRKLKQMQSLNLPDALATQMPSVTVNEIQ
                     GNPYQLDVNYRGFTASPLLGTPQGLSVFLDGVRINEPFGDTVNWDLIPKAALSNITLL
                     PGSNPLFGLNTLGGALSLQTKSGDTHPGTEIEAQAGSFGRYSVDLAHGQTFGETSHAF
                     LAASFFDEDGWRDFSPSRVKQLFGKFGQRDKDYSWDLSLNAADNRMIGNGLLPEALLK
                     QNREGIYTRPDLTRNKMTMLSLNATARIDAEQQLAFTAYSRRTRTSTLNGDLNDDFEF
                     GVDDEDEAGVENRTRTRQRGEGLALQWTLARDIHRLTLGASHDRSRTRFAQTEAVGAL
                     SDSRGVIPLEEQELDALISGRTRTSSAYFTDLIELRPNLQLTLSGRYNDTRVRTIDRG
                     RLLDEPTDTELDGKGRYKKFNPAAGVTWQVSPVLTAYGSYSQGNRAPSPIELGCSDPD
                     NACVLPNALQSDPPLKQVVSRTIEAGLRGSLPGDVRWNASAFRTVNKNDLLFISTGAA
                     TGYFSNFGRTLRKGIEMSLSQQVGELNWSLAYSYLRATYDSPACIVAEANSTAETSSR
                     CTGDDEIEVRPGDRIPGQPKHNLKVNADWRVLPQWTVGAQVSAYASQYVRGNENNKHQ
                     PDGNDFNGKGKIGGYALVNLTTSLNLGSGWELFAKVTNVFDREVATAGALGENSFTGN
                     GSELQAAVSDQFVGPNAPRAGWIGLRLRFAGIGE"
     gene            8233..9255
                     /locus_tag="PKV56_07845"
     CDS             8233..9255
                     /locus_tag="PKV56_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX67766.1"
                     /translation="MDRIDALLADGRQRALALEVEVGKAVIGQSRVIRLINTAVFARG
                     HVLLQGDVGVGKTTLLRAFAQVLGGDFARTEGTIDLMPNDLIYYTYISADGRPAVEHG
                     PLIKHGEDLAIFFFNEINRARPQVHSLLLRVMAERSVSAFNREFRFPHLLVFADRNRV
                     EREETFEISSAARDRFMMEITIDAPATDADRRRLIFDPRFHDTDRLVGSVQAAMVAYR
                     ELNELAATIQATVQASETLETYAMHLWRATAVPGEYGIHISDIDIDQLMLAGASPRGM
                     SMLLRASRVAAWLEGRSYVTPEDVQAVFVETIAHRLCYQPVFELRRQEISGSLMAGIL
                     GSVAAP"
     gene            9279..10115
                     /locus_tag="PKV56_07850"
     CDS             9279..10115
                     /locus_tag="PKV56_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX67767.1"
                     /translation="MPEIHYRIGAPARGYFPGHHRSKSGESGFEFRGHASLLDAPDPR
                     RLDLHASLRDPFGNWIVRVYSQRKSIPVAVVADLSASMGFEGARRKLDVLADFTDSLA
                     WSVWRTGDSFGFVGCDEQVRADLLLPQTRVRGVGQALAGRLRGLSIEGRSAHGLVDAH
                     RHLSRQRSLVFLLSDYHLPLAMLRQVLRDLAQHDVVPVVVWDPAEFTLSAPRGLACGI
                     DPESGERRLIWWRPALREKWASALAARRHDLQALFRAMRLKPLFIEQGFDADAVTRHF
                     HS"
     gene            10112..11095
                     /locus_tag="PKV56_07855"
     CDS             10112..11095
                     /locus_tag="PKV56_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67768.1"
                     /translation="MSALPGHRQRSRRVIAALALAVAVLATPRVGAASVDDPGPAAWQ
                     IDTVEPRPYGHTIGDVVQRRVVIHVPAGHTLDEETLPSAGHRSGSIELRRLARELDRD
                     GSGSRLTLTMTYQVFLAPREVRTLELPPFVLGFKGAQQARDVRVEAWPLTVSPLVPLD
                     VSPRRGLGELQPDAQPPLIDTRAGRWRLAGYGVIAALLLAYLALVYVGLPWFSRRQRP
                     FTQACRALKAMAGQSRADPASPEQRRQAFQHLHDALNQTAGEVVFEQNIERFIAAQPR
                     FAGLREDLVLFFRHSRAEFFAGAGAAPDRALPAVWLINLCRKCRDAERGAA"
     gene            11092..12138
                     /locus_tag="PKV56_07860"
     CDS             11092..12138
                     /locus_tag="PKV56_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOX67769.1"
                     /translation="MNFDTPWLLALLPLALLPWWARPRHALSNGWLAMMPRDRASDAI
                     SFALRAASVGALAALIVGAAGPYRPEYTVQKVGKGAEIVLVLDRSRSMDQGFAGGAGG
                     PPPGTRPNSPEALEYYTSRNAAASRDSKGKVARQLLSEFAAQRPEDRFGMVLFSTLPI
                     RTLEFTQKPEAIQAAITAGNIGRGLADTDIGLALSSALSYFDDRPYTGSRILMLVSDG
                     GDRIDVDVRERVAYLARKHRVAIYWFYIRSSLSPGLMADASEPPSNVDTVPEYFLHRF
                     FASLGTPYKAYEAENPQALQKAIDDVNRLENLPITYLDTVPRRDLSQLGYGIALACVL
                     ALLGANLMEIRRWA"
     gene            12129..12701
                     /locus_tag="PKV56_07865"
     CDS             12129..12701
                     /locus_tag="PKV56_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaK protein"
                     /protein_id="HOX67770.1"
                     /translation="MGLSSRIAHWPSRRRTRAVILVLALLALVAAVDGARIARQHGIN
                     DAIRSGQLPAEAPGQPREIQFARAAAAAPGPGGSEEDVLNRYRALQDDTALGRAARFN
                     TANALMRQAIALRSTAQPGQAIPLIELAKETYRELLRADPANWGARYNLERAQRLLPD
                     PEDSDAPPSEPPSNAERAATTMRGYSPGLP"
     gene            12718..13782
                     /locus_tag="PKV56_07870"
     CDS             12718..13782
                     /locus_tag="PKV56_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOX67771.1"
                     /translation="MSWLRRGLAACRVSIRGAFTRHLDRTLLIVASLALAATFLNPVA
                     TVDRALFDQVIVLDITQSMNVTDYQLEGKPVSRLSYAKHALRQLLLELPCGSKVGWGV
                     FTEYRSFLLIAPIEVCENLNELRSTLDNIDGRMAWTGNSEVAKGLFSGIGIARQLQDK
                     PSLVFVTDGHESPPVNPQHRPVFNDKPGVVQGLIVGVGGLRPFPIPKLDPLGRPMGLW
                     GADEVMQTDPRSQGRGGSVGHEQMVEDPADAGKAANLPGSTPGSEHLSAMREGYLRLL
                     ADDTGLSFLPLQQPEALMRALTAPQLAQSVPAATDLRVALAALAFVLLLARSAMAVWQ
                     RFETRQRSPRLGRKPSVRGQ"
     gene            13848..14141
                     /locus_tag="PKV56_07875"
     CDS             13848..14141
                     /locus_tag="PKV56_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67772.1"
                     /translation="MATQRTKTPASSTAAAAAASADSVKLRQTQAEYSAARPSSEPGI
                     KPMMSSSKMYVCRRCQANFKTGEGKPDPRLRGLGRCNKCLDAATASQSAALAA"
     gene            14442..14876
                     /locus_tag="PKV56_07880"
     CDS             14442..14876
                     /locus_tag="PKV56_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOX67773.1"
                     /translation="MDSLSAIAYVSSALGDVSEARLEALLVDSRGFNLSKGVTGVLLY
                     HDGKFFQYIEGPVSGVEEVYARIKASRRHFGLVELLHERIHGRVFSEWSMGFACAPRN
                     FILQLERQRWSDVSAQLTSGAEVSAGLDFLLQFLKGSTRGMS"
     gene            complement(14951..15724)
                     /locus_tag="PKV56_07885"
     CDS             complement(14951..15724)
                     /locus_tag="PKV56_07885"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOX67774.1"
                     /translation="MKTVPSLFVSHGAPTFALEPGVAGAALSALGRRLPPLSAVLIVS
                     PHWITTGFRVTSGTRPATIHDFSGFPPALYELQYPAPGDPARAAEIVRLLASAGIDVT
                     LDAQRGLDHGAWVPLRHLLPTASTPVLQLSLSDTLDARGALRLGEALRPLREQGVLVV
                     GSGSLTHNLGEFRGPATEAGYVADFVQWIRQAVAERDTAALVDYRHRAPHAVRSHPTE
                     EHFLPLLVAVGATSDADRVDVIEGGTLYGMLSMESYVFE"
     gene            15805..16659
                     /locus_tag="PKV56_07890"
     CDS             15805..16659
                     /locus_tag="PKV56_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOX67775.1"
                     /translation="MSVHLTSAPDLCTARPRLWGELCTTDFATLDPDTTVAVLPVSAT
                     EQHGPHLPLSTDAVINSGVLHAALARLPAAAATSILQLPPLAVGDSLEHSAFPGTVSV
                     DSDALMGLWLSIGRSVARAGVRKLVIFNSHGGQKAHVDLVAVRLRVECRMLVVRAHSF
                     GFGVPPGLFDVEERAHGIHAGAVETSLMLHLRPDLVRQSALKTFPSLGQQLVARGGTL
                     GVEKPVGFGWMTQDLNPEGACGDATQASAAKGAAVLDHMAEGLARLLQDVIALPLDTL
                     RDGPLPTG"
     gene            complement(16697..17890)
                     /gene="ribA"
                     /locus_tag="PKV56_07895"
     CDS             complement(16697..17890)
                     /gene="ribA"
                     /locus_tag="PKV56_07895"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOX67776.1"
                     /translation="MNDRYQEHRTPTPASAAEEQAATRIDRAQTELRHGRAIRLIDRD
                     KPGEASADSCVVAAVEALTEPRLRWMSSLGASVHLLLTIERALALGIEADSTGSLAAT
                     SLRLRLPDDISLDALLGLAAVTSSGLSAVAALPEWAASERTPPVRAVAMDSALQLARR
                     ARLIPALLVVDVPAALEPALAAEQLLWVSSADLSKVPAGGGASLRRVSDAQVPLAAHD
                     DCELVLFREDHGGAEHVAIVVGQPDFSQPVPVRLHSSCLTGDLLGSLRCDCGDQLRRA
                     IERLAETGGVLLYLEQEGRSIGLANKLRAYRLQDDGLDTIDADRYLGFAADDRDYGAA
                     VAMLKSLGIARIRLMTNNPRKIAAMEAGGIEVVERLALHGPVNPFNERYIETKQQRAG
                     HLKPE"
     gene            18225..18710
                     /locus_tag="PKV56_07900"
     CDS             18225..18710
                     /locus_tag="PKV56_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOX67777.1"
                     /translation="MTIKQGDRVPGGSFKQLTTTGVVDISTDSLLKGKKVALFGLPGA
                     YTPVCSASHLPGYVDNAEKLRKAGFDTIACVSVNDPFVMDAWGKSTGADDKVLMLADA
                     DGSFTRAIGLVIDLPAFGLAGRSERYSMIVEDGVVTRLEVEKSVLDHDGSSAACMLRN
                     S"
     gene            19151..19375
                     /locus_tag="PKV56_07905"
     CDS             19151..19375
                     /locus_tag="PKV56_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67778.1"
                     /translation="MTFITSADAKELFWSEIGVADKFKPQHTKFLKYLEGHDSVTYED
                     AMKQLDSLIGELTPNDNPPRRMLMSQPGAR"
     gene            complement(19501..20373)
                     /gene="bioD"
                     /locus_tag="PKV56_07910"
     CDS             complement(19501..20373)
                     /gene="bioD"
                     /locus_tag="PKV56_07910"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOX67779.1"
                     /translation="MMTAGGDPSNGWQATEAGVLWEPEARAADLAEPDTDRPLRGCFV
                     TGTDTEVGKTTVSAGLLHALGRQGLRVAGYKPVSAGAVWCSAASGPGGHWHNEDVEAL
                     LAASNIELTRSEVCPCLLEEACAPHLAARLEGGHIEPRVLTAGARRLMPRCDVLVVEG
                     VGGFCVPLVEAEEVEDGADAGGWGADDLASALALPVILVVGLRLGCINHSLLTAAAIN
                     ARGLRLAGWIANRVEPDMAHAQDNIDTLRRALQRRYGAPLLGVIDYMVTATPDRVANQ
                     LDVGAVIEVLGLPD"
     gene            complement(20370..21605)
                     /gene="bioF"
                     /locus_tag="PKV56_07915"
     CDS             complement(20370..21605)
                     /gene="bioF"
                     /locus_tag="PKV56_07915"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOX67780.1"
                     /translation="MLKHLEQQLAEREAQSLRRQRRIAESACAPHQLVSSNDCAPREL
                     LTFCSNDYLGLANHPALVDALAEGARLYGAGSGASHLISGHSRAHALLEDDLAEMMSP
                     HLPEVRALYFCTGYMANMAVLTALGDAQATIYTDLLNHASLIDGARLAKAAVHTYPHN
                     DLGGLERRLAACSTPRKLIVTDGVFSMDGDLARLPELLALAERHDAYIVVDDAHGFGV
                     LGEEGRGVLSHFGLRSQRLICMGTLGKAAGAAGAFVAAHRVVIEWLVQSARAYIYTTA
                     SPPAVAHAVRASLRLIRGPEGEQRRLHLRELIAALRQGVPEVLGDVLLERGWHLAESQ
                     TPIQPLIVGGSAEALAVAAGLESRGLWVPAIRPPTVPSGSARLRITLSAAHRHDDVKR
                     LLSGLAAAARAAGEPGGAT"
     gene            complement(21636..23018)
                     /gene="bioA"
                     /locus_tag="PKV56_07920"
     CDS             complement(21636..23018)
                     /gene="bioA"
                     /locus_tag="PKV56_07920"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOX67781.1"
                     /translation="MSTPTDRSASSTHADWQRRSVASVWHPCTQMQRMDAVPPIAIAR
                     AQGPWLEDVDGKRYFDATSSWWVNLFGHADERINAALKHQLDTLEHVMLAGCTHAPAV
                     ELAERLSARTGQALGHTFFASDGASAVEIALKMSFHSWKNRGQGDRREFVCLRHGYHG
                     ETIGALAVTDVAVFRDAYDPLLMRSHVVMAPDARQAEPGESAADVAHRAAAELATLFE
                     ARAGHIAAVILEPLVQCAAGMAMHDPAYLREVRALCDRYQVHLIADEIAVGCGRTGTF
                     FAFEQAAAVGEPAIWPDLLCLSKGISGGYLPLSLVLSTDAIYTSFLDDDTARGFLHSH
                     SYTGNPLACRAALAVLDRFDEEPVLQRNRDTGALLTAALAARLAGDARIEHVRQRGMI
                     WAFDVREAHAGVRFAERFHLIARTQGLLIRPIGRTVYLLPPYVLNEEHVAYLADRTVA
                     TLNATLSAGH"
     gene            23188..>25417
                     /locus_tag="PKV56_07925"
     CDS             23188..>25417
                     /locus_tag="PKV56_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOX67782.1"
                     /translation="MDKHYLTPLFAPSSIVVLTGGAAPQTAAARALHAALKAQAFAGT
                     LTFLDVNATGTLSDLAHARADLAVIAMPPDEVLTALAAAGRIHCRAALVISSNVDAAT
                     VAEMHHQARLDGMLMLGPNGLGFQRPGLQLNASIAGPLPASGSLALVSQSGALTSAIL
                     DWGNSNGVGFSTVVSLGPHPSVDLAQVLDFLASDAQTHSIVVYLEGITHARRFMSALR
                     AAANAKPVVVLKAGRQPAGNAAALTHSGAIVGNDDAFDAALRRAGAVRVRSFVQLFSA
                     AKCLASRYRAVGPRLAIVTNGGGPGVMAADWVSEIGLQLGRLSPAEADTLRPRLAPGA
                     TMSDLIDLAEDATAEQFEAAIAAATQSTQIDGVLAIYSPKTGTDSAAIARALVTVKKR
                     SEKPLLSCWMGDASVVEGRRLLSDAGIPTFRTPESAVGAFGNIAAYHRNQQLLQQTPP
                     PLSDLAKPDVEGARMLIESVLTERRQVLTEMESKALLAAFHIPVTKTLLARSVHEAVL
                     IATQLGYPVALKIDSPDISHKSDVQGVVLNVVNAIGVRDTYNEMLRSVTRQLPEARIN
                     GVTIQKMSGQQRGREVCIGLVTDEPFGPVITFGAGGTMVELIDDHAMELPPLNQFLAR
                     RLIERSRVAETLGAWRGAAPADMESLEQVLLRVSEMVCELPQLREMDINPIIVDESGA
                     LAVDARIVIAQRPESTAASNHHQYHHLAILPYPSNHAQLWPLPGGGEYTVRPIQPDDA
                     TML"
BASE COUNT         4292 a         8616 c         8278 g         4231 t
ORIGIN      
        1 cccactcgtc gaacggcgtc atctggtaga cccagttgac cttgccggtg tcgacatcac
       61 gagaccagat gctcatcgac cacttgttgt cgccagggcg ctgcgaagga ttccacgtcg
      121 acggattggc cgtgccgtag tagaccgcgt tggtggcctt gtcatagctg taccagcccc
      181 aggtcgtgcc gccaccgatc ttccactggt cgcccttcca tgtcttcaac gacgagtcag
      241 ggccgaccgg agtcaccttg ccgtcaatcc aggtggtggt cttggcggga tcgatcagga
      301 tttcggcgtc gggtccaacg ctgtagccct tccaagcctg cttgccggta ttgatgtcat
      361 acgcagccag gaatccgcga acgccccact cgccgccgga gatgccggtg atgaccttgt
      421 ccttgaagac atgaggcgcg ttggtgttga cggcacccag cttcgggtcc ccgttcttca
      481 ccgaccagac cagcttgccg gtcttggcat ccagggcgat caggttgctg tcggcctgct
      541 gcaggaaaac cttgccttcg gcataagcca gaccgcggtt caccgtgtcg cagcacatct
      601 gcggaatcac ggccggatct tgcttcggtt cgtacttcca cttgatcttc tgcgaatcga
      661 ggtcgatcgc gaacaccttg ttcgggaacg gcgagtgcag gtacatcgtc ccgtcgatca
      721 ccagcggaga cccttcgtga ccacgcagca cgccggtgga gaaggtccag gcaacctgca
      781 tcttgccgac gttggtcttg ttgatctggt tcagcttgct gtagcgctgg ttgaacatgt
      841 caccagcttg catcgcccag ttcttggggt ttgcagtgtt cttctcgaca tctgcgttgg
      901 cgagtgccaa acctggcaac gcgagcataa ctacgctcaa accacacacc gcagcgcgtg
      961 cattcgattg cccggaaatt cccattgctt tctcctgttg tcaaaggtgg tttaggcaag
     1021 gtcgcggggc accaccaaaa acccaccgcg actctcagag catgttgcca agatggcaac
     1081 ctttgtgccc gcagtcgctt gtgatgttca agtttgaacg aacacattcg cgatcaacca
     1141 tggtcaggaa attttcccct gaggcgtgcg atggaaaacc cgtaattcat gcctggcgga
     1201 tgttgttttt ttcgttgccg aagcgattcg agggaacgtc cgcataggga aactccgcag
     1261 atcagcgcgc ggtcgagctg cagagccagt tgcagctgag ttcgggaatt gccgggccgc
     1321 gcgatccccg ccagctgaag acgacacgca ctgtgcttga tggcggaaca gaccgttcag
     1381 gaattgacac agtcctcgac acgggatcgg ctgcccgcgt gctgaagatc gggcgcaaca
     1441 cccgcgtgtg agcggcacag ggcatagggg agcagcggcg cgcaacgaga tttgtccact
     1501 acattccacg ctcccatgtt gcgcaccacc ggactcacca aacgctatgg ctcgcgtgcc
     1561 gccctgcagt cgttgacgct ggatttgcag gccggccaat tcgtggcttt gctcggcccg
     1621 aacggagccg gcaaatcgac gctgttccag gtcctgaccg ggctgttcgc cgccgatgag
     1681 ggcgaagttg aagtggccgg gcattcgctc gcacgatcgg cctctgctgc gctgcgtcac
     1741 atcggggtgg tattccagca gatcgcgctc gatctcgacc tgagcattcg gcaaaatatg
     1801 ctgtttcatg ccgatttgca tggccttcca cgcgcagtgg cgcttgatcg catccagcaa
     1861 ggctgcagtc ggctcgggat cgacgacgac ctggatcgaa aggtgcgcga gttgtcgggc
     1921 ggcaaccggc gcaaggtgga actggtgcgc gcgaacctgc accggccttc ggtactgctg
     1981 atggacgagg ccactgtcgg tctcgacccg aaatcgcgcc tggacctgct cgccgcgctg
     2041 catgctgacg tgcgagagcg tggcgtctgt gtgctttggg caacccactg ggtcgaagag
     2101 gcggttggcg ccgatcgcgt gctggtactg caccagggcg cgctgttggc cgacggcaca
     2161 cctgacgacg tcacccgtgt gctgggcgag gacacgctcg aagccggctt catcgcccgc
     2221 acccgttgat ctgcatgtca agcgacccat tgaagcccaa cccaggtcgc gagcaatcag
     2281 cggcccaggc aaggcgcggg atcccccatg ccctgcaagc gctgcaagcg atcgtgctgc
     2341 gcgaggtcat caagttctcg cagcagacag ggcgactgtt gtcggcactg gtccgtccgc
     2401 tgctttggct ggcggttttc gctgccgggc tccacgacgt ctttggcaac accgtcgtcc
     2461 cgccgtacga caccgctgtg ccctacgacg tgtacgttgc tcccgggctg atcggcatgg
     2521 tgctgctgtt caacggcatg cagtcgtcgt tggcgatggt ctacgaccgc gagatgggcc
     2581 tgatgcgcct gctgctcacc gcaccgctgc cgcgcgcctg gctgctgttc gccaagctgt
     2641 gcgccacggc cttgctgtcc gtgctgcagg cactggcgtt catcgcgatt gccgccctga
     2701 tcggcaccag cctgccagtg ctttcgcttg cgggtctgca tgcgctgttc gcgatgatct
     2761 gcagcgccct gatgctcggc gcactggggc tgctgttgtc ggtgcacatc aagcagctcg
     2821 agaactttgc tggaacaatg aacttcgtca tctttccgat gtacttcatg tcaaccgcgc
     2881 tctacccgct gtggaaactg cagtcatccg gcgcgaactg ggtgtacgag ctcgctcgct
     2941 gcaacccatt cacctacgcg gtcgagtgga tgcgatttgc cctctacggg aaagacccgg
     3001 ggctggctcc gtgggtggtg atcggcagcc tgctggtttt ctttggcttg gcttgctggg
     3061 gctacgaccc tcaacgcggt ttcggcgggc tgaccaagcg gggcggagga agttcttgac
     3121 tgcgagagcc acgaccgaac aggccgcacg gccgcgacgt ggcgccgatc tctggtgggt
     3181 cgtcgccgcg accctctcgt gctacggatt ggccattcgc ttcgagctga ccgagttctt
     3241 cttcggaaag ctggccaagt acgagcgttg gcagatagac gaactcccgc tgaccttcac
     3301 cgtgctggcg atcgggttgg cctggtacgc attcaggcgg cagcgagaag ctcgcatcga
     3361 gctgcagcga cgggtgcagg ccgaggcgcg cattgccgac ctgctcgcgc acaaccgtga
     3421 gctgtcgcgc caactgatca acgtccagga aagcgagcgg ctggcgcttg cgcgtgaact
     3481 gcatgacgag ttgggacagg gctgcagcgc gatacgcgtc gagacagcgt tcatccgcca
     3541 ctgctcggct gcccatcatg cagacgcgat gcgcgccgcc gcgcgtgccg acgacgcggc
     3601 gcaagggctg tatcaactgg tgcgcggcat gttgcggcgc ctgcgtccgg ccaacctcga
     3661 cagcctcggc cttgtcgcgg cacttcagga gctctgcgaa tcatgggaag agcgcagcgg
     3721 cattgattgc gcgttccacc acaccggtct gtccgattcg ctgggtgatg cgatcgacat
     3781 cgccgtctac cgcgtgactc aggaagcact gaccaacgtg atgcggcatg cccgtgccag
     3841 cagcgtgcgg gtgatgctgt cgcacacgtt gggcatggcc agcgaggcgc cgggcgtgcg
     3901 gctgagcatc caggacgacg ggcagggcat ggacacggct gcgtcgactc gcggcctcgg
     3961 gctgttgggt tcggcagagc gcgctgcagg cctcggtggc gaactccgcg tggaaagcac
     4021 accgggcgcc ggcgtgcaga tttcgctgtg gattcccctg gctgcgcctg cgccgagcgc
     4081 catggtcaca gaagcagccg tgctggcgca ggccgctgcc ggcggggtac atcacacgac
     4141 tgcacaagca tgatccgcgt catgctcgcc gacgaccacc cggtggtgcg cagcggctac
     4201 cagcgcctgc tcgagcaggc gggcgacatc cgggtcgtgc acgaagccgc gaatggcgag
     4261 gccgcctacg ccgcattcgt cagcgacccg cccgacgtgc tggtcaccga cctgtcgatg
     4321 cccggcggtg gcggcgtgac gctgatccgc agagtcgtgg cgcgcactgc ttcggccagg
     4381 gtgctggtgt tcagcatgca cgacggtgcg cagctcgtgc ggcgcgcctt cgaggcaggt
     4441 gcgcgcggct acctgaccaa ggccagtgcg cccgactgcc tgacgcaggc ggtgcgcaca
     4501 cttcatgcgg gcgagcgcta cctgagcccg gacctgcccg cggcgctgtt gctgcgcgat
     4561 cccgagcagg aagccgagcg gctcgcgtcg ctgagtccgc gcgagttcga gaccttccgc
     4621 ctgctggccc agggccactc ggtcgccgaa tgcgccgagg ccctcaagct gagcgccaag
     4681 acgatttcca accagcagac gctgatcaag gaaaagctgg gcatgtccac ctcggcggcg
     4741 atggtgcacc tggcgatacg ccatggcgtg atcagcgact cgggcgtgta gcccgagcgg
     4801 cgggagattc cggccgcgtg acgggaaatt tcccctgtct tcggggaggt gatgtcggta
     4861 agttgtcgca ccggttttca cctcacttcc cgaagacgat cactgtgcct taccccttca
     4921 acacggtcca tgcgcttcca ctgacccggg tcgcgctggc cgccggtgca agcggcgcca
     4981 gcagtccgtc ggcgcgtgcc gatatcagca tgatccgcgt catgctcgcc gacgatcacc
     5041 cggtggtgcg cagcggctac cagcgcctgc tcgagcaggc gggcgacatc cgggtcgtgc
     5101 acgaagccgc gaatggcgag gccgcctacg ccgcgttcgt cagcgccccg cccgatgtgc
     5161 tggtcaccga cctgtcgatg ccgggtggcg acggcatgac gctgatacgc cgtgtcatgg
     5221 cgcgtagtgc ctcggccagg gtgctggtgt tcagcatgca cgacggtgcg cagctcgtgc
     5281 agcgatcttt cgaggcaggt gcgcgcggct acctgaccaa ggccagtgcc cccgactgcc
     5341 tgacccaggc agtgcgcgca cttcatgcgg gcgagcgcta cctgagcccg gacctgcccg
     5401 caacgctgct gcgcgatccc gagcaggaat ccgagcggct cgcgtcgttg agtccgcgcg
     5461 aattcgagac cttccgcctg ctggcacagg gccactcggt cgccgaatgc gccgaggccc
     5521 tcaggcgcag cgccaagacg atctccaacc agcagacagt gatcagggaa aaactggcgg
     5581 tgtcgacctc ggcggcgatg gtgcacctgg cgatacgcca tggcgtgatc agcgactcgg
     5641 gcgtgtagcg gaatcggggc atacccgctg cggcccgtct ggtgaagacc ctcgaccccg
     5701 ccacaaaggg aaattttccc tgtattcgat ctgccggcaa cggtaacttg ccggaccctt
     5761 gttcgtcatc acatctccag gatccctcgt gccccattca atcaacaagg ttcgtctgca
     5821 accgctggcc tgggccgctc tggccgcctg cgcaaccgct gcccacagcc catccgccct
     5881 ggcgcaggca gccccgaccg ccgcttcgtc agccgagttg ccgcgcgtcg agatcatccg
     5941 cgtcacaccg ctgccagggc tggggatcgc caaggaccag gtgccatcga acgtccagac
     6001 gctcaacgac cgaaagctca agcagatgca gagcttgaac ctgccggacg cgctggccac
     6061 gcagatgccg agcgtcacag tcaacgagat ccagggcaac ccctatcagc tcgacgtcaa
     6121 ctaccggggc ttcaccgcca gcccgctgct cggcacgccg caaggcttgt cggtgttcct
     6181 ggatggcgtg cgcatcaacg agccgtttgg cgacaccgtc aactgggacc tgataccgaa
     6241 agcggcgctg tccaacatca ctctgctgcc gggctcgaat ccgctgttcg ggctcaacac
     6301 cctgggcggg gcactgtcgc tgcagaccaa gagcggagac acccatccgg gcaccgagat
     6361 cgaggcacag gccgggtcct tcggacgcta cagcgtcgac ctggcgcatg gccagacgtt
     6421 cggcgaaacc agccacgcgt tccttgctgc cagcttcttc gatgaagacg gctggcgcga
     6481 cttctcgccg tcacgcgtca agcagttgtt cggcaagttc ggccagcgcg acaaggatta
     6541 ctcgtgggac ctgagcctca acgcagcaga caaccgcatg atcggcaacg ggttgctgcc
     6601 cgaggcactg ctcaagcaga accgcgaggg catctacacg cgccccgacc tgacgcgcaa
     6661 caagatgacg atgctcagcc tcaatgccac cgcacgcatc gacgctgaac agcaactggc
     6721 attcaccgcc tacagccgcc gcacgcggac gtcgaccctg aatggcgacc tgaacgatga
     6781 tttcgagttc ggggtcgacg atgaagacga ggccggcgtc gaaaaccgca cccgcacccg
     6841 ccagcgcggc gaaggcctgg cgctgcaatg gacgctggcg cgcgacattc accggctgac
     6901 gctcggcgcc tcccacgatc gttcacgcac ccggttcgcg cagaccgagg ccgtgggcgc
     6961 gctcagcgac tcgcgcgggg tcattccgct cgaagagcag gaactcgatg cgctgatttc
     7021 cgggcgcacc cgaacgtcca gcgcctactt caccgacctg atcgaactgc gtccgaacct
     7081 gcagctcacg ctgtcgggcc gctacaacga cacccgcgtg cgcaccatcg accggggtcg
     7141 tctcctcgac gagccgaccg acaccgagct ggacggcaag ggacgctaca agaaattcaa
     7201 tcccgcggcc ggcgtgacgt ggcaggtatc gccggtgctg accgcctacg gcagctacag
     7261 ccagggcaac cgggctccga gcccgatcga gctcggatgt tcggatccgg acaacgcctg
     7321 cgtgctgccg aacgcactgc agtccgaccc gccgctcaag caggtcgtgt cgcggaccat
     7381 cgaagccggg cttcgcggca gcctgccagg agacgtgcgc tggaacgcat cggcgttccg
     7441 caccgtgaac aagaacgacc tgcttttcat cagtaccggg gcggcgaccg gctacttctc
     7501 gaacttcggc cgcaccttgc gcaagggtat cgagatgagc ctgtcccagc aggtgggtga
     7561 gctcaactgg tcgttggcct acagctacct gcgcgcgacc tacgactcgc cggcctgcat
     7621 cgtcgccgaa gccaacagca cggcagagac cagttcgcgt tgcacgggtg acgacgagat
     7681 cgaggtgcgc cccggtgatc gcatcccggg ccagcccaag cacaacctca aggtcaacgc
     7741 cgactggcgc gtgctgcctc agtggacggt gggcgcgcag gtcagtgcct acgccagcca
     7801 gtacgtgcgc ggcaacgaga acaacaagca ccagcccgac gggaacgatt tcaacggcaa
     7861 gggcaagatc gggggctacg cgctggtcaa cctgaccaca agcctgaatc tcggcagcgg
     7921 ctgggaactg ttcgccaagg tcaccaacgt gttcgaccgc gaggtcgcca ccgccggtgc
     7981 cttgggcgag aactcgttca ccggcaatgg cagcgagttg caggcggccg tgtccgatca
     8041 gttcgtcggc ccgaacgcgc cgcgcgccgg ctggatcggt ctgcggctgc gcttcgccgg
     8101 catcggggaa tgaccctgcg cggctgaggc gagcggcccg cagcgggtcc cttggttaga
     8161 ctgcgagcgg ctccgccagg tggccgctcg cttcatttgg cgagccttcg ttttgcttga
     8221 acaaggtgtc gcatggatcg aatcgacgcg ctgctggccg acgggcgtca gcgggcgctg
     8281 gccctggaag tcgaggtcgg caaggcggtg atcggacagt cgcgcgtgat ccggctgatc
     8341 aacaccgccg tgttcgcccg aggccacgtg ctgctgcaag gcgacgtcgg tgtcggcaag
     8401 acgaccctcc tgcgcgcgtt tgcgcaggta ctgggcggcg atttcgcgcg caccgagggc
     8461 acgatcgacc tgatgcccaa cgacctgatc tattacacct acatcagtgc cgatggacgg
     8521 ccggcggtcg agcacgggcc gctgatcaag cacggggaag acctggcgat cttcttcttc
     8581 aacgagatca accgggcccg cccgcaggtg cattcgctgc tgctgcgcgt gatggccgag
     8641 cgcagcgtgt cggcgttcaa ccgcgagttc aggtttccgc acctgctggt ctttgccgac
     8701 cgcaaccggg tcgagcgcga ggagactttc gagatctcgt cggcggcacg cgatcgcttc
     8761 atgatggaga tcaccatcga cgcgcccgcc accgacgccg acagacgccg gctgatcttc
     8821 gatccacgct tccacgacac cgacaggctg gtggggtcgg tgcaagccgc gatggttgcg
     8881 taccgcgagc tcaatgaact ggctgcgacg atccaggcga cggtgcaggc ctcggaaacg
     8941 ctcgagacct acgcgatgca cctgtggcgc gcgacagcgg tgccgggcga atacggcatc
     9001 catatctccg acatcgacat cgaccagctg atgctggcgg gcgccagccc gcgcggtatg
     9061 agcatgctgc tgcgtgcttc gcgcgtggcc gcgtggcttg aaggtcgcag ctacgtcacg
     9121 cccgaggacg tgcaggcggt cttcgtcgag acgatcgctc acaggctctg ctaccagccc
     9181 gtgttcgagt tgcgccggca ggagatctca ggatcgctga tggccggcat tctcggcagt
     9241 gttgcggcac cttgatccaa gcgcgcccat ccggcgccat gccggaaatt cactaccgca
     9301 tcggtgcgcc ggcgcgcggg tattttccgg gccatcaccg cagcaagagc ggcgagagcg
     9361 gcttcgaatt ccgaggccac gcgtcgctgc tcgatgcacc cgacccgcgc cgcctcgacc
     9421 tgcatgccag cctgcgcgac ccgttcggca actggatcgt gcgcgtctac agccagcgca
     9481 agtcgattcc ggtggccgtc gtggctgatc tgtcggcctc gatgggtttc gagggcgcgc
     9541 gccgcaagct tgacgtgctg gccgacttca ccgacagcct ggcgtggtcg gtctggcgca
     9601 ccggagacag cttcggcttc gtcggctgcg atgagcaggt gcgtgccgac ctgctgctgc
     9661 cgcagacccg ggtccgtggc gtcggtcagg cactggccgg caggctgcgc gggctgagca
     9721 tcgaaggccg ttcggcccac ggattggtcg acgcacaccg ccatctgagc cggcagcgat
     9781 cgctggtctt cctgctgtcc gactatcacc tgccactggc catgctgcga caggtcctgc
     9841 gcgacctggc acagcacgac gtggtgcccg tggtggtctg ggatcctgcc gagttcacgc
     9901 tgtctgcgcc gcgcgggctg gcctgcggca tcgatcccga gagcggcgaa cgccggttga
     9961 tctggtggag accggcactg cgtgaaaagt gggcttccgc acttgcagcg cggcgccacg
    10021 atctgcaggc cctattcagg gccatgcgct tgaagccgct attcatcgag cagggcttcg
    10081 atgcggatgc cgtgacacgg cacttccatt catgagcgca ctgccgggtc accgccaacg
    10141 atcacgccgg gtcatcgccg ccctggcgct cgcagtggcg gtcctggcga cgccccgtgt
    10201 cggcgccgcc agcgtcgacg acccggggcc cgcggcctgg cagatcgaca ccgtagagcc
    10261 tcgaccctac ggtcacacga tcggtgatgt cgtccagcgt cgagtggtca tccacgtacc
    10321 tgccggtcac acgctggacg aggaaacgct gccgagtgcc gggcaccgca gcggttcgat
    10381 cgaactgcgc cggctcgccc gcgagctgga tcgcgacggc agtggaagcc gcctcacgtt
    10441 gacgatgacc taccaggtgt tcctcgcgcc tcgcgaggtg cgcacgctgg agctgccgcc
    10501 cttcgtgctt ggattcaagg gggcgcagca ggcgcgcgac gtccgggtcg aggcctggcc
    10561 actgacggtt tcgccgctgg tcccgctcga cgtgtcgcct cgtcgcggcc tgggcgaact
    10621 gcaacccgat gcacagcccc cgctgatcga cacccgtgca ggccggtggc gactcgccgg
    10681 gtacggcgtg atcgccgcgc tgctgctcgc ctacctggcg ttggtgtatg tcggcctgcc
    10741 ctggttctcg cggcggcaac ggccgttcac gcaggcctgc cgcgcactga aggccatggc
    10801 cggacagtct cgtgccgacc ccgcctcgcc ggaacagcgt cgtcaggctt tccagcacct
    10861 gcatgacgcg ctgaatcaga cggccggcga ggtggtgttc gagcagaaca tcgagcgctt
    10921 catcgccgca cagccgcggt tcgccggtct gcgcgaggat ctggtcttgt tcttccggca
    10981 ctcccgcgcc gagtttttcg ctggcgccgg ggcggcaccg gatcgcgcac tgcccgccgt
    11041 ctggctgatc aacttgtgtc gcaagtgccg cgatgcggag cggggtgcag catgaatttc
    11101 gacacgccct ggctgctggc actgttgccg ctcgcgttgc tgccctggtg ggcaaggccc
    11161 cggcacgcac tgtccaacgg ctggctcgcg atgatgccgc gcgaccgcgc ctccgatgcg
    11221 atcagcttcg cactgcgcgc ggcgtcggtg ggcgcgctgg ccgcactgat cgtcggcgcg
    11281 gccggaccgt atcggcccga gtacacggta cagaaagtgg gcaagggtgc cgagatcgtg
    11341 ctggtgctcg atcgcagccg cagcatggac cagggttttg ccggtggggc cggcggcccg
    11401 ccaccgggaa cccgtcccaa cagccccgag gctctcgagt actacaccag ccgcaacgca
    11461 gccgccagcc gtgactcgaa aggaaaggtc gcccggcagc tgctgtcgga gttcgcggca
    11521 cagcgtcccg aggaccgctt cggcatggtg ctgttcagca ccttgccgat ccgcacgctc
    11581 gagttcacgc agaagcccga ggcgatccag gccgccatca ccgcaggcaa catcggacgc
    11641 gggctggccg acaccgacat cgggctggcc ctgagttcgg cgctgtcgta cttcgacgac
    11701 cggccctaca ccggctcgcg catcctgatg ctcgtgtcgg acggtggcga caggatcgac
    11761 gtcgacgtgc gcgagcgcgt ggcgtacctc gcgcgcaagc accgggtggc aatctactgg
    11821 ttctacatcc gctcgagcct gagccccggg ctgatggccg acgccagcga gcccccgtcc
    11881 aacgtggaca ccgttcccga gtacttcctg caccgattct tcgcctcgct gggcacaccg
    11941 tacaaggcct acgaggccga gaacccgcag gcgttgcaga aggccatcga cgatgtgaac
    12001 cggctggaga acctgccgat cacctacctg gacaccgtgc cgcgtcggga cctgtcccag
    12061 ctgggctacg gcatcgcgct ggcctgcgtg ctcgcgctgc tgggcgccaa cctgatggag
    12121 atccggcgat gggcctgagc tcgcggatcg cgcactggcc cagtcgccga cgcacccgcg
    12181 cggtgatcct cgtgctggcg ctgctggcgc tcgtcgccgc cgtcgacggc gcacgcatcg
    12241 cgcgtcagca cggcatcaac gacgcgatcc gctcggggca actgccggca gaagcgccgg
    12301 gacagccacg agagatccag ttcgcccgtg ccgctgccgc cgcacccggg cccggcggat
    12361 ccgaggagga cgtgctcaac cgctatcggg ccctgcagga cgacaccgcg ctgggccgtg
    12421 ccgcacgctt caacaccgcc aatgcgctga tgcggcaggc catcgccctg cggtccaccg
    12481 cgcagccggg ccaggccatc ccgctgatcg agctggcgaa ggagacctac cgcgagttgc
    12541 tgcgcgccga cccggcaaac tggggcgcac gctacaacct cgagcgcgcc cagcgcctgc
    12601 tgcccgaccc cgaagacagc gatgctccgc cgtccgagcc ccccagcaat gcggagcgtg
    12661 cggcaacgac gatgcgcggc tattcgccag gcctgccatg acgagggagt tggttcgatg
    12721 tcgtggctga ggcgaggcct tgcggcctgt cgtgtgtcga tccgcggggc attcacccgc
    12781 cacctcgacc gcacgctgct gatcgttgcc tcgctggcac tcgcggcaac cttcctgaac
    12841 ccggtcgcca ccgtggaccg cgccttgttc gatcaggtga tcgttctcga catcacgcag
    12901 agcatgaacg tgaccgacta ccagctcgaa ggcaagccgg tgtcgcgcct gagctatgcg
    12961 aagcacgcgc tgcggcagct gctgctcgaa ttgccctgcg gctcgaaggt gggatggggg
    13021 gtgttcaccg agtaccgatc gttcctgctg atcgcgccca tcgaagtctg cgagaacctc
    13081 aatgaactgc gttccacgct ggacaacatc gatggccgca tggcctggac cggcaacagc
    13141 gaggtcgcca agggactgtt cagcgggatc ggcatcgcca ggcaattgca ggacaagccg
    13201 tctctcgtct tcgtgaccga tgggcacgag tcgccccccg tgaacccgca gcatcggccg
    13261 gtcttcaacg acaagccggg agtggtgcag gggctgatcg tcggcgtcgg gggcctcagg
    13321 ccgtttccga tcccgaaact ggatccactg ggtcgcccga tgggcctctg gggcgccgat
    13381 gaggtgatgc agaccgaccc gcgcagccag gggcgagggg gcagcgtggg ccacgagcaa
    13441 atggtcgagg atcctgccga tgccggaaag gcggccaacc tgcccggcag cacgccgggc
    13501 agcgaacacc tgtctgccat gcgcgaaggg tatctgcgcc tgctggccga cgacacaggc
    13561 ctgtcgttcc tgccactgca gcagccggaa gccttgatgc gcgcactgac tgcgccgcag
    13621 ctggcgcagt cagtgccggc agcgacggat ctcagggtgg cgctggcggc cctggcgttc
    13681 gtcctgctgc tcgcacgcag tgcgatggcg gtgtggcagc gttttgaaac aagacaaaga
    13741 tcaccccgct tgggcaggaa gccctcagtg cgaggacaat aagggttttc gttggcacgc
    13801 ccacacccgt ggctgccggc tcaacatgag aggctcactc ccgcgccatg gccacacaac
    13861 gaaccaagac cccagcttcc tccacagcgg ctgcggcagc cgccagcgcc gacagcgtca
    13921 agctcaggca gacccaggcc gaatactcgg ccgcccgacc gtcgtcggaa cccggcatca
    13981 agccgatgat gtcgagttcg aagatgtatg tctgccggcg ctgccaggcg aacttcaaga
    14041 ccggcgaagg caagcccgac ccgcgcttgc ggggcctggg caggtgcaac aagtgccttg
    14101 atgcggccac cgcatcgcag agcgccgcac tggccgcctg aacccgcaca gcaggctctg
    14161 gcgcctgcaa gcacactgca tcgatccgca actgcggatc tttgcccacc ccacatatgc
    14221 cggtctatat atcttatgtc tactgatgga ctcgctgagg agtgcggcag gcaccgcgcg
    14281 agtcccggcg gcgacacctg ccgatccttt gtgcggcggc cttgagccct gtcaaaaaag
    14341 acgccgaatc cagcgagcga ctgaccggac tgcgcgaaga tcgctcttcg ctggtgcagc
    14401 cttgaggctg cgccctccgt gtcccacctt cggctactcg catggacagc ctgtccgcca
    14461 tcgcatacgt cagttcagcg cttggagatg tctccgaggc ccggctggaa gcgctgcttg
    14521 tcgattcgcg aggcttcaat ctctcgaagg gagtgacagg cgtcctgctc tatcacgacg
    14581 gcaagttctt ccagtacatc gaagggccgg tcagcggggt cgaggaggtc tatgcccgca
    14641 tcaaggcatc gcgccggcat ttcggtctcg tcgaattgct ccatgagcga attcacgggc
    14701 gggtattcag cgaatggagc atggggttcg cctgtgctcc acgcaacttc atcctccagc
    14761 tggaacggca acgctggtcg gacgtgtcgg cccagctgac gtcgggcgct gaagtttctg
    14821 ccggcctgga cttcctgctg cagttcttga agggcagtac ccgcggcatg tcgtgaacgc
    14881 aggctcgagc cccaactcga cggtccaggg gaaacctctc cacgaacagc ccaagtgccc
    14941 cagcacgggt ctattcgaag acatacgatt ccatcgacaa catgccatag agcgtgccgc
    15001 cttcgatgac gtcgacccgg tcggcatccg acgtggcacc caccgcgacg agcagcggaa
    15061 ggaaatgctc ctcggtcggg tgcgaacgca ccgcgtgcgg cgcccgatgt cggtaatcga
    15121 ccagcgcggc ggtatcgcgt tccgcgacag cctggcggat ccactgcacg aagtcggcga
    15181 cataacctgc ctcggtcgcc gggccccgga actcgcccag gttgtgtgtc aggctgcccg
    15241 agccgacaac cagcacgcct tgctcacgca acggacgcag cgcctctccg agccgcaggg
    15301 caccccgcgc atcgagcgtg tcgctcaggg acagctgcag caccggcgtg gacgcggtcg
    15361 gcagcagatg gcgcagcgga acccaggctc catggtcgag tccgcgctgc gcgtccagcg
    15421 tgacatcgat gcccgccgaa gcgagcagcc gcacgatttc cgcggcccgt gccggatcgc
    15481 ccggcgccgg gtactgcaac tcgtacaagg ctggtgggaa gccgctgaag tcatggatcg
    15541 tggcgggccg ggtgccggac gtcacgcgaa agccggtcgt gatccagtgg ggagacacga
    15601 tcagcaccgc cgacagcggc ggcaagcggc ggccgagcgc ggacagtgcc gcaccagcaa
    15661 cgccgggctc cagggcaaag gtcggtgcgc catgcgagac aaacagggac ggaacggttt
    15721 tcatcgggag ctccacggtt gaacctacag tcggggcaca ccccgcgttt tcaacgcggg
    15781 ccagactgac ttccggatga atctgtgagc gttcacctca cctctgcgcc agacctgtgc
    15841 acggcacgcc cccgcctgtg gggcgaactg tgcaccaccg acttcgcgac gctggatccc
    15901 gacaccaccg tcgccgtgct gccggtgtcg gccaccgagc aacacggccc gcacctgccg
    15961 ctgtcgacgg acgcggtcat caacagcggc gtgctgcatg cagcgctggc gcgacttccc
    16021 gctgcagccg cgacctcgat cctgcaactg ccgccgctgg cggtcgggga cagccttgag
    16081 cacagcgcct tcccgggcac cgtgagcgtc gacagcgacg cgctgatggg actgtggctc
    16141 tcgatcgggc gcagcgtcgc acgtgccggc gtgcgcaagc tggtgatctt caacagccac
    16201 gggggccaga aggcgcacgt cgatctggtc gcggtgcggc tgcgggtcga atgccggatg
    16261 ctggtcgtgc gcgcgcacag tttcggcttc ggtgtgcctc caggcttgtt cgatgtcgag
    16321 gagcgcgccc acggcatcca cgccggtgcg gtcgagacct cgctgatgct gcacttgcgg
    16381 cccgacctgg tgcggcaatc agcgctcaag accttcccgt cgctcggcca gcagctggtg
    16441 gcccggggcg gcacgctggg cgtcgaaaag ccggtcggct tcggatggat gacgcaggac
    16501 ctgaaccccg aaggtgcctg tggcgatgcg acgcaggcga gtgccgcaaa gggagctgct
    16561 gtgctcgatc acatggccga gggccttgca aggctgttgc aggacgtgat cgcgctgccg
    16621 ctggacaccc tgcgcgacgg accgttgccg acaggctgat cgcaacgcct gagcgccgcg
    16681 cgcaagcgac ctcggctcac tcgggcttga ggtgacccgc ccgctgctgc ttggtctcga
    16741 tgtagcgctc gttgaagggg ttcaccgggc cgtgcagcgc cagacgttcg acgacctcga
    16801 tgccgccggc ttccatggcg gcgatcttcc gcgggttgtt ggtcatcagg cggatgcgtg
    16861 cgatgcccag cgacttcagc atcgccaccg cagcgccgta gtcgcgatcg tcggcagcga
    16921 atccaaggta gcggtcggcg tcgatggtgt cgagcccgtc atcctgcaac cggtaggctc
    16981 gcagcttgtt cgccagaccg atcgagcgcc cctcctgttc gaggtacagc agcacgccgc
    17041 ccgtctcggc gagtcgttcg atcgcgcggc gcagctggtc gccgcaatcg cagcgcaggc
    17101 tgccgagcag gtcgcccgtg agacaggacg aatgcagccg aaccggcacc ggctggctga
    17161 aatcgggttg cccgacgacg atggccacgt gctcggcacc gccgtggtcc tcgcggaaga
    17221 gaacgagttc gcagtcgtcg tgcgccgcaa gtggcacctg cgcgtcgctg acgcggcgca
    17281 ggctcgcgcc accaccggcc ggcaccttcg agaggtcggc gctggaaacc caaagcaatt
    17341 gctcggcggc cagtgccggt tcgagtgccg cgggaacgtc gacgaccagc agtgccggga
    17401 tcaaccgggc ccgccgcgcc agctgcaggg cactgtccat cgcaaccgcg cgcaccggcg
    17461 gcgtccgctc actcgctgcc cattccggca atgcagcaac cgcggacagt ccgctggacg
    17521 tcaccgctgc cagccccagc aaggcgtcga gcgagatgtc gtccggcagc cgcagccgca
    17581 gcgaggtggc cgcaagcgac cctgtcgaat ccgcctcgat ccccagcgcg agcgcgcgtt
    17641 cgatcgtcag cagcaggtgc accgaagctc cgagcgacga catccagcgc agccgcggct
    17701 cggtcaaagc ctcgacagca gcgaccacgc agctgtcagc ggaggcttcg cccggcttgt
    17761 cgcgatcgat caggcggatc gcgcggccgt gtctgagttc ggtctgcgcc cggtcgatcc
    17821 gcgtggcggc ttgctcttcg gcggccgagg ccggcgttgg cgtgcgatgt tcctggtatc
    17881 gatcgttcat caagaaaggc gttggacgca agcgcctggg ctgatcaggg cgagcagaac
    17941 agatgcggga gctgcgttcc agacgagcac tccgcaccgg aacaggcgcc tcgattgtcc
    18001 cccgagcagc gaagctcgtt tgtcgatgtg gcatgaagct atttggaaat cagccatgca
    18061 cgtagatctg tccatccctg ggcaatcgag caacgcgaag ccatcaccat gaattagcgc
    18121 aacaatcgtt ggcctgataa gttttatggg cgcaaccaac aaccctcgag agcgacccgg
    18181 atcccggagc ccctcgcacc aacaattcaa cggagaccca caacatgacc atcaagcaag
    18241 gcgatcgcgt tccaggcggc agcttcaagc aactcaccac gaccggcgtg gtggacatct
    18301 cgaccgactc cctgctcaag ggcaagaagg tggccctgtt cggactgcct ggcgcctaca
    18361 ccccggtgtg ctccgcgagc cacctgccgg gctatgtcga caacgcggag aaactccgca
    18421 aggccgggtt cgacaccatc gcctgcgttt cggtgaacga tcccttcgtg atggacgcct
    18481 ggggcaaatc caccggtgcc gacgacaagg tgctgatgct ggctgacgcc gacggctcgt
    18541 tcaccagggc catcggcctg gtcatcgacc tgccggcttt cggtctggcc ggccgctcgg
    18601 aacgctactc gatgatcgtc gaggacggcg tcgtcacccg gctcgaagtc gagaagagcg
    18661 tgctcgatca cgacggcagc agcgcggcct gcatgttgcg caacagctga tcgaatcatc
    18721 gctctgcaga aagcccgctt cggcgggctt ttctatttca aatttattgc acccaagatc
    18781 gccatttgtt gatcaggatt tacccgcata gagcgtgttt ccctatgcat ttcgggaacc
    18841 gcgaacgtct aaatcgttgg tacgggattc ttgtttcatt gaactggaat ttcccgtcct
    18901 gcagggccga cgaacgaccg gccccccctt caaaccctca ggagaccgct tcatgaccct
    18961 cagataccgc gatcgcatcc cgatcgttca tttcaaaaaa atcgtcaccc acgtcgtacg
    19021 cgggatcacc tcggaacaga ccttcaacgt cgagtaggtt gcaccgttcc gcctgcccgg
    19081 cgccttcaaa ccgatctgtc cggcacaccc ccctgccgga ctccatcacc tcaatgccta
    19141 aggatgacaa atgactttca ttacttctgc cgacgccaaa gaactcttct ggagcgagat
    19201 cggcgtcgcc gacaagttca agccccagca cacgaagttc ctgaagtacc tcgaaggcca
    19261 cgactccgtg acgtacgagg acgcgatgaa gcaacttgac agcttgatcg gcgagttgac
    19321 gccgaatgac aacccgccgc gacgcatgct gatgtcccag cccggcgcac gctaggggcg
    19381 caggcgagcg ggacgctcgt cggcccgctc gccgcccttg ttcgcgcccg gatcgctgcg
    19441 ttcggtggtc acttcatcgc gccgagctgg ttgttctgcg tggcgcctgc ttgcaggtgc
    19501 tcagtcggga agccccagca cctcgatcac ggcgcccaca tccagctggt tcgccaccct
    19561 gtcgggcgtg gctgtcacca tgtaatcgat cacgccgagc aacggggcgc cgtagcggcg
    19621 ttgcagcgcg cgtctgagcg tgtcgatgtt gtcctgcgca tgggccatgt ccggctcgac
    19681 acggttggcg atccagccgg ccaggcgcag tccgcgcgcg ttgatggccg cggcagtcag
    19741 caggctgtgg ttgatgcaac ccaggcgaag cccgaccacc agaatcaccg gcaaagccag
    19801 cgcactcgca aggtcgtcgg caccccagcc tccggcatcg gcgccgtcct cgacctcttc
    19861 agcctcgacc agcggcacgc agaatccccc gacgccctcg accaccagga catcgcagcg
    19921 cggcatcagg cgccgtgcgc cggcggtcag cacccgcggc tcgatgtgcc cgccttcgag
    19981 ccgcgccgcg aggtgcggcg cgcaggcttc ctcgagcagg caggggcaga cctcgctgcg
    20041 cgtcagctcg atgttgctcg cagcgagcaa ggcctccaca tcctcgttgt gccagtgccc
    20101 accggggcca ctggccgcgc tgcaccacac ggcacccgca gacacgggct tgtagcccgc
    20161 gacgcgcaga ccttgtctgc ccagcgcatg cagcaggccc gcactgaccg tcgtcttgcc
    20221 gacctcggtg tcggtgccgg tgacgaagca gccgcgcaat ggacggtcgg tgtcgggctc
    20281 ggccaggtcg gccgcacgtg cttcgggttc ccacaggaca ccggcctcgg tcgcctgcca
    20341 accattcgat ggatcgccgc cagccgtcat catgtcgccc ctcccggttc gcccgccgcc
    20401 cgggctgccg ccgccaggcc ggacaacagt cgcttgacat cgtcatgccg atgcgcggcg
    20461 ctgagcgtga tgcgcagccg tgcgctgccc gagggaaccg tcggcggacg gatcgccggc
    20521 acccagaggc cgcgcgattc gagcccggca gcgaccgcca gcgcctcggc actgccgccg
    20581 acgatcaagg gctggatcgg ggtctgcgat tcggccaggt gccagccccg ctcgagaagc
    20641 acatcgccaa gcacctcggg cacgccctgg cgcagcgccg cgatgagttc gcgcaggtgc
    20701 agccgccgct gctcgccttc gggtccgcgg atcaatctca agctcgcacg cactgcgtgg
    20761 gccacggccg gcggggacgc ggtggtgtag atgtaggcgc gcgcgctctg cacgagccac
    20821 tcgatgacga cgcggtgcgc cgcgacgaag gctcccgccg cgccggccgc cttgccgagc
    20881 gtgcccatgc agatcagtcg ctggctgcgc agcccgaagt gcgacagcac gccgcggccc
    20941 tcctcgccaa gcactccgaa gccatgcgca tcgtcgacga cgatgtaggc atcgtgccgc
    21001 tctgccagcg cgagcagttc cggcagccgt gccagatcgc cgtccatgct gaacaccccg
    21061 tcggtaacga tcagcttgcg cggggtactg catgccgcga gccgcctctc gagaccaccc
    21121 agatcgttgt gcggataggt gtgcactgcc gccttggcaa gccgcgcgcc gtcgatcagc
    21181 gacgcgtggt tcagcaggtc cgtgtagatc gtcgcctgcg catcgcccag cgcggtgagc
    21241 acggccatgt tagccatgta gcccgtgcag aagtacaggg ctcgcacctc ggggaggtgt
    21301 ggcgacatca tctcggccag atcgtcttcg agcagcgcgt gggcacgcga atgcccgctg
    21361 atcaggtgcg aagcgccgct gcctgccccg tagagccgcg cgccttcggc cagcgcatcg
    21421 accagtgccg ggtggttggc aagacccagg taatcgttgc tgcagaaggt gagcagttcg
    21481 cgcggcgcgc agtcgttcga gctgaccaac tggtggggcg cacaggcgct ctcggcgatg
    21541 cggcgctgcc gacgcagcga ttgagcttca cgctctgcca gctgttgttc gaggtgcttc
    21601 aacatcgcgg cgggtatcgg tgggtggtgc ggccgtcagt gtcccgcgct cagggtggca
    21661 ttcaaggtcg caacagtgcg atcggcaaga taagccacgt gctcctcgtt cagcacgtag
    21721 ggtggcaaca gatagaccgt gcgcccgatc ggtcggatca gcaggccctg cgtgcgtgcg
    21781 atcaggtgga agcgctcggc gaaacgcacg ccggcgtgcg cctcgcgcac atcgaacgcc
    21841 cagatcatgc cgcgctggcg cacatgctcg atgcgcgcat cgccggccag ccgcgcggcc
    21901 agtgcggcgg tgagcagcgc gcctgtgtcg cggttgcgct ggagcaccgg ctcctcgtcg
    21961 aaccggtcga gcacggccag cgcagctcgg caggccaacg ggttgccggt gtacgaatgg
    22021 gagtgcagga aaccccgggc ggtgtcgtcg tccaggaagg acgtgtagat cgcatcggtg
    22081 gacagcacca gagacagcgg caggtagccc ccgctgatgc ccttggacag gcacagcagg
    22141 tcgggccaga tggcgggttc gcctaccgcg gcggcctgtt cgaacgcgaa gaaggttccg
    22201 gtgcgtccgc aacccaccgc gatctcgtcg gcgatcaggt gcacctgata tcggtcgcac
    22261 agcgcccgga cctcgcgcag gtaggctgga tcgtgcatcg ccatgccggc ggcacactgc
    22321 accagcggct ccaggatcac cgccgcgatg tggccggcgc gcgcctcgaa cagcgttgcg
    22381 agctcggcgg cggcacggtg tgccacatcg gcagccgact cgccgggctc ggcctgccgt
    22441 gcatcgggtg ccatcaccac gtgggaccgc atcagcagtg ggtcgtacgc gtcgcgaaac
    22501 accgccacat cggtcaccgc gagcgcgccg atggtctcgc cgtggtaacc atggcgcagg
    22561 cagacgaatt cgcgccggtc gccctgcccg cggttcttcc agctgtggaa gctcatcttc
    22621 aaggcgatct cgaccgccga ggcgccgtcg ctcgcgaaga aggtgtgccc tagcgcctgg
    22681 ccggtgcgcg ccgacagccg ctcggccaac tcgaccgccg gcgcgtgggt gcagccagcc
    22741 agcatcacat gctccagcgt gtcgagctga tgcttcaggg ccgcgttgat gcgctcgtcg
    22801 gcatggccga acaggttgac ccaccacgag ctcgtcgcat cgaaataccg cttgccgtcg
    22861 acatcctcga gccacgggcc ctgagcgcgc gcgatcgcaa tcggcggcac ggcgtccatc
    22921 cgctgcatct gcgtgcacgg atgccagacg ctcgccacgc tgcgacgctg ccagtcggcg
    22981 tgggttgagg aagcagaacg gtctgtgggg gtggacatgc ggggcgccgg gcagggtgag
    23041 catcgattat cgaagcccgg cccgccacgg cattttccta gggttgtcac ctacgtgtca
    23101 gcccgcgagg actaccatcg gacgcggggt accgctgcag cgtcgctgcg tcaggcaccc
    23161 gctcccacct gtcccggagg cctccgcttg gacaagcact acctgacacc gctgttcgcg
    23221 cccagctcca tcgtcgtgct gaccggcggc gcggcgcccc agactgccgc agcacgcgca
    23281 ttgcacgctg cgctgaaagc gcaggccttt gccggcacgc tgacgttcct ggacgtgaat
    23341 gccacgggca cgttgtcgga tctggcgcac gcccgagccg atcttgccgt gatcgcgatg
    23401 ccacccgacg aggtgctgac ggcgctcgcc gccgccggac gcatccattg ccgtgcggcg
    23461 ctggtcatct cgagcaacgt cgatgccgcg acggtggccg agatgcacca ccaggcccgg
    23521 ctcgacggca tgctgatgct cgggccgaac gggctcggct tccagcgacc cggcctgcaa
    23581 ctcaacgcca gcatcgccgg cccgctgccg gccagcggct cgctggcgct cgtgtcgcaa
    23641 tccggcgccc tgacgtcggc gatcctcgac tggggaaatt cgaacggggt cggcttctcg
    23701 accgtggtct cgctcggacc ccatccttca gtcgacctgg ctcaggtgct cgatttcctg
    23761 gcctcggacg cccagacgca cagcatcgtg gtctacctgg agggcatcac ccacgcccgc
    23821 cgattcatga gcgcgctgcg cgccgccgcc aacgccaagc cggtggtggt gctgaaggcc
    23881 gggcgccagc ccgccgggaa cgccgcggcg ctgacgcatt ccggcgcgat cgtcggcaac
    23941 gacgatgcct tcgatgccgc tttgcgccgc gctggcgcgg tgcgggtgcg ctctttcgtg
    24001 cagctgttct ccgctgccaa gtgcctggcc tctcgctatc gtgcggtggg gccgcgcctg
    24061 gccatcgtca ccaacggcgg tggtcccggc gtgatggcag ccgactgggt cagcgagatc
    24121 gggctgcaac tcggtcgcct gagcccggcc gaggcagaca ccctgcgccc cagactcgcg
    24181 cccggcgcca cgatgagcga cctgatcgac ctggccgagg acgccacggc ggagcagttc
    24241 gaggcggcga tcgctgccgc gacgcagtcc acgcagatcg atggcgtcct ggcgatctat
    24301 tcgccgaaga ccggcaccga ttcggcggcc atcgcacgcg ctctggtcac ggtcaagaag
    24361 cgctcggaaa agcccctgct gagctgctgg atgggtgacg cctcggtcgt ggagggacgg
    24421 cgcctgctca gcgatgccgg aatcccgacc tttcgcacgc ccgaatcggc cgtcggcgcc
    24481 ttcggcaaca tcgcggccta ccaccgcaac cagcagctgc tgcagcagac gccgccgccg
    24541 ctgtccgatc tggccaagcc ggacgtcgag ggcgcgcgga tgctgatcga gagcgtgctc
    24601 accgagcggc ggcaggtgct gaccgaaatg gagtcgaagg ccctgctcgc ggccttccac
    24661 atcccggtga ccaagacgct gctcgcgcgc agcgtgcacg aggcggtgct gatcgcgacg
    24721 cagctgggct acccggtcgc gctgaagatc gactcgccgg acatcagcca caagtcggac
    24781 gtccagggag tggtgctgaa cgtggtcaac gcgatcggcg tgcgcgacac ctacaacgag
    24841 atgctgcgtt cggtgacccg ccagctgccc gaggcgcgca tcaacggcgt gaccatccag
    24901 aagatgtcgg gccagcagcg cggtcgcgag gtctgcatcg gcctggtcac cgacgagccg
    24961 ttcggccccg tgatcacgtt cggcgcgggc ggcaccatgg tggagctgat cgacgaccat
    25021 gcgatggaac tgccgccgct gaaccagttc ctggcgcgac ggctcatcga gcgttctcgg
    25081 gtcgccgaaa cgctcggcgc ctggcgcggc gcggcgcccg ccgacatgga atcgctggag
    25141 caggtgctgc tgcgcgtgtc ggagatggtc tgcgaactgc cgcagctgcg cgagatggac
    25201 atcaatccga tcatcgtcga cgagtccggc gccctggccg tcgatgcgcg catcgtcatc
    25261 gcccagagac ccgaatcgac cgccgcgtcg aaccaccacc agtaccacca cctcgccatc
    25321 ctgccctacc cgtcgaacca cgcgcagctg tggccgctgc cgggcggtgg cgagtacacc
    25381 gtgcgcccga tccagcccga cgacgcgacg atgctgc
//
LOCUS       DAOORV010000044        24926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2404_length_24926_cov_7.544369, whole genome shotgun sequence.
ACCESSION   DAOORV010000044 DAOORV010000000
VERSION     DAOORV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_24926_cov_7.544369"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /gene="clpA"
                     /locus_tag="PKV56_07930"
     CDS             complement(<1..2487)
                     /gene="clpA"
                     /locus_tag="PKV56_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOX67783.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLMALLDNPSAAEVL
                     RACSANVDDLRKSLAQFIKENTPTVGGTDEVDTQPTLGFQRVIQRAIMHVQSTGSGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIKKSDPPEAAKPSDSGSS
                     GDSEKDDSDGKGSPLEQFTQNLNQLARDGKIDPLIGREHEVERVIQILCRRRKNNPLL
                     VGEAGVGKTAIAEGLAWRITEGDVPEVLANATVYSLDMGALLAGTKYRGDFEQRLKGV
                     LKVLKDQPNSVLFIDEIHTLIGAGAASGGTLDASNLLKPALSSGAMKCIGATTFTEYR
                     GIFEKDAALSRRFQKVDVVEPSVEETIEILKGLKSRFEEHHSVKYALGALQAAAELSA
                     KFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTITRNEVEEIVAKIARIPPASVSND
                     DRGKLKSLDRDLKSVVFGQDPAIDALAAAIKMARSGLGKPDKPIGSFLFSGPTGVGKT
                     EVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKKPHAV
                     LLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKADFRNVIIVMTTNAGAETMNKATIG
                     FTNPRERGDEMGDVKRMFTPEFRNRLDAIVSFRALDEEIILRVVDKFLLQLEGQLAEK
                     KVDVTFTDALRKHLAKNGFDPLMGARPMQRLIQDTIRRALADELLFGRLVDGGRLTVD
                     VDADGKDSAQVLALAYLNSYFESGLKSPLDDAILAHEDIDVSGWVKVDEVPFDFERRR
                     VSVLVERAGMRQLVVKGAPEDVVQLCTHYLD"
     gene            complement(2484..2837)
                     /gene="clpS"
                     /locus_tag="PKV56_07935"
     CDS             complement(2484..2837)
                     /gene="clpS"
                     /locus_tag="PKV56_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOX67784.1"
                     /translation="MSTDKPASKPPVVGSPRDDGQGAVVVERQVQKTEPPRMYQVILL
                     NDDYTPMEFVVMVLQEYFNRDLEAATQIMLKIHHDGRGVCGTYSKDVAATKVELVLAA
                     ARRGGHPLQCIMEAA"
     gene            3071..3343
                     /locus_tag="PKV56_07940"
     CDS             3071..3343
                     /locus_tag="PKV56_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOX67785.1"
                     /translation="MSTGTVKWFNDAKGFGFIEPDGGGDDVFAHFSAIQMDGFRTLKQ
                     GGKVTFELVQGPKGQLAQNIVPELGLSEHVPESQRDQTSPAQSLAH"
     gene            complement(3468..3812)
                     /locus_tag="PKV56_07945"
     CDS             complement(3468..3812)
                     /locus_tag="PKV56_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67786.1"
                     /translation="MSPVRSTTLIALLLAAATSVAYAETAASPAAEQRFDNRQDRQEE
                     RIENGVSSGALTKPEARRLGREQARLTRAEARAEADGKVTRKEAVVLEKRQDHASRHV
                     YRAKHDAQTRKP"
     gene            4038..4469
                     /locus_tag="PKV56_07950"
     CDS             4038..4469
                     /locus_tag="PKV56_07950"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HOX67787.1"
                     /translation="MKRGLLFLMAMIWPLWGLAAAPDVPDPEAWVRLSPAEQAARRAE
                     LKQKLQQATPQERAAFRNRLRERLEEMPPEQRQALAERTRERWQKLDPQEKQRLINER
                     RERVKAMSPEERRQLIEQRREILGKLSPEERALLREKLTGP"
     gene            4486..5139
                     /locus_tag="PKV56_07955"
     CDS             4486..5139
                     /locus_tag="PKV56_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX67788.1"
                     /translation="MDSTRMDAVSRLTGWLAGAAGRMRPGEPAQGGDWALWREACAGN
                     PRSAQQLVRELTPSAYGLALQMLRRAEDAEDAVQEAFLRLWRSHPTDTEGAKLSTYFN
                     TIVINRCRSLLVARREDATDPDALADLHDARQVSGAHGGHHDDPAESGWADTGSALQR
                     GLMRLPVRQRMAIVMWAYADSGVADIARTLDIDPNAAHQLLHRAKQALRIHIEGAAP"
     gene            5136..5501
                     /locus_tag="PKV56_07960"
     CDS             5136..5501
                     /locus_tag="PKV56_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67789.1"
                     /translation="MTDRPPLEHRDRSDPADDQLRSRLRELRPGASAIDALGARVLVQ
                     WSEQHHHAGRTTLHLGGTMGTAEFGLGRSHRRLRASLASGLVVCAIVAAVMWAQRPDP
                     TLDELIQADVLSQMAIGEM"
     gene            5649..6515
                     /locus_tag="PKV56_07965"
     CDS             5649..6515
                     /locus_tag="PKV56_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HOX67790.1"
                     /translation="MSLPASASPTLRSRRRVLASALATGLAPVLARAASEVADNENNE
                     RWQMLRKSLFQNRTISEDSAAIIELDTPARAADAAVVPIAFRTRFVQTAGTYIKKVHL
                     VIDRNPSPMGATFTFTPESGRAEIETRVRIEEYTHVRAIAEMNDGRLFMHTRFVKASG
                     GCSAPAGKDLQAALANAGRMKLRVEGDVVLGQPTLAQLSISHPNVSGLAIDQVSRLAP
                     PPHYVRSIEVAYAGKPVLSADLDFTISENPNVRFFFVPKEAGQLTVNVIDSNDAKFEK
                     SLAVAPGQRSDA"
     gene            6508..7851
                     /gene="soxC"
                     /locus_tag="PKV56_07970"
     CDS             6508..7851
                     /gene="soxC"
                     /locus_tag="PKV56_07970"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HOX67791.1"
                     /translation="MPERGEPASTEPPGTSPDDLIPVAGGGLLHRRALLHRGLVFASA
                     APAAMLTTGASAASPPHDPVVAAASGEFPAWMRTPGTPFSTYGQPSRHESQVVRRIGA
                     NRLAPGNGVSWTPLEDLEGFITPTGLHFERHHNGVPEIDPARHTLTIHGRVKSPLAFS
                     VADLLRYPMQSQLVFIECGGNSNAGWHNVPIQRPVGSFHGMVSCSEWTGVPLSLLLDE
                     AGLDSSATWAVAEGADAIAMNVSLPVDKLMDDCLVALYQNGERLRPEHGYPLRLIVPG
                     WEGVLNVKWLHRLELTDQPVMARNETAKYTELQPDGKARQFTFVMEAKSLITSPSHGQ
                     HLPSAGYYQIGGLAWSGRGRIRRVEVSADGGRTWADAALQDPVLPRCLTRFRLPWHWS
                     GRPAILKSRATDESGYVQPERQALISQRGSNGYFHCNAIVSWAVDEDGDVTHVYA"
     gene            7838..8401
                     /locus_tag="PKV56_07975"
     CDS             7838..8401
                     /locus_tag="PKV56_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX67792.1"
                     /translation="MSMPERPHFRSLIGVLIAALASIQGGGACAAEAPGAPRLGHRLT
                     PEQAQRQSLNVFPDGRGLPAGRGTAAEGRPLFQQKCAACHGQAGRGASAEELAGGEAP
                     LTSQSPDKTIGLYWPYATTIFDFTRRAMPLSAPGSLSDDEIYALTAYLLFENRLIGER
                     EEMNPVTLPRVHMPNRDGFIGTDAQAR"
     gene            8579..9697
                     /locus_tag="PKV56_07980"
     CDS             8579..9697
                     /locus_tag="PKV56_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOX67793.1"
                     /translation="MTSTATPDESATESSPAVENDTVGQDSGRALISDLFRRSPAIYW
                     VDLLLSAGTAWALTAVYFTAPAWSALQVAAFVVASFLFFRAGTFIHEIVHMPGGQMEG
                     FKRTWNLLLGVPFLMPWVMYRNHIEHHNHRHFGTPADGEYLPLGSSPRVETLKYLAQA
                     PLLPLFMMIRFGILGPASWLHPRLREWVLTRASAAVSNPYYNKRFPKRDEAHLRVVEV
                     LCLIYLIAIAALLWLGSISPVHLFMGYLLLACTLGLNWVRNLAAHHYVNGGGRMTMAE
                     QLADSINVTGQTWLTIAMFPVGLRYHALHHMFPGVPYHHMGEAHRRLMQGLPPDSVYR
                     GCNRDSFFSAVRELWRSASHTPPEQSAMRAWVAGSGRT"
     gene            9700..10701
                     /locus_tag="PKV56_07985"
     CDS             9700..10701
                     /locus_tag="PKV56_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOX67794.1"
                     /translation="MRAAGLTAPDLTVAADAGAPPCLIVNPRSFSASRGLAQQASALA
                     RTHGAEIVNVDGPAALRAAVESILARRQRTVMVLAGDGTVCAIVDQLSNLAPGAWTPD
                     LLILPGGRTNLTAVDLGTGSRALPTLKRVLSMGRDQRGNVAVEERCALRIEQSPAPAR
                     HGFFFSAALIDSATRQCHQRRIDRNEHGPFTTLSYLLKLGVLALFGRTGLVCPTLSVD
                     AQGCGTQQGPVCLLMASTLSHRKGLFDPYAARGQGDVRMMSVSRDAPGIWRSLPRLLT
                     GRYSKAMNADNGYVSGRCTQVDISGLSGYMLDGEPFDADPDRPVTITAGRRLRFLKP"
     gene            10698..12473
                     /locus_tag="PKV56_07990"
     CDS             10698..12473
                     /locus_tag="PKV56_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67795.1"
                     /translation="MSASDKQGLTSGRSRAFPMIRAFVLAYPGRSATALGAVFLAGLM
                     DGLGLSMLLSMLSFATRTDKHEPSMPEQFALRVAEFVGLQPSAPVLLMLATVLIAMKA
                     VLSLAANRQVGYTVANIATDLRLSLIRSVLSARWSHYLQQSVGRLSNAVSTEAQRASE
                     AFQCSAEMAAMLLNSMIYLVIALSISWQAGLATAAAGAVLLAVLHTLVRTSLHAGRQQ
                     TALLKSLLSVMGGQLAAAKALKAMAREDHVDALLADQTKALRKALRRQVTSKEAMSAL
                     QEPLLAILVGIGFFFSLIILKLPLAEVLVMLLLLARTVSYLSKAQRAYQQVVVRESAY
                     WSLMENIAAARAEVEPRGGQQRVKLVEGITFDDVSFGHGERRAIVERGTFSVPAQQLT
                     VIVGPSGAGKTTLLDLIVGLLRPDGGRILVDGVPLGDLDLRQWRRQIGYVPQESVMVD
                     ESVAYNVALGEVDLSEDQIRAALRAADALDFVDALPEGLQTRVGEGGSLLSGGQRQRL
                     AIARALVHEPRLLILDEATSHLDPEAQAAVIETVRHLKGHLTILAVAHQDLLIQAADR
                     IYRLADAQVSEVRKQGDEIGLPVRG"
     gene            12473..13390
                     /locus_tag="PKV56_07995"
     CDS             12473..13390
                     /locus_tag="PKV56_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX67796.1"
                     /translation="MDLGLQFGRARRRLTYLIYRTLLRSVGFKRARMLGGWSGELQFR
                     LFWRQRHRLQRDVALILGRRSDDPYVVELLHEAYRVNNGAALEIMAMFDRRQDEHDLA
                     ACCELDGLEHLREAMAAGRGAILLATHSGNALLTTIRLAQAGWRISVVYRDARMMSEG
                     FFQDGLEQYGIQAILANGGIRAYGQMLTALKQGRIVFVMMDQGVKQAEDGLMQRFLGK
                     DLPMPAGPAQLARASRAPLLPLVTTAVSPVWRFEVRPQVALTGDTLESDVAFLVGVTE
                     QQILAYPKFWSWHHRRWGKSRLAPVSTRS"
     gene            13458..14519
                     /locus_tag="PKV56_08000"
     CDS             13458..14519
                     /locus_tag="PKV56_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOX67797.1"
                     /translation="MEPETNTAPIWVLLGAKHGDNQQLLAIAEALNLPFRTIALQFNR
                     LARQAPVLLGASRLSWHTDVPLEAPWPRALLAAGRKSVPAARWIRQQSGGRTRLIHVN
                     RPWAPLAWFDLIVSTPQYALPERPNVLANLLPFVRVPRDAMPLAALPIESAALPRPWT
                     GVLVGGKSRPFVLNDTGAADLAGTVNALVHKTGGSAWILGSPRTPSAAMQIVEQHISV
                     PARVVRWRPGEDLYGTLLGLVDSFVVTSDSASMLTDALLTGRPVTRFELPLEPDLKWR
                     IASAWRAAALQSPASVIARSFEAAVNIGLLSSVRDLGRLHRALNEAGLFSPDGRPREL
                     AESERRRTLDRIARVIEGA"
     gene            complement(14576..15469)
                     /locus_tag="PKV56_08005"
     CDS             complement(14576..15469)
                     /locus_tag="PKV56_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67798.1"
                     /translation="MRNAVFIHTNPKQIVGALVAQHALVRNSAAPERFDVRILQTPDF
                     PAFERFEGRRYLREGKQTVWRNDDLQSFTPLRFAVPEQMRYAGRAVITDPDVFALGDV
                     NELLDRDMQGAAVMARQMSDEFRRSLHYASSVMLMDCSRLEHWKWEADFEKLFRGERD
                     YRDWMWLLLEPAGSVGVLESQWNDFDRLESDTKMLHNTHRRTQPWKTGLPSDFTPRGT
                     TLKSRAGIWLRRFTGRGAGHYRQHPDPAQERLFFQLLGECLDNGSVSVSMLKDEIAHG
                     HIRTDALECIARARPASRLAA"
     gene            15781..16740
                     /locus_tag="PKV56_08010"
     CDS             15781..16740
                     /locus_tag="PKV56_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67799.1"
                     /translation="MKVLITGAGSSGSWAIRGEQLGQSIGATVQPLARSARGFDVVVI
                     VKRLHDRAILRSARWHGVPVIWDVVDAWPQGRGTDKVWTSEGSISWLRRSIRRIRPDG
                     VIAPTQKMELDLQSVLGKDALVTTLAHHAWPDKPINPIRQEVRSIGYQGSEKHLGLWS
                     GVLVEQARKRGWEFVINPPSLADVDIAVALRRDTGYAPKNWKSNVKLANAQASGTPII
                     LTRESGYLETASGSEFWADTPDELNAGLDWFSEHAHRLEASRRMLGTEPRLEDIGNDY
                     RQWLRSVCEAPHLFNRRHASRARRLCALASSLVVDRFRNRVGG"
     gene            complement(16762..17931)
                     /locus_tag="PKV56_08015"
     CDS             complement(16762..17931)
                     /locus_tag="PKV56_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67800.1"
                     /translation="MKVCFLYNAQLHQIPHSLPIALELAARHPDFDVDVAGASERHLD
                     FARRLADQYGFRSRLGYRLLDRPWIARLRSGLTGELAPKKKRTLTLNANYLSECDALV
                     TPERTSLDLRRSGLLPRRTRMVYTGHGAGDRAMAVAPEIHEFDFVFTFGEKLEKRRLE
                     AGLIRPGAYATGVYAKFDWVLATEGRRPRLFDNDRPTVLYSPHFDTGLSSWPALGWQV
                     LDFFARSTRYNLVFAPHVRLFEPPNYWKYFAFSRYAKCAHMLIDLGSERSIDMTYTLG
                     SDLYLGDVSSQVAEFMLRPRPCLFLDPRRTSWQGDPNYRFWTLGPVLSDIAGLDAALL
                     QAFETHPVYEPRQSAYFEETFGGAGRGEPSARRGADLMADFLRRPAPEHVPVAIA"
     gene            18027..19028
                     /locus_tag="PKV56_08020"
     CDS             18027..19028
                     /locus_tag="PKV56_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX67801.1"
                     /translation="MNVEQHISDRPTTPAPLAAVTGATGFIGRHLVATLAEAGWRTRL
                     LLRREPDEPQWRTVRPQVVAGSLGDEAALARLVDGADAVIHLAGLIKAARRADFFDVN
                     CESAARLARVVRRCAPRAHFVMVSSLAAREPLLSDYAASKRAGEAAVLDVLGSCATVL
                     RPPAVYGPGDRETLLFFQLARMRRVPLLGGPDARAALIHVGDLSRLIATLAASPPRGE
                     LLTAADGRPGGYRWDELLGAAARAVGNTEPRFVRAPQGVLGAVALVGDLARALGSASM
                     LNSQKLRELRHLDWSVSPAELAQAPGWTARFDIDNGFADAVAWYRSAGWLPRQDLTG"
     gene            complement(19025..19969)
                     /locus_tag="PKV56_08025"
     CDS             complement(19025..19969)
                     /locus_tag="PKV56_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67802.1"
                     /translation="MPPAGAELAAQIDAELRRPVPPPVTALARQLAGRAGAGTAAVLF
                     YGSALRDDALDGVLDFYVLIDRCRDWPGPRWVAFANWLLPPNVGYVEADAILPGLRAK
                     YAVMTLAQFRRGVSMRSVDTTLWARFSQPCACVHVRSETDWLAVRDAVAEAASTAARW
                     AALLGPSEGEPAAYWRGLYKRTYAAELRVERSSRGADLLTGVAARYERLLSAAWCAAG
                     LAFAVRADGSLAPLLDPGERARGERRWRLRQRLGQPLNLLRLLKAALTFDNAIDYVAW
                     KVERHSGYRIEPSPFQRRHPLLAAPLLYWQLRRRGVLR"
     gene            complement(19981..21105)
                     /locus_tag="PKV56_08030"
     CDS             complement(19981..21105)
                     /locus_tag="PKV56_08030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOX67803.1"
                     /translation="MSASAVPARRTAGVVVGDSPLRVWSLSSELRLRRQLERAGAVDD
                     ASHAPRTVLLRADWVYDDALVRGLCGADDDVALVASDGAVVALGLSAPEAGVEQMLAA
                     GRAPAAARKVTPAQIADGYNDALRKREPPYLLPLVAADLPAIERRIFAGSYKGVTDLV
                     TLYAWPRPARWVTRQCANAGITPNQVTSASLLLVFAAMALFWTGHYAWGLVAAWVMTF
                     LDTVDGKLARVTLTSSRWGNVFDHSIDLIHPPFWWWAWIVGLPAAGFVLPQADLALTV
                     IVAGYLLQRVEEGIFIACFGMDMHVWRRFDSRLRLITARRNPNLLLLSASLLAGRPDL
                     GILAVAWWTAVCFVIHGLRIAQAALVRRHGPLRSWLSAPA"
     gene            21203..22126
                     /locus_tag="PKV56_08035"
     CDS             21203..22126
                     /locus_tag="PKV56_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX67804.1"
                     /translation="MSDFAFAHISDLHLPFEPRLSLRQHFSKRQLSVWSWRRRGEVQS
                     DHILAALKRDLHDHAIDHVLLTGDITNFSLPGEFRHAAGWLSALAPADRISLVPGNHD
                     ALVPVPATEGIGQWDAWTRLDRGWPFAHRVRNVTLIGLNSALPTAPLLARGRLGPHQL
                     EQLERMLTDEGAAGRVRIVMLHHPAARGAIGWRKALADGDALRAVLARTGAELVLHGH
                     ARDARLDAIPGPREPIPCLCVPSSSALPNPKDQGARWHALRIIECDSDRRVEVTVRRW
                     SIDAGSFVTDIVYELCLPKQSPLAAGDNPAP"
     gene            22144..22602
                     /locus_tag="PKV56_08040"
     CDS             22144..22602
                     /locus_tag="PKV56_08040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOX67805.1"
                     /translation="MANATETKFGDPTTRLAQTDHWTVLLRPRQPTFGSLVLICREPV
                     RSFAEVSAPGYIELRDVVRRIEATLQDVVAYERINYLMLMMVDPDVHFHVIPRYAGSR
                     QFGDIEFRDAGWPGPPALDAAVALEPATSVALLNQLREAWLRSAGQDRAP"
     gene            22599..23807
                     /locus_tag="PKV56_08045"
     CDS             22599..23807
                     /locus_tag="PKV56_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOX67806.1"
                     /translation="MISWFTLDRLDRYSLRLITGPFAMALVALLLAQLLERLLRLFDL
                     AASAGAPPSSVLVMAATLVPHYLGLALPMAFTAAIFMAAAHMCDDNELDVMLVAGRSI
                     ARIAAPYFLLAMFLCVFSLYLYGQLQPLARYGYHVAVNRVLQTGWDARVEENRFIDIG
                     HGITFSADVIEGNGRDLRGVLMERRSAGNDEILTAARGHLVHTPDGLQLQLEDGQIVR
                     QRDVPGPSGTAISVSRFAHGLSGRDFAPEVEPLADRGSSVRERTLHELWRDMQPDKEA
                     VGRAKAAAEFHGRLARAMTFPLLPLLALPLGMASKRGRRTPGVIFAVLALLAIHHALQ
                     LGESLAESGRLPAAAAVWTPFAVFAALSLWIFRSSLAWPGDNPVLRAVMAIEAWFDRA
                     RTRTRPKTVA"
     gene            23804..24883
                     /locus_tag="PKV56_08050"
     CDS             23804..24883
                     /locus_tag="PKV56_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOX67807.1"
                     /translation="MNGRLASYLRRRVGMQILALLAVLTAMMQVLELLDVTTDILERG
                     QGVSGLLYYAALRTPAELALALPLAVLLGTMTALNAMSRGMEITAMRISGMSLMRLLR
                     DLLPVLLAVAAVQVVLLQAVLPRVEIELKQWWNATTPPEPATAPEKETPPLWAHTRDG
                     AVSIDTFSPDGRQLRDVRLYSQRAGQLTSRIRAATASWDGRAWQLEDVTELRIEASGV
                     RVIHEKMREWQSNLHPEEVLRLGLPRPYLSTMMLADVIAGSRVGSQPLSYYQTALYRS
                     FAAPLGVFIMLLLALPTAITPARATGGGAMLLALTLGLGFLLFDGIVAALGSSGKFPP
                     LATALTAPLLFASIGLWRLRACESL"
BASE COUNT         4300 a         8254 c         8109 g         4263 t
ORIGIN      
        1 gtcaaggtag tgggtacaca gctggaccac gtcttccggc gcgcccttga ccaccagctg
       61 gcgcatgccc gcgcgctcga cgaggacaga gacgcgcctg cgctcaaagt cgaatggcac
      121 ttcgtcgacc ttgacccagc ccgaaacgtc gatgtcttcg tgcgcgagga tggcgtcgtc
      181 cagcgggctc ttgaggccac tttcaaaata gctgttgagg taggccagtg cgagtacttg
      241 ggcactatcc ttgccgtcgg catccacatc gaccgtcagg cgaccgccgt caaccaggcg
      301 tccgaacagc agctcgtctg ccagagcacg acggatcgtg tcctggatca gacgctgcat
      361 gggacgcgcg cccatcagcg gatcgaagcc attcttcgcg aggtgcttgc gcagcgcatc
      421 ggtgaaggtg acatcgacct tcttctcggc caactggcct tccagttgca gcaggaactt
      481 gtcgacgacg cgaaggatga tttcctcatc cagtgcacgg aagctgacga tcgcatcgag
      541 ccgattgcgg aactcaggcg tgaacatccg cttcacgtcg cccatctcgt caccgcgctc
      601 gcgcggattc gtgaagccga tcgtggcctt gttcatcgtc tcggcacccg cgttggtggt
      661 catgacgatg atcacgttgc ggaaatcggc cttgcgcccg ttgttgtcgg tcagtgtgcc
      721 gtggtccatg acctgcagca gcacattgaa gacatccggg tgggctttct cgatctcatc
      781 gagcagcaac accgcatgcg gcttcttggt gatcgcctcg gtcagcagcc ccccctgatc
      841 gaatccgaca taccccggag gcgccccgat caggcgactg accgcatggc gctccatgta
      901 ctcgctcatg tcgaagcgga tcagctcgat gcccaggatg aaggccagtt gcttggcgac
      961 ctcggtcttg ccgacaccgg tggggccgct gaacaggaag ctgccgatcg gcttgtcggg
     1021 cttgcccagt ccgctgcgcg ccatcttgat ggcggcggcg agcgcgtcga tggccgggtc
     1081 ttgcccgaac accacgctct tcaagtcccg gtccaggctc ttcaacttgc cgcggtcgtc
     1141 gttgctgaca gaagcgggcg ggatgcgcgc gatcttggcg acgatctctt ccacctcgtt
     1201 gcgggtgatg gtcttcttct gcttgctctt gggcaggatg cgctgcgccg caccggcctc
     1261 gtcgatcaca tcgatcgcct tgtcgggcag gtggcggtca ttgatgaact tggccgacaa
     1321 ctcggccgcg gcctgcaatg cacccagcgc gtacttgacg ctgtggtgct cttcgaaccg
     1381 cgacttcagg cccttgagga tttcaatcgt ctcctcgacg gatggctcga cgacgtcaac
     1441 cttctggaaa cgacgcgaca gcgccgcgtc cttctcgaag atgccgcggt actcggtgaa
     1501 ggtggtggca ccgatgcact tcatcgcacc cgaactgagg gctggcttca agagattgct
     1561 ggcatccagc gtgccgccgg aagccgcacc ggcaccaatc agcgtgtgga tctcatcgat
     1621 gaaaagcacc gagttcggtt gatccttcaa gaccttcagg acacccttca ggcgctgctc
     1681 gaaatcgcca cggtacttgg tgccggccag caatgcgccc atgtccagcg aatagacggt
     1741 ggcgttggcc agcacctccg gaacatcgcc ttcggtgatg cgccacgcca ggccttcggc
     1801 gatggctgtc ttgccgacgc cggcttcgcc gaccagcagc ggattgttct tgcgtcgacg
     1861 gcacaggatc tggatgaccc gctcgacctc gtgttcgcgg ccgataagcg ggtcgatctt
     1921 gccgtcacga gccagctggt tgaggttctg ggtgaactgc tcgagcggcg agcccttgcc
     1981 gtcggagtca tccttctcgc tgtcgcctga actgccactg tcgctgggct tggcagcttc
     2041 gggcgggtcg gacttcttga tcccatgagc gatgaagttg acgacatcca ggcgcgtcac
     2101 tccctgctga tgcaggtagt acacggcatg ggaatccttc tcgccgaaga tcgcgaccag
     2161 cacgttggcg ccagtcactt ccttcttgcc gctgccggtg gactgcacat gcatgatcgc
     2221 gcgctggatc acgcgctgga agcccagcgt cggctgggta tcgacctcgt ccgttccacc
     2281 gaccgtcgga gtgttctcct tgatgaactg cgccagactc ttgcgcagat cgtcgacgtt
     2341 ggccgagcag gcacgcagca cttcggcagc agacgggttg tcgagcaacg ccatcagcag
     2401 gtgctccacg gtgatgaatt cgtggcgctg ctgacgggct tcgacgaacg ccatgtgcag
     2461 actgacttca agttcctgag cgatcatgcg gcctccataa tgcattgcaa agggtggcca
     2521 ccgcgccgtg cggcggccaa caccaactca accttcgtgg ctgcgacatc cttggaatag
     2581 gtgccgcaaa ctccacggcc gtcatggtga atcttcagca tgatttgggt tgcggcctct
     2641 aggtcacgat tgaaatactc ctgaagcacc atcacgacga attccatcgg cgtgtagtca
     2701 tcattgagca ggatgacctg atacatgcgc ggaggctcgg tcttctgaac ctgccgctcg
     2761 acaacaacgg caccctgccc gtcatcgcgc ggcgacccga cgacaggggg cttggatgct
     2821 ggtttgtcag tggacatgga ttcattctat cgagatgagt gattcaacaa gcactgcggg
     2881 catattgact ctggctatcg cgacataagg caaaaattga gcctcgcaag cccctcaatt
     2941 cagtccccga tacggtgcgc actggccaaa gtgaccactt gaatcaggtg cgcgcagtag
     3001 gtgtctcgcc gccttgacac cgcaaactgc gcagccggaa aattcgacag gcattctccg
     3061 gaggcgcagc atgtccacag gcaccgtgaa gtggttcaac gacgcaaagg gcttcggctt
     3121 catcgagccc gatggcggcg gagatgatgt tttcgcccat ttctcggcca tccagatgga
     3181 cgggttcagg acgctcaagc agggcggcaa ggtcacgttc gaactggtgc aaggcccgaa
     3241 aggccaactc gcgcagaaca tcgttccgga actcggcctg agcgagcatg tgccggagtc
     3301 acaaagagat cagaccagcc cggcgcaatc cctggctcat tgacccgatt gaggactcgc
     3361 ccagagatcc aatgagaaac ccgcctttcg gcgggtttcc tgttctatgc attccggttt
     3421 ttcgtgcccg ctgctgctgg cgcagattgg tgcgggccgc aggatggtca gggcttgcgt
     3481 gtctgggcat catgctttgc ccggtagacg tgccgactgg cgtgatcctg acgcttctcg
     3541 agcacgaccg cttccttgcg cgtgaccttg ccgtcggctt cggcgcgagc ctcggcacga
     3601 gtcaggcggg cttgctcacg gcccagtcga cgcgcctcgg gcttcgtcag cgcgccggag
     3661 gacacgccgt tctcgatgcg ttcttcctgg cggtcctggc ggttgtcgaa gcgctgctcg
     3721 gccgctggag aggctgcggt ttcagcgtag gcaacagaag tcgctgcggc aagcaacagc
     3781 gcgatcaggg tggtggaacg aacaggagac atggcgggtc cttgaggtga tggttgagtg
     3841 gtgcgcgtga cacaaccgca gtcacgtgcc ccttcaaccc gcggacgcac cacatcctta
     3901 cgcgccgatt catacccgac tcgattggtg ggagtcagcg taagaatcgg tcgactcacg
     3961 ggttcaacac acgcacatgc aggcgtggat gccagcctct tcccatcagg ggcgacagca
     4021 catgccaaag gagcgtcatg aagcggggat tgctgttctt gatggcgatg atctggccac
     4081 tctggggatt ggccgcggca cccgacgtgc ccgacccgga agcctgggtg cgactcagcc
     4141 cggccgagca ggctgcccgc cgagccgagt tgaagcagaa attgcagcag gccactccgc
     4201 aggagcgggc ggcttttcgc aatcgactgc gcgaacgtct cgaggaaatg ccccccgagc
     4261 aacgccaggc actggctgaa agaacccgcg aacgctggca aaagctcgac ccgcaggaga
     4321 agcagcgttt gatcaatgaa cgccgcgagc gagtgaaggc catgtcgccc gaggagcgca
     4381 ggcagctgat cgaacaacga cgggaaatcc tcggcaagct ttcgcccgaa gagcgcgcgc
     4441 tgttgcgcga gaaactgacc ggtccgtgag ccgctcgacc cgcgcatgga ctcgacccgg
     4501 atggatgccg tgtctcggct gaccggctgg ctggccggcg ccgcaggccg gatgcgcccc
     4561 ggtgagcctg cgcagggcgg tgactgggca ctgtggcgag aggcctgcgc gggcaacccg
     4621 cgaagtgcgc agcaactggt gcgagagctc accccgtcgg catatggcct ggcgttacag
     4681 atgctgcgcc gcgccgaaga cgccgaagac gcagtgcagg aggcctttct gcggctgtgg
     4741 cgcagccacc ccaccgacac cgaaggggcg aagctatcca cttacttcaa caccatcgtc
     4801 atcaaccggt gccgcagcct gctcgtggca cgccgggaag acgccaccga cccggatgcg
     4861 ctggccgacc tgcacgatgc gcggcaggtt tcgggggccc atggcggcca tcacgatgac
     4921 ccggccgaat cgggatgggc ggacacgggt tcggcgctgc agcgcggcct gatgcgactg
     4981 cctgttcggc aacgcatggc gatcgtgatg tgggcctatg ccgattcggg cgttgccgac
     5041 atagcccgca ccctggacat cgaccccaac gccgcgcacc agctcttgca tcgtgcaaag
     5101 caggcactgc gcattcacat cgaaggagcc gccccatgac tgaccgccct cccctcgagc
     5161 accgggatcg gtccgatccc gccgacgacc aattgcggtc gaggctgcgc gagttgcggc
     5221 ctggagccag cgcgatcgac gcgctcggtg cccgcgtgct ggtccagtgg agcgaacaac
     5281 atcaccatgc cggtcgcacg accctgcatc tcgggggcac gatgggaacg gccgagttcg
     5341 gcctgggccg ctcgcatcgg cgactccggg ccagcctcgc ctccggtctg gttgtctgcg
     5401 ccatcgtcgc cgcagtcatg tgggcgcagc ggcccgaccc gacgctggac gagctgatac
     5461 aggccgatgt gctgtcgcag atggcgatcg gcgagatgtg acggccgccg ctgcgggtta
     5521 ccacccccgc tgagaacccc acctcagacg ttgcccggcg cagacaggaa ccgaaagccg
     5581 gagcatcatc tccaacccgg ccggacgaca ccgcaagtcg ccgctgccgc gcctgaaagg
     5641 acccgatcat gtctctccca gcttctgctt ccccgaccct gcgcagccgc cgcagggtgc
     5701 tcgccagcgc actcgccaca gggttggcgc cggtgctcgc ccgcgccgcc tccgaagtcg
     5761 ccgacaacga gaacaacgag cgctggcaga tgcttcgcaa gtcactgttt cagaaccgca
     5821 cgatctccga agactccgcc gcgatcatcg aactggacac accggcgcgc gctgccgatg
     5881 ctgccgtggt gccgatcgcg ttccgcaccc gcttcgttca gaccgccggg acctatatca
     5941 agaaggtgca tctggtcatc gaccgcaatc cgtcccccat gggcgccacc ttcacgttca
     6001 ctccggaaag tggccgtgcc gagatcgaga cccgcgttcg catcgaggag tacacccacg
     6061 tgcgcgccat cgccgaaatg aacgacgggc gcttgttcat gcacacgcgg ttcgtcaagg
     6121 cctcgggcgg ctgctcagcc ccggcgggca aggacctgca ggccgcactg gccaacgcag
     6181 gccggatgaa gctccgcgtc gagggtgacg tcgtgctcgg tcagcccacg ttggcgcagt
     6241 tgtccatcag tcacccgaac gtgtcgggtc tggccatcga tcaggtgtcg cgcctcgccc
     6301 caccccctca ctatgtccgc agcatcgagg tggcctatgc cggcaagccg gtgctgagtg
     6361 ccgacctcga tttcacgatc agcgagaacc cgaacgttcg cttcttcttc gtgccgaagg
     6421 aggccggtca gctgaccgtg aatgtcatcg acagcaacga cgcgaagttc gagaaatcgc
     6481 tggccgtggc gccgggccaa cgcagcgatg cctgagcgcg gcgagcccgc atcgaccgaa
     6541 ccgcccggca cttcaccgga cgacttgatt ccggtcgctg gtggcgggct gctgcaccga
     6601 cgcgcactgc tgcaccgagg cctggttttc gccagtgccg ctccggctgc gatgctgacg
     6661 accggggcaa gtgccgccag tccgcctcac gacccagtag tcgccgccgc ctcgggtgag
     6721 ttcccagcgt ggatgcgcac accgggaacc ccgttcagca cctatggcca accttccagg
     6781 cacgaaagcc aggtcgtgcg gcgcatcggg gccaacaggc tcgcgccggg caacggcgtg
     6841 tcgtggacgc cgctggaaga cctcgagggc ttcatcactc cgaccggcct gcatttcgag
     6901 cgccaccaca acggcgtgcc cgagatcgat ccggcacgcc acacgctgac gatccacggc
     6961 cgcgtgaaga gtccgctggc gttcagcgtc gctgatctgc tgcgctaccc gatgcaatcg
     7021 caactggtct tcatcgagtg cggcggcaac agcaacgcag gctggcacaa cgtaccgata
     7081 cagcgtccgg tcggctcctt ccacggcatg gtttcgtgca gcgaatggac cggggttccg
     7141 ctgtcgctgc ttcttgatga agccggcctc gactcgtctg cgacctgggc cgtggccgaa
     7201 ggcgccgatg cgatcgccat gaatgtcagt ctgcctgtgg acaagctgat ggatgactgc
     7261 ctcgtggcgc tctatcagaa cggggagagg ttgcgccccg aacatggcta cccgctgcgg
     7321 ctgatcgtgc ccggatggga aggcgtgctg aacgtcaagt ggctgcatcg cctggagttg
     7381 acggaccagc ccgtgatggc gcgaaacgag accgccaagt acaccgaact gcaaccggac
     7441 ggcaaggcgc gacagttcac tttcgtgatg gaggccaagt cgctgatcac ctcgccatcc
     7501 cacggtcaac acctgccgag cgcagggtat taccagatcg gcggtctggc atggagcggg
     7561 cggggccgca ttcgccgggt ggaggtgtcg gccgatggcg gacggacctg ggccgatgcc
     7621 gcgctgcagg atccggtgct gccgcgctgc ctgacgcgct tccgcttgcc ctggcactgg
     7681 tcaggccggc cggcgatcct gaaaagccgc gccaccgatg aaagtggcta tgtgcagcca
     7741 gaacgccagg cgctgatctc gcagcgaggg tcgaatggct atttccactg caacgcgatc
     7801 gtcagctggg ctgtcgacga agacggtgac gtgactcatg tctatgcctg agcgcccgca
     7861 cttccgttct ctgatcggcg ttctcatcgc tgcgctcgca agcatccagg gaggcggagc
     7921 ctgcgctgcc gaggcgcccg gcgcccccag gctgggccac aggctgactc cggagcaggc
     7981 gcagcgacag tcgctgaacg tgtttccaga cgggcgaggc ctgccggccg gtcggggcac
     8041 ggcggcagag ggccgaccgc tgttccagca gaaatgcgct gcgtgccacg gccaggccgg
     8101 tcgaggcgcc agcgctgaag agctggccgg tggcgaggcg ccgctcacca gccagtcacc
     8161 cgacaagacg atcgggttgt actggccgta cgcaacgacg atcttcgatt tcacgcgacg
     8221 cgcgatgccg ctgagcgcgc ccggctcgct gagcgacgac gagatctatg cgctgacagc
     8281 ctacctgctg ttcgagaaca gactgatcgg cgagcgcgaa gagatgaacc cggtcacgct
     8341 gccacgcgtg cacatgccga accgggacgg gttcatcggc accgatgcgc aagcgcgttg
     8401 acgaatcaag caccagtacc agtgcgctgt tgcgggggca ccggctgcgg cccgtgccca
     8461 cccgaaatga catgcacgcc acgccagagc tgggttcggc gcatgggaaa gtcagcctga
     8521 tcaggccggg ccgcgcagca ggtatgcttg gacgttttgc ctccctgcaa gctccaacgt
     8581 gacctcaacc gcaacacctg acgagtccgc tacggaatcc tcgcccgccg tcgagaacga
     8641 caccgtcggg caggacagcg gccgcgccct gatcagcgat ctgtttcgtc gttctcccgc
     8701 aatttactgg gtcgacctgc tgttgagtgc cggcacggcc tgggcgctga ccgcggtcta
     8761 tttcaccgcc ccggcctgga gcgcgctgca ggtcgccgcg ttcgtggtcg cctcattcct
     8821 gttctttcgg gcgggcacgt tcatccacga gatcgttcac atgccaggcg ggcagatgga
     8881 aggtttcaag cgcacctgga acctgctgct cggcgtgccc ttcctgatgc catgggtcat
     8941 gtatcgcaac cacatcgagc accacaacca ccggcatttc ggcacaccgg cagatggcga
     9001 gtacctgccg ctgggttctt cgccacgggt cgagaccctc aagtacctgg ctcaggcacc
     9061 cctgcttccg ttgttcatga tgatccggtt cggcatcctg ggtccggctt cgtggctgca
     9121 ccccaggctg cgtgaatggg tgctcacccg cgcgtcggcg gcggtctcca acccctacta
     9181 caacaagcga ttcccgaagc gcgatgaagc gcatctgcgg gtggtcgagg tgctgtgcct
     9241 catctacttg atcgcgatcg cggcgctgct ctggctcgga tccatcagcc cggtgcatct
     9301 gttcatgggc tacctgctcc tggcctgcac gctgggcctg aactgggtgc gcaacctggc
     9361 cgcgcaccac tatgtcaatg gcggtggacg catgacgatg gcggagcaac tcgccgattc
     9421 gatcaacgtc accggacaga cctggctcac catcgcgatg ttcccggtgg gtctgcgcta
     9481 ccacgccctc catcacatgt tcccgggcgt cccctaccac cacatgggcg aggcccatcg
     9541 acgcctgatg caggggttgc cgcccgactc ggtctatcgg ggctgcaacc gcgacagctt
     9601 cttctcggcc gtgcgcgagc tgtggcgatc ggccagccac acgccgccgg agcagtctgc
     9661 gatgcgggcg tgggtcgcgg gctccggtcg cacttgaacg tgcgcgccgc cgggctgacc
     9721 gccccggatc tgacggtcgc ggcagacgct ggggcaccgc cctgcctgat cgtcaatccg
     9781 cgcagtttca gtgcttcgcg cgggttggcg cagcaggcat cggctctggc gcggacacac
     9841 ggcgccgaga tcgtcaatgt cgacgggcct gccgccttgc gcgccgccgt cgagtccatc
     9901 ctggcgaggc gccagcgtac ggtcatggtg ctggccggcg atggcaccgt gtgtgccatc
     9961 gtcgatcagc tgtccaatct ggcgccgggg gcctggaccc ccgacctgtt gatcctgccc
    10021 ggtggtcgca ccaacttgac ggcggtcgat ctgggcacag gaagccgagc cctgccgaca
    10081 ttgaagcggg tgctgtcgat gggccgggat cagcgcggga acgtcgctgt cgaggaacgc
    10141 tgcgcgttgc gcatcgagca gtcgccagct cctgctcgcc acggattctt cttctcggct
    10201 gcactgatcg acagcgcgac acgccagtgc caccagcggc gcatcgaccg caacgagcac
    10261 ggcccattca ccacattgag ctacttgctg aagctcggag tgctcgccct gttcggccga
    10321 accgggctgg tgtgcccgac cctgagcgtc gatgcgcaag ggtgcggcac ccagcaggga
    10381 cccgtctgcc tgctgatggc cagcacgctg agccaccgca aggggctctt cgatccgtat
    10441 gctgcgcgcg gccagggcga cgtgcggatg atgtccgtca gccgcgacgc tccggggatc
    10501 tggcgctcgc tgcctcggct gctgaccggg cggtactcga aggcaatgaa tgccgacaac
    10561 ggctacgtca gcggacgctg cacacaggtg gacatctccg ggctgagcgg ctacatgctc
    10621 gacggcgagc ctttcgacgc ggatcccgac cggcccgtca cgatcacggc gggtcgccgc
    10681 cttcgcttcc tgaagccatg agcgcctccg acaagcaggg cctcacgtcc gggcgcagcc
    10741 gcgcgttccc gatgatccgc gccttcgtcc tcgcctaccc cgggcgcagc gcgacagcgc
    10801 tcggtgcggt cttcctggcg ggtctgatgg acgggctggg cctgtcgatg ctgctgtcca
    10861 tgctgtcctt cgccacgcgc accgacaagc atgagccttc gatgcccgag cagttcgcgt
    10921 tgcgcgtggc cgagttcgtc ggcctgcaac cgagtgcgcc ggtgctgctg atgctcgcga
    10981 cggtgctgat agccatgaag gccgtgctgt cgctggccgc caatcggcag gtgggctaca
    11041 ccgttgccaa catcgccacc gatctgcggc tgtcattgat tcgctcggtg ctgtctgcac
    11101 gctggtctca ctaccttcag cagtcggtcg gtcgcctgtc caacgccgtc tccaccgaag
    11161 ctcagcgtgc ctccgaagcc ttccagtgca gcgccgagat ggccgcgatg ctgctgaatt
    11221 cgatgatcta cctggtcatc gcgctgtcga tttcctggca ggccgggctg gccaccgcgg
    11281 cggccggcgc tgtgctgctc gcggttctgc acacgctggt gcgcacgtcc ttgcacgccg
    11341 ggcggcagca gacggcgctg ctgaagtcgc tgctgtcggt gatgggcgga caactggctg
    11401 cggcgaaggc gctcaaggca atggcccgcg aagaccatgt ggatgcactt cttgccgacc
    11461 agaccaaggc gcttcggaag gcgctgcggc gccaggtcac cagcaaggag gcgatgagcg
    11521 cccttcagga gccgctgctg gcgatcctgg tcggcatcgg tttcttcttc agcctgatca
    11581 tcctgaagct gccgctggca gaagtgctgg tcatgctgct gctgctcgcg cgcacggtga
    11641 gttacctgtc gaaggcgcag cgcgcctacc agcaggtcgt cgtgcgcgag agcgcctact
    11701 ggtccctgat ggagaacatt gcagccgccc gcgccgaggt cgagccgcgc ggcgggcagc
    11761 agcgcgtgaa gctcgtcgag ggcatcacct tcgatgatgt gagcttcggc catggcgagc
    11821 gtcgtgccat cgtcgagcgc ggcacgttca gcgtgccggc gcagcagctg accgtgatcg
    11881 tcggtccttc gggcgcgggc aagacgacgc tgctggacct gatcgtgggg ctgctgcggc
    11941 ccgatggcgg aaggatcctc gtggacggcg tgccgctcgg cgatctcgac ctgcgacagt
    12001 ggcgtcggca gatcggctat gtgccgcagg agtcggtgat ggtcgatgag tccgtcgcct
    12061 acaacgtggc cctgggcgag gtcgacctca gcgaagatca gatccgtgcg gccctgcgcg
    12121 cagccgatgc gctcgatttc gtcgatgccc tgcccgaggg gctgcagaca cgcgtcggcg
    12181 aaggtggttc gctcctgtcc ggcgggcagc ggcagcggct ggcgattgcg agggcgctgg
    12241 tgcacgaacc gcgactgctg atcctcgacg aggccaccag ccacctcgac ccggaggccc
    12301 aggccgccgt gatagagacc gtgcgtcacc tcaagggcca cctgacgatc ctcgccgtcg
    12361 cgcaccagga tctgttgatc caggccgctg accggatcta ccgtctcgcc gacgcccagg
    12421 tcagcgaagt gcgcaagcag ggcgacgaaa tcggacttcc ggtgcgcggt tgatggatct
    12481 gggcctgcaa ttcgggcgcg cgcggcgccg cctgacctat ctgatctatc gcacgctgct
    12541 gcgctcggtc ggcttcaagc gggcacgcat gctcggcggc tggtctggcg agctgcagtt
    12601 ccgcctgttc tggcgccagc gccaccgcct gcagcgcgac gttgcgctga ttcttggacg
    12661 ccggtccgac gatccgtacg tggtcgagct gctgcatgag gcctaccgcg tgaacaacgg
    12721 ggcggcgctg gaaatcatgg ccatgttcga tcgccgccag gacgagcacg atctggcggc
    12781 atgctgcgaa ctcgatggtc tggagcattt gcgcgaggcg atggccgcag gccgcggggc
    12841 catcttgctg gccacccact caggcaacgc actgctgacg acgatccgcc tcgcacaggc
    12901 cggctggcgg atcagcgtcg tctaccgcga tgcgaggatg atgtcggaag gcttctttca
    12961 ggacggactg gagcagtacg gcatccaggc gatcctggcg aacggtggca tccgcgccta
    13021 cgggcagatg ctcacggcgc tcaagcaggg ccgcatcgtc ttcgtgatga tggaccaggg
    13081 cgtcaagcaa gccgaggacg gcctgatgca gcgctttctc ggcaaggacc tgccgatgcc
    13141 tgcaggtccg gctcagctgg ctcgcgcatc gcgagctccg ctgctgccgc tggtcacgac
    13201 cgcggtctct ccggtgtggc gcttcgaggt tcgaccccag gtggcactga caggcgatac
    13261 gctcgaatcc gacgttgctt tcctggtcgg cgtgaccgaa cagcagatcc tggcgtaccc
    13321 gaagttctgg agctggcatc accgtcgctg gggcaagtcc cgcctggcgc ccgtatcgac
    13381 ccgttcctga cccgaccgac agcttaagtt tcacaacagg tctctaggat ggaatcgcct
    13441 gaaccacacg gcgacccatg gaacccgaaa cgaacaccgc cccgatctgg gtcttgctgg
    13501 gcgccaagca cggcgacaac cagcaactgc tcgccatcgc cgaagcgctg aatctgccct
    13561 ttcggaccat tgcgctgcaa ttcaaccggc tggccagaca ggcgcccgtg cttctcggcg
    13621 ccagtcggct gagctggcac accgacgtgc cgctggaagc cccctggccg cgggccctgc
    13681 tggccgccgg acgcaagagc gtgccggctg cgcgatggat acgccagcaa tccggcgggc
    13741 gaacacgact gatccacgtg aaccgtccct gggcaccgct cgcatggttc gatctgatcg
    13801 tgagcacccc acagtacgcg ctgcccgagc gtcccaacgt cctggccaac ctgctgccgt
    13861 tcgtgcgcgt gcccagagac gcgatgccgc tcgcagcgct gccgatcgaa tcggctgccc
    13921 tgcctcgtcc ctggacgggc gtgctcgtgg gcggcaagag ccggcccttc gtgctgaacg
    13981 acacgggcgc cgccgatctg gccgggacgg tgaacgcgct cgtgcacaag acgggtggca
    14041 gcgcctggat cctgggcagc cccaggactc catcggcagc catgcagatc gtcgaacagc
    14101 acatctcggt accagcccgt gtcgtgcgct ggcgtccagg agaggacctc tacggcacgc
    14161 tgctgggcct ggtcgactcc ttcgtcgtga cctcggacag cgcatcgatg ctgacggatg
    14221 ccctgttgac cggtcgcccg gtgacacggt tcgaactgcc gctggaaccc gacctgaaat
    14281 ggcgcatcgc ttcggcatgg cgcgcggccg ccctgcagtc accggcttcg gtcatcgcgc
    14341 gcagcttcga ggctgcggtc aacatcgggc tgctttcgtc ggtgcgtgat ctgggccgac
    14401 tgcatcgcgc cctgaacgag gcggggctgt tcagccctga cggacggcca cgcgaactgg
    14461 ctgaaagtga acgaagaaga acgctggacc ggatagcccg ggtgatcgaa ggtgcctgat
    14521 cggtggccgg agaggcctac accgatccag cacggtccgt cagggtcgcg gggcctcagg
    14581 ccgcaaggcg cgacgccgga cgagcccgtg caatgcactc gagtgcatcg gttcggatgt
    14641 gaccgtgcgc gatctcgtcc ttcagcatgg aaacgctgac cgagccgttg tccaggcact
    14701 cgccgagcaa ctggaagaac aaccgctcct gtgccggatc ggggtgctgc cggtagtgtc
    14761 ctgcgccacg cccggtgaat ctgcgcagcc agataccagc ccgcgacttc agggtggtgc
    14821 cgcgcggcgt gaagtccgat ggcagcccgg tcttccaggg ctgagttcgg cgatgcgtgt
    14881 tgtgcagcat cttggtgtca ctttcgagcc ggtcgaaatc attccactgg gattccagca
    14941 ccccgacgct gcccgcaggt tcaagcaaca gccacatcca gtcgcgatag tcgcgctcgc
    15001 cacgaaacag cttctcgaag tcggcttccc atttccagtg ttcgagacgc gagcaatcca
    15061 tcagcatcac gctcgatgcg tagtgcaacg agcggcggaa ctcgtcgctc atctgccgcg
    15121 ccatcaccgc cgcgccctgc atgtctcggt cgagcagttc gttgacgtcg cccagagcaa
    15181 agacgtccgg gtcggtgatc accgccctgc ctgcatatct catctgctcg ggaacggcaa
    15241 accgaagtgg cgtgaacgac tgaaggtcat cgtttcgcca gaccgtctgc ttgccctcgc
    15301 gaaggtaacg gcgaccttcg aagcgctcga aagccgggaa atccggggtc tgcaggatgc
    15361 gcacgtcgaa acgctcgggt gccgcactgt tgcgcaccag cgcatgctgc gcgaccagcg
    15421 ctccgacgat ctgcttggga ttggtgtgga tgaagacagc gttgcgcatg tcggaatcgg
    15481 tgactcgaga tcgaggggct ccgggaaacc gccgtcacgc ggtcagccgg tgcgaggtcg
    15541 cttcattgac ggcaagacat tgccaaccat gctaccgcga cgccgacttc gattcgcgca
    15601 tggcaacacg agccgttacg acatcggtgg tcggggtggg ctaccctctg gaacggacgc
    15661 tccggttgcg atgcacgatc ctgtgaattg cgtccgatcg ctgcccgtga acgaggatct
    15721 gccactccag tgcgtgggat ccgacaggac aaccaaggtc tcgcagatga aggaacgtct
    15781 ttgaaggtgc tcatcaccgg tgccggatca tccggaagct gggccatccg gggcgagcaa
    15841 ctgggtcagt cgatcggcgc gacggttcaa ccactggccc gttcggcacg gggcttcgac
    15901 gtcgtcgtca tcgtcaagcg gctgcacgac agggccatcc tcagatcggc gcgctggcac
    15961 ggcgtgcccg tcatctggga cgtcgtggac gcttggcccc agggacgggg cacggacaag
    16021 gtctggacat ccgagggatc gatatcgtgg ctgcgtcgat cgattcgaag gatcaggccc
    16081 gatggcgtca tcgcacccac tcagaaaatg gaactcgacc tgcagtcggt gctcggaaag
    16141 gacgcgctgg tcacgaccct ggctcaccat gcctggccgg acaagccgat caatccgatc
    16201 agacaggaag tcagatccat tggctaccag ggcagcgaga agcacctggg tctgtggtcc
    16261 ggggtgttgg tcgaacaggc gcgcaagcgc gggtgggagt tcgtcatcaa cccaccatcg
    16321 ctggcagacg tcgacattgc agtggcgctt cgccgggata cgggctatgc gcccaagaac
    16381 tggaaaagca atgtcaagct ggccaacgcc caggccagcg gcacgccgat catcctcact
    16441 cgagagtcag gctacctgga gacagcgtcg ggcagcgagt tctgggccga cacgccggat
    16501 gaattgaacg ccggactcga ctggttttca gagcatgcgc atcgcctcga ggccagccgc
    16561 cggatgctgg gcacagaacc ccgactggaa gacatcggca acgactaccg gcaatggctg
    16621 cgaagcgtct gcgaagcgcc ccacctgttc aacaggcgcc atgcttctcg ggcgcgtcgc
    16681 ctctgcgcac tggccagcag cttggtggtg gaccggttcc ggaaccgagt cggcggctga
    16741 tatgcgccgg caaacttcag gtcacgcgat ggctaccgga acatgctcgg gcgccgggcg
    16801 ccgcaggaaa tcagccatga ggtcggcccc acgacgggca ctcggctcgc cgcgcccggc
    16861 tcccccgaag gtttcctcga aatacgcgct ctgcctgggc tcgtagacgg ggtgcgtctc
    16921 gaaggcctgg agcagcgcgg cgtcgagtcc agcgatgtcg ctcagcaccg gtccgagcgt
    16981 ccagaaccgg tagttcgggt cgccctgcca ggaagtccgc cgcggatcga ggaacaggca
    17041 ggggcgcggg cgcagcatga actccgcgac ctgactgctg acatcgccca ggtagaggtc
    17101 ggatcccagc gtgtacgtca tgtcgatgct gcgctcgctg cccagatcga tcagcatgtg
    17161 ggcgcatttc gcataacggc tgaacgcgaa atacttccag tagttcggtg gttcgaacag
    17221 acgcacgtgc ggagcgaaca caaggttgta gcgcgtgctc ctcgcaaaga aatcgagcac
    17281 ctgccagccg agcgcaggcc acgatgacaa cccggtatcg aaatgcgggc tgtagagcac
    17341 ggtaggtcga tcgttgtcaa agaggcgcgg tcggcggcct tcggttgcca gcacccaatc
    17401 gaacttggca tacacaccag tcgcgtaggc gccggggcgg atcagaccgg cctcgagccg
    17461 gcgcttctcg agcttctcgc caaaggtgaa gacgaaatcg aattcatgga tctccggcgc
    17521 cacagccatc gcgcggtctc cagcaccgtg tcccgtgtag accattcgcg tgcgccgggg
    17581 cagcagtccg ctgcggcgca gatccagtga ggtgcgctcc ggcgtcacca gggcatcaca
    17641 ttcagacagg tagttcgcat tgagcgtcag cgtccgcttc ttcttcggcg ccagctcgcc
    17701 cgtgagaccc gacctcaggc gcgcaatcca gggacgatcg agcaggcgat acccgagtcg
    17761 ggagcgaaac ccgtattgat cggcaaggcg ccgcgcaaag tccagatgtc gctcgctcgc
    17821 ccccgccacg tcgacgtcga aatcgggatg cctggcagcc agctccagcg cgatcggcag
    17881 cgaatgcggg atctgatgga gctgcgcgtt gtacagaaag cagactttca tcaggtcgtc
    17941 agggtcgcca agtgcactcg aaagatactc catgaaggct gcccgcgccg cagggtgcag
    18001 acctcggcat actttcccgc acacaaatga acgtcgagca gcacatttcc gaccgaccaa
    18061 cgacccctgc cccgctggcc gcagtgaccg gcgccaccgg cttcatcggt cggcacctcg
    18121 tcgccacgct ggccgaggct ggttggcgca cccgcctgct gttgcggcgc gaaccggacg
    18181 agccgcaatg gcgcaccgtg cgtccgcagg tggtggccgg ctcgctgggc gatgaagcag
    18241 cgttggcaag gctggtcgat ggcgcggatg cggtgatcca cctggccggt ctcatcaagg
    18301 cggcgagacg agccgacttc ttcgatgtca attgcgaatc tgcggccaga ctggctcgcg
    18361 tcgtgcggcg atgcgcaccg cgggcgcact tcgtgatggt gtccagcctc gcagcgcgcg
    18421 aaccgctgct gtcggactac gccgcgagca agcgtgcggg tgaggccgcg gtgctggatg
    18481 tgctggggtc gtgcgcgacg gtgctgcgtc caccagcggt ctacggcccg ggggaccgtg
    18541 aaaccctgct cttcttccag ctggcccgca tgcgccgcgt gcccttgctg ggtgggccgg
    18601 atgcccgtgc ggccctgatt catgtgggag acctgtcacg gctcatcgcg acgctcgcag
    18661 ccagcccgcc gcgcggtgag ttgctgacgg ccgcggacgg cagacccggg ggctaccgct
    18721 gggacgaatt gctcggcgcc gccgcgcgcg cagtaggcaa taccgagccg cgcttcgtgc
    18781 gggcgccgca aggcgtgctc ggcgcggtcg cactggtcgg cgatctcgcg cgcgcactgg
    18841 gttcggccag catgctcaat tcgcagaagc tgcgcgaact gcgtcatctg gactggtcgg
    18901 tgtcgccggc ggagctggca caagcgccag gctggaccgc ccgcttcgac atagacaacg
    18961 ggttcgccga tgccgtggcg tggtaccggt ctgccggctg gctgcctcgt caggacctca
    19021 ccggctagcg caacacaccc cggcgccgca gctgccagta aagcagcggt gcggccagca
    19081 acggatggcg ccgctggaat gggctcggct cgatacggta accactgtgc cgttcgacct
    19141 tccaggcgac atagtcgatc gcgttgtcga aggtcaatgc cgccttgagc aggcgcagca
    19201 gattgagcgg ttgaccgagc cgttgccgca ggcgccaccg tcgctcgccg cgggcacgtt
    19261 caccaggatc gagcaacgga gccagactgc cgtcagcgcg caccgcaaag gccagtccgg
    19321 cggcgcacca ggctgcagac agcagcctct cgtagcgggc cgcgacgccg gtcagcagat
    19381 cggctccgcg gctgctgcgc tccacgcgca actcggccgc atacgtgcgc ttgtagagcc
    19441 cgcgccagta ggctgccggc tcgccctcgg agggccccag cagcgccgcc caccgcgcag
    19501 ccgtggaggc ggcctcggcc accgcatcgc gcacagccag ccagtcggtc tcgctgcgaa
    19561 catggacgca ggcgcagggc tgcgagaaac gcgcccacag cgtcgtgtcg acggaccgca
    19621 tcgatacgcc gcgccgaaac tgcgccagcg tcatcaccgc gtacttggcg cgcaaaccgg
    19681 gaaggatggc gtcagcctcc acgtacccga cattgggcgg cagcagccag ttggcgaacg
    19741 ccacccagcg cgggccgggc cagtcgcggc atcggtcgat cagcacgtag aagtccagga
    19801 cgccgtcgag cgcgtcgtcg cgcagcgccg agccgtagaa cagcaccgcg gccgtgcccg
    19861 caccggcgcg cccggcgagc tgtcgggcca gcgccgtcac cggcggcggc acgggacgac
    19921 gcagctcggc gtctatctgc gcagccagct cggcacccgc cggcggcatc cgatcctgcg
    19981 tcacgccgga gcagaaagcc aggaacgaag cggcccgtgg cgacgcacca gcgccgcctg
    20041 agcgatgcgc agaccgtgga tcacgaaaca cacggccgtc caccacgcca cagcaaggat
    20101 gccgaggtcg ggacgtcctg cgagcagcga ggcactcaac agcagcaggt tcggattgcg
    20161 ccgcgcggtg atcaggcgca agcgactgtc gaatcgccgc cagacatgca tgtccatgcc
    20221 gaagcaggcg atgaagatgc cttcctcgac tcgttgcagc aggtagccgg ccacgatcac
    20281 ggtcagcgcg agatcggcct gaggcaggac aaagccggcc gcgggcaggc cgacgatcca
    20341 ggcccaccac cagaacggcg ggtggatcag gtcgatcgag tggtcgaaga cgttgcccca
    20401 gcgcgatgag gtcagcgtca cgcgagcaag tttgccgtcg acggtgtcga ggaaggtcat
    20461 gacccaggcc gcgaccagcc cccacgcata gtgcccggtc cagaacagcg ccatcgccgc
    20521 aaacacgagc agcaggctgg ccgaggtgac ctggttcggc gtgatgccgg cgttcgcgca
    20581 ctggcgcgtg acccagcggg ccgggcgcgg ccacgcgtac agcgtgacga ggtcggtcac
    20641 gcccttgtag gagccggcga agatccgacg ctcgatcgcg ggcagatcgg cagcgaccag
    20701 cggcaacaga tagggcggtt cccgctttct caaggcatcg ttgtagccat cggcgatctg
    20761 ggcgggcgtg accttgcgcg ccgcagcggg cgcacgtcct gccgccagca tttgctcgac
    20821 gcctgcttca ggagcggaca aacccagcgc aacgaccgca ccatcggacg caaccaaagc
    20881 cacatcgtcg tcggcaccgc acagaccgcg caccagcgcg tcgtcgtaca cccagtccgc
    20941 gcgcaacagg acggttcgcg gcgcatggga cgcgtcatcg accgcaccgg ctcgttccaa
    21001 ttgccggcgc agccgcaact cggagctcag gctccagact cgcagaggcg agtcgccaac
    21061 caccacgcca gccgtgcggc gggcgggaac tgctgaggcg ctcatcggcc ccacccatcg
    21121 cctgacggcg agaattcttt tggcatcatc aacgggtgaa cacccaaggc gtgaaagtcg
    21181 gaaccgcagc agcgcattat cgatgagcga tttcgcgttc gcgcacatct cggacctgca
    21241 cctgcccttc gagccccggc tttcgctgcg gcagcatttc tcgaagcggc agctcagcgt
    21301 gtggtcgtgg cgtcggcgag gcgaagtgca aagcgaccac atcctcgcgg ccctcaagag
    21361 ggatcttcac gaccacgcga tagaccatgt gctgctgact ggcgacatca ccaacttctc
    21421 tctgccggga gagttcaggc acgcggcggg ctggctctcg gcacttgcac cggccgatcg
    21481 catcagcctg gtccccggca atcacgatgc gctggtgccg gtgcctgcaa ccgagggcat
    21541 cggccagtgg gacgcatgga cacggttgga tcgaggctgg ccgttcgcgc atcgggtgcg
    21601 caacgtgacg ctgatcggcc tcaattcggc actgccgacc gcgccgctgc tggcgcgagg
    21661 ccggctcggc ccgcaccagc tcgagcagct tgaacgcatg ctcaccgacg aaggggctgc
    21721 cgggcgggtg cgcatcgtga tgctccacca cccggcagcg cggggcgcca taggctggcg
    21781 caaggcgctg gccgatggcg atgcgctgcg tgctgtgctg gctcgcacgg gcgcggagct
    21841 cgtgctgcac ggacacgccc gcgatgcacg cctggatgcg attcccgggc cacgcgagcc
    21901 gatcccctgc ctgtgcgtgc cctcgtcgtc ggcccttccg aatccgaagg accagggcgc
    21961 gcgctggcat gcattgcgca tcatcgaatg cgacagtgat cgccgcgtcg aggtgacggt
    22021 gcggcgctgg tcgatcgatg cggggtcgtt cgtcaccgac atcgtctatg agctgtgtct
    22081 gccgaagcag agcccgctcg ctgcaggcga taatccggca ccctgagaca gacgggtccg
    22141 accatggcca atgcgacaga gaccaagttc ggagatccca cgacgcgcct cgcgcagacg
    22201 gatcactgga ccgtgctgct tcggcccaga caacccacat tcggatcgct ggtcctgatc
    22261 tgccgggaac cggtgcgttc gttcgccgaa gtcagcgctc cgggctacat cgaattgcgc
    22321 gatgtcgtgc gccgcatcga ggccacgctg caagacgtgg tcgcctatga acggatcaac
    22381 tacctgatgc tgatgatggt cgatccggat gtgcatttcc atgtgatccc acgctacgcg
    22441 ggatcgcgcc aattcggcga catcgaattt cgcgatgccg gctggccagg cccgccggca
    22501 ctcgatgcgg ccgtggcgct ggagccggcc acaagcgtcg cgttgctgaa tcagctgcgc
    22561 gaggcatggc tgcgcagcgc aggccaggac cgagcgcctt gatttcctgg ttcactctcg
    22621 atcgactcga ccgctactca ctgcgactga tcacaggtcc gttcgcgatg gcgctggtgg
    22681 cgctgctgct ggcacaactg ctggagcgcc tgctcagact gttcgacctg gcagcctctg
    22741 ccggtgcacc tccttcaagc gtgctggtga tggcggccac ccttgtgccg cactacctcg
    22801 gcctggcgct gccgatggcc ttcacggcgg ccatcttcat ggctgcagcg cacatgtgcg
    22861 atgacaacga gctggacgtg atgctggtcg ccggccgttc gatcgcccgc attgcagcgc
    22921 cctactttct gctcgccatg ttcctgtgcg tcttcagcct gtacctctac gggcagctgc
    22981 aaccgctggc acgctatggc tatcacgtcg ccgtgaatcg ggtgctgcag accggatggg
    23041 atgcccgcgt cgaggaaaac cggttcatcg acatcggaca cggcatcacc ttcagtgcgg
    23101 acgtgatcga agggaatggg cgggatctcc gtggcgttct gatggagcgc cggagcgcgg
    23161 gcaacgacga gatcctgacg gctgcacgag ggcacctcgt gcacacgccc gacgggctgc
    23221 aactgcaact cgaggacgga cagatcgtgc gccagcgcga cgtgcctggc ccgagcggga
    23281 cggcgatatc ggtatcgcgg ttcgcgcacg gcttgtccgg gcgcgatttc gcacccgaag
    23341 tggagcccct ggccgatcgc ggtagttcgg tgcgcgaacg caccctccat gaactctggc
    23401 gcgacatgca gcctgacaag gaagcggtcg gccgtgccaa ggcggcggcc gagttccatg
    23461 gccgcctggc tcgggcgatg acgtttccgc tcctgccgct gctggcgctg ccgttgggca
    23521 tggcctccaa gcgcggccga cgcacacccg gcgtcatctt cgcggtgctg gcgctgctgg
    23581 ccatccacca tgcgctgcaa ctcggagaaa gcctggccga atcgggccgc ctgcctgccg
    23641 cggccgccgt gtggacgcca ttcgccgtct tcgcggccct gagcctgtgg atctttcgca
    23701 gcagcctggc ctggccgggt gacaacccgg tgctgcgcgc cgtgatggcc atcgaggcct
    23761 ggttcgatcg cgcgcggacg cgaacccggc cgaagaccgt cgcatgaacg ggcgcctggc
    23821 ttcgtacctg cgccgccgcg tcggcatgca gatcctcgcg ttgctggccg tgctcaccgc
    23881 catgatgcag gtgctcgagt tgctcgacgt gactaccgac atactcgaac gcggtcaggg
    23941 ggtgagcggc ctgctctact acgccgccct gcgcacgccg gctgagctcg cgctggcctt
    24001 gccgctggcg gtcctgctcg gaacgatgac cgcactgaac gccatgtctc gcggcatgga
    24061 gatcactgcg atgcgcatca gcggcatgag cctgatgcga ctgctgcgcg acctcttgcc
    24121 ggtactgctc gccgtggctg ccgtccaggt cgtgctgctg caggcggtgc tgccccgggt
    24181 cgaaattgaa ttgaagcagt ggtggaatgc aaccacaccc cccgagccgg caactgcgcc
    24241 cgaaaaagag acgccgccgc tgtgggcgca tacccgcgac ggtgcggtat cgatagacac
    24301 cttcagtccc gatggccggc aactgcgaga cgttcgcctt tattcgcagc gtgccggcca
    24361 gctcacttcg cgtattcgtg cagccacggc aagctgggac gggcgagcct ggcagctcga
    24421 ggatgtgacg gagctgcgca tcgaggcctc gggggtgcgt gtcatccatg aaaagatgcg
    24481 cgagtggcag tcgaacctgc atcccgagga agtgctgcgc ctgggcctgc cgcgacccta
    24541 cctatccact atgatgcttg ccgacgtgat agccggttcc cgcgtcggat cacaaccact
    24601 cagctactac cagaccgcct tgtaccgttc gttcgcggcg ccgctcggcg tgttcatcat
    24661 gctgctgctg gcactgccga cggccatcac gcctgcacgc gcgacaggcg gtggcgcgat
    24721 gctgctggca ctgacgctgg gtctcggatt cctgctgttc gacggcatcg tggcggcgct
    24781 cggctccagt ggcaaatttc cgccgctcgc cacggccctc accgcaccgc tgctgttcgc
    24841 ctctatcggc ctctggcgcc tgcgggcctg cgaaagccta tgaagatctg gatcgacacg
    24901 ggcgccactc agcgcgccca gacgct
//
LOCUS       DAOORV010000045        24588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2461_length_24588_cov_7.529083, whole genome shotgun sequence.
ACCESSION   DAOORV010000045 DAOORV010000000
VERSION     DAOORV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24588
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_24588_cov_7.529083"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKV56_08055"
     CDS             complement(<1..455)
                     /locus_tag="PKV56_08055"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX67808.1"
                     /translation="MKPNALRRRVTAICLASLTLGMVPASHAADAAIRLGVPVWVGWM
                     PWWVAKEKGLFTKRGVAVELKNFAVQGDAVTALAGGNLEAASLATNDILTINAAGPKA
                     SVVLLHDESAGADMLIVRGIAKPKDLKGQRIALEIGGVSHFFLTKLLAQ"
     gene            complement(506..1462)
                     /locus_tag="PKV56_08060"
     CDS             complement(506..1462)
                     /locus_tag="PKV56_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOX67809.1"
                     /translation="MSGPRKHGLQCVHCTSLHPHWDRSLPPAHVVADGAVVTMTCPDA
                     SNGLLGPKSTSQDIAAIDFTQLDPLCGPIYIEGAEPGDALQVEVLDIQLASWGWSGLL
                     KGFGLLADEFPQAYYRSFDLSAGQVDVFGLPFALRPMIGVLGVAPAQDGAFASITPTV
                     AGGNIDVRYLGVGSTIYLPVFNTGALLSGGDGHALQGEGEISGTAIEAPMTATVRVKV
                     IKQQALTAPVLDFSTARYAETEYRDFLGIGPDLYQAARDATRRAVDALAAHFKVEPYE
                     AYAVLGMVGELRIHEIVDQPNWVVGCMVPRRLFQGRLFPAGA"
     gene            complement(1615..1920)
                     /locus_tag="PKV56_08065"
     CDS             complement(1615..1920)
                     /locus_tag="PKV56_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67810.1"
                     /translation="MQIRHDDHFNREMGCTEADWLRWLPAAVGDRVLSCDRDRRQASI
                     AIGAGRCKLNWAPLSDRQIALMCIPRLAVSFDFEGVREVDRVAFLHHFDLHMQRGGG"
     gene            complement(1969..2223)
                     /locus_tag="PKV56_08070"
     CDS             complement(1969..2223)
                     /locus_tag="PKV56_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOX67811.1"
                     /translation="MKVTFKLYAGLTEYLPPEARTGNRVSIDAADGTTIAQLIEPFGL
                     PEKQVHLVLVNGVYVEPAQRLSRVLEEDDVLAIWPPIAGG"
     gene            complement(2220..3491)
                     /locus_tag="PKV56_08075"
     CDS             complement(2220..3491)
                     /locus_tag="PKV56_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX67812.1"
                     /translation="MKHLILGAGPAGVIAAETIRKYAPLDEITIVGDEPEAPYSRMAI
                     PYLLIGNVGESGTHLRHTDGHFEAQRIALKRGRAKSLDSAARTVTLDDGSVLGYDRLL
                     IATGSSPMRPPIPGIDSPGVHPCWTLEDARAIQQRAVQGARVLQMGAGFIGCIIMEAL
                     AVRGVQLTVVEMGDRMVPRMMGPTAGGMIKDWCQTKGVRVHTGTRVEAIEPVAGSQAL
                     KVRLSSGDTLEVDLVISATGVKPNIGFLQGSGIATKLGVLTDVNLQSNLPGVFAAGDC
                     AEAFDKVTGKTIVSAIQPNAADQAYVAAMNMIGRATELKQVTQINVLDTLGLISTSFG
                     DWEGDPQGQHVELTDRAAGRHLSLQFRGDVLIGCNSIGWTNHVGVMRGLVEGRIPLGP
                     WKDKLLLDPTQLMSAYLARAQAQDQPALVKA"
     gene            complement(3612..5468)
                     /locus_tag="PKV56_08080"
     CDS             complement(3612..5468)
                     /locus_tag="PKV56_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOX67813.1"
                     /translation="MSWTGKILRVNLTAGTVQSEPLNMTWAKQYLGSRGLATKYLVEE
                     IDAKVDPLSPENKIIWATGPLTGTMASTGGRYTVVTKGPLTGAIACSNSGGYWGSELK
                     FAGWDMVIFEGRSAKPVYLSIENDVAELRDAGHLWGKTVWETEETLKKQLQDPLLRIS
                     SIGGAGENGVLYAAVVNDLHRAAGRSGVGAVMGSKNLKAIAVRGTLGVGNLVNPKEFM
                     RVTYEKKKILAENGVTGQGLPTYGTQVLMNVINEIGALPTRNHRDVQFEGAKDISAEA
                     MHTPRSAPGSDGKKPLVTNQACFGCTIACGRIQKMDPSHFTVSNKPQYWGASGGLEYE
                     AAWALGAANGVNDMDALQYANLLCNEHGMDPITFGATIGAVMELYEMGVLTKEQIGVD
                     AKFGSAAALAHFAEITAKGEGFGKEIGLGSKRLTEKYGHPELSMSVKGQEFPAYDARG
                     IQGMGLGYATSNRGACHLRGYTVASEILGIPVKTDPLVTEGKPGLVKAFQDATATFDA
                     AGICIFTSFAWTLADVQPQVAAACGEEFTLDNLNLIGERIWNMERDFNNRAGFTSKDD
                     TLPPRLLNEPAKTGGAKGHVNRLGDMLPEYYDLRGWDADGQLKSDTRERLGL"
     gene            complement(5497..5973)
                     /locus_tag="PKV56_08085"
     CDS             complement(5497..5973)
                     /locus_tag="PKV56_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX67814.1"
                     /translation="MQKTLHINPDKCTGCLQCEMACSFENYATFTPSKSRIKVFDFHH
                     TGRKVPYTCTQCDEAWCLHACPVEAITIDQMTGAKVVSESTCVGCKVCTIACPFGTVN
                     YVQETGKVQKCDLCGGDPACATACPTGAITYVDANWTGLSKMQAWADKLGNQPGAA"
     gene            6169..7308
                     /locus_tag="PKV56_08090"
     CDS             6169..7308
                     /locus_tag="PKV56_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX67815.1"
                     /translation="MNLRPRPNETSTHLPATIGVLSDRLRQAAIGQSHERCVALGLTE
                     TSVPELSPAPFSRLIDSRERHRRLYEQAAPVMEMLFEQIVSTRSIVALTDPQGTILHS
                     VGDSGFLERAQQIALAPGVNWSESTKGTNAIGTALFTETPTIVHADEHYVRANHFLTC
                     SAAPIFDHAGQMLGVLDVSGDHRSYHAHTLAMVTMSARMIENQWFADKFRHGLRLHFH
                     RRPEMLGTMREAMVALSPDGKILGANRSALELLGISAAALRMQGLEAVFGITLAGIVD
                     HCRHGTDEPLKLASVLRHEPDATLYARALFNWPTLWPALATQAPPEAAEPVRSAAAAT
                     TLEAQEVEAVRQAVESAGGNISLAARRLGVARNTVYRKLAIATKG"
     gene            complement(7341..8441)
                     /locus_tag="PKV56_08095"
     CDS             complement(7341..8441)
                     /locus_tag="PKV56_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX67816.1"
                     /translation="MHEVTIHPQWTIRSPEGAALAPRVVDLLVQLHEHGSLSAACRAS
                     GASYRHAWDLIRQGEQLFGQPLVQMIRGQGSTLTALGEKLVWADRRIHARLSPLLDTL
                     ASELGAEIQKLLSPQPALLRIRASHGFAIEVLHHMLLADGVVSDLKYCGSLEALSSLG
                     DAGSDLAGFHVPEGAFEAPTIAYYSAWLAPRSQKLIHVATRRQGLMVAAGNPKRIYGL
                     PDLTRAGVRFINRQPGSGTRFLFDLLLKEHGIVPAAIHGYEQCEYTHAAVAAFVASGM
                     ADVGCGVETPARQFKLDFIPGHSERYFLLCDERSFDTPDVQRTVALLRGDAFKDAVNR
                     LPGYRAEDSGKVVLLDEVFESLRSVPARRKRR"
     gene            8657..9172
                     /locus_tag="PKV56_08100"
     CDS             8657..9172
                     /locus_tag="PKV56_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOX67817.1"
                     /translation="MTASGIPIAADAHVDTAAAVRDALARYQSTPGALLPILHAVQDA
                     IGHIPHEAVPQIALALNLSRAEVHGVVTYYHHFRATPPGRHLIQVCRAEACQAMGADA
                     LIAHATKRLGCEMHGSSADGSYTLEPVYCLGLCASSPALTWGDELHARVTAESFDRLV
                     ASTSKNPGSAP"
     gene            9169..10740
                     /locus_tag="PKV56_08105"
     CDS             9169..10740
                     /locus_tag="PKV56_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOX67818.1"
                     /translation="MTITVRVPRDSAALAVGADRVAHRIAEVAAARGIDIRLVRNSTR
                     GLFWLEPLVEVTTSAGRVGYGPVEVDDIEAMFDAGFHLGRPHTKGMGLIDELPYLKRQ
                     ERLTFARMGITDPLSLSDYEAHEGWSGLRRALAMPPADIVQAVLDSGLRGRGGAAFPA
                     GIKWKTVAAAAAAQKYIVCNADEGDSGTYSDRMTMEGDPYMLIEGMTIAGLAVGATVG
                     YLYVRSEYPHAIAALNDAIDRAESAGFLGDNLLGSGRAFRLFVRKAAGAYVCGEETAL
                     LESLEGRRGIVRAKPPLPALEGLFGQPTVVNNVITLAAVPLILSRGAAHYRDYGVGRS
                     RGTLPIQLAGNIRRGGLVEKAFGVTLRELLYDFGGGSASGRPIKAVQVGGPLGAYVPE
                     SQWDLPLDYEAYAAVGAVVGHGGIVVHDDTADLSKLARYAMEFCAIESCGKCTPCRIG
                     STRGVEVIDRISAGVQRPQQVQLLRDLCDTMLHGSLCAMGGMTPYPVLSALNHYPQDF
                     GIAAAAPSVADRQPA"
     gene            10751..13657
                     /gene="fdhF"
                     /locus_tag="PKV56_08110"
     CDS             10751..13657
                     /gene="fdhF"
                     /locus_tag="PKV56_08110"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOX67819.1"
                     /translation="MLDHLRDLDHGTPKRQSEQEVTLDIDGTQVTVPKGTSLMRAAVE
                     AGIQVPKLCATDSLEPFGSCRLCLVEIEGRKGYPASCTTPAEAGMKVRTQTPKLQELR
                     KGVMELYISDHPLDCLTCGANGNCELQDMAGVTGLREVRYGFDGANHTKSAKDESNPY
                     FTYDPSKCIVCNRCVRACEETQGTFALTISGRGFESRVSPGQDEPFMQSECVSCGACV
                     EACPTATLQEKSVIWLGQGEHSVTTTCAYCGVGCGFKAEMKGSEVVRMVPWKDGQANE
                     GHACVKGRFAWGYATHKDRMLKPMIRAKITDPWQEVSWDEAIGHAASEFRRIQAQHGR
                     DSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQ
                     TFKSVDQADVIMVIGANPTDGHPVFASRMKRRLRGKNNAPGAKLIVVDPRRIDLIRSP
                     HVQADHHLQLKPGTNVAVITAMAHVIVTEGLVDEAFVAARCETKSFNEWRAFVAQPVN
                     SPEAVESITGVPAGELRAAARLFATGFDGPDGRRRNGAIYYGLGVTEHSQGSTMVMGI
                     ANLAMATGNVGREGVGVNPLRGQNNVQGACDMGSFPHELPGYRHVSDATARASFEAAW
                     GVDINPEPGLRIPNMFDAALTGSFKGLYCEGEDIVQSDPNTQHVAAAMLAMECVVVQD
                     LFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKAGVADWEVTVKLASA
                     LGYPMHYTHPEQVMDEIAALTPTFKGVSYAKLDQLGSIQWPCNDEAPGGTPTMHIDHF
                     VRGKGKFIITQYVATDEKVTRRFPLLLTTGRILSQYNVGAQTRRTENNQWHSEDRLEI
                     HPHDAEERGIRPDDWVGIQSRAGETVLRATVTERMQPGVVYTTFHFPESGANVITTDN
                     SDWATNCPEYKVTAVQVMPVMQPSVWQQEYSRFNRLQHELLDARIDAVDATPTR"
     gene            13668..14546
                     /gene="fdhD"
                     /locus_tag="PKV56_08115"
     CDS             13668..14546
                     /gene="fdhD"
                     /locus_tag="PKV56_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOX67820.1"
                     /translation="MKPNDALLMIGGAARQLPVEGVRQGRPFAWNDWVAEEVPVALEF
                     NGIVHAVMLATPLDLEDFALGFALSEGLLAGREELYGIDEEPSADGITLKLDVSGAAF
                     ARLKDRRRSMAGRTGCGLCGAESLAQVMRPLPALSIPMRSDADPDPGSLQQRGAVARA
                     MTEMQAMQTLQHATGAVHAAAWCKADGEVALLREDVGRHNALDKLIGALTRSRAAGRI
                     EPHSGFIAVTSRASFEMVQKTVMAGVSLLAAVSAPTALAVRTADSCGLTLIGFARDKD
                     LVVYSHPSRLNASGAY"
     gene            14571..14795
                     /locus_tag="PKV56_08120"
     CDS             14571..14795
                     /locus_tag="PKV56_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HOX67821.1"
                     /translation="MHTDTLIQMANQIGAFFEAMPDREEALEGIAMHIKRFWEPRMRR
                     EFLAYVDAGGAAELHAVVAQAVRLHRGLLA"
     gene            complement(14892..15302)
                     /locus_tag="PKV56_08125"
     CDS             complement(14892..15302)
                     /locus_tag="PKV56_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HOX67822.1"
                     /translation="MSVVLDQGALAQAPLQVTATAATLDLLGRVVAQHGPVMFHQSGG
                     CCDGSAPMCYPAGEFLIGDQDKLLGYIGGMPFYISASQFEYWKHTQLIIDVVPGRGGM
                     FSLEGSLGLRFLTRSQLFTDDEFDALVAQGRASV"
     gene            complement(15299..16819)
                     /locus_tag="PKV56_08130"
     CDS             complement(15299..16819)
                     /locus_tag="PKV56_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOX67823.1"
                     /translation="MDMLEPGKFGTAVAFKKRYGNFIGGQWVAPAKGQYFENITPITG
                     KAFCEIPRSTAEDVERALDAAHAAKAAWGKTSPTERANILNKIADRLEANLPMLAAAE
                     TWDNGKPIRETTFADLPLAVDHFRYFAGCVRAQEGAISTIDDKTYAYHFHEPLGVVGQ
                     IIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPVSILVLVETIADLLPPGVLNVVNG
                     FGLECGKPLASSPRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNVFFADVM
                     DKDDAFFDKALEGFALFALNQGEVCTCPSRALIQESIYERFIERAVKRVAAIKQGNPL
                     DMETMIGAQASSEQLEKIVSYLDLGKQEGAQCLTGGARTVMTGDLAGGYYVQPTVFKG
                     HNKMRIFQEEIFGPVLSVTTFKDEEEALAIANDTLYGLGAGVWSRNGNVAFRMGRGIQ
                     AGRVWTNCYHQYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYDENKMGFF"
     gene            complement(17114..17938)
                     /locus_tag="PKV56_08135"
     CDS             complement(17114..17938)
                     /locus_tag="PKV56_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX67824.1"
                     /translation="MNLQRRFFIGALLAVCGVAASAQERSIVMASTTSTEQSGLFAHL
                     LPAFKAARNIDVKVVAVGTGQALDMGRRGDADVLFVHDQVAEEKLVAEGFGLKRMPVM
                     YNDFVVVGPKADPAGVKGKDVAEALRKLSTASDTSFVSRGDKSGTHAAELRFWKAVGI
                     DMPAGKVPGYKECGCGMGPALNIASSTNAYALTDRGTWLNFKNRGDLAVLVEGDTKLF
                     NQYGVIVVNPAKHPHTKTELAQAFADWVVSADGQAAIAAYKIGGEQLFFPNANAKP"
     gene            complement(17938..18684)
                     /locus_tag="PKV56_08140"
     CDS             complement(17938..18684)
                     /locus_tag="PKV56_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX67825.1"
                     /translation="MSAQLTPASWPMSIGAPLVELGAASVALGARTVLDRIDLQVRSG
                     DRIALVGANGAGKTTLLRALHGLVPLTSGHRRTAGDHAGLRIAMVFQRPFMLRASLLA
                     NIELGLRLQGLSAMERRESALHALEQVGLAALAQHPGRALSGGQQQRAALARAWALKP
                     QLLLLDEPTASLDPSAKREVEDLIGGFAAQGITVVMCSHNLGQVKRLSRRVIYLEAGR
                     VLADVETDHFFHGLLPSEAALFLKGELPWV"
     gene            complement(18681..19397)
                     /locus_tag="PKV56_08145"
     CDS             complement(18681..19397)
                     /locus_tag="PKV56_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67826.1"
                     /translation="MTAFFDSLLAILQLVTSGDPRLWKIVGLSLGVSAAACAIATTAG
                     LLLGAAVAVGQFRGRRVVLVLMNTLLALPSVVVGLMVYLLLSRAGPLGSMGILFTPSA
                     MVVAQAILVTPIVAALARQTIEDAMRDHGEQLLSLGASRFACALIIAWDERLVLLTLA
                     LVAFGRAVSEVGAVMIVGGNIDGVTRVMTTAIALETSKGDLPLALALGFVLLAVVLLL
                     NTLVALMRDWRAPGDARIAA"
     gene            19547..20626
                     /locus_tag="PKV56_08150"
     CDS             19547..20626
                     /locus_tag="PKV56_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX67827.1"
                     /translation="MNEVAADLHHPLMTMLDAIHRSGSISGAARLLGLSYRHVWGELK
                     RWEIELGDTLVIWSKGQPATLSPFGAKLLWAERHAQARLAPQIEAMRVELQRAFAIAF
                     DDTAGVITLYASHDHALPRLREWVQARHRLHLDIQFTGSVDALAALNEGRCLLAGFHA
                     LTAAPLRSPTAGVYRPMLKPGRHKLIGFAQRNQGLMVAAGNPLNIRSLHDLTRPGLRF
                     VHRPRGTGTRVVLEELLAAQHIKPEQIGGFDRTEPSHQAAAVVVASGSADAAFGIEVA
                     ALDNGLEFVPLARENYFLVTLAAQLGHPHVAELLRALREPAWRSIIESIPGYSADGSG
                     EVLSLRAVLPWWTYRKPKPGAEPPT"
     gene            complement(20631..21923)
                     /gene="senA"
                     /locus_tag="PKV56_08155"
     CDS             complement(20631..21923)
                     /gene="senA"
                     /locus_tag="PKV56_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HOX67828.1"
                     /translation="MNKKAPEKWVSDTTDVDSPLLRCAGKDLLSLALMDSRNHTLQWL
                     SVYESGLDAMDWRVPMRPELNPPLWEAGHIGWFQEHWIGRHVQRQRGVACDPTGIRLA
                     SIEPQADDWYDSSAVPHDSRWSLDLPGLQRTKDYLAETMEITLDLLDATDVETGDPDA
                     ALYFHRLALFHEDMHGEAFAMMAQTLCLTLPDSDDRAPPLLRHASGRSQSVQRPPLTF
                     LPTRWTLGLKRGNGFVFDNETDAHEVAVPEFEIDSQAVTWAQYAEFVEDGGYDDVRWW
                     SAAGWQWVQRAGRRVPRYVDQLRQGVLQRRFGVLVRLPMHQPVVHVSWYEADAWCRWA
                     GRRLPTEVEWEVAAHAGAARGFRWGDVWEWTASTFREYPGFRVDPYRDYSKPWFGTHK
                     VLRGASFATSGRMRHPKFRNFYLPERDEMFCGFRSCST"
     gene            21987..22961
                     /gene="senB"
                     /locus_tag="PKV56_08160"
     CDS             21987..22961
                     /gene="senB"
                     /locus_tag="PKV56_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HOX67829.1"
                     /translation="MSPALAAANNGNWQTASRWARLLRPDFHTRVLGDWHGEPCDALI
                     ALHARRSAHAVAAYTAAHPTRPCIVALTGTDLYRDIRDDSSAQESLRRASHLVVLQEQ
                     GIAALPPAMRAKARVIFQSAPTRQPAPPPGRVLRVIFVGHLRAEKDPMTLLRAASRLA
                     CRRDIRIDLIGDGQDCALAESARLAQATQANFRWLGGRPHAETRRRIQRAHLLVNSSL
                     MEGGAHVILEAVRSGTAVVASHVAGNIGMLGEAHAGYFEVGDDAALAALIETACDDAL
                     FLARLRAAGESRSALFSPSTEKQRLHQLVAESLRHAVIHTQTPQETRP"
     gene            22958..24025
                     /gene="selD"
                     /locus_tag="PKV56_08165"
     CDS             22958..24025
                     /gene="selD"
                     /locus_tag="PKV56_08165"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOX67830.1"
                     /translation="MTDIQPADRPAAPRLTSLAHGGGCGCKIAPGVLSQILKSSSGFP
                     IPKELLVGIETADDAAVYRLNDEQALIATTDFFMPIVDDPRDFGRIAATNAISDVYAM
                     GGTPIFALALVGMPINVLPLETIAAILEGGQSICAEAGIPIAGGHTIDSVEPIYGLVV
                     LGLVHPSRVKRNADARPGDKLVLGKPLGVGVLSAALKKNALAPEGYAQMIASTTKLNT
                     PGIELARLDGVHALTDVTGFGLAGHALELARGAGVRAIIDWARVPLLEGVRELAAQGM
                     VTGASGRNWTSHAPHVCLPAGFADIEIALLNDPQTSGGLLVSCSADKVGEVLEIFHRQ
                     GFAQAAVIGEVTEGPAGLTLR"
     gene            complement(24096..>24588)
                     /locus_tag="PKV56_08170"
     CDS             complement(24096..>24588)
                     /locus_tag="PKV56_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX67831.1"
                     /translation="EAFGVGPHDESFIELRDEFLARYESRMTLQTRIFEQVESLLDAL
                     DARAVPWGIVTNKAIRYADPLTRALGLHERASVVIGGDTTPHSKPHPAPLLEAARRMQ
                     IDPACCLYVGDDIRDVQAGQAAGMCTVAVTWGYLGVGDPVESWGAHHTVDIAMDVLNL
                     LAT"
BASE COUNT         4322 a         8282 c         7996 g         3988 t
ORIGIN      
        1 tgctgggcca gcagcttggt caggaagaag tggctgacgc cgccgatctc gagcgcgatg
       61 cgctggccct tcaggtcctt gggcttggcg attccgcgca cgatcagcat gtcggcaccg
      121 gccgactcgt cgtgcagcag cacgaccgag gccttggggc ccgccgcgtt gatggtgagg
      181 atgtcgttgg tggcgaggct ggcggcctcg aggttgccgc cggccagcgc ggtgaccgca
      241 tcgccctgca ccgcgaagtt cttcagctcg accgcgactc cgcgcttggt gaacaggccc
      301 ttctccttgg ccacccacca gggcatccag cccacccaca ccggcacgcc caggcggatg
      361 gcggcatcgg ccgcgtggct ggccggcacc atgccgagag tcaggctggc caggcagatg
      421 gcggtgacgc ggcggcgcag ggcgttgggc ttcatgggag tactccggtt gaaacaagca
      481 ggaaagcgat cggcgttggg ctcgctcagg ccccggccgg gaaaagacgg ccctggaaca
      541 gccggcgcgg caccatgcag ccaaccaccc agttgggctg gtcgacgatc tcgtggatgc
      601 gcagctcgcc caccatgccc agcaccgcat aggcttcgta gggctcgacc ttgaagtgcg
      661 cggcgagcgc atcgaccgcg cgtcgggtgg catcgcgcgc ggcctggtaa agatcggggc
      721 cgatgccgag gaaatcgcgg tactccgtct cggcgtagcg agcggtcgag aagtcgagca
      781 ccggcgcggt gagcgcctgc tgcttgatga ccttgacgcg cacggtggca gtcatcgggg
      841 cctcgatggc ggtgcccgag atctcgccct cgccttgcag cgcgtgcccg tcgcctcccg
      901 acagcagcgc gccggtgttg aacaccggga ggtaaatcgt ggagcccacg cccaggtagc
      961 gcacatcgat gttgccgccg gcgacggtgg gggtgatcga ggcaaaggcg ccatcttgcg
     1021 caggggccac gcccagcaca ccgatcatcg gccgcagcgc gaaaggcagg ccgaacacat
     1081 cgacctgtcc ggccgacaga tcgaagctgc ggtagtaggc ctgcgggaac tcgtcagcca
     1141 gcaggccgaa gcccttgagc agaccactcc agccccacga cgcgagctgg atgtcgagca
     1201 cctcgacctg caaggcatcg cccggctcgg caccctcgat gtagatcggc ccgcacagcg
     1261 gatcgagctg ggtgaagtcg atggcggcaa tgtcctgcga tgtggacttc gggcccagca
     1321 gcccgtttga cgcatcggga cacgtcatgg tgacgacggc accgtccgcc acgacatggg
     1381 ccggcggcag cgatcgatcc cagtgagggt gcaggctggt gcagtgcacg cactgcaacc
     1441 cgtgcttgcg cggcccgctc atggccgcgc tgcctcacgc agagccgcca aggcagcggg
     1501 acggtaccag caactgaagc ggagtgaaac catggggcgc aagcctaggc tgccgccgcc
     1561 acctggtctt ggaaaatacg cgtgctttgg aaagaacgcg gcagggttgt cggctcagcc
     1621 tccgccgcgc tgcatgtgca gatcgaagtg gtgcagaaat gccacccgat caacctcgcg
     1681 cacaccctcg aaatcgaacg agacggcaag acgcggaatg cacatcagcg cgatctgccg
     1741 atccgacaga ggggcccagt tcagcttgca gcggcccgcg ccgatcgcga tcgaggcctg
     1801 acgccgatcg cgatcgcagg acagcacgcg gtcaccgact gccgctggca gccagcgcag
     1861 ccaatcggct tcggtgcaac ccatctcgcg gttgaagtgg tcgtcgtggc ggatctgcat
     1921 gggcggtgtt cggttgtccg cagcgtaccg accaccgagg ccggcgcatc agccgccggc
     1981 tatcggcggc cagattgcca gcacgtcgtc ctcttccagc acgcggctca gtcgctgcgc
     2041 gggctcgaca tagacgccat tgaccaggac caggtgaacc tgcttctcgg gcaggccgaa
     2101 gggttcgatg agctgggcga tcgtggtgcc gtcggccgca tcgatagaca cccggttgcc
     2161 ggtgcgcgct tcgggcggca ggtactcggt gaggcccgcg tagagcttga aggtgacctt
     2221 caagctttca ccagagcggg ctggtcctgc gcctgcgccc gcgccaggta ggccgacatc
     2281 agctgcgtcg gatcgagcag cagcttgtcc ttccagggcc cgaggggaat gcggccctcg
     2341 accagcccac gcatgacgcc cacgtggttg gtccagccga tgctgttgca gccgatcagc
     2401 acgtcgcccc tgaattgcag gctgaggtgc cgccccgccg cgcgatcggt cagttcgacg
     2461 tgctggccct gggggtcgcc ttcccagtcg ccgaagctgg tcgagatcag gcccagcgtg
     2521 tcgagcacgt tgatctgggt cacctgcttc agctcggtcg cccggccgat catgttcatc
     2581 gcggccacgt aggcctggtc ggcggcgttg ggctggatcg cgctgacgat ggtcttgcca
     2641 gtcaccttgt cgaaggcttc ggcgcaatcg cctgccgcaa agacaccggg caaattgctc
     2701 tgcaggttca cgtcggtcag cacaccgagc ttcgtcgcga tgcccgagcc ctgcaggaag
     2761 ccgatgttgg gcttgacgcc agtggcactg atcaccaggt cgacctcgag cgtatcgccg
     2821 ctcgacagcc gcaccttcag tgcctggctg ccggccaccg gctcgatggc ctcgacccgg
     2881 gtgccggtgt gcacgcgcac gcccttggtc tggcaccagt ccttgatcat gccgccggcg
     2941 gtcgggccca tcatgcgcgg caccatgcgg tcgcccatct cgaccacggt gagctgcacg
     3001 ccgcgcaccg ccagcgcttc catgatgatg cagccgatga aacccgcgcc catctgcagc
     3061 acgcgggcgc cctgcaccgc acgctgctgg atcgcgcgtg catcctcgag cgtccagcac
     3121 gggtgcacgc cggggctgtc gatgcccgga atcggcgggc gcatcgggct cgatccggtg
     3181 gcgatcagca gccggtcgta gccgagcacg ctgccgtcgt ccagcgtgac cgtgcgagca
     3241 gcactgtcaa gcgacttcgc gcggccccgc ttcagcgcga tgcgctgcgc ctcgaaatgc
     3301 ccgtcggtgt ggcgcaggtg cgtgccgctt tcgccgacgt tgccgatcag cagataggga
     3361 atcgccatgc gcgaatacgg cgcctcgggc tcgtcgccaa cgatggtgat ctcgtcgagc
     3421 ggcgcgtact ttcggatcgt ctcggcagcg atgactccgg cgggtccggc gcccaggatc
     3481 aggtgtttca tgtgggtatc cctcagaaca ggaagacttc agtcggtgcc gggacgcatt
     3541 gtcgaatggc tgccatcaaa cgcgaaaggc ggtttctcaa ccgcctttca acgtcgtcgg
     3601 atgagtcgcg ctcagagccc cagacgctcc cgcgtgtcgc tcttcagttg gccatcggca
     3661 tcccagccac gcagatcgta gtactcgggc agcatgtcac cgagtcggtt cacatggccc
     3721 ttggcaccac cggtcttcgc gggctcgttc agcaggcgcg gcggcagcgt gtcgtccttc
     3781 gacgtgaagc cggcgcggtt gttgaagtca cgctccatgt tccagatgcg ctcgccgatc
     3841 aggttcaggt tgtcgagcgt gaactcctct ccgcaagcgg ccgcgacctg cggctgcacg
     3901 tcggccagcg tccaggcaaa gctggtgaag atgcagatgc cggcggcgtc aaaagtagcc
     3961 gtggcgtcct ggaatgcctt gaccagaccc ggcttgcctt cggtgaccag cggatcggtc
     4021 ttgaccggga tgccgaggat ctcggaagcc acggtgtagc cgcgcaggtg gcaggcaccg
     4081 cggttgctgg tggcgtagcc caggcccatg ccctggatgc cgcgggcgtc gtaggccggg
     4141 aactcctggc ccttgacgct catgctcagt tcggggtggc cgtacttttc ggtcaggcgc
     4201 ttgctgccca ggccgatttc cttgccgaag ccctcgcctt tcgccgtgat ctcggcaaag
     4261 tgcgccagcg cagcggccga gccgaacttg gcatcgacac cgatctgttc cttcgtgagc
     4321 acgcccatct cgtagagctc catcaccgcg ccgatggtgg cgccgaaggt gatcgggtcc
     4381 atgccatgtt cgttgcacag caggttggcg tactgcaacg cgtccatgtc gttgacgccg
     4441 ttggccgcgc ccagcgccca ggcggcttcg tattcgaggc cgccggaagc gccccagtac
     4501 tgcggcttgt tcgacacagt gaagtgcgac ggatccatct tctggatgcg gccgcaggcg
     4561 atggtgcaac cgaagcaggc ctggttggtc accagcggct tcttgccgtc gctgcccggg
     4621 gccgagcgcg gggtgtgcat ggcctcggcc gagatgtcct tggcgccttc gaactgcacg
     4681 tcgcggtggt tgcgcgtggg cagcgcgccg atctcgttga tgacgttcat cagcacctgg
     4741 gtgccgtagg tcggcaggcc ctggccggtg acgccgttct cggcgaggat cttcttcttc
     4801 tcgtaggtga cccgcatgaa ctccttcgga ttcaccaggt tgccgacgcc cagcgtgccg
     4861 cgcactgcaa tggctttcag gttcttcgag cccatcaccg cgccgacacc cgagcggccg
     4921 gccgcgcggt gcaggtcgtt gaccaccgcc gcgtagagca caccgttctc accggcgccg
     4981 ccgatgctgg agatccgcag cagcggatcc tggagctgct tcttcagcgt ctcctcggtt
     5041 tcccagaccg tcttgcccca gaggtggccg gcatcgcgca gctcggccac gtcgttctcg
     5101 atggacaggt acaccggctt cgccgacctg ccctcgaaga tcaccatgtc ccagccggcg
     5161 aacttgagct cggagcccca gtacccgccc gagttggagc aggcgatcgc gccggtcagc
     5221 gggcccttgg tcaccaccgt gtagcggccg ccggtcgagg ccatcgttcc ggtcagcggg
     5281 ccggtggccc agatgatctt gttttccggc gacagcggat cgaccttggc gtcgatctct
     5341 tccaccaggt acttggtggc caggccgcgc gagccgagat actgcttcgc ccaggtcatg
     5401 ttcagcggct cggattggac cgtgcccgca gtgaggttca ctcgaagaat ctttcccgtc
     5461 caagacatgt tggaactcct gtactggttg gggtgatcag gctgcgccag gctggttgcc
     5521 cagcttgtcg gcccaggcct gcatcttgga caagccggtc cagttggcgt cgacgtaggt
     5581 gatggcgccg gtcgggcacg cggtggcgca tgcagggtcg ccgccacaca ggtcgcactt
     5641 ctggaccttg ccggtttcct gcacgtaatt gaccgtgccg aacgggcagg cgatcgtgca
     5701 gaccttgcat ccgacgcagg tggactcgct gaccaccttg gcgccggtca tctggtcgat
     5761 cgtgatcgcc tccaccgggc aggcatgcag gcaccaggcc tcgtcgcact gtgtgcaggt
     5821 gtagggcacc ttgcggcccg tgtgatggaa atcgaacacc ttgatgcgcg acttgctggg
     5881 cgtgaaggtg gcgtagttct cgaacgagca ggccatttcg cactgcaggc agcctgtgca
     5941 cttgtccgga ttgatgtgga gggtcttctg catgacggcc tttcggaggc agataaggtt
     6001 gccgactatc ttatgaaatc atctgcctag gcccagaccg agagaacccg ggggtaacca
     6061 ctaggcatcg tcggatttcc ggcatgtggc cagcaggggt gtgccaagcc tgaacaaccc
     6121 accgggcatc gcttcgtcca aaatgcgggt ccgccagaaa gaccgccgat gaacctgcgt
     6181 ccccgaccca acgaaacctc gacacacctg ccagcgacga tcggcgtgct gtcggaccgc
     6241 ctgcggcagg cggcgatcgg ccagtcccat gagcgttgcg tcgcgctcgg gctgaccgag
     6301 acttcggtgc ccgagctgag ccccgcacct ttttcaaggt tgatcgacag ccgcgagcgc
     6361 caccgccgcc tgtacgaaca ggcagctccg gtgatggaaa tgctgttcga gcagatcgtg
     6421 tcgacacgca gcatcgttgc gttgaccgat ccgcagggaa cgatcctgca ttcggtgggc
     6481 gattcgggct tcctggaacg cgcccagcag atcgcgctgg cgccgggagt caactggtcg
     6541 gagtcgacca agggcaccaa tgccatcggc acggcgctgt tcaccgagac gcccaccatc
     6601 gtgcacgccg acgagcacta tgtgcgcgcc aaccacttcc tgacctgctc ggcggcgccg
     6661 atcttcgacc acgcgggcca gatgcttggg gtgctcgatg tgtcgggcga ccaccgctcg
     6721 taccacgctc acacgctggc gatggtgacg atgtcggcgc ggatgatcga gaaccagtgg
     6781 ttcgccgaca agttcaggca cggcctgcgg ctgcacttcc atcgccgccc cgagatgctg
     6841 ggcacgatgc gcgaggcgat ggtcgcgctc tcgcccgacg ggaagatcct gggcgccaac
     6901 cgcagcgcgc tagaactgct gggcatcagc gctgccgcgc tgcgcatgca ggggctggaa
     6961 gccgtgttcg gcatcacgct ggccggcatc gtcgaccact gtcgccacgg caccgacgaa
     7021 ccgctgaaac tggcttcggt cctgcgacat gagccagacg caacgctcta tgcacgcgca
     7081 ctgttcaact ggcccaccct gtggccggca ctggcaacac aggctccgcc cgaagcagcg
     7141 gagccggttc gctcggcggc agcggcaacc acgctcgaag cacaggaagt cgaggccgtg
     7201 cgtcaggccg tcgaatcggc aggcggcaac atctcgctgg ccgcacgccg gctcggggtg
     7261 gcacgcaaca ctgtctaccg caaactggcc atcgcaacca agggctgaag cctgaagccc
     7321 gacgctcctg cggagcgccc tcagcgtcgc ttccttctgg ccggaacact ccgcagcgac
     7381 tcgaaaacct cgtcgagcag caccaccttg ccggagtctt cggcgcgata gccgggcaac
     7441 cggttgaccg catccttgaa cgcgtcaccg cgcagcaacg cgactgtccg ctggacatcc
     7501 ggcgtgtcga acgagcgttc atcgcacagc aggaagtagc gctcgctgtg gccgggaatg
     7561 aaatcgagct tgaactgccg cgccggcgtt tcgaccccgc agcccacgtc ggccatgcca
     7621 ctggcgacaa aggcggcaac cgccgcatgg gtgtattcgc actgctcgta gccatggatc
     7681 gccgcaggca cgatgccgtg ctccttgagc agcagatcga acaggaagcg cgtacccgag
     7741 cccggctggc ggttgatgaa gcgcacgccc gctcgcgtca gatcggggag accgtagatc
     7801 cgcttcgggt tgcccgcagc caccatcagc ccctgccgcc gcgtggcgac atggatcagc
     7861 ttctgcgagc gcggagccag ccaggccgag tagtaggcga tggtcggcgc ctcgaatgca
     7921 ccctcgggca cgtggaaccc ggcgagatcg ctgcccgcat cgcccagcga tgacaacgct
     7981 tccaggctgc cgcagtactt caggtcgctc accacgccat cggccaggag catgtgatgc
     8041 agcacctcga tcgcgaatcc atggctcgca cgaatgcgca ggagtgcagg ctgcggggac
     8101 agcaacttct ggatctccgc acccagctcc gaagccagcg tgtcgagcag gggcgacaag
     8161 cgcgcgtgga tgcggcgatc ggcccacacc agcttttcgc ccagggcggt caacgtggaa
     8221 ccctggccgc gaatcatctg caccagcggc tgcccgaaga gttgctcgcc ctggcgaatc
     8281 agatcccagg cgtgccgata cgacgcaccc gatgccctgc acgcggcgga cagactgccg
     8341 tgctcatgca actgcaccag caaatcgacc acccgcgggg caagcgccgc cccctccgga
     8401 gagcggatgg tccactgggg atggatcgtg acttcgtgca tctgacgcct gacctagggt
     8461 gcgtcttgca tagcgcttga gcctccgggc tcatattgca gacgcagcca tcgaactcta
     8521 cagtgcgacg cacaaaacaa gttcagcagg aagcgcatcg acccaaggtc cggatcgctt
     8581 atgttgaaca cagcctatag agacaagcgc cttgtcgttc gcagccgaac gtcgcgccac
     8641 aggtcaggac catccaatga ctgcttccgg cattccgata gctgccgacg ctcacgtcga
     8701 caccgctgcg gcggtgcgcg atgcgcttgc ccggtaccaa agcacccccg gtgcgctgct
     8761 gccgatcctc catgcagtgc aggatgccat cggccacatt ccccacgagg ccgtgccgca
     8821 gatcgcgctc gcgctgaacc tgtcgcgcgc cgaggtgcac ggggtcgtca cttattacca
     8881 ccacttccgt gccacgccgc ccggccgcca tctgatccag gtctgtcgcg cggaagcctg
     8941 ccaggccatg ggtgccgatg cgctgatcgc gcacgcgacg aagcgcctgg gatgtgaaat
     9001 gcacggctcc agcgccgacg gcagttacac gctcgagccg gtctactgcc tcggcctgtg
     9061 cgcgtcgtcg ccggccctga catggggcga cgaactgcat gcgcgggtca cagcggaaag
     9121 cttcgaccga ctggtcgcgt caacatcgaa gaatcccggg agcgccccat gacgatcaca
     9181 gtccgcgtgc cgcgcgactc ggcagcactg gcggtgggcg ccgaccgcgt cgcccatcgc
     9241 atcgcggaag tggcagcagc tcgcggcatc gacatcaggc tggtgcgcaa cagcacgcgc
     9301 ggcctgttct ggctcgaacc gctggtggag gtcaccacct ctgcaggtcg cgtcggatac
     9361 ggcccggtcg aggtcgacga catcgaggcg atgttcgacg ccggattcca cttgggccgc
     9421 ccgcacacca agggcatggg actgatcgac gagttgccct acctgaagcg gcaggagcga
     9481 ctcacgttcg cccgcatggg gatcaccgac ccgctgtccc tgtccgacta cgaggcccac
     9541 gaaggctggt cggggctgcg ccgcgcgctg gcgatgccgc ctgccgacat cgtgcaggcc
     9601 gtgctcgact cgggcctgcg cggccggggc ggcgcggcat tcccggccgg catcaagtgg
     9661 aagacggtgg ctgccgcagc cgccgcacag aagtacatcg tctgcaacgc cgatgaaggc
     9721 gactccggca cctattccga ccgcatgacg atggagggcg atccgtacat gctgatcgaa
     9781 ggcatgacga tcgccggact cgcggtcggc gccaccgtcg gctacctcta cgtgcgttcc
     9841 gaatatccgc acgccatcgc tgcgttgaac gatgccatcg accgcgccga gtcggccggc
     9901 ttcctcggcg acaacttgct cggatctggc cgggcgttca ggctcttcgt gcgcaaggcc
     9961 gccggcgcct atgtctgcgg tgaagagaca gcgctcctcg aaagcctgga aggcaggcgc
    10021 ggcatcgtgc gcgccaagcc gccgctgccg gcgctcgagg ggcttttcgg ccagccgacc
    10081 gtcgtcaaca acgtcatcac gctggctgcg gtgccgctga tcctgtcgcg tggcgcagcg
    10141 cattaccgcg actacggggt aggacgatcg cgcggcacgc tgccgatcca gctcgcgggc
    10201 aacatccggc gcggcggcct ggtcgaaaag gccttcggcg tgacgctgcg cgaactgctc
    10261 tacgacttcg gcggtggctc ggcctcgggc aggccgatca aggcggtgca ggtaggcggg
    10321 ccgctcggcg cgtacgtgcc cgaatcgcag tgggacctgc cgctggacta cgaagcctat
    10381 gcggcggtcg gcgccgtggt cggccacggt ggcatcgtgg tgcacgacga caccgccgac
    10441 ctgtcgaagc tggcgcgtta cgcgatggag ttctgcgcga tcgaatcgtg cggcaagtgc
    10501 acaccgtgcc gcatcggctc gacgcgcggg gtcgaggtga tcgacaggat ttcggctggc
    10561 gtccagcggc cgcagcaagt gcagctgttg cgcgacctgt gcgacacgat gctccacggc
    10621 agcctgtgcg cgatgggcgg catgacgccc taccccgtgc tgtcggcgct gaaccattac
    10681 ccgcaggact tcggtatcgc cgctgccgct cccagcgtcg ccgaccgcca gcctgcttga
    10741 ggagacagac atgctcgacc acctcagaga cctcgatcac ggcacgccga agcgccaaag
    10801 cgaacaggag gtcaccctag acatcgatgg cacgcaggtg acagtgccga agggcacctc
    10861 gctgatgcgc gccgcagtcg aagcgggcat ccaggtgccc aagctgtgcg cgaccgacag
    10921 cctcgaaccg ttcggctcgt gccggctgtg tctggtcgag atcgagggcc gcaagggcta
    10981 cccagcctcg tgcaccacac cggccgaagc cggcatgaag gtgcgcacgc agacgcccaa
    11041 gctgcaggaa ctgcgcaagg gcgtgatgga gctgtacatc tccgaccacc ctctcgattg
    11101 cctgacctgc ggcgccaacg gcaattgcga gctgcaggac atggccggcg tgaccggtct
    11161 gcgcgaggtg cgctacggct tcgatggcgc caaccacacg aagagcgcga aggatgaatc
    11221 gaacccgtac ttcacctacg acccgagcaa gtgcatcgtc tgcaaccgtt gcgtgcgcgc
    11281 ctgcgaggag acacagggca ccttcgcgct gaccatcagc gggcgcggct tcgaatcgcg
    11341 cgtgtcgccc gggcaggacg agccgttcat gcagagcgaa tgcgtcagtt gcggcgcctg
    11401 cgtggaggcc tgcccgaccg cgacgctgca ggagaagtcg gtgatctggc tcggccaggg
    11461 cgagcacagt gtcaccacca cctgcgccta ctgcggcgtg ggctgcggct tcaaggccga
    11521 gatgaagggc agcgaggtcg tgcgcatggt gccctggaag gacggccagg ccaacgaagg
    11581 ccacgcctgc gtgaagggcc ggttcgcctg gggctacgcc acacacaagg accgcatgct
    11641 caagccgatg atccgcgcga agatcaccga cccatggcag gaggtgagct gggacgaggc
    11701 catcggccac gcagccagcg agttccggcg catccaggcc cagcacgggc gcgactccat
    11761 cggcggcatc acctcctcgc gttgcaccaa cgaggaaacc tacctggtgc agaagctggt
    11821 gcgcgcggcc ttcggcaaca acaacgtcga cacctgcgcg cgcgtgtgcc actcgccgac
    11881 cggctacggg ctgaagcaga cgctgggcga atcggccggc acgcagacct tcaagtcggt
    11941 cgaccaggcc gacgtgatca tggtcatcgg cgccaacccg accgacgggc acccggtgtt
    12001 cgcgtcacgc atgaagcgca ggctgcgtgg caagaacaac gcgccgggcg cgaagctgat
    12061 cgtcgtcgac ccgcgccgca tcgacctgat ccgctcgccg catgtgcagg ccgatcacca
    12121 tcttcaactg aagcccggca caaacgtcgc ggtgatcacc gcgatggcgc atgtgatcgt
    12181 caccgagggg ctggtcgacg aggctttcgt cgccgcgcgc tgcgagacga agtcgttcaa
    12241 cgaatggcgc gcgttcgtcg cccagccggt caattctccg gaggcggtgg agtcgatcac
    12301 gggagtaccg gccggcgagt tgcgcgcagc tgcccgcctg ttcgccaccg gattcgatgg
    12361 cccggatggc cgccgcagga acggcgcgat ctactacggc ctcggcgtca ccgagcacag
    12421 ccagggctcg acgatggtga tggggattgc caacctcgcg atggccactg gtaacgtcgg
    12481 ccgcgaaggt gtcggcgtga acccgctgcg cggccagaac aacgtgcagg gtgcctgcga
    12541 catgggctcg ttcccgcacg agctgccggg ctaccgtcat gtgtccgacg ccaccgcgcg
    12601 tgcgagcttc gaggccgcct ggggcgtcga catcaacccc gaaccgggcc tgcgcatccc
    12661 gaacatgttc gacgcggcgc tgaccggcag cttcaagggc ctgtactgcg agggagagga
    12721 catcgtgcag tccgacccga acacgcagca tgtggccgcg gcgatgctgg ccatggaatg
    12781 cgtggtggtg caggacctgt tcctgaacga gaccgcgaag tacgcgcacg tgttcctgcc
    12841 ggggtcgtcg ttcctcgaga aggacggaac cttcaccaat gccgagcgcc gcatctcgcg
    12901 agtacgcaag gtcatgccgc caaaggccgg cgtggccgac tgggaggtga ccgtgaagct
    12961 cgccagcgcg ctgggctacc cgatgcacta cacgcacccc gaacaggtga tggacgagat
    13021 tgccgcgctg acgccgacat tcaagggtgt cagctacgcc aagctcgacc agttgggcag
    13081 catccaatgg ccctgcaacg acgaggctcc cggcggcacc ccgacgatgc acatcgacca
    13141 cttcgtgcgc ggcaagggca agttcatcat cacgcaatac gtggccaccg acgagaaggt
    13201 cacgcgacgc tttccgctgc tgctgaccac cggacgcatc ctgtctcagt acaacgtcgg
    13261 cgcacagacg cgacgcaccg aaaacaacca gtggcacagc gaggaccgcc tcgagatcca
    13321 cccgcacgac gccgaggaac gcggtatccg gcccgacgat tgggtcggca tacagagccg
    13381 tgccggcgaa accgtgctgc gcgcgaccgt caccgagcgc atgcagccgg gcgtggtcta
    13441 cacgacgttc cacttccccg agtcgggtgc caacgtcatc accaccgaca actccgactg
    13501 ggcgaccaac tgtcccgaat acaaggtgac tgcggtacag gtcatgccgg tgatgcagcc
    13561 gtcggtctgg cagcaggagt acagccgctt caaccggctg cagcacgagt tgctcgatgc
    13621 gcgcatcgac gcggtcgacg ccacaccgac ccgctgagga tccgcgaatg aaaccgaacg
    13681 acgcactgct gatgatcggg ggggcggctc gtcagcttcc cgtcgagggc gtgcgccagg
    13741 gccgaccttt cgcctggaac gactgggttg cagaggaagt gccggtcgct ctcgagttca
    13801 acggcatcgt gcacgcggtg atgctcgcca cgccgctcga cctcgaggat ttcgcactcg
    13861 gcttcgcgct cagcgaaggc ctgctggccg gccgcgaaga gctttacggc atcgatgaag
    13921 aaccttcagc cgacggcatc accctgaagc tcgacgtgtc gggcgccgcc ttcgcgcgcc
    13981 tgaaggaccg ccgccgctcg atggccggcc gcactggctg cggactgtgt ggcgcggaaa
    14041 gcctggcgca ggtgatgcgc ccgctgcccg cgctgtcgat tcccatgcga tcggacgcgg
    14101 acccggatcc cgggtcactg cagcagcgtg gtgcagtggc gcgggccatg accgagatgc
    14161 aagcgatgca gaccctgcag cacgccaccg gtgccgttca tgctgcggca tggtgcaagg
    14221 ccgacggcga agtcgccttg ttgcgcgaag acgtcggccg ccacaacgcg ctcgacaagc
    14281 tgatcggcgc gctgacccgc agccgtgccg ccggccgcat cgaaccgcac agtggcttca
    14341 tcgccgtcac cagccgggcc agcttcgaaa tggtccagaa gaccgtgatg gcaggtgtgt
    14401 cattgctggc tgcggtatcg gcaccgaccg cactggcggt tcgcacagcc gactcctgcg
    14461 gcctgacgct gatcggcttc gcccgcgaca aggaccttgt cgtctacagc cacccctcgc
    14521 gcctcaatgc cagcggcgcc tattgaccca cgcgatcgac cgcgcccccc atgcacaccg
    14581 acaccctgat acagatggcc aaccagatcg gcgccttctt cgaagcgatg cctgaccggg
    14641 aagaagcact ggagggaatc gcgatgcaca tcaagcgttt ctgggaaccg cgaatgcgac
    14701 gcgagttcct ggcgtatgtc gatgccggcg gggctgctga actgcatgca gtggtggctc
    14761 aggcagtgcg actgcaccgc ggcctgctgg cctgagccgg tacgacgaaa ctcgacgggc
    14821 ccaagcaaga agcgcggccc ccctgtcgag gggccgcgcc ttgctaccga tatccgtcgc
    14881 tcgacctcga ctcagaccga tgccctgccc tgcgcaacca acgcatcgaa ctcatcgtca
    14941 gtgaacagct gagagcgcgt caggaaacgc agcccgagag agccctcgag cgagaacatg
    15001 ccgccgcgtc ccggcacgac atcgatgatc agctgggtgt gcttccagta ctcgaactgg
    15061 gaggcgctga tgtagaacgg catgccgccg atgtacccca gcagcttgtc ctgatcgccg
    15121 atcaggaact cgccagccgg atagcacatc ggcgcgctgc catcgcagca accgcccgac
    15181 tgatgaaaca tcaccggtcc gtgctgtgcc acgacgcgcc ccagcaagtc gagcgtcgct
    15241 gcagtcgcgg tgacctgcag cggcgcctgt gccaaggcac cctgatcgag cactacgctc
    15301 aaaagaagcc catcttgttc tcgtcgtagc tgacaagcag gttcttggtc tgctggtagt
    15361 ggtccagcat catcttgtgg ttctcgcggc cgatgcccga ctgcttgtag ccaccgaagg
    15421 cggcgtgcgc cggatactgg tggtagcagt tggtccacac gcggccggcc tggatgccac
    15481 gacccatgcg gaaggccacg ttgccgttgc ggctccagac gccagcgccc agaccgtaca
    15541 gcgtgtcgtt ggcgatggcg agcgcttctt cctcgtcctt gaaggtcgtg accgaaagca
    15601 ccgggccgaa gatctcttcc tggaagatgc gcatcttgtt gtggcccttg aacacggtcg
    15661 gctgcacgta gtagccacca gcaaggtcac ctgtcatcac cgtgcgggca ccaccggtca
    15721 ggcactgcgc accctcctgc ttgccgaggt cgaggtagct gacgatcttc tcgagctgct
    15781 cgctcgaagc ctgcgcgccg atcatcgttt ccatgtcgag cgggttgccc tgcttgatcg
    15841 ctgcgacgcg cttgacggcg cgctcgatga agcgctcgta gatcgattcc tggatcagcg
    15901 cgcgcgacgg gcaggtgcag acctcgccct ggttcagggc gaacagcgcg aagccttcga
    15961 gcgccttgtc gaagaaggcg tcgtccttgt ccatcacgtc ggcgaagaac acgttgggcg
    16021 acttgccgcc gagctcgagc gtgaccggga tcaggttctg gctggcgtac tgcatgatca
    16081 gacggccagt cgtggtctcg ccggtgaacg cgatcttggc gatgcgcggg ctcgacgcca
    16141 gcggcttgcc gcactccagg ccgaagccgt tgaccacgtt cagcacgccg ggtggcaaca
    16201 ggtcggcgat ggtctcgacg agcaccagga tcgacaccgg tgtctgctcg gccggcttca
    16261 gcacgacgca gttgccagcg gccagcgcag gcgccagctt ccacaccgcc atcaggatcg
    16321 ggaagttcca cggaatgatc tgcccgacga cgccgagcgg ctcatggaag tggtacgcgt
    16381 aggtcttgtc gtcgatggtg ctgatggcac cttcctgggc gcgcacgcag ccggcgaaat
    16441 aacggaaatg atcgacggcc agcggcaggt cggcgaacgt cgtctcgcgg atcggcttgc
    16501 cgttgtccca ggtctcggcc gctgccagca tcggcaggtt ggcttcgagg cgatcggcga
    16561 tcttgttgag aatgttggcg cgctcggttg gcgaggtctt gccccaggcg gccttggccg
    16621 cgtgggctgc gtcgagtgca cgctccacgt cctcggcggt cgagcgcgga atctcgcaga
    16681 aagccttgcc ggtgatcggc gtgatgttct cgaaatactg ccccttcgcc ggagcgaccc
    16741 attggccgcc gatgaagttg ccgtagcgct tcttgaatgc cacagcagtt ccgaattttc
    16801 cgggttcaag catgtccatg tcgtgtctcc aaaggtgagg ttaatcgctg gcgagggtcg
    16861 ggcgatcgcg cgccgacgac ggcgaccaag tccccgaaat catcgggccg gacgcgcatt
    16921 gtttggcgct gcgatcaacc tgcgtagcgc aggcgatccc ttatgactcg gcgtgcatag
    16981 cgacgcgagc cagtgcgcat ttcgggatca aaccgacact tctgtggcgc gctgccacac
    17041 ccctgggcag caggggcttt ggaatacggg gcgtgtgcgc tgttttggac cagcggcccc
    17101 gcacgtttgc ccctcagggc ttcgcgttgg cgttcgggaa gaacagttgc tcgccgccga
    17161 tcttgtacgc cgcgatcgcg gcctgtccgt cggcagagac cacccagtcg gcgaaggcct
    17221 gtgccagttc cgtcttggtg tgaggatgct tggccgggtt gacgacgatc acgccgtact
    17281 gattgaagag cttggtgtcg ccctcgacca gaacagccag gtcaccgcgg ttcttgaagt
    17341 tgagccaggt gccgcgatcg gtcagcgcat aggcattcgt cgacgacgcg atgttcagcg
    17401 ccgggcccat gccacagccg cattccttgt agcccggcac cttgcccgca ggcatgtcga
    17461 tgccgaccgc tttccagaat cgcagctcgg ccgcgtgggt tccgctcttg tcgccacgcg
    17521 acacgaagga agtgtcactc gcggtcgaca acttgcgcag cgcttcggcg acatccttgc
    17581 ccttgacacc ggcggggtcg gctttcggtc cgaccacgac gaagtcgttg tacatgacag
    17641 gcatgcgctt gaggccgaac ccttcggcca cgagcttttc ctccgcgacc tggtcgtgca
    17701 cgaacagcac gtcagcatcg ccccgccgtc ccatgtccag cgcctggccc gtgcccacgg
    17761 caaccacctt gacgtcgatg ttgcgggcag ccttgaaggc gggcagcagg tgcgcgaaca
    17821 gtcccgactg ctcggtgctc gtggtcgacg ccatcacgat gctgcgctcc tgcgcgctgg
    17881 ccgcgacgcc acacacggcg agtagcgccc cgataaagaa acgacgttgc aagttcatca
    17941 aacccatggg agttctcctt tcaaaaacaa tgcggcttcc gacggcagca ggccgtggaa
    18001 gaagtgatcg gtctccacat cggccagaac gcggccagct tccagataga taacgcggcg
    18061 actcaggcgc ttgacctgac ccaggttgtg ggagcacatc accacggtga tgccctgcgc
    18121 cgcaaagccg cctatcaagt cctcgacctc gcgcttggcg ctcgggtcga ggctggcagt
    18181 cggctcgtcc agcagcagaa gctgcggctt cagcgcccag gcgcgcgcca gtgcggcacg
    18241 ctgctgctgc ccgcctgaca gcgcacgtcc agggtgctgc gccagagcgg ccagacccac
    18301 ctgctcgagc gcatgcaggg cagactcgcg gcgctccatc gcggacaggc cctgaaggcg
    18361 cagccccagc tcgatattgg cgagcaggct ggcccgcagc atgaagggac gctggaacac
    18421 catcgcaatg cgcagacccg cgtggtcgcc agccgtcctg cgatgcccgg aggtcagggg
    18481 caccaggcca tgcagcgcgc gcagcagcgt ggtcttgccc gcaccattgg caccgaccag
    18541 cgcgatgcga tcaccagatc gcacctggag atcgattcga tccagcacgg ttcgagcacc
    18601 gagcgccacc gaggctgcgc cgagctcgac cagcggcgca ccgatcgaca tcggccagga
    18661 ggccggagtc aactgcgcgc tcatgcagcg atccgggcgt cgcctggtgc ccgccagtcg
    18721 cgcatcaagg cgaccagggt gttcagcagc agcaccacgg ccagcagaac gaaacccagt
    18781 gccagcgcga ggggcagatc gcccttgctg gtctcgagcg cgatcgcggt ggtcatgaca
    18841 cgcgtcacgc cgtcgatgtt gccgccgacg atcatcaccg cgccgacctc ggacaccgcg
    18901 cggccgaatg ccaccagtgc cagggtcagc agcaccaggc gctcatccca ggcaatgatc
    18961 agcgcgcagg cgaaccggct ggcacccagg gatagcagct gctcgccgtg atcacgcatc
    19021 gcgtcctcga tcgtctggcg agccagcgcg gcaacgatgg gcgtgaccag gatcgcctgc
    19081 gcgacgacca tcgcggacgg cgtgaacagg atgcccatcg acccgagcgg accggcgcgc
    19141 gaaagcagca ggtagaccat cagcccgacc accaccgacg gaagcgccag cagtgtgttc
    19201 atcagcacca gcaccacacg gcgcccgcga aactgcccca ccgccactgc agcgccgagc
    19261 agcaaacccg ctgtggtcgc gatcgcgcac gcggcagcac tgacaccgag cgacaggccg
    19321 acgatcttcc agagtctggg atctccgctg gtgaccaact gaagaatggc cagcaggctg
    19381 tcgaaaaacg ccgtcatgaa atcaggaaca gatgaggagc tggaagcgac gcttatccta
    19441 ggtctcaatg acccgtccga ccctatgaag tcgaatgcat aagatttcgc tcaattacgc
    19501 gctggtcaac caccgcacgg agaccttgac ggctggcagg gccgacatga acgaagtcgc
    19561 cgcggacctg catcacccgt tgatgacgat gctcgatgcg atccaccgca gcggctcgat
    19621 ctcgggcgcg gctcgcctgc tcggtctgtc ctaccggcac gtctggggcg aactcaagcg
    19681 ctgggagatc gaactcggcg acacgctggt catctggtcg aaaggacagc cggccacgct
    19741 gtctccgttc ggcgccaagc tgctgtgggc agagcggcac gctcaggcgc gcctggcacc
    19801 acagatcgag gcgatgcgcg tcgagctgca gcgagcattc gcgatcgcct tcgacgacac
    19861 ggcgggcgtg atcacgctgt atgccagtca cgaccacgcg ctgcctcgcc tgcgcgagtg
    19921 ggtgcaggcg cggcatcgcc tgcatctcga catccagttc accggcagtg tcgacgcact
    19981 cgccgcgctc aacgaaggac gctgccttct ggccggcttc cacgcgctga cagcggcgcc
    20041 gctgcgctcg ccgacagcgg gtgtctaccg cccgatgctc aagccgggcc gccacaagct
    20101 gatcggtttc gcgcagcgca accagggtct gatggtcgct gcgggcaatc cattgaacat
    20161 ccgcagcctg cacgacctga cccgtccggg gctgcgcttt gttcatcgac cgcgcggcac
    20221 cggcacacgc gtcgttctgg aggaactgct ggctgcgcag cacatcaagc cggagcagat
    20281 cggcggcttc gaccgcaccg aaccatcgca ccaggcggcc gcggtggtgg tggccagcgg
    20341 cagcgccgat gcggcctttg gcatcgaggt cgctgcgctc gacaacggcc tcgagttcgt
    20401 gccgctggct cgggagaact acttcctggt gacactcgcc gcacagctgg gccacccgca
    20461 tgtcgcggaa ctgctgcgcg cgctgcgcga gccggcttgg cgctcgatca tcgagtccat
    20521 cccgggctac agcgccgacg gcagcggcga agtgctgtcg ctgcgcgcgg tgctgccatg
    20581 gtggacctat cgcaagccga agccaggggc ggagccaccg acctgaggca tcaggtcgag
    20641 caactgcgga agccgcagaa catctcgtcg cgctccggca gatagaagtt gcggaatttc
    20701 gggtgccgca tgcgaccgct ggtggcgaac gaggccccac gcaatacctt gtgcgttccg
    20761 aaccagggct tcgagtagtc gcgataggga tcgacccgga aaccggggta ctcgcgaaag
    20821 gtgcttgcgg tccactccca gacatccccc cagcgaaaac ctcgcgcggc acccgcgtgt
    20881 gcagccactt cccattcgac ctcggtcggc aatcggcgcc cggcccatcg gcaccaggca
    20941 tcggcctcat accagctgac gtgaaccacc ggctggtgca tcggcaagcg caccagcaca
    21001 ccgaagcgcc gctgaagaac gccctgccgc aattggtcga cgtacctcgg aacccgccgg
    21061 cctgcgcgtt gcacccactg ccagccggct gcagaccacc atcgcacgtc gtcgtagcca
    21121 ccgtcctcta cgaactcggc gtactgtgcc caggtgacgg cctgcgagtc gatctcgaac
    21181 tcgggcacag ccacctcgtg ggcatcggtc tcgttatcga aaacgaaacc attgccgcgc
    21241 ttcaggccca aggtccagcg cgtcggcagg aacgtcagcg gcggccgctg caccgattgg
    21301 ctccggcccg acgcatgccg cagcagaggc ggtgcgcggt cgtcgctgtc aggcaacgtc
    21361 aggcacagcg tctgggccat catcgcgaac gcctcgccgt gcatgtcttc atggaacagc
    21421 gccagcctgt ggaaatagag cgcggcatcc ggatcaccag tctcgacatc ggtcgcgtcg
    21481 agcaggtcga gcgtgatctc catcgtctcg gccaggtaat ccttcgtgcg ctgcaggccg
    21541 ggcagatcga gtgaccagcg gctgtcgtgc ggcacggcgg acgagtcgta ccagtcgtcg
    21601 gcctggggtt cgatcgaagc gagccggatg ccggtcgggt cgcaagcgac gccgcgctgg
    21661 cgttggacgt gacgaccgat ccagtgctcc tggaaccacc cgatgtgtcc tgcctcccag
    21721 agtggcggat tcagttcggg gcgcatcggg acgcgccagt ccatcgcgtc gagacctgac
    21781 tcgtagaccg acaaccactg caacgtatga tttctcgagt ccatgagcgc cagcgacagc
    21841 aggtccttgc cggcgcagcg cagcagcggc gaatccacgt ccgtcgtgtc actgacccat
    21901 ttttccggtg ccttcttgtt caagtcgtct ccagcatcgg gccttgcatc gtgatcgcca
    21961 gaactcgcca acccaccgtg ctgatcgtga gtcctgcgct ggccgcagcc aacaacggca
    22021 actggcagac cgcatcacgc tgggcccggt tgttgcgccc cgattttcac acccgggtgc
    22081 ttggcgactg gcacggcgag ccctgtgacg ccttgatcgc gctgcatgcg cggcgctcgg
    22141 cccacgccgt cgcggcctac acggctgcgc acccgacgcg cccctgcatc gtcgcgctga
    22201 ccggaaccga cctgtaccgt gacatccgcg atgactcctc cgcgcaggag tcgctgcggc
    22261 gcgcctctca cctggtggtg ctgcaggagc agggcatcgc tgccctgccg cccgccatgc
    22321 gcgcaaaggc tcgcgtgatc ttccagtcgg cgcccactcg ccaaccggcg ccgccacccg
    22381 gacgggtcct gcgcgtgata ttcgtcggtc acctgcgtgc cgagaaagac cccatgacgc
    22441 tgctgcgagc cgcctcacgg ctcgcgtgcc ggcgcgatat ccgcatcgac ctgattggcg
    22501 atggacagga ctgcgcgctg gccgaatcgg cgcgcctcgc acaggcgacg caggcgaact
    22561 tccgctggct cggtggacgg ccgcatgccg agacgcgccg gcgcatccag cgcgcgcacc
    22621 tgctggtcaa cagcagtctg atggaaggcg gcgcccatgt gatcctcgaa gcggtgcgaa
    22681 gcgggaccgc cgtggtggcc tcgcacgtcg ccggcaacat cgggatgctg ggggaggcac
    22741 acgcaggcta tttcgaggtc ggcgatgatg cagccttggc agcgctgatc gagacagcct
    22801 gcgacgacgc gctgtttctg gcgcgtctgc gcgcagcggg ggaaagccgc tcggcgttgt
    22861 tctctccatc caccgaaaag cagcgactgc atcaactggt cgccgagtca ctgcgacatg
    22921 ccgtgatcca cacccagact ccacaggaaa cccgcccatg actgacatcc agcccgccga
    22981 ccgccctgcc gccccgcgcc tgacctcgct ggcccatgga ggcggctgcg gctgcaagat
    23041 cgcgcccggc gtgctctcgc agatcctgaa gagcagttcg ggcttcccga tacccaagga
    23101 actgctggtg ggcatcgaga ccgcagacga tgcagcggtt taccggctca acgacgagca
    23161 ggcactgatc gcgacgacgg acttcttcat gccgatcgtc gacgacccgc gcgacttcgg
    23221 acgcatcgcg gcgaccaacg cgatcagcga tgtctacgcg atgggcggca ccccgatctt
    23281 cgcgctggcg ctggtcggca tgccgatcaa cgtgctgccg ctcgagacca tcgcagcgat
    23341 cctcgaaggc ggccaaagca tctgcgcgga agccggcatc ccgatcgccg gtggccacac
    23401 gatcgattcg gtcgaaccga tctacggcct ggtcgtgctc gggctcgtac acccgagtcg
    23461 cgtcaagcgc aacgcggacg ccagacccgg ggacaagctg gttctgggca agccgctggg
    23521 cgtcggcgtg ctctcggcgg cgctgaagaa gaacgcgctg gcgccggaag gctacgcaca
    23581 gatgatcgcc agcacaacca aactgaacac acctgggatc gaactggccc gacttgacgg
    23641 cgtgcacgcg ctcaccgacg tcacgggctt cggcctcgcc ggccacgcac ttgaactggc
    23701 ccgcggcgct ggcgtgcggg cgatcatcga ttgggcccgg gtgccgctgc tggagggcgt
    23761 gcgggaactc gcggcccagg gcatggtcac cggggcatcg gggcgaaact ggaccagcca
    23821 cgccccccac gtctgcctgc ctgccggttt cgccgacatc gagatcgcgc tgctcaacga
    23881 tccgcagacc agtggcgggt tgctggtttc atgcagtgca gacaaggtgg gcgaggtgct
    23941 cgagatcttc caccggcaag gcttcgccca ggcggcggtg atcggcgaag tgaccgaggg
    24001 cccggcggga ctgacgctgc gctgagccga acacccaccc gccagggctg agtgctgcga
    24061 cacgcagatg caacgtcggg atcgccaggt gcggctcagg tcgccagcaa gttcaggacg
    24121 tccatcgcga tgtcgacggt gtggtgagca ccccaggact cgacgggatc tccgacccca
    24181 agataacccc aggtgaccgc caccgtgcac atgccggcag cctgtccggc ctgcacgtca
    24241 cggatgtcgt caccgacgta gaggcaacac gccgggtcga tctgcatgcg acgcgcagcc
    24301 tcgagcagcg gcgcaggatg cggcttggag tggggcgtgg tgtctccccc gatcaccacg
    24361 gaggcacgct cgtggaggcc caacgctcgc gtcagtggat cggcataccg aatggccttg
    24421 ttggtgacaa tgccccaggg caccgcccgc gcatccagcg catcgagcaa ggattcgacc
    24481 tgctcgaaaa tccgtgtctg cagggtcatc cgcgattcgt agcgcgccag gaactcatct
    24541 cgaagttcga tgaacgactc gtcatgcggt cccacgccga acgcttca
//
LOCUS       DAOORV010000046        24045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2554_length_24045_cov_9.811672, whole genome shotgun sequence.
ACCESSION   DAOORV010000046 DAOORV010000000
VERSION     DAOORV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_24045_cov_9.811672"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="rfbA"
                     /locus_tag="PKV56_08175"
     CDS             complement(<1..701)
                     /gene="rfbA"
                     /locus_tag="PKV56_08175"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOX67832.1"
                     /translation="MNRKGIILAGGSGTRLHPATLAISKQLLPVYDKPMVYYPLSTLM
                     LAGMRDVLVISTPQDTPRFQSLLGDGSAWGMNLSYCVQPSPDGLAQAFILGRQFVGGG
                     PSALVLGDNIYYGHDFASLLARADGRSSGSTVFAYHVHDPERYGVVEFDAERRALSIE
                     EKPKAPKSNYAVTGLYFYDEQVCDIAASIKPSARGELEITAVNAQYLHQNQLNVEIMG
                     RGYAWLDTGTHDSLM"
     gene            complement(727..1623)
                     /gene="rfbD"
                     /locus_tag="PKV56_08180"
     CDS             complement(727..1623)
                     /gene="rfbD"
                     /locus_tag="PKV56_08180"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOX67833.1"
                     /translation="MKILLLGKGGQVGWELQRSLSLLGEVVACDFDSAGDLKADFSEP
                     ESLAPLVQRVRPDAIVNAAAHTAVDKAESEPDLARRINATGPAVLAREAAALGAILVH
                     YSTDYVFDGSGSQPRTEDAPTAPLSVYGRTKLEAEERIRASGCRHLILRTSWVYAARG
                     GNFARTMLRLAAERDALNVIADQVGAPTGADLLADMTALALPRLRADASLGGTYHCAA
                     AGETSWHGYAQFVIEWARARGQAIQVAPDKIAAIPTSAYPTPAQRPLNSRLGTRKLQS
                     AFGVTLPHWQQGVQRMLTEVLG"
     gene            complement(1620..2678)
                     /gene="rfbB"
                     /locus_tag="PKV56_08185"
     CDS             complement(1620..2678)
                     /gene="rfbB"
                     /locus_tag="PKV56_08185"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOX67834.1"
                     /translation="MTILVTGGAGFIGSNFMLDWLAQSNEAVVNLDALTYAGNLQNLQ
                     SLQGDTRHTFVKGDICDRALVDSLLATHRPRAIVHFAAESHVDRSIHGPGAFMKTNVD
                     GTFTLLEAARGYWSGLDDAAKATFRFHHVSTDEVYGSLSPTAPAFTENHPFEPNSPYS
                     ASKAASDHLVRAWHHTYGLPVVTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGD
                     GQQVRDWLYVGDHCSAIRAVLAGGRLGETYNVGGWNEKPNIEIVKTVCALLDELRPSP
                     EGSYTRLITYVKDRPGHDRRYAIDARKIERELGWRPAETFDTGIRKTVQWYLAHADWV
                     ANVQSGGYRDWVNQQYGA"
     gene            complement(3640..4002)
                     /locus_tag="PKV56_08190"
     CDS             complement(3640..4002)
                     /locus_tag="PKV56_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67835.1"
                     /translation="MKRLLIIEDQLAIRQLIRITLELEDLEIHEAATGPEGLEAAQRL
                     QPQVVLLDLMMPGGLDGFQVCERIRGELGLSNTKVVLMTARDQPEDRARGMKAGADEY
                     LVKPFSPRELAGIVSRMT"
     gene            complement(4136..7369)
                     /locus_tag="PKV56_08195"
     CDS             complement(4136..7369)
                     /locus_tag="PKV56_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67836.1"
                     /translation="MKPSSAAAVQGAAPAARLNPFRLFYQLLASLILGMAVTAVVADK
                     WLPLTVGSQEWLVSLAAIALLSCSPLMLWQCIRAGMGTSRARGHMPFRVLGHTTRWTA
                     LTLAVGYSASLLGATWLMRAGEAQAWIFGLGGAVAASLVGLAVWLLGFGRARANALAD
                     EMTKDLRRLAKVVEHTSNAVVITDVHRRITWVNRGFTAISGYGLEEVIGASPGELLQC
                     PETDSVVVAAIRSALHHEEPFKGELLNRAKDGRLYWLAIEIQPLRDDDEVLQGFMAIE
                     TDITAVKEAQDRQSNAERLLRGAIDAVGEAFVLFDPEDRLVFANDQYRTLYSKSRNLI
                     LPGASFEAIIRGGVELGQFPQSMGREESWIQERLLQHRTGAVSVLQHLDDGRVARVAE
                     RRLADGHTVGFRIDVTDLTRATEAAQAATLAKSRFLANMSHEIRTPMNAIMGMTELLL
                     DGNLDADQRELVDTVRDSSESLLSLINDILDFSKIEAGEFHLEAIEFDLTACVSAAAR
                     TLASQAANKGLKLEWVIRPDVQTHVVGDPHRIRQVLLNLLSNAIKFTASGWVRVTVQR
                     QADAAPLSPLAELHVLVEDTGIGVTPDQAEKIFNAFTQADSSTTRKFGGTGLGLSICR
                     SIVERMNGKIWVESRIGQGSCFQFTACLAQPARVREAMRKQYQSTRTLDAALEPEEGR
                     VVIWRGEETTAQNLKTLLRSAGFLPQFVASAEEAQRAMDTGSGRPKRLLVCASALQAP
                     ARANASGALGKSAFDFGAVLVLDCELPDRMVTEFPSWLRWPASVSEVDAVLSAEAGFV
                     NSAVDAWSIENESKPRDLDSERPLKILLAEDHPVNQKLAVRLLRKLGYEVEVVDDGAA
                     AVDRCAEQAYDLVLMDVQMPVMGGFEATNLIRQHDRTVGRYTPIVAMTAHAMAGDRER
                     CLAVGMDDYLSKPITSAGLAKVIRDRARSTSENQADLQPRLSLTGLQAETHFDSEAAV
                     ARLGGDADLLAELVEDFLLRQEDDLKLIRSVFHGLNSGAALKGLHALRGAALSVGADK
                     LGADCGAVEESIRNERATDSTHLAGILETTYMSASAALRAFSLRSSG"
     gene            complement(7366..7827)
                     /locus_tag="PKV56_08200"
     CDS             complement(7366..7827)
                     /locus_tag="PKV56_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67837.1"
                     /translation="MDVNLAAPPCSPVLDLCFDATFEAVSQAGDRLAPLLNFHFGEER
                     GVQLRLGIIEAMTNVVEHGCAGREGGYLHLLAEAGMAQWCFTLTDNGRPIPRDAIMAA
                     DGSVFDFDPKAISELPEGGMGLSLIQMVFDQVDYKVSVMSNRLLLTAYLSA"
     gene            complement(7852..9012)
                     /locus_tag="PKV56_08205"
     CDS             complement(7852..9012)
                     /locus_tag="PKV56_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOX67838.1"
                     /translation="MKAMILAAGKGTRVRPLTHVMPKPMIPLIRKPIMEAIVDHLRAH
                     EFDEIYVNTSYLSQSIEDYFRDGDRFGVRMAFSYEGELRDGEFIDRPVGSAGGMRRIQ
                     DHCCFFDDTFAVLCGDALIDVDLSEVLKFHRQSGAMATIVTKTVPWEEVHRYGVVVSD
                     ASGRVERFQEKPKRAEAASNTINTGIYLFEPSVFEHIPAEGEYDIGSQLFPKLIASGA
                     HVAAISLPFQWVDIGCVADFWTATRMVLRGEVEGFRLPGREIAPGVRVGLGVHLAEGV
                     SCIKGPVYIGAGTTVGAGARIEGPTVIGANCIIEPGAHVRESILWDYTRVVQGSFVDE
                     RIVVAQRFVEPNGDSIDLREADLSWLLRDARSTASGSKAEELIQSVRTTIPL"
     gene            complement(9055..9381)
                     /locus_tag="PKV56_08210"
     CDS             complement(9055..9381)
                     /locus_tag="PKV56_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX67839.1"
                     /translation="MSILSMHSHGAITVFTLATPKFDASLVPEFKREFTELANPMPKD
                     LALDLSAVRFVDSSALGALVSVYKSAAGSRVRLVGVTAPVLGLLKLTRMDRVFEIVEA
                     LEPAAA"
     gene            complement(9397..10674)
                     /locus_tag="PKV56_08215"
     CDS             complement(9397..10674)
                     /locus_tag="PKV56_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX67840.1"
                     /translation="MSASLVATRPAVQTTPASTPRATVLVVDDQDMQRLIVKRTLGKM
                     GYQVVEAASAEEALAFMAEKPVDIVLSDWVMDGMDGTQLCQALRAQADRPYVYFILMS
                     SRDSREDLLAGLSAGADDFLRKPLDVDELSVRLRAGERVLDLQARLRARQRELELASQ
                     QIQEDVDAAAGFQRGLLPAHKHGQQEGAVQWLFMPSNAVSGDALNYFQVDDQHLVFYL
                     MDVSGHGVAAAMVAMNVAQYLNPEVEGCVFKQAQGRRVVLDAASAVAELNRRICAQET
                     ADKYLTCVYGILHLRTGRLQLVRAGHPLPMVVHADGTCDAIAEEGDMPVALFPDAEYT
                     NHELQLRPGSRLVVFSDGITECDDAVGEAYGEARLRRYFTSRAGQPLQQVTAEFGQEI
                     QAWRGFPTQTFQDDISLMVVEFAPRPERAANPP"
     gene            complement(10766..13198)
                     /locus_tag="PKV56_08220"
     CDS             complement(10766..13198)
                     /locus_tag="PKV56_08220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOX67841.1"
                     /translation="MSTQALTENSVREELRYGIGTDAAAADPYDVFRAISLSLRRRLL
                     DNLEATRSAQVQAEQKRVYYLSMEFLVGQSLINNALNLGSASGCGAALDALGHKLESV
                     AAHEPDAALGNGGLGRLAACFIDSMATLGIAGTGCGLRYDFGLFKQEIVDGQQRERPD
                     NWNAESSPWLLERHAESHQIPLYGHVEHVQAPNGEYLPMWMGWRSVIGVPYDMPVVGW
                     GGQTVNVLRLYSAKSSESFDMQIFNSGDFVKAVEQKVASETISKVLYPTDAVDAGREL
                     RLVQEYFLVACSLRDALRDFDRLGRPIAELPERVAIQMNDTHPSLAVAELMRILVDER
                     QLPWDDAWQLTVNTLAYTNHTLMPEALERWPVDLVQHVLPRHLQIIYEINHRFLQQVE
                     QRWPGNGAAKAELSIIEEGAVRKVRMANLSIVGSHSVNGVAALHTQLVKSHLVPRFHE
                     LWPAKFNNKTNGVTPRRWINQANPALASLITEAVGEGWLRDLDRLKGLERYAGDTGFL
                     DRLGAIKRQNKLRLVRHVAENQRIKVNPDTLFDVQCKRMHEYKRQLLNVLHVAHLYLR
                     IVDDGEQITPRTHLFSGKAAPGYWMAKEIIHLINAVASTVAQDRRAAGQLSCVFIPDY
                     RVSVAEWLIPSADVSEQISTAGLEASGTGNMKFAMNGALTVGTWDGANIEMAEHIGEE
                     NMYIFGLRAEEVEAWRRDRYEPFRVAEGSPALKRVLDAIAQGRFGRTPGQFAGIVNAL
                     MNWDTYFVLADFDAYANTQGRISSDFKQVADWQRKCLLNIARMGFFSSDRTIREYAKD
                     VWKLPVPADRAA"
     gene            13503..14186
                     /locus_tag="PKV56_08225"
     CDS             13503..14186
                     /locus_tag="PKV56_08225"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOX67842.1"
                     /translation="MDQRVKEPLWNLLGLLHKSHPWHGIDVGERFPQVLTAFIEIVPT
                     DTVKYEIDKITGHLMIDRPQSFSNVLPCLYGFVPRTLCADAVAQLAAQATGRADIEGD
                     EDPIDICILTENQISRGDIQAQAIPIGGMRMIDKGEADDKIIAVLKGDATYGAWRDIT
                     DVPPAVIARLKHYFLTYKRGPDDPAGACEIHEVYGADTARKVLQASQADYQARFPDIH
                     SLLNAALRG"
     gene            14209..16308
                     /gene="glgX"
                     /locus_tag="PKV56_08230"
     CDS             14209..16308
                     /gene="glgX"
                     /locus_tag="PKV56_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOX67843.1"
                     /translation="MKRDALPVRHAPQSSPWPLGPVTQATGTHFSVYAPDATAVEVCL
                     FDELGRQELQRHALPHRTHGVWHGHLDGVGAGRPYGLRAHGPWHPAAGLRHNAHKLLL
                     DPAAREVRVREDPQGLRRDHDGHGNPDRRDSAGAAWLAVTTAVLPLVDPSEHVRAPAP
                     DAVLYEAHVRALTQRHPQVPEALRGTYLGLVHPAVLQHLRDLGVTTLSLMPLMHWLDE
                     PRLTRMGLRNHWGYNPAAWFAPEPRLSTGKTSASARAECREMVRQLHAAGFEVVMDVV
                     LNHTAESDLEGPTLHLRGLSNRHSYHHHPHHAGQYENWAGCGNVVNLQDPWTLQLALD
                     ALRHWVQHMGVDGFRFDLAPVLGRVGHQGFRADAPLLAAMRQDPVLGRCRLIAEPWDI
                     GPGGYQLGRFDAPWQEWNDRFRDTARRFWLCGHTPRAALADVVAGSSAQFAGSGRAAC
                     TSINFITAHDGFTLRDLVSHNAKHNRANGEDNRDGHGDNHSWNCGAEGPTEDPQVRWR
                     RGRLSSALMATLAVSVGTPMLLAGDELGHSQGGNNNAYCQDNDTTWIQWAEADANLLA
                     QTQAWLALRRSRDVLRRSTWLEEHQVQWLTLDGHRLDDGAWHCPHTRTLGLCLGLGLG
                     DRSSSGDGAIAQPPLLLVFNAAHHDVQFQLPNLPQGQWHTLLDSTSIEPTPVTDGRIQ
                     VPAHAVVALEAQQESPL"
     gene            16305..17567
                     /gene="glgC"
                     /locus_tag="PKV56_08235"
     CDS             16305..17567
                     /gene="glgC"
                     /locus_tag="PKV56_08235"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOX67844.1"
                     /translation="MSKQLHSSQLVRRTLALVLAGGRGSRLQDLTHRRAKPAVFFGGK
                     FRIIDFALSNCVNSGIRRISVITQYKSHSLIRHLQRGWSFLRGELNETLEVLPASQRI
                     DEDSWYRGTADAVYQNLDIIRTKRPQFVLILAGDHVYKMDYSIMLEDHVQSGRACTVA
                     CIEVPRQEATAFGVMGADADRMITSFEEKPEHPKSMPGQPDRCLASMGIYIFNADYLY
                     ALLEADLQDPQSNHDFGKDIIPKVVREQQALAHPFSMSCVRSSDDAENYWRDVGTLDS
                     YWAANLDLAATLPQLDIYDNEWPMWTHQIQMPPAKFVPDHAGQPGVNVNTIVASGCVV
                     SGSLVRNSVLFPNVRVESFAQVQDSVLMPGTVVRSGCRIRKAVVDCGCDLPVGLVIGE
                     DAAEDARRFRRTEAGVVLVTAEMLSALG"
     gene            complement(17582..18061)
                     /locus_tag="PKV56_08240"
     CDS             complement(17582..18061)
                     /locus_tag="PKV56_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67845.1"
                     /translation="MNKAISCAVLAVSAALVGCGGGGGGADSGSGGTSGGGTAAPTTF
                     DQVVPGTTMSWDTSRSGAFAVTVTNAAGTSAANAAVRVFTMTMVNPDDGTPLDEAVAL
                     DLIDTAVTDGVGQASFDVRLPDSLTEVLVVVTWDDQKAIRRVTLASTVNSLAVSTAL"
     gene            complement(18084..19856)
                     /locus_tag="PKV56_08245"
     CDS             complement(18084..19856)
                     /locus_tag="PKV56_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HOX67846.1"
                     /translation="MTNRKEVTTYGGMGMSRRSKLIAFFALLSTLLCGYTVTGGLINW
                     QYFGTVSTGLPYVASTGSPLYAEFTRTLPAGLPALPTNLASKISFSLPEGRDIRLNSQ
                     GLIPDSDSKTNLQLSATADVWVTFLSEGAGYRNSVGYFTYDLANPPTSRDQVQEKIVF
                     VNASMPSPLDQASSTRQNTVYLGSFPAGTGVGFFIVSDGYSPTGRTLNGTRTGGVKDN
                     VSSKNVFYTLRALNPEATSTQNLNVHTVILRELSTSRPDYQLLVIGFEDINREQGGDH
                     DFNDVVLGIHVNPRTSLANLDSLAPLVSAADADTDGDGVKDLLDEYPNDATRAFSRSY
                     PSATGWGTLAYEDQWPARGDYDLNDVVMRYRSKEILNAQRNVAAVEMELGAVARGGLV
                     DSGFAVHFPGISASVVSSATLSVNGAAATPLAVEAGQSEAVFVVMPSSLAAMPSPTGC
                     SFANSQTGCPAVPIKNYKLTVNFSSARASSLFSPPYNPFIFRVGERGREVHLPGKPPT
                     ALATTSLFKTGVDNTTAGTTTTYMDAQRRPWALDIPTSWKHPEERTDVIRAYPAIVDW
                     ARTGGTSNKDWYTTPVTSLTYRAP"
     gene            complement(19920..20891)
                     /locus_tag="PKV56_08250"
     CDS             complement(19920..20891)
                     /locus_tag="PKV56_08250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX67847.1"
                     /translation="MYTTNMSLNPSPASAKDPQEDLVRRPRLLLVDDDINSILALRSA
                     LALDHQLIMARSGAEALQLLQGEPPDLILLDVDMPGMDGYEVCLRLKSAPILRDVPVV
                     FITAHRDSAAEVKGLDVGAADFISKPVEPRIVRARVRTQVMLKQQADMLRRLVYVDAL
                     TGVANRRQADERLNEYWRVSQRAGQPMAALLIDVDHFKQYNDRFGHATGDRCLRAVAL
                     ALKSVLLRGGDLLARYGGEEFVYLLPDTDLKGAESVAQRALQVVRDLRIPDEQGQHLS
                     EVTVSLGLASSEVDAVPEALDLLDLADRRLYQAKQAGRDRYVSPSAG"
     gene            complement(20873..22342)
                     /locus_tag="PKV56_08255"
     CDS             complement(20873..22342)
                     /locus_tag="PKV56_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67848.1"
                     /translation="MLLLDPSTTVGSAVAAIAHEQHWQLECASDLTHWSAQDRMPTVA
                     PPTYILCAEQWVTEARTAVAAHFGPGPAPVWLVLTKPRLAAMTSPVREVRESNGLHAL
                     SKPLTRQRLLAAMTHAESERAGQLPAAAPRASPMHKVLMGLRILLVEDNPDNQFVATE
                     LLRAAGAHVSLACDGQEAVERLAHAASLPDVVLMDMQMPMMGGLEATRRLRAMPALQS
                     LPIVAVTANASAADRQSCLDAGMNEHVGKPFDLAHLVMVILDLTRHQASLPAPPPEGS
                     VLPMALAEAHGVDLAGALKRLGHDEELYLEMLDRLIQDLHKLPDQVLQDVSRANSLQA
                     SRLFHKLKGSAGTLGVQELYEASAAAERELAAEPSGPAAMVLAERAVAVMLSSLPRLE
                     ALQSRWSEGTWVREGREEPLDPLQAANLQELRALLERSDLGALDVGSHLGLSAREEVQ
                     PWGSVLDKSIRQLNFPAALEALSGLQAHLGRSGTCTPRI"
     gene            22495..23472
                     /locus_tag="PKV56_08260"
     CDS             22495..23472
                     /locus_tag="PKV56_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOX67849.1"
                     /translation="MKQQTLAMAADQSASFERYRKPTRRDEFLATMNAIVPWAQLCDV
                     VQPYYPKGVGGRPPIGLERMLRIHFIQHWFNLADEACEEALYDSASLRRFVGIDLGRE
                     PVPDATTMLKFRRLLNDNKLGEQLFAKVGEVLQQGGFKLKTGTIVDATIIGAPSSTKN
                     ADNARDPEMHQTRKGNQWYFGMKMHIGVDSQSGLAHSAVVTAANVHDKHPLPELLHGN
                     ERRVYGDSAYASQKDLIAGKAPRAKDFTNERVRNRQGEVDEAARAKNRNKSRIRARVE
                     HVFCVVKRLWGFSKVRYRGLAKNATRAFTALALANIYLARGRLMAQVRP"
     gene            complement(23553..>24045)
                     /locus_tag="PKV56_08265"
     CDS             complement(23553..>24045)
                     /locus_tag="PKV56_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67850.1"
                     /translation="INVQLAPMDLEAMLTDLGEVFGLQAAEKSLELVVCTEPGMPEVV
                     VSDARRLRQALNCLLGNAVKFTQQGEVKLSLSVTGGAADMQDEVALHLVVEDTGPGIP
                     TSELESIFQPFAQGAIGAVRRQGGAGLGLSLAQGIVQALGGSIRDGLKKWPHREMRVR
                     YVS"
BASE COUNT         4372 a         7840 c         7511 g         4322 t
ORIGIN      
        1 tccatcaggc tgtcgtgcgt gccggtgtcc agccaggcgt agccgcggcc catgatctcc
       61 acgttgagct ggttctggtg caggtactgc gcgttgacgg cggtgatctc cagttcgccg
      121 cgcgcgctgg gcttgatgga cgccgcgatg tcgcagacct gctcgtcgta gaagtacagg
      181 ccggtcaccg cgtagttgct cttcggcgct ttgggctttt cctcgatgct cagggcacgg
      241 cgctccgcat cgaactcgac cacgccgtag cgctccgggt cgtgcacgtg gtacgcgaaa
      301 acggtggagc cgctgctgcg gccgtcggcc cgcgccagca ggctggcgaa gtcatgcccg
      361 tagtagatgt tgtcgcccag caccagggcg ctcggcccac cgcccacgaa ctgacggccc
      421 aggatgaagg cctgcgccag gccgtccggg ctgggctgca cgcagtagct gaggttcatg
      481 ccccaggcgc tgccgtcgcc cagcagggac tggaagcgcg gcgtgtcctg cggggtgctg
      541 atcaccaaca cgtcgcgcat gccggccagc atcagcgtgg acagcgggta gtacaccatg
      601 ggcttgtcgt acaccggcag cagctgcttg ctgatggcca gcgtggcggg gtgcagccgg
      661 gtgccggagc ccccggccag gatgatgcct ttgcggttca tgtcgttttc tcctgcgggt
      721 gctgggtcag cccaggactt cggtcagcat gcgctgcacg ccttgttgcc agtgcggcag
      781 ggtcacgcca aaggccgact ggagcttgcg cgtacccagt cgagaattca gcggccgctg
      841 cgccggcgtc gggtaggccg acgtggggat ggccgcgatc ttgtccggcg ccacctggat
      901 ggcctggccg cgggcgcgcg cccattcgat gacgaactgc gcgtagccgt gccagctggt
      961 ctcgcctgcg gcggcacagt ggtaggtgcc gcccagcgat gcgtcggccc gcaggcgggg
     1021 cagggcgaga gcggtcatgt cggccagcag gtcggcccct gtgggcgcgc ccacctggtc
     1081 ggcaatgacg ttcagcgcgt cccgctccgc cgccagccgc agcatggtgc gcgcgaagtt
     1141 gccgccgcgc gccgcgtaga cccagctggt gcgcagaatg aggtgcctgc agccactggc
     1201 gcggatgcgt tcttccgctt ccagcttggt gcggccgtat acgctgagcg gcgcggtggg
     1261 tgcgtcttca gttcgcggct ggctgccgct gccgtcgaag acatagtcgg tgctgtagtg
     1321 caccaggatc gcgccaaggg ctgctgcctc gcgggccagc acagcggggc cggtggcgtt
     1381 gatgcggcgg gccaggtcgg gttcggactc agccttgtcc accgcggtgt gcgcggcggc
     1441 attgacgatg gcgtctggcc gcacacgctg caccagcggt gccagcgatt cgggttccga
     1501 gaaatcggcc ttcaggtcac ccgctgaatc gaagtcgcag gccaccacct cgcccagcag
     1561 cgacagcgaa cgttgcagtt cccagccgac ctggccgccc ttgcccagca aaaggatctt
     1621 catgcgccgt actgctggtt gacccagtcg cgatagccgc cgctttgcac gttggccacc
     1681 cagtccgcat gggccaggta ccactgcacg gtcttgcgga tgccagtgtc gaaggtttcg
     1741 gcagggcgcc agccgagttc gcgttcgatc ttgcgcgcgt cgatggcgta ccgccgatca
     1801 tgaccggggc ggtccttcac gtaggtgatg agtcgcgtgt agctgccttc cgggctgggc
     1861 cgaagttcgt ccagcaacgc gcacaccgtc ttcacgatct cgatgttggg cttctcgttc
     1921 cagccgccca cgttgtacgt ttcgcccaga cggccaccgg ccagcacggc gcggatggcg
     1981 ctgcagtggt cccccacgta gagccagtcg cgcacctgct ggccatcgcc gtacaccggc
     2041 agcggtttgc cagcgagggc gttgacgatc atcagcggaa tcagtttttc cgggaagtga
     2101 tagggcccgt agttgttgct gcagttggtg gtgaccaccg gtagcccgta ggtgtggtgc
     2161 caggcacgca ccaggtggtc gctggccgcc ttgctggcgc tgtagggact gttgggctcg
     2221 aaggggtggt tctcggtgaa ggcgggggct gtcggcgaca ggctgccgta gacctcgtcc
     2281 gtgctcacgt ggtggaagcg gaaggtcgcc ttggcggcat cgtccagacc gctccaatac
     2341 ccccgtgcag cctccagcaa ggtgaaggtg ccgtcgacgt tggtcttcat gaaagcgccc
     2401 gggccgtgga tgctgcggtc cacatggctc tctgcggcga agtggacgat ggcgcggggc
     2461 cgatgcgtgg ccaacaggct gtccaccagt gcgcggtcgc agatgtcgcc cttgacgaag
     2521 gtgtgacggg tgtcgccctg cagggactgc aggttctgca ggttgccggc gtaggtcagt
     2581 gcgtccaggt tgacgacggc ctcgttcgac tgggccagcc agtccagcat gaagttgctg
     2641 ccgatgaagc cggcaccgcc ggtgacgagg atggtcactt caggactcct tgaaaagttt
     2701 ctgcatttcc gtgcatcagc acggcatggc ctctcgagat ttcgcaagga ttcaaccggc
     2761 caggatgatg atgcgccttc agataaaggg tccggaggac cttttcggcc gttgagattt
     2821 cttccgtttg tcccagctca caaaaagccc ttccaaagaa ctctgcgaca ccgttagttg
     2881 tcttcagaaa aagggagtta gcgccccttt tttgagtcag ggccgcttca acccattgtc
     2941 atcagtaatg gcgaacacag aagctgcagc aatgcagtgc tgacaataga tcattaactc
     3001 aactcatttc tatcccacaa gcgaagcgag aaaatttaat gggttttcgg tctgcttcta
     3061 gaggcctttc gagggcttgc agaatcgtgc gcgaattctg gaataaatac acagaagtgc
     3121 atacatttgg atcttcgaca ctataagcaa ggtggcgatc gacgagagta gctccgaagt
     3181 agcttgggct cagtgatcca gtcacaataa actcgaatca agcggcatag cgcacacggt
     3241 gcgcgcggcc agttcgatgg cgtagcggtg gggaacagct cgtccaaggc ctgcccccgc
     3301 gtccacagct cgtaggcaac cttcaactga tcgggtgtgt cgttacggat catgcgctcg
     3361 acttcgcatt cctgctctga agtgacggtc tgaccgctgc ccaccggcct gcccgcagcc
     3421 ggcggcatag ccctttacaa ccgcgatcac cgtcgtgcgc cacatcttga aatgcaccgc
     3481 cgtgtccttc aggtgcgagc cgtctagtcc caccttcacc gcacgcagac ggcgcttact
     3541 gagcgggtcc agcggcggcg ttcaggactt aaccttgcct atgatcgacc cgactctacg
     3601 acatgcacta cgctcaactg tttaatgtcg gttcaatatt caagtcatcc ggctgacgat
     3661 gcctgccagt tcccgcgggc tgaagggctt gactaagtac tcatccgcac ccgccttcat
     3721 gccgcgggcg cgatcttcgg gctggtcacg tgccgtcatt aagaccacct ttgtgttgct
     3781 caggcctaac tcccctcgga tgcgctcaca gacttggaag ccatccaagc cgccgggcat
     3841 cattaagtcg agcagcacca cttgcggctg gagtcgctgc gcagcttcta gaccttcggg
     3901 gccagttgcg gcttcgtgga tttccagatc ctccaactcc agtgtaatgc gtatgagttg
     3961 gcggatggcc aattggtctt caataatgag gaggcgtttc atgacatcta ctccaagatc
     4021 ctagtttgaa gacggcgatg atattgactc taccggcggg caccccgact tggctgaagt
     4081 gaagcgggcc gattgcatat ctctttgggg accagtttca gtttagcgcc gaaaactacc
     4141 cgctggatcg aagcgaaaac gcccgcagag ctgcgctcgc tgacatgtaa gtcgtctcta
     4201 agatcccggc aagatgcgtc gaatcagtag ctcgctcgtt gcgaatggat tcttcaaccg
     4261 caccacaatc agcaccaagc ttgtctgcac ccacggaaag cgctgcacca cggagagcgt
     4321 gcagaccttt caacgctgct cccgaattca aaccatgaaa cacagatcga atcagcttca
     4381 aatcgtcttc ctggcgcagc agaaagtcct cgaccagttc tgccaacaaa tccgcgtcgc
     4441 cacctaatcg agccactgca gcttcgctgt caaaatgcgt ttcagcctgc aagccggtca
     4501 gactcagccg cggctgcaga tcagcttgat tttctgacgt cgaacgagcg cggtcacgga
     4561 tgactttcgc caatccagca gacgtgatgg gcttgctcaa gtagtcatcc atgcccactg
     4621 caaggcagcg ctcgcggtct ccagccattg cgtgggctgt cattgccaca ataggtgtgt
     4681 accgcccgac cgttcggtca tgctgacgaa tcaagttcgt tgcttcgaag ccacccatca
     4741 cgggcatctg cacatccatc agcaccaggt cataggcctg ctcggcgcag cggtctaccg
     4801 ctgccgcgcc gtcatccact acttcaactt cgtaaccgag cttgcggagc aggcgcacag
     4861 ccagtttctg gttcactggg tggtcctctg ccagcaagat cttcaatggc cgttccgagt
     4921 ccaggtccct tggcttggac tcgttttcta tcgaccaagc atccactgca ctgttcacga
     4981 agcccgcttc agcagacagg actgcatcca cttcgctaac agatgcgggc cagcgcaacc
     5041 agctcgggaa ttcggtcacc atcctgtctg gaagctcgca gtccagcacc agtacggcac
     5101 caaagtcaaa ggcggacttg cccagcgccc cacttgcatt ggcccgagct ggcgcctgca
     5161 gtgctgacgc gcaaacaagc agtcgctttg gccggccaga gcccgtgtcc atggctcgct
     5221 gcgcctcttc agcgcttgca acgaactgtg gcaaaaagcc ggcgcttcgc agcaatgtct
     5281 ttaggttctg tgcggtggtc tcctcgccgc gccagatcac gactcgaccc tcttccggct
     5341 caagcgcagc gtctagagtc cgcgtggact ggtactgctt gcgcatggct tcccgaaccc
     5401 gagccggttg tgccaagcag gctgtgaatt gaaagcagct tccctgaccg atcctgcttt
     5461 ccacccagat cttgccattc atgcgttcaa cgatgcttcg acaaattgac agtccaagcc
     5521 ctgtgccccc gaacttgcgt gtggttgaac tgtctgcctg cgtgaacgca ttgaagatct
     5581 tctcggcctg atccggcgtt actccgatgc cggtgtcttc gaccagcaca tgcaactccg
     5641 ccaacgggct gagcggagcg gcatccgcct ggcgttgaac ggtgaccctg acccagccac
     5701 tggccgtgaa cttgatggcg ttgctcagaa ggttcagcaa cacttgacga atccggtgcg
     5761 ggtcacccac cacatgggtc tggacatccg gacgtattac ccattccagc ttcaagccct
     5821 tgttcgctgc ctgactggcg agggttcgcg cggcggcgct aacgcacgcg gtcaagtcga
     5881 actcaatcgc ctccagatga aactcgccgg cttcgatctt tgaaaagtca agaatatcgt
     5941 tgatcaagct gagcagtgac tctgacgaat cacgcaccgt gtccacaagc tctcgttggt
     6001 ccgcatccaa attgccgtcc agtagcaatt ccgtcatacc catgatcgca ttcatgggcg
     6061 tgcgaatttc gtggctcatg ttcgcgagga agcgactctt ggccaacgtc gccgcttgtg
     6121 cggcctctgt ggcacgggtc aggtctgtca cgtctatgcg gaaccctaca gtgtggccat
     6181 cggctaggcg ccgctccgcg acgcgggcaa cgcgcccatc gtctagatgc tgcagaactg
     6241 acacagcacc tgtccgatgc tgcaacaagc gctcctgaat ccaagattct tctcgcccca
     6301 tagattgggg aaactgaccc agttccacac caccgcgaat gatcgcctcg aagctggcac
     6361 cgggaagtat caagttgcgg gacttgctgt acagcgtccg gtactggtcg ttggcaaata
     6421 caaggcggtc ttcagggtca aagagcacaa aggcctcgcc gacggcatca atggcaccac
     6481 gcagcaggcg ttctgcattc gactgccggt cctgggcttc cttgaccgca gtgatgtcgg
     6541 tctcaatggc catgaatccc tgcagcacct cgtcgtcgtc acgcagcggc tgaatttcta
     6601 tggcgagcca gtaaagccgc ccgtccttgg cacggttgag aagttcaccc ttgaagggct
     6661 cctcatggtg caaggccgag cgaatggcgg ccacgaccac tgaatcagtc tcggggcatt
     6721 gcagcaactc gcctggagat gccccaatca cctcttccaa gccgtaaccg gagatcgcag
     6781 taaacccacg gttgacccac gttattctgc ggtgcacatc ggtaataaca acagcgttgg
     6841 aggtgtgctc gacaaccttg gccaaccgcc gaagatcctt cgtcatctcg tcagccaaag
     6901 cgttggcacg agctctcccg aagcccagca gccacacagc caaacccacc aacgaagcgg
     6961 cgactgcccc acccaatccg aagatccaag cctgagcctc accggcgcgc atcagccatg
     7021 tcgcgcccag cagggaggct gagtaaccca cggccagagt caaggccgtc cagcgagtgg
     7081 tatggcccag aacacgaaat ggcatgtggc cacgtgcacg agaagttccc atgcctgccc
     7141 ggatgcactg ccacaacatc agtggggagc aggacagcag tgcaatggcc gctaaactca
     7201 ccaaccactc ttgggagccc acggtcaatg gcaaccactt gtcagcgacg accgcggtga
     7261 cggccattcc aagtatcaag ctcgcaagta gctggtagaa caggcgaaaa gggttcaatc
     7321 gagcggccgg agccgcccct tggactgccg cggcgctgct cggcttcatg cgctcaggta
     7381 tgcggtcagc agcaatcgat tgctcatgac cgaaactttg tagtccactt ggtcgaagac
     7441 catttgaatc aatgaaaggc ccatcccgcc ctctggcagc tccgaaatgg ctttggggtc
     7501 gaagtcgaac accgagccat cagcggccat gatcgcgtcg cgagggatgg gccgcccgtt
     7561 gtcagttagt gtgaagcacc actgggccat cccagcttcg gccagcaggt ggaggtaacc
     7621 gccctcccgc cccgcgcatc cgtgctcaac gacgttggtc atggcctcaa tgatgcccag
     7681 gcgcagctgc acgccgcgct cttctccgaa gtggaaattc agaagtggcg ccaggcggtc
     7741 accggcttgg gacactgctt caaacgttgc atcgaagcaa aggtccagca ccgggctgca
     7801 cggcggggct gcaaggttga cgtccataag agcacttcca gagctcgttg atcaaagcgg
     7861 aattgtggtg cgaacgctct gaatcagctc ttcagctttc gatccgctgg cagtgctgcg
     7921 agcgtcacgg aggagccagc tcaggtcggc ttcgcgcagg tcaatagagt cgccgttggg
     7981 ttccacaaag cgctgggcga ccacgatgcg ttcgtccacg aaactgccct gaaccacccg
     8041 tgtgtagtcc cagaggatgg attcgcgcac atgggcaccg ggctcgatga tgcagttggc
     8101 accaatgacc gtcggccctt cgatgcgcgc accagcgccc accgtcgtgc cggcgccgat
     8161 gtagacgggg cccttgatgc aggaaacccc ctcggccagg tgcacgccca gccccacccg
     8221 cacgcctggc gcaatttcgc gccctggcag acgaaagcct tcgacctcac cgcgcagcac
     8281 catgcgggtg gcggtccaga agtcggccac gcagccgatg tcgacccact ggaacggcag
     8341 gctgatggcg gccacgtgcg ccccggaggc gatgagcttg gggaacagct gcgagccgat
     8401 gtcgtattcg ccttcggccg ggatgtgctc gaaaaccgac ggctcaaaga ggtagatgcc
     8461 agtgttgatg gtgttggaag cagcctcagc acgcttgggc ttttcctgga agcgctcaac
     8521 gcgacctgaa gcatcgctaa ccaccacgcc gtagcggtgc acctcttccc agggcaccgt
     8581 cttggtcacg atggtggcca tggccccgct ctggcgatgg aacttgagga cctcgctcag
     8641 gtcgacgtcg atcagcgcgt cgccacacag cacagcaaag gtgtcgtcaa agaaacagca
     8701 gtggtcctgg atgcgccgca tgccgccggc cgagcccaca ggccggtcga tgaactcgcc
     8761 gtcgcgcaac tcgccttcgt aggaaaaggc catgcgcacg ccgaatcgat caccgtcgcg
     8821 aaaatagtcc tctatagact gcgacaggta gctggtgttg acgtagatct catcaaattc
     8881 gtgcgcacgc aagtggtcca cgatggcttc catgatcggc ttgcggatca ggggaatcat
     8941 cggcttgggc atgacgtgcg tcagcggtct gacacgcgtg cccttgcctg ctgccaggat
     9001 catggctttc atgtgcggtc ctttgttgtc ctgcggtttt gaaggctcag gcgttcaggc
     9061 cgcggccggt tccagtgctt cgacgatttc aaagacacgg tccatgcgcg tgagcttgag
     9121 caggcccagc acaggcgcgg tcacgcccac caggcgcacc cggcttccgg cagcggactt
     9181 gtagaccgac accagggcgc ccagcgcact ggagtccacg aagcgcacgg cgctcaggtc
     9241 caaggccaag tccttgggca tgggattggc cagctcggtg aactcccgct tgaactcggg
     9301 caccagggag gcgtcgaact tgggcgtggc cagggtgaac acggtgatgg ctccgtggct
     9361 gtgcatgctc agaattgaca tcgtgggttc ctctcttcaa ggggggttgg cggcccgctc
     9421 gggccggggg gcgaactcga cgaccatcag tgagatgtcg tcttggaagg tctgggtcgg
     9481 gaagccccgc caggcctgaa tttcctggcc gaattcggcc gtgacttgct gcaatggctg
     9541 gcctgcacgg gaagtgaagt agcgcctcag ccgtgcttcg ccgtaggcct cacccacggc
     9601 gtcgtcgcac tcggtgatgc cgtcagaaaa gaccaccagc cgcgagcccg ggcgaagttg
     9661 caattcgtgg ttggtgtact ccgcgtccgg gaacagagcg accggcatgt cgccttcttc
     9721 ggcgatcgca tcgcatgttc cgtcggcgtg caccaccatg ggcagcgggt ggccggcccg
     9781 caccagctgc agcctgcccg ttcgaagatg gaggatgccg tagacgcagg tcaggtactt
     9841 gtcggccgtc tcctgtgcgc agatgcgccg gttcagttcg gccacagccg aagcggcatc
     9901 cagcaccacg cggcgtccct gtgcctgctt gaacacacag ccctccacct cggggttgag
     9961 gtattgcgcg acgttcatgg ccaccatggc agcggccacg ccgtggccgg acacgtccat
    10021 caggtagaag accagatgct ggtcatcgac ttggaaatag ttcagcgcgt cgcccgagac
    10081 cgcattggaa ggcatgaaca gccactgaac agcgccttcc tgctgaccgt gcttgtgcgc
    10141 gggcaacaag ccgcgctgga atccggcagc ggcatccacg tcttcttgga tctgctgtga
    10201 cgccagctcc agttcgcgct gccgcgcgcg cagccgggcc tgcaggtcca gcacccgctc
    10261 gccggcgcgc aagcgaaccg acagttcgtc cacgtccagc ggcttgcgca ggaagtcgtc
    10321 cgcaccggcg ctcaagcctg ccaggaggtc ttcgcggctg tcgcgagagg acatcaggat
    10381 gaaatacacg taaggccggt cagcctgcgc acgcagggcc tggcacagct gggtgccgtc
    10441 catgccgtcc atgacccagt cgctcagcac gatgtccacg ggcttctcag ccatgaaggc
    10501 cagcgcctct tcagcactgg cggcctccac gacctggtag cccatcttgc ccagggtccg
    10561 cttgacgatc aggcgctgca tgtcctggtc gtcgaccacc aggacagtgg ccctgggggt
    10621 agaagcgggc gtggtctgca ctgcaggccg ggtggcaacg agcgaagcgg acaaggcgag
    10681 ttcctgataa cgattggggc tttttcggcg cacggcgccc cgacttgagc cgaaccggtc
    10741 tccaggcgtt gcgcgcgaat cagcctcaag ccgcccggtc cgcaggcacc ggcagcttcc
    10801 acacgtcctt ggcgtactcc cggatggtgc ggtcgctgct gaagaagccc atccgtgcaa
    10861 tgttcaacaa gcacttgcgc tgccaatccg ccacctgctt gaagtcggaa gagatccggc
    10921 cctgggtgtt ggcataagcg tcgaagtccg ccagcacgaa ataggtgtcc cagttcatca
    10981 gcgcgttgac aatgccggca aactgccctg gcgtgcgccc gaatcggccc tgtgcaatgg
    11041 cgtccagcac gcgcttgagc gcgggcgaac cctcggccac gcggaagggt tcgtagcggt
    11101 cgcgtcgcca ggcttccacc tcttcagcgc gcagcccgaa gatgtacatg ttctcctcgc
    11161 cgatgtgttc ggccatctcg atgttggccc cgtcccacgt gcccaccgtc agcgcgccgt
    11221 tcatggcgaa cttcatgttg cccgtaccgc ttgcttccag gcccgccgtg gaaatctgct
    11281 cgctcacgtc agctgatgga atcagccact cggccactga cacacggtag tcgggaatga
    11341 agacgcagct cagttgcccg gcggcgcggc ggtcctgggc cacggtgctg gccaccgcat
    11401 tgatcaagtg gatgatctcc ttggccatcc agtagcccgg tgcggccttg ccggagaaca
    11461 ggtgtgttct gggcgtgatc tgctcaccgt cgtccacgat ccgcaggtac aggtgggcca
    11521 cgtgcaggac gttcagcagt tgccgcttgt attcatgcat gcgcttgcac tgcacatcga
    11581 acagggtgtc ggggttgacc ttgatgcgct ggttctcggc cacatgccgc accaggcgca
    11641 gcttgttctg ccgcttgatt gcacccagcc ggtccaggaa gccggtgtcg ccggcatacc
    11701 gctccaggcc cttgagccgg tccaggtcac gcagccagcc ttcacccacc gcttcggtga
    11761 tgagtgatgc gagcgccggg ttggcctggt tgatccagcg gcgtggtgtt acgccgttgg
    11821 tcttgttgtt gaacttggcc ggccagagtt cgtggaaccg cggcaccagg tggctcttca
    11881 ccagctgcgt atgcagcgca gcgacaccat tgaccgagtg gctgccgacg atgctcaggt
    11941 tggccatgcg caccttgcgt accgcgcctt cctcaatgat cgacagttca gccttggccg
    12001 cgccattgcc gggccagcgc tgctccacct gctgcaggaa ccggtggttg atctcgtaga
    12061 tgatctgcag atgcctgggc agcacgtgct gcaccaggtc caccggccaa cgctccagcg
    12121 cctcgggcat cagggtgtgg ttggtgtagg ccagcgtgtt caccgtcagc tgccaggcgt
    12181 cgtcccaggg cagctgccgt tcgtccacca ggatgcgcat cagttccgcc accgccagcg
    12241 aagggtgcgt gtcgttcatc tggatggcga cgcgctctgg caattcagcg atgggccgac
    12301 ccagtcggtc aaagtcccgc agcgcgtcgc gcagtgaaca ggccaccagg aagtactcct
    12361 gcaccaggcg cagttcacgc cctgcgtcca ccgcatccgt ggggtacagc accttggaaa
    12421 tggtctctga cgccaccttc tgctccacgg ccttgacgaa gtcgccggag ttgaagatct
    12481 gcatgtcaaa gctctcggag ctcttggcgg aatacaggcg caggacattc accgtctggc
    12541 cgccccagcc caccaccggc atgtcgtaag gcaccccgat cacgctgcgc cagcccatcc
    12601 acatgggcag gtattcacca ttgggcgcct gcacgtgttc cacatgcccg tacagcggga
    12661 tctggtgcga ctcggcgtga cgttccagca gccaaggcga gctttcggcg ttccagttgt
    12721 cggggcgctc gcgctgctgg ccatcaacga tttcctgctt gaacagcccg aagtcgtagc
    12781 gcaggccgca accggttcct gcaattccca gcgtggccat ggagtcgatg aagcaggccg
    12841 ccaggcggcc caggccgcca ttgcccaagg cggcgtccgg ctcgtgtgcg gcgaccgact
    12901 ccagcttgtg ccccagcgcg tccagtgcag caccacaacc cgatgcgctg cccaggttca
    12961 gtgcattgtt gataagcgac tgccccacca ggaattccat ggagaggtag tacacccgct
    13021 tctgttcggc ctgcacctgc gcgctgcgcg tggcttccag gttgtccagc aaacggcggc
    13081 gaaggctgag gctaatcgcg cggaacacgt cgtaggggtc ggctgcagcg gcgtcagtgc
    13141 ctatgccata gcggagttcc tcgcggacgc tgttctcggt cagggcctgt gtgctcatgg
    13201 tgtgtccttg gtattgcgaa cctgcatcag ccatgagtcc acgctcaagc ccgcgttgca
    13261 ggggtcagcc cgcattgaac cgatttgggg cgcccggcaa aggccgagta gcgggcctga
    13321 accgactcaa ttcgtttgat cggcgcgggt gtcagccagc acggagatat ggcggtgctt
    13381 cagacagttg ttccgggtca agctgcagag cgatgctggc tagagttcgg tcccatgccc
    13441 gcgcggcatg agcgcgcggc ttgcatctgt agtctgcaca ccatctgaag gagcaccccg
    13501 tcatggatca gcgtgtcaaa gaacccttgt ggaacctgtt aggcctgctg cacaagtcgc
    13561 acccctggca cggcatcgac gtgggtgagc gttttccaca ggtgctgacg gccttcatcg
    13621 agatcgtgcc cactgacacc gtgaagtacg agatcgacaa gatcaccggc cacctcatga
    13681 tcgaccggcc gcagagcttt tccaatgtgc tgccttgctt gtacggcttt gtgccacgca
    13741 ccctgtgcgc agacgctgtg gcccagctgg ccgcccaggc cacgggccgt gccgacatcg
    13801 aaggggacga ggacccgatc gacatctgca tcctcacgga aaaccagatc tcgcgcggcg
    13861 acatccaggc ccaggccatt cccattggtg gcatgcgcat gatcgacaag ggcgaagccg
    13921 acgacaagat cattgccgtg ctcaagggcg acgccaccta cggcgcctgg cgcgacatca
    13981 ccgatgtacc gcccgccgtc atcgcgcgcc tgaagcacta cttcctgacc tacaagcgcg
    14041 ggccggacga ccctgccggc gcgtgcgaga tccacgaggt ctatggcgcc gacacggcgc
    14101 gcaaggtgct gcaggccagc caggcggact accaggcgcg cttcccggac atccacagcc
    14161 tgctcaacgc cgccctgcgc ggttgagcac cagccggcac aggcctgcat gaagcgcgac
    14221 gccctgcccg tacggcacgc cccgcaaagt tcgccctggc cgctgggccc ggtcacccaa
    14281 gccacaggca cgcatttcag tgtctacgcc ccggacgcca cggcggtgga ggtgtgcctc
    14341 tttgacgagt tggggcggca ggaactgcaa cggcacgccc tgccccaccg cacccacggg
    14401 gtctggcacg gccacctgga cggtgtcggc gccggcaggc cctatggctt gcgtgcccac
    14461 ggaccctggc accccgccgc cggcctgcgc cacaacgcac acaagctgct gctcgacccc
    14521 gcggcacgcg aagtgagggt ccgcgaagac ccgcagggcc tcaggcgcga ccacgacggc
    14581 cacggcaacc ccgaccgccg cgacagcgcc ggcgcggcct ggctggccgt gaccactgcg
    14641 gttctacccc tggtggaccc gtcggaacat gtgcgggcgc cggcacccga tgcggtgctg
    14701 tacgaagcgc atgtgcgcgc actcacgcag cggcacccgc aggtgccaga ggctcttcgc
    14761 ggcacctacc tgggcctggt tcacccggca gtgctgcagc acctgcgcga cctgggcgtg
    14821 acgacattga gtctcatgcc gctgatgcac tggctggacg aacctcgcct cacccgcatg
    14881 ggcctgcgca accattgggg ctacaacccc gcggcctggt ttgcgcccga gccgcgcctg
    14941 tcgaccggca agaccagcgc cagtgcgcgg gccgagtgcc gtgaaatggt gcgccagctg
    15001 catgccgccg ggtttgaagt ggtcatggac gtggtgctga accacaccgc cgagagcgac
    15061 ctggaaggcc ccacgctgca cctgcgcggc ctgtccaacc gccacagcta ccaccaccac
    15121 ccgcaccacg ccggccagta cgaaaactgg gcgggctgcg gcaacgtggt caatctgcaa
    15181 gacccttgga cgctgcaatt ggcgctggac gccctgcgcc actgggtgca gcacatgggg
    15241 gtggacggct tccggtttga cctagcaccc gtgctcgggc gcgtgggcca ccagggcttc
    15301 agggccgacg cgccgctgct ggccgccatg cgccaagacc cggtgctggg ccgctgccgc
    15361 ctcattgccg agccctggga catcgggccc ggcggctacc agctgggccg cttcgacgcg
    15421 ccctggcaag agtggaacga ccgcttccgc gacaccgcgc ggcgcttctg gctgtgcggc
    15481 cacacgccac gtgctgcgct ggccgatgtg gtggcgggct ccagcgccca gttcgcgggc
    15541 tccggccgcg ccgcctgcac cagcatcaac ttcatcaccg cgcacgacgg cttcaccctg
    15601 cgcgacctgg tcagccacaa cgccaagcac aaccgggcca atggcgaaga caaccgcgac
    15661 ggccacggcg acaaccacag ctggaactgc ggtgccgaag gccccacgga agacccgcag
    15721 gtgcggtggc gccgtgggcg cctgtcaagc gccctgatgg caacactggc cgtctccgtc
    15781 ggcacaccca tgctgctggc cggcgacgag ctgggccaca gccagggtgg caacaacaac
    15841 gcctattgtc aggacaacga caccacctgg atccagtggg ctgaggcgga tgcgaacctc
    15901 ctggcacaga cccaggcctg gctggcgctt cggcgcagcc gtgacgtgct tcgccgaagc
    15961 acctggctgg aagagcacca ggtgcagtgg ctgacgcttg acggccaccg gctggacgac
    16021 ggcgcctggc attgcccgca cacacgaacc ttggggcttt gcctgggctt gggtttgggc
    16081 gaccgcagca gcagcggcga cggcgccatc gcgcagcctc ccctgctcct agtctttaac
    16141 gccgcacacc acgacgtgca gttccagctg cccaacctgc cccagggcca gtggcacacc
    16201 ttgctggaca gcacttccat agaacccacg cccgtcactg atggccggat tcaagtcccg
    16261 gcccatgccg tggtggcgct ggaagcccag caggagagcc cactttgagc aagcagttgc
    16321 acagcagcca gttggtgcgc agaaccctgg ccctggtcct ggccggcggg cgcggctcca
    16381 ggctccagga cctgacccac cgccgggcca agcctgcggt cttctttggc ggcaagttcc
    16441 gcatcatcga ctttgcgctg tccaactgcg tcaactccgg catccggcgc atttccgtca
    16501 tcacgcagta caagtcgcac tcgctcatcc ggcacctgca gcgcgggtgg agcttcctgc
    16561 gcggggaact caacgagacc ctggaagtgc tgcctgcgtc ccagcgcatc gacgaagaca
    16621 gctggtaccg cggcacagcc gacgccgtgt accagaatct ggacatcatc cgcaccaagc
    16681 ggccgcagtt cgtgctgatc ctggccggcg accacgtcta caagatggac tactccatca
    16741 tgctggaaga ccacgtgcag agtggccgcg cctgcacggt ggcttgcatc gaggtgcctc
    16801 ggcaagaggc gacggccttc ggggtgatgg gtgccgatgc cgaccggatg atcaccagct
    16861 tcgaggaaaa gccagagcac cccaagagca tgccgggcca gcccgaccgc tgcctggcca
    16921 gcatgggcat ctacatcttc aacgccgact acctgtacgc gttgctcgag gccgacctgc
    16981 aggacccgca gtcaaaccac gacttcggca aggacatcat tcccaaggtc gtgcgggaac
    17041 agcaggcgct ggcgcatccg ttttcgatgt cctgcgtgcg ttcttcagac gacgccgaaa
    17101 actactggcg cgacgtgggc acgctggaca gctactgggc tgccaacctg gacctggcgg
    17161 ccacgctgcc gcagctggac atctacgaca acgagtggcc catgtggacg caccagatcc
    17221 agatgccgcc ggccaagttc gtgcccgacc atgccgggca gcccggcgta aacgtgaaca
    17281 ccatcgtggc cagcggctgc gtggtaagcg gctccctggt gcgcaattcc gtgctctttc
    17341 caaacgtccg cgtcgagtcc ttcgcgcagg tgcaggacag cgtattgatg ccaggcacgg
    17401 tggtgcgctc gggctgccgc atccgcaagg ctgtggtgga ctgcgggtgc gacctgccgg
    17461 tagggctggt cattggtgaa gacgccgcgg aagacgcacg ccgcttccgg cgcaccgaag
    17521 ccggcgtggt gctcgtgact gccgagatgc tcagcgctct gggctgaaat acacagccga
    17581 gctacagcgc cgtgctcacg gccaggctgt tcacggtgct ggccagcgtg acgcgacgaa
    17641 tggccttctg gtcgtcccag gtcaccacca ccagcacctc ggtcaagctg tcgggcaggc
    17701 gcacgtcaaa gctcgcctgg ccaacaccgt ccgtcacggc cgtgtcgatg aggtccagcg
    17761 ccaccgcctc gtccagcggc gtgccatcgt ccgggttcac catggtcatg gtgaacaccc
    17821 gcaccgcggc attcgccgct gacgttccgg ctgcattggt gaccgtgacg gcaaaggcgc
    17881 cacttcggct ggtgtcccaa ctcatggtcg tgccgggcac gacctgatcg aaggtggtgg
    17941 gcgccgccgt gccgccgccg gacgtgcctc cggacccgct gtccgcaccg ccgccgccgc
    18001 cgccacagcc caccaaggcg gctgaaaccg ccaacaccgc gcaagagatt gctttgttca
    18061 tgtcggatac cttcggttgc cgctcaggga gcgcggtagg tcaaggacgt cacgggcgtg
    18121 gtgtaccaat ccttgtttga cgtgcccccg gtgcgggccc agtccacgat ggcgggatag
    18181 gcccggatga catcggtccg ttcttccggg tgcttccagc tggtcgggat gtccagcgcc
    18241 cagggccggc gctgcgcgtc catgtaggtg gtggtggtgc ccgcggtcgt gttgtccacg
    18301 ccggtcttga aaagcgatgt ggtggccagt gccgtgggcg gtttgcccgg caggtgaact
    18361 tcacggccac gctcacccac gcggaagatg aacgggttgt agggcggcga gaacaggctt
    18421 gaagcccgcg cgctgctgaa gttgacggtc agcttgtagt tcttgatggg caccgccggg
    18481 cacccggtct gggaattggc gaacgagcag cccgtgggcg acggcatggc cgccagcgac
    18541 gagggcatca ccacaaacac cgcctcggac tgcccggctt ccacagccag cggcgtggcc
    18601 gctgcaccgt tgaccgacag cgtggcactg ctcaccacac tggcgctgat gcccggaaag
    18661 tgcacggcaa agccgctgtc caccaggcca ccacgggcca cggcacccag ttccatctcc
    18721 accgcagcca cattgcgctg ggcgttgagg atttccttgg agcggtagcg catcaccacg
    18781 tcattgaggt cgtagtcgcc ccgtgcaggc cactggtctt cgtaggccag tgtgccccag
    18841 cccgtggcgc tgggatagga acgcgagaag gcgcgtgtgg cgtcgttcgg gtactcgtcc
    18901 aacaggtcct tgaccccgtc accgtcggtg tccgcgtctg cggcagagac caagggcgcc
    18961 agcgagtcca ggttggccag cgacgtgcgc gggttcacgt ggatgcccag cacgacgtcg
    19021 ttgaagtcat ggtcgccacc ctgctcgcgg ttgatgtcct caaagccgat gaccagcaat
    19081 tggtagtcgg gtcgcgaggt gctcagctca cgcaagatca ccgtgtggac attcaggttc
    19141 tgtgtgctgg tggcttcggg gttcaaggcg cgcaacgtgt agaaaacgtt cttgctgctg
    19201 acgttgtcct tcacaccgcc ggtcctggtg ccattgaggg ttcggcccgt gggcgagtag
    19261 ccgtcggaca cgatgaagaa gcccacgccc gtgccggcag ggaacgagcc caggtacacc
    19321 gtgttctggc gcgtgctgct ggcctggtcc agcggcgaag gcatagacgc gttgacaaag
    19381 acgatcttct cctgcacctg atcgcgggaa gtcggcgggt tggccaggtc gtaagtgaag
    19441 taacccacgc tgttgcggta gcccgcgcct tcgctaagga aggtgaccca gacgtccgcg
    19501 gttgcggaca actgcagatt ggtcttgctg tcactgtcgg gtatcaggcc ctgggagttc
    19561 aggcggatgt cgcgcccttc aggcagcgaa aagctgatct tcgaggccag gttcgtgggc
    19621 aatgcaggga gccctgcggg cagggtgcgc gtgaattcgg cgtacagcgg tgacccagtg
    19681 gaggccacgt agggcagtcc ggtggacacg gtgccgaagt actgccaatt gatgagcccg
    19741 ccggtgacgg tgtacccaca caagagggta cttaagagtg caaaaaatgc gatcagcttg
    19801 ctgcgacggc tcatgcccat gcctccgtag gtggtgacct cttttcggtt cgtcaccgga
    19861 aagctttaat cggcgagtgt cattgtcagc acataatgca tttttgccgt ttatggcctc
    19921 tatccagcgg aagggctcac gtaccggtca cggcccgcct gcttggcctg gtacaggcgc
    19981 cggtccgcca ggtccaacag gtccaacgcc tctggcacgg cgtccacttc tgagctggcc
    20041 aggcccaggc tcaccgtcac ttcactgagg tgctggcctt gctcatccgg gatgcgcaga
    20101 tcgcgcacga cttgcaaggc gcgttgggcc acggactccg cgcccttcag gtctgtatcg
    20161 ggcagcaggt agacgaactc ttcacccccg taacgggcca gcaggtcgcc gccacgcagc
    20221 agcaccgact tcaacgccag ggccaccgca cgcaggcagc ggtcgcccgt ggcgtgaccg
    20281 aagcggtcgt tgtactgctt gaagtggtcc acatcgatga gcagcgccgc catcggctgg
    20341 ccggcgcgtt ggctcacccg ccagtattcg ttcaggcgtt catcggcctg gcggcggttg
    20401 gccacgcccg tcaacgcgtc cacatagacc agccggcgca gcatgtcggc ctgctgcttc
    20461 agcatcacct gggtgcgcac gcgtgcgcgc acgatgcgag gctccacggg cttgctgatg
    20521 aagtccgcgg cacccacgtc caggcccttg acttctgcgg ccgagtctcg gtgcgccgtg
    20581 atgaacacga ccgggacatc gcgcaagatg ggagcggact tcagccgcag gcagacctca
    20641 tacccgtcca tgcccggcat gtcgacatcc aacaggatga ggtcgggtgg ctctccctgt
    20701 agcagctgca aggcttcggc gccgctgcga gccatgatca gttggtggtc cagcgcgagg
    20761 gcgctgcgca gcgccaggat gctgttgatg tcatcgtcca ccaacagcag gcgcggccgc
    20821 cggaccaggt cttcctgcgg gtccttggct gaagccgggc tggggttcag gctcatattc
    20881 gtggtgtaca tgtcccggat ctccccaggt gtgcttgcag cccgctgagt gcttccaggg
    20941 ccgcgggaaa attcaactgc cgaatggatt tgtccagcac actgccccaa ggctggactt
    21001 cctcgcgggc cgacaggcca agatgcgagc cgacgtccag ggcacccagg tcgctgcgtt
    21061 ccagcagggc ccggagttcc tgcaggttgg cggcttgcaa ggggtccagc ggctcttcac
    21121 ggccttctcg cacccacgtg ccctcagacc accgggactg caaggcttcc agccggggaa
    21181 gactgctcag catcacggcc acggcgcgct cggccaacac catggcggca gggccagacg
    21241 gctccgcagc caactcgcgt tcggccgccg ctgaagcctc atacagctcc tgcacgccca
    21301 atgtgccggc gctccccttg agcttgtgga acaggcgcga agcctgcaga gaattggcac
    21361 ggctgacgtc ctgcaacact tggtctggca gtttgtgcag gtcctggatc aagcggtcca
    21421 gcatctccag gtacagctcc tcgtcgtggc ccaggcgctt gagcgcgcca gccaggtcca
    21481 cgccatgggc ctccgccagc gccatgggca gcaccgagcc ctcgggcggg ggggctggca
    21541 gtgacgcctg gtgccgcgtc aggtccagga tgaccatgac caggtgggcc aggtcaaagg
    21601 gcttgcccac gtgctcattc atgcccgcat ccaggcacga ctgcctgtcc gccgctgagg
    21661 cattggcggt gacggccaca atgggcagcg actgcagggc gggcattgcg cgcaggcgcc
    21721 gtgtggcctc caggccaccc atcatgggca tctgcatgtc catcaacacc acgtccggca
    21781 aggacgccgc atgagcaagc cgctcgacgg cctcctggcc atcacaggcc agcgaaacgt
    21841 gtgcccccgc agcgcgcagc aattcggtgg cgacaaactg gttgtcgggg ttgtcttcca
    21901 ccagcaagat gcgcaagccc atcagcacct tgtgcatcgg gctggcgcgc ggcgcggcag
    21961 caggcaactg cccagctcgt tcgctctcgg catgcgtcat cgcagccaac aggcgctgtc
    22021 gggtcaaggg cttggacagc gcgtgaagac cattgctctc tcgaacttcg cgcacagggc
    22081 tggtcatggc ggccaggcgt ggcttggtca acacgagcca gacgggtgca ggccccggcc
    22141 cgaagtgcgc cgcaacggcc gtgcgggctt cagtcaccca ctgttcggca cacaggatgt
    22201 aggtcggcgg cgcaacagtg ggcattcggt cctgtgcgga ccaatgggtc aggtcgcttg
    22261 cacattccaa ctgccagtgc tgctcgtggg cgatggctgc cacggcactg ccaacggtag
    22321 tacttgggtc cagcagcagc acggacgcgc gcgccggccc ctgcaccttg ctccgcgacg
    22381 caccttgcac cggaaccaca gcctcgaagc ggctgccctc gcctagctgg ctgctgacac
    22441 taaggattcg ctgaaaaagg tccacgccga tcaggctcga agcgttgagg ttcgatgaag
    22501 caacagaccc ttgcgatggc ggctgatcag agcgcgagct tcgagcggta ccgcaagccg
    22561 acgcggcgcg acgagttcct ggcgacgatg aatgcgatcg tgccgtgggc gcagttgtgc
    22621 gatgtggtgc agccgtacta ccccaagggc gttggaggac ggccgccgat cggactggag
    22681 cgcatgctgc gcatccactt catccagcac tggttcaacc tggccgacga agcctgcgag
    22741 gaggcgctgt acgacagcgc gagcctgcgc cgcttcgtgg gcatcgatct gggtcgtgag
    22801 ccggtgcctg acgcgacgac gatgctgaag ttccgtcgcc tgctcaacga caacaagctc
    22861 ggcgagcaac tgtttgccaa ggtcggcgag gtgctgcagc aaggcgggtt caagctcaag
    22921 accgggacga tcgtggacgc cacgatcatt ggcgcgccga gttcgacgaa gaacgctgac
    22981 aacgcgcgcg atcccgagat gcaccagacc cgcaagggca accagtggta cttcgggatg
    23041 aagatgcaca tcggcgtgga cagtcaaagc ggcctggccc acagcgccgt ggtgacggcg
    23101 gccaacgtgc acgacaaaca tcccctgccc gaactgctgc atggcaacga gcgccgggtg
    23161 tatggcgaca gcgcatacgc cagtcagaag gacctgattg ccggcaaggc gccacgggcc
    23221 aaggacttca ccaacgagcg ggtgcgcaac cggcaaggcg aagttgacga ggctgcgcgg
    23281 gcgaagaacc gcaacaagtc gcgcatccga gctcgggtcg agcatgtgtt ctgcgtggtc
    23341 aagcgcctgt ggggcttcag caaggtgcgc taccgcggcc tggccaagaa cgccacgcgg
    23401 gcgtttacgg cgcttgcgct ggccaacatc tacttggctc gagggcgatt gatggcacag
    23461 gtgcgcccat gagggcaaag cgcgggccca gaggcccgca aacaaggcct tgcgggccga
    23521 ccacgagccg aaactcaaca ccacatcgcg catcatgaaa cgtaccgcac acgcatctct
    23581 cgatgcggcc acttcttcag cccatcccta atggagccgc ccaacgcctg gacaatgccc
    23641 tgcgccagcg acaggcccaa cccggcgccg ccctgccgcc gcaccgcgcc tatggcgccc
    23701 tgcgcgaagg gctggaaaat ggactccagc tcgctggtgg ggatgcccgg ccccgtgtcc
    23761 tcgacgacca ggtgcaacgc aacttcgtcc tgcatatccg ccgcaccgcc cgtcaccgac
    23821 agactcagtt tcacctcgcc ttgctgggtg aacttgacgg cgttgcccag caggcagttc
    23881 agtgcctgcc gcagccggcg tgcgtctgac accaccactt ccggcatacc gggttcggtg
    23941 cagacaacca actccagtga cttctcggcc gcctgcaaac caaagacctc acccaggtcg
    24001 gtcagcatgg cctccaggtc catcggcgcc aactgcacat tgatc
//
LOCUS       DAOORV010000047        23939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2576_length_23939_cov_7.176227, whole genome shotgun sequence.
ACCESSION   DAOORV010000047 DAOORV010000000
VERSION     DAOORV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_23939_cov_7.176227"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="PKV56_08270"
     CDS             complement(<1..1407)
                     /locus_tag="PKV56_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOX67851.1"
                     /translation="MLNSNWRSPMNPSQSPWQEGSRSSRKWLLLAACAYSVFAVYGSL
                     VPLVFKPIPFDQALQRFQDIPYLQLGIASRADWVANILLFIPLSFLWSGVAWPTQGVF
                     RRLVTGGIVLSMACALSVSIEFVQQFFPQRTVSLNDIIAETVGATIGVVSWTLFGPAT
                     LRWFHALALVKGTANVAARLLLAYLLVLVGYNVMPLDLTISPVEIYHKWREGKVILIP
                     FSRPYADSAQHAYDLLTDIAIWAPAAVLWQLAYQKSRLHTWKMVVAAAATIELLQLCV
                     YSRVSDTSDVITAALGAAIGLTAERSMRPADPLRPVEANAGVLPWVLASAAWMAVLAA
                     VFWYPFDLNLDSTFLRERLAGLQKVPFEAYYFGTEFRAVTEVLRKSLFLAPLGFLYYR
                     LGRQLPVSWPRGLVHGSLLVLIAAVVTSIEVVQIALPGKNADLTDGVLEFLGGALGYA
                     GSRFVDERLTPRHRSDARD"
     gene            1837..3717
                     /locus_tag="PKV56_08275"
     CDS             1837..3717
                     /locus_tag="PKV56_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOX67852.1"
                     /translation="MLACQHPVAPVALPLAFVLWSVLIAWKPGLWLFALTAALPLSNF
                     SPWTGWIAFEEFDLLLWGVVAGGYARLGLARWTAGAAPPGARTDLVCLGLAGVLGAFG
                     IAALVRGMLVASSGASGLFESYADPLNSWRVFKPLLHAALIWPLLRWEISRNAESATR
                     CLASGILVGLTLVTLAVVWERLAYTGLWDFSARYRTTALFWEMHVGGAAIDVYLVLAT
                     PFVAWALWTTRSPLLWALAAVLALLTGYACLTTFSRGVYGAVAGSLLLLALLLRGRRL
                     PRIRWRVVGGFALAVALTLEVAAVLGLGSFMRERMAASDHDMDGRLKHWENGLALLQS
                     PSDWMLGIGLGRLPAAYAQAHPGSQFPGEVAFIPPAGGQPTGTVRMAGPKSPPRFVGQ
                     MALTQRVALRPVPGHVVGFDVRAQAEAVLYFQLCEVHLLFPRNCQSGVMRVSPGSAAW
                     RHAAVRLNGPLLDVGSFWAPRMGVFSVSVLNHASAAELDNLSLVGPDGVELLANRDFS
                     EGLARWFPAAQGYYFVPWHIDNLFLELLIERGVVALLAFALFMGWTLWRLVGAAGRGV
                     PIAPFLIASLCGGLCVGLVSSVLDVPRVAFLMFLIALFSAEVVRDPLRSALPGSGDER
                     PA"
     gene            complement(3718..4773)
                     /locus_tag="PKV56_08280"
     CDS             complement(3718..4773)
                     /locus_tag="PKV56_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOX67853.1"
                     /translation="MAYEALRSALRATPKTWLVTGVAGFIGSNLLETLLELDQHVVGL
                     DNFATGHQRNLDEVASLVGAQRWARFRFIRGDIRSLQDCQSACQDVDFVLHQAALGSV
                     PRSLADPVTTNAVNVDGFLNMLVAARDAKVRRFVYAASSSTYGDHPALPKIEDRIGAP
                     LSPYAVTKLVNELYATVFTRAYGLDSIGLRYFNVFGPRQDPDGAYAAVIPKWISALID
                     GRPVHIYGDGETSRDFCYVANAVQANLLAACATGDTAVNQIYNVAIGDRTSLNQLFEL
                     LRKNLVASYPQLRDVQPHYADFRAGDVQHSQADITKAVERLGYRPTHRVGEGLAQAMQ
                     WYMMQAKRNDSVLRPAH"
     gene            complement(4773..6065)
                     /locus_tag="PKV56_08285"
     CDS             complement(4773..6065)
                     /locus_tag="PKV56_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOX67854.1"
                     /translation="MNARTPVVAVVGLGYVGLPLAVEFGKRFTTIGVDLSERKVAAYR
                     RFEDPTGEVSTEQLRAATLLTCTTDGAALAEADFIIVAVPTPIDDARRPDFQPLVGSS
                     ETVGRHMKRGAIVVYESTVYPGATEEVCVPVLEKYSGFKWKQDFNIGYSPERINPGDK
                     TRTLTKIVKLVSGDTPETLAQVKDLYAGIIEAGVYPASSIRVAEAAKVIENTQRDLNI
                     ALMNELSLIFNRMGIDTIEVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAEMLG
                     YQPQVILAGRRINDSMGKYVAEQTVKRLIASGQAVKDADIVVLGMTFKEDCPDLRNSK
                     VIDVIHELQSYGVKVHVHDPIADSDECLHEYGIPLTAWEDLPRAAAVIAAVAHQPYKD
                     MGLATLLSKLQPGGVFSDVKSAHDPVAVQEAGYALWRL"
     gene            complement(6195..7307)
                     /locus_tag="PKV56_08290"
     CDS             complement(6195..7307)
                     /locus_tag="PKV56_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67855.1"
                     /translation="MTTAQASDIDPVWQAHAGEARLLVSLLRTSRLALLYCDEGTEKS
                     SFLTSALMPLLGRRAEDRFMPADARETGVAAPPADRRKRPAVHNAACTREFVVQFDDW
                     SDDPLPALLARIHQVANTCEAERSAPWQRLGDTLEALGTRLDASFIILLDRFEAFLCA
                     PPDHAGNARFASDWVDAVLRAKVPVSFLVAVDEDARPRLGHLRNRIAGFDDFSLKLTR
                     PAPTLRPPKATQPPDAVRHVAQPAPPVLTEAVMVSPPALADPPKAERAEVTQPKRKIR
                     PPKVKRPVQPRSEVKAEDVYALIESTLSRTVTEVSDDPFSAGQAATEPSARAPEEVPP
                     TNSAVASSVLPRSRWQSVITRIGRMLRGANRPGRDA"
     gene            complement(7356..8633)
                     /locus_tag="PKV56_08295"
     CDS             complement(7356..8633)
                     /locus_tag="PKV56_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67856.1"
                     /translation="MRLDTLLLQLALLVSTVAQAASPPDAAPDDLDALSLADKAPTTA
                     PQAAKAWRIFLEGAGVRTGVGGSDSNFETTRGSIDVRFDSSIAPGLRAVFSDRLDLLH
                     KNGSARAHDVNTLREAYLSWARTDEQILDVGRVNVRHGAAFAYNPTDWFKEGALRSIV
                     SPDPAVLRENRQGTVVLQGQQLWSAGSITATYSPKLGREPNPGTFSLNVGATNPRNRW
                     LLAGSTKFSDKLNTEVLLYGGDNTRMQVGLNVSALISDSAVVFGEFTTGKGRSLVAQA
                     LELDQARRHQERAAVGVTYTTPLNLSLTAEAQYNSAAPDRSQWKALPGLDPSARLRVL
                     GTAPTLQDLPVRNALFFYANWKDLLVRRFDLSAFVRRDSETHSRHQWIEARYHWDSAE
                     LALQWQLYSGNAASVYGSVPERRAIELALRFFL"
     gene            complement(8636..9418)
                     /locus_tag="PKV56_08300"
     CDS             complement(8636..9418)
                     /locus_tag="PKV56_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX67857.1"
                     /translation="MNTTLALIVSLAWVGLGLSSPALAAPDAQAILAASDAVRNPSKS
                     FAVTVTLLEYRSAKQTDNNTLTVYSKADASSGQYRSLLRFVAPQRDANKLMLKNGNDL
                     WFFDPASQASIRLSPQQRLLGQASNGDVVTVNLAKDYAATLESEEDLTDGERVMRRCY
                     KLALAAAAPDVTYHRVEMWIETTSSRPVKARFFAESGHLLKTAYYRKFQPHLGRERPT
                     EVVIIDGLDPAWVTVMRYSDYAWREVPESWLQRDYLPRFKPE"
     gene            complement(9444..10844)
                     /locus_tag="PKV56_08305"
     CDS             complement(9444..10844)
                     /locus_tag="PKV56_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX67858.1"
                     /translation="MNTLSLAWRNLLRNRRRSLMTLIAMILGLTAVLLFGGYIRDINY
                     SLQTNFVTSTGHLQIQHKDYFRFGSGNPSAYGIQNYERVIAAVRKDPVIAPMLAVITP
                     TLQFGAIAGNFAASVSRTVFINGTVVEDQNQMREWNDYGQRDLSSRHLSLSGTPPDTA
                     VIGTGVARVLHLCAPLKVEDCAPEDSKKAVDGAALPDDISDLAMLGTTKNAVTKPDAG
                     PRIEILAANARGAPNVAGVNVLAAEFQGVKEVDDIYIALHLSQAQKLVFGAAPPQATA
                     IAIQLKHTAQIPEVRARLETLLTTELRGEPLAVLDYETLNPFYGQTLAMFAAIFGFIS
                     MLMGSIVLFTVSNTMSMAVVERTSEIGTLRAMGLRQSGIRAMFVSEGIVLGCFGAVLG
                     IGIAAALAWVINSLGLTWVPPGRNEPVPLAVNLAGAHWMIIASAVGLVVVAALSAILP
                     AARAARMTIVDALRHV"
     gene            complement(10841..11575)
                     /locus_tag="PKV56_08310"
     CDS             complement(10841..11575)
                     /locus_tag="PKV56_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67859.1"
                     /translation="MPGERVRNLAHPVVLKGVSRTYRLDSVDVPALTDINLVILPNRF
                     TVLSGPSGSGKTTLLNLIGCIDRPDKGTIMVGGRPVQTMEDDALSDFRAHQLGFIFQN
                     FNLLPVLTAFENVEYPLVLTGMPADKRRARVAKLLEAVGLADRAGNRPGQLSGGQRQR
                     VAIARALALRPQLVLADEPTANLDSKTGVEIIELMRSMQKKHRVSFIFSSHDPKVLEA
                     ADDAVHIHDGRITSIERRLAMAESRT"
     gene            complement(11890..12339)
                     /locus_tag="PKV56_08315"
     CDS             complement(11890..12339)
                     /locus_tag="PKV56_08315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX67860.1"
                     /translation="MAGAASHSEAGDGAQLLLTLANDREGFDQTRLAVLRHLAPHGAS
                     QQLLFKVELILEETLMNVIWHAFPDDEVHPITMSVQVEPEDIVMHFEDDGMAFDPLLA
                     PAPKLPTSIEKAVPGGLGLMLVRKYARSVAYERNAGRNCLTVRVART"
     gene            complement(12381..12716)
                     /locus_tag="PKV56_08320"
     CDS             complement(12381..12716)
                     /locus_tag="PKV56_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX67861.1"
                     /translation="MDVTELDGGVTCIRLSGRLDSPGIDRIEVKFTAAVVSRAHNAVI
                     DLSGVDFLASMGIRMFIASARGLHLKGAKMVLFGAQPLVHSVLEHVALDQIIPIAANE
                     TQALERLQG"
     gene            complement(12815..13972)
                     /locus_tag="PKV56_08325"
     CDS             complement(12815..13972)
                     /locus_tag="PKV56_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX67862.1"
                     /translation="MNKSLPSENDSNQDLTILTITRSTIAPKVAAEKREHFLVAIQGS
                     QTGRRLRFAADHPITVGRKMVCDFVLPDAEVSGKHCQISAAPDEADATVTDLQSTNGT
                     FVEGKRLIGSIRLPNGGLLQLGGHVLKHEYRLPSELEQSLELDRDLEKASSYVKALLP
                     APIREGPVRTDWFFLPSTTLGGDAFGYQQLDEDTFAGYVVDVSGHGAGAAMHSVSVMN
                     VLRQRALPNTDFRDPAQVLHSLNTTFQMESHGGMYFSIWYGVYEPKTRLLRYSSAGHH
                     PSYLAQPSIQELIPLQTRNLMIGAMPEAKFISSSVEVMPGSVLYVFSDGVFEVVTKEG
                     TQWGLQDFLPLLRRPESAEVAEPERLYRAVQDVARPGALDDDFSMLVVTFV"
     gene            14162..15985
                     /locus_tag="PKV56_08330"
     CDS             14162..15985
                     /locus_tag="PKV56_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOX67863.1"
                     /translation="MSAPSAGDGGAATQPVSRDEDDAPTRIATTQPVVMPSAGAREAD
                     SSATLPGTSLADGGVLQQVGRYRIHERLGRGGMATVFKAHDPGIGRDVAIKFLHASLC
                     EEPEYRARFLSEARAAGGLSHPNIVTVHDVGEIDGRPYMAMELLDGETLSDLLANNPP
                     LRVRDVVVMGIQLARALDYAHSRGIVHRDIKPGNIMQVKGTQTVKVTDFGIAHVESSG
                     SEQRTHLGDILGTPQYMSPEQAKGERLDGRSDLFSAGIVLYQMLTGQRPFQGDSLVSL
                     AMQIATAEPKPIEQLRPEVPAALRRVVSRSLAKLPDRRWASGKELAEALTRVLAEIDA
                     AELEKSRQRIVPLRVKWAATMALIVAVVMALAATVITQRQYAAMFSQATDYGASLARF
                     IAAQNAVSALSEDWVAVEVSIQEVMKARDFQSIAVIDREGMVRATSAGSTGLAYRAPA
                     SEALGERKGGVTVTRFTADGESVLGFEAPITFQDKRVGSVSLGLPEKPLIAVARLSIG
                     LMVALVVITVLAVAVAMFFVANWFAQPIKLLGESMQEIGKGRFDHRIAEQRKDEFGQL
                     YATFDQMAQALADRQSGAVVGPPTPTPLPTRAGPGPASNKE"
     gene            15993..17030
                     /locus_tag="PKV56_08335"
     CDS             15993..17030
                     /locus_tag="PKV56_08335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOX67864.1"
                     /translation="MSARQVLLVAAAWVVVWLAGCASAPPPSVTAPATEVRSGNGNGD
                     VLGRSDRFVIYQPDNDDTLRSIAGQFLGSPDRDWVIGDFNRLSRPVAGQPLVIPIAPI
                     NQVGVQGDRYQTVPILCYHRFGGNSSKMSVSSENFAAQLDWLARNDYRVIPLSQLLGF
                     LQGREALPKRSVVITMDDGYESVHRHALPQLRKHGFPATLFVYTDFIGAGDGLSWNQL
                     AELQASGLVDIQAHSKSHRNLIERTTGETDERYRQSIDAEVRVPRELIERRLPQHQVR
                     HFAFPYGDANESVLDALLRQQYQLAVTVYPGGNAFFATPYMLRRTMIFGDIDIEAFKA
                     KLQISRATPAP"
     gene            17027..17863
                     /locus_tag="PKV56_08340"
     CDS             17027..17863
                     /locus_tag="PKV56_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67865.1"
                     /translation="MRSLPALLLGGCVATLLGCSSTAPPPAARSVASPQPTAAPSRSG
                     FEREYRERAANAARQGRLAEAALAWEVLTVLRPDVTEYREALADTRRQIDAAVADRLP
                     RAAQAQRRGDLDAASQLYLSVLALQPEHATAADALRAVERERVKRQQLGRSSRTMLTR
                     RAATDAEVMADKAAVAGGADRNELEHVAMLARQGELDSAIGLLERRVAGSRNDRAARE
                     LLADLYERKAEEAVTARDSAAAIVLLEKSVRLDPSDTRAAERLKQLRASAATRPADSK
                     SR"
     gene            complement(17897..18814)
                     /locus_tag="PKV56_08345"
     CDS             complement(17897..18814)
                     /locus_tag="PKV56_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOX67866.1"
                     /translation="MLAIRLILLCSTAVLVTSCGTPPAKAPVPVPAVTEPAPVETPPA
                     PPPPPPLTLQQAQKMALASADLLEAGNEEQAKADLQRVLASDPGNKLAQNLMRQISAD
                     PVAVLGRESFSYTVRSSDTLSRIAGRFLGDIYSFYILARYNDIKIPRQVAGGQVIRIP
                     GKAPPPGVLENEARRDKAVAPTNPTPPVTTVAPAAPVTNEPSAGEKALTSAEAAERRG
                     DLDRAYSDYRTATGGELSNDALAKAEQVRRRLVTRFTLAARGAFAKQDLDGSIAQWDR
                     VLQLEPGNDTARLEQQKARALKEKIKSLK"
     gene            18861..20282
                     /locus_tag="PKV56_08350"
     CDS             18861..20282
                     /locus_tag="PKV56_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOX67867.1"
                     /translation="MEAADYRRCAESPASTSGSSPAAARCSAGQESLFRALWRLALGA
                     SCLLSCVSAHAQAAAGFDSCVRSLRAQLPKQPPVSAETFDRYTRGAEDLRPLIDRSTA
                     TQPEFKLPVWDYLARLVDAQREADGRAVLDREAVALAAIERRHAVDAATAVAVFGVET
                     DYGRVPGRYPVVDATLSRACLNLASRERKAHFYAALWLLQEGLVRPETFRGSWAGAFG
                     LTQFMPGTFLQNMDDGDDSGSIDIVDSVPDALATTARYFKGLGWTAGLPWGIEVSVPR
                     EQALAWNALERDHACLEAEMPAGLCRGLDQWAAAGFRRIDGGALLPEGPGSESPLRPD
                     TPAALLMPGGIDGPAWLVTRNYQAAWRYNRADAYALAIGLLADRLRGDPPMRAAWPTD
                     DAALSRAEFAELQMLLRQRGHCEVTADGHDGPRTRSAIEAEERWLGNNPTGRAGARLL
                     AALRAATPMEPVESPACADAGQR"
     gene            complement(20313..20792)
                     /locus_tag="PKV56_08355"
     CDS             complement(20313..20792)
                     /locus_tag="PKV56_08355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX67868.1"
                     /translation="MTDMLIKLYDLPPGAAGESSAGSPAALVRKPIGPEHEAVVAWIQ
                     QAFGSRWASEARVALQNRPVSLYIAMQGARLIGFACYDATARGYVGPIGVDEAARRSG
                     TGAALLRAALADMRAAGYGYAIVGAVGAKEFFTRVAGASDIADSTPGLYAGMYRGGD"
     gene            20905..21882
                     /gene="glsA"
                     /locus_tag="PKV56_08360"
     CDS             20905..21882
                     /gene="glsA"
                     /locus_tag="PKV56_08360"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOX67869.1"
                     /translation="MPQNQTDGSMISDLLQSIHARYSSLHDGNVATYIPALAQANPDQ
                     FGVCVVSTRGDIFEVGDSRTEFTIQSCSKALVYAHALSTHGRTKVLERVGIEPSGDAF
                     NSIALDASNRAFNPMVNAGAITTAGFIEGADFAERRAALLRCFSAAAGRELEVDETVY
                     ASERETGHRNRALAWLVTNFDRMDPATNMETLDLYFLQCSVRVNARDLAVMGATLANL
                     GVNPLTKETVFDMASMQDVLSVMATCGMYDYAGEWVFRVGIPAKSGVGGGILGVVNRQ
                     LGIGTYSPRLDARGNSVRGIRAFCDLSDDLGLHAFNFMNSGSTFLKHLV"
     gene            complement(21920..22768)
                     /locus_tag="PKV56_08365"
     CDS             complement(21920..22768)
                     /locus_tag="PKV56_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HOX67870.1"
                     /translation="MKAPPRLQPLVDEGLIDSVVRQLMSGKEAMVFVVRCGDETRCAK
                     VYKEATQRSFRQAVDYTENRKVKNSRQARAMAKGTRYGRESQEAAWQSAEVDALYRLA
                     AAGVRVPKPYNFHDGVLLMELVTDAHGDAAPRLNDVAFTPDEARTHHASLLTEVVRML
                     CAGVVHGDLSEFNILLAADGPVIIDLPQAIDAAGNNHAPRMLLRDVANLRSYFGRYAP
                     ELLATEYGAEIWDLYVRGMLTPEVVLTGRYEHQTGAVDLSGVMREIDDARAEEAARRL
                     RMADSN"
     gene            22962..23345
                     /locus_tag="PKV56_08370"
     CDS             22962..23345
                     /locus_tag="PKV56_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX67871.1"
                     /translation="MKAHLLSIAALLVASSAAWADPANDKAMLELATHSGCMTCHQIE
                     SGAKGPDGLAPVGPPWSDVAAKYKGTKGAEKQLTHIVMTGSNPYASHWKGKVSGLAMP
                     PNEVAIKEADARRLVHWILKLDSKG"
     gene            complement(23368..>23939)
                     /locus_tag="PKV56_08375"
     CDS             complement(23368..>23939)
                     /locus_tag="PKV56_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67872.1"
                     /translation="YGWSDLGAVPWADEAARAAWTEALLERLVALNAKRAAEEAGTLP
                     GSPPGGTVRWLRPEFQDPARRMAGASVAPQPEQTGLDGVESQAEAAARADLAETEVVE
                     APAEGDAGGAAAPQAAAATTQPWPPTLPEQVRAVAQLLSASPAPLPLATIEASFKGKG
                     PWKKGLPRILDTLEAVGRARRDGAGWRGS"
BASE COUNT         4014 a         7583 c         7929 g         4413 t
ORIGIN      
        1 gtctcgcgcg tcggatctgt gtcgcggtgt caaccgttca tccacgaacc gactgccggc
       61 atagcccaga gcgccaccga gaaactcgag cacgccgtcg gtgaggtccg cgttctttcc
      121 gggtagcgcg atctgcacga cctctatcga ggtgacgact gctgcgatca ggaccagcag
      181 gctgccatga acaagccctc ggggccagga cacgggcagt tgccggccca ggcggtaata
      241 gaggaagccc agcggtgcca gaaacaggga ctttcgaagg acttcggtga ccgcacggaa
      301 ctccgtgccg aaatagtagg cttcgaacgg gaccttctgc agtccggcca gcctctcgcg
      361 caagaaggtg ctgtccagat tcaggtcgaa cggataccag aaaacggcgg caagaacggc
      421 catccaggcg gcagatgcca gcacccatgg aagcacgcct gcgttcgcct cgactggccg
      481 aagcggatct gccggtctca tcgatctttc agcagtcaga ccgatggctg ccccgagtgc
      541 ggcagtgatc acgtcggagg tgtcgctgac acggctgtag acgcacagct gcagcaactc
      601 gatcgtggct gccgcagcca ccaccatctt ccatgtgtgc aggcgcgact tctggtacgc
      661 caactgccag agcacggctg ctggcgccca gatggcgatg tcggtgagga ggtcgtaggc
      721 gtgctgcgca ctgtcagcgt agggcctgga gaagggaatc aggatgacct tgccttctct
      781 ccacttgtga tagatctcca cggggctgat cgtgaggtcc agcggcatca cgttgtagcc
      841 gaccagcacc aggaggtagg ccagcagaag gcgcgcagcc acgttggcag ttcccttgac
      901 cagtgccagc gcgtgaaacc aacgcagcgt ggcaggaccg aaaagcgtcc acgagaccac
      961 cccgatcgtg gcgccaaccg tttcggcaat gatgtcgttg agggagaccg ttcgctgagg
     1021 gaagaattgc tggacgaact cgatcgacac gctgagtgcg catgccatcg acaagacgat
     1081 gccgcccgtg acgagacgtc gaaatactcc ttgggtgggc caagccacac cggaccacag
     1141 gaagctcagc ggtatgaaca gcagtatgtt tgcgacccag tcggcacgag aggcgatacc
     1201 gagttgcagg tacgggatat cctgaaatcg ctgcagcgcc tgatcgaatg gaatgggctt
     1261 gaacaccagc ggtaccaggc tgccatacac ggcgaacacc gaataggcgc aagcggccag
     1321 aagcagccat ttgcggcttg agcggctgcc ttcttgccac ggactctggc tggggttcat
     1381 tggcgagcgc cagttgctat tgagcatgaa tgcgtggcgc gcgatgtacc gccggtgcaa
     1441 cggcgccgtg tcatgtccgt gagcgagttc atcacgcgga attgtggctg ctgcagcgtg
     1501 ctcgcccaag ggcttgtgca gtgacatcgc acgggtgcga agccgccggg ctcatcgctg
     1561 ccgcgcgtcg attggaaggc gcctcgctat cctcagcgcc ttgcccggtg tcgggacccg
     1621 ttcgcgcaga tcgcaacagg gatgccttgt cgacccgatg cctggcgctt ggagaactga
     1681 tttcttgatg aattttcaac gggcttgtag cctttccggt ggttgctgcg ccccgttgcg
     1741 agacgtggcc tgatgcgtca ctccgggcgg ccgcagttcg aatcagccgt tggtgtgtcg
     1801 gcgcttgcgg ccctggtcgc attgatcgca ggcttcatgc ttgcctgcca gcacccggtg
     1861 gcgccggttg ccctgccgtt ggcgttcgtg ctctggtcag tcctcattgc atggaagccc
     1921 ggcctctggt tgttcgcgct gaccgctgcg ctgccgctgt cgaatttctc cccatggacg
     1981 ggctggatcg cgttcgaaga gtttgatctg ctgctctggg gcgtggttgc aggcggatat
     2041 gcccggctcg ggcttgctcg ctggaccgca ggcgcagccc cacccggcgc gcgaacggat
     2101 ctggtttgcc tcggcttggc tggcgtgctg ggagcgttcg ggatcgctgc tctcgttcgt
     2161 ggcatgttgg tggccagttc cggcgcttca ggcctgttcg agagttatgc cgacccgctg
     2221 aacagctggc gtgtcttcaa gccgctgttg catgccgcgt tgatctggcc cttgctgcgc
     2281 tgggaaatca gccgaaacgc cgagtccgcc acacgttgtc ttgcatcggg aattctggtc
     2341 ggcctcacgt tggtgacact ggcagtcgtt tgggagcgtc tggcgtacac gggcctgtgg
     2401 gatttctcgg cccgctatcg cacgacggca ctgttctggg aaatgcatgt gggtggcgct
     2461 gccatcgatg tgtacctggt gctcgcgacg ccctttgtgg cctgggcgct ctggacgacg
     2521 cgctcgcccc tgctgtgggc gctcgctgcc gtgctcgcgc tgctgacggg atacgcctgc
     2581 ctgacgactt tctcgcgtgg cgtctatggc gcggtggcgg gctcgttgtt gctgcttgcg
     2641 ttgctgctgc gggggcggcg tctgccgcgc atccgctggc gggtcgtggg cggctttgcc
     2701 cttgcggtcg cgctcaccct ggaggtggcg gccgtgctgg gtctgggatc gttcatgcgt
     2761 gagcgcatgg ctgccagcga ccacgacatg gatggccggc tgaagcactg ggaaaacggc
     2821 ctggccttgc tgcaaagccc gtccgactgg atgctgggaa ttggcctggg ccgcctgcct
     2881 gccgcctatg cccaggccca tccagggagc cagttccctg gcgaggtcgc gttcattccg
     2941 cccgccggtg ggcaaccgac ggggacggtg cggatggcgg gaccgaagtc gccgcctcgc
     3001 tttgtcggcc agatggccct cacgcagcgc gttgcgctgc gcccggtgcc tgggcatgtg
     3061 gtgggattcg atgtccgggc gcaggctgag gcggttctgt atttccagct ttgcgaggtg
     3121 catctgctgt ttccgcgaaa ttgccagtca ggtgtgatgc gcgtctcccc gggcagtgct
     3181 gcatggcgcc atgcagccgt tcggctgaac ggtccgctgc tcgatgtggg gtcgttctgg
     3241 gcgccacgca tgggtgtgtt ctcggtctcg gtgctcaatc acgcgagcgc tgcagaactg
     3301 gacaacctga gcctggtcgg gccggacggg gtggagcttc tggccaaccg cgatttctcc
     3361 gaaggactgg ctcgctggtt cccggcagcg cagggctact actttgtgcc gtggcacatc
     3421 gacaacctct tcctcgagct gctgatcgag cgcggagtcg tcgcgctgct ggcctttgcg
     3481 ctgttcatgg gatggacgct gtggcgtctc gtcggtgcgg cggggcgtgg cgtgcctatc
     3541 gcaccgttcc tgatcgcatc actgtgcggt ggcctgtgcg tgggcctggt gagcagtgtg
     3601 ctggacgtcc cgcgcgtcgc attcctgatg tttctgatcg cgcttttttc cgcagaggtg
     3661 gtgcgcgacc cacttcgatc cgccttgcct ggatcaggcg atgagcgacc ggcgtgatca
     3721 gtgcgcgggc cgcagcacag aatcgtttcg cttcgcctgc atcatgtacc actgcatcgc
     3781 ctgcgccagc ccttcaccca cgcggtgggt gggtcggtat cccagtcgtt cgaccgcctt
     3841 ggtgatgtct gcctgcgagt gctggacatc gccggcacgg aagtcggcat agtgcggctg
     3901 cacgtcccgg agctgcggat aactggccac caggttcttg cgcaacagtt cgaacagctg
     3961 gttcaggctg gtgcggtcgc cgatcgccac gttgtagatc tgattgaccg cggtgtctcc
     4021 agtggcacac gcggccagca gattggcctg cacggcgttg gcaacgtagc agaaatcacg
     4081 gctggtctcg ccgtcgccgt agatgtgaac cggacgacca tcgatcagtg cggaaatcca
     4141 tttcgggatg accgctgcgt aggcaccatc gggatcctgc cgtggcccga acacattgaa
     4201 gtagcgcagt ccgatgctgt ccagaccgta ggcgcgggtg aacacagtgg catacagctc
     4261 gttgaccagc ttggtgacgg catagggtga gagcggcgca ccgatgcggt cttcgatctt
     4321 cggcaaggcc ggatgatcgc cataggtcga actgcttgcc gcatagacga agcgccggac
     4381 tttggcatca cgcgcagcaa ccagcatgtt caggaagcca tcgacgttga ctgcattcgt
     4441 cgtgaccgga tcggccagtg atcgaggcac gctgccgaga gccgcctggt gcaggacaaa
     4501 gtccacgtcc tggcaggcgc tctggcaatc ctgcaggctg cgaatgtcgc cccggatgaa
     4561 gcggaagcgc gcccagcgct gtgcgccgac cagcgaggcg acctcatcga ggttgcgctg
     4621 atgcccggtc gcgaagttgt cgagtccgac cacgtgctgg tcgagttcga gcagcgtttc
     4681 cagcaggttc gatccgatga agcccgcgac accggtcacc agccaggtct tcggtgtggc
     4741 ccgcagtgcg gaccggagcg cctcgtatgc catcagagcc gccacaaggc gtagccggcc
     4801 tcctgcaccg ccacgggatc gtgcgcggac ttgacatcgg agaacactcc gcccggctgc
     4861 agtttgctca gcagggtggc caaccccatg tccttgtagg gttggtgcgc gaccgcggcg
     4921 atgaccgcag cggcccgggg caggtcttcc caagccgtga gggggatgcc gtactcgtgc
     4981 aggcattcgt cgctgtcggc gatagggtca tgcacatgca ccttgacgcc gtaggactga
     5041 agctcgtgga tcacgtcgat gaccttgctg ttgcgcaggt cagggcagtc ttccttgaag
     5101 gtcatgccca gcacgacgat gtcggcatct ttcacagcct gaccggatgc gatcaggcgc
     5161 ttgacggtct gctccgccac gtatttgccc atgctgtcgt tgatgcgtcg gccagcgagg
     5221 atgacctgcg gctggtagcc gagcatctcc gccttgtggg tcaggtagta cggatcgacg
     5281 ccgatgcagt ggccaccgac cagacccggg cggaacggca ggaaattcca tttggtgcca
     5341 gccgcttgca gcacttcgat cgtgtcgatg cccatgcgat tgaagatcag cgagagttcg
     5401 ttcatcaaag cgatgttcag atcgcgctgc gtgttctcga tgaccttggc tgcctcggcc
     5461 acgcggatgc tgctcgccgg gtacacgcct gcttcgatga tcccggcata aagatctttc
     5521 acctgcgcca gcgtttccgg ggtgtcgcct gaaaccagct tgacgatctt cgtcagtgtg
     5581 cgggtcttgt cgccagggtt gatgcgctcc ggcgagtagc cgatgttgaa atcctgtttc
     5641 cacttgaagc cggaatactt ctccaggacg ggcacgcaca cttcttcggt ggcccctgga
     5701 tagacggtcg attcgtaaac cacgatcgcg ccacgcttca tgtggcgccc cacggtttcg
     5761 gagctgccga ccagcggctg gaaatcagga cgccgcgcgt cgtcgatcgg tgtggggacc
     5821 gcgacgatga tgaagtccgc ctcggccagc gcagcaccgt ccgtggtgca tgtcagcagg
     5881 gtggccgcac ggagttgttc ggtggacacc tcgcccgtcg ggtcttcaaa gcgccgatag
     5941 gcggccacct tgcgttcaga caggtccaca ccgatcgtcg tgaatcgctt gccgaactcg
     6001 acggcgaggg gcagtccgac gtagccgagg ccgacgacgg cgacaacagg ggtgcgagcg
     6061 ttcatgtttc agcggggttg acaaacatca aatgagtttc gcacgcggtg attcggccgg
     6121 aaccacgaga ttcgtgaggt ggttcgtgtt cctgacgcat ttgcttgcaa ccggatcgcc
     6181 gcaagccaag ccagtcatgc gtctcgcccg gggcgattcg caccgcgcag catgcgaccg
     6241 atccgcgtga tcacagattg ccagcgactg cgcggcaaca cggaggaggc cactgcagaa
     6301 ttcgtcggtg gcacctcttc cggggcgcga gcgctcggtt ctgtcgccgc ctgtcctgca
     6361 gaaaacggat cgtccgagac ttctgtcacc gtgcgcgaga gggtcgattc gatcaaggcg
     6421 tacacgtcct cggccttgac ctccgaacgc ggctgcaccg ggcgcttgac cttcggaggg
     6481 cggatcttcc gtttcggctg tgtcacctct gcccgctccg ccttgggcgg gtcagccaga
     6541 gccggggggg agaccatgac ggcctcggtc aggaccggcg gcgcaggctg ggcgacgtgc
     6601 ctgacggcat ccgggggttg agtcgccttc ggcggcctga gcgtcggagc cggccgcgtc
     6661 agtttcaacg aaaagtcatc gaacccggca atgcggttgc gcaggtggcc gaggcgcgga
     6721 cgggcgtcct catccacggc aaccagaaaa ctcactggca cttttgcccg cagcactgca
     6781 tcgacccaat cgctagcgaa ccgggcgttt ccggcgtgat ctggcggggc gcacaggaat
     6841 gcctcgaagc ggtcgagcag gatgatgaag ctcgcatcaa gccgggtgcc gagcgcttcc
     6901 agcgtgtcgc ccagacgctg ccacggagcc gagcgctcag cctcgcacgt gttggcaacc
     6961 tgatggatgc gggccagcag cgcgggcagg ggatcgtccg accaatcgtc gaattgaacg
     7021 acgaactcgc gtgtgcacgc tgcgttatga acagcagggc gtttgcgtcg atcggcagga
     7081 ggagctgcca cgccggtttc gcgggcatcc gccggcatga accgatcctc tgcccgccgg
     7141 cccagcaagg gcatcagcgc agaggtgagg aacgatgact tttccgtccc ttcgtcgcag
     7201 taaagcaagg ccaggcgaga ggttcgcagc agtgagacca gcaggcgtgc ctcccctgca
     7261 tgcgcctgcc acacggggtc gatgtcggat gcttgcgcag tagtcacgcc atcttcttct
     7321 ttgcgattcg gctgttcccg aggcaacacc ggctgctaca ggaagaatcg caaggccagc
     7381 tcgatggccc gccgctccgg gacgctccca tagacggatg ctgcgttgcc cgagtagagc
     7441 tgccactgaa gtgcgagttc ggcgctgtcc cagtggtagc gcgcttcgat ccactgatgg
     7501 cggctgtggg tctcagagtc gcgtcgcacg aatgcagaca gatcgaaccg ccgcaccagc
     7561 aggtccttcc agttggcata gaagaacaat gcgttgcgca ctggaaggtc ctggagagtt
     7621 ggtgcggtgc ccagcacgcg caagcgagcc gaaggatcga gacccggcag cgctttccac
     7681 tgggatcgat cgggggccgc actgttgtac tgtgcctcgg cggtgaggct gaggttcagc
     7741 ggcgtggtgt aggtcacgcc cactgcagcc cgttcctggt ggcgtcgcgc ctgatcgagc
     7801 tcgagcgctt gcgccaccag cgatcggcct ttgccggtgg tgaactcccc gaacacgacc
     7861 gccgagtcgc tgatcagggc ggagacgttc aatccgacct gcatccgtgt gttgtcgccg
     7921 ccgtacagca gcacttccgt gttcagcttg tcgctgaact tggtgctgcc ggccagcagc
     7981 cagcggttgc gtggattcgt ggcaccgacg ttgagcgaga aggtgccggg attgggctca
     8041 cgccccagct tgggcgagta ggtcgcggtg atggatcctg cactccacaa ctgttgccct
     8101 tgcagtacca ccgtgccctg gcggttctcg cgcagtactg cgggatccgg cgagacgatc
     8161 gagcgaagcg cgccttcctt gaaccagtcg gtcgggttgt acgcgaaggc tgccccgtgg
     8221 cgaacgttga cccgacccac atcgaggatc tgctcgtctg tgcgggccca gctcaggtac
     8281 gcctcgcgca gcgtgttcac atcatgagcg cgggcggatc cgttcttgtg cagcagatcc
     8341 aggcgatcgg aaaacacggc acgcaggccc ggtgcgatgc tgctgtcgaa gcgcacgtcg
     8401 attgacccac gcgtggtctc gaaattcgag tccgaaccgc cgactccggt gcgaacgccg
     8461 gcgccttcga gaaagatgcg ccacgccttt gcggcctggg gcgcggtcgt gggcgccttg
     8521 tcggcgagcg acagggcatc gagatcatcg ggagcagcgt ccggcggact ggctgcttgt
     8581 gccacagtgg agacgagcag cgccaattgc agcagaaggg tatcgagtcg catacttatt
     8641 ccggtttgaa gcgtggcagg taatcgcgtt gcagccagga ctcgggaacc tcccgccagg
     8701 cgtaatcgct gtagcgcatc accgtcaccc aggctgggtc gagaccatcg atgatgacga
     8761 cttctgtcgg tcgctctcgg ccgaggtgtg gctgaaactt tcggtagtag gctgtcttga
     8821 gcaggtgtcc gctttcagcg aagaagcgtg ccttgaccgg acgggacgat gtagtttcga
     8881 tccacatttc tacacggtgg tacgtcacgt ctggagcagc tgcagccagg gcgagcttgt
     8941 agcaacggcg catcacacgc tcgccgtcag tcaggtcctc ctcgctttcc aaggttgccg
     9001 cgtagtcctt cgccaggttg acagtgacca catctccgtt cgacgcctga cccagtaatc
     9061 tttgttgcgg cgacaaacgg atgcttgcct gactcgccgg gtcgaagaac cacaggtcgt
     9121 tgccgttttt caacatgagc ttgttggcat ctcgctgcgg cgccacgaag cgcagcaaac
     9181 tgcggtattg cccgctggac gcgtctgcct tcgagtacac ggtcagcgtg ttgttgtcgg
     9241 tctgcttcgc gctgcggtac tcgagcaagg tcaccgtcac agcgaacgat ttggacggat
     9301 tacgaactgc gtcgctggcc gcgaggatgg cctgcgcgtc cggggcggcg agggcgggcg
     9361 agctcagccc caggccgacc caggccagac tgactatcaa ggcaagggtt gtattcatca
     9421 agtttccctt ccttgcaccc ggactaaaca tggcgcaagg catcgacgat cgtcatacgc
     9481 gctgcacggg ctgcgggcag gatggcagaa agcgctgcaa cgacgacgag tccgacggca
     9541 ctggcaatga tcatccagtg tgctcccgcc aggttgacgg ccagcggcac gggttcgttg
     9601 cggccaggtg gaacccaggt caggcccagg ctgttgatga cccatgccag ggctgcggcg
     9661 atgccgattc ccagcactgc gccaaagcag cccagaacga tcccctcgct gacgaacatc
     9721 gcccggatgc cgctctgcct cagacccatg gcgcgcagcg tgccgatctc cgaggtgcgt
     9781 tcgaccacgg ccatgctcat tgtgttgctg acggtaaaca ggacgatcga tcccatcaac
     9841 atcgagatga aaccgaagat ggctgcgaac atcgccagcg tttgcccgta gaacgggttc
     9901 aaggtttcgt aatcgagaac agccagaggt tcaccccgaa gctcggtcgt caaaagggtc
     9961 tcgaggcgag cgcgtacttc gggaatctgt gcggtgtgct tcagttgaat cgcaatcgca
    10021 gtcgcttgtg gcggcgctgc tccgaaaacc agcttctgtg cttgcgacaa atgcagtgcg
    10081 atgtagatgt cgtccacctc cttgacgccc tgaaactcag ctgcgagcac gttgacgcca
    10141 gccacgttgg gagcgccgcg agcgttggcc gcgagaattt cgatgcgtgg tccagcatcg
    10201 ggtttcgtta cggcattttt cgtcgtaccc agcatggcca agtcggatat gtcgtccgga
    10261 agcgccgcgc cgtcgaccgc tttcttggaa tcttcagggg cgcaatcctc aactttcaga
    10321 ggcgcgcaca aatggagaac ccgtgccaca ccggtaccga tgacagccgt atccggcggg
    10381 gtaccggaca gggagagatg tctcgacgac agatcacgct gcccatagtc attccattca
    10441 cgcatctggt tctgatcttc aacgactgtt ccgttgatga agacggtgcg tgacacgctc
    10501 gctgcaaagt ttccggcgat agcaccgaat tgcagcgtgg gagtgatcac tgcaagcatc
    10561 ggcgcgatca ccgggtcctt cctgactgca gcgatgacgc gctcgtaatt ttgaatcccg
    10621 taggctgagg gattgccgct gccgaaacgg aagtaatcct tgtgctggat ctgcaggtgc
    10681 cccgtactgg tgacgaaatt ggtttgcagg ctgtagttga tgtctctgat gtacccgcca
    10741 aagagaagaa ctgccgtcag gcccagaatc atcgcgatca aggtcatgag cgagcgccgc
    10801 cgattgcgca gcaggttccg ccatgccagc gacagcgtgt tcatgtacgt gattccgcca
    10861 tggcgaggcg ccgctcgatc gatgtgatgc ggccatcatg gatgtgcacg gcgtcatcag
    10921 cagcttcgag caccttcggg tcgtgcgagg agaaaatgaa cgaaacgcga tgctttttct
    10981 gcatgcttcg catcagttcg atgatctcga cgccggtctt gctgtccagg ttggccgtgg
    11041 gctcgtcggc cagcacgagt tgcggcctca gcgccagcgc gcgggcgatc gcgacgcgct
    11101 gacgctgtcc gcccgacaac tggccgggcc ggttgcccgc gcgatcggcc aggcccactg
    11161 cctccagcag tttcgcgacc cgagcgcgcc gcttgtcggc tggcatgccg gtcagcacca
    11221 gtgggtactc gacgttctcg aaggcggtca gcacgggcag caggttgaaa ttctggaaga
    11281 tgaatccaag ctgatgcgcc cggaagtccg acagggcgtc gtcctccatc gtctgcaccg
    11341 gccggcctcc gaccatgatc gtccccttgt cgggccggtc gatgcagccg atcaggttga
    11401 gcaacgttgt cttgccgctg cccgacggtc cggacaggac cgtgaatcgg ttcggaagga
    11461 tcaccagatt gatgtcggtc agcgccggga cgtcgacgga gtcgaggcgg taggtgcgcg
    11521 aaacaccctt cagcacgacc gggtgcgcca ggttcctgac gcgttcgccc ggcatcgttt
    11581 caagcggcgg catgcagtgc agtcctcaca ggcgattcat ggatatcagc atgtccagct
    11641 gcagtcctgg gtcggggcga tgccgattcg agcatcagct gcgccagcac cggacaaatt
    11701 cgcatggtgc cgatgctaac ggcagggcat ggccggaaaa gcagttgatt tgcagcgttt
    11761 tacaggctgc cgtgcatctg cggcaaacag gaaatcagct ccgctggcct gatcctgatc
    11821 atgtggcagt catcggccgg cggggactcg ctccctgccg gtcaacagat tgccggtcgg
    11881 tacccggcct caggtccgtg ccacgcgcac ggtaaggcag ttgcggccgg cgttgcgttc
    11941 gtaagcaacc gacctcgcgt acttgcgaac cagcatcaat cccagaccgc ccgggaccgc
    12001 cttttcgatg gaggtgggca gcttcggcgc cggcgcaagc aggggatcga aggccatccc
    12061 gtcgtcctcg aaatgcatca cgatgtcctc gggctcgacc tgcacactca tcgtgatcgg
    12121 gtgcacttcg tcgtctggaa acgcatgcca gatcacattc atcagcgttt cctccaggat
    12181 caactcgacc ttgaacaaca gttgctggct cgctccatgt ggagccaggt gcctcagcac
    12241 ggccagtcgt gtctggtcga atccttcgcg atcgttggcc agcgtcagca gcaactgtgc
    12301 gccgtcgcct gcctccgagt gcgatgcagc tcccgccatg gtggattccg ggaattcagg
    12361 cttccgatgt gccgcagcgc tcagccctgg agtcgctcca gagcctgcgt ctcattggcc
    12421 gcgatcggga tgatctggtc gagggccacg tgttcaagca cgctgtggac gaggggctgg
    12481 gcgccgaaca gcaccatctt tgcgcccttc aggtgaagcc cacgcgcgct tgcaatgaac
    12541 atgcgtatac ccatcgaagc aaggaaatca acgcccgaca gatcgatcac ggcgttgtgc
    12601 gcccgggaga ccaccgccgc ggtgaacttc acctcaatgc ggtcgatgcc gggtgaatcg
    12661 agtcggccgg agagccgaat gcaggtcacg cctccatcca gctctgtcac atccatgcct
    12721 gtgtccataa cgggttccct tcaaagatac gtgttcagcc tgcggctcga tcgctgtcgc
    12781 gccgcctttg cgtgacgaga atatggcggt cgcgctacac gaacgtgaca accagcattg
    12841 aaaagtcatc atccagtgcg ccagggcggg cgacgtcctg cacggcgcga tagagtctct
    12901 cgggttcggc gacctcggcc gactcgggac gccgcagcag cggcaggaaa tcctgcagac
    12961 cccactgcgt gccttccttg gtcacgactt cgaacacacc gtcgctgaac acgtagagca
    13021 cgctgccggg catcacctcc acgctgctgg agatgaactt ggcttcgggc atcgcgccga
    13081 tcatcaggtt gcgtgtctgc agcggaatca gttcctggat cgacggctgc gccaggtacg
    13141 acggatggtg tccggccgac gagtagcgca gcaatcgggt cttgggctcg tagacgccgt
    13201 accagatcga gaaatacatc ccgccatggc tttccatctg gaacgtcgtg ttcagactgt
    13261 ggagcacctg ggccgggtcg cggaaatcgg tgttcggcaa cgcgcgttgc cgcagcacgt
    13321 tcatgaccga cacgctgtgc atcgccgcgc ccgcaccgtg accggacaca tccacgacgt
    13381 agcctgcaaa ggtgtcttcg tcgagttgct ggtagccgaa ggcatcgccg ccgagcgtcg
    13441 tggaaggcag gaagaaccag tcggttcgta ccggaccctc gcgtatcggc gcgggcagca
    13501 aggccttcac atagctgctg gccttttcga gatcgcggtc gagttcgagc gactgctcga
    13561 gctcgctcgg gagacggtac tcgtgcttca acacgtggcc gccgagctgc agcagcccgc
    13621 cgttgggaag acggatcgat ccgatcaggc gcttaccttc gacgaaggtg ccgttggtcg
    13681 actgcaggtc ggtgacggtg gcatcggctt cgtcaggggc tgcgctgatc tggcagtgct
    13741 ttcctgaaac ctcggcatcg ggcagcacga aatcgcacac catcttgcgt cccaccgtga
    13801 tcgggtggtc ggccgcgaaa cgcaggcgac gcccggtctg gctgccctgg atcgccacca
    13861 ggaaatgctc gcgcttctcc gctgcgacct ttggtgcgat cgtggatcgg gtgatggtca
    13921 ggatcgtcaa atcctgattc gaatcgttct ctgacgggag ggacttgttc aaggttgcgt
    13981 ctcgggaggc tgcgagatgt gcatgcgggc tgctctcgca tctcggcagg gtgttgcaac
    14041 accgatgcca gaatcgcgaa tgtaagagat tgttggcatc ggttgtgcgt tgctttgcgg
    14101 tgcttttctg ccttggccgg ggcaggctcg gtcgattaca acaacaacag gagtcatttc
    14161 gatgtccgca ccctctgccg gggatggtgg tgccgcgacc cagccggtgt cccgtgacga
    14221 agacgatgca ccaactcgga tcgcaacgac ccagcccgtc gtgatgccct ccgctggtgc
    14281 gcgagaggcg gacagctcgg ccaccttgcc tggcacctcg ctggctgacg gcggcgtgct
    14341 gcagcaggtc ggccgctacc ggatacacga acgcctgggc cggggcggca tggccacggt
    14401 gttcaaggcc cacgatccag gcatcggacg tgatgtcgcg atcaagttcc tgcacgcatc
    14461 gctgtgcgaa gagcccgaat accgtgcacg cttcctcagc gaggcgcgcg ctgccggcgg
    14521 tttgtcgcat ccgaacatcg tcaccgtgca cgatgttggt gaaatcgacg gccggcccta
    14581 catggcgatg gaactgctgg acggcgaaac actgagcgat ctgctggcca acaatccgcc
    14641 gctgcgtgtg cgcgacgtgg tcgtcatggg catccagctg gcccgggcac tcgactacgc
    14701 tcattcgcgg gggatcgtcc atcgagacat caagcccggc aacatcatgc aggtgaaggg
    14761 cacccagacg gtcaaggtca ccgatttcgg tatcgcccac gtcgaatcgt cgggttccga
    14821 acaacggacg cacctcggcg acatcctcgg cacgccacag tacatgtcgc ccgagcaggc
    14881 caagggggag cgtctcgatg gccgctccga cctgttctcc gcgggcatcg tgctctacca
    14941 gatgctgacc gggcagcgcc cattccaggg cgacagcctg gtgtcgctgg cgatgcagat
    15001 cgcgacagcg gagccgaagc cgatcgagca attgcgtccc gaagtcccgg ctgccctgcg
    15061 ccgtgtcgtg tcccggtcgc tggccaagtt gccggaccgg cgctgggcca gcggcaagga
    15121 actcgcagaa gccctgacga gggtgctcgc cgaaattgac gcggccgaac tcgagaagag
    15181 ccgccagcgc atcgttccgt tgcgcgtcaa gtgggccgcc acgatggcgc tcatcgtggc
    15241 ggtggtgatg gcgttggccg ccaccgtcat cacgcagcgc cagtacgctg ccatgttcag
    15301 ccaggccacc gattacggtg cgtcgctggc gcggttcatc gcagcgcaga acgcggtatc
    15361 ggctctctct gaagactggg tggcggtcga ggtgtcgatc caggaagtga tgaaggcgcg
    15421 ggacttccag agcatcgcgg tgatcgaccg cgaaggcatg gtccgggcca ccagcgcggg
    15481 cagcaccggc ctggcctatc gggcccccgc cagtgaagcg ctcggggagc gaaagggcgg
    15541 cgtcacggtg acgcgtttca cggccgacgg ggaatcggta ctcggattcg aggcgccgat
    15601 cacctttcag gacaagcgtg tcggcagcgt ctccctcggc ctgcccgaaa agccgctgat
    15661 cgccgtcgcg cgcctgtcca tcgggctgat ggtcgctctg gtcgtgatca ccgtgcttgc
    15721 ggtcgcggtg gcgatgttct ttgttgccaa ctggtttgca cagccgatca agttgctcgg
    15781 agaatccatg caggagatcg ggaaggggcg gttcgaccat cgcattgccg agcagcgcaa
    15841 ggacgagttc gggcagctct atgcgacctt cgatcagatg gcccaggcgc tggccgaccg
    15901 gcaatcgggt gcagtcgtcg ggccgcctac gccgaccccg ctgccgaccc gggctggtcc
    15961 gggtcccgct tcgaacaagg aatgagatcg tgatgagtgc gaggcaagtg ttgcttgttg
    16021 cggcagcctg ggtggtcgtc tggctggccg gctgcgccag tgctccgccg cccagcgtga
    16081 ccgcgcctgc caccgaggtc cgcagcggca acggcaatgg cgatgtgctg ggacgcagcg
    16141 atcgtttcgt catctaccag ccggacaacg acgacacctt gcgctcgatt gcggggcagt
    16201 tcctcggctc gcccgatcgc gactgggtca tcggcgactt caaccggttg agccggccag
    16261 tcgccggcca acctctggtg atacctattg ccccgatcaa ccaggttggc gtgcaaggcg
    16321 accgctacca gacggtgccc atcctctgtt accaccggtt cggtggtaac agcagcaaga
    16381 tgtcggtgtc gtcggaaaat ttcgctgccc agctcgactg gctggcgcgc aacgattacc
    16441 gcgtgattcc gttgtcgcag ctgctcggct tccttcaggg gagagaggcg ctgcccaagc
    16501 gctcggtggt catcacgatg gacgacggct acgaatcggt gcaccggcat gcgcttccgc
    16561 aactgcgcaa gcacgggttt ccggcgaccc tcttcgtcta caccgatttc atcggtgccg
    16621 gcgacgggct gagctggaac cagctcgcgg agctgcaggc ttccgggctg gtcgacatcc
    16681 aggcgcattc gaagagccac cgcaacctga tcgagcgcac cacgggcgag acggacgagc
    16741 gttatcgcca aagcatcgac gccgaagttc gcgtgcctcg cgagttgatc gagcgccgct
    16801 tgccgcagca tcaagtgcgc catttcgcgt tcccgtacgg ggacgccaat gaatcggtgc
    16861 tggacgcgct gttacgtcag caataccagt tggcggtgac ggtctacccg ggcggcaatg
    16921 ccttcttcgc gacgccgtac atgctgcgcc gcacgatgat cttcggcgac atcgacatcg
    16981 aggcattcaa ggccaagctg cagatcagcc gagccacccc cgcgccatga ggagcctgcc
    17041 ggcgctgctc ctgggcgggt gtgtggcgac cttgctcggt tgcagcagca cggcgccgcc
    17101 ccccgccgcc aggtcggtgg catctcccca gccgacggcc gctccctcgc ggtccggctt
    17161 cgagcgggaa taccgggaac gcgcagcgaa cgccgcgcgg cagggcaggt tggcagaggc
    17221 cgcgctggcg tgggaggtgc tcaccgttct gcgacccgat gtcaccgagt accgcgaggc
    17281 cctcgccgac acgcgtcgcc agatcgacgc tgcggtggcc gatcgcctgc cccgcgcggc
    17341 ccaggcgcaa cgccgcggcg acctcgatgc cgcaagccag ctctacctgt ccgttcttgc
    17401 cctgcagccc gaacacgcga cggctgccga tgccttgcgc gccgtcgaac gcgagcgggt
    17461 caagcgtcag cagctcggga gatcgtcccg gacgatgctg acccgccgtg cagccaccga
    17521 cgccgaggtc atggccgaca aggcggctgt ggccggcggt gccgaccgca acgaactcga
    17581 gcatgtggcg atgctcgccc gccaagggga gctcgacagc gccatcggct tgctggagcg
    17641 tcgcgttgcc ggcagccgca acgatcgcgc ggcgcgtgaa ctgctggctg acctctacga
    17701 acgcaaggcg gaagaagcag tgacggctcg cgattccgcg gcggccatcg tgttgctgga
    17761 gaagagcgtg cgtctcgatc cgtccgatac acgggctgct gagcgactca agcaacttcg
    17821 cgcctctgcc gcgacgcgac cggccgattc gaagagccgt tgagcctggc ctgcgcagcg
    17881 gcgctgcgca ccgcggctac ttcagggact tgatcttttc cttgagcgcc cgagccttct
    17941 gctgctcgag gcgggcggtg tcgttgccgg gttcgagttg cagcacgcgg tcccactggg
    18001 caatcgatcc gtcgagatcc tgcttggcga acgctccgcg cgctgccagc gtgaagcgcg
    18061 tgaccaggcg gcggcgcacc tgttcggcct tggcaagcgc gtcgttgctc agttcaccgc
    18121 ctgtggctgt ccggtaatcg ctgtaggcac ggtccaggtc tccgcggcgt tcggcggctt
    18181 cagcgcttgt cagggccttc tcgcctgcac tcggttcgtt ggtgaccggg gcggccggcg
    18241 cgaccgtcgt gacagggggc gtggggttgg ttggagccac ggccttgtca cgacgtgctt
    18301 cgttctccag caccccgggc ggcggcgcct tgccaggaat gcggatcacc tggccgcccg
    18361 cgacctgacg tggaatcttg atgtcgttgt agcgcgccag gatgtagaag gagtagatgt
    18421 cgccgaggaa gcgacccgcg atgcgcgaca gcgtgtcgct ggagcgcacc gtgtacgaaa
    18481 acgactcccg accgagtacc gcgacaggat ccgcggaaat ctggcgcatc aggttctgcg
    18541 cgagcttgtt gcccgggtcg ctggcgagca cacgctgaag atcggccttg gcctgctcct
    18601 cgttgccggc ttcaagcaga tccgcgctgg cgagcgccat cttctgggct tgctgcagcg
    18661 tcagcggagg aggcggtggc ggggccggag gcgtctcgac cggtgccggt tcggtgactg
    18721 cggggaccgg gacgggagcc ttggcggggg gagtgccgca tgaggtcacc agaacggcgg
    18781 tggagcacaa caggatgaga cggatcgcaa gcatggaatg gggtggcggt ggtggtggtc
    18841 cgctgggtta ccgtcgcctt atggaagccg cagattatcg acggtgcgcc gaatcacctg
    18901 caagcacatc cggcagttcg cccgccgctg cgcgctgcag tgcggggcag gaaagcctgt
    18961 tccgagccct gtggcgtctg gcgctgggcg ccagctgcct gctgtcgtgt gtctcggcac
    19021 acgctcaagc cgccgccggc ttcgactcgt gcgtccgcag cttgcgggcc cagttgccga
    19081 agcagccgcc ggtgagcgcg gagactttcg atcgctatac gcgcggcgcc gaagacctgc
    19141 gcccgctgat cgatcgctcc acggcaacac agcccgaatt caagttgccg gtgtgggatt
    19201 acctggcccg gctggtcgac gcgcagcgcg aggcggacgg ccgggcggtc ctcgatcgag
    19261 aggccgtcgc gctggccgcc atcgagcgca ggcacgcggt cgatgcagcg accgcggtgg
    19321 ccgtgttcgg cgtcgagacg gactatgggc gggtgccggg gcgctatcct gtggtcgacg
    19381 ccacgttgag ccgcgcctgc ttgaatctcg ccagccgcga gcgaaaggcg cacttctatg
    19441 cggcgctctg gttgcttcag gaggggctgg tgcgcccgga gaccttccgc ggctcgtggg
    19501 ccggcgcgtt cggcctgacg cagttcatgc ccggcacctt cctgcagaac atggatgacg
    19561 gcgatgattc cgggagcatc gacatcgtcg acagcgtgcc cgacgcgctg gccaccactg
    19621 cgcgctattt caaggggctg ggctggacgg ccggcctgcc gtggggcatc gaggtcagcg
    19681 tgccgcggga acaggcgctg gcctggaatg cactggagcg tgaccatgct tgcctggagg
    19741 cggagatgcc ggcaggcctg tgtcgtggcc tggatcagtg ggccgccgca gggtttcggc
    19801 gcatcgacgg cggggcgctg cttccggagg gccccggcag tgaatcgccg ctgcgcccgg
    19861 acacgccagc cgcgcttttg atgccggggg gaatcgacgg gccggcctgg ctcgtcaccc
    19921 gcaactacca ggctgcgtgg cgctacaacc gggcggatgc ctatgcgctg gcgatcggcc
    19981 tgctggccga tcggctgcgc ggcgatccgc cgatgcgtgc cgcgtggccg accgatgacg
    20041 ccgccctgtc ccgcgccgaa ttcgccgaac tgcagatgct gctgaggcag cgtggccatt
    20101 gcgaagtgac tgccgacggc catgacgggc cccgcacccg cagtgccatc gaggccgagg
    20161 agcgctggct gggcaacaac ccgaccgggc gcgccggcgc caggctgctc gcggccttgc
    20221 gcgcggcgac cccgatggag ccggtcgagt cccccgcctg cgccgacgcc ggccagcgtt
    20281 gaatcgcgac gaagcgggcg gccgctcgcg cgtcagtcgc cgccccggta catgccggcg
    20341 tacaggccgg gcgtcgaatc cgcgatgtcg ctggcgccgg ccacccgggt gaagaattcc
    20401 ttcgcgccga cggctccgac gatcgcatag ccgtaaccgg ctgcgcgcat gtcggccagt
    20461 gccgcacgca acagcgcggc cccggtgccc gagcgcctgg ctgcctcgtc aaccccgatc
    20521 gggccgacgt accccctggc cgtggcgtcg tagcaggcga agccgatcaa ccgggcgccc
    20581 tgcatcgcga tgtacaggct gaccggccgg ttctggaggg caacgcgcgc ctcgctggcc
    20641 cagcgcgaac cgaacgcctg ctgaatccac gccacgacgg cctcgtgttc agggccgatc
    20701 ggcttgcgca ccagtgccgc tggcgacccg gccgacgact caccggccgc accgggcggc
    20761 aggtcgtaca gcttgatcag catgtctgtc atcgcgccca ttcttgcagg ccggcgacgc
    20821 ttcgtggagg gcagatggcg cttttggcat acgtcttgct gaaacgattc ctgtccctca
    20881 aagaccgtgc ttcaagaaca ggccatgccg caaaaccaga ccgatggatc catgatcagc
    20941 gacctgctgc agtcgatcca tgcccgctac agcagccttc acgatggcaa cgtcgccacg
    21001 tacataccgg cgctggccca ggccaacccg gatcagttcg gcgtgtgcgt cgtctcgacg
    21061 cgaggcgaca tcttcgaagt cggtgacagc cgcaccgagt tcacgatcca gtcctgttcg
    21121 aaggcgctgg tctacgcgca tgccttgtcc acccacgggc gcaccaaggt gctcgagcgg
    21181 gtcggcatcg agccgagcgg cgacgcgttc aattccatcg cgctcgacgc cagcaaccgc
    21241 gcgttcaacc cgatggtcaa tgccggcgcg atcaccaccg ccggattcat cgaaggcgcc
    21301 gacttcgccg agcgccgtgc cgcgctgctg cgctgtttca gcgccgcggc cggccgcgaa
    21361 ctcgaagtcg atgaaaccgt ctacgcgtcg gagcgcgaga ccggtcaccg caaccgcgcg
    21421 ctggcctggc tggtcaccaa cttcgaccgc atggatccgg cgaccaacat ggagacgctg
    21481 gacctgtact tcctgcagtg ctcggtgcgc gtgaatgcgc gcgacctcgc cgtgatgggg
    21541 gcgacgctgg cgaatctcgg cgtcaatccg ctgaccaagg aaaccgtgtt cgacatggcg
    21601 agcatgcagg acgtgctgtc ggtgatggcc acctgcggca tgtacgacta cgccggcgag
    21661 tgggtgttcc gcgtcggcat cccggccaag agtggtgtcg gtggcggcat cctcggcgtc
    21721 gtcaaccggc agctcggcat cggaacctat tcgccccggc tcgacgcccg cggcaacagc
    21781 gtgcgcggca tccgcgcctt ctgcgacctc tcggacgacc tgggcctgca tgccttcaac
    21841 ttcatgaaca gtggatcgac cttcctgaag cacctggtct gaagctcgcc gatttggcgg
    21901 ccggaacgat cgatcgacgt cagttcgaat cggccatgcg cagccggcgg gcggcttcct
    21961 cggcgcgcgc atcgtcgatc tcgcgcatca cgccgctcag gtcgaccgct ccggtctggt
    22021 gctcgtagcg tccggtcaac accacttccg gcgtcagcat gccgcgcacg tacaggtccc
    22081 agatctcggc gccgtattcg gtggccagca gttcgggcgc gtagcggccg aagtagctgc
    22141 gcaggttggc cacgtcgcgc agcagcatgc gtggcgcatg gttgttgccg gcggcgtcga
    22201 tggcctgcgg caggtcgatg atcactgggc cgtcggccgc cagcaggatg ttgaactccg
    22261 acaggtcgcc gtgcacgacg ccggcgcaca gcatgcggac cacctcggtc agcaggctcg
    22321 catggtgcgt gcgcgcctcg tcgggcgtga aggccacgtc gttgagccgg ggtgcggcgt
    22381 cgccgtgggc atccgtcacc agttccatca gcagcacgcc atcgtggaag ttgtagggct
    22441 tcggcacccg cacgcccgcg gcggccagcc ggtacagcgc atcgacctcg gcactctgcc
    22501 aggccgcttc ctgcgattcg cggccatatc gcgtgccctt ggccatcgcg cgcgcctggc
    22561 gcgagttctt gaccttgcgg ttctcggtgt agtcgacggc ctgccggaag ctgcgctgcg
    22621 tggcctcctt gtagaccttg gcgcagcgcg tctcgtcgcc gcagcgaacg acgaacacca
    22681 tggcttcctt gccgctcatc agctgccgca cgacgctgtc gatcaggcct tcgtcgacca
    22741 ggggctgcaa tcgcgggggt gctttcatcg gtggattctg cgttgtccgc cccaaccact
    22801 gcgaaacggg cctgccggag caatggtgca gtccccgagg ccgtgacaat tttccggggc
    22861 caggccgttc tatcggtcgc cccgattgat aagggtcaat acctataaaa aggcgctgtg
    22921 tatcctgtag gagtcactca acgagttcaa cgaaagcgcc catgaaagcc catctcctct
    22981 ccatcgcagc cctgctggtc gcttcatccg cggcctgggc cgatcctgcc aacgacaagg
    23041 ccatgctgga gttggccacc cacagcggct gcatgacctg ccatcagatc gagagcggtg
    23101 cgaagggccc cgacggtctc gcgccggtcg gcccgccctg gagcgatgtg gccgccaagt
    23161 acaagggcac gaagggcgcg gaaaagcaac tgactcacat cgtgatgacc ggatccaacc
    23221 cctatgccag tcactggaag ggcaaggtca gcggcctggc catgccgccg aacgaggtgg
    23281 cgatcaagga agccgacgcg cgcaggctgg tccactggat cctcaagctc gacagcaagg
    23341 gctgaccagc ctttcggctg gtgcggctca gctgccgcgc cagcccgcac cgtcgcgccg
    23401 cgcgcggccc acggcttcga gcgtgtcgag gatgcggggc aggcctttct tccacgggcc
    23461 cttgcctttg aagctggctt cgatggtggc cagcggcagc ggtgcggggc tggcgctgag
    23521 caactgagcc accgcgcgca cctgctcggg cagggtcggc ggccagggct gggtggtggc
    23581 agcagcggcc tggggtgcgg ctgcgccgcc ggcgtcgcct tctgcaggcg cttcgaccac
    23641 ttcggtttcg gccaggtctg ctctcgcggc ggcctcggct tgggactcca cgccgtcgag
    23701 tcccgtttgt tcgggctggg gcgcaacgga agcacccgcc atccgccgcg ccgggtcctg
    23761 gaattcgggg cgcagccagc gcacggtgcc cccgggcgga ctgcccggca acgtgcccgc
    23821 ttcctcggcc gcgcgcttcg cgttcagcgc caccagccgc tccagcagcg cttcggtcca
    23881 cgcggcgcgg gccgcttcgt ccgcccacgg cacggcgccc aggtcgctcc agccgtagg
//
LOCUS       DAOORV010000048        23706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2606_length_23706_cov_7.411949, whole genome shotgun sequence.
ACCESSION   DAOORV010000048 DAOORV010000000
VERSION     DAOORV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_23706_cov_7.411949"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1128
                     /locus_tag="PKV56_08380"
     CDS             40..1128
                     /locus_tag="PKV56_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOX67873.1"
                     /translation="MKTPHTLSLPRCALALFGAAALFGASAQAADGGQAYVSNQAGNV
                     SVIDLATLEVTREIDAYGKEPRGLGVTADGKLLITANRGDGNISVIDRATGNLLKNIA
                     IGKNPEFVRTRGHFAFVSFEPSSTGGPPPKPGSPEAMAAEKAKDDDDESPAYVAVVDL
                     KAGKVIRRIRGGMETEGIEFSADGKHILVTNEADDNVTVHSIASGKKVKTIDVKSYGD
                     RPRGIKRAPDGKSYVATIEHGNTLVVMDAKYNVVKSVPTGDTPYGLAFDPTGERLFVA
                     NSKSKNLQVYDAKTYETIKTIPTGDRCWHFSFTPDGSQILVACGRSNEVVVIDAKTLE
                     PVKRIEDKKLPWGLVTYPKAFGSLDQPE"
     gene            1167..2354
                     /locus_tag="PKV56_08385"
     CDS             1167..2354
                     /locus_tag="PKV56_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOX67874.1"
                     /translation="MTATRSPRHPRARPPAAVTADSEETILVLQGGGALGAYQGGVFE
                     ALSAVHHEPTWVAGISIGAINAALIAGNPPADRTARLREFWDLVSSALPTPPIVAGAR
                     AREALNEISATQVMQFGVPGFFQPRFPPAPFQPIGTLEAISFYDTTPLKQTLERLIDF
                     DRINAGPMRLSVGAVNVRTGNFAYFDSAKQRIDVRHIMASGALPPGFAPVEIDGEHFW
                     DGGLVSNTPLQYVLDQPGKRRRVVFQVDLFAAVGALPTTLPEVSEREKDIRFSSRTRL
                     TTTNELDRQVIAQAAQRLLAKLPPELRHDPDVLALSRVRCEHAVDVVHLIYRSKHYES
                     QSKDYEFSRLSMQEHWAAGQADMAHTLHDPRWLQRERHPHGVRVFDLTGDSPHLYNSE
                     TAS"
     gene            2351..3133
                     /locus_tag="PKV56_08390"
     CDS             2351..3133
                     /locus_tag="PKV56_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOX67875.1"
                     /translation="MKLQDKVCIITGAASGIGKEIAIVYAREGGKVVIADMNKVAAQA
                     TADEITKAGGTAMAVAMDVTSEEQVNAAVAEAVAAYGGVDVLVSNAGVQIVHPVEDFP
                     FADWKKMLAIHLDGAFLTTKACVPHMKASGKGGSIIYMGSVHSKEASVLKSAYVTAKH
                     GLMGLCKTVSKEGGKHGIRANVICPGFVRTPLVDKQIPEQAKALGISEDDVVKKVMLK
                     ETVDGEFTTVQDVADVALLFASFNSNALTGQSLVVSHGWFMQ"
     gene            complement(3214..4587)
                     /locus_tag="PKV56_08395"
     CDS             complement(3214..4587)
                     /locus_tag="PKV56_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX67876.1"
                     /translation="MATILIVDDEVTFARNAAKYLERFGHTVSTAATVAEGKALAETQ
                     KPEALVLDYRLPDGTGLEFIEHIRASDPAVHIILITGHGTIELAVDAMKAGANDLLTK
                     PVSLLELRERIDVLMQQQRDASRLRYFEARERSASQSILGQSPAIRELQQRIERMASI
                     DAEGMLPPILIGGETGTGKELVARACHYLSPRSAKPFIEINCAAMPPTLLETELFGHE
                     RGAFTDAKERKIGLLEAADGGTLFLDEVGDMDLSLQAKLLKVIEDGRFRRIGSVQEHQ
                     VCVRIVAATNHDLEARVAEGVFRADLYFRLRVLQLMVPPLRDREDDAVFLARHFCKLM
                     GQRYRREGVKLGASAEAAIAAYRWPGNVRELRNVIEQAVMLAASDTIEPRELMLSSAA
                     STSAAGSAAPAAPSTEAGSALDRRERELLIQAMAESNRNVSQAARALGISRDTLRYRL
                     EKHGLKE"
     gene            complement(4591..5943)
                     /locus_tag="PKV56_08400"
     CDS             complement(4591..5943)
                     /locus_tag="PKV56_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX67877.1"
                     /translation="MPPSQPAPFQLLRWFAGLSAVVIVLIAMANAWVVSRFLTSQMFK
                     REGEISRDFVQNILVSDGTLAYLRHTDDPQVVEHFKNTVTHLANMRDMLRANVYGIDR
                     TVVWSSDPTMVGRRFTDNDDLEHAMAGKLEVESGRISHEALSKREHIGLSPSVQFFVE
                     TYIPVVDPSDNQVVGVVELYKAPLALTEAIEEGERQVAIAALGGAVALFVSLFWLIRR
                     ADRTMREQHTKLLEAGTVAALGELASSVAHNIRNPLASIRSSAELGLELPPEQSADCS
                     RDIIREVDRISARITELLRLSNQGVQASQPVDVVSVLRNCVSDHKITFQRSRKTLSLE
                     SHIAKAVVQADSHLLQQVFDSLLSNAAEAIPPGERCDVTVRETDSRTVRIEVKDGGPG
                     MTPEVLAHIFRPFFTTKPQGLGLGLPLAKRIVEGLGGVLQIDSRPGEGTTVCIDLPRI
                     "
     gene            complement(6230..7117)
                     /locus_tag="PKV56_08405"
     CDS             complement(6230..7117)
                     /locus_tag="PKV56_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX67878.1"
                     /translation="MLLLALIAFWLWTPDKDRADLEARYLQSPSDIIEIAGVRLHVRD
                     SGPKDAPAVILIHGFGGSLHTWEPWAQTLAADHRVIRFDLPGSGLSSPDPTGDYTDAR
                     SLQLLRALMDDLGVARASVVGHSIGGRIAWNFAARHPERVDKLVLVAPDGFASPGFEY
                     GKAPEVPAAVRMMRFVLPKPLLRMSLAPAYGDPAFLSDALTARYHDLMLAPGSREALI
                     ARMQQSVLVDPRPLLRSIRAPTLLLWGEADAMIPLANSADYLKEMTGAKLVSLPGVGH
                     LPHEEAAQESITSVREFLK"
     gene            complement(7256..8203)
                     /locus_tag="PKV56_08410"
     CDS             complement(7256..8203)
                     /locus_tag="PKV56_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOX67879.1"
                     /translation="MNRFLKTMLAFTTAGLMAACGGGGSSSDPENIAVVAQAQGFNAL
                     LAAVNKAGVAGALTNENASLTVFAPTDAAFTQLANRLGFASAAAMVDALPAPVLANIL
                     TYHLLPDEKSAADLAAGGATQGTLYSFGGSPATLSLNTTSGVAITDAALTTARVTAAN
                     VNASNGIIHAIDKVLVPPGVLNIVQMAQVNPLFDTLVGAVVAADLQGTLSGTGPFTVF
                     APTNAVFADIASTVAGLTTPQLTTVLTYHVLGSQVLAADIPFGTPVNTVAGQPITINA
                     GTPPTITDQTATPAPILATDVRASNGVIHVIGKVLLPTL"
     gene            complement(8332..10788)
                     /locus_tag="PKV56_08415"
     CDS             complement(8332..10788)
                     /locus_tag="PKV56_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOX67880.1"
                     /translation="MIVYQPSGIVEGLKAALLRTSLKLLLKPVFSPRWSIGFQRRWLL
                     ALSRTTLIPKGVAIETAEVGGVPGEWLRSAQAPSAPQGVVLYLHGGAYCVGSPATHRS
                     LTARLALVTGLPVFSLDYRLAPEHPFPAGLDDAVAAFRALARGGQPVVLAGDSAGGGL
                     ALAAALALREGGSPMPAAMVLFSPWVDVALRDAPASEPRGEAMLSVAWAAACAAHYLR
                     DTSPDLPRVSPLFADLRGLPPTLIQAGTDELLHGQALQLEAALQAAGVDTRCEISAHR
                     WHVFQTSGGVLRSADEAIDRVARFVMRPLAKAAAANGPATSSHEVVVLGAGMSGLCAA
                     VKLKRAGIHDFVILEKQDGLGGTWWDNTYPGAHVDVPSPVYSFSFAPNPGWTRRFAGA
                     PEIQTYMQKVATRFGLLAHIRLGTRISAARFDDAAGHWHYDIERPEGARSTLQSRFFM
                     CSAGPLSQPRWPDIPGLDDFQGERLHSARWDHGYAMPGKRVAVIGTGSTASQLVPPIA
                     EQALQLHVFQRTANWVLPRRDRLYTALDRMLAHFPPYAAMVRWNWTQVLEWGRSGFDE
                     GTLARRALLAAAGALRKKQVSNEALRQKLVPPYPLGCKRIIYSNDFYPALMRPNVELV
                     TDPIARITPHGIVTTDGRERTIDALVCATGFDVAHLLSSIRIAGLQGRTLAETWAQGP
                     EAYHGITVSGFPNLFLMLGPNTATGHTSTLLYIEPEVDHAIACMQAVRGGNHRYIDVR
                     AEVQRKHNEHLQARLGTSVWSQCRSWYRMENGRVFALFPGFTREYVQSVRMPDLADYV
                     FSAPSSPAQPVTHAAASAQP"
     gene            11008..12213
                     /locus_tag="PKV56_08420"
     CDS             11008..12213
                     /locus_tag="PKV56_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX67881.1"
                     /translation="MNDYLLWGAGAVVVLLLVLLLVQRRRTPAPRREQPPSRPQSADS
                     ATRAADASPSRARGASQFDTVNAVHDMALAPREGVDVSLEADREAAEAAERAARAEAD
                     REAAEWEAQLVAQLLADQERERAAVEAERLAAEVAAAAPPEPDEQAIREQADREAAEW
                     EAKLVAQRLAEEESEREQARVESERLAAEAAAAERLQRDIAAHAALEQAAQQRFERAA
                     AEQQARLDAQRVAEEQAHAEAARLAEAAVAAAAAASAPSRSSAGAPEVPSAAAGPRTP
                     EQCLVMIADDSKVVRIKTSRLLAKHLYRVSLAEDGLDAARQIEIDLPDVLITDVEMPG
                     MDGFELTRQVRSNPRTAHIPIVMITADDEKHRADAAEAGVSVLLSKPYPEDQLLSYLQ
                     STLSGAAVH"
     gene            12284..13858
                     /locus_tag="PKV56_08425"
     CDS             12284..13858
                     /locus_tag="PKV56_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOX67882.1"
                     /translation="MDDKVIVSNRRALRAKYSTGGFAKVLAAIRSLIAADRQRGIRSR
                     LVFLDEAAAMKRLGATPVTSASSTRQAKAAIDAVFRATDPEYLMILGAPDVVPQQDLA
                     NPLFSPPDDPDASAWGDLPYACEARYSRDVSVFKGPTRVVGRLPDLAGATDPAYLLGV
                     LESAARYQSRDVADYAAYFGLSTRTWRESTALSLFNIFGNSKALTLCPPAAAAHSDAR
                     LAPLMHFINCHGGQADPQFYGEDAKHRFPVSLSSTGLAGKIRPGTVAAAECCYGGEMY
                     DSVTLGLPMPIGQHYLAQGACGFLGSSTIAYGPPDSNGAADLLAQYFLLAVLEGASTG
                     RAMLMARQQFVQQTAELDPADLKTLAQFSLLGDPAVHPARVPNATSLPRGLASSDALR
                     MQRRERRARLRAVGDFLEATKPATSKPQVDAACSAQVRRSLAGIAQSAGLPARQSFVS
                     YAVRAAGGSSPPPATGTRSPRGKPPAAATPVRYHVAIATPPRTVRAAAAPLSAVAAVA
                     REVDGRIVAYRVYEQR"
     gene            13863..14132
                     /locus_tag="PKV56_08430"
     CDS             13863..14132
                     /locus_tag="PKV56_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67883.1"
                     /translation="MNVSDAGPQRVDALEGRVVAGPVGAGSKSQREAVWIETVQGRYL
                     LRRRTGPSHGDATLARYVGMRVVCSGFIVGNSLLADRIDVIGPGV"
     gene            complement(14140..14721)
                     /locus_tag="PKV56_08435"
     CDS             complement(14140..14721)
                     /locus_tag="PKV56_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX67884.1"
                     /translation="MNATGTSPTLLVALLDRHSIGQRYLIEPGPSDDELRTLTQAAMR
                     APDHAALVPFRFRAVRAEARARMASLFEQAALAAGKDAESAALDRERASTAPVTVAVI
                     ARVDLGHPQVPAHEQWACIGGAIAHFLLAAQALGYGAKMLSGAKARSPLLVTAFCEPG
                     ETLVGWIALGTPQLDRRAKHIKPPADEVLTFWS"
     gene            complement(14726..15556)
                     /gene="phnE"
                     /locus_tag="PKV56_08440"
     CDS             complement(14726..15556)
                     /gene="phnE"
                     /locus_tag="PKV56_08440"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOX67885.1"
                     /translation="MSSPEVIDRILEAPPPRHALRWIGLGVAALVFLSFATLDLRWSD
                     FLSRGAMQSLGRFAASFFPPESSPGFLRRTLDGTLETLAMSLLGTLLAAMAGLALALG
                     ASARSHDGHRSIATLVLRGATRTALNVLRSIPELVWAALLLIAAGLGPFSGTLALALH
                     TSGVLGRLFAESMENAPAGPAFALRARGIGRLRVFLYATLPQVWPQVISYTLYRWENN
                     IRAAAVLGVVGAGGLGQMLYYHLGLFQMRESCTVLIAMVLLVALVDALSYAARRWLNR
                     "
     gene            complement(15553..16338)
                     /locus_tag="PKV56_08445"
     CDS             complement(15553..16338)
                     /locus_tag="PKV56_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX67886.1"
                     /translation="MRWATVLLLAVMLWPLAVLTEFRPWILFDERSLSSTLHFLATFV
                     PPRMDAEFLLLLARETWRTIAIATAGMTLAWLLAVPLALGSTARLSISALAQPMGLLP
                     FAVRTALRWTMVVLRSVPELVWALLFVRVVGLGPTAGVLAIGIAYGGMLGKVYAEILE
                     SGDPAPTQALLRNGSGRLQALLFGTLPQCGSELVSYTVYRWECALRASVVLGFVGAGG
                     LGQLLDSAAKMFSGGEVSTILLVFIALVALSDRISALLRRALA"
     gene            complement(16409..17281)
                     /locus_tag="PKV56_08450"
     CDS             complement(16409..17281)
                     /locus_tag="PKV56_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX67887.1"
                     /translation="MARTAAPGAVAEVVASLKDLHAAAAPLSRSEVLRGLSLEIRRGE
                     QLALIGSSGAGKTSLLQVLACAVAPLSGALSLFGSDPQALSSSERQRLRGRLFLAPQV
                     PPLPPRQRVVTAVLAGRLPAQSLLDSLLALWHPREAELARAALTALDLGDKLWDRVDR
                     LSGGERQRVGLARALVADAELWLVDEPLSALDPARAEQVIDRLTGAAREAGRTLVCTL
                     HQVDVARRSFARIVALRDGLIVYDGPAAAFTDAQVVALYGTRGLVGEADPPCIDSAAR
                     IAPGGTKPVQLICR"
     gene            complement(17281..18159)
                     /locus_tag="PKV56_08455"
     CDS             complement(17281..18159)
                     /locus_tag="PKV56_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX67888.1"
                     /translation="MLSTLHHAARWARSFGLATATAALALTAQAQQVLRVTAIPDEAP
                     TELARKFAPLGQYLEGKLGMKVEWTPVTDYAAAVEALVNKKVDLAWFGGFTFVQASVR
                     SDGKVIPLVQREEDEKFRSVFITDAKSGINKLEDLKGKTLSFGSASSTSGHLMPRSFL
                     LAAKINPDLDLKRISFSGAHDATIAAVASGKVDAGALNISVWDKFVADKKVDPAVVKV
                     FYTTPPYYDYNWTVHADMPADLRSKLQQAFLSLDASTPQGKEILGLQRATRFVSTKAE
                     NYTGIRAAAENAGLLK"
     gene            complement(18221..18649)
                     /locus_tag="PKV56_08460"
     CDS             complement(18221..18649)
                     /locus_tag="PKV56_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67889.1"
                     /translation="MRSILEDPPLEPTPTGRTPAGIALMLWTCDPTEPHRLATPFAQA
                     AAASALGLPVEIHFTARSVLLLQPGVAEILRPSPFDPKTVLDSMREAVDRGAVLVACT
                     DALQAHGMNPRSLIPECSRRGGPALFMGRLCDPDWRTLVF"
     gene            19207..20859
                     /locus_tag="PKV56_08465"
     CDS             19207..20859
                     /locus_tag="PKV56_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX67890.1"
                     /translation="MSAVAQPLPHSSSQTKHVAAGRTGFFAHHGIWAPGVRLFRMLRF
                     TSKAMIISLAFLLPLLGLVGWQLQAQADQAMQTRMDATRQHVEIAQGLLVWAHAQEID
                     GTLTREQAQRLAINAVSKLRYDGKEYFWINDMQPRMVMHPIKTELDGKDLSGVKDPNG
                     FALFNEFVSQVRKEGKGFVAYQWPKPGSDKPVDKISYVQGFEPWGWVIGSGVYTSDIH
                     ESMMRNLAWDGGVVAVALLFAGYLFLSFYRVMDGGLKETRRHLRAMTEGDLTTSPSPW
                     GNDEAAQLMLELRNMQESLRRMVSRVRASSDDIVSSSSDIASGAMDLSGRTEQAAANL
                     EESAASMEQIASTVKSTTEHTDEASKVARRNAEVAAEGGAVMRDVVETMDGIRASSAR
                     IGEIIGTIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIK
                     TLIGTSVEQVEAGTSIVRRAGVTIEEIVSSSGRVNQLLGEVATAAREQSQGIGQIGQA
                     VQELDRMTQQNASLVEHTAGAASAMRDQARTLADEVARFRMP"
     gene            complement(20872..21462)
                     /locus_tag="PKV56_08470"
     CDS             complement(20872..21462)
                     /locus_tag="PKV56_08470"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOX67891.1"
                     /translation="MPARRILLIQGHPDPAGGHLCHALAQAYSEGTAAAGHDVRCIEV
                     GQIDFPLLRTQAEWNDQPVPGSLLPSQQAIAWAQHLVIFFPLWMGDMPALLKGYFEQV
                     ARPGFAFDKSKGGPLGQKALGGRSARVVVTMGMPALVYRYYFRAHSVKSLERNILGFV
                     GIAPINETLIGSVDSLGPEGVAQWKSKLRTLGTQAQ"
     gene            21668..22063
                     /locus_tag="PKV56_08475"
     CDS             21668..22063
                     /locus_tag="PKV56_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOX67892.1"
                     /translation="MNSSITEDQRATLAEALKTRRLALERQMALHQHDQTRVEFARDL
                     LLQDGDDAPQRASDREVDQALSDIDLQALAAVSEAQARLQRGNYGQCVDCEEPIRFER
                     LLIEPEAERCLACQSTHETTHEISRQRTQ"
     gene            22065..22445
                     /locus_tag="PKV56_08480"
     CDS             22065..22445
                     /locus_tag="PKV56_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67893.1"
                     /translation="MSLFHAVVWTDHQSAQVLQFDAEHVKANKVKAHSHHTAQHGSQV
                     RTEHEFFGELCDSLDGITEVLATGSRTALADLRHYAEKHRPQIAARIVGYEVVDHPSE
                     KQLVALARKFFVEHDRMAGIPTPT"
     gene            22753..>23706
                     /locus_tag="PKV56_08485"
     CDS             22753..>23706
                     /locus_tag="PKV56_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX67894.1"
                     /translation="MHVPPALPSVPERPSLLVWLTAALVALMLLAGCSKAPPVAAPRG
                     PVEVGVHVVEAERLAINTELPGRTAAWLVAEIRPQVGGIVQRRLFDEGSRVRAGQALY
                     QIDPATFAAGQASARAGVAKAEATLHSAELTARRNAELAKIDAISQQSNEDSQAALQQ
                     ARADLGVAMAALDVARINLERTRITAPIAGRVDTSTVTAGALVTANQTQALTTVQQLE
                     PMYVDVTQSSAELLRLKRDLADGMLRRAAGGPAGATPIRLLLEDGSLYPHAGVLTVSG
                     VSVNAGTGSVTLRAVVPNPEGWLLPGMYVRAVLEQGLIEQAL"
BASE COUNT         3978 a         7867 c         8057 g         3804 t
ORIGIN      
        1 cccttaccat ccggcgcggt cacccaggaa aatagcccga tgaaaacacc acacacactc
       61 tctctccctc gctgcgcact cgcgttgttc ggtgcggcag cgctgttcgg agcatctgca
      121 caggccgctg acggcggtca ggcctacgtg tcgaatcagg ctggcaacgt ctcggtcatc
      181 gatctcgcga cgctcgaagt cacccgggag atcgatgcct acggcaagga gccgcgcggc
      241 ctcggcgtca ctgccgacgg caagctgctg atcactgcca accggggcga cgggaacatc
      301 tcggtgatcg accgcgccac aggcaatctg ctgaagaaca tcgcgatcgg caagaacccg
      361 gagttcgtgc ggacccgagg ccatttcgcg ttcgtttcct tcgaaccatc ctccaccggc
      421 ggtccccctc cgaagccggg ttcacccgag gcgatggcgg cagagaaggc caaggacgat
      481 gacgacgaga gtccggccta cgtggccgtg gtcgacctga aggcaggcaa ggtgatcagg
      541 cgcatccgcg gcggaatgga gaccgagggc atcgagttct cggccgatgg caagcacatc
      601 ctggtcacga acgaggcgga cgacaacgtc accgtccaca gcatcgccag cggcaagaag
      661 gtcaagacca tcgacgtgaa gtcctacggc gatcgtccgc gtggcatcaa gcgcgcaccc
      721 gacggcaagt cctatgtcgc gaccatcgag catggcaaca cgctggttgt gatggacgcg
      781 aaatacaacg tcgtcaagtc ggtgccgacg ggcgatacgc cctacggcct ggccttcgac
      841 cccaccggcg agcgcctgtt cgtcgccaac tcgaagagca agaacctgca ggtctacgac
      901 gcgaagacct acgagacgat caagaccatc ccgaccggtg accgctgctg gcacttcagc
      961 ttcacgcccg atggcagcca gatcctggtc gcctgcggcc gctcgaacga ggtggtggtg
     1021 atcgacgcga agacgctgga accggtcaag cgcatcgagg acaagaagct gccctggggc
     1081 ctggtcacct atccgaaggc attcggcagt ctggaccagc ccgagtgagt cagggtaacc
     1141 tcgggtaatt gctacccgag cgccccatga cggccactcg atccccccga catcctcgag
     1201 cccggccccc ggccgcagtg acggccgaca gcgaagaaac catcctggtc ctgcagggtg
     1261 gcggggcgct gggcgcttac cagggcggtg tgttcgaggc attgagtgcc gtccaccacg
     1321 agcccacctg ggtcgcaggc atttcgatcg gcgccatcaa cgcggcgctg atcgcgggca
     1381 acccgcctgc tgaccgcacg gcgcggctgc gcgaattctg ggacctggtc agctcggcct
     1441 tgccgacacc acctatcgtc gcgggcgcgc gtgcccgcga ggcgctcaac gagatcagcg
     1501 ccacgcaggt gatgcagttc ggggtgcctg gtttcttcca gccgcgcttt ccgccagcac
     1561 cgttccagcc gatcggcaca ctggaggcga tcagcttcta cgacaccacg ccactgaagc
     1621 agacgctgga gcggctgatc gacttcgacc gcatcaatgc gggcccgatg cggctgtcgg
     1681 tcggcgcggt caacgtgcgc accggcaact tcgcgtactt cgattccgcc aagcagcgaa
     1741 tcgacgtgcg tcacatcatg gccagcggcg ccttgcctcc gggctttgcg ccagtggaga
     1801 tcgacggcga gcatttctgg gacggcggtc tggtctcgaa cacgccgctg cagtacgtgc
     1861 tggaccagcc cggcaagcgc cgccgggtcg ttttccaggt cgatctgttc gcggcggtcg
     1921 gcgcactgcc caccacgctg cccgaggtga gcgagcgcga gaaggacatc cgcttctcca
     1981 gccgcacccg gctcaccacg accaacgagc tcgaccggca ggtgatcgcg caggccgcgc
     2041 aacggctgct cgcgaagctg ccacccgaat tgcgccacga ccccgatgtg ctcgccctgt
     2101 cgcgcgtgcg ctgcgaacac gcggtcgacg tggtgcacct gatctaccgc agcaagcact
     2161 acgagagcca gtcgaaggac tacgagttct ctcgcctgtc gatgcaggag cactgggctg
     2221 cgggccaggc cgacatggcc cacactctgc acgatccccg ctggctgcag cgcgagcgtc
     2281 acccgcacgg ggtgcgcgtg ttcgatctca ccggcgacag tccccacctt tacaactcgg
     2341 aaactgcctc atgaagcttc aggacaaggt ctgcatcatc accggcgctg ccagcggcat
     2401 cggcaaggaa atcgccatcg tctacgcccg cgaaggcggc aaggtcgtca tcgccgacat
     2461 gaacaaggtc gccgcccagg ccacggccga cgagatcacg aaggccggtg gcacagcgat
     2521 ggccgtggcg atggatgtga ccagcgagga gcaggtcaac gccgcggtcg ccgaggcggt
     2581 ggcggcctat ggcggcgtcg acgtgctcgt cagcaatgcc ggcgtgcaga tcgtccaccc
     2641 ggtcgaggac ttcccgttcg ccgactggaa gaagatgctg gcgatccatc ttgacggagc
     2701 cttcctcacc accaaggcct gtgtgccgca catgaaggcg tcgggcaagg gcggcagcat
     2761 catctacatg ggctcggttc actccaagga agcgtcggtg ctgaagtccg cctacgtgac
     2821 cgccaagcac ggcctgatgg gcctgtgcaa gaccgtgtcc aaggaaggcg gcaagcacgg
     2881 catccgcgcc aacgtgatct gccccggctt tgtgcgcacg ccgctggtcg acaagcagat
     2941 cccggagcag gcgaaggcgc tgggcatcag cgaagacgat gtggtcaaga aggtgatgct
     3001 caaggaaacc gtcgacggcg aattcaccac ggtgcaggac gtggccgacg tggcgttgct
     3061 gtttgccagc ttcaactcga acgcgctgac gggccaatcg ctggtggtca gtcacggctg
     3121 gttcatgcag tgacgattcg ccgctgaaag gccccggcct cgcccggcca cgacattcgt
     3181 tggtggcctc cggcggcttc ccgatgcggc gggctattcc ttgagtccgt gcttctccag
     3241 ccggtatctc agggtgtccc ggctgatccc gagggcgcgc gccgcctgcg acacgtttcg
     3301 attcgattcg gccatcgcct ggatgagcag ttcccgctcg cggcggtcga gcgccgagcc
     3361 cgcctcggtc gatggcgccg ccggggccgc gctgccggcc gctgacgtcg aagctgcgct
     3421 tgaaagcatc agctcgcgcg gctcgatggt gtcgctggcg gccagcatca ccgcctgctc
     3481 gatgacgttt cgcaattcgc gcacgttgcc gggccatcga taggcggcaa tcgccgcttc
     3541 ggccgacgcc ccgagcttga cgccctcgcg acggtatcgc tgccccatca gcttgcagaa
     3601 atggcgtgcc aggaagaccg catcgtcttc gcgatctctc agcggcggca ccatcagctg
     3661 cagcacgcgc aggcggaaat acaggtctgc gcggaacacc ccttcggcga cgcgcgcttc
     3721 gaggtcgtgg ttcgtggccg cgacgatgcg cacgcagacc tgatgctcct gcaccgagcc
     3781 gatgcgccgg aaacggccgt cctcgatgac cttgagcagc ttggcctgca gcgacaggtc
     3841 catgtctccc acctcgtcga gaaacagcgt cccgccgtcg gcggcttcga gcaggccgat
     3901 cttgcgttcc ttggcgtcgg tgaacgcgcc ccgctcgtgg ccgaagagct cggtctctag
     3961 cagggtgggc ggcatcgcgg cgcagttgat ctcgatgaac ggcttggcgc tgcgcgggct
     4021 caggtagtgg caggcccgcg cgaccagctc cttgccggtg ccggtttcgc caccgatcag
     4081 gatcggcggc agcatgccct cggcgtcgat cgacgccatc cgctcgatgc gctgctgcag
     4141 ttcccggatg gccggagact ggccgaggat cgactggctg gcgctgcgtt cacgcgcctc
     4201 gaaatagcgc agccggcttg catcgcgctg ctgctgcatc agcacgtcga tccgctcgcg
     4261 cagttccagc agcgacaccg gcttggtcag caggtcgttg gcgccggcct tcatcgcgtc
     4321 gacggccagc tcgatggtgc cgtgcccggt gatcaggatg atgtggacgg ccgggtcgct
     4381 ggcacggatg tgctcgatga actcgagccc ggtcccgtcc ggcagccggt agtcgagcac
     4441 cagggcctcc ggcttctgcg tctcggccag ggccttgcct tcggccacgg tggccgcggt
     4501 cgacaccgtg tgaccgaagc gctccaggta cttggcggca ttcctggcaa aagtgacttc
     4561 gtcgtcgacg atcaggatcg tggccatgga tcagattctc ggcaggtcga tgcaaacggt
     4621 cgtgccttcg cccgggcggc tgtcgatctg gagcacgccg cccaggcctt cgacgatgcg
     4681 cttggcaagt ggcaacccga gtcccagccc ctgcggcttg gtcgtgaaga acggcctgaa
     4741 gatgtgcgcg agcacctctg gcgtcatgcc ggggcccccg tccttgactt cgatgcgcac
     4801 ggtacggctg tccgtctcgc gaaccgtcac atcgcagcgc tcgcccgggg ggatggcctc
     4861 ggcggcgttc gagagcaggc tgtcgaacac ctgctgcagc aggtggctgt cggcctggac
     4921 cacggccttg gcgatgtggc tttccaggga cagggtcttg cggctgcgct ggaaggtgat
     4981 cttgtgatct gaaacgcagt tgcgaagcac gctgaccacg tcgaccggct gactcgcctg
     5041 cacgccctgg ttcgacagcc gcagcaattc ggtgatgcgt gccgagatgc ggtcgacctc
     5101 gcggatgatg tcgcgcgagc aatcggcact ctgctccgga ggcagctcca ggcccagttc
     5161 ggccgacgac ctgatcgaag ccagcggatt tcggatgttg tgtgccacgg acgaggccag
     5221 ttcgcccagc gcagcgacgg tgccggcctc cagcagcttg gtgtgctgct cgcgcatcgt
     5281 gcggtcggca cgccggatca gccagaacag gctcacgaac aacgccacgg cgccccccag
     5341 cgcggcgatg gcgacctgtc tctcgccctc ctcgatggcc tcggtgagtg ccagcggtgc
     5401 cttgtacaac tcgaccacgc cgacgacctg gttgtcgctc gggtcgacga cggggatgta
     5461 ggtttccacg aagaactgca ccgacgggga cagcccgatg tgttcccttt tggagagggc
     5521 ctcgtggctg atgcgtcccg actcgacctc gagcttgccc gccatggcgt gctcgaggtc
     5581 atcgttgtct gtgaagcggc gtcccaccat cgtcgggtcg ctcgaccaga cgacggtccg
     5641 atcgatgccg tacacattcg cgcgcagcat gtcccgcatg ttggcgaggt gcgtcacggt
     5701 gttcttgaag tgttcgacga cttgcgggtc gtccgtatgc cgcaggtagg cgagcgtgcc
     5761 gtcggacacc aggatgttct gcacgaaatc gcgcgagatc tcgccttcgc gcttgaacat
     5821 ctggctggtg aggaagcgcg acacgaccca ggcattggcc atcgcgatca gcacgatgac
     5881 gaccgcactc agtccggcga accagcgaag caactgaaac ggggcgggtt gggacggggg
     5941 catgttcggt ggcaaaaaga agactctggg gctgattctg gagtgcggtc ggcggtgatc
     6001 gtaagccgtt ggctgcctcg actcggcgag cggcgggcgc ccgcggatcg tggtcggggc
     6061 tgggatgcac gctttgatcg cagttgccgt gcccgcaacc gggtgctcga caaagcgttt
     6121 ccaatcaacc acttgggtct gccaagttgg ggtgttgact gcgaagtggg gcatatcgcg
     6181 cagttgaaat gggtgatatg ccccaacgtc cccggcgagc ccgggaccac tacttgagga
     6241 attcgcgcac cgacgtgatc gattcctgcg ccgcttcctc gtgcggcagg tgaccgaccc
     6301 cgggcagcga caccagcttg gcgccggtca tttccttcag gtagtcggcc gagttggcca
     6361 gcgggatcat cgcgtccgcc tcgccccaca gcagcagcgt cggggcccgg atgctgcgca
     6421 acagcgggcg cgggtcgacc agcacggact gctgcatccg tgcgatcagc gcctcgcggc
     6481 tgcccggtgc cagcatcagg tcgtggtagc gggccgtcag cgcatcgctc aggaacgcgg
     6541 ggtcgccgta ggccggtgcc aggctcatgc gcagcagcgg cttggggagc acgaagcgca
     6601 tcatccggac agcggccggt acctccggtg cttttccata ttcgaagccc gggctcgcga
     6661 aaccgtcggg tgcgaccagc accagcttgt cgacgcgctc cgggtgccgc gctgcaaagt
     6721 tccaggcaat gcgtccgccg atcgagtgtc cgaccacgct ggcacgtgcc acgcccagat
     6781 cgtccatcag cgcacgcagc aactgcaggc tgcgggcatc ggtgtagtcg ccggtcgggt
     6841 cgggcgaact gagcccgctg cccggcagat cgaagcggat cacgcggtga tcggctgcca
     6901 gcgtctgcgc ccagggttcc caggtgtgaa ggctgccgcc gaagccgtgg atcaggatca
     6961 cggctggcgc gtccttcggg ccgctgtcgc gcacgtgcag tcgaacgccg gcgatctcga
     7021 tgatgtcgct tggcgattgc aggtaccgcg cttccagatc ggcccggtcc ttgtccgggg
     7081 tccagagcca gaaggcgatc agggcaagca gcaacaagaa ggctccggtg gccaagctgg
     7141 ttttcaaccg cacgctttcg gcaaagagga atcggctgtg cactgtgcca tgaacgccta
     7201 gcgggccacg catcggctga gcccggggcg cacgaccgca cgccccgggc aagcttcaaa
     7261 gtgtcggcaa tagcaccttg ccaatgacat ggatgacgcc gttgctggcc cgcacgtcgg
     7321 tcgcgaggat cggcgccggc gtcgcggtct ggtcggtgat ggtcggcggg gtgccggcat
     7381 tgatcgtgat cggctgcccg gcgacggtgt tcaccggtgt gccgaacggg atgtcggcgg
     7441 ccagcacctg cgagccgagc acgtggtagg tcagcacggt ggtgagttgg ggcgtcgtca
     7501 gtcccgccac cgtcgatgcg atgtcggcga agactgcgtt ggttggcgcg aacaccgtga
     7561 acggaccggt gccgctcagg gtgccctgca ggtcagccgc gaccacagca ccgaccaggg
     7621 tgtcgaacaa ggggttgacc tgggccatct ggacgatgtt caacacgccc ggtggtacca
     7681 gcaccttgtc gatggcgtgg atgatgccgt tgctggcgtt gacattggcg gcggtcaccc
     7741 gggcggtcgt cagcgcagcg tcggtgatcg ccacgccgct ggtggtgttc agcgagagcg
     7801 tcgccgggct gcccccgaag ctgtagagcg tcccctgggt cgccccaccc gcggcaagat
     7861 cggcggcgct cttttcatcg ggcagcaagt ggtaggtcag gatgttggcg agcaccggcg
     7921 ccggcagcgc gtcgaccatc gccgcggcgc tggcaaagcc cagacgattc gccagttgcg
     7981 tgaaagccgc atcggtcggc gcgaacaccg tcaggctcgc gttctcgttc gtcaacgcgc
     8041 ccgctacccc ggccttgttg accgccgcca gcagtgcgtt gaagccctgt gcctgtgcca
     8101 ccacggcaat gttttcgggg tcgctgctgc ttccgccacc gccgcatgcg gccatcaggc
     8161 ccgccgtcgt gaaggcgagc atcgtcttga ggaaacggtt catcactggt gctcctgagt
     8221 cggatgaaat acgtccgtcg gggattcgac cggccgaccc aatgacccca gtcttaactg
     8281 attagttcaa ataaaaactg attcgtatgt gttctgacgt agcggtatta cttagggctg
     8341 tgcggacgcc gcggcgtggg tgacgggctg ggctgggctg gaaggcgcac tgaaaacgta
     8401 gtccgccagg tccggcatgc gcaccgactg cacgtactct cgggtaaacc cagggaaaag
     8461 agcgaacacg cggccgttct ccatccggta ccagctgcgg cactgactcc acaccgatgt
     8521 gcccagccgc gcctgcaggt gctcgttgtg cttgcgttgc acctctgctc gcacatcgat
     8581 atagcggtgg ttgcctccgc gcaccgcctg catgcaggca atcgcgtgat ctacctcggg
     8641 ctcgatgtag agcagggtcg aggtgtggcc ggtggcggtg ttggggccga gcatcaggaa
     8701 caggttcggg aaacccgaca ccgtgatgcc gtggtacgcc tcgggtccct gagcccaggt
     8761 ctcggccaga gtgcggccct gcaggcctgc gatacggatc gatgacagca gatgcgccac
     8821 gtcgaagccg gtggcgcaga ccagcgcatc gatggtgcgc tcgcggccgt cggtggtcac
     8881 gatgccatgc ggcgtgatgc gcgcgatcgg gtcggtgacc agttcgacgt tcggccgcat
     8941 caaagccggg tagaagtcgt tcgagtagat gatgcgcttg cagcccagag ggtagggcgg
     9001 cacgagcttc tgccgcaggg cttcgttgct cacctgtttc ttgcgcaggg ctcccgccgc
     9061 ggccagcagc gcgcggcgcg ccagcgttcc ttcgtcgaat ccgctgcgtc cccactcgag
     9121 cacctgggtc cagttccagc gaaccatcgc tgcatacgga gggaaatgcg ccagcatccg
     9181 atcgagtgcg gtgtacagac ggtcgcgtcg cggcagcacc cagttggccg tgcgctggaa
     9241 cacatgcagc tgaagcgcct gctcggcgat cggcggcacc agttgcgagg cggtcgaccc
     9301 ggtgccgatc accgccacgc gcttgccggg catcgcgtag ccgtgatccc agcgggccga
     9361 atgcagtcgc tcaccctgga aatcgtccag gccgggaatg tcgggccacc tcggctggct
     9421 cagcggccct gcgctgcaca tgaagaagcg cgactgcagc gtcgaacggg cgccttccgg
     9481 gcgctcgatg tcgtagtgcc agtggccggc cgcatcgtcg aaacgagcgg cgctgatgcg
     9541 tgttccgagg cggatgtgcg cgagcaggcc gaagcgcgtt gcgaccttct gcatgtaggt
     9601 ctggatttcg ggcgcaccgg cgaagcggcg cgtccagccc gggttgggcg cgaacgagaa
     9661 cgaatacacg ggcgacggca cgtcaacatg ggcgcccggg taggtgttgt cccaccaggt
     9721 gccgccgagg ccgtcctgct tttccaggat cacgaaatcg tggatgcctg cgcgcttgag
     9781 cttgaccgcg gcgcacagtc ccgacatgcc ggcgccgagg acgacgacct cgtggctcga
     9841 cgtggccggg ccgtttgcgg cggcagcctt cgccaggggg cgcatcacga agcgggcgac
     9901 ccgatcgatc gcctcgtcgg cgctgcgcag gacgccgccg ctggtctgga acacgtgcca
     9961 gcggtgcgcg gaaatctcgc agcgtgtgtc gaccccggct gcctgcaatg cggcctcgag
    10021 ctggagggcc tgtccgtgca gcagttcatc ggtgcccgcc tggatcagcg tcggcgggag
    10081 gccgcgcagg tcggcaaaca gcggtgacac ccgcggtaag tcgggggatg tgtcgcggag
    10141 gtagtgcgcg gcgcaggcgg cggcccaggc cacgctgagc atcgcctcgc cgcgaggctc
    10201 ggacgcgggc gcatcgcgca gggctacatc gacccacggc gagaacagca ccatcgccgc
    10261 gggcatcggc gagccgccct cgcgcagcgc cagcgcagcg gcgagcgcca gtccgccgcc
    10321 agccgaatcg ccggccagca cgacgggctg cccgcctcgg gccagtgcac gaaatgcggc
    10381 cacggcgtcg tcgagcccgg ctggaaaggg gtgctccggt gcgagtcggt agtcgagcga
    10441 aaaaaccggc agccccgtga ccagtgccag acgcgcggtc agcgatcggt gcgtggcggg
    10501 cgagccgacg cagtacgcgc cgccgtggag gtacagcacg acgccttgcg gtgcggacgg
    10561 ggcctgtgca gatcgcagcc attcgccggg cacgccaccg acctctgcgg tctcgatagc
    10621 cacgcccttc gggatcaggg tggtgcgcga cagcgcgagc agccagcggc gctggaagcc
    10681 aatcgaccag cgcggtgaaa acaccggctt cagcagcagc ttcagcgaag tccgcagcag
    10741 ggcggccttt aacccttcga cgatcccgct gggctggtag acgatcatca caggctcacg
    10801 gtgaaaagtg cggatcgtat ccggccgggt tttctgtggc atttccgcac gcgacttcgc
    10861 actgcagcgc aatttgatac atcgtgatac atcatttaac cgctgcgccg gtacgcttct
    10921 ttggccttgt tttcttgggt cttttcgtgc cgcagcggcg cgtgcgatcc gtggtgatgt
    10981 cagtgacgga tgggggtccg tgcgacgatg aatgactatc tcttgtgggg cgctggtgcc
    11041 gtggtggtgc tgctgctcgt cttgctgctg gtgcagcgcc gccgcacgcc tgcaccgcgt
    11101 cgggagcagc ctccgagccg tcctcagtct gccgactcgg ccacacgggc tgccgatgca
    11161 tcgccttcgc gagcgcgcgg agcctcccag ttcgacaccg tcaacgcggt ccacgacatg
    11221 gctctggcgc ccagagaggg cgtcgatgtc tcgctggagg cggaccgcga agccgcggaa
    11281 gcggccgagc gtgccgcgcg tgccgaggcg gaccgcgaag ctgcggaatg ggaagcgcaa
    11341 ctggttgccc agctgctggc cgatcaggag cgcgagcgcg ctgccgtcga agccgagcgc
    11401 ctggctgccg aggtcgctgc ggccgctccg cccgagcctg acgaacaggc cattcgggag
    11461 caggccgacc gcgaagctgc cgaatgggag gcgaagctgg tcgcgcagcg cctcgccgaa
    11521 gaggaaagcg agcgcgagca ggcgcgcgtt gagtccgagc gtctggccgc cgaggccgct
    11581 gcagccgaac gattgcagcg cgacatcgca gcccatgctg cgctcgagca ggcggcccag
    11641 cagcgtttcg agcgtgccgc cgccgagcag caggcgcgtc tggacgccca gcgcgtggca
    11701 gaagagcagg cgcacgccga ggcggcacgc ctggccgagg cagccgtggc cgcggctgcc
    11761 gcagcctccg cgccgtcgag gtcatccgca ggcgcgcccg aagtcccctc ggctgctgca
    11821 ggtccgcgca cgcccgagca atgcctggtg atgatcgccg acgactcgaa agtggttcga
    11881 atcaagacca gccgcctgct ggccaagcac ctctatcggg tctcgctggc cgaagacggg
    11941 ctcgacgccg cgcggcagat cgagatcgac ctgccggacg tgctgatcac cgatgtcgag
    12001 atgcccggca tggatggatt cgaactcacg cgccaggtgc gcagcaaccc ccgcaccgcg
    12061 cacatcccga tcgtgatgat caccgccgac gacgagaagc accgcgccga tgccgctgaa
    12121 gccggtgtca gcgtgctgct cagcaagccg taccccgagg accagttgct gtcgtacctg
    12181 cagtccaccc tgagcggcgc ggcagttcac tgaattcgcg gaaccggcga cccgggcgcc
    12241 gcaccgtcag caggccgggc gcgcacaggc ggagggagct cccatggacg acaaggtcat
    12301 cgtcagcaac cgacgtgcac tgagagccaa gtacagcacg ggtggtttcg ccaaggtgct
    12361 ggccgcgatc cgctcgctga tcgcggcgga caggcagcgc ggcatccgaa gccggctggt
    12421 ctttctcgac gaagcggcgg cgatgaagag actgggcgcc acgccggtca cctcggcgtc
    12481 gagcacgcgg caggcgaagg ccgcgatcga cgcggttttc cgtgccacgg accccgagta
    12541 cctgatgatc ctcggcgcgc ccgacgtggt gccgcagcag gatctggcca acccgctgtt
    12601 ctcgcctccg gacgatcccg atgcctcggc ctggggcgat ctgccctacg cctgcgaggc
    12661 acgctactcg cgcgacgtct cggtcttcaa gggcccgaca cgcgtcgtcg ggcgcttgcc
    12721 cgacctggcc ggcgccaccg atccggccta cctgctgggt gtgctcgaaa gcgccgctcg
    12781 ataccagtcg cgcgacgtgg ccgactacgc tgcgtacttc ggcctgtcca cgcgcacctg
    12841 gcgagaatct accgcgctca gcctcttcaa catcttcggc aactcgaagg ctctgacgct
    12901 gtgtcctccg gccgctgcgg ctcattcgga tgcacgactg gcgccgctga tgcatttcat
    12961 caactgccac ggcggccagg ccgatccgca gttctatggc gaggatgcca agcatcgctt
    13021 tccggtgtcg ctgtcgagca cggggctcgc cggcaagatc agacccggta ccgtcgcagc
    13081 cgccgaatgc tgctacggcg gcgagatgta cgactcggtc acgctgggtc tgccgatgcc
    13141 gatcggccag cattaccttg cgcagggtgc ctgcggcttc ctcggcagct cgaccatcgc
    13201 ctacggcccg cccgacagca acggtgcagc cgacctgctc gcgcagtact tcctgcttgc
    13261 cgtgctggag ggtgcctcga cgggtcgcgc catgctgatg gcacgccagc agttcgtgca
    13321 gcagacggcc gagctcgacc ccgccgacct gaagacgctg gcgcagttca gcctgctcgg
    13381 cgacccggcc gtgcatccgg cccgcgtgcc gaacgcgacc tcgttgccgc gtggcttggc
    13441 gtcgtccgac gcactgcgca tgcagcgccg cgagcgccgt gccaggctgc gcgccgtcgg
    13501 agactttctc gaagcgacca agccggccac gtcgaagcca caggtcgatg cagcctgctc
    13561 agcgcaggtg cgtcgctcgc tggcaggcat cgcgcagagt gccggcctgc ctgccaggca
    13621 gtcgttcgtg tcgtacgcgg tccgggccgc gggcggttcg tcgccgccac cggctaccgg
    13681 aacccgatcg ccgcgtggca agccgcctgc ggcagccaca ccggtgcggt atcacgtcgc
    13741 gatcgccaca ccgccccgca ctgtccgggc tgcagcagcc cccctctcgg cggtggcggc
    13801 tgtggcacgc gaggtcgatg gccgcatcgt cgcctaccgt gtctacgagc agaggtaagc
    13861 cgatgaatgt ttccgatgcc ggaccgcagc gtgtcgacgc gctggagggc cgcgtggtgg
    13921 ccggcccggt gggtgccggc agcaagagcc agcgcgaggc ggtgtggatc gagaccgtgc
    13981 agggtcgcta cctgctgcgt cgcaggaccg gccccagcca tggcgacgcg acgctggcac
    14041 gctatgtcgg aatgcgcgtg gtgtgctccg ggttcatcgt gggcaattcg ctgctggccg
    14101 atcgcatcga cgtgatcggg ccgggcgtct gaccggcttt caagaccaga acgtcagcac
    14161 ctcgtccgcc ggcggcttga tgtgcttggc acgccgatcg agctgcgggg tgccgagcgc
    14221 gatccagccg accagcgtct cgccgggctc gcagaaggcg gtcaccagca gcggtgaccg
    14281 cgccttcgcg ccgctgagca tcttggcgcc gtaacccagt gcctgggccg cgagcagaaa
    14341 gtgtgcgatg gcgccaccta tacaggccca ttgctcatgg gccggtactt gcggatggcc
    14401 gaggtcgaca cgcgcgatca ctgcgacggt gaccggcgcc gtcgaagccc gctcgcgatc
    14461 cagggcggcg ctttcggcat ccttgcctgc ggcgagcgcc gcctgctcga acagcgaggc
    14521 catgcgtgcg cgcgcctcgg cacgcacggc gcgaaagcgg aacggcacca gcgccgcatg
    14581 atccggcgca cgcatcgcgg cctgcgtcag cgtgcgcagt tcgtcgtcgc tcgggccggg
    14641 ctcgatcagg tagcgctggc cgatcgagtg gcgatccagc agggcgacca gcagggtggg
    14701 ggaggttccg gttgcgttca tgtgttcagc ggttcagcca tcgccttgca gcgtacgaca
    14761 gcgcgtcgac cagcgccacc agaagcacca tcgcgatcag caccgtgcag ctctcgcgca
    14821 tctggaacag cccgaggtgg taatacagca tctgtcccag gccgccggcg ccgaccacgc
    14881 ccagcaccgc ggctgcgcgg atgttgtttt cccagcggta cagcgtgtac gagatgacct
    14941 gcggccagac ctgcggcagc gtcgcgtaca ggaacacccg cagcctcccg atgccgcgcg
    15001 cccgcagcgc gaaagcgggc ccggcaggcg cgttctccat gctctcggca aacagccggc
    15061 cgagcacgcc gctcgtgtgc agcgccagcg ccagcgtgcc ggagaacgga cccagtcccg
    15121 ccgcaatcag cagcagtgcg gcccacacca gttcgggtat cgagcgcagc acgttcagcg
    15181 cggtgcgggt ggcgccgcgc agcaccagtg tggcgatcga acggtggcca tcgtggctgc
    15241 gagcactcgc gcccagggcc agcgccaggc cagccattgc ggcgagcagc gtgccgagca
    15301 gggacatggc cagcgtttcg agcgtgccgt ccagcgtgcg acgcaggaag cccggggacg
    15361 actcaggtgg gaagaagctc gccgcaaacc tgcccagcga ctgcatcgcg ccgcgcgaca
    15421 ggaaatcgga ccagcgcaga tcgagcgttg cgaaactcag gaataccagc gcggcgacgc
    15481 cgagtccgat ccagcgcagc gcgtggcgcg gcggcggtgc ctcgaggatt cgatcgatca
    15541 cttcggggct gctcatgcca gcgcccggcg cagcagtgca ctgatacggt ccgacagcgc
    15601 caccagcgcg atgaagacca gcaggatggt gctgacctcg ccgcccgaga acatcttggc
    15661 tgcgctgtcg agcagctggc cgagtccgcc cgcgccaacg aagcccagaa ccaccgatgc
    15721 gcgcagcgcg cactcccagc gatagacggt gtagctgacc agctcggatc cgcactgtgg
    15781 cagcgtgccg aacagcagtg cctgaaggcg tccgctgccg ttgcgcagca gcgcctgcgt
    15841 tggcgccggg tcgccgctct cgaggatctc ggcgtagacc ttgccgagca tgccgccata
    15901 ggcgatgccg atcgccagca cgccggccgt cggacccagg ccgaccacgc gcacgaacag
    15961 cagcgcccag accagttcgg ggaccgagcg cagcaccacc atcgtccagc gcaaggccgt
    16021 gcgcaccgca aacggcagca ggcccatcgg ttgggccagt gcgctgatcg acagccgtgc
    16081 ggtcgaaccc agcgcgagcg gcaccgcaag cagccaggcg agcgtcatgc cggccgtggc
    16141 gatcgcgatg gtgcgccagg tctcgcgcgc cagcagcagc aggaattcgg catccatgcg
    16201 cggcggcacg aaggtggcaa gaaagtgcag cgtcgagctc aggctgcgtt catcgaacag
    16261 tatccagggc ctgaactcgg tcagcaccgc gagcggccac agcatcacgg ccagcaagag
    16321 cacggtggcc cagcgcatcg gcagcgccgg atcgctgccg gagctgcgct gcgccgtcgt
    16381 cgatgccgtc gtcattcgtg ggtcgacttc agcggcagat cagctgcacg ggcttcgtgc
    16441 cgcccggcgc gatgcgagct gcggaatcga tgcacggcgg gtctgcctcg ccgacgagtc
    16501 cgcgcgttcc atacagtgcc acgacctgtg catcggtgaa ggccgccgcc ggcccgtcgt
    16561 agacgatcag tccgtctcgc agggccacga tgcgtgcaaa gctgcgccgc gccacatcga
    16621 cctggtgcag tgtgcagacg agcgtgcggc cggcctcgcg ggccgcgccg gtgaggcgat
    16681 cgatcacctg ttcggcacgt gccgggtcga gcgccgacag cggttcatcg acgagccaca
    16741 gctcggcgtc ggccaccagc gcgcgcgcca gcccgacgcg ctggcgctcg cccccggaca
    16801 ggcggtcgac ccggtcccag agtttgtcgc ccagatcgag cgcagtcagc gcagcccgcg
    16861 ccaactcggc ttcgcgcggg tgccacagcg cgagcaggct gtcgagcagg gactgcgccg
    16921 gcagccggcc cgccaggacc gcggtgacca cgcgctggcg cggcggcagc ggcggcacct
    16981 gtggcgccag gaacagccgc ccgcgcaagc gctgtcgttc actcgacgac agcgcttgcg
    17041 ggtccgagcc gaacaggctg agcgcgccgg agagcggcgc cacggcacag gccagcacct
    17101 gcagcaggct ggtcttgccc gcgcccgacg agccgatcaa cgcgagctgt tcgccgcgcc
    17161 gaatctccag cgagagaccg cgcagcacct cggaacggga cagcggtgct gccgctgcgt
    17221 gcaggtcctt cagcgaggcg accacctcgg cgaccgcgcc gggcgcggcc gttcgggcca
    17281 tcacttcaac agaccggcgt tctcggcggc cgcacgaatg ccggtgtagt tctcagcctt
    17341 cgtcgagaca aagcgcgtcg cgcgctgcag gccgaggatc tccttgccct gtggcgtact
    17401 ggcgtcgagc gacaggaacg cctgttgcaa cttgctgcgc aggtcggcgg gcatgtcagc
    17461 gtgcaccgtc cagttgtagt cgtagtacgg gggcgtcgtg tagaacacct tgaccaccgc
    17521 ggggtcgacc ttcttgtcgg cgacaaactt gtcccacacc gagatgttca gtgcgccggc
    17581 atcgaccttg ccgctggcaa cggcggcgat cgtcgcgtcg tgtgcgcccg agaaactgat
    17641 gcgcttcagg tctaggtcgg ggttgatctt ggccgcaagc aggaagctgc gcggcatcag
    17701 gtggcccgaa gtgctcgacg ccgagccgaa gctcagcgtc ttgcctttca ggtcctcgag
    17761 cttgttgatg ccgctcttgg catcggtgat gaagaccgaa cgaaatttct cgtcttcctc
    17821 gcgctgcacc agcggaatca ccttgccgtc cgaacgcacg ctcgcctgca cgaaggtgaa
    17881 cccgccgaac caggccaggt cgaccttctt gttgaccagc gcttcgaccg cggcggcgta
    17941 gtcggtcaca ggcgtccact cgaccttcat gccgagcttg ccttccaggt actgacccag
    18001 cggggcgaac ttgcgcgcca gttcggtcgg tgcctcgtcc gggatggcgg tgacgcgcag
    18061 cacctgctgt gcttgcgcgg tcaacgcgag ggcggcggtg gctgtggcca gaccgaatga
    18121 gcgcgcccag cgggccgcgt ggtggagggt ggaaagcatg gtcaatctcc tggagtgggg
    18181 cgcgggtgca cccgtggaac cagcccaatc tggctggagg tcaaaaaacg agtgtgcgcc
    18241 agtccggatc gcagagccgc cccatgaaca gggcggggcc gccgcggcgg ctgcattcgg
    18301 ggatcaggct gcgcggattc atgccgtggg cctgcagcgc gtcggtgcag gccaccagca
    18361 ccgcaccccg gtcgaccgcc tcgcgcatcg aatcgagaac ggtcttcgga tcaaacgggc
    18421 tcgggcgcag gatttccgcc acgccgggtt gcagcagcag gacgctgcgg gccgtgaagt
    18481 ggatttccac cgggagcccc agggccgagg ccgccgccgc ctgggcaaat ggagtcgcca
    18541 gccgatgcgg ctcggtcggg tcgcaggtcc acagcatcaa ggcgataccc gccggtgtcc
    18601 ggccggtagg ggtcggctcg agcggggggt cttcaagaat ggatcgcatg gctgattatt
    18661 caaccacgcc gatcgagtcc ttgctggcgg cgccatccga gggcacccgg ggccgcctgc
    18721 gccgtgagcg ctggcgtcag tcggtcaaac gggactgtcg aagccgccgc tcaggcggac
    18781 acccaggcgc gttcgtcttc gggagtgcaa cagccgtgat cccggtcttc gcgtcggttg
    18841 tttgcgccac tgcgggccga gcccgggtcg gcacgcccgg cgagtcgtcg cggacccggc
    18901 tggacggggc tccaacggtc cgttccagtc gagaaatgtc tgggccggac tgaacggctt
    18961 gcgaagtcgg aggcctcgac ggggtcgttg aggtcgattt gttgattcat gcccatcaca
    19021 acggagcttt cacgcgggaa gtggacagct cgatcgggtc acttgtaact taatgtgtat
    19081 cggcacctcc cgggtcgatc gcaactcaag cggccggcag taattcccga taagtacggc
    19141 agagaacggc ggcggttcgc tgctgccatt gccgcacata acaagaccag gcgaggaacc
    19201 cattcgatgt cagccgttgc ccaaccgctt ccccattctt cttcgcagac caagcacgtg
    19261 gctgctggcc ggaccggctt cttcgcgcat cacggcatct gggcgccggg cgtgcggctg
    19321 ttccgcatgc tgcggttcac ttcgaaggcc atgatcatct ccctggcgtt cctgctgcca
    19381 ctgctcgggc tcgtgggctg gcagctccag gcccaagcgg accaggccat gcagacacgc
    19441 atggatgcaa cgcgtcagca cgtcgagatc gcgcaagggc tcctcgtgtg ggcgcatgcg
    19501 caggagatcg atggcacgct cacgcgcgaa caggcgcaac gtctcgcgat caatgcggtg
    19561 tcgaagctgc gttacgacgg caaggagtac ttctggatca acgacatgca gccacgcatg
    19621 gtcatgcatc cgatcaagac cgagctcgac ggcaaggacc tgagcggggt caaggatccg
    19681 aacggcttcg cgttgttcaa cgaatttgtc tcgcaggtgc gcaaggaagg caagggcttc
    19741 gtcgcgtacc agtggccgaa gcccggcagc gacaagccgg tcgacaagat ctcgtacgtc
    19801 caggggttcg agccttgggg ctgggtcatc gggtcgggcg tctacacgag cgacatccac
    19861 gaatcgatga tgcgcaatct ggcctgggac ggcggtgtcg tcgcagttgc gctgctgttt
    19921 gccggctatc ttttcctgag cttctatcgc gtgatggatg ggggcttgaa ggagacccgc
    19981 cgtcacctgc gtgccatgac cgagggcgac ctgacgacgt caccgtcgcc ctggggcaac
    20041 gacgaagcgg cacagttgat gctcgaactt cgcaacatgc aggaatctct gcgtcgcatg
    20101 gtgagtcgtg ttcgagcctc cagcgatgac atcgtcagtt ccagcagcga catcgccagt
    20161 ggcgcgatgg acctgtccgg gcgcacggag caggccgcag ccaatctcga ggagtcggcg
    20221 gcgtcgatgg agcagatcgc ttccaccgtg aagagcacga ccgagcacac ggacgaagcc
    20281 tcgaaggtgg cgcgtcgcaa cgccgaggtg gccgctgagg gcggtgccgt gatgcgtgac
    20341 gtcgtcgaga cgatggatgg catccgcgcg tcgtcggccc ggatcggaga gatcatcggg
    20401 acgatcgacg gcatcgcatt ccagaccaac atcctggcgc tcaacgccgc tgtcgaggcg
    20461 gcacgcgccg gcgaacaggg ccgcggcttc gcagtcgtgg cgaccgaggt ccgttcgctc
    20521 gcgcagcgca gcgccgcggc agcgaaggag atcaagacct tgatcggcac cagcgtggag
    20581 caggtcgagg caggcaccag catcgttcgt cgggcgggtg tcaccatcga agagatcgtg
    20641 tcgtcgtcag ggcgtgtcaa ccagctgctc ggcgaggtgg ccaccgcggc acgcgagcag
    20701 agtcaaggca tcgggcagat cggccaggcc gtccaggagc ttgaccgcat gacgcagcag
    20761 aacgcctcgc tggtcgagca caccgccggt gcggcatcgg ccatgcggga tcaggcgcgt
    20821 accctcgccg acgaggtggc gcgcttcagg atgccctgat cagccagagc gctactgggc
    20881 ctgcgtgccc agggtgcgca acttcgattt ccattgcgct acaccctccg gtcccaggct
    20941 gtcgaccgac ccgatcagcg tttcgttgat tggtgcgatg ccgacgaatc cgaggatgtt
    21001 gcgctccagc gatttgacgc tgtgtgcccg gaagtagtag cggtagacga gtgccggcat
    21061 gcccatggtg acgacgaccc gggccgagcg gccgccgagc gccttctggc ccagtggccc
    21121 gcccttgctc ttgtcgaagg cgaagccggg gcgcgcgacc tgctcgaaat agcctttgag
    21181 caaggccggc atgtcgccca tccacagcgg aaagaagatc accagatgct gcgcccacgc
    21241 gatggcctgc tgggagggaa gcagcgagcc gggcacgggc tggtcgttcc actcggcctg
    21301 cgtgcgcagc aacgggaagt cgatctgccc gacctcgatg caccgcacgt cgtgaccggc
    21361 tgcagccgtt ccctctgagt aagcctgtgc cagcgcgtgg cacaggtgcc cgccggccgg
    21421 atcgggatgt ccctgaatca acaggattcg gcgagcaggc atggcggctc caggtcaagg
    21481 aggttcatgg tcacacgcgc cggcgcgcac gaattgatca aacgcaagcg cgcctgtcgt
    21541 ggcccgtccc ggctgtccct gccccgagtt cgtctgcagc tttgttgatc tgcatcacaa
    21601 ccgctgaaca acgatctgcc gatcatcgtc tggagtcccc atggattcag accccttgga
    21661 aaattgcatg aacagttcga tcaccgaaga tcagcgggcc acgctcgccg aggccttgaa
    21721 gacgcgcaga ctggcgctgg aacggcagat ggccctgcac cagcacgacc agacgcgtgt
    21781 cgagttcgcc cgcgacttgc tgttgcagga cggcgatgac gctccgcagc gtgccagcga
    21841 ccgcgaggtc gatcaggcgt tgtccgatat cgacttgcag gcgctggccg ccgtgtccga
    21901 agcgcaggcc cgcctgcagc gaggcaacta cggccagtgc gtcgactgcg aggagccgat
    21961 tcgtttcgag cgtctgctca tcgaacccga agccgagcgc tgcctggctt gccaatccac
    22021 ccacgaaacc acacatgaaa tcagccgtca aaggacccag tgacatgagc ctcttccacg
    22081 ccgtcgtatg gactgatcac cagtccgccc aggttctgca attcgatgcg gagcacgtca
    22141 aggcgaacaa ggtgaaagcc cattcgcacc acacagcgca gcacggcagc caggtgcgta
    22201 ccgagcacga gttcttcggt gagttgtgcg actcgctcga cggcatcacc gaggtgctgg
    22261 cgaccggttc gcgcaccgcg ctcgccgatc tgcgccacta tgccgagaag caccgcccgc
    22321 agattgccgc gcgcatcgtc ggctacgagg tggtcgatca tccgagcgag aagcagctgg
    22381 tggcgctggc gcgcaagttc ttcgtggagc acgaccgcat ggcgggcatc ccgacgccga
    22441 cctgagcgtc aacgcggctc ggacgcagcc cgaccccgct cgggcttgcg acgcagtctg
    22501 ccgccatgtc gatcaggccg tggtcggcgt cgcggccttc agctgttggc tgaagagccc
    22561 ggactgccgt tcgcagtcgc gggcattggc gaggttcctg agcaccgagt gcgcccgatg
    22621 agtgcgtgct cgagaggtgc cgctctcgac cggcgcaagc tccgaagacg gccgctgcac
    22681 ggccccggag acgtcctgcg agcccgggct tagatttcag ggcatcctcg ccctgttttg
    22741 tcgccctgtc gcatgcacgt tcctcctgct cttccgtccg tcccggaacg cccgtccctg
    22801 ctcgtctggc tgacggccgc gctggttgcg ctcatgctgc tggccgggtg ctcgaaggcg
    22861 ccacccgtgg ctgcgccgcg cgggccggtc gaggtcggtg tccatgtcgt cgaagccgag
    22921 cgtctggcca tcaacacgga actgccaggt cgcaccgctg cctggctggt cgccgagatc
    22981 cgtccccagg tcgggggcat cgtgcagcgg cggttgttcg acgagggatc ccgggtgcgt
    23041 gccgggcagg cgctgtatca gattgatcca gccaccttcg cggccggcca ggccagtgcc
    23101 cgggcgggcg tcgccaaggc cgaggccacg ctgcattcgg cggaattgac cgcgcgccgc
    23161 aacgccgagc tcgcgaagat cgacgcgatc agccagcagt ccaacgagga cagccaggcg
    23221 gcgttgcagc aggcgcgcgc cgatctgggc gtggcgatgg ccgcgctcga tgtggcgcgc
    23281 atcaacctcg aacgcacgcg catcacggcg ccgatcgccg gccgcgtcga cacgtcgaca
    23341 gtgacggcag gcgcgttggt cactgccaac cagacgcagg cgctgacgac ggtgcagcag
    23401 ctcgaaccga tgtacgtcga cgtgacgcag tcgagtgcgg agctgctgcg tctgaagcgt
    23461 gacctggcag acggcatgtt gcggcgggcc gcgggaggcc cggctggcgc cacccccatc
    23521 aggctgctgc tcgaggacgg cagcctgtac ccgcatgcgg gcgttctgac ggtcagcggt
    23581 gtcagcgtga atgcggggac cggatcggtc acgctgcgcg cggtcgtgcc caatcccgaa
    23641 ggatggctgc tgcccggcat gtacgtgcgc gcggtgctcg agcagggtct catcgagcag
    23701 gcgctg
//
LOCUS       DAOORV010000049        23644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2611_length_23644_cov_7.613252, whole genome shotgun sequence.
ACCESSION   DAOORV010000049 DAOORV010000000
VERSION     DAOORV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2611_length_23644_cov_7.613252"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PKV56_08490"
     CDS             complement(<1..1583)
                     /locus_tag="PKV56_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOX67895.1"
                     /translation="MTAKKSAPAAATKPATPVKGAASKSSGAKEELAAKTRASATAVK
                     SAAGAKPAAKATAKAGAGDDKPKRGRKPAKGDDKSKKAELGDEDFSDLEADLEGEPEA
                     EVAESADDGDSKVKVKPLRMKVSRAKERALMREFGLDETALTEEEVAKRRQELKTLIK
                     MGKTRGFLTHQEINDHLPEKLVDNEILEAIVSMLNDMGIAVYEQAPDAATLLIAGGGT
                     TTATEEEAEEAAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGG
                     LQAMMYAISASPTTIAEILVYADRITAAEMTISEVVDGFVSEGEADDYVAEEDVDFFD
                     EDDDDDGNGGSKALTKKLEELKTQALAKFENLRINFDKMRKAFEKEGYQSPAYSKAQQ
                     AISSELMTIRFTVKTIEKLCGILRSQVDDVRRYERELRKILVDKCGMPQDYFIKHFPP
                     NILNLKWAEKEVAANKPYSVVLARNLPPVQELQKKLTDQQARAVVPLDDLKKINKRMN
                     EGEKASRDAKKEMIEANLRL"
     gene            complement(1930..3771)
                     /gene="dnaG"
                     /locus_tag="PKV56_08495"
     CDS             complement(1930..3771)
                     /gene="dnaG"
                     /locus_tag="PKV56_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOX67896.1"
                     /translation="MISPSFIQDLLGRVDIADVVGRYVKLKKSGANHMGLCPFHGEKS
                     PSFSVSPSRQTYHCFGCGVHGNAIGFLMEHSGMGFVDAVQDLAQQVGLAVPQDDRSPE
                     ERERAQKTKERQGTLNDVLAKAADHYRKQLKSCPRAIDYLKQRGLSGEVAARFAMGYA
                     PEGWRTLAGAFPRYDDPLLEESGLVIAQDAEATPGADSKRYDRFRDRIMFPIRSVQGE
                     VIGFGGRVLDRGEPKYLNSPETPVFSKGRELYGLYEARTAIRQHGFVLVVEGYMDVVA
                     LAQLGFPNAVATLGTACTAEHVQKLFRFTDSVVFSFDGDAAGRRAAGRALEAALPHAT
                     DLRSVRFLFLPAEHDPDSYVREHGTEAFAACIGQAVPLSRQVVAMAQEGCDLATAEGR
                     ARFLAMARPLWTALPDGALKRQLLGDLAVVAKLDPAELSVMWQASPPTLRQPARNETP
                     PRPRLVRAARQGAANQLDRALWLLLHRAEMWWDLDGHCHDLLADRVAPYAALFGSFER
                     CLHDHGPLAPAPLLAELTQSVHEDEHARAALERIALFHEPEPHTDLRQQLDLILDHLR
                     LRDVDAELEQLFESGLMSPDIQARSAELMQSRARLKATLSGLASSPA"
     gene            complement(3894..4202)
                     /locus_tag="PKV56_08500"
     CDS             complement(3894..4202)
                     /locus_tag="PKV56_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOX67897.1"
                     /translation="MSTLKELLDRKAALDKEIESTRKQEHANAIEKVRALMQEYGLTI
                     SDISNKAPVRAASSTKGGKVAAKYRNKATGETWSGRGLQPKWLKAALSAGHQLSEFTV
                     "
     gene            complement(4373..4645)
                     /locus_tag="PKV56_08505"
     CDS             complement(4373..4645)
                     /locus_tag="PKV56_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOX67898.1"
                     /translation="MARIVTCIHLKKEGEGLDYAPYPGDLGKRIYNEVSKEAWQLWLK
                     HQTMLVNENRLNLADLRARQYLARQMENFFFGTGAEQPAGYVPPSA"
     gene            complement(4708..6048)
                     /gene="argA"
                     /locus_tag="PKV56_08510"
     CDS             complement(4708..6048)
                     /gene="argA"
                     /locus_tag="PKV56_08510"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOX67899.1"
                     /translation="MSLVFPHTFVPWFRSVAPYIHAHRSKTFVVAIAGELIAAGKLSA
                     FAQDLALIHAMGIKVVLVHGFRPQVEEQLKAKGHVSKYSHGMRVTDAVALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGSQIRVVSGNFITAKPVGVIDGVDFIHTGLVRRIDAVG
                     IRQAIDFGALVMLSPFGFSPTGEAFNLSMEDVAASTAISLQADKLIYLTEVRGIPLDI
                     SDPNSAIDQELALADAEKLLASLPNPQQPTDTAFYLQHAVKASKSGVDRVHIVPFSVD
                     GSVLIESFTHDGVGTMIVDEKLESLREATADDIGGVLQLIEPFENDGTLVKRDRTEIE
                     RDIDFYTVIEHDGVIFGCAALYPYPEKQTGEMAALTVSPDVQGQGDGERILKRIEQRA
                     RALGLKSIFVLTTRTMHWFIKRGFEQVGPEWLPEARQRKYNWDRRSQVLVKTLS"
     gene            6091..10017
                     /gene="hrpA"
                     /locus_tag="PKV56_08515"
     CDS             6091..10017
                     /gene="hrpA"
                     /locus_tag="PKV56_08515"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOX67900.1"
                     /translation="MTSAPRVRSTPRPNALPANPIPPITFADSLPVSARRDEIATAIR
                     DHQVVIVCGETGSGKTTQLPKIALTLGRGRGAGGSGLIGHTQPRRIAASSVAKRIAQE
                     LNSPLGEVVGYKVRFQDRLSPGATVKLMTDGILLAETQTDPLLRAYDTLIIDEAHERS
                     LNIDFLLGYLRQLLPRRPDLKVIVTSATIDAERFAQHFAGRNGPAPVIQVSGRLFPVE
                     QRWRPFEDSREYGLNHAIADAVDELWASGGAGSGGDVLVFLPGEREIRDAADHLRRHH
                     PPGVEVVPLFARLSQAEQDRIFEPHSARRIVLSTNVAETSLTVPGIQYVIDAGTARVK
                     RYSYRNKVEQLLIEPTSQAAANQRAGRCGRVSNGICIRLYDEKDFAGRPRFTDPEIMR
                     SSLAGVILRMKALGLGLVEDFPFIEPPPRRAIADGYQLLAELGAVDDQNELTPMGQEL
                     ARLPLDPRVGRMILEARNRQALTEVLIVASAMSVQDVRDRPLEAQQAADAAHKKFDDE
                     RSEFLGDLKLWKWLEASRGAGLTAEQREHKLSARKQEQLLRENFISPRRVREWRDIHS
                     QLLTVVAEHGWRLNASPATYEQLHLSLLAGLLGNIGVKSDEEDWYLGARGIRFHRHPG
                     INLSKKPGRWIVAAELVETTRLYGRGIAAIEPQWLPQIAGHVIKTQLLEPHWEKKAAE
                     VIALERATLYGIVVYNNRRVNFGLVNPAEAREIFIREALVAGDWETRLPFVAANRKLI
                     AQVEELEHKSRRQDVLVDDELIHAFYDQQLPAEVCSGATLERWYRDEIRRQPKLLMLT
                     RDELMRHEAAGITTAAFPKTLRMGGVDCGAIYLHEPGDAKDGVTVTVPIYALNQVSEE
                     RCEWLVPGMLKDKVLALAKSLHQRPRSRLVPLPEYAAAFISSVEAAHGWGSGSLVDAL
                     LKHVRDRTQLDVKRADFKLEQVPPHLHMNFRVVDENGRQLGMGRNLSTLKAELGSQAR
                     SAFQALAQLKSVQPERPIAADTASSVSRKPGAQRVAEAPAVSPAGRASVPPDSAAEHY
                     TDWTFGELPELLEVRRGDQTMVGFPALIDRGGAVVIEVFDEPEAAAARHREGLRRLVS
                     LQIRDALKYLEKNIPDLQKMSVLYMPLGTADELREQVISLAIDRAFLVDPLPTDAIAF
                     RRRVEEGRGRLTLIANEIARSAQAVLVDHAAALRKLRDARAPKEVSEDIEAQLSRLVP
                     KRFLGATPWSQLSHLPRYLRGIVMRLDKWRADPGRDASRMAELRPLEQRYLRTVIERK
                     GVRDTRLDEFRWLLEELRISLFAQELRTPQPVSVKRLEKSWLQMTQ"
     gene            10105..10181
                     /locus_tag="PKV56_08520"
     tRNA            10105..10181
                     /locus_tag="PKV56_08520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10139..10141,aa:Pro,seq:tgg)
     gene            10286..10359
                     /locus_tag="PKV56_08525"
     tRNA            10286..10359
                     /locus_tag="PKV56_08525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10320..10322,aa:Arg,seq:tct)
     gene            10484..11242
                     /locus_tag="PKV56_08530"
     CDS             10484..11242
                     /locus_tag="PKV56_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67901.1"
                     /translation="MFGALKRLLALGPGRAGGARFDAIDSWADRSGFRFRRAKDGLRF
                     ELEAASGGHVVRMEWGPSQRSYIEHSELRMRVDMNLPGSLQMLVLTLTLKERLESETF
                     ERYTQDAQTVIDMSTPEEMRWLAMFPKADLSFDTVLRERFGALGANPAWALAWINGPL
                     GAQLARAVPDLIGPDVPFVLMTMRGRIYLRMQLADPAPDALSQCLEVFDSAMRSVQQI
                     TGQISPDDAEWASTASTSWQTQLPDDEATDPTRF"
     gene            11314..13269
                     /locus_tag="PKV56_08535"
     CDS             11314..13269
                     /locus_tag="PKV56_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67902.1"
                     /translation="MLKAITTVAAGLLLALSSQPLMAVGFGRLVNATSLGQALDVSVA
                     LDADITEQVTSDCVSAEVVVGDSVLPRPVVRVRLEPAGNASTRSLRVTTTVRIHEPIV
                     QVTVAAACPSRVSRQFVVLIDPPLTASEPALAAAEPPAVATPPASPATSVSVGALPDP
                     GFAQGISDAAAGSGVLAPPPAPAPPASARRATRSTSKGVASGAKAARRAQAATPRKAR
                     RASVNQARARSSPRLELERGAGPLAAAAASAPLAVAGVLPVALAASAAASSPAESVAE
                     LQREAIQSLQQRLDKLQADSEASSRSIADLQARLRAAESSRSTGWLSYALAAAGAALL
                     LWFGQMIGRRAAARRPGTPWWKAEADAAAAKPVDAEDRASARPAFVQSALASLPEAIE
                     LPDEVLPTTPAPLVVPDHSRLPEPLRPVQVFGDTVASVGTALAVEPPVAPASALTADE
                     LIDLEQQAEFFVALGQDDSAVDLLNAFLRGGGGASPLPYLKLLEIHRRREERDAYERI
                     RDRHARRFGTEAPKWEDQTAAAVLADGQSPVMRQIESVWADPPEAMKLIESLLVSSDP
                     AAAPLDLAALTDLQFLYLLARAVREFEDQPAQPSAESTVDLLLPLSHQDEHRSVVASV
                     DSGFSTPSDIDLELDFLPPRNDPKPLA"
     gene            complement(13287..14468)
                     /locus_tag="PKV56_08540"
     CDS             complement(13287..14468)
                     /locus_tag="PKV56_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX67903.1"
                     /translation="MSLGTHAKTAYRTPPVTSRLPQVGTTIFTVMSALAQQHGAVNLG
                     QGFPDFECDPRLLDAVNHAMRSGHNQYPPMPGVPALREAIASKIESLYGRRYDPASEI
                     TVTAGATQAILTIMLAVVHPGDEVILLDPCFDSYEPNVALAGGVAVHVPLTPGSFRPD
                     FDRIAQALTPRTRLIVVNTPHNPSATVWTAAEMQQLTDLLRPTDVLVIGDEVYEHMVY
                     DGIRHESIARWPELAARSFVVSSFGKTYHVTGWKVGYVAAPAALTTEFRKVHQFNVFT
                     VNTPVQHGLAAYMRDPGPHLGLAMFYQRKRDLFADGLRATRLRPLPSAGTYFQCADYS
                     SISDEPERAFCERLTRETGVAAIPMSAFYADALEQRLIRFCFAKQTTTLELALERLRR
                     L"
     gene            complement(14465..15403)
                     /locus_tag="PKV56_08545"
     CDS             complement(14465..15403)
                     /locus_tag="PKV56_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HOX67904.1"
                     /translation="MNLHQFRFVQEAVRQNLNLTETAKALFTSQPGVSKAILELEGEL
                     GVDIFARHGKRLRRVTEPGQEVLKAIEIIMREVNNLKRIGDEFSNQDAGTLSIATTHT
                     QARYVLPEPVARLRKRFPKVNVSLHQGNPQQVARMVIEEVAEVGLATESLDRYDELVT
                     LPCYEWQHVIVMPAGHPLAQVERLTLEQLAVEPLISYHPTFTGRTRIDQAFESRRLRP
                     NVVLEAIDSDVIKTYVRLGLGIGIVAEMAVRDDPPGGDLVWRPAGQLFGQNVARIAFK
                     RGAYLRNFVYVFAEMISERLSRALITRSINGVPSTE"
     gene            complement(15400..15807)
                     /locus_tag="PKV56_08550"
     CDS             complement(15400..15807)
                     /locus_tag="PKV56_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOX67905.1"
                     /translation="MKTGLLLFAHGARDPNWSLPFRAVLESVRNRAPDVCVELCFLEF
                     MSPNLLDAGHALAAAGCTQIDVVPLFLGTGGHVRKDLPLQVEQLRAAHPQAVWAVRAS
                     IGEAPVVIDAIAQAALAGVSGNVLFKPGLVDSA"
     gene            complement(15804..16916)
                     /gene="lptG"
                     /locus_tag="PKV56_08555"
     CDS             complement(15804..16916)
                     /gene="lptG"
                     /locus_tag="PKV56_08555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOX67906.1"
                     /translation="MKTVRRLFYVDIVTAVAFVSLAFLSLFYFIDFVDALSDSAKRGS
                     SVLTAMATCLLEVPGHFYELAPIAVLIGTIYALSRLAQSSEFTILRTGGLGPGRALRL
                     LATLGLSFGALTFFVGDYLTPLAESRAVQLKALTRGGIALGRSGTWLKDRQTTPEGER
                     SYSINVARTGTKGEMLGIKIFEFDGDARLIRQVAAESGVVEGQSTWTLSNVTLTRWIP
                     AVASQPPQVSTERLPRWQWNGGLSADVVASALLPLATMSTLELWRYIGHLDTNEQAAQ
                     AYEIQFWKRALYPFACLVMVGLALPFAYLHARAGGISLKVFGGIMLGIAFVLLNNLTG
                     HLGLLRNWTPWIVAAAPSALFLLMSMAAFAWLVRYR"
     gene            complement(16913..18022)
                     /gene="lptF"
                     /locus_tag="PKV56_08560"
     CDS             complement(16913..18022)
                     /gene="lptF"
                     /locus_tag="PKV56_08560"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOX67907.1"
                     /translation="MLFDSTLRRDLARSFGATLVVILTIVLTMMLIRTLGMAAGGKVS
                     PQDVVLLLGYTALAHLPTMLALSMFVAVVGTLGRMYRDSEMTIWFASGVSLSRFVRPV
                     FRTTWPVLLVVLLLALFVWPWVNERSSTLRDQFDRRSDLSRVAPGQFQSSSDGNRVFF
                     IERDSQDSSNARNVFIYGRLKGAESVTTARTGRVEVEDENRYLELGNGQRNEVDLKSS
                     EKTLAQFQSYKVVVGDRLVSSADNLPPKARPTRELWRKATPAYLGELTWRLGLSLAAA
                     NMVLLAIGLAAINPRRASNWNLLFALLTFVVYYNVITLSQAWVASGKLDMGTALIAAH
                     GGALMLALGLIWWRDTATSRPNWIQASASRRLATR"
     gene            18050..19510
                     /locus_tag="PKV56_08565"
     CDS             18050..19510
                     /locus_tag="PKV56_08565"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOX67908.1"
                     /translation="MDFRHLVAAPATLSTIAADALLLVVHGNALDACVEPALAALIDE
                     AVQAGDFQFKAGRTLYVHRPVGFKAARLMVVGAAAASVKSFKAAVAAGLGQLKGTGAK
                     HIVVAVADSLPLTALHGEALAACANEATYVYRHTKPSAPKAPAMARFTLVCAKAQARD
                     VAAGLQRGSAIAAGVTLARECANRPGNVCTPTFLGEQARRLGKEHGLKVEVLDRKAVE
                     KLGMGAFLAVAQGSSEPLRFIVARYEGAAKSQAPVVLVGKGITFDTGGISIKPSAEMD
                     EMKYDMGGAASVLGTLRAVAEIKPKLNVIGIIPSCDNMPSGHAVKPGDVVTSLSGQTI
                     EILNTDAEGRLILCDALTYAERFKPAVVVDIATLTGACVIALGHHRSGLFTADDALAT
                     ELLNAGDAAIDPCWRMPLDDEYDEGLKSNFADMGNVGPRAGGAITAAMFLKRFTAKYR
                     WAHLDIAGTAWKSGAAKGGTGRPVGLLTHFVLSQVK"
     gene            19534..19965
                     /locus_tag="PKV56_08570"
     CDS             19534..19965
                     /locus_tag="PKV56_08570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HOX67909.1"
                     /translation="MTAVQFHVNVRDPLQYTCRLLRKAARMGARVAVTGTPARLAELD
                     RALWVFEAEEFLPHAVVLDAASLTSAQRRAPVWLVRQAELGADRSVLVNLGADVVAGF
                     ESYERLIEIVSADEDERDAGRRRWREYQARGYSPAKHEATA"
     gene            19962..>20232
                     /locus_tag="PKV56_08575"
     CDS             19962..>20232
                     /locus_tag="PKV56_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67910.1"
                     /translation="MSVATLPPMRVPTLTEVVEWPPSLLEGSAIPGSPDAEQLPVLSE
                     AVQPQEFAPSTGEAEVEPVVESIELEAEAEAEAEAEAEAEAEAEAE"
     assembly_gap    20233..20332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20333..20604
                     /locus_tag="PKV56_08580"
     CDS             <20333..20604
                     /locus_tag="PKV56_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67911.1"
                     /translation="SVPTMPALTEAIDEAALTRRIVGELQHRVDLALEDQLRAALAPM
                     LDRLMHTLVREARDELAPTLRDVVAKAVSHELEQHRYAPDRADPD"
     gene            20697..21839
                     /locus_tag="PKV56_08585"
     CDS             20697..21839
                     /locus_tag="PKV56_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX67912.1"
                     /translation="MQFKLNVGLFAVAAACAGSVSAQDLVVRIGHVAPTSGGIAHLGK
                     DNEMAARMAIDELNARGLSIGGKKARFELLAEDDAADPKQATAVAQKLVDSKVNGVVG
                     HLNSGTTIPASKIYNDAGIPQISPSATNPKYTRQGYKTAFRVVADDVHLGGTLGKYAF
                     GTLKGKSIAVIDDRTAYGQGVAEEFVKGVDGSGGKVVARQFTTDKATDFSSILTSIKA
                     SKPDVVFYGGMDAVGGPMIRQMKQLGIDAKFMGGDGLCTAELPKLSAGAMGDGQVVCA
                     EAGGVEGEQKKGMDDFRVAFKKKFGVDVQLYAPYVYDSVNLMAAAMVKAGSADPAKYL
                     PVLAKTEGYKGVTGTISFDAKGDIKNGALTLYTYKAGAREQIAVVR"
     gene            complement(21903..>23644)
                     /gene="glgX"
                     /locus_tag="PKV56_08590"
     CDS             complement(21903..>23644)
                     /gene="glgX"
                     /locus_tag="PKV56_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOX67913.1"
                     /translation="FDRQHPSRMDHRDNAALALKARVVVDAFDWGGDRSPRIAPVDTV
                     IYEVHARGYSRLNPRIPQPLRGTYAGLAHAASIAHLRALGVTSLCLLPVHFHLDEERL
                     VAMGLRNHWGYNTLGFFCVEPRYASAVCGQSPRDEFKSMVKALHAAGFEVLLDVVFNH
                     TAESDECGPNLSFRGLDNASYYRLPPESRGLYENHAGCGNTLDIRQPAVLQLVMDSLR
                     HWVTDMHVDGFRFDLAPVLGRGDHGFHRDGAFFGAVAQDPVLSRVKMIAEPWDIGPGG
                     YQVGAFPRGWQEWNDHFRDAMRTFWLRTDIPSNHRATFARRLCASSDLFQQRRRSPLE
                     SINYVVSHDGFTLRDLVSYAERHNLANGEGNRDGHGHNLSINAGVEGPSDDPGVNALR
                     GRLQRALLATTLLAQGTPMVAAGDEIGHTQRGNNNPYCQDNEITWIAWDQADADLFAF
                     TARLLELRRNALPFGGDWYSGLTDPFGLHDLTWLRASGDAMDSQSWHDDASRVLGCLI
                     GRPGRARAPLLLLVNGDTTPCDFMLPAGVWQGLLDTAHPRGASRWHGQGEAPILLSAH
                     SLMLLAAAGAGVA"
BASE COUNT         4060 a         7500 c         7709 g         4275 t
ORIGIN      
        1 aggcgcaggt tggcctcgat catctccttc ttggcgtcgc gcgaggcctt ctcgccctcg
       61 ttcatgcgct tgttgatctt tttcagatca tcgagcggca cgacggcacg cgcctgctgg
      121 tcggtcagct tcttctgcag ttcctgaacc ggcggcaggt tgcgcgccag cacgacgctg
      181 tagggcttgt tggccgccac ttccttttcc gcccatttca gattgaggat gttcggcggg
      241 aaatgcttga tgaaatagtc ctgcggcatg ccgcacttgt cgacgaggat ctttcgcagt
      301 tcgcgttcgt agcggcgcac gtcatcgacc tgagaacgca ggatgccgca cagcttctcg
      361 atggtcttga cggtgaagcg gatcgtcatc aactcgctgc tgatggcctg ctgcgccttg
      421 ctgtaggcgg gcgactggta gccctccttc tcgaaggcct tgcgcatctt gtcgaagttg
      481 atccgcaggt tctcgaactt tgccagggct tgggtcttca gttcctcgag cttcttggtc
      541 agcgccttgc tgccgccgtt gccgtcgtca tcgtcgtcct cgtcgaagaa gtcgacgtct
      601 tcctcggcca cgtaatcgtc ggcttcgccc tcggatacga atccgtcgac cacctcggag
      661 atggtcattt cggctgccgt gatgcgatcg gcgtagacca ggatctcggc gatcgtggtc
      721 ggcgatgcac tgatggcgta catcatggcc tgcaggccac cttcgatccg cttggcaatc
      781 tcgatctcac cctcgcgggt gagcagttcg acggtgccca tctcgcgcat gtacatgcgg
      841 accgggtcgg tcgtgcggcc gaactccgag tcaacggtcg acagcgccgc ttcggcagct
      901 tcctcggctt cctcctcggt cgcggtcgtg gtgcccccgc cggcgatcag cagcgtggcg
      961 gcatcgggtg cctgctcgta gaccgcgatg cccatgtcgt tgagcatcga cacgatggct
     1021 tcgagtattt cgttgtcaac cagcttttcg ggcaggtgat cgttgatttc ctggtgcgtc
     1081 aggaagccgc gcgtcttgcc catcttgatg agcgttttca gctcctggcg gcgcttggcg
     1141 acttcttcct cggtgagagc cgtctcgtcg agaccgaact cacgcatcag cgcgcgctcc
     1201 ttcgcacgcg acaccttcat gcgcagaggc ttgaccttga ccttcgagtc cccgtcgtcg
     1261 gccgactcgg cgacctcggc ttccggttca ccctcgagat cggcttccag gtcggaaaag
     1321 tcctcgtcac cgagttcggc cttcttgctc ttgtcgtcgc ccttggctgg cttgcgaccg
     1381 cgcttgggct tgtcgtcgcc cgctcccgcc ttggccgttg ccttggctgc cggcttcgct
     1441 cccgcggcag atttgactgc tgtcgccgag gcacgcgtct tcgcagccag ctcctccttg
     1501 gcgccgctgg atttcgaggc ggcacccttc accggagtgg cgggcttggt ggccgctgcg
     1561 ggcgcgcttt tcttcgcagt catgcaattc ctcaataggt cggcggttca acgccggtac
     1621 ttcaacaagt ggtcccgcag gtgtcgactc acaagatcgt tgcggatcgg cggttgagtt
     1681 cgcaactcct gatggagcgc tgtttcaaca tgtgagtcag cttgccaagg gggcgcggag
     1741 gccctcagac ggtttgaaag gtgatccttg cggtgttcga atgtccctgt cgccacaacc
     1801 gagatcaggg atcagacggg gaccgaaggc tccgcagtca ctgccgaccc agtcggcgca
     1861 gccgaggatt ataccgcccg gtcatcgcga accgaggctt tgagccgctt gcgaggccgg
     1921 atgcaatcgt caagccggcg acgatgcgag gccgctcaac gtcgccttca accgggctcg
     1981 gctctgcatc agttctgcgc ttcgggcctg gatgtccggt gacatgagac cggactcgaa
     2041 gagttgctcg agttcggcat ccacgtcgcg cagccgaagg tgatcgagga tcaggtcgag
     2101 ctgctggcgc aggtcggtgt gaggctcggg ctcgtggaac agcgcgatcc gctccagtgc
     2161 cgctcgggca tgttcgtctt cgtgcaccga ttgcgtcagt tcggccagca gcggggccgg
     2221 ggccagcggc ccatggtcgt ggaggcagcg ctcgaaactg ccgaaaagcg cagcgtaggg
     2281 cgccacgcgg tcggccagca agtcgtggca atgtccgtcc agatcccacc acatttctgc
     2341 gcgatgcagc agcaaccaca gcgcccgatc gagctgattg gccgcaccct ggcgagccgc
     2401 gcgaacgaga cgcggcctgg gcggcgtctc gttgcgcgcc ggctgtctga gtgtgggagg
     2461 tgaggcctgc cacatcacgg acagttctgc cgggtcgagc ttggccacaa cggccagatc
     2521 tccaagcagc tgtcgcttca acgcgccatc cggcagtgcc gtccacaagg gacgcgccat
     2581 ggccaggaat cgggctcggc cttcggctgt cgccagatcg catccttcct gggccatcgc
     2641 gacgacctgg cgcgacaagg ggacagcctg tcctatgcag gcagcgaaag cctcggtgcc
     2701 atgctcgcga acatatgaat ccggatcgtg ctcggccggc aggaacagga atcgcacgct
     2761 gcgcaggtcg gtggcatgag gcagcgcagc ctcgagggcg cggccggcag cgcgccttcc
     2821 tgcagcatcg ccgtcgaagc tgaagacgac cgaatccgtg aagcggaaca gcttctgcac
     2881 gtgttcggca gtgcacgcgg tgcccagcgt ggccacggcg ttcggaaaac ccagctgggc
     2941 gagcgccacg acgtccatgt agccttcaac gacaaggacg aagccgtgct gccggatggc
     3001 ggtgcgagct tcgtagaggc cgtacaactc gcggcccttg ctgaatacgg gtgtttccgg
     3061 cgagttgagg tacttgggct caccgcggtc gagcacacgc ccgccgaaac cgatcacctc
     3121 gccctgcacc gatcggatcg gaaacatgat ccggtcacga aaccggtcgt agcgcttcga
     3181 gtcggccccg ggagtcgcct ctgcgtcctg cgcgatgacc agaccgcttt cctcgagcag
     3241 cgggtcgtcg taccgcggaa atgcgccagc cagtgtgcgc cagccttcgg gggcgtagcc
     3301 catggcgaat cgggccgcca cctcgccgct caatccgcgc tgcttgagat agtcgatggc
     3361 ccgcgggcag ctcttcaact gcttgcggta gtggtccgcg gccttggcca gcacatcgtt
     3421 cagcgtgcct tgccgctcct tggtcttctg cgcgcgctcc cgctcctcgg gggagcggtc
     3481 gtcctgcggg acggcgaggc ctacctgctg tgcgaggtct tgcaccgcgt cgacgaagcc
     3541 catgccggag tgctccatca ggaagccgat cgcgttaccg tggacgccgc aaccgaagca
     3601 gtggtaagtc tggcggctgg ggctgacgga gaagctgggc gatttttcgc cgtggaatgg
     3661 gcacaagccc atgtggttcg ccccggactt cttcagcttc acgtagcggc cgacgacgtc
     3721 cgcgatgtcg actcggccga gaagatcctg aatgaacgaa ggcgatatca cgccttcagt
     3781 ttagcggtcc ggcaccaggt caatgagccc gcagatctgc aaggcgtcca agaaaaatgc
     3841 cgcgcagacc tttcaggcct tcgcggcatt cggcggcgac gctcggccga gactcagaca
     3901 gtgaactcgc tcaactggtg acctgccgac aaggcagcct tcagccactt gggttgcagg
     3961 ccacggccac tccaggtttc gccggtcgcc ttgttcctgt acttggcagc gaccttgccg
     4021 cccttggttg aactcgcggc acggaccggc gccttgttgg agatatccga gatggtcaga
     4081 ccgtattcct gcatcaaggc acgaaccttt tcgatggcgt tcgcatgctc ttgcttgcgg
     4141 gtcgactcga tttctttgtc caacgctgcc ttgcggtcca gcaattcttt caatgtcgac
     4201 atggggattc ctcttgggtc aattaatgaa ccgtcgaata acgacaagcg gttgcagtat
     4261 acaaaaatcg acttggaggt taaaccctca ccccggaaca cctgttaatt aatccataaa
     4321 acgacatgcg gtgacaaatt cccatcgtcc gcaatgcaaa tcttgaatcg ggttatgcag
     4381 agggcggtac gtaaccggcc ggctgctcgg caccggtgcc gaaaaagaaa ttctccattt
     4441 gacgtgccaa atactgccgc gcccggaggt cagccagatt cagtcggttt tcgttcacca
     4501 gcatggtctg gtgtttcaac cacaactgcc aggcttcctt gctcacctcg ttgtatatgc
     4561 gcttgccgag gtcgcccgga tacggcgcgt agtcgagtcc ttccccttcc ttcttcaagt
     4621 ggatgcaggt gacgatgcgt gccatgtgtt aactccgata aagatggttt caacgaactg
     4681 tggtgctgtc atgcatgggc gttgccatca ggacagcgtc ttgaccagaa cctgcgatct
     4741 tcgatcccag ttgtactttc tctggcgcgc ctctggcagc cattccggcc caacctgctc
     4801 gaatccgcgc ttgatgaacc agtgcatcgt gcgtgtggtc aacacgaaaa tgctcttgag
     4861 accgagggca cgcgcgcgct gttcgatgcg cttgaggatg cgttccccgt caccttgccc
     4921 ctgcacgtcg ggcgacaccg tgagggccgc catttcgccc gtctgctttt ctggataagg
     4981 gtatagcgcc gcacatccga atatcacgcc gtcatgttca atcaccgtgt agaaatcgat
     5041 atcccgttca atttctgttc ggtctctttt caccagcgtg ccgtcgtttt cgaaaggctc
     5101 gatcagttgc agcacgccgc ctatatcgtc ggccgtcgcc tcgcgcaggc tctcgagttt
     5161 ttcatcgacg atcatcgtgc ccacaccatc atgcgtgaat gattcgatga gaaccgaacc
     5221 gtcgacagaa aacggaacga tgtgcactcg gtccacgccg ctcttgctgg ctttcactgc
     5281 gtgctgcaga taaaacgcgg tgtcggtggg ttgctgtgga ttcggcaacg aggccagcag
     5341 tttctcggca tcggccagcg cgagctcctg gtctatggcg ctgtttggat ccgagatatc
     5401 gagaggtatg cctctgacct cggtgagata aatcaacttg tcggcctgca atgaaattgc
     5461 cgtgcttgct gcgacgtctt ccatgctgag gttgaaggcc tcgccggtcg gcgagaatcc
     5521 gaacggagac agcatcacga gtgcaccgaa gtcgatggcc tgccggatgc cgactgcatc
     5581 gatcctgcgg accaggccgg tgtggatgaa gtcgactcca tcaatgacac ccaccggttt
     5641 cgcagtgatg aagttgccgg acaccacgcg gatctggctg ccggccatcg gcgtgttcgg
     5701 caggccttgc gagaatgcgg cctctatctc gtagcgcaac tggcccgctg cctcttgcgc
     5761 gcaatcgagc gccaccgcgt cggtgacgcg catgccgtgg ctgtacttcg agacatggcc
     5821 cttggccttc agctgctcct cgacctgcgg ccgaaagccg tgcaccagca cgaccttgat
     5881 gcccatcgcg tggatcaacg cgaggtcctg cgcaaaggcg ctgagcttcc ccgcggcaat
     5941 cagctcaccg gcgatggcga ccacgaaggt cttgctgcga tgggcgtgga tgtacggcgc
     6001 gaccgaacgg aaccacggaa caaacgtgtg cgggaagacc aggctcatcg cggcgctttg
     6061 aaataatgtt cgattttgca tgaaggcccg atgacgtctg ccccgcgagt ccgctcgacc
     6121 ccccgtccga acgcgctccc tgcgaacccc atcccgccca tcacctttgc ggattcgctg
     6181 cccgtctccg ctcgccgcga cgaaatcgcg accgcgatcc gtgatcacca ggtggtcatc
     6241 gtctgcggcg agaccggttc gggcaagacc acgcaacttc cgaaaattgc actgaccctc
     6301 gggcgagggc ggggcgcggg cgggagcggc ctgatcggtc acacgcagcc gcggcggatc
     6361 gccgcgtcga gcgtggccaa gcgaatcgcg caggagctca attcgccgct cggcgaagtc
     6421 gttggctaca aggtccggtt ccaggaccgt ttgtctcccg gagccaccgt caagctgatg
     6481 accgacggca tcctgctggc cgaaacccaa accgatccgc tgctgcgcgc atacgacacg
     6541 ttgatcatcg acgaggccca cgagcgcagc ctgaacatcg acttcctgct cggctacctg
     6601 cgtcagttgt tgccgcgtcg gcccgacctc aaggtgatcg ttacctcggc caccatcgac
     6661 gccgaacgat ttgcgcagca tttcgccggc aggaacggac cggcaccggt gattcaggtc
     6721 tcgggccgac ttttcccggt cgagcagcga tggcgaccct tcgaggacag ccgagagtac
     6781 ggactcaatc acgcaattgc cgatgccgtc gacgaactgt gggcgtcggg tggagccggc
     6841 tcgggtggcg atgtcctggt gttcctgcct ggcgagcgtg agatccgcga tgcggccgac
     6901 cacctgcgcc gccaccatcc gccaggcgtc gaggtggtgc cactgttcgc gcggctcagc
     6961 caggccgagc aggaccgcat cttcgagccg cacagcgcac gccgcatcgt gctgtccacc
     7021 aatgtcgcgg aaacgtcgct gacggtgccg ggcatccagt acgtgatcga cgcaggcacc
     7081 gcgcgggtca agcgctacag ctaccgcaac aaggtcgagc aactgctgat cgagccgacc
     7141 agccaggcgg cggcgaatca gcgtgccggt cgctgcggtc gggtcagcaa cggcatctgc
     7201 atccggctct acgacgagaa ggattttgcg ggtcgccccc gtttcaccga cccggaaatc
     7261 atgcggtcgt cactggccgg cgtgatcctg cggatgaagg cgctcgggct cgggctggtc
     7321 gaggacttcc cgttcatcga gccgccgccg cgccgcgcca tcgccgatgg ctaccagttg
     7381 ctggccgagc tcggcgcggt cgacgatcag aacgagttga ctccgatggg ccaggagctc
     7441 gcccgcctgc cgctggaccc gcgggtcggc cggatgattc tcgaggcgcg caatcgacag
     7501 gcgctcaccg aagtgctgat cgtcgccagc gcgatgagcg tgcaggacgt gcgtgatcgt
     7561 ccgctcgaag cgcagcaggc ggccgatgcg gcgcacaaga agttcgacga cgagcgctcc
     7621 gagttcctcg gggacctgaa gctgtggaaa tggctggagg cgtctcgcgg cgcaggcctg
     7681 accgccgaac agcgcgaaca caagctcagc gcgcgcaagc aggaacagtt gctgcgcgaa
     7741 aacttcattt cgccacgccg tgtgcgcgaa tggcgcgaca tccactcgca gttgctgacc
     7801 gtggtcgccg agcacggctg gcgactcaat gcgtcgccag cgacctatga gcaactgcac
     7861 ctctcgctgc tggcgggcct gctcggcaac atcggtgtca agagcgacga ggaagactgg
     7921 tacctcggtg cgcgtggcat ccgcttccac cgccatcccg gcatcaacct gtctaagaag
     7981 ccgggccgct ggatcgtcgc ggccgagctg gtcgaaacga cgcgactcta tggccgtggc
     8041 atcgcggcga tcgaaccgca gtggctgccg cagatcgccg gccatgtcat caagacccaa
     8101 ctgctggagc cgcactggga gaagaaggct gccgaggtga ttgctctgga gcgagccacg
     8161 ctctacggca tcgtcgtcta caacaaccgg cgggtgaact ttggtctggt caatccggcc
     8221 gaggcgcggg agatattcat ccgggaggcg ctggtcgccg gcgactggga gacccggctg
     8281 ccgttcgtcg cagccaatcg caagctgatc gctcaggtcg aggagctgga gcacaagtct
     8341 cgtcgtcagg acgtgctggt cgacgatgaa ctgatccatg ccttctacga tcagcagttg
     8401 ccggccgagg tctgcagcgg cgcgacgctc gaacgctggt accgcgacga gatcaggcgc
     8461 cagccgaagc tgctgatgct gactcgtgac gagctgatgc gacacgaggc ggccggcatc
     8521 acgaccgccg cgttccccaa gaccctgcgg atgggcggcg tcgattgcgg cgcgatttac
     8581 ctgcacgagc ccggcgacgc gaaggatggc gtgacggtga cggttccgat ctacgcgttg
     8641 aaccaggtca gcgaggagcg ctgcgaatgg ctggtgcccg gcatgctgaa agacaaggtg
     8701 ctggcgttgg cgaaaagcct gcaccagcgg ccgcgctcgc gtctcgtgcc tttgccggag
     8761 tacgccgctg ccttcatttc atctgtggag gccgcccatg gatggggcag tggcagtctg
     8821 gtcgatgcct tgctgaagca tgtccgcgac aggacgcaac tcgacgtgaa gcgtgccgac
     8881 ttcaagctgg agcaggtgcc gccacacctg cacatgaact tcagggtcgt cgacgagaac
     8941 gggcgtcagt tgggcatggg tcgcaacctg tcgaccttga aagccgagct cggcagccag
     9001 gcgcgctccg ccttccaggc gctggctcaa ttgaagagcg tccagcctga gaggccgatc
     9061 gctgccgaca ccgcgtcgtc ggtctctcgg aaacccgggg cccaacgcgt tgccgaggca
     9121 cctgctgtgt cgccggcagg gcgcgcttca gtcccacccg acagcgcagc ggagcactac
     9181 accgactgga ccttcggaga gctgcccgaa ttgctggagg tccgccgcgg tgaccagacg
     9241 atggtcggat ttccggcgct gatcgaccgc ggtggcgccg tggtcatcga ggtgtttgac
     9301 gagcccgagg cggctgcggc acgccatcgc gagggcctgc gccggctggt gtctctgcag
     9361 atccgcgatg cattgaagta tctcgagaag aacattccag acctgcagaa gatgtccgtg
     9421 ctctacatgc cgctcggaac ggcagatgaa ttgcgtgagc aggtcatctc gctcgccatc
     9481 gatcgagcgt ttcttgtcga tcccctgcca accgatgcga ttgctttccg gcgtcgtgtc
     9541 gaagaggggc ggggccggct cacgctgatc gccaacgaga tcgcgcgcag tgcgcaggcg
     9601 gtgctcgtcg atcacgcggc ggccttgcgc aagctcaggg acgcccgcgc tccaaaagag
     9661 gtgtcggagg acatcgaggc gcagttgtcc aggctggtcc ccaagcgatt cctgggcgcg
     9721 acgccctggt cccagctgtc gcacctgccg cgctacctgc gaggcatcgt catgcgtctc
     9781 gacaagtggc gtgccgatcc tggacgcgac gcatcccgga tggccgaatt gcgaccgctc
     9841 gagcagcgat acctgcgaac cgtgatcgag cgcaagggcg tgcgcgacac gcggctcgac
     9901 gagttccgct ggttgctcga agaactgcgc atcagtctgt tcgctcagga gctgcgcacg
     9961 ccgcagccgg tcagcgtgaa gcgactggag aagtcctggc tgcagatgac gcagtgaccc
    10021 ctgaccatgt cgttgctgag attcatcgaa gaaaattgtc gcctcctcga cgaggcacca
    10081 tttgccggca tacaatgcga ggctcggggc gtagcgcagc ctggtagcgc acctggtttg
    10141 ggaccaggtg gtcgtgagtt cgaatcccac cgccccgacc atattcagat tcaacctcag
    10201 cagtcttcgc tggggctctc cggtgtcacg ttcctggtcc ggcttggttg tctgaacttc
    10261 tgagccgccc agtccagtgt caacggtggc ggtagctcaa ccggatagag caccagcctt
    10321 ctaagctggg ggttacaggt tcgattcctg tccgccgcgc cccgccccgt cagtgctcgg
    10381 ccgcctcgcc tcgaggccgc cgaatacact gggaggctat ggaagccctg gtccacttcg
    10441 cccatccgat tcgccacgcc gtatcaggcg ctgaggtgcg tgcatgttcg gtgcattgaa
    10501 gcgtttgttg gcgctcggtc ccgggcgagc cggaggtgcg cgcttcgacg cgatcgattc
    10561 ctgggcggat cgctctggat tccggtttcg cagggccaag gacggtctgc gattcgagct
    10621 cgaggcggca tccggcggac acgtggttcg gatggagtgg gggccgtccc aacgcagcta
    10681 catcgagcac agcgagttgc gcatgcgcgt cgacatgaat ctgcccggtt cgttgcagat
    10741 gctcgtgctg acgctgacct tgaaggaacg gctggagtcc gaaaccttcg agcgctacac
    10801 gcaggacgcg cagaccgtca tcgacatgtc gaccccggaa gagatgcgct ggctggcgat
    10861 gtttccgaag gcagatctgt cgttcgacac ggtcttgcgg gagcgttttg gtgcgctcgg
    10921 ggcgaacccg gcgtgggcgc tggcctggat caacggtccg ctcggtgcgc aactcgcccg
    10981 cgcggtgccc gatctgatcg ggccggatgt cccgttcgtg ctgatgacaa tgcgcggcag
    11041 gatctacctg cgcatgcagc tcgccgaccc agcacccgat gcgctttccc agtgcctcga
    11101 ggtgttcgat tcggccatgc ggtcggtcca gcagatcacc ggccagatct cgccggacga
    11161 cgccgaatgg gcgtcgaccg ccagcacgtc ctggcagacc cagttgcccg acgacgaagc
    11221 gacggatccg acacgctttt gacgtgtgtc acatgactct gctccggcag gtgcgtgcta
    11281 gagtcatttt cgttacgttt ctttacggcg agcatgctga aagcaataac gactgtcgcc
    11341 gcggggttac ttctggcctt gtcatcgcag cccctgatgg cggtgggttt cggtcgtttg
    11401 gtcaacgcca cttcgctggg acaggctctc gacgtgagcg tggctctcga cgccgacatc
    11461 accgagcaag tcacgtctga ttgcgtcagc gccgaagtgg tggtcggcga cagtgtcctg
    11521 ccgcgcccgg tggttcgggt tcgactcgaa ccagctggca atgcgagtac gcgttccttg
    11581 cgcgtgacga ccacggtgcg catccacgaa cccatcgttc aggtcacggt cgcagcggcg
    11641 tgcccgtcgc gagtcagccg acagttcgtg gttctgatcg atcctcctct gactgcgtcc
    11701 gagcccgcac tcgcagcggc tgagccacct gctgtggcca cgcctcccgc atcccccgcg
    11761 acttcggtgt ctgtcggagc actgccggat ccggggtttg cacagggcat ctccgacgcg
    11821 gctgctggat cgggggtgct ggctccaccg ccagcgccgg cgccgccggc atcagcccgt
    11881 cgcgcgacgc gatcgacatc gaaaggcgtg gcgagcgggg cgaaggcggc ccgtcgggcg
    11941 caggcagcca ccccgcgcaa ggctcgacga gcatccgtca accaggcgcg ggcgcgctct
    12001 tccccgcgcc ttgaactcga gcgcggcgcc ggcccactgg ccgctgctgc agcctcagct
    12061 ccgctggcgg tggctggtgt gctgccggtg gctctcgccg cgagtgctgc cgcatcgagc
    12121 cctgccgagt cagtggccga actgcagcgc gaggcgattc aatcgcttca gcaaagactc
    12181 gacaagttgc aggccgattc cgaagcgtcc agcaggagca tcgccgattt gcaggctcgc
    12241 ctgcgtgctg ccgaatcgtc acgctccaca ggatggcttt catatgcact cgcagcggcg
    12301 ggcgccgcgc tgctgctgtg gtttggtcag atgatcgggc gccgcgctgc tgcacggcgt
    12361 cccggcacgc cttggtggaa ggccgaagca gatgcggctg cggcgaagcc ggtcgatgcg
    12421 gaggaccgcg catccgccag gccggcgttc gtgcagagcg ccctggcgag cctgccggag
    12481 gccatcgaac tgcctgatga ggtgctgccg accaccccgg ctccgctcgt cgtcccagat
    12541 cattcgcgac tgcccgagcc cttgcgtcct gtgcaggttt tcggcgacac ggtggcgtcg
    12601 gtcggaacgg ctctggcggt tgagccgccc gtggccccgg cgtccgcgct gacagccgat
    12661 gaactgatcg acctggagca acaggcggag ttctttgtgg cgcttggtca ggacgattca
    12721 gcggtcgatc tgctcaacgc cttcctgcgc gggggcggtg gcgcgagtcc gctgccctac
    12781 ctgaagctgc tggaaatcca tcggcgtcgg gaagaacgcg acgcttacga gcggatccgg
    12841 gatcgccacg cgagacgttt cggtaccgaa gcgccgaagt gggaggacca gacggcagcc
    12901 gccgttctcg cggacggaca gtcaccggtg atgcgtcaga tcgagtcggt ctgggcagat
    12961 ccgcccgagg cgatgaagct gatcgagtcg ctgctcgtga gcagcgaccc ggcagcggcg
    13021 cccctcgacc tggcggcctt gaccgatctc cagttcctct acctgcttgc ccgggcggtt
    13081 cgcgaattcg aggatcagcc cgcccagcct tctgccgagt cgacggtgga cctgctgctg
    13141 cccctgtctc atcaagacga acaccggtcg gtggtcgcct cggtcgattc gggcttctcg
    13201 actccgtctg acatcgacct cgagctggat ttcctgccgc cacggaacga cccgaagccg
    13261 ctggcctgag gccgtggcgc tgcaggtcag agcctgcgca atctctccag cgcaagttcc
    13321 agcgtggtgg tctgcttggc gaagcagaaa cggatcagcc gctgctcgag cgcatcggca
    13381 tagaaagccg acatcggaat ggcggccacg ccggtttcgc gcgtcaatcg ctcgcagaac
    13441 gcgcgctcgg gttcatcgct gatgctggag tagtcggcgc actggaaata cgtacccgcg
    13501 ctgggcagtg gccgcaggcg ggtcgcccgc aggccatcgg cgaaaagatc acgcttgcgc
    13561 tggtagaaca tcgccagccc gaggtgaggc ccagggtctc gcatgtaggc agccaggccg
    13621 tgctgcaccg gcgtgttgac ggtgaacacg ttgaactggt gcaccttgcg gaactcggtg
    13681 gtcaacgcgg cgggagctgc cacgtagccg accttccagc cagtcacgtg gtaagtcttg
    13741 ccgaagctcg acacgacgaa gctgcgcgcc gccagctccg gccagcgggc gatgctctca
    13801 tggcggatgc cgtcatagac catgtgctcg tagacctcgt cgccgatgac gagcacgtcg
    13861 gtcgggcgca gcaagtcggt cagctgctgc atttcggcgg ctgtccacac cgtggcactc
    13921 gggttgtgcg gcgtgttgac gacgatcaga cgggtgcgcg gtgtcagtgc ctgtgcgata
    13981 cggtcgaagt cgggccggaa gctgcctgga gtgagcggta catgaaccgc cactccgccg
    14041 gccaaggcga cgttcggctc gtagctgtcg aagcacgggt cgagcaggat cacctcatcg
    14101 ccgggatgaa ccacggccag catgatggtc aggatcgcct gcgtggcacc cgcggtgacc
    14161 gtgatctcgc tggctgggtc gtatcgacgc ccgtagagcg actcgatctt gctggcaatc
    14221 gcttccctca gggcaggcac acccggcatc ggtgggtact ggttgtggcc gctgcgcatg
    14281 gcgtgattca cggcatcgag cagtcgagga tcgcattcga aatcaggaaa gccctggccc
    14341 aggttcacgg cgccgtgctg ttgtgccagc gcggacatca ccgtgaagat cgtcgtgccg
    14401 acctgcggca ggcgactggt tactggaggc gtgcggtacg ccgtcttggc gtgggtgccc
    14461 aggctcattc ggtcgatggc acgccgttga tgctgcgggt gatcagggcg cggctcagac
    14521 gctcggagat catctcggcg aacacgtaga cgaagttgcg caggtaggca ccacgcttga
    14581 aggcgatgcg tgcgacgttc tggccaaaga gctggccggc cgggcgccac accagatccc
    14641 cgccgggcgg atcgtcgcgc actgccattt cggcgacgat gccgatgccg agcccgagcc
    14701 ggacgtaggt cttgatcaca tcggagtcga tcgcctcgag cacgacgttc ggccggagcc
    14761 tgcgcgactc gaaggcctga tcgatgcggg tgcggccggt gaaggtcggg tgatacgaga
    14821 tcaatggctc gacggcgagc tgctccagcg tgagtcgttc gacctgcgcc agcgggtgcc
    14881 cggcgggcat cacgatgacg tgctgccatt cgtagcaggg cagggtgacg agttcgtcgt
    14941 agcggtccag cgattcggtg gccagtccga cctcggcgac ttcctcgatg accatgcggg
    15001 ccacctgttg cgggttgccc tggtgcaggc tgacgttgac cttcggaaag cgcttgcgca
    15061 accgcgcaac cggttcgggc agcacgtagc gcgcctgcgt gtgcgtggtg gcaatcgaca
    15121 acgtgcctgc gtcctggttc gagaactcgt cgccgatacg cttgaggttg ttgacctcgc
    15181 gcatgatgat ctcgatggcc ttcagcactt cctggccagg ctcggtgacg cgacgcaggc
    15241 gcttgccgtg gcgggcgaag atgtcgacgc caagctcgcc ttccagctcg aggatggcct
    15301 tcgacacacc gggctgcgaa gtgaacaggg ccttggcggt ctcggtcagg ttcaggttct
    15361 gccgaacggc ctcctgaacg aagcggaatt gatgcaggtt catgccgagt ccacgaggcc
    15421 gggtttgaag agtacgttgc cggacacgcc tgccagcgcg gcttgggcga tggcatcgat
    15481 gacgacgggc gcttcaccga tcgacgccct cacggcccag actgcttgcg gatgtgctgc
    15541 acgcaactgc tcgacctgca gcggcagatc ctttcgaacg tgcccaccgg ttccgaggaa
    15601 cagcggcacc acgtcaatct gcgtgcatcc tgccgcggcc agcgcgtgtc cggcatcgag
    15661 caggttcggg ctcatgaact cgaggaagca cagttcgacg cagacatcgg gtgcgcgatt
    15721 gcgcaccgat tcgagaacgg cccggaatgg cagcgaccag ttggggtcgc gcgcaccatg
    15781 ggcaaacagg agcagtccgg tcttcatcgg tatcggacca gccaggcaaa ggcggccatg
    15841 gacatcagca ggaacagcgc gctcggagcc gcggcaacga tccagggcgt ccagttgcgc
    15901 agcagtccca ggtgacctgt gaggttgttg agcagcacga aagcgatgcc gagcatgatg
    15961 ccgccgaaca ccttgaggct gatgcctccg gcccgcgcat gcaggtaggc gaacggcagc
    16021 gccaatccga ccatcaccag gcaggcgaaa ggatagaggg cccgcttcca gaactggatc
    16081 tcgtaggcct gcgctgcttg ctcattggtg tcgaggtggc cgatgtatcg ccagagttcc
    16141 agtgtcgaca tcgtggccag cggcagcaac gccgacgcga cgacgtcggc cgacagcccg
    16201 ccgttccatt gccagcgcgg cagcctctcg gtgctcacct gaggaggctg cgaagccacg
    16261 gcggggatcc agcgtgtgag cgtgacattc gacagcgtcc aggtggattg cccttcgacc
    16321 acgcccgact cggccgcgac ttgccggatc agccgggcgt cgccgtcgaa ctcgaatatc
    16381 ttgatgccca gcatttcgcc cttcgtgccg gtgcgcgcca cgttgatcga gtacgagcgc
    16441 tcgccttcgg gcgtggtctg gcggtccttc agccaggtcc ccgatctgcc gagtgcaatg
    16501 cctccgcgtg tcagcgcctt cagttgcacg gctctgcttt ccgccagcgg cgtcaggtag
    16561 tccccgacga agaaggtcag tgcgccgaac gagagtccga gagtggcgag cagccgcagt
    16621 gcccggcccg ggcccaggcc ccccgtgcga aggatcgtga actccgacga ctgcgcgagc
    16681 cgagacagcg cgtagatcgt gccgatcagc acggcgatcg gcgccagctc gtagaagtgc
    16741 ccggggactt caagcaggca tgtcgccatg gccgtcagga ccgaactgcc acgcttggca
    16801 gagtcggaca aggcatccac gaagtcgatg aaatagaaca gcgacaggaa agccagcgag
    16861 acgaaggcca ccgccgtgac gatgtcgaca tagaacaggc gccgtaccgt cttcatcttg
    16921 tggccagtcg ccggctggcg gacgcctgga tccagttcgg gcggctggtg gcggtgtctc
    16981 tccaccatat cagccccagc gccagcatca gtgcgccgcc atgtgctgcg atcagcgccg
    17041 tgcccatgtc cagcttgccg ctggccaccc aggcctgcga cagcgtgatc acgttgtagt
    17101 agacgacaaa ggtcagcagc gcaaacagca ggttccagtt gctggcacgg cgtggattga
    17161 tggccgccag gccgatggcg agcaggacca tgtttgccgc cgccaggctg agacccaggc
    17221 gccacgtcag ctccccgagg tacgcaggcg tcgccttgcg ccacagttcc ctcgtcgggc
    17281 gggccttcgg tggcaggttg tccgcgctgc tcacgagccg gtcaccgacc acgaccttgt
    17341 agctctggaa ttgcgcgagt gtcttctccg aactcttgag atccacctcg ttgcgctgac
    17401 cgttgcccag ttccagatag cgattctcgt cttcgacctc gacgcggccg gtgcgcgccg
    17461 tcgtgaccga ctcggcgccc ttgagtcgac cgtagatgaa gacgttgcga gcgttcgagc
    17521 tgtcctgcga gtcgcgctcg atgaagaaga cccggttgcc gtcgctcgac gactggaact
    17581 gccctggtgc cacgcgcgac aggtctgacc ggcggtcgaa ctgatcgcgc aatgtggagc
    17641 tgcgctcgtt gacccatggc cagacgaaca aggccaataa caggaccacc agcagcaccg
    17701 gccaggtggt ccgaaacacc ggacgcacga agcgggacag gctcacaccg ctggcaaacc
    17761 agatcgtcat ttcgctgtcg cgatacatcc ggccgagcgt gccgaccacc gcgacgaaca
    17821 tcgacagcgc gagcatcgtc ggcaggtggg cgagtgcggt gtaaccgagc agaagcacca
    17881 catcctgggg tgacaccttg ccgccggcgg ccatcccgag cgtgcggatc aacatcatcg
    17941 tcagaacgat ggtcaggatg accaccaagg tggcgccgaa gctgcgagcc aggtcacgac
    18001 gcaaggtcga atcgaataac atcgtcagcc caattgaaca gtcggcatta tggacttcag
    18061 acacctcgtt gctgcccccg ctaccctgtc gactattgct gccgacgcgc tgttgctggt
    18121 cgtccacggc aacgcgctcg atgcctgtgt cgaaccggca ctggccgcgc tcatcgacga
    18181 ggccgttcag gccggtgact tccagttcaa ggcaggccga accctctacg tgcatcggcc
    18241 tgtcggcttc aaggcggctc gcctgatggt cgtcggtgcg gcagccgcga gcgtcaagtc
    18301 gttcaaggcg gcagtcgcgg ccggcctggg tcagctgaag ggtacgggtg ccaagcacat
    18361 cgtggtcgcg gtggccgatt cgttgccact gactgccctg catggcgagg ccctggcggc
    18421 ctgcgcgaac gaggcgacct acgtctaccg tcacaccaag ccgagcgcac ccaaggctcc
    18481 tgccatggcg cgtttcacgc tggtctgtgc caaggcgcag gcgcgcgatg tcgccgccgg
    18541 cctgcagcgc ggctcagcca tcgcagccgg tgtgacgctg gcgcgtgaat gtgcgaaccg
    18601 gccgggaaac gtctgcacgc cgaccttcct cggagagcag gccaggcgtc tcggcaagga
    18661 gcacgggctg aaggtcgagg tgcttgatcg caaggcggtc gagaagctcg gcatgggggc
    18721 attcctggcc gtggctcagg gctccagcga gcccttgcgt ttcatcgtcg cacgctatga
    18781 aggtgctgcg aaatcgcagg cgccggtggt gctggtcggc aagggcatca cattcgacac
    18841 cggcggtatc tcgatcaagc cgtctgccga gatggacgag atgaagtacg acatgggcgg
    18901 tgctgcgagc gtgctgggca ccttgcgcgc ggtggccgag atcaagccga agctcaacgt
    18961 gatcggcatc attccgtcgt gcgacaacat gcccagcggc cacgccgtca agccgggcga
    19021 cgtcgtcacg agcctgtcgg ggcagaccat cgagatcctg aacaccgatg ctgaaggccg
    19081 cctcatcctg tgcgatgcgc tgacttatgc cgagcgattc aagccggcgg tggtcgtcga
    19141 catcgcgaca ctgaccggtg cctgcgtgat cgccctgggg catcaccgct cggggctgtt
    19201 caccgccgac gacgcgctgg ccaccgaact gctcaatgcc ggcgatgccg cgatcgatcc
    19261 atgctggcgc atgccgctgg acgatgaata cgacgaaggc ctgaagagca atttcgctga
    19321 catgggcaac gtcggcccgc gcgctggtgg cgccatcacg gcggcgatgt tcctgaagcg
    19381 tttcaccgcc aagtaccgct gggcccacct cgacatcgcg ggtacggcct ggaaatcggg
    19441 ggcggccaag ggcggcaccg ggcgcccggt cggtttgctg acccacttcg tgctgtcaca
    19501 ggtcaagtga tcgccgctac accgcccgac tcgatgaccg cggtgcagtt tcacgtcaat
    19561 gtgcgggacc ctctgcaata cacctgccgt ctgcttcgca aggcggctcg aatgggtgcc
    19621 cgggtcgctg tgaccgggac gcccgccagg ctggccgagc tggatcgggc gctgtgggtt
    19681 ttcgaggccg aagagttcct gccgcacgcg gtggtactcg acgctgcgtc gctcacttct
    19741 gcccagcgtc gggccccggt gtggctggtc cggcaggccg aactgggcgc tgaccgatcc
    19801 gtgctcgtca atctgggagc ggatgtggtc gcgggcttcg agtcctatga gcgcctgatc
    19861 gagatcgtgt cggccgatga ggacgagcgg gacgcggggc gcaggcgctg gcgtgaatac
    19921 caggcccgcg gatactcgcc tgccaagcac gaggccacgg catgagcgtg gccaccttgc
    19981 cgccgatgcg ggtgccgacg ctgaccgagg tcgtcgaatg gccgccgtcg ctgctcgagg
    20041 gctccgccat acccgggtcc ccggacgcgg agcaactgcc ggtgctttcg gaggccgtgc
    20101 agccgcaaga atttgcgccc tccacgggcg aagccgaggt cgagccggtc gtcgagtcga
    20161 ttgagctgga ggccgaagcc gaagccgaag ccgaagccga agccgaagcc gaagccgaag
    20221 ccgaagccga agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccagtgtt
    20341 ccgaccatgc cggcgctgac cgaggcgatc gatgaggccg cgctgacgcg gcgcatcgtc
    20401 ggcgaactcc agcatcgcgt cgatctggcg ctcgaggatc agttgcgagc ggctctggca
    20461 ccgatgctcg atcggctgat gcacacgctg gtgcgggaag cccgcgacga gctggccccg
    20521 acattgcgtg acgtggtagc caaggcggtg tcgcacgaac tcgagcagca ccgctatgcg
    20581 cctgaccggg ccgacccgga ttgagtcgtc gcaggcctcg cgggctgcgg cgatcctgct
    20641 tcgccgtgca ttgcgtcctt cgccaccctt tcttgtcatt caatcggagt cttgatatgc
    20701 aattcaaact gaacgtgggc ttgttcgctg tcgctgcggc gtgtgccgga tcggtgtcag
    20761 cgcaggatct ggtggtcagg atcggtcatg tggcaccgac cagcggcggc atcgcccacc
    20821 tgggcaagga caacgagatg gccgcgagga tggcgatcga cgaactgaat gccaggggtc
    20881 tgtcgatcgg cggcaagaag gcccggttcg agttgcttgc cgaagacgat gcggccgatc
    20941 ccaagcaggc cacggctgtc gcgcagaagc tggtggactc caaggtcaac ggtgtcgtcg
    21001 gccacctgaa ttcgggtacc acgatcccgg cctccaagat ctacaacgac gccggcatcc
    21061 cgcagatctc gccttcggcg accaacccca agtacacccg ccagggctac aagaccgcct
    21121 tccgtgtcgt ggccgacgat gtgcatctgg gcggcactct gggaaagtac gcgttcggca
    21181 cactgaaagg caagtcgatc gccgtcatcg atgaccgcac cgcctacggc cagggtgtcg
    21241 cagaggagtt cgtcaagggc gtcgacggtt cgggcggcaa ggtcgtcgcg cgccagttca
    21301 ccaccgacaa ggccaccgat ttcagctcga tcctgacctc gatcaaggcc agcaagcccg
    21361 acgtcgtgtt ctacggcggg atggatgcgg tgggtgggcc gatgattcgc cagatgaagc
    21421 agctcggcat cgacgccaag ttcatgggcg gtgatggact gtgcaccgcg gagctgccga
    21481 agttgtcggc cggtgccatg ggcgacggcc aggtggtctg cgccgaggcc ggcggcgtcg
    21541 aaggggagca gaagaagggc atggatgact tccgcgtcgc cttcaagaag aagttcggcg
    21601 tcgatgtcca gctctacgcg ccgtacgtct acgactcggt caacctcatg gcagccgcca
    21661 tggtgaaggc cggatccgcc gatccggcca agtacctgcc ggtgctggcc aagaccgaag
    21721 gctacaaggg agtgaccggc acgatctcgt tcgatgccaa gggcgacatc aagaatggcg
    21781 cgctgaccct gtacacctac aaggccgggg cacgcgaaca gatcgccgtc gttcgctgat
    21841 ctcggggctt cgtcgaggaa agggcgccgc gaggcgccct tttcacgtcc gttggcagcg
    21901 cgtcaggcca cgcctgcgcc ggcagctgcc agcagcatca ggctgtgggc gctcagcagg
    21961 atcggcgctt caccctggcc atgccagcgg gatgctccgc gagggtgcgc ggtgtcgagc
    22021 agtccctgcc agacgccggc cggcagcatg aaatcacagg gtgtcgtgtc gccgttgacc
    22081 agcagcagca gcggcgcccg ggcgcgacca gggcgaccga tgaggcagcc gaggacgcga
    22141 ctcgcatcgt cgtgccagga ctggctgtcc atcgcatcgc cgctcgcgcg cagccacgtc
    22201 aggtcgtgca ggccgaacgg atcggtcaga ccgctgtacc agtcgccgcc gaacggcaac
    22261 gcgttgcgcc gcagttcgag cagccttgcg gtgaacgcga acaggtccgc atcggcctga
    22321 tcccaggcga tccacgtgat ctcgttgtcc tggcagtacg ggttgttgtt gccgcgctgg
    22381 gtgtggccga tctcgtcgcc tgcggccacc atcggtgtgc cctgggccag cagcgtggtg
    22441 gccagaagcg cgcgctgcag ccgaccgcgc agcgcgttga cgccgggatc gtcggagggc
    22501 ccctcgacgc cggcgttgat gctcaggttg tgtccgtggc catcgcggtt gccctcgcca
    22561 ttggcgaggt tgtggcgctc cgcgtagctg accaggtcgc gcagagtgaa gccgtcgtgc
    22621 gacaccacgt agttgatcga ttccagcggc gagcgccggc gctgctggaa caggtcggac
    22681 gatgcgcaca gccggcgtgc gaacgtggcc cggtggttcg acggaatgtc ggtgcgcagc
    22741 cagaacgtgc gcatcgcgtc gcggaaatga tcgttccatt cctgccaccc gcgcggaaat
    22801 gcgccgacct ggtagccgcc cggaccgatg tcccatggct cggcaatcat cttgacgcgc
    22861 gacagcaccg gatcctgagc caccgcaccg aagaaggcgc cgtcgcggtg gaagccgtgg
    22921 tcgccacggc ccagcaccgg cgccagatcg aagcggaatc cgtcgacgtg catgtcggtc
    22981 acccaatggc gcaggctatc catcaccagt tgcagcaccg cgggttgccg gatgtccagc
    23041 gtgttgccgc agccggcatg gttctcgtaa agcccacggg actctggcgg cagccggtag
    23101 tagctggcgt tgtcgagacc ccggaagctc aggttcggtc cgcactcgtc ggattcggcg
    23161 gtgtggttga acaccacgtc gagcagcacc tcgaagcctg cggcgtgcag cgccttcacc
    23221 atcgacttga attcgtcgcg cggcgactgc ccgcagacgg ccgacgcata acgcggctcg
    23281 acgcagaaga agcccagtgt gttgtagccc cagtggttgc gcagacccat cgcgaccagg
    23341 cgctcctcat cgagatggaa gtgcaccggc agcaggcaca gcgaagtcac gccgagtgcc
    23401 cgcagatgcg cgatcgacgc cgcatgcgcc agtccggcat aggttccgcg cagtggttgc
    23461 gggatccggg ggttcagccg gctgtatccc cgggcatgca cctcgtagat caccgtgtcg
    23521 actggcgcga tccgcggcga tcggtcaccg ccccagtcga aggcgtcgac gacgacgcgc
    23581 gccttcagtg ccagagcggc gttgtcgcgg tggtccatgc gggaaggatg ctgacggtcg
    23641 aagg
//
LOCUS       DAOORV010000050        23623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2616_length_23623_cov_7.194119, whole genome shotgun sequence.
ACCESSION   DAOORV010000050 DAOORV010000000
VERSION     DAOORV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23623
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2616_length_23623_cov_7.194119"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKV56_08595"
     CDS             complement(<1..284)
                     /locus_tag="PKV56_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX67914.1"
                     /translation="MLRLKQPLADLDAWVACFRDAEIPVRAETAAALEAMRQNEDDVD
                     ANLIGEMISGDPLMTLKVMAFAAKNRSARLVTAPETVTSTLVMMGISPFFR"
     gene            complement(440..1468)
                     /locus_tag="PKV56_08600"
     CDS             complement(440..1468)
                     /locus_tag="PKV56_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX67915.1"
                     /translation="MLRRTGRIVSGVLVALLLSRIGTAKAEDKVRFATNWKAQAAHGG
                     FYQAIADGTYKRFGLDVTLLQGGPQVNNRPLLAARRIDFLLTGNLLHSFDNVKNGVPT
                     VVVAGLFQKDPQSLIAHPGQGYEKFEALKSAPLALIAKDAQFSWWQWLKATHGFRDEA
                     LRPYNYNLGPFLTNPKAIQQGYSVAEPIYVEKQGRFKPVVHLLADHGFSTYSTVIEAR
                     TDTVTRQPDVVQRFVDASIIGCVNYLYGDRKTAHALMLRDNPEMTADELESSVALMKQ
                     QGIVDSGDALTQGIGAMNPARILDFYQQMVKAGLYKSGDVDLGRVATFEFVNRKVGLD
                     VKLKLGQR"
     gene            complement(1474..2319)
                     /locus_tag="PKV56_08605"
     CDS             complement(1474..2319)
                     /locus_tag="PKV56_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX67916.1"
                     /translation="MSKTPNRTMQRDPEATRHRQGWTNSPWLPLLSVLGWLLAWEVLV
                     RVARIPHYTLPAPSLVLQTLMENFGSLAMSWWFTLKITFGALLLACLGGVLIAVVFAL
                     SAPLEAVLLPLAVVLQVTPIVAIAPLMLIYVESTTAALLLCAWIVAFFPILSNTMMGL
                     RAADPLLRDLFRLYGAGPLQRLRLLLMPSALPYFVAGVKVSGGLSLIGAVTAEMVAGA
                     AGRETGLASRILEASFRTETPKMFAALLLLVMTGLMIHAASTALSRRWLVRWHASEKL
                     SGNSR"
     gene            complement(2316..3125)
                     /locus_tag="PKV56_08610"
     CDS             complement(2316..3125)
                     /locus_tag="PKV56_08610"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX67917.1"
                     /translation="MAAAAAGSTAPSIVLRDVGKRYANGTLALHGVSFEVGEREFVSL
                     LGPSGCGKSTVLRLVAGLDVHSDGQITSPALSSAAARRAPDTAFVFQDPTLMPWASVF
                     DNVWLPLRLAGRSRSDAAPDVRRVLAAVGLEESEAAFPAELSGGMRMRASIARALVTH
                     PRLLLMDEPFAALDEISRQRLNDDLLAWWQSTALAVLFVTHSVFEAVFLSQRVLVMGS
                     RPGRIAAEVVIDEPVPRTTEFRTSVRYAQACREISQALAWASASSSDGIGA"
     gene            complement(3142..4647)
                     /locus_tag="PKV56_08615"
     CDS             complement(3142..4647)
                     /locus_tag="PKV56_08615"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOX67918.1"
                     /translation="MPIAPTYIAPSRFDDPAAALAQVRTIYETSVAHLRASLHRFVGG
                     ETLPARVRACYPFVRVHTDTVARADSRLSYGFVAGPGTYETTLTRPDLFGNYYQLQFD
                     LLLRNHQVSLEIGTSSQPIPAHFSLAEHEHLEGSLDPARRLLMRDLFDLPDLGEMDDG
                     IANGTHEPQPGKDGKIRQPLALFTAPRVDYSLHRLRHYTGTAPEHFQNFVLFTNYQFY
                     IDEFVRLGHTAMSDPASEYCAFVEPGGVITRRRGLNVQQGDELGAPPPRLPQMPAYHL
                     RRDDGAGITMVNIGVGPANAKTITDHIAVLRPHVWLMLGHCAGLRNTQQLGDYVLAHG
                     YVREDHVLDEDLPLWVPIPPLAEVQVALEAAVAEVTQLQGYELKRIMRTGTVASTDNR
                     NWELLPSHHAPTSPERRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGE
                     IKLPGMANHFYRERVDQHLRIGIRALELLRAQGIDQLHSRKLRSFAEVAFQ"
     gene            complement(4702..5535)
                     /gene="metF"
                     /locus_tag="PKV56_08620"
     CDS             complement(4702..5535)
                     /gene="metF"
                     /locus_tag="PKV56_08620"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOX67919.1"
                     /translation="MSKKLPLSLEFFPPKTPEGVVKLAAVRQQLYALAPDFCSVTYGA
                     GGGTQEGTLSAVLAIRDEGVEAAPHFSCIGANAAGVREQLQQFRDAGLRRIVALRGDL
                     PSGYGRSGEFRYASDLVAFIRQETGNHFHIEVAAYPEMHPQAASPQADLKAYAAKVKA
                     GANSAITQMFFNTDAYFRFVDEAHALGADIPIVPGIMPITNASGIMRFADGCGTEIPR
                     WMRLRLLGFGDDSESIRAFGLDVVTDLCDQLRTAGVPGLHFYTMNQSAAVLEICRRLG
                     L"
     gene            complement(5552..6325)
                     /locus_tag="PKV56_08625"
     CDS             complement(5552..6325)
                     /locus_tag="PKV56_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOX67920.1"
                     /translation="MRADQLLLQRGLVPTRSAAQRLIDAGAVRWLGPKGWAVPRKAGE
                     DLPDGCSIEVTDNAELRFVSRGGLKLEAALDRCGFSVQGLHCLDAGQSTGGFTDLLLQ
                     RGAADVVGIEVGHGQLHARLAADPRVECHEGVNARDVGGSAFAQAHAEGSFDVVTGDL
                     SFISLTLVLPTLRRYLKPDGHLLMLVKPQFELQPEHIGKGGIVKDRRSFVEVETRIRA
                     ACREQALTVRDYFQCPIAGGGHGVTAGNTEFFVWAQPAP"
     gene            complement(6393..7820)
                     /gene="ahcY"
                     /locus_tag="PKV56_08630"
     CDS             complement(6393..7820)
                     /gene="ahcY"
                     /locus_tag="PKV56_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOX67921.1"
                     /translation="MNAVLKPNADYAVADLSQADFGRKEIKIAETEMPGLMAIREEFT
                     KSQPLKGARITGSIHMTIQTAVLVETLEALGAKVRWASCNIFSTQDHAAAALVANGTP
                     VFAYKGETLTDYWDYTHRIFEFGAKGSEGEGPNMILDDGGDATLLMHLGQRAEKDASV
                     LNNPTSEEETCLYAAIKAKLATDPTWYTRKSAQIIGVTEETTTGVHRLNEMSAKGTLL
                     FRAINVNDSVTKSKFDNLYGCRESLVDSIKRATDVMIAGKVAVVAGYGDVGKGSAQAL
                     RALSAQVWVTEIDPINALQAAMEGYKVVTMDYAADKADIFVSATGNKRVITHAHMVAM
                     KDEAIVCNIGHFDNEIDIASIEKYEWDEIKPQVDHVKFPDGKKIILLAKGRLVNLGCA
                     TGHPSFVMSASFANQTIAQIELFTKSAQYENGKVYVLPKHLDEKVARLHLKKVGAMLT
                     ELSDEQASYIGVSKTGPYKPDTYRY"
     regulatory      complement(7905..7979)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(8049..8675)
                     /locus_tag="PKV56_08635"
     CDS             complement(8049..8675)
                     /locus_tag="PKV56_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOX67922.1"
                     /translation="MTRAGLYVYSAAILALLTASGIGAFETGTWAMEVAPVFIVLPLM
                     LATHRRFPLTPLLYGLIALHMVVLMLGGHYTYARVPLGFWMEDVFGFTRNHYDRIGHL
                     MQGFVPAIAAREILLRRTTLAAPGMRGGWLFLVVTSICLAFSAIYELIEWGVAEAMGA
                     ASEDFLGTQGDPFDTQKDMFCAWLGAMAAQWLLARWHERQLAGLPAAR"
     gene            complement(8689..9873)
                     /gene="metK"
                     /locus_tag="PKV56_08640"
     CDS             complement(8689..9873)
                     /gene="metK"
                     /locus_tag="PKV56_08640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOX67923.1"
                     /translation="MANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPLSRVAAE
                     TLTNTGLVVLAGEITTQANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSN
                     DIAQGVDRASDDYLNLGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
                     LPFLRPDAKSQVTLRYVDGKPHSIDTVVLSTQHSPDQSETATKMKASFIEAVIEEIIK
                     PVLPKEWLKETRFLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDP
                     TKVDRSAAYACRYVAKNIVAAGLAKQCQIQVAYAIGVAKPMNITVYTEGTGVMSDDKL
                     SALVAEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWERTDKAQALRAAAG
                     LK"
     gene            10050..10976
                     /locus_tag="PKV56_08645"
     CDS             10050..10976
                     /locus_tag="PKV56_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX67924.1"
                     /translation="MTRFIAIFLTGCVRFMAHFPLRWLHAIGAPVGWLVYWASPSYRR
                     RFRANVAQAGVPWAQARDAIAASGRMMMELPPLWLRPPDEPLPVRVQWQGGELIEAAQ
                     AAGRGLVMLTPHLGCFEAIGQSYAQRYGAGRGAMTVLYRPARKPWLRDLVEAARRRPG
                     LDAAPATLAGVRQMIRALRRGEVVGLLPDQVPPEGLGVWAPFFGKPAYTMTLAARLVQ
                     QTGAALLLVWGERLPRGEGYCVRVSELGDALPADAADAAAVINRAMEGLILQCPQQYL
                     WGYQRYKRPRRGALGSSAGAPPAPPAGTGASS"
     gene            10973..11869
                     /locus_tag="PKV56_08650"
     CDS             10973..11869
                     /locus_tag="PKV56_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HOX67925.1"
                     /translation="MKLALLHLITRIVLALLWLLHWLPLGLLAPLGRGLGVVLWTLGR
                     ARRRIALRNLELCFPEKSGAEREAIALEHFKYLGRSLLERGLLWHASAERLKRLIHIE
                     GPADFGDRHAGALMWLVPHFVGLDVAAVAARFSVARPGCTVYQTQSNRLFDAAVRRGR
                     LRFGKIRVFSRHETARPLLRAIKEGDGFFNLPDMDFGRKDAEFVPFFGVPACTLLTPS
                     RMARLLHMTVQPVVVEMLPGGQGYRVRFLEPWTSFPTEDALADTAALNRWIESEIRRN
                     PAQYLWVHKRFKTRPRGEKSLY"
     gene            11919..12815
                     /gene="dapF"
                     /locus_tag="PKV56_08655"
     CDS             11919..12815
                     /gene="dapF"
                     /locus_tag="PKV56_08655"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOX67926.1"
                     /translation="MKIRFTKMQGAGNDFVVLDATQEPLALSTEQLRHLGDRRFGVGA
                     DQILVVARSGTPGVDFGYRIFNHSGEEVEHCGNGARCFVRYVRDKGLSDKSTLRVETV
                     NNLLELHLQPDGRVRVDMNAPVFDLPQIPFHAAGLRPHRVAVKGAAGPGRGGTIDMWP
                     LNLKGRTVEVAVLSMGNPHAVQVVDDVDTAPVEVQGPLIEHHTSFPKRVNAGFMQIVS
                     RSHIRLRVYERSAGETLACGTGACAAVVAGIRLGLLDAVVDVDARGGRLTIEWKCHEE
                     GPGSHVFMTGPAETVFEGEIEL"
     gene            12865..13554
                     /locus_tag="PKV56_08660"
     CDS             12865..13554
                     /locus_tag="PKV56_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOX67927.1"
                     /translation="MSSPTGVQGITEADIANYLANTPGFFERHAELLSSIQLLSPHGN
                     RAVSLQERQMDMLREKHKGLEQKIMEMIRHGQENVAIADRLHRYTRALMLTAEAGELP
                     DVLVRELMHQFLIPQAGIRVWGAASEHAALPFAREVSADAKSFASSLTLPYCGANSGF
                     EAAAWLEDAATVASLALIPLRHGANSEAFGLLVLGSPDPTRFSADMGTEFLMRIGEIA
                     SASLSRLIRPE"
     gene            13551..14519
                     /locus_tag="PKV56_08665"
     CDS             13551..14519
                     /locus_tag="PKV56_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOX67928.1"
                     /translation="MTRPAGAEAEQPALHDDLVRHLQHLQVERRLAERTRVMYAQAFE
                     RLHRFADAAAVPLREVQPHHVRRWAGQLHAQGLAPGSIAIQLSAWRGFYRWLGRDGLV
                     VQNPVAGVRAPRAAKPLPKALSVDQAVALADFDEDTEARQDSAALAARDQCMTELLYG
                     CGLRVSELVALDAQPGPQAVGWIDVADGTAHVLGKGQKRRSVPVGGPALRALQDWLSL
                     RVQMNRGGAEAALFLSNRGTRITTGQVRSRLKLRALKAGLPTHVHPHMLRHSFASHLL
                     QSSGDLRAVQELLGHANITTTQVYTKLDFGHLSKVYDAAHPRARRK"
     gene            14516..15715
                     /locus_tag="PKV56_08670"
     CDS             14516..15715
                     /locus_tag="PKV56_08670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX67929.1"
                     /translation="MKVIKLREGKERSLLKRHPWVFEGSIGSGRADSGETVRVESNAG
                     EFLAWSSGSPNSSIRVRAWSFNAAERIDAAFFERRIARAAAVRARLPIASNAMRLVHG
                     EADGLPGLVVDRYGDTLSAQFTAAGVERWKPVIADALLKVTGLTRLYERSDASVRELE
                     GLPPITGWLRLPPGADDSTEVTIQEHGWRLTVDVARGHKTGYYLDQRDSRKLFADCVA
                     HFRLQRVLNCYSYTGGFSVAALAGGAAEVTSVDSSGPALERAAAHVALNGFDAGRHTA
                     IDADVNATLRRFIDEGRRFDAIVLDPPKFASTAGQAERAARAYKDINRLAFMLLEPGG
                     LLFTFSCSGGVSADLFHKIVAGAGLDAGIDGQVHARTGAAPDHPMTVTFPEGEYLKGL
                     VIVKQIA"
     gene            15826..17721
                     /gene="glmS"
                     /locus_tag="PKV56_08675"
     CDS             15826..17721
                     /gene="glmS"
                     /locus_tag="PKV56_08675"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOX67930.1"
                     /translation="MCGIVGAVSTRNIVPVLIEGLKRLEYRGYDSCGVAVQQHGGLRR
                     ARSTERVAELELNVSADHIEGATGIAHTRWATHGAPAVHNAHPHFSPGPSAVGAVEGG
                     AADGVAAVGRVALVHNGIIENHDELRAELRGKGYVFHSQTDTEVLAHLIDHLYQGDLL
                     DAVQRTVPLLRGAYAIAVFCRDEPGRVIGARAGSPLILGVGTGERAGDHYLASDAMAL
                     AGVTDQIVYLEEGDVVDLRLGSVAISSAADGRFHAVQRHVRTVQAHSGAAELGPYRHY
                     MQKEIFEQPKAIADTLDAVTSISSDLFGDAADRVFKDIDSVLILACGTSYYAGSVAKY
                     WLEGIAKIPTSVEVASEYRYRDSVPNARTLVVTISQSGETADTLAALKHARSLGMLHT
                     LTVCNVSTSAMVRECEQAFITRAGVEIGVASTKAFTTQLVGLFLLTLAIAQTRGHLSD
                     DAQAQHLHALRHLPVAVQGVLALEPQVIAWAEVFARKHNALFLGRGMHYPIALEGALK
                     LKEISYIHAEAYPAGELKHGPLALVTAEMPVVTVAPNDALLEKLKSNLQEVRARGGEL
                     FVFADADTRIESGEGLHVIHMPENYGPLSPILHVVPLQLLAYHTACARGTDVDKPRNL
                     AKSVTVE"
     gene            18258..18794
                     /locus_tag="PKV56_08680"
     CDS             18258..18794
                     /locus_tag="PKV56_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67931.1"
                     /translation="MGLLASCSAWAVTVSSKSVSMPSGESRTVKIAKVSGTLVVTNSS
                     PAVVGVTRVDASTYRISGSAAGKAEVKFKDARSDVKVKVAVTAAAASTPPPPTAALPT
                     GALNGRLLASNCFQCHGTNGTGGFDRLAGKSASEIYGELREFASGKEDANGIMAAHAM
                     GFSDAQLKSIASYFASVR"
     gene            18804..20108
                     /locus_tag="PKV56_08685"
     CDS             18804..20108
                     /locus_tag="PKV56_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX67932.1"
                     /translation="MKRRQFIQSTAGAGVTFAAGLLPFAARAASLRVVVVGGGMAGAA
                     VAKFIRLWSKGEISVSLIEKDTSYVSNILSNEVVTGKRPSIDSLAYSYTALTTKYGVQ
                     RIQGTVTGIDDINKTVTYETPAGSNSVLTYDRLVLAPGLDFDLMPGMTSASQYDTTVP
                     HAWKAGVQTTLLRNQLLAMPDWQNVVITVPLAPYRCPPGPYERACVIADWLRTRKPNS
                     KVVMLDANPEIQAQKTNFQNAFNGAYGYRVDYRFGCTVNQLQLVAGAHNVTYTRNGIT
                     ESVAAAVLNPIPPQRAPGLLARSGLLNGKFAPVNLLTYESTLKAGIHVIGDACISGMP
                     KAGHIGNQEAKTCANAIVNALKGSLYALEETSPVLNSACYTPLTSTRATWLSAVYQYT
                     NGVMVPSTENGVTQPRAATSATTGNYEDMQKWFTTLMGDTFA"
     gene            complement(20473..21396)
                     /locus_tag="PKV56_08690"
     CDS             complement(20473..21396)
                     /locus_tag="PKV56_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67933.1"
                     /translation="MELPDSGALPRWTDLRDRYRQALTSASYLGLLAFGLRLGQPSAW
                     RACLALIAGVGLLAWASSYKRARAIADVATSRIGSAAQGYVEIVGRASTTPSELIVSP
                     LSGIACIWYRYRVYSKDGNRGEWREIDSGTSATTFEIADGTGACRVDPEHAEVVSPER
                     RVTYRDGDKLVEELLFAGSPIYVLGAFCTVHGPQTASSLSEDVGALLATWKRDPVELH
                     RRFDLDGNGEIDLREWEVARRLATRTVEKQHRDIRDLGEVHLMRAPSDSRLYLISALP
                     PHRIRRTFLWWSLFHLAVALVGAVALLGQWR"
     gene            complement(21402..21968)
                     /locus_tag="PKV56_08695"
     CDS             complement(21402..21968)
                     /locus_tag="PKV56_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOX67934.1"
                     /translation="MATILWISLPILLVYGVILFNSLVSVKNAVSKAWANIDVLLKQR
                     HDELPKLVDTCRQYMQHEQLTLEKVIQARARVADARESHDIGALGLAEGALRSGLGNL
                     FALAESYPDLKANEQFLHLQSRISGLESAIADRREFYNESVNINNVSIEQFPELLIAR
                     LFKFKSFELLKFSADELKDVDIGQRFRA"
     gene            22150..>23623
                     /locus_tag="PKV56_08700"
     CDS             22150..>23623
                     /locus_tag="PKV56_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX67935.1"
                     /translation="MPTRPTRLLKLQLLAVFIALNTVAALWVASTLSEDRDYRVQKAE
                     STTQNLALVLDQNITTAVEKIDLALLSLVDEFERQFREGGRLDAVRANELVLTYTKRL
                     EGLNGIRIADAEGTVVLGTDVVPQARVSWADRPFFPKMRDHPDGGLLITPPLLGRVSG
                     VWMISFNRRINRPDGTFGGIVSASVPLEHLRALVAMPNLGSHGVVALRDADFGLIVRH
                     PALPTLPSGVVGSRNLPPELATLVAAGQTRATYHTQHTADGVERILSYRRIERGRFSL
                     LVGMGSEDYLAEWHASVRDSVLRLALFAVWSGLGCWLLWNSTRQLRQANERSQALLRG
                     ASDGIHVLDEQGLVVEASDAFARMLGRRLEDVIGMHLSQWDAHPETGGMLPQLKLMQE
                     HGEQALLETRLRRADGSLLDVEIAAYAISLDDRPMLFASARDITERKQSELAIRELNA
                     DLERRVRQRTAELEIVNAGLLQACDAAQAANRAKSSFLANM"
BASE COUNT         3959 a         7747 c         7773 g         4144 t
ORIGIN      
        1 cggaagaagg gtgagatacc catcatcacc agggtcgaag tcactgtctc gggtgcggtg
       61 accaatcgcg ctgagcgatt cttggcggcg aacgccatga ctttcagtgt catcaacggg
      121 tcgccggaga tcatttctcc aatcaggttc gcatcgacgt catcttcgtt ctgacgcatc
      181 gcctccagcg ccgctgcggt ttcggctcgc accggaatct cggcatcgcg gaagcaggct
      241 acccacgcat ccagatcagc caaaggctgc ttcaaacgca gcaagggttg tgtcaggtct
      301 gcactcggca tggtcacctc atcgccgaat tcgttcggtg gcatctgtct ggcctcgtta
      361 tcggccgcgg tgagcagccc tgaagtgaac ggcactgccg gcgtcgcagc acagcgtgct
      421 tcatcagcct catctcgact caacgctggc cgagcttgag tttcacgtcc agaccgacct
      481 tcctgttgac gaactcgaac gtcgcgacgc ggcccagatc aacatcaccc gatttgtaca
      541 ggcccgcctt gaccatctgc tggtagaaat ccagtatgcg ggccggattc atcgcaccga
      601 tgccctgcgt cagtgcgtcg cccgaatcga cgatgccctg ctgtttcatc agagccaccg
      661 aagattccag ctcgtccgca gtcatttcgg ggttgtcgcg gagcatcagt gcgtgggcgg
      721 tcttgcggtc gccgtacagg tagttgacac agccgatgat cgacgcatcg acgaagcgct
      781 gcaccacatc aggctgcctc gtgacggtgt ccgtgcgcgc ctctatcact gtcgaatagg
      841 tgctgaaccc gtggtcggcc agcagatgaa ccacgggttt gaatcgcccc tgtttctcga
      901 cgtagatcgg ctcggccacc gaatagccct gttgtatcgc cttcgggttg gtgaggaacg
      961 gaccgaggtt gtagttgtag ggcctcagcg cttcatcacg gaacccgtgc gtcgccttca
     1021 gccactgcca ccagctgaac tgagcatcct tcgcgatcag agcgagcggg gcggacttca
     1081 gcgcctcgaa tttctcgtaa ccctgccctg ggtgtgcgat cagcgactgc ggatccttct
     1141 ggaacaggcc ggccacgacg accgtcggaa ctccgttctt gacgttgtcg aagctgtgca
     1201 gcaggtttcc tgtcagcagg aagtcgatgc gtctggcggc cagcaaaggt cggttgttca
     1261 cctgagggcc gccctgaagc aaggtcacgt ccagtccgaa gcgcttgtac gtgccgtccg
     1321 cgatggcctg gtagaagccg ccgtgggcag cctgggcctt ccagttggtg gcgaagcgga
     1381 ccttgtcttc agctttcgct gttccgatcc gcgacaggag cagcgccacc agcactccac
     1441 tcacgattcg acctgttcga cgaagcatgt ccatcatctc gaattccctg aaagcttttc
     1501 ggatgcatgc cagcgaacca gccatcggcg cgacagtgca gtggacgcgg catggatcat
     1561 caatccggtc atcaccagca acagcagtgc cgcgaacatc tttggtgtct cggtgcggaa
     1621 actagcttcg aggatgcgcg atgccagccc ggtctcgcgg ccggcagctc ctgcgaccat
     1681 ctcggccgtg accgcgccga tgagactgag accgccggag accttcacgc ctgcgacgaa
     1741 gtaaggcagc gcgctcggca tgagcagaag ccgcagacgc tgcagcgggc cagctccgta
     1801 cagccggaac aggtcacgca gcagtggatc ggcagcgcgc agacccatca tggtgttgga
     1861 aaggatcggg aagaaagcga cgatccaggc gcacagcagc aatgctgctg tggtgctctc
     1921 cacgtagatc agcatcagcg gcgcgatcgc gacgataggt gtcacctgca gcaccaccgc
     1981 cagcggcaac agcaccgctt cgagcggggc cgatagcgcg aacaccaccg cgatcagcac
     2041 gccgcccagg cacgccagca acagcgcacc gaaggtgatc ttcaacgtga accaccagct
     2101 catcgccagc gagccgaagt tctccatcag tgtttgcagc acgagactcg gcgcgggcaa
     2161 cgtgtagtgc gggatgcgtg caacgcgcac cagcacctcc cacgcgagca gccatcccag
     2221 cacgctcagc agcggcagcc atggggaatt cgtccagccc tgacggtgcc tggttgcctc
     2281 ggggtcgcgc tgcatcgtgc ggttcggggt cttgctcaag cgccgatccc atctgaagag
     2341 gaagcgctgg cccacgccaa tgcctgcgag atctcgcggc aggcttgcgc gtagcgcacg
     2401 gaggtccgga actctgtcgt ccgcggcacc ggctcgtcga tgaccacctc cgccgcgatc
     2461 cgtccgggtc gcgagcccat caccagcacc cgctggctca ggaaaacggc ctcgaagacg
     2521 ctgtgcgtta cgaacagcac cgcgagtgcg gttgattgcc accaagccag cagatcgtca
     2581 ttgagccgct gccggctgat ctcgtcgagc gcagcgaacg gctcgtccat cagcagcaag
     2641 cgtgggtgcg tgaccagtgc gcgcgcgatc gaggcccgca tgcgcatgcc gccggacagc
     2701 tcggcaggga atgcggcctc ggactcctcc agcccgaccg ctgccagcac ccgtcgcaca
     2761 tccggcgcag catcgctcct cgatcgaccg gccagacgca gcggcagcca gacgttgtcg
     2821 aacacgctgg cccaaggcat cagcgtcggg tcctggaaca cgaaggccgt atcaggcgcg
     2881 cgtcgggccg ctgcgctcga cagcgcaggc gacgtgatct gcccgtcgct gtgcacgtcg
     2941 agtccggcga cgaggcgaag caccgtgctc ttgccgcagc ccgaaggccc cagcagggaa
     3001 acgaactcgc gctcgccgac ttcgaacgac acgccatgca gtgccagcgt gccgttcgcg
     3061 tatcgcttgc cgacgtcgcg cagcacgatc gacggcgcag tcgagcctgc tgcggcggct
     3121 gccatcagcg aagcgacccg ctcactggaa cgccacctcg gcaaagcttc gcagcttgcg
     3181 gctgtgcaac tggtcgatgc cctgtgctcg cagcagttcg agtgcgcgga tgcctatgcg
     3241 caggtgctga tcgacccgct cgcggtagaa gtggttggcc atgccgggca gcttgatctc
     3301 gccatgcagt ggcttgtcgc tgacgcagag caaggtgccg tagggcacac gaaagcggaa
     3361 tccattggcc gcgatcgtgg cgctttccat gtcgagcgcg atcgctcgac tctggctgaa
     3421 gcgccgctcg ggcgatgtcg gagcgtggtg cgacggcagc agttcccagt tgcggttgtc
     3481 ggtgctggcc accgtgccgg tgcgcatgat gcgcttgagc tcgtagccct gcagctgcgt
     3541 cacctccgcc acggccgcct ccagtgcaac ctgcacctcg gccagcggcg ggatcggcac
     3601 ccacagcggc aggtcctcgt cgaggacgtg gtcctcgcgg acatagccat gcgccagcac
     3661 gtaatcgccg agctgctgcg tgttgcgcag cccggcgcag tggccgagca tgagccacac
     3721 gtgagggcgc agcaccgcga tgtggtcggt gatggtcttc gcgttggccg gtccgacgcc
     3781 gatgttgacc atcgtgatgc cggcgccgtc gtcgcgccgc aggtggtacg ccggcatctg
     3841 gggcagacgc ggcggcggtg cgccgagttc gtctccttgc tgcacgttca gcccccgacg
     3901 tcgcgtgatc actcccccgg gttcgacgaa ggcgcagtat tcgctcgccg ggtcgctcat
     3961 cgctgtgtgg cccaaccgga cgaactcgtc gatatagaac tggtagttgg tgaacagcac
     4021 gaagttctgg aaatgctccg gagccgtgcc ggtgtagtgg cgcaggcggt gcagcgagta
     4081 gtcgacacgc ggcgcggtga acagggccag cggttggcgg atcttgccgt ccttgccggg
     4141 ctggggttca tgcgttccgt tggcgatgcc atcgtccatc tcgcccaggt ccggcaggtc
     4201 gaacagatcc cgcatcagga gtcggcgtgc cgggtcgagc gaaccctcga ggtgctcgtg
     4261 ttcggccagc gagaagtgcg ccggtatcgg ctgagagctg gtgccgatct ccagcgacac
     4321 ctggtggttg cgcagcagca gatcgaattg cagctggtag tagtttccga acaggtccgg
     4381 ccgcgtcagc gtggtctcgt aggtgccggg gccggcgaca aagccataac tgagccgcga
     4441 gtcggctcgc gcgacggtgt cggtgtgcac cctgacgaaa gggtagcagg cacgcacccg
     4501 ggccggcagt gtctcgccac cgacgaaccg gtgcagcgaa gcgcgaagat gcgccacgct
     4561 ggtttcgtag atggtgcgaa cctgcgcgag cgccgctgca gggtcatcga agcggctcgg
     4621 ggcgatgtag gtcggtgcaa tcggcatggg cgctctttcg gcaacagccc ccattctccg
     4681 cggctcggtg aagacttgcg gtcacaaccc caggcgtcga cagatttcca gcaccgcggc
     4741 cgactggttc atcgtgtaga agtgcaggcc cggcacaccg gcggttcgca actggtcgca
     4801 caggtcggtg acgacatcca ggccgaacgc gcggatcgat tcgctgtcat cgccgaaacc
     4861 cagcagcctc aaccgcatcc agcgcggtat ctcggtgccg cagccgtcgg cgaaccgcat
     4921 gatgccgctg gcgttggtga tcggcatgat gccgggcacg atgggtatgt ctgctccgag
     4981 cgcatgcgct tcatcgacga agcggaagta cgcatcggtg ttgaagaaca tctgcgtgat
     5041 cgcgctgttg gcgccggcct tgaccttggc ggcataggct ttcagatcgg cttgcggcga
     5101 ggctgcctgc gggtgcatct ccggatacgc cgcgacctcg atgtggaagt gattgccggt
     5161 ttcctgccgg atgaacgcga ccagatcgct cgcgtagcgg aattcgccgg aacgtccgta
     5221 gccgctgggc agatcgccgc gcaacgcgac gatgcggcgc agaccggcgt ccctgaactg
     5281 ctggagctgt tcgcgtaccc cggcagcatt ggcgccgatg cacgagaagt gcggcgccgc
     5341 ctcgacgccc tcgtcacgga tcgcgagcac cgccgacagc gtgccttcct gcgtgccgcc
     5401 accggcaccg taagtcacgg aacagaagtc cggcgccagc gcgtacagct gctgccggac
     5461 cgccgccagc ttgaccacgc cttcgggggt cttgggcgga aagaactcga ggctcagcgg
     5521 aagtttcttg ctcatgctgt tctggggggt gtcatggggc cggttgggcc cacacgaaga
     5581 attcggtgtt gcccgcggtg acgccgtgtc cgcctccggc gatcgggcac tggaagtagt
     5641 cgcgcactgt cagcgcctgc tcgcggcacg ccgcgcggat acgggtttcg acctcgacga
     5701 agctgcgccg gtccttgacg atgccgccct tgccgatgtg ctcgggctgc agctcgaact
     5761 gcggcttcac cagcatcagc aggtgaccgt ccggcttcag atagcgcctc agcgtcggca
     5821 gcaccagcgt cagcgaaatg aacgacaggt caccggtgac gacatcgaac gagccttcgg
     5881 catgcgcctg ggcgaaggcg ctgccgccga catcgcgcgc gttgacgcct tcatggcatt
     5941 cgacgcgcgg gtcggctgcc agccgggcgt gcagttgccc gtgaccgacc tcgatgccga
     6001 cgacatccgc cgcgccgcgc tgcagcagca ggtcggtgaa gccaccggta ctctggccgg
     6061 catcgaggca gtgcagcccc tggacgctga agccacaacg atccagtgcg gcttccagct
     6121 tcaagccgcc gcgtgacacg aagcgcaact cggcgttgtc ggtcacttcg atcgaacagc
     6181 cgtccggcag gtcttcgccc gccttgcgcg gcaccgccca acccttggga ccgagccagc
     6241 gcacggcgcc tgcatcgatc agccgttgcg cggccgatcg ggtaggcacg aggcctcgtt
     6301 gtaagagcaa ctgatcagcg cgcatggata aggatcaatc tactcgccgc ggaacgggtt
     6361 tcccggccgc gggcgaggcg ccccctgggg gctcaatacc tgtaggtgtc gggcttgtag
     6421 gggccggtct tcgacacgcc gatgtagctg gcctgctcgt ccgacaactc agtcagcatc
     6481 gcgccgacct tcttcaggtg cagtcgcgcc accttctcgt cgaggtgctt gggcagcacg
     6541 tagaccttgc cgttctcgta ctgggcgctc ttggtgaaga gctcgatctg cgcgatggtc
     6601 tggttggcga agcttgccga catcacgaag ctggggtggc cggtggcgca gcccagattc
     6661 accaggcggc ccttggccag caggatgatc ttcttgccgt ccgggaactt gacgtggtcg
     6721 acctgcggct tgatctcgtc ccactcgtac ttctcgatcg aggcgatgtc gatctcgttg
     6781 tcgaagtggc cgatgttgca gacgatggcc tcgtccttca ttgccaccat gtgcgcgtgc
     6841 gtgatgaccc gcttgttgcc ggtggcgctg acgaagatgt ccgccttgtc ggcagcgtag
     6901 tccatcgtca cgaccttgta gccttccatc gcggcctgca gcgcgttgat cggatcgatt
     6961 tcggtcaccc agacctgggc gctcaaggct cgcagggcct gggcgctgcc cttgcccaca
     7021 tcgccgtagc cagcgacgac ggcaaccttg ccggcgatca tcacgtcggt ggcgcgcttg
     7081 atcgaatcga ccagcgattc gcggcagccg tacaggttgt cgaacttgct cttggtgacg
     7141 ctgtcgttga cgttgatcgc ccggaacagc aacgtgccct tggcgctcat ctcgttgagt
     7201 cggtgcacgc cggtcgtcgt ctcttcggtc acgccgatga tctgtgccga cttgcgggtg
     7261 taccaggtcg gatcggtcgc gagcttggcc ttgatcgctg cgtagaggca ggtttcctct
     7321 tcgctggtcg ggttgttcag caccgaggcg tccttctcgg cgcgttggcc caggtgcatc
     7381 agcagcgtcg cgtcgccgcc gtcgtcgagg atcatgttcg ggccttcgcc ttcggacccc
     7441 ttggcgccga actcgaagat gcggtgcgtg tagtcccagt agtcggtcag cgtctcgccc
     7501 ttgtaggcga acaccggcgt tccgttggcg accagcgcgg cagccgcatg gtcctgcgtc
     7561 gagaagatgt tgcacgaggc ccagcgcacc ttcgcgccga gtgcttccag cgtttccacc
     7621 agcacggcag tctggatggt catgtggatg ctgccggtga tgcgtgcgcc cttgagcggc
     7681 tggctcttgg tgaactcttc gcggatcgcc atcagaccgg gcatttcggt ctcggcaatc
     7741 ttgatttcct tgcggccgaa atcggcttgc gacaggtcgg cgaccgcgta gtcggcgttg
     7801 ggtttcagaa cggcgttcat gtgtggctcc aaagaatgag gaacccgcac ggcttcgcag
     7861 ggaacggacg ctgaaattac cagctcaccc acgaagaacc atcgtgcggg tgagcgcggt
     7921 tgcaatgaag aactccgagc ctgggacaca tgacgtgcct tgcaacgctc ctcggaactg
     7981 ggagttattg taatcggcct cggacccgtc gacttgagcg cggccttgcc ggtgtggccg
     8041 cttccgtctc agcgagcggc cggcagccct gccagttgcc gctcgtgcca gcgcgccagc
     8101 agccactgcg ctgccatcgc gccgagccag gcgcagaaca tgtccttctg ggtgtcgaac
     8161 ggatcgccct gcgtgcccag gaaatcttcc gaggccgcgc ccatggcttc ggccacgccc
     8221 cactcgatca gttcatagat cgcactgaag gccaggcaga tcgatgtgac cacgagaaac
     8281 agccagccgc cgcgcatccc cggcgccgcc agcgtcgtgc gtcgcagcag gatctcgcgc
     8341 gcagcgatgg cgggtacgaa cccctgcatc aggtgcccga tccggtcgta gtggttgcgt
     8401 gtgaatccga acacatcctc catccagaag ccgagcggca cccgggcgta ggtgtaatgc
     8461 ccgccgagca tcagcaccac catgtgcagc gcgatcaggc cgtagagcag aggcgtcagc
     8521 ggaaagcgcc gatgcgtggc cagcatcagg ggcaggacga tgaagaccgg agcgacctcc
     8581 atcgcccagg ttccggtttc gaaggccccg atgcccgatg ccgtcagcag cgcgaggatc
     8641 gctgcgctgt agacgtacag ccctgcgcgg gtcatgacct gcccagggct acttcagccc
     8701 cgccgccgca cgcagcgcct gcgccttgtc cgtgcgttcc caggtgaact ccggctcttc
     8761 tcgtccgaag tgaccatagg ctgcagtctt ctcgtaaatc gggcgcagca ggtcgagcat
     8821 ctggatgatg cctttcggac gcaggtcgaa gtgctcggcc accagcgccg acagcttgtc
     8881 gtcggacatg acgccggtgc cttcggtgta gaccgtgatg ttcatcggct tggccacgcc
     8941 gatcgcataa gccacctgga tctggcactg cttggcgagt ccggcggcga cgatgttctt
     9001 cgcgacgtag cggcaggcgt aggccgccga gcggtcgacc ttggttggat ccttgccgct
     9061 gaacgcgccg ccaccgtgcg ggcaggcgcc gccgtaggtg tcgacgatga tcttgcggcc
     9121 ggtcagtccg cagtcgcctt gcggcccgcc gatcacgaag cggccggtcg gattgaccag
     9181 gaaccgggtc tccttcagcc attccttcgg gagcaccggc ttgatgattt cctcgatcac
     9241 cgcctcgatg aagctggcct tcatcttggt ggcggtttcg ctctggtcgg ggctgtgctg
     9301 cgtggacagc acgacggtgt ctatcgagtg cggcttgcca tcgacgtagc gcagcgtcac
     9361 ctggctcttc gcgtcgggac gcaggaaggg caggcgaccg tccttgcgca actgcgcctg
     9421 acgctccacg agacgatgcg cgtagtagat cggcgcgggc atcagctcgg gcgtctcgtc
     9481 gcacgcgtag ccgaacatca gtccctggtc gcccgcgccg aggttcaggt aatcgtcgct
     9541 cgcgcgatcg acgccctgtg cgatgtcgtt gctctgcttg tcgtaggcca ccagcaccgc
     9601 gcagcccttg tagtcgatgc cgtactcggt gttgtcgtag ccgatgcgct tgatggtgtc
     9661 gcgtgcgacc tggatgtagt cgacgttagc ctgcgtcgtg atctcgcctg ccagcaccac
     9721 gaggccggtg ttggtcaggg tctcggccgc tacacgcgac agcgggtcct gcttgaagat
     9781 cgcatcgagg atcgcgtcgg agatctggtc cgcgaccttg tcggggtgac cttcggagac
     9841 ggactcggat gtgaaaagga aatcgttcgc cactcttaaa ctccggttgg ttgcaacttg
     9901 cgttgccggg ccagcttctg gaggtgcggc gaacgcttta gcggtttgtt gagcctaagc
     9961 ccggcagcca cttctgatgc cccgaagtgc cttgcaatcg ccccgcaagt agttcattaa
    10021 ctcggcgatg gccggattct agcgatctga tgacccgctt cattgcgatt tttctcacag
    10081 gctgtgtgcg cttcatggcg catttcccgc tgcgctggct gcacgcgatc ggcgcgccgg
    10141 tggggtggct ggtgtactgg gcgtcgccca gctatcgacg gcgcttccgc gcgaatgtcg
    10201 cgcaggccgg cgtgccgtgg gcccaggctc gggacgccat cgccgcatcg gggcggatga
    10261 tgatggagct gccgccgctc tggctgcgtc cgccggatga gcccctgcca gtacgggtgc
    10321 agtggcaggg cggcgaactg atcgaggcgg cccaggctgc cggccggggc ctggtgatgc
    10381 tgacgccgca cctgggctgc ttcgaggcca tcggccagtc gtatgcccag cgctatggtg
    10441 ccgggcgtgg cgcaatgacc gtgctctatc gcccggcgcg caagccctgg ctgcgcgatc
    10501 tggtcgaggc cgcgcgccgt cgacccgggc tggacgccgc gcccgcgacg ctggctggcg
    10561 tgcgccagat gatccgtgcc ctcaggcgtg gcgaagtcgt cggcctgctg ccagaccagg
    10621 tgccgcccga aggcctgggc gtgtgggcac cgttcttcgg caaacctgcc tacacgatga
    10681 ccctcgccgc gcgactcgtc cagcagaccg gggcggcgct gcttctggtg tggggagagc
    10741 gcctgccccg tggcgaggga tattgcgtgc gcgtcagcga actgggcgac gcattgcctg
    10801 ccgacgctgc cgacgcggcg gccgtcatca accgcgcaat ggaagggctg atcctgcaat
    10861 gcccgcagca gtacctgtgg ggctaccagc gctacaagcg cccgcgccgc ggcgcgctgg
    10921 gatcgtccgc aggggccccg ccggcgcccc cggcgggcac aggggcatcg tcttgaagct
    10981 ggccttgctg cacctgatca cgcgcatcgt gctggcgctg ttgtggctac tgcactggct
    11041 gccgctcggg cttctggccc cgctggggcg tgggctgggg gtggttctct ggacgctggg
    11101 tcgcgcgcgc cgacgcatag cgttgcgcaa cctcgaactg tgctttcctg aaaagagcgg
    11161 ggcagagcgc gaggcgattg cactcgagca cttcaagtac ctgggccgca gcctgctgga
    11221 gcgcgggctg ttgtggcacg catccgccga gcgactcaag cggctgatcc acatcgaggg
    11281 ccctgccgat ttcggtgacc gtcatgccgg tgcgctgatg tggctggtgc cgcacttcgt
    11341 ggggctcgac gtggccgccg tcgctgcgcg gttctccgtt gcgcggcccg ggtgcacggt
    11401 ctatcagacg cagagcaaca ggctcttcga tgcggcggtg cggcgcggcc ggttgcgctt
    11461 cggcaagatc cgcgtgttct cgcgccacga aaccgccagg ccgctgctgc gtgcaatcaa
    11521 ggaaggcgat ggattcttca acctgcccga catggacttc ggccgcaagg atgccgagtt
    11581 cgtgccgttt ttcggcgtgc cggcgtgcac cctgctgacg ccgtctcgca tggcgcgact
    11641 gctccacatg acggtgcagc cggtggtggt cgaaatgctg cccggtggcc agggctaccg
    11701 ggtgcgcttc ctcgagccct ggacgagttt cccgaccgaa gatgcgttgg ccgacaccgc
    11761 cgccctgaac cgctggatcg agtccgagat ccgccgcaat cccgcgcagt acctgtgggt
    11821 gcacaagcgc ttcaagacgc ggccgcgcgg cgagaaatcg ctttactgac gtctgttctt
    11881 cgccggaatg gcccggcctg ggcaacgata atcacgccat gaaaatccgg ttcaccaaga
    11941 tgcagggcgc cggtaacgac ttcgtcgtgc tcgacgccac gcaggagccg ctcgcgctca
    12001 gcaccgagca gttgcgccac ctcggcgatc ggcggttcgg cgtgggcgca gaccagatcc
    12061 tggtggtcgc tcgcagcggc acgcccgggg tcgacttcgg ttaccgcatc ttcaatcaca
    12121 gcggcgaaga ggtcgagcat tgcggcaacg gcgcacgctg tttcgtgcgc tacgtgcgcg
    12181 acaagggtct gtccgacaag agcacgttgc gcgtcgagac tgtcaacaac ctgctcgagt
    12241 tgcatctgca gcccgatggc cgcgtgcgcg tcgacatgaa cgcaccggtg ttcgacttgc
    12301 cgcagatccc gttccatgcg gccggtcttc ggccgcaccg ggtggccgtc aagggcgctg
    12361 ccggcccggg caggggtgga accattgaca tgtggccact gaacctgaaa gggcgaaccg
    12421 tcgaggtggc ggtgctgtcg atgggcaatc cccatgcggt gcaggtggtc gacgacgtcg
    12481 acaccgcacc cgtcgaggtg cagggcccgc tgatagagca tcacaccagc tttccgaagc
    12541 gcgtcaacgc cggcttcatg cagatcgtct cgcggagtca catccggctg cgggtgtacg
    12601 agcgcagtgc cggcgagacg ctggcttgtg gcaccggtgc ctgcgccgct gtcgtggccg
    12661 gcatccggct cgggctgctg gatgcggtgg tcgatgtgga cgcccgtggc ggccgcctca
    12721 cgatcgaatg gaagtgccac gaagagggtc cgggcagcca tgtgttcatg accggaccgg
    12781 ctgagaccgt gttcgaagga gagatcgaac tgtgaatcac gaaaccgcta tgaaccgaaa
    12841 gcaacccacc atgagcacca agccgtgagc agccccaccg gagtccaagg catcaccgaa
    12901 gccgacatcg ccaactacct ggcgaacacg cccggcttct tcgagcgcca tgccgagctg
    12961 ctcagctcga tccagctcct cagtccgcat ggcaaccgcg ccgtctcgtt gcaggaacgt
    13021 cagatggaca tgctgcgcga gaagcacaag gggctcgagc agaagatcat ggagatgatc
    13081 cgccacggcc aggagaacgt cgccatcgcc gaccgtctcc accgctacac gcgcgcgctg
    13141 atgctgacag ccgaagcggg cgaactgccc gacgtgctgg tgcgcgagct gatgcatcaa
    13201 ttcctgattc cacaggccgg tatccgcgtc tggggcgcag cgtccgagca tgcagcgctg
    13261 ccgttcgccc gcgaagtcag cgccgatgcg aagagcttcg cctccagcct gacgctgccc
    13321 tactgcggcg cgaattccgg cttcgaggca gcggcctggc tcgaggatgc cgctaccgtg
    13381 gcatcgctgg cgctgattcc gctgcgtcat ggcgccaaca gcgaagcttt cgggctgctc
    13441 gtgctcggtt cgcccgatcc gacgcggttt tcggccgaca tggggaccga gttcctgatg
    13501 cgcatcggcg agattgccag cgccagcctg tcacgcctca ttcgaccgga atgacccgac
    13561 ccgccggcgc cgaagccgag cagccggctc tgcacgacga cctggtgcgt cacctgcagc
    13621 atctgcaggt cgagcgtcgg cttgccgaac gcacccgtgt gatgtacgcg caggcgttcg
    13681 agcggctgca ccgctttgcc gatgcggccg cagtgccgct gcgcgaggtg cagccgcatc
    13741 acgtgcgacg ctgggccggc cagctgcacg cgcaggggct ggcgcccggc agcatcgcga
    13801 tccagctgtc ggcgtggcgc gggttctatc gctggctggg ccgcgacgga ctcgttgtgc
    13861 agaacccggt ggcgggcgtg cgggccccgc gggcagccaa gccgttgccg aaggcgctgt
    13921 cggtcgacca ggcggtggcg ctggccgatt tcgacgagga caccgaggcg cgtcaggaca
    13981 gcgcggcact cgctgcccgc gatcagtgca tgaccgagct gctgtacggc tgcggcctgc
    14041 gggtcagcga gctggtggcg ctcgatgcgc agcctggccc gcaggccgtc ggctggattg
    14101 acgtcgcgga cggtaccgcg cacgtgctcg gcaagggcca gaagcgacgc agcgtgccgg
    14161 tcggtggacc ggcgttgcgt gcgttgcagg actggctgtc gttgcgggtc cagatgaacc
    14221 ggggcggcgc ggaggccgcg ctgttcctga gcaaccgcgg cacccgcatc accaccggtc
    14281 aggtgcgatc gcgcctgaag ctgcgtgcat tgaaggccgg cctgcccacg cacgtgcatc
    14341 cccacatgtt gcgccactcg ttcgcgtcgc acctgctgca gtccagcggc gacctgcgcg
    14401 ccgtgcagga gctgctgggg cacgccaaca tcacgacgac gcaggtctac accaagctcg
    14461 acttcggcca cctgagcaag gtgtacgacg cggcgcaccc cagagccagg cgcaaatgaa
    14521 ggtcatcaag ctgcgcgagg gcaaggagcg ctcgctgctc aagcggcatc cgtgggtgtt
    14581 cgagggcagc atcggcagcg gccgcgccga ttccggtgaa acggtgcgcg tcgagtcgaa
    14641 tgcgggcgag tttctcgcct ggtcttccgg cagcccgaac tcgtcgatcc gcgtgcgggc
    14701 ctggagcttc aacgcggccg aacgcatcga cgctgccttc ttcgagcgcc gcatcgcccg
    14761 cgccgccgcg gtgcgtgcgc gcctgccgat cgcgagcaac gccatgagac tggttcatgg
    14821 cgaggccgat ggcctgcccg gactggtcgt cgatcgctac ggcgacacgc tcagcgcgca
    14881 gttcaccgcc gccggggtcg agcgctggaa gccggtgatc gccgacgcgc tgctgaaagt
    14941 cacggggttg acgcgcctct acgagcgaag cgacgccagc gtgcgcgaac tggaaggact
    15001 gccccccatc acgggctggc tgcgcctgcc gcccggtgct gacgacagca cggaagtgac
    15061 gatccaggag cacggctggc gcctgaccgt agacgtggcg cgcgggcaca agaccggcta
    15121 ctacctcgac cagcgcgaca gccgcaagct gtttgccgac tgcgtggcgc atttccgcct
    15181 gcagcgggtg ctcaactgct acagctacac cggcggtttc agcgtcgcgg cgctggccgg
    15241 cggggcggcc gaggtgacca gcgtcgattc ctcgggaccg gcgctcgagc gcgctgccgc
    15301 acatgtcgcg ctgaacggtt tcgatgccgg gcgacacacg gcgatcgatg ccgacgtcaa
    15361 cgcgacgctg cgccgattca tcgacgaagg acggcgcttc gatgccatcg tgctcgaccc
    15421 gccgaaattc gcctccacgg ccgggcaggc cgagcgagcc gcgcgcgcct acaaggacat
    15481 caatcgcctg gcgttcatgc tgctggaacc cggcggcctg ctgttcacct tctcgtgttc
    15541 gggcggcgtc agtgccgacc tgttccacaa gatcgtcgcc ggcgccgggc tcgatgccgg
    15601 catcgacggg caggtccacg cgcgcactgg tgcggccccc gatcacccga tgacggtgac
    15661 cttccccgag ggcgaatacc tcaagggttt ggtgatcgtc aaacagatcg cgtgattaat
    15721 gccgcatttg aatctttccg ccgcttcagg ctgagccgaa gcggcccgaa tccctggctg
    15781 gctgcgtagc atccgcgcag ttcaagagaa aacgggagat tttccatgtg tggcatcgtc
    15841 ggcgccgtca gcacccgcaa catcgttccg gtgctgatcg aagggctcaa gcggctcgaa
    15901 taccggggct acgactcctg cggtgtcgcg gtgcagcagc atggcggact gcgccgtgcc
    15961 cgcagtaccg agcgtgtggc ggaactcgag ctcaacgtgt cggccgacca catcgaaggg
    16021 gccaccggca tcgctcacac gcgctgggcc acgcacggcg cgcctgcggt gcacaacgcc
    16081 catccgcatt tttcgcccgg gccgtcggcc gtcggggccg tggagggcgg agcggctgac
    16141 ggcgtcgctg ccgtgggtcg cgtggcgctg gtgcacaacg gcatcatcga gaaccacgac
    16201 gagttgcgcg ccgagttgcg cggcaagggt tacgtcttcc acagccagac cgacaccgag
    16261 gtcctggccc acctgatcga ccatctctat cagggtgacc tgctcgacgc ggtgcagcgc
    16321 acggtgccgt tgctgcgcgg tgcctatgcg atcgcggtgt tctgccgcga cgagccgggt
    16381 cgcgtgatcg gcgcgcgcgc cggttcgccg ctgatcctgg gtgtcggcac cggtgaacgc
    16441 gcaggggatc actacctcgc cagcgatgcg atggcgctgg ctggcgtgac cgaccagatc
    16501 gtctatctcg aggaaggtga cgtggtcgat ctgcggctgg gaagtgtcgc tatcagttcg
    16561 gccgccgacg ggcgcttcca cgcggtgcag cgccacgttc gaacagtgca ggcgcattcg
    16621 ggtgccgccg agctcggccc gtatcgccac tacatgcaga aggaaatctt cgagcagccc
    16681 aaggcgatcg ccgacacgct ggatgcggtg acatccatct cgtccgacct cttcggcgac
    16741 gccgccgatc gcgtgttcaa ggacatcgac tcggtgctga tcctggcgtg cggcaccagc
    16801 tattacgcgg gctcggtggc caagtactgg ctcgaaggca tcgcgaagat tccgacctcg
    16861 gtcgaagtgg ccagcgagta tcgctaccgc gacagcgtgc cgaacgcgcg caccctggtc
    16921 gtcacgatca gccagagcgg cgagaccgcc gacacgctgg cggcgctgaa gcacgcccga
    16981 tcgctcggca tgctgcacac gctgaccgtc tgcaacgtgt cgaccagcgc gatggttcgc
    17041 gaatgcgagc aggcattcat cacgcgggcc ggcgtcgaga tcggggtggc atcgaccaag
    17101 gccttcacga cgcagctggt cggcctcttc ctgctgaccc tggcgattgc gcagacacgc
    17161 gggcacctga gcgatgacgc acaggcgcag cacctgcatg cgttgcgcca cctgccggtc
    17221 gcggtgcagg gcgtgctcgc gctcgagccc caggtcatcg catgggccga ggttttcgca
    17281 cgcaagcaca acgcgctctt cctcgggcgc gggatgcact acccgatcgc cctggaaggg
    17341 gcgctcaagc tgaaggaaat cagctacatc catgccgagg cctatccggc gggagagctg
    17401 aagcacggcc cgctggcgct cgtcactgcc gagatgccgg tggtgacggt cgcacccaat
    17461 gacgccctgc ttgaaaagct gaagagcaac ctgcaggagg tgcgggcacg cggtggcgag
    17521 cttttcgtgt tcgccgatgc cgatacccgc attgaatcgg gcgaggggct gcatgtgatc
    17581 cacatgcccg agaactacgg cccgttgtca ccgatcctgc atgtcgttcc gctgcagctg
    17641 ctggcctatc acaccgcctg cgctcgcggg acagacgtcg acaagccccg caacctggca
    17701 aagtcggtca cggtcgagta ggtctcgtcc ggctcgatcc cgacgtgttc gctgggaaca
    17761 ggttctctcg ggaatccgcc gctgcgccag agctcggcaa ttgatctcgt gccgattcca
    17821 ctcggctcaa acctccgtat tcccctgatc ctgtcgctgc cccaccaggc aacgacttcg
    17881 atgtgatctg cgcgctcgtg cgtcccggtc acgcctgggt accgcccgct ggctgcgcct
    17941 gctcggcttg aaatgagccg cctggaaggg cgtgcgcttg gcatcgcgcg ggcaggctgg
    18001 ccctctcaca cccacacaca accctgaagc tcgacgtaag gcggtcttca ctttcacctg
    18061 tcaatctctc cggcatgcat ggagccctcg gttgatcacg gtgccgcctc cttccatgaa
    18121 acggccttgg ccgcaggctg caacagccgc atgccagcgg actggcaacg cgaagttccg
    18181 tcacacaaca aagtccattg atgaaggggt cttgaatgag ggtagtcaaa agcactgtca
    18241 gaccgtggct cgtcgccatg ggtctgctcg cctcgtgctc cgcctgggcg gtcacggtct
    18301 cgtccaagtc cgtctccatg ccgtccggcg agtcgcgaac ggtcaagatc gccaaggtgt
    18361 cgggcacctt ggtggtgacg aactccagcc ccgctgtcgt tggcgtgacc cgggttgacg
    18421 cctcgaccta ccgcatcagc ggcagcgcgg cgggcaaggc ggaagtcaag ttcaaggacg
    18481 ctaggtcgga cgtcaaggtc aaggtggccg tgacggccgc ggcggcctcg actcccccgc
    18541 cccccacggc cgcgctgccc acgggagcgc tgaacggccg cctgctggca agcaattgct
    18601 tccagtgcca cggcaccaac ggcaccggcg gattcgatcg cctggccggc aagagcgcct
    18661 ccgagatcta tggcgagctg cgcgaatttg ccagtggcaa ggaagacgcc aacggcatca
    18721 tggccgcgca cgccatgggc ttctccgatg cccaactgaa gtccatcgcc agttacttcg
    18781 ccagcgtgcg ttgagaggtt cccatgaagc gtcgtcaatt cattcaatcc acagcaggcg
    18841 cgggcgtgac cttcgctgcc ggtcttcttc cgtttgctgc ccgtgcggct tccctccggg
    18901 tggtcgtggt cggtggcggc atggccggcg cggccgtggc caagttcatc cgcctgtgga
    18961 gcaagggcga gatcagcgtg tccttgattg agaaggacac gtcgtacgtg tccaacatcc
    19021 tgagcaacga ggtcgtgacc ggcaaacgcc catccatcga ctcgctggct tactcgtaca
    19081 ccgcgctgac caccaagtac ggcgtccagc gcatccaggg cacggtcacg ggcatcgatg
    19141 acatcaacaa gacggtcacc tacgaaaccc ctgccggaag caattccgtc ctgacctacg
    19201 accgcctggt gctggcgccg ggcctggact tcgacctcat gcccggcatg accagcgcaa
    19261 gccagtacga caccaccgtg ccgcatgcct ggaaggccgg cgtccagacc acgctgctgc
    19321 gcaaccagtt gctggccatg cctgactggc agaacgtggt catcaccgtg ccgctggcgc
    19381 cgtatcgctg cccgcccggc ccctacgaac gtgcgtgcgt gattgcggac tggttgcgaa
    19441 cccgcaagcc caacagcaag gtggtgatgc tcgacgccaa tccggaaatc caggcccaga
    19501 agaccaactt ccagaacgcg ttcaacgggg cctacggcta ccgggtcgac taccgcttcg
    19561 gctgcacggt gaaccagctg cagctggtgg ccggcgccca caacgtcacc tacacgcgca
    19621 acggcatcac cgagtcggtc gctgccgcgg tgctcaaccc gataccgccg cagcgcgccc
    19681 cgggcctgct ggcccgctcg ggcctgctca acggcaagtt cgccccggtg aacctgctga
    19741 cctacgaatc gaccctcaag gccggcatcc acgtcatcgg cgacgcctgc atctcgggca
    19801 tgcccaaggc gggacacatc ggcaaccagg aggccaagac atgcgccaac gccatcgtga
    19861 atgcgctcaa gggcagcctg tatgcgctgg aagagacgtc accggtcctc aactccgcct
    19921 gctacacgcc cctgaccagc acccgggcca cgtggctgtc ggccgtctac cagtacacca
    19981 acggagtgat ggtgccgtcg acagagaacg gcgtcactca gcccagggcc gccaccagcg
    20041 ccaccacggg caactacgag gacatgcaga agtggttcac aaccctcatg ggtgacacct
    20101 tcgcctgagt cccggcgtcc ggccgggggt aaccagcggc tcacacccgc tcaggcagcc
    20161 cccggaggca tcgcgatcaa ccgacagggg aatcggaaac ccgcaattcg cccgtcctgc
    20221 ctggtggcgt ttgagtggcc acaggcgggc aaacctgaag tggctatcag gggcgatcgg
    20281 aaggcgggag gtggccgatc agggccgccc gccagaggca gcttcccggc tctcagattc
    20341 cacggagttc gtcggtgcag gtcactgcag ggggctggaa atcgcgcgaa tccgggtctg
    20401 tcgcagagcg cgacaaaccg tgtttccaga gatcaggcgc cgatcaccgc agggtcttgc
    20461 ggcccgtgtg ggtcagcgcc actgccccag cagagccacc gccccgacca gcgccactgc
    20521 caggtggaaa aggctccacc agagaaaggt ccgtcgaatc ctgtggggcg gcagggccga
    20581 gatcaggtac aggcgggagt cggacggtgc gcgcatcagg tgcacttcgc ccaggtcgcg
    20641 gatgtcccga tgctgcttct cgacggttcg tgtggccagt cgcctggcga cctcccactc
    20701 gcgcagatcg atttcaccgt tgccgtccag gtcgaagcgc cggtgcagtt ccaccgggtc
    20761 gcgcttccag gtggcgagca gcgcgccgac atcctcgctc aggctcgacg ccgtctgtgg
    20821 tccatggacc gtgcagaacg cccccagcac gtagatcggg ctgccggcga acagcagttc
    20881 ctcgaccagc ttgtcgccat cacggtaggt gacccgccgc tcgggtgaca ccacttccgc
    20941 atgttccggg tcgacacggc aggctccggt gccgtcggcg atctcgaagg tggttgcgct
    21001 ggtgccgctg tcgatctccc gccattcgcc gcggtttccg tccttcgagt agaccctgta
    21061 gcggtaccag atgcaggcga tgccgctgag cgggctgacg atgagttcac tcggcgtcgt
    21121 gctggcgcgc cccacgattt ccacgtagcc ctgagcggct gagccaatgc gcgaggtggc
    21181 cacatcggcg atcgcgcggg cccgcttgta gctcgaagcc caggccagca agccgacgcc
    21241 ggcgatcagt gccaggcaag ccctccaggc cgaaggctgc cccaggcgca gcccgaacgc
    21301 cagcagaccg agatagctgg ccgatgtcag cgcttggcga tagcgatcgc gcaggtcggt
    21361 ccagcggggg agggctcccg agtcagggag ttccatgggg gtcaggcgcg aaagcgctgg
    21421 ccgatgtcga cgtccttcag ctcgtcggcc gagaacttca gaagttcgaa ggacttgaac
    21481 ttgaacaacc gggcgatcag cagttcgggg aactgctcga tgctgacgtt gttgatgttc
    21541 acgctctcgt tgtagaactc gcgccggtcc gcgatggcac tctccaggcc gctgatgcgc
    21601 gactgcaggt gcaggaactg ttcgttggcc ttcaggtccg ggtacgactc ggccagcgcg
    21661 aacaggttgc ccagtccgct gcgcaaggcg ccttcggcca ggcccagggc gccgatgtcg
    21721 tgcgactcgc gcgcatcggc cacccgtgct cgcgcctgga tcaccttctc cagcgtgagc
    21781 tgctcgtgct gcatgtactg cctgcaggta tccaccagct tgggcagttc atcgtggcgc
    21841 tgcttgagca gcacgtcgat gttggcccag gccttggaga cggcgttctt cacgctcacc
    21901 aggctgttga agaggatcac cccgtacacc agcaggatcg gtaaagaaat ccacaagatc
    21961 gttgccactc aggcctccct cgattggtca gaaccagtct cccgccggag gttaccgggc
    22021 aggcagcgcg cacgtgttcg gaatagacgg tggttgatcg cggtattggc gggattcgag
    22081 gtctggttct tcatggcaga ggagactaca tttgtggagg cggtggccgg ccgcccggcc
    22141 acacatccca tgcccacccg ccccacacgc ctgctcaagc ttcagctgct ggccgttttc
    22201 atcgcgttga acaccgtcgc ggcgctctgg gtcgcgagca cgctgtccga ggaccgtgac
    22261 taccgcgtgc agaaggccga gtccacgacg cagaatctcg cgctggtgct cgaccagaac
    22321 atcaccacgg cggtcgagaa gatcgatctg gccctgctca gtctcgtcga cgagttcgag
    22381 cgacagtttc gagaaggcgg gcgactggat gccgtgcgcg ccaatgaact ggtgctgacc
    22441 tacacgaagc gtctcgaggg cctgaacgga atccggatcg cagacgcgga aggaacggtg
    22501 gtgctgggga cggacgtggt gcctcaggcc cgtgtcagtt gggccgaccg tccgttcttt
    22561 ccgaagatgc gcgaccatcc ggatggcggg ctgctgatca ccccgccgct gctgggccgc
    22621 gtcagcgggg tctggatgat ctccttcaac cggcgcatca accgtcccga tggcactttc
    22681 ggcggaatcg tgtccgcgag cgtgccgctg gaacatctga gggcactggt ggccatgccc
    22741 aacctcggat cgcatggtgt ggtggcgctg cgcgatgccg acttcggcct gatcgtgcgc
    22801 catcccgcgc ttccgacgct gccgagtggt gtggtgggca gccggaatct gccgcccgaa
    22861 ctggcgactc tggttgcggc cgggcagacc cgtgcgacct accacacgca acacacggcc
    22921 gatggggtcg aacgcatcct ttcgtatcgt cgaatcgagc ggggccggtt ctcgctgctg
    22981 gtcggcatgg gcagcgagga ttacctggcc gaatggcacg cctcggtgcg cgactcggta
    23041 ttgcgactcg cgctgttcgc ggtctggtct ggcttgggtt gctggctgct ctggaattcg
    23101 acccggcagc tgcggcaggc caatgaacgc agccaggcgc tgctgcgcgg tgccagcgac
    23161 ggcatccatg tcctggatga gcagggcctg gtggtcgagg ccagcgatgc ctttgcgcgc
    23221 atgctcggtc ggcgcctcga agatgtgatc ggcatgcacc tgtcgcagtg ggatgcccac
    23281 ccggaaaccg gtggaatgtt gccgcagttg aagctcatgc aggaacatgg cgagcaggcg
    23341 ctgctcgaga cgcgcctgcg tcgcgccgat ggcagtctgc tggacgtcga gatcgccgcc
    23401 tacgccatat cgctcgatga ccggccgatg ctgttcgcct cggcgcgcga catcaccgag
    23461 cgcaagcagt ccgaactggc gatccgcgaa ctcaacgccg atctcgagcg gcgcgtgaga
    23521 cagcgcaccg cagagctcga gatcgtcaac gcggggctgc tgcaggcgtg cgacgcggcg
    23581 caggcggcca accgggccaa gagctccttc ctggccaaca tga
//
LOCUS       DAOORV010000051        23056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2706_length_23056_cov_7.207295, whole genome shotgun sequence.
ACCESSION   DAOORV010000051 DAOORV010000000
VERSION     DAOORV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_23056_cov_7.207295"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKV56_08705"
     CDS             <1..313
                     /locus_tag="PKV56_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOX67936.1"
                     /translation="FVTVGAAGGKRLPQAVEVTDLSNTTHDPLLASLRQRLRQHHGAA
                     RSGRIGVNCVYSREAVHQPEQSCEIEGNLNCHGYGSSVTVTATFGMVAASEAIHALLA
                     Q"
     gene            374..449
                     /locus_tag="PKV56_08710"
     tRNA            374..449
                     /locus_tag="PKV56_08710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Lys,seq:ttt)
     gene            487..562
                     /locus_tag="PKV56_08715"
     tRNA            487..562
                     /locus_tag="PKV56_08715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:520..522,aa:Lys,seq:ctt)
     gene            complement(704..1552)
                     /locus_tag="PKV56_08720"
     CDS             complement(704..1552)
                     /locus_tag="PKV56_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67937.1"
                     /translation="MNEAAPVHQHGIETRPVSIPAPLPIVCMAIGDLYGLADTYITRM
                     FEMLTRHCPVPFRLVCYTDLPRNAPGGVEFRDCSGWNELERDGMRATTRKLGLFNPDY
                     VEFEEYLYLDLTLVIRQSMEALIHHAFSRPQDLIVVEDWHHGGYNSSVMRIRPAPLRF
                     IYEAFVAGESFPFRVPGDQDFIRAAVLSRGEQARVAMFPDGWVVSFKGMMRIGRRDPV
                     RARDLIEHATVVKFHGQPRMHEAFDPLHHFFRIRLRDWAHGRLRSPISIADLKSHWVS
                     RLASER"
     gene            1749..2393
                     /locus_tag="PKV56_08725"
     CDS             1749..2393
                     /locus_tag="PKV56_08725"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX67938.1"
                     /translation="MQQTQIESAHYDFARYVSAKRWASYWHQVRETLALRPETVLEVG
                     VGTGIYRSVLRGLKVSVSSLDINPALQPDHVGSVTRMPFADNSFSVVVAFQVLEHLSY
                     DEFVPSVSEMRRVAASHVVLSLPDARKVWTYLFTLPWLGECRFQVPKPRLRPRLHRAT
                     GQHLWEISKRGYDVRRIVDDLQRCGLQMVSTYRAPENPYHRFFVCRKVDAPSGR"
     gene            complement(2405..3238)
                     /locus_tag="PKV56_08730"
     CDS             complement(2405..3238)
                     /locus_tag="PKV56_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOX67939.1"
                     /translation="MHSILSLLGIESWKPALTALVLPPVPLLLTLLVGARLLISRRAL
                     GWLIVLASVGGIWLSACSGFARMIEPALLTASPPLRPDRIAELRAEAKSAPDKRGGTS
                     RVAILVLGGGVEPYAPEYGTSNLASYSMESLRYGMWLSRETGWPVAFSGGIGWSNRPG
                     AAEADVAARIAAQEYGRPLRWVESASRDTRENAARSLPMLKEAGVTHVLLVTRGWHMQ
                     RAKRLFDEAALGSGMRIDAAPMGLSSGVEIPILAWMPSSAGFERVRLVLREWLGWKFG
                     A"
     gene            3370..7215
                     /locus_tag="PKV56_08735"
     CDS             3370..7215
                     /locus_tag="PKV56_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOX67940.1"
                     /translation="MKWLRRFAWAVIGLLGLWALAWLAVPPLLKSQAQQRLGAALGRT
                     VTLGEVDFSPWSLELTVRGIAIGGVPADQPLPAGGAPALLKVARLYVNADISSLLRRA
                     PVIEAFEVDGPELRLTRTADGHYDIDDLIARFKPAEPPPPTSAPVLFALYNLKLRDGA
                     VVFEDRPVDAVHRLTAINLSLPFLANLPAHIDVKVEPRLAFRLDGTAFDTGAQTTPFA
                     QSRAATLQFRMGDLNLKPFLGYLPQALPFRLHQGLASGELDLDFRMPAGTAPIVVLRG
                     KVGLRNVEASDPAGAPLLAWKRLDVALRDSQPLKRALSFGTIELDAPTLHVTRDASGN
                     LNLAQVSGPSPVAPPAAEGAGSQSSAPAVVPVSPWKVQVEALHISGWQVLWTDSGLTP
                     AAALALKDIDLKAGPVDYPFATDAAMPLSLMATLAAAGADAPAQGRFSVEGRVSDTRA
                     LLDLRLDGLTLDALAPYLAESLQPKVSGVVTASAQLTWAAGNQPKLQVGRGELVLDGL
                     RVTEHDAAASQRKAAAGPAAMSVKKLIVQGADVDLQAHTLSVASLKILQPAAAVSRDQ
                     AGLWNVQRWLKPAGATKAAVAAPAESAGPPPGAASAPAQGELPWRIELGDFSIDGGSL
                     RLDDAFVAGLPDTEPVRLQLAALKFGVQNLALSGARTTAPARMQFSARLLDPREGDQT
                     SVKAGLATAGSLDWRGQLGLQPLMVQGSAKLERFPLQAFEPYVRDRLPVSLMRAEAGL
                     KADISVKDLPSGWDVGVRGDLLLADVLVNSRPAAGTLTGHGSTDELLSWQSFRLNGVT
                     LALAPGQSPRVAIDEAVLADFYSRLVITEQGRFNLQDVGPAEAAAASAEAGAAATPPA
                     AVVSSTAPQASVPQAASRGADPAFELSVGATRLVNGRIDFTDRFVRPSYSADLTQLNG
                     TLGAFRSGSREMAALELRGRAAGTALLDISGQLNPTAEPLALNIRARATDLELAPLSP
                     YAGKYAGYAIERGKLSMDVSYAISADGRLEAKNQIVLNQLTFGERIESPSATKLPVLL
                     AVALLKDRHGVIDINLPISGSLDDPQFSVGGIIFKVIVNLITKALTAPFSLLFGGSSE
                     DLSQVDFQPGVTALTESGASALDKVAQALIDRPSLKMTVTGVSDPAAERAAFVQAALD
                     ARLMQELKKEAARAGAPAVAASATASPQAPAGAERERLLKAVYRQTDIPDKPRNLIGL
                     AKDIPAAEMESLLKSRIAVSDEGMRELALQRGLVVRDALIAKGLPSERLFIASPKMRA
                     QEASTEPWVPSARLSLTNN"
     gene            7244..9379
                     /locus_tag="PKV56_08740"
     CDS             7244..9379
                     /locus_tag="PKV56_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX67941.1"
                     /translation="MPIRTRTTLSFSVLAMACSAQAQSTPAAPASVGQLDTIVITGQR
                     TREPSFDVPAAISAVTRDTIENGGPQVNLSEALNRVPGISILNRNNYSQDLQMSIRGF
                     GARSTFGIRGVKVLVDGIPATMPDGQGQVSNISLSSVGRIEVLRGPFAQLYGNAAGGV
                     VQVFTEDDALVPTASVSATLGRFGMWKVGTKLSTSTPGYGLTIDASEFRTDGFREHSE
                     ARRRQFNARWQSQLTDSTHVSVVLNALDQPVSQDPLGLNAAQFRSHKAGTNPFYLAAL
                     AQDPRKDVHQEQLGTVIDHRLNENTDLSGRLYIGSRKLDSALSVPISAQGSPTSSGGI
                     VAFDRTYGGGAGQIAHRVPLGEGRQLKLTAGVEVEAFSEDRQGYVNTLGERGEIKRDE
                     KNKVHTEDFYAQAAYDITSQWTVTAGARHSKVKFKSKDHYIAAGNPDDSGKLDFSATN
                     PVLGVAWKVTPTLNAYANIGRGFETPTFNELSYRPGGQTGLNTDLSASRSRHAEIGAK
                     WKYASRQRLDVALFDIRTKDELVVDTNTGGRSTFKNAGHTSRRGIELSHVGYITDALR
                     STVSLSAMRARFDDPFISGAGATPNVFSGNHLPGTPERNLFAELVWSPANAWAGFSGG
                     AEVVHTAKLYVNDTNTDATSSSTVFNLRASFKQKFGGWEFSEMIRVDNVTDRYYAGSV
                     IVNEGNSRFFERALPRNWTAGITAKYALE"
     gene            9488..10024
                     /gene="msrA"
                     /locus_tag="PKV56_08745"
     CDS             9488..10024
                     /gene="msrA"
                     /locus_tag="PKV56_08745"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOX67942.1"
                     /translation="MALEVITLGGGCFWCTEAVFEHVDGVTAVESGYCNGQVARPTYE
                     QVCTGRTGCVEAIKVTFDNERISLREVLEIFFVIHDPTTLNRQGADSGTQYRSGIYFH
                     DPEQQRIAREVMDEVDRKVGGKLVTELVAVADYSRAEEYHQHYYAQHPDQGYCAMVAA
                     PKIEKFRKTFASRFRQVA"
     gene            10021..10452
                     /locus_tag="PKV56_08750"
     CDS             10021..10452
                     /locus_tag="PKV56_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67943.1"
                     /translation="MTSRRACILALCCCAILMQSIGLLHTYVHPRGTPSLHALDCESG
                     SAQPTPPTACLEQPSHGLTERAPGSLAGSEWVHGLFAGHDSASSDCSLFDQLTHADLL
                     HDLPTLSLAHAAPRHPDAVHAAWHHATQATGFLARAPPAIG"
     gene            10622..12706
                     /locus_tag="PKV56_08755"
     CDS             10622..12706
                     /locus_tag="PKV56_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX67944.1"
                     /translation="MIQRFHLRPRVLVRALGLVCTVGAPVAGLAQATTSVPAVRTLDT
                     VVITANPLGSARIAAPVSVLSGDGLVLRRGSSLGETLDGMPGVSSTYFGPNASRPVIR
                     GLDGDRVRILSNAGASLDASSLSYDHAVPIDPLIVDRVEVLRGPGALFYGGSAVGGVV
                     NALDNRIPRTPLTGLSGAAEVRLGGADRERGGAAIIETGNGQVALHADVFGRDSSNLR
                     VPRYTPIEGGERLDRSGRVRNSASETRGGALGGSVFFGDGSISGRAGLAVDTYDSDYG
                     VTAEPDVTIKMKRDHVGFASELKGLGGPIRVLRVNANYADYQHKEIEGSGEVGTVFSS
                     EGHDFRIEAEHAPIGTLRGVIGVQHEDFDFSALGEEAFVPGTRTRKTGLFALEELPWS
                     GGTLSAGFRVERAHVDSNGDSAGAEPKFGASVERSFTLRSLSLSNVYPLSSNWSLSGT
                     LSSTERAPTYFELYANGVHAATGAYEQGSTTLPKERGTNIDVALEWKTEVDRLRVGVF
                     NTRFSRFISLDASGTQVDESGVPAADGATDGVPLYRFSAVRARLHGVEIEGRKRLADL
                     PWTLDATTRFDATRATNRSTGEPLPRVSPWRLNLGLDAARGDWAARVEVDHTARQGRV
                     PDTDSPTDSYTIVNLSLSQRFSLDQADALWFVKLTNLGDELAYSASSIQTIRGLSPLP
                     GRAVKVGLRVTF"
     gene            complement(12722..13357)
                     /gene="pdxH"
                     /locus_tag="PKV56_08760"
     CDS             complement(12722..13357)
                     /gene="pdxH"
                     /locus_tag="PKV56_08760"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOX67945.1"
                     /translation="MTNIADLRKSYERAELDESASHADPLLQFEGWLQQALNAELPEP
                     NAMTVATVGPEGRPSTRVVLIKGCDSRGIVWYTNYESRKGRELAAHPQAALQFHWVEL
                     ERVVRIEGRVEKVDAAESDAYFASRPLDSRIGAWASPQSEVIPSRALLVANAAKYAAR
                     YLLHPPRPPHWGGFRLVPDFWEFWQGRPSRLHDRLRYRLDGGAWIRERLAP"
     gene            13512..14162
                     /locus_tag="PKV56_08765"
     CDS             13512..14162
                     /locus_tag="PKV56_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX67946.1"
                     /translation="MSSGPVIIVLAAGLGSRFQDAGPKLSQPLGVTSVLGATLAQAQA
                     SQLPVVVVTTEALVDEAARHVARQDIVVLEAASARPVQGRGDLRAMNARLGMGASIAA
                     GVSVSSNASGWLILPGDMPMLQFSTLQRVAAALRDHAVAYAQHLGRRGHPVGFSGELY
                     SELIQLQGDDGARRLVSRYPAAAIEVDDPGVLVDVDTVADLAQVRALWARAALAAG"
     gene            14276..16108
                     /gene="edd"
                     /locus_tag="PKV56_08770"
     CDS             14276..16108
                     /gene="edd"
                     /locus_tag="PKV56_08770"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HOX67947.1"
                     /translation="MPMNPAVVRVTQRISERSAPTRKAYLARVASAGQRPRGSERMGC
                     ANVAHAFAALPINDKLRVVTERAPHIGVVTAYNDMLSAHQPYEGFPAVIRDEAHRLGA
                     TVQVAGGVPAMCDGVTQGMPGMELSLFSRDTIAMGTAVALTHDVFDAALLLGVCDKIV
                     PGLLIGALHFGHLPCVFVPAGPMSTGLSNNAKGKVREQYAQGLVGRDELLQAESAAYH
                     GAGTCTFYGTANSNQMLLEAMGLHVPGAAFVHPHAELRETLTRDAVRSVLGLVKARRY
                     MPIGQLVDERVIVNAMVALLATGGSTNHLIHWVAVARVAGILIDWTDFSELSHATPLL
                     ARVYPNGSADVNQFQAAGGPGYVIRELIDAGLMHADVATVSDGGLRDYTRIPERTEAA
                     ALAWRDLPPARTAQHDDDIVRPAAAPFSDHGGLALLIGNLGRAVIKTSAVPADRHAIE
                     APAIVFDTQEALQTAFKAGELDRDFVAVVRFQGPRANGMPELHKLTPPLAVLQGKGFK
                     VALVTDGRMSGASGKVPAAIHVSPEVLAGGPLGKVRTGDLIRLDSNAGTLEALVDAAT
                     WAARSQAELSAEQAEVNAHGLGRELFGGMRRSVLTAEEGAITWL"
     gene            16161..16787
                     /gene="eda"
                     /locus_tag="PKV56_08775"
     CDS             16161..16787
                     /gene="eda"
                     /locus_tag="PKV56_08775"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOX67948.1"
                     /translation="MQALELAAFGPVIPVIVIHRFEDAVPLAQALVDGGVRVLEVTLR
                     TPVALKCMEAIVRAVPGAIVGAGTVRCVADVRAAKDAGCQFAVSPGYLSSIGQVCREI
                     DLPLLPGVATSSEVMQANADGYDFLKFFPATAAGGIPLLKAFAGPFADVAFCPTGGIT
                     LESAPQFLALPNVKVCGGSWLTPPDAVASGDWARITRLAVEASALRGA"
     gene            complement(16809..17195)
                     /gene="gloA"
                     /locus_tag="PKV56_08780"
     CDS             complement(16809..17195)
                     /gene="gloA"
                     /locus_tag="PKV56_08780"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HOX67949.1"
                     /translation="MRLLHTMLRVGNLQRSIDFYTRVMGMTLLRTTERPEQKYSLAFV
                     GYGSNPEHAEIELTYNHGVDHYELGTAYGHIAIAVPDAHEACARIRTGGGAVTREAGP
                     VAGGSTVIAFVTDPDGYKIELIERRD"
     gene            17306..18547
                     /locus_tag="PKV56_08785"
     CDS             17306..18547
                     /locus_tag="PKV56_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX67950.1"
                     /translation="MTRLAPRTLSLGLVLLAAGGCFALTMGVRQSMGLFLGPINTHTG
                     LGLASISLAFAFGQLWWGLTQPFAGMVADRIGAGRVIVCGVLMVAVGTALIPHMSSAA
                     GLVFAIGVLAAGGAGIAGPSVLMAAATRLISPAQRGMATGIVNAGGSFGQFVFAPLAQ
                     GVTVVAGWMSSLHVLGALTLLALPAAWLLRGNALHLAAPAPGQQVAETTRQALSRALG
                     DRSFRLLCAGFFVCGFHVAFLSTHLPGVVASCQLSPTVGAWALGTVGLFNIIGSIGIG
                     WLMSYRAGYWRLKTLLTWIYAARAVAVLVFLLSPKTEAVMLVFAAVMGLTYLSTVPPT
                     VGLVAKFFGPSHMATLYGVAILVHQIGAFFGAYLGGKAFEWTGSYDWMWYADIVLAVG
                     AALVNLPIREARSVPLGAQPG"
     gene            18661..19434
                     /locus_tag="PKV56_08790"
     CDS             18661..19434
                     /locus_tag="PKV56_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOX67951.1"
                     /translation="MKIQSKFKADARCAVAVALAAGFSVSAWAGDDDSERSSCAGLPT
                     HAQLKTELTKARNASNGGFNLDMWGTVVNRDGVVCAVAYTGANRGSQWPGSRAISAQK
                     ANTANAFSLPGLALATANLYSAVQPGGSLFGLQESNPVDTDNAYKGPAQNWGAPNDPM
                     VGKRVGGINVFGGGLALYNASKQLVGAIGVSGDSSCADHAIAWRARSTLSLDYVPGGV
                     GPGGVDQLSYSGPWSQPHCVPGGAVAEDAVVLGLPATRK"
     gene            complement(19567..20121)
                     /locus_tag="PKV56_08795"
     CDS             complement(19567..20121)
                     /locus_tag="PKV56_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOX67952.1"
                     /translation="MNSAHQNVPSQTTSSGSVPRAVWIGGGLLCLVTAGLAGALITRT
                     VDSSPPAQGSPMLAEANAAQPASSTCPNCGIVESVTAVKQKGQGTGIGAVTGGVLGGV
                     VGNQFGSGSGKTAMTVLGAVGGGVAGHEVEKQVRSETHFNVKVRMDDGTTRTFRRSQS
                     MAVGTQVTVDGDTLSVTKKHSQAQ"
     gene            complement(20287..21276)
                     /locus_tag="PKV56_08800"
     CDS             complement(20287..21276)
                     /locus_tag="PKV56_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX67953.1"
                     /translation="MEPIYPFSTPLGQGIWAVDTGYQRPNFDAAYLIVEQGRAAFIDC
                     GTSHSVPRLLAALDATGVPRDAVDWVIPTHVHLDHAGGAGALMAALPSARLVVHPRGA
                     RHLISPDALLAGARAVYGEAEVQRAYGTVVPVAADRVTESSDGLVIRLAGRPLRFLDT
                     PGHARHHHCIWDERSRGFFTGDTFGLSYREFDTSAGIWLLPTTTPVQFDPAALRTSVE
                     RLLAEVPACMYLTHYGRVPVIGSENPSDVQQLGRELLEQLDEVVALCGQAAGLGLPAR
                     ERHAFLRDGLSALYLQRVAQLECQLQRKQILDMLEMDIELNAQGLGVWMDAKR"
     gene            21356..21916
                     /locus_tag="PKV56_08805"
     CDS             21356..21916
                     /locus_tag="PKV56_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67954.1"
                     /translation="MPAYAPVLPTGVLLSLRAPHPLAKTRCRLPATLAPIILALLAGC
                     SAYSPSALRPGATLAEVVSVMGPPTGSVQAPRRLEFARGPYGKHTYMADFDADGRLLS
                     WEQVLTENNFFQVQIGETKEAVVERLGRPSSTFPIGRQRIVVWNYRYETPFCQWFQVS
                     IGTAPASLGRVTDVGFGPDPMCTSRE"
     gene            21936..22286
                     /locus_tag="PKV56_08810"
     CDS             21936..22286
                     /locus_tag="PKV56_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HOX67955.1"
                     /translation="MTIILYGIPNCDTVKKARVWLQAQGLEFTFHDFKKQGVPLPRLD
                     AWLESVGWESLLNRKGSTWRKLDPAKQDAVQDAASARALMVAQASIIKRPVVEYSGGL
                     LVGFDPQEWGRALA"
     gene            complement(22359..>23056)
                     /gene="acnA"
                     /locus_tag="PKV56_08815"
     CDS             complement(22359..>23056)
                     /gene="acnA"
                     /locus_tag="PKV56_08815"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOX67956.1"
                     /translation="FGDSITTDHISPAGSIKEDSPAGAWLAGHHVLKADFNSYGSRRG
                     HHDVMVRGTFANVRIKNLMIPAGADGSREEGGVTLLQPSGEKAFIYDAAMTYMAAGTP
                     TLIFAGEEYGTGSSRDWAAKGTALLGIKAVVARSFERIHRSNLVGMGVLPLQFKGTDS
                     WQSLGIRGNELFDIELAAHIQPQQDATLVITSADGRKRRVPLTLRIDTPIEVDYYQHG
                     GILPFVLRQLLTA"
BASE COUNT         3698 a         7568 c         7761 g         4029 t
ORIGIN      
        1 ttttgtcacg gtgggcgctg cgggcggcaa acgactgcct caggcggtcg aagttaccga
       61 tctctcgaac accacgcatg atccgttgct ggcctcgctg cgtcagcgcc tgcgccagca
      121 ccatggcgcc gcccgaagcg gtcgcatcgg cgtgaactgc gtctactcgc gtgaggccgt
      181 gcaccagcca gagcagtcgt gcgagatcga gggcaacctc aactgccatg gctacggctc
      241 gagcgtgacc gtcacggcga ccttcggcat ggtggccgcc agcgaggcga ttcacgcgtt
      301 gcttgctcag tgagcggcgg caatcggccg tgccgagccc cgaaaaatcg ggctatactt
      361 atgggctgct cgggggttgt tagctcagtt ggtagagcag cggactttta atccgtaggt
      421 cgtgggttcg agccccgcac aacccaccac cgtcacgtac aggttttacc ggcacatcga
      481 aatgtcgggc tcttagctca gttggtagag cagcggactc ttaatccgta ggtcgagtgt
      541 tcgagtcact cagggcccac cagtaaaaac aacggcttgc cagaaatggc aggccgtttg
      601 tctttgtggc gcagcttttt cgttgtgtcc cggggcagtc agtcctgtcg gctccggatc
      661 ggacagtccg aacgctctgc cgctgcgctg acggcgtgcc gcgtcagcgt tccgatgcga
      721 ggcggcttac ccagtgagac ttcagatcgg cgatggagat cggtgagcgc aagcggccgt
      781 gggcccaatc gcgcagccgg atgcggaaga aatgatgcag cgggtcgaag gcctcgtgca
      841 tgcggggctg gccgtgaaac ttcacgacgg tcgcgtgctc gatcagatcg cgtgctcgta
      901 cggggtcgcg tctgccgatc cgcatcatgc ccttgaagct gacgacccag ccgtcgggaa
      961 acatcgcgac gcgggcctgt tcaccgcggc tgagaaccgc cgccctgatg aaatcctgat
     1021 cgccgggcac ccggaacgga aacgattcgc cggcaacgaa tgcttcgtag atgaatctca
     1081 gcggtgcagg ccgaatgcgc atcacgctgg agttgtagcc gccgtggtgc cagtcctcga
     1141 ccacgatcag gtcctgcggc cgggagaagg cgtggtggat cagggcctcc atcgactggc
     1201 ggatcaccag cgtcaggtcg aggtagagat attcctcgaa ttccacgtaa tcgggattga
     1261 acaggccgag cttgcgcgtt gtggcccgca tgccatctcg ctccagttcg ttccagcccg
     1321 agcagtcacg aaactcgacg ccgccaggcg cattcctggg cagatcggtg tagcagacga
     1381 gacgaaacgg gaccgggcaa tgccgggtca gcatctcgaa catgcgagtg atgtaggtgt
     1441 cggccaggcc gtacagatcc ccgatggcca tgcacacgat gggcagcggc gccgggatcg
     1501 ataccgggcg cgtttcgatg ccgtgctggt gcacgggtgc tgcttcgttc actgtctgag
     1561 atcctcgttg cggcccattg cgcggtcggt gtaccacgcc cggctgccgg agcgatcgcg
     1621 ccgatcacgc gtgccatact tggctggaat cgcgattcag ctcggtcgat ccttgcgccg
     1681 atagctgcat tttcgccccg gtggcaagcc ccggtccccc gcagccgaaa cactattctg
     1741 ctacacgcat gcaacagact caaatcgagt cggcgcacta tgactttgct cggtatgtca
     1801 gcgccaagcg atgggcgtcg tactggcacc aggtccgtga aacactggcg ctgcggcctg
     1861 agaccgtgct cgaagtcggc gtgggcacag gcatctaccg gtcggtgctg cgcggtctga
     1921 aggtgtctgt cagcagtctc gacatcaacc cggcgctgca acctgatcac gtcggcagcg
     1981 tgacccgcat gcctttcgcg gacaacagct tttcggtggt cgtcgcgttt caggtcctgg
     2041 agcacctgag ttacgacgaa ttcgtgccca gcgtgtccga gatgcgccgg gtggcggcca
     2101 gccatgtcgt cctttcgctg cccgacgctc gcaaggtctg gacgtacctg ttcaccttgc
     2161 cctggctcgg cgagtgccgc ttccaggttc ccaagccacg cctgcgtccg cggctgcatc
     2221 gggccacggg ccagcatctc tgggagatca gcaagcgcgg gtatgacgtg cggcgcatcg
     2281 tcgatgacct gcagcgctgc ggattgcaga tggtcagcac ctaccgggcg ccagaaaacc
     2341 cctaccaccg cttcttcgtc tgtcgcaagg tcgacgcccc gtcgggccgg tgagcggggg
     2401 ctgatcaggc cccgaacttc cagccgagcc attcgcgcag gaccagacgc acgcgctcga
     2461 aaccggcgga tgagggcatc caggcaagga tcggaatctc gacacccgat gacaggccca
     2521 tgggtgcggc atcgatgcgc atgccgctgc caagcgcggc ttcatcgaac aggcgctttg
     2581 cgcgttgcat gtgccagccg cgcgtgacca gcagcacatg agtcactccg gcttccttca
     2641 gcatcggcag gctgcgggcg gcgttctcgc gggtatcgcg ggacgctgat tccacccacc
     2701 gcagtggccg accgtattcc tgggcagcga tccgggccgc gacatcggcc tcggctgcgc
     2761 caggccggtt gctccagccg atgccgccgc tgaaggcgac cggccagcca gtttcgcggc
     2821 tcagccacat gccgtagcgc aggctctcca tcgaatagga cgcgagattg ctcgtgccgt
     2881 attcgggtgc atagggttcg acacctccac ccagcaccag gatcgcgacc cgactcgtgc
     2941 cgccgcgctt gtccggcgct gatttcgcct cggcccgcaa ctcggctatc cggtcaggtc
     3001 gcagaggcgg cgaggcagtc agcagtgccg gctcgatcat ccgggcgaaa cccgagcagg
     3061 cgctgagcca gatcccgcct acgctcgcca gcacgatcaa ccagcccagg gcgcgcctcg
     3121 atatcagcag tcgagcgccc acgagcaggg tcagcagcaa cggcaccggt ggcagcacga
     3181 gggcagtcag cgcgggtttc cagctctcga tgccgagcag gctcagtatg ctgtgcaagg
     3241 tgcggcgctt tcggttcggg tcggcaaagt ggcattgtga gcgggcatct gcccgtgatg
     3301 cgacggcgcg gcagcgccgg atgggttgtc tcgagccgta gccggcttca agccatgagg
     3361 agtcgaagat tgaagtggtt gcgacgtttt gcgtgggcgg tgatcggatt gctgggcttg
     3421 tgggcgctgg cgtggctggc cgtgccaccg ctgttgaagt cgcaagcaca gcagcggctt
     3481 ggtgcagccc tgggccggac cgtcacgctc ggcgaagtcg atttcagccc ttggtcgctc
     3541 gaactgaccg tgcgcggcat cgcgatcggt ggcgtgccag ccgaccagcc gctcccggcc
     3601 ggaggtgccc ctgcgctact gaaggtcgcg cgcctctacg tcaacgccga catcagctca
     3661 ctgttgcgcc gtgctccggt gatcgaggca ttcgaggtcg atgggcctga attgagattg
     3721 acgcgcacgg cagacggtca ctacgacatc gatgacctga tcgcccgttt caagccggct
     3781 gaaccgccgc ccccgacatc cgcgccggtg ctgttcgcgc tctacaacct gaagttgcgc
     3841 gacggggcgg tggttttcga ggaccggcct gtcgatgcgg ttcaccgcct gacggcgatc
     3901 aacctgagcc tgccattcct ggccaacctg cctgcccaca tcgacgtgaa ggtcgagcct
     3961 cgcctggcct tccggctgga tggaactgcc ttcgacacgg gagcgcagac gaccccgttc
     4021 gcccagagcc gcgcggccac gctgcagttc aggatgggcg acctcaatct gaagcccttt
     4081 ctgggttacc tgccgcaagc gctgccgttc aggctgcacc agggcctggc atcgggcgag
     4141 ctcgatcttg atttcaggat gcctgccggt accgctccca tcgtcgtgct tcgcggcaag
     4201 gtcggactgc gcaacgtcga agccagcgat ccggcaggcg cgcccttgct tgcatggaag
     4261 cgactggatg tggcccttcg cgacagccag ccgctcaaac gcgcgttgtc gttcggcacg
     4321 atcgaactgg acgcaccgac attgcatgtc acccgtgacg cctcggggaa tttgaacctg
     4381 gcgcaggtgt cgggccccag cccggtcgcg ccgcctgctg cagagggcgc tggatcgcag
     4441 tcgtcagccc cggctgtcgt gccggtttcc ccctggaagg tgcaggtcga ggccttgcac
     4501 atcagcggct ggcaggtgct ctggaccgac tccggcctga cgcccgccgc ggcgctggcg
     4561 ctgaaggaca tcgatctgaa ggctggccct gtcgactacc ccttcgccac agacgccgcg
     4621 atgcccttga gcctgatggc cacgctggca gccgcgggcg cagatgcgcc tgctcaaggg
     4681 cgcttctcgg tcgaaggccg tgtcagcgac acgcgtgcgt tgctcgacct gcggctggac
     4741 gggctgacgc tcgatgcgct cgcaccgtac ctcgccgaat cccttcaacc gaaggtcagt
     4801 ggcgtggtca ctgcatccgc tcaactgacc tgggccgcag gcaaccagcc gaagctgcag
     4861 gtcgggcggg gagagctcgt tctcgacggt ctgcgggtga ccgagcacga tgccgctgct
     4921 tcgcagcgca aggccgcggc cgggcctgcg gcgatgtcgg tgaagaagct gatcgtgcag
     4981 ggtgccgatg tcgatctgca ggcgcacacg ctgagcgtgg cgagcctgaa gatcctgcag
     5041 cctgcagccg ccgtgtcgcg cgaccaggcc ggcctctgga atgtgcagcg ctggctcaag
     5101 cccgcgggcg caacgaaggc ggcggtcgcc gcgccagcgg aatccgccgg gccgcccccg
     5161 ggtgcggcat cggcacctgc gcagggcgag ttgccgtggc ggatcgagct gggagacttc
     5221 tcaatcgatg gcgggtccct gcgcctggac gatgccttcg tcgccggcct tccggacacc
     5281 gagccggtcc gactccagct ggcggcgctg aagttcgggg tgcagaacct ggcactttcc
     5341 ggtgcccgca ccacggcccc tgccaggatg cagttcagcg cccggctgct tgacccgcgc
     5401 gagggtgatc agacgtcggt caaggccggc ctcgccaccg ccggttcgct ggactggcga
     5461 gggcagctcg gcctgcagcc gttgatggtc cagggcagcg cgaagctcga gcgctttccg
     5521 ctgcaggcct tcgagcctta cgttcgcgac cgcctgcccg tgagcctgat gcgggccgag
     5581 gccggcttga aggccgacat ttcggtgaag gacctgcctt ccgggtggga cgtcggcgtc
     5641 cgcggcgatc tgttgctggc cgacgtcctg gtgaacagcc ggccggcggc gggcaccctg
     5701 accggtcacg gcagcacgga cgagctgctc agctggcagt cattcaggct gaacggcgtg
     5761 acgctggcac tcgcgccggg gcagtcgccg agggtcgcca tcgacgaggc agtgctggcg
     5821 gatttctatt cgcggctcgt catcaccgag caggggcgct tcaacctgca ggacgtggga
     5881 cctgcggagg cggcggccgc ctcggcggag gccggcgcgg cggcgacgcc gcctgccgcg
     5941 gtcgtcagtt ccacggcccc acaggcctcg gtgcctcagg cggcttcccg gggtgcggat
     6001 ccagcgttcg aactgagcgt cggcgccacc cggctcgtca acggacgcat cgatttcacc
     6061 gaccgcttcg tgcgtcccag ctacagcgcc gacctgaccc agctcaacgg aacgctgggc
     6121 gcgttccgat cggggtcgcg cgagatggcg gccctcgaac tgcgcggccg tgccgcgggc
     6181 accgcgctgc tcgacatctc gggacagttg aacccgaccg ccgagccgct ggcgctgaac
     6241 atccgcgcca gggcgacgga cctcgaactg gcacccctgt cgccgtatgc cggcaagtac
     6301 gccggctatg cgatcgaacg cggcaagctc agcatggacg tctcgtacgc aatctcggcc
     6361 gacggcaggc tcgaggcgaa gaaccagatc gtcctgaacc agctgacctt cggcgaacgc
     6421 atcgagagcc cgtcggccac caagctgccc gtgctgctgg cggtggcgct gctgaaagat
     6481 cgccacggcg tgatcgacat caatcttccg atcagcggtt cgctcgatga tccccagttc
     6541 agcgtgggag gcatcatctt caaggtcatc gtcaacctga tcaccaaggc gctcactgcg
     6601 ccgttttccc tgttgttcgg cggcagcagt gaagacctga gtcaggtgga tttccagccc
     6661 ggcgtcactg cattgaccga gtccggtgcc tctgcgctcg acaaggttgc gcaggcgctg
     6721 atcgaccggc cgtctctgaa gatgacggtg accggcgtgt ccgacccggc tgccgagcgc
     6781 gcagccttcg tgcaggccgc gctcgatgcc cggctgatgc aggagttgaa gaaggaggcc
     6841 gcacgtgccg gcgccccggc cgtggcggcc agtgccacgg cgtcgcccca ggcccctgcc
     6901 ggagccgagc gggagcggct gctgaaagcc gtctaccgtc agaccgatat tcctgacaag
     6961 ccgcgcaacc tgatcggtct ggcgaaggac attccagctg ccgagatgga gtcgctgctc
     7021 aagtcgcgca tcgccgtgtc cgacgagggg atgcgtgaac ttgcgctgca gcgcggcctc
     7081 gtggtgcgag acgcgctgat tgccaaggga ttgcccagcg aaaggctttt catcgcttcg
     7141 ccgaaaatgc gtgcgcaaga ggccagcacc gaaccctggg taccgagtgc ccggctgtcg
     7201 ctgaccaaca attgatgacc tgaaaaaaac agatcgagac aacatgccca tccgaacccg
     7261 aaccaccttg tcattcagcg tgctggcgat ggcctgctcc gcccaggcgc aatcgacccc
     7321 agcggcgccc gcgtcggtcg gacagctcga caccatcgtc atcacgggcc agcgcacgcg
     7381 cgagccgtcg ttcgacgtgc cagcggccat ctctgccgtg acccgcgaca ccattgaaaa
     7441 cgggggcccc caggtcaacc tgtcggaggc gttgaaccgc gtgcccggca tctcgatact
     7501 gaaccgcaac aactactcgc aggacctgca gatgtcgatc cgcggcttcg gtgcgcgctc
     7561 gaccttcggg atccgcggag tcaaggtgct ggtcgacggc atcccggcga cgatgccgga
     7621 cgggcaggga caggtctcga acatctcgct cagctcggtg ggtcgcatcg aggtgctgcg
     7681 cggaccattc gcccagttgt acggaaacgc cgccggcggt gtggtgcagg tcttcaccga
     7741 agacgacgcg ctggtgccga ccgccagcgt cagcgccacc ctcggacgct tcggcatgtg
     7801 gaaggtcggt accaagctca gtaccagcac gccaggttac ggcctgacga tcgatgcctc
     7861 cgaattccgc accgacggct ttcgcgaaca cagcgaggcg cgacgccggc agttcaatgc
     7921 acgctggcag agccagctga ccgattccac gcacgtgagc gtggtgctca acgcgctgga
     7981 ccagccggta tcgcaggatc cgctgggact gaacgccgcg cagttccgca gccacaaggc
     8041 cggcaccaat ccgttctacc tcgctgctct ggcgcaggat ccgcgcaagg acgtgcacca
     8101 ggagcagttg ggcaccgtca tcgatcatcg gctgaacgag aacaccgatc tgtccgggcg
     8161 tctctacatc gggtcgcgca agctcgacag cgcgctctcg gtgccaatct cggcgcaggg
     8221 ctcgccgacc tccagtggcg gcatcgtcgc cttcgatcgc acctacggcg gcggtgcagg
     8281 ccagatcgcg catcgcgtcc cgctgggcga gggccggcag ctgaagttga cggctggtgt
     8341 cgaggtcgag gccttcagcg aggaccggca gggctatgtc aatacgctcg gtgagcgtgg
     8401 agaaatcaag cgagacgaga agaacaaggt ccacaccgaa gacttctacg cacaggccgc
     8461 ctacgacatc acatcgcagt ggacggtgac cgccggtgca cgccacagca aggtcaagtt
     8521 caagtcgaag gaccactaca tcgccgcagg caacccggac gacagcggca agctggactt
     8581 cagcgcgacc aacccggtgc tcggcgtggc gtggaaggtc acgcccacgc tgaacgccta
     8641 cgccaacatc ggccggggct tcgagacgcc gacgttcaac gagctgtcgt accgtccggg
     8701 tggccagacc ggactcaaca cggacctgtc ggcatcccgc agccggcatg ccgagatcgg
     8761 tgccaagtgg aagtacgcga gtcgccagcg cctcgatgtc gcgttgttcg acatacggac
     8821 caaggacgag ctcgtcgtcg acaccaatac cggcggacgt tccaccttca agaacgctgg
     8881 gcacacctcg cggcgaggca tcgagctgtc gcacgtcggc tacatcaccg acgccctgcg
     8941 cagcaccgtc agcctgagcg ccatgcgcgc acggttcgac gatcctttca tcagcggagc
     9001 cggcgcgacg ccgaacgtgt tctcgggcaa ccacctgccc ggcacgcccg aacgcaacct
     9061 gtttgccgaa ctggtgtggt cgccggcgaa cgcctgggcc ggcttcagcg ggggggccga
     9121 ggtggtccac accgccaagc tgtatgtcaa cgacaccaac accgacgcga cttcgtcgag
     9181 caccgtgttc aacctgcgtg cgtcgttcaa gcagaaattc ggcggttggg agttcagcga
     9241 aatgatccgg gtcgacaacg tcaccgatcg ctactacgcg ggctcggtga tcgtcaacga
     9301 aggcaacagc cggttcttcg aacgcgcgtt gccgcgcaac tggacggcgg gcatcaccgc
     9361 gaagtacgca ctggagtgac gcggtaagga cttgcagccg caccccggca cagggtcatc
     9421 ctgggaagcg gcgccaccat tcggcgccgc ttccgataat cgggcgattc aatcgaaggg
     9481 gttctccatg gcgctggaag tcatcactct gggcggcggc tgtttctggt gcaccgaagc
     9541 ggtctttgaa catgtcgatg gtgtgactgc agtcgagtcg ggctattgca acgggcaagt
     9601 ggcccgaccc acgtacgagc aggtctgcac cggcaggacg ggctgtgtcg aggcgatcaa
     9661 ggtcaccttc gacaacgaac ggatcagcct gcgcgaggtg ctcgagatct tcttcgtgat
     9721 ccacgacccg accacgctga accgccaggg ggctgacagc ggcacgcagt accgctcagg
     9781 catctacttc cacgatcccg agcagcagcg aatcgcacgc gaggtgatgg acgaggtcga
     9841 ccggaaggtg ggcggaaagc tcgtcaccga actggtcgcg gtcgccgact actcgcgcgc
     9901 cgaggagtat caccagcact actacgcgca gcatccggat cagggctact gcgcgatggt
     9961 cgccgcaccg aagatcgaga agttccgcaa gaccttcgcg agccggttca ggcaggtggc
    10021 atgacgtcac gcagggcatg catcctggcg ctgtgctgct gcgccatcct gatgcagtcg
    10081 atcgggctgc tgcacaccta tgtgcaccca cggggcaccc cgtcccttca tgcgctggac
    10141 tgcgagtccg gttcggcgca gccgaccccg ccgaccgcat gccttgagca accttcgcac
    10201 gggctgaccg agcgagcgcc cggctcgctg gccgggtccg aatgggtgca cgggcttttt
    10261 gccggacacg acagcgccag ttccgattgc tcgctgttcg atcagttgac ccacgccgat
    10321 ctcctgcacg atctgccgac cttgtcgttg gcccatgcag cgccgaggca tcccgatgcc
    10381 gtgcacgcgg catggcacca cgccactcag gcgacgggct tcctcgcccg cgctccgcca
    10441 gcgatcggct gaacggcgct gcagcggtcc tcctgcggcc gccccggcgc cttccttcgg
    10501 ttcacacccg gcacgccccg gtccgccatg gacccgggcg ccatttcgct ttgtcgatcg
    10561 cctgatcgcg ggacgaccac gtcgctcgcg cgcgattcgc ctgttcagga tttcacgcat
    10621 catgattcaa cgatttcatc ttcggccccg cgtgctcgtg cgcgcactgg gtctggtctg
    10681 taccgtcggg gcgccagtcg ccgggctggc acaggccaca acgtcggtgc ctgccgtgcg
    10741 cacgctggac accgtcgtga tcaccgccaa cccgctgggc agtgcccgga tcgccgcccc
    10801 ggtctcggtg ctgtcgggcg acggcctggt gctgcgtcgc ggcagttcgc tcggagaaac
    10861 gctcgacggt atgcctggtg tttcgtccac ctacttcggc ccgaatgcca gccggcccgt
    10921 cattcgcggc ctcgacggcg atcgggtgcg catcctgtcg aacgcgggcg cttcgctcga
    10981 tgcgtcgtcg ctgagctacg accacgcggt gcccatcgat ccgctgatcg tcgaccgggt
    11041 cgaggtgctg cgcgggccgg gggcactgtt ctacggaggc agcgcggtcg gtggagtcgt
    11101 caacgcgctg gataaccgca ttccccgcac gccgctcacc ggcctgtcgg gtgcagccga
    11161 ggtgcgcctc ggtggcgccg accgggagcg gggcggggcc gccatcatcg agaccggcaa
    11221 tggccaggtg gcgctgcatg ccgatgtgtt cgggcgcgac agctcgaatc ttcgggtgcc
    11281 gcgctacacg cccatcgaag gtggggagcg gctcgatcgc agcggacgcg ttcgcaattc
    11341 ggccagtgaa actcgcggtg gcgcgctcgg tggttcggtt ttcttcggcg acggatccat
    11401 ctcggggcgc gccggtctgg ccgtcgacac gtacgacagc gattacggcg tgacggcaga
    11461 gcctgatgtc acgatcaaga tgaagcgtga tcacgtcggc ttcgccagtg agctcaaggg
    11521 cctgggaggt ccgattcgag tgcttcgtgt caatgccaac tacgcggact atcagcacaa
    11581 ggaaatcgag ggctccggcg aagtcggcac ggtgttcagc agcgagggcc acgacttccg
    11641 gatcgaggcc gagcacgcgc cgatcggtac gctgcgaggc gtcatcgggg tgcagcacga
    11701 ggatttcgat ttctcggcgc ttggcgagga agccttcgtg cccgggaccc ggacccgcaa
    11761 gaccggcctc ttcgcactgg aagagctgcc atggtccggc ggcacgttgt cggcagggtt
    11821 tcgggtggag cgtgcccacg tcgattcgaa cggcgattcg gctggtgccg agccgaagtt
    11881 cggcgcgtcg gtcgagcgca gcttcacgct gcgcagcctg tcgctctcca atgtctatcc
    11941 gctgtcatcg aactggtcgc tgtccggaac gttgagttcg accgagcgtg cgccgaccta
    12001 cttcgagctc tacgccaacg gggtgcatgc cgcgaccggc gcctacgaac agggcagtac
    12061 cacattgccg aaggagcgcg ggaccaacat cgatgtggcg ctggagtgga agaccgaagt
    12121 cgaccggctg cgtgtgggcg tgttcaacac gcggttctcg cgcttcatct cgctcgatgc
    12181 gagtggcacg caggtcgacg aatcaggggt gccggctgcc gacggcgcga cggacggtgt
    12241 gccgctttac cgcttcagtg cggtgcgagc ccgcttgcac ggggtcgaga tcgaagggcg
    12301 caagcggctc gccgatctgc cgtggacgct ggatgcgacc acccggttcg acgcgacccg
    12361 tgccaccaat cgcagcacgg gcgagccgtt gccgcgcgtc tcgccatggc ggttgaatct
    12421 cgggctcgat gcagctcgcg gggactgggc tgcgcgcgtc gaggttgatc acacggcgcg
    12481 ccagggacgg gtgcccgaca ccgattcgcc gacggacagc tacacgatcg tcaatctgtc
    12541 gctgtcgcag cgcttcagcc tggatcaggc cgatgcgctc tggttcgtca agttgaccaa
    12601 cctgggcgac gagctggcct acagcgcatc gtcgatccag acgatccgtg gcctgtctcc
    12661 actgccgggt cgggcagtga aggtcgggtt gcgcgtgacg ttctgacccg ttcgtgcggg
    12721 atcagggggc cagccgctcc ctgatccacg cgccgccgtc gagccggtag cgcagtcggt
    12781 cgtgcagccg cgatgggcgg ccctgccaga actcccagaa atcgggtacc agccggaatc
    12841 cgccccagtg cggcgggcga ggcgggtgca gcaggtaacg tgcggcgtac ttcgcggcgt
    12901 tggccaccag caaggcacgc gacgggatca cctcgctttg cggcgaggcc caggccccga
    12961 tgcgggagtc gagcggtcgc gaggcgaaat acgcatccga ttcggcggca tcgaccttct
    13021 cgacgcggcc ttcgatgcgc accacgcgtt ccagttcgac ccagtggaac tgcagggctg
    13081 cctgtggatg cgctgccagt tcccgtccct tgcggctctc gtagttggtg taccagacga
    13141 tgccacgcga atcgcagccc ttgatcagca ccacgcgcgt cgagggccgc ccctcgggtc
    13201 cgaccgtggc gaccgtcatc gcgttgggct ccggcagctc ggcgttcaat gcctgctgca
    13261 gccagccctc gaactgcagc agcggatcgg catgcgacgc gctctcgtcg agttcggcgc
    13321 gctcgtagct cttgcggagg tcggcgatgt tggtcatcga tcaagtatag aaagcactcg
    13381 ccggagcatt catgtcactg atggatacag ttttcttctg cggggaagga cttaagtgat
    13441 ggccctctgg cgcgaaaccg gccgcgcttc catcctgcac ccaacttgag aagaacaaga
    13501 gggaagtgcg catgagttcc gggcctgtga tcatcgttct ggcagcgggc ctgggctcga
    13561 ggttccagga tgccgggccc aagctcagtc agccgctggg cgtcaccagt gtgctgggcg
    13621 cgacgctggc gcaggcacag gccagccagc tgccggtggt cgtggtgacc accgaagcgc
    13681 tggtcgatga agccgcacgg catgtggcaa ggcaggacat cgtggtgctc gaagccgctt
    13741 cggcgcgtcc cgtccagggt cggggcgatc tgcgcgcgat gaacgcgcgg ctcggcatgg
    13801 gggcctcgat tgctgcgggg gtgtcggtga gctcgaatgc gagcgggtgg ctgatcctgc
    13861 cgggcgacat gccgatgctg cagttctcca ccctgcagcg ggtcgccgct gcgcttcggg
    13921 atcatgcggt cgcctatgcg cagcacctcg ggcgccgcgg tcatccggtg ggcttcagcg
    13981 gggagctcta ctccgaattg atccagctcc agggcgatga tggcgcccgg cgcctggtgt
    14041 cgcgctaccc ggcggcggcc atcgaggtcg atgatccggg cgtgctggtc gacgtggaca
    14101 cggtggccga tctggcgcag gtgcgcgcct tgtgggcgcg ggcggcgctc gcggcaggct
    14161 gagggcactt tcccgcgcga aaggcgccgg ccggcgccat gctggcgcgg ttgatgcaag
    14221 cggctgcgac actgtgagtc gcggcgaccc tgccgcagcc tggagagagc cgagcatgcc
    14281 gatgaatccc gccgtcgtcc gggtcaccca gcgcatcagt gagcgcagcg cacccacccg
    14341 gaaggcctat ctcgcccgtg tcgccagcgc cggccagcgc ccgcgcggct ccgagcgcat
    14401 gggttgcgcc aatgtggcgc acgcattcgc cgcgttgccg atcaacgaca agctgcgcgt
    14461 ggtcacggag cgcgcaccgc acattggcgt ggtcacggcc tacaacgaca tgctgtcggc
    14521 ccaccagccc tacgaaggct tcccggccgt gatccgcgac gaggcccatc ggcttggcgc
    14581 cacggtgcag gtggccggcg gcgtgcccgc gatgtgcgat ggcgtgacgc aggggatgcc
    14641 cggcatggag ctgagcctgt tctcgcgcga caccatcgcg atgggaaccg cggtcgcgct
    14701 gacccacgat gtgttcgatg ccgcgctgtt gctgggcgtc tgcgacaaga tcgtgccggg
    14761 gctgctgatc ggcgcgctgc acttcggcca cctgccgtgc gtgttcgttc cggccggtcc
    14821 gatgagcacc gggctcagca acaacgccaa gggcaaggtc cgcgagcaat acgctcaggg
    14881 cctggtgggt cgcgacgagc tgctgcaggc cgagtcggct gcgtaccacg gcgccggcac
    14941 ctgcaccttc tatggcaccg ccaacagcaa ccagatgctg ctggaggcga tgggactgca
    15001 cgttccgggt gcagccttcg tgcatccgca cgcagaactg cgcgagaccc tgacgcgcga
    15061 tgcggtgcgc agcgtgctgg gcctggtcaa ggcgcggcgc tacatgccga tcggacaact
    15121 ggtcgacgag cgtgtgatcg tcaacgcgat ggtggcgctg ctggccaccg gagggtcgac
    15181 caaccacctg atccactggg tggccgtggc gcgtgtcgcg ggcatcctga tcgactggac
    15241 cgatttttcc gagctgtccc atgccacgcc gctgctggcg cgcgtgtacc cgaacggcag
    15301 cgcggacgtg aaccagttcc aggcggcggg cggtccgggc tacgtgatcc gggagctgat
    15361 cgatgccggg ctgatgcatg ccgacgtggc gacggtgtcc gatggcggcc tgcgggacta
    15421 cacgcgcatt ccggagcgca ccgaagccgc cgcgctggcg tggcgcgacc tgccgccggc
    15481 gcgcacggca caacacgacg acgacatcgt tcgcccggct gccgcaccgt tcagcgatca
    15541 cggcgggctg gcgctgctca tcggaaacct gggccgggcg gtcatcaaga cctcggcggt
    15601 accggcagac cggcacgcca tcgaggcgcc ggccatcgtg ttcgacacgc aggaggcgct
    15661 gcagacggcg ttcaaggctg gcgaactgga tcgcgacttc gttgcggtgg tgcgtttcca
    15721 gggcccgcgt gccaacggca tgcccgaact gcacaagctg acgccgccgc tggccgttct
    15781 gcagggcaag gggttcaagg tggcgctggt caccgacggt cgcatgagtg gcgcgtcggg
    15841 caaggtgccg gccgccatcc acgtcagtcc cgaggtgctg gccggagggc cgctcggcaa
    15901 ggtgcgcacc ggcgacctga tccggctcga ctccaatgcc ggcacgctgg aggcgctggt
    15961 cgacgcggcc acctgggctg cgcgcagcca ggccgagttg tcggccgagc aggccgaggt
    16021 gaatgcccac ggtctcggac gggaactgtt cggcggcatg cggcggagcg tgctgaccgc
    16081 cgaagagggt gcgatcacct ggctttgagt gccacagttg ccgacccgta cgagaacatt
    16141 ccatccaaga ggtttcatcg atgcaagcgc tcgaactcgc cgccttcggc ccggtcatcc
    16201 cggtcatcgt catccatcgg ttcgaggacg ccgtgcccct ggcgcaggcc ctggtcgatg
    16261 gcggcgtgcg cgtgctggag gtgacgctgc gcacgccggt tgcgctgaaa tgcatggaag
    16321 ccatcgtccg cgcggtgccc ggcgcgatcg tcggggccgg cacggtgcgc tgcgtggccg
    16381 atgtcagggc agcgaaggat gccggctgcc agttcgctgt cagccccggt tacctgagca
    16441 gcatcggtca ggtctgccgc gagatcgacc tgccgttgct gcccggcgtg gcgacctcca
    16501 gcgaggtcat gcaggccaat gccgacggct acgacttcct gaagttcttc ccggccaccg
    16561 cggcaggagg cattccgctg ctgaaggcct tcgccggccc ttttgccgac gtggcgttct
    16621 gcccgacggg cggtatcacg ctcgaatcgg caccgcagtt tctcgcgctg cccaacgtca
    16681 aggtgtgtgg cggctcgtgg ctgacgccgc ccgatgccgt ggcgtccggc gactgggcgc
    16741 gcatcacgcg gctggccgtg gaggcctcgg ctctgcgtgg tgcgtgacgc tgccaaagcg
    16801 cctcgggctc agtcgcggcg ctcgatcagt tcgatcttgt agccgtccgg atcggtcacg
    16861 aaggcgatga cggtggaacc accggcgacc ggaccggctt cgcgcgtcac cgcgccgccg
    16921 ccagtcctga tccgggcgca ggcttcatga gcgtcgggca cggcgatcgc gatgtggccg
    16981 taggccgtgc cgagttcgta atggtcgacg ccgtggttgt aggtcagttc gatctcggcg
    17041 tgctcgggat tcgatccgta gcccacgaag gccaggctgt acttctgctc gggccgttcg
    17101 gtggttcgca acagggtcat gcccatcacg cgggtgtaga agtcgatcga tcgttgcagg
    17161 ttgccgacgc gcagcatggt gtgaagcagt ctcatctggg gttccggttc agggtgggtt
    17221 gtcgttggtc cagagcgtag cgccacaacc gtcgcgacaa tgcgctggtg tttctctgcc
    17281 ggcggccgtc ttgccgcgat gcccgatgac ccgcctcgca ccccgaaccc tgagcctcgg
    17341 cctcgtgctg ctcgccgccg gcggttgctt cgcgctgacg atgggtgtgc gccagtcgat
    17401 gggactgttt ctcggcccga tcaacaccca caccggtctc gggttggcca gcatcagcct
    17461 ggcgttcgcc ttcgggcagc tctggtgggg cctgacacaa ccgttcgccg gcatggtcgc
    17521 cgaccggatc ggcgcaggac gcgtcatcgt ctgcggcgtg ctcatggtgg cggtgggcac
    17581 cgcgttgatt ccgcacatga gcagtgcagc cgggctggtc ttcgcgatcg gtgtgcttgc
    17641 agccggcggc gctggtatag cgggcccttc cgtgctgatg gccgcagcga cacggctgat
    17701 ttcgcctgct cagcggggca tggcaacggg catcgtcaac gccggtggct ccttcgggca
    17761 attcgtcttc gccccgctgg cgcagggtgt caccgtggtg gcggggtgga tgagttcact
    17821 gcatgtgctg ggcgcgctga cgctgctggc gctgcctgca gcctggctgt tgcggggcaa
    17881 cgcactgcac ctggccgcgc cggcgccggg tcagcaggtg gcagagacca cccggcaggc
    17941 gctgtctcga gcgctgggcg atcgcagctt ccgcctgctg tgcgccggct tcttcgtgtg
    18001 cgggtttcac gtcgcgtttc tctcgacaca cctgccaggc gtggtggcgt cctgtcagtt
    18061 gtcgccgaca gtgggtgcct gggcgctggg caccgtcggg ttgttcaaca tcattggcag
    18121 cattggcatc ggctggctga tgtcgtaccg cgccggatac tggcggctga agaccttgct
    18181 gacctggatc tacgccgcgc gcgcagtggc ggttctggtg ttcctgcttt cgccgaagac
    18241 cgaagccgtg atgctggtct tcgctgccgt gatgggcctg acctacctgt cgaccgtacc
    18301 gccgacagtc ggactggtcg ccaagttctt tggcccgtcg cacatggcga ccctctatgg
    18361 cgtggcgatc ctggtccatc agatcggcgc gttcttcggc gcctacctcg gcggcaaggc
    18421 gttcgagtgg accggcagct acgactggat gtggtacgcc gacatcgtgc tcgccgtggg
    18481 cgcggcgctg gtgaacctgc cgatccgcga ggcccgttcg gtgcctctgg gcgcgcaacc
    18541 ggggtgaatc cctccccctt gaacgaaggg ggaacagagc agacatcgcg aattctcgct
    18601 aggcttgatc accctgtgta tcgcagcgcg ctgtcaagag acaactcgaa cggtagcgac
    18661 atgaagattc aatccaaatt caaagcagac gcccgttgtg ccgttgccgt ggcactggcc
    18721 gctggctttt ccgtgtccgc ctgggcgggc gatgacgact ccgagcggtc ttcctgtgca
    18781 ggattgccca cccacgcgca gctcaagacc gagttgacga aggcgcgcaa tgcgtcgaac
    18841 ggcgggttca acctcgacat gtggggcacg gtcgtcaatc gcgacggcgt ggtctgcgct
    18901 gtcgcataca ccggtgccaa tcggggatcg cagtggcccg gcagccgtgc catctcggcg
    18961 cagaaggcca acaccgccaa cgcgttcagc ctgcctggac tcgcgctggc gaccgccaac
    19021 ctttactcgg ccgtccagcc cggcggcagc ctcttcggcc tgcaggaaag caatccggtc
    19081 gacaccgaca acgcctacaa gggccctgcc cagaactggg gtgcgccgaa cgacccgatg
    19141 gtcggcaagc gcgtcggcgg catcaatgtc ttcggtggcg gtctggcgct ctacaacgca
    19201 agcaagcagc tcgtgggcgc gatcggtgtg tcgggtgaca gctcatgtgc cgaccatgcc
    19261 atcgcgtggc gtgcacgcag cacactgtcg ctcgactacg tgccgggtgg tgtcggcccg
    19321 ggcggcgtcg atcagctcag ctacagcgga ccatggtctc agcctcactg tgtgccagga
    19381 ggcgccgtcg ccgaggatgc cgtggtgttg ggtctgccag cgacacgcaa ataacgaatg
    19441 actccaccgg cgtgacgagc cttggatggt tcgtcagccg acgtgttgcg ccccggttgc
    19501 ctgacgtgcc atcggggcgc gtttttttgg ggcttgaatt tgcttccgct ggtcgcgcgc
    19561 gaccggtcac tgagcttgcg agtgcttctt ggtgacgctc agcgtgtctc catcaaccgt
    19621 cacctgggtg ccgactgcca tcgactggga ccgacggaag gtccgcgtgg tgccgtcgtc
    19681 catgcgcacc ttgacgttga agtgggtctc gctgcgcacc tgcttctcga cttcgtggcc
    19741 ggcgacaccg ccgcccaccg cacccagcac ggtcatggcg gtctttccgc tgccgctgcc
    19801 gaactgattg cccaccaccc cgccaagcac gccgcccgtc acggctccga tcccggtgcc
    19861 ctgtcccttc tgcttgacgg ctgtcaccga ttcgacgatg ccgcagttcg ggcacgtgga
    19921 gctggcaggc tgcgccgcat tcgcctctgc gagcatcggc gaaccctggg ctggagggct
    19981 ggaatcaacc gtgcgcgtga tcaacgcacc agccaacccg gccgtgacca gacacagcag
    20041 gccaccgccg atccagacgg cgcgcggcac cgatccggag gaggtcgtct gggaggggac
    20101 gttttgatgg gccgagttca tgtggcagtc cttttcaagg tctttcgaga atcgtaaggc
    20161 agccagtgca ggctgcaagc tgcatttgtt cacgaccgcg aggctttcgc cgccggggag
    20221 tggcggtttt tgatcccgct caaccagggc ctgcggcggt ctgtcgatgc gcgagtcggc
    20281 tgcgggtcat cgttttgcgt ccatccacac cccgaggcct tgcgcgttga gttcgatgtc
    20341 catctcgagc atgtcgagga tctgctttcg ctgcagctgg cattcgagct gtgccacgcg
    20401 ctgcaggtag agcgcgctca gcccgtctcg caggaaggcg tggcgttccc gggcgggcag
    20461 gcccaggccg gcagcctgcc cgcacagagc caccacctcg tcgagctgct cgagcagctc
    20521 gcggcccagt tgctggacat cgctcgggtt ctccgaaccg atgaccggca cccgaccgta
    20581 atgggtcagg tacatgcagg ctggcacctc cgccagcagg cgctcgaccg aggtgcgcaa
    20641 ggctgcgggg tcgaactgca ccggggtcgt ggtcggtagc agccagatcc cggcggacgt
    20701 gtcgaactcc cggtacgaca ggccgaaggt gtcaccggtg aagaagcccc gggagcgttc
    20761 atcccagatg cagtggtgat gacgcgcatg gcccggcgtg tcgagaaacc gcaatggccg
    20821 tcctgccagt cggatcacca ggccgtcgct gctttccgtc acgcggtcgg cggccaccgg
    20881 gaccaccgtg ccgtaggcac gctgcacctc tgcctcgccg tagaccgctc ttgctcctgc
    20941 cagcagcgca tccgggctga tcaggtgccg cgcgccgcgc gggtgcacga cgagccgtgc
    21001 tgaaggcaat gccgccatca gcgcgccggc acctccggca tggtcaagat gcacatgggt
    21061 cggaatcacc caatcgaccg cgtcgcgcgg cacgccggtg gcgtcgagcg ctgccagcag
    21121 ccgtggtacc gagtggctgg ttccgcagtc gatgaacgct gcgcgaccct gttcgacgat
    21181 gaggtaggcc gcgtcgaaat tcgggcgctg gtagccggtg tcgacggccc agatgccctg
    21241 accgagcggg gtggagaatg ggtagattgg ttccatcgcg ctggctcacg gatgcggggt
    21301 agagagtgta ggtttgccga cttcgccgct gcgcagccgg atcaggagta ccgccatgcc
    21361 cgcatacgct cccgtgttgc caaccggagt cctgttgtct cttcgggccc cgcatccgct
    21421 ggcgaagact cgctgccgcc tgccggcgac gctcgcgccc atcatcctcg cgctgctggc
    21481 tggctgttca gcctattcac cctcggcttt gcggcccgga gcgacattgg ccgaggtggt
    21541 ttcggtgatg gggccgccga caggctcggt gcaggcacct cggcggctcg agttcgcgcg
    21601 cggcccttat ggcaagcaca cctacatggc cgatttcgat gccgatggca gactcctttc
    21661 ctgggagcag gtgctcaccg agaacaactt cttccaggtg cagatcggcg aaacgaagga
    21721 agctgtcgtc gagcggctgg gccgcccgtc gtcgaccttt ccgatcggcc gacagcgcat
    21781 cgtcgtctgg aactatcgtt acgaaacccc gttctgccaa tggttccagg tgtccatcgg
    21841 cacggcgccc gcgagcctgg ggcgggtcac cgacgtcggc ttcgggcccg accccatgtg
    21901 cacatcgcgt gaatgaacct cgaccactct cgaccatgac catcatcctg tacggcatcc
    21961 cgaactgcga caccgtgaag aaggcgcgtg tctggctgca ggcgcaaggc ctcgaattca
    22021 ctttccatga tttcaagaag cagggcgtgc cgttgccgcg gctcgacgcc tggctggagt
    22081 cggtcggctg ggagtcgctg ctcaatcgca aggggtccac ctggcgcaag ctggaccccg
    22141 caaagcagga tgccgtgcag gacgccgcca gcgcccgggc gctcatggtg gcgcaggcca
    22201 gcatcatcaa gcgcccggtg gtcgagtact cgggcggcct gctggtcggg ttcgacccac
    22261 aggaatgggg ccgggcgctc gcctgaatcg cgccgagact gttcgggcgc tgtgctgtgc
    22321 tgtgctgtgc tgtgctgtgc tgtgctgtgc tgtgcccgtc aggccgtcag caactgccgc
    22381 agcacgaagg gcaggatgcc gccatgctgg tagtagtcga cctcgatcgg cgtgtcgatg
    22441 cgcagggtca gtggtacacg ccgcttgcgg ccatcggccg acgtgatcac cagcgtcgcg
    22501 tcctgctgcg gttgaatgtg tgccgcaagt tcgatgtcga acagctcgtt gccgcggatg
    22561 cccagcgatt gccagctgtc ggtgcccttg aactgcagcg gcagcacgcc catgccgacc
    22621 aggttgcttc ggtgaatgcg ctcgaagctg cgcgccacca ccgccttgat ccccagcagc
    22681 gccgtgccct tcgccgccca gtcgcgcgac gaaccggtgc cgtactcctc gccggcgaat
    22741 atcagcgtgg gcgtgcccgc tgccatgtac gtcatcgcgg cgtcgtagat gaacgccttc
    22801 tcgccggagg gttgcagcaa ggtgacgcct ccttcctcgc gcgatccgtc cgcgccagct
    22861 ggaatcatca gattcttgat gcgcacgttg gcgaacgtgc ctcgcaccat cacgtcgtgg
    22921 tggccgcggc gtgatccgta gctgttgaag tcggccttca gcacgtggtg ccctgcgagc
    22981 caggcacccg ccggtgagtc ttccttgatc gagccggccg gcgagatgtg gtcggtggtg
    23041 atcgagtcgc cgaaca
//
LOCUS       DAOORV010000052        22491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2798_length_22491_cov_9.371769, whole genome shotgun sequence.
ACCESSION   DAOORV010000052 DAOORV010000000
VERSION     DAOORV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 22491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22491
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_22491_cov_9.371769"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="ilvE"
                     /locus_tag="PKV56_08820"
     CDS             <1..513
                     /gene="ilvE"
                     /locus_tag="PKV56_08820"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOX67957.1"
                     /translation="RGIRVKTSSYTRHHVNITMTQAKAVSNYTNSILANMEATDDGYD
                     EALLLDASGFASEGAGENLFIVKNGVVYTPDLSAGALNGITRNTIFAICSDLGLKIVE
                     KRITRDEIYIADEAFFTGTAAEVTPIRELDRLEIGQGSRGPITEKIQSAFFDIVNGRN
                     PKYAEWLTPV"
     gene            545..748
                     /locus_tag="PKV56_08825"
     CDS             545..748
                     /locus_tag="PKV56_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOX67958.1"
                     /translation="MSAAPSAIEVTAHDLHGPGVVTCPNPKMALWSNHPKVFIDVVTT
                     GSGKCPYCGTVYALKAGEKVHSH"
     gene            761..2347
                     /locus_tag="PKV56_08830"
     CDS             761..2347
                     /locus_tag="PKV56_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX67959.1"
                     /translation="MTASGGGDAGPSDYETLVRAALLGAGTSVWEWNPQTDVISGVDG
                     DLTLLGYTPQESRRTQAAWDRIIHPDYLAENDARYQRHARGETPFYEHEYRALAKDGQ
                     WRWIAERGRVVEWTDEGKPLRVVGTLSDVTQRREAESRVVAMAEHLSQTARHVPGVIY
                     QYRHTGEGHSAFTYVSESCQAVLGVPADELLRDGSKVLQMMEREYLGSTVASMKVSAR
                     TLAPWRFDFRLRRPDGELRWISGASTPERDADGAVIWHGYFEDTTDVHELHLAREAAA
                     MAQAANQAKTEFLSRMSHELRTPLNAVMGFAQLLQLEEDDPLSPNQRRRVALIHEAGG
                     HLLHMIGELLDLTRIESGNLALECVDVRLEPLLVDCVEMLRPSAETADVRLLPIDVPP
                     SLAVRGDPTRLRQVVLNLVSNAIKYNRPGGSAHISASASPDGVVLRVADTGVGIAAEH
                     LLVLGEPFNRLSHQRSVIEGTGIGLAVTRGLIELMAGRLEVQSALGEGSTFSVFLPLA
                     TDVCLEAVARPDDPDVSTVR"
     gene            complement(2397..3110)
                     /locus_tag="PKV56_08835"
     CDS             complement(2397..3110)
                     /locus_tag="PKV56_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOX67960.1"
                     /translation="MDYGRPDLADRLAAEYVTGTLRGPARRRFEQLVPAHPALRAAVR
                     RWESRLMPLTASVSPQAPSAEVWKRIVARIGAAAEPVEATGTPWSSALAFWRSLSMFA
                     GVAALSLLVLFANPQPEQPPIVVVLNSTASPADAAGSTVPATGFVASISGDGRAIVTR
                     PLMSVSVQPDRTLELWAVPGKGAPRSLGLISASGASVVRKNKLPVGTAALAVSLEPPG
                     GSTTGAPTGPVLYVGKLAL"
     gene            complement(3114..3758)
                     /locus_tag="PKV56_08840"
     CDS             complement(3114..3758)
                     /locus_tag="PKV56_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX67961.1"
                     /translation="MSKPPSDWPEKSQELSHLLSRAGLGDRAAFATLYERTSAHLFAV
                     VLRINRDRGQAEDVLQEVYVNVWRSASSFDAAQSQPLTWMTSIARNRAIDGLRRAQTT
                     PRIAQPAQSNLTEDEDSDVYEHMADPAAGPLEMLSQASDARELTHCMDSLSAQQRQSV
                     ALAFYDGLTHAEVASHLGQPLGTVKSWVRRALATLKSCLDNASLRDRRGSETAG"
     gene            3852..4298
                     /locus_tag="PKV56_08845"
     CDS             3852..4298
                     /locus_tag="PKV56_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOX67962.1"
                     /translation="MNTPKAPLMSLSSPDEAELQFYEALQLGDLERFLAVWSDEEDIT
                     CVHPGGPRLIGLPAIRASFGEMFNSGPIDVHAEKVHRLHTHFGAVHSVLERVQVLTEQ
                     GRQSAWVIATNVYLKTALGWRIVAHHASPGTPREAQEILESAAVLH"
     gene            4295..5302
                     /locus_tag="PKV56_08850"
     CDS             4295..5302
                     /locus_tag="PKV56_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX67963.1"
                     /translation="MTVTSFRSPPWLLGTGALGGNLQTIWPALVSKRLDGRAPTYVRH
                     RWTTPDADFIDVDWLAEPPGTHSADRPLLVLFHGLEGSSASHYSRAYAQLAGRLGWRF
                     AVPHFRGCSGEPNLAPRAYHSGDYEEVGWILDRFRRLHTGPRLAVGISLGGNALLRWA
                     EEAGETAANSVRALAAVSSPIDLTAGGHAIGRGFNRQVYTRMFIRSLRPKALLKLAMH
                     PGLFNREKMLCAGDLYDFDNLYTAPVHGFRNTDDYWLRGSAKPHLHRIRIPALVLNAR
                     NDPFVPAASLPRPQDVGDHVTLWNPEHGGHAGFASGGFPGHLQPMHERVAGWLRARAG
                     V"
     gene            5321..5899
                     /locus_tag="PKV56_08855"
     CDS             5321..5899
                     /locus_tag="PKV56_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HOX67964.1"
                     /translation="MDDIVAAALVKWPNVPHCHGWLALDARGDWYLRDERTQAAGPFP
                     MSKGSRLEHVGLREFIERNYACDERGAWFFQNGPQRVYVELEAAPWVWRLHRSSEAAP
                     GPADGVVVRSHSGQAAAFGAAWLDERGRLFIEADLGIGLVHTLDMEIAADAVEQGRWQ
                     VGNVQSGDLPMRFGFRPSPASDVAQAALRLVT"
     gene            complement(5892..6497)
                     /locus_tag="PKV56_08860"
     CDS             complement(5892..6497)
                     /locus_tag="PKV56_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX67965.1"
                     /translation="MRHNSHAHADFARDGTLRTSKQSTGTALLSFILPAIVIVMLAGC
                     GNDNKAPAAGSDGPDAAAVASRIAPVAKVAIKAGSSPSTTPAGAGEAAPAAASAPVAA
                     EAAPVEAPAAAPAAVATAAPAGAAPALYTQACSVCHEAGIAGAPKYGDKAAWAPRLAA
                     GVDGLTASAIKGKNAMPPRGGSQGSDADIKAVVAYMVEAVK"
     gene            6684..6759
                     /locus_tag="PKV56_08865"
     tRNA            6684..6759
                     /locus_tag="PKV56_08865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6717..6719,aa:Arg,seq:ccg)
     gene            complement(6768..7088)
                     /locus_tag="PKV56_08870"
     CDS             complement(6768..7088)
                     /locus_tag="PKV56_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase with chaperone activity"
                     /protein_id="HOX67966.1"
                     /translation="MSDECQTEIPRSFIELFMPPGAVKPQQPRSVITARYDFCEDMAQ
                     MLVDPARDKLFELGVTEQDVLERMLRGLRVDDTAFSAAEATWVVRRLAELLGWPVQTE
                     ATGD"
     gene            7286..7939
                     /locus_tag="PKV56_08875"
     CDS             7286..7939
                     /locus_tag="PKV56_08875"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOX67967.1"
                     /translation="MTSLYSEEHRRLQQEFGTTKLADLLDTGWVHETIADAERDFIGS
                     RDMFFLSTVDPDGNPTVSYKGGPVGVVKVIDEKTLAFPGFDGNGMFYSMGNIAGQARV
                     GLLFIDFESPHRVRVQGTAQLLREHPVMAEYAEAQYLVLITVSKIWVNCPRYIHKYKR
                     VDSSRYVPQPCKTTPLASWKRLDLVQDALSDDDRRRAEQEGVIELPEYEQQVARGEG"
     gene            complement(7976..9070)
                     /gene="ychF"
                     /locus_tag="PKV56_08880"
     CDS             complement(7976..9070)
                     /gene="ychF"
                     /locus_tag="PKV56_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOX67968.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDPRLDALSAIVKPERIVPAIVEFVDIAGLVAGASKGEGLGNQFLAHIRETDALVN
                     VVRCFEDDNVIHVAGKVDPISDIEVIQTELCLADLTTVDKTLQRSIKVAKSGNDKEAA
                     RLVDVLTKCQAALNEAKPVRSIAFSKEELAVLKPLCLITAKPAMFVGNVDEGGFENNP
                     YLDKLRAYADSQKAPVVAICAKTEAELADMADEDRQMFLSEMGQTEPGLNRLIRAAFS
                     LLGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHTDFERGFIRAQTIAFEDFVAFKGE
                     HGAKEAGKMRAEGKEYVVKDGDVLNFLFNV"
     gene            complement(9067..10083)
                     /locus_tag="PKV56_08885"
     CDS             complement(9067..10083)
                     /locus_tag="PKV56_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX67969.1"
                     /translation="MTRFKTWQLFAVCVLSWGTTWHAITYQLGHNTPEVGVALRFGLA
                     SLVVLGLCRARGMNLRFPLRDHALLALQGTLMFGVSYVCVYHAERHLPSGLVAVGYSA
                     APLISGLGAQALFGLRVSRRFIAGGLLGLTGVALMFWPEFGKAAAGMASQAHGAGSLG
                     AAASVGIGAAFTVASVLLSTSGSLTATRNRGRGLPFWPSLGFGMGYGALATTLIAIAM
                     GHSFALPALASWWISLVYLAMAGSVLSFACYLTLQERLGPGPSGAIGVMTPLLALSMS
                     VGFEGYRPDMITGFGVLLALAGNVLMLQRSGTMRPRSAPASDPPRIPASSSTPETPPV
                     RPAP"
     gene            complement(10103..10972)
                     /locus_tag="PKV56_08890"
     CDS             complement(10103..10972)
                     /locus_tag="PKV56_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HOX67970.1"
                     /translation="MSGLPDADVSVRLAALYVHPIKSCGGIAPDDALLIETGLEFDRA
                     WMVVDAQGRFVTQRQVPRMALIQPTLRVLDMVLRAPGMLALHLPLGEVGEAVRVTVWS
                     DEVAAFDMGDLAAQWFSDFLGQPLRVVRFDPDQKRLASAQWTGPIAAEAAFSDEYPLL
                     VASQASLDELNRRLASTGASPVTMQRFRPNLVLDGLDAHGEDHLDEVRFDTPEGPVRL
                     KLVKPCSRCSIPDVDPITAETGHAVADALAAYRADARLNGAITFGMNAVIVEGIECTL
                     RPGMLGGATYRFD"
     gene            complement(10969..13416)
                     /locus_tag="PKV56_08895"
     CDS             complement(10969..13416)
                     /locus_tag="PKV56_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOX67971.1"
                     /translation="MWIRRVALGMLGLLLVLAAVLTWLVMGFDANRYKGIAIEWMQTH
                     HNRTLTINGPIGLSLFPRLQLTLADVSLSEAGRPDEFAGLERAELAVDVLPLLDRELV
                     IGRISARGVRANYLQNALGVRNIDDLLHPEKDPSDPGSIGSKKPPRLDISSIELEDVR
                     LHVKDEVAGVEGDLMVKSLTTGRLRNQAESPVELKLQLDLKAPAVRGELSGDTQLTLD
                     LDTGSVRARHLDLAFKGDTKDISGIRATVKGSAGWDGSTSAIEATALQISVAATAGGL
                     KIDSSSLGIARFAYNPAAKGLELTQLKFEMKGAQEAQSLSFNLDWPELAVAGDQLKGS
                     AFTGRLQRGGAAPLDVNFRSAAPTGSFDLLRLPGVEATFASSGAQRKIGGKLTTNLVL
                     RQGQAAGAGKSTAATPASIALDALTLQSLIENPGLQELKIDARGTASASAQRASWTLA
                     GEMNGAAFNTDGQAQLGGAVPKVIAKARFDTLDLNRLLADAPPPADPTPPPGGKATAA
                     AADTPVDLSGLNGINGQFSLQAGTFAVQRYRVSAARIEATLDRGLLRVTTLQGKIWGG
                     SVDASALAEASTGRLGVKGAATGIDINGALKDVAAKDWMDGTGRVSMDIEATGRSVNE
                     LKSRLQGQLALQLRDGAIKGINLAKSLRQAKAAIGLKQDAVQKASETEKTDFSELSAS
                     FQIADGVARSKDLDVKSPFLRLGGEGAVDIGKGRIDYLARATVTGTSKGQDGADLAAL
                     KGLQVPVRLSGPFEALDWKIEWSSVIAGAVTQQLKNELQNRLEEKLGLKPAEEGASAP
                     AAKPRDALKGLLKGLLK"
     gene            13500..14627
                     /locus_tag="PKV56_08900"
     CDS             13500..14627
                     /locus_tag="PKV56_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOX67972.1"
                     /translation="MKPYDVQVRGTGLVGHALALSLARIGLRVALVADPSASNHVARG
                     PDVRAYALNAGSVGLLRGLKVWDALPPHAATAVHEMRVQGDTGAAVEFSSWEQQVGEL
                     AWIADAAVLERELASAVRFSPHITVVGAGDPGAATAALTAICEGKASASREAHRVPLD
                     RRPYGHRAIATRLTSSKPHAGTARQWFRSPDVLALLPFDAPLPEASYALVWSVPDARA
                     DELLAMDAVDFIDALMQATDGAAGHLDLAGERAAWPLMRAQAGAWCGPGWVLVGDAAH
                     VVHPLAGQGLNLGLADVAALTGVLATREPWRDLGDDKLLRRYARQRLSPTRAMIEITD
                     GLLHLFAHQSPAVRELRNRGLSLVNRLPPIKRWLTARALDS"
     gene            14765..15454
                     /locus_tag="PKV56_08905"
     CDS             14765..15454
                     /locus_tag="PKV56_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOX67973.1"
                     /translation="MGLVPAAHAGEAEIRKALGERLPDLGKVDEVTKIPVAGLFEVRI
                     GTDIYYTDENGSYLFEGHLIDTRNQVNLTEERISKLTAIDFATLPLKDAIVWKQGDGS
                     RKIAVFADPNCGYCKKFERDIRQIKDLTIYTFLFPILGGDSPEKSRNIWCAKDRAKTW
                     VDWMVDGATPPRMMGDCDASALQRNAALGRKHKVTGTPALVFEDGKRVPGAMKADQIE
                     KQLLASAKKPG"
     gene            15451..16716
                     /locus_tag="PKV56_08910"
     CDS             15451..16716
                     /locus_tag="PKV56_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HOX67974.1"
                     /translation="MIPLLQDFLAFEPTYAEALRRLDAVHPGEYARTRNLLDGAVTGL
                     SPYLTHGVISLRAAAARVLQKHRLGYDDKLMFEFAWREFFHHVWSRCGEQVLADMRPA
                     GLWPGSYATTVPADLREGRTGVPTIDAAVRQLYATGYLHNHARMWLASYAVHLRKVHW
                     RAGADWMVGHLLDGDVPSNHLSWQWVAGTFSNKPYLFNADNVARYATGPAFAGWQGRG
                     TVIDQDYEALDRTARLDGDVGPERGEHAGIAEPLLWAAPLPAWVADSTKPLIAAALPK
                     RREIELVTPWSLSEREGGSGAGAGGPWRLGLIHLPAHEAMPWSEARWRWVLRRMGQVT
                     DAVFVGDVMKRPASTQGVPAWPGRDGVIAFRAPMPGYDAALGTLATLRPASRLLPNPE
                     TPCRSFSVFYEAVKRQSRSLQSQLNGELP"
     gene            16770..17177
                     /locus_tag="PKV56_08915"
     CDS             16770..17177
                     /locus_tag="PKV56_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HOX67975.1"
                     /translation="MTLRLGYAGLVPFMLGALLLWLVRPDAHPYVAAALSAYAAVIVS
                     FLGGIHWGLGFRAQPADPSRFIWGVVPSLVAWVAVVMPPYAGLVVHGVMLIVCYGVDR
                     RIYPVQAAAAWLTLRFRLTLVASLSCFIGAAGS"
     gene            17347..19134
                     /locus_tag="PKV56_08920"
     CDS             17347..19134
                     /locus_tag="PKV56_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOX67976.1"
                     /translation="MISYRIDVHNAQAHLFRVTLTIPAPGSTQRLSLPVWIPGSYLVR
                     EFARHLSALEARQGSRPVALTQLDKTTWQAECSGRAALVVTYLVYAFDTSVRAAFLDG
                     HRGFFNGTSLCLRVEGRESGPHRIELKSLPRGWQIATSMASRSAAVFEAVDYDDLVDH
                     PFELGTFWRGRFDAHGVPHELVVAGALPDFDGERLLADTKRICEAEIAFWHGEAAKRA
                     DIPFGRYVLLLNSVDDGYGGLEHRASTALIAARRDLPRLAAPGASTGAKPEESTDGYV
                     TLLGLISHEYFHTWNVKRLRPRDFAPYDYTRENYTELLWFFEGFTSYYDDLFLRRAGL
                     IDGPRYLKAVGKTIGSVLGMPGRQVQSVAQASFDAWVKYYRGDENTPNATISYYTKGS
                     LVALALDLSLRSTKSRRKATLDDVMRALWVRSAGGPIGEADIVAVLREVAGPARAEAL
                     TRQLGEWVHGISDLPLPELLKRFGVQWQSQPATLAQRLGVRVSESALGGVKVTHVLRG
                     GAAERAGVSAGDEVLALGDWRLRRLDDALRLLMPGKPASLLVSRDQRLLTLTVDVPLS
                     ADAVGAVSLGIDARAAHDAIALRTAWILG"
     gene            19176..20330
                     /locus_tag="PKV56_08925"
     CDS             19176..20330
                     /locus_tag="PKV56_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOX67977.1"
                     /translation="MVTAGRVALVASVVAMHACVMHGINQQMLDFETASQMPVRIELV
                     YGRDMESTVPPAPVAAPAVPVAAPPPRPERQATPVADAASAPAEPLEAPMAVADAPLP
                     AASDAAPMVPDATASIAASAPGLASVIGPTSPAASSPADSASGLAAAAPAASAAAETY
                     SWPPSTRITYELTGNYRGEVYGSATVDWVREGARYQVHLDVLVGPAFAPILSRRMTSE
                     GQLTDAGLYPERYDEDSKAMFRDRRRAQVMLEPDAVVLNSGQRVERLAGVQDTASQFV
                     QLAYLFSRHPEMLKPGGTVEMPLALPRRMSVWTYDVREAEVLYTPFGAIDAVPLQPRR
                     ESRKKDELSAQVWFAPSLRYLPVRIRIQQDTNTDTYVDLMIERRPQIAGP"
     gene            20370..21158
                     /locus_tag="PKV56_08930"
     CDS             20370..21158
                     /locus_tag="PKV56_08930"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOX67978.1"
                     /translation="MTHEFILVDTCGDGPLKTGRITLNRPKQLNALNDGLMSELGAAL
                     QAFDADAGIGCIVITGSEKAFAAGADIGAMATLNYMDAYKGDFITRNWEAIRSIRKPV
                     VAAVSGFALGGGCELAMMCDFIIAADTARFGQPEIKLGIIPGAGGTQRLPRAIGKAKA
                     MDLVLTGRMMDASEAERCGLVSRVVPAADLMTVALEAAQNICDYSGPSVMMAKECVNR
                     AFEGTLADGISFERRLFHSLFATEDQKEGMDAFVNKRKPDFKHR"
     gene            complement(21177..21740)
                     /locus_tag="PKV56_08935"
     CDS             complement(21177..21740)
                     /locus_tag="PKV56_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOX67979.1"
                     /translation="MNQRLSTLEEVEAAVWDELQRCVRARGHAWRTCVLASMAPDQAG
                     QPVADARLVVLREADTVARQLRIYTDSRARKVSQLRSNPVATLVMWSAELGWQLRCSV
                     VCSVEDDGLAVSSRWTHIRLSPGAQDYLSPTAPGTPLAGHTPAAAQREHFALITATIT
                     CIDWMELNADGHRRAVFGDGHARWVQP"
     gene            complement(21743..>22491)
                     /locus_tag="PKV56_08940"
     CDS             complement(21743..>22491)
                     /locus_tag="PKV56_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOX67980.1"
                     /translation="EINRNFRNEGISVRHNPEFTMMEFYAAYWNHHDLMDYTEQVLRH
                     AARGATGSATLTYAGRTVDLDAPFTRMTVRESLVAHAGLTFEEAGDAKVLHDKLKSLG
                     EEPPAHWKLPELQFGLFEAVVEEKLWNPTFIIDYPVEVSPLARASDTNPAITERFELF
                     ITGREYANGFSELNDAEDQAARFHAQAANKEAGDEEAMYYDADFIRALEYGMPPTGGC
                     GIGIDRLMMLITDSPSIRDVILFPSLRKEA"
BASE COUNT         3529 a         7560 c         7595 g         3807 t
ORIGIN      
        1 cgcggcatcc gcgtcaagac cagcagctac acgcgccacc acgtcaacat cacgatgacg
       61 caggccaagg cggtcagcaa ctacaccaac tcgatcctcg ccaacatgga agccaccgac
      121 gatggctatg acgaggcgct gctgctcgat gccagtggct tcgcgagcga gggggccggc
      181 gagaacctgt tcatcgtcaa gaacggcgtg gtctacaccc ccgacctgtc tgccggggca
      241 ttgaacggca tcacgcgcaa caccatcttc gcgatctgct cggacctcgg cctgaagatc
      301 gtcgaaaagc gcatcacgcg cgacgagatc tacatcgccg atgaagcctt cttcaccggc
      361 accgcggccg aggtgacgcc gatccgcgaa cttgatcgac tcgagatcgg acagggatcc
      421 cgcgggccga tcaccgagaa gatccagagc gcgttcttcg acatcgtgaa cggccgcaat
      481 ccgaagtacg ccgagtggct gacaccggtc tgaagcacgt tccaactttc tcgacggagc
      541 ccacatgagc gcagccccct ctgccatcga agtcaccgca cacgatctgc acggcccggg
      601 tgtcgtcacc tgccccaacc cgaagatggc gctgtggagc aaccacccga aggtcttcat
      661 tgacgtggtc accaccggtt cgggcaagtg cccttattgc ggcacggtgt atgcgctcaa
      721 ggcgggtgag aaggttcatt cgcactgagt gcgccgctcc atgaccgcat ccggaggcgg
      781 cgacgcgggt ccgagtgact acgagacgct ggtccgcgcg gcgctgctcg gtgccggtac
      841 ttccgtctgg gagtggaatc cgcagaccga tgtcatctcg ggcgtcgatg gcgacctgac
      901 actgctcggc tacacgccgc aagagagtcg tcgcacgcag gccgcctggg accgcatcat
      961 ccacccggac tatctcgccg agaacgacgc tcggtaccag cgccatgcgc gcggcgagac
     1021 accgttctac gagcacgagt accgcgcgct ggccaaggat ggtcagtggc gctggattgc
     1081 ggagcgcggc cgcgtcgtcg aatggaccga cgaaggcaag ccgttgcgag tcgtgggcac
     1141 gctgagcgat gtgacccagc ggcgggaggc cgagagccgc gtggtcgcga tggccgagca
     1201 cctcagccag accgcgcgtc acgtgcccgg ggtcatctac cagtaccgcc acaccggcga
     1261 aggccacagc gcctttacct acgtcagtga aagttgtcag gcggtgctgg gggtgccggc
     1321 cgacgagttg cttcgcgatg ggtccaaggt gttgcagatg atggagcgcg agtacctcgg
     1381 ctcgaccgtg gccagcatga aagtctcggc acgcacgctg gctccgtggc gattcgattt
     1441 ccgcctgcgg cgcccggatg gcgagttgcg ctggatctcc ggagcgtcga cccccgagcg
     1501 cgatgccgac ggcgccgtca tctggcatgg ctatttcgag gacaccaccg atgtgcatga
     1561 actgcacctc gcccgcgaag ctgccgccat ggcacaggcc gccaaccagg ccaagaccga
     1621 attcctctcg cgcatgagcc acgagttgcg tacgccgttg aatgcggtga tgggtttcgc
     1681 gcaactgctg caactcgagg aggacgaccc gctttcaccg aaccagcgcc ggcgggtggc
     1741 gctgatccac gaggcgggcg gccacctgct gcacatgatc ggcgagctgc tcgacctcac
     1801 gcgcatcgaa tcgggcaacc tcgcactgga gtgcgtcgat gtcaggctgg aaccactgct
     1861 cgtcgattgc gtcgagatgc tgcggccatc ggccgaaacg gccgatgttc gtctgctgcc
     1921 gatcgatgtt ccgccgagcc tcgcggtgcg cggggacccg acgcgactgc gtcaggtggt
     1981 gctgaacctg gtcagcaacg cgatcaagta caacaggccg ggtggatctg cgcacatcag
     2041 tgccagcgcg tcgccggatg gcgtggtgct gcgcgtcgcc gacaccggag tcggcattgc
     2101 agccgaacac ctgctcgtgc tcggtgaacc tttcaatcgc ctgtcgcacc agcgcagcgt
     2161 gatcgaaggc acgggcatcg ggctcgcggt cacccgcggg ctgatcgagc tgatggccgg
     2221 ccgcctcgaa gtgcagagcg ccttgggcga gggctcgacc ttcagcgtgt tcctgccctt
     2281 ggccaccgac gtgtgcctgg aagcggtcgc tcgaccggac gatcccgacg tttcgacggt
     2341 gcgctgacgc agcaggggcc cgcgagccct ttcggcgaat tccggttggg cagggtctac
     2401 agggccagct tgccgacgta caggactggt ccggtcgggg cgcccgtcgt cgaaccgcca
     2461 ggcggctcca gactgacggc cagcgctgcg gtaccgacgg gcagcttgtt cttcctgacg
     2521 acgctggccc cggatgccga gatcaggccg agcgagcgcg gcgcaccctt gcccggcacc
     2581 gcccacagct cgagtgtgcg atcaggctgc acgctgacgc tcatcagcgg ccgcgtgacg
     2641 atggcccggc cgtcgccgct gatgctggcg acaaagccgg tcgcaggcac cgtcgaaccc
     2701 gcggcatcgg ctggcgacgc ggtcgagttc agcaccacga cgatcggcgg ctgctcgggc
     2761 tgcgggttgg cgaacaggac cagcaggctc aaggcggcga cgccggcgaa catggacagg
     2821 ctgcgccaga acgccagcgc cgacgaccat ggtgtgccgg tcgcctccac aggttcagcc
     2881 gcggcgccga tgcgcgcgac gatgcgtttc cagacttctg cggaaggtgc ctgcggagac
     2941 acgctggcgg tcagtggcat caagcgggac tcccatcgcc tgactgcagc acgcagcgca
     3001 ggatgggccg gcaccagctg ctcgaagcgt cgccgtgccg ggccgcgcag cgtgcccgtc
     3061 acgtactcgg cggcgagtcg atcggcgaga tcaggtcgtc cgtagtccat gggtcagccc
     3121 gcagtctcgc tacctcgacg gtcgcgcagg ctggcgttgt cgaggcagct cttcaaggtg
     3181 gcgagcgcgc gccggaccca cgatttcacc gtgcccagtg gctggccgag atggctggcc
     3241 acctcggcgt gcgtcagtcc atcgtagaag gcgagtgcca cgctttgccg ctgctgcgca
     3301 ctcaaggagt ccatgcagtg ggtcagctcg cgcgcatccg aagcctggct cagcatctcg
     3361 agcggtcccg ccgcggggtc ggccatgtgt tcatagacat cgctgtcttc gtcctcggtg
     3421 aggttggact gtgcgggctg cgcgatcctc ggcgtggtct gtgcgcgtcg caagccatcg
     3481 atggcgcgat tcctggcgat gctggtcatc caggtcagcg gctggctctg cgccgcgtcg
     3541 aacgaactcg ccgagcgcca gacgttgaca tagacctcct gcagcacatc ctccgcctga
     3601 ccccgatccc tgttgatacg cagcaccact gcgaacagat gcgcgctggt gcgttcgtag
     3661 agtgtggcaa atgccgcacg atcacccaga cccgcgcgcg acagcaggtg agacagctcc
     3721 tggctctttt caggccagtc ggaagggggc ttggacatgg gctcgggtcg tccgggtggc
     3781 ttcgtacaat ggcaggtcgc gcggccgacg ctaatctttc actacgctcg ccgcaacaga
     3841 tgaaaggtcg aatgaacact cccaaggctc cgctgatgtc gctgtcatca cccgatgagg
     3901 cggagctgca gttctacgaa gcgctgcagt tgggggatct cgagaggttt ctggcagtct
     3961 ggagcgacga ggaagatatc acctgcgtgc accccggcgg gccgcggctc atcgggctgc
     4021 cggcgatccg ggcgagtttc ggcgagatgt tcaacagcgg tccgatcgat gtccacgccg
     4081 aaaaggtcca ccggctgcac acccacttcg gcgccgtaca cagcgtgctg gagcgtgtgc
     4141 aggtgctgac cgagcaggga cggcagtccg cctgggtcat cgccaccaac gtctacctga
     4201 agaccgctct gggctggcgc atcgttgctc accacgcgag tccgggtacg ccgcgcgagg
     4261 cgcaggagat cctcgaatcg gcggccgtgc tgcattgacc gtcacctcgt ttcgatcgcc
     4321 gccctggctg ctcgggacgg gcgcgctggg tggcaacctg cagacgatct ggcctgcgct
     4381 ggtgtcgaag cgtctcgacg gccgcgcgcc gacgtacgtc cggcaccgct ggaccacgcc
     4441 cgatgcggac ttcatcgatg tcgactggct ggctgaaccg cctggcacgc actcggccga
     4501 ccgaccgctg ctggtgctgt tccacgggct cgaaggctcg tcggccagcc actactcgcg
     4561 tgcctatgcg caactggccg ggcgtctcgg ctggcggttt gccgtgccgc atttccgagg
     4621 ctgttcgggc gagcccaatc tcgcgccccg cgcctaccac tcgggcgatt acgaggaagt
     4681 cggctggatc ctcgatcggt ttcgcaggtt gcacaccggc ccgcgtctgg cggttggtat
     4741 ctccctcggc ggcaacgccc tgctgcgctg ggccgaggag gccggtgaga cggcggcgaa
     4801 cagcgtgcgt gcgttggctg cggtctcctc tccgatcgac ctgaccgcgg gcggccatgc
     4861 catcggacgc gggttcaatc ggcaggtcta cacgcgcatg ttcatacgct cgctgcgacc
     4921 gaaggcgctg ttgaagctgg ccatgcaccc cgggctcttc aatcgcgaga agatgctttg
     4981 cgcaggcgac ctgtacgact tcgacaacct ctacaccgcg cccgtccacg gttttcgcaa
     5041 caccgacgac tactggcttc gcggatcggc caaaccacac ctgcaccgca tccgcatccc
     5101 ggcgctggtg ctgaatgcgc gcaacgaccc gttcgtgccg gccgcgtcgc tgccccgccc
     5161 gcaggacgtt ggcgaccacg tgaccttgtg gaaccccgag catggtgggc atgccggctt
     5221 tgcgagtggc gggtttcccg ggcacctgca gccgatgcac gagcgcgtgg cgggctggct
     5281 gcgcgcgcgc gccggagtct gagcacccgg ggcgcctggc atggatgaca tcgtcgctgc
     5341 ggcgctcgtc aagtggccga acgtgcccca ctgccacggc tggcttgcgc tcgacgcacg
     5401 cggcgactgg tacctgcgcg acgaacgcac ccaggcggcg gggccgttcc cgatgtccaa
     5461 gggcagccgg ctcgagcacg tcgggctgcg cgagttcatc gagcgcaact acgcctgcga
     5521 tgagcgtggc gcctggttct ttcagaacgg gccgcagcgg gtctatgtcg aactggaagc
     5581 ggcgccctgg gtctggcggt tgcaccggtc gtcggaagcc gcccctggcc cggccgacgg
     5641 ggtcgtcgtg cgcagccaca gcggccaggc ggcagctttc ggtgccgcct ggctcgatga
     5701 acgcggccgg cttttcatcg aggccgatct cggcatcggg ctggtgcaca cgctcgacat
     5761 ggagatcgcc gccgatgcgg tcgagcaggg ccggtggcag gtcggaaatg tgcagtcagg
     5821 cgacctgccg atgcgcttcg gcttcaggcc gagcccggcg tccgatgtcg cacaggccgc
     5881 cttgcgcctg gttacttgac ggcttcgacc atgtaggcca cgacggcctt gatgtccgcg
     5941 tcgctgccct ggctgcctcc gcgtggcggc atggcgttct tgcccttgat cgcgctggcg
     6001 gtcaggccat cgaccccggc ggccaagcgc ggcgcccagg ccgccttgtc gccgtacttc
     6061 ggcgcaccag caatgcccgc ttcgtgacag accgagcagg cttgcgtgta gagggccggc
     6121 gccgcgccgg caggggccgc agtggccacc gcagcgggtg cggctgcagg cgcctcgacc
     6181 ggggcagctt cggccgcgac gggtgccgat gctgctgcag gcgccgcttc cccagcacct
     6241 gccggcgtcg tggacgggct cgaccccgcc ttgatcgcaa ccttggccac aggcgcgatg
     6301 cggctggcta ccgccgcagc atccgggccg tcgctgccgg ctgccggcgc cttgttgtcg
     6361 ttcccgcagc cggccagcat cacgatgacg atcgccggga gaatgaacga gaggagggcg
     6421 gtgccggtcg actgcttcga tgttctgagc gtgccgtctc gagcaaagtc ggcgtgggcg
     6481 tggctgttgt ggcgcatgag atcctcggat ggcaggttgg tgctgtattc gaatggaaga
     6541 agagccctgc atcgagggca ctgggacatc tcgcgagccg cgcagacccc ctgccagagg
     6601 gcgcgttagg ccgccaaatc agtatagagc ggggtgccgc tgctcgttcg tcgacgaggc
     6661 gcccgactac aatcgtgggc gttgcgtccg tagctcaatg gatagagtac tgccctccga
     6721 aggcaggggt tgctggttcg atcccagccg ggcgcaccac ctgaacttca atcgccagtg
     6781 gcttcggtct gcacaggcca gcccagcaat tccgccagcc gccgcacgac ccaggtcgcc
     6841 tctgcggcgc tgaacgccgt gtcgtcgacc ctcaggcccc gcagcatccg ctcgagcaca
     6901 tcctgttcgg tcacacccag ttcgaacagc ttgtcgcggg ccgggtccac cagcatctgt
     6961 gccatgtctt cgcagaagtc atagcgggcg gtgatcaccg atcgaggctg ctgcggcttc
     7021 accgcacccg gcggcatgaa caactcgatg aacgagcgcg ggatctcggt ttgacattcg
     7081 tcgctcatgg cgcatccgat cgcagtcgat gatttcaatc acgcagattg tggtgagctg
     7141 cagagcatag gcgccagccg gtcggcgatc agggtctgtc gcgatgcaag acggacattg
     7201 ccgctgctac cgtcgcggcg ccgaggcgct tggcggcgag cgatccggct cgtgccggtc
     7261 tgcggctgac acccagggag acgccatgac ctcgctttat tccgaagagc accgacgcct
     7321 gcagcaggaa ttcggaacca ccaagctggc cgacctgctc gataccggct gggtgcacga
     7381 gacgatcgcc gatgccgagc gcgatttcat cggttcgcgc gacatgttct tcctgtccac
     7441 cgtcgacccg gacggcaatc cgaccgtctc gtacaagggc ggcccggtcg gcgtggtgaa
     7501 ggtgatcgac gagaagacgc tggcgtttcc ggggttcgac ggcaacggca tgttctattc
     7561 gatgggcaac atcgccgggc aggcccgggt cggcctgctg ttcatcgatt tcgagagtcc
     7621 gcaccgcgtg cgcgtgcagg gcacggcgca gttgctgcgc gaacatccgg tgatggccga
     7681 gtacgccgag gcgcagtacc tggtgctgat cacggtcagc aagatctggg tcaattgccc
     7741 gcgctacatc cacaagtaca agcgcgtcga ctcgtcgagg tacgtgccgc agccctgcaa
     7801 gaccacgccg ctggcctcgt ggaagcggct ggatctggtg caggacgcgc tcagcgacga
     7861 cgatcgccgg cgtgccgagc aggagggagt gatcgagctg ccggaatacg agcagcaggt
     7921 cgcgcgcggc gaagggtgac gtcgaggggc ggcaggccct gcctgccgcc agggctcaga
     7981 cgttgaacag gaagttcagc acatccccgt ccttgaccac gtattccttg ccttcggcgc
     8041 gcatctttcc ggcttccttc gcgccgtgtt cgcccttgaa ggcgacaaag tcctcgaagg
     8101 cgatcgtctg ggcgcggatg aatccgcgct cgaagtcggt gtggatcacg ccggcggcct
     8161 gcggcgcggt gtcgccgatg tggatggtcc aggcgcgcac ttccttcacg ccggcggtga
     8221 agtaggtctg caggcccagc aggctgaacg cagcgcggat cagccggttc aggccgggct
     8281 cggtctggcc catctccgac aggaacatct gccggtcctc gtcggccatg tccgcgagtt
     8341 cggcctcggt cttggcgcag atcgccacga ccggggcctt ctggctgtcg gcataggcgc
     8401 gcagcttgtc gaggtagggg ttgttctcga agccgccctc gtcgacgttg ccgacgaaca
     8461 tcgcgggctt cgcggtgatc aggcacagcg gcttcagcac cgccagttct tccttgctga
     8521 aggcgatgct gcgcaccggc ttcgcctcgt tcagcgcggc ctggcacttg gtcagcacgt
     8581 cgaccaggcg agcagcttcc ttgtcgttgc ccgacttcgc gaccttgatg gagcgctgca
     8641 gcgtcttgtc cacggtggtc aggtcggcca ggcacagctc ggtctggatc acctcgatgt
     8701 cggagatcgg gtcgaccttg ccggccacgt ggatcacgtt gtcgtcctcg aagcagcgca
     8761 ccacgttgac cagcgcatcg gtctcgcgaa tgtgcgccag gaactggttg cccaggcctt
     8821 cgcccttcga ggcaccggcc accagtccgg cgatgtcgac gaactcgacg atcgccggca
     8881 cgatgcgctc gggcttcacg atggccgaca gtgcatcgag ccgcgggtcg ggcagctcga
     8941 cgatgccgac gttgggctcg atggtgcaga acggatagtt ctcggcggcg atgccggcct
     9001 tggtgagcgc gttgaagagg gtggatttgc cgacgttggg caaaccgacg atgccgcatt
     9061 tcaggctcat ggtgcaggtc tcacaggagg tgtttcgggc gtcgacgacg aggcggggat
     9121 tctaggaggg tcgctggcag gggcagatcg agggcgcatc gtgccgctgc gctgcagcat
     9181 cagcacgttg ccagccaacg ccagcagcac gccgaatccg gtgatcatgt cgggccggta
     9241 gccctcgaac ccgaccgaca tcgacagcgc cagcagcggc gtcatcacgc cgatcgcgcc
     9301 ggacggcccg gggcccaggc gctcctgcag cgtcaggtag caggcgaacg acagcaccga
     9361 gccggccatc gccaggtaca ccagcgagat ccaccacgag gccagagccg gcagtgcgaa
     9421 gctgtggccc atcgcgatcg cgatcagcgt cgttgccagc gcgccgtagc ccatgccgaa
     9481 gccgagcgag ggccagaaag gcaggccgcg accgcggttg cgcgtcgcgg tcaggctgcc
     9541 cgacgtgctc agcagcaccg atgcgacggt gaacgccgcg ccgatgccca ccgacgctgc
     9601 cgcgccgagc gatcccgcgc cgtgcgcctg agatgccatg ccggccgcgg ccttgccgaa
     9661 ttcgggccag aacatcagag ccaccccggt caggcccagc agcccgcccg cgatgaagcg
     9721 gcgcgaaacg cgcagcccga acagcgcctg cgcacccagc ccgctgatca gcggcgctgc
     9781 cgagtagccg accgccacca ggcccgacgg cagatgccgc tcggcgtgat acacgcagac
     9841 gtacgacacg ccgaacatca gcgtgccctg gagcgccagc agcgcgtggt cgcgcagtgg
     9901 gaaccgcagg ttcatcccgc gagccctgca cagcccgaga acgaccagcg aagccagccc
     9961 gaagcgcagt gcgacgccga cctcaggcgt gttgtggccc agctggtagg tgatcgcgtg
    10021 ccacgtcgtg ccccagctca gcacgcagac cgcgaagagc tgccaggtct tgaaacgggt
    10081 catgaaaggc gcgcggcccg cttcaatcga agcggtacgt cgcgccgccg agcatgcccg
    10141 gccgcaaggt gcattcgatg ccctcgacga tgactgcgtt cattccgaag gtgatcgctc
    10201 cgttgagccg tgcatcggcc cggtaggcag cgagcgcgtc ggccaccgcg tggccggtct
    10261 cggcggtgat cgggtcgaca tcggggatgc tgcagcgcga gcagggcttg accagcttca
    10321 gccgcaccgg tccctcgggc gtgtcgaagc gcacctcgtc gaggtggtcc tcgccgtgcg
    10381 catcgagccc gtcgagtacc aggttcgggc ggaagcgctg catcgtcacc ggcgaggcgc
    10441 ccgtggacgc gagccgtcga ttcagctcgt cgaggctggc ctgcgaggcg accagcagcg
    10501 ggtactcgtc ggagaacgcc gcctcggcag cgatgggccc ggtccactgt gcgctggcga
    10561 gccgcttctg gtctgggtcg aagcgcacga cgcgcaaggg ctggccgagg aagtcgctga
    10621 accattgcgc ggccaggtcg cccatgtcga acgcggcgac ttcgtcgctc cacacggtca
    10681 cgcgcaccgc ttcaccgacc tcgcccagag gcaggtgcag cgccagcatg cccggtgcgc
    10741 gcagcaccat gtcgagcacc cgcagcgtcg gctggatcag cgccatgcgt ggcacctggc
    10801 gctgggtgac gaagcggccc tgcgcatcga cgaccatcca ggcccggtcg aactcgagcc
    10861 cggtctcgat cagcagcgcg tcgtcgggcg cgatgccgcc gcagctcttg atcgggtgca
    10921 catacagcgc ggcgagccgc accgacacgt cggcgtccgg caggccgctc acttcagcag
    10981 ccctttcagc aggcccttca gggcatccct gggcttggcc gccggggccg aagcgccctc
    11041 ctcggcgggc ttcaggccga gcttttcctc cagccggttc tgcaactcgt tcttcagctg
    11101 ctgcgtcacc gccccggcga tcaccgacga ccactcgatc ttccagtcca gcgcctcgaa
    11161 cggcccgctc agtcgcaccg gcacctgcag gcccttcagc gcggccaggt cggcgccgtc
    11221 ctgacccttc gaggtgccgg tcacggtggc ccgcgccagg tagtcgatgc ggcccttgcc
    11281 gatatcgacc gcgccctcgc cacccaggcg caggaacgga ctcttcacgt ccagatcctt
    11341 gctgcgggcg acgccgtccg cgatctggaa gctcgcgctc agttcgctga aatcggtctt
    11401 ctcggtctcg ctggccttct gcaccgcgtc ctgcttgagt ccgatggccg ccttggcctg
    11461 gcgcaggctc tttgcgaggt tgatgccctt gatcgcgccg tcgcgcagtt gcagcgcaag
    11521 ctgcccttgc agccgggatt tcagctcgtt gacgctgcgg ccggtggcct cgatgtccat
    11581 cgacacgcgg ccggtgccgt ccatccagtc cttcgccgcc acgtccttca gcgcgccgtt
    11641 gatgtcgatg ccggtggctg cgcccttgac gccgaggcgc ccggtcgatg cttcggccag
    11701 cgccgatgca tcgacgctgc cgccccagat cttcccctgc agcgtggtca cgcgcagcag
    11761 accccggtcc agcgtggctt cgatgcgggc tgccgaaaca cggtaccgct gcaccgcgaa
    11821 ggtgcccgcc tgcaggctga actgtccgtt gatgccgttc agtccgctca ggtcgaccgg
    11881 ggtgtcggcc gcagccgcag tcgccttgcc cccgggcggc ggcgtggggt cggccggtgg
    11941 aggcgcatcg gccagcaggc ggttcaggtc cagcgtgtcg aagcgtgcct tggcgatcac
    12001 cttcggcacg gcaccgccga gctgggcctg cccgtcggtg ttgaacgccg cgccgttcat
    12061 ctccccggcc agcgtccagc tggcgcgctg cgccgacgcg ctggccgtgc cgcgcgcgtc
    12121 gatcttcagt tcttgcaggc ccgggttctc gatcaggctc tgcaatgtca gcgcatcgag
    12181 cgcgatcgat gcgggcgttg cggcggtgct cttgccggct ccggcggctt gcccctgtcg
    12241 caagacaagg tttgttgtca gcttgccccc gatcttgcgc tgggcgccgc tgctggcgaa
    12301 ggtggcctcg acgccgggaa gccgcagcag atcgaaactg ccggtcgggg ccgcactcct
    12361 gaaattcacg tcgagcggtg ccgcgccgcc gcgctgcagt cgcccggtga aggcgctgcc
    12421 cttgagctga tcaccggcca cggccagttc gggccagtcc aggttgaagg acaaggactg
    12481 ggcttcctgg gcccccttca tctcgaattt cagctgagtg agctccaacc ccttggccgc
    12541 cgggttgtag gcgaagcgcg cgatgcccag cgagctgctg tcgatcttca ggccgcccgc
    12601 ggtcgcggcc acgctgatct gcagcgccgt tgcctcgatc gccgaggtgg agccgtccca
    12661 gcccgcgctg cctttcacgg tggcgcggat cccgctgatg tccttcgtgt cgcccttgaa
    12721 cgcgaggtcg agatggcgtg cgcgcaccga gccggtgtcc agatcgagcg tcagttgtgt
    12781 atctccgctc agttcgcccc gcacggcggg cgccttcagg tctaactgca atttcagctc
    12841 gaccggcgac tcggcctggt tgcgcaggcg accggtggtg agcgacttca ccatcagatc
    12901 gccctcgacg ccggccactt cgtccttgac gtgcaggcgc acgtcttcca gttcgatgct
    12961 gctgatgtcg agccggggcg gcttcttgct gccgatgctc ccggggtccg aagggtcctt
    13021 ctcggggtgc agcagatcat cgatgttgcg cacgccgaga gcgttctgca ggtagttggc
    13081 gcgtactccg cgcgccgaaa tccgtccgat caccagctcg cggtcgagca ggggcagcac
    13141 atcgacggcg agttcggcgc gttcgagccc ggcaaactcg tcgggccgcc cggcctcgct
    13201 cagggaaacg tcggccagcg tgagctgcag gcgcgggaaa agcgacaagc cgattggccc
    13261 gttgatcgtc agcgtgcggt tgtggtgggt ctgcatccac tcgatggcga tgcccttgta
    13321 ccggttggcg tcgaagccca tgaccagcca ggtgagcacc gctgcgagca ccagcagcag
    13381 tcccaacatc ccgagtgcca cacggcgaat ccacatcgac acggcaaagc ccccgaaaac
    13441 ggcggattgt gcctgtcccg ttccggcccg ccagactcgg ctccctacaa tcgaccgcga
    13501 tgaaacccta tgacgtgcag gtgcgtggaa ccggtctggt cggccacgcg ctggccttgt
    13561 cgctggcgcg catcggactg cgtgtggcct tggtcgctga cccgtcggca tcgaaccacg
    13621 tggcgcgcgg ccccgacgtg cgcgcctacg cgctgaatgc aggatccgtc ggcctgctac
    13681 gtgggctcaa ggtgtgggat gcgttgccgc cgcatgcggc cacggccgtg cacgagatgc
    13741 gcgtgcaggg agacaccggc gctgctgtcg agttttcctc gtgggagcag caagtcggtg
    13801 aactcgcctg gatcgccgat gcggccgttc tcgaacgcga actggcttct gcggtgcggt
    13861 tctcgccgca catcaccgtg gtcggggcgg gcgatccggg ggcagcgact gctgcgctca
    13921 ccgccatctg cgaaggcaag gcttccgcca gccgcgaggc gcatcgcgtg ccgctcgacc
    13981 ggcgtcccta cggtcaccgc gcgatagcga cgcgcctgac cagctcgaag ccgcatgcgg
    14041 gtaccgcacg ccagtggttc cgctcgccgg atgtgctggc gctgctgcct ttcgatgcgc
    14101 cgctgccgga ggcctcgtat gcgctggtct ggtccgtgcc cgatgcgcgc gccgacgagt
    14161 tgctcgcgat ggatgccgtc gatttcatcg atgccctgat gcaggccacc gatggcgccg
    14221 ccggtcatct ggacctggca ggcgagcgtg ccgcctggcc gctgatgcgt gcccaggctg
    14281 gcgcctggtg cgggccgggt tgggtgcttg tcggtgatgc cgcgcatgtc gtgcacccgc
    14341 tggcgggcca gggcctgaac ctgggactgg ccgatgtggc ggcgctgacc ggggtcctcg
    14401 ccacgcgcga gccgtggcgt gatctgggcg atgacaagct gctgcgccgc tatgcgcggc
    14461 agcgtttgtc accaacgcgc gcgatgatcg agatcaccga tggactgctg catctttttg
    14521 cgcatcagtc gccggccgtg cgggaactcc gcaaccgcgg gctgagtctg gtcaatcggc
    14581 tcccgcccat caagcgctgg ttgactgcac gggcgctgga ttcgtgaagc cgcctggcct
    14641 ggccctgccc tcttcgatca cccgaaagtc caacatcatg gtcactccct tgccttcctt
    14701 ccgtgcgctg catcgcagct tggtgcacct tgcttcctca ggcgcgctgt cgctgctggt
    14761 gttgatgggc ctcgtgcccg ctgcacatgc cggagaggcg gagatacgca aggcgctggg
    14821 tgaacggctg cccgatctcg gcaaggtcga cgaggtcacg aagatcccgg tcgcaggtct
    14881 gttcgaggtc cgcatcggca ccgacatcta ctacaccgac gagaacggca gctacctgtt
    14941 cgaaggtcat ctgatcgaca ccaggaacca ggtcaacctg accgaggagc gcatctccaa
    15001 gctgaccgcc atcgattttg cgaccctgcc gctgaaggat gcgatcgtct ggaagcaggg
    15061 cgatggcagt cgcaagattg cggtgttcgc cgatccgaac tgcggatact gcaagaagtt
    15121 cgagcgcgac atccgccaga tcaaggacct gacgatctac accttcctgt tcccgatcct
    15181 cggtggcgat tcgcccgaga agtcgcgcaa catctggtgc gcgaaggacc gtgcgaagac
    15241 ctgggtcgac tggatggtcg atggcgcgac cccaccgcgc atgatgggtg actgcgatgc
    15301 ctccgcgctg caacgcaatg cggcgcttgg tcgcaagcac aaggtcaccg gcacgccagc
    15361 gctggtgttc gaggacggca agcgggtgcc cggtgcgatg aaggccgacc agatcgagaa
    15421 gcagttgctg gcctccgcga agaagccggg ttgattccgc tgctgcagga tttcctcgcc
    15481 ttcgagccga cctacgccga ggcgctgcgg cgtctcgacg cggtgcatcc tggcgagtac
    15541 gcgcgcaccc gcaacctgct cgacggggca gtcaccggcc tgtctccgta cctgacgcac
    15601 ggtgtgatct cgctgcgtgc ggcagcggcc cgggtgctgc agaagcaccg gctcgggtat
    15661 gacgacaagc tgatgttcga attcgcgtgg cgcgagttct tccaccacgt ctggagccgc
    15721 tgcggcgagc aggtgctggc cgacatgcgc cctgcgggac tttggccggg cagctacgcc
    15781 accacggtgc cggccgatct gcgcgagggt cgcaccggcg tgccgacgat cgatgcggcg
    15841 gtgcgccagc tctacgccac cggttacctc cacaaccacg cccgcatgtg gctggccagc
    15901 tatgcggtgc acctgcgcaa ggtgcactgg cgtgcgggtg ccgactggat ggtcggccac
    15961 ctcctggacg gtgacgtgcc gagcaaccac ctgagctggc agtgggtggc gggcaccttc
    16021 tcgaacaagc cctacctgtt caacgcggac aacgtggccc ggtacgcgac cggcccggcc
    16081 ttcgcaggct ggcagggcag gggaaccgtg atcgaccagg actacgaggc gctcgaccgc
    16141 accgcacgtc tggacggcga tgtcggcccc gagcgcggcg agcatgccgg catcgcggag
    16201 ccgctgttgt gggccgcgcc tctgcccgca tgggtcgccg actccacgaa gccgctgatc
    16261 gctgccgcac tgccgaagcg gcgcgagatc gaactggtca cgccgtggtc cctgagcgag
    16321 cgtgaaggcg gttcgggcgc cggtgccggt ggcccgtggc gcctgggcct gatccacctg
    16381 ccggcgcatg aagcgatgcc ctggagcgag gcacgctggc gctgggtgct gcgacgcatg
    16441 gggcaggtca ccgatgcggt cttcgtcggc gacgtgatga agaggccggc gtccacccag
    16501 ggcgtgcccg cttggcccgg acgcgacggg gtcattgcct ttcgggcgcc catgcccggc
    16561 tacgacgcgg cgctcggcac gctcgcgacc ttgcgccccg cgtcgcgcct gctgccgaat
    16621 cccgagacgc cctgccgctc gttcagcgtg ttctatgaag cggtcaagcg acaatccagg
    16681 tcgttgcaat cgcagttgaa cggagagctt ccttgagtgg tcggagtcgg tttccggacg
    16741 cacccgcgtc gacgaatcag cccgacgcgg tgaccttgcg tctgggctat gcggggctgg
    16801 tgccgttcat gctgggtgca ctgctgctgt ggctcgtgcg gcccgatgcc cacccttacg
    16861 tcgcggccgc actgtcggcg tatgccgcag tcatcgtctc gtttctcggc ggcatccact
    16921 ggggcctcgg attccgtgcg caaccggccg atccgtctcg tttcatctgg ggtgtcgtgc
    16981 cctcgctggt cgcctgggtc gcggtggtga tgccccccta tgccggactg gtcgtgcatg
    17041 gcgtgatgct gatcgtctgt tacggggtcg accgccgcat ttatccggtg caggctgcag
    17101 cggcctggct gacgctgcga tttcgcctga ccctcgtcgc cagcctgagc tgcttcatcg
    17161 gcgcagccgg cagctgaagc gggcgtgacc acgacgtccg aacatgcctg aaggtccgga
    17221 attccgctgc gttgaccaga tggtgcatag cggaatggct cgcgctgcag ggtggtcgca
    17281 gcaggcgctt gcgcaccccc gccggctctc cgaccgcatt gcaccgcttc cttgcccgta
    17341 acgcccatga tttcctaccg catcgacgtc cacaacgcac aagcgcacct gtttcgcgtc
    17401 acgctgacca tccctgcacc cggctcgacg cagcggctca gcctgcccgt gtggataccc
    17461 ggcagttacc tggttcgcga gttcgcgcgc cacctgagcg ccctcgaggc aaggcagggc
    17521 agccggccgg tggcgctgac gcagctcgac aagacgacct ggcaggccga gtgcagcgga
    17581 cgcgctgcgc tggtcgtcac gtacctggtc tatgccttcg acacctcggt gcgtgcggcg
    17641 ttcctggatg ggcaccgcgg cttcttcaac ggcaccagcc tgtgcctgcg cgtcgagggg
    17701 cgcgagtccg ggccgcaccg catcgagctg aagtcgctgc cgcgcggttg gcagatcgcg
    17761 acgtcgatgg cctcgcgctc cgctgccgtg ttcgaggccg tcgactacga cgatctggtc
    17821 gatcatccgt tcgagctcgg taccttctgg cgaggccgct tcgacgcgca cggtgtgccg
    17881 cacgaactgg tcgtggccgg cgcactgccc gacttcgacg gcgagcgcct gctggccgac
    17941 acgaagcgca tctgcgaagc cgagatcgcg ttctggcatg gtgaagccgc gaagcgcgcc
    18001 gacatcccgt tcgggcgata cgtgttactg ctgaacagcg tcgacgacgg ttacggcggc
    18061 ctggagcatc gcgcgagcac cgccctgatc gccgcgcgcc gtgacctgcc gcgcctggcc
    18121 gctcctggcg cgtccacagg cgccaagccg gaggagtcga ccgacggtta cgtgacgctg
    18181 ctcggcctga tcagccacga gtatttccac acgtggaacg tcaagcgact gaggccccgt
    18241 gacttcgcac cgtacgacta cacccgggag aactacaccg agctgttgtg gttcttcgag
    18301 gggttcacgt cctactacga cgatctgttc ctgcggcgtg ccgggctcat cgacgggccg
    18361 cgctacctga aggcggtcgg caagaccatc ggcagtgtgc tcggcatgcc ggggcgccag
    18421 gtgcagagcg tcgcgcaggc gagcttcgac gcctgggtca agtactaccg cggagacgag
    18481 aacacaccca acgcgacgat cagctactac acgaagggtt cgctggtcgc actggcgctg
    18541 gacctcagcc tgcggtcgac gaaatcgcgg cgcaaggcca cgctcgatga cgtgatgcgt
    18601 gccttgtggg tgcgcagcgc aggcgggccg atcggcgaag ccgacatcgt cgcagtgctg
    18661 cgcgaggtgg ccggaccggc acgcgccgag gcgctgacgc ggcaactggg cgagtgggtg
    18721 cacggcatca gcgacttgcc gctgcctgag ctgctgaagc gattcggcgt gcaatggcag
    18781 tcgcagccag caacgctggc tcagcggctc ggtgtgcggg tcagcgaaag cgcgctcggc
    18841 ggagtcaagg tcactcacgt gctgcgtgga ggcgccgcgg agcgagccgg tgtgtcggcc
    18901 ggggacgagg tgctggcgct cggagactgg cgcttgcgtc ggctcgacga cgcgctcagg
    18961 ctgctgatgc ccggcaagcc ggcatcactg ctggtgtcgc gcgaccagcg actgttgact
    19021 ctgacggtcg acgtgccgtt gtcggcggac gccgtcggcg ctgtctcgct ggggatcgac
    19081 gcacgggccg cgcacgacgc gatcgcgctg cgtaccgcat ggatcctcgg ctgacaccgc
    19141 acacgccaac gaagttcgcg accggaggag gtcgcatggt cactgccggc cgtgtcgcgc
    19201 tggtggccag cgtcgtcgcg atgcacgcct gcgtgatgca cggcatcaac cagcagatgc
    19261 tcgatttcga gactgcctcg cagatgcctg tgcgcatcga actggtctac gggcgcgaca
    19321 tggagtcgac cgtgccaccg gcgccggtcg ccgcgcccgc agtgccagtc gccgcgccgc
    19381 ctccgcgccc cgagcggcag gccaccccgg tggccgacgc cgcgtctgca ccagccgagc
    19441 cactcgaggc cccgatggcg gtggctgatg ctccgctgcc ggcagcgtcc gacgcggccc
    19501 cgatggtccc cgacgcgacg gcttcaatcg ctgcctccgc accggggctg gcctcggtca
    19561 tcgggccgac atctccggcg gcctcttcgc ctgccgattc cgcgagcggt ctggcggccg
    19621 ctgcgcccgc ggcttcggcc gccgccgaga cctactcctg gccgccttcc acccggatca
    19681 cctacgagct gaccggcaac taccgtggcg aggtctacgg cagtgccacg gtcgactggg
    19741 tgcgggaggg cgcgcgctat caggttcatc tggacgtgct cgtgggcccg gcgttcgcgc
    19801 cgatcctgtc ccgccgcatg accagcgaag gacagttgac cgacgcgggg ctctatccgg
    19861 agcgctacga cgaggacagc aaggccatgt tccgcgatcg aaggcgcgcc caggtcatgc
    19921 tcgagcccga tgcggtggtt ctcaacagcg gccagcgcgt cgagcgcctg gccggcgtgc
    19981 aggacacggc cagtcagttc gtgcagctcg cgtatctgtt ctcccgccac cccgaaatgc
    20041 tgaagcccgg cggaacggtg gagatgccgc tggcgctgcc ccgccggatg agcgtctgga
    20101 cctacgacgt gcgcgaagcc gaggtgctgt acaccccctt cggcgcgatc gatgccgtgc
    20161 cgcttcagcc gcgccgcgaa tcgcgcaaga aggacgagtt gtcggcgcag gtctggttcg
    20221 cgccgagcct gcggtatctg ccggtgcgca tccggatcca gcaggacacc aacaccgaca
    20281 cctacgtcga cctgatgatc gagcgtcggc cacagattgc cgggccatga tcaccggcca
    20341 gattcgaatt acctgttcag gagatgccca tgacccatga attcatcctc gtcgacacct
    20401 gcggcgacgg tcccctgaaa accggccgca tcaccctgaa ccggccgaaa cagctcaacg
    20461 cgctgaacga cggcctgatg agcgagctgg gggccgcgct gcaggctttc gatgccgatg
    20521 ccgggatcgg ctgcatcgtc atcaccggca gcgaaaaggc gtttgctgcc ggtgccgaca
    20581 tcggtgcgat ggccacactg aactacatgg acgcctacaa gggcgacttc atcacgcgca
    20641 actgggaggc gatccgcagc atccgcaagc cggtggtggc ggcggtgtcc ggctttgcgc
    20701 tgggcggcgg ctgcgagctg gcgatgatgt gcgatttcat catcgcggcc gacactgcga
    20761 ggttcggcca gcccgagatc aagctcggca tcatccccgg cgccggcggc acgcagcgcc
    20821 tgccacgcgc gatcggcaag gccaaggcga tggatctggt gctgaccggc cgcatgatgg
    20881 atgcgtccga ggccgagcgt tgcggactgg tgtcgcgcgt ggtgcccgct gccgatctga
    20941 tgacggtggc gctggaggcc gcgcagaaca tctgcgacta cagcggcccg agcgtgatga
    21001 tggccaagga gtgtgtcaac cgcgccttcg agggcacgct ggccgatggc atctccttcg
    21061 agcgcaggct gttccactcg ctgttcgcga ccgaagacca gaaggaaggc atggacgcct
    21121 tcgtcaacaa gcgcaagccc gacttcaagc accgctgacg cgggctgcct ggcacttcag
    21181 ggctgaaccc agcgcgcatg gccgtcgccg aacacggcac gccggtggcc gtcggcattg
    21241 agttccatcc agtcgatgca ggtgatggtg gccgtgatca gcgcgaaatg ctcgcgctgc
    21301 gctgccgctg gcgtgtgtcc cgccaggggc gtgcccggtg ccgtgggcga aaggtagtct
    21361 tgggcgcccg gcgacagccg gatatgggtc cagcgcgacg acacggccag cccgtcgtcc
    21421 tcgacgctgc agaccacgct gcatcgcaac tgccagccga gctcggccga ccacatgacc
    21481 agtgtcgcga ccggattgct gcgcaactgc gagaccttgc gcgcgcgcga atcggtgtag
    21541 atccgcaact gcctggccac cgtgtcggct tcgcgaagta cgaccagccg ggcgtcggca
    21601 accggctggc cggcttgatc cggcgccatc gaagcgagca cgcaggtgcg ccaggcatgg
    21661 ccgcgggcgc gcacgcagcg ctgcaactcg tcccagacag cggcttcaac ttcctccaga
    21721 gtcgacagcc gctggttcat cgtcaggctt ccttgcgcaa tgacgggaaa aggatcacgt
    21781 cgcggatgct cgggctgtcg gtgatcagca tcatcaggcg gtcgatgccg atgccgcagc
    21841 ctccggtcgg tggcatgccg tattccagcg cgcggatgaa gtcggcgtcg tagtacatcg
    21901 cctcctcgtc gccggcttcc ttgttggctg cctgagcgtg gaaacgtgcc gcctggtctt
    21961 cggcgtcgtt cagttcgctg aatccgttgg cgtattcgcg gccggtgatg aacagctcga
    22021 agcgttcggt gatcgccggg ttggtgtcgg acgctcgggc cagcggagac acctcgaccg
    22081 gatagtcgat gatgaaggtg ggattccaga gcttctcctc gacaaccgcc tcgaacagtc
    22141 cgaactgcaa ttcgggcagc ttccagtggg caggtggttc ctcgcccagc gacttgagct
    22201 tgtcgtgtaa cacctttgcg tcgccggctt cctcgaaggt caggcccgca tgcgccacca
    22261 gcgattcgcg caccgtcatc cgcgtgaaag gtgcatccag atcgaccgtc ctgcccgcgt
    22321 aggtcagcgt cgccgagccg gttgcgccgc gcgccgcgtg gcgcagcacc tgctcggtgt
    22381 agtccatcag gtcgtggtga ttccagtagg ccgcatagaa ctccatcatc gtgaattccg
    22441 ggttgtgccg gacgctgatg ccttcgttgc ggaagttgcg gttgatctcg a
//
LOCUS       DAOORV010000053        21930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2908_length_21930_cov_9.132069, whole genome shotgun sequence.
ACCESSION   DAOORV010000053 DAOORV010000000
VERSION     DAOORV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_21930_cov_9.132069"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2757
                     /gene="mfd"
                     /locus_tag="PKV56_08945"
     CDS             <1..2757
                     /gene="mfd"
                     /locus_tag="PKV56_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOX67981.1"
                     /translation="WREKMEGDPTKVRLYKDIGTGIATAGIEYYLPLFFDSTATIFDY
                     LGESASLVLHGEIDEALKRFWTDTRERHRFLQHDRERPILPPEDLFLKPEDFFTLGSA
                     LAVLAVRGNDPVDWARPLPDLTADRGAPEPLARLQQHVGSTAHRVLIVAETEGRRESL
                     LELLRDSRIEPPSVATLAEFEAGEERFAITAAPLAVGFFWHEPQAGKAIQFVTETELF
                     ATSPTARRRRKQEQVSDVNALIKDLSELKVGDPVVHINHGIGRYVGLRNIDLGDGDSE
                     FLHLEYADKATLYVPVAQLHLISRYTGVSAEEAPLHRLGSGQWEKAKRKAAEQVRDTA
                     AELLNLYARRAAREGYAFRFSAHDYEAFAASFGFEETPDQKAAIHAVIQDMVSPKPMD
                     RLVCGDVGFGKTEVALRAAFVAVTGGKQVAILAPTTLLAEQHFQNISDRFGKWPIKVA
                     EMSRFRSAKEIKQALEGLEDGTIDIVVGTHKLLSQNTKFKRLGLLVIDEEHRFGVRHK
                     EAMKALRAEVDVLTLTATPIPRTLGMALEGLRDLSVIATAPQRRLAIKTFVRSESSGT
                     IREAVLRELKRGGQVYFLHNEVETIENRRSKLEELVPEARIAIAHGQMPERELEKVMR
                     DFVAQRHNVLLCSTIIETGIDVPSANTIVISRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLVPDVEGLTKQAQQRLQAIQDMEELGSGFYLAMHDLEIRGAGEVLGENQSGNMMEVG
                     FQLYNEMLAEAVRSLKAGKEPDLLSPLSVTTEINLHAPALLTTDYCGDVHTRLSLYKR
                     LASATKNEQIDAMLEEITDRFGKLPPQGQTLFDVHRLRVLAKPFGVIKIDAAPTLMVI
                     SFRPNPPIDAMKIIDLVQKNRHIKLAGNDKLRIERATPEAKDRAQLIREVLRALGKPT
                     SVPG"
     gene            2892..3728
                     /locus_tag="PKV56_08950"
     CDS             2892..3728
                     /locus_tag="PKV56_08950"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX67982.1"
                     /translation="MNPESRFLTHPLRDVNAWVNYFRNAEIPVLSYTAQRIDEMAADE
                     DSADVNVLADTINDDPLMTLRLLRHVSNLRKGNRGSAPETVLEALVLIGVCPLFRAFE
                     HMPSVQDWLADWPEALDGLSHVIAQGYRAARFAQGFAAHRSDPDASVIHEVALLHHVV
                     EMLFWCHAPALALEIVRRQAAQPGLRTIQAQKEVLGIDFRELRLSLMKEWKLPELLVQ
                     LVDDGRSSNSQVQSVLLAVRLARHTTHGWDHPALNDDIRDVSELLHLGIEPTRRLLSQ
                     LA"
     gene            3853..4566
                     /gene="serB"
                     /locus_tag="PKV56_08955"
     CDS             3853..4566
                     /gene="serB"
                     /locus_tag="PKV56_08955"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOX67983.1"
                     /translation="MNHPTVSTGLTCRGFVPPLRLGDFKLIAFDMDSTLINIECVDEI
                     ADAAGRKAEVAAITEAAMRGEITDYKDSLRRRVALLRGVPVAAMAEVYEQRLQLNPGA
                     ELLVRACQDVGLVTLLVSGGFTFFTDRVRDRLGIQYTRSNVLEVENDHLTGRMVDQPW
                     GDICDGEEKRRMLLWTCFHVGCEPREAIAVGDGANDLPMMGEAGLSVAYHAKPRVREQ
                     AMVAIDAGGLDRLLEVVRP"
     gene            complement(4671..5180)
                     /gene="rraA"
                     /locus_tag="PKV56_08960"
     CDS             complement(4671..5180)
                     /gene="rraA"
                     /locus_tag="PKV56_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HOX67984.1"
                     /translation="MTATPTSSSSSTCDLCDVHKNDSSGSFRVLPPVFRDYGKRIRFA
                     GPISTVKCFEDNTAVKAALEEPGLGRVLVVDGGGSMRRALVGGNIAASAARNGWAGVV
                     VDGCVRDSAELAESDVGIRALALVPLPTDRKNQGLRDGVLLIQGVTVRPGEWLVADED
                     GIVVLATPP"
     gene            complement(5256..6560)
                     /locus_tag="PKV56_08965"
     CDS             complement(5256..6560)
                     /locus_tag="PKV56_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX67985.1"
                     /translation="MNPPAAPAVADAGTSVLLRDDARTIALVGLAHGTSHFFHLLLPP
                     LFPLFIRDFGFSYAQLGLLVTTFFVVSGIGQALAGFVVDRTGARPVLFAALSFFALSG
                     VAVSLSTGYGGLMFAAALAGLGNCPFHPADFTILNRRVSTSRLGHAFSVHGLTGNLGW
                     AAAPVFMTGLAAAFGSWRWAAACASLLALAVIAILWWQRDAIDDHQGLAASPAVRRAS
                     DRGPTAMDGEHPLAFLRLPSVWLCFSFFFWSTSALSGIQSFSSPALQQLVGLPLSVTS
                     LVVTGYMLFGAVGIVIGGFLAAHSDRLERIIAACLLVSAALLALVGSGVVPGTMAALL
                     TAAAGFGAGLAGPSRDMLIRQAAPPGATGRVYGAVYSGLDLGFAVAAPVFGWLLDRGF
                     PSGVFYGSALALSVGVASAAAVGFGIERRRGTPVAHRAIRAG"
     gene            6704..7558
                     /locus_tag="PKV56_08970"
     CDS             6704..7558
                     /locus_tag="PKV56_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX67986.1"
                     /translation="MKQIAAPRRPEPGRRRRTSVPPIDPQVYAPSGSHPLRARMRHLA
                     ADTRVEPHSHPWAQVALSNRGVIRMTAGQSTYLVPPQRALWIPPGVEHVVTVVEDADL
                     HTLYIHQDDGQAGPLRSSDEAGVWRQCRVLEVSRLLRELVQHLPLEPGSATPTERERC
                     IAQLVLDELAHARPVRLGVDLPRDKRLRALCETVLDDPARWTTLEETARHAGASARTF
                     ARLFRSELGTSFVQWRQQVLLARALSMAARKLPMSAIAAELGYASASAFSAMVRRSVG
                     MPPSRFLG"
     gene            7648..8100
                     /locus_tag="PKV56_08975"
     CDS             7648..8100
                     /locus_tag="PKV56_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67987.1"
                     /translation="MHPLTPFEQQVLEAALQGPQPVLAALRSQIGHAKVSARTHTGVG
                     SYIDFSVPQSVPRVSPTEMVFGDVDVQVEGVSAEVSTILYIVGGHLHFLEFAALSGEW
                     PKEPVITKLGYLRSEKVGPDDYKAVAVDERDADTLERSLAGPAAKKKI"
     gene            8142..9248
                     /gene="nadA"
                     /locus_tag="PKV56_08980"
     CDS             8142..9248
                     /gene="nadA"
                     /locus_tag="PKV56_08980"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOX67988.1"
                     /translation="MTQVLFDYTRQDATGASCTARAWAKVPPSLTASERTAWKARAAA
                     LLKQHDAVLVAHYYVDGDLQDLALETGGCVADSLEMARFGRDHASRTLVVAGVRFMGE
                     SAKILSPDKRVLMPDLDATCSLDLGCPPDAFAQFCDAHPDRTVVVYANTSAAVKARAD
                     WMVTSSCALAIVQHLKDQGQKVLWAPDRHLGRYIQQQTGADMLMWNGECIVHDEFKGL
                     ELALLKKQYPEARVLVHPESPETVVALADVVGSTTQLLNAVTTMDAQTFIVATDNGLM
                     HRMRQLAPTKTLLEAPTAGDSATCKSCAHCPWMAMNALQGVVDCLESGHGEIQVAEPV
                     RSQALGCIDRMLDFVKAHPTATQKPGFVAHIGAA"
     gene            9257..10123
                     /gene="nadC"
                     /locus_tag="PKV56_08985"
     CDS             9257..10123
                     /gene="nadC"
                     /locus_tag="PKV56_08985"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOX67989.1"
                     /translation="MFEINEDLEAARARNVRDALMEDIGQRDWTAELVPAGQRVSARV
                     LVREEAVLCGRDWFDACIAAMDPAARVDWHVAEGALMGADTRVCDITSDARALLSAER
                     PALNFLQLLSATATLTRRHVDAIAGASPNPRGCVILDTRKTLPGLRQAQKYAVRTGGG
                     RNQRLALWHGILIKENHIAAAGGITAVLRNAAALNAGVDIQIEVETLEQLREALAAGA
                     VSVLLDNFDLDGMRAAVALSAGRALLEVSGGVRLDQVREIAATGVDRISIGRLTKDVR
                     ATDYSMRVVSTL"
     gene            10232..10519
                     /locus_tag="PKV56_08990"
     CDS             10232..10519
                     /locus_tag="PKV56_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOX67990.1"
                     /translation="MSTVFDSIHNHYEHAVFDAVLAASSRFSHLSDPHIMADVACVAL
                     NRLPPRYIRHDVDFSFYLTERERSENETAIAEAVEFAFQFVQAKVAMRARR"
     gene            complement(10532..12172)
                     /gene="nadB"
                     /locus_tag="PKV56_08995"
     CDS             complement(10532..12172)
                     /gene="nadB"
                     /locus_tag="PKV56_08995"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOX67991.1"
                     /translation="MPLIDPARAQLPVVIIGAGLAGLTVALHLAVDHPVIVLAKRGLD
                     EGATAWAQGGIVGVLGDDDSIDSHVRDTQDAGAGLVDEHTARFIAQHSAEAIGWLVDR
                     GVPFSADPEGPLGLHLTREGGHAVRRIAHAADATGKAIHDVLLDEVRRHPNIDMRERW
                     MAVDVITSRQLRRDEQPRCYGVYALDIDQQRVETLPARAVVLATGGVGKVYRYTSNPD
                     TATGDGIAMAWRAGCRIGNMEFIQFHPTCLYHPQERSFLITEALRGEGGHLKLPDGTR
                     FMAAHDERMELAPRDIVARAIDFEMKKHGLDHVWLDATHLGEAFLKEHFPTIHARCLA
                     LGIDIARQPIPVVPAAHYTCGGVVTDLHGRTDLPGLYAVGETTYTGLHGANRLASNSL
                     LECVVLGRTCAMDIHELGDHPEPHLPAWDESQVENADEQVVIAHNWDELRLLMWNYVG
                     IVRTTKRLERALHRIKLLRSEIDDYYGSFRVNRDLLELRNLVDCAELIVRSALMRHES
                     RGLHYSRDFPQALPVSFPTVLMRRTRVGRKPKTHRLSV"
     gene            12397..13755
                     /locus_tag="PKV56_09000"
     CDS             12397..13755
                     /locus_tag="PKV56_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67992.1"
                     /translation="MSNYTSRTGSPYTDRPAPPPIENDGWESTRAQTALATQIMDREG
                     DAPPTYRQEIGRPVGNDVRELFVACAPGEALQQQFEHLHPEFIAVHDLATRSSRQLLR
                     GIAAASNRAVQKLVLRRQGGGAPVATIDFIEVPVSAQETLRIYSTDIDDTTDAAARHA
                     VARTLLAYSQLGVMMVGAMPAENIHALLRPWHEELIRSPWLNRNLLLLPLTNTPALTT
                     NGQNMVRGTSITLRTTPVVTRPADAWNFINGTWGRLRGAVSPLGAPAAPARSVTTARA
                     ATSQPPVQSQAAARVSAQIDAGYRPPAAPDDPSGFAPTNLLPLRPMPDVRARPVPLQD
                     EVLEVYVHQVCELNGVVGCCVFDIGSGLPVAHGGAGAPPEELARHGRALLSAMGTTSR
                     ALGLGHTLPDAAITLGSHHLLLRSVAKHAGMALLTVLDRTNANLTLARLQIERLDPLL
                     DR"
     gene            complement(13890..16481)
                     /gene="clpB"
                     /locus_tag="PKV56_09005"
     CDS             complement(13890..16481)
                     /gene="clpB"
                     /locus_tag="PKV56_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOX67993.1"
                     /translation="MRLDKLTTTFQEALADAQSLANGRDNPYMEPVHVLSAMLAQADG
                     PKALLARAGVNTAKLQGALEAELKKLPQVQGGEQVQPSRDLGALLQAAEKESTKRGDQ
                     FIASEMFLLAVADSKTAIGPLVKEHGLNRKALEAAIDAVRGGQKMDSAEGEGQREALK
                     KYTLDLTERARQGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLA
                     QRIVAGEVPDSLKNKRVLVLDMALLLAGAKFRGEFEERLKSVLKEVAQDEGQTIVFID
                     EIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLNEYRKYVEKDAALERRFQKI
                     LVGEPSVEATIAILRGLQEKYEVHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAAAKVKIEIDSKPEAMDKLDRRLIQLQIEREAVRREKDEASQKRFGLIEEEITKLQ
                     REAADLDEIWKSEKAAAQGSAHVKEEIDRIRFQIEDLKRKGDFNKVAELQYGRLPELE
                     KKLAEAQSKEQGKAKAGGKPQLLRTMVGAEEIAEVVARATGIPVSKLMQGEREKLLQM
                     ETRLHERVIGQDEAITAVANAIRRSRSGLSDPNRPTGSFMFLGPTGVGKTELCKALAG
                     FLFDSEEHLIRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGYLTEAVRRNPYSVLLLDE
                     VEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNCVIVMTSNLGSHQIMQMAGQDSELI
                     REAVWVEVKQHFRPEFLNRIDEVVVFHALDHRHIESIAGIQLKVLESRLEKMDMKLEV
                     TPQALAELAKAGFDPVFGARPLKRAIQQRIENPVAKLILEGKFGPKDVIPVGVADGEF
                     VFDRVVH"
     gene            16637..16924
                     /locus_tag="PKV56_09010"
     CDS             16637..16924
                     /locus_tag="PKV56_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67994.1"
                     /translation="MNPTPTTAALAGIFSALAWPLLWPWLSESTASSSVWLVVGTLLL
                     IALPAHAFVVGFKRNPLPGARAIDTALLKRIGAWLLAAALTSFIMSLLPPT"
     gene            complement(16980..18029)
                     /locus_tag="PKV56_09015"
     CDS             complement(16980..18029)
                     /locus_tag="PKV56_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX67995.1"
                     /translation="MSYCAGWKHKGSVYLLADTVSSVQIPPRRQQISLGELLTEHHDE
                     PIEEPALKLVRIAPGAVAACTGDAGSVSRLLEFLQANHAGVTNNGELLSLLDVSLGPF
                     SDAKPVSLLLASSGSDGACELLRWTSPGGLDTTGSDLLQIGDPTPFHTALSPEILSIL
                     AAADPSPERMLPVLTAIVQSRGVSENSSDLNVNGLIFGVRTGLGVATWQEDTLVVLYD
                     QAFASPAHVAVLARSDELVVHASHDAATRVFVPLPSNHRRAPREACWLRGIQDELAAD
                     RFRFRVFISTCEQVITLIIRNDFERESGYVRLKRKSNGQFDFALSPEMTSLLLRPLED
                     RSQGSVPLRLSLRED"
     gene            complement(18054..18443)
                     /gene="crcB"
                     /locus_tag="PKV56_09020"
     CDS             complement(18054..18443)
                     /gene="crcB"
                     /locus_tag="PKV56_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOX67996.1"
                     /translation="MNQSVPWAQLMAVALGSAVGGVLRWAVQLWLNARWAGFPLGTLV
                     VNCVGGLLIGIALAWFARTPNESMRLLLVTGFLGGLTTFSAFTGESLTLLQRGDFGWA
                     VAHSFAHLAGALLCCAMGFRVAKTLLA"
     gene            complement(18440..18925)
                     /gene="moaE"
                     /locus_tag="PKV56_09025"
     CDS             complement(18440..18925)
                     /gene="moaE"
                     /locus_tag="PKV56_09025"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HOX67997.1"
                     /translation="MSVSIQTADFDLSAEVAALRSADPGVGAVATFVGCVRDRNDGSG
                     VSAMELEHYPGMTEKSIESMISAARRRFDIRAVRVIHRVGLLQPQDQIVLVAVASSHR
                     GSAFRACEFLMDYLKTQAPFWKKESTPEGSRWVDARVSDDEAMARWQQGEPDPATEPD
                     R"
     gene            complement(18951..19202)
                     /locus_tag="PKV56_09030"
     CDS             complement(18951..19202)
                     /locus_tag="PKV56_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOX67998.1"
                     /translation="MNVQVRYFASIREVLGAGESIEVPAGATIGELRDRLIASSAAHA
                     TALSRERSLRSALNQLLCDESEPVGADAEVAFFPPVTGG"
     gene            complement(19199..20509)
                     /locus_tag="PKV56_09035"
     CDS             complement(19199..20509)
                     /locus_tag="PKV56_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOX67999.1"
                     /translation="MSTNHPTSTPPIGDAAAPAARAPLLGLDEALDRLTQAVQPFGVV
                     RAQQVSTFDALGRVLAEEVRSVVDVPPADNTSMDGYAMRCADVSAVGIALPVSQRIPA
                     GVAGTPLVPGTAARIFTGAQVPAGADAVVMQEQCVALDGGAVRIDAMPSPGQWIRRRG
                     EDVLAGSVVLPKGTRLTPQALGLAASVGAASLSVARRPRVALFSTGDELVMPGEPLKP
                     GAIYNSNRFTLRALVQALGCDCTDFGIVPDRLDATREALRRAAQEHDLIVTSGGVSTG
                     EEDHIRPAVEAEGRVTLWQMAIKPGKPLAFGEVRREPLRSGESADSGAAYFIGLPGNP
                     VSSFITFLLVVRPVLLHLQGAVDWQLRSLPMRADFDWPKPDRRREFLRVRRNSSGGLD
                     LFANQSSGVLTSAVWGDGLVDNPSGQAIRSGDMVQYLGFAELLS"
     gene            complement(20469..21008)
                     /gene="mobB"
                     /locus_tag="PKV56_09040"
     CDS             complement(20469..21008)
                     /gene="mobB"
                     /locus_tag="PKV56_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOX68000.1"
                     /translation="MKVIGFCGYSGSGKTTLVEQLIALLREKGLRVSVVKHAHHAFDI
                     DHPGKDSWRHRRAGASEVVVASNRRLAVIREFAAIAEPTVHELIAELSSCDWVLVEGF
                     KHADLHKVEVWRAETGNPVQYPHDPFIVAVCTDSATLPVPTDLPVLDLNDVGAVANFL
                     LSDSARHEYQPPHLDSPDR"
     gene            complement(21038..>21930)
                     /locus_tag="PKV56_09045"
     CDS             complement(21038..>21930)
                     /locus_tag="PKV56_09045"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801616.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOX68001.1"
                     /translation="FQQAQMFSLQDANIHNIAIDGVFDDCQDIVKAVSNDLDFKRRHH
                     IGTVNSINWARLLAQVVYYFAGYFQATRSNDQQVSFAVPSGNFGNICAGHVARMMGLP
                     IRYLVLATNENNVLDEFFRTGTYRVRGSAETHETSSPSMDISKASNFERFVFDLLGRD
                     GARTKQLFKTEVEQKGSFTVSDDEFANTASFGFVSGTSTHADRLATIRDTYRRFGVII
                     DTHTADGLKVGREHLDWGVPMLVLETALPAKFAETIVEAIGVQPDRPAAMRGIEDLPK
                     RFVVMPVDTQAVKAYLSANV"
BASE COUNT         3848 a         7412 c         7160 g         3510 t
ORIGIN      
        1 tggcgcgaga agatggaagg cgacccgacc aaggtgcgcc tgtacaagga catcggcacc
       61 ggcatcgcca ccgccggcat cgagtactac ctgccgctgt tcttcgacag cacggcgacg
      121 atcttcgact acctcggcga atcggcctcg ctggtgctgc acggcgagat cgacgaggca
      181 ctgaagcgct tctggaccga cacgcgcgag cgccaccgct tcctgcagca cgatcgcgag
      241 cggccgatcc tgccgcccga agacctgttc ctcaagccgg aggacttctt caccctgggc
      301 agcgcactgg ccgtgctggc ggtgcgcggc aacgaccccg tggactgggc gagacccctg
      361 cccgacctca ccgccgaccg cggcgcgccc gagccgctgg cccggctgca gcagcatgtg
      421 ggcagcacgg cgcatcgcgt gctcatcgtc gccgagaccg agggccgccg cgagagcctg
      481 ctcgaactgc tgcgcgacag ccgcatcgag ccgccctcgg tggccacgct ggccgagttc
      541 gaagccggcg aagaacgatt cgccatcacc gccgcgccgc tggcggtggg cttcttctgg
      601 cacgagccgc aggctggcaa ggcgatccag ttcgtcaccg agaccgagct gttcgccacc
      661 tcgcccaccg cgcggcggcg gcgcaagcag gagcaggtca gcgacgtcaa tgcgctgatc
      721 aaggacctgt cggagctgaa ggtcggcgac ccggtcgtgc acatcaacca tggcatcggg
      781 cgttacgtcg gcctgcgcaa catcgatctc ggcgatggcg acagcgaatt cctgcacctc
      841 gagtacgccg acaaggcaac gctctacgtg ccggtcgcgc agctgcacct gatcagccgc
      901 tacaccggcg tcagtgccga agaggcaccg ttgcaccggc tgggttcggg ccagtgggaa
      961 aaggccaagc gcaaggccgc cgaacaggtg cgcgacaccg ccgccgaact gctgaacctc
     1021 tatgcccgtc gcgccgcgcg cgagggttat gcgttccgct tctcggcgca cgactacgaa
     1081 gccttcgcgg ccagcttcgg cttcgaggaa acgcccgacc agaaggcggc gatccacgcg
     1141 gtgatccagg acatggtgag cccgaagccg atggaccggc tggtctgcgg cgacgtcggt
     1201 tttggcaaga ccgaggtggc cttgcgcgcc gccttcgttg cggtgaccgg cggcaagcag
     1261 gtggccatcc tggcaccgac cacgctgctg gccgagcagc atttccagaa catctccgac
     1321 cggttcggca agtggccgat caaggtcgcc gagatgtcgc gattccgctc tgcgaaggag
     1381 atcaagcagg cgctcgaagg gctcgaggac ggcacgatcg acatcgtcgt cggcacgcac
     1441 aagctgctga gccagaacac gaagttcaag cggctcggcc tgctggtgat cgacgaggaa
     1501 caccgcttcg gcgtgcgcca caaggaggcg atgaaggcgc tgcgcgccga ggtcgacgtg
     1561 ctgacgctga ccgcgacgcc gatcccgcgc acgctgggca tggcgctgga aggcctgcgc
     1621 gacctgagcg tcatcgcgac cgcaccgcag cggcggctcg cgatcaagac cttcgtgcgc
     1681 agcgagtcga gcggcacgat ccgcgaggcg gtgctgcgcg agttgaagcg cggcggacag
     1741 gtttatttcc tgcacaacga ggtcgagacg atcgagaacc ggcgcagcaa gctggaagaa
     1801 ctcgtccctg aggcgcgcat cgcgatcgcg cacggccaga tgcccgagcg tgaactcgaa
     1861 aaggtgatgc gcgacttcgt cgcgcagcgc cacaacgtgc tgctgtgctc gaccatcatc
     1921 gagaccggca tcgacgtgcc cagcgccaac accatcgtga tcagccgcgc ggacaagttc
     1981 ggcctggcgc agctgcacca gttgcgcggc cgcgtcggac gctcgcacca tcaggcctat
     2041 gcctacctgc tggtgcccga tgtcgaggga ctgaccaagc aggcacagca gcggctgcag
     2101 gcgatccagg acatggagga actcggctcc ggcttctacc tcgcgatgca cgacctcgag
     2161 atccgcggcg ccggcgaagt gctcggcgag aaccagagcg gcaacatgat ggaggtgggc
     2221 ttccagctct acaacgagat gctggccgag gcggtgcgat cgctgaaggc cggcaaggaa
     2281 ccggatctgc tgtcaccgct ttcggtgacg accgagatca acctgcacgc gccggcgctg
     2341 ctgacgaccg actactgcgg tgatgtgcac acccgcctga gcctgtacaa gcggctcgca
     2401 tcggcgacca agaacgaaca gatcgacgcg atgctcgagg agatcaccga tcgattcggc
     2461 aagctgccac cgcaaggcca gacgctgttc gacgtccacc gcctgcgcgt gctggccaag
     2521 ccgttcggtg tcatcaagat cgacgcggcg ccaacgctga tggtgatcag cttccgcccc
     2581 aatccaccga tcgacgcgat gaagatcatc gatctggtgc agaagaaccg ccacatcaag
     2641 ctggccggca acgacaagct gcgcatcgag cgcgccacgc ccgaagccaa ggatcgcgcc
     2701 cagttgatcc gcgaggtgct gagggcactc ggcaaaccga cttcggttcc aggctgactc
     2761 cgcaggagtc gcgaaaacgg gtgcacgacc acgggaaagc ccgaggtcac gacagggttc
     2821 aatctcgaac gcaagcggcc gataacccgg ctggggtcgg cctgtttacc gaccttcggg
     2881 accggctgac catgaaccca gaatcacgat tcctgacgca tccgctgcgc gacgtgaacg
     2941 catgggtgaa ctacttccgc aacgcggaaa tcccggtcct gagctacacc gctcagcgca
     3001 tcgacgagat ggccgccgac gaggattcgg cggacgtcaa tgtgctggca gacaccatca
     3061 atgacgatcc gctgatgacg ctgcgcctgc tgcgccacgt gtccaatctg cgcaagggca
     3121 accgcggatc ggcgccggag acggtgctcg aggccctggt gctgatcggc gtgtgcccgc
     3181 tgtttcgcgc cttcgagcac atgcccagcg ttcaggactg gctggcagat tggcccgagg
     3241 cactcgacgg cctcagtcat gtcatcgcgc agggctatcg cgcagcgcga tttgcgcagg
     3301 gcttcgcagc gcaccgcagc gatcccgatg cgtcggtgat ccacgaggtt gcgctgcttc
     3361 atcacgtggt cgagatgctg ttctggtgtc atgcgcctgc gctggcactg gagatcgtcc
     3421 gccgccaggc tgcgcagcct ggtctgcgca ccatccaggc acagaaggag gtgctcggca
     3481 tcgacttccg cgagctgaga ctgtcgctga tgaaggaatg gaagctgccc gagctgctgg
     3541 tccagctcgt cgacgatggc cgatcgagca actcgcaggt tcaaagcgtg ctgctggcgg
     3601 tcaggctggc gcgtcacacg acgcatggct gggatcatcc cgcactgaac gacgacatac
     3661 gcgacgtctc cgagctgctg catctcggca tcgagccgac acgccggctg ctttcacagc
     3721 tggcctgacg gggttcgcta accgagccgg atcgttcgtc ctggatcgag gacggcgcag
     3781 cgcgtgcggg ttcgggacaa tacgcggatg acccgatcag cggtcttcca gcgcttgtcg
     3841 cccctgacac ccatgaatca ccccactgtt tctaccggcc tgacgtgccg aggtttcgtg
     3901 cccccgctgc ggctcggcga cttcaagttg atcgcgttcg acatggactc cacgctgatc
     3961 aacatcgaat gcgtcgatga gattgccgac gcagccgggc gcaaggcgga ggtggcggcc
     4021 atcaccgagg ccgcgatgcg cggcgagatc accgactaca aggacagcct gcgccggcgg
     4081 gtcgcgctgc tgcgcggcgt gcccgtggct gcgatggccg aggtctacga gcagcggctg
     4141 cagctcaatc cgggcgcaga gttgctggtg cgcgcctgtc aggatgtcgg gctggtgacg
     4201 ctgctggtgt cgggaggatt caccttcttc accgaccggg tgcgcgacag gctgggcatc
     4261 cagtacaccc gcagcaacgt gctggaagtg gagaacgatc acctgaccgg acgcatggtg
     4321 gatcagccct ggggcgacat ctgcgacggc gaggaaaagc gtcgcatgct gttgtggacc
     4381 tgctttcacg tcggctgcga gccgcgcgag gccatcgcgg tgggcgatgg ggccaacgac
     4441 ctgccgatga tgggcgaagc cggcctgtcg gtggcatacc acgccaaacc tcgcgtgcgc
     4501 gagcaggcga tggtcgcgat cgatgccggt ggtctcgatc ggctgctgga agtcgttcga
     4561 ccgtaagtcg cgacggttgc ccggagcggg cctgtcgaag aacgggcgcg tcaccggacc
     4621 cgcccgtgcc tggcttggct cagggctcgc aggccgcggg ccgaggaaat tcagggcggg
     4681 gtcgccagga cgacgatgcc gtcctcgtcg gccaccagcc attcgcccgg gcgcacggtg
     4741 acgccctgga tcagcagcac gccgtcgcgc aggccctggt tctttcggtc ggtgggcaac
     4801 ggcaccagcg cgagcgcacg gatgcccacg tccgattcgg cgagttcggc gctgtcgcgc
     4861 acgcaaccgt cgacgacaac gccggcccag ccgttcctcg ctgccgaggc ggcgatgttg
     4921 ccgccgacga gggcgcgccg catcgagcca ccgccatcga ccaccagcac ccggcccagg
     4981 cccggctcct ccagcgcggc cttcaccgcg gtgttgtcct cgaaacactt gaccgtgctg
     5041 atcgggccgg cgaagcggat gcgtttgccg taatccctga agacaggtgg cagcacgcgg
     5101 aacgagccgg agctgtcgtt cttgtgcacg tcgcagaggt cgcaggtgct cgagctggag
     5161 gaggtgggtg tagctgtcat ggtggggatc ggttgaacga agcctccact gtgccatgcc
     5221 gacgcgaccg ctgagccgcc tgccgcgccg ctgagtcagc cggcccggat cgcccggtgg
     5281 gcaacaggcg tgccccgtcg gcgctcgata ccgaatccga cagccgccgc cgaggcgacc
     5341 cccacgctca atgcgagcgc ggatccgtag aacacccccg acggaaatcc tcgatcgagg
     5401 agccagccga acaccggcgc agccacggcg aagccgaggt ccagccccga gtacaccgcg
     5461 ccgtagaccc ggccggtcgc ccccggcgga gctgcctgcc tgatcagcat gtcgcgggac
     5521 gggccggcca ggccggcgcc gaatccggct gccgccgtca gcagtgcagc catcgttccc
     5581 ggaacgacac cgctaccgac cagcgccagc agcgccgcag acaccagcag gcaggcggcg
     5641 atgatcctct cgagccgatc gctgtgcgca gccaggaacc cgccgatgac gataccgacc
     5701 gcaccgaaga gcatgtagcc ggtcaccacc agcgaggtga cggacagagg cagtccgacg
     5761 agttgctgca gcgcggggct ggagaagctc tggatcccgc tgagcgcgct ggtcgaccag
     5821 aagaagaagg aaaagcacag ccacaccgac ggcagccgca ggaacgccag cgggtgctcg
     5881 ccatccatcg ccgtgggccc acggtctgat gcacggcgga ctgcaggcga cgcggcgaga
     5941 ccctggtggt cgtcgatcgc atcgcgctgc caccacagga tggcgatcac cgccagcgcc
     6001 agcagcgatg cgcacgcggc agcccacctc cacgagccga acgccgccgc cagtccggtc
     6061 atgaaaacgg gggcggccgc ccaccccagg ttaccggtca gcccatgaac gctgaaggca
     6121 tgtccgagcc gcgaagtcga gacacgacga ttcaggatcg tgaagtcggc gggatggaac
     6181 ggacagttcc ccaacccagc cagcgcagca gcgaacatca gcccgccgta tcctgttgag
     6241 agcgagaccg cgacaccgga caaagcgaag aacgacagcg cggcaaacag caccggacgt
     6301 gcgccggtgc gatcaacgac gaagcccgcc agggcctgcc cgatccccga gaccacgaag
     6361 aaggtggtga ccagaaggcc cagctgggcg tagctgaatc cgaagtcgcg gatgaacagc
     6421 ggaaacagcg gcggcagcag caggtgaaag aagtgcgagg tgccgtgcgc cagtccgacc
     6481 agcgcaatcg tgcgcgcgtc atcgcgcagc aggacggagg tacctgcatc tgcgacggcg
     6541 ggcgcggctg gcggattcat cgattggtgc gggacaaggc gtcgggagaa gacgggacgt
     6601 gccaatgtag tggcgtgcga tgcgtcgcaa tagcgatagt cggacaatga atatcgcaaa
     6661 tcagacaaat tcgcctgcgc cattcctcct gaaagcgccc agcatgaaac agatcgctgc
     6721 cccgcgtcgg ccggaaccgg gtcgacgccg ccgcaccagc gtgccgccca tcgacccgca
     6781 ggtctacgcg cccagcggat cccatccgct tcgcgccagg atgcggcatc tggctgctga
     6841 cacccgcgtc gaaccgcaca gccatccatg ggcgcaggtc gcgttgtcga atcggggcgt
     6901 gatccgcatg accgccggtc agagcaccta cctcgtgcct ccgcagcgcg cgctgtggat
     6961 tccgcctggc gtcgagcacg tggtgacggt ggtcgaggac gcggacctgc acaccctgta
     7021 catccatcag gacgatggcc aggccggacc gctgcgcagc agcgacgaag ccggggtttg
     7081 gcgccaatgc cgagtgctcg aggtttcgcg cctgctgcgt gagctggtgc agcacctgcc
     7141 gctcgagccg ggcagcgcca ccccgaccga acgggagcgc tgcatcgccc aactggtgct
     7201 cgacgagctg gcacatgcgc gcccagtgcg cctcggagtc gacctgcccc gtgacaagcg
     7261 cctgcgcgca ctgtgcgaga ccgtgctcga cgacccggcg cgctggacca cgctggagga
     7321 aaccgcccgg catgccggag ccagtgcccg aacctttgcg cgcctgttcc gatcagagct
     7381 gggaaccagc tttgtccagt ggcgtcagca ggtactgctg gcacgcgcgc tgtcaatggc
     7441 cgctcgcaag ctgccgatga gtgcgatcgc ggccgagctg ggctatgcca gcgccagtgc
     7501 gttcagcgcg atggtcaggc gatcggtcgg catgcctccg agccggtttc tgggctgata
     7561 ggccagcggt tccgagggca tgccgagcgc gaaccataat gtcgcccgcg gcgcgcggac
     7621 gcccgttctc attcagcaag gaatcccatg catcccctca ccccgttcga gcagcaggta
     7681 ctcgaagccg ccctgcaagg cccacagccg gttctggccg ccctgcgttc gcagatcggc
     7741 catgccaagg tgtcggcccg cacgcacacc ggcgtcggct cgtacatcga cttctccgta
     7801 ccgcagtcgg tgccccgggt cagtccgacc gagatggtgt tcggtgacgt cgatgtccag
     7861 gtcgaaggcg tgtcggccga agtctcgacg atcctgtaca tcgtcggcgg ccacctgcac
     7921 ttcctggagt tcgccgcgct ctcgggcgag tggccaaagg agccggtgat caccaagctc
     7981 ggctacctgc gatccgagaa ggtgggcccg gacgactaca aggcggtggc cgtggacgag
     8041 cgggatgccg acacactcga gcgttcgctg gccggtccgg cagccaagaa gaagatctga
     8101 cgaaccgtcg aatcgacggc ccagcccacc tcgaggcagc catgacccag gtcttgttcg
     8161 actacacgcg ccaggacgct acaggtgcga gttgcacggc tcgcgcctgg gccaaggttc
     8221 cgccgtcctt gacggcttcg gagcgcaccg catggaaggc ccgggctgcc gcgctgctga
     8281 agcagcacga tgcggtgctg gtggctcact actacgtcga tggcgacctg caggacctgg
     8341 ccctggagac cggcggctgc gtcgccgact cgctggagat ggcgcgtttc ggccgcgacc
     8401 atgccagccg cacgctcgtc gtggcgggag tgcgattcat gggcgagagc gccaagatcc
     8461 tgagcccgga caagcgggtg ctgatgcccg atctggatgc cacctgctcg ctggaccttg
     8521 gctgcccgcc cgatgcgttc gcgcagttct gcgacgcgca tcccgaccgc accgtcgtcg
     8581 tctatgccaa caccagcgcc gcggtgaagg cgcgcgccga ctggatggtg acaagttcgt
     8641 gcgcgttggc gatcgttcag cacctgaagg accagggtca gaaggtgctg tgggctcccg
     8701 accgccacct gggccgctac atccagcagc agaccggcgc cgacatgctg atgtggaacg
     8761 gcgaatgcat cgttcacgac gagttcaagg ggctcgagct ggccttgctc aagaagcagt
     8821 atcccgaggc cagggtgctg gtgcaccccg agtcgccgga gacggtggtg gcactggccg
     8881 atgtcgtcgg ttcgaccacc cagttgctga acgcggtgac gacgatggac gcccagacct
     8941 tcatcgtcgc caccgacaac gggctgatgc atcgcatgcg ccagctcgca cccaccaaaa
     9001 ccttgctcga agcgccgact gccggcgaca gcgccacctg caagagctgc gcgcactgcc
     9061 cgtggatggc gatgaacgcg ctgcagggcg tggtcgactg cctggaatcg ggccacggcg
     9121 agatccaggt cgccgagccg gtccgcagcc aggctctggg ctgcatcgac cgcatgctcg
     9181 atttcgtcaa ggcgcatccg accgcgacgc agaagcccgg gttcgttgcg cacatcggcg
     9241 cggcttgagc gcatccatgt tcgaaatcaa cgaagacctg gaagccgccc gcgcgcgcaa
     9301 cgtgcgcgat gcgctgatgg aagacatcgg ccagcgtgac tggacggccg aactggtgcc
     9361 cgccgggcag cgggtttcag cacgggtctt ggtgcgagag gaggcggtcc tgtgcggtcg
     9421 cgactggttc gacgcctgca ttgcggcgat ggaccctgcc gcccgggtcg actggcacgt
     9481 ggccgaaggt gcactgatgg gagccgacac ccgggtgtgc gacatcacct ccgatgcacg
     9541 cgccctgctt tcggcagaac ggccggcgct caatttcctg cagttgctgt cggccaccgc
     9601 gacgctgacg cgccgccacg tggatgcgat cgccggtgca tcgccgaacc cgcgcggctg
     9661 cgtgatcctc gacacccgca agacactgcc gggtctgcgt caggcgcaga agtacgcggt
     9721 gcgcaccggc gggggccgca atcagcggct ggcgctgtgg cacggcatcc tgatcaagga
     9781 gaaccacatc gcggcagctg gcggcatcac cgccgtgctt cgcaacgcgg cggcactgaa
     9841 cgccggcgtc gacatccaga tcgaggtgga aacactcgaa cagctacgcg aggccttggc
     9901 ggcaggtgcg gtcagcgtgc tgctcgacaa cttcgatctg gatggcatgc gcgcggccgt
     9961 cgcgctgagc gcggggcgag ccctgctcga ggtgtcgggc ggcgtgcggc ttgaccaggt
    10021 tcgcgagatc gccgccaccg gtgtcgaccg gatctcgatc ggccggctga ccaaggatgt
    10081 cagggcgacc gactactcga tgcgggtggt gtcgaccctc tgagggatcg aacgaccatc
    10141 tggaccgcag ttccggtgct attccgggtc tcggtgggcc caggtcggcc ggacactcgg
    10201 gtttcagctc cacgggggag cggagaccgc catgagcacc gtgttcgatt cgatccacaa
    10261 ccactatgag cacgcggtgt tcgacgccgt gctcgccgca tccagtcgct tcagccacct
    10321 cagcgacccg cacatcatgg cggacgtggc ctgtgtggcg ctgaaccgcc tgccgccgcg
    10381 ctacatccgg cacgacgtcg atttctcgtt ctatctgacc gagcgcgagc gctcggaaaa
    10441 cgaaacggcg atcgccgaag cggtggaatt cgcattccag ttcgtgcagg ccaaggtggc
    10501 catgcgcgca aggcgctgag ccgtgccggc ctcagacgct cagtcgatgc gtcttgggct
    10561 tcctgccgac gcgcgtgcgt cgcatcaaca cggtcgggaa actcaccggc agcgcctggg
    10621 gaaagtcgcg gctgtaatgc agcccccggc tctcgtgccg catcagcgcc gagcggacga
    10681 tgagttcggc acagtcgacg aggttgcgca gttccagcag gtcgcggttg acgcggaagc
    10741 tgccgtagta gtcatcgatc tcgctgcgca gcagcttgat gcggtgcagc gcccgttcga
    10801 gccgcttggt ggtgcgcacg atgccgacgt agttccacat cagcagccgc aattcgtccc
    10861 agttgtgcgc gatgacgacc tgctcgtcgg cgttctcgac ctggctctcg tcccaggccg
    10921 gcagatgcgg ctccgggtgg tcgccgagtt catggatatc catcgcgcag gtgcggccca
    10981 gcaccacgca ctccagcagc gaattgctgg caagccggtt cgccccgtgc agtccggtgt
    11041 aggtggtctc cccgaccgcg tacaggccgg gcaggtcggt gcggccgtgc aggtcggtga
    11101 ccacgccgcc gcaagtgtag tgcgcggcag gcaccacggg aatcggctgc cgggcaatgt
    11161 cgatgcccag cgccaggcag cgcgcgtgga tcgtcgggaa atgctccttc aggaaggcct
    11221 cacccaggtg cgtggcgtcg agccacacgt ggtcgagtcc gtgcttcttc atctcgaaat
    11281 cgattgcccg cgccacgatg tcgcgcggtg ccagctccat gcgctcgtcg tgcgctgcca
    11341 tgaaacgtgt gccgtctggc aacttgaggt gtccgccctc gccgcgcaag gcctcggtga
    11401 tcaggaagct gcgctcctgc gggtggtaga ggcaggtcgg atggaactgg atgaactcca
    11461 tgttgccgat gcggcaaccg gcacgccagg ccatcgcgat gccgtcgccg gtggcggtat
    11521 cggggttgct ggtgtatcgg tagaccttgc cgacaccacc ggtggccagc accaccgcac
    11581 gcgctggcaa cgtctcgacg cgctgctgat cgatgtcgag cgcgtagaca ccgtagcagc
    11641 gcggctgctc gtcgcgcctg agctggcggg acgtgatcac gtcgaccgcc atccagcgct
    11701 cgcgcatgtc gatgttggga tgccgcctga cctcgtcgag cagcacgtcg tggatcgcct
    11761 tgccggttgc atctgccgcg tgggcgatgc gacgcaccgc atggccacct tctcgcgtca
    11821 ggtgcagacc gagcggacct tcggggtccg ccgaaaacgg cacgcctcga tccaccagcc
    11881 agccgatcgc ctcggcgctg tgctgggcga tgaatcgcgc cgtgtgctcg tcgaccagac
    11941 ccgcgccggc atcctgcgtg tcgcggacat gcgaatcgat gctgtcgtca tcgcccagca
    12001 cgccgacgat gcccccctgc gcccaggcgg tggccccctc gtccagaccc cgcttggcca
    12061 gaacgatcac cggatggtcg accgccaggt gcagggccac cgtcaatccg gccagacctg
    12121 caccgatgat caccaccgga agctgcgcac gcgccgggtc aatgagcggc atggcggttc
    12181 ggcacggatc gaaggtctga aatcatcgcc ggattctatc gatcagcccc cgttcgccgg
    12241 gctcgggcgg ccagcaccgc ctgcggtgcg aaggaatgct gtcgcaggga tggtgcagtg
    12301 cgagagactg tgccagcatg gcgagtcgcc gcgtcgaatc acgatgcacc gactccggca
    12361 acgccgccaa ccacaacgta ccggcacgag ggccccatga gcaactacac cagccgcacc
    12421 ggcagtccct acacggaccg tcccgcacca ccgccgatcg agaacgacgg ctgggaatcg
    12481 acgcgggccc agactgcgct ggccacccag atcatggatc gcgaaggcga tgcgcccccc
    12541 acttatcgcc aggagatcgg tcggccggtt ggcaacgatg ttcgcgagct gttcgtggcc
    12601 tgcgcgccgg gcgaggcgct gcagcagcag ttcgagcacc tgcatccgga attcattgca
    12661 gtgcacgatc tcgccacgcg ttcgtcgcgg cagttgctgc gtggcatcgc agcggccagc
    12721 aaccgtgcgg tccagaagct ggtgctgcgc cgccagggcg gtggtgcacc ggtggcgacg
    12781 atcgacttca tcgaggtccc cgtcagcgcg caggagacgc tgcgcatcta ctccaccgat
    12841 atcgacgaca ccaccgacgc cgcggcccgc catgcggtcg cgcgcacctt gctggcctac
    12901 agccagctcg gagtgatgat ggtgggcgcc atgccggccg agaacatcca cgccctgctg
    12961 aggccctggc acgaggaact catccgcagt ccctggctga accgcaatct gctgctgctg
    13021 ccgctgacca acaccccggc gctgacgacc aacggccaga acatggtccg cggcacctcg
    13081 atcacgctgc gcaccacgcc cgtggtcacg cgaccggccg atgcctggaa tttcatcaac
    13141 ggcacctggg gccggctgcg aggcgctgtt tcgcctctgg gcgccccggc tgcaccggct
    13201 cgcagcgtca cgacagcccg cgccgccaca tcgcaacctc cggtccagag ccaggctgca
    13261 gcgcgagtca gtgcgcagat cgacgccgga taccggccac cagccgcgcc ggacgatcct
    13321 tcaggcttcg cgcccaccaa cctgctgcct ttgcgaccga tgccggacgt gcgggccagg
    13381 ccggtgcctc tgcaggacga agtgctcgag gtctacgtgc atcaggtgtg cgaactcaac
    13441 ggcgtggtcg gctgctgcgt tttcgatatc ggctccgggc tgccggtggc acatggcggt
    13501 gccggcgccc cgccggagga gctggcgcgc catggaaggg ccttgctgtc ggcgatgggc
    13561 accaccagcc gggcactcgg cctcggccac acgctgccgg atgccgccat cacgctcgga
    13621 tcccatcacc tgctgctgcg ctcggtcgcc aagcacgcag gcatggcgct gctgacggtg
    13681 ctggaccgga cgaacgccaa cctgacgctt gcccgtcttc agatcgagcg gctcgatccc
    13741 ttgctggacc gctgagccgc tctcgacgag aagggacgaa catttcgtcg cgagcgggcc
    13801 gaggtgacat cgaggagcca agccgtacaa gcgtacggtg agcaccggag gggtcagatc
    13861 ggcccgcgca gcagaaagat tcccccttct cagtgcacga cgcggtcgaa gacgaactcc
    13921 ccgtccgcga cccccaccgg aatcacgtcc ttcgggccga acttgccctc gaggatcagc
    13981 ttcgcgaccg ggttctcgat gcgttgctgg atcgcgcgct tcagcggccg cgcaccgaac
    14041 accggatcga aacccgcctt ggccagttcc gccagcgcct gcggcgtcac ctcgagcttc
    14101 atgtccatct tctcgagacg ggactccagc accttcagct ggatgccggc aatcgattcg
    14161 atgtgccgat ggtcgagcgc atggaacacg acgacctcgt cgatgcggtt gagaaactcg
    14221 ggacggaaat gctgcttcac ctcgacccac accgcctcgc ggatcagttc gctgtcctgc
    14281 ccggccatct gcatgatctg gtgtgaaccc agattgctgg tcatcacgat cacgcagttc
    14341 ttgaagtcca cggtgcgacc ctgcccgtcg gtcaggcgtc cgtcgtcgag cacctgcaac
    14401 agcacgttga acacgtccgg gtgcgccttc tcgacttcgt cgagcaggag cacgctgtag
    14461 gggttgcggc gaaccgcttc ggtgaggtag ccgccctcgt cgtagccgac atagcccggt
    14521 ggcgcaccga tcaggcggct caccgaatgt ttctccatgt actcgctcat gtcgatgcgg
    14581 atcaggtgct cctcgctgtc gaacaggaaa cccgccagcg ccttgcaaag ctcggtcttg
    14641 ccgaccccgg tcgggccgag gaacatgaac gatccagtcg gccggttcgg gtcactcaga
    14701 cccgagcgtg agcggcggat cgcattggcg accgctgtga tggcctcgtc ctgaccgatc
    14761 acgcgctcat gcagacgggt ctccatctgc agcagcttct cgcgctcgcc ctgcatcagc
    14821 ttggacaccg ggatgccggt ggcacgcgca acgacttcgg cgatctcctc ggcgccgacc
    14881 atcgtgcgca gaagttgcgg cttgccgccc gccttggcct tgccctgctc cttcgattgc
    14941 gcctcggcga gcttcttttc cagctccggg aggcgaccgt actgcagctc ggcgaccttg
    15001 ttgaaatcac ccttgcgctt gaggtcctcg atctggaacc ggatgcggtc gatctcttcc
    15061 ttgacgtggg ccgagccctg cgccgcagcc ttctccgact tccagatctc gtcgagatcg
    15121 gcagcctcgc gctgcagctt ggtgatctcc tcctcgatca ggccgaagcg cttctgcgag
    15181 gcctcgtcct tctcgcggcg caccgcctcg cgctcgatct ggagctggat caggcggcgg
    15241 tcgagcttgt ccatcgcctc gggcttcgag tcgatctcga tcttgacctt ggcagcggct
    15301 tcgtcgatca ggtcgatcgc cttgtcgggc aggaagcggt cggtgatgta gcggtggctc
    15361 agctcggccg cggcgacgat ggccgggtcg gtgatctcga caccatggtg cacctcgtac
    15421 ttttcctgca ggccgcgcag gatcgcgatg gtggcctcga ccgaaggctc gccaaccagg
    15481 atcttctgga agcgccgctc gagcgcggcg tccttctcga cgtacttgcg gtactcgttc
    15541 agcgtggtgg cgccgatgca gtgcaactcg ccacgagcca gcgccggctt gagcatgttg
    15601 ccagcgtcga tcgcgccttc ggccttgccg gctccgacca tcgtgtggat ctcgtcgatg
    15661 aagacgatgg tctgtccttc gtcctgcgcc acttccttca gcacgctctt gagccgttcc
    15721 tcgaactcgc cgcggaactt ggcgccagcc agcagcaggg ccatgtcgag caccagcacc
    15781 cgcttgttct tcagcgagtc gggcacttcg cccgcgacga tgcgctgcgc cagaccctcg
    15841 acgatggcgg tcttgccgac gccgggttcg ccgatcagca ccgggttgtt cttgctgcga
    15901 cgctgaagca cctggatcgc acggcggatc tcgtcgtcgc gtccgatcac cgggtcgagc
    15961 ttgccctgac gcgcacgctc ggtgaggtcg agcgtgtact tcttcagtgc ttcccgctgg
    16021 ccttcgccct cggcgctgtc catcttctgg ccgccgcgca ccgcgtcgat cgccgcttcg
    16081 agcgccttgc ggttcaggcc gtgttccttc accagcggac cgatggccgt cttgctgtcg
    16141 gccactgcga gcaggaacat ctcgctggcg atgaactggt cacctcgctt ggtcgactcc
    16201 ttctctgcgg cctgaagcaa ggctccaagg tcgcggctcg gctgcacctg ctcaccgccc
    16261 tgcacctgcg gcagcttctt cagctccgcc tcgagcgcac cttgcagctt ggccgtgttg
    16321 acgccggcgc gggcgagcag cgccttcggc ccgtcagcct gcgcgagcat ggccgacagc
    16381 acatgcaccg gctccatgta agggttgtcg cggccgttgg ccaggctctg tgcatcggcc
    16441 aaggcttcct ggaacgtggt ggtcaatttg tcaagacgca tggaaaggtc ctcctattcc
    16501 ctgcaatctg agggggtacc tgcgcatttc aaggcatggc ttcgggcggc aattgatcaa
    16561 gatcaatccc tccgaactgc aacgcgcagt accgcctcgg gccacacatc ggtcggaatc
    16621 gcgagaaact ccgtgcatga atccgacacc gaccaccgcc gcactggctg gcatcttttc
    16681 ggccctggcc tggcctctgc tgtggccctg gttgagcgaa tcgaccgctt cgagcagtgt
    16741 ctggctggtc gtgggaaccc tgctgttgat cgccttgcct gcccacgcat tcgtcgtcgg
    16801 gttcaagcgc aatccgctgc ccggtgccag agcgatcgac acggcactgc tcaagcgcat
    16861 cggagcgtgg ctgctggccg cagcactgac cagcttcatc atgagcctgc ttcccccgac
    16921 gtgacgcgct cgcaagcgcc gcgcgtcgag tccggctccg atccgaaggc tgcaggacct
    16981 cagtcctccc gcaggctcaa ccgcagagga acgctgccct ggctgcggtc ctccaacgga
    17041 cgcagcagca gcgacgtcat ttcgggactg agcgcgaaat cgaactggcc gttgctcttg
    17101 cgcttcaggc gcacatagcc actctcccgt tcgaagtcgt tgcggatgat cagcgtgatc
    17161 acctgctcgc acgtgctgat gaacacccgg aagcggaatc ggtctgcagc cagttcatcc
    17221 tgtatgccgc gaagccagca ggcttccctc ggcgctctgc ggtggttcga cggcagcggc
    17281 acgaacaccc gggttgcagc gtcatgcgac gcgtgcacca ccagttcgtc gctgcgggcc
    17341 agcaccgcga catgggcggg cgaagcgaac gcctgatcgt agagaaccac gagcgtgtct
    17401 tcctgccagg tggcgactcc aagcccggtg cgcaccccga agatcagtcc gttgacattc
    17461 aggtcgctcg agttctcgga cacgccgcgc gactgcacga tcgccgtgag gacgggcagc
    17521 atgcgctccg gcgacggatc tgcggccgcc agaatggaca ggatctcagg cgacagtgcg
    17581 gtgtgaaaag gcgtcggatc tccgatctgg agcagatccg acccggtcgt gtcgagcccg
    17641 ccgggcgagg tccagcgcaa cagttcacag gcaccatcgc tgccggagga agccagcagc
    17701 agagagaccg gcttcgcatc actgaagggg ccgaggctga cgtcgagcag cgaaaggagt
    17761 tcgccgttgt tcgtcacgcc tgcatggttg gcctggagga attcgagaag tcgcgacacc
    17821 gaaccggcat cgccggtaca ggctgcgacc gcaccgggcg cgattcgaac cagcttcaac
    17881 gcgggctctt cgatgggctc atcgtgatgc tcggtcagca gctcacccag gctgatctgc
    17941 tgcctgcgcg gcggaatctg caccgaagag accgtgtcgg ccagcaggta gaccgagcct
    18001 ttgtgcttcc aacctgcgca gtagctcatc gaaggaccgg cccggcaggg cggtcaggcc
    18061 agcagcgtct tcgcaacgcg gaatcccatg gcgcagcaga gcagtgcgcc ggccaggtgc
    18121 gcgaagctgt gcgccaccgc ccagccaaag tcgccacgct gcagcagcgt cagcgactcg
    18181 ccggtgaatg ccgagaatgt cgtcaacccg ccgaggaatc ccgtcacgag cagcagccgc
    18241 atcgattcgt tcggcgtcct ggcgaaccac gccagcgcga tcccgatcag caggccaccg
    18301 acgcagttga ccaccagcgt accgagcggg aaaccggccc agcgcgcgtt gagccacagc
    18361 tggaccgccc agcgcagcac gccgccgact gccgatccga gcgccacggc catcaactgc
    18421 gcccaaggca cggactggtt catcggtctg gctcggtggc cggatcaggc tcgccctgct
    18481 gccagcgcgc catcgcctcg tcgtcgctga cccgggcatc gacccagcgt gagccctccg
    18541 gagtgctctc cttcttccag aacggcgcct gcgtcttcag gtagtccatc aggaactcgc
    18601 aggcccggaa tgcgctgcca cgatgtgagg aggccaccgc caccagcacg atctgatcct
    18661 gcggctgcag caggccgaca cggtggatca cgcgcacggc gcggatgtcg aagcgcctcc
    18721 gggcggccga gatcatcgat tcgatcgact tctcggtcat gccgggatag tgctcgagtt
    18781 ccatcgcgct gacgccgctg ccatcgttgc ggtcgcgcac acagccgacg aaggtggcga
    18841 ccgcgccgac accgggatcg gccgaacgca aggccgcgac ctcggcgctc aggtcgaaat
    18901 cggcggtctg gatgctgacg ctcatgctgc tgtcccgctc aggcctgtgc tcagccgccg
    18961 gtcactggtg gaaagaaggc aacttcggca tcggcgccga caggctcgga ctcatcgcac
    19021 agcaactggt tcagcgcgct gcgcagggat cgctcacgcg acaaggcggt tgcgtgggcg
    19081 gcgctggatg caatcagccg gtcacgcaac tcgccgatcg tggcgccagc cggaacctcg
    19141 atcgattcac cagcgcccag cacctcgcgg atcgaagcga aatagcgcac ctgcacgttc
    19201 atgacagcaa ctccgcgaat ccgaggtact gcaccatgtc accggagcga atggcctgcc
    19261 ccgaagggtt gtcgaccagc ccatcgcccc acacggccga cgtcagcacg cccgagctct
    19321 ggttggcaaa caggtcgagt ccaccactcg aattgcggcg cacacgcagg aactcgcgac
    19381 gcctgtcggg cttgggccag tcgaagtcag cgcgcatcgg aaggctccgc agttgccagt
    19441 cgactgcgcc ttgcaggtgc agcagcaccg ggcgcacgac gagcaggaag gtgatgaaac
    19501 tggacaccgg gttgcccggc aggccgatga agtaagccgc gcccgaatcc gcagactcgc
    19561 cgcttcgcaa gggctcgcgc ctgacttcac caaacgccag cggcttgccc ggcttgatcg
    19621 ccatctgcca cagcgtgacg cgaccttcag cctcgacggc gggccggatg tgatcttcct
    19681 cgccggtcga cacaccaccg ctggtgacga tcaggtcgtg ctcctgcgcc gcacgacgca
    19741 gcgcttcgcg ggtggcatcg agccggtcgg gcacgatgcc gaaatcggtg cagtcgcaac
    19801 ccagcgcctg gaccagcgcg cgcagcgtga agcggttgga gttgtagatc gcaccgggct
    19861 tcaagggctc gcccggcatg accagttcgt cacctgtaga gaacaaggca acccgcgggc
    19921 gccgcgccac cgacaggctc gctgcgccca cggacgccgc cagcccgagc gcctggggcg
    19981 tcagtcgcgt gcctttcggc agcacgaccg aaccagcgag aacgtcttca ccgcgacggc
    20041 ggatccactg acccggactc ggcatggcat cgatgcgaac agcaccgcca tcaagcgcga
    20101 cgcactgctc ctgcatcacg accgcatcgg cacctgccgg gacctgggcc cccgtgaaga
    20161 tgcgcgcagc cgtgcccggc accagagggg tcccggccac gccagcggga attcgctggc
    20221 tgaccggcaa ggcgatgccg accgcagaca catccgcgca tctcatcgca tagccgtcca
    20281 tcgatgtgtt gtcggccggc ggcacatcga ccaccgatcg gacttcctcc gccagcacgc
    20341 ggccaagcgc atcgaacgtg gagacctgct gcgccctcac gacgccgaaa ggctggacgg
    20401 cctgcgtcag gcggtccagc gcttcgtcga gaccgagcag cggcgcacgg gcggccggcg
    20461 cagcggcatc accgatcggg ggagtcgagg tggggtggtt ggtactcatg gcgagccgaa
    20521 tcggacaaga ggaagtttgc caccgcgccc acgtcgttga ggtcgagcac cggcaggtcg
    20581 gtgggcaccg gcagggtagc gctgtcggtg cacacggcaa cgatgaacgg gtcgtgcgga
    20641 tactgcaccg ggttgccggt ttccgcccgc catacctcga ccttgtgcag atcggcgtgc
    20701 ttgaacccct ccaccagaac ccagtcgcag gagctcaatt cggcgatcaa ctcgtgcacc
    20761 gtgggctcgg cgatcgccgc gaactcgcga atcacggcaa gccgacgatt cgaggcgacg
    20821 accacttcgg acgcacccgc ccgccgatgc cgccaggagt ccttgcccgg atgatcgatg
    20881 tcgaaagcgt gatgcgcatg cttgacgacc gacacccgca gccctttctc gcgcaacaga
    20941 gcgatcagct gttcgacgag cgtcgtcttg cctgaaccgg aatagccgca gaaaccgatg
    21001 accttcattc ggtgaacgtc agcgggccgg gaaacggtca cacgttggcg ctgaggtacg
    21061 ccttgaccgc ctgggtatcg accggcatca ccacgaagcg cttgggcaga tcctcgatgc
    21121 cgcgcatcgc ggcgggccga tcgggctgca cgccgatggc ttcgacgatg gtctcggcga
    21181 acttggctgg cagcgccgtc tcgagcacca gcataggcac cccccaatcc aggtgctcgc
    21241 gaccgacctt caggccgtct gccgtgtgcg tatcgatgat cacgccgaag cggcgatagg
    21301 tgtcccggat cgtggcgagg cggtcggcgt gggtgctggt gcccgacacg aaaccgaagc
    21361 tcgcagtgtt cgcgaactca tcgtcactga cggtgaacga gcccttctgc tcgacctcgg
    21421 tcttgaacaa ctgcttcgtg cgcgcaccgt cgcggcccag cagatcgaag acaaagcgct
    21481 cgaagttcga ggccttcgaa atgtccatcg acgggctcga cgtctcatgc gtttcggcgc
    21541 tgccgcgcac ccggtaggtg ccggtgcgga agaactcgtc cagcacgttg ttttcgttgg
    21601 tcgcgagcac caggtagcgg atcggcagac ccatcatccg cgcgacatgg ccggcgcaga
    21661 tgttgccgaa attgcccgac ggcaccgcga agctgacctg ctggtcgttt gaccgcgtgg
    21721 cctggaaata cccggcgaag tagtagacga cctgcgccag cagccgcgcc cagttgatcg
    21781 agttgacggt gccgatgtgg tggcgccgct tgaaatcgag gtcgttcgac accgccttga
    21841 cgatgtcctg gcaatcgtcg aacaccccgt cgatcgcgat gttgtggatg ttggcgtcct
    21901 gcaggctgaa catctgcgcc tgctggaagg
//
LOCUS       DAOORV010000054        21595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2967_length_21595_cov_7.672748, whole genome shotgun sequence.
ACCESSION   DAOORV010000054 DAOORV010000000
VERSION     DAOORV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21595
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2967_length_21595_cov_7.672748"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKV56_09050"
     CDS             complement(<1..685)
                     /locus_tag="PKV56_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68002.1"
                     /translation="MSDFLLETRDLTREFKGFVAVNQVNLRVRRGTIHALIGPNGAGK
                     TTCFNLLTKFLAPTSGRILFDGQDITDEPPARIARRGVIRSFQISAVFPHLTVLENVR
                     VALQRQLGTSFHFWKSERSLDGLNGRALELLREVDLEAYAALPAVELSYGRKRSLEIA
                     TTLAMDPKLMLLDEPTQGMGLEDVDRIRQLIKKVAAQRTVLMVEHNMSVVASIADTIT
                     VLQRGATLAE"
     gene            839..1282
                     /locus_tag="PKV56_09055"
     CDS             839..1282
                     /locus_tag="PKV56_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOX68003.1"
                     /translation="MTGTVRLHRVLRAPPERVYRAFLDADAMAKWLPPCGFTCKVHQM
                     DPRVGGTFRMSFTNFATGNGHSFAGEYLELVPHERLRYTDRFDDPNLPGEIQVTVALT
                     QVLCGTELSIVQEGIPDVIPLEMCYLGWQESLAQLATLVEPEIPG"
     gene            1429..2088
                     /locus_tag="PKV56_09060"
     CDS             1429..2088
                     /locus_tag="PKV56_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68004.1"
                     /translation="MIDLYTSATPNGWKASIALEELGLPYTVHPIKLGLQEQKQPEYL
                     KINPNGRIPTIVDRDEGDFAVFESGAILIYLAEKTGQLMPQAMKPRSQVIQWLMFQMG
                     GVGPMQGQANVFFRYFPEKIPSVIERYQKETKRLFTVLDTRLKDHEYLVDDYSIADIA
                     NWSWVRIHDWAGVAVDDLPNLQRWLALMAARPACQRGVEIPEPFMPKEVVDYAKKILA
                     S"
     gene            2183..2569
                     /locus_tag="PKV56_09065"
     CDS             2183..2569
                     /locus_tag="PKV56_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68005.1"
                     /translation="MEIKGPNPKPLTGIAMAWPAIDGKAFAIRLQTPDGDLDLELNDT
                     TLKQLLQALLQSSAACAPKRTDLQPITKSELADGVQLPASGIDVMQIEGEAERRRLVM
                     RVGTIDLNLMIGSRTTAKALANALMA"
     gene            2629..3612
                     /locus_tag="PKV56_09070"
     CDS             2629..3612
                     /locus_tag="PKV56_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68006.1"
                     /translation="MASTPLSHHDILALVEPFTRRGRHVDLPASDRLERRLRFKPVEH
                     AGGTSGEPALLDTLQLDSFESGGFRLTRTLSRCDSLQATLQVSGPDPAALLAQVEAVA
                     PPHQFVTGPGFVIARSYSLQPVSDRATDRRSVLILTRAVVQFDDLALTLSVPAIRRVS
                     ADLWLAPASGGTSGTGHESALDLPEDLLAVLGWNWARLIKKRDGWESKLRLRGDLARR
                     TQGAETALDRVAAHLVQTLSQPPAQFHDRHLKARWWAAFRRAIPLLTPVSLLLAILLL
                     PSLDVGESPGLWLMLFHVPTALIAISFCLQELPQYEIPPLPRRSRAAAWRA"
     gene            3661..4248
                     /locus_tag="PKV56_09075"
     CDS             3661..4248
                     /locus_tag="PKV56_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68007.1"
                     /translation="MNERITQLLDQMAALEDELRTALHEQESRMFFQIKGKRVEFESS
                     VKEAHRKLKTNFFRWLVAYRPQNLITGPVIYGMAIPLLLMDACVSFYQWACFPIYGIT
                     KVRRSDYLVFDRHQLGYLNFIEKFHCTYCEYGNGLMAYMREILARTEEYFCPIKHARK
                     VIGTHARYRRFLDYGDAADYEARLEAFRVGLGQVK"
     gene            4254..5783
                     /locus_tag="PKV56_09080"
     CDS             4254..5783
                     /locus_tag="PKV56_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HOX68008.1"
                     /translation="MESSDLRIRRVAIDTWRENVVYLHRDCAVVRAAGFQALSKVMVR
                     ANGAVISAVLNVVDDSCIVEPGELGLSEDAFARLDVQAGHAAQVSPAEPPVSLPALHR
                     KIAGERLSREDMHALIEDVARARYSKIELAAFVVATNGYDLERDEVLYLTEAMIATGR
                     RLDWQQAVRGGPVVDKHCIGGIPGNRTSMLVVPIVTAHGMLCPKTSSRAITSPAGTAD
                     TMEMLARVELPFDRLQKIVRDTNGCLAWGGSADLSPADDILISVERPLGIDSPGQMVA
                     SILSKKIAAGSTHLVLDIPVGPTAKVHSMPAAQRLRKLFEYVASSLNLSLDVMITDGS
                     QPIGNGIGPVLEARDVMRVLRNDPAAPADLREKSLRLAGRVIEFDPDVRGGDGWRIAR
                     DILESGRALAQMEAIIDAQGRRAELPALGELTHEVCAEQAGSVGAIDNLRLARIARLA
                     GAPQVSGAGVDLLRKAGDPVVAGQSLYRIHACYPADLSFARQLASRDSGYTISAAGAA
                     R"
     gene            5780..6685
                     /locus_tag="PKV56_09085"
     CDS             5780..6685
                     /locus_tag="PKV56_09085"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOX68009.1"
                     /translation="MMRDTTAPGCVLAFDDERDMATALAGALDVPLALIERHRFPDGE
                     LKLRLPATLPACVVLLRSLHQPNEKLVELLITVPAARELGAQRVLLACPYLAYMRQDI
                     AFTPGEAVSQRHIGALLASQFDAVVTVDPHLHRIEALDEVMPGCRAVSLSAAALIGDW
                     VARHVSRPLLLGPDEESAQWVTEAARACSPAPDHGWCVKQRHGDRDVVVTLPRTPDCR
                     GREVVLIDDMASTGRTLMAAAQLALAAGALSVDVAVTHALFVGDAMAELKAAGVRHVW
                     STDCVRHASNAISVVPLLANALRAA"
     gene            complement(6749..7075)
                     /locus_tag="PKV56_09090"
     CDS             complement(6749..7075)
                     /locus_tag="PKV56_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68010.1"
                     /translation="MSYAYPAEKFAAARQALTLSSTQGEPQAVVSAFHECRAGLHRMN
                     RSKLDENARSWIYSLECFMDTEGLSVPAGESAWTVKATSLSSEQKLEIAQLVDDLANW
                     FKSQTS"
     gene            7233..9293
                     /locus_tag="PKV56_09095"
     CDS             7233..9293
                     /locus_tag="PKV56_09095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOX68011.1"
                     /translation="MKHIRIAALAATAAFLASPASAEDVPTFGLPLGFSVEKMDRTAD
                     PRKDFARYASGRWLDAAQIPGDTVRISPLDQMSRRVDAQVLAILDEAARTAGSATRGT
                     PRQQVGDHHAAGLDVKRITELGISPLAPEFARIDAIDGPRSLAAMLARLNLLLNDPVV
                     AGAGVATDPTDRTRYTVFAAEPALTVPSNEVYLDSAMAPVRAGLLRYIADVFVLAGVP
                     SETASAQAKKILEIETRVATKKLSPVDMADPAKLFTRMRYAELKSLAPAVDWDTYLGE
                     LGLAPPNDVVVANREALRERSLLLADLPLEDTKAYLRWELLRRAVPYLPPAFIEANFA
                     FSRVLYGPLDIPPRDRLVAREMATRLGQPLSQLYVDKHFSAQAKKAAEDLVLRVRMQF
                     RQRLLANRWLTPETRRYALEKLDKVSIKVGYPDAWIDHSTVDIRRDDYLGNALRINEF
                     RARRDLARLGQPVAQDLFADPGATLPIVINAAYEPSRNGIEIPAAFMQPPIYDPKADA
                     AVNFCALGAVIGHEITHGFDSQGRLYDADGNVRDWWAPQDVKRFNAQADKLVAQANAF
                     QVLPGLHANGALAVGENLADLGGVSLAYAALRAHLHESPKENVKIDGLSPDQRCFIAW
                     AQVWADKAHEGWLRQVTANDPHPPGRYRASAPARHERGFYKSFGIHKGDPMWLDPKDR
                     VTLW"
     gene            complement(9578..10300)
                     /locus_tag="PKV56_09100"
     CDS             complement(9578..10300)
                     /locus_tag="PKV56_09100"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOX68012.1"
                     /translation="MSAGSKPAETIARRRWFNLTLASVLVAGLVACGGGGSDSSPVMG
                     VDVNGSSTINAEVLAAQLVTYPLAPLAATEVQSLLFMREEEQLAHDVYALSATLWAPP
                     IFANISASEATHSAAMKALIDRYQLVDPLAGLAEGTFKTPAFQALFDALVATSRVSLV
                     DALKVGVEIEELDIRDITAQKVGIDNADILMVYDHLLRGSRNHLRAYMKVLTQQGGTY
                     VPQYISQAEFDAIVNSAIETGP"
     gene            complement(10297..10686)
                     /locus_tag="PKV56_09105"
     CDS             complement(10297..10686)
                     /locus_tag="PKV56_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68013.1"
                     /translation="MRTVRTVVIASLLGLSLGIACAQGGGGKGPGSGAGPMGAGPDAG
                     APGMGMGSGTGAGRAQWGSDFTPGWTLMSQQERNEHRDRMRSMKSYEECHAYQAQHHE
                     QMVARAKERGGAAPAQPRRDACAGLKK"
     gene            10992..11348
                     /locus_tag="PKV56_09110"
     CDS             10992..11348
                     /locus_tag="PKV56_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOX68014.1"
                     /translation="MSSQPVSYRDLTQSVSASLASLKSGTPDVMKSFSELGRAATANG
                     VLDKKTKELIALALSVAARCDPCIGFHMQTLVKLGATRQEIDETLGVTTYMGGGPSVM
                     YAASAIAAFEEFSSAR"
     gene            complement(11523..11705)
                     /locus_tag="PKV56_09115"
     CDS             complement(11523..11705)
                     /locus_tag="PKV56_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68015.1"
                     /translation="MAFLDMIAVVASKEVPERSENLALRNLINQFMNGQISVGRIGSA
                     SANTSSLKYILTPRAS"
     gene            complement(11759..12571)
                     /locus_tag="PKV56_09120"
     CDS             complement(11759..12571)
                     /locus_tag="PKV56_09120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX68016.1"
                     /translation="MTRATGARRNSDRMPHEEWDSLCANVAGRLRGSAEHPAAAPVRE
                     ELIACAQDLEVLRAALLQEQGHLSRIEAELNEARATLAVAHARERDAQYMAQHDSLTD
                     LPNRRHFCRRLDDALSTAPTRPPTMAVLFLDLDDFKPINDAHGHETGDELLRIVAQRL
                     RRMVRDGDIVCRLGGDEFACLLSQPRDRAELSRLARQLLEAIAAPLQLGGIEIRVWPS
                     IGIAVCPNDGDTAITLLRRADAAMYRAKRGRLGYAFFDSRSDVTASETGGLH"
     gene            13021..14166
                     /locus_tag="PKV56_09125"
     CDS             13021..14166
                     /locus_tag="PKV56_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX68017.1"
                     /translation="MNPSTTAPSNPEATDEDLARQARPGHGIPSQDPTPGAQVALDPE
                     EAEREASSVLMGGGVVAGAATGAAIGIMMAGPVGVVVGASMGAVAGALGGAAAGSAMN
                     PEESGVAGAAPDHIVRIHIEDSGGAGRPVVLIHGWPLTAQAWELQVAALRADGYRVVA
                     YDRRGFGRSDKPASDYSYDTLVDDLQRVMDRCGLQDVTLVGFSMGGGEVARYIARHGE
                     SRLHSVVFAAAVPPYLLKTADNPEGPLAPEKAQQKRQSLEQDRGAYFDQFTRDFFSAH
                     GTLQVSESQRAEAIALCQQSAQHAALACMDSFGTTDFREDLKKVSVPTLVIHGDADAI
                     VPIAGSGPRTHRAVSHSQLVTVSGAPHGLNVSHAREFNDALLSFLRT"
     gene            14354..15406
                     /locus_tag="PKV56_09130"
     CDS             14354..15406
                     /locus_tag="PKV56_09130"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOX68018.1"
                     /translation="MTTLAYAAQDATTPLAPFSIERRAPGPDDVQIDILYCGVCHSDL
                     HTARNEWKNTLYPSVPGHEIVGRVSAVGANVTKFKLGDMAGVGCMVDSCLHCASCGEG
                     LEQYCDNGFTGTYNGPVFGGENTFGGYSSRIVVKQKFVLRISHKDNLQAVAPLLCAGI
                     TTYSPLRQWGVEKGQKVGIVGLGGLGHMGVKIAAAMGAHVVLFTTSASKVEDARRFGA
                     AEVVISTDPQAMAAHQSSFDFILNTVAAQHDLDPFIALLKRDCTMTLVGAPEHPHPAP
                     NVFGLIFKRRRLGGSLIGGIRETQEMLDFCAEHGIVSDIEMIAMKDINTAYERMLKSD
                     VKYRFVIDMATLQATP"
     gene            15403..16287
                     /locus_tag="PKV56_09135"
     CDS             15403..16287
                     /locus_tag="PKV56_09135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX68019.1"
                     /translation="MTSSAHEPPLPSSTLNALEAALDQSHEVKAKVEACADDLATAND
                     IVKERITEGETLLPAAEALENSVAVELQVQECADDLKQVTDNLAQGLGQVKEVEEALS
                     RSHEALRESQTALVTSREAEQLASRRAMHDQKTGLPNRTLFDDRLAQAIAGAERHGWA
                     LAVMFLDLDQFKAVNDTHGHAAGDTVLAIVAERLLNHMRDEDTVCRNGGDEFLYLLID
                     PAGRDNVARIAAQLRTTLARPIAVSGLELAITPSIGIALYPDHGKTADVLVAHADAAM
                     YRSKQKRSPCEFYQPAGS"
     gene            complement(16326..18461)
                     /locus_tag="PKV56_09140"
     CDS             complement(16326..18461)
                     /locus_tag="PKV56_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX68020.1"
                     /translation="MSYDPPVSTIHGPCVIVVIVDITDRVQAQKALQDLAAGLEQRVL
                     ERTAELASARDAEAAANRAKSTFLANMSHEIRTPMNAIIGLTHLLAREVTQPSQRARL
                     DKIGTAVRHLLQVINDILDLSRIDAGKITLENTEFSLDELVGRAIEMVGDRAREKGLE
                     LVLDTDRSPERLRGDPTRLLQALINLLANAVKFTASGWVRLGIATLQRDEHQAVLRFE
                     VQDTGEGIDAHVLPNLFNAFEQADSATTRRFGGTGLGLALTRHLAQLMGGDVGVESTP
                     GVGSTFWFTARLDLGTPQAIRSEPSLLAGRRALLVDDLSESLQAVGDRLRMLGLDVDE
                     FDSPQAALAHVSEAAVTARSYDVLVLDWRMGPPDGVQLLASLRTMMDPVPPPALLVTA
                     HDEAAMWEQARAAGFDAVLLKPITASGLQDALLSALRPRGTRDPQPEPTEQNRSEDSV
                     ESKLRTLHSGRRVLLAEDNPINREVAVELLSAVGLVVETSENGREAVDQALRQAFDLV
                     LMDMQMPELDGLEATRRIRAAGRVTLPIIAMTANAFDEDRRACLAAGMNDHVAKPVDP
                     ERLYAALLQWLPVPVAGQSVPAPTPALPALAGDVRPLLQRLADVEGLDVQQAMRCVGG
                     QLPILRRLLDLFVRTYQQGAGELDAHAAHSLRGACATVGATDLQAALVHYESCLAAGI
                     DVGELRQQADLIHASLLALTASLRSELDR"
     gene            complement(18512..19558)
                     /locus_tag="PKV56_09145"
     CDS             complement(18512..19558)
                     /locus_tag="PKV56_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX68021.1"
                     /translation="MVNPAHPGRALLPPLTDDELISLFHHLFDPCLDAVLLTHETGVV
                     RAANPAACEVFGAPASAICEQTASRGRAALVDEPDPRLQPLLAEREANGRARGELRMR
                     RLCGETFDAEVSSFRFHDLGQSSDFILMVRDLRAQRAAELRAVESEQRLAFALQAADI
                     GDWSLDLSTGIARRSLHHARCFGDDDADAPWSFTTFLSRVEAADRDSLEQSLRQAQDS
                     DGIHDIEFRVRWPDGSLHWLWAKSRFYFDSQRRPLKVAGIVADITERRRTEGELRERE
                     ALLSTLTNRARVGMVMVDSERRYVFANLAYAEVLGLEQSAIAGKRIADVLPGVFESQI
                     QPKLELAFAGNAVT"
     gene            complement(19884..20276)
                     /locus_tag="PKV56_09150"
     CDS             complement(19884..20276)
                     /locus_tag="PKV56_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX68022.1"
                     /translation="MKGYVQDIEAIAIGNEDFRKVLYTASNCQLVVMALKPKEEIGAE
                     VHKLDQFFRVEEGHGEAVLDGVRTAIQAGSAVVVPAGARHNIINTGTESLKLYTIYSP
                     PNHRDGVVHHTRADAEADSEHFDGKTSE"
     gene            complement(20923..>21595)
                     /locus_tag="PKV56_09155"
     CDS             complement(20923..>21595)
                     /locus_tag="PKV56_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HOX68023.1"
                     /translation="TAQGLGDCIDCTLCVQVCPTGIDIRDGLQNECIGCAACIDVCGD
                     VMDKMGYPKGLIRYSTENGVALGWTRLQMLKRALRPRVLIYGAVLLSASIAFATSMAL
                     RSPFKFDVVKDRGTLARVVDDGAIENVYRIQIMNRTEARQTYRVTASGIDGLVVSATT
                     ASAGPASVASLVVSLRLAHADAQLLQGASTPLMFEVAVLDSPSSSAAGSAAVQREKST
                     FFVPR"
BASE COUNT         3776 a         7255 c         6993 g         3571 t
ORIGIN      
        1 cttcggccag cgtcgcgccg cgctgcagca cggtgatggt gtcggcgatg ctggcgacca
       61 cgctcatgtt gtgctcgacc atcagcacgg tgcgctgcgc cgcgaccttc ttgatgagct
      121 ggcggatgcg gtccacgtcc tcgaggccca tgccctgcgt cggctcgtcg agcagcatca
      181 gcttcgggtc catcgcgaga gtcgtcgcga tctcgagcga gcgcttgcgc ccgtagctca
      241 gctccaccgc cggaagcgcc gcatacgcct ccaggtcgac ctcgcgaagc aactcgagcg
      301 cccggccgtt gagcccgtcc agcgaacgct cggacttcca gaagtggaac gaggtgccga
      361 gctgtcgctg cagcgcgacg cgcacgttct ccagcacggt caggtgcgga aacaccgccg
      421 agatctggaa cgaccggatc acgccgcgcc gcgcgatgcg ggccggcggc tcgtcggtga
      481 tgtcctgccc gtcgaacagg atgcggcccg aagtgggcgc gaggaacttc gtcagcaggt
      541 tgaagcacgt cgtcttgccc gcgccattgg gcccgatcaa cgcatggatg gtgccgcgtc
      601 gcacacgcag gttgacctgg ttcacggcga cgaagccctt gaattcacgg gtcaggtcgc
      661 gggtctcgag gaggaagtcg ctcatgaggg cgggagttta gggggcgccg cgggcctgtc
      721 cgaatcggtc gccatggggt ttctgctcac gctggatgcg cgagcgaatg cccggtgaac
      781 ctcgtacagt gaagcgcccc gggttctgat cgctcggcgc accccaggag gatcaaccat
      841 gacaggtacc gtgcgactgc atcgcgtgct ccgggctccc ccggagcgtg tctaccgggc
      901 tttcctggac gccgacgcca tggcgaagtg gctgccgccc tgcggtttca cctgcaaggt
      961 ccaccagatg gacccgaggg tcggcggcac gttcaggatg tcgttcacga acttcgccac
     1021 cggaaacggc cactcgttcg caggcgaata ccttgaactg gtcccgcacg agcggctgcg
     1081 ctacacggac cgattcgacg acccgaacct gcccggcgaa atccaggtga cggtggcgtt
     1141 gacccaggtc ctttgtggca ccgagctcag catcgttcag gaaggcatcc ccgacgtcat
     1201 tccgctggag atgtgctacc tcggctggca ggagtccctc gcgcaactcg ccacgctggt
     1261 cgagcccgag attcctggct agggcctgta cgccggccgc ccgcgcccgc gcggacttct
     1321 ggtcgcctgc agcggacgcg ttacgcaggt ataacgccga ggcattgcgg cctgcgcatc
     1381 gttgatcggc cggccggtca cctcccaccc acttcacgcg cccacaccat gatcgacctc
     1441 tacacctccg ccacccccaa cggctggaaa gcctcgatcg cgctggaaga actcggcctg
     1501 ccctacacgg tgcatccgat caagctcggc ctgcaggagc agaagcagcc cgagtacctg
     1561 aagatcaacc ccaacggacg cataccgacc atcgtcgatc gcgacgaggg cgacttcgcg
     1621 gtgttcgagt cgggcgcgat cctgatctac ctggccgaga agacgggcca actgatgccc
     1681 caggcgatga agccgcggtc gcaagtgatc cagtggctga tgttccagat gggcggcgtc
     1741 ggtccgatgc agggccaggc caacgtgttc ttccgctact tccccgagaa gatcccgtcg
     1801 gtgatcgagc gctaccagaa ggaaacgaag cgcctgttca ccgtgctcga cacgcggctg
     1861 aaggaccacg agtacctggt cgacgactac agcatcgccg acatcgccaa ctggtcgtgg
     1921 gtgcgcatcc acgactgggc cggcgtcgcc gtcgacgacc tgccgaacct gcagcgctgg
     1981 ctggccctga tggccgcgcg accggcctgc cagcgcgggg tcgagatccc tgagccgttc
     2041 atgccgaagg aagtggtcga ctacgcgaag aagatcctcg cgagttgaac cgtcccccaa
     2101 cgtcgccacg tgcccgagtt cgtggcacga tcccgggttg tctgaaaccc agagcaacac
     2161 tcaacccacc acaggccaga tcatggaaat caaaggaccc aaccccaagc ccctcaccgg
     2221 tatcgccatg gcttggccgg cgatcgacgg caaggccttc gcgattcggc tgcagacgcc
     2281 cgatggcgac ctcgacctcg agctcaacga cacgacgctg aagcagctgc tgcaagccct
     2341 gctgcagtcg tcggctgcct gcgcgccgaa gcgcaccgat ctccagccga tcaccaagag
     2401 cgaactggcc gatggcgtcc agttgcccgc gagcggcatc gacgtgatgc agatcgaggg
     2461 cgaagccgag cgccggcgcc tggtgatgcg cgtcggcacg atcgacctga acctgatgat
     2521 cggctcgcgc accacggcca aggcactggc aaatgcactg atggcctgag cgcatcaggc
     2581 agcgcgcggc agcgatcatc ggcctgattc ggcaccgacg aggcggccgt ggcgtcgacc
     2641 ccgctgtcgc accatgacat cctcgctctg gtcgagccgt tcacgcgccg aggccgccat
     2701 gtcgacctgc ccgcgagcga ccgcctggag cggcgcctgc ggttcaagcc agtggagcac
     2761 gcgggcggca cttcaggcga gccggcgctg ctcgacaccc tgcagctcga cagcttcgaa
     2821 tcgggcggct tccggctgac gcgcacgctg agccggtgcg acagcctgca agccacgctg
     2881 caggtcagcg gccccgatcc cgccgcattg ctggcgcagg tcgaagccgt cgcgccaccg
     2941 caccagttcg tgactggccc gggtttcgtg atcgcgcgca gctattcgct gcagccggtc
     3001 tcggaccggg ccaccgacag acggagcgtc ttgatcctga cccgtgccgt ggtccagttc
     3061 gacgacctcg ccctgacgct cagcgtgcca gcgattcgcc gggtgtcggc cgacctgtgg
     3121 ctggcacctg caagcggtgg cacgtccggc acgggacacg agagtgcgct cgacttgccg
     3181 gaagacctgc tcgccgtgct gggctggaac tgggcacggc tgatcaagaa acgggacggc
     3241 tgggaaagca agctgcggct gcgcggcgat ctggcccggc ggacccaggg cgccgagacc
     3301 gcgctcgacc gggtcgcagc gcacctcgtc cagacgctgt cgcagccgcc cgcgcagttt
     3361 cacgaccggc atctgaaggc gcgctggtgg gcggccttcc gccgcgcgat tccgctgctg
     3421 acgccggtca gcctgttgct cgcgatcctg ctgctgccca gcctcgatgt cggcgagtcg
     3481 cccgggctct ggctgatgct gttccatgtg ccaacggcgt tgatcgcgat cagcttctgc
     3541 ctgcaggaat tgccgcagta tgagattccg ccgttgcccc ggcgctcgcg cgcagccgcg
     3601 tggcgagcgt gatgaaggcc ggatcgcgag acgcgaccgg cggcgaacta tcctctccgc
     3661 atgaatgaac gaatcaccca gctcctcgac cagatggccg cactcgaaga tgaactccgc
     3721 accgcgctcc acgagcagga gagcaggatg ttcttccaga tcaagggcaa gcgcgtcgag
     3781 ttcgaaagct cggtcaagga agcacaccgc aagctgaaga cgaacttctt ccgctggctg
     3841 gtcgcctacc gcccacagaa cctgatcacc gggccggtca tctacggcat ggccataccg
     3901 ctgttgttga tggatgcctg cgtcagcttc taccagtggg cctgctttcc gatctacggc
     3961 atcaccaagg ttcgccgcag cgactacctg gtgttcgatc ggcaccagct cggctacctg
     4021 aacttcatcg agaagttcca ttgcacctac tgcgaatacg gcaacggcct gatggcctac
     4081 atgcgcgaga tcctggcccg cacagaggag tacttctgcc cgatcaagca cgcccgcaag
     4141 gtgatcggca cccacgcacg ctaccgccgt ttcctggact acggcgacgc cgccgactac
     4201 gaggcccggc tggaggcctt ccgcgtgggt ctgggtcagg tcaaatagcc acgatggaat
     4261 catcagacct ccgcatacgc cgcgtggcga tcgatacctg gcgcgagaac gtggtctacc
     4321 tgcatcgcga ttgcgccgtg gtgcgggcgg ccgggttcca ggcgctgtcc aaggtgatgg
     4381 tgcgcgccaa cggggcggtg atcagcgccg tgctgaacgt cgtcgatgac agctgcatcg
     4441 tcgagcccgg cgaactgggc ctgtccgagg acgcctttgc aaggctcgat gtccaggccg
     4501 gtcatgcggc acaggtatcg ccggccgagc cgccggtgtc gctgcccgcg ctgcaccgga
     4561 agatcgccgg cgagcggctg tcacgcgaag acatgcacgc gctgatcgag gatgtcgccc
     4621 gcgcccgcta ctcgaagatc gaactggcgg ccttcgtcgt cgcgaccaac ggctacgacc
     4681 tcgagcgcga cgaggtgctg tatctgaccg aggcgatgat cgccacgggg cgccggctcg
     4741 actggcagca ggccgtgcgc ggcggaccgg tggtcgacaa gcactgcatc gggggcatcc
     4801 cgggcaatcg cacgtcgatg ctggtggtgc ccatcgtcac ggcccacggc atgctgtgcc
     4861 cgaagacctc gtcacgcgcg atcacgtcgc cggcgggcac cgcggacacg atggagatgc
     4921 tggcccgggt cgagttgcca ttcgatcgcc tgcagaagat cgtgcgcgac accaacggct
     4981 gcctggcctg gggcggcagt gccgacctgt caccggccga cgacatcctg atctcggtgg
     5041 aacgcccgct gggcatcgat tcaccggggc agatggtcgc ctccatcctg tcgaagaaga
     5101 tcgccgccgg ttcgacgcac ctggtgctcg acatcccggt cggccccacc gccaaggtgc
     5161 actcgatgcc ggccgcgcag cggctgcgca agctgttcga gtacgtcgcc tcgagcctca
     5221 acctcagcct cgacgtgatg atcaccgacg gcagccagcc gatcggcaac ggcatcgggc
     5281 cggtgctcga agcgcgcgac gtgatgcgcg tgctgcgcaa cgatcccgcc gcgccggccg
     5341 acctgcgcga gaagtcgctg cgactggccg gccgcgtgat cgagttcgac cccgacgtgc
     5401 gcggcggcga tggctggcgc atcgcgcgcg acatcctcga atccggccgc gcattggcgc
     5461 agatggaggc gatcatcgac gcccagggcc ggcgggccga actgcccgcg ctgggcgagc
     5521 tgacgcacga ggtctgcgcc gagcaggcgg gctcggtcgg cgccatcgac aacctgcgcc
     5581 tggcccgcat cgcccggctg gccggcgcgc cgcaggtcag cggcgccggg gtcgatctgc
     5641 tgcgcaaggc gggcgatccg gtcgttgcag gccagtcgct ctatcgcatc cacgcctgct
     5701 acccggccga cctcagtttc gcgcgtcagc tggccagccg cgacagcggc tacacgatct
     5761 ccgctgcagg ggctgcccga tgatgcgcga cacgacagca cccggctgcg tgctggcctt
     5821 cgacgacgaa cgcgacatgg ccaccgcact ggccggcgcg ctggacgtgc cgctggcgct
     5881 gatcgagcgc catcgcttcc ccgacggcga attgaagctg cgtctgccgg ccacgttgcc
     5941 cgcgtgcgtg gtgctgctgc gcagcctgca ccagcccaac gagaaactgg tcgaactgct
     6001 gatcaccgtg ccggctgcac gcgaactggg agcgcagcgc gtgctgctgg cctgccccta
     6061 tctcgcctac atgcgacagg acatcgcgtt cacgccgggc gaggcggtca gccagcgcca
     6121 catcggcgcc ctgctcgcca gccagttcga cgcggtggtg accgtggacc cgcacctgca
     6181 ccgcatcgag gcactggacg aggtgatgcc aggctgccgg gccgtgtcgc tgtcggctgc
     6241 agcgctcatc ggagactggg tggcccgaca cgtgagccgg ccgctgctgc tcggtcccga
     6301 cgaggagtct gcgcaatggg tcaccgaagc cgcgcgagcc tgctcgcctg cgcccgatca
     6361 cggctggtgc gtcaagcagc gccatggcga cagggacgtc gtggtcaccc tgccgcgcac
     6421 gccggactgc cgtggccggg aggtcgtgct catcgacgac atggccagca ccgggcgcac
     6481 gctgatggcg gcggcacagc tcgcgctggc agccggcgcg ctgtcggtgg acgtggcggt
     6541 gacccacgcg ctgttcgtcg gcgatgcgat ggccgagctg aaggccgccg gcgtgcgcca
     6601 tgtctggagc accgactgcg tgcgacacgc cagcaatgca atcagcgtgg taccgctgct
     6661 cgccaacgcc ctgcgcgcgg cctgatcgcc gatcggcgtc gcgcgggcgt cgatgcgggt
     6721 gcgcgccgat gcacgccccc gctcgtggtc aggacgtctg gctcttgaac cagttcgcca
     6781 gatcgtccac cagttgggcg atctccagtt tctgctcgct ggacaggctc gtcgccttga
     6841 cggtccaggc gctttcgccg gcgggcaccg acaaaccctc ggtgtccatg aagcattcga
     6901 gcgagtagat ccaggaacgc gcgttctcgt ccagcttcga acggttcatc ctgtgcaggc
     6961 cggcgcggca ttcgtgaaaa gcgctcacca cggcctgagg ctcgccttgg gtactcgaca
     7021 gcgtcagggc ctggcgagcg gcagcgaact tctcggctgg gtaggcatag ctcatcaaca
     7081 actcccgttt tggaaaccgg cgattttcgc tcacgggtgc tcgatgattc cggtgcaacg
     7141 cctcggggcg tccgagttga cgggcggcag aatgaacggc ggagcgcagc cggatcgagc
     7201 gccgacaagc aacgagccac aggggattca cgttgaaaca cattcgcatc gccgcgctcg
     7261 cggcaacggc cgcattcctg gccagccctg cttccgccga ggacgtgccc acgttcggtc
     7321 ttccgctcgg gttctcggtc gagaagatgg accgcacggc ggatccacgc aaggacttcg
     7381 cgcgctacgc ctcgggtcgc tggctcgatg ccgcgcagat ccccggagac acggtccgca
     7441 tctcgcccct cgaccagatg tcgcggcggg tcgacgcgca ggtgctggcg atcctggacg
     7501 aggccgctcg gacagccggt tcggcgacgc gtggcactcc ccgacagcag gtgggtgacc
     7561 accacgccgc cgggctcgac gtgaagcgga tcaccgagct gggcatctcg ccgctggcgc
     7621 cggagttcgc gcgcatcgac gcgatcgatg gccccaggtc gctcgccgcg atgctggcgc
     7681 ggctcaacct gctgctcaac gatcccgtgg tggctggcgc cggggtggcc accgacccga
     7741 ccgatcgcac ccgctacacg gtcttcgccg ccgagcctgc actcacggtg ccctcgaacg
     7801 aggtctacct cgacagcgcg atggcgccgg tacgcgcggg gctgctcagg tacatcgccg
     7861 acgtgttcgt gctggcaggc gtgccgagcg agaccgcgag cgcgcaggcg aagaagatcc
     7921 tggagatcga gacccgcgtc gccacgaaga agctctcccc ggtagacatg gccgatccgg
     7981 ccaagctctt cacgaggatg cgctacgcgg agctgaagtc gctcgcaccc gccgtcgatt
     8041 gggacactta cctgggcgag ctggggctcg caccgccaaa cgatgtcgtg gtggccaatc
     8101 gcgaggcgct tcgcgagcgc agcctgctgc tggccgatct gccgctggaa gacaccaagg
     8161 cctatctgcg ctgggaactg ctgcgacgtg ccgtgcccta cctgccgccg gccttcatcg
     8221 aggcgaactt cgcgttctcg cgcgtgctgt acggaccact cgacatcccg ccgcgagaca
     8281 ggctcgtcgc cagggagatg gccacccggc tcgggcagcc gctttcgcag ctctacgtgg
     8341 acaagcactt cagcgcgcag gcgaagaagg cagccgaaga cctggtgctg cgcgtgcgaa
     8401 tgcagttccg ccagcgactg ctggccaacc ggtggctcac tcccgagacc cgccggtacg
     8461 ccctggaaaa gctggacaag gtgtcgatca aggtgggcta ccccgacgcg tggatcgacc
     8521 actcgacggt ggacatccgc cgggacgact acctgggcaa cgcactgcgc atcaacgagt
     8581 tccgcgcccg ccgcgacctg gcccggctgg gtcagcctgt cgcgcaggac ctgttcgcgg
     8641 acccgggggc caccctgccc atcgtcatca acgccgccta cgagcccagc cgcaacggca
     8701 tcgagatccc ggccgcgttc atgcagccgc cgatctacga cccgaaggcg gatgccgcgg
     8761 tgaacttctg cgcgctgggc gccgtgatcg gccacgagat cacgcatgga ttcgactcgc
     8821 agggacgtct ctacgatgcc gatggcaacg tgcgcgactg gtgggcaccg caggatgtga
     8881 agcgattcaa cgcccaggcc gacaaactcg tcgcccaggc caacgccttc caggtgctgc
     8941 cgggacttca cgccaacggg gcactggccg tgggcgagaa cctcgccgac ctgggcggcg
     9001 tgtccctggc ctacgccgcg ctgcgggcgc acctgcacga gagcccgaag gagaacgtga
     9061 agatcgacgg gctcagcccg gaccagcgct gcttcatcgc ctgggcccag gtctgggcgg
     9121 acaaggccca cgaaggctgg ctgcgccagg tgacggcgaa cgacccgcat ccgccgggca
     9181 gataccgtgc gagcgcccca gccaggcacg agcgcggctt ctacaagtct ttcgggatcc
     9241 acaagggcga cccgatgtgg ctcgatccga aggatcgcgt gacgctctgg taggccacta
     9301 gccgcgctga acgagacaag ccgaaggttt ccatgcttct ccggtttgct ctgctggatc
     9361 acaagagcgc gacctcaagg gccacttcag ctcgccggtc agcaagctcg gttcaacatg
     9421 cacgcctgca tgctcgattc aggcgagacc catgatccgg cgtccgcggc gactctcgtc
     9481 aacgcacggc cgaagtgggc actcagaccg cggctgatcc gactttccgg cagcggccag
     9541 gaagtcgttc cctgctaggc cagggtcagc agcgggatca aggaccggtt tcgatggcgg
     9601 agttcacgat cgcatcgaac tcggcctgac tgatgtactg ggggacgtac gttccgccct
     9661 gctgggtcag aaccttcatg tatgccctca gatgattgcg cgagccacgc agcaggtggt
     9721 cgtagaccat caggatgtcc gcattgtcaa tgccgacctt ctgcgccgtg atgtcgcgga
     9781 tgtccagttc ttcgatctcc acgccgacct tgagtgcgtc gaccaggctg acccggctgg
     9841 tggcgaccaa ggcatcgaaa agcgcctgga acgccggggt cttgaaggtg ccttcggcca
     9901 gccccgccag cggatcgacg agttggtacc tgtcgatcaa ggccttcatg gcggccgaat
     9961 gcgttgcctc gctggcactg atgttcgcga agatgggggg tgcccacagc gtggcgctga
    10021 gtgcatagac gtcgtgcgcc agttgctctt cctcgcgcat gaacagcagg ctttgaacct
    10081 cagtggcagc cagcggcgcc agcggatagg tcaccagctg tgccgcaagc acttcggcgt
    10141 tgatcgttga gctgccgttg acgtcgacgc ccatcacggg ggaactgtcg cttccaccac
    10201 caccacaggc gacaagtcca gcgaccagca cgctcgccaa ggtcaagttg aaccaccggc
    10261 gccgcgcgat ggtttcggcc ggcttgctgc cggcgctcat ttcttcagcc cggcgcaggc
    10321 atcacgacgg ggctgggcag gtgctgcgcc accacgctcc ttggcacgtg ccaccatctg
    10381 ctcatggtgc tgtgcctgat aggcatggca ttcctcatag cttttcatgg agcgcatgcg
    10441 gtcgcgatgc tcgttgcgct cctgctggct catcagcgtc caacctgggg tgaagtcgga
    10501 tccccattgc gccctgccgg cgccagttcc gctacccatg cccatgcccg gcgctcctgc
    10561 gtccggccct gcccccatcg gacccgcgcc cgagcctgga cccttgccgc caccgccctg
    10621 ggcacacgcg atgccaagcg agaggccgag cagggatgca atcacgaccg ttcgaactgt
    10681 tctcatgttt cgctcctgga atgacgatgc tggagatcga cattaatcag atgggcaggt
    10741 ggtgtccttg cgattgcgca atgaatacga cggaactcca gtaacagaaa gtgatcgcct
    10801 cgggccgctg catggcagcg caggccggcc ggttgatcag gccagttggt tgaagatgct
    10861 tacggggccg gattggtatc cgtgccatcg caccatgagc ctgcagcgca gttgcgcctg
    10921 gcgaccttcc ctttggcccg ggtccgaccc taaccagaag caccattcat cagatcccat
    10981 ggaggtcaac aatgtccagt caaccagtca gctaccgcga cctgactcaa tcggtttccg
    11041 caagcctcgc aagcttgaaa tccggcacgc cggatgtcat gaagagcttc agtgagctgg
    11101 ggcgcgcggc caccgcgaat ggcgttctcg acaagaagac caaggagttg attgctctgg
    11161 ccctcagcgt cgccgcgagg tgcgatccct gcatcggatt ccacatgcag accctggtca
    11221 agctgggtgc cacacggcaa gagatcgatg agacgctggg agtcacgacc tacatgggcg
    11281 gtggtccgtc ggtgatgtat gcagccagcg caattgctgc cttcgaggag ttttcctctg
    11341 ctcgctgagc gggctggatc tgctgccggg agtacgccct gctgatgcga ttgcgcttgc
    11401 cggtagtgaa tgtcggggtg cccgagatga ctctgcgccg gcatcccgcg ccaatctgga
    11461 tcgttgccat cggttcactg cgtgactgct tgcgcaagag atgaaacagc tggccaggtc
    11521 gatcagcttg ccctcggtgt caggatgtat ttcaagcttg aagtgtttgc cgacgccgac
    11581 ccgatgcgcc cgaccgagat ctgcccgttc atgaattgat tgatgagatt gcgaagcgcc
    11641 aggttctcgc ttcgctcagg aacctccttg gatgcgacca cggcaatcat gtcgaggaag
    11701 gccattcccc tttgaacgca gttcatctcc aatgagtaag accgctcttc gttgacgatc
    11761 agtgcagacc gcccgtttca gacgccgtca catcggaccg actgtcgaag aacgcgtagc
    11821 ccagccggcc gcgtttggct ctgtacatcg cggcgtccgc gcgccgcagc aaggtgatgg
    11881 cggtgtcgcc atcgttcgga cacaccgcga tgccgatgct cggccacacc ctgatctcta
    11941 tgccgcccag ttgcagcggc gccgctatgg cttcgagcaa ctgacgggcc aggcggctga
    12001 gctcggcacg gtccctgggt tgcgaaagca ggcaggcgaa ctcgtcgcca cccaggcggc
    12061 agacgatgtc gccgtcacga accatgcgcc gcaggcgctg ggcgacgatg cgcagcaact
    12121 cgtcgcccgt ttcgtggccg tgggcgtcgt tgatgggttt gaagtcgtcc aggtccagga
    12181 acagcacggc catcgtcggc ggacgcgtgg gcgcggtgct cagcgcatcg tccagcctgc
    12241 ggcagaaatg gcggcggttg ggcagatcgg tgaggctgtc atgctgggcc atgtattgcg
    12301 catcgcgctc gcgggcgtgc gccaccgcaa gcgtggcacg ggcctcattc agctcggctt
    12361 cgatgcggct caggtggccc tgctcctgaa gcagggccgc acggagcact tccaggtcct
    12421 gcgcgcaggc gatgagttcc tcgcgcacag gcgctgcagc gggatgctct gcgctgccac
    12481 gcagccgtcc ggcaacgttg gcgcaaagcg aatcccactc ctcgtggggc atgcggtcgg
    12541 aatttcgccg cgcacctgtg gcgcgggtca tgacgagcgt ccggtgtgca agggcccgag
    12601 tcgggcggag gccgccggac cctgacccac accgggcccg tcacggcagt cggcgctgcg
    12661 aggtctttcc gccgtggcgc caagtctcat cagtctgtag aagatccgaa cggtgaggtg
    12721 tgggctcatg gtgcagtcct tcgatcgtgg atccagatgg gcgacgcgct cagcttgagg
    12781 gagaggcccg cggagttctg tgcgctgccg caccgatggg cgcaagcgca gcgtgcagtc
    12841 tccaatccgc cactgcatgg tgcggatgca tctccagaac cagaagctga aaccgctcac
    12901 tgatcgttgt cctacagcgt ctcggcctgc aggacggcgt gggcagtgcg gcaggggccg
    12961 tacaaggatc tgttgcccga gcgcctgctc gctgcagcac caatttccct aggagaggcc
    13021 atgaatccat ccaccaccgc gccgtccaac cccgaagcca ccgacgaaga cctggcccgg
    13081 caggcccgcc caggccacgg catcccgtcg caggacccga cccccggggc acaggttgcg
    13141 ctggaccccg aggaagccga gcgcgaggcc agctcggtgc tgatgggcgg cggcgtggtg
    13201 gccggtgcgg ccacgggcgc cgccatcgga atcatgatgg ccggcccggt gggggtggtg
    13261 gtcggggcct cgatgggtgc cgttgccggc gcactcggtg gcgctgctgc aggatccgcc
    13321 atgaaccccg aggaatcggg cgtcgccggc gccgcacccg accacatcgt gcgcattcac
    13381 atcgaggaca gtggtggtgc cggccgaccg gtggtgctga tccatggctg gccactgacg
    13441 gcccaggcct gggagctgca ggtggcggcg ttgcgcgccg acggctaccg cgtggtggcc
    13501 tatgaccgaa ggggcttcgg tcgttcggac aagcctgcat cggattacag ctacgacacc
    13561 ctggtcgacg acctgcaacg cgtgatggac cggtgcggac tgcaggacgt gacgctggtg
    13621 ggcttctcga tgggcggcgg cgaagtggcc cgctacatcg cgcgtcacgg cgaatcgcgt
    13681 ctgcacagcg tggtgtttgc cgcggccgtg ccgccgtatc tgctgaagac ggccgacaac
    13741 cccgaagggc cgctcgcgcc cgagaaggca caacagaagc ggcaatcact cgagcaggat
    13801 cgcggtgcct acttcgacca gttcacccgg gacttcttct cggcccacgg cacgctgcag
    13861 gtcagcgagt cgcagcgcgc cgaagcgatt gccctgtgcc agcagtcggc gcagcatgcg
    13921 gcgctggcgt gcatggactc cttcggcacg accgacttcc gcgaagacct gaagaaggtg
    13981 agcgtgccca cgctggtgat ccacggcgac gcggacgcga tcgtgccgat cgcaggctca
    14041 gggccgcgca cccaccgcgc ggtgagccac agccagctgg tgactgtgag cggtgcgccg
    14101 cacgggctga acgtgtctca cgcccgggaa ttcaatgacg cactgctgtc gttcctgcgg
    14161 acatgacctg ccgtgaccgc tgacagggca tgccgccaac gcgctggtgc aggctgctct
    14221 gctccggtga tgatgcgcga cgacgtcgcg gtcggcggca gagcctgcct cgcgaccctg
    14281 ctgcgcgcag ttcacaccct gcatcatcaa tcaggccggt tcgcctgaac ccaccggaag
    14341 aggaagagct cccatgacca cactcgccta cgcggcccag gacgccacca ccccgctcgc
    14401 ccccttcagc atcgagcgcc gtgcgcccgg gccggacgac gtgcagatcg acatcctgta
    14461 ctgcggtgtc tgccactccg acctgcatac cgcacgcaac gagtggaaga acacgctcta
    14521 cccgtcggtg cccggccacg agatcgtcgg ccgcgtcagt gcggtcggtg ccaacgtcac
    14581 caaattcaag ctcggcgaca tggccggcgt gggctgcatg gtcgacagct gcctgcattg
    14641 cgcatcgtgt ggcgaagggc tggagcagta ctgcgacaac ggcttcacag gcacctacaa
    14701 cggcccggtg ttcggcggag agaacacctt tggcggttac tccagccgga tcgtggtgaa
    14761 gcagaagttc gtgctcagga tcagccacaa ggacaacctg caagccgtcg ctccgctgct
    14821 gtgcgcgggc atcacgacct attcgccact gcgtcaatgg ggtgtggaga agggccagaa
    14881 ggtcggcatc gtcgggctgg gtgggctggg gcacatgggc gtgaagatcg ccgccgcgat
    14941 gggcgcgcac gtggtgctgt tcaccacgtc ggccagcaag gtcgaggacg ccaggcgctt
    15001 cggcgccgcc gaagtggtga tctcgacgga tccgcaggcc atggcggcgc accagagctc
    15061 gttcgatttc atcctcaaca ccgtcgccgc ccagcacgac ctggacccgt tcatcgcgct
    15121 gctcaagcgc gactgcacga tgaccctggt cggcgccccc gagcatccgc acccggcgcc
    15181 gaacgtgttc ggcctgatct tcaagcgccg ccgcctcggc ggatcgctga tcggcggcat
    15241 tcgggaaacc caggaaatgc tcgacttctg cgccgagcac gggatcgtct ccgacatcga
    15301 gatgatcgcg atgaaggaca tcaacaccgc ctacgaacgc atgctcaaga gcgacgtgaa
    15361 gtaccgcttc gtgatcgaca tggccacgct ccaggcgaca ccatgacctc ttccgcgcac
    15421 gaaccacccc tgccctcgag caccttgaac gccctggagg cggccctgga ccagagccat
    15481 gaggtcaagg ccaaggtgga agcgtgtgca gatgatctcg cgacggccaa cgacatcgtg
    15541 aaggaacgga tcaccgaggg cgaaaccttg ctgcccgccg ccgaggccct ggagaacagc
    15601 gtggccgtcg aactccaggt gcaggagtgc gccgacgacc tgaagcaggt caccgacaac
    15661 ctggcgcagg gcctcgggca ggtcaaggaa gtggaagagg ccttgagccg gtctcacgag
    15721 gcactgcggg agtcccagac cgctttggtg acttcgcgcg aggccgagca attggcaagc
    15781 cgacgggcca tgcacgacca gaagaccggc ctgccgaacc ggacgctctt cgatgaccgg
    15841 ctggcccagg ccattgcggg cgcagagcga cacggctggg cgctcgccgt catgtttctc
    15901 gacctcgatc agttcaaggc tgtcaacgac acgcacgggc atgccgctgg agatacggtg
    15961 ctcgcgatcg tggcggagag gctgctgaac catatgcgcg acgaggacac ggtctgccgc
    16021 aacggtggcg acgaattcct gtacctgttg atcgaccctg cgggtcgcga caacgtggcc
    16081 cggatcgcag cccagctgcg cacgacgctc gcccgaccca tcgccgtcag cggtctggag
    16141 cttgccatca cgccgagcat tggaatcgcg ctctaccccg atcacggcaa gactgcggac
    16201 gtgctggtgg cacatgcgga tgccgccatg taccgatcca agcagaagcg cagtccctgc
    16261 gagttctatc aacctgcggg ctcctagccc accggctcga tgaccacgct cgcaggactg
    16321 acccctcagc gatccagttc agaccgcagc gatgccgtca acgcgagcag gctggcatgg
    16381 atcagatcgg cctgctgccg caactcgccg acatcgatgc ctgctgcaag gcagctctcg
    16441 tagtgcacca gcgcggcttg caggtccgtc gcgcccacgg ttgcgcaggc cccacgcaac
    16501 gaatgagcgg catgagcgtc gagttcgccc gcaccttgtt gataggtcct gacgaataga
    16561 tcgagcagac gccgcaatat aggcaactgg ccacccacgc agcgcatcgc ctgctgaaca
    16621 tccaggccct cgacatccgc cagacgctgc aagagcggcc ggacatcccc cgccagggca
    16681 ggcagcgcag gtgtcggcgc cggcacggac tgacccgcga ccgggaccgg cagccattgc
    16741 agcagcgcgg catacaggcg ctcgggatcg accggcttgg cgacgtgatc gttcatccct
    16801 gcggcaaggc aggcgcggcg gtcctcgtcg aaggcgttgg cggtcatcgc aatgatgggc
    16861 agcgtcacgc gaccggctgc acgtatgcgt cgcgttgctt ccagtccgtc gagttccggc
    16921 atctgcatgt ccatgagcac gaggtcgaaa gcctggcgca gggcctggtc caccgcctcc
    16981 cgaccgttct cggaagtctc gacgaccagg ccgaccgcac tgagcagttc gaccgccacc
    17041 tcgcgattga tggggttgtc ttccgcgagc agcacccggc gtccggagtg gagcgtccgc
    17101 aatttcgact cgactgagtc ctcgcttcgg ttctgctcag ttggctcggg ctgcggatcc
    17161 cgggtgcctc ggggccgcaa cgcgctcaac agcgcgtctt gaagccccga tgccgtgatc
    17221 ggcttcagca gcaccgcgtc gaacccggcg gcgcgcgcct gctcccacat cgccgcttcg
    17281 tcgtgggccg tcacgagcag tgccggcggc ggcacgggat ccatcattgt ccgcaggctc
    17341 gcgagcagct gcaccccgtc tggaggcccc atgcgccagt cgaggaccag cacgtcgtac
    17401 gaccgagcag tcaccgcggc ttccgaaaca tgcgccaacg ccgcctgcgg actgtcgaac
    17461 tcatcgacgt ccagaccgag catccgcagc ctgtctccca cggcctgcag cgactcgctc
    17521 aaatcgtcga cgagcagcgc gcgccgaccc gccagcaggg agggctcgct tcggatcgcc
    17581 tgcggcgtgc cgagatccag gcgcgccgtg aaccagaacg tgctgccgac accgggcgtg
    17641 ctctcgacgc cgacgtcgcc gcccatcagt tgcgcgaggt gacgtgtcaa cgccagaccg
    17701 aggccggtgc cgccgaaccg ccgcgtggtc gcgctgtcgg cctgctcgaa ggcattgaag
    17761 agattcggca gcacgtgcgc gtcaatgcct tcgcccgtgt cctgcacctc gaagcgcagg
    17821 accgcctggt gttcatcgcg ctgcagcgtc gcgatgccga gcctcaccca gccggaggcc
    17881 gtgaacttca ccgcgtttgc cagcaggttg atgagggcct gcaaaagccg cgtcgggtct
    17941 ccacgcaacc gctcgggcga acggtccgtg tcgagcacga gttccaatcc cttctcccgg
    18001 gcccggtcgc cgaccatctc gatcgcgcgt ccgaccagtt catcgagcga gaactcggtg
    18061 ttctccagcg tgatcttgcc ggcgtcgatc ctcgagagat cgaggatgtc gttgatcacc
    18121 tgcagcagat gcctgacggc ggtaccgatc ttgtccaggc gggcgcgctg cgacggttgc
    18181 gtgacctcgc gagccagcag gtgcgtcagg ccgatgatcg cgttcatcgg cgtgcggatc
    18241 tcgtggctca tgttggccag gaaggtgctc ttggcccggt tggccgccgc ctcggcatcc
    18301 cgcgcgctcg ccagttcggc ggttcgctcc aggacgcgct gctccaggcc ggcggccagg
    18361 tcctgcaagg ccttctgggc ctgcacgcga tccgtgatgt cgacgatcac gacgatcacg
    18421 cagggcccgt gaatcgtcga aaccggcggg tcataggaca cagcgaaaac gcgatctgca
    18481 tcccagcccg cgcggcgagg cacgttcaat tctaggtcac cgcattgccg gcgaaggcca
    18541 gctcgagctt gggttggatc tggctttcga aaacccccgg cagaacgtcg gcgatgcgct
    18601 tgccggcaat cgccgactgc tccagtccga ggacctctgc gtaggccagg ttggcgaaga
    18661 cgtagcgcct ttcgctgtcc accatcacca tgcccacccg cgcgcggttg gtcagcgtgc
    18721 tcaacagcgc ctcacgttcc cgcaactcgc cctcggtacg ccttcgctcg gtgatgtcgg
    18781 ccacgatgcc ggccaccttc agcggcctgc gctgcgaatc gaaatagaag cggctcttgg
    18841 cccacaacca atgaagcgac ccatccggcc aacgcacgcg gaactcgatg tcgtggatgc
    18901 catcgctgtc ctgcgcctgt cgcaggctct gttccaaact gtcgcgatcg gcagcctcga
    18961 ccctcgagag aaaggttgtg aagctccagg gcgcatcggc atcgtcgtca ccgaaacacc
    19021 gcgcatggtg cagggatcgg cgagcgatgc cggtggacag gtccaggctc cagtcgccga
    19081 tatcggcagc ctgcagagcg aaggccagtc gctgctcgct ctcgaccgca cgcaactcgg
    19141 ccgcccgttg tgctctcaga tcccgcacca tcaggatgaa atcggatgac tgccctaagt
    19201 cgtggaaccg gaacgacgaa acctcggcgt cgaacgtttc gccgcacagg cgacgcatgc
    19261 gcaactcacc gcgagcgcgg ccgtttgcct cgcgctcggc aagcaacggt tgcaagcgcg
    19321 gatccggttc gtcgaccagg gcggcgcgcc ctcgcgaagc agtctgctca cagatcgccg
    19381 aagccggagc accaaacact tcgcaagctg ccgggttcgc cgcgcgaacc acaccggtct
    19441 cgtgcgtcag cagcacggca tcgaggcagg gatcgaacag gtgatggaac agcgagatca
    19501 actcgtcgtc ggtcaacggc gggaggagtg cccggccagg atgtgcggga ttcaccatgg
    19561 ttcagtggtc ttggatcggt gcctgaaggc actgtgccac gcggtgtcga ttgaaggtgt
    19621 agacaccccg ctcaaggcaa ggccaagcgc gcatccgcag atgccccatc caatgttcag
    19681 gtacccaacc aagcaagcgt cacctcgtct cgcagggccg gccacccgca cgcttcgccg
    19741 cagagaaacg gcaagccccg actggacgac ttgccgaccg ttgcaagagg gtcctgagcc
    19801 gagtcggtga gttgaagatc tcgtccgatc aggcctgcct tttcatcgca gccggcctga
    19861 gcgaagcggc gcgttcctcc gcatcattcc gaggtcttgc cgtcgaagtg ctcgctgtcg
    19921 gcctctgcgt ctgcccgtgt gtgatgaacc acgccgtcgc ggtggttggg cggcgaatag
    19981 atggtgtaca gcttcaggga ctcggtgccc gtattgatga tgttgtgcct ggcgccagca
    20041 gggacgacca cggcggaccc ggcctgaatc gccgtccgga cgccgtcgag caccgcctcg
    20101 ccgtgcccct cctcgacccg gaagaactgg tccagcttgt ggacctcggc accgatttct
    20161 tccttgggct tcagcgccat gacaacgagc tgacagttgc tcgcggtgta cagaaccttt
    20221 cggaagtctt cgttgccgat ggcgatggcc tcgatgtctt gcacgtaacc cttcatgcct
    20281 gctccagtag ttggcgcggg ccggtgtcat cgcagtgctc gatggggctt cgagtgacct
    20341 gatctgcgag tcgccgacgt cgtcccgggc aaggtagtcc cgcagccggg ggccgtctgt
    20401 gcggagacga acggaagtgg gcggcgccgc gatcattcaa cgctgcaaaa aacgaagggc
    20461 ccgacttgtg atcgggccct tgctttgtct gcgctacgcg tgcggcggcg aataaaccgg
    20521 ccgaacccgt ctgagttttt gattttgttt ttttggtctg gcggtgggca cagtcgccag
    20581 cgaacccgtc tcggcgcagc gcctgttaac agggaaattt acagggaatt tgcgcggaaa
    20641 tcagcttcaa cccatggctg aagcgcccta ctccccattc agatcaatgg gttacgggcg
    20701 atttccagct cgagccgaat tgcggaacag ggaattaaca ggggacggca catcgatcga
    20761 aagtcgcggc cctgccctca ctgcagaggc gcagtcaggc ctggctcgcc aactcgcccg
    20821 agagaatcag gttttccgca tcgcgccgaa ctcgccacct gtgcagtcaa agcggtggct
    20881 tgactggaga gccacccgaa atccgctagc agtgtcagcg aatcaacgag gcacgaagaa
    20941 ggttgatttc tcgcgctgaa cggccgcaga accagcagcg ctggagcttg gcgagtccag
    21001 taccgcaacc tcgaacatga ggggcgtcga tgcgccctgg agcagttgtg catcagcatg
    21061 agccagtcgc aaactgacca ccaaggatgc gacgctggcc ggaccggcgc tcgccgtcgt
    21121 tgcggacaca accagaccgt cgattccact ggcggtcacg cggtaggtct gtcgagcttc
    21181 ggtgcgattc atgatctgaa tccggtacac gttctctatt gcaccgtcgt cgaccacacg
    21241 ggccagggtg cctcggtcct tgaccacgtc gaacttgaac ggactgcgaa gcgccatgct
    21301 ggtcgcaaac gctatgctgg cagacaacag cactgcgcca tatatcagca cgcgcggccg
    21361 aagggcgcgc ttgagcatct gcaggcgcgt ccaccccagc gcgacaccgt tctcggtgga
    21421 atagcggatc aggcccttgg gatagcccat cttgtccatc acatcgccgc agacgtcgat
    21481 acaggcagcg caacctatgc actcgttctg cagaccatct cgaatgtcaa tgccagtggg
    21541 gcagacctgc acacacagcg tgcagtcaat gcaatcgccc aggccctgcg cggta
//
LOCUS       DAOORV010000055        21462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_2993_length_21462_cov_8.109637, whole genome shotgun sequence.
ACCESSION   DAOORV010000055 DAOORV010000000
VERSION     DAOORV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_21462_cov_8.109637"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKV56_09160"
     CDS             complement(<1..424)
                     /locus_tag="PKV56_09160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOX68024.1"
                     /translation="MHLNTAKTGANTPARPNKIVSAAEAVRLIHDGDTVATGGFVGIG
                     FAEGIAIALEERFLAIEAETGSGSPRNLTLVYAAGQGDGQDRGLNHFGHAGLVKRVVG
                     GHWGLVPKLQALALSGEIEAYNLPQGVISHLFRDIAAGK"
     gene            complement(547..804)
                     /locus_tag="PKV56_09165"
     CDS             complement(547..804)
                     /locus_tag="PKV56_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HOX68025.1"
                     /translation="MEQPRNPLHGLTLEAIVSALVEHYGWAGLAERIPVRCFTSDPSV
                     ASSLKFLRKTPWAREKVEGLYLFMLRDIRRAAPRAAKPEAG"
     gene            1026..1775
                     /locus_tag="PKV56_09170"
     CDS             1026..1775
                     /locus_tag="PKV56_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX68026.1"
                     /translation="MNAPVVLITGALAGIGRATALAFAREGARVVVSGRHDDAGAQLV
                     AELRALGAEAEFVRTDVRIEEEVRNLVDKTVARFGRLDVAVNNAGTEGQPGVVTETSA
                     EKYAGTFDTNVLGTLLSMKHEMRVMLAQGSGNIVNLSSVLGRVGIPGAAVYVASKHAV
                     EGLTKCAALEGAAAGVRVNAVSPGPVETGMLDRFTGGSEAAKAGFIAAMPARRASTAD
                     EIAQAILFVASDKSSLLNGQSLAIDGGYSSQ"
     gene            2044..2499
                     /locus_tag="PKV56_09175"
     CDS             2044..2499
                     /locus_tag="PKV56_09175"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HOX68027.1"
                     /translation="MKKLFLALVAVAALYVGWGQTEGLHQRAGVPDLETRAPATSRAD
                     DIRAAFSERDSGTQVTGKGVVTKLLADDSEGSRHQRFILKLASGQTVLVAHNIDLAPR
                     IDSLEPGDSVSFNGVYEWNAKGGVVHWTHHDPSGRHEAGWLRHSGQTYQ"
     gene            2602..3057
                     /locus_tag="PKV56_09180"
     CDS             2602..3057
                     /locus_tag="PKV56_09180"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HOX68028.1"
                     /translation="MSPTELQEFLHRSVPLSRAMGVAVEAITPDSVVLAAPFEQNINQ
                     HGTVFGGSSATLSVLAAWSLLHTRLMVEGIDVKLVVQRSAMEYEAPMAGRFTATASLP
                     PGAHWRHFVDAIRARGKARIQVLAVVGSSGPPAGRFQGEFVAVREAGSA"
     gene            3093..3506
                     /locus_tag="PKV56_09185"
     CDS             3093..3506
                     /locus_tag="PKV56_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX68029.1"
                     /translation="MKPNSLSKAIIGSLLFSIGLGAGFFAQTLTDSAQRVEHKRSDLV
                     GAPDMEVIVSTAEYKPGETIDLHLHHGAEAVYVIQGAKVQAPGKDATQIPTGVSLLNQ
                     RDVKHAGFTIVGDASLKLFTVHVVDKGKPLYEYAR"
     gene            complement(3635..4510)
                     /locus_tag="PKV56_09190"
     CDS             complement(3635..4510)
                     /locus_tag="PKV56_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOX68030.1"
                     /translation="MKRPWLMAVIVANLLALVALVFVYPHLMISPGTLVGAHAELATD
                     CFACHAALRGASSERCTACHATSDIGLRTTKGVPLPPAAGHVAFHQELIEQDCMACHS
                     DHQGPKLTQRSRKPFSHALLRAETRDRCESCHSAPKNNVHRDLKASCASCHSTDRWTP
                     ATFKHELLSQAVLAACEACHKAPTDNLHRKITGECSQCHTPKAWKPATFDHDKHFVLD
                     GDHNTACETCHTNSDFSRYTCYGCHEHTPAKVRAEHEEEGIRDFQNCVKCHRSANEEP
                     TREGSGGSGDRRERD"
     gene            complement(4507..5094)
                     /locus_tag="PKV56_09195"
     CDS             complement(4507..5094)
                     /locus_tag="PKV56_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68031.1"
                     /translation="MTSMKARLISREVVPFLLAFAALGASALLIDGILHLLDAVWIGR
                     YLGIPGTLLIIGSFGYSMRKRKLISSGQPATLLRLHERMAWTGSLLVLVHAGIHFNAI
                     LGWLAVWAMGINIASGLVGKYLLKRSRQRLDEARQRMQQQGLSVDTLEDRLYWDSLTF
                     DAVKQWRVIHFPISLAFAVLAVAHIVAIFLFWGWK"
     gene            complement(5091..6326)
                     /locus_tag="PKV56_09200"
     CDS             complement(5091..6326)
                     /locus_tag="PKV56_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX68032.1"
                     /translation="MTAPELLALICATLLLQVVAGAGVALWRRRRGAESPPEAAIANA
                     APAPAGAWPGWRDFRVARREFEDAAQSQCSFYLEPADGQPLPAFRPGQFLTFSLRVAG
                     DAVPVRTVTRCYSLSDCPDPSHYRITVKRVPAPADRPDLPPGISSTHLHDQVREGDVL
                     SVRAPAGQFFIDADASIPAVLIAGGIGITPMMSMLRWCLREQPQREVHLYYGLRRSDE
                     HAFKSQLESLAEAHARFHLNVVYSRPDTGDVEGRDYQHAGHVDVDLLRRSLPHGRHRF
                     FVCGPPAMMNSLVPALAAWGVAREDIHHEAFGPAAPRGAPGVPDRPADAAESIEIRFA
                     RTGRTLLWSGQDASLLDFAERNGVAVDSGCRSGSCGSCETKLVSGEVRYADTPDHVIA
                     AGHCLLCVGKPSSPLVLDA"
     gene            complement(6467..7426)
                     /locus_tag="PKV56_09205"
     CDS             complement(6467..7426)
                     /locus_tag="PKV56_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOX68033.1"
                     /translation="MQFSTFSDDPDHNPYPPAERAAAAARSTWVSVGVNLLLTISQIA
                     IGIMSKSQGLIADGIHSLSDLVADFVVLFAGHHSRKDADDDHPYGHQRFETAASLALG
                     GLLMAVGLAMLWSAFHKIQTPEAVATVHSIALWAAGGALIAKELLFRYMLAVAKRVKS
                     SMLVANAWHARSDAASSLVVGVGIAGNLAGYPILDPIAALIVGCMVTKMGWTFGWDAL
                     HDLMDRSIDDQEVAAIRQTLAETQGVSDVHDVRTRKMGDMIVVDAHIEVDAGLTVEQG
                     HDIAVEARRRVMQRHRVLNMMTHVDPWRRPDLDHAIGPGHSAS"
     gene            complement(7560..7724)
                     /locus_tag="PKV56_09210"
     CDS             complement(7560..7724)
                     /locus_tag="PKV56_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68034.1"
                     /translation="MHTSLKKFMRQFTAVVLVSLVPVALVAFLSIPFTLGHHPGEFRE
                     PPVVTLHLEA"
     gene            7864..8271
                     /locus_tag="PKV56_09215"
     CDS             7864..8271
                     /locus_tag="PKV56_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HOX68035.1"
                     /translation="MNTNKLTRNLIAGLSCALGWVAAAQAVTPDAQLAGYSAQAGVAA
                     APERGKAFFTGLHGREWSCSSCHGASPTQQGKHASTGKPIAALAPAFNAERFTDTAKT
                     EKWFRRNCNDVVGRECTPAEKADVLSWLLTLKP"
     gene            8356..8796
                     /locus_tag="PKV56_09220"
     CDS             8356..8796
                     /locus_tag="PKV56_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HOX68036.1"
                     /translation="MVGLAGLPAARAEEGARLAQAPALPKYQQECAACHVAYPPGLLP
                     AASWQRLMSNLPRHFGTDASLDAATEKEISTWLTQNAATRKRMATPPPEDRITRAAWF
                     VRQHDEVPAETWKRASIKSAANCSACHSRADQGDFDEDNVRIPR"
     gene            8768..9502
                     /locus_tag="PKV56_09225"
     CDS             8768..9502
                     /locus_tag="PKV56_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOX68037.1"
                     /translation="MKTTYASPADLPADQGAKPAVLVWDAPVRVFHWLMVLSFAGAYL
                     TAESERWRLLHVTLGYTMVGLVGFRIVWGLIGSRHARFSSFVRGPAAVVRYVRSLFKG
                     QPEHHVGHNPAGALAIVALLGLTLAIGASGWAVYNDVGAEWIEDLHEGAANFMLAVVG
                     VHVAGVLISSWLHRENLVGSMVSGRKPGRPEDGVRNAWRSLAALMLVAVLAFWWLQWQ
                     GAPVADVQAGPATAAAVAGDRDRDDD"
     gene            9529..10200
                     /locus_tag="PKV56_09230"
     CDS             9529..10200
                     /locus_tag="PKV56_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX68038.1"
                     /translation="MRILLAEDDTLLGDGLRAGLRQLGFLVDWVRDGAAAERELRAQP
                     YAAAVLDLGLPLKDGLEVLAGLRRDGITLPVLVLTARDALPERIRGLDLGADDYVIKP
                     VDLHELAARLRALVRRSHGQPQEKLTAQDVALDPAARSVEQSGVPVAVSGREFDLLHA
                     LMLNAGRVLSREQLEQHLYSWGQEVESNAIEVHIHHLRRKLGPALIQTVRGIGYMLQR
                     EAPKA"
     gene            10197..11549
                     /locus_tag="PKV56_09235"
     CDS             10197..11549
                     /locus_tag="PKV56_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOX68039.1"
                     /translation="MTLSARSLQGRLLWLSLVLVVGVWLGTAVSTWFDVRHELDELLD
                     GHLAQAAALLVVQQAGEIEDDDPGVDAPSLHRYAPKVAFQVFHEGRLAMRSANAPVLP
                     MAGPAASFQSGFRTVQIDAVDWRVFAARGAERDVQVYVGEQLDSRESILWAVLRSTLW
                     PMLLALPFLALALGWAVHRGVAPLRRLGLMLSSRQPQLLDPVVVDDAPSEMQPMLAAL
                     NSLFERIGGLMESERRFTADAAHELRTPISAIRMQAQVAQGEADEGLRRHALAATIEG
                     CDRATRLVEQLLTLSRLEAGAAAVGQPVELDAVIRSVASDLAPQAIQKQQTIEVDAAG
                     PARVRGDALLVAVLVRNLIDNAIRYSPSGGAVKVALVVQGRLVRLTIDDSGPGLNESD
                     LQRLGERFFRVVGTGQGGSGLGWSIVRRIASVHLATVEVGRSPALGGLRVMLDWPVDA
                     "
     gene            11651..12325
                     /locus_tag="PKV56_09240"
     CDS             11651..12325
                     /locus_tag="PKV56_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX68040.1"
                     /translation="MRLLIVEDDPSLGDALTTGLRQLGHVVDWFRDGAKADAALVDAP
                     YDAVVLDLGLPGGDGMVWLGRWRGRELTLPVLILTARDGVEDRIAGLDAGADDYLVKP
                     ITIDELAARLRAMVRRSAGRAQAVWQHGDLEYDPAAKIVRWKGQRVELTGFELALLET
                     LLKHPQRVLSKANLQEKLYDWSGTEPESNAIEVHIHHLRRKIDPSIVRTVRGVGYSLG
                     GETAPS"
     gene            12322..13692
                     /locus_tag="PKV56_09245"
     CDS             12322..13692
                     /locus_tag="PKV56_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68041.1"
                     /translation="MSLQRRLLVYLLICAPLVWGVALFVSIRQARQEVNELFDTELIR
                     LARQVQATLSFDVKTTASASPAPARGTPEAGSADMNDLAIAVWDSHGKLLLADREGVQ
                     LTRYPNGSGFRDEELEGKSWRVYYLQSPSGEWLVASGQRAKERDDLVLSLTASQIAPW
                     LLVLPALLAAMAWAVRRAISPVNQLAAELQGRDANDLRRIPDHAAPTELKPLLGAMNG
                     LFRRIEETLDRERRFTADAAHELRTPLAVLRAQWDVVRRSGGEAERAQAEAKLSLGLE
                     RLDRLVTQMLALSRLESPLEAASQLSMATSVQWEPIVEQAVSDCLALAERRHIELACD
                     WPPQGAQPMPMRGDDQLLTVLLRNLLDNAVRYAPEGSLVTLRIAEDHLEIENDGEPLS
                     ADQFSRLGERFHRAAGQQEIGSGLGVSIVRRIAALHGLEPTFSPRADGRGMKVVLAHR
                     AAVSAT"
     gene            complement(13679..14383)
                     /locus_tag="PKV56_09250"
     CDS             complement(13679..14383)
                     /locus_tag="PKV56_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68042.1"
                     /translation="MTISAPASDFHSALALSRRQLMAAFTVAALSACGALLPLSAQAA
                     TVEESVLELQHDWEVVKYQTAGSEREKRYEALADKAHKISEAHAGRAEPLIWEGIIVA
                     SWAGEKGGLGALSLAKKAKAMYEASIAIDASALDGSAYNSLGVLYYKVPGWPIGFGDK
                     DKARDLIQKALAINPKGIDPNYFYGEFLIETKHPEEAVTYLERAQQAPPRPGRQIADV
                     GRREEIRVLLEKIKSR"
     gene            complement(14448..15248)
                     /locus_tag="PKV56_09255"
     CDS             complement(14448..15248)
                     /locus_tag="PKV56_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX68043.1"
                     /translation="MKATEARVVLTGANGGIGQAAAQALVEAGASVMLVGRDEARLTT
                     QAGALTGGMPGAADRVTWEAADLTDPSTLAGLGDSAARWGANVVVHNAGLPSFGRLET
                     LSASDIQQVLNTNLLAPMLLTQALLPHLRRVPQAQVICVGSVLGRLGLPGYSAYCASK
                     FGLRGFAEALRRELSDTSIRVQYLGPRATRTKFNSPGAEDYNRATGTAMDTPQTVATA
                     LVRLLEDGAAERFLGFPESLAVRVNGLAPGLLDGAFDRHRNSLPAATG"
     gene            complement(15245..15955)
                     /locus_tag="PKV56_09260"
     CDS             complement(15245..15955)
                     /locus_tag="PKV56_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HOX68044.1"
                     /translation="MSKKTSFFSRLSEQTAPDRAALLAAPIIQGTLRGEVSLPSYLAF
                     LTEAYHHVKHTVPLLHACQAALPERNTWLRGPLDEYIEEELGHDEWILNDITACGGDA
                     EAVRHGLPGHATEVMVAYAYDTIARGNPLGFFGMVHVLEGTSVSLALMAADRIQKPLA
                     LPDSAFSYLRSHGTLDVEHTAHFELLMDQVEDLQDQAAIVHAARAFFRLYGDVFRGLP
                     MPATASAVASTRAAAVQA"
     gene            complement(16000..17439)
                     /locus_tag="PKV56_09265"
     CDS             complement(16000..17439)
                     /locus_tag="PKV56_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX68045.1"
                     /translation="MITPSDTDVVLDDGLRSWTRAALQAQVDALAGSLTARGTRVFAT
                     LLDNSPAWVAADLAAAQAGIVHVPLPVFFTPQQIGHALRSAGVDTLLTTTELAGHWPQ
                     APVQRIEVAGSALMLVGLPAHPVTMPAGTAKITYTSGTTGAPKGVCLESQAMSRVAQG
                     LVQAMAPLGIRRHLCALPFAVLLENIAGLMAPLASGATCVTLPLQQIGLTGSSSFDAA
                     RFQSVVTDHRPNSVILLPQMLRAWTAHLVQTGRRAPDSLKLVAVGGAAVGARLIEAAR
                     AVGIPAHEGYGLSEGSSVQTLNLPGADRPGTAGRALPHARLRVAADGEIEVAGSLFAG
                     YLGDATPPAEWWPSGDLGEIDSDGFLHLQGRKKHLLITAYGRNVSPEWIETGLRGEPS
                     IAQAVVFGDGQPALGAVLWPVRLNSPDADLQAAVDATNATLPDYARVQRWVRGRAPFD
                     VESGMATANGRPQRAAIWQRHSDALATAD"
     gene            complement(17469..18116)
                     /locus_tag="PKV56_09270"
     CDS             complement(17469..18116)
                     /locus_tag="PKV56_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HOX68046.1"
                     /translation="MLSVMSACDSSSEIDSGGSVSAPPRLLIHPVGDPRRQEVESFIR
                     DIYADRYGADVQQFAPVLASLRDSSGIYAAAGYRSAAPGPLFLERYFDAPIESLLAAH
                     AEPPPARDRIVEVGHLAASRAGEGRNLIRLLGPHLESMNYEWVVSTLTTELRHLFVRL
                     GITPLALGRASLAALGDDAGRWGSYYDHEPVVLAGHLPRAMAQLARRKAGLAGRV"
     regulatory      18340..18455
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18529..19818
                     /locus_tag="PKV56_09275"
     CDS             18529..19818
                     /locus_tag="PKV56_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOX68047.1"
                     /translation="MTITTATVPLNQALTPVDTADRVFRWNEHASLWFSLGVGLLVIQ
                     IGAYLVPAVGTRDAALAIVVGSLIGAGLLAWTARLACDTGLSSAGLMHRTYGSAFARL
                     PVLLNIVQLVGWTAFELVIMREGTLAITQKTFGFELGGVAGIVVPTLLWGAVLTALMA
                     GSMLTLVRRFVSRFGLPLVVASLLWLTWQFWGQVQAQGVETVWMRPGDGSMGLFSAMD
                     LVIAMPVSWLPLVADYARHGRRGGTAFSGTWLGYAVANIWCYSLGVLIASTTEPSGQL
                     AVLLLSVQGGLLAMGLILLDEIDNAYGDVYSGAVSAHSLKPGWSIRRWGLSIAIGCTL
                     LAMLLPMHQLEPFLLMLSSVFVPLYGVILGRLAAGYVVAKPARGVHWLAAGIWIVGIA
                     AYHALATWAPQFGSALPTLALTFVLAWITRPQPEPGS"
     gene            complement(19827..20699)
                     /gene="thiD"
                     /locus_tag="PKV56_09280"
     CDS             complement(19827..20699)
                     /gene="thiD"
                     /locus_tag="PKV56_09280"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOX68048.1"
                     /translation="MNSTDSTALPHPPEARWRYPRVLSIAGSDSGGGAGIQADLKTFS
                     ALGCYGMTAITAITAQNTVGVSAIHGVPVDVLKAQIRAVLDDIGADAIKIGMLHSPEV
                     VGVVAWAIDEYRIKHVVLDPVMVATSGDRLIAQETVQVLVGELFHRVCVVTPNLDEAA
                     LLLGHDIAGIGDLDGAANELLALGTPAVLLKGGHLPGSVVVDVLAMRDGTRQRLQSPR
                     IPSRNVHGTGCTLSSAIAAQLALGLALPQAVAEARRYILAAISAGAQVQTGRGHGPLN
                     HGHAPLPMRLLPID"
     gene            20729..>21462
                     /locus_tag="PKV56_09285"
     CDS             20729..>21462
                     /locus_tag="PKV56_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX68049.1"
                     /translation="MSGARSERAVVLGAGLMGRLLACELAAAGHDVDVYEAGTAEAPG
                     SAAHVAAAMLAPLAESAVTELGVVRMGSHALLRWPELIAQLGRPVFFQQQGTLVLWHR
                     QDAAEATRFQQLLAATARDMPQLPGLEVLDGAGVARIEPSIGERFAQGLYLRGEGQLD
                     NRELLSALLQRLDDLGVRVHWSSPRSPADFDESLGGWLFDCRGLGARSDWSSLRGVRG
                     EVVRLHAPEVTLTRPTRLLHPRYPIY"
BASE COUNT         3268 a         7074 c         7577 g         3543 t
ORIGIN      
        1 gcttgccggc ggcgatgtcg cggaacaggt gcgagatcac gccctgcggc aggttgtagg
       61 cctcgatctc gcctgaaagc gccagggcct gcagcttggg caccagaccc cagtgcccgc
      121 cgacaacgcg cttgaccagt ccggcgtgac cgaagtggtt cagcccgcga tcctggccgt
      181 cgccctggcc ggcggcgtag accagcgtca ggttgcgcgg cgagccgctg ccggtctcgg
      241 cctcgatggc gaggaagcgc tcttccagcg cgatcgcgat gccttctgca aagccgatgc
      301 cgacgaagcc gccggtggcc accgtgtcgc cgtcgtgaat cagtcggacc gcctcggcgg
      361 cgctgacgat cttgttcggc cgggccggtg tgttcgcgcc ggtcttggcg gtattcagat
      421 gcacggaaag tcctccacga tgacgctttt cagtctacga gtggctgcaa cgtgcttcgg
      481 tggcggttat ccctcgatcg atggcgacag gcccccgccg cgttgcgggg aacgccccgt
      541 gcttgctcag cctgcttcgg gtttcgcggc gcggggcgcc gcacgcctga tgtcgcgcag
      601 catgaacagg tagaggcctt ccaccttctc gcgcgcccag ggcgtcttgc gcaggaactt
      661 caggctcgac gcgacgctcg ggtcgctggt gaagcagcgc accggaatgc gttcggccag
      721 gccggcccag ccgtaatgct cgacgagcgc gctgacgatg gcttccagcg tcagtccgtg
      781 cagcgggttt cgcggttgtt ccatgttcag cgagcgggcg tgcgaatttc gagcggtgcc
      841 ggttgggccc ggtccgcctc gaaacgggtt ttggagtgga tgtacacggg ccgctcgccg
      901 atgacaatcg gcgcgacgac atgcaggcac gacgatcggt acagtggcaa cttccgcatg
      961 cggctcttcc gttttttcga tgcgcgctag tgtcgcttcc cctccatttt ccccgagtac
     1021 acaacatgaa tgcacccgta gtcctgatca ccggcgccct ggcgggcatc ggacgtgcca
     1081 ccgcgctggc cttcgccagg gaaggcgcgc gtgtcgtcgt ttccggccgt cacgatgacg
     1141 caggcgccca gctggtggcc gagctgcgcg cgctcggcgc cgaggccgaa ttcgtcagga
     1201 ccgatgtgcg catcgaggaa gaggtgcgca acctcgtcga caagacggtc gcgcgcttcg
     1261 gtcgactcga tgtggcggtg aacaacgccg gcaccgaagg ccagcctggc gtcgttaccg
     1321 agacatctgc cgagaagtac gccggcacct tcgacaccaa cgtgctgggc acgctgctga
     1381 gcatgaagca cgagatgcgc gtgatgctgg cgcagggcag cggcaacatc gtcaacctgt
     1441 cgtcggtgct gggccgcgtc ggcataccag gcgccgcggt ctacgtggcg agcaagcacg
     1501 cggtcgaggg gttgaccaag tgtgccgcgc tcgaaggggc cgcggccggc gtgcgcgtca
     1561 atgcggtgtc gccgggtccg gtcgagaccg gcatgctcga ccgcttcacc ggtggcagcg
     1621 aagccgccaa ggccggcttc atcgcggcca tgccggccag gcgcgcttcg accgctgatg
     1681 aaatcgcgca ggcgatcctg ttcgtggcgt cggacaagtc gagcctgctc aatggccagt
     1741 cgctcgcgat cgacgggggc tactcgagcc agtgatcttc ctggccccgg cggcgtgcac
     1801 ttttcgccct ggtcgagctg accgccgccg ccgagggcct gaagtcccag gcccaggccc
     1861 aggcccagga cccggtgaag gcgatccggg tcttcaacct cgacgaatcg gccacgttcg
     1921 cctgagggcc cggcgcagcc gcgcaggcgt gccggcagcc agcagcacct cgggtcgaat
     1981 ggcacgcgct acctagaatg cgcgaccgcg acacgccgat cgcggacgaa ggaccgaggg
     2041 cacatgaaga agctctttct tgcgctggtc gccgtcgcgg ccctgtatgt cggttggggc
     2101 cagaccgagg gcttgcacca gcgggccggc gtccccgacc tcgagacccg ggcaccggct
     2161 accagccggg ccgacgacat tcgcgctgcc ttctccgaaa gggacagcgg cacccaggtc
     2221 accggcaaag gcgtcgtcac caagctgctg gcggacgact ccgaaggcag ccgccaccag
     2281 cgcttcatcc tgaagctggc ttcgggccag accgtcctgg tcgcccacaa catcgatctg
     2341 gcaccccgca tcgactcgct ggagccagga gattcggtct cgttcaacgg cgtctacgag
     2401 tggaatgcca agggcggggt tgtccactgg acacatcacg acccgagcgg ccgccatgaa
     2461 gccggatggc tcaggcactc aggccagacc taccagtgaa ggccagcgcg cctgcctgcg
     2521 ccatccaggg tgttgacccg gccccgcccc gcgcttcggc tgcgtcgcgc ccgaaaatca
     2581 tccgtctgaa tcgagagcct tttgtcaccc actgaactgc aggaattcct gcatcgaagc
     2641 gtgccgctgt cccgggccat gggcgtggcc gtggaagcga tcacgcccga cagcgtggtg
     2701 ctggccgccc cgttcgagca gaacatcaat cagcacggca ccgtctttgg cggcagttcc
     2761 gcgacgctat cggtgctggc agcctggtcg ctgctgcaca cgcggctgat ggtcgaggga
     2821 atcgacgtga agctcgtcgt gcagcgcagt gccatggagt acgaggcgcc gatggccggt
     2881 cgcttcaccg caacggcttc gcttccaccc ggggcccact ggcggcactt cgtcgatgcg
     2941 attcgagcca ggggcaaggc cagaatccag gtgctggcgg tcgtcggcag ttcgggcccg
     3001 ccggccggcc ggttccaggg tgaattcgtc gccgttcgcg aggccggcag cgcctgaggc
     3061 tgcgataaca ccagatcatt caggaggacc acatgaaacc gaattccctc tcaaaggcga
     3121 tcatcggcag cctgcttttt tccatcggcc tgggcgcggg cttcttcgcg cagaccctga
     3181 ccgactctgc gcagcgtgtc gagcacaagc gcagcgacct ggtcggtgcc ccggacatgg
     3241 aagtgatcgt ctccacggcg gaatacaagc cgggcgagac catcgacctg cacctgcatc
     3301 atggcgccga ggcggtctac gtgatccagg gcgcgaaggt ccaggctccg ggcaaggacg
     3361 cgacccagat tcccaccggc gtctcgttgc tgaaccagcg cgacgtcaag catgcgggct
     3421 tcaccatcgt gggcgatgcg tcgttgaagc tgttcacggt gcatgtcgtg gacaagggca
     3481 agccgctgta cgagtacgcc cggtagggcc ttcccgccga gactttcggt ctgccgtcga
     3541 gcgcgatccc gcatcgatgt cgtgcccttt gacgaactga cgcacctgtc tgagaaacgc
     3601 aggtggcggg atcgccggcg gtgaagttgc cgggtcagtc gcgctcgcgg cgatcgcccg
     3661 atccacccga accttctcgc gtcggctctt cgttggcgct gcgatggcac ttcacgcagt
     3721 tctggaaatc gcggatgcct tcttcctcgt gctccgcgcg caccttggcc ggcgtgtgtt
     3781 catgacagcc gtagcaggtg tatcggctga agtcgctgtt ggtgtggcag gtctcgcacg
     3841 cggtgttgtg gtcgccgtcg aggacgaagt gcttgtcgtg atcaaacgtc gcgggcttcc
     3901 aggccttcgg ggtgtggcac tgcgagcatt cgccggtgat cttccggtgc aggttgtcgg
     3961 tgggcgcctt gtggcaggcc tcgcaggcgg ccagcacggc ttgggacagc aactcgtgct
     4021 tgaaggtggc gggcgtccat cgatcggtgg agtggcagga ggcgcagctg gccttcaggt
     4081 cccggtgcac gttgttcttc ggtgcgctgt ggcatgactc gcagcgatcg cgtgtttcgg
     4141 cacgcagcag ggcgtgcgag aacggcttgc gactgcgctg ggtgagtttg ggtccctggt
     4201 gatcgctgtg gcaggccatg cagtcctgct cgatcagctc ctggtggaac gcgacgtggc
     4261 ccgccgcggg cggaagcggc acgcccttcg tcgtgcgcaa gccgatgtcg gacgtcgcat
     4321 ggcaggcggt gcatcgctcg gaggacgcgc cgcgcagggc tgcatggcag gcgaagcagt
     4381 cggtggccag ttcggcgtgc gcgccgacca gggtgcccgg gctgatcatc aggtgcgggt
     4441 agacgaacac cagcgccacc agggccagca ggttggccac gatcacggcc atcagccagg
     4501 ggcgcttcat ttccagcccc agaacaggaa gatggcgacg atgtgggcca ccgccagaac
     4561 cgcgaacgcg agcgagatcg ggaagtggat gacccgccac tgcttgaccg catcgaaggt
     4621 caggctgtcc cagtagagcc ggtcctcgag cgtgtcgacc gacaggccct gttgctgcat
     4681 cctctgccgc gcctcgtcca gccgctgcct tgaacgcttg agcaggtact tgccgaccag
     4741 tccgctggcg atgttgatgc ccatggccca caccgccagc cagcccagga tcgcattgaa
     4801 atggatcccc gcgtgcacca gcaccagcaa tgaacctgtc caggccatcc gctcatgcag
     4861 gcgcagcagc gttgccggct gtccgctgct gatcagcttg cgcttgcgca tcgagtagcc
     4921 gaacgagccg atgatcagca gcgtgccggg aatcccgagg tagcgcccga tccacaccgc
     4981 gtcgagcagg tggaggatgc cgtcgatcag caatgccgac gcccccagcg cggcgaacgc
     5041 cagcaggaac ggcaccactt cgcggctgat caggcgggcc ttcatcgagg tcatgcgtcg
     5101 agcaccagtg gggaggacgg cttgccgacg caaagcaggc aatgccctgc agcgatgacg
     5161 tggtcggggg tgtcggcgta tctcacctcg ccgctgacga gcttcgtttc gcagctgccg
     5221 caactgccgg agcggcaacc ggaatccacc gccacgccgt tgcgttccgc gaaatcgagc
     5281 agggacgcgt cctggccgga ccagagcaac gtgcgaccgg tccgcgcgaa ccggatctcg
     5341 atcgactccg cggcatccgc ggggcggtcc gggacacccg gtgcgccgcg cggcgcggcc
     5401 ggaccgaagg cttcgtggtg gatgtcctcg cgggccacgc cccaggcggc cagtgcggga
     5461 acaaggctgt tcatcatcgc cggcggaccg cacacgaaga agcgatgcct gccgtgcggc
     5521 aggctgcggc gcagcaggtc gacatcgacg tgccctgcgt gctggtagtc gcggccctcg
     5581 acatcgccgg tgtccgggcg gctgtagacc acgttcagat gaaacctggc gtgggcctcg
     5641 gccagcgatt cgagctgcga cttgaaggcg tgctcgtcgc tgcgacgcag gccgtagtag
     5701 agatgcacct cgcgttgcgg ctgttctcgc aggcaccagc gcagcatgct catcatcggc
     5761 gtgatgccga tgccacccgc gatcagcacc gccgggatgc tggcgtcggc atcgatgaag
     5821 aactgaccgg cgggggccct caccgacagg acgtcgccct cgcgcacctg atcgtggagg
     5881 tgtgtcgacg atatcccggg gggcagatcc ggccggtcgg cgggtgccgg aacccgcttg
     5941 acggtgatgc ggtagtgcga cggatcgggg cagtccgaca gcgagtagca gcgcgtgacg
     6001 gtgcgaacag gcacggcatc gccagcgacc cgcagcgaga acgtcaggaa ctgaccgggc
     6061 ctgaaggcgg gcaggggctg cccgtctgcc ggctccaggt agaaggagca ctgcgactgg
     6121 gcggcatcct cgaactcgcg gcgcgccacg cggaaatcgc gccagcccgg ccaggcgcct
     6181 gccggcgcgg gcgccgcgtt cgcaatcgcc gcctcggggg gcgactccgc cccgcgacgc
     6241 ctgcgccaga gcgccacacc cgctccggcc acgacctgca gcagcagggt ggcgcagatc
     6301 agggccagca gttcaggggc ggtcatgggg ggagggggcc ggcttcaccg gtgggcatcg
     6361 ggttgccatc gggatgcggg gccggaccga gtgtcggcac gtccggtgat cccgccttga
     6421 tcctcgtcag tcgcggggca cggcgtggcg gtccgtcggt gcggattcag gaagcgctgt
     6481 gcccggggcc gatggcgtga tccaggtcgg gccgccgcca ggggtccacg tgggtcatca
     6541 tgttcagcac ccggtggcgc tgcatgacac gccggcgagc ctccaccgcg atgtcgtgcc
     6601 cctgctccac ggtgagcccg gcatcgacct cgatgtgtgc gtcgaccacg atcatgtcgc
     6661 ccatcttgcg ggtgcgcacg tcgtgcacgt cgctgacgcc ctgcgtctcg gccagcgtct
     6721 ggcgtatggc ggcgacctcc tggtcgtcga tcgaccgatc catcaggtcg tgcagcgcat
     6781 cccacccgaa ggtccagccc atcttggtga ccatgcagcc gacgatcaac gccgcgatcg
     6841 ggtcgaggat cgggtagccg gccaggttgc cggcgatgcc gacgccgacc accagcgaag
     6901 aagctgcatc cgagcgggca tgccaggcat tggcgaccag catgctggat ttcacccgct
     6961 tggccacggc cagcatgtac ctgaacagca gctccttcgc gatcagtgcc ccgccggcgg
     7021 cccacagggc gatgctgtgc accgtggcca cggcttcggg cgtctggatc ttgtggaacg
     7081 ccgaccacag catggccagg ccgacggcca tcagcaggcc cccgagtgcc agggaagcgg
     7141 ccgtctcgaa tcgttgatgg ccgtaggggt gatcgtcgtc cgcgtccttg cggctgtggt
     7201 gacccgcgaa cagcacgacg aagtcggcca ccagatcgga cagcgagtgg attccgtcgg
     7261 cgatcagccc ctgtgatttc gacatgatgc cgatcgcgat ctggctgatg gtcagcagca
     7321 ggttgacgcc cacgctgacc caggtactgc gcgcggcggc cgccgcacgc tcggcgggcg
     7381 ggtacgggtt gtggtcggga tcgtctgaaa aggtggagaa ctgcatgggg cccggctcgg
     7441 tgcattggaa cggtggatgg cgtcgggtcg acgcagacat cgtgcctgac tagcgtaccc
     7501 cgcctgggcg acagcgtcct gacagagatc aggtgcggtg tccggcaggt tggcacgcat
     7561 caggcctcga ggtgcagcgt cacgactggc ggctcccgga attcgcccgg atggtgaccc
     7621 agcgtgaacg ggatcgacag gaacgccacc agggcgaccg gaaccagcga cacgagcacc
     7681 acggcggtga actggcgcat gaacttcttc agtgaggtgt gcatggagag ggtgccttgc
     7741 ggcgcgagtg atggaaggca ccaagactgc gtctccagtc ttatgggcgt ctgatcaagc
     7801 tcagcggcgc gcgctaagaa atccctaaga cggctcgccg aacatgccag tcaaggagat
     7861 gccatgaaca ccaacaagct gactcgaaac ctgattgcag ggctgtcctg cgccctgggc
     7921 tgggtcgccg ccgcacaggc cgtcaccccc gacgcgcaac tcgccggcta cagcgcccag
     7981 gccggtgtcg ccgctgcgcc ggagcgcggg aaggcgttct tcaccggtct tcacggtcgc
     8041 gagtggagtt gctcgtcgtg ccacggcgcg agcccgaccc agcagggcaa gcacgcctcg
     8101 acgggcaagc cgatcgcggc gctggccccg gccttcaacg ccgagcgctt caccgacacc
     8161 gccaagaccg agaaatggtt tcgccgcaat tgcaacgacg tcgtgggacg cgaatgcacg
     8221 cctgccgaga aggccgacgt gctgagctgg ctgctgacgc tcaagccctg acccgttcca
     8281 cccgacttgc cacctggaga accttcgatg tctctcgctt tcagattcga tcctcgacgc
     8341 gcgctgctgc tggcgatggt cggcttggca ggcctgccgg ccgcgcgtgc ggaagagggc
     8401 gcgcgcctgg cccaggcgcc tgccttgccg aagtaccagc aggaatgcgc ggcctgccac
     8461 gtcgcctacc cgcccggcct gctgcctgcg gcctcgtggc agcggctgat gagcaacctg
     8521 ccacggcatt tcggcaccga cgcgtcgctc gatgcggcga ccgagaagga aatttccacc
     8581 tggctgaccc agaacgcggc cacccgcaag cgaatggcaa cgccaccgcc cgaggaccgc
     8641 atcacccgcg ccgcgtggtt tgtccgccag cacgacgagg tgccggccga gacctggaag
     8701 cgggcgtcga tcaagagcgc cgccaattgc tcggcttgtc acagccgggc tgatcaagga
     8761 gatttcgatg aagacaacgt acgcatcccc cgctgacctg cccgccgatc agggcgcgaa
     8821 gcccgctgtc ctcgtgtggg acgcgccggt gcgtgtcttc cattggctga tggtgttgag
     8881 ttttgccggt gcctacctca ccgccgagag cgaacgctgg cggctgctgc atgtgacgct
     8941 gggctacacc atggtgggcc tggtcggctt tcgcatcgtc tgggggctga ttggcagccg
     9001 ccatgcgagg ttttccagtt tcgtgcgcgg cccggccgcg gtggtgcgct acgtgcgcag
     9061 cctgttcaag ggacagccag aacaccatgt cgggcacaac ccggccggag ccctggcgat
     9121 cgttgcgctg ctgggactga cgctggccat cggtgcgtcg ggctgggcgg tctacaacga
     9181 cgtgggtgcc gagtggatcg aggacctgca cgaaggcgct gccaacttca tgctcgcggt
     9241 ggtcggcgtg cacgtcgccg gcgtgctgat cagcagttgg ctgcaccggg agaacctggt
     9301 gggctcgatg gtcagcgggc gcaagccggg tcggcccgaa gacggcgtgc gcaacgcgtg
     9361 gcgcagtctg gctgcgttga tgctggtggc cgttctcgcg ttctggtggc tgcagtggca
     9421 gggtgcgccg gtcgccgatg tccaggcagg acccgcgacg gctgcggcgg tggcgggcga
     9481 ccgcgaccgc gacgacgatt gatgccccgg aggcggtagg ctatccgcat gcgcatcctg
     9541 cttgctgaag acgacaccct gctgggtgat ggcctgcgcg ccggcctgcg tcagctgggg
     9601 tttctggtcg actgggttcg cgacggcgcg gctgccgagc gcgagttgcg cgctcagccc
     9661 tacgcggccg cggtgctcga tctcgggctg ccgctgaagg acggactgga agtgctcgcg
     9721 ggcctgaggc gcgatggcat cacgttgccg gtgctggtgc tcaccgcacg ggacgcgctg
     9781 cccgagcgca tccgcgggct cgacctcggc gccgacgact acgtgatcaa gccggtcgac
     9841 ctgcacgagc tggccgcccg gttgcgtgcg ctggtgcgcc gctctcatgg ccagccgcag
     9901 gaaaagctga ctgcgcagga cgtggcgctg gacccggcgg cgcgctcggt cgagcagtcc
     9961 ggagttccgg tggcggtgtc cggccgcgaa ttcgacctgt tgcatgcgct gatgctcaac
    10021 gccggccggg tgctgtcgcg cgaacagctc gaacagcatc tctacagctg gggtcaggag
    10081 gtcgagagca atgcgatcga agtgcacatc caccatttgc gacgcaagct gggcccggcg
    10141 ctgatccaga cggtgcgcgg catcggctac atgctgcagc gcgaggcgcc gaaggcatga
    10201 cgctgtcggc gcgctcgctg cagggtcgtc tgctgtggct gagtctggtg ctggtggtcg
    10261 gtgtctggct gggcacggcc gtcagcacgt ggttcgacgt ccgtcacgaa ctcgacgaac
    10321 tgcttgatgg ccacctcgca caggctgcgg cgctgctggt cgtgcagcag gccggcgaga
    10381 tcgaggatga cgacccgggt gtcgacgcgc cctcgctgca tcgctatgcg cccaaggtgg
    10441 ctttccaggt gttccacgag ggtcggctcg cgatgagatc ggccaacgcc ccggtcctgc
    10501 cgatggccgg ccctgcggcg tctttccagt ccggattccg gaccgtgcag atcgacgccg
    10561 tcgactggcg ggtgtttgcc gcccgcgggg ccgagcgcga cgtgcaggtc tacgtcggtg
    10621 aacagctcga ttcgcgggaa tccatcctgt gggcggtgct gcgcagcacg ctgtggccga
    10681 tgctgctcgc gctgcctttc cttgcgctgg ccctcggctg ggcagtgcac cgcggcgtcg
    10741 cgccactgcg ccggctcggt ctcatgctgt cgtcgcgcca gccgcagctg ctcgatccgg
    10801 tggtcgtcga tgacgcgccg tccgagatgc agccgatgct tgctgcgctc aacagcctgt
    10861 tcgagcgcat cggcgggttg atggaatccg agcggcgctt cactgccgat gccgcgcacg
    10921 agttgcgcac gcccatctcg gcgatccgca tgcaggcgca ggtggcccag ggcgaagccg
    10981 acgaggggct gcgccgacac gcgctcgcag cgacgatcga aggctgcgat cgcgccaccc
    11041 gactggtcga gcaactgctg accctgtcgc gtctggaggc cggcgccgcc gccgtcggcc
    11101 agccggtcga gctcgatgcg gtgatccgca gcgtggcgtc cgatctggcg ccgcaggcga
    11161 tccagaagca gcagacgatc gaggtcgatg ccgccggccc cgcacgtgtg cgcggcgatg
    11221 cgctgctggt ggcggtgctg gttcgcaacc tgatcgacaa cgccatccgc tacagcccct
    11281 ccggtggtgc agtcaaggtg gccctggtcg tgcagggtcg gctggttcgc ctgacgatcg
    11341 acgacagcgg ccccggcttg aacgagagtg acctgcagcg gctgggcgag cgcttcttcc
    11401 gcgtggtcgg aaccggccag ggcggcagcg gactgggctg gtccatcgtg cggcgcatcg
    11461 cgagcgtgca cctggccacc gtggaagtcg gccgctcgcc cgcgctcggt ggcctgcgcg
    11521 tcatgctcga ctggccggtc gatgcgtgag caggaggcgc cgagattcat gaagtccacc
    11581 ggttcgtggc ccgttggtgt ggctgccgat aatgcggaac aaggcccgac cgactcgatg
    11641 gaaccctgac atgcgtctgc tcatcgtcga agacgaccct tcactcggag atgccttgac
    11701 gaccggcctg cgccagcttg gccatgtggt cgactggttc cgagatggag cgaaggccga
    11761 cgccgctctg gtggacgccc cctatgacgc ggtggtgctc gacctgggcc tgcccggcgg
    11821 tgacggcatg gtctggctgg gccgctggcg cggccgcgag ctgacgctgc cggtgctgat
    11881 cctcactgca cgcgacggtg tcgaggatcg catcgccggg ctcgatgccg gcgcggacga
    11941 ctacctcgtc aagccgatca ccatcgacga actcgccgcg cggttgcgtg ccatggtgcg
    12001 ccgctcggcc ggccgcgcgc aggccgtctg gcagcacggg gacctcgaat acgacccggc
    12061 cgcaaagatc gtgcgctgga agggccaacg cgtcgagctc acgggtttcg aactggcgct
    12121 gctcgagacc ttgctgaagc atccgcagcg cgtgctgtcg aaggcgaatc tgcaggaaaa
    12181 gctctacgac tggagcggca cggaacccga gagcaatgcg atcgaggtgc acatccatca
    12241 tctgcgccgc aagatcgatc cgagcatcgt gcgcacggtg cgcggcgtcg gttactcgct
    12301 cggcggcgag acagcgccgt catgagcctt cagcgccggc tcctggtcta tctgctgatc
    12361 tgcgcgccgc tggtgtgggg cgtcgcactc ttcgtgtcca tcaggcaggc aaggcaggag
    12421 gtcaacgagc tcttcgacac cgaactgatc cgtctggcgc ggcaggtcca ggcgacgttg
    12481 agtttcgacg tgaagaccac tgcctcggca tcgccggctc cggcgcgcgg cacgcctgaa
    12541 gctggttccg ccgacatgaa cgacctggcc atcgcggtgt gggactcgca cggcaagctg
    12601 ctgctggccg atcgcgaggg cgtgcagctc acgcgctatc cgaacgggtc gggattccgc
    12661 gatgaggagc tcgagggcaa gtcctggcgg gtctattacc tgcaatcacc gtcgggcgaa
    12721 tggctcgtcg cctcgggcca gagagcgaag gagcgcgacg acctcgtcct gagcctcacc
    12781 gcgagccaga tcgcaccctg gctgctggtg ctgccggcct tgctggcggc gatggcctgg
    12841 gcggtgcggc gcgccatctc gccggtgaat caactggccg ctgaactcca ggggcgcgat
    12901 gcgaatgacc tgagacggat tcccgaccac gccgcaccga ccgaactgaa gcccctgctg
    12961 ggcgcgatga acggcttgtt caggcgcatc gaggaaacgc tggatcgcga gcgccgcttc
    13021 accgccgacg ccgcccacga acttcgcacg ccactggcgg tgttgcgcgc gcagtgggat
    13081 gtggttcggc gctcgggtgg tgaagccgaa cgcgcgcagg ccgaagccaa gctgagcctc
    13141 ggactggagc ggctcgaccg gctggtcacc cagatgctgg cgctgtcccg cctggagtca
    13201 ccgctcgagg cggccagcca gttgtccatg gcgacttcgg ttcaatggga gcccatcgtc
    13261 gaacaggccg tcagcgattg cctggcactg gccgagcgtc gccacatcga actggcctgc
    13321 gactggccgc cgcagggcgc ccagccgatg ccgatgcggg gggatgatca attgctgacc
    13381 gtgctgctgc gcaacctgct cgacaacgcg gtgcgctatg cgccggaggg cagtctggtc
    13441 acgctgcgca tcgcggagga tcacctggag atcgagaacg acggcgaacc gttgtcggcc
    13501 gatcagtttt cccgcctggg cgagcgcttc catcgcgccg cgggccagca ggaaatcggc
    13561 agtggtctgg gcgtgtcgat cgtcaggcgc atcgccgcct tgcacgggct cgaaccgacc
    13621 ttcagcccgc gcgccgacgg ccggggcatg aaggtcgtgc tggcgcaccg tgccgcggtc
    13681 agcgcgactt gatcttctcg agcagcacgc ggatctcctc gcggcgcccg acgtcagcga
    13741 tctggcggcc ggggcgcggc ggcgcctgct gggcgcgttc gagataggtc acggcctctt
    13801 cggggtgctt ggtctcgatc aggaactcgc cgtagaagta gttcgggtcg atgcccttcg
    13861 ggttgatcgc cagcgccttc tggatcagat cgcgtgcctt gtccttgtcg ccgaagccga
    13921 tcggccagcc cggcaccttg tagtacagga cccccaggct gttgtaggcc gatccgtcga
    13981 gcgcgctggc gtcgatcgcg atcgacgcct cgtacatcgc cttcgccttc ttggccaggc
    14041 tgagcgcacc gaggccgccc ttctcgccag cccacgaggc aacgatgatg ccttcccaga
    14101 tcagcggctc tgcgcgcccg gcatgggctt cgctgatctt gtgggccttg tccgcgagcg
    14161 cttcgtagcg cttttcgcgc tccgacccgg cggtctggta cttgacgact tcccagtcgt
    14221 gctgcaactc gagcacggat tcctccacgg tggccgcctg tgcggacagc ggcagcaggg
    14281 cgccgcaggc ggacagggcc gccacggtga acgcggccat cagctgccgg cgcgacaggg
    14341 cgagggcgga gtggaagtcg gaggcaggtg cagagatggt catggttcga tcctggagac
    14401 ggtgatcagg gggagagcgg tgagggcggt cggacagcgg ctgtcggtca tccggtggcg
    14461 gcaggcaggc tgttgcggtg ccggtcgaac gcgccgtcga gcagcccggg cgcgaggccg
    14521 ttgacgcgca cggccagcga ctccgggaag ccgaggaagc gctcggccgc cccgtcctcc
    14581 agcagccgga ccagggcagt ggccacggtc tgcggcgtgt ccatcgccgt tccggtggca
    14641 cggttgtagt cctcggcgcc cggtgagttg aacttcgtcc gggtggctcg cggcccgagg
    14701 tactgaacac gtatcgaggt gtcgctcagt tcgcggcgca gggcctcggc aaagccgcgc
    14761 aagccgaact tgctggcgca gtaggcgctg tagcccggca accccaggcg cccgagcacc
    14821 gaacccacgc agatcacctg ggcctgcggc acgcgccgca ggtgcggcag aagcgcctgc
    14881 gtcagcagca tcggtgcgag caggttggtg ttgagtacct gctggatatc gctcgcggac
    14941 agcgtctcga gtcggccgaa gctcggcagg cccgcgttgt gcaccacgac attggcgccc
    15001 catcgggcgg cgctgtcgcc caggccggcc agggtcgagg ggtcggtcag gtcggcagct
    15061 tcccaggtga cgcgatcggc tgcacccggc atgccgcccg tcagcgcgcc agcctgcgtc
    15121 gtcagccggg cctcgtctcg gccgaccagc atcaccgagg cgccggcctc gaccagagcc
    15181 tgcgcggcgg cctggccgat gccaccgttg gcgccggtga gcaccacgcg agcttcggtg
    15241 gccttcatgc ctgcaccgcc gctgcacgcg tgctggccac cgcgctggcg gtcgccggca
    15301 tcgggaggcc gcggaacaca tcgccgtaga ggcggaagaa cgcacgcgcg gcatgcacga
    15361 tggcggcctg gtcctgcagg tcctcgacct gatccatcag cagttcgaag tgcgcggtgt
    15421 gctcgacgtc cagcgtgccg tgcgaacgca ggtagctgaa ggccgagtcc ggcagcgcga
    15481 gcggcttctg gatccggtcc gcggccatca gtgcaagcga cacgctggtg ccttcaagca
    15541 cgtgcaccat gccgaagaaa cccagtggat tgccgcgtgc gatggtgtcg taggcgtagg
    15601 ccaccatcac ctcggtcgca tgccccggca ggccgtggcg cacggcctcg gcgtcgccac
    15661 cgcaggcggt gatgtcgttg aggatccatt cgtcgtggcc cagttcctct tcgatgtact
    15721 cgtcgagcgg gccgcgcagc caggtgttgc gctcgggaag cgcggcctgg caggcgtgca
    15781 gcagcggcac cgtgtgcttc acgtggtgat aggcctcggt caggaaggcc aggtagctcg
    15841 gcagcgacac ctcgccgcgc agcgtgccct ggatgatcgg tgcggcaagc agggccgcac
    15901 ggtcgggcgc ggtctgttcg ctcagtcggg agaagaagct cgtctttttc gacatcgttg
    15961 ggtgttccgg gtgcggaggg ttctgtgggg tgctgacgct cagtcggccg tcgccaacgc
    16021 atccgagtgg cgctgccaga tggccgcgcg ctgaggccgt ccgttggcgg ttgccatgcc
    16081 tgattcgacg tcgaatggtg ctcgaccgcg cacccagcgc tgcacgcggg cgtagtcggg
    16141 cagcgtggcg ttggtcgcat cgacggcggc ctgcaggtcg gcgtcgggag agttgagtcg
    16201 gaccggccag agcacggcac cgagtgccgg ctggccgtcg ccgaagacca cggcctgggc
    16261 gatgctcggc tcgccgcgca gtccggtttc gatccactcg ggcgacacgt tgcggccgta
    16321 ggcggtgatc agcaggtgct tcttgcgacc ttgcaggtgg aggaatccat cggagtcgat
    16381 ctcgcccagg tcgccactcg gccaccattc ggcgggcggc gtcgcgtcgc cgaggtagcc
    16441 ggcgaacagg ctgccggcga cctcgatctc gccgtcggcg gcgacccgca gacgtgcatg
    16501 cggcagcgcg cgccctgccg tgcccgggcg atcggcaccg ggcaggttga gcgtctgtac
    16561 cgaggagccc tcggacagcc cgtaaccttc gtgtgccgga atacccaccg cgcgcgccgc
    16621 ctcgatcagg cgagctccga cggcagcccc gccaacggcc accagcttga gcgaatcggg
    16681 tgcacgccgg ccggtctgca ccaggtgcgc ggtccaggcg cgcagcatct gcggcagcag
    16741 gatcacgctg ttcgggcggt gatcggtcac caccgactgg aaccgtgccg catcgaagct
    16801 cgacgagccg gtgagcccga tctgctgcag cggcagcgtc acgcaggtgg ctccgctggc
    16861 cagcggagcc atcagcccgg cgatgttttc aagcagcacg gcgaatggca gcgcgcacag
    16921 gtggcggcgg atgcccagcg gcgccatcgc ctggaccagg ccttgcgcca cccggctcat
    16981 cgcctgcgat tcgagacaca cgcccttggg cgcaccggtg gtgcccgagg tgtaggtgat
    17041 cttcgccgtg cccgccggca tcgtgacggg atgcgcgggc agccccacca gcatcagtgc
    17101 gctgccggcc acctcgatgc gctgcaccgg cgcctgcggc cagtgcccgg ccagttcggt
    17161 ggtggtcagc agggtgtcga ccccggcgct gcgcagcgcg tgcccgatct gttgcggcgt
    17221 gaagaacacc ggcaacggca catgcacgat cccagcctgc gcggcggcga ggtcggcggc
    17281 gacccacgcg ggcgagttgt ccagtagcgt cgcgaacacg cgtgttccgc gggccgtcaa
    17341 cgacccggcc agcgcgtcca cctgagcctg cagtgcagcg cgggtccagg atcgcaatcc
    17401 gtcatccagg accacgtccg tgtccgacgg cgtgatcacg gatggatcct ccgctcggcg
    17461 catggcggct agacccgtcc tgccaatccg gccttgcgcc gggccagctg agccatggcg
    17521 cgcggcaggt gaccggccag caccaccggc tcgtggtcgt agtagctgcc ccagcggccc
    17581 gcgtcgtcgc ccagggcagc gaggctggcg cgtcccagag ccagcggcgt gatgcccagg
    17641 cgcacgaaca ggtggcgcaa ctcggtggtc agcgtgctga ccacccactc gtagttcatc
    17701 gattcgaggt gcggacccag cagtcggatc aggttgcggc cctcgccggc ccggctggcg
    17761 gccaggtgcc cgacttcgac gatgcgatcc cgcgccgggg gaggctcggc gtgcgctgcc
    17821 agaagtgact cgatcggtgc atcgaagtag cgctcgagaa acaacgggcc cggagccgca
    17881 ctccggtagc ctgccgcggc gtagatgccg ctgctgtccc gcaggctggc gagcactggt
    17941 gcgaactgct gcacgtcggc gccgtagcga tccgcgtaga tgtccctgat gaacgactcg
    18001 acttcctggc gccgcgggtc gcccaccgga tggatcagca gacggggcgg ggcggatacc
    18061 gaaccacccg aatcgatttc ggagctgctg tcgcaggcgg acatgactga gagcatgtgc
    18121 attccttgag agggcccgct gatgggatgg cacggtgcgc gggcatggtt ggcatcgtct
    18181 cgcaggctca tgaacgaatg cttaagcgat atccggcact tatgaaggat tccttaacga
    18241 gaggcacatc gtcgaaggcc ggcaggtggg ccgttgctga aatgccgaga ccggtgccga
    18301 gcgggggcgg aatctatact cgcgggtgtg ctttcacgag cacaacgctg ggggtgttgc
    18361 gcgcggggtc gatcgatccc gtggggcgtg gctgagaaag accctttgaa cctgattgag
    18421 gtaatcctcg cgcagggaag ctgaccggat cgaggcgtcg ttgccgtgcg cgacggcgtc
    18481 tcagtcgatc cgggtccgac tccctgccgc atgggcacgg agacatcgat gacgatcacc
    18541 actgcaactg ttccactgaa ccaggccctg acgcccgtcg acacggcgga ccgcgtgttc
    18601 cgctggaacg agcacgcatc gctctggttc agcctcggcg tgggactgct ggtgatccag
    18661 atcggcgcct acctggtgcc ggcggtcggc acgcgggacg cggcgctcgc gatcgtcgtc
    18721 ggctcgctga tcggcgccgg cctgctggcc tggaccgccc gtctggcctg cgataccggg
    18781 ctgtccagcg ccggcctgat gcaccgcacc tacggcagcg cgtttgcgcg cctgcccgtg
    18841 ctgctgaaca tcgtgcagct ggtcggctgg accgccttcg agctggtcat catgcgtgaa
    18901 ggcacgctgg cgatcaccca gaagacattc ggcttcgaac tcggcggagt cgccggcatc
    18961 gtggtgccga cgctgctgtg gggcgcggtg ctgacggcgc tgatggcggg ttcgatgctg
    19021 acgctggtgc ggcgcttcgt cagccgcttc ggcctgccgc tggtggtggc ctcgctgctc
    19081 tggctgacgt ggcagttctg gggtcaggtg caggcccagg gtgtcgagac cgtgtggatg
    19141 cgtcccgggg acggcagcat gggcctgttc tcggcgatgg acctggtgat cgcgatgccg
    19201 gtctcgtggc tgccgctggt ggccgattac gccaggcacg ggcgccgcgg cggcaccgcc
    19261 ttcagcggaa cctggctggg ctacgccgtc gccaacatct ggtgctattc gctcggggtg
    19321 ctgattgcca gcacaaccga gccgagcggc cagctggcgg tgctgctgct gtcggtgcag
    19381 ggcgggctgc tggcgatggg gttgatcctt ctcgacgaga tagacaacgc ctacggcgat
    19441 gtctattcgg gcgccgtgtc cgcccacagc ctgaagccgg gatggagcat ccgccgctgg
    19501 ggtctgtcga tcgcgatcgg ctgcacgctg ctggcgatgc tgctgccgat gcatcagctc
    19561 gagccgttcc tgctgatgct gagttcggtg ttcgtgccgc tctacggcgt gatcctcggt
    19621 cgcctggcgg cgggttacgt ggtcgcgaag cccgcccgtg gcgtgcactg gctggccgcc
    19681 ggcatctgga tcgtcgggat cgctgcgtac cacgcgctcg cgacctgggc gccgcagttc
    19741 ggctcggcct tgccgacgct ggcgctgacc ttcgtgctgg cgtggatcac ccggccgcaa
    19801 cccgagcccg ggtcatgacg cgcgcttcaa tcgatcggca gcaggcgcat cggcagcggg
    19861 gcatgaccgt ggttcaacgg gccgtgaccg cggccggtct gcacctgcgc tcctgcggat
    19921 atcgcggcca ggatgtagcg acgcgcctcg gccacggcct gtggcagcgc gagtcccagc
    19981 gccagctgag ctgcgatggc cgacgacagc gtgcatcccg tgccgtgcac gttgcggctc
    20041 ggtatgcgcg gcgactgcag gcgctgacgc gtgccgtccc gcatcgccag cacgtcgacc
    20101 acgacgctgc ccggcaggtg cccgcccttg agcagcacgg caggcgtgcc gagcgcgagc
    20161 agttcgttgg ccgcgccgtc gagatcgccg atgcccgcga tgtcgtggcc cagcagcagc
    20221 gcggcctcgt cgaggttggg cgtgaccacg cagacgcgat ggaacagctc gccgaccagc
    20281 acctgcaccg tctcctgcgc gatcagccga tcgccgctgg tggccaccat caccggatcg
    20341 agcaccacgt gtttgatgcg gtattcgtcg atggcccacg ccaccacgcc gaccacttcg
    20401 ggcgagtgca gcatgccgat cttgatggcg tcagcaccga tgtcgtcgag cacggcgcgg
    20461 atctgggcct tcagcacgtc gaccggcacg ccgtggatcg cgctgacccc gaccgtgttc
    20521 tgcgcggtga tcgcggtgat cgccgtcatg ccgtagcagc cgagcgcgct gaaggtcttc
    20581 aggtcggcct ggatgcctgc gccaccgccg ctgtcggatc cggcgatcga cagcacgcgg
    20641 gggtagcgcc agcgcgcttc gggtggatga ggcagggcgg tggagtctgt ggagttcatg
    20701 ccgaaaatta tgcgttgcgg ggtgggcaat gagcggagca cgttctgaac gcgcggtggt
    20761 gctgggtgcc ggcctgatgg gccggctgct ggcctgcgag ctggcggccg ccggccacga
    20821 cgtcgatgtc tacgaagccg gcaccgcaga ggcgccgggg tcggccgccc acgtcgccgc
    20881 cgcgatgctg gcgccgctgg cggaatcggc ggtgaccgag ctcggcgtcg ttcgcatggg
    20941 atcccacgcg ctgctgcgct ggccggaact gatcgcgcag ctcggtcggc cggtcttctt
    21001 ccagcagcag ggcacgctgg tgctgtggca ccgtcaggat gccgccgagg ccacgcgctt
    21061 ccagcagttg ctggcagcca ccgcgcgcga catgccgcaa ttgcctggcc tcgaagtgct
    21121 cgatggggcg ggcgtggccc gcatcgaacc gtcgatcggt gagcgatttg cccaggggct
    21181 gtatctgcgg ggtgaaggcc agcttgacaa ccgcgaactg ctgtcggcgc tgctgcaacg
    21241 tctcgatgat ctcggcgtgc gtgtccactg gagcagtccg cggtcgccgg ccgatttcga
    21301 cgagtcgctc ggcggctggc tcttcgattg ccgcgggctg ggtgcccgca gcgattggtc
    21361 ttcgctgcgg ggtgtgcgcg gcgaagtggt tcggctgcac gcgcccgagg tcacgctgac
    21421 ccggcccacg cggctgctgc acccccgcta tccgatctac at
//
LOCUS       DAOORV010000056        21373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3008_length_21373_cov_12.667230, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000056 DAOORV010000000
VERSION     DAOORV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3008_length_21373_cov_12.667230"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..156)
                     /locus_tag="PKV56_09290"
     tRNA            complement(78..156)
                     /locus_tag="PKV56_09290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Arg,seq:gcg)
     gene            complement(166..241)
                     /locus_tag="PKV56_09295"
     tRNA            complement(166..241)
                     /locus_tag="PKV56_09295"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Phe,seq:gaa)
     gene            complement(251..326)
                     /locus_tag="PKV56_09300"
     tRNA            complement(251..326)
                     /locus_tag="PKV56_09300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Asp,seq:gtc)
     gene            complement(420..496)
                     /locus_tag="PKV56_09305"
     tRNA            complement(420..496)
                     /locus_tag="PKV56_09305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(459..461),aa:Glu,seq:ttc)
     gene            complement(646..721)
                     /locus_tag="PKV56_09310"
     tRNA            complement(646..721)
                     /locus_tag="PKV56_09310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Gly,seq:gcc)
     gene            1215..1589
                     /locus_tag="PKV56_09315"
     CDS             1215..1589
                     /locus_tag="PKV56_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68050.1"
                     /translation="MSNITLTRRQNALALFQDYAEKALASGAAPKGLEQAFAATLQIS
                     PSMWSQIKSSRPIGDKLARQIESACGKPGGWLDELRKDAAPSATEAAFLELALAAWRS
                     TNSAGRKALREHLKAIVTAPLA"
     gene            complement(1665..3728)
                     /locus_tag="PKV56_09320"
     CDS             complement(1665..3728)
                     /locus_tag="PKV56_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOX68051.1"
                     /translation="MPVLLRYAEAQPRRFMDPAHSPIVAYRRYGYGQSLSTFLATPTE
                     SIIGALTQRSAFNVDQSQVGAWVGTVDALRASLASWGGEGHVFLEFDVPRLGRRIDAV
                     LVLRHVVFVVEFKVGAQAFLAQDLDQVVDYALDLKHFHETSHLVPMAPVLVATAAPAG
                     MVEASLDAAVPHLLHPLRCTPDNLMRAIELALALTDGPLVDPLVWVRGRYRPTPTIVE
                     AAMALYARHSVADISRSDAANLGQTSQFVNDVIARACEQRHKAICFVTGVPGAGKTLV
                     GLDVATQSTNAEDRLHAVYLSGNGPLVQVLQEALARDRVRREAEEGRRLGIGEARRQV
                     KSFIQSVHHFRDDGLADPDPPDDHVAIFDEAQRAWNREQTATFMQRKRGQAGFDLSEP
                     EFLISCLNRHIDWAVVVCLVGSGQEINTGEAGIAEWLGSVERRFPDWKVYLSPRLGEG
                     DPQVAMLKAAVELRGQLIEAPALHLATSVRSFRSERYSEFVNRLLDLDTEGARALLPE
                     ATARFPLRVTRELTKAKAWLRAQARGSERYGIVASSQAQRLRPHAIDVRVKVDPVHWF
                     LSGKEDTRSSYYLEDVATEFQVQGLELDWTCVVWDGDLRYRSEGWSHHSFVGDRWNQI
                     HKPERRSYLLNAYRVLLTRARQGTVIVVPQGDDEDPTRAPAFYDPVFRFLREIGVPVL
                     SDAAN"
     gene            complement(4230..6023)
                     /locus_tag="PKV56_09325"
     CDS             complement(4230..6023)
                     /locus_tag="PKV56_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68052.1"
                     /translation="MNLDDALAALGANRVIWIDDKFNKSSTQLTDLLLGSPEIAKQCG
                     NAAVIDLVEKAAFDETGSRESLQQLIHDMPPTERDKLAQGFFNSETDAKEMAAEELRD
                     SVVESVCKLLSIKLEDRWTFEAALAKVEAVCKANDEKVSYIIDLNQAGGDEKQGLAIL
                     KVLHQYNSKGTAFLLTHSATTSGEAIKEAELQGDLRGNPQQANIPVCVISKARLTDAT
                     DEVDLAAALRIAIKRAGLRRSVHEVLYKLKPDVATAFDEAAKMLLEIPPEQLDEFVVE
                     RGYQEGLSELHVVERAITAHVSQKVREMFARDPGVLQSTARLRSLRAVTLKEGAATPV
                     HEHLDGFRRLEVWESDELINKSFTPLACGDIFQTDEQESKSGTANRLFLLLGQPCDIS
                     LRGEQDRDQEHAMLIPLKKREIKAGASGDDKLKLRPLPFKIDGAHWACDFRSTSAVRL
                     SILDLASLREDGRVRYDAEQPTDVPLLAALHGVIEHRVKAAKEVLAGMSEPSCALQLC
                     FRSTAPFSAIHNGKLVGKETKTIDGKSVKLPQRVTWSLRRVGRIRMPYAAALLRDFLA
                     IQGRDAFDLDFTKEVQAPAPTCAPAAAAISN"
     gene            complement(6036..8624)
                     /locus_tag="PKV56_09330"
     CDS             complement(6036..8624)
                     /locus_tag="PKV56_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX68053.1"
                     /translation="MSDDSASLKVSPHVLVQLGSELVTDVEQALLECVKNSYDADSIG
                     CRIDIQTRESGVITETGTAGRFLNFTEPTDAVQVAIFDAEGKRLDGHPAANTKVDPET
                     SVERQLHYTGKLTIEDNGDGISPGKIASSWLVISGSAKRADGTGPKKTTVKGRTPLGD
                     KGLGRLGTMKLGDILEVESATGADKPLAVARFRWQDCHSAATVDQVPVFTEFRDNVAR
                     FKGTRVNVFGLHDLPDWRRKGRVQELARSFAKLVSPFETTSTFPVSVTLDNHEQSLGS
                     VTDQVLSQAIAKFSFDWGLDAEKKPQLVARAQFMTRLFTTARSRAQREKADLAFNSDG
                     GVGFRTFLDSFSQTKGYEKLQPKSAGSYIEFVRRFPWTDMMLDTGLAIEDPGKFSGAF
                     YFFHLDVRDAPGDEAATGLAVTREIIKDMAGISILRDGFRVRSQGDWLNLSTGMTSGS
                     IYNMRVNNTIGYFALTGAANYRLVEKSDREGFVENAAYRGFFSIATKCRQFANDSLEA
                     VRRGLDAYYKQLNEARRSLPATDPAQVVEESLAASERAQAAARRASDAFTSELEQLEA
                     RVAAGAATGSETARQALNIARKAATAVAAAQASLPQRASLQAAFEQIKVEREAAQEQL
                     LALYESAAVGLSARGLAHELRTHIGDIRQRMTALTRASKNGAVDEQVLSGHVRAMRDS
                     CSAILSAASLIDPMLPRSRMLKETFDVSDFIAEYLEHRRHELLREGVNVLVTPQSAPL
                     VVRMNKSRLLQVVDNLVRNSMYWLHRGHLTKTVARPKEISIEIGNGTLSVADSGPGVD
                     PHYEDSLFEIFVSGKPKDSDGQGLGLFIVKQLLAADGCDIQLSGDRNPEGHRYRFTVD
                     LGTVRT"
     gene            complement(8621..9832)
                     /locus_tag="PKV56_09335"
     CDS             complement(8621..9832)
                     /locus_tag="PKV56_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68054.1"
                     /translation="MLRYPAMMVPKMQGDIIDVVLESAGHDCRVLDPFVGSGTTMTEA
                     VIRGLDFTGIDINPLAALVCEAKAAIDAGADVDDAAQTVIHYLRTDIRESIDVEFHNR
                     SKWFSDESALHLSRLRRGIQEVSDFAARKVLWTAFAETVRKTSNSRTSTYKLHIRKEA
                     DLVEGDRVISIFEDVLRQTLHRVAEYRQGQVSFRKRKPSVQIICSDARTAKIVKSGKP
                     HDILVTSPPYGDNQTTIPYGQFSYLTLQWIPEEDLPNGWSNEHLANTHALDSASLGGS
                     NMDALDKEEAMKLASPAFAAYVQAAMGAGKATSVRKVATFSYDFLEALSHIRSQSRNT
                     AHWIVTTGNRTAAGMRVPFDHICRDVVEFLGGTPIASLRRELPNKRMPSRNSMGEMIT
                     AETTLVAEFTR"
     gene            10717..14331
                     /locus_tag="PKV56_09340"
     CDS             10717..14331
                     /locus_tag="PKV56_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68055.1"
                     /translation="MAIMDYSSGRPYIWDAVAGRARIAQVEDEGALRMPTLTVVELAE
                     WRLSFVEANKGYLQGQALVSAQRWQEQGLPTTYLPFNLQQRWNKELTVRVKQRLQDFF
                     SLWNHGVAIDAEEGTGTSRAEAERAALEAEVAAASDRGDLYSVGALLAQDLSDASPEA
                     AEELLVKVTSAWASTRGVAADIQSLEDLAGRKETFTASNLATAFVNALGRLDDLEAAL
                     PDGVSDFAFRIRDEVRAIYDVERKSPVDMCRAAVATLDHAVAEVRSAIERFVRTTPAT
                     ASTAAVEVLKYAHRLQPLVVPAERQFLRDLDILIGPSFRKLFSAYERSEAVEVLRRAP
                     EFVESMRKQRPSPSDPRQRSQIWTELVKPIFEHLGALIEEATSRGEVAVAPVLALRNP
                     RTKADLSSTNTDIALAFSLVNRGQGHANDISLRQTGKTNGADLVVIEPAGPFDVPPDG
                     EQVVRLRLRLGASSTELDIPIEWSCQSNSGRIVASADCLHVSQQVTEPDWKALLNNPP
                     YSLNPIRQAERLYGRASLLTKLRLAAMAGASHFVWGQKRIGKTSLLQVLAAELSQRDD
                     VTCVLLRMGEVASLHEGQLAHLIAQRLVEKAQAAVQVPAEAEFGASLSRLIPFMEQLM
                     ASKGQHKYLVIIDEFDDLDAAFYTGERGKQFVKSLRSISEVGLTFFFVGSERMEAIYS
                     RHQADLNKWRNIQLDRIDSRTDCRALIAEPVANVIEFSTEAVDFITDFTAGNPFYIHN
                     FCYQVFERCLQEHRTFVDDNDTHAVRDQLLRALGPTNFSHLWEDNPVLELEEKRRDSA
                     ENCVTLACIAVLGGRYEAVEDLAEVQETLPIAVEDRAPAAVLRRACERLTRRGVLSVV
                     GSGTARVISLPIFREWLGGSAVAKLLPQWSDYKEGLRRTQDQAGSGLEALEVPDQSAF
                     PISEDDLLAITPRLVYCGKQKDVAEIRSWLRQFDDDSRIEMAFQLLRRVADKGFINEG
                     AKTLALNVLEQMIQEKRRTTGEGKWQAIKERRDNLALGYFDSEHKSGGSTARELQKIL
                     RPGKLGPAIGLEAWMRAHLEADALLVIGDDFAGTGQTLAEGMAKFQAKIDPAIWKRFV
                     DERRIAVYVMFAFPEAINRMREAFPGIDVVAATLLGDELRACDEGAGIFADEAERRFA
                     EQVLQQIGRELSPRAPMGHGGLGALVVFHNAAPNNSLPILWCSGTVGERPWKALFQRA
                     "
     gene            complement(14847..15845)
                     /locus_tag="PKV56_09345"
     CDS             complement(14847..15845)
                     /locus_tag="PKV56_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HOX68056.1"
                     /translation="MTAAQIVDGVLVQWGERLFYPSNRIVKTPPTPRPDTLTRRKAAV
                     IRGRIVATVVRRAPQVMVKVTGGGRGMSAIAAHFRYISKNGRLQIEDDRELTYRGKES
                     MLDLVEQWRIGGARIPEESHRREAFNIMLSMPHGTNPLAVQQAARAFAKIELVGHRYV
                     MVLHDHQANPHVHLSVRATGMDGSRLNPRKTDLHRWRESFAERLRELGIEAEASRQAT
                     RGEGRRSQELWQLKAKEDGRLSNEAPASKAGDAYAKNRSNALVAWANIFDALRKSDRP
                     EDQDLAERIAGFVKDSTYGRQVQRNRQRDVVPRDRQRSMPEPLPMQRARPDREMDR"
     gene            complement(15842..16420)
                     /locus_tag="PKV56_09350"
     CDS             complement(15842..16420)
                     /locus_tag="PKV56_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68057.1"
                     /translation="MARDGRDRVTVDLRGAGPLIEQRAAAQGLTVAALVRRAVVAMAE
                     SPEAEAAGPAGVDRTEDGALVKVTVRLPSAHALLLASRSRRAEVSQGIYVASLLEGRA
                     LPAASPDRQQAIAALSRSTDQLAMLGMDLNGFMRLLGRVPDDRLEPYRAGVRSLSTRV
                     QQHLDLASQLLIDLQQAASARRAPRVPRARRP"
     gene            complement(16449..17420)
                     /locus_tag="PKV56_09355"
     CDS             complement(16449..17420)
                     /locus_tag="PKV56_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX68058.1"
                     /translation="MRLPWDRSKPSQLDLLATEQPAVHFDASRQAPMAEPEALPQEVP
                     SVPVTESSRQPTGEPLFVPLSALCEDPDNPRTEFPPAELQELAEDIRLHGILQPIVVQ
                     PADAGGLYRIHFGAKRFRAAGLAGLEQVPVTVRPTGADPYAQVAENQKRHGLTPVDLA
                     RFIRGRVDLGESNATIAKRLGMDLTTVAHYLALLDLPAPLAAALKSGRCAAPRTLYEL
                     ARVHADRPQAVVDLLEGDNPITREAVAALRRPARASKPAMKRPAAPKKPLEAPAGVQS
                     VARAERLCTQLAAACSQLRRVGLDQLPAEQLEALRGRLRQLDVLLAP"
     gene            17697..18068
                     /locus_tag="PKV56_09360"
     CDS             17697..18068
                     /locus_tag="PKV56_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX68059.1"
                     /translation="MVAAFPVRFPDQLRQHLRALRKRHGLTQAQLGALVGVSQARIAE
                     IEANPGLVNFEQLMKLLAVLGVSLTLQEEAAVPVATRVPAAKTAKPKATKPRTKPPAR
                     TPEVEPASRRPLVARPKKGSW"
     gene            18089..19384
                     /locus_tag="PKV56_09365"
     CDS             18089..19384
                     /locus_tag="PKV56_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOX68060.1"
                     /translation="MNGELVGTWRVDRNSHAFRYDPSWLQSPRRRSLSLSLLISGSLE
                     IQGQQVRNYFDNLLPDNERIRSRLRRRFGLRSSEIFELLEAIGRDCVGAVQLLPEGVT
                     PEGWDRIDCDPLTEAQIVDLLQAVPSETDPGSIHDDDLFRISIAGAQEKTALTRWKGR
                     WCRPHGATPTTHIIKLPLGLIGGSKRVDASDSVQNEWLCAQIIAELGLPVASTSLETF
                     GDQTVLIVERFDREWMDKGKWIARLPQEDFCQALGVGPDQKYEQGGGPGMQKCLQLLQ
                     GSADREDTTLFLLTQLAFALMAATDGHAKNFSIYLDRGDAYRMTPLYDVLSMWPYYGD
                     GANQFRPRKAGLAMAVRSKNAHYLFHTIQARHWQQLAARHGGNAVWAAMVELVSSVED
                     ALARVERRLPKDFPAHTWESISTGMRSEAKRFLREAGLL"
     gene            complement(19381..19596)
                     /locus_tag="PKV56_09370"
     CDS             complement(19381..19596)
                     /locus_tag="PKV56_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HOX68061.1"
                     /translation="MQHVHLATETVRPAILRLPEVMRLTGLGRSTIYRLLAAGQFPSP
                     VQLSVRAVGWRRSDVDNWTAERPQVSH"
     gene            complement(19682..20971)
                     /locus_tag="PKV56_09375"
     CDS             complement(19682..20971)
                     /locus_tag="PKV56_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOX68062.1"
                     /translation="MARHLIPSDATLKAVRPGDPRKRLNDGDGLYLLLFVKGGAHAWR
                     FDYSYHGRRKTISLGTYPDTSLALARRKADEARQLVAEDIDPSQKRKAEKESWAQERI
                     ADEREAQGLPPIDSFEAVAREWYAVKKDGWSPSYGERIIARLENDVFPYIGKVPVADV
                     TPPLLLEVMRRIEARGVLETAHRALQDCSQALRYAVATGKAPSNPARDLKGALRRPMA
                     KHFPAITEPKRLGELLRAIDSYAATPVVRAALKLAPMVLLRPGELRFAEWPEIDLDAA
                     MWTVPAIRMKRELRQKLHGAPHLVPLPRQAVEVLRELHPLTGHAKMVFRGERHHDRAM
                     SENTINAALRAMGFSADEVTGHGFRATARTMLQENLGFDPDVIEAQLAHAVRDPLGRA
                     YNRTEFLEQRRKMLQAWANYLDKLRQGAPVVRLSPKA"
     gene            complement(21155..21230)
                     /locus_tag="PKV56_09380"
     tRNA            complement(21155..21230)
                     /locus_tag="PKV56_09380"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21195..21197),aa:Phe,seq:gaa)
     gene            complement(21273..>21373)
                     /locus_tag="PKV56_09385"
     CDS             complement(21273..>21373)
                     /locus_tag="PKV56_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX68063.1"
                     /translation="VAEKTGLERTHLYRKLKQLGVDLSRNKRGGVL"
BASE COUNT         3850 a         6770 c         6744 g         4009 t
ORIGIN      
        1 ccccgacgtg gcttcgaacc acggacctcc tggttaacag ccaggcgctc tgccaactgc
       61 gctatcgggg atctggatgg cacctcgtgc acgacttgaa cgtgcgacct ccggcttcgc
      121 agatcggcgc tctgaatcca actgagctaa cgaggttctg gttgctggtg ccgagagagg
      181 gcttcgatcc cccgacgcac gcgccttcaa cgcgccgctc tacctgctga gctatctcgg
      241 caaactgatc tggcgcctcg tgcgggagtc gaacccgcgg cttccggcta gacaggccgg
      301 cactctggcc gctgagttaa cgaagcaaac attccatccg gtgacgggtt ccggcgccgc
      361 ccaatcgttg cggccggttt ccgccctcga agcgtgagct cgacgagggc ttcttgaatt
      421 ggtgtcgcgt gcgggattcg aacccgcgtt cccggaatga aagcccggtg tcctcggccg
      481 cctagacgaa cacgacgaaa ctgaaagtcc acttcggctg tgcccaaacg cgagcatccc
      541 gtcggatgtg gtgtctcgct gcacggggcc tggccaaggg tcgcgcacat cccggaccac
      601 caacaccatg tcgcgtggcc tcggagtgca tggacacacc cgaagtggag cggatgacga
      661 gtctcgaact cgtggcctcg gccatggcgt ggccgcgctc tgccaactga gctacatccg
      721 caaaggtgca agacggacct tcggccagta ccgaggtcgt cttgcggcaa cttgttaaag
      781 agcatccacc gtcgccggtg gtggcgatcc atcgatcgcc aggcccgcgc cagcgcgcaa
      841 gccttgtgaa gagacaaaga aaaaggcccg gaacctttcg gattccgggc ctctgcgaac
      901 agagcttgga aggcgcgcgg ctaggcgctc ccttctcccg gattcaacgg accctcgctc
      961 cgatcaaggc aatagcccgt cggattggcc ccgtgcatcg ccaccgctgc gaacaacggg
     1021 acgaacgcga ccagcccctg caggggtcgg tgggtccgaa tgacgttgtg caatttcacg
     1081 gtgatgtcct gtagttctgg ctgtggctcc ggagagccgg tttctcgatg ggagcggact
     1141 gtaaagactc tttacccgac tcgtcaaccc cctgcgagaa tggcgtccga cttcgttgca
     1201 tcgcgtacac acccgtgtcg aacatcaccc tcacgagacg acagaacgcg ctggccctgt
     1261 tccaggacta cgctgagaag gcactggctt cgggcgcagc gcccaagggg ctcgaacagg
     1321 ccttcgcggc aacgctgcag atctcgccga gcatgtggag ccagatcaag tcttcgcgcc
     1381 cgattggcga caagctggcg cggcagatcg agtcggcttg tggcaagccc ggaggctggc
     1441 tcgacgagct tcgcaaggat gccgcgccgt cggccaccga ggcggcgttc ctcgaactgg
     1501 cgcttgccgc ctggcgctcg acgaacagcg cgggtcgaaa ggcactacgt gagcacctca
     1561 aggcgatcgt cacggcaccg ttagcctgac gctgcgctcg attccgtagc gaagcactgt
     1621 tgtgtcacgg aagacacaag acgagcggca gcggccttcg gcgctcagtt cgcagcatcg
     1681 ctaagcaccg gtacgccgat ctcgcgaagg aaccggaaga ccggatcgta gaacgctgga
     1741 gcccgcgtcg gatcctcgtc atctccctgc ggcacaacga tcactgtgcc ttggcgagcg
     1801 cgcgtcagca agacgcggta tgcgttgagt aggtaggacc gcctctctgg cttgtggatc
     1861 tgattccaac gatcgccaac aaagctgtgg tggctccagc cctctgagcg atagcgcagg
     1921 tcgccgtccc agacgacaca ggtccagtcc agttcaaggc cctgcacctg gaactcagtc
     1981 gcgacgtctt cgagatagta ggaggagcgt gtgtcctcct tgccggagag gaaccagtgc
     2041 acggggtcca ccttgacccg cacgtcaatc gcgtgtggtc gcaggcgctg tgcctgggac
     2101 gaggcgacga tgccatagcg ctcgctgccg cgggcctggg cccgcaacca ggccttcgcc
     2161 ttggtgagtt cgcgcgtgac ccgcagcgga aagcgggcag tggcctcagg caggagtgcg
     2221 cgggcgccct ctgtatcaag gtcgagcagg cggttcacga actccgagta gcgctcggat
     2281 cggaatgaac gcactgaggt cgccaggtgc aatgcgggag cctcgatgag ctggccacgc
     2341 agttcaaccg ctgccttgag catggcgacc tgcggatcac cttcgcccag ccggggcgac
     2401 aggtagacct tccagtcggg gaagcggcgc tcaaccgagc ccagccattc ggcaatacca
     2461 gcctcgccgg tgttgatctc ctgtccgctt ccgacaaggc acaccacaac ggcccagtcg
     2521 atgtggcgat tgagacaaga gatcaggaac tctggctcgg aaaggtcaaa gcccgcctgg
     2581 ccgcgcttgc gctgcatgaa ggtcgcggtc tgctcgcggt tccacgcgcg ctgggcctcg
     2641 tcgaagatgg cgacgtgatc gtctggcggg tccgggtcgg cgagaccatc gtcgcggaag
     2701 tgatgcacgc tctgaatgaa gctcttgacc tgccgccgcg cctcgccaat gccaagtcgc
     2761 cggccctctt cagcctctcg ccttacgcgg tcccgcgcca gtgcttcctg cagtacctgc
     2821 accagcgggc cgttgcccga cagatagacg gcgtgcaacc ggtcctcagc gttggtgctc
     2881 tgcgttgcaa cgtcgagccc caccaaggtc ttgccggcac cggggactcc ggtcacgaag
     2941 caaatcgcct tgtggcgctg ctcgcacgcg cgggcgatca cgtcattgac gaactgcgag
     3001 gtctggccca gattcgcggc gtcgctgcgg gagatgtcag caacggagtg gcgcgcgtag
     3061 agcgccatcg cggcttcaac gatggtcggc gtcgggcggt agcgcccgcg cacccagacc
     3121 agcgggtcga ccagcggccc gtcggtaagt gcgagcgcca gctcgatggc ccgcatcaag
     3181 ttgtcgggtg tgcagcgcag tgggtgcaga aggtgaggca cagcggcgtc cagagacgct
     3241 tcaaccatgc ccgccggtgc ggccgtggca accagcacgg gcgccatcgg gaccagatgg
     3301 ctcgtctcgt ggaagtgctt gaggtccagc gcgtagtcga ccacttggtc aaggtcctgc
     3361 gcgaggaacg cttgcgcacc gaccttgaat tcgacgacga ataccacgtg tcgcagtacc
     3421 agcactgcgt caatgcggcg gccgaggcgc ggcacgtcga actccaggaa gacgtggcct
     3481 tcgccgcccc aggaagccag cgaggcgcgg agcgcgtcta ccgtacccac ccatgccccg
     3541 acctggctct gatcgacgtt gaaggcagat cgctgagtga gcgcaccgat gatcgattcg
     3601 gtcggcgtcg cgaggaatgt cgaaagcgac tgcccgtagc cgtagcggcg ataggcaacg
     3661 atagggctat gggctggatc catgaagcgt ctgggttgtg cctcagcata gcgaagcaga
     3721 accggcacat ccagtgatgc gctcggcgag ggtggctgcc gtcgcgctgg gcgcgccctg
     3781 gcagccaatt gctggcggcc agcctatcct gcactcaaac tacagatcga tggtcttgca
     3841 cgcttgcgaa tcttcagcga ccactacaga gcattggtcg gacagtccga cccctcgggc
     3901 ctcttgtaga tttgaccctg ccggacggca aaaggtgact cattggccgc cgttactcag
     3961 gctgtgagcg accgagctat agcgatagcg atagcggtct tgcgtaccag attcaaccag
     4021 tgcctgcgtt gcgcgcaagg cccagccgct gactttgtga cgcgcacact agtgcttcta
     4081 ggttggcgcc tgagcgcaat cggcctcaaa cggctgcgtc cacagtcgga tcgccctaac
     4141 gcatggaccc ctgccattgg caagcaaccc cctgtctcct tgtcgcctga tctaccgcag
     4201 agcaagcacc acgtacttct ggatgcggcc tagttgctta tcgctgcagc ggctggagca
     4261 caggtaggcg caggtgcctg cacctccttt gtgaagtcga ggtcgaaggc gtcacgacct
     4321 tggattgcaa gaaagtcgcg cagcagcgcc gcggcgtagg gcattcggat gcgcccgacg
     4381 cggcgcagtg accatgtcac gcgctgaggc agcttgactg acttgccatc gatcgtcttg
     4441 gtctctttgc ctaccagctt gccgttatgg atggcactga atggcgcagt cgagcggaag
     4501 cacagctgga gagcgcatga cggttcgctc attcctgcca gcacttcctt cgcggccttg
     4561 acacggtgct cgatgacgcc atgcagcgcg gccagcagcg gcacatcggt cggctgttcc
     4621 gcgtcgtatc gcacgcgccc gtcctcgcgc aaggacgcca agtccaaaat gctcaggcgc
     4681 accgcgctgg tagagcggaa atcacaggcc caatgcgcgc cgtcaatctt gaacggcagt
     4741 ggccgaagct tgagtttgtc gtcgcccgac gcgcctgcct tgatctcgcg cttcttgagt
     4801 ggaatgagca tcgcgtgctc ttggtcgcgg tcctgctcac ctcggagcga gatgtcgcat
     4861 ggttgcccga gaagtaggaa gagcctattg gcggtgccgg acttcgactc ctgctcgtcg
     4921 gtttggaaga tgtctccgca tgccaatggc gtgaacgact tgttgatgag ctcgtccgac
     4981 tcccaaacct ccaggcgccg gaagccatcg aggtgctcgt gcaccggtgt cgcagcgcct
     5041 tccttcagcg tgacggctcg cagtgagcga aggcgggcgg tcgactgcag gacccctgga
     5101 tcgcgggcga acatttcccg tactttctgg gagacgtgcg cggtgatcgc acgctcaaca
     5161 acgtgcagtt ccgagaggcc ttcttggtaa ccccgttcaa cgacgaattc gtcgagttgc
     5221 tcgggaggaa tctccagcaa catcttagcg gcctcgtcaa aagccgtcgc cacatctggc
     5281 ttaagcttgt ataggacctc gtgaacactt ctgcgcaaac ctgctcgctt gatcgcaatc
     5341 cgcagcgccg ctgccaagtc gacttcgtca gtggcatcag tcaggcgagc tttcgagatc
     5401 acacacaccg gaatgttggc ttgttgcgga ttgccacgca ggtcgccttg aagttccgct
     5461 tccttgatcg cctcgccgct ggtcgttgcg gaatgggtta gcaagaaggc cgtgcccttg
     5521 gagttgtact gatgcagcac cttcaggatc gcgaggcctt gcttctcatc gccgccggct
     5581 tggttgagat cgatgatgta gctgaccttc tcgtcattcg ccttgcagac ggcctcgacc
     5641 ttggcgagcg ccgcctcgaa ggtccagcga tcttccagtt tgatggatag cagtttgcag
     5701 acgctctcga ccacgctgtc tcgcaactcc tcggctgcca tttccttggc gtctgtttcg
     5761 ctattgaaga agccctgggc aagtttgtcc ctctcggtgg ggggcatgtc gtggatcagt
     5821 tgctggagcg actctctgct gcctgtttca tcaaacgcgg ccttctcaac caaatcgatg
     5881 acagcggcgt tgccgcactg ctttgcgatt tccgggctgc ctaacaacag gtcggtcagt
     5941 tgcgtcgagc tcttgttgaa cttgtcgtcg atccagatca cccgattggc acccagtgcc
     6001 gccaaagcgt cgtcgaggtt catatgtctc cctgcctatg tgcgcactgt gccgagatcg
     6061 accgtgaatc gataccggtg tccttctggg tttcgatcgc cactgagctg aatgtcacaa
     6121 ccgtcggcgg ctaggagctg cttcacgatg aagagcccca ggccctgccc gtcactgtcc
     6181 ttgggctttc ccgacacaaa gatctcgaag agcgagtctt cgtagtgggg atcgacgcca
     6241 ggccccgagt cggccactga caaggtcccg ttcccgatct caatcgaaat ctctttcggt
     6301 cgagccaccg ttttcgtgag gtgaccgcgg tgcagccagt acatgctgtt gcgaaccagg
     6361 ttgtccacta cttgaagcaa ccgggacttg ttcatgcgca cgacaagcgg cgcgctctgt
     6421 ggagtcacga ggacgttcac cccttctcgt agcaactcgt gacgacgatg ctcgaggtac
     6481 tccgcgatga aatcgctcac atcgaaggtc tctttgagca tacgagacct tggcagcatc
     6541 ggatcgatga gcgaggcggc gctgaggatg gctgagcagg agtcacgcat ggcgcgcacg
     6601 tgccccgaca gaacctgctc gtcgactgcg ccgttcttcg acgcccgcgt caacgccgtc
     6661 atgcgctgac ggatgtcgcc gatgtgtgtg cgcaactcgt gagccaggcc gcgggcgctc
     6721 aagccgaccg cggcactttc gtacagcgcg agcagctgtt cctgtgctgc ttctcgctca
     6781 accttgatct gctcgaacgc agcctgaagg ctcgcgcgct gcggaagact ggcctgcgcg
     6841 gccgccaccg cggtggcggc cttgcgggcg atgttgagtg cttggcgtgc cgtctcagat
     6901 ccggtagccg cgccggcggc gactcgcgcc tcaagttgct ccaattcaga cgtgaacgcg
     6961 tcactggctc gcctagcggc agcttgcgcc ctttccgaag ctgccagcga ctcttcgacg
     7021 acctgggcag ggtcggtggc cggcagcgac cggcgcgctt cgtttagctg tttgtagtac
     7081 gcatccaggc cacgacggac cgcctccaac gagtcgttcg cgaactggcg gcatttagtc
     7141 gcgatggaga agaagccgcg gtaagccgcg ttctcaacga agccttcgcg gtcggacttc
     7201 tccacgagcc gatagttggc ggccccggtc agggcgaagt agccgatcgt gttgttgact
     7261 cgcatgttgt agatgctgcc cgatgtcatt ccggtggaca gattcaacca gtcgccctgc
     7321 gagcggacgc ggaaaccatc tcgaaggatg gaaatgccgg ccatgtcctt gatgatctct
     7381 cgagtgactg ccaaccccgt cgctgcctca tcgccggggg cgtcgcgtac gtcgaggtga
     7441 aagaagtaga acgcgccgct gaacttgcct ggatcttcga tcgcgagacc tgtatctagc
     7501 atcatgtccg tccaagggaa tcgtcgaacg aactcgatgt agctaccggc cgacttcggt
     7561 tgaagcttct cgtagccttt cgtctggctg aaggagtcga ggaacgtcct gaaaccgacg
     7621 cctccgtcgc tgttgaaggc cagatccgcc ttttcacgtt gtgccctgct gcgggcggta
     7681 gtgaagagcc gggtcataaa ttgagcccgc gccaccaact gtggcttctt ctctgcgtcg
     7741 agcccccaat cgaagctgaa tttcgcgatg gcctggctca gcacctggtc ggtgacagac
     7801 cccaaggact gttcatgatt gtcgagcgtg acactcacgg ggaaagtcga ggtcgtctcg
     7861 aacggagata cgagcttggc gaagctcctt gcgagctcct gcacccgccc cttacggcgc
     7921 caatcaggca ggtcatgcaa tccaaatacg tttacccgcg tgcctttgaa gcgcgctacg
     7981 ttgtctctga actcggtaaa cacgggcact tggtccactg tcgcggcgct gtggcagtcc
     8041 tgccatcgga accgcgccac agcaagcggc ttgtccgcgc cagtggcgga ttcgacctcc
     8101 agaatgtcgc ccagcttcat cgtgcccagg cgtcccagcc ccttgtcccc cagcggcgtg
     8161 cggcccttga cagtcgtctt cttgggcccg gtgccatcgg ctcgcttagc cgacccactg
     8221 atcaccagcc atgacgacgc aatcttgcct gggctgatgc cgtcaccgtt gtcttcaatg
     8281 gtcaacttgc ctgtgtagtg aagctgacgc tcgacggatg tctccgggtc gactttggtg
     8341 ttcgctgcgg ggtggccatc gagccgtttg ccctccgcgt cgaagatcgc cacctgcact
     8401 gcgtcggtcg gctcggtgaa gttcagaaac cttcccgcag tcccggtctc ggttatcacg
     8461 cccgactctc tggtctggat atcgatgcgg caacctatcg aatcggcgtc gtacgagttc
     8521 ttgacacact ccagcagggc ttgctcgacg tcggtgacga gctcgctacc gagctggacg
     8581 aggacatggg ggctgacctt cagcgacgca ctgtcgtcgc tcatcgggtg aactcggcaa
     8641 ccagggtagt ctctgctgtg atcatctcac ccatcgaatt tctggaaggc atgcgcttgt
     8701 tcggcaactc gcgccgcagg gatgcaatcg gagtgccgcc gaggaactcg acgacgtcac
     8761 ggcagatgtg gtcaaacggc acacgcatgc cggcagcggt gcggttgcct gtggtgacaa
     8821 tccagtgcgc ggtgttgcgc gactgggagc gaatatggct cagcgcctca aggaagtcgt
     8881 agctgaaggt cgcgaccttc cggacagacg tggccttgcc agcgcccatc gctgcttgca
     8941 cgtatgcggc gaatgccggt gacgccagct tcatggcctc ctctttgtca agcgcgtcca
     9001 tattcgaccc accgagactg gcactgtcca gcgcgtgcgt gttggccagg tgttcatttg
     9061 accaaccatt cggcagatcc tcctctggaa tccactgaag cgtcaggtac gagaactggc
     9121 cgtagggaat ggtcgtctga ttgtcaccat aaggaggcga ggtcacaagg atgtcgtgag
     9181 gcttgccgct cttgacgatc ttggctgtac gcgcatcgct gcagatgatc tgaacgctcg
     9241 gcttgcgctt gcgaaagctg acttgaccct gccgatactc cgcgacacga tgcagcgtct
     9301 ggcgcagcac atcctcgaag atcgagatga cacggtcacc ttctactagg tccgcctcct
     9361 tgcgaatgtg caacttgtag gtgctggttc tggagttcga agtctttcgg accgtttcag
     9421 caaatgcggt ccaaagcact tttcgagcgg cgaagtccga cacctcttga atgccacgcc
     9481 gcagtcgaga caggtgcagc gcgctttcat cgctgaacca cttcgagcga ttgtggaact
     9541 cgacatcaat actttcacgg atgtcggtcc gcaggtagtg aatgacagtt tgcgctgcat
     9601 cgtcaacgtc cgcgcccgca tcgatcgcgg ccttggcctc gcaaacgagc gcagccagcg
     9661 ggttgatatc gatgcccgtg aagtccaagc ctcgaatgac cgcctcggtc atcgtggtac
     9721 cagagccaac gaagggatcg agcacgcggc agtcgtggcc agcagactct aggaccacgt
     9781 cgatgatgtc gccctgcatc ttcggaacca tcatcgccgg ataacgcagc aagccatgaa
     9841 ggtttggccg agcattcagc ccgacctcgc gccatgccac aggcgggttc gaggcgagcc
     9901 tctgcaactg gctagcgagg cggcggcgag gaagggaggt cggtggctgg gccattttgc
     9961 tggccctcga gtcatgttga ggcgccaggc gaatcttaag atcaccaggg caggccccgc
    10021 tacatgtttc tgtgagggcg gcgcaggagg cagactcgcc tcacagcgga cgtggcgggc
    10081 ctccaacgcc ctggggcgag ccagtggcga gggcccttcc cttaccgcct tcccggcaac
    10141 gcgggagcag tgcttggtgt tcgatgaagt ttcccgtcac ctccttgcag cttagcctgg
    10201 tttgttggcg gcccagaagc ggtgatcacc ccggctccca ccgtccacct agaaacccgt
    10261 gtgtgccgta gcgcagttcg gcactgtgct gcgccagaat gactggcaac gcctaggagg
    10321 gagccgctga tgagaacaag agaacaggcc ctcgagctcg tcgaggccgc cttcttgcag
    10381 gctcgacgca agacggggtc tgaggcccgc cagatgactc tggctgtgct gaacaatcgc
    10441 ctcctgcaga tgacggacca taggtttcga ccacaggact tcggcgcgaa ggacctgcgg
    10501 gcgttcgcct cactgctcca ccctgatctg cgcatcgtag gcgagccgaa tcgcccggca
    10561 gtcgagtggg cttcggggag tgactccgcg gaggcgggcc ctgctcgacc gtcggctgca
    10621 cccaataact catctgacac tgggcctgtt caatcgacgg taggtccctc cgtaccgctg
    10681 cagggggaag ccggtcgtgt gcgcaaggac ttgtggatgg ccatcatgga ctacagcagc
    10741 ggccgtccct acatctggga cgcagtcgca ggacgagctc gtatagccca ggtcgaggat
    10801 gaaggcgcct tgcgtatgcc gacgctgacg gtggtcgagc tggcggaatg gcggcttagc
    10861 tttgtcgaag cgaacaaggg gtatctgcaa ggacaagcgc tggtgagcgc gcagcgatgg
    10921 caggagcaag gcctgccaac cacctatctg ccgttcaacc tgcagcagcg ctggaataag
    10981 gaactaacag tacgcgtgaa gcagcgattg caagacttct tctctctatg gaatcatggc
    11041 gtcgcgatcg atgccgagga aggcacagga acctcccgcg cggaagcgga gcgtgcggca
    11101 ctggaagccg aagtggcagc ggctagcgat cgaggtgacc tctactcggt cggtgccttg
    11161 ctggctcagg acctgtccga cgcgagtccg gaagcagcag aagaacttct tgtcaaagtg
    11221 acaagcgctt gggcgagcac gcgcggcgtg gccgccgata tccagtccct ggaggacctt
    11281 gctggccgca aggagacgtt caccgcctca aatctggcga ccgcctttgt gaacgcgctg
    11341 ggccggcttg acgatcttga agctgccttg ccggacggag tcagcgactt tgcgtttcgg
    11401 attcgggatg aggtccgagc aatctacgac gttgagcgga agtcgcccgt tgacatgtgc
    11461 cgtgcagctg tcgccacctt ggatcacgcg gtggccgagg tcagatcggc aattgagcgc
    11521 ttcgtgcgca caaccccggc aacggctagc accgcggctg ttgaagtcct gaagtacgcg
    11581 cacagattgc aaccgctggt tgtgccggcc gagaggcagt ttcttcgtga cctcgacatc
    11641 ctaatcggcc cgtcgttcag gaagctcttc agtgcctacg agcgtagcga agccgtcgaa
    11701 gtccttcgga gagctcccga gtttgtcgag agcatgcgca agcagcgacc aagcccgtct
    11761 gatccgcgac agcggagtca gatttggaca gaactggtga agccaatctt cgagcacctt
    11821 ggggcgctga tcgaagaggc cacgtcccgt ggtgaagttg cggtggcacc cgtcctggcg
    11881 ctccgaaatc caaggaccaa ggccgacctg agctcgacga acacagacat cgctttggcg
    11941 ttctccttgg tcaaccgagg ccaagggcac gcgaatgaca tctcgttaag gcagacaggc
    12001 aagaccaacg gcgccgacct tgtagtgatc gaaccggcag gccctttcga tgtccctcca
    12061 gacggcgaac aagtcgtgcg tctacgcctg cgcttgggcg cttcaagcac agagctggac
    12121 atccctatcg agtggtcgtg tcagtccaac tcgggaagga tcgtggcaag cgccgactgc
    12181 ctgcatgtct cacagcaggt gactgaaccc gactggaagg ccctgctcaa caacccacca
    12241 tacagcctca atccaatacg gcaggccgag cgactgtacg ggcgcgcatc attgctgacc
    12301 aagctgcggc ttgctgccat ggccggtgca tcgcacttcg tctggggtca gaagcgcatc
    12361 gggaagactt cgttactcca ggtcctggcc gccgagctct ctcaaagaga cgacgtcact
    12421 tgcgttctcc tgcgcatggg cgaggtcgcg tcgcttcacg aggggcaact tgcgcatttg
    12481 atagcacaac gcctggtcga aaaggcgcag gctgccgtgc aggttccggc cgaggccgag
    12541 ttcggtgcca gcttgagtcg cttgatcccc ttcatggaac agttgatggc ttcgaaggga
    12601 cagcacaagt acttggtgat catcgatgag ttcgatgacc tcgatgccgc gttctacacg
    12661 ggagagcgag gcaagcagtt cgtcaagtct ttgcgctcga tctccgaagt cggcctgacc
    12721 ttcttctttg ttggtagtga gcggatggag gcaatctaca gccgccatca agcggacctg
    12781 aacaagtggc gaaacatcca actggaccgc atcgatagcc gcactgactg ccgagccttg
    12841 atcgcagaac cggtcgctaa cgtcatcgag ttctccaccg aggctgtgga cttcatcacg
    12901 gacttcacgg caggcaaccc cttctacatc cacaacttct gctaccaggt cttcgaacgc
    12961 tgcctgcagg agcatcgcac cttcgtggat gacaacgaca cgcacgctgt ccgcgatcag
    13021 ctgctgcgcg ccctcggtcc aacgaacttc tcacacctct gggaggacaa cccggtcctg
    13081 gagctagagg agaagcgtcg cgacagcgcg gagaactgcg tcacactggc ctgcatagct
    13141 gtgcttggag gtcgctatga agcggtcgag gatcttgcgg aagtccaaga gacgctgccc
    13201 attgctgtgg aggaccgcgc acccgccgcg gtactgcgga gggcatgtga acgacttacc
    13261 cggcgtggtg ttttgagcgt cgtgggcagc gggacagctc gcgtcatcag cctgcccatc
    13321 ttccgagagt ggctcggcgg aagtgccgtc gcaaagctgc tcccacagtg gagcgactac
    13381 aaggaggggt tgcgtcggac gcaggatcag gcgggctctg gactggaagc actcgaggta
    13441 cccgatcaga gcgcctttcc gatctcagag gacgatctgc tcgctatcac tcctcgtctg
    13501 gtctattgcg ggaagcagaa ggacgtcgct gagattcgct cttggctgcg gcagttcgac
    13561 gacgacagtc gcattgagat ggcgtttcag ctgctgcgac gagttgccga caaggggttc
    13621 atcaatgaag gcgccaagac cctggccctc aacgtcctgg agcagatgat ccaggagaag
    13681 cgccgtacga ccggcgaggg gaagtggcag gccatcaagg agcgtcgaga caatctcgcg
    13741 ctgggctact tcgattccga acacaagagc ggcgggagca cggcacgcga gctgcagaag
    13801 atccttcgac ctggcaagct gggtcccgcc attgggttgg aggcatggat gcgagcgcac
    13861 ttggaggcgg atgccctcct cgtcatcggc gacgactttg ctggcacagg gcaaacgctc
    13921 gcggaaggta tggccaagtt tcaggcgaag atcgatccgg ccatctggaa gcgctttgtc
    13981 gatgaacggc gcatcgccgt gtacgtcatg tttgcctttc cggaggctat taacaggatg
    14041 cgtgaggcgt tcccaggcat tgacgtcgtt gcggcgaccc tgctcggcga cgagctaaga
    14101 gcgtgcgacg agggggccgg gatctttgca gacgaagcag agcgccgctt cgctgaacaa
    14161 gttctccagc agatcggtcg tgaactgagt cccagggcgc caatggggca tggcgggcta
    14221 ggtgcgcttg tcgtcttcca caacgctgcg cccaacaaca gcttgccgat cctctggtgc
    14281 agcgggaccg taggcgagcg gccttggaag gcgctgtttc agcgagccta gacaacttga
    14341 tcggcagccg gtgcggcccc cattgcctcc aggatcgtcg acttgtggaa gcctgcgcca
    14401 gcagtgttct catgcagcct ctgtcgctcg cgtgagttgg gatcccgcgg aaggtgtcgc
    14461 tgattgctca cctgattgcc cgcttgctcg agcgcgcgaa cggcgggttc ggaggccgct
    14521 cccgcccgat cccaaagtcc gagcgaccga gtccggtctg tagcgccctt catctgcctg
    14581 ggcggccccg gaacagaaaa atggcgctcg acccgcaact agcgctccgc accgactgag
    14641 ctgtcgttca tcggtcagct gcagcacagg gatgaaggtg cacccgcgac cgtgtccagg
    14701 gcgggcgcag cccggcgcag cgcacacttg acgcggtcgc gggtgctata cgcttggctc
    14761 gcgggccgga ggcgccagcg tccggccccc gagcccgtcc ggcggcgagg acggccccgc
    14821 ccttggagcg gggagcgggg tggggatcaa cgatccattt cgcgatctgg ccgcgcacgc
    14881 tgcatcggca gtggctccgg cattgagcgc tggcgatcac gcgggacgac gtcacgctga
    14941 cggtttcgtt gaacctggcg tccgtaagtt gagtccttca cgaagcccgc gatgcgttcg
    15001 gccaagtcct ggtcctctgg acggtcggac ttgcgcagcg catcaaagat gttggcccag
    15061 gccaccaaag cattgctgcg attcttggcg taagcatcgc cggctttcga cgctggcgcc
    15121 tcgttcgaca gcctgccgtc ttctttcgcc ttcagctgcc acagctcctg tgatcgacgc
    15181 ccctcgccgc gcgtagcctg gcggctggct tctgcctcga tgcccagttc tcggagtcgc
    15241 tcggcgaaac tctcgcgcca ccgatgcagg tcggtcttgc gaggattcaa cctcgacccg
    15301 tccatgcccg tggcccggac gctcaggtgc acgtgcggat tggcctgatg gtcatgcagc
    15361 accattacgt accggtggcc gaccagttcg atcttcgcga atgcccgcgc tgcctgctgc
    15421 acggctaacg ggttggtgcc atgcggcatc gatagcatga tgttgaaggc ttcgcgccgg
    15481 tggctctctt cgggaatccg cgcgccgcca atgcgccatt gctcgaccag atcgagcatc
    15541 gactccttgc cgcggtacgt cagctcacgg tcgtcctcga tctgcagccg gccgttcttg
    15601 ctgatgtagc ggaagtgcgc tgcgatggcg ctcatgccgc gcccgccgcc cgtcaccttg
    15661 accatcacct gtggcgctcg ccggactaca gtggcgacga tgcgcccgcg gatcaccgca
    15721 gccttgcggc gcgtcagcgt gtcggggcgt ggcgtgggcg gtgtcttgac gatgcggttc
    15781 gacgggtaga acaggcgctc gccccactgg accagtacgc cgtcgacgat ctgtgcagcg
    15841 gtcatggccg tctcgcacgt ggcactcgtg gtgcgcggcg cgccgatgcc gcctgttgaa
    15901 gatcgatgag caactgcgac gccaggtcaa ggtgttgctg cacgcgggtc gatagcgacc
    15961 ggacgccggc gcgatagggc tcgagccggt catcgggcac ccgccccagc agccgcatga
    16021 agccgttgag atccataccc agcatggcca gctgatcggt cgagcgggac agggcagcaa
    16081 tcgcttgctg gcggtccggc gatgctgccg gcagggcgcg tccttcgagc agcgaagcca
    16141 cgtagatccc ttgcgacact tcggctcgtc gtgaccggct tgccagcaag agggcgtgtg
    16201 cagacggcaa tctcaccgtc accttcacga gcgctccatc ttctgtccga tcgacgccgg
    16261 caggccctgc agcctctgcc tcgggtgact cggccatggc gaccaccgcg cggcgcacga
    16321 gcgcggcgac ggtgaggccc tgcgcagcag cgcgttgctc aatcagcggc cctgcgcctc
    16381 gcaggtcgac ggtcaccctg tcgcggccgt ctcttgccat gtgatctgcc gccagacgtc
    16441 gaactgctct agggcgcgag cagcacgtcg agctgcctga ggcgaccgcg gagcgcctcg
    16501 agttgctccg cgggtagctg atcgaggccg acgcgtctca gctgcgagca ggcggccgcc
    16561 agttgggtac atagccgctc ggctcgcgcg accgactgca cacctgctgg cgcttcgagt
    16621 ggcttcttcg gcgctgcggg ccgcttcata gcgggcttcg acgcacgcgc tggccttcgc
    16681 agcgcggcga ctgcctcccg cgtgatcggg ttgtcgccct ccaggagatc gacgacggcc
    16741 tgggggcgat cggcgtgcac cctggccagc tcgtaaagcg tccttggtgc agcacatcga
    16801 ccggacttca aggcggctgc caacggcgcg ggcaggtcga gcagcgccag gtaatgcgcc
    16861 accgtcgtca ggtccatgcc gagtcgcttg gcgatggttg cgttcgattc gcccaggtcg
    16921 acgcgcccgc ggatgaagcg cgccaagtcc acaggcgtca gcccgtggcg cttctggttc
    16981 tcggcgacct gcgcatacgg atcggcgcca gtcggcctca ccgtgacggg cacctgctcc
    17041 agccccgcaa gacctgcagc gcggaaccgc ttggccccga agtggatgcg gtagagcccg
    17101 ccagcatccg caggctgcac gacgatcggc tgcaggatgc cgtgcaggcg gatgtcttcg
    17161 gccagctcct gcagctcggc cggcgggaac tcggtgcgcg ggttgtccgg atcctcacag
    17221 agcgcggaca gcggcacgaa cagcggctcg cctgtgggct gccgagatga ttcagtaacg
    17281 ggcacggagg gcacctcctg cggcagcgct tcaggctcgg ccatcggcgc ctgtcgcgat
    17341 gcatcgaagt ggacagctgg ctgctccgta gcgagcaggt ccaactgcga cggcttggaa
    17401 cggtcccagg gcaagcgcat gcgtcgatcg tgcgcgcggg ctcgccatct tccaagtgct
    17461 cgcgccagcg aggccttcgg tcccggtacg tctcgaatgc gctcacgggc gcccacaaga
    17521 tcccgagagg gttgtggttc ggtcacgcat cgtttcaact tcgaggctcg gcggcttcgc
    17581 cgtcacctca cagccttcgg ccaatggcgt gaaggtattg gccttggccg ataagttgat
    17641 gaaacgtgct gtagcttgtt tgttgcacgt acaggctttg gcttataaga tgtgccatgg
    17701 tcgctgcctt ccccgtccgc ttccctgatc aactccgcca gcatctgcgg gcgttgcgga
    17761 agcggcatgg cctgacccaa gcccaactgg gcgcactcgt cggtgtcagc caggcccgga
    17821 tcgctgagat cgaggcgaat ccaggcctgg tcaacttcga gcagctgatg aagctgctgg
    17881 ccgtgctggg tgtctccctg accctgcagg aggaagcggc cgtgccggtg gccacccgcg
    17941 tccctgctgc caagactgcg aagccaaagg ccaccaagcc aaggaccaag ccaccagcca
    18001 gaacgcctga ggtcgagccc gcctcgcgtc gacctctcgt ggcccggccc aagaagggct
    18061 cgtggtgaca cagtcgctca acgcctggat gaacggcgag ctggttggta cctggcgcgt
    18121 cgaccggaac tcgcacgcct tccgctacga cccgtcctgg ctgcagtcgc cccgccggcg
    18181 atcgctctcg ctctccctgc tcatcagtgg ctcgctggag attcagggcc agcaggtccg
    18241 caactacttc gacaacctgc tgccggacaa cgaacggatc cgctcgcggc tgcgaaggcg
    18301 cttcggcctc cgatcaagcg agatcttcga actgctggaa gccatcggtc gggactgcgt
    18361 cggcgccgtg cagctcctac ccgagggcgt cacgccggag gggtgggacc gaatcgattg
    18421 cgacccgctg accgaggcgc agatcgtcga cctgctccag gccgtgccgt cggagactga
    18481 ccccggctcg atccacgacg acgacctgtt ccggatctcc atcgccggcg cgcaggagaa
    18541 aaccgcgctc acccggtgga agggcagatg gtgccgaccg catggcgcca cgcctactac
    18601 ccacatcatc aagctcccgc tgggtctcat cggcggatcg aagcgggtcg atgcatcgga
    18661 ctcggttcag aacgagtggc tgtgcgcgca gatcatcgcg gagctcggct tgcccgttgc
    18721 ctcgacgtcg ttggagacct tcggcgacca gaccgtgctg atcgtcgagc gcttcgaccg
    18781 cgagtggatg gacaagggca agtggatcgc acgactgccc caggaagact tctgccaggc
    18841 gcttggcgtc gggccggacc agaagtacga gcaaggcggc ggccccggca tgcagaagtg
    18901 cctgcagctg ctgcagggca gtgccgaccg tgaggacacg acgctcttcc tgctcacgca
    18961 gctggcgttc gcactgatgg cggccaccga tggccacgcc aagaacttct cgatctatct
    19021 cgatcgaggc gatgcctatc ggatgacacc gctgtacgac gtcctgtcga tgtggcccta
    19081 ctacggcgac ggcgccaacc agttcaggcc gcgcaaggcg ggcctggcga tggccgtgcg
    19141 ctcgaagaac gcacactacc tgttccacac gatccaggca cgccactggc agcaactggc
    19201 ggccaggcac ggcgggaacg cggtgtgggc tgcgatggtc gaactcgtct ccagcgtcga
    19261 ggacgcgctc gctcgagtgg agcgacggct gcccaaggac ttcccggctc acacctggga
    19321 aagcatctcg acgggaatga ggagcgaggc caagcgcttc ctcagagagg caggactgct
    19381 ctagtgcgaa acctgcggtc gctctgcagt ccagttgtca acgtcgcttc gccgccagcc
    19441 cacggcacgc accgacagct ggaccggcga cgggaactgc cctgccgcga gcagccggta
    19501 gatcgtcgat cgacccagcc cggtcagacg catcacctcc ggcaggcgga ggatggcggg
    19561 tcggacggtt tccgtggcga gatgcacgtg ctgcatgcag catccctcgg tgatgtgact
    19621 ctgaacaatc tagtcgctca cgacacgcac cgagccgcat gatttcgaca gaaatctgtc
    19681 gctatgcctt gggactcaac cggaccacgg gcgcaccctg ccgaagcttg tccaggtagt
    19741 tcgcccacgc ctgcagcatc tttcggcgct gctccaggaa ctcggtccgg ttgtaggcgc
    19801 ggccaagcgg gtcgcgcacc gcgtgtgcga gttgagcctc aatgacgtcc gggtcgaagc
    19861 ctaggttctc ctgcagcatc gtccgggcag tcgcacggaa gccgtggccg gtcacctcat
    19921 cggctgagaa gcccatcgcc cgcagcgcgg cgttgatcgt gttctcgctc atggcgcggt
    19981 cgtgatgccg ctcaccgcgg aaaaccatct tcgcgtggcc ggtgagcgga tgcagctcac
    20041 gcagcacctc cactgcctga cgaggcagcg gcaccaggtg cggcgcgcca tggagcttct
    20101 gccgcagctc ccgcttcatg cggatcgccg gcacggtcca catcgccgca tcgaggtcga
    20161 tctccggcca ctccgcgaag cgcagttccc caggtcgcag caggaccatc ggcgccagct
    20221 tcaacgcggc gcgcaccacg ggtgtcgccg catagctgtc gatcgcgcgc agcagctcgc
    20281 ccaggcgttt gggctcggtg atggcgggga agtgcttggc catcggccgc cgcagtgcac
    20341 ccttcagatc acgagcgggg ttgctcggtg cctttccggt cgcaaccgcg tagcgcagcg
    20401 cctggctgca atcctgcagc gccctgtgtg cggtctcgag cacccctcgc gcctcgatcc
    20461 ggcgcatcac ctccagcagc agaggcgggg tcacgtcggc gaccggaacc ttgccgatgt
    20521 acgggaagac gtcgttctcg aggcgcgcga tgatccgctc gccatagctc ggcgaccagc
    20581 cgtccttctt gaccgcatac cactcgcgcg cgacggcttc gaatgagtct atcggcggca
    20641 ggccctgcgc ttcacgctcg tccgcgatcc gctcctgcgc ccaggactcc ttctcggcct
    20701 tgcgcttctg gctcgggtcg atgtcctccg cgacgagctg ccgcgcctca tccgccttgc
    20761 ggcgcgccag cgcgagcgac gtgtcgggat acgtgccgag cgagatcgtc ttgcgccgtc
    20821 cgtgatagct gtagtcgaag cgccaggcgt gcgcgccgcc cttcacgaac agcagcaggt
    20881 agagcccgtc accgtcgttg agtcgcttgc gcggatcacc cggccgcacg gccttgaggg
    20941 tggcatcgga agggatgaga tgacgggcca ttgcagtaac tgctgctttt cgcagcatcc
    21001 gcagccgagt tactgcccaa gttactgcac gaccggccgg atttcaagca acctgcagga
    21061 actcggcggg acatgaaaaa agccctagag cgttgattct ctagggcttt tgggacttcc
    21121 cgggacgtct ttggattccc tgggaacagt aacttggtgg ccaagggcgg aatcgaacca
    21181 ccgacacgcg gattttcaat ccgctgctct accaactgag ctacttggcc aagaaccgaa
    21241 gattatagcc gagcgccaaa cggcgcccgg gctcagagca ccccgccccg cttgttgcgc
    21301 gacaggtcca cgcccagctg cttgagcttg cggtacaggt gggtgcgctc caggccggtc
    21361 ttctcggcca cgc
//
LOCUS       DAOORV010000057        21235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3041_length_21235_cov_7.046034, whole genome shotgun sequence.
ACCESSION   DAOORV010000057 DAOORV010000000
VERSION     DAOORV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_21235_cov_7.046034"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKV56_09390"
     CDS             complement(<1..397)
                     /locus_tag="PKV56_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX68064.1"
                     /translation="MRSHVKKFSIALACLAALGSAVAADSKVLNVYNWSDYIADDTIK
                     NFEKETGIKVNYDNYDNNEVLHAKLVAGKTGYDIVVPGAHFAKAQIEGGLLQKLDRSK
                     LTNWGNLDKGLLEQLSKVDPGNEHLVDWLW"
     gene            610..978
                     /locus_tag="PKV56_09395"
     CDS             610..978
                     /locus_tag="PKV56_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HOX68065.1"
                     /translation="MSSRPQLDESRLHPAIRDTVEHLHAEVVHNVQAAAMSNAVLVVG
                     MATNPFCKRARKALDTAGVPHQYLEYGSYLSRWRERLALKMWTGWPTFPMVFVKGVLI
                     GGADDLAKLIASGELKRLLA"
     gene            1011..2504
                     /locus_tag="PKV56_09400"
     CDS             1011..2504
                     /locus_tag="PKV56_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX68066.1"
                     /translation="MAIALLAALSAAPKLLAQTTEVTAPCRMPGWANEVRCGVARRPL
                     DPARPGGAQLDIHYVVVRALARRRLPDPVFMLAGGPGQSAIALAPAVLPLFARLNNRR
                     DIVFVDQRGTGRSAPLDCDDNRLSPLSEPDGLDAQLARLKLCLARLATTAPLTSTDDL
                     KFFTTAIAMQDLDAVRARLGAERINLIGASYGTRAALDYARQFPARVRRSVLDGVAPP
                     DMVLPVSGGTDTQAAFDALLTDCERSPACHRSHPHLREEWTRLLASLPQDVLAAHPLT
                     GRTEHFTLDRDRLLGWVRGPLHAPWLAAALPRAIGEAAAGRHAGLLGLSAALSSRRNG
                     RVAMGMHFSVICAEDGPLLPPPDPAAAAATMPFVDHAMRLYQRACALWPRGAVPEDFY
                     RLPASSAPALLLSGGLDPATPPRHGERIARALGNKALHVVAPNAGHGLIGIGCVRDVV
                     FRFIDAMDDAAALAIDASCVTDIPRPPAFEPVDPSTSEAQPNESGAR"
     gene            2501..3298
                     /locus_tag="PKV56_09405"
     CDS             2501..3298
                     /locus_tag="PKV56_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX68067.1"
                     /translation="MIDVERLAKRFAPAPRSVGDLLARRPAPPGMRAVEEISFSAANG
                     CITGLLGPNGAGKTTTLRMLAGLIAPDAGRMSVDGIDVASHPREALARMGLLSDARGL
                     YPRLTARENIVYYGELQGLARDRAEARAEQLAGMLDLRPLLDRRTEGFSQGERMKIAL
                     ARALVHDPANIVLDEPTNGLDVPATRSLRELLRFLRTAEGGGKCIVFSTHIMQEVERL
                     CDHVVVVSQGRSVADGTVDELLARTQQTDFEEAFVQLAFPARSEECA"
     gene            3295..4458
                     /locus_tag="PKV56_09410"
     CDS             3295..4458
                     /locus_tag="PKV56_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68068.1"
                     /translation="MSAAWVVYVKEIVEALRDRRTLMVVLFSSVLMGPLLLVALSALL
                     ASFEASAESRVVYVAGSTHAPGLVNFLERQTRIVKPPPADYEARLRTRKWADPVLVVP
                     PDFEAALQRGETPIVEVVSDSGNAQAQAGAARVQRLLAAYLQERATLALALRGVSPEL
                     LAPFEVEERDLASSRARAAQITGMLPFFVIMAVLYGALNAALDTTAGERERGSLEPLL
                     MNPARRGSLVLGKWGAVASVAVLIATLSCFSFIPAQWLLRSDTLQAMFQYGWREALAF
                     LVVLLPLCAAMSALLMAIAIRCRTFKEAQASSTVALAIVSLLPLVTVFDQRGESPWHL
                     WVPALAQSTLMTRVLKGEPLGPAELAVPLAVSTLLTLMALAWMSRRLSDAALR"
     gene            4491..4970
                     /locus_tag="PKV56_09415"
     CDS             4491..4970
                     /locus_tag="PKV56_09415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX68069.1"
                     /translation="MTSKSAAFPLAQWRWCRFGDLHLHELQRIHTARQQVFIVEQRCP
                     YVDADGCDEHAFHLAAWVHGHPVPLAYARVVEPGFKYGEPSIGRVLTTAAGRGKGLGR
                     DLVQRAVALTIDAYPGLGIRISAQSHLEAFYRSFGFGAIGPGYLEDGIPHIEMLRPA"
     gene            complement(5009..5323)
                     /locus_tag="PKV56_09420"
     CDS             complement(5009..5323)
                     /locus_tag="PKV56_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4902 domain-containing protein"
                     /protein_id="HOX68070.1"
                     /translation="MDLRLIHMHTRVVQSGLHDTPCSGQTLWARVSSDVAAGVAWDWI
                     QLHQGVVAMADPLGLVTNLMLLDAQGEPIPTLQAALHLNEIVHALPWQTEVERALARI
                     AA"
     gene            5530..6243
                     /locus_tag="PKV56_09425"
     CDS             5530..6243
                     /locus_tag="PKV56_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HOX68071.1"
                     /translation="MLQSGFSSVFEAQSRDDLQTEIVRFARGLGFETVSATTVIDHFL
                     GQPEFISLDNAPAAYRDAFDDENNGRRDPVMQHCKKKSVPIIWDQTTYISAGEAEKWE
                     RQAHYGYRTGIALALHMPEGRHFLLGVDRDRPLPAGSQELTRMVADLQLFAVLAQDAA
                     LRVLLPAVPDATAVSLTPRELESLRWTMEGKTAWEVGNILGITERTAVLHLNNATHKL
                     DCVNKHQAVLKALRLKLIR"
     gene            6592..7878
                     /locus_tag="PKV56_09430"
     CDS             6592..7878
                     /locus_tag="PKV56_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOX68072.1"
                     /translation="MSGLFRTESVEHQSRSWLGGIQLVRPVPLALLTGLVACTAMATG
                     LFLFQGEYTRKAHVLGVIVPDRGLLRLPSPAVGTVVERRVSEGDPVRAGDVLFVVSVD
                     KSTLKGDAQSAVQGSLDARQRSLQEARAQSQALEREQVAALTRQIEDMRRELLQMDGE
                     AELQQQRLALALKAQARLESLQAENFVSSAHVQTRAEEVLGLRAQVVALARQRTAHQR
                     EIAALEALRREKPLQAQSQRGTIDRDLAELTQESAQSDALQRIVVRAPQTGVVTAVLA
                     APGDSVSPAAALASLLPEGARMQAELFAPSSAVGFLRPRQAVQLRYQAFPFQKFGHHP
                     GQVLQVSRTPLQAAELASLPLATGTTSAAPNSSARPPGEPLYRITVELDRQTVQAYGV
                     AQPLVAGMQLEADVLLDRRRLIEWIFEPLLSVSGRL"
     gene            7878..10043
                     /locus_tag="PKV56_09435"
     CDS             7878..10043
                     /locus_tag="PKV56_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOX68073.1"
                     /translation="MSNASAGFGATALSALHFGWGGHCMPMLLQTEEAECGLACLAMV
                     ATFHGLKTDLPTLRRRFSLSLKGSTLLDLTRMAGQLQLTPRALRAELDHLPQLQLPCV
                     LHWNLNHFVVLTALRGGKAVIHDPSHGIRHLSMNEVSKHFTGVALELTPTPDFRPREE
                     RQSVSVRQLLGRVTGLKRSLLQIMVLALALEVFMLLSPFFMQWVVDSVLVSADRDLLV
                     TLGLGFTLLVLVQVATGAIRSWAVLYLSSTLNLQWLSNVFAHLMRLPVVWFEKRQAGD
                     VMSRFGAVEKIQQTLTTSFIEVVLDGLLVVLTLAMMAFYSVKLTLVALGAVSIYALLR
                     WAFFQPLRDATEEGIVHDGRMATHFLESLRGVQSIKLFNRQEDRQAQFMNRAVDALNA
                     HILTRKLDLTLAVSHKLVFGLERVAVIWLGALLVLDRSFSVGMLFAFFAYKEQFAVRV
                     SGLIDKVVELRMLKLQGERLADIVLTAPEAEVAVPIDLGKVGGRIELGSIGFRYSDGE
                     PKVIDGLDLTIEPGESVAIVGPSGCGKTTLLKLMLGTLEPQTGEIRIGGLPLSRLGLR
                     AWRDMIGTVMQDDQLFAGSITDNISFFDASPDAAWVEECARLAAVHDEIEAMPMGFHT
                     LIGDMGTSVSGGQKQRILLARALYKRPRILFLDEATSALDVDRERLVNQAIRQLDLTR
                     VIVAHRTETIATAGRVIVLHEGRVAQDLRSVPGSWMAGR"
     gene            complement(10092..10391)
                     /locus_tag="PKV56_09440"
     CDS             complement(10092..10391)
                     /locus_tag="PKV56_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOX68074.1"
                     /translation="MQRRSTPTDLACRTGCGACCIAPSISSPIPGMPFIDGVGKAAGV
                     RCIQLTGDGRCALFGLPQRPAVCVSLRPSAEMCGETREQAIDWLTALERDTAPGP"
     gene            10507..12531
                     /gene="mnmG"
                     /locus_tag="PKV56_09445"
     CDS             10507..12531
                     /gene="mnmG"
                     /locus_tag="PKV56_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOX68075.1"
                     /translation="MWFPQVFDVIVIGGGHAGTEAALASARLGCATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMAAATDEAGIQFRILNGSKGPAVRATRAQADRV
                     LYRAAIRRRLENQPNLRLFQGACDDLIVDGDAVAGVVTQVGVRFRCRAVVLTAGTFLN
                     GKVHVGLDNHAAGRAGDPSAITLSSRLKELKLPQGRLKTGTPPRLDGRSIDFSRLTPQ
                     FGDGVPGTDGSPPETPMPVFSYLGEASQHPAQVPCWITHTNERTHEIIRSGFDRSPMF
                     TGVIEGVGPRYCPSIEDKVNRFADKTSHQIYLEPEGLTTHEFYPNGISTSLPFDIQLA
                     AVHSMLGLEHAHILRPGYAIEYDYFDPRALKASFETKSIRGLFFAGQINGTTGYEEAA
                     AQGLFAGLNAALQARDQPAWTPGRDEAYLGVLVDDLITKGVTEPYRMFTSRAEFRLQL
                     REDNADLRLTEHGRRLGLVDDARWESFSRKRDAVSRETQRLRTIWVRPQTLPATDAER
                     LLGKALEHEYSLSDLLRRPEVDHDLLSQIASIACTGPVVSRETLRIELGDTLADAVIE
                     QVEISAKYAGYITKQNDEIERAARYEHLVLPAELDYSQVSALSFEVRQTLSRHRPQTL
                     GQASRMSGVTPAAISLLLVHLKKAPFAGFIVPAEASGATEVIATPLADVA"
     gene            12587..13243
                     /gene="rsmG"
                     /locus_tag="PKV56_09450"
     CDS             12587..13243
                     /gene="rsmG"
                     /locus_tag="PKV56_09450"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOX68076.1"
                     /translation="MEPVAVPLDQLLAYMDLLQRWNATYNLTAVRDPQQMLIQHLADC
                     LAVVGPMKSLQLGQDSAVHGQVCPSLRILDVGSGGGLPGVVLASMFPGWRVVCVDTVG
                     KKAAFIRQAAAELGLGNLRAEHARVEALHAAPFDVITSRAFSSLLDFTRLTQKLLAPG
                     GVWMAMKGKVPADEISALPDEIEVFHVEPLVVPGLSAERCLVWMRPRPASSASSLNSH
                     "
     gene            13406..14176
                     /locus_tag="PKV56_09455"
     CDS             13406..14176
                     /locus_tag="PKV56_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68077.1"
                     /translation="MARIFCIANQKGGVGKTTTTVNLASGLSRVGQRVLVVDLDPQGN
                     ATMGSGIDKRALELSVYDVLLENASIPEVRQRNDKVGYDVIGANRELAGAEVELVELE
                     RRDKRLKSALAAVAAEYDFVLIDCPPSLSMLTLNGLCSAHGVIVPMQCEYFALEGLSD
                     LVNTIKQVHANLNPDLQIIGLLRVMFDPRITLQQQVSEQLKSHFGNKVFDTVIPRNVR
                     LAEAPSYGLPGVVFDPASKGAQAFVAFAQEMVQRIDSM"
     gene            complement(14213..15190)
                     /gene="argC"
                     /locus_tag="PKV56_09460"
     CDS             complement(14213..15190)
                     /gene="argC"
                     /locus_tag="PKV56_09460"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOX68078.1"
                     /translation="MKHRVFVDGQEGTTGLRIHEYLAQREDIEVLRIDPELRKETAER
                     ARLLNAADVAFLCLPDAASREAAALVTNPATCLIDASTAHRTAAGWAFGLPELAPGQR
                     AAIRASKRIANPGCHASAFILLIRPLVDAGLIPSDAPLTATSITGYSGGGKSMIRDYE
                     AAPLSAAAGCDAATPYVALAAPRPYALALAHKHLPEMKTHTGLAFPPVFMPIVGPFYK
                     GLTVSVPLHRSQLPDAVGAAELHAALERRYAGERFIRVMPLCDEATLAGGFFDVQAAN
                     DTNRVDLFVFASDTQVLLMARLDNLGKGASGAAVQSMNVHLGVDEGLGL"
     gene            15236..15811
                     /locus_tag="PKV56_09465"
     CDS             15236..15811
                     /locus_tag="PKV56_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX68079.1"
                     /translation="MTTDMGAPPGNAGGTDRRVLLLPGWQDSGATHWQSRWEALHGYR
                     RVQQDDWLWPRRGDWMTRLEETLLENPTPAVLVAHSLGCHLVAAWAAHSRHVARVHAA
                     LLVAPPDAERDDTPANLYSWRPIVRQRLPFRSLVVASSDDAYCSLARASGLAADWGSA
                     WCLLGARGHINSESQLGEWSEGHALLQPLMS"
     gene            15877..16809
                     /locus_tag="PKV56_09470"
     CDS             15877..16809
                     /locus_tag="PKV56_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX68080.1"
                     /translation="MVTKKPKGLGLGLEALLGPRVKEPTDAPAADADLAPRSLKLTQL
                     RPGKYQPRTRMDEGSLYELAESIKAQGIMQPILVRRVPDAASGSSTDAPQYEIIAGER
                     RTRAARLAGLDEVPVLVKDVPDEAAAAMSLIENIQREDLNPLEEAQGLKRLTDEFGLT
                     HEQAAQAVGRSRSAASNLLRLLNLVEPVQQLLMAGDLDMGHARALLPLDGARQILAAG
                     EIVARKLSVREAERLVARADDSGRQKPVKRVADAKSRDLQRIEQELSDALTARVEVRV
                     KKRTARGEQGEISIAFGSLDELNGLLDKLGLPKR"
     gene            17274..18350
                     /locus_tag="PKV56_09475"
     CDS             17274..18350
                     /locus_tag="PKV56_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX68081.1"
                     /translation="MNASTIAQPGLTSTPDDGTLATFAKAIMRSLAGRRDKPGTISSA
                     ETLCSRLDEASRTWTTHLGTAQAQMRQATEQLLSGFAQILEQLDAIIQPAHGAASGQP
                     VTASGLDSRAQVLEACESRLRGLIQNFQGFVQSRDAVMQSVRQLAGESVSLGTMAEDV
                     AQIARQTNLLSLNAAIEAARAGPSGRGFAVVATEVRRLSTESGDTGKRIGDHVQAFGQ
                     HMNAALKQASEHTAHDAQVIHASEQTINQVIEQVDSAVSQLNERAAELSARGEAVRAQ
                     VEQLMIAFQFQDRVHQIMDQVNSSISSAVARLQQSLKDGAAPDADEWTALLSAGYTTD
                     EQRFASDHGSQNAAAPSSNDTTFF"
     gene            18400..18771
                     /locus_tag="PKV56_09480"
     CDS             18400..18771
                     /locus_tag="PKV56_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX68082.1"
                     /translation="MAKTILIVDDSSSLRTVVRMALVRAGYDVIEAGDGKEGLAQLDK
                     AGKVNLIVSDVNMPNMDGITFVTHVKAHPRHKFTPVVMLTTEGQDAKKEQGRAAGAKA
                     WIVKPFNPPQLLDAVSKLILP"
     gene            18781..19092
                     /locus_tag="PKV56_09485"
     CDS             18781..19092
                     /locus_tag="PKV56_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX68083.1"
                     /translation="MTHTLSLPAELTIYTVGELRPQWLSWLAGFAADDAPADASVDGS
                     AVDQIDAAGVQLLMALSRSLAAAQRSLQFLSVSPVLATACAALGLPVSMAAPDDQAGE
                     A"
     gene            19089..>21235
                     /locus_tag="PKV56_09490"
     CDS             19089..>21235
                     /locus_tag="PKV56_09490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOX68084.1"
                     /translation="MKQQQDLDNSFIAEALPAFISEAQEQLEMLEQLLLQLEDAPQDS
                     ELLGALFRCAHTVKGSAGIFGLDEVVAFTHHVETLLDRLREGRVALTPQLSTLLLQCN
                     DQIRALIAAAQDPQADDASAAEARAALVLRLQAASGDGAPDAAAPEEASAPAATLAAG
                     VRRWHLWVKFGEDTYRNGMDPLAILTYVRGLGSIPSLVCDLADVPTLESIDPENCHLG
                     FEFSLETEASRPEIEAAFSFVREDCTLHVTEPNATAKDFARLIESMPDNQRLGDILVS
                     VGAISRVQLEQALREQAAASANGNAQALGEILQAKSGLSPKLVDAALQKQNKQREGGS
                     TAPAASDDNRFIRVQADRLDAVINLLGELVIAGAGASLLARQTRQGALIEATAQIGSL
                     VEEIRNGTLQLRMVPIGETFSRFRRVVRDTAAELGKDVALEIVGGETELDKSVVERIA
                     DPLMHLVRNALDHGLETPEQRAEAGKPAQGRLTLSACHESGSILIRIVDDGRGIQRER
                     VLQRAWDRGLVEQGVTPPEADILKLIFEPGFSTAEKVTNLSGRGVGMDVVRSNIQALR
                     GSVTLTSTEGQGSTIEIRLPLTLAIIDGFLIGVGNSKFIFPLDAVVEVIANRARPEQL
                     DERGRGVVELRGQVLPVVSLRALYALEGDEPARGSVVVLQAGSRRYGVLVDQLLGQHQ
                     TVIKPLGRMFRSLRGMSGSSILGNGEVALIFDV"
BASE COUNT         3631 a         7106 c         7040 g         3458 t
ORIGIN      
        1 cccacagcca gtcgaccagg tgctcgttgc ccgggtcgac cttcgacagt tgctcaagca
       61 ggcccttgtc gaggttgccc cagttcgtca gcttgctgcg atcgagcttc tgcagcagac
      121 caccctcgat ctgagccttg gcgaagtgcg cgcccggcac cacgatgtcg tagccggtct
      181 tgccagcgac cagtttcgcg tgcagcacct cgttgttgtc gtagttgtcg tagttgacct
      241 tgatgccggt ttccttctcg aaattcttga tcgtgtcgtc ggcgatgtag tcggaccagt
      301 tgtaaacatt gagcaccttc gagtcggcag caaccgcgct gcccagcgcg gcgaggcagg
      361 ccagggcgat gctgaatttc ttgacgtgac tgcgcatgac gttgtgatcc ttgtcttgtc
      421 gggttggaat cgaggagagc ggcgaacggt tcccgccctg aagcaggaac cgagccgtcg
      481 ccgcggcgag gaattctgta cgagaaccag ggtaacggag cctgacggat tctcgatgac
      541 cgacatcgcg ctcggctacg cttgtgacct gtcgccaccg cccgcggcgg ccccctgatg
      601 gagccatcca tgtcctcccg tccccagctc gatgaatccc ggctgcaccc ggccatacgc
      661 gacacggtcg aacacctgca tgccgaggtg gtgcacaacg tccaggcggc tgccatgtcg
      721 aatgcggtgc tggtcgtcgg catggcgacc aatccgttct gcaagcgggc ccgcaaggcg
      781 ctcgacacgg ccggcgtgcc gcaccagtac ctggaatacg gcagttacct gagccggtgg
      841 cgcgagcgcc tggcactgaa gatgtggacc ggctggccga cgttcccgat ggtgttcgtc
      901 aagggcgtgc tgatcggcgg cgccgacgac ctggcgaaat tgatcgccag cggcgagctc
      961 aagcggctgc tcgcttgagt caaggtcgac cgggggctcg ctggcggtcg atggcgatcg
     1021 ccctgctggc agccctgagc gcggcgccga agctgcttgc gcagaccacc gaggtgacgg
     1081 caccctgccg catgccggga tgggccaacg aggtgcgctg cggcgtggcg cggcgaccgc
     1141 tcgaccccgc gcggccgggc ggcgcacaac tcgacatcca ctacgtggtg gtgcgtgcac
     1201 tggcccgccg ccgcctgccc gacccggtct tcatgctggc cggcgggccg ggccagagtg
     1261 cgatcgcgct ggccccggcc gtgctgccgc tgttcgcgcg gctcaacaac cggcgcgaca
     1321 tcgtcttcgt cgatcagcgc ggcaccggcc gctcagcacc gctcgattgc gacgacaacc
     1381 gcctctcccc cttgtccgaa ccagacggac tcgatgcgca actggcgagg ctgaaactct
     1441 gtctcgcacg gctggcgacc accgcaccgc tgacttcgac ggacgacctg aaattcttca
     1501 ccacggccat cgcgatgcag gatctggatg cggttcgcgc gagactgggg gccgagcgca
     1561 tcaacctgat cggcgcgtcg tacggcacac gtgcagcgct cgactacgcg cgccagtttc
     1621 cggcccgggt acgacgcagc gtgctcgacg gcgtggcacc gcccgacatg gtgcttccgg
     1681 tgagcggtgg caccgacacc caggccgcct tcgacgccct gctgaccgat tgcgagcgca
     1741 gtccggcctg ccatcgcagc cacccgcatc tgcgcgagga atggacccgc ctgcttgcca
     1801 gcctgccgca ggacgtgctg gcagcgcatc cgttgaccgg ccgcacagag cacttcacgc
     1861 tcgatcgcga tcggctgctg ggatgggtgc gcgggccact gcatgcaccc tggctggcag
     1921 ccgcactgcc acgcgccatt ggcgaggcag cggcaggccg ccacgcaggg ctgctgggcc
     1981 tgtcggccgc actgtcgagc cgacgcaatg gccgggtggc catgggcatg catttcagcg
     2041 tgatctgtgc ggaagacggc ccgctgctgc ctccgcccga tcccgcagcg gcagccgcca
     2101 cgatgccgtt cgtggatcac gccatgcggc tgtaccagcg cgcctgcgcg ctctggccgc
     2161 gcggcgcggt gccggaggac ttctatcgcc tacccgcttc gtcagcacct gcgctgctgc
     2221 tgagcggagg gctggatccg gccacgccgc cgagacacgg agaacggatc gcccgcgcgc
     2281 tcgggaacaa ggcattgcac gtggtcgccc ccaacgctgg ccacggcttg atcgggatcg
     2341 gctgtgtgcg cgatgtagtg ttccgcttca tcgatgcgat ggacgacgct gcagcgctgg
     2401 ccatcgatgc gtcgtgcgtc accgacatcc cgcgtccgcc cgcattcgag cccgtggatc
     2461 cgtcgacgtc cgaggcgcaa cccaacgaat cgggcgcaag gtgattgacg tcgagcgact
     2521 ggccaagagg tttgcgccgg ccccgcgaag cgtgggtgac ttgctggccc gacgcccggc
     2581 gccgcccggg atgcgcgccg tcgaggagat cagcttcagc gcggccaacg gatgcatcac
     2641 cgggctgctg ggacccaatg gcgccggcaa gacgaccacg ctgcgcatgc tggccggact
     2701 gatcgcgccg gacgcggggc gcatgtcggt cgacggcatc gacgtggcgt cgcacccgcg
     2761 cgaggcactg gcgcggatgg ggctgctcag cgatgcccga ggcctctacc cacggctgac
     2821 tgcacgcgag aacatcgtct actacggcga attgcaaggt ctggctcgcg accgcgccga
     2881 ggcgcgggcc gagcagctgg caggaatgct cgacctgcgg ccgctgctcg accgccgcac
     2941 cgaaggattc agccagggcg aacggatgaa gatcgcgctg gctcgggccc tggtgcacga
     3001 cccggcgaac atcgtgctgg acgagccgac caacggcctt gacgtgcccg ccacacgcag
     3061 cctgcgcgaa ttgctgcgtt tcctgcgcac ggccgagggg ggaggcaagt gcatcgtgtt
     3121 ctcgacgcac atcatgcagg aggtcgagcg gctgtgcgac cacgtcgtcg tggtgtcgca
     3181 agggcgcagc gtcgccgacg gcacggtcga cgagctgctc gcccggacgc agcagaccga
     3241 tttcgaggag gcgttcgtgc aactggcgtt cccggccaga agcgaggagt gcgcatgagc
     3301 gccgcctggg tcgtctatgt gaaggaaatc gtcgaggcgc tgcgcgaccg ccgcaccctg
     3361 atggtcgtgc tgttcagcag cgtgctgatg ggtccgctgc tgctggtcgc gctgtcggcg
     3421 ctgctcgcaa gcttcgaggc cagcgcggag agccgggtcg tctacgtcgc gggcagcacg
     3481 catgcacccg gcctggtcaa cttcctcgag cggcagaccc ggatcgtgaa gcccccaccg
     3541 gcagattacg aggctcggct gcgcacacgg aaatgggcgg atccggttct ggtcgttccg
     3601 cccgacttcg aggcggcatt gcaacgcggc gagacgccga tcgtcgaagt ggtcagcgac
     3661 agcggcaacg cgcaggcgca ggcgggtgcg gcccgcgtgc agcgcctgct ggcggcctac
     3721 ctccaggagc gagccacgct cgctctggcc ctgcgtggcg tatcgccgga gttgctggcc
     3781 cccttcgagg tggaagagcg cgatctggcg agttcgcgcg cacgtgctgc gcagatcacc
     3841 ggcatgctgc cgttcttcgt gatcatggcg gtgctgtacg gcgcactgaa tgctgcactc
     3901 gacacgaccg ccggagagcg cgaacgcgga tcgctggagc cgctgctgat gaacccggcc
     3961 cgacggggat cgctggtact gggcaagtgg ggcgcggtcg cgagtgtcgc cgtgctgatc
     4021 gcgaccctga gctgcttcag cttcatacct gcgcaatggc tgctgcgcag cgacacgctg
     4081 caggccatgt tccagtatgg ctggcgcgag gcactcgcgt ttctggtggt gctgctgcct
     4141 ctctgtgcgg ccatgtcggc gctgctgatg gcgatcgcga tccgctgcag gaccttcaag
     4201 gaggcgcagg ccagcagcac ggtcgcgctg gccatcgtgt cgctgttgcc actggtcacg
     4261 gtcttcgacc agcgcgggga atcgccctgg cacctgtggg tgcctgcgct ggcgcaatca
     4321 accctgatga cgcgcgtcct gaagggcgaa cccctcggcc ccgcagagct ggccgtaccg
     4381 ttggcggtgt caacgctgct gacgttgatg gccctggcct ggatgtcgcg gcggttgagc
     4441 gacgccgcgt tgcgatgaga agcgcagcga cttcggggca tgatgcgtcg atgacctcga
     4501 agtccgccgc ctttcctctt gcgcaatggc gctggtgccg attcggcgat cttcatctgc
     4561 acgagctgca gcgcatccac acggcgcgcc agcaggtttt catcgtcgag caacgctgcc
     4621 cctacgtcga tgccgatggc tgcgacgagc acgccttcca tctggccgcc tgggtgcacg
     4681 gacacccggt gccgctggcc tacgcgcgag tcgtcgagcc gggcttcaag tatggggagc
     4741 cctcgatcgg tcgcgtgctg accacggcgg ccggtcgcgg caaggggctg gggcgggatc
     4801 tggtgcagcg tgcggtggcg ctgacgatcg atgcgtaccc gggcctgggc atacgcattt
     4861 cagcgcagag ccacctggaa gccttctatc gaagcttcgg attcggtgca atcggcccag
     4921 gctatctcga ggatggcatc ccgcacatcg agatgctgcg gccggcctga cgggctgggc
     4981 ccttgtctcg accaagctgc atcgtcgatc acgcagcaat gcgcgccaga gcccgctcga
     5041 cctcggtctg ccagggcagg gcatggacga tttcgttgag atgcagcgcc gcctgcaggg
     5101 tgggaatcgg ctcgccctgc gcgtcgagca gcatcaggtt ggtaaccaat cccaacgggt
     5161 ctgccatcgc gacgacgccc tggtgcagct ggatccagtc ccaggcgaca ccggccgcca
     5221 cgtcactgct gacccgtgcc cacaaggtct ggccggagca aggcgtgtca tgcaggcccg
     5281 actgcacgac ccgggtatgc atgtggatca aacgcaaatc catcgtagag tccgcctgcc
     5341 acaatagggg aggccatgcg cgcactgtcc acgggggagc catcggtagt accgccttcc
     5401 agaaggcagt caccgaactg ccatggatgc cagtgtggtc cggttcgcca agcttgccac
     5461 ctgtcaatgt tttcaggtgt gtgcgagtga gacagcgaga acgaggcttt cgggagtgat
     5521 cggtttctga tgcttcagag cggcttttca tcggtgttcg aggcgcagag ccgcgacgat
     5581 ctccagacgg aaatcgtccg ctttgcccgc ggcctcggct tcgagacggt gtcggcgacc
     5641 accgtgatcg accatttcct gggccagccc gaattcatct cgctcgataa cgcgccggcg
     5701 gcctaccggg acgccttcga cgacgagaac aacggtcggc gcgacccggt gatgcagcac
     5761 tgcaagaaga agagcgttcc catcatctgg gatcagacca cctacatctc ggccggagaa
     5821 gccgagaaat gggagcgaca ggcccactac ggctatcgca ctggcattgc gctggcgctg
     5881 cacatgcccg aggggcgcca tttcctgctc ggtgtcgacc gcgatcggcc attgcccgcg
     5941 ggctcgcagg agctcacaag gatggttgcc gacctgcagc tgttcgccgt gctggcccag
     6001 gacgcggcgc tgcgggtgct gctgccggcc gtccccgatg ccaccgcggt gtcgttgact
     6061 ccccgcgagc tcgaaagcct gcgctggacc atggaaggca agaccgcctg ggaggtgggc
     6121 aacatcctcg gcatcacgga gcgtaccgcc gtgctgcacc tgaacaacgc cacccacaag
     6181 ctcgactgcg tgaacaagca tcaggcggta ctgaaggcac tgcgactgaa gctcatccgc
     6241 tgactcgggc aagccggtcg gctaacgaca agccggccgt caggcaagct gtaagagttg
     6301 acagttacgg ccgcgggggc ccgctgctga aatttggatg tgcacatgtt gtgcatccat
     6361 ctcttcagga gcttcaaatg cggccactgc cccaaaacat cccggcgccc cgtcaaaacc
     6421 ctgcacctgc ccgcgtcgac gcacaacgcg atgcgcgccg cgcagctccg acagcccctc
     6481 gcgagctgga tgccgatgaa ctgcgccatg tgagcggcgc tggcacgcga ctgccgaacc
     6541 ggacctggtg aatcgcaccc ggcccgtccg ggggcggtcc tgcgctgagc gatgagcggg
     6601 ctctttcgca ccgagtccgt cgaacaccag agccgctcgt ggctgggcgg catccagctc
     6661 gtgcgccccg tcccgctggc gctgctgacc ggactggtgg cctgcaccgc catggcgacc
     6721 gggttgttcc tgttccaggg ggagtacacc cgcaaggccc atgtgctggg ggtgatcgtc
     6781 cccgaccgag gcctgctgcg actgccctcg ccagcggtcg gcacggtcgt cgagcggcgg
     6841 gtgagcgaag gcgatccggt tcgcgctggt gacgtgctct tcgtcgtttc ggtggacaaa
     6901 tcgacgctca agggcgacgc ccagtctgcc gtgcagggca gcctggatgc tcggcagcgc
     6961 agcctgcagg aggcacgcgc gcaaagccag gctctcgaac gggagcaggt cgctgcgctg
     7021 acccggcaga tcgaggacat gcgccgcgag ctgctgcaga tggatggcga ggccgaactc
     7081 cagcagcagc gcctggccct ggcactgaag gcccaggccc ggctggaatc attgcaggcc
     7141 gagaacttcg tttcttcggc tcacgtacag acccgggcgg aagaggtgct cggcttgcgg
     7201 gcgcaggtcg tggcgctggc ccgacagcgc acagcccacc agcgcgaaat cgccgcgctc
     7261 gaagcgctgc gccgggaaaa gccgctgcaa gcgcaatcac agcgcggcac catcgatcgc
     7321 gatcttgcgg agctgacaca ggagtcagcc cagagcgatg cgttgcagcg catcgtggtt
     7381 cgtgccccgc agacgggcgt cgtcacggca gtgctcgcgg cccccggaga cagcgtatcg
     7441 ccggcagcgg cgctcgccag cctgctgccg gaaggtgccc ggatgcaggc ggagctgttc
     7501 gctccgtcga gcgcggtggg cttcctgcgg ccccggcagg cggtgcagtt gcgctaccag
     7561 gccttcccgt tccagaagtt cggccaccat ccaggtcagg tgctgcaggt gtcgcgcaca
     7621 ccgttgcagg cggccgagct cgccagtctg ccgctggcaa cgggaacgac ttcggccgca
     7681 ccgaacagca gcgcgcgccc tcccggcgaa ccgctctacc gcatcaccgt cgaactcgac
     7741 aggcagactg tccaggccta cggcgtcgct cagccactgg tcgccggcat gcagctcgag
     7801 gccgacgtgc tgctcgaccg ccggcgactg atcgagtgga tcttcgagcc gttgctcagc
     7861 gtgagcgggc gtctgtgatg agcaacgcca gcgcgggatt cggcgcgacg gcactctcgg
     7921 cgctgcactt cggctggggt ggccactgca tgcccatgct gcttcagacc gaggaggctg
     7981 aatgcggcct ggcctgtctg gcgatggtgg ccacgttcca cgggctgaaa accgatctgc
     8041 cgacgttgcg gcgccggttc tcgctgtccc tgaagggatc gacgctgctg gacctgacac
     8101 gcatggcagg ccagttgcag ctgacaccgc gcgcgctgcg cgccgagctc gatcatctgc
     8161 cgcagctgca actgccctgc gtgctgcact ggaacctgaa ccacttcgtc gtgctgacgg
     8221 ccttgcgcgg cggcaaggcg gtgatccacg acccgtcgca cggcatccgc cacctgtcga
     8281 tgaacgaagt gtcgaaacac ttcaccggcg tcgcgcttga attgacgcca acacccgact
     8341 tccgcccgcg tgaagaacgg cagtcggtgt cggtgcggca gctgctgggt cgggtcaccg
     8401 ggctcaagcg atcactgctg cagatcatgg tcctggcact ggcgctcgaa gtgttcatgc
     8461 tgctgtcgcc gttcttcatg cagtgggtgg tcgacagcgt gctggtcagt gccgatcgcg
     8521 acctgctggt gacactcggg ctgggcttca cgctgctggt gctcgtgcag gtggccaccg
     8581 gcgcgatccg ctcctgggcc gtgctctacc tgtcgtcgac cctgaacctg cagtggctgt
     8641 cgaatgtatt cgcgcatctg atgcgcctgc cggtggtctg gttcgagaag cgacaggcag
     8701 gcgacgtgat gtcgcgcttc ggcgcggtcg aaaagatcca gcagacgctg accaccagct
     8761 tcatcgaggt cgtgctcgac ggacttctgg tcgtgctgac cctggcgatg atggctttct
     8821 acagcgtcaa gctgaccctg gtcgctctcg gagcggtctc gatctacgcg ctgctgcgct
     8881 gggccttctt ccagccgctg cgcgacgcga cggaggaagg gatcgttcac gacggtcgga
     8941 tggcaacgca cttcctcgag tcgctgcgtg gggtgcagtc gatcaagctg ttcaatcgac
     9001 aggaggaccg gcaggcgcag ttcatgaacc gcgctgtcga cgcgctgaac gcgcacatcc
     9061 tgacccgcaa gctcgacctg accctggcgg tgtctcacaa gctggtgttc gggctggagc
     9121 gcgtggccgt gatctggctc ggggcgctgc tggtgctcga ccgcagtttt tcggtgggca
     9181 tgctgttcgc cttctttgcc tacaaggaac agttcgcggt tcgggtcagc gggctgatcg
     9241 acaaggttgt cgagttgcgg atgctgaagc tgcagggcga gcggctcgcc gacatcgtgc
     9301 tgaccgctcc cgaggccgaa gtggccgtgc cgatcgacct cggaaaggtc ggcggccgga
     9361 tcgagctcgg cagcatcgga ttccgctatt ccgacgggga acccaaggtc atcgacggcc
     9421 tcgacctgac gatcgagccc ggagaatcgg tggcgatcgt cggaccatcg ggttgcggca
     9481 agacgacgct gctgaaactg atgctcggca cgctcgagcc ccaaacgggc gaaatacgca
     9541 tcggcggcct tccgctgtcc cgtctgggcc tgcgcgcatg gcgcgacatg atcggcacgg
     9601 tgatgcagga cgaccagctg ttcgccggct cgatcaccga caacatcagc ttcttcgatg
     9661 cctcacccga cgcagcctgg gtcgaggaat gcgcccggct ggccgcggtg cacgacgaga
     9721 tcgaagcgat gccgatgggc tttcacacgc tgatcggcga catgggcacc agcgtgtccg
     9781 gcgggcagaa gcagcgcatc ctgctggctc gcgcccttta caagcggcca cggatcctgt
     9841 tcctcgacga agccaccagc gcgctcgatg tggaccgcga gcggctggtc aaccaggcga
     9901 ttcggcagct cgacctgacc cgcgtgatcg tggcgcaccg caccgagacc atcgccaccg
     9961 cgggccgggt gatcgtgctt catgagggcc gggtggcgca ggacctgcgc agcgtcccgg
    10021 gaagctggat ggccgggcgc tgacggcctg accggcggca aatcgattca gtctgcgcgc
    10081 ttcagggccg actacggtcc gggcgcggtg tcgcgctcga gcgcggtgag ccagtcgatg
    10141 gcctgctccc gcgtctcgcc gcacatttcc gcgctcgggc gcagcgacac gcacaccgcc
    10201 ggccgttgcg gcaatccgaa cagcgcgcag cgcccatcac cagtcaactg gatgcaccgc
    10261 acgcccgccg ccttgccgac gccatcgatg aacggcatgc cggggatcgg actggagatc
    10321 gacggcgcga tacagcaggc accgcagccg gtgcgacagg ctagatcggt cggggtcgat
    10381 cgtcgctgca tggaagggtg gagcgtgaca ttgggtggcg tgggcggcag gggttcaggc
    10441 gccttggggt gacttcgctg aggtgcctcc ctacaatccc ggattgctca aaaaacaggc
    10501 agcgtcatgt ggttccccca agtcttcgat gtcattgtga tcggtggcgg tcatgccggc
    10561 accgaggcgg cactcgcatc ggcacgcctc ggctgcgcga ccttgctgct gacgcacaac
    10621 atcgagacgc tggggcagat gagctgcaac ccgtcgatcg gcggcatcgg caagggccat
    10681 ctggtcaagg agatcgatgc gctgggcggt gcgatggccg cggccaccga cgaggcagga
    10741 atccagtttc gcatcctcaa cggctcgaag ggcccggccg tgcgtgcaac gcgcgctcag
    10801 gccgaccggg tgctgtaccg cgcggccatc cgccggcggc tggagaatca gccgaacctg
    10861 cggctgttcc agggagcttg cgacgatctg atcgtcgatg gtgatgctgt agcaggggtt
    10921 gtgacgcaag tgggcgttcg cttccggtgt cgtgccgtgg tgctgactgc cgggacattc
    10981 ctcaacggca aggtccatgt cggcctggac aaccacgcgg ccgggcgggc gggggatcca
    11041 tccgccatca cgctgtcatc tcggctgaag gagctgaagc tgcctcaggg ccgcctgaag
    11101 accgggacac caccgcggct cgatggacgc agcatcgatt tcagccggtt gacgccacag
    11161 ttcggtgatg gggtcccggg caccgacggc agcccgccgg agacaccgat gccggtgttc
    11221 agctaccttg gcgaagcttc gcagcatccg gcgcaggtcc cttgctggat cacgcacacc
    11281 aacgagcgaa ctcacgagat catccgctcg ggtttcgacc gcagcccgat gttcaccggc
    11341 gtgatcgaag gggtggggcc gcgctactgt ccgagcatcg aggacaaggt caatcgtttt
    11401 gccgacaaga cgtcgcacca gatctacctc gagccggaag ggctgaccac acacgagttc
    11461 tatccgaacg gcatctcgac ctcgctgccc tttgacattc aactggccgc cgttcactcg
    11521 atgcttggcc tcgagcacgc gcacatcctg cgaccgggct acgcgatcga atacgactac
    11581 tttgacccgc gcgcgctgaa ggccagcttc gagaccaagt cgatcagagg cctgttcttt
    11641 gccggtcaga tcaacgggac gaccggatac gaggaggcgg ccgcacaggg tctgttcgca
    11701 ggtctgaatg ctgcgctgca ggcgcgcgac cagcccgcct ggacgcccgg tcgggacgag
    11761 gcctacctgg gcgtgctggt ggacgacctg atcaccaagg gcgtcaccga gccgtatcgc
    11821 atgttcacca gccgggccga gttccggctg cagctgcgcg aggacaacgc cgacctccgg
    11881 ctcaccgagc acggccgccg ccttggcctg gtcgatgacg cccgatggga gtcgttcagc
    11941 cgcaagcgtg atgcagtttc acgtgaaaca caaaggcttc ggacaatctg ggtccgcccg
    12001 cagaccctgc ctgccaccga cgccgagcga ctcctgggca aggcactcga acacgagtac
    12061 agcttgtcgg atttgctgcg aaggccggaa gtggatcacg acttactgag ccagatcgcg
    12121 tccatcgcct gcacgggacc cgtcgtttca cgtgaaacac tgcgcatcga gctgggcgac
    12181 acattggccg acgcggtcat cgagcaggtc gagatcagcg ccaagtacgc cggctacatc
    12241 accaagcaga acgacgagat cgagcgtgct gctcgctacg agcatctggt gctgccggcc
    12301 gaactggact attcgcaggt gtccgcgctg agtttcgagg tcaggcagac gctgagccgc
    12361 caccgcccgc agacgctggg gcaggcttcg cggatgtctg gcgtcacgcc agcggcgatt
    12421 tccctgctgc tcgtgcacct gaagaaggct ccctttgccg ggttcatcgt acccgcagaa
    12481 gcttccggtg ctaccgaggt catagcaact cccttggccg atgtcgcctg aacactcgct
    12541 gcatccgctg cacggaccgc tcgcgcgcat cgtcaccgag ctcggaatgg agccggtagc
    12601 ggttccgctg gatcagcttc tggcctacat ggacttgctg cagcgctgga atgccacata
    12661 caacctgacg gcggtgcggg atccgcagca gatgttgatc cagcatctgg ccgactgcct
    12721 ggcggtggtg ggaccgatga agtcgctgca actcggtcag gactccgcgg ttcacgggca
    12781 ggtctgtccc tcgctgcgta tcctcgatgt gggcagcgga ggcggtttgc ccggcgtggt
    12841 actggcgtcg atgttcccgg gatggcgggt tgtttgtgtg gacaccgtgg gcaagaaggc
    12901 cgcgttcatc cgtcaggccg ctgcggagtt ggggctgggc aacctccgtg cagagcacgc
    12961 ccgggtcgag gctttgcatg ctgccccctt tgacgtcatc acgtcgcggg ccttttcgtc
    13021 cttgctggat ttcacccgcc tgacccaaaa gctgcttgca ccgggcggtg tgtggatggc
    13081 catgaaaggc aaggtgcccg cagacgagat ttcggcgctg ccggacgaga tcgaagtgtt
    13141 tcacgtggaa ccactggtcg taccggggtt gagcgccgag cgatgcctgg tctggatgcg
    13201 tcctcgcccg gcatcctcag ccagttcgct gaatagccac tgatcgccgt cacaaggact
    13261 gtcgtgggcc gcacttcggg agcttccgaa gcagggcgcg ctcaacccgg ttcctataat
    13321 cgaagacgct gccacggaca tggaagcagg ccttcctgcg ctgtcagcga cctttcctct
    13381 gcatccgacc ccggacgttc aacccatggc ccgaattttc tgcatcgcca atcaaaaagg
    13441 cggggttggc aagaccacga cgaccgtgaa cctggcctcc ggcctgtcac gcgtcggtca
    13501 gcgtgtgctg gtggtcgatc tcgaccctca gggcaatgcg accatgggat cgggcatcga
    13561 taaacgggcc ctcgaactga gcgtctacga cgtgctgctc gagaacgcct cgatcccgga
    13621 ggtccgtcag cgcaacgaca aggtcggcta cgacgtgatc ggtgccaacc gggagctggc
    13681 cggtgccgag gtggaactgg tcgaactgga gcgccgcgac aagcggctca aatccgcact
    13741 ggctgccgtc gccgccgagt acgacttcgt gctgatcgac tgcccgccgt cattgagcat
    13801 gctgaccctg aacggcctgt gcagcgcgca cggagtcatc gttccgatgc agtgcgagta
    13861 ctttgcgctt gagggtctgt cggatctggt caacaccatc aagcaggtgc atgccaacct
    13921 gaatcccgat ctgcagatca tcggtctgct gcgggtgatg ttcgacccgc gcatcacgct
    13981 gcagcaacag gtcagcgagc agctcaagtc gcacttcggc aacaaggtgt tcgacacggt
    14041 gattccgcgc aacgtgcggc tggccgaagc tccgagctac gggcttcccg gagtcgtctt
    14101 cgatccggca tcgaagggcg ctcaagcctt cgtcgccttc gcgcaggaga tggttcagcg
    14161 catcgattcg atgtgagtat tcgcttactt cacgaatgaa cctcacgccg gattacagtc
    14221 ccagcccctc gtcgacaccg aggtgcacat tcatcgactg aacggccgca ccgctggccc
    14281 ctttgcccag gttgtccaat cgcgccatca acagcacctg cgtgtcgctc gcaaagacga
    14341 acagatcgac ccggttggtg tcgttggccg cctggacatc gaagaaacct cctgccagcg
    14401 tcgcctcgtc gcacagcggc atcacccgga tgaaacgctc gcccgcgtaa cgccgttcca
    14461 gcgcagcatg cagctcggcc gcgccgacag catctggtaa ttgtgatcga tgtaagggca
    14521 cactgaccgt caaacccttg tagaacgggc ccacgatcgg catgaacacc ggaggaaagg
    14581 cgagaccggt gtgggtcttc atctccggca gatgcttgtg ggccagcgcc agcgcatagg
    14641 gacgcggcgc ggcgagagcg acatacggcg tcgcggcatc gcagcccgct gcggccgaca
    14701 gcggggcagc ctcgtagtcg cggatcatgc tcttgccgcc acccgaatag ccagtgatcg
    14761 aggtcgcggt cagcggtgcg tccgacggaa tcagaccggc atcgaccagc ggccggatca
    14821 acaggatgaa ggcgctggcg tggcagcccg gattcgcgat ccgcttgctc gcgcgaatcg
    14881 ccgcgcgctg ccccggcgcc agttcgggca atccgaaagc ccagcccgca gcggtgcgat
    14941 gcgccgtgct ggcatcgatc agacaggtcg ccggattggt gaccagcgcc gccgcctcgc
    15001 gcgaggcggc atccggcaag cagagaaatg ccacgtcggc cgcgttaagc aagcgcgcgc
    15061 gctcggccgt ctccttgcgc aactcgggat cgatgcgcag cacctcgatg tcttcacgct
    15121 gggcgagata ctcgtggatg cgcaggccgg tggttccctc ctggccatcg acgaacacac
    15181 ggtgcttcat ggtgtttccc ctcaacaaac tggcctgcga ggataagcga ccgcgatgac
    15241 gaccgacatg ggcgcacccc ctggaaacgc cggcggcacc gaccgccgcg tgctcctgct
    15301 tccgggctgg caggattccg gtgcgactca ctggcaaagc cgctgggagg cgctgcatgg
    15361 ataccgccgc gtccagcagg acgattggct gtggccgcgc cggggcgact ggatgactcg
    15421 cctcgaggag acactgctcg agaacccgac cccggccgta ctggtggccc acagcctggg
    15481 ttgccacctg gttgcggcct gggcggctca ctcgcggcat gtcgcgcgag tgcatgcagc
    15541 cctgctggtc gcgccgcccg acgccgaacg cgacgacaca cctgcgaacc tgtacagctg
    15601 gcgaccgatc gtgcgtcagc ggctgccgtt cagatcgctg gtggttgcca gcagcgatga
    15661 tgcgtactgc agcctcgcgc gcgcctccgg tctggccgca gactggggca gcgcgtggtg
    15721 cctgctgggc gcacggggcc acatcaacag cgagtcgcaa ctcggcgagt ggtccgaagg
    15781 acatgccctg ctgcaacctc tgatgagctg atcacggcgc ggagcagggg acaacaccgc
    15841 tgcagcatcc ccggcgcgat gccggacaat ccacagatgg tcaccaagaa acccaagggc
    15901 ctcggcctcg gcctcgaagc actgctcggg ccccgcgtga aggaacccac cgatgcaccg
    15961 gcagccgacg ccgacctcgc gccgcgcagc ctgaagctga cgcaactgcg ccccggcaag
    16021 taccagccgc gaacgcggat ggacgagggc tcgctctatg agctggccga aagcatcaag
    16081 gcgcaaggga tcatgcaacc gatcctcgtg cgccgcgtgc ccgatgcggc gagcggttcg
    16141 tcgaccgatg cgcctcaata cgaaatcatt gccggcgagc gccgcacgcg ggccgccagg
    16201 ctggccggcc tcgacgaagt gccggtgctg gtcaaggacg tgccggacga agccgcggct
    16261 gcgatgtcgc tcatcgagaa catccagcgc gaagacctca atccgctcga ggaagctcag
    16321 ggcctgaagc gactcaccga cgaattcggt ctgacacatg aacaggctgc ccaggccgtg
    16381 ggccgctcgc gcagcgccgc gagcaacctg ctgcgtctgc tgaacctggt cgagccggtg
    16441 cagcagctgc tgatggcggg cgatctggac atgggccatg cccgtgcgtt gctgccgctc
    16501 gacggtgcac gccagatcct ggcggccggc gagatcgtcg ccaggaagct cagcgtgcgc
    16561 gaagccgagc ggctggtcgc acgcgccgac gacagcggcc ggcaaaagcc ggtcaagcgg
    16621 gtggccgatg cgaagtcgcg cgacctgcaa cgcatcgagc aggaactgtc cgatgcgctg
    16681 accgcccggg ttgaagtgcg cgtgaagaag cgcaccgcgc ggggcgagca gggcgagatc
    16741 tcgatcgcct tcggttcgct cgacgagctc aacggactgc tcgacaagct cggcctcccc
    16801 aagcgctgag tcatcaggct cgggcccgga acgcccgacc tttctgaaaa cggaggcgtt
    16861 cggcgcctcg cccgatcgcg cccgccttcg tacggcccga gctcgaatcc gccagacccc
    16921 ggggccgtcg ggccgcacct gccaggaagc gcacgcccct gcacaaccca ctggatccgg
    16981 ccgcccgcgc aagcgcgagc catggcgtgc agtgcccggc gcaggtggcc actctgcgcg
    17041 taaacccgcg ccaccacaca gctgcgctgc gggttcggga cgagatgagc ctgaaatccc
    17101 tcgcaaatcc aggggttcgt tttatttaaa tagagcaaat tagacaaatt ggttgtcgat
    17161 tgcccggagc cccattcagt acccaaaaag tgcgtcgata cgtctggtga cgatgcccca
    17221 gatcaacgtt gcccgaacac cgacccctac ccgcgactgc ctgcagacac cccatgaacg
    17281 cttcgacgat cgcccaaccc ggactgacca gcactccgga cgatggaacc ctcgcgacct
    17341 tcgccaaggc catcatgcgc agcctggcag gccggcgcga caaacccggc acgatttcct
    17401 cggccgagac actttgttct cggcttgacg aggcctcccg cacctggacc acgcacctgg
    17461 gcacggccca ggcgcagatg cggcaggcca ccgagcaact gctgtcgggc tttgcccaga
    17521 tcctggagca gctcgacgcg atcatccaac ccgcccacgg cgcggcctcg ggtcagccgg
    17581 tcacggccag cgggctggac agccgcgccc aggtgctgga agcctgcgag tcgcgcctgc
    17641 gcggcctgat ccagaacttc cagggcttcg tgcagtctcg cgacgcggtc atgcagtcgg
    17701 tgcggcagct cgccggcgag tccgtgagcc tcggcaccat ggccgaggac gtggcccaga
    17761 tcgcacggca gaccaacctg ctttcactca atgcggccat cgaggcggcc cgagccggtc
    17821 ccagcgggcg cggattcgcg gtggtggcca ccgaggtgcg gcgcctgtcc accgagtccg
    17881 gcgacaccgg caagcgcatc ggtgatcacg tccaggcgtt cggccagcac atgaatgcgg
    17941 cgctgaagca ggcctcggag cacaccgcgc acgacgcgca ggtgatccac gcgtcagagc
    18001 agaccatcaa ccaggtcatc gaacaggtcg acagcgccgt ctcgcaactc aatgagcgcg
    18061 ccgccgaact gagcgcacgc ggggaggccg tgcgggcgca ggtcgagcaa ctgatgatcg
    18121 ccttccagtt ccaggaccgg gtccaccaga tcatggacca ggtcaattcc tcgatcagca
    18181 gcgccgtcgc gcggctccag caatcgctga aagacggcgc cgcaccggat gccgatgagt
    18241 ggacggccct gctcagtgcg ggctacacca ccgacgaaca gcgtttcgcc tcggatcacg
    18301 gctcgcagaa cgcggcggcc ccatcgagca acgacaccac cttcttctga cgtttcgcag
    18361 acccggcgtc cttttccgac tcacgagatc ccctgaacca tggccaagac catcctgata
    18421 gtcgacgact catcctccct gcgcaccgtg gtcaggatgg cgctggtacg cgccggctac
    18481 gacgtgatcg aagccggcga tggcaaggaa ggcctggccc agctcgacaa ggcgggcaag
    18541 gtcaacctga tcgtcagcga cgtgaacatg cccaacatgg acggcatcac gttcgtcacg
    18601 catgtcaagg cgcacccgcg ccacaagttc acccccgtgg tcatgctgac caccgaaggc
    18661 caggacgcca agaaagagca gggccgcgcc gccggcgcca aggcctggat cgtcaagccc
    18721 ttcaacccac cgcagctgct ggacgccgtc tccaagctga tcctgccgta ggagcgtgcc
    18781 atgacccaca ccctgtccct gcccgcggaa ctcacgatct acacggtggg cgaactgcgc
    18841 ccccagtggc tgagctggct ggccggcttc gccgccgacg acgcgccggc cgatgcgtcg
    18901 gtggacggct cggcggtcga ccagatcgat gccgctggcg tgcagctgct gatggcgctg
    18961 tcgcgctcgc tggcagccgc acaacgctcg ctgcagttcc tgagcgtcag ccccgtgctc
    19021 gccaccgcct gcgcggcgct gggcctgccg gtttccatgg ccgcgcccga tgaccaggca
    19081 ggtgaagcat gaaacagcag caggatctgg acaacagctt catcgccgag gcgctgcctg
    19141 ccttcatcag cgaagcgcag gaacagctcg agatgctgga gcagctgctg ctccagctcg
    19201 aggatgcgcc ccaggacagc gaactgctcg gcgcactgtt tcgttgcgct cacacggtca
    19261 agggatcggc aggcatcttc gggctcgacg aggtggtggc attcacccac cacgtcgaga
    19321 cgctgctcga ccggctgcgc gaaggccgcg tcgcgctgac gccgcagctc agcacgctgc
    19381 tgctgcagtg caatgaccag atccgtgcgc tgatcgccgc cgcccaggac ccgcaggccg
    19441 acgacgccag cgcagctgaa gctcgtgcgg cgctcgtgct gcgcctgcag gcagccagcg
    19501 gtgacggcgc gcccgatgcg gctgctccgg aggaagccag cgcaccggct gcgacgctcg
    19561 ctgcgggcgt gcgccgatgg cacctgtggg tgaagttcgg cgaggacacc tatcgcaacg
    19621 gcatggaccc gctggccatc ctcacctacg tgcgcgggct cggctcgatc ccgagcctgg
    19681 tctgcgacct ggctgacgtg cccacgctcg agtcgatcga ccccgagaac tgccacctcg
    19741 gcttcgagtt cagcctcgag accgaggcct cgcgcccgga gatcgaggcc gcgttcagct
    19801 tcgtgcgcga ggactgcacg ctgcacgtga ccgaaccgaa cgcgaccgcg aaggatttcg
    19861 ctcgcctgat cgagtcgatg cccgacaacc agcgtctcgg cgacatcttg gtgtccgtcg
    19921 gtgcgatcag ccgcgtgcag ctcgagcagg cactcaggga gcaggccgcc gcgtccgcca
    19981 acggcaacgc gcaggcgctc ggagagatcc tgcaggccaa gagcggcctg agcccgaaac
    20041 tcgtcgacgc cgcgctgcag aagcagaaca agcagcgcga gggcggcagc accgcgccgg
    20101 cggccagcga cgacaaccgc ttcattcgcg tgcaggccga tcgcctcgac gccgtcatca
    20161 acttgctcgg cgagctggtg atcgccggtg ccggcgcctc gctgctggcg cgccagaccc
    20221 gccagggcgc gctgatcgag gccactgccc agatcggcag cctcgtcgag gaaatccgaa
    20281 acggcacgct gcagctgcgc atggtgccca tcggcgagac cttctcgcgc tttcgccgcg
    20341 tcgtgcgcga caccgcagcc gagctcggca aggacgtcgc gctggagatc gtcggcggcg
    20401 agaccgaact cgacaaatcg gtggtcgagc gcatcgccga cccgctgatg catctggtgc
    20461 gcaacgcgct cgaccacgga ctggaaacgc ccgagcagcg cgccgaggcc ggcaagcccg
    20521 cccagggcag gctcacgctg agcgcctgcc acgaatcggg cagcatcctg atccgcatcg
    20581 tcgacgacgg ccgcggcatc cagcgcgaaa gggtgctgca gcgcgcctgg gaccgcggac
    20641 tcgtcgagca gggcgtgacg ccgcccgagg ccgacatcct caagctgatc ttcgagcccg
    20701 gcttctccac cgccgagaag gtgaccaacc tcagcggtcg tggcgtgggc atggatgtgg
    20761 tgcgcagcaa catccaggcg ctgcgcggca gcgtgacgct caccagcacc gaaggccagg
    20821 gctcgacgat cgagatccgc ctgccgctga cgctggccat catcgacggc ttcctcatcg
    20881 gcgtgggcaa ctccaagttc atcttcccgc tggacgcggt ggtggaagtc atcgccaacc
    20941 gcgcccgccc cgagcagctg gacgagcgcg gccgtggcgt ggtggagctg cgcggccagg
    21001 tgctgccggt ggtgagcctg cgcgcgctgt acgcgctgga aggcgacgaa cccgcacgcg
    21061 gcagcgtggt cgtgctgcag gccggtagcc gccgctacgg cgtgctggtg gaccagctgc
    21121 tgggccagca ccagaccgtg atcaagccgc tgggccgcat gttccgcagc ctgcgtggca
    21181 tgtccggctc atcgatcctc ggcaacggcg aagtggcgct catcttcgac gtgca
//
LOCUS       DAOORV010000058        20959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3093_length_20959_cov_8.836586, whole genome shotgun sequence.
ACCESSION   DAOORV010000058 DAOORV010000000
VERSION     DAOORV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3093_length_20959_cov_8.836586"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="ligA"
                     /locus_tag="PKV56_09495"
     CDS             complement(<1..1028)
                     /gene="ligA"
                     /locus_tag="PKV56_09495"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOX68085.1"
                     /translation="MIEDDLERRAAELSALLKQHAHSYYVLDAPSIPDAEYDRLYQEL
                     EAIEAANPALRTDDSPTQRVIGGVLEGFSAVRHAVPMLSIRTETDTEASGAIAFDARV
                     RRELGLAESDPPVEYAAELKFDGLAMNLRYESGVLVQAATRGDGETGEDVTQNIRTIA
                     QIPRRLQLGNLCAPPEVLEVRGEVYIRRDDFEALNERQRALIAQGDKREKTFVNPRNA
                     AAGAVRQLDPAIAARRPLSFYAYGLGEVRGWGADNSALPATHAALLDALAAMGMPVCA
                     ERAVVQGAAGLVDFHASMGARRDLLPFDIDGVVYKVNDRALQHRLGFVTREPRWAVAH
                     KYPAQEQMT"
     gene            complement(1066..2184)
                     /locus_tag="PKV56_09500"
     CDS             complement(1066..2184)
                     /locus_tag="PKV56_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOX68086.1"
                     /translation="MSGDLTLALAALGGVVLVGVVAHGAWQARRADPKKAVTADSRFE
                     PREPVMGGAEDAAPGDALPAGSRSDARSPGASAASPGRDELTIDGAPSRQQLRRSMPR
                     VDALIDVIVPMTLEAPVTGDAVLSHLPGSRRAGSKPFFIEGLNADNGEWEAPVAGQRY
                     GDLQAGLQLANRSGALNEIEYSEFVQKLEVFADAVGAMADFPDMLDVAARARELDAFA
                     GEHDAQMSVQLRARSAAWSVGYVVQQASRHGFVHGVLPGRLVLPSAEEGAPPVLTLTF
                     DSQAALSDDPGAAALRELALGFDVSQTDAAASPFAAWHASAIALSQDLDADVVDDRGQ
                     PLTSAGFTFIDGELVRLYEALQARDLAAGSPAARRLFS"
     gene            complement(2219..5740)
                     /gene="smc"
                     /locus_tag="PKV56_09505"
     CDS             complement(2219..5740)
                     /gene="smc"
                     /locus_tag="PKV56_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOX68087.1"
                     /translation="MRLNSIKLSGFKSFADPTHFQLPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMQDVIFNGSGQRKPASRASVELVFSNEDARAGGQWNQFAEIA
                     VKRVLTRDGSSSYHINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESKPEEL
                     RLFLEEAAGVSKYKERRRETENRLRDTRDNLTRVDDILRELNGNLEKLEKQAEVAGRY
                     RSLQDDGTLKLHQLWFLRHRDAAGEQQRIEKEVSTAVNALEARVAELRQGEAELETIR
                     QAHYAASDELHASQGRLAEASLEVSRLEERIRYVVEGRQRAEQRLAELSLQTAQWADR
                     RAQAEAELEQLAEQMVSADEHSEVLAAQAEEQADRLPGLEEAVRGAQSAASTQRGVVA
                     GVQQQIQVLASDSRNLEDQNRQLVARREKLAGEKRQLAAPDLDRIEHLKQQFSSAEES
                     REVAEARLADLGEQVPALDETRRLRQSEVNAEAARQSDFSARLDALRALQDKVQTEGK
                     LRPWLARHGLDGLSGLWTRIHIEPGWETALEAALRERINALEVGRVETVRAFADDAPP
                     AKLVFYTPPVAGTPQATSNATLSRLSDLLRVGDAGLKALLTDWLEGVYTAASLDDALA
                     ARSRLQHGEVLMTREGHAASAHAVSFYAPDSEQAGLLARAQEIENLDRQLRAQSLIAE
                     EARSALIRAEAAYTGASAHLATARRDSAESQGRAHQLQVELLRLTQQAEQTQSRSTQL
                     DDELAEIDAQLEELNERRATGEARFEELDIELAATQERHAELDDAVIQAERVLAAARE
                     QLRTLERQAQESQFAARSLAARRSELQRGIETAIQQVASNAQGQEQLNEELVRLTDAT
                     AQVGLQEALALKLEREAALGAQRSAYDDLTLRLRRADEQRMVHEQSLQPLRDRITKLQ
                     LEEQAAQLGGAQYLDQLVAAAVDFESLSQGIERDGVRLQGLQGEIDRINREITALGAV
                     NLAALDELTTARERKTFLDAQNADLMDAIGTLEGAIHKIDLETRDLLGSTFNQVNEHF
                     GRMFPSLFGGGQARLVMTGDEILDAGVQVMAQPPGKKNSTIHLLSGGEKALTAIALVF
                     AIFQLNPAPFCLLDEVDAPLDDANTERYAKLVKEMSKSTQFLFISHNKIAMEMAVQLI
                     GVTMQEQGVSRIVAVDMAEAVNLAEAA"
     gene            5889..7220
                     /locus_tag="PKV56_09510"
     CDS             5889..7220
                     /locus_tag="PKV56_09510"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX68088.1"
                     /translation="MKTWLNRLLGSEPARKTPQKSAPVTLPASTAKPAEAGAGFAVRR
                     PLVAANGNVAGFEFCLPASVEERLRRKADPIAQGAHALALMSSMRSTLDNGRVALATI
                     PLAILARPNVVDHAPAGAHLCIDGAADDDAGSPGSVDEAALLASLRARDVRVGALLRQ
                     PTDAAKLDFAVLSATTDGLESMLAAARQWREANGSRTLIATQLASVDEIELALAAGAT
                     LACGSFNASAAAPPQRALQGDVQRIIQLLNSVRSEEKPLADLTQDLRADVALCYRLLR
                     HVNSPAVGLSRPVESIDQAVLLLGRSELYRWLSVLLLSSVNGRRASRALQEISLARAR
                     LAESLARERSGDAPDALFTVGLLSLLDVMLQVPLTQALGPLRLTDPARQALIERGGPW
                     RDILALAEALERHDLTSAGELADAFGGLDRVLTLSDEAWGWAASVQRELDA"
     gene            7285..8496
                     /gene="dapC"
                     /locus_tag="PKV56_09515"
     CDS             7285..8496
                     /gene="dapC"
                     /locus_tag="PKV56_09515"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOX68089.1"
                     /translation="MNPLLARLQPYPFERLRALTRDITPNPALAPISLGIGEPKHPTP
                     ALVTQAITDNLAGLAGYPATAGEPALREAIVQWIGRRYGVTVDAGSQVLPVNGSREAL
                     FALAQTVIDPTRGDATVVCPNPFYQIYEGAALLAGARVEFVNSDPARQFAADWDAVPE
                     ATWARTQLLYVCSPGNPTGAVMPLAEWRRLFELSDRHGFVIASDECYSEIYFRDEPPL
                     GSLEAAQQLGRNDFRRLVAFTSLSKRSNVPGMRSGFVAGDAEILKSFLLYRTYHGSAM
                     SPTVQRASVVAWNDEAHVVANREQYRRKFAQVTPLLAEVMDVALPDAGFYLWADISRL
                     IPGGDDVEYARGLLAQYNVAVLPGSLLAREAGGVNPGAGRIRLALVSEADSCLEAAHR
                     IVAHCRSMISA"
     gene            8571..9392
                     /gene="dapD"
                     /locus_tag="PKV56_09520"
     CDS             8571..9392
                     /gene="dapD"
                     /locus_tag="PKV56_09520"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOX68090.1"
                     /translation="MTANLQSIIEAAWDNRANLTTTSHPEVNAAVETVISDLNAGRVR
                     VADRQGVGQWSVNQWVKKAVLLSFRLNDNELMHAGDLTFFDKVKTKFSHIDESAVRAS
                     GVRIVPPAVARRGSFLAKNVVLMPSFVNIGAYVDENTMVDAWATVGSCAQIGKNVHLS
                     GGVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEENSVISMGVYIGQSTKIYDR
                     ATGEVSYGRVPAGSVVVSGNLPSADGTHSLYCAVIVKKVDAQTRAKTSLNDLLRA"
     gene            9463..10650
                     /locus_tag="PKV56_09525"
     CDS             9463..10650
                     /locus_tag="PKV56_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOX68091.1"
                     /translation="MSGNMERVLRLMSEKSASDVYLSANTPILIKINGQIMQLSDQPL
                     TPAQPRQLLAEVLTPKQLEELDDTGELNVGVGLPGVGSFRLSGFKQRGSIAAVFRCIP
                     VDIPTLESLQVPSLLNTLVMEKRGLILMVGSTGAGKSTTLAAMLEQRNQQMAGHILTI
                     EDPIEFLFNNKKSVVNQREVGRDTQSLQVGLRNALRQAPDCILIGEIRDRETMTAAIS
                     YALSGHLVLSTLHANNSYHALGRILSFYTPESRPALLSDLASGLRAIVSQRLVRATAG
                     GRIPAVEVMLNTKLVSDLITAGDFGGVREAMEKSLAEGSQTFEQDLARLITDGTISRD
                     EGLAFADSPTNLMWRLQNDMSPVSRITAKKEEVDEGASFTEITLDVRPEPSNTRAAPL
                     SRM"
     gene            10708..11613
                     /gene="prmB"
                     /locus_tag="PKV56_09530"
     CDS             10708..11613
                     /gene="prmB"
                     /locus_tag="PKV56_09530"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOX68092.1"
                     /translation="MTLLDLIKREAARLKKAGVSFGHGTANAFDEAAWLVLWSLGMDL
                     DALETHAKREASADEEAKAVALVTRRIETRRPAAYLTQEAWLQNVPFYVDERVIVPRS
                     FIAELLVDGEGQGTLDAWLSDRTQHVLDLCTGNGSLAVIAALAYPEIEVDACDLSDDA
                     LAVAKINVERHKLSKRITLMKSDLLDSVRGPYDLILCNPPYVNSSSMAALPDEYRHEP
                     ALALAGGEDGMDLVRRIVNDAVAKLTDIGVLVLEIGNERGHFEAAFRRLEVAWLETSA
                     GDDAVLLVTREALLRWSKRSETWRA"
     gene            11618..13624
                     /locus_tag="PKV56_09535"
     CDS             11618..13624
                     /locus_tag="PKV56_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX68093.1"
                     /translation="MITLKDVTLRRGTKVVLDAASVTLQPGENVGLVGRNGAGKSSLF
                     AMLTNRLQSDGGEVYMPPRWRVGEVAQNMPETDDGATDFVLEGDTRLVEAQAQLAAAE
                     AGDDGHAIADGHQALADAGAFDARPRAQALLMGLGFKNEQLDAPVNSFSGGWRMRLQL
                     ARALMCPADLLLLDEPTNHLDLDALVWLEAWLKRFEGTMLVISHDREFLDAITHVTVH
                     LEDAKLTRYGGNYTAFEEMRAERMVLQQASFEKQKEKMAHLQKFIDRFKAKASKAKQA
                     QSRVKALDRMEKLAPILTSREFSFEFREPLNLPNPMLSLKDVECGYHALEEGGPDKII
                     VRNINRSVLAGQRIGILGANGQGKSTVVKTIARAQAALGGTITEGKGLAIGYFAQQEM
                     DVLRPGDSPLNHMIRLAREVSPQAREQELRNFLGQFRFAADMVNQPVGTLSGGEKARL
                     VLAMLVWQRPNLLLLDEPTNHLDLDTREALSMALNEFEGSLMLVSHDRALLREVCDEF
                     WLVADGGVRPFDGDLDDYQKYLLDTARAQAKALREAGNASKKAAAPAPASAPVAPAPK
                     PSTAATAPAKREDRKADAQARQLKSDELKPLRKELNRIDNRLGALFGDRDALEAGFAT
                     GSMSPAQLAESGKKLKAITEEIGLLEGQWLDLSTQVDELTAKAG"
     gene            complement(13672..14691)
                     /locus_tag="PKV56_09540"
     CDS             complement(13672..14691)
                     /locus_tag="PKV56_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOX68094.1"
                     /translation="MHPRIKPAHPTHPVGGAGTVVVLAMGGTIAGTADSPGDHLGYKS
                     AQLGIVQLLAGLPELAGLPIESEQVAQIDSKDMTHAIWQSLAQRVAHHLARAEVLGVV
                     VTHGTDTLEETAYFLQRVLAPAKPVVLTGAMRPATSSEADGPRNLLDAVTLARDPGAT
                     GVLAVLSGTVHGARNVRKVHPTRLDAFSSGEAGLLGAIEDGRFVAAADRTAAFAAMDS
                     TAPVAAALTHPVAQWPRVEIVLSHAGAGAAAVRALCGDGTEGTRCVDGLVVAGTGNGR
                     VHHDLEAALLEARSRGIAVLRSLRCGDGRLVPVAGDRLPGAEDLSPVQARIELMLALM
                     EHQPT"
     gene            14852..16030
                     /locus_tag="PKV56_09545"
     CDS             14852..16030
                     /locus_tag="PKV56_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68095.1"
                     /translation="MNTSSIATTQALASQTGYSLSIRASSGPARPEGTDDSSEVRRLR
                     HGDHEHHHDHDHRAPRSDDQGYSGSAFTSLKLKIEETFSSLTGRTGTDAEDSTSSSEF
                     EGEFQARIQFSGPDGEFKAKLKFSLEGDSATAGADFASAMQGFAQTLFAALNALYGGG
                     STPALPGPTESTAPVGTDPAATPALPAPPPAPAPVADETVAAAPAPTANPEPVVVAPP
                     AAPSTPTTGSFSPTTTSFSIKLRATYDSFENNLGPLVNQLAQPDVGDAFPALTSLLRD
                     LADRFGQLVSQDPASATSAPTLQDFLKALSGSFFNPQPEASAEADAADPSTPALPTAE
                     TGAQPTDPVVADTPTAPAPTSQRFTAMAQYKQVLTYSDAGSSLSLQTSMSARLVYEVA
                     "
     gene            16173..16850
                     /gene="lexA"
                     /locus_tag="PKV56_09550"
     CDS             16173..16850
                     /gene="lexA"
                     /locus_tag="PKV56_09550"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOX68096.1"
                     /translation="MQDAPKLTDRQQQILDLVQSAIERTGAPPTRAEIAAELGFKSAN
                     AAEEHLQALARKGVIELVGGTSRGIRLKSETLRALNEMRGKQFSLPLPSLSQLTLPLI
                     GRVAAGSPILAQEHIDQTYLMEASMFPRRPDYLLKVRGMSMRDAGIMDGDLLAVQKAR
                     DAKNGQIVVARLGDEVTVKRFKRTRTTIELHPENPDFEPIVIPFGDVDFELEGLAVGL
                     IRNNMMM"
     gene            16973..17479
                     /locus_tag="PKV56_09555"
     CDS             16973..17479
                     /locus_tag="PKV56_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68097.1"
                     /translation="MLNRWTHRLQSTWAKRGRPASDRQEAQRPLQPATARHVEICPPD
                     RLYRRSRWRRGLRDWLNTGWDVSSPAALTVERTPATPASEAEPTPFADIRQEFANALN
                     DIDTDAAAQLRAQIERARSPRELWHLRSALFTMVARHHSESQAQSRMERLNRHFPNRS
                     PASGPGTR"
     gene            17481..17678
                     /locus_tag="PKV56_09560"
     CDS             17481..17678
                     /locus_tag="PKV56_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68098.1"
                     /translation="MPAHRHGPQPDHELWRAVHERPAAPTPWPFDRGSPAIALHGMPA
                     DRLAMTASLRSIQLQAHHRSR"
     gene            complement(17689..18198)
                     /locus_tag="PKV56_09565"
     CDS             complement(17689..18198)
                     /locus_tag="PKV56_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HOX68099.1"
                     /translation="MSISMYSASVPVFEKMLGNLLRWLDAAEAHAVAKKFDTSVYLAA
                     RLAPDMLPFTKQIQIACDMAKYGVARLSGDSAPKFDDNETTMAELRQRIAKTLDYIKS
                     VAADQIDGTEAREIEVPIRGASPLKFAGEAYLKHWVLPNFYFHVTIAYALLRHNGVEI
                     GKFDFIGRD"
     gene            18310..19317
                     /locus_tag="PKV56_09570"
     CDS             18310..19317
                     /locus_tag="PKV56_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HOX68100.1"
                     /translation="MNIIILDDYQDAVRKLKCAEKMEALHAKVFTNTVKGIGQLSIRL
                     RDAEVLVLIRERTHFPRQLLEKLPRLRLISQTGKVGSHIDVEACTRLGIAVAEGVGSP
                     VAPAELTWALIMAAMRRVPQYIGNLKHGAWQQSGLKASSMPANFGIGMVLNGKTLGVW
                     GYGRIGQLVAGYARCFGMRVMVWGSESARERAELDGHASAHSCESFFEECDVLSLHLR
                     LSETTRGVVKIEHLSRMKPTSLLVNTSRAELIEEGALVSALNRGRPGMAAVDVFESEP
                     ILQGHPLLRLENAVCTPHIGYVEQDSYEEYFGAAFDNVVSFVEGRPTHIINPEALKVQ
                     R"
     gene            19314..19892
                     /locus_tag="PKV56_09575"
     CDS             19314..19892
                     /locus_tag="PKV56_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOX68101.1"
                     /translation="MSASSSETELAAPRSNADLFLTFTWLALQGFGGVLAVAQRELVE
                     RKRWLTREQFVEYLSVGQVLPGPNVVNLSMMIGDRFFGLRGAMSALAGMLAMPLVIVL
                     LLASLYGRYESQPAVQGALRGMGAVAAGLVLATALKLLPTLRSSALGVPAGLGFAAVA
                     ALSVTAWHWPLWWVVTGIGSLAVALAWWRLRP"
     gene            19963..20490
                     /locus_tag="PKV56_09580"
     CDS             19963..20490
                     /locus_tag="PKV56_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOX68102.1"
                     /translation="MTPVDLPALFGHFLLLSLLAVGGAITTAPDMHRYLVTEHGWISD
                     AQFTTSVALAQAAPGPNLLFVAVLGWNVAGPMGAVATMTGILLPSTLLTLWATRWGQA
                     RRDTRGVRAFTTGMAPLTLGLLVATGWVLTEPVRMQVGAWALIAITVVVMVRTRVSPM
                     WLVALGAVVGAMGYA"
BASE COUNT         3428 a         7220 c         6988 g         3323 t
ORIGIN      
        1 gtcatctgct cctgggccgg gtacttgtgc gccaccgccc agcgcggctc gcgcgtgacg
       61 aatcccagcc tgtgctgcaa cgcgcggtcg ttgaccttgt agaccacgcc gtcgatgtcg
      121 aacggcagca agtcgcgcct ggcccccatg gacgcgtgaa agtcgaccag accggctgcg
      181 ccctgcacca ccgcgcgctc cgcgcacacc ggcatgccca tcgccgccag cgcatcgagc
      241 aacgcagcgt gcgtggccgg cagggcgctg ttgtcggcgc cccaaccgcg gacctcgccc
      301 aggccgtagg cgtagaaact cagcggtcgc cgcgccgcga tcgccggatc gagctggcgc
      361 accgcgccgg ctgccgcatt gcgcgggttc acgaaggtct tctcgcgctt gtccccctgc
      421 gcgatcagcg cacgctggcg ctcgttcagt gcctcgaaat cgtcccggcg gatgtacacc
      481 tcgccgcgga cttcgagcac ctcgggtggg gcgcacagat tgccgagttg caggcggcga
      541 gggatctggg cgatcgtgcg gatgttctgc gtcacgtcct cgccggtctc gccgtcgcca
      601 cgagtcgcgg cctgcaccag cacgcctgac tcgtagcgca gattcatcgc caggccgtcg
      661 aacttcagct cggcggcgta ctcgaccggc ggatcggatt cggccaggcc gagctcgcgc
      721 cgcacacgcg catcgaaggc gatcgcgcca ctggcttcgg tatcggtctc ggtacggatc
      781 gacagcatcg gcaccgcatg ccgcaccgca ctgaatccct cgagcacacc gccgatgacc
      841 ctctgcgtcg gcgaatcgtc ggtgcgcagt gcggggttcg cggcctcgat cgcctcgagc
      901 tcctggtaga gccggtcgta ctccgcatcc gggatcgacg gcgcatcgag cacgtagtag
      961 ctgtgcgcat gctgcttcaa cagcgcgctc agttcggccg cgcggcgttc gagatcgtct
     1021 tcgatcatcg agcaggctcc cggcgccgcg ctgacgcgca gcagatcaac tgaacaggcg
     1081 ccgcgcagcc ggcgagcctg cggccagatc gcgagcctgc agtgcttcgt agaggcggac
     1141 gagttcacca tcgatgaagg tgaatccggc agaagtcagc ggctgcccgc ggtcatcgac
     1201 gacgtcggca tcgaggtcct gcgacagggc gatcgcactc gcgtgccagg ctgcgaacgg
     1261 agacgcggcc gcgtcggtct gcgacacgtc gaagcccagc gccagctcgc gcagggccgc
     1321 tgcacccgga tcgtcggaca gcgcggcctg cgaatcgaaa gtgagtgtca gcaccggggg
     1381 cgccccctcc tcggccgacg gcagcacgag ccggcccggc agcacaccgt ggacgaatcc
     1441 gtgccgcgag gcctgctgca cgacgtagcc gacgctccac gctgcgctgc gagcgcgcag
     1501 ctgcacggac atctgcgcat cgtgctcgcc ggcgaacgca tcgagctcgc gggcgcgtgc
     1561 ggcaacgtcg agcatgtccg ggaaatcagc catcgcaccc acggcatcgg caaacacctc
     1621 gagcttctgc acgaactcgg aatactcgat ctcgttgagc gcgccgctgc ggttggccag
     1681 ctgcaggccc gcctgcaaat cgccgtagcg ctgcccggca acgggagcct cccactcgcc
     1741 gttgtcggcg ttcaggccct cgatgaagaa cggcttgctg ccggcccgtc ggctgccagg
     1801 caaatgcgag agcaccgcgt cgccggtgac cggcgcctcg agcgtcatcg gcacgatcac
     1861 gtcgatcagc gcatcgacgc gcggcattga gcgacgcagc tgctggcgac tcggcgcccc
     1921 gtcgatcgtc aattcgtccc ggcccggcga cgcagcgctg gcgccagggg atcgtgcgtc
     1981 gctgcgcgac cctgcgggca gcgcgtcgcc cggcgcggca tcttcggcgc cgcccatcac
     2041 cggctcgcgc ggttcgaagc gcgagtcggc agtcacggcc ttcttcgggt cggccctgcg
     2101 tgcctgccag gcaccgtggg ccaccacgcc caccagcacg acaccgccga gtgcggccag
     2161 cgcgagggtc aggtcaccgc tcatgaacgc gccttgctcg cgcagacgac ggcaccgctc
     2221 atgccgcctc cgccaggttc acggcttccg ccatgtcgac cgcgacgatg cgcgaaacgc
     2281 cctgctcctg catcgtcacg ccgatcagtt gcaccgccat ttccatcgcg atcttgttgt
     2341 gactgatgaa caggaactgc gtgctcttcg acatctcctt gaccagcttg gcatagcgct
     2401 cggtgttggc gtcgtccagc ggcgcatcga cttcgtcgag cagacagaac ggcgcgggat
     2461 tcaactggaa gatcgcgaac accagcgcga tcgccgtcag cgccttctcg ccacccgaca
     2521 gcaggtggat cgtgctgttc ttcttgcccg gtggctgcgc catcacctgg acgccagcgt
     2581 cgaggatctc gtcgccggtc atcaccagcc gggcctgacc gccgccgaac aggctgggga
     2641 acatgcggcc gaagtgctcg ttgacctggt tgaaggtgct gccgagcagg tcgcgggtct
     2701 cgaggtcgat cttgtggatg gcaccctcga gcgttccgat cgcatccatc aggtcggcgt
     2761 tctgcgcgtc gaggaacgtc ttgcgctcgc gcgcggtcgt cagttcgtcg agtgcagcca
     2821 gattgactgc gccgagggcc gtgatctcgc gattgatgcg gtcgatctcg ccctgcagtc
     2881 cctgcagccg aacgccgtcg cgctcgatgc cctgcgagag cgactcgaag tcgacggccg
     2941 cggccaccag ctgatcgagg tactgcgcac cgccgagctg cgcggcctgc tcctcgagct
     3001 gcagcttggt aatgcggtcg cgcagtggct gcaggctctg ttcatggacc atgcgctgct
     3061 cgtcggcgcg gcgaagtcgc agggtcaggt cgtcgtaggc gctgcgctgc gcgcccagcg
     3121 ccgcctcgcg ttcgagcttc agcgccagcg cttcctgcag accgacctgg gccgtggcgt
     3181 cggtgagccg caccagttcc tcgttgagct gctcctgccc ctgcgcgttg ctggcgacct
     3241 gctgaatcgc ggtctcgatg cctcgctgca gttcgctgcg gcgcgccgcc agagaccgcg
     3301 ccgcgaactg cgactcctgg gcctggcgct ccagcgtgcg caactgctcg cgcgcggccg
     3361 ccagcacccg ctcggcctgg atcaccgcgt cgtcgagctc ggcatggcgc tcctgggtcg
     3421 ccgccagttc gatgtcgagt tcctcgaagc gcgcttcgcc cgtggcgcgg cgctcgttga
     3481 gctcctcgag ttgggcgtcg atctcggcca gttcgtcatc cagctgcgtc gagcgcgact
     3541 gcgtctgctc ggcctgctgc gtcaaccgca gcagttcgac ctgcagctgg tgcgcgcggc
     3601 cctgcgactc ggccgagtcg cggcgcgccg tggccaggtg cgcggaggcg ccggtgtaag
     3661 ccgcttcggc ccggatcagc gcgctgcgcg cttcctcggc gatgagcgac tgcgcgcgca
     3721 gctggcggtc gaggttctcg atctcctgcg cgcgtgcgag caggccggcc tgctcggagt
     3781 cgggcgcata gaacgagacc gcgtgcgcgc tggccgcgtg gccttcgcgc gtcatcagca
     3841 cctcgccgtg ctgcaggcgt gaacgtgcgg ccagcgcatc gtcgaggctg gccgcggtgt
     3901 agacaccttc gagccaatcg gtcagcaacg ccttcaggcc ggcgtcgccg actcgcagca
     3961 ggtcactgag ccgcgagagc gtcgcgttcg atgtggcctg cggcgtgcca gccaccggcg
     4021 gcgtgtagaa gaccagcttc gccggcggcg catcgtctgc aaaggcacgc accgtctcga
     4081 cccggccgac ctcgagcgcg ttgatgcgct cgcgcagcgc cgcctcgagc gcggtttccc
     4141 accccggctc gatgtggata cgtgtccaca gaccgctcaa cccatcgagc ccgtgtctcg
     4201 ccagccaggg ccgcaacttg ccttcggtct gcaccttgtc ctgcaaggca cgcagcgcat
     4261 cgagccgggc gctgaaatcc gactgcctgg cggcttcagc attgacctcg gactggcgca
     4321 ggcgccgcgt ttcatcgagc gccggcacct gctcgcccag atcggccagc cgcgcttcgg
     4381 cgacctcgcg ggactcctct gcgctggaga actgctgctt caggtgctcg atgcgatcca
     4441 ggtcgggtgc cgcgagctgt cgtttttcgc cagccagctt ctcgcggcgg gcgacgagct
     4501 ggcggttctg atcctcgaga ttgcggctgt cgctggccag cacctggatc tgctgctgga
     4561 cgccagcgac gacgccgcgc tgcgtgctcg ccgccgactg cgcgccacgc accgcctcct
     4621 cgagaccggg aagccggtcg gcctgctcct cggcctgtgc ggccaggacc tcgctgtgct
     4681 cgtcggcgct gaccatctgc tcggccagtt gctcgagctc ggcctcggcc tgggcacgac
     4741 ggtcggccca ttgcgcagtc tgcagcgaca gttcggcgag ccgctgctcg gcgcgctgcc
     4801 gaccttcgac cacatagcgg atgcgctcct cgagccgact gacttcgagc gaggcctcgg
     4861 ccaggcgccc ctgcgatgcg tgcagctcgt cgctggcggc gtagtgcgcc tggcggatgg
     4921 tctcgagctc ggcctcgccc tggcgcagtt cggccacgcg ggcctcgagc gcgttgactg
     4981 cggtggagac ttccttctcg atgcgctgct gctcgccggc cgcatcccgg tggcgcagga
     5041 accacaactg atgcagcttc agggtgccgt cgtcctgcag gctgcggtag cgcccggcga
     5101 cctcggcctg cttctcgagc ttctcgaggt tgccgttgag ctcgcgcagg atgtcgtcga
     5161 cgcgggtgag gttgtcgcgg gtgtcgcgca accggttctc ggtctcgcgg cggcgctcct
     5221 tgtacttgga cacgccagcc gcctcttcca ggaacaggcg cagttcctcg ggcttgcttt
     5281 cgatgatccg gctgatcgtc ccctggccga tgatcgcgta ggcacgcggg ccgagcccgg
     5341 tgccgaggaa cacgtcctgc acgtcgcgcc gacgcaccgg ctggttgttg atgtggtagc
     5401 tgctgctgcc gtcgcgggtc agaacgcgct tgacggcgat ctcggcgaac tggttccact
     5461 ggccaccggc acgggcgtcc tcattgctga acacgagctc gacgctggca cgactggccg
     5521 gcttgcgctg gcccgatccg ttgaagatca cgtcctgcat cgattcgccg cgcagctcgg
     5581 acgccttgct ttcaccgagc acccagcgca ccgcgtccat gatgttcgac ttgccgcagc
     5641 cgttcgggcc gaccacgccg accaactggc cgggcaactg aaagtgggtg gggtcggcga
     5701 acgacttgaa gccggacagc ttgatggaat tcagacgcat gtacgagagc ggattccggt
     5761 gcgaaccgct atcggcatgc gcacataagt cgttgattta tttgagaaaa catcgaatta
     5821 atcgagctct ccacggcctg cgatgatagc atcgagaccg tgaaaaacga ccgcaaggcg
     5881 gcctggacat gaagacgtgg ctgaatcgct tgctgggctc tgaaccggcc cgcaagaccc
     5941 cccagaaatc agcgccggtg acgctgccgg ccagcacggc gaagccggct gaagcaggcg
     6001 cgggcttcgc cgtgcggcgc cccctggttg ccgccaacgg caacgtcgcc gggttcgagt
     6061 tctgcctgcc cgcctccgtg gaggagcgcc tgcgccgcaa ggccgacccg atcgcccagg
     6121 gcgcgcatgc gctggcgttg atgtcatcga tgcgctccac cctggacaac ggccgcgttg
     6181 cgctcgcaac catcccgctg gcgatcctgg cgcgtccgaa cgtcgtcgac catgcgcccg
     6241 ccggtgcgca tctgtgcatc gacggcgccg ccgatgacga tgccggttct ccggggtccg
     6301 tcgacgaggc agcgctgctc gcttcgctgc gggcgcgcga cgtccgagtc ggtgccctgc
     6361 tgcgccagcc caccgacgcg gcaaagctcg atttcgcggt gctgagcgca accaccgacg
     6421 gactcgaatc catgctggct gccgccaggc agtggcggga agccaacggc tcccgaaccc
     6481 tgatcgccac ccagctcgcc agcgtcgacg agatcgagct cgcgctcgcc gcgggcgcca
     6541 cgctggcgtg cgggagcttc aatgcgtccg ccgcagcgcc gccgcagcgt gcactgcaag
     6601 gcgatgtgca gcggatcatc cagttgctga attcggtgcg cagcgaggag aagcccctgg
     6661 ccgacctgac gcaggacctg cgcgccgacg tggccctgtg ctaccggctg ctgcgccatg
     6721 tgaacagccc ggcggtcggg ctgtcgcgcc ctgtcgaatc gatcgaccag gcggtgctgc
     6781 tgctcggccg atccgagctc taccggtggc tgtcggtgct gctgctgtcg tcggtgaacg
     6841 gccgccgggc gtctcgtgcg ctgcaggaaa tctcgctggc acgggcgcgg ctggccgagt
     6901 cgctggctcg cgagcgcagc ggcgatgcac ccgatgcgct gttcaccgtg ggcctgctgt
     6961 cgctgctgga tgtgatgctg caggtgccgc tgacccaggc cctcggcccg ctgcgcctga
     7021 ccgacccggc gcgacaggcg ctgatcgagc gcggcggccc gtggcgcgac atcctggcgc
     7081 tggccgaagc gctcgagcgc cacgacctga cctcagccgg cgagctggcc gatgcgttcg
     7141 gcggtctcga ccgcgtgctg acgctgtcgg acgaggcgtg gggctgggcc gcttcggtgc
     7201 agcgcgagct cgacgcctga ggtacggccg ggttcctggc aaggctgagc cgccctcggc
     7261 ggccatggca tcgataattt ggcgatgaat cccttgcttg cccggctgca gccctacccg
     7321 ttcgagcgcc tgcgcgcgct gacccgcgac atcacaccga accccgcgct ggcgccgatc
     7381 agtctcggca tcggcgaacc gaagcacccg acgccggcgc tggtgaccca ggcgatcacc
     7441 gacaacctgg caggactcgc gggctatccc gccaccgccg gcgagcccgc gctgagggag
     7501 gccatcgtgc aatggatcgg gcgccgctac ggtgtcacgg tggacgcagg cagccaggtg
     7561 ctgccggtca acggatcgcg cgaggcgttg ttcgcgctgg cacagacggt catcgatccg
     7621 acccggggcg acgcgaccgt ggtctgcccg aatccgttct atcagatcta cgaaggcgcg
     7681 gcgctgctgg ccggagcgcg cgtcgagttc gtcaacagcg acccggcacg ccagttcgcc
     7741 gccgactggg acgccgtgcc cgaagccacc tgggcgcgaa cgcaactgct ctacgtctgt
     7801 tcgcccggca accccaccgg cgcggtgatg ccgctggccg aatggcgcag gctgttcgaa
     7861 ctgagcgacc gccacggatt cgtgatcgcc tcggacgagt gctattcgga gatctatttc
     7921 cgcgacgagc caccgctggg ctcgctcgag gccgcacagc agctggggcg caacgatttc
     7981 cgccggcttg tcgcattcac cagcctgtcc aagcggtcga acgtgccggg catgcgatcc
     8041 ggcttcgtgg ccggcgacgc cgagatactg aaatcgttcc tgctgtatcg cacctatcac
     8101 ggcagcgcga tgagcccgac cgtgcaacgg gccagcgtcg tggcctggaa cgacgaggcc
     8161 catgtggtcg ccaaccgaga gcagtaccgt cgcaagtttg cccaggtgac cccgctgctg
     8221 gccgaagtga tggacgtggc gctgcccgac gccggcttct acctgtgggc cgacatttcc
     8281 cggctcatcc ccggcggtga cgacgtggaa tatgcccgtg gcctgctcgc tcaatacaat
     8341 gtggcggttc tgccgggaag cctgctggca cgcgaagcag gcggcgtgaa tcccggagca
     8401 ggccgcatcc gcctggccct ggtgtccgag gcggacagct gcctcgaagc cgcccaccgg
     8461 atcgtggccc actgccgatc gatgatcagc gcctgagggc cggtcctgcc ggctcgcagt
     8521 gagccagccg gtgtgtgttc ctctcctgcc acccttttac tgaactgtcc atgaccgcaa
     8581 acctccagag catcatcgaa gccgcgtggg acaaccgcgc caacctgacg accaccagcc
     8641 atccggaggt caatgcagcg gtcgaaacgg tgatctccga cctgaacgcc gggcgcgtgc
     8701 gcgttgccga ccgtcagggc gtcggccagt ggagcgtgaa ccagtgggtc aagaaagccg
     8761 tgctgctgtc gttcaggctg aacgacaacg agctgatgca cgcgggcgat ctcaccttct
     8821 tcgacaaggt gaagaccaag ttctcgcaca tcgacgaaag cgccgtgcgc gcttcgggcg
     8881 tgcgcatcgt gccgcctgcg gtggctcgcc gcggcagctt cctggcgaag aacgtggtgc
     8941 tgatgccgag cttcgtgaac atcggggcct acgtcgacga gaacacgatg gtcgatgcct
     9001 gggccacggt cggctcctgc gcgcagatcg gcaagaacgt gcacctgagc ggcggcgtcg
     9061 gcatcggcgg cgtgctggaa ccgctgcagg ccaacccgac gatcatcgag gacaactgct
     9121 tcatcggcgc ccgctcggag atcgtcgaag gcgtgatcgt cgaggagaac tcggtgatct
     9181 cgatgggcgt gtacatcggc cagagcacca agatctacga ccgcgccacc ggcgaggtca
     9241 gctacggccg cgtgccggcc ggctccgtgg tggtcagcgg caacctgccg agcgccgatg
     9301 gcacccacag cctgtattgc gccgtgatcg tgaagaaggt cgacgcgcag acccgcgcga
     9361 agaccagcct caacgacctg ctgcgcgcct gatactcaag ttgagcgtgg aagcaccgat
     9421 gccacaggcg ttgcaccgag ttcttggagc aagggagtcg tcatgagcgg gaacatggag
     9481 cgcgtgctgc gcctgatgtc ggagaagagc gcgtccgacg tctacctgtc ggccaacacg
     9541 ccgatcctga tcaagatcaa cgggcagatc atgcagctga gcgatcagcc gctgacgccg
     9601 gcgcagcccc ggcaactgct cgccgaggtg ctgaccccca agcagctcga ggaactcgac
     9661 gacaccggcg agctgaatgt tggtgtcggg ctgccagggg tcggaagctt ccggctcagc
     9721 ggcttcaagc agcgcggctc gatcgccgcc gtcttccgct gcatcccggt cgacattccg
     9781 acgctcgaaa gcctgcaggt gccgtccctg ctgaacacgc tcgtgatgga gaagcgcggg
     9841 ctgatcctga tggtgggctc caccggcgcc ggcaagagca ccacgctggc cgcgatgctg
     9901 gaacagcgca accagcagat ggccggccac atcctgacca tcgaggatcc gatcgaattc
     9961 ctgttcaaca acaagaagtc ggtcgtcaac cagcgcgagg tcgggcgcga cacgcagtcg
    10021 ctgcaggtcg gcctgcgcaa tgcgctgcgg caggcacccg actgcatcct gatcggcgag
    10081 atccgcgatc gcgagacgat gaccgcggcg atctcgtacg cgctgtcggg ccacctggtg
    10141 ctgtcgacgc tgcatgccaa caacagctat cacgccctgg gccggatcct gtcgttctac
    10201 acgccggaat cccgccccgc gctgctgtcc gacctcgcat caggcctgcg cgccatcgtg
    10261 tcgcagcgac tcgtgcgcgc cacggcgggc ggccgcatcc cggcggtcga ggtgatgctc
    10321 aacaccaaac tggtgtccga cctgatcacc gccggcgact tcggcggcgt gcgcgaggcg
    10381 atggaaaagt cgctggccga gggatcgcag accttcgagc aggatctggc ccgactgatc
    10441 accgacggca ccatctcgcg cgacgaaggc ctggccttcg ccgactcgcc gaccaacctg
    10501 atgtggcggc tgcagaacga catgtcgccg gtgtcccgga tcaccgcgaa gaaggaagaa
    10561 gtggacgaag gtgccagctt cacggaaatc acgctcgacg tgcgtccgga accatcgaac
    10621 acccgcgccg cgccgctgtc gcgcatgtga ccgcgcgatg gaccgccctc gcatcacacc
    10681 cccaatgacc atgcaaccgt cgacttcatg acactcctag atctgatcaa gcgcgaagcc
    10741 gcccgcctca agaaggcggg cgtttcattt gggcatggca ccgccaatgc cttcgacgag
    10801 gccgcctggc tggtgctgtg gagcctcggg atggacctcg atgcgctcga gacgcacgcc
    10861 aagcgcgagg catcggccga cgaggaggcc aaggcggttg cgctggtgac ccgccgcatc
    10921 gagacgcgac ggccagcggc ctacctgaca caggaagcct ggctgcagaa cgtgccgttc
    10981 tacgtcgacg aacgcgtgat cgttccgcgc tcgttcatcg ccgaactgct ggtcgatggc
    11041 gaaggccagg gcacgctcga cgcgtggctg tccgaccgca cgcagcacgt gctcgatctg
    11101 tgcaccggca atggcagcct ggcagtgatc gcggccctgg cgtacccgga gatcgaggtc
    11161 gatgcttgcg acctgtcgga cgacgcactc gcggtggcga agatcaacgt cgagcggcac
    11221 aagctgtcga agcgcatcac gctgatgaag tccgacctgc tcgacagcgt gcgcggaccg
    11281 tacgacctga tcctgtgcaa cccgccttac gtcaattcga gcagcatggc ggcactgccc
    11341 gatgagtacc gccatgagcc cgcgctggcg ctggccggcg gcgaggacgg catggacctg
    11401 gtgcgccgca tcgtcaacga cgccgtagcc aagctcaccg acatcggcgt gctggtgctc
    11461 gagatcggca acgaacgcgg gcatttcgag gccgcgttcc ggcgcctcga agtggcctgg
    11521 ctggaaacca gcgccggcga cgacgcggtg ctgctggtga cccgcgaggc gctgctgcgc
    11581 tggtccaagc gcagcgaaac ctggagggcg tgaagccatg atcacgctca aggacgtgac
    11641 cctgcgtcgc ggaacgaagg tcgtgctcga cgctgcctcg gtgacgctgc agccgggaga
    11701 gaacgtgggt ctggtgggcc gcaacggtgc aggcaagtcc agcctgttcg cgatgctgac
    11761 gaaccggctg cagtccgatg ggggcgaggt ctacatgccg ccgcgctggc gcgtcggcga
    11821 agtcgcgcag aacatgcccg agaccgatga cggggccacc gacttcgtac tcgagggcga
    11881 cacgcgactg gtcgaagcgc aggcgcagct cgccgccgcc gaggccggcg acgacggcca
    11941 cgcgatcgcc gatggccacc aggcgctggc cgacgccggc gccttcgatg cgcgaccgcg
    12001 tgcgcaggca ctgctgatgg ggctgggctt caagaacgag cagctcgacg ccccggtcaa
    12061 cagcttctcg ggcggctggc gcatgcggct gcaactcgcc cgcgcactga tgtgcccggc
    12121 cgacctgctg ctgctcgacg agccgaccaa ccacctggac ctcgacgcgc tggtctggct
    12181 ggaggcctgg ctcaagcgat tcgaaggcac gatgctggtc atcagccacg accgcgaatt
    12241 cctcgatgca atcacccacg tgacagtgca cctcgaggac gcgaagctga cgcgctacgg
    12301 cggcaactac accgcgttcg aggaaatgcg cgccgagcgc atggtgctgc agcaggccag
    12361 cttcgagaag cagaaggaga agatggccca tctgcagaag ttcatcgacc gcttcaaggc
    12421 caaggccagc aaggccaagc aggcgcagag ccgcgtgaag gcgctcgacc ggatggagaa
    12481 gctggcgccg atattgacgt cgcgcgagtt ctcgttcgag ttccgcgagc cgctgaacct
    12541 gccgaatccg atgcttagcc tgaaggacgt cgagtgcggg taccacgcac tcgaggaagg
    12601 cgggcccgac aagatcatcg tgcgcaacat caaccgctcg gtgctcgcgg gccagcgcat
    12661 cggcatcctt ggcgccaacg gccagggcaa gtcaaccgtg gtcaagacca tcgcgcgcgc
    12721 gcaggccgca ctcggcggca cgatcaccga aggcaagggc ttggcgatcg gctatttcgc
    12781 gcagcaggag atggacgtgc tgcgtcccgg cgattcgccg ctgaaccaca tgatccggct
    12841 ggcccgagag gtcagcccgc aggcgcgcga gcaggaactg cgcaacttcc tgggccagtt
    12901 ccgcttcgcc gccgacatgg tcaaccagcc ggtcggcacg ctcagcggcg gcgagaaggc
    12961 gcggctggtg ctggcgatgc tggtatggca gcgccccaac ctgctgctgc tcgacgagcc
    13021 gaccaaccac ctcgacctcg acacccgcga ggcgctgtcg atggcgctca acgagttcga
    13081 gggctcgctg atgctggtca gtcacgaccg cgcgctgctg cgcgaggtct gcgacgagtt
    13141 ctggctggtc gccgacggcg gcgtgcgccc gttcgacggc gacctcgacg actaccagaa
    13201 gtacctgctc gacacggctc gcgcacaggc caaggccttg cgggaggccg gcaacgccag
    13261 caagaaggcg gcggcgccgg cgcccgcatc ggcacccgtg gcgcccgccc cgaagccatc
    13321 gacggccgcc accgcgccgg ccaagcgcga agaccgcaag gccgatgcgc aggcgcgtca
    13381 gctgaagtcc gacgagctca agcccctgcg caaggaactg aaccgcatcg acaaccggct
    13441 cggcgcgctg ttcggcgacc gcgatgccct cgaggcgggc ttcgcaaccg gctcgatgtc
    13501 accggcgcag ctggccgaga gcggcaagaa gctcaaggcc atcaccgaag agatcgggct
    13561 gctcgaaggc cagtggctgg acctcagcac gcaggtcgac gagttgacgg ccaaggcggg
    13621 ctgaggctcg ccaagcctgc accctgaaga acggcctctg cgggccgttc ttcatgtggg
    13681 ttgatgttcc atcagcgcaa gcatcagctc gatgcgcgcc tgaaccggac tcaggtcttc
    13741 ggcgcccggc agtcggtcgc cagcgacagg caccagccgg ccgtcgccgc agcgcaggct
    13801 gcgcagaacc gcgatgcccc gggatcgggc ctccagcagg gccgcttcca gatcgtggtg
    13861 cacccggccg ttgccggtcc cggccacgac cagtccgtcg acacagcggg tgccctcggt
    13921 accgtcgccg cacaaggctc gcaccgccgc agcgccggct ccggcatggc tgaggacgat
    13981 ttcgacgcgc ggccactgcg ccaccggatg cgtgagcgcg gcggcgaccg gcgcggtgga
    14041 atccatcgca gcgaatgcag ccgtgcggtc cgcagccgca acgaaccgac cgtcctcgat
    14101 cgcccccagc agcccggcct cgcccgagct gaacgcgtcg agccgagtcg gatgcacctt
    14161 gcgcacgttg cgggcgccgt gcaccgtgcc ggacagcacc gcgagcaccc cggtggcgcc
    14221 gggatctcgt gccagcgtca ccgcatcgag caggttgcgc ggcccgtctg cctcggaaga
    14281 agtggccggc cgcatcgcgc cggtcagcac gaccggcttc gcaggtgcca gaacacgctg
    14341 caggaagtag gcggtttcct ccagcgtgtc ggtgccgtgc gtgacgacga cgccgagcac
    14401 ttcggcgcga gccaggtggt gcgccacgcg ctgcgccagc gactgccaga ttgcgtgggt
    14461 catgtccttg ctgtcgatct gcgcgacctg ttcgctttcg atcggcaggc cagccagctc
    14521 cggcaggccg gccaacaact gcacgatgcc gagctgcgcc gacttgtagc cgaggtggtc
    14581 cccaggcgag tccgcggtgc ccgcgatcgt cccgcccatc gccaggacga cgaccgtgcc
    14641 ggcgccgcca accgggtgtg tcgggtgggc gggtttgatg cgggggtgca ctgccacggg
    14701 ccggattctg ggcccacgga tcgccgacac ggcagccgcc acgacgggcg cctgcgcggg
    14761 ggatcggaaa cgtgcggata cgcctcgaac tcaagtcgcg gccgcttacg tcgaaaaaag
    14821 gtcattgtca agcttcaatc gggaactcaa cgtgaacact tcctcgatcg caaccacgca
    14881 ggcactggcc tcgcagaccg gctattcgct gtccatccgt gcctcttcgg gcccggcacg
    14941 accggagggc accgacgatt catccgaggt gcgccgcctg cgtcacggcg atcacgagca
    15001 ccaccacgat cacgaccatc gggcgccgcg ctctgacgat cagggctatt cgggcagcgc
    15061 gttcacctcg ttgaagctga agatcgagga gaccttctcg tcgctgaccg gtcgcaccgg
    15121 cacggatgcc gaggactcga cttcatcgtc ggagttcgaa ggcgagttcc aggcccggat
    15181 ccagttctcg ggaccggacg gcgagttcaa ggccaagctc aagttcagtc tggagggcga
    15241 ctcggcgacc gccggcgccg acttcgccag cgcaatgcag ggcttcgcgc aaacgctgtt
    15301 tgccgcactg aacgcgctgt acggcggcgg cagtacgcca gcactgccgg gccccacaga
    15361 aagcacggcg cccgtgggca ccgaccccgc ggccacgccg gcgctgcctg ccccaccgcc
    15421 tgcgcctgct ccggtggcgg acgagacggt tgccgcagcc ccggcgccga ccgcgaaccc
    15481 ggaaccggtc gtggttgcgc ctccagcggc tccttcgaca cccaccacgg gcagcttcag
    15541 tccgacgacg acgtcgttct cgatcaagct gcgcgccacc tatgactcgt tcgagaacaa
    15601 cctcgggccc ctggtcaacc agctggctca gccggatgtg ggcgacgcat tcccggcctt
    15661 gacgtcgctg ctgcgggacc tggcggaccg cttcgggcag ctggtctcgc aggacccggc
    15721 ctccgcaacc agcgcgccca ccctgcagga cttcctgaaa gcactgtcgg gatccttctt
    15781 caacccccag cccgaggcat ccgcagaggc ggacgcagct gatccatcga cacctgccct
    15841 gccgactgcc gagaccggcg cccagcccac tgatcccgtc gtcgcggaca caccgaccgc
    15901 gcccgccccg acgagccaac gcttcacggc gatggcccag tacaagcagg tgctgaccta
    15961 cagcgacgcg ggctcgtcgc tcagcctgca gaccagcatg agtgcacgcc tggtgtacga
    16021 agtcgcctga tcacctgcag cgtgcgccgg gagcaccggc aacctttgca ttttggtgaa
    16081 ggctggatga aaatacagtt gactggacaa atcgccagtc cactgccagc cacgggtgcc
    16141 gccatgcgac acccagccca ccgagatgcg ccatgcaaga tgccccgaag ctgaccgatc
    16201 gacagcagca gatcctcgat ctggttcaaa gcgccatcga gcgcaccggg gcaccaccca
    16261 cccgcgccga gatcgcagca gagctcggct tcaagtcagc caacgcggct gaagagcacc
    16321 tgcaggccct ggcccgcaag ggcgtcatcg aactggtcgg tggcacctcg cgcggcatcc
    16381 gcctgaagtc cgagaccctg cgcgcgctga acgagatgcg cggcaagcag ttcagcctgc
    16441 cgctgcccag cctgtcgcag ctcacgctgc cgctgatcgg tcgcgtggct gccggcagcc
    16501 cgatcctggc gcaggagcac atcgaccaga cctacctgat ggaggccagc atgttcccgc
    16561 gccggcccga ttacctgctg aaggtgcgcg gcatgagcat gcgcgacgcc ggcatcatgg
    16621 acggtgacct gctcgcggtg cagaaggcac gcgacgcgaa gaacgggcag atcgtcgttg
    16681 caaggctggg cgacgaggtc accgtcaagc gcttcaagcg cacccgcacc acgatcgaac
    16741 tgcaccccga gaacccggac ttcgagccca tcgtgatccc gttcggcgat gtcgacttcg
    16801 aactcgaagg cctggccgtc ggcctgatcc gcaacaacat gatgatgtag ctgcggcgcg
    16861 ctccgacagc gggttctggc cggcgatcgc cggccttttc aatcgatcgc ctgcggtggc
    16921 ggggacaaga attcgtgtcg aacgtcacgc gccgcgtgcg gagtcgatcg acatgttgaa
    16981 ccgctggaca caccgactgc aatccacctg ggccaaacgg ggccgaccgg catccgacag
    17041 gcaggaggcg cagcggccgc ttcaacccgc gacggcacgc cacgtcgaga tctgcccgcc
    17101 ggatcgtctc taccggcgat cacgctggcg caggggcctg cgtgactggc tcaacaccgg
    17161 ctgggacgtc agctcgcccg cagcgctgac cgtcgagcga acgccagcca cgcccgccag
    17221 cgaggccgag ccgacgccgt tcgccgacat ccgccaggaa ttcgcgaatg cgctgaacga
    17281 catcgacacc gatgccgcag cgcaactgcg cgcacagatc gaacgggccc gctcaccccg
    17341 cgaactgtgg cacctgcgat ccgccctgtt cacgatggtg gccaggcatc acagcgagtc
    17401 gcaggctcag tcgcgcatgg agcgcctgaa ccgccacttc ccgaatcgca gcccggcctc
    17461 cgggcctgga acgcgctgac atgcctgcgc atcgtcacgg cccgcagccc gaccacgaac
    17521 tctggcgcgc ggtacacgag cggcccgcgg cgccgactcc ctggcctttc gaccgcggga
    17581 gcccggccat cgcgctgcac ggcatgcccg ccgaccgcct ggcgatgacc gcctcgctgc
    17641 gatccatcca gctccaggcg caccaccggt cgcgctgacc gaggcgcgtc agtcgcgccc
    17701 gatgaaatcg aacttgccga tctcgacgcc gttgtgccgc agcagggcat aggcgatcgt
    17761 cacgtggaag tagaagttcg gcagcaccca gtgcttgagg taggcctcgc ctgcgaactt
    17821 caacggcgac gcgccgcgga tcgggacctc gatctccctg gcctcggtgc cgtcgatctg
    17881 gtccgccgca accgacttga tgtaatcgag cgtctttgcg atgcgctgcc tcagttcggc
    17941 catcgtcgtc tcgttgtcgt cgaacttcgg cgcgctgtcg ccggacagcc gtgcgacgcc
    18001 gtacttggcc atgtcacagg cgatctggat ctgcttggtg aacggcagca tgtcaggcgc
    18061 gagccgcgcg gcgagataga ccgacgtgtc gaatttcttc gccacggcgt gcgcctcggc
    18121 ggcgtcgagc caccgcaaca ggttgccgag catcttctcg aagaccggca cgctcgccga
    18181 gtacatggaa atggacatga aagactcctg tcggatggac cgggtgattc cggtggcgcg
    18241 cgatgctact gcgcaagggc tcggcctgcc gacgggcaca attgaagggt cttttcagcg
    18301 cccattgccg tgaacatcat catcctcgac gactaccagg acgcggttcg caagttgaag
    18361 tgcgccgaaa agatggaggc cctgcacgcc aaggtcttca ccaacaccgt caaggggatc
    18421 ggacagttgt cgatccggtt gcgcgacgcg gaggtcctgg tgctgatccg cgagcgcacg
    18481 cacttcccca ggcaactgct cgagaagctg ccccggctgc gcctgatctc ccagacagga
    18541 aaggtcggct cgcacatcga cgtcgaagcg tgcacccgcc tcggcatcgc agtggccgag
    18601 ggcgttggct ctccggtcgc gccggcggag ctgacctggg cgctgatcat ggcggcgatg
    18661 cgccgcgtgc cgcagtacat cggcaacctg aagcacggcg catggcagca gtcgggcctg
    18721 aaagcctcgt cgatgcctgc caacttcggc atcggcatgg tgctgaacgg caagacgctg
    18781 ggcgtctggg gctatggccg catcgggcag ctggtcgccg gctacgcacg ctgcttcggc
    18841 atgcgcgtga tggtgtgggg cagcgaatcg gctcgcgagc gcgccgaact ggatggtcac
    18901 gcctcggccc actcgtgcga gagcttcttc gaggaatgcg acgtgctgtc gctgcatctg
    18961 aggctcagcg aaacgacacg cggcgtggtg aagatcgagc acctgtcgcg gatgaaaccc
    19021 acgtcgctgc tggtcaacac ctcgcgcgcc gagctgatcg aggaaggcgc gctggtgtcg
    19081 gcgctcaacc ggggccggcc cggcatggcc gcggtcgacg tcttcgagag cgaaccgatc
    19141 ctgcagggac acccgctgct gcgactcgag aacgcggtgt gcacaccaca catcggctat
    19201 gtcgagcagg acagctacga ggagtacttc ggagctgcgt tcgacaacgt ggtgagcttc
    19261 gtcgaaggtc gaccgaccca catcatcaac cccgaagccc tgaaggtgca gcgctgagcg
    19321 catcgagcag cgaaaccgag cttgccgcgc cccggtcgaa cgccgatctg ttcctgacgt
    19381 tcacgtggct cgcgctgcag ggtttcggcg gcgtgctcgc ggtggcacag cgcgaactgg
    19441 tcgaacgcaa gcgctggctg acgcgcgaac agttcgtcga atacctgtcg gtcggccagg
    19501 tgctgcccgg ccccaacgtt gtcaacctgt cgatgatgat cggcgaccgt ttcttcggcc
    19561 tgcgcggcgc gatgtccgct ctggcgggca tgctggcgat gccgctggtg atcgtgctgc
    19621 tgctggcctc gctgtacggc cgttatgaaa gtcagcctgc ggtgcagggt gcgttgcgag
    19681 gcatgggagc ggtggccgcc gggctggtgc tggccaccgc actgaagctg ctgcccacgc
    19741 tgcgatcgag cgccctcggc gtacccgccg gcctgggatt cgcggcagtc gctgcccttt
    19801 cggtcacggc gtggcactgg ccgttgtggt gggtggtcac gggcatcggc tcgctggctg
    19861 tcgcactggc atggtggcga ttgcggccat gagcacggcg ggccaaacca cgtgcccagg
    19921 cagcccggcg gaggcacggg ttcctgttgt ctgggggcat cgatgacgcc ggtggatctg
    19981 cccgccctgt tcggccattt cctgctgttg agcctgctgg cggtcggcgg ggcgatcacc
    20041 acggcaccgg acatgcaccg ctacctggtg accgaacacg gctggatcag cgacgcgcag
    20101 ttcaccacat cggtcgcgct ggcgcaggcc gcgccggggc cgaacctgct gttcgtggcg
    20161 gtgctcggct ggaacgtcgc cgggccgatg ggcgccgtgg cgacgatgac aggcatcctg
    20221 ctgccgtcga cgctgctgac gctgtgggcc acccgctggg gccaggcgcg gcgcgacacg
    20281 cgcggggtgc gggcgttcac caccggcatg gcgccgttga cgctcgggct gctggtggca
    20341 accggttggg tgctgacgga gccggtgcgg atgcaggttg gcgcgtgggc gttgatcgcc
    20401 atcacggtgg tggtgatggt tcgcacacgc gtcagcccga tgtggctggt ggcgctaggc
    20461 gccgtcgtcg gggcgatggg gtacgcgtga cccctgctct tcgccaggca aacgcctgga
    20521 gcaccgagat gtaccgcgcc gggtttcggc ccggctgccg agtaactttc atttgctggc
    20581 ccaaatgaaa gtcaccaaag caaaaggcct gaacaccagc cgatctgggt gtcacgctgc
    20641 ggcctgaagg ctgactgcag aggctctggc acgagaaccg acaccggact accgcctcgg
    20701 cttcctcgct aatgccagtc cgcttgttct cgcttcggct cgaccctgac gggtcctcgc
    20761 ggcgatgacc cgcagggtcg agccgtatcg ataccgagtt gactgaatgt gagtgaggaa
    20821 accgattcgg tagctcggcg cttgttctcg tgccagagcc tttgaggtag gtgtgcagcc
    20881 gaagcgagcc gatcaaatcg gctggtgttc ggcccctctt ctttggtgac tttcttctgg
    20941 ggcagcagaa gaaagttac
//
LOCUS       DAOORV010000059        20931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3096_length_20931_cov_7.891550, whole genome shotgun sequence.
ACCESSION   DAOORV010000059 DAOORV010000000
VERSION     DAOORV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_20931_cov_7.891550"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1580)
                     /gene="gltX"
                     /locus_tag="PKV56_09585"
     CDS             complement(165..1580)
                     /gene="gltX"
                     /locus_tag="PKV56_09585"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOX68103.1"
                     /translation="MTRKVRTRIAPSPTGFLHLGTARTALFSWAFARHHGGDFILRIE
                     DTDEARSTQEAVDQIVAAMKWLNLDYDEGPFFQMQRIERYREVIGQMLAAGSAYPCYC
                     TPAELDAMREAQRARGDKPRYDGRWRPEPGKVLPVAPEGVKPVIRFRNPIDGAVTWDD
                     MVKGPITISNGELDDLIISRPGADAQTLIGTPTYNFCVVVDDWDMQISHVIRGDDHVN
                     NTPRQINILQALGAVLPVYGHVPMILGPDGDKLSKRHGAVSVTQYEDAGYLPEAMLNY
                     LARLGWSHGDEELFTREQLVTWFDGHHLARSPAQWDVAKLNWVNAHYIKTLPESSLAA
                     HVSSRFASRGIDVPVDDHLVRLCALFKDRCATTMELAEWLAMYFMDVEPRDEDLATHV
                     TDAVMPAVQSLRERLTDIEWSKPAISAAIKEVITAHGIKMPQLAHAVRVLVCGRAQTP
                     SIDAVLELFPREVVIARLQKR"
     gene            complement(1605..2843)
                     /locus_tag="PKV56_09590"
     CDS             complement(1605..2843)
                     /locus_tag="PKV56_09590"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOX68104.1"
                     /translation="MSTKKTIPRVVLPEGTRIDTLAVREGLPRSQWGENSEALFLTSS
                     FVQPDAATAAARFANEEEAFIYSRFTNPTVTMFERRLAAMEGAEACIATASGMAAILL
                     TAMALLKAGDHVICSRSVFGSTITLLGREFSKFGVETTFVSQTDVEDWRRAIRPTTRL
                     LFAESPTNPLTEVCDIRALAEVAREANALLAVDNCFCSPALQRPIEYGADLIIHSGTK
                     YLDGQGRVIAGAVCGPEQLINDKFVPVMRSAGMTLSSFNAWVVLKGLETLSIRMQAQS
                     ERALKLATWLEQQPQIERVFYPGLKSHPQHELAMAQQSGCGGAVLSFVVRARAPGDAA
                     AARLTAFHVIDSTRVCSITANLGDTKTTITHPASTSHGRLTEEQRQAAGITQGMVRIA
                     VGLDDVEDLKADLLRGLNSL"
     gene            complement(2843..4366)
                     /gene="purF"
                     /locus_tag="PKV56_09595"
     CDS             complement(2843..4366)
                     /gene="purF"
                     /locus_tag="PKV56_09595"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOX68105.1"
                     /translation="MCGIVGVISKAPVNQLIYDALLLLQHRGQDAAGIVTMQDSRCYM
                     HKARGMVRDVFRTRNMRGLLGPVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAATLKKELARIDRRHINTDSDTEVLINIFAHELEVAARDLPLTPDIIFKAVSA
                     VHKRLKGSYAVVALIAGFGLLAFRDPFGIRPLCFGEGDTPDGRQVMVASESVALEGTG
                     HVMTRDVAPGEAIFITSEGQVHAHQCAENPSLHPCMFEYVYLARPDSIMDGISVYQAR
                     LNLGETLAQRLISTIPPSEIDVVIPIPESSRPSAMQLAQKIGKPYREGFVKNRYVGRT
                     FIMPGQGVRKKSVRQKLNAIGVEFKDRNVLLVDDSIVRGTTSKEIVQMAREAGARKVY
                     MASAAPPVRFPNVYGIDMPTNSELIAHNRSIEEIRQFIGADALIYQDVNAMKRVVGAL
                     NPLVAGFEASCFDGHYITGDITAADFDEIATQRRSQVEEEDSADRSRLALQNAAEQA"
     gene            complement(4448..4957)
                     /locus_tag="PKV56_09600"
     CDS             complement(4448..4957)
                     /locus_tag="PKV56_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOX68106.1"
                     /translation="MSTMTASWNAVDIFMLAALLLSMLVGAWRGLVFELMSLVGWFVA
                     YLAAQWLTPSLSVHVPIGAPGSAINHAAAFIATFVVALLAWGLLSRLLKMLIHATPLG
                     LVDRLFGGAFGVLRGVVLLLVVAAVVTRTPWAQSPAWQQSTSAAWVKVLLVGLKPSLP
                     TTISDQLRL"
     gene            complement(4998..5846)
                     /locus_tag="PKV56_09605"
     CDS             complement(4998..5846)
                     /locus_tag="PKV56_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX68107.1"
                     /translation="MGLTSFFKRKTPGDAPASTTRSAPTFDAVEKVRTRTRQRLIGAV
                     VLLTIGVIGFPLIFESQPRPIPVDIPIDIPRQDNAPALVLPAPRREVTPAPAETTASR
                     GADGTDVAATGVSRESPADLMPSSEPSASGATSAPPAAASTVASGKVEVLPSRPAPAA
                     EPSASARAPLAVSKAASEAPAARPARNAATAVDITSDAAPASAPEATGRFVVQVGAFA
                     DPAAARELRAKVEKLGLKTYTQVAETSAGKRIRVRLGPFATRAEAEKVQARSKDAGIA
                     AVVLTL"
     gene            complement(5888..7195)
                     /gene="folC"
                     /locus_tag="PKV56_09610"
     CDS             complement(5888..7195)
                     /gene="folC"
                     /locus_tag="PKV56_09610"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOX68108.1"
                     /translation="MSSPLPTSLAAWLARCEKLHPKEIDMGLHRVEQVRARLDLNFDV
                     PVIAVAGTNGKGSTCAMLESIALRAGYRVGLYSKPHLVHFEERCRVNGEMVTESELLV
                     HFEAVELARGDIGLTYFEFTTLAIARMLSQATLDLVILEVGLGGRLDAVNAFDADCAV
                     ITSIDLDHMEFLGPDRESIGREKAGIMRAGRPVIVSDPVPPQSVLDRARDIGADLRRF
                     GVDFNHSGDQQQWAWAGRSKRYNGLAYPGLRGANQLINASGALAVFEALGDRLPITAQ
                     AVRQGFAMLDLPGRFQIVPGQPTLVLDVAHNPHAVAALAQNLDRMGFHPRTHAVFGAM
                     HDKDLAGIFRRLAPIVDTWCFTDLPISRAATAEHLKTVHEQLSLKGPGPIAVSTHADP
                     MQALNHALEVADPTDRIIVFGSFLTVGGVLKHGLPRLGSSHGV"
     gene            complement(7223..8095)
                     /gene="accD"
                     /locus_tag="PKV56_09615"
     CDS             complement(7223..8095)
                     /gene="accD"
                     /locus_tag="PKV56_09615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOX68109.1"
                     /translation="MSWLEKLLPTKIQQTDPSERRSVPEGLWIKCPSCETVLYKTDLE
                     QNINVCPKCGHHHRMGARARLDAFLDPEGRYEIGQEVVPVDALKFKDSKKYPERLKEA
                     LEATGETDALVVMGGAIHSIPVVAACFEFQFMGGSMGSVVGERFVRGVETAVEQKTPF
                     ISFTATGGARMQEGLLSLMQMAKTNAALTRLAKAKLPYISVLADPTMGGVSASFAFMG
                     DVVIAEPKALIGFAGPRVIENTVREKLPEGFQRAEFLLHTGAVDMIIDRRELRTSIAK
                     MIAMLQRQSADALA"
     gene            complement(8092..8907)
                     /gene="trpA"
                     /locus_tag="PKV56_09620"
     CDS             complement(8092..8907)
                     /gene="trpA"
                     /locus_tag="PKV56_09620"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOX68110.1"
                     /translation="MSRIAATFEVLKSQRRKALIPFITAGDPHPESTVDILWAMADAG
                     ADVIELGVPFSDPMADGPVIQKAAERALLHRIGMPQVLDYVRRFRERNHSTPVVLMGY
                     ANPIERYGVDRFVADAKAAGVDGVLVVDYPPEECEAFARTLHSHQMDPIFLLAPTSTE
                     QRMKEVGRIASGYVYYVSLKGVTGAGHLDIGAVESMLPRIREHVHVPVGVGFGIRNAA
                     TAKAVAAVSDAVVIGSALVQLLEHETPAQAAAVAGRFIAEIRAALDTLTGENA"
     gene            complement(8904..10169)
                     /gene="trpB"
                     /locus_tag="PKV56_09625"
     CDS             complement(8904..10169)
                     /gene="trpB"
                     /locus_tag="PKV56_09625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOX68111.1"
                     /translation="MFDYHQPDATGHFGPYGGTFVAETLIHALDELKEAYAQAQADPE
                     FMAEFRSELAHFVGRPSPIYHAARISRELGGAQIFLKREDLNHTGAHKINNTIGQAML
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSADVLRQSPNVYRMNLLGAR
                     VVPVESGSKTLKDALNEALRDWVTNVESTFYIIGTVAGPHPYPMMVRDFQRVIGDECL
                     VQMPEMIGRQPDAVIACVGGGSNAMGIFYPYIEHEGTRLIGVEAAGLGIETGHHAASL
                     SAGSPGVLHGNRTYLLQDANGQIIETHSVSAGLDYPGVGPEHAYLKDIGRAEYVGITD
                     DEALKAFHLLCRNEGIIPALESSHALAYAMKIAPTMRSDQSLLVNLSGRGDKDIGTVA
                     DLSGAEFFCRPSCRGQTVKGGSSPVKGPK"
     gene            complement(10184..10831)
                     /locus_tag="PKV56_09630"
     CDS             complement(10184..10831)
                     /locus_tag="PKV56_09630"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOX68112.1"
                     /translation="MNSRTRIKICGLTREQDIDDAVEAGADAIGFVLYEKSPRFVDVR
                     RATELARRLPAFVTPVGLFVNADAELIAEATAAMPQLLLQFHGDETPGACQAPGRPYL
                     RAARMDAGFDLLKFALSHPDAQAILLDAHIDGYGGGGKVFDWSLIPRNVSRPLVLSGG
                     LHAANVIEGILRVRPWAVDVSSGVEQAKGVKDALAMRRFCEQVREADARIAANEN"
     gene            complement(10842..11627)
                     /gene="truA"
                     /locus_tag="PKV56_09635"
     CDS             complement(10842..11627)
                     /gene="truA"
                     /locus_tag="PKV56_09635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOX68113.1"
                     /translation="MNRLALGISYRGGAYCGWQRQSSAVSVQGCVERALSQFVDLPMT
                     VTCAGRTDAGVHALNQVVHLDAPVDREEVSWVRGTNRYLPADVAIQWCQCVDSEFHAR
                     FSAIGRRYSYLILESPVRPSLEAGRVGWTFRPLDGESMRQAAALLLGEHDFSAFRSSQ
                     CQAASPVKQMRSIEISRRGAYWRLDFDAGAFLHHMVRNIVGCLTAVGNGSRPVSWMGE
                     VLASRRRELAAPTFSPDGLYFTGPYYDDRHGIPKRPPATDWLP"
     gene            complement(11671..14178)
                     /locus_tag="PKV56_09640"
     CDS             complement(11671..14178)
                     /locus_tag="PKV56_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOX68114.1"
                     /translation="MGLGDALKNTTQQLGRFALSSVAVAALSLGAVNAWALGLGRLSV
                     QSSLGETLRAEIDVTSLTPEEAGNLKIRIAAPDTYRASGVDYNVVLPSTQATLLKRAD
                     GRPFLRLTSDRVVQEPFVDVILELSWSTGRLVREYTLLFDPPTDRPQAAASAPPSAVA
                     TAPVISAPAPAPRPARAAAPAAPVAQAPAPAPERAATPAATPAAPSEPVGDTYRVRSG
                     DTLTGIASRTRRGSVSLDQTLVSLYRGNPKAFVGENMNRLKAGALLSVPSAEAAAAVD
                     AAEARQLIQAQSADFGSFRQRLASGVPAVQADVPARKDAGTVQASVDDRKTTNAVAPD
                     KLTLSKGASAAKPGAPEVKISKDKEKQDAAVRVAELSKNVEELKKLSGATNANGGTPA
                     AAAPPAPAPAASVAPAPATAPVVASMPAEPPATVVAEEKKAAAAASASKPAPAKAAAP
                     QEAEEPGFLSELFEGNALMLGVGGGAILALLGFGVYRARRAKLDTGETSFLESRLQPD
                     SFFGASGGQRIDTREAASGAPSSMSYSLSQLDAIGDVDPVAEADVYLAYGRDLQAEEI
                     LKEAMRSSPERMAIRTKLLEVYAKRRDTKGFELLATQLFALTGGSGEDWEKAQELGLD
                     IDPDNTLYQPGGSPDSMSASDSGLMSEPLGASTMPQSVLPTQSSFNETPTEVLGNASE
                     GAAEEIDFDLDLDALESAPAAVAPPSPPPLESFDFEPSPTPSAAQAFYPSAAAPGPDS
                     KSPLEFDMSSISLDLDAPAAPASAGATDIEIETDGVVDDEADDDNADPLARKLELAEE
                     FRQIGDTEGARDLLQEVANKAEGTLKAKAQGMLADLD"
     gene            complement(14392..15546)
                     /gene="asd"
                     /locus_tag="PKV56_09645"
     CDS             complement(14392..15546)
                     /gene="asd"
                     /locus_tag="PKV56_09645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOX68115.1"
                     /translation="MKLVGLVGWRGMVGSVLLDRMQQEGDFPLIEPMFFSTSNAGGKA
                     PVQARNEATLCDAFDIDALKRCDIIITAQGGDYTTEVFPKLRAAGWAGHWIDAASTLR
                     MKSDAVIVLDPVNLPVIQNALARGGRNWIGGNCTVSCMLMGVGALYKAGLVEWMSTQT
                     YQAASGGGAQHMRELLTQFGTLNAEVRGLLDDPKSAILEIDRRLLAKQRSLTESETAQ
                     FGVPLAGSLIPWIDKDLGIGKDRDDEGFGVSREEWKGGAETNKILGQGDGFDQPAVPV
                     DGFCVRVGAMRCHSQSLLFKLKKNVPLADIEQLISNDNAWVKVVPNNREDTIRQLTPV
                     AVTGTLGIPVGRLRKMAMGPEYLGAFTIGDQLLWGAAEPLRRMLRLLIEA"
     gene            complement(15586..16662)
                     /gene="leuB"
                     /locus_tag="PKV56_09650"
     CDS             complement(15586..16662)
                     /gene="leuB"
                     /locus_tag="PKV56_09650"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOX68116.1"
                     /translation="MKIAILPGDGIGSEIVAEAVRVLEVLDLRFETESALVGGAAYEA
                     HGHPLPDSTLKLAQDADAVLFGAVGDWKFDSLERSLRPEQAILGLRKHLQLFANFRPA
                     ICHPQLTHASSLKPELVAGLDILIVRELTGDIYFGQPRGRRLSPDGAFEGQPEAFDTM
                     RYSRPEIERIAHVAFQAARKRSKRVTSVDKANVLETFQFWRDVMIEVHAQYPDVELDH
                     MYVDNAAMQLVKAPKKFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLNAANRGLYE
                     PSHGSAPDIAGKNIANPLATILSLAMMLRFSLNQPEAAARIEAAVTRVLESGLRTADI
                     WSEGTQRVGTREMGSAVVAAVAAA"
     gene            complement(16741..17385)
                     /gene="leuD"
                     /locus_tag="PKV56_09655"
     CDS             complement(16741..17385)
                     /gene="leuD"
                     /locus_tag="PKV56_09655"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOX68117.1"
                     /translation="MNPFRVHQGLVAPMDRENVDTDAIIPKQFLKSIKKSGFGINLFD
                     EWRYLDAGFPGQDPAARRPNPDFVLNQSRYRGASVLIARKNFGCGSSREHAPWALDQY
                     GFRVVIAPSFADIFFDNCFKNGLLPIVLPESQVASLFDEVAAFVGYQLTVDLERQVIV
                     KADGTELPFEVQAFRKYCLLNGFDDIGLTLRHADKIKAYEAERLLKKPWLAHSL"
     gene            complement(17393..17521)
                     /locus_tag="PKV56_09660"
     CDS             complement(17393..17521)
                     /locus_tag="PKV56_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOX68118.1"
                     /translation="MKRTLFALAALVVFLTGCNTVEGIGKDVQRAGEVVEDAAKKK"
     gene            complement(17604..19013)
                     /gene="leuC"
                     /locus_tag="PKV56_09665"
     CDS             complement(17604..19013)
                     /gene="leuC"
                     /locus_tag="PKV56_09665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOX68119.1"
                     /translation="MSRTLYDKLWDEHVVHTEDDGTTVLYIDRHLVHEVTSPQAFEGL
                     DLAGRKVWRLSANLAVSDHNVPTTDRSNGIADPISRLQVDTLDANCDRVGITQFKMND
                     RRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLA
                     KKAKNLLIRIDGVLSPGCSAKDLVLAVIGRIGTAGGTGYTIEFAGSAIRALSMEGRMT
                     VCNMAIEAGARAGLIAVDDTTISYVKGRPFTPSGVEWDQAVAYWRTLKSDPDAIFDAL
                     VEFDAATIRPQVTWGTSPEMVLSIEDRVPDPEREKDANKRGAMERALTYMALTPNKPI
                     ADILIDKVFIGSCTNSRIEDMREAAAVVRKLGRKIASNVKLALVVPGSGLVKEQAERE
                     GLHTVFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAAIEGHFVDVRRLL"
     gene            complement(19154..20464)
                     /gene="gltA"
                     /locus_tag="PKV56_09670"
     CDS             complement(19154..20464)
                     /gene="gltA"
                     /locus_tag="PKV56_09670"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOX68120.1"
                     /translation="MTPSDVKATLSFSDGSPSMDLPIFKGSVGPDVIDIRKLYGQTGK
                     FTYDPGFLSTASCNSKITYIDGDKGELLYRGYPIEQLATKCDYLETCYLLLKGELPNA
                     DQRVEFTKRVTNHTMVNEQMQFFLRGFRRDAHPMAILTGLVGGLSAFYHDSTDINNPE
                     HREVSAIRLIAKMPTLVAMAYKYSVGQPYIYPKNDLSYAGNFMQMMFATPCESYVPND
                     VLVRAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAA
                     LNMLEDVQKMGGVEKIGEFIKQVKDKNSNVKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     GAMGKNNDPILKLAMALEKIALEDDYFVSRKLYPNVDFYSGIVQRAIGIPVSLFTAIF
                     ALARTVGWIAQLNEMISDPEYKIGRPRQLFNGSPSRDVKPIGER"
     gene            complement(20520..20786)
                     /locus_tag="PKV56_09675"
     CDS             complement(20520..20786)
                     /locus_tag="PKV56_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOX68121.1"
                     /translation="MDALIDDRALSKLRWRCRRGLLENDLFVERFFDRYQSTLTQRQA
                     DGLMVLMDLSDNDLLDLLLARQQPEGELLNDNILEVLRLMREPR"
     gene            complement(20812..>20931)
                     /gene="sdhB"
                     /locus_tag="PKV56_09680"
     CDS             complement(20812..>20931)
                     /gene="sdhB"
                     /locus_tag="PKV56_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733632.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOX68122.1"
                     /translation="PYRLFRCHTIMNCVDVCPKGLNPTKAIGKIKEMMIRRSV"
BASE COUNT         3726 a         6741 c         6714 g         3750 t
ORIGIN      
        1 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatac ccccctttgc
       61 tgccagttcc tgtaacccct gacatccctt ggggaagtga cacggagatc gatttctgtg
      121 tcgcttcgcg ctgttcaagc gagaccaaga gtatagccag ctttttaacg tttctgcaat
      181 ctggcgatca cgacttcgcg aggaaacagc tcgagcaccg catcgatgga cggggtctgc
      241 gcgcgaccgc agaccagcac gcgcaccgca tgtgccaact ggggcatctt gatgccatgc
      301 gcggtgatga cttccttgat ggcggcgctg atcgcgggct tggaccactc gatatcggtc
      361 aggcgttcgc gcaggctctg cacggcaggc atcaccgcgt cggtgacatg agtggcgagg
      421 tcttcatcgc gaggttcgac gtccatgaaa tacatggcga gccactcggc gagttccatg
      481 gtcgtcgcgc agcgatcctt gaatagcgcg cacagccgga ccaggtgatc gtcgacaggc
      541 acgtcgatgc cgcgcgaagc gaatcggctc gacacgtgtg ccgcgaggga tgattccggc
      601 aaggtcttga tgtagtgcgc attgacccag ttgagcttgg cgacgtccca ctgcgcggga
      661 ctcctggcaa ggtggtggcc gtcgaaccag gtgaccagtt gctcgcgggt gaacagctct
      721 tcgtcgccat ggctccagcc cagccgggcc aggtagttca gcatggcctc gggcaggtag
      781 ccggcgtctt cgtactgcgt gacgctgacc gcgccgtggc gcttcgacag cttgtcgccg
      841 tccgggccga ggatcatcgg cacgtgcccg tagacgggca acaccgcgcc gagagcttgc
      901 aggatgttga tctggcgcgg cgtgttgttg acgtgatcgt caccgcggat cacgtggctg
      961 atctgcatgt cccagtcatc gacgacgacg cagaagttgt aggtcggcgt gccgatcagc
     1021 gtctgggcgt cggcacccgg acgcgagatg atcaggtcgt cgagttcgcc attggagatg
     1081 gtgatcggcc ccttgaccat gtcgtcccag gtcaccgcac cgtcgatcgg attgcgaaag
     1141 cggatcaccg gcttcacacc ctcaggcgcg accggcagca ccttgcctgg ctccggccgc
     1201 cagcggccgt cgtaacgggg cttgtcgccc cgggcgcgct gcgcctcgcg catggcgtcg
     1261 agttcggctg gtgtgcagta gcagggatag gccgaacccg cggccagcat ctgtccgatc
     1321 acctcgcggt agcgctcgat gcgctgcatc tggaagaacg ggccctcgtc gtagtcgaga
     1381 ttcagccact tcatcgcggc gacgatctga tccaccgcct cctgcgtcga acgtgcctcg
     1441 tcggtgtctt cgatgcgaag gatgaagtcg ccaccgtgat gcctcgcgaa ggcccacgaa
     1501 aacaaggcgg tgcgggccgt tcccaggtgc aggaatccgg tgggggaagg cgcaatgcgg
     1561 gttcgaactt ttcgggtcat cggtcgggtg tcgtcagtgc gtcatcaaag tgaattcagg
     1621 ccgcgcagca ggtcagcctt gaggtcttcc acgtcgtcga gtccgaccgc gatgcggacc
     1681 atgccctgcg tgattccggc ggcctgccgc tgctcctcgg tcagacggcc atgcgaggtg
     1741 ctcgcagggt gcgtgatcgt cgtcttggta tcgccgaggt tcgcggtgat cgagcacacc
     1801 cgggtgctgt cgatcacgtg aaatgccgtg aggcgcgccg cggccgcatc gcccggagcc
     1861 cgcgccctga ccacgaacga cagcacagca ccaccgcaac ccgactgctg cgccattgcc
     1921 agttcgtgct gagggtgcga cttcaacccg ggatagaaca cccgttcgat ctgcggctgc
     1981 tgctcgagcc aggtggcgag cttcagggca cgctcgctct gggcctgcat gcggatcgaa
     2041 agcgtctcca gccctttcag cacgacccag gcgttgaacg aggacagcgt catgccggca
     2101 ctgcgcatca ccggcacgaa cttgtcgttg atcagctgct cggggccgca caccgcaccg
     2161 gcgatcactc ggccctggcc atcgaggtac ttggtacccg aatggatgat gaggtcggcg
     2221 ccgtactcga tggggcgctg cagggccggc gagcagaagc agttgtcgac cgcgagcagc
     2281 gcgttcgctt cgcgggccac ctcggccagt gcgcggatgt cgcagacctc ggtcagcgga
     2341 ttggtcggcg actccgcaaa cagcagccgc gtcgtcggcc ggatggcccg ccgccagtcc
     2401 tcgacatcgg tctgcgagac gaaggtggtc tcgacaccga acttcgagaa ctcgcgtccg
     2461 agcagcgtga tcgtcgagcc gaacacgctg cgcgagcaga tcacgtggtc gcccgccttc
     2521 agcaaggcca tcgccgtcag caggatcgcc gccatgccgc tggccgtggc gatgcaggcc
     2581 tcggcacctt ccatcgcggc cagacgacgc tcgaacatcg tcacggtcgg gttggtgaaa
     2641 cgcgaataga tgaaggcttc ttcctcgttg gcaaaacggg cagcggcggt ggccgcgtcg
     2701 ggctgcacga agctgctggt caggaacagg gcctccgagt tctcgcccca ctggctgcgc
     2761 ggcaggcctt cgcgcactgc cagcgtgtcg atgcgggtgc cctcgggcaa caccacgcgg
     2821 ggaatcgtct tcttcgttga catcaggcct gctccgccgc gttctgcagc gccaggcgcg
     2881 aacgatcggc cgagtcttct tcctcgacct gggaacggcg ctgcgttgcg atctcatcga
     2941 agtcggccgc cgtgatgtcc ccggtgatgt agtgcccgtc gaagcaagag gcctcgaatc
     3001 cggcgaccag cggattcagc gcaccgacca cacgcttcat cgcgttcacg tcctggtaga
     3061 tcagcgcatc ggcgccgatg aactggcgga tttcctcgat gctgcggttg tgggcaatca
     3121 gctcgctgtt ggtcggcatg tcgatgccgt agacgttcgg gaaacgcacc ggtggcgcgg
     3181 cagaggccat gtagaccttg cgcgccccgg cctcgcgcgc catctgcacg atttccttcg
     3241 aggtggtgcc gcgcacgatc gaatcgtcga ccagcagcac gttgcggtcc ttgaattcga
     3301 caccgatcgc gttgagtttc tggcgcacgg atttcttgcg cacgccctgc ccgggcatga
     3361 tgaacgtgcg gccgacgtag cggttcttga cgaagccttc gcggtagggc ttgccgatct
     3421 tctgtgccag ctgcatcgcg gaaggccggc tcgactcggg gatcgggatc acgacgtcga
     3481 tctcgctggg agggatcgtc gagatcaggc gctgcgccag cgtttcaccc aagttcagac
     3541 gcgcctggta gaccgagatg ccgtccatga tcgaatccgg ccgtgccagg tacacgtact
     3601 cgaacatgca cggatgcagg ctcgggttct cggcgcactg gtgcgcgtga acctgcccct
     3661 ccgacgtgat gaagatcgcc tcgccgggcg ccacatcgcg ggtcatcacg tggccggtgc
     3721 cttcgagcgc gaccgattcg ctggccacca tcacctgccg gccgtcgggc gtgtctccct
     3781 caccgaaaca caaggggcgg ataccgaacg gatcacggaa cgccagcagc ccgaatccgg
     3841 cgatcagcgc caccaccgca tacgaaccct tgagtcgctt gtgcaccgcc gacacggcct
     3901 tgaagatgat gtcgggcgtc agcggcaggt cacgcgccgc gacctcgagt tcatgggcaa
     3961 agatgttgat cagcacctca gtgtcgctgt cggtgttgat gtgacgacga tcgatgcggg
     4021 ccagttcctt cttcagcgtc gccgcattgg tcaggttgcc gttatggacc agcacgatgc
     4081 cgaacggggc gttgacgtag aaaggctgag cctcttcttc gctgtaggca tttccggccg
     4141 tggggtaccg cacctggccc agacccaccg ggcccagcag gccgcgcatg ttgcgcgtgc
     4201 gaaacacgtc gcgcaccatg ccgcgcgcct tgtgcatgta gcagcgcgaa tcctgcatcg
     4261 tgacgatgcc cgcagcatcc tgaccgcgat gctgcagcag cagcaaggcg tcatagatga
     4321 gctggttgac cggcgccttt gatatcactc cgacgatgcc gcacatgaaa tcactcccgg
     4381 ttcaagaact tgcgaggaca cttcgggtcc gtcgcgcaac tgccattgtc tgcgggtcgg
     4441 gcccgcttca cagccgcagc tgatcagaga tcgtcgtggg caggctcggc ttcaatccga
     4501 ccagcaacac tttcacccag gcggcgctgg tcgattgctg ccaggcaggc gactgggccc
     4561 agggtgtgcg tgtcaccacc gcagccacca cgagcaacag caccacgccg cgcaacacac
     4621 cgaacgcacc accgaacaac cggtcgacca gacccagcgg tgtcgcgtgg atcagcattt
     4681 tcagcagcct cgacaagagg ccccacgcca gcaacgcgac gacgaacgtc gcgatgaacg
     4741 cagcagcatg attgatcgcc gagccaggcg caccgatcgg cacgtggaca gaaagcgacg
     4801 gcgtgagcca ttgcgcggcg aggtaggcca cgaaccagcc taccagcgac atcaactcga
     4861 acaccagccc gcgccacgcg ccgaccagca tcgacagcag cagcgccgcc agcatgaaga
     4921 tatcgacggc gttccaggag gcggtcatcg tgctcattca gtcgaccccg gcgagcgccg
     4981 gcgtcgcccc gatcggatca aagcgtcagt accaccgcag cgatgccagc gtccttcgag
     5041 cgggcctgca ccttttcggc ctcggcccgg gtcgcaaacg ggcccagccg cacgcggatg
     5101 cgcttgccag cgctggtctc ggccacctgg gtgtaggtct tcaggcccag cttttccacc
     5161 ttcgcgcgga gttcgcgagc cgcagccggg tcggcgaacg cgcccacctg cacgacaaaa
     5221 cgccccgtgg cctcgggagc cgatgctggg gcggcgtcgg aggtgatgtc gacagcggtt
     5281 gcggcgttcc tggccgggcg tgctgccgga gcttcggatg cggccttgga cacggccagc
     5341 ggtgcgcggg ctgatgccga cggctccgca gcaggggcag gcctggacgg caggacttcg
     5401 accttgccac tggccaccgt cgaagcagcc gccggcggtg cggacgttgc accgcttgcc
     5461 gagggctcgg atgaaggcat caggtcggca ggagactcgc gagacacgcc agttgccgcc
     5521 acatcagttc cgtcagcacc gcgggacgcg gtcgtctcgg ccggtgcggg ggtcacttcg
     5581 cgccgcggag caggcagaac gagtgcgggc gcgttgtcct gccgcggaat gtcgatcggg
     5641 atgtcgaccg ggatgggtcg cggctgcgac tcgaaaatca acgggaatcc gatcacgcct
     5701 atggtcagca gcacgacggc accgatcagc cgctggcgcg tgcgtgtgcg caccttctcg
     5761 accgcatcga aggtcggcgc cgaacgggtt gtgctggccg gggcatcgcc cggggtcttg
     5821 cgcttgaaga aactcgtcag tcccatgggt gctggttggc gcgcaggtgg aaggcagcgt
     5881 tcgcgcgtca aacgccgtgt gaactgccca ggcggggcaa gccgtgtttc agcacgccac
     5941 cgacggtcag gaacgacccg aagacaatga ttctatctgt cgggtccgcc acttcgaggg
     6001 cgtgattcag cgcttgcatg gggtcggcgt gcgtcgagac ggcgatcggt ccgggaccct
     6061 tcagggacag ttgctcatgg accgttttca ggtgctcggc ggtcgccgcg cgcgagatgg
     6121 ggaggtcggt gaagcaccag gtgtcgacga tcggggccag ccgccggaag attccggcca
     6181 ggtccttgtc gtgcatggcc ccgaacaccg catgggtgcg cggatgaaag cccattcgat
     6241 cgaggttctg cgccagtgcg gccactgcat gcgggttgtg cgcgacgtcc agcaccaggg
     6301 tcggctgacc cggaacgatc tggaagcgac cgggcaggtc gagcatcgcg aaaccctggc
     6361 gcaccgcctg cgcagtgatc ggcaaccggt caccgagggc ctcgaatacc gccagagcac
     6421 ccgacgcatt gatcagttga ttggctccgc gcaaccccgg gtaggccagg ccgttgtagc
     6481 gcttggaccg cccagcccag gcccactgct gctgatcgcc ggagtggttg aaatcgacgc
     6541 cgaaacggcg caggtcggcg ccgatgtccc tcgcacggtc aaggacgctt tgcggaggca
     6601 ccgggtcgct gacgatcacc gggcggccgg cgcgcatgat gccggccttc tcgcggccga
     6661 tcgattcgcg gtcggggccc aggaactcca tgtggtcgag gtcgatgctg gtgatcaccg
     6721 cgcaatcggc gtcgaaggcg ttgaccgcgt cgagccggcc tccgaggccg acctcgagga
     6781 tcaccagatc gagcgtggcc tgcgacagca tccgcgcgat cgccagcgtc gtgaactcga
     6841 agtaggtcag tccgatgtca ccgcgggcga gttcgaccgc ctcgaaatgc acgagcaact
     6901 ccgactcggt gaccatctcg ccattgacgc ggcagcgctc ctcgaaatgc accagatgcg
     6961 gcttcgagta gagcccaacg cggtaaccgg cacgcagcgc gatcgactcg agcatcgcgc
     7021 aggtcgaccc cttgccgttg gtcccggcca ctgcaatcac cggtacatcg aagttcagat
     7081 cgaggcgcgc gcgcacctgc tcgacacggt gcaggcccat gtcgatttcc ttcgggtgca
     7141 gcttctcgca gcgtgccagc caggccgcca gcgaggtcgg gagcggggac gacaagatgg
     7201 ttcgctgggc gcgggcccgc cgtcaggcga gcgcgtcggc gctctggcgc tgcagcatcg
     7261 cgatcatctt ggcgatggaa gtccgcagtt cccggcggtc gatgatcatg tcgaccgccc
     7321 cggtgtgcag caggaactcg gcacgctgga agccttcagg cagcttctcg cgcacggtgt
     7381 tctcgatcac gcgcggtccg gcgaagccga tcagggcctt gggttcggcg atgaccacgt
     7441 cacccatgaa cgcgaagctc gcagacacgc cgcccatggt cgggtcggcc agcacgctga
     7501 tgtagggcaa cttcgccttc gccagccggg tcagcgcggc gttggtcttg gccatctgca
     7561 tcaggctcag cagcccctcc tgcatgcgcg caccgccggt ggccgtgaag ctgatgaacg
     7621 gcgtcttctg ctcgactgcg gtttcgacac cgcggacgaa gcgctcgccg accacggaac
     7681 ccatcgaacc acccatgaac tggaactcga agcaggccgc cacgaccggg atgctgtgga
     7741 tcgcaccgcc catcacgacc agcgcatcgg tctcgccggt ggcttccagc gcttccttca
     7801 gtcgttcagg gtacttcttg ctgtccttga acttcagcgc atcgaccggc acgacttcct
     7861 gaccgatctc gtagcggcct tcagggtcga gaaaggcgtc cagccgagcc cgcgctccca
     7921 tccgatggtg atggccgcat ttcgggcaga cgttgatgtt ctgctcgagg tcggtcttgt
     7981 agagcaccgt ttcgcacgac ggacacttga tccacaggcc ttcgggcacc gagcggcgct
     8041 cgctggggtc ggtctgctgg atcttcgtcg gcaacagttt ctcgagccag ctcatgcgtt
     8101 ttctcctgtc agcgtatcga gggcagcgcg tatctcggcg atgaaacgac cagccaccgc
     8161 cgcggcttgg gcaggcgtct cgtgttcaag caactgcacc agcgccgaac cgatcacgac
     8221 cgcatcggac accgccgcca cggccttcgc ggtggccgcg ttgcggatgc cgaagcccac
     8281 cccgaccggc acgtgcacat gctcgcggat gcgcggcagc atcgattcga cggcaccgat
     8341 gtccaggtgc cccgcccccg tcacgccctt cagcgacacg tagtacacgt agccgctggc
     8401 gatgcgaccg acctccttca tccgctgctc ggtcgaggtc ggagcaagca ggaagatcgg
     8461 gtccatctgg tgactgtgca gcgtgcgggc gaacgcctcg cactcctcgg gcgggtaatc
     8521 gacaaccagc acgccgtcga ccccagccgc cttggcatcg gcgacaaaac ggtccacgcc
     8581 gtagcgctcg atcgggttgg cgtagcccat cagcaccacc ggcgtcgagt ggttgcgctc
     8641 gcggaaacga cgcacatagt cgagcacctg aggcatgccg atgcggtgca acagcgcccg
     8701 ctcggcggct ttctggatca ccggaccgtc ggccatcggg tcggagaagg gcacgccgag
     8761 ttcgatcacg tcggcgcccg catcggccat cgcccacagg atgtcgacgg tgctctcggg
     8821 gtgaggatct cccgccgtga tgaaagggat cagcgccttg cgtcgctgac tcttcaacac
     8881 ctcgaaggtg gcggcgatgc gactcatttc ggacctttca cggggctgga tccgcccttc
     8941 accgtctggc ctcgacagga aggccggcag aagaactcgg cgccggacag gtctgccacc
     9001 gtgccgatgt ccttgtcgcc ccgcccggac aggttcacca gcaggctctg gtcgctgcgc
     9061 atcgtcggcg cgatcttcat cgcataagcc agcgcatggc tcgactccag cgccgggatg
     9121 atgccctcgt tgcggcaaag caggtggaac gccttcaacg cctcgtcgtc ggtgatgccg
     9181 acgtactcgg cgcggccgat gtccttcagg taggcgtgct cgggaccgac accgggatag
     9241 tcgaggccgg cggacaccga atgggtctcg atgatctggc cgttggcgtc ctgcagcagg
     9301 taggtgcgat tgccgtgcag cacgcccggc gaaccagccg acagcgaggc cgcatggtgc
     9361 cccgtctcga tgcccagtcc ggccgcctcg acgccgatca ggcgcgtgcc ctcgtgctcg
     9421 atgtagggat agaagattcc catcgcattg ctgccgccgc ccacgcaggc gatcaccgca
     9481 tcgggttgac gtccgatcat ctccggcatc tgcaccaggc attcgtcgcc gatcacgcgc
     9541 tggaaatcgc gcaccatcat gggatacggg tgcgggcccg cgacagttcc gatgatgtag
     9601 aaggtggact cgacgttggt cacccagtcg cgcaatgcct cattgagcgc atccttcaat
     9661 gtcttgctgc ccgattcgac gggcacgacc cgggcaccga gcaggttcat ccggtagacg
     9721 ttcggcgact ggcgcagcac gtcggcgctg cccatgtaga ccacgcattc cagcccgtat
     9781 cgagcgcaga tcgttgcggt cgccacgccg tgctggccgg cgccggtctc ggcgatcact
     9841 cgcggcttgc ccatccggcg cgcaagcatc gcctgcccga tggtgttgtt gatcttgtgt
     9901 gcgccggtgt ggttgagatc ctcgcgcttc aggaagatct gcgcgccgcc caattcccgg
     9961 ctgattcgtg ccgcgtgata gatcgggctg ggccgcccga cgaagtgcgc cagttccgac
    10021 cggaactccg ccatgaactc cggatcggcc tgggcctggg catacgcctc cttcagttcg
    10081 tccagcgcat ggatcagcgt ctcggcaacg aaagtgccgc catagggacc gaaatgcccg
    10141 gtggcgtcgg gctggtggta atcgaacatc gtgggttcac tcttcagttt tcattcgctg
    10201 cgatacgggc gtctgcttca cgcacctgct cgcaaaaccg gcgcatcgcc aaggcgtcct
    10261 tgacgccctt ggcctgctcc acgccggagc tgacgtcaac ggcccagggc cggacccgaa
    10321 gaatgccttc gatcacattt gcggcatgca atccaccaga caaaacgagg ggacgggaca
    10381 cgtttcgagg gatgagcgac caatcgaaga cctttccacc gccaccgtaa ccgtcgatgt
    10441 gggcatcgag gaggatggct tgcgcatccg gatggctaag cgcgaatttt agcaaatcga
    10501 accctgcgtc catgcgcgcc gcccgcagat acggccgccc cggcgcctga caggcgcccg
    10561 gggtctcatc accatggaac tgcagcagca gttgcggcat ggccgccgtg gcctcggcga
    10621 tcagttcggc atcggcgttg acgaacaaac ccacgggcgt cacgaaggcg ggcaggcgcc
    10681 gcgccaactc tgtcgctcgc ctgacatcga cgaaacgcgg gcttttttcg tacagaacga
    10741 atccgatggc atcggcaccg gcctcgacag cgtcgtcgat gtcctgctct cgggtcaatc
    10801 cacagatctt gatgcgggtt cgtgagttca tcccgcaagt gtcagggcaa ccagtcggtt
    10861 gcgggagggc gcttcgggat gccatgccga tcgtcgtagt agggcccggt gaaatacagg
    10921 ccgtcgggcg agaaagtggg cgccgccagt tcgcgccgac gggaagccag cacctcgccc
    10981 atccacgaca ccgggcggct gccgttgccg acggctgtca ggcagccgac gatgttgcgc
    11041 accatgtggt gcaggaaggc gccggcatcg aaatcgagac gccagtaggc gccgcgccga
    11101 ctgatttcga tcgaccgcat ctgcttgacc ggcgaggcag cctggcactg cgaagagcgg
    11161 aacgccgaga aatcgtgctc gcccagcagc aaagctgccg cctgccgcat cgattcgcca
    11221 tcgagcgggc ggaatgtcca gccaacccgc cccgcttcga gcgacggacg caccggagat
    11281 tccaggatca ggtagctgta gcgtcggccg atggcgctga aacgcgcatg gaattcagag
    11341 tcgacgcact ggcaccactg gatggccacg tcggcaggca ggtaccgatt ggtgccccgc
    11401 acccaggaca cctcctctcg atcgacagga gcatcgaggt gcaccacctg attgagcgcg
    11461 tgcacgcctg catcggtgcg cccggcacag gtcacggtca tcgggagatc gacgaactgc
    11521 gagagcgctc gctcgacaca accctggacc gataccgcgc tggactggcg ttgccagccg
    11581 caataggccc ctccccggta ggaaatgccc agcgcgagtc gattcatccg ggaacgtgga
    11641 gcctcaccgc atggctgcgg tgatcaagag tcaatcgagg tccgcaagca tgccctgggc
    11701 ttttgccttc agcgtgcctt ctgccttgtt ggcgacttcc tgcagcaggt cgcgtgcacc
    11761 ctcggtatcg ccgatctgcc ggaactcctc ggccaattcg agcttgcgcg ccagcggatc
    11821 ggcgttgtcg tcgtcggctt cgtcgtcgac cacgccatcg gtctcgatct caatgtccgt
    11881 tgcgcccgct gaagcgggtg ctgcgggagc atccagatcg agcgagatcg acgacatgtc
    11941 gaattcaagg ggtgacttcg aatcggggcc cggggctgcg gccgaagggt agaacgcctg
    12001 ggctgcggac ggcgtaggcg aaggctcgaa gtcgaacgat tcgagcggtg gtggtgacgg
    12061 cggcgcaact gctgcaggag ccgactccag ggcatcgagg tcgaggtcga aatcgatttc
    12121 ctcggctgcg ccctctgacg cattgcccag cacttcggtg ggtgtctcgt tgaagctcga
    12181 ctgggttggc agcaccgact gcggcatggt gctggcaccg agcggctccg acatcagccc
    12241 tgaatcgctg gccgacatcg aatccggcga tccgccgggc tggtagagcg tgttgtcggg
    12301 gtcgatgtcg agcccgagtt cctgtgcctt ttcccaatct tcaccactgc caccggtcag
    12361 ggcgaacagt tgagtggcaa gcaactcgaa tcccttggtg tcgcgacgct tggcgtacac
    12421 ctcaagcagc ttggtgcgga tcgccatgcg ctccgggctg ctgcgcatgg cttccttgag
    12481 gatctcttct gcctgcaggt cgcgtccgta agcgaggtaa acgtcggcct cggccaccgg
    12541 atcgacgtcg ccgatggcat caagctgact caacgagtag ctcatcgacg acggcgctcc
    12601 cgaagcggcc tcgcgggtgt cgatgcgctg accgccgctg gcgccgaaga acgagtcagg
    12661 ctggaggcgg ctttcgagga atgaggtttc tccggtgtcc agcttggcgc gccgggcccg
    12721 gtagacaccg aaaccgagca acgcgaggat ggctccacca ccgacaccga gcatcaaggc
    12781 gttgccttcg aacagttcgc tgaggaaccc gggctcttcg gcctcctgcg gcgcggcggc
    12841 cttggcaggc gccggcttcg aggcagaagc cgctgcagcg gccttcttct cctcagctac
    12901 caccgtggcg ggaggctcgg ccggcatgga agccacgacc ggggcagttg caggcgctgg
    12961 tgccactgaa gcggcaggtg ccggagccgg aggtgccgca gcagcaggtg tgccgccgtt
    13021 ggcattggtg gcgccagaaa gcttcttgag ttcctcgacg ttcttcgaca gctcggccac
    13081 gcgaacggct gcgtcctgct tttccttgtc ctttgaaatc ttgacttcag gggcgcccgg
    13141 cttggcagcc gaagctccct tgctcagggt cagcttgtcg ggcgcaaccg cgttggtcgt
    13201 cttgcggtca tcgacgctcg cctgcaccgt gccggcgtcc ttgcgggccg ggacgtcggc
    13261 ctgcaccgca ggcacgccag acgcgagtcg ttgccggaag ctgccgaaat cggcgctttg
    13321 agcctggatc agctgacgcg cttctgcagc gtcgacagct gctgctgcct cggcactcgg
    13381 aacgctcagc agtgctccgg ccttcaggcg attcatgttt tcgccgacga acgccttcgg
    13441 attgccgcgg tagagcgaaa ccagggtctg gtcaagcgac acgctgccgc gacgagtgcg
    13501 cgaggcgatg ccagtcaggg tgtcaccgct tcgcacccgg taggtgtctc cgaccggctc
    13561 ggatggtgct gcgggcgtcg ccgccggcgt ggcggcccgt tcaggagctg gcgccggggc
    13621 ctgcgcgact ggggcggcag gcgcagccgc acgggccgga cgcggggcgg gtgccggcgc
    13681 ggaaatcact ggggcggtag cgaccgcgga tggcggggcc gaggctgcgg cctggggccg
    13741 atcagttggc ggatcgaaca gcagcgtgta ctcgcgcacc agccggccgg tcgaccagga
    13801 caactcgagg atgacgtcga cgaagggctc ctgcaccacc cgatcgctgg tcaatcgcag
    13861 gaacgggcga ccatcggcgc gcttgagcag cgttgcctgt gtggaaggca acacgacgtt
    13921 gtagtcgacg ccggaggcgc ggtaggtgtc cggcgctgcg atccggatct tgaggttgcc
    13981 ggcctcttcg ggcgtcaggc tcgtgacatc gatctctgcc cgcagcgtct cgccgagcga
    14041 ggactgcacc gagaggcgcc ccagccccag ggcccaagcg ttgacggcac ccagcgaaag
    14101 tgcagccacg gccacactgg acagggcaaa tcgcccgagc tgctgcgtag tgtttttcaa
    14161 ggcgtctccc aaccccattg ttcgagccgc cttgcggcac cgaccagacg acctcagccg
    14221 ctttcgaggc ggcattggtc aagaagatga atatcgataa atcacaatag catcaagcag
    14281 atacggtgac aagctaatgc atcgcctgaa aagcaaggag ccaccacatc agttccggcg
    14341 accaccgcgc tgtcgtgtca ggacaacggc gcggcaggtt tatcgacagg ctcaggcctc
    14401 gatcaacagg cgcaacatcc gacgcagcgg ttcggcagca ccccagagca actggtcacc
    14461 gatggtgaac gcgccaaggt attccggacc catagccatc ttccgcaggc gccccaccgg
    14521 gatgcccagc gtcccggtga cggcgaccgg cgtcagttgc cgaatcgtgt cttcccggtt
    14581 gtttggcacc accttgaccc aggcgttgtc gttcgagatc agttgttcga tatcggccaa
    14641 aggcacgttc ttcttgagct tgaagagtag tgactgactg tgacagcgca tggctccgac
    14701 gcgcacgcag aaaccgtcga ccggcaccgc aggctggtcg aacccatcgc cctggcccag
    14761 gatcttgttg gtctcggcac cgcccttcca ttcctcgcgg ctgacaccga acccttcgtc
    14821 atcgcggtcc ttgccgatgc cgaggtcctt gtcgatccac gggatcagcg agccagccag
    14881 cggcacgccg aactgcgccg tctccgattc ggtcaggctg cgttgcttgg ccagcaaacg
    14941 gcggtcgatc tcgagaatgg cgctcttcgg gtcatcgagc aggccgcgca cctcggcatt
    15001 aagcgtcccg aactgcgtca gcagctcgcg catgtgctgc gcaccgccgc ccgaagcggc
    15061 ctggtaagtc tgcgtgctca tccactcgac cagcccggcc ttgtagagcg cgccgacgcc
    15121 catcagcatg cagctcacgg tgcagttgcc gccgatccag ttgcgcccgc cgcgcgccag
    15181 cgcgttctgg atcaccggca ggttgaccgg atcaagcacg atgaccgcat cgctcttcat
    15241 ccgcaacgtc gaggccgcat cgatccagtg accagcccag ccggcggccc gcagcttcgg
    15301 aaacacctcg gtcgtgtaat cgccgccctg ggcggtgatg atgatgtcgc agcgcttgag
    15361 cgcatcgatg tcaaatgcgt cgcaaagcgt cgcctcgttg cgtgcctgca ccggtgcctt
    15421 gccacccgcg ttgctggtgc tgaagaacat aggctcgatc agcgggaagt caccttcctg
    15481 ctgcattcga tcgagcaaca ccgaacccac catcccgcgc catcccacca atccgaccag
    15541 cttcatcgcc ttgcctttca aacccgaatt tcttacctgt tcgacttacg ccgccgcgac
    15601 ggcagcaacg accgccgacc ccatttcgcg cgtgcccacg cgctgcgtac cttcggacca
    15661 aatgtccgcc gttcgcaagc cactctcgag cacccgcgtc accgccgctt cgatccgcgc
    15721 cgcggcttcg ggctggttca gcgaaaagcg caacatcatc gccagcgaca gaatggtcgc
    15781 cagcggattc gcgatgttct tgccggcaat gtctggagca ctgccgtgac tgggctcgta
    15841 cagcccgcga ttcgccgcgt tcaatgatgc cgacggcagc atgccgatcg acccggtcag
    15901 catcgctgct tcgtcagaga ggatgtcacc gaacatgttg ccggtgacca ccacgtcgaa
    15961 cttctttggc gccttgacca actgcatcgc ggcgttgtcg acatacatgt ggtcgagttc
    16021 cacatccgga tactgcgcat gcacctcgat catcacgtcg cgccagaact ggaaagtttc
    16081 cagcacgttg gccttgtcga cactggtcac gcgcttgctg cgcttgcggg ccgcctggaa
    16141 ggcgacgtgg gcgatgcgct cgatttccgg gcgcgaatag cgcatcgtgt cgaaggcttc
    16201 gggctggcct tcgaaggcgc catccggcga aaggcggcgg ccgcgcggct gtccgaaata
    16261 gatgtcgccg gtcagttcgc gcacgatcag gatatcgagc ccggccacca gttcgggctt
    16321 caggctcgaa gcgtgtgtca gttgcggatg acagatggcg ggtctgaagt tggcaaacaa
    16381 ctgcaggtgc ttgcgcagcc cgaggatcgc ctgttcgggt cgcagcgagc gctccagcga
    16441 gtcgaatttc cagtcgccga ccgcaccgaa cagcacggcg tcggcatcct gcgcaagctt
    16501 cagcgtcgag tcaggcagcg gatggccatg ggcctcatag gccgcaccgc ccaccagggc
    16561 cgattcggtc tcgaaccgca ggtcgagcac ctcgagcacg cgcacggcct cggccacgat
    16621 ttcagaaccg atgccgtcac cggggaggat cgcaattttc acgagtcaag gtcctttgga
    16681 tcggtctcgg gtcttgctcg cgacccggac ccagtgaatt gaaagacgac cggccccgac
    16741 ttagaggctg tgggccagcc acggcttctt gagcaggcgc tcggcctcgt aggccttgat
    16801 cttgtcggca tgacgcaagg tcaggccgat atcgtcgaag ccgttgagca ggcagtactt
    16861 gcggaacgcc tgcacctcga acggcagttc ggttccgtcg gccttgacga tcacctgccg
    16921 ctcgagatcg acggtcaact ggtagccgac gaacgccgcg acttcatcga acaggctcgc
    16981 gacctggctt tccggcagaa cgatcggcag caagccattc ttgaagcagt tgtcgaagaa
    17041 gatgtccgca aaactcgggg cgatcaccac ccggaagccg tactgatcga gcgcccaggg
    17101 cgcgtgttcg cgcgacgaac cgcaaccgaa attcttgcgg gctatcaata ccgatgcacc
    17161 gcgatagcgc gactggttca gcacgaaatc agggttcggc ctgcgcgcgg caggatcctg
    17221 acccggaaag cccgcatcaa ggtagcgcca ctcgtcgaag aggttgatcc cgaagcccga
    17281 cttcttgatc gacttcagga actgcttcgg gatgatcgcg tcggtatcga cgttctcgcg
    17341 gtccatcggg gccacaagac cttggtgcac acgaaatggg ttcatcgaat gctcacttct
    17401 tcttggcggc gtcttcgacg acttcgcccg cccgctgcac atccttgccg attccttcga
    17461 ccgtgttgca gccggtgaga aagacaacca aggcagcgag agcaaaaagg gtgcgtttca
    17521 tcgagatctt cctgggtcga aatgtagaaa aagtcgcctc tcgggcgctc aagggagggt
    17581 gggaggcggc gaccaaacca ggttcacagc aggcgtcgca cgtcgacgaa gtgcccttcg
    17641 atcgcagcag ccgccgccat cgccgggctc accaaatggg ttctgccacc ggcgccctga
    17701 cggccttcga aattgcgatt cgacgtcgat gcacaccgtt cgcccggctc gagccggtcg
    17761 gcgttcatgg cgaggcacat cgagcagccg ggctcgcgcc actcgaaccc agccgccttg
    17821 aacaccgtgt gcagcccttc ccgctcagcc tgctccttga ccagacccga accgggaacg
    17881 accagagcca gcttcacgtt cgaggcgatc ttgcggccca gtttgcgcac gaccgccgca
    17941 gcttcgcgca tgtcctcgat gcgcgaattg gtgcaagaac cgatgaagac cttgtcgatc
    18001 aggatatcgg caatcggctt gttgggtgtc agcgccatgt aggtcaatgc ccgctccatc
    18061 gcaccacgct tgttggcgtc cttctcccgc tccggatccg gcacccgatc ctcgatggac
    18121 agcaccatct ccggcgacgt tccccaggtg acctgcggac ggatcgtggc tgcatcgaat
    18181 tcgaccagcg catcgaagat cgcatcgggg tcggatttca gcgtgcgcca gtaggcgaca
    18241 gcctgatccc actcgacacc tgacggcgtg aacgggcggc ccttgacgta cgagatcgtc
    18301 gtgtcatcga cagcaatgag gccggcgcgc gcgcccgcct cgatcgccat gttgcaaacc
    18361 gtcatgcggc cttccatgct gagcgcacgg atggcgctgc cggcaaactc gatcgtgtag
    18421 ccggtgccgc cggcagtgcc gatcctgccg atgaccgcca gcaccagatc cttggcactg
    18481 caacccggcg aaagcacccc gtcgatgcgg atgaggaggt tcttggcctt cttggcaagc
    18541 agggtctgag tggccagaac gtgctcgacc tcgctggtgc cgatgccatg cgcgagcgcg
    18601 ccgaacgcgc cgtgcgtcga cgtgtgcgag tcgccgcaga cgacggtcat gcccggcagc
    18661 gtcgcgccct gctcggggcc gatcacgtgc acgatgccct gacgccgatc gttcatcttg
    18721 aactgggtga tgcccacccg gtcgcagttg gcgtccagcg tgtcgacctg cagtcgcgaa
    18781 atcggatcgg caatgccatt ggagcgatcg gtggtcggga cgttgtgatc actcacggcc
    18841 agattggctg acaacctcca gaccttgcgg ccggccagat caagcccctc gaacgcctgc
    18901 gggctggtca cttcatggac caggtgacga tcgatgtaca ggacggtcgt gccgtcgtcc
    18961 tcggtgtgaa cgacgtgctc gtcccagagt ttgtcgtaga gcgtgcggct catgggtggt
    19021 gacctatgaa aacagaaccg gtgattttag gcgcggagca tgctgcgaag cagcaaacaa
    19081 gggcgctcga ccattccaga tgcaagaaac ccggcatgga gccgggtttc tgtgcctcgg
    19141 acaacccgtg ttgtcagcgt tcgccgatcg gcttcacatc acgcgatggt gagccgttga
    19201 acaactggcg cggccggccg atcttgtact cggggtcgct gatcatctcg ttgagctggg
    19261 cgatccagcc caccgtgcga gccagagcga agatggcagt gaacaacgag accggaatgc
    19321 cgatcgcacg ctggacgatg ccggagtaga agtcgacgtt cgggtacagc ttgcgcgaaa
    19381 cgaagtagtc atcctcgagg gcgatcttct cgagcgccat cgccagcttc aggatcggat
    19441 cgttgttctt gcccatcgcg ccaagcactt cgtggcacgt ctcgcgcatc aacttggcgc
    19501 gcggatcgta gttcttgtag acacggtgcc cgaagcccat cagcttgacg ttggagttct
    19561 tgtccttgac ctgcttgatg aattcgccga tcttctcgac gccgcccatt ttctgcacgt
    19621 cttcgagcat gttgagggcg gcctcgttgg cgccaccgtg tgccggcccc cacaggcagg
    19681 caacgccggc cgcgatggca gcgaacgggt tggtgcccga cgagccgcac aggcgcaccg
    19741 tggacgtcga ggcgttctgc tcgtgatcgg cgtggaggat gaagatgcga tccatggcgc
    19801 gcaccagaac atcgttgggc acgtagcttt cgcaaggcgt ggcaaacatc atctgcatga
    19861 agttgccggc gtacgacaag tcgttcttgg ggtagatgta cggttgaccg accgagtatt
    19921 tgtaggccat cgccaccagt gtcggcatct tggcgatcag gcggatcgcc gagacttcgc
    19981 ggtgctcggg gttattgatg tcggtgctgt cgtgatagaa ggccgacaga ccgccgacca
    20041 gtccggtcag gatggccatc gggtgcgcgt cacgacggaa gccacgcagg aagaactgca
    20101 tctgctcgtt gaccatcgtg tggttcgtca cgcgcttggt gaactccacg cgctggtcgg
    20161 cgttgggcag ttcacccttc agcaacaggt agcaggtttc aaggtaatcg cacttggtgg
    20221 caagctgctc gatggggtag ccgcggtaaa gcaactcacc cttgtccccg tcgatgtatg
    20281 tgatcttgga gttgcaggac gccgtcgaca gaaagccggg gtcgtaggtg aacttgccgg
    20341 tctgcccgta gagcttgcgg atatcgatca cgtccgggcc aaccgaacct ttgaagatcg
    20401 gcaggtccat gctcgggctg ccgtcggaaa acgacaaggt ggctttgacg tcggaagggg
    20461 tcatgatcgg tccttggggt gaggctagac aggtcgtcgg ggtggataaa gcggggggtt
    20521 cagcgaggtt cccgcatcaa tcgcaggacc tccagaatgt tgtcgttgag caattcgcct
    20581 tcgggctgct gccgggccag cagcagatcg agaaggtcgt tgtccgaaag atccatcagc
    20641 accatcaggc cgtcggcctg tcgctgcgtc agggtggatt gatatcggtc aaaaaagcgc
    20701 tcgacaaaga gatcgttttc cagcaaccca cgccggcagc gccagcgcag cttgctcaat
    20761 gcacggtcat cgatcaacgc atccatggtg tgggtcgaag ttgagccgcc ttcagaccga
    20821 acggcggatc atcatttcct tgatcttgcc gatggccttc gtggggttga gccccttcgg
    20881 acagacgtcg acgcagttca tgatggtgtg gcagcggaac aggcggtacg g
//
LOCUS       DAOORV010000060        20352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3224_length_20352_cov_7.410898, whole genome shotgun sequence.
ACCESSION   DAOORV010000060 DAOORV010000000
VERSION     DAOORV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20352
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_20352_cov_7.410898"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PKV56_09685"
     CDS             complement(<1..1531)
                     /locus_tag="PKV56_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX68123.1"
                     /translation="MSASSLARLAFAFALAACALLPAGYLLLTALPILLDGGWLAHFA
                     TTSLPHQARTSLWVALEASAVAFAVGAIPAVMVSRFEFRGRHLVSLLALLPLLFAPYV
                     TASTWTVSFSSAFFEGRHALAIQHGLTCSPYVFIVFRVAAARIPNSFSELAAGLGLGP
                     WQRLRRVHLPAYAVPAAAGLMIVFAQAIGDYAAAERLGIDTLSVGVLNLWLASQSSSV
                     AAIVSVVMIVPSVLLVGVAAWASTSIISRNPVPPASAAASRKPLAATWARAIVAWSAL
                     CSLAGFWIPEALTVRWAWLTWDKTRFAAIPGDMINAAGTSLCTALVVAVICALTALLL
                     RSGGRSTLAERVPWLFLTNYFLPSLVLALAFVMMSRDGSLGAHWLGSWRDSRLLIVVS
                     EALRFLPFAMLPVLDALRRTPHATIEAARAFGAGPVRARLVAFAGHLWPALLLGCALV
                     FMESLKELDMSLTLQPFGYSSPALKIYAFSRHQNMDRAAVWVLISQALMLLPLLLCWW
                     RM"
     gene            complement(1581..2639)
                     /locus_tag="PKV56_09690"
     CDS             complement(1581..2639)
                     /locus_tag="PKV56_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX68124.1"
                     /translation="MTDLKQRWIPSLLILLGALATSVGSVAQQRELRVISDRTDQGTL
                     RPILDAFEARSGAKVIGVFMDQGLVNRLESRPTEADVVITKDAELLDIAAQRGLLDPI
                     KSAKISASIPPEFMDPAGRYFVDAYRARIIFYSRARVKPGDLSTYEDLAGPKWKGRIC
                     LRSGSHDYNLALFGQFFASFGADKAREVISGIGANLARAPKGNDREQARAIFEGKCDV
                     ALMNTYYHPLMAANAEQKAWADAVGVYFPSQAGKGTFIMRSAAGLTKATDNRDLAIGL
                     LEFIASKEGQQLMVDRTKQYSVLPDVAVHPLMVSLGAEQGLVDGKFKVDFVPLDKMAG
                     KREEVIKFVNEIRFDAER"
     gene            complement(2838..3638)
                     /locus_tag="PKV56_09695"
     CDS             complement(2838..3638)
                     /locus_tag="PKV56_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68125.1"
                     /translation="MADGITNIYDDTLDVIVVRGAFDTATLAEAGERLDSNASDPGWS
                     RPNVKMPPEDLQLLGTDTPATPTYQEPRGASLEAYLGSAASHAAAPARIFKPGFDGVS
                     EISAVLSQISGARPAQVARSSDDRPYVPFTIRRLVDGKQVGIHHDYHYRLDLYSDLSG
                     QVDTRTLISYVATLRSPEQGGALLVYGATSDEPDVPRLANGFSYDLSAVEERYDCKRF
                     VMNPGDLFLLASGRCLHRVERISGPKARITMGGFLALAKEGDRVLYWS"
     gene            4241..5620
                     /locus_tag="PKV56_09700"
     CDS             4241..5620
                     /locus_tag="PKV56_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOX68126.1"
                     /translation="MTPTLVLNVVGLTPGLLKHAPNLKGLALEGAVRPLTTVLPAVTT
                     TVQSTMLTGSLPRDHGIVANGWYFRDLSEVWLWRQSNRLVHGEKVWEAAKRRDPAFTC
                     AQLFWWYNMYASVDIAVTPRPMYLADGRKLPDIHTHPAGLRDELQGALGTFPLFQFWG
                     PGAGIASSAWIANSSLHVMKRHRPTLTLVYLPHLDYDLQRLGPNHPDIPAQVAAVDAL
                     CAPLIEEARRQGAHVVVLSEYGITEVSRPVHINRALREAGWLKVRLEQGLEQLDAGAS
                     DAFAVADHQLAHVYVARPELLPEVAACLAALPGVERVLDDAGKQAAGLNHARAGELVA
                     VAQADSWFTYYHFLDDALAPDFARTVDIHRKPGYDPVELLIDPKIAFPRLAVASRLAR
                     KAMGMRYLMDVIGLDATVIKGSHGRPTDRPEDGPLFLTTAPELIADGPVPATAVKQVL
                     LDHVFGAAA"
     gene            5617..6516
                     /locus_tag="PKV56_09705"
     CDS             5617..6516
                     /locus_tag="PKV56_09705"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HOX68127.1"
                     /translation="MNTVVNAFAVNAASTPAVVLQQWLLRRLGPAAGLWLEEALASLG
                     GGGSDRDLYRCVSLVSRKLGKAALELDAAERAAACVARPDWDPSSWTVDQAARILLLL
                     VTGPEGGRFASRLDQLCATADIDELVAFYRGLPLYPEPSRHRARAAEGVRSNMKVVFE
                     AVVHRNPYPAEQLADEAWNQMVLKALFVGSALHPIVGLDRRSNAELARMLGDYAHERW
                     AARRTISPELWRCVGPFAVGPLLEDFSRVLETGTAAERAAAVLALRNATDADAHGLLD
                     RHAAFRDVVAARGVDWTTVLAFN"
     gene            6533..7456
                     /locus_tag="PKV56_09710"
     CDS             6533..7456
                     /locus_tag="PKV56_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX68128.1"
                     /translation="MRFIDPHIHMSSRTTDDYERMKAAGVVAVIEPAFWLGQPRTNVG
                     SFIDYLSSLVGWERFRASQFGIRHYCTIGLNSKEANNEELAEAVMDILPRYLSKEGVV
                     AVGEIGFDDQTALEDKYYRAQLQLAREFDMVVMVHTPHRDKKAGTIRSMDVAVEMGMS
                     PGKVVIDHNNEETVRAVLDRGFWAGFTLYPQTKMGNERMVEIVRQYGSERIFINSSAD
                     WGVSDPLAVPKTGRLMLERGIALEDVEATCYGNALRVYGQSGQFDEAHWMTPEAVDQR
                     SLFEGNSVLRGQTPRVDDAPAPAVWAEDVTK"
     gene            7496..8707
                     /locus_tag="PKV56_09715"
     CDS             7496..8707
                     /locus_tag="PKV56_09715"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOX68129.1"
                     /translation="MSAQITPLPARGEERSAVYLQSFAVHYEYPVYFTEHLFARDNLI
                     LRDTLLRREPSRRHRFVAFIDSNVAASFPSLAHDIAGYAVVHADAMELVAHPEVVPGG
                     EHVKNDPALLTRLQRRLVELGVDRHAFVVGIGGGAFLDLIGYVASSTHRGIRHIRVPT
                     TVLAQNDSGVGVKNGVNAFGVKNLLGSFAPPFAVLNDSDFLRTLQPRDKIAGVAEAVK
                     VALIRDRLFFEWLEASADALRACEGAAMSRMIRRCAELHMRQIAHGGDPFETGSARPL
                     DYGHWSAHKLEALTAHELRHGEAVAIGLALDTRYSVQVGMLAAGAESRVHALLKRLGF
                     HLWHPALESRDAEGQWQLLRGLQEFREHLGGDLTVTLLRDIGVGEEVHQMDSQEILRS
                     LVWLRRMEQGA"
     gene            8704..9996
                     /gene="eboE"
                     /locus_tag="PKV56_09720"
     CDS             8704..9996
                     /gene="eboE"
                     /locus_tag="PKV56_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HOX68130.1"
                     /translation="MNLGDGVHLTYCTNVHPGESWAEVRSNFDRYVLAVRDRLGVPGD
                     FGVGLRLSARAAAELSEPAALSEFRNFLADHGLYVFTINGFPYGTFHGERVKEGVYLP
                     DWREPERLRYTDLLADLLADLLPQAGPAGMPIEGSVSTVPGAFKPALGGPLEVARMVE
                     HLLLHVAHLVALRSRTGRLIALALEPEPHCFLETVAEAIDFFKLELHGPAAVRRTMEL
                     TGLDQFEAAQALHDHLGLCFDLCHGAVEFESAEECVRALDQAGIRVLKMQISAGLRLP
                     TLDAVAIEELKRFDDPVYLHQVVQQDAFGLTRFADLPEALASLDDSTAAREWRVHFHV
                     PIFLDRLAPFASTQSFVREALAMQRARPISSHLEVETYTWDVLPEPYRSGRIDESVAR
                     ELAWVRAELAPQDSVGTSPVPSAGSWRQDRSFSSEGRP"
     gene            9993..10865
                     /locus_tag="PKV56_09725"
     CDS             9993..10865
                     /locus_tag="PKV56_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOX68131.1"
                     /translation="MTPSVALRLGRVSNLPTVWTNALVGVLLAGGFLTDPRLPMLLLA
                     LSLFYVGGMFLNDAFDREFDAKHRPERPIPAGLVTAREVFTCGFGMLVVGTAIVAVAS
                     RGPDGMPAWRAVASCAALSGAIVFYNAHHKGNPLSPLLMGLCRVFVVLTAAFSLAAAL
                     PQAVFLAVIALLCHLIGLTYIAKQEHLDRIGSLWPLGFLAIPVIYGVVLAVSAPATWL
                     PLLLYAGVVWFALSLLRRRARGDVPRAVVTLIACMSVLDGVVLAGSGHLWPAGLAILA
                     FLLTLVLQRWVSGT"
     gene            10878..11180
                     /locus_tag="PKV56_09730"
     CDS             10878..11180
                     /locus_tag="PKV56_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOX68132.1"
                     /translation="MTEQLAADCPGAPGRVAPQIDRNRCEAKGPCVEVCPFNVLEIRS
                     LDASDRESLSWRGRIKAWAHGNRQAHVIRPADCRACQLCVNACPEAAIRLVPYVPA"
     gene            complement(11344..12051)
                     /locus_tag="PKV56_09735"
     CDS             complement(11344..12051)
                     /locus_tag="PKV56_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOX68133.1"
                     /translation="MSAMSSVRPDHPIRTTADDPAATLRPGAGKRKSALLHPAAKPTL
                     FALCLLPFAWLLYGAWADTLGANPAEALIRSTGDWTLRFLCATLAITPLRQWFEAPAL
                     ARFRRMLGLFTFFYVVMHFLSYAWLDQSFDLDAILKDIAKRPFILVGTTGLLLMAPLA
                     ATSFNRAIKAMGAARWQMLHRTIYGIALLGLLHFFWMRAAKNDFAEVAMYAAIVCVLL
                     GYRVKRWNSQRSLAARA"
     gene            complement(12074..13069)
                     /gene="msrP"
                     /locus_tag="PKV56_09740"
     CDS             complement(12074..13069)
                     /gene="msrP"
                     /locus_tag="PKV56_09740"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOX68134.1"
                     /translation="MHFHEDQGFVHRVASEITPRDVYDQRRTLLKALAAGSGGLALAA
                     WAGRDALAQTSARPGKLAALPSARTTTPGGMTLEKLTPYPDVTSYNNFYEFGTDKSDP
                     ARHAGALKTRPWTVSVEGEIRKPRIYDIEELLKLGAMEERIYRMRCVEGWSMVIPWIG
                     YSLSELIKRVEPTGNAKYVEFVTLADQKMMPGVGSRVLQWPYVEGLRLDEAMNPLTLL
                     TFGLYGEVLPNQSGAPVRAVVPWKYGFKSGKSIVAIRFVEKEPRTSWNKAASSEYGFY
                     SNVNPAVDHPRWSQASERRIGEDGLFQKKRATLMFNGYEPQVGQMYAGMDLKKFF"
     gene            complement(13245..13481)
                     /locus_tag="PKV56_09745"
     CDS             complement(13245..13481)
                     /locus_tag="PKV56_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68135.1"
                     /translation="MQSFVGRPPEGDGFSRLDADFVRVIEDVIDTLIVKNVLNITDLP
                     AEAQAKLFARKSFRERSTRNSLHLFSASGADEVI"
     gene            complement(13742..14359)
                     /locus_tag="PKV56_09750"
     CDS             complement(13742..14359)
                     /locus_tag="PKV56_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX68136.1"
                     /translation="MKFASALLSCFLLAAFTASANESPAPAKPDLAKGAATAAVCSAC
                     HSSDGSRGSPAQPILQGQHPEYLVKQLTEFKKGVRANAIMSGMSAPLSDSDMKNVAAF
                     YASKKAEPGVAQNAELAALGEKIYRGGIAERSIPACAGCHSPNGAGIPSQYPRLAGQH
                     AEYTEAQLIAFRGGVRKNSAQMTGVAAKMNDQEIKAISDYIAGLH"
     gene            14423..15175
                     /gene="yihA"
                     /locus_tag="PKV56_09755"
     CDS             14423..15175
                     /gene="yihA"
                     /locus_tag="PKV56_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOX68137.1"
                     /translation="MIEQTKVLPSAGNAITEALSAEKRALAWTHTARFLTTASQLREL
                     PQTELPEIAFVGRSNAGKSTAINTMTQQKRLAFASKTPGRTQHINLFELGPKTRADAL
                     FADLPGYGYAAVARGAKLRWQQVMADYLEVRRSLAGLVLMIDSRLGFTDLDRQLLGFV
                     GPRIGNGSVKLLVLLTKADKLNRSEAAAALVAAQEALGEMTTPEADVNVALFSALKKS
                     GVGDAATLIHGWVQAGQRPGEPSGATEEQVQP"
     gene            complement(15143..17410)
                     /locus_tag="PKV56_09760"
     CDS             complement(15143..17410)
                     /locus_tag="PKV56_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOX68138.1"
                     /translation="MHLPSVVLSACAFIGLGFAVAASAAEPVSPAERALARPRIGLVL
                     SGGGARGLAHVGVLKLLERERIPIDVIAGTSMGAIIGGLYASGMDANRLESELLAVDW
                     DEVFASRVDRPLLTQRRKEADFELSPLLEFGMRDGELRAPLGALSGRGLETLLRRYTL
                     PVRSVRDFSRLAIPFRAVATNLETGEAVILREGDLALALRSSMSVPGVFAPTEADGRI
                     LGDGGLVNNLPIDVARAMGADIVIAVNIGTPLASREALGSVGGLTTQMISILTEQNVQ
                     RSLATLQPRDVLIAPTLGVLTSGDFGKTRELIDRGEAGGQPQLQRLAELSLSPPDYAR
                     WQAAHRQGEAQSAQLAFIRIEGSKLTNPQRLLDQLESAGGAVFDDAKAERDARRLAAS
                     GDYTRADYRLIRTPDGDGLVFDLEDKPWGPHYFRVGIDLFTDFRGNSAFNVKLNHNRR
                     WLDEAGTEWRNFAQIGETPKLLTELYHPLNWSIGLQHDWFVAGYASIERRKITYYEPD
                     SGDEQGRFTRTSARLGIDLGQPWGEFGEVRLGVSHVVFRNQPDLVSSDFNGPRNALTV
                     HETGLRVSATVDQLDFVNFPLHGYRVVGEAVVGARSVSGPQGDEQFTRLEFEGTGVTT
                     WGRTTFNAHMSAKSAGGEDLSGLGRYNYNLGGFHRLSGYRYNQLAGSHVLFGRLTMYQ
                     RLEDIPLFTRGMFIGGSMEAGNAWIEAKDISLSRLRAGFSGFVGADTGVGPLYLGVAY
                     APRGDLGLYLFLGRP"
     gene            complement(17452..19668)
                     /locus_tag="PKV56_09765"
     CDS             complement(17452..19668)
                     /locus_tag="PKV56_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOX68139.1"
                     /translation="MPVSQLMLRFVDDELSRSSALIERTVTTTLAHLQRAAKPGAPAT
                     ASERHVQRESVDSLQQGQRRFAQTFIDGLSRTVTAEIQGLDSGTSTEHVTAIDGLQLM
                     DETRVEADIEISRASQLIDSIAEWELRELQTFTSALTGQTHVSADSNPLRPAAYAQAL
                     WEAVCAINDTPAQRSLLLRTAASSMAAELKMAWAGACTRLESQGVEPSIYRTMVLPSG
                     LQTAATSRNNSSEQQGFEQLLNRMPGSQGGGAPAGSDTGAVTLRTLGPSADSPSAAAK
                     THDSSRLTAAFEQALQQIELLLQRRAESGGAGAEPPPMLDAAPLREHRAALLAHAQEV
                     VDRQIVELLSRIFESVLSDTQLSAAVRAVMARLQVSALRVALVDRSMLAGHQHPVWAL
                     MNRIASASQVWPQPGDVRAHRLTTLCESLAHEIAGAANQTASLYAEGLQRLESHLAGE
                     LRQQQEQAQSTIEALKATERREVLERELSHQLAEQIRSVHTTPRIRNFLVQSWACVVT
                     ESMLRYGDADERTAGYLKAVDDLLWSLRLPDHPQSRQRLLALLPTLLQRLRSGMALIA
                     LPDADQQAVLDELMAIHTEALRPGARADTREPTPQEIMKRLREEEPAEPLVQAGKGFA
                     DSLIDVFSMDTIPADLMSDGDTPPSGTKNPVDAMVPGSAFRWFLSGRWRRVQLLWRSD
                     SQRFFVFAGETAGRTHSITRQALELLTAEGLVKPLSDSELIQRAIERVRNQLGEIG"
     gene            complement(19829..>20352)
                     /locus_tag="PKV56_09770"
     CDS             complement(19829..>20352)
                     /locus_tag="PKV56_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOX68140.1"
                     /translation="AVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVAGGGVALL
                     RAKQLAGEIKGDNPDQDAGIKLVLKAIESPLREIVYNAGGEASVVVNAVLAGKGNYGF
                     NAANDTYGDMIEMGILDPTKVTRTALQNAASVASLMLTTEAMVAEAPKEESAGGGMPG
                     GMGGMGGMGGMDM"
BASE COUNT         3389 a         6556 c         6713 g         3694 t
ORIGIN      
        1 ccatgcgcca ccagcacagc agcagtggca gcagcatcaa ggcctgggat atcagcaccc
       61 acacggcggc gcggtccatg ttctggtgcc tggagaaggc gtagatcttc agcgctggcg
      121 acgagtagcc gaagggctgc agggtcaggc tcatgtcgag ctccttgagc gactccatga
      181 acaccagcgc gcagcccaga agcagggctg gccagaggtg tccggcgaag gccaccaggc
      241 gcgcccgcac cggccccgcg ccgaacgcgc gtgccgcctc gatggtggcg tgcggtgtgc
      301 ggcgcagtgc atcgagcacg ggcagcatgg caaacggcag gaatcgcaac gcctccgaca
      361 ccacgatcag cagtcggctg tcgcgccatg agccgagcca gtgtgctccc agggatccgt
      421 cgcgactcat catcacgaaa gccagtgcca gcaccagcga gggcaggaag tagttggtca
      481 ggaacagcca gggcacgcgc tcggccaggg tggatcgccc accggatctc agcagcaggg
      541 cggtcagcgc gcagatcacc gccaccacga gtgccgtgca cagcgaagtg ccggccgcgt
      601 tgatcatgtc ccctggaatg gcggcaaacc gggtcttgtc ccatgtgagc caggcccagc
      661 gcacggtgag tgcctcggga atccagaatc cggccagcga gcacagcgca ctccacgcca
      721 cgatcgcccg ggcccaggtt gccgccagcg gcttgcggga ggccgccgcc gacgcgggcg
      781 ggacggggtt gcggctgatg atcgaggtgg atgcccaggc ggccacgccc accagcagaa
      841 ccgacggcac gatcatcacc accgaaacga tcgccgccac cgatgagctc tggcttgcca
      901 accagaggtt gagcacgccc acgctcaggg tgtcgatacc caggcgctcg gccgccgcgt
      961 agtcgccgat ggcctgggcg aacacgatca tcagcccggc cgcagccggc accgcatagg
     1021 ctggcaggtg cacgcgccgc agtcgctgcc aggggccgag gcccaggccc gcggcgagtt
     1081 ccgagaacga gttcgggatg cgtgccgcgg ccacgcggaa aacgatgaac acgtacggtg
     1141 aacaggtgag tccgtgctgg atggcaagcg catggcgacc ctcgaagaac gcggacgaga
     1201 acgacacggt ccaggtggaa gcggtcacgt agggggcgaa gagcagcggc aacagggcca
     1261 gcaggctgac caggtgccgg ccgcgaaatt cgaaacgcga caccatcacg gccggaatgg
     1321 cgccaacggc gaaggccacg gccgatgctt ccagcgccac ccacaggctc gtgcgcgcct
     1381 gatgcggcag gctcgtcgtg gcgaaatggg ccagccaccc gccatcgagc aggatcggaa
     1441 gtgccgtcag cagcaggtag cccgccggaa gcagggcgca agccgccagc gcgaacgcga
     1501 atgccagccg tgccaggctg ctggcgctca tctcggcctg cagcctggcg gcctgcgcgc
     1561 ggcaacgcag gacagtcgca tcagcgctca gcgtcgaaac gaatctcgtt gacgaacttg
     1621 atgacttctt cgcgtttgcc tgccatcttg tcgagcggca cgaagtcgac cttgaacttg
     1681 ccgtccacca gtccttgttc cgcaccgagg ctgaccatca gcggatgcac cgcgacatcg
     1741 ggcagcaccg agtactgctt ggtgcggtcc accatcaact gctgtccctc cttggaggcg
     1801 atgaattcca gcaggccgat ggccaggtcg cggttgtctg tcgccttggt caggcccgca
     1861 gccgagcgca tgatgaaggt gcccttgcct gcctggctgg ggaagtacac cccgacggca
     1921 tccgcccagg ccttctgctc ggcattcgcg gccatcaacg ggtggtagta ggtgttcatc
     1981 agcgccacgt cgcacttgcc ctcgaagatc gcgcgggcct gttcgcggtc gttgcctttc
     2041 ggggcgcggg ccaggttcgc cccgatgcct gaaatcacct cgcgtgcctt gtcggcgccg
     2101 aaggaggcga agaactggcc gaacagcgcc aggttgtagt catggctgcc cgagcgcagg
     2161 cagatgcggc ccttccactt cgggccggcc aggtcctcgt aggtggacag gtcgccgggc
     2221 ttgacccgcg ccctggagta gaaaatgatg cgcgcccggt aggcgtcgac gaagtaccgt
     2281 ccggcgggat ccatgaattc cggcgggatg ctcgcgctga tcttggcgga cttgatcgga
     2341 tcgagcaggc cgcgctgcgc ggcgatgtcc agcaactcgg cgtccttggt gatcaccacg
     2401 tcggcctcgg tcgggcggga ttcgagccgg ttcaccaggc cttggtccat gaagacgccg
     2461 atcaccttgg ctccgctgcg agcctcgaag gcatccagga tgggccgcag cgtgccctga
     2521 tcggtgcgat cggagatgac gcgcaactcg cgttgctgcg cgacggagcc gacagaagtt
     2581 gccaatgcgc cgagcagtat cagcaggctc ggaatccatc gttgtttcaa atcagtcatg
     2641 ttcgacctcg aaggggtgca aggttgagga atggcgagga gggtggggca aggcaagtgc
     2701 cgtctgttca agcagtgtgc gccgcctgaa tgtcaatcac gggtcaagtt ttgcacgacc
     2761 tgggcaactc acgggcaccc agccccctgg gtcgggggag cccccccagg tcgaccgccg
     2821 tggcacatcg ctgcgagtca gctccagtac aagacccggt cgccctcttt ggccaacgcc
     2881 agaaaccccc ccatcgtgat ccgagccttc ggacccgaga tccgttcgac gcggtgaagg
     2941 caacgaccgg aggcaagcag gaacagatcc cccgggttca tcacaaagcg cttgcagtca
     3001 tatcgttctt cgacggccga gaggtcgtag gagaacccat ttgcgagcct gggcacgtcg
     3061 ggctcgtccg aggtggcgcc gtacaccaga agagctccgc cctgttccgg ggagcgcaag
     3121 gtggccacgt aactgatcag cgtgcgtgta tcgacctgcc ccgacaggtc tgaatacaga
     3181 tcgagtcggt agtggtagtc atggtggatc ccgacctgct tgccatctac aagtcggcgt
     3241 atcgtgaatg gaacgtaagg ccgatcgtcg gacgagcgcg cgacctgcgc gggccgtgct
     3301 cccgagattt ggctcagcac ggcgctgatt tcgctcacgc catcgaagcc aggcttgaaa
     3361 atccgcgccg gggcggccgc gtggctggcc gcgctgccga gataagcctc gagcgatgcg
     3421 ccccgaggct cctgatatgt cggcgtggct ggtgtgtcgg tgccgaggag ttgaagatct
     3481 tctggcggca tcttcacgtt ggggcgggac cagcctgggt cgcttgcgtt cgaatcgagc
     3541 cgctctccgg cctcggccaa cgtcgccgtg tcgaacgctc cacgcaccac gatcacgtcg
     3601 agcgtgtcgt cgtagatgtt cgtgatgcca tccgccacgc ccgaaaggtt gcgggcatcg
     3661 acgtcaaccg accgcaccat cctgggggga tgctgcgcga cctgatctga cgttccggac
     3721 atcggaggag actagggtcg cttcctgccg gagtcaatgt gtgacaacaa cgaagggcgg
     3781 cagtcccgat atgcgcgcgt tgttgtctcg tgggctttgc tgaagcacgt aagcatgtgc
     3841 tgactgtcac ggtccgcccc gctccccctg acctggaggg ggtgtcaagg aaacgccgac
     3901 cgctacactc gtttgaaggg gtgcattggt tcccttgagg gtttttttcg gcgtgttgtt
     3961 ccgcagttgt ctgcaccgat attcaacggg ctcaggctgt gagttcgctg cggcctccgg
     4021 ttgtgaattg cacgaaactg cgtcatcgga ccctgaaacg gaaggggagc acgaagttgt
     4081 accaggcatc caacgccgcc ccgtccaggc accccgcggc ccccgattcg ggacaggcag
     4141 gggcaggcgg cggctcctct gtggcgtccg ggccagttgc aatgcgcggg cggcggggcg
     4201 ttcgcccgct ggttgtgctc gcaatcattc aggtttcgaa gtgacgccga ccttggttct
     4261 caatgtcgtc gggctcacgc ccggcctgct gaagcatgcg ccgaacctga agggtctggc
     4321 gctcgaaggc gcagtgcgtc cgctgaccac ggtgctgcct gcagtgacca cgacagtgca
     4381 gtcgacgatg ctcaccggat cgctgcctcg cgatcacggc atcgtggcca atggctggta
     4441 cttccgcgat ctgtccgagg tatggctgtg gaggcagtcg aaccgactcg tgcacggcga
     4501 gaaggtctgg gaagccgcga agcgccggga tccggccttc acctgcgcgc agcttttctg
     4561 gtggtacaac atgtacgcct cggtcgacat tgcagtgacg cctcggccga tgtacctggc
     4621 cgatggccgc aagcttccgg atatccacac tcacccggcc gggttgcgcg acgagttgca
     4681 gggcgccttg ggtacgtttc cgctgttcca gttctggggg ccgggtgccg gtatcgcatc
     4741 cagcgcgtgg atcgcgaaca gttcgctgca cgtgatgaag cgccaccggc cgactctcac
     4801 gctcgtctat cttccgcacc tggactacga cctccagaga ctggggccga accaccccga
     4861 cattccggcc caggtcgcgg cggtcgatgc gctgtgtgcg ccgctgatcg aggaggcgcg
     4921 ccggcagggt gcgcatgtgg tggttctttc cgagtacggc atcaccgagg tttcgcgccc
     4981 ggtccacatc aaccgcgcgc tgcgcgaggc cggctggctc aaggtgcggc tggaacaggg
     5041 gcttgaacag ctcgatgccg gtgcctccga cgccttcgcc gtggccgacc accagttggc
     5101 gcatgtctac gtcgcgcgcc ccgagctgct gccagaggtt gcagcctgcc tggccgccct
     5161 gcccggcgtg gagcgggtgc tcgacgacgc cggcaagcag gctgccggcc tgaaccacgc
     5221 gcgcgcgggc gaactggtag ccgtcgccca ggccgacagc tggttcacct actaccactt
     5281 cctcgatgac gcgcttgcgc ctgacttcgc gcgcaccgtc gacatccacc gcaagccggg
     5341 ctacgacccg gtcgaactgc tcatcgatcc gaagatcgcc ttccccaggc ttgctgtggc
     5401 ctcgcggctg gcccgcaagg cgatgggcat gcgatacctg atggacgtga tcgggctgga
     5461 cgcaacggtc atcaagggct cgcatggtcg gcccaccgat cgcccggaag acggccccct
     5521 gttcctgacc acggcacctg aactgattgc cgacgggccg gtgcccgcca cggcggtcaa
     5581 gcaggtgctg ctggaccatg tcttcggtgc cgctgcatga ataccgtcgt caatgcattc
     5641 gcagtgaacg cggcgtcgac gcctgccgtc gtgttgcagc aatggctgct gcgtcgcctg
     5701 ggaccggctg ccggcctctg gctggaggag gccctcgcca gtctgggcgg cggcggaagt
     5761 gaccgcgacc tgtaccgctg cgtgagtctg gtctcgcgca agctcgggaa ggcggcgctg
     5821 gagctggatg cagcggagcg cgccgcagcg tgtgttgcgc ggcccgactg ggacccttca
     5881 tcgtggacgg tggatcaggc cgcacgcatc cttctgctgc tcgtcaccgg gccggaaggc
     5941 gggcgctttg catcacgtct ggaccaactc tgcgcgactg ccgacatcga cgaactggtg
     6001 gccttctatc gcgggcttcc gctctatccg gagccgtccc gccatcgggc ccgtgccgcc
     6061 gagggtgtgc ggtcgaacat gaaggtggtg ttcgaagcgg tggtccaccg caatccctat
     6121 cctgccgagc aactggcaga cgaggcctgg aaccagatgg tgctcaaggc gctcttcgtg
     6181 ggcagcgcgc tgcatccgat cgtcggtctg gatcgccggt ccaatgcgga gttggctcgc
     6241 atgctgggcg actacgccca tgaacgctgg gccgcccgtc gaacgatcag cccggagctc
     6301 tggcgttgcg tgggaccgtt cgcggttggc cccctgctcg aagacttctc gcgcgtgctc
     6361 gaaaccggta ccgccgccga gcgcgccgcc gcggtgctgg ccttgcgcaa tgccacagat
     6421 gccgatgccc atggactgct tgatcgtcat gcagcatttc gcgatgtcgt cgccgcgcgt
     6481 ggcgtcgact ggaccacggt cctcgccttc aactgattcc agggagcaag ccatgcgctt
     6541 catcgacccg cacatccaca tgtcgtctcg cacgaccgat gactacgaac gcatgaaggc
     6601 cgcgggcgtg gtcgccgtca tcgaaccggc cttctggctg ggtcagccgc gcacgaatgt
     6661 cggcagcttc atcgattacc tgtcgagcct ggtcggctgg gagcgctttc gcgcttcgca
     6721 gttcggcatc cgccactact gcaccatcgg cctcaacagc aaggaggcca acaacgagga
     6781 actggccgag gcggtgatgg acatcctgcc gcgctacctg tccaaggagg gcgtggtggc
     6841 cgtgggcgag atcggcttcg acgaccagac tgcgctggag gacaagtact accgtgcgca
     6901 gttgcagctg gcacgcgagt tcgacatggt cgtcatggtc cacacgccgc atcgggacaa
     6961 gaaggcaggc accatccgaa gcatggatgt ggctgtcgag atgggcatgt ctccgggcaa
     7021 ggtggtgatc gaccacaaca acgaggagac cgtgcgggcc gtgctcgacc gcggtttctg
     7081 ggccggcttc acgctctacc cacagaccaa gatgggcaac gagcgcatgg tcgagatcgt
     7141 gcgccagtat ggttcggagc gcatcttcat caactccagc gccgactggg gcgtctcgga
     7201 cccgctggcg gtgcccaaga cggggcggct gatgctggag cgcggcattg cgctcgagga
     7261 cgtggaagcc acctgctacg gcaatgcact gcgggtctac gggcagagcg gtcaatttga
     7321 cgaggcccac tggatgacgc cagaggccgt cgaccagcga tcacttttcg aaggcaactc
     7381 cgtgctgcgc gggcagacgc cgcgcgtgga cgacgcgccg gcgccggctg tttgggccga
     7441 ggacgtcacc aagtaacgcc gcctggccta tttctcgtac aggagtaccc attccgtgag
     7501 cgcacagatc acccccctgc ccgcgcgtgg cgaagagcgt tcggccgtct acctgcagtc
     7561 gttcgcggtt cactacgagt acccggtgta cttcaccgag cacctgttcg cgcgtgacaa
     7621 cctgatcctg cgagataccc tgctgcggcg cgagccttcc cggcgccatc gtttcgtggc
     7681 tttcatcgac tccaacgtcg ctgcatcctt tccatcgctt gcccatgaca tcgcaggcta
     7741 tgcggtggtc cacgccgatg ccatggagtt ggtggcgcat cccgaagtgg ttcccggcgg
     7801 cgaacatgtc aagaatgatc cggccttgct gacccgcctg cagcgccggc tggtggaact
     7861 cggcgtggac cggcatgcgt tcgtggtcgg aatcggtggt ggtgccttcc ttgacctcat
     7921 aggctacgtg gcttcatcca cgcatcgagg gatccggcac atccgcgttc cgaccaccgt
     7981 gctcgcgcag aacgactcgg gcgtgggcgt caagaacggg gtcaatgcct tcggcgtgaa
     8041 gaacctgctc ggcagtttcg ccccgccgtt tgcggtgctc aacgattcag acttcctgcg
     8101 cacgttgcag cctcgcgaca agattgccgg ggtcgccgag gcggtgaagg tggcgctgat
     8161 acgagaccgg ctgttcttcg agtggctcga agccagtgcg gacgcgctgc gtgcctgcga
     8221 gggcgcagcg atgagccgca tgatccggcg ctgcgcggaa ttgcacatgc ggcagatcgc
     8281 gcatggcggt gatccgttcg agacggggag cgcccgcccg ctggactacg ggcattggtc
     8341 cgcgcacaag ctggaggccc tgactgctca cgagttgcga cacggcgagg cggttgccat
     8401 cggtctggcg ctcgacaccc gctattcggt acaggtaggc atgcttgcgg ccggtgccga
     8461 aagccgggtg cacgcgctgc tcaagcgcct cggcttccac ctctggcacc ctgcgctgga
     8521 gtctcgggat gccgagggtc aatggcagct tctgcgtgga ctgcaggagt ttcgcgagca
     8581 tcttggcgga gacctcacgg tcacgctgct gcgtgacatc ggcgtgggtg aagaggtgca
     8641 ccagatggac tcgcaggaaa tcctgcgttc gctggtctgg ctgagacgga tggagcaggg
     8701 cgcgtgaatc tgggcgacgg tgtccatctc acctattgca ccaatgttca tccgggagag
     8761 tcttgggccg aagtccgttc gaacttcgat cgatatgtgc ttgcggtgcg tgatcgcctt
     8821 ggagtcccgg gtgatttcgg tgtgggcctg cgcttgtccg cccgcgctgc ggccgaactg
     8881 tccgaacccg cggccctgtc cgaattcagg aacttcctcg ccgaccacgg tctgtatgtg
     8941 ttcaccatca acgggtttcc ctacgggacg ttccatggcg agcgggtgaa ggagggggtg
     9001 tatctgccgg attggcgcga accggagcgg ttgcgctaca ccgacttgct ggccgacttg
     9061 ctggccgatt tgctgccgca ggccggtccg gcagggatgc ccatcgaggg cagcgtgagc
     9121 accgttcccg gcgcattcaa gccggcgctg ggcgggccgc tggaggtggc ccggatggtc
     9181 gagcacctgt tgctgcatgt ggcgcacctc gtggcgctgc gctctcgaac gggccgcctc
     9241 atcgcgctcg ctctcgaacc cgagccccac tgctttctgg agaccgtcgc cgaggcgatc
     9301 gatttcttca agcttgaact gcatggcccc gccgctgtcc gccgcaccat ggaactcacc
     9361 ggcctcgatc agttcgaggc tgcgcaggcc ctgcacgatc acctcggcct gtgcttcgac
     9421 ctgtgtcatg gggcggtgga attcgagagt gccgaggaat gcgtgcgcgc gctcgaccag
     9481 gcgggaatcc gcgtactcaa gatgcagatc agtgccggac tgcggctgcc cacgctcgat
     9541 gccgttgcga tcgaggaact gaagcgcttt gacgacccgg tctacctgca tcaggtcgtg
     9601 cagcaggatg ccttcggact cacgcgtttc gccgatcttc ccgaagcgct cgcatctctc
     9661 gacgactcga cggctgcacg agaatggcgc gtgcatttcc atgtcccgat attcctggat
     9721 cgtctggcgc cgttcgcctc gacccaatcc ttcgtccggg aggcgcttgc gatgcagcga
     9781 gcccgaccga tctcgtcgca tctcgaggtc gagacctaca cgtgggacgt tctccccgag
     9841 ccgtatcgca gcgggaggat agacgagtcg gtcgcccgtg aactcgcgtg ggtgcgggcc
     9901 gaactcgcgc cgcaggacag cgtcggcaca tcgccggtcc cgtctgccgg ctcgtggcgg
     9961 caggaccgat ctttttcgag cgagggacgg ccttgactcc gtccgttgcg ctgcgcctgg
    10021 ggcgtgtctc gaatctgccc accgtctgga ccaacgccct ggtcggggtt ctgcttgccg
    10081 gcggattcct caccgatccg cgcctgccga tgctgctgct ggcgctctcg cttttctatg
    10141 tcggcgggat gttcctcaac gacgccttcg atcgggagtt cgacgcgaag catcgtccgg
    10201 agcgcccgat tccagccggt ctggtgactg cacgcgaggt cttcacttgc gggttcggga
    10261 tgctggtcgt tggcactgca atcgtcgcag tcgccagtcg cgggccggat ggcatgccag
    10321 cctggcgcgc cgtcgcttcg tgcgctgcgc tgtccggggc gatcgtcttc tacaacgcgc
    10381 accacaaggg caatccgctg agtccgctcc tcatgggtct ttgccgcgtc ttcgtggttc
    10441 tcacggccgc gttcagcctg gccgcggcgt tgccccaggc ggtgttcctg gccgtgatcg
    10501 cactgctgtg ccacctcatc ggtctcacgt acatcgccaa gcaggaacac ctggatcgca
    10561 tcggcagcct ttggccactg ggtttcctcg ccatcccggt gatttacggc gtggtactgg
    10621 cggtgtccgc accggccacc tggctgccct tgctgctgta cgcgggtgtg gtctggttcg
    10681 ccttgagcct cttgaggcgc cgagctcgag gcgacgtgcc gcgagccgtc gtgacgttaa
    10741 tcgcctgcat gtccgtgctc gatggcgtgg tgctcgcggg aagcgggcat ctctggccag
    10801 ccgggctcgc catccttgcg ttcttgctca cgcttgttct gcaaagatgg gtgtctggaa
    10861 cctgactgga agaatgcatg acagaacaac ttgcagccga ttgcccgggt gctcccggcc
    10921 gtgtggcgcc acagatcgat cgcaaccgtt gcgaagccaa ggggccctgc gtcgaggttt
    10981 gccccttcaa cgtgctggag atcaggagcc tggatgcgtc cgatcgtgag tcgctctcat
    11041 ggcgaggtcg gatcaaggcg tgggctcatg gaaatcgcca ggctcacgtg attcgcccgg
    11101 ccgattgccg tgcttgccag ctgtgcgtca atgcctgtcc ggaggccgcc attcgcctcg
    11161 tgccttacgt gcctgcctga gttgcgggcc ttcgcacaag ctcgactcac gctggagagc
    11221 cagcttcccc gatgactgga gggcgccggc tggcgccccc ggctctttgc aaggtcgaac
    11281 ccatcttccg cgagacaccc aggcggttcg atcgggacaa cgccatgcat ccgtcgacgt
    11341 gcctcaggcg cgagccgcca gagatcgctg cgagttccag cgcttgaccc tgtatccgag
    11401 caacacgcag acgatggcgg cgtacatcgc cacctcggcg aaatcgttct tcgccgcacg
    11461 catccagaag aagtgcagca ggcccagcag cgcgatgccg tagatcgtcc ggtgcagcat
    11521 ctgccagcgc gccgcaccca tcgccttgat cgcgcggttg aacgatgtgg cggcaagcgg
    11581 cgccatcagc aacaggccgg tggtgcccac caggatgaag gggcgcttgg caatgtcctt
    11641 caggatggcg tcgaggtcga agctctgatc cagccaggcg tagctcagga agtgcatcac
    11701 gacgtagaag aacgtgaaca gaccaagcat gcgccggaag cgcgccagcg cgggcgcctc
    11761 gaaccactgc cgcagcggcg tgatggcgag cgtggcgcac aggaaacgca gggtccagtc
    11821 gccggtcgat cgaatcagcg cctcggccgg gttggcgccc agcgtgtcgg cccacgcgcc
    11881 atacagcagc caggcaaacg gcagcaggca cagcgcgaac agagttggct tcgccgcagg
    11941 atgcagcaga gccgacttgc gcttgcctgc gcccggacgc agcgtcgccg cggggtcgtc
    12001 ggccgtcgta cggatggggt gatcgggtcg gacgctcgac atcgcgctca tggtggcagc
    12061 acttccagcg ggatcagaag aatttcttca gatccatgcc ggcgtacatc tgcccgacct
    12121 ggggctcgta gccattgaac atcagcgtgg cccgcttctt ctggaagagt ccgtcctcgc
    12181 ctatgcggcg ttcgctggcc tgactccagc gcgggtggtc cacggcggga ttcacgttgg
    12241 aatagaaacc gtactcgctg ctggccgcct tgttccaact ggtgcgtggc tccttctcca
    12301 cgaagcgaat ggcgacgatc gacttgccac tcttgaaacc gtacttccag ggcaccaccg
    12361 cgcgcacggg cgcgccgctt tgattcggca gcacctcgcc atagagtccg aaggtcagca
    12421 gcgtcagcgg gttcatggcc tcgtcgaggc gcagaccttc gacgtagggc cactgcagca
    12481 cgcgagaacc gaccccgggc atcatcttct ggtcggccag ggtcacgaac tcgacgtact
    12541 ttgcgttgcc tgtgggctca acgcgcttga tcaattcgga cagtgagtag ccgatccacg
    12601 ggatcaccat cgaccagccc tcgacgcagc gcatgcggta gatccgctcc tccatcgcgc
    12661 cgagcttgag cagttcttcg atgtcgtaga tcctgggctt cctgatctcg ccctcgacgc
    12721 tgactgtcca gggtcgcgtc ttcagcgctc cggcatggcg ggcggggtct gacttgtcgg
    12781 tgccgaactc gtagaaattg ttgtacgagg tcacgtcggg atacggcgtg agcttttcca
    12841 gtgtcatgcc gccgggcgtg gttgtgcgcg ccgacggcag cgctgccagc ttgcccggcc
    12901 gtgcgctggt ttgcgccagc gcgtctcggc cggcccaggc agccaaagcc aggccaccgc
    12961 tgcctgcggc cagggctttc agcaaggttc tgcgctggtc gtagacgtcg cgcggggtga
    13021 tttcgcttgc aacgcggtgg acgaatccct ggtcttcgtg gaaatgcatg tgtctactcc
    13081 ggtgcggcgt tcgagcgtct gttggcgccc aggtgcaagt gtgatggact cgccgggcct
    13141 cgctggctca gcgatgccat gaccttgtgt cgtgccggtc gcttgatcct tacgccggca
    13201 aaggatgcga agacttgagc ggcacccagg tctcgccgct cacatcagat cacttcgtcc
    13261 gccccggaag ccgagaacag gtgcagcgag ttgcgggtgg agcgctcgcg gaaactcttg
    13321 cgggcgaaca acttggcttg cgcctcggct ggcaggtcgg tgatgttgag gacgttcttg
    13381 acgatcagtg tgtcgatcac gtcctcgatc acgcgcacga aatccgcgtc gagtctcgaa
    13441 aagccatcac cctcgggggg gcgtccgacg aacgattgca cctcggcgtg ctcgtcggga
    13501 aggaattcgg tggccgtgtc gtccggctgc ctgtgcaggc tgtcgatggc tcccgaggga
    13561 ttgcggcgga catagggcat gggcgtttct ccggacccgg tccactctac gccgtgcctg
    13621 gcacggtgtg gcgccgaagt gcgtgccaat tgcacgccag taccttcgcc tcggcgcccc
    13681 cgggtcgggc caaagaaaaa gccggtcgtg gaccggctcg gggggctcca gacgccgacg
    13741 ctcagtgcag gccggcgatg tagtccgaga tcgccttgat ctcctggtcg ttcatcttcg
    13801 cggccacgcc agtcatctgt gcgctgttct tgcgcacccc gcctcggaag gcgatcaatt
    13861 gggcttcggt gtactcggcg tgctgtccag ccagccgcgg gtactgcgat ggaatgcccg
    13921 cgccgttcgg gctgtggcag cccgcgcagg cggggatgct gcgctcggca atgcctccgc
    13981 gatagatctt ctcgccgagc gcagccagtt ccgcgttctg ggcgacgcca ggctcggcct
    14041 tcttcgacgc gtagaacgcg gcgacattct tcatgtcgct gtcgctcaaa ggagcggaca
    14101 tgccgctcat gatcgcgttg gcgcgtacgc ctttcttgaa ctccgtcaat tgcttgacca
    14161 ggtactcggg atgctggccc tgcaggatgg gctgggcagg gctgccgcgc gaaccgtcgc
    14221 tgctgtgaca ggcagagcaa accgcagcgg tggcggcgcc cttcgccaga tcaggcttgg
    14281 cgggtgcagg ggattcattc gccgaagcgg tgaacgcagc aagcagaaag caggacaaca
    14341 gagcggacgc aaacttcatg acggatgccg ggtgaagaag aattaaccgg cgattctaca
    14401 attgatgaat ttggcaggcg gcatgatcga gcaaaccaaa gttctcccgt cggctggcaa
    14461 tgcgatcacc gaagcgctgt cagccgaaaa gcgagcgctg gcctggacgc ataccgcgcg
    14521 ctttctcacc acggccagcc agttgcgtga actgccacag accgaattgc ccgagatcgc
    14581 cttcgtcggc cgatccaatg caggcaagtc gacggccatc aacacgatga cgcagcagaa
    14641 gcgtctggcc tttgcatcca agacgccggg gcgcacgcag cacatcaacc tgttcgaact
    14701 ggggccgaag acccgcgccg atgcactgtt cgccgacctg ccggggtacg gctatgcagc
    14761 cgtggctcgc ggtgccaagt tgcgctggca gcaggtgatg gccgattacc tggaagtccg
    14821 acgcagcctg gccgggctgg tgctgatgat cgattcgcgc ctcggcttca ccgacctcga
    14881 tcgccagttg ctgggcttcg tcgggcctcg gatcggcaat gggtccgtca agttgctggt
    14941 gctgctgacg aaggccgaca agctcaatcg cagcgaggcc gctgcagcgt tggtcgccgc
    15001 gcaggaggcg ctcggcgaga tgacgacccc cgaggctgac gtgaacgtgg cgcttttctc
    15061 ggcgctgaag aagagcggtg tcggtgacgc tgccacgctg attcatggct gggtccaggc
    15121 agggcagcgc ccaggcgaac cctcaggggc gaccgaggaa caggtacagc cctaggtcgc
    15181 ctcgcggcgc ataggccact cccaagtaaa gggggccgac acccgtgtcg gctccgacga
    15241 acccgctgaa gcccgcacga agccgcgaca gagatatgtc tttggcctcg atccaggcgt
    15301 tgccggcttc catcgatccg ccgatgaaca tcccgcgcgt gaacaaggga atgtcttcga
    15361 ggcgctggta catggtcagg cggccgaaca ggacgtggct gccggccagc tggttgtagc
    15421 ggtagcccga cagccggtga aacccgccca ggttgtagtt gtagcgaccc agtcctgaca
    15481 ggtcctcgcc tcccgcgctc ttcgcactca tgtgggcatt gaaggtggtg cgcccccagg
    15541 tggtgacgcc ggtgccctcg aactccaacc gagtgaattg ttcgtctccc tgcgggccgg
    15601 aaaccgatcg cgcgccgacc acggcctcgc cgacgacacg gtagccgtgc aacggaaaat
    15661 tgacgaagtc cagctggtcg acggtggcgc tgactcgcag gcccgtctcg tgcacggtca
    15721 acgcgttgcg tgggccgttg aagtccgacg agaccagatc aggctggttg cggaagacga
    15781 cgtggctgac gcccagcctt acctcgccga actcgcccca gggttggccg aggtcgatcc
    15841 ccagcctggc ggatgtgcgc gtgaagcggc cctgctcgtc tccgctgtcg ggttcgtagt
    15901 aggtgatctt gcgccgctcg atcgatgcgt agccggcgac gaaccagtcg tgctgcaggc
    15961 cgatagacca gttgagcggg tggtacagct cggtcagcaa cttcggcgtc tcgccgatct
    16021 gagcgaaatt ccgccattcg gtcccggctt cgtccagcca gcggcggttg tggttcaact
    16081 tgacgttgaa cgcgctgttg ccgcggaagt ccgtgaacag gtcgatgccg acgcgaaagt
    16141 agtgcgggcc ccagggcttg tcttccaggt cgaacaccag cccgtcgcca tcgggcgtcc
    16201 gtatcaggcg gtaatccgcc cgcgtgtagt cgccgctggc ggccagtcga cgggcatcgc
    16261 gctccgcctt ggcgtcgtcg aaaaccgctc ctcccgcgga ttcgagctgg tcgagcagcc
    16321 gctgcggatt ggtcagtttc gatccttcga tgcggatgaa cgccagctgg gcagattgcg
    16381 cttcgccctg gcgatgcgca gcctgccacc gcgcgtagtc cggtggtgac agcgacagct
    16441 ctgccaggcg ctgcaactgg ggctgaccac cggcttcgcc cctgtcgatc agctcgcgcg
    16501 tcttgccgaa gtcgcccgac gtgagcacgc cgagcgtcgg ggcgatcagc acatcccggg
    16561 gctgcagcgt ggcgagactg cgctgcacgt tctgctcggt caggatgctg atcatctgcg
    16621 tggtcaggcc gccaacggac ccgagcgcct cccgactcgc cagcggcgtg ccgatgttga
    16681 ccgcgatcac gatatcggcg cccatggcac gtgccacatc gatgggcagg ttgttcacca
    16741 ggccaccatc gccgaggatc cggccgtccg cttcggtggg cgcaaacacg ccgggcacgc
    16801 tcatgctcga tcgcagcgcc agcgcgagat cgccttcgcg caggatcacg gcctcgcccg
    16861 tctccagatt cgtggccacc gcgcgaaatg gaatggccag gcgggagaag tcgcgcacgc
    16921 tgcgtaccgg cagggtgtag cgccgcagca gagtttccag tccgcggccg gagagggcgc
    16981 cgagcggcgc gcgcaactcg ccgtcacgca tgccgaactc gagcagtggc gacagctcga
    17041 agtcggcctc cttgcgccgc tgggtcagca gcggtcggtc gacccggctc gcaaagactt
    17101 cgtcccaatc gacggcaaga agttcggatt cgaggcggtt ggcatccatg ccgctggcat
    17161 aaagcccgcc gatgattgcg cccatcgatg tgcccgcgat cacatcgatc gggatgcgct
    17221 cgcgctccag caacttgagc acgcccacat gcgcaagacc gcgtgcccca ccccctgaca
    17281 gcaccagtcc gattcgcgga cgagccagtg cccgttctgc gggtgacacg ggttcggctg
    17341 ccgatgccgc gaccgcgaaa cccaggccga tgaatgcaca ggcgctgagg acgacggacg
    17401 gcaagtgcat gccgaaattg tgcgtgaact ccctgccgag gccgagattc gtcagccgat
    17461 ttcgccgagc tggttcctga cccgttcgat cgctcgctgg atgagttcgc tgtccgacag
    17521 cggcttgacc aggccctcgg cagtcagaag ttccagcgcc tgtcgagtga tcgagtgcgt
    17581 gcgtccggct gtctcgccag cgaagacgaa aaatctctgc gagtcgctgc gccacagcaa
    17641 ctgcacgcga cgccagcggc cgctgaggaa ccagcgaaag gcggagccgg gcaccatcgc
    17701 atcgaccgga ttcttcgtcc ccgacggcgg tgtgtcgcca tcggacatca ggtcggccgg
    17761 gatggtgtcc atggagaaca cgtcgatcag cgaatccgcg aatcccttgc cggcctgcac
    17821 caggggctcc gcaggctcct cttcgcgcag gcgcttcatg atttcctgcg gagtgggttc
    17881 cctggtgtct gcgcgagcgc cgggacgcag ggcttcggtg tgtatcgcca tcaactcgtc
    17941 gagcacggct tgttggtctg catcgggcag ggcaatcagc gccatgccgg accgcagtcg
    18001 ctgcagcagc gtcggaagca gtgccagcag acgctgccga ctttgcgggt ggtccggcag
    18061 gcgcaggctc cacagcaggt cgtccacggc cttcaggtag ccagcggtgc gctcgtcggc
    18121 gtcgccgtac cgcagcatcg actcggtcac cacgcaagcc caggactgaa ccaggaaatt
    18181 gcggatgcgc ggcgtggtgt gcaccgagcg aatctgttca gccagctggt gtgacagctc
    18241 gcgctccaga acctcccggc gttcggtcgc cttcaatgcc tcgatcgtcg actgggcttg
    18301 ctcctgctgc tggcgcagtt cgcccgcaag atgcgattcg agtcgctgca gtccttcggc
    18361 gtaaagagat gccgtctggt tcgcggcgcc tgcgatctcg tgggccagcg attcgcacag
    18421 cgtcgtcagg cgatgggctc gcacgtcgcc cggctgtggc cagacttgtg aagccgaggc
    18481 gatgcggttc atcaatgccc acacggggtg ctgatggccc gcgagcatgc tgcgatccac
    18541 cagtgccacg cgcagggcag aaacctgcag gcgtgccatc acggcacgca ccgcggccga
    18601 gagctgggtg tccgacagca cggactcgaa gattcgcgag aggagctcga cgatctgtcg
    18661 atcgaccact tcctgagcat gggcgagcag ggccgcccgg tgctcgcgca gaggggcggc
    18721 gtccagcatg gggggcggtt cggccccggc cccgccagat tcggcgcgtc tctggagcag
    18781 gagttctatc tgctggagcg cctgttcgaa ggccgcagtc agcctggagc tgtcgtgggt
    18841 cttcgcagca gctgacggtg aatccgcgct cggccccagg gttcgcaacg tgactgcgcc
    18901 agtgtcgctg ccggcgggag cgccgccgcc ctgcgatcct ggcatcctgt tcagcaactg
    18961 ttcgaagcct tgctgttctg aagagttgtt cctcgaggtc gcagcagtct gcagcccgct
    19021 cggcagaacc atcgtccggt agatgctggg ttccacccct tgcgattcca gtcgcgtgca
    19081 ggcgccagcc caggccatct tgagctctgc ggccatgctg gacgctgccg tgcgcagcag
    19141 caggctgcgt tgtgccggag tgtcgttgat cgcgcagacg gcttcccaaa gtgcctgtgc
    19201 gtaggccgcg ggacgcagcg ggttcgagtc ggcgctgacg tgagtctgtc cggtcagcgc
    19261 cgaagtgaag gtctgcaatt cacgcaattc ccattcggcg atgctgtcga tcagctggga
    19321 tgcccgagag atctcgatgt ctgcttcgac ccgggtttcg tccatcaact gcaggccgtc
    19381 gattgcggtc acgtgttcgg ttgatgtgcc gctgtcgaga ccctggattt cggccgtcac
    19441 ggtgcggctc aggccgtcga tgaatgtctg ggcaaaccgt cgctggccct gctggagtga
    19501 atcgaccgac tcgcgctgaa catgacgctc ggatgcagtc gctggcgcac ccggcttggc
    19561 cgctctctgc aaatgcgcca gggtcgtcgt caccgtgcgc tcgatcagcg cggacgaccg
    19621 tgacaactcg tcgtctacga aacgcagcat cagctgagaa accggcatgg aggggccttt
    19681 gctgggtcga tcgtgaccct ctggaattat ctggccgatc gtgcggcgcc aaggtgtcgg
    19741 cgttgcgctg cgagtccaaa gaaaagggct gccgaagcag cccttttccg tgcagcgatc
    19801 cgggcgcgtg agcccgaacc tggacaattt acatgtccat gccgcccatg ccacccatgc
    19861 cacccatgcc gccaggcatg ccgccgccgg cggattcttc cttcggcgct tcagcgacca
    19921 tggcttcggt cgtcagcatc agcgacgcga ccgatgctgc gttctgcagc gcggtgcgtg
    19981 tcaccttcgt cggatcgaga atgcccattt cgatcatgtc gccgtaggtg tcattcgcgg
    20041 cgttgaagcc gtaattgccc ttgccggcca acaccgcgtt caccaccacg ctggcttcgc
    20101 cgccggcgtt gtagacgatc tcgcgcagcg gagactcgat ggccttcagc accagcttga
    20161 tgccggcgtc ctgatcgggg ttgtcaccct tgatctcgcc tgccagttgc ttggcgcgca
    20221 gcagggccac gccgccaccg gccacgatgc cttcctcgac cgcagcacgc gtggcgtgca
    20281 gggcgtcttc gacacgcgcc ttcttttcct tcatctcgac ttcggtggcc gcgccgacct
    20341 tgatcacggc aa
//
LOCUS       DAOORV010000061        20214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3248_length_20214_cov_9.021578, whole genome shotgun sequence.
ACCESSION   DAOORV010000061 DAOORV010000000
VERSION     DAOORV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_20214_cov_9.021578"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKV56_09775"
     CDS             complement(<1..374)
                     /locus_tag="PKV56_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68141.1"
                     /translation="MPYQIHWEDRGVVFKYLGHASDADIAAATRCVQADARYDSLLYA
                     LHDFLASESFTFSQAAMEELAAVNAAAARSLRAGKLAIAVMTEMEEVVAAIHAYVASG
                     LSAHDFRIFKSVDPARAWLKGMT"
     gene            complement(554..2467)
                     /locus_tag="PKV56_09780"
     CDS             complement(554..2467)
                     /locus_tag="PKV56_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOX68142.1"
                     /translation="MTTEAALNEATRSPDLARRQGTALLTLGAIGVVYGDIGTSPLYT
                     VKEVFAPATGVPLDAHHLIGAVSVIFWALMLVVTLKYVILILRADNHGEGGGLALTAL
                     AAQAVRTRPALRTALLLLGVFGATLFYGDSVITPAISVLGAVEGLQVLAPSLQPFVVP
                     ITVVIVTGLFLVQRLGTAVVGRYFGPIIVLWFVTLGATGVMHIAEQPAILAALNPLHA
                     WEFLSERGWKLFAAVGAIVLALTGAEALYADMGHFGRRPIQLAWAGLVLPALALNYMG
                     QGALLIGDPSAIENPFFRMFPEAWLLPAVMLATLAAIIASQAVISGAYSMTKQAVQLG
                     FLPRMSVLYTSARESGQIYMPAVNWALLAGVLAAVLYFGSSSALAGAYGIAVTLTMMI
                     TTVLTYFVIRKGWHLPGSVAVGATAFFLAIDALLVAGCAIKFLDGGWFPLALALLLFG
                     TMSTWSRGRELLMNTIRRNGLALQPFIDSLDPKGIHRAERTAVYPVADPSTVPQALLH
                     NLKHNQVLHERNVILTVVFREVPWVAMSERLEVASLGHGFWRVTLHFGFMQTPDVPRA
                     LTLAESHGLRIPAFETSFFLSRETVVPTPGGGMANWRERLFSAMSRNAGGVVEFFRLP
                     GNAVVELGSRVEI"
     gene            complement(2545..3252)
                     /locus_tag="PKV56_09785"
     CDS             complement(2545..3252)
                     /locus_tag="PKV56_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX68143.1"
                     /translation="MSLCVLLVEDDRELRSTLREALVVEGYRVLAAASLADADCLLTH
                     AETQGGIDLVLLDLGLPDGDGEALLSALRRGRCTAPVIVISARHCDDPKIRLLDAGAD
                     DYLVKPFGLGELLARMRVALRHRGTALQPAITRYEHGGLRIDLVAHRVSLAGAEVHLT
                     PTEFKLLARLVRQAGQVVTHRQLLADVWGAEFTEHTHYLRLYMAQLRAKLEAEPADPQ
                     RLLTEPGVGYRLAEPAA"
     gene            complement(3249..4808)
                     /locus_tag="PKV56_09790"
     CDS             complement(3249..4808)
                     /locus_tag="PKV56_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68144.1"
                     /translation="MNSAPARWLGAVVWALSWPLMLLLDGHVDLASLAQVLILAAALA
                     GVWLSPIPSMLACAAAVLAFNFFFVPPRGTFTVDVHQHALLLITMLAVSWIVSLLMSR
                     QRQLACAERLHALRAEQLQGLGDTLRDAHDPRDRGAQLQGMLSELVGAPAALLMVVQP
                     SRIANEQPSFIQLGEVDDDERAGLGLTQLQGRAMGPGTGRHEEQAAWYLPLRGRQSSF
                     GAALLRLPALPTDASTVRGHAQALCDQMGLAIERALAAETAAAAREEAQAQNLRNTLL
                     MAISHDYRTPLATIMSSASSLHDQADRLSIEQRRRLAATIVDEADQLARLTANTLQLA
                     RLDMPGVVLRRDWESVEEIVGAVVRRVRQRAPAQRVKLRLEPGLPLLYCDAVLLVQML
                     ENLVDNALRYGGEGAPVEVLARRAGDQVLLAVRDRGPGVAPAWRDRIFDVFQRGDAAQ
                     ALPASADTSAHRGAGVGLAMCRAVARAHGGELRLRARSHGGSSFECALPVVDPPAPEP
                     ETAGQERRLPG"
     gene            complement(4888..6129)
                     /locus_tag="PKV56_09795"
     CDS             complement(4888..6129)
                     /locus_tag="PKV56_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOX68145.1"
                     /translation="MNTSARQDSAFQISPAAQRSLRVSVVTETYPPEVNGVSLTLARV
                     VEGLHRRHHDVQLIRPRQGPSDSPRNAPRFREVLMRGLPIPRYPNLRMGLPSRRALVK
                     LWAADRPDVVHIATEGPLGWSALRAALQLKLPISSDFRTNFHAYSHHYGIGWLNRPIM
                     SYLRGFHNRTGSTMVPTDALRRELEQAGLRQLTVVSRGVDTVQFAPGQRSDDLRRRWG
                     AEPGDLVVAFVGRLAPEKNLGVVLAAFEAIRGVHARARLVLVGDGPQRAELQSLCPSA
                     IFAGHCHGQDLAAHYASADLFLFPSLTETFGNVTTEAMASGLPMVAFDHAAAAQLIRS
                     GDNGMLANCADVPAFVKAAVDLAADPERRRLIGERARHTACALDWDVIVARFEAVLSS
                     LLHQVPQSEPPLPVLLGQAPS"
     gene            6264..6872
                     /locus_tag="PKV56_09800"
     CDS             6264..6872
                     /locus_tag="PKV56_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68146.1"
                     /translation="MQSFLQALEEQRWDDHRYYHHSRINQSLHLVSAISFVVAYGLLF
                     VDPAMAAILAWGVSMTTRQAGHFFFEPRGYDEVNQVTDAYKEEVKVGYNIQRKIVLMG
                     AWAAVPVLLWLSPSVFGLIAPAESFNGYVHDVGIGWLALGVAGLFVRVLQLWMKADLM
                     TGLVWMTKILTDPFHDIKLYHRAPLHLLRGELVDTKPHERAE"
     gene            complement(6929..8581)
                     /locus_tag="PKV56_09805"
     CDS             complement(6929..8581)
                     /locus_tag="PKV56_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX68147.1"
                     /translation="MDESFALSALTLGILAWAFPRAKRRMELSKAKHRSLAGHSRMAK
                     RIASLIPGYAYDEDGFFGSDGAAAEAVARRRAGFQRLSRLYDERYPRSAAMTARARDS
                     VSDLQFTSSYRVPFQYSPYLRKHLRSGTFLKESHGVTVEDLDGNVFYDLTGSYGVNLF
                     GVDFYKECIDEGVARVRDLGPVLGAYHPCVIDNVERLKAISGLDEVSFHMSGTEAVMQ
                     AVRLARYHTGRKNLVRFCGAYHGWWEDVQPGPGNPLPPRETYTLRDMHEGSLKVLRTR
                     RDIACVLVNPLQALHPNSGAPADSSLVDSGRRAGFDREAYTAWLRALREVCTERGIVL
                     IFDEVFVGFRLAPGGAQQYFGVAADMVVYGKTLGGGLPVGVVCGRAALMKRFREDRPA
                     DVCFARGTFNAHPCVMGAMGAFLDRLDGPQVKSMYDGIDARWDARAQQFNEALQSGGL
                     PVRIAHLSSIWTVTYTQPSRYNWMLQFYLRAHGLALSWVGSGRIIFSLNYSDEDFAAV
                     LQRFVAAAHEMQTDGWWDGAATSNRLIRRGILREMLRQRLRE"
     gene            8645..9511
                     /gene="asd"
                     /locus_tag="PKV56_09810"
     CDS             8645..9511
                     /gene="asd"
                     /locus_tag="PKV56_09810"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517196.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOX68148.1"
                     /translation="MNPLRSLVANEDLNFLLTNRVPRMLLTRWMGWFSQIRNPWIARA
                     SIAVWRLFTDLDLSDARKQRFASLHDCFTRELRDGARTIDRRADILSSPCDAIVGACG
                     RVDDGQVFQAKGFPYAIRDLFGPSQDTSAFRRGTYVTLRLTSAMYHRFHAPQDCEVEH
                     LTYLSGDTWNVNPIALARVEQLFCRNERAVLRLRLKDSGHTIALVPVAAILVASIRLH
                     FLDVLLHMRYRGSAEIACSARFTKGQEMGWFQHGSTILVFAPPGFSLCDGITPGQHIR
                     MGQALMQLGRRG"
     gene            complement(9581..10441)
                     /locus_tag="PKV56_09815"
     CDS             complement(9581..10441)
                     /locus_tag="PKV56_09815"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOX68149.1"
                     /translation="MDNTEEDGSLNVRTAWISDLHLGTPGCQALALLEFLRTTDCQTL
                     YLVGDIIDGWQLRRSWYWPQSHNDVVQKILRKARKGTRVIFVPGNHDEFARRYVAHNF
                     GGVDIVEDCVHVTADGRQLWVTHGDLFDGVIQCAKWLAYVGDIAYEFTLRLNRRFNVA
                     RARLGLPYWSLSRFLKLKVKRAVSYVGDFEAALARETRSRGLDGVVCGHIHHAEIREI
                     EGVLYCNDGDWVESLTALVEHHDGRLEIIDRSAEARQVAVAPVRTESALLPMPGAVSP
                     VHCADIAPRT"
     gene            10632..11498
                     /locus_tag="PKV56_09820"
     CDS             10632..11498
                     /locus_tag="PKV56_09820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOX68150.1"
                     /translation="MREHPLNTLPMFDTHLNMNPSRHAARLHVPHAPTVYRQKAAAAP
                     VSFEAVWARDLDEVRLAQQLRHLVFVEEMGARVSLPAGTPPGHDADLFDDHCEHLLLR
                     VTTTEGEPGPVIGTYRVLTPAAAKRVGGLYSETEFDLTRLRSMRDRMAELGRSCVHPD
                     WRTGGAILALWAALAEFMKRNALDTMMGCASVGMRDGGHVAASLWTQLRGTHLAPIDW
                     QVRPRLPLPVEQLQCDLDVDPPALIKGYLRCGAKVLGPPAWDPAFNTADLPMLIRLAD
                     LPPRYRKHFLGE"
     gene            complement(11583..13628)
                     /locus_tag="PKV56_09825"
     CDS             complement(11583..13628)
                     /locus_tag="PKV56_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX68151.1"
                     /translation="MSAVFPSMSAPPTQVTRTPDNAGGFFAYHGLWAPGVRLFRRLRF
                     LSKAMIISLAFTLPLLGALAWDQMSSYEHALQTRMDATRQQVEVAHGLIVWAQAQEAA
                     GNLTRDQAQKTVLSALEAVRYDSKEYFWVNDMQPKILMHPIKPELNGKDVGDMKDPNG
                     LALFKAFVAEVRQHGKGFVAYQWPKPGKSKPQDKVSYVIGFEPWGWVVGSGVYIEDLR
                     SDALEKAAWTSGVVAASLIIAAYLFLCFYRVMDGGLRETRRHLRAMTSGDLTTSPSPW
                     GGDEAAQLMLELRAMQESLRGMVLRVRRSSDEIVHSSSEIAAGAADLSARTEQAAANL
                     EESAASMEEISSTVKSTAEHTREASEMARHNAKTAADGGRVMRDVVHTMEGIRNSSNQ
                     IGEIIGTIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAKRSADAAREIK
                     ALIDRSVEQVETGTSIVRSAGTTIDEIVASSQRVDQLLGEVATGAREQSLGIGQIGQA
                     VQDLDRMTQQNAALVEQTAAAASAMKDQAHTLADEVARFKTPVGMALTVAAESGAHAD
                     FDFDKAIEAHRQWKVKLRKAIADRENLDADTICRDDQCPLGKWIHGSGGVQWGTRPNF
                     VALLDKHAEFHQTAAGVARQINAGQYAQAEQLIGAGSRFAQVSTEVATILTHAKRGM"
     gene            complement(13980..15221)
                     /locus_tag="PKV56_09830"
     CDS             complement(13980..15221)
                     /locus_tag="PKV56_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOX68152.1"
                     /translation="MAALMHCRRCLYTSAHPLGLMLDAEGICSGCRVHEEKDRLDWAE
                     RWQRLESLLAPYRSRSGETYDCIVPVSGAGDSHYIVHLVKQRLGLNPLLVTYNKYFNT
                     SLGIRNLANLRIRFDCDLIQQNVNPHAVKKITRATLRDFGSDYWHCLAGQTVFPVQTA
                     VRYRIPLILWGAHQGLEQVGMYSHEDEVEMTRRHRQDHDLMGREADDLPTMFDTLKES
                     DIWQYRYPDDAALSAIGVRGVYLGNYVRWDPKAQHEQMIERCGYRTAAFARTFDTYDH
                     VDCWNYMDLHDVLKLHKHGYSKVTDHACREIRHGRLTRAQGLALVRRHEQAAPQHLDK
                     FCEWLGLDRSGLGFLLDRHRNPRWWREAETGRWAFAGASTTAGEAAGVTMPPASAVFE
                     ANDVLAHDGIDGYITIGKGHP"
     gene            complement(15226..16011)
                     /locus_tag="PKV56_09835"
     CDS             complement(15226..16011)
                     /locus_tag="PKV56_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOX68153.1"
                     /translation="MLEKRLIGVITVKDGWAVQSIGYRRHRPLGRPEVIAENLDRWGV
                     DEILLQCIDRDAQGGGPDLALLERLCRRGLSTPLVYTGGVRSVSDGVRAVQAGAERIG
                     IDALLHDDPHLAAELAEPLGAQALIAVLPLSVHNGALAWLDHRSRLSTPLAPALLDVL
                     ASGAISEVLLVDWQHEGVHEGFDERLLALWPMPTLPLIAFGGLSEPSQLRGVLERPNV
                     VAAAVGNFLNHREHAVQRLKQSLAGLPLRAATHAPLDPARAWS"
     gene            complement(16004..16621)
                     /gene="hisH"
                     /locus_tag="PKV56_09840"
     CDS             complement(16004..16621)
                     /gene="hisH"
                     /locus_tag="PKV56_09840"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOX68154.1"
                     /translation="MSGARLTIGVVDYGAGNLASVARAITGLGHRCRVTRDTETLAAA
                     DAIVLPGVGAFPAAMASLHEHGLVDFLRQQARSGQPLIGICLGMQLLADMSLEHRLTA
                     GLGLIPGRVRPLAGARWHIGWNTIEIDPEDALLSPSDGQSLYFNHAYAFDTPPPYCIG
                     VSRLADAFTVAVRRGKVVGLQFHPEKSQLAGRLLLKNVIEGLCRA"
     gene            complement(16618..17745)
                     /locus_tag="PKV56_09845"
     CDS             complement(16618..17745)
                     /locus_tag="PKV56_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOX68155.1"
                     /translation="MRYCLRCLQPDTRPNTRFSDAGICPACDYVDAVRGVDWQERIDI
                     LTDLFASRRRKPGQFHDCIIGVSGGKDSTRQALWVRDKFDVNPLLVCLGYPPEQATDR
                     GMHNLSNLIDLGFDVLVSQPAPATWRTLKRQGFERFTNSFRSTELALFSAVPQIALRY
                     GIDLILWGENPALQLGDLKTLGKTGYDGNNLRHMNTLSSGLQWMLDAGHERRQLLPYV
                     YPQPEEFDALGLQIIYLGWFLGDWSLVNNAMHSCAQGLEIRTDGVADTGDLFGVTALD
                     EDFTPINQMIKYFKFGFGRTTDYVNEEIRLGRMSRERGIELVEQHDHACSPDYIARYC
                     DYLGIGVDAFWRQVHAATNRTLFDIDGDGRIHRRFQVGVGL"
     gene            complement(17771..18610)
                     /locus_tag="PKV56_09850"
     CDS             complement(17771..18610)
                     /locus_tag="PKV56_09850"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOX68156.1"
                     /translation="MQTALASRPAARDPVVVISRTSSRDPALQLARQGWYAARGVFGP
                     DAVQALRHEVEDVFSSPKHAGSILTPPGCGGVLLDLFRFPGIYRRLFQPHIAEVLTEL
                     IGPGFVLLPEHAAHRDGFGGWHKDTDMFEYAGSMAHWSADHAIYQCAVYLQDNSAEHG
                     GGVSVVNGSHLVPKPAAGQPGAAQRFQDRAQQQGTVLGSRGGDLVVFHTRLDHRATPT
                     SGAAPYGAKLALFFIAARDNHHAAAYSEFIHRRKDYTYLNTYRVPPEMQALAREQGFR
                     FAD"
     gene            complement(18623..19639)
                     /locus_tag="PKV56_09855"
     CDS             complement(18623..19639)
                     /locus_tag="PKV56_09855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX68157.1"
                     /translation="MTTTPETLSIAEIRGPQFMARQREAMARDHARLHEHLGDFVAVP
                     CPSCGQTAASRAFDKYRCQFVRCHDCDTLYMSPRPTPALMDDYYSHSENYRLWAEHIF
                     PASEERRRDKLCRPLLATIAAACAAHGVRPGHLIELGPGFGTFAALARDSGAFERVSV
                     VERTPEMAAACRRHGLVVHETAVEDLPERFDDPADVLVCFEVIEHVYDPVPFLARALR
                     LLKTGGLLVLTCPNGNGFDTQVLGAESVAVDTEHVNLFNPASIARLLERLDCEVLEVA
                     TPGRLDVELVREAVQAGRCDVSGQPFLQQLLVTDHARLAAPFQQFLADHGLSGNLRAI
                     ARKR"
     gene            complement(19655..>20214)
                     /locus_tag="PKV56_09860"
     CDS             complement(19655..>20214)
                     /locus_tag="PKV56_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOX68158.1"
                     /translation="RAAGCRDLVLLKCTSTYPSTPLNTNLRSIPVLRDAFGCEVGLSD
                     HTMGCGAAVAAVAFGAVVIEKHFTESRAEGGVDSAFSLEPAEFAQLRIESERAWQALG
                     APSFGMSVAETASMRYRRSLYIVEDLKAGDVLTAANLRAIRPGYGLPPRELDALLGCS
                     VCQDVRRGTPASWSLVHPASAVLAT"
BASE COUNT         3425 a         6835 c         6749 g         3205 t
ORIGIN      
        1 gtcatgcctt tcagccaggc acgtgcggga tcgaccgact tgaagatgcg gaaatcgtgg
       61 gcgctgagtc cggaagcgac gtaggcgtga atcgcggcca cgacctcctc catttcggtc
      121 atgacggcaa tcgccagctt gccggcgcgc agcgaccggg ccgcagctgc gttgaccgcg
      181 gccagttcct ccatggccgc ttgcgagaaa gtgaaactct cgcttgcgag gaagtcatga
      241 agcgcgtaca gcaggctgtc gtagcgcgca tccgcctgca cgcagcgcgt ggccgcggca
      301 atgtccgcgt cgctggcgtg ccccaggtac ttgaagacga cccctcggtc ttcccaatga
      361 atttgatacg gcatcgatgc agcgagttct cacgcatcga cgtgacgaac ccataaaaaa
      421 ccagcaaatg gacaattgat ccaaatcaac caaaacgaat atacaggatc gatgcaccgg
      481 ggcccgagcg gcccgccgcc atggccagct ggacgacggc agaccgggcc gcttgacgcc
      541 ttgcgcgtga agttcagatc tcgacacggc tgcccagctc caccactgca ttcccgggca
      601 gcctgaagaa ttcgaccacg ccgccggcgt tgcggctcat ggccgagaac aggcgctcgc
      661 gccagttggc catgccgcct ccgggagtcg gcaccaccgt ctcacggctg aggaagaagc
      721 tggtctcgaa cgccgggatg cgcagtccgt gtgactcggc caatgtcagg gcccggggaa
      781 cgtccggtgt ctgcatgaag ccgaaatgca gcgtcacgcg ccagaaccca tggccaagtg
      841 acgccacctc cagccgttcc gacatcgcca cccagggcac ttcgcggaac acgaccgtca
      901 ggatcacgtt gcgctcgtgc agcacctggt tgtgcttgag gttgtgcagc aacgcctgcg
      961 gcacggtgga gggatcggcg accggataca ccgcagtccg ctcggcgcgg tgaatgcctt
     1021 tggggtccag gctgtcgatg aagggctgca gtgccaaacc gttgcgacgg atggtgttca
     1081 tcagcagttc gcgcccgcgc gaccaggtgc tcatggtccc gaacagcagc aacgcgagag
     1141 ccagcgggaa ccagccgccg tccaggaact tgatggcgca cccggcgacc agcagggcat
     1201 cgatggccag gaagaaggcg gtggcgccga cggccaccga ccccggaagg tgccagccct
     1261 tgcggatcac gaaataggtc agcaccgtgg tgatcatcat cgtcagcgtg acggcgatgc
     1321 cgtaggcacc agccagtgcc gaggaacttc cgaaatacag caccgcggcc agcacgccgg
     1381 cgagcagcgc ccagttgacc gccggcatgt agatctggcc cgattcgcgc gccgaggtgt
     1441 acagcaccga catgcgtggc aggaagccga gctggacggc ctgcttggtc atcgagtaag
     1501 ccccggaaat gacggcctgc gaagcgatga tggcggccag ggtggcgagc atcaccgccg
     1561 gcagcagcca ggcctcggga aacatccgga agaacgggtt ctcgatggcc gatgggtcac
     1621 cgatcagcag cgcaccctgg cccatgtagt tcagcgccag cgcgggcagc acgagcccgg
     1681 cccacgccag ctggatcggt cgtcggccga agtgacccat gtcggcatag agggcctcgg
     1741 cgcccgtcag cgccagcacg atggcaccga cggctgcgaa cagcttccag cctcgctcgc
     1801 tgaggaactc ccaggcgtgc agggggttca gcgcggcgag gatggccggc tgctccgcga
     1861 tatgcatcac tccggtggca cccagcgtga cgaaccacag gacgatgatc ggcccgaagt
     1921 agcggcccac cacggccgtg cccagccgct ggaccaggaa caggccggtc acgatcacca
     1981 ccgtgatcgg cacgacgaaa ggctgcagcg atggcgccag gacctgcagc ccctcgaccg
     2041 cgccgaggac cgagatggcc ggcgtgatca cgctgtcgcc atagaacagc gtcgcaccga
     2101 atacgccgag cagcagcaac gccgtgcgca gagcgggtcg cgtacgcacc gcctgcgccg
     2161 ccagcgcggt gagcgccagg ccgccgcctt cgccgtggtt gtcggcccgc aggatcagga
     2221 tcacgtactt cagcgtgacc acgagcatca gcgcccagaa gatcaccgag accgcaccga
     2281 tcagatggtg cgcgtccagc gggacgcccg tggcaggcgc gaagacctct ttcacggtgt
     2341 acagcgggct ggtgccgata tcaccgtaca cgacgccgat cgcgcccagc gtcagcaggg
     2401 ccgtgccctg acggcgcgcg aggtcgggcg atcgggtcgc ctcgttgagt gcagcttcgg
     2461 tggtcatgtc tttccgggaa gagtggtcat gccgacagcg tgcgcgcgag ggcatcagga
     2521 ccgcataagg gtcccgtgcg tcaatcaggc ggctggctcg gccagcctgt agcccacccc
     2581 gggctcggtg agcagccgct ggggatcggc gggctcggcc tccagcttgg cgcgcagctg
     2641 ggccatgtac aggcgcaggt agtgcgtgtg ttcggtgaac tcggcacccc agacatccgc
     2701 cagcagctga cggtgcgtca ccacctggcc ggcctggcgc acgagccgcg ccagcagctt
     2761 gaactctgtc ggcgtcagat gcacttcagc gcccgccagg ctgacgcgat gcgccaccag
     2821 atcgatgcgc aggccgccgt gctcgtagcg cgtgatggcc ggttgcagcg cggtgccgcg
     2881 gtgacgcagt gcgacgcgca tgcgagccag cagctcgccg aggccgaacg gcttgaccag
     2941 gtagtcgtcg gccccggcgt cgagcagccg aatcttgggg tcatcgcagt gtcgagccga
     3001 gatcacgatg accggcgcgg tgcagcgccc gcgccgcaac gccgagagca gcgcctcgcc
     3061 atcgccgtcg ggcaggccaa ggtcgagcag cacgagatcg atgccgccct gcgtctcggc
     3121 atgggtcagc aggcaatccg catcggccag gctggcggca gccagcaccc tgtagccttc
     3181 gaccacgagg gcctcgcgca acgtgctgcg caactcgcgg tcatcctcga ccagcagcac
     3241 gcacaggctc atccgggaag tctccgctcc tggccggcgg tctcgggctc gggcgcgggc
     3301 ggatcaacca ccggcagagc gcattcgaag ctcgaaccgc catggctgcg cgcgcgcagc
     3361 ctgagttcgc ccccatgggc acgcgcgaca gcacggcaca tcgccagacc gacgcctgcg
     3421 ccacggtggg ccgaggtgtc ggccgaagcg ggaagcgcct gcgccgcatc gccgcgctgg
     3481 aacacatcga agatgcgatc acgccaggcc ggtgccacgc cgggaccgcg gtcgcgaacc
     3541 gcgaggagaa cctggtcacc ggcgcgacgc gccagcacct cgaccggcgc accttcgccg
     3601 ccgtacctga gcgcgttgtc caccaggttc tcaagcatct ggaccagcag cacggcgtcg
     3661 cagtacagca gcggcagacc gggctcgagg cgcagcttca cgcgctgcgc cggagcacgt
     3721 tgccgcacac gccgaaccac ggcgccgacg atctcctcga ccgactccca gtcccgccgc
     3781 agcaccaccc ctggcatgtc cagccttgcc agctgcagcg tgttggcggt caggcgagcg
     3841 agctggtctg cttcgtcgac gatggtggcg gccagacgcc ggcgctgctc gatcgaaagc
     3901 cgatcggcct gatcgtgcag cgacgaagcg gaactcatga tggtggccag tggggtgcgg
     3961 tagtcgtgcg agatggccat cagcagcgtg ttgcgaaggt tctgggcttg cgcctcttcg
     4021 cgtgcggctg ccgcggtctc cgctgccagg gcacgttcga tcgcgagccc catctggtcg
     4081 cacagggcct gcgcatgacc gcgcacggtg gatgcatccg tcggcagggc aggcagccgc
     4141 agcagcgctg caccgaaact cgactgccgg ccgcgcagcg gcaggtacca agccgcctgc
     4201 tcctcgtgac gaccggtgcc cggccccatg gcccggccct gcaactgggt gagacccaat
     4261 ccggcccgct catcgtcgtc cacctcgccg agctggatga atgagggttg ctcgttcgca
     4321 atccgtgacg gctgcaccac catcagcagc gcggccggcg cgccgaccag ctccgacagc
     4381 atgccctgca actgcgcccc gcggtcgcgc gggtcgtggg catcccgcag cgtgtcgccc
     4441 agcccctgca gctgttccgc ccgcagggca tgcaagcgct ctgcgcaggc cagctgccgt
     4501 tgccgcgaca tcagcaggga cacgatccag ctcaccgcca gcatcgtgat cagcagcagg
     4561 gcatgctggt gcacgtcgac cgtgaacgtg ccgcgcggag gcacgaagaa gaaattgaat
     4621 gccagcacgg ctgcagcgca ggccagcatc gacgggatcg gcgacagcca gactcccgcc
     4681 agcgcggccg ccaggatcag gacctgcgcc aggctggcca gatcgacgtg gccgtccagc
     4741 agcagcatga gcggccacga cagcgcccac accaccgccc ccagccatcg cgcgggcgca
     4801 gagttcatcg ggcgagggtc agggccatgt gcacggcgca caagctagca cgcgccgcgt
     4861 caggaacgca tcaggaacgc ctttgagtca ggagggggcc tgaccgagca gcacaggaag
     4921 cggcggctcg gactgcggca cctgatggag cagcgacgac agcacggcct cgaagcgggc
     4981 gacgatcacg tcccagtcga gcgcgcaggc cgtgtggcgg gctctctcgc cgatcagcct
     5041 gcgacgctcg ggatcagccg cgagatcgac cgctgctttc acgaacgcag gcacgtccgc
     5101 gcagttcgcc agcatgccgt tgtcgccgga gcggatcagc tgcgccgccg ccgcatggtc
     5161 gaacgccacc atcggcaagc cactggccat cgcctcggtg gtgacgttgc cgaaggtctc
     5221 ggtcaggctg ggaaagagga acaggtcggc cgacgcgtag tgcgctgcca gatcctgccc
     5281 gtggcaatgc ccggcaaaga tcgcgctggg gcacagcgac tgcagttcgg cgcgctgcgg
     5341 cccatccccg accagcacca gacgcgcccg ggcatggacg ccgcggatcg cctcgaacgc
     5401 cgccaggacc acacccaggt tcttctccgg cgccagtcgc ccgacgaacg cgacgacgag
     5461 atcgccgggc tcggcgcccc agcggcggcg caggtcgtcg ctgcgttgcc caggcgcgaa
     5521 ctgcaccgtg tcgacgccgc gcgacacgac ggtcagttgc cgcagtccgg cctgttcgag
     5581 ctcgcggcgc agcgcatcgg tgggcaccat cgtgctgcct gtgcggttat ggaacccgcg
     5641 caggtaggac atgatgggcc ggttgagcca gccgatgccg tagtggtggc tgtaggcgtg
     5701 gaaattcgtc ctgaagtcgg agctgatggg cagcttcagc tgcagcgccg cgcgaagcgc
     5761 agaccagccc agtggccctt cggtggcgat gtgcaccacg tcaggccgat cggcggccca
     5821 cagcttcacc agggcacgcc gggacggcag ccccatgcgc aggttcggat agcgcggaat
     5881 gggcaggccc cgcatcagca cctcgcgaaa gcggggggca tttcggggcg agtccgaggg
     5941 gccctgtctc ggccggatca gctggacgtc atggtgtcgc cggtgcaggc cttcgaccac
     6001 gcgggccagc gtcagagata ccccgttgac ttcgggcggg taggtttcgg tgaccaccga
     6061 aacacgcagc gatcgctgag cagccgggct gatctggaac gcactgtcct gccttgccga
     6121 tgtattcatg caggcatggt gcacatcgaa cgcaacagtt cgatgacagg tcgctgcgtc
     6181 acgctggttt cacgcaacgc ggtcaacatg tccccagggc gcgccctccg gtgcgtccat
     6241 caacccagac gagggggcac gccatgcaat ctttccttca ggcgctcgaa gaacagcgct
     6301 gggacgacca ccgctactac caccacagcc gcatcaacca gagcctgcat ctggtgagcg
     6361 cgatctcgtt cgtcgtcgcc tacgggctgc ttttcgtcga tccggcgatg gcagcgatcc
     6421 tggcctgggg cgtctcgatg accacccggc aggccggcca cttcttcttc gagccgcgcg
     6481 gctacgacga ggtcaaccag gtgaccgatg cctacaagga agaagtcaag gtcggctaca
     6541 acatccagcg caagatcgtc ctgatgggcg cctgggctgc ggtgcccgtc ctgctgtggc
     6601 tgtcgccctc ggtgttcggc ctgatcgcac ctgccgagtc gttcaacggc tacgtgcacg
     6661 acgtaggcat cggctggctg gcgctgggcg tcgcggggtt gttcgtgcgg gtgcttcagc
     6721 tgtggatgaa ggccgacctg atgaccgggc tggtgtggat gacgaagatc ctgaccgacc
     6781 cgttccacga catcaagctc tatcaccgcg caccgctgca tctgctgcgt ggcgaactgg
     6841 tcgacaccaa gccgcacgag cgggccgagt gaacccgatc ccggccagcc ggtcggccgc
     6901 agtgcgcggc cgaccggccc gggccatctc actcgcgcaa tcgctgccgc agcatttccc
     6961 gcaggatccc tcgccggatc aaccggttgc tcgtcgcagc gccatcccac catccgtcgg
     7021 tctgcatctc gtgagccgcc gccacgaagc gctgcagcac ggcggcgaag tcctcgtcgc
     7081 tgtagttgag gctgaagatg atgcgtcctg aaccgaccca gctcagcgcc agtccatgtg
     7141 cccgcagata gaactgcagc atccagttgt agcgcgacgg ctgcgtgtag gtgacggtcc
     7201 agatcgacga gagatgcgcg atccgcaccg gcagcccacc cgactgcagt gcctcgttga
     7261 attgctgcgc acgcgcgtcc caccgtgcat cgatgccgtc gtacatcgac ttcacctgcg
     7321 ggccatcgag ccgatcgaga aaggcgccca tcgcgcccat cacgcagggg tgtgcgttga
     7381 aggtgccgcg ggcaaagcag acgtcggccg gacggtcttc ccgaaagcgc ttcatcagcg
     7441 cagcgcgccc gcacacgacg ccgaccggca gcccgccacc gagcgtcttg ccgtacacga
     7501 ccatgtcggc tgcgacgccg aagtactgct gggcaccgcc gggtgccagc cggaacccga
     7561 cgaagacctc gtcgaagatc agcacgatgc cgcgctcggt gcacacctcg cgcaatgcgc
     7621 gaagccaggc ggtgtaggcc tcgcgatcga agcccgcgcg ccggccgctg tcgaccagcg
     7681 acgaatcggc cggtgcgccg gaattcgggt gcagcgcctg cagcggattg accagcacgc
     7741 aggcgatgtc gcgccgggtt cgaagcacct tcagggatcc ctcgtgcatg tcgcgcagcg
     7801 tgtaggtttc gcgcggtggc agcgggttgc ccgggccggg ctgaacgtct tcccaccagc
     7861 cgtgatacgc gccgcagaaa cgcaccagat tcttccgtcc ggtgtgatac ctcgccagcc
     7921 gaacggcctg catcaccgct tcggtgcccg acatgtggaa cgacacttcg tcgagacccg
     7981 agatggcctt caggcgctcg acgttgtcga tcacgcaggg gtggtaggca ccgagcaccg
     8041 gcccgaggtc acgcacccgc gcgacccctt cgtcgatgca ctccttgtag aagtcgacac
     8101 cgaacaggtt gacgccgtaa gaaccggtga ggtcgtagaa gacgttgccg tccaggtcct
     8161 ccaccgtcac gccatgcgac tccttcagga aggtgccgct cctgaggtgc ttgcgcaggt
     8221 aggggctgta ctggaacgga acccggtagc tgctggtgaa ctgcaggtcc gacacgctgt
     8281 cgcgcgcccg cgccgtcatg gcggcgctgc gcgggtagcg ctcgtcgtac agccgcgaca
     8341 gccgctggaa gccggctcga cgacgcgcga ccgcctcggc cgcggcgcca tccgacccga
     8401 agaagccgtc ttcgtcgtag gcgtagcccg gaatcagcga ggcgatgcgc ttggccatgc
     8461 gggagtggcc ggccagcgag cggtgcttgg ctttcgacag ttccatgcgt cgcttggcgc
     8521 gcggaaatgc ccaggccagg atgccgaggg tcagtgcgga gagtgcgaag gattcgtcca
     8581 tgagcaggtg tgttgatgtc caacccgtta tctaacagtc tcgattgaca ggaacgtgaa
     8641 aacaatgaat ccactgcgca gtctcgttgc caacgaggac ctcaacttcc tgctgaccaa
     8701 ccgggtgcca cgcatgctgc tgacccgctg gatgggctgg ttcagccaga tccgcaaccc
     8761 gtggatcgca cgcgcgtcga tcgccgtgtg gcgcctgttc accgacctgg acctgagcga
     8821 tgcccgcaag cagcgcttcg ccagcctgca cgactgcttc acgcgcgaac tccgtgacgg
     8881 cgcacgcacg atcgatcggc gtgcggacat cctgagcagc ccgtgcgatg cgatcgtcgg
     8941 tgcctgcgga cgggtcgatg acggccaggt gttccaggcc aagggcttcc cgtacgccat
     9001 ccgagatctg ttcggcccca gccaggacac cagcgccttc cgccgcggca cctacgtgac
     9061 cctgcgactc acatcggcca tgtaccaccg ctttcacgca ccgcaggatt gcgaagtcga
     9121 gcacctgacc tacctgagcg gagacacctg gaacgtcaac ccgatcgcgc tggcgcgggt
     9181 ggagcagctc ttctgccgca acgagcgcgc cgtgctccga ctgcgactga aggacagcgg
     9241 acacacgatt gcgctggtgc ctgtcgcggc catcctggtc gccagcatcc ggctgcattt
     9301 cctcgacgtg ctgctgcaca tgcgctaccg cgggtcggcg gagatcgcct gcagcgcgcg
     9361 cttcaccaag ggccaggaaa tgggctggtt ccagcacggc tcgaccatcc tggtctttgc
     9421 cccgcccggg ttcagcctgt gcgacggcat cacccccggc cagcacatcc gcatgggcca
     9481 ggcattgatg cagctcggtc ggcgcggatg acctgaagca gcgagcgccc gcgccgcggg
     9541 cttcgccggc acacccgacc gcggcgaacc ctcgacgccc tcaggtgcgc ggcgcgatat
     9601 cggcgcagtg gaccggcgac acggcccccg gcatcggcaa cagtgccgat tcggtccgca
     9661 ccggagccac cgcgacctgc cgtgcctcgg cactgcggtc gatgatctcg agccggccgt
     9721 cgtggtgctc gaccagcgcc gtgaggctct cgacccagtc gccgtcattg cagtaaagca
     9781 cgccctcgat ctcgcggatc tcggcgtggt ggatgtgtcc gcagacgacc ccatcgaggc
     9841 cgcggctgcg cgtctcgcgc gccagcgcgg cctcgaaatc gcccacgtag ctgaccgcgc
     9901 gcttgacctt cagcttgagg aatcgggaca ggctccagta cggcagcccg agccgcgccc
     9961 gggcgacgtt gaagcgccgg ttcagcctca gcgtgaattc gtaggcgatg tcgccgacat
    10021 aggccagcca cttcgcgcac tggatgacac cgtcgaagag gtcaccatgc gtcacccaga
    10081 gctggcgacc gtccgcggtg acgtgaacgc agtcctcgac gatgtcgacg ccaccgaagt
    10141 tgtgcgccac gtagcgccgc gcgaattcgt cgtggtttcc gggcacgaag atgacacgcg
    10201 tgcccttgcg tgccttgcgc aggatcttct gcaccacgtc gttgtggctc tgcggccagt
    10261 accaggagcg gcgcagttgc cagccgtcga tgatgtcgcc gacgagatac agcgtctggc
    10321 aatcggtggt tcgcaggaac tcgagcagcg caagtgcctg gcagccgggg gttccgaggt
    10381 gcagatcgga gatccacgcc gtgcgcacgt tcagggatcc atcctcttcg gtgttgtcca
    10441 tgcttgaggc tgctgtgtgc atggactcgc atggtcacgc cgtgacatga cacggtgttg
    10501 acacagaact cgcctgccgc cgaaaccggg caggaagctc cggcgcaaca cgctgtcacc
    10561 gtcccgtcag caactcttca cagacgtgtc atcggtcaca acgagcatct ccgcgaccca
    10621 aggagagacc gatgagggag caccccctga acaccctgcc gatgttcgac acgcacctga
    10681 acatgaaccc gtcgcgccat gcggcgcgac tgcatgtgcc gcacgcgccg accgtgtacc
    10741 gccagaaggc cgcggcagcc cccgtctcgt tcgaggccgt atgggcacgc gacctcgatg
    10801 aggtgcgcct ggcccagcag cttcgtcacc tcgtgttcgt cgaggaaatg ggcgcgcgcg
    10861 tgtcgctgcc cgcaggcacg ccgcccggcc acgacgccga ccttttcgac gaccactgcg
    10921 agcacctcct gctgcgcgtc accaccacgg agggtgagcc cggtccggtg atcggcacct
    10981 atcgcgtgct gacaccggct gcggccaagc gcgtcggcgg cctctacagc gagaccgagt
    11041 tcgacctgac ccgactgcgc tcgatgcgcg atcgcatggc cgaactggga cgatcgtgcg
    11101 tgcatccgga ctggcgcacc ggcggcgcca tcctggcact gtgggccgcg ctggcggagt
    11161 tcatgaagcg caacgcgctg gacacgatga tgggctgcgc gagcgtcggc atgcgcgatg
    11221 gaggccatgt ggcggcgagc ctgtggaccc agctgcgcgg cacgcacctc gcacccatcg
    11281 actggcaggt gcgcccgcgg ctgccgctgc cggtcgagca gctgcagtgc gatctcgatg
    11341 tcgatccgcc cgcgctgatc aagggctacc tgcgctgtgg cgccaaggtc ctgggccccc
    11401 cggcctggga tccggcattc aataccgccg acctgccgat gctgatccgc ctggccgact
    11461 tgcctccccg ctaccggaag catttcctcg gcgagtgagt cggtctcgag agcttccgcc
    11521 gccgcgggca ggcgcagcgt ccgtcagacc aggcgaggcc cgaagcctcg cactgctgcc
    11581 tgctacatgc ctcgcttggc atgtgtcagg atcgtggcga cctccgtcga tacctgcgca
    11641 aatcgcgatc ctgcaccgat caactgctcg gcctgcgcat actgacccgc gttgatctgc
    11701 ctggccacac cggcagctgt ctgatgaaac tcggcgtgct tgtccagcag cgcaacaaaa
    11761 ttcggtcgag tgccccactg cacaccgccg gacccatgga tccacttgcc cagagggcat
    11821 tggtcgtccc ggcagatcgt gtcggcgtcc agattctcgc ggtctgcgat cgccttgcgc
    11881 agcttgacct tccattgccg atgcgcctcg atcgccttgt cgaaatcgaa gtcggcgtgt
    11941 gcaccggact cggcagcgac cgtcagcgcc atgcccacgg gggtcttgaa gcgtgccacc
    12001 tcatcggcca gcgtgtgagc ctggtccttc atggctgatg cagccgcagc ggtctgctcc
    12061 acgagcgcag cgttctgttg cgtcatgcga tcgagatcct gaacggcctg gccgatctgt
    12121 ccaatgccaa ggctctgctc gcgggccccg gttgcgactt cacccagcaa ctggtccaca
    12181 cgctgcgacg aagccacgat ctcatcgatc gtcgttccgg cactgcgcac gatgcttgtt
    12241 cccgtctcga cctgctcgac gctgcgatcg atcaaggcct tgatttcccg agccgcgtcg
    12301 gcgcttcgct tggccagcgc tcgcacctcg cttgccacca ctgcgaagcc acgcccctgc
    12361 tcgccggcgc gggccgcctc aaccgccgcg ttgagggcca ggatgttcgt ctggaaggca
    12421 atcccgtcga tggttccaat gatttccccg atctggttgg acgaattgcg gatgccttcc
    12481 atggtgtgga ccacatcgcg catcactcgc cctccatccg ccgccgtctt ggcgttgtga
    12541 cgagccatct cggaagcttc cctggtgtgc tcggccgtgc tcttcaccgt tgaagagatc
    12601 tcctccatgg atgcggctga ctcctccagg ttggccgccg cctgctccgt acgggcggac
    12661 aggtcggccg cgcctgcggc gatctcgctg ctggagtgga ctatctcgtc gctggagcga
    12721 cgcaccctca agaccatgcc tcgcaacgat tcctgcatgg ctctcaattc gagcatcagc
    12781 tgtgcggcct cgtcgccgcc ccaaggcgat ggtgaggttg tcagatcacc ggaggtcatg
    12841 gctcgcaggt gacgccgcgt ctcgcgcagg ccaccgtcca tcacgcggta gaagcacagg
    12901 aagagatacg ccgcgatgat cagcgaggcc gccacgacgc ctgaagtcca cgccgccttc
    12961 tccagtgcat cgctgcgcag gtcttcgatg tagacacccg atccgacgac ccagccccag
    13021 ggctcgaaac cgatcacata cgaaaccttg tcctggggct tgcttttccc tggcttgggc
    13081 cactgatagg caacgaaacc cttgccatgt tgtcgcactt cggccacgaa tgccttgaac
    13141 agggccaggc cattcggatc cttcatgtca ccaacatcct tgccattcag ttccggcttg
    13201 atggggtgca tcaggatctt tggctgcatg tcgttgaccc agaaatactc cttgctgtcg
    13261 tagcgcacag cctccagcgc gctgagcacc gttttctggg cctggtcgcg ggtcagattg
    13321 ccggctgcct cctgcgcttg cgcccagacg atcagaccgt gtgcgacttc cacttgctgt
    13381 cgtgtcgcgt ccatccgtgt ttgcagcgca tgctcatagg acgacatctg atcccatgcc
    13441 agtgctccca gcagtggcag cgtgaatgcc agggagatga tcattgcctt ggacaagaag
    13501 cgaagccgcc ggaacaggcg cactccgggc gcccacagac cgtggtaggc gaagaagcct
    13561 cctgcgttgt ccggtgttct cgtgacctga gtgggcgggg ctgacatcga tggaaacacg
    13621 gccgacatga ttcacttcct tttcgagact gatggacctg cgctcgccat cgcgtctgtc
    13681 gacagcaagc aggcggcttc attgccgcag ggttcccgtc agatatcggc agcaccaagt
    13741 tggtgcttta gcggatttgc cagcgcagac gtcgttctgc tcgggcgcaa ggactgaaga
    13801 ttcgagcccg tgttcccagg cgagccgagg caaatgcact ccatcggtgc ccacgcgtgc
    13861 agcagtcagg agagatgtcc gagccgggca acgcatccgg gctgaaactg tcaccttgcg
    13921 ccaaggtggt gcaggaatgc tcaggcagga attcgagggc cagcgacccg atccgggaat
    13981 tacggatgtc ccttgccgat cgtgatgtag ccatcgatgc cgtcgtgggc cagcacgtcg
    14041 ttggcctcga acacggctga ggcagggggc atggtcacgc cggcggcctc acccgcggtc
    14101 gtactggctc cggcgaatgc ccagcggccg gtctccgctt cgcgccacca gcgcgggttg
    14161 cggtggcggt cgagcaggaa gccgagtccg ctgcgatcca ggcccagcca ctcgcagaac
    14221 ttgtccaggt gctgaggagc ggcctgctcg tggcggcgca ccagcgccag accctgcgcg
    14281 cgcgtcagcc gcccgtggcg gatctcgcgg caggcgtggt cggtgacctt cgagtagccg
    14341 tgcttgtgca gcttcagcac gtcgtgcagg tccatgtagt tccagcagtc gacgtggtcg
    14401 taggtgtcga aagtgcgggc gaacgcggcg gtgcggtagc cgcagcgctc gatcatctgt
    14461 tcgtgctggg ccttcggatc ccagcgcacg tagttgccga ggtagacgcc gcgcacgccg
    14521 atcgcggaca gcgccgcatc gtccgggtag cggtactgcc agatgtcgga ctccttcagc
    14581 gtgtcgaaca tcgtcggcag gtcgtcggct tcgcggccca tcaggtcgtg gtcctgccgg
    14641 tgacggcgcg tcatctcgac ctcgtcctcg tgcgagtaca tgccgacctg ctccagcccc
    14701 tggtgcgcgc cccacaggat cagcgggatg cggtagcgca ccgccgtctg caccgggaag
    14761 acggtctggc cggccaggca gtgccagtag tcgctgccga agtcgcgcag cgtcgcgcgg
    14821 gtgatcttct tcaccgcgtg cgggttgacg ttctgctgga tcaggtcgca gtcgaagcgg
    14881 atgcgcaggt tcgccaggtt gcggatgccc agcgaggtgt tgaagtactt gttgtaggtg
    14941 accagcagcg ggttcagtcc gagccgctgc ttgaccaggt ggacgatgta gtgcgaatcg
    15001 cccgcgccgc tgacgggcac gatgcagtcg taggtctcgc cgctgcggct gcggtacggc
    15061 gccaacagcg attcgaggcg ctgccagcgc tcggcccagt cgaggcggtc cttctcctcg
    15121 tgcacacggc agccggagca gatgccctcg gcgtcgagca tcagcccgag cgggtgcgcg
    15181 ctggtgtaca ggcagcggcg gcagtgcatc agtgcggcca tgtgttcagc tccaggcccg
    15241 cgcggggtcg agcggcgcat gcgtcgccgc gcgcaacggc agtccggcca atgactgctt
    15301 caggcgctgc accgcgtgct cgcggtggtt gaggaaattg ccgacggcgg ctgcgacgac
    15361 gttcggccgc tccagcacgc cgcgcagttg cgacggctcg ctgagaccac cgaacgcgat
    15421 cagcggcagc gtgggcatcg gccacagcgc cagcaggcgt tcatcgaaac cttcatgcac
    15481 gccctcgtgc tgccagtcga ccagcagcac ctcggagatc gcgcccgagg ccagcacgtc
    15541 gagcagtgcc ggcgccaggg gtgtcgagag acggcttcgg tggtccagcc aggccagcgc
    15601 cccgttgtgc accgacagtg gcagcaccgc gatcagcgcc tgcgcgccca gcggctcggc
    15661 gagttcggcg gccagatgcg ggtcgtcgtg cagcagcgca tcgatgccga tgcgttcggc
    15721 gccggcctgc actgcgcgca cgccgtcgga gacactgcgc accccgccgg tatagaccag
    15781 cggcgtcgac aggccgcgtc ggcacaaccg ctcgagcagc gccagatccg ggccgccgcc
    15841 ctgcgcgtcg cggtcgatgc actgcagcag gatctcgtcg acgccccagc ggtcgaggtt
    15901 ctcggcgatc acttcgggcc gcccgagcgg ccggtgccga cggtagccga tcgattgcac
    15961 cgcccagccg tccttgacgg taatgacgcc aatcagtcgc ttctcaagca cggcacaggc
    16021 cctcgatgac gttcttgagc agcaggcgac ccgccagctg gctcttctcc gggtggaact
    16081 gcagaccgac caccttgccg cgccgcaccg cgacggtgaa ggcgtcggcc agtcgcgata
    16141 cgccgatgca atagggcggc ggcgtatcga acgcatacgc gtgattgaag tacagcgact
    16201 gcccgtcgct cggcgacagc agcgcatcct cgggatcgat ctcgatggtg ttccagccga
    16261 tgtgccagcg tgcgccagcg agcggtcgaa cgcggcccgg gatcagcccc agtccggcgg
    16321 tcaggcggtg ttcgagcgac atgtcggcca gcagctgcat gcccaggcag atgccgatca
    16381 gcggttgtcc gctgcgtgcc tgctgtcgca ggaaatcgac cagcccgtgt tcgtgcaagg
    16441 acgccatcgc cgccggaaac gcgccaacgc cgggcagcac gatggcgtcg gccgcggcca
    16501 gcgtttcggt gtcgcgggtc acgcggcagc ggtggccgag accggtgatc gcgcgcgcga
    16561 ccgacgcgag attgcccgcg ccgtagtcga cgacaccgat cgtcagccgg gcgccgctca
    16621 cagccccacc cccacctgga aacggcggtg gatgcggccg tcgccatcga tatcgaacag
    16681 cgtgcgattg gtcgcggcat gcacctgccg ccagaacgcg tcgacgccga tgccgaggta
    16741 gtcgcagtag cgcgcgatgt agtccggcga gcaggcgtgg tcgtgctgct cgaccagttc
    16801 gatgcctcgc tcgcgactca tgcggccgag gcggatctcc tcgttgacgt agtcggtcgt
    16861 gcggccgaaa ccgaacttga agtacttgat catctggttg atcggcgtga agtcctcgtc
    16921 cagcgcggtc acgccgaaca ggtcgccggt gtcggccacg ccatcggtgc ggatctccag
    16981 cccctgcgcg cacgagtgca tcgcgttgtt gaccagcgac cagtcgccga ggaaccagcc
    17041 caggtagatg atctgcaggc ccagcgcgtc gaattcctcg ggctgcgggt agacgtaggg
    17101 cagcagctgg cggcgctcgt gacccgcgtc gagcatccac tgcaggccgc tcgacagcgt
    17161 gttcatgtgg cgcaggttgt tgccgtcgta gccggtcttg cccagcgtct tcaggtcgcc
    17221 gagctgcagc gccgggttct cgccccacag gatcaggtcg atgccgtagc gcagcgcgat
    17281 ctgcggcacc gccgagaaca gcgccagctc ggtcgagcgg aacgagttgg tgaaacgctc
    17341 gaagccctgg cgcttcagcg tgcgccaggt ggccggtgcg ggctgcgaga ccagcacgtc
    17401 gaagcccaga tcgatcaggt tcgacaggtt gtgcatgcca cggtcggtgg cctgctcggg
    17461 cggatagccc aggcacacca gcagcgggtt gacgtcgaat ttgtcgcgca cccacagcgc
    17521 ctggcgcgtg ctgtccttgc cgcccgagac accgatgatg caatcgtgga actggcccgg
    17581 cttgcggcga cggctcgcga acaggtcggt caggatgtcg atgcgctcct gccagtcgac
    17641 gccgcgcacc gcatcgacgt agtcgcatgc cgggcagatg ccggcatcgc tgaagcgagt
    17701 gttcgggcgg gtgtcgggct gcaggcagcg caggcagtaa cgcatggtga agtgaggcgt
    17761 cagtcggctg tcaatcggca aagcggaaac cctgctcgcg cgccagcgcc tgcatctcgg
    17821 gcggcacgcg gtaggtgttc aggtaggtgt agtccttgcg gcgatggatg aactcgctgt
    17881 aggccgccgc atggtggttg tcgcgcgccg caatgaagaa cagcgcgagc ttggcgccgt
    17941 agggtgccgc gcccgaggtc ggtgtggcgc ggtggtcgag ccgcgtgtga aacacgacca
    18001 gatctccgcc acgcgagccg agcaccgtgc cctgctgctg cgcacggtcc tggaagcgct
    18061 gcgcagcacc gggctgcccg gcggcgggct tcggaacgag gtggctgccg ttgacgactg
    18121 acacgccgcc gccatgctcg gcgctgttgt cctgcaggta gaccgcgcac tggtagatcg
    18181 cgtggtcggc gctccagtgc gccatgctgc cggcgtactc gaacatgtcg gtgtccttgt
    18241 gccagccgcc gaagccgtcg cggtgcgctg catgctcggg cagcagcacg aagcccggcc
    18301 cgatcagttc ggtcagcact tcggcgatgt gtggctggaa caggcgtcga tagatgcctg
    18361 gaaacctgaa gagatcgagc agcacgccgc cgcagccggg cggcgtcagg atgctgccgg
    18421 catgcttcgg cgaggagaag acatcttcga cctcgtggcg cagcgcctga accgcatcgg
    18481 gaccgaagac gccgcgggcg gcgtaccagc cctgacgcgc cagttgcagc gccggatccc
    18541 ggctgctggt gcggctgatg acgaccaccg ggtcgcgggc ggcgggccga gaggcgagtg
    18601 cggtctgcat gatgggcgga tctcagcgct tgcgtgcgat cgcacgcagg ttgcccgaca
    18661 ggccgtggtc agccaggaac tgctggaacg gcgccgcgag gcgcgcgtgg tcggtgacca
    18721 gcagttgctg aaggaacggc tgcccggaca catcgcagcg accggcctgc acggcctcgc
    18781 gcaccagttc gacatcgagc cgtcccgggg tggcgacttc gagcacctcg cagtccagcc
    18841 gctcgaggag gcgggcgatc gacgccgggt tgaagagatt cacatgctcg gtgtccaccg
    18901 cgaccgactc ggcgccgagc acctgggtgt cgaagccgtt gccgttcggg caggtcagca
    18961 ccagcagccc gccggtcttc agcaggcgca gcgcgcgtgc gaggaacggc accgggtcgt
    19021 acacatgctc gatgacctcg aagcacacca gcacatcggc cggatcgtcg aagcgctcgg
    19081 gcaggtcctc gacggcggtc tcgtgcacga cgagcccgtg gcgacggcag gcggcggcca
    19141 tttccggagt gcgttcgacg accgagacgc gctcgaacgc gccgctgtcg cgagccagcg
    19201 ccgcgaaggt accgaagccc gggccgagtt cgatcagatg gcccggccgc acgccatggg
    19261 cggcacaggc cgcggcgatc gtcgccagca gcggacggca cagcttgtcg cgacgccgct
    19321 cctcggacgc cgggaagatg tgctcggccc acagccggta gttctcggaa tggctgtagt
    19381 agtcgtccat cagcgccggc gtcggtcgcg gcgacatgta gagcgtgtcg caatcgtggc
    19441 agcgcacgaa ctggcagcgg tacttgtcga aggcgcggct cgcggcggtc tgcccgcagg
    19501 acgggcacgg taccgcgacg aaatcgccga ggtgctcgtg cagccgcgcg tggtcgcggg
    19561 ccatcgcttc gcgttgccgc gccatgaact gcggtccgcg gatctcggcg atgctgagtg
    19621 tctcgggcgt ggtcgtcatg ggtcgttgtc tctgtcaggt ggcgagcacg gcgctcgcgg
    19681 gatggaccag cgaccagctc gcgggcgtgc cgcgccgcac gtcctggcaa acgctgcagc
    19741 cgagcagcgc atccagttca cgcggcggca gcccgtagcc gggacggatg gcgcgcaggt
    19801 tggcagcggt cagcacgtcg ccggccttca ggtcctcgac gatgtagagc gagcggcggt
    19861 agcgcatcga tgcggtctcg gccaccgaca tgccgaagct cggcgcgccg agcgcctgcc
    19921 acgcacgctc gctctcgatg cgcagctgtg cgaactcggc gggctcgagc gagaacgccg
    19981 aatcgacacc gccctcggcc cgcgactcgg tgaagtgctt ctcgatgacg accgcgccga
    20041 aggccaccgc cgccaccgcg gcaccgcagc ccatcgtgtg gtcggacagg ccgacttcgc
    20101 agccaaaggc gtcgcgcagc accgggatgc tgcgcaggtt ggtgttcagc ggcgtcgaag
    20161 ggtaggtgct ggtgcacttg agcagcacca ggtcgcggca gccggcggca cgcg
//
LOCUS       DAOORV010000062        20031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3288_length_20031_cov_7.344864, whole genome shotgun sequence.
ACCESSION   DAOORV010000062 DAOORV010000000
VERSION     DAOORV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_20031_cov_7.344864"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKV56_09865"
     CDS             <1..1101
                     /locus_tag="PKV56_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX68159.1"
                     /translation="ADANAADKSRKLLAPKGLDALRADLDAAQARESEATSALAQLPP
                     EPTEAALPLDAAEREHETARVAAEVAGQQLQAARQALATAASQHDAAKQERDTLKVVL
                     DDPKLQAELAAKRLELLEAGTRESIVKDEVDAIVARIAAARPDILTQDADRFRRSAEE
                     AENQHRARDRQIVQVETALAAAGAQGLEEELERALSASQLAQRRAAELRRRAEALDHL
                     LQKLEQRRQALTRRLQAPLQKHLDRYLQLLFPSGRLAVDDNLVPGALTRPGTRGPDAA
                     DFETLSFGAREQMGVISRLAYADLLKEAGRPTLIILDDALVHSDEQRLAQMKRVLFDA
                     AQRHQVLLFSCHPGRWRDMGVPVRSLEAERSQ"
     gene            1098..1850
                     /locus_tag="PKV56_09870"
     CDS             1098..1850
                     /locus_tag="PKV56_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68160.1"
                     /translation="MSGRVKIGTWNLERSGAHHRGRMAGQLACLAERDCDVWVLTETH
                     DDLRPTGYDYKVDSATSPRWQSPGERLVAIWSRHPLTVVQTDDPCFTVAARLELPGYP
                     RGLLVYGTVITYAHDTASPAGPAPAPVWSRHRAAVAAQTQEWMRLRARFAADDMCIAG
                     DFNMNLDGTRWYGVRDAREALLKGLRAAGLRCLTTEDLRATRPDIGRASVDHICFSDV
                     PGATAVVDAWSGGQGKSRLSDHNGLAVEMSLP"
     gene            complement(1954..2757)
                     /locus_tag="PKV56_09875"
     CDS             complement(1954..2757)
                     /locus_tag="PKV56_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68161.1"
                     /translation="MTVHSCGKPIAHINGRTYKDALHELGGDERTIRRDQLTDWVAVC
                     PRCDRLKLGATRPVGWPFRDADGVMRTVHDHDGLELRACCETMSFGGFLFSRRAFEAG
                     VHLARTGDPTTANVEALGRDFLARPTTEAARAFCEAVCIWGRGQRVLGKLSAFHGDEL
                     GPYVHDWLVRVPDLDPLDAVAAGIEIKGLAVSFASKHLRLLQPERFPVLDEVLEAGLG
                     IARNPAGYRLFTHMLSDFLAEHQLQETIAVAEGAIFMLVRQGVRAVDDN"
     gene            complement(2822..4816)
                     /gene="recD"
                     /locus_tag="PKV56_09880"
     CDS             complement(2822..4816)
                     /gene="recD"
                     /locus_tag="PKV56_09880"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOX68162.1"
                     /translation="MTPTEMLHRLDDWRDSGWLRRLDVAFARFIHELDADAPASLLLA
                     AALAAHLEGRGHSALPLAELAASSDALLCWPLEGGAALRQAWAEHMAHSEAGAPPAAW
                     QSRSVLEIDPEDDTGASPLVLCRGLLYLRRYWRYESRVAVQINARAGQGTEPGETAAT
                     AIDRAKARHLLDDLFAAAGPEVDWQKIACALALRSHFTIITGGPGTGKTYTAARLLVL
                     LQGLHAGAQPLRLAMAAPTGKAAARLRQSIQKALHGLRESIGESVGIGEWADQLVPAR
                     TLHALLGTLPGTRRFRHDATNPLDVDLLIVDEASMVHLEMMAQLLEALPAGARLVLLG
                     DKDQLASVEAGAVLGDLCQGTAADDDAHGYGTETAAWIRDVAGQALPPPTRSQGAGLT
                     QQTVVLRKSRRFDGPIGQLALAINAGNAASAVEMLEARADGPIAMRLVASAGAVLPVA
                     IDGRDGAPGGYRAYLEHVSQGPASGEAFDDWAKAVLSTFDSFRVLTAVREGPWGVAGL
                     NSAIERALSEAGLLQRRGEWYEGRPVIVTRNDASLGVFNGDVGVVLRGPTGDSALRCF
                     FLDGDELRSVAVGRLADVETAFAMTVHKSQGSEFAHVLLVLPDEDARVLTRELVYTGI
                     TRASAAFTLMGKSTEQLTLATHRLTRRVSGLRQLLAKDKE"
     gene            complement(4813..>6731)
                     /locus_tag="PKV56_09885"
     CDS             complement(4813..>6731)
                     /locus_tag="PKV56_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX68163.1"
                     /translation="DTVWQTPEAADLLRLLQAVASPRDVRLARAALATTLLGHTMAEL
                     VALASDDVLFDRQCERLQQLHHTWKAQGVLAMVRRALHAFELPARWLAPNDPNQQGER
                     RLTNVLQLAELLQAASAQAEGEQALIRWLAQQIADAAEGLNGSDDAVLRLESDADLVK
                     VVTVHKSKGLEYPLVFLPFAAHFRELITAGESAKDAVLLLPGAGGADGGPGGLGGRVL
                     VVSPTQAQLTVAETERQREELRLLYVALTRARHALWVGLSGLQVGTGKSYVWHRSAIG
                     YLLSGATEQPPEQRIQDVLAFATSSQGVVVEQVPAAEGLPLPSVTALQRRQTLPDLAQ
                     PPVYNAQFDREWAISSYSALVRDAAWGPSGSGSVPARVVRDDEPGEAVAAAVPGNPGG
                     QAWHRFPRGALAGNFLHDQLEWLAAERFALDGEDDLQRALVRRCERQGWGHRADDVVA
                     WMRQVCTTPLPALGVPLAALSTLAPEMEFWFPSDGLQAGLIDSLCRQHVQTGRARPTL
                     PERELKGMLMGFADLVFEYEGRYWVLDYKSNTLGSRDADYTHEAMESAMLEHRYDVQA
                     AIYLLALHRLLRTRLGTAYAPENHLGGAIYLFLRGMHSPTAGCCHVAPPLALLNELDG
                     MLASGARATTGEAA"
     assembly_gap    6732..6831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6832..8699)
                     /locus_tag="PKV56_09890"
     CDS             complement(<6832..8699)
                     /locus_tag="PKV56_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX68164.1"
                     /translation="MSEPLDPLNLPLRGSRLIEASAGTGKTWTIAALYVRFVLGHRCE
                     ARLPAEILVMTFTRAATRELSDRIRRRLVEAAACFRGQPVPADDAFLRSLLQDFPAGP
                     AREQAAWQLSVAAEAMDDAAVHTIDAWCQRMLREHAFDSGCLFDEELQAQESAMRAEA
                     VRDYWRQQVYPLDDAALAGVLAQWEGVDALAKDLKDLHDKPLPVDAGQGTLLDAATRA
                     IQRRAEQLAELKHGWAERADEMLGWLRAQWQRKDCPIDKKKLGEANGTKWLQALKGWA
                     SDAAADVPKLGVGAERLTRQGVLGSIKPGMSAEVPDVFGGIEALMDALIALPEVAPAL
                     RLHAAAHVQGRMQQLKERAGTFGFADMLNRLDVALDESLSGASALRLRQRILAQHPVA
                     LIDEFQDTSPVQLRIFDRLYRIADDDASRALLLIGDPKQAIYGFRGADIYSYLGARQA
                     TAGRHHALGTNHRSTQALVGAVNGLFGAAEARAGEGAFMFGAAATASALPFAPVQARG
                     RAERLVASGQEMAALTLTFDEPLRNKEDSRSLFAALCAERIVTLLNDPQVGFAEEGEP
                     FKRLRPADIAVLVRSRTEADVVRREMRRRGLASVYLSDKDTVWQTPEAADLLRLLQA"
     gene            complement(8692..12243)
                     /gene="recC"
                     /locus_tag="PKV56_09895"
     CDS             complement(8692..12243)
                     /gene="recC"
                     /locus_tag="PKV56_09895"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOX68165.1"
                     /translation="MGDEKQAGLLVLHGNRLELLAEAVFTWLASNPLAPLEQETILVQ
                     SNGMGEWFKMELASAHGVCASTTVELPARFIWRAYRAVLGRNAVPGAAPLDKLPLTWR
                     LMQLLPRLADQPGFEPVRGFLADGDASRRLQLAQRLADMFDQYQVYRGDWLEDWGQGR
                     DLIRKGGQGGARAADAVPLDQLWQPALWRELQAALTPAERDLSRAGTHRRFLDALERP
                     QVSGAKAFPELPRRLVLFGTTHIPHQTLEAIAALAAHCQVLMAVPNPCRYHWADIIEG
                     RELLRFERRRLPLRRDQDLSAVPLEQMHAHGHPLLAAWGRQSRDFVRQLDAFDDVQAA
                     RQRFQLPRVDLFDEAPGTTLLQQVQAAVRDLLPLGEHTGQGARLDDRSIVFHVAHSAQ
                     REVEILHDQLLDLLANPPGGQALSPRDIVVMVPDIDTFAPAIRSVFGQYPRWDARHIP
                     WGIADQKDRGHQPLMVALEWLLRMPQERLGASQIRDLLDVPAVASRFGVQPEDLPTLA
                     QWIEGAGVRWGLDAAHRDSLGLGAGGEQNTWAFGLRRMLLGYATGELAGAPGFAGIEP
                     YAEVAGLSAGLAGALADLLHQLADWWADASMPRTPQGWADRLRSLLADVFAATDDAER
                     AILAALSDALAQWLQACEAATFDEPMPLAVAREAWLDTVDEPGVSRRFKAGGVTFCTL
                     LPLRAIPFQVACLLGMNDGDYPRRGSRSDFDLMALPGQAQPGDRSRRDDDRQLMLDAL
                     LSARRVLSVSWAGRSPRDNQEQPPSTLVAQLRDYLASGWGAEVLAQRTTEHPLQPFSR
                     RYFEPSAAGDPGRLFTYAGEWRTAHDDRTPSAVPVGSAASAEGAALGERALRPLTVQG
                     LAAFLRNPVKEFFRHRLQVSFSEIDVVDEDDESFGGDKLERWALLDDVLRACRGWTEA
                     PAEAPPPLPDFLAQQVARLQRAGRLPMAGPGRRTQDGLLQTLLPVAAAWQQVLAEHPF
                     VREKQPMRLVHPQDDRLVLDDWLVGLHAADAHEPPVWIELQASKVADAGTKKSGPIPR
                     ADKFLTAWVRCLASAACGCPVDGIVIGEGAMVRVSPPAQAEAMATLTALMQTCRDGLG
                     GDSPMPTALRTGVAWLSKPESARAAYEGQFRSPVPGEGQEACLARIFPDFASLAGHLD
                     FDAATQRLYEPYRQWLADHVTVTMLNNETAEQGAGDE"
     gene            13029..13472
                     /locus_tag="PKV56_09900"
     CDS             13029..13472
                     /locus_tag="PKV56_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX68166.1"
                     /translation="MTSQTDLPAALQTKERYSIGELSTLTGVTPRTVRYYIAQGLLER
                     PQGEKRGAHYLRRHLEQLLLVRRWTEAGLSLDRVRELLDGAPEDPPRRAAAPGSVEVW
                     SRITVADGLEVHLEPGRAGLSPEQVRALVRSVTGLYRQVRSGTQS"
     gene            13525..15813
                     /locus_tag="PKV56_09905"
     CDS             13525..15813
                     /locus_tag="PKV56_09905"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HOX68167.1"
                     /translation="MEIESRAFALSVSDPGAPAPVLTGVTASGRLDGVLFNLALRQTY
                     RNASDRNLEVVYTFPLPCEAVLLAFASELNGERTEGVITAKREAERQYEEALLKGDAP
                     VMLEALDGGLYTANLGNLKPGEEIVLDVRFAQLLSFEQGRLRLAIPTTVAPRYGNAES
                     AGLLPQQVPTASLDAEYPLSLSVLVSGSLSGEDIQCPTHKFTRTEVEDGVRLDLLPGA
                     SLDRDVAIIVTPRETRPSLVVQARDTRSSAAPVVLMAAFQPPATASRKLISLKLLVDC
                     SGSMGGDSIASAKLALRSVLAHLTDSDRVSLSRFGSTVEHILASTSYSASALAHLSSL
                     VDAIQADLGGTEMEAALSSVFELGSGNEGIDSDVLLITDGEIWQARETAAAAKASGHR
                     VFAIGVGSSPAEAVLRSLAESTGGACEFAAPGESLEAAARRMIGRIRQVPWQGVFVDW
                     GQTTLWEVQPPTSVFGGDTVIAIAGMAAAPTVSQARLMSKPKVGENVELARGEAQAPC
                     PGDSLARIAAARRIAGTEGEPSLSLAIEYQIMSKQTNCILVHQRADNDRATDEAELRR
                     IHSMLAAGWGATSTRLSAPSLMCQDVSGLSSPMLWRSTAAFGSADVKLSVIDSDDHHI
                     PAFLRKQADHQRPASLRTFALAVVEHMANGGQVQGLPSHCAYLDIHPDLHDAMLQVRG
                     LGVSEGVAMLLLANWVNAREDGLKSAFVEASLAEQLRTVDASLQVRCSELFERLLGQY
                     GLDGWSSSRTHRLRKAMARVLP"
     gene            15820..16632
                     /locus_tag="PKV56_09910"
     CDS             15820..16632
                     /locus_tag="PKV56_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68168.1"
                     /translation="MNFEQSPVNQPVPDSLPAAARGSHKHVLDWTSRPDFLREFSELL
                     ATPEVDVVGARYMPCGYGKPAEARLESFGPDVLPGHPAWGRVADWWLRHPKGANTPNW
                     DIAVTARIEGRPGLVLVEAKANVPELSRAGKPTSPGASKRSQQNHEQIGHAIDQANTG
                     WRSVAPEARLTRDTHYQLANRLAFAWKLASDGVPIVLLYLGFTGDDGIADAGEPLRDD
                     SHWRQLFREHLQELCVDGCLDRRVLFGDVPVRVICRSRPVLSVSPSRHFRRP"
     gene            16755..17669
                     /locus_tag="PKV56_09915"
     CDS             16755..17669
                     /locus_tag="PKV56_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68169.1"
                     /translation="MNQNKHLLESNAVDKSPFSSATLRWLTLALLLLVLSVAWLKPLD
                     ERARAETEAGLKRALVTYAVARTINGIVSVVQESTVSLQPGGLGVTLAPAQFLDPVND
                     LVEQFASVMLAVCVSFGIQLMLLNIGGSTAVNVALTLVVFLWLAARWREWTAPRLLSA
                     LVVGLVFLRFAVPLLAIASETTYRYVLADEYSAAQQKVEAFAEGARNTSATDSLSLAP
                     DQAGAQTLWERIKKLPDSVDIEAKLRRTQERFESVKSWLDRTVEHVVRLMALFALQTV
                     FLPLLFLWALVRLSSIAIGANRASRMAS"
     gene            17748..18806
                     /locus_tag="PKV56_09920"
     CDS             17748..18806
                     /locus_tag="PKV56_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68170.1"
                     /translation="MTQSSSSLDWFEKITGFREGTYDETRARLSIENGLLVSPFSQRR
                     CGVGQLELASLNDLRVHRGATSGSVRRTTVRCLAADVRALHSDPEFSGALFQVASQFN
                     LLEMVSPNVSPEDGVARYAHDRTQGPACAIAAGAATIYRNYFAPVGEGRGQTAVRQID
                     ALAPMGEALSHQLNRPVHALWTMRNGYAICTLDGLRSIGEYLRRSTEPTLDHLRAQLA
                     IGLHWRVEVTDSEQPTRPTVSQAFCSALPVRYTHIPTAAWEPFARLVLEAAYEATLLA
                     ACKQRNEGGSRTVLLTRLGGGAFGNDDRWIDEAIDRALRLVEFEGLDVCLVSFGRVHA
                     AFSAMDTAWNSGPASRHE"
     gene            complement(18940..>20031)
                     /locus_tag="PKV56_09925"
     CDS             complement(18940..>20031)
                     /locus_tag="PKV56_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68171.1"
                     /translation="AFGHRSWAAFHSESLVVDTGIGEAPKDSLPQVIGRAVQLQYEQN
                     AARAMAEALLNFAHERQLGALGRKALEESLRPPPRYVDEDGEDDEPEDWTEVAAKSVP
                     SWLPSRERLLGSSLLVSSLEQEAASKPERHLLLAALYRCGKPNPYLYDESLKGRVLTS
                     VERGWADEYLRLEPRYRKYEEHLKQAALGGVRAAALEYGKVFNRPEFFALAERLTGEV
                     DALEMARIASTSQSRASWLRKAAEQGSRSALEALADLGEAWAEERMAQQGDTGWMRGA
                     AERALAHGDALRAWTWHYLALEHGVDLTKSSMAAYHDGGENDGQFYDSDFGGPAYVSG
                     EVGLELPELNLSGHREARAKALEILERAS"
BASE COUNT         3483 a         6669 c         6363 g         3416 t
ORIGIN      
        1 gcagacgcca acgcggccga caagtcgcgc aagctgctcg cacccaaggg actggatgcg
       61 ctgcgtgccg atctggacgc ggcacaggcc cgcgagtctg aggccacatc ggcactggcc
      121 cagctgccgc cggaacccac cgaagctgcc ctgcccttgg acgcggcaga gcgcgagcac
      181 gagacggcgc gggtggccgc agaggttgcc ggtcagcagt tgcaggccgc tcggcaagcc
      241 ctggccactg cagccagcca gcacgatgcg gccaagcaag agcgcgatac cctgaaggtg
      301 gtgctcgacg acccaaagct gcaagccgag ttggctgcca agcggctgga gctgttggag
      361 gccggcaccc gcgagtcgat cgtcaaggac gaggtggacg ccatcgtggc gcgcatcgcc
      421 gccgcgcggc ccgacatcct gacgcaggac gcggatcgct tcaggcgcag cgccgaggaa
      481 gccgagaacc aacaccgtgc gcgtgaccgc cagatcgtcc aggtcgaaac ggcgctggcc
      541 gccgcgggtg cgcagggcct ggaggaggaa cttgaacgtg cactaagcgc cagccagctg
      601 gcgcagcgtc gtgccgccga actgcgccgc cgggccgagg cgctggacca cctgctgcag
      661 aagctggagc aacggcgcca ggcactgacc cgccggctgc aagcgccgct gcagaagcat
      721 ctggaccgct acctgcaact gctgtttccg tcaggccggc tggcggtgga cgacaacctc
      781 gtccccggcg ccctcacccg ccctggcacg cgcggcccgg acgccgccga cttcgagacc
      841 ttgagcttcg gcgcccgcga acagatgggc gtgatcagcc ggctcgccta tgccgacctg
      901 ctgaaggagg cgggcaggcc cacactaatc atcctggacg acgcactggt gcacagcgac
      961 gagcagcgcc tggcccagat gaagcgcgtg ctgttcgatg cggcacagcg ccaccaggtg
     1021 ttgcttttca gctgccaccc aggccgatgg cgcgacatgg gtgtgccggt ccgatcgctt
     1081 gaagccgagc gttcgcaatg agtggtcgcg tcaagatcgg cacctggaac ctagagcgct
     1141 caggcgcaca ccaccgagga cgtatggcgg gccagttggc ttgcctggca gagcgtgact
     1201 gcgatgtctg ggtgctgacc gagacacatg acgacctgcg gccaacggga tacgactaca
     1261 aggtggacag tgcgaccagt ccgcggtggc agtcgccagg agagcggttg gtcgccattt
     1321 ggtcccgcca tccgctgacg gttgtccaga cggacgaccc ttgtttcact gttgcggctc
     1381 gccttgaact tcctggctat ccacgcggat tgctggtcta cggaactgtg atcacctacg
     1441 cccacgacac cgcttcccct gccggaccag ctcccgcgcc ggtgtggtcc aggcatcggg
     1501 cggcagttgc cgcccagaca caggagtgga tgaggctgcg cgctcggttt gctgccgacg
     1561 acatgtgtat cgcgggagac ttcaatatga acctggacgg cacgcgctgg tacggcgtgc
     1621 gcgacgcgcg cgaagcactg ttgaaggggc tgcgagccgc aggcttgcga tgcctgacca
     1681 cagaggactt gcgagcgaca aggccggaca tcggtcgcgc cagcgtcgat cacatctgct
     1741 tctcggacgt gccgggcgct actgctgtcg ttgacgcttg gtcaggcggg caaggcaaga
     1801 gccggctttc ggatcacaac ggcttggcgg ttgagatgag cctaccgtga gcgagcaaac
     1861 cgaccacgca cgccgagatg agatttcctt aactggtcga cctcgaggtg ctgaccagga
     1921 tacggaagac cgctccgtgg gcctgcgaag cactcagttg tcatcgacag ctctcactcc
     1981 ctggcgtacg agcatgaaga ttgccccctc cgccactgca atcgtctcct gaagctgatg
     2041 ttcagccagg aagtcggaca gcatgtgcgt gaagagcctg tagcctgcgg gattgcgagc
     2101 gatcccaagc ccagcctcaa gaacctcgtc caagaccgga aagcgttcgg gctgaagcag
     2161 ccgaaggtgc ttgctcgcga acgacacggc gagacccttg atctcgatcc cggccgccac
     2221 cgcatccagt ggatcaagat ccggcacgcg taccagccaa tcgtgcacat acgggcccag
     2281 ctcgtcgcca tggaacgcgc tgagcttccc cagaacgcgc tgccccctgc cccagatgca
     2341 aacagcctca cagaacgctc tcgccgcctc ggtggttggc cgcgccaaga agtcacgccc
     2401 tagcgcttca acgtttgctg tagtcggatc gcctgtccgt gcgaggtgaa cgcctgcctc
     2461 gaacgcgcgt cgactaaaca ggaaacctcc gaaagacatg gtctcgcagc atgcgcgcag
     2521 ctccaagccg tcatggtcgt gcacggtacg catcacgcca tcggcatctc gaaacggcca
     2581 gccaactggt ctcgttgcac ccagcttgag tcgatcacag cgtgggcaga ctgcaaccca
     2641 atcggtcagc tgatcacggc ggattgttct ttcgtcgcct cccagctcat gcagcgcgtc
     2701 cttgtaggtt ctaccgttga tgtgcgctat tggtttaccg caagagtgga cggtcatgtc
     2761 gattgggtga agtgggtctc aatgggaaag acgagaggcg cggcgttggg gatccttcag
     2821 gtcactcttt gtccttggcc agcaactgtc gcagaccgct gacccggcga gtcaatcgat
     2881 gagtggcaag agtcaactgt tcggtactct ttcccatcag agtgaacgca gcgctcgccc
     2941 gggtgatacc tgtatagacc agctcccgcg tcagtactcg cgcatcctca tcgggcagca
     3001 ccagcagcac atgtgcgaac tcggagccct ggctcttgtg caccgtcatc gcgaacgctg
     3061 tctcgacatc ggccaggcgg cccaccgcca ctgagcgcaa ttcgtcgcca tcgaggaaga
     3121 agcaacgcaa cgccgagtcg cccgtcgggc cccgcagcac gacgccgaca tcgccattga
     3181 agacacccag cgacgcgtcg ttccgcgtca cgatgaccgg gcggccttcg taccactcac
     3241 cacgccgctg cagcagcccg gcctcggaca gtgcacgttc gatggctgaa ttcaggcccg
     3301 ccacacccca cgggccctca cgaacagccg tgagcacgcg gaagctgtcg aaagtgctca
     3361 gtacggcctt ggcccagtcg tcgaatgcct ctccggatgc gggcccttgg gagacatgtt
     3421 cgagataggc gcgatagccg ccaggcgccc catcgcgacc gtcgatagct accggcagca
     3481 cggcaccagc actggcgaca agtcgcatcg cgatcggccc atccgcgcgc gcctccagca
     3541 tctcgacggc tgacgcagca ttgccggcgt taatggccag tgccagctgc ccgatcggcc
     3601 cgtcgaatcg tcggctcttg cgcagcacca ccgtctgctg ggtcagccct gcaccttggc
     3661 tgcgcgtcgg cggcggcagg gcctgtcccg cgacatcgcg gatccaggct gcggtctcgg
     3721 tgccgtatcc gtgcgcgtcg tcgtcagcgg cagtgccctg gcacaagtcg ccgagcaccg
     3781 ctcctgcctc gaccgaggcc agctggtcct tgtcgcccag caggaccagt ctggctcccg
     3841 ccggcagggc ctcgagcagc tgcgccatca tttccaggtg caccatcgag gcctcgtcga
     3901 cgatcagcag gtcgacgtcc agcgggttcg tcgcatcgtg ccgaaaccgg cgcgtgcccg
     3961 gcagggttcc gagcagcgcg tgcagggttc gcgcgggcac gagctggtcg gcccactcac
     4021 cgatcccgac cgactcgccg atgctttcac gcaggccgtg cagcgccttc tgaatcgact
     4081 ggcgaaggcg cgctgccgcc ttgcccgtgg gagccgccat cgccaaacgc aagggctgag
     4141 ctccggcatg caggccctgc agcagcacta gcaggcgtgc cgccgtgtag gtcttccccg
     4201 tgccggggcc gcccgtgatg atcgtgaagt ggctgcgcaa cgccaacgcg catgcgatct
     4261 tctgccaatc gacctcagga ccggcagctg cgaacaggtc gtccaggagg tggcgagcct
     4321 tggccctatc gatcgcagtc gcagccgtct cgccgggttc cgtcccctgg ccagctcgcg
     4381 cgttgatctg caccgccaca cgggactcgt agcgccagta gcgccgcagg tagagcaggc
     4441 cccggcacaa caccaggggc gatgcgcccg tgtcatcctc aggatcgatc tccagcacgg
     4501 acctcgactg ccacgccgcc ggcggcgctc ccgcctcgct gtgggccatg tgctcagccc
     4561 aggcctggcg caatgccgcg ccgccctcca gcggccagca aagcagtgcg tcggagctgg
     4621 ctgccagctc ggccaacggc aaggcgctgt gtccacgccc ttccaggtgc gcggccagcg
     4681 ccgcggccag cagtaggctc gccggcgcgt ctgcatccag ttcgtggatg aagcgcgcga
     4741 acgccacgtc cagcctgcgt agccagccgc tatcgcgcca gtcgtcgagc cgatgcagca
     4801 tctcggtggg cgtcatgcgg cctcccccgt cgtcgcgcgc gcgcccgacg ccagcatccc
     4861 atccagttcg ttcagcaggg ccagcggcgg cgccacgtgg cagcagccgg cggtagggct
     4921 gtgcatgccg cgcaggaaga ggtagatcgc accgcccagg tgattctcag gcgcataggc
     4981 cgtacccaaa cgcgtacgca gcagcctgtg cagggccaat aggtagatgg ctgcttgcac
     5041 gtcgtagcgg tgctcaagca tggcggactc catcgcttcg tgcgtgtagt cggcatcgcg
     5101 cgaacccaag gtgttcgact tgtagtccag cacccagtag cggccttcgt attcgaacac
     5161 caggtcggcg aagcccatca acatgccctt gagctcacgc tcgggcagcg tgggccgggc
     5221 ccggccggtc tgaacgtgct gtcggcacag gctgtcgatc aggccggcct gcagtccgtc
     5281 gctcggaaac cagaactcca tctcgggtgc gagcgtgctc agcgccgcca gcggcacgcc
     5341 cagcgcaggc agcggggtgg tgcagacctg acgcatccag gccactacat cgtcggcacg
     5401 gtggccccat ccctgccgct cgcagcgcct gaccaacgcg cgctgcaggt cgtcctcccc
     5461 gtccagcgcg aagcgctccg ctgccagcca ctccagctgg tcgtgcaaga agtttccggc
     5521 cagtgcaccg cgggggaagc gatgccaggc ctggccgccc ggattgccgg gcacagcagc
     5581 ggctacagct tcgccgggtt catcgtcacg gacgactcgc gccggcaccg atcccgaccc
     5641 ggacggcccc catgccgcgt cgcgcacgag cgccgagtag ctgctgatgg cccactcgcg
     5701 gtcgaactgc gcgttataga ccggaggctg cgccaagtcc ggcagtgttt ggcgccgctg
     5761 cagtgccgtg acagacggca gcggcagccc ctcggccgcc ggcacctgct cgacaacgac
     5821 gccctggctc gaggtggcga aggccagcac gtcctggatg cgctgctccg gcggctgctc
     5881 ggtagcgccc gagagcagat agccaatggc actgcggtgc cagacatagg atttgccggt
     5941 acccacctgc agcccggaca gtccgaccca cagtgcatgc cgtgcgcggg tcagcgcgac
     6001 gtagagcaac cgcagctctt cgcgctgccg ctcggtctcg gccacggtca gctgggcttg
     6061 ggtcggcgac accacaagga cccggccgcc cagaccgccg ggcccgccgt ccgcgccacc
     6121 cgcgccaggc agcagcaaca cggcgtcctt tgcgctctcg ccagccgtga tcagctcgcg
     6181 gaagtgcgct gcgaagggca ggaacaccag cggatactcc aggcccttgg acttgtgcac
     6241 ggtgactacc ttcaccaggt cggcgtcgct ttccaggcgc agcacagcgt cgtcgctgcc
     6301 attcaggccc tcggcagcgt cggcgatttg ctgcgccagc cagcggatga gcgcctgctc
     6361 gccctcggct tgcgccgagg cggcctgcag cagttcggcc agctgcagga cgttcgtcag
     6421 gcggcgctcg ccttgctgat tgggatcgtt cggcgccagc caacgcgctg gcaactcgaa
     6481 ggcatgcagc gcccttcgca ccatcgccag caccccttga gccttccagg tgtggtgcag
     6541 ctgttgcagt cgctcgcact gccggtcgaa gagcacgtcg tcgctcgcca gtgccaccag
     6601 ctcggccatc gtgtggccca gcagtgtcgt ggccaatgcc gcgcgggcca gccgcacgtc
     6661 gcgcggtgag gccacggcct gcagcagccg cagcaggtcg gccgcttcgg gcgtctgcca
     6721 gaccgtgtcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcctgcagc
     6841 agccgcagca ggtcggccgc ttcgggcgtc tgccagaccg tgtccttgtc ggagaggtag
     6901 accgaggcca ggccgcgtct tcgcatctcg cgccgcacca cgtcggcctc ggtgcggctg
     6961 cgcaccagca cggcaatgtc ggccggccgc agacgtttga agggctcccc ctcttccgcg
     7021 aagccgacct gcgggtcgtt gagcagggtg acgatgcgct cggcgcacag ggcagcaaac
     7081 aggctgcggc tgtcctcctt attgcgcagc ggttcgtcga aggtcagggt cagggctgcc
     7141 atctcctgcc cgctcgccac caggcgctcc gcgcggcccc gggcctgcac gggggcgaag
     7201 ggcagtgccg aggccgtggc ggcggcgcca aacatgaagg ccccctcgcc tgctcgtgct
     7261 tcggccgcgc cgaacaggcc gttcacggcg cctaccagcg cctgcgtcga ccggtggttg
     7321 gtgcccagcg cgtgatgccg accggccgtc gcctggcgcg cccccagata gctgtagatg
     7381 tcggcgccgc ggaacccgta gatcgcctgc ttgggatcac cgatcaacaa gagcgcccgc
     7441 gacgcatcgt cgtcggcgat gcggtacaga cgatcgaaga tgcgcagctg caccggtgac
     7501 gtgtcttgga actcatcgat cagcgccacc gggtgctggg ccaggatgcg ttgccgcagg
     7561 cgcagcgcgc tggcgccaga caagctttcg tccagggcga catccaggcg gttcagcatg
     7621 tccgcgaagc cgaaggtgcc cgcacgctcc ttgagctgct gcatgcgacc ctgcacgtgc
     7681 gccgcggcgt gcaggcgcag cgcgggagcg acttcgggca gtgcgatcag cgcatccatc
     7741 agtgcctcga taccgccaaa aacgtcaggt acctcggcgg acatgccagg cttgatcgaa
     7801 cccagcacgc cctggcgcgt gagccgctcg gcgcccacgc ccagcttcgg aacatccgcc
     7861 gcagcgtccg aggcccaacc tttgagcgcc tgaagccact tggtaccgtt ggcttcgccc
     7921 agcttcttct tgtcgatcgg gcagtccttg cgctgccatt gagcacgcag ccagcccagc
     7981 atctcgtcgg cccgctctgc ccatccgtgc ttcaactcgg ccagctgttc agcccgccgc
     8041 tgtatggcgc gcgtggccgc gtccagcagc gtcccctgcc cggcatcgac gggcaggggc
     8101 ttgtcgtgca ggtccttcag gtccttggcc agggcatcga cgccttccca ttgcgccagc
     8161 acgccagcca gcgccgcgtc atccagcggg tagacctgct gccgccagta gtcgcgcacg
     8221 gcctcggcac gcatcgcgga ttcctgcgcc tgcagctcct catcgaatag gcagccgctg
     8281 tcgaaggcgt gctcgcgcag catgcgctgg caccacgcat cgatggtgtg caccgccgcg
     8341 tcgtccatgg cctcggccgc aacgctgagt tgccaggccg cctgttcgcg ggccggcccg
     8401 gccgggaagt cctgcaacag gctgcgcaag aacgcgtcgt ccgccggcac cggctggcct
     8461 cggaagcagg ccgcggcctc taccaggcgg cgccgaatgc ggtcggagag ctcacgcgtg
     8521 gccgccctcg tgaacgtcat caccaggatc tctgccggca agcgggcctc gcagcggtgc
     8581 ccgagcacga agcgcacata cagtgccgcg atggtccagg tcttgcccgt ccccgcgctg
     8641 gcctcgatga gccggctgcc tcgcagcgga aggtttagcg gatccaaggg ctcactcatc
     8701 gccggctccc tgttccgccg tttcgttgtt caacatcgtc acggtgacgt gatccgccag
     8761 ccattgccga tacggctcgt acagacgctg tgtcgcggcg tcgaagtcaa gatgccccgc
     8821 gaggctggcg aagtccggga agatccgcgc gaggcaggcc tcctggcctt cgccgggcac
     8881 cgggctgcgg aactggcctt cgtaggctgc acgagcgctc tcgggcttag acagccaggc
     8941 cacaccagtc ctcagcgccg tcggcatcgg actgtcaccg cccaggccat cgcggcaggt
     9001 ctgcatcagt gcggtcaagg tcgccatcgc ctcggcctgc gcaggcgggc tcacgcgcac
     9061 catcgcacct tcgccgatga cgatgccatc caccggacag ccacacgcgg ccgaggccag
     9121 gcagcgcacc caagctgtga ggaacttgtc cgcacgcggg atcggaccgg acttcttggt
     9181 gcccgcgtcc gcgactttgc tggcttgcaa ttcgatccag actggcggct cgtgcgcatc
     9241 cgcggcgtgc aggccgacca gccagtcgtc caagacgagg cgatcgtcct gcggatgcac
     9301 cagccgcatc ggctgtttct cgcgcacgaa tggatgttcg gccaggacct gctgccaggc
     9361 ggcggccacc ggcagcagtg tctgcagcag cccatcctga gtgcgacggc cgggcccggc
     9421 catcggcagg cgcccggcgc gttgcaagcg tgccacctgc tgcgccagga aatccggcaa
     9481 cggcggtggc gcttcagctg gagcctcagt ccagccccgg caggcgcgca gcacgtcgtc
     9541 cagaagcgcc caacgttcca gcttgtcacc cccaaaggac tcatcgtcct cgtcgacaac
     9601 gtcgatctcg ctgaacgaca cctgcaggcg gtggcggaag aactccttca ccgggttgcg
     9661 caggaaggcg gccaggccct ggaccgtcaa cggccgcagg gcgcgctcgc cgagggcggc
     9721 gccctcagcc gacgctgccg atccaaccgg caccgcgctc ggcgtgcgat cgtcatgcgc
     9781 cgtccgccac tccccggcat aggtgaacag gcggcctgga tcgccggccg ccgatggttc
     9841 gaagtaccgg cggctgaacg gctgcaaagg gtgttccgtc gtgcgctgcg ccagcacctc
     9901 ggcgccccag ccgctggcca ggtagtcgcg cagctgtgcc accagggtcg atggcggctg
     9961 ctcctggttg tcgcgcgggc tgcggcctgc ccagctgacg gagagcacgc gccgtgccga
    10021 cagcagagca tccaacatca gttgccggtc gtcgtcgcgc cgcgagcggt cgccgggctg
    10081 ggcctggccg ggcaaggcca tgaggtcgaa gtcgctacgg ctgcctcgcc gcgggtagtc
    10141 accgtcgttc atgcccagca ggcacgccac ctggaaagga atggcgcgca acggcagcag
    10201 cgtgcagaag gtgaccccgc cggccttgaa gcgtcgcgac acaccgggct cgtcgacggt
    10261 atccagccag gcttcacgag cgacggccaa cggcatcggc tcgtcgaagg tggcggcctc
    10321 gcacgcctgc aaccactgcg ccagcgcatc gctcagcgcg gccagaattg cgcgctcggc
    10381 gtcgtcggtg gcagcgaaga cgtcagccag caggctgcgc aggcggtctg cccatccctg
    10441 cggcgtgcgc ggcatggatg catcagccca ccaatcggcc agctggtgca gcagatcggc
    10501 cagtgcgcca gccaggcccg cgctcaggcc ggccacttcg gcatagggct cgataccggc
    10561 gaagcccggc gccccggcaa gttcccccgt cgcgtacccc aacagcatgc gccgcaggcc
    10621 gaaggcccag gtgttctgtt caccgccagc gcccaacccc agactgtcgc ggtgggccgc
    10681 gtccaggccc cagcgcacgc cggcaccctc gatccactgc gccagagtgg ggagatcctc
    10741 gggctggacc ccgaaacggc tggcgacggc gggaacgtcg agcaggtcgc ggatctggct
    10801 ggcgcccagg cgctcctgtg gcatccgcag cagccattcg agcgccacca tcaggggctg
    10861 gtggccgcgg tccttctggt cggcgatgcc ccacgggatg tgccgcgcat cccagcgcgg
    10921 atactggccg aacaccgagc ggatggcagg tgcgaaggtg tcgatgtccg gcaccatgac
    10981 gacgatgtcg cgcgggctca aggcctgccc gcccggcggg ttggcgagca gatccagcaa
    11041 ctggtcgtgc aggatctcca cctcgcgttg cgcgctgtga gcgacgtgga agacgatgga
    11101 gcggtcgtcc agccgcgcac cctgacccgt atgctcgccc agcggcagaa ggtcgcgcac
    11161 ggcggcctgc acctgctgca gcagtgtggt gccgggcgcc tcgtcaaaca ggtccacgcg
    11221 gggtagctgg aaccgttggc gggcggcttg gacgtcgtcg aaggcgtcga gctggcgcac
    11281 gaagtcgcgg ctctggcgcc cccacgcggc cagcagtggg tggccgtggg cgtgcatctg
    11341 ctcaaggggc acggcagaca ggtcttggtc gcggcgcagc ggcaggcggc ggcgctcgaa
    11401 gcgcagcagc tcgcggccct cgatgatgtc ggcccagtgg tagcggcagg ggttcggcac
    11461 cgccatcagc acctggcaat gcgcggccag cgcggcgata gcctccaggg tctggtgggg
    11521 gatgtgggtg gtgccgaaca ggacgaggcg gcgcggcagt tccgggaagg ctttggcgcc
    11581 ggagacctgc ggtcgctcca gcgcgtcgag gaagcgccgg tgcgtcccgg cacgcgacag
    11641 atcacgctcg gccggcgtca acgcggcctg cagctcgcgc cacagggcgg gctgccagag
    11701 ctggtccagc ggtacggcat cggccgcacg ggccccgcct tgaccaccct tgcgaatgag
    11761 gtcacggcct tgaccccagt cctcgagcca gtcgccgcga tagacctggt actggtcgaa
    11821 catgtcagcg agccgctgtg ccagttgcag ccgtcgcgac gcgtcgccgt ctgccagaaa
    11881 cccgcgaaca ggctcgaagc ctggctggtc tgcaagcctc ggcagcagct gcatcagccg
    11941 ccacgtcagc ggcagcttgt cgagtggtgc agcccctggc accgcattgc ggccgagcac
    12001 ggcgcggtag gcacgccaga tgaagcgggc cggcaactcg acggtagttg acgcgcagac
    12061 gccgtgggcg ctagccagtt ccatcttgaa ccactcgccc ataccgttgc tctggaccaa
    12121 gatggtttcc tgctcgagcg gtgcgagcgg attcgacgcc agccaggtaa acacggcttc
    12181 ggccaagagt tccaggcgat tgccgtgcag gacgagcagg cctgcttgtt tttcatctcc
    12241 catgcgatgc attctccttg gcaggagagt gacacgctag cagctcaaat cctgagctac
    12301 ttcggtccgt cttgtgcgca ctggcgatcc cacctcgccg tggcggaact gcactcatgc
    12361 gaaacaacta ttgaagacgc cgacatgcag tcagttcggc gacaacggag acgacggcta
    12421 cgccaccaat gacgtgatgg ggcccgagct gtgccagggt gacacatgcc gcatgccgaa
    12481 gtgcgccaag caggtcgggc gagacgctcg tagtccctga gcggctaagt ttctggctga
    12541 cgacttggtc ctggatcgct caaagtccct ggcggccaca gcgaacttcc aggcgtgtcg
    12601 aagccaaggt cgccgaccct caagaccacg tcgtgaagca cgtaactcag catgctccgc
    12661 ggggcatcgg ttgcgtatga gcatctcgac ctagtcgacg cgggcggtct tcgtcatcgt
    12721 gtagcacaga gccgctcatt ggttgcgccg ctccggctct aacggcagat gaacgcgatt
    12781 cagacaaatg acccgcaacc gaggggccct ccaccaagct catcgccaca tattcctcag
    12841 ctgcaaggtc agaagatgaa ctattggcca ggactcgggt ttccccttga acgttggtag
    12901 ctcgttcttt gagcttcatg tgccagatgc tgatgccatg cctcattcag atcacttgac
    12961 ttcaagcatg gcgcggggcg gttatttaag acacttgact gtacgatata agtgttccat
    13021 agtgagccat gacctctcag accgatctgc cggcggcact tcagaccaag gagcgctact
    13081 cgatcggcga gttgtcgaca ttgaccggcg tgactccgcg gacggtgcgc tactacatcg
    13141 ctcagggctt gctggagcga ccacagggcg agaagcgcgg ggcgcactac ctgcgccgcc
    13201 atctcgaaca gctgctgctg gtgcgccgct ggacagaagc gggcttgagc ctcgatcgag
    13261 tgcgagagct gctcgacggt gcaccggagg atccgcctcg tcgcgcggcc gcacctggct
    13321 cagtggaggt ttggagccgc atcaccgtgg ccgacgggct ggaggtgcat ctggagcccg
    13381 gccgggccgg actttcgccc gagcaagtgc gcgccctggt gcgcagcgtc accgggctct
    13441 atcggcaggt gcggtccggc acgcaatcct gagcgtcatg cgcagacgcc tgatatcggt
    13501 caagcacgaa cagggagaga aatgatggaa atcgagtccc gagccttcgc tctatcggtc
    13561 agtgatcccg gagcacccgc cccggtactg actggcgtga cagcctccgg ccgcctggac
    13621 ggcgtgctct tcaatctggc cctgcgacag acttatcgca acgcaagcga tcgaaacctc
    13681 gaagtggtct acacattccc actcccgtgc gaggcagttc tgctcgcctt cgcatccgag
    13741 ctcaatgggg aacgcacgga aggcgtgatc acggccaagc gcgaggcgga gcgtcagtac
    13801 gaggaagctt tgctgaaagg agatgcgcct gtgatgctgg aggcgcttga tggaggtttg
    13861 tacacagcca acctgggcaa tctcaagccg ggggaagaaa tcgtcctcga tgtgcggttt
    13921 gctcaacttc tttctttcga gcaggggcgc ctgcggttag ccattccgac aaccgttgcc
    13981 cctcgctacg gcaacgcgga atctgctggc cttctgccgc agcaggtgcc gacagcctcg
    14041 ctggatgccg agtaccccct ctccctgtcc gtcttggtca gcggttcgct gtctggcgaa
    14101 gacatccagt gcccgacgca caagttcacg cggaccgaag tggaagacgg cgtccggctg
    14161 gatctgcttc ctggtgcaag cctggatcgc gacgtcgcca tcatcgtgac gccgcgcgaa
    14221 acacgccctt ctctagttgt tcaggcgcgc gacactcgct cgtccgcagc gcccgtcgta
    14281 ctgatggctg cgtttcagcc gcctgcaact gcatctcgca agctgatctc gctgaagctc
    14341 ctggtcgact gctcgggttc gatgggtggt gacagcatcg cttcggccaa gttggcactg
    14401 cgaagcgtct tggctcacct gaccgatagc gacagagtca gcctgagccg cttcggaagc
    14461 actgtcgagc acatcctcgc atccacgtct tactcggctt cggcattggc tcacctttcc
    14521 tcgcttgtcg acgcgatcca ggccgacctc ggcgggactg aaatggaggc agcattgagc
    14581 tccgtcttcg aactgggctc tggcaacgaa ggcatcgaca gcgacgtcct tctcatcacc
    14641 gatggagaga tctggcaggc ccgcgagaca gcagctgcag cgaaggcaag cggacaccgg
    14701 gtgtttgcga tcggcgtggg ctcctcgccg gccgaggccg tcctgcgctc gctggccgaa
    14761 tcaacgggag gtgcttgcga gttcgctgcg ccgggcgagt cgctggaagc cgctgcaaga
    14821 cggatgatcg gccgcatccg acaggtgcca tggcaaggcg ttttcgttga ctggggccaa
    14881 accactcttt gggaggttca gccaccgaca tcggtgtttg gtggagacac ggtcattgcc
    14941 atcgcaggga tggcggctgc gcctactgtt tcgcaggcgc ggttgatgtc gaagcccaaa
    15001 gtcggcgaga acgttgaact ggcgcgaggg gaagcgcagg cgccctgccc tggcgatagc
    15061 ctggcgagga tcgccgcggc acgtcgcatc gccggtaccg aaggagagcc gtcgctgtcg
    15121 ctcgccatcg agtaccagat catgagcaag cagaccaact gcatactggt tcaccagcgc
    15181 gccgacaacg atcgggctac cgacgaagcc gaacttcgtc ggatccactc gatgcttgcc
    15241 gctggatggg gagcaacctc gacgcgcctg tcagctccat ctctgatgtg ccaggacgtc
    15301 tccggactga gctctccgat gctctggcgc agcacggccg catttggcag cgcagatgtg
    15361 aagctgagcg tcatcgacag tgatgaccat cacattccgg ccttccttcg caagcaggct
    15421 gaccatcagc gaccggcttc gctgcgaact ttcgcgttgg cagtcgttga acacatggct
    15481 aacggtgggc aggtacaggg tttgccatcc cattgtgcgt accttgatat ccaccctgac
    15541 ctgcacgacg ctatgttgca agtcagaggc ttgggcgtca gcgaaggagt tgccatgctg
    15601 ctcctggcga actgggtcaa tgcccgcgag gacggattga agagtgcgtt cgtcgaggcc
    15661 agccttgccg aacagctccg aaccgtggac gcatcgcttc aggttcggtg ctctgaacta
    15721 tttgagcgat tgctcggtca gtacggactc gatggctgga gctcgtcgcg tacgcatcga
    15781 cttcggaaag cgatggcgcg agtgctgcct tgagtgccga tgaacttcga gcagagtcct
    15841 gtgaatcaac cagtgcccga ttcattgccg gcagcggcgc ggggcagtca caagcatgtg
    15901 ctggactgga cctcacgccc cgacttcctg cgcgagttct cggaactgct ggcgactccc
    15961 gaagttgacg tcgtgggcgc gaggtacatg ccctgcggct acgggaaacc agcggaagca
    16021 cgcttggaaa gcttcggccc ggacgttctc cccggccacc cggcctgggg tcgcgtcgcg
    16081 gattggtggt tgcggcatcc caagggagcg aacaccccga actgggatat cgctgtgaca
    16141 gcaaggatcg aaggccggcc aggtttggtg cttgtcgagg ccaaggccaa cgtgccggaa
    16201 ctctctcgcg ctggcaagcc aacgtcgcca ggcgcctcta aacgtagcca acagaaccac
    16261 gagcagatcg gtcacgccat tgaccaggcc aacacaggct ggagatcagt ggcgccggag
    16321 gcccgtctca ctcgagacac tcactaccag ctcgcgaatc gcttggcatt tgcgtggaag
    16381 ctcgcctcgg acggtgtgcc catcgtgctc ttgtacctgg gattcacagg cgatgacgga
    16441 atcgcagatg ctggcgaacc gttgcgcgat gactctcact ggaggcagct atttcgcgaa
    16501 cacttgcagg agttgtgcgt cgatggttgc ctcgatcgtc gagttctttt cggtgacgtg
    16561 cctgtccggg tgatctgccg tagccgtccg gtcttgtccg tctctccttc gcgccatttt
    16621 cgaaggccct gattaattca ggtcgcgcgc caagacggac tggcgcgcga aaccgctggt
    16681 tcttcattct cttaggacgc taaagtcact tgctatgcct cgtgagtgcg aagctaccgg
    16741 ccccctggga atctatgaac cagaacaagc acctactgga gagcaacgcg gtggacaagt
    16801 caccctttag ctcggcaacc ctgcgctggc ttacgctcgc tctccttctg ctggtgcttt
    16861 ccgtcgcttg gctcaagcca ctcgatgaac gggccagggc cgaaaccgaa gcgggcctca
    16921 agcgcgcact ggtgacctac gctgtcgcca ggacgatcaa cggaatcgtg tccgtggtgc
    16981 aggaatcgac agtgtcactc cagccggggg gtctaggggt cacgttggcg ccagcgcagt
    17041 tcctcgatcc ggtcaacgac cttgtcgaac agttcgcaag cgtgatgctc gcggtgtgtg
    17101 tctcattcgg catccaactc atgcttctca acatcggcgg atccacagcc gtcaatgtgg
    17161 cgcttacgct ggtggtcttc ctatggctgg cggcaaggtg gcgagagtgg acagctccaa
    17221 gacttctttc cgcattggtt gttggacttg tcttcctgcg ctttgctgtg ccgctgttgg
    17281 cgattgcttc cgagaccaca taccgctatg tactggcgga cgagtattcg gcagcgcagc
    17341 agaaggtaga ggccttcgca gaaggtgcgc gcaatacctc cgcaaccgac tctctcagtc
    17401 tcgcgccaga tcaggcaggt gctcaaaccc tctgggaacg gatcaagaag ttacctgatt
    17461 cggtcgacat cgaagcgaag ctgcgccgaa cgcaagagcg cttcgagagt gtcaagtctt
    17521 ggcttgatcg gaccgttgag cacgttgtac gcctgatggc attgttcgcg ctccaaacgg
    17581 tgttcctgcc actactcttc ctatgggcgc ttgtccggtt gtcctccatc gccattggcg
    17641 caaacagagc gagcaggatg gcttcttaac gccgggacat cgggaaaccg tgcgcagccc
    17701 atgccgcagt caagcattcg ccgggttcag tggcagagag ccttcacatg acacaaagct
    17761 caagcagtct cgattggttt gagaagatca ctggcttccg cgagggcact tacgatgaga
    17821 cccgcgcccg cctatcaatc gagaacggat tgttggtgtc accgttcagc cagcgacgat
    17881 gcggcgtggg gcaactcgaa ctcgcgtcat tgaatgatct tcgggtgcat cgcggggcca
    17941 cttcaggcag cgttcgacga actacagtcc ggtgcctcgc ggcggatgtt cgagcactgc
    18001 attccgatcc cgagttctcg ggtgcactct ttcaggtggc atcgcagttc aacctgctgg
    18061 agatggtgtc ccctaacgtc tcgcccgagg atggggtggc acgctatgcc cacgatcgaa
    18121 cccaaggccc ggcgtgcgca atcgcggccg gtgcggccac gatctaccgc aactactttg
    18181 ctcctgtggg tgagggtcgt ggccagactg cagtgcggca gatcgatgcg ctcgccccaa
    18241 tgggcgaagc gttgtcccac caactgaatc gcccggtaca cgccctgtgg accatgcgaa
    18301 atggctatgc gatctgtacg ctcgacgggc tgagatccat aggcgaatac ctgcggagat
    18361 cgactgaacc cacactcgat cacctgcgtg cgcaactcgc gattggcctg cattggcgag
    18421 tcgaggtgac ggactccgag cagccgacaa ggccaaccgt ttctcaagcg ttttgttctg
    18481 cgttgccggt gagatacacg cacatcccaa ctgcggcttg ggaaccgttt gcgcgccttg
    18541 tcttggaggc agcctacgaa gctaccctgc tggctgcctg caagcaacgg aatgagggag
    18601 gctcacgcac agtgctactg acgcgccttg gcggtggcgc cttcgggaat gacgaccggt
    18661 ggattgacga agccatcgat cgcgcactga gacttgtcga gttcgaaggg cttgacgtct
    18721 gtctggtgag cttcggccgg gtgcacgcgg ccttctccgc tatggacacg gcttggaaca
    18781 gtggcccggc gtctcggcac gaatgaggct tgcatgtctg acccggacca gagtcatctc
    18841 gcaaggcaca ccgcttacgt tgtggtcggc cttggacacc gaagatctgt cttgacgtaa
    18901 gcccccgcga aggcagcaac gcctgggcgg gggcatcgac taggacgctc tttcgagaat
    18961 ctccagtgcc ttcgccctcg cctctcgatg accggagaga ttcaactcag gcaactcgag
    19021 acccacctcg ccggacacat aggctggacc gccgaaatcg ctgtcgtaga actgcccgtc
    19081 gttctcaccg ccatcgtgat atgcggccat cgatgatttg gtgaggtcga cgccatgctc
    19141 gagcgccaga tagtgccagg tccacgcccg caaggcatcg ccatgcgcca acgcccgctc
    19201 ggccgcacct cgcatccagc cggtgtcgcc ctgttgtgcc atgcgttctt cagcccaggc
    19261 ctcacccaag tcagcaagcg cctccaacgc cgatcgggat ccctgctctg ccgccttgcg
    19321 cagccacgag gcacgggact gagaggtcga cgcgattcgc gccatctcca atgcatccac
    19381 ttctcccgtg agccgttccg ccagcgcgaa gaactccgga cggttgaaca ccttgccgta
    19441 ctcaagtgcc gccgcgcgta cgccgcccag agctgcctgc ttgagatgct cttcgtactt
    19501 ccggtaccgg ggctccagcc gcaagtactc atccgcccat ccacgctcga cggaagtcag
    19561 cactctgccc ttcagtgact cgtcgtagag atatgggttc ggcttgccgc agcgatacag
    19621 ggctgccaac agaagatgcc gctccggctt cgacgccgct tcctgctcca ggctgctcac
    19681 caagagggaa gaacccaaga ggcgttcgcg tgacggcaac caggacggaa cgctcttcgc
    19741 cgccacctcc gtccaatcct cgggctcatc gtcctcgccg tcttcatcca cgtatcgagg
    19801 tggaggccgg agcgattcct ccaaggcctt ccgaccgaga gcccccagtt ggcgctcatg
    19861 cgcaaagttc aaaagcgctt cggccatcgc acgcgcggcg ttctgctcat actgcagttg
    19921 aaccgccctt ccgatcacct gaggtagtga gtccttcggc gcttcgccaa tgcctgtgtc
    19981 aaccaccagc gattcggagt gaaatgcggc ccaggatcgg tggccaaagg c
//
LOCUS       DAOORV010000063        19968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3303_length_19968_cov_13.519409, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000063 DAOORV010000000
VERSION     DAOORV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3303_length_19968_cov_13.519409"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKV56_09930"
     CDS             complement(<1..571)
                     /locus_tag="PKV56_09930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX68172.1"
                     /translation="MTDLTKAIDAVLDGVTTATPRVPGVSAAVTDRRGTIYEGARGVR
                     NTGTGEAFEPDTVCAIFSTTKAIAGTACLQLVEEGLLDLDAPAKEYVSQLADVMVIEG
                     FGNDGKPKLRPPRRDITTRQLLTHTAGFTYDFFNETYTRLANEQGQPWVVDASWKSIK
                     TPLLFDPGERWEYGSNIDWAGMVVEGITGK"
     gene            complement(773..1807)
                     /locus_tag="PKV56_09935"
     CDS             complement(773..1807)
                     /locus_tag="PKV56_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX68173.1"
                     /translation="MQELLARLKALDPNASLALRIIACFDELVRGGVNIHGLLGAAAS
                     LAGCVAGYAGQPSARTMRVAPSGEILKDRGTATRDCALEADGMTVWLERDGPAHVNDR
                     MILERLALALAVRSGHRAEDPLRRLGDLLDAGVDVSARRAIAAKLGLAPQLKYRVASL
                     PLFATWRQHRELLEDVVHSPFGPLHVCVLRAEVKTIDASPCGIGVATGIDDLHRSFST
                     ALVSLRLCAVPNVPVVVADDYGGLVDLLAQCSIDRPLLDAEELESVMQHPWAAQTLDA
                     LLRSASVRDASRIAGVHHSTMQARLETMRDLLSFDPMDGIGRTRVGLAFLAWRIRHST
                     ALDLPPPGNR"
     gene            complement(1815..2819)
                     /locus_tag="PKV56_09940"
     CDS             complement(1815..2819)
                     /locus_tag="PKV56_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOX68174.1"
                     /translation="MTERHLGVIEGVGRQARVGRPPFDPELRAGLAIVGGMFPPTVTS
                     ELIDFMRVSYAGPPLDTLIGGRSIRCEDRVFEGYEGHALTASVFRHERASGRRPGILF
                     VHSGGMMFGDRFSGLDRMIDFVELLGVVLVSIEYRLAPEHRDPYPREDVYAALEWMAS
                     NAESLGVRLDRLLIAGASAGGGLAAGAALAARDRGGPRLCGQLLDYPMLDDRGITKST
                     RQFDGIGVWDRVSNETGWSALLGDARGGPDVSAYAAPARATRLHDLPPAFLDVGSAEI
                     FRDETVAYADALWNAGGDAELHVWPGGFHAFDIFAPHTALSRGMIATRRAWMSRVLGD
                     "
     gene            complement(3043..3369)
                     /locus_tag="PKV56_09945"
     CDS             complement(3043..3369)
                     /locus_tag="PKV56_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOX68175.1"
                     /translation="MKQAVLRPQALRDQQGEVRYYRKEGGTHLAVKAAKATNEALDQI
                     EQQPRMGSPALGKLLGIPGLRTWRVGTFPLLWCYFERGDHLDVVRLLGERQDIIAILG
                     DEFGSN"
     gene            complement(3366..3617)
                     /locus_tag="PKV56_09950"
     CDS             complement(3366..3617)
                     /locus_tag="PKV56_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOX68176.1"
                     /translation="MSRQTMSFALPESMREYIDHRVAAGNYGNTSEYIRDLVRRDQEE
                     QAKKRLRDLIEEGLASGPGRRRTKADEKELLAIARGEID"
     gene            complement(3915..4256)
                     /locus_tag="PKV56_09955"
     CDS             complement(3915..4256)
                     /locus_tag="PKV56_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68177.1"
                     /translation="MLPGLTPAEELSFEQGLASFLQAHDLLADGTQLQMTIDSASRPL
                     STTDQVDFVAWLMLSMPVASVHIVGTPSPVKQGQVVFRALRSDLALGPVLALYRLGRL
                     RAEHVVDVLGA"
     gene            complement(4348..4725)
                     /locus_tag="PKV56_09960"
     CDS             complement(4348..4725)
                     /locus_tag="PKV56_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68178.1"
                     /translation="MPQDIRHIDETAYPLRLFMGGLHVEVRLRQPIDAPASRRWFNAL
                     HAAALAHHLRMDDAGGVIRLFGAGKPVHRSTGDLVLPAVMPGMGRRLLLWIDGSEAVA
                     RMRFWPSPGTCGYRVVVTGERHG"
     gene            complement(4758..6275)
                     /locus_tag="PKV56_09965"
     CDS             complement(4758..6275)
                     /locus_tag="PKV56_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68179.1"
                     /translation="MTQRLTYGEIQQRVQSHGFELVTTTQQWRERATLHVLVRSPSGY
                     EQLRRVGAFVPGGLQVSCQYDRLGRKGETLAVLAASALTGVTIAREFTPKVLKAQGYR
                     LDGWAKLCGPDGQPVYLALEHQGDHRHNPRAPVHLRRGGAEVSMKEQSRRDEDKARRL
                     ASDPRIVLVPVPDLMLSTDREQGVIEIVAGALESKLGWLKHDAGYLVRKAQLLGRLAV
                     GERVLRIPPSWKADAQQRVEAALAATGDQLRLRFVDADPMARTVTLACQVHGVLEPVN
                     INNVLGAVDGSRLGTRCRHCANAAHGDRRRLSDNEVEAAAKAAGFRPLFEPGAYLNNQ
                     QILGWQCLNDPAHEVRDSLGHLQVRGCPDCREARRAQARQASEFAEVSTALRERGDAL
                     LSTEEAYQDQTSRIRFRCGHCGEEAAQSAAKIKRGQRHGCQRLAASQATRRRREFDKL
                     AAQVRGLGIDLLTTADTYAGNRQPVQYREAGSAQTRTAPAYRLRAWADKAGTQRA"
     gene            complement(6561..6836)
                     /locus_tag="PKV56_09970"
     CDS             complement(6561..6836)
                     /locus_tag="PKV56_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX68180.1"
                     /translation="MAVSPQRAFGERVRELRLAGGLTQDDLAERCGLFRTYLSRIETG
                     RANPTLTMIDALASSLGVPIVALFGVPATPVEAKRPAVRPARGRVSR"
     gene            6945..7697
                     /locus_tag="PKV56_09975"
     CDS             6945..7697
                     /locus_tag="PKV56_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68181.1"
                     /translation="MAVSLAGCATGPINTEDSAISLGKNLVLQSVPLGGMAVQALVSA
                     GDGEARGSYRSKSQLFCQTNAQALLNIRERFAALCRRKGATFDGQFCLSPPWSDNVLF
                     SAKLEYRSLGCHMLHVSEAVTVGSPDYLKFLVNEAGYEKAQVRADKLAERLAATADAH
                     ANAQAEQQAIQAREMARLELELPQLRKRGARVCQMASSNLFMYRGYVEDFTDEKLKIA
                     VAEAFVPNAPGMRPTGFQPHTLWDPPIRWRLC"
     gene            7691..8446
                     /locus_tag="PKV56_09980"
     CDS             7691..8446
                     /locus_tag="PKV56_09980"
                     /inference="COORDINATES: protein motif:HMM:NF036629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HOX68182.1"
                     /translation="MLIRERKRSIGEGSSPPVAVRCTRGVKVLGITGKASGVSGHCGE
                     VGRDDADPGWGQGGAMEAKPEVIYGVIEQDDTGTYIGKLVGFPHIEFHGVSAVEAEGE
                     LRRTALDYKASGVLVLESHFVKLVQIDGSATSDRLAPTPDTGAQGVRHADNRAESCDG
                     SLAGQAPDDLNARLQPLLDAFRPQDHGGEFPSGETGRLSRYKRFGADGPPYEVGRPLR
                     KLSDGDWLVEVTMVETGEKTACRLSRIAEDPDA"
     gene            8485..8730
                     /locus_tag="PKV56_09985"
     CDS             8485..8730
                     /locus_tag="PKV56_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68183.1"
                     /translation="MSKRLKAPPHPDFTFVRGHCGRDAIRIDLPSGGIGIVATTISLE
                     ALSAFAASLRRKIAKPTRLARAGGHAQPTDAGGRASV"
     gene            8793..9077
                     /locus_tag="PKV56_09990"
     CDS             8793..9077
                     /locus_tag="PKV56_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOX68184.1"
                     /translation="MANTVVRLEITGQVQGVGFRWSMAEQARRLGVRGWVRNRRDGSV
                     EAMVAGDSASVDQLIVWARKGPPTAAVEAVNVIRDSTETELGSFEQRPTA"
     gene            9165..9719
                     /locus_tag="PKV56_09995"
     CDS             9165..9719
                     /locus_tag="PKV56_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOX68185.1"
                     /translation="MTTLAAIRKQIAELQKKADAIAKAEARAAAQTVRELIARHNLTA
                     DDVGLSGGPGKPKAKAVKAKAPGVPKYRDPATGKTWTGTGKPPAWIADAKNRSKFLIE
                     SEAASQETATTATRPKARAPKAVRGAKAATVKTAKVTKAPMATKVAARKGAGKKAAAK
                     APVDAASAKSSTVDTASAPAPTGA"
     gene            complement(10129..10677)
                     /locus_tag="PKV56_10000"
     CDS             complement(10129..10677)
                     /locus_tag="PKV56_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX68186.1"
                     /translation="MTTKQEVFISVDVETAGPIPGEYSMLTLGACCVDDPSRTFSCSF
                     KPINDNFVPEALEVTGLSMEKLAKEGLRPEAAMQKFGDWIRSQCTELELPVFVGLNAP
                     FDWSFVNYYFHKFAGSNPFGFTAVDIKALYMGATGSTWQDTRSSAMAGKLNPTLKGDH
                     DALHDALYQAELFRLARQLGRR"
     gene            complement(10713..11606)
                     /locus_tag="PKV56_10005"
     CDS             complement(10713..11606)
                     /locus_tag="PKV56_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68187.1"
                     /translation="MSTSVFESETVLPSKELTARAKTLLGFEDIYSRVSNQLRLLINA
                     DQIEAWSKKHHGGKRLVMVDLLREQYPFAIFYGDVGTGKSAMAETIGNRIAADSKIED
                     CMLYKLSNRVRGTGSVGEMGTLISQAFTEIKNSVGKDRRAILIIDEGDSLAATRTQEH
                     SHHEDKVAVNTLIQAIDELRASKGRIFTILCTNRMSVLDPAVLRRASIVEQFQRPSDE
                     QRRELLEMDLADLGLKPPQVAALVAATRAANDKPSWTYSDIRTRLYPAAVALAFPDNA
                     LTFEHLEQALKVVSPSPALVD"
     gene            complement(11603..12100)
                     /locus_tag="PKV56_10010"
     CDS             complement(11603..12100)
                     /locus_tag="PKV56_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68188.1"
                     /translation="MSGTSSSTKTFTYTNEDVSKVVRRFTTDLQMIADSTKTITQEKA
                     KDYGADMEYLAQRGFLKMVDVTLLSGSTEIKAVQYAVEIGNKDLTSSRPGDVLWPAVK
                     DATLRVIVTYTKDYTAEQKQKHKPNLQINWTPTDESTDHISLQQSGGRDYSSSAFGVT
                     RKDFT"
     gene            complement(12097..13050)
                     /locus_tag="PKV56_10015"
     CDS             complement(12097..13050)
                     /locus_tag="PKV56_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HOX68189.1"
                     /translation="MPLTNTQLLWYDSNVLRLPQDKRTEYHAQVDRLIKELRKHVENR
                     AELTVTKVVKAGSFAKYTILRKTNDDAVDVDVVFYITGKDVNHETFADLSETIYQLLI
                     KIYPTKKVEDFEIQRRAATVTFVGSGLAVDIVPVLQDPERPDYGWQFNIQDGSKNETC
                     APCSIQFVYDRKSEDKHFRTLVRMGKRWRNHMKPDGLKSFHIELIMAHLLERNGTGDS
                     IEKRFRDFLLYIAQSGLQDRIDFPENRKSTAVAFTDPVVIVDPVNNSNNAASRITEEE
                     RVEIAKVAKSSWETAYFASTEDDLKVWKEVFGPRFKVEDDE"
     gene            complement(13110..14636)
                     /locus_tag="PKV56_10020"
     CDS             complement(13110..14636)
                     /locus_tag="PKV56_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HOX68190.1"
                     /translation="MNQQPLKPKYFLLAGSASTSAPAESINRAHEFVREVTKKVLDAG
                     DGFVVYTAAEPVNEANQPLVFDWTILREIEACYPGEATSPRVVIVLAERHRRDSMNAE
                     QRALIAKLSARGLAKIDTIPDAIVTGGNVGDAQAEHAAGMIALGGGKGVSDRAYKLMK
                     RGIPVYPLDLKIGANSEDGDGALGLHRTFMSAPLPFLSHTGAAAVSKTPSLSLEEPVL
                     PIVEVAARVVAILGGELAAEVCAAPTDVLVLTALPVELSAARLAFGINEETPAAKTDI
                     GQNHWRAQVRTTKGTLATCTIATFGTAGNVDAAATTATLLMEFKPKLVIMIGIAAGMR
                     NKTALGDVVIADRVVAYEGAALLAGGQVEARPDTYRPTFSTQQDVSNYLASALSVTAR
                     LKLARAKQGLQFPESSTAGDVSKSVMPKAATIASGEKLLRDPEKFRQLRELHGKVEVA
                     EMEAAGIFAACTQHSVPSLVIRGISDFGDEKKDNSFHELASRAAAIIAADMVAFGLGS
                     "
     gene            complement(15357..18890)
                     /locus_tag="PKV56_10025"
     CDS             complement(15357..18890)
                     /locus_tag="PKV56_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68191.1"
                     /translation="MRLTHLDLIRYGRFTDRRIELPLAERDFHFILGPNEAGKSTTRS
                     AISDLLFGIPARTRLAFLHAMPDLRLGAGLMDSRQPGSDAVLEVQRVKGNKQTLRDRA
                     DKPLPDNALAPFIGNTDGDFFNQMFSLDHGRMVQGGRSILAASDNLGQILFQSAAGIA
                     GLGQVRDALQAEADRLWSPRRAKDRQFYIAADELEAATAALKSTTVRTKAWVDAHDAM
                     AAAEQAFNQLREQVAAVRQQRARLERIRRVLPTLQALQSLRAQRDAMGAVVELPPSAA
                     QTLLEAERAMGIEQVAIDHQAALLAQAKTALAEIPANTALLDAAAEITDLDEQRVQYR
                     AYATDMPRRQAECDAQWAIALRLAAQLGWTGAGEQGLLARLPPQPLRTTLARLARDGG
                     PLRQAADATRRAVRKKQAEFDQTTATLAQLPAAQLPLELQAALQRAKKLGDVATAVQA
                     ATQQVAKSRAALETALAALGRWRAPVADLLAMTPPAADALQALQQDSLQDAAQARALA
                     TRITQLAQQAEQAALDARQYRASHDTVSRDELDAARQQRDRIWQSLKADPTTLPQQSD
                     DFELQLNTADALADRRHDTAQEASELQARLAQEERARMDLRQARDEEVQLQQRTDRRQ
                     AQWATLAEGCGLAGLPFEAAPAWLAARSRALDAAAAAADTQTEAQRLQLAVADAWDGL
                     TRALGQSAGQGATTDTAPVGDMAATAAAASLVDVAALDRLMLEAEARAHAAATVSGQR
                     KALDKQRNDAQDALLALADDARAAELDLERWTTQWRQGLAEAGLAADAEPSAVEAQLA
                     QLDEMAQALEAMRRIRVDRLDKMQADLDRHAAAAQALAQRLQPTLAAQPADLIVRALT
                     AQLAEARALQERAQRLRVEVTGADEQLRAATLRRDQARASLAALMQRAGTASIAALPD
                     AIARSDRWRALQTEATAAERAAAEAGDGLSLAQLQGEASGTDPIQLVGELGSLAAKDE
                     ELVNALSAAAAHRQAATATLQAIAGTADAAQAEARRQQALAAMADAVERYIKVFTAAR
                     LLGWSIERYRETRQGPMLSAASGVFAQLTRGSFDRLVVDFEHDPPRLQGHRPDGALVD
                     IDGMSEGTQDQLFLALRLAALDLHLNHAQALPFIADDLFVNHDDARAIAGLQALGELS
                     RKTQVLFFTHHAHLLPLVQQVFADRVNIVRL"
     gene            complement(18894..>19968)
                     /locus_tag="PKV56_10030"
     CDS             complement(18894..>19968)
                     /locus_tag="PKV56_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOX68192.1"
                     /translation="SWKDFNTGIHFAGQMGRLHAAGIPVYLLLGNHDAESEMTRKLVL
                     PPNVHRFPSTKAASFEIPALRVALHGRSFKDAATTENLAVHYPDPVPGWLNIGVLHTA
                     LEGSSAHAHYAPCSLAELHARGYQYWALGHVHEHQVWRGASTIAFPGNLQGRHIREPG
                     PRGALLLTTEGAEVQSVERLIVDVLRWHALDVDVSACQDLASAVLCTGQAMGDLLQQA
                     GSDHPLALRITLTGATSAHGELFGLEAQLRAEVLAQAATLGLNRLWVEKLVLATTPHD
                     TASDLAARSDALADLQALLAQAPADLALVQGLQADLMGMIGKLPAEVITAVPVLQDIR
                     AGRLDALLQQTAPSLLAHLAKAG"
BASE COUNT         3210 a         6577 c         6659 g         3522 t
ORIGIN      
        1 gcttgccggt gatgccctcc accaccatgc cggcccagtc gatgttgctg ccgtattccc
       61 agcgctcgcc cggatcgaat agcagcggcg tcttgatcga cttccacgat gcatcgacga
      121 cccagggctg gccctgctcg ttggcgaggc gcgtgtaggt ttcattgaag aagtcgtagg
      181 tgaacccggc cgtgtgcgtg agcagctgcc gcgtggtgat gtcgcgccgc ggtgggcgca
      241 gcttcggctt gccgtcattg ccgaaaccct cgatcaccat gacgtcggcc agctgggaca
      301 cgtactcctt cgcgggcgcg tcgaggtcga gaaggccctc ctcgaccagc tgcaggcacg
      361 ccgtcccggc gatggccttg gttgtcgaga agatcgcgca cacggtgtcg ggttcgaagg
      421 cctcgccggt gccggtgttc cggacgccgc gagcgccttc gtagatcgtg ccgcggcggt
      481 cggtcaccgc tgccgacacc ccgggaacgc gtggtgtggc cgtggtgacg ccgtccagca
      541 cagcgtcgat cgcttttgtc agatctgtca atgtcgtctc cggttggtgt tgggtcgtcg
      601 ccgcagaccc agtaccggtt gcaggttgcc tgcgcgtgct tgcgacgatg acctcgcaac
      661 gcggttccgg tgatctgcct gtgacggcga gacggtatcc atccaggcga tcgccagtaa
      721 ccgccgcacg gcgcgcgcag gcaggccact gcccgccagt tggcggttga cgctagcggt
      781 tgccgggggg cgggaggtcg agtgccgtcg agtgccgtat gcgccaggcc aggaaggcca
      841 ggccgacccg ggtgcgaccg atgccgtcca tggggtcgaa cgagagcagg tcgcgcatgg
      901 tctccagccg cgcctgcatc gtgctgtggt ggacgccggc gatgcgcgac gcgtcgcgga
      961 ccgatgccga gcgcagcaat gcgtccagcg tctgcgcggc ccatggatgc tgcatgacgc
     1021 tctcgagttc ctccgcgtcg agaagcggtc gatcgatcga acattgggcg aggagatcga
     1081 ccaggccgcc gtagtcgtcg gcaacgacga ccggcacatt ggggacggcg cacaggcgca
     1141 ggctcacgag cgcggtggag aaggaccggt ggaggtcgtc gatgcccgtc gccacgccga
     1201 ttccgcaggg gctggcatcg atggttttca cctcggcgcg cagcacgcac acatgcagcg
     1261 gaccgaaggg cgagtgcacc acgtcttcca gcagctcgcg gtgctgacgc cacgtcgcga
     1321 agagcggcaa gctggcgacg cggtacttca gctgcggcgc caggccgagc ttcgctgcga
     1381 tcgcgcgacg cgccgacacg tcgacgcctg cgtcgagcag atcgccgagc cgacgcaagg
     1441 ggtcctccgc ccggtgtccg ctgcgcacgg caagggcgag ggccaggcgc tcgaggatca
     1501 tccggtcgtt cacatgggcg gggccatcgc gctccagcca gacggtcatg ccgtctgctt
     1561 cgagcgcgca atcccgcgtc gctgttccgc ggtccttaag gatctcgccg cttggcgcga
     1621 cgcgcatggt gcgggccgat ggctggcccg catagccggc gacacaaccc gcgagcgagg
     1681 ctgcagcgcc caggagtccg tggatgttga cgccgccccg gaccagctcg tcgaagcagg
     1741 cgatgatcct cagcgcgagg ctcgcgttcg gatcgagtgc cttcagtcga gccagcagtt
     1801 cctgcataca gccttcaatc ccccaggacc cgcgacatcc aggcccgtcg cgtcgcgatc
     1861 atcccacgtg acaacgcggt gtgcggcgcg aagatgtcga acgcgtgaaa cccacccggc
     1921 cagacgtgca gttcagcatc gccgccggcg ttccacagcg cgtcggcata ggcgacggtt
     1981 tcgtctcgaa agatctcggc actgcccacg tcgagaaacg cgggcggcag gtcgtggagc
     2041 cgggtcgcac gggccggcgc ggcatacgcc gacacatcag gaccaccgcg cgcatcgccg
     2101 agcagcgcgc tccagccggt ctcgttgctg acgcgatccc agacaccgat gccgtcgaac
     2161 tgacgcgtgg acttggtgat gcctcggtcg tccagcatcg gatagtccag cagctgcccg
     2221 caaaggcgcg gcccgccccg atcgcgcgcc gcgagcgctg cgccggcggc caagccgccc
     2281 cccgcgctcg cgccggcgat cagcaggcgg tcgagacgca cgccgagcga ctccgcgttc
     2341 gacgccatcc attccagcgc cgcgtacacg tcttcgcgcg ggtaggggtc gcgatgctcc
     2401 ggcgcgagcc ggtactcgat cgacacgagg accaccccca gcagttcgac gaagtcgatc
     2461 atgcggtcca gcccgctgaa tcgatcgccg aacatcatgc cgccggagtg cacgaagagg
     2521 atgccggggc ggcggccaga tgcccgttcg tgacggaaaa cggacgccgt gagcgcgtgt
     2581 ccctcgtatc cctcgaagac ccggtcctcg cagcgaatgc ttcgtccacc gatcagggtg
     2641 tcgaggggcg ggcctgcgta ggacacccgc atgaagtcga tcagctcgct ggtcacggtc
     2701 ggcgggaaca tgccgcccac gatcgccaga ccggcgcgca gttccgggtc gaagggcgga
     2761 cggcccaccc gtgcttgccg gcctacgccc tcgatgaccc ctagatggcg ctctgtcatc
     2821 ccccgatctt aaggaacgtg tgctcgtagc gtcgtccaag tgattacgaa cgtcagcttc
     2881 ctggacgcgc aggaaggggc accggtgctc ggtcgtctga cagcttccgg cagcctcgaa
     2941 aggcggcagt gggtcggtcc cggccgacca ttccggcgat tgacctgccg tagaccggtc
     3001 gctggccgcc gacccggcgc tgcgacgatg caagcggcgg agtcagttcg agccgaactc
     3061 gtcccccaga atcgcgatga tgtcttggcg ctcgcccagc agccggacca cgtccagatg
     3121 gtctccacgc tcgaagtagc accacagcag cggaaacgtc cccacccgcc aggtccgaag
     3181 tcccggtatg cccagtagct tgccgagcgc tggagagccc atgcggggct gctgttcgat
     3241 ctggtccagc gcttcgttgg tggccttggc tgccttcacc gccagatggg tgccgccctc
     3301 cttgcggtag taccggacct cgccttgctg gtcgcgcagc gcctgcgggc gcagaacagc
     3361 ctgcttcaat cgatctcgcc gcgagcgatc gccagcagtt ctttttcgtc ggccttggtg
     3421 cgacgccgac cggggccaga tgccagccct tcttcgatga gatcgcgcag ccgcttcttg
     3481 gcctgttcct cttggtcgcg ccggaccaga tcacggatgt actcgctggt gttgccgtag
     3541 ttgccggcag ccactcggtg gtcgatgtac tcgcgcatcg actcgggcaa cgcaaagctc
     3601 atggtttggc ggctcatgcc ggcatcttgg tcggtttgac aacggttgtc aacattgctg
     3661 gctggaagtc ctggtcaaga aatgctgcgg cgccttgtca cgacaccaac cgaactgcct
     3721 gacccgctgc acgctgaatc agcaccgcgt cgaacttcga tgccaaccgc gttgaagaac
     3781 gcatgggggc tgactcccag ccctttgctc ctgcggtgcc caactgcggc cgcctggtcg
     3841 tatgtgtcca aggtcagcgg cgctctgggg gcacactttt cgcagcgtga gccttttccc
     3901 aacttcaatt cacgtcaggc acccaagaca tcgacgacat gttcagcgcg cagccggccc
     3961 agccggtaca gcgccagcac cgggccgagg gccagatcgc tgcgcaaggc gcggaaaacg
     4021 acctgaccct gcttgaccgg gcttggtgtc cccacaatgt gcacgctcgc caccggcatc
     4081 gacagcatca gccaggccac gaaatcgact tggtccgtcg tcgacagagg acgcgatgcg
     4141 ctgtcgatcg tcatctgcag ctgagtgccg tcggccagaa ggtcgtgcgc ctggaggaag
     4201 cttgccagcc cctgctcgaa cgacagttcc tctgctggcg tcaggccggg caacatcctg
     4261 atacggatct caaaagcgcc ctcctctgtc tgccccgctt caggtgcggc tcgcgccgca
     4321 cgggcacggc tgttggggga tgcgcgctca gccatggcgc tccccagtga ccacgacccg
     4381 gtagccacag gtcccggggg acggccagaa gcgcatgcgg gccaccgcct cgctcccgtc
     4441 gatccacagc aacagccggc gtcccatgcc gggcattact gcaggcagga ccaggtcgcc
     4501 cgtggatcgg tgcacgggct ttccggcgcc gaacagccgg atgaccccac ccgcgtcgtc
     4561 catgcgcagg tggtgtgcca atgcggcagc gtggagcgca ttgaaccagc gccgcgatgc
     4621 cggcgcgtcg atgggctggc gcagcctgac ctcgacgtgc aggccgccca tgaacagtcg
     4681 cagcggatag gccgtctcgt cgatgtgccg gatgtcttgc ggcatggtga tgctcctgca
     4741 ggcgtgtggt cggggcatca agcccgttgc gtgccggcct tgtcggccca ggcacgcagg
     4801 cggtaggcgg gcgcggtccg cgtctgcgcc gatcctgcct ctcggtactg gaccggctga
     4861 cggttgcccg cataggtgtc ggccgtggtc agcaggtcga tgcccagtcc cctgacctgc
     4921 gcagcgagct tgtcaaactc gcgccgccgg cgggtcgctt gcgatgcggc caggcgctgg
     4981 catccgtgcc gctggccgcg cttgatcttg gccgcagact gcgctgcctc ctcgccgcag
     5041 tggccgcacc ggaagcggat ccgcgaggtc tggtcctggt acgcctcctc ggtcgacagc
     5101 agcgcgtccc cccgttccct cagcgcggtg gacacctcgg caaactcgct ggcttgccgc
     5161 gcctgcgccc tgcgcgcctc cctgcagtca gggcagcccc gcacctgaag gtggcccagg
     5221 ctgtcccgca cctcgtgcgc cgggtcgttc aggcactgcc agcccaggat ctgctggttg
     5281 ttgaggtacg cgccaggctc gaacagcggc ctgaagccgg cggcctttgc cgcggcctcg
     5341 acctcgttgt ccgacagccg gcgcctgtcg ccatgcgccg cgttggcgca atgccggcac
     5401 cgcgtgccca ggcgactgcc gtcgacggcg ccgaggacgt tgttgatgtt gaccggctcg
     5461 agcacgccat ggacctggca ggccagggtc acggtgcgtg ccattgggtc ggcgtccacg
     5521 aagcgcagcc gcagctggtc gccggtcgct gccaacgcgg cctccacgcg ttgctgcgca
     5581 tccgccttcc aggacggagg aatcctgagc acccgctcgc cgacggccaa ccggcccagg
     5641 agctgggcct tgcggaccag gtagcccgca tcgtgcttga gccagccgag cttggactcc
     5701 agcgcgccgg cgacgatctc gatgaccccc tgctcgcggt cggtggacag catcaggtcc
     5761 gggaccggca ccagtacgat gcgcgggtcg ctggccagcc gccgggcctt gtcctcgtcg
     5821 cgccggctct gctccttcat cgagacctcg gcacccccgc gccggaggtg cacgggggca
     5881 cgcgggttgt gccgatggtc gccctggtgt tcgagcgcga ggtacaccgg ttgtccatcc
     5941 ggtccgcaca gcttggccca tccgtcgagg cgatagccct gggccttcag caccttcggc
     6001 gtgaactcgc gggcgatcgt gacaccggtg agtgcgctgg ccgccagcac agccagcgtc
     6061 tcgcccttgc gcccgaggcg gtcgtactga cagctcacct gcagcccgcc agggacgaag
     6121 gcgccgaccc gcctgagctg ctcgtacccg ctgggtgagc gcacgaggac atggagggtt
     6181 gcgcgttcgc gccactgctg cgtggtcgtg accagctcga agccgtgcga ttgcacgcgt
     6241 tgctgaatct cgccgtaggt caatctctga gtcacgttct tgctccttgt cgttggtaac
     6301 aaggtgactt tgcggacccg gagcgatcga cgaagccggt ttcggtcccg ttggcgtcgt
     6361 gacgcgtcat tcaacaagga cgccaccagc tttcgcgcat cggcatgggt tggtggtgga
     6421 cgcgcgcggc aggccagaca ccccgcgcga cggtcgcgtg aaagtggcgg ccgcgtcgac
     6481 gaccgatgcg tgccagcgtt gttcgcctgc ccgagcggct tctacgtcag acggcgaagc
     6541 cgtgggggtc tgcgagggcc tcagcgagag acgcggccac gtgccggccg caccgccgga
     6601 cgcttggctt cgaccggcgt ggcaggcaca ccgaagagcg ccacgatggg aacaccgagg
     6661 ctggaggcca gggcgtcgat catggtcagg gtcgggttgg cccggcctgt ctcgatgcgg
     6721 ctaaggtacg tgcggaagag tccgcagcgc tcggccagat cgtcctgcgt gagcccgccc
     6781 gccagccgaa gctcgcgcac acgttcaccg aaagctcgct ggggggatac ggccacccgg
     6841 gaactttggt taaagtgcac tcattcatac acacactaat tcatacatca ggggtggcga
     6901 tgaggatgca tcacacgacc ggacgcgcgc tgacggcgtt ggctttggcg gtcagcctgg
     6961 ccggatgcgc aaccggcccc atcaacaccg aagactcggc gatttctctg ggcaagaatc
     7021 tggtcctgca gagcgtgccg ctgggtggca tggccgtcca ggccctggtg agcgctggtg
     7081 atggcgaagc ccggggcagc taccggtcca agtcgcagct gttctgccag acgaacgccc
     7141 aggcgctttt gaacatccga gagaggttcg cagcactttg cagacgcaaa ggagcgacct
     7201 ttgatggcca gttctgcctt agcccgccat ggtctgacaa tgtcctcttc agcgcgaagc
     7261 tggagtaccg gagcctcggc tgccacatgc tgcacgtctc ggaggcggtg acggtcggca
     7321 gcccggacta cttgaaattc ctggtcaacg aagcgggcta cgagaaggcg caggtcaggg
     7381 ccgacaagct tgctgaacga ctcgctgcga cggcagacgc ccacgcaaat gcccaggccg
     7441 agcagcaggc gatccaggca cgcgaaatgg caaggctgga gttggaactg ccccaactgc
     7501 gcaagcgggg agcgcgcgtg tgccagatgg cgtccagcaa cctcttcatg taccgcggct
     7561 acgtggagga cttcaccgac gagaagctca agattgccgt ggccgaagcc ttcgtgccaa
     7621 atgcacccgg gatgcggccg accggcttcc agccgcacac gctgtgggat ccgccgattc
     7681 gctggcgcct ttgctgatcc gcgagcgaaa acgctccatt ggtgaaggaa gctcgcctcc
     7741 tgtggctgtg agatgtaccc ggggcgtgaa agtgctcggc atcacaggaa aagccagcgg
     7801 tgtgagcggt cattgcggtg aagttgggcg agatgatgcc gaccccgggt gggggcaggg
     7861 aggtgccatg gaagcaaagc cggaagtcat ctacggtgtg atcgagcagg acgacaccgg
     7921 aacctacatc ggcaagctgg tcggattccc gcacatcgag ttccacggcg tcagtgccgt
     7981 cgaggccgaa ggcgagttgc gacgcaccgc gctcgactac aaggcatccg gcgtgctggt
     8041 gctggaaagc cacttcgtca aattggttca aatcgacggg tccgcgacga gcgatcggtt
     8101 ggcgccgacg ccggacaccg gggcccaggg cgtacgccat gccgacaatc gcgccgagtc
     8161 ttgcgatggg tcactcgccg gccaagcgcc agacgatctc aacgccagac tgcaacctct
     8221 gctcgatgcc ttccgcccgc aagatcacgg cggggagttc ccatcggggg agacagggcg
     8281 cttgtccagg tacaagcgct tcggcgcgga cgggccgccc tacgaggtgg gcaggccgct
     8341 gcgcaagctc agcgatgggg actggctggt ggaggtgacg atggtcgaga cgggcgagaa
     8401 gaccgcttgc aggttgagtc gcatcgccga agatcctgat gcctgagcat cacggccgac
     8461 cggtgatcaa caggagtgca caggatgtcg aagcgcctga aagccccgcc gcaccctgac
     8521 ttcacattcg tccgcggcca ttgcgggcgc gacgccatcc gcatcgatct gccgtcaggc
     8581 ggcatcggca tcgtcgccac gacgatttcg cttgaggcgc tctcagcctt cgccgcatcg
     8641 ttgcggcgca agatcgccaa gcccacacgc ctggcacggg cggggggtca tgcgcagccg
     8701 accgatgccg gcggaagagc ctcggtgtga gtggcgctgc gtccccagcc ctcgagtcgg
     8761 ctacgcttcg cacgaagcag ccggcaccca tcatggcgaa caccgttgtc cgacttgaga
     8821 tcactggtca ggtccagggc gttggctttc gctggtccat ggccgagcaa gcccgtcggc
     8881 tgggcgttcg cggctgggtc cgcaatcgcc gggacgggtc cgtcgaggcc atggttgctg
     8941 gcgactcggc atcggttgac caactgattg tttgggcacg caagggccca ccaacggcgg
     9001 cggtcgaagc cgtcaacgtc attcgggaca gtactgagac cgagctcggt tcattcgagc
     9061 aaaggccgac cgcctgacgg agaggaaagt ggctcgccgc tgagagcggt tcggcgcgct
     9121 cgcaagcgca tagactctgg ggcgtctcat tcaaggcaga gcccatgacc acactcgctg
     9181 cgatccgcaa gcaaatcgct gaactccaga agaaggccga tgccattgcc aaggccgagg
     9241 cgcgggcagc agcgcagacc gtgcgcgaac tgatcgcaag gcacaacctc acggcggacg
     9301 acgtcggcct gtcgggtgga cctggcaaac ccaaagccaa ggctgtcaag gcgaaggcgc
     9361 cgggtgtccc gaaataccgt gatccggcca ccggcaagac ttggaccggc accggcaagc
     9421 cccccgcctg gattgccgac gccaagaatc gcagcaaatt cctgatcgag tcggaggctg
     9481 caagccaaga gaccgcgacc acagcgacaa ggcccaaggc ccgcgcaccc aaggccgtcc
     9541 gcggcgccaa ggcggcaacg gtgaagacag ccaaggtcac caaggccccg atggcgacca
     9601 aggttgcagc acgcaagggt gcaggcaaga aggctgctgc aaaggctccg gtggacgccg
     9661 catcggcaaa gtcgagcact gtcgacacgg ctagcgcgcc agccccgacc ggcgcctgat
     9721 cgacagcgac acgggttagc gacggccctt ggcagtcgcc cgcgaggtcc tcggcttcgt
     9781 gggcccggtc ccaactgtcc cgggtcagcg cccagcgcgg gcactgcatt catgacgtca
     9841 ctctacgacg atggcgtcac cgccagtcaa gggcagctgt gcagcggttg ctgccggtcg
     9901 cggacggcgc acgaacttcc ggtgagcgcc atcgagctga cgctcgctcc ggaagctggc
     9961 agcgcctcag gcgtcggctt tttcacgcat agctggcgtc agtgccaagg cgtcccggat
    10021 ttgcggcgcg aacgcctgct tctgcgagct gcgacagact gaaatcgacc cgaaagtgtc
    10081 aactaaactc agggcgaatc agtaccagtc gcttatggcc gtggatggtt atcgccgacc
    10141 cagctggcgg gccagccgaa agagctccgc ttggtagagc gcgtcatgca gggcgtcgtg
    10201 atcacccttc aaggtcgggt tcagcttgcc tgccattgca ctggagcgcg tgtcctgcca
    10261 ggtgctgccc gtcgcaccca tgtagagcgc cttgatgtcc acagccgtga agccgaacgg
    10321 attcgagccc gcgaatttgt ggaagtagta gttgacgaac gaccagtcga agggcgcatt
    10381 gagccccacg aaaaccggca attccaactc cgtgcattga gagcggatcc agtcgccgaa
    10441 cttctgcatc gcagcttcag gcctgagccc ttccttggcc agtttctcca tcgagagacc
    10501 agtgacctcc agggcttcgg gaacgaagtt gtcgttgatc ggcttgaacg agcacgagaa
    10561 cgtccggctc gggtcgtcga cgcagcaggc gcccagcgtg agcatgctgt actcaccggg
    10621 gatcgggccg gccgtctcga catcgacgga aatgaaaact tcctgcttcg tggtcatgag
    10681 cctccctcaa ccgagtgctg gtggccggcc ggtcagtcga ccaaggctgg cgacggcgat
    10741 accaccttga gcgcctgctc caggtgctca aaggtcaacg cattgtccgg gaaggccagc
    10801 gcgactgcag cggggtaaag gcgagtgcga atgtcggagt acgtccagga cggtttgtcg
    10861 ttggctgcgc gggtcgccgc taccagggcc gccacttgcg ggggcttgag tcccagatcg
    10921 gccaggtcca tctccaaaag ctcccggcgc tgctcatcgc tcggccgttg gaattgctca
    10981 acgatcgacg cacgacgcag gactgccgga tcaaggactg acatgcggtt ggtgcacagg
    11041 atggtgaaga tccgcccctt gcttgcccgg agctcatcga ttgcctggat gagcgtgttg
    11101 acggccacct tgtcctcgtg atggctgtgt tcttgagtcc gggtcgcggc gagggaatca
    11161 ccctcgtcga tgatcaggat cgcccggcgg tctttgccga cggaattctt gatctcggtg
    11221 aatgcttgtg agatcagcgt ccccatctcg cccacgctgc ccgtaccgcg gacgcggttg
    11281 ctgagcttgt agagcatgca gtcttcaatc ttggaatcag cggcaatgcg attgccgatc
    11341 gtctccgcca ttgccgattt cccggtgccg acgtctccat agaagatcgc gaaggggtac
    11401 tgctcgcgca gcagatcgac cataaccaag cgcttgccac cgtggtgctt cttgctccat
    11461 gcctcgatct ggtccgcatt gatgagcaac cgcaactggt tgctcactcg gctgtagatg
    11521 tcctcaaagc ccagcagcgt cttggcgcgc gcggtcagct ccttgctcgg aaggacggtc
    11581 tcgctctcga aaacggacgt actcaagtga aatcctttcg cgtcacgcca aaggcactgc
    11641 tgctgtagtc gcggccgccg ctttgctgca gggagatgtg gtctgtgctc tcgtccgtgg
    11701 gcgtccagtt gatctggagg ttcggcttgt gtttctgctt ttgctcagcg gtgtagtcct
    11761 tggtataggt gacgatcacc ctcaaggtgg cgtccttcac cgcaggccag aggacgtcgc
    11821 ccgggcggga tgacgtcaga tccttgttgc cgatctcaac ggcgtactgg acggccttga
    11881 tttccgttga gccgctgagc agcgtcacat cgaccatctt caggaagccg cgctgggcca
    11941 ggtactccat gtcggccccg tagtccttgg ccttctcctg cgtgatggtc ttggtgctgt
    12001 cagcaatcat ctgcaggtcg gtggtgaagc gcctgacgac cttactgacg tcctcgttcg
    12061 tgtacgtgaa cgttttagta ctgctgctgg tgccgctcat tcgtcatcct ccaccttgaa
    12121 gcgcgggccg aagacctctt tccagacctt gaggtcatcc tcggtcgatg cgaagtacgc
    12181 ggtctcccat gaggacttgg ccaccttcgc gatctcaacc ctctcctcct cggtgatccg
    12241 tgaggcggcg ttgttgctgt tgttgaccgg gtcgacgatg accaccgggt cagtgaacgc
    12301 cacggccgtc gactttcggt tctcggggaa gtcgatgcgg tcctgcaggc cggattgcgc
    12361 gatgtacagc aggaagtcgc ggaagcgctt ttcgatgctg tcgccggtgc cgttcctctc
    12421 gagcaggtgc gccatgatca actcgatgtg gaacgacttg aggccatcgg gcttcatgtg
    12481 gttcctccac cgcttgccca tccgcactag ggtccggaag tgtttgtctt cggacttgcg
    12541 gtcgtacaca aactgaatcg agcacggcgc gcaggtttcg ttctttgatc cgtcctggat
    12601 gttaaattgc cagccgtagt cagggcgctc ggggtcctgc agcacgggga caatgtccac
    12661 ggctaggccg ctaccgacga aggtcaccgt ggcggcgcgc cgctggatct cgaagtcctc
    12721 gactttcttc gtcgggtaga tcttgataag cagttggtag atggtctcgc ttaggtcggc
    12781 aaaggtttca tggttgacgt ccttgccggt aatgtagaac acgacgtcga catcgaccgc
    12841 gtcatcgttg gtcttgcgca ggatcgtgta cttagcgaac gagccagcct tgaccacctt
    12901 ggtcacggtc agctcggcgc ggttctcgac atgcttgcgc agctctttga tcaggcgatc
    12961 gacctgcgcg tggtactcgg tacgcttgtc ctgcggcagt cgcaggacgt tgctgtcgta
    13021 ccagagcagt tgggtattcg tcaaaggcaa gaggtcctcc tactagcgct ctgcggcgct
    13081 cgcgaccggt tcaaatgatc tgcgggttgc tatgagccca ggccgaacgc gaccatgtcc
    13141 gcggcgatga tggcagccgc ccgcgacgcc agctcgtgaa atgagttgtc cttcttctcg
    13201 tcaccgaagt cgctgatgcc acgaatgacg agcgatggca cgctgtgctg cgtgcatgcc
    13261 gcgaagatgc cggcggcttc catttcagcc acctcgactt ttccgtgaag ctcccgcagc
    13321 tgacggaact tctcagggtc gcgcaagagc ttctcgccgc ttgcgatggt cgccgctttg
    13381 ggcatcacgc tcttggagac gtcgccagcc gttgaagact ccgggaactg caggccctgc
    13441 ttggctctcg ccagcttgag gcgcgcggtg accgatagcg cggaagcgag gtagttcgac
    13501 acgtcctgct gggtgctgaa agtggggcgg tacgtgtcgg gtcgcgcctc gacctggccg
    13561 ccggccagca gcgcagcgcc ctcgtaggcc accacccgat ccgcaatgac gacatcgccc
    13621 aaggcggtct tgttgcgcat gccagctgcg atgccgatca tgatgaccag cttgggcttg
    13681 aactccatca gaagcgttgc cgtggtggct gcggcgtcaa cgttacctgc ggtgccgaac
    13741 gtcgcgatcg tgcaggtcgc gagggttcct ttggtcgtcc gaacctgggc gcgccagtgg
    13801 ttttggccga tgtccgtttt cgccgcgggc gtttcctcgt tgatcccgaa ggccaggcgg
    13861 gcggcactca actccacagg gagggccgtc agcaccagca catcggttgg cgccgcgcac
    13921 acctcggcgg cgagttcgcc tccgaggatc gcgaccacac gagcagcgac ttcaacgatg
    13981 ggcaggacgg gctcttcaag agagagcgaa ggtgtcttgg acaccgcggc cgcgccggtg
    14041 tggctcaaga agggcaatgg cgccgacatg aatgtcctgt gcaagccgag tgcaccgtct
    14101 ccgtcttcgc tgttggcacc aatcttcaga tccaacgggt agaccgggat tccccgcttc
    14161 atcagcttgt acgcgcggtc ggagaccccc ttgccgccac cgagcgcgat cattcccgcc
    14221 gcatgttcgg cttgtgcatc tccgacattt ccgcccgtga cgatcgcgtc cgggatagtg
    14281 tcgatcttgg ccagcccgcg cgctgacagc ttcgcgatca gcgcacgttg ctcggcattc
    14341 atgctgtcgc ggcgatgccg ctccgccagc acgatgacga cgcgtggcga ggtcgcctca
    14401 ccaggatagc aagcctcgat ctcgcgcaag attgtccagt cgaagaccaa cggctggttt
    14461 gcctcattga cgggctcggc ggctgtgtag accacaaagc cgtcaccagc atccaacact
    14521 ttcttggtga cttcgcggac gaactcgtgc gcccgattaa tcgattcagc tggcgcggaa
    14581 gtcgatgcgc ttccagcgag caggaaatac ttaggcttga gaggctgctg attcatcgac
    14641 ttgtgcaaga ccacgagtca acagcaatcg gatgcacagc gtggacatga ggaggcgctc
    14701 atcgcgaaac cacgacgaag gttcccgtag acgcgttgct gacagcctcc gctcactgtc
    14761 accgctaggt ctcgctccta atttttgggg cgtagatggc gtaactagac gtttctgact
    14821 atagcagcgc ccgggcatcg aagttcagtg ggctttgtcg agccctgtgt cgcccaggta
    14881 ttgacggagg ccgctgctct gagtccgacc ttattggccc gaaccgctca tccagtgccc
    14941 gcttccgagc agctaaggca cgaatccgaa ggtccgttct gggtcgacca cggcctgccg
    15001 gcggtcgaag aagcggctgt tcagttcgcg atccccagcg ccgtctcgct catcagcaat
    15061 gttgtggatg agcgaccggt actacctcaa gcggcgggcc gggtccgtgc gcgaaggtca
    15121 gcttgtcggc ggcgccgaca ctcgtccgtg gaactcgacc gtcagcagcc agtcttgagc
    15181 gacctgccaa atcggcaccg ccaagctgtg aatcccggcg cccttgagtt ctcactcgaa
    15241 accccatgcc agtgggtgca gctgagcaag cctaggcgcg ttgtgacacc tatgtggctt
    15301 caaggccgca ccagccgatc ggcgattgcg agctgctgat gatccgctgc cgagcttcag
    15361 agtcggacga tgttcaccct gtctgcgaac acctgctgca ccaacggcag caggtgcgcg
    15421 tggtgggtga agaacaagac ttgggtcttg cgcgacaact cgcccagcgc ctgcaggccg
    15481 gcgatggcac gggcatcgtc gtgattgacg aacaggtcat cagcgatgaa cggcagcgcc
    15541 tgcgcatggt tcaggtgcag gtccagcgca gccaggcgca acgccaggaa cagctggtcc
    15601 tgcgtgcctt cgctcatgcc gtcgatgtcg acgagggcac catccggtcg gtggccctgc
    15661 agccgtggcg gatcgtgctc gaagtcgacc accaggcggt cgaaggaacc gcgcgtcagt
    15721 tgcgcaaaga cgccgctggc cgcactgagc atcggcccct gccgcgtctc ccggtagcgc
    15781 tcgatcgacc agcccagcag ccgcgctgcg gtgaagacct tgatgtagcg ctccaccgca
    15841 tcggccatgg cagccagcgc ctgttggcgc cgcgcttcgg cctgtgccgc atcagccgtg
    15901 ccggcgatgg cttgcaaggt tgccgtcgcg gcttgtcgat gggccgcagc ggcgctgagc
    15961 gcgttgacca gttcttcgtc cttcgccgcc aggctgccca gctcgccgac cagttggatc
    16021 gggtcggtgc cgctggcctc gccctgcaat tgcgccagac tcagtccgtc gcctgcttcg
    16081 gccgctgcac gttcagccgc ggtggcctcg gtctgcagtg cgcgccagcg gtccgaccgg
    16141 gcgatggcgt cgggcaacgc ggcgatggag gccgtgccag cgcgttgcat cagcgcggcc
    16201 aggctggcgc gcgcctggtc acgccgcagc gtggcggcac gcagctgctc gtcggcgccg
    16261 gtgacctcga cgcgcaagcg ctgggcgcgt tcttgcaggg cgcgcgcctc ggccaactgc
    16321 gcggtcaggg cccgcacgat cagatcggcc ggctgggcgg ccagggtcgg ctgcagccgc
    16381 tgggccaagg cctgggcggc ggcggcatgg cggtccaggt cggcctgcat cttgtcgagg
    16441 cggtcgacac ggatccggcg catcgcctcc agcgcctgcg ccatctcgtc gagctgcgcc
    16501 agctgcgctt ccacggcgga cggctcggca tcggctgcca ggccggcctc ggccaagccc
    16561 tggcgccatt gcgttgtcca gcgctccagg tccagctcgg ccgcccgggc gtcatcggcc
    16621 agcgcaagca aggcatcctg ggcgtcgttg cgctgcttgt ccagtgcctt gcgctggcca
    16681 gacacggtgg cagcggcgtg tgccctggcc tcggcttcca gcatcaggcg gtccagcgcg
    16741 gccacatcga ccaaactggc cgcagcggcc gtcgcggcca tgtcgccgac aggcgcggtg
    16801 tcggtcgtgg cgccctgccc cgccgactgg cccagcgcgc gcgtcagccc gtcccatgcg
    16861 tcggccaccg ccagctgcaa gcgttgcgcc tcggtctgcg tgtcggctgc tgctgcggct
    16921 gcgtccaggg cacggctgcg cgccgccagc caggccggcg ctgcctcgaa cggcagtccg
    16981 gccagcccgc agccttcggc cagcgtggcc cactgcgcct gccggcggtc cgtccgctgc
    17041 tgcaactgga cttcctcgtc gcgtgcctgc cgcaggtcca tgcgcgctcg ttcctcttgc
    17101 gccagccggg cctgcagctc gctggcttcc tgcgccgtgt catgccgccg gtcggccagt
    17161 gcgtcggcgg tgttcagctg cagttcaaag tcgtccgatt gctgcggcaa cgttgtcggg
    17221 tccgccttca aggactgcca gatccggtcg cgttgctgcc gggcagcgtc cagttcgtcc
    17281 cggctcacgg tgtcgtggct ggcgcggtac tgccgggcgt ccagcgcggc ctgttcggcc
    17341 tgctgcgcca gctgggtgat gcgcgttgcc agcgcgcgtg cctgcgcggc gtcctgcagg
    17401 ctgtcctgct gcaaggcctg cagtgcgtcg gccgccggtg gcgtcatggc cagcaggtcg
    17461 gccaccggcg cgcgccagcg tcccagcgcg gccagtgcgg tctccagtgc ggcacggctt
    17521 ttggccacct gttgcgtggc ggcctgcacc gcggtggcga catcgcccag cttcttggcc
    17581 cgttgcagtg cagcctgcaa ttccaaaggc aactgcgctg ccggcagctg tgccagcgtc
    17641 gcggtggtct ggtcgaactc tgcctgcttc ttgcgcaccg cgcggcgggt ggcatcggcc
    17701 gcctggcgca gtgggccgcc gtcgcgtgcc agacgggcta gcgtagtgcg cagtggctgt
    17761 ggcggtaggc gcgcgagcag cccctgctcg ccggcgccgg tccagcccag ctgtgccgcc
    17821 agacgcagcg cgatggccca ttgcgcatcg cactcggcct gcctcctggg catgtcggtg
    17881 gcataggcgc ggtattgcac gcgctgctcg tcgaggtcgg taatctcggc ggctgcgtcc
    17941 agcaacgctg tgttggccgg gatctcggcc agggcggtct tggcctgggc cagcagagca
    18001 gcctggtggt cgatggccac ctgttcgatg cccatcgccc gttcggcctc gagcagcgtc
    18061 tgcgcggccg acgggggcag ctctacaacg gcgcccatcg cgtcgcgttg cgcacgcagt
    18121 gactgcaagg cctgcagtgt cggcagcacc ctgcggatgc gttcgagccg ggcccgctgc
    18181 tgccgaaccg ccgccacctg ttcgcgcagt tggttgaagg cctgctcggc ggcggccatc
    18241 gcatcatggg cgtccaccca ggccttggtc cgcaccgtgg tgcttttcag cgcggccgtg
    18301 gccgcctcca gctcgtcggc tgcgatgtag aactgccggt ccttggcccg gcgcggtgac
    18361 cacaggcggt cggcctcggc ctgcagggcg tcgcgcacct ggcccagccc ggcgataccg
    18421 gcggccgact ggaacaggat ctggcccagg ttgtcggacg ccgccaggat gctgcgcccg
    18481 ccctggacca tgcgcccatg gtccaggctg aacatctggt tgaagaagtc gccatcggtg
    18541 ttgccgatga agggcgccag ggcgttgtcg ggcaagggct tgtcggcccg atcacgcagg
    18601 gtctgcttgt tgcccttgac acgctgcacc tccagcacgg cgtcgctgcc cggctgccgg
    18661 ctgtccatca ggccggcgcc caggcgtagg tcgggcatcg catgcaggaa ggccagccgg
    18721 gtgcgcgctg ggatgccgaa gagaaggtcg gaaatggctg atcgcgtggt tgacttgccc
    18781 gcctcgttgg gtccgaggat gaagtggaag tcgcgctcgg ccagcggcag ttcgatgcgc
    18841 cggtcggtga agcggccata gcggatgagg tcgaggtgcg tcaggcgcat cggtcagccc
    18901 gccttggcca gatgagccag cagcgacggt gccgtctgct gcagcagcgc gtccaaccgc
    18961 cctgccctga tgtcctgcag caccggcacg gcggtgatca cctcggccgg cagcttgccg
    19021 atcatcccca tcaggtcggc ctgcaggccc tgcaccaggg ccaggtcggc gggggcctgc
    19081 gccagcagcg cctgcaggtc ggcgagcgca tccgagcggg ccgccagatc gctggccgtg
    19141 tcgtgcggcg tggtggccag caccagcttc tccacccaca gccggttcag gcccagggtg
    19201 gcggcctggg ccagcacctc ggcacgcagc tgggcttcca ggccgaacaa ctcgccatgg
    19261 gccgatgtgg cgccggtcag cgtgatacgc agcgccagcg gatggtcgct gccggcctgc
    19321 tgcagcaagt cgcccatcgc ctggccggtg cacagcacgg ccgaggccag gtcctggcag
    19381 gccgacacgt cgacgtccag tgcgtgccag cgcagcacgt cgacgatcag ccgctccacc
    19441 gactggacct cagccccttc cgtggtgagg agcagcgccc cgcgcggacc gggctcgcgg
    19501 atgtgtcgac cctgcagatt gccgggaaag gcgatggtgc tggcgccgcg ccacacctgg
    19561 tgttcatgca catggcccag cgcccagtat tggtagccgc gggcgtgcag ctcggccagc
    19621 gagcatggcg cgtagtgggc atgggcgctg ctgccttcca gcgcggtgtg cagcacgccg
    19681 atgttcagcc agccgggcac cgggtcgggg tagtgcacgg ccaggttctc ggtggtggcg
    19741 gcgtccttga agctgcggcc gtgcagcgcg acacgcagcg ccgggatctc gaagctggcc
    19801 gccttggtcg aggggaagcg gtgcacgttg ggcggcagca ccagcttgcg ggtcatttcg
    19861 ctttcggcgt cgtggttgcc cagcagcagg tagaccggga tgcctgccgc atgcagccgc
    19921 cccatctggc cggcgaagtg gatgccggtg ttgaagtcct tccagctg
//
LOCUS       DAOORV010000064        19860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3331_length_19860_cov_7.678667, whole genome shotgun sequence.
ACCESSION   DAOORV010000064 DAOORV010000000
VERSION     DAOORV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_19860_cov_7.678667"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2196
                     /locus_tag="PKV56_10035"
     CDS             <1..2196
                     /locus_tag="PKV56_10035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX68193.1"
                     /translation="TCASCVARVEKALLKVPGVASASVNIATERATLEALPSVPVAAL
                     LAAIEKAGYTAREIGEPISQGVPRWPAWWPVALSAAFTLPLVAPMLLQVLGVAWMPSG
                     WLQLMLAAPVQFGLGWRFYRAGWMAVRAGAGNMDLLVALGTSAAFGLSVYLLFEHADH
                     GMPHLYFEASAAVITLVLLGKWLEGRAKRQTTDAIRALNALRPATAKVLRGDAGAEIE
                     VEVPVGQVSVGDRLRVHPGERIAVDGEITEGRSHVDESLITGEGMPVTKGIGDRVTGS
                     SLNAEGVLTLKTLAVGAETTLARIIRMVESAQAAKAPIQRLVDRVSAVFVPVVLAVAL
                     LTLIGWVATTGRWEQAIVNAVAVLVIACPCALGLATPTAIMVGTGVAARQGILIKDAQ
                     ALELAHAVTTVAFDKTGTLTEGRPALVALQPAPGHTREQVLDLSAALQRTSEHPLARA
                     VTAAALAEGLTPPNAHDAHALPGRGIEATVSGQVLALGSSRLLRELGLDAQALTTEAD
                     RLQAQGCTISWLMRRAAGDSAPAELLGLLAFGDAVKPSARAAVARLHELGIHTVMLTG
                     DNQGSAEGVARQLGIESFRAEVLPADKAAAVQALRSGGQIVAMVGDGINDAPALAAAD
                     VGIAMSTGTDVAMETAGITLMRGDPRLVADALDVSRRTYSTIRRGLFWAFAYNVVGIP
                     LAAFGLLNPVIAGAAMALSSVSVVGNALLLRRWRAMPQDSETSVPPRTA"
     gene            complement(2234..2572)
                     /locus_tag="PKV56_10040"
     CDS             complement(2234..2572)
                     /locus_tag="PKV56_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HOX68194.1"
                     /translation="MKNPAPALLAVLCMALAAPPTAQAAGGEGMNVDQGVPAFDPTPG
                     EVRKVDREAGKVTLRHGEIRNLDMPPMTMVFAVRDKAKLDALKAGDKVKFRAVDEGGK
                     LTITEIDVVK"
     gene            complement(2583..3050)
                     /locus_tag="PKV56_10045"
     CDS             complement(2583..3050)
                     /locus_tag="PKV56_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOX68195.1"
                     /translation="MMRRRLVLQAAGAFLLGVPPMTQAGTDGPLVTVWKSPTCGCCKD
                     WISHLQAHGFRVQTHEIGNTDARARLRMPLALGSCHTAEVGGYAIEGHVPAREVLRLL
                     REKPRDVVGLAVPAMPLGSPGMDGPEYGGRRDPFDVLLVRKDGSAIPYQSYNR"
     gene            complement(3047..3538)
                     /locus_tag="PKV56_10050"
     CDS             complement(3047..3538)
                     /locus_tag="PKV56_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HOX68196.1"
                     /translation="MSLIKTALFATMLATTGFSIAHESGGHARHREVAKKAQQEWGIA
                     GDSRTATRTIEVTMFDAMRFSPDHIDLKQGETVRFRVRNDGQVLHEFVIGTRTKNDEH
                     AALMVRFPNMEHDEPYMAHVPPGQTGEIVWTFNRAGEFDFACLIAGHYQAGMIGKISV
                     AAR"
     gene            complement(3582..5006)
                     /locus_tag="PKV56_10055"
     CDS             complement(3582..5006)
                     /locus_tag="PKV56_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HOX68197.1"
                     /translation="MFSRRHFFRHAGAATAAVTAASVSKVAMAALPEPVLQDKPDTMP
                     PLVPNSGRPYNPVVTLNGWTLPWRMNQGVKEFHLVAEPVVREMAPGFKAHLWGYNGQS
                     PGPTIEVVEGDRVRIFVTNKLPEHTTVHWHGQRLPNGMDGVAGLNQPAIQPGKTFVYE
                     FIARRPGTFMYHPHADEMTQMAMGMMGTWITHPKHRHPLIDEVDRDFVFLLNAYDIDP
                     GAYTPKIMTMLDFNLWSWNSRVFPGIDPLVVRQHDKVRLRIGNLTMTNHPIHLHGHEF
                     MVTGTDGGAVPKTARWPEVTTDIGVGQMRQIEFVADEPGDWALHCHKSHHTMNAMGHN
                     VPTMIGVDHQGIAKRITSLIPDYMVMSERGMADMTEMQMPIPDNTAPMMTGDGPVGSV
                     EMGGMFSLLKVRRDQKAGDYSDPGWYRHPAGTVAYEWNGALQPPQRSESQGGQTMPAR
                     NMPEQDIEVQVRKPAMKQGGHAGH"
     gene            complement(5019..6452)
                     /locus_tag="PKV56_10060"
     CDS             complement(5019..6452)
                     /locus_tag="PKV56_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX68198.1"
                     /translation="MNTIAHLHRPGHLAATIAACLVLAGCASLSPDSGFDAVQHTARD
                     RLGHDVSWVRTEAERIAADQRVAELLGTPLSAEAAVQIALLNNRGLQASFDELGIADA
                     DRVQAGLLPNPGFTFGRLRQGAGSEIDRSLTFNLSHLLTMPMARDIAGQRLEQTRGAA
                     IISVLSLASRTRKAYFTALAAEESVRYMLQVQSAAEAGAELARRQAQTGNWNKLQQAR
                     EQGFYADAALNLARAEQVRGEAREQLTRLLGLWGEQTRFRLPERMPELPASASELPDI
                     ERTAMAQRMDVQAARLRTEQVAKSLGLTRATRFVNALELGVMRNSFSEDQTERGFEIS
                     VELPLFDWGTARVARAEAIYMQSVNRAAQTAVDARSEVRGVYANYRIRHDIARHYRDE
                     IVPLKKRISDENLLRYNGMLIGVFELLADARSQITSVNGYIEALRDFWLAQADLDSAQ
                     LGPVAGSGLSQAMSTPSAAPDGAAGSH"
     gene            complement(6463..6813)
                     /locus_tag="PKV56_10065"
     CDS             complement(6463..6813)
                     /locus_tag="PKV56_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68199.1"
                     /translation="MSAISKAAWLPVCALAGLHFITLSAQAQPEPATGPSRVPRAADP
                     AAAVADVPYRSPFAGYRSFSEATVAPWRESNETVRQRGGWRAYAREASGPAAASASAP
                     ATTPASPDASHRMK"
     gene            complement(6944..7318)
                     /locus_tag="PKV56_10070"
     CDS             complement(6944..7318)
                     /locus_tag="PKV56_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68200.1"
                     /translation="MVIRLRAVLMWVLLVALPFQGFAAASMLGCGPSHHPVSVAALQE
                     VAPAASDHDHQHGAPGDHHHADKASKCSVCAACCVSAALPAAALVFAAMATADVPSSL
                     LSMGAVGFLTDGPDRPPRSFLV"
     gene            complement(7360..9138)
                     /gene="sulP"
                     /locus_tag="PKV56_10075"
     CDS             complement(7360..9138)
                     /gene="sulP"
                     /locus_tag="PKV56_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOX68201.1"
                     /translation="MTPGATTRPSGAGRRWLPSLPVLEWGRRYDRETLTRDAVAALIV
                     TIMLIPQSLAYAQLAGLPPEVGLYASVAPLLLYAIFGSSRVLAVGPAAVVSLMTAAAI
                     GNHAVAGSPQYWSAAITLAFLSGAMLVLMGVLRLGFLANFLSHPVISGFISASGLLIA
                     AGQLKTLMGVKAEGHNLVEMLWALGHQLPNTHVLTLVVGVLATAFLFWVRQGLKPLLR
                     RFGLSDKAADVTAKAGPVAVIAITTGLAWALDWQGQGMKIVGAIPQSLPPLTLPSWDL
                     ALWRELALPALLISIVGFVESVSVGQTLAAKRRQRIEPDQELVALGASNLSAAFTGGL
                     PVTGGFSRSVVNFDAGAQTPAAGVFTAAGILLATLLLTPALYYLPQASLAATIVVAVL
                     SLVDLGILKRVWAYSKPDFAAVAATLVATLTLGVELGLVVGVGVSLALYLLRTSRPHI
                     ATVGLVPGTEHFRNVLRHDVLISPRLVSLRVDGSLYFANARSLEDRINDAVAAHPQLA
                     HVVLQCSAINDVDASALESLEAIEQRLREAGIALHLSEVKGPVMDRLQKTEFMERLTG
                     RVFLTHYQAIAQLTPDLLAGNAPDRP"
     gene            complement(9138..10817)
                     /locus_tag="PKV56_10080"
     CDS             complement(9138..10817)
                     /locus_tag="PKV56_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HOX68202.1"
                     /translation="MDIRPLTGELSVAPQIAAADMQAVADAGFRSVICNRPDGEGADQ
                     PTCDELERAAVERGLQLRYLPAESGKVTDEQGAEFGALMAELPKPVLAFCRTGMRSTT
                     MWALSQAGKMPLPQIIELSSKAGFDLKGVVRRIVNGGRTPVEVADATHDVVIIGGGAA
                     GIAVAASLLARAPNLDIAIIDPADIHYYQPGWTMVGAGVFDAATTARTMASVLPRGVH
                     WLKSAVAAFEPERDAVILDGCRVVKYQQLVVCPGLKLDWHGISGLTDTLGRNGVTSNY
                     RYDLAPYTWQLVKQLGQDKRGAKALFTQPPMPIKCAGAPQKAMYLSADHWRRSGVLDD
                     IDIQFCNAGAVLFGVADYVPALMEYVKAYNIGLNFGQTLLAVDGPGRKATFSRANADG
                     SKDIITREFDMIHVVPPQKSPDFIRVSPLSDAAGWVDVDPATLRHKTYPNIFALGDVG
                     NTPNAKTAAAARKQAPVVAHNLLATRGATRGEAKYDGYGSCPLTVERGKIVLAEFTYG
                     GKLAPSFPQWLIDGTRPSRLAWYLKERLLPPLYWEGMLKGREWLAQPEMVG"
     gene            complement(10873..11757)
                     /locus_tag="PKV56_10085"
     CDS             complement(10873..11757)
                     /locus_tag="PKV56_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX68203.1"
                     /translation="MGKDSNLQIEGFFDPGTWTVSYLVLDRPSRQCALVDSVLDYDPK
                     SGRTSTASADRLIARVRELGAQVQWILETHAHADHLSAAPYLKKELGGQLGIGEHITK
                     VQEVFGSLFNAGAEFARDGRQFDHLFADGEAFAIGGLQARAMHTPGHTPACMTYVVID
                     GEDTVAFVGDTLFMPDYGTARCDFPGGDARTLFRSINKVLSLPAATRLYMCHDYQPGG
                     REVQFVSTVGEERERNIHVRNGISEEEFVAMRSARDAALDMPTLILPSVQVNMRAGQW
                     PEPEANGTRYIKIPLNAV"
     gene            complement(11809..12003)
                     /locus_tag="PKV56_10090"
     CDS             complement(11809..12003)
                     /locus_tag="PKV56_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOX68204.1"
                     /translation="MKKNEGTIDRALRVAGGLVLIGLAATGTVGMWGYIGVVPLLTGA
                     IGMCPLYTVLGINTCPVARR"
     gene            12382..13647
                     /locus_tag="PKV56_10095"
     CDS             12382..13647
                     /locus_tag="PKV56_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOX68205.1"
                     /translation="MIVLDPDYNILAANTAYRRQFGTVDKPYLGHKCYRISHHYDVPC
                     DQAGEHCPMRKAFEAKGPDRVLHIHHTPRGPEHVDVELRPILDGDRNVVAYVERLSTV
                     RSASARPSAEGLVGGSPAFNAALSAVQRVAPAMLPVLLLGESGTGKELFARAVHEASE
                     RAHGPFVVVDCSGLTDTLFESEVFGYEKGAFTGAHARKRGLVETAEGGTLFLDEIGDV
                     PLSMQVKLLRLIESGTFRRVGSIETQKADFRLVAATHKPLQQMVADGRFRQDLYYRIS
                     AFPIHLPPLRERADDIPMLVDSFLQRGSSSKHRVSVEARAMARLQAHDWPGNIRELRN
                     VLERARLFADDGIVRAEHLPEGLGCEGPAHAAPERHAPPSERVRAGADDAELRRLATS
                     FTGTRRELATRLGMSERTLYRRLKAMGLA"
     gene            complement(13675..14121)
                     /locus_tag="PKV56_10100"
     CDS             complement(13675..14121)
                     /locus_tag="PKV56_10100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX68206.1"
                     /translation="MTAATQPVRLTQVHDYQFDADFGGERPPIRLDEPAPLGQGTGPS
                     PVQLLASAVGSCLSASLLFSLRKFKQQPDPITCEAQAEVGRSPEGRLRVLSMQVTLTV
                     GVPADALEHLDRVLATFEDYCTVTQSVSQAFPVKVSIFDLHGTRLK"
     gene            complement(14240..16336)
                     /locus_tag="PKV56_10105"
     CDS             complement(14240..16336)
                     /locus_tag="PKV56_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX68207.1"
                     /translation="MKRNSLILLAIGLQPFMATAADKAVLPPVVIRGAVASDASAGRS
                     LSTSDSATLLNSQPGASTYSAGGVSGLPALRGLADDRLKIRIDGAETTSACANHMNAP
                     LSYIEPSQVSRARAVAGISPVSLGGDNIGGVIDVESAAPVFARAGEGLLTQGEIAFLR
                     RSVDDSRTLQLSAGVASETLSLQYSGALAKGESYRDGHGDKVLDTLYKSNNHALTLAA
                     QGEGQRLVFKVGRQSIPYQGYPNQYMDMTDNRGVFANLAYEGSFDWGRLEAKIYGQDT
                     RHEMGFFTREKTGTMPMKTHGRDAGYKLQAELPWQDGKLRIGNEFHRFRLDDWWPPVA
                     GSMMMSPNTYENIVDGRRDRMALFAEWEARLGAKWSTLLGVRHESVRSNAGDVQGYGC
                     GMMCAPDNAAAAAFNARSRKQRDHNIDLTALATYASSQEVEYQLGFARKERSPNLYER
                     YSWGRGMMAMMMVGWFGDGNGYVGDIDLKPEVAHTVSATVNWHDAAKARWEVSLTPYY
                     TYVEDYIDVNVIGTFKPYMRMGASKALLQFANHDAHLYGVNLSWSAVAWDDASWGTGT
                     VKGKLDVTRGRREDGGDLYHQMPVNLSLGLEERWRDLTLSAELQAVARKSAVDDRRDE
                     DRTAGYSLVNLGARYQMNKAFSVQAGVRNLFDRQYDLPLGGANLAAYKASASATQIEP
                     LPGQGRSFDIGMSLRF"
     gene            complement(17070..17879)
                     /locus_tag="PKV56_10110"
     CDS             complement(17070..17879)
                     /locus_tag="PKV56_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOX68208.1"
                     /translation="MKTAPEAVTDDELHAYVDAQLDADRVAAVLAWLQSHPDDAARVA
                     QWQLQRAQLRQLARSVDVGDTPPALLDGVMRAATHARRLARWQQGAAAAVLLAVGLMA
                     GMAIERSVEGGRPPGSGSALAAAPLFVREAAAAHAVFVPEKRHPVEVTAADEAHLVQW
                     LSRRLGAPLKAPSLGAHGYHLLGGRLLPGEGTPRAQFMYENQQGSRVTLFVAAFTADA
                     APSETAFRLVRDGTRTSFYWVEERYGYALIADGSEAEVRGLVSEVYAQLNR"
     gene            complement(17876..18388)
                     /locus_tag="PKV56_10115"
     CDS             complement(17876..18388)
                     /locus_tag="PKV56_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX68209.1"
                     /translation="MGIEQTLGHIPALRRYARLLTGDAARADDLVQDTLERACVKWSL
                     WEPGSVLRSWLLSLMHNLHLNHRRDWRHDDGHAALDDVPEPAHEPLARLGERLDLQRA
                     LAALPPSMREVLLLVTVEEYTYAEAACILDVPVGTVMSRLHRARERLRELMSDPGETP
                     RAHPLQLVKP"
     gene            complement(18413..18796)
                     /locus_tag="PKV56_10120"
     CDS             complement(18413..18796)
                     /locus_tag="PKV56_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68210.1"
                     /translation="MTRPAFLSALVFCAALASGSAVMAQAAPAQEADGVLVGANGMTL
                     YTFDRDAAGSGKSVCNGPCAAKWPPLAAASDAVARGDWSVVIRDDGSRQWAYKGKPVY
                     FWAADQKAGDRTGDGVNNVWHLARP"
     gene            19072..19248
                     /locus_tag="PKV56_10125"
     CDS             19072..19248
                     /locus_tag="PKV56_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68211.1"
                     /translation="MTPDERRLVRHLVTAVLVKLAVLVGLWWGFVRDQRVETDTARTA
                     AHLGATTPDPGATP"
     gene            19245..>19860
                     /locus_tag="PKV56_10130"
     CDS             19245..>19860
                     /locus_tag="PKV56_10130"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004540940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOX68212.1"
                     /translation="MISEQLVDLSRLQFAATAMYHFLFVPLTLGMVWLLVIMESVYVM
                     TGKVVWKDMTRFWGKLFGINFALGVTTGITLEFQFGTNWAYYSHYVGDIFGAPLAIEG
                     LMAFFLESTFIGLFFFGWDRLTRTQHLMVTTLMAVGTNLSALWILIANGWMQNPVGAE
                     FSYQTMRMEMVDFWAIVFNPDAQAKFVHTVSAGYVTGAMFVLSVS"
BASE COUNT         3209 a         6717 c         6686 g         3248 t
ORIGIN      
        1 acatgcgcct cgtgtgtcgc gcgcgtcgag aaggccctgc tgaaggtgcc cggggtcgca
       61 tcggcgtcgg tcaacatcgc gaccgaacgc gccacgctcg aagcgctgcc gtccgtgccg
      121 gtggcggcgt tgctggcggc catcgagaag gccggctaca ccgctcgcga gatcggcgaa
      181 ccgatcagcc agggcgtccc gcgatggccc gcctggtggc cggtggcgct gagtgccgca
      241 ttcacgctgc cgctggtcgc cccgatgctg ctgcaggtcc tgggcgtggc gtggatgccg
      301 tcgggctggc ttcagctgat gctggcggca ccggtgcagt tcgggctggg ctggcgcttc
      361 taccgcgcag gctggatggc cgtgcgggcc ggcgccggca acatggacct gctggtcgcg
      421 ctgggcacgt cggccgcctt cggcctgtcg gtctacctgc tcttcgagca cgcggaccac
      481 ggcatgccgc acctgtactt cgaggcgtct gcggcagtca tcacgctggt gctgctcggc
      541 aagtggctgg agggccgcgc gaagcgccag acgaccgacg cgatccgggc cctgaatgcg
      601 ctgcgaccgg ccacggcgaa ggtgctgcgc ggcgatgccg gtgcggaaat cgaggtcgag
      661 gtgcccgtcg ggcaggtttc ggtcggagac cggctgcggg tgcaccccgg cgagcggatc
      721 gcggtggacg gtgaaatcac cgaaggccgc agccatgtcg acgaatcact gatcaccggc
      781 gaaggcatgc ccgtgaccaa gggcatcggt gaccgggtca ccggcagttc gctcaacgcc
      841 gaaggcgtgc tgaccctgaa gacgctggcg gtcggagccg agaccacgct ggccaggatc
      901 atccgcatgg tcgagtcggc ccaggccgcc aaggcaccga tccagcggct ggtcgatcgc
      961 gtcagcgcgg tgttcgtccc ggtggtgctg gcggtggcac tgctgacgct gatcggctgg
     1021 gtggcgacaa cgggccgctg ggaacaggcg atcgtcaacg cggtggccgt gctggtcatc
     1081 gcgtgcccgt gcgccctggg cctggccacg cccaccgcga tcatggtcgg caccggtgtc
     1141 gcggcacggc agggcatcct gatcaaggac gcgcaggccc tggagctggc gcatgcggtg
     1201 accacggtcg ccttcgacaa gaccggcacg ctgaccgagg gacgccccgc gctggtcgcg
     1261 ttgcagccgg cccccggcca cacgcgagag caggtgctcg atctgtcggc ggcgttgcag
     1321 cgcaccagcg agcatccgct tgcccgggcg gtgacggccg ctgcgcttgc cgaaggactg
     1381 accccgccga acgcacacga cgcgcacgcc ctgccgggtc gcggcatcga agccacggtg
     1441 agcggacagg tgctggcgct gggcagcagc cggctgctgc gggagctggg cctcgatgcg
     1501 caggcactga cgaccgaagc cgatcgactg caggcccagg gctgcacgat ttcctggttg
     1561 atgcgcaggg cagccgggga cagcgccccc gccgagctgc tgggcctgct tgcgttcggc
     1621 gatgcagtca agccatcggc gcgcgctgcg gtggcacgcc tgcatgagct cggcatccac
     1681 accgtgatgc tcaccggcga caaccagggc agcgccgaag gggtggcccg gcagctcggc
     1741 atcgagtcgt ttcgcgccga ggtcctgccg gccgacaagg ccgcggcggt ccaggcgctg
     1801 cggtccggcg gccagatcgt cgcgatggtg ggcgacggca tcaacgacgc cccggcgctc
     1861 gccgctgccg atgtcggcat cgcgatgtcg accggaaccg acgtggcgat ggaaaccgcc
     1921 ggcatcacgc tgatgcgcgg cgacccgcgc ctggtcgccg acgcgctcga tgtctcgcgg
     1981 cgcacctact cgacgatacg gcgcggcctg ttctgggcct tcgcctacaa cgtcgtcggc
     2041 atcccgctgg cggccttcgg cctgctgaat ccggtgatcg ccggcgccgc gatggcactc
     2101 agcagtgtca gcgtcgtcgg caacgcgctg ctgctgcggc gctggcgagc gatgccgcag
     2161 gattccgaaa ccagcgtgcc gccccggacg gcctgaagag cacccgcggc cgaagcgcct
     2221 gctcggcgcg ggctcacttc acgacatcga tctccgtgat ggtgagcttg ccgccttcgt
     2281 caaccgccct gaacttcacc ttgtcgcccg ccttcagcgc gtcgagcttc gccttgtccc
     2341 gaacggcgaa gaccatcgtc atcggcggca tgtccaggtt gcggatctcc ccgtgcctca
     2401 atgtgacctt gccggcctcc ctgtcgacct tgcgcacctc gccaggagtc gggtcgaaag
     2461 caggcacgcc ctgatcaacg ttcatgcctt caccgccggc ggcttgcgcg gtcggcgggg
     2521 cggcgagcgc catgcaaagc acggcaagca atgcgggggc tgggttcttc atggtcggcg
     2581 attcaccggt tgtaggactg ataggggatc gcactgccgt ccttcctgac gagcagcacg
     2641 tcgaacggat cccggcggcc gccgtactcc ggtccgtcca tcccgggtga gccgagcggc
     2701 atcgccggaa cggccagccc gacgacgtcg cgcggcttct ccctgagcag gcgcaggact
     2761 tccctcgcgg gcacgtggcc ttcgatcgcg tagccgccca cttcggcggt atggcacgag
     2821 cccagcgcca gcggcatgcg cagtcgcgcc cgggcgtcgg tgttgccgat ctcgtgggtc
     2881 tgaacgcgga agccgtgggc ctgcaggtgg gagatccagt ccttgcaaca gccgcaggtc
     2941 gggctcttcc acaccgtcac cagcggtccg tcggtgccgg cttgcgtcat cggtggcaca
     3001 cccagcagga aggcgcctgc tgcctgcaaa accagccgac ggcgcatcat cgcgcggcca
     3061 ccgagatctt gccgatcatc ccggcctggt agtgaccggc gatcaggcag gcgaagtcga
     3121 actcgcctgc ccggttgaag gtccagacga tctcgccggt ctggcccggc gggacatggg
     3181 ccatgtaggg ctcgtcgtgt tccatgttcg ggaacctgac catcaaggcg gcgtgctcgt
     3241 cgttcttcgt ccgggtgccg atgacgaatt cgtgcagcac ctgtccgtca ttgcgaaccc
     3301 ggaaccgcac ggtctcgccc tgcttcaggt cgatgtgatc gggcgagaac cgcatcgcgt
     3361 cgaacatcgt cacctcgatc gttcgcgtgg cggtcctgga atcgccggcg attccccact
     3421 cctgctgcgc tttcttggcg acctcgcgat gccttgcatg cccgccactt tcgtgcgcga
     3481 tcgagaagcc tgtagtcgcg agcatcgttg cgaacagcgc ggtcttgatg agtgacatgg
     3541 gtgtcctttt cgtttcgaga gggattcgag acaggcggcg gtcagtgacc ggcgtggccg
     3601 ccctgcttca tggcgggctt gcggacctgc acttcgatgt cctgctccgg catgttcctg
     3661 gcgggcatcg tttgcccacc ctgggactcg gagcgttgcg gaggctgcag cgcgccgttc
     3721 cattcgtagg ccaccgtgcc tgccgggtgc cgataccagc cggggtcgct gtagtcgcca
     3781 gccttctggt cgcgacgcac cttcagcagg ctgaacatgc cgcccatctc gaccgagccc
     3841 accgggccgt cgccggtcat catcggcgcg gtgttgtcgg gaatcggcat ctgcatctcg
     3901 gtcatgtcgg ccatgccgcg ctcgctcatc accatgtagt cggggatcag actggtgatg
     3961 cgcttcgcga tgccctggtg atcgacgccg atcatcgtcg gcacgttgtg ccccatcgcg
     4021 ttcatcgtgt ggtggctctt gtggcaatgc agggcccagt cgccgggctc gtcggcgacg
     4081 aactcgatct gcctcatctg gccgaccccg atgtcggtcg tcacctcggg ccagcgggcg
     4141 gtcttcggca ccgcgccccc gtcggtgccg gtcaccatga actcgtggcc gtgcaggtgg
     4201 atcgggtggt tggtcatcgt caggttgccg atacgcaggc gcaccttgtc gtgctggcgc
     4261 acgaccagcg gatcgatgcc cggaaagacg cggctgttcc agctccacag gttgaagtcg
     4321 agcatcgtca tgatcttcgg cgtgtaggca cccgggtcga tgtcgtaggc gttgagcagg
     4381 aacacgaagt cgcggtcgac ctcgtcgatc agcggatgcc tgtgcttcgg gtgcgtgatc
     4441 caggtgccca tcattcccat cgccatctgc gtcatctcgt cggcgtgcgg gtggtacatg
     4501 aaggtgccgg gacgacgcgc gatgaactcg tagacgaagg tcttgcccgg ctggatcgcc
     4561 ggctggttga gccccgcgac gccgtccatg ccgttgggca ggcgctggcc gtgccagtgc
     4621 acggtggtgt gttcgggcag cttgttggtg acgaagatgc gcacgcggtc gccttccacc
     4681 acctcgatcg tcgggcccgg cgactgcccg ttgtagcccc acaggtgcgc cttgaagccg
     4741 ggcgccatct cgcgcaccac cggctcggcg acgaggtgga attccttgac cccctggttc
     4801 atccgccagg gcaaggtcca gccgttgagc gtgacgacgg ggttgtaggg gcgtccgctg
     4861 ttcggcacca gtggcggcat cgtgtcgggc ttgtcctgca gcacgggctc gggcagggcc
     4921 gccatcgcga ccttgctgac cgaagccgcc gtgacagcgg ccgtcgctgc tccggcatgg
     4981 cgaaagaagt ggcgtcgtga aaacatggtg gcctcgattc agtggctgcc cgccgcgccg
     5041 tcgggggcgg cagacggggt agacatggcc tgcgacaggc ccgacccggc aaccgggccg
     5101 agctgcgcac tgtcgagatc ggcctgcgcc agccagaagt cgcgcagcgc ctcgatgtag
     5161 ccgttgacgc tggtgatctg cgagcgggca tcggcaagca gctcgaacac gccgatcagc
     5221 atgccgttgt agcgaagcag gttctcgtcg gagatgcgct tcttcagcgg cacgatctcg
     5281 tcgcggtagt ggcgggcgat gtcatggcga atgcggtagt tcgcatatac gccgcgcacc
     5341 tcgctgcgcg catcgacggc cgtctgcgcc gcacggttca ccgactgcat gtagatcgcc
     5401 tcggcgcgcg cgacacgcgc ggtgccccag tcgaacaagg gcagctcgac gctgatctcg
     5461 aagccgcgtt cggtctgatc ctcgctgaag gaattgcgca tcacgccgag ctccagcgcg
     5521 ttgacgaagc gcgtcgcgcg tgtcagcccc aggctcttcg ccacctgctc ggtgcgcagc
     5581 cgggcggcct gcacatccat gcgctgcgcc atcgccgtgc gttcgatgtc gggcaactcg
     5641 ctcgccgatg caggcagttc gggcatccgt tcggggagac ggaagcgggt ctgctccccc
     5701 cacagaccga gcagccgcgt gagttgctcg cgtgcctcgc cgcgcacctg ctcggcacgc
     5761 gccaggttca gcgccgcatc ggcatagaag ccctgctctc gcgcctgctg cagcttgttc
     5821 cagttgccgg tctgtgcctg acggcgtgcc agctcggcac ccgcttcggc agcagactgc
     5881 acctgcagca tgtagcgcac ggattcctcg gcagcgagcg cggtgaagta ggccttgcgc
     5941 gtccgtgacg ccagcgacag cacgctgatg atcgcggcgc cgcgcgtctg ctcgaggcgc
     6001 tgcccggcga tatcgcgtgc catcggcatc gtcagcaggt gcgagagatt gaaggtcagg
     6061 gagcgatcga tctcgctgcc ggcgccctgc cgcaaccgcc cgaaggtgaa ccccggattg
     6121 ggcaggaggc cggcctgcac tcggtcggcg tcggcgatgc cgagttcgtc gaacgacgcc
     6181 tgcaggcccc ggttgttcag cagggcgatc tggaccgcgg cctcggccga cagcggcgtc
     6241 ccgagcaact cggcaacccg ctgatcggcg gcgatgcgct cggcttccgt gcgcacccag
     6301 gacacgtcat ggcccagccg gtcccgtgcg gtgtgctgga cggcatcgaa gcctgaatcg
     6361 gggctcaacg aggcgcagcc cgccagaacc agacaggccg cgatcgttgc ggcaaggtgc
     6421 cccggtcgat ggaggtgagc gatggtgttc atgcgggtcg cctcatttca tccggtgact
     6481 ggcatccggc gatgccgggg tcgttgcagg agcggaagcg gatgcagccg cgggcccgct
     6541 cgcctcgcgc gcgtaggcgc gccagccgcc gcgctgccgc accgtctcgt tgctttcgcg
     6601 ccagggcgcc acggttgctt cggagaacga ccgatagccc gcgaagggcg atcgatacgg
     6661 cacgtcggcc actgcagccg caggatcggc cgcccgtggc acgcgggacg ggcctgtcgc
     6721 aggctcaggc tgagcctgtg cggacagcgt gatgaagtgc agccccgcga gggcgcacac
     6781 cggcagccag gccgccttgg aaatcgcaga catgaggaac cccgtccagc gtgccggagg
     6841 cttgccgcct ccaggcacag tcagtgaagc gcacgcgcgg cggtgtcggt gactccgctt
     6901 tcccgccgcg aacatgcacg gcagaaggcg cgtgcggcgc ccgtcagacg aggaaggatc
     6961 gagggggtcg gtccgggccg tcggtcagga agccgacagc gcccatcgac agcagggagc
     7021 tgggcacatc cgccgtcgcc atggcggcga aaaccagcgc ggccgccgga agcgccgcgc
     7081 tcacgcagca ggctgcgcag acgctgcact tggacgcctt gtcggcgtgg tgatggtcac
     7141 ccggagcgcc atgctggtgg tcatggtccg atgcggcagg agccacctcc tgcaaggccg
     7201 cgaccgacac cggatggtgg ctcggaccgc aaccgagcat gctcgccgcg gcaaatccct
     7261 ggaagggaag cgcgaccagc agcacccaca tcagcacagc gcgaagccgg atcaccatgc
     7321 gtcgattgta aaaagaatgc cgcaccggaa atcgctcgac tacggccgat caggggcatt
     7381 gcctgccagc aggtcggggg tcagctgggc aatggcctgg tagtgggtca ggaacacgcg
     7441 cccggtcagg cgctccatga actccgtctt ctggagtcgg tccatcaccg gccccttgac
     7501 ttcggacagg tgcagcgcga tgccggcctc gcgcagccgc tgttcgatgg cttccaggct
     7561 ttcaagggcg ctggcgtcca cgtcgttgat ggccgagcac tgcagcacga catgcgccag
     7621 ctgcgggtgg gcagccacgg catcgttgat gcggtcttcc aggctgcgcg cattggcaaa
     7681 gtagaggctg ccatcgacgc gcaggctcac cagcctgggg ctgatcagca cgtcgtgacg
     7741 cagcacgttg cggaagtgct cggtgcccgg aaccaggccg acggtcgcga tgtgcggccg
     7801 gctcgtgcgc aagaggtaca gcgccagcga cacgccgacc ccgaccacca acccgagctc
     7861 gacgccgagc gtcaacgtgg cgaccagcgt cgcggcgacc gcagcgaaat caggcttcga
     7921 atacgcccac acgcgcttca ggatgccgag gtcgacgagc gacagcaccg cgacgacgat
     7981 ggtcgcagcc agcgaggcct gcggcaggta gtacagcgcg ggcgtcagca gcagcgtggc
     8041 gagcaggatg cccgccgcgg tgaacacgcc ggcagccggc gtctgcgcgc cggcgtcgaa
     8101 attgaccacc gaacgcgaga agccgccggt gaccggcaag cccccggtga atgcagccga
     8161 caggttgctg gcgccgaggg ccaccagttc ctgatcgggt tcgatgcgct gccggcgctt
     8221 ggccgccagc gtctgtccga ccgaaaccga ctcgacgaag ccgacgatgc tgatgagcag
     8281 cgccggcagg gcgagttcgc gccaaagcgc gagatcccag gacggcagcg tcagcggcgg
     8341 caggctctgc ggaatggccc cgacgatctt catgccctgg ccctgccaat cgagcgccca
     8401 ggccagtccg gtggtgatgg cgatcaccgc gaccggtccg gccttggccg tcacatcggc
     8461 agccttgtcg gacagaccga agcgccgcag caagggcttc aggccctggc gcacccagaa
     8521 gaggaaagcg gtcgccagca cgccgacgac gagcgtcagc acatgggtgt tcggcagctg
     8581 atgccccaga gcccacagca tttcgacgag gttgtggcct tcggccttca cacccatcag
     8641 cgtcttgagc tggccggctg caatcagcag gcccgaggcc gagatgaagc ccgagatgac
     8701 gggatggctc aggaagttgg ccaggaagcc cagcctcagc acgcccatca gcaccagcat
     8761 cgcacccgac aggaaagcaa gcgtgatcgc tgcgctccag tactgcggcg aacccgccac
     8821 cgcgtggttg ccgatcgccg ccgccgtcat cagggagacg accgccgcgg ggcccaccgc
     8881 cagaacccgg ctgctgccga agatcgcgta cagcaacagc ggcgccacgc tggcatagag
     8941 cccgacctcg ggcggcaatc cggcgagctg cgcataggcc aggctctgcg gtatcagcat
     9001 gatcgtgacg atcagggctg ccaccgcgtc gcgggtcagg gtctcgcggt catagcgtcg
     9061 cccccattcg agcaccggca ggctcggcag ccagcgcctg ccggcccccg acggccgggt
     9121 cgtcgctcca ggtgtcatca gcccaccatc tcgggctggg ccagccactc gcggcccttc
     9181 agcatgcctt cccagtacag cggcggaagg aggcgctcct tcaggtacca ggccagacgc
     9241 gagggacggg tgccatcgat cagccactgc gggaaactgg gcgccagctt gccgccatag
     9301 gtgaactcgg ccagcacgat cttgccgcgt tccaccgtca gcgggcagga gccgtagccg
     9361 tcgtacttcg cttcaccccg ggtcgctccg cgggtggcga gcaggttgtg cgccaccacg
     9421 ggagcctgct tgcgcgccgc ggcagcagtc ttggcgttgg gggtgttgcc cacgtcgccc
     9481 agggcaaaga tgttggggta ggtcttgtgg cgtagcgtgg ccggatcgac atcgacccag
     9541 cctgccgcat ccgacagcgg gctcacccgg atgaagtcgg gcgatttctg aggcgggacc
     9601 acgtggatca tgtcgaattc tcgggtgatg atgtccttgc tgccgtcggc gttcgccctg
     9661 ctgaacgtgg ccttcctgcc cgggccgtcg acggccagca gagtctgacc gaagttcaat
     9721 ccgatgttgt aggccttcac gtactccatc agtgcgggca cgtaatcggc cacgccgaac
     9781 aggaccgcgc ccgcgttgca gaactggatg tcgatgtcat ccagcacgcc cgagcggcgc
     9841 cagtgatcgg ccgacaggta catcgccttc tggggcgcac cggcgcactt gatcggcatc
     9901 ggcggctgcg tgaacagcgc cttggcaccg cgcttgtcct gtccgagctg cttcaccagt
     9961 tgccaggtgt agggcgcgag gtcgtagcgg tagttggagg tgacgccgtt tcgtccaagc
    10021 gtgtccgtca gcccagagat gccgtgccag tcgagcttca ggcccggaca caccaccagc
    10081 tgctggtact tgacgacacg acacccgtcg aggatgaccg catcgcgctc gggttcgaac
    10141 gccgccaccg ccgacttgag ccagtgcacg cctcgcggca ggaccgaggc catcgtccgg
    10201 gcggtcgtgg ccgcgtcgaa cacgcctgcg cccaccatcg tccagccggg ctggtagtag
    10261 tggatgtcgg ccgggtcgat gatcgcgata tcgaggttcg gggcgcgcgc cagcaggctg
    10321 gccgcaacgg cgatgcccgc tgcaccgcca ccgatgatca ccacatcgtg tgtcgcatcg
    10381 gccacctcca ccggagtccg gcctccgttg acgatgcgcc gcaccacgcc cttcaggtcg
    10441 aagccggctt tcgatgacag ctcgatgatc tgtggcagcg gcatctttcc ggcctgcgag
    10501 agcgcccaca tcgtggtcga tcgcatgccg gtgcgacaga aagccagcac cggtttcggc
    10561 agctcggcca tcagcgcgcc gaactcggca ccctgttcgt cggtgacctt gccagattca
    10621 gcaggcaggt agcgcagctg aaggccccgc tcgacggcgg cgcgctcgag ttcgtcgcag
    10681 gtgggctggt cagccccctc gccatcgggc cggttgcaga tcaccgaccg gaatccggcg
    10741 tcggcgaccg cctgcatgtc ggcagccgcg atctgcgggg cgaccgaaag ctcgcccgtg
    10801 agtggtcgaa tgtccatggg acttgtctcc tgaaatcgcg gcctctcgag gaggctctgg
    10861 ggcgcgctgc ggtcagaccg cgttcaacgg aatcttgatg tagcgggtac cgttggcttc
    10921 gggctcgggc cactggccgg cgcgcatgtt cacctgcacc gacggcagga tcagcgtcgg
    10981 catgtcgagt gccgcatcgc gtgcgctgcg catcgccacg aactcctctt cgctgatgcc
    11041 gttgcgcaca tggatgttgc gctcgcgttc ctcgcccacc gtgctgacga actgcacctc
    11101 gcggccaccc ggctggtagt cgtggcacat gtagaggcga gtcgcggcgg gcaggctcag
    11161 caccttgttg atcgagcgga acaaggtgcg ggcgtcacct cccgggaagt cacatcgggc
    11221 ggtgccgtag tcgggcatga acagcgtgtc gccgacgaag gcaaccgtgt cctccccatc
    11281 gatgacgaca taagtcatgc aggccggcgt gtgccccggc gtgtgcatcg cacgcgcctg
    11341 caggccgccg atcgcgaatg cctccccgtc ggcaaacagg tggtcgaact ggcggccatc
    11401 gcgtgcgaac tcggctcctg cgttgaacag gctgccgaag acctcctgca ccttcgtgat
    11461 gtgctcgcca atgccgagct ggccaccgag ctccttcttc aggtacggcg ctgcggacag
    11521 gtggtcggca tgggcatggg tctcgagaat ccattgcacc tgtgcgccca attcacgcac
    11581 ccgggcgatc aggcggtcgg cgctcgccgt cgacgtgcgg ccggacttgg ggtcgtagtc
    11641 gagcacgctg tcgaccagtg cgcactggcg cgacggccgg tcgagcacga gatagctgac
    11701 ggtccaggtg ccgggatcga agaagccctc gatctgcaag ttgctgtcct tacccatggg
    11761 tttctcctcg ccccgcggcg ccttgcgcgc gggggcctcc aggttgcttc aacggcgtgc
    11821 cactgggcag gtgttgatgc cgaggaccgt gtacagcggg cacatgccga tcgcaccggt
    11881 gagcagcggc acgacgccga tatagcccca catgccaacg gttccggtcg cggccaggcc
    11941 gatcagtacc agaccgcctg ccacgcgcaa tgcccggtcg atggtgcctt cgttcttctt
    12001 catggtgtgt tcctttctag gtgggttcag cagcgctcgc gacgaccttc gccgcgactg
    12061 catgctgctg ttcattacaa gaggcgtgcc atccgtggca tttgcgacag atcaatgcca
    12121 aggcattgat ctgattgaat tttttccagg atggccgaat accgcagtcc gcctcgactg
    12181 ccacgcaacc gcagttggca gatcttcgct gccgaatttg gcagtcatgg cagttgggcc
    12241 cagaatgccc cggcaggagc acccgccatg gccgacaccc accgatccga tccgaacacc
    12301 gacccttcgg attccggcga cctgcattcg gacccccagg tgcaggccct ggtctcgttc
    12361 ctggagcacg aggcgcagcc catgatcgtg ctcgaccccg actacaacat cctggccgcc
    12421 aacacggcct accgtcggca gttcggcacc gtggacaagc cctacctcgg ccacaagtgc
    12481 taccgcatct cccaccacta cgacgtgccg tgcgaccagg ccggcgagca ctgcccgatg
    12541 agaaaggcct tcgaggccaa gggccccgac cgcgtgctgc acatccacca cacgccacgc
    12601 ggcccggaac atgtggacgt ggagttgcga cccatcctcg acggcgaccg gaacgtggtc
    12661 gcctatgtcg aacggttgtc gacggtgcgc agcgcgtccg cacgccccag cgcggaaggc
    12721 ttggtcggcg gttcgccggc cttcaacgcc gcgctgtccg cggtgcagcg ggtcgcaccg
    12781 gcgatgctgc cggtgctgct gctcggcgaa tccggcaccg gcaaggaact gttcgcacgc
    12841 gccgtgcacg aggccagcga acgcgcgcac ggccccttcg tggtcgtcga ttgctcgggt
    12901 ctcaccgaca cgctgttcga gagcgaagtc ttcggctacg agaagggcgc cttcaccggc
    12961 gcccacgcac gcaagcgagg cctggtcgag accgccgaag ggggaacgct gtttctcgac
    13021 gagatcggcg acgtgcccct gagcatgcag gtcaagctgc tgcgcctgat cgagtcgggc
    13081 acgttccgcc gggtcggcag catcgagacc cagaaggccg acttccggct ggtggccgcg
    13141 acgcacaagc cgctgcagca gatggtggca gacggccgct tccggcaaga cctgtactac
    13201 cgcatcagcg cgtttccgat ccacctgccg ccgttgcgcg aacgcgcgga cgacatcccg
    13261 atgctggtcg actcgttcct gcagcgaggc agctccagca agcatcgcgt gtcggtcgag
    13321 gcccgcgcga tggcacgcct gcaggcccac gactggcccg gcaacatccg cgagttgcgc
    13381 aacgtgctgg agcgagcccg cctgtttgca gacgatggca tcgtgcgcgc cgagcacctg
    13441 cccgaaggcc tgggctgcga aggcccagcg catgcagccc cggagcgcca cgcaccgccg
    13501 tcggaacggg tccgcgccgg tgccgacgat gccgagctga gacgcctggc caccagcttc
    13561 accggcaccc gccgcgagct ggccacccgg ctgggcatga gcgaacgcac gctgtaccga
    13621 cgcctcaagg ccatgggcct ggcctgagcg cggtcgggcc ggcgcgcacc gcgcctactt
    13681 caaccgggtg ccatgcaggt cgaagatgga taccttcacc ggaaaggcct ggctgacgct
    13741 ttgcgtcacg gtgcagtagt cctcgaaggt ggccagcacg cggtcgagat gctccagcgc
    13801 gtccgccggc acccccacgg tcagcgtgac ctgcatcgac aacacccgca accggccctc
    13861 gggactgcgc cccacttcgg cctgcgcttc acaggtgatc ggatcgggct gctgcttgaa
    13921 tttgcgcagt gaaaacagca acgaagcgct gaggcagctg ccgacggccg atgcgagcaa
    13981 ctgaaccggc gacggcccgg ttccctggcc cagcggtgcg ggctcatcga gccggatcgg
    14041 cggacgctct cctccgaaat cggcgtcgaa ctggtagtcg tgcacctgtg tcaggcgcac
    14101 aggctgtgtt gcagcggtca tggcgatctc cttcatcgaa cgggtgtgag tgacaggccg
    14161 cagcggttgt ctgcggactg tcggcgccgg cgccggcgcc cgagacggct cacagtctga
    14221 gccctctcgg gtggggcgtt cagaacctga ggctcatgcc gatgtcgaac gatctgccct
    14281 gccccggcag cggttcgatc tgcgtcgccg acgcagaagc cttgtaggcc gccaggttgg
    14341 cgccgcccag cggcaggtcg tactgccgat cgaacaggtt gcgcacgccg gcctgcacgg
    14401 agaacgcctt gttcatctgg taccgcgcac ccagattcac caggctgtag ccagctgttc
    14461 gatcctcgtc acggcgatcg tcgaccgccg acttgcgagc gaccgcctga agctcggcgg
    14521 acagcgtcaa gtcgcgccac cgctcttcca ggcccagcga caggttgacc ggcatctggt
    14581 gatagaggtc accgccgtcc tcgcgccggc cccgggtcac atcgagcttg cccttgaccg
    14641 tgccggtacc ccacgacgcg tcgtcccagg cgaccgccga ccaggacagg ttgacgccgt
    14701 agaggtgtgc atcgtggttg gcgaactgga gcaacgcctt cgacgccccc atgcgcatgt
    14761 agggcttgaa ggtcccgatc acattgacgt cgatgtaatc ctcgacatag gtgtagtaag
    14821 gcgtgaggga gacttcccat cgcgccttcg ctgcatcgtg ccagtttacg gtggcgctca
    14881 cggtgtgcgc cacttcgggc ttgaggtcga tgtcgcccac gtagccgttt ccatcgccga
    14941 accagcccac catcatcatg gccatcatgc cgcggcccca gctgtagcgc tcgtagaggt
    15001 tcggcgagcg ctccttgcgg gcgaacccca gctggtactc gacctcctgc gaggacgcat
    15061 aggtggccag cgccgtcaaa tcgatgttgt ggtcacgctg cttgcggctg cgtgcattga
    15121 atgcggccgc agcggcattg tccggagcgc acatcattcc gcagccgtaa ccctgcacgt
    15181 cgcccgcatt gctgcgaacc gactcgtggc gcacacccag cagcgtgctc cacttcgcgc
    15241 cgagccgggc ttcccattcg gcaaacagcg ccatgcggtc cctacgacca tcgacgatgt
    15301 tttcgtaggt gttcggggac atcatcatcg agccagccac cggcggccac cagtcgtcga
    15361 ggcggaaccg atggaattca ttgccgatgc gcagcttgcc gtcctgccac ggcagctccg
    15421 cctgcagctt gtagcccgca tcccgaccgt gcgttttcat gggcatggtg ccggtcttct
    15481 cgcgcgtgaa gaaccccatc tcgtgccgcg tgtcctgccc atagatcttg gcttccaggc
    15541 gcccccagtc gaagctgcct tcgtaggcca gattggcaaa gaccccgcgg ttgtccgtca
    15601 tgtccatgta ctggttcgga tagccctggt acggaatgga ctgccgcccg accttgaaga
    15661 ccagacgttg accttcaccc tgcgcggcca gcgtcagggc gtggttgttg ctcttgtaga
    15721 gggtatcgag caccttgtcg ccgtgcccgt cgcggtagct ttctcccttg gccagggcgc
    15781 cgctgtactg gaggctcaac gtttcgctcg ccacgccagc ggaaagttgc agcgtgcgac
    15841 tgtcgtcgac gctgcgcctg aggaacgcga tctcgccctg ggtcagcagc ccctcgcccg
    15901 cccgcgcgaa gaccggagcg gcggattcca catcgatcac gccaccgatg ttgtcgcccc
    15961 ccaggctgac cggcgaaatg ccggcgaccg cccgggccct gctgacctgg ctgggctcga
    16021 tgtaggagag cggggcgttc atgtgattgg cacacgccga ggtggtttcg gccccgtcga
    16081 tgcggatctt caggcggtcg tctgccagtc cgcgcagggc aggcagaccg gagactccgc
    16141 ccgcgctgta ggtgctggcg ccgggctggc tgttcaacag ggtggcactg tcgctggtcg
    16201 acaggctgcg gccggctgat gcatcggagg cgaccgcgcc ccggatcacg acgggtggca
    16261 ggacggcctt gtcggcggcg gtcgccatga agggctgaag gccgatggcc aacaagatga
    16321 ggctgttgcg tttcatagaa cagtgatgtc cggaaaatat gaatgggaaa aggaaggcct
    16381 cgcgcatcgg ggtgcggcaa gcagaccgca cgcggaacga cgcgctccgg agggccgcgg
    16441 tcgatcgcgg cggtctgccg ggcagaagac agggacaagg gggatgaagc cgaccggccg
    16501 aggccccgca gcgacatcca ccggccagcc ggcggtgcgc aagcgcggat cggcgcggca
    16561 aggcgccgcc ctggctacga cgaggacgtc agcaggtcag gaaatgcgag gggggggcgc
    16621 ggggctgcgc gctggcccac gcatggagcg tgtgcttcgc ccgcagaaac agtcgaggca
    16681 cctcgaaccg cagagtgtcc ggcaagcgga ccaccagcgg ctcgctgggc aagagtgaat
    16741 cgtgaccctg gcaaatgcag aaagcgcagt gcgcgaggtc tcccaattca gaccggggag
    16801 caggcgaacc ggaggactcc cgtccggagt catcgactgc ttcgacaccc gtccagagca
    16861 cccctgaact gctgcagatg gcgcccaccc cggcatcgcc tgccgtgtac aaggcgtgtg
    16921 agacggtggg cgccagactc gcgaaccaga cgacgaatat cgcgatccag cgtgtgcaga
    16981 tgttggtgag caggcgcgag cgcatgggga aaagtctatt ccctcgcacg tgcgattcgc
    17041 ttgacccacg tcagttcggc ggagccggat cagcggttca gctgcgcata cacctcgctg
    17101 accagtcctc ggacctcggc ctcgctgccg tcagcaatga gtgcataacc gtagcgttcc
    17161 tcgacccagt agaacgaggt tcgcgttccg tcgcgcacca gacggaacgc ggtctcggac
    17221 ggcgccgcat ccgcagtgaa ggcggcgacg aacagcgtca cccgcgagcc ctgctggttc
    17281 tcgtacatga actgcgctcg cggcgtgccc tcgcccggca gcagccgacc gccgagcagg
    17341 tggtagccgt gcgcgcccag cgacggcgcc ttgagcggcg cgccgagccg acggctcagc
    17401 cactgcacga gatgggcttc gtcggcagcc gtgacttcga ccggatggcg cttctcgggc
    17461 acgaagaccg catgcgcagc cgcggcctcg cgcacgaaca gcggggccgc ggccagcgcc
    17521 gagcccgagc cgggcggccg gccaccttcc accgaccgtt cgatggccat ccccgccatc
    17581 aggccgacag ccagcagcac cgcggcagcc gcaccttgct gccagcgggc aagccgtcgc
    17641 gcatgggttg ccgcacgcat caccccgtcg aggagcgccg gcggcgtgtc gcctacatcg
    17701 accgaccgtg ccaactgccg caactgggcg cgctgcagtt gccactgcgc cacgcgggcc
    17761 gcgtcatcgg gatgggactg cagccacgcc agcaccgccg ccacgcggtc ggcatcgagc
    17821 tgcgcatcca cgtaagcatg cagctcgtca tcggtcacgg cttcgggcgc ggtcttcatg
    17881 gcttcaccag ttgcagtgga tgcgcacggg gcgtctcgcc ggggtcgctc atcagttcgc
    17941 gcagccgctc gcgcgcgcga tgcaggcgtg acatcaccgt gcctacaggc acgtcgagga
    18001 tgcacgcagc ctccgcgtag gtgtactcct cgacggtcac cagcagcagc acttcgcgca
    18061 tcgacggggg caacgccgcc agcgcgcgct gcaggtccag tcgctcgccg aggcgcgcca
    18121 gcggctcgtg cgccggttcg ggcacgtcgt cgagcgctgc atggccgtcg tcatgacgcc
    18181 agtcgcgtcg atggttcagg tgcaggttgt gcatcagcga cagcagccag ctgcgcagca
    18241 ccgagcccgg ctcccacagc gaccacttga cgcaggcgcg ctcgagcgtg tcctgcacca
    18301 ggtcatcggc gcgcgcggca tcgccagtca gcaaccgggc ataccggcgc aacgccggta
    18361 tgtggccgag ggtctgttcg atgcccacgc gcaacgagac cgatcgccgg actcagggcc
    18421 gtgcgaggtg ccagacgttg ttgacgccat cgccggtgcg gtcgcccgcc ttctggtctg
    18481 ccgcccagaa gtaaaccggc ttgcccttgt aggcccactg ccggctgccg tcgtcgcgga
    18541 tcaccacgct ccagtcgccg cgggccacgg catcggacgc ggccgcgagc ggcggccatt
    18601 tggccgcgca ggggccgttg cagacgctct tgcccgagcc ggcggcgtcg cggtcgaagg
    18661 tgtacagcgt catgccgttg gcaccgacga gcacaccatc ggcttcctgg gccggcgcgg
    18721 cctgggccat cacggcgctg ccactggcca gggcagcaca gaaaacgagt gcagacagaa
    18781 acgcaggtcg ggtcatggaa atctcctcga gtgggggggg tgacatgacg gtgaacaccg
    18841 cagcggccgg aagtattcca tccgttgcgc gaaggtcatg ctccgatcct ctgctccgca
    18901 tgggcgctgc cactgccatg gccgcgccag acaactgcca gcccccgcca tcttggcagc
    18961 ttcgcgtggc cgaagttggc agacggacac catcaagaaa tcacaacccc ctgtcgcaca
    19021 acaggttttc cgaatgccag gcgatggcac ggaacttggg ttgatcaggg catgacaccc
    19081 gatgaacgcc gcctggtacg ccacctcgtc actgccgtgc tcgtcaagct ggcggtgctg
    19141 gtggggctgt ggtggggatt cgtgcgcgat cagcgtgtcg agacggacac cgcacggacc
    19201 gcggcacacc tcggtgccac aacccccgat cccggagcca ccccttgata tccgaacaac
    19261 tcgtcgatct gtcgcgcctg cagttcgcgg ccaccgcgat gtaccacttc ctgttcgtgc
    19321 cgctcacgct cggcatggtg tggctgctcg tcatcatgga aagcgtctac gtgatgaccg
    19381 gcaaggtcgt ctggaaggac atgacgcgct tctggggcaa gttgttcggc atcaacttcg
    19441 cgctgggtgt caccaccggc atcacgctcg agttccagtt cggcaccaac tgggcctact
    19501 actcgcacta cgtgggcgac atcttcggtg cgccgctggc catcgagggc ctgatggcct
    19561 tcttcctcga atcgaccttc atcggcctgt tcttctttgg ctgggatcgc ctcacgcgca
    19621 cccagcacct gatggtgacc acgctgatgg cggtgggcac caacctgagc gcgctgtgga
    19681 tcctgatcgc caatggctgg atgcagaacc cggtgggtgc cgagttcagc taccagacga
    19741 tgcgcatgga gatggtcgac ttctgggcga tcgtgttcaa cccggacgcg caggccaagt
    19801 tcgtccacac cgtgtcggcc ggctacgtga cgggcgcgat gttcgtgctg tcggtgtcga
//
LOCUS       DAOORV010000065        19531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3405_length_19531_cov_5.888735, whole genome shotgun sequence.
ACCESSION   DAOORV010000065 DAOORV010000000
VERSION     DAOORV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_19531_cov_5.888735"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKV56_10135"
     CDS             complement(<1..698)
                     /locus_tag="PKV56_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOX68213.1"
                     /translation="MSALKAATIETSPLVEVRNLSKTFVVPVPFLERMIERRPLPLVR
                     AVDDISFAIERGQTLALVGESGCGKSTVARLLVGLLNPTAGGVLFDGQYLSSAFAPGR
                     ASPQRKRVQMIFQDPYASLNPRWRVADIVGEPLVEQGWLSGRLALRERVAELLALVGL
                     AAEDAGKLPHQFSGGQRQRISIARALATNPEFLVCDEPTSALDVSVQAQVLNLMRDLQ
                     RSRGLTYLFISHNLA"
     gene            complement(695..1669)
                     /locus_tag="PKV56_10140"
     CDS             complement(695..1669)
                     /locus_tag="PKV56_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68214.1"
                     /translation="MSPLLEVDRLCVDLGSRHGSLRLLDDVSFAIAPGEMLGFVGESG
                     AGKSLTGSAVIGLLEPHCHMTGGRIVYDGRRIDDLPDDEVRRLRGRQIGAIFQDPLTS
                     LNPLYTIGHQLVETMRTHLALTEAEAISRAVDLLEETGIQGAAQRLRQYPHQLSGGMR
                     QRVVIALALAAEPRLVIADEPTTALDVSVQAQVMALLRRLCRDRGMAVMLVTHDMGVI
                     AESCDRVAVMYAGRIVEIGPVRDVIQAPAHPYTAGLMGSIPPMTGERGRLVQIDGSMP
                     RPGLIPSGCAFHPRCSQGWDRCRIERPDLMAVGASRSACWLHDPRRTG"
     gene            complement(1666..2595)
                     /locus_tag="PKV56_10145"
     CDS             complement(1666..2595)
                     /locus_tag="PKV56_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68215.1"
                     /translation="MSDAVTSPGPLRRFLDGDLWYSFTHTPTAIVAASIALACIVVAA
                     FTNTLAPHHPFDLATLELADSRLPPAWLPEGRSTYLLGTDDQGRDILSALMYGSRISL
                     LVGLASVVLSTIFGVTLGLLSGYLGGKTDAVIMRICDVMLSFPAILLALMIDGVGRAL
                     FPQAHDALAYAVLIVAISAAGWVPYARAVRGGAMVERQKEYVQAARVIGVSPLRIMRR
                     HVLPNVLGPVMVLATLQLATAILIEATLSFLGVGVPPTSPSLGTLIRIGNDFLFSGEW
                     WITVFPGAMLVLIALSVNLLGDWLRDALNPRLQ"
     gene            complement(2592..3581)
                     /locus_tag="PKV56_10150"
     CDS             complement(2592..3581)
                     /locus_tag="PKV56_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68216.1"
                     /translation="MLVFIVRRLAQAVLVMLTVALVSFVLFQFVGDPVTNLLGQDATP
                     GQREQLRRDLGLDQSAPTQFVRFVGNAVQGDFGLSLRQGRQVSSLIAERLPATLELSL
                     VAAVLALALGVPLGVYAALRRDGFWAHCLMAVSLLGVSLPTFLIGIGMIMVFSVTLNW
                     LPSFGRGEVVRLGAWSTGLLTADGWRHLVLPATTLAIFQLTLIMRLVRAEMIEVLRTD
                     YIRFARARGLTNRSVYFGHALKNTMVPVITVTGLQLGSLIAFSIITETVFQWPGMGLL
                     FIQAVQFADIPVMAAYLCLVALIFVLINLAVDLLYFAIDPRLRAAGFGEATVP"
     gene            complement(3654..4406)
                     /locus_tag="PKV56_10155"
     CDS             complement(3654..4406)
                     /locus_tag="PKV56_10155"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOX68217.1"
                     /translation="MNAVRRRGGARAAFRVVAVVLGLGVAGCESMRDVPPPRAPAQSD
                     VAWKPFVLPTKRETRYVRSVIDGRPVIQADADNAVSLYRRAMRIEPEKLGHLSFSWMV
                     PKLIDTADLSIQSSEDAPVRIVLAFDGDRSKLSLKNRMLFDLLEAVIGEEPPYATLMY
                     VWDSRAPLESVILSARTDRIRKIVVDSGSDQTSIWRFHERDVVGDYRRAFGEEPGALV
                     SVALMTDSDNTRTTVRAYYGDVRFSGRDGAMR"
     gene            complement(4411..5673)
                     /locus_tag="PKV56_10160"
     CDS             complement(4411..5673)
                     /locus_tag="PKV56_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX68218.1"
                     /translation="MKRHLLVFVILASVQSSGHAQSKLELYGIVDAGVTRVSGVKGGS
                     VTQLSSGIMEGSRIGLRANEDLGNGYRAIFTLENRLEADTGDISNRPPSRGQLPDRAT
                     FARHLLPSLPAAQQSALQPLLNQVSAGIGTRVGINVHAAGPNFFDRQIYVGLVTPVGA
                     VLAGRMYTPGYELAGTFDALSAQSSLSSGQIAAVPYAIDIRSSNALAYRLQQGPWTAS
                     MMYGFDEKTTETGRFAGVLGMYRSDAFGLGVAYNNRRNEKGQQSLTSLVFGAYLNFGS
                     GQFYASAASIRDGHPSAVSGIAVPITPLIGPALAALVQAGYTDALKQDAKLYHLGYKH
                     VSGPGTVYVACNLYDDSKAANADVASFGVAYSYALSKRTDLSAIATRFNNTGLGQAAP
                     GGGGYLGGVTSHAGRDSTSLALGLRHRF"
     gene            complement(5839..6948)
                     /locus_tag="PKV56_10165"
     CDS             complement(5839..6948)
                     /locus_tag="PKV56_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX68219.1"
                     /translation="MKKSLVAVAVLGGFASVAVAQSSVTLYGRIDQNITYQDPGKNAA
                     ASSGGRLGKGVTKLNDGGVSGFGNSRWGLRGSEDLGDGLRAYFLLESSIAADTGAGGG
                     STVSGSSFFNRQAYIAIGSKTLGDIRLGRIETISRETNVRINDATGENEIGIQEVVAS
                     NRPLFQNFGTRIDNGVSYRSPVLGGFQGILTIGLNEDFKSTSSATPPVTSKASEYHGV
                     GLIFGQGPFNAAVSYEEFSGGVVSGSYNKAVTVGANYNFGLATVYAAYQNTSDFGPQL
                     AAPTFAKGTDHDAYNVGVKVPYGNFTFKAQYTSSTVDRTGALSDLDQEKYGLSAAYAL
                     SKRTSVYAAVTERSGDLNRNFARRNEYVLGLGHNF"
     gene            complement(7150..8370)
                     /locus_tag="PKV56_10170"
     CDS             complement(7150..8370)
                     /locus_tag="PKV56_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX68220.1"
                     /translation="MEYPDPRVPCMSICRLIGRSGFRFSFFRFLFPVQESLQMKKSLI
                     ALAVLGTFSTIAAAQSSVTLYGRLDENVTYQDPGKNAAASSGGRLGKGVTKLNDGGVN
                     GIGGSRWGMRGSEDLGDGLRAYFVLESSISGDTGAGGGATLSGSGFFNRQSYVAIGSK
                     TLGDIRLGRVETMTREMAVRVNDVTADGELNITEVVATGRPLFQNFGSRVENGISYRS
                     PVIGGFQGLLTVGLSEDFKSINATTGAVSKAAEYRGLGLVFSQGPLNASLTYEELSGG
                     AASGSYNKVVTLGANYDFGVAAVYGAYQDTSDFGAQLASTTFAKGTDHDAYNLGVKVP
                     FGNFTFKAQYTSSTVDRSGGLSNLDQDKYGASVAYSLSKRTTVYAAITERGGDVKESF
                     ARKNEYLMGLAHTF"
     gene            8623..9267
                     /gene="coq7"
                     /locus_tag="PKV56_10175"
     CDS             8623..9267
                     /gene="coq7"
                     /locus_tag="PKV56_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041388313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HOX68221.1"
                     /translation="MLDSLIITADQALRTLSGGVTAARPIPRHLESGPTSIAEGDSAL
                     SEAEKSLSGALMRVNHVGEVCAQALYQAQAIGTSDPAMRQLLEAAARDETDHLAWTQQ
                     RLGELGARVSLLNPLWYAGSFTLGWLAGRMGDKWSLGFLVETEKQVEQHLAGHMNRLP
                     ATDQRSRAIVQQMKLDEARHAGEAQQAGAAELPVAARWGMRILAKAMTRTAHYL"
     gene            complement(9278..9700)
                     /locus_tag="PKV56_10180"
     CDS             complement(9278..9700)
                     /locus_tag="PKV56_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX68222.1"
                     /translation="MECTVNWVPGLGMGFVAETGSGHVLVMDGAAEGGGRNLGPRPME
                     TVLAGTAGCTAYDVVLILKRGRHAVSGCQVRLTSERADTDPKVFTKIHMHFRVSGTDI
                     PAPALERAIQMSHDKYCSASIMLGKTADITTSYEIAAS"
     gene            9981..11612
                     /gene="ilvA"
                     /locus_tag="PKV56_10185"
     CDS             9981..11612
                     /gene="ilvA"
                     /locus_tag="PKV56_10185"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOX68223.1"
                     /translation="MSGRNKSEAVPRPTESGALGTSIRTASKKSGKGTTRAIPLSVSD
                     YLQKILTARVYDVALETPLELARSLSKRIQNQVFLKREDCQQVFSFKLRGAYNKMAHL
                     SAEQLQRGVICASAGNHAQGVALGAKRLGCKAMIVMPVTTPRLKIDAVKSLGGSVVLH
                     GESYSDAYAHALELEQKHDLTFVHPFDDPDVIAGQGTIGMEILRQHPDKIDAVFVAIG
                     GGGLISGVAAYIKAVRPEIKVIGVQMNDSDAMIRSVQAGKRVILSDVGLFSDGTAVKQ
                     VGVETFRLTRELVDGFITVDTDAVCAAIKDVFQDTRSILEPAGALAVAAVKQYVETHQ
                     VKGQTLVAIACGANMNFDRLRFVAERADVGEEREALLAVTIPEERGSFKRFCQVIGPR
                     SVTEFNYRISDERKAHVFVGLATTQRGDSATLAASFNSQGFQAIDLTHDELAKLHLRH
                     MVGGRSELASDERLYRFVFPERPGALMKFLSSLHPGWNISLFHYRNQGSDSGRILVGM
                     QVPHKDKKAFRDFLGTLAYPCVDETDNPAYRLFLR"
     gene            11675..12748
                     /locus_tag="PKV56_10190"
     CDS             11675..12748
                     /locus_tag="PKV56_10190"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOX68224.1"
                     /translation="MSSYHNLISPTGNTQLELALREKLLRRSQTTGELGELEPLAVRL
                     GLIQRTLKPRFQAPQIVVFAADHGIAVDGVGSEDRNSTSVLVQALLTSRLPLSVFARI
                     QGLELSVVDAGVAEPVSPHPRLLARKIAHGTRNSRVTSAMSLEQAHAGIRAGMEIGNS
                     LPGNAVVCAGIGVGSAQASALVLSRLANVGVREFAVSGPSMKPKDLANMLAVLQGSQN
                     RHKEVSDPVEVLAAFGGFEMAMMVGLMMVAAGKRHLIVVDGLAACAALVVGSHIAPTV
                     VDYCVFSRSHAHLGLDRALALFQARALLELGMDSTDGTGATLAWSLIRGAAALLTEVG
                     EGEEAGPTLPGDMPEVSRPRPNY"
     gene            complement(12762..13307)
                     /locus_tag="PKV56_10195"
     CDS             complement(12762..13307)
                     /locus_tag="PKV56_10195"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOX68225.1"
                     /translation="MVSRLSALLVWALVAGSAVFWGLRLLGQAAPSDALALASSEQVG
                     AQGDLTRLFGAASVEVTDEVLDAPAPDISARFRLTGVIAPKRPSEQGLAVISVDGNPA
                     RVYRVGAVIEGEWILREVSLRTATVALARNAGDAAAAFVLELPLVANAVPGVLPPALP
                     FTEPTSGAVMPGPTHRSVTMR"
     gene            13366..13845
                     /gene="gspG"
                     /locus_tag="PKV56_10200"
     CDS             13366..13845
                     /gene="gspG"
                     /locus_tag="PKV56_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOX68226.1"
                     /translation="MFDTDSDPALQRHPTGPHRSTRGALACGFTLIELMVVLVIIGVL
                     AALIVPNVLNRADDARVTAARTDVNNLMQAMKLYKLDNQRFPSADQGLQALVAKPTVG
                     VIPPNWKPYLDKLPNDPWGHPYQYANPGVKGSIDIYSLGADGVVGGEGNDAEVGSWQ"
     gene            13859..14323
                     /locus_tag="PKV56_10205"
     CDS             13859..14323
                     /locus_tag="PKV56_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOX68227.1"
                     /translation="MRSRCSGFTLIELMIVIALIALAASVVSLALRNPADNQLEQEGA
                     RLAALLESARAEARASGLAVQWVPRSSSGNAPAASGFTFVGLPASAGLPQTWLFAGVT
                     AAVIGAPAVLLGPEPMIGAQRITLSLEDRRLTLATDGLGPFIVTSDEPAAPR"
     gene            14323..14724
                     /gene="gspI"
                     /locus_tag="PKV56_10210"
     CDS             14323..14724
                     /gene="gspI"
                     /locus_tag="PKV56_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOX68228.1"
                     /translation="MQLIKLAGGRLLHRKQAEHGFTLIEVLVALVIVAITLGAGIKAA
                     GALLSNAQRLTEVSEAQWCADNELTELKLAKAYPGIGESSFNCEQLGRSYVGTFQISA
                     TPNPNFRRIEARIANEAGETMLTLSTILPRY"
     gene            14724..15386
                     /locus_tag="PKV56_10215"
     CDS             14724..15386
                     /locus_tag="PKV56_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOX68229.1"
                     /translation="MMDCQASRQSGFTLVEVLVALVVMATLALMAWQGVDGIVRTRDA
                     SQSRLEATLRLGTVIAQWEQDLASIQETDVVPALTFDGSTLRLTRRTDDGIQLVAWSL
                     QPSTGPEGVRNQWLRWAGPSATSSGPLQESWLRSQSLQGNPQGASRTLTGISQWQVYC
                     YRGNAWTNCQSSGDVAATAGGAGPAKVVLPSGLRLVLTFSGGDGFDGTLTRDVAFGPQ
                     AQ"
     gene            15383..16357
                     /gene="gspK"
                     /locus_tag="PKV56_10220"
     CDS             15383..16357
                     /gene="gspK"
                     /locus_tag="PKV56_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOX68230.1"
                     /translation="MRSKTFIGRPLQRQAQQGAALLTAMLIVVLVTTLASAMVWQQWR
                     AVQVEAAERARTQAAWILAGALDWARLILREDARSGSATTLNDPWATPLAEARLSSFL
                     AADKAHTDDGPEAFLSGAIADAQSRYNLRNLVDDTGKVIPAEAAGLQRLFESVGVSPD
                     LATRITSGLRGALAPAGSEDRPASPPLMPRTVSQLTWLGVDAASLRRLAPYVVILPVR
                     TPVNLNTAPREVIAASVEGLDVGVAERLVQARKRTPFKTVADAQAALPDNLTLDTNRV
                     GVLSNFFEVRGRVRLGDRMLEDLSLVERRGLDIVPLQRQRLSLRDTAG"
     gene            16493..17752
                     /gene="gspL"
                     /locus_tag="PKV56_10225"
     CDS             16493..17752
                     /gene="gspL"
                     /locus_tag="PKV56_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOX68231.1"
                     /translation="MSILVISLAQRPRIRARSASLDEAVTGPSHADDDIEHVFSRDGF
                     SIQAQGRCAAASLPKADSVVAVVGEADIAWHRITLPKAPAGRLRAALSGVLEEAVLAD
                     MEATHFALAPGSAAGQPTWVAAIDRAWLQEELARLERHQVFVDRIVPLAWPDDSPSGH
                     FAEASGTGEPEEATLTWAHSGGVAKLRLQGSLARSLVPQPAEAGTRWTATPEVATVAE
                     RWLGAPVLAISKGERALQATRTLWNLRQFDLAARHRGTRAVRDLARQWLSPAWRPTRR
                     GLLVLVAVQVVGLNLWAWHQSAEVARQKQAMVAVLQGSFPQVRAVLDAPLQMQREVEA
                     LRAQAGKASETDFEPMLYAAASAWPETQPQVNSMTFEPGRLTLAAPGWADGEIEQFRV
                     QLRQAGWNVDAANGQVSLSRLAAGLTP"
     gene            17749..18312
                     /gene="gspM"
                     /locus_tag="PKV56_10230"
     CDS             17749..18312
                     /gene="gspM"
                     /locus_tag="PKV56_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOX68232.1"
                     /translation="MSGPAEKALPAASSAEASPRPGTTPASTRVLGRQLEARWAALAP
                     RERVWLKGAGAVIVVFMLWVLAIQPAWRTARAAPAQLDRLGIQLQTMQQLGAEATDLR
                     NSAPVSAAQAGTALQAATERLGSRAQLLMQGERVTVNVKGLSGNDLIDWLAEARSGAR
                     ARATEVRLARDAQGYSGTVVLSLGSGR"
     gene            18498..19190
                     /gene="gspN"
                     /locus_tag="PKV56_10235"
     CDS             18498..19190
                     /gene="gspN"
                     /locus_tag="PKV56_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOX68233.1"
                     /translation="MLADARGTVWSGSATAVLTGGPGSRDSSALPGRLFWSVQPQGLA
                     LKVTLSQACCIEGRVGVLLKPGWSGFSAMLQPETDRIGQWPSRWLTGLGTPWNTLKLG
                     GTIYLQSSGLTLESTNGHWRISGGMDLELIDASSSLSTLEPLGSYRLALQGTPPSEAP
                     DPQTGSTESALLTLSTLEGALLLSGNGTWGAKGLNFRGEATARDAGDEALTNLLNIIG
                     RRDGARSVISIG"
     gene            19211..>19531
                     /locus_tag="PKV56_10240"
     CDS             19211..>19531
                     /locus_tag="PKV56_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HOX68234.1"
                     /translation="MSSLSRWLRRHATRLGAGTASLPLTLSLVATPLGTLPAPARAQA
                     AAGEAVANRFRGEPVTLNFVNADIDAVSRAIAAILRQQFIVDPRVKGTMTLFSEQALP
                     PREDY"
BASE COUNT         3728 a         6350 c         6210 g         3243 t
ORIGIN      
        1 gcgaggttgt gcgagatgaa gaggtacgtg aggccacggc tgcgctggag gtcccgcatc
       61 aggttcagca cctgcgcctg caccgagaca tcgagcgccg aggtcggttc gtcgcagacc
      121 aggaactccg gattcgttgc cagagcccgg gcgatcgaga ttcgctgccg ctgcccgccc
      181 gagaactggt gcggcaactt gcctgcatcc tcggccgcca gacccaccag agccagcaac
      241 tcggcaacac gctcgcgcag ggcgaggcgg ccagacagcc acccctgctc gaccagcggc
      301 tcgccaacga tgtcggcgac tcgccaccgc gggttcaggc ttgcgtatgg gtcctggaaa
      361 atcatctgca cccgctttcg ctgcgggctc gcccgacctg gcgcaaaagc cgaagacagg
      421 tactgaccat cgaagagaac gccacctgcg gtcgggttga gcaggccgac cagcaggcgc
      481 gccacggtgc tcttgccgca ccccgattcg ccgaccagag ccagcgtctg ccccctctcg
      541 atcgcgaagc tgatgtcatc gaccgcgcgg accagcggca acggtcggcg ctcgatcatc
      601 cgttcgagaa acgggaccgg cacgacaaag gtcttgctga ggttgcgtac ttcgaccaag
      661 ggcgaggtct caatggtcgc ggctttcaaa gcgctcatcc agttcgcctc gggtcatgca
      721 accagcaggc cgagcggctg gcccccacag ccatcaggtc aggccgctcg atgcggcagc
      781 gatcccagcc ttgcgagcag cgcggatgaa acgcgcatcc cgacgggatc aggcccggcc
      841 tcggcatcga accgtcgatc tgcaccaacc gaccacgctc cccggtcatc ggcgggatcg
      901 aacccatcag tccagcggtg tagggatggg ccggtgcctg gatcacgtca cgaaccggac
      961 cgatctcgac gatgcgcccc gcatacatca ccgccactcg atcgcaggac tccgcgatca
     1021 cgcccatgtc gtgcgtgacg agcatcaccg ccatccccct gtcacgacac agtcgcctga
     1081 gcaatgccat cacctgtgcc tgcaccgaga cgtcaagcgc agtcgtgggc tcatcggcga
     1141 tcaccagcct cggttcagcg gccagagcca gagcaatcac aacccgctgc cgcatgcctc
     1201 cggacaactg atgcggatac tgccgaaggc gttgtgcggc accctggatg ccggtttcct
     1261 cgagcaggtc cactgctcgc gagatcgcct cggcttcggt cagcgcgagg tgggtacgca
     1321 tcgtctcgac cagctgatgt cctatcgtgt agagcggatt cagcgacgtc agcgggtcct
     1381 ggaagatcgc gccgatctgc cgacctcgca ggcgccggac ttcatcgtcg ggcaggtcat
     1441 cgatgcgccg gccgtcgtag acgattcgtc ccccggtcat gtggcagtga ggctcgagca
     1501 atccgatgac agccgagcct gtcagcgact tgccggcacc ggactccccc acgaagccca
     1561 gcatctcacc gggagcgatc gcgaaggaaa cgtcgtcgag caagcgcagg ctgccatgcc
     1621 gagatccgag atcgacgcac agtcggtcta cctcgagcag cggactcatt gcaggcgcgg
     1681 attcaatgcg tcgcgcagcc agtcacccag caggttcacg ctgagagcaa tcagcaccag
     1741 catcgcgccg ggaaacaccg tgatccacca ttcgcccgag aacaggaagt cgttgccgat
     1801 gcggatcagc gtccccagcg aaggtgaagt cggcggcacc ccgacgccga ggaacgacaa
     1861 ggtcgcctcg atcaggatcg cggtggcgag ttgcagcgtg gccagcacca tcaccgggcc
     1921 caggacgttc ggcagcacat ggcgacgcat gatgcgcagg ggtgagacgc cgatcacgcg
     1981 cgcagcctgc acgtactcct tctgccgctc gaccatcgca cctccacgca cagcgcgcgc
     2041 atagggcacc cagccggccg cagagatcgc gacgatcagc accgcgtagg ccagcgcgtc
     2101 gtgcgcctgc gggaacagcg cacggccgac accgtcgatc atcagtgcca gcagaatcgc
     2161 cggaaacgac agcatcacgt cgcagattcg catgatgacc gcatcggtct tgccaccgag
     2221 gtagcccgac agcagcccga gcgtcacgcc gaagatcgtc gacagcacga ccgacgccag
     2281 accgacgagc agcgagatgc gcgatccata catcagcgcc gacaggatgt cgcggccctg
     2341 gtcgtcggtg cccagtaggt aggtggagcg cccctctggc aaccaggccg gtggcagcct
     2401 cgagtcggcc agttcgagag ttgccagatc gaagggatga tgcggcgcga gcgtgttggt
     2461 gaacgccgcg acaacgatgc aagcaagcgc gatcgaagcg gcgacgatcg ccgttggtgt
     2521 gtgggtgaag ctgtaccaga ggtccccgtc aaggaagcgg cgcagcggac ccggcgatgt
     2581 gacggcgtcg ctcacggaac ggtcgcttcg ccgaaacctg cggcacgcaa ccgcggatcg
     2641 atcgcgaagt aaagcaggtc gaccgccagg ttgatcagga cgaagatcag ggcgaccagg
     2701 cacaggtagg cggccatcac cggaatatcc gcgaactgca ccgcctggat gaacagcagc
     2761 cccatgccgg gccactggaa gaccgtctcg gtgatgatcg agaaggcgat caacgaaccc
     2821 agttgcagcc cggtgaccgt gatcaccggc accatcgtgt tcttcagcgc gtggccaaag
     2881 tagacagacc ggttcgtcag accacgggcc cgtgcaaagc ggatgtagtc ggtgcgcagc
     2941 acctcgatca tctcggcacg aaccagccgc atgatcaggg tcagctgaaa gatcgcgagc
     3001 gtggtggccg gcagcaccag atgccgccac ccgtccgccg tcagcaggcc ggtgctccag
     3061 gctccgagcc gcaccacctc gccgcgaccg aaactcggca gccagttcag cgtgaccgag
     3121 aacaccatga tcatcccgat gccgatcagg aaggtcggaa gcgacacgcc gagcagcgat
     3181 acagccatca ggcagtgcgc ccagaaacca tcgcgccgca gcgccgcata gacacccagc
     3241 ggaacaccca gtgccagcgc cagcacggcg gccaccagcg acaactccag cgtcgccgga
     3301 aggcgctcgg caatcagcga tgaaacctgg cggccctgcc tcaggctgag cccgaaatcg
     3361 ccttgcaccg cgttgcctac gaagcggacg aactgagtgg gggcactctg gtccagaccg
     3421 agatcgcgac gcaactgttc acgttgtccg ggcgtcgcgt cctgcccgag cagattcgtc
     3481 accgggtcgc cgacgaactg gaacagcacg aacgacacca gcgccacggt gagcatcacc
     3541 agaaccgcct gggcgaggcg gcgaacaatg aagaccagca tggaggaggt ggggtgtcgg
     3601 acggaaatca gcaagaagcg cagattctgg cgcgcggcca cggaaactgc ttctcagcgc
     3661 atggcgccgt cgcgaccgga aaacctgaca tcgccatagt aggcgcgcac ggtggtgcgg
     3721 gtgttgtcgc tgtcagtcat caaggccacc gaaaccaggg ctcctggctc ctcaccgaag
     3781 gccctgcggt aatcgcccac gacgtcccgt tcatggaaac gccagatcga tgtttgatcc
     3841 gaacccgagt cgacgacgat cttgcggatg cggtcggtac gcgcggacag gatcaccgat
     3901 tcgagcggcg cccggctgtc ccagacatac atcagcgtgg cataaggagg ttcctcgccg
     3961 atcacggcct ccagcaggtc gaacagcatc cggttcttga gggacagctt gctgcggtcg
     4021 ccatcgaacg cgaggacgat gcgcaccggt gcatcctctg aactctgaat gctgaggtca
     4081 gcggtatcga tgagcttcgg aaccatccag gaaaacgaca ggtgccccag cttttcaggc
     4141 tcgatgcgca tcgcccgccg atacaggctg accgcgttgt cggcatcggc ctgtatgacc
     4201 gggcggccgt cgatgacgct gcggacatat cgcgtctcac gcttggttgg cagcacgaag
     4261 ggcttccagg ccacgtcgga ctgtgctggc gcgcgcggag gcggcacgtc gcgcatggat
     4321 tcgcagccag cgacacccag ccccagcacc acggcgacga cgcggaacgc ggcgcgagca
     4381 ccgccccggc gccgaactgc gttcacggca tcagaaacga tgacgcaatc caagcgcgag
     4441 gcttgtcgaa tcacggccag catgcgaagt gacacctccc aggtaaccac cgccgcccgg
     4501 cgcggcctgc cccagcccgg tgttgttgaa ccgggtggcg atggcactga gatcggtgcg
     4561 cttcgacagc gcgtagctgt aggccacgcc aaaggatgcg acatcggcat tggctgcctt
     4621 cgaatcgtca tagagattgc acgcgacgta gaccgtgccg ggtccgctca cgtgcttgta
     4681 gcccaggtga tacagctttg cgtcctgctt gagtgcgtcg gtgtagcccg cctgtaccag
     4741 tgcagccagc gccggaccaa tcagcggtgt gatcggaaca gctatacccg aaacggccga
     4801 cgggtgacca tccctgatcg aggccgccga cgcatagaac tgtcccgagc cgaagttgag
     4861 atatgcgccg aacacgaggc tggtgagcga ctgctgcccc ttctcgttgc gacggttgtt
     4921 gtaggccacg ccaagaccaa aggcatcact gcgatacatg ccgagcaccc cggcaaaacg
     4981 gccggtttcc gtggtcttct cgtcgaagcc gtacatcatg gatgcggtcc atggtccctg
     5041 ttgaagccgg tacgccagcg cattcgaact tcggatgtcg atggcatacg gcaccgcggc
     5101 aatctggcct gaagacaagg acgactgtgc agacaacgca tcgaacgtgc ctgccagttc
     5161 atagcccggc gtgtacatgc ggcctgcgag cacggcaccc accggagtga ccaggcccac
     5221 atagatctgg cgatcgaaga agttggggcc agcggcatgc acattgatac ccacgcgcgt
     5281 gccgattccg gcagacacct ggttcaacaa gggctggagg gctgactgct gcgccgctgg
     5341 cagggaaggc aacaagtggc gcgcgaaggt ggcccggtca ggcaactgcc cgcgcgaggg
     5401 gggtcgattg ctgatgtcgc ccgtgtcggc ctcgagcctg ttctccagcg tgaagatcgc
     5461 tcggtagcca ttcccgaggt cttcgttcgc acgcagaccg attcgcgagc cctccatgat
     5521 tccgctggag agttgcgtca cgctgcctcc cttgaccccg ctgacccggg tcacgccagc
     5581 gtccacgatg ccgtacagct cgagcttgct ttgcgcatgg ccgctcgact gaacggaagc
     5641 caggatgacg aagacaagga ggtggcgttt catgaaggtc tttcgcgaca tgccggctgc
     5701 gctgcggcag tgtgtcatcg accagggcaa gctcgcggac gatgttgcac gagcaacacg
     5761 agcggtttcc ccaaaaagtg aagccgcctt tcgggcggct tcatttcatt agagtaccag
     5821 ctgatcgccg gccttcgatc agaagttgtg gcccagaccg agaacgtact cgttgcgtcg
     5881 ggcaaagttc ctgttcagat caccgctgcg ctcggtgaca gcagcgtaga cgctggttcg
     5941 cttggaaagc gcgtaggccg ccgacaggcc gtacttttct tgatccagat cgctcagagc
     6001 accggtgcgg tccaccgtcg aggaggtgta ctgcgccttg aaagtgaaat tgccgtaggg
     6061 cactttcacg ccgacgttgt aggcgtcgtg gtcggtaccc ttcgcgaacg tgggggcagc
     6121 cagttgcggg ccgaaatcag aggtgttctg gtatgccgcg tagacggttg ccaggccaaa
     6181 gttgtagttc gcaccgacag tcacggcctt gttgtagctg cccgaaacca caccacccga
     6241 aaactcctcg tacgacacgg ccgcattgaa cggcccttgt ccgaagatca atcccacgcc
     6301 gtggtactcg gacgccttcg aagtcaccgg cggggtagcc gagctggtgg acttgaaatc
     6361 ctcgttcagg ccgatggtca ggatgccctg aaatccacca aggactggcg agcgatagga
     6421 aaccccgttg tcgatccggg tgccgaaatt ctggaacaac ggccggttgg atgcgaccac
     6481 ctcttggatg ccgatctcgt tttcgcccgt ggcgtcgttg atgcgaacgt tggtttcgcg
     6541 gctgatcgtc tcgattcgac ccaggcggat gtcgcccagg gtctttgagc caatggcgat
     6601 gtaagcctga cggttgaaga aactggatcc agaaaccgtt gagccaccgc ctgcgcccgt
     6661 gtcagcggcg attgacgact cgaggagaaa gtaggcgcgc agaccatcgc ccagatcttc
     6721 actgccgcgc aggccccagc gtgaattgcc aaagccgctc acaccgccgt cgttgagctt
     6781 ggtaacgcct tttccaagcc gcccaccgga ggaagcggcg gcgttcttgc cgggatcctg
     6841 ataggtgatg ttctggtcaa tgcgaccgta caaagtgacc gacgactgcg ccacagcgac
     6901 ggaggcaaat ccgcccagta cggcgacggc aacaagtgac ttcttcattt ggtcatgctc
     6961 cttgattgac agttaaagaa accccgcacc ggctccaatc gacagcagaa atatcgcgag
     7021 cgaagaggtc ttctttgcaa ggtctgttgc cacgcgaaca gggcgggttc ccgaccgagg
     7081 gatcaagaaa aagccgccga ttggcggctt ttcaggcgca tcagatgcgc cgtcttgcga
     7141 aacatctgat cagaaagtgt gggcaaggcc catcagatac tcgttcttgc gcgcgaacga
     7201 ctccttgacg tcgccaccac gttcggtgat cgcggcgtag accgtggtgc gcttcgaaag
     7261 cgaataggcc accgacgcgc cgtacttgtc ctgatccagg ttgctcagcc cgccggagcg
     7321 gtcgactgtc gacgaggtgt actgtgcctt gaaggtgaag tttccgaacg gcaccttcac
     7381 gcccaggttg tatgcgtcgt gatcggtacc cttagcgaaa gtcgtcgatg ccagctgggc
     7441 tccgaagtcg ctcgtgtcct ggtatgcgcc ataaactgcc gcgacaccga agtcgtagtt
     7501 cgcgcccagt gtgacgacct tgttgtagct tccggaggcc gcgccgccag acagttcttc
     7561 ataggtcaaa gatgcattga gtgggccctg tgaaaaaacc aggcccaggc cgcggtactc
     7621 ggcagccttg gaaactgcac cggtcgttgc attgatgctc ttgaaatctt cgctcaagcc
     7681 gaccgtcagg agaccctgga atccgccaat gacgggcgaa cgataggaaa ttccgttctc
     7741 gacgcgcgaa ccgaagttct ggaacagcgg gcgaccggta gcgaccactt ccgtgatgtt
     7801 caattcgccg tcagccgtga cgtcattgac gcgaacagcc atctcgcgag tcatcgtctc
     7861 gacacggccc agacgaatgt cgccaagcgt cttggaaccg atcgcgacat aagactgccg
     7921 gttgaagaat ccggaacccg acagggtggc gccaccgcct gcgcccgtgt cgccagagat
     7981 cgacgattca aggacgaaat acgctcgcag gccatcaccg aggtcttcgc tgccgcgcat
     8041 accccagcgg gacccgccga tgccgttcac tccgccgtcg ttgagcttgg tcacgccctt
     8101 gccgaggcgg ccgccagatg aggcagcagc gttcttgccc ggatcctgat acgtcacgtt
     8161 ctcgtcgaga cgaccgtaga gagtgaccga tgattgagct gcagcgatgg tgctgaacgt
     8221 gccgaggaca gcaagcgcga tgagtgactt cttcatttgg agactctcct gaacaggaaa
     8281 taaaaatcga aaaaacgaaa aacggaaacc gcttcgacca atcaagcgac agattgacat
     8341 gcagggcacc cggggatcag ggtattccac cagagcgttc ccccccttgc aaaggaatgt
     8401 ccgaacaatc tggcagcggt gttgccgagg caccacaagt tgcctcgctt gatgcctgaa
     8461 ctcatgctcg cagcatgtca ggcccttcgc caaggcttcc caatcccagc ccgtgtaggg
     8521 caaagcgaac ttgttatcga atcgcctcgc cgccacactg tgttgccgct tcatgacaaa
     8581 ttgaagacac ctgtttcact gacaccacag ccaccgacgc ctatgctcga ttccctgatc
     8641 atcaccgccg accaagcgct tcgaaccctc tcgggcggag tgaccgctgc gcgcccgata
     8701 cccaggcacc tcgaatccgg gccgacaagc attgcagagg gcgactcggc tctgtccgaa
     8761 gccgagaaat cactgtcagg cgcactgatg agagtcaacc atgtcggtga ggtttgtgcg
     8821 caggcgctgt accaggccca ggcgatcgga accagtgacc cggcgatgcg ccagctgcta
     8881 gaagcggcag cacgcgacga aaccgaccac ctggcctgga cacaacagcg tctgggcgaa
     8941 ctcggtgcac gggtcagcct cctcaatcca ctctggtacg ccggttcgtt cacgctcggc
     9001 tggctggccg gccgcatggg cgacaaatgg agcctcggtt ttctcgtcga gacggaaaaa
     9061 caggtcgagc aacacctcgc gggacacatg aaccgccttc ctgccaccga ccaacgatca
     9121 cgcgccatcg tgcagcagat gaaactcgat gaggctcggc atgccggcga ggcacagcag
     9181 gcgggtgcag ccgaattgcc ggtcgccgca cgttggggaa tgcgcatcct cgccaaggcc
     9241 atgacccgca cggcccatta cctttgacct cgacaggtca gctggccgcg atctcgtaac
     9301 tggtggtgat atcagcggtc ttgccgagca tgatgctcgc cgaacagtac ttgtcatgcg
     9361 acatctggat ggcacgctct aaggccggag ccgggatgtc ggtgcctgac accctgaaat
     9421 gcatgtgaat cttggtgaag actttcgggt cggtgtcggc gcgctccgag gtgagcctga
     9481 cctggcagcc gctgaccgca tgacgccctc gcttcaatat cagaaccacg tcgtaggcgg
     9541 tacagccagc tgtaccggcc agaacggttt ccatcgggcg cgggcccagg ttgcgcccac
     9601 cgccttcagc tgcaccgtcc atcaccagca catgcccact gccggtctcg gcgacgaaac
     9661 ccatgcccag gccggggacc cagttcactg tgcattccat gactattcct cctgttacac
     9721 ataattattg cagcgcagca aaactgccgc tagacttaca ataactgttg cggattcaaa
     9781 gcccgcaggc tccagttgtc tcctccacct cctcatggtg gattcagccc aaggcatcgc
     9841 tgccttgggc ttttttatgc cttcgccaga ttcccgaatg ccggaccgcg atgcgacaag
     9901 gccgctgcac aagctgcatc gtatgatccg cgcccgacgg ccggaccggc ccgtggtacc
     9961 actcgctccc ggagatcgcc atgtctggcc gaaacaaatc tgaggccgtt ccccgaccca
    10021 ctgaaagcgg ggcgctgggg acatcgatcc gaaccgcgag caagaagagc ggcaagggca
    10081 cgactcgggc gatcccactc agtgtcagcg actacctgca aaagattctg accgcgcgtg
    10141 tctacgacgt cgccctcgag acaccgctcg aactggctcg aagcctgagc aagcgcatcc
    10201 agaaccaggt cttcctcaag cgtgaggact gccagcaggt gttcagcttc aagctgcgcg
    10261 gcgcgtacaa caagatggct cacctgtcgg cagagcagtt gcaacgcggc gtgatctgcg
    10321 cctcggcagg caaccatgcg caaggggtgg cgctcggtgc caaacggctg ggctgcaagg
    10381 cgatgatcgt catgccggtg acgacaccgc gcctgaagat cgacgcggtg aagtcgctgg
    10441 gcgggtcggt ggtgctgcat ggcgagagct attcggacgc ctacgcgcac gcactcgagc
    10501 tcgagcagaa gcatgacctg accttcgtgc accccttcga cgatcccgac gtgattgccg
    10561 gacagggaac gatcggcatg gaaatcctgc gccagcatcc cgacaagatc gacgcggtgt
    10621 tcgtggcgat cggcggcggt ggattgattt ccggggtcgc cgcatacatc aaggcggtgc
    10681 gtcccgaaat caaggtgatc ggcgtccaga tgaatgactc ggacgcgatg atccgttcgg
    10741 ttcaggctgg caaacgcgtc atcctgtccg acgtcggcct gttctccgac ggcaccgccg
    10801 tgaagcaggt cggcgtggag accttccgcc tcacccgtga gctggtcgac gggttcatca
    10861 cggtggacac cgacgccgtc tgcgcggcga tcaaggacgt gttccaggac acccgcagca
    10921 tcctggaacc ggccggcgcg ctggcggtgg cagcggtcaa gcagtacgtc gaaacacacc
    10981 aggtcaaagg ccagacgctg gttgcaatcg cctgcggcgc caacatgaat ttcgaccgcc
    11041 tgcggttcgt tgcagagcgc gccgacgtcg gcgaggagcg cgaagctctc ctcgcagtca
    11101 cgattcccga ggagcgaggc agtttcaagc gcttctgcca ggtcatcggc ccacgctcgg
    11161 tgaccgagtt caactaccgc atctcagacg agcgaaaagc ccatgttttc gtcggtcttg
    11221 cgacaaccca gcgcggagac tcggccactc tcgccgccag cttcaacagc cagggctttc
    11281 aggccatcga cctgacacac gacgagctgg ccaaactgca cctgcggcac atggtcggcg
    11341 gacgcagcga actggccagc gacgagcggc tgtatcgttt cgtcttcccc gagcggcccg
    11401 gggcactgat gaaattcctt tcgagcctgc acccggggtg gaacatcagc ctgttccact
    11461 atcgcaatca gggctcggat tcgggccgga tactggtcgg catgcaggtt ccgcacaagg
    11521 acaagaaggc gtttcgcgat tttctcggca cacttgcgta tccgtgtgtc gacgaaacgg
    11581 acaatccggc gtatcgactt ttcctgcgct gaacctcgat agtccggccg cctgacaccg
    11641 tcgctccaga gaccccaccg agatctgcgc agccatgtcc agctaccaca acctgatttc
    11701 gccgacgggc aacacgcagc tcgaactggc tttgcgcgaa aagctcttgc gccgcagcca
    11761 gaccacgggt gagctgggcg aactcgaacc tctggcggtg cgtctgggat tgatccagcg
    11821 aactctgaag ccgcgcttcc aggcgcctca gatcgtcgtt ttcgcggctg atcacggcat
    11881 cgcggtcgac ggcgttggct cggaggatcg gaattcgact tcggtgctgg tccaggcact
    11941 gctgacatcg aggctgccgt tgtctgtctt cgcacgcatc caggggctgg agttgtcggt
    12001 ggtcgatgcc ggagttgccg aaccagtgag ccctcacccg aggctgctgg cacgcaagat
    12061 cgcgcacggc acgcgcaact cgcgtgtcac ttcggccatg tcactcgaac aggctcatgc
    12121 aggcattcgc gcaggcatgg agatcggcaa ttcgctgccc ggcaatgccg tggtgtgcgc
    12181 cggcatcggt gtgggttcgg ctcaggcatc ggcactggtg ctgtcgcgcc tggccaatgt
    12241 gggcgtgcgc gagttcgcgg tgtcgggccc gagcatgaaa cccaaggacc ttgcgaacat
    12301 gctggcggtg ctgcagggat cgcagaacag gcacaaggaa gtcagcgacc cggtggaggt
    12361 gctggcagcc ttcggcggat tcgagatggc catgatggtc ggcctgatga tggtggccgc
    12421 cggaaagcgg cacctgatcg tcgtcgatgg cttggctgcc tgtgcagcac tggtcgtcgg
    12481 ctcacacatc gcgccgacgg tggtcgacta ctgcgtgttc tcgcgcagtc acgcgcatct
    12541 gggactcgac cgcgcgttgg cgctgttcca ggcccgtgca ctgcttgaac tcggcatgga
    12601 cagcaccgac ggcaccggcg ccacgctcgc atggtcgctg atacgcggcg ccgcggccct
    12661 gctgacagag gtcggcgaag gcgaggaagc gggtccgacg ttgccaggag acatgcccga
    12721 ggtgtcgagg ccccggccga actactgaaa cctaccttac atcagcgcat ggtcactgac
    12781 cggtgcgtcg ggcccggcat gacagcgcca gacgtcggtt cggtgaaggg gagcgccgga
    12841 ggcagcacac ccggtacagc gttggccacc agaggaagtt ccagcacgaa ggcggcagca
    12901 gcgtcacccg cgttgcgcgc cagagccacg gtggccgtgc gcagactgac ctcgcgcagg
    12961 atccattccc cttcgatcac tgccccgacg cgatagaccc gtgccggatt gccatcgaca
    13021 gagatcacgg ccagaccctg ttcgctcgga cgcttggggg ctatcactcc ggtcaacctg
    13081 aacctcgcac tgatatcggg ggcaggagcg tcgaggactt cgtcggttac ctcgacagat
    13141 gccgcaccga agagccgggt caggtcaccc tgagcgccca cctgctcgct gctggcgagt
    13201 gcgagcgcat cagacggtgc agcctgtccg agcagccgca gcccccagaa cacggcgctt
    13261 cctgcgacca gggcccatac gagcagtgcg gacaagcggg acaccatgcg gcgattatga
    13321 tccggcgaca tcgctgcgca ccccggccgc ggcccggatt tccccatgtt cgacaccgat
    13381 tccgatcctg cgttgcaacg ccaccccacc gggcctcatc gatcaacgcg cggcgcactc
    13441 gcctgcggct tcacgctgat cgagctcatg gtggtgctgg tcatcattgg tgttctcgcc
    13501 gcgctgatcg tccccaacgt gctcaaccga gcagacgatg cgcgagtcac tgccgctcgc
    13561 accgacgtca acaacctgat gcaggcgatg aagctctaca agctcgacaa ccagcgcttc
    13621 ccgagtgccg accagggcct gcaggcgctg gtcgccaagc cgaccgtcgg agtgatcccg
    13681 cccaactgga agccctacct cgacaagctg cccaacgacc cgtggggcca tccgtaccag
    13741 tacgccaatc ccggcgtgaa gggcagcatc gacatctaca gcctgggtgc cgatggtgtc
    13801 gtcggtggcg aaggcaacga cgctgaagtt ggctcgtggc agtgagcgga agcggcgggt
    13861 gagatcccgc tgctccggtt tcacgctgat cgagttgatg atcgtcatcg cgctgatcgc
    13921 gctggcggcc tccgtagtca gtctggcact gcgcaacccg gccgacaacc agctcgaaca
    13981 ggagggggcc cgacttgctg cgctgctcga atcggctcgc gcggaagccc gtgcctccgg
    14041 gttggccgtg cagtgggtgc cgcgctcctc cagcggcaac gcaccggcag ccagtggatt
    14101 cacgttcgtc ggcctgcctg catcggcagg cctgccgcaa acttggctgt ttgcgggggt
    14161 caccgccgca gtcatcggag ccccggccgt gctgctcggg cctgaaccga tgatcggcgc
    14221 ccagcgcatc acgctcagcc tcgaggatcg gcggctgacg ctggcgaccg acgggctcgg
    14281 cccgttcatc gtcaccagcg acgaacccgc ggcgccacgt tgatgcagct cattaagctg
    14341 gccggaggcc gcctcttgca ccgcaagcag gcggagcacg gattcacgct gatcgaagtc
    14401 ctggtcgcgc tggtcatcgt cgccatcacc ctgggagccg gaatcaaggc tgcgggtgcc
    14461 ctgctgagca acgcgcaacg actcaccgag gtcagcgaag cgcagtggtg tgcagacaac
    14521 gagctgaccg agttgaagct ggcgaaggca tacccgggca tcggcgagtc gtccttcaac
    14581 tgcgagcaac tgggccgcag ctacgtcggg acattccaga tcagtgcgac tccgaacccg
    14641 aatttccggc ggatcgaggc gcggatcgca aacgaggcag gcgagacgat gctcacgctg
    14701 tccacgatcc ttccgcgcta ttgatgatgg attgccaagc atcgcgccaa agcggtttca
    14761 ccctcgtcga ggtgctggtc gcgctggtcg tcatggcaac cctggcgctg atggcctggc
    14821 agggcgtcga tggcatcgtg cgcacgcgcg acgccagcca gtctcggctg gaggccacgc
    14881 tgcgcctggg aacagtcatc gcgcaatggg aacaggatct ggcctcgatc caggagaccg
    14941 atgtggtgcc ggcgctgact ttcgatggtt ccacactgcg cctgacgcgt cgcaccgatg
    15001 acggcattca gctcgtggcc tggtcgctgc agccttcgac cgggcccgag ggggtgcgca
    15061 accagtggct gcgctgggcc gggccgtccg cgaccagcag cggccccttg caggaaagct
    15121 ggttgcgcag ccagtccctt caaggcaacc cgcagggcgc ctcgcgcacc ttgacaggca
    15181 tctcccaatg gcaggtctac tgctaccggg gcaatgcctg gaccaactgc cagtcgagcg
    15241 gtgacgtggc tgccaccgcc ggcggagccg gacctgcgaa ggtcgtgctc ccgtcgggtc
    15301 tgcgcctggt gctgaccttt tcgggcgggg atggcttcga tggaaccctg acccgagacg
    15361 tggccttcgg cccgcaggcg caatgagatc gaagactttc atcggacgac ctcttcaacg
    15421 acaagcgcag cagggggcag cgctgctgac cgcgatgctg atcgtcgtgc tggtgacgac
    15481 actggcgtcc gcgatggtct ggcagcaatg gcgagcggtg caggtcgagg cggcagagcg
    15541 ggcgcgaacc caggcggcct ggatccttgc cggggcgctc gactgggccc ggctgatcct
    15601 gcgcgaggac gcccgctctg gcagtgccac cacactcaac gacccgtggg ccacgccgct
    15661 ggcggaagcc cggctgtcga gtttcctggc agcggacaag gcccacaccg acgatgggcc
    15721 cgaggccttc ctttccggag cgatcgccga cgcgcaatca cgctacaacc tgcggaacct
    15781 ggtcgacgac accggcaagg tgatccccgc ggaagccgcc gggctgcagc gcctgttcga
    15841 atcggtgggc gtctctccgg atctggcgac acgcatcacc agcggcctgc gcggcgcact
    15901 ggcacccgcg ggcagcgaag accgccccgc gtccccgccg ttgatgccgc gcaccgtatc
    15961 ccagctgaca tggctgggcg tggacgccgc ttcgctgcgc cgcctggctc cgtatgtggt
    16021 gatcctgccg gtccgcactc cggtcaacct gaacacggcg ccacgggagg tcatcgcagc
    16081 cagcgtagaa ggactggacg tcggcgtcgc cgagcgactg gtgcaggctc gcaagcgcac
    16141 gccgttcaag acggtggccg atgcgcaggc tgcgttgccg gacaacctga cactcgacac
    16201 caaccgggtg ggcgtgctgt cgaacttctt cgaagtgcgt ggtcgcgtcc ggctcggcga
    16261 ccgcatgctg gaggatctgt cgctggtcga gcggcgggga ctcgacatcg tgccactgca
    16321 acgccagcgc ctgagcctgc gagacaccgc agggtgaacc acgaggctcg caggctcccg
    16381 ggagcccatc ggcaggcgcg ccaatgcggt aagtcactga tttcacgagg cgcaggcggc
    16441 cccgacgcct aaaatcagag cgtcccgcgc cgtccgcctt tttccttatc ccatgtccat
    16501 cctcgtcatt tcgctggcgc agcgtccacg cattcgagcc cgcagcgcca gcctcgacga
    16561 ggccgtcacg ggcccatcgc acgcagatga cgacatcgaa catgtgttca gtcgcgacgg
    16621 gttctcgatc caggcccaag gacgatgcgc cgcagcttca ctgccgaagg ccgacagcgt
    16681 ggtcgcggtg gtcggcgagg ccgacattgc ctggcaccgc atcaccctgc ccaaggcgcc
    16741 ggcgggtcgg ctgcgcgctg cgctctcggg cgtcctggaa gaagctgtgc ttgccgacat
    16801 ggaggcgact cacttcgccc tcgccccggg ttccgcggca ggccaaccca cctgggtcgc
    16861 tgcaatcgac cgcgcgtggc tgcaggagga gttggctcgc ctcgaacgtc atcaggtctt
    16921 cgtcgatcgg atcgtgccgt tggcctggcc tgacgattcg ccaagtgggc acttcgccga
    16981 ggcgagcggc accggtgagc ccgaggaggc caccctcacc tgggcgcatt caggcggtgt
    17041 cgcgaagctg agattgcagg gcagcctggc gcgttcgctg gtgccgcagc ctgccgaggc
    17101 cggaacgcgc tggaccgcca cgcctgaagt cgccaccgtc gccgaacgct ggctgggtgc
    17161 accggtgctt gcgatctcga agggcgaacg cgcactgcag gccacacgca ccctgtggaa
    17221 cctgcggcag ttcgacctgg ccgcacggca ccgtggcacg cgagcggtgc gcgatctggc
    17281 tcggcagtgg ctgagcccgg cctggcgacc aacgcgtcgg ggcttgctgg tgctggtggc
    17341 cgtacaggtg gtggggctga atctgtgggc gtggcaccag agcgccgaag tggcccgcca
    17401 gaagcaggcc atggtggccg tgctccaggg ctcgttcccg caggtgcgcg cggtactcga
    17461 cgcgccgctg cagatgcagc gcgaagtcga ggcgctgcgg gcgcaggccg gcaaggcttc
    17521 agagaccgat ttcgaaccga tgctgtacgc cgccgcatcg gcctggccgg aaactcagcc
    17581 gcaggtaaac agcatgacct tcgagccagg ccgattgacg ctggccgcac ccggctgggc
    17641 cgacggcgaa atcgaacagt tccgggttca actccgccag gccggatgga acgtggacgc
    17701 agccaacgga caggtcagcc tgagccgact cgctgcgggg ctgaccccat gagtggccca
    17761 gcagaaaaag cattgccggc agcatcgtcg gcggaagcgt cgccgcgacc cggcacgaca
    17821 cctgcctcaa cgcgggtcct gggtcggcaa ctggaagcgc gatgggctgc tctggcaccg
    17881 cgggagcgtg tctggctcaa gggcgccggc gccgtgatcg tggtgttcat gctgtgggtc
    17941 ctggccatac agcccgcatg gcggaccgcg cgagcggcac ctgcgcagct cgatcggctc
    18001 ggcatccagt tgcagacgat gcagcagctg ggcgctgaag caaccgattt gcgcaacagc
    18061 gctcccgtgt cggccgcaca ggccggtacc gcccttcaag ccgctaccga acggctgggc
    18121 tcgagggcac aactgctgat gcagggcgag cgggtcaccg tcaacgtgaa gggcttgtcg
    18181 ggaaacgacc tgatcgactg gctggccgag gcccgcagcg gcgcgcgtgc gagggccacg
    18241 gaagttcggc tggcgcgtga cgcacagggt tacagcggca ccgtggtcct cagcctgggc
    18301 agtggcagat gaggcgagcg tcgagcagcc gctggtccga atcgacgcac gctgaactcg
    18361 cgtggggacg ttcacgcgcg agctccgtgc attgggcctg ggctggcgcg ctgatcggga
    18421 tactgatctc gtccatatgt tttgttccag ccagctggct ggctggcgtg atatccgcgg
    18481 cgacccaaca aaggttcatg ctggctgacg cacgcggcac cgtctggtcg ggcagcgcaa
    18541 cggcggtgct gacgggcggg ccgggcagcc gcgattccag cgcccttccc ggccgactgt
    18601 tctggtccgt gcaaccgcag gggctggcgc tgaaggtcac cctgagccag gcctgctgca
    18661 tcgagggccg tgtgggcgta cttctgaaac ctgggtggag cggcttcagc gcaatgctgc
    18721 agcccgagac ggatcgaatc ggccaatggc ccagccgatg gttgaccgga ctgggcactc
    18781 cgtggaacac gctgaaactc ggcggcacca tctacttgca gtcgtccggt ctgacactcg
    18841 aatcgacgaa cggacattgg cggatctcgg gcggaatgga tctcgaattg atcgacgcat
    18901 cctcgagcct gtccacactc gagccgctcg gcagctaccg cctggcgctg cagggcacgc
    18961 cacccagcga agcgcccgac ccccagaccg gatcgacgga gtcggctctc ctcacgctgt
    19021 cgacgctcga aggggctctg ctgctcagtg gcaacggaac ctggggcgcc aaaggcctga
    19081 actttcgcgg cgaagcgacc gcgcgcgacg caggcgatga ggcgctcacc aacctgctga
    19141 acatcatcgg gcggcgcgac ggcgcgcggt ccgtcatttc gatcggatag ccatgaacgt
    19201 tcatcctccc gtgtcttcgc tgtcgcgatg gctgcgccga cacgctacgc ggctcggcgc
    19261 ggggacggca tccctgccgc tgacgctttc gctggtggca accccgttag gtactctgcc
    19321 tgcgccggcg cgcgcccagg cggcggccgg cgaagcggtg gccaaccgct ttcgcggcga
    19381 gcccgtgacg ctgaatttcg tgaacgccga catcgatgca gtctcgcgcg cgatcgctgc
    19441 gatcctccgc cagcagttca tcgtggatcc gcgagtcaag ggcaccatga cgctcttcag
    19501 cgaacaggcc ctgccgccgc gcgaggacta c
//
LOCUS       DAOORV010000066        19185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3475_length_19185_cov_7.498693, whole genome shotgun sequence.
ACCESSION   DAOORV010000066 DAOORV010000000
VERSION     DAOORV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 19185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_19185_cov_7.498693"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKV56_10245"
     CDS             complement(<1..676)
                     /locus_tag="PKV56_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430765.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOX68235.1"
                     /translation="MRVLLIHPNYHSGGAEIAGNWPPAWVAYLTGYLKAGGYTDVVFV
                     DAMTHHLSDDQVRQRILEIQPDIVGCTAITPAIYKAERLLQIVKEVDPDIVTVLGGIH
                     GTFMYPQVLKEAPWIDAVVRGEGEQVFLNLVTAVDDGSWRIDRRQVNGIAFAEDGKVV
                     ATAAEPTIQDLDRILPDWGILEWDKYQYIPMGVRVAIPNFARGCPFTCTFCSQWKFWR
                     DYRIRDP"
     gene            complement(814..1725)
                     /locus_tag="PKV56_10250"
     CDS             complement(814..1725)
                     /locus_tag="PKV56_10250"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOX68236.1"
                     /translation="MKKLLFLGAGGVGGYFGARLLEAGVDVSFLVRPARAALLAGQGL
                     RVSSPHGDLALPVRCVTRDSVQPEYDLVLLAPKAYDLEDALDSIEPAVGPGTFVMPFL
                     NGLAHMEALDARFGAHRVLGGVAHIAAALAADGTVRQLSPVHVMTAGGRAPATQQAAA
                     DFIALCGPANFDAVLSSDIIATLWEKWTFLATLAGATTLMQGSIGQIMDTVHGESLLR
                     RLYAEGLAVADRSGFPVPEKAQAKSLRVLTERGSGFTASMLRDLQAGQRTEHDHVLGD
                     LVKRARVHGVDTPLLEAAHCHLQVAAK"
     assembly_gap    1795..1894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1895..3259)
                     /locus_tag="PKV56_10255"
     CDS             complement(1895..3259)
                     /locus_tag="PKV56_10255"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOX68237.1"
                     /translation="MTHSPAFPKRIQTEESATFDRTALDYIHNAAAHGLYEIRGLGAK
                     RKLPHFDDLVFLGASLSRYPLEGYREKCLTKTVLGTRYATRPITLDIPITIAGMSFGS
                     LSAHVKEALGLAATAMGTSTTTGDGGMTPEERNSSKTLVYQCLPSRYGFKPDDVRRAD
                     AIEIVIGQGAKPGGGGMLLGQKVNPRVAAMRTLPEGIDQRSASRHPDWTGPDDLVIKI
                     HELREITDWKTPIYVKIGATRVFNDVKLAVHAGADVVVLDGMQGGTAATQTCFIENVG
                     IPTLAALRQAVDALEDLNMKGQVQLIVSGGIRTGADVAKALAMGADAVSIGQGVLMAL
                     GCNHDSYEQDGQRIDATADYAALGARPGSCHHCHTGRCPIGITTQDAVLEQRLQPASG
                     ARHLKNYLKTLTMELTTIARACGKQNVHHLEPEDLVALTVEAAAMARLPLAGTNWIPG
                     VGAI"
     gene            complement(3267..3962)
                     /locus_tag="PKV56_10260"
     CDS             complement(3267..3962)
                     /locus_tag="PKV56_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HOX68238.1"
                     /translation="MEMTTFDLATQTVRELNQHLHQPAGELAGREVVVANPNGAHNLA
                     VGLNAPVHVTVQGHAGYYAAGMNQLATVVIEGSAGTGVAENMMSGRVHVKGFASNAAG
                     ATAHGGLLVIEGDAGLRCGISLKGGDIVVGGSVGSFSAFMAQAGRMVICGDAADALGD
                     SIYEAVLYVKGEIKSLGADAQIEPMTSADIATVAELLKSAGLKTAHDPAAFRRIASAR
                     TLYHWNADADQDY"
     gene            complement(3962..4873)
                     /locus_tag="PKV56_10265"
     CDS             complement(3962..4873)
                     /locus_tag="PKV56_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOX68239.1"
                     /translation="MCGIVGLLVKKPALKDQLGALMVPMLLGMTERGPDSAGLAVFGE
                     ALDAGHRKLSLYSGLTAEGSQFGWAALVTALNADMPAARASAHATGNHAVLTVAVDPT
                     PVKDWLHEQHPKLHILSTGRSIDLYKDIGTPGEVAARYDFAHLRGTHLVGHTRMATES
                     AVTPDRAHPFTAGEDFCLVHNGSLSNPYGVRRKLEPLGIHFDTDNDTEAACRFIEWRL
                     RDGDDLPTALQRSFDELDGFYTLLMGTADQLALIRDPFACKPAMVAETDDYVAIASEF
                     RALAHLPGINHANVFEPAPEEMYVWKA"
     gene            complement(4901..6280)
                     /gene="glnT"
                     /locus_tag="PKV56_10270"
     CDS             complement(4901..6280)
                     /gene="glnT"
                     /locus_tag="PKV56_10270"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HOX68240.1"
                     /translation="MSTTPKPSDRVAPLRFQALTDATSYLDAQGVKYVLAQFVDIHGT
                     AKAKAVPVAHLPSVLGDGAGFAGFAIWGVGIEPHGPDFMAVGELGTLSLVPWQPGMAR
                     IVCDGHVHGEPWAFDSRVTLKKQIERLTARGWTLNTGLEPEFSLLRRSVTGAIEPCDA
                     SDTLAKPCYDYKGLSRTRAYLEKLTESLHAVGIDVYQIDHEDANGQFEINYTYTDCLT
                     SCDHYVFFKMAASEIANEMGMVCSFMPKPFAHLPGNGMHMHASIADAAGANLFEDKSD
                     PQGLNLSMMAYHFLGGLLAHAPALTALACPTVNSYKRLVVGRSLTGATWAPAYISYGD
                     NNRSTMVRIPKGRLELRLPDGSCNPYLATAAVIAAGLDGIERQLHPGPAHNENLYDLT
                     PAQLRERGIAVLPQNLDRALDALEADTLIVEALGPVACEFIRLKRMEWIEYMRHVSDW
                     EIKSYLEFF"
     gene            6355..6978
                     /locus_tag="PKV56_10275"
     CDS             6355..6978
                     /locus_tag="PKV56_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOX68241.1"
                     /translation="MKKHTPDTVPPPVEHPPSLEKSLGSAIRELRQRHQLTLAQVSEQ
                     ASVSRSMLSKIETGQIMAGLDTLSRIARALGVSMSMLFRNYDVPSGAAQLVKRDAGMQ
                     VVRRGTKSGHTYKLLAYDQGPVKRFEPFLIEMNEQSESFPTFEHPGTEFIHMLEGTME
                     YRHGNTVYVLEPGDSLTFAGEILHGPERLLVKPIRFLSVILYPGAPQ"
     gene            7047..7451
                     /locus_tag="PKV56_10280"
     CDS             7047..7451
                     /locus_tag="PKV56_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68242.1"
                     /translation="MSRFHAAAAASLLLSFVSLQPVQAAEVGEQWEYSGTMDMMGMKM
                     PMPPSKVCSRPGAESTPTADKRCKVSDVKTTASKTSYRVSCPPPDAMEGSGESERKGD
                     TVVSSFRMKSKDGEMKMSMTGRKLGPCTPSAN"
     gene            7467..7934
                     /locus_tag="PKV56_10285"
     CDS             7467..7934
                     /locus_tag="PKV56_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOX68243.1"
                     /translation="MLRTLKDLFDAVMPPRAGDTPDHTEHAMHLAAAVLLVEVMRSDA
                     EISQDERSAVLSALQARFNLSDDEGARLLELAHQAAHGATDYFQFTSKINDSFDMDQK
                     IVLVEQMWQVAYADGVLTAHENHVMRRLSDLLHVPHGAYVSAKMRARQAAGAA"
     gene            complement(7959..9371)
                     /locus_tag="PKV56_10290"
     CDS             complement(7959..9371)
                     /locus_tag="PKV56_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HOX68244.1"
                     /translation="MNRLNQQLLRVWAGLGSRFLPFADAATPDLPLSKLLRLSLFQVS
                     VGMAMVLLVGTLNRVMIVELGVPASLVAVMIAMPLVFAPFRALIGFRSDTHASALGWR
                     RVPYLWKGTMLQFGGLAMMPFALLVLSGGGNASQWPAWFGQLGAALAFLLVGAGLHMT
                     QTVGLALATDLAPVESQPKVVGLMYVMLLMGMIVSSLVFGALLSDFTPAKLIQVIQGA
                     AVVTIVLNIAAMWKQEKRNPSRNSGTVKEQSFRDSWDSFCQGGNAVRRLCAVGLGTMA
                     FSMNDILLEPYGGQVLQLSVAATTKLTATLAVGGLLGFGLASRVLSRGADPFRMAGYG
                     ALAGIPAFLAVIFAAPADSPILFAFGTMLIGFGAGLFGHGTLTATMNLAPKNQTGLAL
                     GAWGAVQASAAGLAVAMGGIIRDVVASMAEQDRLGHALNVPATGYIFVYGIEVVMLCA
                     TLIALAPLVRRIAPVAALSR"
     gene            complement(9445..9678)
                     /locus_tag="PKV56_10295"
     CDS             complement(9445..9678)
                     /locus_tag="PKV56_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0045156 - electron transporter,
                     transferring electrons within the cyclic electron
                     transport pathway of photosynthesis activity [Evidence
                     IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting protein"
                     /protein_id="HOX68245.1"
                     /translation="MNQGKVWRVVNPTIGVPIFLGSVMVGSLIVHGGLMINTDWFGAY
                     WNGGQKAAAAAAAKPAAALAPDTIVSQAPTPQK"
     gene            complement(9691..9846)
                     /gene="pufB"
                     /locus_tag="PKV56_10300"
     CDS             complement(9691..9846)
                     /gene="pufB"
                     /locus_tag="PKV56_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HOX68246.1"
                     /translation="MADDPNRVWPSGLTTAEAEELQRGLVEGTRVFFFIAILAHVLAY
                     AYTPWLH"
     gene            complement(10048..10599)
                     /locus_tag="PKV56_10305"
     CDS             complement(10048..10599)
                     /locus_tag="PKV56_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68247.1"
                     /translation="MSTHFNLKTTLCIAALLALPVAQAASMAEADYKAAKIRINSEYK
                     AQKTGCGSLAGNALDICEEEAKGNEKVALAELEYNYTGKPADHNKLMKAKAEAAYEVA
                     KEKCDDMAGNAKDVCVKQAKAVEAKALADAKLDKKVDEARSDASQSKTDADYKVAAEK
                     CDALAGDAKAGCLTAAKARFGKT"
     gene            complement(10748..11155)
                     /locus_tag="PKV56_10310"
     CDS             complement(10748..11155)
                     /locus_tag="PKV56_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOX68248.1"
                     /translation="MSRYLPAFAAILLVMIALDVLWLGFIAKPMYQNGIGHLMAERPN
                     LWAAGAFYVLYASGLTVFAVMPHVGDSGWSGTASRAALFGLVAYATYDLSNLATLRSW
                     PIGLALIDMAWGSALSAMSAVAGRAAHGWSASG"
     gene            complement(11152..12483)
                     /locus_tag="PKV56_10315"
     CDS             complement(11152..12483)
                     /locus_tag="PKV56_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HOX68249.1"
                     /translation="MSLNPILVICAVLSLAACGDVAKLPLSAGVGPHPTLPAPRPALI
                     PTVNIAPAVGWPEGLTPQAAPGTQVSAFARDLDHPRWLLVLPNGDVLVAESNAPPRPS
                     DGQGIKGWVAGWVMKRAGAGVPSADRITLLRDTDGDGVADLRSVLLEGLRSPFGMALV
                     GDTLYVANSDSVVKFPYTPDTTRITAGATHLVDLPGGAINHHWTKNLIASVDGSKLYV
                     TVGSNSNVGEHGMDAEAQRAAIWEVDAASGAHRIYASGLRNPNGLAWTGARGDGSSAP
                     VLWVVVNERDEIGSDLVPDYLTSVRDGAFYGWPYSYYGQNVDVRVQPPQPEMVARAVS
                     PDYALGPHTASLGLASSAGTSLPAPFAHGMFVGQHGSWNREPHSGYQVVFIPFDGDKP
                     SGAAPVEVLTGFLSRQGEAHGRPVGVALDRKGALLVADDVGNVVWRVSAKP"
     gene            complement(12543..12701)
                     /locus_tag="PKV56_10320"
     CDS             complement(12543..12701)
                     /locus_tag="PKV56_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HOX68250.1"
                     /translation="MSVGTILLIILILLLIGAFPSWGHSRSWGYGPSGGLGLIVIIVL
                     VLVLTGRL"
     gene            complement(12736..12978)
                     /locus_tag="PKV56_10325"
     CDS             complement(12736..12978)
                     /locus_tag="PKV56_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68251.1"
                     /translation="MNHLKLASALLVAASLAACDKPTVVNVPPPAVGVPGPAGPQGES
                     GATGNQGADGNKGATGSTGDGSTVIVLPPAASAPAN"
     gene            complement(13153..14565)
                     /gene="fumC"
                     /locus_tag="PKV56_10330"
     CDS             complement(13153..14565)
                     /gene="fumC"
                     /locus_tag="PKV56_10330"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOX68252.1"
                     /translation="MNSTRIESDSFGPIEVPADALWGAQTERSLRFFAIGEQRMPLAL
                     IHAVAWVKWAAACVNRDLGLLGESRAQAIADAALRVAAGEFDAHFPLSVWQTGSGTQT
                     HMNVNEVVAHLASQALAGGEVGGVDAAATAARAVHPNDDVNLGQSSNDVFPTAMHLAV
                     ALRARDRLLPALAELRAALAAKAIAFQFVVKIGRTHLQDATPVTLGQEFGGYEAQLAL
                     CQQALRHALVAVHALAVGGTAVGTGLNTHPEFGSRVAALLARELDLPLVQADNLFAAM
                     AGHEALVAFHAALKMLAIALTKIGNDIRLMGSGPRAGLGELRLPENEPGSSIMPGKVN
                     PTQVEALTMVCAQVMGHDAAIGFAASQGQFELNVYKPLIALNTLDSLRLLTDAMNSFD
                     RHCVAGLSANSARVEQMLQGSLMLVTGLAPHIGYDRAAQIARSAHDHGTSLRDAALAS
                     GAVSAEQFDGWVDPRRMLGA"
     gene            complement(14576..16549)
                     /locus_tag="PKV56_10335"
     CDS             complement(14576..16549)
                     /locus_tag="PKV56_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOX68253.1"
                     /translation="MLGGLMCLLAAGEGLEWPFLAAPLEKSLSSATSRRVTLSQAADT
                     QAAASRRFGVRFVGGVRLHTPLLVIGAPAWSGQPHLLLARDVELRLRYIDLWRAYRGQ
                     TLRIESLQASVLDAQLERLADGRATWQLSAEPAGLPKQPVSIPSFGRLQVSDGVLSYR
                     DTPLAIDVEARLSLVASDLKLSAAGRYRALPLKIDLLASDVLAPTTGELRTVSSPVVL
                     DATVGRARVTFDGVVDDALDFNGFSGRFSLKGPSLAAVGDPVGVTLPTTAAFAARGVV
                     VRKGGEWQVRLDDVTVGSSRLNGAFGYRVGDAVPLLSGRLGGSRLQLADLGPVVGTTA
                     AAPMAAASSPLPVAEVAGKVAGKVLPVRPFDLAALRAMDANVLIDIAEVDLNTTLLEP
                     LRPLRAHLQLTAGVLVLSGLDARTADGRLKGRWSLDGRGSEAMWDADLRWTDVRLERW
                     IRQARTGGAPPYVSGRLGGRATLKGQGRSTAAILASLDGRVRGELSGGSVSHLVVEAA
                     GLDVAQALGVLFKNDDALPLGCAVADLNAVRGVFSPRMMVLDTTDSAVWIDGSLSLAA
                     ESLDLRAVVSPKDFSPLTLRSPLRVRGTFADPEFTLERGPLSGKVASALLLALLTPLA
                     AWIPLIDTGDADLAQRQGDACRSLMRRHAAPKS"
     gene            complement(16629..17126)
                     /locus_tag="PKV56_10340"
     CDS             complement(16629..17126)
                     /locus_tag="PKV56_10340"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOX68254.1"
                     /translation="MKIQSQSSAELIHLAQLIQPMDTVAMLTSHDADGALVSRPMAPL
                     EMDALGALWFFTHRRSAKIEQLKALNLCFVDAERGTYVSMSGHGDIVDDREHVKRLWS
                     PLARPWFPHGPDSEELTLLKFTPHSAEYWDAVDTKMVRLFATAVSVVAAKPIGLGEHG
                     HLAHL"
     gene            complement(17558..17980)
                     /locus_tag="PKV56_10345"
     CDS             complement(17558..17980)
                     /locus_tag="PKV56_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX68255.1"
                     /translation="MKIVFAADGSKCTKKALAFLVTHEGLLGEQGELFVLNVQAKLPP
                     HVRRVLGSAAITEYASDEADKVLVPVRRFLDRHPVRYRTAWVVGTPGEEIVKAAKREE
                     AHLIVMGTHGLGLIGRALMGSVAQRVITDCDIPVLLVK"
     gene            complement(18024..18479)
                     /locus_tag="PKV56_10350"
     CDS             complement(18024..18479)
                     /locus_tag="PKV56_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68256.1"
                     /translation="MTSIRNLALAAVATALCLAGPAARAAADHSSHQPAASASAPGDA
                     QGAAAEASRMDEHMKHMREMHEKMMNAKTPEERSALRAEHMKAMQEGMAMMGGAGAGG
                     MQCHGAKQGKGSMHDNMAAHHEAIEKRLDMMQSMMQMMLDRLPATPAKQ"
     gene            complement(18671..18871)
                     /locus_tag="PKV56_10355"
     CDS             complement(18671..18871)
                     /locus_tag="PKV56_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOX68257.1"
                     /translation="MIAYEVKDMTCGHCVNAITQALKAVDSTAEVRIDLAQHRVEIES
                     SEAGAASLGDAIRDAGYTPVAV"
     gene            18958..>19185
                     /locus_tag="PKV56_10360"
     CDS             18958..>19185
                     /locus_tag="PKV56_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOX68258.1"
                     /translation="MNTTASAKEQGSALQLQITGMTCASCALRVEKALAKVPGVLQAS
                     VNLATEQACVRADASVGTQVVADAVHRAGYGV"
BASE COUNT         3177 a         6441 c         6388 g         3079 t
ORIGIN      
        1 tcgggtcgcg gatgcggtag tcgcgccaga acttccactg gctgcagaag gtgcacgtga
       61 acggacagcc gcgggcgaag ttcggaatcg ccacccgcac gcccatcggg atgtactggt
      121 acttgtccca ctcgaggatg ccccagtcgg gcaggatgcg atcgaggtcc tggatggtgg
      181 gctcggcggc cgtggcgacc accttgccgt cttcggcaaa ggcgatgccg ttgacctggc
      241 ggcggtcgat cctccagctg ccgtcgtcca ccgccgtcac gaggttcagg aacacctgct
      301 cgccttcgcc gcgcacgacc gcgtcgatcc acggtgcctc cttcagcacc tgcgggtaca
      361 tgaaggttcc gtggatgccg ccgagcaccg tgacgatgtc ggggtcgacc tccttgacga
      421 tctgcagcag ccgctcggcc ttgtagatgg ccggcgtgat ggcggtgcag ccgacgatgt
      481 cgggctggat ctcgaggatc cgctgccgca cctggtcgtc gctcaggtgg tgggtcatgg
      541 cgtcgacgaa aacgacgtcg gtgtacccac cggccttcag gtagccggtc aggtaggcga
      601 cccaggcggg tggccagttg ccggcgatct ctgcgccgcc ggagtggtag ttcgggtgga
      661 tcagcaggac acgcatcggc gaggctccat cggtacgggg ttccgcacaa taccgatgca
      721 gccaggccaa cgaattgcgc tatgtcaaca aatgttgaca gtcttcatta gcgttttcat
      781 tgacgcccgc aggcgcgctc gcgtagccac gggctacttc gcggccacct gcaaatggca
      841 atgggcggct tccagcagcg gcgtgtcgac accgtgcacc cgagcccgct tcaccagatc
      901 gcccagcacg tggtcgtgtt cggtgcgctg tcccgcctgc aggtcacgca gcatcgacgc
      961 ggtgaatccc gatccgcgtt cggtcaggac gcgcagggac ttggcctggg ccttctccgg
     1021 gacggggaac ccgcttcgat cggccaccgc cagaccttcc gcatacagcc ggcgcagcag
     1081 cgattcgccg tgcacggtgt ccatgatctg gccgatcgag ccctgcatca gcgtggtggc
     1141 gccggccagc gtggcaagga aggtccactt ctcccacagc gtcgcaatga tgtcgctcga
     1201 caggacggcg tcgaagttgg ccggcccgca cagcgcaatg aaatcggccg cggcctgctg
     1261 tgtcgcgggg gcgcgcccac ccgcggtcat cacgtggaca gggctcagct gccgcaccgt
     1321 gccatcggca gccagcgccg ccgcgatgtg cgccacgccg cccagcacgc ggtgcgcacc
     1381 gaagcgtgcg tcgagcgctt ccatgtgtgc caggccgttg agaaaaggca tcacgaaggt
     1441 tccgggcccg acggccggct cgatcgagtc gagcgcgtct tcgaggtcgt aggccttcgg
     1501 cgccagcagc accagatcgt attcgggctg cacggagtcc cgcgtcacgc aacgcaccgg
     1561 cagcgccaga tcgccgtgcg ggctgctcac ccgcaggccc tgcccggcga gcagcgccgc
     1621 gcgggcggga cgcaccagaa agctgacgtc gacaccggcc tcgagcaggc gagccccaaa
     1681 gtaacccccc acgccacccg cgcccaggaa aagcagtttc ttcatcgatc gctccttcag
     1741 gggatcgatg ttggcacggc ccggacaggc cggcgcgcct gcgaccggga tcggnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcagat cgcgccgact ccgggaatcc
     1921 agttggtgcc ggccagcggc agacgcgcca tcgcggcggc ctcgaccgtc agcgcgacca
     1981 gatcttcggg ctcgaggtga tgcacgttct gcttgccgca ggcgcgcgcg atggtggtga
     2041 gttccatcgt cagcgtcttc aggtagttct tcaggtgccg cgcaccggac gcgggctgca
     2101 ggcgctgctc gagcaccgca tcctgcgtcg tgatgccgat cggacagcgc ccggtgtggc
     2161 agtggtgaca cgagcccggt cgcgcgccga gcgcggcgta gtcggcggtt gcgtcgatgc
     2221 gctggccgtc ctgttcgtag ctgtcgtggt tgcagccgag cgccatcagc acgccctgcc
     2281 cgatcgagac cgcgtcggcg cccatcgcca gcgccttggc gacatcggcg ccggtgcgga
     2341 tgcccccgga gacgatcagc tgcacctggc ccttcatgtt caggtcctcg agcgcatcga
     2401 cggcctgccg cagcgccgcc agcgtcggga tgccgacgtt ctcgatgaag caggtctgcg
     2461 tggccgcggt accgccctgc atgccgtcga gcacgaccac gtcggcaccg gcatgcaccg
     2521 ccagcttgac gtcgttgaac acgcgggtcg cgccgatctt cacgtagatc ggggtcttcc
     2581 agtcggtgat ctcgcgcagt tcgtggatct tgatgacgag gtcgtcaggc ccggtccagt
     2641 cggggtggcg gctggccgag cgctggtcga tgccctcggg cagcgtgcgc atggcggcca
     2701 cacgcgggtt gaccttctgc cccagcagca tgccaccgcc gcccggcttg gcaccctggc
     2761 cgatgacgat ctcgatggcg tcggcgcggc gcacgtcgtc gggcttgaat ccgtagcgcg
     2821 acggcaggca ttgatagacc agcgtcttcg acgaattgcg ctcttccggc gtcatgccgc
     2881 cgtcgccggt ggtggtcgag gtgcccatcg ccgtggccgc gagcccgagc gcctccttca
     2941 catgggccga cagcgagccg aagctcatgc cggcgatggt gatcgggatg tcgagcgtga
     3001 tcggacgggt ggcgtagcgc gtaccgagca cggtcttcgt caggcacttc tcgcggtaac
     3061 cctcgagcgg gtagcgagac agcgatgcac ccaggaagac gaggtcgtcg aagtggggca
     3121 gcttgcgctt ggcgccgagg ccgcggatct cgtagagccc gtgcgccgcc gcgttgtgga
     3181 tgtagtcgag cgccgtgcgg tcgaaggtgg cggactcttc ggtctggatg cgcttcggga
     3241 atgcgggtga gtgggtcata gcgggttcag tagtcctggt cggcgtcggc gttccagtgg
     3301 tagagcgtgc gggccgaggc gatgcgcctg aacgcagccg ggtcgtgcgc ggtcttcagg
     3361 ccggctgact tcagcagctc ggccacggtg gcgatgtcgg ccgaggtcat cggctcgatc
     3421 tgcgcgtctg cgccgagcga cttgatctcg cccttgacgt acagcacggc ttcgtagatc
     3481 gagtcgccga gcgcgtcggc cgcatcgccg cagatcacca tgcggccggc ctgcgccatg
     3541 aaggccgaga agctgccgac cgatcccccg acgacgatgt cgccaccctt cagcgagatg
     3601 ccgcagcgca ggccggcgtc tccctcgatc accagcaggc cgccgtgcgc ggtggcgccg
     3661 gccgcgttcg acgcgaagcc cttgacgtgg acacggccgc tcatcatgtt ctcggcgaca
     3721 ccggtgccgg cgctgccttc gatgacgacg gtggcgagct ggttcatgcc cgccgcgtag
     3781 tagccggcgt ggccctggac cgtgacatgg accggcgcat tcaggccgac ggccaggttg
     3841 tgggcgccgt tcggattcgc gacgacgacc tcgcggcctg cgagttcgcc ggccggctgg
     3901 tgcaggtgct ggttcaactc gcgcaccgtc tgcgtcgcga ggtcgaaagt ggtcatctcg
     3961 atcacgcctt ccatacgtac atctcctcgg gggcgggctc gaacacgttc gcgtgattga
     4021 tgccgggcag gtgagccagc gcacggaact cgctggcgat cgccacgtag tcgtcggtct
     4081 cggccaccat cgccggcttg cacgcgaaag ggtcgcggat cagcgcgagc tggtcggcgg
     4141 tgcccatcag caaggtgtag aagccgtcca gttcgtcgaa gctgcgctgc agcgcggtcg
     4201 gcaggtcgtc gccgtcacgc aggcgccact cgatgaagcg acaggccgcc tcggtgtcgt
     4261 tgtcggtgtc gaagtggatg cccaggggct cgagcttgcg acgcacgccg taggggttcg
     4321 acagcgaacc gttgtggacc aggcagaagt cctcgccggc ggtgaacggg tgcgcgcgat
     4381 cgggcgtgac cgccgactcg gtcgccatcc gggtgtggcc gaccaggtgc gtgccgcgca
     4441 ggtgcgcgaa gtcgtaacgc gccgccacct cgccgggcgt gccgatgtcc ttgtacaggt
     4501 cgatcgatcg accggtcgac aggatgtgca gtttcggatg ctgctcgtgc agccagtcct
     4561 tgaccggggt cgggtcgacg gccacggtca gcaccgcgtg gttgccggtg gcatgcgcac
     4621 tcgcccgagc cgccggcatg tcggcgttca aggcggtcac cagcgcggcc cacccgaact
     4681 gcgaaccttc tgcggtcagg cccgaataca ggctcagttt ccggtggccg gcgtcgagtg
     4741 cctcaccgaa caccgcgagg ccggccgaat cgggcccgcg ctcggtcatg ccgagcagca
     4801 tcggcaccat cagcgcgcca agttgatcct tcagtgccgg cttcttgacc agcagtccca
     4861 cgatgccgca catgtgttgg cttccgtttc aatccaggtt tcagaaaaac tcgaggtagc
     4921 tcttgatctc ccagtcggag acgtggcgca tgtactcgat ccattccatg cgcttgagcc
     4981 ggatgaactc gcacgcgacc ggcccgagcg cttcgacgat cagcgtgtcg gcctccagcg
     5041 cgtcgagcgc gcggtccagg ttctgcggca gcacggcaat gccgcgctcg cgcagctggg
     5101 ccggcgtcag gtcgtacagg ttctcgttgt gcgccgggcc agggtgcagt tggcgttcga
     5161 tgccgtcgag gccggcggcg atgaccgcgg cggtcgcgag gtacgggttg cacgagccgt
     5221 cgggcaggcg cagctcgagc cggcccttcg ggatgcgcac catcgtcgag cggttgttgt
     5281 cgccgtagct gatgtaggcc ggcgcccagg ttgcaccggt cagcgatcgg ccgaccacga
     5341 ggcgcttgta cgagttgacc gtcgggcagg ccagcgccgt cagtgcgggt gcgtgcgcga
     5401 gcagcccacc gaggaagtga tacgccatca tcgacaggtt caggccctgc gggtcgctct
     5461 tgtcttcgaa caggttcgct ccggctgcat cggcgatcga cgcatgcatg tgcatgccgt
     5521 tgcccggcag gtgcgcaaac ggcttgggca tgaatgagca gaccatgccc atctcgttgg
     5581 cgatctccga cgccgccatc ttgaagaaga cgtagtgatc gcacgacgtc aggcagtcgg
     5641 tgtaggtgta gttgatctcg aactgtccgt tcgcgtcctc gtggtcgatc tggtagacgt
     5701 cgatgccgac cgcgtgcagc gactcggtca gcttctccag gtaggcacgg gtgcgcgaca
     5761 gacccttgta gtcgtagcag ggcttggcca gcgtatcgct ggcatcgcag ggttcgatcg
     5821 cgccggtgac gctgcgcctg agcagcgaga actcgggctc caggccggtg ttcagcgtcc
     5881 agccacgcgc ggtgaggcgc tcgatctgct tcttcagcgt cacgcgggag tcgaaggccc
     5941 agggctcgcc atggacatgg ccgtcgcaca cgatgcgcgc catgcccggc tgccagggca
     6001 ccagcgacag cgtgcccagt tcgccgacgg ccatgaagtc cggaccatgc ggctcaatgc
     6061 cgacccccca gatcgcgaag ccggcgaaac ccgccccgtc accgagcacc gaaggcaggt
     6121 gggccaccgg caccgccttg gccttggcgg tcccgtggat gtcgacgaac tgcgcgagca
     6181 cgtacttgac gccctgcgcg tcgaggtaac tggtcgcgtc tgtcagggcc tggaagcgca
     6241 gcggcgcaac gcgatcggaa ggcttcgggg tggtgctcat gtcggtctcc gggtgtcgtg
     6301 acaccgccgg ggcagtgtag cgtgatagat tcaccagaag caattttgat tcccgtgaag
     6361 aaacacactc ccgataccgt ccccccgccc gtcgagcacc cgccgtcgct ggagaagtcc
     6421 ctgggcagcg cgatccgcga gctgcgccag cgccatcaac tgacgctggc acaggtgtcc
     6481 gagcaggcca gtgtcagtcg cagcatgctg tcgaagatcg agaccggcca gatcatggcg
     6541 ggcctcgaca cgttgagccg catcgcacgg gcgctgggcg tgtcgatgtc gatgctgttc
     6601 cgcaactacg acgtgccgtc gggtgcggcg cagctcgtca agcgcgatgc aggcatgcag
     6661 gtggtgcgtc gaggcaccaa gagcggacac acctacaagc tgctggccta tgaccaggga
     6721 ccggtgaaac gcttcgagcc cttcctgatc gagatgaacg agcagtcgga aagctttccg
     6781 accttcgagc atccgggcac cgaattcatc cacatgctcg aagggacgat ggaatatcgg
     6841 cacggcaaca cggtctacgt gctggaaccc ggcgactcgc tgacctttgc cggggagatc
     6901 ctgcatggcc cggagcggct gctggtcaag ccgatccggt tcctgtcggt gatcctctac
     6961 cctggcgcgc cccagtgagc gggctcgatc aggcgctccg gtacattcgt cccgaacagc
     7021 agcaccgcac tcgccaggag cagcccatgt ccagattcca cgccgccgca gccgcctcgc
     7081 tgcttctttc gttcgtcagt cttcagccgg tacaggccgc cgaggtcggc gagcagtggg
     7141 agtacagcgg cacgatggac atgatgggca tgaagatgcc gatgccgccg tcaaaggtct
     7201 gctcccggcc cggggccgaa tcgacgccca ccgctgacaa gcgctgcaag gtcagcgacg
     7261 tgaagacgac cgccagcaag accagctacc gcgtctcgtg cccgccgccc gatgcgatgg
     7321 aaggcagcgg cgaatccgag cgcaagggcg acaccgtggt gtcgtcgttc cgcatgaagt
     7381 ccaaggacgg cgagatgaag atgtcgatga cgggccgcaa gctcggtccg tgcaccccgt
     7441 cggcgaactg accgcctgac ccccgcatgc tgcgaacact gaaagatctt ttcgacgcgg
     7501 tgatgccgcc gagggcgggc gatacgcccg atcacaccga gcatgcgatg cacctggccg
     7561 ccgccgtcct gctcgtcgag gtgatgcgat cggatgccga gatctcgcag gacgaacgca
     7621 gcgcggtcct gtcggcgctg caggcgcggt tcaatctgtc cgacgatgag ggtgcccgcc
     7681 tgctcgaact ggcacatcaa gccgcgcacg gcgcgaccga ctacttccag ttcacctcga
     7741 agatcaacga ctcgttcgac atggaccaga agatcgtcct ggtcgagcag atgtggcagg
     7801 tcgcctacgc cgacggcgtg ctcacggcgc acgagaacca cgtgatgcgc cgcctgagcg
     7861 acctgctgca cgtgcctcac ggcgcatatg tcagcgcgaa gatgcgggcc aggcaggccg
     7921 ctggggccgc ctgacggcgc cgcctccagc cgaggcgatc agcggctcag ggccgcaacc
     7981 ggcgcgatgc gacgcaccag cggggccagc gcaatcagcg tggcgcacag catcaccacc
     8041 tcgatgccgt agacgaagat gtagccggtg gccggcacat tgagcgcatg acccagtcgg
     8101 tcttgctccg ccatgctggc aaccacatcg cgaatgatgc cgcccatcgc gaccgccaga
     8161 ccggcggcag aagcctgcac cgcaccccag gcaccgagcg ccagcccggt ctggttcttg
     8221 ggcgcgagat tcatcgtggc ggtcagagtc ccgtgcccga acagtccggc gccgaagccg
     8281 atcagcatcg tcccgaacgc gaacaggatc ggcgaatcag ccggtgccgc gaagatgacc
     8341 gccaggaagg ccggtatgcc tgccaacgca ccgtagcccg ccatccggaa cggatcggca
     8401 ccccggctca ggacacggga cgcgagaccg aaaccgagca gcccgccgac ggccagcgtc
     8461 gcggtcaact tggtcgtggc ggccacgctg agctgcaaca cctggccgcc gtagggttcg
     8521 agcaggatgt cgttcatgct gaacgccatg gtgcccagtc cgaccgcgca aagacggcgc
     8581 accgcgttcc cgccctggca gaagctgtcc cacgagtcgc ggaatgactg ctccttcacc
     8641 gttccggagt tccgggaagg attgcgcttc tcctgcttcc acatcgccgc gatgttcagg
     8701 acgatcgtca cgacggcggc accctggatc acctggatga gcttggccgg cgtgaaatcc
     8761 gacagcagcg cgccgaagac cagggagctg acgatcatgc ccatcagcag catcacgtac
     8821 atcaggccga cgaccttcgg ctgggactcg accggcgcca ggtcggtggc cagcgccagg
     8881 ccgaccgtct gcgtcatgtg cagcccggcg cccaccagca ggaaggccag cgccgcgccg
     8941 agttggccga accaggccgg ccactggctg gcattgccgc cacccgaaag caccagcagg
     9001 gcgaacggca tcatggccag cccgccgaac tgcagcatcg tgcctttcca caggtagggc
     9061 acgcggcgcc aaccgagtgc ggaggcgtgg gtgtcggagc ggaagccgat cagcgcacgg
     9121 aacggcgcga agaccagcgg catcgcgatc atcaccgcga ccagcgatgc cggcaccccg
     9181 agctcgacga tcatcacccg gttgagcgtc ccgacgagca gcaccatcgc catgccgacc
     9241 gacacctgga acagcgacag gcgcagcagt ttcgacaggg gcagatccgg cgtcgcggca
     9301 tccgcaaacg gcaggaatcg agaacccagt ccagcccaga cgcgcaacag ttgctggttg
     9361 aggcgattca tcgttcaggt cgtggaaaaa caggccggga tgatttatcc cggcctttca
     9421 aggcacaaca gaagtcaagc gaacttactt ctgcggcgtt ggagcctgcg acacgatcgt
     9481 gtccggcgcc aaagctgcgg ccggcttggc agctgctgcc gctgctgcct tctgaccgcc
     9541 gttccagtaa gcgccaaacc agtccgtgtt gatcatcaga ccgccgtgca cgatgagcga
     9601 gcccaccatg acagagccca gaaagatggg cacaccgatg gttgggttaa ctacgcgcca
     9661 aacttttcct tggttcatgt tggatccttc ttaatgaagc cagggcgtgt aggcgtacgc
     9721 gagcacgtgg gccaggatgg caatgaagaa gaacacgcgt gtgccttcca caaggccgcg
     9781 ctgcaattct tcagcttctg ctgtcgtcag gccggacggc cagactcggt tgggatcatc
     9841 tgccataggt atctcctcga gtgagtgatg agcgttcccg tgctgaacca gcacgtcggt
     9901 actgaacgga tgggatggac gaaccaccac cctgcgcaca gccaccccga cattagccag
     9961 atgacttaca actgtcaatt cttgtttact gtcgaaactg tcacgttata ttgacagttt
    10021 tgacctcgcc tcaacctgcc ctgttgatca ggttttcccg aatcgggcct tcgctgcggt
    10081 caggcagccg gccttggcat cgccggcgag cgcgtcgcac ttttcggccg ccaccttgta
    10141 gtcggcatcg gtcttcgact gggaagcatc cgaccgggcc tcgtcgacct tcttgtccag
    10201 cttggcatcg gcgagtgcct tggcctcgac ggccttggct tgcttgacgc agacatcctt
    10261 cgcgttgccg gccatgtcgt cgcacttctc cttggccact tcataggcgg cctcagcctt
    10321 cgccttcatc agcttgttgt gatcggcggg cttgccggtg tagttgtatt cgagctcggc
    10381 cagcgcaacc ttctcgttgc ccttggcttc ctcttcgcag atgtccaggg cgttgccggc
    10441 cagcgatcca cagcccgtct tctgggcctt gtattccgaa ttgatgcgga tctttgccgc
    10501 cttgtagtcg gcctccgcca tcgacgcggc ctgagccacc ggaagcgcca gcagggcggc
    10561 gatgcagagt gtggtcttga ggttgaagtg ggtgctcatg ggagtcctgg tttgcgtcac
    10621 gacaatgtga tgcaggcact ctgcctcgcg cagccccacg cgcacgccgg aacccaccgc
    10681 cgctcgccgt agccgatgtc ctacgcccgc agggcggtgg cagggcgccg ccgcattgcg
    10741 gccggcctca accggaggcc gaccacccgt gcgccgccct gccggcgacc gccgacatcg
    10801 cgctcagggc gctgccccag gccatgtcga tcagtgccag gccgatcggc caggatctca
    10861 gcgtcgccag gttgctcagg tcgtaggtgg cgtaggccac cagcccgaac agtgcggccc
    10921 ggctcgccgt gccgctccat cccgagtcgc cgacgtgcgg catgacggcg aacaccgtca
    10981 ggcccgacgc gtacagcacg tagaacgcgc cagctgccca gaggttgggt cgctcggcca
    11041 tcaggtgccc gattccgttc tggtacatcg gtttggcgat gaagcccagc cacaggacgt
    11101 cgagcgcgat catcaccagc aggatcgccg cgaacgcggg caggtagcgg ctcatggctt
    11161 cgcgctcacg cgccagacca cgttgcccac gtcgtcggcc acgagcaagg cgcccttgcg
    11221 gtcgagcgcc acgccgacgg gccggccgtg cgcctcgccc tgcctgctca ggaacccggt
    11281 caggacctcg accggcgcgg cgcccgaggg cttgtccccg tcgaacggga tgaaaaccac
    11341 ctggtagccg ctgtggggct cgcgattcca cgaaccatgc tggccgacga acatgccgtg
    11401 cgcaaacggc gccggaaggc tggtgccggc cgacgaagcc agccccagcg aggcggtgtg
    11461 cgggcccagc gcgtaatcgg gcgagacggc gcgcgccacc atctcgggct gcggcggctg
    11521 cacgcgcacg tcgacgttct gcccgtagta gctgtagggc caaccgtaga aggcgccgtc
    11581 gcgcaccgag gtcaggtaat cggggacgag gtcgctgccg atctcgtcgc gctcgttcac
    11641 gaccacccag agcacaggcg cggaagatcc gtcgccgcga gcacccgtcc aggcgagacc
    11701 gttcgggttg cgcaggccgg aggcgtagat gcggtgcgca ccgctggcgg cgtcgacctc
    11761 ccagatggcc gcacgctgtg cctcggcgtc cataccgtgc tcgcccacgt tgctgttcga
    11821 gccgacggtc acatagagct tgctgccatc cacactggcg atcaggttct tcgtccagtg
    11881 gtgattgatg gccccccccg gcagatcgac gaggtgggtg gcgccggcag tgatgcgggt
    11941 ggtgtccggg gtgtacggga acttcaccac cgaatccgaa ttggcgacgt acagcgtgtc
    12001 gccgaccagc gccatgccga agggagagcg cagcccctcc agcagcacgg aacgcaggtc
    12061 agccacgcca tcgccgtcgg tgtcgcgcag cagggtgatg cgatcggcgc tgggcacgcc
    12121 ggcccccgcg cgcttcatca cccacccggc cacccagccc ttgatgccct ggccatcgct
    12181 cggcctcggc ggagcattgc tttcggcgac gaggacatcg ccgttgggca gcacgagcag
    12241 ccagcgagga tggtcgaggt cgcgagcgaa ggcggacacc tgcgtgcccg gcgcggcctg
    12301 cggcgtcagg ccttccggcc atcccaccgc cggagcgatg ttcacggtcg gtatcagcgc
    12361 cggccgcggt gccggcaagg tgggatgcgg accgacgccc gccgacaacg gcagcttcgc
    12421 tacatcgccg caggcggcga gcgaaagcac cgcgcagatc accaggatgg ggttcaggct
    12481 catcggcacc tcccgaggtt tcaacatgga acgctgaggc tgacggccga acccatcgga
    12541 catcagagtc gcccggtcag caccagcacc agcacgatga tgacgatcag gcccagacca
    12601 ccactgggcc cgtagcccca gcttcgactg tgcccccaac tcggaaatgc gcctatcaac
    12661 agcaggatca ggatgatcaa cagaatggtt cctacgctca tgtgcttctc ccgggatggt
    12721 tgagggtgtt cgacatcagt tcgccggcgc cgaggccgcc ggaggcagca cgatcaccgt
    12781 ggagccgtcg ccggtggagc ccgtggcgcc cttgttgccg tcggctccct ggtttccggt
    12841 ggcaccggat tcgccctggg ggccggccgg gccgggaact ccgactgcag gtggcggaac
    12901 gttgacgacg gtcggcttgt cgcaggcagc gaggctcgcg gcgacgagca gcgccgaggc
    12961 cagtttcaga tgattcatga cagatcctga cgtggtgaag aaagcggaag acggagcctg
    13021 cgcggacgga cgcgtgacgc ggcgccacgg aggaggctgt gcatctgcac tgcctcgggt
    13081 caggctacgt gcaggcggcg cggcagtcgg tgcggtggcg aacgcaagac ccggactccc
    13141 gccagcgctt gatcaggctc ccagcatgcg gcgcggatcg acccagccat cgaactgctc
    13201 ggcgctcacg gcgccggatg ccagcgccgc gtcgcgcagc gaagtgccgt ggtcgtgagc
    13261 actcctggcg atctgcgcag cccggtcata gccgatgtgc ggggccagcc cggtgaccag
    13321 catcagcgag ccctgcagca tctgctcgac gcgggccgag ttggccgaca ggccggcgac
    13381 gcaatggcgg tcgaagctgt tcatcgcgtc ggtcagcagg cgcaggctgt cgagcgtgtt
    13441 cagcgcgatc aacggcttgt agacgttcaa ctcgaactgc ccctggctgg ctgcgaatcc
    13501 gatcgccgcg tcgtgtccca tcacctgcgc gcagaccatc gtcagcgcct cgacctgggt
    13561 cgggttgacc ttgccaggca tgatcgaact gccgggttcg ttctcgggaa gacgcagctc
    13621 gcccagcccg gcacgcggcc cgctgcccat caggcggatg tcgttgccga tcttggtcaa
    13681 tgcgatcgcc agcatcttca gcgccgcatg aaaggcgacc agcgcctcgt ggccggccat
    13741 cgccgcaaac aagttgtccg cctgaacgag cggcaggtcg agctcgcgcg ccagcagggc
    13801 cgccacccgc gagccgaatt ccgggtgtgt gttcagaccg gtaccgaccg ccgtgcctcc
    13861 gaccgccagc gcatgcaccg ccaccagcgc atggcgaagc gcctgctggc acagcgcgag
    13921 ctgggcttcg tagccgccga actcctggcc cagcgtcacc ggcgtcgcat cctgcaggtg
    13981 ggttcggccg atcttcacga cgaactggaa cgcgatcgcc ttggcggcca gcgcggcgcg
    14041 caattcagcc agtgccggca gcagccgatc gcgtgcccgc aaggccaccg ccaggtgcat
    14101 ggctgtcggg aacacatcgt tcgacgactg cccgaggttc acgtcgtcgt tcgggtgaac
    14161 cgcgcgcgcg gcagtcgctg cggcgtccac cccgccgact tcaccgcccg cgagcgcctg
    14221 cgatgccaga tgagccacaa cctcgttgac gttcatgtgc gtctgcgtgc ccgaaccggt
    14281 ctgccacacc gacagcggga agtgagcatc gaactcgcct gctgcgacac gcagcgccgc
    14341 atcggcgatg gcctgcgcgc gggactcgcc gagcaggccc aggtcgcggt tgacgcaagc
    14401 cgccgcccac ttgacccagg ccaccgcgtg gatcagcgcc agcggcatgc gctgctcgcc
    14461 gatcgcaaag aagcgcaggc tgcgctcggt ctgtgcgccc cacaacgcgt cggcgggcac
    14521 ctcgatcggg ccgaagctgt cggattcgat gcgtgtcgaa ttcatcagga ctccttcagg
    14581 acttgggcgc agcgtggcgg cgcatcaggc tgcggcaggc gtcgccctgg cgctgcgcga
    14641 ggtcggcatc gccggtgtcg atcagcggaa tccaggcggc cagcggagtc agcagggcca
    14701 gcagcaatgc agaggccacc ttgcccgaga gcggcccgcg ttccagcgtg aattccgggt
    14761 cggcaaaggt gccgcgcacg cgcagtggcg agcgcaaggt cagcgggctg aaatccttcg
    14821 ggctgaccac cgcacgcagg tcgagtgatt cggcggccag cgacagcgaa ccgtcgatcc
    14881 acaccgccga atcggtcgta tcgagcacca tcatgcgggg gctgaacacg ccacggacgg
    14941 cgttcaggtc tgccaccgcg cagccgagcg gaagcgcatc gtcgttcttg aacaggacgc
    15001 ccagcgcctg ggccacgtcc aggccggcag cctcgacgac caggtgcgac accgaaccgc
    15061 cgctgagttc gccgcgaacc cggccatcga ggctggccag gatggcggcg gtcgatcggc
    15121 cctgcccttt caacgtcgcc cgcccgccca gacgtcccga cacatagggc ggcgcgcccc
    15181 cggtgcgcgc ctggcggatc cagcgttcga gccgcacatc ggtccagcgc aggtcggcgt
    15241 cccacatcgc ctcgctgccc cggccatcga ggctccagcg ccccttcagg cgaccgtctg
    15301 cggtgcgcgc atcgaggccg ctcaggacca gcacgcccgc ggtcagctgc aggtgggcgc
    15361 gcaacggacg cagcggttcg agcagggtgg tgttcaggtc gacctcggcg atgtcgatca
    15421 gcacgtttgc gtccatcgcg cgcagcgccg cgagatcgaa cggtcggacc ggcagcacct
    15481 tgcccgccac cttgcccgcg acctccgcca ccggcagcgg tgacgaggcc gccgccatcg
    15541 gagccgcagc cgtcgtgccc accaccggac cgaggtcggc cagttgcagc cggctgccgc
    15601 caagacggcc cgacagcagc gggaccgcat cgccgacgcg gtagccgaac gcgccgttca
    15661 ggcggctcga ccccacggtc acgtcgtcga gtcgaacctg ccactcgccg cccttcctga
    15721 cgaccacgcc gcgcgcggcg aaggccgcgg tggtcggcaa ggtcacgccc accgggtcgc
    15781 ccacggcagc cagcgatggc cccttgaggc tgaagcggcc gctgaacccg ttgaagtcca
    15841 gcgcatcgtc gacgacgccg tcgaatgtga cgcgagcgcg gccgacggtc gcatccagca
    15901 ccacggggct cgacaccgtg cgcaactcgc cggtggtggg cgcaagcacg tcggaggcca
    15961 gcaggtcgat cttcagcggc agtgcgcgat agcgacccgc agcactgagc ttgagatcgc
    16021 tggcgaccag tgacagccgg gcctcgacat cgatcgccag tggcgtatcg cggtagctca
    16081 acacaccatc ggacacctgc aggcgcccga aggacgggat cgaaaccggc tgcttcggca
    16141 agcccgccgg ctcggccgac aactgccagg tcgcccgtcc gtcggccagg cgctcgagct
    16201 gcgcgtcgag cacgctggcc tgcagggatt cgatacgcag cgtctggcct cgataggcgc
    16261 gccacaggtc gatgtagcgc aggcgcagtt cgacatcgcg cgccagcagc aggtgcggct
    16321 gcccgctcca ggcgggcgct ccgatgacaa gcagtggggt gtgcaggcgc acgccgccga
    16381 cgaaacggac accgaagcgg cgcgacgccg cggcctgcgt gtcggcagct tgcgaaagag
    16441 tgacacgccg tgacgtggcg ctcgacagcg acttctcgag cggcgcagcc aggaagggcc
    16501 actcgagccc ttcgccggct gccagcaggc acatcaggcc acccagcacc agcgcagccc
    16561 agatcagccc gagccgacgg cgcggcggcc tgccggcacc cgctgcgagg gcgtcagcct
    16621 cgccgggctc agaggtgcgc gagatgaccg tgctctccga ggccgatcgg cttggcagcc
    16681 accaccgaca ccgcggtcgc gaacagcctc accatcttgg tgtcgaccgc gtcccagtac
    16741 tccgccgagt gcggcgtgaa cttcagcagc gtcagctcct cggagtcggg gccgtgcggg
    16801 aaccacgggc gcgccagcgg cgaccacaac cgcttcacgt gctcgcgatc gtcgacgatg
    16861 tcgccgtgtc cggacatcga cacataggtg ccgcgttccg catcgacgaa acagaggttc
    16921 agcgctttca actgctcgat cttggctgac cggcggtgcg tgaagaacca cagggcgccg
    16981 agcgcgtcca tctccagcgg ggccatcggc cggctgacca gcgcgccatc ggcgtcgtgg
    17041 ctggtcagca tggccaccgt gtccatcggc tggatcagct gggccaggtg gatcagttcg
    17101 gcactggatt ggctttgaat cttcatgatc aactcctctt gaggttaaga aggtcgttcg
    17161 cgagttcgtc ctcgtgggac gcgaacggcc gctggggcgc gccgcctgcg tgatcagcgc
    17221 agcgcgtcga tgaagacact ggtgtcagcg tcgtcgcggg tgagcgtggt ggttgagctc
    17281 gagagtgcga ctctctcgcg ggatcgcgga gcgatcgggg ccggaactgg tcgggtcgtc
    17341 catggcgaca tccctcctgt cagggtgcga gcggccagct tcacgcaggc gtgtctccgc
    17401 gtatgtcggt gaagccaagg ccctgttgta atccttggcc tacgccatgc gggcaggagt
    17461 gtttcctggt gctgacgcgc gcggcggatg cttgccagca tgagcgcggg gcacggcgat
    17521 gcagcgcggc gggcgccgcg cgccggtcag tacagattca cttgacgagc agaaccggga
    17581 tgtcgcagtc cgtgatcacc cgctgtgcaa cgctgcccat cagcgcccga ccgatcaggc
    17641 ccagcccgtg cgtgcccatc acgatcaggt gggcttcttc gcgcttcgcc gccttgacga
    17701 tttcttcgcc aggtgtgccc accacccagg cggtgcggta gcgaaccggg tggcgatcga
    17761 ggaagcgccg gaccggcacg agcaccttgt cggcctcgtc actggcgtac tcggtgatcg
    17821 ctgcggaccc cagcacccgc ctcacgtgcg ggggcagctt cgcctgcacg ttgaggacga
    17881 acagttctcc ctgctcacca agcagcccct catgtgtgac caggaatgcc aatgccttct
    17941 tggtgcactt gctgccgtca gcggcgaaaa cgatcttcat ggagggctcc ttgatgtggc
    18001 gggcgagtcg agtcgcccgt cggtcactgc tttgccggcg tcgcgggcag gcgatccagc
    18061 atcatctgca tcatcgattg catcatgtcc agacgctttt cgatggcctc gtgatgcgcg
    18121 gccatgttgt catgcatcga gcccttgcct tgcttcgcgc cgtggcactg catgccgccg
    18181 gcaccggcgc cacccatcat cgccatgccc tcctgcatgg ccttcatgtg ctcggcccgc
    18241 agtgcgctgc gctcctcggg cgtcttggcg ttcatcattt tctcgtgcat ctcgcgcatg
    18301 tgcttcatgt gctcgtccat gcgcgaggcc tctgcggccg caccctgggc atcgcctggc
    18361 gccgacgcag acgcagcagg ctgatgcgag gaatggtcgg cagccgcccg ggcggcgggg
    18421 ccggcgagac agagtgccgt ggcgactgcg gccagcgcaa gatttcggat cgaggtcatg
    18481 ggaactccag tgtggtgagg ccgagcgccg cggtgccggc ggtccacggc gggcagcggg
    18541 acagccggtg gaaactcgct gcgcggttca acgcatgacc accgccctgc ccttccatgc
    18601 taggccgaag gacctcgagc cgattgatgc gcatcaacgg gccggtcgat cacgtgaaac
    18661 gggtccggga tcacacggct acaggcgtgt agcccgcatc ccgtatcgcg tccccgaggc
    18721 tggcagcgcc agcctccgac gactcgatct ccacccgatg ctgcgccaga tcgatgcgca
    18781 cttcagccgt gctgtcgacc gccttcaacg cctgggtgat ggcgttgacg cagtgaccgc
    18841 aggtcatgtc cttgacttcg taggcaatca tgtcgatctc cagaaaacga acagggtaag
    18901 gcccgcagga tgtactgtcg aggacccctc ctcgaatgat cgcctgcaca actgcccatg
    18961 aacaccaccg cctcggcaaa ggaacagggc agcgccctcc agctgcagat caccggcatg
    19021 acctgcgctt cgtgcgcgct gcgcgtggag aaggcgttgg cgaaggtgcc gggggtcctg
    19081 caggccagtg tcaatctggc gaccgagcag gcctgcgtcc gtgccgacgc ctccgtcggc
    19141 acgcaggtcg tcgcggatgc ggtgcaccgg gcgggctatg gcgtg
//
LOCUS       DAOORV010000067        18937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3548_length_18937_cov_7.079917, whole genome shotgun sequence.
ACCESSION   DAOORV010000067 DAOORV010000000
VERSION     DAOORV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18937
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3548_length_18937_cov_7.079917"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1540)
                     /locus_tag="PKV56_10365"
     CDS             complement(56..1540)
                     /locus_tag="PKV56_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HOX68259.1"
                     /translation="MSGALVWFKRDLRVADHAPLAEAMHCDAALALFVIEPAWLASPE
                     CHARHVAWLLKCLVPLREALAKRGLALLVRTGDAIEVLATLRSEFGFTHLFSHEETGP
                     GWSYEREKAVAHWCRAQGVAWTEWPQTGVIRRLKDRRGWAGRWQQRMDAPCVPVPAGF
                     NAAPGFTPSSVHSGLPTLRDLGVSPSAETSQAGEAAGQAALDTFISGRALGYRRRLSS
                     PLAAQTGCSRLSEHLAFGTLSMRTVHQSTEAAIRSTPDGELAHGLRGFASRLRWHCHF
                     MQKLEDEPAIEWRNFARSADGLRPGDGTELRPIDRERLQAWCEGRTGFPMVDACMRQL
                     IATGWLNFRMRAMLVSFAAYHLGLHWREPGLHLARQFLDFEPGIHWSQMQMQSGCTGI
                     NTLRIYSPAKQARDQDPTGDHLRRWLPEFGTPAYPAPIVDEREAVAEIKARLHQLRQT
                     APARAEADALQQRHGSRRSGLPSTARPSRRPRAAAVPDSQGRLF"
     gene            complement(1567..1698)
                     /locus_tag="PKV56_10370"
     CDS             complement(1567..1698)
                     /locus_tag="PKV56_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68260.1"
                     /translation="MILNDIALVTDKPGGVGRALVARRNAPVVAVAGGFAAVRPPAQ"
     gene            complement(1730..2668)
                     /locus_tag="PKV56_10375"
     CDS             complement(1730..2668)
                     /locus_tag="PKV56_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HOX68261.1"
                     /translation="MTSPARNDTLTVLYDGACPLCQREIAHVRDLAQRKGNSALCFVD
                     ISRDDDVTTPAERAALLARFHVQRADGTRLNGAAAFVAMWSRLPGWRWLARLARLPGM
                     LSVLELAYSGFLHVRPTLQVLARRWEPSARTGAGLSLHMERELRSDHAGETGAVCIYR
                     GIAAVAAWRGDAELLAFARRHGDTEAEHLRLVEEWLPRQRRSRLLGPWRLAGWMTGAL
                     PALAGRRAVHATIAAVETFVDRHYQQQIDHLQRRDGQHEIDGLRPLLQRCQADERHHR
                     DEAAALAGARLPWSLRTWCTLVAAGSAAAVVLARRI"
     gene            complement(2730..4271)
                     /locus_tag="PKV56_10380"
     CDS             complement(2730..4271)
                     /locus_tag="PKV56_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HOX68262.1"
                     /translation="MADLRHLVIVLGDQLDLDAAAFDGFDGAHDAVWMAEVTEESTHV
                     WSSKSRTVMFLAAMRHFAQALRAAGRPLHCVRLDEPDNHGGLQAQLHADIERLRPTGL
                     VMTAPGDWRVLQAINGAAQAHGLPLDIREDRHFYCTVREFAAHAKGRKSLRMEYFYRE
                     LRKRHGVLVDGDGPEGGRWNFDEDNREAFGAQGPGAVPSRARFKPDAITREVIALVEE
                     RFAAHPGRLEDFAWPVTRDQALEALQRFVTERLPLFGRYQDAMWPGEPWLHHAHLAAA
                     LNLKLLNPREVVDAAVAAYRAGTAPLASVEGFVRQILGWREYVRGIYWTRMPGYAALN
                     ALEAHEDLPAWYWTGATHMACLRDAIGQTLQHGYAHHIQRLMVTGLFALMYGVHPQQV
                     HAWYLAMYVDAVEWVELPNTLGMSQYADGGLMASKPYVATGKYIQRMSPHCKGCRYDP
                     AQRTGETACPFTTLYWDFLMKHETALAGNPRMALQVKNLGRLDDTQKKAILARAAAVR
                     SGEVA"
     gene            complement(4264..4437)
                     /locus_tag="PKV56_10385"
     CDS             complement(4264..4437)
                     /locus_tag="PKV56_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HOX68263.1"
                     /translation="MTMPATPPAFRGNKAALPSKPCLTCCRPMSWRRRWARTWNEVRY
                     CSDACRRARADHG"
     gene            4631..5680
                     /locus_tag="PKV56_10390"
     CDS             4631..5680
                     /locus_tag="PKV56_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX68264.1"
                     /translation="MASLHRSGAVARMLKMPVATLRVWERRYALTNTSLSPSGQRRYS
                     ADDVRRLAFLKQLTDLGHAIGSLASLDLAQLQRVAATHAQALAAVQPQRQAADASSSR
                     SRPWRLVVVGNGLGARLHRQSLLRQLGRPVEILGPFEDVPQAAAALATRSDGRPADAW
                     LLHQPQLHADWLAEFEAVVLGSTSLPEQERAPVAVAVLFSYAADAVCDALAAGGVALL
                     REPQPDAALAQWLRLWVEASAVPASGPFSSLAPASDSGAAAAPRRWDDGALADFASRS
                     STVVCECPRHVAELLMQLSRFEVYSAQCEQRSAADAELHAFLGRVAADARASFEVALE
                     QVALREGLLLPRSPV"
     gene            complement(5802..6746)
                     /locus_tag="PKV56_10395"
     CDS             complement(5802..6746)
                     /locus_tag="PKV56_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOX68265.1"
                     /translation="MPVYSMTGYASATAGTPEAPASTANSDHPVARPVTPARTSVTVE
                     LRSVNARFLDLSLRLPDELRALEPALRDLLTASLRRGKVELRLNAQTSSDTAWPQPQP
                     EQLNRLSHVQSTIRGWMPTARELSINEVLNWCKAGSVTERLDEAALDAARNAIDGLRE
                     ARAREGQRLVDVLMDRVDRLRELAARAEPLVPATVARQQQKFLERWREALEQAGATQT
                     VSREALQERALNEAAAFAIRIDVAEELARLRSHLDEMARMLKAGGEVGKRLDFLIQEL
                     LREANTLGSKASSLELTNISLDMKVLIEQLREQVQNIE"
     gene            6777..7745
                     /locus_tag="PKV56_10400"
     CDS             6777..7745
                     /locus_tag="PKV56_10400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOX68266.1"
                     /translation="MSKLKPAPLPSGTVVGGYQIVKKLAAGGFGVVYLAEDAQRHMVA
                     VKEYLPSSLAERSQGELTPRVKPEKQPLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RENETVYMVMNYLQGETLQDFIVTARDLKRDKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITNDNRAVMLDFGAAREVLSKEGNFIRPMYTPGFAAPEMYRRDGTLG
                     PWTDIYAIGACIYACMQGYPPNDSPQRIEKDRLALSLSRLRNVYSDNLLEVTEWCMSL
                     DPLSRPQSVFALQKELARETERRYTKLTFGERLKLQLENLTTGAKA"
     gene            7783..8700
                     /locus_tag="PKV56_10405"
     CDS             7783..8700
                     /locus_tag="PKV56_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HOX68267.1"
                     /translation="MRFSIYQVSRKGGREKNEDRMGYSYTRDSGLFALADGMGGHPEG
                     EVASQLALQTMSALFQQASKPTIPDPLAFLNQAILAGHQQLLRYSAQKALSDTPRTTV
                     VACLLQGDSVYWAHCGDSRLYMVRAGKLIARTRDHSYSELQDALHSVVPMRERLNRNV
                     LFTCLGSPGKPVIDTAGPFTLQGGDRLMLCSDGLWGTVTDESITEELGRRSIAEAVPE
                     LVEQALRNGGPKCDNVSVMAVEWECSEAEQGGSGVSTEELGDDVFASTIQASVLGHDA
                     ADDLDDAEIERSIKEINDAIRRSSQKKKN"
     gene            8712..9437
                     /gene="rph"
                     /locus_tag="PKV56_10410"
     CDS             8712..9437
                     /gene="rph"
                     /locus_tag="PKV56_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOX68268.1"
                     /translation="MGFERTQGRSVDSLRPVRISRHYTKHAEGSVLVEFGDTKVLCTA
                     SVEEKVPPHQRGSGRGWVTAEYGMLPRATHTRSDREAAKGKQSGRTQEIQRLIGRSLR
                     CVFDLSLLGERTISLDCDVIQADGGTRTAAITGAFVAAHDAVSGLIAQSKLGASPIRD
                     FVAAVSVGIVDGVPLLDLEYIEDSACDTDMNVVMTGSGGFVEVQGTAEGASFSRDEMN
                     SLLQLADRGIRDLVAAQRRALGL"
     gene            9450..10064
                     /gene="rdgB"
                     /locus_tag="PKV56_10415"
     CDS             9450..10064
                     /gene="rdgB"
                     /locus_tag="PKV56_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOX68269.1"
                     /translation="MRLVLASNNAGKLAELQALFQPLGLDLVRQGELGIAEAEEPHLT
                     FIENALAKARHAAQQARCAAIADDSGLCVDVLGGAPGVRSARYAEDFGQPKGDASNNR
                     VLLEQLAGVTSEVERVARFVCALVAVRSADDPQPLVAMGRWSGVILPAPRGEAGFGYD
                     PLMFMVELQRSVAELSPSEKNGRSHRGIASREMLRQMREVWGLG"
     gene            10186..11337
                     /gene="hemW"
                     /locus_tag="PKV56_10420"
     CDS             10186..11337
                     /gene="hemW"
                     /locus_tag="PKV56_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOX68270.1"
                     /translation="MSLYVHLPWCLKKCPYCDFNSHEHRSSAANELPEARYLDALRCD
                     LEAALPFIWGRRIHTVFIGGGTPSLFTPDSIGQLIADIRARLPLEPGCEITLEANPGT
                     FERDRFRAYRAAGVTRLSIGVQSFDDAKLAALGRVHDSAQARAAVAEASEAFETFNLD
                     LMYALPGQTLPELEADIATALSFKPPHLSIYHLTIEPNTLFAKHPPAGRLALPDDDIA
                     FDMLDVITQRSEDAGLSRYEVSAFARAAHRCAHNLNYWQFGDYLGIGAGAHSKISYPH
                     RVVRQVRFRDPGSYMSRALAGQAVSQDEEVPRKQLPFEFMLNGLRLKEGIELARYTER
                     TGLPLSSIEPQLAEATQRGLLVREPNRIQPTPHGYDFLNDLQELFLPAS"
     gene            complement(11359..12438)
                     /locus_tag="PKV56_10425"
     CDS             complement(11359..12438)
                     /locus_tag="PKV56_10425"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOX68271.1"
                     /translation="MAKTRVVVVDDSALVRGLLTEIINKQPDMECVGAAADPYIAREM
                     IRELNPDVITLDVEMPRMDGIDFLGKLMRLRPMPVVMVSTLTERGADVTLRALELGAI
                     DFVAKPKIGVADGLKLLAHDITDKVRIAAKAHLRRPAAAPAVGAAPRPSSPPSAIGRL
                     STEKIIFIGASTGGTEATKEVLVNLPADCPAVVITQHMPPGFTKSYAARLDGLCKVRV
                     AEARDGDRILPGHAYIAPGGFHLSVERSGANYIARVQDGEPVNRHKPSVEVLFKSAAR
                     VVGPNALGLMLTGMGADGATAMREMRDAGSYNLVQDEASCVVFGMPREAIAAGAANEV
                     LPLTQIAPKLIERLRSTAGMSMNRV"
     gene            complement(12477..13199)
                     /gene="cheD"
                     /locus_tag="PKV56_10430"
     CDS             complement(12477..13199)
                     /gene="cheD"
                     /locus_tag="PKV56_10430"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HOX68272.1"
                     /translation="MNMPIGQNTFAASSPSRLRPSPLAGGSSTPGIGQPAPGSRLARL
                     RAQTPKPGQASFFFYEAHFKSDAVKVLPGEYYVDNEDLLIMTTLGSCIAACLWDRNAR
                     VGGMNHFMLPDGVGDSGRYGSYAMELLINEMLKLGASRSSMEAKVFGGGAVISGMSSL
                     NVGERNTTFVLDYLKTERITVVSKDVLDIYPRKVCFLPASGKAMVKRLAPTSTDALMA
                     QDKVAVERAVKSTSSGGSVDLF"
     gene            complement(13211..14083)
                     /locus_tag="PKV56_10435"
     CDS             complement(13211..14083)
                     /locus_tag="PKV56_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOX68273.1"
                     /translation="MSAVMTATVQTRSDSFVDPTERDLPFDRNDFDRVRRLIYQRAGI
                     SLGDGKQAMVYSRVSRRLRETGHPSFVSYLQWLEQASGPSADTEWQEFINCLTTNLTS
                     FFREDHHFTALVDDLRARASGGIRIWCSAASTGEEPYSIAMTVTEALGANNGTRIIAS
                     DIDTNVLAKAGSGVYPLDSRGLSPERLRQHFLRGKGAQSGLIRVKPELARMIEFRVQN
                     LMDARWSLGDPFDIVFCRNVMIYFDAATQRKVLEKIHATMKPHGLLYVGHSENFTEAR
                     HLFRLRGKTIYERV"
     gene            14318..15034
                     /locus_tag="PKV56_10440"
     CDS             14318..15034
                     /locus_tag="PKV56_10440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX68274.1"
                     /translation="MSTVELSPADLQLQVALQLLKQAGREVSVDAPAGSTPWLQAVVD
                     ALCELSSRDALTGLANRRQFEVTIAREIDRVARAGEPALVLMADIDHFKAVNDTYGHP
                     AGDQVLKAVAAALQDCVRPMDTVARYGGEEFAIILPNCPPAFGHLVAERIRSKVGRRP
                     VNVAPGVDVPVTISIGGAFAPQWVRSSAPLWIERADRQLYRAKAEGRNRVCLEPAAVC
                     QVSAEEKGLLFGTSQFQDLE"
     gene            15045..15860
                     /locus_tag="PKV56_10445"
     CDS             15045..15860
                     /locus_tag="PKV56_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOX68275.1"
                     /translation="MTAPNESTARPSPPAGGRCARTMAVTSGKGGVGKTFISANLAAA
                     LSRRGERVLVLDADLGLANLDVVLNLHPKITLHDVFTGKAELEEAILPAPGGFSVLLA
                     GSGMVEYSRLTSEVRDQLLAIIDKVKPRFDYILLDTGAGISDVVLFAVSLADQVLVVA
                     TPEPTSMTDAYATIKVLATQQSRRAIKLVVNQVGRLGEGRTIRGQLQQVVDRYVSPLL
                     GGPSEALKLELVGEVPTDTAVREAILRRQLLMDMLPGCAAAKAIGLLADKLLA"
     gene            15857..16264
                     /locus_tag="PKV56_10450"
     CDS             15857..16264
                     /locus_tag="PKV56_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HOX68276.1"
                     /translation="MSADSGDAAASTPFELLGGEPAVRQLVDRFYDLMDLEPAYATVR
                     ALHPEVLDGSRDKLYWFLCGWLGGPQLYVEKFGHPRLRARHMPYSIGIAERDQWMDCM
                     KQAMRESRIDETMAARLTESFFGTADWMRNRGV"
     gene            complement(16275..16985)
                     /locus_tag="PKV56_10455"
     CDS             complement(16275..16985)
                     /locus_tag="PKV56_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOX68277.1"
                     /translation="MRPPRKLRSLQDLTALKKAMAESARRVAEARAEAARVQRERDLF
                     ALTVGPVQPLRRGVVIPLERPRAEPLPRQRARDEAAVLREAISDEFDVESLLETDDAL
                     SWRGEGIGPEVVRKLRRGVWSIQAQLDLHGLRRDEARESLAAFLREAVRHGLRCVRVI
                     HGKGNGSPGREPVLKSKVKTWLVQKSEVIAFAQARAADGGHGALLVLLRPTGPTLQPM
                     PPAANDPGPHRSSRARKG"
     gene            complement(16982..17656)
                     /gene="radC"
                     /locus_tag="PKV56_10460"
     CDS             complement(16982..17656)
                     /gene="radC"
                     /locus_tag="PKV56_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX68278.1"
                     /translation="MVLKDIPAAARPREKLLTLGPAALADAELIALLLRTGLRGTSVL
                     QLAQQLLDAFGGVTGLLQASPLDLKRVKGLGPAKRAEISAVIELARRSLSQELAQRTV
                     LSSPQQVKDYLQLQLSHIGHEVFAVLFLDVQNRLIRLEEMFRGTLTQTSVYPREVVKR
                     ALDLQASAVILAHNHPSGVAEPSRADEFLTQTLQSALRLVDVRVLDHLIVGRAAVVSF
                     AERGLL"
     gene            17765..18208
                     /locus_tag="PKV56_10465"
     CDS             17765..18208
                     /locus_tag="PKV56_10465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX68279.1"
                     /translation="MNSVIPGSFLTLHYRLAGPDGADIINTFADKAATLSLGTGELAP
                     AMEARLIGMAEGTRATFELAAGEAFGERNPELMQRVKRALLDELGDPDEQYSIGDVVQ
                     FPTPDGQGAYAGVVREVGGDWVLFDFNHPLASQPVTFEVHVIGVL"
     gene            18208..>18937
                     /gene="ispH"
                     /locus_tag="PKV56_10470"
     CDS             18208..>18937
                     /gene="ispH"
                     /locus_tag="PKV56_10470"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOX68280.1"
                     /translation="MTKPVHVEEVLLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRH
                     EIVHNTYVVNDLRAKGAIFIEELAEVPAGATLVFSAHGVSQAVRQEAERRGFSIFDAT
                     CPLVTKVHVEVAKLHKEGYEFLMIGHKGHPEVEGTMGQLYDGIYLVEDAADVQTAPVK
                     NPDRLAVVTQTTLSVDDAAQVLAAVKQRFPNVREPKQQDICYATQNRQDAVKVLTPQV
                     DVGLPTTITTSTCGVSTSNRLRELA"
BASE COUNT         3013 a         6218 c         6464 g         3242 t
ORIGIN      
        1 ctcctggtga gggcggcagg ggggagggga ccggcaggcg gtgagacgcg tcgcgtcaga
       61 acagccgccc ttggctgtcc ggcacggccg ctgcgcgggg tcggcggctg ggtcgggcgg
      121 tggacggcag gccgcttcgc cgcgagccgt ggcgctgctg gagggcgtcg gcctcggcac
      181 gggctggagc agtctgccgc agttggtgca ggcgtgcctt gatctctgcc acggcttcgc
      241 gctcgtccac gatcggcgct gggtaggcag gcgtgccgaa ctccggcagc cagcggcgca
      301 ggtggtcgcc ggtcgggtcc tggtcacgcg cctgtttggc gggcgagtag atgcgcaggg
      361 tgttgatgcc ggtgcagccg ctttgcatct gcatctggct ccagtggatg ccgggctcga
      421 agtccaggaa ctgccgcgcc agatgcagcc cgggctcgcg ccagtgcagg ccgaggtggt
      481 aggcggcaaa gctcaccagc atcgcgcgca tgcggaagtt cagccagccg gtggcgatca
      541 gctggcgcat gcaggcgtcc accatcggga agccggtgcg gccctcgcac caggcctgca
      601 ggcgctcgcg gtcgatcggc cgcagttcgg tgccgtcgcc aggtcgcagg ccgtcggcgc
      661 tgcgcgcgaa gttgcgccac tcgatggctg gttcgtcctc cagtttctgc atgaagtggc
      721 agtgccagcg cagccggctg gcaaagccgc gcaagccgtg cgccagttcg ccgtccggtg
      781 tgctgcggat ggcggcctcg gtggactggt gcacggtgcg catcgacagc gtgccgaacg
      841 cgaggtgttc ggacaggcga ctgcagcctg tctgtgcagc cagcgggctc gacaggcggc
      901 gccggtagcc gagcgcgcgg ccagagatga acgtgtcgag cgccgcctga cccgctgcct
      961 cgccggcttg cgacgtttcc gctgaaggtg acacgcccag gtcgcgcagc gtgggcaatc
     1021 cgctgtgtac cgatgaaggt gtgaagccgg gcgcggcgtt gaatcccgca ggcacgggca
     1081 cgcacggcgc gtccatgcgc tgctgccagc ggccggccca accgcgccgg tccttcagtc
     1141 ggcggataac tccggtctgc ggccattcgg tccaggccac gccctgtgcg cgacaccagt
     1201 gggccacggc cttttcgcgt tcgtagctcc agccggggcc ggtctcttcg tggctgaaca
     1261 agtgcgtgaa gccgaactcg ctgcgcagcg tcgccagcac ctcgatagcg tcgcccgtgc
     1321 gcaccaggag cgcaaggccg cgcttggcga gggcttcgcg cagcggaacc aggcatttca
     1381 gcagccaggc cacgtgacgc gcgtggcatt cggggctcgc cagccaggca ggttcgatca
     1441 cgaacagtgc cagcgctgcg tcgcagtgca tggcttcggc cagcggcgcg tggtcggcca
     1501 cgcgcaggtc gcgcttgaac cagacgaggg cgccagacat gggggttccg accttccgac
     1561 gcggcactat tgcgccggcg gccgcacggc cgcgaatccg ccggcaaccg cgaccaccgg
     1621 ggcgttcctt cgcgccacca gcgcgcggcc cacgccaccg ggcttgtcgg tgacgagggc
     1681 gatgtcgttg aggatcacgc tgcgctctca cggatggtga aaggtgcggt cagatccggc
     1741 gcgccagcac cacggccgca gccgagcctg cggccaccag cgtgcaccag gtgcgcaacg
     1801 accagggcag ccgcgcgccc gccagcgcag ccgcttcgtc gcggtgatgt cgttcgtcgg
     1861 cctggcagcg ttgcagcaaa ggccgcagac cgtcgatctc gtgctggccg tcacggcgct
     1921 gcaggtgatc gatctgctgc tggtagtggc gatcgacgaa agtctcgacc gccgcgatcg
     1981 tggcgtggac cgcacgcctg ccggccagcg caggcaatgc ccccgtcatc cagccagcca
     2041 gccgccaggg accgagcagg cgactgcgcc gctgccgtgg cagccattcc tcgacgagcc
     2101 tcaggtgttc tgcttcggtg tcgccgtgcc ggcgcgcgaa tgcaagcagc tccgcgtcgc
     2161 cgcgccaggc ggcgaccgca gcgattccgc ggtagatgca cacggcgccg gtctcaccgg
     2221 cgtggtccga gcgcagttcg cgctccatgt gcaaggacag gcccgcgccg gtcctcgctg
     2281 aaggctccca gcgacgggcc agcacctgca gcgtcggcct cacgtgcagg aagccgctgt
     2341 aggccagttc aagaacggac agcatgccag gcaggcgggc caggcgcgca agccagcgcc
     2401 agccgggcag ccgactccac atggcgacga aggcggctgc gccgttcagg cgtgtgccgt
     2461 cggcgcgctg gacatggaag cgcgcaagca gcgcggcacg ctcggcaggg gtggtgacgt
     2521 cgtcatctcg gctgatgtcg acgaagcaca gcgcgctgtt ccctttgcgt tgcgccagat
     2581 cccggacgtg ggcgatctcg cgctggcaaa gcgggcacgc gccgtcgtag agcaccgtga
     2641 gcgtgtcgtt ccgagcgggt gatgtcactt caattccttg gtcagtcagg atccgacgac
     2701 agctcggcag tagggcagca ggagcatgtt cacgccacct cgccgctgcg taccgctgcg
     2761 gcgcgagcga ggatggcctt cttctgcgtg tcgtccaggc gacccaggtt cttcacctgc
     2821 agcgccatgc gcgggttgcc ggccagcgcg gtttcgtgct tcatcaggaa gtcccagtac
     2881 agcgtggtga acgggcaggc cgtttcaccg gtgcgctgtg cagggtcgta gcggcagccc
     2941 ttgcagtgcg ggctcatgcg ctggatgtac ttgcctgtgg ccacgtaggg cttgctggcc
     3001 atcaggccac catcggcgta ctggctcatg cccagggtgt tcggcagttc cacccactcg
     3061 acggcatcga catacatcgc caggtaccag gcatgcacct gctgcggatg caccccgtac
     3121 atcagggcga acaggccggt gaccatcagt cgctggatgt gatgggcgta gccgtgctgc
     3181 agcgtttgcc cgatcgcgtc acgcaggcag gccatgtggg tggcgccggt ccagtaccag
     3241 gcgggcagat cttcgtgcgc ctccagcgca ttgagagcgg cgtagccggg catgcgcgtc
     3301 cagtagatgc ctcgcacgta ctcgcgccaa ccgaggatct ggcgcacgaa gccttccacg
     3361 ctggccagtg gcgcggtacc cgcacggtag gcggccaccg ccgcgtcgac cacttcgcgc
     3421 ggattcagca gcttcagatt cagcgccgcc gcgagatgcg cgtggtgcag ccatggctcg
     3481 ccgggccaca tcgcgtcctg gtaacggccg aaaagtggca gccgctcggt gacgaagcgt
     3541 tggagggcct ccagcgcctg atcgcgcgtc accggccagg cgaagtcctc gaggcggccg
     3601 ggatgtgcgg caaagcgttc ctcgaccagc gcgatgacct cgcgggtgat cgcatcgggc
     3661 ttgaatcgcg cgcgcgacgg cacggcgccc ggtccctggg cgccgaatgc ctcgcggttg
     3721 tcttcgtcga agttccaccg gccgccctcc ggaccatcgc cgtccaccaa cacgccgtgg
     3781 cgcttgcgca gctcgcggta gaagtactcc atgcgcaaag atttgcggcc cttcgcgtgc
     3841 gcggcgaatt cgcgcacggt gcaatagaag tggcggtctt cgcggatgtc cagcggcaga
     3901 ccgtgcgcct gggccgcgcc gttgatcgcc tgcagcaccc gccagtcgcc gggcgctgtc
     3961 atcaccagcc cggtggggcg caggcgctcg atgtcggcat gcaattgcgc ctgcaggccg
     4021 ccgtggttgt ccggttcatc gagtcgcaca cagtgcaagg gccggccggc tgcgcgcagg
     4081 gcttgcgcga agtggcgcat ggctgccagg aacatcaccg tgcgcgactt gctggaccag
     4141 acgtgcgtcg attcctccgt cacctcggcc atccagaccg cgtcgtgtgc gccgtcgaat
     4201 ccatcgaacg ccgcagcgtc gaggtcgagc tggtcgccca gcacgatcac gaggtggcgc
     4261 aggtcagcca tgatcagccc gcgcgcgcct gcaggcgtcg gagcagtacc tgacctcgtt
     4321 ccatgtgcgc gcccatcggc ggcgccagct catgggccgg cagcacgtca ggcagggctt
     4381 gctgggcagg gcggccttgt tgccgcggaa ggctggaggg gtggctggca tggtcatcgg
     4441 gacagacatt tgccggcagg gcggctggaa tttccatacg gatgctatac gttcatggtc
     4501 aaagacgatt cgaaagtgga tattagcctc taaatatcga taaaatgcga gtcaatatgg
     4561 agtcatctgt tcgggttatc gcatgagcga acctgctcgc ccatccaaac cggctgttgg
     4621 agaagaaccg ttggcctccc tgcaccgcag cggggccgtg gcacgcatgc tgaaaatgcc
     4681 ggtggcgacg ctgcgggtgt gggaacgcag gtatgcgctg acgaacacgt ccttgtcgcc
     4741 cagtggccag cgcaggtatt cggccgacga tgtgcgtcgg ctggccttcc tcaagcagct
     4801 gaccgacctg ggccacgcca tcggcagcct ggcttcgctg gaccttgcgc aactgcagcg
     4861 cgtggcggcc acgcatgcgc aggcgctggc ggccgtgcag ccgcagcgac aggcggccga
     4921 cgcctcctcg tcaaggtccc ggccgtggcg gctggtggtg gtcggcaacg gcctgggcgc
     4981 gcgcctgcat cgtcagtctt tgctgcggca gcttggtcgg ccggtcgaga tcctggggcc
     5041 gttcgaagat gttcctcagg ctgctgcggc gctggcgacc cgaagcgatg gccggccggc
     5101 agacgcctgg ctgctgcacc agccccaact gcacgcagac tggctggctg aattcgaggc
     5161 cgtggttctc gggtcgacga gccttccgga gcaggagcgc gcacccgttg cggtcgcggt
     5221 gctgttcagc tacgccgctg atgcggtatg cgatgccttg gcggccggcg gcgtcgcctt
     5281 gctgcgcgag ccgcagcccg acgcggcgct cgcgcaatgg ttgcgcctgt gggtcgaggc
     5341 atcggcggtg ccggcgtcgg ggccgttcag cagcctcgcg cccgccagcg acagcggtgc
     5401 cgccgccgct cctcgacgct gggacgacgg cgctctggcc gacttcgcat cccggtcgag
     5461 caccgtggtg tgtgaatgcc cgcgccacgt ggccgagctg ctgatgcagc tgtcgcgctt
     5521 cgaggtctac agcgcacagt gcgagcagcg cagcgcggcc gatgccgaac tgcatgcctt
     5581 cctgggccgc gtggcagccg acgcccgggc cagcttcgaa gtggccctgg aacaggtggc
     5641 gctgcgcgag gggttgctgt tgccgagatc gccggtgtga ggggcgggtt ccgcccatcg
     5701 ctttcagttc atcgcgaaat tgcaggggct gggccgtggc cgagcgctcg ccggggggcg
     5761 acgaggtacc caagtagcgt gtccggtggc tacccggagc atcactcgat gttctgcacc
     5821 tgttctcgca gttgttcgat cagcaccttc atgtcgagcg aaatgttggt gagttcgagc
     5881 gacgaagcct tggagcccag cgtgttggct tcccgcagaa gctcctggat caggaagtcc
     5941 agccgcttgc ccacctcgcc gccggccttg agcatgcgcg ccatctcgtc gaggtgcgat
     6001 cgcagtcggg ccagttcctc ggccacgtcg atgcgtatcg caaatgcggc agcttcattc
     6061 agggcgcgct cctggagcgc ctcgcgtgag acggtttgcg tggctccggc ttgttcgagc
     6121 gcttcacgcc agcgttcgag aaacttctgc tgctgtcgcg ccaccgttgc cggcaccagg
     6181 ggttcggccc gcgcggccag ttcgcgcagt cggtcgacgc ggtccatcaa gacatcgacc
     6241 agccgctggc cctcgcgtgc ccgtgcttcg cgcagcccat cgatcgcgtt tcgggccgcg
     6301 tcgagtgcgg cttcgtccag ccgctcggtc accgaaccgg ccttgcacca gttcaacacc
     6361 tcgttgatgg acagctcccg ggcggtcggc atccatccgc ggatggtgct ctgcacatgc
     6421 gacaggcggt tcagctgttc gggctgcggc tgcggccagg cggtatcgga actggtttgc
     6481 gcattcagcc gcaactccac cttgccccgt cggagcgacg ccgtcagcaa gtcccgcaag
     6541 gcgggttcga gtgcccgcag ttcgtcgggc agccgcagac tcaggtcgag aaaacgggcg
     6601 ttgaccgatc gcagttcgac ggtcacgctg gttcgcgccg gggtgacggg acgggcgacg
     6661 ggatggtccg aattggccgt ggaagccggt gcttctggag tgcctgcggt ggcgctcgcg
     6721 tagccggtca tgctgtagac tggcatttag atttcaccct gtgcaaacac ccgattatgt
     6781 caaagctgaa gcctgctccg ttgccgtcag gcaccgtggt cggcggctac cagatcgtca
     6841 agaagctggc ggctggtgga tttggtgtgg tgtacctcgc cgaagacgcg cagcggcaca
     6901 tggtggcggt caaggagtac cttccatcgt cgctggctga acggtctcag ggtgaactga
     6961 ccccacgggt caagcccgaa aagcagccgc tgtaccgcct cggtctgaag agcttcttcg
     7021 aggaaggccg gtcgctcgcg cagatctccc acccgagcgt ggtctcggtg ctgaacttct
     7081 ttcgcgagaa cgaaaccgtc tacatggtga tgaactacct gcagggcgag acgctgcagg
     7141 atttcatcgt caccgcgcgc gacctgaagc gcgacaaggt gttccgcgag tcgaccatcc
     7201 ggtcgctgtt cgacgagatc ctgcgtggct tgcgtatcgt gcatcagcac aagatgctgc
     7261 acctcgacat caagcccgcc aacatcttca tcaccaatga caaccgtgcg gtgatgctcg
     7321 acttcggcgc ggcgcgcgag gtgctgagca aggagggcaa tttcatccgc ccgatgtaca
     7381 cgccgggatt cgctgcgccc gagatgtatc gccgagacgg cacgctgggg ccgtggaccg
     7441 acatctacgc gatcggcgcg tgcatctatg catgcatgca gggctatccg ccgaacgatt
     7501 ctccgcagcg catcgagaag gaccgcctgg cgctcagtct gtcgcgcctg cgcaatgtct
     7561 attcggacaa cctgctcgaa gtgaccgaat ggtgcatgtc tctcgacccg ctctcgcgtc
     7621 cgcagagcgt gttcgcattg cagaaggaac tggcgcgcga gacggagcgt cgctacacga
     7681 agttgacctt cggcgagcgc ctgaagctgc aactggagaa cctgaccacc ggcgcgaaag
     7741 cgtgaggttc cggttccgcg tctgcatggc tgagatttcc tgatgcgctt ttccatttat
     7801 caagtcagcc gcaagggcgg tcgcgagaag aacgaggacc ggatgggtta ctcgtacacc
     7861 cgcgattcgg gactgttcgc cctggctgac ggcatgggcg gccaccccga aggcgaggtg
     7921 gcgtcgcaac tcgcgctgca gacgatgtct gccttgttcc agcaagcgtc caagccgacg
     7981 atccccgacc cgctggcatt cctcaaccag gcgatcctgg ccggtcacca gcaactgctg
     8041 cgttactcgg cgcagaaggc cctgagcgac acgcccagaa ccacggtggt ggcgtgcctg
     8101 cttcaaggtg attcggtcta ctgggcgcac tgcggtgatt cgcgtctgta catggtgcgt
     8161 gcgggcaagc tgatcgcgcg cacgcgtgat cactcgtatt ccgaactcca ggatgcgctg
     8221 cattcggtgg tgccgatgcg cgagcggctg aaccgcaatg tgcttttcac ctgcctgggc
     8281 agcccgggca agccggtgat cgacaccgcg ggcccgttca cgctgcaagg tggcgaccgg
     8341 ctgatgctgt gctccgacgg tttgtggggc accgtgaccg acgaatcgat caccgaagaa
     8401 ctgggccgcc gctcgatcgc cgaagccgtg ccggaactcg tcgagcaggc gctgcgcaac
     8461 ggcggaccga agtgcgacaa cgtctcggtg atggctgtcg agtgggagtg ctccgaagcc
     8521 gagcagggcg gctccggcgt gtcgaccgag gaactcggcg atgacgtctt cgcctcgacc
     8581 atccaggcca gcgtgctcgg tcacgacgcg gccgatgacc tcgatgacgc cgagatcgag
     8641 cgctcgatca aggaaatcaa cgacgccatc cggcgctcgt cccagaagaa aaagaactga
     8701 aggagcccat catgggtttt gaacgcacgc agggccggag cgtcgattcg ctgcggccgg
     8761 tccgcatttc gcggcattac acgaagcatg ccgagggttc ggtgctggtc gagttcggcg
     8821 acacgaaggt gctgtgcacc gcctccgtcg aagagaaggt gcccccacac cagcggggct
     8881 cgggtcgcgg ctgggtcacc gccgagtacg gcatgcttcc ccgagccacg cacacccgca
     8941 gcgatcgcga ggcggccaag ggcaagcaga gcggtcgcac gcaggagatt cagcgcctca
     9001 tcggccgttc gttgcgctgc gtgttcgatc tgtcgctgct cggcgagcgc acgatctcgc
     9061 tcgattgcga tgtgatccag gccgatggcg gaacgcgtac ggcggcgatc accggagcat
     9121 tcgtggcggc gcacgatgcc gtgagcgggt tgatcgcgca aagcaagctc ggcgcctcgc
     9181 cgatccgcga tttcgtcgcg gcggtgtcgg tcgggatcgt cgacggcgtg ccgctgctcg
     9241 acctcgaata catcgaggac tcggcctgcg acaccgacat gaacgtggtg atgaccggct
     9301 cgggcggctt cgtcgaagtg cagggcaccg ccgagggcgc atcgttctcg cgcgacgaga
     9361 tgaacagcct gctgcaactg gcagatcgcg gcatccgcga tctggtcgcg gcgcagcgtc
     9421 gagcactcgg gttgtgaagc ggtgaagccg tgaggctggt cctggcgtcc aacaacgcgg
     9481 gcaagctcgc cgaacttcag gcgctgttcc agccgctggg actcgatctg gtgcggcagg
     9541 gcgagctcgg cattgccgag gccgaggagc cgcacctcac cttcatcgag aacgcgctgg
     9601 caaaggcacg ccatgcggct cagcaggcgc gctgtgccgc gatcgccgac gattcggggc
     9661 tctgcgtcga tgtgctcggc ggtgcgccag gtgttcgttc ggcgcgctat gccgaggact
     9721 tcgggcaacc caagggtgat gccagcaaca accgcgtgct tctcgaacag ctggccggcg
     9781 tcacgtccga ggtcgaacgc gttgcgcgct tcgtctgcgc gctggtggcg gtgcggtcgg
     9841 ccgacgatcc gcagccgctg gttgccatgg gccgttggtc cggggtgatc ctgccggcgc
     9901 ctcgtggtga ggccggcttc ggctacgacc cgctgatgtt catggtcgag ttgcagcgct
     9961 cggtggccga actcagcccg tccgagaaga acgggcgcag ccaccgtggt atcgcctcgc
    10021 gcgagatgct gcggcagatg cgcgaggtct ggggccttgg ctgacattcc gatcgtggct
    10081 gcgggaagcg ccgagatcgc atcggcggat gtgaacgtgg agccggcgca gctgctggcc
    10141 cgctacctgc gccctgggac gctgaacctc gccgcgctgc caccgatgtc gttgtatgtg
    10201 cacctgccgt ggtgcctgaa aaagtgccct tactgcgact tcaactcgca tgagcaccgt
    10261 tcctcggcag ccaacgaact gcccgaggca cgctatctgg acgcgttgcg ctgcgacctc
    10321 gaggcggcgc tgcccttcat ctgggggcgc cggatccaca cggtcttcat cggcggtggc
    10381 acgccgagcc tgttcacgcc ggacagcatc gggcagctga ttgccgacat ccgtgcccgg
    10441 ctgcctctgg aacctggctg cgagatcacg ctggaggcca acccgggcac gttcgagcgc
    10501 gatcggtttc gtgcctaccg ggccgcaggc gtcacgcgtc tgtcgatcgg cgtgcagagc
    10561 ttcgacgatg ccaagctcgc ggcgctcggc cgcgtccacg acagcgcgca ggcgagggcg
    10621 gcggtggccg aggcgagcga ggccttcgag accttcaacc tcgacctgat gtacgcactg
    10681 cccggccaga cgctgcccga gctggaagcc gacatcgcga cggcgctgtc gttcaagccg
    10741 ccgcacctgt cgatctacca cctgacgatc gagccgaaca cgctgttcgc caagcacccg
    10801 cctgcgggac ggctggcgct gcccgacgac gacatcgcgt tcgacatgct cgacgtcatc
    10861 acgcagcgct ccgaggacgc cgggctgtcc cgctacgaag tctccgcctt tgctcgagcc
    10921 gcacaccgct gcgcgcacaa cctgaactac tggcagttcg gcgactacct ggggatcggc
    10981 gccggagcgc acagcaagat cagctacccg caccgcgtgg tgcggcaggt gcgcttccgc
    11041 gatccgggca gctacatgtc acgtgcccta gcaggccagg cggtgtcgca ggacgaagaa
    11101 gtgccgcgca agcagctgcc gttcgagttc atgctcaacg gcctgcgtct gaaggaaggc
    11161 atcgaactcg cgcgctacac cgaacgcacc gggctgccgc tcagttcgat cgagcctcag
    11221 ttggccgaag cgacgcagcg ggggctgctg gtgcgcgagc cgaaccggat ccagcccacc
    11281 ccacacggat acgatttcct caacgacctg caggagctgt tcctgccggc ctcttgagta
    11341 cccggggcgg ctgcgcgatc agacgcgatt catcgacatg cccgcggtgc tgcgcagccg
    11401 ttcgatgagc ttcggtgcga tctgggtgag cgggagcact tcgttggcag cgcccgcggc
    11461 gatggcctcg cgcggcatgc cgaacacgac gcaactggct tcgtcctgca ccaggttgta
    11521 gctgccggcg tcgcgcattt ccctcatcgc ggtggcgccg tcggcgccca tgcccgtcag
    11581 catcagcccg agcgcgttcg gtccgaccac gcgggcggcc gacttgaaca gcacctcgac
    11641 cgacggcttg tgccggttga ctggctcgcc gtcctgcaca cgggcgatgt agttggcgcc
    11701 gctgcgctcg acactcaggt gaaagccgcc gggcgcgatg taggcgtggc cgggcaggat
    11761 gcggtcaccg tcgcgtgcct cggccacgcg gaccttgcac agtccgtcga ggcgcgcggc
    11821 gtagctcttg gtgaagccgg gcggcatgtg ctgcgtgatc acgaccgccg ggcagtccgc
    11881 cggcaggttc accagcactt ccttggtggc ttcggtgccg ccggtgctcg cgccgatgaa
    11941 gatgatcttt tcggtcgaca ggcggccgat ggcgctgggt ggcgaactgg gtcgcggcgc
    12001 cgctcccacc gctggcgcgg cggcgggtcg gcgcagatgc gctttcgctg caatgcgtac
    12061 cttgtcggtg atgtcgtggg ccagcagctt cagaccgtcg gcgaccccga tcttgggttt
    12121 ggcaacgaag tcgatcgcgc cgagctccag cgctcgcaag gtgacatcgg cgccgcgctc
    12181 ggtcagcgtc gacaccatga ccaccggcat cggtcgcagc cgcatcagct tgcccaggaa
    12241 gtcgatgccg tccatgcgcg gcatctcgac gtccagggtg atcacatcag ggttcagctc
    12301 gcggatcatc tcgcgcgcta tgtaggggtc tgcagccgca ccgacgcact ccatgtcggg
    12361 ctgcttgttg atgatctcgg tcaacagccc gcgcaccagc gcagagtcgt ccaccaccac
    12421 cacacgggtc tttgccatgg tttgcgctgc cgtatcaaca ggtattcaga ggttgttcag
    12481 aagagatcca ccgagccgcc cgaactggtc gacttgaccg cacgttcgac agccaccttg
    12541 tcctgcgcca tcagcgcgtc ggtgctcgtc ggagccagtc gcttgaccat cgccttgccg
    12601 ctcgcaggca ggaagcagac ctttcgggga tagatgtcga gcacgtcctt cgacaccacg
    12661 gtgatgcgtt cggtcttcag gtaatcgagc acgaaggtcg tgttgcgctc gccgacattc
    12721 agcgagctca tcccgctgat cacggcgccg cccccgaaca ccttggcttc catgcttgat
    12781 ctcgaggcgc ccagcttgag catctcgttg atcagcaatt ccatcgcgta ggaaccgtag
    12841 cgacccgagt ctccgacccc gtcgggcagc atgaagtggt tcatgccgcc gaccctggcg
    12901 ttgcggtccc acaggcatgc ggcgatgcac gagcccagcg ttgtcatgat gagcaggtct
    12961 tcgttgtcga cgtagtactc gccgggaagc accttcacgg catcgctctt gaagtgggct
    13021 tcgtaaaaga agaaggacgc ctggccgggc ttcggggtct gcgcacgcag gcgcgccagc
    13081 cgggagcccg gagctggctg gccgatgccc ggggtcgagc tgccacccgc aagcggcgag
    13141 ggtcgcagcc tcgatggcga agaggctgcg aatgtgttct ggccgatcgg catgttcatg
    13201 ggcagggttc tcagacgcgc tcgtagatcg tcttgccgcg cagccggaac agatgccgcg
    13261 cctcggtgaa attctcggag tggccgacgt acagcagtcc gtgcggcttc atcgtcgcgt
    13321 ggatcttctc gagcaccttg cgctgggtgg cggcgtcgaa atagatcatc acgttgcgac
    13381 agaacacgat gtcgaacgga tcgcccagcg accagcgcgc gtccatcagg ttctgcacgc
    13441 ggaactcgat catgcgggcc agttccggct tcacgcggat gaggccggac tgcgcgccct
    13501 tgccgcgcag gaagtgctgt cgcagccgct cgggcgacaa cccccgcgaa tccagcggat
    13561 acacgccgct gcctgccttg gccagcacgt tggtgtcgat gtcgctggcg atgatccgcg
    13621 tgccgttgtt cgcgcccagg gcctcggtca ccgtcatcgc gatggagtag ggctcctcgc
    13681 cggtcgaagc cgcgctgcac cagatgcgga tgccgccgct ggcgcgcgct cgcaggtcat
    13741 cgacgagtgc cgtgaagtga tggtcctcgc ggaagaacga ggtcaggttg gtcgtcaggc
    13801 agttgatgaa ttcctgccat tcggtgtcgg ccgacggccc gctcgcctgc tccagccact
    13861 ggagatagct gacgaacgat ggatgcccgg tttcgcgcag acggcgggag acccggctgt
    13921 agaccatcgc ctgcttgccg tcgccgaggc tgatcccggc tcgctggtag atgagccggc
    13981 gtacccgatc gaaatcgttc ctgtcgaagg gcaggtcgcg ctcggtgggg tccacgaagg
    14041 agtcggaacg ggtctgaact gtggcggtca tcacggcgga catcttcaaa ctctccggtc
    14101 ggtcggggca tgcccagccc gaaaccgtgg tctcgatgcc tgggtgcata ccggtgatat
    14161 cggccgatcg gcgtgcggct tgagttgaaa ccgtgccggc atctcgctgc cggccagcgg
    14221 aactgaaggg tgtttggatg tctttcgctc cgacatcgcc tggtgccctc ccgctagact
    14281 tggtgcatgt ccgacctccg tctgcactga cttgcccatg tccaccgtgg agctctctcc
    14341 tgccgacctg cagcttcagg tggccctgca gttgctgaag caggcgggtc gcgaggtcag
    14401 cgtcgacgct cccgcggggt ccacgccgtg gctgcaggcg gtggtcgatg cgctgtgcga
    14461 actgtccagc cgcgacgcgc tgacggggct cgccaaccgc cggcagttcg aggtcaccat
    14521 cgcccgggag atcgaccgtg tcgcacgtgc cggtgaaccg gcgctggtgc tgatggccga
    14581 catcgaccat ttcaaggcgg tcaacgacac ctatggccac ccggctggcg atcaggtcct
    14641 gaaagccgtg gcggctgcgc tgcaggattg cgtgcgtccg atggacaccg tcgcccgcta
    14701 tggcggtgaa gagttcgcga tcatccttcc caactgcccg ccggccttcg ggcatctggt
    14761 ggccgagcgg atacgcagca aggtcggccg tcgcccggtc aatgtggcgc ccggcgtcga
    14821 tgtgccggtc acgatcagca tcggaggcgc tttcgccccg caatgggtgc gttcgtccgc
    14881 cccgctgtgg atcgagcgcg ccgatcggca gctctaccgt gccaaggctg aaggccgcaa
    14941 ccgcgtctgt ctggagccgg ctgcggtgtg tcaggtcagc gccgaggaga agggcctgct
    15001 tttcggcact tcccagttcc aggatctcga atgactgccc cgacatgact gccccaaacg
    15061 aatccaccgc ccgacccagc ccgcctgccg gtggtcggtg tgcgcgcacg atggcagtga
    15121 ccagtggcaa gggtggtgtg ggcaagacct tcatctccgc caacctggct gccgcgctgt
    15181 cgcgccgcgg cgaacgcgtt ctggtgctcg atgccgacct cgggctggcc aacctcgatg
    15241 tggtgctgaa cctgcacccg aagatcacgc tccatgacgt gttcaccggc aaggccgaac
    15301 tcgaagaggc gatcctgccc gcgccgggtg gcttttcggt gctgctggcg ggctcgggca
    15361 tggtcgagta ctcccgtctg accagcgagg tccgggatca gctgctggcc atcatcgaca
    15421 aggtcaagcc gcgcttcgac tacatccttc tcgacaccgg cgccggcatt tccgatgtcg
    15481 tgctgttcgc cgtgtcgctg gccgatcagg tgctggttgt cgccacgccc gagccgacgt
    15541 cgatgaccga cgcctacgcc accatcaagg tgctcgcgac gcagcagtcg cggcgcgcga
    15601 tcaagctcgt cgtcaaccag gtcggtcgcc tcggcgaagg ccgcacgatc cgtggtcagc
    15661 tgcagcaggt ggtcgaccgc tacgtgtcgc cgctgctcgg cgggccgtcc gaagcgctga
    15721 agctcgaact cgtcggcgaa gtgccgaccg atacggcggt gcgcgaggcg atcctgcgca
    15781 ggcagttgct gatggatatg ctgccgggtt gcgcggccgc caaggccatc ggcctgctgg
    15841 ccgacaagct gctcgcgtga gcgctgattc aggcgacgcc gcggcgtcaa cgccattcga
    15901 actgctgggc ggagaacctg cggtgcgaca gcttgtcgac cgcttctacg acctgatgga
    15961 cctggagcct gcgtacgcca cggtgcgtgc gctgcatccc gaggtgctcg acggctcccg
    16021 cgataagctg tactggttcc tttgcggctg gctcggtggg ccgcagctct atgtcgagaa
    16081 attcgggcat ccgcggctgc gggcgcggca catgccgtac tcgatcggca tcgccgagcg
    16141 cgatcagtgg atggattgca tgaaacaggc gatgcgtgaa tcgcgcatcg acgagacgat
    16201 ggcggcgcgg ctgaccgaat cgttcttcgg cacggccgac tggatgcgaa accgaggcgt
    16261 ctgaaggctc tgcctcagcc cttcctggcg cgcgacgatc ggtgtggccc agggtcgttt
    16321 gccgcggggg gcatcggctg cagggtgggg cccgtcggtc gcagcagcac cagcagggcg
    16381 ccgtgcccac catcggcagc tcgggcctgc gcaaaggcga tgacctcgct tttctgcacc
    16441 agccaggtct tgaccttcga cttcaagacg ggttcgcggc ccggcgaacc gttgcccttg
    16501 ccgtggatca cccgaacgca gcgcagtccg tgtcgcaccg cttcgcgcag aaaggccgcc
    16561 aggctctcgc gagcctcgtc ccgcctgagg ccgtgcaggt cgagttgcgc ctggatcgac
    16621 cacacaccgc gccgcagctt gcgcacgacc tccgggccga taccctcacc acgccaggac
    16681 agagcatcgt cggtctcgag cagagactcg acatcgaact cgtccgagat cgcctcgcgc
    16741 agcaccgccg cctcgtcgcg ggcccgctgg cgcggcagcg gttcggcgcg tggccgctcg
    16801 agcggaatca cgactccgcg acgcagcggc tgaaccggcc cgacggtgag cgcgaacaga
    16861 tcgcgctcgc gctgcacgcg tgccgcctcg gcgcgggctt ccgcaacacg gcgtgccgac
    16921 tcggccatgg ccttcttcag tgcagtcagg tcctgcagac tgcgcagctt gcggggcggc
    16981 ctcacaacag cccccgttcc gcgaacgaca ccacagcggc acggccgacg atcagatgat
    17041 cgagcacccg cacgtcgacc agccgcagcg cgctctgcag cgtctgtgtc aggaattcgt
    17101 cggcacgcga cggttcggcc acgcccgacg ggtggttgtg cgcgaggatc acggcgctgg
    17161 cctgcagatc gagcgcgcgc ttgaccactt cgcgcgggta gacgctggtc tgggtcagcg
    17221 tgccgcggaa catttcctcg aggcggatca accggttctg cacgtcgagg aacaggaccg
    17281 caaacacctc gtggccgatg tgcgacagct gcaactgcaa gtagtccttg acctgctgcg
    17341 gcgacgacag caccgttcgc tgcgccagtt cctgcgaaag cgatcggcgg gcgagttcga
    17401 tcacggcgct gatctcggct cgtttggcgg ggcccaggcc cttgacccgc ttcaggtcca
    17461 gcgggctggc ctgcagcagg ccggtcacgc cgccgaacgc gtcgagcagt tgctgcgcca
    17521 gttgcaatac cgaagtgccg cgcaggccgg tgcgcagcag cagcgcgatc aactcggcat
    17581 ccgccagcgc ggccgggccg agcgtgagca gcttttctcg gggtcgcgct gcagccggaa
    17641 tgtctttgag cacgatgaag acctgctgtg ttgtgtcgcc gccagcccgc aagcaggccc
    17701 tgcgtgccta cgacgttgtg ccggctcgta aaattgagtc cagtctatcc aaccgggcgc
    17761 gcccttgaac tctgtcattc cggggtcttt cctcactctg cactaccgcc tggccgggcc
    17821 cgatggtgcg gacatcatca acaccttcgc cgacaaggct gccactttgt cgctgggaac
    17881 gggtgaactg gcgccagcga tggaagcccg gctgatcggc atggccgaag gcacgcgcgc
    17941 caccttcgaa ctggctgcgg gcgaggcctt tggcgagcgc aatccagagc tgatgcaacg
    18001 cgtcaagcgc gcgctgctcg acgagctcgg cgaccccgac gagcaataca gcatcggcga
    18061 cgtggtgcag ttcccgaccc cggacggcca gggtgcctac gcgggcgtcg tgcgcgaggt
    18121 cggcggcgac tgggtgcttt tcgacttcaa ccacccgctt gccagccagc cggtgacttt
    18181 cgaggtgcac gtgatcggag tgctgtgatg accaagcccg tccatgtcga ggaagtgttg
    18241 ctggccgagc cgcgaggctt ctgcgccggt gtcgatcgcg ccatcgagat cgtggagcga
    18301 gcgttgacga agttcggcgc gccgatctac gtgcgccacg agatcgtgca caacacctac
    18361 gtggtgaacg acctgcgcgc caagggcgcc atcttcatcg aggagctcgc cgaggtaccc
    18421 gccggcgcga cgttggtgtt cagtgcccac ggcgtgagcc aggcggtgcg ccaggaggcc
    18481 gagcgtcgcg ggttctcgat cttcgacgcc acttgtccgc tggtgaccaa ggtgcacgtg
    18541 gaggtggcca agctgcacaa ggaaggctac gagttcctga tgatcgggca caagggccac
    18601 ccggaagtcg aaggcacgat gggccagctc tacgacggca tctacctcgt cgaggacgcg
    18661 gcggacgtgc agaccgcgcc cgtgaagaac cccgaccggc tggccgtggt gacgcagacc
    18721 acgctgtcgg tggacgacgc ggcgcaggtg ctcgcggccg tgaagcagcg cttcccgaat
    18781 gtgcgcgagc ccaagcagca ggacatctgc tacgccaccc agaaccggca ggacgccgtg
    18841 aaggtgctca ccccgcaggt ggacgtgggg ctgccgacga cgatcaccac gtccacctgc
    18901 ggggtgagca ccagcaatcg cctgcgcgag ctggcgg
//
LOCUS       DAOORV010000068        18784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3582_length_18784_cov_9.093812, whole genome shotgun sequence.
ACCESSION   DAOORV010000068 DAOORV010000000
VERSION     DAOORV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_18784_cov_9.093812"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PKV56_10475"
     CDS             complement(<1..1072)
                     /locus_tag="PKV56_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOX68281.1"
                     /translation="MKPGVTGGNIGGNLSTSGSYPQMPLTDTAIRKAKPGDKAIRMFD
                     ERGLYLEIAPGGGKWWRFKFRFDGKEKLLSMGTFPDISLKEARQRRDEARKLIAEGSD
                     PSAQRKAVRHARKVATLNTVEATARAWLEHRGSAWVERTRTIALASLENDVFPVIGDR
                     PINDIQPADIRKLVQGIEARGAGETAGRVFQRLKAIFRYAITHDLIGTDPTYPLKPAE
                     IFKPRKVQHRAALSERDMPIFLQRLAAYEGDPTTSAALTLLMLTALRPGELRGARWDE
                     IDIDRALWRVPAARMKMKTEHTVPLSKQAIDVIESMRPISGRGALVLPSPFYPGKPLS
                     DGTLNSALARMGYKGLATAHGFR"
     gene            complement(1169..1243)
                     /locus_tag="PKV56_10480"
     tRNA            complement(1169..1243)
                     /locus_tag="PKV56_10480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1208..1210),aa:Arg,seq:cct)
     gene            complement(1314..5786)
                     /locus_tag="PKV56_10485"
     CDS             complement(1314..5786)
                     /locus_tag="PKV56_10485"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOX68282.1"
                     /translation="MSEDALPDPAPAQTPAATRRWPRVLGLTIGLGLLSIALLVGAAA
                     GGLWWALRSEAGSAWVVARLPGVEVTGPRGTLLGDFEAARVEVTLGGGTTRLRLSGFG
                     WRGLQLQRSETAGLWARIAVAEMYARRLDLLLTASETKSPLTAPAALDLPVQIDIAAL
                     RIDALHAQALGDQPLRDLRARVQLGADRGSTHRVDELSAAWDRLQALGAARIETHAPF
                     ALEAQLDLSQVGAAGPSAAASGIDLSAWQATLALAGPLEKPVLRATVRSPATSAHPAQ
                     SLDAQAGLRPFADWPLADLQASTRALDLSALSASAPVTSLSGTATVQTEALDRPAAAR
                     IELSNDAAGRWNDGRLPLRRLSAELVGRPNDRSAFELKRFDAELGTREKSAGHLEGRG
                     QWSAARSTLSATLRALQPSLLDARAPEMLVSGPLSMVASTPPVAASPTAAGAARTPEQ
                     TQAASAAAAGPQLEVQLDLNGTLAQSIGKDRLQTAGKTVQLKLDARLAPQRIEVREAR
                     ASTGGASASLTGAATRASPSAAWQLRGQAALVDFDPLPWWPGREDSPWRRGPHRVNAN
                     GDFDVLLPADLAAPLTALRGNARLAIVDSVLAGTPLEGEATLRSIDNGRADAMLTLQV
                     AGNSLKAQGQFTTAIGTDPGTGPGTGRSDHWDVTLDAPTLSAFAPLWRLLRPAGSDST
                     LAGALTASASVDGRWPELSARGEVAGSNLHVASTTAQQAKASWKFGSTDGAPIDIEAS
                     VSKWRLVGPAASSQALATTPKPAGSAARTARAESPGLESAQVSVKGTVGEHEIELRAS
                     SKALPPAWTDALQAAIGTEPPAPVAVDSSGARSSLIVHARGGATDAPGQRLAGWRGSV
                     QRIELKASQPQARPWIAVKDVGLEARWAGGPTQVTVQPGSADVLGAAVRWKRLAWQAE
                     VPSSSTAGSAAAQPAQIDAQIAFDPLPIAPLLDRLQPNFGWGGDLLLGGRLDLHSDGG
                     LVADIVLERTRGDLTVTDEIGTQSLGLTALRLALSAQDGVWTFSPYAAGRTLGVLAGA
                     VIARTSPEALWPPPEAPIQGAMELNVANLGAWGTWIPAGWRLGGQMKASALFSGRFGA
                     PEVNGDIQGSALSVRNFLEGVNVSDGEVSISLRGDNARIERFSARGGSGSISIEGGAA
                     LGATPSARLQLKAERFVLLGRVDRRIVTSGSAQLGFSADALSVTGAFSVDEGLIDFTR
                     SDAPALSEDVQVVRAAASSAAGNARNRQRPQPVEALPTDAAGSDPAVAATPRNLALNL
                     KIDLGSQLRLRGRGIETGLRGELRLTAPGGRLAVDGTVRATNGTYAAYGQKLTIDRGL
                     ITFNGPPENPRLNIEATRPNTDIRVGVQISGTAQNPRIRLFSDPEVSDVDKLSWLALG
                     RASDGLGSADTALLQTAALALLAGEGDGITEQFTKAIGLDELTLKQADGEVRETVISL
                     GKQLSRRWYVGYERGLNATEGSWQLIYRIARRFTLRAQSGFENSLDLIWTWRWG"
     gene            complement(5783..7720)
                     /locus_tag="PKV56_10490"
     CDS             complement(5783..7720)
                     /locus_tag="PKV56_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOX68283.1"
                     /translation="MRWRQATAAGACVLALMGLTGCASVAQFFKADDAPSAEQPPRPV
                     VRVYRLEVDAPGELRTLLDTYLDLARFQSAPESEDITPAEVDRLIAAAPAQVRSLLET
                     EGYFNPEVQVGRVGLQAGSAAARAAARERAEDSPENEPATGPREIPLLRVSVKPGPRA
                     VVDRFTFKAQGPLQQAAEGGSLDARVLMAQVRNTWRLPTELPFRQSDWSSAKNGALAK
                     LRADGYPLATLSESAARVDAQANTVQLDVTADSGVLYRLGPLSIRGLNRYDEAAVRAL
                     SMLEPGAPYRESQLIDFQERLQKAGLFEGASVVLDVDPATSQAAPINVQLRELPMQQA
                     TFGVGYSANTGPRVTVEHTHHQPFGWRWIDKNKVELGPDLKAWHGELTSYPLEGLYRN
                     LIAGSAEQLKTIDEVRTSWTARIGRTQDTPRIERLYYAELTHSRLETMSDTGTTTAVN
                     DAVSGNYNWVYRDLDNALLPTEGYTLNAQAALGYARSGNENNGPFGRLYGRYTGYRPL
                     GNAWYATARVEAGQVLANNAVGIPDTLLFRAGGDDSVRGYAYRSLGPKVDGALLSGRV
                     LLTGSAEIARPISPRMPALWGAAFIDAGNSADHWQDLHPVLGYGVGLRWRSPVGPLRM
                     DVAYGQSVQKFRMHLSVGIAF"
     gene            complement(7717..8910)
                     /locus_tag="PKV56_10495"
     CDS             complement(7717..8910)
                     /locus_tag="PKV56_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX68284.1"
                     /translation="MLIDFFLSLRAAKLKVSVREYLTLLEALQAGVLDDDEVHGGPTL
                     DGFYVLARATLIKDEANFDAFDRAFGAYFKGVETLTDFAQDIPLEWLRKVLELELSPE
                     EKAAIEALGWDELMETLKKRFEEQKERHQGGSKWIGTGGTSPFGAYGVNPQGIRIGQD
                     RSRQRSAVKVWDQRRFKDYDADTELGTRHIQVALRKLRRFAREGSADELDLDDTIRAT
                     AANAGHLDLKMIPERHNHIKVLLLMDVGGTMDDHVHRVEEMFSAAKSAFKHLEFYYFH
                     NCVYDHMWKNNQRRRSEKFETRDILRKYNRDYKLIFVGDATMSPHEILQPGGSVEYHN
                     EEAGAEWLQRLTQAFPKFAWINPEPQALWSYRQSIALIQQLMNQRMYPLTLPGLDAAM
                     RTLGR"
     gene            complement(8904..9500)
                     /locus_tag="PKV56_10500"
     CDS             complement(8904..9500)
                     /locus_tag="PKV56_10500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOX68285.1"
                     /translation="MGSMTEPIELRGTHANLVPLSPTHASALAEASRDGELWKLWYTS
                     VPSPDGMAAEISRRLSLQAAGSMLPFSVLDAGGTPVGMTSFMHIDARHRRVEIGSTWY
                     AARVQRTALNTDCKRMLLAHAFDSLDCIAVELRTHRLNTRSRRAIERIGAQLDGILRA
                     HQRLPDGTLRDTAVYSITASEWPAVRAHLQGLLERPGC"
     gene            complement(9517..10371)
                     /locus_tag="PKV56_10505"
     CDS             complement(9517..10371)
                     /locus_tag="PKV56_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX68286.1"
                     /translation="MKFKGTQDYVATDDLMLAVNAAATLKRPLLVKGEPGTGKTMLAE
                     EVAKSLGMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLADVDGGAKVRDIRNYIVQG
                     VLWKAFTADEPVVLLIDEIDKADIEFPNDLLREIDRMEFYVYETRELIRAVHRPLVFI
                     TSNNEKELPDAFLRRCFFHYIQFPDAQTMQRIVQVHFPDLKQNLLAAALQRFYEVRSL
                     PGLKKKPSTSELLDWLKLLVAEDIPLAALQSQDESVAMPPLLGALLKTEQDVSLFEKL
                     VFMQRQRR"
     gene            10455..11117
                     /locus_tag="PKV56_10510"
     CDS             10455..11117
                     /locus_tag="PKV56_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX68287.1"
                     /translation="MRPTMRTLTQLAFWLICCGFSTGTLAQDAKAGEKKSSWCSSCHG
                     VPGFKAISPEVHRVPMIGGQSASYIVSALKAYKYGERLNPTMKGVAATLTDADMADLG
                     AFYGAQVQGGAAAPAGTTAASSRQAADLLKKGNCVSCHGENYSKPIAPAYPKIAGQHA
                     DYLFAALKAYQTDGNPQIGRKNPVMGGVARQFTQDDLKVLANHVGSLQGDLHTVVPSR
                     SR"
     gene            11264..12556
                     /locus_tag="PKV56_10515"
     CDS             11264..12556
                     /locus_tag="PKV56_10515"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOX68288.1"
                     /translation="MTSPALKKIPVDQVRLGMHLHELCGAWLDHPFWKTKFVLEDVAD
                     LKKLQQSGIRECWIDVSKGLDVAATFVDEPALVARRTGAASSALDVDTATDLARAAQI
                     RPPAPVALEDEVHRAADLCNQARAAVISLFSEARMGRVVFSAECEELVHGISDSVMRN
                     TTAMLSLARLKSHDDYTYMHSVAVAALMAALARQLQLEPDEIRMAAKAGLLHDMGKAV
                     MPRDILNKPGKLTDEEFVVIRTHPERGHALLMAGEVADEAVLDVCLHHHEKTDGSGYP
                     HRLPGDRISMFAKMGAVCDVYDAITSNRPYKQGWDPAESIARMAGWAKGHFDAAIFKA
                     FVTSLGIYPTGSLVRLKSGLLAVVTRQNDAALTAPEVKVFFSTKQGLHVPPRLLDLSS
                     PGCTDRIIVRESNEGWKFGHLDELWAGEEALRKIGRKR"
     gene            12622..14217
                     /locus_tag="PKV56_10520"
     CDS             12622..14217
                     /locus_tag="PKV56_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX68289.1"
                     /translation="MNAVGWRRRTIGAVAGLLSTCVLIAGASAQTLRWASQGDPQTMD
                     PHSQNELLTNNLNGQVYESLVNRDRRLDLVPVLATSWQQNGPLRWRFKLRPNVKFHDG
                     TPFTADDVVFSVRRARDSASDIRAYALALGEPQAVDALTVDFVLKDVNPVFLQHLSTL
                     QIMSRVWSEHHGAVKPIDYRSRQEGHAALHANGTGPYKLSLRQPDVKTVYQRNAAWWG
                     TFEGNVQEVVYTPIKSDATRIAALISGELDFVLDPAPQDLPRLRSTPGVKVTEGPENR
                     IVFIGMDQARDQLLYGSVKGTNPFKDLRVRRALYQAIDIETIRSRLMRGQSLPTGGIT
                     PSPVGAYNDPELEKRLPFDLNAARQQMTDAGYPQGFEVTLDCPNNRYINDEEICQTLA
                     AMWARLGVKVRVNAMPRSTYFPKLQRLDTSMYLLGWGGAITDAETTLTPLLRSRGEGG
                     IGAWNFGQFSDARLDQLAQASSREGDPKKREQLIKAALQQQKDQVLYLPLHRQVVPWG
                     ARQNVDLVHRADNWVEFSWIRMR"
     gene            14214..15524
                     /locus_tag="PKV56_10525"
     CDS             14214..15524
                     /locus_tag="PKV56_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOX68290.1"
                     /translation="MTDRFVPAAQSDPRAFLRALYDAAVTRALPAGVMAASLPAPPAG
                     RTVVVGAGKAGGSMAAAVDALWPAGAPLSGLVVTRYDHVPPAYRARPGRIEVVEAAHP
                     VPDAAGLQAARRIVELVQGLGADDLVLCLISGGGSSLLSLPAQGVTLDEKRAINQALL
                     KSGAAIDEMNCVRKHLSAIKGGRLAVMCAPARVVTLLVSDVPGDAPDVIASGPTVADP
                     TTCADALAILQRYGIDLPEAARRGLESGAFETPKPGHPALAGHQVHLIATPQQSLESA
                     AAVARAAGVDAHILSDEIEGESREVGKVHAALARAVARRGAPFARPCVILSGGETTVT
                     VRSGGGRGGRATEFLLGCAIALQGEPGVHVLAADTDGIDGIEDNAGAIVTPDTLIRAQ
                     ALGMKAQDFLDRNDAYNFFAPLGDLVVPGPTYTNVNDFRALLIL"
     gene            complement(15493..15882)
                     /locus_tag="PKV56_10530"
     CDS             complement(15493..15882)
                     /locus_tag="PKV56_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68291.1"
                     /translation="MLIALWIITALSCLLWSLGAWGLHTLLSLDASRIADLKPLIDQL
                     PERLPYAAFIDAWIPGWREMLKFGVDLMQTVLGWIGGAATPVVWTVWALGMLMLLGLA
                     AVATWGIRSLSRQARQLATGSAVPGNR"
     gene            16041..18626
                     /locus_tag="PKV56_10535"
     CDS             16041..18626
                     /locus_tag="PKV56_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX68292.1"
                     /translation="MNTAVESTRRPASLWPLAWRQLARDFRAGELRLLVLAVTLAVAA
                     LTAVGFFADRINGGLARDATQLMGGDAIVASDQPTPQAIVDKARSLGLATATTTAFPS
                     MGRASDERGGASRLVSVKAVSDGYPLRGRLRLSDGAGTPEVSVAQGPRPGTVWVESAL
                     LDALQLRVGDSLLLGDATLRIAQVIVIEPDRGAGFMSFSPRVMLNQADLPATGLIQPA
                     SRVTYRLIVAAPDSKERSDPRVREFTRWTESQLKSQALRGVRVESLEGGRPEMRQTLD
                     RAEKFLNLVALLSALLAAVAVGIASRDFASRRLDDCAMLRVLGQPQRTIALQYLIEFT
                     LVGLLASVAGVALGFVVHHGFVWLLAGLVSTSNLPTASWWPALFGLGVGFTLLMGFGM
                     PPVLQLARVPPLRVIRRDVGALKPASIAVLLAGALGFAALLLAVSSDVKLGLIAVGGF
                     AAAIAMFALLSWAAVLLLRRAVPESRAPRWLVLATRQIAARPAFAVLQVSALSVGLLA
                     LVLLVLLRTDLVSSWRAATPPDAPNRFVINVQAGQGDEFKARLRQAGIAQYDWYPMIR
                     GRLVAINGKTLSADSMTGERARRLVEREFNLSHSSVLPPHNQLVEGRWVPEEDGGLSV
                     ESGLAETLGLKLGDTLRFDIAGQPTQARVTSVRKVDWGSMRVNFFVLFPNSTLEDVPM
                     TYIAAFKTPAAGGAKAAAEFDNALIRLFPNITSIDVSASIAQVQGVLEQVIGAVQFLF
                     VFTLAAGLVVLFAGVSATRDARSREFAVMRALGASGRLLAQVQRVELLGVGALAGVLA
                     SLAALAVGWVLARQVFDFNWSPSPWVPLIGGVTGALLSLAAGWWGLRDVLRRPVVETL
                     RQAAQ"
BASE COUNT         2781 a         6332 c         6474 g         3197 t
ORIGIN      
        1 tgcggaaacc gtgtgccgtg gccaggccct tgtagcccat gcgggccaat gcgctgttca
       61 gcgtgccatc gcttagcggc ttgccaggat agaacgggga gggcagcacc agtgcgcccc
      121 gaccgctgat tggccgcatc gactcgatca cgtcgatggc ctgcttcgac agcggcaccg
      181 tgtgttcggt tttcatcttc atgcgggccg ctggaacgcg ccacagggcg cgatcgatat
      241 cgatctcgtc ccaccgtgcc ccgcgcagtt cgccgggccg cagtgccgtc agcatcaaca
      301 gcgtgagtgc cgctgatgtc gtggggtcgc cctcgtaggc cgcgaggcgc tgcaggaaga
      361 tcggcatgtc gcgttcggac agtgccgccc gatgctgcac cttgcggggc ttgaagattt
      421 cagccggttt cagtggatag gtcggatcgg tcccgatcag gtcgtgcgtg atggcgtatc
      481 ggaagatcgc cttcagtcgc tgaaacaccc ggcccgccgt ttcgcccgcg ccgcgcgctt
      541 cgatgccttg caccagcttg cggatgtcgg cgggttggat gtcgttgatc ggtcggtcgc
      601 cgatgacggg aaacacgtcg ttctccagcg atgccagggc gatggtgcgg gttcgttcga
      661 cccatgcgct gccacgatgc tccagccatg cgcgtgccgt cgcttcaacg gtgttcagag
      721 ttgccacctt gcgggcgtgt ctgactgcct tgcgttgcgc gctggggtcg gagccttcag
      781 caatgagttt tcgggcttcg tcacggcgct ggcgagcctc cttgaggctg atgtctggga
      841 acgtccccat cgagagcagc ttctcttttc cgtcgaaacg aaacttgaat cgccaccact
      901 ttccaccgcc cggcgctatc tcaaggtaca gcccgcgctc gtcgaacatg cggatcgctt
      961 tgtcgccggg cttcgccttg cggattgctg tgtcagtcag tggcatttgg gggtaactcc
     1021 cactcgtaga taagttaccc ccaatgttgc ccccagttac cccaggcttc aagaggaaac
     1081 ttcaggaaca gtcaggaatg aaaaaagccc cagagcgttg atttctctgg ggcttccggt
     1141 ctttctgaga gcctttcggc tttttctatg gtcccgccga caggaatcga acctgtattt
     1201 gccgcttagg aggcggccgt tctatccatt gaactacggc gagctgaacc cggattttca
     1261 cacgcgcttt cagggccggt ccgggctctc ctgcgttgct cactcgggca cgttcatccc
     1321 catcgccagg tccagatcag gtccagcgag ttctcgaatc ccgattgcgc gcgcagcgtg
     1381 aagcgccgcg cgatgcggta gatcagttgc cagctgccct cggtcgcgtt caggccgcgc
     1441 tcgtagccga cgtaccaccg ccgtgacagc tgcttgccca gcgagatgac ggtttctcgc
     1501 acttcgccgt cggcctgttt cagcgtcagt tcgtcgagcc cgatggcctt ggtgaactgc
     1561 tcggtgatgc cgtctccctc gccggccagc agggccagtg cggcggtctg aagcagtgcc
     1621 gtgtcggcgg aacccaggcc gtcgctggcc cggcccagcg ccagccatga cagcttgtcg
     1681 acgtccgaaa cctcggggtc cgaaaacagg cggatgcgcg ggttctgcgc ggtgccgctg
     1741 atctgcacac cgacgcggat gtcggtgttc gggcgggtgg cctcgatgtt cagccgcggg
     1801 ttttccggtg gaccgttgaa ggtgatcagg ccgcggtcga tggtgagttt ctgtccgtag
     1861 gccgcgtagg tgccattcgt ggcacgcacc gtgccgtcaa cggccagtcg accgcctggc
     1921 gcggtgagtc gcaactcgcc acgaagtccg gtttcgatac cgcgcccgcg caggcgcagc
     1981 tgggagccga ggtcgatctt caggttgagc gccaggttgc gcggtgtcgc agcgacggcc
     2041 ggatcggagc ccgcagcgtc tgtgggaagc gcctcgacag gctgcgggcg ctggcggttg
     2101 cgggcgttgc ctgcggcgct gctggctgca gcgcgcacca cctgcacgtc ttcggacagc
     2161 gcgggcgcgt cgctgcgtgt gaagtcgatc agtccctcgt cgaccgagaa ggcaccggtc
     2221 acgctgagcg cgtcggcgct gaaacccagt tgtgccgagc cgctggtgac aattcgccgg
     2281 tcgacgcggc cgagcagcac gaagcgttcg gccttcaatt gcaaccgcgc gctcggtgtg
     2341 gcacccagcg ccgcgccgcc ctcgatgctg atgctgcccg agccgcccct cgcgctgaac
     2401 cgttcgatgc gggcgttgtc gcctcgcagg gagatcgaga cctcgccgtc gctgacgttc
     2461 acgccctcca ggaaattgcg cacgctcagc gcgctgcctt ggatgtcgcc gttgacctcc
     2521 ggtgcgccga agcggcccga gaacagcgcg ctggccttca tctgtccccc gagccgccag
     2581 ccggccggta tccacgtgcc ccaggcgccg aggttggcga cgttcagctc catcgcgccc
     2641 tggatcggcg cttcgggcgg cggccacagg gcctcgggcg aggtgcgcgc gatcaccgcg
     2701 cccgccagca cgccgagcgt gcgtccggcg gcgtaaggcg agaaggtcca gacgccgtcc
     2761 tgcgcgctca gggccagtcg caaggcggtc agcccgaggg actgcgtgcc gatctcgtcg
     2821 gtcaccgtca ggtcgccgcg cgtgcgctcg agcacgatgt cggcgaccag tccgccatcg
     2881 ctgtgcagat cgagccgccc ccccagcaac aaatctcccc cccagccgaa attgggttgg
     2941 agcctgtcca gcagcggtgc gatcggcagc gggtcgaatg cgatctgtgc gtcgatctgt
     3001 gccggctgtg ccgctgcgct gccggcggtg gaggaactcg gcacctcggc ttgccaggcc
     3061 aggcgcttcc agcgaacggc agcgcccagg acatcggcgc tgcccggctg cacggtcacc
     3121 tgcgtcggtc ccccggccca ccgggcctcg aggccgacat ccttcacggc gatccaggga
     3181 cgtgcctgcg gctgacttgc cttcagctcg atgcgctgga cgctgccgcg ccagcccgcc
     3241 aggcgctgtc ccggcgcatc cgttgcacca ccccgtgcat gcacgatcag cgagcttcgc
     3301 gcgccggatg aatcgacggc gaccggagct ggcggctccg tgccgatggc cgcctgcaat
     3361 gcgtcggtcc aggccggtgg cagcgctttc gagctggctc tcaactcgat ctcgtgttcg
     3421 cctaccgtgc ccttcacgga cacctgcgcc gattcaaggc ccggtgattc ggcgcgcgct
     3481 gtccgggccg cggaccctgc cggcttcggt gtcgtggcca gcgcctggct ggatgccgcc
     3541 gggccgacca gccgccactt cgacacgctc gcctcgatgt cgatcggcgc gccgtccgtg
     3601 ctgccgaact tccagcttgc cttggcctgc tgagcggtcg tggacgcaac gtgcaggttg
     3661 ctgccggcca cttcgccccg ggcactgagc tccggccagc gcccatcgac gctggccgac
     3721 gcggtgagcg cgccagccag cgtgctgtcg ctgccggctg gccgcaacag acgccacagg
     3781 ggtgcgaagg ctgacaacgt cggcgcgtcg agcgtcacgt cccagtgatc cgagcggcct
     3841 gtcccggggc cggtgccggg gtctgtgccg atcgcagtgg tgaattgccc ctgcgccttc
     3901 aggctgttgc cggcgacctg caacgtcagc atcgcatccg cgcgaccgtt gtcgatgctg
     3961 cgcaaggtgg cttcgccctc gagtggcgtg cccgccagca cgctgtcgac gatggccagc
     4021 cgggcgttgc cgcgcagggc ggtcagcggt gctgccaggt ccgcaggcag cagcacgtcg
     4081 aaatcgccgt tcgcgttgac gcggtgaggt ccgcggcgcc agggcgaatc ctcgcggccc
     4141 ggccaccacg gcagcgggtc gaagtcgacc agcgcggcct gcccccggag ctgccatgcg
     4201 gccgagggcg atgcgcgggt cgccgcgccc gtcaggctgg cactcgcgcc gccggtgctg
     4261 gcgcgtgcct cgcgcacctc gatgcgttgc ggggccaggc gggcatcgag tttcagctgc
     4321 acggtcttgc cggccgtctg caaccggtcc ttgccgatgg actgcgcaag ggtgccgttc
     4381 agatcgagct gaacctccag ctgggggccg gcggcagcgg ccgaggcggc ttgcgtctgc
     4441 tccggcgtgc gggcggcacc tgcggcggtg ggcgaagcgg caacgggcgg cgtcgacgcc
     4501 accatcgaca gcggcccgct caccagcatt tccggcgccc tggcgtcgag cagcgacggc
     4561 tgcagcgcgc gcagcgtggc actcagcgtc gatcgcgcgg cgctccactg gccccgacct
     4621 tccaggtgac ccgccgactt ctcgcgggtg ccgagttcgg catcgaaccg cttcagctcg
     4681 aaggcgctgc gatcattcgg ccgtccgacc agctctgcgc tcaggcggcg cagcggcagc
     4741 cggccatcgt tccagcgacc cgccgcgtcg ttgctcagct cgatgcgtgc agcggccggc
     4801 cgatcgagcg cctcggtctg caccgtggcg gtgccgctca gcgaagtgac gggtgccgac
     4861 gcgctgagcg cggacagatc gagcgcgcgc gtgctggcct gcagatcggc cagcggccag
     4921 tcagcgaacg gacgcaaccc ggcctgcgca tcgagcgact gggccggatg cgcgctggtg
     4981 gctggcgagc gcacggtcgc gcgcaggacc ggcttctcca gcgggccggc cagcgcgagc
     5041 gtggcctgcc acgccgacag atcgataccc gatgcggcag cagatggccc ggctgcaccg
     5101 acctgggaga gatcgagctg agcctcgagc gcgaagggcg catgcgtctc gatgcgcgct
     5161 gcgccgagtg cctgcaaccg gtcccaggcc gcgctgagtt cgtcgacgcg gtgcgtgctg
     5221 ccgcggtccg cacccagttg cactcgggcc cgcaggtcgc gcagcggctg gtcgccgagc
     5281 gcctgcgcgt gcagcgcatc gatgcgcagc gcggcaatgt cgatctgcac gggcaggtcc
     5341 agcgcggccg gcgctgtcag cggtgacttg gtttcgctcg cggtgagcag cagatcgagc
     5401 cgtctcgcgt acatctcggc cacggcgatg cgggcccaca gcccggctgt ttcgctgcgt
     5461 tgcagctgca atccacgcca gccgaagccg ctgagccgca ggcgggtggt tccgccgccg
     5521 agcgtgacct cgacccgcgc ggcctcgaag tcgccgagca gcgtgccgcg gggccctgtc
     5581 acctcgaccc cgggcagtct ggcgaccacc caggcgcttc cggcctcgct gcgcagcgcc
     5641 caccagagcc cgcctgccgc agcaccgacc agcaaggcaa tcgacagcaa gccgagcccg
     5701 atggttaggc cgagcacgcg cggccagcgg cgggtggccg caggcgtctg ggcgggcgct
     5761 ggatcgggca gggcgtcttc gctcaaaacg cgatccccac gctgaggtgc atgcggaact
     5821 tctgcaccga ctgcccgtag gccacatcca tgcgcaacgg cccgaccggg cttcgccagc
     5881 gcaggccgac accgtagccg agcaccggat gcaggtcctg ccaatggtcg gctgagttac
     5941 cggcgtcgat gaacgccgcg ccccacagcg cgggcatgcg cggagagatc gggcgtgcga
     6001 tctccgcgct gccggtcagc agcacccggc cgctgagcag ggcaccgtcg accttcgggc
     6061 cgagcgaccg gtaggcgtag cccctgaccg aatcgtcacc gccggcccgg aacagcagcg
     6121 tgtcggggat gccgaccgcg ttgttcgcca gcacctgacc ggcctcgaca cgtgcggtgg
     6181 cgtaccaggc attcccgagc gggcggtagc cggtgtagcg cccgtagagg cggccgaacg
     6241 ggccgttgtt ctcgttgccg ctgcgcgcgt aacccagtgc ggcctgtgcg ttgagcgtgt
     6301 agccctcggt gggcagcagc gcattgtcga gatcccggta cacccagttg tagttgcccg
     6361 acacggcgtc gttcaccgcg gtggtggtgc cggtgtccga catggtttcc aggcgtgaat
     6421 gcgtcagttc ggcgtagtac agccgttcga tgcgcggtgt gtcctgggtg cgaccgatgc
     6481 gcgcggtcca ggaggtgcgg acttcgtcga tcgtcttgag ctgctcggcg ctgcccgcga
     6541 tcaggttgcg atacaggccc tccagcgggt acgaggtcag ctcaccgtgc caggccttga
     6601 ggtccgggcc gagttcgacc ttgttcttgt cgatccagcg ccagccgaac ggctgatggt
     6661 gtgtgtgctc caccgtgacg cgggggccgg tgttggcgct gtagccgaca ccgaaggtgg
     6721 cctgctgcat cggcagctcg cgcagctgca cattgatcgg cgccgcctgc gacgtcgcgg
     6781 ggtcgacatc cagcacgacc gaggctcctt cgaacaggcc ggccttctgc agccgttcct
     6841 ggaagtcgat caactgcgac tcgcggtacg gtgcccccgg ctcaagcatc gacagcgctc
     6901 gcacggccgc ttcgtcgtaa cggttcaggc cgcggatgct gaggggcccc aggcgataca
     6961 ggacgccact gtcggccgtc acgtcgagct gaaccgtgtt cgcctgcgcg tcgacgcgcg
     7021 cggccgattc gctcagcgtg gccaacgggt agccgtcggc gcgcagcttg gccagtgccc
     7081 cgttcttggc gctgctccag tccgactggc gaaagggcag ctcggtcggc aggcgccagg
     7141 tgttgcgcac ctgggccatc agcaccctgg cgtcgagcga gccgccctcg gccgcctgct
     7201 gcagcggacc ctgcgccttg aaggtgaacc gatcgacgac ggcgcgcggc ccgggcttga
     7261 cgctcacgcg cagcagcggg atctcgcgcg gccctgtggc gggctcgttc tcgggggaat
     7321 cctcggcacg ctctcgtgcg gcggcccgtg ccgcggccga tccagcctgc aggccgacgc
     7381 gtccgacctg cacttcgggg ttgaagtagc cctcggtttc gagcagcgat cgcacctgcg
     7441 caggcgcggc ggcgatcagg cggtcgacct cggccggggt gatgtcctcg ctctcagggg
     7501 cactctggaa ccgcgcgaga tcgaggtagg tgtcgagcaa tgtgcgcagc tcgccggggg
     7561 catcgacttc gagccggtac acgcggacca ccggacgcgg cggctgctcg gcggagggcg
     7621 cgtcgtccgc cttgaagaac tgcgccacgc tcgcgcagcc ggtcaggccc atcagggcga
     7681 ggacgcaggc gccagcggcc gtcgcctggc gccacctcat cggccgagcg tgcgcatggc
     7741 cgcatcgagt ccgggcagcg tcagcgggta catgcgctgg ttcatcagtt gctggatcag
     7801 cgcgatgctc tgccggtagc tccacagcgc ctgcggctcc gggttgatcc aggcgaactt
     7861 cgggaacgcc tgggtcagcc tctgcagcca ttcggcgccg gcttcctcgt tgtgatactc
     7921 cacgctgccg ccgggctgca ggatctcgtg ggggctcatc gtcgcatcgc cgacgaagat
     7981 cagcttgtag tccctgttgt acttgcgcag gatgtctctc gtctcgaatt tctcgctgcg
     8041 ccgacgctgg ttgttcttcc acatgtggtc gtagacgcag ttgtggaagt aatagaactc
     8101 gaggtgcttg aacgcagact tggcggcact gaacatttcc tcgacccggt gcacgtgatc
     8161 gtccatcgtg ccgcccacgt ccatcagcag cagcaccttg atgtggttgt ggcgctccgg
     8221 gatcatcttc aggtcgaggt ggccggcgtt ggccgcggtc gcgcggatgg tgtcatcgag
     8281 gtcaagctcg tcggccgagc cttctcgggc gaagcgccgc agcttgcgca gcgccacctg
     8341 gatgtgacgc gtgccgagtt cggtgtccgc gtcgtagtcc ttgaagcggc gctggtccca
     8401 gaccttgacc gcgctgcgct ggcggctgcg atcctggccg atccggatgc cctgcggatt
     8461 gacgccgtag gcaccgaacg ggctggtgcc tccggtgccg atccacttgc tgccgccctg
     8521 gtggcgctcc ttctgctctt cgaaccgctt cttcagcgtt tccatcagct cgtcccagcc
     8581 cagcgcctcg atcgcggcct tctcctcggg actcagttcg agctcaagca ccttgcgcag
     8641 ccattcgagc ggaatgtcct gcgcgaaatc ggtcaaagtc tcgacgccct tgaagtacgc
     8701 gccgaatgcg cggtcgaagg catcgaagtt cgcctcgtcc ttgatcagcg tggcgcgcgc
     8761 cagcacgtag aagccatcga gcgtcgggcc accgtggacc tcatcgtcgt cgagcacgcc
     8821 cgcctgcagc gcctcgagca gcgtcaggta ctcgcgcacc gacaccttga gcttcgcggc
     8881 gcgcagcgac aggaagaagt cgatcagcat ccgggtcgct ccagcaggcc ctgcaggtgc
     8941 gcgcgcaccg cgggccattc gctcgcggtg atgctgtaga ccgcggtgtc gcgcagcgtg
     9001 ccgtcgggca gtcgctggtg cgcgcgcagg atgccgtcga gttgcgcacc gatgcgctcg
     9061 atcgcgcgtc ggctgcgggt attcaggcgg tgcgtgcgca actcgaccgc gatgcagtcc
     9121 agcgagtcga acgcatgggc cagcagcatc cgcttgcagt cggtgttcag cgcggtgcgc
     9181 tgcacccggg cggcgtacca ggtcgagccg atctcgactc ggcggtgcct tgcgtcgatg
     9241 tgcatgaagc tggtcatgcc gaccggtgtg ccgcccgcat cgagcacgct gaacggcagc
     9301 atcgagccgg cagcctgcag cgaaagccgt cgggagatct cggcggccat gccgtcgggg
     9361 ctcggcacgc tggtgtacca gagcttccac aactcgccgt cccggctggc ctcggcgagt
     9421 gcggacgcgt gcgtcggcga cagcggcacc aggttcgcgt gcgtgccgcg caactcgatc
     9481 ggctcggtca tcgaacccat gtgtgcgccc gaacgatcaa cggcgctgcc gctgcatgaa
     9541 caccagtttc tcgaagagcg acacgtcctg ctcggtcttc agcaaggcgc ccagcaaggg
     9601 cggcatggcg acggactcat cctggctttg cagggcagcc agcgggatat cctcggccac
     9661 cagcagcttc agccagtcga gcagttcgga ggtggaaggc ttcttcttca gccccggcag
     9721 gctgcgcacc tcgtagaagc gctgcaacgc ggcggcgagc aggttctgct tcagatcggg
     9781 gaaatggacc tggacgatgc gctgcatggt ctgcgcgtcg ggaaactgga tgtaatggaa
     9841 gaagcatcgg cgcaggaagg cgtccggcag ttccttctcg ttgttcgagg tgatgaacac
     9901 cagcggccgg tgcaccgcgc ggatcagctc gcgggtctcg tagacgtaga actccatgcg
     9961 gtcgatctcg cgcagcaggt cgttcggaaa ttcgatgtcg gccttgtcga tctcgtcgat
    10021 cagcaacacc accggctcgt cggcggtgaa cgccttccag agcacgccct gcacgatgta
    10081 gttccggatg tcgcgcacct tggcgccgcc gtcgacatcg gcgagctggc tgtcgcgcag
    10141 ccggctcacc gcgtcgtact cgtacaggcc ctgctgcgcc ttggtggtgc tcttgatgtg
    10201 ccactgcagc agtggcatcc ccagcgactt ggccacttcc tcggccagca tcgtcttgcc
    10261 tgtgcccggt tcacccttca cgagcaaagg gcgtttcagc gtggcggccg cgttgacggc
    10321 cagcatcaaa tcgtcggtgg cgacgtagtc ttgcgttccc ttgaacttca tggcggtgcc
    10381 gggtcgtcga tgccgatgca gtatagagaa cgccctttct atactgcgcc gctgttcctg
    10441 ttcccgaacc aaggatgcgc ccgaccatga gaaccctgac ccagctcgcc ttctggttga
    10501 tctgctgcgg cttcagcacc ggaacgcttg cgcaggacgc caaggccggc gagaagaagt
    10561 cttcctggtg cagcagctgc cacggcgttc cagggttcaa ggcgatctcc cctgaagtcc
    10621 acagggttcc gatgatcggg ggccagagcg cgtcgtacat cgtgtcggca ctgaaggcct
    10681 acaagtacgg cgagcgactc aatccgacga tgaagggcgt cgccgcgacc ctgaccgacg
    10741 cggacatggc cgatctcggc gcgttctacg gggcgcaggt tcagggcggg gcggcggcac
    10801 ccgcgggcac cacggcagca tcatcgcggc aggccgccga tttgctgaag aagggcaact
    10861 gcgtgtcctg ccatggcgag aactacagca agccgatcgc tcctgcctat ccgaagatcg
    10921 ccgggcagca tgccgactat ctcttcgccg ctctcaaggc ctatcagacc gatggcaacc
    10981 cgcaaatcgg gcgcaagaac ccggtcatgg gtggcgtggc gcgtcaattc acgcaggacg
    11041 atctgaaggt ccttgcgaac cacgtcggct cgttgcaggg agatctccac acggtcgtgc
    11101 cgtcccggtc ccgctgatct cgcgccagag gggctgcatt tcggccgttt ttttgccatt
    11161 aagccgctga tccggcggct ttttgcctgc tgtcgctgcc gacccgtccg ccgtgtgaca
    11221 gcatctctca agttcctgcg gttcggctcc gatattgacc gagatgacat cccccgccct
    11281 caagaagatc cccgtcgacc aggtccgact cggcatgcat ctgcacgagc tgtgcggtgc
    11341 ctggctggac caccctttct ggaagacgaa gttcgtgctc gaggacgtgg ccgacctgaa
    11401 gaagctgcag caaagcggca tccgagagtg ctggatcgac gtcagcaagg gcctcgatgt
    11461 cgccgccacg ttcgtcgacg aaccggcgct ggtcgcacgc cggaccggtg ccgcgtcttc
    11521 tgcgctcgat gtcgacacgg cgaccgacct ggctcgcgcg gcgcagatcc gcccgccggc
    11581 tccggtggcg ctcgaggatg aagtgcatcg cgcggccgac ctctgcaatc aggctcgggc
    11641 ggcggtgata tcgctcttca gcgaggcgcg catgggtcgc gtggtcttct ccgcggaatg
    11701 cgaagagctg gtgcatggca tcagcgattc ggtgatgcgc aacacgacgg ccatgctgtc
    11761 gctcgcccgc ctgaagagtc acgacgacta cacctacatg cattcggtcg cggtcgctgc
    11821 gctgatggcc gctctggcgc ggcagctcca gctcgagccg gacgagatcc ggatggctgc
    11881 caaggccggc ctgctgcacg acatgggcaa ggcggtcatg ccgagggaca ttctcaacaa
    11941 gccgggcaag ctgaccgacg aggagttcgt ggtcattcgc acccaccccg agcgcggcca
    12001 tgccttgctg atggctggcg aggtggccga cgaggcggtg ctggacgtgt gcctgcacca
    12061 ccacgagaag accgatggat cgggctatcc gcaccgcctg ccgggcgacc ggatctcgat
    12121 gttcgccaag atgggtgcgg tgtgcgatgt ctatgacgcc atcacctcca accgacctta
    12181 caagcagggc tgggatccgg ccgaatcgat cgcgcggatg gccggctggg cgaaggggca
    12241 cttcgacgcg gcgatcttca aggccttcgt caccagcctc ggcatctacc ccacgggttc
    12301 gctggtgcgg ctgaagtcgg gtctgctcgc ggtcgtcacg cgacagaatg acgccgcgct
    12361 gactgcgccg gaggtcaagg tgttcttctc gaccaagcag ggcctgcatg tgccgccccg
    12421 cttgctggac ctgtcgtccc ccggatgcac cgaccgcatc atcgttcgcg aatcgaacga
    12481 gggatggaaa ttcggtcatc tcgacgagct gtgggccggc gaggaagcgt tgcgcaagat
    12541 cggtcgcaag cgctgagccg atccgcgcgc cgcgtcgggc gcgcccctac actgaccgtt
    12601 tccggacagt cagggtggcc gatgaacgca gtgggttgga ggcgcaggac gatcggcgcg
    12661 gtggccgggc tgctgagcac ctgcgttctg attgcgggcg cctcggcgca gacgctgcga
    12721 tgggcctctc agggcgaccc ccagacgatg gatccgcact cccagaacga gttgctgacc
    12781 aacaacctca atgggcaggt ctacgagtcg ctggtgaacc gcgaccggcg gcttgacctg
    12841 gtgccggtgc tggccacttc atggcagcag aacggtccgt tgcgctggcg cttcaagttg
    12901 cggccgaacg tgaagttcca cgacggcacg ccgttcaccg cggacgacgt ggtgttctcg
    12961 gtgcgaaggg cgcgcgacag cgcctccgac atccgcgcct atgcgctggc gctcggcgaa
    13021 ccccaggccg tcgatgcgct gaccgtggac ttcgtcctga aggacgtcaa tccggtgttc
    13081 cttcaacacc tgtcgaccct gcagatcatg agtcgcgtgt ggtccgaaca tcacggcgcg
    13141 gtgaagccca tcgactaccg cagccgccag gaaggccacg cggccctgca cgccaacggc
    13201 accggcccct acaagctctc gctgcgccag cccgacgtga agaccgtcta ccagcgcaac
    13261 gctgcgtggt gggggacatt cgagggcaat gtgcaggaag tggtctacac gccgatcaag
    13321 agcgacgcga cacgcatcgc ggcgctgatc tctggcgagc tcgacttcgt gctcgacccg
    13381 gcgccccagg atctgccgcg cctgcgcagc acgcccgggg tcaaggtgac cgagggaccc
    13441 gagaaccgca tcgtcttcat cggtatggat caggcgcgcg accagctgct gtacggcagc
    13501 gtcaagggca cgaacccgtt caaggacctg cgtgtgcgca gggcgctcta ccaggcgatc
    13561 gacatcgaga cgattcgcag ccgcctgatg cggggccaat cgctgccgac cggcggcatc
    13621 acaccatctc cagtcggcgc ctacaacgac cccgaactgg aaaagcgcct gcccttcgac
    13681 ctgaacgctg cacggcagca gatgaccgat gccggctacc cgcagggttt cgaggtgacg
    13741 ctcgactgcc ccaacaaccg ctatatcaac gatgaggaaa tctgccagac gctggcggcg
    13801 atgtgggcca ggctcggcgt gaaggtgcgt gtcaatgcga tgccccgttc cacctatttc
    13861 ccgaagctgc agcggctcga cacctcgatg tacctgctcg gctggggggg cgccatcacc
    13921 gacgccgaga cgacgctgac cccgctgctg cgcagccggg gcgagggcgg catcggggcc
    13981 tggaacttcg ggcagttcag cgacgcccgc ctcgatcagc tcgcgcaggc gtccagccgg
    14041 gagggcgacc cgaagaagcg cgagcagctg atcaaggccg cgctgcagca gcagaaggat
    14101 caggtgcttt acctgccgct gcaccgtcag gtcgtgccct ggggggcgcg tcagaacgtc
    14161 gatctggtgc accgtgccga caactgggtc gagttctcgt ggatacgcat gcgatgaccg
    14221 atcgattcgt gcccgctgcg cagtcggacc cgcgtgcctt cctgcgggca ctctacgacg
    14281 ccgcggtgac ccgcgcgctg ccggccggcg tgatggcggc gagcctgccc gcaccgccgg
    14341 cgggtcgcac cgtggtggtc ggggccggca aggcgggggg ctcgatggcc gctgccgtcg
    14401 acgcgctgtg gcccgcgggc gcgccgctgt cgggcctggt ggtcacccgc tacgaccacg
    14461 tgccgccggc ttatcgagcc cgccccggcc gcatcgaggt cgtcgaggct gcgcacccgg
    14521 ttccagatgc cgccggcctg caggctgcgc gccggatcgt cgagctggtc cagggtctcg
    14581 gcgccgacga tctggtgctg tgtctgatct ccggaggggg gtcttcgctg ctgtcattgc
    14641 cggcgcaagg agtgacgctc gacgagaagc gggcgatcaa ccaggccctg ctgaagagcg
    14701 gcgctgcgat cgacgagatg aactgcgtgc gcaagcacct gtccgcgatc aagggcgggc
    14761 gactcgcggt gatgtgcgcc ccggctcgcg tggtcacact gctcgtcagc gacgtgccgg
    14821 gcgatgcgcc ggacgtcatc gccagcggcc cgacggtggc cgatcccacc acctgcgccg
    14881 acgccctggc gatcctccag cgctacggga tcgacctgcc cgaagccgcc cggcgcgggc
    14941 tggaaagcgg tgcgttcgag acaccgaagc ccggccatcc ggcgctggcc ggacaccagg
    15001 tgcacctgat cgcgacgcct cagcaatcgc tggaatccgc cgccgcggtg gcacgcgccg
    15061 ccggtgtcga cgcgcacatc ctgagcgacg agatcgaagg ggaatcgcgc gaggtcggca
    15121 aggtgcatgc ggcgctcgcg cgcgcggtgg cacgccgcgg cgcgccgttc gcccggccct
    15181 gcgtgatcct gagtggcggc gagaccacgg tgaccgtgcg gtcgggcggc ggtcgtggcg
    15241 gccgtgcgac cgagttcctg ctgggctgcg cgatcgcgct gcagggcgag cccggcgtgc
    15301 atgtgctggc agccgacacc gatggcatcg atggcatcga ggacaacgcc ggtgcgatcg
    15361 tcacgcccga cacgctgatc cgcgcccagg cgctcggaat gaaggcgcag gacttcctcg
    15421 accgcaacga tgcctacaac ttcttcgccc cgctgggcga cctcgtcgtg ccgggaccca
    15481 cctacacgaa tgtcaacgat ttccgggcac tgctgatcct gtagcgagct gcctggcctg
    15541 gcgggacaag ctgcggatcc cccacgtcgc cacagccgcc agaccgagca gcatcagcat
    15601 gccgagcgcc cacacggtcc agacgaccgg ggtcgcggcg cctccgatcc agcccagcac
    15661 ggtctgcatc agatcgacgc cgaacttcag catctcgcgc cagcccggaa tccaggcgtc
    15721 gatgaatgcc gcgtacggca ggcgctcggg cagttgatcg atcagcggct tcaggtcggc
    15781 gatgcgggac gcatcgagcg acagcagcgt gtgcagcccc caggcgccca gtgaccacag
    15841 caggcaactc agggcggtga tgatccacag ggcgatcagc atggaggtgg cccgaaggtg
    15901 gattggttgc gcatgtgttg tcgaccccgg tgtgtgagca ggcaggatgg attcgcgcgg
    15961 ccgccccgag ttcacccgac ctgctgcgct gaaaacaccc acgcggacga agctccgggc
    16021 gcctcgtgta aggtcatccg atgaacacag ccgttgaatc gactcgccgc cctgcctcgt
    16081 tgtggcctct ggcctggcgc cagctggcgc gggacttccg ggcgggcgag ttgcggctgc
    16141 tggtcctcgc ggtcacgctc gccgtggcgg cgctgacggc ggtcggcttc ttcgccgacc
    16201 ggatcaacgg cggcctcgca cgcgatgcga cgcaactgat gggcggagac gccatcgtcg
    16261 cgagcgacca gcccacgccg caggcgatcg tggacaaggc tcgctcgctg ggtctggcca
    16321 cggcgaccac cacggcgttt ccgagcatgg gccgcgccag cgacgagcgc ggtggagctt
    16381 cccgtctcgt cagcgtcaag gccgtgagcg acggctatcc cctgcgtggc cgattgcggc
    16441 tgagcgacgg tgccggcacg cccgaggtgt cggtggcgca gggcccccgg cccggcacgg
    16501 tctgggtcga atcggcgctg ctcgacgcgc tgcagctccg ggtaggcgac tccctgctgc
    16561 tcggcgatgc gacgctgcgc atcgctcagg tcatcgtgat cgagcccgac cggggcgccg
    16621 ggttcatgag cttttcaccg cgcgtgatgc tcaaccaggc cgatctgccg gccaccggac
    16681 tgatccagcc tgccagccgg gtgacctatc ggttgatcgt cgcggccccg gacagcaagg
    16741 agcgcagtga tccccgcgtg cgcgagttca ctcgctggac cgagtcgcag ctgaagtcgc
    16801 aggcgctgcg cggcgtgcga gtcgagtcgc tcgagggggg gcggcccgag atgcgccaga
    16861 cgctggaccg cgcggagaag ttcctcaacc tggtcgcgct gctgtcggcg ctgctggccg
    16921 cggtcgccgt gggcatcgca tcgcgggatt tcgccagccg gcggctcgac gactgcgcga
    16981 tgctgcgggt gctcggccag ccgcagcgca cgattgcgct tcagtacctg atcgagttca
    17041 cgctcgtcgg cctgctggcc agtgtcgcgg gtgtcgcgct ggggttcgtg gtgcatcacg
    17101 gcttcgtctg gctgctggcc ggactcgtca gcacgtcgaa cctgccgacc gcctcgtggt
    17161 ggcccgcgct tttcggtctc ggggtcggtt tcacgctgtt gatgggtttc gggatgcccc
    17221 cggtgcttca gctggcccgg gtgcctccgc tgcgggtgat ccgtcgcgac gtgggtgcgc
    17281 tgaagccggc gtcgatcgcg gtgctgctgg ccggtgcgct gggcttcgcg gcgttgctgc
    17341 tcgcggtgtc ctccgacgtg aagctcgggc tgatcgcggt gggcggcttt gctgctgcca
    17401 ttgcgatgtt cgcgttgctc agctgggccg cggtgctgct gctgcgccgg gccgtgcccg
    17461 aatcgagggc gccgcgctgg ctggtgctcg cgacgcgcca gatcgccgca cggccggctt
    17521 tcgcggtgct gcaggtgtcg gcgttgagcg tcgggctgct ggcgctggtg ctgctggtgc
    17581 tgctgcgcac cgacctcgtc agcagctggc gcgccgccac gccaccggat gcgccgaacc
    17641 gcttcgtgat caacgtgcag gccgggcagg gcgacgaatt caaggctcgg ctgcgccagg
    17701 ccggcatcgc gcagtacgac tggtacccga tgattcgcgg gcggctggtc gcgatcaacg
    17761 gcaagacgct gtcggccgac tcgatgaccg gagagcgcgc gcggcgcctc gtcgagcgcg
    17821 agttcaacct gagccattcg tcggtgctgc cgccgcacaa ccagctggtc gagggacgct
    17881 gggtgcccga ggaggacggc ggcctgagcg tcgaatcggg actggccgag accctcggcc
    17941 tgaagctcgg cgacacgctg cgcttcgaca tcgccgggca gccgacgcag gccagggtca
    18001 ccagcgtgcg caaggtcgac tggggttcga tgcgcgtcaa cttcttcgtg ctgttcccga
    18061 attcgacgct cgaggatgtg ccgatgacct acatcgcggc gttcaagacc ccggcggctg
    18121 gtggcgcgaa ggccgccgcc gaattcgaca acgcactgat ccgcctgttt cccaacatca
    18181 ccagcatcga cgtgtcggcg tcgatcgcgc aggtgcaggg cgtgctcgag caggtcatcg
    18241 gcgcggtcca gttcctcttc gtcttcacgc tggcagccgg gctggtcgtg ctgtttgccg
    18301 gggtcagcgc cacccgcgac gcgcgatcgc gggagttcgc ggtgatgcgc gcgctcggtg
    18361 cgagcggcag gctgctggcg caggtgcaac gggtcgaact tctgggcgtc ggggcgttgg
    18421 ccggcgtgct cgcgtctctg gccgcgcttg cggtgggctg ggtcctggcg cgtcaggtgt
    18481 tcgatttcaa ctggagcccg tcgccctggg tgccgttgat cggcggagtg accggtgcgc
    18541 tgctgtcgct ggccgcgggc tggtggggcc tgcgcgacgt gctgcgccgg ccggtggtcg
    18601 agacgctgcg ccaggccgcg caatgaagcg ttgacccgat cgcgggcggc ccaccgatga
    18661 aaaagcccgc cgctgctcag gcaggggcgg gcttggcgcg ccggggacgg atccccggca
    18721 ggcggcggtc agcttacttg cccgtacccg tggccacttc ttccagcacg ttcagcgaaa
    18781 cgtc
//
LOCUS       DAOORV010000069        18178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3744_length_18178_cov_9.444794, whole genome shotgun sequence.
ACCESSION   DAOORV010000069 DAOORV010000000
VERSION     DAOORV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18178
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3744_length_18178_cov_9.444794"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKV56_10540"
     CDS             <1..739
                     /locus_tag="PKV56_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX68293.1"
                     /translation="VPFEFSMHNFLLRYYLAEHGLNPDTDVQIRVVPPPEMVANLRAG
                     NIDGFLGPDPFNQRAVYDEVGFIHILSKEIWDGHPCCAFGVSEDFIKQNPNTFAALYR
                     SVLTAANMARDPKNRELIAKVISPTAYLNQPETVVAQVLTGKFADGLGGIKTVPDRAD
                     FDPVPWQSMAVWMLTQMKRWGYIKGDVNYKQIAEQVFLLTDAKKQMKLLDQKVPEGAY
                     RKYSIMGREFDPAKADAYAKSFAISRT"
     gene            809..1630
                     /gene="ntrB"
                     /locus_tag="PKV56_10545"
     CDS             809..1630
                     /gene="ntrB"
                     /locus_tag="PKV56_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HOX68294.1"
                     /translation="MVLLVWHLATLPAKSAPGPAVTLTPEQIEYQKLLGKDPAAAEGD
                     KKSSGFPTLAQMGATVVKNLADPLYDNGPNDKGIGLQLGYSLARVALGFGIAALVAIP
                     LGFLIGMSPMLYRALDPFIQVLKPISPLAWMPLALYTIKDSSISGIFVIFICSVWPML
                     INTAFGVAGVRREWLNVAKTLEVSRLRLAFEVILPAAAPTILTGMRISMGIAWLVIVA
                     AEMLVGGTGIGYFVWNEWNNLSLTNVIFAILVIGVVGMLLDLMFAKAQKAVSYVE"
     gene            1620..2498
                     /locus_tag="PKV56_10550"
     CDS             1620..2498
                     /locus_tag="PKV56_10550"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68295.1"
                     /translation="MSNETGFNTPSEAPFLRIEGLSKSYPSPDGKVSEPVFDSVNFGI
                     QKGEFVCIIGHSGCGKTTILNVLAGLDQATDGHVFMDGREVAGPSLERGVVFQGHALM
                     PWLSVRRNIAFAVRSKWPQWSASQVKVQVEKYVALVGLTPAIDKNPSALSGGMKQRVG
                     IARAFAIEPKMLLLDEPFGALDALTRGTIQDELLRICAETHQTVFMITHDVDEAILLA
                     DKILLMSNGPQARVAEIVRNTMPRDRQRATLHHDPQYYRIRNHLVDFLVARSKELSHG
                     RAPALPPVVEPGLAIA"
     gene            2533..2976
                     /gene="cynS"
                     /locus_tag="PKV56_10555"
     CDS             2533..2976
                     /gene="cynS"
                     /locus_tag="PKV56_10555"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HOX68296.1"
                     /translation="MNRNDVTEMIIEAKVKKGIKWDDVAKKVGLSKEWVTAGCLGQMT
                     FDAKQAKIVAKTFGLPAEAEKWLQVVPYKGSLPTSVPTDPLIYRFYELVNVYGTTFKE
                     LIHEEFGDGIMSAIDFKMDLTREPNAAGDRVSIVMSGKFLSYKTY"
     gene            3232..3702
                     /locus_tag="PKV56_10560"
     CDS             3232..3702
                     /locus_tag="PKV56_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOX68297.1"
                     /translation="MSETRPPLPPFTAETARQKVRMAEDAWNSRDPERVVGVYTEDTR
                     WRNRAEFPVGRAQVKEFLQRKWARELDYRLIKELWAFDGARIAVRFAYEFHDDSGQWF
                     RSYGNENWEFNLQGLMMRRYASINDLPIQADERLFHWPLGRRPDEHASLSDLGL"
     gene            complement(3725..5686)
                     /locus_tag="PKV56_10565"
     CDS             complement(3725..5686)
                     /locus_tag="PKV56_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOX68298.1"
                     /translation="MILPWFGGSSSVWSVCLVFFQVELLLGYAYVHWLHEKVAARRQP
                     LVHGALLLLSLALLPVGADPAWKGSTLSPGLSVLAVLATAVGAPYLLLSTTGPLMQTW
                     YARSFQSAGADARPYRLYALSNLASMLALLSYPVLVEPVLPVGTQAQLWSAAYVAFVA
                     TCLATAFFTRRRMAPVAPTAVIDAPAVPDVAPHPGWKECLIWIGLAATASMLLLSMTR
                     HLTQDVAPVPFLWIAPLSIYLLSFILCFDAPRYYVRPLFLAALLPAFLALDRVLDSGM
                     DVPILIALISASLFVFCMVCHGELVRRKPPVRHLTLFYLMLSIGGALGGFLVGLVAPA
                     IFNAYFELPIGLFLCAALVSVVLWRDLRTVWRVVLLIALAGYGWRLADISWEYVHGYR
                     KVVRNFYSQLRIDDSVDDVLGPKRKMVHGRINHGEQFMDPTLRREPTAYYCEKSGIGR
                     AIRSLQKETPLHIGVVGLGAGTLAAYGRAGDQMRIYEINEQVLELARSDFTFLSDSAS
                     RIEPVLGDGRLMLERDPPQHFDLLAMDAFSGDSIPAHLLTLEAMKTYFGQIKADGLLA
                     VHITNRYLDLEPVMAAVAQHYGKTALTYDLDPGDDDLYCRHTTWVLMMSSARAAALPQ
                     LLQDGVPLKPRPGFAPWTDSFSNLLKILK"
     gene            5925..6755
                     /locus_tag="PKV56_10570"
     CDS             5925..6755
                     /locus_tag="PKV56_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOX68299.1"
                     /translation="MTHPTRTLCSVALAAMSLFATQSALAAPNNPANDCAGLPSSAVL
                     EAALKAVVPAAAGGSASESNGGLDFPMWATVVNRYGVVCAVATSGTAADDAWLNSRVI
                     SAQKAYTANGFSRPTFALSTANLFTPTQNGNSLNGLQFSNPVDPRVVYRGNPTNYGTT
                     NDPMLGLKPGGINVFGGGVGLYSTGGKLGAVGVSGDTSCADHNIAWKLRHRLATAGFS
                     GVTIANRVPGGVRSAQVGSSNQPVAASGDDGIMYGNTGFQHADCGNSEKSVVLPAVTN
                     "
     gene            6767..7036
                     /locus_tag="PKV56_10575"
     CDS             6767..7036
                     /locus_tag="PKV56_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68300.1"
                     /translation="MAFQFNAAPPGDPGPLARLRLLGWGLCVTTMLLAGCGGDADDDT
                     AGGTGVPTATEIADAQSALDGTQPTSDSIDAKQAALDADADKDPE"
     gene            complement(7067..7426)
                     /locus_tag="PKV56_10580"
     CDS             complement(7067..7426)
                     /locus_tag="PKV56_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68301.1"
                     /translation="MDRLKVIRIAAGLTVLAVLVTTAVVVYRWRDAERVAALQRAETE
                     RTRKAFIQAAEAAERAAAATTPPEVLQARKDAALREFRAAETEMLLQQTRARLAREER
                     QAAAGNTPGPGASAPGP"
     gene            complement(7426..8565)
                     /locus_tag="PKV56_10585"
     CDS             complement(7426..8565)
                     /locus_tag="PKV56_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOX68302.1"
                     /translation="MTRVVALCAAVLGVLVGCSSAPTDTSPPADQPAPDARAPDAAPS
                     AASVQRGRARWVPARWSDLPGWEIDRTAELWPALLRGCEKPAAGWIGICAEARRSAPA
                     DDVSARAWLQRHLQPYRVESLDGSDQGLITGYFEPLIEARRTPQGRFRVPVHAPPNDL
                     SSRKPYWTRQQLDTLAPAQASVRGRELAYLTDPLDLLILQIQGSGRLWLTEADGSRRQ
                     LRVAFAGHNDQPYKSVGRWLIEQGELRADQASWPGIKAWAQLNPQRINELMWSNPRVV
                     FFREEPLPDPTVGPRGAQAVPLTPGRSVAIDPTSIPYGTVLWLDTTEPLSNTPLRRVV
                     MAQDTGSAITGAVRVDYFWGWGDTAEQQAGRMKQPLRVWVLAPRN"
     gene            complement(8701..10746)
                     /locus_tag="PKV56_10590"
     CDS             complement(8701..10746)
                     /locus_tag="PKV56_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HOX68303.1"
                     /translation="MSASSSVWDLTALLNAAAHASDPKAPLPDCNLWVIRALEWLRHP
                     PMSGAGESAADEAAAVATPATPPPLLRLKHLLNVLDRHPEHRAQVAGVLGRFWGAMDS
                     AALFADFGFSSRANFFGEIGQRLKLKLLPLTPASSDPSELFALLFPGDDDGAWMESLD
                     QPTLMRLLGLVPGNPADERAWLTPLLDAIMYLASAVRAAGFSGALRRRMSPELLVEAP
                     FRQLAVVAERIRELANTPTRDDAALLREAQYLRALLDSCRRCADSVHGHLEEHGVSIN
                     VVFDVDQLKQRTHRIEQLLNCVISPEPAREVTRLFAELVRTAADRRSIRALFARHYSL
                     LARKVAERSAETGDHYITRDGAAYRHMLRAAMGGGAVIAGTTFVKFLVLALGLSAFWG
                     GFWAGMNYAVSFVIVHLLHWTVATKQPAMTAPALAEKLSDVADDPAVERFVDEVANLI
                     RSQAAGIFGNLAAVIPLVLLVQWASQAVFGAPLVQGHAAEHVLDSITLLGPTALFAAF
                     TGVLLFASSLIAGWVENWFVWHRLDSAIAWNPRIVDRLGVARAQRWAAYWRANISGFA
                     SNISLGLMLGIVPAVALFFGLPLEVRHVTLSTGQLAAAAAAQGLSVLHHGPFWWCVAG
                     IVVTGLLNLTVSFFLAFRVAMRSRGIRLADRSRIRQAIARRLRSHPGSFVLPPQAVD"
     gene            complement(10787..11161)
                     /gene="apaG"
                     /locus_tag="PKV56_10595"
     CDS             complement(10787..11161)
                     /gene="apaG"
                     /locus_tag="PKV56_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOX68304.1"
                     /translation="MARPEFTCSVTVRHLAEQSDPKEHQYAFAYTVTIHNTGDVTGQL
                     IARHWIITDAENHVEEVRGLAVVGHQPLLKPGEHFEYTSWTRIATPLGTMRGTFICMT
                     EEAEPFDAAVPEFGLTLASALH"
     gene            11278..12783
                     /gene="trpE"
                     /locus_tag="PKV56_10600"
     CDS             11278..12783
                     /gene="trpE"
                     /locus_tag="PKV56_10600"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOX68305.1"
                     /translation="MITDTEFQRLADEGYNRIPLIAQAFADLETPLSLYLKLACTDDS
                     GQPSDTHRRHSFLLESVVGGERFGRYSFIGLPARTMLRATGFLTEVLTDGAVIERHEG
                     NPLDFIAAYQQRFKVALQPGLPRFCGGLAGYFGYDAVRAIEPKLAHTELPGGLGTPDI
                     LLLQCEELAVIDNLSGRLSLIVYADPAQADALPRAKRRLSALADKLKNSVQAPAVERR
                     TPNEVLRDFAKDDYIAAVLKAKEYIAAGDMMQVQVGQRLRKPYAGNPLSLYRALRSLN
                     PSPYMYFYDMGDFQVVGASPEILVRREHTPAGDKVTIRPLAGTRPRGSTPEHDLALEA
                     ELSADPKERAEHVMLIDLARNDIGRIAKTGSVKVTEAFTVERYSHVMHIVSNVEGLLK
                     DGITNLDVLKATFPAGTLTGAPKVRAMEIIDELEPVKRGIYGGACGYLSFAGDMDVAI
                     AIRTGIVKDGMLYVQAAAGVVADSVPELEWRETEAKARALIRAAELVEEGF"
     gene            complement(13135..14289)
                     /gene="rodA"
                     /locus_tag="PKV56_10605"
     CDS             complement(13135..14289)
                     /gene="rodA"
                     /locus_tag="PKV56_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOX68306.1"
                     /translation="MRPAFEKPSLLQRMIPLFRGFDGPLLLAVLILAALGLTTMYSAG
                     FDHGTRFVDHARNMLLAAGILFVVAQIPPQRLMALAVPLYALGVALLVATALIGITKK
                     GATRWLDLGVVIQPSEILKIAMPLMLAWWFQRREGQVRALDFIVALLILAVPVGLIVK
                     QPDLGTAILVLAAGLFVIFFAGLPWKLIAPVVAVAVIAIAVLVLSGDAICQPDVKWPM
                     LRDYQKNRVCTLLDPTTDPLGKGFHIIQGMIAIGSGGITGKGFMKGTQTHLEFIPERT
                     TDFIFAAYSEEFGLIGCVVLLLGFLFLIVRGLMIASEAPNVFTRLLAGSITLSFFTYA
                     FVNMGMVSGILPVVGVPLPFISYGGTAMVILGLGLGILMSIAKSRRLVQG"
     gene            complement(14286..16382)
                     /gene="mrdA"
                     /locus_tag="PKV56_10610"
     CDS             complement(14286..16382)
                     /gene="mrdA"
                     /locus_tag="PKV56_10610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOX68307.1"
                     /translation="MTEIRNVEQELSRFHARLVAATAFVLLCFGLLGYRLVHLQIVKH
                     DELSVQAENNRISVVPIVPNRGLIVDRNGVVLANNYSAYTLEIMPARVQDLESTISQL
                     SEVIDIQTRDRKRFKRLMDESKNFESLPIRTKLTDEEVARFTAQRYRFPGVDVKARLF
                     RNYPLGDVGSHLLGYIGRINQAEKEVIDELEDAANYRGTEYIGKLGIEQSYESDLHGT
                     TGFEEVETSASGRAVRRLRSNPATPGNTVVLSIDIRLQALVETLFGDRRGALVAIDPR
                     NGEVLAFVSKPTFDPNLFVDGIDSESWKELNESPDKPLLNRALRGTYPPGSTYKPFMA
                     MAALNSNKRSPSVLINDTGSFTFGGHRYGSPENERGGIMDMRRAIVESSNVYFYTLAN
                     EMGVDLIHDQLSPFGFGRKTGIDVEGEVTGLLPSTEWKRRAYKKPEQKKWYAGETISL
                     GIGQGYNSFTMLQMATAMATLSSAGKRYEPRLVRQIDDVLTGAQRKISAQALEPVELK
                     PEQVDVIREAMNGVTIAGTSARVFAGAGYQSGGKTGTAQAVGLKQNEKYSAHRVDEHL
                     RDHALYIAFAPLADPQIAVAVIVENAGWGAGAAAPIARRLFDFMLLGRVPSEEDIAAT
                     KSGTSAAPIGQPRNVQDLVLPGSGSTPSAAAAMIGVASAPATSLAAAPMAVSGGASLV
                     SANAAAETPDNRALRR"
     gene            complement(16500..17021)
                     /gene="mreD"
                     /locus_tag="PKV56_10615"
     CDS             complement(16500..17021)
                     /gene="mreD"
                     /locus_tag="PKV56_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOX68308.1"
                     /translation="MIMPRSSDQLLLPVNPLFLWFTLLTALALNLVPLGRHPAAPDLL
                     ALALVFWNVHQVRRVSVGVAFMFGLVMDVHDGAILGQHALAYTLLGFVATTIHRRLLW
                     FTLPSQALQILPLFVAAHLVSFAVRMIAGGMLPGWQALLAPAFEALLWPLATWLLLAP
                     QRRAPDRDQNRPL"
     gene            complement(17018..17959)
                     /gene="mreC"
                     /locus_tag="PKV56_10620"
     CDS             complement(17018..17959)
                     /gene="mreC"
                     /locus_tag="PKV56_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOX68309.1"
                     /translation="MPLGTLDRTPPPFFRQGPSALTKLALCSALAVFLMVADTRFTLT
                     QPLRALLATALHPIQRTLLAPVDWSEQAADYAGGLQHAIAAESAARRELAAQAVHVAR
                     ANQLQAENERLRTLLELRAGLTVRSRAAQVLYESTDPYSRKVVIDQGLAQGIVAGSPV
                     VSDAGVIGQVTRAYPLSSEVTLLTDKDAAVPVLNARTMARSAAFGGATGDSLELRFMA
                     GNADVQVGDLLQTSGVDGVYPPGLPVARVVTVDRKVDSGFAKIMLVPAALPDGVRHVL
                     VLEPIGLQLPPRPEPNVDEPTAAASSASAPARKGARR"
     gene            complement(18029..>18178)
                     /locus_tag="PKV56_10625"
     CDS             complement(18029..>18178)
                     /locus_tag="PKV56_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOX68310.1"
                     /translation="ALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGMALERMERLGS
                     IFTSE"
BASE COUNT         3342 a         6052 c         6023 g         2761 t
ORIGIN      
        1 ggtgccgttc gagttctcga tgcacaactt cctgctgcgc tactacctgg cagagcacgg
       61 cctgaacccg gacaccgacg tgcagatccg cgtcgtgccg ccacccgaga tggtggccaa
      121 cctgcgcgcc ggcaacatcg acggcttcct tggcccggac ccgttcaacc agcgcgcggt
      181 gtacgacgag gtgggcttca tccacatcct ctcgaaggaa atctgggacg gccacccctg
      241 ctgcgccttc ggggtgagcg aagacttcat caagcagaac ccgaacacct tcgccgcgct
      301 ctaccgctcg gtgctgaccg ctgccaacat ggctcgtgac ccgaagaatc gcgagctgat
      361 cgccaaggtc atctcgccca ccgcctacct gaaccagccc gagacggtgg tggcccaggt
      421 cctgaccggc aagttcgccg atggcctcgg cggcatcaag accgttcccg accgcgcaga
      481 cttcgacccg gtgccgtggc agagcatggc ggtgtggatg ctgacccaga tgaagcgctg
      541 gggctacatc aagggcgatg tcaactacaa gcagatcgcc gagcaggtgt tcctgctgac
      601 cgacgccaag aagcagatga agctgctcga tcagaaggtt ccggagggcg cgtacaggaa
      661 gtactcgatc atgggccgcg agttcgaccc ggccaaggcc gacgcgtacg cgaagagctt
      721 cgcgatcagc cgcacctgag gcggcacggt gaagactccc cgcaaggagg cgctgcagtc
      781 cggcgccttg tcggtgttca tcctggtcat ggtgctgctg gtctggcacc tggccacgct
      841 gcccgccaag tcggcgcccg ggccggccgt cacgctgacc cccgaacaga tcgagtacca
      901 gaagctgctc ggcaaggatc cggccgccgc cgaaggcgac aagaagagca gcggcttccc
      961 gacgctcgcg cagatgggcg cgacggtggt caagaacctc gccgatccgc tgtacgacaa
     1021 cggccccaac gacaagggca tcgggctcca gctcggctat tcgctggccc gcgtcgcgct
     1081 cggcttcggc atcgccgcgc tggtggcgat cccgctggga ttcctgatcg gcatgagccc
     1141 gatgctctac cgtgcgctcg atccgttcat ccaggtcctg aaaccgatct cgccgctggc
     1201 ctggatgcca ctggcgctct acacgatcaa ggactcgtcg atctccggca tcttcgtgat
     1261 cttcatctgc tcggtgtggc cgatgctgat caacaccgcg ttcggtgtcg ccggcgtccg
     1321 gcgtgaatgg ctcaacgtcg cgaagacgct cgaggtatcg cggctgcgcc tcgcgttcga
     1381 ggtgatcctg cctgccgcag cgccgacgat cctgaccggc atgcgcatca gcatgggcat
     1441 cgcgtggctg gtgatcgtcg cggccgagat gctggtcggc ggcaccggca tcggctattt
     1501 cgtctggaac gagtggaaca acctgtcgct gaccaacgtg atcttcgcga tcctggtgat
     1561 cggcgtcgtc gggatgctgc tcgacctgat gttcgcgaaa gcgcagaagg cggtgtcgta
     1621 tgtcgaatga aaccggcttc aacacgccgt ccgaagcgcc cttcctgcgc atcgagggtc
     1681 tgtcgaaaag ctatccgtca ccggacggga aggtcagcga gccggtcttc gacagcgtca
     1741 acttcggcat ccagaagggt gagttcgtct gcatcatcgg ccactcgggc tgcggaaaga
     1801 cgacgatcct gaacgtgctg gcgggtctcg accaggccac cgacggccac gtgttcatgg
     1861 atggccgtga agtcgcggga ccgagcctgg agcgcggtgt cgtgttccag gggcacgcct
     1921 tgatgccgtg gctgtcggtg cgcaggaaca tcgcctttgc cgtgcgcagc aagtggcccc
     1981 agtggagcgc ttcccaggtg aaggtgcagg tcgagaagta cgttgcgctg gtcggcctga
     2041 cgccggccat cgacaagaac ccgtcggcgc tgtcgggcgg catgaaacag cgcgtgggta
     2101 tcgcgcgagc gtttgcgatc gagccgaaga tgctgctgct cgacgaaccg ttcggcgcac
     2161 tcgatgcact gacccgcggc acgatccagg acgagctgct gcgcatctgt gccgagacgc
     2221 accagacggt gttcatgatc acgcacgacg tcgacgaggc catcctgctg gccgacaaga
     2281 tcctgctgat gagcaacggg ccgcaggcgc gggtcgccga gatcgttcgc aacacgatgc
     2341 cgcgcgaccg gcagcgcgcc acgctgcacc acgatccgca gtactaccgc atccgcaacc
     2401 acctggtcga cttcctcgtc gcacgctcga aggagctgtc gcacggccgg gcccccgcac
     2461 tgcccccggt cgtcgagccc ggactcgcga tcgcctgaaa caactttcca acacgcaaag
     2521 caaggagcaa ccatgaaccg caatgacgtc accgaaatga tcatcgaggc caaggtcaag
     2581 aagggcatca aatgggatga cgtggccaag aaggtcggcc tcagcaagga atgggtcacc
     2641 gcgggatgcc tggggcagat gaccttcgac gccaagcagg ccaagatcgt cgcgaagacc
     2701 ttcgggctgc ccgccgaggc cgagaaatgg ctgcaggtgg tgccatacaa gggctctctg
     2761 ccgacctcgg taccgaccga cccgctgatt tatcgttttt acgagctcgt gaacgtctac
     2821 ggcacgacgt tcaaggaact gatccacgag gaattcggcg acggcatcat gagcgcgatc
     2881 gacttcaaga tggacctgac gcgcgagccg aacgccgcgg gcgaccgggt cagcatcgtg
     2941 atgtcgggca agttcctgtc gtacaagacc tactgatccg gaaccgcatt tgattgcagg
     3001 cttgggagcg gtactgccga gggcgccgtg gttgcacacg aaggcattca cgaccgcttc
     3061 ggtggtggca ggagcgctgc gccgagtcgc tgtgtggagt gaagtaaggg ggagagtcgg
     3121 gcgcagcccg gctcggagga catgagcgca acacagcaag tcggcgcagc gctcccgtac
     3181 tgaactcgat tcaattgaca ggtctgaacg caactcggta ggaagcacat catgagcgaa
     3241 acccgccccc cactgccgcc cttcaccgca gagaccgcac gccagaaggt gcgcatggcc
     3301 gaggacgcct ggaactcgcg cgaccccgaa cgcgtggtcg gcgtctacac cgaggacacg
     3361 cgctggcgca accgtgcgga gtttccggtc ggccgcgcgc aggtgaagga gttcctgcag
     3421 cgcaagtggg cgcgtgagct cgactaccgg ctgatcaagg agctgtgggc gttcgacggc
     3481 gcgcgcatcg cggtgcgctt cgcctacgag ttccacgacg actcgggaca gtggttccgc
     3541 agctacggca acgagaactg ggagttcaac ctgcagggat tgatgatgcg gcgctacgcc
     3601 agcatcaacg acctgccgat ccaggccgac gaacggctct tccactggcc gctcggcagg
     3661 cgccccgatg aacacgcgag cctgagcgac ctcgggctgt gacggcttgc cccggtgcga
     3721 gcggttactt gaggatcttg agcaggttag agaaactgtc ggtccacggc gcgaagccgg
     3781 ggcgcggctt cagcggaaca ccgtcctgca gcaactgcgg caatgcggcg gcgcgcgcgg
     3841 agctcatcat cagcacccag gtcgtgtgac ggcagtacag atcatcgtcc ccgggatcca
     3901 ggtcataggt cagcgcggtc ttgccgtagt gctgcgcgac ggccgccatc accggctcca
     3961 ggtccaggta gcggttggtg atgtgcaccg cgagcaggcc gtcggccttg atctgcccga
     4021 agtaggtctt catcgcttcc agcgtcagca ggtgcgccgg gatcgaatcg cctgaaaacg
     4081 cgtccatcgc gagcaggtcg aagtgctgcg gcggatcgcg ctcgagcatc aggcggccat
     4141 ccccgagcac cggctcgatg cgcgacgcgc tgtcggacag gaaggtgaag tcgctgcgcg
     4201 ccagttcgag cacctgctcg ttgatctcgt agatgcgcat ctggtcgcct gcgcgcccgt
     4261 aggcggccag cgtgccggcg ccgagaccca ccacgccgat atgcaagggc gtctccttct
     4321 ggaggctgcg gatcgcgcgg ccgatgcccg atttctcgca gtagtaggcg gtcggctccc
     4381 tgcgcagcgt cggatccatg aactgctcgc cgtggttgat ccggccgtgc accatcttgc
     4441 gcttcggccc gagcacgtca tcgacgctgt cgtcgatgcg cagctggctg tagaaattgc
     4501 gcacgacctt gcggtagccg tgcacgtatt cccaggagat gtccgccaat cgccagccgt
     4561 accccgccag ggcgatcagg agcaccacgc gccacacggt gcgcagatcg cgccacagca
     4621 ccacgctcac cagcgccgcg cagaggaaca ggccgatcgg cagttcgaag taggcattga
     4681 agatcgctgg ggccaccagc ccgaccagaa acccgccgag cgccccgccg atcgacagca
     4741 tcagatagaa cagcgtgaga tggcgcaccg gaggcttgcg cctgaccagt tctccgtggc
     4801 acaccatgca gaagacaaac agcgacgcac tgatcagcgc gatcaggatc ggcacgtcca
     4861 tgccgctgtc gagcacccgg tcaagcgcca ggaacgccgg cagcaacgcg gccaggaaca
     4921 acggacgcac gtagtagcgc ggcgcgtcga agcacaggat gaaactgagc aggtagatgg
     4981 acaggggcgc aatccacagg aacggcaccg gtgccacgtc ctgcgtcaga tgacgcgtca
     5041 tcgacaacag cagcatcgat gcggtggcgg ccaggccgat ccagatcagg cactccttcc
     5101 agccgggatg cggcgcaacg tccggcacgg caggcgcatc gatcacggct gtcggcgcaa
     5161 caggcgccat gcggcgccgc gtgaagaagg ccgtcgccag acaggtcgcg acgaaagcca
     5221 cgtaggccgc cgaccacagt tgcgcctgcg tgcccaccgg cagcaccggc tcgaccagca
     5281 ccgggtacga caagagggcc agcatcgacg ccaggttcga cagcgcatac agccggtagg
     5341 gtctggcgtc ggctccggca ctctggaacg agcgcgcgta ccaggtctgc atcagcgggc
     5401 cggtcgtcga cagcagcaga tagggagcgc ccaccgcggt ggccagcacg gccagcacgc
     5461 tcaggcccgg ggacagcgtg gaacctttcc atgcggggtc ggcgccgacc ggcagcagtg
     5521 ccaggctcag cagcagcaag gccccatgca cgagcggctg cctgcgggcc gcgaccttct
     5581 cgtgcagcca atgcacatag gcgtatccca gcaggagctc gacctggaag aagaccagac
     5641 acacgctcca caccgaagac gacccgccga accacggcag gatcatcttc gccacgatgg
     5701 gctggatctg gaagagaagg aaagctgaga ggaaaaccgt tactgcgaaa aggcccatcg
     5761 gtcgaggata acaacgaggc ccgccagaac aggtcgcgcc gcgctgtgaa cgcggcccga
     5821 atcggttctt catttccagg gtgtctaggg tgtccggggg tgtacttgcc ccgcgactcg
     5881 tggcttgatg tgaacccgac tgactcaaca agggagaccc cgaagtgacc caccccaccc
     5941 gtaccctgtg ctctgtggcc ctggccgcga tgagcctttt cgcgacccag agtgccctgg
     6001 ctgcaccgaa caatcccgcc aacgactgcg ccggcctgcc cagcagcgcg gtccttgagg
     6061 ccgccctgaa ggcggtggtg ccggcagcag ccggcggctc cgcttcggag tcgaatggcg
     6121 gtctcgactt cccgatgtgg gccacggtcg tcaaccggta cggtgtcgtc tgtgcggtcg
     6181 cgacctcggg cacggcagcc gatgacgcat ggctgaacag tcgggtgatc tcggcgcaga
     6241 aggcctacac cgccaacgga ttcagccgcc cgactttcgc actatcgacc gccaacctgt
     6301 tcaccccgac gcagaacggc aactcgctga acggcctgca gttcagcaac ccggtcgacc
     6361 cgcgcgtggt ctaccgcggc aacccgacca attacggcac caccaacgac ccgatgctcg
     6421 gattgaagcc gggcggcatc aatgtgttcg gcggcggcgt cggcctctac agcaccggcg
     6481 gcaagctcgg ggcggtgggc gtcagcggcg acacctcgtg cgctgatcac aacatcgcct
     6541 ggaagctgcg gcaccgactc gccaccgccg gcttctccgg cgtgaccatc gccaatcgcg
     6601 tgcccggcgg cgtgcgcagc gcgcaagtgg gttcgtccaa ccagcctgtc gctgcaagcg
     6661 gcgacgacgg catcatgtac ggcaacacag ggttccagca tgccgattgc ggcaacagcg
     6721 agaagagcgt cgtcctgcct gccgtgacga attgaggagg aacgcgatgg ccttccaatt
     6781 caacgccgca ccgcccggcg accccggacc gctggcccgg ctccggctgc tcggctgggg
     6841 actctgtgtg acgacgatgc tgctggcagg gtgtggcggc gatgccgacg atgacaccgc
     6901 cggcggtacg ggcgtgccca cggccaccga gatcgcggac gcgcagtccg cgctcgacgg
     6961 gacccagccc acgagcgaca gcatcgacgc caagcaagcg gccctcgacg ccgacgccga
     7021 caaggacccc gagtaggcga acgcgcgtcg cctcgtgagc ccgacctcag ggtccgggag
     7081 cagatgctcc cggacccggg gtgttgcctg cggcggcctg ccgctcctca cgcgccagcc
     7141 tggcccgtgt ctgctgcagg agcatctcgg tttcggcagc tcgaaactcg cgcagtgccg
     7201 catccttgcg tgcctgcaac acctcgggtg gcgtcgtcgc cgctgcggca cgctcggccg
     7261 cttcggccgc ctgaatgaat gcctttcggg tgcgctcggt ctcggctcgc tgcaacgccg
     7321 cgacccgttc ggcatcccgc caccggtaaa cgacgacggc agtggtgacc aggacggcga
     7381 gcaccgtcag gcccgcagca atccgaatga ccttgagccg gtccatcagt tgcgtggcgc
     7441 cagcacccag acccgcaagg gctgcttcat gcggccggcc tgctgctcgg cggtgtcgcc
     7501 ccatccccag aaatagtcca cccggaccgc accggtgatg gcactcccgg tgtcctgcgc
     7561 catcacgacg cggcgcaggg gcgtgttcga cagcggctcc gtggtgtcga gccagagcac
     7621 cgtcccgtag gggatgctgg tcgggtcgat cgcgacggag cggcccgggg tcagtggaac
     7681 cgcctgcgcg ccgcggggcc cgacggtcgg gtcaggcagc ggctcctcgc ggaagaagac
     7741 cacgcggggg ttggaccaca tcaactcgtt gattcgctgc ggattcagtt gcgcccaggc
     7801 cttgatgccg ggccacgacg cctgatcggc acgcaactcg ccctgctcga tcagccagcg
     7861 accgaccgac ttgtaaggct gatcgttgtg cccggcgaac gcgacacgca actgccggcg
     7921 gctgccgtcg gcttcggtca gccacagtcg acccgaaccc tggatctgca ggatcagcag
     7981 gtcgagcggg tcggtgaggt aggccagttc gcggccacgc acgctggcct gcgcgggcgc
     8041 cagcgtgtcg agttgctggc gggtccagta gggcttgcgg ctggacagat cgttcggcgg
     8101 tgcatgaacc ggcacgcgga accttccctg cggcgtgcgg cgcgcctcga tcagtggttc
     8161 gaagtagccg gtgatcaaac cctgatccga tccgtcgagc gactcgaccc ggtagggttg
     8221 cagatggcgc tgaagccagg cccgcgcgct gacgtcgtcc gccggggcac tgcgacgcgc
     8281 ttcggcgcag atgccgatcc agcccgcggc cggcttctcg cagccccgaa gcagcgcggg
     8341 ccacagctcc gcggtgcgat cgatctccca gcccggcagg tcactccagc gcgccggcac
     8401 ccagcgagcc cggccgcgtt gcacgctggc tgcgctgggt gcagcgtcag gagcccgcgc
     8461 gtccggcgcc ggttggtcgg ccggcgggga ggtgtcagtc ggcgcgctgg agcacccgac
     8521 cagcacgccc agaaccgctg cgcacaaagc gacgacgcgt gtcatgtgcc tgttcctgga
     8581 agcgctcgac gccacggtgc tcatgatgct gaggtctgag taaggatgct ttcaagcggg
     8641 aaccgcggcg ggcggggcgg gacggagggt cgacactgaa ccgcaaggcc accgcgcaat
     8701 tcaatcgacg gcctgggggg gcaacacgaa cgaacccggg tggctgcgca gccgccttgc
     8761 aatcgcctgc cggatgcgac tgcggtcggc gagccggatg ccgcgtgagc gcatcgcgac
     8821 cctgaacgcc aggaagaagc tcaccgtcag gttcagcagt ccggtgacga cgatgccggc
     8881 cacacaccac cagaaggggc cgtggtgcaa cacggacagg ccctgcgcgg cggctgcggc
     8941 ggcgagctgg ccggtcgaca gggtgacgtg ccgcacctcc agcggcaggc cgaagaacag
     9001 ggccaccgcc gggacgatgc cgagcatcag gcccagcgag atgttcgacg cgaaacccga
     9061 gatgttggcc cgccagtagg ccgcccagcg ctgcgcgcga gccacgccca ggcgatcgac
     9121 gatgcgcggg ttccaggcaa tcgcgctgtc aaggcggtgc cagacgaacc agttctcgac
     9181 ccagccggca atcagcgaac tggcaaacag cagcaccccg gtgaaggctg caaagagcgc
     9241 cgtcgggccg agcagcgtga tcgagtccag cacatgctcg gccgcgtgtc cctgaaccag
     9301 cggcgcgccg aagacagcct gcgacgccca ctgcaccagc agcacgagcg ggatcacggc
     9361 ggccaggttg ccgaagatgc cggccgcttg cgagcggatc aggttcgcaa cctcgtcgac
     9421 gaagcgttcg accgcggggt cgtcagcgac gtccgacagc ttctcggcca gcgcgggcgc
     9481 agtcatcgca ggctgcttgg tggccaccgt ccagtgcagc agatgcacga tgacgaagct
     9541 gaccgcgtag ttcatgccgg cccagaagcc gccccagaac gccgacaggc ccagcgcaag
     9601 cacgaggaac ttgacgaatg tcgtccccgc gatcaccgcg ccgccgccca tcgccgcgcg
     9661 cagcatgtga cggtaggcgg cgccatcccg ggtgatgtag tgatcgccgg tttcagcact
     9721 gcgctcggcg accttgcggg ccagcagcga gtagtgccgc gcgaacagcg cgcggatgct
     9781 gcggcggtcg gcagcggtgc gcaccagttc ggcgaacagt cgcgtcacct cgcgcgccgg
     9841 ctcggggctg atgacgcagt tgagcaactg ctcgatgcgg tgggttcgtt gcttcaactg
     9901 atcgacatcg aacacgacat tgatcgatac gccgtgttcc tccaggtgcc cgtgcacgct
     9961 gtcggcgcac cggcggcacg agtccagcag cgcacgcagg tactgtgctt cgcgcagcag
    10021 cgccgcgtcg tcccgggtcg gcgtgttcgc cagttcccgg atgcgttcgg cgacgaccgc
    10081 gagctggcga aacggcgcct cgaccagcag ttcggggctc atgcgcctgc gcaacgcgcc
    10141 ggaaaatccg gccgcgcgaa ccgcactggc cagatacatg atggcgtcga gcaacggtgt
    10201 cagccaggca cgctcatccg ccggattgcc gggcaccagg cccagcaagc gcatcagagt
    10261 gggctggtcc agcgactcca tccaggcccc gtcgtcatca cccggaaaca gcagcgcaaa
    10321 cagttcggac ggatcgctgc tggcaggcgt cagcggcagc agcttgagtt tcagccgctg
    10381 gccgatctca ccgaaaaaat tggcgcgaga cgaaaacccg aaatcggcga acagtgcggc
    10441 gctgtccatc gcgccccaga agcgtcccag cacaccggcc acctgggcgc ggtgctcggg
    10501 gtgacgatcg aggacattga gcaggtgctt gagcctgagc agcggcggtg gagtcgcggg
    10561 cgtcgcaact gcggcagctt catcggcagc ggattcaccg gcgccggaca tcggcgggtg
    10621 gcgcagccac tcgagcgcgc ggatgaccca gagattgcag tcgggcagcg gcgccttggg
    10681 atcggacgcg tgggcggctg cattgagcag tgccgtgaga tcccacaccg acgaggacgc
    10741 ggacatcccg gccactgcgg tcaacggctg ccgaccgacc gctgcatcag tgcagcgccg
    10801 aagcgagcgt cagcccgaac tcgggaaccg ccgcgtcgaa gggttcggcc tcctcggtca
    10861 tgcagatgaa ggtgccacgc atcgtgccca gaggcgtggc gatacgcgtc cagctcgtgt
    10921 actcgaaatg ctcgccgggc ttgagcagcg gctggtggcc gaccacggcg agaccccgga
    10981 cctcttcgac atggttctcg gcgtcggtga tgatccagtg acgggcaatc aactggcccg
    11041 tgacgtcccc ggtgttgtgg atggtcaccg tgtaggcaaa tgcgtactgg tgctccttgg
    11101 ggtcggattg ctcggcgaga tgccgcacgg tgacagagca ggtgaattca gggcgggcca
    11161 tcgagccatt ctaggagccc gcccgagtcg atgccgaaag ccctggcgca ggcaccgccc
    11221 tgataatgcg caccctggcc gccgggcgag gtcccgggcc gtggagggcc ccgcaacgtg
    11281 atcaccgaca ctgaattcca acgcctcgcc gacgagggct acaaccgcat tccgctgatc
    11341 gcgcaggcct ttgcggatct ggagacgccg ctatcgctgt acctgaagct cgcctgcacc
    11401 gacgacagcg gccagccgtc ggacacgcat cggcgccaca gtttcctgct cgaatccgtg
    11461 gtcggaggcg agcgcttcgg ccgctattcg ttcatcggcc tgcccgctcg cacgatgctg
    11521 cgagccaccg gattcctgac cgaggtgctg accgacggtg ccgtgatcga gcggcatgag
    11581 ggcaacccgc tcgacttcat cgccgcctac cagcagcgct tcaaggtcgc actgcaaccc
    11641 ggacttccgc gcttctgcgg cggcctggca ggctacttcg gctacgacgc ggtgcgcgcg
    11701 atcgaaccga agctggcgca caccgaattg cccggcggtc tcggaacgcc ggacatcctg
    11761 ctgctgcagt gcgaagagct ggccgtgatc gacaacctgt cggggcgact gtcgctgatc
    11821 gtgtacgccg acccggcgca agccgatgca ctgccgcgtg ccaagcgacg cctctcggcg
    11881 ctggccgaca agctgaagaa cagcgtgcag gcacctgcgg tcgagcggcg caccccgaac
    11941 gaggtgctgc gggacttcgc caaggacgac tacatcgccg ccgtgctgaa ggccaaggaa
    12001 tacatcgccg ccggcgacat gatgcaggtg caggtggggc agcgcctgcg caagccctac
    12061 gccgggaacc cgctcagtct gtaccgtgcg ctgcgatcgc tgaacccctc gccgtacatg
    12121 tatttctacg acatgggcga tttccaggtc gttggcgcgt cgccggaaat cctggtccgc
    12181 cgcgagcaca cgccagcggg cgacaaggtg acgatccgcc cactggccgg cacgcgcccg
    12241 cgcggcagca cacccgaaca cgatctggca ctggaagccg aactgagcgc cgacccgaag
    12301 gaacgcgccg agcacgtgat gctgatcgac ctggcacgca acgacatcgg ccgcatcgcg
    12361 aagaccggca gcgtgaaagt caccgaggcg ttcacggtcg agcgctactc gcacgtgatg
    12421 cacatcgtca gcaacgtcga aggcctgctg aaggacggca tcaccaacct cgacgtgctg
    12481 aaggcgacct tcccggccgg cacgctgacc ggtgcgccga aggtgcgcgc gatggagatc
    12541 atcgacgagc tggagcctgt caagcgcggc atctatggcg gcgcctgcgg ctacctgagc
    12601 tttgccgggg acatggacgt ggcgatcgcg atccgcaccg gcatcgtcaa ggacggcatg
    12661 ctctatgtgc aggcggcagc cggcgtggtg gccgactcgg tgcccgagct cgaatggcgc
    12721 gagaccgaag ccaaggcccg cgcgctgatc cgcgcggcag agctggtcga agaggggttc
    12781 tgatacacgg tcgcgtttga tcgcatcgaa tcacatgcaa tcagcgcggg agtgctgcgc
    12841 cgagtcgctg tgttgcgctc atgtcacccg ggccggaaaa gaccggccct ccttctttac
    12901 ttcgctccac acaacgaccc gtcacagcac gcctgcaacc accgaaacgg tcggggattg
    12961 aaagccttcg catgcggcgt tggtgccggc tgccgagggc gtggggtgat tggcacagcg
    13021 aagtaaagga ggaggttcgg ccgcaggccg atgccggggg acatgagcgg agccggtcgc
    13081 cccgcgccct cggcagcacc gcgcgcaagc ctgcgtcggg accctgtgcc gactttagcc
    13141 ttgcaccagc cgccggctct tcgcgatcga catcaggatg ccgagcccca ggcccaggat
    13201 caccatcgcg gttccgccgt agctgatgaa gggcaacggc acaccgacca ctggcaggat
    13261 gccgctgacc atgcccatgt tgacgaaagc gtaggtgaag aagctcaacg tgatcgagcc
    13321 ggccagcagg cgcgtgaaca cattgggtgc ctcggaggcg atcatcagcc cgcgcacgat
    13381 caggaacagg aaaccgagca gcaagacgac acaaccgatc agtccgaact cctcggagta
    13441 ggcggcaaag atgaaatcgg tggtgcgctc gggaatgaac tcgagatgcg tctgcgtgcc
    13501 cttcatgaag cccttgccgg tgatgccgcc cgagccgatg gcgatcattc cctggatgat
    13561 gtgaaaaccc ttgcccagcg gatcggtggt ggggtcgagc aaggtgcaga cccggttctt
    13621 ctggtagtcg cgcagcatcg gccacttcac atcgggttgg cagatcgcgt cgcccgaaag
    13681 gaccagcacc gcgatggcga tcaccgccac cgcgaccacg ggcgcgatca gcttccaggg
    13741 cagcccggcg aagaagatca cgaacaggcc ggccgccagc acgaggatcg cggtgcccag
    13801 gtcgggctgc ttgacgatca acccgaccgg caccgccagg atcagcagcg cgacgatgaa
    13861 atcgagcgcg cgcacttgtc cttcgcgacg ctggaaccac cacgccagca tcaacggcat
    13921 ggcgatcttg agaatctcgc tcggctggat cacgacgccc agatcgagcc agcgcgtggc
    13981 acccttcttg gtgatgccga tcagcgcggt ggcgaccagc agcgccacgc cgagcgcata
    14041 gagcggaacc gccagagcca tcaaccgctg cggagggatc tgggcgacga cgaaaagaat
    14101 gccggctgcc agcagcatgt tgcgtgcgtg gtcgacaaac cgggtgccat gatcgaaacc
    14161 ggccgaatac atcgtcgtca gcccgagggc cgccaggatc agcaccgcca gcagaagcgg
    14221 cccgtcgaaa ccgcgaaaca gcggaatcat ccgctgcagc agcgaaggct tctcgaatgc
    14281 cggcctcatc gcctgagcgc ccggttgtcg ggcgtttcgg ccgccgcgtt tgcagagacc
    14341 agcgacgccc cccctgaaac ggccatcggc gcggcagcga gcgaagttgc aggtgccgaa
    14401 gccacgccga tcatcgcggc agccgccgac ggggtactgc cgctgcccgg cagcaccaga
    14461 tcctgcacgt tgcgcggctg gccgatcggc gcggccgagg tgccggactt cgtcgctgcg
    14521 atgtcctcct cgctcggcac ccgacccagc agcatgaagt cgaacagcct gcgtgcgatc
    14581 ggtgccgcag cccccgcgcc ccagcccgcg ttctcgacga tcaccgccac cgcgatctgc
    14641 gggtcggcca gcggggcgaa agcgatgtag agcgcgtgat cgcgcaggtg ctcatcgacg
    14701 cggtgggcgc tgtacttctc gttctgcttc aggccgactg cctgcgccgt gccggtcttg
    14761 cctccgctct ggtagccggc tccggcaaac acacgcgccg aggtgccggc gatcgtgacg
    14821 ccattcatcg cctcgcggat cacatcgacc tgctcgggtt tcagctcgac gggctcgagc
    14881 gcctgcgccg agatcttgcg ctgcgcgcca gtcagcacgt cgtcgatctg gcgcaccagc
    14941 cggggctcgt agcgcttgcc ggcggacgac agcgtggcca tcgcggtggc catctgcagc
    15001 atcgtgaagc tgttgtagcc ctgcccgatg ccaagcgaaa tcgtctcgcc ggcgtaccac
    15061 ttcttctgtt ccggcttctt gtaggcacgc cgcttccact cggtcgacgg cagcaggccg
    15121 gtgacctcgc cttcaacgtc gatgcccgtc ttgcgcccga agccgaaggg cgacagctgg
    15181 tcgtggatca ggtcgacgcc catctcattg gccagcgtgt agaagtagac gttgctcgac
    15241 tcgacgatgg cacggcgcat gtccatgatg ccgcctcgct cgttctcggg gctgccgtat
    15301 cgatggccgc cgaatgtgaa gctgcctgtg tcgttgatca gcacgctggg cgagcgcttg
    15361 ttcgagttca gcgcggccat cgccatgaac ggcttgtagg tcgagcccgg cgggtaggtg
    15421 ccacgcagcg cccgattcag cagcggcttg tctggcgatt cgttgagttc cttccagctc
    15481 tcgctgtcga ttccatcgac gaagaggttc ggatcgaagg tgggcttgct gacgaacgca
    15541 agcacctcac cgttgcgcgg atcgatcgcg accaaagcgc cgcgccgatc accgaacagg
    15601 gtctcgacca gcgcctgcag gcggatgtcg atcgaaagga ccacggtgtt gccgggggtc
    15661 gccgggttgc tgcgcaggcg ccgcacggca cgcccggagg cactggtctc gacttcctcg
    15721 aaaccggtgg tgccatgcag gtcgctctcg tagctctgct cgatgccgag cttgccgatg
    15781 tactcggtgc cacggtagtt ggccgcgtcc tccagctcgt cgatgacttc cttctcggcc
    15841 tggttgatgc ggccgatgta gcccagcaga tggctgccga catcgccgag cgggtagttg
    15901 cgaaacagcc gggccttcac gtcgacaccg gggaagcggt agcgctgcgc ggtgaagcgg
    15961 gcgacttcct cgtccgtgag cttggtgcgg atcggcagcg attcgaagtt cttgctttcg
    16021 tccatcagcc gcttgaaacg cttgcgatca cgggtctgta tgtcgatcac ctccgagagc
    16081 tggctgatcg tcgactcgag gtcttgaacg cgcgccggca tgatctcgag cgtgtacgcc
    16141 gaatagttgt tcgcgagcac gacgccgttg cggtcgacga tcaacccgcg gttcggaacg
    16201 atgggtacga ccgagatgcg gttgttctcg gcctgcacgc tcagttcgtc gtgcttcacg
    16261 atctgcaggt gaaccaggcg gtagcccagc aggccgaaac acagcagcac gaaggcggtg
    16321 gcggccacca gccgcgcatg gaaccggctg agttcctgct cgacgttcct gatctcagtc
    16381 actggcgcct cctcggggga gtggaggcag acgcgccgtc cccggctgct cggagacgcc
    16441 cgccggaatg cccagaggcc tggccagcct ctgggcggca gcgacagcga cgtggaagct
    16501 cacaaaggtc gattctggtc gcggtctggc gcgcggcgct gcggcgcgag cagcaaccag
    16561 gtggcaagcg gccacagcaa cgcctcgaat gccggtgcca gaagcgcctg ccagcctggc
    16621 agcatgccac cggcgatcat ccgcaccgcg aacgaaacca ggtgcgcagc cacgaacagc
    16681 ggcaggatct ggagtgcctg cgacggcagg gtgaaccaca gcagccggcg atggatcgtg
    16741 gtcgcgacga agcccagcag cgtgtacgcg agcgcatgct gcccgaggat ggcgccatcg
    16801 tggacgtcca tcaccaggcc gaacatgaag gccacgccga cgctcacccg acgcacctga
    16861 tgcacgttcc agaacaccag cgcgagcgcc agcaggtcgg gcgcagcggg atggcgaccc
    16921 aacggcacca gattcagcgc gagtgccgtc aacagagtga accacaggaa cagcgggttg
    16981 accggcaaca gcaactgatc ggagcttcgc ggcatgatca tcggcgggct cctttcctgg
    17041 caggcgcgga agccgaggac gccgcagcgg tgggctcgtc cacattcggc tccggtcgtg
    17101 gcggcaactg cagcccgatc ggttccagca caagcacgtg gcgcacgccg tccggcagag
    17161 ccgccggaac cagcatgatc ttcgcgaacc ccgagtcgac cttgcgatcg accgtcacca
    17221 ccctggccac cggcagtccg ggcggataga caccatcgac gcccgacgtc tgcagcaggt
    17281 caccgacctg cacatccgcg ttgccggcca tgaaccgcag ttccagcgag tctccggtcg
    17341 caccaccgaa cgccgcgctg cgcgccatgg tgcgcgcgtt gagcaccgga acggccgcat
    17401 ccttgtcggt cagcaacgtg acttcggacg acagcggata ggcccgtgtg acctgcccga
    17461 tcactccggc atcgctgaca accggcgagc cggcaacgat gccttgcgcg agcccctgat
    17521 cgatgaccac cttgcgggag tacggatcgg tcgattcgta caacacctgc gctgcgcgcg
    17581 agcgcaccgt gagtccggct ctcagttcaa gcagggtgcg cagccgctcg ttctcggctt
    17641 gaagttgatt cgcacgggcc acgtgaacgg cctgagccgc gagttcacgc cgtgcggccg
    17701 attcagcagc aatggcatgc tgcagaccgc cggcgtagtc ggctgcctgc tccgaccagt
    17761 cgaccggcgc cagcagcgtg cgctggatcg ggtgcagcgc agtggccagc agcgcacgca
    17821 gcggctgggt cagcgtgaat cgggtgtcag ccaccatcag gaacacagcc agcgccgagc
    17881 acagggccag cttggtcagc gcagacgggc cttgccggaa gaaaggcggc ggggttcgat
    17941 ccagggtgcc taagggcatg ggtggatgcc gcaagcaacg aacacggcga aggcccccgg
    18001 agtcacgctg cagccacccg ccctggcgtc actcggaagt gaagatggag ccgaggcgtt
    18061 ccatgcgctc cagcgccatg ccacagccgc gaaccacgca ggtcagcggg tcttcggcga
    18121 ccagcaccgg cagcccggtt tcctcggcga gcaggcgatc caggtcgcgc agcagcgc
//
LOCUS       DAOORV010000070        18175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3746_length_18175_cov_8.525166, whole genome shotgun sequence.
ACCESSION   DAOORV010000070 DAOORV010000000
VERSION     DAOORV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_18175_cov_8.525166"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKV56_10630"
     CDS             complement(<1..742)
                     /locus_tag="PKV56_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOX68311.1"
                     /translation="MTADHAPSAPTSPAAADENQLITERREKLAAIRAQGVAFPNDFK
                     PEHRAADLVDAHGERSNEELEPLNITVSVGGRMMLKRVMGKASFCTLQDGSLGATGGR
                     IQLFVTKDALGDAGYESFKHLDLGDILGAEGSLFRTKTGELSVRVTTLRLLTKSLRPL
                     PDKFHGMSDQEQKYRQRYVDLITDDTARARFAARSKALSSIRQFMVDHGFMEVETPML
                     HPIPGGANAKPFVTHHNALDQQMFLRIAP"
     gene            899..1501
                     /locus_tag="PKV56_10635"
     CDS             899..1501
                     /locus_tag="PKV56_10635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX68312.1"
                     /translation="MPLPDPSLIHPTAVVDQGAQLGARTRVSHFAHVCAGARIGADCA
                     LGQGVYIGDEVSIGRNVNIQNNVSVYEAVTLEDDVFCGPSVVFTNVFNPRAAVERKAE
                     LRNTLIRQGATLGANCTVVCGTTIGRHAFVGAGAVVSRDVPDYALVVGVPARQIGWMS
                     RHGEPLALPLKGDGSATCPATGERYVLRQGVCALASEAAA"
     gene            1601..3046
                     /locus_tag="PKV56_10640"
     CDS             1601..3046
                     /locus_tag="PKV56_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOX68313.1"
                     /translation="MWYGAAVLIERALGLLLLPLLTRRLSEAEYGIWAQAAVVSSVLM
                     PLVLVCLPTGIVRFCSAGIDIAQRRLWVRRSLLLATGLWGLLALAGWLLPAHASEMVF
                     GAATHARFVTVALVLLAADVLFDLLVAYLRATFRMRSISMLLVVRGSMRFGLMLFALG
                     PLALSFELAFELLAAFQLVLVSAGFMVEYFRRSPRQPVSGGGVENTPVTWRTMIGFSA
                     PLVLMSVLSSAHAYTDRFVITHQLGLEATAVYAAVTTVVSVVSVAYTVLGFTLFPVLS
                     RLWTQGDQPQATELVAKVTGVFLFIALPFVFWLVCVSARLIPLLTTGAYRAQADVVLM
                     LGLAAVSFGLYQIVLYLLLLAGQGSRVVVIVLSATLVNAALNWLLVPRFGLSGAAAAA
                     ALSNALLAVIAYAWAWRCSKVRFPWAGAVRIAMGAMLAALVSQMMAPWFETMQWVGLM
                     LSLTVVYLVHGIVDVALPWSLIRAQPRRSAR"
     gene            3057..3740
                     /locus_tag="PKV56_10645"
     CDS             3057..3740
                     /locus_tag="PKV56_10645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX68314.1"
                     /translation="MNPTSPNRFDQQYSLDGRDYLEHLDFYTWTRHFHVFKDVATLVT
                     GDLLEVGTGDGVVRRCSEPFLRSTVVMDINPRLKPDVVGNLLDSQPALEGRFDAVVCT
                     EVLEHIPFNLLPRCLAHLHSFLRPGGMLFLTLPHRKGHMLVVTPRQRLVKWRFPVGLT
                     SLGEAYNRFVRRRIWIDPHHCWEIGDGHVRRSQIDAALLAAGLHTRKFTELPYCDYWV
                     LQRPAMDPA"
     gene            3737..5062
                     /locus_tag="PKV56_10650"
     CDS             3737..5062
                     /locus_tag="PKV56_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX68315.1"
                     /translation="MKVLLISYYYPPMGGAGVQRALKLSKYLGDFGVEPVVLAGHDPG
                     YTRDESLLAEVPAGVRVLRIEHRSLMQRLLARRAATVAPRSAAAPAAASRSPWLRDTL
                     LGAYAATRFPDDQAGWAKRAFEPALALLRDERIELIFSTAPPMSAHALAARLARRSGL
                     PWVADYRDLWTDNPGYLAPGWRRAIDQRTEAAWLKRAAGVVTVTPSWQRQFAQRLGAA
                     CPVVCIPNGYDEADFERLPTERRSDGAFRLVHTGSFYGPRDPGTLLDGVAAYLRSAAP
                     SARPLVLRLVGQMGSRFAERLARFDAEHPGVVEHRPYVPHHEAIAEMQSADALLLVVG
                     AGQGSKHGAVVAGTLPGKLFEYLRAGRPILLLGDEQGDAAQIVRHHAAGWIADEASPE
                     RIALALHQMMQPGPAPLPPATARADAFERRALAGEMAQFLRQRQVAHRG"
     gene            5055..5744
                     /locus_tag="PKV56_10655"
     CDS             5055..5744
                     /locus_tag="PKV56_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX68316.1"
                     /translation="MGELDRIEARYRERDASASLSGFWSLRNPVVLHLAQERERAVLR
                     ALSVGRVQLSELKLLDIGCGLGVEFASYLRWGSRVGQLFGIDLMGDRLRIALRDSPAE
                     VAQASGAALPFSDGAFDIVCQNVVFSSIVDDELRRATAAEMRRVLRSGGWVLWYDAAR
                     TRGRDPHFRPVPRNEVESLFPGVRWHWQRLSSDLGLLRRVADVLGEPGMQALDATGLF
                     RTHLLGLGRLA"
     gene            5741..6823
                     /locus_tag="PKV56_10660"
     CDS             5741..6823
                     /locus_tag="PKV56_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68317.1"
                     /translation="MTRHLCLLGDANSVHVRRWAGEMLGRGWRVSVVTARPEPIAGVE
                     QRMLAPVRHSADWLLRANQARETVAELAPDIVHAHYVTSYGYLAARCGQHPIVMTAWG
                     SDILLTPRRSALLRWLTGWTLRRADLITGDSLDLLDAMRVYRPTAPLRQIHWGADLAR
                     FSPSAWDLKPGFQIASLRSWEPNYSIDVIVRALALLARTRSGAGAHLHLLGGGSQEEA
                     LRALVRSLGLDGQVTFHGRLDDAGMSAVMQACKVSVSVPVSDATSVSVLESMACGLPV
                     VATDLPANRQWLEAGDGALVPAGDPLALAAALQAWCEDEDRARCIGERNRRRMEAEGA
                     RKVQMDAMSSLYDQLLRGDLPRAASD"
     gene            6826..7854
                     /locus_tag="PKV56_10665"
     CDS             6826..7854
                     /locus_tag="PKV56_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX68318.1"
                     /translation="MAGRCISVIVPCRNERAHIAAFCDGVLRQTLPQGWEMQVLIADG
                     QSSDGTRELLSALSADDGRIQLLDNPQRIVSTGLNRAVDSARGEVIVRMDVHTSYAPD
                     YVAQCLATLEATGASCVGGPWRPVGEGWPQAAIALAFQSRFGSGGAASRRVDFSGPVD
                     TVYLGAWRRDELLRLGGFDESLVRNQDDELNLRIVRAGGVVWQSAAIRSTYTPRASFS
                     ALFQQFQQYGYWKVPVIRKHRLPASPRHLAPFAFVAGLMLLAALGLAWAPARGALVGV
                     LSVYAVAALSSAAALARKPHRLRDVLGVAWAIACMHMGYGLGFGRALLDVVVLRRAPG
                     AAATRLTR"
     gene            7851..8588
                     /locus_tag="PKV56_10670"
     CDS             7851..8588
                     /locus_tag="PKV56_10670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX68319.1"
                     /translation="MTIPVAMTMREPSEDEPAAVAERYARREARGGVGDLYSPLRPEV
                     WQARQERQREMLALLARHALLPLHQLRVLEIGCGSGSNLLELASLGFDAARLVGNELL
                     PERAAIARRQLPAATALHEGDASKLPFEAGSFDVVYQSTVFSSLLDDAFQQRLADAMW
                     RWTRPGGGVLWYDFTWDNPGNPDVRGVPLKRVRALFPRGRIDAARVTLAPPLSRRVVR
                     VHPGAYRLLNALPFLRTHLLCWIGKPR"
     gene            8585..9763
                     /locus_tag="PKV56_10675"
     CDS             8585..9763
                     /locus_tag="PKV56_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOX68320.1"
                     /translation="MTTPPSFIPFALPDIGDDEIAEVVDTLRSGWITTGPKTRRFEED
                     FAAFLGPSAAGEPDLQAMAVNSATAGLHLALEALGIGPGDEVITTTHTFTATAEVVRY
                     LGADVVLVDIDPATLCIDVAAVDAAITPRTKAVMPVHYAGLAADMTALLQVARRHGLK
                     VVEDAAHALPTTCGGRLIGTLASDVTVFSFYANKTMTTGEGGMVVTRDADLARRIQVM
                     RLHGINRDAFDRFTAKAPSWYYEIVAPGFKYNLTDIASAMGLHQLRRARGFQVTRAAI
                     AARYDAAFADLPVLVPPQPMPGDTHAWHLYVLRLTDEASLTRDALIDGLYAAGIGCSV
                     HYIPLHLQPYWRDRYGLVPERFPHSQHAYERMLSLPLHTRMAPHEVQRVIDAVRSLLG
                     "
     gene            9781..10401
                     /locus_tag="PKV56_10680"
     CDS             9781..10401
                     /locus_tag="PKV56_10680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOX68321.1"
                     /translation="MAKRLFDLVASGLGLLLLVPLLLLMALAIKLDSPGPVFFRQARV
                     GRHGVEFRIHKFRTMLHFERSGSAGPQSAVAQLTVGADPRITRVGALLRRTRLDELAQ
                     LIDVLCGNMSLVGPRPEVPRYVATYPRDLRDKVLSVRPGITDPASLAFRDESAMLACA
                     ADPQREYIEIVLPAKLRLSARYIDEASFGTDLRLIAATLRTLWIGR"
     gene            10449..12368
                     /locus_tag="PKV56_10685"
     CDS             10449..12368
                     /locus_tag="PKV56_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOX68322.1"
                     /translation="MLWHRLDRTLARCRPHRKLLTLLFDALVVAICWNFTYLFRLGFD
                     RWWSARPGYDVWVMGAVVVTYVLVFILAGVPRGMWRFSGFGEVKRLTLSCGIAGALCA
                     SGVLMAQLQAVPRAVLAVHPFVTLMGLCMTRIAYRMLYEHARAQITGGHGEVRRAIVM
                     GAGEAARLLVAGLPQQGWTVLGLLDDDPAKRGARISGAPVLGTLADVIDPRITAGATH
                     IITALPGTTAADRRRAIGLAAATTLPVLTVPSVSELQSGATRIERIRAIEPEDLLGRE
                     PVVLDEAGIAELVCGKTVLVTGAGGSIGSELCRQLARYGPARLVLYELSEFALYQIEQ
                     QLGELHPGLPLVRLMGDVKDLPHLRATCNSYRPHLVFHAAAFKHVPLMEDENAWAALQ
                     NNTLGTHHAALAAMECGAERFVMISTDKAVNPTNVMGASKRAAERVLSHLATQGTATV
                     FSAVRFGNVLGSSGSVIPKFKEQIAKGGPVTVTHPDITRYFMTIPEAVRLVLQSAVLA
                     RSGQVYVLDMGEPVKIVELARDLIRLSGQDENEIAIVFSGLRPGEKLFEELLADADLT
                     QPSAHPRLRIAKLNDESTDIWTRELLEWLGSRSGGSSPASQRERLAHFIPEYKPKKII
                     DRRALSAESPAISAP"
     gene            complement(12334..13425)
                     /locus_tag="PKV56_10690"
     CDS             complement(12334..13425)
                     /locus_tag="PKV56_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68323.1"
                     /translation="MSFLLLSFVVSLLVTLAVVRSSARHGRLSSDTEMSGPQKFHSDP
                     VPRIGGVGVLVALVAGAALAHFMRPAHSLSLWLLLAASLPTFLGGFAEDMTKNVSAAR
                     RLTATVVSAGLAVWLLDAVIHRTDIPGVDQLVAIEPFAIALTIFVVTGVANSVNIIDG
                     FNGLASMCVLIMMLAVAYVALQVGDRLVLTAAMIAAGAVLGFFVWNFPGGLIFLGDGG
                     AYLLGFVLSELGVLLIVRNSLVSPLFPLLLCAYPIFETIFSIYRRRVLKGVASSQPDG
                     IHLHTLIHRRLVRQLIGDTHEHRLRARNSMTSPYLWLLCSLSVIPAVAWWNNTAALSV
                     FIVLFMISYVALYWRIVRFKVPKWLVTRR"
     gene            complement(13508..14044)
                     /gene="rfbC"
                     /locus_tag="PKV56_10695"
     CDS             complement(13508..14044)
                     /gene="rfbC"
                     /locus_tag="PKV56_10695"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOX68324.1"
                     /translation="MKVTPTALDGVLILEPRVFGDERGFFLESFNLQTFQAAVGHDVT
                     FVQDNHSRSTQGVLRGLHYQRDPHAQGKLVRVTQGSVFDVAVDIRENSPTYGQWVGVE
                     LSGRNHKQLWIPPGLAHGFLVTSDSADFLYKTTAYYRPDSEGCIRWDDPNLAIPWPLD
                     GRTPSLSAKDAAAPMLRS"
     gene            complement(14044..14934)
                     /gene="rfbA"
                     /locus_tag="PKV56_10700"
     CDS             complement(14044..14934)
                     /gene="rfbA"
                     /locus_tag="PKV56_10700"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOX68325.1"
                     /translation="MTTTQRKGILLAGGSGSRLHPATLAISKQLLPVYDKPMVYYPLS
                     TLMLAGIRDILLISTPQDTPRFESLLGDGHRWGVNISYCVQPSPDGLAQAFVLGRNFV
                     DGHPSALVLGDNIYHGHDLQGLLQAAHARTEGASVFAYHVQDPERYGVVAFDKSKRAL
                     SIEEKPKVPQSNYAVTGLYFYDAQVCDIAASIRPSARGELEITDVNARYLAQGQLNVE
                     IMGRGYAWLDTGTHDSLLEAGQFIATLEKRQGLKVACPEEIAFRSGWIDAAQLEALAQ
                     PMLKNGYGQYLMRIVNNQVF"
     gene            complement(14957..15850)
                     /gene="rfbD"
                     /locus_tag="PKV56_10705"
     CDS             complement(14957..15850)
                     /gene="rfbD"
                     /locus_tag="PKV56_10705"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOX68326.1"
                     /translation="MKILLFGKDGQVGWELQRALAPLGQVIALGSRNTEFAADFSDPD
                     ALAATVRAVAPRWIVNAAAYTAVDRAESEPELARTVNAIAPGVLAREAAVIGASLVHY
                     STDYVFDGSGSTPWVEDAPTGPLGVYGTTKLEGEEAIRRSGCPHLILRTSWVYAARGS
                     NFAKTMLKLAAERAALKVIDDQIGAPTGADLLADVTAHALRQAGRQPQVCGTYHVAAS
                     GLTSWHAYAVHVIEQARCLGRPIRVEREAIEPVPTSAFPTPARRPGNSRLDTRKFQQA
                     FDLNLPLWQTGVDRMLAEVLG"
     gene            complement(15847..16914)
                     /gene="rfbB"
                     /locus_tag="PKV56_10710"
     CDS             complement(15847..16914)
                     /gene="rfbB"
                     /locus_tag="PKV56_10710"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOX68327.1"
                     /translation="MILVTGGAGFIGANFVLDWLDQSREPVLNLDALTYAGNRESLRS
                     LDGDARHVFVHGDITDRALLDALFTTHRPRAVVHFAAESHVDRSIHGPGAFIHTNVQG
                     TFTLLEAARAFWSTLPPAEQGEFRFHHVSTDEVYGSLGPADAPFTETHLYEPNSPYSA
                     SKAGSDHLVRAWHHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDG
                     RQVRDWLYVKDHCAAIRAVLARGRIGETYNIGGWNEMPNIDIVHTVCALLDEMRPDPA
                     GPHGRLITHVTDRPGHDRRYAIDARKLESELGWKPAETFDTGIRKTVRWYLDHADWVS
                     RVQSGAYRAWVDTNYAARTAG"
     gene            17038..>18175
                     /locus_tag="PKV56_10715"
     CDS             17038..>18175
                     /locus_tag="PKV56_10715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX68328.1"
                     /translation="MTETPQENTSGAPATEAVAAPERFDTLALDAKLLRAVADSGYTQ
                     MTPIQAKAIPIVLDGRDVMGAAQTGTGKTAAFTLPLLQKMLKHENSSASPARHPVRAL
                     VLAPTRELADQVANNVKAYAKHSKLRSMVVFGGIDMKPQTAELKTGVEVLIATPGRLL
                     DHIEAKNCNLGQVEYVVLDEADRMLDIGFLPDLQRILSYLPKQRQTLLFSATFSPEIK
                     RLAESYLQDPILVEVARPNATATNVEQRFFSVSNDDKRAAVLKIIRERTLTQALVFVN
                     SKLGCARLARSFERDGLRTNALHGDKSQDERLKALEAFKRGEVDVLVCTDVAARGLDI
                     ADLPAVFNFDVPFNAEDYVHRIGRTGRAGASGLAVTLVSRDDTRQ"
BASE COUNT         2794 a         5839 c         6225 g         3317 t
ORIGIN      
        1 cgggcgcgat gcgcaggaac atctgctggt cgagcgcgtt gtggtgcgtg acgaacggct
       61 tcgcgttggc gccgcccggg atcgggtgca gcatcggcgt ctcgacctcc atgaagccgt
      121 ggtcgaccat gaactggcgg atcgagctga gtgccttgct gcgcgctgcg aagcgcgccc
      181 gggcggtgtc gtcggtgatc aggtcgacat agcgctgccg gtacttctgt tcctggtcgc
      241 tcatgccgtg gaacttgtcg ggcagcggcc gcaggctctt ggtcagcagg cgcagcgtgg
      301 tcacgcgcac cgagagctca ccggtcttgg tcctgaacag cgagccctcg gcgcccagga
      361 tgtcgcccag gtcgagatgc ttgaacgatt cgtagccggc gtcgccgagc gcatccttcg
      421 tgacgaacag ctggatgcgg ccgccggtgg caccgagcga gccgtcctgc agcgtgcaga
      481 agctggcctt gcccatcacc cgcttgagca tcatccggcc gcccacgctg acggtgatgt
      541 tcagcggctc gagttcctcg ttgcttcgct cgccatgcgc gtcgaccagg tcggctgcgc
      601 ggtgttcggg cttgaagtcg ttcgggaacg cgacgccctg cgcgcggatc gcggccagct
      661 tctcgcgacg ttcggtgatg agctggttct cgtcggcggc tgccggtgaa gtcggggcgg
      721 aaggggcgtg gtctgcggtc atgggctggg tccgggaggt cgtcgatttt aggttgcgcc
      781 ctgccgcctg tctgcggcgc aggcgtttgg gcgggctccc ctaaaatcac gctcatgcag
      841 aaacccgttg tccccagttg cgcgcgatga cccctcgcgc gcgcacccct ggtttgccat
      901 gccgctgccg gaccccagct tgattcatcc gacggcggtg gtcgatcaag gcgcccagct
      961 cggggcgcgt acgcgggtgt cccacttcgc gcatgtgtgc gcaggagccc gcatcggcgc
     1021 cgactgtgca ctggggcagg gcgtgtacat cggcgatgag gtcagcatcg gccgcaacgt
     1081 caacatccag aacaacgtgt cggtctacga ggcggtgacg ctcgaggacg acgttttctg
     1141 cggcccgagc gtcgtcttca ccaacgtctt caatccgcgc gccgcggtcg agcgcaaggc
     1201 cgaactgcgc aacaccctca tccgtcaggg agccacgctc ggcgccaact gcaccgtggt
     1261 gtgcggcacc acgatcggcc gccatgcatt cgtcggcgct ggagccgtgg tcagtcgcga
     1321 cgtgcccgac tacgcgctgg tcgtcggcgt gccggcgcgc cagatcggct ggatgagccg
     1381 tcacggcgaa ccgctcgcgc tgccgctgaa gggcgatggc tccgccacct gcccggcgac
     1441 cggtgagcgc tacgtgctgc gccaaggcgt gtgtgctctg gcgagcgaag cggccgcctg
     1501 atcctgttcg ggctgcggtc cgcagcctgg agacagtggc ggctgtgcgc cgagacattc
     1561 gctcgggcct gatttgaagg tcgatctgaa atccgacacg gtgtggtacg gcgccgccgt
     1621 gttgatcgag cgggccctcg gactgttgct gctgcccctg ctgacgagaa ggctcagcga
     1681 ggccgagtac gggatatggg cgcaggcggc ggtggtgtcc agcgtgctga tgccgctcgt
     1741 gctggtctgt ttgccgaccg gcatcgtccg tttctgctcg gcaggcattg acattgccca
     1801 gcggcggctg tgggtgcgcc gctcgctctt gctggcgacc ggtctgtggg ggctgctggc
     1861 cctggcgggc tggctgctgc ctgcccatgc gtccgaaatg gtattcggtg cagcgacgca
     1921 cgctcggttc gtcacagtcg ctcttgtcct gttggctgcc gacgtgctgt tcgatctgct
     1981 cgtggcttac ctgcgagcca cattcaggat gcgcagcatc tctatgctgc tggtcgtgcg
     2041 agggagcatg cgattcggtc tcatgctgtt cgcactcggt ccgctcgcgc tgtctttcga
     2101 gttggctttc gaactgctcg ctgcgttcca actggtcctg gtctcagcgg gtttcatggt
     2161 ggagtacttt cggcgatccc cccgacaacc agtttcaggc ggtggcgtag agaacacgcc
     2221 cgttacctgg cgaacgatga tcgggttctc cgcgccgctg gtgctgatgt cggtgctgtc
     2281 ttctgcccac gcgtataccg accggttcgt gatcacacac cagttgggac tcgaggcaac
     2341 tgccgtctac gccgccgtca ccacggtggt ctcggtcgtg agcgtggcat acaccgtgct
     2401 cggattcacc ctgttccccg tactgtcgcg tttgtggact caaggcgatc aacctcaggc
     2461 gacagaactt gtggccaagg tgaccggagt gttcctgttc atcgcgttgc cattcgtttt
     2521 ctggctggtg tgtgtgtccg ctcgcctgat acctctcctg accaccggcg cgtatcgtgc
     2581 gcaggccgat gtggttctga tgctcggcct ggctgcggtg agcttcggcc tgtatcagat
     2641 tgttctttac ctgctcctgt tggctggaca aggttcgcga gtcgtcgtga ttgtgctgtc
     2701 ggcgacattg gtgaatgcgg cgctgaactg gctgcttgtg ccgcggttcg gcttatctgg
     2761 cgcggccgct gctgcggccc tttcgaatgc gctgctggcc gtgattgcgt acgcatgggc
     2821 atggcggtgt tcgaaggtgc ggtttccgtg ggcaggagcg gttcgcatcg ccatgggtgc
     2881 gatgctcgcg gcgctggtgt cgcagatgat ggcgccgtgg ttcgagacaa tgcaatgggt
     2941 cggactgatg ctcagtctca ccgtggtgta cctagtccac ggtatcgtgg atgttgctct
     3001 gccgtggtca ttgatacgcg cccaaccccg gcgctctgcc agatgatgga attccgatga
     3061 accccacgtc gcccaatcgc ttcgatcagc agtactcgct cgatggccgg gactatttgg
     3121 aacacctcga cttctacact tggactcgcc atttccacgt gttcaaggat gtcgcaaccc
     3181 tcgtcacggg cgacctgcta gaggtcggca ccggcgatgg cgtggtccgc cgttgttccg
     3241 agccgttctt gcgcagtacc gtggtgatgg acatcaaccc ccggttgaag cctgacgtgg
     3301 ttggcaacct gctcgactca cagcctgctc tcgaaggacg cttcgacgcg gtcgtctgca
     3361 ccgaggtgct agagcacatt cctttcaatc tgctcccgcg atgcctcgcg cacctgcatt
     3421 cgttcctgcg ccccggtgga atgctgttcc tgacgctgcc gcatcgcaag ggacacatgc
     3481 tggtcgtgac gccacggcag cgtctcgtga agtggcgctt cccggtcgga ctgacgagcc
     3541 tcggcgaggc ctacaaccgc tttgtgcgtc ggcgcatctg gatcgatccg caccactgct
     3601 gggagatcgg cgacgggcac gtgcggcgtt cgcagatcga tgccgccttg cttgccgccg
     3661 gcctgcacac ccgcaagttc accgagctgc cctattgcga ctactgggtg ttgcagcgtc
     3721 ctgcgatgga tcccgcgtga aggtactgct catcagttac tactacccac cgatgggcgg
     3781 ggccggggtg cagcgtgcgc tgaagctcag caagtacctc ggcgatttcg gagtcgagcc
     3841 cgtggtgctg gccgggcacg acccaggcta cacgcgcgac gaatcgctgc tggccgaagt
     3901 tcctgcgggc gttcgggtgc ttcgcatcga acaccgctcc ctgatgcagc ggctgctggc
     3961 ccggcgagcc gcgacggtcg cgccgcgatc cgctgcggca cccgcggcgg cgagccggag
     4021 cccctggctg cgcgacacgc tgctcggggc ctacgccgca acccgtttcc cggacgacca
     4081 ggccggctgg gcgaagcgcg ccttcgagcc ggcgctcgct ctgctgcgcg acgagcgcat
     4141 cgagctgatc ttcagcaccg ctccgccgat gtcggcgcac gcgctcgcgg cgcgtctggc
     4201 tcgccgctcg ggcctgccgt gggtcgccga ctaccgcgac ctgtggaccg acaaccctgg
     4261 ttacctcgcg ccgggctggc ggcgtgcgat cgaccaacga acggaagcag cgtggctcaa
     4321 gcgtgcggcc ggtgtagtga cggtcacgcc gagctggcag cggcagttcg cgcagcgtct
     4381 cggggcggct tgtccggtgg tgtgcatacc gaacggctac gacgaggccg atttcgaacg
     4441 gctaccgacc gaacggcgca gcgacggcgc gttccggctg gtgcacacgg gcagcttcta
     4501 cggtccgcgc gaccccggca cgctgctcga tggcgtggct gcgtatctgc gttcggcggc
     4561 gccatcggcg cgaccgctgg tgctgcggct ggtgggccag atggggtcgc gctttgccga
     4621 gcgtctggcg cgcttcgatg ccgagcaccc cggcgtcgtc gagcatcgcc cgtacgtgcc
     4681 gcatcacgaa gcgatcgccg aaatgcagtc cgctgatgcc ttgctgctcg tggtcggggc
     4741 cgggcaaggg tcgaaacatg gtgcggtcgt ggcaggcacc ctgccgggca agctcttcga
     4801 atacctgcgc gcaggtcggc cgatcctgct gctgggggac gagcaggggg acgcggcgca
     4861 gatcgttcgg caccacgctg ctggctggat cgcagatgag gccagccctg agcggatagc
     4921 gctggctttg caccaaatga tgcagccagg cccggcgccg ctgccacccg ccacggcgcg
     4981 agccgacgct ttcgagcgcc gtgcactggc tggagagatg gcgcaattcc ttcggcaacg
     5041 tcaggtggcg catcgtgggt gagctcgacc ggatcgaggc acgttaccgc gagcgggatg
     5101 cctctgcgtc actgtccggc ttctggagcc tgcgcaaccc ggtcgtgttg catctcgctc
     5161 aggagcgaga aagggcggtc ctgcgtgcgc tttccgtcgg tcgtgttcag ttgagtgagc
     5221 tgaaactgct cgacatcggc tgcggtctgg gcgtcgagtt cgccagctat ttgcgctggg
     5281 gcagccgtgt gggccagttg tttggaatcg atctgatggg cgatcggctg cggattgcac
     5341 tcagggatag tccagccgag gtggctcagg cctcgggtgc ggcgttgcca ttttccgacg
     5401 gtgccttcga catcgtgtgc cagaacgtgg tgttcagctc gatcgtcgac gatgagctac
     5461 ggcgtgcaac tgcggccgaa atgcgccgtg ttttgcgctc cggcggctgg gtactctggt
     5521 acgacgcggc gcgaacgcgt ggccgagatc cccacttcag gcctgtaccc cggaatgaag
     5581 tcgagtcatt gtttccaggt gtgcgctggc actggcaacg tctgagcagt gatcttgggc
     5641 tgctgcggcg cgtggccgac gtgttgggcg aaccaggcat gcaagcactg gatgccaccg
     5701 gtctctttcg cactcacctg ctcgggctgg gacggcttgc atgacgcggc acctgtgcct
     5761 gctcggcgac gccaacagcg tgcatgtgcg ccgctgggcc ggggagatgc tcgggcgtgg
     5821 ctggcgcgtc tcggtggtga cggcacgtcc ggagccgatc gccggcgtgg agcagcgcat
     5881 gctggcgccg gttcgtcact ctgccgactg gctcctgcgc gcgaatcagg cgcgcgagac
     5941 ggtggctgag ctggctcccg acatcgtgca cgcgcattac gtcacctcgt acgggtatct
     6001 ggccgcgcgc tgcggacagc acccgatcgt gatgacggca tggggcagcg acatcctgct
     6061 gacgccacgc aggagcgcac tgctgcgctg gctgaccggc tggacgctgc gtcgggcaga
     6121 cctgatcacc ggcgattcgc tcgacctgct tgatgcgatg cgggtgtacc ggccgaccgc
     6181 cccgcttcgg cagattcact ggggtgccga tctggcgcgt ttcagcccct cggcctggga
     6241 tctcaagccg gggttccaga tcgccagcct gcgctcctgg gaaccgaact acagcatcga
     6301 tgtcatcgtg agggcgctgg ccttgctcgc acgcacgcgc tcgggcgccg gtgcgcacct
     6361 tcacctgctc ggcggcggct cgcaggaaga ggccttgcgc gcgctggtgc gatcgctcgg
     6421 cctggatggg caggtcacat tccacgggcg actcgacgat gccggcatga gcgcggtgat
     6481 gcaggcgtgc aaggtgtcgg tgtcggtgcc cgtcagcgat gccacgtcgg tgtcggtgct
     6541 tgaaagcatg gcctgcggcc tgcctgtcgt cgcgaccgac ctgccggcca accggcagtg
     6601 gctcgaggcc ggtgacggtg cgctcgttcc tgcaggcgac ccactggcgc tggccgccgc
     6661 gcttcaagcc tggtgcgagg acgaggatcg agcccgctgc atcggcgagc gcaaccgcag
     6721 gcgcatggag gccgaaggcg cccgcaaggt tcagatggac gcgatgtcct cgctgtacga
     6781 ccaactgctg cgcggtgatc tgccgcgagc tgcgtcggat tgaccatggc gggccgctgc
     6841 atcagcgtca tcgtgccgtg ccgcaacgag cgcgcgcaca ttgccgcctt ctgcgatggc
     6901 gttctgcgcc agacgctgcc gcagggctgg gagatgcagg tgctgatcgc cgatggccag
     6961 agcagcgatg gcacgcgaga gctgttgtcg gcgttgagtg cggacgatgg gcgaatccag
     7021 ttgctggaca acccgcagcg gatcgtttcc accgggctca atcgagctgt cgattcggcg
     7081 cgcggtgagg tgatcgtgcg gatggacgtg cacacctcct atgcaccgga ttatgttgcg
     7141 cagtgcctcg cgacgctcga ggcgaccggc gccagctgtg tcggcgggcc gtggaggccg
     7201 gtcggcgagg gctggcctca ggccgcgatc gcgctggcct tccagagccg cttcggctcc
     7261 ggaggggcgg catcgcggcg cgtcgatttc agcggcccgg tggacacggt gtacctcgga
     7321 gcgtggcgcc gcgatgagct gctgcggctc ggcggcttcg acgaatcgct ggtgcgcaac
     7381 caggacgacg agttgaacct gcgcatcgtg cgagcggggg gcgtcgtctg gcaaagcgcg
     7441 gccatacggt cgacctacac gccgcgcgcc tcgttctcgg cgctgttcca gcagtttcag
     7501 cagtacggtt actggaaggt gccggtgatc cgaaagcatc gactgccagc gtcgccgcgc
     7561 cacctcgcgc cattcgcatt cgtcgccggg ttgatgctgc tggcggcgct gggactggcg
     7621 tgggccccgg cgcgcggcgc cctggtcggc gtgctttcgg tgtacgcggt ggcggcgctg
     7681 tcgagcgctg ccgcgctggc gcgcaagccg catcgtctgc gagatgtcct cggcgtggcc
     7741 tgggccatcg cctgcatgca catgggctac ggtctcggct tcggtcgggc gctgctggat
     7801 gtcgtggtgc tgcgccgtgc gcccggcgcc gccgccacgc ggctcacgcg ttgacaatcc
     7861 ccgtcgccat gacgatgcga gagcccagcg aagacgagcc ggcggccgtg gccgagcgct
     7921 acgcccgacg tgaggcgcgt ggcggcgtcg gtgaccttta cagcccgctg cgcccggagg
     7981 tctggcaggc tcggcaggag cggcagcggg aaatgctcgc gttgctggcg cgccatgccc
     8041 tgctgccgct gcaccaattg cgcgtgctcg agatcggttg cggcagcggc agcaacctgc
     8101 tcgaactggc cagtctgggg ttcgacgccg cgcgtctcgt cggcaacgaa ctgctgcccg
     8161 agcgtgccgc catcgcgcgg cggcaactgc ccgccgcgac cgcgttgcac gagggcgatg
     8221 cgtcgaagct tcctttcgaa gctggaagct tcgacgtggt ctaccagtcg acggtgttca
     8281 gctcgctcct cgatgacgcc ttccagcagc gactggccga cgccatgtgg cgctggacac
     8341 ggccgggcgg cggcgtcctc tggtacgact tcacctggga caacccgggc aatccggatg
     8401 tgcggggcgt gcccttgaag cgagtgcgcg cgctctttcc ccgcggcaga atcgatgcag
     8461 cgcgcgtcac gctggcgccg ccgttgagcc gtcgcgtggt ccgtgtgcac cccggtgcct
     8521 accggctgct gaatgcgctg ccgttccttc gcacccactt gctctgctgg atcggcaaac
     8581 cccgatgacg accccacctt cattcattcc ctttgccctt cccgacatcg gcgacgacga
     8641 gattgccgag gtggtcgaca cattgaggtc gggctggatc accaccgggc cgaagacgcg
     8701 tcggttcgag gaggatttcg ccgcgttcct cggaccgtcg gccgcaggcg agccggactt
     8761 gcaggcgatg gcggtcaact cggcgaccgc cgggttgcac ctcgcactcg aggcgctggg
     8821 catcggcccg ggcgacgagg tgatcacgac cacacacacg ttcaccgcca cagccgaggt
     8881 ggtgcgctac ctcggcgccg acgtggtgct ggtcgacatc gatccggcca cgctgtgcat
     8941 cgatgtcgcg gcagtcgacg ccgccatcac gccacgcacg aaggcggtga tgccggttca
     9001 ctacgccggg ctggcggccg acatgaccgc gctgttgcag gtggcccgac gacacgggct
     9061 gaaagtggtc gaggacgcgg cccacgcgct gccgaccacc tgcggtggac gactgatcgg
     9121 cacgctggcg agcgacgtca cggtgttcag cttctacgcc aacaagacga tgacgacggg
     9181 cgagggcggc atggtcgtga cgcgtgacgc cgatctggcc agacgcatcc aggtgatgcg
     9241 tttgcacggg atcaaccgcg atgccttcga tcgattcacc gcgaaggcgc cgagctggta
     9301 ctacgagatc gtggcgcccg gcttcaagta caacctgacc gacatcgcgt cggccatggg
     9361 cctgcatcag ctgcggcgtg cgcgtgggtt tcaggtcacc cgcgccgcga tcgccgcccg
     9421 ctatgacgcg gctttcgccg atcttccggt gctcgtgcct ccgcagccga tgccgggtga
     9481 cacccacgcc tggcatctct atgtgctgcg tctgaccgac gaggcatcgc tgacgcgcga
     9541 tgccctgatc gacggcctgt acgctgccgg catcggttgc agcgtgcact acatcccact
     9601 gcacctgcag ccctactggc gggatcggta cggcctggtg cccgaacggt ttccgcacag
     9661 ccagcacgcc tacgagcgga tgctgagcct gccgctccac acccgcatgg caccgcacga
     9721 agtgcagcgc gtcatcgatg cggtgcggtc cctgctgggt tgatgcgttc ggcacggtcg
     9781 atggcgaagc gactgttcga cctggtggcc tccggtctcg gcctgctgct gcttgtgccg
     9841 ctgctgttgt tgatggcgtt ggcgatcaag ctcgactcgc cgggtccggt cttcttccgg
     9901 caggcgcgag tgggtcggca tggcgtcgag ttccgcatac acaagttccg cacaatgctg
     9961 catttcgagc ggagtggtag tgccggtccg cagtcagcgg tggcgcagct cacggtcgga
    10021 gctgacccgc gcatcacccg cgtcggcgcg ctgctgcgcc gcacccggct ggacgagctg
    10081 gcgcagctga tcgacgtgct gtgcgggaat atgagcctgg tcggaccacg gcctgaagtg
    10141 ccgcgctacg tggcgaccta cccgcgggac ctgcgagaca aggtgctgag cgtgcgtccg
    10201 ggcatcaccg atccggcgtc actggctttc cgcgatgaat ccgcgatgct ggcgtgtgca
    10261 gccgacccgc agcgcgagta catcgagatc gtgttgcctg ccaagctgcg cttgtcggcg
    10321 cgctacatcg acgaggcttc gttcggcacc gacctgcgcc tcatcgccgc gacgctgcgt
    10381 acgctgtgga tcggtcgatg aggccgattg cgccgccgct tcgactatcg ccaacataat
    10441 ctgcctccat gctctggcac cgactcgatc gcaccctggc tcgctgtcgt ccgcaccgca
    10501 agctgctgac gctgctgttc gatgcgctgg tggtggcgat ctgctggaac ttcacttacc
    10561 tgttccgtct cggcttcgat cgctggtgga gcgctcggcc cggctacgac gtctgggtca
    10621 tgggcgctgt ggtggtcacc tatgtgctgg tgttcatcct ggctggcgtg ccgcgcggca
    10681 tgtggcggtt ctcgggcttc ggcgaggtca agcggctgac cctgtcatgc ggcatcgcgg
    10741 gggcgctgtg cgccagcggc gtgctgatgg cccagttgca ggcggtgcct cgcgcggtat
    10801 tggcggtgca tcctttcgtc accctgatgg ggctgtgcat gacacgcatc gcctaccgca
    10861 tgctctacga gcacgcacgg gcccagatca ccggtgggca tggcgaggtc cgacgcgcca
    10921 tcgtcatggg cgcgggtgag gcggctcggt tgctggtcgc cgggctgccg cagcagggct
    10981 ggacggtgct cggcctgctc gacgacgatc ctgccaagcg gggtgcgcgc atctccggtg
    11041 cgccggtgct tggcacgctg gccgacgtga tcgatccgcg catcacggca ggcgcgaccc
    11101 acatcatcac cgcgcttccg ggaacgacgg cggcagatcg gcgccgtgcg atcggactgg
    11161 ccgcggccac cacactgccg gtgctgaccg tgccttcggt cagcgaactt cagtcgggcg
    11221 ccacgcgaat cgagcggatt cgcgcgatcg agccggaaga tctgctgggg cgcgagccgg
    11281 tggttctgga tgaggccggc atcgccgaac tggtgtgcgg aaagacggtg ctcgtgaccg
    11341 gcgccggcgg gtccatcgga agcgaactgt gccgccagct ggcgcgatac ggcccggcgc
    11401 gcctggtgct gtacgaactg agcgagtttg cgctgtacca gatcgagcag caactcggtg
    11461 agctccaccc cgggctgccg ctggtgcgct tgatggggga cgtgaaggac ctcccgcacc
    11521 tgcgtgcgac ctgcaattcg taccggccgc acctggtgtt ccacgcagcg gccttcaagc
    11581 acgtgccgct gatggaagac gagaacgcct gggccgcatt gcagaacaac acgctgggga
    11641 cccaccacgc ggcgctcgcc gcgatggagt gcggtgccga gcgcttcgtg atgatcagca
    11701 ccgacaaggc ggtcaatccg accaacgtga tgggagccag caagcgcgcc gccgagcgcg
    11761 tgctgtcgca tctggcgaca cagggcaccg caacggtgtt ctccgcggtg cgctttggca
    11821 acgtgctggg ttccagcggc agcgtgatcc cgaagttcaa ggaacagatc gccaagggcg
    11881 gtccggtcac ggtcactcat ccggacatca cacgctattt catgaccatt ccggaggccg
    11941 tgcggctggt gttgcagtcg gcggtgctgg cgcgcagcgg gcaggtctat gtgctggaca
    12001 tgggagaacc cgtgaagatc gtcgaactgg cgcgggacct gatccgactc agtggccagg
    12061 acgaaaacga aatcgccatc gtcttcagtg gcctgcgacc cggcgaaaag ctcttcgagg
    12121 agctgctggc ggacgcggat ctcacgcagc cgtcggctca tccgcgcctg cgtatcgcca
    12181 agctcaacga cgaatcaacc gacatctgga cacgcgaact gctcgagtgg ctaggcagcc
    12241 gcagcggcgg cagttcaccc gcgagtcagc gagagcgcct tgcgcatttc attcccgagt
    12301 acaagccgaa gaagatcatc gatcgacgtg ccctcagcgc cgagtcacca gccatttcgg
    12361 caccttgaag cgcacgatcc gccagtagag ggccacgtac gagatcatga acagcacgat
    12421 gaacacgctc agcgcggccg tgttgttcca ccatgccacc gcaggaatca ccgacaggct
    12481 gcaaagcagc cagaggtagg gcgacgtcat cgaattgcgc gcccgcaacc ggtgctcgtg
    12541 ggtgtcgccg atcagctgcc gtaccagccg gcggtgtatc agggtgtgca gatgaatgcc
    12601 gtctggctgc gatgaggcga cgcccttgag gaccctgcgc cggtagatcg agaagatggt
    12661 ctcgaagatc ggatacgcgc acagcagcag cggaaacagc ggcgatacca gcgagttgcg
    12721 cacgatcagc agcaccccga gttcggacag cacgaagccc agcaggtagg cgccgccatc
    12781 gccgaggaag atcagcccgc cggggaagtt ccacacgaag aagccaagca ccgcgccggc
    12841 tgcgatcatc gcggcggtca gcacgagccg gtcgccgacc tgcagtgcca cataggccac
    12901 ggcgagcatc atgatcagca cgcacatcga ggccaggccg ttgaagccgt cgatgatgtt
    12961 gaccgagttg gccacgccgg tgacgacgaa tatggtcagc gcgatcgcga acggctcgat
    13021 cgccaccagt tgatccacgc cagggatgtc ggtgcgatgg atcacggcgt ctagcagcca
    13081 gaccgccagg cccgccgaaa cgacggtggc ggtcagtctt cgcgcagccg aaacgttctt
    13141 tgtcatgtct tctgcgaatc cgcccagaaa cgtgggcagg ctggcagcca gcagcagcca
    13201 cagcgaaagg ctgtgcgccg gccgcatgaa gtgcgccagc gccgcgccgg cgaccagcgc
    13261 gaccagcacg ccaacgcctc cgatgcgcgg caccgggtcg ctgtgaaact tctgggggcc
    13321 ggacatctcg gtgtccgagg acagtcgacc gtgccgggca ctggagcgca ccaccgccag
    13381 ggtgacgagc agggaaacga cgaaactcag cagcagaaaa ctcattcgat cgcccgaagg
    13441 tggcggcatg acacggtggg cagtgcagcc cggaaacgct ggccggatcg cggcgtagcg
    13501 tcgaggttca gctgcgcaac atcggcgcgg cggcgtcctt ggccgaaagc gaaggtgtgc
    13561 ggccgtccag cggccagggg atcgcgaggt tcgggtcgtc ccatcgaatg cagccttcgg
    13621 agtccgggcg gtaatacgcc gtcgtcttgt acaggaagtc ggcgctgtcg ctggtgacca
    13681 gaaacccgtg cgccagtccc ggcggaatcc agagctgctt gtggttccgt ccgctcagct
    13741 cgacgccgac ccactggccg taagtcgggc tgttctcgcg gatgtcgacc gcgacatcga
    13801 acacgctgcc ctgcgtcacg cggaccagct tgccttgtgc gtgaggatct cgctggtagt
    13861 gcagtcctcg cagcacgccc tgtgtcgacc gagagtggtt gtcctggacg aacgtgacgt
    13921 cgtgcccgac cgccgcctgg aaggtctgca ggttgaagct ttcgaggaag aagccgcgct
    13981 cgtcgccaaa caccctgggt tccaggatca gcacgccgtc cagcgctgtg ggggtcacct
    14041 tcatcagaac acctggttgt tgacgatgcg catcaggtac tgaccgtagc cgttcttcag
    14101 catcggctgg gcgagggctt caagctgggc cgcatcgatc catcccgagc ggaaggcgat
    14161 ctcctcggga caggccacct tcaggccttg gcgtttctcg agggtggcta tgaactggcc
    14221 tgcctcgagc aggctgtcat gcgtgccggt atccagccac gcgtagccgc gccccatgat
    14281 ctcgacgttc agctgccctt gcgcgaggta gcgcgcgttg acatcggtga tctctagctc
    14341 gccgcgcgcc gaagggcgga tgctggcagc gatgtcacac acctgggcat cgtagaaata
    14401 cagtccggtg acggcgtaat tgctctgcgg gaccttcggc ttttcctcga tgctgagcgc
    14461 gcgcttgctc ttgtcgaagg cgaccacgcc gtagcgttcc gggtcctgca cgtggtacgc
    14521 gaacaccgag gctccctcgg tgcgggcatg cgccgcttgc agcaggccct gcagatcgtg
    14581 gccgtggtag atgttgtcgc cgagcaccag cgcactcgga tggccatcga cgaaattccg
    14641 gcccagcacg aaggcctgcg ccagcccgtc cgggctgggc tgcacgcaat agctgatgtt
    14701 cacgccccag cggtgaccgt cgccgagcag cgactcgaag cgcggcgtgt cctgcggagt
    14761 gctgatcaac aggatgtccc ggatgcctgc cagcatcagt gtgctgagtg ggtagtacac
    14821 catcggcttg tcgtagaccg gcagcaactg cttgctgatc gcgagcgtgg ccgggtgcag
    14881 ccggctgcct gagccgccgg ccagcaggat gcctttgcgt tgtgtggtgg tcatgcggta
    14941 ttcgatccgt cggggttcag ccgagcactt cggccagcat tcggtcgacc ccggtctgcc
    15001 agagtggcag gttcaggtcg aaggcttgct ggaacttgcg cgtgtccagc cgcgaattgc
    15061 ccggccgtcg ggctggggtc ggaaaggcgc tggtgggtac cggttcaatg gcttcacgct
    15121 cgacccggat cggacggccc aggcagcgcg cctgctcgat cacgtgaacc gcgtacgcgt
    15181 gccagctggt gaggcccgat gccgcaacgt ggtacgtgcc acagacctgc ggttgccggc
    15241 cggcctgacg caacgcatgg gcggtgacgt cagccagcag gtcggcgcca gtgggtgctc
    15301 cgatctggtc gtcgatgact ttcagcgctg cgcgctccgc agccagcttc agcatggtct
    15361 tggcgaagtt gctgccacgt gcggcgtaca cccagctcgt gcgcaggatc agatgcgggc
    15421 agccgctgcg gcggatggct tcctcgccct cgagcttggt ggtgccgtag acgccgagcg
    15481 gtccggtcgg cgcgtcctcc acccacggtg tgcttccgct gccgtcgaac acatagtcgg
    15541 tgctgtagtg gacgaggctg gcgccgatca cggcggcctc gcgggccagc acgccgggag
    15601 cgatggcgtt gaccgttcgc gccagttcgg gttcgctttc ggcgcggtcg acggcagtgt
    15661 aggcggcggc attgacgatc caccgtggtg ccaccgcgcg cacggtcgcg gccagtgcat
    15721 cgggatcgga aaagtctgcg gcgaactccg tgttcctcga accgagtgcg atcacctgac
    15781 ccagaggcgc gagcgcgcgc tgcaattccc agccgacctg cccgtccttg ccgaacagca
    15841 ggatcttcat ccggcggtgc gcgcggcgta gttggtgtcg acccacgcgc ggtacgcgcc
    15901 gctttgcacc cgggagaccc agtcggcatg gtccaggtac cagcgcaccg tcttgcggat
    15961 gccggtgtcg aaggtctcgg ccggtttcca cccgagctcg ctctcgagct tgcgggcatc
    16021 gatggcgtag cgacggtcgt ggccgggccg gtcggtcacg tgcgtgatca ggcgaccgtg
    16081 cggcccggcc ggatccgggc gcatctcgtc cagcaaagcg cagacggtgt gcacgatgtc
    16141 gatgttgggc atctcgttcc agccgccgat gttgtaggtc tcgccgatcc gaccccttgc
    16201 cagcacggcc cggatcgctg cgcagtggtc cttcacatag agccagtcgc gcacctgacg
    16261 cccatcgcca tagaccggca gaggcttgcc ggcgagcgcg ttgacgatca tcagcgggat
    16321 cagcttctcc gggaaatgaa acggtccgta gttgttgctg cagttggtcg tcagcaccgg
    16381 caggccgtag gtatggtgcc aggcgcgcac caggtggtcg ctgccggcct tgctggccga
    16441 atacgggctg ttcggttcgt agagatgtgt ctctgtgaat ggtgcgtcag caggcccgag
    16501 cgagccgtag acctcgtcgg tggacacatg atggaatcga aactcgccct gctcggcggg
    16561 cggcagcgtc gaccagaacg cgcgcgctgc ctccagcagc gtgaaggtgc cctgcacatt
    16621 ggtgtggatg aatgcgcccg gcccgtggat gcttcggtcg acatggcttt cggcggcgaa
    16681 gtgcaccact gcgcgcggcc ggtgcgtggt aaataacgcg tcgagcagcg ctcgatcggt
    16741 gatgtcgccg tgcacgaaca cgtggcgcgc gtcgccgtcg agcgagcgca ggctttcccg
    16801 gttgccggcg taggtcagtg cgtcgaggtt cagcaccggc tctctcgact gatccagcca
    16861 gtcgagcaca aagttggcgc cgatgaagcc ggctccgcct gtcacgagga tcatgggtgc
    16921 tcttgtgggg gggggctctg cgcggctgtg cagaggccga tttgtggggt gcgaaggatt
    16981 ctaggggtgc cttccggtgg attcccaacc catggcgcca aggaagataa tccaaggatg
    17041 actgaaacac cccaagaaaa tacctctggc gcccctgcga ccgaagcagt tgcggcgccg
    17101 gagcgcttcg acacgctggc cctcgatgcc aagctgttgc gcgccgtggc cgattccggc
    17161 tacacgcaga tgacgcccat ccaggccaaa gccattccca tcgtgcttga cgggcgcgac
    17221 gtgatgggtg ctgcccagac cggtaccggc aagacggctg cgttcacgct tccgctgctg
    17281 cagaagatgc tcaagcacga gaacagcagc gcttctccgg cacgccaccc ggttcgcgcg
    17341 cttgtcctcg cacccacccg cgagctcgcc gatcaggtcg ccaacaacgt caaggcctac
    17401 gccaagcaca gcaagctgcg ctcgatggtc gtttttggcg gtatcgacat gaagccgcag
    17461 acggcagagc tgaagaccgg tgtcgaggtg ctgatcgcca cgccgggtcg cctgctggac
    17521 catatcgagg ccaagaactg caacctgggg caggtcgagt atgtcgtgct cgacgaagcc
    17581 gacaggatgc tcgacatcgg cttcctgccc gacctgcagc gcatcctgag ctacctgccc
    17641 aagcagcggc agaccctgct gttctcggcg acgttctctc cggagatcaa gcggctggcg
    17701 gagagctacc tgcaggatcc gatcctggtc gaagtcgcgc ggccgaacgc caccgcgacc
    17761 aacgtcgagc agcgcttctt cagcgtatcc aacgacgaca agcgggcggc cgttctcaag
    17821 atcatccgcg agcggacact cacgcaggcc cttgtcttcg tcaattccaa actgggctgt
    17881 gcgcgcctgg cgcgctcgtt cgagcgcgac gggctgcgca ccaatgcgtt gcatggcgac
    17941 aagtcgcagg acgaacggct gaaggcgctc gaagcgttca agcgaggcga ggtcgacgtg
    18001 ctggtgtgta ccgacgtggc ggcccgcggt ctcgacatcg ccgacctgcc tgcagtgttc
    18061 aatttcgatg ttccgttcaa tgccgaggac tatgtccacc gcatcggacg caccgggcgc
    18121 gccggggcct cggggctcgc cgtcacgctg gtttcgcgcg acgacactcg gcagg
//
LOCUS       DAOORV010000071        18171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3750_length_18171_cov_6.073029, whole genome shotgun sequence.
ACCESSION   DAOORV010000071 DAOORV010000000
VERSION     DAOORV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3750_length_18171_cov_6.073029"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..418
                     /gene="smpB"
                     /locus_tag="PKV56_10720"
     CDS             2..418
                     /gene="smpB"
                     /locus_tag="PKV56_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOX68329.1"
                     /translation="MEERYEAGMMLQGWEIKAIRAGQVQLTDGYVVIRDGELFLIGCR
                     INALRSASTHISPEADRTKKLLLHKEEIKRLVGKVEQKGYTLVPLDLHYKGGLVKAEI
                     ALAKGKAEHDKRDTEKKRDWDREKGRLMRHKVSSKA"
     gene            complement(436..1566)
                     /locus_tag="PKV56_10725"
     CDS             complement(436..1566)
                     /locus_tag="PKV56_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOX68330.1"
                     /translation="MSGHASKRLPLIDLLKVIASQLIVLHHLAFYGPMSDHAAALMPG
                     LIHWLADPARMVVQVFLVVGGYLAALSLAPDAHLKMPPRVGSRLFERYLRLALPLAVA
                     LFFAIAASSVARGWMDHHSIPDAPRPMQLFWHLLLAQDLVGEEALSAGIWYVAIDLQL
                     YAALLLWMTLVARCSQKSGALRQLLPWGVAGAVVWSALVVNRDPAWDVGAPYFFAANG
                     LGVLAAWARSSRAARAAFGLAMCGVILGLWLEWRDRLALASAVAVALWCWQMWRSGWA
                     FAAGAVGARAIAVLHHLGQISYSVFLMHFSVCLLVNAAFHAFVPADPWLQAFGVLLAW
                     CASVWAGALFHRLAEAPLMRWITRCRATGWQLARRGNWPTAG"
     gene            complement(1563..2759)
                     /locus_tag="PKV56_10730"
     CDS             complement(1563..2759)
                     /locus_tag="PKV56_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOX68331.1"
                     /translation="MADGKPDGDSVGATALIHHGYAPPAGFVAVQPPVHKASTVIFAD
                     THALHSRQWKDKTGYTYGLHGTPTTFTLEERISTLEGGLQTLLLPSGLAAIALVDMAL
                     LRSGDEVLIPDNAYGPGKDLARGELSRWGISHRFYDAMDPASLAAALGPATRLVWLEA
                     AGSVTMEFPDLPALIRLVRAHGAAVVLDNTWGAGIAFNPFEVEPGLGVDISVQALTKY
                     PSGGADILMGSVTTRDHDLHLRLKGTHMRMGWGVGANDAELLLRSLPTLALRYHEQDA
                     ASRRLADWWHRRAEVVQVLHPALPGSPGHASWRHLCTASAGLFSVVFHERYEPQQVHA
                     FVDALRLFKIGYSWAGPVSLVVPYDLGAMRSQCAWKGTLVRFSVGLEAVDDLIGDCEQ
                     ALAALE"
     gene            complement(2762..4171)
                     /gene="rimO"
                     /locus_tag="PKV56_10735"
     CDS             complement(2762..4171)
                     /gene="rimO"
                     /locus_tag="PKV56_10735"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOX68332.1"
                     /translation="MTSEALTASPTTATPSAPKIGFVSLGCPKALTDSELILTQLRAE
                     GYDTSKTYAGADLVIVNTCGFIDDAVKESLDTIGEALAENGKVIVTGCLGAKAGDGGG
                     NLVRQMHPSVLAVTGPHATHEVMDAVHQHAPRRHDPFTDLVPAPDAIGVKLTPRHYAY
                     LKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLGEAQRLFESGVKELLVISQDTSAYGV
                     DVKYRTGFWDGKPVKTRMLDLCEKLGELAAPHGAWVRLHYVYPYPHVDEILPLMAQGL
                     VLPYLDVPLQHSHPDVLKRMKRPASGERNLERIQRWREVCPEIVIRSTFIAGFPGETE
                     AEFEHLLTFIREAEIDRAGCFAYSPVSGAAANELPGALPAEVREDRRARFMAVAEAVS
                     AAKLARRVGATMQVLVDAAPALGRRGGTGRSYADAPEIDGVVKLLPPEKASKTLKVGE
                     FTRARIVGTQGHDLIAQPV"
     gene            complement(4222..4833)
                     /gene="phaR"
                     /locus_tag="PKV56_10740"
     CDS             complement(4222..4833)
                     /gene="phaR"
                     /locus_tag="PKV56_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HOX68333.1"
                     /translation="MPSTRRSAQQPNAQSAEAKTAGDNEGADTRILKKYPNRRLYDTM
                     ASSYITLADVKKMVLSGQPFVVRDAKTSEDLTRSILLQIILEEESGGVPMFSSQMLAQ
                     IIRFYGHAMQGMMGSYLEKNLQTFTEIQGRLADPALGLYDPAALTPEIWGQFLNGQAP
                     AVQGLMSNYLEQSKSMFVQMQEQMAKQAETLFPGMPGFKPPKR"
     gene            5040..5351
                     /locus_tag="PKV56_10745"
     CDS             5040..5351
                     /locus_tag="PKV56_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HOX68334.1"
                     /translation="MSNTNRRTFMMQVVAGGSALAASRVMAQAAVAETDANAVSLGYK
                     ADATKVDAKKYPAHKPTQMCSNCALFQGKPTDAAGGCPLFAGKQVAAKGWCSAYAPKA
                     A"
     gene            complement(5456..5728)
                     /locus_tag="PKV56_10750"
     CDS             complement(5456..5728)
                     /locus_tag="PKV56_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68335.1"
                     /translation="MVDSDWLAPQRLIAVVICFTLLEGAGLWLYRLRTGKGVPGREFV
                     ANWASGLCLMMALLSAVVGSWWLWVAMWLLASGLAHWSDLLARWQR"
     gene            complement(5728..6960)
                     /locus_tag="PKV56_10755"
     CDS             complement(5728..6960)
                     /locus_tag="PKV56_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HOX68336.1"
                     /translation="MSTVETFDVVIVGGGPAGATAAHDLAQQGRSVLLLDRVGRIKPC
                     GGAIPPRLIKDFGIPDELLVAKARSARMISPKDKRVDIPIEDGFVGMVDRDVFDEWLR
                     QRAADSGAVRRNGTFDKLTRDEDGVSVVHFTEREGGRERRSAKPGVLTEGVPTSVRAR
                     AVIGADGARSKVASQAVPGAEDTEYVFAYHEIVRAPAVKPAGYDGSRCDVIYRGTVSP
                     DFYGWVFPHGDTLSIGTGSADKGFSLRGAVADLRQASGLGDTETLRREGAPLPMKPLK
                     RWDNGRDVVLAGDAAGVVAPASGEGIYYAMVGGRLAAESVDGLLRTGDVKSLRLARKR
                     FMKAHGTVFRVLGIMQWFWYANDKRRESFVKICEDKDVQQLTFESYMNKELARKKPMA
                     HVRIFLKDMAHLMGLARA"
     gene            complement(6966..8441)
                     /locus_tag="PKV56_10760"
     CDS             complement(6966..8441)
                     /locus_tag="PKV56_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HOX68337.1"
                     /translation="MSATFGWFGIARLGLVQACLGAVVVLTTSTLNRVMVVELALPAL
                     LPGVLVALHYLIQVVRPRMGHGSDVGGRRTPWLIGGMGTLSVGGVTAAVAVVWMATQP
                     VAGIALAVVGFSLIGLGVSACGTSLLVMLAKRVPAHRRAAAATIVWMMMIAGFAITAG
                     VAGKLLDPYSPQRLLVVTSGVSLLAFGLTLLALRGLEGQSDESSREAQRADAAGPGFR
                     VALSQVWSEPTARRFTIFVFVSMLAYSSQDLILEPFAGSVFGYTPGESTQLSGVQHAG
                     VLLGMVLVALAGSGVAGRLIPRRTASAENASGAATNRLASLRAWTVGGCIASALALIG
                     LAAAGLTGGAWPFKATVFLLGAANGAFSIAAIGSMMSLASQGRESREGVRMGLWGAAQ
                     AVAFGLGGLVGTGASDLARWWFGEAGPAYSAVFAFEAVLFVASAWLAFHGTRGSAASL
                     PVADPDLSMASHRGATLVSAPRAAGREVQAGEVQVAAIQSR"
     gene            complement(8438..9352)
                     /gene="chlG"
                     /locus_tag="PKV56_10765"
     CDS             complement(8438..9352)
                     /gene="chlG"
                     /locus_tag="PKV56_10765"
                     /EC_number="2.5.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthase ChlG"
                     /protein_id="HOX68338.1"
                     /translation="MTTRAMTPPSSVPSWPVVAELLKPITWFPPMWAFCCGVIASGVS
                     PDGRWFVIVTGILLAGPLVCASSQAVNDWFDRHVDAINEPHRPIPSGRMPGRWGLYIA
                     IAWTLLSLLVATTLGTWGFAAGAAGLLLAWAYSAPPLRLKQNGWWGNAACGLCYEGLA
                     WLTGAAVMAGGAMPDGRSLALAALYSAGAHGIMTLNDFKAIEGDRRMGIASLPVQLGA
                     QRAARVACLSMLAPQFVVVLLLIFWGAPVHAVAVGGLMLLQAILMDHFLDRPIQRALL
                     YSGFGVPLFVAGMMVSAFALRTLGTGAA"
     gene            9468..11093
                     /locus_tag="PKV56_10770"
     CDS             9468..11093
                     /locus_tag="PKV56_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HOX68339.1"
                     /translation="MKPSSFLERGFLAPVDTRSAPPPVASAPTAVVIGSGFGGLAAAI
                     RLQARGYQVTVLEKLDAPGGRAYVHRQDGYTFDAGPTIITVPFMLEELWALCGKKLSD
                     DVDLRPLDPFYRIQFDDGTHFDYSGDLDAMRAEVARFSPSDLAGFDSFMVESDRCYKL
                     GFEELATVAFDTVGDLLAAVPSMMKMRAWQTLYAMTARHLRDPKLRIVFSFHPLLIGG
                     NPFACTCVYSLITALERKFGVHWAMGGTGTLIKGLAGLLEGSGGRLRLNAEVREITCV
                     ATAGAKARATGVTLASGEHLQADIVVSNADTAWTYRHLIRPEFRRHWSDRKIDKGRYS
                     MSLFVWYFGTDRQYPEVPHHMMVLGPRYRELLNDIFKEHVLAEDFSLYLHRPTASDPS
                     MAPPGCDAFYVLAPVPHLDSGTDWESKAEPYRQAIADALDRKVMPGFQKHIATSRMTT
                     PQDFHDRLLSFKGAAFGLEPVLLQSAWFRPHNRSEDVDGLFMVGASTHPGAGVPGVLG
                     SAKALESVVPDVSLRGTAAAPSQSIAQVSPSRA"
     gene            11125..12321
                     /locus_tag="PKV56_10775"
     CDS             11125..12321
                     /locus_tag="PKV56_10775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOX68340.1"
                     /translation="MQPHLATLDSRTDTALTQESQDPEASFEVRRRMDLRDCEALMRT
                     GSKSFFAASLVLPGRVRGPATALYAFCRLADDTIDLESHLYGGPMGALQHLQQRLQRI
                     YDGRPSPVPADRALAAVVAKFKLPKALLDALLEGFEWDLQSRRYDTIEQLHDYGARVA
                     GTVGSMMAVIMGARSPQAQARACELGVAMQLTNIARDVGEDARAGRLYLPRDWMREAG
                     IDPEAWLQAPSFTSALGSVVSRLLAEADVLYERAALGISALPRDCRPAIQAARLVYAE
                     IGREVERAGCDSVSRRASVSSQRKLGLMAVAFGAAAVSPARRRSGDDLDPAPLPAIQY
                     LVDAAAIPTPTGDDKPHALRGVRPARFDQRVLWTIGLFERLEERQRASNSFNGAGQQQ
                     PSVFSG"
     gene            complement(12346..13056)
                     /locus_tag="PKV56_10780"
     CDS             complement(12346..13056)
                     /locus_tag="PKV56_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HOX68341.1"
                     /translation="MTERSRASLHRDATRLVIGPSQLHWNGSCLTIDLDEVSVPIPRR
                     VRGQVRVYPKGLCRFVAALDDKGLHRWGPIAPCARVEVDFDSPAASWTGHAYLDSNEG
                     DEPIDRPFVEWDWSRAPLKDGSTGVIYDVRQKEGADRVIAVRFQPSGDFEHFKAPERQ
                     PLPRTAWRIDRNMRTDARHPASVTQTLEDTPFYVRSVLTSGLLGESVVSVHETLNVPR
                     LVSTAVQLMLPWRMPRVR"
     gene            complement(13188..14786)
                     /gene="crtI"
                     /locus_tag="PKV56_10785"
     CDS             complement(13188..14786)
                     /gene="crtI"
                     /locus_tag="PKV56_10785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOX68342.1"
                     /translation="MVDALRAHRVVVVGAGVAGLVSALLLACRGLQVTLIERAETPGG
                     KMRRVVVDGAGVDSGPTVFTMRWVFEQIFEAAGSSLTEELRLQPLGVLARHAWGGDQR
                     LDLHADSARSADAIAQFAGPGEARRFMRFCDQARKVYDTLEGPYIRSQRPTMLSMARD
                     LGPSGLGVLAGLGPFASLWRSLARHFHDARLQQLFGRYATYCGSSPFAAPATLMLVAQ
                     VEMDGVWAVDGGMLAVATTLAALAQKRGATLRYGQACDEVLVRDGRACGVRLADGTCI
                     DADSVVFNGDAAALSVGLLGDRARTAVPEIAASRRSLSAMTWSMHARTSGFPMVRHNV
                     FFNSDYASEFRDIFQHARLPREGTVYVCAQDRDDSGRLPPCEGDPDGSRAERLLCLVN
                     APAHGDSAPLEPSEIEACERTHFALMKRSGLTIDRQAHNTVITTPTGFHRLFPATGGA
                     LYGQASHGWMGVFQRPAAVSRLPGLYLAGGSVHPGPGVPMAAMSGRLAAETLMAHLDS
                     TNRSRRVHISGGMSTRSATTGNMR"
     gene            complement(14779..15444)
                     /locus_tag="PKV56_10790"
     CDS             complement(14779..15444)
                     /locus_tag="PKV56_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spheroidene monooxygenase"
                     /protein_id="HOX68343.1"
                     /translation="MVLVDIAPGSKAWGWTRFVFGRFALRSTPGLRFFKVLGCGYDSG
                     FGLRPSLSRQGVFCVFDGDDTADRFLDSSAPARAYQARSSEYFAVKLHPFSSRGSWSG
                     MSLPVTASAPESGPVAALTRASIRPGLARAFWKQAPLTQRSLVDAPGCLFSAGVGEAP
                     LLRQATFTMWESVALMDAYARSGAHLDAIKASQRNGYFSESMFVRFVPCGARGVWMGR
                     HHG"
     gene            complement(15594..17030)
                     /gene="ppsR"
                     /locus_tag="PKV56_10795"
     CDS             complement(15594..17030)
                     /gene="ppsR"
                     /locus_tag="PKV56_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HOX68344.1"
                     /translation="MTPFSSPTRTLGDLSATVAAKLIATAADVALIVDAEGVICDMAF
                     GSDSLKDLGYERWVGQLWIQTVTKESRPKIEALLREADSVTAPRWRQVNHPSEAGADL
                     PLAYCAVKVDDVSRADSPGHSVVFGRDLRAMASMQQSLVDAQQASLHEHWRLRDAQSR
                     YRHLFQGSSEAVLIIDSVTQKVQEANPAAIQLFADVARKLVGVSFPVGLDESGSEAVQ
                     TMLASLRASGKADDLRVSLTGGNGEVNISGSMFRQEVGSLLVLRLSPATPDGASISKS
                     GGHSMLSKLIQCSPDCVVVTDIDGSIVSANDSFIDLVQVPNEKQVRGESLGRWLGRTG
                     VEMNVLISTLRQRGSVRLFPTTMRSDQGTLSDVEISATMVTEGEQSFLGFTIRDVSQR
                     LANDARAAKELPRSVGQLSELVGRVPMKDIVGETTDLIEQLCIEAALELTRDNRAAAA
                     EMLGLSRQSLYVKLRRYGLSDPVAESEK"
     gene            complement(17102..18034)
                     /locus_tag="PKV56_10800"
     CDS             complement(17102..18034)
                     /locus_tag="PKV56_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOX68345.1"
                     /translation="MTRLTLSAFATEFEEAAADLAAARLWPDGHRLPFNSDSPRWIQA
                     RLERPAAEERMTRLVKTLEAEIIPRLVEAHRCPGAVSDASVAGPHPTGADVDAFVQLL
                     LRREQPPIAAHVSALRDQGVSIEVLYLQLLAPAARQLGCLWTADECDFTDVTLGLGRL
                     QCLLQEFSPEFSTEAQFPANARRVLLLPAPGEQHTFGLSMVGDFFMHAGWEVTSGQGA
                     RRTEAIDMVRREWFDVVGFSVGTEARLDWLTSSIVSVREVSRNKAIGVLVGGPIFTLH
                     PEYAALVGADATSTDGKQAPVAAEKLVASLSKSP"
BASE COUNT         3466 a         6113 c         5739 g         2853 t
ORIGIN      
        1 catcgaagaa cgctacgaag ccggcatgat gctgcagggc tgggagatca aggccatacg
       61 cgcgggccag gtccagttga ccgatggcta cgttgtcatc cgagacggcg agctgttcct
      121 gatcggctgc cgcatcaatg cattgcgcag cgcatcgacc cacatcagcc ctgaggcaga
      181 ccgcaccaag aagctgctgt tgcacaagga ggaaatcaag cggctggtcg gcaaggtcga
      241 gcagaagggc tacacgctgg tgccgctgga cctccactac aagggcggac tggtcaaggc
      301 cgagatcgca ctggccaagg gcaaggccga gcacgacaag cgcgacacgg agaagaagcg
      361 ggactgggat cgggaaaagg gacgcctgat gagacacaag gtctcatcga aggcctgagg
      421 ctttcgcgcc tcgcatcacc ccgcagtcgg ccagtttccg cgccgagcca gctgccagcc
      481 tgtcgcgcga caacgcgtga tccagcgcat caagggagcc tcggccagcc gatggaacag
      541 cgcaccggcc cagacgctgg cacaccaggc cagcaagact ccgaacgctt gcagccaggg
      601 atcggctgga acgaacgcat ggaaggctgc gttgaccagc aggcagaccg aaaagtgcat
      661 caggaacacc gagtagctga tctgcccgag gtggtgcagc acagcgatgg cgcgagcacc
      721 cacggcaccg gcagcgaagg cccagccgct tcgccacatc tgccagcacc acagtgcgac
      781 tgccaccgcg cttgccagag ccagccggtc ccgccactcc agccacaggc ccaggatcac
      841 gccgcacatg gccaggccga atgcggcacg cgccgcccgg ctgctgcgtg cccaggcagc
      901 caacacacca agcccgttgg cggcaaagaa atagggcgcg ccgacatccc acgccggatc
      961 acggttgacg acaagcgccg accagaccac cgcgccagcg acgccccacg gaagcagctg
     1021 ccgaagcgca ccgctcttct gactgcagcg agcgaccagg gtcatccaca gcaacagcgc
     1081 ggcatacagc tgcaagtcga tcgcgacgta ccagatgccc gccgacagcg cctcctcacc
     1141 caccagatcc tgggccagca gcaggtgcca gaacagctgc atcggacgtg gtgcatcggg
     1201 tatcgagtgg tgatccatcc agccccgtgc gaccgaagac gcggcgatcg cgaagaacaa
     1261 ggccaccgcc agcggcagtg ccagacgcag gtaacgctcg aacaggcgcg aaccgacccg
     1321 tggcggcatc ttcaggtgcg cgtcgggcgc caggctgagg gccgccaggt agccgccaac
     1381 gaccaggaac acctgcacga ccatgcgcgc aggatcggcc agccagtgga tcagccccgg
     1441 catcagcgca gccgcgtggt cggacatcgg cccgtagaac gcgaggtgat ggagcacgat
     1501 caactgcgac gcgatcacct tcagcaggtc gatcagcggc aggcgtttcg atgcatggcc
     1561 gctcattcca gtgcggccag tgcctgttcg cagtcgccga tcagatcgtc gactgcttcg
     1621 aggcccaccg aaaaccgcac caacgtgcct ttccatgcgc actgagagcg catggcgccc
     1681 aggtcgtagg gcaccaccag gctgacgggg ccggcccacg aataaccaat cttgaacagg
     1741 cgcagcgcat cgacgaacgc atgcacctgc tgcggttcat accgttcgtg gaagacgaca
     1801 gagaacaagc ccgccgacgc ggtgcaaagg tgacgccagg acgcatgccc cggcgacccg
     1861 ggcagcgccg ggtgcagcac ctgcacgacc tccgctcttc gatgccacca gtccgccaac
     1921 cgacgcgagg ccgcatcctg ctcgtgatac cgcaaggcca gtgtcggaag cgaccgcagc
     1981 agcaactcgg catcgttggc acccacaccc cagcccatcc gcatgtgcgt gcccttcagc
     2041 cgcaggtgca ggtcgtgatc gcgcgtggtc accgagccca tcaggatgtc cgcgccaccc
     2101 gaggggtact tggtcagggc ctgaaccgag atatcgacgc ccagacccgg ttcgacctcg
     2161 aaggggttga acgcgatgcc ggcgccccag gtgttgtcca gcacgaccgc agcaccgtgc
     2221 gcgcggacca ggcggatcaa tgccggcagg tccggaaact ccatcgtcac cgaaccggcc
     2281 gcttccagcc acaccagtct ggtggccgga cccaatgcgg cagcgagcga agccggatcc
     2341 atggcgtcgt agaaccgatg cgaaatgccc caccgcgaga gctctccccg ggccagatcc
     2401 ttgcccggtc cataggcgtt gtcggggatc agcacctcgt caccggagcg cagcagcgcc
     2461 atgtccacca gggcgatcgc cgccagcccg ctgggcagca gcagggtctg caagccgcct
     2521 tccagcgtcg atatgcgctc ttccagcgtg aaggtggtgg gcgtgccgtg caggccgtac
     2581 gtgtagcccg tcttgtcttt ccactgtctg gagtgcaacg cgtgcgtgtc ggcgaagatg
     2641 accgtggatg ccttgtgcac cggcggctgc accgccacga atccagccgg cggcgcatag
     2701 ccgtgatgga tcagcgcggt cgcgccgacg gagtcgccgt caggcttgcc gtcggccatg
     2761 gtcagaccgg ctgcgcaatc aggtcgtggc cctgcgtgcc gacgatgcgt gcgcgcgtga
     2821 attcgcccac cttcagcgtc ttcgatgcct tctcgggagg cagcagcttg acgacaccgt
     2881 cgatctccgg tgcatcggcg tagctgcgac cggtgccgcc gcggcggccc agcgcgggcg
     2941 cggcgtcgac cagcacctgc atcgtcgcac cgacccggcg ggcgagcttg gcggcggaga
     3001 ccgcttcagc cactgccatg aagcgagcgc ggcgatcttc acgaacttct gcaggcaacg
     3061 cccccggcag ttcgttggcc gcagcgcccg acactggtga gtaggcaaag cagccggccc
     3121 gatcgatctc ggcttcgcgg atgaaggtca gcaggtgctc gaattcggcc tcggtctctc
     3181 ccgggaaacc ggcgatgaag gtgctgcgga tcacgatctc ggggcagacc tcgcgccagc
     3241 gctggatgcg ctcgaggttg cgctctccgc tcgccgggcg cttcatgcgc ttgagcacat
     3301 cgggatgcga atgctgcagc ggcacgtcga ggtacggtag caccagtccc tgcgccatca
     3361 gcggcaggat ctcgtcgacg tgcggatagg gatagacgta gtgcagtcgc acccaggcgc
     3421 catgcggcgc ggcgagttca cccagcttct cgcacaggtc gagcatgcgg gtcttgaccg
     3481 gcttgccgtc ccagaagccg gtgcggtact tgacatcgac accgtaggcg ctggtgtcct
     3541 ggctgatcac cagcagttcc ttgacaccag attcgaacaa ccgctgcgcc tcgcccagca
     3601 cgtcaccgat cggacgcgaa accagatcgc cccgcatgct ggggatgatg cagaaggtgc
     3661 agcggtggtt gcagccttcg ctgatcttca ggtaggcgta gtggcgaggc gtcagcttga
     3721 cgccgatggc atcgggggcc ggaaccaggt ctgtgaacgg gtcatgccgc cttggtgcgt
     3781 gctggtggac cgcgtccatc acctcgtgag tcgcatgggg accggtgacg gccagcacgc
     3841 tcgggtgcat ctgccgaacc aggttgccac cgccgtcgcc ggccttggca cccaggcagc
     3901 ctgtgacgat caccttgccg ttctcggcca gcgcttcgcc gatggtgtcg aggctttcct
     3961 tcacggcatc gtcgatgaag ccgcaggtgt tgacgatcac caggtcggcc cccgcatagg
     4021 tcttggaggt gtcgtagccc tcggcgcgca gttgggtgag gatcagttcc gaatcagtca
     4081 gcgccttcgg gcagccgagc gacacgaagc cgatcttggg tgcacttggg gtggcagtgg
     4141 tcggagacgc ggtgagggct tctgaggtca tccgcgcatt ttcgcctcaa aggcccgcgc
     4201 gggcctggag ggtgatgcgc ctcaacgctt cggcggcttg aaaccaggca tgccgggaaa
     4261 aagcgtctcc gcctgcttgg ccatctgttc ctgcatctgc acgaacatgg acttcgactg
     4321 ttcgaggtag ttgctcatca ggccctggac ggccggtgcc tgaccattca ggaactggcc
     4381 ccagatctcc ggtgtcagcg cggccgggtc gtagagaccg agcgctggat cggccagccg
     4441 cccctggatc tcggtgaagg tctgcaggtt cttctcgagg taggagccca tcatcccctg
     4501 catcgcgtgg ccgtagaacc ggatgatctg cgccagcatc tggctcgaga acatcggcac
     4561 accgccactc tcctcctcga gaatgatttg aagcaggatg ctgcgcgtca ggtcttcgga
     4621 tgtcttggca tcacggacca cgaacggctg gccggacaga accatcttct tgacatcggc
     4681 aagcgtgatg tagctgcttg ccatggtgtc gtagagccga cggttggggt acttcttcag
     4741 gatccgggtg tctgcgcctt cgttgtcgcc tgccgtcttt gcctccgcgc tctgagcatt
     4801 cggctgctgg gcagagcgtc gggtcgatgg catcggaaat tccctgaagt tcaaattgtg
     4861 cgtgtgcagc aaaaattgta ggagcgcgat cgagacgact cagccggatt aacccgcgct
     4921 gacccgctct tcgaacgcga tggtttgcca aatcactctg gattcacagt cgtgcaagaa
     4981 tttgtcatcc gaagtcatcc tcggacgtta gctgagttcc caacaacgga gatcccctca
     5041 tgagcaacac caatcgtcgt accttcatga tgcaggtcgt ggctggcggc agcgccctcg
     5101 ccgcctcccg cgtgatggcc caggcagctg tcgccgaaac cgacgccaat gctgtctcgc
     5161 ttggctacaa ggccgacgcg accaaggttg acgccaagaa gtatccggcc cacaagccca
     5221 cccagatgtg cagcaactgc gccctgttcc agggcaagcc gaccgatgca gcaggtggct
     5281 gcccgctgtt cgctggcaag caggtcgcgg ccaagggctg gtgcagcgcc tacgcgccga
     5341 aggcggcctg atagagcaga tcgaaagatc ttgagggtcc gcaaagccgg gccccctgca
     5401 agaaaagccg ggtcgctcac gcgacccggc ttttttgtcg atcggcttgc gaagctcagc
     5461 gctgccagcg tgccaggagg tcgctccagt gggccaggcc ggatgcaagc aaccacatcg
     5521 cgacccaaag ccaccacgaa ccgaccacgg cgctgaggag cgccatcatc agacacagtc
     5581 cggaagccca gttggcgacg aattcacggc ccgggacacc cttgcccgtg cgcaagcggt
     5641 aaagccacaa ccccgccccc tcgagcagcg tgaaacagat gacgacggca atgagccgct
     5701 gcggcgcaag ccagtcgctg tcgaccatca ggcgcgggcc aggcccatca ggtgcgccat
     5761 gtccttcagg aagatgcgca catgggccat cggcttcttg cgagcgagtt ccttgttcat
     5821 gtaggactcg aacgtgagtt gctgaacgtc cttgtcctca cagatcttga caaagctctc
     5881 gcgccgcttg tcgttcgcat accagaacca ctgcatgatc ccgagcaccc ggaacaccgt
     5941 gccgtgcgcc ttcatgaagc gcttgcgtgc gagtcgcagc gacttgacat cgccggtacg
     6001 cagcagcccg tcgacagatt cggcggccag tcgcccgccg accatcgcgt agtagatacc
     6061 ctcgcccgag gctggggcaa cgacaccagc ggcatcgcct gccagcacca catcgcgccc
     6121 gttgtcccag cgcttgagcg gcttcatcgg cagcggcgca ccttcgcgac gcagcgtttc
     6181 ggtatcgccc aggcccgaag cctggcgcaa atcggccacg gcgccgcgca acgagaagcc
     6241 cttgtcggcg ctgccagtgc cgatgctgag cgtgtcgccg tgcggaaaca cccagccata
     6301 gaagtcgggg gacaccgtgc ctcggtagat cacgtcgcag cgcgagccgt cgtacccggc
     6361 tggcttgacg gccggtgccc gcacgatctc gtggtacgcg aacacatact cggtgtcctc
     6421 ggcaccgggc actgcctggc tggcgacctt ggagcgagcg ccgtcggctc cgatgaccgc
     6481 gcgggcacgc acgctggtcg gcacgccttc ggtcaatacg ccaggcttcg cgctgcgacg
     6541 ctcgcggccg ccctcgcgct cagtgaaatg cacgacgctg acgccatctt catcacgggt
     6601 cagtttgtcg aacgtgccat tgcgccgcac ggcaccgctg tccgccgcgc gctggcgcag
     6661 ccactcgtcg aacacgtcgc gatcgaccat gccgacgaaa ccgtcctcga tcgggatgtc
     6721 gacccgcttg tccttcgggg agatcatgcg cgccgagcgt gccttggcca ccagcaactc
     6781 gtcgggaatg ccgaagtcct tgatgaggcg cggtggaatg gccccgccgc agggcttgat
     6841 tcggccgacg cgatcaagca agagaaccga gcggccctgc tgcgcgagat cgtgcgcggc
     6901 agttgccccg gcgggcccgc cgcccacgat cacgacatcg aatgtttcga cagtactcat
     6961 cgtattcacc tcgattggat ggcggcgacc tgcacttcgc cagcttgcac ttctcgcccc
     7021 gccgctcgcg gtgctgagac cagggtggca ccgcgatgcg acgccatgga aaggtccgga
     7081 tccgctactg gcagtgaagc agcactgccg cgggtcccgt gaaacgccag ccaagccgag
     7141 gcaacgaaga gcaccgcctc gaatgcgaag accgccgaat aggcgggccc ggcctcgcca
     7201 aaccaccagc gcgccagatc gcttgcgccc gtgccgacca gtcccccgag cccgaaagcc
     7261 acggcctggg cggcacccca cagtcccatg cgcacgcctt cgcgtgattc acggccctga
     7321 ctcgccaaac tcatcatcga accgatggct gcaatcgaga aggcaccgtt ggcagcaccc
     7381 agcaggaaaa cagtggcctt gaacggccag gccccgccgg tgagtccggc tgccgcgagt
     7441 ccgatcaagg ccaacgccga ggcaatgcaa ccaccgacgg tccaggcgcg cagagaagca
     7501 aggcggtttg tcgcggcccc agacgcgttc tccgcagaag ccgttcggcg cgggatcaga
     7561 cggccggcga cgccactgcc cgccaaggcg accagcacca tgccaagcag gacgcccgcg
     7621 tgctgaacgc cggacaactg tgtcgattca cccggggtgt agccgaacac cgaaccagca
     7681 aagggctcga ggatcagatc ctgggagctg taggccagca tcgagacaaa cacgaatatc
     7741 gtgaagcggc gcgcggtcgg ctctgaccac acctgagaca gcgcgacccg gaatccggga
     7801 cccgccgcgt ctgcacgctg agcttctcgg gaggactcgt ccgactggcc ttccaggcca
     7861 cgcaacgcca gcagcgtcag accaaaagcc agcagggaca cgccactggt gacgacaagc
     7921 aaccgctgcg gtgaatacgg atcgagcaac ttgccggcca cgccggctgt gatcgcgaag
     7981 cccgcaatca tcatcatcca cacgatggtt gcggcggcag ctcggcgatg ggcaggcacg
     8041 cgcttggcca gcatcaccag caaggaagtc ccacaggcgc tgacacccag cccgatcagg
     8101 gagaagccga ccaccgcgag cgcgatcccc gccacgggtt gtgtggccat ccagaccacg
     8161 gcgaccgccg cggtgacacc accgacagac aaggtgccca tgccaccgat caaccagggc
     8221 gtgcggcgcc cgccgacatc ggacccgtgc cccatcctcg gccgcacgac ctggatcaga
     8281 tagtgcagcg ccaccaggac gcccggcaac agtgccggca atgccaactc gaccaccatg
     8341 actcggttca gggtcgacgt cgtcagaacg accaccgcgc cgaggcaagc ctgcaccagg
     8401 ccgagccgcg caatgccgaa ccagccgaac gttgcgctca tgcagcgccc gttcccagcg
     8461 tacgcaacgc gaaggcgctg accatcatgc cggcgacgaa caacggcaca ccgaagccgc
     8521 tgtacagcag ggctcgctgg atgggtcggt ccaggaaatg atccatcagg atggcctgca
     8581 ggagcatcaa cccgccgact gccactgcgt gcaccggtgc gccccagaag atcaacagca
     8641 ggaccaccac aaactgcggg gccagcatgc tcaggcatgc cacccgtgcg gcacgctgcg
     8701 caccgagctg caccggaagc gatgcgatac ccatgcgcct gtcgccttcg atggccttga
     8761 agtcgttgag ggtcatgatc ccgtgcgccc ccgcgctgta gagcgcggcc agcgcgagtg
     8821 aacgcccgtc aggcatcgct ccgccggcca tgaccgcggc accggtcagc caggccagcc
     8881 cctcatagca aagaccgcag gccgcgttgc cccaccagcc gttctgcttg agccgcagcg
     8941 ggggcgcact gtaggcccag gccagcagca gacctgctgc ccctgcggca aatccccagg
     9001 tgcccagcgt cgtggccacc agcagcgaaa gcagcgtcca cgcgatcgcg atgtaaagcc
     9061 cccagcgacc cggcatgcgc cccgagggta tcggcctgtg gggctcgttg atggcatcga
     9121 catggcgatc gaaccagtcg ttgaccgcct ggctgctggc acacaccagc gggccagcga
     9181 gcaggatgcc ggtgacgatc acgaaccacc gaccatcggg cgatacgccc gacgcgatca
     9241 ccccgcagca gaacgcccac atcggcggaa accacgtgat cggcttcagc aattcggcaa
     9301 ctacaggcca ggagggcacc gacgagggag gagtcatggc acgggtggtc atgcggaaga
     9361 ttctgtgatt gacactttga tgtgtcaatc taaacttaca ctcattgcgg gcgcccgggg
     9421 tcatcgagac actggccagc cggcaccatt gaaggacagc gagacagatg aaacccagtt
     9481 ccttccttga gcgtggattt ctggcccccg tggatacgag gtcggcgccg ccacctgtcg
     9541 cgtcggcccc gactgcagtg gtgatcggaa gcggtttcgg tggcctggcc gcggccatcc
     9601 gtctgcaggc acgcggctac caggtgacgg tgctggaaaa gctcgatgca cccggcggtc
     9661 gagcctatgt gcaccggcag gacggctaca cattcgacgc agggccgacc atcatcaccg
     9721 tgcccttcat gctggaggag ttgtgggcgc tgtgcggcaa gaagctgtcg gacgacgtgg
     9781 atctgcgacc gctggaccct ttctaccgga tccagttcga cgacggcacc catttcgact
     9841 acagcggtga cctggatgcg atgcgtgccg aggtcgcacg cttttccccg tccgacctgg
     9901 ccggcttcga ctcgttcatg gtcgagtcgg accgctgcta caagctggga ttcgaggaac
     9961 tggcaacggt cgccttcgac accgtgggcg acctgctcgc cgcggttccg tcgatgatga
    10021 agatgcgcgc ctggcaaacg ctgtacgcga tgacggcccg gcatctgcgc gaccccaagc
    10081 tgcgcatcgt gttcagtttt catccgctgc tcatcggcgg caatccattc gcctgcacct
    10141 gcgtctacag cctgatcacc gcgctcgagc gcaagttcgg tgtccactgg gcgatggggg
    10201 gtaccggcac attgatcaag gggctcgcag gtctgctcga aggcagtggc ggacgactgc
    10261 ggctgaacgc cgaagtgcgc gagatcacct gtgtcgcgac tgcgggcgcg aaggcgcgcg
    10321 ccaccggcgt cacgctggcc agcggagagc acctgcaggc agacatcgtc gtgtccaacg
    10381 cagacaccgc gtggacctat cggcacctga tccgcccgga gtttcgccgc cactggagcg
    10441 atcgcaagat cgacaagggc cgctactcga tgagcctgtt cgtctggtac ttcgggacgg
    10501 atcggcaata ccccgaagtc ccccatcaca tgatggtcct gggcccgcgc taccgcgaat
    10561 tgctgaatga catcttcaag gagcatgtgc tcgccgagga cttcagcctt tacctgcacc
    10621 ggccgacggc atcggatccg tcgatggcac cgccgggttg cgacgccttc tatgttctgg
    10681 cgccagtgcc gcatctcgac agcggaaccg actgggaatc caaggccgaa ccctaccgac
    10741 aggcgatcgc cgacgcgctg gatcgcaagg tgatgccggg ctttcagaag cacatcgcga
    10801 cctcccgcat gacgacgccg caggatttcc acgaccgcct cctgtcgttc aagggagcgg
    10861 ccttcggcct ggagccggta ctgctgcaaa gcgcctggtt ccgcccacac aaccgcagcg
    10921 aagacgtcga cggcctgttc atggtcggcg ccagcaccca ccctggtgcc ggcgtgccgg
    10981 gcgtgctggg ctcggcaaag gcgctcgaat cggtcgtgcc cgacgtttct ctgcgcggca
    11041 cagctgccgc gccatcccag tccattgcgc aggtcagccc atcccgggca tgactcgcag
    11101 cgaaggccgt acccgaggac tctcatgcag ccacacctcg ccacgctcga ttcccgaacc
    11161 gatacagcgt tgactcagga gtcccaagac cccgaagcca gcttcgaggt gcgcaggcgc
    11221 atggacctgc gcgactgtga ggcactgatg cgtaccggct cgaagagctt ctttgccgcc
    11281 tcgctcgtgc tgcccggccg cgtgcgcggg ccggccaccg cgctttacgc cttctgccgc
    11341 ctggcggacg acaccatcga tctcgaaagc cacttgtacg gcggaccgat gggcgcactg
    11401 cagcacctgc agcaacgact gcagcgaatc tatgacggac ggccttcacc ggtgccggcc
    11461 gatcgcgccc tggccgcagt cgtcgccaag ttcaagctgc ccaaggccct gctcgacgcg
    11521 ctgctcgagg gcttcgaatg ggatctgcaa tcgcgtcgct acgacacgat cgagcaattg
    11581 cacgactacg gtgcccgcgt ggcgggtact gtcggctcca tgatggccgt gatcatgggt
    11641 gcgcgcagcc cacaggcgca ggcgcgtgcc tgcgagctgg gtgtggcgat gcaactgacc
    11701 aacatcgcac gtgacgtggg tgaggacgca cgcgccggtc gcctgtatct gccgcgcgac
    11761 tggatgcgcg aggccggcat tgacccggaa gcctggctgc aggcaccgtc tttcacatcc
    11821 gcactcggct cggtggtgtc gcgcctgctg gccgaagccg atgtgctgta cgagcgcgcc
    11881 gcactcggca tctctgccct gccgcgcgat tgccggccag ccatccaggc ggcgcggctg
    11941 gtctacgcgg aaatcggtcg ggaggtcgag cgcgctggat gcgactcggt atcgcgccgg
    12001 gcgagcgtct cgtcgcaacg caagctcgga ctgatggccg tggccttcgg agctgcggcg
    12061 gtgtcacccg cacgccgcag gtccggcgac gacctcgatc cggcacccct gccagcgatc
    12121 cagtatctgg tcgacgcggc ggcgattccc acacccaccg gcgacgacaa gccccatgcc
    12181 ttgcgcgggg tccgcccggc ccgattcgac caacgcgtgc tttggacgat cggacttttc
    12241 gagcgcctcg aagaacgcca gcgcgccagc aactccttca acggcgccgg ccagcagcaa
    12301 ccttcggtat tcagcggctg acgctccggc gggtttcgcc cgctgtcagc gcacccgcgg
    12361 catccgccac gggagcatca gttgcactgc cgtggaaacc aggcggggca cgttcagcgt
    12421 ctcgtgcacc gagaccaccg attcgcccag cagcccggaa gtcagcaccg accggacgta
    12481 gaacggcgtg tcttccaacg tttgcgtgac gctcgcggga tgcctcgcat cggtgcgcat
    12541 gttgcggtcg atgcgccaag cggtgcgcgg cagcggttga cgctccggcg ccttgaaatg
    12601 ctcgaagtcg ccagatggct ggaatcgcac ggcgatcacc cggtcggcac cctccttctg
    12661 gcgcacgtcg tagatcacgc cggtgctgcc gtccttcaat ggcgcacgcg accagtccca
    12721 ttcgacgaac ggccggtcga tcggctcgtc gccctcgttc gaatcgaggt aggcatggcc
    12781 ggtccagctc gcagcagggg aatcgaagtc gacctcgacg cgagcgcatg gcgcgatcgg
    12841 gccccaacgg tgcaatccct tgtcgtcgag cgcggcgaca aagcggcaca ggcctttcgg
    12901 gtagacgcga acctgaccgc gcacccgcct gggaatcggc acgctgacct catcgaggtc
    12961 gatggtcagg caactgccgt tccagtgcag ctgactcggt ccgatgacga gtcgggtggc
    13021 gtcacggtgg aggctggcac ggctgcgctc ggtcatggac cagcgcttgc cagccttgcc
    13081 gtagagcgcc acattgatcg aacagtggtt ctcgggatcg gtggctccgc cgcgtcgcgc
    13141 ccaggcgtag tagggcgaaa agacgcttcc aacgaaggcg atgatgctca gcgcatgttg
    13201 cccgtcgtcg ctgatcgcgt cgacatacca ccagagatat gcacccggcg cgaccggttg
    13261 gtcgaatcga ggtgcgccat cagcgtctcg gccgccagcc gcccggacat cgcggccatc
    13321 ggcacgcccg gtcccggatg cacgctgccc cctgccaggt aaagccccgg caggcggctc
    13381 accgctgccg gtcgctggaa cacccccatc cacccgtgag acgcctggcc gtagagcgcc
    13441 ccgcccgtcg ctgggaaaag ccgatgaaac ccggtcggcg tcgtgatcac cgtgttgtga
    13501 gcctgccggt cgatggtcag gccgctgcgc ttcatcaacg cgaagtgggt ccgctcgcat
    13561 gcttctatct ccgagggttc aagaggtgcc gaatcgccgt gtgcaggcgc attgaccagg
    13621 cagagcagtc gctctgcccg gctgccgtcc ggatcgcctt cacaaggagg cagacggccg
    13681 ctgtcgtctc gatcctgagc gcagacgtag accgtgccct cgcgtggcag ccgggcgtgc
    13741 tggaagatgt ccctgaactc cgatgcgtag tcggagttga agaacacgtt gtggcgcacc
    13801 atcggaaaac cggaggtgcg cgcgtgcatc gaccacgtca ttgcagacag cgagcgcctt
    13861 gatgcagcga tttcgggcac cgctgtccga gcccgatctc cgagcaggcc cacggacagc
    13921 gccgctgcat caccgttgaa gacgaccgaa tcggcgtcga tgcaggtgcc gtctgcgagc
    13981 cgcacgccgc aggcgcgtcc gtcgcgcacc agcacctcat cgcaggcctg cccgtaccgc
    14041 agcgtggcac cgcgcttttg cgccagtgca gccagcgtgg tcgcgaccgc cagcatgccg
    14101 ccatcgaccg cccacacgcc atccatctcg acctgagcga ccagcatcag tgtcgccggc
    14161 gccgcgaacg gcgacgaacc gcagtaggtg gcatagcgac cgaacaactg ctgaaggcgc
    14221 gcgtcgtgga aatgcctggc aagcgaacgc cagagcgagg cgaatggccc cagcccggcc
    14281 agcacaccga ggccgctcgg ccccaggtca cgcgccatgc tgagcatggt gggccgctgc
    14341 gatcggatgt atgggccttc gagcgtgtca tagaccttgc gtgcctgatc gcagaaccgc
    14401 atgaagcgcc gcgcctctcc cggtcccgcg aactgcgcga tcgcatcagc cgaccgggca
    14461 gagtcggcat gaaggtcgag ccgctgatct ccaccccagg cgtgccgcgc aagcacgccg
    14521 agcggctgca aacgcaactc ttcggtcaac gatgaaccgg ctgcctcgaa tatctgctcg
    14581 aagacccaac gcatcgtgaa cacggtcggg ccggaatcta cgccggcgcc atcgaccacg
    14641 actcgacgca tcttgccgcc gggcgtctct gcgcgctcga tcagcgtgac ctgcaggcca
    14701 cgacaagcaa gcaggagagc gctgaccagt cctgccacac cagcgccgac caccacaact
    14761 cggtgcgccc gcaacgcgtc aaccatggtg gcgtcccatc cacacgccac gagcaccgca
    14821 tggaacgaag cgcacgaaca tggactcgga aaagtagcca ttgcgctgcg atgccttgat
    14881 ggcgtcgagg tgtgccccgc tgcgcgcgta ggcatccatg agcgcgacgc tttcccacat
    14941 cgtgaaggtg gcttggcgca gcagcggcgc ctcgcccaca ccggcggaaa agaggcagcc
    15001 cggcgcatcg acgagcgaac gctgcgtcag cggcgcctgc ttccagaacg cccgcgccag
    15061 acccggccgg atcgacgcgc gggtcaaggc tgcaacgggt ccggactcag gcgccgaagc
    15121 ggtgactggc agggacatgc ccgaccagct tccccgactg ctgaaggggt gcagcttgac
    15181 agcgaagtat tcgctggagc gcgcttggta ggctcttgcc ggggctgacg aatcgaggaa
    15241 ccggtcggcc gtgtcgtcac catcgaacac gcagaagact ccttgccgcg aaaggctcgg
    15301 gcgcaagccg aagccggagt cgtagccaca gccgagaacc ttgaagaagc gcaggcccgg
    15361 cgtgcttcgc agggcgaacc gtccgaagac aaaccgcgtc cagccccagg ccttcgatcc
    15421 cggagcgata tcgaccagga ccatcactgc gaccggtcca gagggagacg cgaggacggc
    15481 cggcgattca ggcgccgaca ctggaatcat ctggtggact gcaccagttg caaagacgcg
    15541 ggagcccgca gcgccagcgc cttgggcagc ggacagttcc gctcaactgc accctacttt
    15601 tcgctctcgg cgacagggtc gctcaatccg taacgacgca gcttgacgta aagactctgg
    15661 cgcgagaggc cgagcatttc ggcggctgca gcacggttgt cacgcgtcaa ttcaagcgcg
    15721 gcttcaatgc aaagctgctc gatcagatca gtggtttcgc cgacgatgtc cttcatcggg
    15781 acgcgcccga cgagttcgga caactgaccg accgagcgcg gcaattcctt ggccgcacgg
    15841 gcgtcgttag ccagtcgctg gctcacatcg cggatggtga agcccagaaa cgactgctca
    15901 ccttcggtga ccatcgtcgc agagatctcg acgtcgctca gggtgccttg atcgctgcgc
    15961 atggtggttg gaaacaatcg caccgatccg cgttgtcgca gcgtcgatat cagcacgttc
    16021 atttcgacgc cggtgcgacc gagccagcgg ccgagcgatt cgccacggac ctgcttttcg
    16081 ttggggacct gaaccaggtc gatgaaggaa tcgttggcgg aaacaatcga accgtcgatg
    16141 tcggtgacga ccacgcaatc cggcgaacac tggatgagct tcgaaagcat cgaatgccca
    16201 ccgctcttcg agatcgatgc gccgtcgggt gttgctggtg acaaccgcag aaccagcaag
    16261 gacccgacct cctggcgaaa catcgagccg gatatattca cttcaccgtt gccgccggtc
    16321 agactcacgc gcagatcatc cgccttgccc gacgcgcgca gcgaggccag catcgtctgc
    16381 acggcctcgg accccgattc atcgagcccg acgggaaacg agacaccaac cagcttgcga
    16441 gccacgtcgg cgaacagctg gatggccgcc gggttcgcct cctgcacttt ctgggtcacg
    16501 ctgtcgatga tcagcacggc ttcagacgag ccctgaaaca gatgccgata tcgtgactgg
    16561 gcatcgcgca ggcgccaatg ttcatggagt gaggcctgct gcgcatcgac cagactctgc
    16621 tgcatggatg ccatcgcccg caggtcacgt ccgaagacca cggaatggcc gggggagtcg
    16681 gcgcgactga catcgtcgac tttcacggca cagtaagcca gagggagatc tgcgccggcc
    16741 tcagacgggt gattgacctg ccgccagcgt ggcgcagtga ccgagtcagc ttcgcggagc
    16801 agcgcctcga tcttcggacg actctccttg gtgacagtct ggatccagag ctgcccgacc
    16861 caacgctcgt agccgagatc cttcaggcta tcactaccga aggccatgtc gcagatgaca
    16921 ccttcagcgt ccacgatcag cgcgacatct gcagcggtgg caatcagttt cgcagcgact
    16981 gttgcactga ggtcaccaag ggttcgagtc gggctcgaaa atggagtcat atcgaccccc
    17041 cctttgccat gccaccgaat gcaatcatgg atcagttttt tcagtgaaaa cccgaatttt
    17101 atcaggggct cttggagaga ctggcgacga gcttctcggc agcaacaggc gcctgcttgc
    17161 cgtccgtgct ggttgcatcc gcaccgacaa gcgctgcgta ttcgggatgc agcgtgaaaa
    17221 tcgggccccc gacgagcacg ccaatggcct tgttgcggga cacctcccgc accgaaacga
    17281 tggacgaagt cagccaatcg aggcgtgctt cggttcccac ggaaaagccg actacatcga
    17341 accactcgcg ccgaaccatg tcgatggcct cggtgcggcg cgcaccctgg ccgctggtga
    17401 cctcccaacc ggcgtgcatg aagaagtccc cgaccatcga caggccgaag gtgtgttgct
    17461 cgcccggcgc cgggagcagc agcacgcgcc gcgcattggc tgggaactga gcctcggtgg
    17521 aaaactcggg gctgaactct tgtaacaagc actgcagtct gcccagaccg agggtcacgt
    17581 cggtgaagtc gcactcgtcc gcggtccaga gacagccgag ttgcctggcg gccggcgcca
    17641 gaagctggag atagagcacc tcgatggaga ccccctgatc gcgaagtgcg ctgacgtgcg
    17701 ctgcaatggg tggttgttcg cgtcgaagca gcaactggac aaatgcatcg acatcggctc
    17761 cggtgggatg cgggccggcg acagaggcat cagagacagc gcccggacag cggtgagcct
    17821 cgacgagtcg gggaatgatc tccgcttcga gggtcttcac caggcgggtc atccgctctt
    17881 cggcagccgg acgctccagc ctggcctgaa tccacctggg tgaatcggaa ttgaagggca
    17941 gccgatgccc gtccggccat aagcgggcag ccgccaagtc ggcggcggct tcttcgaatt
    18001 cagtcgcaaa cgcgctgaga gtcaatcgag tcatcagtgg tctcctggcg cggcgcccgc
    18061 gctggctgcg cggcacgtct gtcgtgggcg gagtccggga tgcatcgcct gactgagcgc
    18121 tgcttcctgc ggactgacct gccagaaaag ccgtcgagga catcgaagca t
//
LOCUS       DAOORV010000072        18074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3781_length_18074_cov_7.384983, whole genome shotgun sequence.
ACCESSION   DAOORV010000072 DAOORV010000000
VERSION     DAOORV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_18074_cov_7.384983"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKV56_10805"
     CDS             <1..468
                     /locus_tag="PKV56_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOX68346.1"
                     /translation="TAKKAAPAKKAPAKKAAPAKKAAPAKKAPAKKAAPAKKAPAKKA
                     APAKKAAPAKKAPAKKAAPAKKAPAKKAAPAKKAAAKAPAKKAAAKKVVAKKAAKAPA
                     KKAAAKAPAKKAAAKKAPATPAAAPAAAKPAAPAAKTTLSPQAAWPFPTGSKP"
     gene            complement(555..1460)
                     /locus_tag="PKV56_10810"
     CDS             complement(555..1460)
                     /locus_tag="PKV56_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOX68347.1"
                     /translation="MPALICGSLAFDTITTFPGRFAEQILPDQVHILNVSFLVPTLRR
                     EFGGCAGNIAYTLKQLGGEPVVLAALGTDGAEYLQRLKGWNVTTEHIATISDSFTAQA
                     IIITDSSNNQITAFHPGAMQCAQGTAVPAREDIRIAIIAPDGRDAMIEHAAQLAAAGI
                     PFVFDPGQGLPMFDGSDLRRFVAQARWVAVNDYEARMLCDRTGETLESLSTSHLAGVV
                     VTLGAEGCDVWVQGQCTHVPGIPATEVIDPTGCGDAFRAALLYGLERGWPLLRCVELG
                     NRIGALKIASRGGQNHQIDTGVLDL"
     gene            complement(1561..2676)
                     /locus_tag="PKV56_10815"
     CDS             complement(1561..2676)
                     /locus_tag="PKV56_10815"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOX68348.1"
                     /translation="MNLATRCTACGTIFRVVQDQLKVSDGWVRCGRCQAVFNAQQTLF
                     DLDHEAPPAWQPPEATSVAPESQESPEPFADAPDSGSSTESVPEPAPSNFGERHLLAE
                     QDEPSGSLESPDIDHIEIEPQAPAPQWPEGLRNEFDADIGLPADQADLESESPARTEA
                     PISEQASPSAEAHTDARAAAEVAGNEADSESAPSTPDFVRQAEREEWWEQSPVRAALV
                     ALALTAALGLALQVAGHYRQYIAAQWPESTPWLQHYCEFFGCRLEPLRRIDDVSIEST
                     ALTQADSSLQREGASNALRLAVTLRNRGELPIAMPSLDLSLTGADGELVSRRSLSPAD
                     FQVGDPRLVPGADALLSVSFSVTGRRVSGYTVEVFYP"
     gene            complement(2712..3614)
                     /gene="prmA"
                     /locus_tag="PKV56_10820"
     CDS             complement(2712..3614)
                     /gene="prmA"
                     /locus_tag="PKV56_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOX68349.1"
                     /translation="MVELLLLCPQDEAESVSDALMDELGALSVSVEDADANTEEERAL
                     FGEPGMPAPRGGWDRSIVKALFDGADAAQDAADLLLAQDWASAVQLQSITPLVDQDWV
                     RLTQSQFSPVPVTPSFWVVPSWHEAPAAASKVIRLDPGMAFGTGTHPTTRMCLRWIAQ
                     QTPARAAQWLRVLDYGCGSGILAIGAALHGARGVEAVDIDPAAVQATVDNATANHVNV
                     KAGLPDLASGTYALVLANILATPLKLLAPLLCGHVQAHGDLVLAGILERQADELIEAY
                     APWLDLSVSDSEDGWILMTAQRSA"
     gene            complement(3685..5034)
                     /gene="accC"
                     /locus_tag="PKV56_10825"
     CDS             complement(3685..5034)
                     /gene="accC"
                     /locus_tag="PKV56_10825"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOX68350.1"
                     /translation="MFKKILIANRGEIALRIQRACREMGIKSVVVYSEADRDAKYVKL
                     ADEAVCIGPAASAHSYLNMPAIISTAEVTDAEAIHPGYGFLSENADFAERVERSGFTF
                     IGPTPECIRLMGDKVSAKQAMIKSGVPCVPGGEGALGEDPREIVRSARAIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVQMTRTEAGAAFGNPSVYMEKFLENPRHIEIQVLADQ
                     HRNAVWLGDRDCSMQRRHQKIIEEAPAPGIPRRVIERIGERCAAACKKIGYRGAGTFE
                     FLYENGEFFFIEMNTRVQVEHPVTELVTGIDIVQMQIRVAAMEKLPFTQRQIECRGHS
                     IECRINAEDPYKFTPSPGRITTWHPPGGPGVRVDSHAYTNYFVPPNYDSMIGKIIVHG
                     DTREQALARMRIALSETVIEGISTNIPLHRELMVDAKFVEGGTSIHYLEGWMSEHKR"
     gene            complement(5048..5491)
                     /gene="accB"
                     /locus_tag="PKV56_10830"
     CDS             complement(5048..5491)
                     /gene="accB"
                     /locus_tag="PKV56_10830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOX68351.1"
                     /translation="MDLRKLKTLIDLVSESNISELEITEADGKVRIVKADPHAAAPTY
                     FAAAPMPRPAMAEVAQPAVAAEVAPVGHTVKSPMVGTFYRASSPGGKPLVDIGSDVTE
                     GEAICIIEAMKIMNEIEADKSGKVTRILCENGQAVEFGQPLFIVE"
     gene            complement(5591..6028)
                     /gene="aroQ"
                     /locus_tag="PKV56_10835"
     CDS             complement(5591..6028)
                     /gene="aroQ"
                     /locus_tag="PKV56_10835"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOX68352.1"
                     /translation="MQILVLHGPNLNLLGTREPGVYGHRTLDQIDADLTQRAADAGAK
                     LHTFQSNHEGALIDRVQAARSDGTQFVIINPAALTHTSVGLRDALAAVALPFIEVHLS
                     NVHRREPFRHHSYFSDLAVGVICGLGADGYRLALDHVLTQASH"
     gene            complement(6171..6701)
                     /locus_tag="PKV56_10840"
     CDS             complement(6171..6701)
                     /locus_tag="PKV56_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX68353.1"
                     /translation="MNRRGWMMTTAAITAGATGAGVSWHKTQPADSQVVELGPDFWSL
                     RFEQPGGGELALDSLRGGPLLLNFWATWCAPCIKEMPLIDGFHRAQQPNGWRVLGLAI
                     DSPTPVREFLRKRPVGFPIGLAGLSGTDLSRVLGNPAGSLPFTVIVGRAGRVLDRKLG
                     AIRPEDLTRWAQESEA"
     gene            complement(6698..7297)
                     /locus_tag="PKV56_10845"
     CDS             complement(6698..7297)
                     /locus_tag="PKV56_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68354.1"
                     /translation="MSSALTAEIAAAAARLVVEEGMEYGPAKQRAARALGKHGTRSAQ
                     MPDNGSVEDEVRAYLDLFCADTQPAELAALREVAASWMERLAEFRPHLTGAVWRGTAT
                     RLSSVHLQLFCDDTKSAEIEMLNKGIDFDVGSISGPRNLPVDVLSVSSPSSSLGEEVT
                     VFLTVLDHDDLRGGLKPDASGQTQRGDLVALRRLMETER"
     gene            complement(7311..8093)
                     /locus_tag="PKV56_10850"
     CDS             complement(7311..8093)
                     /locus_tag="PKV56_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOX68355.1"
                     /translation="MKRTTSSALRFLALLALCTVALQLVFGLRIALMTFVDPQSTSFQ
                     RSEMWRLLVEQHEVRWSQRWIAGAQISPNLKRAVIASEDAGFTEHSGVEWDALEKAWE
                     RNQRAEARVAKLNERLEKQAAKAPVTSDTRKTLREPKVIGGSTITQQLAKNLFLAGER
                     SLLRKAQEFIVTFMLEAILGKQRILEIYLNNVEWGEGVFGAEAAARHYFHVPAAQLSP
                     TAAARLAVMLPAPKRFEKRPDSPYVVSRAATVVARMGAVELP"
     gene            complement(8090..9034)
                     /gene="aroE"
                     /locus_tag="PKV56_10855"
     CDS             complement(8090..9034)
                     /gene="aroE"
                     /locus_tag="PKV56_10855"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOX68356.1"
                     /translation="MTEIDHYVVAGNPVAHSQSPFIHAMFAQATGQTLRYGRLLCPID
                     AEGGFAREIRAFAASRGGGNGDFDRTSADAEIGPARGCNVTVPFKFEAYQLGVRHTER
                     ALLAQAANVLRFDAPGAGGWLADNTDGAGLVRDIEHNAGLRLAGRRVLLIGAGGAAAG
                     TLGPLIAARPAAVVLCNRTPDKADALLAGHAAWASRNGVALNVAPIQQPGIGFDVVVN
                     ASASSLQASAPPVPPGVLREGTLALDMMYGAAAQPFITWAESHGAIGRDGLGMLVEQA
                     AEAFLVWRGVRPDTTAVLEKLQQRLAAAANGTAVGTGR"
     gene            complement(9031..9888)
                     /locus_tag="PKV56_10860"
     CDS             complement(9031..9888)
                     /locus_tag="PKV56_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOX68357.1"
                     /translation="MLGKLSALQIAMLVSVAVHGVLLSVRFVDPERFNRIFEDTPLEV
                     ILVNSRSSQAPDLAQAIAQATLAGGGESDRGRATSPLLPSPQAELGDAEDESRKQIEQ
                     LQQTQQQLLTQVRRELAAMPAPETQTEESSAQERAQEEHRRRLVQLLAEIEKRVNEEN
                     ARPKKRYISPATREAVYAQYYDQLRRKIEERGTRNFPEQGGRKLYGELTMNVTVDAAG
                     RVVETEIVRPSKLPVLDRQAIAIVQAAAPFGAFTAKMRAKADQLVVTSRFRFTREDGL
                     ETVPLGAAR"
     gene            complement(9912..11963)
                     /locus_tag="PKV56_10865"
     CDS             complement(9912..11963)
                     /locus_tag="PKV56_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOX68358.1"
                     /translation="MYALYEEAGKFHAGRVMSEADSSMQIELDSGKRVKVKAANVLLK
                     FDKPQPAELISEAQTLARDIDLDLAWEFASDQEFGFADLARDYFDARAGLAQQAAALF
                     RLFEAPHYFRRLGKGNFKKAPEETVKAALLGIERKKQLLAQIEAWATELAEGSCPVPV
                     REQLYKILFKPDKNGPEYKAVVEAAKRSQKAPLDLLTGAGAIDSPYQFHWKRFLFEHF
                     PKGTGFPPLDAPAIREPLPMAAVRAFSIDDSQTTEIDDALSVQGLGTGSVVFGVHIAA
                     PGLAISPDSPVDKVARDRLSTVYMPGHKLTMLPDEVVQVYTLMAGRDCPAVSLYVTLN
                     EDTLEVTGSETRLDRVPIAANLRHDQLDGVITEATLTGAAPADYEFAAELAFAHRLAR
                     HLKAAREVVRGKPENFNRPDYNFRLESALEGEPRGDETVSITTRARGSALDLIVAEAM
                     ILANSTWGGWLADHGVPGIYRSQASMAPGIKVRMGTKPAPHAGMGVAQYTWATSPLRR
                     YVDLVNQWQIIACARHGRTAALAAPFKPKDTALFAIISGFDAAYSAYNGFQSAIERYW
                     ALKYLDQEGITELDAAVMKDGLVRADTLPLVFRALGAEALPRNARVRVRLTGCDLLTL
                     DLHASVSSRIDDPTTEADDAPEIEDPADEAGDSAGPLTLAIDLQDAGAEASPVSPADA
                     P"
     gene            12056..12598
                     /locus_tag="PKV56_10870"
     CDS             12056..12598
                     /locus_tag="PKV56_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOX68359.1"
                     /translation="MVKKIPPRAASPLEAAAGLFDNPLAGTIKDSAQQIWLAGLGAFS
                     KAQEEGGRVFDALVQEGVSMQRKTQSVAEEKLGAVSAQVSARMAEVGQKVGEASARAT
                     GQWDRLETIFEERVSKALASLGVPQADEIRQLTARVEELTAQVAKLTAASNRPARKAP
                     ITRAPAKAVKTSLKARPPKT"
     gene            complement(12604..13152)
                     /locus_tag="PKV56_10875"
     CDS             complement(12604..13152)
                     /locus_tag="PKV56_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOX68360.1"
                     /translation="MEAWLQSLIESLATALALPKFGLSTLFIVSFISATLVPLGSEPA
                     VFGLIKLNPHLFWPAIGVATLGNTLGGMLSWWMGLGAGQAYRAMAARKAVTRSAQVTA
                     PANSDPEQTPTHQRAARWLQRYGAKACLLSWLPVVGDPLCAVAGWLRLPFWPCVLYMA
                     IGKFARYLTMTAALLWLFPGQV"
     gene            13250..14290
                     /gene="dusB"
                     /locus_tag="PKV56_10880"
     CDS             13250..14290
                     /gene="dusB"
                     /locus_tag="PKV56_10880"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOX68361.1"
                     /translation="MNIGHIELTNRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RRDLWHSLKTSRRANHEGEVAPIAVQIAGTDPQMMAEAAAYNIDRGAQIIDINMGCPA
                     KKVCNTWAGSALMQDEPLALKIISAVVAVCEPRRVPVTLKMRTGWCQAERNALPIARA
                     AEAAGIAMVTVHGRTREQGYTGHAEYDTVAAVKAELRIPVVANGDIDSPEKAREVLRY
                     TAADALMIGRAAQGRPWIFREIGHFLATGEHLPAPAVTQAKRWLLEHLHDHYSLYGEF
                     AGVRTARKHIGWAVRSLPGGEAFRAEMNQLETCDKQVRALSDWFDELADRHDLWPAAA
                     NAANQPYIRLIA"
     gene            14287..14526
                     /locus_tag="PKV56_10885"
     CDS             14287..14526
                     /locus_tag="PKV56_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX68362.1"
                     /translation="MSKKKHIEDCIREALEGYFTDLHGVEPAAMYDMILEVVERPLLD
                     VVMKHADHNQSRAAEWLGINRNTLRRKLVEHKLIK"
     gene            14540..>15486
                     /gene="purH"
                     /locus_tag="PKV56_10890"
     CDS             14540..>15486
                     /gene="purH"
                     /locus_tag="PKV56_10890"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOX68363.1"
                     /translation="MHALISVSDKTGVLELARGLHALGIRLLSTGGTAKLLADGGLPV
                     TEVADHTGFPEMLDGRVKTLHPKIHGGLLARRDLSEHRAALTAHGIDTIDVLVVNLYP
                     FEATVAKAGCTLEDAIENIDIGGPAMVRSAAKNWEHVAVLTDASQYAGVLAELEAHGA
                     VPRATRFALAVAAFNRISNYDAAISDHLSSLTLTDGEPARSEYPAQSNGRFVKLQDLR
                     YGENPHQSAAFYRDLHPAPGSLVTAVQLQGKELSYNNIADADAAWECVKSFGPDDAPA
                     ACVIVKHANPCGVAVGSSAGEAYAQAFKTDPTSAFGGIIA"
     assembly_gap    15487..15586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15587..16006
                     /gene="purH"
                     /locus_tag="PKV56_10895"
     CDS             <15587..16006
                     /gene="purH"
                     /locus_tag="PKV56_10895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867393.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOX68364.1"
                     /translation="ADNREIVRSELKVVTKRQPTEAQWRDLLFAWKVAKYVKSNAIVY
                     CGGGMTLGVGAGQMSRIDSARIASIKATNAGLTLAGSAVASDAFFPFRDGLDVVCEQG
                     ASSVIQPGGSVRDDEVIAAADERGIAMVLTGVRHFRH"
     gene            16049..16597
                     /gene="ruvC"
                     /locus_tag="PKV56_10900"
     CDS             16049..16597
                     /gene="ruvC"
                     /locus_tag="PKV56_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOX68365.1"
                     /translation="MRILGIDPGLRITGFGVIESEGSVLRYVASGTIKTDAVAIGELP
                     ARLKIIFDGVREVATRYQPTCAAVEIVFVNVNPQSTLLLGQARGAAITGLVTGGLEVS
                     EYTALQMKKAIVGHGHARKEQVQEMVMRLLALPGLPSKDAADALGLAICHAHAGASFA
                     AMAKGATLARRSHAQFKGGRTY"
     gene            complement(16591..>18074)
                     /locus_tag="PKV56_10905"
     CDS             complement(16591..>18074)
                     /locus_tag="PKV56_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX68366.1"
                     /translation="AHAQAGDQMRLKAVLTQVNVAGTRTRSRVEHAIKDLYSTAISRM
                     RLQAGQLARLATDLMDRNLYERANDCRWWALSPALQRLLAAADAPESGTALNGLLDQV
                     NSLYTVYSRLIAFDAQGIIRGVSHADSGAHLVGTPIDERWLAAVRSLRGSQNYAVSPF
                     EATSVHQHGPTYVYLSKVRDAADGNRHLGGLAVVFHAEREFQTMLDDILGERTGFAAF
                     VDESGAVLSATKGAVMEPAAAEPSCGVDVIELGGFHYAYARVRATGYREFKQSDGYSN
                     GVHAIVALRLGAAERRLTVLSDQQVVSMPIRNRARSVDAAIFQIGSMRCALPSSAVME
                     AISQSGLVRTPHSDGLLRGLIEANSAHGPRLVRVMSGRSLLNLSYPARTTDGIVLVLR
                     SPNDPEVPSLGLLVDDVLAVTEVGLDDLQETPAGMQSYAPWMAGMIRLDAASKGTESL
                     LVQWFDADWFTRQGSHRDQAAVAGHAPVSSDEARGPTAVSSRQ"
BASE COUNT         3002 a         5902 c         5970 g         3100 t
ORIGIN      
        1 actgcgaaga aggccgcccc ggccaagaag gccccggcga agaaggctgc cccggcgaag
       61 aaggccgctc cggccaagaa ggcccctgcg aagaaggctg ctcctgcgaa gaaggcccct
      121 gcaaagaagg ccgctcctgc gaagaaggca gctcctgcga agaaggcccc tgcgaagaag
      181 gcagctccag ccaagaaagc ccctgcgaag aaggctgctc ctgcgaagaa ggccgctgcc
      241 aaggctcctg cgaagaaggc ggccgccaag aaggtcgtgg ccaagaaggc tgccaaggct
      301 cctgcgaaga aagccgctgc caaggctcct gcaaagaagg ctgcagcgaa gaaggcgccc
      361 gctaccccag ccgctgctcc ggcggcggcc aagcccgccg cgccggctgc caagacgacc
      421 ctcagccctc aggctgcatg gccgttccct acaggctcca agccataacg cgacggtccg
      481 cgtatgcggg ctttcgtttg caagcaaccc ggcttcggcc gggttttttt gtgcgcccga
      541 gaacgagccg gcgtctacaa gtccagaact ccggtgtcga tctggtggtt ttgtccgcct
      601 cttgacgcga tcttcaacgc gccgatgcgg ttgcccagtt cgacgcagcg aagcagtggc
      661 cagccacgct ccagcccata cagcaatgct gcgcggaaag cgtccccgca gccggtcggg
      721 tcgatgacct cggtggccgg gatgcccggc acgtgggtgc attggccctg cacccacacg
      781 tcgcagcctt ccgcccccag tgtcacgacg acacctgcca gatgcgaggt ggagagcgac
      841 tcgagcgtct cgcctgtgcg gtcgcacagc atgcgggcct catagtcgtt cacggcgacc
      901 cagcgcgcct gcgctacgaa gcgcctcaga tcgctgccgt cgaacatggg cagtccctgc
      961 cccggatcga agacaaacgg gatgccagcg gctgccagtt gcgctgcgtg ttcgatcatt
     1021 gcgtcgcggc catcgggcgc aatgatggcg atgcggatgt cttctcgcgc aggcacagca
     1081 gtgccttgtg cgcactgcat ggccccaggg tggaacgcgg tgatctggtt gttgctgctg
     1141 tcggtgatga tgatcgcctg cgctgtgaag ctgtcggaga tcgtcgcgat gtgttcggtg
     1201 gtgacgttcc accctttgag tcgctgcagg tattccgctc catcagtgcc cagcgcggca
     1261 agcacgaccg gttccccgcc caactgcttc agcgtgtagg cgatgttgcc ggcgcagcct
     1321 ccgaattcgc gccgcagggt cggtacgaga aacgacacat tcaggatgtg cacctgatcg
     1381 gggaggatct gttcggcaaa acgaccggga aaggtggtga tggtgtcgaa cgcaagtgat
     1441 ccgcaaatca acgcaggcat gggggctccg gtgagggggc gggcgtcgtc accgacggcc
     1501 cgacagggtc agatcgatcg cgacccgaaa cgggcctcaa tccttatttg gcgatggatc
     1561 tcaggggtag aagacctcta cggtgtaacc gctgacgcgg cggcctgtga cagagaagct
     1621 cacggaaagc agcgcgtctg cgccaggcac caaccggggg tcgccgacct ggaagtcggc
     1681 gggagacagc gagcggcggg acaccagttc gccgtcagca ccggtgaggc tcaggtcaag
     1741 cgacggcatc gcgatcggca attcgccccg gttgcgcagg gtcacggcca gacgcagcgc
     1801 attcgaggct ccctcgcgct gcagactgga atccgcctgg gtcagcgcgg tgctttcgat
     1861 gctgacgtca tcgatgcgcc gcaacggttc gagccggcaa ccaaaaaatt cgcagtagtg
     1921 ctgcagccag ggagtcgact cgggccattg cgctgcaatg tactgacgat agtgacccgc
     1981 gacctgcagt gccaggccca gcgcagccgt caacgccagc gcgaccaggg ccgcacgcac
     2041 cggcgactgc tcccaccatt cctcgcgttc ggcctgtcgc acgaagtcgg gcgtggacgg
     2101 cgccgattcg ctgtcggcct cgttgccagc gacttccgcc gctgctcttg catctgtatg
     2161 ggcttcggca ctgggcgaag cctgttcgga aatcggcgct tcggtcctgg cgggggactc
     2221 tgactcgagg tcggcctggt ccgctggcag cccgatgtct gcgtcgaact cgttgcgcag
     2281 gccctcgggc cactgggggg cgggagcttg tggttcgatc tcgatgtgat cgatgtcggg
     2341 tgactcgagc gaacctgacg gttcgtcctg ctctgcgagc aaatgccgtt cgccgaaatt
     2401 cgagggtgcc ggttcgggca cgctttcagt cgatgacccg gagtcagggg catcggcgaa
     2461 cggctctggc gactcctgtg actccggtgc aaccgaggtc gcttcaggcg gctgccacgc
     2521 ggggggagcc tcgtggtcga gatcgaacag cgtttgctgt gcgttgaaca ccgcctggca
     2581 gcgcccgcat cgcacccagc cgtccgacac cttgagctgg tcctgaacca cccggaagat
     2641 ggttccgcat gcggtgcaac gggtggccag attcatcatg gaatgatgcc acgcagcagg
     2701 gtgtttccga ctcaggccga ccgctgtgcg gtcatcaaga tccagccgtc ttcgctgtcg
     2761 ctgaccgaca ggtccagcca gggtgcgtag gcttcgatga gttcgtcagc ctgcctctcc
     2821 aggatgccgg ccagcaccag atcgccatgg gcttgcacgt ggccgcacag cagcggtgcc
     2881 agcaatttca gcggcgtcgc caggatgttc gcgagcacga gcgcgtaggt gccgcttgcc
     2941 aggtcgggca ggccggcctt cacgttgacg tggttggccg tggcgttgtc caccgttgcc
     3001 tggaccgctg ccggatcgat gtcgaccgct tcgacgccgc gcgcgccatg cagcgcggcg
     3061 ccgatcgcga ggatgccgga gccgcagcca tagtcgagca cgcgcagcca ctgcgcagcg
     3121 cgggcgggcg tctgctgtgc gatccagcgc aggcacatcc gcgtggtcgg gtgcgtgccg
     3181 gtaccgaacg ccataccggg atcgagccgt atcaccttcg atgctgccgc cggagcctca
     3241 tgccagctcg gcacgaccca gaagctcggc gtcacaggca ccggtgaaaa ctgcgattga
     3301 gtcaggcgaa cccagtcctg atcgaccagt ggtgtgatcg actgcaactg caccgcgctg
     3361 gcccagtcct gcgccagcag caggtcggct gcatcctgtg ccgcatcagc accatcgaac
     3421 agcgccttca cgatcgaacg atcccagccg ccgcgcgggg ccggcatgcc gggctcgccg
     3481 aacaaggcgc gttcctcctc ggtgttcgcg tcggcgtcct cgaccgacac cgagagcgcg
     3541 cccagttcgt ccatcagcgc atcggaaacc gattcagcct catcctgcgg acacagcagc
     3601 agcagttcga ccatccctgt cagccaacga tgtgcagagc ggcccgctcg aggggcagga
     3661 gcgatgcaac gagttggttg tcattcatcg cttgtgctcg ctcatccagc cttccaggta
     3721 atggatgctg gtgccgcctt cgacgaactt cgcgtcgacc atcaactcgc gatgcagcgg
     3781 gatattggtc gaaatgccct cgatgaccgt ctctgacagc gcgatacgca tgcgcgccag
     3841 tgcctgctcg cgggtgtcgc cgtgcacgat gatcttgccg atcatcgagt cgtagttcgg
     3901 cggcacgaaa tagttcgtgt aggcatgcga atcgacccgc accccgggcc cgcccggtgg
     3961 atgccaagtg gtgatgcgcc cgggcgatgg cgtgaacttg taggggtctt cggcgttgat
     4021 gcggcactcg atcgaatgcc cgcggcactc gatctggcgc tgggtgaacg gcagcttttc
     4081 catcgccgcg acgcggatct gcatctgcac gatgtcgatg ccggtcacca gttcggtcac
     4141 cgggtgctcg acctgcacgc gcgtgttcat ctcgatgaag aagaactcgc cgttctcgta
     4201 gaggaactcg aacgtgcctg ccccgcggta gccgatcttc ttgcacgcgg cggcgcagcg
     4261 ttcgccgatg cgttcgatca cgcggcgcgg aatgcccggt gcgggggctt cctcgatgat
     4321 cttctggtgg cgccgctgca tcgagcagtc acggtcgccc agccacaccg cattgcggtg
     4381 ctggtccgcc agtacctgga tctcgatgtg gcgggggttc tcgaggaatt tctccatgta
     4441 gaccgacggg ttgccgaagg ctgcacctgc ctcggtgcgt gtcatctgaa ccgcattcag
     4501 cagggcggcc tccgtgtgca cgacccgcat gccgcggccc ccgcccccgc cagccgcctt
     4561 gatgattact gggtagccga tggcccgtgc ggagcggacg atctcgcgcg ggtcctcgcc
     4621 cagggctccc tcaccacctg gaacgcaagg aacccccgac ttgatcatgg cctgcttggc
     4681 cgacaccttg tcgcccatca ggcggatgca ttcgggcgtc ggcccgatga aggtgaaccc
     4741 gctgcgctcg acgcgctccg cgaagtcggc gttctcggac aggaagccgt agcctgggtg
     4801 gatcgcttcg gcgtcggtga cctcggcggt cgagatgatc gccggcatgt tgaggtagct
     4861 gtgcgccgat gcagccggcc cgatgcacac cgcctcgtcc gcgagtttca cgtacttggc
     4921 gtcgcggtct gcctcggaat agacgaccac cgacttgatg cccatctcgc ggcatgcgcg
     4981 ctgaatccgc agggcgattt cgcctcgatt ggcgatcagt attttcttga acatcaatgc
     5041 gctcgaatca ctcgacgatg aagagtggct gtccaaactc caccgcctgg ccgttctcgc
     5101 aaaggatgcg cgtgaccttc cccgacttgt cggcctcgat ctcgttcatg atcttcatcg
     5161 cttcgatgat gcagatcgct tcgccttcag tgacatccga gccgatgtcc accagcggtt
     5221 tgccgccagg gctggaggcc cgatagaacg tgccgaccat cggcgacttc accgtgtgac
     5281 cgaccggtgc gacttccgcg gccacggccg gttgtgccac ctcggccatc gcaggccgcg
     5341 gcattggcgc agcggcgaag taggtcggcg cggccgcgtg cgggtcggcc ttgacgatgc
     5401 gcaccttgcc gtcggcttcg gtgatttcca gttcggaaat attcgattcg gacaccagat
     5461 cgatcagtgt cttgagcttg cgtaggtcca tgcgggggct ccaacggagt gggttggggg
     5521 tgtcttgggg aaacctgcgt ctgcattcag gtgccgtggc accgtgcttg cggcgtcgac
     5581 gcaggtcgcg tcagtggctg gcctgtgtca gcacatgatc cagtgccaga cggtagccgt
     5641 cggcaccgag gccgcagatc acgccgacag cgaggtcgga aaagtacgag tggtggcgaa
     5701 atggctcacg ccggtgaaca ttcgacagat gcacttcgat gaacggtagc gccactgccg
     5761 ccagcgcgtc ccgcaggccg acactggtgt gggtcaatgc ggccgggttg atgatgacga
     5821 actgcgttcc atcgctgcga gccgcctgca ccctgtcgat cagggcacct tcgtggttgc
     5881 tttgaaaggt atgcagcttt gctccggcat ctgctgccct ctgggtcaga tcggcatcga
     5941 tctggtccag ggtgcggtgg ccgtaaacgc cgggttcacg ggtgcccaac aggttcagat
     6001 tgggtccgtg gagcacgagg atttgcatga ggagagattg gcgggccacc aaggtgcccg
     6061 atcactgtga tgcagcgggc ggactttacg ctgtttgagg tccgtagtcg tgtgtttgaa
     6121 gaaaaatgac gtcttgatcg accgatcggc gggttttgca agctgatcgg tcaggcttcg
     6181 gactcctggg cccaccgcgt caggtcttcg gggcgtatgg cgcccaattt ccggtcaagg
     6241 acccggccgg cccggcccac gatcaccgtg aaggggagcg atccggccgg gttgcccagc
     6301 acgcggctga ggtcggtacc gctcagcccc gccaatccga tcggaaagcc gaccgggcgc
     6361 ttgcgcagga actcccgtac aggcgtcggg ctgtcgatgg ccaggcccag cacacgccag
     6421 ccgttgggct gctgagcacg atggaatccg tcgatcagcg gcatctcctt gatgcacggg
     6481 gcgcaccagg tggcccagaa attcagcagc agcggtccgc cgcgcaggct gtccagcgcc
     6541 agctctccac cgcccggctg ctcgaatcgc agtgaccaga aatcgggccc gagctcgacg
     6601 acttggctgt cggccggctg cgtcttgtgc cacgacaccc cagcgcctgt ggcacccgcg
     6661 gtgatcgccg ccgtggtcat catccatcct cgcctgttca tctctcggtc tccatcaggc
     6721 gccgcaacgc caccagatcg cctcgttggg tctgtccgct cgcgtcgggc ttcaggccgc
     6781 cgcgcaggtc gtcgtggtcc agcacggtca ggaacaccgt gacttcctcg cccagtgagc
     6841 tgctggggct gctgacactc agcacgtcga ccggcaggtt gcgcggcccc gagatgctcc
     6901 cgacatcgaa atcgatcccc ttgttgagca tttcgatctc ggcagacttc gtatcgtcgc
     6961 agaacagttg caggtgcacg ctgctgagcc gggtcgcggt gccccgccag accgcgccgg
     7021 tcaggtgcgg gcggaactcg gccagccgct ccatccagct tgctgccact tcgcgcagcg
     7081 ccgcgagctc cgcaggctgg gtgtcggcgc agaacaagtc cagataggca cgaacctcgt
     7141 cctcgacgga cccgttgtcc ggcatctgtg ccgatcgggt gccgtgcttt ccgagggcgc
     7201 gtgcggcacg ctgcttcgcc gggccgtatt ccatgccttc ctcgaccacc agccgggcgg
     7261 cagcagcggc gatctcggcc gtcagggcag atgacatcgt cggcacctct tcaggggagc
     7321 tcgaccgcgc ccatgcgggc aacgaccgtt gccgcccgcg acacgacata gggtgagtcg
     7381 ggacgcttct cgaagcgctt gggcgccggc agcatcaccg ccagccgcgc cgcagctgtc
     7441 ggtgacagct gcgccgccgg aacgtgaaag tagtgccggg ccgccgcctc ggcgccgaag
     7501 acgccttcac cccactcgac gttgttgaga tagatctcga ggatgcgctg cttgcccagg
     7561 atcgcctcga gcatgaacgt cacgatgaac tcctgcgcct tgcggagcag gctgcgctcg
     7621 ccggccagga acaggttctt ggccagttgc tgcgtgatgg tggagccgcc gatcaccttc
     7681 ggctcccgca gggtctttct ggtgtcgctg gtgaccgggg ctttcgcagc ctgtttttcc
     7741 aggcgttcgt tgagtttggc gacacgcgct tccgcccgct ggttgcgctc ccaggctttc
     7801 tcgagcgcgt cccattcgac cccgctgtgc tcggtgaagc cggcgtcctc ggacgcgatg
     7861 acggctcgct tcaggttcgg cgagatctgc gcaccggcga tccagcgctg gctccagcgg
     7921 acttcgtgct gctcgaccag caggcgccac atttccgatc gctggaagct cgtggactgc
     7981 gggtccacga aggtcatcag cgcgatccgc aggccgaaaa ccagctgcag ggccacggtg
     8041 cacagcgcca gcaatgcgag gaaacgcagc gcggacgagg tcgttcgctt catcgaccgg
     8101 tgccgactgc ggtgccattc gccgccgctg ccagccgctg ctgcagcttc tccagcacgg
     8161 ctgtggtgtc gggccgcacg ccgcgccaga caagaaaagc ctcggccgcc tgctcgacca
     8221 gcatgccgag gccgtcgcgg ccgatcgcac cgtgggattc ggcccaggtg atgaaaggtt
     8281 gcgcggccgc accgtacatc atgtccagcg ccagggtacc ttcgcgcagc acacccgggg
     8341 gcaccggcgg cgcgcttgcc tgaagactgc ttgcactggc gttcacgacg acatcgaatc
     8401 cgatgcctgg ctgctgaatc ggagcgacgt tcaacgcaac gccgttcctg cttgcccagg
     8461 ctgcatgtcc tgcgagcagg gcatctgcct tgtccggcgt ccggttgcac agcacgacgg
     8521 cggcgggccg tgcagcgatc agcggcccga gcgtgccggc tgcagcgcca ccggctccga
     8581 tcagcagcac gcggcggcct gccagccgca ggccagcgtt gtgttcaatg tcgcgcacca
     8641 ggccggcgcc gtcggtgttg tcggcgagcc agcctccagc gccgggcgcg tcgaatcgca
     8701 gcacgttggc tgcctgggcc agcagcgccc gctcggtgtg ccgcacgccg agctggtagg
     8761 cctcgaactt gaagggtacg gtcacgttgc agcctcgggc gggaccgatt tcggcatccg
     8821 cgctggtgcg atcgaagtca ccgttgccgc cgccgcgcga agcagcgaag gcgcggatct
     8881 cgcgcgcgaa gccgccctcg gcatcgatcg ggcagagcag ccgtccataa cgcagggtct
     8941 gcccggtggc ctgcgcgaac atcgcgtgga tgaatggcga ctggctgtgc gccaccgggt
     9001 tgccggcgac gacgtaatgg tcgatttcgg tcatcgtgcc gcccccagtg gcactgtttc
     9061 caggccatcc tcgcgagtga aacggaaacg cgacgtcacg accagctggt ctgccttggc
     9121 cctcatcttc gcggtgaacg ctccgaacgg cgcggccgcc tggacgatcg cgatcgcctg
     9181 acgatcgagc actggcaact tggagggacg cacgatctcg gtctccacga cgcggcccgc
     9241 agcatcgacc gtgacgttca tcgtcaactc gccgtacagc ttgcgaccgc cctgttcggg
     9301 gaagttgcgg gtgcctcgct cctcgatctt gcgacgcagc tggtcgtagt actgcgcata
     9361 gaccgcctcg cgcgtggcgg ggctgatgta ccgcttcttc ggccgggcgt tctcctcgtt
     9421 gacgcgcttc tcgatctcgg cgagcaactg cacgagccgc cggcggtgct cttcctgtgc
     9481 gcgctcctgc gccgagcttt cctcggtctg cgtttccggc gccggcatcg ccgcgagctc
     9541 gcgccgcacc tgggtcagca actgctgctg ggtctgctgc aactgctcga tctgcttgcg
     9601 ggactcgtct tccgcgtcgc ccagctcggc ttgcggtgat ggaagcagag gcgaagtcgc
     9661 gcggccgcga tcggattcgc cgccgccagc gagcgtggcc tgcgcgatcg cctgagccag
     9721 gtcaggtgcc tgtgacgagc gcgaattgac gaggatcacc tccagcggcg tgtcttcgaa
     9781 gatccggttg aaacgttcgg gatcgacgaa gcgaaccgac agcagcaccc catgcacggc
     9841 caccgacacg agcatcgcga tctgcagggc cgacagcttg ccgagcatcg tttcagttca
     9901 ccgtctgcag ttcagggtgc atccgccggc gaaacgggcg acgcttcggc gccggcgtcc
     9961 tgcaggtcga tggccagggt cagcggcccg gcactgtcgc cggcttcgtc ggctggatcc
    10021 tcgatttccg gcgcgtcgtc agcttcggtg gtcgggtcgt cgatgcgcga gctcacgctt
    10081 gcatgcaggt cgagcgtgag caggtcgcat ccggtgagac gcacccgcac gcgggcgttg
    10141 cgtggcagcg cctccgcgcc cagcgcgcgg aacaccagcg gcagcgtgtc ggcgcgcacc
    10201 aggccatcct tcatcactgc agcgtcgagt tcggtgatgc cctcctgatc gaggtacttc
    10261 agcgcccagt agcgctcgat tgccgactgg aatccgttgt aggcgctgta agccgcatcg
    10321 aagcccgaga tgatcgcgaa cagcgcggtg tccttcggct tgaacggtgc cgccagtgcc
    10381 gcggtgcggc catggcgggc gcaagcgatg atttgccact ggttgaccag gtcgacatag
    10441 cggcgcagcg gcgaagtggc ccaggtgtac tgagcgacgc ccatgccggc gtgcggcgcc
    10501 ggcttggtgc ccatgcgcac cttgatgccg ggtgccatgc tggcctggct gcggtagatg
    10561 ccgggcacgc cgtgatcggc gagccagcca ccccaggtgc tgttggccag gatcatcgcc
    10621 tcggcgacga tcaggtcgag cgccgacccg cgtgcgcggg tcgtgatgga gacggtttca
    10681 tcgccacggg gctcgccctc cagggcactt tcgaggcgga agttgtagtc ggggcggttg
    10741 aagttctccg gcttgccgcg aaccacctcc cgcgccgcct tcaggtgccg ggccagccgg
    10801 tgcgcaaagg ccagctcggc agcgaattcg tagtcggccg gggctgcgcc ggtcagcgtg
    10861 gcttcggtga tcacgccgtc gagctgatcg tgccgcaggt tggctgcgat cggcacgcgg
    10921 tcgaggcgtg tctcgctgcc ggtgacttcg agcgtgtcct cgttcagcgt cacgtaaagc
    10981 gacaccgccg ggcagtcgcg cccggccatc agtgtgtaga cctgcaccac ctcgtcgggg
    11041 agcatcgtca gcttgtgacc cggcatgtag acggtggaga gccggtcgcg cgcgaccttg
    11101 tctacgggac tgtccggcga gatcgccagt cctggcgctg cgatgtgcac gccgaacacc
    11161 accgagccgg tgcccaggcc ctgaaccgaa agcgcatcgt cgatttcggt ggtctgcgaa
    11221 tcgtcgatcg agaacgcccg caccgcagcc atcggcagcg gctcccggat ggcgggggcg
    11281 tcgagtggcg ggaagccggt gcctttcgga aaatgctcga acaggaagcg cttccagtgg
    11341 aactggtatg gcgagtcgat cgcgccggcg ccggtcagca ggtcgagtgg cgccttctgc
    11401 gagcgcttgg ccgcttccac caccgccttg tattcggggc cgttcttgtc cggcttgaac
    11461 aggatcttgt agagctgctc gcgcaccggg accgggcagc tgccctcggc cagttcggtg
    11521 gcccatgcct cgatctgcgc gagcagctgc ttcttgcgtt cgatgccgag cagcgcagcc
    11581 ttgacggttt cctcgggcgc cttcttgaag ttgcccttgc caagccgacg gaagtagtgc
    11641 ggcgcctcga acagcctgaa aagcgcagcc gcctgctggg cgaggcctgc gcgcgcatcg
    11701 aagtagtcgc gggcgaggtc ggcaaacccg aactcctggt cggatgcgaa ctcccacgcc
    11761 aggtcgagat cgatgtcgcg ggccagtgtc tgggcttcgc tgatgagttc ggcgggttgc
    11821 ggcttgtcga acttgagcag cacgttggcg gccttcacct tgacgcgctt gccggaatcc
    11881 agctcgatct gcatcgagct gtcggcttcg gacatcacgc gtccggcgtg gaactttcca
    11941 gcttcttcat agagggcgta cataggcggc ggattgtcac cgatgcgctt cggccgacac
    12001 gggcctacag tcttgggtgg gccgcccttt gcaccgcccg ccacaggagc aagtcatggt
    12061 caagaaaatc ccgccccgag ccgcctcgcc gctggaggcg gctgccggtc tgttcgacaa
    12121 cccgctggcc gggacgatca aggattcggc ccagcagatc tggctggccg ggctgggtgc
    12181 gttctcgaaa gcccaggagg agggcggccg ggtgttcgac gcgctggtcc aggaaggcgt
    12241 gtcgatgcag cgcaagacgc agtcggtggc cgaggagaag ctgggtgcgg tctccgcgca
    12301 ggtcagtgcg cgcatggccg aggttggtca gaaggtgggg gaggcgtccg ccagggccac
    12361 cggccagtgg gatcggctgg agaccatttt cgaggagcgt gtgtcgaaag cgctcgcgag
    12421 cctgggtgtg ccgcaggcag acgagattcg ccagctgacc gctagagtcg aggaacttac
    12481 tgcccaggtg gcgaaactga ccgccgcttc gaatcggccc gcacgcaagg ccccgatcac
    12541 gcgcgctcca gccaaggcgg tcaagaccag cctgaaggcc cggcctccga agacctgacc
    12601 cgcctagacc tgcccgggga agagccagag cagggcggcc gtcatcgtga ggtagcgggc
    12661 gaacttgccg atcgccatgt aaagcacgca gggccagaac ggcagcctca gccacccagc
    12721 cacggcgcac aggggatcgc cgaccaccgg cagccacgag agcaggcatg ccttggcgcc
    12781 gtaacgctgg agccagcgcg ccgcgcgttg atgcgtgggg gtctgttccg gatcggaatt
    12841 cgccggtgcg gtgacctggg cggatctggt gacggccttt cgtgccgcca tcgcccgata
    12901 ggcctggcct gcgcccagcc ccatccacca ggagagcatg ccgcccagcg tgttgcccag
    12961 ggttgccaca ccgatcgccg gccagaacag gtgcggattc agcttgatga ggccgaacac
    13021 cgccggctcc gaccccagcg gcaccagcgt ggccgatatg aacgaaacga tgaacagcgt
    13081 gctcagtccg aacttgggca gtgccagcgc tgtcgcgagt gactcgatca acgactgcaa
    13141 ccaggcttcc atgcggccga ttatcgagac ctgaaaatct gtcccgtccg tgctggaggc
    13201 gaagctatac tctgcctcct ttttgagcag accggccccg cctccacgca tgaacatcgg
    13261 ccacatcgag ttgacaaacc gcctgtttgt cgcgccgatg gcgggggtca ccgaccggcc
    13321 cttccgccag ctgtgcaagc gcctgggtgc gggctacgcg gtcagcgaga tggtcacctc
    13381 gcgcagggat ctgtggcaca gcctgaagac gagccgccgt gccaaccacg agggcgaggt
    13441 cgcgccgatc gcggtgcaga tcgccggaac cgatccgcag atgatggccg aggccgcggc
    13501 ctacaacatc gatcgcggcg cgcagatcat cgacatcaac atgggctgtc cggccaagaa
    13561 ggtgtgcaac acctgggcgg gctccgccct gatgcaggac gaaccgctcg cgctgaagat
    13621 catctccgcc gtggtggccg tctgcgaacc tcgccgggtc ccggtcacgc tgaagatgcg
    13681 caccggctgg tgccaggccg aacgcaacgc gttgccgatc gcgcgcgctg ccgaggctgc
    13741 cggcatcgcg atggtcacgg tgcacgggcg cacgcgcgaa cagggctaca ccggtcatgc
    13801 cgagtacgac accgtagccg ccgtcaaggc cgagctgcgc attcccgtgg tggccaacgg
    13861 tgacatcgac agcccagaga aggcccgcga ggtgctgcgc tacaccgccg cggacgcgtt
    13921 gatgatcggc cgcgcggcgc aaggtcgccc ttggatcttt cgggagatcg ggcacttcct
    13981 ggccaccggc gagcacctgc cggcaccggc ggtgacgcag gccaagcgct ggctgctcga
    14041 acacctgcac gaccattaca gcctgtacgg cgaattcgct ggcgtgcgca ccgcacgcaa
    14101 gcacatcggc tgggcggtga gatcgctgcc gggtggcgag gccttccgtg ccgagatgaa
    14161 ccagctcgag acctgtgaca agcaggtgcg ggcactgagc gactggttcg acgaactcgc
    14221 cgaccgtcac gacctctggc ctgctgcggc gaatgcggcc aaccagccct acatccgttt
    14281 gatcgcatga gcaagaagaa gcacatcgaa gactgcatcc gcgaagcgct ggagggttac
    14341 ttcaccgacc tccacggcgt cgagccggct gccatgtacg acatgatcct ggaggtggtc
    14401 gagcggccgt tgctcgatgt cgtgatgaag cacgccgacc acaaccagag tcgcgccgcc
    14461 gaatggctgg gcatcaaccg caacacgctg cgccgcaagc tggtcgagca caagttaatc
    14521 aaatgatccg aaacatccca tgcacgcact gatttccgtt tccgacaaga ccggtgttct
    14581 cgaactcgcc cgcggcctgc acgcgctggg catccggctc ctgtccaccg gcggcaccgc
    14641 gaagctgctc gccgacggtg gcctgccagt caccgaggtc gccgaccaca ccggctttcc
    14701 cgagatgctc gacggccgcg tcaagacgct gcacccgaag atccacggcg ggctgctggc
    14761 gcggcgcgat ctgagcgagc accgtgcggc actgaccgcg cacggcatcg acaccatcga
    14821 cgtgctcgtg gtcaacctgt atccgttcga ggcgaccgtc gccaaagccg gctgcacgct
    14881 ggaggatgcg atcgagaaca tcgacatcgg tggcccggcg atggtgcgca gcgcggccaa
    14941 gaactgggag catgtcgcgg tgctgaccga tgcatcgcag tacgccggtg tgctggctga
    15001 actggaagcc cacggcgcgg tgccgcgcgc cacccggttc gcgttggccg ttgcggcctt
    15061 caaccgcatc tcgaactacg acgcggcaat cagcgatcac ctgtcgtcac tcaccctgac
    15121 tgacggcgaa ccggctcgca gcgagtatcc ggcccagagc aacggccgct tcgtcaagct
    15181 gcaggacctg cgttacggcg agaacccgca ccagagcgcc gcgttctacc gcgacctgca
    15241 tccggcgcca ggctcgctcg tgacggcggt gcagttgcag ggcaaggaac tgtcgtacaa
    15301 caacatcgcg gatgccgatg cggcttggga gtgcgtcaag tcgttcggac ctgacgatgc
    15361 gcccgccgcg tgcgtgatcg tcaagcatgc caacccctgc ggcgtcgcgg tcggcagcag
    15421 cgccggcgag gcctatgccc aggccttcaa gaccgacccg acctcggcgt tcggcggcat
    15481 catcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg acaaccgcga
    15601 gatcgtgcgc agcgaactga aggtcgtcac gaagcggcag ccgaccgagg cgcagtggcg
    15661 cgatctgctg ttcgcctgga aggtcgcgaa atacgtcaag agcaacgcga tcgtctactg
    15721 cggcggcggc atgacgctgg gcgtgggcgc aggtcagatg agccgcatcg attccgcgcg
    15781 catcgcttcg atcaaggcga ccaacgccgg cctgacgctg gcgggatcgg cggtcgccag
    15841 cgatgctttc ttcccgttcc gcgatggact cgatgtggtg tgcgagcagg gggcttcgtc
    15901 cgtgattcag ccgggcgggt cggttcgtga cgacgaagtg attgctgccg cggacgagcg
    15961 cggcatcgcg atggttctga cgggcgttcg gcatttccgg cactgacacg gtgcttgttc
    16021 gcttaggccg tgccagggac atgtcgccat gcgcatccta ggcatcgacc ccggcctgcg
    16081 catcacgggc ttcggcgtca tcgagtccga aggttccgtg ctgcgctatg tcgccagcgg
    16141 caccatcaag accgacgccg tggcgatcgg tgagttgcct gcgcggctga agatcatctt
    16201 cgacggggtg cgcgaggtcg ccacccgcta ccaaccgacc tgcgctgcgg tggagatcgt
    16261 gttcgtcaac gtcaatccgc agtccacctt gctgctcggg caggcacgcg gagccgccat
    16321 caccggtctg gtgaccggtg gcctggaggt gtccgagtac accgccctgc agatgaagaa
    16381 ggccatcgtc ggccacggcc acgcacgcaa ggaacaggtg caggagatgg tgatgcggct
    16441 gcttgcgctg cccgggctgc ccagcaagga cgcagccgat gcgctcggtc tcgcgatctg
    16501 ccacgcccat gccggcgcct cctttgcggc gatggccaag ggcgcgacgc tggcccgacg
    16561 ctcgcatgcc cagttcaagg gcggacgcac ctactgacgg gagctgaccg cggtgggccc
    16621 tcgcgcttca tccgatgaga ccggcgcgtg gcctgcaacc gcggcttgat cgcggtgcga
    16681 gccctgccgc gtgaaccagt ccgcatcgaa ccactgaacc agcagggatt cggtgccctt
    16741 cgatgcagcg tcgaggcgaa tcatcccggc catccagggc gcgtatgact gcatgccggc
    16801 gggcgtttcc tgaaggtcat ccagaccgac ttcggtgacc gccagcacat catcgacgag
    16861 cagtccgagg ctcggtactt cgggatcgtt cggcgagcgc agcaccagca cgatgccgtc
    16921 cgtcgttcga gccgggtagg acagattgag aaggctgcgt ccggacatca cgcgaaccag
    16981 tcgagggccg tgggctgaat tggcctcgat caggcccctc aggaggccgt ccgaatgagg
    17041 ggttcgcacc agaccgctct gcgagatcgc ctccatcact gcagatgacg gcagcgcgca
    17101 gcgcatcgac ccaatctgga agatggccgc gtcgaccgac ctggcgcggt tcctgatggg
    17161 cattgagacg acttgctggt ctgagagcac ggtgagacgc cgctccgccg cacccaggcg
    17221 caaggcgacg atggcgtgga cgccgttgct gtacccatcg ctctgcttga actcgcgata
    17281 accggtggcc cgtacgcggg cataggcgta gtggaatccg ccgagctcga tcacgtccac
    17341 gccgcaggat ggctcggctg cggcgggttc catgacggcg cccttggtgg cggacagcac
    17401 cgccccggat tcgtccacga aggctgcgaa cccggtacgc tcaccgagga tgtcgtcgag
    17461 catcgtctgg aactcgcgct ccgcatggaa cacgacggcc agtccgccca ggtgccggtt
    17521 gccgtccgcg gcatcgcgga ccttcgacag gtatacgtag gtggggccgt gctgatgtac
    17581 ggacgtggct tcgaacggcg acaccgcgta gttctgcgag cctcgaagcg atcggaccgc
    17641 agccagccac cgctcatcga tcggcgtgcc caccagatgg gccccgctgt ctgcatggga
    17701 aacgccccgg atgattccct gcgcatcgaa ggcgatcagt cggctgtaga cggtgtacag
    17761 ggaattcacc tgatccagca ggccattcag cgcggtcccg ctctcgggtg catccgcagc
    17821 cgccagcagt cgctggagtg caggtgacag cgcccaccag cggcagtcgt tggctcgttc
    17881 gtagaggttg cggtccatca ggtctgtcgc cagcctggcc aactgcccgg cctgcagtcg
    17941 catgcgactg atggcggtgc tgtacagatc cttgatcgcg tgctcgacac ggctccgcgt
    18001 gcgcgtgcct gccacattca cctgcgtcag caccgccttc aggcgcatct ggtcaccggc
    18061 ctgggcgtgg gcca
//
LOCUS       DAOORV010000073        17945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3820_length_17945_cov_6.455674, whole genome shotgun sequence.
ACCESSION   DAOORV010000073 DAOORV010000000
VERSION     DAOORV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17945
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_17945_cov_6.455674"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKV56_10910"
     CDS             <1..469
                     /locus_tag="PKV56_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX68367.1"
                     /translation="EPCRGSAALAPPPITAFSIPEPFLHMKFLSLKLGGRACALLLVS
                     SAMAGGSPPPDLLEAPAVVAAPPRSTPFDLSDPDRIKAGKQRFHSTCAEFCHGHAPPL
                     FIGRADLPPRYAFDMISNGGGSATPMPPWGSVFTHDEIWELVAYLKYLGTQKL"
     gene            1212..2354
                     /locus_tag="PKV56_10915"
     CDS             1212..2354
                     /locus_tag="PKV56_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX68368.1"
                     /translation="MGHQFFNLKPAAAGRLLVLAGFMASAGLASAQSSVQVFGIIDAG
                     ILTQTNSADSKKTKTRLETSGLRQSVWGLKGTEDLGGGLKAFFNLESHFDTDTGHIHG
                     TGDSNQSGTILFRRQANVGLTGDWGTVILGRQYGPALLAHIGTEPRAFKEQFSNLYAW
                     AYNQLDGFAGPGAAGNTNDVGIFMSNAVQYRNNFGPVTVGVLYAFGEQSKKISRGNTF
                     AIGVNYNGPVVLSGSYQVMKDKASGEDIIEHYGLGAAVPFGDFTFKLSWLRGENSGAD
                     GVETSKVDGISGGVDWKWSPSNSATLAYYDNKDKNQSSNHTKNIIISNDLFLSKRTTF
                     YTQVAIVDAGKGSTGIAAFKTSVVADASYLPNKTTTFLNVGINHTF"
     gene            2488..4242
                     /locus_tag="PKV56_10920"
     CDS             2488..4242
                     /locus_tag="PKV56_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HOX68369.1"
                     /translation="MFHSRRPAALRLHPLVACIGLGLLVTSVCRAEDPEPPLGVNVLE
                     NTFKWRPNYVSDDMLMHADKDANNWLHYGKDYQNTRFSALTQITTENVKKLVPKWNLS
                     FGVNEAQDSQTTVINGRVFVTASQNKVFALDGVTGKMLWKYERSLPGDLGPKLCCDAV
                     NRGVALYKDMVFMATLDTHVVALNSTTGAVVWEKKMGDYKSGEIYTSMPLVAKGMVIV
                     GNSGGDVGGNVGRITALNPANGEVIWETKTRPTSADHPQAKTWANDSWKTGGSSAWLT
                     GTYDAKTDTVLWGVGNPTPDFDPSVRKGDNLYSNSTLALDASTGKIKYHFQYTPNDSW
                     DYDGNNEVIMVDDEKGRKVWVHGDRNGHLYSIDRTNGKCNWVVPIARVNWVTGFGENC
                     RPIVNPDKVPRYDKIVTDIAPILDGGKEWHPAAYSPRTKLVYIPFIDSSMDIQAKKTE
                     WKHGEWFLSSRVLKANPYTGGVKAFNATTGEQIWTRPQSTPATSGLLATAGKLVFSGD
                     AEGWFSALKDDTGEEMWRFNVGSGIHGNPTTFTADGKQYVAIVYGPGGGSLLPLIYDE
                     LMKRNNRGGGLMVFGLSD"
     gene            4301..5089
                     /locus_tag="PKV56_10925"
     CDS             4301..5089
                     /locus_tag="PKV56_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX68370.1"
                     /translation="MKYLRRFAQAAALLLVAGGLRAAEADAPAYLNVCVDDDNPPFSS
                     EARPERGIDVEVAQAIAGQLGRPLKLVWATVPNRGGIGKALRQSLSAGKCDAYLGIPQ
                     DPDLARELAERKLVTSPPYLVLGYVLVAAPGKPVPTAAALRGARRVGAVTATPADLYL
                     YRKQLGRVPYPGSAALIEALKAGEVDLALVWSSSLTGDAGKSVVAATEAVDDADLYTG
                     MTVATRTADTALANELAKAIDALRSNGRIDTIMQRYGLPRVSRP"
     gene            5114..5590
                     /locus_tag="PKV56_10930"
     CDS             5114..5590
                     /locus_tag="PKV56_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX68371.1"
                     /translation="MSQITNLRVRLASALLVSSLSGSTLAFGPAPASTSGAGSVTLSQ
                     ATESAPAPAPVASPDFEKQGEELFGRNCQLCHNSRGKGGKAPQLLRGAWGPGGANSDE
                     FMFNTIKNGRPNTQMGGFNGSLSDAEIRIIVAFLRSESVRAKANDRKTVSTDYEPW"
     gene            5713..6213
                     /locus_tag="PKV56_10935"
     CDS             5713..6213
                     /locus_tag="PKV56_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOX68372.1"
                     /translation="MSKSSAGCLAAASLMLCAAAAQALDRVPVLSLEVAQTMSRTCET
                     LAKVKGWRMNIAVVDAGTNLIAFSRMDQAFLGSTDIAISKAKFSGSFPFSTRYAGELT
                     FGKDGKPAPVPGLADIPGVVTFAGGLPIMVAGVQVGGIGVSGGSPDEDEQCAAAAIDA
                     VKDSLK"
     gene            complement(6296..7303)
                     /locus_tag="PKV56_10940"
     CDS             complement(6296..7303)
                     /locus_tag="PKV56_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOX68373.1"
                     /translation="MRCYTYLKENNADSLTLQEREVPRPGPGQVLVRMKAASLNYRDL
                     IIVWGRYPGMKSEGLVPGSDGAGVVAEVGPGVTRFASGDRVTGCFFETWLSGEMTPKH
                     YENALGGSVPGVLAEYRVFPEEALVATPAHLSDAEAATLPCAALTAWNALYEGRQLVP
                     GETVLVLGTGGVSMFALQFAKASGATVIATSSSDAKLAKAKALGADHLINYKTNPDWE
                     QRVREVTGGRGVDHVVEVGGPGTLQRSIAAARHGGAVHLIGVLTGGQIDPLAILFNTT
                     LLRGVFVGSRDMFESMNRLISARQIKPVIDRVFPFTEARAALAHLEGASHFGKVVISI
                     D"
     gene            7431..8213
                     /locus_tag="PKV56_10945"
     CDS             7431..8213
                     /locus_tag="PKV56_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX68374.1"
                     /translation="MKKKKQTPELAPATISEADGVRYLHLGTPWVQGAMRIRKPLAID
                     LEYVQRMMVWMLLRPSSDWPECHAVQLGLGAGAITRYCHGVLRMRCTAVELNDTVIGV
                     CRAYFHVEPGKRLKVVEADAGVYAGDPENAGSADVLCVDLYDHEAASPVLDSDAFYAD
                     CHRLLAPGGAMTVNLFGRDASFERSARRIAAVFGEAQVLSLRPTREGNTIVVALKGTP
                     WPAADVLAARADDIEQHGGLPARKWLKMLRALPQTVPAVPPA"
     gene            8260..10044
                     /locus_tag="PKV56_10950"
     CDS             8260..10044
                     /locus_tag="PKV56_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOX68375.1"
                     /translation="MKSADRPSERPPAGKLDWRKLLDWLREDGLIEREDADRTIKRFG
                     SAQSAQHPLVRLGAAGLVRKGQGGKTLDLEALTEWLAARCGLPYLRIDPLKVDVGRVA
                     DVMSINYAERRFALPLQVGAHDITVATCEPYDTDWVAEIEAHTRKVLKLVVVSPLELQ
                     KYTTEFYTLSRSVRAAIKNGETSTIASFEQLVELGRSNKQLDANDQGVVQVVDWLWQY
                     AFDQRASDIHLEPRRDLSAIRFRIDGVMHTVYQLPPGVMNAMIARVKLLGRMDVIEKR
                     RPLDGRIKTRNPEGDEVEMRLSTLPTAFGEKMVMRIFDPDTTVKPLEALGFGAHDAKR
                     WEALVARPNGIILVTGPTGSGKTSTLYSTLRRLATEEVNVCTIEDPIEMIQPAFNQTQ
                     VQPALDFGFAEGVRALMRQDPDIIMVGEVRDLETAEMAIQAALTGHLVFSTLHTNDAA
                     SAITRLVDLGVPPYLIGATVIGVLAQRLVRTLCPACKQPDEALTAEDLADFVKPWRLN
                     GKAKPYKPVGCLECRMTGFRGRCGLYELLSVTDAARACIQPVPDVTRLRQQALQDGMR
                     PLRLAGAMKVAEGVTTLQEVMSATPAWE"
     gene            10041..10958
                     /locus_tag="PKV56_10955"
     CDS             10041..10958
                     /locus_tag="PKV56_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX68376.1"
                     /translation="MNAPAPEPASPVAAGAVWPGLLLAFLGAVAFSGKAIIAKLAYRH
                     GVDAITLIMYRMLFALPLFVMLSVWASRGRPALHRRDWMVIAGLGFTGYYLASTLDFL
                     GLQYVSASLERLILYLNPTLVLALGVVLFKRRVTGRQLISLGVSYGGVLLVFGQELKH
                     VGPHAVLGAALVFGSAISYAVYLVLSGEEVRRMGALRLTGLASTVACALCIGQFLLLR
                     PLSAMAVAPEVIWLSVLNALVCTFAPVLMVMMAIERLGAALVAQTGMIGPMSTLLMGV
                     LLLGEPFTAGIAAGTVLVIAGIWMLAKWR"
     gene            complement(10965..11372)
                     /locus_tag="PKV56_10960"
     CDS             complement(10965..11372)
                     /locus_tag="PKV56_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68377.1"
                     /translation="MPTSTSTPKTRRAAPKAPAATAKAPIAKPASTPKSAAPQPKKPA
                     VKRVAKGSAPASAKPVKVKLKLVRDSFTMPQTDFELIDVLKQRALGFRHPAKKSELLR
                     AGLQVLADLPPAQLEAALSRIPSLKPGRPKKAD"
     gene            11585..13630
                     /gene="arsA"
                     /locus_tag="PKV56_10965"
     CDS             11585..13630
                     /gene="arsA"
                     /locus_tag="PKV56_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HOX68378.1"
                     /translation="MQISVLGSGCSKCRSTVSMIERAASAAGLEIDIVKVEDPQAIRS
                     SGVGHTPAVVIGGRVVHSGGIPSHEEVLSWLRPDLPGFLDHPTRHLFFTGKGGVGKTS
                     LSTAAALKLADAGSKVLLVSTDAASNLDEMLGIELRNTPTPVPGAPGLSVLNIDPDAA
                     AESYRRRVIAQMSAESSETELATVREQLSGACTTEIASFDEFASLLSGDAERFDHIVF
                     DTAPTGHTLRLLSLPKAWSGFLDGNDRGASCLGPHSGLKMQEARFKAALEALGDPALT
                     TVVLVTRPDAGAVAEAARTSEELRALGLNNQRLVINGVFHARDRTDAVACAIEALGLQ
                     ALSDMPPALRALPQDRVPLRPFDTVGLAALRSLLGAQRDVIESPSVLVGAPIEPAEHF
                     PGLDALVDDLATAEHGLIMVMGKGGVGKTTVAAALALGLVQRGKSVHLSTTDPAAHVA
                     GTLAGEVPGLHVSRIDPKAETQHYIDKIMAAKAPALDAHEQALLLEDLRSPCTEEVAV
                     FHAFSRIVSEGRSAFVVLDTAPTGHSLLLMDATGAYHRQMMREFEGRSTARIVTPLMR
                     LQDAAYTKIILVTLPEVTPVSQAAALQDDLRRASIEPHAWVLNKSVVAAGTHDPLLSA
                     RLAGERRQMARLTAGLAKRLYVLPWLARPPIGLTELSKLVSRPADAAPASQQVQMPA"
     gene            complement(13730..14032)
                     /locus_tag="PKV56_10970"
     CDS             complement(13730..14032)
                     /locus_tag="PKV56_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOX68379.1"
                     /translation="MKKLTTAPNLAIATLWADLLCHAGVPTTVQRAYNSSLAGQIPPD
                     QTLPELWLTDGGNFEHAQRLLDELQHPVSRRWTCTACGETIDGPFEQCWNCGAAMT"
     gene            14117..15121
                     /locus_tag="PKV56_10975"
     CDS             14117..15121
                     /locus_tag="PKV56_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HOX68380.1"
                     /translation="MPATRSISTRAIRIDQPGGSEAMRLIEVPIGEPGPGEIRIRHRA
                     CGLNFIDVYHRTGLYPLPLPASLGMEAAGIVEAVGEGVTHLHAGDRAAYASTPPGSYC
                     EARVMPAKCVVKLPDGIDFETGAAMMLKGLTAQYLLKKTLPQGGLQAGDFVLFHAAAG
                     GVGLIACQWARALGLRLIGTAGSDEKCALALQHGATHAINYRREDFVARVKDITGGRG
                     VKVVYDSVGLDTFEKSLDVLQPFGLLANFGNASGKAPPVDLGVLAAKGSLYVTRPTLF
                     THIATREATQAMADDLFAVVGSGEVHIPVERRYALADVAQAHRDLEARLTTGCSVLLP
                     "
     gene            complement(15152..15952)
                     /locus_tag="PKV56_10980"
     CDS             complement(15152..15952)
                     /locus_tag="PKV56_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOX68381.1"
                     /translation="MNAQTDIDSPADTARPEAPITAFAVPLGHLDWHAPAQWLKLGWR
                     DFTRAPALGLFYGACFTGMGWLLLYVFEHAAAYTLALSAGFLLMGPFMCLGLYEVSRR
                     LEAGDRPDFISSLTAWKTRMSTMGIFGGVLLIVEMLWGRTSLIVFALSFNGMPDFKGS
                     LLALLNPANIGFIVTYVVVGSLFAGLIFSISVVAMPMILDRHTDAITAGLTSLRLCLT
                     HPGVMLLWGAVLTVIVVLAMLPGFFGLLLAGPVIGHASWHAYRAAVPR"
     gene            complement(16028..16543)
                     /gene="lspA"
                     /locus_tag="PKV56_10985"
     CDS             complement(16028..16543)
                     /gene="lspA"
                     /locus_tag="PKV56_10985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOX68382.1"
                     /translation="MASSKAAGSSLLPWLALALLVFLLDQLTKTLILGSFQLGDSHTV
                     TSFFNIVRVHNAGAAFSFLAGASGWQRWFFVGLGLVATVFIVWMLKQHGGQRLFSLAL
                     SLVLGGALGNVIDRLLHGHVIDFLQFHWNWLAPLFAGGYFPSFNIADSAITVGATLLI
                     VDELRRVRKAS"
     gene            complement(16545..>17945)
                     /locus_tag="PKV56_10990"
     CDS             complement(16545..>17945)
                     /locus_tag="PKV56_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX68383.1"
                     /translation="DWCISRQRNWGVPLPFFLHRDSGELHPQTLEFMDRAAALVEQGG
                     VEAWAQLDPSDWLGDEAAKYTKSTDILDVWFDSGSTFNHVLHGSHDGAGQPDGPEADL
                     YLEGHDQHRGWFHSSLLIACAIDGRAPYKGLLTHGFATDGQGRKMSKSLGNVVAPQSV
                     SDKLGAEIVRLWVASTDYSGDLNIDDKILARVVDAYRRIRNTLRFLMANTSDFDAATD
                     AVPLADMLEIDRHALARAAQFQAEVLAHYEVYEFHPVVAKLQVYCSEDLGSFYLDVLK
                     DRLYTTAPNSHARRSAQTALWHITNAMLRWMAPFLSFTAEEAWPIFAPGRSSSIFIET
                     YSDTTGWDDAALLAKWSRIREVRELANKQIETVRAAGQLGSSLQATIAITANAEDHAL
                     LASLGDGLKFVTITSEATLAPGAELAISVTPSTAVKCERCWHYRDDVGHDAAHPTICG
                     RCTSNLYGAGEVRRVA"
BASE COUNT         3100 a         5864 c         5919 g         3062 t
ORIGIN      
        1 tgagccctgt cgtgggtcgg ccgctctggc cccaccgccc atcaccgcgt tttcgattcc
       61 agaacccttt ctgcacatga aattcctgtc tctcaagctc ggcggtaggg cctgcgccct
      121 tctgctggtc agcagcgcga tggcgggcgg ctcgcctccg cccgacctgc ttgaagcccc
      181 agccgtggtg gctgctccgc cccgcagcac cccgttcgac ttgagcgatc cggaccgcat
      241 caaggctggc aagcagcgat tccattcgac ttgcgcagag ttctgccatg ggcacgcccc
      301 gccgctcttc atcggccgcg cggacctgcc tcccaggtac gccttcgaca tgatttccaa
      361 cggcggcgga tcggccacac ccatgccgcc gtggggttcg gtatttactc acgacgaaat
      421 atgggagttg gttgcatacc tgaaatatct gggtacgcaa aagctttagt tgaaaggccc
      481 gccgattctt ggatcgacgg gctttttaac tctggccacc tgttgctttg gatcgaaggt
      541 atttcattaa tttgggtagg tactggttat cttgcatccg ctgtcgcgag gtatcaaggt
      601 gactttctgt cgagcggcca tgcaggaatg cgggttcgcg cgggatctgt tcgagcgagg
      661 cggctcggtt cctacgatgg tgggtctcga aggcacacgg catttcgcga cgtctgtcgc
      721 ctccggcgcg gcatggcgca agacgaagag cctttttggc gcgcccgcaa gccagcagac
      781 cgacccccgc actctgtcgc agcaagccca tgtgcagcga catatgtgat gtcacaggtg
      841 tcgccggagt gacaggtgaa aaccctctgt cattgcctca tcgttttgtt gacgtctgaa
      901 tcggggcaag tcggatttgg caacccgagt ttcacgaacg gcgctttgga gtcgtttggt
      961 gcccgcaaat gcgacaactg aaactctgca tgtacacgga tgaaacagtt ggcacagttg
     1021 attcaccttg gcgtcactgc gcgttatccg ttctcaccag tgaaaacgcg ttgccatgcg
     1081 gcgcgcaggg acttggcaca aatggtgcgg tcagagcagg tcgagatcag tccaaccgga
     1141 aggtcttggc tctggcggac accgcccggg tgtcgcgcac aacaaatcca accctatgag
     1201 aaggagacaa catgggtcac caattcttca acctcaagcc agcggccgcg ggacggctgc
     1261 tcgtgcttgc agggttcatg gcaagcgccg gcctggcctc tgcacagtcc agtgtgcagg
     1321 tgttcggcat catcgacgcc ggcatcctga cgcagaccaa cagcgctgac agcaagaaga
     1381 ccaagacacg cctcgagacc agcggattgc gccagagcgt ctggggcctg aagggcacgg
     1441 aagacctggg tggcggcctg aaggcgttct tcaacctcga atcgcacttc gacaccgaca
     1501 cgggtcacat ccacggcacg ggcgactcga accagagcgg cacgatcctc ttccgacggc
     1561 aagccaacgt cggcctgacc ggcgactggg gcacggtcat cctgggtcgc cagtacggtc
     1621 cggctcttct cgcgcacatc ggcaccgagc cgcgcgcctt caaggaacag ttctcgaacc
     1681 tgtatgcgtg ggcctacaac cagctggacg gcttcgctgg cccaggtgcc gcgggcaaca
     1741 ccaacgacgt cggcatcttc atgagcaatg cggtccagta ccgcaacaac ttcggtccgg
     1801 tgaccgtcgg tgtgctgtat gccttcggcg aacagtccaa gaagatctcg cgcggcaaca
     1861 cgttcgcgat tggtgtcaac tacaacggtc cggtcgtgct ctccggttcg taccaggtca
     1921 tgaaggacaa ggccagcggc gaagacatca tcgagcacta cggcctgggt gccgcggttc
     1981 cgttcggtga cttcaccttc aagctcagct ggctgcgcgg cgagaactcc ggcgccgatg
     2041 gcgtcgagac atccaaggtc gatggcatca gcggcggtgt cgactggaag tggagcccgt
     2101 cgaacagcgc caccctggcc tattacgaca acaaggacaa gaaccagagc agcaaccaca
     2161 cgaagaacat catcatcagc aacgacctgt tcctgtcgaa gcgcacgacg ttctatacgc
     2221 aggtggcaat tgttgacgca ggcaagggtt ccacgggtat cgctgccttc aagaccagcg
     2281 ttgttgccga tgcgagctat ctgccgaaca agacgaccac gttcctgaac gtcggtatca
     2341 accacacctt ctaaccgttc tccacacgcc acccccaaag gtggattggc gctcaccagt
     2401 atccggcccg gtcactggtg agcgcttttt tctgcgcccg catctttcgc catctgtcgc
     2461 gttgcgggct acacgaagga gtcacccatg ttccattccc gtcgtccagc cgcgttgcgg
     2521 cttcatccgc tcgtggcctg catcggcctc ggcctgttgg tcacgtcggt ctgtcgtgca
     2581 gaggacccgg agccgccgct cggtgtcaac gtgctcgaga acaccttcaa atggcgcccg
     2641 aactacgtca gcgacgacat gctgatgcac gccgacaagg acgccaacaa ctggctgcac
     2701 tacggcaagg actaccagaa cacgcgattc agcgcgctca cgcagatcac gaccgagaac
     2761 gtgaagaagc tggtgccgaa atggaacctg tcgttcggcg tcaacgaggc gcaggacagc
     2821 cagaccacgg tgatcaacgg ccgcgtgttc gtgacggcaa gccagaacaa ggtgtttgcg
     2881 cttgacggcg tcaccggcaa gatgctgtgg aagtacgagc gttcgctgcc tggcgatctc
     2941 ggccccaagc tgtgctgcga tgcggtgaac cgtggcgtcg cgctctacaa ggacatggtc
     3001 ttcatggcca ccctcgacac ccatgtcgtc gcgctgaaca gcacgaccgg cgcggtggtc
     3061 tgggaaaaga agatgggcga ctacaagtcg ggcgagatct acacctcgat gccgctggtc
     3121 gccaagggca tggtcatcgt cggcaattcg ggtggtgacg tcggcggcaa cgtcgggcgc
     3181 atcaccgcgc tgaacccggc gaatggcgag gtcatctggg aaaccaagac gcggccgacg
     3241 agcgccgacc atccccaggc caagacctgg gccaacgatt cgtggaagac gggtggatca
     3301 tcggcgtggc tgaccggcac ctacgacgcg aagaccgaca ccgtgctgtg gggcgtgggc
     3361 aacccgacgc ctgacttcga cccgagcgtg cgcaagggcg acaacctcta cagcaactcg
     3421 acactggcgc tcgatgccag caccggcaag atcaagtacc acttccagta cacgccgaac
     3481 gactcgtggg actacgacgg caacaacgaa gtcatcatgg tcgacgacga aaagggccgc
     3541 aaggtctggg tgcacggtga ccgcaacggc cacctgtact cgatcgatcg caccaacggc
     3601 aagtgcaact gggttgttcc gattgcccgc gtcaactggg tcaccggctt cggcgagaac
     3661 tgccggccga tcgtcaatcc ggacaaggtg ccgcgctacg acaagatcgt gaccgacatc
     3721 gcgcctatcc tcgacggcgg caaggaatgg cacccggctg cctacagccc ccggaccaag
     3781 ctggtctaca tcccgttcat cgacagttcg atggacatcc aggccaagaa gaccgagtgg
     3841 aagcacggcg agtggttcct gtcgtcgcgt gtcctgaagg ccaacccata caccggtggc
     3901 gtgaaggctt tcaatgcgac caccggcgag cagatctgga cacgtccgca aagcactccg
     3961 gccaccagcg gattgctggc caccgccggc aagctggtgt tctcgggtga cgccgaaggc
     4021 tggttcagcg cactgaagga cgacacgggc gaagagatgt ggcgcttcaa cgtcggctcc
     4081 ggcatccacg gcaaccccac gaccttcacg gccgatggca agcagtacgt ggcgatcgtc
     4141 tacggaccgg gcggtggcag cctgctgccg ctgatctacg acgagctgat gaagcgcaac
     4201 aaccggggcg gcggcctgat ggtcttcgga ctctccgatt gatgcactga tccatggacc
     4261 ggggtgcggc gcaggtcgcg ccccgtccgg acacgccccc atgaagtacc tcagaagatt
     4321 tgcccaggcc gcggccttgt tgctggtcgc cggcggattg cgagccgcgg aggccgatgc
     4381 gccggcctac ctgaacgtgt gtgtggatga cgacaacccg ccgttctcgt ccgaggcccg
     4441 accggaacgc ggtatcgatg tcgaggtcgc gcaggccatc gccggccagc tcggccgacc
     4501 gttgaagctg gtgtgggcca cggtgcccaa ccgcggcggc atcggcaagg ccctgcgtca
     4561 gtcgctgtct gccgggaagt gcgacgcgta tctcggcatc ccgcaggatc ccgatctggc
     4621 cagggagctc gccgagcgca agctcgtcac gtcgcctccc tatctcgttc ttggctatgt
     4681 actggtcgct gcgccaggca aacccgtgcc gacagccgct gcgctgcgcg gggctcgccg
     4741 cgtcggtgcc gtcacggcca cgccggccga tctgtacctt tatcgcaagc agctcggtcg
     4801 cgtcccctac cctggcagcg ccgcattgat cgaggcgctg aaggccggag aagtcgatct
     4861 ggcactcgtg tggtcgtcct cgctgactgg cgacgcaggc aagagcgtgg tggctgctac
     4921 cgaggcggtg gacgatgccg acctgtacac cggcatgacc gtggcgaccc gcactgctga
     4981 tacggctctg gcgaacgagc tcgcgaaagc gatcgatgcc ctgcggagca atggacgcat
     5041 cgatacgatc atgcagcggt acggcctgcc gcgcgtatca cgaccctgac tccacgtccg
     5101 cctgaatcag aagatgtctc aaatcaccaa cttgcgtgtt cggctcgcca gcgcgctcct
     5161 ggtctcttcc ctgtcggggt ccacactggc ttttgggccg gcgccagcct cgacctccgg
     5221 ggcaggcagt gtcacccttt cgcaggccac cgagtcagcg cctgcccccg cgcctgtggc
     5281 ttccccggat ttcgagaagc agggcgagga gctgttcggt cgcaactgcc agctgtgcca
     5341 caactcgcgc ggcaagggtg gcaaggctcc ccagctcttg cggggggctt ggggccccgg
     5401 cggcgccaac agcgatgagt tcatgttcaa caccatcaag aacgggcgcc ccaacacgca
     5461 gatgggcggc ttcaacggct cgctcagcga tgcggagatc cgcatcatcg tcgccttcct
     5521 gcgttccgaa tcggtgcgcg ccaaggccaa cgaccgcaag acggtgagca cagactacga
     5581 accttggtga gttcggcgcc aaagccggac tttctgcaga ttcgtccatg cccggtcggc
     5641 gccgagttct cggtccggcg agcatgcgct gcacgtactt acctcctcac tcccgttatt
     5701 gaaaggtttt cgatgtccaa gtcatctgca ggctgcctcg cggccgcttc gctcatgctg
     5761 tgcgctgctg cagcccaggc cctggatcgc gtgccggtcc tgtcgctcga ggtcgcgcag
     5821 accatgtcgc gcacctgcga aaccctcgcg aaggtgaagg gctggcgcat gaacatcgcg
     5881 gtggtcgatg ccggcaccaa cctcattgcc ttcagccgca tggaccaggc atttctcggc
     5941 agcaccgaca tcgcgatcag caaggccaag ttctcgggca gtttcccgtt ctcgacgcgc
     6001 tatgccggtg aactgacctt cggcaaggac ggcaagccgg ctccggtgcc cggtctggca
     6061 gacattccgg gcgtggtgac cttcgccggt gggctgccca tcatggttgc aggtgtccag
     6121 gtgggcggca tcggcgtcag cggtggttcg ccagatgaag acgagcaatg cgcggccgcg
     6181 gcgatcgatg ccgtcaagga ctcgctgaag tgacctgagg ccggctcgag cctgccgcac
     6241 atcgcgacag gctgggcatg gcgggccctc ccgggcccgc ggttcgcggc atcgatcagt
     6301 cgatgctgat cacgaccttg ccgaagtgcg atgcgccttc gaggtgggcc agtgctgcgc
     6361 gtgcttcggt gaacgggaac acgcgatcga tcaccggctt gatctggcgc gcgctgatca
     6421 ggcggttcat cgactcgaac atgtcgcgcg agcccacgaa cacgccgcgc agcagcgtgg
     6481 tgttgaacag gatggccagc gggtcgatct gacctccggt cagcacgccg atcaggtgca
     6541 cggccccgcc gtggcgggcc gctgcgatcg agcgctgcag ggtgcctggt ccaccgacct
     6601 cgaccacgtg gtcgacgccg cggcctccag tgacttcgcg cacgcgctgt tcccagtccg
     6661 ggttcgtctt gtagttgatc agatggtcag cacccagcgc cttggcctta gccagcttgg
     6721 catcgctcga cgaggtggcg atcaccgtgg cgccagaggc cttggcgaac tgcagcgcga
     6781 acatcgacac accgccggtg ccgagcacca gcaccgtttc gccggggacc agttgccggc
     6841 cctcgtacag cgcgttccag gcggtcagcg ccgcgcaggg cagggtggcc gcttccgcat
     6901 cgctgaggtg cgctggcgtg gcgaccagtg cctcctcggg gaagacgcgg tattcggcca
     6961 gcacgccggg caccgatccg cccagcgcgt tttcatagtg cttgggggtc atctcgccgc
     7021 tgagccaggt ctcgaagaag cagccggtga cgcggtcgcc actggcgaag cgcgtgacgc
     7081 ccggtccgac ttcggccacc acgccggcgc cgtcagatcc agggaccagt ccttcggatt
     7141 tcatgcccgg gtagcggccc cagacaatga tgaggtcccg gtagttgagc gaggccgcct
     7201 tcatgcgcac cagcacctgc cccggtcccg ggcgcgggac ctcgcgctcc tgcagcgtca
     7261 ggctgtcggc gttgttttcc ttgaggtacg tatagcagcg catagtgatg tctccgatgt
     7321 gattgttcga atcagcgaac cctgggccga agccggtttt cgatgatgcc atgcatgagc
     7381 aaaacgcgcg ccgcgcttcc gcggcactcg atggcaacat ccgtcgcacg atgaagaaga
     7441 aaaagcagac ccccgaactg gctcccgcca cgatctccga agccgacggg gtgcgctacc
     7501 tgcacctcgg aacgccctgg gttcagggag ccatgcgcat ccgcaagccg ctggcgatcg
     7561 atctcgaata cgttcagcgc atgatggtct ggatgctgct gcggccgagc agcgactggc
     7621 ccgagtgcca tgcggtccag ctcggcctcg gagccggcgc gatcacccgc tactgccacg
     7681 gcgtgctgcg catgcggtgc acggcagtcg agttgaacga caccgtgatc ggcgtgtgcc
     7741 gggcctattt ccatgtcgag cccgggaaac ggctgaaggt ggtcgaggcg gatgccgggg
     7801 tctacgctgg cgatcccgag aacgccggca gcgccgacgt gctgtgtgtc gacctgtacg
     7861 accacgaggc cgccagcccg gtgctcgaca gcgacgcgtt ctacgccgat tgccaccgcc
     7921 tgctggcacc gggcggtgcc atgacggtca acctgttcgg ccgcgatgcc agcttcgagc
     7981 gcagcgcgcg ccgcattgct gccgtgttcg gcgaggccca ggtgctgagc ctgcggccga
     8041 cgcgcgaagg caacaccatc gtcgtcgcgc tgaaaggcac cccgtggccg gccgccgacg
     8101 tgctcgctgc gcgggccgac gacatcgagc agcacggcgg gctgcccgcg cgaaaatggc
     8161 tgaagatgct gcgagcgttg ccgcagacgg tcccggccgt gccgccggct tgaaaccttc
     8221 ccgtcgacga atccgccaca cccctcaaag acgcactcca tgaaatccgc tgaccgtcca
     8281 tccgaacgcc cgcccgccgg caagctcgac tggcgcaagc tgctcgactg gttgcgtgaa
     8341 gacggcctga tcgagcgtga agatgccgac cgcacgatca agcggttcgg ctccgcccaa
     8401 agtgctcaac atccgctggt tcgcctgggt gccgccgggc tggtgcgcaa gggccaaggg
     8461 ggaaagacgc tggatctcga ggcgctgacc gagtggctgg ccgctcgctg cgggttgcct
     8521 tacctgcgca tcgatccgct gaaggtcgat gtcggccgtg tcgccgacgt gatgtccatc
     8581 aactacgccg agcgccgctt cgcgctgcca ttgcaggtcg gcgcgcatga catcacggtg
     8641 gccacctgtg agccctacga caccgactgg gtggccgaga tcgaggcgca cacccgcaag
     8701 gtgctgaagc tggtcgtggt cagcccgctt gagctgcaga agtacacgac cgagttctac
     8761 acgctgtcac gctcggttcg tgcggccatc aagaacggcg agacatcgac gatcgccagc
     8821 ttcgagcagc tggtcgaact cgggcgcagc aacaagcagc tcgatgccaa tgaccagggc
     8881 gtggtgcagg tcgtcgactg gctgtggcag tacgcattcg atcagcgcgc ctcggacatc
     8941 cacctggagc cacgacgcga cctgagcgcg atccgcttcc gcatcgatgg cgtgatgcac
     9001 acggtgtatc agctgccgcc tggcgtgatg aacgcgatga tcgcgcgcgt caagctgctc
     9061 ggccgcatgg acgtgatcga gaagcggcgt ccgctcgacg ggcgcatcaa gacacgcaac
     9121 cccgaaggcg acgaggtgga gatgcggctg tcgacgctgc ccaccgcctt cggcgagaag
     9181 atggtgatgc ggatcttcga ccccgacacc accgtcaagc cgctcgaggc gctggggttc
     9241 ggcgcccacg atgcaaagcg ctgggaagcc ctggtggcgc ggcccaacgg catcatcctg
     9301 gtgacggggc cgaccggttc gggcaagacc agcacgctgt actcgacgct gcgccggctc
     9361 gccaccgaag aggtcaacgt ctgcacgatc gaggatccga tcgagatgat ccagcctgcg
     9421 ttcaaccaga cccaggtgca gcccgcgctc gattttggtt tcgccgaagg cgtgcgcgcg
     9481 ctgatgcggc aggacccgga catcatcatg gtcggcgagg tgcgtgacct cgagacggcc
     9541 gagatggcga ttcaggccgc gctgaccggc cacctggtct tcagcacgct gcacaccaac
     9601 gacgcagcgt ccgcgatcac gcggctggtc gatctgggcg tgccgccgta cctgatcggg
     9661 gccaccgtga tcggtgtgct cgcgcagcgg ctggtgcgca cgctgtgccc ggcctgcaag
     9721 cagcccgacg aggctctgac ggccgaggac ctcgcggatt tcgtcaagcc ctggcgcctg
     9781 aacggcaagg ccaagcctta caagccggtg ggctgcctcg aatgccgcat gaccggcttc
     9841 cgcgggcgtt gcgggctgta tgaactcctg tcggtgaccg atgcggcgcg tgcgtgcatc
     9901 cagccggttc ccgacgtcac ccgcctgcgt cagcaggcac tgcaggacgg catgcggccg
     9961 ctgcgtctgg ccggtgcgat gaaggtcgcc gaaggcgtga cgaccctgca ggaagtgatg
    10021 agcgccacgc ccgcgtggga gtgaacgcgc ccgcacccga accagcatcg cccgtagctg
    10081 cgggggcggt ctggcccggg ctcctgctgg ctttcctggg cgcggtggcg ttctccggca
    10141 aggccatcat cgccaagctc gcctaccggc acggcgtcga cgcgatcacg ctgatcatgt
    10201 accgcatgct gttcgcgctg cccctgttcg tgatgctcag cgtgtgggcc agccgaggca
    10261 ggccggcgtt gcaccgtcgc gactggatgg tgatcgccgg gctgggcttc accggttact
    10321 acctggcgag cacgctcgat ttcttgggcc tgcagtacgt cagcgccagc ctcgagaggc
    10381 tgatcctgta cctgaatccc acgctggtgc tggcgctcgg tgtcgtgctg ttcaagcgac
    10441 gtgtcaccgg gcggcagctg atctcgctgg gcgtcagcta cggcggcgtg ctgctggtat
    10501 tcggccagga gctgaagcac gtcgggccgc acgccgtgct cggtgcggcg ctggtgttcg
    10561 gcagcgcgat cagctacgcg gtctacctgg tcctcagtgg cgaggaggtg cggcgcatgg
    10621 gggcgctgcg cctcacgggc ctggccagca cggtggcctg cgcgctgtgc atcggacagt
    10681 tcctgctgct gcgcccgctg tcggcgatgg cggtggcccc ggaagtcatc tggctctcgg
    10741 tgctcaatgc actggtgtgc acgttcgcgc ctgtgctgat ggtgatgatg gcgatcgagc
    10801 gcctcggtgc cgcgctggtc gcgcagaccg ggatgatcgg cccgatgtcc acgctgctga
    10861 tgggcgtgct gctgctcggc gaacccttca ccgccggcat cgctgccggc acggtgctgg
    10921 tgatcgccgg catctggatg ctggcgaaat ggcgatgagg ccggtcagtc ggcctttttc
    10981 gggcgcccgg gcttcagcga tggaatgcgc gaaagcgcgg cttccagctg cgccggcggc
    11041 aggtcggcca gcacctgaag gcccgcccgc agcagttcgc tcttcttggc cgggtgacgg
    11101 aagcccagcg cgcgctgctt cagcacgtcg atcagctcga agtctgtctg cggcatcgtg
    11161 aagctgtcgc ggaccagctt gagcttgacc ttgaccggct tggcgctggc cggggcgctg
    11221 cctttcgcga cgcgcttgac ggccggcttc ttcggctggg gagcagcgct cttcggcgtg
    11281 ctggcaggct tggcgatcgg cgcctttgcc gtggccgcgg gcgcctttgg agcggcgcga
    11341 cgagtcttgg gggtgctggt tgaagtaggc atgcggaacc tccgtaatca atgtaaatgg
    11401 tttataccgt ataaatcata aaacagtaaa tgacgggttg cctgctgcct tcgccttccg
    11461 gctgatcccg gtcaagccgg atgcagagag tcgagcgtcc aatccgagac ggcccctgcc
    11521 gggcccgagc cagctccctg agcgagcgca gacggccggc cgcatcaaca acaaggtacc
    11581 cgacatgcaa atcagtgttc tggggagcgg ctgcagcaag tgccgcagca ccgtttcgat
    11641 gatcgagagg gctgcgtcgg cggccgggct cgagatcgac atcgtgaagg tcgaggatcc
    11701 gcaggccatc cggagttcgg gtgtcgggca cacgcccgct gtcgtcatcg gtggccgggt
    11761 cgtgcacagc ggagggattc catcgcacga ggaagtgctg agctggctgc gtccggactt
    11821 gccgggattc ctggaccacc cgacgcgaca cctgttcttc acgggcaagg ggggggtggg
    11881 caagacttcg ctgtcgacgg ctgccgcgct gaagctggcc gacgcgggca gcaaggtgct
    11941 gctggtcagc accgatgcag cgtccaacct cgacgagatg ctggggatcg agcttcgcaa
    12001 cacgccgacg ccggtgcccg gcgcaccggg cctgtcggtg ctgaacatcg accccgatgc
    12061 ggcggcagag tcctatcgac ggcgggtcat cgcgcagatg agcgccgaat ccagcgagac
    12121 cgaactggcg accgtgcgcg agcagttgtc cggcgcctgc accaccgaga tcgcctcgtt
    12181 cgacgagttc gcctcgctgc tgtcaggcga tgccgaacgc ttcgaccaca tcgtcttcga
    12241 cacggcaccg acaggtcaca ccttgcgctt gctgagcctg ccgaaggcgt ggagcggctt
    12301 cctcgacggc aacgatcgag gcgcctcctg tctgggcccg cattccggac tgaagatgca
    12361 ggaagcccgt ttcaaggcgg ccctcgaggc cctcggcgat ccggccctga ccacggtcgt
    12421 cctggtcacc cggcccgatg cgggcgcggt ggccgaggcg gcgcggacct ccgaggaatt
    12481 gcgtgcgctt ggattgaaca accagcggct ggtgatcaac ggcgtgttcc atgcccgcga
    12541 ccgcaccgac gcggtggcct gcgcgatcga ggcgctgggg ctgcaggcac tgtcggacat
    12601 gccgccggct ctgcgcgcgc tgccgcagga ccgcgttccg ctgaggccgt tcgacaccgt
    12661 ggggttggcc gccttgcgat cgttgcttgg cgcacagcgc gatgtcatcg agtcgccttc
    12721 cgtgttggtt ggcgcaccga tcgaacccgc agagcacttc ccgggcctcg atgcgctggt
    12781 cgatgacctg gccaccgccg aacacggcct gatcatggtc atgggcaagg gcggtgtcgg
    12841 caagaccacg gtggcggcgg cgttggccct cggcctcgtt cagcgcggca agtcggtgca
    12901 cctgagcacc accgatccgg cggcccacgt ggccggaacg cttgccggtg aggtcccggg
    12961 cctgcacgtc agccgcatcg acccgaaggc cgagacgcag cactacatcg acaagatcat
    13021 ggcggccaag gcccccgcgc tcgatgcaca cgagcaggcc ttgctgctcg aggacctgcg
    13081 ttcgccttgc accgaggaag tggccgtctt ccatgcgttc tcgcgcatcg tcagcgaggg
    13141 tcgcagtgcc ttcgtcgtgc tggacaccgc gcccaccggg cattcgctgc tgctgatgga
    13201 tgcgacgggc gcctaccacc gacagatgat gcgggagttc gaggggcgca gcaccgcacg
    13261 catcgtcacc ccgctgatga ggctgcagga cgccgcgtac acgaagatca tcctggtgac
    13321 gctgcccgag gtgacgccgg tgtcacaggc tgcggcgctc caggacgatc tgcgccgcgc
    13381 cagcatcgag cctcacgcct gggtgctcaa caagagcgtg gtcgctgcgg gaacgcatga
    13441 cccgctgctg tcggcgcgcc tggccgggga gcgcaggcag atggcgcgtc tgaccgccgg
    13501 gctggcgaag cgtctttacg tgctgccctg gctggcacgc ccgcccatcg gcttgacgga
    13561 gctgtcgaag ctggtcagcc ggccggccga cgcagcaccc gcgtcgcaac aagttcagat
    13621 gcctgcctga cccgcctgcg ccgccaggga tctgttgcgg cgatgtgccg ttcgcctcgg
    13681 atggcgcttg cggctcccgc cgccgtggac ccgagccggg gcgacgcctt caggtcatcg
    13741 cagcaccgca gttccagcac tgctcgaacg ggccgtcgat cgtttcgccg caggctgtgc
    13801 aggtccagcg acgcgacacc ggatgctgca actcatcgag caggcgctgc gcgtgctcga
    13861 agttgccgcc gtcggtcagc cacagttcgg gcagcgtctg gtcgggtggg atctgccctg
    13921 ccaggctgct gttgtaggcg cgctggactg tcgtcggcac cccggcgtga cacagcagat
    13981 cggcccacag cgtggcgatg gcgaggttgg gggcggtggt gagcttcttc atggccgggc
    14041 gagactgtcg acccatggcg agtgctcgaa ttgggcttag ggcacgatag cgcactgttt
    14101 tcacgaggag cacccgatgc cggccacccg ttcaatcagc acccgcgcca tccgcatcga
    14161 ccaacccgga ggctccgagg cgatgcgtct gatcgaagtg ccgatcggcg agcccggccc
    14221 gggcgagata cgcatccggc accgggcatg cgggctcaac ttcatcgatg tctaccaccg
    14281 caccgggctc tacccgctgc cgctgcctgc atccctcggt atggaagcgg ccggcatcgt
    14341 cgaagcggtg ggggaagggg tgacgcacct gcacgccggg gatcgagcgg cttatgccag
    14401 taccccgccg ggcagctact gcgaggcccg cgtgatgccg gccaagtgcg tcgtgaagct
    14461 gcctgacggc atcgatttcg agaccggcgc cgcgatgatg ctcaagggcc tgaccgcgca
    14521 gtacctgctg aagaagacgc tgcctcaggg cggcttgcag gcaggcgact tcgtgctctt
    14581 ccacgcggcg gccggcggtg tcggcctgat tgcatgccag tgggcccggg cgctcggcct
    14641 gaggttgatc ggcacggcgg gcagcgacga gaaatgcgcg ctggccttgc agcacggcgc
    14701 aacccacgcc atcaactacc ggcgcgagga tttcgtcgca cgcgtcaagg acatcaccgg
    14761 cggccgcggc gtcaaggtgg tctacgactc ggtcgggctg gacaccttcg agaagtcgct
    14821 cgacgtgctg cagcctttcg gcctgctggc gaatttcggc aatgcatcgg gcaaggcgcc
    14881 gccggtggat ctgggcgttc tggcggccaa gggatcgctg tacgtgacac gccccacgct
    14941 gttcacgcac atcgccacgc gcgaggccac gcaggcgatg gccgacgatc tgttcgccgt
    15001 cgttggcagc ggcgaggtgc acatcccggt cgaacgtcgc tacgcgctgg ccgatgtcgc
    15061 ccaggcgcat cgcgacctgg aagcgcggct gacgactggc tgcagcgtgt tgctgccctg
    15121 acggtcaggg cgcagccatc gacgcgccga ttcagcgagg gaccgcggcc cggtaggcgt
    15181 gccaggaggc gtggccgatg accggccccg ccagcagcag cccgaagaac cccggcagca
    15241 tcgccagcac cacgatcacc gtcagcacgg caccccacag cagcatcaca ccggggtggg
    15301 tgaggcacag ccgcaggctg gtcagtccgg cggtgatggc gtcggtgtgc cggtcgagga
    15361 tcatcggcat cgccaccacg ctgatggaga agatcagtcc tgcgaagagc gagcccacga
    15421 ccacgtaggt gacgatgaat ccgatgttgg ccgggttcag cagcgccagc agcgacccct
    15481 tgaagtcggg catgccgttg aagctgagcg cgaacacgat cagcgaagtg cgcccccaga
    15541 gcatctcgac gatcagcagc acgcccccga agatgcccat ggtgctcatc cgtgtcttcc
    15601 aagcagtcag cgaggaaatg aagtccggcc gatcaccggc ctcgagccgg cggctcacct
    15661 cgtacagacc caggcacatg aacggaccca tcagcaggaa gcctgcgctc aatgccagcg
    15721 tgtaggcagc ggcatgctcg aaaacgtaaa gcagcagcca gcccatgccc gtgaagcagg
    15781 cgccgtagaa cagcccgagg gcgggggccc gtgtgaagtc gcgccagccc agcttcagcc
    15841 actgggcggg cgcgtgccag tcgaggtggc ccagcggcac ggcgaacgcc gtgatcgggg
    15901 cctcaggtct ggctgtgtcg gcgggggagt cgatgtcggt ctgggcattc atggcagcgc
    15961 agtgtgcagg cgcaatccgc gcattgcacc ccgcacttac atcaatgcgc cggcctgctg
    16021 acggtgttca gcttgccttg cgcacccgcc gcaactcatc gacgatgagc agcgtcgccc
    16081 cgacggtgat cgcgctgtcc gcgatgttga agctgggaaa ataaccgccc gcgaacagcg
    16141 gtgccagcca gttccagtgg aactgcagga agtcgatcac gtgcccgtgc agcagccggt
    16201 cgatcacgtt gcccagcgcg cctccgagaa ccagcgacag cgccagactg aacaaccgct
    16261 gaccgccatg ctgcttcagc atccagacga tgaacacggt ggccaccagt ccgagtccga
    16321 cgaagaacca gcgctgccag cccgaggcac ccgccaggaa gctgaaggcg gcccccgcgt
    16381 tgtgcacgcg cacgatgttg aagaacgacg tgacggtgtg gctgtcaccc agctggaagc
    16441 tcccgaggat cagcgtcttg gtcagctgat cgagcaggaa taccagcagt gccagcgcga
    16501 gccacgggag caggctggaa cctgccgcct tgctgctcgc catgtcaggc cacccgacgg
    16561 acttcgccgg cgccgtacag gttgctggtg cagcgcccgc agatcgtcgg gtgggccgca
    16621 tcgtgtccga catcgtctcg gtagtgccag catcgctcgc acttcaccgc ggtcgatggc
    16681 gtcacgctga ttgccagttc ggcaccaggc gcaagcgtgg cctcggaggt gatcgtgacg
    16741 aacttcaggc cgtcgcccaa ggacgccagc agcgcgtggt cctcggcgtt cgcggtgatg
    16801 gcgatcgtgg cctgcagcga cgagcccagt tgtccggcgg cgcgcaccgt ctcgatctgc
    16861 ttgttggcga gctcgcgaac ctcgcggatt cgcgaccact tcgccaacag cgccgcatcg
    16921 tcccagccgg tggtgtcgct gtaggtctcg atgaagatcg atgacgagcg gcctggcgcg
    16981 aagatcggcc aagcttcctc ggcggtgaag ctgaggaacg gcgccatcca gcgcagcatc
    17041 gcgttcgtga tgtgccacag cgcggtctgt gcgctgcgcc gtgcgtggct atttggcgcg
    17101 gtggtgtaga gccggtcctt cagcacgtcg aggtagaacg agcccaggtc ctcgctgcag
    17161 tagacctgca gcttcgcgac caccgggtgg aattcgtaca cctcgtagtg cgccagcacc
    17221 tcggcctgga actgcgccgc gcgggccagc gcatggcggt cgatctcgag catgtctgcc
    17281 agcggcaccg cgtcggtcgc cgcgtcgaaa tcgctggtgt tggccatcag gaaccgcagc
    17341 gtgttgcgga tgcggcggta ggcatcgacc acgcgtgcaa ggatcttgtc gtcgatgttc
    17401 aggtcgccgg aatagtcggt gctcgccacc cacagtcgca cgatctcggc accgagcttg
    17461 tcgctgaccg attgaggtgc gacgacgttg ccaagcgact tgctcatctt gcggccctgg
    17521 ccgtcggtcg cgaagccgtg cgtgagcagg cccttgtacg gtgcgcgacc gtctatcgcg
    17581 caggcgatca gcagcgatga atggaaccag ccgcggtgct ggtcgtggcc ttcgaggtac
    17641 aggtcggcct ccggcccgtc cggctggccg gcgccgtcgt gcgagccgtg cagcacgtgg
    17701 ttgaacgtgc ttcccgagtc gaaccagacg tcgaggatgt cggtgctctt ggtgtacttc
    17761 gcagcctcgt cgcccagcca gtcgctcgga tcaagctgcg cccacgcttc gacgcctccc
    17821 tgctcgacga gtgccgccgc gcggtccatg aattcgagcg tctgcggatg cagctcgccg
    17881 ctgtcgcggt gcaggaagaa cggcagcggc acgccccagt tgcgctggcg gctgatgcac
    17941 cagtc
//
LOCUS       DAOORV010000074        17661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3895_length_17661_cov_7.335170, whole genome shotgun sequence.
ACCESSION   DAOORV010000074 DAOORV010000000
VERSION     DAOORV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_17661_cov_7.335170"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKV56_10995"
     CDS             complement(<1..264)
                     /locus_tag="PKV56_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68384.1"
                     /translation="MRTLPQLTSRPIRAALILELVLACHCAGAAPAAATAVDALHRQE
                     RATCISGRSNQDRATCLREADAAHAEARRGALQVGSPALTDNAL"
     gene            387..944
                     /locus_tag="PKV56_11000"
     CDS             387..944
                     /locus_tag="PKV56_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOX68385.1"
                     /translation="MRLPEDIASKLLGVPYASLDERTKKVTRSVAGRTHIARNLTRTD
                     DETPSLGQRAADAVASFGGSWTFVGLFALTMLVWVGINALLLIYRGATFDPYPYILLN
                     LFLSMLAAIQAPIILMSQNRQAEKDRATVEHDYEVNLKAELEIMLLHEKIDLLRETQW
                     KELLDIQQEQLRLLSEQVGRKAPGA"
     gene            complement(958..2076)
                     /locus_tag="PKV56_11005"
     CDS             complement(958..2076)
                     /locus_tag="PKV56_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HOX68386.1"
                     /translation="MNPRADADPPAARWPVPGLPLWSIAVPVLALLTLASTWGQPLGI
                     GLGALLAAALLLAVLCAIHHAEVVAHRVGEPYGTLVLAVAVTIIEVALIVSLMLAGGA
                     SANTLARDTVFSAVMIVCNGVIGLCLVVGGLRHGVLVFRVEGTSPALASLGALAGLAL
                     VLPSFTTTTSGPTYSGSQLAFAGFTSLALYSVFVFVQTVRHRDYFLPVGTDDTKAHAP
                     PPAHWIAWTSLGLLLVSLVGVVGLAKALAPALEAGVAAAGAPKAVSGVLIALIVLLPE
                     TGAALRAARGNRMQSSLNLALGSGLASIGLTIPVVAALSPWFPFPLVLGLRPIDIVLL
                     MLTLVVGTLTLGSGRATVMQGAVHLVLFASFLFLTVVP"
     gene            2146..2535
                     /locus_tag="PKV56_11010"
     CDS             2146..2535
                     /locus_tag="PKV56_11010"
                     /inference="COORDINATES: protein motif:HMM:NF022610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HOX68387.1"
                     /translation="MRSASSTRRIRVWVASLAILLGALAPSITHALNSLSGSAFLEVC
                     TAVGSRFGWSVDNEASAPKSGVPVDQAHHCPFCVLHASAPGMPPAPLQGLTLLPLAFH
                     VPELFLAAPRTLNAWTTVQARAPPHHS"
     gene            2757..4670
                     /locus_tag="PKV56_11015"
     CDS             2757..4670
                     /locus_tag="PKV56_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX68388.1"
                     /translation="MKKISPPLFGLALLQMAIGAAYAQGTSAPKSLEKVVVTGSPIIE
                     SSRIDAFGSLTTEVGASQVRDLNALDLSSALRRTPGVIVSRFNPVGSFGGDEGGAVYV
                     RGLGASRPGSEIKTYVDGIPFYMGVWNHSLLDLLPVHGMESISVHKGPQPQRFGNTFA
                     AIDLIPKRAVGDGFGGDVRVSAGSDSTAVEQAALTFRSDATELSLAQGFARSDGHRKD
                     ADGRLANLMLRGSHDLNAQWRIGGMILGSDNKVRDPGEKGRPATKTGSYVTRGTLGSL
                     SLEHRHDQLQGRLQVYDNDGMSNWQNPTAANVRSRFHLSGLRWREELAPWKDGTVVAG
                     LDVDRMSGSVAFNGFTAFDDVSLRLTSPYIGLAQRFELGDRWAVTPSAGVRGYRHNVY
                     GSSSAPHAGVVMENDTVAVRLNASRGLNHPGLDAPLLNAIVPPLGGAPTSWRSLDPER
                     MDHVEAGVHWTPRRGSSIDVSVFHDELKNRYVFAFPPAVAMPSFTNLGNYRVRGLEGV
                     WQQQWTSAWSSFAGLTLLDSSLDDLPYAPKRALSLGLTWQDGPWRVSGDAQSQSRMFV
                     LNKARADGAPNTAHVGGFAVLNLRAAYALSALGPRGEVFIALENLGDRRYAYRPGYPM
                     PGLTGQIGLNYSL"
     gene            4687..5139
                     /locus_tag="PKV56_11020"
     CDS             4687..5139
                     /locus_tag="PKV56_11020"
                     /inference="COORDINATES: protein motif:HMM:NF033672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper uptake system-associated protein"
                     /protein_id="HOX68389.1"
                     /translation="MKSLISTTLMLLQLACVIPSARAADSPDEAGIRQVLMTTFDKPE
                     ARMQVEPIVVQAEHAVAGWIQGERGGRALMRREGRDWSIVLCGGDGLKDPQALAMAGI
                     EPAAARALVQRLNAAEANLTPAKRARFASFEGMVHMDAQGRHPAPGHH"
     gene            5276..6082
                     /locus_tag="PKV56_11025"
     CDS             5276..6082
                     /locus_tag="PKV56_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOX68390.1"
                     /translation="MNMESIQTFLGTTAIDVGLKVLAAIAFWVVGRWMIGRVISLMQA
                     AMNRNHVDPTLTKYLGSIIAIALNIALVLGILGYFGIETTSFAALLAGAGVAIGAAWS
                     GLLGNFAAGAFMLILRPFKVGDFVSIAGIVGTVHELGLFGTTLVTPDNVMTIVGNGKI
                     FGDTIQNFSVLPVRRVERVAQLANGVDPLDAIARFKAAVALIPNISKEMPPEVNLLDF
                     KLEGPQIAVRPYTHTDHYWQVYFDTNEAIVKVCKEAGWPVPAALHQVRQL"
     gene            6153..7811
                     /locus_tag="PKV56_11030"
     CDS             6153..7811
                     /locus_tag="PKV56_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOX68391.1"
                     /translation="MSLARLFLALLLLLIGGASWAQAASDPPAGPVPVAAQTAVAAEA
                     EVVVINRTVTVFRAPYLGVAPERRARRTEAILSDLLDRSVLGAVTIQVEPQGHVLMVD
                     GELALVLTPADVDPLRAQTLEQASAAARDALTQVLSETREGRDARRLLHGAGRSLIAT
                     LVVAVVAWLAWRARRWTTVRLAQRVARSAAAVRVAGTELLHAERLVAFVRLGVAALWW
                     LLALALSYEWLSYVLRQFPYTRPWGEGLTEYLFGVASQLGRGVLGALPDLVIAVVIFL
                     MARGVIAMFRPFFNRLAANDNRQRGWLDADTAGPTRRLFNIGVWVFAAVMAYPYLPGA
                     QSEAFKGVSLLIGLMVTLGGSSLFGQAASGLVLMYSRTLRVGEYVRINENEGTVTEMG
                     VFVTRVRTGLGEELTFPNSLVLGSVSKNYSRAVEGRGYVVDTTVTIGYDTPWRQVEAL
                     LIEAAGRTPGVLRSPAPRVFQTALSDFYPEYRLVCQAVPSEPRPRAEVLSALHASIQD
                     VFNEHGVQIMSPHYLGDPADPKLVPPDRWYAEPARRSEPAAPSA"
     gene            complement(7914..8369)
                     /locus_tag="PKV56_11035"
     CDS             complement(7914..8369)
                     /locus_tag="PKV56_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68392.1"
                     /translation="MKRIFAVVLAVAAIGSASAETSVGVSISIRQPGVYGRIDIGDMP
                     RPALVYAQPVIIAPPPVMYERRPIYLYVPPVHQQDWRRYCGRYAACGQPVYFVRDQWV
                     RERYQQEHPGWDRGRKRGWDRHDGDRRGDRRNDDRGNGNGRGNGHGRDH"
     gene            8648..9067
                     /locus_tag="PKV56_11040"
     CDS             8648..9067
                     /locus_tag="PKV56_11040"
                     /inference="COORDINATES: protein motif:HMM:NF025045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HOX68393.1"
                     /translation="MNKINALLLTSLSLFAATTAFAGESASTRTRADVIAELVAARAA
                     GELPVSGYETSYTVNTRSAASTLTRADVQAEYVRARQAGELEEVTGYSSVANVATSPS
                     RLTRSDVVNEYVRARSTGELAEASNDFGLLQFRRVMR"
     gene            9237..9638
                     /locus_tag="PKV56_11045"
     CDS             9237..9638
                     /locus_tag="PKV56_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX68394.1"
                     /translation="MSIRRVFDISEHLQPSDDEPIRSIVQETPDATIVAWIVRPGQCI
                     APHVHPHGQDTWTLLSGEGHYQTDEAGTTVRIATGDVVVASRGEVHGVRNTGSQPLVF
                     ISVVCPLEAGYRLLPSMAGTAPPLIGSSGQA"
     gene            complement(9705..9902)
                     /locus_tag="PKV56_11050"
     CDS             complement(9705..9902)
                     /locus_tag="PKV56_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOX68395.1"
                     /translation="MNDELDCKAISRLISDGQDRDLPPAERARMRLHFVICETCRDVN
                     DQFSFLREAMKRLSRNEPPAE"
     gene            complement(9899..10456)
                     /locus_tag="PKV56_11055"
     CDS             complement(9899..10456)
                     /locus_tag="PKV56_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX68396.1"
                     /translation="MSIESELAALHQPLLRFAQQQLRNDSLAEDVVSETFLAILEKPD
                     NFEGRSSLRTYATGILKFKIIDAIRHKGREVHIEPLDEQSMDDAIDALFIADGHWQDP
                     PVPWQHPERALEQSQFFDTLQICVDRLPPKLGRIFMMREWLESDVDEICTELGITSNN
                     CGVMLYRARMQLRECLDISWFGAQK"
     gene            complement(10559..11188)
                     /locus_tag="PKV56_11060"
     CDS             complement(10559..11188)
                     /locus_tag="PKV56_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX68397.1"
                     /translation="MPAVRSMRPMLALLTSALLLCGIARAQDTPTDQDVVRRAIHVCA
                     SCHGDEGRSKTPAIPSLAGQQRDYVIAQLKDFRSQTRAEAGTRAYMWGISALLDNPTI
                     FGLAEYYAALPPAPGQRGTPSLIAAGRKIYMDGIPDRAVRACASCHGDSAEGAAGFPR
                     LAGQHATYLHAQLKLFGSKLRPHGIVMKVETSFLKPTEMRAVAEYLQSL"
     gene            complement(11322..12101)
                     /locus_tag="PKV56_11065"
     CDS             complement(11322..12101)
                     /locus_tag="PKV56_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOX68398.1"
                     /translation="MRTTFAFTRYSMLSGWRRLEWIALVATIPAFYMAMLSVHRPITM
                     LLYGLACCACAVVIWTEAHQAWRQESRNGIYARHQLGLLLASALLLSAVLPAGEHFDV
                     LGVRLATALLLLLLRLGESLQPRLWRDGLPFVLTLAIGVVGLCGVGFWVLEPKVETFG
                     DGLWLAFTTAATVGYGDIVPSTPAAKIFAVFVVLLGFAVLSTVTASIAAVWVQSEERR
                     IEREILSDLHQQLKTIRDELAELRAELAERTEPHHGTSDSN"
     gene            12374..15439
                     /locus_tag="PKV56_11070"
     CDS             12374..15439
                     /locus_tag="PKV56_11070"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX68399.1"
                     /translation="MTHVTAEPVLTGDPLLSDFPVGDDARPQGWLPESRLASALLAGF
                     LYFALAVVSIALSRHGSTVAMLWYANAAGMAVLYSRPMRQWPVLLGAMALGSLAAGRV
                     FGDSAQFLLICISANTFEVLLGGLLLRRYCAPARCVSRAGALLKALLLGGVVPAFFGA
                     LLGAGLLSHYQLGPFERVWLSWFEGDSIGSVAVLPLGLLLAARGWQPVGAVLRRPAVL
                     MAWALVVALALWAPPSVPYPFVYISIGLVLVASLGRFAGASLGVLLCSLSLGVVIASG
                     TFEPMIATRPVDEGLLYLPLLLVLVPPLLLAANLERIQQGVLELARREDDFRTLYQEA
                     PVMMHSTDPQRRLISVNDEWLARLGYERAEVLGRPSMDFLTTESRKRAEESLIPRFIE
                     QGFLRDVDYQMVTKGGDIMDVQLSAIWQRDAQGRLLRTLSVMKETTEQKRLAAELAAE
                     KELIEVTLHSIGDGVVTTDPQARIIYMNPVAEQLTGCDLPAARGRAFGEVVQLYDEGS
                     DAVLPSPIQNCLAGGRVAGLPQSALLRDRHGREYGVQISAAPIFSKDRSLIGAVTVFQ
                     DVTEARALSQRLAHLAHHDSLTDLPNRVLFQDRVHQACQSGMRHRARFAVVFMDLDHF
                     KHVNDSLGHAVGDEFLRAVARRLTATLRGSDSVCRLGGDEFVMLVSDVAGPDDVAEVA
                     RKILREVAAPCSLQGTEVSVGISLGIAVFPDDGEDPATLMKHADAAMYRSKREGRNRY
                     QFFSRAVDLAASARLRLESDIRRGLAEGQFVAHFQPIIDVVTGRPVAVEALARWNRPE
                     HGLQGPLEFISVAEESGQIVALGAEMLRQACGQLRAWRGSVLDRITIAVNVSPVQLAE
                     AGFVDMVSETLRSTGVDGPQLAFEITESTLMHDPEAVLGVLRRIKGLGIRIAVDDFGT
                     GYSSLSYLKRFPVDSVKIDRSFVRDLETDPDDRELARAIVAMSRSLRLRIVAEGVETP
                     TQASMLGAMGCTSLQGFLYARPADAQTVSAWLHAKLVDAEPVELVKHTI"
     gene            15518..16228
                     /locus_tag="PKV56_11075"
     CDS             15518..16228
                     /locus_tag="PKV56_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68400.1"
                     /translation="MNIKTIRAAVMLLGVVASQAWAGRPLGTDDAATADLGTCLLEAW
                     NERTGSDRALIAGFACGVAKGIELGVDYTHPHPRDEVRGESGLALKWVPESWSHETAA
                     GKWDLGLKFSTAFEHPAHRSWRHAEASALVLSTLTPSDDWTIHANLGVAHSRDAHRTG
                     GILGVAAVWTPAEPLLLFTEVLANTRSQVFGGTVKTAGARWWLIQDVLGLDLTASRES
                     GSSATLWSVGFGWYGISF"
     gene            16252..>17661
                     /locus_tag="PKV56_11080"
     CDS             16252..>17661
                     /locus_tag="PKV56_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX68401.1"
                     /translation="MKLRTQILVLGLFGALLAAVSGGIGFVGVSRLGGSIDGAIQAGS
                     ALQTSLSADMMHDAVRGDAQLAYLGALDKNPQQIAEAKAGLADHAKTFDDAMAELAKL
                     QLSDESRAALNAVHPVIRKYIDTATAVITAAAVDPSQAKAQLPAFQAAFSELEDSMAA
                     LSESIEKNGTGVIDGATADVQQTKAFVVVSLLVGAALIVAAALALARNMKRPMEHAVE
                     VADQLAHGDLTSEIQPSGNDETVRLLESMAAMQESFSRIVRNVKANAETIATASTEIA
                     SGNHDFSNRTEQQASSLQQTAATMEQLGSTVRTNADNARQANQLAEGASTVAVKGGEV
                     MGQVVATISGINESSKKIAEIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAG
                     EVRSLAHRSAEAAREVRNLVHASVERVEAGTVLVDEAGKTMQEIVSSIRRVTDIVAEI
                     SAATTEQSTGISQVGQAVSG"
BASE COUNT         2868 a         5883 c         5964 g         2946 t
ORIGIN      
        1 aagtgcgttg tcggtcaagg cggggctgcc gacttgcagg gcgccccgac gtgcttcggc
       61 gtgcgctgca tcggcctcgc gcaggcaggt ggcccgatcc tggttcgagc gcccgctgat
      121 gcaggtcgcc cgctcctggc ggtggagcgc atcgaccgcc gtggcggctg cgggggccgc
      181 cccagcgcag tggcaggcga gcaccaattc gaggatcagc gcagcccgga tcgggcgcga
      241 agtcagctgg ggcagggtgc gcatggacaa ttcctccggt tcgacgagat ggcctcgact
      301 cgctccgatg ctgcggtcac cgcgccatct gcgtctgttc ggaggcagac cgaacaggtg
      361 cctccccgcg ggcacaatct gccgccatgc gcctaccgga agacatcgcg tcgaagctcc
      421 tcggcgtccc ctacgcctcg ctcgacgagc gaacgaagaa ggtcacgcgg tccgtagccg
      481 ggcgcacgca catcgcccgc aacctgacgc gcaccgacga tgagacgccc agcctcggac
      541 agcgggcggc cgatgcggtg gcctcgttcg ggggctcctg gacgttcgtc gggctgttcg
      601 cgctgacgat gctggtgtgg gtgggcatca acgccctgct gctgatctac cgcggcgcga
      661 cgttcgaccc ttacccgtac atcctgctca acctgttcct gtcgatgctg gctgcgatcc
      721 aggcgccgat catcctgatg tcgcagaacc ggcaggccga gaaggaccgg gccaccgtcg
      781 agcacgacta cgaggtcaac ctgaaggccg agctcgagat catgctgctg cacgagaaga
      841 tcgatctgtt gcgcgagacg cagtggaagg aactgctcga catccagcag gagcagctgc
      901 gattgctttc ggaacaggtc ggccgcaagg caccgggggc ctgatcgggc acggccctca
      961 gggcaccacg gtcaggaaca ggaacgacgc gaacagcacg aggtgcaccg cgccctgcat
     1021 cacggtggcg cggccgctgc ccagggtcaa ggtgcccacc accagcgtca gcatcagcag
     1081 cacgatgtcg atcggcctca ggccgaggac cagcgggaac gggaaccaag gcgacagcgc
     1141 cgcgaccacc gggatggtca gcccgatgct cgccaggccc gatccgagcg ccaggttcag
     1201 gctgctctgc atgcggttgc cgcgcgcggc acgcagcgcc gcgccggttt cgggaagcag
     1261 cacgatcagc gcgatcagca cacccgatac cgccttgggg gcaccagcgg cggccacgcc
     1321 ggcttccagc gcaggcgcca gcgccttggc cagccccacg accccgacca gcgacaccag
     1381 cagcaggccc aggctggtcc aggcgatcca gtgggcggga ggtggcgcgt gcgctttcgt
     1441 atcgtcggtt ccgaccggca ggaagtagtc gcggtggcgc accgtctgca cgaagacgaa
     1501 cacgctgtag agcgccagcg aggtgaatcc ggcgaacgcg agttgcgaac cgctgtaggt
     1561 gggcccggag gtcgtggtgg tgaagctcgg cagcaccagc gccagacctg ccagggcgcc
     1621 gagcgaggcg agcgccgggc tggtgccttc gacacggaag accagcacgc cgtgacgcag
     1681 cccgccgacg accaggcaca ggccgatcac gccgttgcac acgatcatca cggccgagaa
     1741 caccgtgtcg cgcgccagcg tgttggcgct ggcgccgccc gccagcatca gcgagacgat
     1801 caaggccacc tcgatgatcg tcacggccac cgccagaacc agcgtgccgt agggctcgcc
     1861 gacccggtgg gcgaccacct cggcgtggtg gatcgcgcag agcaccgcca gcaacagcgc
     1921 cgcggccagc agcgcgccga gaccgatgcc gagcggctgc ccccaggtcg acgccagcgt
     1981 cagcagggcc agcaccggga ccgcgatgct ccacaggggc aggccgggca ccggccagcg
     2041 ggcggcaggg ggatcggcat cggcacgggg gttcatcggt cgatcctaac gaacgctgcg
     2101 gccgggtcga gtcggtgcgc gcggacgggc tcctagaatc ggcgcatgcg ttcggcttcg
     2161 tcgacccgaa gaatccgtgt ctgggtggcc agcctggcca tcctgctggg tgcgctcgcg
     2221 ccgtcgatca cgcatgcgct gaacagcctg tctggcagcg ccttcctcga ggtctgcacg
     2281 gccgtcggct cccgtttcgg ctggagcgtc gacaacgagg ccagtgcgcc gaagagcggg
     2341 gtgcctgtgg atcaggcgca ccactgtccc ttctgcgtgc tgcatgcgtc ggcgcccggc
     2401 atgccgcctg cgccgttgca gggcctgacc ctgctgcccc tcgcgttcca cgtccccgag
     2461 ctctttctcg cggcgccacg cacgctgaac gcctggacaa cagtccaggc gcgagccccg
     2521 cctcaccact cctgacagcc cgggttcgtc ccgctccgcg ttgaaacgct gagcccgagg
     2581 cccgctcgcc cgccgtctcc cgacgccgga agtcaggaaa ttccacgaag tcgattcggt
     2641 cgggtcgcga ctgcgcgtgc cgtgtgcctg acgcacggcg ccgtccacgc ctgaacgacc
     2701 tttgcctgcg cgccgaggca ctcctcgcac gcgctccaca catcaagagc ctatccatga
     2761 agaagatttc cccacccctg ttcggcctgg ccctgctgca gatggccatc ggcgcggcat
     2821 acgcgcaagg cacgagcgcg ccgaagtccc tcgagaaagt cgtggtgacg gggtcgccca
     2881 tcatcgagag cagccgcatc gatgcgttcg gatcgctgac caccgaggtc ggcgccagcc
     2941 aggtgcggga cctgaacgca ctcgacctgt cctcggcgct gcgccgcacg cccggcgtca
     3001 tcgtgtcgcg cttcaatccg gtcggatcat tcggcggtga cgaaggcggc gcggtctacg
     3061 tgcgtggact cggggccagc cggccgggca gcgagatcaa gacctatgtc gacggcattc
     3121 cgttctacat gggtgtctgg aatcactcgc tgctcgacct gcttcccgtg cacggcatgg
     3181 agagcatcag cgtgcacaag ggtccgcaac cccagcgctt cggcaacacc tttgccgcca
     3241 tcgacctgat cccgaagcga gccgtcggcg acgggttcgg tggcgatgtc agggtgagcg
     3301 cgggcagcga ctcgacggcc gtggaacagg ccgcgctgac cttccggtcg gacgccaccg
     3361 agctcagcct ggctcagggt ttcgcgcgtt cggacggcca ccgcaaggat gccgatggcc
     3421 ggctcgccaa cctgatgctt cgaggcagcc atgacctgaa cgcgcaatgg cggatcggcg
     3481 gaatgatcct gggctcggac aacaaggtgc gcgaccccgg cgagaagggc cggccggcca
     3541 cgaagaccgg cagctatgtc acccgcggca cgctgggctc gctgtcgctg gagcatcgcc
     3601 acgatcaact ccagggccgg ctgcaggtct acgacaacga cggcatgagc aactggcaga
     3661 acccgaccgc cgccaacgtg cgcagccgct tccatctctc ggggctgcgc tggcgcgagg
     3721 agctggcgcc gtggaaggac ggcaccgtcg tcgcggggct ggatgtcgat cgcatgagcg
     3781 gcagcgtggc cttcaatgga ttcacggcct tcgacgacgt gtccctgcga ctcacgtccc
     3841 cgtacatcgg actggcccag cgcttcgagc tcggcgaccg ctgggccgtc acgccatccg
     3901 ccggcgtgcg tggctaccgg cacaacgtgt acggcagcag cagcgccccc cacgccgggg
     3961 tggtgatgga gaacgacacg gtggccgtgc ggctcaacgc atcccgcggc ctgaaccatc
     4021 ccggactgga cgcacctttg ctcaatgcga tcgtgccgcc gctgggcggc gcacccacca
     4081 gttggcgcag tctcgatccg gagcgcatgg accacgtgga ggccggcgtg cactggaccc
     4141 ctcgacgcgg cagcagcatc gatgtctccg tgttccacga cgagttgaag aaccggtacg
     4201 tgttcgcgtt tccgccagcg gtcgcgatgc ccagcttcac caacctcgga aactaccggg
     4261 ttcgcggact cgagggcgtg tggcagcagc agtggaccag cgcctggagc agcttcgcag
     4321 gcctgacgct gctggacagt tcgctcgatg atctgcccta tgcacccaag cgcgcgctgt
     4381 cgctcggcct gacctggcag gacggaccct ggcgcgtgag cggcgacgcg cagtcgcaga
     4441 gtcggatgtt cgtgctgaac aaggcccggg ccgatggcgc gcccaacacg gcgcatgtcg
     4501 gcggcttcgc ggtgctcaac ctgcgggctg cctatgccct gtcggccctc ggaccgcgcg
     4561 gcgaggtctt catcgcactg gagaatctgg gagatcgtcg ctacgcctac cgtcccggct
     4621 atccgatgcc cggcctcacc ggccagatcg gcctgaacta cagcctttga agcaggagcg
     4681 ctccacatga aatcattgat ttccacgacc ctgatgcttc tgcagctggc ctgtgtcatc
     4741 ccgtcggctc gtgctgcgga ctcaccggat gaagccggca tccggcaggt gctgatgacc
     4801 accttcgaca agcccgaggc ccggatgcag gtcgagccga tcgtcgtgca ggccgagcat
     4861 gccgtcgcgg gatggatcca gggcgagcgc ggcggccgcg cgctgatgcg cagggaaggc
     4921 agggactgga gcatcgtgct gtgcgggggc gacgggttga aggatccgca ggcgctggcc
     4981 atggccggca tcgagcccgc ggcggcacgg gccctggtgc agcgcctgaa cgcggcggag
     5041 gccaacctga cacccgcgaa gcgcgcccgc tttgccagct tcgaagggat ggttcacatg
     5101 gatgcccagg ggcggcaccc ggctccgggt caccactgac gaggaacggc gcacggccgg
     5161 ccaccggacg cgtgccgttg cgtggtgcag cccaggcacg gcgtcgcccg gatcggctac
     5221 aacacggggc tgccttcgtg caggttcaac gcaagacaac ggagttcgca tgagcatgaa
     5281 catggaatcc attcagacct tcctgggcac caccgccatc gatgtcggcc tcaaggtgct
     5341 ggcggccatt gcgttctggg tggtcggccg ctggatgatc ggtcgggtga tctcgctgat
     5401 gcaggcggcg atgaaccgca accacgtcga tccgacgctg acgaagtacc tcggatcgat
     5461 cattgcgatc gctctgaaca tcgcgctggt gctcggcatc ctcggttact tcgggatcga
     5521 gaccacctcg ttcgccgcgc tgctggccgg tgcaggcgtg gccatcgggg cggcctggag
     5581 cgggttgctg ggcaacttcg cggccggcgc gttcatgctg atcctgcggc cgttcaaggt
     5641 cggcgatttc gtcagcatcg cgggcatcgt cggcacggtc cacgaactgg gactgttcgg
     5701 caccaccctc gtgacgccgg acaacgtgat gaccatcgtc ggcaacggca agattttcgg
     5761 cgacacgatc cagaacttct cggtgctgcc ggtgcgtcgt gtcgaaaggg tggcgcaact
     5821 ggccaacggg gtcgatccgc tggatgcgat cgcgcgcttc aaggccgcgg tggcgctgat
     5881 cccgaacatc tcgaaggaga tgccgcccga ggtcaacctc ctcgatttca agctcgaagg
     5941 cccgcagatc gcagtgcggc cgtacaccca caccgatcac tactggcagg tctacttcga
     6001 caccaacgag gcgatcgtca aggtgtgcaa ggaggcgggc tggcccgtgc cggcggcgct
     6061 gcaccaggtt cgccagctgt gaatcgtgtg aggcggctga ccggcatcgc cggcagcgcg
     6121 cgttttaccg tggtcagcct gtccattcca cgatgagtct cgcgcgactg ttcctggcac
     6181 tcctgctgtt gttgatcggc ggggcttcgt gggctcaggc cgcatccgat cccccggccg
     6241 gcccggtgcc cgtcgccgca cagactgcgg tcgccgccga ggcagaggtg gtggtcatca
     6301 atcgcaccgt cacggtcttt cgcgcgcctt atctcggggt tgcacccgaa cgccgcgccc
     6361 ggcgcaccga ggcgatcctg agcgatctgc tcgaccggag cgtcctgggt gcagtgacca
     6421 tccaggtcga gccgcagggc catgtgctga tggtggatgg cgaactggcg ctggtgctga
     6481 cgccggccga cgtcgatccc ctgcgcgcac agacgctgga gcaggcgagt gcggccgcgc
     6541 gcgatgcgtt gacgcaggta ctgtccgaga cccgcgaggg tcgtgatgcc cggcggctgc
     6601 tgcatggcgc ggggcgcagc ctgattgcga ccctggtggt ggctgtcgtc gcgtggctgg
     6661 cctggcgtgc caggcgctgg accacggtgc ggcttgcgca gcgggtcgcg cgcagcgcgg
     6721 cagcggtgcg tgtggcaggc accgagttgc tgcatgccga acgcctggtg gctttcgtcc
     6781 gcctgggtgt ggccgcactc tggtggctgc tggcgttggc gctgtcctac gaatggctga
     6841 gctatgtcct gcgccagttc ccctacaccc ggccgtgggg cgagggcctg accgagtacc
     6901 tgttcggagt ggcgtcccag ctcggacgcg gcgtgctggg cgcgctgcct gatctggtga
     6961 tcgcggtggt catcttcctg atggcgcgcg gcgtgatcgc gatgtttcgc cccttcttca
     7021 atcggctggc cgccaacgac aaccgccagc gcggctggct tgatgccgac acggccgggc
     7081 cgactcggcg gctgttcaac atcggcgtct gggtgttcgc cgcggtgatg gcctatccct
     7141 atctgccggg tgcgcagagc gaggccttca agggcgtgtc gctgctcatc ggcctgatgg
     7201 tcacgctggg cggatcgagc ctgttcggcc aggcagccag cggactcgtg ctgatgtact
     7261 cacgcacgct gcgcgtcggc gagtacgtgc gcatcaacga gaacgagggc acggtcaccg
     7321 agatgggtgt cttcgtcacc cgcgtgcgca cgggcctggg cgaggaactg acgtttccga
     7381 actcgctggt gctgggcagc gtcagcaaga actactcgcg ggcggtggaa gggcggggtt
     7441 atgtcgtcga cacgacggtg accatcggct acgacacgcc ctggcgtcag gtcgaggcct
     7501 tgctgatcga ggcggcgggc cgcacgccgg gcgtgctgcg cagcccggca ccacgcgtct
     7561 tccagaccgc gttgtcggac ttctatcccg aatacaggct ggtctgtcag gcggtcccga
     7621 gcgagccgcg accgcgtgcg gaagtgctca gcgccttgca cgcgagcatc caggacgtgt
     7681 tcaacgaaca cggcgtgcag atcatgtcgc cgcactacct gggtgacccg gcagacccca
     7741 agctggtgcc gcctgatcgg tggtacgccg agccggcgcg tcgcagcgaa ccggcggccc
     7801 cgtcagcctg attcggctgc ggcgcggcac ccgctgccgg ccacttgcag gcacggcgcc
     7861 gtgcaggtgc cggtgcgctg tggacgaacg gccgagtgtg gggcagccgg tggtcagtgg
     7921 tcgcgtccgt ggccattgcc acggccgttt ccgtttccgc gatcgtcgtt gcgtcgatcg
     7981 ccccggcggt caccgtcgtg cctgtcccag ccgcgcttgc ggccgcgatc ccaccccggg
     8041 tgctcctgct ggtatcgctc gcgcacccac tggtcccgga cgaagtacac cggctggccg
     8101 caggcggcgt agcggccgca gtagcggcgc cagtcctgct gatgcaccgg cggcacgtaa
     8161 aggtagatcg gccgacgctc gtacatgacc ggcggcggag caatgatgac cggctgcgca
     8221 tagaccagcg ccgggcgcgg catgtcgccg atgtcgatgc ggccatagac gccgggctgg
     8281 cggatgctga tcgagacgcc gacgctggtt tcagcggatg cggatccgat ggccgccacg
     8341 gccagtacca ccgcgaagat tcttttcatt gggttgtctc cagaagatcc tgagtgaaca
     8401 acgctgatcg atcccccgac gccgacaggc gttcggtctg cagcctctgc tttacctgcg
     8461 gttacgcgcg aagtcggtgg gcagggttcg cagtcgcgat ggcttgttgc attgatcaca
     8521 aaaatgtgat gtgtccattc cagtcaaaag ccgtaatgaa ccggcccggc gcgtgactca
     8581 ttcaggacag caatgcaaaa cgcgctgcca ttcattccga ctttcaaccc actcttaagg
     8641 acccaccatg aacaagatca acgctctcct cctgacctcg ctttccctgt tcgccgccac
     8701 gacggcattc gcaggcgagt cggcctccac gcggacccgt gctgatgtca tcgccgaact
     8761 ggtggctgcc cgcgccgccg gcgagttgcc ggtctcgggc tacgaaacca gctacacggt
     8821 caacacccgc agcgccgctt caacgctcac acgcgctgat gtgcaggccg agtacgtgcg
     8881 ggccaggcag gccggcgaac tggaagaggt caccggctac tcgagcgtgg cgaacgtcgc
     8941 gacgagccct tcgcggctga cccgctccga cgtcgtcaac gagtacgtgc gtgcccgcag
     9001 caccggcgaa ctggccgagg ccagcaatga cttcggcttg ctgcaattcc gccgcgtgat
     9061 gcgctgaagc ccgaaccggg cctggtgaaa gctcaccggg ctgcggtaca ggcattcaag
     9121 cggacgcgac tgcgctggcg tgtcgaccac tgttccgggt gtcccggcac acggtgcacg
     9181 ctgcgcagcg gtccggcacg acacttgcca ggcttggcca tcacctgcga ggtcccatga
     9241 gcattcgccg cgttttcgac atctccgaac acctgcagcc ctcggacgac gagccgatcc
     9301 gatcgatcgt gcaggagacg cccgatgcca ccatcgtggc gtggatcgtg cgccccggcc
     9361 agtgcattgc gccccacgtg catccgcacg gccaggacac ctggacgctg ctctcgggcg
     9421 agggccacta ccagaccgac gaagcaggca ccaccgtgcg aatcgcgacc ggagacgtcg
     9481 tcgtcgcgtc gcgaggcgag gtgcacggcg tgcgcaacac cggctcgcag ccgctggtct
     9541 tcatctcggt ggtttgcccg ctcgaggccg gctacaggct gctgccgtcg atggcgggga
     9601 ccgccccgcc actgatcggg tcttccggtc aggcgtgatt gcggcgtccc agaaacttcg
     9661 caggcctcgc ccgcagtcgc agggcagcct ctgcgcgccc cgtctcactc ggccggcggt
     9721 tcgttccggc tgaggcgctt catggcctcg cgcaagaagc tgaattgatc attgacatcg
     9781 cgacaggtct cgcagatcac gaagtgaagg cgcatgcggg cccgctccgc cggcggcagg
     9841 tcgcggtcct gtccgtcgct gatcagacgc gagatcgcct tgcagtcgag ttcgtcgttc
     9901 atttctgtgc accgaaccag ctgatgtcca ggcattcacg cagctgcatg cgtgcccgat
     9961 agagcatcac gccgcagttg ttgctggtga tcccgagctc ggtgcagatt tcatcgacat
    10021 cgctttccag ccattcacgc atcatgaaga tgcgcccgag cttcggtggc aggcggtcga
    10081 cgcagatctg cagggtgtcg aaaaactggc tttgttcaag tgctcgctcg gggtgttgcc
    10141 agggaaccgg tgggtcctgc cagtgaccgt cggcgatgaa gagcgcatcg atcgcgtcgt
    10201 ccatgctctg ctcgtcgagg ggttcgatat gaacctcgcg gcccttgtgg cggatcgcat
    10261 cgatgatctt gaacttcagg attccggtcg cataggttcg caaggagctg cggccttcga
    10321 agttgtccgg cttctcgagg atggccagga aggtctcgga gaccacgtcc tcggccaggc
    10381 tgtcgttgcg aagctgctgc tgggcaaacc gaagcagtgg ctggtgcagg gcggcgagtt
    10441 cggattcgat gctcatgaca gggaaaaggc cattctgaaa tggagatgtc ggacgccgat
    10501 gacactccat tacaacttat gtggccgatg ccccttcatg gagcccccgc tcagaggctc
    10561 aaagcgactg cagatactcc gcgaccgcac gcatttcggt gggcttgagg aacgaggtct
    10621 cgaccttcat gacgatcccg tgcgggcgca gcttgctgcc gaacagcttg agctgcgcgt
    10681 gcaggtaggt cgcgtgctgc ccggccagcc tcgggaatcc ggctgcgccc tcggcggaat
    10741 cgccatggca cgaggcacag gcgcgcaccg cacgatcggg gatgccgtcc atgtagatct
    10801 tgcgaccggc tgcgatcagc gacggggttc cgcgctggcc gggggcgggc ggcagcgcgg
    10861 cgtagtactc ggccagtccg aagatcgtgg ggttgtcgag cagcgccgag atgccccaca
    10921 tgtaggcgcg cgtgccggct tctgcacgcg tctgcgagcg gaagtccttc agctgcgcaa
    10981 tgacgtagtc gcgctgctgg cccgccagcg atggaatcgc cggcgtcttg ctgcggcctt
    11041 cgtctccatg gcacgaagcg cagacgtgga tcgctcgacg caccacatcc tgatcggtgg
    11101 gcgtgtcctg tgcgcgggca attccgcaca gcaacagcgc ggatgtgagc agggcgagca
    11161 tcggtctcat cgatcggacg gctggcatga gtcttctccc gtgggttggg tggatctgtg
    11221 cttcattgtc gaaaccgcct gccgcgccag gattgatgca acgcaatgat ccggtcgatc
    11281 ccacgctgct tccggctgaa cccgctcgcg gtgcactccg atcagttcga gtcgctcgtt
    11341 ccatggtgcg gctcggtgcg ctcggccagc tcggcacgca gttcggccag ctcgtcccgg
    11401 atcgtcttca gttgctggtg cagatcgctg agtatttcgc gctcgatgcg ccgttcttcc
    11461 gactgcaccc acacggcggc gatggacgcg gtgacggtgg acagcaccgc gaaccccagc
    11521 agcaccacga aaaccgcgaa gatcttggcg gccggggtgc tgggcacgat gtcaccgtag
    11581 ccgaccgtcg ccgcggtggt gaacgccagc cacagtccgt cgccaaaggt ttctaccttg
    11641 ggctccagca cccagaatcc gaccccgcac aggccgacca cgccgatggc cagtgtcagc
    11701 acgaagggca ggccgtcgcg ccagagccgc ggctgcaggg attcgcccag ccgcagaagc
    11761 agcagcagca aggctgttgc caggcgcacg ccgaggacgt cgaagtgttc ccctgccggc
    11821 aggacggccg aaagcagcag ggcactggcc agcaacaggc ccagttgatg ccgggcatag
    11881 atgccgtttc gagattcctg ccgccatgcc tggtgggcct ccgtccagat cacgaccgcg
    11941 caggcgcagc aggccagccc gtaaagcagc atcgtgatcg gccgatgcac ggacagcatc
    12001 gccatgtaga aggccggaat ggtcgcgacc agcgcgatcc attcaaggcg ccgccagccg
    12061 ctcagcatcg agtagcgggt gaaggcgaat gtcgtgcgca tccgctagcg tatccagcgc
    12121 gcgtgacggc ctcttgatgc gggtcaatcg ggcatccctt gcagtgcggg catcggcgga
    12181 cgatcgtcgt tgacagcctt gcgcgaggag aggccctgtt cggccctctt ttcagtcgtt
    12241 tcggccggct cgggccctcc agaatcagct tccatcgcca cggcgagtcg tgcacggctt
    12301 gttgccgctc tcgctccggg ggctgcctgc cagcctccgg tggaatcgtc tcgacgaaga
    12361 aggtatgcgt tcgatgaccc acgtgacagc cgagcccgta ttgacgggcg atcctcttct
    12421 ttccgacttc ccggtcggcg acgacgcgcg tccgcagggc tggctgccgg agtcccggct
    12481 ggcttcggcg ctgctggccg gcttcctcta cttcgccttg gccgtggtct ccatcgcgct
    12541 gtcgcgccac ggcagcaccg tggccatgct ctggtatgcc aacgccgcag ggatggccgt
    12601 gctgtactcc aggcccatgc ggcaatggcc ggtcctgctc ggcgcgatgg cgctgggcag
    12661 cctggctgcg ggccgggtct tcggcgactc tgcgcaattc ctgctgatct gcatttcggc
    12721 caacaccttc gaggtcttgc tgggcggact gctgctgcgc cgctactgcg ccccggcaag
    12781 gtgcgtcagc cgagcaggag ccttgctcaa ggcgttgctg ctcggcggcg tggtgccggc
    12841 tttcttcgga gcgctgctgg gtgccgggtt gctgtcccac taccagctcg ggcctttcga
    12901 acgggtgtgg ctgtcgtggt tcgagggcga ctcgatcggc agcgtcgcgg tgctcccgct
    12961 gggtctgctg ctggccgcgc gagggtggca gcccgtcggt gccgtgctgc ggcggcctgc
    13021 ggtcctgatg gcctgggcgc tggtggtggc gctcgcgttg tgggcgccgc cttcggttcc
    13081 gtaccccttc gtgtacatct cgatcggtct ggtgctggtg gcgtcgctcg gccgtttcgc
    13141 gggtgcctcg ctgggcgtcc tgctgtgttc gctctcgctg ggcgtcgtga tcgcctccgg
    13201 caccttcgag ccgatgatcg ccacgcgccc tgtcgacgag ggcctgctct acctgccgct
    13261 gctgctggtg ctggtgccgc cgctgttgct ggctgccaat ctcgaacgca tccagcaggg
    13321 cgtgctcgaa ctcgcgcgac gcgaggacga cttccgcacc ctgtaccagg aggcgccggt
    13381 gatgatgcat tcgaccgatc cccagcggcg gctgatcagc gtcaacgacg aatggctggc
    13441 ccgcctcgga tacgaacgag ccgaagtcct gggccgtccg tccatggatt tcctgacgac
    13501 cgagtcccgc aagcgtgccg aggagtcact catcccgcgc ttcatcgagc agggcttcct
    13561 gcgcgacgtc gactaccaga tggtgaccaa gggcggcgac atcatggatg tccagctgtc
    13621 cgcgatctgg cagcgcgacg cgcaggggcg gctgctgcgc acgttgtcgg tgatgaagga
    13681 gacgaccgag cagaagcgcc tggccgccga actcgcggcc gagaaggaac tgatcgaagt
    13741 cacgctgcat tcgatcggcg atggcgtggt gaccaccgac ccgcaggctc gcatcatcta
    13801 catgaacccg gtggccgaac aactgaccgg ttgcgacctt ccagcggccc ggggccgtgc
    13861 tttcggcgag gtggtccagc tgtatgacga gggcagcgat gcagtgctgc cgagcccgat
    13921 ccagaactgc cttgccggcg gtcgagtcgc cggccttccg cagagcgcgc tgctgcgcga
    13981 tcggcacggc agggagtacg gcgtccagat ctccgccgcc ccgatcttca gcaaggaccg
    14041 ctcgctgatc ggcgcggtga cggtattcca ggacgtgacc gaagcgcgtg cgctttcgca
    14101 gaggctggcc catctggccc atcacgacag cctgacggac ctgcccaacc gggtgctgtt
    14161 ccaggaccgc gtccatcagg cctgccagtc cggaatgcgt caccgcgcgc gatttgccgt
    14221 cgtcttcatg gacctggatc acttcaagca cgtcaacgac tcgctcgggc atgcggtcgg
    14281 cgacgagttc ctgcgagccg tcgcgcggcg cctcaccgcg acgttgcgag gcagcgactc
    14341 cgtctgccgc ctcgggggcg atgaattcgt catgctggtg tccgacgtcg ccgggcccga
    14401 cgatgtcgcc gaagtggcca gaaagatcct gcgcgaggtg gctgcgccgt gctcgctgca
    14461 gggcaccgag gtcagtgtcg gcatcagcct gggtatcgcc gtgttcccgg acgacggcga
    14521 agaccccgca acgctgatga agcacgccga cgcggcgatg taccgctcca agcgcgaagg
    14581 ccgcaaccgc tatcagttct tctccagggc ggtcgatctg gccgcttcgg cacggctgcg
    14641 cctcgagtcc gacatccgtc gcgggctggc cgaagggcag ttcgtcgcgc acttccagcc
    14701 catcatcgat gtggtgacgg gccggccggt cgccgtggaa gccctcgcgc gctggaaccg
    14761 gcccgaacac gggctgcagg ggccgcttga attcatcagc gtggccgagg agagcgggca
    14821 gatcgtggca ctgggtgcgg aaatgctgcg gcaggcctgc gggcaactgc gcgcctggcg
    14881 cggcagcgtg ctcgaccgga tcacgatcgc ggtgaacgtc tcgcccgtgc agctggccga
    14941 agccggtttc gtcgacatgg tgtccgagac gctcaggtcc actggggtgg acggcccgca
    15001 gctggccttc gagatcacag aatcgacgct gatgcacgat cccgaggccg tgctgggcgt
    15061 actgcggcgg atcaagggac tgggcatccg catcgcggtc gatgacttcg gcacgggcta
    15121 ttccagcctg agttacctga aacggtttcc ggtcgacagc gtcaagatcg atcgatcctt
    15181 cgtgcgtgat ctcgaaaccg acccggacga ccgcgaactc gcccgggcga tcgtcgcgat
    15241 gtccagaagc ctgcggctgc gcatcgtggc cgaaggcgtc gagaccccga cgcaggccag
    15301 catgctcggt gcgatgggct gcacctcgct gcagggattc ctctacgcga ggcccgccga
    15361 tgcccagacc gtcagcgcct ggctgcacgc aaagctggtg gatgccgagc cggtcgaact
    15421 ggtcaaacac acaatttgat ccgggcagtc gcgcggcgac tcaagtattt gcatcgtgat
    15481 gtcgataccg gtcccatcag ccataggagt aacccctgtg aatatcaaga ccatccgcgc
    15541 tgccgtgatg cttctgggcg ttgtcgcgag tcaggcctgg gccggcagac cgctcgggac
    15601 cgatgatgcg gccacggccg atctgggcac ctgcctgctc gaggcctgga acgaacgaac
    15661 cggaagcgat cgggccctga tcgcaggctt tgcctgcggc gttgccaagg gcatcgagct
    15721 cggggtcgac tacacgcacc cgcatccccg cgacgaggtt cgcggcgagt ccgggctggc
    15781 gctgaaatgg gttcccgagt cgtggtcgca tgaaaccgcc gccggcaagt gggatctggg
    15841 gctgaagttc tccaccgcct tcgagcatcc ggcgcaccgc agctggcgcc acgcggaggc
    15901 ctccgcgctg gtgctgtcga cgctgacccc ctcggacgac tggacgattc acgccaacct
    15961 cggcgtggct cacagccgcg atgcgcaccg cacgggcggc atcctcggcg tggccgcagt
    16021 ctggacgccg gcggaaccgc tgctgctgtt caccgaggtg ctggccaaca cccgctcgca
    16081 ggtcttcggt ggcacggtga agaccgccgg tgcccgctgg tggctgatcc aggacgtgct
    16141 cggtctcgat ctcaccgcca gccgcgaatc cggctcatcc gccaccttgt ggtccgtcgg
    16201 cttcggctgg tacggcatct cgttctgatt tcacaggaag cagtacccac catgaaactt
    16261 cgaactcaaa tcctcgttct cggtctgttc ggtgcgctgc ttgcagcggt gtcgggaggc
    16321 atcggcttcg tgggtgtgtc gcgtctcggc ggctccatcg acggcgccat ccaggccggc
    16381 agtgcgctgc agaccagcct gagcgcagac atgatgcacg acgccgtccg cggcgatgcg
    16441 caactggcct acctcggcgc gctggacaag aacccgcagc agatcgccga ggcgaaagcg
    16501 ggcctggccg accacgccaa gaccttcgac gacgcgatgg cggaactggc caaactccag
    16561 ctcagcgacg agagccgcgc cgcactgaac gcggtccatc cggtgatccg aaagtacatc
    16621 gacacggcca cggccgtcat caccgctgcc gctgtcgacc ccagccaggc gaaggcccag
    16681 ctgccggcat tccaggcggc gttcagcgaa ctcgaggaca gcatggcggc gctgtccgaa
    16741 tcgatcgaga agaacggaac cggtgtcatc gacggcgcca ccgccgatgt gcagcagacc
    16801 aaggccttcg tcgtcgtttc cctgctcgtc ggcgcggcat tgatcgtcgc cgctgcactg
    16861 gccctggcgc gcaacatgaa gcgcccgatg gagcatgcgg tcgaggtcgc cgaccagctc
    16921 gctcatggcg acctgacctc cgagatccag cccagcggca acgacgagac cgtgcggctg
    16981 ctcgagtcga tggctgccat gcaggaaagc ttctcgcgga tcgtccgcaa cgtgaaggcc
    17041 aacgccgaaa ccattgccac ggccagcacg gaaatcgctt cgggcaacca cgatttctcc
    17101 aatcgcaccg agcagcaggc cagttcgctg cagcagacgg cggcgaccat ggagcaactg
    17161 ggctcgaccg ttcgaaccaa cgccgacaac gccaggcagg cgaaccagct tgcggaaggc
    17221 gcgtccaccg tggcggtcaa gggtggcgaa gtcatgggcc aggtggttgc gacgatctcc
    17281 ggcatcaacg aaagctcgaa gaagatcgcc gagatcatcg gcgtcatcga cggcatcgcc
    17341 ttccagacca acatcctggc gctcaacgcc gcggtcgagg cggcccgggc cggtgagcag
    17401 ggtcggggtt ttgcggtggt ggccggcgag gtgcgcagtc tcgcgcatcg cagcgccgag
    17461 gccgcacgcg aggtgcgcaa cctggttcac gccagcgtcg agcgggtcga agccggcacc
    17521 gtgctggtcg acgaggccgg caagaccatg caggagatcg tgagttcgat ccggcgtgtc
    17581 accgacatcg tggccgagat cagcgctgcg accacggaac agagcaccgg catcagccag
    17641 gtgggccagg cggtgtccgg c
//
LOCUS       DAOORV010000075        17605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3918_length_17605_cov_7.165584, whole genome shotgun sequence.
ACCESSION   DAOORV010000075 DAOORV010000000
VERSION     DAOORV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_17605_cov_7.165584"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKV56_11085"
     CDS             <1..377
                     /locus_tag="PKV56_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOX68402.1"
                     /translation="PILRNADQMSFADIEKKISEYGKKAAEGKLALEELTGGTFSISN
                     GGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGRE
                     AVLGLVAMKEALEDPARLLFDI"
     gene            399..1826
                     /gene="lpdA"
                     /locus_tag="PKV56_11090"
     CDS             399..1826
                     /gene="lpdA"
                     /locus_tag="PKV56_11090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOX68403.1"
                     /translation="MSKQFDVVVIGAGPGGYVAAIRAAQLGFSVACIDEWKNDKGGPA
                     PGGTCTNVGCIPSKALLQSSEYFEHAEHSFADHGIGLSNLSIDVRKMLGRKDAVVKQN
                     NDGILYLFKKNKVTFFHGRGAFVKAGDAGYDIKVSGAAEEVITGKHIVVATGSNARSL
                     PGAAFDEDKILSNDGALRISEVPETLGVIGSGVIGLEMGSVWRRLGAEVTVLEGLPTF
                     LGAIDQQIAKEAHKAFTKQGLMIELGVKIDKVTSGKSSVTITYTNAKGAACKLEVDKL
                     IVSIGRGPNTVGLNAEAVGLNLDERGAITVDAECRTNLPNVWAVGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHPHVDFNTVPWVIYTSPEIAWVGKNEQQLKAEGREFKAGTFPFLA
                     NGRARAMGDTTGMVKMIADAKTDEILGVHIVGPFASELIAEAVVAMEFKASAEDIARI
                     CHAHPSLSESTKEAALAVDKRTLNF"
     gene            1911..3008
                     /gene="zapE"
                     /locus_tag="PKV56_11095"
     CDS             1911..3008
                     /gene="zapE"
                     /locus_tag="PKV56_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOX68404.1"
                     /translation="MSVRQLYQQTLADRGYSADPAQLAAVASLERCENEWIEYKSRRR
                     NALTKLLIRPEIPRGVYMYGGVGRGKSFLMDCFFNSVPLERKARLHFHEFMREVHREL
                     ADLHGTVNPLEVLGQRMSRRFRLICFDEFHVADITDAMILYRLLESLFNHRVSIVTTS
                     NFHPDGLYPNGLHRERILPAIALLKDRLEIINVDAGTDYRRMTLEQVEMYQTPLGEAA
                     DAAMTAAFERLAETRDENPRLNIEHRVLKARRRAGGVVWFDFQELCGGPRSQNDYLEL
                     ASQFHTVLVSGVVQMSPRLASEARRFTWLVDVMYDRRVKLVLSAEVPAEDLYTEGPLA
                     HEFPRTVSRLHEMRSPEFLALARRDVETALT"
     gene            3062..3730
                     /locus_tag="PKV56_11100"
     CDS             3062..3730
                     /locus_tag="PKV56_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68405.1"
                     /translation="MALSPLAWAGDYSSEKARINKARDEADAILAAKEAECRQRFVVN
                     SCLIEARQQHRAVVQPLREELILLDEKQRKQRAADRLDRIRAKSDPASSRSDAAALPA
                     ASASVPSAAASRSSPRPLIRPRLRSFGPASESPLADMPAALPEPSSTPESVMEIPAAD
                     PGHARKRLTPMPNANALKDSTDRAREIEERRDRVLKRNAEKAARKPPAAPLPVPAAAS
                     SVAG"
     gene            complement(3747..4517)
                     /locus_tag="PKV56_11105"
     CDS             complement(3747..4517)
                     /locus_tag="PKV56_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOX68406.1"
                     /translation="MTSTAQGHRLSAATGIHRGDRAYQQDQVEIIPHARVPGCALGVL
                     ADGMGGKSGGRKAADQVILTAQQLFERYAPSRDDPAETLKQLVLEAHLMIKLTAITSE
                     EEPHSTVAAFLISPTRECHIVHAGDSRVYFFRGAEMVSRTSDHSFVQRLVNEGQLTEE
                     EANSHPQSNLLTGCLGTTQDPPLTVTRIERMERGDTVLACSDGLWHYFTPRELGAIVD
                     ALPPREASEMLIDKARQRARGGGDNLSLALVRVDALAA"
     gene            4578..4781
                     /locus_tag="PKV56_11110"
     CDS             4578..4781
                     /locus_tag="PKV56_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOX68407.1"
                     /translation="MNTNLHSLPRLLIELRIEHADLDGLIDQATLSLPEDELMLRRLK
                     KRRLALKDQISRLEAELDPPQPA"
     gene            4834..6801
                     /locus_tag="PKV56_11115"
     CDS             4834..6801
                     /locus_tag="PKV56_11115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOX68408.1"
                     /translation="MQAFSASGALSRADPAYREREVQLRMATAVADAIDTRGTLVAEA
                     GTGVGKTFAYLVPTLLSGARALISTATKSLQDQLFLRDLPRLRESLALPTTMALLKGR
                     ASYLCTHRMGLARHSTDPSDRWAARTLARIEAWSQGTATGDLAEIDGLDERSSAIPLV
                     TSTRENCLGSECPDYRSCHVMKARRDAMAADIVVVNHHLFFADMALRDSGVAELLPSV
                     EVAVFDEAHQLSEAGVAFLGVTLSSGQLLDTARDMLAVGLQQARGLAAWQDLAAACDR
                     SARELRLACVGVTRATEGRGGHAPRGSMKLRWDDRASRPAFGVALDQAGAACATAHAA
                     LDTVSELSPDFAKLADRMARLHELTERFAESAQPGQVRWIEVSANQCRLVESPLDIRD
                     ALREQMMSAPKAWIFTSATLGEDDRLSWFTEPAGLTDAATLRVGSPFDYAEHARLYVP
                     GQFPKPNDPDHPTCVTRLAARCARTLGGRTFVLTTTLRALQAIGDGLTADFEAGGDDI
                     SVLVQGQLPKRQLMQMFLANPRSVLIGSQTFWEGIDVPGDALQCVLIDKLPFPPPSDP
                     LVEARVKRLENEGRNAFTDYFVAEAAVSLKQGAGRLIRSETDRGLLVVCDPRMSSMGY
                     GKRLRLALPAMPRLESEDDAMGWLAGLASER"
     gene            complement(6835..7632)
                     /locus_tag="PKV56_11120"
     CDS             complement(6835..7632)
                     /locus_tag="PKV56_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOX68409.1"
                     /translation="MRPATLTGLGLALVWILAGCSSGPKDPTADWSPEKLYAEAREEQ
                     DAGNFDKAAKMYESLEGRGAGTILAQQAQLERSYLLYRSGEKAQALATVERFIKLHPT
                     SPAIDYAMYLQGLINFNDNLGFLGNLSRQNLSERDQQASRDAFQAFKQLVDQYPQSKY
                     SVEARQRMAYIVNALAEYEVHVARYYFRRGAYVAAANRAQQTIQEFQYSPSTEEALSI
                     LIRSYDRLGLDQLRDDAERVMRTNYPNSAFLSGEGVKSSTRPWWQLW"
     gene            7732..8751
                     /locus_tag="PKV56_11125"
     CDS             7732..8751
                     /locus_tag="PKV56_11125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX68410.1"
                     /translation="MPVPSAAPLEAGLAMDPDDLGEPANDEPVDVPVERRQAKVTSEL
                     HGSRLDKTLVSMAGEFSRNHLQSLIEAGHVWIDGRPQASASRKVLAGQVVTVELTPTQ
                     ECQAFRPEAMSLDIVHEDEHLMVVNKPAGMVVHPAAGNWSGTLLNGLLAHHAGAATLP
                     RAGIVHRLDKDTSGLMVVGKTLSAVTALVRQIAARDVRRIYLAICHGHFEHRHLRIEA
                     PIGRDPRSRVRMAVVSSGRSARTDVERLAVLDDESAPALSALRCSLHTGRTHQIRVHL
                     ASVGHPLLADATYGGRELFGMTRQALHATRLGFAHPASGEPLVFEAPPPPDLASVWHL
                     LTSVG"
     gene            8793..8915
                     /locus_tag="PKV56_11130"
     CDS             8793..8915
                     /locus_tag="PKV56_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68411.1"
                     /translation="MKSGLRVTDETAAAAPIADVRAFLDDPHGRFCRRDIAQPF"
     gene            9000..9704
                     /gene="scpB"
                     /locus_tag="PKV56_11135"
     CDS             9000..9704
                     /gene="scpB"
                     /locus_tag="PKV56_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOX68412.1"
                     /translation="MNTQDAKRVLQTALICAREPLAMREMRALFADELGTDTLHELLG
                     ELVREWEGEGVELVAVSTGWRFQSRPEMREFLDRLHPEKPPKYSRAVMETLAIIAYRQ
                     PVTRGDIEDIRGVTVATQIVKQLEDRGWVEAIGYREAPGRPALLATTRQFLDDLGLAS
                     LEQLPLIQGAAALPESLGELLPDQGVLLEQQESLDPDPETATPADAPLVTQALDLLPD
                     PAADAAPMERPNESKS"
     gene            9733..11541
                     /locus_tag="PKV56_11140"
     CDS             9733..11541
                     /locus_tag="PKV56_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX68413.1"
                     /translation="MPADVPGEGTEAPAKPPRRKRAAKTEASVSSGESISTEAVDAPA
                     EAPEASERPAVEGEEAVQVAGEAPTDSGAPDPTRRARGPRRRGPRPDDEGPSVTASAP
                     DDQADETDSTEPVFSLEPAVPPMQVGEVFAQVLSGEFDIEPPAEEAVAPPKRVLRPEP
                     EAPKLHKVLAQSGIGSRRDMEQAILDGQVEVNGQPAHTGQRISFGDQVKVNGKPVRIR
                     IIPPPPRIIAYHKPTGEVVTNDDPEHRPTVFRRLPRLPQGKWQSVGRLDMNTEGLLLF
                     TNSGELANRLMHPRFGIEREYAVRVLGTLDAESKAMLLEGVTIDGQMASFRSIENGGG
                     EGLNHWYRVVITEGRNREVRKLFEAVDLTVSRLIRIRYGCVVLPRGLRRGNWVDLEDS
                     DVRAIRRLAGGEAEGGPREARPNPRDGQQARPQRGRDKPPQGDRRNERPPRQGGDRPV
                     NEGRGPRPQQSESPLRAERVVEDRPPRDDDLDDDFDPSRIPNPLEQTFDKRFVQNPRT
                     PAGGRNFRTGGGGFGAGGSAPPAQKKGDGPRQPDPMQTSVGYIGGEAFHRKHARNGGG
                     QGGGGGGGRGGNRGGGYGGGGGGGGGNSGGGGRRGR"
     gene            11607..12032
                     /gene="ndk"
                     /locus_tag="PKV56_11145"
     CDS             11607..12032
                     /gene="ndk"
                     /locus_tag="PKV56_11145"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOX68414.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYSRFEGAGLKVIAARMAHLSR
                     GEAEAFYAVHKARPFFKDLVDFMISGPVMIQALEGENAIMKNRDLMGATDPKKAEKGT
                     IRADFADSIDANAVHGSDAAETAAVEIAFFFPGAQVYSR"
     gene            12077..13282
                     /gene="rlmN"
                     /locus_tag="PKV56_11150"
     CDS             12077..13282
                     /gene="rlmN"
                     /locus_tag="PKV56_11150"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOX68415.1"
                     /translation="MSAVNLLDFDLAGLTAYCEQLGEKRFRATQLFRWVHQRGMSDFA
                     RMSDLAKSFRDKLGAVAAVRVPAVLSEQASSDGTIKWLFDVGHGDAVEAVFIPEDDRG
                     TLCVSSQAGCAVGCRFCSTGHQGFSRNLSTSEIVGQLWFAEHHLRQRLHLPEGTRAIT
                     NVVMMGMGEPLQNYAALLPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLRDDCPVA
                     LAVSLHAPNDALRDDLVPLNKKYPLSELLAACERYLEAAPRDFITFEYCMLDGVNDRH
                     EHARQLVDLVQGRARDAQGRPIHAKPLSCKLNLIPFNPFPASGLIRSPRAQVLEFAGI
                     LQDAGIVTTIRKTRGDDIDAACGQLAGEVQDRTRIEQRRAGRAAIPILAVNTSASPVE
                     AIPINEGHA"
     gene            13279..14160
                     /gene="pilW"
                     /locus_tag="PKV56_11155"
     CDS             13279..14160
                     /gene="pilW"
                     /locus_tag="PKV56_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HOX68416.1"
                     /translation="MTIPAVSTDLRGWLWRCSAGLVLLGAWLLAGCAAPPPAAAAPGS
                     KEPVVNGPVTSTGTKDHITQSDETDASRRAQVRLELAGAYFGRGQMTTALDEVKKSIA
                     ADPNVSGAFNLRGLIYASLGDHRLAEESFRRALQIDPRDADSMQNFGWYLCQQSRFSE
                     ANVLFLQALAVPQYQDSPRTLLTQGICQARSGKNDEAERTLVRAYALEPGNPAIAVNL
                     SEVLYRRGDLERARFYIRRVNSVPNLVSPQTLWLAARIENKFGNPAGVADFGGQLRNR
                     FPESPESTAYVEGRFNE"
     gene            14225..15070
                     /locus_tag="PKV56_11160"
     CDS             14225..15070
                     /locus_tag="PKV56_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOX68417.1"
                     /translation="MLREARQAKGLHIAALAASIKVSQRKLELLEADRISELPDATFA
                     RALAQTVCRSLKIDPTPVLAALPQAPSYRLEGVDEGINTPFRDRPGRHEPTDFAAVAS
                     PAVWVPALIVVAALVVYLLPSEWVASMQSAVSGQPADQPSEPAPATPAIAVEPVQIEP
                     ATAASSPALVASDAAATTTSIGESASPLPGAAVVSEAAASSPAAGPLQVKAAQPSWVE
                     VVDARSQVLVSRMLQPGETVVLDGAMPFRLKVGNAAGTEVAFRGQAVDLAAATRDNVA
                     RLELK"
     gene            15080..16396
                     /gene="ispG"
                     /locus_tag="PKV56_11165"
                     /gene_synonym="gcpE"
     CDS             15080..16396
                     /gene="ispG"
                     /locus_tag="PKV56_11165"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOX68418.1"
                     /translation="MSDLRDVSGSATESFAALDDFIEPARPAPKRTRQAQVRWGSRVV
                     TIGGAAHVRVQSMTNTDTVDVIGTAIQVKELAVAGSELVRITVNTPEAAAAVPYIREQ
                     LDRMGIDVPLVGDFHYNGHRLLSEHPACAEALSKYRINPGNVGKGDKHDRQFAQMIEI
                     AAKNDKAVRIGVNWGSLDSELLTQMMDDNAKLADPHPPQQIMYRALITSAITSARRAE
                     ALGLNGDQIILSCKMSGVQDLVSVYRALAKRCDYALHLGLTEAGMGTKGTVASATALA
                     LLLQDGIGDTIRVSLTPQPGEARAQEVVVALEILQSLGLRSFNPSVTACPGCGRTTST
                     TFQELAKQIDDHLRAQMPVWRARYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTG
                     EAPAAPVFIDGEKAMTLRGDNIAGEFHALVESYIEKRYGAATLSDG"
     gene            16498..>17605
                     /gene="hisS"
                     /locus_tag="PKV56_11170"
     CDS             16498..>17605
                     /gene="hisS"
                     /locus_tag="PKV56_11170"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOX68419.1"
                     /translation="MSETIQAVKGMNDILPPESATWDWFEGRVRSLMARHGYAGVRTP
                     IVEPTALFVRGLGEVTDIVEKEMYSFVDSMNGDRLSLRPENTAGVVRAITEHSLLRDG
                     GKRLYYIGPMFRHERPQKGRYRQFHQVGAEALGYAGPDVDAELILMCRMLWRELGLVE
                     GRDLRLELNSLGQPDERRAHREALIAHFESHAAVLDEDAKRRLHSNPLRILDTKNPAM
                     QSIVESAPQLMSFLGEESLAHFNAVRCALDAAGLSYRINPRLVRGMDYYNLTVFEWVT
                     DRLGSQGTVCGGGRYDTLIEQLGGKPAPAVGWGLGIERLLDLLQQEGLVPPPPAPLAY
                     AVVPDAAALPRAVACVEALRAAGLSVVLHAAGKDG"
BASE COUNT         3134 a         5547 c         5748 g         3176 t
ORIGIN      
        1 tgccgatcct gcgcaatgcc gaccagatga gcttcgccga catcgagaag aagatttccg
       61 agtacggcaa gaaagccgct gaaggaaaac tggctctgga agagctgact ggtggcacgt
      121 tctcgatcag caatggaggg accttcggat ccatgctgtc gacaccgatc atcaacccgc
      181 cgcaatcggc catcctgggc gttcatgcga cgaaggaccg tgcggttgtc gagaacgggc
      241 agatcgtcgt gcgtccgatg aactacctcg cgatgtcgta cgaccaccgc atcatcgacg
      301 gccgtgaagc cgtcctgggg ctggtggcga tgaaggaagc gctggaagat ccggcacgtc
      361 tgctgttcga catctgattc gagatccgag gacctttcat gagcaagcaa ttcgacgtgg
      421 ttgtcattgg tgccggccct ggtggctatg tggcggccat ccgtgcagcg caactgggct
      481 tcagtgtcgc ctgcatcgac gagtggaaaa acgacaaggg cggtcccgcc ccgggtggaa
      541 cctgcacgaa tgtcggctgc attccgtcaa aggccctgct gcaatccagc gagtatttcg
      601 agcacgccga gcacagcttc gcagaccacg gcatcggcct gtcgaacctg agcatcgacg
      661 tgcgcaagat gctgggccgc aaggacgcgg tggtgaagca gaacaacgac ggcatcctct
      721 acctgttcaa gaagaacaag gtgacgttct tccatggccg cggtgctttc gtgaaggccg
      781 gtgacgccgg ttatgacatc aaggtcagcg gcgctgccga agaagtgatc acgggcaagc
      841 acatcgttgt cgcaactggc tcgaacgcgc gatcgctccc gggggccgcc ttcgacgagg
      901 acaagatcct ctccaacgac ggcgctttgc gcatcagcga ggtgccggag acactgggcg
      961 tcatcggctc gggcgtcatc gggctggaga tgggctctgt gtggcgtcgc ctcggtgccg
     1021 aggtgacggt gctcgaaggt cttcccacat tcctcggtgc gatcgatcag cagatcgcca
     1081 aggaggctca caaggccttc accaagcagg gcttgatgat cgagcttgga gtcaagatcg
     1141 acaaggtcac ttcaggcaag tcgagtgtca cgatcacgta taccaacgcc aagggagcag
     1201 cctgcaagct cgaggtcgac aaactgatcg tttcgatcgg ccgaggcccc aacactgttg
     1261 gcttgaatgc cgaagcagtg ggcctgaatc tcgatgagcg tggcgcgatc acggtcgacg
     1321 cagagtgccg gaccaacctc cccaatgtct gggcggtcgg tgacgtggtg cgcggaccga
     1381 tgctggcgca caaggcggag gaggagggcg tggccgtggc cgagcgtatc gctggacaac
     1441 atccgcacgt cgacttcaac accgtgcctt gggtgatcta cacatcgccg gagattgcgt
     1501 gggtcggcaa gaacgagcag cagctcaagg cggagggccg cgagttcaag gcgggtacct
     1561 tcccgttcct ggccaatggc cgcgcgcgtg ccatgggtga caccaccggc atggtcaaga
     1621 tgatcgccga cgcgaagacg gacgagatcc tcggcgtgca catcgtcggc ccgttcgcct
     1681 ccgaactgat cgccgaagcg gtggtggcga tggaattcaa ggcgagtgcc gaggacatcg
     1741 cgcgcatctg ccatgcacac ccttcgctga gcgaatcaac caaggaagcc gcgctggcgg
     1801 tagacaagcg aaccttgaac ttctgatcga atccgcagcc cgccccgggc tgcggtatcg
     1861 cccggggctg tctcgggttg tcgtgcgatc aaatctctcg gtgacccctc atgtccgtcc
     1921 ggcagctcta ccaacagacc ctcgccgacc gggggtattc agcagacccc gcccagctgg
     1981 cagcggtcgc atcgcttgag cgatgcgaga acgagtggat cgagtacaag tcccgtcggc
     2041 gcaacgcgct gaccaagttg ctgatccgtc ccgagatacc ccgcggcgtc tacatgtacg
     2101 gtggcgtggg tcgtggcaag agcttcctga tggactgctt cttcaactcc gtgccactcg
     2161 aacgcaaggc gcgcctgcac tttcatgaat tcatgcgcga ggtgcatcgc gaactggccg
     2221 atctgcacgg cacggtcaat ccgctcgagg tgctcggcca gcgcatgtcg cgtcggttcc
     2281 gcctgatctg tttcgacgag ttccacgtgg cggatatcac cgatgcgatg atcctgtacc
     2341 gactcctcga atcgttgttc aatcatcggg tgagcatcgt gacgacgtcc aacttccacc
     2401 cggacggcct ttatccgaat ggcctccatc gagaacgcat cctgccggcc attgctctgt
     2461 tgaaggatcg cctggagatc atcaatgtcg atgctggaac cgattaccgg cgcatgacgc
     2521 tcgaacaggt cgagatgtat cagacaccac tcggagaagc cgccgacgcc gcgatgacgg
     2581 ctgcgttcga gcgcctggcg gagacgaggg acgagaatcc gcggctgaac atcgagcatc
     2641 gggtcttgaa ggcgcgtcgc cgtgcagggg gtgtggtctg gttcgatttc caggaactgt
     2701 gcggcggtcc gcgttcgcag aacgactacc ttgaactggc gtcgcagttt cacaccgtcc
     2761 tggtgtcggg cgtggttcag atgtcgccgc gcctggcctc agaagctcga cgtttcacct
     2821 ggcttgtcga cgtcatgtac gaccgacgag tcaaactggt gctttcggcc gaagtgccgg
     2881 cagaagatct gtacacggaa ggccccctgg cgcacgagtt cccgcgcacc gtgtcgcgac
     2941 ttcatgaaat gagatccccc gaattcctgg ctttggcccg acgtgatgtg gagactgcgc
     3001 tcacatgaac cagaaaggtc gcccagtgtt tcgcggaatg ccttggctgt tgagcgcgct
     3061 gttggctttg agtcctctgg cctgggccgg cgattactcg agcgagaagg ctcgtatcaa
     3121 caaggccagg gacgaagccg atgccatcct ggccgccaag gaggcggagt gcaggcagcg
     3181 cttcgtcgtg aactcctgtc tgatcgaagc tcggcaacag catcgtgcag tggtgcagcc
     3241 cttgcgtgaa gaactgatcc tgctcgacga gaagcagcgc aagcagcgag cagcagatcg
     3301 cctcgatcgc atccgtgcga aatccgaccc cgcgtcgagc cggtccgatg ctgctgcttt
     3361 accggcggcc tccgcctcgg tgccgtcggc cgcggcgtcc cgttcttcgc cacgcccgct
     3421 gattcggccc cgactccggt cgtttggacc agcgagcgaa tctccgcttg ctgatatgcc
     3481 ggcggcattg ccggagcctt cgagcacacc agagagcgtc atggagatcc cggcggcaga
     3541 tcccgggcac gcgcgcaagc gcctcacacc gatgcccaat gcgaacgctc tcaaggactc
     3601 gacagatcga gcccgggaga tcgaggaacg ccgtgacaga gtgctcaagc ggaatgcaga
     3661 gaaggccgcc aggaagccac cggctgctcc gctccccgtt ccagccgctg cgtcgtccgt
     3721 ggctggctga ccgtttgcct cgaaggtcag gcggcgaggg cgtcgacgcg cacgagcgcg
     3781 agcgacaggt tgtcgccgcc gccacgcgcc cgttggcggg ccttgtcgat cagcatctcg
     3841 ctggcttcgc gtggcggtag cgcatcgacg atggcgccaa gttctcgcgg cgtgaagtag
     3901 tgccacagcc cgtcgctgca ggcgaggaca gtgtcaccgc gctccatgcg ctcgatgcgg
     3961 gtcaccgtca gtggaggatc ctgagtcgtt cccaggcaac cggtgagcag gttcgactga
     4021 ggatggctgt tcgcttcttc ttcggtcagc tgcccctcgt tgaccagtct ctgcacgaag
     4081 gagtggtcgc tggtgcggct gaccatctcg gcgccccgga agaagtagac ccgggaatcg
     4141 ccagcgtgca cgatgtggca ttcgcgtgtc gggctgatca ggaaggctgc caccgtactg
     4201 tgcggctctt cctcggatgt gatcgccgtc agcttgatca tcaggtgcgc ttcgagcaca
     4261 agctgcttca gagtttcggc cgggtcgtcc cggctgggcg cgtagcgctc gaaaagctgc
     4321 tgcgctgtca ggatgacctg atctgctgcc tttcgaccgc cgctcttgcc tcccatgccg
     4381 tcagccagca cgccgagcgc gcatcccggc acgcgtgcgt ggggaatgat ttcgacctga
     4441 tcctgctggt aggcgcggtc gccccggtgt attccggtgg ctgcggacag ccggtgcccc
     4501 tgcgcggttg aagtcatgca aaaactataa tcggttctcc tgttggaatc tcgtgaagag
     4561 ttcgcagtga tctcttgatg aacaccaacc tgcactcttt gccccgactc ctcatcgaac
     4621 tccgcatcga gcatgccgac ctcgatggac tgatcgatca ggccacgctc agcctgcccg
     4681 aggacgagtt gatgctgcgg cgactcaaga agcggcgcct ggcactgaag gatcagatct
     4741 cccgtctcga agccgaactc gatccgcccc agccggcatg acccgcagac cggtctcgga
     4801 gggcggtccg tgagcccgct gcgcgcggcg gtcatgcagg cgttctcggc cagcggggcc
     4861 ctgtcgcgtg ccgacccggc ctacagggag cgagaagtcc agttgcgcat ggccaccgcg
     4921 gtggccgacg ccatcgacac gcgcggcacc ctggtggccg aagccggaac aggtgtgggc
     4981 aagacgttcg cctatctggt gccgacgctc ctgtcagggg cacgtgcgct gatcagcact
     5041 gcaacgaaga gtcttcagga tcagctcttc ctgcgcgatc tgccgcggct tcgcgaatcg
     5101 ctcgcgctgc ccacgacgat ggcgctgctg aaaggccgag ccagctacct gtgcacgcat
     5161 cgcatggggc tggcccgcca ttcgaccgac ccctcggatc gctgggctgc acgcacgctg
     5221 gccaggatcg aagcctggtc gcagggcacg gcgaccggcg acctcgccga aatcgatggc
     5281 ctggacgagc gcagcagcgc gatcccgctg gttacctcga cgcgcgagaa ctgcctgggc
     5341 agcgagtgcc cggactatcg cagctgccac gtgatgaagg cccgccgaga tgccatggcc
     5401 gccgacatcg tcgtggtcaa ccatcacctg ttcttcgccg acatggctct gcgtgacagc
     5461 ggcgttgccg aactgctgcc cagtgtcgaa gtggccgtgt tcgacgaggc gcaccagttg
     5521 tcggaggcgg gcgttgcgtt tcttggcgtc accttgtcca gcggccaact gctcgatacc
     5581 gcgcgcgaca tgctcgccgt gggtctgcag caggcgcgtg gcctggcggc gtggcaggac
     5641 ctggctgctg cttgcgaccg gtctgcgcgg gagttgaggc tggcctgcgt tggcgtcacg
     5701 cgggccaccg aaggccgtgg gggccatgcg ccgcgtggtt cgatgaagct gcggtgggat
     5761 gatcgtgcgt ctcggccggc atttggcgtg gccctggacc aggcgggggc tgcctgcgcc
     5821 accgctcacg cggcgctcga cacggtgtcc gagctgtcgc cggacttcgc gaagctcgct
     5881 gatcggatgg cccggttgca cgaactcacc gagcgctttg ccgagtccgc tcagccgggt
     5941 caggttcgct ggattgaagt gagcgcgaat caatgccggc tcgtcgagtc tccgctcgac
     6001 atccgcgatg cgctgcgcga gcagatgatg tcggccccca aggcttggat attcacctcg
     6061 gccacgctgg gcgaggatga tcgactgtca tggtttaccg agccagccgg tctgacggac
     6121 gctgccacct tgagagtcgg cagcccgttc gattacgcgg agcatgcccg gctctatgtg
     6181 cccgggcagt tccccaagcc gaacgatccc gatcacccga cttgcgtgac tcgactggcc
     6241 gctcgttgcg cgcgtacgct gggcggacgt acctttgtcc tcacaaccac gttgcgggcg
     6301 ctgcaggcca tcggagacgg tctgacggct gatttcgaag ccgggggtga cgatatttcg
     6361 gtgctcgtcc aggggcagtt gccaaagcgg caattgatgc agatgttcct ggctaatccg
     6421 cgatcggtcc tgatcgggtc gcagactttc tgggaaggaa tcgacgtccc aggcgatgca
     6481 ttgcagtgcg tcctgatcga caagttgccg tttccgccgc cgagcgatcc gctggtcgag
     6541 gcacgcgtga aacgcctcga gaacgagggg cgcaatgcgt tcaccgacta tttcgttgcc
     6601 gaagcggcgg tgtccctgaa gcagggcgcc ggccgcttga tacgcagcga aaccgatcga
     6661 ggcttgctcg tggtgtgcga tccgcgcatg tcctcgatgg ggtatggaaa gcgcctgagg
     6721 ctggcgctgc ctgcgatgcc tcgcctcgaa tccgaggacg atgcaatggg ctggcttgcc
     6781 gggctggcaa gtgaacgatg aaatcgctgc ggggaaatgc gtcgggtcag ctgctcacca
     6841 caactgccac caggggcgag tcgatgactt gacaccttcc ccactgagaa atgcactgtt
     6901 cgggtagttc gtgcgcatca cgcgctcggc atcgtctcgc aactggtcga gaccgagccg
     6961 gtcatagctg cggatcagga tcgacagcgc ctcctccgtg gaaggcgagt attgaaactc
     7021 ctggatcgtc tgctgtgccc gattggcagc ggcgacgtag gcgcctctgc ggaaatagta
     7081 gcgagccacg tggacttcgt actcggccag cgcattcacg atgtaggcca tccgctgacg
     7141 cgcctccacg gaatacttcg actgcggata ctgatcaacc agctgcttga aagcctggaa
     7201 cgcatcgcga gacgcctgct ggtcgcgctc tgacaggttc tgtcgcgaca ggttgcccag
     7261 gaaacccagg ttgtcattga agttgatcaa gccttgcagg tacatcgcgt agtcgatcgc
     7321 ggggcttgtc ggatgtagct tgatgaagcg ctcgacggtg gccagcgcct gcgccttttc
     7381 gccggaacgg tacagcaagt aggagcgctc gagctgtgcc tgttgagcga ggatggtgcc
     7441 ggcgccgcgt ccttccagcg actcgtacat cttcgcggcc ttgtcgaagt ttcccgcgtc
     7501 ctgttcctcg cgggcctctg cgtacaattt ttccggagac cagtctgccg tgggatcctt
     7561 ggggccggag ctgcaacctg ccagtatcca gaccagagcg agccccaatc ccgtcagcgt
     7621 tgctggcctc atggcatggc gttcggtgca gcgcttgaaa ttcatgttcg actgtcccga
     7681 cggcccgccg gaatcccctt aaaaatgacc gccgagtata cggttcaccc catgcccgtc
     7741 ccgtctgccg cacccctcga agctggactg gcgatggatc cggacgacct cggcgagccc
     7801 gcaaacgacg agcctgtcga cgtcccggtg gagcggcgtc aggcaaaggt cacctccgaa
     7861 ctgcacggct cgagactcga caagaccttg gtctccatgg ccggggagtt ttcccgcaac
     7921 cacctacagt ccctgatcga agccggacat gtgtggatcg acgggcgccc gcaggcatcc
     7981 gcctcgcgca aggtgcttgc gggccaggtc gtgaccgtgg agctgacgcc gacccaggag
     8041 tgccaggcat tccggccaga ggcgatgtcg ctggacatcg tccacgagga cgaacacctg
     8101 atggttgtga acaagccggc cggtatggtc gttcacccgg ctgccggcaa ctggtcgggc
     8161 accttgctga acggcctgct tgcgcatcat gccggtgccg ccaccttgcc acgtgccggc
     8221 atcgtgcaca ggctggacaa ggatacgtcc ggactcatgg tggttggcaa gacgctttcg
     8281 gcggtcaccg cactcgtgcg acagatcgcc gcacgcgatg ttcgccggat ctacctggcg
     8341 atatgccacg gtcatttcga gcatcggcac ttgcgtatcg aagctccgat cggacgagat
     8401 ccacggtctc gggtgcgcat ggcggtggtg tcgtccggac gctcggcgcg taccgatgtg
     8461 gagcgcctcg ccgtgctgga cgacgaatcc gcacccgcgt tgagtgcact gcgctgcagc
     8521 ctgcacacgg gccgcacgca ccagattcgc gtgcatctgg cttcggtcgg gcacccgttg
     8581 cttgccgacg caacctacgg cggtcgggag ttgttcggca tgacgcgaca ggcgctgcat
     8641 gccacccgat tgggcttcgc tcatccggcc agcggcgagc cgctggtttt tgaggccccg
     8701 ccaccgcccg atctggccag cgtctggcat ctcctcacca gcgtaggttg agctgggttt
     8761 cctccggtac actcccctgg ctccgtcgtg aaatgaaatc cggcctgagg gtcaccgacg
     8821 agacagccgc cgctgcaccc atcgccgacg tgagagcctt tctcgacgat ccgcacggcc
     8881 gcttttgccg ccgcgacatc gcgcaaccct tctgaagggc ctgtgcttcg aggcgactga
     8941 acgagaacag atcctgaata tccaccggcc acggccggtc gaggacgacc acgagaccca
     9001 tgaataccca ggatgcaaag cgcgtccttc aaaccgcgct gatctgcgcc cgagagccgc
     9061 ttgcgatgcg cgagatgcgc gcgctgttcg ctgacgaact cggtacagac accctgcacg
     9121 agcttctcgg cgagctggtt cgtgaatggg agggcgaagg tgtcgagttg gtggcggtat
     9181 cgaccggctg gcgcttccag agtcgtccgg agatgcgtga gttcctggat cggctgcacc
     9241 cggaaaagcc gcccaagtat tcccgcgcgg tgatggagac cctggccatc attgcctaca
     9301 ggcaacctgt cacgcggggt gacattgaag acatccgcgg cgtcacagtg gccacccaga
     9361 tcgtcaagca gctcgaggac cgaggctggg ttgaggccat cggataccgc gaagcccccg
     9421 ggcgaccggc cttgctggcc accacccggc agttcctcga tgatctgggg ctggcaagcc
     9481 tcgagcaact tcccctgatc cagggggcag ctgcgttgcc tgaatccctg ggtgaactcc
     9541 tccctgatca gggtgtgctg cttgagcagc aggagtcgtt agatccggat cccgaaactg
     9601 caactccagc cgacgcaccc ttggtgaccc aggcacttga tctcttaccc gacccagcgg
     9661 cggacgccgc acccatggaa cgcccgaatg aatccaaatc ctgaccacac gcccgaaaac
     9721 gacagcgagg cgatacctgc cgacgttccg ggcgagggga ctgaagcgcc tgccaaaccg
     9781 cctcgtcgca aacgggctgc gaagacggaa gcgtccgtgt cctctggaga aagcatctcc
     9841 accgaggctg tcgatgcccc ggctgaagcc cccgaggcgt cggagcggcc ggctgtcgag
     9901 ggcgaagaag ctgtgcaggt cgcgggcgaa gcgcctaccg attccggcgc gcctgatccg
     9961 acccgcaggg cacgtggccc acgtcggcgt ggcccgcgtc cagacgacga aggtccgtcg
    10021 gtcacggctt ctgcgccaga tgatcaggcg gacgaaacgg attcgaccga gcctgtcttc
    10081 tccctggaac cggcagtccc tccgatgcag gtcggcgagg tgtttgccca ggtgttgtca
    10141 ggcgaattcg acatcgagcc ccctgcggaa gaagccgttg cgccaccgaa gcgcgttctg
    10201 cgtccggaac cggaagcgcc gaagctgcac aaggtgctgg cgcaatccgg aatcggctcg
    10261 cggcgtgaca tggagcaggc catcctggat ggccaggtcg aggtcaacgg gcagccggct
    10321 cacaccgggc agcgcatctc gttcggcgat caggtcaagg tcaatggcaa gccggtccgg
    10381 attcgcatca ttccgccgcc gccgcgcatc atcgcgtacc acaagcccac cggcgaagtg
    10441 gtgaccaacg acgacccaga acaccggccc acggtctttc gccgtctgcc tcggttgcct
    10501 cagggcaaat ggcagtcggt cggtcgcctc gacatgaata ccgaaggcct gctgctgttc
    10561 accaactcgg gagaactggc caaccggctc atgcatcccc gattcggcat cgaacgcgag
    10621 tacgccgtgc gggtgctggg gaccctcgat gcggagtcga aggcgatgtt gctggaggga
    10681 gtcaccatcg acggccagat ggccagtttc cgctccatcg agaacggtgg cggcgaagga
    10741 ctgaatcact ggtaccgggt ggtcatcacc gaaggacgaa accgcgaggt gcgcaagctg
    10801 ttcgaggccg tcgacctcac tgtgagcaga ttgattcgta tccgctacgg ttgcgtcgtg
    10861 ttgccgcggg gcctgcgtcg cggaaactgg gtcgatctgg aggacagcga tgtgcgggcg
    10921 atccgccgtc tggccggtgg ggaagccgaa ggaggtccgc gcgaagcacg acccaacccg
    10981 cgagacgggc agcaagcccg tccgcagcgc ggccgcgaca agcccccgca gggcgatcgc
    11041 cgcaacgaac ggccaccgcg tcaggggggc gatcgtccgg tcaacgaagg ccgaggcccc
    11101 cgccctcagc agtcggaatc gccgttgcgg gccgagcgtg tggtcgagga tcgtcctccg
    11161 cgggacgacg atctcgatga cgacttcgat ccgtcgcgaa tcccgaatcc tctggagcag
    11221 accttcgaca agcgctttgt ccagaacccg cgcacgccgg ccggaggacg caatttccgc
    11281 acgggcggtg gcggcttcgg tgcgggcggc agtgcgcccc ccgcgcagaa gaaaggcgac
    11341 ggacctcggc agcccgatcc gatgcagacg tctgtgggct acatcggtgg cgaggccttt
    11401 catcgcaagc atgcccgcaa cggcggtggt cagggcggtg gcggtggtgg cggccgcggc
    11461 ggaaaccgcg gaggcggcta cggcggtggt ggtggcggcg gcggcggaaa cagcggcggt
    11521 ggtggccgtc gcggtcgctg atttcgcggg tgcccacggt agaatccgag gctttgtcta
    11581 gctatctcca atcaggaaag aattccatgg ccatcgaacg cacgctctcc atcatcaagc
    11641 ccgacgctgt cgcgaaaaac gtcatcggcc agatctactc ccgcttcgaa ggcgctggtc
    11701 tcaaggtgat cgctgcgcgc atggcgcatc tgtcgcgtgg ggaggcggag gctttctacg
    11761 ccgtgcacaa ggcacgtccg ttcttcaagg atctggttga tttcatgatc tctggtccgg
    11821 tgatgatcca ggcactcgag ggagagaacg ccatcatgaa gaaccgcgat ttgatgggtg
    11881 caaccgatcc gaagaaggca gagaagggca cgatccgcgc cgacttcgcc gacagcatcg
    11941 acgccaacgc agtccacggc tcggatgcag ccgagacggc agccgtcgag atcgcgttct
    12001 tcttcccggg cgcgcaggtc tacagccgct gatccccggt cgctgattcc cgctgcatcg
    12061 gccatccggg ccccccatga gcgccgtcaa cctgctcgac ttcgatctgg ctggcctgac
    12121 ggcgtattgc gaacaactgg gtgaaaagcg cttccgcgca acgcagttgt tccgttgggt
    12181 gcatcagcgc gggatgtccg atttcgctcg gatgtcagac ctggcgaaat cgtttcggga
    12241 caagctcggc gcggttgccg cagttcgggt ccctgccgtg ctcagcgagc aggcttcgtc
    12301 ggatggcacg atcaagtggc tgttcgatgt cggccacggc gatgcagtcg aggcggtgtt
    12361 cattcccgag gacgaccgtg gcaccttgtg cgtgtcgtcg caggccgggt gtgccgtcgg
    12421 ctgccgtttc tgttcgacgg gccaccaggg cttcagccga aacctcagca cgtccgaaat
    12481 cgtcggccag ttgtggttcg ccgagcacca ccttcgccag cgtcttcatc tgcccgaggg
    12541 aacgagggcc atcaccaacg tcgtgatgat ggggatgggc gaaccgctgc agaactacgc
    12601 ggcgcttctg cccgccttgc gggtgatgct cgacgatcac ggctatggcc tgtcgcggcg
    12661 gcgtgtcact gtgtcgacgt ccggtgtcgt gccgatgatc gaccgtctgc gcgacgattg
    12721 ccccgtcgcg ctggctgtgt cgctgcacgc tccgaatgat gcgctgcgcg acgatctggt
    12781 gccgctgaac aagaagtatc cgctgtccga attgctggca gcctgcgagc gttacctcga
    12841 ggcagcgccg cgcgacttca tcactttcga atactgcatg ctcgatggtg tcaacgatcg
    12901 ccacgagcat gcccggcagc ttgtcgatct ggtgcagggc agggctcgtg atgcgcaggg
    12961 gcgtccgatc cacgcgaagc ccttgtcctg caagctgaat ctgattccct tcaatccatt
    13021 cccggcatcc gggctgatcc gcagcccccg ggcacaggtc cttgagtttg ctggcatcct
    13081 gcaggatgcc ggtatcgtga cgacgatacg caagacccgc ggtgacgaca tcgatgcggc
    13141 ctgcgggcaa cttgccggtg aagtgcagga tcgaacccgc atcgagcaac ggcgcgcggg
    13201 tcgcgcggcg attcccatcc tggctgtgaa cacgtcggca tcgcctgttg aagcgatccc
    13261 gatcaacgaa ggacacgcat gacgattccg gctgtcagca ccgatctgcg cggttggctc
    13321 tggaggtgtt ctgcaggcct ggtactgttg ggcgcgtggc ttttggcggg ctgcgccgca
    13381 ccgccgcctg ctgccgcggc cccgggttcc aaggagccgg tggtcaatgg cccggtgact
    13441 tccaccggca cgaaggacca catcacccaa tccgacgaga ccgatgcatc gcgtcgggct
    13501 caggtgcgac ttgaactggc aggtgcctat ttcggacggg gtcagatgac gactgcgctc
    13561 gacgaggtca agaaatcgat tgctgccgat ccgaacgtat cgggtgcctt caatctgcgc
    13621 gggctgatct acgccagtct gggcgatcac cgactggccg aggagagttt ccgccgcgcc
    13681 ttgcagatcg atccgcgcga cgcggattcg atgcagaact tcggctggta cctgtgccag
    13741 cagtcgcgct tcagcgaggc caacgtgctg ttccttcagg ctctggccgt tccgcaatac
    13801 caggacagtc cgcgcacgct gttgactcag ggcatctgcc aggcccgcag tggcaagaac
    13861 gacgaggcgg agcgcaccct cgtgcgcgcg tacgccctcg aaccgggcaa ccccgcaatc
    13921 gcagtgaacc tcagcgaagt gctgtaccgc aggggtgacc tggagcgcgc tcgtttctac
    13981 atccggcgtg tcaattcggt gcctaacctg gtcagtcctc agaccctgtg gcttgccgca
    14041 cgtatcgaga acaagtttgg caacccggcg ggtgtggctg atttcggtgg ccagctgcgc
    14101 aaccgattcc cggagtcgcc ggagtccacc gcgtacgtgg aagggcggtt caatgagtga
    14161 gcccaggacc acctcgggtg ccgctccggt tcctgcgcct gcctctgcga ggagtgccgg
    14221 cgacatgctg cgtgaagccc ggcaggccaa ggggctgcac atcgcagcgc tggctgcgtc
    14281 gatcaaggtg tcgcagcgca agctcgagtt gctggaggcc gacaggattt ccgagttgcc
    14341 cgatgccacc tttgcacgcg ccctcgctca gacggtgtgt cgcagtctga agatcgatcc
    14401 gactccggtt ctggctgcct tgccgcaggc gcccagctat cggctcgaag gggtggacga
    14461 aggcatcaat acgccattcc gtgaccgtcc agggcgacac gaacccaccg atttcgcggc
    14521 cgtcgcctcg ccagctgtct gggtgccggc attgatcgtc gtggcggcgc ttgtcgtcta
    14581 cctgttgccg tccgagtggg tggcgagtat gcagtcggcg gtttcgggtc agcctgctga
    14641 ccagccatcc gagcctgcgc cggcaacgcc cgccatcgcc gtggaacctg tgcagatcga
    14701 gcctgcgacc gctgcttcgt cgcctgcact cgtcgcttcc gatgctgcgg cgactaccac
    14761 ttccatcggc gagtcggcgt ctccgctgcc gggtgctgcc gtggtgtcgg aagcggctgc
    14821 ttcctcgccg gcagccgggc cgctgcaggt gaaggcggcg caaccgtcat gggtcgaagt
    14881 agtagatgcg cgttcgcagg tgctggtgtc tcgcatgctt cagccgggcg agacggtggt
    14941 gcttgatggg gccatgcctt tcaggctcaa ggtcggcaat gcagcgggca ccgaggtggc
    15001 ttttcgcggt caggcggtcg acctggcggc ggcgacgcgc gacaacgtgg cgcgcctcga
    15061 actgaaatag gtgatgagca tgagcgatct gcgtgacgtg agcgggtccg ccacggagag
    15121 cttcgcggcc ctggacgact tcatcgagcc ggcgcgacca gccccgaagc gcacgcgcca
    15181 ggcgcaggtg cggtggggct cgcgcgtcgt gacgatcggg ggggccgcgc acgtgcgcgt
    15241 ccagtcgatg accaacactg acaccgtcga cgtgatcggc acggcaatcc aggtcaagga
    15301 actggccgtt gcaggttcgg aactggtgcg gatcaccgtc aacactccgg aggcagctgc
    15361 ggctgttccg tatatccgcg aacagctcga ccgcatgggc atcgacgtgc cgctggtcgg
    15421 cgatttccac tacaacggcc accggctgct cagcgaacat ccggcctgtg ccgaggcact
    15481 gtcaaagtac cggatcaacc ccggcaacgt gggcaagggc gacaagcacg atcggcagtt
    15541 tgcgcagatg atcgagatcg cggccaagaa cgacaaggcc gtgcgcatcg gcgtcaactg
    15601 gggcagcctc gactccgaac tgctcacgca gatgatggat gacaacgcga agctcgccga
    15661 tccgcatccg ccgcagcaga tcatgtaccg cgcgttgatc acctcggcga tcacctcggc
    15721 caggcgggcc gaggcgctcg gcctgaatgg cgaccagatc atcctgtcgt gcaagatgtc
    15781 cggcgtgcag gatctggtca gcgtgtaccg cgcgctggcc aagcgctgcg attacgcgct
    15841 gcatctgggc ctcaccgaag ccggcatggg caccaagggc acggtggcct cagccaccgc
    15901 cctggcgctg ctgttgcagg atggcatagg cgacaccatc cgggtctcgc tcacgccgca
    15961 gcctggtgaa gcccgcgctc aggaggtggt ggtcgcgctc gagatattgc agtcgctcgg
    16021 gttgcggtca ttcaatccaa gcgtcacggc ctgcccaggc tgtggtcgca ccaccagcac
    16081 gacgtttcag gaactggcca agcagatcga tgaccacctg cgtgcccaga tgccggtttg
    16141 gcgggctcgc taccccggtg tcgaaaagct gaaggtcgct gtcatgggct gcatcgtcaa
    16201 cggtccgggc gaaagcaagc atgccgatat cggcatcagc ctgcccggga ccggcgaggc
    16261 tccggccgcg ccagtgttca tcgacggcga aaaggcgatg acgctgcgtg gcgacaacat
    16321 tgccggagag ttccacgcgc tcgtcgagag ctacatcgaa aagagatacg gcgcagcgac
    16381 cctgtctgac ggctgattgc cgcgcacaag acgttgttgc cgtgccgagc gcggcggccc
    16441 cattgccaca catcttctgt gcgcagagca cgcgtgactt gaaccattga gaacggaatg
    16501 agcgaaacca tccaggccgt caaaggcatg aacgacatcc tgccgcccga gtcggcaacc
    16561 tgggactggt tcgagggccg ggtgcgctcg ctgatggcgc ggcacggcta tgccggtgtg
    16621 cgcacgccga tcgtcgagcc gaccgcattg ttcgtgcggg ggcttggcga agtcaccgac
    16681 atcgtcgaga aagagatgta ctcgttcgtt gactcgatga atggagaccg cctgagcctg
    16741 cgacccgaga acacggccgg tgtcgtgcgt gccatcaccg agcactcgct tctgcgcgat
    16801 ggtggcaagc ggctctacta catcggtccg atgttcaggc acgagcgtcc gcagaagggg
    16861 cgctaccgcc agttccacca ggtcggagcg gaagccctcg gatacgccgg accggacgtc
    16921 gatgccgaac tgatcctgat gtgtcgcatg ctctggcgtg aactgggtct ggtggagggg
    16981 cgcgacttgc gactcgaact caacagcctc ggccagccag acgaacgtcg tgcccaccgc
    17041 gaggcattga tagcgcactt cgaatcccat gcagcggtgc tcgacgagga tgcgaagcga
    17101 cgtttgcaca gcaatccgct gcgtatcctc gacaccaaga accctgcgat gcagtccatc
    17161 gtcgaatctg caccgcaact gatgtcgttc cttggtgagg agtcactggc gcacttcaat
    17221 gcggtgcgct gtgcactcga tgcggccggg ctcagctacc gcatcaatcc gcggctggtg
    17281 cgcggcatgg actactacaa cctgaccgtg ttcgagtggg tcaccgaccg cctgggttcg
    17341 cagggcaccg tgtgcggagg cgggcgttac gacacgctca ttgaacagct cggcggcaag
    17401 cccgcgccgg cggtcggctg gggcctgggc atcgagcgtc tgctggacct gctgcagcag
    17461 gaaggactgg tgccgcctcc gccggccccg ttggcttatg cggtggtacc cgacgccgcg
    17521 gcgctgccgc gcgccgtggc ctgtgtcgaa gcgctgcgcg ccgcagggct cagcgtggtg
    17581 ttgcatgcgg ctggcaagga cggcc
//
LOCUS       DAOORV010000076        17492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3955_length_17492_cov_8.004359, whole genome shotgun sequence.
ACCESSION   DAOORV010000076 DAOORV010000000
VERSION     DAOORV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17492
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_17492_cov_8.004359"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKV56_11175"
     CDS             <1..987
                     /locus_tag="PKV56_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOX68420.1"
                     /translation="TAIDAALARSRTAYELNAAVGSDNASCFNLSATERGHGYNSFNQ
                     SGYRPSLQQTASGIYIQLFGGANINTPMVQSYMQWVKNRYRWQDLDNLGNFWSGNSMG
                     YYMWSSFKAMELLRQAGVPPSAGNIGPNEFGTLPAASAPACVVRQENKEPATFARPAV
                     FGAGAVGYYAGEPKGQYFDFAHQLISMQCANGSFTCTGFPGSWGDSAQASHNSYALLV
                     LQRSTGGGCVDTDGDGVCDDVDNCPAVANANQANTYGDARGDACEPRPLVSCDLDTDG
                     DVDSVDVKLITALRNKPASVNRKADIDGNGIININDARACVLKCTRLNCATQ"
     gene            1030..1653
                     /locus_tag="PKV56_11180"
     CDS             1030..1653
                     /locus_tag="PKV56_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX68421.1"
                     /translation="MFKLKQMARAAALVAGIGLGMAQSVMAAPISVSVVGAPLSVNSG
                     DFFSVDIVVSGITTEIVSAWDIDVAYDPSLLFADSFTTYLGPFGGFGFTDNDVDFTIP
                     GLIDAYLVSFLSDLDLTDLQCPGAVCSPSLTIASASFTALADGSPLVELVNWGDTNDV
                     KGANNEVIFGNAPEPVPEPASLALVGIAMVGMLAAPVLRRRKDAAKA"
     gene            complement(1813..2082)
                     /locus_tag="PKV56_11185"
     CDS             complement(1813..2082)
                     /locus_tag="PKV56_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68422.1"
                     /translation="MLFLLTNRTRPDLSSADYGELAALAKAFYASMPADVKIRGEWAA
                     VDRSVNYSLIEAPDIDTVHRIQAPFERFTDTVIVPVTTLTGWTAS"
     gene            complement(2087..2695)
                     /locus_tag="PKV56_11190"
     CDS             complement(2087..2695)
                     /locus_tag="PKV56_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68423.1"
                     /translation="MPRTPGRAALIIAAALCIPMVPFLVIGELPGERWLSASDAHAAR
                     FALVGGGLLAADVLLPIPSSIVGTMLGARLGWAAGWAATFAGLMVGQSAAYLVSRRLL
                     RQRRDALPEAPTLAALFLSRPVPVLAEAIVFSAGAARVSWPQFLVACGSGNLIYAGAL
                     ALNGAQLVPDAPLGIGLLLPMLLPAAGWLIWRKIRRTPPAER"
     gene            2770..4014
                     /locus_tag="PKV56_11195"
     CDS             2770..4014
                     /locus_tag="PKV56_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68424.1"
                     /translation="MAELITLTGSPSLKVQVMCEGIRYTAALGQATAHSMPNYYPYRF
                     KEGEHDPTGKGIVTIPYLINTPDGTEIRILGNGDSPWHVEGNLEQGYLLIDDRSGCTV
                     PIEFEPLRPWLTQKTTDGLPFAQAGVTTHGDMLVINVAPGCEYFLHKLDGVSMRCTFC
                     AYGAPDERTLHLGQKTGQLEIPPPTLDRMREALNAVIDQAEIRHIYLVGGSLTDARRE
                     GERFLQLARYVKQANTRGIPVTLGSGSIPDDLIAQFHAEDLVQHVCFNLEMWSEPLFA
                     KVCPGKNRYVGYQRWIESLETAVKYWGRGNVYSAMVAGIELEPEHGLEWEEAARIALQ
                     GAEDLCSRGIIPIYSLVWPVGGRQRSDFHARIRSYFETLSSGYHAIRQRHGLTVSEGF
                     MCHRCAYMQLECDLDRTTMEVA"
     gene            4011..5033
                     /locus_tag="PKV56_11200"
     CDS             4011..5033
                     /locus_tag="PKV56_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOX68425.1"
                     /translation="MSAPDRSTARGPDPSSIIRLSTSYWESQTLLTANRLRVFDVMAD
                     GARTADEVATELHLDPRGTALFLRACVGLGFLEEAAGRFQNTPVAATFLVSRSPAFMG
                     NVIRYSDQLYGAWGQLEGSLRAGKPALPAETYLGDDPARTRGFVMAMHERALGIARVL
                     VNVLDLQGRRSMLDVGGGPGTYSVLLTERFPGLRSEVLELPGVAAVARELVASAGASD
                     RVVLRDGDYHSSDFGSGKDVVLMSGMFHRESEATCRGLIERASACLEPGGLLVVSDVF
                     TDEGGALPTFAAMFGLNMMLTAPDGGVHADADVMRWMGDCGFRDMRMVPLPPPMPHRV
                     VMGLKP"
     gene            5030..5731
                     /locus_tag="PKV56_11205"
     CDS             5030..5731
                     /locus_tag="PKV56_11205"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOX68426.1"
                     /translation="MNRRDGLMAALVWLAATGVTVVQAQAPAPPVGELKPLGQDRYQI
                     GRIVIDKRAGVFTVPGRVHVVGKPLEYLATSPAGMKAYESLLELDTTGSEFNLACILI
                     GLERDPNQVPWQQMRQVQQLAGQKINLSIAWSEGGKRRQVSAAEALLNADSGVKPESV
                     EWVYTGSPTSEAYGRFMADDTGTLIGFVHDSNSIIESRAGIGIGAYGSVRGHPMLPPI
                     GSAIELIVESTAPTK"
     gene            5728..6468
                     /locus_tag="PKV56_11210"
     CDS             5728..6468
                     /locus_tag="PKV56_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68427.1"
                     /translation="MIAVRWAMLAVLTLLMSAAFGATAADLQPSAQRVAVTAIVALLA
                     LLFWPGSAATRRQTVLRIAGWSLAAAGTAAVVLRTFGAAGQPLAATLGSCAMLLALLL
                     MMQALAAMLETYLRGPSRPSDEAREAAGFVVTILLALLGSLPLWFGPASELLSVRLDW
                     VVDAALAVSPLTHLAVASGNDLLHNEWLYQHSNLAALPVSYPDLTPLVWSYATACSLL
                     ALGALAAGRRRRAVNDPAPTDLTQEKPR"
     gene            6465..7553
                     /locus_tag="PKV56_11215"
     CDS             6465..7553
                     /locus_tag="PKV56_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOX68428.1"
                     /translation="MMKRRHLLVLPACAVLAAPSVHAADAMDPELRRKAKRAVAGGLH
                     YLRGQQAPDGSVLKSVGITALALRAFLESPEKYNEADGAFITRQVDYLLANAKSDGSI
                     SASLQSTAYNTAVAMSALAATKNPQHDAVIAAARKFLIGHQIDEEEGYKTDHVYYGGI
                     GYGGGERPDLSNLYMSLEGLKSTSSDPKDPVWQKALTFVNRSQNRSESNDQKWAANDG
                     GFTYRPGTNPPEYDNGTSSYGGMTAAGLLSLLFAGVDKTDPRVQAAYKWMTANYTLEF
                     NPGTNKKHGLFYFYNAFSKVMAAYGEDTFTDGKGQTHNWRNEIAQKLMSLQSADGSWA
                     NADSKAWWEDRPQLVTAWSVIALEQALK"
     gene            7558..9018
                     /locus_tag="PKV56_11220"
     CDS             7558..9018
                     /locus_tag="PKV56_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68429.1"
                     /translation="MLRCLLLIGLALCGLAHAIEPPRIDLTAAPAWKGWSRPGRATEI
                     DIRVTADTPTKTTLEVAAGRQVVRAELDLQPGRTVRLQIPVASTERIALTVGAPAASV
                     VRRDVGIAQSESPVLGVGLVTDEAVALEGFHTVLLGADDLPRNARAYSSVDALILDAQ
                     TLGALDQRQLGALFEHAAACGRIVVLNANERVLRLLEGAGGCGGSTLMSAATLADATD
                     RLKASLATSMPPAVSSAGLGELTRAAPSSWHRVVLGLAVYFAAAALVFLFFTSLPMLL
                     LMPVLAAAAALALPHAMQPSSQLVVWSEGESGARFAEYHASQWFTGLVRDRRRVPIPP
                     QLAPAAQPCAPNRAMRFEFDAGRGRPTFAEFETRLFDQTALCYSGSFPMTRAIEMHER
                     PDGSRDIRNVGAIAWPSGWLLGGGLASDLPALGPGASSQISPQAGRPPREAAMRMALR
                     RTPSDASAALWELELGGVVDAPIESKGWLHVSVPRR"
     gene            9015..9500
                     /locus_tag="PKV56_11225"
     CDS             9015..9500
                     /locus_tag="PKV56_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68430.1"
                     /translation="MKSVPDPRLSALVGLALAATVALWWLGSTRIALDQAGDASRAAA
                     AALLALWVVRGMVLAPLGLRAGALSGWRAGAAAAALLLAPAWPLLILVWSASTVPLLP
                     AALVELSLLSAGVVLPLLGQGLRRALGRPELAEVVATALGLALASTAWVLRDLWVWAP
                     P"
     gene            9508..10647
                     /locus_tag="PKV56_11230"
     CDS             9508..10647
                     /locus_tag="PKV56_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68431.1"
                     /translation="MAALPQTLIEAVRRRLWLGQFLAAARLALWGTAGLMLLTAAVHL
                     AGWPLSPDLMFLALAFVWSVMAARAAWQRPVDAACALWADRHLGGASAYTTLLELDSP
                     GPGTPPSHAVRHLEAFAAAQVPHSLRLLAQRRDALRLSRPLLLALVCAALASLVMMLP
                     GPDPKPQPPANLAATSGLTDRAAPDTDAPVSAELVSEISSALRATAADAAEAPERRGA
                     GDPATSGSDRAGDDGGSAAAAPDALAQRPAPADSGSPAVNPGQAAAAPGASRLAGSGS
                     GQDAGDSRGEPGGVGVARTPRSTIPTQAVELAARRPSGERRADPDQAATYDEELSMRT
                     AASAARRADAAPAAASPPLAAPEMWLSPAETNYVQAWLKASARHR"
     gene            10644..11645
                     /locus_tag="PKV56_11235"
     CDS             10644..11645
                     /locus_tag="PKV56_11235"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68432.1"
                     /translation="MTANPFSDSQYADAHRQLQDLRTLLDQAIVGQSELVTHLVTAVF
                     AGGHVLIEGLPGLGKTHLAKAIAAALGLRWARVQCTPDLMPADVTGAEIYVTTAGGAS
                     SFEFRPGPVFAQLVLVDEINRATPRTQAALLEAMQERQVTNAGVSHRLPVPFCVLATQ
                     NPIELEGTYPLPEAQLDRFMFKLTVPFPTRESLRGMLDVSLDAEPSDTMQPIAGPDDV
                     LRISELCRTLLLGERCKEAAVDLIVSTQPAVGGDPADARRHIRYGASPRALQSLVRAA
                     RVRALMAGRTHVDIDDLAAIALPVLRHRVLLRLESELDGLEVDSTLASIIDGWRLRL"
     gene            11627..12526
                     /locus_tag="PKV56_11240"
     CDS             11627..12526
                     /locus_tag="PKV56_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX68433.1"
                     /translation="MATSALIPAALPSEPELRSFASAASQLLVERQPRSSGSRAVSRR
                     AGVGLQHFDHRDYAPGDEVRHIDWRQSARRRQPIVRRFESESVADWTILLDASSSMAV
                     QGAAKWRSAVHVAAAMSYALLQMGHRVGLLAFGARVLSECPRGRGQHHYAAIARLLLS
                     LQPAPAGERSELGVCTGHLRGASTAFALSDFLSSDEMRRDLSAIGQRCTALHAMQLSD
                     SAETRLRPLGDVDLVDVETGASMPAHASEHANALAADERAARTARLRGFCARSGVAFS
                     DWDIANPWQHALLRHLVRARSNC"
     gene            12541..14136
                     /locus_tag="PKV56_11245"
     CDS             12541..14136
                     /locus_tag="PKV56_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68434.1"
                     /translation="MWLLGLLLLPVIRWLHRGGRHRRAMPVSSLSLWAGAAASAPAAG
                     ERTPPDPAWRHRALLAALIFLALAEPQLPVQRTRVTLWVDDSLSMQTREARGTRISEG
                     LAQARRLLSEGGYTEVDVRALGDPWRSLGPPGDRTGANLSAKAGLKEPTPPPAALLRG
                     DRLHWLITDGTDAALSGWPDGRRPDRIIEVAGTSRNAGLERVSARRSPNDPDRIDLLL
                     KVTNGGNEAETREVVFITDAGESARSRQRIEPQTSTFVTASIAESSRVRVRLEPGDAL
                     VEDDTIDLDLAPLRKRRVATDSTCPGTVVAAVASHPGLAVAAADATDVQAALDCGSRA
                     SARDLATVRVVADIVPTSLQGPLQWSASLPESSRISLSIGSLQTAARLRVRPGDAVLL
                     AAADEPVIVMGRSGPAKLIETSLDLGSEAASRGPTVPLLVNLMFETVIGGPLLDRIAI
                     LDRGPASVLVVPSAPASAAPQAPPVLYLAASGGKPASKAAAVHRDGAWPLLAAAVLVL
                     LWEIVSLCRQWIRLNGSTGVAFE"
     gene            14133..16586
                     /locus_tag="PKV56_11250"
     CDS             14133..16586
                     /locus_tag="PKV56_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX68435.1"
                     /translation="MTGAGSRTSIALALQLAALVALSLSLLGVAWLDPRSKPRALLLV
                     DRSQSVPRAAADKAIAEVMQAARAGRGREVQLLEFAGRLAVPVAQANDAVTDLEPSVT
                     NIEVALDAALAAHAQEAIASVVVISDGLENVGDAARALRAVREARLPLQWIPVGRDPP
                     QTRIAEVLAPARALLGQRIPITVQLAGQLDPPVRVKATLRTAGGDTQVASSLSGKAGL
                     ATIEFDPRSSGAVLVDVAVEDAESGRTFHATPDAAVIDVAPRASILYVQGSSGALAHS
                     LRQGGWSLDLVPAARLDARTDGLAGYQAVVLEDVAVSDAGPRFWSALVDAVKSRGLGL
                     LVLGGERSFARGGYRGSVLESVLPVLSEPAALDEPAAILFAVDKSGSMGQGSGGVDRL
                     ALAQRAVIETARGLTARDSLGLLVFDVEPRLLIPLGPSQAGIRALERDWQSSPNGGTK
                     LAPALEAAIAELERSQAARRMLVVVTDGFVDGAPVDQLRARLERSRIELIALAVGPDA
                     DISALERVAGAEAGLVLRVNEAAELPVVMRAGLERRRARIERGTIAAEQHQAFPFPPG
                     TWKDWPAVAAHAVTRPQPDAVVAVRSHRGEPLIAFHESGPGRVAAVTSGLGPWTPRWL
                     SWREWPQLAGGLATWVSGAPQGAQIGLSVSDLPQDLQIDADLPGHAGRAESDDFSVVV
                     TTPASPSRSIAMEHVGPGRMRALLPEAGPGLYTFVLSTPLGTQRQLHLRHHRAENETW
                     GINPRLAAWTNDGLVGAWTPGELAAPPPSDGGEDRLPVDRTLLALALALFLSGVLVDR
                     TRLPRAFVSDRLRRLRTRT"
     gene            complement(16598..17338)
                     /locus_tag="PKV56_11255"
     CDS             complement(16598..17338)
                     /locus_tag="PKV56_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX68436.1"
                     /translation="MLSVRSLSRLIGQRAVVSEVSFDMAPGEIVAVLGPSGSGKTSLL
                     RMIAGFDAPTSGSVHLHGREASAAGRVLVAPEHRVLALAFQDATLFSHLDAVGNAAFA
                     IRATDKTSRLEKARAALRDMGLAGMDGRDVATLSGGEAQRVSLARALAADAKLLLLDE
                     PFGNVDRLTRIDLVSRLKARLANGLGAMIITHDPADAIELGARVLLMRDGRVMADGSH
                     ETITAGQRGDWARSFLLAGHIAGTAQHS"
BASE COUNT         2635 a         5873 c         6085 g         2899 t
ORIGIN      
        1 accgccatcg acgccgcgct ggctcgtagc cgcacggcct atgaactgaa cgcggcggtc
       61 ggttccgaca atgccagctg cttcaactta agcgccaccg agcgaggcca cggctacaac
      121 tcgtttaacc agtcgggcta ccgtccgtcg ctgcagcaaa cggcctccgg catctacatc
      181 cagttgttcg gcggcgccaa catcaacacg ccgatggttc agagctacat gcagtgggtg
      241 aagaaccgct accgctggca ggacctggac aacctgggga atttctggtc cggcaactcc
      301 atgggctact acatgtggag ttcgttcaag gccatggaac tgttgcgtca ggcgggtgtg
      361 ccgccgtccg ccggcaacat cggcccgaac gagttcggca ccctgccggc tgccagcgct
      421 ccggcgtgcg tggtgcgcca ggagaacaag gaaccggcca ccttcgcgcg gccggccgtg
      481 ttcggcgcgg gcgcggtcgg ctactacgcc ggcgaaccca agggccagta cttcgacttt
      541 gcgcaccagc tgatcagcat gcagtgtgcg aacggctcgt tcacctgcac cggcttcccg
      601 ggttcgtggg gcgactccgc gcaggcttcg cacaactcgt acgctctgct cgtgcttcag
      661 cgctcgacgg gtggcggctg cgtcgacacc gatggtgacg gcgtttgcga cgacgttgac
      721 aactgcccgg ccgttgccaa cgcgaatcag gcgaacacct acggcgatgc gcgcggcgat
      781 gcctgcgagc cgcgtcctct tgtgagttgc gatctggata ccgacggtga tgtcgacagc
      841 gtcgatgtca agctgatcac cgcgctgcgc aacaagccag cgtcggtcaa tcggaaggcg
      901 gatatcgatg gcaacgggat catcaacatc aacgatgccc gggcttgcgt gctcaagtgc
      961 actcgtttga actgtgcaac gcaataaacc gcatctgacg attcactgca attactgaaa
     1021 ggaaagatca tgttcaaact caagcaaatg gcccgcgctg cggccttggt ggccgggatc
     1081 ggcctcggca tggcccagtc ggtgatggcc gcacccatca gcgtcagtgt tgtcggggcg
     1141 ccattgagtg ttaactcagg cgatttcttc agcgtggata tcgtcgtgtc aggcatcacg
     1201 accgaaatcg tcagcgcgtg ggatatcgac gtggcctacg atccgtcgct gctgttcgca
     1261 gattcgttta ctacgtatct cggccccttc ggcggtttcg gttttacaga caatgacgtg
     1321 gatttcacca tccccggtct gatcgacgcc tatctggtgt catttttgag tgacttggat
     1381 ctgactgatc tgcaatgccc tggcgctgtt tgcagtccga gcctcacgat tgcgtctgcc
     1441 agtttcacgg ctcttgccga cggctcgccg ctggttgaac tcgtcaattg gggtgacacc
     1501 aacgatgtga agggcgccaa caacgaagtc atctttggca acgctcctga gccagttccc
     1561 gagcccgcgt cgctcgcgtt ggtcggcatc gcgatggttg gcatgctggc ggctccggtg
     1621 ctgcgccgtc gcaaggatgc agccaaggct taactgctga tttgcaggca agcgacggca
     1681 aaggccgcca cgaaagtggc ggcctttttt gttcagcttg ctccgtcttg cccaggccag
     1741 atgtggccgc gttgtgccaa cagtgtgggg tcgggcgtta gcaacagtgg cggctatgcc
     1801 gggccagccc ggtcagctgg ccgtccagcc cgtgagggtg gtcaccggca cgatgacggt
     1861 gtcggtgaat cgctcgaacg gcgcctggat gcggtgcacg gtgtcgatgt cgggcgcctc
     1921 gatcagcgag tagttgaccg agcggtcgac ggcggcccac tcgccgcgaa tcttcacgtc
     1981 ggccggcatc gatgcataga aggccttggc cagcgcggcg agttcgccgt agtcggccga
     2041 gctcaggtcg ggtcgggtgc gattggtcag caggaacagc atggcgtcat ctttctgcag
     2101 gaggggtgcg gcgtatcttg cgccagatca gccagccggc ggcgggcagc agcatgggca
     2161 gcagcagccc gatgcccagc ggggcgtcgg gcacgagttg cgccccgttg agcgccagcg
     2221 cgccggcgta gatcaggttg cccgatccgc aggccacgag gaactgcggc cagctcacgc
     2281 gcgccgcccc cgcgctgaag acgatcgcct cggcgagcac cggaacgggc cggctgagga
     2341 acagcgcggc cagcgtcggc gcttcgggca gcgcgtcgcg gcgctgtcgc agcaggcgcc
     2401 ggctgacgag gtaggccgcc gactgcccga ccatcaggcc cgcgaacgtg gcggcccagc
     2461 cggcggccca gcccaggcgc gccccgagca tcgtgccgac gatgctcgac gggatcggca
     2521 gcagcacatc ggccgccagc agcccgccgc ccaccagcgc gaagcgcgcg gcatgggcgt
     2581 cgctggcgga cagccagcgc tcgccgggaa gctcgccgat cacgaggaac ggcaccatcg
     2641 gaatacacag cgcggcggca ataatcaacg cggctctgcc gggggtgcgt ggcatcatcg
     2701 aatgacgtta tcagaagagc acgcgggcct gcgcgcccgc ttccacgctc gattcacgca
     2761 gggaaactca tggccgaatt gatcacgctc accggctcgc cgtcgctgaa ggttcaggtg
     2821 atgtgcgagg gcatccgcta cacggccgct ctcggccagg ccaccgccca ttcgatgccc
     2881 aactactacc cctaccggtt caaggaaggg gagcacgatc cgacgggcaa gggcatcgtg
     2941 accatcccgt acctgatcaa cacgccggac ggcaccgaga tccgcatcct cggcaatggc
     3001 gattcgccgt ggcatgtcga aggcaacctg gagcagggct acctgctgat cgacgaccgc
     3061 agcggctgca cggtgccgat cgagttcgag ccgttgcggc catggctcac gcagaagacg
     3121 accgacggcc tgccgttcgc gcaggccggc gtgaccacgc acggcgacat gctggtgatc
     3181 aacgtggcgc cgggctgcga gtacttcctc cacaagctgg acggcgtctc gatgcgctgc
     3241 accttctgcg cctacggggc gcccgacgag cgcacgctgc acctcgggca gaagacggga
     3301 cagctcgaga tcccgccgcc gacgctcgat cgcatgcgcg aggcgttgaa cgccgtgatc
     3361 gaccaggccg agatccggca catctacctg gtgggtggct cgctgaccga cgcgcgcagg
     3421 gaaggcgagc gcttcctgca actcgcgcgg tacgtcaagc aggcgaacac gcgaggcatt
     3481 ccggtgacgc tgggctcggg gtcgatcccc gatgacctga tcgcgcagtt ccacgccgag
     3541 gatctggtgc agcacgtctg cttcaacctc gagatgtggt cggagccgct gttcgcgaag
     3601 gtgtgccccg gcaagaaccg ttacgtcggc taccagcgct ggatcgagtc gctcgagacc
     3661 gcggtcaaat actgggggcg cggcaacgtc tactcggcga tggtggcggg catcgagctc
     3721 gaacccgagc acggcctgga atgggaagaa gccgcgcgca ttgccctgca gggcgcggaa
     3781 gacctgtgca gccgcgggat cattccgatc tactcgctgg tgtggccggt gggtggccgc
     3841 cagcgctccg acttccatgc gcgcatccgc agctacttcg agacgctgag ctcgggctac
     3901 cacgcgatac gccagcgcca cgggctgacg gtctcggagg ggttcatgtg ccatcgctgc
     3961 gcgtacatgc aactcgagtg cgacctcgac cgcaccacga tggaggtcgc atgagcgcgc
     4021 cagatcgttc cactgcccgg ggccccgatc cctcctcgat cattcgcctg agcacttcgt
     4081 actgggagag ccagacgctg ctgacggcaa accggctgcg ggtcttcgat gtcatggccg
     4141 acggtgcccg cacggccgac gaggtggcga ccgaactgca cctcgatccg cgcggcacgg
     4201 cgctgttcct gcgcgcctgc gtcgggctgg gctttctcga agaggcggcc ggccgcttcc
     4261 agaacacgcc ggtcgcggcc acgttcctgg tctcgcgcag cccggcgttc atgggcaacg
     4321 tcatcaggta cagcgaccag ctctacggtg cctggggcca gctcgaaggc tcgctgcgtg
     4381 ccggcaagcc ggcgctgccc gccgagacct acctgggcga cgacccggcg cgcacgcgcg
     4441 gcttcgtgat ggcgatgcac gagcgcgcgc tcggcatcgc gcgcgtgctg gtcaacgtgc
     4501 tggacctgca gggccgccgc agcatgctcg acgtcggcgg tgggccgggc acctactcgg
     4561 tgctgctcac cgagcgattt ccggggctgc gctccgaggt gctcgaactg cccggcgtgg
     4621 ccgcggtggc gcgcgagctg gtcgcgtcgg cgggcgccag cgaccgcgtg gtgctgcgcg
     4681 acggcgatta ccacagcagc gatttcggct cgggcaagga cgtggtgctg atgtccggca
     4741 tgttccaccg ggagagcgag gccacctgcc gcgggctgat cgagcgcgcc tcggcctgtc
     4801 tcgagcccgg cggcctgctg gtggtcagcg atgtgttcac cgacgagggc ggggccctgc
     4861 ccaccttcgc cgcgatgttc ggcctgaaca tgatgctgac ggctcccgat ggcggcgtgc
     4921 atgccgacgc cgacgtgatg cgctggatgg gtgactgcgg cttccgcgac atgcgcatgg
     4981 tgccgctgcc gccgcccatg ccgcaccggg tcgtgatggg actgaagccg tgaatcgacg
     5041 cgacggactg atggccgcgc tggtttggct ggctgctacc ggcgtgaccg tggtgcaggc
     5101 gcaggccccg gctccgcccg tgggcgagct gaagccgctg ggccaggacc gctaccagat
     5161 cggacgcatc gtcatcgaca agcgtgcggg tgtcttcacc gtgccgggcc gcgtgcacgt
     5221 ggtcggcaag ccgctcgagt acctggccac cagcccggcg ggcatgaagg cctacgaatc
     5281 gctgctggag ctcgacacca ccgggagcga gttcaacctc gcgtgcatcc tgatcggcct
     5341 ggaacgcgat ccgaaccagg tgccctggca gcagatgagg caggtgcagc aactcgccgg
     5401 ccagaagatc aacctgtcga tcgcctggtc ggagggcggc aagcgccgcc aggtgtcggc
     5461 ggcggaggcg ttgctcaatg cggattccgg cgtgaagccc gaatcggtcg aatgggtcta
     5521 cacgggatcc cccacgtccg aagcctatgg ccgtttcatg gccgatgaca ccggaacgct
     5581 gatcggcttc gtgcacgact ccaacagcat catcgaatcc agggctggca tcggcatcgg
     5641 cgcctacgga tccgtgcgcg gccacccgat gctgccgccg atcggctcgg ccatcgagct
     5701 gatcgtcgaa tcgacggccc cgaccaagtg atcgcagtgc gctgggcgat gctggctgtg
     5761 ctgaccttgc tgatgtccgc tgccttcggc gccacggcgg ccgatctgca accgtctgcc
     5821 caacgggtgg ccgtgacggc catcgtcgcc ttgctggccc tgcttttctg gccgggaagc
     5881 gccgccacgc gcaggcagac ggttctgcgc atcgccggct ggtcgctcgc ggcggcgggc
     5941 acggcggctg tcgtgcttcg caccttcggt gccgcggggc agcctctggc cgccaccctc
     6001 ggaagttgcg cgatgctgct ggcgctgctg ctgatgatgc aggcgctggc cgcgatgctc
     6061 gagacgtact tgcggggtcc atcacggccc tcggacgagg cgcgcgaggc ggccggcttc
     6121 gtcgtgacga tcctgctggc cttgctgggt tcgctgccgc tgtggttcgg cccggcgagc
     6181 gaactgcttt cggttcggct tgactgggtc gtcgacgcag ccctcgcggt cagcccgctc
     6241 acccatctgg cagtggcaag cggcaacgac ctgctgcaca acgagtggct ctaccagcac
     6301 tccaatcttg cggccttgcc cgtgtcctat cccgacctga cccccctggt ctggtcctat
     6361 gcgacggcgt gctcgctgct ggcgctcggt gcgctcgctg cagggcgcag gcgtcgtgcc
     6421 gtcaacgatc cggcccccac cgatctcacc caggagaaac ctcgatgatg aaaagaagac
     6481 acctgctcgt cctgcctgcc tgcgctgtgc tcgcggcgcc ttccgtgcat gccgcagatg
     6541 ccatggaccc cgagttgcgc cgcaaggcca agcgcgcggt ggccggtggc ctgcactacc
     6601 tgcggggcca gcaggccccg gacggttcgg tgctcaagtc cgtcggcatc acggcactgg
     6661 cattgcgtgc cttcctcgag agcccggaga agtacaacga agccgacggt gcgttcatca
     6721 cgcgccaggt cgattacctg ctcgcgaatg ccaagagcga tggctcgatc agcgcgtcgc
     6781 tgcagagcac cgcctacaac acggccgtcg cgatgagcgc gctggccgcg acgaagaacc
     6841 cgcagcacga cgctgtcatc gccgccgcac gcaagttcct gatcggtcac cagatcgacg
     6901 aggaagaagg ctacaagacc gaccacgtct actacggcgg catcggctac ggtggtggcg
     6961 agcgccccga cctgtcgaac ctgtacatgt cgctggaggg gctgaagtcc acatcctccg
     7021 atcccaagga tccggtctgg cagaaggcgc tgaccttcgt caaccgctcc cagaatcgca
     7081 gcgagagcaa cgaccagaag tgggcggcca acgatggcgg cttcacctac cggccgggca
     7141 ccaacccgcc cgagtacgac aacggcacgt cctcgtacgg gggcatgacg gctgccggcc
     7201 tgctcagcct gctctttgcc ggcgtggaca agaccgatcc gcgcgtgcag gcggcctaca
     7261 agtggatgac cgcgaactac acgctcgaat tcaatccggg gacgaacaag aagcacggcc
     7321 tcttctattt ctacaacgcc ttctcgaaag tgatggcggc ctacggagag gacacgttca
     7381 ccgatggcaa ggggcagacg cacaactggc gcaacgagat tgcgcagaag ctgatgagtc
     7441 tgcagtcggc cgatggcagc tgggccaatg ccgattcgaa agcctggtgg gaggaccgcc
     7501 cgcaactcgt caccgcgtgg tccgtgatcg ccctcgagca agcgctgaag tgacacggtg
     7561 ttgcgctgcc tgctgctcat cgggctggcg ctctgcggtc tggcgcatgc gatcgagccg
     7621 ccgcgcatcg acctgaccgc ggcaccagcc tggaagggct ggtcgcgccc cggacgcgcc
     7681 acggagatcg acatccgtgt gaccgccgac accccgacga agaccacgct cgaggtggct
     7741 gccggccgcc aggtcgtgcg agccgaactc gatctgcagc cgggccgcac ggtgcggctc
     7801 cagatcccgg tggcttctac cgagcggatc gcgttgaccg tgggggcgcc ggccgcatcc
     7861 gtcgtgcgcc gtgacgtggg catcgcgcag tccgagtccc cggtgctcgg tgtcggcctc
     7921 gtgacggacg aggcggtcgc gcttgaaggg ttccacacgg tcctgctcgg cgcggatgac
     7981 ctgccacgca acgcacgggc ctattcgagt gtcgatgcac tcatcctgga cgcgcagacg
     8041 ctgggtgcac tcgatcaacg acaactcggc gcgctgttcg agcacgcggc cgcgtgtggc
     8101 cgcatcgtgg tgctgaacgc gaacgagcgc gtgcttcgat tgctggaggg ggcgggtggg
     8161 tgcgggggca gcacgctgat gagcgccgcg acgctggcgg atgccacgga caggctcaag
     8221 gcgtcgctgg ccacgagcat gccaccggcg gtgtcatccg ccggactggg tgaattgaca
     8281 cgggctgcgc cctcgagctg gcatcgcgtg gtcctggggc tggcggtcta cttcgcggcg
     8341 gcggcgctgg tcttcctgtt cttcacctcg ctgccgatgc tgttgctgat gcctgtgctt
     8401 gcggccgcag cggcgctggc tctgccgcat gcgatgcagc cttcgtcgca actggtcgtc
     8461 tggagcgagg gtgaatcggg tgcgcgcttc gccgagtacc acgccagtca gtggttcacg
     8521 ggtctggtgc gagatcgcag gcgagtgccg attcctcctc agctcgcccc cgctgcgcag
     8581 ccgtgcgcac cgaaccgtgc gatgcgtttc gagttcgatg ccggccgcgg acgtccgacc
     8641 tttgccgaat tcgagacgcg tctgttcgac cagactgcgc tttgctattc gggcagcttc
     8701 ccgatgaccc gcgccatcga gatgcatgag cgtcctgacg gttcgcgcga catacgaaat
     8761 gtcggagcga tcgcctggcc ctcgggctgg ttgctgggtg gcggtctcgc aagcgacctg
     8821 ccggccctcg gcccgggcgc cagcagccag atcagcccgc aggcgggacg accgccgcgc
     8881 gaggccgcga tgcgcatggc cttgaggcgc acgccgtccg acgcctcggc cgcgctgtgg
     8941 gagctcgaac tcggtggcgt cgtggatgcc ccgatcgagt cgaagggatg gttgcacgtg
     9001 tcggtgccgc ggcgatgaaa tccgtgccgg atcctcgcct gtctgccttg gtcgggctgg
     9061 cactcgcggc caccgtggcc ttgtggtggc tgggcagcac ccgcatcgcc ctggaccagg
     9121 cgggcgatgc aagccgggcc gccgcagccg cgctcctggc gctctgggtg gtgcgcggca
     9181 tggtgctcgc gccgctgggc ttgcgcgccg gtgcgctgag cgggtggcgt gcgggggctg
     9241 cggcagcggc gttgctgctc gcgcccgcct ggccgttgtt gattcttgtc tggtcggcca
     9301 gcaccgtgcc cttgctgccg gccgcgctgg tggagttgtc gttgctgtcg gcaggtgtcg
     9361 tgctgccgtt gctgggtcag ggcctgcgtc gtgcactggg tcggcccgaa ctggccgaag
     9421 tcgtggccac ggccctcggc ctcgcgctgg cgtcgaccgc ctgggtcctg cgcgatctct
     9481 gggtctgggc accgccctga ggcgcgaatg gccgcgcttc cgcaaaccct gatcgaagcg
     9541 gtgcgacggc gcctgtggct cgggcaattc ctggctgcgg ctcgtctcgc actgtgggga
     9601 acggccggct tgatgctgct gaccgccgcg gtgcacctgg cgggctggcc gctgtcgcca
     9661 gacctcatgt ttctggcgct ggctttcgtg tggtcggtga tggcagcccg ggccgcatgg
     9721 caacgccctg tcgatgcagc ctgtgcgctc tgggccgacc gccatctggg cggggcctcg
     9781 gcatacacca ccctgctcga gctggactcg cccggaccgg gcaccccgcc atcgcacgcg
     9841 gtacgtcacc ttgaagcgtt cgctgcggcc caggtgccgc acagccttcg cctgctcgcc
     9901 cagcggcgtg atgcactgcg cctgtcccga ccgctgcttt tggcgctggt gtgtgcggcc
     9961 ttggcgtccc tggtgatgat gctgccgggt cccgatccaa agccgcagcc gccggcgaac
    10021 ctggctgcaa cttccggcct caccgatcgt gcggcgcctg acacggacgc gcccgtttca
    10081 gcggagctgg tcagcgagat ctccagtgcc ttgcgggcca cggcggccga tgcggcagag
    10141 gcgcctgagc gtcgcggtgc aggggacccg gcaacctcag gatccgacag ggccggcgat
    10201 gacggcgggt cggcagcggc cgcacccgat gccttggcgc agcgtccggc gcccgcggac
    10261 agtggctcgc ctgccgtcaa cccggggcaa gcggcagcgg cgcccggagc cagccgcctg
    10321 gctggatccg gttcggggca ggacgccggc gacagccgtg gcgagccggg cggcgtcggt
    10381 gttgcgcgca caccgcggag tacgattccg actcaggcag tcgagctggc ggcccgtcgc
    10441 ccgtcgggtg aaagacgggc tgatccggat caggcggcga cctacgatga ggagctctcg
    10501 atgcgaacgg cagcaagcgc ggcacggcgg gcggatgccg ctcccgccgc ggcgtcaccc
    10561 cccctggccg caccggagat gtggctgagc ccggccgaaa cgaattacgt gcaggcctgg
    10621 ttgaaggcca gtgcgagaca ccgatgaccg ccaacccgtt ctcggacagc caatacgctg
    10681 acgcgcatcg tcagctgcag gatttgcgca cgctgctcga ccaggccatc gtcggccagt
    10741 cggagctcgt cacccatctg gtcaccgcgg tgtttgcagg cgggcatgtg ctcatcgaag
    10801 ggctgcccgg tctgggcaag acgcatcttg ccaaggcgat tgcggccgcg ctggggctgc
    10861 gctgggcgcg cgtgcagtgc acgcccgacc tgatgcccgc cgatgtcacc ggcgcggaga
    10921 tctatgtcac cacggctggc ggggcgtcgt cgttcgagtt tcgacccggc ccggtattcg
    10981 cacagctcgt gctggtcgat gagatcaatc gcgcgacacc gcgcacgcag gccgcattgc
    11041 tcgaggccat gcaggagcgg caggtgacga atgccggggt ctctcaccgc ctgccggtcc
    11101 cgttctgcgt gctggccacg cagaacccga tcgaactcga aggcacctat ccgttgcccg
    11161 aggcccagct ggaccgtttc atgttcaagc tcacggtgcc gttcccgacg cgggaatccc
    11221 tgcggggcat gctcgatgtg tcgctcgacg ccgaaccttc cgacaccatg cagcccatcg
    11281 ccggccccga cgatgtgctg cggatctccg agctgtgccg cacgctgctg ctgggggagc
    11341 gctgcaagga agcggcggtc gatctgatcg tgtccacgca gcctgcggtc ggcggcgacc
    11401 cagccgacgc gcggcggcac atccggtacg gggccagccc gcgtgcactc cagtcgctgg
    11461 tcagggccgc gcgggtgcgc gcgctgatgg caggccgcac gcatgtcgac atcgatgatc
    11521 tcgcggcgat tgccctgccc gtgcttcgcc atcgggtgct cctgcggctg gagagcgagc
    11581 tcgacggcct ggaggtggac tccacgctcg catcgatcat cgacggatgg cgacttcggc
    11641 tctgatcccg gccgcgctgc ccagcgagcc cgaattgcgg tcgttcgcgt ccgctgcctc
    11701 gcagctgctg gtcgaacgcc agccgcgctc atccggttcg agggccgtct ctcgccgggc
    11761 cggggtcgga ctgcagcact tcgatcaccg cgactacgcg cccggggatg aagttcgcca
    11821 catcgattgg cggcagtcgg ctcgacgcag gcagccgatc gtgcgccgtt tcgaatcgga
    11881 atccgtcgcc gactggacga tcctgctcga cgcgagttca tccatggcgg tccagggtgc
    11941 ggcaaagtgg cgctcggcag tgcacgtcgc cgcggccatg agttacgccc tgcttcaaat
    12001 gggccatcgc gtgggattgc tggccttcgg ggcgcgcgtg ctgtcggaat gcccccgcgg
    12061 gcgcgggcag caccactacg ctgcgattgc ccggttgctg ctctcgctgc agccggcgcc
    12121 ggccggtgag cggtccgaac tgggtgtctg cacgggtcat ctgcgcggcg ccagcacggc
    12181 gttcgcgttg agcgacttcc tgtcatccga cgagatgcgt cgtgacctga gcgccatcgg
    12241 ccaacgctgc acggccctgc atgcgatgca gttgagcgac tcggccgaaa cacggctgcg
    12301 tccgttgggc gatgtcgatc tcgtggatgt ggaaacgggt gccagcatgc cggcgcatgc
    12361 cagcgagcac gctaatgcgc tggcagccga cgagcgtgct gcacggacgg cccggctgcg
    12421 cggcttctgt gcccgcagcg gcgtcgcgtt ctccgactgg gacatcgcaa atccctggca
    12481 gcacgcgctg ctgagacacc tcgtgcgggc gcgatccaat tgctgaacct ggtttcaccg
    12541 atgtggttgc tgggcctgct gctgctgccg gtgattcgct ggttgcatcg gggagggcgc
    12601 catcggcgcg ccatgccggt gtcgagcctc tctctctggg ccggggcggc cgcgagcgcg
    12661 cccgccgcgg gcgaacgcac gccgcctgat ccggcgtggc gacaccgtgc gttgctggcg
    12721 gcgttgatct tccttgcgct ggccgaaccg caactgccgg tgcagcgaac tcgcgtcacg
    12781 ctgtgggtcg atgactcgct cagcatgcag acccgcgaag cacgcggcac ccggatcagc
    12841 gaaggactgg cgcaggcccg ccgactgctg tccgagggcg ggtacaccga ggtggatgtc
    12901 cgggcgctcg gagacccgtg gcgcagcctc gggcccccgg gagatcgaac aggcgcgaac
    12961 ctctccgcga aggccggact caaggagccg acgccgcctc ccgcagcgct gcttcgcggc
    13021 gacaggctgc actggctgat caccgacggc accgatgcgg cgttgtcggg ctggcccgac
    13081 ggcaggcgcc ccgaccggat catcgaggtc gccggcacca gccgcaatgc cggactggag
    13141 cgtgtttccg cgcgccgcag cccgaacgat ccggacagga tcgacctgct gctcaaggtc
    13201 acgaatggag gcaacgaggc cgagacccgt gaggtggtgt tcatcacgga cgccggcgaa
    13261 tcggcacggt cgcggcaacg catcgagccg cagacctcga cgttcgtgac cgcgtcgatc
    13321 gcggaatcgt cccgggttcg cgtcaggctg gagcccggtg atgcgctggt cgaggacgac
    13381 acgatcgacc tcgacctcgc cccgttgcgc aagcgccggg tggccaccga ctcgacatgt
    13441 cccgggacgg tcgtcgcggc cgtggccagc cacccgggcc ttgccgtggc tgcggcggat
    13501 gccaccgacg ttcaggccgc tctcgattgc ggatcgcgag cctcggcccg cgatctggcc
    13561 acggtccgcg tcgtggccga tatcgttccg acatcattgc aggggccgtt gcagtggtcc
    13621 gcttcgctgc ctgagtcgag ccgcatcagc ctgtccatcg gatcactgca gacggcggca
    13681 cggctgcgcg ttcgccccgg ggatgccgtg ctgctggcgg ccgcagacga acccgtcatc
    13741 gtgatgggcc gctcggggcc ggccaagctg atcgagacat cgctggacct gggctcggag
    13801 gccgcgtcgc gcggcccgac ggttccgctc ctggtgaatc tgatgttcga gaccgtgatc
    13861 gggggcccgc tgctcgatcg gatcgcgatc ttggacaggg gcccggcctc ggtcttggtc
    13921 gtgccgtccg cgccagcgag tgccgccccg caggccccgc cggtgctgta cctcgcggcc
    13981 tcgggcggaa agccggcgtc gaaggcagct gcggttcatc gcgacggcgc gtggccgctg
    14041 ctggcggctg ccgtgctggt gctgctgtgg gaaatcgtct cgctgtgtcg acagtggatt
    14101 cgattgaacg gcagcacggg ggtggcgttc gagtgaccgg cgccggctcc aggacttcga
    14161 tcgcgctggc ccttcagctg gccgccctgg tggcgctgtc cttgtccctg ctcggggtcg
    14221 cgtggctcga tccgcgcagc aagccgcgcg cgctgctgct ggtcgatcgc tcccagagcg
    14281 tgccgcgcgc agccgccgac aaggccatcg ccgaagtcat gcaggcagcc cgggcaggcc
    14341 gcggtcgcga agtgcagctg ctcgagttcg cgggccgtct ggctgttccc gtggcgcagg
    14401 ccaacgatgc agtgaccgat ctcgagccgt cggtgacgaa catcgaggtg gcgctggatg
    14461 cggccttggc agcacatgcg caggaggcca tcgccagcgt cgtggtcatc tcagatggcc
    14521 tggagaacgt gggtgatgcg gcgcgtgcgc tgcgtgcggt gcgagaggcc cggttgccgc
    14581 tgcaatggat ccctgtgggg cgagaccctc cgcagacgcg catcgccgag gtcctggcgc
    14641 ctgcccgggc gctgcttgga caacggatcc cgatcacggt gcaactggcc ggtcagctgg
    14701 acccgccggt tcgggtcaag gccacgctgc gcacagccgg cggagacacg caggttgcga
    14761 gcagcctgtc gggaaaagcc ggcctggcaa cgatcgagtt cgacccgcgc agcagcggag
    14821 cggtcctcgt ggatgtggcg gtcgaggatg cggagtccgg acgcacgttc catgcaacgc
    14881 cggatgccgc cgtgatcgac gtggcacctc gcgcatccat cctctacgtg cagggatcga
    14941 gtggcgcgct tgcacacagc ttgcgccagg gaggctggtc gctcgatctc gttcctgccg
    15001 cccggctgga cgcccggacg gatgggctcg ccggatacca ggcggtcgtc ctggaggacg
    15061 tggccgtctc cgacgccggc ccgcgattct ggagcgccct ggtggatgcc gtgaagagcc
    15121 gcggtctggg cctgctggtg ctgggaggcg agcgttcgtt tgcccggggc ggctaccggg
    15181 gatcggttct cgaatcggtg ctgcccgtgc tgtccgagcc cgcggcgctg gacgaaccag
    15241 cggcgatcct gttcgcggtg gacaagtcgg gcagcatggg gcagggcagc ggcggcgtcg
    15301 atcgtcttgc actggcgcag cgtgccgtca tcgagaccgc gcgcggcctg acagctcgcg
    15361 attccctcgg tctgctggtc ttcgatgtcg agcctcggct gctgatcccg ctcggcccgt
    15421 cccaggccgg aatccgggcg ctcgagcgag attggcagtc gagtccgaac ggtggcacga
    15481 agctcgcgcc ggcactcgaa gcggccatcg ccgagctcga acggtcgcag gctgcgcgcc
    15541 gcatgctggt cgtcgtcacc gacggtttcg ttgacggcgc gcctgtcgac cagctgcgcg
    15601 cgcggcttga gcgctcgcgc atcgagctga ttgcactggc agtcggaccc gatgccgaca
    15661 tctccgccct cgagcgtgtg gcaggcgccg aagcgggcct ggtgctgcgc gtgaacgagg
    15721 ctgccgaact gccggtcgtc atgcgcgccg ggctcgagcg tcgccgggcg cggatcgagc
    15781 gcggcacgat cgccgcagag cagcatcagg cctttccctt cccgcccggc acatggaaag
    15841 actggcccgc cgtcgctgcc catgcggtca cccggccgca gccggacgcc gtggtggcgg
    15901 tgcgcagcca tcgcggtgag cccctgatcg cctttcacga atcgggtccg ggccgcgttg
    15961 cggccgtgac cagcgggctc gggccgtgga caccgcgctg gttgtcgtgg cgcgagtggc
    16021 cgcagttggc gggtggactg gcgacctggg tcagcggggc gccgcagggc gcgcagatcg
    16081 ggctgtcggt gtccgatctg ccgcaggacc tgcagatcga cgccgacctg cccgggcacg
    16141 caggtcgggc cgaatccgat gacttttcgg tggtggtcac cacgccggca tcgccgagcc
    16201 gttcgatcgc catggagcac gtgggaccgg gccggatgcg ggcgttgttg cccgaagccg
    16261 gccccggcct ctacacgttc gtgttgtcga ctccgctcgg aacccagcgc caactgcatc
    16321 tgcgccacca tcgcgccgag aacgagacct gggggatcaa tcctcggctg gcggcgtgga
    16381 cgaacgacgg gctggtcggt gcctggactc ccggcgagct ggcggcacct ccgcccagtg
    16441 atggcggcga ggatcgactt ccagtcgatc gcacgttgct cgcactggcg cttgcgctgt
    16501 tcctgtcggg cgtgctggtc gaccggactc gcctgccccg ggcgttcgtc agcgacaggc
    16561 tgcgtcgact gcgaacgcgc acctgacctt cagtgcgtca tgagtgctgc gccgtgccgg
    16621 cgatgtggcc cgccagcagg aaggatctgg cccaatcgcc gcgctggccg gcggtgatgg
    16681 tctcgtgcga tccgtccgcc atcacccggc cgtcgcgcat cagcagtacc cgggcgccca
    16741 gctcgatggc gtccgcagga tcgtgggtga tgatcatcgc gcccagaccg ttggccagcc
    16801 gggccttcag cctcgacacc aggtcgatgc gggtcaggcg gtccacattg ccgaacggtt
    16861 cgtcgagcag cagcagcttc gcgtcggccg ccagcgcacg ggccagagac acacgctggg
    16921 cctcaccgcc agacagtgtg gcgacatccc gaccgtccat ccctgccagg cccatgtcgc
    16981 gcagggctgc acgggccttc tcgaggcggc tggtcttgtc ggtcgcacgg atggcaaagg
    17041 cggcgttgcc cacggcgtcg aggtgcgaaa acaaggtggc gtcctggaac gccagcgcca
    17101 ggacacggtg ctccggcgcc accagcaccc ggccggcggc agacgcctcc cggccgtgca
    17161 ggtgcacgct gccagacgtc ggcgcgtcga agccggcgat catgcgcagc agcgaagtct
    17221 tgccgctgcc cgacggaccc aggacggcca cgatctcacc gggtgccatg tcgaaggaca
    17281 cctccgagac cacggcccgt tggccgatca gccgcgacaa agatcgaact gaaagcatgt
    17341 tgcgcacact ccgctgaact accgaattga cgagatcgag gccatcaggc ggcggccgtt
    17401 ctggcgccgg attccgaccg agggactccc agcctgtcca tgcgccacca gcacagcagc
    17461 agtggcagca gcatcaaggc ctgggatatc ag
//
LOCUS       DAOORV010000077        17386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_3987_length_17386_cov_7.595061, whole genome shotgun sequence.
ACCESSION   DAOORV010000077 DAOORV010000000
VERSION     DAOORV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_17386_cov_7.595061"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKV56_11260"
     CDS             <1..503
                     /locus_tag="PKV56_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOX68437.1"
                     /translation="VLEITENSLMKDLQSVIPSLHRLNEIGVEISIDDFGTGYSSLAY
                     LTTLPISELKIDRGFIRDLGMTPQSSAVVTAIIALARSLGLRVVAEGVENLRQMEVLH
                     RLGCGVMQGFLFSKPRPPSEIQSWLEETVLPRKAPWIGISPDEVSTEPSRSSMARPAR
                     TPNNAS"
     gene            518..2269
                     /locus_tag="PKV56_11265"
     CDS             518..2269
                     /locus_tag="PKV56_11265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX68438.1"
                     /translation="MANLPTASKGGGAATGAASEPASAADAQLPAQLAKAALRRLALD
                     KLEPTPENYARAYQQEQGKPPASGDSLADLIESIVRGIERGGKQWTLARKKESLQRVL
                     GGSRGDANRLQQRLRQLIGNWEADTNPDVKPDTPDETTSDAMPPSTSSEPASSTLPGP
                     ASASPGATLPAGPQGDPGEASGRVVTAFGQTVVRALPSADSGNRELAEAIAQASQRIR
                     SEGASEASAAEVEGLCERADWVLQHRDHLFEQISGLCLELTASLTDLAEDDSWAQGQC
                     EAMRISVEDGLTGRGVKSVSELLRTTRLKQAELRGERDAARDALKQMINRMLSELGEL
                     GSQTGRFHESVGRYADVIERADTLESLAGVVREMVEESRTVQGLVDQTQRRLSEEHAR
                     ATDLSERVTELESELRRLSSEVSTDQLTQIANRRGLLQAFEVECSRLERALADDASGS
                     ATEGLAIGLLDIDNFKRLNDELGHSAGDEALKALAATVSKTLRPTDMVARYGGEEFVV
                     LLPETPVDEGQQILTRLQRSLSGGLFMHEQKQIFVTFSAGITAYRTGERIEDALERAD
                     LALYQAKRAGKNRTLIG"
     gene            2310..3101
                     /locus_tag="PKV56_11270"
     CDS             2310..3101
                     /locus_tag="PKV56_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOX68439.1"
                     /translation="MITASSMSMTTYLALAGGFALLVLAIFWYRRRRNRRSHSTSTMG
                     PASLRQLRTGEFVRLVGQAFEARGFHVVESGVKGARAEGIVDIELRKDREAHLVHCKH
                     WRSRKIDVDAVREFHALMTERRAAGGFIVTTGRFSREAMHYVRGISLSLIDGSLLAPM
                     LDEGRYRKSAVTPSATMPLAAPKPVEWSPVSVMASVPDAMEDTYDLSRQTGGDTRPPP
                     IPAPPSCPLCSAPMLMRTAPEGKHAGRMFWRCSRRRECKGLRPLV"
     gene            3160..3588
                     /locus_tag="PKV56_11275"
     CDS             3160..3588
                     /locus_tag="PKV56_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylacetone-cleaving protein"
                     /protein_id="HOX68440.1"
                     /translation="MKSKTAEEYVQIDALAWKAFPAAFSNGGIRWKLLHVSPEQGAWT
                     AIFDCPAGSSFAPHVHAGPGEYLLTKGRMDVRGGKAAGGDTAVAPGYGYESSGARHDK
                     TFFPVDSEFYMTFLGPLTFIQPDGSPIAVVGWSEAQGAWG"
     gene            complement(3698..4390)
                     /locus_tag="PKV56_11280"
     CDS             complement(3698..4390)
                     /locus_tag="PKV56_11280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOX68441.1"
                     /translation="MGKRVTFDRPDGKGLEGYLAEPAHCDGAAAVVVVQEWWGVNDQI
                     RGVADRFAQAGYIALVPDLYRGQSTVEAEEAQHLMGGLDFGDAASQDIRGAVRFLKAR
                     SAKVGLTGFCMGGALTLLSMTKVPEVDAGVVWYGCPPLEFIDASAIRAPMLAHWATED
                     AFFPIATVDALEQKLQSAAVRYSGHRYLARHAFANETAVGPGRLAMTQYDPAWSQIAW
                     DRTFGFFGRWLG"
     gene            complement(4441..5541)
                     /locus_tag="PKV56_11285"
     CDS             complement(4441..5541)
                     /locus_tag="PKV56_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX68442.1"
                     /translation="MKRVVLVGAGHAHAQVLKDWCTSPPAGAELWVVSPHALAPYSGM
                     VPGWLAGLYRYEDICIDFAALTAAAGAHWVADELVALDPAHRRLTLGSGRQLDYDLLS
                     LNVGSTLRPPPSPAVPVLALRPLGELRLRWEAALATITRIAAVRPVTVTAVGGGAAGF
                     EALLAVRQRVRKMQPRSTVHLSLVSTGVELLPGMAPGAAGHARRTLTSLQATVQLGKR
                     FDEALPAATDLMLWATGAESHDWQRACGLAVSAPGWIRIDRHLRSISHPEVYATGDCA
                     AWEQPLPKAGVFAVRMGPVLSHNLCAALGTQAPVPYTPQRRVLSLLATGDRRAIASWG
                     RWSAEGAWVWRWKDRIDRGFIERFKVTPLAAN"
     gene            complement(5538..6152)
                     /locus_tag="PKV56_11290"
     CDS             complement(5538..6152)
                     /locus_tag="PKV56_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOX68443.1"
                     /translation="MNRPCPVIVMAKAPEPGYAKTRLIPALGRAGAAALADRLLAHAL
                     EQARVSALGPVDLCCAPDASHPAFQRHAASPVTQLSEQVDGDLGARMLHALERALTGH
                     DRALLIGTDAPALDAQRLREAADALDMHDAVFVPAHDGGYALVGLRYPMAMLFDGMTW
                     STPRVMDDTRARLRAHGLRHAELPPVHDIDEAADLAHLPAGWLA"
     gene            complement(6149..6826)
                     /locus_tag="PKV56_11295"
     CDS             complement(6149..6826)
                     /locus_tag="PKV56_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOX68444.1"
                     /translation="MARLLSIVIPTWNEARTIAEALDALAPLREAGAEVLVVDGGSQD
                     ATLALCAGRADRTLQAPCGRARQMNAGAAQARGEVLLFLHADTRLPADAMGSIAAVLA
                     GGADWGRFDVDIAGRSPMLPLIAALMNLRSRWSGIATGDQAIFVRRTLFESIGGFPDQ
                     PLMEDIELSSRLRRKHRPACLRARVVTSGRRWEQHGVWRTILLMWSLRLRYWLGTPAD
                     TLARAYR"
     gene            complement(6833..8953)
                     /locus_tag="PKV56_11300"
     CDS             complement(6833..8953)
                     /locus_tag="PKV56_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX68445.1"
                     /translation="MALLAAVLYRHFDIGDLLTLDSLKASRDTLVARFDQSPATFAIG
                     YFALYVAVAALSLPGAAVMTLAAGAIFGLGLGLLLVSFASSIGATLAFLTARYLLRDS
                     VQARFGSRLEPVNEGMRRDGAWYLLSLRLVPVFPFFMVNLLVGLTPIAAVRFYWVSQL
                     GMLAGTAVYVNAGTQLAGITNLADVLSPPLLLSFVLLGVFPLLAKALLGALQRRKVYA
                     NWPRPKRFDRNLIVIGAGAGGLVTAYIAAAVRARTTLVEDHKMGGDCLNYGCVPSKAL
                     IRVAKLARDIQQADAMGIRDASGRIDFAAVMARVQRVIRDIEPHDSVERYTALGVDVV
                     QARARITSPWSVELTRADGTVQTLTTRSIVIATGARPFVPPIPGLQQAGFLTSDTLWG
                     LAELPRRLLVLGGGPIGCELAQCFARLGSEVTQVEMAPHVLGREDEDASAVVAQALRD
                     DGIELLTGHEAVRVETIDGEHRLIVRQGQVERAVAFDRLLCAVGRSPRVAGYGLEELG
                     IPLSTVKTIETNAWLETLYPNIYAVGDVAGPYQLTHVAAHQAWYAAVNALFGLFKRFR
                     VDYSVIPRATFTDPEVARVGLSEAEANAQGVSVEVTRFPLADLDRAIADGTTRGFIKV
                     LTLPGRDRIVGVTIVGAHAGDLLAEYALAMKHGLGLNKILGTIHSYPTLAEANKYVAG
                     AWKRAHAPQRLLAWVERYHAWQRG"
     gene            complement(8998..9972)
                     /gene="arsS"
                     /locus_tag="PKV56_11305"
     CDS             complement(8998..9972)
                     /gene="arsS"
                     /locus_tag="PKV56_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463910.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HOX68446.1"
                     /translation="MHPTFPLLARTDFPRLRRRSLETLQVNLGYRCNQSCLHCHVNAG
                     PTRTEAMDADTADLVLEVLERRGVKTLDLTGGAPEMNPQFRRLVQATRAMGVRVIDRC
                     NLTILFEPGHEDLADFLAAQQVEVVASLPCYSVANVDRQRGEGVFDKSIAALQKFNAL
                     GYAQPGSALTLNLVYNPQGPVLPPPQEALQADYKRELEAHFGIRFNHLFALTNMPIQR
                     FGSTLVSKGQFESYLQLLKDAYRADNLDTVMCVSQVSVDWQGDLYDCDFNQMLGLPAR
                     LDGRTRAHLRDLLEHSPVDEIIRVADHCHGCTAGQGSSCSGALEVGSR"
     gene            complement(10002..10298)
                     /locus_tag="PKV56_11310"
     CDS             complement(10002..10298)
                     /locus_tag="PKV56_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68447.1"
                     /translation="MKRILVIALALGLCACDAVDEAVAAGNHMNHILEKCVQHMKEKQ
                     GDTDIPECNGMTEQAEYLKAKTKVKEPSAALDSIDRRSMQLRQEAIGLMQGSAR"
     gene            complement(10368..11123)
                     /locus_tag="PKV56_11315"
     CDS             complement(10368..11123)
                     /locus_tag="PKV56_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOX68448.1"
                     /translation="MTDIDIAVSRLVRARHEHQPQPAPALADATAAYAAQERVARELA
                     WFGTAPPRYWKSGGASRAAVISHAPLPPAGVWISPAQAGTWPEVRGGIEAEVALRLRE
                     PVDAERAATLDLSGARKLIDAMCVSIEIVDSRWAEGTQAPALAQLADVLSHGALVLGA
                     WVGFEASREWSTQFCRVRIGSRPERVFQGTHPLADPVFVVPAWLRHATRHGQELPAGS
                     IVTTGSWCGLLQAQAGDRVEVAFDGMGEACVQL"
     gene            11227..11691
                     /locus_tag="PKV56_11320"
     CDS             11227..11691
                     /locus_tag="PKV56_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOX68449.1"
                     /translation="MRRFIPILALACATAAQAESIGAVDTVFKFIGPDHKIVVEAYDD
                     PKVNGVTCYLSRAKTGGIKGALGLAEDRAEASIACRQIGPISIAQALPEQEEIFNERI
                     SLVFKKLRIVRMVDAKRNALVYLTYSDRLIEGSPQNSVTVVPVDRSAAIPVK"
     gene            complement(11807..12439)
                     /locus_tag="PKV56_11325"
     CDS             complement(11807..12439)
                     /locus_tag="PKV56_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX68450.1"
                     /translation="MKKLIIAMLMAAPFFPAAANLVTNGSFEATAQGSGSWSIYPSVS
                     GWTGIGHGIEVRNNVAGSAIDGHNFVELDTTRNSSMAQTLTGDAGTYLLSFWYQTRPN
                     TSKATNGLSFSLDGVDLGSVLLSSDAAALDYNWHQYTSLVDFDGSGDLRFNAIGTSDS
                     YGSSLDNIEFTAIVPELQIAAAVPEPGTTALVLAALAAAGLASRRRKIQS"
     gene            complement(12683..13453)
                     /locus_tag="PKV56_11330"
     CDS             complement(12683..13453)
                     /locus_tag="PKV56_11330"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOX68451.1"
                     /translation="MPHAVSRLRAARLARSAKPFLARGGFKRERCAGCRLLPSHCMCA
                     LRPAVPTRSGVCLLMADIEPLKPSNTGWLIADVVADTFAFGWARTETDPDLLSLLSDP
                     QWQPYVVFPGEYVAPERVVHSVQTADTLEGGSGKRPLFVLLDATWSEARKMFRKSPYL
                     DHLPVLSLNPDQLSQYKLRRSHRGDHFCTSEVAALCLKLAGESLAEQTLEAYLEVFTL
                     HYLQAKNQLPIAWDDAAHRRLHELSLSALLPLRDAAEA"
     gene            13671..14012
                     /locus_tag="PKV56_11335"
     CDS             13671..14012
                     /locus_tag="PKV56_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOX68452.1"
                     /translation="MSALPNCPQCNSELTYEDGSQFVCPECGHEWSGEAATEVAEALR
                     VVRDAHGTELKDGDTVTVIKDLKIKGSSLVVKVGTRVKNIRLAEGDHDIDCKIDGIGA
                     MGLKSEFVKKA"
     gene            14257..14874
                     /locus_tag="PKV56_11340"
     CDS             14257..14874
                     /locus_tag="PKV56_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68453.1"
                     /translation="MGEFWKKVEILRWDDSRYYHQCRINQTLHLASAISFVAAYVLMF
                     VDPASAALVGWLVGMITRQSGHIFFEPRGFDSIHGVSYQQKESIKVGYNMRRKSVLIG
                     VWLCLPALLLASPSLFGLIESAAGWQGYARDVGLSWLALGIGGLLFRTVHLFFLRGPA
                     WGLAWAVKILTDPFHNIAIYWKSPLALLQGQRFDPMGTHRHPAET"
     gene            15520..16152
                     /locus_tag="PKV56_11345"
     CDS             15520..16152
                     /locus_tag="PKV56_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOX68454.1"
                     /translation="MNKTTGGELAAVLKAETGALHSLTERSGAMADLLRGRLSLHDYC
                     VMLRNLQPIYAVLEAGLDEHAEDARLWRPELRRLHALEQDLDQLHAGDWRSELIVAPT
                     AGLYVTRLTALIHKEPLLLLAHAYVRYLGDLSGGQMLSGLIRRQYGLDCQAGTAFYAF
                     GEPSHVDALKRDFRDGLDALTLMPTQRAAFVEEAREAFRLHQRLFEELRA"
     gene            complement(16202..17329)
                     /locus_tag="PKV56_11350"
     CDS             complement(16202..17329)
                     /locus_tag="PKV56_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68455.1"
                     /translation="MEATSIEGDWRYALFRNPRTLLQPLEATGAALFCEGEVLTAGET
                     PSTPELRALLAWVQDQPQPSDAPFSCSSIAQKNPALSSLTPTASGVLAVPLSNNSPDW
                     LIWFRKEQLQSVTWAGEPSKSMVANNPLELEPRRSFAAWSEIVRGTAVPWTPTELALA
                     RGIGIALVDIIAQVHAVRLLIAEHQLVQIRGTVAGSREPVLVANAQGRAVFANDALVA
                     VRGSAVDREVPIEALFTNSPAISQALEQMRTSKHGWRRELDLSTGSGSAVPMDVHAEP
                     VSGRDGKLLGFVLRLVDLREARRADTARLNLETALRLAAVNSPAEADEVISAILTNAS
                     LAAMDIADARGGVPVAPLLQELEDSTRRATGLYVQIRSFFS"
BASE COUNT         2962 a         5674 c         5815 g         2935 t
ORIGIN      
        1 tggtgctcga gatcaccgag aactcgctga tgaaggacct gcaaagcgtc atcccttcgc
       61 tgcaccggct caacgagatc ggtgtcgaga tctcgatcga tgatttcggc acaggctatt
      121 catcgctggc gtacctgacg accttgccga tcagcgagtt gaagatcgac cgcggtttca
      181 tccgcgacct gggcatgacg ccccagagct cggcggtggt caccgcgatc attgcgctgg
      241 cgcgctcgct cggcctgcgc gtggtcgccg aaggtgtcga gaacctgcgt cagatggagg
      301 tgttgcaccg actgggatgc ggcgtgatgc agggcttcct gttcagcaag cctcggccgc
      361 cttcggaaat ccagtcctgg ctcgaggaaa ccgtgcttcc cagaaaggca ccttggatcg
      421 gtatctcgcc cgacgaagtc tccaccgagc cttcgcgctc cagcatggca cgtcctgcgc
      481 gcaccccgaa caatgcaagt tgagatccga ctgaaagatg gctaacctgc cgactgcttc
      541 caaaggaggc ggtgccgcca ccggagcggc ttccgagcca gcatcggctg ccgacgcaca
      601 acttcccgcc cagctggcca aggctgccct gcgtcggctg gctctcgaca agctcgaacc
      661 gacccccgaa aactacgccc gtgcctacca gcaggagcag ggcaagccgc ccgccagcgg
      721 cgattcgctt gccgacctga tcgaaagcat cgtgcgtggc atcgagcgtg gcggcaagca
      781 gtggacgctg gcacgcaaga aggaaagcct gcagcgcgtg ctcggcggca gccgtggcga
      841 tgccaatcga ctccagcagc ggttgcgcca actgatcggc aactgggagg ccgacaccaa
      901 ccccgacgtc aagcccgaca cgcccgacga aacgaccagc gacgcgatgc cgccctcgac
      961 gtcgtccgag cccgcttcga gcaccctgcc gggtcccgca tcggcttcgc ccggagcgac
     1021 gctgccggcg ggtccgcagg gcgatccggg ggaggcctca ggtcgtgtcg tcacagcgtt
     1081 cggccagacc gtggtgcgtg cgctgccttc ggctgattcg ggcaaccgtg aactcgccga
     1141 agccatcgcg caggcgtccc agcgaattcg ttcggaaggc gccagcgagg cgagcgccgc
     1201 ggaagtcgaa ggactctgcg aacgtgccga ctgggtgctg cagcaccgcg atcacctgtt
     1261 cgagcagatc agcggcctgt gcctggaatt gaccgccagc ctcaccgatc tggccgagga
     1321 cgacagctgg gcgcagggcc agtgcgaggc gatgcgcatc tccgtcgagg atggcctgac
     1381 cggtcgtggc gtcaagtctg tcagcgagct gctgcggacg acacgactga agcaggcgga
     1441 actgcgcggc gaacgcgatg cagcccgtga tgcgctcaag cagatgatca accgcatgct
     1501 cagcgaactc ggtgagctgg gttcgcagac gggtcgcttt catgaaagcg tcggacgcta
     1561 cgccgatgtc atcgagcgag cggacacgct cgaaagcctg gccggtgtgg tgcgagagat
     1621 ggtcgaggaa agccgcacgg tgcagggtct ggtcgaccag acccagcggc gcttgagcga
     1681 ggagcacgcc cgggccaccg acctgtccga gcgcgtgacc gaactggaat ccgaactcag
     1741 gcgcctgtcg agcgaggtgt ccaccgacca gttgacgcag atagcgaatc gtcgcggcct
     1801 gttgcaggcg ttcgaggtcg aatgctcgcg cctggaacgc gcgctggccg acgatgcgtc
     1861 cggcagcgcc acggaggggc tggcgatcgg gttgctcgac atcgacaact tcaagcgcct
     1921 gaacgacgaa ttgggacaca gtgcaggcga cgaggccttg aaggcgctgg ccgccaccgt
     1981 cagcaagacg ctgcgtccca ccgatatggt ggctcgctat ggcggcgagg aattcgtcgt
     2041 gctgctgcct gagaccccgg tcgacgaggg ccagcagatc ctcacgcgat tgcagcgttc
     2101 gctgagcggc gggctgttca tgcacgagca gaagcagatc ttcgtcacct tctccgcggg
     2161 catcacggcc taccgtacgg gtgagcggat cgaagatgcg ctcgagcggg cggacctggc
     2221 gctctaccag gccaagcgcg ccggcaagaa ccggacgctg atcggctgac cacgacgatg
     2281 gtctggatgg atcgcctcgt cagcgtcgca tgatcaccgc atccagcatg tcgatgacca
     2341 cctacctggc gctggcgggc ggcttcgcgc tgctggtgct ggcgatcttc tggtaccgac
     2401 gccgccggaa tcggcgctcc cattccacct cgacgatggg ccccgcctcg ttgcgccagt
     2461 tgcgtaccgg cgagttcgtg cgtctcgtcg gtcaggcttt cgaggcccgg ggcttccacg
     2521 tggtcgagtc cggcgtgaaa ggcgcgcgtg ccgagggcat cgtcgacatc gaactgcgca
     2581 aggaccggga ggcccacctg gtgcattgca agcactggcg atcacgcaag atcgatgtcg
     2641 atgcggtgcg tgaattccat gcgctgatga ccgagcgccg cgctgccggc ggattcatcg
     2701 tgacgaccgg ccgcttcagc cgcgaggcga tgcactatgt gcgaggtatc agcctcagcc
     2761 tcatcgatgg gtcgttgctg gcgccgatgc tcgatgaggg gcgctatcgc aagtcagccg
     2821 tgaccccgtc cgccacgatg ccgctggccg cgcccaagcc ggtggagtgg tcgccggtgt
     2881 ccgtgatggc ctcggtcccc gacgcgatgg aggacaccta cgatctgtct cgacagacgg
     2941 gaggcgacac gcgcccgccg cccatcccgg cgccgccgag ctgcccgctg tgttccgcac
     3001 cgatgttgat gcgtaccgct cccgagggca agcatgccgg ccgcatgttc tggcgttgct
     3061 cgcgccgccg cgaatgcaag ggcttgcgac ccctggtcta gagtgcagcg cggtccaccg
     3121 ggccgatcga tccgccagtt cccatcgaag gagacagcca tgaaatccaa gaccgcagaa
     3181 gagtacgtgc agatcgatgc gctggcctgg aaagcctttc cggcagcatt ctcgaacggc
     3241 ggaatccgct ggaagctgct tcatgtgtcg cccgagcagg gcgcgtggac cgcgatcttc
     3301 gattgcccgg cgggctcgtc gttcgcgccg catgtccatg ccggaccggg cgagtacctg
     3361 ctgaccaagg ggcgcatgga tgtgcgcggc ggcaaggcgg ctggtggcga cacggcggtc
     3421 gctcccggat acggctacga gtcgtccggc gcccgccacg acaagacatt cttcccggtc
     3481 gacagcgagt tctacatgac cttcctgggt ccgctgacct tcatccagcc cgatggctcg
     3541 ccgatcgcgg tggtcggctg gtccgaagcc cagggcgcct ggggctgagc acgcgcgcca
     3601 ggcagcgcct gcgcgccgtc tggcggcagg cggggtctgc cttggctgcc tgcttgccgg
     3661 tcgagtgctg ccgttgaggc cgagttcgca accggactca gcccagccac cgaccgaaga
     3721 atccgaaagt ccggtcccac gcgatctgcg accacgccgg gtcgtactgc gtcatggcca
     3781 gtcggcccgg cccgacggca gtttcgttgg cgaaggcgtg ccttgccagg tagcggtgac
     3841 cgctgtagcg cacagcggcg gactgcagct tctgctcaag cgcgtcgacg gtggcaatgg
     3901 ggaagaacgc gtcttcggtt gcccagtgcg cgagcatcgg cgcacggatg gccgaggcat
     3961 cgatgaactc gagcggaggg cagccgtacc agaccacacc ggcatcgacc tcgggcacct
     4021 tcgtcatcga cagcaaggtc agcgcgccgc ccatgcagaa gccggtcagg ccgaccttcg
     4081 ccgaacgcgc tttcaggaat cggaccgcac cccggatgtc ctgcgaggcg gcgtcgccga
     4141 aatcgaggcc gcccatcagg tgctgggctt cttcggcctc gactgtggac tggccgcggt
     4201 acagatcggg caccagcgcg atgtagccgg cttgcgcgaa acgatcggcg acaccgcgaa
     4261 tctgatcgtt gacgccccac cactcctgca ccacgaccac tgcagccgcg ccgtcgcaat
     4321 gcgccggttc ggcgaggtag ccctcgaggc ctttgccatc cggacggtcg aacgtgacgc
     4381 gttttcccat ggcggtctcc tgtgtgttct gacaacggcg cagaggataa atgctgcgct
     4441 tcaattcgcc gcaagtggcg taaccttgaa gcgctcgatg aacccgcggt cgatccgatc
     4501 cttccagcgc cagacccagg cgccttctgc ggaccagcgt ccccatgatg cgatggcgcg
     4561 gcggtcgccg gtggccagca atgacagcac ccggcgctgg ggggtgtagg ggacgggcgc
     4621 ctgtgtgccg agggctgcgc acaggttgtg cgacagcacc ggccccatgc gtaccgcaaa
     4681 cacgcctgcc ttcggcagcg gctgttccca ggcggcgcag tcgcccgtgg cgtagacctc
     4741 ggggtgcgaa atcgagcgca ggtggcggtc gatgcgtatc cagccgggcg cgctgaccgc
     4801 caggccgcag gccctttgcc agtcgtgcga ttcggccccg gtcgcccaca gcatcaggtc
     4861 ggtggccgct ggcagcgctt cgtcgaatcg cttgcccagt tgcaccgtgg cctgcagcga
     4921 ggtcagtgtc cgccgcgcat gccctgccgc acccggggcc atccccggca gcagttcgac
     4981 gccggtgctc accagcgaaa gatgcaccgt gcttcgaggc tgcatcttcc tcacccgctg
     5041 ccgcaccgcc agcagggcct cgaagcctgc cgctccgccg ccgactgccg tgaccgtcac
     5101 gggtcgcacg gcggcaatgc gggtgatcgt cgccagcgcg gcctcccacc gcagccgcag
     5161 ttcaccgagt ggacgcagcg ccagcaccgg gacagcgggc gatggcggtg gcctgagggt
     5221 cgagccgacg ttgagcgaca gcaggtcgta atcgagctgc cgaccgctgc cgagggtcag
     5281 ccggcgatgt gccggatcga gcgccaccag ttcgtcagcg acccagtgcg cgccggcggc
     5341 tgccgtcagc gcagcgaagt cgatgcagat gtcctcgtag cgataaaggc ctgccagcca
     5401 gcccgggacc atgcctgaat agggcgccag cgcgtgcggt gacaccaccc acagctcggc
     5461 cccggccggc ggcgacgtac accaatcctt cagcacctgc gcgtgagcgt ggccggcacc
     5521 gaccagcacg acccgcttca tgcgagccag cccgctggca ggtgcgcgag atcggcggcc
     5581 tcgtcgatgt cgtggacggg gggcagctcg gcgtgccgca ggccgtgcgc gcgcagccgg
     5641 gcgcgcgtgt cgtccatgac ccggggcgtg ctccaggtca tgccgtcgaa cagcatcgcc
     5701 atcgggtacc tcaggccgac cagcgcatag ccgccgtcgt gcgccggcac gaagaccgcg
     5761 tcgtgcatgt cgagcgcatc ggcagcctcg cgcaatcgtt gcgcgtcgag ggcaggcgcg
     5821 tcggtgccga tcagcagtgc gcgatcgtgg ccggtcagtg cgcgctccag cgcatggagc
     5881 atgcgggcgc ccagatcgcc atcgacctgc tccgaaagct gggtcacggg tgacgcggcg
     5941 tggcgctgga aggcagggtg cgatgcgtcg ggcgcacagc acagatccac cggtccgagc
     6001 gcgctgaccc gggcctgttc gagcgcatgt gccagcagcc ggtcggccag cgcagccgcg
     6061 cccgcacgtc ccagggcagg aatcagtcgg gtcttggcgt agccgggttc aggcgccttg
     6121 gccatcacga tcaccgggca aggcctgttc atcggtaggc gcgggcgagc gtgtcggccg
     6181 gcgttccgag ccagtagcgc agacgcagcg accacatcag caggatcgtt cgccacacgc
     6241 cgtgctgttc ccagcgacga cccgaggtga cgacgcgtgc gcgcaggcaa gccggacggt
     6301 gcttgcggcg caggcggctc gacagctcga tgtcttccat cagcggctgg tcggggaagc
     6361 cgccgatcga ctcgaacagc gtgcggcgca cgaagatcgc ctgatcgccg gtggcgatgc
     6421 cgctccagcg cgagcgaagg ttcatcagcg cagcgatcag gggcagcatc ggcgagcggc
     6481 cggcgatgtc gacatcgaag cggccccagt cggcgccgcc ggccagcacc gccgcgatgg
     6541 atcccatcgc atctgcaggc agtctggtgt cggcatgcag gaacagcaac acttcgccgc
     6601 gcgcctgcgc tgctcccgcg ttcatctgtc gcgcgcgacc gcacggcgcc tgcagtgtcc
     6661 gatcggcccg acccgcgcaa agcgccagcg tagcgtcctg gctgccgcca tcgacgacca
     6721 gcacctcggc acctgcttct cgcaggggtg ccagcgcatc gagtgcttcg gcgatggtcc
     6781 gtgcctcgtt ccaggtcggg atgacgatgg acaggaggcg cgccacggtg aatcagcctc
     6841 gttgccaggc gtgatagcgc tcgacccagg ccagcagtcg ctgcggcgcg tgggcgcgct
     6901 tccaggcacc ggcgacgtac ttgttggcct cggccagggt cgggtagctg tggatcgtgc
     6961 cgaggatctt gttgagtccc agtccgtgct tcattgcgag tgcgtactcg gccagcaggt
     7021 ccccggcatg tgcaccgacg atcgtcactc cgacgatcct gtccctgccg ggcaacgtca
     7081 gcaccttgat gaacccccgg gtggtgccat cggcgatggc gcggtcgaga tccgcgagcg
     7141 ggaagcgcgt cacctcgacc gacacgccct gagcattcgc ttcggcttcc gacaatccga
     7201 cgcgtgccac ctcgggatcg gtgaaggtgg cccgcgggat caccgagtag tcgacccgaa
     7261 agcgcttgaa gagtccgaac agcgcgttca ccgccgcgta ccaggcctga tgcgccgcga
     7321 catgcgtgag ctgataaggc cccgcgacat caccgaccgc gtagatgttc gggtagagcg
     7381 tttcaagcca ggcgttggtc tcgatcgtct tcacggtgga cagtgggatg cccaactcct
     7441 cgagcccgta gcccgcgacg cgcgggctgc ggccgaccgc gcagagcagc cgatcgaagg
     7501 ccaccgcgcg ctcgacctgg ccctgcctga cgatcagcct gtgttctcca tcgatggttt
     7561 ccacgcgcac ggcttcgtga ccggtcagca gttcgatgcc gtcgtcgcgc agcgcctggg
     7621 cgacaacggc cgatgcgtcc tcgtcctcgc ggccgagcac gtgcggggcc atctcgactt
     7681 gcgtgacctc ggagcccagc ctcgcaaagc actgcgccag ttcgcagccg atcggaccac
     7741 cgcccagcac cagaagacgg cgaggcaact cggccagacc ccacagcgtg tcgctggtga
     7801 ggaagcccgc ctgttgcagg ccgggtatcg gcggcacgaa cgggcgggcg cccgtggcaa
     7861 tgacgatgct gcgcgtggtc agcgtctgca ccgtgccatc ggcacgcgtc agctccacgc
     7921 tccatggcga ggtgattcgc gcgcgtgcct gcaccacgtc gacgcccagt gcggtgtagc
     7981 gctcgacgga gtcgtgtggc tcgatgtcgc gtatgacgcg ctgcacccgt gccatcacgg
     8041 ccgcgaaatc gatgcggccg ctcgcatcgc gaatgcccat cgcgtccgcc tgctggatgt
     8101 cccgggccag cttggccact cggatcagtg ccttgctcgg cacgcatccg tagttcaggc
     8161 agtcgccacc cattttgtgg tcttccacca gcgtcgtgcg ggcccggacc gcggcagcga
     8221 tgtaggccgt caccaacccg cccgcaccgg ctccgatgac gatcagattg cggtcgaagc
     8281 gcttcggtcg tggccaattc gcgtagacct tgcgccgctg cagcgcgccg agcaacgcct
     8341 tggcgaggag cgggaatacg cccagcagga cgaagctaag cagcagtggc ggcgacagca
     8401 catccgccag attcgtgatg cccgcgagtt gggtgccggc gttcacgtac acggcggtgc
     8461 cggccagcat gcccagctgg ctgacccagt agaagcgcac cgcagcgatc ggcgtcaggc
     8521 cgaccagcag gttgaccatg aagaacggaa agaccggcac cagccgcagg ctcagcaggt
     8581 accacgcacc gtcacgtcgc atgccttcgt tgaccggttc gagccgcgag ccgaagcggg
     8641 cctgaaccga gtcgcgcagc aggtagcggg ccgtcaggaa ggccagcgtc gcaccgatgc
     8701 tcgacgcgaa cgacaccagc agcagaccga ggcccaggcc gaagatggcg ccggctgcca
     8761 gcgtcatcac cgccgcgcct ggcagcgaca gcgcagccac ggcgacgtag agggcgaagt
     8821 agccgatggc gaacgtggcg ggtgactggt cgaagcgggc gaccagcgtg tcgcggctgg
     8881 ccttgagcga gtcgagcgtg agcaggtcgc cgatatcgaa gtgccggtac agcacggcag
     8941 cgagcaaggc catcagcgcg acagcgatcc acagaccgcg atggcgcgac gaagtcgtca
     9001 ccgggatccc acctcgagtg cgccgctgca gctgctgccc tggccggcgg tgcagccgtg
     9061 gcaatggtct gccacgcgga tgatctcgtc gaccgggctg tgctccagca ggtcgcgcag
     9121 atgggcccgc gtgcggccgt cgagccgcgc cggcaggccg agcatctggt tgaaatcgca
     9181 gtcgtagagg tcgccctgcc agtcgacgct gacctgcgac acgcacatca ccgtgtccag
     9241 gttgtccgcc cggtaagcat ccttgagcaa ctggaggtag gactcgaact gccccttcga
     9301 caccagcgtg ctgccgaacc gctggatcgg catgttggtg agcgcgaaca ggtggttgaa
     9361 gcggatgccg aaatgagctt cgagctcgcg cttgtagtcg gcctgcagcg cttcctgtgg
     9421 cggcggcagc accggcccct gcgggttgta gacgaggttc agcgtcagcg cgctgccggg
     9481 ctgtgcataa cccagtgcat tgaatttctg cagtgcggcg atgctcttgt cgaacacgcc
     9541 ttcgccgcgc tggcggtcga cgttggcgac cgagtagcac ggcagcgacg cgaccacttc
     9601 cacctgctga gcggccagga aatccgcgag gtcctcgtgc cccggctcga acaggatggt
     9661 caggttgcag cggtcgatca cgcgcacccc catcgcgcgc gtggcttgca ccagccgacg
     9721 aaactgcggg ttcatttcgg gggcgccgcc ggtcaggtcc agcgtcttca caccgcgccg
     9781 ttcgaggact tccagaacca ggtccgcagt gtctgcatcc atcgcctcgg tccgtgtcgg
     9841 gccggcgttc acgtggcagt gcaggcagct ctggttgcag cggtagccga ggttgacctg
     9901 cagtgtttcg agggatcgac ggcgcagccg cgggaagtca gttcgggcca gcagcgggaa
     9961 cgtgggatgc atgggcgcgc ctcagccgtc gcttcggccg gctaccgggc cgaaccctgc
    10021 atcaggccga tggcttcctg gcgcagttgc atcgaccgcc gatcgatgga gtccagcgcg
    10081 gcgctgggct ccttgacctt cgtcttcgcc ttgaggtatt ccgcctgctc ggtcatgcca
    10141 ttgcattccg gtatgtcggt atccccctgc ttttctttca tgtgctgcac gcacttctcg
    10201 aggatgtggt tcatgtggtt tcccgcggcg accgcctcgt cgacggcatc acaggcgcac
    10261 aggcccagcg cgagggcaat gacgagaatt cttttcatga tgggtcgggc ttcgttggct
    10321 ccaggtctga ttgtgtcgaa cgcggcagcg cggtcggttc gaaggggtta cagctgcacg
    10381 caggcttcac ccattccgtc gaaggccact tcgaccctgt cgcccgcctg tgcctgcaac
    10441 aagccgcacc agctgccggt ggtcacgatg ctgccggccg gcaactcctg gccgtgacgg
    10501 gtggcgtgac gcagccaggc cggcaccacg aagaccgggt ccgccagtgg gtgggtgccc
    10561 tggaatacgc gttccgggcg cgagccaatg cgcacccggc agaactgtgt cgaccactcg
    10621 cgcgacgctt cgaatccaac ccaggcgccg agcaccagcg cgccatgcga cagcacatcg
    10681 gccagttggg cgagtgcggg cgcctgcgtc ccttcggccc atcgcgaatc gacgatctcg
    10741 atcgatacgc acatcgcatc gatcagtttc cgtgcccccg acaggtccag cgtcgccgcc
    10801 cgctctgcat ccaccggttc ccgcagtcgc agcgcgacct cggcctcgat gccccctcgc
    10861 acctcgggcc aggttccggc ctgtgcggga ctgatccaga caccggcggg tggcaggggc
    10921 gcgtgggaga tgacagccgc gcgtgacgcg ccgccggact tccagtacct cggtggcgca
    10981 gttccaaacc aggccagctc gcgggccaca cgctcctgcg ccgcataggc cgcagtcgcg
    11041 tcggccagcg ctggcgccgg ctgcggttga tgctcgtggc gggcccgcac gagtcgcgag
    11101 acggcaatgt cgatgtctgt catggcacct ggggtcggtt tcggccgcgt cgggcagccg
    11161 cgtgcatcat cgcagcgtgc agacaacggc ccgcactccg cgggccgtgc gacgaaagga
    11221 cccacgatgc gacgcttcat tccaattctg gccctggcct gcgccacggc cgcgcaggcc
    11281 gagtccatcg gcgcggtcga caccgtcttc aagttcatcg ggccggacca caagatcgtg
    11341 gtggaagcct acgacgaccc gaaagtcaac ggcgtcacct gctatctgtc gcgcgccaag
    11401 acggggggga tcaagggggc gctcggactc gcggaggatc gggccgaagc gtcgatcgcc
    11461 tgcaggcaga tcgggccgat ctccattgcg caggcgctgc ctgaacagga ggagatattc
    11521 aacgagcgga tcagcctggt cttcaagaag ctgcgcatcg ttcgcatggt cgacgccaag
    11581 cggaatgcgc tggtgtacct gacctactcc gacaggctga tcgaggggtc gccgcagaac
    11641 tctgtcacgg tcgtccccgt cgatcgatca gcagccattc ccgtcaagtg acatggcgct
    11701 tcgaacgagc cgccggcacc ctgggagcct atgcggtggt tcgaaccgtc gatggtcgga
    11761 aaaaagccgc ccgaaggcgg ctctcgtcat gtcagacccg aacaggtcag gattggatct
    11821 tgcggcgacg cgaagccagg ccggctgcag ccaacgccgc caacaccagt gcagtggtac
    11881 cgggttcagg aacggctgcc gcgatctgca gttcaggaac gatggctgtg aactcgatgt
    11941 tgtccagtga gctgccgtag gaatcgctgg ttccgatcgc gttgaagcgg agatctcccg
    12001 aaccatcgaa gtcgacgaga ctggtgtact ggtgccagtt gtagtcgagg gctgcggcgt
    12061 cggagctcag cagcacggat cccaggtcga ctccatcgag agaaaacgac agaccgttgg
    12121 ttgccttgct ggtattcggc cgggtctggt accagaagct gagcaggtat gtgccggcat
    12181 cgccggtcag cgtttgtgcc atcgagctgt tgcgcgtggt gtcgagttcg acgaagttgt
    12241 gcccgtcgat ggcgctgccg gcgacgttgt tgcggacttc aatgccgtgg ccgattcccg
    12301 tccagccgga gaccgaggga tagatgctcc agctgccgga cccttgggcg gtggcttcga
    12361 agctgccgtt ggtcacgagg ttggctgcag cgggaaagaa cggtgcggcc atcagcatgg
    12421 cgatgatcaa cttcttcatg gtgggctcgg ctttcctgat gtgtgtcgac ctgttggacg
    12481 cagtgtcggg gcagggcccc tggccggcca cctgcagttg agggggatcg acactgttcg
    12541 cggccggcct cgtgatttcc tgcacgggct cagcggtccg tcgctgctca tgagtgcgag
    12601 tggggacgcg gtttggtgat gcggccggat tgcgtgcgcg acggtcggat ccgacgtgcc
    12661 cgccctgcct cggttttccc tgtcacgcct cggcggcgtc tcgcagtggc agcagggcgg
    12721 agagcgacag ttcgtgcagg cgtcggtgag ccgcgtcatc ccacgcgatg ggcaactggt
    12781 tctttgcctg caggtagtgg agggtgaaca cctccaggta ggcctccagc gtctgctcgg
    12841 caagagattc gcctgccagc ttcaggcaaa gggcggccac ctcgctcgtg cagaagtggt
    12901 cgccgcggtg ggagcggcgc agcttgtact gcgacagctg gtccggattg aggctgagca
    12961 ccggcaggtg gtccaggtaa ggactcttgc ggaacatctt gcgtgcctcg gaccaggtgg
    13021 catcgagcag cacgaacagc gggcgcttgc cggatccgcc ctcgagggtg tcggccgttt
    13081 gaacgctgtg gacgacccgt tcgggcgcca cgtactcgcc cggaaacacc acgtacggct
    13141 gccattgcgg atcgctcagc aggctcagca gatcggggtc tgtttcggtt cgagcccagc
    13201 cgaaggcgaa ggtatcggcc acgacatcgg cgatcagcca gccggtgttg ctcggcttga
    13261 gcggctcgat gtcagccatc agcaggcaca cgcccgagcg ggtgggcacg gccggtcgca
    13321 aggcgcacat gcagtggctg ggcagcagtc ggcagcccgc acaacgctcg cgcttgaagc
    13381 cgccgcgggc gagaaagggc ttggcgctgc gcgccagtcg ggcggcgcgc aggcgggaga
    13441 cggcgtgagg catgcggagc tttcagggag aggcgcagcg acggcagatc ccgccgctgc
    13501 gatgcatctg cgcgtatttc acgagatgcc cggcgaccca gttggactcg gcgggaaacc
    13561 tgcgagccat cagatcgcga aggggatggt attccgggtc gaaggctcac aatcgcaaag
    13621 tcatcgacac cgctgtcgac ggccattcct gatacgcgat gagccagccg atgagtgccc
    13681 tgcccaactg tccccaatgc aattcggaac tgacctacga ggacggatcg cagttcgtgt
    13741 gcccggagtg cggccacgaa tggtccgggg aagccgcgac tgaagtggca gaggctctgc
    13801 gcgtggtgcg agacgcccat ggcacggagc tgaaggatgg cgacaccgtc accgtcatca
    13861 aggacctgaa gatcaaggga tcttccctgg tcgtgaaggt cggcaccagg gtcaagaaca
    13921 tccgtctggc cgagggtgac cacgacatcg attgcaagat cgatggcatc ggggcgatgg
    13981 gtctgaagtc tgaattcgtg aagaaggcct gagcgcctcc cttgtcatcg catgggccaa
    14041 ccttggcctg cggctgcggt caggcggcat ggagacagcc ccaagcgttc cgcggcctgc
    14101 tgatgtggtg aggcggatcg cctttgccac gtgctccgga attgatccag gtcaaacctg
    14161 caagcgctga catcgaaagg acacggatcc gtcaattcac tttcactggc gaaggtcact
    14221 ctgccgtcat caacaacgcc aaccggagag cgaacgatgg gtgaattctg gaagaaggtc
    14281 gagatcctgc gctgggacga cagccgctat taccaccagt gccgcatcaa ccagaccttg
    14341 catctggcga gcgcgatcag cttcgtcgcc gcctacgtgc tgatgttcgt ggacccagcc
    14401 tcggccgcac tggtgggctg gctggtcggc atgatcacgc ggcaatcggg acacatattc
    14461 ttcgagccgc gcggcttcga ctcgatccac ggcgtgagct accagcagaa ggagtcgatc
    14521 aaggtcggct acaacatgcg ccgcaaatcc gtgctgatcg gcgtgtggtt gtgcctgccc
    14581 gcgctgctcc tcgccagccc cagcctgttc ggcctgatcg aatccgctgc tggctggcag
    14641 ggctacgccc gcgatgtcgg gctgagctgg ctggcgctgg gtatcggcgg actgctgttc
    14701 cgcaccgtgc acctgttctt cctgcgcggc ccggcctggg gcctggcctg ggctgtgaag
    14761 atcctgaccg acccgttcca caacatcgcg atctactgga agtcacccct ggcgctgctg
    14821 cagggccagc ggttcgaccc gatggggacg caccgccatc ctgcggagac ttgaacgcgc
    14881 gctgggcgac agcggcagaa ggagcggcgc cttcggacgc cagcgtcagg ctcgccgctg
    14941 caccctgccc gatgacccgc cgctcttcga ggtgaacacc gaactgcgcc tctcgaagtc
    15001 attcatgtcg gcttcggcgc gggctgggcg ttcttcgtcg aatgcgtttg cttcctggcc
    15061 acgaagggca cggccacccc gccagaaagc gcttgcggcg cgcctgctgt gtccatgccg
    15121 cccatgacag gcatcctgtc atcaggaccc gttcgtgtgc ctctgtgcga gccatcgcac
    15181 aggttatccg gccgttgttg tcggccgcat caacggccag gcccagcgtg ctctgcacac
    15241 cggctgcgtc gtcaatcagc tgccgcaggc gtgtctgaag attcgcatga ctgggcgtgg
    15301 ccgattgcag tccgtattca gcagcggctc gcgacccggg cggcgacacc atgtgccgag
    15361 caaggtgcac acatgcctcc ccttcagcac ctgctcattg acccggggat tgctcttttc
    15421 cgaaggtccg aatgttgctg acctcgtcgg cagggagcac gggttgattc aaatggattg
    15481 gcgaggctgg cctggcctgc tctgcggcat catgcaggca tgaacaagac caccggcggt
    15541 gagctcgccg ccgtactcaa ggcagaaact ggtgcgctgc attccctgac cgaacgcagt
    15601 ggagcaatgg cggacttgct gcgcgggcgg ctcagtctgc atgactactg cgtcatgttg
    15661 cgcaatttgc agcccatcta tgcggtgttg gaggcgggcc ttgacgagca tgcagaagat
    15721 gccaggcttt ggcgcccgga attgcgtcgg cttcatgccc tggagcagga ccttgaccag
    15781 ttgcatgccg gcgactggcg atccgaattg atagtggcgc cgactgccgg gctgtacgtc
    15841 acgcgattga cagcgctgat tcacaaggag cccttgctgc tgctggcgca tgcctacgta
    15901 cgctacctgg gcgacttgag cggcggccag atgctctcgg ggctgattcg ccgacagtat
    15961 gggcttgatt gccaagctgg gactgccttt tacgcctttg gcgagccctc gcatgtcgac
    16021 gcgctgaagc gcgattttcg cgatggattg gatgcgctga ccctgatgcc aacgcaacgc
    16081 gcggctttcg tggaagaggc ccgtgaggca tttcgcctgc accagcgact cttcgaggaa
    16141 ctgcgcgcat agccatctgc gctgcgcagc gtgtcgatca acgggacgcc tgcgcgcagg
    16201 tctatgagaa gaagcttcgg atctgcacgt aaagccccgt agcgcgccgc gtcgagtctt
    16261 ccagctcctg cagaagcggc gcgactggta cgccgccccg tgcgtccgca atgtccatcg
    16321 ctgccaggct tgcattggtg aggatggccg agatgacctc atcagcctcg gcaggtgagt
    16381 tcactgcagc cagtcgcaaa gctgtttcca gattgagccg agcggtgtca gcacggcgtg
    16441 cctcacgaag atcgaccagt ctcaacacaa agccgagcag cttgccgtcg cggccactca
    16501 ccggttcggc atgcacgtcc atgggcaccg cgctgccgct gcctgtcgac aagtcgagtt
    16561 ctcgtcgcca gccatgtttg gaggttcgca tctgctcaag tgcctgcgat attgctggtg
    16621 aattcgtgaa cagtgcttcg ataggaactt ctctgtccac ggctgaccca cgcacagcca
    16681 ccagggcgtc gtttgcgaac actgcacggc cttgcgcgtt ggccaccagc accggctcgc
    16741 gcgagcccgc tacagtgcca cggatctgca ccagttgatg ctctgcgatc aacaggcgta
    16801 ccgcatgcac ttgggcgatg atgtccacca aggcaatgcc aatgccgcgc gcgagcgcca
    16861 gttctgtagg cgtccagggc acagccgtgc ctcgcacgat ttccgaccag gccgcgaatg
    16921 agcggcgcgg ctcaagctcc agcgggttgt tggcaaccat ggacttggat ggttcgccag
    16981 cccaggtgac gctttgcagc tgttccttgc gaaaccaaat caaccagtcc gggctgttgt
    17041 ttgacagcgg cacagccagt acgccgctcg ccgttggcgt caggctggac agtgcgggat
    17101 tcttctgggc gatcgatgag caggaaaagg gcgcatcgct gggctgcggt tgatcctgca
    17161 cccaggcaag cagcgcacga agttcgggcg tggacggtgt ttctcccgct gtcagtacct
    17221 caccttcgca gaacagtgcc gcccccgtcg cttccagagg ctgcaacaag gtgcgcgggt
    17281 tacggaacag ggcgtagcgc cagtcgccct ctatcgaggt tgcctcgatg aggctgctcc
    17341 aaccggcgca ccatcagcgc cacctgcgca tgggcatagt tttcga
//
LOCUS       DAOORV010000078        17335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4002_length_17335_cov_10.836574, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000078 DAOORV010000000
VERSION     DAOORV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_17335_cov_10.836574"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /gene="ssb"
                     /locus_tag="PKV56_11355"
     CDS             complement(<1..202)
                     /gene="ssb"
                     /locus_tag="PKV56_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOX68456.1"
                     /translation="MASINKVILIGNLGRDPEVRYTPNGNAICNISVATTRNWKDKTS
                     GDKVEETEWHRVVFYDRLAEIAG"
     gene            422..1609
                     /locus_tag="PKV56_11360"
     CDS             422..1609
                     /locus_tag="PKV56_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOX68457.1"
                     /translation="MASRCSASALGPWLTLLWTPAVAVAVLWWTRRFVPGAAGSGIPA
                     VIAALDDRLPEERRPRFVSLRLAMHKIGLVSGSLLAGLSVGREGPTVQVGAGVMLHAQ
                     RWLSPRAGIDAHDLMVAGAAAGIAAAFNTPLGGLIFALEQLSRRRQATHSATVLISIV
                     LAGLVAVSVFGNVSHFGQLRVQHLGLELLGPGLLVALSAGIAGGLLSRLVVASARGLP
                     DRFSRWRAQHPLRFGAGCALAVAVIGITSNGATSGAGYEPTRALLEGSSEAPGVFTLL
                     KFCATWLSVWSGVPGGIFAPSLAIGASLGHDIALVTGVGAEAAIPLIALGMVGFLAAA
                     TQGPITAFIIVMEMVAGHAMVLSLMASALLASGVARWIAGSLYVDLAGLLMLPTEPPD
                     TRR"
     gene            1664..3613
                     /gene="recQ"
                     /locus_tag="PKV56_11365"
     CDS             1664..3613
                     /gene="recQ"
                     /locus_tag="PKV56_11365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOX68458.1"
                     /translation="MTSEFDVIDARLHEGSTDPHDAPTAHGRAALSVLQTVFGYTTFR
                     GAQAQIVDHVCAGGDALVLMPTGGGKSLCYQVPAIVRHRAGQGVAIVVSPLIALMHDQ
                     VGALEEAGVHAAYLNSSLTSEDAQRIEREMMGGRLVLLYAAPERITTPRFLAQLDSLH
                     ERGLLSLFAIDEAHCVSQWGHDFREDYLSLNVLHERYPGVPRIALTATADDLTRADII
                     ERLQLEEARVFISSFDRPNIRYGIVEKDNARAQLLRFIQDEHDGDAGIVYCQSRKKVD
                     ETAAWLSSEGITALPYHAGLDADVRRRHQDRFLREEGLVMVATIAFGMGIDKPDVRFV
                     AHLDLPKNIESYYQETGRAGRDGLPADAWMAYGLADVVNQRRMIDDSPAGDEFKRGQV
                     GKLEALLALAETHDCRRVRLLAYFGEPSQPCTRLQNACDNCLSPPVTWNATEAARKAL
                     SCIYRFHQNGGQRFGAGHLIDVLRGKSTDKVTQHGHASLSTFGIGSDVSEGQWRAVLR
                     QLIALGYLRTEGEYHTLELTGSARDVLRGEVQLLLREASAASAGGSGRRGRGSSRGGA
                     SKDKAPPVPLDADATERFTALKAWRAEVAREHNLPAYVVFHDATLAEMARACPQTLDD
                     IGEISGVGAKKLDAYGREILRVLAG"
     gene            complement(3650..4558)
                     /locus_tag="PKV56_11370"
     CDS             complement(3650..4558)
                     /locus_tag="PKV56_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOX68459.1"
                     /translation="MLIACGGATTVARAETASPTTEGTATVVEFDRGLLWRIDKATAP
                     GRGPNPSHLYGTVHIDDPRATAFSANVLRALEQSRVFLPELLPDAASAQAFIAATHAA
                     PGQTLETLAAPDDFERIAGRLGTRYGVPRSVASRLKPWAAYVYLSQPARPMGEIVDAA
                     LIRLAQQQGLAVRPLETVAQQIAAMEAVPVPSQLALVAALARDHDAALAEVDQLVELY
                     LAQDLAGMRRLEESAVRDDPTLRQPMADFMEQVLERRNERMLVTLLPQLEAGGAFAAM
                     GALHLTGEQGLLARLARAGWRVQRVD"
     gene            4887..5888
                     /locus_tag="PKV56_11375"
     CDS             4887..5888
                     /locus_tag="PKV56_11375"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HOX68460.1"
                     /translation="MSLGAVAQTTPARAPQSADANSWTFGLDNDWLALSHRDADYSGG
                     FLFTMSGPASAQFLLSLDPVLGFIDGLWMPGSAPTSKAALQFGAQAYTPKDKKSRDVV
                     RDDRPYAGLFSLTSTRMTVAGEADPVWSSAFTLGLLGTGLGKNLHRGFHRLIGSDTPN
                     GYDHQISDGGEPTLKYTVRRQALALDSRDLLGQRADLKWGVEGSLGYITEGALLVSGR
                     FGRFSTPWWSLYPERSEYYAPPSSKYGGDLYLMYGVGARVRGYNSLIQGQFRDSDHTL
                     GGSKLETFIGEAYAGVTWGITSSTSLSYLVRYQSSEIKHGTGSRDIYNGSLYLNIGY"
     gene            5968..8937
                     /gene="uvrA"
                     /locus_tag="PKV56_11380"
     CDS             5968..8937
                     /gene="uvrA"
                     /locus_tag="PKV56_11380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOX68461.1"
                     /translation="MPTPPPSPTQVTQPQAAGRSSSLEAHSIRVRGARTHNLKNIDLD
                     IPRNRLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDIIEG
                     LSPAISIEQKATSHNPRSTVGTVTEIHDYLRLLYARAGTPFCPDHRMALQAQSVSQMV
                     DQALALPEGTRLMVLAPVVRDRKGEFAELFADMQAQGYVRFRVDGTAYESADLPRLKK
                     NEKHDIDVVLDRVKVSAAADGGLQQRLAESFEAALRIADGRAMAFEMDHDREHLFSSK
                     FACPVCGYSIAELEPRLFSFNSPVGACPACDGLGHVTVFDPQRVVGFPGLSLASGAIK
                     GWDRRNSYTFSLLESVASHYRFDLDAPFETLPEVIRQVLLHGSGEEEIAFVYAADSVG
                     RDGSTKSRSVKRKHPFEGIIPTLERRYRETDSALVREDLARYQNARPCPECHGSRLRR
                     EARHVFLQEAGSDSAEPGEPIYRVEHSTLRDCQTYFDRLQLKGAKAEIADKVIREIRS
                     RLKFLNDVGLNYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRD
                     NDRLIGTLRHLRDIGNSVLVVEHDEDAIRAADHVIDMGPGAGAHGGRVMAQGTPAEVA
                     ANPESLTGRYLARALRIAVPARRHRLADSADPQTLRIANARGNNLRHVSVEIPVGLFT
                     CVTGVSGSGKSTLVNDTLYAAVARKLYNSHTEAEPHDSIEGLDAFDKVINVDQSPIGR
                     TPRSNPATYTGLFTPIRELFAEVPMARERGYGAGRFSFNVGAAAGGGRCEACQGDGVL
                     KVEMHFLPDVYVPCDVCHGRRYNRETLEVLYKGKNITEVLNLTVEDAHGYFNAVPNIA
                     RKLQTLLDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHF
                     ADIDLLLKVLHQLRDAGNTIVVIEHNLDVIKTADWIIDMGPEGGAGGGQVVAAGTPEA
                     VAANPASYTGRYLKPML"
     gene            complement(9078..9878)
                     /locus_tag="PKV56_11385"
     CDS             complement(9078..9878)
                     /locus_tag="PKV56_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOX68462.1"
                     /translation="MSAIPPSRRHYFVDEAGDPSLFDARGHVIVAQEGCSRHFMLGVL
                     DVADPVALAADLLALRQSLLSDPYFRDVPSMQPERRKTALAFHAKDDLPEVRREVFSV
                     LLRHELRFYAVVRDKQSVLAYVRQRNQVDPAYRYQPNELYDSLVARLFKDRLHLSPEV
                     DVCFAERGRSDRSAALMQALDRARTRFEVKWGRNVSSAISARQSSPMREASLQAVDYF
                     LWALQRRYERAEGRFLQLIWQKVGLVHAVDEIERAAYGTYYTKKKPLV"
     gene            complement(9875..12952)
                     /locus_tag="PKV56_11390"
     CDS             complement(9875..12952)
                     /locus_tag="PKV56_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX68463.1"
                     /translation="MSQVLINQYLAQLDVLKKVGGTHRESVVREAFKDLLKGWGKQHD
                     LVFIPEYKLDSATKDTRYVDGALLHELRVPFGYWEAKDAKDDLDAEIEFKFKRGYPQD
                     NIIFEDSTRAVLIQHRAEVMRCDVADVQALEKLLKLFFSFERAEIADFRKAVAQFKTD
                     LPAVLEALRSMIEHEHGSNAAFTQASQKFLKHAQDAINPSLTEADVREMLIQHILTEE
                     IFSKVFDSEFHRDNNVARELYKLEATFFTGGLKKSTLKGLEAYYAAIRAAAAQIGNHH
                     EKQTFLKVIYENFYKVYNAKAADRLGVVYTPNEIVRFMIEGADWLCEKHFQRNLIDKD
                     VEILDPATGTGTFICELLEHFRGQPKKLTHKYKEELHANEVAILPYYVANLNIESTYA
                     AITGQYAEFPNLCFVDTLDNVGLHTAAKGTTAELFGSVSEENVARIKRQNSRKISVVI
                     GNPPYNANQANENDNNKNREYPEVDARIKQTYIAESTAQKTKLYDMYARFFRWASDRL
                     DANGVLAFVTNRSFVESRTFDGFRKVVAQEFAELYVVDLGGDVRANPKLSGTKHNVFG
                     IQTGVAISFLVKRKGSKGGRVFYARRPEMETAEEKLAFMGSAALSRMAMDELRPDAMQ
                     NWLNLTSNDFDSLMPIASKDVKETTKASQEKATFKVFSLGVNTARDDWVYDLDQTTLK
                     AKADFLATQYSAQGRHDATFDASIKWSEALKLRHAASRTASSDTSCIRDAMYRPFTVA
                     NLLDSDLFIDRRGLAEQLFPRPPQTLGNLAICMIGVSTPKPFAVLATSRLPDLHLIGA
                     GAGASYFSLHRYESGIRIDNITDWSLAEFKKHYQPGRGKKAQPITKEAIFHYVYAVLH
                     DPQYRETYAQNLKREFPRIPLYGDSDSTFWQWAVWGEALMALHIGYEQVTPFALERTD
                     VPDEKVRAAGQPPRVILKADKDTGRIALDSETTLTGVPKEAWAYVLGNRCAIDWVLDQ
                     YKEKKPKDPTIRTRFDTYRFTNHKEQVIELLKRVTTVSVETMRIVGAMKGAGR"
     gene            13270..13854
                     /locus_tag="PKV56_11395"
     CDS             13270..13854
                     /locus_tag="PKV56_11395"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOX68464.1"
                     /translation="MLLMIDNYDSFTFNLVQYFAELGEDVRVFRNDELTLEGISELRP
                     DRLVLSPGPCSPNEAGICVPAIRHFTGKLPILGVCLGHQSIGAALGGKVVRAHVQMHG
                     KTSVISTDERGVYTGLPQKFTVIRYHSLAIELATLPEILEITATSEDGEIMGVRHREL
                     AGTATPLEGVQFHPESILTEHGHAMLKNFLDLRA"
     gene            13900..14934
                     /gene="trpD"
                     /locus_tag="PKV56_11400"
     CDS             13900..14934
                     /gene="trpD"
                     /locus_tag="PKV56_11400"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOX68465.1"
                     /translation="MPITDTEALARLVDHREIFHDEMLTLMRRIMSGEMPPATMAALL
                     IGLRVKKETIGEITAAAQVMREFSTKVEVADRRHLVDIVGTGGDGSHTFNISTCSMFV
                     AAACGARVSKHGNRSVSSKSGSADVLEALGVQLALPPDAIARCIATTGVGFMFAPAHH
                     PAMKNVAPVRRELGVRTIFNILGPLTNPADAPNILMGVFHADLVGIQARALQRLGARH
                     ALVVYGRDGMDEVTLGASTLVCEIKGDEVCEYEIHPEDFGLAMASHRALRVETPDDSK
                     RMLLAVLDNHPGPARDIVMLNAGAALYAADVAPSMADGIALAREALRSGSARDKLHEF
                     VGATQALAAA"
     gene            14946..15737
                     /gene="trpC"
                     /locus_tag="PKV56_11405"
     CDS             14946..15737
                     /gene="trpC"
                     /locus_tag="PKV56_11405"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOX68466.1"
                     /translation="MSSDILKRIVEVKRDEIRAARARRDQASLRRDAESLGGVRDFAG
                     AMRDKIAVGQAAVIAEIKKASPSKGVLREHFVPAEIAVSYARGGAACLSVLTDVQFFQ
                     GAVLYLQEARAACALPVLRKDFMVDPYQVFEARAMGADCILLIAACLDDAEMMDLEAQ
                     AHELGMAVLVEVHDGFELDRALSLKTPLVGINNRNLRTFEVTLDTTLGLLKHVPADRL
                     LVTESGVLARADVDRMRAADVSAFLVGEAFMRAADPGQALADLFG"
     gene            15784..16527
                     /locus_tag="PKV56_11410"
     CDS             15784..16527
                     /locus_tag="PKV56_11410"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOX68467.1"
                     /translation="MNGAPRLAASIDALVDRLRGDWRPCVEAWRSSAGGRALIARIDA
                     LVASGVTVYPAQVFRALETTPLASTRVVILGQDPYHGPGQAEGLAFSVPLGERIPPSL
                     RNVFKELQRDLRVTVPTSGHLGRWADRGVLLLNTCLTVEASSPASHAKYGWQALTDMI
                     ISTAANDRKPKVFMLWGAHAQAKTHLIDAAQAGHLVLQCNHPSPLAASRPPVPFIGCG
                     HFSSAVRFLERCDVGASGAAGATDIFDLR"
     gene            16576..16661
                     /locus_tag="PKV56_11415"
     tRNA            16576..16661
                     /locus_tag="PKV56_11415"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16610..16612,aa:Tyr,seq:gta)
     gene            16714..16787
                     /locus_tag="PKV56_11420"
     tRNA            16714..16787
                     /locus_tag="PKV56_11420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16746..16748,aa:Gly,seq:tcc)
     gene            16859..16933
                     /locus_tag="PKV56_11425"
     tRNA            16859..16933
                     /locus_tag="PKV56_11425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16891..16893,aa:Thr,seq:ggt)
     gene            16972..>17335
                     /locus_tag="PKV56_11430"
     CDS             16972..>17335
                     /locus_tag="PKV56_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX68468.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILASKFGGEA
                     KAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHIL"
BASE COUNT         2851 a         5586 c         5781 g         3117 t
ORIGIN      
        1 cgccggcgat ctcggccagg cggtcgtaga agacgacgcg gtgccattcg gtttcctcga
       61 ccttgtcgcc gctggtcttg tccttccagt tgcgggtggt ggcgacgctg atgttgcaga
      121 tcgcattgcc gttcggggtg tagcgcactt cgggatcgcg acccaggttg ccgatgagga
      181 tgactttatt gatcgaggcc atggtgcgag ctccggtgac agggtttcgc gacgattatc
      241 gacgcagccg cgttggccgg gcctccgatt gagtgcagct cgggcactgc taaggtagca
      301 gccatgcacg atccgcagcc cgatgtctgg cacaagttgc gcgccgagtt cgcggccggc
      361 cggcagtggg tcgatcgggg cgtggtgctc ggctacgcgg tgctcaccgg ctgcatcgtc
      421 gttggcttca cgctgctcgg cgagcgcact cgggccctgg ctcaccctgc tgtggacacc
      481 ggccgtcgcg gtcgcggtgc tgtggtggac gcggcgtttc gtgcccggtg cggcgggttc
      541 gggcatcccg gcggtgatcg ccgcgctcga cgaccgattg cccgaggaac ggcgccctcg
      601 cttcgtctcg ctgcggctgg cgatgcacaa gatcggactg gtctcgggca gtctgctggc
      661 cgggttgtcg gtcggtcgcg aagggccgac ggtgcaggtc ggcgccggcg tgatgctcca
      721 tgcgcagcgc tggctgtcgc cgcgtgccgg catcgatgcc cacgacctga tggtggccgg
      781 tgcggcggcc ggcatcgcgg cggccttcaa cacgccgctc ggcgggttga tcttcgcact
      841 ggagcagctg tctcggcggc gccaggcgac gcacagcgcc accgtgctga tcagcatcgt
      901 gcttgccggg ctggtcgcgg tgtcggtgtt cggcaacgtc agtcacttcg ggcagttgcg
      961 ggtgcagcac ctgggcctgg agttgctcgg tccggggctg ctcgtagcgt tgtccgcggg
     1021 catcgcgggc gggctgctgt cgcggctggt cgtcgcgtcg gcgcgcggcc ttccggatcg
     1081 cttcagccgc tggcgggcgc agcatccgct gcgtttcggc gcgggctgcg cgctggcggt
     1141 ggcagtcatc ggcatcacgt cgaatggcgc cacctccggg gcggggtatg aaccgacgcg
     1201 ggcgttgctg gagggttcga gcgaggcacc gggcgttttc accctgctga agttctgcgc
     1261 cacctggctg tcggtgtggt cgggcgtgcc gggcggcatc ttcgcgccgt cgctggcgat
     1321 cggcgcgagc ctgggccacg acatcgcgct ggtcaccggg gtcggggcgg aggcggcgat
     1381 cccgttgatc gcgctgggca tggtcggctt cctggcggcg gcgacacagg ggccgatcac
     1441 cgccttcatc atcgtgatgg agatggtcgc cggccatgcg atggtgctca gcctgatggc
     1501 gagcgccctg ctggccagcg gcgtggcgcg ctggatcgcc ggatccctgt atgtcgatct
     1561 tgccggcttg ctcatgttgc cgacggagcc acccgacacg cggcgctgaa ccgggcggag
     1621 ccgagcgccc gcttaccatc ggcaccatgc ctgcgactgc cccatgacct ccgaatttga
     1681 cgtgatcgac gcgaggctgc acgagggctc gactgaccca cacgatgcgc ccaccgccca
     1741 tggtcgcgcc gcgctgtcgg ttctgcagac cgtattcggc tacaccacgt ttcgcggtgc
     1801 gcaggcgcag atcgtcgacc acgtgtgcgc cggcggcgac gcgctggtgc tgatgcccac
     1861 cggcggtggc aagagcctgt gctaccaggt gcctgccatc gtgcggcatc gcgccgggca
     1921 gggcgtcgcc atcgtcgtca gcccgctgat cgcactgatg cacgaccagg tcggcgcgct
     1981 ggaagaggcc ggcgtgcatg cggcctacct gaattcgagc ctgacgtcgg aagacgccca
     2041 gcgcatcgag cgcgagatga tgggcgggcg gctggtgttg ctgtatgcgg cgcccgagcg
     2101 catcaccacg ccgcgcttcc tcgcgcaact cgattcgctg cacgagcgcg ggctgctcag
     2161 cctgttcgcg atcgacgagg cgcactgcgt gagccagtgg ggccacgact tccgcgagga
     2221 ttacctgtcg cttaacgtgc tgcacgagcg ctaccccggc gtgccgcgca tcgcgctcac
     2281 cgcgacggcc gacgacctga cccgggcgga catcatcgag cgcctgcagc tcgaggaagc
     2341 gcgcgtcttc atcagcagct tcgatcggcc gaacatccgc tacggcatcg tcgagaagga
     2401 caacgcacgt gcgcaactgc tgcgcttcat ccaggacgag cacgacggcg acgcgggcat
     2461 cgtctactgc cagtcgcgca agaaggtgga cgagacggcc gcgtggctga gcagcgaagg
     2521 catcactgcc ctgccgtacc acgccgggct cgatgccgat gtgcggcgcc gccatcagga
     2581 ccgtttcctg cgcgaggagg ggctggtgat ggtggcgacc atcgcgttcg gcatgggcat
     2641 cgacaagccc gatgtgcgct tcgtcgctca cctcgacctg ccgaagaaca tcgagagcta
     2701 ctaccaggag accggccgcg ccggccgcga cggcctgcct gcggacgcct ggatggccta
     2761 cggcctggcc gatgtggtga accagcgccg gatgatcgac gacagcccgg ctggcgacga
     2821 gttcaagcga gggcaggtcg gcaagctcga ggcgctgctg gcgctggccg aaacccacga
     2881 ttgccgccgc gtgcgattgc tggcctattt cggcgagccc agccagccgt gcacacggct
     2941 gcagaacgcc tgcgacaact gcctgagccc gcccgtcacc tggaacgcga ccgaggcggc
     3001 gcgcaaggcg ctgagctgca tctaccgctt ccaccagaac ggcgggcagc gcttcggtgc
     3061 cgggcatctg atcgacgtgc tgcgcggcaa gtcgacggac aaggtgacgc agcacgggca
     3121 tgcgtcgctc tcgaccttcg gcatcggctc ggatgtatcc gaggggcagt ggcgcgcggt
     3181 gctgcgccag ctgatcgcgc tgggctacct tcgcaccgag ggcgagtacc acacgctcga
     3241 actgaccggc agcgcccgcg atgtgctgcg cggcgaggtg cagttgctgc tgcgggaggc
     3301 gagtgcagcc tctgcgggcg gatcgggtcg ccgcggcagg ggttcatcgc gcggcggggc
     3361 ttcgaaggac aaggcgccac cggttccgct ggatgccgat gccaccgagc gcttcactgc
     3421 gctgaaggcc tggcgcgccg aagtggcacg ggagcacaac ctgccggcct atgtcgtgtt
     3481 tcacgatgcg acgctcgccg agatggcgcg tgcctgcccg cagacgctgg acgacattgg
     3541 cgagatcagc ggtgtcggtg cgaagaagct cgacgcctat ggacgcgaga tcctgcgggt
     3601 tctggcgggc tgaatccgcc cgccgagccc gtgccacgca cgcagcggtt caatccactc
     3661 gctgaacccg ccagcccgcc cgcgccagtc gggcgagcag gccctgctcc ccagtgaggt
     3721 gcaacgcacc catcgccgcg aaggctccac cggcttcgag ctgcggcagc agcgtgacca
     3781 gcatgcgttc gttgcggcgc tcgaggacct gttccatgaa atccgccatc ggctggcgca
     3841 gggtcgggtc gtcgcgcacg gccgactctt cgagtcggcg cattccggcg aggtcttgcg
     3901 cgaggtaaag ctcgacgagc tggtcgactt cggccagggc tgcgtcgtga tcgcgcgcca
     3961 gtgcggccac cagcgccagt tgcgacggga ctgggaccgc ctccatggca gcgatctgct
     4021 gcgccaccgt ttccagtggc cgcaccgcca ggccctgctg ctgcgccagg cggatcagtg
     4081 cggcgtcgac gatctcgccc atcggcctcg ccggttggct cagatagacg taggcagccc
     4141 agggcttcag ccttgaggcc acggagcggg gcacgccgta gcgcgttccc aggcgcccgg
     4201 cgatgcgctc gaagtcgtcc ggagctgcca atgtctcgag cgtctgcccc ggggcggcgt
     4261 gcgtggccgc gatgaatgcc tgcgcgctgg ccgcatcggg cagcagttcg gggaggaaga
     4321 cgcgcgactg ttccagcgcg cgaagcacgt tcgcgctgaa cgcggtggcc cgtggatcgt
     4381 caatgtggac cgtgccatac aggtgactgg ggttcgggcc ccgacccggg gccgtcgcct
     4441 tgtcgatgcg ccagagcagg ccgcgatcga attcgacgac cgtggctgtg ccttcggtcg
     4501 tcgggctcgc ggtctccgcc cgcgcgactg tggtcgcgcc gccacaggcg atcagcaagg
     4561 ccagcgcaac gagacggtac cccgccgacc gacccggagt gctccgcccg acaagcgaaa
     4621 tcaaccgcgt gatcggggca aagccaggcc atctgagcat gtgcagagtg tgcccggccg
     4681 ggccacggcc cgggatgtcc attgatgtca tcccggcttg cgctggcctg ggggtgttcc
     4741 tatcgtgtcc cgttctggta acggaatgtg cgcccctgcg gcatcatcga gcaatgaaaa
     4801 caagaaacac ccgcacgacc gctgccgttt tgcagcgcac ctcggcatcg atccgatgcg
     4861 catgcgcgct cctggctggt ctgtgcgtga gccttggcgc tgtcgcgcag accacgccag
     4921 cgcgcgctcc gcagtccgct gacgccaatt cttggacttt cggcctggac aacgactggc
     4981 tcgcgctcag tcaccgcgat gccgactact ccggcggctt cctgttcacg atgtccgggc
     5041 cggcgtcggc gcagttcctg ttgtcgctgg atccggtcct ggggttcatc gacggcctgt
     5101 ggatgcctgg atcggcgcca acgtcgaagg cggcactgca gttcggcgcg caggcgtaca
     5161 cgccgaagga caagaagtcc cgcgacgtcg tgcgcgacga tcgtccctac gccggtctgt
     5221 tctcgctgac gagcacccgc atgaccgtcg ccggcgaagc cgacccggtg tggtcctccg
     5281 cgttcaccct cggattgctg ggcaccgggc tcggaaagaa cctgcaccga ggctttcacc
     5341 ggctgatcgg cagcgatacg cccaacggct acgaccacca gatctccgac ggcggcgagc
     5401 cgacgctcaa gtacacggtg cggcgccagg cgctggcgct cgacagtcgt gacctgctcg
     5461 gacagcgcgc cgatctcaag tggggtgtcg aaggcagcct gggttacatc accgagggcg
     5521 ctctgctggt ttccggccga ttcggcaggt tctcgacccc atggtggtcg ctttatcccg
     5581 aacgctcgga gtactacgcg ccgccgagct cgaagtacgg gggggacctg tacctgatgt
     5641 acggcgtcgg ggcccgcgtg cgtggctaca actcgctgat ccaggggcag ttccgcgact
     5701 ccgatcacac gctgggcgga agcaagctcg agaccttcat cggcgaggcc tatgccgggg
     5761 tgacctgggg catcaccagc tcgacgagcc tgagctatct ggtccgctac cagtcctcgg
     5821 agatcaagca cggcaccgga tcccgagaca tctacaacgg cagcctgtac ctcaacatcg
     5881 ggtactgaag gctgacgcag cgagggggtg gccccttctg cgcgggaggt cggcccgtat
     5941 gatggctggc tacccgtttt gcagccgatg cccaccccac cgccgagccc gacccaggtc
     6001 acccagcccc aggccgcggg ccgttcctcg tcgctggagg cgcactcgat ccgggtgcgt
     6061 ggtgctcgca cccacaacct caagaacatc gacctcgaca ttcctcgcaa ccgcctggtg
     6121 gtcatcaccg gcttgagcgg ctcgggcaaa tcgagcctgg cgttcgatac gctgtatgcc
     6181 gagggacaga ggcggtatgt cgagagtctg agcgcctatg cgcggcagtt cctgcagctg
     6241 atggacaagc ccgatgtcga catcatcgaa gggctgagcc ccgcgatcag catcgagcag
     6301 aaggcgactt cgcacaaccc tcgctcgacg gtcggcaccg tgaccgagat ccacgattac
     6361 ctgcgcctgc tgtatgcccg tgccggcacg ccgttctgtc ccgatcaccg catggcgctg
     6421 caggcgcaga gcgtgagcca gatggtcgat caggcgctgg ccctgccgga gggcacgcga
     6481 ctcatggtgc tggcgccggt ggtgcgtgac cgcaagggtg aattcgcgga actgttcgcc
     6541 gacatgcagg cgcagggcta tgtgaggttt cgcgtcgatg gcactgccta tgaatcggcc
     6601 gacctgccca ggctgaagaa gaacgagaag cacgacatcg atgtggttct cgatcgggtc
     6661 aaggtgagcg ccgctgcgga cggcggcctg cagcaacggt tggccgaaag cttcgaggcg
     6721 gcgctgcgca tcgccgatgg gcgtgcgatg gccttcgaga tggatcacga tcgcgagcac
     6781 ctgttctcga gcaagttcgc ctgcccggtc tgtggctact ccatcgccga gctggagccg
     6841 cgactctttt cgttcaactc gccggtgggc gcgtgccccg cctgcgacgg cctggggcat
     6901 gtcacggtct tcgatccgca gcgcgtggtc ggattccccg gcctcagcct ggccagcggc
     6961 gcgatcaagg gatgggaccg tcgcaacagt tacaccttct cgctgctcga atccgtggcc
     7021 agccactacc ggttcgacct cgacgcaccc ttcgagacgc tgcccgaagt gatccggcaa
     7081 gtgctgctgc atggatccgg cgaggaagag atcgcgttcg tctacgccgc tgacagtgtg
     7141 ggtcgcgacg gcagcaccaa gtcgcgcagc gtgaagcgca agcatccttt cgagggcatc
     7201 atcccgacgc tagagcgccg ttatcgcgag accgattcgg cgctggttcg cgaagatctg
     7261 gcgcgctacc agaacgccag accctgcccc gaatgccacg gcagccggtt gcggcgcgag
     7321 gcacgccatg tgttcctgca ggaagccggc agcgactcgg ccgaacccgg cgaacccatc
     7381 taccgtgtcg agcactccac gctgcgcgac tgccagacct atttcgatcg gttgcagctg
     7441 aaaggggcca aggccgagat cgccgacaag gtgatccgcg agatccggtc gcggctcaag
     7501 tttctcaacg acgtgggact gaactacctg agtctcgacc gcagcgccga cacgctgagc
     7561 gggggcgagg cgcagcgcat ccggctggca tcccagatcg gctcgggtct gaccggcgtg
     7621 atgtacgtgc tcgatgagcc gagcatcggc ttgcaccagc gtgacaacga tcgcctgatc
     7681 ggcaccctgc gccacctgcg cgacatcggc aacagcgtgc tggtggtcga gcacgacgag
     7741 gacgcgatcc gggccgccga ccacgtgatc gacatgggcc cgggcgccgg cgcgcacggc
     7801 ggccgtgtga tggcgcaggg cacgcccgcc gaggtggcag ccaaccccga gtcgttgacc
     7861 ggccgctacc tggcgcgcgc gctgcgcatc gcggtgcctg cacggcggca ccggctggcg
     7921 gacagcgcgg acccccagac gctgcgcatt gccaatgcgc gcggcaacaa cctcaggcac
     7981 gtcagcgtcg aaatccctgt gggactgttc acttgcgtga ccggcgtgtc gggctcgggc
     8041 aagagcacgc tggtcaacga cacgctgtac gccgcggtgg cgcgcaagct ctacaactcg
     8101 cacaccgagg ccgagccgca cgactcgatc gaggggctgg atgccttcga caaggtcatc
     8161 aacgtcgacc agagcccgat cggccgcacg ccgcgcagca acccggccac ctacaccggg
     8221 ctgttcaccc ccatccgcga gctgtttgcc gaggtgccga tggcgcgcga gcgcggctac
     8281 ggcgccggcc gcttcagctt caacgtgggc gcggcggccg gcggcggccg ctgcgaggcc
     8341 tgccagggcg acggcgtgct gaaggtggag atgcacttcc tgcccgacgt ctacgtgccc
     8401 tgcgacgtct gccacggccg gcgctacaac cgcgagacgc tggaggtgct ctacaagggc
     8461 aagaacatca ccgaggtgct caacctcacc gtggaagacg cgcacggcta cttcaacgcc
     8521 gtgccgaaca tcgcgcgcaa gctgcagacg ctgctcgacg tgggcctggg ctacatccgc
     8581 ctcggccaga gcgcgaccac gctgtctggc ggcgaggcgc agcgcgtcaa gctggcactg
     8641 gagctctcca agcgcgacac cggccgcacg ctgtacatcc tcgacgagcc gaccaccggg
     8701 ctgcacttcg ccgacatcga cctgctgctc aaggtgctgc accagctgcg cgacgccggc
     8761 aacaccatcg tcgtgatcga gcacaacctc gacgtcatca agaccgccga ctggatcatc
     8821 gacatggggc ccgaaggcgg cgccggcggt gggcaggtgg tggcggctgg cacgccggag
     8881 gcggtagcgg caaaccccgc gagctacacg gggcgttatc tgaagccgat gctgtgaaca
     8941 accctgttga taagcggtgc acgcagggct aaacttcagc cgtccgggaa caaaattcgg
     9001 cttcatgccg tgtgtgctca ggctgagcgc cagacaccta tattcccggc acctcattca
     9061 ccgaaaaagc cgctgcgtca caccagcggc tttttctttg tgtagtaggt gccgtaagcc
     9121 gcccgttcga tttcgtcgac agcgtggacc agaccgacct tttgccagat caactgcaga
     9181 aagcgacctt cggcccgttc gtaacgacgt tgcaaggccc acaggaaata gtcgaccgcc
     9241 tgcaaagagg cttcccgcat gggagaggat tgccgtgccg aaatggccga actgacattc
     9301 ctcccccatt tcacttcgaa tcgcgtgcgc gcccgatcaa gggcttgcat caacgccgcg
     9361 gaccgatctg aacggccgcg ttcggcaaag cagacatcga cttcaggact caggtgcaac
     9421 ctgtccttga acagtcgcgc gaccagtgag tcgtagagct cattcggttg atagcgatac
     9481 gccggatcaa cctgattgcg ctgccgcaca taggccagca cactctgctt gtcgcgcact
     9541 actgcgtaga aacgaagttc gtgccgcagc aagacactga atacctcccg ccgcacttct
     9601 ggcaagtcgt ctttcgcgtg gaaagccagc gccgtcttgc gacgctcggg ctgcatggac
     9661 ggcacatcgc ggaagtacgg gtcgctgagc agcgattgac gcaacgccaa gaggtctgct
     9721 gccagagcca ctggatcagc gacgtcgagc accccgagca tgaagtggcg cgagcaacct
     9781 tcttgcgcaa cgatcacgtg accgcgtgcg tcgaacagcg aggggtctcc cgcctcatca
     9841 acaaaatagt gtctgcgcga gggcgggatc gcgctcaccg ccccgctccc ttcatcgccc
     9901 ccacgatccg catcgtctcc acgctcaccg tggtcacgcg cttcagcagc tcgatcacct
     9961 gttccttgtg gttggtgaac cggtaggtgt cgaacctcgt tcgaatggta gggtccttgg
    10021 gcttcttttc tttgtactgg tcgagcaccc agtcgatcgc gcagcggttg ccgagaacgt
    10081 aggcccaggc ctctttcggc acgccggtga gggtggtttc gctgtcaagg gcgatgcggc
    10141 cggtgtcctt gtcggctttc aggatgaccc tcggcggctg gccggcggcg cgcacctttt
    10201 cgtcgggcac gtcggtgcgc tccagtgcga agggcgtcac ctgctcgtag ccgatgtgca
    10261 gcgccatcag tgcctcgccc cagacggccc attgccagaa ggtgctgtcg ctgtcgccgt
    10321 acagcgggat gcgcgggaac tcgcgcttga ggttctgcgc gtaggtctcg cggtactgcg
    10381 ggtcgtgcag cacggcgtag acgtaatgga agatcgcttc cttcgtgatc ggctgcgcct
    10441 tcttgccgcg gccgggctgg tagtgcttct tgaactcggc cagggaccag tcggtgatgt
    10501 tgtcgattcg aatgccggac tcgtagcggt gcaacgaaaa gtagctcgct cctgcacctg
    10561 ctccgattaa atgcaggtcg ggcaacctac tcgttgctag taccgcaaat ggctttggcg
    10621 tcgacacccc gatcatgcag atcgcgaggt tgcccagcgt ctgcggcggc cgcgggaaga
    10681 gttgctcggc caagcctctg cgatcaataa agaggtccga atcgagcaga ttcgcaacgg
    10741 tgaacggccg atacatcgcg tcccgaatgc atgaggtatc cgatgaagca gtgcggctcg
    10801 cagcgtgacg aagtttgagt gcctccgacc atttgatcga cgcatcaaag gtcgcgtcgt
    10861 gtcgcccctg cgctgagtac tgcgttgcca agaagtcagc cttagctttg agcgtggtct
    10921 gatcgaggtc gtagacccag tcgtcgcggg ctgtgttgac gccaagcgaa aacaccttga
    10981 atgtcgcctt ctcctgcgag gccttggtgg tctcctttac atccttgctc gcaatcggca
    11041 tcaacgaatc gaagtcgttg ctcgtcaagt tcaaccagtt ctgcatcgca tccggccgca
    11101 actcgtccat cgccatgcga ctcaacgccg cactgcccat gaacgccagc ttctcctccg
    11161 ccgtctccat ctccggccgc cgcgcataga acacccgccc gcccttgctg cccttgcgct
    11221 tgaccaggaa gctgatcgcc acgcccgtct ggatgccgaa cacgttgtgc ttggtgccgc
    11281 tcagctttgg gttggcgcgc acgtcgccgc ccaggtcgac cacgtacagc tcggcgaact
    11341 cctgtgccac caccttgcga aagccatcga aggtgcggct ctcgacgaag ctgcggttgg
    11401 tcacgaacgc gagcacgccg ttcgcgtcga gccggtcgct cgcccagcga aagaagcgcg
    11461 cgtacatgtc gtacagcttg gtcttctggg cggtgctttc ggcgatgtag gtttgcttga
    11521 ttcgcgcgtc gacctcgggg tattcgcggt tcttgttgtt gtcgttctcg ttcgcctggt
    11581 tcgcgttgta cggcgggttg ccgatgacga cgctgatctt gcggctgttc tggcgcttga
    11641 tgcgggcgac gttttcctcg ctgacgctgc cgaacagctc ggccgtggtg cccttggcgg
    11701 cggtgtgcag gcccacgttg tcgagcgtgt ccacgaagca caggttggga aattcggcgt
    11761 actggccggt gatggccgcg taggtcgatt cgatgttcag gttggccacg tagtacggca
    11821 ggatggccac ctcgttggcg tgcagctcct ccttgtattt gtgcgtcagc ttcttcggct
    11881 gaccgcgaaa gtgctcgagc agttcgcaga tgaaggtgcc ggtaccggtg gccgggtcga
    11941 ggatctccac gtccttgtca atcaggttgc gttggaagtg cttctcgcac agccagtcgg
    12001 cgccttcgat catgaagcgc acgatctcgt tgggcgtgta gaccaccccc agccggtccg
    12061 ccgccttggc gttgtagacc ttgtagaagt tctcgtagat caccttgaga aacgtctgct
    12121 tctcgtggtg gttgccgatc tgcgccgccg ccgctcggat ggcggcgtaa taagcctcca
    12181 gccccttgag cgtgctcttc ttgaggccac cggtgaagaa ggtggcttcc agcttgtaga
    12241 gctcgcgcgc gacgttgttg tcgcggtgga actcgctgtc gaacaccttc gagaagattt
    12301 cttccgtcag gatgtgctgg atcagcatct ctcgcacgtc ggcttcggtc aggctgggat
    12361 tgatcgcgtc ctgcgcgtgc ttcaggaact tctgcgacgc ctgggtgaag gcggcgttgc
    12421 tgccgtgctc gtgttcgatc atcgagcgca gggcctccag cacggcgggc aggtcggtct
    12481 tgaactgggc gaccgccttg cggaagtcgg caatctcggc tcgttcgaag ctgaagaaca
    12541 gtttcagcag cttttccagc gcctgcacgt ccgccacgtc gcagcgcatc acctctgcgc
    12601 ggtgctggat cagcacggcg cgcgtgctgt cctcaaaaat gatgttgtct tgcgggtagc
    12661 cgcgcttgaa cttgaactcg atctcggcgt cgaggtcgtc tttcgcgtcc ttggcttccc
    12721 agtagccgaa gggcacgcgc agttcgtgta gcagcgcgcc atcgacatag cgggtgtctt
    12781 tggtggcgct gtcgagcttg tattcaggaa tgaagaccag atcgtgctgc ttgccccagc
    12841 ctttgagcag gtccttgaag gcctcgcgca ccaccgattc ccggtgcgtg ccgcccacct
    12901 ttttcagcac gtcgagctgg gcaaggtact ggttgatcag gacttgactc atgggcccgg
    12961 ggtgcaggga gtggtggcgt tggattgcaa cacaccctca ggcgctacgc gtaccgcggc
    13021 gcccggccgc ccgcgggctt cgtggccagt cgataatgca gcatgcgttc agtctcaaca
    13081 gagtcccaca ggcaccgttg gcgcaggcca tcgacctgac cgacttgttg cgcccacttg
    13141 ggcgctccgg actcttccgc cccgcgccgc acgctggcca tcgcagccgc gaaaatagcg
    13201 aatcacgagg cggacaccga aaccgggcga cccgttgacg cccatcgcaa tccacaggac
    13261 tgagcgcaca tgctgctgat gatcgacaac tacgacagct tcaccttcaa cctggtgcag
    13321 tactttgccg agttgggtga ggacgtccgt gtcttccgca acgacgagct gacgctcgag
    13381 ggcatcagcg aactgcggcc cgaccgtctg gtgctgtcgc ccggcccctg ttccccgaac
    13441 gaggcgggca tctgcgtgcc ggcgatccgg catttcaccg gcaagctgcc gatcctcggt
    13501 gtctgtctcg gccaccagag catcggtgcg gcgctgggtg gcaaggtcgt ccgcgcccat
    13561 gtccagatgc acggcaagac cagcgtgatt tccaccgacg agcgcggcgt ctacaccggg
    13621 ctgccgcaga agttcacggt catccgctac cactcgctgg cgatcgaact ggcgacgctg
    13681 cccgagatcc tggagatcac cgcgacatcg gaagacggcg agatcatggg cgtgcgtcac
    13741 cgggagctgg ccggcactgc gacgccgctc gaaggcgtgc aattccaccc cgaatccatc
    13801 ctgaccgagc acggtcacgc gatgctgaag aacttcctgg atctgagagc gtgacctcgc
    13861 gggcccgcct gtcatcctga gtacctttcg gagcctgtca tgcccatcac cgataccgag
    13921 gccctcgctc gcctcgtcga ccatcgcgag atctttcacg acgagatgct gacgctgatg
    13981 cgtcgcatca tgagcggcga gatgccgccg gcgacgatgg ccgcgctgct gatcggcctg
    14041 cgcgtcaaga aggagacgat cggcgagatc actgccgcgg ctcaggtgat gcgcgagttc
    14101 tcgacgaagg tggaggtcgc cgacaggcgt catctggtcg acatcgtcgg aaccggagga
    14161 gacggctcgc acaccttcaa catctcgacc tgcagcatgt tcgtcgcggc cgcctgcggt
    14221 gcgcgcgtca gcaagcacgg caaccgcagc gtgtcgagca agtcgggcag cgcggatgtg
    14281 ctggaggccc tcggcgtgca gttggcactg ccgcccgacg cgatagctcg atgcatcgcg
    14341 acgaccggcg tcggcttcat gttcgcgcca gcgcatcacc cggcgatgaa gaacgtcgct
    14401 ccggtgcgac gtgaacttgg cgtgcgcacg atcttcaaca tccttgggcc tctgaccaat
    14461 ccggcggacg cacccaacat attgatgggc gtgtttcatg ccgatctggt cggtatccag
    14521 gcccgcgcgc tgcagcggct gggcgcccgg catgcgttgg tggtgtatgg ccgcgacggt
    14581 atggacgagg tgacgctggg cgcgtcgacc ctggtgtgcg agatcaaggg cgacgaggtt
    14641 tgcgagtacg agatccaccc ggaagacttc gggctggcga tggccagcca ccgtgctctc
    14701 agggtcgaga ccccggatga ttcgaagcgc atgctgctgg ccgtgctcga caaccacccc
    14761 ggccctgcac gcgacatcgt gatgctgaac gcgggggccg ctctgtacgc cgcagatgtc
    14821 gcgccaagca tggccgatgg catcgcattg gctcgcgagg cgctgagatc gggcagcgcg
    14881 cgcgacaagc tccatgagtt cgtcggtgcc acgcaggcgc tggctgcggc ctgaggatcg
    14941 acgagatgag cagtgacatc ctgaagcgca tcgtcgaggt caagcgcgac gagattcgtg
    15001 cggcgcgcgc cagacgcgat caggcctcgc tgcggcgcga tgccgaatcg ctgggaggcg
    15061 tgcgcgattt cgcaggcgcg atgcgcgaca agatcgctgt tggtcaggcg gcggtcatcg
    15121 cggaaatcaa gaaggccagc ccgagcaagg gcgtgctgcg cgagcatttc gtgccggcgg
    15181 aaattgccgt gagctatgcg cgcggcgggg cggcctgcct cagcgtgctg accgacgtgc
    15241 agttctttca gggcgcggtc ctctatctgc aggaagcccg cgccgcctgt gcgctgccgg
    15301 tgctgcgcaa ggatttcatg gtcgatccgt accaggtctt cgaggcgcgc gcgatgggtg
    15361 ccgactgcat cctgctgatc gctgcctgcc tcgatgatgc ggaaatgatg gatctggaag
    15421 ctcaggcgca cgagttgggc atggccgtgc tggtcgaggt gcacgatggt ttcgaactgg
    15481 atcgcgcgct gtcgctgaag acgccgctgg tcggcatcaa caaccgcaac ctgcgcactt
    15541 tcgaggtgac gctcgacacg acgctcggct tgttgaagca cgtgcccgcc gaccggctgc
    15601 tggtgaccga gagtggcgtg ctggcacgcg ccgacgtcga ccgcatgcgc gctgccgacg
    15661 tgtcggcttt cctggtcggc gaggccttca tgcgcgcagc cgatccaggg caggcgctcg
    15721 ccgacttgtt cgggtgacgc gaatcgcggc atcggattgc agtcgggcat ttctggctca
    15781 ccgatgaatg gtgccccgcg tctcgccgcg tcgatcgacg cgctggtcga tcgattgaga
    15841 ggcgactggc gaccttgcgt cgaggcctgg cgcagcagcg ccggggggcg agcattgatc
    15901 gcgcgcatcg atgcactcgt ggcgagcggc gtgacggtct atccggctca ggtgttccgt
    15961 gcgctggaga cgacgccgct tgccagcacg cgcgtggtga tcctcggcca ggacccgtat
    16021 cacggtccgg ggcaggctga agggttggcc ttttctgttc cgctggggga gcgcattcca
    16081 ccgagtctgc gcaacgtgtt caaggagttg cagcgcgatc tgcgcgtgac ggtgccgacc
    16141 agcgggcatc tgggccggtg ggccgatcga ggagtcctgc tgctcaatac ctgcctgacg
    16201 gtcgaggcga gttcgccggc aagccacgcg aaatacgggt ggcaagcgct cactgacatg
    16261 atcatctcga ccgcggcgaa cgatcgcaag cccaaggtct tcatgctctg gggggctcat
    16321 gcccaagcca agacgcactt gatcgatgcg gcgcaggccg gccatctggt gctgcagtgc
    16381 aaccatccgt cgccgctggc ggcctcgcgc ccgccggtgc ctttcatcgg gtgcgggcat
    16441 ttctcgtcgg ccgttcgatt tctcgagcgc tgcgacgttg gtgcgtcagg ggctgccggt
    16501 gcgacggaca tcttcgatct gcgttgaggc ggtcgccagc agcaatttgc cgtgataaac
    16561 tcttgggctc gccgcggagg ggtggccgag tggttaatgg cagcagactg taaatctgcc
    16621 ctctaacgag tacgctggtt cgaatccagc ctcctccacc atggcgaaaa gaatagtttt
    16681 gaccctctcg ggccttggct cgaggaggat cgggcgggag tagttcaatg gtagaaccct
    16741 agccttccaa gctaatgacg cgggttcgat tcccgtctcc cgctccaggg ttcgggccgg
    16801 tttccatctg gatccgatga ggatcggggt gcgggctggc agacaagttg taggttaggc
    16861 ccatgtggct cagtggtaga gcactccctt ggtaagggag aggtcgcgcg ttcgatccgc
    16921 gccatgggca ccaagtcttg atattcattc tcgttttcga ggagcgatgt gatggcaaaa
    16981 ggcaaattcg agcggaccaa gccgcacgtg aacgtgggga cgattggtca cgtggaccac
    17041 ggcaagacga cgctgacggc ggcgatcacg acgatcctgg cgagcaagtt cggcggcgaa
    17101 gccaaggcgt acgaccagat cgacgcggcg cccgaagaga aggcgcgcgg catcaccatc
    17161 aacaccgcgc acgtcgagta cgagaccgcc aaccgccact atgcgcacgt cgactgcccc
    17221 gggcacgccg actacgtcaa gaacatgatc accggtgctg cgcagatgga cggcgccatt
    17281 ctggtgtgct cggccgctga cggcccgatg ccgcaaacgc gcgagcacat cctgc
//
LOCUS       DAOORV010000079        16784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4168_length_16784_cov_7.044832, whole genome shotgun sequence.
ACCESSION   DAOORV010000079 DAOORV010000000
VERSION     DAOORV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4168_length_16784_cov_7.044832"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..417
                     /locus_tag="PKV56_11435"
     CDS             52..417
                     /locus_tag="PKV56_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOX68469.1"
                     /translation="MPQNLRRFHVGDRLSEMAIFHNVVYLAGQVPSDTRQDMAGQTRQ
                     VLAMVDRLLAEAGTDNAHILMATIYLADMADFDAMNLVWDDWVAPGDAPPRATVQARL
                     ARPEWKIEIVVTAALSAGA"
     gene            420..773
                     /locus_tag="PKV56_11440"
     CDS             420..773
                     /locus_tag="PKV56_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOX68470.1"
                     /translation="MAAHRMIRVLLIAAAAYVLLVAMQGRTDKQAGAALAALAKPGDI
                     VMLSSETCMHCDQARRWLTEYGVPFRECFIERDAACADSYRAQQSPGTPTLLVRGERQ
                     VGFDPGLVTKAISGG"
     gene            complement(794..1426)
                     /locus_tag="PKV56_11445"
     CDS             complement(794..1426)
                     /locus_tag="PKV56_11445"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOX68471.1"
                     /translation="MSQHFELHPDNPQPRLLKQAAQILHDGGVAAVPTDSSYALVCRL
                     DDKSAVENLRRIRGVDDKHHLTLLCRDLSELASYARVDNRQYRLLKLGTPGPFTFILE
                     ASKEVPRRISHPSRRTIGLRVPEHKALQELLAVYGEALVATTLIPRDETEPLNDPDDI
                     RERYQKVLQAVVDAGACAAQPTTVVDLTGDTPVLVRVGRGDPAALGLHAD"
     gene            complement(1485..2357)
                     /locus_tag="PKV56_11450"
     CDS             complement(1485..2357)
                     /locus_tag="PKV56_11450"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HOX68472.1"
                     /translation="MKVPTAWINADLHCHSCVSDGTLTPEALAQRAKANGVELWALTD
                     HDEVGGQRRASDAAEAVGLPYLPGVEISVTFAGLTVHIVGLGIDPENAALIKGLADTR
                     DGRIARAHEMADGLAKVGIRDAFEGALNYAGNPELIARTHFARYLVETGVCASTHEVF
                     RKYLVEGKPGFVEHRWASLGNAVKWITQADGIAVIAHPGRYKFSVNAEYALFTEFKGH
                     GGRGVEVVTGSHTSADYDKYATLAIEFDLLASRGSDFHSPEESRTDLGTLAALPARLT
                     PVWEALAERVLLPA"
     gene            complement(2456..3670)
                     /locus_tag="PKV56_11455"
     CDS             complement(2456..3670)
                     /locus_tag="PKV56_11455"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOX68473.1"
                     /translation="MPHPATPGRCVNTLANPRRRELIEAGWKLSALATAGAGLLVGAS
                     ACGGGSASTPPAPVATAARAVVLTRALSSPWGLAFLPDGRMVVTQKGGSMVIVSADGA
                     TVSAALTGVPPVASAGQGGLLDVATDPDFTTDPWVYWSYAEAGLAGSGTAVARGRLSG
                     LALTDVQVIFRQLPKVVGNGHFGSRLVFRGDKTLFVTLGERQKGSPAQDLSTHLGKVV
                     RIARDGSVPAGNPDLGATARPEIWSLGHRNAQGAALHPTTGELWLVEHGPQGGDELNR
                     VAAGANHGWPVKSYGCNYGDPVGEACRIGGGTHAPTYAEPVSFWVPTSIAPSGLAFYT
                     GAGFPEWQGNAFLGALAGTALWRVVLNGTAEVSRERMFAGLGERIRCVRQGPDGWLYL
                     LTDSGKLIRVVR"
     gene            complement(3795..5564)
                     /gene="recJ"
                     /locus_tag="PKV56_11460"
     CDS             complement(3795..5564)
                     /gene="recJ"
                     /locus_tag="PKV56_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOX68474.1"
                     /translation="MNSPTLTLRPAAPRSAWALEQAGVHPLLARLFAARGVRGIEELD
                     DGLARLLPPAGLRGAAEAAVLLADALHERLRLCIVADYDCDGATACAVALRGLALLGA
                     APGTLGYVVPDRAVHGYGLTPAIVDLALAQGAQVLVTVDNGIASLAGVAHARAHGLAV
                     VVTDHHLPAQEDDAIVLPDADVIVNPNQPGCGFASKHLAGVGVMFYVLMALRSELRAR
                     GLFADASGGPSSAPAQPKLDPLLDLVALGTVADVVRLDANNRRLVAQGLRRIRTGRMQ
                     AGVAALFAAAGRDPSRAAGFDFGFALGPRINAAGRLSDMTLGIECLLTDDPARAAQLA
                     QQLDAINRERREVETGMREQAEALLDTLMQGMNDPGAAPPALAIYDPDFHEGVVGIVA
                     SRLKDRVHRPTFVFARGQDGLLKGSGRSIEGFHLRDALDLVSKRHPGVLKKFGGHAMA
                     AGCTLAEADFATFDAAFQQAALEGLDAATLERRLLSDGPLGIEHFTTDTVQALNAQVW
                     GPSFEAPLFSDEVEVVSQRLVGEKHLKLSVKHSGTLRDAIWFGHVDPVPARVTLAYRL
                     SIDEYNGRQRLQMIVEAAQATGA"
     gene            complement(5561..6511)
                     /locus_tag="PKV56_11465"
     CDS             complement(5561..6511)
                     /locus_tag="PKV56_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68475.1"
                     /translation="MHLLIPHASAVSEASAHTLRDLALPNLARLLTRLQPTTRDVDDE
                     YTLSPPHERALATAQGWHGADGRWPWAALQARADGIDVGRRPWGQVTPVHWHVGREHI
                     TLADPLALALPADESRTLFDAVRPLFDDDDGSLAWAAPDRWYLSHEQLADLPCASIDR
                     VIGRNVDLWLRGDPQASVEQLARVRWVRRLQNEVQMLLHQHPVNEAREARGALPVNSF
                     WLSGCGRAQSASAEVTVNDRLRAPLLAEDWAGWAESWRALDADALGPLVAAADRGEGV
                     SLTLCGERGWQRYDAMQRSLWQRLGDRFRTVDPHATLTAL"
     gene            7175..8431
                     /locus_tag="PKV56_11470"
     CDS             7175..8431
                     /locus_tag="PKV56_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOX68476.1"
                     /translation="MQWPYELQIGWRYTRAGRSGRRNGFISFISGVSMLGIALGVAAL
                     IIVLSVMNGFQKEVRDRMLSVISHVEVYQSKGEALPDWQATAAQARLSPQVVGAAPFI
                     AAQALIARGDDMRGAVVRGISPIEEATVTELAAKLRDTTLARLVPGGWGVVLGGELAR
                     GLGVRVGDKVTIVAPGGQVTPAGVVPRLKQLTLVGTFEAGHYEYDNGLALIHVEDAAR
                     LFRVEGPTGVQLRLKDAQQARAVADELQVRLGPSVRLSDWTRTNRNWFQAVQIEKRLM
                     FIILTLIVAVAAFNLVSTLVMTVTDKQADIAILRTLGASPRSIMAIFMVQGAASGVIG
                     TLAGMVLGLAVAFNIGVIVPAIERALHMSFLPSSIYLISRMPSDPQSGDIVPIVLISL
                     ALAFVATIYPSWRASRVQPAEALRYE"
     gene            8424..9119
                     /locus_tag="PKV56_11475"
     CDS             8424..9119
                     /locus_tag="PKV56_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX68477.1"
                     /translation="MSEALAPAGSVIRAIGLHKRFHEGALDVQVLQGVDLDIAAGETV
                     AIVGASGSGKSTLLHLMGGLEAPTQGQVLLMGRDLMGMDAQAQGVWRNRHLGFVYQFH
                     HLLPEFTALDNVAMPLRIRRVPQPEARAAAEAVLQQVGLGHRIGHRPAQLSGGERQRV
                     AIARSLAGRPACVLADEPTGNLDRETADGVFELMLSLARERGMAFVLVTHDEALAARC
                     DRVLRLKLGRLVP"
     gene            complement(9141..9776)
                     /gene="yfcF"
                     /locus_tag="PKV56_11480"
     CDS             complement(9141..9776)
                     /gene="yfcF"
                     /locus_tag="PKV56_11480"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HOX68478.1"
                     /translation="MALNTFTLHVDHRYMSPYAMSAFVTLVEKGIDFELVEIDLSAGQ
                     HRASDYQARSSTGRVPMLSHGDFDLSESSAISEYLEELFPAPGHVAVYPEHIADRARA
                     RQIQAWLRSDLMPLREERPTTVIFDQPIYTALSPAARAAADRLCAAATEWLGHEADQL
                     FGAWSIADTDLALMLNRLVINGDAVPESLARYAHRQWQRPSVQRWLALSRR"
     gene            complement(9830..10903)
                     /locus_tag="PKV56_11485"
     CDS             complement(9830..10903)
                     /locus_tag="PKV56_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX68479.1"
                     /translation="MNFLWPDMLWLLLWLPALVVVYMWLLARRKKTALRYTSLSLVRQ
                     AAGSGPGWRRHLPPLLMLLALAALLLATARPVAVVMLPSQQETVILAMDVSGSMRAAD
                     VQPNRLVAAQEAAKAFVAELPRSVRVGIVSFAGTAAVVQPPTHNRADAIAAIDRFQLQ
                     RGTAIGSGIVLSLATLFPEAGIDLSQVTGARAMPPATASTQGLPPAPVPGGSADKPAP
                     DFTPVAPGSYGSGAIILLTDGQRTVGPDPIESARMAADRGVKVYSVGIGTRDGDSISF
                     EGWTMRVKLDEDTLRSISTVTMGEYFYAGNAADLKKVYQSLSTRLVVEQKETEVGSLF
                     SGAAAVLLMLSVALSVAWFGRVA"
     gene            complement(10900..11889)
                     /locus_tag="PKV56_11490"
     CDS             complement(10900..11889)
                     /locus_tag="PKV56_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX68480.1"
                     /translation="MPEGASTEALLRQLEWTVIRRLDGLLQGEHRTLMRGAGFDLADL
                     REYQHHDDVRHIDWNVTARLQVPHVRQFTEDRELTVWFVLDLSASLDFGSVGNSSADG
                     RESVRTKRAVSAEFTGLMARLFTRHGNKVGALLYGNAVDAQLPPRASRQHVLHLMQKV
                     REHAVAPSGAQTQLRDLLQAVHKRIRRRSLVFVISDFISTPGWGDDMARLAQQHEVLA
                     VRLHDPAELDLPDVGLMSIQDAETGEQLLVDTHDAGFRRRFIAAAGRREAALRADLAH
                     AGVDALEIATDDGLAETVMRYADLRRQRSRLSSAGALPAHLGPVIKASAGADA"
     gene            complement(11882..12889)
                     /locus_tag="PKV56_11495"
     CDS             complement(11882..12889)
                     /locus_tag="PKV56_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX68481.1"
                     /translation="MDTSAPPTSTATLMQRVLYEVKRVVVGQDRFLERVMVAMLAQGH
                     LLVEGVPGLAKTITIKTLATTLRGSFKRIQFTPDLVPADLIGTRIYNQKTGDFSTALG
                     PVFANLLLADEINRAPAKVQSALLEVMQERQVTIAGETHKVPSPFLVMATQNPIESEG
                     TYPLPEAQVDRFMMKVLVGYPTDEEEFVIVQRVTGDPVAVSPVASTTQLAELQDECRK
                     VYVDPSLIAYAVKLVGSTRDPARHGLPELARYLNFGASPRATIGLIEASRAMAMLRGR
                     NYVLPDDLGDLVPDVLRHRLALSYEALADELTADAIIDRIVAKVPRPQPPMGQPGIAH
                     A"
     gene            complement(12922..13881)
                     /locus_tag="PKV56_11500"
     CDS             complement(12922..13881)
                     /locus_tag="PKV56_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX68482.1"
                     /translation="MKRAPLYSRSRRPALAADRAVESPAPASTSAPTAETADGTRLAP
                     RRHAVIVRLGGPFWAVCGALAVGAALLIQQAGFPPARQLSQADIDAAVRSSIEKQPLP
                     SAYATAYAAIRPSVVKVIGSDSDSEEANGSAQRDSPEAVQRSTGTGVVIVENGTILTN
                     LHVVSGSKRLRVVFADGLESEAVVVSTQPENDLAVLRARRIPDDLVAATLGSSSQLQP
                     GDQVLAIGYPFGIGPSASGGIVSGLKREFQADDDGKRMTNLIQFDAAANPGNSGGPLI
                     AMDGSVVGIVTAILNPGEAGTFIGIGFAVPIENAAGAAGMPPF"
     gene            13992..14813
                     /locus_tag="PKV56_11505"
     CDS             13992..14813
                     /locus_tag="PKV56_11505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX68483.1"
                     /translation="MRWIDTHCHLDAPEFAGGAAAVVGRARAAGVEQMVLPAVEAANF
                     ETVRLLAHKHDLFYALGIHPLYVERAAEGDVDQLAQALAAHREDPRLVAVGEIGLDHF
                     VAGSDRARQACFYAVQLQLARRFDLPVILHVRRSADHLLKQLRITPVRGGIAHAFNGS
                     EQQARAFVALGFKLGFGGALTFERALQLRRLAKTLPMEAIVLETDAPDIPPQWLYRTA
                     AERLASSHQAAPNEPSQLPRIAETLAALRGATPAEIAEATWANAHEALPGLRALA"
     gene            14810..15340
                     /locus_tag="PKV56_11510"
     CDS             14810..15340
                     /locus_tag="PKV56_11510"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOX68484.1"
                     /translation="MTTGAGDDPRPATDLLVGLPPVLAPHTRLVVLGSFPGVASLRAQ
                     QYYAHPRNQFWPLLSALWDIDLVGLPYAQRLAETSARGLGLWDVYASCRRAGSLDSAI
                     RDAVFNDLSSLKLRAPQLHAIAHNGGESARAMRHTQSLGVDVIRLPSSSPAHASWSFE
                     RKLAAWRAVFVGCGLA"
     gene            15567..16004
                     /locus_tag="PKV56_11515"
     CDS             15567..16004
                     /locus_tag="PKV56_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOX68485.1"
                     /translation="MTKLAMAAVAAGAMAGEPAEAAKMVKRDDASLSAMLVGTWVNPP
                     DSADYEGIPSAEVFNADGTYSYIEYEDKECQRVSGAVHSKWYVWDGTIVNEVGEGKTL
                     KDEIVNMAPKKMVLRSLEDSLTYHRVRSTAKTRACAGTPPQPK"
     gene            complement(16081..>16784)
                     /locus_tag="PKV56_11520"
     CDS             complement(16081..>16784)
                     /locus_tag="PKV56_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX68486.1"
                     /translation="QSEGGSLFLDEIGDMPLQLQTRFLRVLSENEVLPLGADHPIPVN
                     CTVIAATHRDLRDQVEAGTFREDLYYRLCGATLSLPALRDRADQRYLIEAIFQDEARR
                     LNSRSTLSSEIVDRLMGHQWPGNVRELRSALRFAIALSDDAPVTMADLPDEVVHGQHA
                     ERPRPSCIQPRTLGEDSTDSVAPDTDADSPARLLNALKRHKWNVTRAADELSLCRATI
                     YRQMKRFGITPPNQL"
BASE COUNT         2610 a         5575 c         5840 g         2759 t
ORIGIN      
        1 gctgccgcgg tgccaggcgc tgaggtccgg atcgagggaa aggtcgctgc gatgccacag
       61 aacctgagac gcttccatgt cggggatcga ctctccgaga tggccatctt ccacaacgtg
      121 gtctacctcg ccgggcaggt gcccagcgac acgcggcagg acatggccgg tcagacccgc
      181 caggttctcg cgatggtcga ccgcctgctg gccgaggcag gcaccgacaa cgcccacatc
      241 ctgatggcga ccatctatct tgccgacatg gccgatttcg atgcgatgaa tctcgtgtgg
      301 gatgactggg tcgcgcccgg cgacgcgccg ccgagagcga cggtgcaggc gcgtcttgcg
      361 cggcccgaat ggaagatcga gatcgtcgtg accgccgcgt tgtcggcggg tgcctgacgg
      421 tggcagctca tcgcatgatc cgggtgctgc tgatagccgc agccgcctat gtgctgctgg
      481 tggcgatgca gggtcgcacc gacaagcagg ccggcgcggc gctcgctgcg ctggcgaagc
      541 caggcgacat cgtcatgctg tcgtccgaga cctgcatgca ctgcgaccag gctcggcgct
      601 ggctcaccga gtacggtgtg ccgttccggg agtgtttcat cgagagggat gcggcctgtg
      661 ccgacagcta tcgagcgcag caatctcccg gcacgcccac gctgctggtg cggggtgagc
      721 gtcaggttgg tttcgatccc ggcctggtca cgaaggccat cagcggcggt tgagtcgtcg
      781 ccgtgatccg aggctagtcg gcatgcagac ccagcgcggc cgggtcgcca cggcccacgc
      841 gtaccaggac gggcgtgtcc ccggtcaggt cgaccaccgt cgttggctgc gccgcgcagg
      901 ctccggcatc caccaccgcc tgcagcactt tctggtagcg ctcgcggatg tcgtccgggt
      961 cgttcagcgg ctcggtttcg tcgcgcggga tcagcgtggt cgccaccagc gcctcgccgt
     1021 acaccgccag cagttcctgc aaggccttgt gttcgggcac gcgcaggccg atggtgcgcc
     1081 gagaagggtg cgagatgcgt cgaggcacct ccttggaggc ttcgaggatg aacgtgaacg
     1141 gcccgggcgt gccgagtttc agcaggcggt actggcggtt gtcgactcgt gcgtagctcg
     1201 cgagttcgct caggtcgcgg cacagcagcg tcaggtggtg cttgtcgtcg acgccgcgga
     1261 tgcgccgcag gttttccacc gccgacttgt cgtcgagccg gcacaccagc gcgtagctcg
     1321 aatcggttgg cacggccgcc acgccgccgt cgtggaggat ctgcgcagcc tgtttcagaa
     1381 ggcgaggctg ggggttgtcg gggtgtagtt cgaaatgctg ggacatcgcg cgattgtcgg
     1441 cgccgtgggc gcctggctct acgagacctg tccgggccgg cagctcaggc cggcagcagc
     1501 acccgttccg ccagcgcttc ccacaccggc gtcaggcgtg ccggcagtgc cgccagggtg
     1561 ccgaggtcgg tgcggctttc ctccgggctg tggaagtcgc tgccgcgcga ggccagcagg
     1621 tcgaattcga tggccagggt ggcgtacttg tcgtagtcgg cgctggtgtg gcttccggtg
     1681 accacctcga cccctcgtcc tccgtgtccc ttgaactcgg tgaacaacgc gtattccgcg
     1741 ttgacgctga acttgtagcg accgggatgg gcgatcacgg cgatgccgtc ggcctgggtg
     1801 atccacttca ccgcgttccc cagcgatgcc cagcggtgtt cgacgaaacc cggtttgcct
     1861 tcgacgaggt acttgcggaa cacctcgtgg gtgctggcgc acacgccggt ttcgacaagg
     1921 tagcgggcaa agtgggtacg cgctatcagt tcagggttgc ccgcgtagtt caacgcaccc
     1981 tcgaaggcat ctcggatgcc gaccttggcc agcccgtcgg ccatctcgtg tgcacgtgcg
     2041 atgcgtccgt cgcgggtatc cgccagtccc ttgatcaggg ccgcgttctc cggatcgatc
     2101 ccgagcccga cgatgtgcac cgtgagaccg gcgaaggtga ctgaaatctc cacgcccggc
     2161 aggtaaggca ggccgacagc ttcggctgca tcgctcgccc gccgctggcc gcccacctcg
     2221 tcgtgatccg tcagcgccca cagctcgacg ccgttggcct tggctcgttg ggccagtgct
     2281 tcgggcgtca gcgtgccgtc cgacacgcag gaatggcagt gcaggtcggc gttgatccag
     2341 gcggtaggga ctttcaccag ttgattgtag ggaaggggta catcacgagt cgccttgcat
     2401 ccgacgcgcc gaaggggcat cggtattggt tcacgaaccc cgtgtggtgg agtcctcagc
     2461 gcacgacgcg gatcagcttg ccactgtctg tcagcaggta gagccatccg tccgggcctt
     2521 gccggacgca ccggatgcgc tcgccgaggc cggcgaacat cctttcgcgc gacacctcgg
     2581 ccgtgccgtt cagcacgacg cgccagagtg cggtgccggc aagcgcgccc aggaaggcgt
     2641 tgccctgcca ttcggggaat ccggcacccg tgtagaacgc cagtccggaa ggcgcaatcg
     2701 aggtcgggac ccagaaagag acgggttcgg cgtacgtcgg cgcgtgcgtg cccccgccga
     2761 tccggcaggc ctcgccgacc gggtcaccgt agttgcatcc gtagctcttg accggccagc
     2821 catggttggc accggccgcc acccggttca actcgtctcc gccctgcggc ccgtgctcga
     2881 ccagccacag ttcgccggtc gtcgggtgca gcgcggcacc ctgcgcattg cgatggccca
     2941 ggctccagat ctcgggtcgt gccgtcgcgc ccagatcggg attgcctgcg ggcacgctgc
     3001 cgtcgcgagc gatgcgcacc accttgccca ggtgcgttga caggtcttgc gccggactgc
     3061 ctttctgccg ttctccgagg gtcacgaaca aggtcttgtc gccgcgaaac acgagacgcg
     3121 acccgaagtg gccgttgccg accaccttcg gcagttgccg gaagatcacc tggacgtcgg
     3181 tcagcgccag gcccgaaagc cgcccccgcg ccaccgcagt gccgctgccg gccaggccgg
     3241 cctcggcata ggaccagtag acccaggggt cggtcgtgaa atcggggtct gtggccacat
     3301 cgagcaggcc gccttgcccg gccgaggcga ccgggggcac gccggtcaag gccgccgaca
     3361 cggtcgcgcc atccgcgctc acgatgacca tcgatccgcc cttctgggtg accaccatcc
     3421 ggccatcggg caggaacgcc aggccccacg gcgacgaaag ggctcgggtc agcacgacag
     3481 cgcgagccgc cgtcgcgacc ggtgcaggcg gggtactcgc cgagccgcca ccgcacgccg
     3541 acgcgccgac gaggagaccc gcgcctgctg tcgccagcgc tgacagcttc cagccggctt
     3601 cgatcaactc tcggcgtctg gggttggcca gtgtgttgac gcatcggccg ggcgtcgcgg
     3661 ggtgtggcat gggggctcct gtcggctcgc tgccagtatg cggcgttcgg tcacgggtcg
     3721 accactgctc ttgcgttgcc agttgtgtct ggcccgggtc gtcgcgcgtc ccgccagatc
     3781 tgcttcggcc gtgctcacgc tcccgtggct tgggccgctt ccacgatcat ctgcagccgt
     3841 tggcggccgt tgtattcgtc gatgctcagg cgataggcca gcgtgacccg ggcgggcacg
     3901 gggtcgacat ggccgaacca gatcgcgtcg cgcaacgtgc ccgaatgctt cacgctgagc
     3961 ttcaggtgct tctcgccgac cagacgctgg ctgacgactt ccacttcgtc gctgaacagg
     4021 ggcgcttcga acgaagggcc ccacacctgc gcattcagag cctgcacggt gtccgtcgtg
     4081 aagtgctcga tcccgagtgg gccgtcgctg agcagccgcc gctccagcgt cgcggcatcc
     4141 agcccttcga gcgcggcctg ctgaaacgcg gcgtcgaagg tcgcgaaatc ggcttcggcc
     4201 agggtgcagc cggcagccat cgcgtgcccg ccgaacttct tcagcacacc ggggtggcgc
     4261 ttgctgacga ggtccagcgc gtcgcgcagg tggaagccct cgatcgaccg ccccgagccc
     4321 ttcagcagcc catcctggcc gcgtgcgaac acgaaggtcg gcctgtgaac ccggtccttc
     4381 agacgcgacg ccacgatgcc gaccacgcct tcgtggaagt cggggtcgta gatcgccagg
     4441 gccggtggcg ctgcgcccgg atcgttcatt ccctgcatca gcgtgtcgag cagtgcctcg
     4501 gcctgttcgc gcatgccggt ctcgacctcg cgtcgctcgc ggttgatcgc gtcgagctgc
     4561 tgcgcgagtt gcgcagcgcg tgccggatcg tcggtcagca ggcactcgat gcccagcgtc
     4621 atgtcggaca ggcgccccgc tgcgttgatg cgcgggccga gcgcgaagcc gaaatcgaaa
     4681 ccggcggcgc gcgaggggtc gcggccggcg gccgcgaaca acgcggccac gccggcctgc
     4741 atgcgtccgg tgcggatgcg cctgagcccc tgcgccacca gccgccggtt gttggcgtcg
     4801 agccgcacca catctgccac ggtaccgagc gcgaccagat cgagcagggg gtcgagcttg
     4861 ggttgcgccg gagccgagga cggaccgccc gaagcatcgg caaacaagcc gcgtgcgcgc
     4921 agctcgctgc gcagcgccat cagcacgtag aacatcacgc cgacgccagc caggtgcttg
     4981 cttgcgaaac cgcagcccgg ctggttcggg ttgacgatca catcggcatc cggcagcacg
     5041 atcgcatcgt cttcctgcgc cggcaggtga tggtcggtca cgacgacggc aagtccgtgc
     5101 gcccgcgcgt gggcgacgcc agccaggctc gcgatgccgt tgtcgacggt caccagcacc
     5161 tgcgcgccct gcgccagtgc caggtcgacg atcgctggcg tgaggccgta gccgtgcacg
     5221 gcgcggtcgg gcaccacgta gccgagcgtg cctggcgccg cgcccagcag agccagtccg
     5281 cgcagtgcga ccgcgcaggc cgtggccccg tcgcagtcgt aatcggcgac gatgcacagt
     5341 cgcaggcgtt cgtgcagcgc atcggccagc agcacggcgg cttcggctgc gccccgcagt
     5401 ccggccggcg gcagcaggcg ggcgagcccg tcgtcgagtt cctcgatgcc gcgcacgccg
     5461 cgagccgcga acagccgcgc cagcagcgga tgcacgccgg cctgttcgag cgcccaggcc
     5521 gagcgggggg ctgccgggcg cagcgtcagg gtcggggagt tcacagcgcg gtcagcgtgg
     5581 cgtgtggatc gacggtgcgg aagcggtcgc cgagtcgctg ccacagcgag cgctgcatcg
     5641 cgtcgtagcg ttgccagccg cgttcgccgc acagcgtcag cgacacccct tcgccacgat
     5701 cggctgcggc cacgagaggg cccagtgcgt cggcgtcgag ggcccgccag ctttcggccc
     5761 agccagccca atcctcggcc agcagcggcg cgcgcaggcg gtcgttcacc gtcacctcgg
     5821 cggaggcgga ctgcgcgcgg ccgcagccgc tgagccagaa cgagttgacc ggcagcgcgc
     5881 cgcgggcttc gcgggcctcg ttgaccgggt gctggtgcag cagcatctgc acctcgttct
     5941 gcagccgccg cacccagcga acccgcgcca gctgttcgac ggacgcttgc gggtcgccgc
     6001 gcagccacag atcgacgttg cggccgatca cgcgatcgat cgacgcgcag ggcagatcgg
     6061 ccagctgctc gtgcgagagg taccagcgat cgggcgccgc ccaggcgagc gatccgtcgt
     6121 cgtcatcgaa cagcggccgc accgcgtcga acagcgtgcg cgactcgtcg gcgggcaggg
     6181 cgagtgccag cggatcggcc agcgtgatgt gttcgcgtcc gacatgccag tgcaccggcg
     6241 tcacctggcc ccacggacgc ctcccgacat cgatgccgtc ggcacgggcc tgcagcgcgg
     6301 cccagggcca tcgcccatcg gcgccgtgcc agccctgtgc cgtcgcgagc gcgcgctcgt
     6361 gcggcggcga cagcgtgtac tcgtcgtcga catcgcgggt ggtgggctgc agccgcgtca
     6421 gcaatcgggc caggttcggc agggcgagat cacgcagcgt gtgggcgctg gcctcggaga
     6481 cggcgctggc gtgggggatg agcaggtgca tatctgctct attgtccggc ggcggccggg
     6541 gcgcttttgc ttgcctggca tgggtgggtg cgttgcgttg cagccagccg gggtttcggc
     6601 ccggcggccg agtcactttc ttctgctgcc ccagaagaaa gtaaccaaag aagaggggcc
     6661 caacaccagc catttgatct gttcgcttcg gctgcacacc tacttcaagg gctctggccc
     6721 gagaaccgaa accggactag cgcctcggtt tcctcgatca cattcagatg actcggcatc
     6781 gacacgactc gaccctgcgg gtcatcgccg cgaggacctg cagggtcgag ccgaagcgag
     6841 aacaagcgga ctggcattag cgaggaagcc gaggcgctag tccggtttcg gttctcgtgc
     6901 cggagcctct gccgtcagcc ttctggccgc agcgtgacgg ccaaatcggc tggtgttcag
     6961 gccctttgct ttggtgactt tcatttgggc ccgcaaatga aagttactcg gctgccgggc
     7021 cgagacccgg cgggttgcag cgcctcggca caacctcccg acgggtcgga catccgacaa
     7081 ggtctatcga agaatggcca acgtcgccgg cgagatcgaa tcgcagcgat ctacatcgca
     7141 atgcactcaa cccctcctgt agcatccgcc gtcaatgcaa tggccctacg aactccagat
     7201 cggctggcgc tacacccgcg ccgggcgcag cgggcggcgc aacgggttca tctcgttcat
     7261 ctccggcgtg tcgatgctcg gcatcgcgct cggtgtggcg gcgctgatca tcgtgctgtc
     7321 cgtgatgaac ggcttccaga aggaagtgcg cgaccgcatg ctgtcggtga tctcgcacgt
     7381 cgaggtgtac cagtcgaagg gtgaggccct gcccgactgg caggcgacgg ccgcacaggc
     7441 ccggctcagc ccgcaggtcg tcggcgccgc gcccttcatc gctgctcagg cgctgatcgc
     7501 gcgcggtgac gacatgcgtg gtgccgtcgt gcgcggcatc tcaccgatcg aggaagcgac
     7561 cgtcaccgaa ctcgccgcca agctgcgcga caccacgctg gccaggttgg tcccgggcgg
     7621 ctggggcgtc gtgctgggcg gcgagctggc gcggggactg ggtgtgaggg tgggggacaa
     7681 ggtgaccatc gtcgctcccg gagggcaggt cactcccgcc ggggtcgtgc cccgactgaa
     7741 gcagttgacc ctggtcggta ccttcgaggc cggccactac gagtacgaca acgggctggc
     7801 gctgatccac gtcgaggacg cggcgaggct gttccgcgtc gaagggccga ccggcgtgca
     7861 gcttcgcctg aaggacgcac agcaggcgcg cgccgtggcg gacgaactgc aggtgcgact
     7921 cgggccgagc gtgcgcctga gcgactggac ccgaaccaac cgcaactggt tccaggcggt
     7981 ccagatcgag aagcggctga tgttcatcat cctgacgctg atcgtcgcgg tcgcggcatt
     8041 caacctggtg tccacgctgg tgatgacggt gaccgacaag caggccgaca tcgcgatcct
     8101 gcgcacgctg ggtgcgagcc cccgttcgat catggcgatc ttcatggtcc agggtgcggc
     8161 ttccggcgtg atcgggacgc tggccggcat ggtgctcggg ctggcggtgg cgttcaacat
     8221 cggcgtcatc gtgccggcga tcgagcgcgc gctgcacatg agcttcctgc cgagcagcat
     8281 ctacctgatc agccgcatgc cgagcgatcc gcaaagcggc gacatcgtgc cgatcgtgct
     8341 gatctcgctg gcgctggcct tcgtcgcgac gatctacccg agctggcgcg cgagccgcgt
     8401 gcagccggcc gaggcgctgc gctatgagtg aagccctggc gcccgccggc agcgtgatcc
     8461 gcgcgatcgg actgcacaag cgcttccatg aaggcgcgct cgacgtgcag gtgctgcagg
     8521 gcgtggacct cgacatcgcg gccggtgaaa cggttgccat cgtcggcgcg tcgggctcgg
     8581 gcaagagcac gctgctccac ctgatgggcg ggctcgaagc gccgacgcag ggacaggtgc
     8641 tgctgatggg ccgcgacctg atgggcatgg acgcacaggc gcagggcgtc tggcgaaacc
     8701 gccacctggg cttcgtctac cagttccatc acctgctgcc cgagttcacc gcgctcgaca
     8761 acgtcgcgat gccgctgcgc atccgccgtg tgccgcagcc cgaggcgcgc gcggccgccg
     8821 aggccgtgct gcagcaggtc ggcctcgggc accgcatcgg tcaccggccg gcgcagttgt
     8881 cgggtggtga acgtcagcgg gtggcgatcg cgcgctcgct ggccggacga cccgcctgcg
     8941 tgctggccga tgagccgacg ggcaacctgg accgcgaaac cgccgacggc gtgttcgagc
     9001 tgatgctgtc gctggctcgc gaacggggca tggccttcgt gctggtcacc cacgacgagg
     9061 cgctggctgc ccgttgcgat cgggtgctgc gactgaagct ggggcggctg gttccctgag
     9121 ccgcccacgc cagccgaccg tcacctgcgc gacagtgcca gccagcgttg caccgagggg
     9181 cgttgccact gccggtgcgc atagcgggcc agcgattcgg gcaccgcatc gccgttgatc
     9241 accaggcggt tgagcatcag cgccaggtcg gtgtcggcga tcgaccaggc accgaaaagt
     9301 tgatcggcct cgtggcccag ccactcggtc gccgcggcgc acaggcggtc ggcagcggca
     9361 cgcgcggcgg gcgacagggc ggtgtagatc ggctgatcga agatgacggt ggtcggccgc
     9421 tcctcgcgca agggcatcag gtcgctgcgc agccaggcct ggatctggcg agcacgggcg
     9481 cggtctgcga tgtgttccgg gtagaccgcg acatggccgg gcgccggaaa cagttcctcg
     9541 aggtactcgg agatggcgga cgattcggac aggtcgaagt ctccgtgaga cagcatcggg
     9601 acccggccgg tggacgagcg cgcctggtag tcgctcgcgc gatgctgccc cgccgacagg
     9661 tcgatctcga cgagttcgaa gtcgatgccc ttttcgacca gcgtcacgaa cgccgacatc
     9721 gcgtacgggc tcatgtagcg atggtcgacg tggagggtga aggtgttgag cgccatggtg
     9781 cggggtgtcc tgaatcggag ggtattcggg ttacgcggtg cgagggtggt caggcgacgc
     9841 ggccgaacca ggccaccgac agcgccaccg agagcatcag aagcaccgcg gctgcgccag
     9901 agaacaggct accgacctcg gtctccttct gttcgaccac gaggcgggtg ctgagcgact
     9961 ggtagacctt cttcaggtcg gccgcgttgc cggcatagaa atactcgccc atcgtcaccg
    10021 tgctgatgct cctgagcgtg tcctcgtcga gcttcacgcg catcgtccag ccttcgaagc
    10081 tgatgctgtc gccgtcgcgc gtgccgatgc ccaccgaata caccttgacg cctcggtcgg
    10141 cagccatccg cgcggactcg atcggatccg gacccaccgt gcgctgtccg tcggtcagca
    10201 ggatgatggc gcccgagcca taactgccgg gtgccacggg ggtgaagtcg ggtgccggct
    10261 tgtcggcact gcccccgggc accggtgcag gcggcaggcc ttgcgtcgat gcggttgctg
    10321 gcggcatggc gcgggcgccg gtcacctgcg acaggtcgat gccggcctcg gggaacagcg
    10381 tggccagcga cagcacgatg ccactgccga tcgcggttcc gcgctgcagt tgaaagcggt
    10441 cgatcgcagc gatcgcgtcg gcccggttgt gggtcggcgg ctgcaccacg gccgccgtgc
    10501 ctgcaaacga cacgatgccc acgcggaccg atcgcggcaa ctcggcgacg aacgccttgg
    10561 ctgcttcctg ggcagcaacc agacggttgg gctggacatc ggctgcgcgc atgctgcccg
    10621 agacatccat cgcgagaatg acggtctcct gctggcttgg aagcatgacg acggccaccg
    10681 gtcgcgcagt cgccagcaac agcgctgcca aggccagcag catcagcagc ggcggcaggt
    10741 ggcgccgcca gccggggccg ctgccagcgg cctgcctgac cagcgacagg ctggtgtagc
    10801 gaagcgcggt cttcttgcgc ctggccagca gccacatgta gacgaccacc agggccggca
    10861 gccacagcag cagccacagc atgtcgggcc acaggaaatt catgcgtccg ctccggcgga
    10921 ggctttgatc acgggcccca ggtgcgcggg cagcgcgccg gccgacgaca gccggctgcg
    10981 ttgccggcgc aggtcggcgt agcgcatcac cgtctcggcc aggccgtcat ccgtggcgat
    11041 ctcgagggca tcgacgccgg catgcgccag gtctgcgcgc agggctgcct cgcgccgtcc
    11101 tgcggcagcg atgaagcggc ggcgaaagcc tgcatcgtgc gtgtcgacca gcaactgttc
    11161 gccggtttcg gcgtcctgga tcgacatcag gccgacgtcg ggcaggtcga gctcggccgg
    11221 gtcgtgcaat cgcaccgcca gcacctcgtg ctgctgcgcg aggcgcgcca tgtcgtcccc
    11281 ccagcctggc gtgctgatga agtcggagat cacgaacacc agggaccggc ggcgaattcg
    11341 cttgtgcacc gcctgcagca ggtcgcgcag ctgcgtctgt gcaccggagg gcgccaccgc
    11401 atgttcgcgc accttctgca tcaggtggag cacatgctgc cggctggcgc gtggcggcag
    11461 ttgagcatcg accgcgttgc cgtacagcag tgcaccgacc ttgttgccgt ggcgggtgaa
    11521 caggcgtgcc atcaggccgg tgaattcggc cgacaccgcg cgcttggttc gcaccgattc
    11581 acgcccgtct gcagacgagt tgccgacact gccgaagtcg aggctggcac tcaggtcgag
    11641 cacgaaccag accgtgagct cgcggtcttc ggtgaattgc ctcacgtgtg gcacctgcaa
    11701 tcgcgcggtg acgttccagt cgatgtggcg cacgtcatcg tggtgctggt actcgcggag
    11761 gtcggcgaga tcgaagcccg cgccgcgcat cagcgtgcgg tgctcgcctt gcagcaggcc
    11821 atcgaggcgg cggatcaccg tccattccag ctggcgcagc agggcttcgg tgctcgcgcc
    11881 ctcaggcatg tgcgatgccc ggctgcccca tcggtggctg cggccgcgga accttcgcga
    11941 cgatgcgatc gatgatcgcg tcagccgtca attcatcggc cagcgcctcg tagctcagcg
    12001 ccagtcgatg gcgcagcaca tcgggcacga gatcgcccag gtcgtctggc agcacgtagt
    12061 tgcggccgcg cagcatcgcc atcgcgcgag acgcctcgat caggccgatg gtcgctcgcg
    12121 ggctggcacc gaaattcagg tacctggcca gctcgggcag gccgtgccgg gccgggtcgc
    12181 gcgtcgagcc caccagcttg actgcgtagg cgatgagcga cgggtcgacg tacaccttgc
    12241 ggcactcgtc ctgcaactcg gccagctggg tggtgctggc caccggggac acggcaaccg
    12301 ggtcgccggt gacccgttgc acgatgacga actcttcctc gtcggtcggg tagccgacca
    12361 gcaccttcat catgaagcgg tcgacctggg cctcgggcag cgggtaggtg ccctcggact
    12421 cgatcgggtt ctgcgtggcc atcacgagga atggcgacgg caccttgtgg gtttctccgg
    12481 cgatcgtgac ctggcgctcc tgcatcacct cgagcagcgc gctctgcacc ttggcgggcg
    12541 cccggttgat ctcgtcggcc agcagcaggt tggcgaaaac cggccccagc gcggtgctga
    12601 aatcgccggt tttctggttg tagatgcgcg tgccgatcag atcggccgga acgagatcag
    12661 gcgtgaactg gatgcgcttg aagctgccgc gcagcgtggt ggccagcgtc ttgatcgtga
    12721 tcgtcttggc cagtcccggc acgccttcga ccagcagatg gccctgcgcc agcatcgcga
    12781 ccatgacccg ctcgaggaag cggtcctgcc ccacgacgac gcgcttgact tcgtagagca
    12841 cgcgctgcat cagcgtggcg gtgctggtcg ggggggctga tgtgtccatg ggcgggctcg
    12901 tggtgtggct gcagtgcgcg ttcagaaagg cggcatgccg gccgcgccgg ctgcgttctc
    12961 gatcggcacc gcgaatccga tgccgatgaa ggtgccggcc tcgcccgggt tcaggatcgc
    13021 ggtgacgatg ccgaccacgc tgccatccat ggcgatcagg ggcccgcccg agttgcccgg
    13081 gttggcggcc gcgtcgaact ggatgaggtt cgtcatccgc ttgccgtcgt cgtcggcctg
    13141 gaactcccgc ttcagtcccg acacgatccc tcccgaagcc gacggaccga tgccgaacgg
    13201 ataaccgatg gccagcacct gatcgcccgg ttgaagctgc gacgacgaac cgagtgtcgc
    13261 cgccaccagg tcgtccggga tgcggcgcgc acgcagcacc gcgaggtcgt tttccggctg
    13321 cgtgctgacg acgacggcct cggactccag cccgtcggcg aacacgactc gcaaacgctt
    13381 cgatcccgac accacgtgca ggttggtcaa gatcgtcccg ttctcgacga tcaccacgcc
    13441 ggtgccggtc gagcgctgca ctgcctccgg gctgtcgcgc tgggctgacc cgttggcctc
    13501 ttcggaatcg gaatcggacc cgatgacctt gaccacggac ggccggatcg cggcataggc
    13561 ggtcgcgtac gcggagggca gaggttgctt ttcgatgctg ctgcgcacgg cggcatcgat
    13621 gtctgcctgg ctgagctggc gcgcgggcgg gaagccggcc tgctggatga gcagcgcggc
    13681 cccgactgcg agggcgccgc acacggccca gaagggtcca cccaggcgca cgatgacggc
    13741 atgtcttcgc ggcgcaaggc gggtgccatc ggccgtttcg gctgtggggg cggaggtgga
    13801 ggcgggcgcc ggtgattcca cggctcgatc ggctgccagc gcgggtcggc gcgaacggct
    13861 gtaaagcgga gccctcttca ttgctgcact cccgacgtgg ctgatccgac cctggtgggg
    13921 cgaaccgtat cacgggcgat tgcgcagtgc acggtgcgag gcaccgagtc gcaccaaggc
    13981 atcatggacg catgcgctgg atcgataccc actgccatct cgatgccccc gaatttgccg
    14041 gaggtgcggc cgccgtggtc ggccgggcgc gtgctgcggg cgtcgaacag atggtcctgc
    14101 ctgcggtcga ggcggccaac ttcgagacgg tgcgcctgct cgcccacaaa cacgacttgt
    14161 tctatgcgct gggtatccat cccctgtacg tcgaacgtgc tgccgagggc gacgtggatc
    14221 aactcgcaca ggcgcttgcc gcacatcgcg aggacccgcg cctggtggcg gtcggagaga
    14281 tcgggctgga tcatttcgtc gcaggttccg atcgtgcgcg acaggcctgc ttctatgcgg
    14341 tgcaattgca actggcgcga aggttcgatc tcccggtgat cctgcatgtc aggcgctcgg
    14401 ccgaccattt gctgaagcag ctgcgcatca cgccggtgcg cggcggcatc gcgcatgcgt
    14461 tcaacggcag cgaacagcag gcgcgcgcct tcgtcgcact gggcttcaag ctgggattcg
    14521 gtggtgcgtt gacgttcgag cgcgcgttgc agcttcggcg cctggcgaag accttgccga
    14581 tggaggcgat cgtgctggaa accgatgcgc cggacattcc tccgcagtgg ctgtaccgaa
    14641 ctgccgccga gcgactcgcc tcttcgcatc aggcagcgcc caacgagccc tcgcaactgc
    14701 cgcgcatcgc cgagacgctg gccgcgctgc gcggagcgac gccggccgag atcgcggaag
    14761 ccacctgggc caacgcgcac gaggcgttgc ctgggctgcg ggccctcgca tgacaacggg
    14821 cgcaggggac gatccgcggc cggcgaccga tctgctcgtc ggcctgccgc cggttctggc
    14881 gccgcacacc cggctggtgg tgctcggcag ctttccgggt gtcgcgtcac tgcgcgcgca
    14941 gcagtactac gcccatccgc gcaaccagtt ctggccattg ctgtctgcac tttgggacat
    15001 cgatctggtc ggcctgccgt atgcgcagcg tctcgccgag acgagcgcac gcggattggg
    15061 cctgtgggac gtctatgcca gctgccgccg ggcgggcagt ctcgacagcg cgatccgcga
    15121 tgcggtattc aacgacctgt ccagcctgaa gctgcgggcc ccgcagctgc acgccattgc
    15181 acacaacggg ggcgagtcgg cgcgggcgat gcgccacacg cagtcgctgg gagtggacgt
    15241 gatccggctt ccctcgagca gcccggccca tgccagctgg agtttcgaac gcaagctggc
    15301 agcgtggcgt gccgtgttcg tcggttgcgg cctggcctga gcgtcccggc ctctgcatag
    15361 gggttacccc gagtcgcgtg tgggcggccc ggaaagcggt acggagaaag cgtgttcgtg
    15421 catccaggag tcgaggttct acgtaacatt gaggccgggg cgatgtcgtg tccttgactg
    15481 ggaagtgccc cagtcccatc gactgtccat ggccaccggc gattccagat cacgagacgc
    15541 ccatcacttc acaaggaaat gctttgatga caaaactcgc aatggcagca gtagccgccg
    15601 gtgccatggc cggggaaccc gccgaggcgg ccaagatggt caagagggac gacgcttcgt
    15661 tgtccgccat gctggtcggc acctgggtca acccacccga cagcgccgac tacgaaggca
    15721 tcccgagtgc ggaggtgttc aacgcggatg gcacgtattc ctacatcgaa tacgaggaca
    15781 aggaatgcca gagggtgtcg ggcgccgtcc actctaagtg gtacgtgtgg gatggcacga
    15841 tcgtcaatga agtcggcgaa ggcaagaccc tgaaggacga gatcgtcaac atggcgccga
    15901 agaagatggt gttgcgatcg cttgaagaca gcctgaccta tcaccgagtt cgctcgacgg
    15961 ccaagacccg cgcatgcgcg ggcaccccgc cgcagccgaa ataggtcgat tcggctgcag
    16021 tcgatcgaga cgactggagc catcccgaag gcccgcgctc cggcacgggc cttcaaccgg
    16081 tcaaagctgg ttcggtgggg tgatcccgaa acgcttcatc tgcctgtaga tggtggcccg
    16141 gcaaagggac aactcgtcgg cggcccgcgt cacattccat ttgtggcgct tcagggcatt
    16201 cagcaatctc gcgggcgagt cggcgtcggt atccggcgcc acggaatccg tcgaatcctc
    16261 gcccagggtg cggggctgga tgcaggacgg ccgcgggcgc tcggcatgct gcccgtggac
    16321 gacttcgtcg ggcaggtcgg ccatcgtcac aggtgcatcg tcgctgagcg cgatggcgaa
    16381 gcgcaacgcg ctgcgcaatt cgcgcacgtt gccaggccat tggtgtccca tcaggcggtc
    16441 gacgatttcc gaggacagtg tcgagcgcga attcaatcgg cgcgcctcgt cctggaagat
    16501 cgcctcgatc aggtagcgtt gatccgcacg gtcgcgcagt gccggcaggc tgagcgtggc
    16561 tccgcacaag cggtagtaga gatcctcccg gaacgtgccg gcttccacct ggtcgcgcag
    16621 atcgcggtgt gtggccgcga tgaccgtgca attcaccggg atcgggtgat cagcgcccag
    16681 tggcagcacc tcgttctcgg acagcaccct caggaagcgg gtctgcagtt gcaatggcat
    16741 gtcgcctatt tcgtcgagaa acagcgagcc accctcggac tgca
//
LOCUS       DAOORV010000080        16462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4293_length_16462_cov_8.303041, whole genome shotgun sequence.
ACCESSION   DAOORV010000080 DAOORV010000000
VERSION     DAOORV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_16462_cov_8.303041"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKV56_11525"
     CDS             complement(<1..760)
                     /locus_tag="PKV56_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOX68487.1"
                     /translation="MSAVATEGKIVQCIGAVVDVEFPRNAMPKIYDALKLDSLGLTLE
                     VQQQLGDGVVRTIALGSSDGLRRGLTVTNTQNPITVPVGRATLGRIMDVLGRPIDERG
                     PVDQELTASIHRKPPAYDELSPSQDLLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTV
                     NMMELINNIAKQHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVFGQMNEPPGN
                     RLRVALTGLTIAESFRDEGKDVLFFVDNIYRYTLA"
     gene            complement(817..1698)
                     /gene="atpG"
                     /locus_tag="PKV56_11530"
     CDS             complement(817..1698)
                     /gene="atpG"
                     /locus_tag="PKV56_11530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOX68488.1"
                     /translation="MAAGKEIRGKIKSVENTKKITKAMEMVAASKMRKAQDRMRAARP
                     YAEKIRSLAANLSKATPEYKHPFLVQNTGVKSVGFIVVTTDKGLCGGMNTNVLRAVTL
                     KLKEIEGQGLKAEAVAIGNKGLGFLNRIGAKVVSHATQLGDKPHLEKLIGPVKVLLDA
                     YAEGKLGAVYLCYTGFINTMKQEPLVQQLLPLSSKSMEVDTPAGAGKYGWDYLYEPDA
                     ETVIDELLLRYTEALVYQAVAENMASEQSARMVAMKSATDNAGSVIAELKLVYNKTRQ
                     AAITKELSEIVAGAAAV"
     gene            complement(1729..3282)
                     /gene="atpA"
                     /locus_tag="PKV56_11535"
     CDS             complement(1729..3282)
                     /gene="atpA"
                     /locus_tag="PKV56_11535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOX68489.1"
                     /translation="MQLNPAEISELIKSRIEGLGASTDIRNQGTVVSVADGICRVHGL
                     SDVMQGEMLEFPASPDGSSTFGLALNLERDSVGAVILGEYEHIAEGDTVKCTGRILEV
                     PVGPELKGRVVNALGQPIDGKGPINAQLSMPIEKIAPGVIARKSVDQPMQTGLKSIDS
                     MVPVGRGQRELIIGDRQTGKTAVAIDAIINQRGQNMTCIYVAIGQKASSVKNVVRALE
                     ANGAMEYTIVVAATAAESAAMQYVSAYSGCTMGEYFRDRGEDALIVYDDLSKQAVAYR
                     QVSLLLRRPPGREAYPGDVFYLHSRLLERAARVNADYVEAFTKGAVKGKTGSLTALPI
                     IETQAGDVSAFVPTNVISITDGQIFLETSLFNAGIRPAINAGISVSRVGGAAQTKVIK
                     SLSGGIRTDLAQYRELAAFAQFASDLDEATRKQLDRGARVTELLKQAQYSPLPISLMG
                     ATLFAVNKGFMDDVDVKKVLAFESGLHQFLKTSHAGLLKKIEDAKALDKDAEAELSSA
                     VAAFKKSFA"
     gene            complement(3374..3907)
                     /locus_tag="PKV56_11540"
     CDS             complement(3374..3907)
                     /locus_tag="PKV56_11540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOX68490.1"
                     /translation="MAELATLARPYAEALFDVASQGDVAAFAQQVDALAAIAGNAQLR
                     EFANSPKVSSEQVFDLIASVVKSPLSEPMKNLLRTVIDNGRLTVMPEIAQQFHALANA
                     GAGVSDATVFSAFPIEATQLADVVATLEKRFARKLNVVVQLEPALIGGIRVVVGDEVM
                     DTSVKARLEQMRVALTA"
     gene            complement(3940..4410)
                     /locus_tag="PKV56_11545"
     CDS             complement(3940..4410)
                     /locus_tag="PKV56_11545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOX68491.1"
                     /translation="MSITATFIIQMIVFLILVGFTMKYIWPPITAALDARAQKIADGL
                     SAADKAKAELTTANKRVEEQLSAARTDAAQRLADAERLAQSMVEEAKTRANEEGAKIV
                     SAAKAEAEQEAIKAREILREQVAALAVKGAEQILQREVNAGVHADLLNRLKVEL"
     gene            complement(4494..4760)
                     /gene="atpE"
                     /locus_tag="PKV56_11550"
     CDS             complement(4494..4760)
                     /gene="atpE"
                     /locus_tag="PKV56_11550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOX68492.1"
                     /translation="MMELIGLVAVAAGLIIGLGAMGACIGIGVMGSKYLESAARQPEL
                     MGELQTKMFLLVGLIDASFIIGTGIALWFTTANPFLAQIANLPK"
     gene            complement(4806..5666)
                     /gene="atpB"
                     /locus_tag="PKV56_11555"
     CDS             complement(4806..5666)
                     /gene="atpB"
                     /locus_tag="PKV56_11555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOX68493.1"
                     /translation="MSAEGHALTSGEYIVHHLTHLSTGKPASLVDFSVINFDSIVIST
                     LLGVLGCFLLWRAASKATAGVPGRFQAAVEILFEMVESQAKGIVHNAESRKLVAPLAL
                     TVFVWIFLLNAMDLLPVDLLPLIWEKIYVAAGGDPHHAFLRVVPTADLSITMGLSVGV
                     LITCLVYNVKIKGFGGWVHELFAAPFGNHWALYPFNFAMQIVEFVAKTVSHGMRLFGN
                     MYAGELIFLLIALLGGAFTLSLGGFALAALQILAGTVWAIFHILIITLQAFVFMMLTL
                     VYIGQAHETH"
     gene            complement(5694..6170)
                     /locus_tag="PKV56_11560"
     CDS             complement(5694..6170)
                     /locus_tag="PKV56_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOX68494.1"
                     /translation="MTQLIARGVASAGFDDEAEEPPVKPLTRDEAQALRVKSPQLSPW
                     RVIAAQAVVGCLMALGAWWFSDQPGFVQSAIYGSIVAVLPGALMARGMTSRLSSLSVG
                     SSAVSVMLWTVMKIGVSIAMLMLAPKLVQSLSWPVLLATLVLCMQVYLVALLWRGR"
     gene            complement(6332..7222)
                     /locus_tag="PKV56_11565"
     CDS             complement(6332..7222)
                     /locus_tag="PKV56_11565"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOX68495.1"
                     /translation="MLVSVAVTTASLLGESPFWHPREQALYYCDIPGHCLNRFDPVTG
                     ELSHWSFDTEVASCAPMLDGGLLLAARDGLWRFDAARGARTELAAPPYDPHTERFNDG
                     KCDPQGRFWVGTIYEPRDSARAALYRLHRGILTKRAGDITVSNGLAWSPDGRTMYWSD
                     TKAHAIYAFDFDLADGTLSGRRLFHQFPVKRDGQSLDDYGGRPDGAAVDVAGNYWVAM
                     FEGQRLLCLSPAGSVVREVSLPVRCPTMPCFGGADLRTIYITTAREKRPGEELAVQPL
                     AGCVLSMRVDLPGLPVNLFG"
     gene            complement(7252..8136)
                     /locus_tag="PKV56_11570"
     CDS             complement(7252..8136)
                     /locus_tag="PKV56_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HOX68496.1"
                     /translation="MSATTEPSPGHEARGALELRAGELRLAVRADLGGSIAGFWHRGK
                     PILRSTEPDLLAGSRQSACYPLVPYSNRLAYRRFRWKSRDYTTVANFDDNPHSVHGVG
                     WLRVWERLSHGDGEIVLRYRHTPDEHWPFAFDSQQSFALTPHSLVVQLSITNTAEIEQ
                     PVGLGWHPYFPKRERSELDIKLSDRWDSDASQLPVRKVAQAGMAGQVSELRFDHCFDG
                     WTGPACIRDEIFSLKLTSNLTHLVVYTPGDKPYFCVEPVSHVSDAIHMADSVAHGLIT
                     LQPGETLKASMALEVAVP"
     gene            complement(8214..8705)
                     /locus_tag="PKV56_11575"
     CDS             complement(8214..8705)
                     /locus_tag="PKV56_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX68497.1"
                     /translation="MPPSDIRLVVPSDDQLIDVTRGIFREYARSLGIDLCFQDFDAEL
                     AGLPGPYAPPHGHLLLALVDGELAGCGAFRRLVDVDYANACEMKRLYVRPAFRRFGLG
                     RLLAQALFDEARHAGYSTVLLDTLDGMEAARGLYSSLGFVEIPPYYYSPIAGSHYLKA
                     DLD"
     gene            complement(8826..9734)
                     /locus_tag="PKV56_11580"
     CDS             complement(8826..9734)
                     /locus_tag="PKV56_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX68498.1"
                     /translation="MKSATAAVEIRGLWLGLLGVAIFAMTLPMTRLAVGPASEPQLSP
                     WFVTAGRAALSGLLSAAYLLATHAPRPQRQQIGGLAVCALGTVVGFPLFLGLALRDVD
                     ATHAAVVSGVLPLVTAVIAALYLRQRPSMAFWACAVTGCALVMAFAAHHGSGRLRVAD
                     GLLLLAIASTSLAYVVGARLSTQMPAEQTICWVLVGSLPLTLPLMFALWPDRAVSWSA
                     WGGFAYVTLFSMWLGFFAWYRGLALGGTVRVSQVQLVQPFLALIFAVPVLGEQLEAST
                     VVFSLAMITVVLVSKRLSVGEAPALK"
     gene            9897..10658
                     /locus_tag="PKV56_11585"
     CDS             9897..10658
                     /locus_tag="PKV56_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68499.1"
                     /translation="MFDSTIPFGEPELMRASRFLIHLPSAGGPAASGIVSSGLASLSP
                     SLLADLQRCDGQEQRSEPLDVLAACVRHAQPVTVHLQYHDRVVPLTVFPHERLVHCPM
                     PINDLLTHRQPQLQVIHIEAALRAAPGAMATQRLLSGPGPVTDLHPLGAALWHLALHG
                     GRRELLPEIAGPAVYRVSFGLQMDDLSVTGALLAAIQRLRHEPCHLRDIADWPGFDRE
                     RAARMLNALYLQSGLIVSRSHPDALRESWFGALGR"
     gene            complement(10683..12587)
                     /locus_tag="PKV56_11590"
     CDS             complement(10683..12587)
                     /locus_tag="PKV56_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX68500.1"
                     /translation="MAVLSLSNAHLAFGHVALLDNAAFSLETGERLGLIGRNGAGKSS
                     MLKIIAGLEKLDDGLLQMTQGVRICYVPQEPVFEPGHTVFDAVSEGVSEARAVRQAYE
                     EHADGVDLDALQTRIEALDAWNWEQRVDTTLAQLHLDGTREIGQLSGGMKKRVALAQA
                     LVAVPDVLLLDEPTNHLDLDSIAWLEELLRGFKGSVMLITHDRAFLDGVATRIIELDR
                     GVIRSYPGNFSAYERMKEEQLASDALASARADKLLAQEEVWIRKGVEARRTRSVARIQ
                     RLEVLRDQRQKRRDSLGQVRLEVDSGAPSGKIVAELRDVSLSFGDKVIVKEFNATILR
                     GDKVGLIGPNGAGKTTLLKLILGELQPDSGKVRQGSKIEVAYFDQMRSTLNLDATLAD
                     TISPGSEWVEFAGQRKHVMSYLNDFLFSPERANSPVRTLSGGERNRILLARLFALPAN
                     VLVLDEPTNDLDIDTLELLEELLQAYKGTVFLVSHDRRFLDNVVTSTIVWEGDESPGQ
                     WREYEGGYEDWKIQRDRAQALRLQAARASKGKDTPPASKTAPVSATAVAPAAPKPRKL
                     SYKEQRELDELPGRIEALEAEQVTLATLLADPEAYVRDPKAVAAAQIRVAKIDEELLG
                     AMDRWELLQR"
     gene            12658..13527
                     /locus_tag="PKV56_11595"
     CDS             12658..13527
                     /locus_tag="PKV56_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX68501.1"
                     /translation="MDTDAIFHDSFYKFVDVADIDATAATLRDLTSALTGSLLVASEG
                     INGTLAGKPATLDAFRVALATDPRLEGRFTGIAFKRSPCVTPPFQRMKVHGRREILPL
                     GVEGVNAVGHIGHQVTPQQWRDLLDDPNVVVIDNRNHFEFRLGRFRNAIDPGVRNFRD
                     FPAYMEAQLPTWKAEGKRVAMYCTGGIRCEKTSAWLEERGVPVLQLEGGILNFFADMP
                     DAERDWEGECFVFDNRVALDTHLRETGTTAEQVYAEDPDEAWRLRRARQLASAGQSDA
                     LAGPTGTAAKPAT"
     gene            13524..14471
                     /locus_tag="PKV56_11600"
     CDS             13524..14471
                     /locus_tag="PKV56_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX68502.1"
                     /translation="MTAPAPLPTKDGVGPSCVALPPGPWATIGDFLVDRFPAVSAETW
                     RSRMQQGEFVDEHGVAVEPDRPYRASLRLYYYRSIADEPRVPFDEIVLFQDDLLVVAD
                     KPHFLPVTPGGRHLQETLLVRLKRRLGIDTLAPVHRLDRETAGVVVFTVKPHTRGAYQ
                     SLFAQREVMKHYEAIAPWRPERSFPLTHCSRLAQDDHFLRMREVPGEPNSETLFEVIE
                     AGDEWARYRLTPLTGRTHQLRVHCAAIGMPIAGDRMYPTLQAADTDDHERPLQLLARA
                     IGFVDPISGEARQFDSARELNSIQDVERLTPRGVPPAHR"
     gene            complement(14437..15396)
                     /gene="gshB"
                     /locus_tag="PKV56_11605"
     CDS             complement(14437..15396)
                     /gene="gshB"
                     /locus_tag="PKV56_11605"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOX68503.1"
                     /translation="MKLLFVADALEGFNTAKDTTFSMMREAAARGHALLACQPKDLMW
                     QRGGRVTGFVREITLTPDGPAWFSARQQPPDEIPIALADVDAVVMRKDPPFDSEYFYA
                     THLLGQAEREGARVFNRPAALRDHPEKLAILEFPQFIGPTLVTRDAGDIRRFHAEHRD
                     LILKPLDGMGGMGIFRVGPDALNLGSIIETLNRHGAQTVMVQKYLPEITQGDKRILLI
                     DGVPVPHALARIPQGSEIRGNMAVGGKGVAQPLGPRDREIAEAIGPVLAARGLLLVGL
                     DVIGDCVTEINVTSPTGFQEITKQTGFDVAKVFIDALEARLAA"
     gene            complement(15519..>16462)
                     /locus_tag="PKV56_11610"
     CDS             complement(15519..>16462)
                     /locus_tag="PKV56_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOX68504.1"
                     /translation="SVTKQAIQLGYLPRLRISHTSVKETGQVYVPFVNWSLYACIVLA
                     VIVFGSSSKLAGAYGIAVTIDMLITTTMTFFVIRYGWKYPWSLCLAATGFFFVVDTVF
                     FAANAVKLFDGGWFPVVLGALMFTLMMTWKQGRRLMRDRMRDDSIDLKSFLDAVFVSP
                     PTRVPGTAVFLVSEQGFTPNALLHNLKHNKVLHETNLFVTVEHLEIPWLSFDKRCRIE
                     SLGRDCWQVTMSFGFKNDPDVPESLELLKLRGVELDPMDTSYFLSRDIVIPTLGHGMA
                     MWREKLFASMHRNAAAAADFLRLPANRVVELGSKVEI"
BASE COUNT         3134 a         5357 c         5098 g         2873 t
ORIGIN      
        1 cggccagcgt gtagcggtag atgttgtcga cgaagaacag cacgtccttg ccttcatcgc
       61 ggaacgactc ggcaatggtc aggccggtca gcgccacacg cagacggttg ccggggggct
      121 cgttcatctg accgaacacc atcgcgacct tgtcgagaac gttggagtcc ttcatctcgt
      181 ggtagaagtc gttgccctca cgggtacgct cacccacgcc ggcgaacacc gacagaccgg
      241 agtgctgctt cgcgatgttg ttgatcagct ccatcatgtt cacggtcttg ccgacgccgg
      301 cgccaccgaa caggccaacc ttgccgccct ttgcgaacgg gcagaccaag tcgatcacct
      361 tgatgccggt ctcgagcaag tcctgcgacg ggctgagctc atcgtaagcc ggcggcttgc
      421 ggtggatcga ggcggtgagt tcctgatcga cagggccgcg ctcgtcgatt ggtctgccca
      481 gcacgtccat gatccggccc agggtcgcgc gacccactgg cacggtgatg gggttctggg
      541 tgttcgtgac cgtcaggccg cgacgcagtc catcagagga acccagcgca atggtacgca
      601 ccacaccgtc gcccaactgc tgctggactt cgagggtcag gcccaggctg tcgagcttca
      661 gtgcgtcgta aatcttcggc atcgcgttgc ggggaaattc cacgtcgacc accgcgccga
      721 tgcactggac gatcttgcct tctgtagcca ctgcggacat gttcgtatcc ttagaaaaaa
      781 cgaattctgg agttctgggg tcgtggatgg tgccgctcag acggcagccg cacctgcgac
      841 gatttcagac agttctttgg tgatcgccgc ctgacgcgtc ttgttgtaga ccagcttgag
      901 ttcagcgatc acggagcccg cgttgtcggt ggcggacttc attgccacca tgcgcgcgga
      961 ctgctctgac gccatgttct cggcaaccgc ctggtagacc agcgcctcgg tgtagcgcag
     1021 caacaactcg tcgatcaccg tctcggcatc gggctcgtac aggtagtccc agccgtactt
     1081 gccagcgcct gctggtgtgt cgacttccat gctcttcgaa gacagcggca acagctgctg
     1141 caccagcggc tcctgcttca tcgtgttgat gaacccggtg tagcaaagat agacggcgcc
     1201 cagcttccct tcggcatacg catcgagcag aaccttgacc gggccgatca acttctcgag
     1261 atgcggcttg tctccaagct gggtggcgtg cgagaccact tttgcaccga tgcggttcag
     1321 gaatccgagc cccttgttgc cgatcgccac ggcttcagcc ttgagtccct ggccttcgat
     1381 ttccttcagc ttcagggtga ccgcacgcaa cacgttggtg ttcatgccgc cgcacagccc
     1441 cttgtcagtc gtgacgacga tgaatccgac cgacttcact cccgtgttct ggaccaggaa
     1501 cgggtgcttg tactcgggcg tggctttcga gaggttggcc gcgagactgc gaatcttctc
     1561 ggcgtatggc cgggccgcac gcatgcgatc ctgggccttg cgcattttcg aagcggcgac
     1621 catttccatg gccttggtga tcttcttggt gttctcgaca ctcttgatct tgccgcgtat
     1681 ttccttgcct gctgccatga cgaagctcct gggtgattcg agagcgcgtt aggcgaagga
     1741 cttcttgaat gcggccacgg cagacgacaa ctcggcttcc gcgtccttgt cgagtgcctt
     1801 ggcgtcttcg atcttcttca gcagaccagc gtggctggtc ttcaggaact ggtgcaggcc
     1861 cgattcgaag gccagcacct tcttcacgtc cacatcgtcc atgaagccct tgttgaccgc
     1921 aaacagagtg gcacccatga gggagatcgg cagtggcgag tactgggcct gcttcagcaa
     1981 ctcggtcacg cgcgcacccc gatcgagctg cttgcgggtg gcttcgtcga ggtcggacgc
     2041 gaactgcgcg aaggctgcga gttcacggta ctgggccagg tcggtacgga taccgcccga
     2101 caggctcttg atgaccttcg tctgcgctgc gccaccgact cgcgacaccg aaataccggc
     2161 gttgatggcg ggacggatac cagcgttgaa caacgaggtt tccaggaaga tctgaccatc
     2221 ggtgatcgag atcacgttgg tcggcacgaa cgcggacacg tcaccggcct gcgtctcgat
     2281 gatcggcaga gcggtcagcg aaccggtctt gcccttgacg gcacccttgg tgaaagcctc
     2341 tacgtagtcg gcattcactc gtgcagcgcg ttcgagcaga cggctgtgga gatagaacac
     2401 gtcgccggga tacgcttcgc ggcctggcgg acgacgcagg agcagggaaa cctggcggta
     2461 ggcaactgcc tgcttcgaca gatcgtcata gacgatcagc gcgtcttcgc cacggtcgcg
     2521 gaagtactca cccatcgtgc agcccgaata ggccgacacg tactgcatcg cggccgattc
     2581 cgcagcggtc gcagcaacga cgatggtgta ttccatcgcg ccattggcct cgagcgcgcg
     2641 cacgacgttc ttcaccgacg aggccttctg gccgatcgcg acgtagatgc acgtcatgtt
     2701 ctgaccgcgc tggttgatga tcgcgtcgat tgccacggca gtcttgccgg tctggcggtc
     2761 accgatgatc agttcgcgct gaccgcgacc gactggcacc atcgagtcaa tggacttcag
     2821 acccgtctgc attggctggt ccaccgactt gcgagcaatc acgccagggg cgatcttctc
     2881 gatcggcatg gagagctgcg cgttgatcgg acccttgcca tcgatgggct ggcccagcgc
     2941 attgaccacg cggcccttga gctcggggcc gaccggcact tcgagaatgc ggccggtgca
     3001 cttgacagtg tcgccttcgg caatgtgttc gtactcaccc aagatcaccg cacccaccga
     3061 atcacgctcc aggttcagcg cgagaccgaa ggtcgatgaa ccgtccggcg aggccgggaa
     3121 ttcgagcatt tcgccctgca tcacatcaga caagccgtgc acccggcaaa tgccgtctgc
     3181 cacggacacc acggtgccct gattgcggat gtcggtggaa gcaccaaggc cctcgatgcg
     3241 gctcttgatg agttcggaaa tttcagcggg attcagttgc atgagctctc tcctgtctgt
     3301 gcgttcgccc ctgcaggtgc ggcgctcgtc agagtgaatg actgggaatg tgaccggacg
     3361 gttttcgcgg cgatcaagcc gtgagtgcca cccgcatttg ttcaagacgc gccttcaccg
     3421 aggtgtccat gacctcatcg ccgacgacaa cacggatgcc cccgatcagc gctggctcga
     3481 gctgcaccac cacgttcagc ttgcgtgcga agcgcttttc cagcgttgcg accacatcgg
     3541 ccagctgcgt cgcctcgatg ggaaacgcac tgaaaaccgt ggcgtccgag actcccgctc
     3601 ctgcgttggc gagagcgtga aactgctgag cgatttcagg catcacggtg agacgaccgt
     3661 tgtcgatgac cgtgcgaagc aggttcttca tcggctcgga cagcggcgac ttcacgaccg
     3721 acgcgatcag atcgaacacc tgttcggagc tgaccttggg gctgttcgcg aattcacgca
     3781 gctgcgcgtt gccggctatt gccgccagcg catcgacctg ctgcgcaaac gcagccacgt
     3841 caccctgcga agcgacgtcg aacaaggctt cggcataggg ccgggccaga gtagcaagtt
     3901 cggccatggt tcagcccccc gacgagcagc cgccgccgct cacagctcga ccttcaggcg
     3961 gttcaacaag tcggcgtgaa cgccggcatt gacttcgcgc tggagaatct gttcggcccc
     4021 cttgacggcg agcgctgcca cctgctcgcg caggatctcg cgcgccttga tcgcctcttg
     4081 ctcggcctcg gccttcgcag cggaaacaat cttcgcgcct tcttcgttgg cacgcgtctt
     4141 cgcctcttcc accatcgatt gcgccagacg ctcggcatct gccagacgtt gcgccgcatc
     4201 ggtacgcgcc gcagaaagct gctcctcgac gcgcttgttg gcggtggtca gctcagcctt
     4261 tgccttgtcg gcagccgaca ggccatcggc gattttctga gcgcgcgcat cgagtgcagc
     4321 ggtgatcggt ggccagatgt acttcatcgt gaatccgacc aggatcagaa acacgatcat
     4381 ctgaatgatg aatgtcgcgg tgatgctcac gttgaggcct caatcagggt agcggggtat
     4441 cggtcaacgg gcccgaatga atcgggccga tggccttgcg cgatttgcgc gacttacttc
     4501 ggcaggttcg cgatttgagc caggaacggg ttggccgtcg tgaaccacag cgcgataccg
     4561 gtaccgatga tgaacgatgc gtcaatcaga ccgaccagca ggaacatctt ggtttgcagc
     4621 tcacccatca gttcgggttg gcgtgcagct gactcgaggt acttgctgcc catgacgccg
     4681 atgccgatac aagcacccat ggcgcccaga ccgatgatga gacctgcggc aaccgcgacg
     4741 agaccaatga gttccatgat gattcctgtg atgaaaaaag agaacgagtg agagagaaaa
     4801 cgagatcagt gcgtctcgtg ggcctgcccg atgtagacca gcgtcaacat catgaacacg
     4861 aaggcttgca acgtgatgat caggatgtgg aagatggccc acacggtccc cgcgaggatc
     4921 tgcagggccg ccagggcaaa accgccgagc gacaaggtaa acgcaccgcc caacaacgcg
     4981 atgagcagaa agatcaactc acccgcatac atgttgccga acaaccgcat tccgtgcgag
     5041 acggtcttgg caacgaactc gacgatctgc atcgcgaaat taaagggata gagagcccag
     5101 tggtttccga atggcgcggc aaacaactca tgcacccaac cgccgaaacc cttgatcttg
     5161 acgttgtaca caagacaggt gatcaggacg ccgaccgaaa gacccatcgt gatggaaagg
     5221 tcggcggtcg gaaccacccg caggaacgcg tgatgaggat cgccgcccgc cgcaacgtag
     5281 atcttttccc agatgagcgg cagcagatcg accggcaaca ggtccatcgc gttgagcagg
     5341 aaaatccaca cgaagaccgt cagagccagc ggcgcaacca gcttgcgact ttcggcgttg
     5401 tgcacgatcc ccttggcctg gctttcgacc atctcgaaca agatctcgac agcagcctgg
     5461 aaccgcccag gcacgcccgc cgtcgccttg ctcgcagccc tccacagcag gaaacatccc
     5521 agcacaccca ggagcgtcga gataacgatg gaatcgaaat tgatgacgga aaagtcaacc
     5581 agactggcag gtttgccggt cgacaaatga gtcaggtggt gaacgatgta ttcaccggaa
     5641 gtgagcgcgt gaccttcggc tgacatgtga atgaatcctg tctgatctag ttctcaacgc
     5701 ccgcgccaaa gcagagcgac caaatacacc tgcatgcaca gcaccagcgt cgccagcaag
     5761 accggccaac tcagggactg aaccagcttc ggcgccagca tgagcatggc aatcgaaact
     5821 ccgattttca tcacggtcca caacatgaca ctgacggcac tgctgcctac agacagactg
     5881 gaaagcctgc tggtcatgcc acgcgccatc agggcgcccg gaagcaccgc aacgatggag
     5941 ccatagatag ccgactgcac aaacccgggc tggtcggaaa accaccacgc gcccagagcc
     6001 atcaaacaac ccaccacggc ctgtgccgcg ataacccgcc agggagacag ttgcgggctt
     6061 ttgacccgca aggcctgagc ctcgtctcgc gtcaacggct tgacgggcgg ctcttcagcc
     6121 tcatcgtcaa aaccagcgct tgccacacca cgggcgatca gttgtgtcat cggacgggta
     6181 acaaacgctt tcagtacagc ctcgaattat acgtgcaaac ccgcagcctc ctcaaccctc
     6241 ggtttgacag gtggcaccac accccgcgcc gggggctcaa cggaaggctc gctcatgacg
     6301 acgagccagg tgccccgctc catgagcgga ctcaaccgaa caggttgact ggcaggccgg
     6361 gcaggtccac ccgcatcgat aacacgcagc ctgccaatgg ctgcacagcc aactcttcgc
     6421 ctgggcgctt ctcgcgtgcc gtcgtgatgt agatcgtcct cagatcggcg ccgccgaagc
     6481 aaggcatcgt ggggcatctg acaggcagtg acacttctcg cacgacgcta cctgcaggag
     6541 acaggcacag cagccgctgc ccctcgaaca tggcgaccca gtagttgcct gccacatcga
     6601 ccgcggcacc atcgggacgg ccgccgtaat catcgagcga ctgcccatcg cgcttcaccg
     6661 ggaactgatg gaacaggcga cggccgctga gcgtgccgtc ggcaagatcg aaatcgaagg
     6721 cgtagatggc gtgcgccttg gtatcgctcc agtacatcgt gcggccatcc ggactccacg
     6781 caagcccgtt ggagacggtg atgtctccgg cccgcttggt caggatgccg cgatggagcc
     6841 tgtaaagcgc agcccgggcg gaatcgcggg gctcgtagat cgtgccgacc cagaaacgcc
     6901 cttgcgggtc gcactttcca tcgttgaatc gctcggtgtg cgggtcgtag ggcggcgcgg
     6961 ccagctcggt gcgggcgcct ctggccgcat caaaacgcca gagtccgtcc cgcgccgcca
     7021 gcagaagacc accatcgagc atcggtgcac agctcgccac ctcggtgtcg aagctccagt
     7081 gcgacagctc gcccgtgacc gggtcgaacc gattcaggca gtgccccggt atgtcgcagt
     7141 aatacagcgc ctgctcgcgc gggtgccaga acggcgattc accgagcaga ctggccgtcg
     7201 tcacggcgac cgaaaccagc acatcggatg cgtgaggcac ggctcccgta ctcacggaac
     7261 cgccacttcc agcgccatcg aggccttcaa cgtctcgcca ggctgcaacg tgatcagccc
     7321 atgggccacc gaatcggcca tgtggatcgc gtcactgacg tgactgaccg gctcgacaca
     7381 gaagtagggc ttgtcgcctg gcgtgtagac caccagatgg gtcaggttgg aggtgagctt
     7441 cagcgagaag atttcgtcgc ggatgcatgc cggaccggtc cagccgtcaa agcaatgatc
     7501 gaaacgaagt tccgacacct gtccggccat gccggcctgc gccaccttgc gaaccggaag
     7561 ctgggaagca tccgaatccc accggtcact cagcttgatg tccagctcac tgcgctcacg
     7621 cttcgggaaa tacggatgcc agcccaagcc gaccggctgc tcgatttccg ccgtgttggt
     7681 gatgctcagc tgcacgacaa gcgaatgggg agtcagcgcg aaagactgct gcgaatcgaa
     7741 cgcgaatggc cagtgctcat ccggcgtgtg ccgatagcgc agcacgattt cgccgtcgcc
     7801 gtgcgatagc cgctcccaga ccctcagcca gccgacaccg tggaccgaat gcgggttgtc
     7861 atcgaagttg gcgaccgtcg tgtaatcccg actcttccaa cggaagcgcc tgtaggccag
     7921 ccggttcgaa tacggcacca gcggatagca ggcagactgc cgcgaacctg ccagcaagtc
     7981 gggttcggtc gatcggagga tcggcttgcc gcgatgccaa aacccggcga tcgaacctcc
     8041 gagatcggca cgcaccgcga ggcgcaattc gcccgcgcgc agttcaagcg cacctcttgc
     8101 ctcgtgccca ggagaaggtt cggtagtggc actcatgaat cttcagtggc tgtgagttga
     8161 actgccccat tgggagccgg ctggaccaga agccatccta cgccgccacc gcatcagtcc
     8221 agatcggcct tcaggtaatg ggacccagcg atcgggctgt agtagtaggg gggaatctcg
     8281 acgaagccca gcgagctgta gagcccgcgt gctgcttcca tgccgtccag cgtgtcgagc
     8341 agcaccgtcg aatacccggc atggcgcgcc tcgtcgaaca gtgcctgagc cagcagtcga
     8401 cccaggccga agcgtcgaaa tgccgggcgc acataaagac gcttcatctc gcaggcattc
     8461 gcgtaatcga catcaacaag ccgcctgaac gcgccgcaac cggccagctc tccgtccacg
     8521 agggcaagca gcagatggcc gtgaggtggt gcatatggtc cgggcaagcc ggccagctcg
     8581 gcgtcgaaat cctggaagca caggtcgatg cccagactgc gcgcgtactc acgaaagatc
     8641 ccccgggtga cgtcgatcag ttgatcgtcg ctcgggacca cgaggcgtat atcggagggc
     8701 ggcatggggt tcgggcggct ggacaacgcg gtttcagttt atcgccggcc agcggaccgc
     8761 ctttcggtgg tcgccgcgcg cattcttcat gccgcatgct aaaaaaacac gtccatcgga
     8821 acacatcatt tgagggcagg cgcctcccca accgacaggc gcttgctcac cagcacgacc
     8881 gtgatcatcg caagcgagaa cacaacggtg ctcgcctcca gttgctcgcc cagcaccggc
     8941 actgcgaaga tcagcgccag aaacggctgc accaactgca cctgactgac acgcaccgtg
     9001 ccgcccagcg ccagacctcg gtaccacgcg aagaagccca gccacatcga gaacagggtg
     9061 acgtaggcaa agccgcccca ggcactccag ctcaccgctc ggtcgggcca caacgcgaac
     9121 atcagcggca atgtgagcgg caagctgccg accagcaccc agcagatggt ctgctcggcc
     9181 ggcatctggg tcgacagccg ggcgccgacc acataggcca gggacgtgct ggcaatggcc
     9241 agcaacagca agccgtcggc gacacgcagg cgcccggatc catggtgagc ggcaaaggcc
     9301 atcaccagtg cgcagccggt gacggcgcag gcccagaacg ccatcgacgg gcgctgccgc
     9361 aggtacaacg ctgcgatcac cgcagtgacc agcggcagca caccgctgac aacggcggcg
     9421 tgcgtggcat cgacatcgcg cagcgccaga ccgagaaaca gcgggaagcc aaccaccgtc
     9481 cccagggcgc acaccgcgag gcctccgatc tgctggcgct ggggacgcgg ggcatgggtc
     9541 gccagcagat aggcggcact cagcaagccg gacagcgcag cgcggccggc agtcacgaac
     9601 cacggcgaca gctgcggctc gcttgcaggc ccgaccgcca ggcgggtcat cggcaaggtc
     9661 atcgcgaaga tcgccacgcc gagcagaccc agccagaggc cccggatctc gacagcggca
     9721 gtggcggatt tcatcaatgg gcgtgaaagc tgcggagggg ctgcagtcta cgaacaccgc
     9781 gcgtctgccc aagccacccc cggatgcaat ctgtacttga gcgaacggtc tatcgaacct
     9841 gatacaaccg caaacagaag acgctgttgg ctacaacctg atcccgcgcg accgcgatgt
     9901 tcgattccac catccctttt ggcgaacccg aactgatgcg ggccagccgg ttcctgatcc
     9961 accttccgag cgcaggcggc ccggccgcgt ccggcatcgt gagctccggc ctcgcttcgc
    10021 tgagcccttc gcttctggcc gacctgcaac gctgcgatgg tcaggagcag cgctccgagc
    10081 cgctcgacgt gctggccgcc tgtgttcggc atgcccagcc ggtcacggtc cacctgcagt
    10141 atcacgatcg cgtggttccg ctgaccgtgt tcccgcacga gcggctggtt cactgcccga
    10201 tgcccatcaa tgacttgctg actcatcggc aaccgcagtt gcaggtgatc cacatcgagg
    10261 cggcactgag ggcagcccca ggggccatgg cgactcagcg gctgctatcg ggcccagggc
    10321 cggtcacgga tctgcatccg ctcggtgccg ctctctggca cctggcactc catggcggac
    10381 gccgcgaact gctgccggaa atcgccgggc ccgcggtgta ccgggtcagc tttggcctgc
    10441 agatggatga cctgtcggtc actggcgcac tgctggcagc cattcagcgc ctgcgtcacg
    10501 agccctgcca ccttcgcgac atcgccgact ggccgggttt cgatcgcgaa cgcgccgcgc
    10561 gcatgctgaa tgcgctttac ctgcagtcag gattgatcgt cagccgctcc catccggatg
    10621 cattgcggga gagctggttc ggtgcgctgg gtcgctgaag cagcgaagta caacgccatg
    10681 cctcagcgct ggagcagttc ccagcggtcc atggcgccca gcaattcttc atcgatcttc
    10741 gccaccctga tctgcgctgc cgcaacagcc ttcgggtcgc ggacgtaggc ctcgggatcc
    10801 gccagaaggg tcgcgagggt gacctgttcc gcctcgaggg cctcgatgcg gcccggcagt
    10861 tcgtcgagct cgcgctgctc cttgtagctg agcttgcggg gcttcggagc cgcgggggcg
    10921 acagcggtcg ctgacaccgg cgcagtcttg ctcgcgggtg gtgtgtcctt tcccttggaa
    10981 gcccgggctg cctgcaggcg caacgcctgc gcccggtcgc gctggatctt ccagtcctcg
    11041 tagccgcctt cgtattcgcg ccactggccg ggcgattcgt caccctccca gacgatggtg
    11101 ctggtcacga cgttgtcgag gaagcgcctg tcgtgactga ccaggaatac cgtgcctttg
    11161 taagcctgca acagctcctc gagcagttcc agcgtgtcga tgtcgaggtc gttggtcggc
    11221 tcgtcgagca ccagcacgtt ggcgggcaag gcaaacagtc gggccagcag gatgcgattg
    11281 cgctcgccac cgctgagcgt gcgcaccggc gagttggcac gctcgggcga gaacaggaaa
    11341 tcgttcaggt agctcatcac gtgcttgcgc tgaccggcga actcgaccca ttcgctgccc
    11401 gggctgatcg tgtcggcgag ggtcgcatcg agattcagcg tgctgcgcat ctggtcgaag
    11461 tacgcgacct cgatcttgct gccctggcgc accttgccac tgtcgggttg aagttcgccg
    11521 aggatcagct tgagcaacgt cgtcttgccg gcgccattgg gaccgatcag tccgaccttg
    11581 tcgccgcgca ggatggtggc attgaattcc ttgacgatga ccttgtcgcc gaaactcagc
    11641 gacacgtctc tcaattcggc gacgatcttg ccactcggcg cacccgagtc gacttccagt
    11701 ctcacctgac cgagcgagtc gcgacgcttc tgacgttgat cgcgcaggac ctcgaggcgc
    11761 tggatgcgcg cgacgctgcg ggtgcgccgt gcctcgacgc ccttgcgtat ccacacctcc
    11821 tcctgggcca gcagcttgtc cgcacgggcg ctggccagtg catcggacgc aagctgctct
    11881 tccttcatcc gctcgtaggc gctgaagttg ccggggtagc tgcggatgac acctcggtcg
    11941 agctcgatga tgcgcgtggc cacgccgtcg aggaatgcgc ggtcgtgggt gatcagcatg
    12001 acgctgccct tgaagccgcg cagcagttcc tcgagccagg caatcgagtc gaggtcgagg
    12061 tggttggtcg gctcgtcgag cagcagcaca tcgggcaccg caaccagcgc ctgcgccagc
    12121 gcgacgcgct tcttcatgcc acccgacaac tggccgatct cgcgcgtgcc gtcgaggtgc
    12181 aactgcgcca gcgtcgtgtc gacccgctgc tcccagttcc acgcatcgag cgcctcgatg
    12241 cgggtctgca gcgcatcgag atcgacgcca tcggcgtgct cctcgtaggc ctgacgcacc
    12301 gcgcgtgcct cggacacgcc ttcgctgacc gcatcgaaca cggtgtggcc gggctcgaac
    12361 accggctcct gcggcacgta gcagatgcgc acgccctgcg tcatctgcag caggccgtcg
    12421 tcgagcttct cgaggccggc gatgattttc agcatcgacg acttgccggc gccgttgcgg
    12481 ccgatcaggc cgagccgttc gccggtttcg agcgagaacg ccgcgttatc gagcagggcc
    12541 acgtggccga aggccaggtg ggcattggag agggagagga cagccatgcc tcgattgtga
    12601 gggtcgaccc tgatacggtc ctgctaccag cgccacggca aggtcgaaaa tgccgtcatg
    12661 gacaccgatg cgatctttca cgactccttc tacaaattcg tcgatgtcgc cgatattgac
    12721 gccaccgctg cgacgctgcg cgacctgaca tccgcgctga cgggcagcct gctggtcgcc
    12781 agcgagggca tcaacggcac gctggccggc aagcctgcga cgctcgatgc gttccgcgtc
    12841 gccctggcaa ctgacccgcg gctcgaaggc cgcttcaccg gcatcgcgtt caagcgcagc
    12901 ccctgcgtca cgcccccgtt ccagcggatg aaggtgcacg gtcggcggga aatcctgccg
    12961 ctgggcgtcg agggcgtcaa tgccgtcggc cacatcggcc atcaggtgac gccgcagcaa
    13021 tggcgcgacc tgctcgacga cccgaacgtc gtcgtgatcg acaaccgcaa tcacttcgag
    13081 ttccggctcg ggcgcttccg caacgccatc gacccgggcg tgcgcaactt ccgtgatttc
    13141 ccggcctaca tggaggcgca gctgccgacc tggaaggcgg aaggcaagcg ggtcgcgatg
    13201 tactgcaccg gcggaatccg ctgcgagaag accagcgcct ggctcgagga acgcggcgtg
    13261 cccgtgctgc aactcgaagg cggaatcctc aacttcttcg ccgacatgcc tgacgccgaa
    13321 cgtgactggg aaggagagtg cttcgtcttc gacaaccgcg tcgcgctcga cacccacctg
    13381 cgtgaaaccg gcacgacggc cgagcaggtc tatgcagagg accccgacga agcgtggcgg
    13441 ctgaggcggg cgcggcaact ggcaagcgca gggcaatccg atgcgctcgc cggaccgact
    13501 ggcacggccg ccaagccggc aacgtgaccg ccccggcgcc gctgccgaca aaagacggcg
    13561 tcggtccgag ttgcgtcgcg ctgccgccgg ggccatgggc gacgattggc gacttcctgg
    13621 tcgatcggtt tccggcggtc tcggccgaga cgtggcggtc acggatgcag caaggcgagt
    13681 tcgtcgacga gcacggcgtc gctgtcgagc cggacaggcc ttaccgggcc tcgctgcggc
    13741 tgtactacta ccgctcgatc gctgacgagc cgcgcgtgcc gttcgacgag atcgtgctgt
    13801 tccaggatga tctgctcgtg gtcgccgaca agccgcattt cctgccggtc acgcccggcg
    13861 gccgccacct gcaggagaca ctgctggtgc ggctgaagcg ccgtctcggc atcgacaccc
    13921 tggcgccggt ccaccggctc gaccgcgaaa ccgctggcgt cgttgtcttc acggtgaagc
    13981 cgcacacgcg cggggcctac cagtcgttgt tcgcacaacg cgaggtcatg aagcactacg
    14041 aggcgatcgc gccgtggcgc ccggaacgct cgtttccatt gacgcactgc agtcggctgg
    14101 cgcaggacga ccacttcctg cgcatgcgcg aggtgcccgg cgagccgaat tccgagacgc
    14161 ttttcgaagt gatcgaggca ggcgacgaat gggcgcgcta ccgactgacc ccgctgaccg
    14221 gacgcaccca ccagctgcgc gtgcactgcg ccgcgatcgg catgccgatc gccggagacc
    14281 gcatgtatcc gacgctgcag gccgccgaca ccgacgacca cgaacgacct cttcagttgc
    14341 tggcgcgcgc gatcggcttt gtcgacccga tcagcggcga agcgcggcag ttcgacagcg
    14401 cacgcgagct gaattcgatt caggacgttg agcggctcac gccgcgaggc gtgcctccag
    14461 cgcatcgatg aacaccttag ccacatcgaa ccctgtctgc ttcgtgatct cctgaaaccc
    14521 ggtcggactg gtgacgttga tctcggtcac gcagtcgccg atgacgtcca ggccgaccag
    14581 cagcaagccg cgagcggcga gcaccgggcc gatggcttcg gcgatctcgc ggtcgcgcgg
    14641 gccgagcggt tgcgccacac ccttgccgcc caccgccatg ttgccgcgga tttcgctgcc
    14701 ttgcggtatg cgcgccaggg catgcggcac cggcacgccg tcgatcagca ggatgcgctt
    14761 gtctccctgc gtgatctccg gcaggtactt ctgcaccatc accgtctgcg cgccatgccg
    14821 gttcagcgtc tcgatgatgc tgccgaggtt caacgcatcg gggccgacac gaaagatccc
    14881 catgccgccc atgccatcca gcggcttcag gatcagatca cggtgctcgg catggaagcg
    14941 ccggatgtca cccgcgtcgc gcgtgacgag cgtggggccg atgaactgcg gaaactcgag
    15001 gatcgcgagc ttctccgggt ggtcgcgcag cgccgcagga cggttgaaca cgcgtgcgcc
    15061 ctcgcgctcg gcctgaccga gcagatgggt ggcatagaag tactcgctgt cgaagggcgg
    15121 gtccttgcgc atgaccactg catccacgtc ggccagcgcg atgggaatct cgtcaggcgg
    15181 ctgctggcgg gcggagaacc acgcggggcc gtccggtgtc agcgtgatct cgcgcacgaa
    15241 gccggtgacc cggccgccgc gctgccacat caggtccttg ggctgacagg cgagcagcgc
    15301 atgtccgcgg gcagcggcct cgcgcatcat cgagaaagtg gtgtccttcg cggtgttgaa
    15361 accttcgagc gcgtcggcga cgaacagcag cttcatggag tggtctttct cggaaggcct
    15421 ggaaatgtcc gtcgccactg ttgcacaacc ggcgccagag cgcccggagc catgcggaca
    15481 cggggctcga ggcagccctc agggggccag acgcacgatc agatctcgac cttcgagccc
    15541 agctcgacca cgcggttggc gggcaacctg aggaagtcgg ccgccgctgc tgcgttgcgg
    15601 tgcatgctcg caaacagctt ctcgcgccac atcgccatgc cgtggccgag cgtgggaatc
    15661 acgatgtcgc gagacaggaa atagctggtg tccatcgggt cgagctcgac gccgcgcagc
    15721 ttgagcaact cgagcgactc cggcacatcc ggatcgttct tgaagccgaa gctcatcgtc
    15781 acctgccagc agtcgcgacc gagcgactcg atcctgcaac gcttgtcgaa gctcagccac
    15841 gggatctcga ggtgctcgac ggtgacgaac agattggtct cgtgcagcac cttgttgtgc
    15901 ttcaggttgt gcagcagcgc gttcggcgtg aacccctgct cgctgaccag gaacactgcc
    15961 gtgccgggca cccgcgtcgg cggactcacg aacaccgcgt cgagaaagct cttcagatcg
    16021 atcgagtcgt cgcgcatgcg atcgcgcatc agccgacggc cctgcttcca ggtcatcatc
    16081 agcgtgaaca tcagcgctcc gagcaccacc gggaaccagc cgccgtcgaa cagcttgacc
    16141 gcgttggccg cgaagaagac cgtgtcgacg acaaagaaga agccggtcgc ggccaggcac
    16201 aacgaccaag ggtacttcca gccgtagcgg atgacgaaga aggtcatcgt cgtcgtgatc
    16261 agcatgtcga tcgtgaccgc gatgccgtag gcaccggcca gcttgctgct agagccgaag
    16321 acgatcaccg ccagcacgat gcaggcgtag agactccagt tgacgaatgg cacgtagacc
    16381 tgacccgtct ccttgacgga ggtgtgcgag atacgaaggc gaggcaggta gccgagctgg
    16441 atggcctgct tggtcaccga ga
//
LOCUS       DAOORV010000081        16319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4333_length_16319_cov_7.098992, whole genome shotgun sequence.
ACCESSION   DAOORV010000081 DAOORV010000000
VERSION     DAOORV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4333_length_16319_cov_7.098992"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKV56_11615"
     CDS             <1..252
                     /locus_tag="PKV56_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68505.1"
                     /translation="RAIVKRPEVLLCDEPTGALDYATGKLVLEVIERINRETGTTAVV
                     ITHNAAIAGMADRVIHLGDGRIQRVETNAHKLSPSQLSW"
     gene            255..2627
                     /locus_tag="PKV56_11620"
     CDS             255..2627
                     /locus_tag="PKV56_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68506.1"
                     /translation="MKALDRKLMRDLRAMWSQVLTIALVVASGVAGFITTLSAVDSLA
                     LARDRFYAEARFADVFAGLKRAPLTVADTLRALPGVADVQTSVEVAVRVEIEGRSEPI
                     VGQLIGVDPRRPARMNLVVLRGAAAAPSPGPPLADGSIPAWVSESFATANQLKPGSRL
                     SALINGKRRTLLMTGVALSPEYVFAGLWGMPDQRGFGVFWVDHEALAAAFDMAGAFNH
                     LALKLAPHGDERTVIDGLTGVLDTYGGRQAHGRTDQTSHAMLDNEIKEQRVLGTLLPS
                     IFLGVAAFLLNVVIARLVSTQREQIAALKALGYPNATIALHYLKMVLVIVSIGWLLGL
                     VIGDQLGAALTRLYSDFFRFPSFEHRVAPWLLLVSGGLTLATAVLGTLNAIAATARLA
                     PAEAMRPQAPGRYRRTLFERMGIRHIPTSLRMILRQMERRPGRTALAVGGVAASVAIV
                     VMGNFLRDAIESIVDTQFTLAMRNDVAVWTLDPLIDASGRELARLPGVTAVESNRYVP
                     VTLVNGHRRERSQLRGYALQPELYRIVDVNRRQTQPTGDGLVLTDRLADKLGVRVGDR
                     VQVEVLEGRARTLDLVVDATVREMMGLNAYMNRPALNRALGDGDLSTGFMLAVEPGRE
                     AELLDATRQLPRVAGAFSKATMLRNMQELSARNVRIMSTVLTVFASIIAVGVVYNNAR
                     IALAERTWELASLRVLGFTRAEVSGLLLGEMVIVVALALPLGMLLGWGLVHVLAGALK
                     SDQFYFPVVILPRTYAWAAICVVGAALGSALVVRRRIDRLDMVAALKTRE"
     gene            2624..3862
                     /locus_tag="PKV56_11625"
     CDS             2624..3862
                     /locus_tag="PKV56_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOX68507.1"
                     /translation="MTMKTGTKISIGVASLALAALLAWAFAPRPIEVEVAKARVGAFE
                     QTITEDGKTRLHQRYVVSAPLAGRLSRITLTEGDRVEANAVVATLTPVLSPMLDERSR
                     SEQNSRVEASEANVQRASARIARTRIALEQARNDLQRTELLAKQGFVAPIRLDNERLA
                     VQAAQREIDAAVQEHHVAQHELEQTRAALQASRGASGTAGAFELRSPIAGQVLRVVQT
                     SETTVAVGSPLIELGDLARMEIVVELLTTDALNAPPGSRVRIERWGGKTSLEGRVRLV
                     EPAAFTKVSALGVEEQRVRLLVDITSPPSEWQALGDAFRVEVHIVTVAIDQALMVPVS
                     AVFPLPAEARDADVNGRREQRFAVFVVDGGRARLKPVDVGARNGSQAWVRSGLKAGDT
                     VVIYPSPAVADAARVHPRAL"
     gene            complement(3929..4765)
                     /locus_tag="PKV56_11630"
     CDS             complement(3929..4765)
                     /locus_tag="PKV56_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX68508.1"
                     /translation="MDEIRSILLHLDASTGATRRLRAAHQLAATHGSELEVLYAVTPA
                     LLQYPMAASADSQLAGMLAALDVETRARVRADFERERLQVGLPHLAWNESDDTPLAAF
                     RRRAFEADLLVLAQVRPDDHQQSQVPVDFAPTVLIETGKPALLLPYIDTGSEFGQTVL
                     IAWKDSAASARAVTAALPVLRLAKSVHVAAWDESGSPGDGEPLPIERFLQRHGVHAKV
                     HRGGRPSGEVGELLLSLAADVQADLLVMGCYGHSRAREWLLGGVTRTIFQSMTLPVLM
                     SH"
     gene            complement(4949..6508)
                     /locus_tag="PKV56_11635"
     CDS             complement(4949..6508)
                     /locus_tag="PKV56_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68509.1"
                     /translation="MDTQRKSVTAVDNGMVVALRAHLQAMTGQPVELLETHISWILLS
                     GSLAYKLKKPVRLPFLDFTELESRQRYCELELRLNQRLAPSLYLDVVPICGTVDSPRI
                     DGIGEPIDYAVCMRRFAARALLSEQLADGHLHEAQVEQLTRRLADFHHDAPAAELGSQ
                     FGSPERISSAVANVIDQLDAVATASRMQAWRNWLKEQVRVLHPVWQSRRATGAVRECH
                     GDLHLANAVQVGEEVLAFDCIEFDPELRWIDVISDLSFLTMDLQAHGRADLAFVALDA
                     YLQRSGEYAGLQVLRFYEIYRALVRALVTSLQARNHIEGNVPGAPDYQGCAARMMPGS
                     RGGPRLMITRGLSGSGKSTVARRLLAQVGAVRVRSDVERKRLFGLGALQRSADHDLDL
                     YTPEATRRTFERLATCARDGLRAGYPVIVDAAFLRRGERQAFRALAAELRVPFSILDC
                     GASPDVLQQRLAARAAAGNDPSEADVSVLKRQVHWQEALDAHEQSRALEVATDQPVDY
                     VALGERWLAAT"
     gene            6758..7699
                     /locus_tag="PKV56_11640"
     CDS             6758..7699
                     /locus_tag="PKV56_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOX68510.1"
                     /translation="MEFKDYYALLGIERTATPEEIKRAYRKLARKFHPDVSKEADAEA
                     RFKDVSEAYEALSDPEKRAAYDEIGRRRERGQGYEPPPDGGFEFSGRSGGGPADDAAF
                     SDFFESIFGHRARGAPEPRHAGGAAADHHAKVQIDLEDAYRGARRTISLRMPVIDEQG
                     QAMLHERQLEVNIPKGVRDGQQLRLVGQGGPGQGGRPAGDLYLEVSIRPHAHFRLDGR
                     DLYLDLPITPWEGALGARVPVPTPDGALELGIPAGSWSGRKLRLKGKGLPGTTPGDLY
                     AVLQVTAPPVQTQADRDAYTALAQAHADFNPRSALMP"
     gene            7710..8036
                     /locus_tag="PKV56_11645"
     CDS             7710..8036
                     /locus_tag="PKV56_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HOX68511.1"
                     /translation="MNHTSDSRPLIVDAIVLDDSICFTLDTLCQACQSDRMQVTTLVH
                     EGVLDPVGSGPDDWHFAGSALRRARTALRLLRDLELGVVGTALVLDLLDEIDELNARL
                     RRAGLG"
     gene            8052..10742
                     /locus_tag="PKV56_11650"
     CDS             8052..10742
                     /locus_tag="PKV56_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HOX68512.1"
                     /translation="MSIRHLDRLLEPRSVAVIGASDRVGSVGATVWRNLRAGTFQGPI
                     HGVNPKRATLDGTVVYASPSDLPEVPDLAVLCTPPDTVPGLIDALGQLGTRAAIVMTA
                     GLSAAQKQAMRDAARPHLLRLLGPNCIGLLSPHIGLNASFAHTDAAAGDMAFVSQSGA
                     LVTAVLDWAKSRRIGFSHLVSLGERADVDFGDLLDHLASDARTRSILLYIESIESPRK
                     FMSAARAAARNKPVIVVKAGRAGHGMQAAASHTGAMAGSDIVFDAAIRRAGMLRVDTL
                     QDLFMAAETLAHFGHRQALTTSMPRPGDESLTLMTNGGGAGVMAADAAALAGVTLRDL
                     PDELVARLDAVLPVTWSRGNPVDIIGDAPVQRYTQTLQALLADGGAGTVLFMHAPTAI
                     VRSDDIARACAPLARQAAGRVMACWLGDWAVADARRIFVEAGVPDYATPEEAVSAFAL
                     LGTYRRNQTLLLEAPTASECDPPDIAAARLPIESALAAGREMLDELEAKAVLEAYGIP
                     TVRTVSVKPTAAAALEAAQTLGYPVVLKILSPDISHKSDVGGVALNLRDEVELGQAAE
                     GMLMRVGAAMPQARLAGFTVQTMASRPLAQELIVGASIDPLFGPVLLFGQGGTAVEVL
                     ADRAIALPPLNRVLARDLVSRTRVAKLLAGYRDHPPARIDAICDVLIALSQMLTDLPE
                     LAELDINPLWADHEGVIALDARIRLSRERPAGAAHFAIAPYPAELAESMAWQDESIVL
                     RPIRPEDEAQHRAFLDCLDANDLRLRFFSSRRELPRSELARLTQIDYAREMAFIAVRT
                     AADGTQQTLGVVRAVCDPDNIDAEFAVVVRSDLKARGLGQLLLRKMIAYLSAHGTQRM
                     VGRVMRENQPMLELVRNNGFVSDPATTDVDSLGIALNLPV"
     gene            complement(10807..11379)
                     /locus_tag="PKV56_11655"
     CDS             complement(10807..11379)
                     /locus_tag="PKV56_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68513.1"
                     /translation="MSCRTRLARWAALCWLAALCGCSALLPKGQTEDLSPFSKYEDAR
                     SSFVKVTPYRTTLQDLKALGFDAQASTNVREIAYPQFMSLVAESPYLHTDQLDAGIRD
                     CISAGARCRAYVFQFGHQSRNRTGGFWLDFFNFYRVTHTSGWRFQGVVLVRDDNLVLF
                     RNHSGEPNIEKTDVRRNPLGPLQSFGESMP"
     gene            complement(11487..12911)
                     /locus_tag="PKV56_11660"
     CDS             complement(11487..12911)
                     /locus_tag="PKV56_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOX68514.1"
                     /translation="MTLTHFNAWQCGATALDVWSSGSLSASALMQRQATRLSEMLRWA
                     ARRSPMYRALAGRVDPDRLTLDDFPVVTKPELMGKFDQWVTDPQLQIDELRRFIADRE
                     RIGEDHLGRYQVWESSGSTGEPGVFVQDAHALAVYDALEALRRPALQPLRRCVDPWYL
                     GERLAFVGATTGHFASTASIQRLRRLNPWMYRNMRSHSFMLPLPELVSALNDQAPTIL
                     TTYPSAALLLAQEASAGRLRIPVNEVWVGGESLSASTRECITRGLGCPVSQSYGASEF
                     LSLASECQHGRLHLNTDWAVLQPVDAQHRPVPVGETGFTTLLTNLANRVQPLIRYDLG
                     DRVRFAPQACACGSALPVIEVQGRVDDLLELDDALGRPVSLLPLALTTVLEDDAGVFD
                     FQLQQRSRHALWLCIAAEGADAAAALQRAQAVLRRFLSAQGLPNVSVGGNCGDRGQTG
                     RSGKRQRVVARTHRHRTCRVDAEP"
     gene            complement(13007..15460)
                     /locus_tag="PKV56_11665"
     CDS             complement(13007..15460)
                     /locus_tag="PKV56_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68515.1"
                     /translation="MTIVSVSPAQLRLNLEPSALGFADTSELLSLPLPWIGQERAEQA
                     ARFGLQMDQPDYNLFVLGDVGSGRTTLMTQMMSREAAGRAVPPDLCYLHNFEEPEHPR
                     ALRLPPGEGRLLRQLMADLVKTLQSEIPKRLLAGGVLAESERIENTCKAEEDRAFDEL
                     SAYAKSRHFGLLREEGRLVFTQCDEQGEPLTAGKALDLSREQRAAFDEAESALRTEIS
                     RFLEKTRARELAMNDSLAALRRQMIEPMLDHALQEIRNQLRKQIKDSVKLGQYLDQAH
                     DHVLENLELFQPGEDAEEMRVAALIEVLSRLRVNVAVDNHGLQGAPVIVDDNPLFRSL
                     FGSIAYASENDVLVTDFSRIRAGSLLKAHGGFLMLHLRDLLSDPQVWEKLRRFLRSGR
                     LQIEEPGMVFAPIAAVSLQPEPLDVDVKIILIASAEDYYAAQEGDPEIARRFRCKVDF
                     AESFMATPESRIATAIFVAHTCRKLGLPHFLPAAVCVLIESTHREAEDQARQSAIFAR
                     TEALVMESAAMARTRGALLVDAQDVQAAIDARVRRHGYPEQRLQESIVDGERLLQVTG
                     ERVGQVNGLTVVDLADYQFGFPVRVTASTHAGEEGLLNIEREVELSGPIHDKGVLILH
                     SYLYALFAHVAPLALNASVVFEQEYSGVEGDSASCAEFYALLSALSGLPLRQGIAVTG
                     AVNQHGEMLPVGGINEKIEGYFRSCELLGLDGQQGVLIPHRNRRHLMLSPRVVDAVSQ
                     GRFHVYTADMAGDGMELLTGRNFGTLSAGSYPADTVLGCAQRTLHAYREACQTSGTRR
                     QPSDWPRGPNPRRIRPGRR"
     gene            complement(15671..16054)
                     /locus_tag="PKV56_11670"
     CDS             complement(15671..16054)
                     /locus_tag="PKV56_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68516.1"
                     /translation="MAYQINWQERGVLYNYRGHTSDFEIAAATRIAQADARFDQLVYA
                     IHDFLPCESFTYSVPKIEEIAAVASAAALSLRAPRLAIAIVTDRPQVRAAADVYVESG
                     LTPHDLRVFPSIGEARGWLSSVHRP"
BASE COUNT         2657 a         5558 c         5565 g         2539 t
ORIGIN      
        1 cgcgccatcg tcaagcgccc cgaggtgctg ctgtgcgacg agccgaccgg cgcgctcgat
       61 tacgcgaccg gcaagctggt gctcgaggtc atcgagcgca tcaaccgcga gaccggcacc
      121 acggcggtcg tcatcacgca caacgcggcc attgccggca tggccgatcg cgtgatccac
      181 ctcggcgacg gacgaatcca gcgcgtcgag accaacgcgc acaagctgtc gccgtcgcaa
      241 ctgagctggt gaccatgaag gccctggatc gcaagctgat gcgcgacctg cgcgcgatgt
      301 ggagccaggt gctgaccatc gcgctggtgg tggccagcgg cgtggccgga ttcatcacca
      361 cgctgtctgc cgtcgattcg ctggccctgg cgcgcgaccg cttctacgcc gaggcgcgct
      421 tcgccgatgt gttcgcgggc ctcaagcgcg ctccgctgac ggtcgccgac acattgcgcg
      481 cgctgcccgg cgtggccgac gtgcagacca gcgtcgaagt ggccgtgcgg gtcgagatcg
      541 agggccgcag cgaaccgatc gtcgggcaac tgatcggcgt cgatccgcgt cggccggcgc
      601 gcatgaacct cgtcgtgctg cgcggcgccg ctgccgcgcc ctcgcccggc ccgccgctcg
      661 ccgatggatc gattccggcc tgggtgtcgg aatcgttcgc gacggccaac cagctgaagc
      721 ccggttcgcg gctgtcggcg ctgatcaacg gcaagcggcg cacgctgctg atgacgggcg
      781 tggcgctctc gcccgaatac gtcttcgccg gcctgtgggg catgcccgac cagcgcggct
      841 tcggtgtctt ctgggtcgac cacgaggcgc tggcggcggc gttcgacatg gccggcgcat
      901 tcaaccatct ggcgctgaag ctggcgccgc acggcgacga gcgcacggtg atcgacggcc
      961 tgaccggcgt gctcgacacc tacgggggcc ggcaggcgca tggccgcacc gaccagacct
     1021 cgcacgcgat gctcgacaac gagatcaagg agcagcgcgt gctgggcacg ctgctgccgt
     1081 cgatcttcct cggtgtcgca gcgttcctgc tcaacgtggt catcgcccgg ctggtctcca
     1141 cgcagcgaga gcagatcgcc gcgctgaagg ccctgggcta cccgaacgcg accatcgccc
     1201 tccactacct gaagatggtg ctggtcatcg tgagcatcgg ctggctgctg gggctggtga
     1261 tcggcgatca gctgggcgcc gcgctcacgc ggctgtactc ggacttcttc cgcttcccga
     1321 gcttcgagca ccgtgtcgcg ccctggctgc tcttggtcag cggcgggttg acgctggcga
     1381 ccgcggtgct cggcacgttg aatgcgatcg cggccacggc acgcctggcg cccgccgagg
     1441 cgatgcgccc gcaggcgccg ggccgctaca ggcgcacgct gttcgaacgg atgggcatac
     1501 gccacatccc caccagcctg cggatgatcc tgcgccagat ggaacgccgg ccggggcgca
     1561 ccgcgctcgc ggtcggcggc gttgccgcat cggtggcgat cgtcgtgatg gggaatttcc
     1621 tgcgcgacgc gatcgaatcg atcgtcgaca cccagttcac gctcgcgatg cgcaacgacg
     1681 tcgccgtgtg gacgctggat ccactgatcg acgcctcggg ccgcgagctg gcccggctgc
     1741 ccggggtcac cgcggtcgaa tcgaaccggt atgtcccggt cacgctggtg aacggtcatc
     1801 gtcgcgagcg cagccagctg cgcggctacg cgctgcagcc cgagctctac cggatcgtcg
     1861 acgtcaaccg ccggcagacg cagccgaccg gcgacgggct ggtgctcacc gacaggctgg
     1921 ccgacaagct cggggtccgg gtcggcgacc gggtgcaggt cgaggtgctc gaagggcgtg
     1981 cccgcacgct ggatctggtc gtggatgcca cggttcgcga aatgatggga ctgaacgcgt
     2041 acatgaaccg cccggcgctg aaccgggcgc tcggcgacgg cgatctctcc accggattca
     2101 tgctggccgt cgaacccggc cgcgaagccg aactgctgga cgccacgcgc cagctgccga
     2161 gggtggccgg cgcattcagc aaggcgacga tgctgcgcaa catgcaggaa ctcagcgccc
     2221 gcaatgtgcg catcatgagc acggtgctga cggtgttcgc gagcatcatt gcggtcgggg
     2281 tcgtctacaa caacgcccgc atcgcactgg cggagcgcac gtgggaactg gccagcctgc
     2341 gcgtgctcgg attcacgcgc gcggaggtct cgggactgct gctcggcgag atggtgatcg
     2401 tggtggcgct ggcgctgccg ctgggcatgc tgctcggatg ggggcttgtg catgtattgg
     2461 cgggggctct gaagagcgat cagttctact ttccggtggt gatcctgccg cgcacctacg
     2521 cctgggccgc gatctgcgtc gtcggcgccg cgctgggcag cgcgctggtg gtgcgccggc
     2581 gcatcgaccg gctcgacatg gtggccgcct tgaagacacg cgaatgacga tgaaaacagg
     2641 tacgaagatc tccatcgggg tggcctcgct ggcactggcg gcgctgctgg cctgggcgtt
     2701 cgccccgcgc cccatcgagg tggaagtcgc gaaggcccgc gtcggtgcgt tcgagcagac
     2761 gatcaccgag gacggcaaga cccgcctgca ccagcgctac gtggtgtcgg ccccgctggc
     2821 cggtcggctc tcgcgcatca cgctgaccga gggcgaccgg gtcgaggcaa acgccgtggt
     2881 cgcgacgctg accccggtgc tgtcgccgat gctcgacgag cgcagccgca gcgagcagaa
     2941 ctcccgcgtc gaagccagcg aggcgaacgt gcagcgtgca tcggcgcgca tcgcacgcac
     3001 gcgcatcgcg ctcgagcagg cccgcaacga tctgcagcgc accgagctgc tggcgaagca
     3061 gggtttcgtg gcgccgatca ggctggacaa cgaacgcctc gcggtgcagg ccgcgcagcg
     3121 ggagatcgat gcggcggtgc aggaacacca tgtcgcccag cacgagctcg aacagacgcg
     3181 tgccgcgctg caggcatcgc gcggcgcctc cggcacggcg ggtgccttcg agctgcgctc
     3241 gccgatcgcc ggtcaggtgc tgcgcgtggt gcagaccagc gagacgaccg tcgcagtcgg
     3301 atcaccgctg atcgaactgg gcgatctggc acgcatggag atcgtcgtcg aactgctgac
     3361 gacagacgca ctgaacgccc cgccgggcag ccgggtgcgc atcgagcgct gggggggcaa
     3421 gacgtcgctc gagggccgtg tgcgactcgt cgagcccgcc gcattcacca aggtgtcggc
     3481 actcggtgtc gaggagcagc gggtgcggtt gctggtcgac atcaccagcc cgccgtccga
     3541 gtggcaggcg ctgggagatg cattccgggt ggaggtgcac atcgtcacgg tggcgatcga
     3601 tcaggctctg atggtgccgg tcagcgcggt gtttccgttg ccggccgagg cccgcgacgc
     3661 cgacgtcaac ggcaggcgcg aacagcgatt cgccgtgttc gtcgtcgacg gcggacgggc
     3721 ccggctgaag cccgtcgacg tgggtgcccg caatggcagc caggcctggg tgcgaagcgg
     3781 actgaaggcg ggcgacacgg tggtgatcta tccgtcgcct gccgtggccg acgccgcgcg
     3841 ggtgcacccg cgcgcgctct gaacccgggc ctggaagcct cggacgcccg gcgccaggca
     3901 cgcccgcagc gccgagggcg gcagcggctc agtgcgacat cagcaccggc agcgtcatcg
     3961 actggaagat cgtgcgcgtg acgcctccga gcagccattc gcgggcccgg ctgtgtccgt
     4021 agcagcccat gaccagcaga tcggcctgca cgtcggcggc cagcgacagc agcagctcgc
     4081 cgacctcgcc gctcgggcgg ccaccgcgat ggaccttcgc atggacgccg tgccgctgca
     4141 ggaagcgctc tatcggcagc ggctcgccgt caccgggcga tcccgactca tcccaagcgg
     4201 ccacatgcac cgacttcgcc agccgcaaca cgggcaaggc agccgtcacg gcacgcgccg
     4261 acgccgccga gtccttccag gcgatcagca cggtctggcc gaactcggag ccggtatcga
     4321 tgtagggcag cagcaaggcg ggcttgccgg tctcgatcag caccgtcggt gcgaaatcga
     4381 cgggcacctg cgactgttga tgatcgtcgg gacggacctg cgccagcacc agcaggtcgg
     4441 cctcgaaggc ccgccgcctg aaggccgcca gtggcgtgtc gtcggactcg ttccaggcaa
     4501 ggtggggcaa tccgacctgc agtcgctcgc gctcgaagtc cgctcgcacc ctggcgcggg
     4561 tctcgacatc cagcgcggcc agcatgcccg cgagctggct gtcggcggag gccgccatcg
     4621 ggtactgcag cagcgctggt gtcaccgcgt acagcacctc gagctccgag ccgtgcgtgg
     4681 ccgccagctg gtgcgccgcg cgcaaccgcc gcgtggcacc ggtcgatgcg tccagatgca
     4741 gcaggatcga tctgatttca tccatgggga actcctggtg gttcgggcgg gcagcgcccg
     4801 ggtcggcggg agcggaaggc atcgccttcg caatccgcct gcagcaccac tgtaggcacc
     4861 gaccgcgaac cgcagttgcg cgggatcaag cgcaggccga cgggtcaccg atagcctgga
     4921 gtctgcactg ctgagccgca gtggccgctc aggtggcagc cagccagcgc tcgcccaggg
     4981 ccacgtagtc gacgggctgg tctgtcgcga cctcgagcgc ccgcgactgc tcgtgggcgt
     5041 caagagcttc ctgccagtgc acctgtcgtt tcagcaccga gacatcggcc tcggacggat
     5101 cgttgccggc agcggctcgc gcggccaggc gctgctgcag cacgtccgga ctggccccgc
     5161 aatcgaggat ggagaacggc acgcgcaact cggccgccag cgccctgaag gcctggcgtt
     5221 caccgcgtcg caggaacgcc gcgtcgacga tcaccggata gcctgcacgc agaccgtcgc
     5281 gggcgcaggt ggccagccgc tcgaaggtgc gccgggtcgc ctcgggcgtg tacaggtcga
     5341 gatcatggtc ggccgagcgc tgcagggcac cgagcccgaa cagccgcttg cgctcgacgt
     5401 ccgagcgcac acggaccgca ccgacctgcg cgagcagccg gcgggccacg gtcgacttgc
     5461 cggagcccga cagtccgcgg gtgatcatca gccgcggacc accccggctg cccggcatca
     5521 tccgcgctgc acagccctgg taatcgggtg cacccggaac gttgccttcg atgtggttgc
     5581 gcgcctgcag gctggtgacc agcgcacgca ccagggcccg gtagatctcg tagaagcgca
     5641 gcacctgcag gcccgcgtat tccccgctgc gctgcaggta ggcgtcgagc gcaacgaagg
     5701 ccaggtcggc tcgcccgtgc gcctgcaggt ccatggtcag gaacgacagg tcgctgatga
     5761 cgtcgatcca tcgcaactcc gggtcgaact cgatgcaatc gaacgccagc acctcctcac
     5821 cgacctgcac cgcgttggcc aggtgcaggt cgccgtggca ttcgcgcacg gcgcccgtgg
     5881 cgcgccgcga ctgccagacc gggtgcagca cccgcacctg ctccttcagc cagttccgcc
     5941 aggcctgcat tcgtgaagca gtcgcgacgg catcgagctg gtcgatcacg ttcgccaccg
     6001 cgctcgagat ccgttccggc gagccgaact gcgaaccaag ctcggcagcc ggggcgtcgt
     6061 ggtggaagtc ggccagtcgg cgggtgagct gttcgacctg cgcctcgtgc aggtgtccgt
     6121 cggccagctg ctcgctgagc agcgcgcggg ctgcgaagcg gcgcatgcac accgcgtagt
     6181 cgatgggctc gccgatgccg tcgatacgcg gcgaatccac ggtgccgcag atcggcacca
     6241 cgtcgaggta cagcgaaggc gcgagtcgct ggttcagccg cagttcgagc tcgcagtagc
     6301 gctggcgcga ctcgagttcg gtgaagtcga ggaacggcag ccgaaccggc ttcttcagct
     6361 tgtaggccag cgatccgctg agcaggatcc acgagatgtg cgtttccagc agttccaccg
     6421 gctggccggt catcgcctgc aggtgagcgc gcagggcgac gaccattccg ttgtccactg
     6481 cggtgacgga tttgcgctga gtgtccatcg aggcatgttt gcagtttgtt caggccgcaa
     6541 gcttgatctg cggcaaatca aggtgcacgg atcgtgcgcc acaacttggt tcgaccggcc
     6601 tcatgcagca tgggcaaaga atccccgggg attgcgcccc gtcaacgttg tgccgggtag
     6661 cggcacctac gatggtccga tcgggggcca cgccccggat gacctggcca cggtgaccac
     6721 ggcatcacgc agcgcgcgac aacaggcaga cacgcacatg gagttcaagg actactacgc
     6781 cctactcggc atcgagcgca ccgcaacgcc ggaggagatc aagcgcgcct atcgcaagct
     6841 ggcgcgcaag ttccacccgg acgtcagcaa ggaggctgac gccgaggccc ggttcaagga
     6901 tgtgtcggaa gcgtacgagg cactgagcga cccggagaag cgcgcggcct acgacgagat
     6961 cggccggcgc cgtgaacgcg gacaaggcta cgagcctcca cccgacggcg gcttcgaatt
     7021 cagcggacgc agcggtggcg gtcccgcgga cgatgccgcg ttcagcgact tcttcgagtc
     7081 gatcttcgga caccgcgcac gcggtgcccc cgagccgcgc cacgcaggtg gcgccgcagc
     7141 cgatcaccac gccaaggtgc agatcgacct cgaagacgcc taccgtggag cgcgccggac
     7201 gatctcgctg cgcatgccgg tgatcgacga gcagggtcag gcgatgctgc acgagcggca
     7261 gctggaggtg aacatcccga agggagttcg cgatggacag cagctgcgcc tggtcggaca
     7321 gggtggaccg ggacaaggtg gcaggccggc gggcgacctg tacctggaag tgtcgatccg
     7381 gccgcatgct catttccggc tcgacggccg cgacctgtac ctcgatctgc cgatcacgcc
     7441 gtgggaaggc gcgctcggcg cccgcgtgcc cgtgcccaca cccgacggcg ccctcgagct
     7501 cggcatcccc gccgggtcgt ggtcgggccg caagctgagg ctcaagggca agggtctgcc
     7561 cgggaccacc cccggcgatc tctacgcggt gctgcaggtc accgccccgc cggtgcagac
     7621 ccaggccgac cgcgatgcct acaccgcgtt ggcgcaagcc cacgccgact tcaatccgcg
     7681 cagcgccttg atgccgtgaa agagatgcga tgaaccacac ttccgattcc cggcccctga
     7741 tcgtcgatgc catcgtgctc gacgacagca tctgcttcac gctcgacacg ctctgccagg
     7801 cctgccagag cgaccgcatg caggtcacga cactggtcca cgaaggggtg ctcgaccccg
     7861 tcggcagcgg acccgacgac tggcatttcg ccggcagtgc gctgcgccgt gcgcgcactg
     7921 cgctgcgcct gctgcgcgat ctcgagctcg gcgtcgtggg taccgcgctg gtgctcgacc
     7981 tgctcgacga gatcgacgag ctgaacgccc gactgcgccg cgccggcctg ggctgacacc
     8041 cgccgccctg catgagcatc cgccatctcg atcgcctgct ggaaccccgc tcggtcgccg
     8101 tgatcggtgc atccgatcgc gtcggcagcg tcggagccac ggtgtggcgc aacctgcgtg
     8161 ccggcacctt ccaggggccg atccacggcg tgaatccgaa gcgcgcgacg ctcgacggca
     8221 cggtggttta cgcgagcccg tccgacctgc cggaagtgcc cgacctggcg gtcctgtgca
     8281 cgccgcccga caccgttccc ggcctgatcg acgcgctcgg ccaactcggc acgcgcgcgg
     8341 cgatcgtgat gaccgcgggg ctcagcgccg cacagaagca ggcgatgcgc gatgcggcgc
     8401 gcccgcatct gcttcgtctg ctggggccga actgcatcgg gctgctgagt cctcacatcg
     8461 gcttgaatgc cagctttgcg cacaccgatg ctgcagcggg agacatggcc ttcgtgtcgc
     8521 agtcgggggc gctggtcacg gccgtgctcg actgggcgaa atcgcggcgc atcgggttct
     8581 cgcatctggt ctcgctgggc gagcgggccg atgtcgattt cggcgatctg ctcgatcacc
     8641 tggccagcga tgcacgcaca cgatcgatcc tgctgtacat cgaatcgatc gaatcgccgc
     8701 gcaagttcat gtcggccgcc agggcggctg cgcgcaacaa gccggtcatc gtcgtgaagg
     8761 ccggccgcgc ggggcatggc atgcaggcgg ccgcatccca caccggcgcg atggctggct
     8821 cggacatcgt gttcgacgcc gcgatccggc gtgccggcat gctgcgtgtc gacacgctgc
     8881 aggatctgtt catggccgcc gaaaccctcg ctcacttcgg tcaccggcag gcgctgacga
     8941 cctcgatgcc gaggccgggc gacgaaagcc tgacgctgat gaccaatgga gggggcgccg
     9001 gcgtcatggc ggccgacgct gccgcgctgg ccggcgtcac gctgcgtgat ctgcccgacg
     9061 agctggtcgc tcggctcgat gcggtgctgc cggtgacctg gtcgcgaggc aacccggtgg
     9121 acatcatcgg cgatgccccg gtgcagcggt acacgcagac gctgcaggcg ctgctggccg
     9181 acgggggcgc aggcacggtg ctgttcatgc acgcgcccac ggccatcgtg cgcagcgacg
     9241 acatcgcgcg cgcctgcgcg ccgctcgcgc ggcaggccgc tgggcgcgtg atggcttgct
     9301 ggctgggcga ctgggcggtg gcggatgcgc ggcgcatctt cgtcgaggcg ggcgtgcccg
     9361 actacgccac gcccgaagag gcggtgagcg cgttcgcgct gctggggacc taccgccgca
     9421 accagacgct gctgctcgaa gcaccgaccg cgagcgagtg cgatccgccc gacatcgctg
     9481 ccgcgcggtt gccgatcgag tccgcgctgg ccgcgggccg cgagatgctc gacgagctcg
     9541 aagccaaggc ggtgctcgag gcctacggca tcccgaccgt ccgcaccgtg tccgtcaagc
     9601 cgacggccgc ggctgcgctg gaggccgcgc agaccctcgg ttacccggtc gtgctgaaga
     9661 tcctgtcgcc ggacatcagc cacaagtccg atgtgggcgg cgtggcgctg aacctgcgcg
     9721 acgaggtcga gctgggacag gccgccgagg gcatgctgat gcgggtgggt gcggcgatgc
     9781 cacaggcgcg cctcgcgggc ttcacggtgc agacgatggc gtcgcgacct ctcgcgcagg
     9841 agttgatcgt cggcgcgagc atcgacccgc tgttcggccc ggtgctgctg ttcggtcagg
     9901 gcggcacggc ggtcgaggtg ctcgccgatc gggcgatcgc actgccgccg ttgaaccgcg
     9961 tgctggcccg cgacctggtg tcgcgaacgc gggttgcgaa gctgctcgcc ggataccggg
    10021 atcacccgcc cgcgcggatc gacgccatct gcgatgtgct gatcgcgctg tcgcagatgc
    10081 tgaccgacct gcccgaactg gccgaactcg acatcaaccc gctgtgggca gatcacgaag
    10141 gcgtcatcgc gctcgatgcg cgcatacgcc tgagccgcga gcggccggct ggagcggcgc
    10201 acttcgccat cgcaccctac cctgccgaac tggctgaaag catggcctgg caggacgaat
    10261 cgatcgtgct gaggccgatc cgaccggagg acgaagccca gcaccgcgcc ttcctggact
    10321 gcctcgacgc gaacgacctg cgcttgcgct tcttcagttc ccggcgcgaa ctacctcgca
    10381 gcgagctggc tcgcctgact cagatcgact acgcgcgcga gatggccttc atcgcggtgc
    10441 gcacggccgc cgatggaacg cagcagacgc tgggggtggt gcgagcggtc tgcgaccccg
    10501 acaacatcga tgccgagttc gccgtcgtgg tgcgttcgga tctgaaggca cgcgggctcg
    10561 gccagctgct gctgcgcaag atgatcgcct acctgtcggc gcacgggacg caacgcatgg
    10621 tgggccgcgt gatgcgggag aaccagccca tgctcgagct cgtcaggaac aacgggttcg
    10681 tgtccgaccc ggctaccacc gacgtggatt ctctggggat cgccctcaac ctgccggtct
    10741 gaaccggcgg gatcatgtcg ggcacgggac ggtgatgcgt acccaccacc gccgatcgat
    10801 cgccggctag ggcatcgact ctccgaacga ctgcagcggg ccgagcgggt tgcggcgcac
    10861 gtcggtcttc tcgatgttcg gctcgccgga atggttgcgg aacagcacga ggttgtcgtc
    10921 acgcaccagc accacgccct ggaagcgcca gcccgaggtg tgcgtgacgc ggtagaagtt
    10981 gaagaagtcg agccagaaac cgccggtccg gttgcgcgac tggtgaccaa actggaacac
    11041 gtacgcgcgg caccgcgcgc ctgcgctgat gcaatcacgg atgccggcat cgagctggtc
    11101 cgtgtgcaga tacgggcttt cggcgacgag cgacatgaat tgcgggtacg cgatctcccg
    11161 cacgttcgtg ctcgcctgcg cgtcgaaacc cagcgccttc aggtcttgca gggtggtgcg
    11221 atacggagtg accttgacga aggaactgcg tgcatcctcg tatttcgaga agggagacag
    11281 gtcttcggtc tggcccttcg gcagcaacgc actgcacccg caaagcgctg ccagccagca
    11341 cagggcagcc catcgggcaa gacgcgttcg acaagacatg gagatacctg gttgttggac
    11401 agcaagccgg ccggcgcgac ttcgtgaaag cgcaagcctc ggagtgttcg atcccgcgtg
    11461 gcaatcgacg ttcccggggc accggttcac ggctcggcat cgacccggca cgtgcggtgt
    11521 cgatgagtgc gggccaccac gcgctgccgc ttgccactgc gtccggtctg cccgcgatct
    11581 ccgcagttgc ccccgaccga cacgttgggc agcccctgcg cgctcaggaa gcggcgcagc
    11641 accgcctgcg cccgctgcag cgcggcggca gcgtcggcac cctctgcagc gatgcacagc
    11701 cacagcgcgt ggcggctgcg ctgctgcaac tggaagtcga acacgccggc atcgtcttcc
    11761 agcaccgtgg tcaaggccag cggcagcagg ctcaccggac gcccgagcgc gtcgtcgagt
    11821 tccagcaggt cgtcgacccg gccctggacc tcgatcaccg gcaacgccga accgcatgcg
    11881 cacgcctgtg gagcaaacct gacgcggtcg ccgaggtcgt atcggatcaa cggctgcacc
    11941 cggttcgcca ggttggtcag caaggtcgtg aagccggtct caccgacggg aaccggacgg
    12001 tgctgcgcgt cgaccggctg caggaccgcc cagtcggtgt tcaggtgcaa tcgaccgtgc
    12061 tggcattccg atgccagcga cagaaactcg gacgctccgt agctctggct caccggacag
    12121 cccagccccc tcgtgatgca ctcccgggtc gaagcgctca gcgattcacc accgacccag
    12181 acctcgttca cgggaatgcg cagccgaccg gctgatgctt cctgggccag cagcagcgcg
    12241 gcgctcgggt aggtggtcag gatcgtcggc gcctgatcgt tgagcgccga caccagttcc
    12301 ggcagcggca gcatgaacga gtggctgcgc atgttgcgat acatccaggg gttgagccga
    12361 cgcaggcgct ggatcgatgc ggtgctggcg aaatggccgg tggtcgcgcc gacgaaggcc
    12421 aaccgctctc ccagatacca ggggtcgacg cagcggcgca gcggctgcag cgcaggtcgg
    12481 cgcagagcct ccagtgcgtc gtagaccgcc agggcgtgag cgtcctgcac gaatacgccg
    12541 ggctcgccgg tgctgccgga gctctcccag acctgatagc gccccaggtg atcctcaccg
    12601 atgcgctccc ggtcggcgat gaatcggcgc aactcgtcga tttgcagctg cgggtcggtc
    12661 acccactggt cgaatttccc catcagctcc ggcttggtca ccaccggaaa gtcgtccagg
    12721 gtcagtcgat ctggatcgac gcgtccggcc aaggcgcgat acatcggcga acgccgcgcc
    12781 gcccagcgca gcatctcgct cagcctcgtc gcctgacgct gcatcaacgc cgacgcggac
    12841 agtgatccgg acgaccagac atccagcgcg gtcgcgccgc attgccacgc gttgaaatgg
    12901 gtcaaggtca cgctccggtt ttccgcgtct gcgaatcttc gtaccgccct atcaggcgcc
    12961 tgccgcggta tcccgacatt gaccgtggtc aatcgcgggg cggcgttcag cgtcgaccgg
    13021 gtcggatgcg cctggggttc ggtccgcgcg gccaatccga cggttgcctg cgcgtgcccg
    13081 aggtctggca cgcctcgcga taagcgtgca gcgtccgctg cgcacagccc agaacggtgt
    13141 cggccgggta acttcctgcg ctgagggtgc cgaagttccg gcccgtcagc agctccatcc
    13201 cgtcgcctgc catgtccgcg gtgtagacgt ggaatcgacc ctgcgacacc gcgtcgacca
    13261 cccgcggact gagcatcagg tgacgccggt ttcgatgcgg gatcagcacg ccctgctggc
    13321 catcgaggcc gagcagttcg caactgcgga agtagccttc gatcttttcg ttgataccgc
    13381 ccaccggcag catctcgccg tgctggttga ccgcgccggt gaccgcgatg ccctgccgca
    13441 gcggcagacc cgacagggcg gacagcaggg catagaactc cgcgcacgag gccgaatcgc
    13501 cctcgacccc gctgtattcc tgctcgaaca cgacggaggc attcagcgca agcggcgcga
    13561 cgtgggcaaa cagcgcatag agatagctgt gcaggatcag cacccccttg tcgtggatgg
    13621 gacccgacaa ctcgacctca cgctcgatgt tgagcagccc ctcctctccg gcgtgggtgc
    13681 tcgcggtcac ccgcaccgga aaaccgaact ggtaatccgc caggtccaca accgtcagac
    13741 cgttcacctg accgacccgc tcgccggtca cctgaagcag tcgctcccca tcgacgatgg
    13801 attcctgaag gcgctgctcg ggatacccgt gacggcgcac gcgcgcgtcg atggcggcct
    13861 gcacgtcctg cgcgtcgacc agcagcgcgc cgcgcgtgcg cgccatggct gcgctttcca
    13921 tgaccagggc ctcggtgcgg gcgaatatgg cgctctgccg cgcctggtct tcggcctcgc
    13981 ggtgcgtcga ctcgatcagc acgcacaccg ccgcgggcag gaagtgcggc aatccgagct
    14041 tgcggcaggt gtgggccacg aagatcgcgg tcgcgatgcg cgattcgggt gtcgccatga
    14101 agctctcggc gaaatcgacc ttgcagcgaa agcgccgggc gatctcgggg tcgccttcct
    14161 gcgccgcgta gtagtcctcg gccgaggcga tcaggatgat cttcacgtcg acatccagcg
    14221 gctccggctg cagcgacacc gcggcgatcg gcgcgaagac catccccggc tcctcgatct
    14281 ggagccggcc gctgcgcagg aagcggcgca gcttctccca cacctgcgga tcggaaagca
    14341 ggtcgcgcag atgcagcatc aggaagccac cgtgagcctt cagcaagctg ccagcgcgga
    14401 tgcgcgagaa gtccgtcacc agcacatcgt tctcggatgc gtacgcgatg ctgccgaaca
    14461 gcgaacgaaa cagcgggttg tcgtcgacga tgaccggagc gccctgcaag ccgtggttgt
    14521 ccacggcgac gttcacgcgc agacgggaca gcacctcgat cagcgcggcg actcgcattt
    14581 cctcggcgtc ttcgccgggc tggaacagtt cgaggttctc cagaacatgg tcgtgcgcct
    14641 ggtcgaggta ctggcccagc ttgaccgagt ccttgatctg cttgcgcagc tggttgcgta
    14701 tctcctgcag cgcgtggtcg agcatcggct cgatcatctg gcgccgcagg gccgccaggc
    14761 tgtcgttcat ggcgagctcg cgggccctcg tcttctcgag aaaccggctg atctcggtgc
    14821 gcagcgcgct ctcggcctca tcgaacgcag cccgctgctc gcgactcaga tccagcgcct
    14881 tgcccgccgt gagcggctcc ccctgctcat cgcactgggt gaacaccagt cgcccctcct
    14941 cgcgcaacaa gccgaaatgg cgcgacttcg cataggcgct cagttcgtcg aaggcacggt
    15001 cctcctcggc cttgcaggtg ttctcgatgc gctcgctttc ggcgaggacc ccgccagcca
    15061 acagccgctt gggaatctcg gactgcagcg tcttgaccag gtcggccatc agctggcgca
    15121 gcaggcgacc ctcgccgggg ggcaaccgca gtgcacgcgg gtgttcgggt tcttcgaagt
    15181 tgtgcaggta gcagaggtcg ggtggaaccg ctcgtccggc cgcctccctg gacatcatct
    15241 gggtcatcag cgtggtgcgg ccgctgccca cgtctcccag cacgaacagg ttgtagtcgg
    15301 gctgatccat ctgcaggccg aagcgcgcgg cctgttcggc acgctcctgg ccaatccacg
    15361 gcaacggcag ggacagcaac tcggaggtgt ccgcgaagcc cagggccgaa ggctcgagat
    15421 tcagtcgcag ctgggctggg gacacactga cgatggtcat gggcgcactc gttgcttctc
    15481 tggaccattg tgcgactgcc ggatgcactc ggcatccacg ggttgcccaa ccacaggacg
    15541 aggcgtcgac tttcggccgc tgtgtcaccg atcggtccga cggccgcagg ctgaccgctt
    15601 cgcaactcgg gcggcgccaa ggccatggat gcggttcagg ctggtgtggc cttgacccgc
    15661 tcccgaaaac ctacggccga tgaacgctgc tcaaccagcc acgagcttcg ccgatcgaag
    15721 gaaagacgcg caggtcgtgc ggggtcagac cggactccac gtagacatcg gcggccgcgc
    15781 ggacttgcgg ccggtccgtc acgatcgcga tcgccagcct gggcgccctc agcgacaggg
    15841 cggctgccga ggcgactgca gcgatctcct cgatcttcgg caccgagtac gtgaagctct
    15901 cgcagggaag gaagtcgtgg atggcataga ccagttgatc gaaccgggcg tctgcctgcg
    15961 cgatgcgcgt ggccgctgcg atttcgaaat cgctcgtgtg gccgcggtag ttgtacagga
    16021 caccgcgttc ttgccaattg atctgatacg ccatgggacg gaagtgtggc tcagatctcc
    16081 tagctcaatc attatttgaa caaaacaaac ataaatatca atataaactg ataacaatga
    16141 cacggacgca gacctccggc cagctctcgc gaaggctaga cccgctcgct aaatcgaggc
    16201 gcggcgttcc tgagagagcc agctgagcac ggtctcgaac agatcccgcg cctcgatcgg
    16261 cttcgtgatg aagtcgtcca tgccggcggc caggcagcgt tcgcggtcct gctcgaacg
//
LOCUS       DAOORV010000082        15956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4456_length_15956_cov_8.177725, whole genome shotgun sequence.
ACCESSION   DAOORV010000082 DAOORV010000000
VERSION     DAOORV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4456_length_15956_cov_8.177725"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKV56_11675"
     CDS             <1..944
                     /locus_tag="PKV56_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOX68517.1"
                     /translation="KLRYHRIIIMTDADVDGAHIRTLLLTFLFRQMPELVERGHIYIA
                     QPPLYKVKFGKEEQYLKDGTELDAYLLRVALKDAQLDTGIEGSAVLQGETLETLARQY
                     VLANNVVDRLSAWMDIEALRAMANGLHLDLDTLEAAEQSALALKDALHDAEVTAEFDA
                     RLDKHILRISRMHHGNVKSSVITADFVHGADYDALSTAGRTFKGLVSGAATVKRGEGE
                     RQKQATVGDFRQAMAWLIQQAESAVARQRYKGLGEMNPSQLWETTMDPTVRRLLRVQI
                     DDAIEADRVFTMLMGDEVEPRREFIETNALRVANIDV"
     gene            966..1802
                     /gene="dinD"
                     /locus_tag="PKV56_11680"
     CDS             966..1802
                     /gene="dinD"
                     /locus_tag="PKV56_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HOX68518.1"
                     /translation="MKKEIIVRLHASFEELVQTDETGDEYWLARDLQELLGYAKWENF
                     AKVIERAMTACQGAGYEPLDHFLEVRKMVDLGSGATRAIDDIALTRYACYLIAQNGDP
                     SKDAIAFAQTYFAVQTRKQELIEKRLAEVERLSARKKLTFSEKELSGIIYERVGDQMS
                     FARIRSRGDVALFGGHTTQDMKSKLGVPEHRPLADFLPTITIKAKDFANEITNFNIKR
                     DGLRHEDGISQEHVKNNKDVRALLVKRGIHPEALPPAEDIKKIERRVQSEAKKLPAKK
                     KR"
     gene            1856..2407
                     /locus_tag="PKV56_11685"
     CDS             1856..2407
                     /locus_tag="PKV56_11685"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX68519.1"
                     /translation="MSKTIYYLPGRGGRIATGLGEGLLNRGFDVTGRETVGEFRSRVF
                     QEQVDIVANDLTTDFWNKDARVVANSFGAYLFLHAQAQIESYVGNVLLLSPIVGECSN
                     EETLVNFIPPRAGKLQEMAQSGAYPAPQNCEIHVGEQDWQSVPTNVIALAQQLGLSVT
                     VVPGAGHVLGTDYVTSVLDKWLR"
     gene            complement(2429..3292)
                     /locus_tag="PKV56_11690"
     CDS             complement(2429..3292)
                     /locus_tag="PKV56_11690"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOX68520.1"
                     /translation="MQSDSNSPDRIQTILGIDAAWSETNPSGVALVQKRGPDWHCIRV
                     ASSYASFVSGQLEETVNCSKVTMRRIPNADLLIETATGLAGVQPDLVCIDMPMATTPI
                     KKRRVADSCVSKMYGARWCAAHSPSAQRPGAISNKLREDLGNLGYPLATADMVAGTTR
                     ALIEVYPHPAIVEFLRLQRRLPYKVSKAHTYWPGFSTGEKRENLLVEFHRLHDALTLV
                     FGPLELPLPAVGSSGSLASLKRFEDALDALVCCWVGVHYLGGQKQAVALGDPESAIWC
                     PIVPTLSGSNA"
     gene            complement(3301..4077)
                     /locus_tag="PKV56_11695"
     CDS             complement(3301..4077)
                     /locus_tag="PKV56_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68521.1"
                     /translation="MKLNALSRMPVPDWLAHVSIEARPPMAELLEGSVYYPACGFDGR
                     PVQYIAGYSHSFIYVDYGCPAKEVVAALAQDQAFLGYRIVGGRFLDSKELIGPCAVQQ
                     IELDRKLDGDPNRCRDHWVDPYAYWAVFERDSDLSEAHGPHKFSLIYIAGDGVVTYQN
                     LYFSYRARPSVVAIIQPGESFGFNWTNFYQPTQIFARTVSATERGGPSYLLIGGRGAS
                     MEFQQHIRWPQYGDLLRFWKSSDGYLALWEHSAKCSQIDG"
     gene            complement(4230..4419)
                     /locus_tag="PKV56_11700"
                     /pseudo
     CDS             complement(4230..4419)
                     /locus_tag="PKV56_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            4652..5599
                     /locus_tag="PKV56_11705"
     CDS             4652..5599
                     /locus_tag="PKV56_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOX68522.1"
                     /translation="MPRILTLTMNPALDVSTSIAKVVPAHKLRCAASIQHPGGGGVNV
                     ARVLQRLGCDCQALYLAGGANGQVLRRLLDGEQLRQQVIDISAETRESFSVHETSSGM
                     DYRFVLPGPTVQRHEWEACLAYIAALDAPPRYLVASGGLPPGVPTDFYARVARLAGER
                     GTRLILDTSGPALAAALAEGVYLFKPSLRELRGLSGLPLTTEAEWREASRQIVQAGRA
                     HIVVLSLGEEGALLVSADCVLRSQGLHVQVASTIGAGDSLVAGLVWALDQGHALEEAL
                     RCGMAAGAAALLSAGTALCDPADVRRLREEVVITIDAKS"
     gene            5920..6375
                     /locus_tag="PKV56_11710"
     CDS             5920..6375
                     /locus_tag="PKV56_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOX68523.1"
                     /translation="MKKTLISLALTLGATLAVQAKDIVDTAVAAGNFKTLATALQAAG
                     LVDTLKGKGPFTVFAPTDEAFAKIPKDQLDALLKDKAKLTSVLTYHVVPGKVMAKDVK
                     AGKVKTVQGSELTVTTTGGVKVDNANVTTTDIVADNGVIHVIDSVVLPK"
     gene            complement(6430..6792)
                     /locus_tag="PKV56_11715"
     CDS             complement(6430..6792)
                     /locus_tag="PKV56_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68524.1"
                     /translation="MTPAKRPPRPAHRPGRHHHHVYVVELDDRVWNEPRFRKANPDHQ
                     LGKPFVYVGMTGLEPDLRFDKHKAGIQANRYVFEYGLRLLPALYAVYNPMPYEAAREM
                     EVELAIGLREAGYGVWQA"
     gene            complement(6789..7031)
                     /locus_tag="PKV56_11720"
     CDS             complement(6789..7031)
                     /locus_tag="PKV56_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02450 family Trp-rich protein"
                     /protein_id="HOX68525.1"
                     /translation="MAQNTPPKNPLSPKKLLLTKWTAAVPRHKEKHFVVIRVVEPEPP
                     AVRIEQVELEAVYSKRAFLLHWRELTDASQWHQGWV"
     gene            complement(7093..7602)
                     /locus_tag="PKV56_11725"
     CDS             complement(7093..7602)
                     /locus_tag="PKV56_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HOX68526.1"
                     /translation="MTPAQPGDLYRFDTAEAVRAWRAIDDRVMGGVSRSGLRHDPAGH
                     ATFEGEVSLEQNGGFASVRSAPSPLGVSGALDCVIEARGDGKRYKLCLFVVDAFDALS
                     YQAEFVPAAHAWDVISLPIAAFTATTRGRRVADAPALDPARIRQVGLMISGRQAGRFA
                     LDIRSIRLG"
     gene            8036..8998
                     /locus_tag="PKV56_11730"
     CDS             8036..8998
                     /locus_tag="PKV56_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOX68527.1"
                     /translation="MTPSETRAIVTVSLLAAFADGDKHDRERTEIKRIAEGLSQADGI
                     HLPTLYQDVLMKRVTLPSVVPALASNESRQLAYEMAVCVCDADGAQSVAEQAFLAELG
                     RALQISAPAAAEFRQQAEAMASVPLSEGATVVAATGTSGASVPAASIDHAGLDAAILN
                     AAIVNGALELLPETLSTMAIIPLQMKLVYRIGQAHGYELDSGHVKDFLATAGVGLTSQ
                     YLEQAGRKLLGGLLGKLGGGLLRGVGNQAVSSGMSFVSTYALGHVAKRYYAGGRTLSA
                     QMLKDAYASVTQQAQGLYSQHLPAIQQRASTLDAGKVMAMVRGA"
     gene            complement(9014..9997)
                     /locus_tag="PKV56_11735"
     CDS             complement(9014..9997)
                     /locus_tag="PKV56_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOX68528.1"
                     /translation="MKRRNLLIASLGLGSTLLTRAAAENRPDLQWRTRPLLALGTTVR
                     LRAAHVDAAQADRALHAAAATLQAVEASMSLYREDSELSRLNRTGWLHAPSAHLLNVM
                     REARLVSQRSGGAFDVTVQPLWSAYERARQAGRLPTDAERIDAQARTGWQQVQLDRER
                     VRFLRSGMAITLNGIAQGYAADAVRATLQAHGVEHALIDAGEFGAIGRNDRGQPWNVG
                     VEDPHDSSRVLAALALNGRALATSADNRSIFSADRRHHHIIDPSTGDSPPALSSVTVV
                     AASAMRADALTKVMFMAGPGRITALARQWDVGVLWVDKQGQWEATPGLLPA"
     gene            complement(10012..12198)
                     /locus_tag="PKV56_11740"
     CDS             complement(10012..12198)
                     /locus_tag="PKV56_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX68529.1"
                     /translation="MRPRVTPPLRALLDRVGRIFRALALFAFSALLALSASASTLTRE
                     RLSELFPLPLTVGERDATLPVWPLFKNDLTSTVLVGYVFESIDFAPVPGYSGTPVNLL
                     IALDAKGVFLDVKVLSHHEPVFLEGLGEVPLFRFVDQYKGLALQQSIKIGSNQNRGGQ
                     VGSANVYIDGVSKATASVRIINQSLLSASLAIARAKLGFAAGRDPDLIGRVKGDLYEA
                     LDFAALERKGLVTQRVWRNSDIETAFKGTVGEGQDPVALQSPDAVFSEMLVAHLNVPS
                     VGRNLLTPAAWDYLTGWLEPGDHAFLVVTRGRYSFMGDDYAIGAVPDRLTLGQGGLPL
                     EIRDFNVEGRPRLPEAWLGPDARWRIFKVINQASLDPSQPLDFALSVTRSKGQILPER
                     ERKDFTLRAQLPPAYADQPRSNEKTWHSIWSGRAWELGLLVVALAGLSLVLARPGWLV
                     QSTRRLEQIRLAWLAFTLVFIGWYAQGQLSIVNFTALIHSLMAGRSLDFFLYDPMTVL
                     LWAFVAVSLIAWGRGTFCGWLCPFGALQEFVAKLARRVRLPQLKLHTRIDARLKKLKY
                     VVLVGIVATAFVSPAWTDRLVEVEPFKTSITLMFDRSWPYLLWAVGLLALNAVVYKAY
                     CRYLCPLGAGLAVLGRIRLLNWLPRRAECGQPCQTCRHKCEYQAIEPVGKIDYAECFQ
                     CMDCVAIHDSDQLCAPRIMATRKGRVIPVHALPPGPAHPPAMHVSP"
     gene            12382..13260
                     /gene="ubiA"
                     /locus_tag="PKV56_11745"
     CDS             12382..13260
                     /gene="ubiA"
                     /locus_tag="PKV56_11745"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOX68530.1"
                     /translation="MSGPTVSPSRLSLYLDLIRWNRPAGSYLLLWPTLSALWMAAGGF
                     PGWHLLVVFTLGTVLMRSAGCTVNDVADREFDRHVKRTAQRPVTRGAISAREALLFGA
                     ALAVAAFALVLTTNLSTILWSFAALAVAVFYPYTKRFFAMPQAVLGVAFSFGIPMAFS
                     AVRGDVPALAWGLLLGNLFWVLAYDTEYAMVDRDDDLKIGIRTSAITLGRFDVAAVMG
                     FYSLYLALWAVLGARQGLGVLYFAGVGAAAGMAAWHYALIRDRSRDGCFRAFRLNHWV
                     GFAVFAGLLLDHGLRA"
     gene            complement(13195..15156)
                     /locus_tag="PKV56_11750"
     CDS             complement(13195..15156)
                     /locus_tag="PKV56_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68531.1"
                     /translation="MSGLRALATRLLPQRLGRRLQLLVSLGLGLTVSLFGAWTIHEQS
                     ALALSSIETEASALARNIALSSENPILTDQLDVIETLLIRAADFPQLLDVRITDAQGE
                     SLGHVARSLGGTPRVLFDPPGSRIVLPPRASPLSNHDGSGNRVVAWHPIVAGTLLGWV
                     RLERSTQALGALRNRIWFGTIVAGLLAAASSAALLGWLLRSSVRSIGRSKDFAVGLAR
                     IDGQQIGSLEGSIETRELGIALNQASTRLFEQRRAIDAGMATLQLQEAVLADANEQLS
                     TIFALSPDALLSFDSAGRVRFANEAFFRISGLGAPAVIGQPAAALDAEFRARCADPQA
                     YPGLESFFAATDTIKSGPEGRFVRPRLTLSRPRLQVLEVVGLLSHSPSVSRLLYVRDV
                     THESEVDRMKSEFLTTAAHELRTPMTTINGCIELLQAREFDSARRKELLGMANRQSQL
                     MVTIIDQLLDYARIEARRGADFVTERLRLADLLAQALSDFVPPAGRLAPELLDADGAG
                     WVQVDRGKTLQVVRNLLSNAYKYSTSGAVQVHLLARRSGAAGDLFGFQVSDHGIGMTP
                     EQLARVCERFYRADASGHVPGTGLGMSIVQELVSLMGGTLELASVPDQGTTVTVWLPC
                     APTDSRAISGSQTVIEQQAGEHRETHPVV"
     gene            complement(15167..>15956)
                     /locus_tag="PKV56_11755"
     CDS             complement(15167..>15956)
                     /locus_tag="PKV56_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX68532.1"
                     /translation="VLGAAPERNSYSLSVVPQFPASEIHRDWTPLLERLAKDTGITLS
                     MTLSANIPRFEADLLDGHPDFAYMNPYHQVMAARAQGYIPLVRSSKSLSGILVVRKDS
                     PIASVRELDGKEVAFPAPNAFGASLWMRALLAERERINIKPVYVQTHSNVYRHVIRGK
                     AAAGGGINSTLLQEQPEVQSDLRVLLDTPGAAPHPLSAHPRVPARVQQLVASALLSLT
                     ADAAGQALLKDVFGGVPVRADYARDYRPLEQFKLEKYVVTDTPG"
BASE COUNT         2849 a         5092 c         5241 g         2774 t
ORIGIN      
        1 acaagctgcg ctaccaccgc atcatcatca tgaccgacgc cgacgtggac ggtgctcaca
       61 tccgcacgct gctgctgacc ttcctgttcc gccagatgcc cgaactggtt gagcgtggcc
      121 acatctacat cgcgcagccg ccgctctaca aggtcaagtt cggcaaggag gagcagtacc
      181 tgaaggacgg caccgaactc gacgcctacc tgctgcgcgt ggcgctgaag gatgcccagc
      241 tcgataccgg catcgaagga tcggccgtgc tgcagggcga gacgctggaa acgctggcgc
      301 ggcagtacgt gctggccaac aacgtggtcg atcggctgag cgcgtggatg gacatcgagg
      361 cgctgcgcgc gatggccaac ggcctgcacc tcgacctcga cacgctcgag gccgccgagc
      421 aatcggccct ggcgctgaag gacgcgctgc acgacgccga agtcaccgcc gaattcgatg
      481 cccgcctcga caagcacatc ctgcgcatca gccgcatgca tcacggcaac gtgaagagca
      541 gcgtgatcac cgccgacttc gtgcacggtg ccgattacga cgcgctgagc accgccggcc
      601 gaactttcaa gggcttggtg agtggcgcag ccaccgtcaa gcgcggcgag ggcgagcgcc
      661 agaagcaggc gaccgtcggc gacttccgtc aggcgatggc gtggctgatc cagcaggccg
      721 aatcagccgt ggcgcgccag cgctacaagg gcctgggcga gatgaacccc agccagctct
      781 gggaaaccac gatggacccg accgtgcgcc gcctgctgcg cgtgcagatc gatgacgcca
      841 tcgaagccga tcgcgtgttc acgatgctga tgggcgacga ggtcgagccg cgccgcgagt
      901 tcatcgagac gaatgcgctg agggtggcga atatcgacgt gtgattcagt gaggagcttc
      961 ttggcatgaa gaaggaaatc atcgtcaggc ttcatgcgag ctttgaagaa ctcgttcaga
     1021 ccgacgagac cggtgacgag tactggcttg cgcgtgatct tcaggagctg ctgggttatg
     1081 cgaagtggga gaacttcgcc aaggtgatcg agcgggcgat gactgcctgc caaggtgctg
     1141 gatacgaacc gctggaccat tttcttgagg tcaggaaaat ggtcgatctg ggcagcggcg
     1201 ctacccgcgc catcgacgac atcgcactca cccgctatgc ctgctacctg atcgcgcaga
     1261 acggcgatcc cagcaaggac gcgatcgcgt ttgcacagac ctatttcgcc gtacagacgc
     1321 gcaagcagga gttgatcgaa aagcgccttg ccgaagtcga acgcctgagc gctcgcaaga
     1381 agctgacgtt ctcggagaag gaactgtcgg gaatcatcta cgagcgggtc ggcgaccaga
     1441 tgagttttgc tcgcattcgc agtcgcgggg atgtggcctt attcggtggg cacaccaccc
     1501 aagacatgaa gagcaagttg ggcgttcctg agcaccgtcc cttggccgac tttctgccga
     1561 cgatcaccat caaggccaaa gactttgcga acgagatcac caacttcaac atcaagcgcg
     1621 acggcctgcg ccacgaagac ggcatctcac aagagcatgt gaagaacaac aaggacgtgc
     1681 gggcgttgct ggtgaagcgg ggcatccatc ctgaggccct gccacccgcc gaagacatca
     1741 agaagattga gcgccgggtt cagtctgagg ccaagaagtt gcctgcaaag aagaagcgct
     1801 gacatccacg tgcgcggtga ttgatgttcg caattcaaga ttcgctaaaa gcgacatgag
     1861 caaaacgatt tactacctgc ctggccgtgg cgggcgcatt gcgacaggac ttggcgaggg
     1921 cctcctcaat agaggctttg acgtcacagg tcgggaaacc gttggcgagt tcagaagccg
     1981 tgtttttcaa gagcaagtcg acatcgttgc caacgatctg acaactgact tttggaataa
     2041 agatgcccga gttgttgcca attcattcgg cgcttacttg ttcctccatg cgcaggcaca
     2101 aatagagtcg tacgttggga acgtgttgct gttgtctcca attgtcggtg agtgctcgaa
     2161 cgaagaaaca ttggtgaact tcattccacc gagggctgga aaactccaag agatggctca
     2221 aagcggtgct tacccagccc cgcaaaactg cgagattcat gtcggcgaac aggactggca
     2281 aagcgtacct acaaacgtca tagctcttgc ccagcagctt gggttgagcg tgacagttgt
     2341 ccctggggcg gggcatgtcc ttggaacaga ctatgtgact tctgttttgg acaaatggct
     2401 tcgctgagca gtcatcatga atccggcctc acgcattgct tccactgagg gtcggaacaa
     2461 tggggcacca gatggcagat tctgggtcgc ccagcgccac tgcctgcttt tgtcctccca
     2521 agtaatggac gccaacccag cagcacacaa gcgcgtcgag cgcatcctcg aagcgtttca
     2581 gactggccag cgagccactg cttccgactg cgggcaatgg aagttccaat ggtccgaaga
     2641 ccaaggtcag cgcatcgtga aggcgatgaa actcaaccag caggttttct cgcttttccc
     2701 ccgtagagaa accaggccaa tacgtgtgag cctttgacac cttgtagggg agcctccgct
     2761 ggagtcgcag gaattcgaca attgccggat ggggatagac ctcgatcaag gcgcgcgtcg
     2821 taccagccac catgtccgca gtcgccaagg gatagcccag attgcccaag tcttcccgca
     2881 gcttgttgct gatcgcaccc ggtcgttgcg ccgatggaga gtgcgcagcg caccatcgag
     2941 ccccatacat ttttgatacg catgagtcgg caacgcgtct cttcttgata ggcgttgtcg
     3001 ccattggcat atcgatgcaa acgaggtccg gttgaacgcc ggccaagcca gtggccgttt
     3061 caattagcaa atcagcgttt ggaatacgac gcatggttac ctttgagcaa ttgaccgtct
     3121 cttccaattg cccactaaca aacgacgcgt aactcgaagc gacgcggatg caatgccaat
     3181 ctgggccgcg cttttgaacc aacgcaacac cactcgggtt cgtctcgctc caggcggcgt
     3241 cgataccgag aatggtttgg attcggtctg gtgaattgct gtctgattgc ataccggtga
     3301 ctacccgtcg atctgactgc acttcgctga atgttcccag agagccaaat agccgtctga
     3361 ggatttccag aacctcaaca agtcgccgta ctgtggccaa cgaatgtgtt gctgaaattc
     3421 catgctcgca cctcggcccc caatcagcag gtaactcggc ccacctcgct ctgtggctga
     3481 aacagttcgc gcaaatatct gggtcggttg gtagaagttg gtccaattga aaccgaagct
     3541 ctctcctggc tggatgatgg cgaccacaga gggtcgagcc ctgtaagaga aatagagatt
     3601 ctggtaggtc acgacaccat ctccagcgat gtaaatcaag ctgaatttgt gtggcccatg
     3661 ggcctctgac aagtcggaat cccgctcaaa taccgcccag tatgcgtagg gatcgaccca
     3721 atggtctcgg caacggttcg gatcgccatc cagttttcga tccaactcga tctgctggac
     3781 tgcacagggt ccgatgagtt ccttactgtc gagaaatcgt ccgcctacga tccgatagcc
     3841 aagaaaggct tggtcttgcg cgagcgccgc aaccacttcc ttggctgggc aaccgtagtc
     3901 gacatagata aagctatggc tgtagccggc aatgtactga actggacggc cgtcaaaccc
     3961 acaagcgggg taatagacgg agccttcgag cagttctgcc atgggcggac gggcttcgat
     4021 cgacacatgc gcaagccaat cgggtacggg cattcgggac agggcgttga gcttcattca
     4081 ggactcgatt tggagcgttg ttttcccggt caacggattg aaaggcggag ccgctcggag
     4141 cagagggcgc gcggcatcaa ggccgccaca ctccatcccc cgcctcctac agcccgatcg
     4201 ccagctccac cttcatctcc cacgccgaca tcaatttgcg ctaccgccag ccgctgctta
     4261 gccgccatat cacggtcggc cgaaaaatcc tgccgcagcg cctgctcgat ctcgaactgc
     4321 caggtgtgct ggccctcgat gcggttggag tagtacgagt tcatcccccc gcaacaaccg
     4381 tcgcaactcg gcaggcaccg acgtgccggt caactgcaat gcagggcaag ccagccgtgc
     4441 ccggatgaac gatagtcgag tgcaaggact tcgacgctgc gattggccag tcgcaacaac
     4501 ccatgcagac cacagatttt caccttggcg cgcccctcgc caagtcgttg acggagcccc
     4561 acgtgccaga gcaactgatc tggcgccctg cggattcact ggcgttctgc gcgcggcttg
     4621 atccggcagc gggcccaacc accgatctgc catgccccgg atcctgaccc tcaccatgaa
     4681 tccggcgctg gacgtgtcga cgtccatcgc caaggtggtt ccagcgcaca agcttcggtg
     4741 cgcagcctcc atccagcatc ccggtggagg gggcgtcaac gtcgccaggg tgctccagcg
     4801 tctggggtgc gactgtcaag cgctctacct ggcaggtgga gcgaacgggc aggtactgcg
     4861 gcgcctgctc gacggcgagc aactgcgcca gcaggtcatc gacatttcgg ccgagacgcg
     4921 cgagagtttt tcggtacacg aaacctccag cggcatggat taccgttttg ttctccccgg
     4981 gccgacggtt cagcgccacg aatgggaggc ttgtctggcc tacatcgctg cgctcgatgc
     5041 gccgcctcgc tatctggtgg ccagtggtgg actgcctccg ggcgtcccga cggatttcta
     5101 tgcccgcgtg gcgagactgg ccggcgagcg aggcacccgg ctgattctgg atacctccgg
     5161 cccggcgctg gccgccgcac tggctgaagg cgtgtacctc ttcaagccga gcctgcgtga
     5221 gctgcgaggc ctgagcggac ttcccttgac cacagaagcc gagtggcgcg aggcgtcccg
     5281 acagatcgtt caggccggtc gagcccacat cgtggtgctg tcgctgggag aggagggcgc
     5341 cttgctcgtt tcagcggact gcgttttgag gtcgcagggc ttgcatgtgc aggtggccag
     5401 caccatcggt gcgggtgaca gcctggtcgc aggtctggtc tgggcgctgg atcaggggca
     5461 tgcgctggag gaggcgcttc gctgcggcat ggctgcagga gcagcagcac tgctgtcagc
     5521 gggcacggcg ctgtgcgatc cggcagatgt caggaggctg cgcgaagagg tggtgatcac
     5581 catcgacgcg aagtcctgat cgccagctcc gcgccgggtc gcgggagggt tgttgccgca
     5641 ccctccctca tgcccgccac aacccatgac ccgcacggtc tcggatggcg tctggtttgc
     5701 agaccgcgtg cagcgggcca tcgcgggcgg aggctgccgc tcggcagtgc gaaacgaagg
     5761 gggctggtga ggaatgccaa ggagtaacaa aaatactcgc cggtatattt tgtgaacttg
     5821 ttggttgcgc ttctggtcag catcactccc gtctgctgtt tccgagcggg gtcgataccc
     5881 gtcctcgata agagggccat caaccaaggc agtaccacca tgaaaaagac tctgatttct
     5941 ctcgcgttga cactcggtgc cacgttggcc gttcaggcca aggacatcgt tgacacggcc
     6001 gttgccgctg gcaacttcaa gacgctggca acggcactgc aggcggccgg cctggtcgac
     6061 accctgaagg gcaagggccc gttcactgtg ttcgcaccga ccgacgaggc tttcgccaag
     6121 atcccgaaag accagcttga cgcactgctc aaggacaagg ccaaactgac ctccgtgctc
     6181 acgtaccacg tggttccggg caaggtgatg gccaaggacg tcaaggcagg caaggtcaag
     6241 acggtacagg gcagtgagct gaccgtgacc accaccggag gcgtcaaggt cgacaacgcc
     6301 aatgtgacca ccacggacat cgtggcggac aacggcgtga ttcacgtcat cgacagcgtc
     6361 gtgctgccga agtaaggcgg gtcgagcggc gggcttttca gctcgctgcc cgcatttcgg
     6421 cgctgcccct cacgcctgcc acaccccata ccccgcttcc cgcaacccga tcgccagctc
     6481 cacttccatc tcccgcgccg cctcgtaagg catcgggttg tagaccgcgt acagcgccgg
     6541 tagcaggcgc aggccgtact cgaagacgta gcggttcgcc tggatgccgg ccttgtgctt
     6601 gtcgaatcgc aggtcgggct cgaggcccgt catgccgacg tagacgaatg gcttgccaag
     6661 ctggtggtcg ggatttgcct tgcggaagcg gggctcgttc cacacgcgat cgtcaagctc
     6721 gacgacgtag acatggtggt ggtgccggcc cgggcggtgc gcgggccgcg gcgggcgttt
     6781 tgccggggtc acacccaacc ctggtgccat tggctggcgt cggtcagctc gcgccagtgc
     6841 agcaggaagg cccgcttcga gtacacggcc tccagctcca cctgttcgat gcgcaccgcg
     6901 ggcggttcgg gttcgaccac gcggatcacc acgaagtgct tttccttgtg gcgcggcacc
     6961 gcggcggtcc acttggtcag cagcagcttc ttggggctga gcgggttctt cggcggtgtg
     7021 ttctgcgcca cctgagtgtg gggctggtct ctgtctgcgc gcgtcatggt tgccgatgat
     7081 gcccggcccc gttcacccga gccggatcga acggatatcg agcgcgaaac gccccgcctg
     7141 gcggcccgag atcatcagtc cgacctgacg gatgcgggcc gggtccagcg cgggcgcatc
     7201 ggctacccgc cgcccgcgag tcgttgccgt gaacgcggcg atcggcaagc tgatgacgtc
     7261 ccatgcgtgc gcggctggaa cgaactcggc ttggtagctc agcgcatcga aggcatcgac
     7321 cacgaacagg cagagcttgt agcgcttgcc atcgccgcgg gcctcgatga cgcaatcgag
     7381 ggcgcccgac acgccgagcg ggctgggtgc agaacgcacg ctggcgaaac cgccgttctg
     7441 ttccagcgac acctcgccct cgaaggtggc atgaccggcg gggtcgtgcc tgagaccgct
     7501 gcgagacacg ccgcccatca cccggtcgtc gatggcccgc cacgcacgca ccgcttcggc
     7561 ggtgtcgaat cggtacaggt cgccgggctg tgcgggggtc atcctcgaat tttcaactag
     7621 cgccggtatc tgcgtgagtc gtctctcgaa tggtcatgcc cgcgatcgtg atcggcgctg
     7681 tggtcgcgat gctcgccgtg ccggtgaccg ccgtggtcac cccaccacag aggtcgaacg
     7741 acgcatccgc tgagagatgc tccaacgatc aacacgatgc cgatcctgga aaggtggcga
     7801 ctgaaattca tctggttctc cttggcgacc tgcagggtca cctggatcac caacgtggcc
     7861 ttcggtgccg tgttgaccgc caacgaccgg tgcgaggtaa agctcaggta aagaccgggt
     7921 cctcggcgac ggccctgcct cgctccagcg ggccgcgccg gtgctctggc gccacgcaag
     7981 gaaccttcgg ccacgccggc aaccgaaccg gtctgcccca gtcctggaac ccgacatgac
     8041 cccctccgaa acccgagcca tcgtcaccgt cagcttgctc gccgccttcg ccgacggcga
     8101 caagcacgac cgggagcgca ccgagatcaa gcgcattgcc gagggcctgt cacaggccga
     8161 tggcatccac ctgcccacgc tctaccagga cgtgctgatg aagcgggtga cgctgccatc
     8221 ggtggtgcct gcgctggcca gcaacgagtc gcgacagctg gcctacgaga tggcggtgtg
     8281 cgtctgcgac gccgatggtg cgcagtcggt ggccgagcag gcgttcctcg ccgaactggg
     8341 ccgcgccctg cagatcagcg ctcccgcggc cgccgagttc cggcagcagg ccgaagcgat
     8401 ggcctcggtg ccgttgtccg aaggcgccac tgtcgtcgcg gcgacgggca cctccggcgc
     8461 ttccgtgccg gctgcgagca tcgaccacgc ggggctcgac gccgccatcc tgaatgccgc
     8521 gatcgtcaac ggcgcgctcg agctcctgcc ggagacgctg tcgacgatgg cgatcattcc
     8581 gctgcagatg aagctggtct accgcatcgg ccaggcgcac ggctacgaac tcgacagcgg
     8641 ccatgtgaag gactttctgg ccactgccgg tgtcggcctg acctcgcagt acctggagca
     8701 ggccggccgc aagctgctcg gcggcctgct gggcaagttg ggtggcgggc tgctgcgcgg
     8761 tgtcggcaac caggctgtca gttcgggcat gagcttcgtc tcgacctatg cactggggca
     8821 tgtggccaag cgctactacg cgggcggccg cacgctgtcc gcgcagatgc tcaaggacgc
     8881 ctatgcgagc gtgacgcagc aggcgcaggg cctgtacagc cagcacctgc cggccatcca
     8941 gcaacgcgcg agcacgctgg atgccggcaa ggtgatggcg atggtgcgcg gagcctgagc
     9001 ccgaccggct cgatcaggcc ggcaacagac cgggagtcgc ttcccattga ccctgcttgt
     9061 cgacccacag cacacccaca tcccactgcc gagccagtgc cgtgatccgg ccgggaccgg
     9121 ccatgaacat caccttggtc agcgcgtcgg cgcgcatcgc gctcgcggcg accacggtca
     9181 cgctgctcag cgcgggtggt gaatcgccgg tggacggatc gatgatgtgg tggtgccggc
     9241 ggtcggcgct gaagatgctg cggttgtcgg ccgaggtggc cagcgcgcgc ccgttcagtg
     9301 ccagcgcagc cagcactcga gaggagtcgt gcggatcctc gacgccgacg ttccagggct
     9361 ggccgcggtc attgcggccg atggcaccga attcgccggc gtcgatcagc gcatgttcga
     9421 cgccgtgggc ctgcagcgtg gcgcgcaccg cgtcggccgc gtagccctgg gcgatgccgt
     9481 tgagggtgat cgccatgccg gaccgcagga agcgcacgcg ttcgcggtcg agctgcacct
     9541 gctgccagcc ggtgcgtgcc tgtgcatcga tgcgttcggc gtcggtcggc agtcgaccgg
     9601 cctgacgcgc tcgctcgtag gcggaccaca gcggttgcac cgtcacgtcg aaagcaccgc
     9661 cgctgcgctg cgacaccagc cgcgcctcgc gcatcacgtt cagcaggtgg gcgctcggcg
     9721 cgtgcagcca gccggtgcgg ttcagccggc tgagctcgct gtcctcgcgg tagaggctca
     9781 tcgatgcctc cacggcctgc agcgtcgccg ctgcggcgtg gagcgcgcgg tcggcctgtg
     9841 ccgcgtcgac atgcgctgcg cgcagccgca ccgtggtgcc cagcgccagc agcgggcggg
     9901 tgcgccattg aaggtcgggt cggttctcgg cggccgcgcg agtcagcagc gtgctgccca
     9961 ggcccaggct cgcgatcagc aggttgcgtc gcttcatcgg tgttcccttc ttcatggcga
    10021 gacgtgcatg gccggtgggt gcgccgggcc cggcggcaac gcgtgcaccg ggatcacgcg
    10081 gcccttgcgc gtggccatga tgcgcggtgc gcagagctgg tcgctgtcgt ggatggccac
    10141 gcagtccatg cactggaagc actcggcgta gtcgatcttt ccgacgggtt cgatggcctg
    10201 gtactcgcac ttgtggcggc aggtctggca gggctggccg cattcggctc ggcgcggcag
    10261 ccagttcagc agccggatgc ggcccagcac cgccagtccg gcgcccagcg ggcacaggta
    10321 gcggcagtag gccttgtaga ccacggcgtt gagtgccagc agcccgaccg cccacagcag
    10381 gtagggccac gagcggtcga acatcagcgt gatcgaggtc ttgaacggct cgacctcgac
    10441 cagccggtcg gtccatgccg ggctgacgaa agccgtcgcg acgatcccga ccaacacgac
    10501 gtacttcaat ttcttcaagc gagcatcgat gcgggtgtgc agcttgagct gcggcaggcg
    10561 gacccgccgg gcgagcttgg cgacaaactc ctgcagtgcg ccgaacgggc acagccagcc
    10621 gcagaaagtg ccgcgccccc aggcgatcag gctgaccgcg acgaaggccc acagcagcac
    10681 ggtcatcggg tcgtacagga agaagtcgag gctgcgcccg gccatcagtg aatgtatcag
    10741 cgcggtgaaa ttgacgatcg agagctgacc ctgcgcatac cagccgatga acaccagcgt
    10801 gaacgcgagc cacgcgagcc gaatctgctc cagcctccgc gtcgattgca ccagccagcc
    10861 cggacgggcc agcaccaggc tcaggccggc cagcgccacc accagcagcc ccagttccca
    10921 ggcacggccg gaccagatcg aatgccaggt cttctcgttg ctgcgcggct ggtcggcgta
    10981 ggcgggcgga agttgcgcgc gcagcgtgaa atccttgcgc tcgcgctcgg gcaggatctg
    11041 ccccttgctg cgcgtcacgc tcaacgcgaa gtccagcggt tgcgaggggt cgaggctggc
    11101 ctggttgatc accttgaaga tgcgccagcg cgcatcaggc ccgagccagg cttcgggcag
    11161 gcgcgggcgg ccctcgacgt tgaagtcgcg gatttccagc ggcaggccgc cctgaccgag
    11221 cgtgagccga tcgggcaccg cgccgatcgc gtagtcgtct cccatgaagc tgtagcgacc
    11281 gcgcgtgacc accaggaatg catggtcgcc gggctcgagc cagccggtga ggtagtccca
    11341 cgcagcgggc gtcagcaggt tgcgcccgac gctcggcacg ttgaggtgcg cgaccagcat
    11401 ctcgctgaac acggcatcgg gcgactgcag cgcgaccggg tcctgcccct cgccgacggt
    11461 gcccttgaaa gccgtctcga tatcgctgtt gcgccagacc cgctgtgtga ccagaccctt
    11521 gcgctccagc gcggcgaagt ccagcgcctc gtagaggtcg cccttgacgc ggccgatcag
    11581 gtcgggatcc cgtccggcgg caaagcccag cttggcgcga gcgatggcca gcgacgccga
    11641 cagcagactc tgattgatga tgcgcaccga ggcggtcgcc ttggacacgc cgtcgatgta
    11701 gacgttggcg ctgcccacct ggccgccccg gttctggttg gagccgatct tgatgctctg
    11761 ctgcagcgcc agacccttgt actggtcgac gaagcggaac agcggcacct cgccgagacc
    11821 ctccaggaac accggttcgt gatgcgacag caccttgacg tcgaggaaga cgcccttggc
    11881 gtccagcgcg atgagcaggt tgaccggcgt gccggaatag ccgggtaccg gcgcgaagtc
    11941 gatcgactcg aacacatagc cgaccagcac cgtgctggtc aggtcgttct tgaacagcgg
    12001 ccacaccggc agcgtcgcgt cgcgttcacc gaccgtcagc ggcaagggaa agagttcgct
    12061 gagccgctcc ctggtgagcg tgctcgccga agccgaaagg gcgagcaggg ccgagaaggc
    12121 gaacagcgcg agtgccctga agattcgacc cacccggtcc agcaaggcgc gcaggggggg
    12181 tgtgacacga gggcgcatga aagtgctcag cattgaaaca ggaggggttc caccggcaat
    12241 cgccgggcca tgccggtgtc gtcgtctggg gtccggtgga ttgtcgtccc ggtgacgcga
    12301 ggttgcggcc cgctccgggt gaacaccgag gcgccttggc gacaatgtcg ggtgtcccct
    12361 tttcgtgatg cacacgctcc cgtgtccggc cccaccgtct ccccctcgcg cttgtcgctt
    12421 tacctggacc tgatccgctg gaaccgaccg gccggcagct acttgctgct gtggcccacg
    12481 ctgtcggcgt tgtggatggc ggcaggcggg ttcccgggct ggcacctgct ggtggtgttc
    12541 acgctcggca cggtcctgat gcgcagcgcc ggctgcacag tcaacgacgt ggcggatcgt
    12601 gagttcgacc gccacgtcaa gcgcaccgcg cagcgcccgg tgacccgtgg tgcgatctcg
    12661 gcgcgcgagg cgcttctgtt cggcgcagcg ctggcagtcg cggcgttcgc gctggttctg
    12721 acgaccaacc tctcgaccat cctgtggagc ttcgccgcgc tggccgttgc ggtgttctat
    12781 ccctacacca agcgcttctt cgcgatgccg caggcggtgc tgggggtggc gttcagtttc
    12841 ggcatcccga tggcgttctc ggccgtgcgc ggcgacgtgc ccgcgctggc gtgggggctg
    12901 cttctgggca atctgttctg ggtgctggcg tacgacaccg agtacgcgat ggtcgaccgc
    12961 gacgatgacc tgaagatcgg tatccgcacc tcggcgatca cgctcggccg attcgacgtt
    13021 gccgcggtga tggggttcta ctcgctgtac ctggcgctct gggccgtgct tggcgcgcga
    13081 cagggtctcg gcgttctgta ctttgcgggc gtcggggcgg ccgcgggcat ggcggcctgg
    13141 cattacgcac tgatccgcga ccgttcgcgc gacggctgct tccgcgcctt tcgcctaaac
    13201 cactgggtgg gtttcgcggt gttcgccggc ttgctgctcg atcacggttt gcgagcctga
    13261 gatcgcgcgc gaatccgtcg gtgcgcaggg cagccagacc gtgaccgtgg tgccctggtc
    13321 cggcacgctc gccagttcga gggtgccgcc catcagcgag acgagttcct gcacgatgct
    13381 catgccgagg ccggtacccg gcacgtgacc ggacgcgtcg gcgcggtaga agcgttcgca
    13441 gacccgagcc agctgttccg gcgtcatgcc gatgccgtga tcgctgacct ggaagccgaa
    13501 gagatcgcca gcggcgccgc tgcgccgcgc gagcagatgc acctgaacgg cccctgacgt
    13561 ggagtacttg tacgcgttgg acagcaggtt gcgcaccacc tgcagcgtct tgccgcgatc
    13621 gacctgcacc catcccgcac cgtcggcgtc cagcagctcg ggggcgagac ggccggcggg
    13681 tggcacgaag tcgctcaggg cctgtgcgag caggtccgca agccggagcc gttcggtcac
    13741 gaaatcggcg ccccgtctcg cctcgatgcg agcgtagtcg agcagctggt cgatgatcgt
    13801 gaccatcagc tggctctgcc gattggccat gccgagcagc tccttccggc gcgcgctgtc
    13861 gaattcgcga gcctgcagca gttcgatgca gccgttgatg gtggtcatcg gcgtgcgcaa
    13921 ctcgtgagcc gccgtggtca ggaactcgct tttcatgcgg tccacttcgc tttcgtgggt
    13981 cacatcgcgc acgtagagca aacggctgac cgacggcgaa tgactgagca gacccacgac
    14041 ctccaggacc tgcaatcggg gtcgggagag cgtcagccga gggcgcacga accggccctc
    14101 ggggccgctc ttgatcgtgt ctgtggctgc gaagaagctt tcgagcccgg ggtaggcctg
    14161 aggatctgca cagcgcgcac ggaactcggc atcgagcgcg gcagccggct ggccgatgac
    14221 cgcgggtgcg ccgagcccgc tgatgcggaa gaacgcctcg ttggcaaagc ggacgcggcc
    14281 ggccgagtcg aacgacagca gggcgtccgg gctgagcgcg aagatcgtgc tcagctgctc
    14341 gttggcatcg gcgagcacgg cctcctgcag ttgcagcgtc gccatgcccg catcgatggc
    14401 ccgccgctgc tcgaacagcc tcgtcgaggc ctggttgagc gcgatgccca gctcgcgggt
    14461 ctcgatcgag ccctcgaggc tgccgatctg ctggccgtcg atgcgggcca gcccgacggc
    14521 gaaatccttc gaccgaccga tggagcgaac cgacgagcgc aacaaccacc ccagcagcgc
    14581 agcgctgctg gccgcggcaa gcaatcccgc gacgatggtc ccgaaccaga tgcggttacg
    14641 cagggcaccc agcgcttgcg tgctgcgttc cagccgcacc cagccgagca gcgtgccggc
    14701 gacgatcggg tgccaggcga cgacacgatt cccactgccg tcgtgatttg acagggggct
    14761 tgcccgtggc gggagcacga tgcggctgcc cggcggatcg aacaacacgc ggggcgtacc
    14821 ccccaggctg cgggccacgt ggcccaggct ctcgccttgc gcgtccgtga tgcggacatc
    14881 cagcaactgc gggaagtcgg cggcgcgaat cagcagggtt tcgatgacgt cgagttgatc
    14941 ggtcaggatc gggttctcgc tggacagggc gatgttgcgc gccagcgccg aagcctcggt
    15001 ctcgatgctg ctcagggcca gcgcagactg ctcgtggatc gtccaggcgc cgaacagcga
    15061 aacggtcaga ccgagcccca gggacaccag cagctgcagc cggcggccga gccgctgagg
    15121 caacagccgc gtcgcgaggg ctcgcaatcc ggacatgagt gctcggtcag ccgggcgtgt
    15181 cggtgacgac gtacttctcg agcttgaatt gctcgagcgg gcggtagtcg cgcgcgtagt
    15241 cggcacgcac cggaactcca ccgaagacat ccttgagcaa ggcctgaccc gcggcgtcgg
    15301 cagtcagcga gagcagtgcg ctggccacca gctgctgcac ccgcgccggc acgcgcggat
    15361 gggccgacag cgggtgcggg gcggcccctg gcgtgtcgag cagcacgcgc aggtcggact
    15421 ggacctccgg ctgctcctgc agcagggtgc tgttgatgcc gccgcccgca gcggccttgc
    15481 ctcggatcac atgccggtag acgttgctgt gcgtctgcac atagacgggc ttgatgttga
    15541 tgcgttcccg ctcggcaagc agcgcccgca tccacagtga ggcaccgaac gcgttgggag
    15601 caggaaacgc aacttccttg ccgtcgagtt cgcgcacgct ggcgatcggg ctgtccttgc
    15661 gaaccaccag gatgccgctg agcgacttgc tgcttctcac cagcgggatg tagccctgcg
    15721 ccctggctgc catcacctgg tggtaggggt tcatgtaggc gaaatcgggg tggccgtcga
    15781 gcaggtccgc ttcgaatcgg ggaatgttcg ccgagagggt catgctgagc gtgatgccgg
    15841 tgtccttggc caggcgctcg agcagcgggg tccagtcacg gtggatttcc gaggccggaa
    15901 actgcggcac caccgaaagg ctgtacgagt tgcgctcggg cgcggcgccg aggacc
//
LOCUS       DAOORV010000083        15831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4500_length_15831_cov_7.129754, whole genome shotgun sequence.
ACCESSION   DAOORV010000083 DAOORV010000000
VERSION     DAOORV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15831
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_15831_cov_7.129754"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKV56_11760"
     CDS             <1..1018
                     /locus_tag="PKV56_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOX68533.1"
                     /translation="SIQSECPIGLIGDDIEAVSKKKSKEYEGHTIVPVRCEGFRGVSQ
                     SLGHHLANDAIRDWVFDKLDGSKRAEFESTPYDVTIIGDYNIGGDAWSSRILLEEIGL
                     RVIAQWSGDGTIAELENTPKAKLNVLHCYRSMNYISRHMEEKYGVPWVEYNFFGPTMI
                     EKSLREIATHFDDTIKANAEKVIAKYKALTDAVIAKYKPRLQGKKVMLFVGGLRPRHV
                     IGAYEDLGMDVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFVEKVQPDLI
                     GSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAISSPVWALTKAPW
                     KKAA"
     gene            1112..2671
                     /gene="nifK"
                     /locus_tag="PKV56_11765"
     CDS             1112..2671
                     /gene="nifK"
                     /locus_tag="PKV56_11765"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HOX68534.1"
                     /translation="MPQNADKVIDHEMLFREPEYQELFANKKKDFEFNHSDDRIAEIR
                     DWTKSKEYKDKNFAREALTVNPAKACQPLGAVYVANGFHKTMSFVHGSQGCVAYYRSH
                     FSRHFKEPTSCVSSSMTEDAAVFGGLNNMIDGLANTFNLYKPDMIAVSTTCMAEVIGD
                     DLNAFIKTSKEKGSVPAEFDVPFAHTPAFVGSHVTGYDNALLGILQHFWDGKAGTTPA
                     LERVADESINFIGGFDGFVVGNMKEIKRIFDLFGCDYNVLCDPSETWNTPTDGEFRMY
                     EGGTTKETVIRALNAKATIVFQEYSCEKTIKYLKEKGQEVVVLNSPIGVAGTDAFVMA
                     LSRLTGKDVPAQLEKERGQLVDAMADSQAHLHGKRYALYGDPDMMLGLTQFLLELGAE
                     PAHVLATNGNEGWAAKVQALFDASPYGKGCQVYPKRDLWHMRSLLHTEPVDFLIGNTY
                     GKYLERDTGIPLIRMVFPIFDRHHYHRYPIWGYEGALRTLVMFLDEFFETLDANTIVP
                     GKTDYSYDIIR"
     gene            2767..3093
                     /locus_tag="PKV56_11770"
     CDS             2767..3093
                     /locus_tag="PKV56_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68535.1"
                     /translation="MTVPEPVYVKHLSDDRVVAVIGRHVCIDGQPEADHLVVIDEHPN
                     RQAILRAVPGATHVAGRVPLTMPQASMAQAALRRAHDSFDGSARAVNERLRNAVWQKV
                     FADGVE"
     gene            3128..3613
                     /locus_tag="PKV56_11775"
     CDS             3128..3613
                     /locus_tag="PKV56_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68536.1"
                     /translation="MIYVVEIPAQGDAHAWFAFNEDDLLRKVAADDRRAVHEIWDSAT
                     ARELLDLFDEQPDTAGVEERYPGIHRLGIEFGWDAPLFRADYLQEPGLYVPEPTRLID
                     ACRAALRARGGEWRFYEGEQHALTAVDKPDELYDRPGGWRARWALRQQLIAVEALADD
                     L"
     gene            3603..4160
                     /gene="hscB"
                     /locus_tag="PKV56_11780"
     CDS             3603..4160
                     /gene="hscB"
                     /locus_tag="PKV56_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOX68537.1"
                     /translation="MTSEAGTLQVDVGVPIDATDFELFEVAPRFVQDRAVLDARWKWL
                     QTQVHPDRFVTHGAVAQRVAMQWTLRVNEAYQRLRVPLSRAVYLCELNGQSIGAESNT
                     AMPAEFLMQQMAWREALDEAPNLETVEALADEVAERQRTLLDDLAIVMDELSDWPAAV
                     VQIRALMFVTRFAEDVERRADALGG"
     gene            4605..5306
                     /locus_tag="PKV56_11785"
     CDS             4605..5306
                     /locus_tag="PKV56_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX68538.1"
                     /translation="MRRFGTFAADGKHRLLARGLAVAALWIGAWAIAPAQAADIQGDA
                     AAGAQKNAMCIGCHGIENFKTGFPIVHRVPMLHGQNAQYIAAALAEYKVGDRKHPSMR
                     AVAGTLSEQDMADLGAYFESAGPRKIDPPVATTNDPAVALLNRGGCQSCHGANFNKPI
                     NPAYPKLAGQHADYLYVALKAYQQTEPKPFIGRGNPIMQSMVKQFSANELQTLADYLE
                     SLPGDVVTVQRSRFR"
     gene            5391..6257
                     /gene="coxB"
                     /locus_tag="PKV56_11790"
     CDS             5391..6257
                     /gene="coxB"
                     /locus_tag="PKV56_11790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOX68539.1"
                     /translation="MNTPKRIAFGVAAATAPAVSMADYSFNFPDPVTPIAHETLHVHN
                     LFMIVILVIFFAVFGIMIYSMWAHRKSRGHAAAGFVAPRSGKQWFWVSVPFAALLVID
                     YGVFGVPAYHAILSYEDTRTEADMVVKITGNQWLWQYEYPKEGIQFSSRLTTPRAQID
                     NEEAKGEHYLLEVDNPLVLPVGKKIRFITTSNDVIHSWWVPAFGVKRDAVPGFLREFW
                     AKVDRPGTYRGQCSELCGKEHGFMPIVVHVLPEAEFNAWLAAQKAGSAAAPADASAPA
                     PETPAADAQTKS"
     gene            6291..7880
                     /locus_tag="PKV56_11795"
     CDS             6291..7880
                     /locus_tag="PKV56_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HOX68540.1"
                     /translation="MSDATTIDAHDHYTPGGMLRWISTTNHKDIGLMYLIFGITMLVT
                     GGLMILGVRAELFKPGLQLMQPEFFNQLITLHGLIMVFGFAMPVATGLANYMLPMTLG
                     APDMALPRLNNWGFWILPPAAILLVLPFFLGIFGIGPGAVDAGWTMYAPLSIQSGWGM
                     DFAIFGIHMLGVSSILGSINVIVTILNMRAPGMTLMKMPLFAHGFLVTAILLITVMPV
                     LAGGVTMMLMDRHFGTHFFNAAGGGDPVLWQHIFWFMGHPEVYVLFLPIAGAIPHVLT
                     AFTRKETYGYRAQVYSMWAIGALSVIVWAHHMFTSGMSTNAQLYFMYSTMLISVPLAV
                     LFFCWIATLWRGSMSFETPMLFAIGSIVLFGWGGLTGLVLADAAADPQYHNAYFLVAH
                     FHYALYPTTALGSMAAIYFWLPKWTGHMINERLGKLHFWIAMIGFNLTFIPQFMVGLA
                     GMPRRIPDYPLMFQEWNMLSSIGAFILGASHLIFIYNIYRAYQGGPKAEAKSWEGAQG
                     LEWTLPSPAPYHSFGTQPVIK"
     gene            8071..8613
                     /locus_tag="PKV56_11800"
     CDS             8071..8613
                     /locus_tag="PKV56_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOX68541.1"
                     /translation="MNETDGQRRERGNRRLIVKLAIVVAFFSGFGFAMVPLYDAFCRI
                     TGLNGRTNAAAMPVVANTQVDMARSIRVEFLSHSMPGVGLSLKPETFSIHVNPGAIIH
                     TTYEVRNITDRVYVGQAVPSITPATATQYFQKIECFCFEKQTLQPGEVRTMPVVFVVR
                     PELPDALRTITLSYTFFQAV"
     gene            8668..9531
                     /locus_tag="PKV56_11805"
     CDS             8668..9531
                     /locus_tag="PKV56_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOX68542.1"
                     /translation="MSHTPSPGTHAHYFVPEVSRYSLPLSVFIFLLAAGYVLRINDLS
                     AGIWGMLLGAAGILFVVVAWFGVVISENIRGVYTGWEDRTYRIGMVWFIFSEVMFFAC
                     FFGALYYVRGIALPELGSYEPGLTPYSGFTGAWPSSGPLGDKFTPMGAWGIPALNTAL
                     LLTSGATLTWAHWGLLKGNRFQLNLGLALTVALGVAFLFFQVEEYMHAYSELGLTLGA
                     GVYGATFFMLTGFHGFHVTLGTIMLIVMLGRGLRGHFSKDNHFAFEAVAWYWHFVDIV
                     WLLLFVLVYWL"
     gene            9546..10280
                     /locus_tag="PKV56_11810"
     CDS             9546..10280
                     /locus_tag="PKV56_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOX68543.1"
                     /translation="MLLRRWIVPALATVLGAGLFTYLGLWQKGKAERVESTIAQYELR
                     AQQPPEIIGPARIDPAQAANARFTVRGRYEPEHQFFVDNRQEAGQAGVHVVTPLRIDQ
                     SDVRILINRGWIGWPGGRRVLPEVAVPGGIVEIIGTAQVPSTRESLWTPDRQEAAANL
                     WSRVDLARYAATSGHAVQPVVLLQDSGNANDGLVRHWPAPENRAPMHWSYAYQWFGIT
                     AALVAFFGYSMWRSVSRGRARALPAG"
     gene            complement(10290..11219)
                     /locus_tag="PKV56_11815"
     CDS             complement(10290..11219)
                     /locus_tag="PKV56_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX68544.1"
                     /translation="MPEHFSLTDRARTLHCWCDQYTTRPTDDSHADERTPRCGFARFI
                     VSEVTGEPQQVQTEATQTDPYLMMVVPLEGLFTVRVAERELQLGPGQGCLLSNSVSYA
                     IDFRETFRQLHFCFHVDEISRLYPGWSRKLARAIDCSQGAGAMLKPSLQSALNHGRNV
                     KLAASRSIWRGLQHLMAATLMDSACEQTQPAEPDRSALRTRIDQLIESELRNPELGVS
                     LLTERLGISARHLHRVYAMEQQTLMRTIREARLDRCYLDLTSAELRLMTINEIALSWG
                     FNDQAHFSRHFRLRFGMTAGQARAKPGSASQGG"
     gene            complement(11431..12090)
                     /locus_tag="PKV56_11820"
     CDS             complement(11431..12090)
                     /locus_tag="PKV56_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68545.1"
                     /translation="MDTELPRMNRRSAFLLALLPCIARAQSLPMPSASPIDSVALYVL
                     PTDGISENGAGAIARALTKDTGLWVKSSMWIPPGSIAPLAGTNQYPAEDYLALGGPIS
                     RQLQDANARTYFIVLTDKDINSRTQNFRFQFSMHSPMARTSVLSIARLGYMKDGTPAP
                     PDVVAARVQKMLMRIVGEMRQGWTRTAEPADLMFAPIMSVEDIDRMNLAQSVQKRKQP
                     F"
     gene            12469..13128
                     /locus_tag="PKV56_11825"
     CDS             12469..13128
                     /locus_tag="PKV56_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOX68546.1"
                     /translation="MCTRYITPEQADIERHWLLGRKHLLEWTKAEIFPNRGGPFIRAA
                     REVTEFEREPVIGQWGLIPWFAKSPKLPYSTCNARSEELAGKATFKHPWARGQRCIIP
                     ADSFDEPNWETGRNVWWRFRRSDGLPWGLAGLWNTWVDPTSGELIESYTMLTLNADAH
                     PLMRRMHKPDPKLGPDQQDKRSVVPIEFEDVDTWLFGSATEAAKLIRLSPVEVFDAVP
                     V"
     gene            complement(13191..14537)
                     /locus_tag="PKV56_11830"
     CDS             complement(13191..14537)
                     /locus_tag="PKV56_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX68547.1"
                     /translation="MRWAPDSLLRALACLALSALVYAIAPAPDALRIGLAIFVLIGGL
                     WMTQALHLSVTALLVPLLAVLAGLMDPRTALASFAHPIIFLFLGGFALAAALQRQGLD
                     QVLARGVLGLAAGRRLVAAVLLFALTGLLSMWISNTATAAMMLPLALGLLREQDGPPE
                     RAFVLLGVAYSASIGGIGTLVGSPPNAIAAAQAGIGFAEWLRIGVPLVMLLLPLMAGV
                     LFLVLRPHFGVETPQSPGHSTPQVFTRPQRITLVVFGVTAAGWIGAAPLGRLLGIAAD
                     VDSVIAVAAIVALVASGAIGWPDIEQRTQWGVLLLFGGGLALSEVMAISGASRFMAGA
                     LTSALQGAPPVLILLGVVTFVVFLTELVSNTAAAALLVPIFVGVAVALDLPAPLLAAA
                     IAVSASCAFMLPVGTPPNAIVYATEQVPQATMMRCGLVLNLVCIAVITAVATWAFA"
     gene            14835..>15831
                     /locus_tag="PKV56_11835"
     CDS             14835..>15831
                     /locus_tag="PKV56_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOX68548.1"
                     /translation="MKVGINGMGRIGRLALRAAMGAAERQADDPRAGNRLDVVHLNEI
                     KGGAAATAHLLQFDSVQGRWRADVAAEGERAIRIGERNLSFSAHASAADIPWGDFGVD
                     LVLECTGKFLTPETLQGHLDRGAKRVIVAAPVKVGEVLNIVVGINHHLYEPARHRIVT
                     AASCTTNCLAPVVKVVHEAIGIRHGQITTIHDPTNTNLVVDAPHKDLRRARSAMLSLA
                     PTTTGSATAIALIYPELKGKLNGHAVRAPVLNASLTDCVFELKRETTAEEVNQLFAQA
                     AQGPLAGILGYETRPLVSADYARDTRSSIVDALSTMVTDGTLLKVYAWYDNEMGYA"
BASE COUNT         2949 a         4947 c         4975 g         2960 t
ORIGIN      
        1 ctcgatccag agcgaatgcc cgatcggcct gatcggcgac gacatcgagg cggtgtcgaa
       61 gaagaagtcc aaggaatacg aaggccacac catcgtgccg gtgcgctgcg agggtttccg
      121 cggcgtcagc cagtcgctcg gccaccacct ggccaacgac gccatccgcg actgggtgtt
      181 cgacaagctc gacggcagca agcgcgccga gttcgagagc acgccctacg acgtcaccat
      241 catcggcgac tacaacatcg gcggcgacgc ctggtcgtcg cgcatcctgc tcgaggagat
      301 cggcctgcgc gtgatcgcgc aatggtcggg cgacggcacg atcgccgaac tcgagaacac
      361 gcccaaggcc aagctcaacg tgctgcactg ctaccgctcg atgaactaca tcagccggca
      421 catggaagag aagtacggag tgccttgggt cgaatacaac ttcttcggcc cgaccatgat
      481 cgagaagtcg ctgcgcgaga tcgcgacgca cttcgacgac accatcaagg cgaacgccga
      541 gaaggtcatc gccaagtaca aggccctcac cgatgcggtg atcgccaagt acaagccgcg
      601 cctgcagggc aagaaggtca tgctgttcgt gggtggactg cgtccgcgcc acgtgatcgg
      661 cgcctacgag gacctgggca tggatgtggt cggcaccggc tacgaattcg gccacaacga
      721 cgactaccag cgcacgacgc actacgtcaa ggacggcacg ctgatctatg acgacgtgac
      781 cggctacgag ttcgagaagt tcgtcgagaa ggtccagccg gacctgatcg gctcgggcat
      841 caaggaaaag tacgtgttcc agaagatggg cgtgccgttc cggcagatgc acagctggga
      901 ctactccggc ccgtatcacg gctacgacgg cttcgccatc ttcgcccgcg acatggacat
      961 ggccatcagc agtccggtct gggcgctgac caaggcgcca tggaagaaag ccgcttgatc
     1021 cacctcggct gactggagat gcgggctcgc ctgcatctcc aggcgagtca cctgatttcc
     1081 acttttcggc gtcatcgcca ggagtttcac catgccccag aacgcagaca aggtcattga
     1141 ccacgagatg ctcttccgcg agccggagta tcaagagctg ttcgccaaca agaagaagga
     1201 cttcgagttc aaccactcgg acgaccggat cgccgagatc cgtgactgga ccaagtccaa
     1261 ggaatacaag gacaagaact tcgcccgcga agccttgacc gtgaacccgg ccaaggcctg
     1321 tcagccgctg ggtgctgtct acgtggccaa cggcttccac aagacgatga gcttcgtgca
     1381 cggttcgcag ggctgcgtcg cctactaccg atcgcacttc tcgcgccact tcaaggagcc
     1441 gacgtcgtgc gtgtcctcgt cgatgaccga ggacgcagcc gtgttcggtg gcctgaacaa
     1501 catgatcgac ggcctggcaa acaccttcaa cctgtacaag ccggacatga tcgcggtgtc
     1561 gacgacctgc atggctgaag tcatcggaga cgacctcaac gccttcatca agacatcgaa
     1621 ggagaagggc agcgtgccgg ccgagttcga cgtgccgttc gcgcacacgc cagccttcgt
     1681 cggcagccac gtgaccggct acgacaacgc gctgctcggc atcctgcagc acttctggga
     1741 cggcaaggcc ggcactacac cggcactcga gcgcgtggct gacgaaagca tcaacttcat
     1801 cggtggcttc gacggtttcg tcgtcggcaa catgaaggaa atcaagcgca tcttcgacct
     1861 gttcggctgc gactacaacg tgctgtgcga tccctcggaa acctggaaca cgccgaccga
     1921 tggcgagttc cgcatgtacg aaggaggcac caccaaggaa accgtcatcc gggcgctgaa
     1981 cgccaaggcg acgatcgttt tccaggagta cagctgcgag aagacgatca agtacctgaa
     2041 ggaaaagggc caagaagtcg tggtgctgaa cagcccgatc ggcgtggcag gcaccgacgc
     2101 tttcgtgatg gccctttcca ggttgaccgg caaggacgtg cctgcacaac tcgagaagga
     2161 acgcggccag ctcgtcgatg cgatggccga cagccaggct cacctgcacg gcaagcgtta
     2221 tgcgctgtac ggcgatcccg acatgatgct gggcctgacg cagttcctgc tggaactcgg
     2281 tgccgagccg gcgcacgtgc tggcgaccaa cggcaacgag ggctgggccg ccaaggtgca
     2341 ggcgttgttc gacgcttcgc cttacggcaa gggctgccag gtctatccga agcgcgacct
     2401 ctggcacatg cgctcgctgc tgcacaccga accggttgat ttcctgatcg gcaacaccta
     2461 cggcaagtac ctggagcgcg acaccggcat cccgttgatc cgcatggtgt ttccgatctt
     2521 cgatcgccac cactaccacc gctatccgat ctggggttac gagggggcgc tgcgcaccct
     2581 ggtgatgttc ctggatgagt tcttcgaaac cctcgatgcg aacaccatcg tcccgggcaa
     2641 gacggactac agctacgaca tcattcgctg acggcgttga ctccgtccac ggggcggact
     2701 tccgccccgt ggcgcccagc gccacggggc ttttttgtct tgtcacttct gcgcgaggtt
     2761 cgtcccatga ctgttcccga accggtttat gtcaagcacc tgtccgacga tcgcgtggtc
     2821 gcggtcatcg gccggcacgt atgcatcgac ggacagcccg aggccgatca cctcgtcgtg
     2881 atcgacgagc acccgaaccg gcaggccatc ctgcgcgccg tgccgggcgc cacccacgtc
     2941 gcgggacggg tgccgttgac gatgcctcag gcatcgatgg cgcaggccgc attgcggcgc
     3001 gcgcacgaca gcttcgacgg cagtgctcgg gcggtcaatg aacggctgcg caacgcggtt
     3061 tggcagaagg tgtttgccga cggcgtcgaa tgagttgcaa ccgtcatcga catcaccagg
     3121 cgattccatg atctatgtcg tcgaaatccc ggcacagggt gacgcgcatg cctggttcgc
     3181 gttcaacgag gacgacctgc tgcgcaaggt cgctgcggac gatcggcgcg cagtgcatga
     3241 aatctgggac agcgcgacag cacgtgaact gctggatctc ttcgacgagc agcccgatac
     3301 cgcaggggtg gaagaacgct atcccggcat ccatcggctc ggcatcgagt tcggctggga
     3361 cgcgccgctg ttcagggcgg actacctgca ggagcccggc ctctacgtac ccgaaccgac
     3421 gcgcctgatc gatgcctgcc gcgctgcatt gcgtgcccga ggcggcgaat ggcgcttcta
     3481 tgaaggcgag cagcatgcgc tgaccgccgt cgacaagccg gacgagttgt atgaccggcc
     3541 gggcggctgg cgggcgcgct gggccctgcg tcagcaactg atcgcggtgg aagcccttgc
     3601 cgatgacctc tgaagccggg acgctccagg tcgacgtcgg cgtccccatc gacgccaccg
     3661 acttcgaact cttcgaggtt gcacctaggt ttgttcagga ccgagccgtg ctggatgcca
     3721 ggtggaagtg gcttcagacg caggtgcatc cggaccgctt cgtgacccac ggtgccgtcg
     3781 cccagagagt ggccatgcaa tggacgctgc gcgtcaacga ggcctaccag cgtctgaggg
     3841 ttccgctgtc acgtgccgtc tacctctgcg aattgaacgg ccagtccatc ggagctgaaa
     3901 gcaataccgc gatgcctgcc gagttcctga tgcagcagat ggcctggcga gaggcattgg
     3961 acgaagcgcc gaacctcgag actgtcgagg cacttgccga cgaggttgcc gaacggcagc
     4021 ggaccttgct ggatgatctg gccatcgtga tggacgagct cagcgactgg cctgccgctg
     4081 tggtgcagat ccgcgccctg atgttcgtga cgcgctttgc cgaggacgtg gagcgtcggg
     4141 ctgatgcact gggtggctga gagtgcgcga aggttgttct cgaatcgagc ccccgcgtgt
     4201 ggagcaggct ccgacgctgc cgctgcatgg caggaggcca agtgaccgat gccctcctcg
     4261 ggtccggctt agaagccgaa actcgtaacc aaattcagga taatggcgct gaggatcaat
     4321 ataatgtctc tcacatgcaa gagagatcgg gggggctgga aatatcatcc cccgcggcgc
     4381 cgagaggtgt cacaaaacag tgttgcggct ctctttcgag cggcgacagt caacgcaggc
     4441 gaatgagaca aacaggcccc acaatggtgc ctcggcgaca tgaccatgcg tgattgcgat
     4501 tgcaatccga cttgtcgctc tggacgattg caccgacgca cagccctgag acataggcgc
     4561 catgtgatgc atggcgcagg attactggaa tgcagatgaa gtacttgcgt cgattcggca
     4621 cgttcgctgc cgatgggaag caccggctcc tagccagagg tttggctgtt gctgctcttt
     4681 ggattggcgc atgggccatc gcgccagcgc aggcagccga catccaggga gatgccgctg
     4741 ccggtgcgca gaagaacgcc atgtgcatag gttgtcacgg catcgagaat ttcaagaccg
     4801 gcttcccgat cgtccatcgc gttccgatgt tgcatggcca gaatgcccag tacatcgcgg
     4861 cagcgctggc cgaatacaag gtaggggatc gcaagcatcc gtccatgcgt gccgtggccg
     4921 gaacactcag cgagcaggac atggccgacc tgggtgcgta tttcgagtca gcgggcccgc
     4981 ggaagatcga cccgccagtt gcaacgacca atgatccagc ggtcgcgctg ctcaatcgtg
     5041 gcggatgcca atcctgtcac ggcgccaatt tcaacaagcc gatcaatccc gcctatccca
     5101 agctggccgg gcagcacgcc gactatctct acgttgcgtt gaaggcctac cagcagacgg
     5161 aacccaagcc gttcatcggt cgcggcaatc cgatcatgca atcgatggtc aagcagtttt
     5221 cggcgaacga gttgcagact ctggctgatt acctggagag tctgcccggt gacgttgtga
     5281 ctgtgcagcg cagccgtttt cggtgattga ggggcggcct cggaggccgc cagcacagcg
     5341 gacgacctgg tagtcgaccg cccaccctgt tttgaagaga gaagcgagct atgaacactc
     5401 cgaaaaggat tgcgttcggc gttgcggcgg ccacggcgcc cgcagtttcg atggcggatt
     5461 acagtttcaa ttttcccgat ccggtgacgc ccattgcgca cgagacgctg catgtgcaca
     5521 acctgttcat gatcgtgatc ctggtgatct ttttcgcggt gttcggcatc atgatctatt
     5581 cgatgtgggc gcaccggaag tcgcgcggcc acgcagctgc cggcttcgtc gcacctcggt
     5641 ccggcaagca atggttctgg gtcagcgtcc cgttcgccgc cctgctggtc atcgactacg
     5701 gcgtgttcgg cgtgcctgcg taccacgcca tattgagcta cgaagatacc cgcacggaag
     5761 cggacatggt cgtcaagatc accggcaatc aatggctgtg gcagtacgag tatccgaagg
     5821 agggcatcca gttctccagc cgtttgacca cgcctcgcgc acagatcgac aacgaggagg
     5881 ccaagggcga gcattacctg ctcgaagtcg acaatcctct ggtgctgccg gtcggcaaga
     5941 agatccggtt catcacgacg tctaacgacg tgatccacag ctggtgggtg cctgccttcg
     6001 gcgtcaagcg tgatgcggtg cccggcttcc tgcgggaatt ctgggccaag gtggaccggc
     6061 cgggtaccta ccgagggcaa tgctccgagt tgtgcggcaa ggagcacggt ttcatgccga
     6121 tcgtcgtgca cgtccttccg gaggccgagt tcaatgcctg gctcgctgcg cagaaggcgg
     6181 ggtcggcagc tgctcctgcg gatgctagtg cgccggcccc cgagacgcct gcggcagatg
     6241 cccagaccaa gagctgatac ccttttcccg agaattccag gagtcgcccc atgtccgatg
     6301 caacaacgat tgacgcgcat gaccactaca ccccgggtgg gatgttgcgc tggatatcga
     6361 ccacgaacca caaggacatc gggttgatgt acctgatttt cggtatcacg atgctcgtga
     6421 ccggcggctt gatgatcctc ggggtccgtg ccgagttgtt caagccgggc ttgcaactga
     6481 tgcaacccga attcttcaat cagctgatca cgctgcacgg cctgatcatg gtgttcggtt
     6541 tcgcgatgcc ggtcgccact ggcctggcga actacatgct cccgatgacg ctcggtgctc
     6601 ccgacatggc actgccgcgc ctcaacaact ggggcttctg gattcttcct ccagctgcca
     6661 tcctgttggt tctgccgttc ttccttggaa ttttcggcat tggaccgggt gccgtcgatg
     6721 ccggctggac catgtatgcc ccgctctcta tccagagtgg ctggggtatg gacttcgcta
     6781 tcttcggcat tcacatgctg ggcgtgtcgt ccatcctggg atcgatcaac gtcatcgtga
     6841 cgatcctgaa catgcgcgcg cccggcatga cgctgatgaa gatgccgctg ttcgcccacg
     6901 gcttcctggt caccgcgatc ctgctgatca ccgtcatgcc ggtgctggca gggggcgtga
     6961 ccatgatgct gatggatcgc catttcggaa cccatttctt caacgcagcc ggcggtggtg
     7021 atccggtgct gtggcagcac atcttctggt tcatgggtca ccccgaggtc tacgtgttgt
     7081 ttctgccgat cgccggcgca atccctcacg tcctgaccgc cttcacgcgc aaggaaacct
     7141 acggctaccg tgcgcaggtc tattcgatgt gggccatcgg cgcgctgtcg gtcatcgtct
     7201 gggcgcacca catgttcacc agcggcatgt cgacgaatgc ccagctctac ttcatgtaca
     7261 gcacgatgtt gatctcggtc ccgctggcag tgctgttctt ctgctggatc gcgacgctgt
     7321 ggcgcggttc catgtccttc gaaacgccga tgctgttcgc gatcggttcc atcgtgctgt
     7381 tcggctgggg tgggctgacc gggctcgtgc tcgctgacgc tgccgccgat ccgcagtacc
     7441 acaacgccta cttcctggtg gcgcacttcc actatgcgtt gtatccgaca acggctctgg
     7501 gttcgatggc agccatctac ttctggctgc ccaagtggac cggtcacatg atcaacgagc
     7561 gtctgggaaa attgcatttc tggatcgcga tgatcggttt caatctgacc ttcatcccgc
     7621 aattcatggt gggtctggcc ggcatgccgc gacgcattcc cgactatccg ttgatgttcc
     7681 aagaatggaa catgctgtcc agcattggag cgttcatcct cggcgcgtcg cacctgatct
     7741 tcatctacaa catctaccgc gcctaccaag gcggaccgaa agcggaagcc aagtcctggg
     7801 agggcgcaca gggtcttgaa tggaccctcc cttcgccggc gccttaccac tccttcggaa
     7861 cccaaccggt gatcaagtaa caggtctcgg ggcgagaacg ggcccaggtc gttctcgccc
     7921 cacctgtgga agagatttga agatgaagag tccagtttcc aggagtcctt cgagcggctc
     7981 gcagcccttg agcaatgtgc agctcggatt gatcattggc ggtgtggccg tcgctctgtt
     8041 tttgcttgcg ctgtggaaat ttcggccgtt ttgaacgaga ccgacggtca acggcgcgag
     8101 cgtggaaatc ggcgcctgat cgtcaagctt gccatcgtgg tcgccttctt ttcaggattc
     8161 ggcttcgcga tggttccgct ctacgatgcg ttctgccgaa tcacaggcct gaacggtcgc
     8221 accaatgcgg ccgcgatgcc agtcgttgcc aacacacagg tcgacatggc ccgttcgatc
     8281 cgggtcgaat ttctgagcca ctccatgccg ggagtgggcc tgagtctcaa gcccgagacg
     8341 ttctccattc acgtgaaccc cggcgcgatc atccacacca catacgaagt gcgcaacatc
     8401 actgatcgtg tgtatgtcgg tcaagccgtg ccgagtatca cgccagccac tgccacacag
     8461 tactttcaga aaatcgaatg tttctgcttc gaaaagcaga ccctccagcc cggcgaggtt
     8521 cgcaccatgc cggtggtctt cgtggtccgg ccggaactgc ccgacgcact gcgaaccatc
     8581 acgctgtcgt acacgttttt ccaggccgtt tgagcccgcc cggattccac gattgacctg
     8641 atccccccgc cgaaggaaat caccaccatg tcgcacaccc catcgcccgg gacccacgcc
     8701 cactatttcg tgccggaagt tagtcgctac tccctgcctt tgtccgtttt catcttcctg
     8761 cttgcggcgg gatacgtgct tcgaatcaac gatctcagcg cgggaatctg gggcatgctg
     8821 ctcggtgcag ccgggatcct gttcgtcgtg gtcgcatggt tcggtgtggt catcagtgag
     8881 aacatccgcg gcgtctacac cggctgggag gatcgcacct atcgcatcgg catggtctgg
     8941 ttcatcttct cggaggtgat gttcttcgcc tgcttcttcg gcgcgctcta ttacgttcgc
     9001 ggcatcgcgc tccctgaact cggcagctac gaacctggtc tgacgcccta cagcggcttc
     9061 acaggtgcct ggccgagcag cggcccactg ggggacaagt tcacgccgat gggagcctgg
     9121 ggcattcctg cgctgaacac cgcgttgctc ctgacatcgg gcgcgacgct gacctgggct
     9181 cactggggcc tgctcaaggg caatcgtttc cagttgaacc tgggccttgc gttgacggtt
     9241 gcgctcggtg tggcgttcct gttcttccag gtcgaggagt acatgcatgc ctacagcgaa
     9301 ctcggactga cccttggagc gggtgtgtat ggcgcaacct tcttcatgct gaccggattc
     9361 cacgggtttc acgtgacctt gggcaccatc atgctgatcg tcatgctcgg acgcggcctg
     9421 cgcggccact tctcgaagga caaccacttc gccttcgagg cggtcgcctg gtactggcac
     9481 ttcgttgaca tcgtctggct gctgctgttc gttctggtgt actggctctg aacgtcgccg
     9541 gacggatgct gctcaggcgc tggattgttc cggcactggc cacggttcta ggcgctggcc
     9601 tgttcaccta tctcgggctc tggcaaaagg gcaaggccga gcgggttgag tcgacgattg
     9661 cgcaatacga gcttcgcgct cagcagcctc ccgagatcat cggccccgcg cgaattgatc
     9721 cggcgcaggc tgcaaatgca cgattcacgg tgcgaggccg ctatgagcct gagcatcagt
     9781 tcttcgtcga taaccggcag gaagccgggc aggctggggt gcatgtcgtc acgccgctgc
     9841 ggatcgatca gagcgatgtc cgcatcctga tcaatcgggg ctggatcggc tggccgggag
     9901 gtcgccgggt cctgcccgag gtggcggttc ccggcggcat cgtcgaaatc atcggcacgg
     9961 cgcaggtccc ctcgacccga gagagcctct ggacgcccga tcggcaggag gccgccgcca
    10021 acctctggtc ccgagtcgac ctggcgcgct acgcggcgac gtcgggccat gccgttcagc
    10081 ctgtcgttct gctgcaggac agcggcaacg cgaatgacgg cctcgttcgc cattggccgg
    10141 cacccgagaa ccgggcgccg atgcactgga gctacgccta ccagtggttc ggaatcacag
    10201 cggccctggt tgcgttcttc ggctacagca tgtggcgcag tgtctcgcgc gggcgggcac
    10261 gcgcactacc agccggttga cagggccggt cacccgcctt ggctggcgct gcccggcttg
    10321 gcgcgcgcct gacctgcggt catgccaaag cgcaatcgga agtgccgact gaaatgggcc
    10381 tggtcgttga acccccatga cagcgcaatt tcattgatcg tcatcagccg cagctctgca
    10441 gaggtcaggt ccaggtagca gcggtcgagc cgggcttcac ggatggtgcg catcagcgtc
    10501 tgctgctcca tcgcgtacac ccggtgcagg tggcgcgcag aaatgcccag ccgctccgtg
    10561 agcaggctga cccccagctc cgggttgcgc aattcgcttt cgatcaactg gtcgatgcgg
    10621 gtcctgaggg ccgaacggtc aggttccgca ggctgcgtct gctcgcaagc ggaatccatc
    10681 agcgtcgctg ccatcaggtg ttgcaaccct cgccagatcg agcgggacgc ggccagtttg
    10741 acgttgcgtc catggttcag cgcagactgc agcgacggct tgagcatggc tcccgcgcct
    10801 tgagagcaat cgatcgcgcg agccagtttt cgagaccagc ccggatacag gcggctgatc
    10861 tcgtcgacat gaaagcagaa atgcagctgg cggaacgttt cccggaaatc gatggcatag
    10921 ctgacgctat tgctcagcag gcagccttga ccggggccca gttgcagttc tctttcggcc
    10981 acgcgtacag tgaacagtcc ttccagcggt accaccatca tcagatacgg gtctgtctgc
    11041 gtcgcctcgg tctggacctg ctggggttcg cccgtcactt cggaaacgat gaaccgggca
    11101 aatccgcaac gaggcgtgcg ctcgtcggca tggctgtcgt cggtcggacg cgtggtgtac
    11161 tggtcgcacc agcagtgaag cgtccgagcg cggtcggtca gtgaaaagtg ctcgggcatt
    11221 caggatgaat ttgtcggcga gccagcttgc cgcgcgctcg atcgcggacg cgacggacgc
    11281 atccgtcaac ccaggctgcg cggctttcta gtttatgggc tggacgcgga tcgcctcgca
    11341 gctcgcggac tgccgtcgat gcggtcggtc agactgagga tctgccgcac gctgcgggca
    11401 gcgcggccac acctgcattg acacaacctg tcagaaaggc tgctttcgct tctgaaccga
    11461 ctgtgccagg ttcatgcggt cgatgtcctc gacggacatg atgggcgcaa acatgaggtc
    11521 cgcaggctcg gcggttcgcg tccatccctg ccgcatttcc ccgacgatgc gcatcaacat
    11581 cttctggacc cgagccgcga cgacgtccgg cggtgcgggc gtgccgtctt tcatgtagcc
    11641 gaggcgcgcg atcgagagca ccgatgttcg agccatcggg ctgtgcatgg agaactggaa
    11701 cctgaagttc tgcgtgcgcg agttgatgtc cttgtcggtc aggacgatga agtacgttct
    11761 ggcgttggcg tcctggagct gtctggatat cgggccgccc agagcgagat agtcctcggc
    11821 cggatactga ttggtgcctg ccagcggagc gatgctccca ggcggtatcc acatgctgga
    11881 cttgacccag agccccgtgt ccttcgtgag tgcccgcgcg atggcgccgg cgccgttttc
    11941 cgagatgcca tctgtgggca ggacatacag ggcgacgctg tcgatcggac ttgccgaggg
    12001 catcggtaga gactgagcgc gggcgatgca tgggagcagt gccagcagaa aggccgagcg
    12061 acggttcatg cgtgggagtt cagtgtccat ggctacttgc cagcctttgc atcttctacc
    12121 tggcgcttgt attcgtcata cgaggtgctt gaactcgcca tgcagcgact ccgctgctct
    12181 gtgtcgttga tcctgttgca ctcctggcgc tgccaggcct ggccggcacc atagacctgc
    12241 tgcatcgaac agccctgcac gacgcctgcc agaaggaggt tggccacaag tgcagtacgc
    12301 gcgggcgagc tacgcgagca agtcggggag ttcatctgtg gcttcactgc gcagatctcg
    12361 cggatgaaat cgagttcggt gtggcgtcag gttactcgat gaatgtgact cggcagcgga
    12421 tcgaatcggg aaaccttgcg catccaggcg agcgctacat ttcgccgcat gtgcacacgc
    12481 tacatcacgc ccgaacaggc agacatcgag cgccactggc tgctcgggcg caagcatttg
    12541 ctcgagtgga cgaaggctga gatcttcccc aaccgtgggg gcccattcat ccgcgccgca
    12601 cgtgaagtga cggagttcga acgcgagccg gtaatcgggc agtgggggtt gatcccgtgg
    12661 tttgcgaaat cgccgaagct gccctattcg acctgcaatg cgcggtccga agagctggct
    12721 ggcaaagcca cgttcaagca cccctgggcc cgcggtcagc gatgcatcat ccctgcagac
    12781 tccttcgacg agccgaactg ggagactggc aggaacgtct ggtggcggtt ccggcgttcc
    12841 gacggcctgc cgtgggggct ggccggtctg tggaacacct gggtcgaccc gaccagcggc
    12901 gagctcatcg agagttacac gatgctgacg ctgaacgccg atgcgcatcc gctgatgcgg
    12961 cgcatgcaca agcccgatcc gaagctgggg ccagatcagc aggacaagcg gtcggtggtg
    13021 ccgatcgagt tcgaggatgt tgacacctgg ctgttcgggt cggcgaccga ggctgcgaag
    13081 ctgatcaggc tgtcgccggt tgaggtgttc gacgctgttc cggtctgacc ggcgaccctt
    13141 gctcattgca gcgggggtcg aagccgtggg ccaaggcgcg atccgcttga tcaggcgaag
    13201 gcccaggtcg cgacggcagt gatcactgcg atacagacca ggttcagcac cagaccacag
    13261 cgcatcatgg ttgcttgtgg cacctgctcg gtagcgtaga cgatggcatt cggcggtgtg
    13321 cccacgggca gcatgaacgc gcacgaggcc gacaccgcga tggccgcggc gagaagtggc
    13381 gcaggcaggt ctagcgccac agcgacgccc acaaagatcg gcaccagcag cgccgcggct
    13441 gccgtgttgc tgaccagttc ggtgagaaac acgacgaagg tcaccacgcc cagcaggatc
    13501 agcaccggtg gggcgccttg cagggcgctg gtcagcgcgc cggccatgaa tcgactggcg
    13561 ccgctgatcg ccatcacctc gctcagcgcc aacccgccgc cgaagagcag cagcacaccc
    13621 cactgcgtcc gctgttcgat gtcgggccag ccgatcgcac cgctggccac cagtgcgacg
    13681 atggccgcca cggcgatgac actgtcgaca tcggccgcga tgcccagcag cctgcccagc
    13741 ggcgctgcgc cgatccatcc ggcggcggtg acgccgaaca cgaccagggt gatgcgctgg
    13801 gggcgtgtga agacctgcgg cgtggagtgc ccgggcgact gtggcgtttc gacaccgaag
    13861 tgggggcgca gcacgaggaa gagcacacca gccatcagcg gcagcaacag catcaccagt
    13921 ggcacgccga tgcgcagcca ttcggcgaag ccgatgccag cctgcgccgc cgcgatggcg
    13981 ttgggcgggc tgcccaccag cgtgccgatg ccgccgatgc tggcgctgta ggccacgccc
    14041 agcagcacga aggcacgctc cggcggtccg tcttgttcgc gcagcaggcc cagtgccagc
    14101 ggcagcatca tggcggccgt cgcggtgttg ctgatccaca tcgacagcag ccccgtcagt
    14161 gcgaacagca gtaccgcggc gacgaggcgc cggcctgcag ccaggcccag cacgccccgg
    14221 gcgagaacct ggtccaggcc ctgccgctga agggccgcgg ccagcgcgaa gcccccgagg
    14281 aacaggaaga tgatggggtg cgcgaacgac gccagcgccg tgcgcgggtc catcagcccg
    14341 gccagcacgg ccagcaacgg aaccagcagc gcagtgacgc tcaggtgcag cgcctgcgtc
    14401 atccacaggc cgccgatcag cacgaagatc gccaggccga tgcgcagggc gtcaggtgct
    14461 ggtgcgatcg catacaccag cgctgacagc gccaggcatg ccagcgcgcg caacagcgaa
    14521 tcaggggccc acctcatgca tcgctcccgg tggagggctg cacatcggtg ctgacatcgg
    14581 ccggcacgtg gcagctcgag tgattcaagg tgaggtctcc tgttcgaggg tgtcgcgcgt
    14641 gggacctgtc atcccatgct cgtccgcatc ctgaaatgga tcgagccaag ggtggattct
    14701 cgatgtttcc ccatgccctc cggcccggca gtcgggtagc gcggctgtcg ccgaaccgtc
    14761 acaaacaatt cttatatttc aatgttgttg gaatcattaa atagttctct cgaactcgat
    14821 acgggatcac aagcatgaag gtcggcatca acgggatggg ccgcatcggg cgattggctt
    14881 tgcgcgcagc gatgggagca gccgagcggc aagcggatga tccgcgcgca ggcaatcggc
    14941 tggacgtggt ccacctcaac gagatcaagg gcggggccgc ggccacggcg catctgctgc
    15001 agttcgacag cgtgcaggga cgctggcgtg ccgatgtggc cgccgagggt gagcgtgcca
    15061 tccgcatcgg cgagcgcaac ctgtcgttct cggcgcacgc cagtgcagcc gacatcccct
    15121 ggggcgactt cggggtcgac ctggtgctgg agtgcaccgg caagttcctg acacccgaaa
    15181 ccctgcaggg ccacctggac cgcggcgcca aacgggtgat cgtcgcggcg cccgtgaagg
    15241 tgggcgaggt gctcaacatc gtggtgggca tcaatcacca cctgtacgag ccggcgcggc
    15301 accgcatcgt caccgcggcg tcctgcacca ccaattgcct agccccggtg gtgaaggtgg
    15361 tgcacgaggc catcggcatc cgccacggcc agatcaccac catccacgac ccgaccaaca
    15421 ccaacctcgt ggtcgacgcg cctcacaagg atctgcgccg cgcccgcagc gccatgctca
    15481 gcctggcgcc gacgaccacc ggcagcgcca cggcgatcgc gctgatctac cccgagctga
    15541 agggcaagct caacggccat gcggtgcgcg cgccggtgct caatgcatcg ctcaccgatt
    15601 gcgtgttcga gctgaagcgc gagaccactg ccgaggaggt gaaccagttg ttcgcgcaag
    15661 ccgcccaagg cccgctggcc ggcatcctcg gttacgaaac ccgtccgctg gtcagcgccg
    15721 attacgcccg cgacacgcgc agcagcatcg tcgatgctct gtccacgatg gtcactgacg
    15781 gcacgctgct gaaggtctat gcctggtacg acaacgagat gggttacgcc t
//
LOCUS       DAOORV010000084        15585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4613_length_15585_cov_7.280489, whole genome shotgun sequence.
ACCESSION   DAOORV010000084 DAOORV010000000
VERSION     DAOORV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_15585_cov_7.280489"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PKV56_11840"
     CDS             complement(<1..1372)
                     /locus_tag="PKV56_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HOX68549.1"
                     /translation="MLGMSKANGADTRPGRSGFRPRHWAQAQAENPLARFIPFSSLVS
                     PHDVITRGGDYLRVWRLDGVAFECADDHQIGERHEALCSLMRNLAGGQWAVWTHRLHR
                     VVSDNLQDPAQPGFARDLSQAYQAKLGERRMMSNELYLTLVYRPNVSRVSRALQSTQR
                     TRAAIAEAQAEALRVMEERTALVGRVLRGFGPELLGTRERRGRTYSELAEFLGYLVNG
                     CWRPIPMTAGPLYRTLPTARLSFGGDKLELRQGDQRRYAALVDIKEYADAVEPGILNA
                     LLYEASEFIETQSFSILPRREATRALELQRDQLIASDDVVASQVAEMDVALNELGDGQ
                     FCMGEYHYSLVVFGEDVADAGRRAAQAIGAVGEASSLQMSPVDLVADAAWFAQMPGNW
                     QWRPRDAKLSSRAFAALSSGHNFARGKRDGNPWGEALALLRTPSGQPFYLNLHSSPEG
                     EDSADKK"
     gene            complement(1356..1688)
                     /locus_tag="PKV56_11845"
     CDS             complement(1356..1688)
                     /locus_tag="PKV56_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HOX68550.1"
                     /translation="MQGEAIYKGATRPAMKLGIPLVPLVVLFGTGMLLIMWGGTLVSW
                     WIALGVVVAFVPALLWMRFVTSKDDQRFRQMFVAMKLRLHDRNRRFWHARSYAPTLYR
                     GARDAWHV"
     gene            complement(1704..1994)
                     /locus_tag="PKV56_11850"
     CDS             complement(1704..1994)
                     /locus_tag="PKV56_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HOX68551.1"
                     /translation="MTTLASIRRAAPGLAALAAALLSQAVHAQGFDKINTTVTNVNTI
                     LVTISIAVVTIAIIWAGFKMIFQGARLADVANVLIGGTLVGGAAAFASYIVT"
     gene            complement(1991..2485)
                     /locus_tag="PKV56_11855"
     CDS             complement(1991..2485)
                     /locus_tag="PKV56_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOX68552.1"
                     /translation="MDASIFLALALACAPQVHADTARALVSVESAFNPWAIGVVGGAL
                     MRQPRHRAEALATAKALREAGWHFSVGLGQINVGNFDRLGLTVEAAFEPCTNLAAMQT
                     VLAECFDRASGSAANAVDQVALRQALSCYYSGNFATGFLLGYVRKVAIAARAVPTAQP
                     KEKV"
     gene            complement(2497..3054)
                     /locus_tag="PKV56_11860"
     CDS             complement(2497..3054)
                     /locus_tag="PKV56_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68553.1"
                     /translation="MDTNRVPGWTLLRATRPRLLSVPLLLLATLLGLSRPASALDGCM
                     VMLCLAAPSWREIPMCVPTMHDLNKHLARGRRFPTCADAGAGNTAGNEWASAPDFCPP
                     QYTRRIDGESGSTYTCDFMGAVTVNVNGQPFTRTWWNAAGDTVTEFSAAAKAQLGTWD
                     TKFDDDYAAWFAALPPPAPVVESGY"
     gene            complement(3317..4090)
                     /locus_tag="PKV56_11865"
     CDS             complement(3317..4090)
                     /locus_tag="PKV56_11865"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX68554.1"
                     /translation="MTRSETAPFMPDRPSTNAAMVFAAAERLGSAPEPASVLALLKAG
                     ADALGAEGAVFVSVARDAAINPATRLMTTCDPVWLQHYLDAGCLVEDPWFRYASHRSE
                     PIVASELDTRNASTSAHDTLAMHRSFASAVLAPAHSGLGHDRVGLLCLGSSQRGYFET
                     GQPVSVRIAARLLAAGLSDWWLANARRELLVTARLTPAEMELLRHQLLGHSSKRIAAS
                     LNVSHSSINSRFQRMNMKLGVPNRRAAVRLALECGLLFG"
     gene            complement(4436..4969)
                     /locus_tag="PKV56_11870"
     CDS             complement(4436..4969)
                     /locus_tag="PKV56_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68555.1"
                     /translation="MNYRSWCALAIGSMVIACTAQPIRPIASKAVVAVPSSANCTFDA
                     THRQSQNCCVMQAYRARVDVDVAYDTVVREYGFRPEPREYELEGEAYPDRFHGHRHET
                     HAGHSYRLKGIVVPRSDARLARGLWLGLDLRGVEPGSVEVAPLYCSVGGWSMENQALW
                     HDVVQRSIRSTLPAKVD"
     gene            complement(5015..6589)
                     /locus_tag="PKV56_11875"
     CDS             complement(5015..6589)
                     /locus_tag="PKV56_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX68556.1"
                     /translation="MAKSTRSAEAASHPGSRPESSQTRPPLNADAARRSAIPNGTNSR
                     FTEAEIAEAAELMAAEHRGKRLLMSVTKGHRTLAMRHGTAAVVVGALTVAALYVIELK
                     SPSVDAGTFRAWLLFSVASIFGRLAMYIWLFSAYSPVQVVKSTPLRLVPLAIASISAA
                     QWIWSIDLFASDRASAASFVLFAGLVGNSVAVAGMWPTAPLAVLAYLTATWATLFHSL
                     YTASPDADAIIVASAACVGVVLWASSYLQVHQVKNILNRSDEVDLLLARLHRANAELT
                     AANSTLDAMNSAATTELEHRSMFFTSASHDFRQRLHAMNLLANAALGSSCEDGNESAP
                     LRRLADCIEDVEHYVTHVLDFARFDRSATRPVRQRVELQSLFQKLELNFEDIATERGA
                     TLVIRSTDVVLFTDAAMLQRILENLISNAIKFTSKRVLVAARKRGANIAIEVWDQGDG
                     IAPEDRAAIFTPFYRSPTAERHHGGVGLGLAVVQRFAECLGCEIQLRSQMGHGTVMTL
                     LLPSGVVPDTRRPVGH"
     gene            complement(6614..7300)
                     /locus_tag="PKV56_11880"
     CDS             complement(6614..7300)
                     /locus_tag="PKV56_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX68557.1"
                     /translation="MAQAVLLVEDHPLVAEAMLGALRRSRSGLDIHVAPDAPTTLRYL
                     REAEQNWFRVFLDLDVPGAHGLSLARDVERAHLADRCCVVSASDREDLIGEVRLLGFL
                     GYIVKATPIADFKRCVAEVLGGARTFPRVALDTRSPIRLTRRQEQVLDCVRRGLTSKE
                     IARVVLLSEGTVNNCINAALKALHVTSRSHAVAKALELGLLKVDVRSPETQAASSVDG
                     SGALCSIPAR"
     gene            8006..9940
                     /locus_tag="PKV56_11885"
     CDS             8006..9940
                     /locus_tag="PKV56_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX68558.1"
                     /translation="MEHTKELLSVPLASLVPSRFNVRRHSVGQVEELAALIEAQGLLH
                     NLVVTEQEVGRGKSRKLKFAVAAGERRRRAMLLLQQRGRLPKEHEVLCELVPPERALE
                     VSLAENSGREAMHPADEFEAFKALIDEGKGVEDVAARFGVSVLTVQRRLKLSALSPKL
                     LALYREDGINLDQLMALTLSDDHAVQERTWFDAQPWDKTPAALRRRLTVGEVEAAGSA
                     LVRFVGIEAYEAAGGVVRRDLFDDAQSRFLSDPALLERLATEKLETLAGTVREEGWKW
                     VEARLSVDSMALRQFAPCAHASRKPTAAEQEELDALERRAAELEQQAQALDDAPEWSP
                     DEAEMIDLEEQDIAARRKVIHEALKTWTPEQKTHAGVIVTIGRDGDVEVMRGLVRDED
                     RKAIAAAVRAKGAKEHRDPGDAGDDEGTPSMSMAPECRTPGCSDALTKRLAAHRTMAL
                     QAMLAQNTAVALASVVHVFVLRAFGADYPREASALQVTPQLSAFALEAMADDLKTSRA
                     WRAVQHAKEVWRARLPEDQGEWLGWLINLPQAELIDLLALCGALTVNALPGAGATGSA
                     NAIAAALGLDMADWWEPTAEAYLNHVPKAQIIAALKEAGPELAGGGVEAMKKDALVSA
                     AASRLAGTRWLPEPLRRSPG"
     gene            9994..10299
                     /locus_tag="PKV56_11890"
     CDS             9994..10299
                     /locus_tag="PKV56_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68559.1"
                     /translation="MWLLVAREPRPDAPDWPGRRLLAAIDAVAWPLMWVLLIRQVPGP
                     AGLVGPFVTALAVLLGLGRLHRALWVNHRYWFTTWRWGKVLGAMLLIGTVMKLSMSA"
     gene            complement(10338..10568)
                     /locus_tag="PKV56_11895"
     CDS             complement(10338..10568)
                     /locus_tag="PKV56_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOX68560.1"
                     /translation="MSQTQTESKAPAAEVQPAVFVRMAAVVRMTGLGRSTIYRLMAED
                     KFPSPVRLAKRAVAWRRIDLEQWSAGRPTVSH"
     gene            complement(11172..12410)
                     /locus_tag="PKV56_11900"
     CDS             complement(11172..12410)
                     /locus_tag="PKV56_11900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX68561.1"
                     /translation="MTDLKKAIDTVLDGVTTGTPRVPGVSAAVTDRRGTIYEGARGVR
                     NTGSGEPFQPDTVCAIFSTTKAIAGTACLQLVEEGLLDLDAPAKEYVSQLGDIMVIEG
                     FGADGKPKLRPPRRDVTTRHLLTHTAGFTYDFFNETYTRLANEQGQPWVVDASWKSIK
                     TPLLFDPGERWEYGSNIDWAGMVVEGITGKRLGDVMRSRILNPLGMTDTAFTMTPSMQ
                     SRQATIHAREDRDGSLIPLNGVTLPQDPEVHMGGHGLYGTASDYVKFIRMWLNDGRSD
                     SGEQILRPETVEMASRNHLPKGMHIKMLPGVGATSTAASILPVSKPRLSNDAEFFPGM
                     PKTWALTFMINEEAAPTGRPAGALAWAGLANLYYWIDRANGVGGYWATQIFPFADPTS
                     VAGFLAMEKATYQHATLRAR"
     gene            complement(12610..13644)
                     /locus_tag="PKV56_11905"
     CDS             complement(12610..13644)
                     /locus_tag="PKV56_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX68562.1"
                     /translation="MQELLARLKALDPNASLALRIIACFDELVRGGVNIHGLLGAAAS
                     LSGCVAGYAGHESARTLRVSPGGEVLKDGGVEKRVGALEADGMTVWLERDGPARVNDR
                     MILERLALALAIRSGHTAEDPLRRLGDLLDANVDVEQRRAAAAKLGLAPRQKYRVATL
                     PLFATWNQHRELLEDVVHTRFGPLHVCVLRADVKQIDASPCGIGVATGVDDLHRSFAT
                     ALVSLRLCAIPEVPVVVADDYGGLVDLLAQCTIDRPLLDVEELESVMQHPWAAQTLDA
                     LLRSASIRDASRIAGVHHSTMQARLETIRGLLSFDPMDGIGRTRVGLAFLAWRVRHST
                     ALDLPPPTGR"
     gene            complement(13652..14656)
                     /locus_tag="PKV56_11910"
     CDS             complement(13652..14656)
                     /locus_tag="PKV56_11910"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOX68563.1"
                     /translation="MNERTLGSIEAVDRPARRDRPAFDPELRAGLAIVGGMFPPTITS
                     DLIDFMRVSYAGPSVDTLIGGRSICCEERVFEGYEGHALKASVFRHERASGLRPGVLF
                     AHSGGLMFGDRFSGLDRMIDFVELLGVVLVSIEYRLAPEHRDPYPREDVYAALEWMAS
                     HAESLGVRLDRLLIAGASAGGGLAAGVALAARDRGGPRLCGQLLDYPMLDDRGLTKST
                     RQFDGIGVWDRVSNETGWSALLGDARGGPDVSVYAAPARATQLHDLPPAFLDVGSAEI
                     FRDETVAYADALWNAGGDAELHVWPGGFHAFDIFAPHTALSRGMIATRSAWMSRVLGD
                     "
     gene            15009..15551
                     /locus_tag="PKV56_11915"
     CDS             15009..15551
                     /locus_tag="PKV56_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68564.1"
                     /translation="MMRLKHSLGLAPHTQLASQAQPIAMFRHRRQLRQWAARVLLLWL
                     FGIAAGVANACITTEQATPVAAAASHLAEAAEAHNDAVPHDHAQADRLALPSHHADDS
                     THIANLSKANCQDFCDKATVSIPPVKSALDDGQSHAVIAMTAMTVVPMPAFAPVQLRV
                     PRRDGVLAPPIPIAFLRLAL"
BASE COUNT         2565 a         5101 c         5401 g         2518 t
ORIGIN      
        1 gcttcttgtc ggcggagtct tcgccttccg ggctgctgtg caggttcagg tagaaaggct
       61 ggcccgaggg cgtgcgcagc agggccagcg cctcacccca ggggttgcca tcgcgcttgc
      121 cacgggcgaa gttgtgcccg ctggacagcg ccgcgaaggc gcgcgagctg agcttggcat
      181 cccggggccg ccattgccag ttgcccggca tctgcgcgaa ccaggcggca tcggccacca
      241 ggtcgactgg ggacatctgc aggctggagg cctcgccgac ggcgccgatg gcctgcgccg
      301 cgcggcggcc tgcatcggcc acgtcctcgc cgaatacgac caagctgtag tggtactcgc
      361 ccatgcagaa ctggccgtcg ccgagttcgt ttagggccac gtccatctcg gcaacctggc
      421 tggccaccac gtcgtcactg gcaatcagct ggtcgcgctg cagttcgagg gctcgcgtcg
      481 cctcgcggcg cggcaggatc gagaagctct gcgtctcgat gaactcgctg gcctcgtaga
      541 ggagcgcgtt gaggatgccg ggctcgacgg cgtcggcgta ctccttgatg tcgaccaggg
      601 ccgcgtagcg gcgttggtcg ccttggcgaa gttcgagctt gtcgccaccg aaggacaggc
      661 gcgcggtggg aagcgtccgg tacagcgggc ccgcggtcat cgggatcggc cgccagcagc
      721 cgttgaccag gtagccgagg aactcggcca gctcggagta ggtccggccg cggcgctcgc
      781 gcgtgcccag cagttcgggg ccaaagccgc gcagcacgcg gccgacgagt gcggtgcgtt
      841 cctccatcac gcgcagggcc tcggcctggg cctccgcgat ggcggcgcgc gtgcgttggg
      901 tcgattgcag ggcgcggctc acccgggaca cgttgggccg gtagaccagc gtcaggtaga
      961 gctcgttgct catcatccgg cgctcgccaa gcttggcctg gtaggcctgg gacaggtcac
     1021 gtgcgaagcc gggctgcgct ggatcctgga ggttgtcgct gacgacgcgg tgaaggcggt
     1081 gcgtccagac ggcccactgg ccgccggcca ggttgcgcat caggctgcac agggcctcat
     1141 gccgctcgcc gatctgatga tcgtcggcgc actcgaaggc cacgccgtcg agccgccaga
     1201 cgcgcaggta gtccccgccg cgcgtgatca cgtcgtgcgg gctgaccagc gacgagaacg
     1261 ggatgaagcg ggccagcggg ttctccgcct gggcctgcgc ccagtgccgg ggcctgaacc
     1321 cactgcggcc cggccgggtg tcggcaccgt tggccttaga catgccaagc atcgcgggct
     1381 ccccggtaga gggtgggcgc gtagctgcgc gcatgccaga agcggcggtt gcggtcgtgc
     1441 aggcgcagct tcatggcgac gaacatctgg cggaagcgct ggtcgtcctt ggaggtgacg
     1501 aagcgcatcc acagcagcgc gggcacgaag gccacgacca cgccgagggc gatccaccag
     1561 ctcaccaggg tgccgcccca catgatcagc agcatgccgg tgccgaacag caccaccagc
     1621 ggcacgagcg ggatgcccag cttcatggcc gggcgtgtcg cgcccttgta gatggcttcg
     1681 ccttgcatgg tcgggcccgc cggtcaggtc acgatgtagc tggcgaaggc ggcggcgccg
     1741 ccgaccaggg tgccgccgat gagcacgttg gcgacatcag ccaggcgggc accctgaaag
     1801 atcatcttga agccggccca gatgatcgcg atcgtgacca ccgcgatcga gatcgtcacc
     1861 aggatggtgt tgacgttcgt gaccgtggtg ttgatcttgt cgaagccttg cgcgtggacc
     1921 gcctgcgaga gcagggccgc cgccagcgcg gccaggcccg gcgcggcgcg ccggatcgat
     1981 gcgagggttg tcataccttc tccttcggtt gggcggtggg aacggcacgt gcggcgattg
     2041 caaccttgcg cacgtagccg aggaggaagc cggtggcgaa attgccgctg tagtagcagg
     2101 acagggcctg gcgcagggcg acctgatcca ccgcgttcgc ggctgatccg ctcgcgcggt
     2161 cgaagcactc ggcgagcacg gtctgcatgg ccgccaggtt cgtgcatggc tcgaaggcgg
     2221 cttcgacggt caggccgagc cgatcgaaat tgccgacgtt gatctgcccg aggccgacac
     2281 tgaagtgcca gccggcttcc cgcagggcct tggcagttgc cagcgcctcg gcgcggtgcc
     2341 gtggctggcg cataagcgca ccaccgacca cgccgatcgc ccacgggttg aaggcgctct
     2401 cgacgctgac cagggcccgt gccgtgtcgg cgtgcacctg gggtgcacag gcaagcgcca
     2461 gcgcgaggaa gatggacgca tccatggccc ggtgcctcag tagccggact cgacgacagg
     2521 tgcgggcggc ggcagcgcgg cgaaccacgc ggcgtagtcg tcgtcgaact tggtgtccca
     2581 ggtgcccagc tgggccttcg ctgcggcgct gaactctgtc accgtgtcgc ccgccgcgtt
     2641 ccaccaagtg cgggtgaacg gctggccatt gacgttgacc gtgactgcgc ccatgaagtc
     2701 gcacgtgtac gtcgagccgc tctcgccgtc gatccgtcgt gtgtactgcg gcgggcagaa
     2761 gtcaggggcg ctcgcccact cgttgcccgc ggtgttgccg gcaccggcat ctgcgcaggt
     2821 cgggaaccgc cggccgcgag ccaggtgctt gttcaggtcg tgcatggtgg gaacgcacat
     2881 ggggatctcg cgccagctcg gggcggcgag acacagcatc accatgcagc catcgagagc
     2941 agaagcaggc cggctgagac cgagcaacgt ggcgaggagc aggagaggga cgctcagcag
     3001 tcgagggcgc gtggcccgca acagagtcca gcccggtact cggttcgtgt ccatgttgat
     3061 ccccaaggtg aagcaacgga gaagtcagct cgggatcgag tgcgcccgtc ccttgagatg
     3121 aatctaggcg accgactcgc aggctcatgc gatgactttc agccgcgaat catcgaagtc
     3181 cgataggacg gccgtttgct ccgagccaag ccgcggccac ttgccgagag gtgtcaggcg
     3241 cctcgacagc acaacgtcaa cgcgcggtgt gcagccggcg ttcgcacccg gcgtgcgtcg
     3301 acggctgtct aggctgctac ccaaacaaca agccgcactc cagggccagg cgcacagcgg
     3361 cacgtcggtt gggaacgccc agcttcatgt tcatgcgctg gaagcgcgag ttgatcgaac
     3421 tgtggctgac gttcaaactg gcggcgatgc gtttgctgga gtgccccaga agttgatgcc
     3481 tcagcagctc catctcggca ggcgtgagcc gcgccgtgac aagcaactcg cggcgtgcgt
     3541 tcgccagcca ccagtcactt agtccagcgg caaggagccg ggcagcgatc ctgacgctca
     3601 ccggttggcc cgtctcgaag taccctcgtt gcgaggagcc caggcacagc aggcccacac
     3661 gatcgtgccc caggcccgaa tgggccggtg ccagtaccgc tgacgcgaag ctccggtgca
     3721 ttgccagagt gtcgtgcgct gacgtgctgg cgttgcgcgt gtccagttcg ctggccacga
     3781 tcggctccga tcggtgtgac gcgtagcgaa accagggatc ctccacgagg cagccggcgt
     3841 caaggtagtg ttgaagccag acagggtcgc aggtcgtcat caggcgggtc gccggattga
     3901 tggcggcatc gcgggcgacg ctcacgaaga ctgcgccctc ggcccccagg gcgtccgcgc
     3961 ccgccttcag cagcgccaga accgatgccg gctccggtgc cgatccgagt cgctcggcag
     4021 cggcaaacac catcgccgcg ttggtcgacg gcctgtctgg catgaatggc gcggtctcac
     4081 tgcgggtcat cgcggcaagg aagcaaccac aggcggtccg cctcgcgcct gaggtcgatg
     4141 tgggcgatcg ggtgggcgaa ccgtacggga tcggagtcgt gccagcgttc gaatgcatcc
     4201 gcgcagtcga acagcatgga aatcatggtt cgtggcccgc gcccggggat ctcctcgcgt
     4261 tcgacgtaca gaccgctcga cacccgaacg aggacgtatc gaatcgccgc acgctcccct
     4321 ttcgcgacaa gtctgaacgt gcgcggaccc aggtccccag gcgagccgaa ctctgcatcg
     4381 cgaggcgcag gctcaggggc gctcgcgcgc cgggctggtt gagtgccggc catggtcagt
     4441 cgaccttcgc gggaagggtg gagcggatcg atcgctgaac cacgtcgtgc cagagtgctt
     4501 ggttctccat ggaccagccg ccgaccgaac agtagagggg agccacctcg accgaaccag
     4561 gctcgacgcc tcgcaggtca aggcccagcc acagccctcg ggcgagccga gcatcgctcc
     4621 gcggcaccac gatgcctttc agccggtacg aatgtccggc atgcgtttca tgccggtgtc
     4681 cgtggaatcg atccgggtag gcttcaccct caagctcgta ttcccgcggt tcgggtcgga
     4741 agccgtactc ccggacaacc gtgtcgtacg caacgtccac gtccacgcgg gcccgatagg
     4801 cttgcatgac gcagcagttc tggctttgcc ggtgggtcgc atcgaaggtg cagttcgcac
     4861 tgcttggaac cgcaacgacc gccttgctgg cgatcgggcg aatcggctgg gcagtacacg
     4921 cgatgaccat cgagccgatg gccaacgcac accaggatcg atagttcatg ttcgcctccg
     4981 ttcaagggtt gcggtcgcgc cgatgtcgcg cgaactagtg acccaccggt cgtcttgtgt
     5041 ctgggacgac gccgctcgga agaagcaagg tcatcacggt accgtggccc atctgcgatc
     5101 gcagctggat ctcgcaaccc aggcactccg cgaaacgctg gacgaccgcg agtccgaggc
     5161 cgacgccacc atgatgccgt tccgccgtgg gcgacctgta gaacggcgtg aagatggcgg
     5221 cccggtcttc gggcgcgatg ccgtcacctt gatcccacac ctcgatcgcg atgttcgcgc
     5281 cgcgcttgcg ggcagccacc agcacgcgct tcgatgtgaa cttgatcgcg tttgagatca
     5341 ggttctccag gatgcgctgc aacatcgcgg catcggtgaa cagcacaaca tcggttgacc
     5401 ggatcacgag cgtggcgccg cgttccgtcg cgatgtcctc gaagttcagt tcaagtttct
     5461 ggaagagcga ttgcaactcg acccgttgtc gcaccggccg ggtcgcactg cggtcgaacc
     5521 gggcgaagtc cagcacatgc gtcacgtagt gctcgacgtc ttcgatgcag tccgcaagtc
     5581 gcctcagggg tgctgactcg ttgccgtcct cgcagcttga accgagtgcg gcgttggcca
     5641 gcaggttcat ggcgtgcagg cgctgccgga agtcgtggct tgcgctggtg aagaacatcg
     5701 accggtgctc gagttccgtc gtggcggcgc tgttcatagc atccagtgtt gagttggccg
     5761 cagtcagctc ggcgttggcg cggtgcagcc gcgccagcaa gaggtcgact tcgtcgctcc
     5821 tgttgaggat gttcttcacc tggtggacct gcaggtagga gctggcccag aggacgaccc
     5881 caacgcaggc cgccgacgca acgatgatcg cgtccgcatc cggtgaggcg gtgtagaggc
     5941 tatggaagag agtggcccag gtcgccgtca ggtaggccag cacggcaagc ggagcggtag
     6001 gccacatgcc ggctaccgca acgctgttgc ccaccaggcc ggcgaagagg acgaacgagg
     6061 ccgcactggc gcggtccgaa gcgaatagat cgatcgacca gatccactgc gctgcggaga
     6121 tcgacgcgat ggccagcgga acgagccgca gcggcgtcga cttcacgacc tgcaccggcg
     6181 agtaggctga aaagagccag atgtacatcg cgagtcgacc aaagatgctg gcaacgctga
     6241 acagcagcca ggctcgaaac gtgccagcgt cgactgacgg cgacttcagt tcgatgacgt
     6301 acagcgccgc cacggtcaaa gcgccgacga cgaccgccgc ggtgccgtgt cgcatggcga
     6361 gcgttcgatg acccttggtc acgctcatca gcagacgctt gccgcggtgc tccgccgcca
     6421 tcagttcggc cgcttcggcg atctcggctt cggtgaagcg ggagttcgtt ccgttcggga
     6481 tcgcgctccg cctcgctgcg tccgcgttca ggggcggccg agtctggctc gactcggggc
     6541 gactgccagg gtggcttgct gcctctgcgc tacgtgtgga cttggccatc tcacgctcac
     6601 tgggcctgtg gcttcagcgc gccgggatgc tgcacagagc gccggacccg tccacactgg
     6661 aagctgcctg ggtctcgggc gaacgtacgt cgaccttgag cagtcccagt tcgagcgcct
     6721 tggcgacagc gtgactgcgg ctggtgacgt gcagggcttt caaggccgcg ttgatgcagt
     6781 tgttgacggt accttccgac aggagcacaa cgcgagcgat ctccttggag gtcaggccgc
     6841 ggcggacgca gtccagcacc tgctcctggc gtcgagtgag ccggatcggc gagcgcgtgt
     6901 ccaatgccac gcggggaaag gtacgcgcgc cgcccagcac ttcggccacg cagcgcttga
     6961 agtcggcgat cggcgtcgct ttcacgatgt acccgagaaa gccgagcagt ctcacttccc
     7021 cgatgaggtc ctcacgatcc gacgcgctca ctacgcagca ccggtcggcc aggtgcgcac
     7081 gctccacatc gcgggcgagc gacagaccgt gtgcaccggg tacgtcgagg tccaggaaga
     7141 cgcggaacca gttctgctcg gcctcgcgca ggtagcgcaa ggttgtcggt gcgtccgggg
     7201 ccacgtgaat gtccaacccc gagcgtgacc gacgcaacgc gcccagcatc gcttctgcga
     7261 ccagaggatg gtcctctacg agcaagacgg cctgcgccat ggtcctcaaa gcttggaccc
     7321 gatgcggttg ctggtcaaca tgcataacta accgactttg tatccagccg gcgtcgatcg
     7381 cagtcgtggg ccgcgccgtc tcgacgaggt cacccgggcc ggctcgccag cgatgctgcc
     7441 gagctcttcg tggctgatag gggggccatt tcccctgcgt gtccccctag gcagtcctta
     7501 gagtgaacgc cacttcttga ccgttctttg agtagccgcc cccagcgctc ggttgtcggc
     7561 acccgccctc gttggtcgcc tacgttgcga ccgtagcgcc gcgcgcgccg tcgcaagggc
     7621 ctttcgcggc ataggccggt gccctcccgc ctgcggcggt tccctcccag ctattccacg
     7681 gtgcccttgc gcccgcgctt gttcgcggcg ctgtcggtgt cgcacggcga ccgacgaatg
     7741 cggggcagca gtccgctcaa cccaacgccg agggatcaac agccaatccg gctccctcgg
     7801 aaattagggc aggcggtttt cagacaagaa gggcagacga ggtcttcatg cgagtcgaga
     7861 ttcgagtcag cgagtgggca cggcgccgtt tacgaaacgt tgccgtgtcg ttcgaaacct
     7921 gggcctttcc ctggcccggc gtgccgccgt aatgcgttgt cgaaggacgg tcgcagagtg
     7981 tcatcaacca atggagttca tcatcatgga acacacgaag gagttgctct cggtgccgct
     8041 ggccagcctg gtgccgtcgc ggttcaacgt ccgccgtcac tcggtcgggc aggtcgagga
     8101 actggcggcg ctgatcgagg cgcagggcct gctgcacaac ctggtggtca ccgagcagga
     8161 ggtcggccgg ggcaagtcgc gcaagctgaa gttcgcggtc gcggccggcg agcggcggcg
     8221 ccgcgcaatg ctgctgctgc agcagcgcgg gcgcttgccg aaggaacacg aggtgctgtg
     8281 cgaactggtg ccgcccgagc gtgcgctgga ggtcagcctc gccgagaaca gcgggcgcga
     8341 ggccatgcac ccggccgacg agttcgaggc cttcaaggcg ctgatcgacg agggcaaggg
     8401 cgtcgaggac gtggcagcgc gcttcggcgt gtcggtgctg acggtgcagc gccgcctgaa
     8461 gttgtcggcg ctgtcgccga agctgctggc gctgtatcgc gaggacggca tcaacctgga
     8521 ccagctgatg gcgctcacgc tgagcgacga ccacgcggtg caggagcgca cctggttcga
     8581 cgcgcagccc tgggacaaga cgccggccgc gctgcggcgg cggttgacgg tcggcgaggt
     8641 cgaggctgcg ggcagcgcgc tggtgcgctt cgtcggcatc gaggcctacg aggcggcggg
     8701 cggtgtggtg cggcgcgact tgttcgatga cgcgcagagc cgcttcctgt cggatccggc
     8761 actgctggag cggctggcga cggagaaact ggagacgctg gccggcaccg tgcgcgagga
     8821 aggctggaag tgggtggagg cgcggctgag cgtggactcg atggccctgc ggcagttcgc
     8881 gccctgtgcg cacgccagcc gcaagcccac ggccgccgag caggaggaac tcgatgcgct
     8941 ggagcggcgc gctgccgaac tggagcagca ggcgcaggcg ctggacgatg cgcccgagtg
     9001 gtcgcccgac gaggccgaga tgatcgacct ggaagagcag gacatcgcgg cgcggcgcaa
     9061 ggtcatccac gaagcgctga agacctggac gccggagcag aagacccatg ccggcgtcat
     9121 cgtcaccatc ggccgcgacg gcgatgtcga ggtcatgcgt gggctggtgc gcgacgagga
     9181 tcgcaaggcc atcgcggctg cggtgcgcgc gaaaggcgcg aaggagcatc gcgaccccgg
     9241 cgacgcgggc gacgacgaag gcacgccttc gatgtcgatg gcaccggagt gccgcacgcc
     9301 agggtgcagc gatgcgctga ccaagcgcct cgccgcgcac cggacgatgg cgctgcaggc
     9361 gatgctggcg cagaacacgg cggtggccct ggcctcggtg gtgcatgtgt tcgtgctgcg
     9421 tgccttcggc gccgactacc cgcgcgaagc gtcggcgttg caggtgacgc cgcagttgtc
     9481 ggcgttcgcc ctggaagcga tggccgacga cctcaagacc agtcgggctt ggcgggccgt
     9541 gcagcatgcg aaagaagtgt ggcgtgcccg cctgccagag gaccagggcg agtggcttgg
     9601 gtggctgatc aatctgcccc aggccgagtt gatcgacctg ctcgcgctgt gcggcgcgct
     9661 cacggtgaac gcgctgccgg gtgcgggcgc gacgggcagc gccaacgcca tcgcggcggc
     9721 gctcggcctg gacatggccg actggtggga gccgaccgcc gaggcgtacc tgaaccacgt
     9781 tccgaaggcg cagatcatcg cggcgctgaa ggaggccggg cccgagctgg ccggtggtgg
     9841 tgtcgaggcg atgaagaaag atgcgctggt gagcgcagcg gcatcgcggc tggccggcac
     9901 gcgctggctg cccgagccgc tgcgccgatc gccaggttga ccggaacgcg cgggcgggct
     9961 gaacccgccc gcgcgatccg ggtcggagga gtcatgtggc tgctggttgc tcgcgaaccc
    10021 cggccggacg cgcctgactg gcccggccgg cgcttgctcg cggccatcga tgcggtcgcg
    10081 tggccgctga tgtgggtgct gctgatccgg caggtgccgg ggccggctgg cctggtcggc
    10141 ccgttcgtca cagcgctcgc cgtactgctt gggctcgggc ggctgcaccg cgcgctgtgg
    10201 gtcaaccacc gctactggtt caccacctgg cgctggggca aggtgctcgg cgcgatgctg
    10261 ttgatcggca ccgtgatgaa gctgtcgatg tcggcgtagc cggagcgctt gcgagtggtt
    10321 ccgtcgcggc gggggtgtca gtgcgacacc gtgggccggc cggcgctcca ttgctcgaga
    10381 tcgatgcggc gccacgctac ggcgcgcttg gccagtcgca ccggcgacgg gaacttgtcc
    10441 tcggccatca gccggtagat cgtcgaccgg ccaagtccgg tcatgcgcac gacggcagcc
    10501 atgcggacga acacggcggg ctgaacctcg gctgcgggcg ccttcgactc ggtctgcgtc
    10561 tggctcatcg ggcggctcct gggctgcgtc tgaagagaac tctaggcgta gcgcaagagg
    10621 ccgggagcga tgactttggg ctgaaaagtg tcggagagac tcgccgatca ggttgcctct
    10681 cgtcctcaga gtcacggcgg cctcggtgtg cgtggatccc aagaagtgat caagcaagcg
    10741 cgcagtgtcg aacccagagc ctcagccgct ttgccacgga actcgacgcc gagcaagcgc
    10801 caggttggtg tttggcggtc ggcgaccatc gacggcttca gactgatctg agtccgtcgt
    10861 ggtcagggtc ctgagcgacc gcttccacca gcaccggtca ttgaaggtcg gtgacatcgg
    10921 ccggctgctt cactcctgac tccggtcgcc gaacctctgc caccgcggag gtccgacgcg
    10981 gccgaccgct tctacctact gcgactggct cagttcgacc caaagcagtc tctggatctc
    11041 ggcaagaccg gtcccagggt gacgggacgg cttccgaggc cgtccgtcac atcccggaac
    11101 tggtgagctg aatgccagtc aactgcgtcg ccaatggtca agcgccgcgg cgagttgggc
    11161 tccggttctt ctcagcgcgc gcgcaatgtt gcgtgctggt acgtcgcctt ctccatcgct
    11221 aggaagcctg ccacggacgt cggatcggcg aacgggaaga tctgggtcgc ccaatagcca
    11281 ccgacgccgt tcgcacggtc gatccagtag tagaggttgg cgaggccggc ccaggccagt
    11341 gcgccggcgg gcctgcccgt gggcgcggcc tcctcattga tcatgaacgt aagcgcccag
    11401 gtcttgggca tgccggggaa gaactccgcg tcgttggaca ggcgcggctt ggacaccggc
    11461 aggatcgagg ccgccgtcga ggtggcaccg acgccgggca gcatcttgat gtgcatcccc
    11521 ttcggcagat gattgcggga cgccatctcg acggtctcgg gtcgcaggat ctgctctccg
    11581 ctgtcgctgc gcccgtcgtt gagccacatg cggatgaact tcacgtagtc cgacgcggtt
    11641 ccgtagaggc cgtgtcctcc catgtggacc tccggatcct gtggcaacgt caccccgttg
    11701 agcgggatga gcgaaccatc gcgatcctcg cgtgcgtgaa tcgtcgcctg tcgggactgc
    11761 atggaggggg tcatcgtgaa agcggtgtca gtcatcccga gcggattgag gatcctgctg
    11821 cgcatgacat cgccgagtcg cttgccggtg atgccctcca ccaccatgcc ggcccagtcg
    11881 atgttgctgc cgtactccca gcgttcgccc ggatcaaaca gcagcggtgt cttgatcgac
    11941 ttccacgacg catcgaccac ccagggctgg ccttgctcgt tcgccaagcg cgtgtacgtt
    12001 tcgttgaaga agtcgtaggt gaagccggcg gtatgcgtga gcagatggcg cgtggtcacg
    12061 tcgcgacgcg gggggcgcag cttcggtttg ccgtcggcgc cgaagccctc tatcaccatg
    12121 atgtccccca actgggacac gtactccttg gcgggcgcgt cgaggtcgag caggccttcc
    12181 tcgaccagtt gcaggcacgc cgtgccggcg atggccttgg tggtcgagaa gatcgcgcac
    12241 acggtgtcgg gttggaacgg ttccccgctc ccggtgttgc ggacgccgcg agcgccttcg
    12301 tagatcgtgc cgcggcggtc ggtcaccgcc gccgataccc cgggcacgcg cggcgtgccc
    12361 gtggtgacgc cgtcgagcac ggtgtcgatc gctttcttca ggtctgtcat gttgtctcca
    12421 atttgattgg gtctccgccg cgggaccggc gccagagtgc aggatgggtg tgctcgggcc
    12481 gaggacccga cggaatgcag ctccagcgac gtgcctgtga cgacgggaag gtaacaacca
    12541 aaccgaacgc cggtaaccgc cgcccggcgc gccggggggc gggcgcatcc cgccagttgg
    12601 cggtcgaccc tagcggccgg tgggtggcgg gaggtcgagc gcagtcgaat gccgaacgcg
    12661 ccaagccaga aacgcgagcc ctacccgggt gcggccgatg ccgtccatgg ggtcgaacga
    12721 gagcagaccg cggatcgtct ccagcctcgc ctgcatcgtg ctgtggtgga cgccggcgat
    12781 gcgcgacgcg tcgcggatgg aggccgagcg aagcagcgca tccaaagtct gtgcggccca
    12841 ggggtgctgc atcacgctct cgagttcctc gacgtcgagc aagggtcgat cgatcgtgca
    12901 ctgggccagg agatcgacca gcccgccgta gtcgtcggcg acgacgaccg ggacctcggg
    12961 aatggcgcac aggcgcaggc tcacgagcgc ggtcgcgaac gaccggtgga ggtcgtcgac
    13021 tcccgtcgcc accccgatgc cgcaggggct ggcgtcgatc tgcttcacat cggcgcgcag
    13081 cacgcagacg tgcagcggac cgaagcgcgt gtgcaccaca tcctccagca gttcgcggtg
    13141 ctggttccac gtcgcgaaga gcggcaaggt ggcgacgcgg tacttctgcc gcggcgccaa
    13201 tccgagcttc gcggcggccg cgcggcgctg ctccacgtcg acgttggcgt cgagcagatc
    13261 gccaagccga cgcaacggat cctccgccgt gtgcccgctg cggatcgcca gcgcgagcgc
    13321 cagacgctcg aggatcatcc ggtcgttcac gcgggccggc ccgtcgcgct ccagccagac
    13381 cgtcatgccg tcggcttcga gtgcgccgac ccgcttctcg actccgccgt ccttgagaac
    13441 ctcgccgcct ggcgaaacgc gcagcgtgcg cgccgattca tggcccgcgt agcccgccac
    13501 acagcctgac agcgaggccg cagcaccgag cagtccgtga atgttgaccc cgccccgaac
    13561 caattcgtcg aagcaggcaa tgatcctcaa cgccaggctg gcgttgggat cgagggcctt
    13621 tagtcgagcc agcagttcct gcataccgcc ctcagtcccc cagcacgcgc gacatccatg
    13681 cactccgcgt ggcgatcatg ccgcgcgaca gtgcggtgtg tggcgcgaag atatcgaacg
    13741 catggaagcc acccggccag acatgcagct cggcgtcccc accggcgttc cacagggcgt
    13801 ccgcataggc gacggtctcg tcccggaata tctcggcact accgacgtcg aggaacgcgg
    13861 gcggcagatc gtgaagctgg gtcgcacggg ccggcgcggc atacaccgac acgtccggtc
    13921 caccgcgcgc gtcgccgagc agcgcgctcc acccggtctc gttgctgacg cgatcccaga
    13981 cgccgatccc gtcgaactgc cgagtggact tcgtgaggcc tcggtcgtcg agcatcgggt
    14041 agtcgagcag ctgaccgcac agacggggcc cgccgcgatc gcgcgctgcg agggccacac
    14101 cggcggcgag tccgcctccg gcgctggcgc cggcgatcag cagccgatcg agacgcacgc
    14161 cgagcgactc cgcgtgcgac gccatccact ccagcgccgc gtacacgtct tcgcgcggat
    14221 acgggtcgcg atgctcgggc gcgagtcggt actcgatcga cacgagcacg acccccagaa
    14281 gttcgacgaa atcgatcatc cggtccagcc cgctgaaccg atcgccgaac attagcccgc
    14341 cggagtgcgc gaagaggacg ccggggcgca gcccggaagc ccgctcgtga cggaagacag
    14401 atgccttgag cgcgtgtccc tcgtaccctt caaacacccg ttcctcgcag cagatgcttc
    14461 gcccaccgat cagcgtgtcc acagacggcc ccgcgtagga cacacgcatg aagtcgatca
    14521 ggtcgctggt gatggttggt ggaaacattc cccctacgat cgccaaaccc gcgcggagct
    14581 ccgggtcgaa ggcgggccgg tcccgccgtg ccggacggtc cacggcctcg atgctcccca
    14641 gggtgcgctc gttcattcca cgcatcttaa gcaaggccga atgccgcccg gcaaggctga
    14701 acgaaccggc agccactcgc ggccgaacac cgactttcgc cggtgcgcgg cgggcgggtt
    14761 ttgggagcgg tccggaagga ccgctcttgg ccgtgaccgt caggccacga tgacagctgg
    14821 tagctgccgt tggtgggcaa gccggccgaa cgaccggttc gccgggcggc ggcaggcgag
    14881 agtcgaccca ttacagccac tcagcacctt gagaagccga cattcctcgt tcgctcgtcc
    14941 aatcgctttg ggaaggtcgc gggccacata cttcgcgccg tgacacccag gcagagaggc
    15001 ctacgacctt gatgcggctc aagcactctc tcggcttggc tccgcatact cagttggcaa
    15061 gccaagccca gccgatcgcc atgttccgcc accgccgaca actacgccaa tgggcagccc
    15121 gcgtgctgct cctgtggctg ttcggcatcg cggcgggcgt tgccaatgca tgcatcacca
    15181 cggagcaggc gactccggta gccgcggcgg ccagccatct ggcggaagcg gccgaggccc
    15241 acaacgatgc ggtgcctcac gaccatgcac aggccgatcg acttgccctg ccgtcgcacc
    15301 acgcggacga ctcaacgcac atcgcaaacc tgtccaaggc gaactgccag gacttctgcg
    15361 acaaggcgac ggtgtcgatc ccgccggtga agtcggcgct ggacgacggt cagtcgcatg
    15421 cagtgatcgc catgacggcc atgaccgtcg tgccgatgcc ggccttcgcg ccggtgcagt
    15481 tgcgggtgcc gcgccgggat ggcgtgctgg ccccgccgat ccccatcgca ttcctgcgtc
    15541 tggcgctata gcgcctgaca ctggcagaca gccgtcctgg ctgct
//
LOCUS       DAOORV010000085        15245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4754_length_15245_cov_6.351284, whole genome shotgun sequence.
ACCESSION   DAOORV010000085 DAOORV010000000
VERSION     DAOORV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15245
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4754_length_15245_cov_6.351284"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..798
                     /locus_tag="PKV56_11920"
     CDS             133..798
                     /locus_tag="PKV56_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOX68565.1"
                     /translation="MKKHPVLNAAKLASVWVMAGAALLPLSALPAAAQQYKVVGADGK
                     VTYTDRPPLAAGDRISHFKAKSTPLNPDAALPVEVREAAKRYPVTLYVTPDCSPCDSA
                     RQFLRQRGIPHVEKNVTTTEDAEALVSLTGARDAPVLAIGSQVLRGMSSESWASYLDA
                     AGYPKDSKLPSTYQFPAATPLTERREVAREEASARQPALLDSAKEVRPYTPPGSGSAG
                     FRF"
     gene            complement(788..2038)
                     /locus_tag="PKV56_11925"
     CDS             complement(788..2038)
                     /locus_tag="PKV56_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX68566.1"
                     /translation="MAFSRPLPALILGQVFLHSCMTGVRVAAPLLMLRQGHPEWAVGL
                     LLGLFAAAPVATSLRAGRMADRHGYHRPMRLAVALTAVGGLLAAAAVWLTTEAPVSGW
                     PALDALRSAPNAAAFLMLSGAAMLCGVGANAGLIVIQRTAGRLAGGSGIEVTRVFSWL
                     GLAPAMSNMIGPVIAGTVIDAGGFGSAFLALSALPMISLWWMRRVPVESTADRPEPRP
                     ASSAWDLLRSPGLRLVLMVNWLLSSSWDLHAFLVPVLGHERGLSASAIGLILGTFAAA
                     VASVRLLIPWIAHHLREAPVLAGAMLTTAAVFAIYPLATSALAMAGCGALLGIALGSV
                     QPMIMTALHRLTPRQRHGEALALRSMTINGASALMPLLFGVAGSALGVAALFWMMAAA
                     MATGSLAARRVGDLPDGESSAVRT"
     gene            complement(2038..3516)
                     /gene="rmuC"
                     /locus_tag="PKV56_11930"
     CDS             complement(2038..3516)
                     /gene="rmuC"
                     /locus_tag="PKV56_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOX68567.1"
                     /translation="MENMVWITLALAVVTLLLLVWLAWRRNDDAAGQARHLALTDAVR
                     TGSERLERELRDELGRSAQGTRLELGTALGTFQQTLLNQQGDVARTQNEQIDSFRTQL
                     ATMQTLVAQSLQAASQSQTAQAQASRESQDMALKRFSDALGAQLRDMAEASERRMNDV
                     KASVELRLTALQQGNELKLEQMRATVDEKLHATLEARLGESFKQVADRLDQVHRGLGE
                     MQGLAKDVGSLNRVLTNVKSRGVFGEVQLAGLLEQVFTPDQYATNVETIPGSGARVEF
                     AIKLPGRREDGVPLWLPIDAKFPREDYERLLDAQERADRPEAEAAGKAIEMRLRAEAK
                     TIREKYVASPHTTEFAILFVPTEGLYAEALRRPGLMEALQREHRVTLAGPTTLLATLN
                     SLQMGFRTLALEKRSTEVWEVLGAVKTEFGKFGEVLAKTKKKLDEASNTIDQAQTRTN
                     VMTRTLKKVEGLSEPRTRALLPGADADADSDSTALGATPAGD"
     gene            complement(3541..4509)
                     /locus_tag="PKV56_11935"
     CDS             complement(3541..4509)
                     /locus_tag="PKV56_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOX68568.1"
                     /translation="MQAFHSDHFVLPLPEGHRFPMLKYRLLRERVAAELPDIELTEAP
                     AATDSELTLAHTPAYVEAVAAGHLTTAQQREIGFPWSPAMVERSRRSVGASIAAARAA
                     LATGFSVNLAGGTHHAHADRGSGFCVFNDVVVAARMMQAEQQRLHQRPLAVAVIDLDV
                     HQGNGTASLCRSDDTIYTLSMHGENNFPFCKESSDLDVELPDGCGDAEYLAALDVALD
                     RMWCHHARASQDGSPGLIFFLAGADPHEGDRLGRLKLTAGGLAERDRRVFAAALDRRV
                     PVSVSMAGGYGRVIEDTVDLQVNTLRLELEHWRAWQGLQPGSTGRD"
     gene            4751..5299
                     /locus_tag="PKV56_11940"
     CDS             4751..5299
                     /locus_tag="PKV56_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOX68569.1"
                     /translation="MLTAEQIVAAQKSNLDTLFGLTTKAFEGVEKLVELNLQVAKAAM
                     GEAAESTQAALSAKDAQELLALQTSLLQPAAEKAAAYSRHLYDIATATTSEMTKAAEA
                     TASDAQKKFMSVVDTAVKNAPAGSENAVALVKSAVAAANNAYESVHKASKQAVEVAEA
                     NFQAMTSTAVKAAAASKPKRTA"
     gene            5529..5888
                     /locus_tag="PKV56_11945"
     CDS             5529..5888
                     /locus_tag="PKV56_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HOX68570.1"
                     /translation="MQILLNTDPHVNGSHGMAEHLETVVKDAMGRFADHITRIEAHLA
                     DANSHAKTTPDDIHCTLEARLVGMEPVVVKDHAGTAHQAIHGAVVKLKRAVTTALEKH
                     DSRRGATPPAAIDPDAT"
     gene            complement(6102..6680)
                     /locus_tag="PKV56_11950"
     CDS             complement(6102..6680)
                     /locus_tag="PKV56_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX68571.1"
                     /translation="MSFREKMMKDPVKKWHDEHARFSRLLDYLDAQMAAFHDGGDPDY
                     DLIADIVQYLKEYSDCQHHQREDVAFAILSRRDPTLAPLVKRLAHEHRVIDNVGSALH
                     DLLLCVLNDGVISRETLEGAAATYLVYYRAHIQSEESQILPAAANLLTESDWKAVADA
                     VPSVPDPLFGAEVGLTYRAMRAQLLADAAPAH"
     gene            6949..7575
                     /locus_tag="PKV56_11955"
     CDS             6949..7575
                     /locus_tag="PKV56_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOX68572.1"
                     /translation="MRFIRIESQAPDTAAAASCNEGSGQVPKLDLPLARREFLKGSGI
                     LMGTIAATSVLATLAPSPVWALELSNLSKAEGQTLMAMGRVLYPHKKLPDAVYALLAK
                     DLDGKADAIGMLRDGIAALDQAAGGRFSKASAAKKLSVVKAIEGTPFFNTVRGQCVTS
                     LYDNDMAYAVFGYPGSAWEKGGYITRGFQDLKWLPAPPATASPPPFFG"
     gene            7648..9216
                     /locus_tag="PKV56_11960"
     CDS             7648..9216
                     /locus_tag="PKV56_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOX68573.1"
                     /translation="MAKFDNNDDSVVVIIGSGAGGGTLANELCQKGIKVVVLEAGAMQ
                     SSATFINDEWKSFSQLAWLDPRTTSGSWRIAKDFAGLPAWICKTVGGTTTHWAGASLR
                     LQEHEFRVRTTYGEIAGANLMDWPVTLKELEPYYARAEDKMGVTRTHGIPGLPGNNNF
                     KVMHAGATRLGYQEVHTGNMAINSQPRDGRTRCMQLGFCFQGCKSGAKWSTLYTELPK
                     AEKTGNMDLRPESHVSRIEHDDKGKVTGVVYFDKDGQEQRQKARIVCVAGNSIETPRL
                     LLLSASNLFKDGLANGSGQVGRNYMRHMTGSVYGMFKQPVHMYRGTTMAGIIRDEARH
                     DTRRGFAGGYEMETLALGIPFMAAFLNPGGWGSEFAEYMDNYTRLAGMWLVGEDMPRE
                     TNRVTLNTDVKDKWGNAVPNVHFDDHPNDIAMREHAFRQGQRVYEAAGAIKSFRTPPY
                     PSTHNLGTCRMGASASDSVCNAHGQTHDIANLFISDGSQFTTGGAENPTLTIVTLAIR
                     QADYIAGQMAARSI"
     gene            9272..9808
                     /gene="gloA"
                     /locus_tag="PKV56_11965"
     CDS             9272..9808
                     /gene="gloA"
                     /locus_tag="PKV56_11965"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HOX68574.1"
                     /translation="MSFTTENHPGVCAEPSVASSGFVLNHSMLRVKDPAVSLDFYTRV
                     FGMRVLRKLDFPEMKFSLYFLHRASAEQAPPEDIGERTAWTFAQHGILELTHNWGTES
                     DPAFKYHDGNAQPQGFGHICFSVPDLDAAVKWFDDNQVTYVKRPEQGKMKDVAFIKDP
                     DGYWIEIVEPGRLKNLGR"
     gene            9815..11278
                     /locus_tag="PKV56_11970"
     CDS             9815..11278
                     /locus_tag="PKV56_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HOX68575.1"
                     /translation="MAGKGSRAARAKAKTQTTPTTGGPETQATAYLRKPAHLRTRKER
                     TNAGRALRADCPHESHAGFAPAARRPDPVDLLIASSAGRIEHLVPIRYGRMLASPFAF
                     YRGAAAIMASDLSTTPGSGVIVQACGDCHLMNFGAFATPERRVIFDINDFDETHPAPW
                     EWDLKRLAASFAIASRHNGHTRTESRAAAAAVVIHYAEKLRELAAQPTLSAWYADLDY
                     ETLIELTEDEQLKRRRRKVLDKARSLDAAAEFVKLAHVDGGMPRIRDMPPLIFHPEEW
                     REPGYAEAIRENLARYRESLSAERRVLLDRYELADVAIKVVGVGSVGTLCAVGLLFAA
                     EDDPLFLQIKEARASVLEPYVKSPRYASHGERVVYGQRLMQAASDIFLGHAVGALGTH
                     FYVRQLHDVKVKPLVEIFTPQNMQGFARNTGWALARAHARSGDPALISGYIGKGTTFA
                     QAIASFAMAYEEQNQRDHAALIEAVRAGRIDASTAEA"
     gene            11630..12871
                     /gene="ppk2"
                     /locus_tag="PKV56_11975"
     CDS             11630..12871
                     /gene="ppk2"
                     /locus_tag="PKV56_11975"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOX68576.1"
                     /translation="MAKNSTDPKPPTAARARPARKTTAAVLTAEALASARSPTGKAPR
                     AQAVNRPVAAGDTTPSLNKRDIEANTVSRATTGARTEKEMALADVLARHTRDESPDQA
                     VAELKTLLASASPDDVKAIKRLLFKPETTSTLDADADIELTAGWRDGRYPYKNLLSRR
                     TYERQKYRLQVELLKLQAWVKDTGARVVILFEGRDAAGKGGTIKRMMEHLNPRGARVV
                     ALEKPSEVERGQWYFQRYIEHLPTRGEIVLFDRSWYNRAGVERVMGFCSPAEYDEFMR
                     QTPEFERHLVRSGVHLFKFWFSVSREEQRRRFKERKLHPLKQWKLSPIDLASLDKWDD
                     YTRAKEAMFLSCDTSDAPWTVIKSDCKKRARLNALRYLLHRMPYGARDLAAIGPVDPL
                     IVGRPSLVPSRGDDQLAGSGS"
     gene            13044..14018
                     /locus_tag="PKV56_11980"
     CDS             13044..14018
                     /locus_tag="PKV56_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOX68577.1"
                     /translation="MPNTAVLLWTTGAWAASFWLMAADPILFNAIGVMLCVQAMVLAA
                     YLIHEAAHNTLFGSPAANRWVGEWVGFIAGASYASFERIRQLHLRHHRDRADVTCFDF
                     KGLMERRPGVRRLLQALEWAYVPATEVLMHLQVVWRPFFVASQRRHLTRAAAMLVLRG
                     TLLIALAAWSPRALVLYALAYLLLLHVLNFFDAFHHTFEQYFVEQEQPVPMEGRDRAY
                     EQANTYSSLVSVRWPVLNLLTLNFGYHNAHHERAAVPWYRLPSAHEALFGRQAACVMP
                     LSELLATWHRNRVARVASDHYGTPAAGAGARRADGFIGAHGVSFMTVV"
     gene            14047..14949
                     /locus_tag="PKV56_11985"
     CDS             14047..14949
                     /locus_tag="PKV56_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOX68578.1"
                     /translation="MISNPVPWPDGARCAVAFTLDMDAESLLYLSYPDKAHSMVATAS
                     LLRYGPEVALPRILDTYRHFGIRQTFFVPGWCAERYPHAVEAMVRDGHEVSSHGWLHE
                     HPNELTDEEERFWLQRSLESVQKITGEKPAGWRAPLYNFSHRSTDLLIEHGIEYDASL
                     MGDDVPYLLQGDRGRLLELPSHWGLDDWPAFMHSTEFGFQMPIQPPAFAWQNWWEEFE
                     AMWEYGGLWVPVWHPFLSGRLARWRQTHRMIEKMMERGQVWFAPLRDIARHVQRCTED
                     GTWQPRVERYGHQPGLPAVPVFKG"
     gene            complement(15058..>15245)
                     /locus_tag="PKV56_11990"
     CDS             complement(15058..>15245)
                     /locus_tag="PKV56_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOX68579.1"
                     /translation="TYDYVVALRAVQTSDFMTADWAELPYALLKKVSSRIINEVRGIN
                     RVTYDVSSKPPATIEWE"
BASE COUNT         2775 a         5240 c         4894 g         2336 t
ORIGIN      
        1 gcgatggaga gcttcaaggc attccgtggt cgcgagccga gcatcgatgc cctgctgcgt
       61 caccagggca tgtcctgagc ccgagcgatc gagtccaccg cctgtgcatg aaatgcgttg
      121 agggagaaca ccatgaagaa gcaccccgtc ctgaatgccg ccaagctcgc ttccgtctgg
      181 gtgatggcag gcgcagcact gctgccgctg tctgcgctgc ccgcagcggc acagcagtac
      241 aaggtcgtcg gagccgatgg caaggtgacc tataccgatc gaccgccgct ggcagccgga
      301 gatcgaatct cgcatttcaa ggcaaagagc acgccattga acccggatgc cgcactcccg
      361 gtggaagtgc gcgaggcggc caagcgttac ccggtgacgc tgtacgtgac ccccgactgc
      421 tcgccctgcg attcggcgcg acagttcctg cggcagcgtg gcataccgca cgtcgagaaa
      481 aacgtcacca ccacggaaga tgctgaagcc ctcgtgagcc tgaccggcgc acgcgatgcg
      541 ccggtgctgg cgatcggctc gcaggtgctg cgcggcatgt cttccgaatc gtgggcgtcc
      601 tacctcgacg ctgccggata cccgaaggac tcgaagttgc cctcgaccta ccagttccct
      661 gccgcgaccc cgctgaccga acggcgcgaa gtggcgcgcg aggaagcctc ggcacgtcag
      721 cctgccctgc tcgacagcgc caaggaagtt cggccgtaca cccccccggg cagcggcagc
      781 gccggtttca ggttctgacg gccgacgatt cgccgtcggg cagatcgccg acacgacggg
      841 cagccaggct gcccgtggcc atggcggcag ccatcatcca gaacaaggca gccactccca
      901 gtgcggagcc ggcgacaccg aacagcagag gcatcagcgc gctggcgccg ttgatcgtca
      961 tcgaccgcaa ggccagcgcc tcgccgtgac gctggcgcgg cgtcagtcga tgcagggcag
     1021 tcatgatcat cggctgcacg ctccccagtg cgattcccag cagcgcgccg caccctgcca
     1081 tcgcgagtgc tgaagtcgcc agcggataga ttgcaaaaac cgcggcagtg gtcagcatcg
     1141 cgccggcaag caccggtgcc tcgcgcaagt ggtgcgcgat ccaggggatc agcaagcgca
     1201 ccgaagcgac cgcagctgca aaggtgccga ggatcaggcc gatcgccgac gcgctgagcc
     1261 cgcgctcgtg tccgagcacg ggcaccagga aggcatgcag atcccagctc gacgagagca
     1321 gccagttcac catcagcacc aggcgcaggc cggggctgcg cagaaggtcc caggcgctgc
     1381 tcgccggccg cggttcgggt cgatctgccg tcgattcgac cggtacccta cgcatccacc
     1441 acaggctgat catcgggagc gcggacaggg caagaaacgc gctgccgaat ccgcccgcgt
     1501 cgatcacggt gcctgcgatc accggaccga tcatgttcga catcgcgggc gccaggccca
     1561 gccagctgaa cacacgggtg acctcgatcc cgctgccacc agccaaccga ccggcggtgc
     1621 gctggatgac gatcaggccg gcgttggccc ctacgccgca gagcatcgcg gcgccactca
     1681 gcatcaggaa tgccgccgca ttcggcgccg accgcagcgc atcgagcgca ggccatccgc
     1741 tgaccggagc ttccgtggtg agccagacgg ctgccgcggc cagcaaaccg ccaactgcag
     1801 tcagagcgac tgcgagacgc atgggccggt ggtatccatg gcgatcggcc atgcgcccgg
     1861 cgcgcagcga ggtggccaca ggagccgctg caaacaggcc gagcagcagg cccaccgccc
     1921 actcgggatg cccctggcgc aacatcagca gcggtgctgc gacccgtaca cccgtcatgc
     1981 acgaatgcag gaacacctga cccaggatca gcgctggcag cgggcgcgaa aacgccatca
     2041 gtcgccggcc ggtgtcgcgc ccagggccgt ggagtcgcta tcggcgtccg cgtcggcacc
     2101 cggcaacaag gcacgcgtgc ggggctcgga caagccctcg accttcttga gggtgcgcgt
     2161 catcacgttc gtccgcgtct gcgcctgatc gatcgtgttg ctggcctcgt cgagcttctt
     2221 cttggtcttg gccagcacct cgccgaactt gccgaattcg gtcttcaccg cacccagaac
     2281 ttcccagacc tccgtcgagc gcttctcgag cgccagcgtg cggaaaccca tctgcaggct
     2341 gttcagcgtg gcgagcaagg tggtcggacc agccagcgtg acccggtgtt cgcgctgcag
     2401 cgcctccatc agaccggggc gcctcagcgc ctcggcgtaa agaccttcgg tgggcacgaa
     2461 caggatcgcg aattcggtcg tgtgcggcga tgcgacgtac ttctcgcgga tcgtcttggc
     2521 ttcagcacgc agacgcatct cgatcgcctt gccggccgcc tcggcctcgg gccggtcggc
     2581 gcgttcctgc gcgtcgagca ggcgctcgta gtcctcgcgc ggaaacttgg cgtcgatcgg
     2641 cagccacagc ggcacgccgt cctcacgccg gcccggcaac ttgatcgcga attcgacccg
     2701 ggcaccgctg cccggaatgg tctcgacgtt ggtcgcgtac tgatcgggcg tgaacacctg
     2761 ctcgagcaga ccagcgagtt gcacctcccc gaaaacgccg cgggacttca cgttcgtcag
     2821 cacacggttc agcgagccca cgtccttggc caggccctgc atctcgccga gcccgcgatg
     2881 gacctgatcg agccggtcgg ccacctgctt gaaactctcg ccgagacgcg cttccagcgt
     2941 ggcgtgcagc ttctcgtcga ccgtggcacg catctgttcg agcttgagtt cgttgccctg
     3001 ctgaagcgca gtcagcctca actcgacact ggccttgacg tcgttcatcc gccgctcgct
     3061 ggcctcggcc atgtcgcgca actgggcgcc cagggcatcg gaaaaacgct tcagcgccat
     3121 gtcctgcgat tcgcgcgaag cctgagcctg cgcggtctgc gactggctgg cggcctgcag
     3181 agattgcgcc accagcgtct gcatggtcgc cagctgcgtg cggaagctgt cgatctgctc
     3241 gttctgcgtg cgggcgacat ctccctgctg gttcagcagg gtctgctgga atgtgccaag
     3301 cgcggtgccc agctccagcc gcgtgccctg cgcgctgcgg cccagttcgt ctcgaagctc
     3361 gcgctccagg cgctcggagc cggtccggac ggcgtcggtc agcgcgagat gacgcgcctg
     3421 ccctgccgca tcgtcattgc gccgccaggc cagccacacg agcagcagca gggtgacgac
     3481 ggccagtgcg agcgtgatcc agaccatgtt ttccatcgat ggattgtctc aggcgggtcg
     3541 tcagtcgcgg cctgtcgacc ccggctgcag cccttgccag gcacgccagt gttcgagttc
     3601 cagacgcaac gtgttcacct gcagatccac ggtgtcctcg atcacccgcc cgtagcctcc
     3661 cgccatcgac accgaaaccg gaacccgccg atcgagcgcg gcagcaaaca cccgtcgatc
     3721 acgctcggcc aggccgccag cggtgagctt gagtcgaccc agccgatcgc cttcatgggg
     3781 gtcggctccc gccaggaaaa agatcaatcc cgggctgccg tcctgcgatg cgcgcgcgtg
     3841 gtggcaccac atccgatcga gcgccacatc gagcgcagca agatactcgg cgtcaccaca
     3901 accatcgggc agttcgacgt cgagatcgct ggattccttg cagaacggga agttgttttc
     3961 cccgtgcatc gacaacgtgt agatcgtgtc gtcgcttcgg cagagggacg cggtgccgtt
     4021 gccctggtgc acgtcgaggt ctatcaccgc cacagccaac ggacgctgat gcaaacgctg
     4081 ttgctccgcc tgcatcatcc gcgcggccac caccacgtcg ttgaatacac agaaaccgct
     4141 gccacggtcg gcatgcgcgt gatgggtgcc accggccaga ttgaccgaga agccggtagc
     4201 cagagccgcg cgtgctgccg caatgctcgc gcctacggaa cggcgtgatc gctcgaccat
     4261 cgccggggac cacggaaaac cgatctcgcg ctgctgcgcc gtggtcaggt ggccggcagc
     4321 caccgcctcg acgtaagccg gtgtatgggc cagcgtcagc tcactgtccg tggcggccgg
     4381 tgcttcggtc agctcgatat cgggcagctc ggccgcgacg cgctcgcgca acaaccggta
     4441 cttgagcatc gggaaccgat gcccttcggg cagcggcaac acgaagtgat cggagtgaaa
     4501 ggcctgcacc gcccggattg tgcggaacgc gccgccatcg cgccgagccc gcccgtttag
     4561 gaagacctcc ctagatttgc tgcattgcac caaaaacgct tgccaagcgc attcgacggc
     4621 ctatacttca aaccatgctg cactgcaaca aacgttgtgt ttcagctaga acgtgttgta
     4681 gggcaaaggc cttgagcccc acgccagtat tcatttgacc gtcatccgtc cctaccctaa
     4741 ggagatctcc atgttgaccg cagaacaaat cgtcgccgcc cagaagtcca acctcgacac
     4801 ccttttcggc ctgaccacca aggctttcga aggcgtcgag aagctcgtcg agctgaacct
     4861 gcaagtggcc aaggccgcga tgggcgaagc cgctgaatcg acccaggctg ccctgtccgc
     4921 caaggacgct caggaactgc tcgcgctgca gacctcgctg ctgcagcccg cagccgagaa
     4981 ggccgctgcg tacagccgtc acctgtacga catcgccacc gcgaccacgt ccgaaatgac
     5041 gaaggctgcc gaggcaaccg cctccgatgc acagaagaag ttcatgtctg tggtcgacac
     5101 cgccgtcaag aacgccccgg ccggcagcga aaacgctgtg gcgctggtca agtcggcagt
     5161 cgctgctgcc aacaacgcct acgaaagcgt ccacaaggcg tcgaagcaag ctgtcgaagt
     5221 ggccgaagcc aacttccagg ccatgaccag caccgccgtc aaggcagccg ctgcgagcaa
     5281 gccgaagcgc accgcctgaa tccagtccag gtactgaagg tctcgggctt cgcgcccggg
     5341 acgtcagact gaaagggccc ggtctcacga ccgggccctt tctttttctg cgcgccgatc
     5401 attgattggc gtggggttca cacgccggca ccggcagacg tccgcagacg cagcgcccgg
     5461 cagggtcaat ctgtcggcgc agtcacgctg gtcgatactg gacgctccga ccacaacgag
     5521 aagacgcgat gcaaatcctc ctgaacaccg atccgcacgt caacggcagc cacggcatgg
     5581 ccgaacacct ggaaaccgtc gtcaaggacg cgatggggcg cttcgccgat cacatcaccc
     5641 gcatcgaggc gcaccttgcc gatgcgaaca gccacgccaa gaccactcca gacgacattc
     5701 actgcacgct ggaagcccgc ctggtcggca tggaaccggt ggtcgtgaag gaccacgcgg
     5761 gcacagccca ccaggccatc cacggtgcgg tggtcaagct caagagggca gtgaccaccg
     5821 cgctggaaaa gcacgattcg cgacgaggcg ccaccccgcc ggcagcgatc gatcccgacg
     5881 cgacctgacg aggctggatc acatcaaggc gtcggcgcac cggctcgaac ggtcgcgaag
     5941 tccgccatga caggacgaac ccaactcggc gggcctgggg agccacgcgg tggcggagac
     6001 ccgggcccgc ttgccagcag gtggcgcagc gatacaggtg tgaccggcgg gtgccggcaa
     6061 cctggatcct cggcgcagtc aggtcgaccg cgcgccgcgc gtcagtgcgc tggggcagcg
     6121 tccgccagca actgcgctcg catcgcccgg taggtcaggc cgacttccgc accgaacaac
     6181 ggatcgggaa ccgacggaac cgcatcagcg accgccttcc agtcgctctc cgtcagcagg
     6241 ttcgcggccg ccggcaggat ctgagattcc tccgactgga tgtgggcacg gtaatagacc
     6301 agataggtcg cagccgcacc ctccagggtc tcgcggctga tcacgccgtc gttcagaacg
     6361 cacaacagca ggtcgtgcag cgcgcttccc acgttgtcga tgacccggtg ttcatgtgcc
     6421 agacgcttca ccagcggggc cagcgtcgga tcacgcctgg agaggatggc aaaggcgacg
     6481 tcctcgcgct gatggtgctg gcagtcggag tactccttca gatactggac gatgtccgcg
     6541 atcaggtcgt aatcgggatc gccgccgtcg tgaaacgccg ccatctgggc gtccagatag
     6601 tccagcaacc gactgaagcg cgcgtgctcg tcatgccatt tcttgaccgg gtccttcatc
     6661 atcttctccc tgaagctcat gtctgcttgg tgtgtcgcga ccgattcttc gccgccacgg
     6721 caagtcgtca ttgatctggg tcagatgtcc cgcggccaca cgggatccac cgtcagatcg
     6781 aagcccgggt agggcacgcc cgcgcgaatg ccggccgcgg atctcgcgcc gaggactttc
     6841 cctgaggggc cgccaggcac cccgtttccg gggcacaccg gtgcaacact ccggacgtcc
     6901 cgggctgcgg aaggccccga cagaaccccc gcgaacagga gatcaaccat gcgtttcatc
     6961 cgcatcgagt cgcaagcacc ggataccgcc gcggcagcct cgtgcaacga gggcagcggc
     7021 caggtgccga agctcgacct gccgctcgcg cgccgcgagt tcctgaaagg ctcgggcatc
     7081 ctgatgggca ccatcgccgc cacctcggtt ctcgcgacgc tggctccttc gccggtgtgg
     7141 gcgctggaac tgtcgaacct gagcaaggcc gagggccaga cgctgatggc gatgggccgg
     7201 gtgctctacc cccacaagaa actgcccgat gcggtgtacg cgttgctggc gaaggacctc
     7261 gatggcaagg ccgacgcgat cgggatgctg cgcgacggca tcgctgccct cgaccaggcg
     7321 gcaggcggcc gtttttccaa ggcatcggcc gcgaagaagc tgagtgtggt caaggcgatc
     7381 gaaggcacgc ccttcttcaa caccgtccgc gggcaatgtg tgacctcgct ctacgacaac
     7441 gacatggcct acgccgtctt cggctacccg ggatcggcct gggaaaaagg cggctacatc
     7501 acgcggggct tccaggacct gaagtggctg cctgcgccgc cagcaactgc gagtccgcca
     7561 ccgttcttcg gttgaaccgc cgcggcagac gaccgaagcg cggacaggaa ccgaccagac
     7621 aacaccagaa gattcccggg agacaagatg gcgaagttcg acaacaacga cgactcggtc
     7681 gtcgtcatca tcggttctgg agccggcggt ggcacgctgg ccaacgagtt gtgccagaag
     7741 ggcatcaagg tcgtcgtgct cgaggccggc gcgatgcagt ccagcgccac gttcatcaac
     7801 gacgaatgga aatcgttcag ccagctggcc tggctcgacc cgcgcacgac ctcgggcagc
     7861 tggcgcatcg ccaaggattt cgcagggctg ccggcgtgga tctgcaagac cgtgggcggc
     7921 accaccacgc actgggccgg agcgtcgctt cggctgcagg agcacgagtt cagggtgcgc
     7981 acgacctacg gcgagatcgc cggcgcgaac ctgatggact ggccggtgac gctgaaggaa
     8041 ctcgagccgt actacgcacg cgccgaggac aagatgggcg tgacccgcac ccacggcatc
     8101 ccgggtctgc cgggcaacaa caacttcaag gtgatgcatg ccggcgcgac ccggctcggt
     8161 taccaggaag tgcacaccgg caacatggcg atcaacagcc agccgcgcga cggccgcacg
     8221 cgctgcatgc aactcggctt ctgcttccag gggtgcaaga gcggcgccaa gtggagcacg
     8281 ctgtacaccg agctgcccaa ggccgagaag accggcaaca tggacctgcg tcccgagtcg
     8341 catgtctcgc gcatcgagca cgatgacaag ggcaaggtga ccggcgtcgt ctatttcgac
     8401 aaggacgggc aggagcaacg gcagaaggcg cgcatcgtct gtgtcgccgg taattcgatc
     8461 gagaccccgc gcctgctgct gctgtcggcg tcgaacctgt tcaaggatgg cctggccaac
     8521 ggatcgggcc aggtcggtcg caactacatg cgccacatga ccggctcggt gtacggcatg
     8581 ttcaagcagc cggtgcacat gtaccgcggc acgacgatgg ccggcatcat tcgcgacgag
     8641 gcgcgccacg acacccgacg cggcttcgcc ggcggctacg agatggagac gctggcgctg
     8701 ggcatccctt tcatggcggc cttcctgaat ccggggggct ggggcagcga gttcgccgag
     8761 tacatggaca actacacgcg gctggccggc atgtggctgg tcggcgagga catgccgcgc
     8821 gagaccaacc gggtcacgct gaacacggac gtgaaggaca agtggggcaa cgccgtgccc
     8881 aacgttcact tcgacgacca cccgaacgac atcgcgatgc gcgaacacgc gttccgccag
     8941 ggccagcgcg tctacgaggc agccggagcg atcaagtcgt ttcgcacacc gccctaccct
     9001 tcgacgcaca acctgggcac ctgccgcatg ggcgcctcgg ccagcgacag cgtgtgcaac
     9061 gcgcatgggc agacccacga catcgccaac ctgttcatca gcgacggcag ccagttcacc
     9121 accggcggag cggaaaatcc gacgctgacg atcgtgacgc tcgccatccg ccaggccgac
     9181 tacatcgccg ggcagatggc ggcacgcagc atctgacctc cagcgcggca cagagccgcc
     9241 ggcaacatcc atcgaccttc aggatcaaac catgagcttc accacagaga accaccccgg
     9301 cgtctgcgcc gagcccagcg tggcttcaag cgggttcgtc ctcaaccaca gcatgctgcg
     9361 cgtcaaggac ccggcggtct cgctggattt ctacacccgc gtcttcggca tgcgcgtgct
     9421 gcgcaagctc gacttccccg agatgaagtt ctctctgtac ttcctgcacc gcgcttcggc
     9481 cgaacaggcg ccgcccgagg acatcggcga gcgcaccgcg tggacctttg cccagcacgg
     9541 catcctggag ctgacgcaca actggggcac cgaaagcgat ccggccttca agtatcacga
     9601 tggcaacgcg caaccacagg gcttcggcca catctgtttc tcggtgcccg acctcgatgc
     9661 ggccgtgaag tggttcgacg acaaccaggt cacctacgtc aagcgccccg agcagggcaa
     9721 gatgaaggac gttgccttca tcaaggaccc ggacggctac tggatcgaga tcgtcgaacc
     9781 cggtcgcctc aagaacctgg gccgctagct cgtcatggcg ggcaaaggct cgcgcgcggc
     9841 aagggcgaag gcgaagacgc agacgacccc gaccaccggg gggcccgaaa cgcaggccac
     9901 ggcttatctg cgcaagccgg ctcacctgcg cacgcgcaag gagcgaacga atgccggccg
     9961 tgcgctgcga gcagactgcc cgcacgagtc tcacgccggc ttcgcgccgg cggcccgccg
    10021 ccccgacccg gtcgacctgc tgatcgcatc gagcgccggc cgcatcgagc acctggtgcc
    10081 gatccgctac ggtcgaatgc tcgcttcgcc attcgccttc taccggggag ctgccgccat
    10141 catggcgagc gacctgtcga ccacaccggg cagcggcgtg atcgttcagg cctgcggcga
    10201 ctgccacctg atgaatttcg gtgcgttcgc cacgcccgag cggcgcgtca tcttcgacat
    10261 caacgacttc gacgagaccc accccgcgcc ctgggaatgg gacctgaagc gcctggcagc
    10321 gagcttcgcg atcgcgagtc gacacaacgg gcacacacgc accgaaagtc gagctgcagc
    10381 cgcggccgtg gtcatccatt acgcagagaa gctgcgtgag ctcgccgcac aaccgaccct
    10441 gagcgcctgg tacgccgacc tcgattacga gaccctgatc gagctgaccg aggacgagca
    10501 gctcaagcgt cgccgcagga aggtgctgga caaggccaga tcgctcgacg cggcggcaga
    10561 gttcgtcaag ctggcccacg tggacggcgg aatgccgcgc atccgcgaca tgcctccgtt
    10621 gatctttcac cctgaagagt ggcgcgagcc cggctatgcc gaggcgatcc gcgagaacct
    10681 ggctcgctat cgcgagtcgc tgtcggccga gcgccgcgtg ctgctcgacc gctacgaact
    10741 cgcggacgtg gccatcaagg tggtcggcgt cgggagcgtc gggacgctct gcgcggtcgg
    10801 actgctcttt gccgccgaag acgatccgct gtttctccag atcaaggaag cccgcgcctc
    10861 ggtgctcgag ccctacgtca agtcaccgcg ctacgcatcg catggcgagc gggtggtcta
    10921 cgggcagcgc ctgatgcagg ccgcatcgga catcttcctg ggtcatgcgg tcggcgccct
    10981 gggcacccac ttctacgtgc ggcaactgca cgacgtgaag gtgaagccac tcgtcgagat
    11041 cttcacgccg cagaacatgc agggcttcgc gcgcaacacc ggatgggcac tggcacgtgc
    11101 ccatgcgcgc tcgggcgacc cggcgctgat ctcgggctac atcggcaagg gaaccacgtt
    11161 cgcgcaagcc atcgcctcgt tcgccatggc ctacgaggag cagaaccaga gggatcacgc
    11221 cgcgctcatc gaagccgtgc gagccggtcg gatcgacgcg agcacggcgg aagcgtgacc
    11281 cagcggtccg ggactggttg aagcgcatgg aagcgggcgc ctgagagtcc cccccgggaa
    11341 gcaatggccg cctgcgcaac atttcgctgc gggttccttg cgcaaggaat tcaaacgccc
    11401 gttctcccga acgtcgatgc acattgagga cctgttcttc atccgggtag ccctcgtcgg
    11461 ccgcatcgag cggcaaagcc cgcgcagagc gcacgctccg gccggccgac ggcggccagg
    11521 cgtgtcccgc gaccatcgcg gcaccacgct ggtttgacat cgctcaacac ctgcaccgat
    11581 gcaatcgcgg gatcatcaaa ggttcacaga tctgtcactg gagcacggca tggccaagaa
    11641 ttcgaccgac cccaaaccgc cgaccgcagc ccgtgcgcgg cccgcgcgca agacgaccgc
    11701 cgcagtgctg acagcggagg cgctggcatc ggccaggtcg cccaccggca aggcgccccg
    11761 cgcccaggcc gtgaaccgcc cggtggcggc gggcgacacc acgccgtcgc tgaacaagcg
    11821 cgacatcgag gccaacaccg tctcgcgcgc caccaccggc gcccgcaccg agaaggagat
    11881 ggcactggca gacgtgctgg cccgccacac gcgtgacgaa tcgcccgacc aggcggtggc
    11941 cgagctgaag acgctgttgg ccagcgcgtc ccccgacgat gtcaaggcga tcaagcgcct
    12001 gctgttcaag cccgagacca catcgacgct cgacgccgac gccgacatcg agctgactgc
    12061 cggctggcgc gatggccgct acccgtacaa gaacctgctg tcgcgccgga cctacgagcg
    12121 ccagaagtac cgcctgcagg tcgagctgct caagctgcag gcctgggtca aggacactgg
    12181 tgcgcgcgtg gtgatcctgt tcgaggggcg ggacgcggcc ggcaagggcg gcaccatcaa
    12241 acgcatgatg gagcacctga acccgcgcgg cgcgcgcgtc gtggcgctgg aaaaacccag
    12301 cgaggtcgag cgcggccagt ggtacttcca gcgctacatc gagcacctgc ccacgcgcgg
    12361 cgagatcgtg ctgttcgacc gcagttggta caaccgcgcc ggcgtcgagc gggtgatggg
    12421 cttttgcagc cccgccgagt acgacgagtt catgcgacag acaccggagt tcgagcgcca
    12481 tctggtgcgc agcggcgtgc acttgttcaa gttctggttc tcggtcagcc gcgaggaaca
    12541 gcgccgccgc ttcaaggagc gcaagctgca tccgctcaag cagtggaagc tgagcccgat
    12601 cgacctggcc agcctcgaca agtgggatga ctacacccgc gccaaggagg caatgttcct
    12661 gagctgcgac acgtccgacg cgccttggac ggtgatcaag agcgattgca agaagcgcgc
    12721 gcggctgaac gcgctgcgct acctgctgca ccgcatgccc tatggcgcca gggacctggc
    12781 cgcgatcggc ccggtcgacc cgctgatcgt cggccggccg tcgctggtgc ccagccgcgg
    12841 cgacgaccag ctggccggca gcggcagctg aacccagggg cgctttcgcg cctcagagtc
    12901 gacctcggca gcgtcgcgca ggcagcggag tgcggccaat ggcacagcac ctgctagttg
    12961 tgctgaaggc acaagcagcg cgcaccacga tgacatcgac gaccaccccg acccctcccc
    13021 tttttcgcga agcggagggc gcggtgccca acacggcggt gctgctctgg acgaccggcg
    13081 catgggcggc gtcgttctgg ctgatggcgg ccgatcccat cctgttcaac gcgatcggcg
    13141 tcatgctgtg cgtgcaggcc atggtgctgg cggcctacct gattcacgag gcggcccaca
    13201 acaccctgtt cggctcgccc gcggccaacc gctgggtggg agagtgggtg ggcttcatcg
    13261 ccggggcgag ctatgcgtcg ttcgagcgca ttcgccagct gcacctgcgc caccaccgcg
    13321 accgcgccga cgtgacctgc ttcgacttca aggggctgat ggagcgccgg ccgggggtgc
    13381 ggcgactgct gcaggcactg gagtgggcct acgtgccggc caccgaggtg ctgatgcacc
    13441 tgcaggtggt gtggcgcccg ttcttcgtgg cgtcgcaacg ccgccatctg acacgggcgg
    13501 ctgccatgct ggtgctgcgc ggcacgctgc tgatcgcgct ggctgcgtgg tcgccccggg
    13561 cgctggtgct gtacgcgctg gcctacctgc tgctgctgca cgtgctcaac ttcttcgacg
    13621 cgttccacca caccttcgag cagtacttcg tcgagcagga gcagcctgtg ccgatggaag
    13681 gacgcgaccg cgcctacgaa caggccaaca cctattcgag cctggtctcg gtgcgctggc
    13741 cggtgctgaa cctgctgacc ctcaacttcg gatatcacaa cgcccaccac gagcgggccg
    13801 cggtgccgtg gtaccggctg ccgtccgcgc acgaggcgct gttcggccgg caggcggcct
    13861 gcgtgatgcc gctgtcggag ctgctggcca cctggcaccg caaccgggtc gcgcgcgtcg
    13921 cctccgacca ctacggcacg cccgcagccg gggccggggc gcgtcgcgcc gacggcttca
    13981 tcggcgccca cggtgtgtcg ttcatgacgg tcgtgtgagc cggcacatca aactcagagg
    14041 tcactgatga tcagcaaccc ggttccctgg cccgatggcg cgcgctgcgc ggtggccttc
    14101 acgctagaca tggatgccga gagcctgctg tacctgtcgt accccgacaa ggcccacagc
    14161 atggtcgcca ccgcctcgct gctgcgatac ggccccgagg tggcgctgcc tcgcatcctc
    14221 gacacctacc gccacttcgg catccggcag accttcttcg tgccgggctg gtgcgctgaa
    14281 cggtatccgc atgcggtcga ggccatggtg cgcgacgggc acgaggtgtc gtcgcacggc
    14341 tggctgcacg agcacccgaa cgagctgacc gacgaggaag agcgcttctg gctgcagcgc
    14401 agcctcgagt cggtgcagaa gatcaccggc gagaagcccg ccggctggcg cgcgccgctc
    14461 tacaacttct cgcaccgctc gaccgatctg ctgatcgagc acggcatcga gtacgacgct
    14521 tcgctgatgg gcgacgacgt gccctacctg ctgcagggcg atcgcggccg gctgctcgag
    14581 ttgccgtcgc actggggcct cgacgactgg cccgccttca tgcacagcac cgagttcggc
    14641 ttccagatgc ccatccagcc gccggccttc gcgtggcaga actggtggga ggagttcgag
    14701 gcgatgtggg agtacggcgg cctctgggtg ccggtgtggc acccgttcct gtcggggcgc
    14761 ctggcccgct ggcggcagac ccaccgcatg atcgagaaga tgatggaacg cgggcaggtg
    14821 tggttcgcac cgctgcgcga catcgcccgc cacgtgcagc gctgcaccga agacggcacc
    14881 tggcagccgc gcgtcgaacg ctacgggcac cagccggggc tgccggcggt gccggtgttc
    14941 aagggctgaa cttcaggtca cttctcgacc aggcacctgg gggcgccggc attgaccgtc
    15001 tgcgcgtgga atcaacgaat caggtcggtt cttcaacgga cctcgatcaa gcggaaccta
    15061 ctcccactcg atcgtcgccg gcggcttgct gctgacgtcg taggtcacgc ggttgatgcc
    15121 gcgcacctcg ttgatgatgc ggctgctgac cttcttcagc agcgcgtacg gcagctcggc
    15181 ccagtcggcg gtcatgaagt cgctggtctg cacggcgcgc agcgccacca cgtagtcgta
    15241 ggtgc
//
LOCUS       DAOORV010000086        15149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4803_length_15149_cov_8.098847, whole genome shotgun sequence.
ACCESSION   DAOORV010000086 DAOORV010000000
VERSION     DAOORV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_15149_cov_8.098847"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1020
                     /gene="puhE"
                     /locus_tag="PKV56_11995"
     CDS             166..1020
                     /gene="puhE"
                     /locus_tag="PKV56_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
                     /protein_id="HOX68580.1"
                     /translation="MSQYVLPVTYTLFVWWFSTGVILYLNGLPRWTFKWTMVGTTFVL
                     VMALIGLVATRDDTRTTGAYLAFTFGLLVWAWQEVAFLLGYVTGPRRSDCPTGSTGWR
                     RARFAFMAVMHHELALVVLGAAVLAATWGGANQTGMWTFLILWVMRQSAKLNVFLGVR
                     NLNEGFLPDHLKYLQTYFTRRPMNRLFPVSVISSTIVAALIWQEASVLGLDAFDATSL
                     TFAATLLTLAVLEHWFLVLPLPSEKLWDWGLRSRSPEVADQPPAAPPEDDGPSGDLKL
                     RAAQRVFR"
     gene            complement(1025..1903)
                     /locus_tag="PKV56_12000"
     CDS             complement(1025..1903)
                     /locus_tag="PKV56_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX68581.1"
                     /translation="MAAPQHLRALAGMVVVTLLWSTAGVVTRQLESARSFEATFWRSA
                     VTCLTLAVYLLMAHRQGAIPSIRWRDPALWLSGLMWAVMFTCFMVALTLTSVANVLIT
                     MSVAPLVTAVLARFMLGQPVATRTWGVIVSAGAGIAWMYVHSLSADPKAVIGTAVALG
                     VPIAAAINWNTLQRSGGAVDLVPALLVGALLSSFVALPFALPLQASPADVAWLAGLGV
                     FQLAVPCVIAVRLARLLPAPEIALLALLEIVFGIGWAWWGAGEAPAADVLIGGAIVLA
                     SLTFDQLWIYRSGASR"
     gene            complement(1917..3746)
                     /gene="typA"
                     /locus_tag="PKV56_12005"
     CDS             complement(1917..3746)
                     /gene="typA"
                     /locus_tag="PKV56_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOX68582.1"
                     /translation="MTQQIRNIAIIAHVDHGKTTMVDQLLRQSGTFAEHEKVVDTVMD
                     SNAIERERGITILAKNCAVSWEGTHINIVDTPGHADFGGEVERALSMVDGVVLLIDAQ
                     EGPMPQTRFVTKKALALGLKPIVVVNKVDKPGSKPDAVINAAFDLFDKLGANDEQLDF
                     PVVYASGINGWTSLTEGAPGEQWGPDMSALFNTILKHVPPNTGDPAAPLQLQISALDF
                     STFVGRIGVGRISQGTIKPSMDVLVMEGPDGKAVKGRVNNVLTFQGLDRVQVTEAGPG
                     DIVLINGLEDINIGVTITDRENPMPLPMLKVDEPTLTMNFCVNTSPLAGREGKYVTSR
                     QIWDRLQKELQSNVALRVSETGEDGIFEVMGRGELHLTILLENMRREGYELAVSKPRV
                     MFKEINGEKCEPMELVTADVEEIHQGGVMQALGLRKGEMVNMEPDGRGRVRLEYRIPA
                     RGLIGFSNEFMNLTRGSGLIASIFDGYEPHKGEIGGRKNGVLISMDDGEIFTYALGKL
                     DDRGRMFVKPNDPVYEGMIVGIHSRENDLVVNATRTKQLTNFRVSGKEDAIKITPPID
                     LTLEYGVDFIDDDELVEITPKSVRVRKRFLKEHERKKASREAA"
     gene            complement(3743..4747)
                     /gene="truB"
                     /locus_tag="PKV56_12010"
     CDS             complement(3743..4747)
                     /gene="truB"
                     /locus_tag="PKV56_12010"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOX68583.1"
                     /translation="MKFAARPRIPRRAVHGVLLLDKPMGLSSNDALMKAKRLYRAEKA
                     GHTGTLDPLATGLLPLCFGAATKFSQVGLDADKIYRATLRLGITTTTADAEGEVLERT
                     EVHVTRELFEAACNRFVGEISQVPPMHSALKRDGKPLYEYARAGIEVEREPRQVTIHR
                     IDIPQEQHGGRDNGKGDEWAIEVHCSKGTYIRTLAEDIGRALGCGAHLAALCRTGSGP
                     LTLAGAQTLESLAAMTEAERDALLKDTDSLIADWPVVRLDDEGAGRFLSGVRRRLALP
                     NTPRVRVYGPQAKAFLGSGHITAGELISTRLLSPLEVQGLLAPAEPAPIRDMNEVSTT
                     "
     gene            complement(4752..5117)
                     /gene="rbfA"
                     /locus_tag="PKV56_12015"
     CDS             complement(4752..5117)
                     /gene="rbfA"
                     /locus_tag="PKV56_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOX68584.1"
                     /translation="MKHKRSIPNRGFRVADQIQRDVAVLIRDLKDPRIGMVTLHAVEV
                     TPDYAHAKIFFSVLVGDPQECEVALNEAAGFIRNGLFKRLQIHTVPTLHFHFDRTTER
                     AAELSLLIAQANATRAKED"
     gene            complement(5209..8049)
                     /gene="infB"
                     /locus_tag="PKV56_12020"
     CDS             complement(5209..8049)
                     /gene="infB"
                     /locus_tag="PKV56_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOX68585.1"
                     /translation="MAVTTVAQLAAELNRPAAALLDQLQSAGVSKASTDDALTDSDKE
                     RLLDYLRATHGTAGAERKKITLTRKSTSEIKQADSSGKARTIQVEVRKKRTFVKRDDA
                     PAGGESATEAAEVNNEELMRLEHEAQAQAELLRKQEAELAERQRQRDEQARLEREAAE
                     ARAQAEKAAAAAAAAAVEAAARSAGSEAAVPVAADAAAEVPPAVVEPVKPVLRVIKAA
                     DVVDEDKQRAIDLAKRRKAAEDEAAAIRAMMSAPKKVLNAKKPEEAPKPVEAGKEGIK
                     GTIHKPAVAPGAPGAVVAKPGDKKSVKSEKLSSSWADDAAKKRGLKTRNDAPGGARAG
                     WRAPRGGRRGERSDGSVSTFVAPVEQQVQEVHVPETISVADLAHKMSVKASEVIKQLM
                     KLGQMVTINQQLDQETAMILVEEMGHKALAAKLDDHDAFLEEDVEGESEHEMLPRAPV
                     VTVMGHVDHGKTSLLDYIRTSRVAAGEAGGITQHIGAYHVETPRGMITFLDTPGHAAF
                     TAMRARGAKATDLVILVVAADDGVMPQTKEAIAHAKAAGVPLVVAMNKIDKPGANLER
                     LKSELVAEEVVPEEFGGSSPFVAVSAKTGQGIDDLLEQVLLQAEVLELTAPKDSMAKG
                     LVIEAKLDKGRGPVATVLVQSGTLKRGDVVLAGSTYGRVRAMLDENGKPIAEAGPSIP
                     VEIQGLTEVPQAGDEFMVLADERRAREIATFRSGKYRDVKLAKQQAAKLENMFDNVGE
                     GAAQSLPLIIKADVQGSQEALAQSLLKLSTAEVKVQVVHGAVGGISESDVNLAIASKA
                     VIIGFNVRADAGARKLAEHNGVDIRYYNIIYDAVDEIKAAMTGMLAPEQREEVIGTAE
                     IRTVFVASKIGTVAGSMVTAGLVRRGARFRLLRDNVVVYTGEIESVRRLKDDVKEVAS
                     GFECGIKLKNYNDIAEGDQLEFFEVKEVARTL"
     gene            complement(8120..9598)
                     /gene="nusA"
                     /locus_tag="PKV56_12025"
     CDS             complement(8120..9598)
                     /gene="nusA"
                     /locus_tag="PKV56_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOX68586.1"
                     /translation="MNRELLMLVDAISREKSVDREVVFGAVEAALASASKKLHGGEVD
                     MRVTVDRDTGAYETFRRWHVVADDAGLQLPDSEILHFEALEQIPDIEVDDYIEEPVES
                     VSIGRIGAQAAKQVILQKIRDAEREQLLNDFLSRGEKIFVGTVKRLDKGDVIVESGRV
                     EGRLRRSEMIPKENLRIGDRVRAYILEVDSTLRGPQIILSRSAPGFMIELFAQEVPEI
                     EQGLLEIKSCARDPGSRAKIAVQSHDKRVDPIGTCVGVRGSRVNGVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVQSIVVDEERHAMDVVVDEENLAIAIGRGGQNVRLASEMT
                     GWRINIMTAEESSEKQAVESDSLRKLFVEKLDVDAEVADILIAEGFSSLEEVAYVPMQ
                     EMLEIEGFDEDTVNELRTRAKDALLTMEIAREESVSDVSQDLRDLEGLDPELIPKLAD
                     GGVHTRDDLADLAVDELIEISGMDDEKAKALIMKAREHWFTT"
     gene            complement(9595..10158)
                     /gene="rimP"
                     /locus_tag="PKV56_12030"
     CDS             complement(9595..10158)
                     /gene="rimP"
                     /locus_tag="PKV56_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOX68587.1"
                     /translation="MNWQLAIEKTVTGLGYDLVDTERSPKGLLRVYIDKPAPVSPGST
                     PAGSEFITVEDCERVTRQLQHVLEVEACAYERLEVSSPGLDRPLKKPADFLRFSGQRV
                     DLALKLAFKGRKHFQGVLEPLGDGWRLVFQGDGVDQALDFAFDEVREARLVPVVDFKG
                     RRFAAPVEVPTEQVESAANEMDGDRRR"
     gene            complement(10409..11611)
                     /locus_tag="PKV56_12035"
     CDS             complement(10409..11611)
                     /locus_tag="PKV56_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOX68588.1"
                     /translation="MDQVKQGTDAFFILLGAIMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKILVDFSVSTVMYFFIGYTVAYGIGFLGSAETLMEKNGFELVRFFFLLTFAAAIPAI
                     VSGGIAERARFGPQLIATALLVGAAYPLLEGVVWGGKFGLQGQIKALTGAEFHDFAGS
                     VVVHAFGGWVGLVAVLMLGARSNRYRKDGGISAHPPSSIPFLALGAWVLAVGWFGFNV
                     MSAQTIEKISGLVAVNSLMAMAGGTLVAVLMGRNDPGFVYNGPLAGLVAVCAGSDVMH
                     PAGALVVGCVASAIFVSMFTLTQNRWKIDDVLGVWPLHGLCGAWGGIACGIFGSQSLG
                     GVGGVSLGAQVVGTLLGVSWALIAGVIVYGGLKAVTSLRLTLEEEYEGADLSIHRIGA
                     TPEREANW"
     gene            complement(11800..13257)
                     /gene="sbcB"
                     /locus_tag="PKV56_12040"
     CDS             complement(11800..13257)
                     /gene="sbcB"
                     /locus_tag="PKV56_12040"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOX68589.1"
                     /translation="MDPFSFFWHDYETFGVVPRRDRPAQFAGVRTDADLQEIDEPVTL
                     YCQPPLDCLPDPESCLLTGITPQICAERGVPEHAFAAAIEAQLARPGTVGVGYNSIRF
                     DDEVTRFLFWRNLIDPYAREWQNQCGRWDLLDVVRCAYALRPDGIAWPRHDDGRPSFK
                     LEHLTAANGLAHEAAHDALSDVRATIALARLLKDRQPKLWDFCLRLRKKDAVIEEMAS
                     AQNHGRPFLHVSGMYPPDRGCIALVWPLAPHPTNKNEVIVWDLAEDPGVLRALDADAI
                     RQRMFSRADALPEGVTRLPIKTIHLNKSPVVIANLKTLSAEMATRWGVDVVQACRHAE
                     VAAHQAPLLAGLWPDVFERPATTGTPDVDEDLYGGFVGNDDRRLLQRLRALDPQALAG
                     QRPVFADDRLGELLFRYRARNFPDTLSGAEAQQWLELRLRRLHGGEGGGLTLEAFFER
                     IDQLSSQFEDSGDERAQDILGALVDHATEIAPELP"
     gene            13313..>15149
                     /gene="acnA"
                     /locus_tag="PKV56_12045"
     CDS             13313..>15149
                     /gene="acnA"
                     /locus_tag="PKV56_12045"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOX68590.1"
                     /translation="MNRSTARSKSPFATKHAFAKTLKTFVTDSGIEGGYYSLPALAKI
                     IPNVARLPVSLRIVLESVLRNCDGRKVTPEHVERLAAWKPNAGRTDEIPFVVARVVLQ
                     DFTGVPLLADLAAMRNVAAAMGKDPKTIEPLVPVDLVVDHSVMIDHYGNRKALDLNMR
                     LEFQRNRERYEFMKWGMQAFNTFGVVPPGFGIVHQVNLEYLARGVHKTPKGQPNAGVY
                     YPDSLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGFELTGRLREG
                     VTATDLVLTVTEILRQAKVVGKFVEFFGEGTASLDLPDRATIANMAPEYGATMGFFPV
                     DEKTIDYFRGTGRTKREIEAFEAYFRAQKLFGVPKRGDIDYSAVVTLDLGRVAPSLAG
                     PKRPQDRIELGNVKAQFSSLYSQPPTQNGFNQPAGSLYFRHLARSSNAARGTPATAPA
                     LIPGAARDVVEMVGNRSTLEASLSESEPAHPVDGDISIGNGDVLIAAITSCTNTSNPS
                     VLLAAGLLAKKAVEAGLKVQPHIKTSLAPGSRIVTEYLEKAGLLPYLEKLGFYLAGYG
                     CTTCIGNAGDLRPEFNDVITENNLVCAAVLSGNRNFEARIHPNLKA"
BASE COUNT         2593 a         5049 c         4739 g         2768 t
ORIGIN      
        1 tgctcggcgg catcaagcgt gccgcgctga cggtgtctgc ggtggcgatg ttcgctcgcc
       61 tgtactgcct gccggcaaca ccgaacagcc tgccggtcga cgttcgcgtg gccccggcct
      121 ggtgattcac cgacaagccg cctagcttgt ccggggtcga atccgatgtc gcagtacgtg
      181 ctcccggtca cctacaccct gtttgtctgg tggttcagca cgggcgtgat cctctacctc
      241 aacggcctgc cgcgctggac cttcaagtgg acgatggtgg gcacgacttt cgtgctggtg
      301 atggcgttga tcggactcgt ggccacgcgc gacgacaccc gaacgaccgg cgcttacctc
      361 gccttcacgt tcgggttgct ggtctgggca tggcaggagg ttgcattcct gctgggctat
      421 gtgacgggtc cgcgtcgcag cgattgtcca accggctcga caggttggcg tcgggcacgt
      481 ttcgcgttca tggcggtgat gcaccatgaa ctggcactgg tcgttctggg tgcagcggtg
      541 ctggcggcga cctggggcgg agccaaccag accggcatgt ggacctttct gatcctgtgg
      601 gtcatgcgcc agagcgccaa gctgaatgtc ttcctgggcg tgcgcaacct caatgaaggc
      661 ttccttccgg atcacctgaa gtacctgcag acctacttca cccgccgtcc gatgaaccgg
      721 ttgtttcctg tctccgtcat ctcgtcgacc atcgttgccg cgctgatctg gcaggaagca
      781 tccgtgctcg gcctcgatgc gttcgatgcg acctcattga cgttcgcagc cacattgctg
      841 acgctggctg tgctcgagca ctggttcctg gtactgcctc tgccgtccga aaaactgtgg
      901 gactggggac ttcgatccag gagtcccgag gtggctgatc agccgccagc ggcccccccg
      961 gaagatgacg ggccatccgg tgatttgaag ctgagggcag cccagcgcgt ctttcgataa
     1021 gtgctcagcg cgacgcgccg gacctgtaaa tccacagttg atcgaaggtc aggctcgcaa
     1081 gcacgatggc cccgccgatc agcacgtccg cagcgggcgc ttctcctgcc ccccaccagg
     1141 cccagccgat cccgaagacg atttcgagca gtgccagcaa ggcgatttcc ggagctggta
     1201 gcaggcgagc cagacgaacc gcgatcacac acggcacggc caactgaaac accccgagtc
     1261 cagccagcca ggccacgtcg gctggggaag cttgcaatgg caacgcaaag ggcagcgcga
     1321 caaagctgga caagagcgca ccaacaagca gtgctggcac cagatcgacc gcaccaccac
     1381 tgcgctgcaa ggtgttccag ttgatggcgg ccgcgatggg cacgcccagc gccacggcgg
     1441 tgccgatgac ggccttcgga tcggcactca gactgtgaac gtacatccac gcgatgccgg
     1501 cacctgccga aacgatgacc ccccaggtac gcgtcgcgac aggctggccc agcatgaagc
     1561 gcgcgagcac tgcagtcacg agtggagcca cgctcatcgt gatcagcaca ttggccaccg
     1621 acgtcagcgt cagggcgacc atgaagcacg tgaacatcac ggcccacatc aacccggaca
     1681 gccacagcgc agggtcacgc caccgtatcg atgggatggc gccttgtcgg tgtgccatca
     1741 ggaggtacac ggcaagcgtc aggcacgtga cggcgctgcg ccagaaggtc gcctcgaaac
     1801 tgcgggcgga ttcgagctgg cgggtcacca cacccgccgt gctccacagc agcgtgacga
     1861 ccaccatccc ggccagcgcc cgcagatgct gcggcgcagc catcacgcca atggggtcag
     1921 gcggcttcgc gcgacgcctt cttgcgttcg tgctctttca ggaagcgctt gcgcacacgc
     1981 accgacttcg gcgtgatctc gaccagctcg tcgtcgtcga tgaagtccac accgtactcc
     2041 agcgtcaggt cgatcggcgg cgtgatcttg atcgcgtctt ccttgccaga aacgcggaag
     2101 ttggtcagct gcttggtgcg cgttgcgttg acgacgaggt cgttctcgcg gctgtggatg
     2161 ccgacgatca tgccttcgta gaccgggtcg ttcggcttga cgaacatgcg gccgcggtcg
     2221 tccagcttgc ccagggcgta ggtgaagatc tcaccgtcgt ccatcgagat cagcacgccg
     2281 ttcttgcggc caccgatctc gcccttgtgg ggctcgtagc cgtcgaagat cgaggcgatc
     2341 aagccggagc cacgggtcag gttcatgaac tcgttgctga agccgatcag gcctcgcgcc
     2401 ggaatgcggt actcaaggcg cacgcggcca cggccgtcgg gctccatgtt gaccatctcg
     2461 cccttgcgca gacccagtgc ctgcatcacg ccaccttggt ggatttcttc gacgtcggcg
     2521 gtgaccagct ccatcggctc gcacttctcg ccgttgatct ccttgaacat gacgcggggc
     2581 ttggaaacag ccagctcgta gccctcgcgg cgcatgttct ccagcaagat ggtcaggtgc
     2641 agttcgcccc ggcccatcac ctcgaagatg ccgtcttcgc cggtttcgct gacgcgcagc
     2701 gcgacgttgc tttgcagttc tttttgcagc cggtcccaga tctggcggct ggtgacgtac
     2761 ttgccttcac gacccgccag cgggctcgtg ttgacacaga agttcatcgt cagcgtcggc
     2821 tcgtcgacct tcagcatggg cagcggcatc gggttctcac ggtcggtgat cgtcacgcca
     2881 atgttgatgt cttcaaggcc gttgatcagc acgatgtcgc ccgggccggc ttcggtgacc
     2941 tgcacgcggt ccaggccctg gaaagtcagc acgttgttca cacgaccttt gacggccttg
     3001 ccgtccgggc cctccatgac cagcacgtcc atcgacggct tgatggtgcc ctggctgatg
     3061 cggcccacgc cgatgcggcc gacgaaggtc gagaagtcga gcgccgagat ttgcagttgc
     3121 agcggggcgg ccgggtcacc ggtgttcggc ggcacgtgct tcaggatggt gttgaacagg
     3181 gccgacatgt cggggcccca ttgttcgccg ggcgcgcctt cggtcaacga cgtccaaccg
     3241 ttgatgcccg aggcgtagac cacggggaaa tcgagctgtt cgtcgttggc accgagcttg
     3301 tcgaacaggt cgaaagcggc gttgatgaca gcgtcgggct tcgaacccgg cttgtcgacc
     3361 ttgttgacca cgacgatggg cttgaggccc agagccagcg ccttcttggt cacgaagcgc
     3421 gtttgcggca tcgggccttc ctgcgcatcg atcagcagca ccacgccatc gaccatcgaa
     3481 agcgcgcgct cgacttcacc accgaagtct gcgtggcccg gggtgtcgac aatgttgatg
     3541 tgggtgcctt cccagctgac cgcgcagttc ttggccagga tggtgatgcc gcgctcgcgc
     3601 tcgatcgcgt tgctgtccat cacggtgtcg accaccttct cgtgctcggc gaaggtgccg
     3661 ctctggcgca gcagctggtc gaccatcgtg gtcttgccat ggtccacgtg ggcaatgatg
     3721 gcgatgttgc ggatttgttg ggtcatgtcg tactcacttc gttcatatct cggatcggtg
     3781 ctggttcagc gggggcaaga aggccttgca cctccagcgg gctcaggagc ctggttgaaa
     3841 tcagttcgcc agcggtgatg tgaccgctgc cgaggaaggc cttggcttgc ggcccgtaga
     3901 cgcgcacgcg cggcgtattc ggcagcgcga gccggcggcg cacaccgctg aggaagcggc
     3961 ccgcgccttc gtcgtcgagc cgcaccacgg gccagtcggc gatgaggctg tcggtgtcct
     4021 tcagcaacgc atcgcgctcg gcttcagtca tcgcggccag cgattccagt gtctgcgcgc
     4081 cggccagagt cagcgggccg ctgccggtgc ggcacaacgc agcaagatgc gcgccgcagc
     4141 ccagagctcg gccgatgtct tcggccagcg tgcggatgta ggtgcccttg ctgcagtgca
     4201 cttcgatcgc ccactcgtca cccttgccgt tgtcccgtcc gccatgctgc tcctgcggga
     4261 tgtcgatgcg atgaatcgtc acctggcggg gctcgcgttc aacctcgatg ccggcacgcg
     4321 cgtactcgta cagtggtttg ccgtcgcgct tcagcgctga atgcatcggt ggcacctggg
     4381 agatttcccc gacgaaacga ttgcaggcgg cctcgaaaag ctcgcgcgtg acgtgcacct
     4441 cggtgcgttc gagcacctcg ccctcggcgt cggcggtcgt cgtggtgatg cccagccgca
     4501 gcgtcgcgcg gtagatcttg tccgcgtcga gtccgacctg cgagaacttg gtggccgcac
     4561 cgaaacaaag cggtagcagc ccggtggcaa gcgggtcgag cgtgcctgtg tgaccggcct
     4621 tttcggcgcg atacaggcgc ttggccttca tcagcgcatc gttgctcgac aggcccatcg
     4681 gcttgtcgag cagcagcacg ccatggacgg cacgacgagg aatgcgcgga cgcgctgcga
     4741 atttcacgac ttcagtcttc cttcgcccgc gtcgcgttcg cctgggcgat gagcaggctc
     4801 agttcggccg cgcgctcggt cgtgcgatcg aaatggaagt gcagggtcgg gacggtgtgg
     4861 atctgcaggc gcttgaacag gccattgcgg atgaatcctg cggcctcgtt gagcgcgact
     4921 tcacactcct gcggatctcc gaccagaaca gaaaagaaga tcttggcgtg cgcatagtcg
     4981 ggcgtcactt cgaccgcgtg caaggtcacc atgccgatgc gcggatcctt cagatcgcgg
     5041 atcagcactg cgacgtcacg ctggatctgg tcggcaaccc ggaatccgcg gttgggtatg
     5101 gaacgcttgt gtttcatcgt gattctctgc agccgaatgg gcccgctgta aatgcaaacc
     5161 ccgccctctt ttgaagggcg gggttcgggg ttgggattcc ccgccgcctt acagcgttcg
     5221 tgcgacttcc ttcacttcga agaattccag ctgatcgcct tcggcgatgt cgttgtagtt
     5281 cttgagcttg ataccgcact cgaagccgga cgccacttcc ttgacgtcgt ccttcaggcg
     5341 gcgtaccgac tcgatctcgc cggtgtagac cacgacgttg tcgcgcagca ggcggaaccg
     5401 tgcgccacgg cgcaccaggc ccgcggtgac catcgagccg gccacggtgc cgatcttgga
     5461 ggcgacgaac accgtgcgga tctcggcggt gccgatgact tcctcacgct gctcaggcgc
     5521 cagcatgccg gtcatcgccg ccttgatctc gtcgaccgcg tcgtaaatga tgttgtagta
     5581 gcggatgtcg acgccgttgt gctcggccag ctttcgcgca ccggcatcgg cacgaacgtt
     5641 gaatccgatg atcacggcct tcgatgcgat ggccaggttg acgtcgctct cgctgatacc
     5701 gccgaccgcg ccgtgcacca cctgaacctt gacctctgcg gtactgagct tgagcaacga
     5761 ctgcgcgagc gcttcctgcg aaccctgcac gtcggccttg atgatcagtg gcagagactg
     5821 cgcagcacct tcgccgacgt tgtcgaacat gttctcgagc ttcgcagcct gctgcttggc
     5881 cagcttcacg tcgcggtact tgcccgagcg gaaagtggcg atttcgcgtg cgcggcgctc
     5941 atcggccagc accatgaact catcacctgc ctgaggcact tcggtcaggc cctggatctc
     6001 gacgggaatg gacggaccgg cttcagcgat cggcttgccg ttctcgtcga gcatggcccg
     6061 aacacgaccg taggtcgatc cggccagcac cacatctcca cgcttgagcg tgccggactg
     6121 caccagcacg gtagccaccg ggccgcgccc cttgtcgagc ttggcttcga tgacgaggcc
     6181 cttggccatg ctgtccttcg gcgcagtcaa ttccagcacc tcggcttgca ggagcacctg
     6241 ttccagcagg tcgtcgatgc cctgaccggt cttggctgaa accgccacga acggcgaaga
     6301 accaccgaac tcttctggca ccacctcttc ggcgaccagc tcgctcttca ggcgctcgag
     6361 gttggccccg ggcttgtcga tcttgttcat ggccacgacc agcgggacac ctgccgcctt
     6421 ggcgtgggcg atggcttcct tcgtctgcgg catcacgccg tcgtcggccg ccaccaccag
     6481 gatcaccaga tcggtggcct tggcgccgcg agcacgcatg gccgtgaagg ccgcgtgacc
     6541 cggcgtgtcg aggaaggtga tcatcccgcg cggcgtctcg acgtggtacg cgccgatgtg
     6601 ctgcgtgatc ccacccgcct cgccagccgc cacgcggctg gtccggatgt agtcgagcaa
     6661 cgacgtcttg ccgtggtcga cgtgacccat gacggtgacg accggagccc gcggcagcat
     6721 ttcgtgctcc gattcgccct cgacatcctc ctcgaggaac gcgtcgtgat catccagctt
     6781 ggcggccagc gccttgtggc ccatttcctc gacgaggatc atggccgttt cctggtcgag
     6841 ctgctggttg atggtgacca tctggcccag cttcatcaac tgcttgatga cctcggaggc
     6901 cttcacgctc atcttgtggg ccagatcggc cacgctgatg gtctcgggca cgtgcacttc
     6961 ctgcacctgc tgctcgaccg gcgcgacgaa ggtcgacacc gatccatcgc tgcgttcacc
     7021 acggcgaccg ccgcgcggcg cgcgccaacc ggcgcgggct ccaccgggtg cgtcgttgcg
     7081 cgtcttgagt ccgcgcttct tggcggcgtc gtcggcccag ctggacgaga gcttctcgga
     7141 cttgaccgac ttcttgtcgc ccggcttggc caccacggcg cccggagcac ccggtgcgac
     7201 agcgggcttg tggatggttc ccttgatgcc ttccttgcca gcctcgacgg gcttcggcgc
     7261 ctcttccggc ttcttggcgt tcagcacctt cttcggcgcc gacatcatcg cccggatggc
     7321 tgcagcctcg tcctcggcag ccttgcggcg cttggccaga tcgattgcac gctgcttgtc
     7381 ctcgtcgacc acgtccgcag ccttgatcac gcgcaacacg ggcttgaccg gctcgacgac
     7441 agccggtgga acttcggcag cagcatcggc agcgaccggc acggccgcct cggaaccagc
     7501 ggagcgggca gcggcttcga cggctgccgc ggcagcagcg gcagcggcct tctcggcctg
     7561 ggctcgggct tcagcggcct cgcgttcgag gcgcgcctgt tcatctcgct gacgctggcg
     7621 ctcggccagt tcggcctcct gcttgcgcag cagttcagcc tgagcctggg cctcatgctc
     7681 caggcgcatc aactcttcgt tgttgacttc cgccgcctct gtggccgact cgccacccgc
     7741 cggtgcgtca tcgcgcttga cgaaggttcg cttcttgcgc acctcgacct ggatggtgcg
     7801 tgccttgccg ctcgaatccg cctgcttgat ctcgctggtc gacttgcgcg tcagcgtgat
     7861 cttcttgcgt tcagcgcctg cggtgccatg ggtggcgcgc aggtagtcga gcaaccgctc
     7921 cttgtcggag tcggtcaggg catcgtccgt cgacgccttg ctgaccccgg cggactgcaa
     7981 ttgatcgagc agtgcagcgg cggggcgatt cagctcagcg gcgagttggg cgacggtggt
     8041 cacagccatt gtggattcct tgcttcgggt ctagcggtgc ggcgtgcttg cgcgatgcgg
     8101 tcggtcggct tgaagtaaat caggtggtga accagtgttc acgcgccttc atgatcagag
     8161 ccttggcctt ctcatcgtcc atgccggata tttcaatcaa ctcatcgacc gcgaggtcgg
     8221 ccagatcgtc gcgcgtgtgc acgccgccat cggccagctt cgggatcaac tcggggtcga
     8281 ggccttcgag gtcgcgcaaa tcctgagaca cgtcactgac gctctcctcg cgagcgatct
     8341 ccatcgtcag caaggcatcc ttggcacgtg tgcgcaactc gttgacggtg tcctcgtcga
     8401 agccctcgat ctcgagcatt tcctgcatcg gaacgtaggc cacttcctcg aggctggaga
     8461 agccctctgc gatcaggatg tcggcgacct cggcatcgac gtcgagcttc tcgacgaaga
     8521 gcttgcgcag gctgtcgctc tcgaccgcct gcttctccga agactcttcg gcggtcatga
     8581 tgttgatgcg ccagccggtc atctccgagg ccaggcggac gttctgccct ccacgcccga
     8641 tggcgatggc gaggttttcc tcgtcgacga ccacgtccat cgcgtgacgc tcttcgtcga
     8701 ccacgatcga ctgcacgttg gcaggcgcca gtgcaccgat gacgaactgc gcggggtcct
     8761 cggaccacag cacgatatcg acgcgttcac ctgccagctc gttggtcaca ccgttgacgc
     8821 gcgaaccgcg cacgccgacg caggtgccga tcgggtcgac acgcttgtcg tgcgactgca
     8881 cggcgatctt ggcgcgtgag cccggatcgc gcgcacagga cttgatctcg agcaggccct
     8941 gttcgatctc gggaacttcc tgcgcgaaca gttcgatcat gaacccgggc gcgctgcgcg
     9001 acaggatgat ctggggcccg cgcaaggtcg agtcgacctc gaggatgtaa gcacgaaccc
     9061 ggtcaccgat gcgcaggttt tccttcggga tcatctcgct gcggcgcagg cgcccttcga
     9121 cgcgtccgga ctcgacgatc acgtcgccct tgtccaggcg cttgacggtg ccgacgaaga
     9181 tcttctcgcc ccgcgaaagg aagtcgttga gcagctgttc gcgctcggcg tcgcggatct
     9241 tctgcaggat gacctgcttg gccgcctgtg cgccgatgcg accgatcgat accgactcga
     9301 ccggctcctc gatgtagtca tcgacctcga tgtcggggat ctgctccagc gcctcgaagt
     9361 gcaggatttc ggagtccggc agttgcagac ccgcatcgtc agcgaccacg tgccagcgcc
     9421 ggaaggtttc atacgcaccc gtatcgcgat ccacggtcac gcgcatgtcg acctcaccgc
     9481 cgtggagctt cttggatgcc gacgccagcg ccgcttcgac agcgccgaag acgacttcgc
     9541 ggtccacgct cttctcgcgc gaaatggcat ccaccagcat caacagttcg cgattcatcg
     9601 tctgcggtct ccatccatct cgttagcggc cgactcgacc tgctcggtcg gcacctcgac
     9661 aggcgccgca aaccgacgcc ccttgaaatc aaccaccggc accagccggg cttctcgcac
     9721 ttcgtcgaac gcaaaatcca gcgcctgatc gacgccgtcg ccctggaaga cgagacgcca
     9781 gccgtcgccc agcggctcca gcacgccctg gaaatgcttg cggcccttga aggccagctt
     9841 cagcgccaga tcgactcgct gcccgctgaa ccgcaggaaa tcagccggct tcttcaacgg
     9901 ccggtcgagt cccggggacg acacctccag ccgctcgtac gcacaggctt cgacctcgag
     9961 cacgtgctgc aactggcgcg tgacgcgctc gcaatcttcc acggtgatga actcgctgcc
    10021 cgcgggcgtc gagcctggcg agacaggtgc aggcttgtcg atgtagacgc gcagcagccc
    10081 cttcgggctg cgctcggtgt ccaccaagtc atatcccagt ccggtcaccg tcttctcgat
    10141 ggcaagttgc cagttcatcc ccgcttgatt ccccgtctgt cttcgcgcgc cgcctgtcgg
    10201 aaaacccgac cggcgacctg catgaaatag ccgatcgaac aaaaaaaatg ggctggaaaa
    10261 gtccgcccat ggtgtcttct ctgtctactc ggaaaaccgg aattgtacct tgcaggggtg
    10321 ctaccggcaa cacccctgac ctgtcaagtc tgggtagtcg ccggcgatcc aggcgcagcg
    10381 ccggcaaagc gacacccgcc ccttcgagtc accagttggc ctcgcgctcc ggcgtggcac
    10441 cgatgcgatg gatggacagg tccgccccct cgtactcttc ctcgagcgtc agccgcagcg
    10501 aagtgacggc cttcagtcca ccgtagacga tgacaccggc aatcagcgcc cacgacacac
    10561 ccagcagcgt gccgacgacc tgcgctccca ggctcacgcc tcccacacca cccagcgact
    10621 ggctgccgaa gatgccgcag gcgatgccgc cccaagcccc gcacaagccg tgcagaggcc
    10681 acacgccgag cacgtcgtcg atcttccagc ggttctgcgt cagggtgaac atcgacacga
    10741 agatggcact tgccacgcag cccaccacga gggcgccagc agggtgcatc acgtcggagc
    10801 cggcgcacac ggccaccaga ccggccagcg gtccgttgta gacgaacccc ggatcgttgc
    10861 gccccatcag caccgcgacc agcgtgccgc cagccatcgc catcaacgaa ttgaccgcga
    10921 ccagcccgga gatcttctcg atggtctggg cgctcatcac attgaatccg aaccagccca
    10981 ccgccagcac ccaggcgccg agagccagga acgggatgct cgacggcgga tgcgcgctga
    11041 tgccgccgtc ctttcggtag cggttgctgc gcgcgccgag catcagcacg gcgaccagtc
    11101 caacccagcc accgaaggcg tgcaccacca cgctgccggc gaagtcgtgg aactcagcgc
    11161 cggtcagcgc cttgatctgg ccctgcagtc cgaacttgcc gccccagacc accccttcca
    11221 gcagaggata ggccgcaccc accagcaacg ccgtggcgat cagttgcggc ccgaatcgcg
    11281 cccgctcggc gatgccgccc gacacgatgg ccggaatggc agcagcaaag gtcagcagga
    11341 agaagaagcg aaccagctcg aagccattct tctccatcag cgtctcggca ctgcccagga
    11401 agccgatgcc gtacgccacg gtgtagccga tgaagaagta catcacggtc gacaccgaga
    11461 agtcgaccag gatcttgacc agcgcattga cctggttctt cttgcgcacg gtgccgagtt
    11521 cgaggaatgc gaaccccgcg tgcatcgcca gcaccatgat ggcgcccaac aagatgaaga
    11581 atgcgtctgt gccctgcttg acctgatcca aggtgaactc caacgggtgc tgtgcatcgg
    11641 tgcgaaatgc accgacaaac caaaaaacgc accaatgaag caagatttat tccctgattg
    11701 gtgcggacaa ttgcaccaat cagccgcaaa ttcagcagcc ccagccgcct caaatcgtcg
    11761 agccgcacct catcagtgcc tgacgcacct tttcgcgcat caaggcagct cgggcgcaat
    11821 ctcggtggcg tgatccacca gcgcaccgag gatgtcctgg gcgcgctcat cgccgctgtc
    11881 ctcgaactgg ctgctcagtt gatcgatgcg ctcgaagaac gcttccagcg tcaagccgcc
    11941 cccctcaccg ccgtgcaggc ggcgcaggcg gagttcgagc cactgctggg cttcggcgcc
    12001 gctcagggtg tccgggaagt tgcgcgcgcg gtagcggaac agcagctcac cgagtcgatc
    12061 gtcggcaaac acggggcgct gcccggcaag agcctgcgga tcgagtgcgc gcagccgttg
    12121 caacaggcgc cgatcgtcat tgccgacgaa ccccccgtaa aggtcttcat cgacatcggg
    12181 tgtccctgtg gtcgccggac gttcgaacac atctggccac agcccggcga gcaagggcgc
    12241 ctgatgggcc gcgacctcag cgtgcctgca cgcctggacc acatcgacgc cccagcgcgt
    12301 ggccatctcg gcgctgagcg tcttcaggtt ggcgatgacc accggcgact tgttgaggtg
    12361 gatggtcttg atcggcagtc gcgtcacgcc ctccggcagc gcatcggctc gggaaaacat
    12421 ccgctggcga atggcgtcgg catccagcgc acgaagcacg cccggatctt ctgccaggtc
    12481 ccagacgatg acctcgttct tgttcgtcgg atggggcgcc agaggccaca ccagcgcgat
    12541 gcaaccgcgg tcgggcgggt acatgccgga cacgtgcagg aacggcctgc cgtggttctg
    12601 ggcgctggcc atctcctcga tgaccgcgtc cttcttgcgc agccgcaggc agaagtccca
    12661 cagtttcggc tgccggtcct tcaggagtcg ggcgagcgcg atggtggcgc gcacatcgga
    12721 cagcgcgtcg tgcgcggcct cgtgcgccag gccgttggcc gcagtcaggt gttcgagctt
    12781 gaacgacggc cggccgtcgt catgccgggg ccaggcgatg ccatccggcc gcaaggcgta
    12841 ggcgcagcgc accacgtcca ggaggtccca gcgcccgcac tggttctgcc attcgcgagc
    12901 gtacggatcg atcaggttgc gccagaacag gaagcgtgtg acctcgtcgt cgaagcggat
    12961 cgagttgtat ccgaccccca cggtgccggg cctggcgagc tgcgcctcga tcgccgcggc
    13021 aaatgcgtgc tcgggcacgc cgcgctcggc gcagatctgc ggcgtgatgc cggtcagcag
    13081 gcagctctcg gggtccggca ggcagtcgag cgggggctgg cagtacagcg tgacgggctc
    13141 gtcgatctcc tggagatcgg catcggtgcg aacaccggcg aactgcgccg gacggtcgcg
    13201 gcgcggcacc acgccgaagg tttcatagtc atgccagaag aagctgaacg ggtccatgga
    13261 ctgcaacata ccacccgtcc acaatggttc gcatcacccc cggggagccg ccatgaatcg
    13321 ttcgaccgcc agaagcaagt cgcccttcgc gacgaagcac gccttcgcga agactctgaa
    13381 gaccttcgtc accgacagcg gcatcgaggg tggctactac tcgctgcccg cactggcgaa
    13441 gatcatcccg aacgtcgcgc ggctgccggt gtcgctgcgc atcgtgctgg aaagcgtgct
    13501 gcgcaactgc gacggcagga aggtgacacc cgagcacgtc gagcggctcg cggcctggaa
    13561 gcccaacgca ggccgaaccg acgagatccc attcgtcgtc gctcgcgtgg ttctgcagga
    13621 cttcaccggg gtgccgctgc tggccgatct ggccgccatg cgcaatgtcg ccgcggcgat
    13681 gggcaaggac ccaaagacca tcgagccgct ggtgccggtc gacctggtcg tcgatcacag
    13741 cgtgatgatc gatcactacg gcaacaggaa ggcgctcgac ctgaacatgc ggctcgagtt
    13801 ccagcgcaac cgcgagcgct acgagttcat gaagtggggc atgcaggcat tcaacacgtt
    13861 cggcgtcgtg ccgccgggct tcggcatcgt ccaccaggtc aacctcgagt acctggcgcg
    13921 cggcgtgcac aagacaccga agggccagcc gaacgcgggc gtctattacc ccgactcgct
    13981 ggtcggcacc gacagccaca cgaccatgat caatgggatc ggcgtggtcg gctggggcgt
    14041 tggcggcatc gaggccgagg ccggcatgct gggccagcct gtgtatttcc tgacgcccga
    14101 tgtggtcggc ttcgaactga ccggccggct gcgcgaaggg gtcaccgcaa ccgacctggt
    14161 tctgacggtg acggagatcc tgcgccaggc caaggtggtc gggaaattcg tcgagttctt
    14221 cggcgaggga acggccagcc tggatctgcc tgaccgcgcg acgatcgcca acatggcgcc
    14281 ggagtacggc gcgacgatgg gcttctttcc ggtcgacgaa aagaccatcg actatttcag
    14341 gggaaccggc cgcacgaagc gggaaatcga ggctttcgag gcctacttcc gtgcgcagaa
    14401 gctcttcggc gttccgaagc gtggtgacat cgactacagc gcggtggtga cactcgacct
    14461 gggccgcgtg gcaccgagcc tcgccggtcc gaagcggccg caggaccgca tcgaactcgg
    14521 caacgtgaag gcccagttca gttcgctgta cagccagccc ccgactcaga acggattcaa
    14581 ccagccggca gggagccttt acttccggca cctggcgcgc tccagcaacg ccgcccgcgg
    14641 tactccggcc acggcaccgg ccttgattcc cggggcggcc cgcgatgtcg tcgagatggt
    14701 cgggaaccgc tccacgctcg aagcatcgct gtccgaatca gaaccggcgc acccggtgga
    14761 tggggacatc tcgatcggca acggcgacgt gctgatcgcg gccatcacct cctgcaccaa
    14821 cacctcgaac ccgagcgttc tgctggcagc gggcctgctg gcgaagaagg cggtcgaggc
    14881 cgggctgaag gtgcagccgc acatcaagac ctcgctcgca cccggttcgc gcatcgtgac
    14941 cgagtacctg gagaaggccg ggctgctgcc ctacctggaa aagctcggct tctatctggc
    15001 tggctatggc tgcacgacct gcatcgggaa cgccggcgat ctgcgacctg agttcaacga
    15061 cgtgatcacc gagaacaacc tggtgtgcgc ggcggtgctg tcgggcaacc gcaatttcga
    15121 ggcgcgcatc cacccgaacc tgaaggcca
//
LOCUS       DAOORV010000087        14871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4923_length_14871_cov_7.981911, whole genome shotgun sequence.
ACCESSION   DAOORV010000087 DAOORV010000000
VERSION     DAOORV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14871
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4923_length_14871_cov_7.981911"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKV56_12050"
     CDS             complement(<1..1040)
                     /locus_tag="PKV56_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOX68591.1"
                     /translation="MTAPAVTPAFPSFTARPVPAAMLDALKVRFGDRCSTACAVREQH
                     GRDESPFDVPPPEAVIFCESTDDVAAVVALAYLHEVPVIPYGTGSSLEGHLLAVQGGV
                     SVDLGRMKAVLAVEPDDLTVTVQAGVTRMQLNDEIRHTGLFFPVDPGADASLGGMAAT
                     RASGTNAVRYGSMRENVLGLTVVTAQGQVIRTGTRARKSSAGYDLTRLMVGSEGTLGV
                     ITEITLKLHPQPEALSAATCSFPTVDAAVRTTIQVIQMGVPIARCELLDAHAVRAVNR
                     HDRLTLPEAPMLLMEFHGTEASVQEQASTVQAVAADFGGEHFQWATTPEQRTKLWAAR
                     HHAYLAGLQTKSA"
     gene            1166..1747
                     /locus_tag="PKV56_12055"
     CDS             1166..1747
                     /locus_tag="PKV56_12055"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOX68592.1"
                     /translation="MSNRLTQIATRTGDDGTTGLGDGKRVSKDNPRVHAMGDVDELNS
                     QLGVLLAEPLPDDVRELLVVIQHELFNLGGELSIPGYELLKTQAVIQLDEALARYNDT
                     LPKLKEFILPAGTRSAALAHVARTIARRAERAVVALAAAESVRDEPRHYLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWKSERLQRDAAAD"
     gene            complement(1772..2887)
                     /locus_tag="PKV56_12060"
     CDS             complement(1772..2887)
                     /locus_tag="PKV56_12060"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOX68593.1"
                     /translation="MSHQNAANEGRRALSEKTSQTDDERIRDVTPLPPPEHLIRFFPI
                     RGTPIEALVGDTRKRIRRIMNGKDDRLLVVVGPCSIHDPAAAIEYARKLKEQRDLHAD
                     TLEIVMRVYFEKPRTTVGWKGLINDPYLDESYRIDEGLRIARQLLVEINRLGVPAGSE
                     FLDTISPQYIGDLISWGAIGARTTESQVHRELSSGLSAPIGFKNGTDGNIRIATDAIQ
                     AAARPHHFLSVHKNGQVAIVETTGNPDCHLILRGGKEPNYDAAHVEAACKDLEAAKLP
                     PTLMVDCSHANSSKQHDKQNDVARDIAAQLSAGSRRVFGVMVESHLSAGAQKFTPGKD
                     DPAKLSYGQSITDACIGWDDTLTVLDTLSQAVKARRR"
     gene            complement(3116..4591)
                     /gene="tldD"
                     /locus_tag="PKV56_12065"
     CDS             complement(3116..4591)
                     /gene="tldD"
                     /locus_tag="PKV56_12065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HOX68594.1"
                     /translation="MISREPTIERLGIAQALLIEPFGLTEATLTRALQAIATHRIDDA
                     DLYFQYTRSEGWSLEEGIVKSGSFGIDQGVGVRAVAGEKTAFAYSDDISEAALMDAAQ
                     TVRTIAAAGQSRRVKINTTRKVAGSRVLYAPMDPIASLDSTQKVALLERVERLARAKD
                     PRIKQVMASIAGEYDVVLVARADGTRAADVRPLVRLSVTVIAEQTINGEVRRETGSSG
                     GGGRFGYGYFHDAMIESYVNDAVGAALTNLESRPAKAGEMSVVLGSGWPGVLLHEAIG
                     HGLEGDFNRKGSSAFSGMIGQRVAAKGVTVLDDGTLPDRRGSLNIDDEGNPSQRNVLI
                     EDGILKGYLQDSMNARLMGVKPTGNGRRESYAAVPMPRMTNTYMLSGDKTKEEIIAGL
                     KRGLYASNFGGGQVDITSGKFVFSASEAFWVENGKIQYPVKGATIIGNGPDALTRVSM
                     IGNDMKLDTGVGTCGKEGQSVPVGVGQPTLRLDGLTVGGTA"
     gene            complement(4705..5619)
                     /gene="rsmI"
                     /locus_tag="PKV56_12070"
     CDS             complement(4705..5619)
                     /gene="rsmI"
                     /locus_tag="PKV56_12070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOX68595.1"
                     /translation="MTTPIASLLLQAASAAAGSQQYPAAALYVVATPIGNLADLTLRA
                     IHVLSLVDAVACEDTRHTAPLLRHLGLDKPLLAVHQHNEQSSSGDVIARLARGERVAY
                     VSDAGTPAVSDPGAALVAAVVASGRAVVAIPGASSAITAVSVAGDTVGSGFRFIGFLP
                     ARGNERALALKALAVAVETQVLFEAPHRIEALVSGLAEACGARIVTLCRELTKQFETV
                     ATLPAAALPGWLAGDANRLRGEFVLVVHAMAPSESADETVRHDVVLQTLLAELPLKQA
                     VSLAAALSGAPRNALYARALELKGRAAD"
     gene            5606..5980
                     /locus_tag="PKV56_12075"
     CDS             5606..5980
                     /locus_tag="PKV56_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOX68596.1"
                     /translation="MGVVTTKTVGDGGEARALAHLLAHGLTLVQRNYRVAAGPRARGG
                     EIDLILRDREGTLVFVEVKTRKTDGHGGAAASVSETKQRSLIFAAQHFLRSWASPPAC
                     RFDVVAIEADRIQWLQAAFDAS"
     gene            6058..6648
                     /locus_tag="PKV56_12080"
     CDS             6058..6648
                     /locus_tag="PKV56_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HOX68597.1"
                     /translation="MIEQRIQQQFFDSADLMYAAAEVLSKPLAEAVEALVGCITAGGK
                     VLVCGNGGSASDAQHFAAEFVGRFERERPGLAALALTTDTSILTAIGNDYDFNSIYSK
                     QVQALGNPGDVLIAISTSGNSGNVLAAVAAAHAKDMTVVALTGRSGGKLGALLTDTDV
                     HICVPHDRTARIQEVHGLALHCLCDAVDQQLLGEQE"
     gene            6654..7268
                     /locus_tag="PKV56_12085"
     CDS             6654..7268
                     /locus_tag="PKV56_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOX68598.1"
                     /translation="MSKLALSLVAAATVSTALTGCFPLVLGGAVVGGTSVYTDRRTSG
                     TQLEDEAIEIKALSRNSEAIGSRGRVSVTSYNRLALITGEVTSDADRALIEKTVSRVE
                     NVRSIVNELAVVKELPTSNAGTDAIVTGKIKASLVDSKDVFAQAVKVVTERGTVYLMG
                     RVTEREAARAAEIARGVSGVQKVVRVFEIISEAELANLNAPASK"
     gene            complement(7293..8195)
                     /locus_tag="PKV56_12090"
     CDS             complement(7293..8195)
                     /locus_tag="PKV56_12090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX68599.1"
                     /translation="MADLGVRSYESIEPQRVAFLGLGVMGHPMAGHLARAGHHVTVYN
                     RTKAKADAWVGEFGGTVATTPRAAAEGAGFVFACVGNDDDLRAVVLGEGGAFAGMQTG
                     AVFVDHTTASAAVARELCAVATTLGLSFIDAPVSGGNLGAINGSLTVMCGGDAAAFSR
                     AQPVALAFAKAMTLLGDSGSGQLAKMVNQIAIAGLVQGLAEAIAFGDQAGLDMKTVLG
                     VIGKGAAQSWQMDNRGPTMVDGKFDFGFAVDWMRKDLGLVLDEAKRNGARLPVTALVD
                     QFYADVQAAGGQRWDTSSLITRLR"
     gene            complement(8197..9333)
                     /locus_tag="PKV56_12095"
     CDS             complement(8197..9333)
                     /locus_tag="PKV56_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOX68600.1"
                     /translation="MERDQASKFVNDLLRLMVSRNGSDLFLTSDFPPAIKVDGKVTKV
                     SPQPLTGQHTMALARAVMNDKQAGEFERTKECNFAISPQGIGRFRVNAFVQQGHIGMV
                     MRTIPQTLPTIDSLNLPKVLKDVSMTKRGLVIFVGATGSGKSTSLAAMVDFRNENSFG
                     HIITIEDPVEFVHPHKNCIVTQREVGIDTDGWEQALKNTLRQAPDVILMGEIRDRETM
                     EHAVAFAETGHLCMATLHANSANQALDRIINFFPEERRAQLLMDLSLNLKGLVSQRLL
                     PRQEGKGRVAAVEIMLNSPLIADLIFKGEVAEIKEIMKRSREHGMQTFDQALFDLYES
                     RAVTYEDAIRNADSVNDLRLQIKLNSQRARNTDLAAGTEHMTIV"
     gene            complement(9478..10521)
                     /locus_tag="PKV56_12100"
     CDS             complement(9478..10521)
                     /locus_tag="PKV56_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOX68601.1"
                     /translation="MDITQLLAFSVKNKASDLHLSSGLPPMIRVNGDVRRINVDPLEH
                     KDVHSMIYDIMNDSQRKAYEEVLECDFSFEIQGLARFRVNAFNHNRGAGAVFRTIPSK
                     ILTLEQLAAPKIFAELALKPRGMVLVTGPTGSGKSTTLAAMVNHLNENEYGHVLTVED
                     PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRAMLSESLIGVISQTLCKTKDGTG
                     RVAAHEIMLGTAAIRNLIRESKIAQMYSAIQTGNSVGMQTLDQNLADLVRRNVVSPHE
                     ARAKAKFPENFPG"
     gene            10614..11300
                     /locus_tag="PKV56_12105"
     CDS             10614..11300
                     /locus_tag="PKV56_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOX68602.1"
                     /translation="MATIVQNLQKVHQRIAQACAAAQRPVQSVTLLVVGKTFAADTVR
                     AAFDAGERCFGENYVQEGIDKIAALADLREQIQWHLIGPLQGNKTRVVAESFDWVHSV
                     DRLKIAQRLSEQRPAHLPPLQLCLQVNISGESSKSGLTIDELPEVARAVAALPRVRLR
                     GLMAIPAPAVDLAAQRAPHRRLRELLSLLQGCGLDVDTLSMGMTADLEAAIAEGATIV
                     RVGTAIFGAR"
     gene            11461..12486
                     /locus_tag="PKV56_12110"
     CDS             11461..12486
                     /locus_tag="PKV56_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOX68603.1"
                     /translation="MQSSPRSGTTVVRKTITSLGIGAAILALGGCVSTAPVVGGGEKG
                     PVTGSAGGATTANANSSLEHCDQTLGTLGVVEDQAASWFQELRNYKLGSTVPVIRMMI
                     QQSNCFIVVERGNAMQNLNTERALQQSGEMRGGSNFGKGQMVAADYTMSPSIQFSQNT
                     GGVGGALGGFSGALGVAGAIAGGMKTNEAATTLILIDNRSGVQLAAAEGSAKNMDFNV
                     MGGLFGGGVGGGAGGYTNSPEGKIIIAAFADSYNQLVRAVRNYKAQTVKGGLGTGGAL
                     GVSGGSTAASKAGDAAAAAPAAKSTTKTPAAATATKPATTTKPAAKKPAAKPATTTTT
                     TTPAKTQ"
     gene            12506..13321
                     /gene="proC"
                     /locus_tag="PKV56_12115"
     CDS             12506..13321
                     /gene="proC"
                     /locus_tag="PKV56_12115"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOX68604.1"
                     /translation="MTLSSIAFVGGGNMAGALIGGLLRSGWSAEAVAVVEPSAAQRET
                     LASRFGVRTLAEADATLGRADCVVWAVKPQLFKAASAPCAAHVGGALQLSVMAGIRSD
                     AIALATSSDRVVRAMPNTPALIGEGMSGLYARTAVSAEERTQVEKLLAPTGQTLWVDR
                     EEQLDAVTAMSGSGPAYVFYLLEAWIEAGTAMGLSAAQARQLAMATLAGATSLARDSD
                     ETPAMLRERVTSKGGTTHAAITSLEADGVRAAFVRALKAAEQRAGELGDEFGR"
     gene            13405..14676
                     /locus_tag="PKV56_12120"
     CDS             13405..14676
                     /locus_tag="PKV56_12120"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOX68605.1"
                     /translation="MSNLSYLNPTAASPWQTYLSQVDRVIPHLGHLARWADTLKRPKR
                     ALIVDVPIEMDDGSVRHFEGFRVQHNLSRGPGKGGVRYHPDVTLEEVTALAAWMTIKC
                     AGVGLPFGGAKGGIRVDPKQLSMKELERMTRRYTSEIGIIIGPQRDIPAPDVNTNAQI
                     MAWMMDTYSANTGATATGVVTGKPIHLGGSLGRVKATGRGVFVTGREAARRIGLNLDG
                     ARVAVQGMGNVGSAAAELFVQAGATLVAVQDHTGTLVNPNGFDMAETAAVLKRDHGIG
                     NYADAERIDNEDFWAVDCDILIPAALEGQITAERAERIKARLVLEGANGPTLPEADDV
                     MKARGILVVPDVICNAGGVTVSYFEWVQDFSSFFWSEDEINLRLDKIMVDALKHIWET
                     ADRHRISLRTATFAVACERILTAREERGLYP"
BASE COUNT         2554 a         4964 c         4886 g         2467 t
ORIGIN      
        1 gcggacttcg tctgcagacc tgccaggtag gcgtgatggc gcgccgccca cagcttcgtg
       61 cgctgctccg gggtcgtcgc ccactggaag tgctcgccgc cgaagtccgc ggcgacggcc
      121 tgcaccgtcg aggcctgctc ctgcacgctg gcctcggtgc catggaattc catcagcagc
      181 atcggtgcct cgggcagggt cagtcggtcg tgccggttga cggcccgcac cgcgtgcgca
      241 tcgagcagtt cgcagcgcgc gatcggcacg cccatctgga tgacctggat cgtggtgcgc
      301 acggccgcgt cgaccgtcgg aaagctgcag gtggctgccg acaaggcctc gggttgcggg
      361 tggagcttca gcgtgatctc ggtgatcaca ccgagcgtgc cctcgctgcc gaccatcagc
      421 cgcgtcaggt catagccggc cgacgacttc cgggcgcgcg taccggtgcg gatgacctgc
      481 ccctgcgccg tcacgacggt caacccgagc acgttctcgc gcatcgagcc gtaacgcacc
      541 gcgtttgtgc cgctggcgcg cgtggcggcc atcccgccga gactcgcatc ggcacccgga
      601 tcgaccggaa agaacaggcc ggtgtgacgg atctcgtcgt tcagttgcat ccgcgtcacg
      661 cccgcctgca cggtgaccgt caggtcgtcc ggctcgaccg ccaggaccgc cttcatgcgc
      721 ccgagatcga cgctgacacc gccctgcacc gccagcaggt gcccctcgag cgatgaaccc
      781 gtgccgtatg gaatcacagg cacctcgtgc aaataggcca gtgccacgac agcggccacg
      841 tcgtcggtgc tctcgcagaa gatcacggcc tcgggcggcg gcacgtcgaa cggcgactcg
      901 tcccggccat gctgctcgcg cacggcgcag gcggtcgaac accggtcgcc gaaacgcacc
      961 ttcagtgcat caagcatcgc agcgggcacc ggacgggccg tgaacgacgg gaaagcagga
     1021 gtcacagcag gtgcagtcat cgtgaacgga tcaggcggcg gaagccgcca ttgttcatcg
     1081 cgcacgccgg accgggtgtt cgggcatcat ccagccccga tccggccgcg ggcgccggca
     1141 gcaacacact gacaggcaca accccatgtc caaccgactc actcagatcg ccacccgcac
     1201 cggggacgac ggcaccaccg gtcttggaga cggcaagcgg gtatcgaagg acaacccgcg
     1261 cgtgcatgcg atgggcgatg tcgacgagct caactcgcaa ctcggcgtgc tgctcgccga
     1321 gccgctgccc gacgacgtgc gcgaactgct ggtggtgatc cagcacgagc tgttcaatct
     1381 gggtggagag ctgtcgatcc cgggctacga gttgctgaaa acgcaggccg tgattcaact
     1441 tgatgaggcc ctggcccgct acaacgacac gttgcccaag ctgaaggagt tcatcctgcc
     1501 agccggcacc cgcagtgccg cgctggcgca cgtggcgcgc accatcgccc ggcgcgccga
     1561 acgggcggtg gtggccctgg ccgccgccga atccgtgcga gacgaaccac ggcactacct
     1621 gaaccgcctg agcgacctgc tgttcgtgct ggcgcgcgtc ctcaatcgcg ccaacctgga
     1681 cggtctgggc ggcgacgacg tctactggaa aagcgaacgg ctgcagcggg atgccgccgc
     1741 cgattgaccg gcgacatctc gcttcggtgc gtcagcgacg ccgcgccttg acggcctgac
     1801 tgagtgtgtc cagcaccgtc agggtgtcgt cccagccgat gcaggcatcg gtgatgctct
     1861 ggccgtagga cagcttggcc ggatcgtcct tgcccggggt gaacttctgc gcgccggcgc
     1921 tcaggtggct ctcgaccatc acgccgaaga cgcggcggct gcctgcggaa agctgcgccg
     1981 cgatatcgcg cgccacgtcg ttctgcttgt cgtgctgctt gctgctgttg gcatgcgagc
     2041 agtcgaccat cagcgtcggc ggcagcttgg ccgcttcgag gtccttgcaa gccgcctcga
     2101 catgcgccgc gtcgtagttc ggctccttgc cgccgcgcag gatcaggtgg cagtccgggt
     2161 tgccggtggt ctcgacaatc gcgacctggc cgttcttgtg aacgctcagg aagtggtgcg
     2221 gtcgcgcggc agcctggatc gcatcggtcg cgatgcggat gttgccgtcg gtgccgttct
     2281 tgaagccgat cggcgccgac aggcccgatg acagctcgcg atgaacctgg ctctcggtgg
     2341 tgcgtgcgcc gatcgcaccc cagctgatca ggtcaccgat gtactgcggg ctgatggtgt
     2401 cgaggaattc gctgcccgcg ggcacgccga gccgattgat ctcgaccagc aactggcgcg
     2461 caatgcgcag cccctcgtcg atgcggtagc tctcgtccag gtaggggtcg ttgatgaggc
     2521 ccttccagcc gacggtggtg cggggcttct cgaagtacac gcgcatcacg atctcgagcg
     2581 tgtcggcatg gaggtcgcgc tgctccttga gctttcgcgc gtactcgatg gccgccgccg
     2641 gatcgtggat cgagcaaggt cccaccacga ccagcaaccg atcgtccttg ccgttcatga
     2701 tgcggcggat gcgcttgcgg gtgtctccca ccagcgcctc gatcggcgtg ccacggatcg
     2761 ggaagaaccg gatcaagtgc tcgggcggcg gcagcggcgt gacgtcgcga atccgttcgt
     2821 cgtcggtctg gctggtcttt tcgctgagcg cgcgacggcc ttcgttggca gcgttctggt
     2881 gggacatggg gggctccagg gttcgggtgg atatctgctc gtgatggaga aaagcctgcc
     2941 ttgccgaaaa aaaaccgccg ggggttgccc ggcggttttg agattcggtt tgcgtttcgg
     3001 tctgtctacg cgcttgcctc tcgtccgccg gtgcggtttg agaaccagaa atagaagaaa
     3061 ccaaaaaagt gcttcattgc ctgggcaatg taacacgcgc gcctcgtacc agacctcagg
     3121 cggtgccgcc caccgtcagc ccatcgaggc gcagcgtggg ctggcccacg cccaccggga
     3181 cgctctggcc ctccttgccg caggtgccga cgccggtgtc gagcttcatg tcgttgccga
     3241 tcatgctgac gcgggtcagt gcgtccgggc cgttgccgat gatcgtggcg cccttgaccg
     3301 ggtactggat cttgccgttc tcgacccaga acgcttcgct ggccgagaag acgaacttcc
     3361 cgctggtgat gtcgacctgg cccccgccga agttggatgc atacaagcca cgcttcagtc
     3421 cggcgatgat ctcctctttc gtcttgtcgc cactgagcat gtaggtgttg gtcatgcgag
     3481 gcatcggcac ggcggcgtag ctctcgcgtc gaccgttgcc tgtcggcttg acgcccatca
     3541 gccgcgcatt catcgaatcc tggaggtaac ccttcaggat gccgtcctcg atcagcacgt
     3601 tgcgctggct cggattgcct tcgtcatcga tgttcagcga gccgcgccgg tccggcagcg
     3661 tgccgtcgtc gagcacggtg actcccttgg ccgcaacgcg ctggccgatc atgccgctga
     3721 aggcactgga acccttgcgg ttgaaatcgc cttcgagccc gtggccgatc gcctcgtgca
     3781 gcagcacgcc cggccagccg gaacccagga caacgctcat ctcgcccgcc ttggccggac
     3841 gggactcgag attggtcagc gccgcgccca ccgcgtcgtt cacgtagctt tcgatcatcg
     3901 cgtcgtggaa gtagccgtag ccgaagcggc cgccgccgcc gctgctgccg gtctcgcggc
     3961 gcacttcgcc gttgatcgtc tgctcggcaa tgaccgtcac gctcagacgc accagcggcc
     4021 gcacgtcggc ggcgcgcgtg ccgtcggcac gcgccaccag caccacgtcg tactcgccgg
     4081 cgatgctcgc catcacctgc ttgatgcgcg gatccttggc acgggccagc cgctctacgc
     4141 gttccagcaa ggcgaccttc tgcgtgctgt cgagcgaagc gatcgggtcc atcggcgcat
     4201 acagcacccg actgccggcc accttgcgcg tcgtgttgat cttgacccgc cggctctgcc
     4261 cagccgcagc gatcgtgcgc accgtctgcg ccgcatccat cagggccgct tcggagatgt
     4321 catccgaata cgcgaatgcc gtcttctcac ccgccaccgc acgcacaccg acgccctgat
     4381 cgatgccgaa gctgccgctc ttgacgatcc cttcctcgag gctccagcct tcgctgcgcg
     4441 tgtactggaa atacaggtcg gcgtcgtcga tgcggtgcgt ggcgatcgcc tgcagcgcac
     4501 gggtcagcgt cgcttcagtc agaccaaagg gctcgatcag caaagcctgg gcgatgccca
     4561 gacgttcgat ggtgggttcg cgcgaaatca tcggtggtct ttcaaagctg gaaatgattg
     4621 tagaagtggg tctgcagaag tactttcaaa gggcaaacat ccacgccctc cgatcagctc
     4681 gcggactgcc ccatgtcgcg cgcatcaatc agcggcgcgg cccttcagtt ccagagcgcg
     4741 cgcgtaaagc gcattgcgcg gagcgccact gagcgcggca gccagcgaca ccgcttgctt
     4801 cagcggaagc tcggccagca gcgtttgcaa cacaacgtcg tggcgaaccg tttcgtcggc
     4861 cgactcgctg ggggccatcg cgtgcacgac cagcacgaac tcgccgcgca gccggttcgc
     4921 gtcgcccgcc agccagccag gcagcgccgc tgccggcaat gtcgccacgg tctcgaactg
     4981 cttggtcaat tcgcggcaga gcgtcacgat tcgcgcacca caggcttcgg ccaggcccga
     5041 aaccagcgcc tcgatgcgat gtggcgcctc gaaaagaacc tgggtctcca cagccacggc
     5101 cagcgccttc aacgcaagcg cacgctcgtt tcccctggcg ggcaggaagc cgatgaatcg
     5161 aaacccgctg ccgacggtgt ctcccgccac gctgaccgcc gtgatcgcgc tgctggcccc
     5221 gggtatcgcc acgacggcgc gccccgaggc cacgaccgcg gccaccagag ccgcaccggg
     5281 atcgctcacc gccggggtgc ctgcatcgct gacataggcg acgcgctcgc cacgcgccag
     5341 ccgtgcgatg acgtctccgg aagacgactg ctcgttgtgc tggtgcaccg cgagcagcgg
     5401 cttgtcgagg ccgaggtggc gcaacagcgg tgcagtgtga cgagtgtcct cgcaggccac
     5461 cgcatcgacc agcgaaagca cgtggatcgc gcgcagcgtc aggtcggcca ggttgccgat
     5521 cggcgtggcg acgacgtaca gggcggcggc aggatactgc tggctgccgg cagcggctga
     5581 agctgcctgg agcaacaagg aggcgatggg tgtggtcacg acgaagacgg tgggagatgg
     5641 aggcgaagcc cgcgcgctgg cgcatcttct ggcccacggg ctcacgctcg tgcagcgcaa
     5701 ttatcgggtc gcagccggac ctcgtgcccg tggcggggaa atcgatctga tcctgcgcga
     5761 ccgcgaaggc acgctggtgt tcgtcgaggt caagacccgc aagaccgatg gacacggcgg
     5821 ggccgcagcc agcgtcagcg agacgaagca gcgcagcctg atcttcgcgg cgcagcactt
     5881 cctgcgaagc tgggcctcgc caccagcctg ccggttcgac gtggtggcga tcgaggccga
     5941 ccgcatccag tggttgcaag cggccttcga cgcctcctga gggccgcctc ccggtcacgc
     6001 ctcgtgacga ttttcacgga acctgcggtc agtacggccg tcttatgatc gacgaccatg
     6061 attgaacagc gcatccagca gcagtttttc gacagcgccg atctcatgta tgcggctgca
     6121 gaagtgctca gcaagccgct cgccgaggcc gtagaagcgc tcgtggggtg catcacggcg
     6181 ggcggaaagg tactggtttg tggcaacggc ggatcggcca gcgatgccca gcatttcgct
     6241 gccgagttcg ttggccgttt cgaacgcgag cgcccggggc tggctgctct ggcgctgacg
     6301 acggacacgt ccatactcac cgcgatcggg aacgactacg acttcaactc gatctactcg
     6361 aagcaggttc aggcgctggg caatcccggc gacgtgctca tcgcgatcag caccagcggc
     6421 aattcgggca acgtgctggc tgcggtcgct gcggcgcacg ccaaggacat gaccgtcgtg
     6481 gcactgaccg gtcggtccgg cggcaagctc ggtgcgctgc tcacggacac agacgtgcac
     6541 atctgcgtgc cccacgatcg aaccgcccgc atccaggaag tccacggcct cgccctgcac
     6601 tgcctctgcg atgcggtgga tcaacagtta ctcggagaac aagaatgaat cgaatgtcga
     6661 agcttgccct ttcattggtg gctgcggcca ccgtctcgac cgccctgacg ggttgcttcc
     6721 cgctggtgct cggcggcgct gtggtcggcg gtacgtcggt ctacaccgac cgccgcactt
     6781 ccggcaccca gctcgaggac gaagccatcg agatcaaggc cctcagtcgc aactccgaag
     6841 cgatcggctc gcgcggccgg gtcagcgtga ccagctacaa caggctggcg ctgatcaccg
     6901 gcgaggtgac cagcgatgcc gaccgggcgc tgatcgaaaa gacggtctcg cgcgtcgaga
     6961 acgtgcgatc gatcgtcaac gagctcgccg tcgtgaagga actgcccacc tcgaacgccg
     7021 gtaccgacgc catcgtgacc ggcaagatca aggcatcact ggttgattcg aaggacgtct
     7081 tcgctcaggc cgtgaaggtc gtcaccgagc gcgggacggt ctacctgatg gggcgggtca
     7141 ccgaacgcga agccgctcgg gccgcagaaa tcgcacgggg cgtgagtggc gtgcagaagg
     7201 tcgtccgagt gttcgagatc atcagcgagg cggagctcgc caacctgaac gcccccgcct
     7261 cgaagtgacg cgaagcccgc ctgggcggac gatcagcgca ggcgcgtgat caggctggag
     7321 gtgtcccagc gctgcccgcc tgcggcctgg acatcggcgt agaactggtc gaccagcgcg
     7381 gtgacgggca gccgcgcgcc gttgcgcttg gcctcatcca gcaccagccc cagatccttg
     7441 cgcatccagt cgacggcaaa gccgaaatcg aattttccgt cgaccatcgt cgggccgcgg
     7501 ttgtccatct gccagctctg cgctgcgccc ttgccgatga caccgaggac ggtcttcatg
     7561 tcgagcccgg cctggtcgcc gaatgcgatg gcttcggcga gcccttgaac cagcccggcg
     7621 atggcgatct ggttgaccat cttggccagc tgacccgagc cactgtcacc cagcagcgtc
     7681 atcgccttgg caaaggccag ggccaccggc tgggcgcgcg agaaggctgc cgcatcgcca
     7741 ccgcacatca ccgtcagcga gccattgatg gcgcccaggt tcccgccgga caccggcgca
     7801 tcgatgaaac tgagcccgag tgtcgttgcc acagcacaga gttcgcgcgc cacggcagcc
     7861 gaggctgtgg tgtgatcgac gaaaaccgca cccgtctgca tgccggcaaa cgctccgccc
     7921 tcacccagca ccaccgcgcg cagatcatcg tcattgccga cgcaggcaaa gacgaaccct
     7981 gcgccttcag ccgctgcacg cggcgtcgtg gccactgtgc caccgaactc gccgacccag
     8041 gcatcggcct tggccttcgt ccggttgtag accgtgacgt gatgcccggc acgcgccagg
     8101 tggcccgcca tcgggtgacc catcacgccg aggcccagga aggccacgcg ctggggctcg
     8161 atcgactcgt aactgcgcac gccgagatct gccatgtcag acgatcgtca tgtgttcggt
     8221 gccggccgcc agatcggtgt tgcgcgcgcg ctggctgttc agcttgatct gcagtcgcag
     8281 gtcgttgacg gagtccgcgt tgcggatcgc gtcctcgtag gtcaccgcgc ggctctcgta
     8341 caggtcgaac agagcctggt cgaaggtctg catgccgtgc tcgcgggaac gcttcatgat
     8401 ctccttgatc tccgccacct cgcccttgaa gatcaggtcg gcgatcagcg gagagttcag
     8461 catgatctcc accgcagcca cgcggccctt gccttcctgg cggggcagca gtcgctggga
     8521 gaccagcccc ttcaggttca gcgacagatc catcagcaac tgcgcgcgac gctcttcggg
     8581 gaagaagttg atgattcggt ccagtgcctg gttggcgctg ttggcgtgca gcgtggccat
     8641 gcacaggtgg ccggtctcgg caaaggccac ggcgtgctcc atcgtctcgc ggtcgcggat
     8701 ctcgcccatc aggatcacgt ccggagcctg tcgcagcgtg ttcttcagcg cctgctccca
     8761 gccgtcggtg tcgatgccga cctcgcgctg ggtgacgatg cagttcttgt gcggatgcac
     8821 gaactccacc ggatcctcga tcgtgatgat gtggccgaac gagttctcgt tgcggaagtc
     8881 gaccatcgcg gccagcgacg tgctcttgcc cgaaccggtg gcgccgacga agatgaccag
     8941 gccgcgcttg gtcatcgaca cgtccttcag caccttgggc aggttcagcg aatcgatcgt
     9001 cggcagcgtc tgcggaatcg tccgcatcac catgccgatg tgaccctgct gcacgaaggc
     9061 attgacgcgg aatcgcccga tgccctgcgg cgagatcgcg aaattgcact ccttggtccg
     9121 ctcgaactca ccggcctgct tgtcgttcat cacagcccga gcgagcgcca tcgtgtgctg
     9181 cccggtcagc ggctgcggcg acaccttggt gaccttgccg tcgaccttga tggccggtgg
     9241 aaagtcggag gtcaggaaca ggtcggagcc gttgcgcgac accatcagtc gcagcaggtc
     9301 gttgacgaat ttcgaagcct ggtcgcgttc catgagattt ccttcgagtc cttagagctg
     9361 agaagagcac aagcgcagcg aagtgcctcc catcgagcaa cgcccgacgc aacggctacg
     9421 ccgggccgat gggtggcgcc cctcgggggc aggagcgcag cgactggggg gccaacatca
     9481 tccggggaaa ttctccggga acttcgcctt ggcgcgcgct tcgtgtggcg acaccacgtt
     9541 gcgccgcacc aggtcggcga ggttctgatc gagtgtctgc atgccaacgc tgttgccggt
     9601 ctggatcgcc gagtacatct gggcgatctt gctctcgcgg atcagattgc ggatggctgc
     9661 ggtgcccagc atgatctcgt gggcagcaac gcggccggtg ccatccttgg tcttgcagag
     9721 cgtctgcgaa atcacgccga tcagcgactc ggacagcatc gcgcgaacca tttccttctc
     9781 ggccgccgga aacacgtcga ccacccggtc gatggtcttg gcagcactgg atgtgtgcag
     9841 ggtgccgaac accaggtgac cggtttccgc ggcggtgagc gccagacgga tggtttccag
     9901 gtcacgcatt tcgccgacca ggatcgcgtc cgggtcttcg cgcagcgccg aacgcaatgc
     9961 gttgttgaaa ctgagcgtgt ggggcccgac ctcgcgctgg ttgatcaggc acttcttgga
    10021 ttcgtgaacg aactcgatcg gatcttcgac ggtcagcacg tgaccgtact cgttttcgtt
    10081 gaggtggttc accatcgccg ccagcgtggt cgacttgccc gaaccggtcg ggccggtcac
    10141 cagcaccatg ccgcgtggct tgagcgcgag ttcggcgaag atcttcggcg cggccagttg
    10201 ctccagcgtg aggatcttcg acggaatggt ccggaacacc gcgccagcgc cacggttgtg
    10261 gttgaacgcg ttgacccgga agcgggccag accctggatc tcgaacgaga agtcgcactc
    10321 cagaacttcc tcgtacgcct tgcgctgcga gtcgttcatg atgtcgtaga tcatcgagtg
    10381 cacatccttg tgctcgagcg gatccacgtt gattcgacgc acatcgccgt tgacacggat
    10441 catcggcggc aggcctgaag acaggtgcag gtcggaggct ttgttcttga ccgagaacgc
    10501 cagcagttgg gtgatgtcca tggggctcgc tagaaggcgg ggtcgacggg cagggtgggg
    10561 ctgcgcagca agcactgcgg taccgctgtt atcggcagat actgccaaag attatggcga
    10621 cgatcgttca gaacttgcag aaagtgcacc agcgcatcgc ccaggcgtgt gcggcggcac
    10681 aacggcctgt gcaaagcgtc acgctgctgg tggttggaaa gacattcgca gccgacacgg
    10741 tgcgcgcagc cttcgacgcc ggcgagcgct gtttcggcga gaactacgtg caggaaggca
    10801 ttgacaagat cgccgcgctg gctgacctga gggagcagat ccagtggcac ctgatcggcc
    10861 cgctccaggg caacaagacc cgcgtcgtcg cggaatcgtt cgactgggtc cactcggtcg
    10921 accggctgaa gatcgcccag cgcctgagcg aacagcgccc tgcgcacctg ccgccgctgc
    10981 agctgtgcct gcaggtcaac atcagcggcg aatccagcaa gagcgggctg accatcgacg
    11041 aattgcccga ggtggcacgc gcggtcgctg cgctgccgcg cgtgcggctg cgggggttga
    11101 tggccatacc cgcaccggcg gtcgacctgg ccgcccagcg agctccacac cgccggctgc
    11161 gggagttact ttcactgcta cagggatgcg gtctcgacgt agacaccctg tcgatgggca
    11221 tgacggcaga cctcgaggcg gccattgccg aaggcgccac catcgttcgc gtcgggacgg
    11281 cgatcttcgg cgcacgctga agcgcgcgcg tccatcggcc aaggcgaaat tgccggcgca
    11341 tcgtgacgtg ctgcacgcgc agacaggcgc cggaccgccc gtgaagggag gattccatcc
    11401 aacctgtgga gttacagtcc ttttcgactg tccgtttcaa cgatccacgc aggaaacatc
    11461 atgcaaagct cacctcgcag cggcacgacc gtcgttcgca aaaccatcac cagtctgggc
    11521 atcggcgccg ccatcctcgc cttgggagga tgcgtgtcca ccgcacctgt cgtgggcggc
    11581 ggcgaaaagg gcccggtcac cggctcggcg ggaggcgcga ccacggccaa cgcgaacagc
    11641 agcctcgagc actgcgacca gactctcggg acactcgggg tggtcgagga tcaggcggcc
    11701 tcctggttcc aggaactgcg caactacaag ctcgggtcca cggtgccggt cattcgcatg
    11761 atgatccagc agtccaactg cttcatcgtc gtcgagcgcg gcaatgccat gcagaacctc
    11821 aacaccgagc gcgcgctcca gcaaagcggc gaaatgcgcg gaggcagcaa cttcggcaag
    11881 ggacagatgg tcgcggccga ctacacgatg agcccgagca tccagttcag ccagaacaca
    11941 ggcggtgtcg ggggcgcgct gggcggcttc agcggcgcgc tgggcgtcgc cggcgcgatc
    12001 gctggcggca tgaagaccaa cgaggccgcg accacgctga tcctgatcga caaccgctcc
    12061 ggggttcagc tggcagcagc cgaaggcagc gccaagaaca tggacttcaa tgtgatgggc
    12121 ggtctgttcg gcggtggcgt cggcggaggc gccggcggct acacgaactc gccggaaggc
    12181 aagatcatca tcgcggcctt cgccgattcc tacaaccagc tcgttcgggc ggtgcgcaac
    12241 tacaaggctc agaccgtcaa gggcggtctg ggcaccggcg gcgcgctggg cgtgtcgggc
    12301 ggatcgaccg cagcctccaa ggctggcgac gccgcagccg cagcaccggc agccaagtcg
    12361 acaacgaaga cgccggcggc ggccactgcg accaagccgg ccaccaccac caagcctgcc
    12421 gccaagaagc cagccgcgaa gcctgcgacc accactacca ccaccacgcc tgcgaagacg
    12481 caatgacgat gtcgggcctt cgcgcatgac gctgtcgtcc atcgccttcg tgggcggcgg
    12541 caacatggcc ggcgcgttga tcggcgggct gctgcgcagc ggctggtcgg ctgaggctgt
    12601 cgcggtggtc gagccgagtg cggctcagcg ggagactctg gcctcccgct tcggcgtgcg
    12661 cactctcgct gaggcggacg cgacgctcgg ccgggcagac tgcgtcgtgt gggcggtcaa
    12721 gccacagctg ttcaaggcgg cctccgcgcc ctgcgcagcc catgtcggcg gcgcgttgca
    12781 gctgagcgtc atggccggca tccgcagcga cgccatcgcg ctggcgacca gcagcgaccg
    12841 cgtcgtgcgt gccatgccca acacgccggc gctgatcggc gaaggcatgt cggggcttta
    12901 cgcacgcacg gcggtgtccg cggaggagcg cacacaagtc gagaagttgc ttgcacccac
    12961 cggccagacc ctgtgggtcg accgcgagga acagctcgac gcagtgaccg cgatgtcggg
    13021 ctcgggaccg gcctacgtct tctatctgct ggaggcctgg atcgaggccg gcacggccat
    13081 gggtctgtcg gcggcgcagg cccgacagct ggcgatggcc acgctggccg gtgcgacgtc
    13141 gctggcgcgg gactccgacg agacgcccgc catgctgcgc gagcgcgtca cctccaaggg
    13201 cggcaccacc cacgccgcaa tcacgtcgct cgaagccgac ggggtccggg cggcctttgt
    13261 ccgcgcgctc aaggcggctg agcaacgggc cggcgaactg ggcgacgaat tcggtcgctg
    13321 atcgagggcg atcacagccc caccgtcgcc ggctttgccg ccatgccccg actgcctccc
    13381 gcaacacacc ccggagaccg tggaatgagc aatctgtcgt acctcaaccc gaccgccgcc
    13441 agcccctggc agacctacct gtcgcaggtc gatcgggtca ttccgcacct cgggcacctt
    13501 gcgcgctggg ccgatacgct caagcgtccg aagcgggcgc tgatcgtcga cgtgccgatc
    13561 gagatggacg acggctcggt caggcacttc gagggcttcc gcgtgcagca caacctgtcg
    13621 cgcggacccg gcaagggcgg cgtgcggtat caccccgacg tgacgctgga ggaggtcacg
    13681 gcgctcgcgg cgtggatgac gatcaagtgc gccggcgtcg ggctgccttt cggcggagcc
    13741 aagggcggca tccgcgtcga tccgaagcag ctgtcgatga aggagctcga gcgcatgacg
    13801 cggcgctaca cgagcgagat cggcatcatc atcggcccgc agcgcgacat tccggccccc
    13861 gacgtcaaca ccaacgcgca gatcatggcg tggatgatgg acacgtattc ggcgaacacg
    13921 ggcgccaccg ccaccggggt ggtgaccggc aagccgatcc acctcggcgg atcgctcggg
    13981 cgcgtcaagg ccactggccg aggcgtgttc gtcaccggcc gcgaagccgc ccggcgcatc
    14041 ggcctgaacc tcgacggagc ccgggtcgca gtgcagggca tgggcaacgt gggcagtgcg
    14101 gccgccgagc tgttcgtgca ggccggcgcc acgctggtgg ccgtgcagga ccacaccggc
    14161 acgctcgtca atccgaacgg cttcgacatg gcggagacgg ccgcggtgct caagcgcgat
    14221 cacgggatag gcaactacgc ggatgccgag cgcatcgaca acgaagactt ctgggcggtc
    14281 gactgcgaca tcctgatccc ggccgcgctc gaaggccaga tcactgcaga acgcgccgag
    14341 cgcatcaagg cgcgtctggt gctcgaaggc gcgaacggcc cgacgctccc cgaagccgac
    14401 gatgtgatga aggcgcgcgg catcctcgtc gtgccagacg tgatctgcaa cgccggcggc
    14461 gtgacggtca gctacttcga atgggtgcag gacttcagca gcttcttctg gagcgaggac
    14521 gagatcaacc tgcgcctcga caagatcatg gtcgatgccc tcaagcacat ctgggagacg
    14581 gccgatcgac accggatcag cctgcgcacc gcaactttcg ccgtggcctg cgagcgcatc
    14641 ctgaccgcgc gcgaggagcg cggcctgtac ccctgacccc tgggagtccc tgcacgctca
    14701 gactgaccgg taacggccga cctcaagtcg gccggcccgc gtccgacaat ccgtaagcat
    14761 gcgttacgtc aaacccctca gagcaaggcg cccatcgtga aaagcccctt acctcgtcgc
    14821 accgtgctgc gcagcctggc ttgcgccgct gcatggagcg cctggccggc c
//
LOCUS       DAOORV010000088        14764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_4969_length_14764_cov_6.899925, whole genome shotgun sequence.
ACCESSION   DAOORV010000088 DAOORV010000000
VERSION     DAOORV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4969_length_14764_cov_6.899925"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKV56_12125"
     CDS             <1..324
                     /locus_tag="PKV56_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOX68606.1"
                     /translation="VVAETAEESAVPVKPKGVAAVGVAAQSMALDFSPDIDLTKLNLT
                     PEASESMSGQLATLVAEQRLDQLQRVERSILRLERQNVQILGLLQKLVGSVSIVKSTS
                     DQSST"
     gene            321..1484
                     /gene="wecB"
                     /locus_tag="PKV56_12130"
     CDS             321..1484
                     /gene="wecB"
                     /locus_tag="PKV56_12130"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOX68607.1"
                     /translation="MTHRLAGEAFGPVMCIVGARPNFMKMAPILRALADHVPPIAALL
                     VHTGQHYDQDMSGQLFTDLGLPLPDINLDVGSGSHAVQTAEVMRRFEPVLDAHRPSCV
                     VVVGDVNSTLACTLVAVKKGVPVAHVEAGLRSYDRQMPEEINRVVTDQIADRLYTTER
                     SAEGNLRSEGIPAERVIFCGNVMIDSLLAHRNHARSAADTLRAHDIDPLVLEHPKGYG
                     VVTLHRPSNVDDPQTLRSLLVTLGDVAKDLPLVFAMHPRTRSNIDKLQLGALVDPARL
                     VLLPPQGYLEMLGLMSGATLVLTDSGGLQEETTALGVPCLTLRANTERPITIEQGTNT
                     LVGTDRAAILQAVAEILGGQGKRGRVPELWDGHAAERIASDLWRWLSLPAEER"
     gene            1484..2392
                     /locus_tag="PKV56_12135"
     CDS             1484..2392
                     /locus_tag="PKV56_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOX68608.1"
                     /translation="MQSPAASRGESISNALTIDVEDYFQVSAFAPYIARSDWDRRECR
                     VERNVDRILLMLDRHQTQATFFTLGWIAERYPQLIRRIADQGHEVASHGYGHQRATDL
                     NEADFLADIERAKHVLEDVCGTEVTGYRAPSFSIGVDNLWAFDCLARAGYRYSSSIYP
                     IQHDHYGMPDAPRFAHRVNDTLMEIPITTMRLLGRNLPASGGGYFRLLPYAVSRWMLR
                     QVHSREGESAIFYFHPWEIDSDQPRIRGIDRKTRFRHYVNIDRMEQRLNCLLQDFQWG
                     RMDHIFLNRAVPPAVGHSSAGHPCRT"
     gene            2425..3417
                     /locus_tag="PKV56_12140"
     CDS             2425..3417
                     /locus_tag="PKV56_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HOX68609.1"
                     /translation="MRWDRFVLSCPTATFFHRAGWQQIMAKVFRHDTHFLYSETDGQI
                     DGVLPLAHVKSMLFGNALVSLPFAVYGGVAATDEAAAMALEAEAQRIAQRLGVDHLEL
                     RHVVARHDDWPKQDLYVTFRKAILPEEEANMLAIPRKQRAMVRKGIKNGLRSDIDPGV
                     DRFFALYADNVHRHGTPALPKRYFQALRDQFGSDCEVLTVSAPDGRILSSVMSFYFRD
                     EVLPYYAGDDESARDLAANDFKYWELMRLSCARGLKVFDYGRSKQGTGSFSFKKNWGF
                     EPAPLNYEYRLYKRDAVPQNNPSNAKFRLMIETWRRMPIGLANWLGPFVVRNLG"
     gene            3423..4640
                     /locus_tag="PKV56_12145"
     CDS             3423..4640
                     /locus_tag="PKV56_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOX68610.1"
                     /translation="MANLLFLVHRLPYPPNKGDKVRSFHLLKHLAASHRVFVGTFVDD
                     PEDEAHVATLRDLCAGLHVTRLHPTRARISSLTGLMDGEALTLRYYRDAALQQWVRDT
                     VLTERIDAAVVFSSSMAQYADGTGLPMLVDFVDVDSAKWTEYAAHHRWPMSWLYQREG
                     RELLAYERSLASRAARSFFVTEKETALFRSKAPESASNCEALCNGVDADYFSPDPARA
                     SPYCAEEIPLVFTGAMDYWPNIDAVTWFASDVLPRLRDQWPSLRLHIAGRNPSPAVRA
                     LAGVAVSVTGTVPDMRPYLQHAAVVVAPLRLARGIQNKILEAMAMGRPVVAAQECAEA
                     IAARPGVELICAADADAFVREVDALLREPARAASIGLAGRLCVQRDYSWDAHLSAFER
                     HLAPLCVRQSLAA"
     gene            4659..6239
                     /gene="xrtA"
                     /locus_tag="PKV56_12150"
     CDS             4659..6239
                     /gene="xrtA"
                     /locus_tag="PKV56_12150"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HOX68611.1"
                     /translation="MSAVPADLRIVSRWRTALPAFVMLEAAILLLYRDTALVMAGIWW
                     RSETFAHCLLVVPIVLWLVWRQRDRLAELSPRPQPWLLAPMLLTALIWLLSDVTLVDV
                     GAQFALVAMLLMAVPLVLGLEVAFAILFPLLFLFFAVPFGEFVVPTMMQLTADFTVSA
                     LQISGVPVFREGQQFVIPSGNWSVIDECSGVRYLMASFMVGTLFAYLNYQSHGRRAAF
                     MLFSLVMPVVANWLRAYMIVMLAHFSGNRIATGVDHVVYGWVFFGVIIFLMFMIGSRW
                     SEPEPASSGVNASKTDLRRGSAPVVQPSSLLVWALAGVFVASLPHAALSGLLQFENSA
                     SEAKIELPSELGSGWSSKGANIVSWAPIFSNPSSEAAQAYAAAAGTVGLHVAYYRGQG
                     DDRKLVSSTNVLVLMRSDDWNLMAGGPVGLTVGTQAVSFRTAEILGRSQSATERRPHL
                     VVWRVYWIDGQFVAGDVGAKILGGLARLKGRGDEGAAIVLYADGDSVEASNATLKAFV
                     QDNLIPLNALLLQTHDAR"
     gene            6278..7456
                     /locus_tag="PKV56_12155"
     CDS             6278..7456
                     /locus_tag="PKV56_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOX68612.1"
                     /translation="MNGIDSRPLVLHLVYRFDTGGLENGVVNLINHMPASAFRHAVVA
                     LTDVVPEFASRIQREDVALISLHKAPGHGLKLYPKLQRLFRELRPAVVHTRNLAALEC
                     QVPATLAGVPVRIHGEHGRDVDDLDGTRRRYQWMRRVYRPFVNHYVALSRDLGSYLHQ
                     KVGVPDRRIAQIYNGVDIQRFHPRSGPRPDIPGCPFSGPELFMFGTVGRMQTVKAQTL
                     LAQAFVRALQLQPEQCDRMRLVMVGEGPLRTESQALLDAAGVGHLAWLPGERADVPNV
                     MRGLDCFVLPSLAEGVSNTILECMACGLPVLATDVGGNAELVLSGSTGDLVAAGNAEL
                     LAGGLLRMAAEPARALAMGGAGRSRVEQQFSLPAMVGAYQRLYEQLLAERGPHDVNRN
                     "
     gene            7462..9420
                     /locus_tag="PKV56_12160"
     CDS             7462..9420
                     /locus_tag="PKV56_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HOX68613.1"
                     /translation="MCGITGVFDTRGSRELDRVALQRMNDSQFHRGPDEGSLHIEPGV
                     GFGHRRLSIIDVATGQQPLFNEDGSVVVVFNGEIYNYQELIPELQAAGHRFHTKSDTE
                     VIVHAWEQWGEACVERFRGMFAFVLWDRNRQTLFMARDRMGVKPLYYALLDDGTLLFG
                     SELKSLLAYRGPHGSALKRDIDPLAIEEYFALGYVAEPRTVFRQARKLPPAHALAIRR
                     GEPVGEPREYWDVNFTLDNPIGQEDACAELQRQLQESVRLRMISEVPLGAFLSGGVDS
                     SAVVATMAGLSDGPVNTCSIAFDDPKFNESEFAAMVASRYATNHRVETVKSDDFDLID
                     TLARLYDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDETFGGYRRYRLHLMEERMR
                     SALPAGIRQPLFGWLGRVYPKADWAPRVFRAKTTFEGMSRTAVEAYFHSVSILRGPMR
                     ARLFSPALKRELAGYSAQEVFDFHAGRARTEDPLALIQYLDLKTYLVGDINTKVDRAS
                     MAHSLEVREPLMDHKLIEWLATLPSSMKVRGQEGKFLLKKAMEPQLPDDVLYRPKMGF
                     SVPLARWFRGPLKQRVRDAVLGERLASTGWFDRPCLQHLVEAHQSGASDYSAPLWTLL
                     MFEAFLRNVVDDASAPAIGDMHASVALA"
     gene            9417..10640
                     /locus_tag="PKV56_12165"
     CDS             9417..10640
                     /locus_tag="PKV56_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HOX68614.1"
                     /translation="MTLRILHVLDHSIPLHSGYTFRTLSILREQRLLGWETHHLTSPK
                     HTEPSAPEETVDGWHFYRTSAPSGAQLPGLAEVAMMKQTHARLLEVAQQVRPHILHAH
                     SPVLNAIPALRVGRQLGIPVVYEVRAFWEDAAVDHGTTTEGSLRYRLTRRLETHALRQ
                     AQHVFTICEGLRSDIAARGIPASKVTVIPNAVDIDAFDPGGTPDESLKAALGLQGATV
                     LGFIGSFYAYEGLDLLLDAFPQMLRRRPELRLLLVGGGPQDAALRAQAVALGVADKVV
                     FTGRVPHAEVQRYYDLVDVLAYPRHSMRLTELVTPLKPLEAMAQGRLLVASDVGGHKE
                     LIRHGETGWLFKAGSRDALADAVETLLDRRAEWPLLRQSGRRFVETQRNWASSVARYA
                     PVYASLTEAGAHAAP"
     gene            10627..11880
                     /locus_tag="PKV56_12170"
     CDS             10627..11880
                     /locus_tag="PKV56_12170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68615.1"
                     /translation="MPPPELGQPHIVVLSSLFPSSAQSGAGLFIRERMFRVGAVLPLA
                     VVAPKPWFPFQGLLRRWRPGFRPATRSHESQSGFDVWFPRFLSFPGVLKGLDGFFMAL
                     GALPRFWQLKRAGRLDLIDAHFGYPDGYAATMLGRWLNVPVTITLRGTEQRHAQDRAL
                     APRLALALQRATRLIAVSQSLRRIAIRLGVAEEKVLVVGNGVDIQKFSPVGRSEARRS
                     LGLPASAPVLVTVGGLVERKGFHRVIELLPELRKQFPGLVYLVVGGPSPEGDMGAELK
                     RQVAELGLVDAVRFMGPLAPERLREPLSAADVFVLSTRNEGWANVFLEAMACGIPVVT
                     TDVGGNSEVVCRPELGSVVPFGDQGSLRESIEHALGFDWDAAAIRRYAEDNTWDLRVD
                     ELVRLFEHLHAGGAETNGSDRVALS"
     gene            11893..13272
                     /locus_tag="PKV56_12175"
     CDS             11893..13272
                     /locus_tag="PKV56_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX68616.1"
                     /translation="MSGLYTSFTSGVLFPLHEKLKRHSTVSVRRRLEETQWWSAEQLQ
                     ALQVQRLQALLVEVGKHVPHYRDLFHALSFDPKSVNSIADLQRLPFLTKALIRARTDE
                     LKHEQATGLSRFNTGGSSGEPLIFFIGNERVSHDVAAKWRATRWWGVDIGDPEIVVWG
                     SPIELGSQDRVRAWRDKVMRTQLLPAFEMSEARLDGFIAAIRRRRPKMLFGYPSALSH
                     IALHAEKRTQRMDDLGIKVAFVTSERLYDEQRNAIQRVFGCPVANGYGGRDAGFIAHQ
                     CPSGGMHLTAEDLIVEIIDSEGRTLPPGQAGEIVVTHLATHEFPFIRYRTGDVAVMDT
                     SRCACGRGLPMLREIQGRTTDFVVAADGTVMHGLALIYVLRDLPGVRQFKIIQESRSE
                     TRVLLVTDSQFDPLRLQDIPREFRARLGPEVQISVSQVDQIPPERSGKFRYVVSHVGT
                     PTEEGSRRA"
     gene            13265..14551
                     /locus_tag="PKV56_12180"
     CDS             13265..14551
                     /locus_tag="PKV56_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HOX68617.1"
                     /translation="MRDLLVACIVFGSLPFFLKRPFWGILMLAALGYMNPHRLCYGFM
                     LSMPVVQIVAIFTLIGMLLSKEAKRMVWSREIVVLLCLIAWMGLTTTQAFYVDRAMDQ
                     YVKVLKIQILTFMTLLMLTSREKVHLFIWVIVLSLGFYGVKGGIFTILNGGAYRVQGP
                     MGTFIGGNNEMALALVMTIPLMRYLHLQEKRKFIKIGLAAGMVLSTLAAVGTQSRGAL
                     VALLITGGIFWLKSRTKLASAIFIGLSIFVVALVMPESWYERMNTIQSYEVDKSALGR
                     INAWWTAANVASDRIFGGGFDMWGPMVFQRYAPDPTNVRDVHSIYFEMLGEQGFVGLA
                     MFLGLLIMTWLKCSAVIRVARRNADLGWARDLAAMVQVSVIGYMSAGAFLGLAYFDYA
                     YHLIAVAVVVHHLVHQAGSVTDPVAVTAQGTPSTNR"
BASE COUNT         2531 a         4522 c         4643 g         3068 t
ORIGIN      
        1 gttgtggccg agactgccga ggaatcggcg gtccctgtca agccgaaggg cgtggcggca
       61 gtgggagtcg ctgcgcaatc gatggcattg gacttctcgc cagacatcga tctgaccaag
      121 ctgaatctga cgccggaggc cagcgagtcc atgtcaggac aattggccac gttggtcgcg
      181 gagcaacgac tcgaccagtt gcagcgcgtc gagagaagca ttcttcgtct ggagcggcag
      241 aacgttcaga tcctgggatt gcttcagaag ctcgtcggat cggtcagcat cgtcaagtcg
      301 acttcggacc agtccagcac atgactcatc gattggcagg cgaggccttc ggcccggtga
      361 tgtgcatcgt cggcgcgcgg cccaatttca tgaagatggc gccgatcctg cgcgcgctgg
      421 cggatcacgt cccgcctatt gccgcactcc tggtccatac cggccagcat tacgaccagg
      481 acatgagtgg gcaattgttc actgacctcg ggttgcccct tcccgacatc aacctcgatg
      541 tcggatccgg cagtcatgcc gtgcagaccg ccgaggtcat gcggcggttc gagccggtgc
      601 tcgatgcgca tcggccctcc tgcgtcgtgg tggtgggcga cgtgaattcg acgctggcct
      661 gcacactggt tgcggtcaag aagggcgtgc cggtcgcgca cgtcgaagcg ggcctgcgca
      721 gctatgaccg gcaaatgccc gaagagatca atcgggtcgt cacggatcag atcgcggatc
      781 gcctctacac gacagagcgc agcgccgaag gcaaccttcg atccgaaggc atcccggccg
      841 agcgggtgat cttttgcggc aacgtgatga tcgattcgct gctggcgcat cgaaatcatg
      901 cacgcagcgc tgcggacacc ctgcgcgcgc acgacatcga tcctctggtc cttgagcatc
      961 cgaagggcta cggcgtcgtg accctgcatc gcccatccaa tgtcgatgat cctcagacac
     1021 tgcgctcgct gctcgtcacg ctgggtgatg tcgccaagga tctgcctctg gtttttgcca
     1081 tgcatccgcg cacgcgcagc aacatcgaca agcttcagct tggcgcgctt gtcgaccctg
     1141 cccgcctggt cctgctgcct ccgcagggct atctggagat gctcgggctg atgtccggcg
     1201 ccacgctggt gctgaccgat tccggcggct tgcaggaaga aaccacggca cttggcgttc
     1261 cctgtctgac cttgcgagcc aacacggagc ggccaatcac catcgagcag ggaaccaaca
     1321 ccctggtcgg aaccgatcga gcggcgatcc tgcaggcggt cgccgagatt cttggcggtc
     1381 agggcaagcg cggccgggtt cctgaactgt gggacgggca tgcggccgag cgcattgcca
     1441 gcgatctgtg gcgctggctg agcctgccgg ccgaggagcg gtaatgcagt cgcccgccgc
     1501 aagtcggggc gaatccatca gcaatgccct gacgatcgac gtcgaagact acttccaggt
     1561 ctccgccttc gccccctaca ttgcgcgcag cgattgggat cgtcgcgaat gccgtgttga
     1621 acgcaacgtc gaccgcatcc ttctaatgct ggatcggcac cagactcagg ccacgttctt
     1681 cacgctgggc tggatcgccg aacgctatcc ccagctgata cgccggattg cggaccaagg
     1741 tcacgaagtc gccagtcacg gctatgggca ccagcgcgcc acggacctca atgaggcaga
     1801 cttcctggcc gatatcgagc gtgccaagca tgtcctcgag gatgtgtgcg gcactgaggt
     1861 gacaggctac cgggccccca gtttttccat tggcgtcgac aacctctggg cattcgattg
     1921 cctggcccgc gcgggttacc gctacagctc cagcatctat ccgatccagc acgatcacta
     1981 cggcatgccc gatgcaccgc gcttcgcaca tcgggtcaac gacacattga tggaaatccc
     2041 gatcaccacg atgcgccttc tgggccgcaa cctgcccgcc agtggcggcg gctatttccg
     2101 gctgctccca tacgccgttt cacggtggat gctgagacag gttcacagcc gcgaaggaga
     2161 gtcggcgatc ttctactttc acccttggga aatagacagt gaccagccgc gcatccgtgg
     2221 aatcgaccga aagacgcgct ttcgccacta cgtgaacatc gaccgcatgg agcagcggct
     2281 gaattgcctg ctccaggatt tccagtgggg gcgaatggac cacatcttcc tgaatcgagc
     2341 tgttccgcca gcggtcggcc actcatcggc ggggcatccg tgccgaacgt aaagtctctc
     2401 tcccttcacg atgccgcgaa tgcaatgcgg tgggatcggt tcgttctgtc ctgtccaacc
     2461 gccacgttct tccatcgtgc tggctggcag cagatcatgg cgaaggtgtt ccggcacgac
     2521 acccacttcc tctattccga aaccgatgga cagatcgacg gtgttttgcc gctggcgcac
     2581 gtcaagtcga tgctgttcgg caatgcgctg gtgagtctgc cctttgccgt ttacggaggg
     2641 gtggctgcaa ctgatgaagc tgcggcaatg gcgctggagg cggaagccca gcgcattgcg
     2701 cagcgactgg gcgtcgatca ccttgagttg cggcacgtgg ttgcccggca tgacgattgg
     2761 ccgaagcagg acctctacgt gacgttccga aaggccatcc tgcccgaaga ggaggccaac
     2821 atgctggcca ttccacgaaa gcaacgggcg atggtgcgca agggcatcaa gaatggcctt
     2881 cgcagcgata tcgatcctgg agtcgatcga ttctttgcgc tgtatgccga caacgtgcac
     2941 cgccacggca caccggcgct gccgaagcgc tacttccagg ccctgagaga tcaattcggt
     3001 tcggattgcg aagtgctcac cgtcagtgcg cccgatggcc gcattctgag cagcgtgatg
     3061 agcttctact tccgcgatga agtgctgccc tattacgccg gcgatgacga gtccgcacga
     3121 gacctcgccg cgaatgactt caagtactgg gaactgatgc gactgtcctg tgcgcggggc
     3181 ctcaaggtct tcgattacgg gcgcagcaag cagggcacgg gatccttttc cttcaagaag
     3241 aactggggct tcgagcctgc gccactgaac tacgagtacc gcctctacaa gcgcgatgct
     3301 gtgccgcaga acaatccgtc caacgccaag ttccggctga tgatcgagac gtggcgccgc
     3361 atgccgatcg gcctggccaa ctggctcggc ccgttcgttg ttcgcaatct cggctagtcg
     3421 gcatggccaa cctgcttttt ctggtgcacc gattgccgta cccgccgaac aagggcgaca
     3481 aggtccgttc gttccacctg ttgaagcatc tggctgcgag ccatcgcgtc ttcgtcggaa
     3541 cattcgtcga cgatccggag gatgaagcgc acgtcgcgac actgagagac ctgtgcgcgg
     3601 gactgcacgt cacgcgcctg cacccgacgc gcgcgcgcat ttcgagtctg acgggcctga
     3661 tggatggcga ggcgctcacc ctgcgctact accgggatgc cgctcttcag cagtgggtac
     3721 gcgataccgt gcttacagaa cgcatcgatg ccgcagtcgt tttctcgtcg tccatggctc
     3781 agtacgccga cggtacggga ttgccgatgc tggtcgattt tgtcgatgtc gattcagcca
     3841 agtggaccga gtacgctgcg catcaccgct ggccgatgtc gtggctgtac cagcgtgaag
     3901 gccgggaatt gcttgcctat gaacgcagtc tcgcttctcg cgcggcccgg tcgttcttcg
     3961 tgacagaaaa ggaaacggct ctctttcgca gcaaggcgcc tgaaagtgcc agcaactgcg
     4021 aagccctgtg caacggagtc gatgccgatt acttttcacc cgacccagcg cgcgcatcgc
     4081 cttactgtgc cgaagagatt ccactggtgt tcactggcgc catggactac tggcccaaca
     4141 tcgatgccgt cacctggttt gccagtgatg tcctgccccg acttcgggat caatggccct
     4201 cgctgagatt gcacatcgcg ggccgcaatc cgagccctgc cgttcgtgcc ctggccggcg
     4261 tcgctgtcag tgtcacgggt acggttcccg acatgcggcc gtatctccag cacgctgccg
     4321 tcgtcgtggc gccgcttcgt ctggcgcggg gcatccagaa caagattctc gaggccatgg
     4381 cgatgggccg gccggtggtg gcggcgcagg agtgtgccga agccatcgcg gcgcggcctg
     4441 gcgtcgaatt gatctgtgct gccgatgccg atgcgtttgt tcgcgaggtg gacgcgctgc
     4501 ttcgcgaacc tgcgcgagcc gccagcatcg gccttgccgg ccggctgtgc gtgcaacgtg
     4561 actacagctg ggatgcgcac ctgtcggcct tcgagcgcca ccttgcaccg ttgtgcgtcc
     4621 ggcaatcgct ggcggcctga tcgaatggaa tcatcaccat gagtgccgta cctgccgacc
     4681 ttcgcatcgt ttcccgatgg cgcacggcgc tgccagcgtt cgtgatgctc gaggctgcca
     4741 ttcttctgct ttaccgcgat accgctctgg tcatggcagg catctggtgg cgatcggaga
     4801 ctttcgcgca ctgcctgctg gtggttccga tcgtgctctg gctggtgtgg cgccagcgcg
     4861 accggctggc cgaactgtcc cccaggccgc agccttggct gctggctccg atgctgctga
     4921 ctgccctgat ctggctgctt tcggatgtga ctctggtcga tgtgggggcg cagttcgcgc
     4981 tggtggcgat gctgttgatg gccgttccgc tcgtgttggg gctcgaggtg gctttcgcga
     5041 tcttgttccc gctgctgttc cttttcttcg cggtgccctt cggcgagttc gtcgttccga
     5101 ccatgatgca actcaccgca gacttcaccg tgtctgcgct gcagatctcc ggcgtgccgg
     5161 tgttccgcga aggccagcag ttcgtcatac cgagcggcaa ctggtcggtg atagacgaat
     5221 gcagcggtgt tcgctatctg atggcgtcct tcatggtggg gacgctgttt gcgtacctca
     5281 attaccaatc gcatggccgg cgcgccgcat tcatgctctt ctcgctggtg atgcccgtgg
     5341 tggccaactg gctgcgcgcc tacatgatcg tgatgctggc tcatttctcg ggcaatcgaa
     5401 tcgccaccgg cgtggatcac gtcgtctacg gctgggtctt cttcggcgtg atcattttcc
     5461 tgatgttcat gatcggttcg cgctggtccg agcccgaacc agcatcctcg ggtgtcaatg
     5521 cgtccaagac cgacttgcgc cgcgggtcag cgcctgtcgt gcaacccagc tcgttgttgg
     5581 tctgggcgct cgcaggcgtc ttcgtggcaa gcctgcctca cgcggccctg tctggcttgc
     5641 tgcaattcga gaactctgcc tccgaggcga agatcgagtt gccctccgaa ctgggcagcg
     5701 gctggtcctc gaagggagcg aacatcgtct cttgggcgcc gatcttctcc aatccatcat
     5761 ctgaagccgc acaggcttat gcggctgcgg caggtacggt ggggctgcac gttgcttact
     5821 accgcggcca gggtgacgat cgcaagctgg tcagctcgac caatgtgctg gtgctgatgc
     5881 ggagcgacga ctggaacctg atggctggcg gacctgtcgg tctgaccgtc gggacgcagg
     5941 ccgtcagctt cagaacggct gagatcctcg gcaggtcaca gtccgccacc gagcgtcggc
     6001 cgcacctcgt cgtctggcgc gtctactgga tcgacgggca gttcgtcgct ggcgacgtag
     6061 gcgccaagat cctcggaggg ctggcgcgcc tgaagggccg cggcgacgaa ggcgcagcca
     6121 tcgtcctgta tgcggatggc gacagcgtgg aagcctcgaa cgcaacgctc aaggcatttg
     6181 tccaggacaa cctgattccc ttgaatgcac tgctgctgca gacgcatgac gctcgctgaa
     6241 tttcggcagc gctgaactgc atggggcgaa accttcgatg aacggcattg actcgcgtcc
     6301 gctggtcctg cacctcgtct atcgtttcga caccgggggc ctcgagaacg gggtcgtgaa
     6361 cctcatcaac cacatgcctg cgtcggcgtt caggcatgcg gtggtggccc tcaccgacgt
     6421 ggtccccgag tttgcgtcgc gcatccaacg tgaagacgtg gccctgattt ccctgcacaa
     6481 ggcaccgggg cacggcctga agctctaccc gaagctgcag cgtctcttca gggaactgcg
     6541 gcccgcggtg gtgcacaccc gaaacctggc ggccttggag tgtcaggtgc cggctacgct
     6601 ggcaggcgtg ccggttcgta tccatggaga gcacggccgc gatgtcgatg atctcgatgg
     6661 cacgaggcgc cggtatcagt ggatgcgtcg cgtctaccgg cccttcgtga accactacgt
     6721 ggcactttcg cgtgacctgg gcagttatct ccatcagaag gtcggtgtac cggaccggcg
     6781 catcgcgcag atctacaacg gcgtcgacat ccagcgcttt catccgcgca gcgggccgcg
     6841 tccggacatc cccggttgcc ctttctcggg tccggagctg ttcatgtttg gaaccgtcgg
     6901 tcgcatgcag accgtcaagg cgcagacgct gcttgctcag gccttcgttc gtgcgctgca
     6961 actgcagcct gagcagtgcg atcggatgcg tctggtgatg gtcggcgaag gtccgctgcg
     7021 tactgagtcg caggcgctgc tggacgcggc gggtgtgggt catctggcct ggttgcccgg
     7081 cgagcgggcg gacgtgccaa atgtcatgcg tgggctcgat tgcttcgttc tgccttcctt
     7141 ggcagagggc gtgtcgaata ccatccttga gtgcatggcc tgcggtctgc ccgttttggc
     7201 tacagatgtc ggcggcaatg ccgaattggt cctcagtggc agcactggcg atctggtggc
     7261 tgcgggcaat gccgagttgc tggccggcgg attgcttcga atggctgccg aaccggcccg
     7321 tgccctggcg atgggcggag cagggcgcag ccgcgtggag cagcagttca gtctgccggc
     7381 gatggtgggt gcatatcaac gcctctatga gcagttgctt gcagaaagag gacctcacga
     7441 cgtgaatcgg aactgaacag catgtgcggc atcaccggcg tcttcgacac tcggggttca
     7501 cgcgaactcg accgagttgc gctgcagcgc atgaacgact cccagtttca ccgcggtccc
     7561 gatgagggca gcctgcacat cgagccgggc gtgggtttcg gccaccggcg cctgtcgatc
     7621 atcgatgtgg ccacgggtca gcagccgctg ttcaatgaag acggttcggt ggtggtggtc
     7681 ttcaatggcg agatctacaa ctaccaggaa ctgatccctg aactgcaggc tgccgggcat
     7741 cgctttcaca cgaaaagcga taccgaggtc atcgtgcatg cctgggagca gtggggcgag
     7801 gcgtgtgtcg aacgctttcg tggcatgttc gcgttcgtgc tgtgggaccg caatcgccag
     7861 acgctcttca tggctcgcga tcgaatgggc gtcaagccgc tctactacgc actgctggac
     7921 gacggcacgc tgcttttcgg cagtgagctg aagtcgctgc tggcctaccg gggaccgcac
     7981 ggaagcgccc tgaaacgcga catcgatccg ctcgccatcg aggagtattt cgcgctcggg
     8041 tacgttgccg agccccgcac cgtgtttcgc caggcgcgca agctgccgcc tgcgcacgcg
     8101 ctggcgatcc gtcgcggcga gccggtgggt gagccgcgtg aatactggga cgtgaacttc
     8161 acgctggaca acccgattgg ccaggaggac gcttgcgccg aactgcagcg gcagcttcag
     8221 gaatccgtgc gcctgcgcat gatttcggag gtgccgctgg gcgcattcct gtcgggcggc
     8281 gtcgactcca gcgcggtggt ggcaacgatg gccggcttgt ctgacggccc ggtcaatacc
     8341 tgctccatcg cgttcgacga cccgaaattc aacgagagcg aattcgccgc catggtggct
     8401 tcgcgctacg ccaccaacca tcgggtcgag acggtgaagt cggatgattt cgatctcatc
     8461 gacaccctgg ctcgcctgta cgacgaacct tacgccgaca gttccgccat cccgacctac
     8521 cgcgtgtgcc agctggcgcg caagcacgtg accgtggcgc tgtctggaga tggtggcgat
     8581 gagactttcg gcggctaccg gcgctatcgc ttgcacctga tggaagaacg catgcgctcg
     8641 gcgttgcctg ccgggatccg ccagcctctg ttcggatggc tgggtcgtgt ctacccgaag
     8701 gctgactggg ctccgcgtgt gttccgcgcc aagaccacct tcgagggcat gtcgcgtacc
     8761 gctgtcgaag cctactttca ttcggtgtcg atccttcgcg gcccgatgcg cgccagactc
     8821 ttcagcccgg cattgaagcg tgaactggcc ggctacagcg cgcaggaggt tttcgacttc
     8881 catgcaggac gcgcccgtac cgaagacccg ctggcgctga tccagtacct ggacctgaag
     8941 acctatcttg tcggcgacat caataccaag gtcgaccgcg ccagcatggc ccactcgctc
     9001 gaggtgcgcg agccgttgat ggaccacaag ttgatcgagt ggctggcgac cctgccgtcg
     9061 tccatgaaag tccgtggaca ggaaggcaag ttcctgctga agaaggcgat ggagccgcag
     9121 ttgccggacg atgtcctgta tcgcccgaag atgggcttca gcgtgccgct ggcgcgctgg
     9181 ttccgcgggc cgctcaagca gcgggttcgc gacgcggtgc tcggcgaacg cctggcctcg
     9241 accggctggt tcgatcggcc gtgcctgcag cacctggtcg aggcacatca gagcggcgcg
     9301 agcgactaca gcgcgccgct gtggaccttg ctgatgttcg aggcgtttct gcgcaatgtg
     9361 gtcgatgacg cctcggctcc ggccattggt gacatgcacg catcggtggc actggcatga
     9421 cccttcgaat tcttcacgtg ctggatcatt cgattccgct acacagcggg tatacgttcc
     9481 gcaccttgtc catcctccgg gagcagaggc tgctcggttg ggaaacgcat catctgacca
     9541 gccccaagca cacggagcct tctgcccccg aggagaccgt cgacggctgg cacttttatc
     9601 gaacgtctgc gccgtccgga gcacagcttc ctgggctggc cgaggtcgcg atgatgaagc
     9661 agacgcacgc ccgcttgctg gaggtcgcgc agcaggttcg tccccatatc cttcatgcgc
     9721 actccccggt actgaacgcc atcccggcac tgcgtgtcgg ccgccagctc ggcatccccg
     9781 tcgtctacga ggttcgcgcg ttctgggagg acgcggctgt cgatcacggc accacgaccg
     9841 aaggcagtct gcgataccgc ctgacccgtc ggctcgagac gcatgcgctg cgtcaggcgc
     9901 agcatgtgtt caccatctgt gagggcctgc gcagcgatat cgccgcgcgg ggcattcctg
     9961 catcgaaggt cacggtgatt ccgaatgccg tcgatatcga cgcgttcgac cccggaggca
    10021 cgcccgatga gtccctcaag gccgctctgg gtctgcaggg tgcgacggtg ctcggtttca
    10081 tcggatcgtt ctacgcctac gaagggctcg atctgcttct ggatgcattt ccgcagatgc
    10141 tccgtcgacg ccccgagctg cgcctgctgc tggtcggcgg cgggccgcag gatgcggcgc
    10201 tgcgtgcgca ggccgtcgcg ctcggagtgg ccgacaaggt cgtgttcacc gggcgcgtgc
    10261 cgcatgccga agttcagcgc tattacgacc tggtggatgt cctcgcctac ccgcggcatt
    10321 cgatgcgcct gaccgaactc gtcacgccgc tcaagccgct ggaggccatg gcgcaaggcc
    10381 gtctgctggt tgccagcgat gtcggtggcc acaaggagct gatccgccac ggggaaacag
    10441 gctggctgtt caaggcgggc agccgggatg ccctggccga cgcggttgaa accttgctcg
    10501 accgtcgtgc tgaatggccc ctcttgcgcc aatccggccg tcgcttcgtc gagacgcagc
    10561 gcaactgggc cagcagtgtg gcgcgctatg cgcctgtata cgcgagcctg accgaggcag
    10621 gggctcatgc cgccccctga acttggccag ccgcatatcg ttgtgctgag cagcttgttt
    10681 cccagcagcg cccagtcagg tgctggcctg tttatacgcg agcgaatgtt ccgcgtgggg
    10741 gcagtcctgc cgctggcggt ggtcgccccc aagccatggt tcccgtttca agggttgctg
    10801 aggcggtggc gccccggctt ccgacccgcc acccggtcgc atgagagtca atctgggttc
    10861 gacgtctggt tcccgcgatt cttgtctttt ccaggcgtcc tgaaagggtt ggacggcttc
    10921 ttcatggccc tcggtgcatt gccgcgtttc tggcagctga agcgcgcagg ccgtctcgat
    10981 ctgatcgacg ctcattttgg atatcccgac ggctacgcgg ccacgatgct gggccgctgg
    11041 ctgaacgtgc ccgtcaccat caccctgcga ggcacggagc agcgccatgc gcaagatagg
    11101 gccctggcgc cgcgacttgc gctggctctg cagcgcgcca cccgcttgat cgcagtttcg
    11161 cagtcgctgc gacgaatcgc catccgactc ggtgttgccg aagagaaggt actcgtcgtg
    11221 ggcaatggtg tcgacattca aaaattcagt ccagtgggaa gatccgaggc tcgccgctct
    11281 ctcggtctgc ccgcaagcgc acccgtactc gtgacggtcg gcgggctggt ggagcgcaaa
    11341 ggctttcatc gtgtcatcga attgctgccc gagttgcgga agcagttccc ggggcttgtc
    11401 tatctggtgg tcggcggccc cagccccgag ggtgacatgg gcgccgaact caagcggcag
    11461 gtggccgaac tcggcttggt cgatgcagtg cgcttcatgg gacctttggc gccggagcgt
    11521 ttacgcgagc cgctctccgc tgctgacgtg ttcgtgcttt caacgcgaaa cgagggctgg
    11581 gcgaacgtct tccttgaggc catggcttgc ggaatacccg tcgtgaccac ggacgtcggt
    11641 ggcaactccg aggtggtgtg ccggcctgag ctcggctctg tggttccctt tggtgatcaa
    11701 ggctccttgc gtgaatcgat tgaacacgcg ctcggcttcg actgggatgc cgccgccatc
    11761 cgccgctatg ccgaagacaa cacctgggac ctgcgcgttg atgaactggt gagacttttc
    11821 gagcacttgc atgctggtgg cgccgaaaca aacggcagcg atcgcgttgc tctctcctga
    11881 aagccgatca tcatgtctgg cctctacacc tcattcactt caggagtgtt gttcccgctg
    11941 cacgagaagc tgaagcggca ttcgacggtc tccgtgcgcc ggcgcctcga ggaaacgcag
    12001 tggtggagcg ccgagcagtt gcaggcgctt caggtccagc gtctccaagc tctgctcgtc
    12061 gaggttggga agcatgtgcc gcactaccgg gatctcttcc acgccctgtc gttcgacccg
    12121 aagagcgtca acagcattgc cgatctgcag cgcctgccgt tccttaccaa ggcgctgata
    12181 cgcgctcgaa cagatgagtt gaagcatgaa caggcgacgg gtttgtcccg attcaacacg
    12241 ggtggcagtt caggcgaacc gctgattttc ttcatcggca atgagcgcgt cagccatgac
    12301 gtggcggcca agtggcgcgc aacgaggtgg tggggtgtcg acattggcga tcccgagata
    12361 gtagtttggg gctcgccgat cgagcttggc agtcaagacc gtgttcgcgc atggcgcgac
    12421 aaggtcatgc gaactcaact gctgcctgcg tttgagatga gtgaagccag gctcgacgga
    12481 ttcatcgctg caatccgccg gcggcgacca aagatgctgt tcgggtatcc cagcgcattg
    12541 agccacatcg cacttcacgc tgaaaagcgc actcagcgca tggatgattt gggcatcaag
    12601 gtcgcttttg tgaccagtga gcgcctgtat gacgaacaac gaaacgcaat ccagcgcgtg
    12661 ttcggttgcc cggtcgcaaa tggttacggc ggccgggatg caggattcat tgcgcatcag
    12721 tgcccttcgg gcggaatgca tctgacggct gaagatctga tcgtcgagat cattgactct
    12781 gagggacgca cgctgcctcc agggcaagct ggcgagatcg tggtcactca cctggctacc
    12841 catgaattcc ccttcattcg ctatcgcacg ggtgatgttg cggtgatgga caccagccgc
    12901 tgtgcgtgtg gccgcgggct cccgatgctt cgtgaaatcc aggggcgcac caccgacttt
    12961 gtcgtcgcgg ctgatggcac cgtgatgcac gggttggccc tgatctacgt tcttcgagac
    13021 ctgccagggg tgcgacagtt caagatcatt caggagagcc gatcggagac ccgcgtattg
    13081 ctcgtcaccg acagtcaatt cgacccattg cggcttcaag acattccccg tgagtttcga
    13141 gctcgcctgg gaccggaagt ccagatcagc gtgtcacagg tcgaccagat cccgccggag
    13201 cgctccggca aattccggta cgtggtcagt cacgtgggaa cgccaacaga agaaggcagt
    13261 cgccgtgcgt gatctgctgg tcgcctgcat tgtttttggc tcgttgccgt ttttcctgaa
    13321 acggccgttc tggggcattc tgatgctggc cgcgctgggc tacatgaatc ctcacaggtt
    13381 gtgctacggc ttcatgctgt cgatgccagt cgtgcagatc gtcgcaatct tcacgctgat
    13441 cggcatgctc ctctcgaagg aggccaagcg catggtctgg agtcgagaga tcgtggtctt
    13501 gttgtgcctc attgcatgga tggggttgac cactacgcag gcgttctacg tcgatcgcgc
    13561 catggatcag tacgtcaagg tgctgaagat acagatcctc accttcatga cccttctcat
    13621 gctgacctcg cgcgagaagg tgcatctctt catttgggtg atcgtccttt cgctgggctt
    13681 ctatggcgtc aagggaggga tcttcaccat cctcaatggc ggtgcctacc gagtgcaggg
    13741 tccgatgggg acattcatcg gtggcaacaa cgagatggcc cttgctctcg tgatgaccat
    13801 cccgctgatg cgttatctgc atcttcagga aaagcgcaaa ttcatcaaga tcgggctggc
    13861 ggccggaatg gtgctctcga ctcttgccgc cgtgggtact caatcgcgcg gagcactggt
    13921 tgccttgctg ataaccggtg gaattttctg gctgaagagt cgtacgaagc tcgcttcggc
    13981 aatcttcatc ggcttgtcga tctttgtcgt tgctctggtc atgccggaat cgtggtacga
    14041 aaggatgaac acgatccagt cctacgaagt cgacaaatcc gcactgggac gaatcaacgc
    14101 ctggtggacg gcggcaaatg ttgcaagcga ccgcatcttt ggcggtgggt tcgatatgtg
    14161 gggccccatg gtttttcagc gctacgcgcc cgatccgaca aatgttcgcg acgtgcacag
    14221 catctacttc gagatgcttg gcgagcaggg ctttgtcggc ctagccatgt tcctcgggct
    14281 tctgatcatg acctggctga aatgcagcgc cgtgattcgt gtggctcggc ggaatgccga
    14341 cctcggctgg gcccgcgatc tggcggccat ggttcaagtc agcgtgattg gctacatgtc
    14401 ggcgggtgct ttccttgggc tagcctactt cgactacgcc taccacctga tagcggtagc
    14461 tgttgtggtt catcacttgg ttcatcaggc tggatctgtc acggatcccg tggccgtaac
    14521 agctcaaggg actccgtcaa cgaaccgctg acgcgccccg tgaaatgccg ctgaactgcg
    14581 atgaagaatt tcctgccgcc gtcatccctg cgcatctgct gctcattgct ctgacctgtt
    14641 tcccgcgccg tgcatccgca gtaatgaatc cgtgacgatc gatctgaaat cagtgagtgc
    14701 gggcattgaa ggacgccgaa cagtcggacg cggtttgctg gtcgcgacac tgttgctgtt
    14761 ctgc
//
LOCUS       DAOORV010000089        14642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5027_length_14642_cov_8.556729, whole genome shotgun sequence.
ACCESSION   DAOORV010000089 DAOORV010000000
VERSION     DAOORV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5027_length_14642_cov_8.556729"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKV56_12185"
     CDS             <1..1059
                     /locus_tag="PKV56_12185"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOX68618.1"
                     /translation="LKLHEDWGTTPAAIDCCLNVAEETDTQVAIHTDTLNESGFVEDT
                     IAAFKGRTIHTFHTEGAGGGHAPDILKVVGEANVLPSSTNPTRPYTINTIDEHLDMLM
                     VCHHLDASIAEDLAFAESRIRRETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCW
                     QTAHKMKVQRGCLPGDSDRHDNTRAKRYIAKYTINPAIAHGISHEVGSIEVGKWADLV
                     LWKPAFFGVKPSLILKGGFIAAAAMGDPNASIPTPQPVHYRPMFGALGGALHQGSLTF
                     MSQSAVDGGIPDVLGLHKRIAVVKNCRTVKKQHMVHNHYAPRMEVDAQTYHVRADGVL
                     LTCEPASVLPMAQRYFLF"
     gene            complement(1086..2162)
                     /locus_tag="PKV56_12190"
     CDS             complement(1086..2162)
                     /locus_tag="PKV56_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68619.1"
                     /translation="MHNAPAAVLTLKHVRHMYGDRATVDGLSLELPAGRICCLLGPSG
                     CGKTTVLRCIGGFEPLAGGEILIESEVVSGPQRHVPPEKRRIGMVFQDYALFPHLDVE
                     RNVAFGTRGGPEDKARVDQLLDMVGLRSKARRFPHELSGGQQQRVALARALAPQPRLL
                     LMDEPFSNLDVELREQLAAEVREILKLTNTTAVFVTHDQNEAFAMADEVAVMSAGRIQ
                     QLDSAYNLYHRPTSRFVADFIGQGVFVRGKVVADRRIEIEIGSVTGSDSIRYAPDDAA
                     CGVGSQIDVLLRPDDVMHDDTSPVQGIVRHKAFRGAEFLYTLELASGARVLSMVPSHH
                     NHRLGEAIGIRLDMDHLIVFPAAT"
     gene            2302..3990
                     /locus_tag="PKV56_12195"
     CDS             2302..3990
                     /locus_tag="PKV56_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOX68620.1"
                     /translation="MTAPQSAPSSTLEMPDGRARRQRVGWLGVAALVVALFVALPIVS
                     VGTNVFSQGTGGTWAHLASTVLPDYITTTLWLCIGVGTSAAVIGVGTAWLVTHFDFPL
                     RRTFEWALVLPLAMPAYVMAYAYTDVLQFAGPVQGWLRTTMGWSRADYWFPDVRTLGG
                     AIVMFSFVLYPYVFMLARSAFMERGGAVIEAGRSLGMSPWRNFLRVSLPMARPAIAAG
                     VALALMETLADYGTVSYFAVQTFTTGIYRAWFSLGDRVAAAQLAMCLLGFVVLLLALE
                     RQSRGSARFHGSGLRRHPPRGAPLTGVRAVISVGACMLPVLIGFVLPGTVLLHMALTE
                     GDAQFGARFFALAGNSVLLSSLTALLAVALALLVAYARRLDPRALTRAAHWAVGLGYA
                     LPGSVIAVGVLIPVARMDNALAQALQSNFGWNTGLLFTGTIAALVFACVVRFMTGALQ
                     TVDSALHRVTPHMDDAARSLGLTPARALRRVHLPLLQRGLLTAGLLVFIDVMKELPAT
                     LVMRPFNFDTLATQAYTLASDERLAEASTATITIVAVGLLPLIVLCRQIARERH"
     gene            complement(3994..4980)
                     /locus_tag="PKV56_12200"
     CDS             complement(3994..4980)
                     /locus_tag="PKV56_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX68621.1"
                     /translation="MSVLFGCVQVRAQGVLNIYSARHYQTDEALYTDFTRLTGIKVNR
                     LDGKEEELVERIRNEGASSPADLLITVDAARLEIADKAGLFQPLKSAVIEARIPEALR
                     TSTWAAFSARARVVVYNKATVNAEWVQTYEDLSNPRLKNQICVRSGAHPYNLSLGAAM
                     LAHNGEARTEEWVRGLVANFARAPKGGDTDQLKAVAAGECGVAISNSYYLARLMRSTK
                     AEDQQVVRSLGVVWPNQKTWGTHINVSGAGIAKHAPNREAAVKFLEYLASDSAQAYFA
                     EGNNEWPVVKTAQVRNPALDALGPFKPDTLPIGQLAGNTALAQRVYDRAGWR"
     gene            5180..5809
                     /gene="ureE"
                     /locus_tag="PKV56_12205"
     CDS             5180..5809
                     /gene="ureE"
                     /locus_tag="PKV56_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HOX68622.1"
                     /translation="MLTVNKLISQGRGLSRVLLDRAATVSLDWDVRQKSRFDATDSQG
                     RALGVFLPRGAVVRGGDVLVAEDGSLIAVQAAPQPVIVVTHCAQHGSSFDLTRAAYHL
                     GNRHVPLELKPDHLKLEPDHVLADLLRQMHLTVTSTEAPFEPENGAYAPAAAAAHDHA
                     HGHAHESGQAHDHDHDHGHDHAPSPVRAAIPIQTGAVPHVHGPGCKHDH"
     gene            5809..6579
                     /locus_tag="PKV56_12210"
     CDS             5809..6579
                     /locus_tag="PKV56_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HOX68623.1"
                     /translation="MATRRAHQVPRAARRREHRRCASDSTRLLAPSLLQLIWLASPAL
                     PVGGFSYSEVLEAAVEAGLAATESDARNWLLDQLHLGMARGDLALVARAHSAWRRGDA
                     AEIADLNAWVMTTRESHELRQQTQQMGRSLVEWMKNRPVEPQDVELLAVLAALRPAPS
                     WPVAFALAAQQARAPARESLLCCAFSWAENMVQAALKSVPLGQSAGQRILSALLVEIP
                     DAVDAAMQLGDAERQVCTPMLAILSAQHETQYSRLFRS"
     gene            6624..7283
                     /gene="ureG"
                     /locus_tag="PKV56_12215"
     CDS             6624..7283
                     /gene="ureG"
                     /locus_tag="PKV56_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HOX68624.1"
                     /translation="MSPLHTIAHRTKKLPPLRVGVGGPVGSGKTTLVEMLCKTMRERY
                     DLVVVTNDIYTKEDQRLLTVSGALPAERIMGVETGGCPHTAIREDASINLEAVDRMLE
                     KFPDADIVFIESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDLFVIN
                     KIDLAPHVGADLEVMKSDTTRMRTSAQGLRPFVMSNLKTRQGLAEVISFIEAKGLLTA
                     G"
     gene            complement(7313..8293)
                     /locus_tag="PKV56_12220"
     CDS             complement(7313..8293)
                     /locus_tag="PKV56_12220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOX68625.1"
                     /translation="MSSDDDQSASRASASGPPGRGGWDSAAEEFARLYMQDRRSERRW
                     RVFFRLAWLVVFAVLSWTLIVDRMRATAPTGPHTALVEVRGEIDADGEASAELLVGAL
                     KNAFEDAGALAVVLRINSPGGSPVQAGIVYDEIRRLKALHNKRVYAVVEEVCASGAYY
                     IAAAADDIYTDKASVVGSIGVLMDSFGFTGTMEKYGIERRLLTAGENKGIGDPFSPLT
                     DKQRAYLQATLDQIHQQFIAVVKEGRGDRLKESPDTFSGLFWNGEEAIKLGLADHLGN
                     LDYVAREVVQAEEIIDYTPKENVAERLARRFGASIGAGAIHALHGMGRLR"
     gene            complement(8328..8711)
                     /locus_tag="PKV56_12225"
     CDS             complement(8328..8711)
                     /locus_tag="PKV56_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOX68626.1"
                     /translation="MSEPDSSAVLLCASDALVERGDAFVFDVLQWRQKATAFVMRFDG
                     RVVAYLNRCVHVPTEMDWQEGKFLDGDREFIVCSIHGATYDPNNGRCTGGPCARGQLT
                     ALRVEEHDQQVYWYPSSDTQPAPAT"
     gene            complement(8704..9366)
                     /locus_tag="PKV56_12230"
     CDS             complement(8704..9366)
                     /locus_tag="PKV56_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX68627.1"
                     /translation="MTRLRRFDLIVFDWDGTLFDSTALIARCIQASCADLDQPMPSLR
                     EASYVIGMGLTEALAHAAPGLSADRYPELSARYRHHYFARQNDLSLFEGALPMLHALR
                     QRNHSLGVATGKSRRGLDEVLHMVQLKGLFDATRTADETRSKPHPQMLNELMAELGVA
                     PDRTLMIGDTTHDLQLAANAGVASVGVSYGAHEPASFADFSPLHIAHSVADLDAWLST
                     HV"
     gene            complement(9363..10355)
                     /locus_tag="PKV56_12235"
     CDS             complement(9363..10355)
                     /locus_tag="PKV56_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX68628.1"
                     /translation="MSAPAVSRVLVDEGSEGQRLDNFLLKLLKGVPKTHVYRVIRSGE
                     VRVNKGRAAADTRLCLGDEVRVPPVRTSEHSAERSDEAPVPGREFPVVFEDEYLIAVD
                     KPAGVAVHGGSGVSFGVIEQLRQARPGAKFLELVHRLDKETSGLLLIAKKRSALLALQ
                     EQFRQRETGKTYAALVLGHWPPSKKVIDVPLHKFLSSEGERRVRTVDADDSDGRRSIT
                     LVKVVSFFDDFSLLDVTIKTGRTHQIRVHLLHEGHAIAGDDKYGDFAANRALARGDAV
                     PGARFGRMFLHARRLQFQHPATGEVIELQAPLPPECAALLHALKPRSTSAAPKP"
     gene            11065..14124
                     /locus_tag="PKV56_12240"
     CDS             11065..14124
                     /locus_tag="PKV56_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOX68629.1"
                     /translation="MKRMLINATQPEERRLAIVDGQKLLDFETEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISRNYFREGVDVRTARIQDAIREGDALLVQ
                     VEKEERGNKGAALTTMVSLAGRYLVLMPNNPRGGGVSRRIEGEDREELKENLDQLEYP
                     KGMSLIARTAGIGRTAPELQWDLNYMLKLWTAIDGAAKAGKGAFLIYQESSLVIRAIR
                     DYFTADVGEILIDTDDIYDQAQQFMAHVMPETAHKVKRYRDDAPLFSRFQIEHQIETA
                     FSRTVNLPSGGAIVIDHTEALVSVDVNSARSTRGSDIEETATRTNLEAADEIARQMRL
                     RDLGGLIVVDFIDMEESKNRREVETRLREALRPDRARVQFGSISKFGLLELSRQRLRP
                     ALSEGSHITCPRCNGTGHIRDTESSALQILRMVQEESMKENTAAVHVQVPVEVTSFLL
                     NEKRTEITKIELKQRVTVLLVPNKNLETPNYRLERLRHDDPRLENLQASYTMIEEAAE
                     ETGVTRREKTAPKQEPVIKGILPETPAPVAVPKPAPIVTPAPAPAPVQAAPAAGGGFF
                     GWVSRLFGGGTAAPSTKPAAQPIAVPATPSDGKGERGDRGARGGRGGRGSRDEKRTDR
                     GGEGRPEGRGGERRDGRGGRGGRGDRPEEATGNEAGLTAREERAPRPDRGERPERNEA
                     RSESTGEGREAGRRGSRGGRGDRAEREARVTPVAEGAPDEAQIANAFVDTQPGIVPSG
                     VESTEGGEVREGGRRRRRGGRGGRDRDESRAPAADGDAAAETGAELQPSPDEGAVVNG
                     EPAPLADAEGMAAAEGSENGERDGRRRSRGGRNRRDRGEARPEAQLGVEGSADAVADS
                     GESAATAAVPEAVAAPVAVSYTESPAPAPEAPHRPAQPPGPIAFKSLLPAADAAPAVI
                     EPVEAPEPTPAAEPTSAPAAAFVLETDALQAVAETAGLQWVNSDADKIRAAQEAMAKE
                     AKPVHVARAPKPVEAIDDGPLVLVETRKDLSQVKLPFESDAGSSATN"
     gene            complement(14212..>14642)
                     /locus_tag="PKV56_12245"
     CDS             complement(14212..>14642)
                     /locus_tag="PKV56_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX68630.1"
                     /translation="ASLMPAHVVYPKVDANPAGFSPRWLKEILRLQLGFTGAVFSDDL
                     SMAGARRIGGVEVSYTDAAEVALNAGCDLVLLCNQSVDGGAAVDALLDGLLASQTRGH
                     WHADQDSEQRRLALLPQTVPLTWDQLMHDPAYQRALERLP"
BASE COUNT         2574 a         4884 c         4762 g         2422 t
ORIGIN      
        1 ctgaagctgc acgaggactg gggcaccaca cccgccgcca tcgactgctg cctgaacgtg
       61 gccgaggaga ccgacacgca ggtcgcgatc cacaccgaca cgctcaacga gagcggattc
      121 gtcgaggaca ccatcgctgc cttcaagggc cgcacgatcc acaccttcca caccgaaggc
      181 gccggcggcg ggcatgcgcc ggacatcctg aaggtggtcg gcgaagccaa cgtgctgccg
      241 agctcgacca acccgacgcg tccgtacacg atcaacacga tcgacgagca cctcgacatg
      301 ctgatggtgt gccaccacct cgacgcgtcg atcgcggaag acctggcgtt tgctgaaagc
      361 cgaatccgcc gcgagaccat tgccgcggaa gacgtgctgc acgacctggg cgcgatcagc
      421 atgttcagca gcgacagcca ggcgatgggc cgtgtcggcg aggtggtgct gcgctgctgg
      481 cagaccgcgc acaagatgaa ggtgcagcgt ggctgcctgc ccggcgattc ggacaggcat
      541 gacaacaccc gcgccaagcg ctacatcgcg aagtacacga tcaacccggc gatcgctcac
      601 ggcatctctc acgaagtcgg cagcatcgag gtcggcaagt gggccgacct ggtgctttgg
      661 aagccggcat tcttcggcgt caagccctcg ctgatcctga agggcggctt catcgcggcc
      721 gccgcgatgg gcgacccgaa cgccagcatc ccgacaccgc agccggtgca ttaccggccg
      781 atgttcggcg ccctcggtgg agcactgcac cagggctcgc tgaccttcat gagccagtcg
      841 gccgtcgacg gaggcatccc ggatgtgctg ggactgcaca agcgcattgc cgtggtgaag
      901 aactgccgca cggtcaagaa gcagcacatg gtgcacaacc actacgcgcc gaggatggaa
      961 gtcgatgcgc agacctatca cgtgcgcgcc gacggtgtcc tgctgacctg cgagccggct
     1021 tcggtgctgc cgatggcgca gcggtacttc ctgttctgaa tcgtcacgct gcccgtgcgg
     1081 gcagctcagg tcgccgcggg aaacacgatc aggtggtcca tgtcgagtcg gatgccgatc
     1141 gcctcgccga ggcggtgatt gtggtgcgac ggcaccatcg acagcaccct cgcgccgctg
     1201 gcaagttcca gcgtgtacag gaattccgct ccacgaaatg ccttgtgacg cacgatgccc
     1261 tgcaccggac tggtgtcgtc gtgcatcacg tcatcgggac gcagcagcac gtcgatctgg
     1321 cttccgacac cgcaggcggc gtcatcgggc gcataccgga tgctgtcgct tccggtcacc
     1381 gaaccgatct cgatctcgat tcgcctgtcg gccaccacct tgccacgcac gaacacgccc
     1441 tgcccgatga aatcggcgac gaatcggctg gtcggacggt gatacaggtt gtaggcgctg
     1501 tcgagctgct ggatgcgtcc tgcggacatc actgcgactt cgtcggccat tgcaaaggcc
     1561 tcgttctgat cgtgagtcac gaacaccgcg gtggtgttcg tgagcttcag gatctcgcgc
     1621 acctcggcgg cgagttgctc gcgcagttcg acgtcgaggt tggaaaacgg ctcatccatc
     1681 agcagcaacc gcggctgcgg tgccagcgct cgggccagcg ccacgcgctg ctgctgtcca
     1741 ccggacagtt cgtgcggaaa gcgtcgggcc ttggatctca gcccgaccat gtcgagcaac
     1801 tgatcgacgc gcgccttgtc ttcagggccg ccgcgggtgc cgaaggcgac attgcgctcg
     1861 acgtcgaggt gcgggaacag cgcatagtcc tgaaacacca tgccgatgcg gcgcttctcg
     1921 ggcggcacat gccgctgggg accgctgacg acctcgcttt cgatgaggat ttcgccaccg
     1981 gccagcggct cgaaaccgcc gatgcagcgc agcacggtgg tcttgccgca gccggacggg
     2041 cccagcaggc agcagatgcg accggccggc aactcgaggc tcaacccgtc cacggtggct
     2101 cggtcgccat acatgtgtcg aacatgcttc agggtcagga cggcggcggg agcgttgtgc
     2161 atcggtgcgg atcagggaac ggcgtcgcgc tcacgaccag agccccgacc atcggggtcc
     2221 gaatgcagcg gaccgacaac cggtcgtaca ctatttgcga atcaatcgcg tttgcggcgt
     2281 gatgatgatc atatctgtcg catgaccgca ccccagtccg ccccttcctc caccctcgag
     2341 atgcccgacg gccgcgcgcg tcggcagcgc gtcggctggc tcggcgtggc agcactggtg
     2401 gtcgcgttgt tcgttgcgct gccgatcgtt tcggtgggca ccaatgtctt ttcccaaggg
     2461 accggtggca cctgggccca cctggcttcg accgtgttgc ccgactacat cacgacgacg
     2521 ctgtggctgt gcatcggtgt cggcacatcc gcggccgtga tcggcgtagg caccgcctgg
     2581 ctggtcaccc acttcgattt ccctctgcgc cgcaccttcg agtgggcgct ggtgcttccg
     2641 ctggcgatgc cggcctacgt gatggcctat gcctacaccg acgtgttgca gttcgcgggt
     2701 ccggtgcagg gatggctgcg cacgaccatg ggctggtcac gcgccgatta ctggttcccc
     2761 gacgtgcgca cgctcggcgg cgccatcgtg atgttctcgt tcgttctgta cccgtacgtc
     2821 ttcatgctgg cgcgatcggc cttcatggaa cgcggcggtg ccgtcatcga ggctggccgc
     2881 tcgctgggca tgtcgccctg gcgcaacttc ttgcgggtat cgctgccgat ggcccgcccg
     2941 gcgatcgcgg caggtgtcgc cctggcgctg atggaaacgc tggccgatta cggcacggtg
     3001 tcctacttcg cggtgcagac cttcaccacc ggcatctatc gggcctggtt ctcgctgggt
     3061 gacagggtcg ccgcggccca gctggcgatg tgcctgctcg gcttcgtggt gctgttgctt
     3121 gcgctcgagc ggcaatcgcg cggctcggcc cgctttcacg gcagcggcct gcggcggcat
     3181 ccgcctcgcg gtgctccgct gaccggagta cgcgctgtga tatcggtagg tgcctgcatg
     3241 ctgcccgtgc tgatcgggtt cgtgctgccg ggcactgtgc tgctgcacat ggcgctgacc
     3301 gaaggtgatg cgcagttcgg cgcgcgtttc tttgcgctgg caggcaacag cgtgctgctg
     3361 tccagcctga ctgcgctgct cgccgttgca ctggccctgc tcgtcgcgta tgcacgccga
     3421 ctcgatcctc gggcgctgac gcgtgccgcg cactgggcgg tcggtctggg ctatgcgctg
     3481 ccgggctcgg tgatcgcggt cggcgtgctg attccggtgg cccgcatgga caacgcactc
     3541 gcgcaggcac tccaatccaa cttcgggtgg aacaccggac tgcttttcac cggcacgatc
     3601 gctgcgctgg tttttgcctg cgtggtgcgt ttcatgactg gggcgttgca gacggtcgat
     3661 tcagcgctgc atcgcgtcac gccgcacatg gacgatgcag cgcgcagcct cggcctgaca
     3721 cctgcacgag cgctgcgtcg ggtccacttg cctctgctgc agcgcggact gctgaccgcg
     3781 ggcctgctgg tcttcatcga tgtgatgaag gaactgccgg ccacgctggt gatgcgcccg
     3841 ttcaacttcg acacgctggc cacgcaggcg tacacgctgg cgtcggacga gcggctggcc
     3901 gaggcttcaa ccgcgacgat caccatcgtg gccgtcgggc tgctgcctct gatcgtgctg
     3961 tgccggcaga tcgcgcggga acggcactag gcctcagcgc cagccggcgc gatcgtagac
     4021 gcgctgcgcc agcgcggtgt tgcctgccag ctggccgatg ggcagggtgt ccggcttgaa
     4081 cggccccagc gcatcgagtg ccgggttcct gacctgggcc gtcttgacca ccggccattc
     4141 gttgttgcct tcggcaaagt aggcttgcgc ggagtcgctg gccaggtact cgagaaactt
     4201 caccgccgcc tcgcggttcg gcgcatgctt cgcgatgccg gcgcccgaca cattgatgtg
     4261 cgtgccccag gtcttctggt tgggccaaac gacgccaagc gagcgcacga cctgctggtc
     4321 ttccgccttg gtcgagcgca tcaggcgcgc aaggtaatag ctgttcgaga tggccacgcc
     4381 gcattcacca gcggccacgg ccttcagttg atccgtgtcg ccccctttgg gtgctcgagc
     4441 gaagttcgcg accaggccgc gcacccattc ctcggtcctg gcctcgccgt tgtgggccag
     4501 catcgcggcg cccagcgaca ggttgtaggg gtgggcgccg gagcgcacgc agatctggtt
     4561 cttcagccgc gggttggaaa ggtcttcgta ggtctgcacc cattccgcat tgaccgtggc
     4621 cttgttgtag accaccacac gggcgcgcgc ggagaacgcg gcccatgtcg acgtgcgcag
     4681 cgcctccggg atgcgtgcct cgatcaccgc cgacttcaac ggctggaaca gcccggcctt
     4741 gtcggcaatc tccagccgcg cggcatcgac ggtgatcaac agatcggcag gactgctggc
     4801 gccttcattc ctgatgcgct ccaccagttc ctcttccttg ccatcgagcc ggttgacctt
     4861 gatgcctgtc agccgggtga aatcggtgta gagcgcctcg tcggtctgat agtggcgtgc
     4921 cgaatagatg ttcagcacgc cctgcgcccg aacctgcacg cacccgaaaa ggaccgacat
     4981 caggacggcg aagtgggtga accggagact tttctgcaga gcaatcatga tgagaactcg
     5041 acttgggtgg aagcgacacc aaagtgtata cgaaatcatt cgcattaaga atagtcgatc
     5101 gcccatcgaa caggcacagg actgcactcg tgccagactc gtggcccgtt gtttgctgcg
     5161 ccctgtgcgc ctctttgcca tgctgacagt caacaagctc atctcacagg gtcgcggact
     5221 cagccgcgtg ctgctcgacc gtgccgccac cgtttccctg gactgggatg tgcgacagaa
     5281 gagccgcttc gacgccaccg actcgcaggg ccgtgcgctc ggcgtgttcc tgccgcgggg
     5341 tgcggtggtg cgcggcggcg acgtgctggt cgccgaagac ggttcgctga ttgccgtgca
     5401 ggccgctcca cagccggtga tcgtggtcac gcactgtgca caacacggat cgagcttcga
     5461 cctcacgcgc gccgcctacc acctcggcaa ccggcatgtt ccgctggaat tgaagccgga
     5521 tcacctgaag cttgaaccag accatgtcct ggccgatctg ctgcgccaga tgcacttgac
     5581 ggtgacttcc accgaagctc cgttcgagcc ggaaaacgga gcctatgccc ccgctgccgc
     5641 agcggcgcat gaccacgccc acgggcacgc gcacgaatcc ggccaggccc acgatcacga
     5701 tcacgatcat ggacatgacc atgcaccgtc tcccgtgcgg gcagcgatcc cgatccagac
     5761 cggtgcagtt ccgcacgtgc atggccctgg ctgcaagcac gatcactgat ggcgacacgg
     5821 cgcgcgcatc aggttccacg ggctgctcgc cgtcgcgagc atcggcgctg cgcctccgat
     5881 tcgacccgcc tgctcgcacc ctcgctgctg caactgatct ggctggcctc tccggcactg
     5941 ccggtcggcg ggttcagcta ctccgaggtg ctcgaagcag ccgtcgaggc ggggctggct
     6001 gccaccgagt ccgacgctcg caactggctg ctcgaccaat tgcacctggg gatggcgcgt
     6061 ggtgatctgg cgctggtcgc gcgggcgcac agcgcctggc gacgtggtga tgccgccgag
     6121 atcgctgact tgaacgcctg ggtgatgacc actcgcgaaa gccacgaatt gcgccagcag
     6181 acgcagcaga tgggccgctc actggtcgag tggatgaaaa accggccggt ggagccgcaa
     6241 gacgtcgaac tcctggccgt cctggctgcg ctgcgcccgg caccgagctg gccggtggcg
     6301 ttcgcgctgg cagcacagca agcgcgggca cctgcccgcg agtcgctgct gtgctgcgcc
     6361 ttcagctggg cagagaacat ggtgcaggcg gcgctgaaat cggtgccgct cggccagtcc
     6421 gccggccagc gcatcctgag cgcactcctg gtcgagattc ctgacgccgt ggacgccgca
     6481 atgcaactcg gcgacgccga acgacaggtg tgcacgccga tgctcgcgat cctgtccgcg
     6541 cagcatgaaa cccagtattc acggctgttc cgctcctgag cggccacact tcgtcaaccc
     6601 actcgtccaa cgaacgtctc gccatgagcc cactgcacac catcgcccac cgaaccaaga
     6661 agctcccgcc gctgcgcgtc ggcgtcggcg ggccggtcgg atcaggcaag acgacgctgg
     6721 tcgagatgct gtgcaagacc atgcgcgagc gctatgacct ggtggtcgtc acgaacgaca
     6781 tctacacgaa ggaagatcag cgcctgctca ccgtgtcggg ggcgctgccg gccgagagga
     6841 tcatgggtgt ggagaccggc ggctgcccgc acaccgcgat ccgcgaggac gcgtcgatca
     6901 acctcgaggc ggtcgaccgc atgctggaga aattccccga cgccgacatc gtgttcatcg
     6961 aatccggcgg cgacaacctc gctgccacct tcagcccgga gctgagtgac ctgacgatct
     7021 acgtgatcga cgtggcggcc ggcgagaaga tcccgcgcaa gggcggaccg ggcatcacca
     7081 agagcgacct gttcgtcatc aacaagatcg atctggcgcc ccatgtcgga gcagacctcg
     7141 aggtgatgaa gtcggacacg acgcgcatgc gcacaagcgc gcaagggttg cggcccttcg
     7201 tgatgagcaa cctcaagacc cgtcagggcc tggccgaggt gatcagcttc atcgaagcga
     7261 aaggcctgct gaccgcaggc tgaagatcgg gcaacgccgc gccggccggg cgtcagcgca
     7321 accggcccat gccgtgcaaa gcgtggatgg cgcccgcgcc gatcgaggca ccgaatcgcc
     7381 tggccagtcg ttcggccaca ttttccttag gcgtgtaatc gatgatctct tcggcctgaa
     7441 ccacctcgcg ggccacgtag tcgagattac cgagatggtc agccaggccg agcttgatcg
     7501 cttcctcacc attccagaac aggccggaga aggtgtcggg tgattccttc aatcgatcgc
     7561 cccggccttc cttgaccacc gcgatgaact gctggtggat ctgatcgagc gtcgcctgaa
     7621 ggtaggcgcg ctgcttgtcg gtgagcggag agaacgggtc gccgatgccc ttgttctcgc
     7681 cggcagtcag caaccgtcgc tcgataccgt acttctccat ggttcccgtg aagccaaagc
     7741 tgtccatcag cacaccgatc gaaccgacga cgctggcctt atcggtgtag atgtcatcgg
     7801 ccgccgctgc gatgtagtag gcccccgagg cgcatacctc ctcgaccacc gcgtacaccc
     7861 gcttgttgtg cagtgccttc aggcgccgga tttcgtcata gacgattcca gcctgaaccg
     7921 gactgccacc gggtgaattg atccgcagca ccacggccag cgcaccggcg tcctcgaatg
     7981 cgttcttcaa cgcacccacc agcaattcgg cgctggcttc gccatcggca tcgatttcgc
     8041 cgcgcacctc gaccagtgcg gtgtgcggcc cggtaggcgc tgtcgcacgc atccggtcga
     8101 cgatcagtgt ccaggagagc acggcgaaaa ccaccagcca cgccaaccgg aaaaagaccc
     8161 gccagcggcg ctcgctgcgc cggtcctgca tgtacaggcg agcaaactcc tcggccgcac
     8221 tgtcccaccc gccgcgtccg gggggacccg atgcagaggc acgcgaagct gactgatcgt
     8281 cgtcggaact catggaggtc tttcgtcagc gctcgccttc gcggggctca ggtcgcgggc
     8341 gctggttggg tgtccgatga aggataccaa tacacctgtt gatcgtgctc ctccacgcga
     8401 agggccgtca gctggcccct agcacagggg cctccggtgc aacgtccgtt gttggggtcg
     8461 tacgtcgcgc catggatgga acacacgatg aattcgcgat ccccatcgag gaacttgccc
     8521 tcctgccaat ccatttcagt cggaacgtga acgcagcggt tcagataagc aaccaccctg
     8581 ccatcgaagc gcatcacgaa ggcggttgcc ttctgacgcc actgcagcac gtcgaagaca
     8641 aaggcatcac cgcgctcgac aagcgcatcg gacgcgcaca gcagcacggc ggaagaatca
     8701 ggctcagaca tgggtagaca accaggcatc gagatcggcc accgagtggg caatatgcaa
     8761 cggagaaaaa tccgcaaacg acgccggctc gtgcgcaccg tagctgacac cgacactggc
     8821 caccccggcg ttggccgcga gttgcagatc gtgggtggtg tcgccgatca tcaacgtgcg
     8881 atcgggcgcc acgcccagtt cggccatcag ttcgttcaac atctgcgggt gaggcttcga
     8941 gcgggtctcg tcggcggtgc gggtcgcgtc aaacaagccc ttcaactgaa ccatgtgcag
     9001 cacctcgtcg agacctcgcc gcgacttgcc ggtggccacg cccaggctgt ggttgcgctg
     9061 gcgcagcgcg tgcagcatgg gcaaggctcc ctcgaacaga ctgagatcgt tctggcgagc
     9121 gaagtagtgg tggcggtaac gcgcgctgag ttcgggatac cggtcggctg agagccccgg
     9181 cgccgcgtgc gccagcgcct cggtcaggcc catgccgatc acgtagctgg cctcgcgcag
     9241 actgggcatc ggttgatcga ggtcggcgca ggaagcctga atgcagcggg caatcagcgc
     9301 cgtcgagtcg aacagcgtgc cgtcccagtc gaagacgatc agatcgaagc ggcgcaatcg
     9361 ggtcatggtt tcggtgccgc agatgtcgaa cgcggcttca atgcatgcag caaagccgcg
     9421 cattctgggg gcagcggcgc ctgcaactcg atcacctcac ccgtggcagg gtgctgaaac
     9481 tgcagccgcc tcgcatgcag gaacatgcgg ccgaagcggg cgcccgggac agcgtcgccg
     9541 cgcgccagcg cccggttggc agcgaagtcg ccgtacttgt cgtcgccggc gatggcgtgg
     9601 ccttcgtgca gcagatgcac ccggatctgg tgggtccggc ccgtcttgat ggtcacgtca
     9661 agcaagctga agtcgtcgaa aaagctcacc accttcacca gcgtgatcga gcgcctgcca
     9721 tcggagtcgt cagcatccac cgtgcgcaca cgccgctcgc cttcgctcga caggaacttg
     9781 tgcaacggca cgtcgatgac cttcttcgag ggcggccagt ggcccagcac cagcgccgcg
     9841 taggtcttgc ccgtttcgcg ctggcggaac tgctcctgca gcgccagcag cgccgagcgc
     9901 ttcttggcga tcaggaggag tccggaggtt tccttgtcca gccggtggac cagttcgagg
     9961 aacttcgcgc ccgggcgcgc ctgcctcagt tgctcgatca cgccaaagct gaccccgctg
    10021 cccccgtgca ccgcgacgcc ggccggcttg tcgaccgcga tcaggtattc atcctcgaac
    10081 accaccggga actcacgccc cggcaccggg gcttcgtcac tgcgttcagc cgagtgctcg
    10141 gaggtgcgca ccggaggcac ccgcacctcg tccccgaggc acaggcgggt gtcggcggca
    10201 gcgcgcccct tgttgacgcg cacctcacct gaccgaatga cacggtagac gtgggtcttg
    10261 ggaaccccct tgagcagttt cagcaggaaa ttgtccagcc gctgtccctc ggacccctcg
    10321 tcgacgagca cccgggagac agccggcgcc gacactgacg aggctgcagg cgcggtcgca
    10381 cgcctggttt tggcccctat aatcggcagt accacgtttg cgctgtaagt gtttgatttt
    10441 tctgagttta ctcgcgcact tgcaacgtga agtggcggtc gggacaggag ttccggccac
    10501 cccgtgatgc aacgcattca gcgtgcggcg ggccccgtcc ccgagtgacc gggcagtcca
    10561 tcgttggttg cggcagagaa aacgaagaac gagcctccga agcaccgctt cggaagtaac
    10621 caaggttttc aggttgcaga ccgttgcagc aggccgcagg gccggctgcg tgcggcctgc
    10681 ggcccgcgtc cagtgaccct gcggatgtcg tctccccacc cacccacctc gcacaggctg
    10741 cacctgcccc catggccccg cttatcgggc tttggccggc ttacttgcct gacgtggccg
    10801 gaaggtgtgc gaaagaccgg ccccggatgc ccacgcgcca gcgcccttcc ggcggaccgc
    10861 gcgacggcca tcctcaggca ccgcagtgac cactgacacg caagcgccaa cgctgcggtc
    10921 ccgacctccc gggtcgtccg cagtccaggg cgattccttc tcggctctct gacgtttctc
    10981 gtgcatcgat gagtcggcgc cgccgtttgg cgtcggcatg ttgatcgagc cgtcggcccc
    11041 tgggtcgacg gcacaggagc acccatgaaa cgcatgttga tcaatgcgac gcagccggaa
    11101 gaacgccggc ttgcgatcgt cgacggccag aaactgctgg acttcgaaac cgagatcgaa
    11161 gggcgcgagc agcgcaaggg caacatctac aaggcggtcg tgacacgcgt cgagccttcg
    11221 ctcgaggcct gtttcgtcga ctacggcgaa gaccgccacg gcttcctgcc gttcaaggag
    11281 atctcgcgca actacttccg cgaaggcgtc gacgtgcgca ccgcgcgcat ccaggacgcg
    11341 atccgcgaag gcgacgcgct gctggtgcag gtcgagaagg aagagcgcgg caacaagggc
    11401 gccgcgctga cgacgatggt cagcctggcg ggccgctacc tggtgctgat gccgaacaac
    11461 ccgcgcggcg gcggtgtgtc gcgccgcatc gagggtgagg accgagaaga gctgaaggaa
    11521 aacctcgacc agctcgagta tccgaagggc atgagcctga tcgcccgcac ggcaggcatc
    11581 ggccgcaccg cgcccgaact gcagtgggac ctgaactaca tgctcaagct gtggaccgcc
    11641 atcgacggcg cagccaaggc gggcaagggt gcgttcctga tctaccagga atcgtcgctg
    11701 gtgatccgtg cgatccgcga ctacttcacc gccgatgtcg gcgagatcct gatcgacact
    11761 gacgacatct acgatcaggc tcagcagttc atggcccacg tgatgccgga gacagcgcac
    11821 aaggtcaagc gctaccgcga cgacgcgccg ctgttcagcc gcttccagat cgagcaccag
    11881 atcgagacgg cgttctcgcg caccgtcaac ctgccttcgg gcggcgcgat cgtgatcgac
    11941 cacaccgagg cgctggtgtc cgtcgacgtc aactccgcac gttcgacgcg cggcagcgac
    12001 atcgaggaaa ccgcgacccg caccaacctc gaagctgccg acgagatcgc gcgccagatg
    12061 cgcctgcgcg acctgggtgg cctgatcgtc gtcgacttca tcgacatgga agagtcgaag
    12121 aaccgacgcg aggtcgagac ccgcctgcgc gaggcgctgc gccctgatcg tgcacgggtg
    12181 cagttcggca gcatcagcaa gttcggcctg ctggagttga gccgccagcg cctgcgcccg
    12241 gcactgtccg aaggcagcca catcacctgc ccgcgctgca acggcaccgg ccacattcgc
    12301 gacaccgaat ccagcgcact gcagatcctg cgcatggttc aggaagagtc gatgaaggaa
    12361 aacaccgctg ccgtccatgt gcaggtgccg gtcgaagtga ccagcttcct gctgaatgag
    12421 aagcgcacgg aaatcaccaa gatcgaactg aagcagcgcg tcaccgtgct gctggtgccc
    12481 aacaagaacc tcgaaacgcc gaactaccgg ctcgagcgac tgcgccacga cgatccgcgc
    12541 ctggaaaacc tgcaggccag ctacacgatg atcgaggagg ccgccgagga aaccggcgtc
    12601 acccgccgcg agaagaccgc accgaagcag gagccggtca tcaagggcat ccttccagag
    12661 acgcctgccc ccgtcgccgt gccgaagccg gcaccgatcg tcactccagc gccagcgccg
    12721 gctcctgtgc aggcagctcc ggcagccggg ggtgggttct ttggctgggt gtcacgtctg
    12781 tttggtgggg gcacggcagc cccgtccacc aagcctgctg cacagccgat cgccgtgccg
    12841 gcgacgccaa gcgatggcaa gggcgagcgc ggtgaccgcg gcgcccgagg gggacgcggg
    12901 ggacgcggca gtcgcgacga gaagcgcact gatcgcggcg gtgaaggccg accggaaggt
    12961 cgaggcggtg agcgtcgtga cggccgcggc ggtcgaggcg gtcgcggtga tcgcccggag
    13021 gaagcgactg gcaacgaagc cggtctgacc gctcgtgaag agcgcgcgcc gcgcccggac
    13081 cgcggcgagc gacccgagcg gaatgaggct cgaagcgaga gcacgggtga agggcgcgaa
    13141 gccggccgcc gtggctcacg cggcggaaga ggtgatcgcg ccgagcgcga ggcccgcgtc
    13201 accccggtcg cggaaggcgc gcccgatgaa gcccagatcg cgaatgcgtt cgtcgacacg
    13261 cagcccggaa tcgtgccttc gggcgttgaa tccacggaag gtggagaggt ccgcgaggga
    13321 ggtcgccgcc gccgccgcgg tggtcgaggc ggacgtgatc gcgatgaatc acgtgcacct
    13381 gcagccgacg gagacgcggc tgccgagacg ggcgcagaac tgcagcccag ccccgacgaa
    13441 ggcgccgtcg tgaacggtga gccggcgccc ctggccgacg ccgaagggat ggctgcagcc
    13501 gaaggttccg aaaacggcga acgcgatggc cgtcggcgca gccgcggcgg acgcaatcgc
    13561 cgcgatcgcg gcgaagctcg cccggaggcg caactcgggg tcgaaggctc tgccgacgca
    13621 gtggctgatt cgggtgaatc tgccgcgact gcggcagtac ccgaggcggt cgccgcaccc
    13681 gtcgcggttt cgtacaccga gtcgccggca cctgcaccgg aggcgcccca tcgtccggcc
    13741 cagccgcctg gcccgatcgc cttcaagtcg ctgctgcctg cggcggatgc ggctcccgcg
    13801 gtgatcgagc ccgtcgaggc acctgaacca acgccggcgg cggaaccgac atcggcaccc
    13861 gccgcggctt tcgtcctcga aaccgacgcc ttgcaggccg tggccgagac cgctggtctg
    13921 caatgggtca attcggatgc cgacaagatc cgcgctgccc aggaagccat ggccaaggag
    13981 gccaagccgg tgcatgtggc tcgtgccccg aagccggtgg aggcgatcga tgacggtccg
    14041 ctggtgctgg tcgagacccg caaggacctg tctcaggtca agctgccctt cgagagcgac
    14101 gcaggttcgt cagcgacgaa ctgattcggc ccgcgaagca gcaacggcgc acctcggtgc
    14161 gccgtttttc atggtgactc ccgaagtcag gcgggcaggc cctacatcag atcagggcag
    14221 acgttccagc gcccgctggt aggccgggtc gtgcatcaac tggtcccagg tcagcggaac
    14281 ggtctgcggc agcaaggcca gcctgcgctg ttcgctgtcc tggtcggcat gccagtgacc
    14341 acgagtctgc gacgcgagca ggccgtcgag caacgcatcg acggcagcgc caccatccac
    14401 cgactggttg cacagaagca ccaggtcgca gcccgcattc agggccacct cggccgcgtc
    14461 ggtatagctc acctcgaccc caccgatgcg ccgcgcaccg gccatgctca gatcatcgct
    14521 gaacaccgcg ccggtgaagc cgagttgcag gcgcaggatt tccttgagcc agcgcggcga
    14581 gaaccctgcg gggttcgcat cgaccttcgg atagacgaca tgggccggca tcaggctggc
    14641 ga
//
LOCUS       DAOORV010000090        14366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5162_length_14366_cov_13.945147, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000090 DAOORV010000000
VERSION     DAOORV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5162_length_14366_cov_13.945147"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKV56_12250"
     CDS             complement(<1..1003)
                     /locus_tag="PKV56_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX68631.1"
                     /translation="MEFDQLHPAMQYHVVNSLGWSTLRPTQLAAIAPIHAGTHCLLLA
                     PTAGGKTEAAAIPMLSRMLREEWPAASVLYICPIKALLNNLEQRLSYYAGLVGRRVEV
                     WHGDVSQSRKQRALKDAPDILLTTPESVEAMLISTRVDQQAWFGNLQAVIVDELHAFA
                     ADDRGWHLRSVLHRLDQYLKRPLQRVGLSATVSNPQELLDWLAPSGKREVVGSASVST
                     DAEVTIDHVASLENAATVIARLHRGQKRLVFCDSRSSAEQLSSMLRAHEVRTFLSHAS
                     LSASERRQAETAFAEEKDCVIVATSTLELGIDVGDLDRVIQIDAPSTVSSFLQRMGRT
                     "
     gene            complement(1003..2313)
                     /gene="brxD"
                     /locus_tag="PKV56_12255"
     CDS             complement(1003..2313)
                     /gene="brxD"
                     /locus_tag="PKV56_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HOX68632.1"
                     /translation="MISEARREDIVGALRRGTVPSSGLDALAVGIESFAPTLDEELAA
                     VAAGRGGFKAVRGEYGTGKTFFGRWLQERARARGFAASEVQINETETPLHKLETVYRR
                     LVERLGTADTGVGAFRGVIDGWFYALEQDVLADESVDASNADDLLARTDALMEARLAT
                     INKAAPAFSAVLRTYRRAVADGDAMLADGLISWLAGQPNVAASVKRAAGIKGDLDHFG
                     ATNFIVGLLTILRDSGFAGLVLVLDEVETLQRMRTDTREKGLNALRQWIDEIDAGRYP
                     GLYLVVTGTPAFFDGPQGVQRLAPLAQRLHVDFGTDRRFDSPRAVQIRLSPFDHGALL
                     SVGRRVRDIYADGRPHEMRLRAVVDDAYVDTLATAVAGGLGGKVGIAPRLFLKKLVAD
                     VLDRVDLHADFDPRKHYSLTLAESEMSVAERAATGASTVDDIEL"
     gene            complement(2310..5036)
                     /gene="pglZ"
                     /locus_tag="PKV56_12260"
     CDS             complement(2310..5036)
                     /gene="pglZ"
                     /locus_tag="PKV56_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HOX68633.1"
                     /translation="MSLSTPQIAAQLDRVLSSDPTAAAVAIRATARQPWPESLTQRGR
                     QFSLRWCESSLAIREALCDNEQHDPASAGLVVITPLATHEVAEDIAARLARGRVYQPE
                     GWDIVRQLFQAKETDARLGRFAWMPQALIDGAAQGQYTPVANGFLDLETAWREVLQRF
                     LGLKSARPDAVALLQWCSLPGSDAALRLMPAATRTDLTRWLSEAAGTAGAMVLACVDA
                     GRTADALALGLVCGVVYAPAGEGQAALGQAAIRLERYVGDKHVGIAEGRAWSQAAEQA
                     VRTAGPDAMRGALDRADSLLNELRIAEFAHLSDLLPSSLDQRMRAFAAALSAHVAAPG
                     EAQLARVEQLADGVLKHALAAGQQQRMERVEMARRLARWLMLPAPSAANVTTAVGWQA
                     DEGAFVDWARFRLLGGDELTELDHAYDALRAAAIARRNAFDQPFAQTLLQWNAQTPGA
                     DGRVVPIESVLDRVLGPIAASQPVLLLVMDGLSTSIYRELFSRINNLGWVELVPADVE
                     HPLVGVAALPTVTEVSRTSLLCGRLALGTAANEKTGFANHTALLAHSRSEVPPRLFHK
                     GELADSTNLSQEVRATIANPQQRVVGVVYNAVDDHLSGPDQLHQRWALEDLRLLLPLL
                     REAREARRVVVITADHGHLLEDGTTQVTGASAPDKVSDRWRPGRDARAPHEVAFSGGR
                     VVTADNSKAVVCLWGESTRYAGRKNGYHGGVSPQEVAVPLSVVVPLGMNLAGWQLAPP
                     AQPEWWELPHLAPVPAPAAAPAPAARPAGRKTPTAPVAQPSLFEPQEAPTLPPIAAAA
                     AADDWIGALLSSSIYASQRQLAARVALPDDKMRLLLVSLSERGGKLSRAALAQRLALP
                     EVRLGGLLSAVRRMLNVDQAPVLTVDESAGTIELNRALLMQQFRVSTQGGPR"
     gene            complement(5033..>6819)
                     /locus_tag="PKV56_12265"
     CDS             complement(5033..>6819)
                     /locus_tag="PKV56_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HOX68634.1"
                     /translation="TWRGTKREVELLYENVREMTDDRLRGRSGAWTVVLDFPFDDANF
                     GPADDLARLGEYRGGDTRTLVWLPSFLSNKALADLGRLVVLDYILQGDRFDNYAGHLS
                     FVDRVQAKALAKNQLDQLRIKLRSQLEVAYGISTEPRDAVSNALSADQQFKSLDPTLS
                     PRPPVGADFRAAFESLLGQLFAHQYPAHPEFETEIKASVIKKVWPEVQKAIEATGQRG
                     LVQDASTRKLVRAVVNPSQLGQMGETHLLIEPHWQARFAQSHARDGGGVITVAKLRQW
                     IDTPAPMGLPLELQNLIILTFAATTNRRFVMRGGPYEPSIDSLPDELELREQALPDAG
                     HWQTAVQRASSLFGLTPAQVLNAANVGRLVDDVRQAATQKRDAAARLVAQVRDRANRY
                     APGVTGARQQTAESAQALLASLTQAAEGDMVAALASATLQTSDAAVGRTLGQAQVCAD
                     TLTNTRWDMFDAMRDLQDHRRDAAASVLSRLSEVLTSDEHVVPLKSRLEELERDAMRL
                     LTAAAPPPPAPAPTPAGIPTAATPAPVALPPAPAAMGPEVVEEKQQLHLSGAAAAAAL
                     DDLKARMAKDQDLELTLSWRLQRKGTQL"
     assembly_gap    6820..6919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6920..8535)
                     /locus_tag="PKV56_12270"
     CDS             complement(<6920..8535)
                     /locus_tag="PKV56_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HOX68635.1"
                     /translation="MTLLRDLIAIPERVHQGDFVLKLSEGVTHADQTLRDYVVTPQLV
                     DAFSNALGFIQQAVQTGGSKAAYLHGSFGSGKSHFMAVLNLLLAGNTQARATPELADV
                     VARNGWTTGRKFLLVPYHMVGARDMESAVLGQYAEFVRKKHPEAPVPGFYLAEGLFKD
                     ASALRLRMGDESFFAKLNEGAKHEGGGSGGASGWGSFDSGWDAASFEAAMLEAPNGEE
                     RSRLVGDLISQFFSAYRTIAGSGESYVSLDDGLSIMSRHARDLGYDAVILFLDELVLW
                     LASHAADVNFVSREGTKLVKLVEATNADRPIPLVSFVARQRDLRDLVGENLAGVVQVQ
                     FSDVLKHWEARFHRITLEDRNLPAIAEKRVLKPVSEAARQTMQVAFDNILNMRRDVLD
                     TLLTTTADRDMFRKVYPFSPALVQTLIAVSAALQRERTALKLMLQLLVDRRADLELGQ
                     LIPVGDLYDAIAEGDEPFSEGMRLHFENAKRLYTQRMLPMLERQHGVTWDAIKLGQAD
                     ATAAKNLRNDARLLKTLLLGALVPEVESLKG"
     gene            complement(8588..9331)
                     /locus_tag="PKV56_12275"
     CDS             complement(8588..9331)
                     /locus_tag="PKV56_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68636.1"
                     /translation="MKLVLSGEGPTDLGHERPSATGMEFVPGPMAWVVDKLLERHHTA
                     YSMLEAHLAGADCATYVHETRLAAMGKPSSPLFPGVKFGKGMAFYTRNAQALGLFANQ
                     TALEGDQPVMAVLFRDGDGTRAMPANDWQRKVESMHRGFELVGCESGVPMVPRPKSEA
                     WLLCALRPPGYANCAPLEDASGNDASPNSLKRQLEALCGGNAPSADEQADWVATGAVD
                     PLRIDMPSFNRFKEALHAAANRAGLPTLP"
     gene            complement(9328..10716)
                     /locus_tag="PKV56_12280"
     CDS             complement(9328..10716)
                     /locus_tag="PKV56_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68637.1"
                     /translation="MSRFRCGLSEAKKRALESQRQWRRQCTVAANPRWQHERPNQELD
                     AQVRSRIQTMIRSFYIDNFKSLVDFRLPPAKQQLGPFTCLVGLNGAGKSTVLQAFDFI
                     GHLANGQIGAWLKQREWKRADLTSQFLKKKVLIKFELGVDVPSIGHIVWSGTYNTTLM
                     RCTAETVTLDGVEQVLHISDGQLLVSGTDTKDSVQYPLRSMKFEGSTLSFVNVRDVHE
                     ALGALKHVAANLRSLDLLSPQSMRKRAKDAADIGYGGERLSAFIHGLPPADRTQLAKA
                     LKHFYPQVAELATKALRAGWKDLRIVEQYDSANLFPTRLETDARQVNDGLLRVLAVLS
                     QVSVSEPEDSTHQPTGLGQSCVLFDEIENGINPELMQKLVELLLHAKRQVIVTTHSPL
                     VLNYLPDDVAKESVLLLFRNTAGHTKVIRLFDLPSTQKKLKLLGPGEVFVDTHLEALP
                     AEAMALLDTQAP"
     gene            complement(10607..11317)
                     /locus_tag="PKV56_12285"
     CDS             complement(10607..11317)
                     /locus_tag="PKV56_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOX68638.1"
                     /translation="MDAQHVKNRLCFLTCSGETFVWPTRALRADRPVGIRSRQARTYH
                     DDKFSPVASGRFARPTIVSDTTAESVCPACKAGQLHTVTRTRDFRPRGRLVTVELLGS
                     RCDACGVETTRAPQHDENLRRLAARKAQYGNTLLGEEIVGLRKRYGLTQVAAARVFGK
                     GKIAFSRYENEATYPDASTTLLLTMAIQMPDSLKWLADKAGVEIPLWPERSEEARAGE
                     PKAVAAPVHRSRKSKVAT"
     gene            complement(11332..11544)
                     /locus_tag="PKV56_12290"
     CDS             complement(11332..11544)
                     /locus_tag="PKV56_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68639.1"
                     /translation="MPRAAEVEAAAVLKRAIAKSHAPAELKGMGTRQTCSKYLRELER
                     LDVPHGQKIGREVYFINQAPFKLLTR"
     gene            complement(11587..12885)
                     /locus_tag="PKV56_12295"
     CDS             complement(11587..12885)
                     /locus_tag="PKV56_12295"
                     /inference="COORDINATES: protein motif:HMM:NF040337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6161 domain-containing protein"
                     /protein_id="HOX68640.1"
                     /translation="MEACSGGIKARPEEGEQGGLMNTNTTPEPRPEPLFSLDLKDNGG
                     LFAPTSLAEVNSWTQHEVNFWSWLNNSRAGGNHDQGLKQALNQLNQAQNTANQAQQYK
                     DSNPQHYQNQISQLRTHLEEAFLRQHLPHSSTPQAKRIEDYRKEAGDHPASFFASVFV
                     PPQQGHHFQPQELVGWRGLIEGLVERYRLTSAPAKGRKQAADQSFEQLRVKAEALLGE
                     KTTAYDALHRDYSALADSIKTAADQQKTDFDAAQAKRDGDFEKLVTEHKEEMEKLRKT
                     FREEIALRAPATYWDDKRKEHDRLSKITGAISFAGIAGAAGGLGWLIHDLLRSATPGA
                     APEAWRVAMLVLIGLFAVWGVRLVVRMFLSHLHLAGDAAERVVMARTYLSLLEGDRLA
                     SKEDRQLILQALFRPSSDGIVKDEGIPPSALEFLTRQSKG"
     gene            complement(12825..14258)
                     /locus_tag="PKV56_12300"
     CDS             complement(12825..14258)
                     /locus_tag="PKV56_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68641.1"
                     /translation="MIAGKADTIAKTREALMPSAIAARAEVLNRAVLDSSHLEAERLL
                     GELIGIQEELDWHCYRIYDLLPATGKGAELECATPPELALGERAFEIVMARRVSAGIE
                     TTNWFERHGSRPITELPKHWPPDYRAVVERRIALIESDRNIGLIERPEYKRRWNTPAW
                     QDLEQDAMRYWLLDRLEKPALWPCSAEVPPQLTTTNRLAELARADAEFMQVAALYAGH
                     TDFEPARLVAELVAAEAMPLLPVQRYTNDGLRKRAQWEDTWVLQRREDAIDAEIDQLE
                     EARRLELIALVTKAAECATPDADALAWVRQELDKTFIPLRKDRKAREVGPIPAPPKYK
                     SSDFLRADLWHLRGGLDVPKERFVSFPGAERGADGSLPIAWAGWNHLQLATALAGYYG
                     EMKNNEGWEPARLQPLLAGLLELVPWLEQWHNDLDPEFGQRMGTYYRSFVTEDARELG
                     FTLDDLRAWKPAVVASKRGRKKANREA"
BASE COUNT         2372 a         4749 c         4490 g         2655 t
ORIGIN      
        1 cggttcggcc catgcgctgc aggaacgatg acaccgttga cggcgcgtcg atctgaatga
       61 ccctgtccaa gtcgcccaca tcgatgccga gttccagcgt cgaggtcgcg acgatgacgc
      121 agtccttctc ttcggcaaag gcggtttccg cctgtcggcg ttccgacgca cttagcgacg
      181 cgtggctcag gaaggtgcgc acttcgtgcg cccggagcat gctgctgagt tgctcggcgc
      241 tgcttcgtga gtcgcagaag accaagcgct tttgcccacg gtgcagtcgc gcaatcacgg
      301 tcgccgcgtt ttccagcgag gcgacatgat cgatcgtcac ctcggcatca gtgctgacgc
      361 tggcactgcc gacgacttcg cgtttgcctg aaggtgcaag ccagtccagc agctcttgcg
      421 ggttgctcac ggtggccgac aggcccacac gctgcagcgg tcgcttcagg tactgatcga
      481 gccgatgcag caccgagcgc agatgccagc ctcggtcgtc ggctgcgaag gcatgcagct
      541 cgtccacgat cacggcttgc aggttgccga accaggcctg ttggtcgacc cgggtcgaga
      601 tcaacatggc ttcgaccgat tccggtgtcg tcagaaggat gtctggggca tccttcagag
      661 cacgctgttt tcgtgattgc gaaacgtcgc cgtgccagac ctcgacgcgc cgaccgacca
      721 atccggcgta gtaggaaagg cgctgttcca ggttgttcag cagcgccttg atcgggcaga
      781 tgtagagcac gctggctgcc ggccactcct cgcgcaacat gcgcgaaagc atcggtatcg
      841 ctgctgcctc ggtcttgcca ccggccgttg gcgcgaggag caggcagtgc gtgccagcgt
      901 ggatcggcgc gatggcggcc aactgggttg gccgcaaggt gctccagccc agcgagttga
      961 cgacgtggta ctgcatcgca ggatgcagtt ggtcgaactc catcacagct cgatgtcgtc
     1021 caccgtcgat gcgcctgtgg ccgcgcgctc tgcaaccgac atttcgctct cggccagtgt
     1081 caggctgtag tgctttcttg gatcgaaatc ggcatgcagg tccacgcggt ccagcacatc
     1141 ggctacgagc tttttcagga agaggcgagg cgcaatgccg accttgccgc ccaggccgcc
     1201 cgctaccgct gtggccaggg tatcgacgta cgcgtcgtcc accacagcgc gaaggcgcat
     1261 ctcatgtggc cgcccgtcgg cgtagatgtc gcgcacccgg cgcccaaccg atagcaacgc
     1321 accgtggtca aacggcgaca gccgaatctg cacagctcgc ggactatcaa aacgcctgtc
     1381 ggtgccgaag tccacgtgca gccgctgtgc cagcggtgcc agacgttgca cgccctgcgg
     1441 gccgtcgaag aacgcaggag tgccggtcac gacaagatag agccccgggt aacgcccagc
     1501 gtcgatttca tcgatccatt ggcgcagcgc gttcaggccc ttctcgcgcg tgtcggtgcg
     1561 catgcgttga agggtttcca cctcgtccaa caccagcacc aggcctgcga acccgctgtc
     1621 gcgcaggatg gtcagcaggc cgacgatgaa gttcgtggcg ccgaagtggt ccaggtcgcc
     1681 cttgatgccc gctgcgcgtt tcacgctggc ggccacgttg ggctggccag ccagccaact
     1741 gatcaggcca tcggccagca ttgcgtcgcc gtcggcgacg gcacggcgat aggtgcgcag
     1801 caccgccgag aaggccggcg ccgctttgtt gatggtggcc agccgggcct ccatcagggc
     1861 gtccgtccgg gccagcagat catccgcgtt gcttgcgtcc accgactcat cggccagcac
     1921 gtcctgctcc agcgcgtaga accatccgtc gatcacgccg cgaaaggcgc cgacgcctgt
     1981 gtctgccgtg cccagccgct caaccagtcg gcgatacacc gtctccagct tgtgcagcgg
     2041 cgtctcggtc tcgttgatct gaacctcgct ggcggcaaag cctcgcgcac gggcgcgctc
     2101 ctgcagccag cggccgaaga aggtcttgcc ggtgccgtac tcgccacgaa cggccttgaa
     2161 tccgccacgg ccggctgcca cggcggccag ctcctcgtcc agggtcggtg cgaaagactc
     2221 gatgccaacg gccagtgcgt ccaagccgct gctgggcacg gtgccacggc gcagggcgcc
     2281 gacgatgtct tcgcgccggg cttcgctgat catcgaggcc ctccttgcgt gctgacccgg
     2341 aactgctgca tcaacagcgc ccggttcagt tcaatcgtgc ccgccgattc gtcgacggtc
     2401 agcaccggag cctggtccac attgagcatg cgccgcacgg cgctcagcaa gccgccaagc
     2461 cgcacttcgg gcagcgcgag tcgctgtgcc agggcagcac gcgacagctt gccgccgcgc
     2521 tcggacagcg acaccagcag caaacgcatc ttgtcgtcag gcagcgctac gcgcgctgcc
     2581 aactggcgct gcgacgcgta gatcgagctg gagagcaacg ccccgatcca gtcgtcggca
     2641 gcggcggctg ccgcgatcgg gggcagggtg ggtgcctctt gcggctcgaa caacgagggt
     2701 tgcgccaccg gcgcagtagg cgtctttcgg cccgccgggc gtgccgcagg tgccggcgcc
     2761 gcagccggtg ccggcacagg ggcaaggtgc ggcaattccc accactcggg ttgcgccggt
     2821 ggggccagct gccagcccgc gaggttcatc cccagcggca cgactacgct cagaggcacc
     2881 gccacctcct gtggcgagac gccgccgtga tagccgttct tcctgcccgc gtaccgcgtg
     2941 ctttcacccc acaggcagac cacggccttc gagttgtcgg ctgtcacaac acggccaccg
     3001 ctgaaagcca cttcgtgcgg ggcgcgtgca tcgcggcccg gacgccaccg gtcactgacc
     3061 ttgtccggcg cggatgcgcc ggtcacctgg gtcgtgccgt cctccagcag gtgcccgtgg
     3121 tcggcggtga tgacgaccac ccgtcgcgct tcgcgcgcct cgcgcaacag cggcagcaac
     3181 aggcgcaggt cttccagcgc ccagcgctga tgcaactggt caggcccact caggtggtca
     3241 tccacggcgt tgtagaccac gcccaccacc cgctgttgtg ggttggcgat cgttgcgcgg
     3301 acctcctgcg acaggttggt cgaatcggcc agttcgcctt tgtggaatag gcgcggtggc
     3361 acctcgctgc ggctgtgtgc cagcagcgcg gtgtggttcg cgaacccggt cttctcattg
     3421 gcagcggttc caagggcgag ccggccgcag agaaggctgg tgcggctcac ttcggtgacc
     3481 gtgggcagcg cggcgacgcc gaccagcgga tgctcgacat cggccggcac cagctcgacc
     3541 cagccgaggt tgttgatgcg cgagaagagt tcgcggtaga tgctggtgct caagccgtcc
     3601 atcaccagca gcagcaccgg ctggctggcg gcgatcgggc cgagcactcg gtccagcacc
     3661 gattcaatcg gcactacgcg tccgtcggcg ccaggcgtct gagcgttcca ctgcagcagc
     3721 gtctgggcga atggctggtc aaaggcattg cgacgggcga tcgcggctgc gcgcagcgcg
     3781 tcgtacgcat gatcgagttc ggtcaactcg tcgccgccca gcagccggaa gcgtgcccag
     3841 tcgacaaatg cgccttcgtc cgcctgccaa cccacagcgg tggtcacgtt ggcggccgat
     3901 ggtgccggca gcatcagcca ccgtgccagg cgcctggcca tctcgacacg ctccatgcgc
     3961 tgctgctgcc ctgcggccaa ggcgtgcttc agcacgccgt ctgccaactg ctccacccgt
     4021 gcaagctgtg cttcgcctgg cgcggcaacg tgtgccgaca aggccgcagc aaacgcccgc
     4081 atccgttggt ccagggacga gggcagcagg tcgctcaagt gggcgaactc cgcgatgcgc
     4141 agttcgttga gcagactatc agctcgatcc agggcgccgc gcatggcgtc tggcccggct
     4201 gtacggacag cctgctcggc cgcctgcgac caagcacggc cttcggcgat gccgacatgt
     4261 ttgtcaccca cgtagcgctc aaggcgaatg gccgcttggc ccagcgcggc ctggccttcg
     4321 ccagccggcg catagaccac gccgcacacc agccccagtg ccagcgcatc ggcggtgcgg
     4381 ccggcatcca cgcaggccag gaccatggcg ccggcggtgc cggcggcctc gctcagccag
     4441 cgtgtgagat cggtccgcgt tgcggccggc atcagcctga gcgcagcatc ggagcccggc
     4501 agcgagcacc actgaagcag tgccacggca tcgggccgcg cggacttcag cccgagaaag
     4561 cgttgaagca cctcgcgcca agccgtctcc agatcgagga agccgttggc caccggtgtg
     4621 tactggcctt gagccgcgcc gtcgatcaag gcctgcggca tccatgcaaa gcgtccgagc
     4681 cgcgcatcgg tttccttggc ttggaacagc tggcggacga tgtcccaccc ttcgggttgg
     4741 tacacgcgcc cgcgcgccag cctggcggcg atgtcttcgg ccacctcgtg cgtggccagt
     4801 ggcgtgatca ccaccagtcc ggcagaggca gggtcatgct gctcgttgtc gcacagcgcc
     4861 tctcgtatcg ccaacgacga ctcacaccac cgcagcgaaa actgccgccc gcgctgggtc
     4921 agcgactcgg gccacggttg ccgcgcggtc gcacgaatgg ccaccgccgc cgcagtcggg
     4981 tcgctgctca gcacccgatc caactgcgct gcgatctgtg gcgtcgataa actcatagct
     5041 gcgttccctt gcgctgcagt cgccagctca gcgtcaactc aaggtcctga tccttcgcca
     5101 tccgagcctt caggtcgtcc agtgctgcgg cggccgctgc gccgctcaga tggagctgct
     5161 gcttctcttc caccacttcc gggcccatcg ccgccggtgc aggcggcaag gccaccggcg
     5221 caggcgttgc cgccgtcggg atgccagccg gcgtcggggc aggcgcgggg ggtggtggtg
     5281 cagctgccgt cagcagccgc atcgcgtcac gctccagttc ctccagccgc gacttcagcg
     5341 gcacaacatg ctcatcgctc gtcagcacct cgctcaggcg gctcagcacg gacgcggcgg
     5401 cgtcgcgtcg gtgatcctgc agatcgcgca tggcgtcgaa catgtcccag cgggtgttcg
     5461 tcagcgtgtc ggcgcagacc tgcgcctggc ccagcgtgcg gcccaccgcc gcgtcagaag
     5521 tctgcaacgt ggccgaggcc agcgctgcca ccatgtcgcc ttcagccgct tgggtcaaag
     5581 acgccagcag cgcctgtgcc gactctgccg tctgctggcg cgcgccggtc acgccagggg
     5641 catagcggtt cgcccgatcg cgcacctgcg ccacaagccg agcggcggca tcgcgcttct
     5701 gcgtggccgc ttgccgcacg tcgtccacca ggcggccgac gttggccgca ttcagcacct
     5761 gtgccggggt caagccaaac aggctggacg cgcgttgcac tgcggtttgc caatggccag
     5821 cgtcgggcaa ggcctgctcg cgcagttcca gttcatcggg caggctgtcg atgctgggct
     5881 cgtacggccc accacgcatg acgaaccggc ggttcgtcgt ggccgcaaag gtcaggatga
     5941 tcaagttctg cagttccagc ggcagcccca tcggtgcggg tgtgtctatc cactggcgca
     6001 acttagccac agtgatgacg ccaccgccat cgcgtgcgtg gctctgcgca aatctcgcct
     6061 gccagtgcgg ctcgatcagc aggtgcgtct cgcccatctg ccccagctgg ctggggttca
     6121 ccacggcacg caccagcttg cgggtagagg cgtcctgcac caggccgcgc tgcccagttg
     6181 cttcgatcgc tttctgcacc tcaggccata ccttcttgat gacgctggcc ttgatttcgg
     6241 tttcgaactc cgggtgtgcg gggtactggt gcgcaaagag ttggcccagc aagctctcaa
     6301 acgcggcgcg gaagtcggcg ccgaccggcg gcctcggcga cagcgtcggg tccagtgact
     6361 tgaattgctg gtcggccgac aacgcgttgc tcaccgcatc acgtggttcg gtgctgatgc
     6421 cgtatgccac ttccagctgc gaccgaagct tgatgcgcaa ttggtccagc tggttcttgg
     6481 ccagcgcctt ggcctgcacc ctgtcgacaa acgacagatg gcccgcgtag ttgtcgaagc
     6541 ggtcgccttg caggatgtag tccagcacca caaggcgtcc cagatcggcc agcgccttgt
     6601 tgctcaggaa cgagggcagc cacaccaacg tgcgcgtgtc accgccgcgg tactcaccca
     6661 ggcgggccag gtcatcggca ggcccgaagt tggcgtcgtc gaacgggaag tccagcacga
     6721 cggtccaggc gccgctgcgc ccgcgcagcc ggtcgtcggt catttcccgc acgttttcgt
     6781 acagcagctc cacctcacgc ttggtgccgc gccaggtgan nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnc ccttgagcga ctccacttcg ggcaccagtg cacccagcag
     6961 caaggtcttc aacagccgcg cgtcgttgcg caggttcttg gccgcggtgg cgtcggcctg
     7021 tcccagctta atggcgtccc atgtcacacc atgctggcgt tccagcatcg gcagcatccg
     7081 ctgcgtgtac aagcgcttcg cgttttcaaa gtgcaggcgc atgccttcgc tgaacggctc
     7141 atcgccttcg gcgatggcgt cgtagaggtc gcccaccgga atcagctgtc ccagttccag
     7201 atcagcacgc cggtccacca gtagctgcag catcagcttg agcgccgtgc gctcgcgctg
     7261 cagcgcggcc gacacggcga tcagcgtttg caccagcgcg ggactgaacg gatagacctt
     7321 gcgaaacatg tcccggtcgg ccgtcgtcgt cagcagggtg tcgagcacat cccggcgcat
     7381 gttcaggatg ttgtcgaagg ccacctgcat cgtctggcgc gcggcttcgc tcaccggctt
     7441 caacacacgc ttctcggcaa tggccggcag gttgcgatct tccagcgtga tgcgatggaa
     7501 gcgtgcttcc cagtgcttca acacgtcgct gaactgcacc tgcaccacgc cagccaggtt
     7561 ctcgcccacc aggtcgcgca ggtcacgctg ccgcgccaca aagctcacca gaggaatggg
     7621 ccgatcggcg ttggtggcct ccaccagctt gaccagcttg gtgccctcgc ggctcacgaa
     7681 gttgacatcg gccgcatggc tggcgagcca cagcaccagt tcgtcgagga agaggatgac
     7741 cgcgtcatag cccaggtcac gcgcgtgccg gctcatgatg ctcaggccat cgtcgagcga
     7801 cacgtacgac tcaccgctgc cggcgatcgt gcggtacgcc gagaagaact ggctgatcag
     7861 gtcgcccacc aggcgcgaac gctcttcgcc gttcggcgcc tccagcatcg cggcttcaaa
     7921 gctggctgca tcccagccgc tgtcgaagga accccagccg ctggcaccgc cgctgccgcc
     7981 accttcatgt ttggcgcctt cgttcagctt ggcgaagaac gactcgtcgc ccatgcgcag
     8041 ccgcaacgca ctcgcgtcct tgaacaggcc ttcggccaga tagaagccgg gcaccggtgc
     8101 ctcggggtgc ttcttgcgca cgaactcggc gtactggccc agcacggcgc tttccatgtc
     8161 acgtgcaccg accatgtggt acggcaccag caggaacttg cggcctgtcg tccagccatt
     8221 acgtgccacc acgtcagcca gttcgggcgt ggcgcgggcc tgcgtattgc cggccagcag
     8281 caggttcagc acggccatga agtggctctt gcctgagccg aagctgccgt gcaggtacgc
     8341 cgccttgctg cccccggtct gaacggcctg ctggatgaaa cctagcgcgt tgctgaaggc
     8401 gtctaccagc tggggcgtca ccacgtagtc gcgcagggtc tggtcggcgt gggtgacgcc
     8461 ctcagacagc ttcagaacga agtcgccctg gtgcacccgc tctgggatgg cgatcaggtc
     8521 tctcagcagg gtcatggtcg cccctctccc atatgcttgt aggtcatcaa ttccgctctc
     8581 ggctgactca cggcaacgtc ggtaggccgg cacggttggc ggcggcgtgg agcgcctcct
     8641 tgaaccgatt gaagctcggc atgtcgatac gaagtggatc gacggcaccc gtcgccaccc
     8701 agtccgcctg ctcgtcggcc gacggcgcgt ttccgccgca cagggcctcc agctgacgct
     8761 tcagcgaatt tgggctcgcg tcgttgcccg acgcgtcttc cagtggcgca cagtttgcat
     8821 agcccggcgg gcgcagagcg cagagcagcc aagcttctga cttggggcgc ggcaccatcg
     8881 gcacgccgga ctcgcaaccg acgagctcaa agcctcgatg catggactcg accttccttt
     8941 gccaatcgtt ggctggcatc gcacgggtgc cgtcaccatc gcgaaaaagc acagccatca
     9001 ccggctggtc cccttcaagg gcggtctgat tcgcgaacag gcccaaagcc tgggcgttgc
     9061 gcgtgtagaa cgccatgccc ttcccgaact tcacgccagg aaacagtgga gaactcggtt
     9121 tgcccatcgc ggccagccgc gtttcgtgga cataggtcgc gcagtcagca cctgctagat
     9181 gggcttccag catgctgtaa gccgtgtggt gtcgctccag caacttgtcc accacccagg
     9241 ccatcgggcc gggcacaaac tccatcccgg ttgcggatgg tcgctcgtgg cccaggtcgg
     9301 tcggaccttc ccctgagaga accaatttca tggcgcctgc gtgtccaaca gcgccatggc
     9361 ctcagcgggc aatgcctcca ggtgcgtgtc gacgaagacc tcacccgggc caagcaactt
     9421 gagcttcttc tgggtgctgg gcaagtcaaa taagcgaatc accttggtgt ggccggccgt
     9481 gttgcggaac agcagcaaca ccgattcctt tgccacgtca tctggcaggt agttcagaac
     9541 caacgggctg tgtgtggtca cgatgacctg ccgcttggca tgcagcaaca gctcgacaag
     9601 cttctgcatc agttcggggt tgatgccgtt ctcgatctca tcgaacagca cgcagctctg
     9661 ccccaagccg gtgggctggt gggtgctgtc ctcaggctcg gacacgctca cttgggagag
     9721 cacagccaag acccgcagca ggccgtcgtt cacctgacga gcatccgtct ccaaacgggt
     9781 gggaaacaga tttgcgctgt cgtactgctc gacgatgcgc aggtccttcc atcctgcccg
     9841 cagcgccttg gtagccagtt cggcgacctg agggtagaag tgcttcaacg ctttggccaa
     9901 ctgggtcctg tccgcaggcg gcaagccatg gatgaacgcg ctgagccgct cgccgccgta
     9961 tccgatgtca gcggcgtctt tcgcgcgctt cctcatagat tgcggagaca ggagatcgag
    10021 cgaacgcaga ttggcggcaa cgtgcttcag cgcgcccaga gcctcgtgta catcgcgaac
    10081 gttgacgaaa gacagcgtcg agccctcgaa tttcatcgac ctcaaggggt actgaaccga
    10141 atccttggtg tcggtgccag ataccagcag ttgaccgtcg gagatgtgca gcacctgctc
    10201 cacgccgtcg agcgtgactg tctccgcggt gcagcgcatc agcgtggtgt tgtacgtgcc
    10261 gctccagaca atgtgtccga tgctcggaac gtcgacgcca agctcgaact tgatcaggac
    10321 cttcttcttc aggaactgac tcgttagatc cgcccgcttc cattcacgtt gcttgagcca
    10381 tgcgccgatc tgcccattgg ccagatgccc gatgaaatcg aaggcctgga gcaccgtcga
    10441 cttgcctgcg ccattcaaac ccaccaggca ggtgaacggc cccagttgct gtttggccgg
    10501 cggcagacgg aagtccacca acgacttgaa gttgtcgata tagaaacttc gaatcatggt
    10561 ttgaatcctt gaccggacct gtgcatcgag ttcttgattt ggtcgctcat gttgccacct
    10621 tggatttgcg gctacggtgc actggcgccg ccactgcctt tggctctcca gcgcgcgctt
    10681 cttcgcttcg ctcaggccac agcggaatct cgacaccggc cttgtcagcc agccatttca
    10741 ggctgtccgg catttgaatg gccatggtca gcagcagcgt ggtgctggcg tccgggtagg
    10801 tcgcctcgtt ttcgtaccgt gagaacgcaa tctttccctt gccgaagacg cgtgcggcgg
    10861 ccacttgtgt gaggccgtag cgcttgcgca ggcccacgat ctcctcgccc agcaacgtgt
    10921 tgccgtactg cgccttgcgg gcggccaacc ggcgcaggtt ctcgtcgtgc tgcggggcgc
    10981 gcgtggtctc cacgccgcag gcatcgcagc gcgagcccag cagctccacc gtcaccagcc
    11041 tgccgcgcgg cctgaagtcg cgagtccggg tgactgtatg cagctggccc gccttgcagg
    11101 cagggcaaac agattccgcg gttgtgtcag acacgatggt ggggcgggca aagcggccgg
    11161 aggctactgg cgaaaactta tcatcatgat aagtcctggc ctgtctcgac cggattccga
    11221 ctggcctgtc tgctcggagc gcacgtgttg gccaaacaaa cgtttcgcca gaacacgtta
    11281 aaaaacatag cctgttttta acgtgttgcg catccatggc ggctgaatgg ctcatcgcgt
    11341 cagcagcttg aacggcgcct gattgatgaa gtacacctcg cggccgatct tctggccgtg
    11401 cggcacgtcc aggcgctcca gctcgcgcaa gtacttggag caggtctggc gggtgcccat
    11461 ccccttcaac tcggccggcg cgtggctctt tgcgatagcc ctcttcagaa cggcagccgc
    11521 ttctacctcc gctgcgcgtg gcatggcggg caggtcattg aagggttggt cgcggtcaaa
    11581 cacgcatcac cctttcgatt ggcgagtgag gaactccagc gccgacggtg gaatgccttc
    11641 gtccttgacg atgccgtccg aactcggccg gaacaaggcc tgcaggatca actgcctgtc
    11701 ctccttgctt gcgaggcggt cgccctccag cagcgacagg taggtgcgtg ccatcaccac
    11761 gcgctctgcc gcgtcgccgg ccagatgcag gtggctgaga aacattcgaa caacaaggcg
    11821 tacaccccac accgcgaaca gaccgatcag caccagcatc gctacccgcc atgcttctgg
    11881 cgcagcgccg ggtgtcgcgg accgcagcag gtcatggatc agccagccca atccaccggc
    11941 tgcgcccgca atgcctgcga atgagatcgc gccggtgatc ttggacaagc ggtcatgctc
    12001 tttgcgtttg tcgtcccagt aggtggccgg tgcgcgcagc gcaatctctt cgcgaaacgt
    12061 ctttcgtagt ttctccatct cctccttgtg ttcggtgacc agcttctcga aatcaccgtc
    12121 ccgtttcgcc tgggcagcat cgaaatcggt cttctgctga tctgctgccg tcttgatcga
    12181 gtccgcgagt gcgctgtagt cccggtgcag cgcgtcgtag gccgtggtct tctcgccgag
    12241 cagcgcctcg gcctttacgc gcaactgctc gaacgattga tcagcagcct gcttgcggcc
    12301 cttcgcaggc gcgctagtca agcggtatct ctcaaccaag ccctcgatca gcccgcgcca
    12361 acccaccaac tcttgcggct ggaagtgatg gccttgctgc ggtggcacga aaacggatgc
    12421 gaagaagctg gctgggtggt ctccggcctc cttgcggtag tcctcaatgc gctttgcctg
    12481 cggcgttgag ctgtgaggca aatgctgcct aaggaacgct tcctccaggt gcgtccgaag
    12541 ctgtgagatt tggttctgat agtgctgagg gttcgagtcc ttgtactgct gcgcttggtt
    12601 tgccgtgttc tgcgcttgat tgagctgatt caaggcctgc ttgaggcctt ggtcatggtt
    12661 gccacccgcc cggctgttgt tgagccagga ccagaagttc acctcgtgct gggtccacga
    12721 gttcacctcg gccagactcg ttggcgcgaa gagccccccg ttgtccttca ggtccagcga
    12781 gaacagcggc tcggggcgtg gctctggcgt cgtgtttgtg ttcatcaggc ctccctgttc
    12841 gccttcttcc ggccgcgctt tgatgccacc actgcaggct tccaagcccg caggtcatcg
    12901 agcgtgaagc ccagctcccg agcatcctcg gtcacgaagc tgcggtagta ggtgcccatg
    12961 cgttgaccga actccgggtc caggtcgttg tgccactgct caagccaggg caccagttcc
    13021 agcaggccgg ccagcaaggg ttgcaggcga gcgggctccc agccttcgtt gttcttcatc
    13081 tcgccgtagt agccggccag cgcggtggcc agttgcaagt ggttccagcc ggcccaggcg
    13141 atgggcaggc tgccatcggc gccgcgctcg gcgccgggga agctgacaaa gcgttccttg
    13201 ggaacgtcta ggccgccgcg caggtgccaa agatcagccc gcaaaaagtc gctgctcttg
    13261 tatttgggtg gcgccgggat ggggccgacc tctcgtgcct tgcggtcctt gcggagggga
    13321 atgaaggtct tgtccagttc ctggcgcacc caggccaatg cgtctgcatc tggcgttgcg
    13381 cattccgcgg ctttggtgac caaggcaatg agttctaggc gtcgcgcttc ctccaactga
    13441 tcaatctcgg catcgatggc gtcttcgcgg cgctgcagga cccaggtgtc ctcccactgg
    13501 gctcgcttgc gcaggccatc gttcgtatac cgctgcacgg gtagcagcgg catagcttca
    13561 gcagctacca gttccgcaac tagccgggcg ggttcaaagt cggtatggcc ggcgtagagc
    13621 gcggccactt gcatgaactc agcgtcggcg cgcgccaact cggcgaggcg gttcgtcgtc
    13681 gtcagctgag gcggcacttc ggcgctgcag ggccagagcg caggcttctc tagtcggtcg
    13741 agcagccagt aacgcatagc gtcctgttcc aagtcctgcc aggcaggagt gttccaccgg
    13801 cgcttgtact cgggccgttc gatcaggccg atgttgcggt cggactcgat cagtgcaatc
    13861 cggcgctcga cgaccgcacg atagtcgggc ggccagtgct ttggcagttc ggtgatgggc
    13921 ctgctgccgt ggcgctcgaa ccagttggtg gtttcgatgc cagccgagac gcggcgggcc
    13981 atcacgatct cgaaggcgcg ctcaccaagc gcaagttcag gcggcgtggc gcattcaagt
    14041 tccgcccctt tgccggtcgc gggcagcagg tcgtagatgc gatagcagtg ccagtcaagc
    14101 tcttcctgga tgccaatcag ttctcccaag agccgctccg cctcaaggtg acttgaatct
    14161 aataccgcgc gattcaaaac ttcggcgcgc gcggcaatgg cggaaggcat caacgcctcg
    14221 cgagtcttgg caatggtgtc tgccttgcct gcgatcaccg ctgggagtga acttggcagc
    14281 ggaatcgcgc ctagaggcgt actggcatgt tcccccccgc gtcagaatag ttgtcgcctc
    14341 catagaacac ccccgggggc ggcgat
//
LOCUS       DAOORV010000091        14310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5184_length_14310_cov_7.223641, whole genome shotgun sequence.
ACCESSION   DAOORV010000091 DAOORV010000000
VERSION     DAOORV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_14310_cov_7.223641"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..351
                     /locus_tag="PKV56_12305"
     CDS             115..351
                     /locus_tag="PKV56_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68642.1"
                     /translation="MLAQPASRAGNGRWQVEVGNAVLRFVEARDGRGEGLAGIDLEVT
                     DPQSITAAATACGCAWDGDAVMVGGVRFSLQTSR"
     gene            complement(481..1632)
                     /locus_tag="PKV56_12310"
     CDS             complement(481..1632)
                     /locus_tag="PKV56_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX68643.1"
                     /translation="MLIYPLLFIALVVAMFWVLGMVWFFGALCIVVAACFWAATRLKG
                     QSLAAFDQPASERFSTGPSAELKTALASLARMSEVVQRAPRWRRLAKAREAMDQLFEG
                     RSFNASFTPADCAGVPAEWVLAPGADATRRTLYIHGGAFVVGSPKSHRTLTSAFSSIT
                     GGAVLAIDYRLMPEHPRMAGIEDCRSAYRWMLDNGPQGPQAARVVFVAGDSAGGNLTL
                     SLIAWVRDEGLREPDAAVALSPATDSTSASPSLKSNLATDPMLGPLFAPLAKVPRSML
                     LLFGWMQNRINPRDPLISPVYGDLSRLPPLLVQASESEMLLDDARRYVNRARAAGSPA
                     RLQTWDHMVHVWQIFNPELPEAHQALEEIRKFLATVPRRDDVAGQGLTR"
     gene            complement(1799..3403)
                     /locus_tag="PKV56_12315"
     CDS             complement(1799..3403)
                     /locus_tag="PKV56_12315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOX68644.1"
                     /translation="MDHLSGMDASFLHLETPETPMHVGSLMLFELPKGYEGDFYEVVK
                     AMIGRRMHLCSVLHRKLAQMPFELAEPVWIEDDDLDLDYHIRSLTLPRPGTMKQLHQL
                     VSRLHSTLLDRSRPLWEMVVIEGLQNGQVAFYSKAHHSGVDGKAGSEMAKVLYDVSPT
                     VREVPPPRRRPASSGYQLGVTELLQAAVENSARQYLKVAQLLPTAVKAVGAAGKAIAS
                     TRTPKGERSLNLGLAPKTIFNESITNQRSYSTWSVPLDDIKALGKRVGGTVNTVVMAI
                     CSGALRRFLKERDLLPKKSLIAMVPVSLRAADNDDMNNQVSAIRVDLATEIHDLPARF
                     KAIHASSESAKAVVKELKPVLGVDTPITGAPWLMTGIASLLGRSALAARMPAAGNVAI
                     SNVPGPPVPLYMAGARMLSFYPVSIPYHGSALNITVQSYAGSLDFGLIACRRVVSQEE
                     SYELIEHMKAALKEIEALPSIETSAEAVTAPAPPARVADAAAKAPAVKPARKSSPPGK
                     SVGAKKTAAAARRTAAARTPRKGTPR"
     gene            complement(3476..4312)
                     /locus_tag="PKV56_12320"
     CDS             complement(3476..4312)
                     /locus_tag="PKV56_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX68645.1"
                     /translation="MLTHVADRIRPLIDRSDPLSPPSRALLLLEIRAVWELGAFFAMA
                     PMLRLAPAGDGHPVLVLPGLAASDVSTQGLRAFLSDRGYAAHGWQLGRNAGPRPGVEA
                     ALQRRLAELHERHGRKVSIIGWSLGGIFAREVARRAPQQVRSVITLGSPFAGEPRASN
                     AWRVYEALSGLEVDAHPWREAMKQPPPVPSTAIFSRSDGIVSWQGCLERQSPTTENIE
                     VEGSHSGLGHHPAVLYAIADRLAQPEGAWRPFDRSGLRRFVYPDPHRDQHQPRAGGVG
                     RE"
     gene            complement(4316..5227)
                     /locus_tag="PKV56_12325"
     CDS             complement(4316..5227)
                     /locus_tag="PKV56_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX68646.1"
                     /translation="MTVEIPYTSASLAKANGIELCYDTFGDPSAPPLVLIMGLGTQMI
                     AWDEAFCGRLAARGHRVIRFDNRDIGLSTRFDAAGVPDVGAALMAAMMGRPVDAPYRL
                     HDLADDVAGLLDALGIESADVAGASMGGAVAQLMAIHHPQRVRTLTSIMATTGEPGLP
                     PPTPEAMAVLTKPTPIDKAAYLQSYRQTLKVLRSGSFPEDEALDFERAERNFTRGLNP
                     AGVARQLVAVLASGGRKRALANVRVPTLVLHGDVDPLVPLACGIDTAEAVPGARLCVV
                     EGMGHALPITMWPQIVDAIAAHANQDR"
     gene            5348..6418
                     /locus_tag="PKV56_12330"
     CDS             5348..6418
                     /locus_tag="PKV56_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOX68647.1"
                     /translation="MSPDAPSSAASPQIVYRTPPDPERRVYLAFRIHAVLATLAEEGI
                     ASEMALAGSGLDAKRLTDPDTRVSYRQLETVARNALRLSKDPAIALRAGRRMHITAYG
                     TYGYANLCAATVSDAIAFGVKYHRIAGSVVDMAFSTDGQNAVYAYEPLIWSDPSQDLY
                     RFAIEFALASHLTVIQDLVGKAAKPVRVSVAYPPPAHARAYRTRLQCRVDFLQPNNEM
                     AFDAAWIAKPLPLANPIAFASCSEMCDRLLGELSADGSLASEIRSALIMHPGRFPDAE
                     AMAEQLAMHPRALRRRLNTEKTSYRHLLAEVRSRLAIEYLRRTKMTSEEIANRLGYSD
                     AANFRRAFTSWTGKNPSDFRIR"
     gene            6466..7733
                     /locus_tag="PKV56_12335"
                     /pseudo
     CDS             6466..7733
                     /locus_tag="PKV56_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
     gene            complement(7781..8314)
                     /locus_tag="PKV56_12340"
     CDS             complement(7781..8314)
                     /locus_tag="PKV56_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX68648.1"
                     /translation="MREALPDDAIVTNGAGNYAVWLHRFFEYRSPRTELAPTCGAMGY
                     GLPAAIAAALRHPERQVVCVAGDGCFLMYSQELATAAQYGARLTVLVVNNGMYGSIRM
                     HQEKRYPNRVSGTQLASPDYVALARSFGAHAERVSRTEDFAGAFSRANAHVGLALLEF
                     VTDPQQILPITRIASSG"
     gene            complement(8480..8710)
                     /locus_tag="PKV56_12345"
                     /pseudo
     CDS             complement(8480..8710)
                     /locus_tag="PKV56_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            8731..9057
                     /locus_tag="PKV56_12350"
     CDS             8731..9057
                     /locus_tag="PKV56_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68649.1"
                     /translation="MAPNAKLRPLVVPQGSEVQKLATDVTVAGECAVQTVQARPQRIS
                     WARLLKRVFDIDMQHCPNCGAGELKIIAAILEWPVIGKILTHLGLYPQPPPRGRAHEA
                     GQDFVT"
     gene            9374..12562
                     /locus_tag="PKV56_12355"
     CDS             9374..12562
                     /locus_tag="PKV56_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOX68650.1"
                     /translation="MPRTEPIPQPKGDPFIGNMRAIDGDAPMQGFMRLARIHGPIFQL
                     EFFGKPLILVSSQEIVNELCDEKRFDKRVHATLKNIRDFAGDGLFTARTEEPNWGKAH
                     RLLMPAFGPLGIRGMFDQMLDIADQMLMRWERFGPDNVIEVTEDMTRLTLDTIALCAF
                     DYRFNSFYQREMHPFVGAMVEALSESGARERRPDIANKLMLRTRRAYEDNLHMMREVA
                     DGLIAERKRDPNGASKKDLLGLMLQGRDPVTGEGLSDENIRYQLVTFLIAGHETTSGL
                     LSFATYFLCKNPQVLAKARAEVDAAFGNEMPRIEHLAKLRYIEQILMETLRIWPTAPA
                     FALKPYEDTLLAGRYALRKWDTVMVLVPMLHRDTKVWGNDVEAFRPERFEPDTFAAMP
                     PNSWKPFGIGQRACIGRPFAMQEAQLLLAMMLQRFDLIEDDPSYQLQVSETLTLKPHG
                     FRIRVKPRGSASFKARSAAAPATTAKPAQTPAAAKTAPVGQTTPLLVLYGSNTGSAEA
                     FAERIASDAPQQGYAVQVAPLDEHTGRLPTDGAVVLLTASYEGQPPDNARQFAAWLDS
                     QGADALKGVRYAVFGCGNRQWARTYQAVPKRFDAALEAAGATRIKDRGETDSGGDFFG
                     AFDDWYAGLWDELGKSLGKEVKAAPTGGQLDVEIVKTGRTSILRLAELEHGEVVENRE
                     LVDMKSPLGRSKRHIDIRLPEGMSYRAGDYLAVLPHNPIGVVMRALKRFGLASDSQVV
                     ISKPETSITSLPVGYPVTASELLFHYVELAQPATRAQVGQLAVATRCPPEKAELDQLA
                     DDAAYQQQVLGKRLSVLDLLERFASCELSFGAFLAMLPPTKARQYSISSSPLWDANRC
                     TLTVAVVDAPAISGQGRYQGMASSYLANCSPGTKVAVVVRPSNEHFHPPADPQTPMVM
                     VCAGTGLAPFRGFLQDRAVQAANGQKVGPALLFFGCDHPDVDHIYKDELAGWEAAGVV
                     SVRPAYFKAPQGEVTFVQHRVWQDRADIADLFRKGANVYVCGDGKRMAPAVRDTFIRI
                     YREAVGVTAETAEAWFDKVERDTGRYVSDVFA"
     gene            12596..13627
                     /locus_tag="PKV56_12360"
     CDS             12596..13627
                     /locus_tag="PKV56_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX68651.1"
                     /translation="MATPTITEHQTRSDRHTTHYLACGPADGTPVIFLHGWPDLALGW
                     RHQLLALGALGFRALAPDMRGYGQSTVHPAKTDYALEACVADMLELLASTGREAAVWV
                     GHDLGAPVAWAIASHHPERTLAVAALCVPYMPQGMSPPKLVELVDRSVYPITEFPVGQ
                     WDYQLHYLENFDHAVAELGANPVNTVKAMFRAGSPAHAGKPSPTALLRKRGGWFGKAG
                     AAPDLPLDRSVMSNDELRHYADALARNGFAGPNSWYTNGERNDAYMQASVNGGRLAMP
                     ALFIHASYDAICETLKSPLAGPMRAHCARLTELTVDAGHWAAQEQSERVNAGLARWLA
                     NDLAGAWRC"
     gene            complement(13729..13917)
                     /locus_tag="PKV56_12365"
     CDS             complement(13729..13917)
                     /locus_tag="PKV56_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOX68652.1"
                     /translation="MGKRRVPSLPDLPTIAEQGFADVDKAGWFAVVAPKRLPAAQFVK
                     TEQDRCAQLVKKAGITME"
     gene            14069..>14310
                     /locus_tag="PKV56_12370"
     CDS             14069..>14310
                     /locus_tag="PKV56_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOX68653.1"
                     /translation="MPLAVPSTQRDQALALLRRRGLTRLAELRSAGVTGTTVSRMERA
                     GEVVRLARGLYQLPNAALDPQQSLAEASRLVPKGVI"
BASE COUNT         2368 a         4860 c         4827 g         2255 t
ORIGIN      
        1 ccgcaggagc ctggggatcg ccgcttaacc agtccaagtt tttggccgca ccccccctga
       61 tggtcagttt tcggtcggcg ccaacacgaa cgactggcca ggcgctgggg cgaggtgctc
      121 gcccaaccag ccagccgcgc tggcaatggg cgatggcaag tggaggtcgg caacgcggtg
      181 ctgcgctttg tcgaagcgcg cgacggccgc ggcgaagggt tggccggcat cgacctggaa
      241 gtcaccgatc cgcagtccat cacggcggct gcgactgcct gcggctgtgc ctgggacggc
      301 gatgccgtga tggtcggcgg cgtgcggttc agcctgcaga cgagccgcta gtgtccagtg
      361 ccatctcgaa gagggcatct gtctggcgca caacgccgag ggtcggcgcg tgttcgtcag
      421 cgatctggca gcccctctgc gcgaggtgga aatcaagcgc ccagtgggcc gcgctgaagg
      481 tcagcgcgtc agcccctggc cagcgacgtc gtcgcgccgt ggcaccgtcg cgaggaactt
      541 gcgaatctct tccagcgcct ggtgagcctc gggcagctca ggattgaata tttgccagac
      601 gtggaccatg tggtcccagg tctgcagccg cgccggtgag ccggccgctc gcgcgcggtt
      661 gacatagcgc cgggcgtcgt cgagcagcat ctccgactcg ctggcctgca ccagcagcgg
      721 cggcagccgc gacaggtcgc cgtagaccgg cgaaatcagc gggtcgcgcg ggttgatgcg
      781 gttctgcatc cagccgaaca gcagcagcat cgaccgtggc accttcgcca gcggcgcgaa
      841 cagcgggccg agcatcgggt cggtcgccag gttggatttc aggcttgggc tggcgctggt
      901 gctgtcggtg gcgggcgaca gcgccaccgc ggcatcgggt tcacgcaagc cctcgtcgcg
      961 cacccaggcg atgagcgaca acgtgaggtt gccgccggcc gaatcgccag cgacgaacac
     1021 cacgcgcgcc gcctgtggac cttgcgggcc gttgtcgagc atccagcggt aggcgctgcg
     1081 gcagtcctcg atgccggcca tgcgcggatg ctccggcatc agccggtagt cgatcgcgag
     1141 cacggcgccg ccggtgatcg acgagaaggc gctcgtcagc gtgcggtggc tcttcgggct
     1201 gccgacgaca aaggcgccgc catggatgta gagcgtgcgc cgggtggcgt cggcaccagg
     1261 cgcaagcacc cattcggcgg gcacgccggc gcagtcggct ggcgtgaagc tggcgttgaa
     1321 actgcgacct tcgaacagct gatccatcgc ctcgcgcgcc ttggccaagc gccgccaacg
     1381 aggcgcacgc tgcacgacct cgctcattcg tgccagcgac gccagcgccg ttttcagttc
     1441 ggcgctcggc ccggtcgaga aacgctcgct ggccggctgg tcgaaggccg ccaggctctg
     1501 ccctttcagg cgggtggcgg cccaaaagca ggccgcgacg acgatgcaga gcgcaccgaa
     1561 gaaccagacc attccgagaa cccagaacat ggcgacgacc aacgcaatga agagcaaagg
     1621 gtagatcagc atgggcattc aggcctcgac cgcaggtctc agttctgtcc ccaccgccgc
     1681 tggccggccg ggcaggatca gcgccgaccc cgttgccaac gcgacggtct tggagctgcg
     1741 gtgcgggtgc gggtgctgga cactgcgcgt gcacggcgtc gcgtcctgct ccggcggttc
     1801 accggggtgt tcccttgcgc ggcgtgcggg ccgctgcggt gcgtcgcgcc gcagcggctg
     1861 tcttcttcgc gccgaccgac ttgccgggtg gcgatgactt tcgagcgggc ttgacggctg
     1921 gcgccttcgc cgccgcatcg gcgacgcgcg caggcggggc cggcgcagtc acggcctcgg
     1981 ccgacgtctc gatcgacggc agcgcctcga tctccttcag cgccgccttc atgtgctcga
     2041 tcagttcgta cgactcctcc tgcgacacca cgcgcctgca cgcaatgaga ccgaagtcga
     2101 gcgaaccggc ataactctgc acggtgatgt tcaaggccga gccatggtaa ggaatcgaca
     2161 cggggtagaa gctcaacatg cgcgcacccg ccatgtacaa cggcaccggg ggccccggca
     2221 cgttggagat ggcgacgttg cccgccgccg gcattcgcgc cgccagggcc gagcggccga
     2281 gcagcgacgc gatcccggtc atcaaccacg gtgcgccggt gatcggcgta tccacaccga
     2341 gcacgggctt cagttccttc accaccgcct tggccgactc ggacgaggcg tggatcgcct
     2401 tgaatcgcgc cggcaggtcg tgaatctcgg tcgccagatc gacgcggatc gccgacacct
     2461 ggttgttcat gtcgtcgttg tcggctgcgc gcaggctgac cggcaccatc gcgatcagcg
     2521 acttcttcgg cagcaggtca cgctccttca gaaagcggcg cagcgcgccg ctgcagatcg
     2581 ccatcaccac ggtgttcacc gtgccgccga cgcgcttgcc gagcgccttg atgtcgtcca
     2641 gcggcaccga ccaggtgctg tacgagcgct ggttggtgat cgattcgttg aagatcgtct
     2701 tcggcgccaa accgaggttg agcgagcgct cgcccttcgg cgtgcgggtg ctcgcgatcg
     2761 ccttgcccgc cgcaccgacc gccttcaccg ccgtcggcaa cagctgcgcg accttgaggt
     2821 actgccgcgc cgagttctcg acggctgctt gcagcaactc ggtgacaccg agctggtagc
     2881 cgctgctggc cggccggcgc cgcggtggcg gcacctcgcg caccgtgggc gagacgtcgt
     2941 agagcacctt ggccatctcg gagcccgcct tgccgtcgac gccgctgtga tgcgccttgc
     3001 tgtagaaggc gacctgcccg ttctgcaggc cttcgatcac gaccatctcc cacagcgggc
     3061 gactgcgatc gagcagggtc gagtgcagtc gcgagacgag ctggtgcaac tgcttcatcg
     3121 tgccgggccg gggcagggtc aaactgcgga tgtgatagtc gaggtcaagg tcgtcgtcct
     3181 cgatccatac gggctcggcg agctcgaatg gcatttgcgc cagcttgcga tgtagcaccg
     3241 agcacaggtg catgcggcgg ccgatcattg ccttcactac ttcgtagaag tcgccctcgt
     3301 atcccttggg cagctcgaac aacatcaggc tgccgacgtg catcggcgtc tcgggggtct
     3361 cgaggtgcaa gaacgatgca tccatgccgc tcaggtggtc catcgtcgtc tccttgttcg
     3421 aggaaattgg ggggctagcc gcggaccgtc gggccaggac tggcgccact gcgcgtcatt
     3481 cccggccgac tccacccgct ctgggctggt gctgatctcg atgcggatcg ggatagacga
     3541 agcgtcgcag accgctgcgg tcaaagggcc gccatgcgcc ttcgggttgc gccagccggt
     3601 cggcaatcgc gtacagcacc gcagggtggt ggccgagtcc gctgtggctg ccctcgacct
     3661 caatgttctc ggtcgtcggg ctttggcgct ccaggcatcc ctgccaggag acgatgccgt
     3721 cgctgcggct gaagatcgcc gtgctcggca ccggcggcgg ttgcttcatc gcttcgcgcc
     3781 agggatgcgc atcaacctcg aggccgctca gcgcctcgta gacccgccag gcattgctgg
     3841 cccgtggctc gcctgcgaac gggctgccga gcgtgatgac gctgcgcacc tgctgcggag
     3901 cccggcgtgc gacctcgcgc gcgaagatgc cgccgagact ccagccgatg atgctgacct
     3961 tgcggccgtg gcgctcgtgc agctcggcca gccgccgctg caacgctgct tccacgcccg
     4021 gacggggtcc ggcattgcgc ccgagctgcc agccgtgggc cgcgtagccg cggtcactca
     4081 ggaaggcgcg caagccttgg gtggacacat cgctggcggc gagccccggc agcacgagca
     4141 ccgggtgacc gtcgccggcc ggcgccagcc gcagcatcgg cgccatggca aaaaaggcac
     4201 ccagttccca taccgcccgg atctcgagca gcagcagcgc acgcgacggc gggctcaacg
     4261 ggtcggaacg gtcgatcagg ggtcgaatgc ggtcggcaac gtgtgtgagc atagttcatc
     4321 gatcctggtt ggcgtgcgcc gcgatggcgt caacgatttg cggccacatg gtgatgggca
     4381 acgcgtggcc catgccttcg acgacgcaca ggcgcgcgcc tggcacggct tcggcagtat
     4441 cgatgccgca agccaatgga accagcggat cgacgtcgcc gtggagcacg agcgtgggca
     4501 cccgcacgtt ggccagcgcg cgcttgcgcc cgccggacgc gagaaccgcc accaattgcc
     4561 gcgcgacgcc ggccgggttc aaccctcgtg tgaagttgcg ctcggcacgc tcgaagtcga
     4621 gtgcctcgtc ctccgggaag ctgccactgc gcagcacctt caaggtctgc cggtagctct
     4681 ggagataggc cgccttgtcg atcggggtcg gcttcgtcag gaccgccatc gcctcgggcg
     4741 tcggtggcgg caggccgggc tcgccggtgg tcgccatgat cgaggtcagc gtgcgcacgc
     4801 gctgtggatg gtggatggcc atgagctgag cgactgcgcc gcccatcgac gccccggcga
     4861 catcggccga ttcgatgccg agcgcgtcga gcaggcctgc cacgtcgtcg gccaggtcgt
     4921 gcagtcgata tggcgcgtcg accgggcgcc ccatcattgc ggccatcagc gccgcaccga
     4981 catccggcac gccggccgca tcgaagcgcg tcgacagacc gatgtcgcgg ttgtcgaagc
     5041 ggatcacgcg atggccgcgc gcggccaacc tgccgcagaa cgcctcgtcc caagcaatca
     5101 tctgcgtccc caggcccatg atcagcacca gcggcggtgc agatgggtcg ccgaacgtgt
     5161 cgtaacacag ctcaatgccg ttggccttgg cgagcgatgc tgacgtatac ggaatctcta
     5221 ccgtcatcgt gtctcctcga tggcggattc ttgggttcgt ggacacggca cggaaggtcc
     5281 gtttggtgca ctcagacagt cacttttgga cagcgcccgc cagcggcgct aagctcgcgc
     5341 gctacatatg tcgcctgatg cgccgagcag cgccgctagc ccgcagatcg tctatcgcac
     5401 gccgcccgac cccgagcgcc gcgtgtactt ggcgtttcgt attcacgcgg tgcttgccac
     5461 gctcgccgaa gaagggatcg cgagcgaaat ggccctcgcg ggcagcgggc tcgacgcgaa
     5521 acgactaacc gatcccgaca cgcgcgtgtc gtatcgtcag ctcgagaccg tcgcccgcaa
     5581 tgccttgcgc ctctcgaagg acccggccat tgcgttgcgg gcgggccgac gcatgcacat
     5641 caccgcctac ggcacctatg gctatgcgaa cctgtgtgca gcgaccgtca gtgacgccat
     5701 tgccttcggg gtcaagtacc accgcattgc tggttcggtg gtggacatgg cgttttcgac
     5761 tgacggccag aacgctgtct acgcgtacga accgctgatc tggtcggacc cgagccagga
     5821 tctgtaccgc ttcgcgatcg agtttgctct cgcgagtcac ctgacggtga tccaggatct
     5881 tgtcggcaag gctgccaaac cggtgcgcgt gagcgtggcc tacccccccc cggcacacgc
     5941 acgcgcctac cgcacccggc tccaatgccg cgtggatttt ctgcagccca acaacgagat
     6001 ggcattcgac gcggcctgga tcgcgaagcc gttgccgctg gccaacccga tcgccttcgc
     6061 ttcgtgcagc gaaatgtgcg atcgcctcct gggcgaactg agcgccgacg gcagcctcgc
     6121 ctccgaaatc cgcagcgcgc tgatcatgca ccctggccgc ttcccggacg ccgaggcgat
     6181 ggccgagcag ttggcgatgc atccgcgcgc attgcgccgc cggctcaaca ccgagaagac
     6241 ctcgtaccgt cacctgctcg ccgaggtgcg gtcgcgcctc gcgatcgagt acctgcgccg
     6301 gaccaagatg accagcgaag agatcgcgaa ccggctcggc tacagcgacg ctgcgaactt
     6361 ccgccgcgca ttcacgtcgt ggactggcaa gaacccgtcg gacttccgga ttcgctaggg
     6421 acgtgccgag ggcgacgttc cgaacacacg acgaggaggg acatcatgcg tgtgatcgtt
     6481 ctcggcgccg gcctgctcag tgtcacgtct gcctactacc tgcaaaggct tggccacgag
     6541 gtgaccgtga tcgaccggca ggcctccccg gcggccgaga ccggctttgc caacggcggg
     6601 cagatttccg tcagccatgc cgagccttgg gccaacccga gcgtgccgct gaaggtgctg
     6661 cagtggctcg gcaaggagga cgcgccgctg ctgtttcgca ttcgcgcaga catgcggcaa
     6721 tggctttggg gcctgcagtt ccttcgagag tgcacgccgg cgcgcacgtg ccacaacatc
     6781 gcgcagatcg tgcggctggg tacctacagc cgcgagatgc tgcaggcact gcgccacgag
     6841 gcgggcatca gctaccacga gcgcacccag ggcatcctgc acctctatac gacgcagaag
     6901 gaatttgacg gtgcgttgaa gccggccgag cagatgcgcg cactcggctg cgagcgccag
     6961 gtgatctcgg ccgacgaggc cgtcaagatc gagtcggcgc tgtcccacat ccgtccgcaa
     7021 ttggctggcg ccacctacac ggccgaagac gaatcgggtg acgccaaccg cttcgcgcgc
     7081 gaactggtca agctctgcga agcggccggc gtcaggttct cgatgagcca caccgtcacc
     7141 gcgatccgcg aagccggggc caggtcgagc atgtcgaggc gaccgacaac gaaggccgct
     7201 tccagcgcgt cacgggcgat gcctatgtgc tggcgatggg ctcgcacagc ccgttctacg
     7261 gcgcgccgct gggcatccgg ctgccgatct acccggccaa gggctactcc gtcacgatgc
     7321 cggtgatcga cgcatcccga gcgcaccagg tctcgctgac cgacgacgaa ttcaagctcg
     7381 tggtctcgcg ctacaccgac gaagccggcg accgcctgcg catcgccggc acggccgaac
     7441 tcaacggcta cgaccgcgac ctgaaccgtg tgcggtgcga ggccatcgtg cgccgcgtcg
     7501 agcagctctt ccccggcgcc ggtgacgcca cgcgtgctca gttctggacc gggctgcgcc
     7561 ccgcgacgcc gagcaacgtg ccgctgatcg gcaagaccaa gctgccgaac tttttcctca
     7621 acaccggcca tggcacgctg ggctggacgc attcgtgcgg ttcgggcaag tcgatcgccc
     7681 gcatcgtgag cggtctggcg ccggaagtgg actttgcgtt cacgggcgcg taggccacgg
     7741 ccatcgcgcg accgggcgag tcgacgggtt cgcagctgac tcagcccgac gacgcgatgc
     7801 gtgtgatcgg cagtatctgt tgggggtcag tgacaaactc cagcagcgcc aagccaacat
     7861 gcgcattcgc gcgactgaac gcgcctgcga agtcctcggt tcgtgacacc cgctccgcgt
     7921 gcgcaccgaa tgatcgggcc agcgcgacat agtccgggct cgccagctgc gtgccgctga
     7981 cccggttcgg gtagcgcttc tcctggtgca tgcggatgct cccgtacatc ccgttgttga
     8041 cgaccagcac cgtgagccgg gcgccgtact gcgcggcggt tgccagttcc tgcgagtaca
     8101 tcaggaagca gccgtcgccg gcgacacaga cgacctggcg ctcggggtga cgcagcgcgg
     8161 ccgcaatggc ggccggcaag ccgtagccca tcgcgccgca ggttggcgcg agctcggtcc
     8221 ggggtgaccg gtactcgaag aaccggtgca gccagacggc atagttgccc gcgccgttcg
     8281 tcacgatcgc atcgtcgggc agcgcctccc gcatctgatg catggccgcg gcgaggtcca
     8341 cgccggtggc ccgcgagtcc cgcggcggca actcgccgaa gcctagaaag gcctcccgag
     8401 ccgactgcgt ccaaaggtcc cacgccctcg catcgggcgg ggccagcgca accagcgacg
     8461 cggaggctgc ggccacgccg gcctcgaaac ccatctcggg acgaaacacg cggccgacct
     8521 cgtgcgcctc gggacggaag tggatgagca gctgttcggg gttcggtgcc aagatcaacg
     8581 tgtagctgtc gctcgcgacg tcggtgatcc tggtgccgac ggcgagcacg acatcggcgg
     8641 tgcgcagccg ctcggcgagc gccgggttca tgccgagact gaggtgccct gcatactgcg
     8701 gcagccggtt gtcaaagaga tcctggtgtt atggcgccca acgccaagct gcgcccgctg
     8761 gtggtgccgc agggatccga ggtgcaaaag ctggccaccg atgtcacagt tgccggcgaa
     8821 tgcgcggtcc agacggttca ggcccggccg cagcgcatca gctgggcgcg gctgctcaag
     8881 cgcgtgttcg acatcgacat gcagcactgc ccgaactgcg gcgccgggga actgaagatc
     8941 atcgccgcca tcctcgagtg gccggtcatc gggaagatcc tcacccacct ggggctatat
     9001 ccgcagccgc cgcccagggg ccgagcgcac gaggccggac aagatttcgt cacctgagtc
     9061 gcgccggccg tcacaaaaac cacgccccag gctgcgacgc cagaccgcag cctgggcggc
     9121 gtcgtgcggc gcgccagcgc agcgccgtcg gaagcagggt caaccccgag agcttggtca
     9181 ccagcgagcc ttgtgagcgc tgaagggact tccgaaccag cattgcgcga cgtcacggcc
     9241 gcagaatcca ggcgcctctg acagccgcag gccagtcagg ccagtcacgc gtcgaacccg
     9301 agggcgtttg gaagtactat gccccacggc caagcgaccg acgccacgac aaatcgccac
     9361 ccaggagacc cgcatgcccc gcaccgagcc cattccgcag cccaagggcg acccgttcat
     9421 cggcaacatg cgcgccatcg acggcgacgc gccgatgcag ggcttcatgc ggctggcccg
     9481 catccatggc ccgatcttcc agctcgagtt cttcggcaag ccgctgatcc tggtcagctc
     9541 gcaggagatc gtcaacgagc tgtgcgatga aaagcgcttc gacaagcggg tgcatgccac
     9601 gctgaagaac atccgcgatt ttgccggcga cggcctcttc accgcgcgca ccgaagagcc
     9661 caactggggc aaggcgcacc ggctgttgat gccggcattc ggcccgctgg gaatccgcgg
     9721 catgttcgac cagatgctcg acatcgccga ccagatgctg atgcgctggg agcgcttcgg
     9781 ccccgacaac gtgatcgagg tcaccgagga catgacccgc ctcacgctcg acacgatcgc
     9841 gctgtgtgca ttcgactacc gcttcaacag cttctaccag cgcgagatgc acccgttcgt
     9901 gggcgcgatg gtcgaggcgc tgtccgaatc gggcgcgcgc gaacggcggc ccgacatcgc
     9961 caacaagctg atgctgcgca cgcgccgggc ctacgaggac aacctgcaca tgatgcgcga
    10021 ggtggccgac ggcctgatcg ccgagcgcaa gcgcgacccc aacggcgcca gcaagaaaga
    10081 cctgctgggc ctgatgctgc aaggccgcga cccggtgacc ggcgagggcc tgtcggacga
    10141 gaacatccgc taccagctgg tcaccttcct gatcgccggc catgaaacga ccagcggcct
    10201 gctgtcgttt gcgacctatt tcctgtgcaa gaacccgcag gtgctggcca aggcgcgggc
    10261 cgaggtcgat gccgcattcg gcaacgagat gccgcgcatc gagcacctgg ccaagctgcg
    10321 ctacatcgag cagatcctga tggagacgct gcgcatctgg cccaccgcgc cggcgtttgc
    10381 gctcaagccc tatgaagaca cgctgctggc cgggcgttac gcgctgcgca aatgggacac
    10441 ggtgatggtg ctggtgccga tgctgcaccg cgacaccaag gtctggggca acgatgtcga
    10501 ggcgttcagg cccgagcgct tcgagcccga cacctttgcc gcgatgccgc ccaattcatg
    10561 gaagccgttt ggcatcggcc agcgcgcctg catcggccgc ccgtttgcca tgcaggaggc
    10621 gcagctgctg ctggcgatga tgctgcagcg ctttgacctg atcgaggacg atccgtcgta
    10681 ccagctgcag gtgtcggaga cgctgacgct caagccccat ggctttcgca tccgcgtcaa
    10741 gccgcgtggc agcgcatcgt tcaaggcccg cagcgcggcc gcaccggcca ccacggccaa
    10801 gccagcgcag acacctgccg cggccaagac agcgccggtc ggccagacaa cgccgctgct
    10861 ggtgctgtac ggatcgaaca ccggatcggc cgaagccttt gccgagcgca tcgccagcga
    10921 cgcgccgcaa cagggttatg cggtgcaggt ggcgccgctg gatgaacaca cgggccggct
    10981 gccgaccgac ggcgcggtgg tgctgctgac ggcatcgtat gaaggccagc cgcccgacaa
    11041 cgcacgccag tttgcggcct ggctcgacag ccagggcgcc gatgcgctga agggtgtgcg
    11101 ttatgccgtc ttcggctgcg gcaaccggca atgggcgcgc acctaccagg ccgtgcccaa
    11161 gcgctttgat gccgcgctgg aggcagccgg cgccacgcgc atcaaggacc gtggtgagac
    11221 cgattcgggc ggcgacttct tcggcgcctt cgatgactgg tatgccggcc tgtgggacga
    11281 actgggcaag tcgctcggca aggaggtcaa ggccgcgccg accggcggcc agctcgatgt
    11341 cgagatcgtc aagaccggtc gcacgtcgat cctgcggctg gccgagctcg agcatggcga
    11401 ggtggtcgag aaccgcgagc tggtcgacat gaagtcgccg ctgggccgct cgaagcgcca
    11461 catcgacatc cgcctgcccg agggcatgag ctaccgtgcc ggcgactatc tggccgtgct
    11521 gccgcacaac ccgatcggcg tcgtgatgcg cgcgctcaag cgcttcggcc tggcgtcgga
    11581 cagccaggtg gtgatcagca agcccgagac cagcatcacc tcgctgccgg tgggctatcc
    11641 ggtgacggcc agcgagctgc tgttccacta tgtcgagctt gcccagccgg ccacccgggc
    11701 ccaggtcggc cagctggccg tcgccacccg ctgcccgccc gaaaaggccg agcttgacca
    11761 gctggccgac gacgccgcct accagcaaca ggtgctgggc aagcgcctga gcgtgctcga
    11821 cctgctggaa cggtttgcgt cgtgtgagct gagcttcggc gccttcctcg cgatgctgcc
    11881 gccgaccaag gcgcggcagt attcgatctc gtcgtcgccg ctgtgggatg ccaaccgctg
    11941 cacgctgacg gtggcggtgg tcgatgcgcc ggccatctcg ggccagggcc gctaccaggg
    12001 catggcctcg tcgtacctgg ccaattgctc gccgggaacc aaggtggcgg tggtggtgcg
    12061 gccgtcgaac gaacacttcc acccgccggc cgacccgcag acgccgatgg tcatggtctg
    12121 cgccggtacc gggctggcgc cgttccgcgg cttcctgcaa gaccgcgcgg tgcaggccgc
    12181 caacggccag aaggtgggcc cggcgctgct gttcttcggc tgcgaccatc ccgatgtcga
    12241 ccacatctac aaggatgagt tggccggctg ggaggcggcg ggcgtggtca gcgtgcggcc
    12301 cgcgtacttc aaggcgccgc agggcgaggt gacgtttgtg cagcatcggg tctggcaaga
    12361 ccgcgccgac atcgccgacc tgttccgcaa gggcgccaac gtgtatgtct gcggcgacgg
    12421 caagcgcatg gcgcctgccg tgcgcgacac cttcatccgc atctaccggg aggcggtggg
    12481 cgtgacggcc gagaccgccg aagcctggtt cgacaaggtg gagcgcgaca ccgggcgtta
    12541 tgtgtcggat gtgtttgcct gaccggcgga cctgaacacg ggagcttgca gcgatatggc
    12601 cacaccgacg atcaccgagc atcagacgcg ctcggaccgc cacacgacgc attacctggc
    12661 ctgcgggccg gccgacggca cgccggtcat cttcttgcac ggctggcccg acctggcgct
    12721 cggctggcgg caccagctgc tggcactcgg cgcgctgggc tttcgtgccc tggcccctga
    12781 catgcggggt tatggccagt cgaccgtgca tccggccaag accgactatg cactcgaagc
    12841 ctgcgtggcc gacatgctgg aactgctggc ctcgaccggg cgcgaggcgg cggtctgggt
    12901 cggccatgac ctgggcgcgc cggtggcctg ggcgatcgcg tcgcaccatc ccgagcgcac
    12961 gctggcggtg gccgcgctgt gtgtgcccta catgccgcag ggcatgtcgc cgccgaagct
    13021 ggtcgagctg gtcgaccgca gcgtctaccc aatcaccgag tttccggtcg gccagtggga
    13081 ctaccagctg cactacctgg aaaacttcga ccatgctgtg gccgaactgg gcgccaaccc
    13141 agtcaacacg gtgaaggcga tgttccgcgc cggaagcccg gcccatgcgg gcaagccatc
    13201 gccgaccgcg ctgctgcgca agcgtggcgg ctggttcggc aaggccggcg cggcgcccga
    13261 tctgccgctc gaccgcagcg tgatgagcaa cgacgagctg cggcactatg ccgacgcgct
    13321 ggcacgcaac ggctttgccg gccccaacag ctggtacacc aacggcgagc gcaacgatgc
    13381 ctacatgcag gcgtcggtga atggtggccg gctggcgatg ccggccctgt tcatccacgc
    13441 cagttacgac gcgatctgcg agacgctgaa atccccgctg gccgggccga tgcgcgccca
    13501 ttgcgcgcgg ctgaccgagc tgacggtcga tgccgggcat tgggcggcgc aggaacaatc
    13561 cgagcgggtg aatgcggggc ttgcacgctg gctggcaaac gacctggcgg gggcgtggcg
    13621 gtgctgaatg ggcaggggcc gggcggtgcg gatccggccg gcgcctgatg gtgcggatcc
    13681 cggcggcgcc tggcaccggt gatcgggccg gcgctgacga tggacgcctc actccatcgt
    13741 gatgcccgcc ttcttgacca gctgcgcaca gcggtcctgt tcggtcttga cgaactgggc
    13801 cgcgggcagg cgtttgggcg caaccacggc aaaccagccc gccttgtcaa catcggcaaa
    13861 gccctgctcg gcgatcgtcg gcagatcggg cagcgatggc acacgccgct tgcccatgat
    13921 gcgttgtact ggtccggctt ggccaagggg tgccgcagac acaatcggcg tcgggtgatt
    13981 tgccgggcct gtgcggaacc agttggcgcg agcaagggga tggtataaat catcggacga
    14041 ttacatcgat catccgagcc ttcagaacat gccccttgcc gtcccctcga ctcagcgcga
    14101 ccaggccctg gcgctgcttc ggcggcgcgg cctgacgcgg ctcgcagagc tgcgcagcgc
    14161 aggcgtcaca ggcaccacgg tgagccgcat ggagcgcgcc ggagaggtcg ttcgcctggc
    14221 ccgcgggctg taccaactgc ccaacgccgc gctcgaccca caacagtcgc tggccgaagc
    14281 atcacgcctc gtacccaagg gcgtgatctg
//
LOCUS       DAOORV010000092        14164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5250_length_14164_cov_8.865547, whole genome shotgun sequence.
ACCESSION   DAOORV010000092 DAOORV010000000
VERSION     DAOORV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_14164_cov_8.865547"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKV56_12375"
     CDS             complement(<1..433)
                     /locus_tag="PKV56_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOX68654.1"
                     /translation="MGNKLYVGNLSYNVRDDDLQQTFAQFGTVSSAKVMMDRETGRSK
                     GFGFVEMGSDAEAQSAINGMNGQQMDGRALVVNEARPREERPGGFGGGGSRGGFGGGG
                     GGGGGGGGFGGGAGRSGGGGGGGGGGGGGGGGGGGGGGALCP"
     gene            694..1332
                     /locus_tag="PKV56_12380"
     CDS             694..1332
                     /locus_tag="PKV56_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOX68655.1"
                     /translation="MDILKPLIALLAVVNPIGVVPFFLHFTQNFTPEQRRRTIRVSAV
                     SAFVVIAVSALAGLQIIQFFGISLASFQVGGGLLLLISAVQMLNAQPAESRKEDVSEG
                     SSKVDAGASIAVVPLTIPLLTGPATISTMVIYADKTRHWWQLAVLVGYGVVVALATYA
                     VFSASGRIARALGQTGIDVMTRLMGLVLAALAVELLADGMQKLFPVLASVGP"
     gene            1345..3066
                     /locus_tag="PKV56_12385"
     CDS             1345..3066
                     /locus_tag="PKV56_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68656.1"
                     /translation="MSDPSRQALYDYIICGAGTAGCLLANRLSADPTKRVLLIEAGGN
                     DDYAWIHIPVGYLRCIGNPRTDWMYETDAEPGLNGRSLRYPRGKVLGGCSSINGMIYV
                     RGQARDYDRWGAGDGFGPNPGWSWDDCLPHFLKHEDSHRGSDAFHAAPGFDPSGERQG
                     GEWRVERQRLHWPILDAIARAAQQAGLPATDDFNRGCNEGVGYFDVNQRKGVRWNATK
                     GFLRPIEHRENLQVWTGAHVSRLLFESGQNNGSWDGQPRAVGVEVLPRGDRGDDNRGA
                     PLEVRAKVEVILCAGSIGTPQLLQLSGIGAPQALGSLGIPVVLALPGVGENLQDHLQL
                     RSVYAVERTHTLNSRSRSFWHKAQMAIEYALRRTGPLSMAPSQLGAFTRSDPGQPHPN
                     LEYHLQPLSLEAFGEPLHDFDAFTASVCHLNPGSRGWVRLRSADSTAAPVIQPNYLST
                     DEDRRVAVEALRITRRIVEQQALSVYRPREVKPGMHHQTAEELIHQAGDIGTTIFHPV
                     GTSKMGPASDPHAVVDARLRVHGVTGLRIADASVMPTITSGNTGAPTLMIAERAAQWI
                     AEQARGA"
     gene            complement(3079..3648)
                     /locus_tag="PKV56_12390"
     CDS             complement(3079..3648)
                     /locus_tag="PKV56_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX68657.1"
                     /translation="MQTSLAPAAASIAVASQTTIGVVWTDLMAHRSHLVRFAQRKLHD
                     PMLAEDVVHDVFEAVLSGRALFAGRSALRSWLTGILKHKIVDLVRQRAGYDSLDETDS
                     VSDEGESQTHATMAFECQQPRPDELAEQREFLLQTLARIDALPVGLRDVMHLRVLQDQ
                     PTDIVCRSLDISEDNLFVRLHRARKQLLS"
     gene            3880..4602
                     /locus_tag="PKV56_12395"
     CDS             3880..4602
                     /locus_tag="PKV56_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX68658.1"
                     /translation="MPAHILVAEDQIDIRDLLVMNLQQAGYEVTAVGDGRSALDSQNE
                     QSSDLLILDLMMPGLDGLEVCKALRARGRSSPILMLTAKSTELDRVLGLELGADDYLT
                     KPFSLAELLARVKALLRRADLLRAAQSASGASGLSTSTALRNGDLEILPVKRVVRVRG
                     VPLDFTALEFDLLLHFAQHPGHVFSRGQLLNAVWGYTHDGYEHTVTTHINRLRAKLEA
                     DPMRPQLILTVRGAGYKMREAP"
     gene            4646..6202
                     /locus_tag="PKV56_12400"
     CDS             4646..6202
                     /locus_tag="PKV56_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX68659.1"
                     /translation="MSVRFKIALTIFITGALTALGVIATVLFAFERFEHETTYHRANA
                     FLKRVGTTYNGMLDMQQRHPEEFAVFLRSLLFFEPDSQLYLLRADGSVIVSTSAVSLP
                     SGYKVALKPVLEAMGPTPMPYVMGDDPERPGADGGAIIAALSLTSAVIRPDQPVSGYL
                     YLVCQKPIASEGRIEAFRSTFARPALASILAVVALATLLTAWITATVTRPLRRLTEAV
                     GTVQRDGLTAGAAIDAALVRAPDSFEFNSPRDEFGQLASGFRAMLSTLRTQWEALRRL
                     DHFRREGVSNLSHDLRSPLTATTACLETLEARWHADPDRGADRQLVEVALRNTRNAAR
                     LVRSLGDLAQLDEPEFKMHVEVLDAAELLDDIALRFAARADSQGIKLVAEPPGPDDLP
                     TFAALDIELFERAVANLIDNALKFCPVGGHITLAARARRLQDSGSVGHRVEVSVTDTG
                     PGIPAADIACLFDRFYQSRQSAEPASSEGGKGLGLAIVKRIAELHGGDVSVVSAPSVG
                     TTVTLSLPSA"
     gene            6519..7286
                     /locus_tag="PKV56_12405"
     CDS             6519..7286
                     /locus_tag="PKV56_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOX68660.1"
                     /translation="MFDTAVLALASLFAGFVDAIVGGGGLVLVPALFGIFPGAAPATL
                     FGTNKGAAIWGTAWATGQYARKVHLPWDALLPAAATALAGSFLGAWAVTLIEPTGLRR
                     ALPFVLLTVLVYTLVRKDLGREHSPQHHGRTQALLASAVGGVIGFYDGFFGPGTGSFF
                     VFLFVRMLGFDFLHASASAKLLNTATNMGALILFASTGHVWWHLAAVMAVANVLGSLL
                     GTRLALRHGSGFVRGVFICVVTVLIAKTAWDGYSGAG"
     gene            7401..8219
                     /locus_tag="PKV56_12410"
     CDS             7401..8219
                     /locus_tag="PKV56_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX68661.1"
                     /translation="MPVDPELRALNIDIPAQPESLVKLSILLAEEEVNLQHVSALIES
                     DMALASAVLKAVNSSLYGLSGRVQSVHQAITYLGTREVAAVTFEMGLRAVFPGAPELA
                     PVWERAAVRGLLMGRIGYAIGVDPWAAHSAGLFEECGKAVLFRHATSRYKPLMTNAAN
                     DEELVFLEHQEFGVSHDALGAALCESWGLAPAAVHSVRFHVVVNSTRELPVQLQRRSI
                     CVLSAVAHALMTDPETLEDVVNAVAPQAQLEPELVLRGARRVQAQIENAVESAA"
     gene            8285..8557
                     /locus_tag="PKV56_12415"
     CDS             8285..8557
                     /locus_tag="PKV56_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68662.1"
                     /translation="MGASDFLSGGVPRFGARVLADSTQVRRTNEREPAGRRPPVLQSS
                     RGPIQLSLFDHLEPVTQPLQVPEGDAATAIDARAVASPDDSDRSGD"
     gene            complement(8577..8888)
                     /gene="soxZ"
                     /locus_tag="PKV56_12420"
     CDS             complement(8577..8888)
                     /gene="soxZ"
                     /locus_tag="PKV56_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HOX68663.1"
                     /translation="MGQPSRIRATAQGIGAVVRVLMTHEMESGQRRDATGNAVPAWHI
                     TELTASLEGKPVLTAQWGPGVSANPFLQFRLKNAKAGDRVGIAWKDNRGDSRSDEAVV
                     G"
     gene            complement(8900..9388)
                     /gene="soxY"
                     /locus_tag="PKV56_12425"
     CDS             complement(8900..9388)
                     /gene="soxY"
                     /locus_tag="PKV56_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HOX68664.1"
                     /translation="MEPGSGKTRRAALQQSLALAGWLAGAGLLPNTAVAQAAASEWNA
                     AAFGAHTLETVLLALGATRPTLSDEVVLTIPDLADNGAVVPVGISTSLPGVRRIVLLV
                     EKNPTMLVAVFEISDAIDADVALRIKMAESSNVYAAAMLADGRVLYAVKDVQVTLGSC
                     GT"
     gene            complement(9399..11159)
                     /locus_tag="PKV56_12430"
     CDS             complement(9399..11159)
                     /locus_tag="PKV56_12430"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOX68665.1"
                     /translation="MTATAPVLLSLRDLSVSFRIGRQGGAVLRSQAVGRLVDGKAQGI
                     SFDVPESTTVALVGESGSGKSVTAMSILNLLPDNAERQGAIHFQGRDLLTATRHELQA
                     LRGKDIACVFQDPMSSLNPVFTVGAQLCEPLIKHLGLTSRQAMARAEALLAEVGLPEP
                     KRRLGAYPHELSGGQQQRVMIAMALSCEPKLLIADEPTTALDVTIQRQIMELLAGVKA
                     QRRMSMLFISHDLGVVGEIADRVVVMRQGTVREQGPVADIFASPQDAYTRALLACRPT
                     LARTGPRLMVIDEHIAAQDGAGPASLATQAAAVKDPEARIVLEARALTKSFWIPRGLF
                     GRREFRAVGREGRGVTFQLRRGHTLGVVGESGSGKTTMGLALLRLHEPSGGRVGGEVH
                     FDGQDLLALTKGELLPMRRRLQVVFQNPYASLNPRFTIGQTLVEPMLIHGIGSGQDDR
                     ESRARALLRKVGLDETALARYPHEFSGGQRQRVAIARCLTLSPEVLVLDEAVSALDVS
                     VQAQVLNLLKDLQDELGLAYVFISHDLAVVRFMADEVLVMKDGEVVEQASVEQILDAP
                     RQDYTKRLIGAIPRGYQPAA"
     gene            complement(11156..12208)
                     /locus_tag="PKV56_12435"
     CDS             complement(11156..12208)
                     /locus_tag="PKV56_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68666.1"
                     /translation="MSAALPSSAMLRAADRSEGVWLSAWRRFKSDRVGLVSLAIVIAF
                     LLLIALSALGLVAGNWQREVGVPYANPTFLGPTPPPPAAQIEEGKGPPVDISTVDPLA
                     PRYAEWAERASKLKVDEEVRDNMLPLGGDRLGRDVLAKAIKGAEISIFVGVLAALLAT
                     AIGTALGAVSGFLGGRVGDLLEWVYNVFTSIPGILLIFAFAAVFGRGTGTVVLILGLT
                     GWTGIYRLVRAEFLKHAVRDYVRAAEAIGASVGSRMFRHILPNVSHVVLVQLSILTVG
                     FIKAEVILSYLGLGVSIDQVSWGTMLAESQVELLLGHWWQLATATVFMAVFVTAFSLF
                     TDALRDALDPKLRGLE"
     gene            complement(12551..13489)
                     /locus_tag="PKV56_12440"
     CDS             complement(12551..13489)
                     /locus_tag="PKV56_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68667.1"
                     /translation="MAAYLLRRLWQTIPTLAGVILLVFVLFKFFGGDPAEILAGQIAK
                     PDQIAAIRQQLGLDQPWWIQLWIFVKQVLTFDWGRSWATNEAVSNLFLTRLPATLTVM
                     VPILVLEVILAIPLALAVAVVRGSLTDRAVMVLTTVALAISFLVYVIVGQWLFGFKLG
                     WFPVQGWSGSLWTNLVTYAPLPVLLAVMVSLAPQTRLYRSFFLDEIDQDYVRTARAKG
                     LTERVILLRHVLRNAMIPILTSIGTGLPGIFVGSFLIEVFFSIPGLGREVLLAVNRSD
                     YPVIQAVTVYLAAITMLINVLTDVLYKLVDPRVVLK"
     gene            13547..>14164
                     /locus_tag="PKV56_12445"
     CDS             13547..>14164
                     /locus_tag="PKV56_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX68668.1"
                     /translation="MKSGGVRRVLGEVLGSALMLVVLCAGWVVAPSASAQGAAPAQKV
                     FRYAFPVAETGFDPVQISDLYSATIIANIIESPLTYDYLARPVKLKLQTAAEMPEVSA
                     DFKTYTVRIRPGIYFADDPAFKGKKRELVAADYVFSLKRIFDPRWKSPAYPSLADEQV
                     LGLEALRKKSIEERAPFDYDTEIEGLRALDRYTYRITLGKPSPRFI"
BASE COUNT         2362 a         4728 c         4579 g         2495 t
ORIGIN      
        1 gcgggcacag cgcaccgcca ccgccaccgc cgccgccgcc gccaccgccg ccgccgccac
       61 cgccgccgcc gccgccaccg gagcggcctg ctccgccgcc aaatccgccg ccgccgccgc
      121 caccgccacc gccaccgccg aagccaccgc ggcttccgcc accaccaaag ccgcctggac
      181 gctcttcgcg tgggcgtgcc tcattgacaa cgagtgcgcg accgtccatc tgttggccgt
      241 tcattccgtt gatcgcagac tgggcttctg cgtccgaacc catttccacg aagccgaagc
      301 ccttggaacg accggtttcg cgatccatca tgactttcgc ggatgacacg gtgccgaact
      361 gagcaaatgt ttgttgaaga tcgtcgtctc gtacgttgta cgaaaggtta ccgacgtaga
      421 gtttgttgcc cattgagagc gcctttcttt ccagaaaaca cagaatatct gtgtagcttc
      481 aacctatcct gacccattca agcaaaggcg ctgctactga acacagaatt ttcattatca
      541 attaactgat cgattttcgg caaaggcttc tcaaatcgct tgtcaagaaa gtgttgtttt
      601 gtagataccc cggcagcgtt ctacctgtga gagattgaaa agccacgaaa gttagtgggc
      661 gctaatgtct ggcgctgcgc cccacaatcg accatggaca tcctcaaacc cctgatcgcg
      721 ttgctggcgg tcgtcaatcc gatcggggtc gtgccgttct ttctccattt cacgcagaat
      781 ttcacgcccg agcagcgtcg ccgcacgatc cgggtcagcg cggtcagcgc ctttgtggtg
      841 atcgccgtca gtgctttggc agggttgcag atcatccagt tcttcgggat atcactggcc
      901 tcgttccaag tcggcggcgg attgcttctg ctgatctccg ctgtccagat gctgaacgcc
      961 cagcccgccg aatcccgaaa ggaggacgtg agcgaaggca gcagcaaggt cgacgcaggc
     1021 gccagtattg cggtggtgcc gttgaccatt ccgctgctga ccgggcctgc caccatctcc
     1081 acgatggtga tctatgccga caagacccgg cactggtggc aactcgcggt gctggtcggt
     1141 tatggcgttg ttgtcgcgct cgcgacgtac gccgtcttca gtgcctcggg gcgcattgca
     1201 cgtgcgctgg gacagaccgg catcgacgtc atgacccgac tgatggggct ggttctcgcg
     1261 gcactggctg tcgaattgct ggccgacggc atgcagaagc tgtttccggt tctggccagc
     1321 gtcggtccct gacggatctg ccccatgagt gatccgtccc gacaagccct ttacgactac
     1381 atcatctgtg gcgcgggtac tgctggttgc ctgctggcga atcggctgag tgccgacccg
     1441 acgaagcggg tgcttctgat cgaggccggg ggcaacgacg actatgcgtg gatccacatt
     1501 ccggttgggt acctgcgctg catcggtaac ccgcgcaccg actggatgta tgaaaccgac
     1561 gccgagcccg gcttgaacgg ccgctcgctg cgctatccgc gtggcaaggt gcttggcggc
     1621 tgcagcagca tcaacgggat gatctatgtg cgcggccagg cccgtgacta cgaccgctgg
     1681 ggcgcgggcg acggattcgg tccgaatcct ggatggagct gggacgactg cctcccgcac
     1741 ttcctgaagc acgaggattc ccaccgtggt tccgatgcct ttcatgccgc ccccggtttc
     1801 gaccccagcg gggagcgcca gggtggtgag tggcgagtcg agcggcaacg cctgcactgg
     1861 ccgatcctcg acgcgatcgc acgggcggct cagcaagccg ggctgccggc caccgacgac
     1921 ttcaaccggg gatgcaacga aggagtcggc tacttcgatg tgaaccagcg caaaggcgtg
     1981 cgctggaacg ccaccaaggg attcctgcgg ccgatcgagc atcgggagaa cttgcaggtc
     2041 tggaccggcg cgcatgtctc gcgcctgctg ttcgaatccg gccagaacaa tggatcgtgg
     2101 gacggccaac ctcgcgcggt cggtgtcgaa gtgcttccac gcggcgaccg gggggacgac
     2161 aaccggggtg cacccttgga agtgcgtgcg aaggtcgagg tgatcctttg cgctggctcc
     2221 atcggtacgc cacagttgct gcagctatcg ggcatcggag ctccgcaggc gctgggttcg
     2281 ctgggcattc cggtcgtgct tgccttgccg ggtgtcggcg agaacctgca ggatcacctg
     2341 caactgcgct cggtttacgc ggtcgagcgc acgcacaccc tgaacagccg gtcgcggtcg
     2401 ttttggcaca aggcccagat ggcgatcgag tacgcattgc ggcgaaccgg gccgctgagc
     2461 atggcgccgt cgcagctcgg tgccttcacg cgcagcgatc ccggtcagcc gcacccgaat
     2521 ctcgagtacc acctccagcc gctgtcgctc gaggccttcg gcgagccgct gcacgacttc
     2581 gatgccttca ccgccagcgt ttgccacctg aatcccggga gtcgtggctg ggtgaggctg
     2641 cgaagcgccg attcgaccgc ggcacctgtc attcagccga actacctgag taccgatgaa
     2701 gaccggcgcg tggcagtcga agcgcttcgc atcacaagac gcatcgtcga gcagcaggcc
     2761 ttgtcggtct accggccgcg agaggtgaag cccggcatgc atcaccagac cgccgaggaa
     2821 ctgattcatc aggcaggtga catcggcacg acaatcttcc atccggtcgg gacgtcgaag
     2881 atgggcccgg cgagcgatcc gcatgccgtg gtcgatgccc gcctgcgcgt gcacggggtg
     2941 accggcttgc gtatcgccga tgcgagcgtg atgccgacca tcaccagcgg caacaccggc
     3001 gcaccgacac tgatgatcgc ggagcgtgcg gcgcagtgga tcgccgagca ggcgcgtggc
     3061 gcctgacggc gccgctgttc aggaaagcag ctgcttgcgg gcgcggtgca accgcacgaa
     3121 gaggttgtcc tcgctgatat cgaggctgcg gcagacgatg tcggtgggct gatcctgcag
     3181 cacccgcagg tgcatcacgt cgcgcaggcc gactggcaac gcatcgattc gggccagcgt
     3241 ctggagcagg aattcgcgct gttcggccag ttcgtctgga cgcggttgct ggcactcgaa
     3301 tgccatcgtt gcgtgtgtct ggctttcgcc ctcgtcactc acgctgtcgg tctcgtccag
     3361 gctgtcgtag ccggcgcgct gacggaccag atcgacgatc ttgtgcttca ggatgccggt
     3421 cagccacgag cgcagtgccg agcggccggc aaacaaggcg cggcccgaca acaccgcctc
     3481 gaacacatcg tgcaccacgt cctcggccag catcgggtcg tgcagcttgc gctgggcgaa
     3541 gcgcaccagg tgactgcggt gggccatcag gtcggtccag accaccccga tcgttgtctg
     3601 cgaggcgacc gcgatggacg cggctgcggg ggcgagggag gtctgcatgg tgggctccgg
     3661 atccgttgag tcgatgcagc gactgtggca accgcacctc gcgccggcct cgccaaacct
     3721 cacagccacc taacatttgg atcaccggtg ccccgcctct tcgcgcccct gttccgtggt
     3781 ggctggtgaa atgcacgggt ggcgtccgga ctgggcccgt cttcgaaccg ctggtccccg
     3841 tgccgggtaa gaaccagagt cgcggcgcga caattcacca tgcccgccca catcctcgtc
     3901 gccgaagacc agatcgacat ccgtgacctg ctggtgatga acctgcagca ggcgggatac
     3961 gaggtcaccg ctgtcggcga tggccggtcc gcgctggaca gccagaacga gcagtcgagc
     4021 gacttgctga tcctcgatct gatgatgccg gggctggacg ggctggaggt ctgcaaggcg
     4081 ctgcgcgcgc gcggtcgaag ctcaccgatc ctgatgctga ccgccaagtc gaccgagctt
     4141 gatcgcgtgc tggggctgga gctcggtgcc gatgattacc tgaccaagcc gttctcgctg
     4201 gccgaactgc tggctcgggt gaaggcgctg ctgcgaaggg cggatctgtt gcgtgcggcg
     4261 cagagcgcct ctggcgcgtc cggactgagc acgagcacgg cgctgcgcaa cggggatctc
     4321 gagatcctgc cggtcaagcg ggtggtgcgg gtgcgcggtg tgccgctgga tttcaccgcg
     4381 ctggagtttg atctgctgct gcacttcgcc cagcacccgg gccatgtgtt ctcgcgcggc
     4441 cagctgctga atgcggtctg gggctacacc cacgacggct acgagcacac ggtgaccacg
     4501 cacatcaatc gactgcgcgc caagctcgag gccgatccca tgcgtccgca actgatcctg
     4561 accgtgcgcg gggccggcta caagatgcgc gaggcgccgt gatgactcgg cttgtttcgc
     4621 gaagctattc gggccggcca acggcatgag cgttcgcttc aagatcgcgc tgacgatctt
     4681 catcaccggt gcgctgacgg ccctgggcgt gatcgccacc gtgctgtttg ccttcgagcg
     4741 tttcgagcac gagaccacct accaccgtgc caatgcgttc ctcaagcgcg tgggcaccac
     4801 ctacaacggc atgctcgaca tgcagcagcg acacccggaa gagtttgccg tcttcctgcg
     4861 cagccttctg ttcttcgagc ccgacagcca gttgtatctg ctgcgtgccg acggttcggt
     4921 gatcgtctcg accagcgcgg tctcgttgcc atcgggctac aaggtggcgc tgaagccggt
     4981 gctcgaggcg atgggtccga cgccgatgcc ctatgtgatg ggagatgacc ccgagcggcc
     5041 gggcgccgac ggtggcgcga tcatcgccgc gctgtcgctg accagtgcgg tcatccgccc
     5101 tgatcaaccc gtttctggat atctgtacct ggtctgccag aagccgatcg cgtccgaggg
     5161 ccggatcgag gcgttccgca gcacctttgc ccgacccgcg ctggcttcga tcctcgcggt
     5221 cgtggccttg gcgaccttgc tcaccgcctg gatcacggcc accgtgacgc ggccactgcg
     5281 gcggcttacc gaagcggtcg gcaccgtgca gcgcgacggg ctgaccgccg gggctgccat
     5341 cgacgcggcg ctggtccgcg caccggacag cttcgagttc aattcgccgc gggacgagtt
     5401 tggtcagctg gcgagtggtt tccgggcgat gctgtcgacg ctgcgcacgc agtgggaggc
     5461 cctgcgtcgc ctcgatcact ttcggcgaga gggcgtcagc aacctgtcgc acgacctgcg
     5521 gtcgccgctg actgcgacga ccgcctgcct cgaaacgctg gaggcgcgct ggcatgccga
     5581 cccggatcgg ggcgccgatc gccaattggt cgaggtggcc ctgcgcaaca cccgcaatgc
     5641 ggcccggctg gtccgctcgc tgggcgatct tgcgcagctc gacgagcccg aattcaagat
     5701 gcatgtcgag gtgctcgacg cggccgaact gctcgacgac atcgcgctgc gctttgcggc
     5761 gcgggccgac agccagggca tcaagctggt agccgaaccg cccggacccg atgacctgcc
     5821 cacttttgcc gcactcgaca tcgaactctt cgagcgcgcg gtggccaacc tgatcgacaa
     5881 cgcactgaag ttctgcccgg tcggcggtca catcacgctg gcagctcgcg cgcggcgctt
     5941 gcaagacagt ggatccgtcg gacatcgggt cgaggtcagc gtcaccgaca ccggtcccgg
     6001 cattcctgcc gctgacatcg cttgcctgtt cgatcgcttc taccagagtc gacagagcgc
     6061 ggagccggcc tcatccgaag ggggcaaggg gctgggtctg gccatcgtca agcgcattgc
     6121 cgaactccac ggtggtgacg tctcggtggt cagcgccccg tcggtgggca ccacggtcac
     6181 gttgtcgctt ccttccgcct gagccttata aaacgacgtt cgcaagccgg ttttctgcga
     6241 ccgtgacggc cacgcaacat ggtcgggaaa ccccgtatgc ccccctttgg ggtgcacccc
     6301 tagagtccgt tttctcgcga aggcaccttc taggtggctt tctgatgcgg gggtgatgag
     6361 gagacataaa tagcgaaggg ggcgtattgc ctcactgacg catcgacagt gccgcaccat
     6421 cggcccatgt cagaatcgaa aggcgccggg agtcccggcg ccttttcgtt tttccgggcg
     6481 gcacgcgccc tttttcatcc cgggcctggc tccgtccgat gttcgacacc gccgtcctgg
     6541 ctctggcctc gctgttcgca gggtttgtcg atgcgatcgt cggtggaggc ggcctggtgc
     6601 tcgtcccggc gctgttcggc atcttcccgg gggcggctcc tgccaccctt ttcggcacca
     6661 acaagggcgc ggcgatctgg ggtacggcct gggccaccgg acagtacgcg cgcaaggtgc
     6721 atctgccctg ggacgcactg ttgccagccg ctgccaccgc tctggcgggc agtttcctgg
     6781 gcgcctgggc agtcaccctg atcgagccga ccggcctgcg tcgtgccctg ccgttcgtgc
     6841 tgctgacagt gctcgtctac acgctggtgc gcaaggatct cggccgcgag cacagcccgc
     6901 agcaccacgg acgcacccag gcactgctgg ccagtgccgt cggtggggtg atcgggttct
     6961 atgacggatt cttcggacct ggcaccggca gtttcttcgt cttcctgttt gtgcgcatgc
     7021 tgggtttcga cttcctccat gcgagcgcca gcgccaagct gctcaacacc gcaaccaaca
     7081 tgggcgctct gatcctgttt gcgagcacgg gccatgtctg gtggcatttg gcggcagtga
     7141 tggccgtggc caacgtgctt ggcagcctgc tcggtacgcg cctggcgctg cgccacggct
     7201 cgggcttcgt gcgaggtgtg ttcatctgcg tggtgaccgt gctgatcgcg aagaccgcct
     7261 gggatgggta ctccggcgcc gggtgatccg gggcgatccg ctgccgaatt gagcggtccc
     7321 gaccccgttg tttgccgcta tgcgcctgtg gcgactgcct aggatcgggc gaatgtcaaa
     7381 aatctgactg gaagactgcc atgcccgtcg atcccgaact gcgcgcgctg aacatcgata
     7441 tcccggccca gccggagtcg ttggtgaaac tctccatcct gctcgccgaa gaggaggtga
     7501 acctgcagca cgtcagcgcg ttgatcgaat ccgacatggc cctggcgtct gcggtgctga
     7561 aggcggtcaa ctcttcgctc tacgggctgt cgggtcgggt gcagagcgtg caccaggcga
     7621 tcacctacct cggcacacgc gaggtcgctg ccgtcacttt cgagatgggc ctgagggccg
     7681 tcttcccggg tgcgccggaa ctggcgccag tgtgggagcg cgccgcggtg cgcggcctgc
     7741 tgatgggtcg cattggctat gccatcgggg tcgatccgtg ggctgcccac tctgccgggc
     7801 tgttcgagga gtgtggcaag gccgtgctgt tccgtcacgc gacgtcacgc tacaagccac
     7861 tgatgacgaa tgccgcgaac gacgaagaac tggtgttcct cgaacaccag gagttcggcg
     7921 tcagccacga cgcactcggt gcggctctct gcgaaagctg gggacttgcg ccggcggccg
     7981 tgcacagtgt gcgtttccac gtggtcgtca actcgacccg ggagctcccg gtccagttgc
     8041 agcgccgttc gatctgcgtg ttgtcggcgg tcgctcatgc tctgatgacc gatccggaga
     8101 cgctcgagga cgtggtcaac gcggtcgcgc cgcaggccca gcttgagccc gaactggtgc
     8161 tgcgcggggc acgacgggtg caggcgcaga tcgagaacgc tgtcgagagt gccgcgtgaa
     8221 cgacaggcgc gcgatgccgt ctcggtcgcc ccagctccgg cgcgactgat cggcggatca
     8281 ggcgatgggc gcttcggact ttctttcagg cggcgtgccg cgcttcggcg cgcgcgtgct
     8341 ggccgactcg acgcaggtgc gccggacgaa cgagcgcgaa ccggcgggtc gccggccccc
     8401 ggtgctgcaa agcagccgcg gcccgatcca gctgtcgctg ttcgatcacc ttgagcctgt
     8461 cacccaaccc cttcaggtcc cggagggtga tgcggcgacg gccatcgacg cccgggctgt
     8521 tgcttctccg gacgattcgg atcgcagcgg cgactgagac cgcacctgcg cgcagatcag
     8581 ccgaccacgg cttcgtcgct gcggctgtct ccccggttgt ctttccaggc aatgcccacc
     8641 ctgtcgcccg ccttcgcgtt cttcaacctg aactgaagga acgggttggc cgacacgccc
     8701 ggcccccact gtgccgtcag caccggcttc ccctcgagcg acgccgtcag ctcggtgatg
     8761 tgccaggccg gaaccgcatt gcctgtcgca tccctgcgct gcccgctttc catctcgtgc
     8821 gtcatcagca cgcgcaccac ggccccgatg ccctgggcgg tggcgcggat gcggctgggt
     8881 tgtcccatcg acttgcgcgt caggtgccgc agctgcccag cgtcacctgc acatccttga
     8941 cggcgtagag cacccggccg tcggcgagca tcgccgccgc gtaaacattg gagctttcgg
     9001 ccatcttgat gcgcaaggcc acatccgcat cgatcgcatc cgaaatctcg aataccgcca
     9061 ccagcatcgt cgggttcttc tcgaccagca gcacgatgcg ccgcacaccc ggcagcgagg
     9121 tcgagatgcc gaccggcacg actgcaccgt tgtctgccag gtcggggatg gtcagcacca
     9181 cctcgtcgct caatgtcggg cgggttgcac ccagtgcgag cagcaccgtt tcgagggtgt
     9241 gtgcgccgaa cgccgccgcg ttccattcgg aggcagcggc ttgcgcaaca gctgtgttcg
     9301 ggagcagtcc ggccccggcc agccagcccg ccaaagccag gctctgctgg agcgctgcgc
     9361 gacgggtttt gccgctgccc ggttccatgt tcgacgcttc aggccgccgg ctggtagcca
     9421 cgaggaatgg caccgatcag ccgcttcgtg tagtcctgcc gaggcgcgtc cagaatctgc
     9481 tcgacgctgg cttgttcgac cacttcgccg tccttcatca ccagtacctc atcggccatg
     9541 aagcgcacca ccgcgaggtc gtggctgatg aagacatagg ccagcccgag ttcatcctgc
     9601 aggtccttca gcaggttcag cacctgtgcc tgcacgctga catcgagcgc gctgacggcc
     9661 tcgtcgagca ccagcacctc ggggctcagt gtcaggcagc gggcgatggc gacgcgctgg
     9721 cgctgaccac cggaaaactc gtgcgggtag cgagccaggg ctgtttcgtc cagaccgacc
     9781 ttgcgcagca gcgcgcgagc gcgtgactct cggtcgtcct gccccgagcc gataccgtga
     9841 atcagcatcg gctcgaccaa tgtctggccg atcgtgaagc gcgggttcag actggcgtac
     9901 gggttctgga aaaccacctg caggcgccga cgcatcggca gcaattcgcc cttggtcagc
     9961 gcgagcagat cctgtccgtc gaagtgcacc tcgccaccaa cccgaccgcc gctcggctcg
    10021 tgcagcctca gcagcgccag tcccatcgtc gtcttgcccg agccggattc cccgaccacg
    10081 cccagcgtgt ggccgcgccg cagctggaac gtgacgccgc gtccctcccg gccgactgcc
    10141 ctgaactccc tgcggccgaa cagcccacgg ggaatccaga aactcttggt caaagcgcgg
    10201 gcctcgagga cgatcctcgc ctccgggtcc ttcacagccg ccgcctgggt cgccagggac
    10261 gcgggcccgg cgccgtcctg tgcagcgatg tgctcgtcga tcaccatcag ccggggcccg
    10321 gtgcgcgcca gcgtcggtcg gcaggccagc agcgcgcgcg tgtacgcatc ctgcgggctg
    10381 gcgaagatgt cggccacggg gccctgttcg cgcacggttc cctgccgcat cacgaccacg
    10441 cggtcggcga tctcgccgac cacaccgagg tcgtggctga tgaacagcat gctcatgcgc
    10501 cgttgcgcct tcactcccgc gagcagttcc atgatctgcc gctggatcgt cacatcgagg
    10561 gcggtggtcg gttcgtcggc gatcagcagc ttcggctcgc aagacagcgc catcgcgatc
    10621 atcacccgtt gttgctgacc gccggacaac tcgtgcgggt aggcgccgag ccgtcgcttc
    10681 ggttcgggca atcccacttc ggcgagcagc gcttcggcac gcgccatcgc ctgccgcgac
    10741 gtcagaccca gatgcttgat cagtggctcg cacagctgcg caccgacggt gaagaccggg
    10801 ttcagggacg acatcggatc ctggaacacg caggcgatgt ccttgcccct cagcgcttgc
    10861 aactcgtgtc gcgtggcggt cagcaggtcg cggccctgga agtggatcgc gccctgccgt
    10921 tcggcgttgt cggggagcag gttcaggatc gacatcgcgg tgacgctctt gcccgagccc
    10981 gactcgccaa ccagcgcgac ggtggtgctt tccggcacat cgaaactgat gccttgcgcc
    11041 ttgccgtcga ccagtcgacc gactgcctgc gaacgcaaca cggcaccgcc ttgcctgccg
    11101 atgcgaaacg acacgctcag gtcccgcagg ctcagcaaca caggcgcggt ggccgtcatt
    11161 ccagccccct cagcttcgga tcgagcgcgt cgcgcagcgc atcggtgaac agcgagaacg
    11221 cggtgacgaa caccgccatg aacaccgtcg cggtggccaa ctgccaccag tggccgagca
    11281 gcagttcgac ctgcgattcg gccagcatcg tgccccacga gacctggtcg atcgacacgc
    11341 cgaggcccag gtagctgagg atcacctcgg ccttgatgaa acccaccgtc aggatcgaca
    11401 gctgaaccag cacgacgtgg ctgacgttgg gcaggatgtg tcggaacatg cgcgagccga
    11461 cgctggcgcc gatcgcctcg gccgcacgca cgtagtcgcg caccgcgtgc ttcaggaact
    11521 cggcgcgcac cagcctgtag atgccggtcc agccggtcag cccgaggatg agcacgaccg
    11581 tgccggtgcc gcgtccgaac accgctgcga acgcgaagat cagcaggatg ccgggtatcg
    11641 aggtgaacac gttgtagacc cactccagca ggtcgccgac gcgcccgccg agaaagccgc
    11701 tgacggcgcc cagcgcggtg ccgatcgcgg tggccagcag ggccgccagc acgcccacga
    11761 agatcgagat ctcggctccc ttgatcgcct tcgccagcac atcgcggccg agccggtcgc
    11821 cgcccagcgg cagcatgttg tcgcgcacct cctcgtcgac cttcagcttc gacgcgcgct
    11881 ctgcccattc ggcatagcgc ggcgccaagg gatcgactgt ggagatgtcg accggcggtc
    11941 ccttgccttc ctcgatctgc gcggccggag gcggtggcgt cggcccgagg aacgtcgggt
    12001 tcgcataggg cacgccgacc tcgcgctgcc agttcccggc caccagcccg agcgccgaca
    12061 gcgcgatcag cagcaggaac gcgatcacga tcgccagcga taccagcccg acgcggtcgc
    12121 ttttgaaacg acgccacgcc gacagccaga cgccttcgga tctatccgca gcgcgcagca
    12181 tcgcagacga cggcaaggcg gcgctcattt cattggctcc accgcgtgtt gcgcgatcca
    12241 cgacccatga aacgggcatg tgaggtgggc gcgcgtgccg gggacactgg gtacctccct
    12301 ccgcgaatgt cccccggctt cgcctcctcc tttatttcgc tgcggaagat acccagtgcc
    12361 cccggcacga gagacgctgg cagcaggcgg tcgaacaaac acggtgctga ttgatcatgt
    12421 cggcggtgtg cgctgcgcag cgaaataaag aaggaggccg caggccgggt gacattcgcg
    12481 gagcagtgca cgccgtcggc gtgatcgcgc gcatcttcaa cccgcaggca aggaattgag
    12541 gagttcggat tcacttcagc accacccgcg ggtcaaccaa tttgtagagc acatcggtga
    12601 gcacattgat cagcatcgtg atcgcggcca ggtacaccgt gaccgcctgg atcaccgggt
    12661 agtcgctgcg gttgaccgcc agcagcacct cgcgcccgag cccggggatc gagaagaaca
    12721 cttcgatcag gaacgagccg acgaagatgc ccggcagccc ggtgccgatg ctcgtcagga
    12781 tcgggatcat cgcgttgcgc agcacatggc gcagcaggat cacgcgctcg gtcagtcctt
    12841 tggcgcgcgc ggtgcgcacg tagtcctggt cgatctcgtc gaggaagaaa ctgcggtaca
    12901 gccgcgtctg cggcgccagg ctgaccatca ccgccagcag caccggcagc ggcgcgtagg
    12961 tgaccaggtt ggtccacagc gagccgctcc agccctgcac cggaaaccag cccagcttga
    13021 agccgaacaa ccactggccg acgatcacgt agaccaggaa cgagatggcc agggcgaccg
    13081 tcgtgagcac catgaccgcg cgatcggtga gcgagccgcg caccacggcg acggccagcg
    13141 ccagagggat cgcgaggatc acttcgagca ccaggatcgg caccatcacc gtgagcgtgg
    13201 ccggcagccg agtcaggaac aggttggaca ccgcctcgtt ggtcgcccag ctgcgacccc
    13261 agtcgaaggt cagcacctgc ttgacgaaga tccacagctg gatccaccac ggctggtcga
    13321 ggccgagctg ctggcggatc gcggcgatct ggtcaggctt ggcgatctgc ccggccagga
    13381 tttccgccgg gtcaccgccg aaaaacttga acaacacgaa caccagcagg atcacaccgg
    13441 ccagcgtcgg gatggtttgc cagagtcgtc tgagcaggta ggcggccatg gggtggagag
    13501 tctagactgc gcccgctcgg cgggtcgtct gccaggagaa gcgcgaatga agtcaggtgg
    13561 tgtgcgtcga gttctgggcg aggtgcttgg ttccgccctg atgctggtgg tgctgtgcgc
    13621 cgggtgggtg gtcgccccca gcgcttcggc gcagggggcg gcgccggccc agaaggtgtt
    13681 tcgctacgcc ttccccgtcg ccgagaccgg cttcgacccg gtgcagatct ccgacctgta
    13741 ctcggcgacc atcatcgcga acatcatcga gtcgccgctg acctacgact acctggctcg
    13801 accggtcaag ctgaagctgc agaccgccgc cgagatgccg gaggtgtcgg ccgatttcaa
    13861 gacctacacg gtcaggatcc ggcccggcat ctactttgcc gacgacccgg ccttcaaggg
    13921 gaagaagcgt gaactggtcg ccgccgacta tgtcttctcg ctgaagcgga tcttcgatcc
    13981 gcgctggaag agccccgctt acccgagcct cgccgacgag caggtactgg gcctcgaagc
    14041 cctgcgcaag aagtcgatcg aggagcgagc gcccttcgac tacgacaccg agatcgaggg
    14101 actgcgcgcg cttgatcgct acacgtaccg catcacgctg ggcaaaccca gcccgcgctt
    14161 catc
//
LOCUS       DAOORV010000093        14115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5278_length_14115_cov_8.535064, whole genome shotgun sequence.
ACCESSION   DAOORV010000093 DAOORV010000000
VERSION     DAOORV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_14115_cov_8.535064"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKV56_12450"
     CDS             complement(<1..927)
                     /locus_tag="PKV56_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68669.1"
                     /translation="MPEIHFPTLLFYCQHSLGIGHLTRSFALAAALTKQFRVVFLNGG
                     RLPPGVQVPAGIELIDLPPLGMDDGHTVVSRDRSLDVVAARTERRRLIDAAVVSARPD
                     VLLVELFPFGRKKFANEILPMIRAAKRQSKGPALVACSLRDILVDARPDQKHHDDRAR
                     WLTDRYFDTVIVHADAGFARLEDTFRPSRAMRTPVFHSGYVVPTRQGGSDLKRQERLL
                     ISAGGGIVGQQLFHTVLEARARMRLGLAMRVIAGPFLPEEHWQDLQRQGAGQAGVELV
                     RHVSDMVAEMRSARASISQCGYNTALDIVMAGV"
     gene            complement(920..2713)
                     /locus_tag="PKV56_12455"
     CDS             complement(920..2713)
                     /locus_tag="PKV56_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68670.1"
                     /translation="MTEQASLPGARLRSIVRASLREQRVALGLAAFGLCGVVLAEVLA
                     PWPLKVIFDHILLAKPLPSALSWLQPLLDWGTWPALLSAAGAIAAIALASGALSYLQL
                     YTSARVGHRITWRLRGELFTHLQRLSLAHHRAQRTGELITKVAGDTNLLRDMFSEWAL
                     TLGRHAVTLVAMLAVMFMLNWQLALVAAVTLPPLLGVIYVLNRRVKDSVRQQRRHEGR
                     MASRLNEVLSSIALVQAFGRQGFEEDRFRSEIEANYESGMRSTRASGAIVKAIAVISA
                     AGTAITILMGAREVLAGRLTPGELLVFITYVTSLYKPVRDLARLAGKFSRAGVSAQRV
                     SEILDLEPDRPDAADAMDLRSPAGEIVFEDISFGYAAERPVLDRLNLRIAAGERVALV
                     GPSGAGKSTLMNLLLRLYEPSAGRILIDGIDIRRYSRESLRREIGIVLQDNLLFGVSV
                     RENIAYGRPDATPEAIESAARAARAHEFIVDLPQGYDTELGERAASLSGGQRQRLCLA
                     RALVKEPAILVMDEPTASIDATSARLIHESVARVHRGRTLVVIAHDLTDMSSYDRVLV
                     LQDGRLVEQGTHESLMRSRGTYLALVERRHA"
     gene            complement(2710..3987)
                     /locus_tag="PKV56_12460"
     CDS             complement(2710..3987)
                     /locus_tag="PKV56_12460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOX68671.1"
                     /translation="MTEALAVDQRIETCLDARAMQAVLQKTLPACASGALSIDSISIS
                     KVRRSSSRQRNPNPLTLCYELSLRDLASGRTSTETYYGKVYRDGTASDVANDIQVQCV
                     PSLDLLLWRWPADPGLPQLPALLDLQQTCPWWGKAAEQVVALRHVPEDRATLRYSRRT
                     SKGEEHLYAKTFCDGRASAVHERFRYFWTMSQLGAQFPVVAQPLAHHPETHTFWQSQA
                     PGVPLVQIVGGSGEAGLPDRVAHALSLMHAAPLSLAGPLPRDRVHALTEVRRRQKKIS
                     RTVPELELRVARLADALEMASRQLPEPPIGLIHGDCHPEQFWVAHGQVVLFDFDEFAL
                     GDPMEDLAAFVTKVSLLSGGPAFATAMLAAYSRHAPTRFDGRRLHWHLAVQQLLQAGR
                     AFVFQVPDWRSHIESRLSRAESLADQLLLECSP"
     gene            complement(3984..5363)
                     /locus_tag="PKV56_12465"
     CDS             complement(3984..5363)
                     /locus_tag="PKV56_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOX68672.1"
                     /translation="MSTNASLPTDPLLPNLAVVVDPRRAAEVFGELLHKQGLVVEGCI
                     VDRVKYRPRRNCTLSYRLRLRDRHDGRAFEQRVAARLCGDDAIQRARRAAAEPVAPLV
                     ASRAGPALQSIPRLGLLTWWWPNDPKLVATHVLSDVERLHGEVLPQVVSALSGGRGRL
                     VGHSIEIAQYVPEQRLTARVDLQWQCGAQAPESVRLYAKSTREPDGETCHAILRELQH
                     SAACRDGRLRTPKAVLWQPGAGFHWQEGLAGVSLRDVPPQRTFISARQLGAQVAALHA
                     TPVSLARELTPSMLHQRLADVLAVLTDALPDSQTGLQHLAARLAAGLQALPFDRPATL
                     HGDLHPLNILVDGCGPALIDLDNVVRGPAELELGAWVAEGLYRALLDDADPLRDQSAW
                     QALLDGYADAGGSRFELPGLGWAIAWNLLTQRVWRCVVNLKPGRFERVGALIALASDL
                     AFQRQAEVV"
     gene            complement(5360..7447)
                     /locus_tag="PKV56_12470"
     CDS             complement(5360..7447)
                     /locus_tag="PKV56_12470"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOX68673.1"
                     /translation="MSALFPAAHRLAAEPESDALFLPEWCGLDDAALLAHYRSDRGVV
                     FDAIVDPEEVLPKRLEGLMHGRFEFNGETHSLGDPIDWLQNPSADIEWHILLHKFYYA
                     AGLSQAWQRSADLRYVRRWAELIDGWMRVTPPGFIAADVTGRRVQNWIYSLQGLVLPG
                     MDGREAPVDPAFFRRLLQSIHAQVEFLCANLTPKRNHRTLELLAVFLAGVVFPEFARA
                     QHWRSFALRETLVNIHADLLPDGVHCELSTDYHHLALRNWFQVRTLAARNGHGVPAAM
                     DEALLRAFDFSVHVHQPDGSAPSFSDGDARGYRALLRQAAHLYRRPDLLFVATGGADG
                     VAPLQRNAHFSNSGYHVLRSEWPANGSAEAQHLMFDCGPLGEGNHGHFDALSFELFAF
                     GRPLVVDPGRYTYSEAGETNWRVHFRSTAAHSTVCVDGRSQTRYSPRAIKEPSRHAQG
                     AVRHKISGPPPDTLLIETHDGGSLDLLHGRCSSHEYDAVHDRCIAFIDRRYWIVSDWL
                     RAPGEHDYVVSFQLGTAAASASSLHRAAGLRLDSPHLSIALPARPDQRTELAGAWVSA
                     RYGHKDAAPVLRTHARGRDADFDAVLVPWGCEAPDLQVEDRVVRSGEGLAGHALRIRL
                     AQDGQTLHDGWFHDRGVHSETWLIGTHVFKGRWIHWRETADGRIVRALSHAGATLRDL
                     NGQAVAVEIGEAP"
     gene            complement(7444..8802)
                     /locus_tag="PKV56_12475"
     CDS             complement(7444..8802)
                     /locus_tag="PKV56_12475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68674.1"
                     /translation="MNQPSVAYVMNGFPRLSETFITHEIHQLERLGLPLRLYSVKREN
                     ESVVHPVVEAIRAPLTYLPRASSLSGTSLPGWLKQNLPAFWRSHARVVVRHPVRWAGA
                     LGSALALAWQHRSRDESGRLRLRKVFIKEFLQAGEIAADVLRQGQVGHLHGHFCHGVA
                     TITWLAARMCGLGYSFTAHAKDIYQAELNPGDLLERKMHGARFVATCTCANAQVLRER
                     HPRPEEVHAIYHGLDTEWFAPTGRAAGSGNTRPLILSVGRLVEKKGFDQLIEACARLR
                     DASVDFDCLIVGEEGNASSTVRSLIQQLRLEDRVKLHGAVTQAGLRDIYARAHAFALP
                     CQIMEDGDRDGFPNVLAEAMAMGIPVVSTAISGIPEMIDDGVHGLLVPPRDPVALSQA
                     LGRLLTDAALHQALSMSARARICDRFDSRRTTVLLRDLFMKQLRSPTSDARTTVAAKV
                     TP"
     gene            complement(8810..10060)
                     /locus_tag="PKV56_12480"
     CDS             complement(8810..10060)
                     /locus_tag="PKV56_12480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68675.1"
                     /translation="MSASPHDQPGSIGLLVKIFPKLSETFVLEEVLGLERLGVPIRLY
                     TLAAPTDAVAHPVVERVRAQVVQVPAATWRHAPALLWRHCVLAATSPWRYAKSLRHAL
                     SRQRQGLRDFVRAGWLVGQLRQDGVAHLHTHFISTPADVSELVSTLGGIPFSISAHAK
                     DIYLSNPADLQRKMTAARFTVTCTDYNCRTLSAMAPGANVHRMYHGIDAGVFNPLHRQ
                     TPALVPLILSVGRLRQKKGLDTLIKACMLLTSRGVPYRCEIVGYGEEKAPLSALIEDL
                     ALADQVQLVGKLTREQVIDRYARAAVYVQPSRVAADGDRDGIPNVLLEAMAMGVAVVA
                     TRVSGIPEVVRHRDNGWLVEPDDAAALADAIEKLVEAPELRAALAREARSTVTTHFDN
                     DRNLRLVIQLLAGGHAHPASCVAA"
     gene            complement(10057..10617)
                     /locus_tag="PKV56_12485"
     CDS             complement(10057..10617)
                     /locus_tag="PKV56_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOX68676.1"
                     /translation="MKATRASTNYAPLTRHLHWVMLAVLIGVFSCIELRVLFAKGSDP
                     REALKGGHALLGLTVLALTTLRLFARFGQTTPPIQPAPPRWQQHAATAMHALLYAWLL
                     VMPLAGWLMLSAAGKPIPFFGLELMPLVAPDKELASLVKECHEIAGRCGYVLIGFHAA
                     SALAHHFIRRDDTLLRMLPLRTRAGS"
     gene            complement(10617..11906)
                     /locus_tag="PKV56_12490"
     CDS             complement(10617..11906)
                     /locus_tag="PKV56_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX68677.1"
                     /translation="MKRVLIYSHDTFGLGNIRRMLEVARHLVQSSPEVSVLVITGSPM
                     LHAFRIPEGIDYIKLPCLSRGVDGKYGARSLPMTLEATVRLRANLIRSAITDFAPDLI
                     LVDKKPFGVEDELAGALEGLPSAEVRPKLVLLLRDILDSAESTTRIWRKNGYFEAITA
                     YYDQVLVVGCPKVYDLRREYEFPPFAAAKVTFCGYISREPGRRTRAAMRQELGLIDDR
                     PLVLVTPGGGEDGYQLVATTLQSLASLPLRDCPRTHIVCGPEMNAEQRAHVHRSVLSM
                     PNVSAQDFSDDMMSLMGAADVVLAMGGYNTVCELLTLRKRAVLVPRMKPGVEQLIRAE
                     RMSTLGFVRMVHPQSLTPDSLLTALECELAAWQAHRSPPPLRRLDGLARVTEALFATM
                     GHRAPAARRVEQIDRNPATSTHATQAHARAVEEQRAA"
     gene            12265..13575
                     /locus_tag="PKV56_12495"
     CDS             12265..13575
                     /locus_tag="PKV56_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HOX68678.1"
                     /translation="MALLPPDHPDRLALASEVHARPPEPLETPSRVTYVAVLIDAAAR
                     EQELEHIEALCSRYGVEPPAPGSTHFSAGLDTLRFKWERHGEFSGYTFSTTGRSDSPF
                     EQTAVSRLPAGWLEKIPGATLVAAHAKLVPAGSSPPEASLLSIFFGGNIPVGAEIGDG
                     SGFAFTDFRVHADGYARFVVFDRHLTPRQAGRTLQRLFEIEVYRMLALLALPIARRQS
                     PRIFGIEGSLAELTEGIARDQRTDDESLLQALTRLAAEVESELASSQFRFGACRAYYE
                     LVTTRIGELREQRMSGIQTIEEFMARRFTPAVATCNTVSQRLHGLSERVAQTSALLST
                     RVDITRERQNQALLASMDRRAKLQLRLQQTVEGLSVAAIVYYVAGLVGYVSKGLKAGG
                     LHIEPDLVVGIAIPLVALGALWTIRRAKRRIAAAEAGQPPAREL"
     gene            13652..>14115
                     /locus_tag="PKV56_12500"
     CDS             13652..>14115
                     /locus_tag="PKV56_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HOX68679.1"
                     /translation="MSEQFPFSAIVGQDEMKLAILIAAVDPSVGGVLVFGDRGTGKST
                     AVRALAAMLPKMRAVIGCRYHCDPQGSAPVCEECRAFKLAGTPVKSHLIPVPVVDLPL
                     GATEDRVVGALDLERALSQGVKAFEPGLLARANRGFLYIDEVNLLEDHLVDLL"
BASE COUNT         2382 a         4618 c         4824 g         2291 t
ORIGIN      
        1 cacgccagcc atcacgatgt ccagagcggt gttgtagccg cactggctga tcgaggcgcg
       61 ggctgagcgc atctcggcca ccatgtcgct cacatgtctc acgagctcga cgccggcttg
      121 tccggcaccc tggcgctgga ggtcctgcca gtgctcctcc ggcaggaagg gtccggcgat
      181 cactcgcatg gccaacccga ggcgcatccg ggcgcgggct tcgagcaccg tgtggaagag
      241 ctgctggccg acgatgcccc cgcctgccga gatcagcagc cgttcctgcc gcttcagatc
      301 tgacccgccc tgtcgggtgg gcacgacgta gccgctgtga aagacaggcg tgcgcatcgc
      361 ccgtgacggg cggaaggtgt cttcgaggcg agcaaagccc gcgtcggcgt gcacgatgac
      421 cgtgtcgaaa tagcggtcgg tcaaccagcg tgcgcggtcg tcgtggtgct tctggtccgg
      481 gcgggcatcg acgaggatgt cgcgcaggct gcacgccacc agcgccgggc ccttcgactg
      541 acgcttcgcc gcgcggatca tcggcaggat ctcgttggcg aatttcttgc gcccgaacgg
      601 aaacagctcc accagcagca catccggccg cgccgacacc acggctgcat cgatcagacg
      661 ccgccgctcg gtgcgcgctg caaccacgtc caggctgcgg tcgcggctca ccaccgtgtg
      721 cccgtcgtcc atgccgagcg gcgggaggtc gatgagttcg atgccggcgg gcacctgcac
      781 gccgggaggc agtctgccgc cgttcaggaa caccacgcgg aactgcttgg tcagggcggc
      841 tgccagcgcg aagctgcgtg tcagatggcc gatgcccagc gagtgctggc agtagaacag
      901 cagggtgggg aagtgaatct caggcatggc gacgctccac gagggccagg taggttccgc
      961 gcgaacgcat cagggattcg tgagtgcctt gctcgaccag acgaccgtcc tggaggacca
     1021 gcacgcggtc gtaggacgac atgtcggtca ggtcgtgcgc gatcacgacc agcgtgcggc
     1081 cccgatgcac tcgggccacc gactcgtgga tcaatcgggc cgaggtggcg tcgatcgacg
     1141 cggtgggctc gtccatcacc aggattgccg gttccttgac cagagcgcgt gccaggcaca
     1201 ggcgctgcct ctgcccgcct gacagcgagg cggcgcgttc gcccagctcg gtgtcgtatc
     1261 cctgtggcag gtcaactatg aattcatgcg cccgggccgc ccgcgctgcc gactcgatcg
     1321 cctcgggtgt tgcgtcaggt cgaccgtagg cgatgttctc gcgaaccgag acgccgaaga
     1381 gcaggttgtc ctgcagcacg atgccgatct cgcgccgcag gctttcgcgc gagtagcgtc
     1441 ggatgtcgat gccgtcgatc agtatgcggc cggcgctggg ctcgtacagt cgcagcagca
     1501 ggttcatcag cgtggacttt ccggcgcccg acggaccgac cagcgccacg cgttccccgg
     1561 cggcgatgcg caggttcaag cggtccagca ccggccgctc ggcggcgtag ccgaaactga
     1621 tgtcctcgaa cacgatttcg ccggcgggag agcgcagatc catcgcgtcg gctgcatcgg
     1681 ggcgatcagg ctccagatcg aggatctcgc tgacgcgctg ggcgctgaca ccagcacgcg
     1741 agaacttgcc ggccaggcga gcgaggtcgc gcaccggctt gtacaggctg gtcacgtagg
     1801 tgatgaacac cagcagttct ccgggggtga gccgcccggc cagcacctcg cgtgcgccca
     1861 tcaggatcgt gatcgccgta ccggcagcgc tgatgaccgc gatcgccttc acgatggcac
     1921 cgcttgctcg tgtcgaacgc atgccgcttt cgtagttcgc ctcgatctcg ctgcggaagc
     1981 ggtcttcctc gaatccctgg cgaccgaagg cctgcaccag tgcgatcgag ctgagcactt
     2041 cgttgagccg cgacgccatg cgtccctcgt gcctgcgctg ctggcgcacc gaatccttga
     2101 cgcgccgatt gagcacgtag atcactccca gcagcggagg cagcgtgaca gccgccacca
     2161 gcgccagctg ccagttcagc atgaacatga cggccagcat cgccaccagc gtgacggcgt
     2221 gccgccccag ggtcagtgcc cattcggaga acatgtcgcg cagcaggttg gtgtcgccgg
     2281 ccaccttggt gatcagctcg ccggtgcgct gtgcacggtg gtgggcgagc gacagtcgct
     2341 gcagatgcgt gaacagttcg ccgcgcagac gccacgtgat gcgatgcccg acgcgcgcgc
     2401 tggtgtagag ctgcagatac gacagcgcgc cggacgccag cgcgatggca gcgatcgccc
     2461 ccgcagcgct cagcagcgcg ggccaggtcc cccagtccag caggggctgc aaccacgaaa
     2521 gtgccgacgg caacggcttc gccagcagga tgtggtcgaa gatgaccttc agcggccacg
     2581 gcgccagcac ttcggcgagc accacaccgc acaggccgaa tgcggccagg cccagggcca
     2641 ctcgctgttc cctcagcgag gcgcggacga tcgagcgcag gcgggcgccc ggcaacgagg
     2701 cctgttcggt catggtgagc actccagcaa gagttgatcg gccagcgact cggcgcggct
     2761 gagccggctt tcgatgtgtg aacgccagtc aggaacctgg aacacgaagg cgcggcccgc
     2821 ctgcagcagc tgctgaaccg cgagatgcca gtgcagacgt cgtccgtcga atcgggttgg
     2881 tgcgtggcgt gagtaggcgg cgagcatcgc ggtcgcgaaa gccggtccgc ccgacaacag
     2941 cgagaccttg gtgacgaacg cagcgaggtc ctccatcgga tcgcccaagg cgaactcgtc
     3001 gaagtcgaac agcaccacct gcccgtgcgc cacccagaac tgctcggggt ggcagtcgcc
     3061 gtggatcaga ccgatgggcg gctcgggcag ctgccgggaa gccatctcga gcgcgtcggc
     3121 gagccgcgcg actcgcagtt ccagttccgg gaccgtgcgg gatatcttct tctgcctgcg
     3181 ccgcacttcg gtcaacgcat gaacgcggtc ccgtggcagc ggaccagcca gcgacagcgg
     3241 ggcggcgtgc atcagcgaca gggcgtgcgc gacacggtcc ggcaggcccg cttcgccaga
     3301 cccgcccacg atctgaacca gcggaacgcc gggagcctgt gattgccaga acgtgtgcgt
     3361 ctcggggtga tgggccaggg gctgcgccac gaccggaaac tgcgcgccga gttgagacat
     3421 cgtccagaag tagcgaaagc gctcgtggac agcagaggcc cgaccgtcgc agaaggtctt
     3481 cgcatagagg tgttcctcgc ctttcgaggt tcgccggctg tagcgcagcg tcgcccggtc
     3541 ctcaggcacg tgacgcagtg ccacgacctg ctcggcggcc ttgccccacc atgggcaggt
     3601 ctgttgcaga tcgagcaggg cgggcagttg cggcaaaccg ggatccgccg gccagcgcca
     3661 cagcagcagg tcgagcgacg gcacgcactg cacctggatg tcgttggcga cgtctgatgc
     3721 agtgccgtcg cgatagacct tgccgtagta cgtctcggtg ctcgttcgcc cgctggcgag
     3781 gtcgcgcagc gacagctcat aacaaagcgt cagtggattc gggttgcgct gccgagagga
     3841 gcttcgccgc actttcgaaa tgctgatgga atcgatcgac agcgctccgc tggcgcaggc
     3901 gggcagggtc ttctgcagca cggcttgcat ggcccgggcg tcgagacagg tctcgatgcg
     3961 ttgatcgacg gcgagcgctt ctgtcacacc acctccgcct gacgctgaaa cgcgaggtca
     4021 ctggccagcg cgatcagggc cccgacgcgc tcgaaacggc ccggcttgag gttcaccaca
     4081 cagcgccaca cccgctgcgt cagcaggttc cacgcgatgg cccagcccag ccccgggagt
     4141 tcgaagcgcg acccgccggc atcggcatag ccgtccagca gtgcctgcca cgcgctctgg
     4201 tcgcgcagcg gatcggcatc gtcgagcagc gcgcgataaa ggccttcggc cacccaggct
     4261 ccaagttcga gctctgccgg accgcgcacg acattgtcca gatcgatcag ggccgggccg
     4321 cagccgtcga caaggatgtt gagcgggtgc agatcgccat gcagcgtggc agggcggtcg
     4381 aacggcagcg cctgaaggcc ggcagcgagt cgggcggcaa gatgctggag tccggtctga
     4441 ctgtcgggca aggcgtcggt cagcaccgcc agcacgtcgg cgagacgctg atgcagcatc
     4501 gacggggtca gctcgcgggc gagcgagacc ggggtggcgt gcagggcggc cacttgcgcc
     4561 ccgagctgac gcgccgagat gaaggtccgc tgcggcggca cgtcgcgcag ggacacgccg
     4621 gcgaggcctt cctgccaatg aaatccggca ccaggctgcc agagcacggc cttcggtgtg
     4681 cgcagccgcc cgtcgcggca tgcggcgctg tgctgcagct cacgaaggat ggcgtggcat
     4741 gtttcgccat cgggttcccg cgtggacttg gcgtagaggc gcaccgactc aggggcctgc
     4801 gcgccgcatt gccactgcag gtcgacgcgt gcggtgaggc gctgctcggg cacgtactgg
     4861 gcgatctcga tcgaatgtcc gacgaggcgg ccgcggcccc cgctgagcgc cgacacgacc
     4921 tgcggcagga cctcgccgtg cagccgttcg acatcgctga gcacgtgcgt ggcgacgagc
     4981 ttgggatcgt tcggccacca ccaggtgagc agccccaggc gggggatcga ttgcagcgcc
     5041 ggtccggctc gcgaggcgac cagcggggcc acgggttcag cggctgcgcg ccgcgcgcgc
     5101 tggatcgcat cgtccccgca caagcgggcc gcgacccgct gctcgaaggc gcgcccgtca
     5161 tgcctgtcgc gcaatcgcag tcggtaggac agcgtgcagt tgcgccgggg ccggtacttc
     5221 acccggtcga cgatgcaacc ctcgaccacg aggccctgct tgtgcaacag ctcaccgaac
     5281 acctccgccg cgcggcgcgg gtcgaccacc acggccagat tcggcagcag cggatcggta
     5341 ggcagcgaag cattggtgct cacggggcct cgccgatctc gaccgcgacc gcttgcccgt
     5401 tcaggtcgcg cagggtggct cccgcgtggc tcagggcgcg aacgatgcgg ccatccgccg
     5461 tttcgcgcca gtggatccat cggcccttga aaacgtgcgt accgatgagc caggtttcgg
     5521 agtgcacccc gcgatcgtgg aaccacccgt cgtgcagtgt ctggccatcc tgggcaagac
     5581 ggatgcgcaa cgcgtgcccg gcgagaccct cgccgctgcg aaccacgcga tcctcgacct
     5641 gcaggtccgg ggcctcgcaa ccccacggga cgagcacggc atcgaaatcg gcgtcgcgac
     5701 cgcgtgcatg cgttctcagc accggggcgg catccttgtg tccataccgg gccgagaccc
     5761 aggctccggc aagttcggtt cgctgatccg gtcgagcggg cagcgcgatg ctcaagtgcg
     5821 ggctgtcgag gcgaagaccc gctgcgcgat gcagggaact ggcgctggca gctgccgtac
     5881 cgagctggaa actcacgaca tagtcgtgct cgccgggcgc acgcagccag tcgctcacga
     5941 tccagtagcg tcgatcgatg aacgcgatgc agcgatcgtg gacggcgtcg tactcgtggc
     6001 tgctgcagcg gccgtgcagc aggtcgagcg atccgccatc gtgcgtctcg atcagcaggg
     6061 tgtcgggtgg tggtccgctg atcttgtggc gcaccgcgcc ctgtgcgtgg cgcgaaggct
     6121 ccttgatggc ccggggtgag tagcgtgtct gcgatcgtcc atcgacacag accgtgctgt
     6181 gcgctgccgt gctgcggaag tgcacgcgcc aattggtctc tccagcttcg ctgtacgtgt
     6241 atcgccctgg atccacgacc aggggtcgac cgaaggcgaa caactcgaat gacagtgcat
     6301 cgaagtgccc atggttgccc tcgcccagcg gaccgcaatc gaacatcagg tgctgcgcct
     6361 cggcactccc gttggccggc cattcgctgc gcagcacgtg gtacccgctg ttggagaagt
     6421 gggcgttgcg ctgcaaggga gcgacgccgt cggccccgcc tgtcgcgacg aagagcaggt
     6481 ctggcctgcg atacaggtgg gcggcctgcc gcagcagcgc ccggtacccg cgtgcatcac
     6541 catcggagaa gctcggcgcg cttccgtcgg gctggtgcac gtgaacgctg aagtcgaatg
     6601 cccgcagcag cgcctcgtcc atcgcggcag gcacgccgtg gccgttgcgc gccgcgaggg
     6661 tgcgcacctg gaaccagttg cgcagcgcga ggtggtggta gtcggtggac agctcgcaat
     6721 gcacgccatc cggcagcaga tcggcgtgga tgttcaccag tgtctcgcgc agcgcgaacg
     6781 atctccagtg ctgcgcgcgc gcgaactccg ggaacaccac gccggcgaga aacaccgcca
     6841 gcagttcgag cgtgcggtga ttccgcttgg gtgtcaggtt cgcacacagg aactcgacct
     6901 gcgcatgaat cgattgcagc aggcgtcgga agaaggccgg atcgacgggc gcctcgcgac
     6961 cgtccattcc cggcagaacc agtccttgca ggctgtagat ccagttctgc acgcgccgac
     7021 cggtgacgtc tgccgcgatg aagcccggcg gcgtgacccg catccagcca tcgatcagct
     7081 cggcccagcg tctcacgtaa cgcaggtctg cgctgcgctg ccacgcctgc gacaggccgg
     7141 cggcgtaata gaacttgtgc agcaggatgt gccactcgat gtcggcgctc gggttctgca
     7201 gccaatcgat cgggtcgccc agcgagtggg tttctccgtt gaactcgaag cggccgtgca
     7261 tcaaaccttc aaggcgcttc ggcagcacct cctcgggatc cacgatcgcg tcgaacacca
     7321 caccgcgatc gctgcgatag tgggcgagca gcgcggcatc gtccagcccg caccattcgg
     7381 gcaggaacag cgcgtcggac tcgggctctg cagcgagcct gtgggctgcg gggaacaacg
     7441 cgctcaaggg gtcaccttcg cagcgacagt cgtccgagca tcgctcgtcg ggctgcgcaa
     7501 ctgcttcatg aacaggtcgc gcagcagcac cgtggttcgt cgagagtcga aacgatcgca
     7561 gatccgcgcg cgggcagaca tcgacagggc ctgatgcaga gccgcgtcgg tcagcaggcg
     7621 gccgagcgct tgcgaaagag cgacaggatc cctgggcggg accagcagcc cgtgtacgcc
     7681 gtcgtcgatc atctccggga tgccagagat cgcggtggac accaccggga tgcccatggc
     7741 catcgcttcg gcgagcacgt tggggaaacc gtcccggtct ccgtcctcca tgatctggca
     7801 gggcagggcg aaggcatgag cccgggcata gatgtcgcgc agtccggcct gggtcactgc
     7861 gccgtgcagc ttcacccggt cttcgagccg cagctgctgg atcaggctgc gcaccgtgga
     7921 cgaggcattg ccttcctcgc cgacgatcag gcaatcgaaa tcgacactgg cgtcgcgcaa
     7981 ccgggcgcag gcctcgatca gttgatcgaa gcccttcttt tcgaccaggc ggcccaccga
     8041 caggatcaac gggcgggtgt tcccggatcc ggcggcacgg cccgtcggtg cgaaccattc
     8101 ggtgtcgagg ccatggtaga tggcgtgcac ttcctcgggc cgtggatggc gctcccgcag
     8161 cacctgcgcg ttggcgcagg tgcaggtggc gacgaagcgc gcaccatgca tcttgcgttc
     8221 gaggaggtcg ccaggattca gttcggcctg gtagatgtcc ttggcgtgcg ccgtgaagct
     8281 gtagcccagg ccgcacatgc gggcggccag ccaggtgatg gtggccacgc cgtggcagaa
     8341 gtggccgtgc aggtgcccga cctgcccttg gcgcaacacg tcggctgcga tttccccggc
     8401 ctgcaggaac tccttgatga acaccttgcg caggcgcagg cggccggatt cgtcgcggct
     8461 gcggtgttgc catgccagcg ccagcgcaga ccccagtgct ccagcccagc gcacggggtg
     8521 gcgcaccacg accctggcgt gcgagcgcca gaacgcgggc aggttctgct tcagccagcc
     8581 cggcaacgag gtgcccgaca gtgaagaagc gcggggcagg tacgtgagcg gtgcgcgaat
     8641 cgcttcgacc accggatgca cgacggactc gttctcccgc ttgaccgaat aaaggcgcag
     8701 cggaagcccc agccgttcga gctggtggat ctcgtgcgtg atgaacgttt cggacagcct
     8761 gggaaagccg ttcatcacat aggcgacgct gggttggttc atggcggtgt caggcggcga
     8821 cgcaagaggc ggggtgggca tgtccgcctg ccagcaactg gatcaccagg cgcaggttgc
     8881 ggtcgttgtc gaaatgcgtg gtcacggtgg accgggcttc gcgagccagc gcggcccgca
     8941 gctcgggcgc ctcgacgagc ttctcgatcg catcggccag tgcggcagcg tcgtcgggct
     9001 cgaccagcca tccgttgtca cggtgcctga caacctcggg gatgcccgaa acgcgcgttg
     9061 ccaccaccgc cacgcccatg gccatggcct ccagcagcac gttcggaatg ccgtcgcggt
     9121 cgccgtcggc ggccacgcgc gacggttgca catagaccgc ggcccgcgca taccgatcga
     9181 tcacctgctc gcgcgtgagc ttgcccacga gctgaacctg gtcggcgagc gccaggtcct
     9241 cgatcagcgc agacagcggg gccttctctt ctccgtagcc cacgatctcg cagcggtagg
     9301 gaacccctcg gctggtcagc agcatgcaag ccttgatgag ggtgtcgagc cccttcttct
     9361 ggcgcaaccg gcctacgctc aggatcagcg gcaccagagc gggcgtctgg cggtgcagcg
     9421 ggttgaacac gcccgcgtcg atgccgtgat acatccgatg gacattcgcg ccgggggcca
     9481 tcgcgctcag cgtccgacag ttgtagtcgg tgcaggtgac cgtgaagcgc gcggcagtca
     9541 tcttgcgctg cagatccgcc gggttggaca ggtagatgtc cttcgcgtga gcggagatcg
     9601 agaaagggat tccgccgagc gtcgacacca gttcggacac atcggccggc gtcgagatga
     9661 aatgggtatg caggtgcgcc acgccgtcct ggcgcaactg gccgaccagc cagcctgcgc
     9721 gaacgaagtc gcgcagacct tgccgttggc gcgacagggc atggcgaagc gacttggcgt
     9781 accgccaggg tgaagtcgcc gcgagcacgc agtgccgcca cagcagagcc ggggcgtggc
     9841 gccacgtggc agcaggcacc tgcacgacct gggcgcggac acgttccacg acgggatggg
     9901 ccactgcatc ggtcggggcc gccaatgtgt aaagccggat cggcacgccg aggcgctcga
     9961 gcccgagcac ttcctcgagg acgaaggtct cggagagctt gggaaatatc ttgaccagca
    10021 gcccgatcga tccgggttgg tcgtgcgggg atgcgctcat gagccggccc gcgtgcgcaa
    10081 cggcagcatg cgcagcaagg tgtcatcccg ccggatgaag tgatgagcca gcgcactggc
    10141 tgcatggaag ccgatgagga cgtagccgca tcgcccggca atctcgtggc actccttgac
    10201 aagcgaggcg agctccttgt cgggcgcgac cagcggcatc agctcgaggc cgaagaacgg
    10261 aatcggcttg ccggcggcgc tcagcatcaa ccagccggcc agcggcatca ccagcagcca
    10321 ggcataaagc agcgcatgca ttgctgtcgc ggcatgctgc tgccagcgtg gcggtgcggg
    10381 ctggatcggt ggcgtggtct ggccgaaccg ggcaaagagg cgcagggtgg tcagtgccag
    10441 cacggtcagg ccgagcaggg catgtccgcc cttcaacgct tcgcggggat ccgatccctt
    10501 ggcgaacaga acgcgcagtt cgatgcacga gaacacgcct atcagcacgg ccagcatgac
    10561 ccagtgaagg tgccgcgtga gcggcgcgta attcgtgctg gctcgcgtgg ccttcatcac
    10621 gcggcccgct gctcttcgac ggcgcgcgca tgcgcctggg ttgcgtgcgt gctcgtggcc
    10681 ggattccgat cgatctgttc gacgcgccgg gccgccggag cccggtggcc catcgtggcg
    10741 aacaacgcct cggtgacgcg tgccaggcca tcgagacggc gcagcggcgg cggtgaacga
    10801 tgcgcctgcc acgccgccag ctcgcactcc agtgccgtca ggagggagtc tggagtcaag
    10861 gactgggggt gaaccatgcg gacgaatccg agcgtcgaca tccgctcggc gcggatcagc
    10921 tgttcgacgc cgggcttcat tcggggaacc agcacggccc gcttgcgcag ggtgagcaac
    10981 tcgcacaccg tgttgtagcc acccatggcg aggaccacgt cggcagcgcc catcagcgac
    11041 atcatgtcgt cgctgaagtc ctgggcactg acattgggca tcgacagtac cgagcgatgg
    11101 acatgggccc gttgttccgc gttcatttcc ggcccgcaga cgatgtgcgt acgcgggcag
    11161 tcacgcaagg gcaggctggc gagcgactgc agggtggtgg cgacgagctg gtagccatct
    11221 tcgccaccac cgggcgtgac gagcacgagc ggtctgtcgt cgatcaggcc gagttcctga
    11281 cgcatcgctg ccctcgtgcg gcgtccgggt tcacgggaga tgtagccgca gaaagtgacc
    11341 ttggcagccg cgaatggcgg gaattcgtat tcgcgccgca ggtcgtagac cttggggcac
    11401 ccgaccacca gaacctggtc gtagtaggcc gtgatcgctt cgaagtagcc gttcttgcgc
    11461 cagatccgcg tggtcgattc agcactgtcc agaatgtccc gcaacagcag gaccagcttc
    11521 gggcggactt ccgccgaggg caggccctcg agtgcaccgg cgagttcatc ctcgactccg
    11581 aatggcttct tgtcgaccag gatcagatcc ggtgcgaagt cggtgatggc cgagcggatc
    11641 agattcgcgc gcaggcgcac ggtcgcctcc agtgtcatgg gcaggctgcg ggcgccgtac
    11701 ttgccatcca cgccgcgcga caggcaaggc agcttgatgt agtcgattcc ctcggggatg
    11761 cggaaggcgt gcagcatcgg cgagcccgtg atgaccagca ccgaaacctc gggcgaactt
    11821 tgaacgaggt ggcgagccac ttcgagcatg cgacggatgt tgcccagacc gaaggtgtcg
    11881 tgcgaataga tcaatacgcg tttcatggca ggctttctgg ttgcggcgcc agtgctgtgc
    11941 gcaacgcctg tgccaaatgc gtcactaatc gcaagtcttt gatttcattg gttcttacgc
    12001 tctgttacgt gggtgttgac cgcactttct gggcgatttc acccagtctt gtcggtccac
    12061 atgccccagt ggaacccgat gaactgggag ccgacccggc ggttgcttca acgggatcga
    12121 tgccctggca actgcgcacg caagcgccgt ccgcacaggc tgtcgtcgga cgacgttgaa
    12181 gacgtcgaag cagccggttt cgtactcgac ggcatactgg agtgcatctg cggatcgcat
    12241 ccagccgccc gtcgaaagga caccatggcc ctgcttccac ccgaccatcc ggaccgactc
    12301 gcgctcgcca gcgaggttca cgcgcgtccg cccgagccgc tggaaacgcc gtcgagggtg
    12361 acgtacgtcg cggtgctgat cgacgccgca gcgcgagaac aggagctcga gcacatcgag
    12421 gccctgtgtt cgcggtatgg cgtcgagcca cccgcgccgg gctccacaca cttcagcgca
    12481 ggactcgaca cactgcgctt caagtgggag cgtcacggcg agttctccgg ctacaccttc
    12541 agcaccacgg gacgcagcga ttcgccgttc gaacagaccg cggtctcgcg gctgcctgca
    12601 gggtggctgg aaaagatccc cggtgcgacg ctggttgcgg cgcatgccaa gctcgttccg
    12661 gctggcagca gtccgcccga agcgtcgctg ctgtccatct tcttcggcgg aaacattccc
    12721 gtgggtgcgg agatcggtga tgggtccgga ttcgcgttca ccgacttccg cgtccatgcg
    12781 gacggctatg cgcgattcgt cgttttcgac aggcacctga cgcctcgcca ggcgggccgc
    12841 accttgcagc ggctgttcga gatcgaggtc tatcggatgc tcgccttgct ggcgctgccg
    12901 atcgcgcgcc ggcagtcgcc gcgcatcttc gggatcgaag gttccctcgc cgagctgacc
    12961 gaaggcatcg ctcgcgacca gcgcaccgac gacgagtcgc tgctccaggc gctgacccgt
    13021 ctggctgcag aggtcgagag cgagctggca tcgagccagt tcaggtttgg cgcctgccgt
    13081 gcctactacg aactggtgac cacccgcatc ggcgaattgc gggagcaacg gatgtccgga
    13141 atccagacga tcgaggagtt catggcccgc cgcttcacgc ccgcggtggc gacctgcaac
    13201 accgtgtccc agcgattgca cggtctgtcg gagcgcgtcg cgcagaccag cgcgctgctg
    13261 tcgacccgcg tcgacatcac gcgcgagcgc cagaaccagg cgctgctggc atcgatggat
    13321 cgccgcgcca agctgcagtt gcgccttcag cagacggtgg aaggcctgtc tgtcgcggcc
    13381 atcgtctatt acgtcgcggg tctggtgggc tatgtctcga agggcttgaa ggccggagga
    13441 ctgcatatcg agcccgacct ggtggtcggc atcgcgattc cactggtcgc gctgggcgcg
    13501 ctgtggacca tccgtcgggc caagcgtcgc attgccgcag ccgaagcggg tcagccgccc
    13561 gcgcgcgagc tgtgagggag tgctgatccg aatcgtcggg ctgcgggccg tgtgctaacg
    13621 tgatttgtcg cggtcttgca gttccctgga tatgtctgag cagttccctt tttccgccat
    13681 cgtcggccag gacgagatga agctcgccat cctgatcgcg gccgtcgatc cgagtgtcgg
    13741 tggcgtgctc gtcttcggtg atcgaggcac cggcaagtcg actgccgtgc gggcactcgc
    13801 tgcgatgctc cccaagatgc gtgcggtgat cggctgccgc taccactgcg atccccaggg
    13861 cagcgctccg gtctgcgagg agtgcagggc attcaagctg gcgggcacgc cggtgaagtc
    13921 tcacctgatc ccggttccgg tggtcgacct gccgctgggt gccaccgaag accgtgtggt
    13981 cggcgctctc gatctcgaga gggcgctctc gcagggcgtc aaggctttcg agcccggcct
    14041 gctggcgcgc gcgaatcgcg gcttcctcta catcgacgaa gtcaacctgc tcgaggacca
    14101 cctggtcgac ctgct
//
LOCUS       DAOORV010000094        14074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5290_length_14074_cov_9.678008, whole genome shotgun sequence.
ACCESSION   DAOORV010000094 DAOORV010000000
VERSION     DAOORV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5290_length_14074_cov_9.678008"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKV56_12505"
     CDS             <1..308
                     /locus_tag="PKV56_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68680.1"
                     /translation="IDDIDEAIVSRCIALIRYHSPDQLARERIWRVMAEQFGLLLDDA
                     LISTLAACYPQASGRDIKGLAKLVAKFCIHKAAQPTLEVFARCAIFRALDAQLVEAD"
     gene            308..664
                     /locus_tag="PKV56_12510"
     CDS             308..664
                     /locus_tag="PKV56_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68681.1"
                     /translation="MYHRSLYDIVMETGRSIGVLTREMQGEDELFASALTLRAVEAQL
                     LIMAHTLGNVTPALHQRLIQVDWIGWAALHDKLRNGVQPRREEIWYAVNGLVPQTMAL
                     LLELRRKQPVWFEVGY"
     gene            846..2231
                     /locus_tag="PKV56_12515"
     CDS             846..2231
                     /locus_tag="PKV56_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-peptide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HOX68682.1"
                     /translation="MKHYARSRAWCEWDDFDAWQLRAMQALSDPDVAALSPFHLLSLP
                     GITAAQQQACATRWMADRLLASAVDRALLSAEFAAGSVALALQTAAPHDRIRIGYLSC
                     DFHQHATALLMVEMLEAHDLDRFELHAYSYGADDGLGMRQRLTRPFERFNDITDLDDL
                     QAARAIHADGIDILVDLKGYTAGTRTALLTYRPAPLQVSFLGYPGTLGGTFCDYLVSD
                     RFVTPASAAAGYSEALAWMPHSYQPHGRGADIGPAPTREQAGLPPEGLVLCCFNQAYK
                     FTPEVFDIWCDLLAQTPDSVLWLLRTEQAEGNLRREAMQRGVAPHRLVFADDLPQAEH
                     LARLQLADLVLDTSPYNAHTTASDALWAGVPLVTCAGDTFASRVAGSLLHAVGLSELV
                     THSLGEYADLAAALVADRQRLRTLRDQLARERLAAPLFDVAAYTLDLESLYVGMWERH
                     CAGMPPQALGT"
     gene            complement(2274..2918)
                     /locus_tag="PKV56_12520"
     CDS             complement(2274..2918)
                     /locus_tag="PKV56_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68683.1"
                     /translation="MTLMFYMTPGSCSTGIHILLEELELPFEVTVLNLPAGDHRRPDY
                     LALNPKGSIPALRLDDGRVLTEFQAIAYWLACRHPRARLLPSDPLQTALAIELMDHVV
                     GTVHGQGYTRIFTTDSYLPPGLDSAQRMHWQQAIAERGREIVSLGFEIIDGRLPDEGY
                     AAGSDFSIADAALFYVEFWADKIGLALPARCAAHYQRVRARPVVQRVLREEGYR"
     gene            3049..4611
                     /locus_tag="PKV56_12525"
     CDS             3049..4611
                     /locus_tag="PKV56_12525"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOX68684.1"
                     /translation="MLADDSLLNDDDLLRWRACERRFWLSRRVASDRPGPSAFAVRAE
                     LTGEAAVVRGPQPGDALRATFPGAIVIEAPTTPADWREAVQRTSACLRDKRMQAEGRA
                     LFGACLASDDGVQVRIDVLAPGEHGLRMFKVRHATVGDESDVDDVALWVHVAARCGLR
                     LVNVGLLLVDTDFVYPGHGCYAGLFREVDLTPVLGSRPVARWLVEMRTCDRGAEPAAE
                     PGAKCRLHGGCDFIEHCRLRAPAAAGAGAETAGPAQLEIVGRELAAELRLEGHADLYS
                     VLPERLADARHRRAVRAIQQRAPVLEPSVAALMRAHPYPRCTLRFDTIGFAVPVWSGT
                     QPYQVLPFQWTCDVEPAQGERVHRAFLADRQGDPRRAFAQSLLQALGSSGPVFAYNAG
                     FERNRIRELAQCFEDLSDALEALLPRIVDLFQVARTHYYHPAMRGSWSFKSVFRTIAP
                     ELDATRFDCDGEPSAQAAFAHSLQPGIDAPTRERLREALRLHGERQVAALHRMVSMFE
                     QADGAAGPAPVD"
     gene            4749..5459
                     /locus_tag="PKV56_12530"
     CDS             4749..5459
                     /locus_tag="PKV56_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68685.1"
                     /translation="MNVMTRLTAVDRWNLLVTGLMGPRPGLFFEALRARGTLKCLLPE
                     LDALFGVPQLCDGPEPVDTGVHQLRLVDLTARALAPLDVRFAALMHKIGKAGTPHDML
                     PSHPGHEARGLALLDGLAQRIAVPGSALDLARLVISECDHVHRASDLRAGAIASLLER
                     LQAQAQPERFEQLLCVCSCDYAAYRGHAAIDYPKAPRLRRAQAAAAQADVTGLLPESA
                     LYARAQAITQALRGACPA"
     gene            5466..7274
                     /locus_tag="PKV56_12535"
     CDS             5466..7274
                     /locus_tag="PKV56_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOX68686.1"
                     /translation="MTNHLHGATSVYLQQHAGQPVDWYPWGAEALTRAQREDRPILLS
                     IGYAACHWCHVMARESFADAQTAALMNDGFINIKVDREERPDLDQIYQQAHQLLRRAN
                     GGWPLTVFLSPQGVPFYSGTYFPHQAQADQPAFSDVLARVRSVWGERRSDLAQQDRAL
                     MAALAASQPRAQGVAIPDAAVRDAARDQLASAFDAVHGGFGGAPKFPHPSDLAFLLRR
                     GRDEGDAQALHMALFTLRKMAEGGLYDQIGGGFFRYSVDAQWRVPHFEKMLCDNGLLL
                     ALYADALALTDEPLFRRVVEDSAGWLLREMRAVSGAFCASLAADDARGREGLAYVWES
                     ELLRLALSPNEWDVCAAHWGLVDAPAFEGRYWQLRVARPAGALAATLRRPEAMVDELI
                     ASARAKLLTAREKREAGERPARDDKQLTGWNALVITGLARAASVYGRTDWLQAARAAR
                     VWVQAERWHMGRLSMLPGQDAFLDDHAFLLEAVLALHEIDPQADDLPFAAAIADAMLA
                     QFEDPADGGFFFTRHDAPALIHRSRSGIDAALPSGNGTAALALQALGRRLDDPRYFAA
                     ADRCVRVFAAAVRRDPASHTRLLQAAMRLQLQKASA"
     gene            7343..7747
                     /locus_tag="PKV56_12540"
     CDS             7343..7747
                     /locus_tag="PKV56_12540"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOX68687.1"
                     /translation="MLTRLIYASEISTPLTSSAVQEIVDKARNANQRRHLTGMLAFDS
                     RSFLQALEGPRDMVSELFCKIAADPRHQRVQLLEVVPVDERQFSAWTMGFAAADAHGH
                     ETYLRYSSSDRFEPLQMTASGALGVLRALASR"
     gene            complement(7784..8221)
                     /locus_tag="PKV56_12545"
     CDS             complement(7784..8221)
                     /locus_tag="PKV56_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HOX68688.1"
                     /translation="MDLVYKAEMLSQGGRSGTIQSDDGSVKIKLAVPASMGGKGDGPN
                     PEQLFAGAFAACFEHSVRHIAKKSHVPLRGCYVEAALSMFVTDEDAYRMALTLTVHMA
                     GALEQADADNLLERAKGICPYVDATKNNVTLNLQAVLDTPVTA"
     gene            complement(8237..8473)
                     /locus_tag="PKV56_12550"
     CDS             complement(8237..8473)
                     /locus_tag="PKV56_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOX68689.1"
                     /translation="MPLYDYHCKSCDAEFELLVRSSTVPTCPKCGATELERAVSRIAP
                     AGKIEAIRASNRRQAAREGHFSNFSAAERAKLTK"
     gene            complement(8486..8926)
                     /locus_tag="PKV56_12555"
     CDS             complement(8486..8926)
                     /locus_tag="PKV56_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOX68690.1"
                     /translation="MAHVVFFEKPGCAGNARQRKLLEVAGHTLDRRDLLTAPWTRDAL
                     LAFLAPLPVAQWFNRAAPRIKSGDVVPEQLSAESALTQLLAEPLLIRRPLMQRTDSGA
                     RLVGFDAAEVDRFVGLAGVPASPAAASLEGCAAAPTHPRCQSTD"
     gene            complement(8940..9665)
                     /locus_tag="PKV56_12560"
     CDS             complement(8940..9665)
                     /locus_tag="PKV56_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOX68691.1"
                     /translation="MNDVARRFHRLTAQEAPAWLAAHADALLLDARDAREHALGHLPG
                     SVRLDGRNHEALLLGEPKARPVFIYCYHGNASRTYAQMFADFGFHDVADLIGGWEAWK
                     QQGTAMTPAQAVGAHGNTPLMQAAWRGDMPAVESLLAQGASLNDTNGDGNNALWLACV
                     SNQPELVTRLVEAGVPIDHQNLTGATCLMYAASSGKPEIVRTLLQLGANPHLLTQDDY
                     SALDMAASIECLQLLRTTSRATA"
     gene            complement(9662..11296)
                     /locus_tag="PKV56_12565"
     CDS             complement(9662..11296)
                     /locus_tag="PKV56_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HOX68692.1"
                     /translation="MSTSQDTVRAYHERSKHQLRRYAAGPETLDWDAQPDPFRRWAGS
                     SLTMLPLVAASIGTTWSELHGDVAPLGISRETIGALFELSFGLTAWKQLGPDRWALRA
                     NPSSGNLHPTEAYLLAAQVRDLADGLYHYAPKEHALELRTPLVASDPNGAPQLWIGLS
                     SICWREAWKYGERAFRYCQLDTGHALGALRYAAATLGWQARVVTHIGHAELAALLGLD
                     REMDFGTAEAEEPEVLVQIGPHLPLQLVVPRGWTNTDHWQGRANRLDVHPMYRWPVIE
                     EVAVATRDAGGASPADSTPEPSRPAQARVAAKADRPAVELILQRRSAQHFESAATMSM
                     GAFLRIVESLQPCPGLPWDTMPSAPLVHAVMYVHRVDGLPPGAYVLPRSASGADLLSG
                     ALTAGRAWEAVSQVPPGVPLQRLASPSRLAETLRTLSCHQAIARDACFAVSLVAEVGT
                     VFSGSPSGYRQLFRECGLIGQVLYLSAEAEGYRGTGIGCYFDDAVHEFLGLADQRLQV
                     LYHFTVGMPLSDTRISLEPPYADRSPITAPHDALAP"
     gene            complement(11405..11995)
                     /locus_tag="PKV56_12570"
     CDS             complement(11405..11995)
                     /locus_tag="PKV56_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68693.1"
                     /translation="MTTIDQTALGHLEAEGRLLNSVLKGPTKKAGRIGFRGDIALKFQ
                     AKMADEARPPEISSDQVIAAAQEGEAHICFLAGFLLSFEYLKLLTEVLGDSLSPKGKY
                     FLFCDNIDISKRYQVEYGGASFYVLPIDEATVYNETLQLLYLEKNDLKKLDTAGKVDA
                     IVDKAMSFDQSFPKLSFEEGLKLMGPVRNIYENRPV"
     gene            complement(12010..12438)
                     /locus_tag="PKV56_12575"
     CDS             complement(12010..12438)
                     /locus_tag="PKV56_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HOX68694.1"
                     /translation="MEAKPTVLESNRHYVRLGGHEAVVRLVDAFYLAMERLPQAATIR
                     AMHEPDLAQTRSVLVKYLTEWMGGARLYSPEHGAPMLRRRHQRFAVDSDARDAWMLCM
                     REALNQVCDDGALRLELETAFYKVADFMRNTESHSSPPHH"
     gene            12818..13699
                     /gene="nifH"
                     /locus_tag="PKV56_12580"
     CDS             12818..13699
                     /gene="nifH"
                     /locus_tag="PKV56_12580"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HOX68695.1"
                     /translation="MAKLRQIAFYGKGGIGKSTTSQNTLAALSDLGQKILIVGCDPKA
                     DSTRLILHAKAQDTILSLAAEAGSVEDLELEDVMKIGYKDIRCVESGGPEPGVGCAGR
                     GVITSINFLEENGAYDGVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
                     AANNISKGILKYANSGGVRLGGLVCNERQTDKELELAESLAKMLGSRLIHFVPRDNIV
                     QHAELRRMTVLEYAPESKQAQEYRTLAEKIHANAGNGTIPTPITMDQLEDLLMEHGIM
                     KAIDESQVGKTAAELVA"
     gene            13760..>14074
                     /locus_tag="PKV56_12585"
     CDS             13760..>14074
                     /locus_tag="PKV56_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOX68696.1"
                     /translation="MSMTVEDRKAANKALIDEVLKAYPEKMAKRRAKHLGQFEEGKPD
                     CGVKSNIKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYI
                     GIT"
BASE COUNT         2252 a         4700 c         4759 g         2363 t
ORIGIN      
        1 gcatcgacga catcgacgag gccatcgtct cgcgctgcat cgcgctgatc cgctatcact
       61 cgcccgatca gctggcgcgc gaacgcatct ggcgcgtgat ggccgagcag ttcgggctgt
      121 tgctcgacga cgcgctgatc tcgacgctgg ccgcctgcta cccgcaggcc agtgggcggg
      181 acatcaaggg gctggccaag ctcgtggcga agttctgcat ccacaaggcc gcgcagccga
      241 cgctggaggt cttcgcgcgc tgcgcgatct tccgcgcgct cgatgcccag ctggtcgagg
      301 ccgattgatg tatcaccgat ccctctacga catcgtgatg gagaccggcc gttcgatcgg
      361 tgtgctgacc cgggagatgc agggtgagga cgagctgttc gccagcgcac tgacgctgcg
      421 cgccgtcgag gcgcagttgc tcatcatggc gcacacgctg ggcaacgtca cgccggcact
      481 gcaccagcgc ctgatccagg tcgactggat cggctgggcg gcgttgcacg acaagctgcg
      541 caacggcgtg cagccgcgcc gcgaggaaat ctggtacgcc gtcaacgggc tggtgccgca
      601 gacgatggcc ttgttgctcg aactgcggcg caagcagccg gtgtggttcg aagtcggcta
      661 ttgactcggc gggccggcgc tggacgacgc atatcccacg gcgtcgtcga gcgcgtggat
      721 ggggcgaaag gaccgaccgg ccgcgtcacc gcgtgcccct tgggctccat ggcgtcgcct
      781 actgcacggc gcagaacttg cggcagggga gcatggccat tccctcgcgt ccgagcgacg
      841 cttcgatgaa gcactacgcc cgcagccgcg cctggtgcga gtgggatgac ttcgacgctt
      901 ggcagctgcg tgcgatgcag gctctgtcgg atcccgatgt ggcggctttg tcgcctttcc
      961 atctgttgtc gctgccgggc atcacggcag cgcagcagca ggcctgcgcc acgcggtgga
     1021 tggcagatcg tctgcttgcg agcgcggtcg accgggccct gctgtcggcc gagttcgcgg
     1081 ccgggtccgt cgcgctggcg ctgcagacag ctgctccaca cgacaggatc cgcatcggtt
     1141 acctgtcctg cgactttcac cagcacgcca ccgcgctgtt gatggtcgag atgctggaag
     1201 cccacgatct cgatcgtttc gaactgcatg cctacagcta cggcgcagac gacggactgg
     1261 gcatgcgcca gcgcctgacc cgaccgttcg agcgtttcaa cgacatcacc gacctcgatg
     1321 acctgcaggc ggcgcgcgcg atccatgccg acggtatcga catcctggtc gacctgaagg
     1381 gctacacggc gggcacacga accgcgctgc tcacctaccg gccggcgccg ctgcaggtga
     1441 gctttctggg ctatcccggc acgctgggcg gcacgttctg cgactacctc gtcagcgacc
     1501 gcttcgtcac gccggccagt gccgccgcgg gctacagcga agcgctggcc tggatgcctc
     1561 acagctacca gccgcacggt cgaggtgccg acatcggccc cgcgccgaca cgcgaacagg
     1621 ccggtctgcc ccccgagggc ctcgtgctgt gctgtttcaa tcaggcctac aagttcacgc
     1681 ccgaggtctt cgacatctgg tgcgacctgc tggcgcagac gccggacagc gtgctgtggc
     1741 tgctgcgcac cgagcaggcc gaaggcaacc tgcgccgcga ggcgatgcag cgcggcgtcg
     1801 cgccgcaccg cctggtcttc gccgacgacc tgccgcaggc cgaacacctg gcgcgccttc
     1861 agctggcgga cctggttctc gatacctcgc cctacaacgc ccacaccacc gccagcgatg
     1921 cgctgtgggc cggcgtgccg ctggtgacct gtgctggcga caccttcgca tcgcgggtgg
     1981 cgggcagcct gctgcacgcg gtcggcctgt cggaactggt cacgcacagc ctgggcgagt
     2041 acgccgatct cgccgcagca ttggtggccg accggcagcg cctgcgcacg ctgcgcgacc
     2101 agctggcgcg ggaacgtctc gcagcgcctc tgttcgatgt ggcagcctac acgctggacc
     2161 tcgagtccct gtacgtcggc atgtgggagc ggcactgtgc gggcatgccg ccgcaggcgc
     2221 tgggaacctg acagatcgtg cagcccgggt ctgcgcgatc gacctgacgg tgcctatcga
     2281 tagccttcct cgcgcagcac gcgttgcacc accggccggg cgcgcacgcg ctggtagtgc
     2341 gcggcgcagc gcgcaggcag ggccaggccg atcttgtcgg cccagaactc gacgtagaac
     2401 agcgctgcat cggcaatcga gaagtcagac ccggccgcgt agccctcatc aggcagacgt
     2461 ccatcgatga tctcgaatcc caggctgacg atctcgcgcc cgcgctctgc aatcgcctgc
     2521 tgccaatgca tccgctgcgc agaatcgagc ccgggcggca ggtagctgtc ggtcgtgaag
     2581 atgcgcgtgt agccctggcc gtgaacggta cccaccacat ggtccatcag ctcgatggcg
     2641 agcgcggtct gcaacggatc cgacggcagc agccgtgcac ggggatggcg gcaggcgagc
     2701 cagtacgcga tggcctggaa ctcggtcagc acgcggccat cgtccagacg cagcgccggg
     2761 atcgagccct tgggattcag cgccaggtag tcgggacggc ggtggtctcc tgccggcaga
     2821 ttcagcaccg tcacttcgaa gggcagttcg agctcctcga gcaggatgtg gatgccggtc
     2881 gagcacgacc ccggcgtcat gtagaacatc agcgtcatga aggcctcgtt gcaagatgca
     2941 tgacaacctg cgccgccccg acgaatctgc ctctgcacgg cgattgctac agggaagaat
     3001 cgacctccgt ctggcgtgac ctcgtgttgc gtttgtctgc ttatgcccat gcttgccgac
     3061 gactccctgc tgaacgacga tgacctgctg cgctggcgtg cctgcgaacg gcgcttctgg
     3121 ctgagccgcc gcgtggcgag cgaccgtccc ggtccctccg cgttcgccgt ccgcgccgag
     3181 ctcaccggcg aagctgcggt ggtgcgaggc ccgcagcccg gtgacgcctt gcgagccacg
     3241 tttcccggcg ccatcgtcat cgaggcgccg acgacgccgg ctgactggcg cgaggccgtg
     3301 cagcgcacgt cagcctgcct gcgcgacaag cgcatgcagg ccgaaggccg agcccttttc
     3361 ggagcctgcc tggcgagcga cgatggcgtg caggtgcgca tcgatgtcct ggcgcccgga
     3421 gaacacggac tgcggatgtt caaggtccgc cacgccacgg tgggtgacga atccgatgtg
     3481 gacgacgtgg cactctgggt gcacgttgct gcgcgttgcg gcctgcgcct ggtgaatgtc
     3541 ggcctgttgc tggtggacac cgatttcgtc tacccgggac acggctgcta tgcagggctc
     3601 ttccgggaag tggatctgac accggtgctg ggatcgcgcc cggtggcgcg ctggctggtg
     3661 gagatgcgca cctgcgatcg cggcgccgag cctgcagccg agccgggtgc gaagtgccgg
     3721 ctgcacggcg gttgcgactt catcgaacac tgtcggctgc gggcacctgc tgccgcgggg
     3781 gcgggcgcag agaccgccgg cccggcccag ctggagatcg tcggacgcga actggcggcc
     3841 gagttgcgcc tggagggcca tgccgatctt tacagcgtgc tcccggagcg cctggccgac
     3901 gcacggcatc gccgcgccgt gcgcgccatc cagcaacgcg cgccggtgct ggagccttcg
     3961 gtcgctgcgc tgatgcgcgc gcacccttac ccgcgctgca ccctgcgctt cgacaccatc
     4021 ggtttcgccg tgccggtgtg gtccggcacg cagccttacc aggtgctgcc gttccagtgg
     4081 acctgcgatg tggagccggc gcagggcgag cgagtgcatc gtgcattcct cgccgatcga
     4141 caaggcgacc cgcgccgcgc atttgcgcag tcgcttctgc aggcgctggg cagcagcggg
     4201 ccggtgttcg cctacaacgc cggcttcgag cgcaatcgca tccgcgagct cgcacagtgc
     4261 ttcgaggacc tgagcgatgc gctcgaagcg ttgctgccgc gcatcgtcga cctgttccag
     4321 gtggcacgca cgcattacta ccacccggct atgcgcggct cgtggtcgtt caagtcggtg
     4381 ttccgtacga tcgcgcccga gctggacgcg acccgcttcg actgcgacgg cgagccctcg
     4441 gcgcaggcgg ccttcgccca ttcgctgcaa cccgggattg acgcgcccac gcgcgagagg
     4501 ctgcgcgagg cgctgcgact gcacggcgaa cgccaggtcg cggccctgca tcgcatggtc
     4561 tcgatgttcg agcaagcgga cggcgctgcg ggtcctgcgc ccgtggactg acttctcgcg
     4621 ggcagccgcc cgtccgcacc ggcggccgat ggcacgcttc ctgcgcctcg ccgaggatgg
     4681 cgaccaggat caccgaggcg ggagcgcgtt tgtcgaacag gcgacaaagc cggacattcg
     4741 gcgaaccgat gaatgtgatg acccgcctga cggcggtcga ccgatggaac ctgctggtca
     4801 ctggcctgat gggcccgcgc ccgggcctgt tcttcgaggc cctgcgcgca cgcggcacgt
     4861 tgaagtgcct gctgcccgaa ctcgacgcgc tgttcggcgt gccgcagctc tgtgacggcc
     4921 ccgagccggt cgacaccggt gttcatcaac tgcgactggt cgacctgacg gcgcgtgcgc
     4981 tggctccgct cgacgtgcgc ttcgcagcgc tgatgcacaa gatcggcaag gccggcacgc
     5041 cgcacgacat gctccccagt caccctggac acgaggcgcg tggtctcgcc ttgctcgacg
     5101 gcttggcgca gcgcatcgcg gtgcccggct ccgcgctcga tctggcacgt ttggtgatct
     5161 ccgaatgcga ccatgtgcac cgtgccagtg acctgcgggc cggggcgatc gcctcgctgc
     5221 tcgagcggct gcaggcgcag gcgcaacccg aacgattcga acaactgctt tgcgtgtgca
     5281 gctgcgacta cgcggcctat cgtgggcatg ccgccatcga ctatcccaag gcaccgcgcc
     5341 tgcgccgggc gcaggccgct gcggcacagg ccgatgtcac cggcctcctg ccagagtcgg
     5401 cgttgtacgc acgtgcccag gccatcaccc aggccctgcg cggtgcctgc ccggcctgaa
     5461 ctgccatgac caaccacctg cacggcgcga catcggtcta tctgcagcag cacgccgggc
     5521 aaccggtcga ctggtacccg tggggcgccg aggcgctgac ccgtgcgcag cgcgaggacc
     5581 gtccgatcct gctgtcgatc ggctacgcgg cctgtcactg gtgccacgtg atggcgcgcg
     5641 agtctttcgc cgatgcgcag accgcggcgc tgatgaacga cgggttcatc aacatcaagg
     5701 tcgaccgcga ggagcgcccc gacctggacc agatctacca gcaggctcac cagttgctgc
     5761 gccgcgcgaa tggcggttgg ccgctgaccg tcttcctgtc gccgcagggt gtgcccttct
     5821 acagcggcac ctactttccg catcaggcgc aggcggacca gcctgccttt tcggatgtgc
     5881 tggctcgcgt gcgcagcgtc tggggcgagc gccgctccga tctggcgcag caggaccgcg
     5941 cgctgatggc cgctctggcg gccagccagc cgcgtgcgca gggggtcgcg atccccgacg
     6001 ccgcggttcg cgacgctgcg cgtgatcagc tcgcgagcgc gttcgatgcg gtgcatggtg
     6061 gcttcggcgg tgcgccgaaa tttccgcatc cgagcgatct ggccttcctg ctgcgtcgcg
     6121 ggcgtgacga gggcgatgcg caggcgctgc acatggcact tttcaccctg cgcaagatgg
     6181 cggagggagg cttgtacgac cagatcggcg gcggattctt ccgctacagc gtcgacgcgc
     6241 agtggcgggt tccccacttc gagaagatgc tgtgcgacaa cggcctgctg ctggcgctct
     6301 acgccgatgc gctggcactg accgacgagc ccctgttccg tcgcgtcgtc gaggactccg
     6361 cgggctggct gctgcgcgaa atgcgcgcag tttcgggggc cttttgcgcg tcgctggccg
     6421 ccgacgatgc gcgcggccgc gaggggctgg cctatgtgtg ggaatccgag ctgctgcgcc
     6481 tggcgctgtc acccaacgaa tgggacgtct gcgcagcgca ctggggtctc gtcgatgccc
     6541 ccgcattcga aggccgctac tggcagctgc gggtggcccg acctgcggga gccctggccg
     6601 ccaccttgcg ccggcccgaa gcgatggtcg acgagctgat cgcctctgcc cgcgccaagc
     6661 tgctgaccgc acgcgaaaag cgcgaggcag gcgagcggcc ggcacgcgat gacaagcagc
     6721 tgaccggctg gaacgcgctg gtgatcaccg gcctggcccg tgccgcctcg gtgtacgggc
     6781 gcaccgactg gttgcaggcg gcgcgtgcgg cacgggtgtg ggtgcaggcc gagcgctggc
     6841 acatggggcg actgtcgatg ctgcccggcc aggacgcctt tctcgacgac catgccttcc
     6901 tgctcgaagc cgtgctggcg ctgcacgaga tcgacccgca agccgatgac cttccgttcg
     6961 cggcggccat cgccgacgcc atgctcgcgc agttcgagga cccggccgac ggcggcttct
     7021 tcttcacccg ccacgacgca ccggccctga tccaccggtc caggagcggc atcgatgcgg
     7081 ccttgccgtc gggcaacggc accgccgcgc tggccctgca ggcgctgggc cgccggctcg
     7141 acgacccgcg ctacttcgct gccgccgatc gttgcgtgcg ggtctttgcc gccgcggttc
     7201 gacgcgatcc ggcgagtcac acccgtctgt tgcaggcagc gatgcgactg cagctccaaa
     7261 aggcctcagc ctgagacggg gcgagccgaa ataacggcat gccgtgctcg atcacggccg
     7321 tccgattcaa ccaggtacca ccatgctcac tcgcctgatc tacgccagcg agatctccac
     7381 cccgctgact tccagcgcag tgcaggagat cgtcgacaag gcccgcaatg caaaccagcg
     7441 tcgccacctc accggaatgc tggccttcga cagccgcagt ttcctgcagg cattggaagg
     7501 cccgcgcgac atggtcagcg agctgttctg caagatcgcc gccgaccccc gacaccagcg
     7561 tgtgcaactg ctggaagtgg tgcccgtcga cgagcgccag ttttcagcct ggaccatggg
     7621 ctttgccgcc gccgatgcgc acggccacga gacctacctg cgctattcga gcagtgaccg
     7681 gttcgagccc ttgcagatga ccgccagcgg tgcgctgggc gtgttgcggg cgctggcttc
     7741 acgctgagac atcaggtccg tctgatcctg cgggccggcg cactcatgcg gtcacgggcg
     7801 tgtcgagcac ggcctggagg ttcagcgtga cgttgttctt ggtcgcgtcg acgtaagggc
     7861 agatgccctt ggcgcgttca agcaggttgt ccgcgtcggc ctgctccagc gcgccggcca
     7921 tgtgcacggt cagggtcagg gccatgcggt aggcgtcttc gtcggtgacg aacatcgaca
     7981 gcgcggcctc gacgtagcag ccacgcaggg gcacatggct cttcttcgcg atgtggcgaa
     8041 ccgagtgttc gaagcacgcc gcgaaggcgc ctgcgaacag ctgttccggg ttcggcccgt
     8101 cgcccttgcc gcccatcgat gcgggcaccg caagcttgat cttgacgctg ccgtcatcgc
     8161 tctggatcgt gccgctgcgg ccgccctggc tcagcatctc ggccttgtag acgaggtcca
     8221 tgtcctgctc cctggttcac ttggtcagct tggcccgctc ggccgcgctg aagttgctga
     8281 aatggccttc gcgcgcagct tgccgccggt tcgatgcacg gatcgcctcg atcttcccgg
     8341 ccggtgcgat gcgcgagacc gcgcgctcga gctcggtcgc accacatttc ggacaggtcg
     8401 gcacggtgct cgatcgcacc agaagctcga actcggcgtc gcaggacttg cagtggtagt
     8461 cgtagagcgg catctgcggt gctcctcaat cggtggactg acatctcggg tgggtgggcg
     8521 ccgccgcgca gccttccagc gacgcggcag cgggcgatgc aggcacgcct gccagcccga
     8581 cgaagcggtc gacctcggcg gcgtcgaagc cgaccaggcg ggcgccgctg tcggtgcgct
     8641 gcatcagcgg gcgtcggatc agcagcggct cggccaatag ctgcgtcagc gctgattcgg
     8701 ccgacaactg ctcgggcacg acgtcgccgc tcttgatgcg tggtgcagcg cggttgaacc
     8761 actgagccac cggcaacggg gcgaggaagg cgagcagggc gtcgcgcgtc cacggcgcgg
     8821 tgaggaggtc gcggcgatcg agcgtgtggc ccgcgacctc gagcagcttg cgctgcctgg
     8881 cgttgcctgc gcagcccggc ttttcgaaga agacgacgtg cgccatctct ggacccgatt
     8941 caggccgttg cccgggaggt cgtgcgcagc agctgcaggc actcgatgct ggcggccatg
     9001 tcgagggcgc tgtaatcgtc ctgcgtcagc agatgcgggt tcgctccgag ttgcagcagc
     9061 gtgcgaacga tctccggctt gccgctggag gcggcgtaca tcaggcaggt ggcaccggtc
     9121 aggttctggt ggtcgatcgg cacgccggcc tcgaccagtc gcgtgacaag ctcgggttga
     9181 ttcgaaacac aggccagcca aagcgcgttg ttgccgtcgc cgttggtgtc gttcaggctg
     9241 gcgccctgtg cgagcaggct ttcgaccgcc ggcatgtcac cccgccaggc cgcctgcatc
     9301 aacggtgtgt tgccatgcgc gccaactgcc tgcgccggtg tcattgcagt gccttgctgc
     9361 ttccaggcct cccagccgcc gatcaggtcg gccacgtcgt ggaagccgaa gtcggcgaac
     9421 atctgcgcgt aggtgcggct ggcgtttccg tgatagcagt agatgaagac cggacgcgcc
     9481 ttcggctcgc cgagcaacag ggcctcgtgg ttgcgcccgt ccaggcggac gctgccgggc
     9541 aggtggccca gcgcgtgttc ccgggcgtcg cgcgcatcga gcagcagcgc gtcggcatgt
     9601 gctgccagcc aggccggcgc ttcctgcgcg gtcaggcgat gaaaccggcg ggcaacgtcg
     9661 ttcatggtgc gagcgcgtcg tggggtgcgg tgattgggct gcggtcggcg tacggtggct
     9721 cgagcgagat cctcgtgtcg gacaacggca tgccgaccgt gaagtggtag agcacctgca
     9781 ggcgctggtc agccaggccc aggaattcgt ggacggcatc gtcgaagtag caaccgatgc
     9841 cggtgcctcg gtagccctcg gcctcggcgc tcaaatacag cacctgaccg atcaggccgc
     9901 attcgcggaa cagctggcgg tagcccgatg gggaaccgga gaacaccgta cccacctcgg
     9961 cgaccaggct cacggcaaag cacgcatcgc gggcgatggc ctgatggcag ctgagcgtgc
    10021 gcagggtctc ggccaacctg gacggggagg ccagtcgctg cagcggcacg ccgggcggca
    10081 cctgcgaaac cgcctcccaa gcgcggccgg ccgtcagtgc tccggacagc agatctgcgc
    10141 cggatgcgct gcgcggcagg acgtatgcgc ccggcggcag cccgtcgacc cggtgcacat
    10201 acatcacggc atgcacgagc ggcgcggagg gcatcgtgtc ccacggcagg cccgggcagg
    10261 gctgcagcga ctcgacgatg cgcaggaacg cacccatcga catcgtcgca gcggactcga
    10321 agtgctgtgc gctgcggcgc tgcaggatca attcaactgc aggccggtct gcctttgcgg
    10381 cgactcgcgc ctgcgcgggc cggctcggtt cgggcgtgct gtcggccggc gatgcgcctc
    10441 ccgcatcccg cgtggcgacc gccacctcct cgatgaccgg ccaccgatac atcggatgca
    10501 cgtcgaggcg gttggcccgg ccctgccaat gatcggtgtt cgtccatccc ctcggaacca
    10561 ccagctgcaa tggcaggtgc gggccgatct gaaccagaac ctctggctcc tcggcctcgg
    10621 cggttccgaa atccatctcg cggtcgaggc cgagcagcgc ggcgagttcg gcgtggccga
    10681 tgtgggtgac gactcgagcc tgccaaccga gcgtcgccgc ggcatagcgc aacgcgccga
    10741 gtgcatgccc ggtgtcgagc tggcagtaac ggaacgcgcg ttcgccgtac ttccaggcct
    10801 cgcgccagca gatcgagctc aggccgatcc acagctgggg tgcgccgttc ggatcgctcg
    10861 cgaccagcgg cgtgcggagt tccagcgcat gttccttcgg cgcgtagtgg tacaggccgt
    10921 cggccaggtc gcgcacctgc gcagcgagca gatacgcctc ggtcggatgc aggttgccac
    10981 tcgacggatt cgcgcgcagg gcccatcggt ccggccccag ctgcttccat gccgtgagcc
    11041 cgaagctgag ctcgaaaagc gctccgatcg tctcgcggct gatgcccaac ggcgcgacat
    11101 caccgtgcag ctcggaccac gtggtgccga tcgatgcggc gaccagcggc agcatcgtca
    11161 gggacgagcc agcccatcga cgaaacggat cgggctgagc atcccaatcc agggtctcgg
    11221 gtccagcggc gtagcggcgc agctggtgct tgctgcgttc gtggtacgcc cgcaccgtgt
    11281 cttgcgacgt gctcatctcg tcttcctgcc tgtccatgtc gcaggcgctc gtgtccgcag
    11341 gaccgcgaac ggaggtgttc gcggtccccg gcgcaacgaa ggcttcggaa gccgcggatc
    11401 catgtcagac cgggcggttc tcgtagatgt tgcgcaccgg tcccatcagc ttgaggcctt
    11461 cctcgaagct gagcttcggg aagctctggt cgaagctcat cgccttgtcg acgatcgcgt
    11521 cgaccttgcc cgccgtgtcg agcttcttga ggtcgttctt ctcgaggtag agcagttgca
    11581 gcgtctcgtt gtagaccgtc gcctcgtcga tcggcagcac gtagaacgac gcgccgccgt
    11641 actcgacctg gtaccgcttg gagatgtcga tgttgtcgca gaacaggaag tactttccct
    11701 tcggtgacag cgagtcaccg agcacctcgg tgagcagctt caggtattcg aagctgagca
    11761 ggaaaccggc aaggaagcag atgtgtgcct cgccttcctg ggccgcagcg atcacctggt
    11821 cgctgctgat ctcagggggg cgggcctcgt cggccatctt ggcctggaac ttcagggcga
    11881 tgtcgcctcg gaagccgatg cgaccggcct tcttggtcgg gcccttcaac acgctgttga
    11941 gcaggcgtcc ttcggcctcg aggtggccga gagcggtttg atcgatggtg gtcatggtgt
    12001 gcgtcttctt cagtggtggg gaggggagga atgggactcg gtgttgcgca tgaaatcggc
    12061 gaccttgtag aaggcggtct cgagttcgag gcgcagcgcc ccgtcgtcgc agacctggtt
    12121 cagggcctcg cgcatgcaga gcatccaagc gtcgcgggca tcgctgtcca ctgcgaagcg
    12181 ctggtggcgc cggcggagca tcggtgcgcc gtgttcgggg ctgtagagcc ttgcaccgcc
    12241 catccattcg gtcaggtact tcaccagtac cgaacgcgtc tgcgcgagat cgggttcgtg
    12301 catcgcgcgg atcgtggcgg cctgcggcag gcgttccatg gccagataga acgcatcgac
    12361 gagtcgaacg accgcctcgt ggccgccgag ccgaacgtag tggcggttgc tttcgagaac
    12421 ggtgggtttc gcttccatgg gggcggttac tgcaaccgcc gtaccagcac acgcggcgga
    12481 cattcaagcc actgtccgcg caggggaatg cgtcgtgcag ggccctcctc gagcgtgtgc
    12541 cgacagcgtt cagggcggct tgctttgtcg ggttccgaac aaattccacg gaccccgccg
    12601 gaaggccgaa aaagcggctt ttccctcgtg aaaacaagca cttggatgcc gtgaagatgc
    12661 atggcacgcg cattgcgaag aagaggctgc gcggacccca tgtcaggcac cgagggacct
    12721 tcataagaag acccatacag gcaccgaaga ctgcatcgag atcgcgacga ccgcttgtgt
    12781 gaatcgtcaa cctctatcaa ttcattggag taatgaaatg gcaaaacttc ggcagatcgc
    12841 cttctatggc aaaggtggca tcggcaagtc caccacctcg caaaacaccc tggccgctct
    12901 gagcgacctg ggccagaaga tcctgatcgt cggctgcgac cccaaggccg actcgacccg
    12961 cctgatcctc catgcaaagg cgcaggacac catcctgtcg ctggctgccg aggccggctc
    13021 ggtggaggac ctcgagctcg aggacgtcat gaagatcggc tacaaggaca tccgctgcgt
    13081 cgagtccggc ggcccggagc ctggtgtcgg ctgcgctggc cgcggcgtga tcacctcgat
    13141 caatttcctt gaagagaacg gcgcctacga cggcgtggac tacgtctcgt acgacgtgct
    13201 gggcgacgtg gtgtgcggcg gcttcgcgat gccgatccgc gagaacaagg ctcaggaaat
    13261 ctacatcgtg atgtcgggcg agatgatggc catgtacgcg gccaacaaca tctcgaaggg
    13321 catcctgaag tacgccaaca gcggtggcgt tcgtctgggt ggcctggtgt gcaacgagcg
    13381 ccagaccgac aaggaactcg agctcgccga atcgctggcg aagatgctcg gttcgcgtct
    13441 catccacttc gtgccgcgcg acaacatcgt ccagcacgcc gagttgcgtc gcatgacggt
    13501 gctcgagtac gcacctgagt ccaagcaggc ccaggaatac cgcacgctgg ccgagaagat
    13561 ccacgccaac gccggcaacg gaaccatccc caccccgatc acgatggatc agctggaaga
    13621 cctgctgatg gagcacggga tcatgaaagc gatcgacgaa agccaggtcg gcaagaccgc
    13681 ggcggaactc gtcgcctgac gcaaccgaga ggggtggcgc ttcggcgcca cccagcccag
    13741 cacccacgga gcacacacca tgagcatgac ggttgaagac cgcaaggccg caaacaaggc
    13801 cttgatcgac gaagtcctga aggcctatcc cgaaaagatg gccaaacgcc gcgccaaaca
    13861 cctcggccag ttcgaggaag gcaaaccgga ctgcggcgtc aagtccaaca tcaagtcatt
    13921 gcccggcgtg atgacgattc gaggctgtgc atacgccggc tcgaagggcg tggtgtgggg
    13981 ccccatcaag gacatgatcc acatttcgca cgggccggtc ggctgcggcc agtacagctg
    14041 ggccgcacgc cgcaactact acatcggcat cacc
//
LOCUS       DAOORV010000095        13994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5323_length_13994_cov_7.115145, whole genome shotgun sequence.
ACCESSION   DAOORV010000095 DAOORV010000000
VERSION     DAOORV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5323_length_13994_cov_7.115145"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="plsY"
                     /locus_tag="PKV56_12590"
     CDS             complement(<1..678)
                     /gene="plsY"
                     /locus_tag="PKV56_12590"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOX68697.1"
                     /translation="MESVLPLAAALVAYLVGSLSFAVLVSRAMGLGDPRNYGSGNPGA
                     TNVLRSGNKGAALLTLLLDALKGYMPVWAVVAFGEPYGLGEGTMALVGVAAFLGHLWP
                     VFFGFRGGKGVATAAGVLMALNPLLGLGTLATWLIIAYFFRYSSLAAIVSALFAPFYQ
                     LLIWGSGPIAIAAGVMGLLLVWRHGANIQKLMNGTESKLGQKAAPAAAAAVPRRRASR
                     GWSRLLPQ"
     gene            complement(779..1306)
                     /gene="ybaK"
                     /locus_tag="PKV56_12595"
     CDS             complement(779..1306)
                     /gene="ybaK"
                     /locus_tag="PKV56_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOX68698.1"
                     /translation="MSRKEHASETPATQLLKRRAVIYSEHLYDYVENGGTAESSRQLG
                     VDEHHVVKTLIMEDDTAQPLVVLMHGDLQVSTKNLARQIGAKKVQNCKPEVAQRHSGY
                     LVGGTSPFGTKKAMPIYVEATVLELPRIYINGGQRGYLVGIDPRVLVTLLGARPVQCA
                     TGGAVVPQGPLKSQR"
     gene            complement(1543..2037)
                     /locus_tag="PKV56_12600"
     CDS             complement(1543..2037)
                     /locus_tag="PKV56_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX68699.1"
                     /translation="MNPDTSAPFSIRAAELRDVNAIVQLIRELAEFEHLTHLLQVTPE
                     KLRPHLFGERPVAEALVAEGAGSVLGFALFFTNFSTFLAQPGLYLEDLYVRSDQRGRG
                     IGKALLSRVGRIAGERDCGRFEWSVLDWNESAIRFYEKMGATVMPDWRICRLTGDALQ
                     RLRG"
     gene            complement(2037..2303)
                     /locus_tag="PKV56_12605"
     CDS             complement(2037..2303)
                     /locus_tag="PKV56_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HOX68700.1"
                     /translation="MQWTERHSACWRKNLQITSLLLAIWLGVTFVLGFFARDLNFTFF
                     GWPFSFWVGAQGAPLVYVILVGYYAHRMERLDRQHGMSEDDNET"
     gene            2404..3508
                     /gene="prfB"
                     /locus_tag="PKV56_12610"
     CDS             join(2404..2484,2486..3508)
                     /gene="prfB"
                     /locus_tag="PKV56_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOX68701.1"
                     /translation="MDVEHINAIGNHLADLSKRTVDLRGYLDYERKALRLNEVNAALE
                     NPSVWNEPKRAQELGREKRTLEAVVQTIDHLTSNLADNTELYDMSKAEGDFDGLQAIE
                     ADAAKLEETVAQLEFRRMFNNPADPSNCFIDIQAGAGGTEACDWAGMLLRQYLKYCER
                     KGFKATLEDETAGDIAGIKSATIKVEGEYAFGLLRTETGVHRLVRKSPFDSAGGRHTS
                     FASLFVYPEVDDSIEIDINPADVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQC
                     QDGRSQHSNRDVAWKRLRSRLFDFEMRKRMEEQQKLEDTKTDVGWGHQIRSYVLDQSR
                     IKDLRTGYEVSATQKVLDGDLDPFIEASLKQGV"
     gene            3508..4188
                     /locus_tag="PKV56_12615"
     CDS             3508..4188
                     /locus_tag="PKV56_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOX68702.1"
                     /translation="MWRASAAMLFDLDGTLIDSMTQHQQAWTEWHRRHGLRIDESTFF
                     AATAGRSNPEILADLLPGESTDRYRAWADEKEALYREIARRTLTLVAGAEDFVRQARA
                     DGARLAVCTASTPENMALAFERFGIDSWVDCVVSPSDGLRGKPHPDIFLEAARRLGCA
                     AGDCVVFEDAPLGIEAARRANMQAVALTTTLPAAAFAGYDNLILVAPDFLAPSLLTPS
                     TDPTSSHA"
     gene            4181..6844
                     /gene="pepN"
                     /locus_tag="PKV56_12620"
     CDS             4181..6844
                     /gene="pepN"
                     /locus_tag="PKV56_12620"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOX68703.1"
                     /translation="MREGTAPLIRREDYTAPAFWIKSVDLTFDLDAAKTLVTSRMKLE
                     RNPAVPPQALKLHGEDLTVLRVLVNGESVSFRNEEGALVVDSLQDIGDGAFTLEIRNT
                     CSPERNTQLSGLYTSSGGLFTQCEAEGFRRITYFLDRPDVMAIYTVTLRASKAAYPVL
                     LSNGNLVEQTDLEGGRHQAVWHDPFPKPSYLFALVAADLVAREQHIRTRAGRDHLLQV
                     YVRRGDLDKTAHAMNSLIASIVWDEARFKLPLDLDRFMIVAVGDFNMGAMENKGLNIF
                     NTKFVLATPATATDVDYAGIESVVGHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQE
                     FSIDMAGSESARAVKRIEDVRQLRQLQFPEDAGPMAHPVRPDSYQQINNFYTATVYEK
                     GAEVVRMMQTLVGRDGFARGMTLYFERHDGQAVTCDDFAQAIADANPDSELARQLSQF
                     KRWYAQAGTPRLTAQGRFDAEARTYTLTVEQAQSGGEPFVVPLAMGLIARDGRALPLQ
                     LAGEGAAVGTHRVLVLNEARSSYTFTGIDSAPVPSLLRGFSAPVILGDDLNDTDLLVL
                     LKHDTDPFSRWEAGQRLALNRLLAATQAGEAQPLDNDFIEAMRDILRHPGLDAAFKEM
                     VLTPPSEGYVSEKLSVVHPQHIHTARESMRSQLAAALREDWAWAFEQHQVGGAYSPDP
                     VSSGKRALANLSLAMLCLDAAAQGDAVWPGRAYQRFKDAVNMTDRLGALSALVHSHAE
                     LATPALERFHAMFKGEALVIDKWFALQATAPEIDGRVFARAQLLLKHPDFSIRNPNRA
                     RSLIASLCMMNPAAFHRTDAAGYVFWADRVLELDAANPQLAARMARMLDRWSRLAEPY
                     RSAAREAISRVAAKSDLSSDVREIVSRALAS"
     gene            6855..7865
                     /locus_tag="PKV56_12625"
     CDS             6855..7865
                     /locus_tag="PKV56_12625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOX68704.1"
                     /translation="MSKRVSLTQYLVEQQREHGHIPGQLRLLIEVVARACKRISISVN
                     KGALGDVLGSAETENVQGEMQKKLDIIANEVLIEANEWGGHLAAMASEEMEGIYVVPN
                     RFPQGEYMLLFDPLDGSSNIDVNVSIGTIFSVLKREDSGRPVSEADFLQPGRQQAAAG
                     YCVYGPQTTLVLTVGDGVAMFTLDREQGSWVLTQDGLRIPEDTKEFAANMSNMRHWAP
                     PMRRYIDECLAGTEGVRGKDFNMRWIASMVADVHRILTRGGVFIYPWDKREPGKAGKL
                     RLMYEANPMGFLVEQAGGAATNGHQRILDIQPDALHQRVSVMIGSKNEVDRLTRYHQE
                     AD"
     gene            7908..7983
                     /locus_tag="PKV56_12630"
     tRNA            7908..7983
                     /locus_tag="PKV56_12630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7941..7943,aa:Thr,seq:cgt)
     gene            8138..8614
                     /locus_tag="PKV56_12635"
     CDS             8138..8614
                     /locus_tag="PKV56_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOX68705.1"
                     /translation="MNATRHSQSDEPGSSHSLPLLYNVVYCSRATAEVDDAAVARILE
                     TARQRNPVHGITGLLVFGGGIFFQWLEGPRDNVTQLMTNLKADPRHKDVVALTESEEA
                     RERLFPDWDMEFVTTDDIRDVLIDAMSTTEDAKHAEALKLLLVHLDSGELSGLTTA"
     gene            complement(8634..9974)
                     /locus_tag="PKV56_12640"
     CDS             complement(8634..9974)
                     /locus_tag="PKV56_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX68706.1"
                     /translation="MDLFSQRLLNDGVRKREVFGWAMYDFANSGYTTVVLTAVFNAYF
                     VSVVAQGAAWGTLAWTIAIAASSLIVMLTLPAIGAYADLRAAKKRLLGGCTVVCVLGT
                     AALAGAGPGDLGLAILAVVVSNIAYSYGESLTAAFLPELARPEAMGRVSGWGWSFGYL
                     GGMLALGLGLAYVLSAQARGEPATSFVPVTMLITAATYALASVATFMLLRERAVPQFI
                     AGRDQAGKVPSSLKSGGWGASFARLADTWRHARQYRDFSWLLACGACYQAGISVVVAL
                     AAVYAEQALGFKQADTMLLIFVVNIAAAGGAFAFGYWQDRIGHKRALSVTLLGWILMT
                     VLAVLARGPALFWVAAMIAGLCMGSSQSAGRAMAGLFTPGDRRAEFFGLWTFATRASA
                     VLGLLTYGLVTWLTEGNQRLAMGVTAGFFVGGLLLLAKVDMERGQRAAGPEQVP"
     gene            complement(10007..11305)
                     /locus_tag="PKV56_12645"
     CDS             complement(10007..11305)
                     /locus_tag="PKV56_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOX68707.1"
                     /translation="MDVVLLVFLIFLNALFAMSEMALTASRKARLQVMVETGDAGAVA
                     AMELHDDPTKFLSTVQIGITSIGVLNGIVGDAAFSAPFALWLHHTFDMHDNAAEILAT
                     AMVVVVITFLTIIFGELVPKRLGQMYPESVARLVAQPMNWLSMSARPFVMLLSFCTES
                     TLRLMGIRGGPSRSVTEEEIAASLEEGLDAGVIEAQEHQMVRNVFRLDDRQVGSMMIP
                     RAEIVWLDAVAPADAVLKVIAADEHSRYPVCRGGLDDVVGVVSAQSLLQQAIGGSVLS
                     ITERLQPAVFVPETLSGMELLDQFRASSAQLVFVVDEYGEVQGMITVRDVLEAITGEF
                     TTPSDDAWAVQREDGTWLFDGLIPVPELKDRLELKDLPEEGRGRYNTLAGMIMLLLGR
                     LPQTTDSVEWEGWRFEVVDLDGKRIDKVLASLSPTGDHDR"
     gene            11353..12177
                     /locus_tag="PKV56_12650"
     CDS             11353..12177
                     /locus_tag="PKV56_12650"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HOX68708.1"
                     /translation="MVYLIGDVQGCCGALQRLLDTIGFSPSRDHVIVLGDLVNRGPDS
                     LGTLRLLRDLGEAATCLLGNHDLHLLAVAHGIRKAHRSDTVSEILGAPDRDAWIDWLR
                     HRRMALHEHGWLMVHAGVPPPWTLQTTLQLAAEVEQQLRGPDLHGFLAVMYGNQPDRW
                     SDTLVGPDRLRFAVNALTRARFVAADGTLDFETKEGAGGAPPGLVPWFEHPKRRTAGV
                     PMAFGHWSTLGLIHRPDLLALDTGCVWGGLLSAARLVPGGRSEIIQVECEQAQRPG"
     gene            12232..12321
                     /locus_tag="PKV56_12655"
     tRNA            12232..12321
                     /locus_tag="PKV56_12655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12266..12268,aa:Ser,seq:tga)
     gene            complement(12344..>13994)
                     /locus_tag="PKV56_12660"
     CDS             complement(12344..>13994)
                     /locus_tag="PKV56_12660"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68709.1"
                     /translation="VAAGLDCGSTCSKVLNAGTSVTLTAVPATGSKFVGWSGACTGAS
                     LSCTVAMSQSRSVTANFGLLSFSLMVVKDGNGTGTVTSTPSGVSCGTTCSVTRTYGTL
                     LTLSAKPAAGSAFSGWGGACSGTGTCSVTLSQASRVTATFVRTHNTLTVSRSGTGTGG
                     VASSPAGINCGTVCTVPVTVETTVSLTPTPAAGSRFTGWAGACSGTGACEVSMGAART
                     VIATFTRNTYALDLARDGTGGGSVTSAPAGINCGATCRFVFNSLTVVKLTAKPVAGSA
                     FAGWTGACSGSSLTCSVTMNEAASVGATFSLTHYALSVTRNGTSTGTVTSTPAGINCG
                     TTCSKLMPLGSAVTLVPLPVAESAFSGWAGACTGTDPCTVTMSEPRAVAATFTRTHYT
                     LTVARTGTGSITSAPAGIDCGTVCKSSQPLNTTVPLTAVPADGMRLSGWSGSGCSGTG
                     TCTVTMSAARSVSATFVRITYPLTLTRTGSGTVVSSPAGISCGGTCTKAFNSGTAVTL
                     SAKPATGMVFSGWSGACSGTASTCRVSMSEALAVGATFAAP"
BASE COUNT         2563 a         4716 c         4582 g         2133 t
ORIGIN      
        1 ttggggcaga aggcggctcc agccgcggct ggcacggcgg cgcggcaccg cggcagccgc
       61 ggctggagcc gccttctgcc ccaacttgct ttcggtgccg ttcatcagct tctggatgtt
      121 cgcaccatgc cgccacacca gcagcaaccc catcacgccg gccgcgatgg cgatggggcc
      181 ggagccccag atcagcagtt gatagaacgg cgcgaaaagc gccgagacga tagcggccag
      241 ggacgaatag cgaaagaagt aggcaatgat cagccaggtt gccagcgtgc ccagcccgag
      301 caaagggttc agtgccatca gcacgccggc ggcagtcgca acgcccttgc cgcccctgaa
      361 tccgaagaaa accggccaca gatgtccgag aaaggccgcc accccgacca gcgccatggt
      421 gccttcgccc aggccgtagg gttcgccgaa cgcgaccaca gcccacaccg gcatgtagcc
      481 cttcaaggca tcgagcagca gcgtcagcag ggccgcgccc ttgttgcccg atcgcagcac
      541 gttggttgcg ccggggttgc ccgaaccgta gttgcgcggg tcgccgagcc ccatcgcccg
      601 gctgacgagc acggcgaacg agagggatcc gacgaggtag gccaccagtg cggcggcgag
      661 gggaagaacg gactccatga gcatctgaaa gtggcggcgg ccgcaattct gcgccctgcc
      721 cggcccgcgc tggcaggacg tgcgccaaag caggctcgct ggcgagcctg ccccggatct
      781 agcgctgaga cttcagcggc ccctgcggca cgacggcgcc accggtcgca cactgcacgg
      841 gccgggcgcc aagcagcgtg accagcaccc tcggatcgat gccgaccagg tagccgcgct
      901 ggcctccgtt gatgtagatg cgcggaagtt caagcaccgt cgcctcgacg tagatcggca
      961 tcgccttctt cgtgccgaac ggcgaggtgc cgccaacgag gtagccgctg tgtcgctgcg
     1021 caacctcggg tttgcagttc tgcaccttct tggcgccgat ctgacgcgcg aggttcttgg
     1081 tgctgacctg caggtcaccg tgcatcagca cgaccagtgg ctgggccgtg tcgtcctcca
     1141 tgatcagcgt cttgaccacg tggtgctcgt cgacaccgag ctggcgcgac gactcggccg
     1201 taccgccgtt ctcgacatag tcgtacaggt gttcggaata gatcactgcg cggcgcttca
     1261 gcagctgcgt cgcgggggtt tcggatgcgt gttctttgcg gctcatcggg aatcgaaatg
     1321 ggaaagggaa tgcagcttca ctgagccgta gctcgaagca caaagctgat cgcaacgctc
     1381 agatgctccg gcaacttcgt cgtgccccgc gcggtcggat tctcgtccgg ccgccgtctg
     1441 gcggtcgtca ccaccggaca gatcgaagcc acctcccgtg gtttgcagcg ctgcacgccg
     1501 tgcagtcaac tcgatcaacg aggcacccgt cgcgtccacc gctcagccgc gaaggcgttg
     1561 cagcgcgtcg ccggtgagcc ggcagatgcg ccagtcgggc atgacggtgg cgcccatctt
     1621 ctcgtagaag cggatcgcac tctcgttcca gtcgagcacg ctccattcga agcggccgca
     1681 gtcccgctcg ccagcgatcc ggccgacccg gctcagcaag gcctttccga tgccgcgtcc
     1741 acgctggtcg ctgcgaacgt aaaggtcctc caggtagaga ccaggctgtg cgaggaaagt
     1801 agagaaattg gtgaagaaca gcgcgaaacc cagcacgctc ccggcgcctt cagccaccag
     1861 cgcctcggcc accggccgct cgccgaacag gtgcgggcgc agcttctccg gcgtgacctg
     1921 cagcaggtgc gtcaggtgct cgaattcggc gagttcgcgg atcagctgca cgatggcatt
     1981 gacgtcgcgc agttcggcag cgcggatgga gaaaggggcc gacgtatcag gattcatcag
     2041 gtttcgttgt cgtcttcgga cattccatgt tgccggtcga gcctttccat gcggtgcgcg
     2101 tagtacccca ccaggatcac gtagaccagc ggcgcacctt gcgccccgac ccagaagctg
     2161 aacggccagc cgaaaaacgt gaaattgagg tcgcgcgcga agaagcccag cacgaaggtc
     2221 acgccgagcc agatcgccag gagtagcgat gtgatctgca agttcttgcg ccagcaggcg
     2281 ctgtgccgtt cggtccattg catcgcttcg ctctcctgcg ttgacccata gccctggcgg
     2341 cccgagtcta cgggggtcgc gtgagagaat gatgcatcca aaaattgcag aaatgagcgt
     2401 gtcatggacg tcgaacacat caatgccata ggcaaccacc tggccgacct gagcaagcgc
     2461 acggtcgacc tccgggggta tctttgacta cgaacgcaaa gcactgcgac tgaacgaagt
     2521 gaacgcggcg ctcgagaacc cgagcgtctg gaacgagccc aagcgggcgc aggaactggg
     2581 gcgcgagaag cgcacgctgg aagcggtggt gcagaccatc gaccacctga ccagcaacct
     2641 ggcagacaac accgagctgt acgacatgtc gaaggcggaa ggcgacttcg acggcttgca
     2701 agccatcgaa gccgatgcgg ccaagctcga ggagaccgtc gcgcagctcg aattccgccg
     2761 gatgttcaac aacccggccg acccgagcaa ctgcttcatc gacatccagg ccggtgccgg
     2821 cggcaccgag gcctgcgact gggcgggcat gctgttgcgc cagtacctga agtactgcga
     2881 gcgcaagggt ttcaaggcga cgctcgagga cgagaccgca ggcgacatcg cgggcatcaa
     2941 gagcgcgacg atcaaggtgg aaggcgaata cgccttcggc ctgctgcgca ccgagaccgg
     3001 cgtgcaccgc ctggtgcgca agagcccgtt cgattcggcc ggaggtcgcc acacctcgtt
     3061 cgccagcttg ttcgtctatc ccgaggtcga cgattcgatc gagatcgaca tcaacccggc
     3121 cgatgtgcga accgacacct tccgcgcctc gggcgccggc gggcagcaca tcaacaagac
     3181 cgattcggcg gtgcgcctga cgcacatccc gaccggtatc gtggtgcagt gccaggacgg
     3241 ccgaagccag cacagcaacc gggacgtggc ctggaagcgg ctgcgctcgc gcctgttcga
     3301 cttcgagatg cgcaagcgga tggaagagca gcagaagctg gaagacacca agaccgacgt
     3361 cggctggggc caccagatcc gcagctacgt gctcgaccag agccgcatca aggacctgcg
     3421 caccggttac gaggtgtcgg ccacgcagaa ggtgctcgac ggcgacctcg acccattcat
     3481 cgaagccagt ctcaagcagg gcgtgtgatg tggcgtgctt ccgctgccat gctcttcgat
     3541 ctggacggaa ccctgatcga cagcatgacg cagcaccagc aggcctggac cgaatggcac
     3601 cggcgccacg gactgaggat cgacgaatcc acgttctttg cggccacggc cggacgctcc
     3661 aacccggaaa tcctcgctga cctgcttccc ggcgaatcca cggaccgcta tcgggcctgg
     3721 gccgatgaaa aggaagcgct gtaccgcgag atcgcacgcc gcacgctgac gctggtcgcc
     3781 ggtgccgagg atttcgtgcg acaggcccgg gccgatggcg cgcggctcgc ggtgtgcacc
     3841 gcctcgacac cggaaaacat ggcgctggcc ttcgagcgct tcggcatcga cagctgggtc
     3901 gactgcgtgg tcagcccgtc cgacggattg cgcggcaagc ctcatccgga catctttctc
     3961 gaggctgcga ggcgcctcgg ctgcgcagct ggcgattgcg tggtgttcga ggatgcaccg
     4021 ctgggcatcg aggccgcacg tcgtgcgaat atgcaggcgg tggcgctgac caccacgctg
     4081 ccggcggctg cgttcgccgg ctacgacaac ctgatcctgg tggcgccgga tttcctcgcg
     4141 ccctcactcc tgacaccgtc caccgacccg acctcatccc atgcgtgaag gcactgcccc
     4201 cctgatccgg cgcgaggatt acaccgcgcc ggcgttctgg atcaagtccg tcgacctgac
     4261 gttcgacctc gacgccgcga agacactggt gaccagccgg atgaagctcg agcgcaaccc
     4321 ggccgtgcca ccccaggcgc tgaagttgca cggagaggac ctgaccgtgc tgcgggtcct
     4381 ggtcaacgga gaaagcgtgt ccttccgcaa cgaggaaggc gcgctggtcg tcgacagcct
     4441 gcaggacatc ggcgatgggg ctttcacgct ggagatccga aacacctgct cgcccgagcg
     4501 caacacgcag ctgtcgggcc tgtacacctc aagcggcggg ctgttcacgc agtgcgaagc
     4561 cgaaggcttc aggcgcatca cctacttcct ggaccgcccc gatgtgatgg cgatctacac
     4621 cgtcacgctg cgcgccagca aggccgcgta cccggtgctg ctgtcaaacg gcaacctggt
     4681 cgagcagacc gatctcgagg gcggtcggca tcaggccgtc tggcacgacc ccttcccgaa
     4741 gccgagttac ctgttcgcgc tggtggcggc ggatctggtg gcacgcgagc agcacatccg
     4801 cacccgcgcc ggccgcgatc acctgttgca ggtgtacgtg cggcgcggtg acctggacaa
     4861 gaccgcgcac gcgatgaact cgctgatcgc cagcatcgtc tgggacgagg ctcgcttcaa
     4921 gctgccgctg gacctcgatc gattcatgat cgtcgcggtc ggcgacttca acatgggagc
     4981 gatggagaac aaggggttga acatcttcaa caccaagttc gtgctggcca cgcccgccac
     5041 ggccaccgac gtcgactacg ccggcatcga gagcgtcgtc ggccacgagt acttccacaa
     5101 ctggacgggc aaccggatca cctgccgaga ctggttccag ctgagcctga aggaaggcct
     5161 gacggtcttc cgcgaccagg agttcagcat cgacatggcc ggcagcgaaa gcgcccgcgc
     5221 ggtcaagcgg atcgaggacg tgcggcagct gcggcagttg cagtttccgg aagacgcggg
     5281 gccgatggcg cacccggtgc gtcccgacag ctaccagcag atcaacaatt tctacaccgc
     5341 caccgtctac gagaaaggcg cagaggtcgt gcgcatgatg cagacgctgg tgggccgcga
     5401 cggcttcgcg cgcggcatga cgctgtactt cgaacgccac gacggtcagg ccgtgacatg
     5461 cgacgacttc gcgcaggcca tcgccgacgc caaccccgac agcgaactgg cccggcagct
     5521 ctcccaattc aagcgctggt atgcgcaggc aggcacccct cgactgacgg cacaggggcg
     5581 cttcgacgcc gaagcgcgca cgtacacatt gaccgtcgag caggcgcagt cgggaggcga
     5641 accattcgtc gtgccgctgg ccatggggct gatcgctcgc gacgggcgtg cgctgccgct
     5701 gcagctggcc ggtgaaggcg ctgccgtcgg cacccatcgc gtgctggtgc tgaacgaagc
     5761 ccgcagcagc tacacgttca cgggcatcga cagcgcaccg gtgccgtcgc tgctgcgcgg
     5821 attctcggcg cccgtgatcc tgggcgacga cctcaacgac accgatctgc tcgtgctgct
     5881 gaagcacgac accgacccct tcagccgctg ggaagcgggc cagcgtcttg cgctgaaccg
     5941 cctgctggct gccacgcagg ccggcgaggc gcaacccctg gacaacgact tcatcgaggc
     6001 gatgcgcgac atcctgcgtc accccggact cgatgccgcc ttcaaggaaa tggtgctgac
     6061 tccgccaagc gaaggctatg tctcggagaa gctgagcgtg gtccacccgc agcacatcca
     6121 caccgcccgg gaatcgatgc gctcccagct cgccgctgcg ctgcgcgaag actgggcctg
     6181 ggcgttcgag cagcatcagg tcggcggtgc ctattcgccc gacccggtgt cgtcgggcaa
     6241 gcgcgcgctg gcgaacctgt cgctggccat gctgtgccta gacgccgcgg ctcagggcga
     6301 tgcagtctgg ccgggccgcg cctaccagcg cttcaaggac gcggtcaaca tgaccgaccg
     6361 ccttggcgcg ctgtcggcgc tggtccattc gcatgccgaa ctggcgacgc ccgcgctgga
     6421 gcggttccac gcgatgttca agggcgaggc cctggtcatc gacaagtggt ttgcactgca
     6481 ggccaccgca ccggagatcg acggccgcgt gttcgcgcgt gcgcagctgc tgctgaagca
     6541 cccggatttc tcgatccgca atccgaaccg cgcgcgcagc ctgattgcgt cgctgtgcat
     6601 gatgaatccg gcagccttcc accgcacgga cgcggcgggc tatgtgttct gggccgaccg
     6661 ggtgctcgag ctcgacgccg cgaacccgca gctggccgcg cgcatggcgc gcatgctcga
     6721 ccggtggagc cgcctcgcgg agccgtaccg cagcgcggca cgcgaagcca tcagccgcgt
     6781 ggcggctaag tcggacctgt cgagcgacgt gcgggaaatc gtgtcgcggg cgctggccag
     6841 ctgaaggaga aatcatgagc aaacgagtca gcctgacgca gtacctggtc gaacagcagc
     6901 gcgagcacgg gcacatcccc ggccagctgc gcctgttgat cgaggtggtg gcgcgcgcct
     6961 gcaagcgcat cagcatcagc gtgaacaagg gcgccctcgg cgacgtgctg ggcagcgccg
     7021 agacggagaa cgtccagggc gagatgcaga agaagctcga catcatcgcc aacgaggtgc
     7081 tgatcgaggc gaacgaatgg ggcggccacc tcgcggcgat ggccagcgag gagatggaag
     7141 ggatctacgt cgtgcccaac cgctttcccc agggcgaata catgctgctg ttcgacccgc
     7201 tcgacggctc cagcaacatc gacgtcaacg tcagcatcgg cacgatcttc tcggtgctca
     7261 agcgcgagga ctccggccgc ccggtcagcg aagccgactt cctgcaaccc gggcgccagc
     7321 aggccgcggc cggttactgc gtgtacggac cgcagaccac cctggtgctg acagtcggcg
     7381 acggcgtcgc gatgttcacg ctcgatcgtg aacagggttc gtgggtgctg acacaggacg
     7441 gcctgcgcat ccccgaggac accaaggagt tcgccgccaa catgagcaac atgcgccact
     7501 gggcgcctcc gatgcggcga tacatcgacg aatgcctggc cggcaccgaa ggtgtgcgcg
     7561 gcaaggactt caacatgcgc tggatcgcca gcatggtggc cgacgtgcac cgcatcctga
     7621 ctcggggagg cgtgttcatc tacccgtggg acaagcgcga gcccggcaag gccggcaagc
     7681 tgcggctgat gtacgaggcc aatccgatgg gcttcctcgt cgaacaggcc ggcggcgccg
     7741 ccaccaacgg acaccagcgc atcctcgaca tccagcccga cgctctgcac cagcgcgtca
     7801 gcgtgatgat cgggtcgaag aacgaagtcg accgcctgac ccgataccac caggaagccg
     7861 actgaagacg caccgaaccc gacgtcccag ctataattcg aggcttcgcc ggtgtagctc
     7921 agttggtaga gcagctcatt cgtaatgaga aggtcgaggg ttcgactcct ttctccggca
     7981 ccagatcagt caggcaccca gagccccgtc caaaagacgg ggctttgctg tttctacggg
     8041 gccggcatgc aaactgctgc accgcagacg cgcatcgccc tcgagaccac gcggcacacg
     8101 cattgaactg cgtggcattt ccggagaccc ttcgcccatg aacgccacac gccactcaca
     8161 aagcgacgag cccggctcga gccattcgct cccgcttctg tacaacgtgg tttattgcag
     8221 ccgcgccaca gcggaagtgg acgatgccgc ggtggcgcgc atcctggaga ccgcccggca
     8281 gcgcaatccg gtgcatggca tcacaggctt gctggtcttc ggcggcggca ttttcttcca
     8341 gtggctggaa ggcccgcgcg acaacgtgac ccagctgatg acgaacctga aggcggatcc
     8401 gcgccacaag gacgtggtgg cgctcaccga gagcgaggag gcgcgcgagc gcctgttccc
     8461 cgattgggac atggagttcg tgaccaccga cgacatccgc gatgtgctga tcgacgcgat
     8521 gagcacgacc gaagacgcga agcacgcaga agccctcaag ctgcttctgg tgcacctgga
     8581 ttcgggcgag ttgagtggct tgaccacggc ctgatcggcc gcattttctg gtttcagggc
     8641 acctgctctg gccccgcagc ccgctggcca cgctccatgt cgacctttgc cagcagcagc
     8701 agtccgccga cgaagaaccc ggcggtcacg cccatcgcca ggcgctggtt gccctcggtg
     8761 agccaggtga cgagaccata ggtcagcagc cccagaaccg ctgaggcgcg ggtcgcgaag
     8821 gtccacagcc cgaagaactc ggcgcgccgg tcgccgggcg tgaaaagccc ggccatcgcg
     8881 cgccctgcag actggctgga tcccatgcac agacccgcga tcattgccgc gacccagaac
     8941 aaggcaggcc cacgggccag cacggccagc accgtcatca ggatccatcc cagcagggtg
     9001 accgacagcg cccgcttgtg accgatgcgg tcctgccagt agccgaaggc gaaggctcca
     9061 cccgccgcag caatgttgac gacgaagatc agcagcatcg tgtcggcctg cttgaagccc
     9121 agcgcctgct cggcatagac cgccgcaagc gcgaccacga cggagatccc cgcctgatag
     9181 caggcgccgc aagcgagcag ccacgagaag tcgcggtact gtcgcgcgtg cctccaggtg
     9241 tcggccaagc gagcgaaaga cgctccccag ccgcccgatt tcaacgacga agggaccttg
     9301 ccggcctgat cgcgcccggc gatgaactgc gggaccgccc tctcgcgcaa gagcatgaag
     9361 gtcgcgaccg aagccagcgc gtaagtggct gcggtgatca gcatcgtcac cggcacgaat
     9421 gaggtggcgg gctcgccgcg agcctgcgcc gacagcacgt aggccagacc cagccccagc
     9481 gccagcatgc ctcccaggta gccgaagctc cagccccagc cggacacgcg gcccatcgcc
     9541 tcggggcgag ccagttcagg caggaaggct gccgtcagcg attcgccgta cgaatacgcg
     9601 atgttggaaa cgacgaccgc gaggatcgcg agccccaggt cgcccggccc tgcacccgcc
     9661 agcgcggcgg tgccgagcac gcagaccacg gtgcagccgc cgagcaggcg cttcttggcc
     9721 gcccgcagat cggcataggc accgatcgcg ggcagggtca gcatcacgat caggctcgac
     9781 gccgcgatcg cgatcgtcca ggccagcgtg ccccaggcag ccccctgcgc gacgacgctg
     9841 acgaagtacg cgttgaacac ggctgtcagc acgacggtgg tgtagcccga gttggcgaag
     9901 tcgtacatcg cccagccgaa cacctcgcgc ttgcgcacgc catcgttgag cagccgctga
     9961 gagaacaggt ccacggacag gggctacgcg ccagccttcg atgcgatcac cgatcgtggt
    10021 cgccggtggg cgacaggctg gccagcacct tgtcgatgcg cttgccgtcg agatcgacga
    10081 cctcgaagcg ccagccttcc cactcgacgc tgtcggtggt ctgcggcagg cggccgagca
    10141 gcagcatgat catgccggcc agcgtgttgt agcgaccgcg tccctcctcg ggcaggtcct
    10201 tcagttccag gcggtccttc agttccggca ccggaatcag cccgtcgaag agccaggtgc
    10261 cgtcctcgcg ctgcaccgcc caggcgtcgt cgctcggggt ggtgaattcg ccggtgatgg
    10321 cttccagcac atcgcgcacg gtgatcatgc cctgcacctc gccgtactcg tcgaccacga
    10381 acacgagctg cgcgctcgac gcgcggaatt ggtccagcag ctccatgccc gacagggtct
    10441 cgggcacgaa caccgccggc tgcagacgct cggtgatcga cagcaccgag ccaccgatcg
    10501 cctgctgcag caggctctgc gccgacacca cgccgaccac gtcgtcgagc ccgccgcgac
    10561 aaaccgggta gcgcgaatgc tcgtccgcgg cgatgacctt cagcaccgca tcggcaggcg
    10621 ccaccgcgtc gagccagacg atctccgcac gcgggatcat catcgagccc acctgtcggt
    10681 cgtcgaggcg gaacacgttg cgcaccatct ggtgttcctg cgcctcgatc actccggcat
    10741 cgaggccttc ttccaggctg gcagcgatct cctcttcggt cacgctgcgg ctgggcccgc
    10801 cgcggatgcc catcaggcgc aaggtgctct cggtgcagaa cgacagcagc atcacgaacg
    10861 gccttgccga catcgacagc cagttcatcg gctgtgccac gagtcgcgcc acggactcgg
    10921 ggtacatctg acccagtcgc ttgggcacca gctcgccgaa gatgatggtc aggaaggtga
    10981 tcaccaccac caccatggcg gtcgcgagga tctccgcggc gttgtcgtgc atgtcgaacg
    11041 tgtgatgcag ccagagcgcg aaaggagccg aaaaggcggc atccccgacg atgccgttga
    11101 gcacgccgat cgatgtgatg ccgatctgca ccgtcgacag gaacttggtc ggatcgtcgt
    11161 gcagttccat cgcggccacg gcgcccgcat cgccggtttc caccatcacc tgaagccgcg
    11221 ccttgcggct ggccgtgagc gccatttcag acatggcgaa cagcgcattg agaaagatca
    11281 ggaagaccag taagacgacg tccatgcggg gcgtgagtga ggcgggtcgg ggcagcgtag
    11341 cagaatcgcc ggatggtcta tctcatcggc gacgtgcaag gctgctgcgg ggcgctgcag
    11401 cggctgctgg acaccatcgg cttctcgcct tcccgcgacc acgtgatcgt gctgggtgac
    11461 ttggtcaacc gcgggcccga cagcctgggc acgctgcgcc tgctgcgcga cctgggcgag
    11521 gcagccacct gcctgctcgg caaccacgac ctgcacctgc tggccgtggc ccacggcatc
    11581 cgcaaggccc atcgcagcga tacggtctcg gagatcctcg gcgcgccaga ccgcgatgcc
    11641 tggatcgatt ggcttcgcca tcgcaggatg gcgctgcacg agcacggctg gctgatggtg
    11701 cacgcgggcg tgccgccgcc ctggaccctg cagacgacgc tgcagctcgc ggccgaggtc
    11761 gagcagcagt tgcgcgggcc tgacctgcac gggttcctgg cggtgatgta cggcaaccag
    11821 cccgatcgat ggagcgacac gctggtcggc ccggaccggc tgcgctttgc cgtcaatgca
    11881 ctgacacgcg cacgcttcgt cgcggccgac ggcacgctgg atttcgaaac gaaggaaggt
    11941 gccggaggtg ccccgccagg tctggtgccc tggttcgagc atccgaagcg ccgcacggcc
    12001 ggggtgccca tggcattcgg ccactggtcg accctcggcc tgatccatcg ccccgacctg
    12061 ctggcactgg acaccggctg tgtctgggga ggcctcctga gcgcagcccg actcgttccg
    12121 ggcgggcgct ccgagatcat ccaggtcgaa tgcgaacagg cacaacgccc cggctgaacg
    12181 gcacgccgcg cggggccttg atgcgatgca cgcccctaca attactgcgt tggaagcgtg
    12241 gccgagcggt ttaaggctgc agtcttgaaa actgccgtgg gggtaacccc accgtgagtt
    12301 cgaatctcac cgcttccgcc acccttcccg acttccggcg cgatcaaggc gccgcgaagg
    12361 tggcgcccac cgcaagagcc tccgacatgc tcactctgca ggtgctggct gttccgctgc
    12421 aggccccgct ccagccggag aacaccatac ccgtggctgg cttggcagac agcgtgaccg
    12481 ccgtgccgct gttgaatgcc ttggtgcaag taccgccgca gctgatgccg gccggcgacg
    12541 acaccaccgt gccgctgccg gtgcgcgtga gggtcagcgg gtaggtgatg cgcacgaagg
    12601 tcgcgctcac gctgcgcgcg gcactcatcg tcacagtgca tgttccggtt ccgctgcaac
    12661 cgctgccgct ccagccgctc aggcgcatgc cgtcggctgg caccgcggtc agggggacgg
    12721 tggtgttgag gggctgcgaa ctcttgcaga cggtgccgca gtcgataccc gccggagcag
    12781 acgtgatgga gccggtgccc gtgcgcgcga cggtgagcgt gtagtgggtg cgcgtgaacg
    12841 tggcggccac cgcgcgcggc tccgacatcg tcacggtgca gggatcggtt ccggtgcatg
    12901 cgccggccca gccggagaag gccgattcgg ccaccggcaa cggcaccagc gtgaccgcgc
    12961 tgcccagcgg catcagcttg ctgcaggtgg taccgcagtt gatccccgca ggtgtggagg
    13021 tgaccgttcc cgtgctggtg ccattgcgcg tcaccgacag ggcgtagtgc gtcagggaga
    13081 acgtggcccc gaccgatgcg gcttcgttca tcgtgaccga gcaggtcagc gagctgccgc
    13141 tgcaggcgcc ggtccaaccc gcaaaggcag accccgccac cggcttggcc gtcagcttca
    13201 cgacggtcag gctgttgaac acgaagcggc aggtggcccc gcagttgatg ccggcaggag
    13261 ccgaggtgac gctgcctccg ccggtgccgt cgcgtgccag gtccagcgcg taggtgttgc
    13321 gtgtgaaggt ggcgatgacg gtgcgcgcgg cacccatcga gacttcgcat gcaccggtac
    13381 ccgagcaggc gccggcccat cccgtgaagc gcgatcccgc tgcgggcgtc ggggtcaggg
    13441 acacggttgt ctcgaccgtc accggaacag tgcagaccgt tccgcaattg atgcccgcag
    13501 gggacgatgc gacaccaccg gtgccggtgc ccgagcgcga cacggtcaat gtgttgtgcg
    13561 tgcgcacgaa tgtggccgtc acgcggctgg cctgggacag ggtgacgctg caggtgccgg
    13621 tgccgctgca ggcgccgccc cagccggaga aggctgagcc ggcggctggc ttcgcgctga
    13681 gcgtcaacag cgtgccatag gtgcgcgtga cgctgcaggt ggtgccgcag gacacgcccg
    13741 acggggtcga tgtcaccgtt ccggtgccat taccgtcctt gaccaccatc aacgagaacg
    13801 acagcagccc gaagttggcc gtcacgctgc gcgactggct catggccacg gtgcacgaca
    13861 gcgaagcgcc ggtgcaggca ccactccagc cgacaaactt cgagcccgtc gcgggcacgg
    13921 cagtcagcgt caccgaggta cccgcgttga gcaccttgct gcaggtgctg ccgcagtcga
    13981 ggccggcagc cacc
//
LOCUS       DAOORV010000096        13909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5372_length_13909_cov_6.834127, whole genome shotgun sequence.
ACCESSION   DAOORV010000096 DAOORV010000000
VERSION     DAOORV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_13909_cov_6.834127"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1275)
                     /locus_tag="PKV56_12665"
     CDS             complement(55..1275)
                     /locus_tag="PKV56_12665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOX68710.1"
                     /translation="MDLTPHFPIYMDYGATSPVDQRVVDAMIPWLREHFGNPASRSHA
                     WGWEAEAAVEKAREQVAGLVGADPREIVWTSGATESINLALKGAAQFYKTRGKHIITV
                     KTEHKAVLDTVRELERQGFDATYLEVEEDGLLDFEKLKAAIRPDTILVSVMFVNNEIG
                     VIQDIPAIGTLCRERGIILHVDAAQATGKVAIDLATLPVDLMSLASHKTYGPKGIGAL
                     YVRRKPRVRLEAQMHGGGHERGMRSGTLPTHQCVGMGTAFDIARQEMATESERIRALH
                     TKLVSGLTDIEQVFINGDMQHRVPHNLNISFNYVEGESLIMGVKGIAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRMTIGRFTTEAEIDYAVSTLKDRVAKLRELSPLWDMY
                     RDGIDISSIQWAAH"
     gene            complement(1347..1880)
                     /locus_tag="PKV56_12670"
     CDS             complement(1347..1880)
                     /locus_tag="PKV56_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HOX68711.1"
                     /translation="MRLTTKGRFAVTAMIDLALREHSGPVALAAISARQQISLSYLEQ
                     LFGRLRRHELVESTRGPGGGYSLGRKSEEITVADIILAVDEPLDATGCGGKENCMGED
                     GGRCMTHDLWASLNTKMIEHLNSISLKKLVEDQLAKGISVEEHPVKRAISTQPVVKPI
                     RITAPNSVFALGNAFSK"
     gene            complement(1991..2527)
                     /locus_tag="PKV56_12675"
     CDS             complement(1991..2527)
                     /locus_tag="PKV56_12675"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOX68712.1"
                     /translation="MVCMGNICRSPTAEGVLRQKLVAAGLADVVEVDSAGTHDWHAGK
                     PPDPRSVTHARNRGYDLSSLRSRPVTAADFERFDLILAMDWDNLALLQESCPALHQRK
                     LRRLSEFAVARASSVVPDPYQGGPDGFEHVLDLVEDACDGVVRFCPGRRMFNQAELPD
                     PMPTKSVGFRYTRQNCSG"
     gene            complement(2542..4653)
                     /gene="uvrB"
                     /locus_tag="PKV56_12680"
     CDS             complement(2542..4653)
                     /gene="uvrB"
                     /locus_tag="PKV56_12680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOX68713.1"
                     /translation="MADRVDTEPEVGADLPEVGRFVSYPGSPYQLFQPYAPAGDQPTA
                     ITQLVEGVLDGLSFQTLLGVTGSGKTFTMANVIAQLGRPAIVFAPNKTLAAQLYAEFR
                     EFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDV
                     VIVATVSAIYGIGAPEDYLEMRLIVRTGGKQSQRDLIAQLIRMQYTRNETDFSRGTFR
                     VRGDTIDIFPAEHSELAIRIELFDTEIESLSLFDPLTGRVRQKIPRFVVYPSSHYVTP
                     RDKVVNAIATIKDELRERLAELVGMGKLVEAQRLEQRTRFDLEMLQEVGHCKGIENYT
                     RHLSGAAPGQPPSTLVDYLPKDALMFLDESHVMIGQLGGMYNGDRARKTTLVEYGFRL
                     PSALDNRPLRFEEFETKMRQVVFVSATPAAYEQKTSGQVVEQVVRPTGLVDPMVEVRP
                     ATHQVDDVLQEIRIRVDLSERVLITTLTKRMAEQLTDYLSDNGVKVRYLHSDIETVER
                     VEILRDLRLGVFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAA
                     RNVNGRAILYADKITESMRRAMGETERRRNKQIAHNLEMGITPRTINKKIKELIDGVY
                     AEKSGKDDLKSAKGAAEVEMLSEKDLGKRIKQLEKQMLEHARNLEFEKAARVRDQLAL
                     LREQAFGAPGSDNITALVAKP"
     gene            4789..6000
                     /locus_tag="PKV56_12685"
     CDS             4789..6000
                     /locus_tag="PKV56_12685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOX68714.1"
                     /translation="MASLFAAVDMAPRDPILGLNEQFNADPNPAKVNLGVGVYFDESG
                     KLPLLKCVSAAEKLIFESSKPRGYLPIDGIAAYDKAVQGLVFGADSAAVKDGRVATVQ
                     ALGGTGGLKIGADFLQHLATRAGTSPKVLISDPSWENHRALFANAGFTVETYPYYDAD
                     KRGINFDGMLGALNAAAAGTIIVLHACCHNPTGYDITPAQWAQVVAAVKSRGLVAFLD
                     MAYQGFGDGIAEDGAVIAQFLDAGLDFFVSTSFSKSFSLYGERVGALSVVCASKEEAV
                     RVLSQLKIVIRTNYSNPPTHGAQIVATVLTTPALRAMWEEELAGMRVRIKEMRKLLQD
                     RLVASGTKQDFSFITRQKGMFSYTGLAKPQMERLRNEFGIYGVDSGRICVAALNHGNI
                     DAVVKAIGLVT"
     gene            6350..7750
                     /gene="phaZ"
                     /locus_tag="PKV56_12690"
     CDS             6350..7750
                     /gene="phaZ"
                     /locus_tag="PKV56_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOX68715.1"
                     /translation="MSLYQFYEAQRALLNPLAEFSSASSKLYSHPLSPFTHTPMAPRV
                     SAGLNLMHRLSKAYEKPEFNIHTVKVDDVDVAVQQQVTIEKPFCRLLRFKRFTDDLPM
                     LKTMKDQPKVLVVAPLSGHHSTLLRETVRELLQHHKVYITDWTDARMVPAEMGPFHLD
                     DYVDYIQEFIRHIGPDVNVISVCQPTVPVLAAISLMASRGEKTPRTMTMMGGPIDARL
                     SPTAVNNLAMNKSLEWFEHNVIFRVPTNYPGSQRPVYPGFLQHTGFIAMNPSNHLRSH
                     YDYYMDLMRGEDENADFHRNFYDEYNAVLDMPAEYYLDTIRVVFQDFALVNGTWEVHG
                     EPVRPQDIKTTALLTVEGEKDDISGAGQTRAAHGLCTGIDRKLQFHYDAIGAGHYGIF
                     SGRRWRENVYPVVRDFIASHQVRQTAAPAKVRAKPVAKPAAKATAAEPALKLVPTSPT
                     ARKSSPRSSPKSRLAK"
     gene            7755..8492
                     /gene="rsxB"
                     /locus_tag="PKV56_12695"
     CDS             7755..8492
                     /gene="rsxB"
                     /locus_tag="PKV56_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOX68716.1"
                     /translation="MVSKPAVRHPDAAAAGSTHATCGLIERLDRALPQTQCTRCGYPD
                     CRSYAQAIGDASAGINRCPPGGVEGIARLAAITGESILPLDASRGVEGPRHLAVIDEA
                     SCIGCTLCLPACPVDCIVGGPKAMHTVIDALCTGCELCLPACPVDCITLVNVSGDLTG
                     WRAWSQPLADEARQRYEFHRFRLEREHTERNERLEARALSLRPLEVAHQPTTPVATAS
                     EAEPAPDDRKRAVIEAAMARARAARRT"
     gene            8489..10399
                     /locus_tag="PKV56_12700"
     CDS             8489..10399
                     /locus_tag="PKV56_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX68717.1"
                     /translation="MSEPKRALRVLSVIPPMTQLNTPYPSTAYLTGFMRSRGIDAVQE
                     DLALALVLELFSVDGLLSVRHDIEASPERQRTPRTAAFLDAFERYHSTISPTLAFLQG
                     RDSTLAHRICSRAFLPEGPRFESLDVYIDEDGGDPLAWAFGALGVQDRARHIATLYLN
                     DIADVLRDAIDPRFEFVRYAESLARSQPTFDPLAEALSAAPNLIDCTLAALTEDALAR
                     HRPDVVLVSVPFPGAVYAAFRIAQTVKAHDPAIVTVLGGGYVNTELRELKEQRVFDYF
                     DHITLDAGERPLLSLLEHLQGQRSVQRLVRTYRRDSEGAVHYVNWIEADIAFAEVGTP
                     TWDGLPLDRYLSLLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLDYISRYEG
                     ASAATLVDRIETIVAETGQTGFHFVDEAAPPKALKTLSAELLHRQLAISWWGNIRFEK
                     SFTPELCQQLADSGCIAISGGLEVASDRLLNLMKKGVSVEQVARVTKAFSDAGVLVHA
                     YLMYGFPTQTVQDTVDALEYVRQLFEQGCIQSGFFHRFACTVHSPVGQKPEDYGITLQ
                     PLPPITFATNDVDFIDPTGVDHDALGVALKKALYNYMHGIGLDEDVRSWFGPRTPRSR
                     VPRDRIARALAA"
     gene            10414..11040
                     /gene="nth"
                     /locus_tag="PKV56_12705"
     CDS             10414..11040
                     /gene="nth"
                     /locus_tag="PKV56_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOX68718.1"
                     /translation="MKKTDIAPFFAVLQVANPRPQTELEFTNVFELLTAVLLSAQATD
                     VGVNKATRRLFAVANSPQKMLALGLPALTEHIRTIGLYRNKAKNLYETCRLLVERHGG
                     EVPRTREELEALPGVGRKTANVVLNVAFGEPTMAVDTHIFRVGNRTGLAPGKNPLQVE
                     LKLLERIPPEYLVDAHHWLILHGRYVCTARGPKCGECAVSAYCDSRLR"
     gene            11149..>13909
                     /gene="prsT"
                     /locus_tag="PKV56_12710"
     CDS             11149..>13909
                     /gene="prsT"
                     /locus_tag="PKV56_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HOX68719.1"
                     /translation="MKPHSSLTLCAKTLLTALLVASTSPGFAAIDTKAGRYYEDALAR
                     FNRKDAPGAIIQLKNALQIDKSMLPVHVLLGKALLINGEAIAAEVAFNEALRLGVNRA
                     EVVVPLAKSVMSQGRLQEVVDKPLFATAGLPVATQVQMLLLRASATVDLGGSNNAMKA
                     IEEARALDPGSPDSWLAEVPMRIRAGRFGEAQMAVDRALLLAPSSAEAFYLRGTVAHI
                     QGDSAGALASYAKALLIQPSHTEALVSRAGLLMDQQRVAEAATDVAELLKSSPNDPRG
                     HYLGSLISERQGRPAEAKAALHKITALLDPVPIDSLRYRPQVLMLGGLAHYGLNEREK
                     AKPYLEAALRAQPGSGVAKLLAQIYLSDKNTDRAISALDSYLKVHPGDSQALLLLASC
                     HIAQGRHARATQLMQDAMRGQDLPEFQTMLGMSLVGSGNFGDALAALESAFRKDPKQF
                     QAGVALATLYLQGGQAARSVTIAESLAKQRPGSPGVQNLLGRARAAAGDRAGAKAAFE
                     SAAKLDASFSEPQVNLARLDTDAKAFDAAATRLTSVLAKDQKNLDAINEMARLLERRG
                     QPAEAQRWLEKAIDYSGPNDLQPNLALVEFQLRNNNPAAAQLAAKGLTGKAPEALPVL
                     LASARVSLANGDATAARTNLSRATTQATRNPPVLLQIALLQSTAGDLAGAAYSLDKAL
                     SERPDFPPAKALLAEVEMRRGELDKAEAHVRQIIASQPKSGVGYGLMGDLASARGQRP
                     AAIEAYRRAHQIEQTTASLMRLYGILAQEDGPGAVQLADQWVKSHPQDTVARRALADG
                     HARAGNLTAARAAYEALVKMAPGDAESLNNLANVLLLTKDPGALKIAEQALALDPKAP
                     HIIGTVGWAAFKAGQTDRALQFLRDARLRDPDNRDTRYFLGAVLASSGRPTEAREELE
                     AALKGD"
BASE COUNT         2518 a         4627 c         4349 g         2415 t
ORIGIN      
        1 tcgtagtggt cgatgacctt gtcgctgtat gccatgtcag tcctccaaac agtcttaatg
       61 tgcagcccac tggatgctgc tgatgtcgat gccgtcgcgg tacatgtccc acagcggcga
      121 gagctcgcgc agtttcgcca cgcggtcctt caaggtgctg acggcatagt cgatctcggc
      181 ctcggtggtg aagcggccga tggtcatgcg caggctgctg tgggccagct cgtcgctgcg
      241 gcccagggcg cgcagcacat agctgggctc caggctggcc gaggtgcagg ccgagccgga
      301 gctgaccgcg atgcccttca cgcccatgat cagcgactcg ccctcgacat agttgaacga
      361 gatgttcagg ttgtgcggca cgcggtgctg catgtcgccg ttgatgaaca cctgctcgat
      421 gtcggtgagg ccgctgacca gcttggtgtg cagcgcgcgg atgcgctcac tctcggtcgc
      481 catctcctgg cgggcgatgt cgaaggccgt gcccatgccg acgcactggt gcgtgggcag
      541 cgtgcccgag cgcatgccgc gctcatggcc gccaccgtgc atctgggctt ccagccgcac
      601 gcggggcttg cgccgcacgt acagcgcgcc gatgcccttg ggcccatagg tcttgtgcga
      661 ggccaggctc atcagatcca ccggcagcgt ggccaggtcg atcgccacct tgccggtggc
      721 ctgggcggcg tcgacgtgga ggatgatgcc gcgctcgcgg cacagggtgc cgatggccgg
      781 aatgtcctgg atcacgccga tctcgttgtt gacgaacatc accgacacca ggatggtgtc
      841 ggggcggatc gccgccttca gcttctcgaa atcgagcaac ccgtcttcct ccacctcgag
      901 gtaggtggcg tcgaagccct ggcgctcgag ttctcgcacc gtgtccagaa ccgccttgtg
      961 ctcggtcttc acggtgatga tgtgcttgcc gcgcgtcttg tagaactgcg ctgcaccctt
     1021 cagggcgagg ttgatcgatt cggtggctcc cgacgtccag acgatctccc gaggatcggc
     1081 acccaccagt ccagcgacct gctcgcgggc tttctcgacc gccgcttcgg cttcccatcc
     1141 ccaggcgtgg ctgcgtgagg ccggattgcc gaaatgctcg cgcaaccaag ggatcatggc
     1201 gtcgacgacg cgctggtcca ccgggctggt ggcgccgtag tccatgtaga tcgggaagtg
     1261 tggcgtcaga tccatggttc gatttctgct cggggatggc ggctgcgggg gcgcccatgc
     1321 gccagcttca ccacagcggt tcaacgttat ttcgagaagg cgttgccgag ggcaaagacg
     1381 gagttcggtg cagtgatgcg aatgggcttc acgacgggct gcgtcgagat cgcgcgcttg
     1441 accggatgct cctcgaccga aattcccttg gccaattgat cctcgaccag cttcttcagc
     1501 gagatcgaat tcaggtgctc gatcatcttg gtgttgaggc tggcccacag gtcgtgggtc
     1561 atgcagcggc cgccgtcctc gcccatgcag ttttccttgc cgccgcaacc cgtggcatcc
     1621 agcggctcgt cgacggccag gatgatgtcg gcgacggtga tttcctccga cttgcggccc
     1681 agcgaatagc cgccgcctgg tccgcgcgtg ctctcgacca gttcgtgacg gcgcagccga
     1741 ccgaacagct gctccaggta cgacagggaa atctgctggc gggcgctgat ggcagcgagc
     1801 gccaccggac ccgaatgttc acgcagcgcc agatcgatca tggcggtgac ggcaaaacga
     1861 cccttggtgg taagacgcat ggaactctcc tctcgcccct tggggcatca cgaacaagac
     1921 ggcctggttg gcaacgtccg cctgccggca atctgagggc tcgggaaacg cctcttttgg
     1981 aggccgatcc ttaacctgag cagttttgtc gagtatagcg gaacccaact gatttggtcg
     2041 gcattgggtc aggaagttcc gcctgattga acattcttcg tcctggacaa aacctgacaa
     2101 ccccatcaca agcgtcctcc accaggtcga gcacgtgttc gaagccatcc gggccccctt
     2161 ggtacggatc cggcaccacc gagctggcgc gggccaccgc gaactcggag aggcggcgca
     2221 gcttccgctg atggagcgca gggcacgatt cctggagcag cgcgaggttg tcccagtcca
     2281 tcgccaggat cagatcgaag cgctcgaaat ctgcggcagt gaccggccgt gaccgcagac
     2341 tcgacaggtc gtagccgcga ttgcgcgcgt gtgtcaccga ccgcgggtcg ggcggcttgc
     2401 ccgcatgcca gtcgtgcgtg cccgccgaat cgacctcgac gacatcggcc aggcccgctg
     2461 cgaccagctt ttgtcgaagc acgccttcgg cggtgggcga tcggcagatg ttgcccatgc
     2521 acaccatcag caccgacttc ctcacggctt ggccaccagc gcggtgatgt tgtcgctgcc
     2581 aggcgctccg aaggcctgct cgcgcagcaa ggccaattga tcacgcaccc gggccgcctt
     2641 ctcgaactcg aggttgcgcg catgttcgag catctgcttc tcgagctgct tgatgcgctt
     2701 gccgaggtcc ttctccgaca gcatctcgac ctcggcagcg cccttggccg atttcagatc
     2761 gtccttgccg gacttctcgg catagacacc gtcgatcagc tccttgatct tcttgttgat
     2821 cgtgcgaggc gtgatcccca tctcgaggtt gtgcgcgatc tgcttgttgc gacggcgttc
     2881 ggtctcaccc atcgcgcgcc gcatcgattc ggtgatcttg tcggcgtaaa ggatcgctcg
     2941 gccattgacg ttgcgcgctg cgcggccgat ggtctggatc aggctgcgct ctgcacgcag
     3001 gaatccttcc ttgtcggcgt cgaggatcgc caccagcgac acctcgggaa tatcgagccc
     3061 ctcgcgcagc aggttgatgc cgaccagcac gtcgaaaacg cccagcctga ggtcgcgcag
     3121 gatctcgaca cgctcgaccg tctcgatgtc gctgtgcagg tagcgcacct tgacgccgtt
     3181 gtcgctgagg taatcggtca actgctcggc catgcgcttg gtcagcgtgg tgatcagcac
     3241 gcgctcggac aggtcgacgc ggatgcggat ctcctgcagc acatcgtcga cctgatgcgt
     3301 cgccggccga acctcgacca tcgggtcgac caggccagtg ggccgcacga cctgctcgac
     3361 cacctgaccc gaggttttct gctcgtaggc ggccggcgtg gccgaaacga acaccacctg
     3421 gcgcatcttg gtctcgaact cctcgaaacg caggggccgg ttgtcgaggg cgctcggcag
     3481 gcggaagccg tactcgacca gcgtggtctt gcgcgcgcgg tcgccgttgt acatgccgcc
     3541 gagctgcccg atcatcacgt gcgactcgtc caggaacatc agcgcatcct tcggcaggta
     3601 gtcgaccagc gtcgacggcg gctgtcccgg cgcagcgccc gacaggtggc gggtgtagtt
     3661 ctcgatgccc ttgcagtgac cgacttcctg cagcatttcc agatcgaagc gggtgcgctg
     3721 ctccagccgc tgcgcctcga ccagcttgcc catgccgacc agctcggcca ggcgctcgcg
     3781 caactcgtcc ttgatggtcg caatcgcgtt gaccaccttg tcgcgcggcg tcacgtaatg
     3841 gctcgaggga tagaccacga accgcggaat cttctggcgc acgcgtccgg tcagcggatc
     3901 gaaaagcgac agggactcga tctcggtgtc aaacagttcg atgcgtatcg cgagctcgga
     3961 atgttcggcc gggaagatgt cgatcgtgtc gccgcgaacg cggaaggtgc cgcgcgagaa
     4021 atcggtctcg ttgcgcgtgt actgcatgcg gatcagctgc gcgatcaggt cgcgctggct
     4081 ctgcttgccg ccggtgcgaa cgatcagccg catctcgagg taatcctcgg gtgcaccgat
     4141 gccatagatc gcgctgacgg tagcgacgat caccacgtcg cgccgctcaa gcagactctt
     4201 ggtggccgaa agccgcatct gctcgatgtg ctcgttgatc gagctgtcct tctcgatgaa
     4261 caggtcgcgc tgcggcacgt aggcctcggg ctggtagtag tcgtagtagc tgacgaaata
     4321 ctccaccgcg ttcttcggaa agaattcgcg gaactcggca tacagctgag ccgccagcgt
     4381 cttgttgggg gcgaacacga tcgccgggcg gccgagctgc gcgatcacgt tggccatcgt
     4441 gaaggtcttg cccgagcccg tgaccccaag cagcgtctgg aagctcaggc cgtcgagcac
     4501 gccctcgacc agctgcgtga tggcggtggg ctggtcgcct gctggcgcat agggctgaaa
     4561 cagctgatat ggcgagccgg gataactgac gaaccgacca acttcgggca aatctgcacc
     4621 aacttcgggc tcggtatcaa cgcgatcggc catggcggat aaaatctcag ggttaactcg
     4681 cgttgcggcg ggacgggcca acttcaaagg gtagcccaaa tcacccgccg ccgcccacca
     4741 ccggcgcatc ccgcgccttg attcagctgc ccatcacaag gacttcccat ggcatcgctc
     4801 tttgccgccg tcgacatggc gccccgcgac ccgatcctcg gcctgaacga acagttcaac
     4861 gctgatccga acccagccaa ggtgaacctc ggcgtcggtg tctatttcga cgaatccgga
     4921 aagctgccat tgctgaagtg cgtttcggcg gcggagaagc tgatattcga gtcctcgaag
     4981 ccgcgcggct acctgccgat cgacggcatt gcagcctatg acaaggcagt gcagggactg
     5041 gtgttcggtg ccgacagcgc agcggtcaag gacggtcgcg ttgccacggt gcaggcactc
     5101 ggcggcaccg gtggcctgaa gatcggagca gatttcctcc agcatctggc cacccgcgcc
     5161 ggcacctcgc cgaaggtcct gatcagcgac ccgagctggg aaaaccatcg agcgctgttc
     5221 gccaacgccg gcttcaccgt cgagacctat ccgtactacg acgccgacaa gcgcggcatc
     5281 aacttcgacg gcatgctggg cgcgctgaac gcagccgcag caggcaccat catcgtgctg
     5341 cacgcctgct gccacaaccc gaccggctac gacatcaccc ccgcgcaatg ggcgcaggtg
     5401 gtggctgcgg tcaagtctcg cggtctcgtg gctttcctcg acatggccta ccagggcttc
     5461 ggtgatggca tcgccgagga cggtgcggtg atcgcccagt tcctcgacgc cgggctcgat
     5521 ttcttcgtgt ccacgtcatt ctcgaagagc ttctcgcttt acggcgaacg cgtcggcgcc
     5581 ctgagtgtgg tttgcgccag caaggaagaa gccgtgcgcg tgctgagcca gctcaagatc
     5641 gtgatccgca ccaactattc gaacccgccg acccacggcg cgcagatcgt cgccacggtg
     5701 ctgaccaccc cggccttgcg cgcgatgtgg gaagaggaac tggccggcat gcgcgtgcgc
     5761 atcaaggaaa tgcgcaagct gctgcaggac cggctggtgg cgtcgggaac gaagcaggat
     5821 ttcagcttca tcacccgcca gaaagggatg ttcagctaca ccggcctggc caagccgcag
     5881 atggaacgcc tgcgcaacga gttcggcatc tacggcgtgg actccgggcg catctgcgtc
     5941 gcggcgctga atcacggcaa catcgatgcg gtcgtcaagg cgatcggtct ggtgacttga
     6001 tccctcatcg atccggcatc cagaggcccg gcactgccgg gctttttcat ttgtgctggc
     6061 cggagaccaa tctgcaactt cgacggtggg aacggattga gccaggacgc gaagagctcc
     6121 atgccgagcg aattcgccag gttgacatcc tggcgcggca gctttctgcc gagaaaccgc
     6181 gggttcgact gtggtcttag ggcgcacaac ctacaatgct gcagtgcaac attcatactc
     6241 accacctgcc cgctgttatt ccggatggct gactggtgtt ctcggcgact agcattcgca
     6301 acccccgcgc cgcaccagcg aagccacccg tccgaacgag gatttctcca tgtcgcttta
     6361 ccagttctac gaagcccaac gggcccttct gaacccattg gccgagttct cgagcgcgtc
     6421 gtcgaagctg tacagccacc ccctgtcgcc attcacgcac accccgatgg caccgcgcgt
     6481 gtcggccggg ctgaacctga tgcaccgact gtcgaaggcc tacgagaagc ctgaattcaa
     6541 catccacacg gtcaaggtcg acgacgtcga tgtcgccgtg cagcagcagg tcacgatcga
     6601 gaagcctttc tgccggctgc tgcgcttcaa gcgcttcacc gacgacctgc cgatgctcaa
     6661 gaccatgaag gaccagccca aggtcctggt cgtcgccccg ctctcgggcc accactcgac
     6721 cttgttgcgc gaaaccgtgc gcgagctgct gcagcaccac aaggtctaca tcaccgactg
     6781 gaccgacgcc cgcatggtgc cggccgagat gggcccgttc cacctggacg actacgtcga
     6841 ttacatccag gagttcatcc gtcacatcgg acccgacgtc aacgtgatct ctgtgtgcca
     6901 gccaacggtt cccgtgctgg ccgccatctc gctgatggcg agccggggcg agaagacccc
     6961 gcgcacgatg acgatgatgg gcggcccgat cgacgcacgc ctgtcgccga cggcagtcaa
     7021 caacctggcg atgaacaaga gcctggagtg gttcgagcac aacgtgatct tccgcgtgcc
     7081 aaccaactac cctggcagcc agcgtcccgt gtacccgggc ttcctgcagc acaccggatt
     7141 catcgcgatg aatccgagca accacctgcg ttcgcattac gactactaca tggacctgat
     7201 gcgcggagag gacgagaacg ccgacttcca tcgcaacttc tatgacgagt acaacgcggt
     7261 gctcgacatg ccggccgagt actacctcga cacgatccgt gtcgtcttcc aggactttgc
     7321 gctggtcaac ggcacctggg aagttcacgg cgaacccgtt cgcccgcagg acatcaagac
     7381 gaccgcgctg ctgaccgtgg aaggcgaaaa agacgacatc tccggcgccg ggcagacgcg
     7441 cgcggcccat ggactgtgca ccggcatcga ccgcaagctg cagttccact acgacgcgat
     7501 cggagccggc cactacggca tcttctccgg ccgccggtgg cgcgagaacg tgtaccccgt
     7561 cgtgcgcgac ttcattgcat cgcatcaggt tcgccagacc gccgcaccgg caaaggtgcg
     7621 ggccaaaccg gtagcgaagc ccgcggccaa ggcgaccgca gcagagcctg ccctgaaact
     7681 ggtgccgact tcgccaaccg cacgcaaatc gtcacccagg tcatctccga aaagccgact
     7741 ggccaagtaa agcgatggtg tcgaagccgg ccgttcggca cccggatgcc gcagcggcgg
     7801 ggtcgactca cgccacttgc ggcctgatcg aacggctgga ccgcgcgctg ccgcagacgc
     7861 agtgcacccg ttgcggctac cccgattgcc gcagctatgc gcaggcgatc ggcgacgcaa
     7921 gcgccggcat caaccggtgc cctcccggag gcgtcgaggg cattgcgcgc ctggcggcga
     7981 tcaccgggga atcgatcctg ccgctggatg catctcgcgg agtcgaggga ccacgccatc
     8041 tcgcggtgat cgacgaggcc agctgcatcg gctgcacgtt gtgcctgcca gcctgccccg
     8101 tcgattgcat cgtcgggggg ccgaaggcga tgcacaccgt catcgatgca ctgtgcaccg
     8161 gatgcgagct gtgcttgcca gcctgcccgg tcgactgcat cacgctggtc aacgtgagcg
     8221 gtgatctcac cggctggcgt gcctggagcc agccgctggc cgacgaagcg cggcagcgat
     8281 acgaattcca tcgattccgg cttgaacgcg agcacaccga gcgcaatgag cgcctcgaag
     8341 cacgtgcgct gtcgctgcga ccactcgagg tcgcgcatca gccgaccact ccggtggcga
     8401 cggcatccga ggcggagccc gcgcctgatg accgcaagcg tgcggtcatc gaggcggcga
     8461 tggctcgcgc ccgagctgcc cgcaggacat gagcgagccc aaacgcgcgc tgcgggtgct
     8521 gtcggtcatc ccgccgatga cgcagctcaa cacgccctat ccatccaccg cctacctgac
     8581 cggcttcatg cgctcacgcg gcatcgatgc ggtgcaggaa gacctggcgc tggcgctggt
     8641 ccttgagctt ttctccgttg acggattgct gtcggtgcgc cacgacatcg aagcatcacc
     8701 cgaacggcaa cgcacgccgc gcaccgcggc atttctcgac gcattcgagc gttatcactc
     8761 gacgatctcg ccgacgctgg cattcctgca ggggcgcgac tccacgctgg cacaccggat
     8821 ctgcagccgc gcctttcttc ccgagggccc gcgattcgag tcgcttgacg tctacatcga
     8881 cgaagacggc ggcgacccgc tggcctgggc gttcggcgcg ctgggcgtgc aggatcgagc
     8941 acgacacatc gccacgctct acctgaacga catcgccgac gtgctgcgcg atgccatcga
     9001 cccccgcttc gagttcgtgc gctacgcaga gtctcttgcg cgcagccagc cgactttcga
     9061 cccgctggcc gaggcactgt ccgccgcgcc caacctgatc gactgcaccc tggccgcgct
     9121 aacagaggac gctctggccc gccacaggcc agatgttgtt ctggtgtcgg tgcccttccc
     9181 cggtgcggtg tacgcggcgt tccgcatcgc gcagacggtg aaggctcacg atccggcgat
     9241 cgtcacggtg ctcggtggcg gctacgtcaa caccgaactc agggagttga aagagcagcg
     9301 tgtcttcgat tatttcgatc acatcacgct cgatgccggc gagcgcccgc tgctgtcact
     9361 gctcgaacac ctgcagggcc agcgctcggt acagcggctg gtgcgcacct atcgacgtga
     9421 cagcgaaggt gccgtgcatt acgtcaactg gatcgaagcg gacatcgcct ttgccgaagt
     9481 gggtacgccg acctgggacg gtctgccgct ggatcgctac ctgtcgctgc tggacatgct
     9541 gaacccgatg caccgcctgt ggtccgacgg gcgctggaac aagctcaccg tggcgcacgg
     9601 ctgctactgg aagaagtgca gcttctgcga cgtcagcctc gactacatct cgcgctatga
     9661 aggcgcatcg gccgccacac tcgtcgatcg gatcgagacc atcgtcgccg agaccggaca
     9721 gaccggcttc cacttcgtcg acgaggccgc gccaccgaag gcactgaaaa cgctctcggc
     9781 cgagctgctg catcggcagt tggccatctc ctggtggggc aacatccggt tcgagaagtc
     9841 gttcacaccc gagttgtgcc agcaactcgc agacagcggc tgcatcgcga tttcgggcgg
     9901 gctcgaggtc gcttcggacc gactgctgaa cctgatgaag aagggcgtct ccgtggagca
     9961 ggtcgcacgc gtgaccaagg ccttcagcga cgccggcgtg ctggtccatg cctacctgat
    10021 gtacggcttt ccgacgcaga cggtgcagga caccgtcgac gcactggagt acgtgaggca
    10081 gctgttcgaa cagggctgca tccagtcggg cttcttccac cgctttgcct gcaccgtgca
    10141 ttcgccggtc ggacagaagc ccgaggacta cggcatcacc ctgcagccgc tgccgccgat
    10201 cacattcgcg accaacgacg tcgacttcat cgacccgacc ggtgtcgatc acgatgcgct
    10261 aggcgttgcg ctgaagaagg cgctctacaa ctacatgcac ggcatcggcc tcgacgaaga
    10321 tgttcgcagc tggttcggcc cccgaacgcc acgcagccgg gtgccgcgcg accggatcgc
    10381 tcgtgctctc gcggcctgac tggacacgcc acgatgaaga agaccgacat cgccccgttc
    10441 tttgccgtgc tgcaggtcgc gaaccccaga ccgcagaccg agctcgagtt caccaacgtg
    10501 ttcgagctgc tgaccgcggt gctgctgtcg gcgcaggcaa ccgacgtcgg cgtcaacaag
    10561 gcgacgcgga ggctgtttgc agtggcgaac tcgccgcaga agatgctcgc actgggactg
    10621 ccggctctca ccgagcacat caggacgatc ggcctgtacc gcaacaaggc gaagaacctg
    10681 tacgagacct gtcgcctgct cgtcgagcgg catggcggcg aagtgcctcg caccagggaa
    10741 gaactggagg cgctgcccgg ggtcggccgc aagacggcca acgtggtgct gaacgtcgcc
    10801 ttcggcgagc cgacgatggc cgtcgacacc cacatcttcc gcgtaggaaa ccgcacgggc
    10861 ctggcgccag gaaaaaaccc gctgcaggtc gagctgaagc tgctcgagcg cattccaccc
    10921 gaatacctgg tcgacgcgca tcactggctg atcctgcatg gccgctacgt ctgcacggcg
    10981 cgcggcccga aatgcggcga atgcgcggta tcggcctatt gcgattcgcg tcttcgctga
    11041 gtgccgaccc ctcgttcgcg ggatgcagtc ggtaacattt cacacactgg cgtgggcagc
    11101 gcgccgcacg cccgaatggc acgacatcgc gtcactacca tccgccgcgt gaaaccccat
    11161 tcctcgctca ccctctgcgc caagaccctg ctgacggccc tgctggttgc ctcgacctcg
    11221 ccgggttttg cagccatcga caccaaggcc ggccgctact acgaagacgc cctcgcgcgc
    11281 ttcaacagga aagacgctcc cggggcgatc atccagttga agaatgcgct gcaaatcgac
    11341 aagagcatgc ttccggtgca tgtgctgctg ggcaaggcac tgctgatcaa cggcgaggcc
    11401 atcgcagccg aggtggcctt caatgaagcg cttcgcctgg gagtgaaccg ggccgaagtc
    11461 gttgtgccgc tggccaagtc ggtcatgtcc cagggtcggt tgcaggaggt ggtggacaag
    11521 cccctgttcg caaccgccgg tctgcctgtc gccacgcagg ttcagatgct gcttctgaga
    11581 gcttcggcta ccgtcgacct gggtgggtcg aacaacgcca tgaaggcgat cgaggaagcc
    11641 cgagcgctcg acccgggcag ccccgactcg tggctggctg aggtcccgat gcgcatccgc
    11701 gctggcaggt tcggcgaagc ccagatggcg gtcgatcgcg cgctgctgct ggcaccgagt
    11761 tctgcagagg ctttctacct gcgcggaacg gttgcgcaca tccagggaga ttcggctgga
    11821 gcattggcga gttatgcgaa ggcactgctg atccagcctt cgcataccga agccctggtt
    11881 tcgagggccg gcctcctgat ggatcagcag cgagtggcag aggccgccac cgacgtggcc
    11941 gaactgctga aaagctctcc taacgacccg cgcggccact atctcgggtc gctgatctcg
    12001 gagcgtcagg gacgccctgc cgaagccaag gcagcactgc acaagatcac ggcactgctt
    12061 gacccggtgc ccatagactc cctgcgctat cgaccacagg tcctgatgct gggcgggctc
    12121 gcccactacg ggctgaacga gcgggagaag gccaaaccct acctcgaggc tgcgctgcga
    12181 gcccagccgg gcagcggggt ggccaagctg ctcgcgcaga tctacctgtc cgacaagaac
    12241 acggatcggg cgatttctgc gctggattcg tacctgaagg tgcaccccgg tgactcgcag
    12301 gccctgctgc tgcttgcgtc atgtcacatc gcgcaaggcc gacacgcacg cgccacgcaa
    12361 ctgatgcagg acgccatgcg cgggcaggat ctacccgagt tccagaccat gcttggcatg
    12421 agcctggtgg gaagcggcaa cttcggggat gccctggccg cactcgagag tgccttccgc
    12481 aaggatccca agcagtttca ggcgggtgtg gccctggcca ccctctatct gcagggggga
    12541 caggcagcac gctccgtgac gattgccgag agcctcgcca agcagagacc cggcagcccg
    12601 ggcgtccaga atctgctggg cagggcacgc gctgccgcgg gcgatcgtgc aggagccaag
    12661 gcggctttcg aaagcgccgc aaagctcgac gcttctttct cggaacccca ggtgaacctg
    12721 gctcggctgg acaccgatgc aaaggctttc gacgcagcag caacgcggtt gacctctgtg
    12781 ttggccaaag accagaagaa cctcgatgcc atcaacgaga tggcccggct tctagagcgg
    12841 cgaggccagc cagccgaagc ccagcgctgg ctggagaagg cgatcgatta cagcgggccg
    12901 aacgacctgc aaccgaatct ggccctggtc gagttccaac tgcgaaacaa caaccccgcc
    12961 gcggcccagt tggctgccaa gggcctgacc ggcaaggccc ccgaggcact gcccgtcctg
    13021 ctcgcatcgg cacgcgtcag cctggccaac ggcgacgcca cggccgcacg aaccaacctg
    13081 agccgagcca ccacccaggc cacccgcaac ccgccggtgc ttctgcagat cgccctgctt
    13141 caatcgacag ccggtgacct ggcaggagcg gcctacagcc tggacaaggc cttgtccgaa
    13201 cgcccggatt tcccgcctgc gaaggccttg ctggccgagg tcgagatgcg ccgaggcgaa
    13261 ctcgacaagg ccgaggcaca cgtgcgccag atcatcgcca gccagccgaa atccggggtc
    13321 ggctatggcc ttatgggtga tctggcctcc gcgcgcggcc aacgccccgc ggccatcgaa
    13381 gcctatcgtc gtgcccacca gatcgaacag acaacggcca gcctgatgcg cctgtacggc
    13441 atcctcgccc aggaagatgg accgggtgcg gtccaactgg ccgatcagtg ggtcaagtcc
    13501 caccctcagg acaccgtggc gcggcgggca ctggccgatg gtcacgcccg cgcgggcaac
    13561 ctgacggccg cccgcgcggc ctacgaggcg ctggtgaaga tggcgcccgg cgatgccgag
    13621 tcactgaaca acctcgccaa tgtgctgctg ctgaccaagg atccgggcgc actcaagatc
    13681 gccgagcagg ccttggcgct cgatcccaag gcgccccaca tcatcgggac ggtcggctgg
    13741 gcggccttca aggcggggca aacggaccgc gcgctccagt tcctgcgcga tgcacggctg
    13801 cgggaccccg acaaccgcga tacccgctat ttcctcggcg cggtactcgc cagttccggg
    13861 cgccccaccg aggcacgaga ggaactggaa gccgcactga agggcgacc
//
LOCUS       DAOORV010000097        13727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5480_length_13727_cov_6.212844, whole genome shotgun sequence.
ACCESSION   DAOORV010000097 DAOORV010000000
VERSION     DAOORV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_13727_cov_6.212844"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2160
                     /locus_tag="PKV56_12715"
     CDS             <1..2160
                     /locus_tag="PKV56_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOX68720.1"
                     /translation="RIGVSSLRLSGITCAACALPIEQALRGVDGVVEATVNAAAMRAS
                     VRWDPARTRPSALVDAVQRAGYDAVPDTAAAARESRRNESRQALWRLFVAAFCAMQVM
                     MLATPAYVAGPGGIEADLQHLLNWGSWVMALPVMTFSARPFFAGAWKALRLRHIGMDV
                     PVSLGILITFIASSGAAFDPQGPFGSTVYFDSLTMFVALLLASRWLEVRARHRAAEAF
                     ENALGALPETATRVLADGRTETIGIARLCIGDRVRVAVGEAFPADGNVLEGSTSADEA
                     LLSGESSPQGKTPGSDVVAGSVNLESPVIVQVLRTGADTRHEAIVALMREAQSQRPSS
                     ARWADRWAAPFLWAVLLLAGGAAAVWSVIDPSRALWVAVSVLIVTCPCALSLATPSAL
                     LAAANSLARRGVLLRRLEALESMAGCTRLFVDKTGTLTEDRPRYSGCDRLPAAALIDE
                     ATLLRMAASLAAWSRHPLAQALCESQPAPDADSTTWHALREQPGAGVEGQDAQGRRWR
                     LGSAHWVGHADGCAQENTGLETWFGPIGQPMARLRFDETVRSDVEAALSDLRAEGVRV
                     TLLSGDAPHRVDRMARLLGIESALGGSTPEAKLAAVRAAQGAGETVAMLGDGINDAPV
                     LAQADVSLAMGAGALLARGHADAVLLANRIGDVVELRRMSQRCVRVIRQNIAWAATYN
                     AVCVPLALVGMLPPWAAGIGMAASSLFVVCNALRLRR"
     gene            2170..2409
                     /gene="ccoS"
                     /locus_tag="PKV56_12720"
     CDS             2170..2409
                     /gene="ccoS"
                     /locus_tag="PKV56_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOX68721.1"
                     /translation="MDVLYLLVPMSLVAMLAILAVFAWALHGGQFEEIDAVGAQILEF
                     SDAPSGAVIDADQPASGRAREESGAVDVNRRRSSP"
     gene            2406..3854
                     /gene="ccoN"
                     /locus_tag="PKV56_12725"
     CDS             2406..3854
                     /gene="ccoN"
                     /locus_tag="PKV56_12725"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HOX68722.1"
                     /translation="MSLAQAPNAHAVAVYDDDPVRWFSLAAVLWGVVGMLVGVIIAAQ
                     LAWPELNFGLPWISFGRLRPLHTNAVIFAFGGCALMATSFHVVQRTCQTKLFAPALAR
                     FVFWGWQAVIVSAAVSLPLGLTQGKEYAELEWPIDILITLVWVAYAVVFFGTVGTRKV
                     RHIYVANWFFGAFILAVALLHLVNSAAIPAGAFKSYSAYAGVQDAMVQWWYGHNAVGF
                     FLTAGFLGMMYYYIPKQAERPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWAQS
                     IGMLFSLILLAPSWGGMINGVMTLSGAWHKLRDDPILKFLIVALSFYGMSTFEGPMMA
                     IKTVNGLSHYTDWTVGHVHSGALGWVGFVSMGSLYYLIPRMFGQTKMYSVRAIELHFW
                     IATIGIVLYIAAMWISGVMQGLMWRAVNPDGTLVYSFVESVKATFPFYVVRLLGGVLY
                     LTGMLIMAWNVVKTVSTGKAVPAPVPAVAVHA"
     gene            3868..4500
                     /gene="ccoO"
                     /locus_tag="PKV56_12730"
     CDS             3868..4500
                     /gene="ccoO"
                     /locus_tag="PKV56_12730"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HOX68723.1"
                     /translation="MNTKTNNHPPVSGHERIETNNLLMIVLILVVIAIGGIVEIVPLF
                     FQRSTTQPVEGLKPYTALQQAGRDIYTREGCYNCHSQMIRPLYAETLRYGHYSVAGEF
                     VWDHPFQWGSKRTGPDLHRVGGKYSDEWHKVHLNNPRDLVPESNMPAYPWLERTPVDA
                     ESMPQHLRALRTVGVPYTDQEIAQAGEAVKGKNELEALIAYLQVLGTSVK"
     gene            4508..4663
                     /locus_tag="PKV56_12735"
     CDS             4508..4663
                     /locus_tag="PKV56_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HOX68724.1"
                     /translation="MIDVNTLRIAVTMLSLGAFIGIVCWAWSRSNAASFAEAARLPLI
                     DDEGSTR"
     gene            4660..5574
                     /gene="ccoP"
                     /locus_tag="PKV56_12740"
     CDS             4660..5574
                     /gene="ccoP"
                     /locus_tag="PKV56_12740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HOX68725.1"
                     /translation="MSDFFSNGWSVFVAAASIVSMLLCLVLLATAARRRVMSNDNTTG
                     HVWDEDLQELNNPLPRWWMWLFIITVLFSFAYLALYPGAGSRAGTLGWSSAGQHQAEQ
                     DAARAAMQPVYARYASASAETLMQDPAAMGIGERLFVNNCAACHGSDAHGSKGFPNLT
                     DADWLHGGDHATIIKTITEGRVGMMPPLAAVVGDAKDLSNVAQYVLSLSGAKHDAAAA
                     TAGQAKFAVCAGCHGADGKGNQAVGAPNLTDKIWLHGFGEEAILAMINHGKTNVMPAQ
                     AQRLTTEQIHVLGAYVMSLSRNAAPATP"
     gene            5601..7049
                     /gene="ccoG"
                     /locus_tag="PKV56_12745"
     CDS             5601..7049
                     /gene="ccoG"
                     /locus_tag="PKV56_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOX68726.1"
                     /translation="MNRPVIPIVPAPAGGDGNGENVWLYEKRTQIYPRSVKGWFAGWR
                     WALVWLTQLVFYGTPWLTWNNRPAVLFNLEARRFYIFDLVLYPQDFIYLTALLIVCAY
                     SLFLFTAVAGRLWCGFACPQTVYTEMFLWVERHIEGDRMARMKRDAGPRSFDLIWRKS
                     AKHAAWLAIGLITGFTFVGYFTPIRTLGAEALSLGFGPWEWFWVLFYGFATYGNAGWL
                     REQVCLYMCPYARFQSAMFDHDTMIVSYDPQRGEPRGSRPRSADPRALGKGDCIDCSL
                     CVQVCPTGIDIRDGLQYACIGCAACIDVCDDVMDKMKYPRGLIRFATQNGLANRFDRK
                     QTLRRVFRPRVLIYSAVLLLICGAFVTSLALRSPFRVDVVRDRGALARMVGEGLIENV
                     YRLQVMNSTEAVQRFRVKVGGIDGATVTSRPDFEVGPAEARWLPVSVQISPETASKIG
                     PGVHQLHFEIETVDSDGDKAEVKEKSTFVVPR"
     gene            7075..7317
                     /locus_tag="PKV56_12750"
     CDS             7075..7317
                     /locus_tag="PKV56_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HOX68727.1"
                     /translation="MNTPAKTVNPPRSWWREPMMWIVIGGPAIVVVAAITTGFIAWNG
                     ADTVVLDPVAMSSSPVASNPGLEPALKARNHAATPR"
     gene            7401..8816
                     /gene="hemN"
                     /locus_tag="PKV56_12755"
     CDS             7401..8816
                     /gene="hemN"
                     /locus_tag="PKV56_12755"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOX68728.1"
                     /translation="MMFDPLTADRVGQLLSRLDVPGPRYTSYPTADRFTDTFGADQYR
                     QALQQRARDTQAGIVAPLSVYVHIPFCESICYYCACNKVITKHHESAGEYLTALRREV
                     ELHLDVLGRGAPLTQLHFGGGSPTFLSDDELAQLMTVLRDAFGIAPDAEVSIEVDPRT
                     VDEGRLRHLAHLGFNRLSFGVQDFDPDVQKAVHREQPYEAVATLIQQAREIGFKSINA
                     DLIYGLPRQTPATFARTVAQMGALRPDRIALYAYAHLPQRFKPQRRIHAHELPPAGER
                     VHMLRAAISGFQGLGYTHIGMDHFALPDDPLAVAKREGHLHRNFQGYSTQADCDLIGL
                     GVSAIGKIGRCYSQNDKTLPSYYEAIGRSQLAAVRGLELEPEDLLRRDVIMALMCQGR
                     VGFDEIERAHAVHMRKSFAGEIERLRPLEAQGLVAIEPAAIQVTPLGWYFVRAIAMVF
                     DRYLPVDIQREAHFQNYSRVV"
     gene            8916..9122
                     /locus_tag="PKV56_12760"
                     /pseudo
     CDS             8916..9122
                     /locus_tag="PKV56_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            9268..9546
                     /locus_tag="PKV56_12765"
     CDS             9268..9546
                     /locus_tag="PKV56_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOX68729.1"
                     /translation="MLLALPCGLLQSALLIAALANTPLQGALAMGSFAVASSLGLWLP
                     GTLAARAPGGASVNVLMLRLAGATLAAASAWALTHDLWLRWVAWCQTA"
     gene            9575..10303
                     /locus_tag="PKV56_12770"
     CDS             9575..10303
                     /locus_tag="PKV56_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX68730.1"
                     /translation="MPETTHIAIVDDEPDITQLLAGYLSRQGFRVSELSSGHALTALM
                     PVDPPDLVLLDLGLPGEDGFAIARTLREHWRCGLIILTGRGDAVDKVVGLEIGADDYV
                     TKPFDLRELLARIKAVLRRMTPAAPPPVEAAPVGRERLCFEHWELDVASRQLTGPEAR
                     PVALTAGEFDLLCVLARNAGRVLSRDFLLEQTRGREAAPFDRTIDVQIGRLRRKLEDD
                     PEEPRLIKAVRGAGYMLVPVVITR"
     gene            10320..12410
                     /locus_tag="PKV56_12775"
     CDS             10320..12410
                     /locus_tag="PKV56_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX68731.1"
                     /translation="MRRAATPPAATPAVASIYRSLFGAYPDGLLLVDSRGGIVMANPE
                     AERLLGYGSGELTGLTVDALVPDAIRPRHAAFREAYSREPKPRPMGTQMELVAKRRDG
                     REVMVEIALSPLEIEGQPFVVAAIRGIEGYPRVKQALQRARYSEFVAQMGRLAVDSRD
                     PQLLLQQVPAVAADALQVDAAVVFLLETSRLEFHVAGGVGLLSGEGLGSRQPNRPDTP
                     PGFILAHGRPVTVADYRAERRFTVPASYLSQGLVSALAVPLVDRGQIIGVLSVRSRQP
                     ERFGADELHFLESLSNLLAASLQRSQSEEALNHAQRMESVGQLTGGIAHDFNNLLTVI
                     QGNLQVLEEHPALEADAGAQQMVAAAARASRRGAELTSKLLAFSRRQQLSPSEVDVGE
                     LIESLADMLRRTLEQSIRIEVTADAAGLRCEVDAGQLESALLNIAINSRDAMPDGGTL
                     SFACAPCISFPPDVASDLGARARRGAFIEIVIRDTGEGMTESVRERVFEPFFTTKELG
                     RGTGLGMSTVYGFVKQSRGSIRLDTAPGAGTEVTIYLPRARGTAEPEGPGSDSAEGLP
                     DGLQVMLVEDDADVRCVVEQFLVSMGCVVSAFDHAEPALASLRAGERCQLLLTDIALG
                     AGMRGTELARQVRTRWPDLPVLLMSGYSSELLHAPGGREAEFELLRKPYEREDLARAI
                     ARVLKTGAPNSPPG"
     gene            complement(12436..13215)
                     /locus_tag="PKV56_12780"
     CDS             complement(12436..13215)
                     /locus_tag="PKV56_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX68732.1"
                     /translation="MQTLTHTSSVQSPAAKPTAAAGTVLVPAARRPTGERIAQLLALI
                     ETQLSVVRRVVRSGEPVYRAGDTFDSLYVVHAGMFKTVNVSADGREQVVGLHFKGDWL
                     GFDGIADGCFGCDAIAMDTGEVWMIRYDALLEGCSRTPTLMAAVHSAMSRELARDRDS
                     MMAMCTLPADARVADFLRNWADTLEERGLRTDCITLRMTRAEIGNFLGMKFETVSRAL
                     SRLARDKVIGFSEKGRRDVEIPDVAALSAFIDRSLAAATVH"
BASE COUNT         2210 a         4530 c         4584 g         2403 t
ORIGIN      
        1 cgcatcggtg tgtcttcact gcgcttgtcg ggcatcacct gcgctgcctg cgcgttgccg
       61 atcgagcagg cgttgcgcgg cgtcgacggc gtggtcgagg ccaccgtcaa tgcggcagcc
      121 atgcgtgcct cggtgcgctg ggacccggca cgcacccggc cgtcggccct cgtcgacgcg
      181 gtgcagcgcg ccggctacga cgccgtgccc gacacggccg ccgccgcccg cgaatcacgc
      241 cgcaatgaat cacgtcaggc gctgtggcga ctgttcgtcg cggccttctg cgcgatgcag
      301 gtgatgatgc tggcaacacc ggcttacgtg gccgggcctg gcggcatcga agcggatctg
      361 cagcacctgc tgaactgggg cagctgggtg atggcactgc cggtgatgac attctccgca
      421 cgccccttct ttgctggcgc ctggaaggcg ctgcgcctgc gtcacatcgg catggacgtt
      481 ccggtgtcgc tcggcatcct gatcacgttc attgccagca gcggtgcggc gttcgaccct
      541 caaggaccgt ttggcagcac cgtctatttc gattcgctga cgatgttcgt cgcgttgctg
      601 ctggccagcc gatggctcga agtgcgtgcc cgccaccgcg cagccgaagc cttcgagaac
      661 gcgctgggcg ccctgccgga aaccgccacc cgggtgcttg cagacggtcg caccgaaacc
      721 atcggcatcg cccggctgtg catcggtgac cgggttcgtg tggcggtcgg cgaggcgttt
      781 ccggcggatg gcaacgtgct ggagggcagc acttcggccg acgaggcgct gctgagtggc
      841 gagtcgtcgc cacagggcaa gacgccgggc tccgatgtgg tagccggcag cgtgaacctc
      901 gaatcgccag tgatcgtgca ggtgctgcga accggggccg acacccgcca tgaagccatc
      961 gttgcgctga tgcgtgaagc gcagtcgcaa cggccgtcga gcgcccgctg ggcggaccgc
     1021 tgggcagccc ccttcctgtg ggccgtgctg ctgctggccg gcggcgcagc agcggtgtgg
     1081 agcgtgatcg acccgagccg tgcgctgtgg gtcgctgtgt cggtactgat cgtgacctgt
     1141 ccctgcgcct tgtcgctggc cactccctcg gcattgctgg ccgcagcgaa ttcgctggcg
     1201 cgccgcggcg tgctgctgcg gcggctcgag gcactggaga gcatggccgg ctgcacgcgt
     1261 ctgttcgtgg acaagaccgg cacgctgacc gaggatcgcc cccgctacag cggctgtgac
     1321 cgcttgcctg ccgccgcact gatcgacgag gcgacgctgc tgcgcatggc tgcatcactg
     1381 gccgcgtggt cgcgtcaccc gctggcgcag gcactttgcg aatcgcagcc cgctcctgat
     1441 gcggacagca cgacctggca cgcgctgcgc gagcaacccg gcgcgggcgt ggaaggccag
     1501 gatgcccaag gccgtcgctg gcggctgggc agtgcgcact gggtcggcca tgccgatggc
     1561 tgcgcgcagg agaacaccgg gctcgaaacc tggttcggcc cgatcgggca gccgatggct
     1621 cgcctgcgct tcgatgaaac ggtgcgttcc gatgtcgagg ctgcgctttc agacttgcgc
     1681 gccgaaggcg tgcgcgtcac gctgctgtcg ggcgatgcac cccatcgggt cgatcggatg
     1741 gccaggttgc tgggcatcga atcggccctg ggcgggtcca cgccggaagc gaagctggcg
     1801 gcggttcgcg ccgcacaggg cgccggcgag acggttgcca tgctcggcga cggaatcaac
     1861 gatgcgcccg tgctggccca ggctgacgtg tcactggcca tgggcgcagg agccctgctc
     1921 gcacgcggcc atgccgacgc tgtgctgctg gcaaatcgca tcggcgacgt cgtcgaactg
     1981 cgccgcatgt cgcagcgctg cgtgcgcgtc atccgtcaga acatcgcctg ggcagccacc
     2041 tacaacgcgg tgtgcgttcc gctcgcgctg gtcggcatgc tgccgccctg ggcggccggc
     2101 atcggcatgg cggcgagctc gctttttgtg gtctgcaacg cgctgagact tcggcgctga
     2161 ggttctgaaa tggatgtgct ttatctgctg gttccgatgt ccctggtcgc gatgctcgcc
     2221 atcctggcgg tgttcgcatg ggcgctgcac ggcggacagt tcgaggagat cgacgccgtg
     2281 ggtgcgcaga ttcttgagtt cagcgatgcc ccgtcgggcg ccgtaattga tgccgatcaa
     2341 cccgcgtccg gcagggcgcg agaagaatcg ggcgctgtag atgtcaatcg aaggaggagt
     2401 tccccatgag tcttgctcaa gcgccaaatg cgcacgccgt agcggtctac gacgacgatc
     2461 ccgtccgctg gttctcgctc gcagccgtgc tgtggggggt ggtcggcatg ctggtgggcg
     2521 tcatcatcgc ggcccagctc gcctggcccg agctcaactt cggcctgccg tggatcagtt
     2581 tcggccgact gcggcccttg cacaccaacg cggtgatctt cgcgttcggc ggctgcgcgc
     2641 tgatggccac ctcgttccac gtcgtccagc gcacctgcca gacgaaactg ttcgcgcccg
     2701 cgctggcgcg attcgtgttc tggggctggc aggcggtgat cgtctccgcg gcggtctcgc
     2761 ttcccctggg tctcacgcag ggcaaggaat acgccgaact cgagtggccg atcgacatcc
     2821 tgatcacact ggtctgggtg gcttatgcag tcgtcttctt cggtaccgtc ggcacccgca
     2881 aggtgcgcca catctacgtg gccaactggt tcttcggcgc gttcatcctc gccgtggcgc
     2941 tgctgcacct ggtcaacagc gcagcgatac cggcgggcgc gttcaagagc tactcggcct
     3001 atgccggtgt gcaggacgcg atggttcagt ggtggtacgg ccacaacgcg gtcggcttct
     3061 tcctgacggc cggcttcctg ggaatgatgt actactacat ccccaagcag gctgagcgcc
     3121 ccgtctacag ctaccgcctg tcgatcgtgc acttctgggc gctgatcttc acctacatgt
     3181 gggcgggtcc ccaccacctg cactacaccg cgctgcccga ctgggcgcag tcgatcggca
     3241 tgctgttctc gctgatcctg ctggcgccaa gctggggcgg gatgatcaac ggcgtgatga
     3301 cgctgagcgg tgcctggcac aagctgcgcg acgacccgat cctcaagttc ctgatcgtgg
     3361 cactgtcgtt ctacggcatg agcaccttcg agggtccgat gatggcaatc aagacggtga
     3421 atggcctgag ccactacacc gactggaccg tcggccacgt gcacagcggt gcgctcggct
     3481 gggtcggctt cgtgtcgatg ggctcgctct actacctgat cccgcgcatg ttcggccaga
     3541 ccaagatgta cagcgtgcgg gcgatcgagc tgcacttctg gatcgcgacc atcggcatcg
     3601 tgctgtacat cgctgcgatg tggatttccg gcgtgatgca gggcctgatg tggcgtgcgg
     3661 tcaaccccga cggcacgctg gtctacagct tcgtcgaaag cgtcaaggca acgttcccct
     3721 tctatgtcgt gcgtctgctc ggtggagtgc tgtatctcac cggcatgctg atcatggcct
     3781 ggaacgtggt gaagacggtg tcaaccggca aggcggtccc cgccccggtg cccgctgtcg
     3841 cagtccacgc ttgaacggga gattcagatg aacaccaaga ccaataatca tcccccggta
     3901 tccgggcacg agcggatcga gaccaacaac ctgctgatga tcgtgctcat cctggtggtg
     3961 atcgcgattg gcggcatcgt cgagatcgtg cctctgttct tccagcgctc gaccactcag
     4021 ccggtcgagg gcctgaagcc ctacaccgca ctgcagcagg cgggtcgtga catctacacc
     4081 cgcgagggtt gctacaactg ccattcgcag atgatccggc ccctctatgc ggaaacgctg
     4141 cgctacggtc actattcggt ggcaggcgag ttcgtctggg accatccgtt ccagtggggc
     4201 agcaagcgca cagggcccga tctccatcgc gtcggcggca agtacagcga cgagtggcac
     4261 aaggtgcacc tgaacaaccc gcgcgatctg gtgcccgagt cgaacatgcc cgcctacccc
     4321 tggctcgagc gcacgccggt cgatgccgag tcgatgccgc agcacctgcg cgcgctgcgc
     4381 accgtcggcg tgccgtacac cgaccaggag attgcccagg ccggcgaggc cgtcaagggc
     4441 aagaacgaac tcgaagcttt gattgcctac ctgcaggtgc tcggcacctc ggtcaagtga
     4501 ggacaccatg attgatgtga acacactacg catcgccgtc accatgctgt cgctcggtgc
     4561 cttcatcggc atcgtgtgct gggcctggtc ccgcagcaac gccgcgagct tcgccgaagc
     4621 cgcccgactt cccctgatcg acgacgaagg gagcacgcga tgagcgactt cttctccaac
     4681 ggctggtcgg tgttcgtcgc cgcagcctcg atcgtgagca tgctgctgtg cctggtgctg
     4741 ctggcaacgg ccgcacggcg ccgcgtcatg tccaacgaca acacgacggg acacgtctgg
     4801 gacgaggatc tccaggaact caacaatccc ctgccgcgct ggtggatgtg gctgttcatc
     4861 atcacggtgc tgttcagctt cgcctatctg gcgctctacc cgggcgccgg cagtcgggcc
     4921 ggaacgctgg gctggtccag cgctggccag caccaggcgg aacaggacgc ggcgcgtgca
     4981 gccatgcagc ctgtgtatgc acgctatgcc agcgcatcgg ccgagacgct gatgcaggat
     5041 cctgccgcga tgggcattgg cgagcgcctg ttcgtcaaca actgcgcggc ttgccacggc
     5101 tcggacgcac acggcagcaa gggattcccg aacctgaccg acgccgactg gctgcacggc
     5161 ggtgatcacg caacgatcat caagaccatc accgaaggcc gggtcggcat gatgccgcca
     5221 ctggctgccg tggttggtga cgcgaaggac ctgagcaatg tcgcccagta cgtgttgagc
     5281 ctgtccggcg caaagcacga cgcagcagca gccacagcgg gtcaggccaa gttcgcggtc
     5341 tgcgccggct gtcacggtgc cgatggcaag ggcaatcagg ccgtcggcgc accgaacctg
     5401 acagacaaga tctggctcca cggcttcggc gaagaggcaa ttctcgcgat gatcaaccac
     5461 ggcaagacca acgtgatgcc agcgcaggcg caacgtctca cgaccgagca gatccatgtg
     5521 ctcggggctt acgtgatgag cctgtcccgc aacgccgccc cagcaacgcc ctgacggatc
     5581 agccaaatga agtaccccgc atgaaccgac cagtgattcc gatcgtcccg gcgccagccg
     5641 gcggcgacgg aaacggcgaa aacgtctggc tgtacgagaa acggactcag atctatccgc
     5701 gctcggtgaa gggctggttc gccggctggc gctgggcgct ggtgtggctc acgcaactgg
     5761 tgttctacgg cacgccgtgg ctcacctgga acaaccggcc ggcggtgctt ttcaacctgg
     5821 aggcccgccg gttctacatc ttcgatctgg tcctgtaccc acaggacttc atctatctga
     5881 cggcgctgct gatcgtctgc gcctactcgc tgttcctgtt caccgcggtg gccggccgcc
     5941 tgtggtgcgg cttcgcctgc ccgcagacgg tctacaccga gatgttcctg tgggtcgagc
     6001 ggcacattga aggcgaccgg atggcgcgca tgaagcgcga tgccggtccg cgatcctttg
     6061 acctgatctg gcgcaagagt gccaagcacg cagcctggct cgcgatcggg ctcatcaccg
     6121 gcttcacctt cgtcggctat ttcacgccga tccgcacgct cggggccgag gcgctcagcc
     6181 tgggattcgg tccgtgggag tggttctggg tgctcttcta cggctttgcc acctacggca
     6241 atgccggatg gctgcgcgag caggtctgcc tgtacatgtg cccctatgcg cgcttccaga
     6301 gcgcgatgtt cgaccacgac acgatgatcg tgagctacga cccccagcgt ggcgagcccc
     6361 ggggctcgcg cccgcgcagc gccgatccgc gcgcgctcgg caagggagac tgcatcgact
     6421 gctcgctgtg cgtgcaggtc tgcccgacgg gcatcgacat ccgggacggc ctgcagtacg
     6481 cctgcatcgg ctgcgccgct tgcatcgacg tctgtgacga cgtgatggac aagatgaagt
     6541 acccgcgggg actgatccgc ttcgcgaccc agaacggtct ggccaaccgc ttcgacagga
     6601 agcagaccct gcgacgcgtg ttccgacccc gggtgctgat ctacagcgcc gtgctgctgc
     6661 tgatttgcgg cgccttcgtc accagtctgg cgcttcgcag cccgttccgg gtcgatgtgg
     6721 tgcgtgatcg cggcgccctc gcacggatgg tcggagaggg gctgatcgag aacgtctacc
     6781 gtctgcaggt gatgaattcg accgaagccg tccagcgttt tcgcgtgaag gtcggtggca
     6841 tcgacggcgc gaccgtgacg agccggcccg acttcgaggt cggccccgcc gaggctcgct
     6901 ggctgccggt gagtgtgcag atcagccctg aaactgccag caagatcggc cccggcgtgc
     6961 accagctgca cttcgagatc gaaacggtgg attccgacgg ggacaaggcg gaagtgaaag
     7021 agaagtcgac gttcgtggtg ccgcgttgag cgccactttc caggagttgc cgagatgaac
     7081 acacctgcca agaccgtgaa cccgccccgt tcctggtggc gcgagccgat gatgtggatc
     7141 gtgatcggcg ggcccgcgat cgtggtggtc gcggccatca cgaccggatt catcgcctgg
     7201 aatggcgcag acacggtggt tcttgatccg gtggcaatga gttcttcgcc tgttgcaagc
     7261 aacccgggtc tcgagcccgc gctcaaggcg cgcaaccacg cggcgacgcc acgctgactc
     7321 ggcaggccgc gggctcgtcg acgagcccgc gaattgatcg acatcaaacc gtgcgttcgc
     7381 aggctaggca cagtgcgcgc atgatgttcg atcccctcac cgccgaccgg gtgggtcagt
     7441 tgctatcccg cctcgatgtg ccgggaccgc gctacacctc gtacccaacc gcagaccgct
     7501 tcaccgacac gttcggcgcg gatcaatacc gccaggcgct ccagcagcgc gcccgggaca
     7561 cgcaggccgg catcgtcgca ccgctgtcgg tctacgtgca catcccgttc tgcgagtcga
     7621 tctgctacta ctgcgcatgc aacaaggtga tcaccaagca ccacgagtct gccggcgagt
     7681 acctgaccgc gttgcgccgg gaagtcgagt tgcatctgga cgtgctcgga cgtggggcgc
     7741 cactgacgca gttgcatttc ggtggtggat cgccgacctt cctcagcgac gatgaactgg
     7801 cacagttgat gaccgtgctg cgcgatgcat tcgggatcgc gccggatgcc gaggtgtcga
     7861 tcgaggtcga cccgcgcacc gtcgatgaag gccggttgcg gcatctggcc catctgggtt
     7921 tcaaccggct cagtttcggc gtgcaggact tcgatccgga tgtgcagaag gccgtgcatc
     7981 gcgagcagcc ctacgaagcg gttgccacgc tgatccagca ggcacgcgag atcggcttca
     8041 agtcgatcaa tgccgacctc atttacgggc tgccgcgcca gacgccggcc acctttgctc
     8101 gcacggtggc acagatgggt gcgctgcggc ccgaccgcat cgcgctgtac gcctacgcac
     8161 acctgccaca gcgattcaag ccgcaacggc gcatccatgc ccacgagttg ccgccggccg
     8221 gtgagcgggt gcacatgctg cgtgctgcga tcagtggctt tcagggtctg ggctacacgc
     8281 acatcggaat ggaccatttc gcgctgcccg atgatccgct cgccgtggcc aagcgcgagg
     8341 ggcacctgca ccgcaatttc cagggctaca gcacgcaggc cgattgcgac ctgatcggtc
     8401 tcggggtctc ggcgatcggc aagatcggcc gctgctacag ccagaacgac aagaccctgc
     8461 cctcctatta cgaagccatc ggacgttcgc aactggcggc cgtgcgtggg ctcgaactcg
     8521 agcccgagga tctgctgcga cgcgatgtga tcatggcgct gatgtgccag ggtcgggtcg
     8581 ggttcgacga gatcgaacgc gcacacgcgg tccacatgcg caagagtttt gccggcgaga
     8641 tcgagcgcct gcggccgctc gaagcgcagg gtctggtcgc gatcgaacct gcggccatcc
     8701 aggtcacccc gctgggctgg tacttcgtgc gtgccattgc catggtcttc gatcgctacc
     8761 tgccggtcga catccagcgc gaggcgcatt tccagaacta ctcgcgcgtc gtttgacgcc
     8821 acccggcgta ccgcggtagc gttgcaccga tggataccgc cctgatactg ggagccggac
     8881 tgatgggtct ggctggcaca ccccattgcg cagcgatgtg cggagccccg tgtgccgcag
     8941 tcacccaacg atgcggcagc caggcgcgca acggcttgct ggccggcttc acgctgggcc
     9001 gcctgctcag ctacgccgcg ggcggagcag tcgtcgcggc cagcgtgggt gcggtgaacg
     9061 tgctgggttc gatggccccg gtgctgagac cgctgtggac attgcttcat ctcgcggtac
     9121 tcggcctcgg cctgtggctg ttcttcacag gccgccagcc tgcctggtgg gcccggatgg
     9181 gcgcggcgcc agtgatggca gccgcccccc ccccccgcgg gctggcagcg cgtggcagca
     9241 ccgctgcggg caggtgcagc cgggggaatg ttgctggcgc tgccctgcgg cttgctgcag
     9301 tcggcactgc tgatcgccgc cctggccaac acaccgctgc agggtgcgct cgcgatgggg
     9361 agctttgccg tggcttcgtc gctcggcctc tggctgccgg gcacgctggc tgcgcgagcc
     9421 ccgggcgggg cctccgtcaa cgtgctgatg ctgcgtctgg cgggagccac cctcgcggct
     9481 gcttcggcgt gggcattgac gcacgacctc tggctgcgct gggtggcctg gtgccagacg
     9541 gcctgacggc ctcggactgt cgcagaatca ccgggtgcct gaaaccaccc acatcgccat
     9601 cgtcgacgac gagcccgaca tcacccaact gctggcgggc tacctgtccc ggcagggatt
     9661 cagggtcagt gaactgtctt ccggccatgc gctgaccgcc ctgatgccgg tcgatccgcc
     9721 tgatctggtc ctgctcgatc tcggcctgcc cggcgaggac ggcttcgcga tcgcacgcac
     9781 gctgcgcgaa cactggcgct gcggactgat catcctcacc ggccggggcg atgcggtcga
     9841 caaggtggtg ggcctggaaa tcggcgctga cgactacgtg accaagccgt tcgaccttcg
     9901 cgaactgctg gcacgcatca aggccgtcct gcgccgcatg acaccggcgg caccgcctcc
     9961 ggtggaagcc gcacctgtcg gacgtgagcg gctgtgcttc gagcactggg agctcgatgt
    10021 ggcctcccgc cagttgaccg gccccgaggc acgccctgtc gcgctgacgg ccggcgagtt
    10081 cgatctgctg tgcgtgctgg cccgcaatgc gggtcgggtt ctttcgcgcg atttcctgct
    10141 cgagcagacg cgggggcgcg aggctgcgcc gtttgaccgg accatcgacg tgcagatcgg
    10201 gcgactgcgc cgcaagctgg aagacgaccc ggaggagcct cgcctcatca aggccgtgcg
    10261 gggcgccggg tacatgctgg tgcccgtggt gatcacccga tgagggcggg agcctgcgca
    10321 tgaggcgagc ggcgaccccg ccggcagcca cgcccgcagt ggcatcgatc taccgttcgc
    10381 tgttcggcgc ctatcccgac gggctgctgc tggtggacag ccgcggtggc atcgtgatgg
    10441 ccaacccgga ggcagaacgg ttgctcggct atggctcggg cgagctgacc ggcctcacgg
    10501 tggacgccct cgtgccggat gcgatccgcc cgcgccatgc ggccttccgc gaggcctaca
    10561 gccgggagcc gaagccgcgc ccgatgggca cccagatgga actggtcgcg aagcgtcgcg
    10621 atggtcgcga ggtgatggtg gagattgcgc tcagcccgct ggagatcgaa ggccagccgt
    10681 tcgtcgtcgc ggccatccgc ggaatcgagg gctacccccg ggtgaagcag gcgctgcagc
    10741 gggcccgtta cagtgaattc gtggcgcaga tgggacggct cgcggtggac tcgcgggacc
    10801 cgcaattgct gctgcagcag gtaccggcgg tcgctgcaga tgccctgcag gtcgacgcgg
    10861 ctgtcgtgtt cctgctcgaa accagccggc tcgaatttca tgtggcaggc ggtgtcggac
    10921 tgctgtcggg cgagggactg ggcagtcgcc agccgaaccg acccgatacg ccgccgggct
    10981 tcatcctggc tcatggccgt ccggtgacgg tggccgacta ccgcgccgag cgacgcttca
    11041 ccgtgccggc gtcctacctg tcacaggggt tggtgagtgc gctggccgtg ccgctggtcg
    11101 accggggcca gatcatcggc gtgctgtccg tgcgttctcg ccagcccgag cgcttcggcg
    11161 ccgatgaact ccacttcctg gagtcgctgt cgaatctgct ggcggccagc ctgcaacgct
    11221 cgcagagcga agaagcgttg aaccatgcgc agcgcatgga aagcgtcggg caactgaccg
    11281 ggggaatcgc tcacgacttc aacaacctgc tcactgtgat ccagggcaat ctgcaagtgc
    11341 tcgaggagca tcccgcactc gaggccgacg cgggtgcgca gcagatggtg gcagcggcgg
    11401 cccgcgccag ccggcgcggc gccgaactga cgagcaagct gctggccttt tcacgacgcc
    11461 agcaactcag cccctccgag gtcgacgtcg gcgagctgat cgaatcgctg gccgacatgc
    11521 tgcggcgcac gcttgaacaa tccatccgca tcgaggtgac ggcggacgcc gcgggtctgc
    11581 ggtgcgaagt cgatgccggg cagctggaat cggcgctgct caacatcgcg atcaactcgc
    11641 gcgacgcgat gcccgacggc ggtacgctgt cgttcgcctg tgctccctgc atctccttcc
    11701 caccggatgt ggcctcggac ctgggcgcac gcgcacgacg cggtgccttc atcgagatcg
    11761 tgatcagaga cacgggcgag ggaatgaccg aaagcgttcg agaacgggtc ttcgagccct
    11821 tcttcacgac caaggaactc gggcgaggaa cgggcctggg aatgagcacg gtctacggat
    11881 tcgtcaagca gtcgcgcgga tcgattcggc tcgacaccgc accgggtgcc ggcaccgaag
    11941 tcacgattta cctgccgcga gcacgcggca cggcagaacc cgagggcccg ggctcggaca
    12001 gcgccgaggg cctgcccgac ggactgcagg tgatgctggt cgaggacgac gccgacgtgc
    12061 gctgcgtcgt ggagcagttt ctggtgtcca tgggctgcgt cgtcagcgcc ttcgatcacg
    12121 ccgagccggc tctggccagc ctccgggccg gggagcgctg ccagttgctg ctgacagaca
    12181 tcgcgctcgg cgcaggcatg cgtggaactg aactggcgcg ccaggtccgg acacgctggc
    12241 cggacttgcc ggtgctgctg atgtcgggct actcgtccga actgctgcac gcccccggcg
    12301 gacgcgaggc cgaattcgaa ctgctgcgca agccttacga gcgcgaggat ctcgcccggg
    12361 ccatcgcacg cgtcctgaaa acgggcgctc cgaactcgcc gccgggctga tggcccgcca
    12421 ctgagcggaa gccgctcagt gcaccgtcgc cgcagccaga ctgcgatcga tgaaggcgct
    12481 gagggcggcg acgtccggaa tctcgacatc gcgccggccc ttttcggaga agccgatcac
    12541 cttgtcccgc gccagtctgg acagcgcccg gctgacggtc tcgaacttca tgccgaggaa
    12601 gttgccgatc tcggcacgcg tcatgcgcaa ggtgatgcag tcggtgcgaa gcccgcgctc
    12661 ctcgagcgtg tccgcccagt tgcgcaggaa gtcggccacg cgtgcgtctg ccggcaacgt
    12721 gcacatggcc atcatcgagt cgcggtcgcg cgccagttca cggctcatcg cggaatgcac
    12781 cgctgccatc agcgtcggcg tgcggctgca gccctcgagc agcgcgtcgt aacggatcat
    12841 ccagacttcg ccggtgtcca tggcgatggc atcgcagccg aagcaaccat cagcaatgcc
    12901 gtcgaagccg agccagtcac ccttgaaatg caggcccacc acctgctcac ggccgtccgc
    12961 cgagacgttc accgtcttga acatgccggc gtgaaccaca tacaggctgt cgaacgtgtc
    13021 tcctgcgcga tagaccggct cgcccgatcg caccacgcgc cgcaccacgc tcaactgcgt
    13081 ctcgatcagc gccagcagct gcgcgattcg ttcgccggtg gggcgccggg ccgccgggac
    13141 cagcaccgtg ccagcggcag cggtcggctt cgccgcagga ctttggacag acgatgtgtg
    13201 ggtgagcgtt tgcatggcgg cgtcctgatc aggttctggg aataggcgca ggttaggccg
    13261 cacccatcaa ccgccgaaga accttgcaaa tcgttcagta acgctgggtg aacgggaagt
    13321 aacagttttt cgcatcagca tgcacttcga ctgacatgga tcaagcacca agaccgcccc
    13381 agcacttatg ttgacgccgc gtcccacccg ggatttcggc catggagggg atcatggaag
    13441 tgctgccggt cgctgatgga agtcgtcgca gcgccacgct tcggcgggcc ggtggtggtt
    13501 tcgcgtgact gaacagcgac tgggcatgac gactccaccg tcatccgcac tccgcggcgc
    13561 agcacgtgca acagcgtttc cgggtgggga gcccgtcgga gcgcgcggca ccggaccccg
    13621 acacgccccc tgtgcagggc cgtgtcggct cgacgcaata atcggttcag gcacgcagtg
    13681 agcatctcgt tgaccgatcg cggcgaccga gcgcccgcct cccacgg
//
LOCUS       DAOORV010000098        13659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5520_length_13659_cov_10.585416, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000098 DAOORV010000000
VERSION     DAOORV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5520_length_13659_cov_10.585416"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1165)
                     /locus_tag="PKV56_12785"
     CDS             complement(275..1165)
                     /locus_tag="PKV56_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68733.1"
                     /translation="MTCAVSTFTHAQTAPTYADVAPLFAQRCVVCHSGEQAAAKLRLD
                     AHASVLAGGGRGPVVKPGNAAGSELIRRVKGQSQPRMPMTGPPWLSDAEVALLERWID
                     AGAQPAPSGGAAQASASPAAVLAPGPGEPVTWVHVAPVFAQRCAKCHTDGGQMGAPPE
                     GFRLTSYASALDAADRARIVPGQPEASELLRRIRGQALPRMPMDGPPYLSANEIGLVQ
                     RWIEQGARGADGKAAAVPVGANVRLHGTFRSGSRLDDLALIVGPGARLDKAPALGQRA
                     EVRGRLDAQGQVIVERIRAR"
     gene            1335..8615
                     /locus_tag="PKV56_12790"
     CDS             1335..8615
                     /locus_tag="PKV56_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOX68734.1"
                     /translation="MRKQLLQVFRVGSALCAVLVGGVASAQTCDDKNTVVADVVAIDM
                     PMVFNRLGAQNVNWQMYALRHDLVVRTPGQPPRPITDADFAEARKLLSSSQHVTLRPD
                     LRPRPLTLRVAAGECLRVNFTNLLTRISNPFEAHKQPLLPDGHPMDQRSDDPNMDIDD
                     QVASRTVGFHPNGLELVGSIASDSSHAGANATSLVPPGSSRTYTFFAPAEGSFLVSNP
                     GAAFGGEGTAGTSGTGLFGAVAVQPVGGRAYRGQVTEEELRLATRLLGDPALAGQQVQ
                     ACTHDAQRACTPSGQPIVHYDALYPAQSPWVDEGKAGRPILAMQQVQGGIRKLIHGDV
                     NAIIVGPGKDGSFPRSTYPLESTGVTNPSLPNRLEPFREFISVFHDENAAAQAFPGLF
                     EHPVLGHTLHGVRDAFMINYGSAGVGAEVVANRLKVGPMHDCVDCAFEEFFLSSFTVG
                     DPALLVDRPANLGLEPFTAGALAGPDLIGVSLDGQLAGGVAKQLGRLADELAKRMPGV
                     PLTGPKASKTYYPHDPANVHHSYQGDFVKFRNVHTGKEQHIFHLHNHQWLYNPNDDNS
                     NYIDAQAIGPGSGYTYEIAFGGSGNRNKSAGDAVFHCHYYPHFAQGMWYLWRIHDTFE
                     TGTALAVSGSEGRVPSFHKKPFDLADGTPAKGARALPDGEIAQGTPIPAVVPLPGKPL
                     ALMPGKVEVAANPRKLPDGRPVGSLAKVVDRERNPGFPFWIAGIEDTVGSRPPTPPLD
                     MLATVGGADGGVPRHTVEGYSAGSASKDTFTRLDASKKELLAKPKYFPETGTDIEQLA
                     MKFHAQVAHATSTVLPGGDVVAGTQFVTNGGKPEPGAPYFDPCVDDSRQRLSAGRLGE
                     FFGREKLSLSGASAFNADTPRIYKGANIQLDVTINKLGDHYPQQRILTLWEDVDATLA
                     TGFRTLPGAAEVKQPRPPEPLVLRMNTFDCARYLHTNLVPKEFEMDDYQVRTPTDIIG
                     QHIHLPKWDLPSADGSANGWNYEDGTFSPGMIRSRIHAINEWNAANPAARVPNPYAAG
                     QPAFDPTRPAVAVNDELHPRAHYYFGRGGPFQKDAKACEDAWRTLGYKAFHNESTEGM
                     ARPGMCDWLGARTTIQRWFSDPIINKEQQHRGLGITFTHDHLGPSTHQQVGLYATMLT
                     EPPASTWVHNETAELLYTRDQPAPMKCDDLDPATWRPDRRCDGGPTTWQAVIRSADEK
                     LHESHREFFLQFGDFQHAYRKDEYRGVDAQGVIVAKNAPGNEQAFRKAVTPSFRKPAS
                     PNFPDVIALPATCPGGKEELKLKRRGANGQIEENVAATVPHQIPRPCPEIISADDVGT
                     MVVNYRQEPVAARVMNLGGNAVHNPGAAQASQTSGRAGDLAFAYQTRTDRALAALNTA
                     NGESTYAPLTHDVRPGDPYTPLLRAYSGDLVRIKIQAGSHEHEHNTAINGLKWLQAGS
                     GFGQAPHSGWRGGQNIGLSEQFTLSTRITDYFNSDRINSTDRLYATDTSQDGLWNGVW
                     GFVRTLERRNPAEDKRPAERDDQDRLAILPANPLPTLFAPQSKDMRRNDCPAEAPVRK
                     YHLVAGLANRLLGPAANVALRAVGGVDAAGGTLVYNPRPNEITLRVFGEADDPATPVD
                     ERTVVQRTERFGVGPLHDPTAILIARLQDVDANTRKLKAGVPVEPIVLRAAAGECVEV
                     TLENWLPMRRSAMPDLAGYTTLSMLMPKKMGAGSDEHESFNNNHVRASNHIGLHPQMV
                     QYDPQLHDGTVVGGNRTSLVSPGHSIKYKWYAGELSFTPGAGGAPQCTVTAGTEGGYE
                     LVNVLRDQERQTLDGVRMIRPPNQLFTREALLARLEALLSPVGEDGTEPAADDTEALT
                     VTTASGRTAVAAAALRPRVLALDNATLATLRRVQPSAARLAVTAAPPQGMTAAVFDSL
                     LASGWKSLSAAVEAADCFEQAIDGERLAENVPDEVTANLPALLGTPAGIAAPALQSAP
                     LFTAASAFSSVEALQRREACAQRLRQPMIDALAPVLRGTQFSERSDRVAAQLASSIAY
                     LVPDLDADALGSPEYAKAFQWLEGRSRAPLDPKTTRLVGPAGAGGQCQFAAIEFGGTN
                     LTPPDRIKQGQKAAVGAFVIEPERSLWWETDTALDRQNPGPAPGAKRATRTSAHVFYP
                     ASVQGTRTGDWRYFRDLVAVHQKGLNMRYGKRATGKSSAVGSIAAEREDTWQAPDRIA
                     PEDAHDSGQMAINYGSEPMWFRFGMDPSAPFGHGPSMSNPSVPGEGFGALRMAFMGFA
                     DSCCLGEAGQSLPASPGPYTPIFKVPPLAETRLRVMMPTGVGRASVMTLHGHGWQRDP
                     HLAERTVAAPLPMGQFGQPGARLAGGLHMAATTPVQYGVPSQCQGRNALGMWLGAQDS
                     VSPMAHHDWVLEHAGGFMGVAGDYLMRDIGGFGVTSGLWGLMRVQGNPVPMALRKGLR
                     TACH"
     gene            8606..10516
                     /locus_tag="PKV56_12795"
     CDS             8606..10516
                     /locus_tag="PKV56_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68735.1"
                     /translation="MPLSHAVVAAAGTWRRAVAGAVVALVPLAHAAADVSGARWERQL
                     EQDGVTATVSIEPLTADGLQGGAPARVRVTLSSTADGSALPRLLPGVWFDAQERAGAE
                     GGDAAACQRRVARYARGSGINPQALVDLNGYDVLALNADASISVLDPRTQFAGKTSLR
                     ATLPLPGPGFDWAATADDRLLWVTVPGSRAVVLADLAALRSLQTITLPGRPGRVRVEP
                     GGARAWVGVLAAEGEAQRGGVAVVEASAPYRQQWLPLSGRGHVEMAFDPAGWVALTQR
                     DAGEVVFVDPTTLQVAHRERLDAAGAMPLSVVFDAAARRFVVAEARGGALWVFDHRGR
                     LQDRLNLAPGIGPMAVSPDGRWLLVANPAAHQVDVIDLPAWRHAHRVPVSGRPFDIGM
                     TGAYAYVRALDSEAVSLIALASLAAAPRVQPIAMGERAPGLTPQLPLASQLVPMPDGT
                     GSFVASPGDNAVYFYMEGMNAAAGSVSARGHEVRAVRVARRGLREVAPGVHEMQVELP
                     DTPHMVVALATESPRTRQCTALALKPAPTAAGEGWQLAWEQFPVQHGRPLSVRVHGVA
                     VESLPAALHLRLFQPGAAALQLVAHADGGGRYSAEVPELADGLWYVHPQTPAGARTPW
                     GYVSFMRGAATR"
     gene            10527..11066
                     /locus_tag="PKV56_12800"
     CDS             10527..11066
                     /locus_tag="PKV56_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOX68736.1"
                     /translation="MLLARRSVLALGLGLAAGARAAAPSVPDAPLWHRDGRQVRLRSD
                     VWRDRIALVNFVYTSCSSFCGVQSAMLAELQSRLGERMGREVVLISLSIDPLTDDPPR
                     MQSFARPFEPGPHWWWLTGEPRTMFKTLEALGADRGDPRDHAPLWLAGTSQSPRRLVG
                     LPSLKQLETALGTPPSSPR"
     gene            11063..11665
                     /locus_tag="PKV56_12805"
     CDS             11063..11665
                     /locus_tag="PKV56_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOX68737.1"
                     /translation="MKTPDGPLALMSRRASLRCLAGCTLGLTVGAAGAQARSDKARAW
                     FTDTTLIDQDHRPHAFYSDLLAPRAVLINAAFVGCSSACPLLTQQLVKVREGLGDRFG
                     RDIWFLSITVDPLSDGPDELKRFAQRQGADAPGWRFLTGAPQDVKLVLGRLGLWVESP
                     DAHQTGLIAGRAAGGHWGKLRPDGSSQALVQQLARFLAPA"
     gene            11685..13067
                     /locus_tag="PKV56_12810"
     CDS             11685..13067
                     /locus_tag="PKV56_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOX68738.1"
                     /translation="MLAIALVFGALAVLAPVRAASTDGGRELFERGTTGGQSLSARTG
                     ADRSWVLRGATVACANCHGLAGTGGGEGYLRAPDLRWPQWSSPDPRQHAAARERLRRA
                     LRQGIGGDGRTLGAAMPRFDLDDASLDALAGHLQRLATERTPGSRPRLAVMQLDDGQQ
                     GPTESELGPALRACLRQRLGDRAEVEFVMVASPAEAQAQWRQWQQRSEVMAVLAPPWR
                     GWRPPAPAEGTVALAALFPLVADPETGRVQSVQWLFGGVEARAVALVQAWLQQPQAQS
                     ATLPVWVGEGALAPAAPEMLERMAQGVRRDTGRTLAWQVLDSPRLRTGEVGLWLDAQR
                     PPGDGWWLLPHGATASPGPAARWWVAAPFAGHPPRSLAQRWADATCRTAQAAIADQPV
                     LSRERWLPAVANTARLRDGAGWEWHVPAHDEYGYGASTAWTIVEIAGGTEPTPVAPQV
                     NIGRPIEAAR"
     gene            complement(13088..13459)
                     /locus_tag="PKV56_12815"
     CDS             complement(13088..13459)
                     /locus_tag="PKV56_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68739.1"
                     /translation="MSNITLTRRQNALALFQDYAEKALASGGSPKGLEQSFASTLQIS
                     PSMWSQIKSSRPIGDKLARQIESASGKPSGWLDELRKDAAPTAAEAAFLELALAAWRA
                     SNGAGRRAMRDQLKGFLSASG"
BASE COUNT         2153 a         4763 c         4647 g         2096 t
ORIGIN      
        1 actcgccgcg aaccttcatg aaggtcaccc agtatttcat cctcgactac ctcctccgtc
       61 gattgaaagc tcaccccgca gcgacagctt acggcgctgg atgctcggtg agaaagaacg
      121 ccgagatgca cgttgggaac cttccgcgta ggcaaggggg acggcacatc cgcgggcatc
      181 gaagttcgct aggcttgccg ccagcatttg gctgccaggc tgcgctgacg cacaccgccg
      241 agcgatagag gcgtcggccg cgttcacgct cacgctatcg agcccggatc cgctcgacga
      301 tcacttggcc ttgcgcatcg aggcgaccgc gaacttccgc cctttggcca agggccggtg
      361 ctttgtcgag tcgcgcaccc ggaccgacga tcagtgccaa gtcgtcgagt cgcgacccgg
      421 accgaaaggt gccgtgcagg cgaacattgg cacccacggg aacggcggcc gcctttccat
      481 cggcaccgcg cgcaccctgc tcgatccagc gttgtacgag tccgatctcg ttcgcgctga
      541 ggtaaggagg gccgtccatc ggcatgcgtg gcagcgcctg accgcgaatg cgtcgcagca
      601 gttcgctggc ctccggctgg cccggcacga tgcgtgcgcg atctgctgcg tcgagtgctg
      661 aagcgtatga agtcagtcga aatccctcag gcggtgcgcc catctggccg ccgtcggtgt
      721 ggcacttcgc gcaacgctga gcgaagacgg gagccacgtg tacccaggtg acaggctcgc
      781 ccggcccggg tgccaatacc gcagcgggcg aggccgaggc ctgggctgca ccgcccgacg
      841 gtgctggctg cgcgccggcg tcgatccatc gctccagcaa ggcaacctcg gcatcgctca
      901 gccagggagg gccggtcatc ggcatgcgtg gctggctctg ccccttcacc cgacggatca
      961 actcgctgcc cgccgcgttg cccggcttca ccacaggccc gcggccgccg ccggcgagga
     1021 ctgaggcatg cgcgtcaaga cgcagcttgg ccgcggcttg ttcgcccgag tggcagacca
     1081 cgcagcgctg tgcaaacagc ggtgcgacgt cggcatacgt cggtgctgtc tgcgcatgcg
     1141 tgaatgtgct gacagcacag gtcatcgcca atccgacccg gcgtatcgct ccacagaagt
     1201 aatgaagaca tttgctcata ggggggtgcc tccctaacgg tagtggcatc gcagatcacc
     1261 tcgatctgtt cgaatggtat ggaacccgtc atggcgtgac catgcgtgca ccgtctgttc
     1321 accggagacc cgcgatgagg aagcagttac tccaagtctt cagggttggt tccgcactct
     1381 gcgccgtgct ggtggggggg gtggccagtg cccagacctg cgacgacaag aacacggtgg
     1441 tcgccgacgt cgtcgccatt gacatgccga tggtgttcaa ccgcctgggc gcgcaaaacg
     1501 tgaactggca gatgtacgcg ctgcggcatg acctcgtggt acgcacgccc ggtcagccgc
     1561 cgcggcccat cacggacgcc gacttcgccg aggcgcgcaa gctgctcagc agcagccagc
     1621 acgtcacgct gcggcctgac ctgcgcccgc ggccgctcac gctgcgcgtg gcggcaggcg
     1681 aatgcctgcg ggtgaacttc accaacctgc tcacacgcat ctccaatccg ttcgaggcgc
     1741 acaagcagcc gctgctgccc gacggccacc cgatggatca gcgttcggac gacccgaaca
     1801 tggacatcga cgaccaagtg gcaagccgca ccgtcggctt ccatcccaac ggcctcgagc
     1861 tggtcggcag catcgcctcg gacagctctc acgccggcgc caacgccacc agcctggtgc
     1921 cgcccggcag ctcgcgcacc tacaccttct tcgcaccggc cgagggctcc tttctggtca
     1981 gcaatcccgg cgcagcattc ggcggcgagg gcacggcagg caccagtggc acagggctgt
     2041 tcggggcggt tgccgtgcag ccggttgggg gccgtgccta ccgcggacag gtcaccgaag
     2101 aggaactgcg tctggccaca cgattgctcg gcgatccggc actcgccgga caacaggtac
     2161 aggcgtgcac ccacgatgcg cagcgcgcgt gcacgccaag cggccagccg atcgttcact
     2221 acgacgcgct gtatcccgcg cagtcgccct gggtcgatga aggcaaggcc ggccgcccga
     2281 ttctcgcgat gcagcaggtg caaggcggca tccgcaagct gatccacggc gacgtcaacg
     2341 ccatcatcgt tggtcctggc aaggacggga gcttcccgcg cagcacctac ccgctggaaa
     2401 gcactggcgt caccaatccc agcttgccca accggctgga gccgttccgc gagttcatca
     2461 gcgtctttca cgacgagaac gccgcggcgc aggccttccc cggactgttc gagcacccgg
     2521 tgcttggtca cactcttcac ggtgtgcgcg atgccttcat gatcaactac ggcagcgcgg
     2581 gtgtaggtgc cgaagtggtg gcgaaccggc tcaaggttgg cccgatgcac gactgcgtcg
     2641 actgcgcttt cgaggagttc ttcctctcgt cattcaccgt cggcgacccc gcactcctcg
     2701 tcgatcgccc agccaatctt ggcctggagc ccttcacggc aggagcactc gccgggccgg
     2761 acctgatcgg cgtgagcctc gatggtcagc tggccggcgg cgtcgccaag cagcttggtc
     2821 gcctggccga cgaacttgcc aaacgcatgc ccggcgtgcc tctcactggc ccgaaggcga
     2881 gcaagaccta ctacccgcac gacccggcca acgtgcacca cagctaccag ggggacttcg
     2941 tcaagttccg caatgtgcac acgggcaagg aacagcacat cttccacttg cataaccacc
     3001 agtggctcta caacccgaac gacgacaact cgaactacat cgacgcgcag gcgatcgggc
     3061 cgggctcggg ctacacctac gaaattgcct tcggcggctc gggcaaccgc aacaagagtg
     3121 caggtgatgc cgtcttccat tgccactact acccgcactt cgcgcaaggc atgtggtacc
     3181 tctggcgcat ccacgacacc ttcgagactg gcacggcgct ggccgtcagc ggcagcgaag
     3241 ggcgcgttcc gtctttccac aagaagccct tcgacctggc tgacggcacg ccggccaaag
     3301 gcgcacgtgc actgcccgat ggcgagatcg cgcagggcac gccaatcccc gccgtggtgc
     3361 cgttgccggg caaaccgctg gcgctgatgc ccggcaaggt cgaggtggca gccaacccgc
     3421 gcaagctgcc cgacgggcgg ccggtcggtt cgctggccaa ggtggtcgat cgtgagcgca
     3481 atccgggctt cccgttctgg atcgccggga tcgaggacac ggtgggctcg cgcccgccga
     3541 cgccgccgct ggacatgttg gccacggtgg gcggcgccga tggtggtgtg ccgcggcata
     3601 ccgtcgaggg ctactccgct ggcagcgcgt cgaaagacac attcactcgg ctggacgcct
     3661 cgaagaagga attgctcgcc aagccgaagt acttccctga gaccggcacc gacatcgagc
     3721 agctggcgat gaagttccac gcccaagtgg ctcacgccac ctcgacggtg ctacccggcg
     3781 gcgatgtcgt ggccggtacc cagttcgtca ccaacggcgg caagcccgag ccgggcgcgc
     3841 cgtacttcga cccctgcgtc gatgacagcc gccagcgcct gtcggcgggt cgtttggggg
     3901 agttcttcgg ccgcgagaag ctcagcctgt cgggcgccag tgctttcaat gccgacacgc
     3961 cgcgcatcta caagggcgcc aacatccaac tcgacgtcac catcaacaag ctcggcgacc
     4021 actatccgca gcagcgcatc ctcacgctgt gggaagacgt ggatgccacg ctggccacgg
     4081 gcttccgcac gctgcccggc gcagccgagg tgaagcagcc gcgcccgccc gagccgctgg
     4141 tgctgcgcat gaacaccttc gactgcgccc gctacctgca cacgaacctg gtgcccaaag
     4201 agttcgagat ggacgactac caggtgcgca cgccgaccga catcatcggc cagcacatcc
     4261 atctgccgaa gtgggacctc ccgtccgcag acggttcggc caacggctgg aactacgagg
     4321 acgggacctt ctcgcccggc atgatccgca gccgcatcca cgccatcaac gagtggaatg
     4381 ccgccaaccc cgcagcgcgc gtgcccaatc cctatgcggc cggccagccg gccttcgatc
     4441 cgaccaggcc ggccgtggcg gtgaacgacg agcttcatcc gcgtgcgcac tattacttcg
     4501 gccgcggcgg tccgttccag aaggacgcca aggcgtgcga ggacgcctgg cggacgctgg
     4561 gctacaaggc cttccacaac gaatcgaccg agggcatggc gcgccccggc atgtgcgact
     4621 ggctcggtgc gcgcaccacc atccagcgct ggttctccga ccccatcatc aacaaggagc
     4681 agcagcaccg cgggctgggc atcaccttca cgcacgatca cctggggcct tccacgcacc
     4741 agcaggtggg cctgtacgcc accatgctca cggagccacc ggcgtccact tgggtacaca
     4801 acgagacggc cgaactgctg tacacgcgcg accagccggc acccatgaag tgtgacgatc
     4861 tcgaccctgc gacgtggcgg ccagaccgcc gctgcgatgg cggcccgacc acctggcagg
     4921 ccgtgatccg atcggccgat gaaaagctgc acgaatcgca ccgcgagttc ttcctgcagt
     4981 tcggcgactt ccagcatgcc taccgcaagg atgagtaccg cggtgtcgat gcgcagggcg
     5041 tgatcgtggc caagaatgcg ccgggcaacg agcaggcctt ccgcaaggcc gtgacgccgt
     5101 cgttccgcaa gccggcctcg cccaacttcc ccgatgtgat cgcgctgccg gccacctgcc
     5161 ccggcggcaa ggaggagctc aagctcaagc ggcgtggcgc gaacggccag atcgaggaaa
     5221 acgtggcggc cacggtgcct catcagattc ccaggccctg tccggagatc atctcggccg
     5281 acgacgtcgg caccatggtc gtcaactacc gccaggagcc ggtggcggcg cgagtgatga
     5341 acctcggcgg caatgccgtg cacaaccccg gcgcggcgca ggcttcgcag acctcgggcc
     5401 gcgccggcga cctggccttc gcctaccaga cgcgaaccga ccgcgcgctg gccgcactga
     5461 acaccgccaa tggtgagagc acctacgcgc cgctgacgca cgacgtgcgc cccggcgacc
     5521 cctacacgcc gctgctgcgc gcctattcgg gtgaccttgt acgcatcaag atccaggccg
     5581 gctcgcacga gcacgagcac aacacggcca tcaacggctt gaagtggctg caggccggca
     5641 gcggcttcgg gcaggcacca cactccggtt ggcgtggcgg ccagaacatc ggcctgtcgg
     5701 agcagttcac gctgagcacg cgcatcaccg actacttcaa ctcggatcgc atcaacagca
     5761 ccgatcgcct gtatgccacc gacaccagcc aggacggcct gtggaatggt gtctggggct
     5821 tcgtgcgcac gctggagcgg cgcaacccgg ccgaagacaa gcggcctgcc gaacgcgacg
     5881 atcaggaccg actcgccatc ctgccggcca acccgttgcc gacgctgttc gcgccgcagt
     5941 cgaaggacat gcgccgcaac gattgcccgg ccgaggcgcc ggtgcgcaag taccacctgg
     6001 tggcgggcct ggctaatcgc ctgcttggcc cagccgccaa cgtggcgctg cgcgccgtcg
     6061 gaggcgtcga tgccgccggt ggcacgctgg tctacaaccc gcggcccaac gagatcacgc
     6121 tgcgcgtgtt cggcgaggcg gatgaccccg ccacaccggt ggacgagcgc accgtcgtgc
     6181 agcgcaccga gcgcttcggc gtcgggccgt tgcacgaccc gacggcgatc ctgatcgcgc
     6241 gcctgcagga cgtcgacgcg aacacccgca agctcaaggc cggcgtgccc gtcgagccca
     6301 tcgtgctgcg cgccgcagcc ggcgaatgcg tcgaagtcac gctcgagaac tggctgccga
     6361 tgcgccgcag cgcgatgccc gacctggccg gctacaccac gctgagcatg ctgatgccga
     6421 agaagatggg tgccggcagc gacgagcacg agtccttcaa caacaaccac gtgcgcgcgt
     6481 ccaaccacat cggcctgcat ccgcagatgg tccagtacga cccgcagctg cacgacggca
     6541 cggtggtcgg cggcaaccga acctcgctgg tgtctccggg ccactccatc aagtacaagt
     6601 ggtacgcggg cgaactgagc ttcacgccgg gggcgggcgg tgccccgcaa tgcaccgtga
     6661 cggcaggcac cgagggtggc tacgaactcg tgaacgtgct gcgcgaccag gagcgccaga
     6721 ccctggacgg cgttcggatg atccggccgc cgaaccagct gttcacgcgc gaggcgctgc
     6781 tggcccgact cgaggcgctg ctgtctccgg tgggcgagga cggcacagag ccggcggccg
     6841 acgacaccga ggcgctgacc gtcaccacgg cgtcggggcg gacagcggtc gcagcggcgg
     6901 cactgcggcc aagagtgctc gcgctggaca acgccacgct ggccaccctg cgccgcgtgc
     6961 agccgagtgc cgcgcggctg gccgtgacgg cagcgccgcc gcaaggcatg actgcggctg
     7021 tgttcgacag tctgctcgca tcgggctgga agtcgctgtc cgccgcggtg gaagcggcgg
     7081 attgcttcga gcaggcgatc gacggtgaac ggctggccga gaacgtgccc gacgaggtga
     7141 ctgccaacct gcccgcgctg cttggcactc ctgctggcat cgccgcgccg gcgctgcagt
     7201 cggcgccgct gttcacagcc gcatcggcgt tcagcagcgt cgaggcgctg cagcggcgcg
     7261 aggcctgtgc gcagcggctg cgccagccga tgatcgatgc tctggcgccg gtgctgcgcg
     7321 gcacgcagtt ctccgagcgc agtgacaggg tcgcggcgca gctggcgtcg agcatcgcct
     7381 atctggtgcc ggatctcgac gccgatgcgc tggggagccc cgagtacgcc aaggcgttcc
     7441 agtggctcga aggccgctcg cgcgcgccgc tggatccgaa aaccacgcgc ctggtcgggc
     7501 ccgcaggggc ggggggacag tgccagttcg ccgccatcga gttcggtggc accaacctca
     7561 cgccgcccga ccgcatcaag cagggccaga aggcggcggt gggggccttc gtcatcgagc
     7621 cggagcgcag cctctggtgg gaaaccgaca ccgcgctgga ccgtcagaac cccgggccgg
     7681 caccaggagc caaacgggct acgcgcacca gtgcccacgt gttctacccg gccagcgtgc
     7741 aagggacgcg caccggcgac tggcgctact tccgtgatct cgttgccgtg catcagaaag
     7801 gtctgaacat gcgctatggc aagcgcgcga cgggcaagtc gtcggcggtg ggctccatcg
     7861 cggcagagcg cgaggacacc tggcaggccc ccgatcgcat cgcgccggaa gacgcccacg
     7921 attccggcca gatggcgatc aactacggca gcgagccgat gtggttccgc ttcggcatgg
     7981 atccgtcggc gcctttcggc cacggtccga gcatgagcaa ccccagcgtg ccaggcgaag
     8041 gcttcggtgc cttgcgcatg gccttcatgg gttttgccga cagctgctgc ctcggcgagg
     8101 ccggtcagtc attgcctgct tcccccggcc cgtacacgcc gatcttcaag gtgccgccgc
     8161 tggccgagac gcgcctacgg gtgatgatgc ccaccggcgt cggccgtgcc agcgtgatga
     8221 cgctgcacgg ccacggctgg cagcgcgatc cgcacctggc cgagcgcacg gtcgccgcac
     8281 cgctgccgat ggggcagttc ggccagccgg gcgcgcgcct ggccggcggg ctgcacatgg
     8341 cggccacgac accggtgcag tacggtgtgc ccagccagtg ccagggccgc aacgcgctgg
     8401 gtatgtggct gggcgcgcag gacagcgtgt cgccgatggc gcaccacgac tgggtgctcg
     8461 agcatgcggg tggcttcatg ggtgtggcgg gtgactatct gatgcgcgac atcggaggct
     8521 tcggcgtgac cagcggcttg tgggggctga tgcgcgtaca gggcaatccg gtgccgatgg
     8581 cgcttcgcaa gggcctgcgg acggcatgcc actgagtcac gctgtcgtcg cagcagccgg
     8641 cacatggcgc cgggcggttg ctggcgccgt cgttgcgctg gtgccgctcg cgcacgcggc
     8701 ggccgatgtg tcgggcgcgc gctgggagcg gcagctcgag caggacggcg tgaccgccac
     8761 ggtgtcgatc gagccgttga cggcggatgg gctgcagggc ggcgcgccgg cacgcgtgcg
     8821 cgtgacactg agcagcacgg ccgacggcag cgcattgccg cggctgttgc ctggggtgtg
     8881 gttcgatgcg caggagcgcg cgggcgccga aggcggcgat gcggcggcgt gccagcgccg
     8941 cgtggcacgc tacgcgcgag gcagcggcat caatccgcag gcactggtcg acctcaacgg
     9001 ctacgacgtg ctggcgctca acgccgacgc cagcatctca gtgctcgatc cgcgcacgca
     9061 gttcgccggc aagaccagct tgcgcgccac gctgccgctg cctggtcccg gcttcgattg
     9121 ggccgcgacc gccgacgatc ggctgctgtg ggtgacagtg ccaggctcgc gtgcggtcgt
     9181 gctcgccgac ttggcggcct tgcgcagcct gcagaccatc acgctgcccg gtcgccccgg
     9241 ccgcgtgcgc gtcgagccag gtggggcgag ggcctgggta ggtgtcctcg ctgccgaggg
     9301 cgaggcccag cgcggcggcg tggcggttgt ggaggcatcg gcgccgtacc gccaacagtg
     9361 gttgcccctg tcggggcgag gccatgtcga gatggccttc gatccggccg gatgggtggc
     9421 gctgacccag cgcgatgccg gcgaggtggt gttcgtcgat ccgaccaccc tgcaggtggc
     9481 gcaccgcgag cgcctggacg ctgccggtgc catgccgctg agcgtcgtgt tcgatgccgc
     9541 agcacggcgg ttcgtggtgg cggaggcgcg cggtggcgcc ctatgggtct tcgatcatcg
     9601 cggtcggctg caggaccggc tgaacctggc tcccggcatc gggccgatgg ccgtctctcc
     9661 cgatggccgc tggttgctcg tggccaaccc ggccgcgcac caagtcgacg tgatcgacct
     9721 gccggcctgg cgccatgctc atcgcgtgcc ggtcagcggc cggcccttcg acatcggcat
     9781 gacaggcgcc tatgcctatg tgcgcgcgct cgacagcgag gccgtctcgc tgatcgcgct
     9841 ggcgtcgctg gccgctgcgc cgcgcgtgca gcccatcgcg atgggcgagc gtgcgcccgg
     9901 gctcacgccg caactgccgc tggccagcca actggtgccc atgcccgacg gcaccggtag
     9961 ctttgtcgcc agcccgggcg acaacgcggt ctacttctac atggagggca tgaacgccgc
    10021 tgccggcagc gtgtcggcgc ggggccacga ggtgcgtgcg gtgcgggtgg cacggcgcgg
    10081 gctgcgcgaa gtggcgcccg gcgtccacga aatgcaggtc gagctgccgg atacgccgca
    10141 catggtggtc gcgctggcca ccgagtcgcc gcgtacgcga cagtgcacgg ccctcgccct
    10201 caagcccgcg cccacggcgg cgggcgaagg ttggcaactg gcctgggagc agtttcccgt
    10261 gcagcacggg cggccgctca gcgtgcgcgt gcatggcgtc gccgtcgaat ccttgccggc
    10321 ggctctgcat ctgcgcctct ttcagcccgg cgccgccgcg ttgcagctcg tcgcacatgc
    10381 cgacggcgga ggccgataca gcgccgaggt gcccgaactg gccgatgggc tctggtacgt
    10441 tcatcctcag acccctgccg gcgcccgcac gccttggggc tacgtcagct tcatgcgcgg
    10501 cgctgccacg cgctgaagga tcatcgatgc tgcttgcccg ccgttccgtc cttgcccttg
    10561 ggctcggcct cgccgcaggc gcgcgcgccg ctgcgccctc agtacccgac gcgccgttgt
    10621 ggcatcgcga tggccgccag gtccggctgc gcagcgatgt ctggcgcgat cgcatcgcgc
    10681 tggtcaactt cgtctacacg agctgctcga gcttttgcgg cgtgcagtcg gccatgctcg
    10741 ccgagttgca gtcgcggctg ggcgagcgca tgggccgcga agtggtgctg atctcgctgt
    10801 cgatcgaccc gctcaccgac gacccgccgc gcatgcagtc cttcgcgaga cccttcgagc
    10861 ccgggcctca ctggtggtgg ctcaccggcg agccgcgcac gatgttcaag accctggagg
    10921 cgctcggcgc tgatcgcggc gacccgcgcg accacgcacc gttgtggctg gccggcactt
    10981 cgcagtcgcc caggcgcctc gtcggcctgc cgtccttgaa gcagctcgaa accgcgctcg
    11041 gtacaccgcc gagctcgccg cgatgaagac acctgacgga ccgctcgctc tgatgtctcg
    11101 ccgcgcgagc ctgcgctgtc tggctggctg cacgctcggc ttgaccgtcg gcgccgcggg
    11161 cgcgcaggcg cgcagcgaca aggcgcgcgc gtggttcacc gacaccacgc tgatcgatca
    11221 ggaccacagg ccgcatgcct tctactcgga cctgctggcg ccgcgtgccg tgctcatcaa
    11281 cgctgccttc gtcggctgtt cctcggcctg cccgttgctt acgcagcaac tggtcaaggt
    11341 acgcgagggg ctgggcgatc gtttcgggcg cgacatctgg ttcctctcca tcacagtcga
    11401 tccgctcagc gacgggcccg acgaactcaa gcgcttcgcg cagcggcagg gtgcggatgc
    11461 gcccggctgg cgtttcctga ctggcgcgcc gcaggacgtg aagcttgtgc tcggccgcct
    11521 cggtctgtgg gtcgagtcgc ccgacgccca ccaaaccggc ttgattgccg gtcgcgcagc
    11581 cggcgggcac tggggcaaac tgcgccccga tggcagctcg caggccctgg tccagcaact
    11641 ggcgcgcttt cttgcgccgg cgtagcagcg gcctgccgtg tcgcgtgctg gccatcgcct
    11701 tggtctttgg cgcgcttgcc gtgctggcac cggttcgagc ggcatcgacc gacggcggcc
    11761 gtgagctctt cgagcggggc accacgggcg gccagagcct gtccgcgcgg acgggcgctg
    11821 accgcagttg ggtgttgcgc ggcgcaaccg tggcctgtgc caactgccat ggtcttgccg
    11881 gcaccggagg cggcgagggc tacctgcgcg cgccggatct gcgctggccg cagtggtcgt
    11941 cgcccgaccc gcgccagcac gccgccgccc gcgaacgctt gcgtcgagct ctgcgccagg
    12001 gcatcggcgg tgacggtcgc actctcggtg ccgcgatgcc gcgtttcgat cttgacgacg
    12061 ccagcctcga cgccctggcc ggccatctgc agcgactggc caccgaacgg acgccgggca
    12121 gcaggccacg cctggccgtg atgcaactcg acgacgggca gcagggaccg accgagagcg
    12181 aactcggacc ggcgctccgg gcttgcctgc gccagcggct gggcgatcga gccgaagtgg
    12241 agttcgtcat ggtcgccagt ccggccgagg cgcaagcgca atggcgccag tggcagcaac
    12301 gttccgaagt gatggccgtg ctggcgccgc cctggcgggg ctggcggcca cccgcgcctg
    12361 ccgagggtac ggtcgcgctg gcggcgctct ttccgctcgt ggctgacccg gaaactggcc
    12421 gcgtgcagag cgtgcagtgg ttgttcggcg gcgtcgaagc gcgcgccgtg gcgctggtcc
    12481 aggcttggct gcagcaacct caggcccagt cggccacctt gcctgtttgg gtgggagagg
    12541 gcgctcttgc gccggcggcg cctgagatgc tcgagcgcat ggcacaggga gtgaggcgcg
    12601 ataccggccg gacgctggcc tggcaggtgc tggactcgcc ccggctgcgc actggcgaag
    12661 ttggtctctg gctcgatgcg cagcgaccgc ctggcgacgg ctggtggttg ctgccacatg
    12721 gggcgacagc ttcgcccggg cccgcggcgc gttggtgggt ggctgcgccg tttgccggcc
    12781 atccgccgcg atcactcgcc caacgttggg ccgatgccac ctgccgcacg gctcaggccg
    12841 cgatcgccga ccagcccgtc ttgtcgcgtg agcgctggct gccggcggtc gcgaacaccg
    12901 ctcggctgcg cgacggcgct gggtgggagt ggcatgttcc ggcgcacgat gagtatggct
    12961 acggagcatc caccgcgtgg accatcgtgg agatcgcggg cgggactgag cccacgccag
    13021 tggcgcccca ggtcaacatc gggcggccga tcgaggcggc acggtgaggc gtagcgccct
    13081 ccaagcgtca gccgctggcg gacaggaagc ccttcagttg atcgcgcatc gccctgcgcc
    13141 ccgcgccgtt gctcgcacgc caggcggcca gcgccaattc gaggaacgcc gcttctgcgg
    13201 ctgtcggagc ggcatccttg cgcagttcgt cgagccaacc cgatggctta ccgctggccg
    13261 actcgatctg ccgcgccagc ttgtcgccga tcgggcgaga cgacttgatc tggctccaca
    13321 tgctcggaga gatctgcagc gtggaggcga acgattgctc gagccccttg ggcgagccac
    13381 ctgaagccaa tgccttctcg gcgtagtcct ggaacagcgc cagcgcgttc tgtcgtctcg
    13441 tgagggtgat gttcgacacg tgtgtgtgcg caatgcaacg aagtcagacg ccattctcgc
    13501 agggggttga cgagttgggt aaagagtctt tacagtccgc tcccatcgag aagccggctc
    13561 gcaagagcca ttgccagaac cacaggacat caccgtgaaa ttgaacaacg tcattcggac
    13621 ccaccgaccc ctgcaggggc tggtcgcgtt cgtcccgtt
//
LOCUS       DAOORV010000099        13579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5558_length_13579_cov_7.661343, whole genome shotgun sequence.
ACCESSION   DAOORV010000099 DAOORV010000000
VERSION     DAOORV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13579
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_13579_cov_7.661343"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..71)
                     /locus_tag="PKV56_12820"
     CDS             complement(<1..71)
                     /locus_tag="PKV56_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HOX68740.1"
                     /translation="MGWTQDQMAARAAQELEDGFYVNL"
     gene            complement(113..820)
                     /locus_tag="PKV56_12825"
     CDS             complement(113..820)
                     /locus_tag="PKV56_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOX68741.1"
                     /translation="MNKVYPSAVEALKGVVADGQLLAVGGFGLCGIPEALIAALRDSG
                     VKNLTVISNNAGVDGFGLGQLLETRQIKKMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFYTKTGVGTLVAEGKETREFDGAVYVMERALCPEVSLVKAWKA
                     DQSGNLLFRRTARNFNPAAAMAGRITIAEVEEIVPTGTFDPDSVHLPGIYVHRLVLNA
                     TPEKRIEKRTIRATAAA"
     gene            complement(977..1639)
                     /locus_tag="PKV56_12830"
     CDS             complement(977..1639)
                     /locus_tag="PKV56_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOX68742.1"
                     /translation="MRELSLLESRVLGVLVEKAHTVPDSYPLSLNSLVAGCNQKTARD
                     PVIQATEAEVQVAIDALKALHLAAESSGSRVTRYEHNMGRAMALPTQSVALLAVLMLR
                     GPQTVAELRASTERLHRFADLSAVEGFLNELSERPAEKGGPLTVKLPRAPGSRESRWA
                     QLLCGAVDMTAVAGESVCEDFISASELATLKCQQQEMRVEIESLRALVDRLYAELGVT
                     RN"
     gene            complement(1665..2447)
                     /locus_tag="PKV56_12835"
     CDS             complement(1665..2447)
                     /locus_tag="PKV56_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX68743.1"
                     /translation="MSYQIDLSGRVALVTGASSGLGTQFAKTLALAGAGVVLAGGNME
                     RLKTLRAEIEGDGGDAHVVALDVTDLDSIKSAVAHAETEMGTIDILVNNSGVSTSQKL
                     TEVTEDDYDFVFDTNTRGAFFVAQEVGKRMLARAKGSAPGTFTGGRIVNIASMAGLKV
                     LSKIGVYSMSKAAVIHMTRAMALEWGKHGINVNAICPGYIDTDMNHHEWLTDAGKRLI
                     ETLPRKRIGQPQDLDSVLMMLCANESHFINGAVIQADDGYAL"
     gene            2652..4337
                     /locus_tag="PKV56_12840"
     CDS             2652..4337
                     /locus_tag="PKV56_12840"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOX68744.1"
                     /translation="MTPTQLLEQYGPREAMEYDVVVVGGGPAGLAAAIRIRQLAQVQG
                     QEVSVVVLEKGSEPGAHILSGAVMDPRALDELLPRWKELGAPLEQPVTDDEFLFLTRD
                     GATKTPPALLPDCFHNRGNYVVSLGNVTRWLGQQAEALGVEIFPGFAAAEVLYNDDGS
                     VKGVATGNLGIGKDGEPTGNFQLGMELHARYTVFAEGSRGHLGRQLVSRFKLDAGRDP
                     QSYGLGIKELWEIAPEKHKPGRVVHTAGWPLDSQTYGGAFLYHLPNNQVTLGFITGLD
                     YSNPWLSPFEEMQRWKTHPAIRSVLEGGKRVGYGARAITAGGLLSLPKTVFPGGALVG
                     CDAGYLNVSRIKGSHAAIKSGMLAADAIFDAVTNGRRHDELSAYPEAFERSWLHEELD
                     RARNFKQWFKKGLWVASLMNGIEQWLLPKIGFKAPPWTLHHHVPDHQTLKPAAECPQI
                     AYPKPDGQLTFDRLSSVFLSNTNHEENQPAHLTLKDTGVPVGVNLAKYAGPESRYCPA
                     GVYEFVKNADNSDRLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYAGM"
     regulatory      4367..4475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      4489..4600
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4658..5809
                     /gene="gcvT"
                     /locus_tag="PKV56_12845"
     CDS             4658..5809
                     /gene="gcvT"
                     /locus_tag="PKV56_12845"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOX68745.1"
                     /translation="MSASSSATDLLKTPLHDLHLELGARMVPFAGYSMPVQYPAGLMS
                     EHLHCRASAALFDVSHMGQLRLTGSDAAAALESMLPIDVAGMADGQQRYGFLTNDHGG
                     ILDDLMLTRHGVGDATEWLLIVNAANKEADTALLRSRIGDRCMVNPRPDLALLALQGP
                     QAVTALARLNPAVAALTFMSGAAVTLNGAACFATRSGYTGEDGFEISVPAPQAAALAR
                     ALLDQPEVKPAGLGARDTLRLEAGLCLHGNDIDTQTTPIEAGLAWAIQKVRRSGGARA
                     GGYPGSAVIDAQIAGGTARRRVGLVGLERAPVRAGTPIVDADGTPLGTVTSGTLAPTA
                     NRPIAIAYLPKNHLALDHEIYAEVRGKRLPMRVTALPFVPHGYVRAPAR"
     gene            5862..6236
                     /gene="gcvH"
                     /locus_tag="PKV56_12850"
     CDS             5862..6236
                     /gene="gcvH"
                     /locus_tag="PKV56_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOX68746.1"
                     /translation="MTILYTAEHEWIDATDPTAAIVGITVHAQDALGDVVFVDLPEVG
                     AIFAQGDVAGVVESVKAAADLYMPVSGEVIEVNEALRADPALANSDPLASGWFFKVKV
                     SDASQLGALMDSVAYDTLLKGL"
     gene            6371..9325
                     /gene="gcvP"
                     /locus_tag="PKV56_12855"
     CDS             6371..9325
                     /gene="gcvP"
                     /locus_tag="PKV56_12855"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOX68747.1"
                     /translation="MLMSTPPQPSPTTLAELENPTEFAARHIAITEADEQHMLSVITP
                     GQPNFTRRALIEAIVPRSIVRTRPMSLPSPVGEAQALAEMKAIAAKNQVLRSFIGQGY
                     HGTHTPGVILRNILENPAWYTAYTPYQAEISQGRMEALVNFQTMVCDLTGMAIANASM
                     LDEATAAAEAMTLARRSVKSKSDTFIVAGDCHPQTIEVIQTRAAPLGLKVVLANSAAE
                     WDALISGGDYFAALEQLPSTTGALHDLRGDAERIHAHQAAFIVAADLLALTLITPPGE
                     FGADIALGTTQRFGVPMGAGGPHAAYLACRDEFKRAMPGRLVGVSVDAHGAPALRLAL
                     QTREQHIRREKATSNICTAQVLLAVIASMYAVYHGPHGLQRIAQRVARYTAVLAQGLR
                     DLGCHVRSTSAFDTLSLDTGTETALLAERARAHGINLRRYPQWGDTMLGIALDETTTR
                     DDIATLWQVFAPAGRALPSEQLFDQPNDTMIPPELRRTSAFLTHPVFNSHHSETEMLR
                     YIRSLSDKDLALDRTMIPLGSCTMKLNATSEMIPITWPEFANMHPFAPADQLEGYRLL
                     DEQLRAALCQATGYSGISLQPNAGSQGEYAGLLVIHAWHVSRGEGHRDICLIPESAHG
                     TNPASAQMAGMKVVVTSCDARGNVDLDDLRAKCELHSANLAAVMVTYPSTYGVFDPQI
                     RQLCALVHQHGGRVYVDGANMNALVGIAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVG
                     PVCVASDLVPFLPGHRTAGPGDARQVGAISAAPLGNAAVLPISWMYMRMMGAAGLTAA
                     TETAILSANYIAARLADHYDIRFSGGVAGVRGGGIAHECILDLRPLKETSGITAEDVA
                     KRLIDYGFHAPTLSFPVPGTLMVEPTESEPLRELDRFCDAMIAIRAEIRKVETGAWPQ
                     HANPLKAAPHTAEALLAADWPHPYTREEAAFPVASLRRSKYWVPVGRVDNVHGDRNLV
                     CSCPPLSEYADG"
     gene            9344..10768
                     /locus_tag="PKV56_12860"
     CDS             9344..10768
                     /locus_tag="PKV56_12860"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOX68748.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFVHRLIADAQLAGTR
                     RVKAELFGSLGATGRGHGSDKAVLLGLAGHEPDTVDVEAIPALLAAIRDGRRLPLGSD
                     HAVAFDEKADLVFHRRQSLPLHANGMRFTAYGDDGNGIVERVYYSVGGGFVMSDEVLA
                     DGTRQKQIAPDTTVLPFPFHSGADLLAITTREGCSIAEVMRRNERHWRSDAELDAGLM
                     KIWDVMQACVVRGCATEGVLPGGFKVRRRAPDLHRQLAAESDPSHPGPLSALRFDPAD
                     PLQVLDWVNLYALAVNEENAAGGRVVTAPTNGAAGIVPAVLHYYRRFIPGASDRGVID
                     FLLTAAAIGLLYKENASISGAEVGCQGEVGVACSMAAGALCAVLGGTPEQVENAAEIG
                     MEHHLGLTCDPVGGLVQIPCIERNAIASVKAINAARMALRGDGTHHVSLDKVIKTMRD
                     TGADMMTKYKETARGGLAVNIVEC"
     gene            11237..11524
                     /locus_tag="PKV56_12865"
     CDS             11237..11524
                     /locus_tag="PKV56_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68749.1"
                     /translation="MVLDFAVCAGLGVAAVSCAIAAAVAVRASTTGATTKATSAAVGI
                     LAFVVFGGALLAIAAILGETKASRDPNSTLGFVVGASVVSIAFYAAVRRRT"
     gene            complement(12118..12378)
                     /locus_tag="PKV56_12870"
     CDS             complement(12118..12378)
                     /locus_tag="PKV56_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68750.1"
                     /translation="MPFNVGVQPPPKVVGWNNGLACIRTLNISDVPDKFSEVALQDQP
                     LAALNPGLNSITAEPGGAISLPFNQADLMHATETLSRADGSL"
     gene            12721..13470
                     /locus_tag="PKV56_12875"
     CDS             12721..13470
                     /locus_tag="PKV56_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68751.1"
                     /translation="MPHSSRTDSPTDVWATTFGSVVVGSILVLLLFTAFAGWPWVAKF
                     LESSAPAWIQAVGSVAAIVAALAVVQRQHSLELNRREKEDLTAQLRRARSLRVLFYSA
                     ARTCEDVARRIGRPHQTWKFLAEELREVRGRLLAVDPLLVPEGGLLLIIEECAMRLQS
                     CALIVAELERPRKKETEDGIRFAVMATARECWLGLYEATGLEAKLCQSGTADEQPYPF
                     DDFDASRKKLDEIRANFVRDRQDQAADEGPR"
BASE COUNT         2364 a         4593 c         4308 g         2314 t
ORIGIN      
        1 aggttgacat agaagccgtc ttccagctcc tgcgcggcgc gtgccgccat ttggtcttgt
       61 gtccagccca tggtgcgtgt gctcctgtcc tgtggtgaag gggtgcggcg cctcaggcgg
      121 ccgccgtggc gcggatggtg cgcttctcga tgcgcttctc gggcgtggcg ttcagcacca
      181 ggcggtgcac atagatgccg ggcaggtgca ccgaatccgg gtcgaatgtg ccggtcggca
      241 cgatctcctc gacctccgcg atggtgatgc gacccgccat cgcggcggcc gggttgaagt
      301 tgcgcgcggt gcggcgaaac agcaggttgc cgctctggtc ggccttccat gccttgacca
      361 ggctgacctc ggggcacagc gcgcgttcca tcacgtagac ggcgccgtcg aattcgcggg
      421 tctccttgcc ttcggccacc agggtgccga caccggtctt ggtatagaag gccgggatgc
      481 cggcgccacc agcgcgcagc ttctctgcga gcgtgccttg cggcgtgaat tccagctcca
      541 gctcgccggc cagatactgg cgctcgaatt ccttgttctc gccgacgtag ctcgaaatca
      601 tcttcttgat ctgccgggtc tcgagcagtt ggccaagacc gaagccgtcg acgcccgcgt
      661 tgttcgagat caccgtgagg ttcttgacgc cgctgtcgcg cagcgccgcg atcagcgctt
      721 cgggaatgcc gcaaagaccg aatccgccca ctgccagcaa ctgaccgtca gcgacgaccc
      781 ctttgagcgc ttccaccgcg ctcggataca ccttgttcat tgcgttcctc tccaggacct
      841 agggacgcgt tcaccagcac cccggcgacc gcaaacccag cattatgccc tcgcaaaccc
      901 tagggtttcc acttccacgc tcagggtaaa tactgctttt cggaccggtc agagccgccc
      961 gtgcgatcga tagcgttcag ttccgtgtca cgccgagctc ggcgtacagg cggtcgacca
     1021 gcgcacgcaa cgattcgatc tcgacgcgca tctcctgctg ctggcatttc agggtagcca
     1081 gttcgctggc agagatgaaa tcctcgcaga cgctttctcc ggccacagcc gtcatgtcga
     1141 cggcgccgca gagcaattgc gcccagcgcg actcgcgcga gccaggcgcc cgcggcagct
     1201 tgacggtcag cggcccgccc ttctcggccg gccgctcgga cagttcattc aggaagccct
     1261 cgacggcgga gagatcggcg aaccggtgca gccgttcggt gctggctcgc aactcggcca
     1321 cggtctgggg cccccgaagc atcagcaccg cgagcagcgc cacggactga gtcggcagcg
     1381 ccatcgcacg gcccatgttg tgttcatagc gcgtcacgcg cgaaccgctg ctctcggcgg
     1441 ccaggtgcag cgccttcaat gcgtcgatgg ccacctgcac ctcggcttcg gtggcctgta
     1501 tgacggggtc gcgtgcggtc ttctggttgc aaccggccac cagagagttc agcgacagcg
     1561 gatagctgtc cggaaccgtg tgagccttct cgaccagcac cccgagaacc cgcgactcga
     1621 gcagcgacaa ctccctcatc ggatgcaacg ccgcgctgtg cgaatcagag cgcgtaaccg
     1681 tcgtcggcct ggatcaccgc gccgttgatg aagtggctct cgttggcgca cagcatcatc
     1741 agaaccgaat ccagatcctg cggctggccg atgcgcttgc gcggcaaggt ctcgatcagg
     1801 cgcttgcccg cgtcggtgag ccactcgtgg tggttcatgt cggtatcgat gtagccgggg
     1861 cagatcgcat tgacgttgat gccgtgcttg ccccactcga gcgccatggc gcgcgtcatg
     1921 tggatcaccg ccgccttgct catgctgtag acgccgatct tgctcagcac cttcagcccg
     1981 gccatggaag cgatgttgac gatgcgccct ccggtgaagg ttccgggcgc cgagcccttg
     2041 gcacgagcga gcatccgctt gccgacttcc tgggcgacaa agaaggcgcc acgcgtgttg
     2101 gtgtcgaaaa cgaagtcgta atcgtcttcg gtcacctcgg tcagcttctg cgacgtgctg
     2161 acccccgagt tgttgaccag gatgtcgatc gttcccatct cggtctcagc atgcgccacg
     2221 gccgacttga tgctgtccag gtcggtgaca tcgagcgcca ccacatgtgc atcaccgccg
     2281 tcgccctcga tctctgcgcg aagtgtcttc aggcgttcca tgttgccgcc ggccagcacc
     2341 acgcccgcgc cggccagggc cagcgtcttt gcgaactggg tgcccaggcc actggaggcg
     2401 ccggtgacca gcgccacgcg gccggacagg tcgatttggt agctcatgtc tgtcaatccc
     2461 attctggaag ttcaggtgaa gatagcacgc tgactttgcg ctgcttgtcg catcagagcc
     2521 gtcgaatcgt cgctttccga gcttttcgac gcctgatgac gagcccggcg acgccggacc
     2581 cgcatcaggg ttcgacccga tgcctcccgc tagactccgg gagtgttttc gctgccgaga
     2641 aagtgcctca gatgaccccg acccagctcc tcgaacaata cggtccgcgc gaggccatgg
     2701 agtacgacgt ggtggtcgtc ggcggcgggc cggccgggct ggctgcggcc atacgcatca
     2761 ggcagctcgc ccaggtgcag ggccaggagg tctcggtcgt ggtgctcgag aagggatcgg
     2821 agcccggggc gcacatcctc agtggtgcgg tgatggaccc gcgagcactc gacgaactgc
     2881 tgcccaggtg gaaggagctt ggagcgccgc tcgaacaacc ggtcaccgat gacgagttcc
     2941 tgttcctgac gcgtgacggc gcgacgaaga ccccgccggc gctgctgccg gactgcttcc
     3001 acaaccgggg caactatgtc gtcagcctgg gcaacgtcac gcgctggctg ggccagcagg
     3061 ccgaagcgct gggcgtggag atatttcccg gcttcgcggc cgccgaagtc ctctacaacg
     3121 acgacggatc ggtgaagggc gtggccacag gcaacctcgg catcggcaag gacggcgagc
     3181 cgaccgggaa tttccagctc ggcatggagt tgcacgccag atacaccgtg ttcgccgaag
     3241 gctcgcgcgg gcacctgggc cgccaactgg tctcgcgctt caagctcgac gccgggcgcg
     3301 atccgcaaag ctacggactc ggcatcaagg agctctggga gatcgctccg gaaaagcaca
     3361 agccgggccg cgtcgtccac accgcgggct ggccgctgga cagccagacc tacggcggcg
     3421 ccttcctgta ccacctgccg aacaaccagg tcacgctcgg cttcatcacc ggcctcgact
     3481 acagcaaccc ctggctgagc ccgttcgagg agatgcagcg ctggaagacg caccctgcga
     3541 tccgcagcgt gctcgagggc ggcaagcgcg tgggctacgg cgcgcgcgcc atcaccgccg
     3601 gcggattgct gagcctgccg aagacggtgt ttcccggcgg cgcgctcgtc ggctgcgatg
     3661 ccggttacct gaacgtcagc cgcatcaagg gcagccacgc cgccatcaag agcggcatgc
     3721 tggccgccga tgcgatattc gacgcggtga ccaacggccg ccggcacgac gaactgagcg
     3781 cctaccccga ggccttcgaa cgcagctggc tgcacgagga actcgaccgc gcgcgcaatt
     3841 tcaagcagtg gttcaagaag ggcctgtggg tcgcgagcct gatgaacggc atcgagcagt
     3901 ggctgctgcc gaagatcggt ttcaaggcgc ccccgtggac gctgcaccat cacgtgcctg
     3961 accaccagac gctgaaacct gcggcggaat gcccgcagat cgcctacccc aaacccgatg
     4021 ggcagctgac gttcgaccgt ctgagcagcg tcttcctcag caacaccaat cacgaggaaa
     4081 accagccggc tcacctgacg ctgaaggaca ccggcgtgcc tgtcggcgtc aacctcgcga
     4141 agtacgccgg cccggagagc cgctactgtc cggcaggtgt ctacgagttc gtcaagaacg
     4201 ccgacaacag cgatcgcctt cagatcaacg cgcagaactg cgttcactgc aagacctgcg
     4261 acatcaagga tccgacgcag aacatcgtgt gggtcacgcc cgagggcggc ggcgggccca
     4321 actacgcggg catgtagagc aggcttgcga aatcgctgcg atacactcgc cgccgtcagg
     4381 agagagcccg gttgattcga tcgggccgcc gaaggcgcaa ggccacccgg gaacgggtcg
     4441 ctccaacgct caggcaaaag gactggcaac acgccgaccg tttggacggc gttcctcgct
     4501 gaagagaggc ccaccccgag tgggtccacc gaaggggcaa gcgtttcacc gcgttgtggt
     4561 gagcgtcaat ctctcaggta aagcggacag agagggcatc ggcggctgtg catccatcgg
     4621 atgcgcggct ggttgtcatg gccccctgga aatgttgatg tccgcctcct cgtccgcaac
     4681 cgatctgctc aaaactccgc tgcacgacct gcacctcgaa ctgggtgcac gcatggtgcc
     4741 cttcgccggc tacagcatgc cggtgcagta ccccgccggc ctgatgtccg aacacctgca
     4801 ctgccgcgcc tcggccgcgc tgttcgacgt gtcgcacatg gggcagctga ggctgaccgg
     4861 ctccgatgcc gccgcggcgc ttgaatcgat gctgccgatc gacgtcgcgg gcatggcaga
     4921 cggccagcag cgctacggct tcctgaccaa cgaccacggc ggcatcctcg acgacctgat
     4981 gctgacgcgc cacggcgtgg gtgacgccac cgaatggctg ctgatcgtca acgcggccaa
     5041 caaggaggcc gacacagcgc tgctgcgctc gcgcatcggt gatcgctgca tggtgaatcc
     5101 gcgccccgat ctggcactgc tggcgctgca gggcccgcag gcagtgactg cactcgcgcg
     5161 actgaatcca gccgtcgcgg ccctgacctt catgagcgga gcggccgtga ccttgaacgg
     5221 agccgcctgc ttcgccaccc gatccggcta caccggcgag gacgggttcg agatctcggt
     5281 gcccgcgccc caggccgcag cgctggcgcg cgccctgctg gaccaaccag aagtgaagcc
     5341 cgccggcctg ggcgcgcgcg acacgctgcg gctcgaagcg ggtctgtgcc tgcacggcaa
     5401 cgacatcgac acgcagacca ccccgatcga ggccggtctg gcctgggcga tccagaaggt
     5461 gcgccgatcc ggtggcgcgc gcgccggcgg ctacccgggt tcggcggtca tcgatgcaca
     5521 gatcgcaggc ggcaccgcgc gccgccgcgt cggcctggta ggactggaac gtgccccggt
     5581 gcgggccggc acccccatcg tcgacgcgga cggcacaccg ctgggcaccg tcaccagcgg
     5641 cacgctggca ccaaccgcga accgcccgat cgcgatcgcc tacctgccga agaatcacct
     5701 cgcgctcgat cacgagatct acgccgaagt gcgcggcaag cgcctgccga tgcgcgtcac
     5761 ggcgctgccc ttcgtgccgc acggctatgt cagggcgccc gcgcgctgaa ggcctgcgcc
     5821 ctgcttgtct cccaacccaa cacctgcaac cggagtcccc gatgaccatc ctctacaccg
     5881 ccgaacacga gtggatcgac gccaccgacc cgaccgccgc gatcgtcggc atcaccgtgc
     5941 atgcacagga cgcgctgggt gacgtcgtgt tcgttgacct gcccgaagtc ggcgccattt
     6001 tcgcccaggg cgacgtcgcc ggcgtcgtcg aatcggtcaa ggccgcggcg gacctgtaca
     6061 tgccggtcag cggcgaggtg atcgaagtca acgaagcgct gcgcgccgac ccggcgctgg
     6121 ccaacagcga tccgctggcg agcggctggt tcttcaaggt caaggtgtcc gatgcatcgc
     6181 aactcggcgc cctgatggac agcgtggcct acgacacgct gctcaagggc ctctgattct
     6241 tcgttcaatt cataccgctc attccacgca cccctgcccc gcggggactg cacctccgta
     6301 catggcgact cgattccggc tgcggtgagc tcaccgcgcc ttcgaggttc gccccaaaaa
     6361 ggtcgcaacc atgttgatgt ccaccccacc ccaaccctcg cccaccacac tggccgagct
     6421 agagaacccc accgaattcg ccgcgcgcca cattgcaatc accgaagctg acgaacagca
     6481 catgctgtcg gtgatcaccc ccggccagcc gaacttcacg cgccgcgcgc tgatcgaagc
     6541 gatcgttccg cgttcgatcg tgcgcacccg accgatgtca ctgccatcgc cggtcggcga
     6601 ggcacaggcg ctggccgaga tgaaggcgat cgccgcgaag aaccaggttc tcaggagctt
     6661 catcggccag ggctatcacg gtacgcacac gccgggcgtc atcctgcgca acatcctcga
     6721 gaaccccgcc tggtacaccg cctacacgcc ctaccaggcc gagatctcgc agggccgcat
     6781 ggaggcgctg gtcaacttcc agaccatggt ctgcgacctg accggcatgg ccatcgccaa
     6841 tgcgtcgatg ctcgacgagg ccaccgcggc cgccgaagcg atgacgctcg ccaggcgcag
     6901 cgtcaagtcg aagagcgaca ccttcatcgt ggccggtgac tgccacccgc agaccatcga
     6961 ggtgatccag acccgcgctg cgccgctggg actgaaagtg gtgctggcca actcggcagc
     7021 cgaatgggat gcgctgatca gtggcggcga ctatttcgcc gcgctggagc aactgcccag
     7081 cacgaccggc gcgctgcacg acctgcgcgg cgacgccgaa cgcatccacg cgcatcaggc
     7141 ggccttcatc gtcgcagccg acctgctggc gttgacgctg atcacgccac cgggcgagtt
     7201 cggcgccgac atcgcgctcg gcaccacgca gcgcttcggc gtgccgatgg gtgcgggcgg
     7261 tccgcatgcg gcgtacctcg cgtgccgcga cgagttcaag cgcgcgatgc cgggacggct
     7321 ggtcggcgtc agcgtcgatg cgcacggcgc accggcgttg cggctcgcgc tgcaaacgcg
     7381 cgagcagcac atccgccgcg agaaggccac gtcgaacatc tgtaccgcgc aggtgctgct
     7441 ggccgtcatc gcgagcatgt atgccgtgta tcacgggccg cacggactgc agcgcatcgc
     7501 gcagcgcgtg gcccgctaca ccgcggtgct ggcgcagggc ctgcgggacc tgggctgcca
     7561 cgtgcgttcg accagcgcat tcgacacgct gtcgctcgac accggcaccg aaacggcgct
     7621 cctcgccgag cgcgctcgcg cccacggcat caacctgcga cgctacccgc agtggggcga
     7681 cacgatgctg ggcatcgcgc tcgatgaaac gacgacgcgc gacgacatcg cgacgctctg
     7741 gcaggtgttt gctccggccg gccgcgcgct gccgtccgag cagctgttcg accagccgaa
     7801 cgacacgatg atcccgccgg aattgcgccg caccagtgca ttcctgacgc acccggtgtt
     7861 caacagccac cacagcgaga ccgagatgct gcgctacatc cgcagcctgt ccgacaagga
     7921 cctggcactc gaccgcacga tgatcccgct gggctcgtgc acgatgaagc tcaacgcgac
     7981 gagcgagatg atcccgatca cctggcccga gttcgcgaac atgcatccgt tcgcgccggc
     8041 cgatcagctc gaggggtacc gcctgctcga cgagcagttg cgcgccgcgc tgtgccaggc
     8101 caccggctac agcggcatca gcctccaacc caatgccgga tcgcagggcg aatacgccgg
     8161 gttgctggtc attcacgcgt ggcacgtgtc gcgcggtgaa ggacaccgcg acatctgcct
     8221 gatccccgaa tcggcgcacg gcaccaaccc ggcgtcggcg cagatggccg gcatgaaggt
     8281 cgtcgtcacg agctgcgacg cccggggcaa cgtcgacctc gacgacctgc gcgccaagtg
     8341 cgaactgcac agcgcgaacc tggccgccgt gatggtcacc tatccgtcga cctacggcgt
     8401 gttcgatccg cagatcaggc agctgtgcgc gctcgtgcac cagcatggcg ggcgtgtcta
     8461 cgtggacggg gccaacatga acgcgctggt cggcatcgcg gcgccgggcg agttcggcgg
     8521 cgatgtcagc cacctgaatc tgcacaagac cttctgcatc ccccatggcg gcggtgggcc
     8581 gggcgtcggc ccggtctgcg tcgcctccga tctggtgccc ttcctgcctg gccaccgcac
     8641 cgcaggcccg ggagacgcgc gccaggtcgg cgcgatcagc gcggcaccgc tgggcaatgc
     8701 cgcggtgctt ccgatcagct ggatgtacat gcggatgatg ggcgccgccg gactgactgc
     8761 cgcgaccgag acggccatcc tgagcgccaa ctacatcgcc gcgcggctgg ccgatcacta
     8821 cgacatccgt ttcagcggcg gcgttgcggg cgtccggggc ggcggcatcg cgcacgaatg
     8881 catcctggat ctgcggcccc tcaaggagac cagcggcatc accgccgagg acgtcgcgaa
     8941 gcggctgatc gactacggct tccacgcgcc gacgctcagc tttccggtgc cgggcacgct
     9001 gatggtggag ccgaccgaaa gcgagccgtt gcgcgagctc gaccggttct gcgacgcgat
     9061 gatcgcgatc cgggccgaga tccgaaaggt cgagaccggc gcgtggccgc agcacgcaaa
     9121 cccgctgaag gccgcgccgc acaccgccga agcgttgctc gcggcagact ggccgcaccc
     9181 ttacacccgc gaggaagctg cattccccgt ggccagcctg cgccgcagca agtactgggt
     9241 gccggtgggt cgcgtcgaca acgtgcatgg cgacagaaac ctcgtgtgca gctgcccgcc
     9301 gctgtcggag tacgctgacg gctgaccctg gaaggagcgc cccatggccg tgtcggtttt
     9361 cgaccttttc aagatcggca taggccccag cagctcgcac accgtcgggc cgatgcgcgc
     9421 agcgcgtctg ttcgttcacc gcctgatcgc cgacgctcaa ctggccggca cccgacgtgt
     9481 caaggccgag ctgttcggct cactcggtgc caccgggcgt ggccacggca gcgacaaggc
     9541 ggtgctgctg gggctggccg gacacgagcc cgacacggtc gatgtggaag ccattccggc
     9601 gctgctggcc gccatccgcg acggccgccg gctgcctctg ggcagcgacc acgcggtggc
     9661 gttcgacgag aaggccgacc tggtattcca ccgccgtcag agcctgccgc tgcatgccaa
     9721 cggcatgcgc ttcacagcct acggcgacga tggaaacgga atcgtcgagc gtgtctacta
     9781 ctcggtcggc ggcggcttcg tcatgagcga cgaggtgctc gccgatggca cccggcagaa
     9841 acagatcgcg cccgacacca ccgtcctgcc cttcccgttc cattcgggtg cggacctgct
     9901 ggcgatcaca acccgcgagg gctgcagcat cgccgaggtg atgcgccgca acgagcggca
     9961 ctggcgcagc gatgccgaac tcgacgccgg gctgatgaag atctgggacg tgatgcaggc
    10021 ctgcgtggtg cgcggctgcg ccaccgaggg cgtgctgccg ggcggcttca aggtgcggcg
    10081 ccgtgcgccc gacctgcacc gccagttggc ggccgagtcc gaccccagcc acccgggccc
    10141 gctcagcgcg ttgcgcttcg atcccgccga cccgctgcag gtgctggact gggtcaacct
    10201 gtacgcgctg gcggtcaacg aggaaaacgc cgctggcggc cgcgtggtca ccgcaccgac
    10261 caacggtgcc gcgggcatcg tgccggcggt gctgcactac taccgccgat tcatccccgg
    10321 cgccagcgac cggggcgtga tcgacttcct gctgaccgct gcagcgatcg gcctgctcta
    10381 caaggaaaac gcatcgatca gcggcgccga ggtcggctgc cagggcgaag tcggcgtcgc
    10441 ctgctcgatg gccgccggtg cgctgtgcgc ggtgctcggc ggcacgcccg agcaggtcga
    10501 gaacgccgcc gagatcggca tggagcacca cctcggcctg acctgcgacc ccgtcggcgg
    10561 gctggtgcag atcccctgca tcgagcgcaa cgcgatcgcg tccgtcaagg ccatcaacgc
    10621 ggcccgcatg gcgctgcgcg gcgacggcac acaccacgtg agcctggaca aggtcatcaa
    10681 gaccatgcgc gacaccggcg ccgacatgat gaccaagtac aaggaaacgg cgcgcggcgg
    10741 gctggcggtg aacatcgtgg agtgctgatg ctgccgagca gcgcaagcag cttggccgca
    10801 gcgaatcggg ctgcgagtgc tatcgaactg actggcgctg atcgacccat tgcggtcgca
    10861 tgtctgcggg ccaccactga ccgtctggtc cagcaaagct gccggactca ctgctgaagc
    10921 cagtcgacct ctcgttgcag ccagacagtt ttgaccgatt tcaggtgtac ttccgactgg
    10981 catcatctag ccatcgtcgg gccggcctcc tagtggcgag aacttgcaac tgcacgccgc
    11041 ttttgccgga aatttaaagc ctgtttcgcc cgtcaatttt tcaagaaagt tccaatgagt
    11101 caagcacttg cgatgaccca acagggatgt gttgtagcgc aacatgcctg cctaagcagc
    11161 atcggctgtc tccccagaac acgttaggcc tcgcaagcac tacgcacggc tcgcatacat
    11221 gtatcttggt cagtcggtgg ttctcgattt cgcggtgtgt gccggcctgg gcgtggctgc
    11281 cgtgtcgtgt gcgattgcgg cagccgttgc agtccgcgcg agcacgacgg gtgcaacaac
    11341 caaggcgacg tccgcagcgg tcggcatcct tgccttcgtc gtctttggag gagccttgct
    11401 cgcaatcgcg gcgattcttg gcgaaacaaa ggcgtctcgt gacccgaact ccacccttgg
    11461 gttcgtggtg ggggcctcgg tcgtgtcgat tgctttctac gctgcggttc gccgtcgaac
    11521 ctgaggctgc gagaagcaag ccagtccgac tctcgccaag gacagactcc accccgaaca
    11581 ccgagtgaat agccggcacc cgacagcaag gcctaaccct tcgctcgagc cgacccgctc
    11641 cggcaaggcg ccctggcctc caagatcccc gtactatctc gttctcgtcg gccagggcgc
    11701 cttgcctcca cggtcggctc agctcaaacg ttagcccgca tgagtaccct ctcgccatcg
    11761 acacgagccg aagtagcaag agctggctgg atcatgctgc cgctgccatc tctcccgcta
    11821 atcttgcttc tcgtattttg gtggcgcgtg tacttctcta ctgatgcgca ggtttacggc
    11881 gctgtcgtat ccgcagtctt gtgcgtccct gctacacgcc tattgttccg tcgcgcgaga
    11941 aggctggcat cgctcggaat tcatgcgtac tacccgcgag gtgaggctat accgttaaag
    12001 caacaacttc tcgctggcat tgggcttttc gtgctcttgt acctttgggc actcacgcta
    12061 gtcgcactcg cgttgcccgt ttccggcgcc gtcgcaacgg agcgatcctt cgtcgtctca
    12121 gaggcttccg tctgcacgag aaagtgtctc ggttgcgtgc atcaggtcgg cttggttgaa
    12181 tggcaaggaa atagcgccac caggttctgc agtgatgctg ttcagccccg ggttcaaagc
    12241 ggcgagcggc tggtcctgca aggctacttc actaaacttg tctggtacgt ccgagatgtt
    12301 cagcgtgcga atgcaggcta acccgttgtt ccagccgacc accttcggcg gcggctgaac
    12361 gccaacgtta aagggcatcc atatgaaccc tcaggtgggc gtgaacatct tggtcatgtt
    12421 gtctggcctt gccatagtgg cagtgctttt cttccttggc cgtcgcatgg tgtctgagca
    12481 acatccttgg agaaggtggg caactggtgt tttcaagcgc aatgggctgg ttcaaacggc
    12541 gcgcctggct ggtctggtgt tgttagcggt cgtccttagt gcgcttaccc ttctgtactc
    12601 gtagccatgc gcagattccc tttaacaggc cgctccatcc gaccgccttc ggcgccggct
    12661 gagcggcggc gttaggcgtc gcaaccaaat cgcctgccac gtcaacgcaa gactgatccg
    12721 atgccgcact cctctcgcac tgactccccg accgatgtgt gggccaccac gtttggcagc
    12781 gttgtggtgg gctcgattct tgtcttgctc ctgttcactg catttgcagg atggccctgg
    12841 gtagccaagt ttctcgagtc aagtgcaccg gcctggattc aagccgttgg ctccgtcgcc
    12901 gcaatagtcg ccgcgctggc ggtggttcag cggcagcact cgttggagtt gaatcgaagg
    12961 gagaaggagg atctcaccgc gcagttgcgc cgtgcgcgtt cactcagagt tctcttctac
    13021 tccgccgcgc ggacctgcga ggatgtcgcc cgacggatcg gcagaccaca tcagacctgg
    13081 aagttcctgg ccgaggaact ccgcgaagtc cgcggaagac tgcttgctgt cgacccgctt
    13141 cttgtgccag agggcggcct cttactcatc attgaagaat gcgcgatgcg tctccagagt
    13201 tgcgctctga tcgttgccga actcgagaga ccgcgaaaga aggaaacaga ggatgggatc
    13261 aggtttgccg taatggctac tgctcgcgag tgttggctcg gactgtacga ggccactggg
    13321 ctggaggcca agctctgcca gtctggcacc gcggacgaac agccgtatcc gttcgacgac
    13381 ttcgatgcaa gccgaaagaa gctagatgag attcgcgcga acttcgtacg agaccgtcag
    13441 gaccaagcag cggacgaagg gccgcgttag cggcgcagcg acgcctaact cgaacgttag
    13501 gcgacgtgac acgcgtcaag agacctgcga ggtaggagga acaacgtgcc accgttcgac
    13561 ggcttcgatc tggagtcct
//
LOCUS       DAOORV010000100        13548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5579_length_13548_cov_9.281331, whole genome shotgun sequence.
ACCESSION   DAOORV010000100 DAOORV010000000
VERSION     DAOORV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5579_length_13548_cov_9.281331"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..480)
                     /locus_tag="PKV56_12880"
     CDS             complement(40..480)
                     /locus_tag="PKV56_12880"
                     /inference="COORDINATES: protein motif:HMM:NF026932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm50 family immunity protein"
                     /protein_id="HOX68752.1"
                     /translation="MEPHAFIEGHEEVLARFGQWPSFHDGEIHRLLLDSTRRDADGRR
                     YPSIEVDLRGWIMTIEVTEQGFYRCEFDSVVRFLFEHVSSVELDGLNHQNALSCLDLE
                     LLAPEAGSSQLRVELEHCFGLSGSFVAGRASVVSVTPYVAASAA"
     gene            complement(540..1067)
                     /locus_tag="PKV56_12885"
     CDS             complement(540..1067)
                     /locus_tag="PKV56_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOX68753.1"
                     /translation="MSATFTLTVADFARLQKVVAKRFRSKAGLFSLQFGLRVTVWLCI
                     GLAGATYARVMREYPEISQPLGTVAVLLVLALLAIVALPYVAQVSLRKFMLAPNGAFL
                     SPQTVSLSAASVHVNSSTGTTEVPWSGVLFRAEDEANYYLFIDGMQALVLPRAAMAPF
                     VAELEQYTAHIKNAT"
     gene            1712..1873
                     /locus_tag="PKV56_12890"
                     /pseudo
     CDS             1712..1873
                     /locus_tag="PKV56_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2070..3815)
                     /gene="nifA"
                     /locus_tag="PKV56_12895"
     CDS             complement(2070..3815)
                     /gene="nifA"
                     /locus_tag="PKV56_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HOX68754.1"
                     /translation="MIRPSQQERSHIELIAVYEICRVLGASLDLQRSFRAALNILDAQ
                     IGLPRVMIVLPDEDDSRLRVHSYVGLDREQAGRGVWQVGEGMIGRVYQHGVSVTVPDV
                     TQAEEFIDRTGAFSAQDGQMMSFMVVPIKTDHEVLGVLAAQRAVEGNARLSDDHRILK
                     MVATLLAQTIVLSRAVAAEHQRLQLETTRLQKALAPEVRGRYALDNVVGVSAEMQQVF
                     AEVHQAAPSRATVLLRGESGTGKEAIARAIHYLSPRKDAPFIKVNCAALTESLLESEL
                     FGHERGAFTGAVGDRKGRFEQAHTGTLFLDEIGDVSPSFQAKLLRVLQEREFERVGGN
                     RSIKVDVRLICATNRDLEKMVKRGEYRADLYYRINVVSVFLPPLRERRADIPPLVAHF
                     VDRYNKENRRKLKIAGDAMEVLTHCYWPGNVRELENCIERTATMVQGDVIRGIAFPCR
                     QNRCLTQTLHHLDKADAVSPVQMSTPLHIPVVRSSVKDLPRPVRDSAESDENWSEAEM
                     DAADDVLRIGPTEHLNGTPRQFGDEPPEGERERLIWAMEQCAWVQAKAARLLKVTPRQ
                     LGYALRKYSIEVHKF"
     gene            complement(4076..4399)
                     /locus_tag="PKV56_12900"
     CDS             complement(4076..4399)
                     /locus_tag="PKV56_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOX68755.1"
                     /translation="MPKPQRHVFVCNQQRPPNHPRGSCASAGAGPLLQAFWAEQQKRQ
                     AYDKIAVTYSGCLGPCDGGPNVLVYPENVLYRGVAAADVPEIFDKHLEGGEVVERLRA
                     DPAVW"
     gene            complement(4516..5055)
                     /locus_tag="PKV56_12905"
     CDS             complement(4516..5055)
                     /locus_tag="PKV56_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOX68756.1"
                     /translation="MNGVLTKKGRVAVAAMFELAISSESAPVALAVVGERQQISLSYL
                     EQLFGRLRRHELVQSTRGPSGGYVLARDSDSITLADIISAVDDPGSAGARGRDSEPAE
                     ESSCLPVTQDLWDGANAKLAEYFDSISLKSLVDDRIAKGLVAKPASLKRGISPRRVLK
                     PIHVSKANWVFALAEAPAR"
     gene            complement(5114..5410)
                     /locus_tag="PKV56_12910"
     CDS             complement(5114..5410)
                     /locus_tag="PKV56_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOX68757.1"
                     /translation="MSAAVNVEDKLFQNRYKVDHGRPHIQIKDAAICANDCAEQQCTY
                     VCPAACYTAEGNGAVTLITDGCLECGSCRVICTEHTNVAWEYPRGGHGILFKFG"
     gene            complement(5407..6711)
                     /locus_tag="PKV56_12915"
     CDS             complement(5407..6711)
                     /locus_tag="PKV56_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOX68758.1"
                     /translation="MAEQFDAIVVGAGPSGNAATYTLAKAGLKVLQIERGEYPGSKNV
                     QGAILYAQALEQIIPDFRDDAPLERHIIEQRMWMLDDNSFVGTHYRSDDYNKPPYNRY
                     TIIRAQFDKWFSSKVREAGALLICETTVEHLLLDGDTVVGVHCDRMGGEVRADVVILA
                     DGVNSTLARKAGFHGEISSKNVALAVKEILFMPEETIQSRFNIKEDEGVVIELMGTVT
                     EGMVGTGFLYTNKDSLTIGVGCMLSDFKANPKKTTPYALLEKLKRHPSVAPLIEGGEM
                     KEYAAHLIPEGGFNAVPKIFGNGWMIVGDSGGFVNAAHREGSNLAMTTGRLAAETVIA
                     ARAAGRPMTARTLKAYKDALDESFVMKDLKKYRNLPDVLEKSPQFFTTYPELVNRAAK
                     TLFTVDGVDKMTKQREITGSFKSARKFTGLVGDAIKLWRATR"
     gene            complement(6723..7823)
                     /locus_tag="PKV56_12920"
     CDS             complement(6723..7823)
                     /locus_tag="PKV56_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX68759.1"
                     /translation="MSEAPKPAAPKAAGRAGRNTELSEALKAYKGVWVFIEHDRGHVN
                     PVSWELMGEARKLADKLQVDVAGVLMGGPDEPLDAYAAEAYAYGADRCYFVRDPVLKG
                     YRNEPFTKGLTDLVNKHQPEIMLLGATTMGRDLAGSVATTLGTGLTADCTELNIDGRA
                     LAATRPTFGGSLLCTIMTLAYRPQMATIRPRVMQMPRRDASRTGDGVEMPLGMIETDV
                     VTKLLDFIPDANRNTVNLAYADVIVTGGKGLKQPDNFKLIWDLARVLGAEVGGTRPVV
                     QAGWIEADRQVGQTGKTVRPKLYIAAGVSGAIQHRVGMESSDVIVAINTDPNAPIFEF
                     AHYGIVGNAMQVLPVLTEAFRTHLDKRIRKVA"
     gene            complement(7833..8681)
                     /locus_tag="PKV56_12925"
     CDS             complement(7833..8681)
                     /locus_tag="PKV56_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX68760.1"
                     /translation="MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIVNPYDLFALEE
                     ALRLKDRFGGRVTMLCMGPPQAEDALRKCISFGATDAILVTDRAFAGADTLATSYALA
                     AAIRKIGQEQAVDLVFTGKQTIDGDTAQVGPGVAKRLGVNLLTYVSRIAEVDLDGRRI
                     QVERRAEGGVQLLESTLPSLITMLEGTNEMRFASMDDMLRAARFPVRKWNKEDAGIED
                     LAKIGLKGSPTIVSKVFGPTPRSTKADMQSVADTSAQDVTLNLLQKIFTAHPMLEEEL
                     VERLSA"
     gene            complement(8698..9036)
                     /gene="nifW"
                     /locus_tag="PKV56_12930"
     CDS             complement(8698..9036)
                     /gene="nifW"
                     /locus_tag="PKV56_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase stabilizing/protective protein NifW"
                     /protein_id="HOX68761.1"
                     /translation="MDGLIQKLKALSSANEFLEFFGVEYDEAVVHVNRLHILKRFYQY
                     LHRAQDLDGLNEIDMFRRYREFLVQAYTDFTTSNAATEKVFKVFQDTDGKQHVTVDAL
                     RSSLGARRAA"
     gene            complement(9067..10221)
                     /gene="nifV"
                     /locus_tag="PKV56_12935"
     CDS             complement(9067..10221)
                     /gene="nifV"
                     /locus_tag="PKV56_12935"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOX68762.1"
                     /translation="MHSPSPSWLLTINDTTLRDGEQTAGVVFTGEEKLAIAHALDDAG
                     VPEIEVGAAAMGDAEIDLIRQITQAGLRARTMVWARMSELDLRAAAHCAADLVHLAVP
                     VSDQQIERKLARDRGWVLGMVHRCVSAARDAGLTPSVGLEDASRADRGFLAQVVQTAQ
                     AAGAVRVRYADTLGILEPFGTFDRISELRRCTDLDIEIHAHDDLGLATANTLAAFRAG
                     ATHASTTVNGLGERAGNAALEEIVMGLRHLHGLECGVDSRALPALSERVAAAAGRPVP
                     AGKCIVGAAVFSHESGIHVDGLLKDRRNYESIDPAELGRSHRIVLGKHSGSAGVRAAF
                     GRLDLQLAEESVPALLERIRELAMRTKRAPDDADLKRLYLDVQSQSRLAA"
     gene            complement(10402..10998)
                     /locus_tag="PKV56_12940"
     CDS             complement(10402..10998)
                     /locus_tag="PKV56_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOX68763.1"
                     /translation="MLSRTVLHRLIGCLAFSLSLAAAAAPASAPDAAPDAPDWVISED
                     GAYAVNLRAQLAWPRCVEGMRWNGKTCTGRPLLLDHAEAIALAGLRSEADGVQWRVPT
                     VTELQRLVLKSARPPGLNPVLFPAAPRDWHWSITANVNTTNVNPYNYGNVMRGRTGEN
                     AIHMAYLHGWAVNLATGKARGDVAKRSELPVRLVRSLD"
     gene            complement(11155..11535)
                     /gene="fdx"
                     /locus_tag="PKV56_12945"
     CDS             complement(11155..11535)
                     /gene="fdx"
                     /locus_tag="PKV56_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOX68764.1"
                     /translation="MDITVAKDVPADGCTTAEITVLPHPELCPQGLVFPARRGRKLVD
                     ELLANGIAIEHACEKVGACSTCHVHVRSGAESLKPPDDEEEDQLDAAWGLDAQSRLSC
                     CVKVRDHALVIELPMYTKNHAREK"
     gene            complement(11542..13452)
                     /gene="hscA"
                     /locus_tag="PKV56_12950"
     CDS             complement(11542..13452)
                     /gene="hscA"
                     /locus_tag="PKV56_12950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOX68765.1"
                     /translation="MALLQIAEPGRAAAPHQHRLAVGIDLGTTNSLVATLRSGLPTVL
                     ADEDGRMLLPSVVHYADGGEIIVGDAARALAIRDPQNTIASAKRLIGRAAADIAPGTV
                     PYTLSELGPGAIGVATRAGVKSAVEVGTEVLRALAQRAEAALGGPLVGAVITVPAYFD
                     DAQRQATKDAAQRAGLTVLRLLNEPTAAAVAYGLDTANEGVYAVYDLGGGTFDVSILR
                     LARGVFEVLSTNGDAMLGGDDFDACVVRWFCAQQPDFAWSERDAAALHAAARSSKESL
                     SSESEVMMRCERSDGVSQMARLTREVFEAETEPLIQRTIAPLKRALRDAGLKPSDIEG
                     IVMVGGATRMPRVRRAVADFFGREPHTDIDPDQAVALGAAIQADQLAGNRAVGDAGLL
                     LLDVCPLSLGLETMGGLVEKIIPRNTTVPTARAQEFTTFKDGQTAMAVHVVQGERELV
                     SECRSLARFTLRGIPPMVAGAARIRVTFQIDADGLLSVSATEQTSGVQTQVEVKPSYG
                     LAHDTVAEMLRSAIDCAESDAAARMLREAEVEARSLLDAVDSALSADESLLMPREQFR
                     ILCALQDVADLLEQCATSEGVNPIEQKDLREGLRQATQALNEATTDFAARRMDARVRE
                     AFSGRSIEQIGG"
     gene            complement(13471..>13548)
                     /gene="iscA"
                     /locus_tag="PKV56_12955"
     CDS             complement(13471..>13548)
                     /gene="iscA"
                     /locus_tag="PKV56_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234802.1"
                     /note="forms iron-sulfur clusters of ferredoxin [2FE-2S];
                     binds iron in the presence of the thioredoxin reductase
                     system; forms homodimers and tetramers; similar to SufA
                     protein; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOX68766.1"
                     /translation="GLNEGFKFNNPNATASCGCGESFAV"
BASE COUNT         2211 a         4421 c         4498 g         2418 t
ORIGIN      
        1 ccaagcggct tgccggtgga gatccgctcg agcgaagggt taggccgcac tcgccgcgac
       61 gtatggcgta accgagacga cagacgctct gccggcgaca aacgaacctg acaagccaaa
      121 gcagtgctcg agttcaaccc tgagttgact cgaacctgct tcgggagcga gaagttcgag
      181 gtcgaggcag gacaaggcgt tctggtggtt cagtccatcg agttcgacgg aggagacatg
      241 ttcgaacagg aagcgaacca ctgagtcgaa ctcgcagcgg tagaagcctt gttctgtgac
      301 ttcgatcgtc atgatccagc cgcgaaggtc gacttcgatg gaagggtagc ggcgaccatc
      361 ggcgtcgcgg cgcgtactgt cgaggaggag gcgatgtatc tcgccatcgt ggaagctggg
      421 ccattggccg aatctggcca agacctcttc atgtccctcg atgaacgcat gcggttccat
      481 gtgcggccta acgtttgagg taagcggctt gccggaggca agtccgcttg accgaatggt
      541 taggtcgcat tcttgatgtg cgccgtgtat tgctcaagct cagcgacgaa gggagccatt
      601 gctgcgcgtg gaagtacgag cgcctgcatt ccatcgatga agagatagta gttagcctcg
      661 tcttccgctc ggaagagcac gccggaccaa ggcacctccg ttgtgccagt cgatgaattt
      721 acatgcaccg aggcggctga cagggaaaca gtttgtggag aaaggaacgc tccgtttggc
      781 gcgagcatga acttgcgaag tgaaacctga gcgacgtacg gcaaagcaac gatggcaagg
      841 agggcaagaa ccagcaggac agcgacggtg ccgagtggct gcgagatctc cgggtactcg
      901 cgcatgaccc gagcgtaggt ggcgccggca aggccgatgc atagccaaac agttacgcga
      961 agaccgaatt gcagcgagaa caacccggcc ttggagcgaa agcgcttggc gaccactttc
     1021 tgcaggcgcg cgaagtcagc gacggtgagt gtgaaagtgg cggacatcgg aatgggtttg
     1081 cgatctaacg ttggagctga ggggccgaaa ccggctggcc gcgcgaagcg atgatgaact
     1141 gctgagcgta gtgcggccag ccggttgagg tccactcgag cgaccagtta ggccgaactc
     1201 gttgtgcacg aaatggtcct gtgcctacct tagccatagc accatcatca cggcggcaag
     1261 agacatgaaa cccaacacct cagccagaac gaatgaactc gctacgtcac tgtactgctg
     1321 gcgcctagcg aactgaatct tcacgaggta cgcaaagctc ccgaagtaga gcgctgcggc
     1381 tagtacggct gcgataacac ctggaaagct ccactcctcc gaggcgacga ccagcaacca
     1441 gagtgcagtc ggcgcagcga cagacggcca tgccccactc aaacgctcgc gaaactgcat
     1501 gtgccgccta acgtgttctc gggagacagt tcgcgccgct taggcgggac ctctgctccc
     1561 tagatcgcca gaccagggtg gatgcaagtg gttgattcat cgccaattct ccgccattta
     1621 ctggataaaa gtacagtatt aaatttccgg caaaagcggt gcgttgttac aggctctcat
     1681 tggacactga cgccgccgca ggacggtcat gatgccagtc agcccgcttg ctttgccacc
     1741 tcgcaagaat ctgcagcctg agcttgcggc ggcccatctg cccccactgc gatcggtgcg
     1801 cgtcctggat caattgcgtg agcggcagag gttcatgcac tacagcctgc gcaccgaaga
     1861 ggtttacgtt tactagacca aggccttcat ccgctttcac ggcacacagc agctggccgc
     1921 gctggccgga gtcagcgcgg ccagctgccg ttgcgcggca aggaactggc agctgtgcag
     1981 accgcgccga tgggtcagga gcgatcgatc cgcgcagtca ccaccggtgt cacgtagtca
     2041 tcgtggtgcc tgccgcaccc gacgcagcat cagaacttgt gcacctcgat gctgtacttg
     2101 cgcagcgcgt agcccagttg ccggggcgtc accttgagca ggcgcgcggc cttggcctgc
     2161 acccaggcac actgctccat cgcccagatc agcctttcgc gctcgccttc gggcggttcg
     2221 tcgccgaact gtcgcggcgt gccgttcagg tgttcggtcg ggccgatgcg cagcacgtcg
     2281 tccgcggcat ccatctccgc ctcgctccag ttctcgtccg attcggccga gtcgcgcacc
     2341 ggcctgggca gatccttgac cgacgaccga acgaccggaa tgtgcagcgg tgtcgacatc
     2401 tgaaccggtg acaccgcatc ggccttgtcc aggtgatgca gcgtctgggt gagacagcgg
     2461 ttctggcggc acgggaacgc gatcccgcgg atcacgtcgc cctgcaccat ggtggcggtg
     2521 cgctcgatgc agttctcgag ttcgcgcacg ttgcccggcc agtagcagtg cgtgagcacc
     2581 tccatcgcat cgccggcgat cttgagcttg cgacggttct ccttgttgta gcgatcgacg
     2641 aagtgcgcca ccagcggcgg gatgtcggca cgccgctcgc gcagcggcgg caggaacacc
     2701 gacaccacgt tgatgcggta gtagaggtcg gcgcggtact cgccgcgctt gaccatcttt
     2761 tccaggtcgc ggttggtggc acagatcaac cgcacgtcca ccttgatcga ccggttgccg
     2821 cccacgcgct cgaattcgcg ctcctgcagc accctcagca gcttggcctg gaacgacggc
     2881 gagacatcgc cgatctcgtc gaggaacagc gtgccggtgt gtgcctgctc gaagcggccc
     2941 ttgcgatcac cgaccgcgcc ggtgaacgcg cctcgctcgt gcccgaaaag ctcgctctcg
     3001 agcagcgact cggtcagcgc cgcacagttg accttgatga aaggcgcatc cttgcgcggc
     3061 gacaggtagt ggatggcgcg cgcgatggct tccttgccgg tgcccgactc gccgcgcagc
     3121 aacaccgtcg cccgcgacgg cgccgcctga tgcacctcgg caaagacctg ctgcatctcg
     3181 gcggacacgc cgaccacgtt atcgagcgcg tagcggcccc gcacctcggg ggccagtgcc
     3241 ttctgcagcc gggtggtctc gagctgcaac cgctggtgtt cggccgcgac cgcgcgcgac
     3301 aggacgatgg tctgcgccag cagggtggca accatcttca ggatgcggtg gtcgtcggac
     3361 aacctcgcgt tgccttcgac ggcacgctgg gccgccagca cccccagaac ttcatggtcg
     3421 gtcttgatcg gcacgaccat gaacgacatc atctggccgt cctgcgcgct gaaggcgccg
     3481 gtgcggtcga tgaactcctc ggcttgggtg acatcgggca ccgtcaccga cacgccgtgc
     3541 tggtacacgc ggccgatcat gccctcgccg acctgccaga caccccgccc ggcctgttcg
     3601 cgatcgaggc cgacgtagct gtgcacccgc aatcgcgagt cgtcctcgtc gggcagcacg
     3661 atcatcacgc gcggcagccc gatctgggca tcgaggatgt tcaaggcagc gcgaaaactg
     3721 cgctgtagat caagggacgc gccaagcact cggcagatct cgtagaccgc gatcagctcg
     3781 atgtgcgaac gctcctgttg actgggtcga atcatgggcg ttctctctca gtttgggcac
     3841 acggacggtg catggtgtgc gctcgtcggt gcatgtgacc ggtcggccct ggaatcacgg
     3901 gtagtgatgc agggagatac gccattcaat gcaaaaactt ggcccggttt gctcccgcca
     3961 acgcagcggc agcgttgtca cgttggcttg acgccgatcg cgtcaagcca gcctgcgtga
     4021 acgcagcgtt cgcgccccgc cgggttcctg gtggggcgct cgtctcctga atccgtcacc
     4081 agacggccgg gtcggcgcgc agcctttcga cgacttcacc gccttcgagg tgcttgtcga
     4141 agatctccgg cacgtcggcc gcggcaacac cgcggtaaag cacgttttcc gggtagacca
     4201 gcacgttggg gccaccgtcg cagggaccca ggcagccgga ataggtgacg gcgatcttgt
     4261 cgtaggcctg acgcttctgc tgctcggccc agaaggcctg cagcagcggc ccggcgccgg
     4321 cagatgcgca cgagccgcgc ggatggttgg gcggacgctg ctggttgcag acaaagacgt
     4381 gacgctgtgg tttgggcatg tcggggctcg atgaatggac aaggcagcgg acgtttcacg
     4441 gtcccgcgtg ttgccggtgg agttgcagcg gattcaggtc gttatcgaac ggcttgctct
     4501 ggcaggcacg cggtgctatc tcgctggtgc ctcggccagc gcaaacaccc agttggcctt
     4561 ggacacgtgg atgggcttga gcacacgccg cggagagatt ccgcgcttga gcgacgccgg
     4621 cttcgccacc aggcccttgg cgatgcggtc gtcgaccagc gacttcaacg agatcgaatc
     4681 gaagtactcg gccagcttcg cgttggcgcc gtcccacagg tcctgcgtca ccggaaggca
     4741 gcttgactct tctgccggct cgctgtcgcg gccgcgggca ccggcgctgc ccgggtcgtc
     4801 gaccgcggag atgatgtcgg ccagcgtgat cgaatccgag tcgcgtgcga gcacgtaacc
     4861 cccgctcggc ccgcgcgtgc tctggaccag ttcatggcga cgcaggcgcc cgaacaactg
     4921 ctccaggtag gacagcgata tctgctgacg ctcgccgaca acggccagcg ccaccggcgc
     4981 cgattccgaa ctgatcgcca gttcgaacat ggccgcgacg gcgacccgtc ccttcttggt
     5041 cagcactccg ttcatcaggg tgttcctccg ttgcggtgcg atgacttcca gccggcgcgg
     5101 acagccgcgc cgctcagccg aacttgaaaa ggatgccgtg gccgccacgc gggtactccc
     5161 aggccacgtt cgtgtgctcg gtgcagatga cacgacagct gccgcactca aggcagccat
     5221 cggtgatcag cgtcaccgcg ccgttgcctt cggccgtgta gcaggccgcc gggcagacgt
     5281 aggtgcattg ctgttcggca cagtcgttcg cgcagatcgc cgcatccttg atctggatgt
     5341 gcggacgacc gtggtcgacc ttgtagcggt tctggaacag cttgtcctcg acattcacgg
     5401 cagcgctcat cgcgtggccc tccagagttt gatggcatcg cccaccaggc cggtgaactt
     5461 gcgggccgac ttgaaactgc cggtgatttc gcgttgcttg gtcatcttgt ccacaccgtc
     5521 gacggtgaac agcgtcttgg cggcgcggtt gacgagttcc gggtaggtgg tgaagaactg
     5581 cgggctcttc tcgagcacgt ccggcaggtt gcggtacttc ttcaggtcct tcatgacaaa
     5641 gctctcgtcg agcgcgtcct tgtaggcctt gagcgtcctg gccgtcatcg ggcggcccgc
     5701 ggcacgggcg gcgatcaccg tctccgcggc cagccgcccg gtggtcatcg ccaggttgga
     5761 gccctcgcgg tgcgccgcgt tgacgaagcc gccggagtcg ccaacgatca tccagccgtt
     5821 gccaaagatc ttcggcacgg cgttgaagcc gccttcggga atcaggtgcg ccgcgtactc
     5881 cttcatttcg ccgccctcga tcagcggggc gaccgaggga tggcgcttga gcttctcgag
     5941 cagcgcgtag ggcgtcgtct tcttcgggtt cgccttgaag tcgctgagca tgcagcccac
     6001 gccgatcgtc agcgagtcct tgttggtgta gaggaacccg gtgccgacca tgccctccgt
     6061 gaccgtgccc atcagctcga tcaccacgcc ctcgtcttcc ttgatgttga agcgggactg
     6121 gatcgtctcc tcgggcatga agaggatttc cttgaccgcc agcgcgacgt tcttcgacga
     6181 gatctcgccg tggaagcccg ccttgcgcgc cagcgtcgag ttcacgccgt cggccaggat
     6241 gaccacgtcg gctcgcacct cgccgcccat gcggtcgcag tgcacgccga ccaccgtgtc
     6301 gccgtccagc agcagatgct cgacggtcgt ctcgcagatc agcagcgcgc ccgcctcgcg
     6361 caccttggac gagaaccact tgtcgaactg cgcgcggatg atcgtgtagc ggttgtaggg
     6421 cggcttgttg tagtcgtcgc tgcggtagtg ggtgccgacg aagctgttgt cgtcgagcat
     6481 ccacatgcgc tgctcgatga tgtgccgctc gagcggcgca tcgtcgcgga agtcgggaat
     6541 gatctgctcg agcgcctggg cgtagaggat cgctccctgc acgttcttcg aacccggata
     6601 ctccccccgc tcgatctgca ggaccttgag gccggccttt gccagcgtgt acgtcgccgc
     6661 gttgcccgag ggtccggcac cgacgacgat cgcatcgaat tgctctgcca tgtcatgtgc
     6721 tcctatgcaa ccttgcggat gcgtttgtcg agatgggtcc ggaaggcctc ggtgagcacc
     6781 ggcagcacct gcatcgcgtt gccgacgatg ccgtagtgcg cgaactcgaa gatcggcgcg
     6841 ttcgggtcgg tgttgatcgc gacgatcacg tccgaactct ccatgccgac gcgatgctgg
     6901 atggctccgc tcacgccggc ggcgatgtag agcttcggcc gcacggtctt gcctgtctgt
     6961 ccgacctggc gatcggcctc gatccagccc gcctgcacga ccggtcgcgt tccgccgacc
     7021 tcggcgccca gcacccgcgc caggtcccag atgagcttga agttgtccgg ctgcttcagg
     7081 cccttgccgc cggtcacgat gacatccgcg taggcgagat tcacggtgtt gcgattggcg
     7141 tccggaatga agtcgagcag cttggtgacg acgtctgtct cgatcatgcc gagcggcatc
     7201 tcgacgccat cgccggtgcg gctcgcgtcg cgccgcggca tctgcatcac gcggggacgg
     7261 atcgtcgcca tctgcggccg gtacgccagc gtcatgatcg tgcagagcag cgaaccgccg
     7321 aacgtcggcc gggtcgctgc cagagcccga ccgtcgatgt tgagttcggt gcagtcggcc
     7381 gtcagcccgg tgcccagcgt ggtggccact gagccggcga gatcgcggcc catcgtcgtc
     7441 gcgccgagca gcatgatctc gggctggtgc ttgttgacca ggtcggtcag gcccttggtg
     7501 aatggttcgt tgcggtagcc cttcagcacc ggatcgcgaa cgaagtagca ccggtcggcg
     7561 ccgtaagcgt aggcctccgc ggcgtaggca tcgaggggct cgtcggggcc acccatcagc
     7621 acgccggcca catcgacctg cagcttgtcg gcgagcttgc gcgcctcgcc catcagctcc
     7681 cagctgaccg ggttgacgtg accgcggtcg tgttcgatga acacccagac gcccttgtag
     7741 gccttcagtg cctcggacag ctcggtattg cggcctgcgc gccccgccgc cttgggcgct
     7801 gcgggtttgg gtgcttcgct catgtctgct ccttaagcac tgagacgctc gaccagctct
     7861 tcttcgagca tcgggtgggc ggtgaagatc ttctgcaaca ggttcagcgt gacgtcctgt
     7921 gcactggtgt cggcaaccga ctgcatgtct gccttggtgc tgcggggcgt cggcccgaag
     7981 accttgctga cgatggtcgg ggagcccttc aggccgatct tcgcgaggtc ctcgatgccg
     8041 gcgtcctcct tgttccactt gcgcaccggg aagcgcgcgg cgcgcagcat gtcgtccatc
     8101 gaggcgaagc gcatctcgtt ggtgccttcg agcatcgtga tcagcgacgg cagcgtcgac
     8161 tcgagcaact gcaccccgcc ttcggcacgg cgctcgacct ggatgcggcg tccgtcgaga
     8221 tcgacttcgg cgatgcggct cacgtaggtc agcaggttga ccccgaggcg cttggcgacg
     8281 cccggtccga cctgcgccgt gtcgccgtcg atggtctgct tgccggtgaa gaccaggtcg
     8341 acggcctgct cctgcccgat cttgcgtatc gcggcggcca gcgcgtacga cgtggcgagc
     8401 gtgtcagcac cggcgaatgc gcggtcggtg accaggatag catcggtggc gccgaagctg
     8461 atgcacttgc gcagcgcgtc ctcggcctgc ggtggcccca tgcacagcat cgtgacgcga
     8521 ccgccgaacc ggtccttcag ccgcagcgcc tcttccagcg cgaacaggtc atacgggttc
     8581 acgatggcag gcacgccctg ccgcatgatg gtgttcgtca ccgggtgcac gcggatctgc
     8641 gcggagtccg gcacttgctt gatgcagacg acgatgtgca tgtcgattcc cttcggatca
     8701 ggcggcgcgc cgggcgccga ggctcgaacg cagggcatcg acggtgacgt gctgcttgcc
     8761 gtcggtgtcc tggaacacct tgaagacctt ctccgtcgcg gcattggagg tcgtgaagtc
     8821 ggtgtaggcc tggaccagga actcgcggta gcggcggaac atgtcgatct cgttcagccc
     8881 gtcgagatcc tgcgcacggt gcaggtactg gtagaagcgc ttgaggatgt gcagccggtt
     8941 cacatgcacc accgcctcgt cgtactcgac gccgaagaac tcgaggaact cgttggcgct
     9001 ggaaagcgcc ttcagctttt gaatcagacc gtccatggtt gcctttcgat gcggtgattt
     9061 cagaggtcag gccgccagcc gcgactgcga ctggacatcc aggtagagac gcttgaggtc
     9121 ggcgtcgtcg ggagcacgct tggtccgcat ggcgagttca cggatgcgtt cgagcagcgc
     9181 gggcaccgac tcttccgcca gttgcaggtc gaggcggccg aaggctgcgc gaactccggc
     9241 cgatcccgag tgcttgccca gcacgatgcg gtggctgcgg ccgagttcgg cgggatcgat
     9301 gctttcgtag ttgcgccggt ccttgagcag cccgtcgaca tggatgccgg actcgtgcga
     9361 gaagacggcg gcaccgacga tgcacttgcc cgccggaacc gggcgaccgg ccgctgcggc
     9421 gacgcgctcc gacagggccg gcagcgcccg gctgtcgaca ccgcactcga ggccatgcag
     9481 gtgccgcagt cccatcacga tttcctcgag cgccgcgttg ccggcgcgct cgccgagccc
     9541 gttcaccgtc gtgctcgcat gggtggctcc ggcgcggaag gcggccagcg tgttggcagt
     9601 ggccagaccc aggtcgtcgt gcgcgtggat ctcgatgtcg aggtcggtgc agcggcgcag
     9661 ttcggagatg cgatcgaagg tgccgaacgg ctcgaggatg cccagcgtgt cggcgtagcg
     9721 cacgcgcacc gcaccggccg cctgcgcggt ctggaccacc tgcgccagga agccgcggtc
     9781 ggcacgcgag gcgtcctcga ggccgacgct cggcgtgagg ccggcatcgc gcgccgcgct
     9841 gacgcaacga tgcaccatgc caagcaccca gccacgatcg cgagccagct tgcgctcgat
     9901 ctgctggtcg gagaccggaa cggcgaggtg cacgaggtcg gcagcacagt gcgcagcggc
     9961 tctcaggtcg agctcggaca tgcgcgccca gaccatcgtg cgtgcgcgca ggccggcctg
    10021 cgtgatctgg cggatcaggt cgatctcggc atcgcccatc gccgcggcac cgacctcgat
    10081 ctcgggcacg ccggcatcgt cgagggcgtg cgcgatggcc agcttctcct caccggtgaa
    10141 gaccacgccg gccgtctgct cgccatcgcg cagggtcgtg tcgttgatgg tcagtagcca
    10201 ggaagggctg gggctgtgca tggcgggctc cgaaggtcgg cacacgcttc atcgcaagcg
    10261 ctgtgccatc acctcgagct cgctaagcca ttgaatacgc gagatttttc cgattcggat
    10321 cgatgtgtat cgattgtttg gttgtcgaca gaaccggccg tgcgggcatt catccacggg
    10381 ccggccgaca gccctcgaag ctcagtccag cgagcgcacc aatcgaaccg gcaactcgct
    10441 gcgcttggcc acgtcgccac gggccttccc ggtcgccagg ttcaccgccc agccatgcag
    10501 gtaggccatg tggatcgcgt tctcaccggt gcgaccccgc atgacgttgc cgtagttgta
    10561 ggggttgacg ttggtcgtgt tgacgttggc cgtgatcgac cagtgccagt cgcgcggcgc
    10621 ggcgggaaag agcaccgggt tcaggccggg cgggcgggca gacttgagca cgagtcgctg
    10681 caactcggtg acggtcggca cgcgccactg cacgccgtcg gcctcgctgc gcagacccgc
    10741 cagcgcgatg gcttcggcgt gatcgagcag caggggccgg ccggtgcagg tcttgccgtt
    10801 ccaccgcatg ccttcgacgc agcgcggcca cgcgagctgg gcacgcaggt tgacggcgta
    10861 ggcgccgtct tccgatatca cccagtccgg cgcgtcgggc gccgcgtcgg gtgccgaagc
    10921 gggggcagcc gcggccgcca gggacaggct gaaagccagg cagccgatca gtcggtgaag
    10981 gacggttctc gacagcatgg tgagcaggcg gttcagtgac gcgagcggcc cggcggcgcc
    11041 acggcgccgc cgcacgcgcg ggcggaagcg gtacagccta caggttgctc tgcccgggca
    11101 ccatgacatg gatcacggtc ctggcatggc acggcgcgcc cggcgccggt gccgtcactt
    11161 ctcgcgtgcg tggttcttcg tgtacatcgg cagctcgatc acgagcgcgt ggtccctgac
    11221 cttcacgcag caagacagcc gtgactgcgc gtcgaggccc caggcggcgt cgagctggtc
    11281 ttcctcctcg tcgtccggcg gcttcaggga ttcggccccc gaccgaacat gcacatggca
    11341 ggtcgaacag gcgcccacct tctcgcaggc atgctcgatc gcgatgccgt tcgcgagcag
    11401 ttcatcgacc agcttgcggc cgcgccgagc cggaaagacc agtccctgcg gacagagctc
    11461 gggatgcggc agcacggtga tttcggcggt ggtgcatccg tccgccggca cgtccttcgc
    11521 cacggtgatg tccatgctgt cctagccgcc gatctgctcg atgctgcgcc cggaaaacgc
    11581 ttcgcgaacg cgtgcatcca tgcgccgggc cgcgaagtcg gtcgtggcct cgttcagagc
    11641 ctgcgtcgcc tggcgcaagc cctctcgcag atccttctgc tcgatggggt tgaccccctc
    11701 gctggtggca cattgctcga gcaggtccgc gacgtcctgc aacgcgcaca ggatgcggaa
    11761 ctgctcgcgc ggcatcagca gcgactcgtc ggcactcagc gcgctgtcga ccgcatcgag
    11821 caggctgcgc gcttcgacct cggcctcgcg cagcatgcgc gctgcggcgt cggactcggc
    11881 gcaatcgatc gcgctgcgca gcatctcggc caccgtgtcg tgggccagac cgtagctcgg
    11941 cttgacctcg acctgtgtct gcacgccgct cgtctgctcg gtggcgctca ccgagagcag
    12001 gccatcggca tcgatctgga acgtgacccg gatgcgcgct gcgccagcga ccatcggcgg
    12061 aatgcctcgc agcgtgaagc gcgccagcga gcggcactcg ctgaccagct cccgttcgcc
    12121 ctggacgacg tggaccgcca ttgcggtctg gccgtccttg aaggtcgtga attcctgtgc
    12181 ccgggcagtc ggaacggtcg tgttgcgcgg aatgatcttc tcgaccagcc cgcccatcgt
    12241 ctccagtccg agcgacagcg ggcagacgtc gagcagcagc aggccggcgt cgccgacagc
    12301 ccggttgccc gccagctgat ctgcctggat ggcggcaccg agtgcaaccg cctgatcggg
    12361 atcgatgtcg gtgtgcggct cgcggccgaa gaagtcggcc accgcgcggc gcacccgcgg
    12421 catgcgcgtg gcgcccccga ccatcacgat gccttcgatg tcggagggct tcagtcccgc
    12481 gtcgcgcagc gcacgcttga gcggtgcgat ggtgcgctgg atcagcggct cggtctcggc
    12541 ctcgaagacc tcgcgtgtca gccgcgccat ctgcgagacg ccgtcgctgc gctcgcagcg
    12601 catcatcact tcggactcgc tgctcaggga ttccttgctg ctgcgggcgg ctgcgtgcag
    12661 cgccgcggca tcgcgctcgc tccaggcgaa gtccggttgc tgggcacaga accagcgcac
    12721 gacgcaggcg tcgaaatcgt cgccaccgag catcgcgtca ccgttggtgg acagcacctc
    12781 gaagacgccg cgagccaggc gcaggatcga tacgtcgaac gtgccaccac ccaggtcgta
    12841 gacggcgtag acgccttcgt tggcggtgtc gagcccgtag gcgaccgccg cagccgtggg
    12901 ctcgttgagc agacgcagca ccgtcaggcc cgctcgctgt gcggcgtcct tggtggcctg
    12961 gcgctgcgca tcgtcgaagt aggccggcac cgtgatcact gcgccgacca gcggaccgcc
    13021 gagcgcggcc tcggcgcgct gcgccagggc gcgcagaacc tcggtgccga cttcgaccgc
    13081 gctcttcacg cccgctcgcg tcgccacgcc gatcgcgccc gggccgagtt cgctcagcgt
    13141 gtagggaacg gtgcccggcg cgatgtcggc cgcggcgcgt ccgatcaggc gcttggccga
    13201 tgcgatggtg ttctgcggat cccggatcgc cagtgcgcgg gccgcatcgc cgacgatgat
    13261 ttcgccaccg tcggcatagt ggacgaccga gggcagcagc atgcggccgt cttcatcggc
    13321 cagcacggtg ggcaatccgc tgcgcagcgt ggccaccagc gaattggtcg tgcccaggtc
    13381 gatgcccacg gccagccggt gctggtgggg agcggccgcg cgacccggct cggcgatctg
    13441 cagcaaggcc atcgcagcgt tccgtggggt tcagacggcg aacgactcgc cgcagccgca
    13501 gctcgcggtg gcgttcgggt tgttgaactt gaagccctcg ttcaggcc
//
LOCUS       DAOORV010000101        13339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_5706_length_13339_cov_9.066546, whole genome shotgun sequence.
ACCESSION   DAOORV010000101 DAOORV010000000
VERSION     DAOORV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_13339_cov_9.066546"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..464)
                     /locus_tag="PKV56_12960"
     CDS             complement(276..464)
                     /locus_tag="PKV56_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68767.1"
                     /translation="MASRVLNSTRLGIAISGTRSSYIVTQQLVDATMAAPAGRKDHPS
                     AADYDPNLPPAPRAAAQH"
     gene            662..2416
                     /locus_tag="PKV56_12965"
     CDS             662..2416
                     /locus_tag="PKV56_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68768.1"
                     /translation="MSAGFIGLAAGHIDAAIEDALRRIVLALDIDRSSLISVDAVTGR
                     IETTHSWALEGIDRTPPTDIVQSNPWALSCARAGRPVVFARLDELPAQAAVDKSTFAR
                     MGLKSHVGMPMWVGGRLRGSLTFGSLRREREWPDELLGRMRLLADVFASVLARQHAEQ
                     QRERVLDFERLASRTLSALLIAMPQDEARIIEAGLHDIAAFLGADRATLWEIGDDQRF
                     RRAHHWFADGVPGIEEWSAQALPWIHARLNTDALVRFARLGELPADADADLQAMRDLG
                     VRSLLAVPVAVAGQLLGVFSVAAVRVECDWPDELVPSVRLLAEVFATLHVRRHAERRE
                     RAAESEAALWREQFAHVVRVHTVGEMSAALAHEITQPLGAIENYAHAARRRLSGPAPD
                     LPKVVDLLERIVAQSARAGDVVTRLRGMVKRHELQLGEIDLERLATACIDLLRGECEQ
                     HDLRIELRRPGRLPRLVADEVHVQQVLLNLLRNAIEAMEAAAPAAPRRIEVNIACVAP
                     EWVSVQVADHGPGIAEGELEQVFEAFHSTKPLGLGVGLAICRRLIEAQGGTLRAAQNP
                     AGGALFEFTLPVAPAESA"
     gene            2413..3048
                     /locus_tag="PKV56_12970"
     CDS             2413..3048
                     /locus_tag="PKV56_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX68769.1"
                     /translation="MTEPTIFIVDDDEAYRDSLVELVASVGLHCECFASALDFLAAFD
                     PARAGCLVLDVRMARMSGLALQARLKTMGATLPIVFISGHGDLEMAVKAIKDGAVDFV
                     QKPYREQQLLDAINEALQRDAQQRRTPLPADGAAPSVALALLSAREREVMQLALQGLP
                     SKLIAKQLCLSHRTVEHHRSRLLEKLGVASMAELMRLNYQQALDGSAAPPR"
     gene            3119..3502
                     /locus_tag="PKV56_12975"
     CDS             3119..3502
                     /locus_tag="PKV56_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX68770.1"
                     /translation="MFNTVYVLERDPAECRWIESALAGQVRKLIFLEDGATLLDWLAT
                     QGGDCLLCGAEPDASVALELVRRLRRHGQALPVVVIGPHSAFRTAVDVARLNGTDFLE
                     RPVSAQRLRAALRKVTAVTEEGAKR"
     gene            3517..4992
                     /locus_tag="PKV56_12980"
     CDS             3517..4992
                     /locus_tag="PKV56_12980"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HOX68771.1"
                     /translation="MNDSTRQAQQALEAVERVQRLYAAQRAACLAQPYPSADERLERL
                     RALKRQTQRYQDLLAEAMSADFGFRPAAESKMLDLLGSVLEANHAVSHLKRWMKPSKR
                     STELLFLGNSVKVLYQPKGVVGVIVPWNFPIYLAMGPLVTALAAGNRVMIKMSEVTPA
                     TNAVFKTLLGEVFGEDLVAVVGEELVDPNVFTSLPFDHIVFTGSPAVGKIVMRTAAHN
                     LTPVTLELGGKSPAMVTRNYPVADAALRITHGKSVNCGQICIAPDYALVPRESVDAFV
                     ASVKSSFEQFYGGHTEGNPNFTSVVSDRQLQRIEAVLADARAKGATVTACAPQPSAGN
                     RQLPLHIVTGATPDMDVLKEELFGPVLPVVPYDSTDDAIRFINERPRPLALYCFSHDN
                     AERDDLLRRTHSGGVSVNDWGWHAINHDAPFGGIGNSGIGNYHGEEGFRELSHARTVF
                     QRRRWFPTQLFHPPYGSFVQRLSMKFLLGEADPSLGARSKH"
     gene            5017..6480
                     /locus_tag="PKV56_12985"
     CDS             5017..6480
                     /locus_tag="PKV56_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOX68772.1"
                     /translation="MPALSAIDLAMFMLETRERPFNIGPLVLLHPPAGFKGSFADKLH
                     ARLLKRPPGPPFNYRLVLSLTHAPSVEPMANPDIRGHVHRLTLQDASMAQLLEKVCEL
                     HEAQLDRSGLLWHFYVIDGLADGSVALYGKVHHGIIDGRTFVKAVTQWMSEDPKDKEV
                     RAMWDGVARSAKGAGQRAGMVQRLVAAGSQAVGLVRSAAGVYGLLAEQGLRSAGLGKG
                     LPLPFLNVPGAFDGKLSARRSYAYCTLPLAELKAVGKAHGATVNDMLLTVLDIAMQRY
                     LAKQKKVAREPLVADMPVALAEGAKGGNQIAVLQFPLGQAAASVSDRLSAIRAETGHL
                     KDALGKRDSDTVMLYTTLVHALPLLVERVAENAAPRLSNLLISNPFGFAGEAYLMGAK
                     VELALPISVVAAGHKLNVTAVTLGPRLQIGFLAMPAAVPHIDQLARLTEQAFDEVKAA
                     LLPAAKAVLPKVATRKAPAKARGPVAAKRKAGAARAH"
     gene            6515..8491
                     /locus_tag="PKV56_12990"
     CDS             6515..8491
                     /locus_tag="PKV56_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOX68773.1"
                     /translation="MRQAQRIATSLLGAALLAAGCASAPGSADLTPKPATPATVNANQ
                     AMRPVVAAFDKRDFDDATRGKLAELNDPLVKAADGRIVWDTRKFDFLKGETPVTANPS
                     LWRQQQLNHAQGLFKVADGIYQIRGYDIAGMTLVEGQTGWIVIDTMLTAPMARAGFKL
                     AMDTLKSAKPVVAVIYTHSHADHFGGVRGVVDEADVRSGKVKIIAPDAFMEHAIAENV
                     LAGNAMTRRAQYMYGTALPTSGQGTLGTGLGLALSNDSPTLIPPTQYIKKTGEEMTID
                     GVRIVFQMAKGSEAPSEFMFYFPDKKALCLSEVATKLMHNVYTIRGAQVRDALQWSKY
                     INETMDLFPDAEVAFASHHWPTWGKDNIRAFLTNQRDTYRFLHDRALNLANQGLVMDE
                     IGSEPFFPKGLAVDAASRGYYGTLSHNLRAVYNFYLGYYDANPATLHRLPPAESAKRY
                     VAAMGGEAAALAAGRKAFADGDYRWVAEMVNHVVFANPGNAEARALQADALEQLGYQA
                     ESATWRNAYLMGAQELRAGPKPFGGSSANPDTVRGMSNELLFDFIALRLDHTKTDGMK
                     AAVQMVFTDVNETWALELSNSVLNNTKGRVLKNPDVKITLTRPAFLAMLLQGKKLPEL
                     VQAGMVKIDGNPQGFGAVVANIVEFPPLFNIVTP"
     gene            8502..10124
                     /locus_tag="PKV56_12995"
     CDS             8502..10124
                     /locus_tag="PKV56_12995"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOX68774.1"
                     /translation="MLQPGLMMDQPLLLSSVIEHAAAQYGDVEVVSRETHGPLFRYTY
                     ADCAKRARKLAHALGQLGLSGGSAVGSIAWNNHRHLEAYYAVSGSGMVMHTCNPRLHP
                     QQLIYVINHAEDQVMLFDATFAPLIKGIASHCPKVKAWVCLADKANTPAIEGVANVLS
                     YEELIAPHSEHFEWPQFDERSGAALCYTSGTTGNPKGALYSHRAIVLNALVGSTPGVL
                     ALSARETILPVVPMFHINAWCIPYGAPMVGARLVLPGPKLDGASLYELMESERVTVSA
                     GVPTIWLALLQHVEANKLRFTTMRRTAVGGSAMPLSLIAKFADEYGVEVRHGWGMTET
                     TAVATMSALMSKHDPLSAADKHAVVAKQGKAVFGVQIKVVDETGATLPRDGSSQGELM
                     VRGQWIVSGYFKGESSPLQDGWFPTGDVATIDADGVMQIRDRAKDVIKTGGEWISSID
                     LENAAMGHPGVAMAAVIGVKHPKWDERPLLFIVRKPGQALEKDEILAFLAERVAKWWV
                     PDDVVFVDSLPVGGTGKVQKADLRKQYGGVFS"
     gene            10137..10703
                     /locus_tag="PKV56_13000"
     CDS             10137..10703
                     /locus_tag="PKV56_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68775.1"
                     /translation="MPIEPAEDRGLPRRDFLKVGLGFSLALTVGTAVVGCSDAAKTLS
                     TGYVFLRPGDVELFSALTPAVVTELGAFDAAKRSKLVERTLQNIDGTCAALDLGSRQE
                     LRKLLDLLAIGPLRYSLTGVGAWNEAGIDKMQVFLARWRGSRFATLNAGGNVLVKLTS
                     SSFYVLPESWPSTGYPGPLEYVFNAINS"
     gene            10713..12305
                     /locus_tag="PKV56_13005"
     CDS             10713..12305
                     /locus_tag="PKV56_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOX68776.1"
                     /translation="MALPDIYTEGIASGWKVVDAAKLTAPQTMEADVAIIGSGAGGGV
                     AAEILALAGLKVLLLEEGALRTSDSFKDMDEGRALRELYQEGAARATSDGAIAVLQGR
                     SVGGSTTVNWTSSFRTPEPTLKFWAAEREVVGHGVDDMKPWFEKMEARLDVMLWEAQN
                     ANNSVLKRGCDKLGWEAHSIPRNVRGCWNSGYCGFGCPVNAKQSMLVSTIPEALRHGA
                     TLVHRLRVRQLQHAGDKITGAVGEALREDGRTPSGTLVTIKARHFIAAGGAINTPALL
                     LRSQLPDPHKRLGQHTLIHPVVLSLAQMPERIDPFYGAPQSTASDHFQWKDGATGPMG
                     YKLEVPPIFPGIGAGVLNVYGDDLRKEMAALPHTNAVLALLRDGFVEQSPGGSIRLAD
                     DGSPMLDYELSDYVWEGVKRAYLSMAELQFAAGAQRVRQAHIDASYQTSWTDAKKAIA
                     ELPLKKFRATLFTAHLMGGCAMSQNPKRGVVDSGGRHHQLANLSVMDGSVFPTSIGAN
                     PQLSIYGLVAQNATALAKQLKT"
     gene            12307..12954
                     /locus_tag="PKV56_13010"
     CDS             12307..12954
                     /locus_tag="PKV56_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68777.1"
                     /translation="MSEAAPPLAAVPRAAAVAGVAFALLFGAALLLVRLSIPGSIVER
                     GGWLADPAGNLRLATDLLAWGGIAFLWFIGVVRTHIGAREDRFLASVYFGSGVLFLGM
                     SFIAGATAQALLAAHAQYGERFFDSGTFAFGGRLAYELTNTYALRMAGVFMFSLATLC
                     HRTGAMPRVFVWATWTLAALLVLGLTQTLWAALLFPAWVLGFSLYLLLRPGPAQA"
BASE COUNT         2200 a         4436 c         4608 g         2095 t
ORIGIN      
        1 atgggacggt cagctgacct tcagcggcga caggtgacgg gcggccaacg acccagagcg
       61 ggcacacagg ttgtgtgact cggtgccaca aaggcgtcct tcgatagctc gcatcgaatt
      121 caagagcgcc gggccagact cctggcgccg gtcccggggc ggcctgacgc ttccgtcgcg
      181 gccctgatga caatctcggt actcgacggt gtcgcgactg gctccggtca tttcggccgc
      241 gcaaggccat cgcagagagc gatcatgtgt agcgctcaat gctgggcggc ggcccggggt
      301 gccggcggta agttggggtc gtaatcggcg gcgctgggat ggtccttgcg gcccgcaggt
      361 gccgccatgg tggcgtcgac caactgctgc gtgacgatgt acgagctgcg ggttccggag
      421 atggcaatgc ccagccgggt tgaattcagt acgcgagatg ccactgcaga ctagaagatg
      481 ccggcatcga ggctcgctgc atccccgtgt gccagctacg atggcgtgcg agccttcgcg
      541 gctgcaggaa cgcgacaccg ccgtgccgaa cccagacacc accctaccag cgcccaaggc
      601 gaacccgagt ccacattcgt cgaatgcgga actgcaggtc ttcgaacgac tgctcgcaga
      661 attgtcggcc ggcttcatcg gcctggctgc cggacacatc gacgcggcga tcgaagacgc
      721 gctgcgccgc atcgtgctgg cgctggacat cgaccgctcc tcgctgatct cggtggatgc
      781 ggtcaccgga cgcatcgaga ccacgcattc ctgggctttg gaaggcatcg atcgaacgcc
      841 gccgacggac atcgtgcaga gcaatccctg ggccttgagc tgcgcgcgcg ccggccgccc
      901 cgtggtcttt gcccgcctgg atgagttgcc ggcgcaagcg gcggtagaca agtccacctt
      961 tgctcgcatg ggactgaagt ctcatgtcgg catgccgatg tgggtgggcg ggcgcctgcg
     1021 cggcagcctg accttcgggt cgttgcgccg cgaacgcgaa tggccggacg agttgctggg
     1081 ccgcatgcgg ctgctcgccg atgttttcgc cagcgtactg gcgcgccagc acgccgaaca
     1141 gcagcgcgag cgcgtgctcg acttcgagcg tctggcctcg cgcacgctga gcgcactgct
     1201 gatcgctatg ccccaggacg aagcgcgcat catcgaagcc ggcctgcacg acattgcggc
     1261 cttcctcggc gcggaccggg cgacgctgtg ggaaatcggc gacgaccagc gcttccgtcg
     1321 cgcgcaccac tggttcgccg acggcgtgcc cggcatcgag gaatggtcgg cgcaggctct
     1381 gccgtggatc catgcccgcc tgaacacaga cgccctggtg cgcttcgcac ggctgggcga
     1441 actgccggca gatgccgacg ccgacctgca ggcgatgcgg gacctgggcg tgcgttcgct
     1501 gctggcggtg ccagtggcgg tcgccggcca gctgctgggg gtgttctcgg tcgccgcggt
     1561 gcgggtcgaa tgcgactggc ccgacgagct cgtgccgagc gtgcgcctgc tggcggaagt
     1621 ctttgccacg ctgcatgtgc ggcgccatgc cgagcgccgc gaacgtgccg ccgaatcgga
     1681 agccgcgctg tggcgcgagc agttcgcgca tgtcgtgcgg gtgcacaccg tcggcgagat
     1741 gtcggcggcg ctagcgcatg agatcaccca gccgctgggg gcgatcgaga actacgcaca
     1801 tgccgcacgg cgccgcctgt ccgggcctgc gcccgatctg ccgaaggtgg tcgacctgct
     1861 ggagcgcatc gtcgcgcagt cggcacgtgc gggcgacgtg gtcacgcggc tgcgcggcat
     1921 ggtcaagcgc cacgagctgc agctcggcga gatcgacctg gagcgcctcg ccacggcctg
     1981 catcgatctg ctgcgcggtg aatgcgagca gcacgacctg cgcatcgagc tgcggcggcc
     2041 gggccggctg ccccgcctgg tggccgacga ggtgcatgtg cagcaggtgc tgctgaacct
     2101 gctgcgcaat gccatcgagg cgatggaggc ggcggctccc gctgcgccgc gccgcatcga
     2161 ggtgaacatc gcctgcgtcg ccccggaatg ggtgtcggtg caggtggccg accatggccc
     2221 cggcattgcc gagggcgagc tggaacaggt gtttgaagcc ttccactcca ccaagccgct
     2281 gggtctgggc gtcggcctgg cgatctgccg gcggctgatc gaggcgcagg gcggcacgct
     2341 gcgcgcggcg cagaatccgg cgggcggcgc gttgttcgag ttcacgctgc ccgtcgcccc
     2401 tgcggagagc gcatgaccga gcccaccatc ttcatcgtcg acgacgacga ggcctatcgc
     2461 gactcactgg tcgagttggt cgcatcggtc ggcctgcact gcgagtgttt tgcgtcggcg
     2521 ctggatttcc tggccgcctt cgacccggcc cgcgctggct gcttggtgct cgacgtgcgc
     2581 atggcccgaa tgagcggcct ggcgctgcag gctcggctga agacgatggg cgccacgctg
     2641 cccatcgtct tcatcagcgg ccatggcgac ctggagatgg cggtcaaggc gatcaaggac
     2701 ggcgcggtcg acttcgtgca gaagccttac cgcgagcagc agctgctcga tgccatcaac
     2761 gaggccttgc agcgcgacgc gcagcagcgc cgcacgccgc tgccggccga tggggcggcg
     2821 cccagcgtgg cgctggcgct gctcagcgca cgcgagcgcg aggtcatgca gctggcgctg
     2881 cagggcctgc cgagcaagct gatcgccaag cagttgtgcc ttagccaccg taccgtcgag
     2941 catcaccgca gccggctgct ggaaaagctg ggtgtggcct cgatggccga gctgatgcgg
     3001 ctgaactacc agcaggcgct ggacggcagc gcagcgccac ctaggtaggc ttggggtggg
     3061 cgcacggatg gtgcgcccgg cagcggcgca gcaccatcgc ggtgctgcga gggaggcgat
     3121 gttcaacacc gtgtatgtgc tcgaacgcga cccggccgaa tgccgctgga tcgagtccgc
     3181 gctggcgggt caggtgcgca agctgatctt cctggaagac ggcgccacgc tgctcgactg
     3241 gctggcgacg caggggggcg attgcctgct ttgcggcgcc gagcccgatg cgtcggtggc
     3301 cctcgaactg gtgcgcaggc tgcgccggca cgggcaggcc ttgccggtgg tggtgatcgg
     3361 gccgcacagc gccttccgca cggcggtgga cgtggcgcgc ctgaacggca ccgacttcct
     3421 ggagcgcccg gtcagcgcgc agcgcctgcg cgccgcgctg cgcaaggtga cggcggtgac
     3481 cgaagaaggc gcaaagcgct gaacgaggag acgaatatga acgacagcac acgccaagcg
     3541 cagcaggcac tggaggcggt ggaacgggtg caacggctct atgccgcgca gcgcgccgcc
     3601 tgtctggcgc agccctaccc gagcgccgac gagcggctcg agcggctgcg cgcgctgaag
     3661 cgccagacgc agcgctacca ggacctgctg gccgaagcaa tgagcgccga cttcggcttc
     3721 cgccccgcgg ccgaatcgaa gatgctggac ctgctgggtt cggtactcga agccaaccac
     3781 gcggtctcgc acctgaaacg ctggatgaaa cccagcaagc gctcgaccga gttgctgttc
     3841 ctcggcaaca gcgtgaaggt cctgtaccag ccaaagggcg tggtcggcgt gatcgtgccg
     3901 tggaactttc cgatctacct ggcgatgggc ccactggtca ccgcgctggc cgcaggcaac
     3961 cgcgtgatga tcaagatgtc ggaggtgacg ccggcgacca acgcggtgtt caagacgctg
     4021 ctcggcgagg tctttggcga agacctggtg gcggtggtcg gcgaggaact ggtcgacccg
     4081 aacgtcttca cctcgctgcc cttcgaccac atcgtcttca ccggctcgcc ggcggtcggc
     4141 aagatcgtca tgcgcaccgc ggcgcacaac ctgaccccgg tgacactgga actcggcggc
     4201 aagtcgccgg cgatggtcac gcgcaactat ccggtggccg atgcggcgct gcgcatcacg
     4261 cacggcaagt cggtcaactg cgggcagatc tgcatcgcgc cggactacgc gctggtgccg
     4321 cgcgagagcg tcgatgcgtt cgtagcgtcg gtcaagagca gcttcgagca gttctacggc
     4381 ggccacaccg agggcaaccc caacttcacc agcgtcgtca gcgaccggca gctccagcgc
     4441 atagaggcgg tactggccga cgcgcgcgcc aagggcgcca ccgtcaccgc ctgcgcgccg
     4501 caaccgtcgg ccggcaaccg gcaactgccg ctgcacatcg tcaccggggc gacgccggac
     4561 atggacgtgc tgaaggagga gctgtttggc ccggtgctgc cggtagtgcc ctatgacagc
     4621 accgacgacg ccattcgctt catcaacgag cgtccgcgcc ccttggcgct gtactgcttc
     4681 agccacgaca atgccgagcg cgacgatctg ctgcggcgca cgcattcggg cggcgtctcg
     4741 gtcaacgact ggggctggca cgcgatcaac cacgacgcgc ccttcggcgg catcggcaac
     4801 tcgggcatcg gcaactacca cggcgaagag ggctttcgcg agctgtcgca cgcgcgcacg
     4861 gtgttccagc gccgccgctg gttcccgacg cagctgttcc acccgcccta cggcagcttc
     4921 gtgcagcgcc tgtcgatgaa gttcttactc ggcgaagccg acccgtcgct cggcgccagg
     4981 tccaagcact gacatcccta gctgcaggag agcgagatgc ccgcactttc ggccatcgat
     5041 ctggcgatgt tcatgctcga gacgcgcgag cggcccttca acatcggccc gctggtgctg
     5101 ctgcacccgc ccgcgggctt caagggcagt ttcgccgaca agctgcacgc caggctgctg
     5161 aagcgcccgc ccggcccgcc gttcaattac cggctggtgc tgtcgctgac ccacgcgccg
     5221 tcggtcgagc cgatggcgaa cccagacatc cgcggccacg tgcaccggct gacgctgcag
     5281 gatgcgtcga tggcgcagct gctcgagaag gtctgcgagc tgcacgaggc gcaactcgac
     5341 cgctcggggc tgctgtggca tttctacgtg atcgacggcc tcgccgacgg cagcgtggcg
     5401 ctgtatggca aggtgcacca cggcatcatc gacgggcgca cctttgtcaa ggcggtgaca
     5461 cagtggatgt ccgaggaccc gaaggacaaa gaggtgcgcg cgatgtggga cggcgtggca
     5521 cgctcggcca aaggcgcagg ccagcgtgcc ggcatggtgc agcggctggt cgcggccggc
     5581 tcgcaggcgg tgggcctggt gcgttccgcc gccggcgtgt acgggctgct cgccgagcag
     5641 gggctgcgct cggccggcct cggcaagggc ctgccgctgc ctttcctgaa cgtgcccggc
     5701 gccttcgacg gcaagctctc ggcccggcgc agttatgcct actgcacctt gccgctggcc
     5761 gagctgaagg ccgtcggcaa ggcccatggc gcgaccgtca acgacatgct gctgaccgtg
     5821 ctcgacattg ccatgcagcg ctacctggcc aagcagaaga aggtcgccag ggagccactg
     5881 gtggccgaca tgcccgtggc gctggccgag ggcgcgaagg gcggcaacca gatcgcggtg
     5941 ctgcagtttc cgctcggcca ggccgcagcc tcggtgagcg accgcctgag cgcgatccgt
     6001 gccgagactg gccacctgaa ggacgcgctc gggaaacgcg acagtgacac ggtgatgctc
     6061 tacaccacac tggtgcacgc gctgccgctg ctggtggagc gggtggccga gaacgcggcg
     6121 cccaggctgt caaacctgct gatctcgaat cctttcggct ttgcgggcga ggcctacctg
     6181 atgggggcga aggtcgaact ggcgttgccg atttcggtgg ttgcggccgg gcacaagctc
     6241 aacgtcactg cggtcaccct cgggccgcgt ctgcagatcg gttttctggc gatgcccgcg
     6301 gcggtgccgc acatcgacca gctggcgcgc ctgaccgagc aggccttcga tgaggtcaag
     6361 gccgcgctgc tgcctgcggc caaggccgtg ttgcccaagg ttgccacacg caaggcgccg
     6421 gccaaagcgc ggggcccggt tgctgccaag cgcaaggccg gcgcggcgcg cgcccactga
     6481 tccacccaac cccaatccaa gaaggaggac atcgatgaga caagcccaac gtattgccac
     6541 ttcgctgctc ggtgcggcac tgctcgctgc cggctgcgcg tccgcacccg gttcggccga
     6601 cctgacgccc aaaccggcca cgcccgccac cgtcaacgcc aaccaggcca tgcgccctgt
     6661 ggtggcagcc ttcgacaaac gcgacttcga cgacgccacg cgcggcaagc tggccgagct
     6721 gaacgacccg ctggtcaagg cggccgacgg ccgtatcgtc tgggacacgc gcaagttcga
     6781 tttcctgaag ggcgagacgc cggtcactgc caaccccagt ctgtggcgcc agcagcagct
     6841 gaaccatgcg cagggcctgt tcaaggtggc cgacggcatc taccagatcc gcggctacga
     6901 catcgccggc atgacgctgg tcgaaggcca gaccggctgg atcgtgatcg acaccatgct
     6961 caccgcgccg atggctcgcg ccggcttcaa gttggcgatg gacacgttga agagcgccaa
     7021 gccggtggtg gcagttatct atacccacag ccatgccgac catttcggcg gcgtgcgcgg
     7081 cgtggtcgac gaggccgacg tgcgctcggg caaggtaaag atcatcgcgc ccgatgcctt
     7141 catggagcat gcgatcgccg agaacgttct cgctggcaac gcaatgacgc ggcgggcgca
     7201 gtacatgtac ggcacggcct tgccgaccag cggccagggc acgctgggca ccgggctcgg
     7261 gctggccttg agcaacgact cgccgacgct gatcccgccc acgcagtaca tcaagaagac
     7321 cggcgaggag atgacgatcg acggcgtgcg catcgtcttc cagatggcca agggttccga
     7381 ggcaccgtcg gagttcatgt tttacttccc cgacaagaag gcactgtgcc tgtcggaggt
     7441 ggccacgaag ttgatgcaca acgtctacac cattcgcggc gcgcaggtgc gcgacgcgct
     7501 gcagtggagc aagtacatca acgagacaat ggacctgttc cctgacgccg aggtggcctt
     7561 cgccagccac cactggccca cctggggcaa ggacaacatc cgcgcgttcc tgaccaacca
     7621 gcgcgacacc taccgcttcc tgcacgatcg cgcgctgaac ctggccaacc agggactggt
     7681 gatggacgag atcggcagcg agcccttttt ccccaagggc ctggcggtgg acgccgcctc
     7741 gcgcggctac tacggcacgc tcagccacaa cctgcgcgcg gtctacaact tctacctcgg
     7801 ctactacgac gccaacccgg ccacgctgca tcgcctgccg ccggccgaaa gcgcaaagcg
     7861 ctatgtggcg gcgatgggcg gcgaggcggc ggcgctggcg gccgggcgca aggccttcgc
     7921 cgacggcgac taccgctggg tggccgagat ggtcaaccac gtggttttcg ccaaccccgg
     7981 caatgccgag gctcgcgcgc tgcaggccga tgcgctggag cagctgggct accaggccga
     8041 atccgccacc tggcgcaacg cctacctgat gggtgcgcag gagctgcgcg ccgggcccaa
     8101 gcccttcggc ggcagctcgg ccaacccgga cacggtgcgc ggcatgagca acgagctgct
     8161 gttcgacttc atcgcactgc gcctggacca caccaagacc gacggcatga aggcggcggt
     8221 gcagatggtc ttcaccgatg tcaacgagac ctgggcgctg gagctgtcga actcggtgct
     8281 gaacaacacc aagggccgcg tgctgaagaa ccccgacgtg aagatcacgc tgacgcggcc
     8341 ggccttcctg gcaatgctgt tgcagggaaa gaagctgccc gagctggtgc aggccggcat
     8401 ggtgaagatc gacggcaacc cgcagggctt cggcgcggtg gtggccaaca tcgtcgagtt
     8461 cccgccgctg ttcaacatcg tcacaccctg aggagaacac catgcttcaa cccggtctga
     8521 tgatggacca gccgctgctg ctgtccagcg tgatcgaaca cgccgccgcg cagtacggcg
     8581 acgtcgaggt cgtctctcgc gaaacacacg gcccgctgtt ccgctacacc tatgccgact
     8641 gcgccaagcg cgcgcgcaag ctggcgcatg cgctcggcca gctcggcctt tccggcggca
     8701 gcgcggtcgg ctcgatcgcc tggaacaacc accgccacct ggaggcgtac tacgcggtct
     8761 ccggcagcgg catggtgatg cacacctgca acccgcggct gcacccgcag cagctgatct
     8821 acgtcatcaa ccatgccgaa gaccaggtga tgctgttcga cgccaccttc gcaccgctga
     8881 tcaagggcat cgcgtcgcat tgcccgaagg tcaaggcctg ggtctgcctg gccgacaagg
     8941 cgaacacgcc ggccatcgag ggcgtggcca atgtgctgag ctacgaggag ctgatcgcgc
     9001 cgcacagcga gcacttcgaa tggccccagt tcgacgagcg cagcggtgcg gcactctgct
     9061 acacctcggg caccacgggc aacccgaagg gcgcgctgta ctcgcatcga gcgatcgtgc
     9121 tcaacgcgct ggtcggcagc acgccgggcg tgctggcgtt gtcggcgcgc gagacgatcc
     9181 tgccggtggt gccgatgttt cacatcaacg cctggtgcat cccctatggg gcgccgatgg
     9241 tcggcgccag gctggtgctg ccggggccga agctggacgg cgccagcctg tacgagctga
     9301 tggagagcga gcgcgtcacg gtcagcgccg gcgtgccgac catctggctg gcgctgctgc
     9361 agcatgtgga agcgaacaag ctgcgcttca cgacgatgcg ccgtacggcg gtcggcggct
     9421 cggcgatgcc gctgtcgctg atcgccaagt ttgccgacga gtacggcgta gaggtacggc
     9481 acggctgggg catgaccgag accactgcgg tggcgacgat gagcgcgctg atgagcaagc
     9541 acgatccgtt gtccgccgcc gacaagcatg cggtggtcgc caagcagggc aaggctgtgt
     9601 tcggcgtgca gatcaaggtg gtcgacgaga cgggcgccac gctgccgcgc gacggcagtt
     9661 cgcagggcga gctgatggtg cgcgggcagt ggatcgtctc cggctacttc aagggcgaaa
     9721 gctcgccgct gcaggacggc tggttcccga ccggcgacgt ggcgaccatc gacgccgatg
     9781 gcgtgatgca gatccgcgac cgcgccaagg acgtgatcaa gaccggcggc gagtggatca
     9841 gttcgatcga cctggaaaac gccgcgatgg gccaccccgg cgtagcgatg gcggcggtga
     9901 tcggcgtcaa gcacccgaag tgggacgagc gcccgctgct gttcatcgtg cgcaagccgg
     9961 gccaggcgct ggagaaggac gagatcctcg ccttcttggc cgagcgcgtg gccaagtggt
    10021 gggtgccgga cgacgtggtc ttcgtcgact cgctgccggt gggtggcacc ggcaaggtgc
    10081 agaaggccga cctgcgcaag cagtacggcg gcgtgttcag ttgacgggag gcggtcatgc
    10141 ctatcgaacc tgctgaagac cgcggcctgc cgcggcgcga ctttctcaag gttggccttg
    10201 gcttctcgct ggcgctcacg gtgggcacgg ccgtcgtcgg ctgcagcgac gcggccaaga
    10261 cgctatcgac cggctatgtc ttcctgcgtc ctggcgacgt ggaactgttc agcgcgctga
    10321 cgccggcggt ggtcaccgaa ctcggtgcct tcgatgcggc caagcgcagc aagctggtcg
    10381 agcgcacgct gcagaacatc gacggcacct gcgccgcgct cgatctgggc agccggcagg
    10441 aactgcgcaa gctgctcgac ctgctggcca tcggcccgct gcgctactcc ctgacgggcg
    10501 tgggcgcgtg gaacgaggcc ggcatcgaca agatgcaggt cttcttggcc cgctggcgcg
    10561 gcagccgctt cgccactctg aacgccggcg gcaacgtgct ggtcaagctc acctcttcga
    10621 gcttctacgt gttgcccgag agctggccct ccaccggcta cccggggccg ctggaatacg
    10681 tgttcaacgc gatcaactcc tgaaagcctg ccatggccct acctgacatc tacaccgaag
    10741 gcattgcctc gggctggaag gtcgtcgacg cggccaagct gacggccccg cagacgatgg
    10801 aggccgacgt agccatcatc ggcagcggcg ccggcggcgg cgtggccgcc gagatcctcg
    10861 ctctcgccgg actgaaggtg ctgcttctgg aagagggcgc attacgcacc tcggacagct
    10921 tcaaggacat ggacgagggc cgcgccctgc gcgagctgta ccaggaaggc gccgcacgtg
    10981 ccacgtccga cggcgccatc gccgtgctgc aggggcgctc ggtcggcggc tcgaccaccg
    11041 tcaactggac ctcgagcttc cgcacgccgg agccgacgct gaagttctgg gccgccgaac
    11101 gggaggtggt cggccatggc gtggacgaca tgaaaccctg gttcgagaag atggaggcgc
    11161 gactggacgt catgctgtgg gaggcgcaga acgccaacaa cagtgtgctc aagcgcggct
    11221 gcgacaagct cggctgggag gcgcactcga tcccgcgcaa cgtgcgcggc tgctggaact
    11281 cgggctactg cggcttcggc tgcccggtca atgccaagca gtcaatgctg gtgtcgacga
    11341 ttcccgaggc gctgcgccac ggcgcgacgc tggtgcaccg gctgcgcgtg cgccaactgc
    11401 agcatgccgg cgacaagatc accggcgcgg tcggcgaggc actgcgcgag gacggccgca
    11461 cgcccagcgg cacgctggtg acgatcaagg cgcggcattt catcgccgcc ggcggcgcca
    11521 tcaacacccc cgcgctgctg ctgcgatcgc aactgcccga cccgcacaag cggctgggcc
    11581 agcacacgct gatccacccg gtggtgctgt cgctggcgca gatgcccgag cgcatcgacc
    11641 ccttctacgg cgcgccgcag tccacggcct ccgaccactt ccagtggaag gacggcgcca
    11701 ccgggccgat gggctacaag ctcgaggtgc cgccgatctt tcccggtatc ggcgccggcg
    11761 tgctcaacgt ctacggcgac gatctgcgca aggaaatggc tgcgttgccg cacaccaacg
    11821 cggtgctggc gctgctgcgc gacggctttg tcgaacagag ccccggcggc agcatccgcc
    11881 tggccgacga cggcagcccg atgctcgact acgagctcag cgactatgtc tgggaaggcg
    11941 tgaagcgcgc ctacctgagc atggctgaac tgcagttcgc ggccggcgcc cagcgggtgc
    12001 gccaggcgca catcgatgcg agctaccaga ccagctggac cgatgcgaag aaggcgattg
    12061 ccgagctgcc gttgaagaag ttccgcgcca cgctgttcac cgcacacctg atgggcggct
    12121 gcgcgatgag ccagaacccg aagcgcggcg tcgtcgacag cggcggccgg catcatcaat
    12181 tggccaacct gtcggtgatg gacggatcgg tgttcccgac cagcatcggc gccaacccgc
    12241 agttgtcgat ctatgggctg gtggcgcaaa acgccacggc gctggccaaa caattgaaga
    12301 cctgaagtga gcgaagctgc accgcccctg gcggccgtgc cgcgcgcggc ggccgtggcc
    12361 ggcgtggcct ttgcgctgtt gttcggcgcg gcgctgctgt tggtgcggct gtcgattccg
    12421 ggctcgatcg tcgagcgcgg cggctggctg gccgacccgg ccggaaattt gcgcctggcc
    12481 accgacctgc tggcctgggg cggcatcgcc ttcctgtggt tcatcggcgt ggtgcgcacg
    12541 cacatcggcg cgcgcgagga ccgcttcctc gcatcggtgt acttcggcag cggcgtgctg
    12601 ttcctgggca tgagcttcat tgctggcgcc acggcgcagg cgctgctggc ggcgcacgca
    12661 cagtacggcg agcgcttctt cgacagcggc accttcgcct tcggcggtcg gctggcctac
    12721 gagctgacca acacctatgc gctgcgcatg gccggcgtgt tcatgttttc gctggccacg
    12781 ctgtgccatc gcaccggcgc catgccacgc gtgttcgtct gggccacctg gaccctggcc
    12841 gcgctgctgg tgctgggcct gacgcaaacg ctgtgggcgg cgttgctgtt cccggcgtgg
    12901 gtgctgggtt tcagcctgta cctgctgctg cggccgggcc cggcgcaggc gtgatctttg
    12961 tcttccgcga cgttgatcgg tgagcgatgc cgtagcgggg atcggagagt cttgtgctcg
    13021 tgctgctcgt gcaccacgtt cggcgcaggc aggtcgtcct ggtcgggaaa caccactgca
    13081 tgcgcgatgt tcatacggta cagagcatgg ctgggcgggc ggacggcatg aagcggccct
    13141 cctgacccgc caacgagacg gtcctatcgg tcagctgttc agctgcaagt gttcaagacc
    13201 ctgagctgtt caataagccg attggcggtc gatgttctga accgccagag agcaacaagg
    13261 cggtctaccg ccagtacccg ctcatggccg agtggagccg accggttttc gccggcgaac
    13321 ggccggtgta catcaggtt
//
LOCUS       DAOORV010000102        12677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6123_length_12677_cov_6.091269, whole genome shotgun sequence.
ACCESSION   DAOORV010000102 DAOORV010000000
VERSION     DAOORV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6123_length_12677_cov_6.091269"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PKV56_13015"
     CDS             complement(<1..1264)
                     /locus_tag="PKV56_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX68778.1"
                     /translation="MSTWERFLSGEVCGSDAIRRLIDDSWRRCQSAQVDPGRDKAPPA
                     LAEDALHTLLSEHDELLNAGTSVMAHARQFLDETGTVMVLTTNDGVVLNLEGDPATSA
                     AAAERIRMIPGSSWSEATVGTNAIGTALAIGQPVQIHSAEHYCAGIKRWTCSATVVRD
                     PYNNSILGAIDVSGLNATYNRHSLALVVTTASRIESRLARREMELRYRLLELCVSRLG
                     RSTNDGVILLDRRGNPIKVSDKAQAALSALNAAGKSTSPINLSTLKIPTSFKSAAAEN
                     LPDWISRDWLEPIVENGQKLGSILTIPNRSTPVTLRSVETISAKSTCLKNEAFERVIG
                     KAPKLLEVIECAQRLSKSSVPLLLLGETGVGKDVFARAIHETSAAREAPFVALNCGGF
                     SRELLTSELFGYADGSFTGARRGGMIGKI"
     gene            complement(1634..2812)
                     /locus_tag="PKV56_13020"
     CDS             complement(1634..2812)
                     /locus_tag="PKV56_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOX68779.1"
                     /translation="MKAMNVSIVGWAQTPFGKFDNESVESLIVKAVNGALESAGISAA
                     DVDEIYLGHFNAGFSKQDFTASLVLQANDALRFKPATRVENACATGSAAIHQGVKAIE
                     AKRARIVLAVGVEQMTPTPGPEVGANLLRASYLREESGIQGGFAGLFGQIATSYFEKY
                     GDQSDALAMIAAKSHRNGSFNPYAHFRKDLGYDFCRQVSDKNPFVAGPLKRTDCSPVS
                     DGAAAVVLADAETAATLTRAVAFRGLAHVQDYLPMSRRDILKLEGCALAWRKAFDSAG
                     VQLSDLSFVETHDCFTVAELIEYEAMGLAPEGQGAVAIREGWTQVGGKLPVNASGGLK
                     AKGHPVGATGVSMHVMTAMQLNGELPAEITVKGARLGGIFNMGGAGVANYVSILERVR
                     "
     gene            complement(2846..4345)
                     /locus_tag="PKV56_13025"
     CDS             complement(2846..4345)
                     /locus_tag="PKV56_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX68780.1"
                     /translation="MNLFSLLDQTATRYPDLGAVFHGIDQVLTWSELRSRALCLGSGI
                     RKRCGTGERIALVSENRPEYVELMFSAWAAEAAIVPINYKLHDREVAQILEDAEVSLV
                     FVSPTLLPGLQRTAAAYPGRIVVIAEAEYAALLATEPLSPPDSDPQALAWLFYTSGTT
                     GRSKGAMLSHRNLLAMEIAHLADIEVMNENTSQIHVAPMSHGSGLYILPSVARGARQV
                     VPASGGFDPVEFLDLCDQHPSCGSFLAPTMVQRLRLTVESGKRGASKNLRCIVYGGGP
                     MYVDELRKSTVALGPVFAQIYGQGESPMTITGLDRAGHAIDDDAILGSVGWPRSGVEV
                     RVIGPDGAAVPAGAVGEIVCRGDVVMAGYWRNPTATAQALQGGWLRTGDMGSFDERGC
                     LTLRDRSKDVVISGGSNIYPREVEEALLAHAGVIKTAVVGRPDPEWGEVVIAFVVCAP
                     GANVTAADLDAHCLEYIARFKRPKEYRFLDELPTSSYGKVLKNELRKML"
     gene            complement(4796..7351)
                     /locus_tag="PKV56_13030"
     CDS             complement(4796..7351)
                     /locus_tag="PKV56_13030"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOX68781.1"
                     /translation="MSVDSSEPSPAGLTRGEAARRLEADGPNDLPRAPQRTTWRIAIE
                     VASEPMFQLLAAAVAIYLVLGDVSEASVLFAMLGVIVATELVQERRTEKVLEALHDMT
                     SPRALVWRDGRRERIAGVDVVLGDLIELSEGDRVPADARLLRSNDLLIDESLLSGESL
                     PVAKRAASGQDAGSSAADPSIVYAGTMVVAGQGLAEIVATGARSEIGRIGKVLGAIEA
                     EPTPLHRQTRQLVRWFSVLGLAVSLVVAALYVWTHDDWLGGALAGITMAMSMLPQEFL
                     LILTVFMAMGAWRLSQHRVLTRRSATIEALGSATVLCTDKTGTLTRNQMAVGALVLFD
                     GKVPVCWTATNDPLPQAFHDLLHHGVLASEERPFDPMEQAIQTLGARCLSPVASLELV
                     HEYGLSPDLPVMSHVWSTGDGLQHRIAAKGAPESLASLSHLAPDQREVVLDEAARLAD
                     LGMRVLGVASAEWGTAAWPAEQSGFEFRFLGLVGLADPLRENVPQAVSECRQAGIRVV
                     MITGDHPGTARAIARQAGIDGDSGVLLGAQVAALSDPDLRLAVATTTVFARVAPQQKL
                     RIVEALKANGEVVAMTGDGVNDAPSLKAAHIGIAMGGRGTDVARESASLVLLDDDFGT
                     LVQAVRLGRRIFDNLRKAMRFVFAVHVPIAGLTLLPLMFGWPLLFTPMHIAFLEIAID
                     PVCSIVFEAEDEEPDLMTRPPRDTMAALFSPLLIAASLLQGVLVLVAVGFFYWILLHE
                     GVPEGQSRASAFVALVTSNLALILVNRSSRSGLRALLMTRNRALWLTLLATAAMLSAV
                     VGIEVLRKLFGFELPMSSMLVSSVLVGLAVGPLLLIQRAAGRRMAVLLGWRSD"
     gene            complement(7388..11569)
                     /locus_tag="PKV56_13035"
     CDS             complement(7388..11569)
                     /locus_tag="PKV56_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX68782.1"
                     /translation="MIPGSTPSPGASSGMGPGRPRARQQWLQLGAVLIAVGLFAIWAR
                     YSDYREVDDEQRHLLVVQARGVDEALSRQLAGVGAALRDLRDDLARRPAGEVEAGVSS
                     RLQALGNAMPGVGSLVLLDRSGRAVAANRSDLLGRDLSDRDYFQLARDQSDPKRLSLS
                     PPFMGSLGTYSMNLSLPVRSADGAFAGVVTSMLDPDYFRSAIRPTLYAADSRGAVIHA
                     DGRLLMFEPRSDKAVGMNVDTPGSFFRRHVASGQLESLLTGVCPVTGDKRMVALRTVR
                     PSDLYVDKPIVIAIGRNLDAMFATWWRQTLVYGLLYSVFAAVTSSALVLVQRRQRAID
                     FLNAQRKESEHQSARRLELALEGADLGLWDFDVPSGATLVSERINSMLGLPHQNYPDS
                     QAWRSRVHPDDLERVREAKLEHLEGRAEHFDQTYRMLHADGHWVWVLDRSRVFERDAQ
                     GKPLRMVGTHMDVTEQVEARLALQRSEQSLSTTLYSIGDAVVATDAQGRIVRMNATAE
                     RLSGWPAPEAIGQQLTAVFRIVNARTGELSIDPARQVIERGEVVGLTNDTVLLARDGR
                     EYNISDSAAPIRTPEGTITGVVLVFSDISERYRAQQVLRANEERLRTLLDNLRSGVVI
                     HAADTRVVDANASACRMLGLTIEQLRGRDAADPHWTFLEEDGQPMTIDRYPVNQVRAS
                     GEPLKNFMVGFLRSDLESPLWALCNAFVLRNALGDIENIVVTISDITERMEAELLLRE
                     SEALRRSVLDSLNAHIAVVDRDGAIIAVNEAWRRYGQDHGVSARFIEPIGVSYLNLCA
                     DSGAAAPASASASEAVAALDGIRSVLAGFEEEFHLEYPCNSATETLWFRMSVTPMHGS
                     RGGAVIAHMPVTELKQAEQNISAARAELQATLEAVPDLLFEIDREGRFHNFHSPRHER
                     LHVSPDAFIGRVASDVLPTEVVDVIRRAIAQAEAAGYSSGYVYELALPGGPHWFELSV
                     ARKPMPDGEVPRFIALARDISERKLSELEREALESQLREAQKMESIGTLAGGIAHDFN
                     NILAAILGNVALAREDVAAGHSVLASLEQINRAGLRARHLVKQILAFSRREQHEFSLQ
                     PLGPVIAETIDMLRATLPAGVRLDTVVPELPVEVRGDSTQLQQVLLNLCTNAWQALPE
                     QGGRIEIGFERMMLDEAQRQRQRVTDLPPGTCVHLWVRDNGCGMDEATRLRIFDPFFT
                     TKKVGRGTGLGLSVVHGIVRAHQGAIGLDTVPGQGTTFHVYLPNPERRAAERPVEPDS
                     IAGARGSGQHVLYIDDDEVMVLMVERLLKRAGFRVTVETRAAAALELVRAQPQDFDAV
                     VSDFNMPELSGIDVASQIACIRPDLPVVISSGLLTDELRSQAEQLGVRALMHKENTFE
                     ELASLLQRLLAPAAS"
     gene            complement(11696..>12677)
                     /locus_tag="PKV56_13040"
     CDS             complement(11696..>12677)
                     /locus_tag="PKV56_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX68783.1"
                     /translation="LVTTSELYQTRVAGLRHRLPKLKHVIVVGAGFAEGAGVRRWGDV
                     VEGASHRFTTAATDTETVALLHFTRTAEGRTQTTIHVHGAVLAHAVTGHYALDFHDDD
                     IFWCTADPGCVTGTSYGIIAALVAGVTSVVVEAEFDPATCCALLEQERVSVWYTAAPE
                     IRDLMDTDAVAVRSHDLSALRFMASVGEPLTADAVIWGIEVFGLPFHDNWWQTESGAI
                     MIANYAAMDIKPGSMGKPLPGIRAAIVRRDAQGRVETITRPDVEGELALEAPWPSMMR
                     GYLHDEERYRNRFCDGWYLSGGMARCDADGYYWFVRHAGDAIDLASNTIA"
BASE COUNT         2370 a         4275 c         3955 g         2077 t
ORIGIN      
        1 cgatcttgcc gatcatgccg ccacgcctgg cgccggtgaa tgatccgtcg gcgtaaccga
       61 acagctcgct ggtcaacaat tcgcgcgaga agccgccgca gttcagagcg acaaacggcg
      121 cctcccgcgc agcgctggtc tcgtgaatgg cgcgcgcgaa cacgtccttg cccacgccgg
      181 tttcaccgag cagcaggagc ggcacgctcg acttggagag ccgctgggcg cactcgatga
      241 cctcgagcag cttcggcgcc ttaccgatga cacgctcaaa agcctcgttc ttcaagcagg
      301 tggacttggc gctgatcgtc tccacgctgc gcagcgtgac cggggtgctt cggttcggaa
      361 tggtcaggat agagcccaat ttctgaccgt tctcgacgat gggctccagc cagtcacgac
      421 tgatccaatc aggaaggttc tcggcagcag cgctcttgaa cgaagtcggt atcttgagag
      481 tcgacaggtt gatgggcgat gtcgactttc ctgcggcatt gagggcactg agcgccgcct
      541 gggccttgtc cgacaccttg atcgggttcc cacgacggtc cagaaggatc acaccgtcat
      601 tcgtcgaccg ccccagtcgc gaaacacaca gttccagcaa tcggtaacgc aactccatct
      661 cgcggcgagc cagccggctc tcgattcgac tggccgtggt gacgaccagt gcgaggctgt
      721 ggcggttgta ggttgcgttc agccctgaaa cgtcgatcgc tccgaggatg ctgttgttgt
      781 aaggatcccg gaccaccgta gccgaacatg tccagcgctt gatcccggcg caatagtgtt
      841 ctgccgaatg gatctggacc ggctgcccga tcgccagcgc ggtaccgatg gcattggtgc
      901 cgaccgtggc ctcgctccag ctgctgccgg gaatcatgcg aattctttcc gcggcagcgg
      961 cagaagtcgc agggtcgcct tccagattga gaacgacacc atcgttcgtg gtcaacacca
     1021 tgacggtgcc cgtctcgtcg agaaactgcc gggcatgcgc catgacggag gtgccggcgt
     1081 tcagcaactc atcgtgttcg ctgaggaggg tatgcagcgc gtcttcagcc agtgcaggag
     1141 gcgccttgtc acggcccggg tcgacttgcg cgctctggca acgccgccac gagtcgtcga
     1201 tcaagcgccg aatcgcgtcg gatccacaca cctcgccact caggaaccgc tcccaagtcg
     1261 acatgatgtg cgtgtcgttc tctggacgag aaaatatctg acttggaagg ggactacgca
     1321 taccgggctc cggaatactc gttaagtcgg cgaatcgtgc aagtcatgtc tgcaaagggc
     1381 gctcgaaact gacacctgtc aacaaaccgc gcgccctgca ggacgacggg aaggctgtgt
     1441 agcgacgcaa ccgctccaat ccgaagcaaa aacccggcgt ttatggagcg ggagaggaaa
     1501 tgtaagcgtc aagtgcgggg agtcaaccga ggaaattccc tcgtaggagc ggcgaactgc
     1561 tcatcagttc aggaaaacga gggggccttc gaaagtcgcg accatgaact gcgcccagca
     1621 gaccggccga gggtcagcgg acgcgctcca ggatgctgac gtagttggcc actccggccc
     1681 cgcccatatt gaagatgccg cccaacctgg cgcccttcac cgtgatctcg gccggcaact
     1741 cgccattgag ctgcatggcg gtcatcacgt gcatcgacac cccagtcgcg ccgaccgggt
     1801 gtcccttggc cttcaacccg ccggaggcgt tgaccgggag ttttccgcca acctgggtcc
     1861 agccttcgcg gatcgcgacg gcaccctgcc cttcaggcgc cagccccatg gcttcgtact
     1921 cgatcagttc agccacggtg aagcaatcat gcgtctcgac aaacgacaga tcgctcagtt
     1981 gaacgcctgc cgaatcaaac gccttgcgcc aagccagggc acagccttcc agcttgagaa
     2041 tgtcgcgccg ggacatcggc agatagtcct ggacatgcgc cagtccccgg aaggccacgg
     2101 cacgggtcag cgtggccgcc gtctccgcat cggccagcac caccgcagcg gcgccatcgg
     2161 aaaccggcga gcaatcggtg cgcttgagtg ggccggcaac gaacgggttc ttgtcgctga
     2221 cctggcggca gaagtcgtat ccgagatcct tgcggaaatg ggcatagggg ttgaacgaac
     2281 cgttcctgtg actcttggcg gcgatcatcg ccagcgcgtc ggactgatcg ccgtacttct
     2341 cgaagtagga ggttgcgatc tgcccgaaca gcccggcaaa tccgccctgg ataccgcttt
     2401 cctcgcgcag gtaggaggct cgcaacaggt ttgcgcccac ctccggaccc ggcgtcggcg
     2461 tcatctgctc cacgcccacc gccaggacga tccgggcgcg cttggcttcg atcgccttga
     2521 cgccctgatg aatggccgcc gagccggtgg cgcaggcgtt ttcgacccgc gtggcaggct
     2581 tgaagcgcaa ggcgtcattg gcctgcagca ccagcgacgc ggtgaagtcc tgcttcgaga
     2641 aacccgcgtt gaagtgcccg agatagattt catccacgtc ggccgccgaa atacccgctg
     2701 actcgagggc accattcacc gccttcacga tcaagctctc gacgctttcg ttgtcgaatt
     2761 ttccaaacgg ggtctgagcc caaccgacga tagatacgtt catggccttc atccttttgt
     2821 tgatgcaacc tggagccgag ccggctcaga gcattttgcg gagttcgttc ttcagcacct
     2881 tgccgtaact gctggtcggc agttcgtcga ggaatcggta ctccttcggg cgcttgaacc
     2941 gggcgatgta ttccaggcag tgcgcatcca gatctgcggc ggtcacgttg gcacccggcg
     3001 cacacacgac aaaggcgatc acgacttcac cccactccgg atccggacgg cccaccaccg
     3061 cagtcttgat cacaccggca tgggccagca gtgcctcttc cacctcgcgc ggatagatat
     3121 tgctgccacc actgatgaca acgtccttgg agcgatcgcg caacgtcagg cagccacgct
     3181 catcgaagga gcccatgtcg ccggtgcgca gccatccccc ctgaagggcc tgcgcggtcg
     3241 ccgtcggatt gcgccagtaa ccggccatca ccacgtcgcc tcgacagacg atctccccta
     3301 cggctcccgc tgggaccgcc gcgccgtcgg gcccgatgac acgcacctcg acaccgctgc
     3361 ggggccagcc cacggaaccc aggatcgcat cgtcatcgat ggcatgcccg gcacggtcga
     3421 ggccggtgat cgtcatgggc gactcgccct gcccgtaaat ctgtgcgaac accggtccca
     3481 gcgccaccgt ggacttgcga agctcatcga cgtacatcgg gccgccgcca tagacgatgc
     3541 agcgcagatt cttcgacgca ccgcgctttc cggattcgac ggtgagccgc aggcgctgga
     3601 ccatcgtcgg cgccaggaac gatccgcagg agggatgctg gtcacacagg tccaggaatt
     3661 caaccggatc gaatccgcca gacgccggca cgacctgcct cgcaccacgc gcaaccgacg
     3721 gcaggatgta gagcccggag ccgtgggaca tcggcgccac gtgaatctgg ctcgtgttct
     3781 cgttcatcac ctcgatgtcg gcgaggtggg cgatctccat cgccagcaga ttgcggtgcg
     3841 acagcatggc ccccttggag cgcccggtcg tgccgctggt gtagaagagc caggcaaggg
     3901 cttgcgggtc gctgtccggt ggcgacaggg gctcggttgc cagcagagcc gcgtactcgg
     3961 cttcggcgat gacaacgatg cggccgggat aagctgcggc ggtccgctgc aaccccggca
     4021 acaaggtggg cgacacgaac accagcgaaa cctcggcatc ctcaaggatc tgggctactt
     4081 ccctgtcatg cagcttgtag ttgatcggca cgattgccgc ttctgcagcc caggccgaga
     4141 acatcagttc gacgtattca ggacggttct cgctcaccag cgcgatgcgc tcacccgttc
     4201 cgcagcgctt gcggatgccc gagccgaggc acaaggcacg agacctcagt tcactccacg
     4261 tcagcacctg gtcgatgcca tgaaacaccg cgccgaggtc cgggtagcgg gtggcggtct
     4321 ggtcgagcag agaaaaaaga ttcattcaaa ggcatccaat gcgagctgtc actcggccag
     4381 cgggaaaact gtcgaccacg gggcctcggc ggacctcgtc atcacagcca ttttgcaaat
     4441 accgggtgga aatggcccga tcgtcggcat gacgaaattc gggacccctc catttttgca
     4501 ttatgaatta cgcaactcca gtgccatcca ggcaacgcgc cgctgtcacg ccctacaccc
     4561 ttgatgcgga tcaatatttt aggtctcgat gaccaaccgc ccggacgtcc gatcggacag
     4621 aaccccggag aagacccggt caatcgaacg gaatccggac aggcagaccg accacctgcg
     4681 tcccaggtta agccgccaga gacgcgggga tgacgcgcct ctgcccgccg catcgtgcga
     4741 aaacgacgcg atatcgcgca gcggcaaggc ttcgccccgg tcgaaactgc cgggatcaat
     4801 ctgaccgcca tccgagcagg acggccattc gccgccccgc agcccgctgg atcagcagca
     4861 acggaccgac agccagcccg accagcaccg atgagacaag cattgaggac atcggcaatt
     4921 cgaacccgaa cagcttgcgc agcacctcga tgcccaccac ggcagacagc atggccgcgg
     4981 tggccagcaa tgtgagccag agggcgcgat ttcgggtcat cagcagcgcg cgcaagcccg
     5041 agcgcgacga gcggttcacc aggatcagag ccaggttgga agtgacgagc gcgacgaagg
     5101 ccgaggcgcg agactggccc tccggaacac cttcgtgcag caggatccag tagaagaagc
     5161 cgacggccac cagcacaaga acaccctgca gcagactcgc cgcaatgagc agcggcgaga
     5221 aaagcgcagc catcgtatcg cggggcggcc gggtcatcag gtcgggctcc tcgtcctcgg
     5281 cttcgaagac gatcgagcac accggatcga tcgcgatctc gaggaatgcg atgtgcatcg
     5341 gcgtgaacag caacggccag ccgaacatca gcggcagcag cgtgagaccc gcgatcggta
     5401 cgtgcaccgc gaagacgaag cgcattgcct tgcgcaggtt gtcgaagatg cgccgaccca
     5461 ggcgaacggc ctggacgagc gtgccgaaat catcgtcgag cagcacaagc gacgccgact
     5521 cgcgcgcgac atcggttccc cgcccgccca tcgcgatgcc tatgtgcgca gccttcagcg
     5581 atggcgcatc gttgacgcca tcgccggtca ttgcaacgac ttcgccgttg gccttcagcg
     5641 cctcgacgat ccgaagcttc tgctgaggag ccaccctcgc aaacacggta gtggttgcca
     5701 ctgcgagccg aagatcgggg tcgctcagcg cggcaacctg cgcgccaagc agcacgcccg
     5761 agtcaccatc gatgcctgcc tggcgggcga tcgcgcgcgc ggttccaggg tggtcaccgg
     5821 tgatcatgac gacgcggatg ccagcctggc gacactcgct caccgcctgc ggaacgttct
     5881 cgcgcagcgg gtcggccagg cccaccaggc ccagaaagcg gaattcgaag ccactctgct
     5941 ctgctggcca ggcggccgtt ccccattcgg ccgaggccac gccgagcacc cgcatgccga
     6001 ggtcggcgag gcgcgcagcc tcgtccagca cgacttcgcg ttgatcaggc gccagatgac
     6061 tcagcgacgc caggctttcg ggagccccct tggcggctat gcgatgctgc aggccatccc
     6121 ccgtgctcca cacatgcgac atcaccggca agtcgggcga caggccgtac tcgtggacca
     6181 attccaagct ggcaacgggc gaaaggcaac gggcacccag agtctgaatc gcctgctcca
     6241 tcggatcgaa cggacgctcc tcgctggcca gcacaccgtg atgcagcaga tcgtgaaacg
     6301 cctgcggcaa cggatcattg gtggcggtcc agcagaccgg aaccttgccg tcgaaaagaa
     6361 ccagagcgcc gacggccatc tggttgcgcg tgagcgttcc ggtcttgtcc gtgcacagga
     6421 ccgtggccga acccagcgct tcgatcgtcg ccgaacggcg cgtcagcacc cggtgctggg
     6481 aaagccgcca cgctcccatc gccatgaaga cggtcaggat gagcaggaac tcctgaggca
     6541 gcatcgacat cgccatggtg atgccggcca gcgcgccgcc cagccagtcg tcgtgcgtcc
     6601 agacgtagag agccgccacg accagactga ccgcgagccc gagcacggag aaccagcgga
     6661 ccagctggcg cgtctggcga tgcagcggcg tgggctcggc ctcgatggcg ccgagcacct
     6721 ttccgatgcg gccgatctcg ctgcgcgcac ccgttgcgac gatctcggcg aggccctgcc
     6781 ctgccacgac catggtgccg gcgtagacga tcgacggatc ggcagcggac gatcctgcgt
     6841 cctggccgct cgcggcgcgc ttggccaccg gcagcgattc gccgctcaac aacgattcat
     6901 cgatcagcag atcgttcgac cgaagcagcc tcgcatcggc aggcacacga tcgccctcgg
     6961 acagctcgat gaggtcgcca agaacgacgt ccacaccggc aatgcgttcc cggcgaccat
     7021 cgcgccagac cagtgcacga gggctggtca tgtcgtggag agcctcgagc accttctcgg
     7081 tccggcgctc ctgcaccagc tcggtcgcca cgatcacacc gagcatggcg aacaggacgc
     7141 tcgcctcgga cacgtcgccc agcaccagat agatcgccac cgccgccgcc agcaactgga
     7201 acatcggctc gctggccacc tcgatcgcga tgcgccaggt ggtccgctgc ggtgcccgcg
     7261 gcagatcgtt ggggccatcg gcttcgaggc ggcgggcggc ttccccacgg gtcaggccag
     7321 ccggcgacgg ctcacttgaa tcgacgctca tcgagccgag tccatcgggt gtcgggccat
     7381 gctgggttca ggacgctgct ggcgcaagca atcgctgcag caggctggcc agctcttcga
     7441 aggtgttttc cttgtgcatc agtgcgcgca cgccgagttg ctctgcctgg gagcgcagtt
     7501 catcggtcag caggccactg ctgatcacca ccggcaagtc gggccggatg cacgcgatct
     7561 ggctggcgac atcgatgccc gacagctcgg gcatgttgaa gtcggagacc acggcatcga
     7621 agtcctgtgg ctgcgcgcga acgagctcca aggccgcggc agcacgggtc tcgacggtca
     7681 cacggaaccc cgccctcttc agcaaacgct cgaccatcag gaccatgacc tcgtcgtcgt
     7741 cgatgtagag cacgtgctgc cccgaacccc gtgcacctgc gatcgaatcc ggctccaccg
     7801 gccgctcggc ggcgcggcgc tccggattcg gcagatagac atggaatgtc gttccctgcc
     7861 ccggcacggt gtccagcccg atggctccct gatgcgcgcg cacgatgccg tgaaccaccg
     7921 acagtccgag cccggtaccc cgtccgacct tcttggtggt gaagaacggg tcgaagattc
     7981 gcaggcgggt tgcctcatcc atcccgcagc cgttgtcgcg cacccacaga tggacgcagg
     8041 tcccgggcgg caggtcggtc acgcgctgac gctgtcgctg cgcctcgtcg agcatcatcc
     8101 gttcgaaacc gatctcgatg cgaccaccct gctcgggcag cgcctgccac gcattggtac
     8161 acaggttcag cagcacctgt tgcagctggg tgctgtcacc gcgaacctcg acaggaagct
     8221 cgggcacgac ggtatcgagg cgcacaccgg ccggcagcgt ggcccgcaac atgtcgatcg
     8281 tttccgcgat caccggaccg agcggctgca gcgagaactc atgctgctcg cggcgactga
     8341 atgcaaggat ctgcttcacc aggtgccggg cacgcaggcc agcgcggttg atctgttcca
     8401 ggctggcaag caccgaatga cccgcagcca cgtcctcgcg ggccagcgcc acgttgccga
     8461 gaatggccgc caggatgttg ttgaaatcgt gcgcgatgcc gcctgccagc gtgccgatcg
     8521 actccatctt ctgggcttcg cgcagctggc tttcgagcgc ctctcgttcg agctccgaca
     8581 gcttgcgttc gctgatgtcg cgagccagcg cgatgaagcg cggaacctcg ccatcaggca
     8641 tcggcttgcg agcaaccgac aactcgaacc agtgcggccc gcccggcaag gccagttcgt
     8701 agacataccc cgacgaatag ccggcagctt cggcctgggc gatggcacgg cggatcacgt
     8761 cgacgacttc ggtgggcaga acatccgaag caacgcgccc gatgaacgca tccggcgaca
     8821 cgtgaagcct ctcgtggcgc ggcgagtgga agttgtggaa tcgcccctcg cgatcgatct
     8881 cgaacagcag atcaggaacc gcctcgaggg tggcctgcag ctccgcgcgc gctgcgctga
     8941 tgttctgctc ggcctgcttg agttcggtca cgggcatgtg ggcgatgacc gcaccaccgc
     9001 gggaaccatg catcggtgtc acgctcatgc ggaaccacaa ggtctccgtc gccgaattgc
     9061 acgggtactc cagatggaac tcctcctcga agccagccag caccgaacgg atgccatcga
     9121 gtgccgcgac tgcctcgctg gcagacgcgg aagcgggcgc agctgcgccc gagtcagcac
     9181 acagattcag gtagctgacg ccgatcggct cgatgaatcg ggcagacacc ccgtggtctt
     9241 gcccgtacct gcgccaggcc tcgttgacgg caatgatcgc gccgtcccga tcgacgacgg
     9301 cgatgtgggc attgagcgaa tccagcaccg agcgcctgag ggcttcgctt tcgcgcagca
     9361 gcaattcggc ttccatccgt tcggtgatgt cggaaatcgt cacgacgatg ttctcgatgt
     9421 ctcccagggc attccgcagg acaaaggcat tgcagagtgc ccacagcggg ctctccaggt
     9481 cggaccgcag gaatccgacc atgaagttct tcaagggctc gccgctggcc cgaacctgat
     9541 tgaccggata ccgatcgatc gtcatcggct gtccgtcctc ttccaggaag gtccagtggg
     9601 gatccgccgc gtccctgcca cgcagctgct cgatcgtgag gccaagcatg cggcacgccg
     9661 atgcgttggc gtcgacgacc cgggtatccg ccgcatggat cacgacgccg gaacgcaggt
     9721 tgtccagcaa cgtgcgcagt cgctcctcgt tcgcacgcaa gacctgctgc gcgcgatagc
     9781 gctcgctgat gtcgctgaag accagcacca ccccggtgat cgtcccttcc ggggtgcgaa
     9841 tcggcgccgc gctgtccgag atgttgtact cgcggccatc gcgagcgagg agcacggtgt
     9901 cgttggtcag accaacgact tcaccgcgct cgatcacctg ccgggccggg tcgatggaca
     9961 actccccggt gcgcgcgttg acgatgcgga acaccgccgt cagctgctgg ccgatggcct
    10021 ccggggcggg ccagcctgac agtcgctcgg cggtcgcgtt catccgcacg atgcgtccct
    10081 gcgcatcggt tgcgaccacc gcatcgccga tcgaatagag cgtcgtggac aggctctgct
    10141 cgctgcgctg cagggcgagt cgggcttcca cctgctcggt gacgtccatg tgggtgccga
    10201 ccatgcgcag cggcttgccc tgcgcatcgc gctcgaacac cctcgaccgg tcgagtaccc
    10261 acacccagtg gccgtcggcg tgcagcatgc ggtaggtctg atcgaagtgt tcggcgcgac
    10321 cctccaggtg ctccagcttg gcctcgcgaa ctcgctccag gtcatcggga tggacacgcg
    10381 atcgccatgc ctgcgaatcg ggatagttct ggtgcggcag tccgagcatc gaattgatgc
    10441 gctcgctgac cagcgtggca ccgctcggca cgtcgaagtc ccacagcccc agatcggcac
    10501 cttccaacgc gagttcgagt cgcctggcac tctggtgctc ggattccttg cgctgcgcat
    10561 tcaggaaatc gatggcacgc tggcgacgct gcaccagcac cagggcgctc gatgtcacgg
    10621 ccgcgaacac ggagtacagc agcccgtaga ccaaggtctg cctccaccag gtcgcgaaca
    10681 tcgcatcgag gttgcgtccg atcgcgatca cgatgggctt gtcgacatac agatcggacg
    10741 gccggaccgt gcgcagcgcc accatccgct tgtctcccgt caccgggcac acaccggtca
    10801 gcaggctctc gagctgaccg gaagccacat gccgccggaa gaacgagccc ggtgtgtcga
    10861 cgttcattcc caccgctttg tcgctgcgcg gctcgaacat caggagccgt ccgtccgcgt
    10921 ggatgaccgc gccgcgcgaa tcggcggcgt acagcgtggg ccgaatggcg ctccggaagt
    10981 agtccggatc gagcatggat gtcaccaccc cggcgaaggc accgtctgcg gagcgcaccg
    11041 gcagggacag gttcatcgaa taggtgccca gcgagcccat gaagggcggg gacagcgaca
    11101 ggcgcttcgg atcggattga tcccgcgcca gctggaagta gtcgcgatca ctcaagtcgc
    11161 gtcccagcag atcggatcgg ttggctgcca ctgcgcgccc ggagcggtcc agcagcacca
    11221 gagaccccac accgggcatc gcattgccga gcgcctggag ccggctggac acacccgctt
    11281 cgacctcgcc cgccggacgc cgtgcgaggt cgtcgcgcag gtcccgcagc gcggcgccta
    11341 ctccggccag ctggcgcgac agggcctcgt cgacccccct ggcctgcacg accagcagat
    11401 gccgctgttc gtcatccacc tcgcggtagt cgctgtagcg cgcccagatc gcgaacagcc
    11461 ccacggcgat cagcaccgcg ccgagctgca gccactgctg gcgggcgcga gggcgacccg
    11521 gacccatgcc gctcgacgcc cccggggatg gggtgcttcc gggaatcatg attgcaagtt
    11581 accaccgacg ttgcatgctt gcccgagcgg acggcctgcc gacacggcgg cgcgcgcgga
    11641 cctgtcatcg cgtgcaacca ggcgccggca gcgcgccgtc gatcgcggcg gatgttcagg
    11701 cgatcgtgtt ggaagccagg tcgatcgcat cgcctgcatg gcggacgaac cagtaatagc
    11761 catctgcatc gcagcgcgcc atgccaccgc tgaggtacca gccgtcgcaa aaccgattgc
    11821 gatagcgctc ttcatcatgc agatagccac gcatcatcga gggccaaggg gcctccagcg
    11881 ccagttcgcc ttccacatcg ggccgggtga tcgtctcgac ccggccctgc gcatcgcggc
    11941 gcacgatggc tgcgcggatg cccggcagcg gcttgcccat cgagccgggc ttgatgtcca
    12001 tggccgcgta gttcgcgatc atgatcgcgc cgctctcggt ctgccaccag ttgtcatgga
    12061 acggcaggcc gaacacctcg atgccccaga tcacagcgtc cgcggtcagc ggttcgccga
    12121 cgctcgccat gaaacgcagc gccgaaagat cgtggctgcg cactgccacg gcatctgtat
    12181 ccatcaagtc gcggatttcc ggggcggcgg tgtaccagac gctgacccgc tcctgctcga
    12241 gcagcgcgca gcacgtggcc ggatcgaact cggcctcgac caccacgctg gtcacgccgg
    12301 cgaccagcgc cgcgatgatc ccgtagctgg taccggtgac gcagccgggg tctgccgtac
    12361 accagaagat gtcatcgtcg tggaagtcga gcgcgtaatg gccggtgact gcgtgagcca
    12421 gcacggcgcc gtgcacgtgg atcgtggtct gggttcggcc ctcggcggtg cgggtgaaat
    12481 gcagcagcgc caccgtctcg gtgtccgtgg cggccgtggt gaacctgtgc gaggcccctt
    12541 cgaccacgtc gccccagcga cgcacgccag ccccctcggc gaagcctgct ccgacaacga
    12601 tcacgtgctt cagcttcggc aaccgatgcc gcaagccggc gacccgagtc tggtagagct
    12661 ccgatgtggt caccagc
//
LOCUS       DAOORV010000103        12385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6310_length_12385_cov_6.996026, whole genome shotgun sequence.
ACCESSION   DAOORV010000103 DAOORV010000000
VERSION     DAOORV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6310_length_12385_cov_6.996026"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4419
                     /locus_tag="PKV56_13045"
     CDS             <1..4419
                     /locus_tag="PKV56_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOX68784.1"
                     /translation="DYRPGWVVRPDGRFDATYVATGSFDPQAGNYFNTNRYGDHDVVF
                     AGSGDDMVNAGGGDDYVDAGSENDIVRGFQGDDQLYGGAGSDDLLGDSYANIGLEDDK
                     FLGVSYRRYGLDPRVAGQEHGNDYIDGGADNDRIEGNGGADVLFGGGGNDNLFGDDSG
                     ASSAEFIPDAYSGNDFISGGDGDDFAQGGARDDDIQGGMGADALFGDNHFVDNVPAAG
                     MITAAGNDSIDGGWGDDLIWGEGGQDSLAGGSGNDQIFGDGSASDLRPDLHGVDVIDG
                     GAGNDTILGGGGDDILQGGEDNDWLAGENQLAAEETTTLTGDDVLLGGAGADTLLGGA
                     GADQLDGGAGNDLMLGGDGSDTYVFAVGAGNDVIINRDSDALGSGVDTIVLGAGITTE
                     NLIVQSSTGLSTSGSLVLGIAGATDSLLVSNYFSGNGTSSNAVEFIKFSSGLVWSVAD
                     VKLKVSGPVSTGVTKTGTEGSDTLSGGTGADKLFGLGGADVLTGADGDDYFVGGTGAD
                     TLNGGKGNDVYAFNLGDGADAIFDNSEYTASNDELRFGPGIAPNDLTFLRSGNDLIFK
                     FSNGADQITVKGWRASAYNQIEQVKFSDGSIWSAAEINTRSSQIIGTAGSDVLTGTSD
                     YLDAVLGLAGDDTLSASGGSDTLDGGAGNDVLNVGGLPYSNYVVTFLGGTGNDTLTGS
                     EYADTYIYNLGDGLDVITDHADYASSGDDELRFGSNIVSSDITPLRSGTDLVFQHRNG
                     SDGITVKNWFSDPFRREQIDRIVFSDVTWTADQVTGRAQPPLLGTAGNDALVGTPFAE
                     TLRGLAGNDTLTAAGSSGGDTLDGGDGNDVLKLLPETYSYVGATFIGGKGNDTIIGSV
                     YSDVYLFNVGDGADVVTDFNASYDDRDQLRFGPTIAASDITAIHAGQDLRLRHVNGLD
                     VITVNGWFSSDPVAYQIEALIFSDGTVWTADQVSTSAVSAPVLNGTPGADSVTGTASD
                     EVINGLAGNDTLDGGSGADTLLGGTGDDVYAVDNIADVVTELASEGRDIVRATLAWTL
                     GANLEDLVLAGSADINGIGNSLANALAGNSGSNRLDGAVGADSMTGGAGNDTYVVDNV
                     GDITVEVNGGGTDAVESSISWTLATEVEKLMLTGSAANNATGNANANTLIGNAGANRL
                     DGAAGADAMTGGAGNDTYVVDNAGDTTIEVSAGGTDAVESSTSWTLATAIEKLTLTGT
                     GGINGTGNSLANTLVGNSGANLLDGGAGNDTLSGGAGNDTYRVDSTLDVVTDNAGQGM
                     DTIQSTVSRTLGVNTENLTLIGTAAINGTGNALANLLVGNAGANTLTGNAGNDSLEGA
                     AGADALAGGDGNDRYVGGLGADSLVDTSTTSSDVYVWGRGEGADVLTDSGGTDRLDLL
                     AGVTEGQIWLRQVGSNLELSVIGTTDRLTMNGWYANPANRVESFRLSDGQALLASQVQ
                     QLVDAMAAFAPPAAGQTTLPANYQTALNPVIAPSWA"
     gene            complement(4521..6338)
                     /locus_tag="PKV56_13050"
     CDS             complement(4521..6338)
                     /locus_tag="PKV56_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOX68785.1"
                     /translation="MTRRRLRTFGLFAASLCAIMLCRPAGAAVTASVDRTQLAPGDTI
                     ELTLQSDRGGSGQPDLKALEKDFEILRRANSNSLQIVNGSASSQRQVRLTLSPRRKGR
                     IEVPRLEWEGEQSAVIELVVAAGNGSAGQGAQAGSVADVFFTTTLDSLQPHVQSAVSL
                     QLRLYSDKKLYQASVDFPGNTDLLVRRIGQDESSQETRNGRTYQVITRSFVLVPQRSG
                     EIQLEGPVLNAQVADGTRSLDPFMERVFGQLQIEGGASGTRALRLRGDPVRLTAKPRP
                     PGMGSGDWLPAQQLTIDEAWQPAGGTIAVGVPVTRRLRVSAVGLSASQLPDLGALMAL
                     PAGLTSHPGAPKLTEEVTDGHIVGHREQEIVVLADRAGHFELPAMQVAWWDLAAAQRR
                     ETMLPALALDVTPGPIATPAAPAPTAGTTAATGEAQNGPPAAAPSASRADVPASSGPP
                     STKWIGVSITLALLWLATLGAWAWTHRQRRLSRALGASSPGKQAAAQVSAAAAMKELR
                     HACHSQSARSARDALLSWARATWPQSAPPGLNALARRLNQADVTSLLRELDRACVEGS
                     AWNGEALARSLGALERQNGPEITRSVLPGLYDEQENRRV"
     gene            complement(6335..7225)
                     /locus_tag="PKV56_13055"
     CDS             complement(6335..7225)
                     /locus_tag="PKV56_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX68786.1"
                     /translation="MNVASRHLWLTPLLLACCTTAHAEGGWSSLWRTPDQRGEAQLRD
                     GDAAAAARSYADPRRRAYAQMKSGDFRAAAETYATLDDADAQYNRGNALARTGDLQQA
                     LGAYDAALAKNPADRDARRNRDLVAQALQQPPARSGQGPKPNALGGGPGAQPPAQAAS
                     GPPGEAQTARGSAATQSPGPSGQPIDGAADRSGPSDAREGGSSVVSPSGTATANGQTE
                     GPGTDQARADDAAQAQRDVFGAVKRPSGARDPRDARPRQRSEQQLAEDQWLRRLPDDP
                     AGLLRRKFLIQHLIRQGTPP"
     gene            complement(7222..8238)
                     /locus_tag="PKV56_13060"
     CDS             complement(7222..8238)
                     /locus_tag="PKV56_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX68787.1"
                     /translation="MEMIADLQAALGRFHFLRPAWLLAVPLLLFLAGWLARRSASAGA
                     WSSLIDAPLLAALRLAEPSSRRTTPWPWLALAWTLAAMALAGPTWQREPATAYSAPDG
                     WVVVLDLSASMAAQDVAPDRATRARYAINDLLRAAQGARIGLVVFGAEPFLVTPLTDD
                     VATVRGLSNTLAPDLLPGAGDRLAPALVMAQQLLERTATTGGHVVVLSDGFEDPVPAV
                     AAAEMLRARKTTLDVVGIGTAGGALVPLPKGGFLQDAQGRIEMARLDSDLLQKLALAG
                     GGRAFGLDQVPALISGLQSYATGGGRATEVDDLKAARWRDAGGWLLPAVLLLVAGLAR
                     RGWW"
     gene            complement(8229..9197)
                     /locus_tag="PKV56_13065"
     CDS             complement(8229..9197)
                     /locus_tag="PKV56_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX68788.1"
                     /translation="MIHFEWPWMLLAAPLPWAIARWLPPARPHDAALFLPFAAQIAAR
                     AAPVAPRAARWRVYLFALVWLLLIAASVRPQWLGEPQPVATTGRRLLLAIDVSGSMAA
                     RDMAGRASRLQVVKDLAGEFIQRRHGDQVGLILFGTRPYLQAPWSTDLDTVNQFLQEA
                     TIGMAGPATALGDAIGLALKLLHNDREADAARQQVGASGETVLVLLTDGSSNAGEMPP
                     VPAARLAAASRLRVYTIGVGGGGDEDSDLDEATLKTIAAETGGAYFQATDAAALQEAY
                     ARMDRLEPAAANDRWVQPSDEWFVWPLALALLLSVPAAALREPRWK"
     gene            complement(9194..9700)
                     /locus_tag="PKV56_13070"
     CDS             complement(9194..9700)
                     /locus_tag="PKV56_13070"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HOX68789.1"
                     /translation="MTPSWLSQLAPDRAPPPLAWWHLAPGWWGLAALLASALVLALWW
                     RRRPRRPSFRHWQRAALHELAQLQATSSREVDSDATIARGLQQLLRRYAVVRHGRERV
                     AALNGDAWIAFVVDHGGTAWAGETGRRLLRCAYGGDVPSRAGTAEVRTDDRERWFAGA
                     RAFVKARG"
     gene            complement(9697..10593)
                     /locus_tag="PKV56_13075"
     CDS             complement(9697..10593)
                     /locus_tag="PKV56_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX68790.1"
                     /translation="MGLLPDLDELVVLRGAARDLEMRIRQPAASQLQGAHRSAHRGRG
                     LEFDEVRVYAPGDDARTIDWRVTARRGRPHTKLFREERERQVWLLADLHPGLYFGSRR
                     QLKSAQLLRAAAWLAWVAVDAGDRLGGIITHGSSAPEALPPRSRDIGALALLQALVQN
                     QPRAPGPMASGALGKALTTLRPLLRPGSSVVVLSDFSDFDDAGESALVALSMRAECRV
                     VWLTDPLERQGLPAGAYRVGVPGRLWSLDGAASRGDWSAAWAERERRVDSLCRSRRLP
                     LLRLDTGDDLAQRMPAFISESR"
     gene            complement(10636..11637)
                     /locus_tag="PKV56_13080"
     CDS             complement(10636..11637)
                     /locus_tag="PKV56_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX68791.1"
                     /translation="MDSLMTPPNSPGNTPQDAFARLRHYLEQQVLGQPVLVERLLVAL
                     LADGHLLVEGPPGLAKTRAVKALAHAVECDFQRVQFTPDMLPADLTGSDIYRPEEGSF
                     RFQRGPLFHNLILADEINRAPAKVQSALLEAMAERQISVGATTYHLPALFMVMATQNP
                     IEQEGTYPLPEAQLDRFMLHTRVDYPDRATTRLIMELARREAVSAVELPPPARRLSQA
                     AVFAARRQVLDVSLADSVADYIAALVDATRRPGPYSELLAGWLRWGASPRAAIAIERG
                     ARALAWLDGRDYVSPEDVQAIAPDALRHRLLLDYTAQAARVTAQDCVDELLRQVPAP"
     gene            11872..>12385
                     /locus_tag="PKV56_13085"
     CDS             11872..>12385
                     /locus_tag="PKV56_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX68792.1"
                     /translation="MNEQTAAVVASGHIPSRIAKTAGGFRVRPNLVDYAAVQAAFSWE
                     RARESLAGLPGGGLNIAYEAVDHHLGTAHADRTAIRWIGKAGARRELSYRDLAHATNR
                     FANALHGLGTQPGDRVCVLMGRLPEFHVAVLGALKAGCVAAPLARESVTEQGALCCAL
                     AEAHVLVTTSE"
BASE COUNT         2024 a         4067 c         4310 g         1984 t
ORIGIN      
        1 gattacaggc ccggttgggt cgtgcgcccg gatggcaggt tcgacgccac ctacgttgct
       61 acgggctcct tcgacccgca ggccggcaat tacttcaata ccaaccgcta cggtgatcac
      121 gatgttgtct tcgcgggttc cggcgacgac atggtcaacg ctggtggggg tgacgactac
      181 gtcgacgccg gcagcgagaa cgacatcgtc cggggctttc agggcgatga ccagttgtac
      241 ggaggggccg gcagtgacga cttgctgggc gacagctacg cgaacatcgg actggaggac
      301 gacaagttcc tcggggtgag ctatcgccgg tacgggctgg accctcgcgt cgccgggcag
      361 gagcatggca atgactacat cgatggcggt gccgacaacg accggatcga aggcaatggc
      421 ggtgccgatg tgctgttcgg tggcggcggc aatgacaacc tctttggcga tgacagtggg
      481 gccagcagtg ccgaattcat tcccgatgcc tattccggga acgatttcat ctccggcgga
      541 gatggtgatg actttgccca gggtggcgcc cgcgatgacg atatccaggg tgggatggga
      601 gcggacgcgc tattcggtga caaccacttt gttgacaacg ttccagcggc cgggatgatc
      661 acagcggctg gcaatgacag catcgacggg ggctggggtg acgacttgat ctggggcgag
      721 ggcgggcagg attcgctcgc gggtggcagt ggcaacgacc agatattcgg tgatgggtct
      781 gccagcgatc tccggcctga cttgcacggc gtcgacgtca tcgatggggg tgccggcaat
      841 gacacgatcc tcggcggcgg tggcgatgac atcctgcagg gcggtgagga caacgattgg
      901 ctggcgggag agaaccagct ggctgcagag gagaccacca cgctcaccgg cgatgacgtc
      961 ctgctgggcg gagccggggc agacacgctg ctcggtggtg cgggcgcaga ccaactggat
     1021 ggcggcgccg gcaacgatct gatgctgggc ggcgatggtt ccgacaccta cgtgtttgct
     1081 gtcggcgcgg gcaatgacgt gatcatcaat cgcgacagcg atgccctggg cagcggggtg
     1141 gacaccatcg tgctcggggc agggatcacc acggagaacc tgattgtcca gagctcgacc
     1201 ggcttgagca cgtcgggaag cctggtactg ggaattgcag gcgccaccga ctccttgctg
     1261 gtcagcaact acttttccgg taatggaacg tccagcaacg cggtggagtt catcaagttc
     1321 agcagtgggc tggtctggag cgtggccgat gtgaagttga aggtcagcgg gcccgtatcc
     1381 acaggcgtca cgaagaccgg cactgaaggc agcgacaccc tgtctggtgg caccggcgcg
     1441 gacaagcttt tcgggctggg gggtgcagat gtgctgacag gggccgatgg ggacgactac
     1501 ttcgtgggtg ggacgggcgc ggacaccctg aacggtggca agggcaacga tgtctacgct
     1561 ttcaatcttg gtgatggtgc agatgcgatc ttcgacaaca gcgagtacac cgccagcaac
     1621 gacgagttgc gattcggtcc cggcatcgca ccgaatgacc tcaccttctt gaggtccggc
     1681 aacgacctga tcttcaagtt ttccaatggg gctgatcaga tcaccgtcaa gggttggcgc
     1741 gccagcgctt acaaccagat cgagcaagtc aagttcagcg atggatccat ctggagcgcc
     1801 gccgagatca acacccgttc gagtcagatc atcgggaccg ccggttccga tgtgttgacg
     1861 ggcacgtccg actacttgga cgcagtgctc ggactggctg gcgatgacac cctcagtgcc
     1921 tccggaggaa gcgacacgct cgacggtggc gcaggcaacg atgtgctcaa tgtcggcggt
     1981 ttgccctact ccaattacgt ggtgactttt ctgggtggca cgggcaacga caccctgacc
     2041 ggttcggaat acgccgacac ctacatctac aacctgggtg atggcctgga cgtgatcact
     2101 gaccatgcgg actacgcgag cagcggcgac gacgaactgc gttttggttc aaacatcgtc
     2161 tcgtccgaca tcacgccgct tcgctccggc accgacctgg tctttcagca tcgaaatggc
     2221 agcgacggga tcacggtcaa gaactggttt tccgacccct tccgtcgaga gcagatcgac
     2281 aggatcgtct tcagtgacgt cacgtggacg gcggaccagg tcactggccg ggcccagccg
     2341 ccgctgctgg gcaccgctgg caacgacgcg ctggtcggca caccctttgc cgagacgctg
     2401 cgcggattgg ctggcaacga cacgctgaca gccgcaggca gcagtggtgg cgacacgctg
     2461 gacggcggcg acggcaacga tgtgctgaag ttgctgcccg aaacctacag ctatgtcggc
     2521 gccaccttca tcggcggcaa gggcaacgac accatcatcg ggtcggtcta cagcgacgtc
     2581 tacctgttca acgtgggaga tggtgccgat gtcgtgaccg acttcaacgc cagctacgac
     2641 gatcgtgatc agttgcgctt cggcccgacc attgcagctt cggatatcac agccattcat
     2701 gccgggcagg atctgaggct ccggcatgtc aacggactcg atgtcatcac ggtcaacggg
     2761 tggttctcga gcgatcccgt cgcctatcag atcgaagcgc tgatattcag cgatgggacc
     2821 gtctggacgg ccgatcaggt cagcacgagc gcggtcagtg cccctgttct caacggcacg
     2881 ccaggcgccg attctgtgac gggtaccgcg agtgacgagg tgatcaacgg cctggccggc
     2941 aatgacacgc tcgacggagg atcgggcgcc gataccttgc tgggcggcac gggcgacgac
     3001 gtctacgccg tggacaacat cgccgacgtc gtcaccgaac tggccagcga aggacgtgac
     3061 atcgtgcggg ccacgctcgc gtggacgctg ggggcgaacc tggaagatct cgtgcttgcc
     3121 ggatcggcag acatcaacgg tatcggcaac tccctggcaa acgcactcgc aggcaactcg
     3181 ggcagcaatc gactcgatgg ggccgtcggt gccgacagca tgacgggcgg cgctggcaat
     3241 gacacctatg tcgtcgataa cgtgggcgac atcacggtcg aggtcaacgg cggaggaact
     3301 gatgcggtcg aatcgtctat cagctggacc ctggcgaccg aagtcgagaa gctcatgctc
     3361 acaggctcgg ctgccaacaa tgcgacgggc aatgcaaacg ccaacacact gatcggcaac
     3421 gcaggcgcca accggctcga cggtgcagcg ggtgctgatg ccatgaccgg aggtgccggc
     3481 aacgacacct acgtggtcga taacgcaggc gacaccacca tcgaggtttc tgcgggtggc
     3541 accgatgctg tcgagtcgtc gaccagctgg acgcttgcca ccgcgatcga gaagctcacc
     3601 ctgaccggga ccggcggcat caacggcacc ggcaacagcc tggccaacac cctggtcggc
     3661 aacagcggag ccaacctcct tgacggaggc gccggaaacg acaccctgag tggcggtgca
     3721 ggcaatgaca cctatcgggt cgacagcacg ctcgatgtcg ttaccgacaa cgccggtcag
     3781 ggcatggaca cgattcagtc gaccgtctcg aggacgctgg gcgtcaacac cgagaacctg
     3841 acgttgatcg gtacggcagc catcaacggg acgggcaacg cgctggccaa tctgctggtt
     3901 ggaaacgcgg gtgcgaacac gctcaccggc aacgccggca acgacagtct cgagggcgct
     3961 gcaggagccg atgcattggc gggcggcgat ggcaacgatc gctatgtcgg aggtttgggg
     4021 gcggacagtc tcgtcgacac gtcgaccact tccagcgatg tctatgtctg ggggcgtgga
     4081 gagggggccg acgttctgac agactccggc ggcacggatc gcctggatct gcttgcaggg
     4141 gtgaccgagg gccagatctg gctgcgtcag gtcggcagca acctggagct ttccgtcatc
     4201 ggcacgaccg accgcctgac gatgaacggt tggtatgcca acccggccaa ccgcgtcgag
     4261 agtttccggt tgtctgatgg acaggccttg ctggcctctc aggttcagca actcgtcgac
     4321 gccatggcgg cgttcgcacc gccggcggct gggcagacga cattgcctgc gaactatcag
     4381 actgcgctga acccggtgat agcgccgagc tgggcctgag ctatccgagg ccccgggggc
     4441 gcagcctctg cagcggcagg caggcgtttg ggccgctcac cggaagctgc ggcgctcgga
     4501 tcgacgcagc ctctgcacgc tcagacgcgt cggttttcct gctcgtcata caggccgggc
     4561 agcaccgatc gggtgatctc cgggccgttc tgtcgttcca gcgcgcccag actgcgggcc
     4621 agcgcttcgc cgttccaggc gctgccctcc acgcaggcgc ggtccagctc gcgcagcagc
     4681 gaggtgacgt cggcctggtt gagccggcgg gccagcgcgt tgaggccggg cggtgcggat
     4741 tggggccagg tggctcgtgc ccaagacagc agtgcgtcgc gtgccgatcg cgcagactgg
     4801 ctatggcagg cgtgccgcaa ctccttcatc gctgcggccg cgctcacttg ggctgctgcc
     4861 tgcttcccgg gtgaggatgc gccgagggcg cgcgatagcc gccgctgccg atgggtccag
     4921 gcccaggctc cgagggttgc gagccacagc agagccaggg tgatgctgac ccctatccat
     4981 ttggttgaag gcggtccgct gcttgccggc acgtcggcgc gggatgcaga gggtgctgcg
     5041 gccggggggc cgttctgggc ttcgccggtc gcagcggtcg tgcctgcggt gggggcgggt
     5101 gcggctggcg tggcgatagg gccgggcgtc acgtccagcg cgagcgcggg caacatcgtc
     5161 tcgcgccgtt gcgccgccgc caaatcccac caggccacct gcatggcggg cagctcgaag
     5221 tggcctgcgc ggtctgccag cacgacgatc tcctgctcac ggtgtccgac gatgtggcca
     5281 tccgtcacct cttcggtgag cttgggtgcg ccggggtgcg aagtaagacc cgccggcagg
     5341 gccatcaggg cgcccagatc gggcagctgg cttgcactga gcccgacagc ggacacgcgc
     5401 agacggcgcg tcaccggcac acccacggcg atcgtgccgc ctgccggctg ccaggcttcg
     5461 tcgatcgtca gctgttgtgc aggcagccag tcgccgctgc ccatgcccgg tggccgaggc
     5521 ttcgcggtca ggcggaccgg gtcgcctcgc agccgcaggg cccgcgtgcc gctcgcgccg
     5581 ccctcgatct gcaactggcc gaacacgcgc tccatgaacg ggtcgagcga tcgtgtgccg
     5641 tcggccacct gcgcgttgag taccggccct tcgagctgga tctcgccgct gcgctgcggc
     5701 accagcacga agctgcgcgt gatcacctga taggtgcggc cgttgcgggt ttcctggctt
     5761 gattcgtcct gcccgatgcg ccgcaccagc aggtcggtgt tgccgggaaa gtcgacgctg
     5821 gcctggtaca gcttcttgtc gctgtaaaga cggagctgca ggctcaccgc cgactgaacg
     5881 tgcggctgca gggagtcgag cgtcgtcgtg aagaacacat ccgcgaccga gccggcttgc
     5941 gctccctggc cggcggagcc gttgcccgcc gcgacgacca actcgatgac ggccgattgc
     6001 tcgccttccc attcgagcct cggcacctcg atgcggcctt tgcggcgcgg cgacagggtc
     6061 agcctcacct gccgctgcga cgacgccgat ccgttgacga tctgcaggct gttgctgttt
     6121 gcgcggcgca ggatctcgaa gtccttttcc agcgccttga gatcgggctg gccgctgccg
     6181 ccgcgatcgc tttgcagcgt cagctcgatc gtgtcgccgg gcgcgagttg cgtgcggtcg
     6241 accgacgcgg tgacggccgc cccggcgggt ctgcacagca tgatggcgca caggcttgcc
     6301 gcgaacaggc cgaaggtgcg caggcgacgt cgggtcatgg cggggtcccc tgtcggatca
     6361 ggtgctgaat caggaacttg cggcgcagca atcctgccgg atcgtccggc aggcggcgca
     6421 gccactggtc ttcggccagt tgctgctcgg agcgctgccg gggccgggcg tcgcgcggat
     6481 cgcgagcccc ggacgggcgc ttgaccgcac cgaacacatc acgctgcgcc tgtgccgcgt
     6541 cgtcggcccg ggcctgatcg gttccgggtc cttcggtctg cccgtttgca gtcgcagtgc
     6601 ccgacggact gaccaccgaa gagccacctt cccgggcgtc ggatggcccg gaacgatcgg
     6661 ctgctccatc gatcggctgc cccgaaggcc cggggctctg ggtggccgcc gatccgcgcg
     6721 cggtctgcgc ttcaccaggc gggccggaag ccgcttgagc gggcggctgt gccccgggtc
     6781 cgccgcccag cgcattgggc ttcggaccct gcccggatcg ggcgggcggt tgctgaaggg
     6841 cctgcgccac cagatcgcga tttcgtcgcg cgtcgcggtc tgccgggttc ttcgccagcg
     6901 cggcgtcgta ggcccccagg gcttgttgca gatcgccggt gcgcgcaagc gcgttgccgc
     6961 ggttgtattg cgcatcggcg tcgtccagcg tcgcgtaggt ttcggcggct gcgcggaagt
     7021 cgcccgactt catctgtgcg taggccctgc gccgcgggtc ggcatatgac cgcgctgccg
     7081 cggcggcgtc gccgtctcgc agctgggcct cgccgcgctg atccggcgtg cgccacagcg
     7141 acgaccagcc gccctcggcg tgtgccgtcg tgcagcaggc cagcagcagc ggggtcagcc
     7201 agaggtgtct cgaggcgacg ttcaccacca gcctcgtcga gccagtcccg ccaccagcag
     7261 cagaaccgct ggcagcaacc acccgccggc gtctcgccag cgcgcggcct tcaaatcgtc
     7321 cacctcggtg gcacggccgc cgccggttgc atacgactgc agcccggaga tcagcgcggg
     7381 gacctgatca agcccgaatg cgcgcccgcc tcccgccagg gccagcttct gcagcaggtc
     7441 gctgtcgagc cgtgccatct cgatgcggcc ctgcgcgtcc tgcaggaacc cgcctttcgg
     7501 caagggcacc agcgcaccac ctgcggtgcc gatgccgacc acatcgagcg tcgtcttgcg
     7561 cgcccgcagc atctcggctg cggcgacagc gggcaccgga tcctcgaagc cgtcgctgag
     7621 aacgaccacg tgaccgccgg tcgttgcagt gcgctccagc agttgctgcg ccatcacgag
     7681 cgccggggcc agcctgtcgc cggcgccggg aagcagatcg ggcgcgagcg tgttgctcag
     7741 gccgcgcacg gtggccacgt cgtccgtgag cggggtcacc aggaagggct cggccccgaa
     7801 caccaccaga ccaatgcgcg cgccctgggc cgcgcgcaac agatcgttga tcgcgtagcg
     7861 tgcgcgcgta gcgcggtccg gcgccacgtc ctgggccgcc atcgacgcag acaggtcgag
     7921 caccaccacc catccgtcgg gcgccgaata cgccgtggcc ggctcgcgct gccaggtcgg
     7981 accggccagt gccatcgcgg ccagagtcca ggccagcgcc agccacggcc agggtgtcgt
     8041 tctgcgactt gacggctcgg ccaggcgcag cgcggccagc agcggtgcat cgatcagcga
     8101 cgaccacgct cctgcggacg cgctgcgccg tgccagccac cctgcgagga acagcagcag
     8161 cggcaccgcc agcaaccacg ccgggcgcag gaaatggaag cggccgaggg cggcctgcag
     8221 gtcagcgatc atttccatcg cggctcccgc agggctgcgg ccggcacgct cagcagcagg
     8281 gccagcgcaa gtggccagac gaaccattca tcgctcggct gcacccaccg atcgttggct
     8341 gcggccggct ccagcctgtc catgcgcgca taggcttcct gcagagctgc ggcatcggtg
     8401 gcctggaagt acgcgccgcc ggtttccgcc gcgatggtct tcagcgtggc ttcgtcgaga
     8461 tcgctgtctt cgtcgccacc gccgccgact ccgatcgtgt agacgcgcag ccgcgacgca
     8521 gccgccagcc tggccgctgg cacaggtggc atttcgcctg cgttgctgct gccgtcggtg
     8581 agcagcacca gcaccgtctc gccactggcg ccgacctgtt gccgggctgc gtcggcctca
     8641 cgatcgttat gcagcagctt gagggccagt ccaatggcgt cgcccagcgc ggtggcgggt
     8701 ccggccatgc cgatcgtcgc ttcctgcagg aactggttga ccgtgtccag gtcggtactc
     8761 catggcgcct gcaggtaggg gcgggtgccg aagaggatca ggcccacctg gtctccatgc
     8821 cgccgctgta tgaactctcc ggccagatcc ttgaccactt ggaggcggct ggcgcgtccc
     8881 gccatgtcgc gcgcggccat cgagcccgac acgtcgatcg ccagcagcag gcgacgcccg
     8941 gtggtggcca ccggttgcgg ctcgccgagc cactgcggac gcacggatgc ggcgatcagc
     9001 agcagccaga ccagcgcgaa cagatacacc cgccagcgtg ctgcccgcgg cgcgaccggc
     9061 gcagcgcgtg cggcaatctg cgccgcaaag ggcaggaaca gggctgcgtc atgcgggcgg
     9121 gcgggcggca accagcgggc gatcgcccac ggcagtggcg cggccagcaa catccacggc
     9181 cattcgaagt ggatcatccg cgggccttga cgaaggcgcg agcgccggcg aaccaccgct
     9241 cgcgatcgtc ggtgcggacc tctgccgttc cagcgcggga cggcacatcg ccgccatagg
     9301 cacagcgcag cagccttcgg cccgtctcgc ccgcccaggc ggtgccgcca tggtcgacca
     9361 cgaaggcgat ccaggcgtcg ccgttcaacg ccgccacgcg ttcccgcccg tggcgcacca
     9421 ccgcgtatcg gcgcagcagc tgctgcaagc cgcgcgcgat cgtggcgtcg gaatcgacct
     9481 cccggctgga tgttgcctgc agctgcgcaa gctcgtgcaa ggcggcgcgc tgccagtgtc
     9541 gaaaggaagg ccggcgcggc cgccgacgcc accagagcgc aagcaccagt gcgctagcaa
     9601 gcagggctgc caggccccac cagccaggcg ccagatgcca ccaggcgagc ggtggtggcg
     9661 ctcggtccgg ggccaattgg gacagccagg acggggtcat ctcgactcgc tgatgaacgc
     9721 gggcatccgc tgcgccagat cgtcgccggt gtcgagccga agcagcggca ggcgacgact
     9781 ccggcagaga gaatccacgc gccgctcgcg ttctgcccag gcggccgacc agtcgcctcg
     9841 actggcggcg ccgtcgagcg accagagtcg ccccggcacc ccgaccctgt aggcacctgc
     9901 cggaaggccc tgtcgctcca gtggatcggt gagccagacc acacggcatt cggcgcgcat
     9961 cgagagggcc accagtgccg actcgcctgc atcgtcgaag tcgctgaaat cgctcagcac
    10021 gaccacggaa ctgccggggc gcagcagcgg acgcagcgtg gtcagtgcct tgcccagggc
    10081 gcctgatgcc atcggccccg gagcgcgcgg ctggttctgc accagcgcct gcaacagcgc
    10141 aagcgcaccg atgtcgcgcg atcggggtgg cagcgcttcg ggcgcggacg agccgtgcgt
    10201 gatgatgccg ccaagccggt cgccagcgtc caccgcgacc caggcgagcc aggctgcggc
    10261 tctgagcagt tgggccgact tgagctgccg gcggctgccg aagtagagac ccggatgcag
    10321 gtcggccagc agccacacct ggcgctcgcg ctcttcgcgg aagagcttcg tatgggggcg
    10381 gccgcgccgc gccgtgacgc gccagtcgat ggtgcgcgcg tcgtcgcccg gggcatagac
    10441 gcgcacctca tcgaactcga gccctcgacc gcggtgggcg ctgcggtgag cgccctgaag
    10501 ctgcgatgcc gcgggctggc ggatgcgcat ttccaggtcg cgcgctgcgc cgcgcagcac
    10561 gaccagttcg tcgaggtcag gcagcaggcc catgccattc cgactgtctg cccgtgtgct
    10621 gtcgattcga acccatcaag gtgccggcac ctggcgaagc agttcatcga cgcaatcctg
    10681 cgccgtgacc cgcgcggcct gtgctgtgta gtcgagcagc aggcggtggc gcagtgcgtc
    10741 gggcgcgatg gcctgcacgt cctccggact gacgtagtcg cgcccgtcga gccaggccag
    10801 cgcgcgcgca ccgcgttcga tcgcgatcgc ggcgcgcggg ctggctcccc agcgcagcca
    10861 gccggccagc aattcgctgt aggggcccgg ccggcgcgtc gcgtcgacca gagccgcgat
    10921 gtagtcagcc accgagtcgg cgagggatac gtccagcacc tggcgccgcg cggcgaacac
    10981 ggctgcctgg gacagtcgcc gggccggcgg cggcagttcc acggccgaca ccgcttcgcg
    11041 ccgcgcgagt tccatgatca ggcgggtggt ggcgcggtct gggtagtcga cgcgtgtgtg
    11101 gagcatgaag cggtcgagtt gcgcctcggg caacgggtag gtgccttcct gctcgatcgg
    11161 gttctgggtc gccatgacca tgaacagcgc gggcaggtga taggtcgtgg cgccgacgct
    11221 gatctggcgt tcggccatcg cctcgagcag cgccgactgc accttggccg gtgcacggtt
    11281 gatctcgtcg gccaggatca ggttgtggaa cagcggtccg cgctggaagc ggaagctgcc
    11341 ttcctcaggc cggtagatgt cggagccggt cagatcggcc ggcagcatgt cgggcgtgaa
    11401 ctgcacgcgc tggaagtcgc actccacggc atgcgccagt gccttgaccg cgcgggtctt
    11461 ggccaaaccg ggcgggcctt cgaccaacaa atggccgtcc gccagcaacg cgacgagcag
    11521 ccgttcgacg agcaccggtt ggccgaggac ctgctgctcg aggtagtggc gcagtcttgc
    11581 gaacgcgtcc tgcggtgtgt ttccaggcga attcgggggc gtcatcaagg agtccatcgg
    11641 tgggattttc catctgcggt gcgcatgcac cctttgaaca ggcgcctgtg cgcagcccgg
    11701 tgtcgaacga atgtacgtcg gacaagcagt gccggatgca cctgcccgtg ggggcgtagc
    11761 ttgatgtcga tcaacgccgc ggttgcatgc gcggccatca tggccgcgtc gcgttgcagg
    11821 ttcccgtcgg gtgttcgccc cgggccggct gtcgcggcga caggtgatcg aatgaatgag
    11881 cagacagcgg cagtggttgc gagcgggcac atcccgtcgc gcattgccaa gacagcaggt
    11941 ggatttcgcg tccggccaaa cctggtcgat tacgcggccg ttcaagctgc attcagctgg
    12001 gagcgcgcgc gcgagagcct ggccggactt cccggcggcg ggctcaacat cgcctacgag
    12061 gctgtcgacc atcacctggg caccgcgcat gccgatcgca ccgcgatccg ctggatcggc
    12121 aaggccggtg ctcggcgtga gctgagctat cgggacctcg ctcacgcgac caaccgattt
    12181 gcaaacgcgc tgcacgggct cggcacgcag ccgggcgatc gggtctgcgt gctgatgggg
    12241 cgcctgccgg agtttcacgt cgcggtgctg ggggcactga aggcgggttg cgtcgcggct
    12301 ccactggctc gcgaatcggt cacagagcag ggtgccttgt gctgcgcgct ggcggaagct
    12361 cacgtgctgg tgaccacatc ggagc
//
LOCUS       DAOORV010000104        12174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6460_length_12174_cov_7.090849, whole genome shotgun sequence.
ACCESSION   DAOORV010000104 DAOORV010000000
VERSION     DAOORV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6460_length_12174_cov_7.090849"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKV56_13090"
     CDS             <1..628
                     /locus_tag="PKV56_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOX68793.1"
                     /translation="GPNTKFLLAQPFLADTARALESCGAQRLAAPFPLGVEGTTSWLQ
                     AAATAFGIAAATFDRVTAPGRDRAATALARHRVQLAGKRVFFFPDSQLEVPIARFLSR
                     ELGMQLIEVGTPYLHRQHLAEELDLLPAGTFLSEGQHVDNQLDRCRQAQPDIVVCGLG
                     LANPLEAEGMTTKWAIELVFTPIHGYEQAADLAELFARPLVRRMKLVA"
     gene            666..2297
                     /gene="bchB"
                     /locus_tag="PKV56_13095"
     CDS             666..2297
                     /gene="bchB"
                     /locus_tag="PKV56_13095"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit B"
                     /protein_id="HOX68794.1"
                     /translation="MQLTLWTYEGPPHVGAMRIATALKDVHYVLHAPQGDTYADLLFT
                     MIERLPKRPPVTYTTFQARDLGGDTAELFKTAAREAYERFKPKAMMVGASCTAELIQD
                     DPGGLCKALNLPVPVVALELPSYQRKENWGASETFYQMVRALAGPNAPAPGTAKPGRV
                     AGARPRCNILGPAALGFRHRDDLREITSLLAQLGIEVNVVAPLGATADDLSRLGDADF
                     NVVLYPEIASLTASWLTRIFGQPATKTIPIGAGATREFIKEVAQLADIDPSAMLANSD
                     SRSPWYAKSVDSTYLTNKRVFVFGDATHAVAAARVASQELGFKVVGIGTYSREFAREV
                     REAAKLYDVDALISDDYLEIEARISELRPELVLGTQMERHIAKRLGLPCAVISAPVHV
                     QDFPARYSPQMGFEGANVLFDTWVHPLMMGLEEHLIGMFRGDAEFHEDAGPSHLGGHA
                     APRAHASGPGIVESETLGAELVAGAEKQIDQNAESPAAAIPASWAPDAEKELRKIPFF
                     VRGKARRNTERYAQEHSVPVITVETLYDAKAHFSS"
     gene            2269..6084
                     /locus_tag="PKV56_13100"
     CDS             2269..6084
                     /locus_tag="PKV56_13100"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit H"
                     /protein_id="HOX68795.1"
                     /translation="MTQKLISAAEVRSKIHHTPIRVVVVTMDTHLASSTQRARATLAR
                     ELPGLQLSMHAASEYAGNSAALERCRADIAQADIVVAGMLFLEDHFLPILPDLLARRD
                     HCDAMICMASAGEIVKLTRLGNFDMTKPSSGPMALLKKLRGNKEKSAPGGAAQMKMLR
                     RIPQMLRFIPGTAQDVRAYFLTLQYWLGGSDENMLNLVRHVVDRGADGPRRVLRGLVK
                     VSVPVEYPEVGVYHPRMPGRLSADASALPKPPCKEIRGTVGVLVLRSYLLSNNAGHYD
                     GVISALESRGLRVIPAFSAGLDARPAIDHFFRKDAEILVDAVVSLTGFSLVGGPAYND
                     AKAAEEVLAQLDVPYVAAHPVEFQTLDQWGASDRGLLPVESTIMVAIPELDGSSSPIV
                     FGGRPGAAGVTCTGCSHACNFQESDNAKDMHACPERAAMLAARVLKLVALRRSERAQR
                     KVAIVIFNFPPNAGNIGSAAYLSVFESLFHTLTSMKAQGYKVDLPESIDALRDSVLKG
                     NAAQHGADANVHTLISAEDHVRRERWLQEIEGQWGPAPGRQLSNGNSILVLGKQFGNV
                     LVGVQPAFGYEGDPMRLLFEKGLAPTHAFSAFYRYLREDFKAHAVLHFGTHGALEFMP
                     GKQSGLGGMCWPDRLIDDLPNIYLYASNNPSEGAIARRRSGATLISYLTPPVAQAGLY
                     RGLNDLRASIERWRTLEPRSADRAELAILVQSQAAELDLASAEPPWDAAEGGDADTRV
                     ARLTEQMLELEYTLIPHGLHVTGRPPSVAERVDMLLAMADASHGVQLERASIEALVGG
                     GSAERALELGHLPRDEASMEMMRGLAKADAMLAVDTEVEAILHALDGRFIRPAPGGDV
                     LRTPAVLPTGRNIHGFDPFRIPSTFAVKDGFKQAQLLIDRHLADGHGLPESIAMVLWG
                     SDNLKNEGAPIAQALALMGALPRFDSYGRIAGATLIPLSELGRPRIDVVITLSGIFRD
                     LLPLQIKLLAEAAYLCASAADEPIEMNWIRKHALAYQQEHGCTLETAALRVYGNAEGA
                     YGSNVNNLVESGKWSDEGEIAETYTRRKGFAYGRTGRAVQQTALLKSALADVQLTYQN
                     LDSVELGVTTVDNYFDTLGGITQAVKVAKGGRSSDVPPVYIGDQTRGDGAVRSLREQV
                     ALETRTRMLNPKWYEGMLEHGYEGVRQIEVHVTNTMGWSATTGQVQPWVYQQLSETFM
                     LDPEMRERLAKLNPTASVRVANRLLEASARNYWQPDAATLDALRRAGEELEDRLEGVF
                     SDSPEGAHA"
     gene            6081..7004
                     /gene="bchL"
                     /locus_tag="PKV56_13105"
     CDS             6081..7004
                     /gene="bchL"
                     /locus_tag="PKV56_13105"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) iron-sulfur ATP-binding protein"
                     /protein_id="HOX68796.1"
                     /translation="MSTTTVPIPDIKNPGVRKNLRLDGEGSVQVELDPSVKIGNAKVF
                     AIYGKGGIGKSTTSSNLSAAFSKLGKRVLQIGCDPKHDSTFTLTKKMLPTVIDVLETV
                     NFHAEELRVEDFVFPGYNGVMCVEAGGPPAGTGCGGYVVGQTVKLLKEHHLLEDTDVV
                     IFDVLGDVVCGGFAAPLQHADRALIVTANDFDSIFAMNRIVQAIGAKAKNYNVRLGGV
                     IANRSRETDQIDKYNERIGLKLAAHFPDLDVIRRSRLKKSTLFEMEPSPELEAVQKEY
                     MRLAASLWLGTEPMNAVPMKDRDIFDLLGFD"
     gene            7036..7749
                     /gene="bchM"
                     /locus_tag="PKV56_13110"
     CDS             7036..7749
                     /gene="bchM"
                     /locus_tag="PKV56_13110"
                     /EC_number="2.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046406 - magnesium protoporphyrin IX
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium protoporphyrin IX methyltransferase"
                     /protein_id="HOX68797.1"
                     /translation="MNPTSYVERRGKIEDYFDRTAVQAWARLTSDAPVGRIRATVRAG
                     RDRMRATLLDWLPADLRGMRLLDAGCGTGALAVEAAHRGAQVLAIDLSPTLVDLARDR
                     QPAELTSEFSAGRIDFRSGDMLDPALGEFDYVVGMDSLIHYRTPDAVRVVAGLAERTR
                     CGLMFTYAPSNPALIAMRAVGRMFPRSDRAPWIEPVAEAALHRLLAADPALQDWSRGR
                     TQRISSGFYTSQALELVRQ"
     gene            7746..9191
                     /locus_tag="PKV56_13115"
     CDS             7746..9191
                     /locus_tag="PKV56_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HOX68798.1"
                     /translation="MRSKAPSLLIIRARQWLDVAPQFLPFADAATNELPLGRLLRLSL
                     FQVSVGMASVLLIGTLNRVMILEMGMAAWLVSLMVALPLVFAPFRALVGFKSDTHRSV
                     LGWKRVPYIWIGTWLQFGGFAIMPFALILLSGDTRAGPWLGYVSSALAFLLVGAGSQI
                     TQTAGLALATDIAPEGSRPRVVALMYVMLLTGMVASSLIFGALLADFSQVRLIKVVQG
                     AAVVTIALNLIALWKQEVRNPSGTARNLPRPEFRATWQAFARQGRTLRFLIAVGLGTA
                     AFSMQDIILEPYGGEILGLSVSATTLLTALLAGGALAAFGLAARWMNAGIDAYRLAAF
                     GALTGVVAFSAVIFAAPLESAALFRVGTTLIGFGGGLFAVSTLTAAMSLEAPGRAGLA
                     LGAWGAVQATAGGIAIVLGGALRDLVTSMASQGTLGAGLSDASVGYSVVYHIEIALLF
                     ATLVAIGPLVRTSVAPRSQSVSKFGLAEFPG"
     gene            9224..10000
                     /gene="puhA"
                     /locus_tag="PKV56_13120"
     CDS             9224..10000
                     /gene="puhA"
                     /locus_tag="PKV56_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit H"
                     /protein_id="HOX68799.1"
                     /translation="MGTGAITGYIDVAQIVLYLFWIFFFGLVYYLQMESKREGFPLET
                     VYPSGEIRTSPGFVMMPTPKTYLLPHGGTLTVPNDSVSPQTLHAEPLHRLMGAPLVPT
                     GNPMLDGVGPGSYADRADVPDYTWEGAPKIVPLRVATEYGVASRDDDPRGLEVMGADG
                     AVGGTVNDLWIDKSEMVFRYIEVAVPAGGATRNVLLPMNFARIKPDGVKVASILGHQF
                     AQVPATKSPDQVTLLEEEKVMAYYGGGTLYAEPSRQEPLV"
     gene            9997..10758
                     /gene="puhB"
                     /locus_tag="PKV56_13125"
     CDS             9997..10758
                     /gene="puhB"
                     /locus_tag="PKV56_13125"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HOX68800.1"
                     /translation="MKVNYDGHEHEHEFEPEYGLPEPLPAGEHVLWQGAPDWRTLARH
                     VFHVRTLVAYFAVILVIRATVVAMNGGTGAEVIRAVALLSPLAALAVGIAAGMALLCA
                     RTTVYTITDKRVAMRIGIVLNLTFNLPLSRIAMADLREIARGTGDIPLALSGSDRIAY
                     AHLWPHARPWRLARPEPMLRCIPDVARVAAILKQAWTQDQVRVASVQAVAPVAAPRHQ
                     DLPSAPTTQPTPAVKPAPRNRWFADGQDQRLSQGA"
     gene            10769..11212
                     /gene="puhC"
                     /locus_tag="PKV56_13130"
     CDS             10769..11212
                     /gene="puhC"
                     /locus_tag="PKV56_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HOX68801.1"
                     /translation="MAADHMLPRGALLAIGGVLLACVVAVAAVRLSGVTIREPDEAAV
                     AMRELRFEDRADGSVIVIDAPSQTVIHTMVGENGFFRGALRGLARERKRNGLGSEQPF
                     QLIGRADGRLTLVDPATGQRIDLESFGPTNAAVFARFMTDAPATR"
     gene            11245..>12174
                     /gene="acsF"
                     /locus_tag="PKV56_13135"
     CDS             11245..>12174
                     /gene="acsF"
                     /locus_tag="PKV56_13135"
                     /EC_number="1.14.13.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
                     /protein_id="HOX68802.1"
                     /translation="MTTIAQRAPNEATTTALRNTMLTPRFYTTDFDAMDRIDVSPVRA
                     QWDTLMEEFRADSNTDHFQRPADMAGNYAQLPPALHKEFMEFMISSVTSEFSGCVLYT
                     EMKKRVNNPDIKELMSLMARDESRHAGFINQWLKDFGIGVDLGFLVKAKKYTYFRPKF
                     IIYATYLSEKIGYARYIAIYRQVERHPELCFHPIFKWFERWCNDEFRHGEAFALLMRA
                     NPKLLSGHNKLWIRFFLLAVYATMYVRDHQRPEMHAAMGLKPTEYDYSVFRITSEITK
                     QVFPLTLNIDDPRFREGMERMRKISVATEAARVQ"
BASE COUNT         2208 a         3993 c         3867 g         2106 t
ORIGIN      
        1 cggtccgaac acgaagttcc tgctggctca acccttccta gccgacaccg cacgcgcgct
       61 cgagtcgtgc ggcgcccagc gtctggctgc cccgttcccg ctcggcgtcg aaggcacgac
      121 aagctggctt caggccgcgg ccaccgcatt cgggatagct gccgccacgt tcgatcgggt
      181 caccgcgcca ggtcgcgatc gcgccgccac agcacttgcg cggcatcgcg tccagctcgc
      241 cggaaagcgc gtgttcttct ttccagactc tcagcttgaa gtacccatcg ctcgcttcct
      301 gtcgcgcgaa ctgggcatgc aattgataga ggttggcacg ccctacctgc accgtcagca
      361 cctggccgaa gaactcgatc tgctgccagc cggcacattc ctgtccgagg ggcagcatgt
      421 cgataaccag ctcgatcgct gcaggcaggc gcagcccgac atcgtggtct gcggcctggg
      481 gctggccaac ccgctcgaag ccgaagggat gaccacgaag tgggcgatcg aactcgtgtt
      541 cacgccgatc cacggctacg agcaagcggc agatctggcc gagctctttg ctcggcctct
      601 ggtccgtcgc atgaagctcg tcgcatgaag ccgcaccgca cggccagcac ggaaggcacc
      661 agaccatgca gttgacgctc tggacctacg aggggccgcc gcacgtgggt gccatgcgca
      721 tcgccaccgc gttgaaggac gtgcactacg tgctgcacgc gccccagggc gacacctacg
      781 ccgacctgct cttcacgatg atcgagcgcc tgcctaagcg gccgccggtc acctacacca
      841 ccttccaggc gcgagatctg gggggcgaca ccgcagaact gttcaagacc gcagcccgcg
      901 aggcctacga acggttcaag cccaaggcga tgatggtcgg cgcctcttgc accgctgaat
      961 tgatccagga cgatccgggt gggctctgca aggcattgaa cctgcccgtg ccggtcgtcg
     1021 cgctggaatt gccctcgtac cagcgcaagg agaactgggg cgcctcggaa accttctatc
     1081 agatggtccg agcgctggca ggcccgaacg caccggctcc cgggaccgcg aagcccggcc
     1141 gggtcgctgg ggcacgcccg cgctgcaaca tcctcggccc ggcagcactc ggtttccggc
     1201 accgcgatga cttgcgcgag atcaccagcc ttctggcgca actgggcatc gaggtcaacg
     1261 tcgtggctcc gctcggtgcc acggccgacg acctgtcccg actcggcgac gccgatttca
     1321 atgtggtgct ctaccctgag attgcgtcgc tgaccgcatc gtggctgacg cgcatcttcg
     1381 gccaaccggc aaccaagacg atcccgatcg gtgctggcgc cacccgcgaa ttcatcaagg
     1441 aagtggcgca gctggccgac atcgacccga gcgccatgct ggcgaacagc gattcgcgct
     1501 cgccgtggta cgccaagtcc gtcgactcga cctacctgac caacaagcgc gtgttcgtgt
     1561 tcggggacgc gacccatgcg gtcgccgcag cgcgggtggc cagccaggaa ctggggttca
     1621 aggtggtcgg catcggcacc tacagccgcg aattcgcccg tgaggtgaga gaagcggcca
     1681 agctgtacga cgtcgatgcg ctcatcagcg acgactatct cgagatcgag gcgcggatat
     1741 ccgaactgcg tcccgaactg gtgctgggca cgcagatgga gcgccacatc gccaagcggc
     1801 tcggactgcc ttgcgcagtc atctcggcgc cggtgcatgt gcaggacttt ccagcacgct
     1861 actcgccgca gatggggttc gagggcgcca acgtgctgtt cgacacctgg gtgcacccgc
     1921 tgatgatggg tctcgaggag cacctgatcg gcatgttccg cggcgatgcc gagttccacg
     1981 aagacgcggg cccctcgcat ctcgggggcc acgccgcccc gcgggcccat gcgtccgggc
     2041 caggcattgt cgaatcggaa acgctcggtg ccgagctcgt tgcgggcgca gagaaacaga
     2101 ttgaccagaa cgcagaaagc cccgcggccg cgatcccggc aagttgggcc cctgatgcgg
     2161 agaaggagct tcgaaagatt ccgttcttcg tgcgcggcaa agcgcgccgc aacaccgagc
     2221 gatacgcaca agaacatagc gtaccggtga tcaccgtcga gacactgtat gacgcaaaag
     2281 ctcatttcag cagctgaagt ccgatcgaag atccaccaca cgccgatccg ggtggtggtg
     2341 gtgacgatgg acacgcatct ggccagctcg acgcagcgcg cgcgcgccac gttggcacgc
     2401 gagctgcccg gcctgcagtt gtcgatgcac gcggcctccg agtacgcagg caactcggcg
     2461 gccctggaaa ggtgccgggc ggacatcgcg caggccgaca tcgtcgtcgc cggtatgctg
     2521 ttcctggagg atcacttcct cccgatcctc cctgacctgc tggcgcgtcg cgaccactgt
     2581 gacgccatga tctgcatggc ttcggctggc gaaatcgtca agctcacccg cctcggcaac
     2641 ttcgacatga ccaagccctc cagcgggccg atggccttgc tgaagaagct gcgaggcaac
     2701 aaggaaaagt ccgcgcccgg tggcgccgcg cagatgaaga tgctgcgtcg catcccccag
     2761 atgctgaggt tcattccagg cacggctcag gacgtgcgcg cctatttcct gacgctccag
     2821 tactggctcg gcggctccga cgagaacatg ctcaatctgg tgcgccacgt ggtcgaccgc
     2881 ggagcagacg gcccgcgtcg cgttctgcgt ggcctggtga aggtgtccgt gccagtggag
     2941 tatcccgagg tcggcgtgta ccaccctcgc atgccaggtc gcctgtcggc cgacgcatcg
     3001 gcattgccga agccgccgtg caaggaaatt cgcggcacgg tcggcgtgct ggttctgcgc
     3061 tcctacctgc tgtccaacaa cgcggggcac tacgacggcg tgatctcggc cctcgagtcg
     3121 cgcggcctgc gggtgatccc ggccttctcc gccggccttg acgcgcgacc tgccatcgac
     3181 cacttcttca ggaaggatgc agagatcctc gtcgacgcgg tcgtctcgct gaccggcttc
     3241 tcgctggtcg gtggccccgc ctacaacgac gccaaggcgg ccgaggaagt gctcgcccag
     3301 ctcgacgtgc cctacgtggc tgcacacccg gtcgaattcc agacactgga ccagtgggga
     3361 gcctcggacc gcggccttct tccagtcgaa agcacgatca tggtggcgat tccggagctc
     3421 gacggatcga gcagcccgat cgtcttcggg ggccggcccg gcgcagccgg cgtgacgtgc
     3481 acggggtgca gccatgcctg caacttccag gaaagcgaca acgcgaagga catgcacgcc
     3541 tgtccggaac gggccgcgat gctggccgcc cgcgtgctca agctggtcgc attgcgccgc
     3601 agcgagcgcg cccagcgcaa ggtcgcgatc gtcatattca acttcccgcc gaatgccggc
     3661 aacatcggca gcgctgcgta cctgtcggtc ttcgagtcgc tgtttcacac gctgacatcg
     3721 atgaaggcgc aaggctacaa ggtcgatctt cccgaaagca tcgatgcact gcgcgacagc
     3781 gtgctgaagg gcaacgccgc tcagcacggt gcagacgcca atgtgcacac gctgatctcc
     3841 gccgaggacc atgtgcgtcg cgagcgctgg ctccaggaga tcgaaggcca atggggcccg
     3901 gcgcccgggc gccaactgag caatggcaac tcgatcctcg ttctcggaaa gcagttcggc
     3961 aacgtgctgg tcggcgtgca gccggcgttc ggatacgaag gcgatccgat gcgcctgctg
     4021 ttcgagaagg gccttgcacc gacgcacgcc ttctcggcct tctatcgcta tctgcgcgag
     4081 gatttcaagg cgcacgccgt gctgcatttc ggcacccacg gggcgctcga attcatgcca
     4141 ggaaagcaaa gcggcctggg cggcatgtgc tggcccgatc ggttgatcga cgacttgccg
     4201 aacatctacc tctatgcatc gaacaatccg tcggagggcg ccatcgccag gcgccggtcg
     4261 ggggccacgc tgatcagcta cctgacccct ccggtggcgc aagccggtct ctaccgcgga
     4321 ctcaacgact tgagagcctc gatcgagcgg tggcgcaccc tggagccacg gtcggccgat
     4381 cgcgcagagc tggcgattct tgtgcagtcg caggcggccg aactggatct tgcaagtgcc
     4441 gaaccgccct gggatgccgc cgagggcggt gacgccgata cccgtgtcgc tcgcctgacc
     4501 gagcagatgc tcgagctcga gtacaccctg atcccccatg gtcttcatgt aacgggccgc
     4561 ccaccgagcg tcgctgaacg ggtcgacatg ctgctcgcga tggccgacgc atcacatggc
     4621 gtgcagcttg agcgcgcctc gattgaggcg ctggtgggtg gtggcagcgc tgagcgggcg
     4681 ctcgagttgg gccacctgcc gcgcgatgag gcgtcgatgg agatgatgcg cgggttggcc
     4741 aaggcggatg cgatgctcgc cgtggacacc gaggtggaag ccatcctgca tgcgctcgat
     4801 ggtcgattca tccgtccggc gccgggagga gacgtcctgc gcacgccggc cgtgttgccc
     4861 acgggcagga acattcacgg gttcgatccg ttccgcatcc ccagcacatt cgcggtcaag
     4921 gatggcttca agcaagctca actgctgatc gaccgacatc tcgcggatgg tcacgggctg
     4981 cccgaatcga tcgcgatggt gctgtggggc agcgacaacc tgaagaacga gggggcgccc
     5041 atagcgcagg ccctggccct gatgggcgcg ttgcctcgct tcgacagcta cgggcgcatt
     5101 gccggggcga cgctgattcc gctgtccgaa ctggggcgtc ctcgcatcga cgtggtgatc
     5161 acgttgtcag gcatcttccg ggacctcctg cccctgcaga tcaagctgct ggccgaagct
     5221 gcctacctgt gtgcatcggc tgcagacgag ccgatcgaga tgaactggat ccgcaagcac
     5281 gcccttgcct atcagcagga gcatggctgc accctggaaa cggcagctct gcgcgtctac
     5341 ggcaatgccg agggcgccta tggatcgaac gtcaacaacc tcgtcgagag cggcaagtgg
     5401 tcggacgaag gcgagatcgc cgagacctac acccgtcgca aaggctttgc gtacgggcgg
     5461 acaggccgtg ctgttcagca gacggccctg ctcaagagcg cgttggccga cgtccagttg
     5521 acctatcaga acctcgactc ggttgaactg ggcgtgacca cggtcgacaa ctacttcgac
     5581 acgctgggcg gaataacgca agcggtgaag gtggccaagg gcggccgcag cagcgacgtg
     5641 ccacctgtct acatcggtga ccagacccgg ggcgacggcg ccgtgcggtc actgcgggaa
     5701 caagttgcac tggaaacccg gacccgcatg ctgaacccga agtggtacga aggcatgctc
     5761 gaacacggct acgaaggagt gcgccagatc gaggtgcatg tgaccaacac gatgggatgg
     5821 tccgcaacca cgggtcaggt tcaaccgtgg gtctatcagc aactcagcga aaccttcatg
     5881 ctggatcccg agatgcgaga gcgtctcgca aagctgaatc ccacggcctc ggtgcgcgtc
     5941 gccaaccgac tgcttgaagc atccgcgcgc aactactggc aacccgacgc agcaacgctc
     6001 gacgcattgc gtcgtgccgg tgaagaactc gaggaccgac tcgagggcgt gttttccgac
     6061 tctcccgaag gagctcacgc atgagcacga cgacagttcc catcccagac atcaagaacc
     6121 cgggcgttcg caagaatctc cggctcgacg gcgagggcag cgttcaggtc gaactcgatc
     6181 cgagcgtcaa gatcggcaac gccaaggtct tcgcgatcta cggcaagggc ggcatcggca
     6241 agagcaccac atcgagcaac ctgtcagcgg cgttctcgaa gctgggcaag cgagtgctcc
     6301 agatcggctg cgatccaaag catgactcca ccttcacgct gacgaagaag atgctgccca
     6361 ccgtcatcga cgtgctcgag acagtcaact tccatgcgga agaactgcgc gtcgaggact
     6421 tcgtcttccc cggatacaac ggggtgatgt gcgtcgaggc cggcggaccg cccgccggga
     6481 ccggctgtgg cggctacgtc gtcggacaga cggtcaagct gctgaaggag caccacctgc
     6541 tcgaagacac cgacgtggtg atcttcgacg tgctcggcga tgtggtgtgc ggcggattcg
     6601 ctgcaccgct gcagcacgcc gaccgcgcct tgatcgtcac cgccaacgac ttcgattcga
     6661 tcttcgcgat gaaccgcatc gtgcaggcca tcggtgcgaa ggcgaagaac tacaacgtgc
     6721 ggctgggcgg cgtgatcgcc aaccgcagcc gcgagaccga ccagatcgac aagtacaacg
     6781 agcgcatcgg tctgaagctc gcagcgcact tcccggacct ggacgtgatc cgccgcagcc
     6841 gcctgaagaa gtcgaccctg ttcgagatgg aaccctcgcc cgagctcgag gccgtgcaga
     6901 aggagtacat gcgactggca gcctcgctct ggctgggcac cgagccgatg aatgccgtgc
     6961 cgatgaaaga ccgcgacatc ttcgatctgc tgggtttcga ctgatgccga accacaccgc
     7021 accacggaag ctcacatgaa tcccaccagc tacgtcgaac gccgcggcaa gatcgaggac
     7081 tatttcgatc gcaccgccgt gcaggcctgg gcccggctca cgtccgacgc cccggtcggc
     7141 cgcattcgcg ccacggtacg cgccggtcgc gatcgcatgc gcgccaccct gctcgactgg
     7201 ctgcccgcag acctgcgcgg catgcgcttg ctcgatgcgg gttgcggcac aggagccctc
     7261 gcagtggagg ccgcacaccg cggggcacag gtgctggcga tcgatctgtc tcccacgctg
     7321 gtcgatctgg cacgcgaccg ccagcccgca gagctgacca gcgagttcag cgccggtcgc
     7381 atcgactttc gctcgggaga catgctcgac ccggcgctgg gcgaattcga ctacgtcgtc
     7441 ggcatggact cgctgatcca ctaccgcacc ccggatgccg tgcgcgtggt cgcgggtctg
     7501 gccgagcgca cgcgctgcgg cctgatgttc acgtacgcgc ccagcaatcc ggcgttgatc
     7561 gcgatgcgcg ccgtgggacg catgttcccg cgcagcgatc gcgcgccctg gatcgaaccg
     7621 gtggccgagg ctgcattgca ccggctgctg gcagcagatc ccgcgctgca ggactggagc
     7681 cgcggccgca cgcagcgcat ctcaagcggc ttctacacat cgcaggcact ggaactggta
     7741 cgccaatgag gtcgaaggcg ccatcgctgc tgatcattcg agcccggcaa tggctcgatg
     7801 tggcgccgca gttcctgcct ttcgcggacg cggccacgaa tgaactgccg ctgggcaggc
     7861 tgctgcgact gtcgctgttc caggtttccg tgggcatggc cagcgtcctg ctgatcggga
     7921 cactgaaccg cgtgatgatc ctcgagatgg gcatggcagc ctggctggtg tctctgatgg
     7981 tcgcgctgcc cctcgtgttt gcaccatttc gcgcattggt cggcttcaag tccgatacgc
     8041 atcgctcggt gctgggctgg aagcgggtgc cctacatctg gataggcacc tggctccagt
     8101 tcggaggctt cgcgatcatg ccgttcgcct tgatcctgct gtcgggcgac acgcgggccg
     8161 gcccgtggct cgggtacgtg agctcggcgc tggccttctt gctcgtcgga gcgggttctc
     8221 agatcacgca gaccgctggc ctggctctcg ccacggacat cgcacccgaa ggatcacggc
     8281 cacgtgtggt ggcgctgatg tacgtgatgc tgctgaccgg catggtggca agcagcctga
     8341 tcttcggtgc cctgctggcc gacttcagtc aggtgcggct gatcaaggtg gtgcagggtg
     8401 ccgctgtggt cacgatcgca ctgaacctga tcgcgctgtg gaagcaggag gttcgcaacc
     8461 cgtccggtac ggccagaaac ctcccgcgcc ccgagttccg cgccacctgg caggcctttg
     8521 ccagacaggg acgcacactg cggttcctga tcgccgtggg cctgggaact gccgccttca
     8581 gcatgcagga catcatcctc gagccctacg gcggagaaat cctcgggctg agcgtcagcg
     8641 ccaccacctt gctgaccgcc ctgctggccg gaggcgcact ggctgcattc ggccttgctg
     8701 cacgctggat gaacgctggc atcgatgcct accgtctggc agcctttggc gctctgacgg
     8761 gcgtcgtcgc attctcggcg gtcatctttg cggcgccact cgaatcggct gctctgtttc
     8821 gggtcgggac gaccctgatc ggattcggcg gcggcctgtt tgccgtcagc accttgaccg
     8881 ctgcaatgag cctcgaggca ccaggccgcg ccggtctcgc gctgggtgcc tggggcgccg
     8941 tgcaggcgac cgcaggtggc atcgccatcg tgctgggcgg tgcattgcgc gacctagtca
     9001 ccagcatggc atcgcaggga acactgggcg ccgggctgtc cgatgcgtcg gtcggctaca
     9061 gcgtcgtgta tcacatcgaa atcgcactgc tgttcgccac gctggttgcc atcggtccgc
     9121 tcgtccgcac gtcggtcgcg cctcgatcgc aatctgtttc gaagtttggg ttggccgaat
     9181 ttcccggctg accagttctt gctctaaaga aggagaccac accatgggta ctggagccat
     9241 caccggctac atagacgttg cacagatcgt gctctatctg ttctggatct tctttttcgg
     9301 tctggtgtac tacctgcaga tggaaagcaa gcgcgaggga ttcccgcttg aaaccgtcta
     9361 tccctcggga gagatccgca ccagtccggg cttcgtgatg atgccgacgc ccaagacata
     9421 tctgctgccg cacggtggca cgctgacggt tccgaatgac agcgtttctc cacagaccct
     9481 gcacgccgaa cccctgcaca ggttgatggg agccccgctc gttccgacgg gaaatcccat
     9541 gctcgatggt gtgggacccg gctcgtacgc tgaccgggcc gacgtgcccg actacacgtg
     9601 ggaaggcgcc ccgaagatcg ttccgttgcg cgtggccacc gaatacggcg tggcgtccag
     9661 ggacgacgac ccgcgaggcc tcgaagtcat gggagccgac ggtgcagttg gcggcacggt
     9721 caacgacctg tggatcgaca agtcggaaat ggtcttccgt tacatcgaag tggcggtgcc
     9781 cgccggcggc gcaacgcgca acgtgcttct gcccatgaac ttcgcacgca tcaagcctga
     9841 cggcgtgaag gtggcctcga tactgggcca ccagttcgcg caggtaccag caacgaaaag
     9901 cccggatcag gtcaccctgc tcgaggaaga aaaggtgatg gcgtactacg gcgggggcac
     9961 cctgtacgcc gagccatcgc gccaggaacc actggtatga aagtgaacta cgacgggcac
    10021 gaacacgaac acgagttcga gcccgagtac ggcctgcccg agccgctgcc cgcgggcgag
    10081 cacgtgctct ggcagggtgc cccggactgg cggacgcttg cacgacatgt ttttcacgtg
    10141 agaacgctgg tcgcgtactt cgcagtgatc ctcgtgatac gcgccactgt cgtcgcgatg
    10201 aacggtggga ctggtgcaga ggtgatccgg gcagtcgcgt tgttgtctcc gctggcagct
    10261 ctggccgtcg ggatcgcggc gggcatggct ctgctctgcg caagaaccac ggtctacacg
    10321 atcacggaca agcgggtcgc gatgcgtatc ggcatcgtcc tcaacctgac cttcaacctg
    10381 cctttgagcc gcatagcgat ggccgatctg cgtgaaatcg cccgtggaac cggagacatc
    10441 ccactggcgc tgtccggctc cgatcgaatc gcctacgccc acctgtggcc gcatgcgcgg
    10501 ccctggcgcc tcgctcgtcc ggaaccgatg ctgcgctgca ttcccgatgt cgcccgcgtg
    10561 gctgcaatcc tgaagcaggc gtggacacaa gaccaggtcc gcgtcgccag cgttcaggcc
    10621 gtcgcgccgg ttgccgcacc tcgccaccag gacttgcctt cagcaccgac aacccagccg
    10681 acgccagccg tgaagcctgc ccctcgcaat cggtggtttg ccgacggcca ggaccagcgt
    10741 ctgtcccagg gggcctgagg tgggagccgt ggctgcggac cacatgctgc ctcggggcgc
    10801 cttgctggcc atcggcgggg tgctgctggc ctgcgtcgtc gccgtggctg ccgtgcggtt
    10861 gtccggcgtc accatccgtg agccggatga agctgcagtg gccatgcgcg agttgcgatt
    10921 cgaggatcgc gccgacggca gcgtgatcgt aatcgatgcg cccagccaga cggtcatcca
    10981 caccatggtc ggcgagaacg ggttctttcg gggcgctttg cgcggcctgg cccgggaacg
    11041 caagcgcaac ggcctcggtt cggagcaacc tttccaattg atcgggcggg ccgacgggcg
    11101 cctgacactg gtcgacccgg ccacaggcca gcgcatcgac ctggaatcct tcgggcccac
    11161 caatgcggcc gtgttcgccc gattcatgac tgacgcacca gctactcgct gaggcatcga
    11221 taaccagaag ttccggagca gaagatgacc acgatcgccc aacgcgcccc caacgaagcg
    11281 accaccactg cgctgcgcaa cacgatgctg acgccgcgct tctacacgac tgatttcgat
    11341 gcgatggacc gcatcgatgt gagcccggtc cgagctcagt gggacacgct catggaggaa
    11401 ttcagggctg actccaacac cgatcacttc cagcggccag ccgacatggc gggcaactac
    11461 gctcaacttc cgccggcgct gcacaaggag ttcatggagt tcatgatcag ttcggtgacc
    11521 tccgagttct ccggctgcgt gctttacacc gagatgaaga agcgcgtgaa caacccggac
    11581 atcaaggaac tgatgtcact gatggcgcgt gacgagagcc gtcatgccgg cttcatcaac
    11641 cagtggctga aggacttcgg catcggcgtg gatctcggat tcctggtcaa ggcgaagaag
    11701 tacacctact tccgcccgaa gttcatcatc tacgcgacct acctgtccga gaagatcggc
    11761 tacgcccgat acatcgcgat ctatcgtcag gtggagcgcc atcccgagct ctgcttccat
    11821 ccgatattca agtggttcga gcgctggtgc aacgacgaat tccgtcacgg tgaggcgttt
    11881 gcgctgctga tgcgagccaa ccccaaattg ctgagcggcc acaacaagct gtggatccgc
    11941 ttcttcctgc tggcggtcta cgccacgatg tacgtgcgcg accaccagcg cccggaaatg
    12001 cacgcggcca tggggttgaa gccgaccgag tacgactaca gcgttttccg catcacttcg
    12061 gaaatcacca agcaggtgtt ccccctgacg ctgaacatcg acgatccgcg attccgcgaa
    12121 ggcatggagc gcatgcgcaa gatctcggtg gccactgaag ccgcccgggt ccag
//
LOCUS       DAOORV010000105        12092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6523_length_12092_cov_7.359143, whole genome shotgun sequence.
ACCESSION   DAOORV010000105 DAOORV010000000
VERSION     DAOORV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6523_length_12092_cov_7.359143"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PKV56_13140"
     CDS             <1..1163
                     /locus_tag="PKV56_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOX68803.1"
                     /translation="LGSFFAGTVATILLALFAPPLADLALKFGPAEYFSLMVLGLVAS
                     VVLASGSLLNAIGMVLLGLLLGLAGTDVNSGVARYTFDLPELAEGINFVIVAMGMFGI
                     GEIVRNLEHDQTRNLVMKKVSGLMLSKDDFKRIMAPVLRGTFLGSALGILPGGGAMLA
                     SFAAYSIEKKVSKNSAQFGKGAIEGVAAPESANNAGAQTSFIPMLTLGIPSNPVMALM
                     IGAMIIQGIQPGPAVMTEQPALFWGLIASMWIGNFFLVVLNLPLIGLWVKMIMVPYHR
                     LYPAILMFCAIGVFSLNSSQFDIYLMALFGLFGYVCAKLNCEPAPMLLGFIIGPMMEE
                     YLRRAMILSKSDPMVFLQRPISAVMLAMAALALFVVLSPTMQKKREEAFHEE"
     gene            1362..2141
                     /locus_tag="PKV56_13145"
     CDS             1362..2141
                     /locus_tag="PKV56_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOX68804.1"
                     /translation="MLPVLNISAYRFVVLDDVDALRAQIHAQAASRGLKGTVLLAEEG
                     INLFLAGPPEAVRDFVSWLRRSDARFAALEAKESLSESVPFGKLLVKVKREIIRMNHP
                     AIRPQAERAPAVSARTLARWLDQGHDDEGREVVTLDTRNAFEVDAGRFRGAIDWRIDK
                     FSDFPAAVLAHRDDFRGKTVVSYCTGGIRCEKAALFMADAGIERVMQLDGGILKYFEE
                     TGGAHYDGHCFVFDARREVASDLRPAGAAMPHDPEGIVDPV"
     gene            2190..3548
                     /gene="egtB"
                     /locus_tag="PKV56_13150"
     CDS             2190..3548
                     /gene="egtB"
                     /locus_tag="PKV56_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HOX68805.1"
                     /translation="MQPPPPANSERTRVADATQSELASRYTAVRAATLRLAEPLSDED
                     CQVQSMPDASPTKWHLAHLTWFFETFVLEPHEPGFQAFDPRFRVLFNSYYQGVGEQRP
                     RAQRGLISRPSLATVKRYRAQVDERMQALMARAVAEGTLDADLHGLITLGLHHEQQHQ
                     ELLLTDIKHVLSFSAPSATESMAYARRWPIGAVQAQPLRWFAYEGGLFELGHDAALDG
                     LFCFDNETPRHREYTAPFELASRPANHGEFLAFVEDGGYRRPELWLSMGWSWVNEGPH
                     GQPRQAPLYWQQRDGHWFTHTLQGTVAIDPHTPMCHLSYFEADAYARWAGARLPTEGE
                     WEFAARRLGPRAMQHANFADRGAFHPLPPIAAASEVPLQMYGDVWEWMQSNYNPYPGY
                     RPLPGAVGEYNGKFMCNQFVLRGGSCATPVGHVRASYRNFFPPEAQWQFSGLRLARNL
                     AA"
     gene            complement(3558..4262)
                     /locus_tag="PKV56_13155"
     CDS             complement(3558..4262)
                     /locus_tag="PKV56_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HOX68806.1"
                     /translation="MDVALTVFRLLPWHDWAALSTFFCGWVGYALFARKQAGIKPSIL
                     AATNRIRRQWMLQTTYREVRVIDGVVVQSLSTSPSFFASTTILIIGGLLAVLGSSNAS
                     ELVREIPFAARTTVLVFDLKIVLMLAIFVYAFFRFTWSMRQYTFGALLVASAPEAEQF
                     QSLALSRDEFADRAGRVVGLAAETFNDGLRAYYFAFAAVSWFLSPLAFMLATGGVIYI
                     LYQREFHSDVLVVLNS"
     gene            complement(4325..4879)
                     /locus_tag="PKV56_13160"
     CDS             complement(4325..4879)
                     /locus_tag="PKV56_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOX68807.1"
                     /translation="MSKIAIVFHSGYGHTKKMAESVAAGAAGELVAIDAEGNLSDAQW
                     ATLLAADAIVFGSPTYMGSVSWQFKKFADASSKPWFTQLWKDKIAAGFTNSASMNGDK
                     LSTLHYMFTLSQQHSMIWVGAGMMPSNSKAATRNDINYVASFAGAMASTPSDASVDEM
                     LPGDLETARLFGERIAAVTARFNR"
     gene            complement(4937..5638)
                     /locus_tag="PKV56_13165"
     CDS             complement(4937..5638)
                     /locus_tag="PKV56_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOX68808.1"
                     /translation="MLTLRKSEDRGHADHGWLQSFHSFSFADYRDPDQMGWGNLRVIN
                     DDYIAPGTGFGTHGHRDMEIVTYVLSGALAHKDSLGTGAVILPGEVQRMSAGRGVQHS
                     EFNHAPADRTHLLQIWILPREQGIEPSYEQKQFDAADKRGRLRLVASPDGRDGSVTVH
                     ADASIRAGLFDAGERAELALDPQRKAYVHLVRGRLSVNGQALVGGDALKISDESRLVI
                     DDAREAELLVFDLTA"
     gene            5762..6850
                     /locus_tag="PKV56_13170"
     CDS             5762..6850
                     /locus_tag="PKV56_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX68809.1"
                     /translation="MPRNLLTPDALTMMDVIARTGSFAAAARELGKVPSALTYNVRQL
                     EDALDVLLFDRRSGQAQMTAAGEELLYEGRRLLEQMDAVANRVKRVACGWETQLSIAV
                     NDLMSRPTMFELCEHFFAMRTGGPADQELKRRAREKGAVATPVGAPPADDTGPGTRLR
                     LRTEVLAGTWEALITGNADLAVGVDLGLSTPSGVKVEVLGSMGFVFAVAPHHPLATHE
                     EPVTDSVRMRYRAVAVADSAQRLAPITINVLPGQDVFTVPTMQSKIEAHLRGLGCGYL
                     PEPIAREHIAAGRLIVKTLERVQQPVQFGYAWRSPTLTQPAIAEIAGARVAPPSGLAL
                     RWWLDRLESPATRLALLERHTGLRPMNG"
     gene            6874..7818
                     /gene="gluQRS"
                     /locus_tag="PKV56_13175"
     CDS             6874..7818
                     /gene="gluQRS"
                     /locus_tag="PKV56_13175"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOX68810.1"
                     /translation="MRPTGRFAPSPTGPLHAGSMVAALASWLDARAHGGRWLVRIEDV
                     DMPRCVPGADEFILLQLASCGLISDDPVVWQSQRHDLYLSAMRRQLMRHQRSFPCSCS
                     RRDIEQAWLAHGQVRRDHGELVYPGTCRQGLHGKPARAQRLRCTQEDGSDLVIAWTDR
                     RLGAMTQNVTQAIGDFVLRRADGLWAYQLAVVVDDADQGITHVVRGGDMAESTPRQIH
                     LQHELGVPTPAYLHTPVVMAADGHKLSKQNGAEAFDPREPLKALRDAGTVLGIEVQGE
                     TVADWLASAVPAWAALYPPTLPEPPTQQGAPDRPAPIG"
     gene            8091..8813
                     /locus_tag="PKV56_13180"
     CDS             8091..8813
                     /locus_tag="PKV56_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX68811.1"
                     /translation="MTPAPTGRRQALAWAAAMLAMPAVAQSPATLTISTNNTPLDRKA
                     LAQLALEACSRIGVGFKLVSLPSERSLQAANQGEVDGEGLRVAGLEETYPNLLRVPER
                     FIGISFVAFASNPAITLTEGWASLKPHRVAFINGWKMFETHATGARVVHKLDKAEQLF
                     QMLAAGRIDLALYTLADGVALVRQLGLSGIAPLTPALRDVDMFLYLHRRHQNLVLRLA
                     AALREMKTDGSHQRILASTGPD"
     gene            8810..>12092
                     /locus_tag="PKV56_13185"
     CDS             8810..>12092
                     /locus_tag="PKV56_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX68812.1"
                     /translation="MSPGTEPGPASLLRLGLASRVSRRILAWALAVGAVGTLAVSWWD
                     AERAYQDQTARLGTSLQGLAEFAAPSLAVGAWTFDRTQIDTQLSAFTRMADVSAVTLA
                     ITGQPPVRLAPHPISSLVLERKVPLVYVDDGRTQTLGTLTLTHDLQPLQAQRRSQWLH
                     AMATNGLVILLTALSSVLIYQFIVTRRLLAIAHQLRGVTAQDLRSLPPEPPLPVRKAP
                     DELDDLAASIATLQATGRQALLAGDDEHALLRSLMDTIPDLVWLKDVNGKFLACNPRF
                     EQLIGAGESQILGKDDYAFMPRDEADFFRDNDRRAIEAGGSSVNEEWLDFKADGYRGL
                     FETIKTPMRLPDGRLVGVLGIAREVTKQRAAVEALRDREELYRSIVSRAADGILLVDP
                     ETGALEEFNDAACLQLGYTREEFAQLNVFELQADFSPADVRRSFETTRTEGGSRFEHR
                     HRRKDGSLRSAWVSTSPVTVRGRTLITVLWHDISSRIEAEAAVQDERRMRETFLESIP
                     GVFYGIDTSSRLVLWNRNFELAFERSAQQLTGLPAADLFEGEDRLRIAQNIAKVFAEG
                     HTEVEADFRTPGGRHAAYLFTGRLVEIGGQPMLVGVGVDISARREAEQALKGLNSQLE
                     QRVVARTADLRRAHEQLLDTHFAMDSVGIGIAWADSETGRFVYVNRHIADFLGYSVDE
                     MLSLGVSDIDPAFPTHDFPAHAQAVREAGHLQFETSHRTRDGRLLPVEMSVYYHAGNS
                     RSGPRIIGFMTDIARRKEFEQALRRSKEEAEAANKAKSTFLANMSHEIRTPMNAIIGL
                     THLMRRNNPTPQQLDWLDKIDGSGRHLLAIINDVLDLAKIEAGRLELESTNFHLSSVL
                     DNVVSIIREPARAKGLDIEIDTADSVPMWLSGDPTRLRQALLNYVGNAVKFTRRGCIR
                     LRTELLEDGPEELLVRFEVDDTGIGIPPEKLAGLFTEFEQADASTTRRHGGTGLGLAI
                     TSRLALLMNGEVGARSVPGEGSSFWFTARLRRGLDVVPGSVGDKPPHEAEPWIQLQRI
                     NARRTARILLVDDNPINREVALQLLDGSGLLVDTADNGAEAVRMAAACPYDLVLMDVQ
                     MPVMDG"
BASE COUNT         2078 a         4069 c         3969 g         1976 t
ORIGIN      
        1 ccctgggctc tttttttgcc ggcactgtag caacgattct gttggcgctc tttgcgccgc
       61 ccttggctga cttggctttg aaattcgggc ctgcagagta tttttctctg atggtgctgg
      121 ggctggtggc ctcggtggtg ttggccagcg gctcactcct gaacgcgatt ggcatggtcc
      181 ttctcggcct gcttctgggc ttggcgggca cggacgtgaa ctcgggcgtg gcgcgttaca
      241 ccttcgactt gcctgaactg gccgaaggaa tcaacttcgt catcgtggcg atgggcatgt
      301 ttggcatcgg cgagatcgtg cgtaacctcg agcacgacca gacgcgaaat ctggtgatga
      361 aaaaagtctc cggtctgatg ctcagcaagg acgacttcaa gcgaatcatg gcgccggtct
      421 tgcgcggaac tttcttgggc tcggcgctgg gcatactccc gggcggtggc gccatgctgg
      481 cctcgtttgc ggcctactcg atcgagaaaa aggtctccaa gaattcggcc cagttcggca
      541 agggtgctat cgagggcgtc gcagcacccg aatccgccaa caacgctggc gcacagacct
      601 cattcattcc aatgctgacg ctgggcatcc catccaaccc tgtgatggcc ctgatgattg
      661 gcgccatgat catccagggc attcagcccg gcccggccgt gatgacggag cagcccgcgc
      721 tgttttgggg gctgatcgcc tccatgtgga tcggcaactt ctttctcgtg gtgctcaacc
      781 tgcccttgat cggcctgtgg gtcaagatga tcatggtgcc ctaccaccgg ctgtatccgg
      841 ccattctgat gttctgtgcg attggcgttt tcagcctcaa cagcagtcag ttcgatatct
      901 acctgatggc gctgttcggc ctgtttggat atgtgtgcgc caagctgaac tgcgagccag
      961 cgcccatgct cctgggcttc atcatcgggc ccatgatgga ggagtacctg cgcagggcga
     1021 tgatcctctc caaaagcgac ccgatggtgt tcttgcagcg gccgatcagt gccgtcatgc
     1081 tggcgatggc cgccttggcg ttgtttgtag tgctgagccc gacgatgcag aagaagcgcg
     1141 aggaggcgtt tcacgaggag tgatcgcgga ttggggtggc tagcagcctg cctcgtccgc
     1201 cgccgctttg cccaacgctt tttgcgccca cggcacctct agcaaggagg gcgtctttcc
     1261 tggcggccag gggagacatg agcgcaacgc ggcggccgcg tccgtcgctc gctacgccat
     1321 ctttttggcc tgaacgggct cccgtcctta cactccccgc cgtgcttccg gttctcaaca
     1381 tctccgccta ccgcttcgtc gtgctcgacg atgtggacgc cctgcgcgcg cagatccatg
     1441 cgcaggccgc ctcgcgtggg ctgaaaggca ccgtgctgct ggccgaggaa gggatcaacc
     1501 tgttcctggc cggaccgccc gaagcggtgc gcgatttcgt gagctggttg cggcgcagcg
     1561 acgcgcgctt cgcggctctc gaggccaagg aaagcctgtc ggagtctgtg ccgttcggaa
     1621 aactgctggt gaaggtcaag cgcgagatca tccggatgaa ccacccggcg atccgtccgc
     1681 aagccgagcg tgcaccggcg gtcagcgcgc ggacgcttgc acgctggctc gaccagggcc
     1741 acgacgacga aggccgggag gtggtcaccc tggacacccg caacgccttc gaggtagacg
     1801 ccggccgctt tcgcggcgcg atcgactggc gcatcgacaa gttcagcgac ttccccgccg
     1861 ccgtgctcgc gcaccgcgac gattttcgcg gcaagacggt ggtgagctac tgcaccggcg
     1921 gcatccggtg cgagaaggct gcgctgttca tggccgatgc cggcatcgaa cgggtgatgc
     1981 agctcgacgg cggcatcctg aagtatttcg aagagaccgg cggcgcacat tacgacggcc
     2041 actgcttcgt gttcgacgca cgccgcgagg tcgccagcga cctgcgtccg gccggtgccg
     2101 cgatgcccca cgatccagag ggaatcgtcg atccggtgta aggtaacttg gtgtcacgtc
     2161 ggcatcacca gctgaacacc agatcgccga tgcagccccc cccgcctgcc aactcagaac
     2221 gtacgcgtgt cgctgatgcg acgcagagcg aacttgcctc gcgctacacc gcggtgcgcg
     2281 cggcgacgtt gcgcctggcc gaaccgctca gcgacgagga ctgccaggtg caatccatgc
     2341 cagacgcgag cccgaccaag tggcacctag cgcacctgac ctggttcttc gagaccttcg
     2401 ttctggaacc gcacgaaccc ggcttccagg ctttcgaccc gcgcttccgg gtgctgttca
     2461 acagctacta ccagggcgtc ggcgagcagc gtccgcgcgc gcagcgcggg ctgatcagtc
     2521 gaccgagtct ggccacggtg aagcgctatc gcgcccaggt cgacgaacgg atgcaggcgt
     2581 tgatggcgcg agcggtggcc gaaggcacgc tcgatgccga tctgcacggc ctgatcaccc
     2641 tcggcctgca ccacgaacag cagcaccagg aactgctgct gaccgacatc aagcacgtgc
     2701 tgtcattcag tgcgccgtca gcgaccgaat cgatggccta cgccagacgc tggccgatcg
     2761 gtgcggtcca ggcccagccg ctgcgctggt tcgcttacga gggcgggctg ttcgagctgg
     2821 gccacgacgc ggcgctcgat ggtctattct gcttcgacaa cgagacgccg cgccaccgcg
     2881 aatacaccgc gccgttcgag ctggcttcgc gcccggccaa ccacggcgag ttcctggcct
     2941 tcgtcgagga cgggggctac cggcggccgg agctgtggtt gtcgatgggg tggagctggg
     3001 tcaacgaagg cccgcacggc cagccgcgcc aggctccgct gtactggcag cagcgtgacg
     3061 gccactggtt cacgcacacg ctgcagggca cggtggcgat cgacccgcac acccccatgt
     3121 gccacctgag ctacttcgag gccgacgctt atgcgcgctg ggccggcgcg cgcctgccga
     3181 cggaaggcga gtgggagttc gcggcacggc gactcggccc gcgtgcgatg caacacgcaa
     3241 acttcgccga ccgcggcgcg ttccatccgc tgccaccgat cgccgccgcc agcgaagtgc
     3301 cgctgcagat gtacggcgac gtgtgggagt ggatgcaatc caactacaac ccctaccccg
     3361 gctaccggcc gctgcccggt gcggtcggtg aatacaacgg caagttcatg tgcaaccagt
     3421 tcgtgctgcg cggcggctcg tgcgcaacgc cagtcggcca cgtgcgggcg agctaccgga
     3481 acttctttcc gcccgaggcg caatggcagt tcagcgggtt gcggctggcg cgcaacctcg
     3541 ccgcctgaag acccgcctca ggaattcagc acgacgagca catccgaatg gaactcgcgc
     3601 tggtacagga tgtagatgac gccgccggtg gcgagcatga aggccagcgg tgacaggaac
     3661 cagctcaccg cggcgaacgc gaagtagtag gcgcgcagtc cgtcgttgaa ggtttcggcc
     3721 gcgagtccga cgacgcgacc cgcccggtcg gcaaactcgt cccgggacag tgcgaggctc
     3781 tggaactgct cggcctcggg cgccgaagcg accagcagcg caccgaaggt gtactgccgc
     3841 atcgaccacg tgaagcggaa gaaggcgtag acgaatatcg ccagcatcag cacgatcttc
     3901 aggtcgaaca ccagcaccgt cgtgcgcgct gcgaaaggga tctcgcgcac gagttcgctg
     3961 gcgttgctcg aaccgagcac cgccagcagg ccaccgatga tcaggatggt cgtcgaggcg
     4021 aagaacgacg gactcgtcga caggctctgg accaccaccc catcgatgac gcgcacctcg
     4081 cggtaggtgg tctgcagcat ccactgacgc cggatccggt tggtcgccgc gaggatcgac
     4141 ggcttgatgc cggcctgctt gcgcgcaaac agcgcgtaac ccacccatcc gcagaagaag
     4201 gtggaaagcg ccgcccagtc gtgccagggc agcagccgga aaacggtcag cgcgacatcc
     4261 atgcgcgaat gtagcaagcg ccgccgacgc ggttcgaacg cgtcggcggc gctgtgtcta
     4321 caggctacct gttgaagcgc gcggtgaccg ccgcgatgcg ctcgccgaac agccgcgcgg
     4381 tctcgaggtc gcccggcagc atctcgtcga cgctggcgtc cgacggcgta gacgccatcg
     4441 ctccggcgaa tgaagccacg taattgatgt cgttgcgcgt tgcagccttg gagttgctgg
     4501 gcatcatccc cgcgccgacc cagatcatgc tgtgctgctg gctcagtgtg aacatgtagt
     4561 gcaaggtcga caacttgtcg ccgttcatcg atgccgagtt ggtgaacccg gcggcgatct
     4621 tgtccttcca gagctgggtg aaccaaggct tgctggacgc gtcggcaaac ttcttgaact
     4681 gccacgacac gctgcccatg taggtcggcg agccgaagac gatcgcgtcg gccgccagca
     4741 gcgtggccca ctgcgcatcg ctcagattgc cttcggcatc gatggcgacc agctcacctg
     4801 ccgcgccggc agcgaccgac tcggccatct tcttggtgtg gccgtagccg ctgtggaaaa
     4861 cgatagcgat cttggacatg gatgctcctg aaagaggatt tgagaacgtg ggcgagcgga
     4921 aagggtcagg cgccggtcag gccgtcagat cgaacaccag caattcggcc tcccgggcgt
     4981 cgtcgatgac cagccggctt tcatcgctga tcttcagggc gtctccgccc accagcgcct
     5041 ggccgttcac gctgagcctt ccgcgcacca ggtgcacgta ggccttgcgt tgcggatcga
     5101 gcgccagctc ggcgcgctcg ccggcatcga acagcccggc gcggatcgaa gcatcggcat
     5161 gcacggtgac agaaccatca cggccgtcgg ggctcgcgac caggcgcagg cgtccgcgct
     5221 tgtcagccgc atcgaactgc ttctgttcgt agctgggctc aatgccctgc tcgcggggca
     5281 ggatccagat ctgcagcagg tgggtccggt cggcgggtgc gtggttgaac tcgctgtgct
     5341 gaacgccgcg accggcgctc atccgctgca cctcgccggg caggatgacc gcgccggtgc
     5401 ccagactgtc cttgtgagcc agcgcgccgc tgagcacgta ggtcacgatc tccatgtcgc
     5461 ggtgaccatg ggtgccgaac ccggtgcccg gcgcgatgta gtcgtcgttg atgacccgca
     5521 ggttgcccca gcccatctgg tccgggtcgc ggtagtcggc gaacgagaag ctgtggaaac
     5581 tctggagcca gccgtggtcg gcatgacctc ggtcctcgga cttgcgcagc gtgagcatgg
     5641 tggtctcctg ggtgcctgac gaatgagttc actgtaggag acccaaggcc aaatggcgtg
     5701 caccgggact tgaagcagac gttcaaataa catgaagctc tgaacacctc ccgcgccgac
     5761 catgccccga aatctgctca cccccgacgc gctgacgatg atggatgtca tcgcccgcac
     5821 cgggagcttt gccgctgccg cacgcgagct gggcaaggtg ccctccgcac tgacctacaa
     5881 cgtgcggcaa ctcgaagacg cgctcgatgt gctgctgttc gaccgccgat ccggccaggc
     5941 acagatgaca gcggccggcg aggaactgct gtacgagggc cgccggctgc tcgagcagat
     6001 ggacgcggtg gccaaccgcg tcaagcgggt ggcctgcggc tgggagaccc agctgagcat
     6061 tgccgtcaat gacctgatgt cgcgccccac catgttcgag ctgtgcgagc acttcttcgc
     6121 gatgcgaacc ggcgggccgg ccgatcagga gttgaagcga cgcgcgcgcg agaaaggcgc
     6181 agtcgcgaca cccgtcggcg cacctccggc cgacgacacc ggccccggca cgcggctgcg
     6241 gctgcgcacg gaagtgctgg ccggcacctg ggaagcgctg atcaccggca acgccgacct
     6301 ggccgtcggc gtcgacctcg gactgtcgac gccgagcggc gtcaaggtcg aggtgctcgg
     6361 ctcgatgggc ttcgtgttcg cagtcgcgcc gcaccatcct ctcgcgacgc atgaagagcc
     6421 ggtgacggac agcgtgcgga tgcgctaccg cgcggtggcc gtcgccgact cggcccagcg
     6481 gctggcgccg atcaccatca acgtgctgcc ggggcaggac gtgttcaccg tgccgacgat
     6541 gcaatccaag atcgaggcgc atttgcgcgg actgggctgc ggctacctgc ccgagccgat
     6601 tgcgcgcgaa cacatcgccg ccggtcggct catcgtcaag acgctcgagc gcgtccagca
     6661 gccggtgcag ttcggctacg cgtggcgcag cccgacactg acacagcccg cgatcgccga
     6721 gatcgccggc gcccgtgtgg cgcccccgag cgggctcgcg ctgcgctggt ggctcgaccg
     6781 gctggaaagc cccgcgacgc gcctcgccct gctggagcgg cacaccggcc tgcggccaat
     6841 gaacggctga gcgcgccggg cccgcgaatg tcgatgcgac cgaccggccg ctttgcaccc
     6901 tcgcccaccg ggccgctgca tgcaggctcg atggtcgcag cgctggcgag ctggctcgat
     6961 gcgcgtgctc acggcggacg ctggctggtg cgcatcgagg acgtcgacat gccgcgctgc
     7021 gtccccggtg ccgacgagtt catcctgctg cagctcgcga gctgcggact gatctccgac
     7081 gacccggtgg tgtggcaatc gcagcgccac gacctctacc tgtcggccat gcggcgacag
     7141 ctgatgcggc accagcgctc gtttccctgc agctgctcgc gccgcgacat cgagcaggcg
     7201 tggctggccc acggccaggt gcgccgcgac cacggcgagc tggtctaccc gggcacctgc
     7261 cggcagggcc tgcacggcaa gcccgcccgg gcgcagcggc tgcgctgcac ccaggaagac
     7321 ggcagcgacc tggtgatcgc ctggaccgat cgacggctcg gtgcgatgac gcagaacgtg
     7381 acgcaggcca tcggcgactt cgtgctgcgg cgcgccgacg gcctctgggc ctatcaactt
     7441 gcggtggtgg tcgacgacgc cgaccagggc atcacgcacg tcgtgcgcgg gggcgacatg
     7501 gccgaaagca cgcctcgcca gatccacctc cagcatgagc tgggcgtgcc cacgccggcc
     7561 tacctgcaca ccccggtggt gatggccgca gacggccaca agctgtcgaa gcagaacggc
     7621 gccgaagcct tcgacccgcg cgagccgctg aaggccctgc gcgacgcggg cacggtgctc
     7681 ggcatcgagg tgcaagggga aacggtcgcc gactggctgg ccagcgcggt tccagcctgg
     7741 gcggcgttgt acccaccgac gctgccagag ccgccgacac agcaaggcgc accggaccgg
     7801 cctgcgccga tcggatgagg caggcggacg tcggcatcgc aagcaaaggg cgacgcggaa
     7861 gcgacccaag gcacatgctg gcttccgtct cggggaggtc agcgcgtggc gcggtcggaa
     7921 agttcacggc ctagggaggc cccatgcacg cagcatgcgg gcagacaacc tgtccagccg
     7981 ccagtgcttg acctcagcac cgtctgacgg cggccagcag tccggcccgc caagcccacc
     8041 ataatccgag catccacagg cacatcgtgc cgaaaagtga gtttgcatcc atgactccag
     8101 ccccaactgg ccgccgacag gccttggctt gggcggctgc gatgctggcg atgccggccg
     8161 tcgcgcagtc gccggccacg ctgaccatca gcaccaacaa caccccgctg gaccgcaagg
     8221 cgctggcgca attggccctg gaagcctgca gccggatcgg cgtgggcttc aagctggtga
     8281 gcctgccttc ggagcgctcg ctgcaggctg ccaaccaggg tgaggtcgat ggtgaaggcc
     8341 tgcgcgtggc cgggctggaa gaaacctacc ccaacctgct gcgcgtgcct gaacgcttca
     8401 tcggaatcag cttcgtggcc tttgccagca atcccgccat cacactcacc gaaggctggg
     8461 ccagcctgaa gccgcaccgc gtggccttca tcaatggctg gaagatgttc gagacccatg
     8521 ccacgggcgc gcgcgtggtg cacaagctgg acaaggccga acaactcttc cagatgctgg
     8581 ctgccgggcg catcgacctg gcgctctaca cccttgcgga tggcgtggcc ctggtgcggc
     8641 aactcggcct gtcaggcatc gcgccgctga cgcccgcgct gcgcgacgtg gacatgttcc
     8701 tctacctgca tcggagacac cagaacctgg tgctccgtct ggccgcggcc ctgcgcgaga
     8761 tgaagaccga cggcagccat caacgcatcc tcgcctccac aggtcccgat tgagtcccgg
     8821 caccgaaccc ggcccggcct cgctgcttcg tctgggcctg gccagccgcg tttcacggcg
     8881 catcctggcc tgggcgctgg cagtcggtgc cgtgggcacg ctggccgttt cctggtggga
     8941 tgccgagcgt gcctaccagg accagaccgc gcgcctgggc acctccttgc aaggtcttgc
     9001 ggagttcgca gcgccttcgc tggcggttgg cgcctggacc ttcgaccgca cgcagatcga
     9061 cacccagctc agcgccttca cccggatggc agacgtgagc gcagtcacgc tggccatcac
     9121 cggccagccg cctgtccggc tggccccgca cccgatctcc agcctggtgc tggagcgcaa
     9181 ggtgcccctg gtctacgtgg acgacgggcg cacccagacg ctgggcacgc tcacgctcac
     9241 gcacgacctg cagccgctgc aggctcaacg ccgctcgcaa tggctgcacg caatggccac
     9301 caatggcctg gtgatcctgc tgaccgccct ttcttcagtc ctgatctacc agttcatcgt
     9361 gacgaggcga ctgctggcca ttgcccacca gttgcgcggt gtcacggcgc aggacttgcg
     9421 ctcgctgccc cccgagcccc ccttgccggt ccggaaagcg cctgacgaac tggacgacct
     9481 ggcggcttcc atcgccacgc tgcaggccac gggccgccag gcgctgctgg ccggtgacga
     9541 cgagcacgcg ctcctgcgca gcctgatgga caccattccc gacctggtgt ggctgaagga
     9601 tgtgaacggc aagttcctgg cctgcaatcc gcgcttcgag caactcatcg gcgccggcga
     9661 gtcgcagatc ctgggcaagg acgactacgc cttcatgccc cgcgacgagg ccgatttctt
     9721 tcgtgacaac gaccgccgcg ccatcgaagc cggtggctcg tccgtcaacg aagaatggct
     9781 ggacttcaag gccgacggct accgaggctt gttcgagacc atcaagaccc cgatgcgcct
     9841 gcccgacggc cggctggtcg gggtgctggg catcgcccgc gaggtgacga agcagcgcgc
     9901 cgccgtcgag gccttgcgtg accgcgagga gctgtaccgc agcatcgtca gccgggctgc
     9961 agacggcatc ttgctggtgg accccgagac cggagccctt gaagagttca acgacgcagc
    10021 ctgcctgcaa ctgggctaca cgcgggaaga attcgcgcag ctcaacgtgt tcgaactgca
    10081 ggccgacttc agcccggcgg acgtgcggcg cagcttcgaa accacgcgca ccgagggcgg
    10141 cagccgattc gaacaccgcc accggcgcaa ggacggcagt ctgcgttcgg cctgggtcag
    10201 caccagtccg gtcacggtgc gcggtcgcac gctgatcacg gtgctgtggc acgacatctc
    10261 gagccgcatc gaggctgaag ccgcggtgca ggacgaacgg cgcatgaggg agacctttct
    10321 ggagtccatc ccgggcgtct tctacggtat cgacacctcc agtcgcctgg tgctctggaa
    10381 ccgcaatttc gaactcgcct tcgagcgcag tgcgcagcag ctcaccggtt tgccggccgc
    10441 ggacctgttt gaaggcgaag accgtctgcg catcgcgcag aacattgcca aggtctttgc
    10501 cgaaggccac accgaggtcg aggcggactt ccgaacgccg ggagggcgcc acgcggccta
    10561 cctgttcacc ggccggctgg tggagatcgg cggccagccg atgctggtcg gcgtgggtgt
    10621 ggacatctcg gcgcgccgcg aagccgaaca ggcgctcaag ggcctgaaca gccagcttga
    10681 acagcgggtg gtggcgcgca ccgccgactt gcgccgggcc cacgaacagt tgctcgacac
    10741 gcatttcgcg atggacagcg tgggcatcgg catcgcttgg gcggacagcg agaccgggcg
    10801 cttcgtctac gtcaaccgcc acatcgcgga ctttcttggc tactcggtgg acgaaatgct
    10861 gagcctgggc gtttccgaca tcgacccggc cttccccacg cacgactttc ccgcacatgc
    10921 ccaggccgtg cgcgaagcgg gccacctgca gttcgaaacc tcgcatcgca cacgcgacgg
    10981 acgcctgttg ccggtggaaa tgtcggtcta ctatcacgcg ggcaacagcc ggtccggacc
    11041 gcggatcatc gggttcatga ccgacatcgc gcggcgcaag gaattcgagc aggcgctgcg
    11101 gcggtccaag gaagaagccg aggccgccaa caaggccaag agcaccttcc tggccaacat
    11161 gagccacgag atccgcacgc cgatgaacgc catcatcggc ctcacgcatc tgatgcgccg
    11221 caacaacccg acacctcagc agctggattg gctggacaag atcgacgggt ccggccgcca
    11281 cctgctggcc atcatcaacg acgtgctgga cctggccaag atcgaggctg gccgcctgga
    11341 actcgaaagc accaacttcc acctttcgtc ggtgcttgac aacgtggtgt cgatcatccg
    11401 agagcctgca cgcgccaagg gcctggacat cgagatcgac accgccgatt cggtgccgat
    11461 gtggctcagc ggtgacccga cccggctgcg tcaggccttg ctcaactacg tcggcaacgc
    11521 cgtcaagttc accagacggg gctgcatccg cctgcgcacc gaactgctcg aagacgggcc
    11581 cgaggagctg ctggtgcgct ttgaggtgga tgacaccggc atcggcatcc cgcctgaaaa
    11641 actcgccggc ctgttcaccg aattcgagca ggccgacgcc tccaccacgc gccgccacgg
    11701 cggcacgggc ctggggctgg ccatcaccag ccggctggcg ctcctgatga atggcgaggt
    11761 cggcgcccgc agtgtgccgg gcgagggcag cagcttctgg ttcactgcgc ggctgcggcg
    11821 gggcctcgac gtggtgcctg gcagcgtggg agacaaaccg ccgcacgagg ctgagccctg
    11881 gatccaattg cagcgcatca atgcccgccg caccgcgcgc atcctgctgg tcgatgacaa
    11941 ccccatcaac cgcgaagtgg ccttgcagtt gctggacggc tccggcctgc tggtggacac
    12001 cgcggacaac ggtgccgagg ctgtgcgcat ggcggcggcc tgtccctacg acctggtgct
    12061 gatggacgtg cagatgccgg tgatggacgg cc
//
LOCUS       DAOORV010000106        12006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6593_length_12006_cov_7.103673, whole genome shotgun sequence.
ACCESSION   DAOORV010000106 DAOORV010000000
VERSION     DAOORV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_12006_cov_7.103673"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PKV56_13190"
     CDS             <1..159
                     /locus_tag="PKV56_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOX68813.1"
                     /translation="ARFANPFVAGARGFIDDVIQPHETRKRICRSLVMLRDKKLENPW
                     RKHGNIPL"
     gene            291..2321
                     /locus_tag="PKV56_13195"
     CDS             291..2321
                     /locus_tag="PKV56_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HOX68814.1"
                     /translation="MFTKILIANRGEIACRVIKTAKKMGIRTVAVYSDADKDARHVAL
                     ADEAVHIGPAPSRESYLVMDKIIAACKQTGAQAVHPGYGFLSENTEFARRVEEEGIVF
                     IGPKHASIAAMGDKIASKQLAGEARVNTIPGYNAAIDTAEHAVEIAQGIGYPVMIKAS
                     AGGGGKGLRVAYDDKQTFEGFTSCRNEARNSFGDDRVFIEKFVEEPRHIEIQVLGDAF
                     GNTVYLWERECSIQRRHQKVIEEAPSPFISEATRKAMGEQAVALAKAVNYQSAGTVEF
                     VVGKDQSFYFLEMNTRLQVEHPVTEGITGLDLVEMMIRVAAGERLFLTQEDIKRDGWA
                     IECRINAEDPFRGFLPSTGRLVKFAPPTETWTASGEVPEDGGVRVDTGVIDGGEIPMY
                     YDSMIAKLVVKGRDRLDAIAKMREALNAFAIRGISSNIPFQAALLAHPKFVAGDFNTG
                     FIAEQYPNGFSAAALPQTDPMLLRALAAVANRIHLDRSARITGQLPGHEFVIKAESVV
                     ITSDGEGRSDHVPVSVVPEGSGFRVELGGASYLICFDTPLREMVIHGSIDGRPFCAQI
                     ERIGLAYRVIHNGTQIDAKVVSPFAAQMAALMPFKAPPDLSRFLLSPMPGLLVDVTVK
                     PGQKVLAGEKLAVIEAMKMENILLATQDAVVAEVKAAKGESLAVDQVIIRFE"
     gene            2524..2988
                     /locus_tag="PKV56_13200"
     CDS             2524..2988
                     /locus_tag="PKV56_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOX68815.1"
                     /translation="MSTRPFKVLGIQQVAIGGPSKDRLRTLWVDKLGLEVTGNFVSER
                     ENVDEDICAMGSGAFKVEVDLMQPIDPEKKPAVHTTPLNHIGLWIDDLPAAVTWLTAN
                     GVRFAPGGIRKGAAGHDICFLHPKSNDEFPVAGEGVLIELVQAPPEIVAALG"
     gene            complement(3041..4141)
                     /locus_tag="PKV56_13205"
     CDS             complement(3041..4141)
                     /locus_tag="PKV56_13205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX68816.1"
                     /translation="MQRYPYIDILRGIAALLVIWYHVIENSGWTTFAYKGFSRLPRIG
                     WLGVDLFLVISGFVIAKAAIEAHATKANWQGYFAERRVRRILPLYLATLVCYVAMVNP
                     DVLNHEWDSFDHVASHLFLIHNLWPFTSGSINGPNWSVALEAQFYMLIVLVTPWLART
                     TWWRVLLTWVTIAVAWRYMTTLVWVPGLSDVNDQRVAATQLPGTLDQFGFGIALAKLA
                     ISGHLKFTPRRFAVALVIALLGLNAAWFIFWPRANYWYYPAMISLWRTLLSAGFAALL
                     ACVVMIPWKAGWFSWPFRYVGQISYGLYLWHIPVLMTLLERTPWKGYTLLCVTLVLTF
                     ILAALSWHGFEQLWMNPRKRAAPAATERDSPG"
     gene            4162..5859
                     /locus_tag="PKV56_13210"
     CDS             4162..5859
                     /locus_tag="PKV56_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68817.1"
                     /translation="MYIEFFGLKQEPFSIAPDPRYLFMSERHREALAHLLYGLGGGGG
                     FVLLTGEIGAGKTTVCRCFLEQIPENCNVAYIFNPKLTAIELVRSVCDEFGVAYARRE
                     PGLATVTDYLDPLNAFLLKAHAAGQNNVLIIDEAQNLSADVLEQLRLMTNLETRERKL
                     LQIILIGQPELRGMLAQPGLQQLAQRVIARFHLEALSESETAQYIRHRLSVAGRSGPL
                     PFADDTLRRIHRLCAGVPRRINLLCDRALLGAYASGQAVVDRRIVHKAAREVFDAAAR
                     SAGPTVPRRQMLLAALLAGLVGMVAAYAGARLWFDRSVPLQPPASAPSASSAASAPAA
                     ASAPSSAASAPSSALPASAPASAVPFDVNTSFSLLTRREGEAWRALASLWHVDAGPGA
                     GDPCPGMQAQQLPCYRTTTTLAVIRQLDRPGILTLRDPRDQPAYALLTGIGADAATLT
                     AGDVVMTVPLTVLANSWRGEFATFWRAPPTYRAPIQRGSSGPVVEALASRLATLSGEG
                     APVGLQEFDAGLHARVSAFQRAQGLQSDGIVGPTTFMQLNRASGVDEPRLLGDATGR"
     gene            5867..6634
                     /locus_tag="PKV56_13215"
     CDS             5867..6634
                     /locus_tag="PKV56_13215"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HOX68818.1"
                     /translation="MSYILDALKRAESERSRGAVPNIHVPHDAMAGRETTRHPAVRVG
                     IAAVVLLALLAAAWAWWFAPEPAGSSSARPGASVTDPVPTSAAQPIPRDVGPATTVAQ
                     PAGLPEPVAPVAVPVQSPMTPTRRPDVSVGVPTAAPAVARPQPRTVVPDARSATTVPA
                     VAASSARPETTAPGSEAGEERVYAVKELPDEIRGSLPTLTIGGATYSENASSRMLIVN
                     GALFHEGDKLTPEVTLQQIKLRTAVLSYRGYRYFISY"
     gene            complement(6656..8200)
                     /locus_tag="PKV56_13220"
                     /pseudo
     CDS             complement(6656..8200)
                     /locus_tag="PKV56_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004508.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(8328..9158)
                     /locus_tag="PKV56_13225"
     CDS             complement(8328..9158)
                     /locus_tag="PKV56_13225"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOX68819.1"
                     /translation="MMSDSRSPYEDLMRLVRDHGVFKPDRTGTGTKSVFGHQMRFDLT
                     AGFPLITTKKVHLKSVILELLWFLRGDGNARWLQERGVTIWDEWAKPDGDLGPVYGVQ
                     WRSWPTPDGGHIDQIADVIRTLKTNPDSRRIIVSAWNVADLDKMALMPCHAFFQFYVA
                     PSAVEGQPGRLSCQLYQRSADIFLGVPFNIASYALLTHMVAQQCDLQVGDFIWTGGDC
                     HLYSNHTDQVELQLSRTPRALPTLNIRRRPASIFDYEFEDFEVLGYEPHPAIKAPVAV
                     "
     gene            complement(9532..10194)
                     /locus_tag="PKV56_13230"
     CDS             complement(9532..10194)
                     /locus_tag="PKV56_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX68820.1"
                     /translation="MNSSYRSIHRPLWALAASALMLGGCANMTAEQQGTAQGAGIGAA
                     SGAVLGAVVGGRAGTGAVIGGVLGAVAGNIWSKRMEERKQAMEQATQGTGVEVSRTAD
                     NQLKLNIPNDISFDINSYAIKPQLRGVLDPFAASLREDPRARLMIVGHTDSTGNDAIN
                     NPLSVDRAQSVRDYLATRGVAVGRIETAGRGKNEPVADNATESGRAQNRRVEIFLREP
                     EV"
     gene            complement(10303..>12006)
                     /locus_tag="PKV56_13235"
     CDS             complement(10303..>12006)
                     /locus_tag="PKV56_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOX68821.1"
                     /translation="FLVMAAVSTLVFPVALKSLIDQGIVEADPGQRVMALREHFFALF
                     GVGAALGLFSALRFYMVTWLGERVTADLRNAVYAHVVKQSPEFFETTQTGEVLSRLTT
                     DTTLVQTVVGSSLSMGLRNAVMCVGAMTMLIVTNPVVMAQVLGILVLVVLPSVYFGRR
                     IRKLSRASQDRVADSSAIAAEVLNAIPVVQSYTQEAREAERFDVSTANAFATAVSRTR
                     MRSILVGFIITATFGALLWGLYQGTQAVVRGDITAGHLGQTVVYVIILVSGVAVLAEV
                     YGDLLRAAGATERLMELLATESPIRSPAIPRVLPEVQRGSSLSLDAVTFRYPSRPKQA
                     ALADFSLQVAPGETVALVGPSGAGKSTVFQLLLRFYDVAEGTVRIDGMPVRDLALEAL
                     RGRIGIVPQDSVIFSANAMENIRYGRPDASDAEVIAAAKAAFAHDFITALPDGYQTFL
                     GERGVRISGGQRQRISIARAMLKNPPLLLLDEATSALDAESERMVQAALESAMKDRTT
                     LVIAHRLSTVQRADRIVVMDGGRIVESGTHASLSASGGLYSRLAKLQFDAPDSLPAPT
                     A"
BASE COUNT         1966 a         3969 c         4008 g         2063 t
ORIGIN      
        1 gcccgcttcg ccaacccgtt cgtcgccggc gctcgcggct tcatcgatga cgtgatccag
       61 ccgcacgaaa cccgcaagcg catctgccgc tcgctggtga tgctgcgcga caagaagctc
      121 gagaacccgt ggcgcaagca cggcaatatt ccactttgac ggagcccccc agtcgctgtg
      181 ctcctgcccc cgaggggcgc cccgcactgg cccggccgag ccggatccac gcgggttgct
      241 ttgagtaggc ctcgcttcgc ttgcctgtgg caccaaaaag agagttgatg atgtttacaa
      301 aaattctgat tgcgaaccgt ggagagatcg cctgccgggt gatcaagacc gcgaagaaga
      361 tgggcatccg cacggtggcc gtctattccg atgccgacaa ggacgcgcgc cacgtcgcgc
      421 tcgccgacga ggcggtgcac atcgggccgg cgccatcgcg cgagtcgtac ctggtgatgg
      481 acaagatcat tgccgcctgc aagcagacgg gtgcgcaggc ggtgcatccc ggctacggct
      541 tcctcagcga gaacaccgag ttcgcgcgcc gcgtcgagga agagggcatc gtcttcatcg
      601 gcccgaagca cgcgtcgatc gcggcgatgg gcgacaagat cgcctcgaag cagttggccg
      661 gcgaggcccg ggtcaacacc atccccggct acaacgccgc gatcgacacc gccgagcacg
      721 ccgtcgagat cgcccagggc atcggctacc cggtgatgat caaggcatcg gccggtggcg
      781 gtggcaaggg cctgcgggtg gcctacgacg acaagcagac cttcgagggc ttcacctcgt
      841 gccgcaacga ggcgcgcaac agcttcggcg acgaccgggt cttcatcgag aagttcgtcg
      901 aggagccgcg gcacatcgag atccaggtgc tcggcgatgc tttcggcaac acggtttacc
      961 tgtgggagcg cgagtgctcg atccagcgcc gtcaccagaa ggtgatcgag gaagcgccgt
     1021 ccccgttcat cagcgaggcc acccgcaagg cgatgggtga acaggccgtg gcgctggcca
     1081 aggcggtgaa ctaccagagc gcaggcacgg tggagttcgt ggtcggcaag gaccagagct
     1141 tctatttcct ggagatgaac acccggctgc aggtcgagca tccggtgacc gagggcatca
     1201 ccgggctcga cctggtcgag atgatgatcc gcgtcgccgc tggcgagcgc ttgttcctga
     1261 cgcaggagga catcaagcgc gacggctggg cgatcgaatg ccgcatcaat gccgaggacc
     1321 cgttccgcgg gttcctgcca tcgaccggcc gactggtgaa gttcgcgccg cccaccgaga
     1381 cctggaccgc gagcggtgag gttccggaag atggtggcgt gcgcgtggat accggcgtga
     1441 tcgacggtgg cgagatcccg atgtactacg actcgatgat cgccaagctg gtggtcaagg
     1501 gccgcgaccg gctcgatgcg atcgcgaaga tgcgcgaggc gctgaacgcc ttcgcgatcc
     1561 ggggcatcag ttcgaacatc ccgttccagg cggcgctgct ggcgcacccc aagttcgtcg
     1621 ccggcgactt caacaccggc ttcattgccg agcagtatcc gaacggattc tcggccgccg
     1681 cgctgccgca gaccgatccg atgctgctgc gcgcactggc ggcggtggcc aaccgcatcc
     1741 acctcgaccg ttcggcgcgc atcaccggtc agctgccggg acacgagttc gtgatcaagg
     1801 ccgagtcggt cgtgatcacc agcgatggcg aaggccggtc cgatcacgtg ccggtgagtg
     1861 tggtgcccga gggcagcgga ttccgcgttg agcttggcgg tgcgagctac ctgatctgct
     1921 tcgacacgcc actgcgcgaa atggtgatcc acggcagcat cgacggtcgt ccgttctgcg
     1981 cccagatcga gcgcatcggc ctggcctacc gagtgatcca caacggcacg cagatcgatg
     2041 ccaaggtggt gtcgccgttt gccgcgcaga tggctgcgct gatgcctttc aaggcgccac
     2101 cggatctgtc gcgtttcctg ctttctccga tgccgggcct gctggtcgac gtgaccgtca
     2161 agcccggtca gaaggtgctg gcaggcgaga agctggcggt gatcgaggcg atgaagatgg
     2221 agaacatcct gctcgccacg caggatgccg tggtggccga ggtcaaggct gcgaagggcg
     2281 agagcctggc ggtggatcag gtgatcatcc ggtttgaata gggcccccca gtcgctgcgc
     2341 tcgcgccttg caggccgcgc tgggccagtg gtccagcccc tcacgaggcg caaccagttc
     2401 accggactgg ttgcgtccgg gcacggcccc cgaggggcgc cgcctggcgg cccggccaag
     2461 ccggttccgc gggcgtggct tgatgaggcg catcgcttcg cttgcgcgta aaggaacgtt
     2521 ttgatgagta cacgtccatt caaggttttg ggtatccagc aggtcgccat cggcgggccc
     2581 agcaaggatc ggttgcgcac gctgtgggtc gacaagctcg gcctcgaggt gaccggcaat
     2641 ttcgtcagtg aacgcgagaa cgtcgacgag gacatctgcg cgatgggtag cggcgccttc
     2701 aaggtcgagg tcgacctgat gcagcccatc gatccggaga agaagccggc ggtgcacacg
     2761 acgccgctga accacatcgg cttgtggatc gacgatctgc cggctgccgt cacctggctc
     2821 actgccaacg gtgtgcgctt cgcgccgggc ggcatccgca agggcgcggc aggtcacgac
     2881 atctgcttcc tccacccgaa gagcaacgac gagttcccgg tggcgggcga gggtgtgctg
     2941 atcgaactgg tgcaggcgcc gcctgagatc gtggcggcgc tgggctgatt tcagccagcg
     3001 gcccggactc ggcagaccgc tttgctccga ggccgaagcc tcagcccggt gagtcgcgct
     3061 cggtggccgc tggggccgcg cgctttctgg gattcatcca gagttgctcg aagccgtgcc
     3121 acgacagggc tgcgaggatg aacgtcagca cgagggtgac gcacagcagg gtgtatccct
     3181 tccacggggt tctctcgagc aaggtcatca gcacggggat gtgccagagg tacaagccgt
     3241 agctgatttg tccgacatag cggaaaggcc atgaaaacca gcctgccttc cacggaatca
     3301 tcaccacgca ggcgagcaac gctgcgaagc ctgcagacag cagggtgcgc cacaggctga
     3361 tcatcgcggg gtagtaccag tagttcgcgc gtggccagaa gatgaaccag gctgcgttga
     3421 gccccagcag cgcgatcacg agggccaccg cgaagcgccg cggcgtgaat ttcagatgcc
     3481 ccgagatggc cagcttggcc agggcgatgc cgaagccgaa ctggtcgagc gttccgggca
     3541 gctgcgttgc cgcgacccgc tgatcgttca cgtcgctcag gcccggcacc cagaccagcg
     3601 tcgtcatgta tcgccaggcg accgcaatcg tgacccacgt cagcagcaca cgccaccacg
     3661 tggtgcgcgc cagccacggc gtcaccagga cgatgagcat gtagaactgg gcttccagcg
     3721 ccaccgacca gttgggcccg ttgatcgatc ccgaggtgaa cggccacagg ttgtggatca
     3781 ggaacaggtg cgatgcgacg tgatcgaacg agtcccattc atggttgagc acatcgggat
     3841 tcaccatggc gacgtagcag acgagcgtgg ccaggtacag cggaaggatc cggcgcacgc
     3901 gtcgctcggc aaagtagcct tgccagttcg ctttcgtggc gtgcgcctcg atggcggcct
     3961 tggcgatcac gaagccgctg atcaccagga acagatcgac gccgagccag ccgatgcgtg
     4021 gcagacgcga gaagcccttg taggcgaagg tcgtccaccc gctgttctcg atgacgtgat
     4081 accagatcac gagcagcgcg gcgatgccgc gcaggatgtc gatgtacgga tagcgctgca
     4141 taggccgcta ttctctccac catgtacatc gaattcttcg ggctgaaaca ggagccgttc
     4201 tcgatcgctc cggatccccg ctatctgttc atgagcgagc ggcaccgcga ggcgctggct
     4261 cacctgctgt acggactggg tggcgggggc ggcttcgtgc tgctgaccgg cgagatcggc
     4321 gctggcaaga ccacggtgtg ccgctgcttc ctcgagcaga tccccgagaa ctgcaacgtc
     4381 gcctacatct tcaacccgaa gctcaccgcc atcgagctgg tgcgctcggt gtgcgacgag
     4441 ttcggcgtcg cctacgcccg gcgcgagccg ggcctggcga ccgtcaccga ctacctcgat
     4501 ccgctcaatg ccttccttct gaaggcgcat gcggcgggtc agaacaacgt gctgatcatc
     4561 gacgaggccc agaacctgtc ggccgatgtg ctcgagcagc tgcggctgat gaccaacctg
     4621 gaaacccgcg agcgcaagct gctgcagatc atcctgatcg gccagcccga gctgcgcggc
     4681 atgctggcgc agccgggcct gcagcagctg gcgcagcgcg tgatcgcacg cttccacctg
     4741 gaggccctga gcgagtccga gacggcgcag tacatccgcc accggctctc ggtggccggc
     4801 cggtccggac cgcttccgtt tgccgacgac accttgcggc gcatccatcg cctgtgtgcc
     4861 ggcgtgcctc gtcgcatcaa cctcctgtgc gaccgcgcgc tcctgggcgc gtatgcgagc
     4921 ggccaggcgg tggtcgatcg gcgcatcgtc cacaaggcgg cgcgcgaggt gttcgatgcc
     4981 gcggcacggt cggccgggcc gaccgtgccg cggcgccaga tgctgctcgc ggcgctgctg
     5041 gcgggcctcg tgggcatggt cgcggcgtat gccggggcca ggctctggtt cgacaggtcc
     5101 gtgccgctgc agccgccggc gagcgcgcct tcggcttcct cggcggcttc ggcgccagct
     5161 gccgcttcag cgccttcctc cgcggcgtcc gcgccatcga gtgcgctgcc cgcctcggcg
     5221 cctgccagcg cggtgccctt cgatgtgaac acctctttca gcctgctgac gcgtcgcgag
     5281 ggtgaggcct ggcgtgcgct ggcctcgctc tggcacgtcg acgcgggccc gggagccggc
     5341 gatccgtgcc cgggcatgca ggcgcagcag ttgccctgct accgcacgac gacgacgctg
     5401 gcggtgatcc ggcagctgga tcgcccgggc atcctgacgc tgcgggatcc ccgcgaccag
     5461 cccgcctacg cgctgctcac gggcatcgga gcggatgcgg ccacgctgac tgccggcgat
     5521 gtggtgatga ccgtaccgct gaccgttctg gccaactcct ggcgcggcga attcgcgacc
     5581 ttctggcgcg ctccgccgac ctatcgcgcg ccgatccagc gcggaagttc cggtcccgtg
     5641 gtcgaagcgc tggcatcccg gctcgcaacg ctgtcaggcg agggcgctcc ggtcggcctg
     5701 caggaattcg acgcgggcct gcacgcccgg gtgtccgcat tccagcgagc ccagggcctt
     5761 cagtccgacg gcatcgtcgg gcccaccacc ttcatgcaac tgaaccgggc cagcggggtc
     5821 gacgagcctc gcctgctcgg tgacgcgacg ggccgctgaa ccgaacatgt cgtacatcct
     5881 cgatgcgctc aagcgcgcgg aatccgaacg cagccgcggt gcggttccga acattcacgt
     5941 accgcacgac gcgatggccg ggcgggaaac gacccgtcac ccagccgtgc gcgtcgggat
     6001 cgccgcggtc gtgctgctgg ccttgctggc tgccgcctgg gcgtggtggt ttgcgccgga
     6061 gcctgccggc tcgtcatcag ccaggccagg cgcctctgtc accgatcccg tgccgacatc
     6121 ggcggcgcag ccgatccctc gtgacgtggg gcctgccacc acggtggcac agcccgctgg
     6181 cctgcccgag cccgttgcac cggttgctgt tccggttcag tcaccgatga cgccgacgcg
     6241 ccggccggac gtctctgtgg gcgtcccgac tgcggcgcct gctgtggcaa ggccgcagcc
     6301 gcgcacggtg gtgcctgacg ctcgcagcgc caccacggtg cctgcggtgg ccgcttcgtc
     6361 ggcccggccc gagacgactg cgccaggctc ggaggctggc gaagagcgcg tctatgccgt
     6421 gaaggaactg cccgacgaga ttcgaggcag cctgccgacg ttgaccatcg gcggggcgac
     6481 gtattcggag aacgcgtcca gccgcatgct gatcgtcaac ggtgccctct tccacgaggg
     6541 cgacaagctg acgcccgagg tgacgctgca gcagatcaag ctgcgaaccg cggtgctgag
     6601 ctaccgcgga taccggtact tcatcagcta ctgaagaatc gcggtcacgg cggggtcagc
     6661 gaaacacgct gaccgcctcc accagacgct cggcctgcag cttcaggctg tccgaggccg
     6721 ccgcgctttg ttcgaccagc gccgcgttct gctgggtgat ctcgtccagg cctccgatgg
     6781 cctgcccgat ctgggaaatg ccgctttcct gctcctgcgt ggccgaggaa atctcggcga
     6841 tcaggtcgga gacgcgcttc acctcgctga cgatgtcgcc catcgtcttg ccggcgtcat
     6901 cgaccagctt ggttcctgct tcgacgcggt cgacgctcgc gctgatcagc gccttgatct
     6961 cccgggctgc atcggcactg cgctgcgcca ggctgcgcac ctcgcccgcc accaccgcga
     7021 agccgcggcc ctgctcgccg gcgcgtgcgg cttcgacggc agcattcagc gccaggatgt
     7081 tggtctggaa ggcaattccg tcgatcacgc cgatgatgtc ggcgatcttg cgcgagctct
     7141 gattgatctc gttcatggtg tcgaccacct gggacacggc atgcccaccc ttggcggctg
     7201 cctggctggc cgagcctgaa agctggttgg cctggccggc cgtgtcggca ttgttcttga
     7261 ctgcggctgt catctgcatc atcgacgagg cggtctgctg cactcgtgct gccgcctgct
     7321 cggtgcgtgt gctcaggtcg ttgttgccct gcgcgatctc ggtggtcgcg accttcatgt
     7381 tcagcacctg ctcgctgacg tcatcgatca gccagcggaa catcaggccg agctggccga
     7441 cggcgcgcag cgtcatgccg atctcgtcga ctcggtttcc gtggtccacc cgctggcttt
     7501 ctcccgaggc gactttcagg gcctgcgcat acagggcctc gagcgggcgc gagatctgtg
     7561 cctccagcgc ccacgatgcc agcagcaagg cggctgccgc cacgccaccg aggccggcga
     7621 gggccgttcc accgatcccg agcgccacgg caccggccat cagcgcgggc cagaccgcgc
     7681 agagcgcgcc acgaatgcgc cagcgcaccg gcatggtctg gagcatcgaa gcccatcggc
     7741 gcagacccgt gcgcacgacg actcccttgt gaagcttgcg gtcgcccgcc ttgccttcgc
     7801 gaaaatctcg gtacagggct tctgcacctg cgacctcgtc gcgggagggc ttggttcgca
     7861 cggacatgta gcccgcgagc ttgccgtggc ggaccaccgg catcgcgttg gcgcgcactc
     7921 agtagtggtc gccgttcttg cggcggttct tcacgatgcc ggtccacggc tccccgcctt
     7981 tcagcgtcgc ccacatgtcg gcgaaggcct ccctgggcat gtcggggtga cgaaccacgt
     8041 tgtgaggctg gccctggatt tcctcgcgcg agaagccgct gatttcaatg aaagcggcgt
     8101 tcccgtacat gacgcggctg tccaggtcgg tggtcgacat cagcgtggcg cggtcgggca
     8161 cctcatattc ctgtccggtg accggctggt tgttgcgcat gcggggttcc tcctggctga
     8221 gccgatacgc tgtccggacc ggatcggtcg gacagccgtt gcccattcca tggaatcggc
     8281 tgcaaaggcc ggaaacttga gccctctcga acccgggggg gcgtggccta gacagccacc
     8341 ggcgccttga tggccggatg aggctcgtag ccgagcacct cgaaatcctc gaactcgtag
     8401 tcgaagatcg acgccggccg ccgcctgatg ttcagcgtgg gcaacgcacg gggtgtgcgc
     8461 gacagctgca gctcgacctg atcggtgtgg ttgctgtaga ggtggcagtc accgccggtc
     8521 cagatgaagt cgcccacctg cagatcgcat tgctgcgcca ccatgtgcgt cagcagtgcg
     8581 tagctcgcga tgttgaacgg caccccgagg aagatgtcgg cgctgcgctg gtagagctgg
     8641 cagctgagcc gcccgggctg gccttcgaca gccgagggcg ccacgtagaa ctggaagaac
     8701 gcgtggcagg gcatcagcgc catcttgtcg aggtcggcca cgttccacgc gctgacgatg
     8761 atgcggcgcg aatcgggatt ggtcttcagt gtcctgatga cgtcggcgat ctggtcgatg
     8821 tgaccgccat ccggagtcgg ccagctgcgc cactgcacgc cgtagaccgg tccgaggtcg
     8881 ccgtcgggct ttgcccattc gtcccagatc gtcacgccgc gctcctgcag ccagcgcgca
     8941 ttgccatcgc cgcgcaggaa ccacagcaat tcgaggatca ccgatttcag gtgcaccttc
     9001 ttcgtcgtga tcagcgggaa gcccgccgtc aggtcgaagc gcatctggtg tccgaagacg
     9061 cttttcgtgc cggtgccggt gcggtccggc ttgaacacgc cgtggtcgcg caccaggcgc
     9121 atcaggtctt cgtagggcga tcgactgtcg gacatcattt gaaaaccgtg cttgttcgcg
     9181 cgattatcaa agctgcagac tcgcttaccg tcgttttgtc gctgagcggt aaacgctgtg
     9241 caatgcgatg gctgcgatca gcaacagaag aagtagtaat gcgccgagcg gatgctgatc
     9301 caaggtcacg atcaacgttg tcaagtctac gttggctatg gaaatcatga gtgtgctccg
     9361 atgcggtcca ccgccgcttc tgaacatcac tatgccatct caccgcatat gcccttcggc
     9421 ttccagacca gttgtcaaca ggttgtcaac tggtcttttt ggtatgactt ggccttgcgt
     9481 gaagtgcgaa tcaaagcgtg gacattgcac aactgtgcga caggtcaaag atcacacttc
     9541 cggttcgcgc aggaatatct cgacgcggcg gttctgtgct cggccggatt cggtggcgtt
     9601 gtccgcgacg ggctcgttct tgccgcggcc ggcggtttcg atccggccga cagccacgcc
     9661 gcgggtcgcc aggtagtcgc gcacgctctg cgcccggtcc accgacagcg ggttgttgat
     9721 cgcgtcgttg ccggtgctgt cggtgtggcc gacgatcatc aggcgggcgc gcgggtcttc
     9781 gcgcaggctg gccgcgaacg ggtcgagcac accgcgcaac tgcggtttga tcgcgtagct
     9841 gttgatgtcg aaggagatgt cgttcgggat gttgagcttg agctggttgt cggccgtgcg
     9901 gctgacctcg acgccggtgc cctgcgtcgc ctgctccatc gcctgcttgc gctcttccat
     9961 gcgcttggac cagatgttcc cggccacagc cccgagcacg ccgccgatga ctgcgccggt
    10021 gccggcccgc ccgcccacca cggcgccgag caccgcgccg ctggcggcgc cgatgccggc
    10081 gccctgtgcc gtgccttgct gctcggcggt catgttggcg caaccgccca gcatcaaggc
    10141 gctggcggcc agggcccaca agggccgatg gatcgatctg tagctgctgt tcatgaagac
    10201 ctctggatca gggatcgctg cccggtgcag cgtggtctga tcgtgcttca tcgcagcgca
    10261 ttcggctgtc ggaatgccaa cagcttgcgt gtcagaaggg acctacgcgg tgggggcggg
    10321 cagggaatcc ggtgcatcga actgcagctt cgccagcctc gaatacaggc cccccgaggc
    10381 gctcaggctc gcgtgcgttc cgctctcgac gatgcgcccc ccgtccatca ccacgatgcg
    10441 atcggcgcgc tgcaccgtcg acaaccggtg ggcgatgacc agcgtcgtcc ggtccttcat
    10501 cgccgattcg agcgcggcct gcaccatccg ctcgctctcg gcgtccagtg cgctggtggc
    10561 ttcgtcgagc agcagcagcg gagggttctt cagcatcgcg cgtgcgatgc tgatgcgctg
    10621 gcgctggccg cccgagatgc gcacgccacg ttctccgagg aaggtctggt agccgtcggg
    10681 cagcgcggtg atgaagtcgt gcgcaaaggc agccttcgcg gccgcgatca cttcggcatc
    10741 actggcgtcc ggccggccgt agcggatgtt ctccatcgcg ttggccgaga agatgacgct
    10801 gtcctgcggg acgatgccga tgcgcccgcg cagcgcttcg agcgccaggt cgcgcaccgg
    10861 catcccgtcg atgcgcaccg tgccctcggc cacgtcgtag aagcgcagca gcaactggaa
    10921 taccgtgctc ttgcccgcac cgctcgggcc gaccagcgcg acggtttcgc cgggcgccac
    10981 ctgcaggctg aaatcggcaa gcgcggcctg cttcggccgc gacgggtagc ggaaggtcac
    11041 ggcgtccagt gacagcgacg aaccgcgctg cacctctggc agcacccgcg ggatcgcggg
    11101 cgagcggatc ggcgattcgg tggccagcaa ctccatcagc cgctcggtcg caccagcggc
    11161 gcgcagcagg tcgccgtaga cctcggcgag caccgccacg ccgctgacca ggatgatcac
    11221 gtagaccacc gtctgtccca ggtggccggc ggtgatgtcg ccgcgcacca ccgcctgcgt
    11281 gccttgatac agaccccaga gcaacgctcc gaaggtggcg gtgatgatga agccgaccag
    11341 gatcgaacgc atgcgcgtgc ggctgaccgc ggtggcgaag gcgttcgcgg tcgacacgtc
    11401 gaagcgctcg gcctcgcggg cttcctgcgt gtagctctgc accacgggaa tcgcgttcag
    11461 gacctcggca gcaatggcgc tggaatcggc cacgcggtcc tggctggcac ggctgagctt
    11521 gcggatgcgg cggccgaaat agaccgacgg cagcaccacc agcacgagga tgccgagcac
    11581 ctgtgccatc accaccgggt tggtgacgat cagcatcgtc atcgcaccga cacacatcac
    11641 cgcgttgcgc agacccatgc tcagcgagga gccgacgacg gtctgcacca gcgtcgtgtc
    11701 ggtggtcagc cgcgacagga cctcgccggt ctgagtggtc tcgaagaatt cggggctctg
    11761 cttgacgacg tgggcgtaga ccgcgttgcg caggtcggcg gtgacgcgtt cgccgagcca
    11821 ggtgaccatg tagaagcgca gtgccgaaaa caggcccagc gctgcaccga cgccgaacag
    11881 cgcgaagaag tgctcgcgca gagccatcac ccgctggccc ggatcggcct ccacgatgcc
    11941 ctggtcgatc agcgacttca gcgccaccgg gaacacgagt gtgctgaccg ccgccatcac
    12001 caggaa
//
LOCUS       DAOORV010000107        11931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6651_length_11931_cov_7.422701, whole genome shotgun sequence.
ACCESSION   DAOORV010000107 DAOORV010000000
VERSION     DAOORV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6651_length_11931_cov_7.422701"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2695
                     /locus_tag="PKV56_13240"
     CDS             <1..2695
                     /locus_tag="PKV56_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOX68822.1"
                     /translation="TLHLTAAQPITMQARLLQPQMRDSRGTPARPVGWDHALDHLADR
                     FAAIIASHGPDAVGFYLSGQLLTEDYYVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYK
                     ATLGADSPPACYDDLNHADTLFIAGSNAAFAHPILFRRIEDAKRARPGMRLIVVDPRR
                     TETAEAADLFLQILPGTDVALFNGMLNLMLWEGLTDGAHIAAHTSGFDALRQRVRDYA
                     PRDVARLCGIAETDLVQAARWFAGLDGAQVSVRRPTLSLYCQGLNQSSSGTAKNAALI
                     NLHLACGQIGRPGAGPFSLTGQPNAMGGREVGGLATALSAHRDLANPGHRAEVAALWG
                     VADVPGAPGKSAVEMFEAAADGQIKALWIACTNPAQSMPDQATVRRALERCELVVVQE
                     AYATTATARYADVLLPATSWGEKDGTVTNSERCISRVRPAVSAPGEARHDWAIGVDFA
                     RRLESRLHGDRRAAVRLPSASSTLFAYDCPRSVWNEHRESTRGRDLDITGLDYARLDR
                     SPAQWPCPEGATAGRSRLYEDGRYATPDGRARFVDTAYEPLAEPRDARYPYSLNTGRL
                     RDQWHGMSRTGTLGRLFGHEPEPGVDMHPRDMARLGWVNGDLIQLDSRRGGLILPIRG
                     CEQVAPMQCFIAMHWGDEHVWGAARDGRRAAGVNALTSSAYCPESKQPELKHAAVKLA
                     KVDLPWRLLALAWLPADEVLIVRQRLADLVEDLGSALPFALCVPFGRERTGLLLRAAA
                     PGPLPRAWLEELEAVLGLGRAGVLHYADDKRGQRRSMRLAAHADGGQRLDALLLGGDI
                     SAEAWVKTVLQEDLPAQAYGRLLLSPGAQPPVAVAARGRQVCSCFDVSEPRIVDALRA
                     CVGSGEERLLQLQQGLKCGTQCGSCVPELKRLERATAPQPA"
     gene            2724..3539
                     /gene="cobA"
                     /locus_tag="PKV56_13245"
     CDS             2724..3539
                     /gene="cobA"
                     /locus_tag="PKV56_13245"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOX68823.1"
                     /translation="MNTVEAATRTSRGSVTLVGAGPGDPELLTLKAVRAIRRATLLLV
                     DELVGDGVLRYARSSARIVHVGKRGGCKSTPQTFIQQLLLREAQAGERVVRLKGGDPF
                     VFGRGGEEAEFLRAHGVDVEVVNGITSGLAAATALQVPLTHRDHAHGVILVTGHSRGN
                     DAALHWPTLAAAAAQGLTLVIYMSVATAADVQAGLLQALPASTPVAVVQSASLPQQRH
                     AVGSLGGLVALIARHGLGSPAIILVGDVLAGLRQVGGMEQLDEGRRGTGVRVA"
     gene            3640..5172
                     /locus_tag="PKV56_13250"
     CDS             3640..5172
                     /locus_tag="PKV56_13250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOX68824.1"
                     /translation="MTALYRTILLCVSAWLGCTAVHAAEPMAPEVMAALRESGVPLKS
                     FGFYAQPVEQVPQGSGPVLASMNEEQTFLLASTTKVVTSLAALDLLGPAHSWPLRAYA
                     TRPVVGGQLRGDLVIAGNRHTITAAELRQWFQQMRAEGLQSIQGQLVLEGVLLVPEDD
                     AADTAAALESGDARRAQAERAGPDAAAHDDGSMMLSIEPGPGDRARVTLTPKPVGVVV
                     VNEVLMGGTCDAWARWSPAGDNAEGVPHLWVRGRWDANCPKEYVAYVRPLPGMKYAPD
                     VVLPAADRASAPRHAGSLARTVAALWAESGGTLRGRVVESDFRPLQPGAAAAKATWSS
                     VYSIPLAQVVREINKTSDNLAARSLLLSLSASQASENQARQSAKNRVHEWLRAQGLID
                     GDIRVDLGSGQSREERGKPRAMVRLLCNAWRAEGAQAFVNSLPIAGVDGTLVNRMRNG
                     LATGQAFLKTGTLSDTRALAGYVRSRSGVIYAVTAIVNHPDAARARPALDVFIGWVVK
                     HG"
     gene            5323..6486
                     /locus_tag="PKV56_13255"
     CDS             5323..6486
                     /locus_tag="PKV56_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009882 - blue light photoreceptor
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOX68825.1"
                     /translation="MSTTSASMFGGRGGRLATGSLIAPPRLATLVYRSRCVEPWSAQA
                     LRRMVDAAQLRNRSESITGLLICEEDRFFQWLEGPSDRLARVWDSIRQDPRHTDIEIL
                     GDAPSPVRFFGDWDMKLATRQTLNPEAVRLAAAGLAASGAGAVVDFPYDRPWSRSDER
                     LDPLLTEHPRVGELARLLFAADPDLASLLIDELHLGTDSMPQLFATVFEPAARCLGDL
                     WMQDECCEFDVTLGLGRLQGAVRSLSSSTSQRVLSASRLPSVLVAPVPGERHTLGATL
                     DAEVLWQAGWDMRCELPATDAALGKLLASQWFDALDLSLSAAFEREHWLPRLARTIAF
                     ARASSRNPALAVVVGGRAFFERGDRHAQVGADAACPTALRIDPLLFGMVGTTV"
     gene            6578..6811
                     /locus_tag="PKV56_13260"
     CDS             6578..6811
                     /locus_tag="PKV56_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68826.1"
                     /translation="MLLGIVGLLVTLAIVALIARTQLRQVTATAPAVSSEESSASAAE
                     GGEPTATRDLPGKIQQDVTRAMQQAPQRDDDSR"
     gene            6825..7424
                     /gene="tadA"
                     /locus_tag="PKV56_13265"
     CDS             6825..7424
                     /gene="tadA"
                     /locus_tag="PKV56_13265"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOX68827.1"
                     /translation="MTAPISPQDEAAMRIALDQAHNAWLVGEVPVGAVILRAGQVIAT
                     GYNRPITEHDPTAHAEIVALRHAATLLGNYRLPECELYVTLEPCAMCAMALLHARFKR
                     VVFGAPDPKTGAAGSVIDLFAQSQLNHHTQVQGGVMAEACSAVLRDFFAERREQYRQR
                     RSGVTANVDAGGLPSRPEGDEAVIPAGEALELPSPTDTI"
     gene            7432..8409
                     /locus_tag="PKV56_13270"
     CDS             7432..8409
                     /locus_tag="PKV56_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOX68828.1"
                     /translation="MTSVLLYSPSGIVAAAPLRRAARRLTALGYEVEIDASALAKHQR
                     FAGDDDTRLAALHRVAERAPDIALATRGGYGLTRLLDRIDWPLIHRSIERGTRWVGHS
                     DLTVLQLGLLAHRDASSKGAGTFAARGDDRDSAHHHADHGAVGLTWAGPMACGDFGGE
                     VVDDITEPCFVEAMSGELEAVGFRTEAGFDGLDARGTLWGGNLCMVTSLLGTAYWPKP
                     LTTRGGILFLEDVNEHPYRIERSLLQLQQAGVLDAQKAVLLGDFSGYRRSPLDRGYQL
                     KTAIAYLRSVTKTPILTGLPFGHVPTKVTLPVGARVRLLVQGRDVLVGW"
     gene            8409..10622
                     /locus_tag="PKV56_13275"
     CDS             8409..10622
                     /locus_tag="PKV56_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX68829.1"
                     /translation="MGRVAWAILVAIAILLAGCNNNPLPDGAGASNTLFTAVSGSSPR
                     HLDPTASYWSNETPYTYQIYEPPYGYHYLKRPFVLQGKTAVEVAQPTYLDHAGHKLPA
                     DAPAADIAESVYEVRIKPGILFQPHPAFATDAQGRHRYHSEIALKAGEIGDRRSPWEF
                     RHQGTRELVAEDYVYAIKRHATTRITTPIFGIFSEYVLGLKEYGELIKAEDAKLRAGL
                     DPAALDKPFLDFRQWPLIGATAPGKHLLRIRIKGKYPQWNYWMALTFMSPVPWEADAF
                     YAQPGMAANGLSLDHWPVGTGPYMMAESVQDTRHVMKRNPNYRGEPYPCEGVEADRTN
                     GLLADCGRKTPFIDTLVSVIEKEGVPLRAKFRQGYYDIPRIERTDDGLDFLVEMDDSE
                     DVRRDYTERGFRLPKFADVTSWYLGFNMLDPVIGQGDTPERQAKNRRLRQAISIAIDW
                     EEYSRIFPKSAGETAMGPLPDGVFGSRHGQPQGVNAVTHRVVDGKQVRRPIADARQLL
                     AEAGYPDGRDAVTGRPLVLNYDYYSQPTPEVKSQLDWMVKQFAKIDVQLEIRATDNNQ
                     FQDKVRQGKHQIFWSGWLADYPDAENFLFLLYGPNAKSKSDGENTANYANPEYDRLFA
                     QLKGVDDGPEKQALIDRMTAIVQQDAPWSFGYFPYSSGAFQSWVHNAKPAVLVRDMAR
                     YYRIDAAERVRRQTDWNRPVWWPMLIGVAALVAVVVLAVRSFRRRERLDARGAVL"
     gene            10639..11622
                     /locus_tag="PKV56_13280"
     CDS             10639..11622
                     /locus_tag="PKV56_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68830.1"
                     /translation="MLSTIVRRIAYGVLILIGVNLATFFLFFTVNTPDDMARLNIGGK
                     RVTQEQIVKWKVERGYDKPLYLNPAREGAARYTDTIFWERSVSLFTLRFGRADAESAG
                     DIGHQVATRMVVSLQLALPLFILQVIASTAFALLLVMFRQTRFDAWGVVLCVLMLSIS
                     SLFYIIVGQFLFSRVLRLAPISGYAPGLDAVKFLVLPIALSLLARLGGEARLYRAMFL
                     EETGKDYVRTARAKGLGEPRVLYAHVLRNALIPIVTSAGSYLPYVFLGSLVFESFFGL
                     PGLGAYVIDAISNQDFAVVRTMVFLGSLLYVLSYIVIDIAYTWIDPRVRLT"
BASE COUNT         1849 a         4010 c         4121 g         1951 t
ORIGIN      
        1 cacgttgcat ctgacggccg cgcagccgat cacgatgcag gcgcgcttgc ttcagccgca
       61 gatgcgcgac agtcgcggca ccccggcgcg gcccgtcggc tgggaccacg cactcgacca
      121 cctggccgac cgattcgcgg ccatcattgc cagtcacggg cccgatgccg tcgggttcta
      181 cctgtccggt cagttgctga ccgaggacta ctacgtcttc aacaagctgg ccaaggggtt
      241 gatcggcacc aacaacgtcg acaccaactc ccgcctgtgc atgagcagcg cggtggctgg
      301 ctacaaggca acgctcgggg ccgattcgcc gccggcctgc tacgacgacc tcaatcacgc
      361 cgacacgctt ttcattgccg gctccaacgc agccttcgcg cacccaatcc tgttccggcg
      421 catcgaggac gcgaagcgcg cgcgtcccgg aatgcgcctg atcgtggtcg atccgcggcg
      481 caccgagact gccgaggcgg ccgacctgtt cctgcagatc ctgcctggca ccgacgtggc
      541 cctcttcaac ggcatgctga acctgatgct gtgggagggc ctgaccgacg gcgcgcacat
      601 cgccgcgcac accagcggat tcgatgcgct gcgccagcgc gtgcgcgact acgcgccccg
      661 cgacgtggcc aggctgtgcg gcatcgccga gaccgatctg gtgcaggcgg cccgctggtt
      721 cgccgggctc gatggcgcgc aggtatccgt gcgccgcccg acgctgtcgc tctactgcca
      781 gggcctgaac cagagttcga gcggaaccgc caagaacgct gcgctgatca acctgcacct
      841 ggcctgtggg cagatcggca ggccgggggc cgggccgttc tcgctgaccg gacagcccaa
      901 cgcgatgggc ggacgggagg ttggtggcct ggcgaccgcg ctcagcgctc accgcgatct
      961 ggccaacccc gggcaccgtg ccgaagttgc tgcactgtgg ggtgtcgcag acgtgcctgg
     1021 cgcccccgga aaaagcgccg tcgagatgtt cgaggcggct gccgacggcc agatcaaggc
     1081 cttgtggatc gcctgtacca atccagcgca gtcgatgccc gaccaggcca ccgtgcgacg
     1141 cgcgctcgaa cgctgcgagc tcgtggtcgt gcaggaggcc tacgccacca ccgccacagc
     1201 gcggtacgcc gatgtgctgc tgccggcgac ttcctgggga gagaaggacg gcaccgtcac
     1261 caacagcgag cgctgcatca gccgggtgcg acctgccgtt tcggcgcccg gcgaagcacg
     1321 tcacgactgg gctatcggcg tggacttcgc gcgccggctc gagagccgac tccatggcga
     1381 ccggcgggca gcggtccggt tgccgtcggc gtcgagcacc ctgttcgcgt acgactgccc
     1441 gcgatcggtc tggaacgagc atcgtgaatc cacgcgggga cgcgatctgg acatcaccgg
     1501 gctcgattac gcgcgcctgg accggtctcc tgcgcagtgg ccttgcccgg aaggcgccac
     1561 cgccggtcga tcgcgcctct acgaggacgg ccggtatgcc actccggacg gcagggctcg
     1621 cttcgtcgat accgcctacg agccgcttgc cgaaccgcgc gacgcgcgtt atccgtattc
     1681 gctcaacacc gggcgcctgc gcgaccagtg gcatggcatg agccgcaccg gcacgctcgg
     1741 ccgcctgttc gggcacgagc ccgagcccgg ggtggacatg caccctcggg acatggcgcg
     1801 tctcggctgg gtgaacggtg acctgatcca gctggactcg cggcgcgggg gattgatcct
     1861 gccgatccgc ggctgcgagc aggtggcacc gatgcaatgc ttcatcgcga tgcactgggg
     1921 ggacgaacac gtgtggggtg cagcacgcga tgggcgtcgc gcggccggcg tcaatgcgct
     1981 gacctcgtcc gcgtactgcc ccgagtccaa gcagccggag ctgaagcacg cggcggtcaa
     2041 gctggccaag gtggatctgc cttggcggct gctcgcgctg gcctggttgc ctgcggacga
     2101 ggtgctgatc gtgcggcaga gactggccga cctggtcgag gacttgggga gcgccttgcc
     2161 cttcgcgctg tgcgtgcctt tcggacgcga gcgaacgggc ctgttgttgc gcgcagcggc
     2221 gccgggaccc ttgcctcgag cctggctcga ggagctcgag gccgtgctgg gcctggggcg
     2281 ggccggcgtg ctgcactatg ccgacgacaa gcgcgggcag cggcgcagca tgcggctggc
     2341 ggctcacgcc gatggcggtc agcgcctgga cgccctgctg ctgggcggag acatcagcgc
     2401 ggaggcctgg gtgaagaccg tgctgcagga ggatctgccc gcgcaagcct acggacgcct
     2461 gctgctgtcg cccggggccc aaccgccggt ggctgtcgcg gcccggggac gccaggtctg
     2521 cagctgcttc gatgtcagcg agccccggat cgtcgatgcg ctgcgcgctt gcgtcggttc
     2581 cggggaagaa cggctcctgc agttgcagca agggctcaag tgtggaacgc agtgcggttc
     2641 atgcgtgccc gagttgaaac ggctcgagcg cgcgaccgcg ccgcagccgg catgacgacc
     2701 acgaattcag gagaaggaac gcgatgaaca ccgtagaagc cgccactcgc accagccggg
     2761 gcagcgtcac gctggtcggg gccggcccgg gcgaccccga gctgctgacg ctcaaggcgg
     2821 tgcgggcgat ccgcagggcc acgttgctgc tggtcgacga actggtcggt gacggggtgc
     2881 tgcgctacgc cagaagctcg gcgcgcatcg ttcacgtcgg caagcgtggt ggctgcaaga
     2941 gcacgccgca gaccttcata cagcagctgc tgctgcgcga ggcgcaggcc ggcgagcgcg
     3001 tggtgcggct caagggtggc gacccgttcg tgttcgggcg cggcggcgag gaagccgagt
     3061 tcctgcgcgc ccacggtgtc gacgtcgagg tcgtcaacgg catcacgtcg gggctggccg
     3121 cggccacggc gctgcaggtg ccactgacgc atcgtgacca tgcacacggt gtgatcctgg
     3181 tcaccgggca ttcgcgtggc aacgacgccg cgctgcattg gcccacgctg gcggctgcgg
     3241 cggcgcaagg tttgacgctg gtgatctaca tgagtgtggc gaccgccgcc gacgtgcagg
     3301 cggggctgct gcaggcgctg cccgccagca cgccggtggc cgtggtgcag agcgccagcc
     3361 tgccgcagca gcgccacgcg gtgggctcac tggggggtct ggtggcgttg atagcgcgtc
     3421 acggtctggg cagtccggcc atcatcctgg tcggcgatgt gctcgcgggc ctgcgacagg
     3481 tcggggggat ggaacagctc gacgagggtc gccgcgggac tggcgtgcgg gtggcctgac
     3541 ctcatgccgc gcccggtcgg gtgcggaatg tcgcaggatc gggctgcgcg cttgattgcg
     3601 gtgagccagg accgggtggc atcggagtac gctcggagga tgactgccct gtaccgaacc
     3661 atcctcctgt gtgtctctgc ctggctgggc tgcactgcag tgcatgcggc cgagccgatg
     3721 gcgcctgaag tcatggcggc gctgcgcgaa tccggcgtgc cgttgaagag cttcggcttc
     3781 tatgcccagc cggtcgagca ggtgccgcag ggctccggcc cggtgctggc ttcgatgaac
     3841 gaggagcaga cgttcctgct ggcgtcgacc acgaaagtgg tgacttcgct ggctgccctg
     3901 gatctgctcg gaccggccca cagctggccg ctgcgcgcct acgccacccg cccggtcgtg
     3961 ggtggccagt tgcgtggcga cctggtcatt gccggcaacc ggcacaccat caccgccgcc
     4021 gaattgagac agtggttcca gcagatgcgc gccgaaggcc tgcagtccat ccagggccag
     4081 ctggtgctcg aaggcgtgct gctggtcccc gaagacgatg cggccgacac ggctgccgcg
     4141 ctggagtcgg gcgacgcccg gcgggcccag gcggaacgcg ccgggcccga cgcggcggcc
     4201 catgacgacg gctcgatgat gctgtccatc gaacccgggc cgggtgatcg ggcccgcgtc
     4261 acattgactc cgaagccggt cggcgtggtg gtcgtcaatg aggtgctgat gggcggaacc
     4321 tgcgatgcct gggcgcgctg gagccccgcc ggcgacaacg ccgaaggcgt tccgcacctg
     4381 tgggtgcgcg gacgctggga tgccaactgc ccgaaggagt acgtcgccta cgtgcgcccg
     4441 ttgcccggca tgaaatacgc tcccgacgtg gtgcttcccg ccgcggatcg tgcttcggcg
     4501 ccgcgccacg ccggttcgct tgcgcgcacc gtcgcggcac tgtgggctga atcgggtggc
     4561 accctgcgcg gtcgcgtggt cgagagcgac tttcggccac tgcagcccgg tgctgccgcg
     4621 gccaaggcaa cctggtccag cgtctactcg atcccactgg cgcaggtggt ccgcgagatc
     4681 aacaagacca gtgacaacct cgctgcgcgc agcctgctgc tgtcgttgtc ggccagtcag
     4741 gcctcggaga accaggcgcg gcagagcgcg aagaaccgcg tgcacgaatg gctgcgcgcc
     4801 caggggctga tcgacggcga catccgggtc gacctcggct cgggccagtc gcgtgaggaa
     4861 cgcggcaagc cacgcgcgat ggtgcggctg ctgtgcaacg cctggcgggc cgaaggcgcg
     4921 caggcgttcg tcaattcgct gccgatcgcc ggcgtggacg gcacgctggt caaccgcatg
     4981 cgcaacggcc tggcgaccgg gcaggccttc ctgaagaccg gcacgctgag cgacacccga
     5041 gcgctcgccg gatacgtgcg cagccgcagc ggagtgatct acgccgtcac cgcgatcgtc
     5101 aatcacccgg acgccgcgcg cgccagaccg gcgctcgacg tgttcatcgg ctgggtggtc
     5161 aagcacggat agggaaagct cccggacttc gggatcgcat ccgaggttca tgcgcgttgt
     5221 caggcttgca agttacagaa tgtgtcacct agacttggcg ctcagtctgg taagtgcctg
     5281 agcggttttc cgccagcgaa tgccggagac gaggagcgcg tgatgtccac cacgagtgcg
     5341 tcgatgttcg gggggcgggg cggtcgcctt gcgactggtt cgctgatcgc ccctcctcga
     5401 ctggccacgc tcgtgtaccg cagccgttgc gtcgaaccct ggtcggcgca ggcacttcgt
     5461 cgcatggtgg atgccgcgca gctgcgcaat cgctcggagt ccatcaccgg tctgctgatc
     5521 tgcgaggaag acaggttctt ccagtggctg gaaggtccat ccgacaggct ggcccgcgtc
     5581 tgggactcga tacggcagga cccgcggcac accgatatcg aaatcctcgg tgatgcccct
     5641 tccccggtgc ggttcttcgg cgactgggac atgaagctgg cgacccggca gaccctgaat
     5701 ccggaagcag tgcgtcttgc ggcggccgga ctggcggcat ccggtgcggg tgctgtcgtc
     5761 gactttccgt acgacaggcc atggtcccgg tccgacgagc gtctggaccc cttgctgacg
     5821 gagcatccac gggtcggtga actggcccgc ttgctcttcg ctgcggatcc cgacctggct
     5881 tcgctgctga tcgatgaact gcacctgggt accgattcca tgccgcagct cttcgccacc
     5941 gtgttcgagc cggccgcacg atgcctcggc gacctgtgga tgcaggacga gtgctgcgag
     6001 ttcgacgtca cgctgggtct gggtcgattg cagggcgcgg tccgcagcct gagcagttcg
     6061 accagccagc gtgtgctgtc cgcttcccgg ttgccctcgg tgctggtagc tccggtgccg
     6121 ggcgagcgtc acacgctggg agccacgctg gatgccgagg tcctctggca ggccggatgg
     6181 gacatgcgct gcgagttgcc cgcgaccgac gctgccctgg gcaagctgct ggccagccag
     6241 tggttcgatg cgctcgatct gtctctcagc gcggcattcg agcgcgaaca ctggctgccc
     6301 cgactcgcac gcaccatcgc tttcgcacgt gcgtcgtccc gcaaccctgc gctcgcggtg
     6361 gtggtcgggg gtcgggcctt cttcgagcgg ggcgatcgcc acgcacaggt cggtgccgac
     6421 gcggcctgtc ccaccgcgct gaggatcgac ccgctgctgt tcgggatggt cggaacgacc
     6481 gtttgaccgg gttcgtgcgt ggcggtggcc tgcacccgct agcatccacc gctggcgacg
     6541 gcgccccgat gccgtcagac gggaaacacg aatgcggatg ctgctgggta tcgtcggttt
     6601 gctggtcacg ttggcgatcg tcgcgctgat tgccaggact cagttgcggc aggtgactgc
     6661 cacagcgcct gcagtctcct cggaggaatc gtccgcatcg gctgccgaag gcggcgagcc
     6721 gacggccacc cgcgatctgc ccggcaagat ccagcaggac gtcacccgcg cgatgcagca
     6781 ggcgccgcag cgtgacgacg attcccgttg acaccattgc acgcatgacg gcaccgatct
     6841 cgccgcagga cgaagcggcc atgcgcatcg ctctcgacca ggcccacaac gcctggctgg
     6901 tcggcgaggt gccggtcggt gcggtgatcc tgcgggccgg ccaggtgatc gccaccggct
     6961 acaaccgtcc catcaccgag cacgacccca ccgcgcacgc cgagatcgtc gcgttgcgtc
     7021 acgccgccac cctgctcgga aactaccgtc tccccgagtg cgagctctac gtcacgctcg
     7081 agccttgcgc gatgtgcgcg atggcgctgt tgcacgcccg cttcaagcgc gtggtgttcg
     7141 gtgctccgga tcccaagacc ggtgccgcag gttcggtgat cgacctgttc gcccagtcgc
     7201 agttgaacca ccacacgcag gtgcagggtg gcgtgatggc cgaagcctgc agcgcggtgc
     7261 tgcgcgactt ctttgccgag cggcgcgagc agtatcggca gcggcgatcc ggcgtcaccg
     7321 cgaatgtcga cgcgggtggc ctgccgagcc ggccggaagg cgacgaagcc gtgatcccgg
     7381 caggcgaggc cctcgaactg ccgtctccca ccgacacgat ctgagccttc catgacctcc
     7441 gttcttctct acagcccatc gggcatcgtc gctgcggcgc cgctgcgacg cgccgcacgg
     7501 cgcctgaccg cgctgggcta cgaggtcgag atcgatgcct cggcgctggc gaagcaccag
     7561 cgttttgctg gcgacgatga cacgcgtctg gcggccctgc atcgggttgc cgagcgcgcg
     7621 cccgacatcg cgctcgccac gcgcggcggc tacggtctga cccgcctgct ggaccgcatc
     7681 gactggccgt tgatccaccg cagcatcgag cgcggcacgc gctgggtcgg ccacagcgac
     7741 ttgacagtgc tgcaactcgg cctgctggcg caccgcgacg cttcgagcaa gggagccggc
     7801 accttcgccg cccggggcga cgatcgcgat tcagcccatc accacgccga ccacggcgcg
     7861 gtcggattga cctgggccgg accgatggcc tgcggcgact tcggcggcga ggtggtcgac
     7921 gacatcaccg agccgtgctt cgtggaggcg atgagcggcg agctcgaagc ggtgggcttc
     7981 cgcaccgaag ccggcttcga cggactcgac gcccgcggca cgctgtgggg tggcaacctg
     8041 tgcatggtca cttcgctgct cggcaccgca tactggccga agccgctcac cacccggggc
     8101 ggcatcctgt tcctcgagga cgtcaacgag catccgtacc gcatcgagcg cagcctgctg
     8161 cagctgcagc aggcgggcgt gctcgatgcg cagaaggcgg tgctgctcgg cgacttcagc
     8221 ggttaccgcc gatcaccgct cgatcgcggc taccagttga agaccgccat cgcctacctg
     8281 cgcagcgtca cgaagacccc catcctgacg ggcctgcctt tcggccacgt gccgaccaag
     8341 gtcacgctgc ccgtgggcgc cagggtgcgg ctgctggtgc aggggcgcga cgtgctcgtc
     8401 ggctggtgat ggggcgggtc gcctgggcga tcctggtcgc gattgcgatc ctgctcgccg
     8461 gctgcaacaa caacccgctg cccgatggcg ccggcgcgtc caacacgctg ttcaccgcgg
     8521 tgtcgggcag ctcgccgcgc cacctcgacc cgacggcctc ctactggagc aacgagacgc
     8581 cctacaccta ccagatctac gagccgccct atggatatca ctacctgaag cggccgttcg
     8641 tgctgcaagg caagaccgcc gtcgaggtgg cccagcccac gtacctcgac cacgcggggc
     8701 acaagctgcc tgccgatgcc ccggcggccg acatcgccga gagcgtctat gaggtgcgca
     8761 tcaagccggg catcctgttc cagccgcatc cggcgttcgc gaccgatgca cagggtcgcc
     8821 accggtatca cagcgagatt gcgctgaagg cgggcgagat cggcgaccgc cgctcgccct
     8881 gggagttcag gcaccagggc acccgcgagc tggtcgccga ggactacgtc tacgcgatca
     8941 agcgccacgc gaccacgcgc atcacgacgc cgatcttcgg catcttctcg gagtacgtgc
     9001 tggggctgaa ggagtacggc gaactgatca aggcggagga cgcgaagctg cgtgccggcc
     9061 tcgacccggc ggcgctcgac aagccgttcc tcgatttccg ccaatggccg ctgatcggcg
     9121 ccactgcacc cgggaagcac ctgctgcgca tccgcatcaa gggcaagtac ccgcaatgga
     9181 actactggat ggcgctcacg ttcatgtcgc cggtgccgtg ggaggccgat gcgttctatg
     9241 cgcagccggg catggcggcc aacgggctgt cgctcgacca ctggccggtc ggcaccgggc
     9301 cctacatgat ggccgagtcg gtgcaggaca cccggcatgt gatgaagcgc aacccgaact
     9361 accggggcga gccctatccc tgcgagggcg tcgaggccga ccggacgaac gggctgcttg
     9421 ccgattgcgg ccggaagacg ccgttcatcg acacgctggt gtcggtgatc gaaaaggagg
     9481 gcgtgccgct gcgcgccaag ttccgtcagg gctactacga catcccgcgc atcgagcgca
     9541 ccgacgacgg gctggacttc ctggtcgaga tggacgactc ggaggacgtt cgccgcgatt
     9601 acaccgagcg gggttttcgc ctgccgaagt tcgccgacgt gacgtcttgg tacctcggat
     9661 tcaacatgct cgaccccgtg atcgggcagg gcgacacgcc cgagcgacag gcgaaaaacc
     9721 gcaggttgcg gcaggcgata tcgatcgcga tcgactggga ggagtacagc cgcatcttcc
     9781 cgaagagcgc tggcgagacc gcgatgggac cgctccccga tggcgtgttc ggatcgcgcc
     9841 acggccagcc gcaaggcgtg aacgcggtga cgcaccgtgt cgtcgatggc aagcaggttc
     9901 gccgcccgat agccgatgcc cggcaactgc tggcagaggc gggctacccc gatgggcgcg
     9961 acgcggtcac cggccgtccg ctggtgctca actacgacta ctacagccag cccacgcccg
    10021 aggtgaagtc gcagctcgac tggatggtca agcagttcgc caagatcgat gtgcagctcg
    10081 agatccgcgc gaccgacaac aaccagttcc aggacaaggt gcgccagggc aagcaccaga
    10141 tcttctggtc gggctggctg gcggactacc cagatgccga gaatttcctg ttcctgctgt
    10201 acgggccgaa cgccaagtcg aagtccgatg gcgagaacac ggcgaactac gcgaacccgg
    10261 aatacgaccg cctgttcgcg cagctcaagg gcgtcgatga cgggcccgag aagcaggcgc
    10321 tgatcgaccg catgaccgcc atcgtccagc aggatgcacc gtggtccttc ggctactttc
    10381 cgtactcgtc cggggccttc cagtcctggg tgcacaacgc caagcccgcc gtgctggtgc
    10441 gcgacatggc tcgctactac cgcatcgacg cggccgagcg ggtgcgccgc cagaccgact
    10501 ggaaccggcc ggtgtggtgg ccgatgctga taggcgttgc agcccttgtc gcggtcgtgg
    10561 tgctggccgt gcgcagcttc cggcggcggg aacggcttga tgcccgggga gcggtactgt
    10621 gatgacgcga ggcctgccgt gctgagcacc atcgtccgcc gaatcgccta tggcgtgctg
    10681 atactcatcg gcgtcaacct ggcaaccttc ttcctgttct tcaccgtcaa cacgccggac
    10741 gacatggcgc ggctgaatat cggcggcaag cgcgtcacgc aggagcagat cgtcaagtgg
    10801 aaggtcgagc gcggctacga caagccgctg tacctgaacc cggcgcgcga aggcgcggcg
    10861 cgctacaccg acacgatctt ctgggagcgc tcggtgtcgc tgttcacgct ccggttcggg
    10921 cgggccgatg cggaaagcgc cggcgacatc ggccaccagg ttgccacgcg catggtggtc
    10981 agcctgcagc tcgcgctgcc gctgttcatc ctgcaggtga tcgcgagcac cgcgttcgcg
    11041 ctgctgctgg tgatgttcag gcagacacgc ttcgatgcct ggggcgtcgt gctgtgcgtg
    11101 ctgatgctgt cgatctccag cttgttctac atcatcgtcg ggcagttcct gttctcgcgc
    11161 gtgctgcggc tggcaccgat ctcgggctac gcgccgggtc tcgatgccgt caagttcctg
    11221 gtgctgccga tcgcgctgtc gctgcttgcc cggctgggcg gcgaggcccg gctgtatcgc
    11281 gcgatgttcc tcgaggaaac cggcaaggac tacgtgcgca ccgcgcgagc gaagggactc
    11341 ggcgagccgc gcgtgctgta cgcgcacgtg ctgcgcaacg cgctgatccc gatcgtcacc
    11401 agcgcgggca gctacctgcc ttatgtcttc ctgggcagcc tggtgttcga gagcttcttc
    11461 gggctgccgg gtctgggcgc gtatgtcatc gacgcgatca gcaaccagga tttcgctgtc
    11521 gtgcgcacga tggtgttcct cggctcgctg ctctacgtgc tgagctacat cgtgatcgac
    11581 atcgcctaca cctggatcga tccgcgggtg cgattgacat gatggtgtcc acacgctcat
    11641 ggcactcgct gcccccgatg gggagtgtca gtgtcttcgg gcggccggtt ggcctgagga
    11701 tggaaatcaa ttcttcaact ccttgcctgc ttgatgcgga gcgcgatcac gccgacggtg
    11761 tgcactgctc cgcgaatgtc ccccggcgtc ggcctgaagg ccgggcctcc tcctttattt
    11821 cgctgcgcag cgcacaccgc cggcgtgatc cgccagcacc gtggctgatc cacagataca
    11881 agacacaaga cgccgtcacc cgtgccgggg acgctgggta tctcccgcag c
//
LOCUS       DAOORV010000108        11908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6672_length_11908_cov_8.395090, whole genome shotgun sequence.
ACCESSION   DAOORV010000108 DAOORV010000000
VERSION     DAOORV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6672_length_11908_cov_8.395090"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKV56_13285"
     CDS             <1..678
                     /locus_tag="PKV56_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX68831.1"
                     /translation="ACPYDLVLMDVQMPVMDGLEATRQIRGLPDWDRTPIVAMTANAF
                     AEDRLACEMAGMSDFVPKPVEPADLYATLLKWLRDPELPPAPRQHAEAPATPEPVVRP
                     VATLWERLLAVPGLDVELGVQRLLGRRERYLDILQRCVSAQCAPEAILRQAMQAGDRA
                     TAQLEAHGLKGAASTLCAHDLAARAARLEAALRDCSEPVGDDAAVQQAWSELQQTLDR
                     LDAILAG"
     gene            complement(730..2157)
                     /locus_tag="PKV56_13290"
     CDS             complement(730..2157)
                     /locus_tag="PKV56_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68832.1"
                     /translation="MKTLTLRIYLTVVAVLLLFAVASGWLFQQQIETERSRAETVLSE
                     RMAAWGDLIQRSLPGDEAPPEQQASALLDWSQRLRLPLALDDAAGKRIAMSESFAERL
                     QQPPDDAPPRAGTVAGRTGERSDRNRAYAVQLEDGRTLWVMRPGRRIGPGMGPPQPGG
                     PRGGPGPLPLVPPLYPLSFVPAPWQGGAGLVLLLGLLFVAVSLGAWPVVNRLTRRLQG
                     LRQGVEQFGAGELSHRVDATGSDEVAAVAASFNQAASRIEALVRSHQSLLANASHELR
                     SPLARMRMAVSMLDGATGAQMDRLKREIELDVAELDALVEEVLLASRLDALRDIERRD
                     AIDLLGLVAEEAARVGVAVDGRPTSVSGDERLLRRAVRNLIENAQRYGGGEVKIALTA
                     DTQGIAVHVCDRGPGVPADLRERIFEPFYRLPGHAETAGGVGLGLSLVKQIAERHGGR
                     VRCEARDGGGSCFVLSLPLHQAAAG"
     gene            complement(2188..2919)
                     /locus_tag="PKV56_13295"
     CDS             complement(2188..2919)
                     /locus_tag="PKV56_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX68833.1"
                     /translation="MAARLLMIDDDTRLSAMVGDYLRAAGFEVEVAGTLAEGRDRLAA
                     AGGAPLHAPGFDALVLDLMLPDGDGLDLCRELRAEARTRHLPLLMLTARGEPMDRIVG
                     LELGADDYLPKPFEPRELLARVKALLRRAAPVNAQAEEVLRFGRLEVDLAARVARLDG
                     RPCDLTSHQFELLVVLAQSPGRVLSRDQIMDSLKGHPMEAFDRSIDVHISRIRALIED
                     DPKEPRRVLTVRGAGYVFAKKQDAD"
     gene            complement(2997..3614)
                     /locus_tag="PKV56_13300"
     CDS             complement(2997..3614)
                     /locus_tag="PKV56_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOX68834.1"
                     /translation="MAQAKTGRFHFAPLQGAAGRWALGAVIALSATLGVAAWAHDPAH
                     GDRRGHHAEMGGRGGFGPGLFAGPAQRVERAVDRLLDGLDATDAQRTQIKQIAAAAAA
                     DLKTQHEAARSLHERSVQLFTAPVVDANAAEQLRQQMLAQHEQVSRRTLQAMLDASRV
                     LSPEQRATLAQRMADHRAHGGRPHADKASSGGRHGERAEPARLLS"
     gene            complement(3775..6624)
                     /locus_tag="PKV56_13305"
     CDS             complement(3775..6624)
                     /locus_tag="PKV56_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX68835.1"
                     /translation="MTEPGTTRSQVLLADRRRKLQMLAAQPDRVFELFVRSALQLTQS
                     DVAAVSLVGEEWQWFKAGVGLEAVHIPLSLSFCAHGIETGTTFEVVDAAADVRFAQHP
                     LVNRDGGLRSYAGHPIEFEGEQLGMLFVMSAQPRTLTPEQMRWLQDLAQGAADAAASH
                     ERIFKLQISQRRLLDFASASSDWLWETDSSHHYTWLSDRFEDLTGLTVQSQLGQNPPA
                     SPMRDGAGRVVEPLLGFLEVLDRQQSFARVVVLKPTPLGDRLISYSAIAKFGSDGMFR
                     GYRGSAQDVTHRIGAERQEREVSDTLRLLAQHVPGVLYQRVSRPDGTGYCPYASESVR
                     QLFEITPEKLALDSGPMFDRIHPEDRKSVEAQIRSAENSGQPWSQTFRVVLPEAGLRV
                     LSGFASPRRMPDGNVLWHGFTTDVTESEKAAAEIARSGQQWELASQAAGIGIFELNLS
                     TATITLDRNACQVHHLPAESPTAFVLAQWLDMLEPSARESAQAFISLAAVSGVIQREV
                     LGVVTTSGEPRRIEFVARAQASRDRTRSRTPVLIGVCRDITEEYAIERLRQEMAETRE
                     ANRSKSELLSRISHELRTPLNGILGFAQLLEGDRKEPLTSIQKQRVQTIRMSGTRLLS
                     LVNDVLELSRIEQQMFSLQRESVSVNSVVNSGIALLQPLALERALQVEFIGAEHELHV
                     KADRRAMDQVFTNLLSNAIKYNRAGGQATVIVEPLGTSKVQISIEDTGVGMSEAQVDQ
                     LFQPFNRVGAEKTQIEGTGLGLVIARELTQGMGGTIEVTSTPHTGSCFTIKLPLAKGS
                     QTGRDSTDSMPAQTDWIEAAGGTALYIEDDPVNAMLMREVFGRLGNWKLLEAATGAEG
                     LRLALAEQPHLLLTDMNLPDMSGLDIVKQIRQQPATRRMQVIAVTADALPQQQEMARQ
                     IGVDGYWTKPLDLALILKHLRGFFSANPDFPLSTK"
     gene            complement(6873..7058)
                     /locus_tag="PKV56_13310"
     CDS             complement(6873..7058)
                     /locus_tag="PKV56_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HOX68836.1"
                     /translation="MNKDQVKGRVEQAKGAAKEVAGKVTGNKELEIEGRIDKTAGKVQ
                     SGFGDAKEKLKDRIDKV"
     gene            complement(7245..7625)
                     /locus_tag="PKV56_13315"
     CDS             complement(7245..7625)
                     /locus_tag="PKV56_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOX68837.1"
                     /translation="MSEPVDHLPPASITVACLCAEWCGTCREYRTVFEQAVQGRRDAG
                     VRGLWIDVEDESDLVGAVDVDNFPTLLMARGGEVLFFGTITPHASTLARLVQAALDGQ
                     THLGGEPPDADVRALAARLQQVAG"
     gene            complement(7695..8057)
                     /locus_tag="PKV56_13320"
     CDS             complement(7695..8057)
                     /locus_tag="PKV56_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HOX68838.1"
                     /translation="MKNRSSTSGLVYSTDSGRMCPACRQPVAQCRCGQATAPVATDGI
                     VRVSRETQGRKGKGVTVIRGVVLDAAALAQLGRDLKAACGSGGTVKDGVIEIQGDHRD
                     RIVERLEAAGMKVKRAGG"
     gene            8181..8999
                     /locus_tag="PKV56_13325"
     CDS             8181..8999
                     /locus_tag="PKV56_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOX68839.1"
                     /translation="MALLILSTATLLLLTWLLREPLFTEWRRRKVRRQPFPAEWRQVL
                     RHRVPYFRSMPADLQLQLKKHIQVFLSEKPFIGCRGLVVTDEMRVTIAAQACLLILNR
                     RCDYFSQLRQILLYPGAFVVDRVHADGSGVVQDRRLALSGESWSLGQVILSWEDAVEG
                     AEVADDGRNVVIHEFAHQLDQESGAANGSPRMPGRARRERWARVLSEEFAVLQRRASE
                     RRPSLLSDYGATDPAEFFAVASEVFFEQPQRLAAEHAALYRELSHYYRVNPLSW"
     gene            9727..11808
                     /locus_tag="PKV56_13330"
     CDS             9727..11808
                     /locus_tag="PKV56_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX68840.1"
                     /translation="MKKSRNSLSAARRRRITSPTSLLAAAAALSGIIPLDVASAEPAT
                     RDSTLTLGSVTVTGRSSGPLPVRSVLSSVDILGEDLLRNQQVNNSWELFGRAPGVLLT
                     PFKQGNVSGKFSFRAFNGEGELNAVKLLIDGIPSNANDGSMQFLDMISPLEIQRLEIV
                     RGTNDARYGLHNIAGNANVVTRQGGNETLASVGIGSFNTREGQLAHGIEGDNWSQNYF
                     IGYRKSGNYRDHADSDKLTLSGKWFYTTDDSRFRAGLIARHYHNNADEPGYLTRADAR
                     DHPRQSYALSSTDGGKRDMDALSAHLDVRLTDEVSWSAKAYGNELKDKRYIRFSASAS
                     QQERVTDETQYGALTSLSWRPKVTWAREFSLEGGADINREHNNSLRYLTAERARQSQT
                     RDQSFSFDTQGAYVQAVIAPIESLKVIPAYRIDDVEGSFTDRLTRRTSPINDFGLIKQ
                     PKVSVVYAPWQQGSVYGNWGRTFQVGAGSAAYKIPPRTADLKPSINEGWEAGVKLQPT
                     DWIDGRIAVWEQKASDEVRRKLNDPAGDSENVGKTRRRGLDLQVNLRPEKRINAWFAY
                     SAQRSRIIEPDPAAPTTRGKQIDHVPRHLLSGGVDVQALPELRLWASANGQSSYFLER
                     TNSTGKFGSYLLLNLGVSYQLTRSISVDAQLKNVADRYTEYAWWDGAQSLHSPGDGRA
                     FYLTLNWKLDR"
BASE COUNT         1953 a         4134 c         3915 g         1906 t
ORIGIN      
        1 gcctgtccct acgacctggt gctgatggac gtgcagatgc cggtgatgga cggcctggag
       61 gccacgcgcc agattcgcgg cctgcccgac tgggaccgga cgccgatcgt ggcaatgacg
      121 gccaacgcgt ttgccgagga ccggctggcc tgcgagatgg ccggcatgag cgacttcgtg
      181 cccaaacccg tggaaccagc cgacctgtac gccacgctgc tcaagtggct gcgcgacccg
      241 gagctccctc ccgcgccgcg ccagcacgcc gaggcgcccg ccacccccga gcctgtggtc
      301 aggccggtgg ccacgctgtg ggagcgcttg ctggccgtgc cggggctgga tgtcgaactg
      361 ggcgtgcagc gcctgctggg ccgacgtgaa cgctacctgg acatactgca gcgctgcgtc
      421 tccgcccagt gtgcgcccga agccatcttg cggcaggcga tgcaggcggg tgaccgcgcc
      481 acggcccagc tggaggcgca tggcctgaag ggcgcggcat caacgctgtg cgcgcacgat
      541 ctggcggccc gggctgcccg gttggaagcg gccctgcgcg actgcagcga gcctgtcggc
      601 gacgacgcgg ccgtgcagca ggcctggagc gagctccagc agacgctgga tcggctggac
      661 gcgatcctcg cgggctaggg ccggctcggc ggaccccgcc ggcaaggctg caacccgcag
      721 tcccgaaggt cagccggcag cggcctgatg cagaggcagg ctcaacacga aacagctgcc
      781 cccaccgtcg cgcgcttcgc agcgcacgcg gcccccgtgt cgctcggcga tctgcttgac
      841 gaggctcagg cccagcccga caccacccgc tgtctcggcg tggcctggca gccgatagaa
      901 gggctcgaag atgcgctcgc gcaggtctgc gggaacaccg ggcccgcgat cgcagacatg
      961 caccgcgatg ccttgcgtgt cggcggtcag ggcgatcttg acctcgccgc ctccgtaccg
     1021 ttgcgcgttc tcgatgaggt tgcgcaccgc gcggcgcagc aggcgctcgt cgcccgacac
     1081 gctggtcggc cggccgtcga cggcgacacc gactcgggca gcctcttcgg ccacgagccc
     1141 gagcagatcg atcgcgtctc gccgttcgat atcgcgcaac gcatcgaggc ggctggccag
     1201 cagcacctcc tcgaccagcg cgtcaagctc ggcgacatcg agctcgatct cgcgcttgag
     1261 gcggtccatc tgtgcgccgg tcgcgccatc gagcatcgac accgccatgc gcatgcgcgc
     1321 cagtggcgag cgcagctcgt ggctggcatt ggcaagcagc gactggtgcg agcgcaccag
     1381 cgcctcgatg cgcgacgccg cctggttgaa gctggccgcc accgccgcga cctcgtcgct
     1441 gccggtcgcg tcgacgcggt ggctcagttc accggcgccg aactgctcga ccccctgccg
     1501 caagccctga agccgccggg tcaggcggtt caccacaggc caggcgccca gggacaccgc
     1561 cacgaacagc agaccgagca gcagcaccag gcccgcgccg ccctgccacg gcgccggaac
     1621 gaaggacagc gggtacaagg ggggcacgag cggcagcggg cccggcccgc cacgcggtcc
     1681 gccaggctgg ggcgggccca tgcccgggcc gatccgacgc ccgggtcgca tcacccacaa
     1741 cgtgcggccg tcctccagct gcaccgcgta ggcgcgattg cgatcgctgc gctcgccggt
     1801 gcgccccgca acggttccag cacgcggcgg agcgtcgtcg ggcggctgct gcaggcgctc
     1861 ggcaaacgac tcggacatcg cgatgcgctt gccggctgca tcgtcgagcg cgagcggcag
     1921 gcgcaggcgt tgcgaccagt cgagcagcgc ggaagcctgc tgctcgggcg gtgcctcgtc
     1981 gcccggaagc gagcgctgga tcaggtcgcc ccaggcggcc atgcgctcgg acaacaccgt
     2041 ctcggcacgg ctgcgctcgg tctcgatctg ctgctggaac agccagcccg acgccacggc
     2101 gaacagcagc agcacggcca ccaccgtcag gtagatgcgc agcgtcagcg tcttcatgcg
     2161 cgggtcgcca cccgggcgac gacagactca gtcggcatcc tgcttcttcg caaacacgta
     2221 gcccgcacct cgcacggtca gcacgcgccg aggctccttc ggatcgtcct cgatcagcgc
     2281 gcggatgcgc gagatgtgca cgtcgatcga gcggtcgaag gcctccatcg ggtggccttt
     2341 cagggaatcc atgatctggt cgcgcgacag cacgcggccg ggactctgcg ccagcacgac
     2401 cagcaactcg aactggtggc tggtcaggtc gcaggggcgg ccgtcgagcc gcgcaacgcg
     2461 cgcagccaga tcgacctcga ggcggccgaa ccggagcacc tcttcggcct gcgcgttcac
     2521 cggtgccgcc cggcgcagca gtgccttgac gcgtgccagc agttcgcgcg gctcgaaagg
     2581 cttgggcagg taatcgtcgg ctccgagttc gagtccgacg atgcggtcca tcggttcccc
     2641 gcgcgcggtc agcatcagca gcggcaggtg gcgggtgcgt gcttcggccc gcagttcgcg
     2701 gcacaggtcg agcccgtcgc catcgggcag catcaggtcg agcaccagcg catcgaagcc
     2761 cggggcgtgc agcggtgcac cgccggcggc agcgagccgg tcacggcctt cggccagggt
     2821 gccggccacc tcgacctcga aaccggccgc acgcaggtag tcgccgacca tggccgacag
     2881 gcgggtgtcg tcgtcgatca tcagcaagcg agctgccatg gatgcttccg ttcgaatgtg
     2941 tcggggccga tccgggtgaa cgcgtggcgg ctgcggtgag ccgccacgcc ggcctgtcaa
     3001 ctcagaagcc gggccggctc cgcgcgctcg ccgtgacgac cacccgatga agctttgtcg
     3061 gcatgcggtc gacccccgtg cgcccgatga tccgccatcc tctgggccag tgtcgcacgc
     3121 tgctccggcg acagcacccg gctcgcgtcg agcatcgcct gcagcgtgcg gcggctcacc
     3181 tgctcgtgct gcgcgagcat ctgctgacgc agctgctcgg ccgcgttggc atcgaccacc
     3241 ggtgcagtga atagctgcac gctgcgctcg tgcaggctgc gtgcggcctc gtgctgcgtc
     3301 ttcagatcgg cggctgcagc cgccgcgatc tgcttgatct gcgtgcgctg cgcatcggtg
     3361 gcatcgagcc cgtcgagcag gcgatcgacg gcacgctcga cgcgttgcgc cggcccggcg
     3421 aacaggcccg gaccgaatcc tccgcgtcca cccatctcgg catggtggcc ccgccggtcg
     3481 ccgtgcgccg gatcatgggc ccaggcggcc acgccgaggg tggccgacaa cgcgatcacc
     3541 gcgccgagtg cccagcgtcc cgccgcaccc tgcaatggcg cgaagtggaa cctgcctgtc
     3601 tttgcttgtg ccatgaatgg ctcctttgaa aacatgatgg gtctgtgcat accggatctc
     3661 gatccggtag ccaccatgct cgcggctcgt cgtgaagctg ccgtggctgc gccgtaaaga
     3721 tgtgtgaatc ccgaggcagg cccggccgac cgcacgcggc cacacgcgcc gcgactactt
     3781 ggtcgaaagc gggaagtccg ggttggcgga aaagaagcct cgcaggtgct tgaggatcag
     3841 cgcgagatcg agcggcttgg tccagtagcc gtccacgcca atctgccggg ccatctcctg
     3901 ctgctgcggc agcgcatcgg cggtgaccgc gatcacctgc atgcgccgcg tggccggttg
     3961 ctggcgaatc tgcttgacga tgtccagacc cgacatgtcg ggcaggttca tgtcggtcag
     4021 cagcagatgg ggctgctcgg ccagcgccag ccgcaggcct tcggccccgg tggccgcctc
     4081 caggagcttc cagttcccca ggcgcccgaa cacctcgcgc atcagcatcg cgttgacggg
     4141 atcgtcctcg atgtaaagag ccgttccgcc tgcggcttcg atccagtcgg tctgcgccgg
     4201 catcgaatcg gtcgaatccc tgccagtttg cgagcccttg gccagtggca gcttgatcgt
     4261 gaagcacgaa ccggtgtgcg gcgtcgaggt gacctcgatc gtgccgccca tgccctgggt
     4321 gagttcgcgg gcgatcacga gtccgaggcc ggtaccctcg atctgggtct tctcggcgcc
     4381 gacccggttg aaaggctgga acagctgatc gacctgtgcc tcgctcatgc cgacgccggt
     4441 gtcctcgatg ctgatctgga ccttcgatgt gcccagcggc tcgacgatga cggtggcctg
     4501 accaccggcg cggttgtact tgatcgcgtt cgacagcaga ttggtgaaca cctggtccat
     4561 cgcacggcgg tcggccttga catgcagctc gtgctcggca ccgatgaact cgacctgcag
     4621 cgcgcgttcg agcgcgagcg gctgcagcaa cgcgatgccg ctgttcacca cggagttgac
     4681 cgacacggat tcgcgctgca gcgagaacat ctgctgctcg atgcgcgaga gttcgagcac
     4741 atcgttgacc agactgagca agcgggtgcc ggacatgcgg atcgtctgga cccgctgctt
     4801 ctggatgctg gtcagcggct cctttcgatc gccttcgagc agctgcgcga agccgaggat
     4861 gccgttgagc ggcgtgcgca gctcgtggct gatgcgcgac agcagctcgc tcttgctgcg
     4921 attggcctca cgggtctcgg ccatctcctg gcgcaggcgc tcgatcgcgt attcctcggt
     4981 gatgtcgcgg cacacgccga tcagcaccgg cgtgcgcgat cgcgtgcggt cgcggctggc
     5041 ctgcgcgcgg gccacgaact cgatgcggcg cggctcgccc gaagtggtga cgacgccgag
     5101 cacctcgcgc tggatcacgc ccgacacggc agccaggctg atgaacgcct gagcgctctc
     5161 gcgcgccgag ggctcgagca tgtccagcca ttgcgccagc acgaatgcgg tgggcgactc
     5221 cgccggcagg tgatgcacct ggcaggcgtt gcggtcgagc gtgatcgttg cggtcgacag
     5281 gttcagctcg aagatgccga tgcccgccgc ctgcgacgcc agctcccact gctgcccgga
     5341 gcgggcgatc tccgcagcag ccttctcgga ctcggtgacg tcggtcgtga atccatgcca
     5401 cagcacgttg ccgtccggca tgcggcgcgg actcgcgaag ccgctcaaca cccgcagccc
     5461 ggcttcgggc agcaccacgc ggaaggtctg cgaccacggc tggccactgt tctcggcaga
     5521 ccggatctgc gcctcgacgc tcttgcggtc ctcggggtgg atgcggtcga acatcggccc
     5581 gctgtccagc gccagcttct ccggcgtgat ctcgaaaagc tgcctgacac tctcgctcgc
     5641 gtaggggcag tagcccgtgc cgtcgggtcg gctcacccgc tggtagagca cgccgggcac
     5701 gtgctgggcc agcaggcgca gcgtgtcgga gacctcgcgt tcctggcgct cggctccgat
     5761 gcggtgcgtg acgtcctgcg ccgatccgcg gtagcccctg aacatcccgt cgctgccgaa
     5821 cttggcgatc gcgctgtacg agatcaggcg gtcccccaag ggggtcggct tcagcacgac
     5881 aacgcgtgcg aaggattgct gccgatcgag gacctcgagg aacccgagca gcggctcgac
     5941 cacccggcca gcaccgtcgc gcatcggcga cgcgggcggg ttctggccga gctgggactg
     6001 caccgtcagt ccggtcaggt cctcgaaccg gtccgacaac caggtgtagt ggtgcgatga
     6061 atcggtttcc cagagccagt cgctggatgc ggaagcaaaa tcgagcagcc tgcgctggct
     6121 gatctgcagc ttgaagatgc gctcgtggct ggcagcggca tcggccgcac cttgcgccag
     6181 gtcctgcaac caccgcatct gctcgggggt cagcgtgcgg ggttgcgccg acatcacgaa
     6241 cagcatgccc agctgctcgc cttcgaactc gatcggatgc ccggcgtagc tgcgcaggcc
     6301 accgtcgcgg ttgaccagcg ggtgctgggc gaaacggaca tcggcagcgg catccaccac
     6361 ctcgaacgtc gtgcctgtct cgatgccatg cgcacagaac gaaagagaca gcggaatgtg
     6421 caccgcctcg agcccgacgc cggccttgaa ccactgccat tcctcgccga ccagactgac
     6481 ggcggcgaca tcgctctgtg tcagctggag cgcgctgcgc acgaacagtt cgaacaccct
     6541 gtcgggctgc gcggcaagca tctgcagctt gcgccggcgg tccgccagca gcacctgtga
     6601 tcgggtggtg cctggctcag tcatgacgcc tgggcgccgc tcaggcccct ctgcgctccc
     6661 gccatcctgc tgcccaccgc aaccatgcct gctcgctccg ccaccgacgt gccccgtcgg
     6721 ccgtgcgcgc agtgtagtgg ctgccagggg cccctccggc gctgcgccac gcggcatcgg
     6781 caaggacgca acaccgcagg ccgaagcggg ccgctcgcag gcagcgaagg ccgggaaact
     6841 gccggccttc ctgcccgggg cgacccggtc ggtcagacct tgtcgatgcg gtccttgagc
     6901 ttctctttcg cgtcaccgaa accggactgg accttgcctg cggtcttgtc gatcctgcct
     6961 tcgatctcga gctccttgtt gccggtgacc ttgccggcga cttccttggc cgcacccttg
     7021 gcctgctcga cgcgaccctt gacctgatcc ttgttcatgc acgactcctg ttcattcaaa
     7081 gtggatggaa tcggtcggcg ctctgtccag agcaggcccc gtccgacgaa acaactttgc
     7141 cgacgcgcgt gcgccaggtc tgtcgggatg tcgcgcaggg atctgtagga cgatgggccg
     7201 gcacatcggc cgccgcatcg gcgggcagct gatgatcgac cggttcagcc tgcgacttgc
     7261 tgcagccggg ctgccagcgc gcggacatcg gcatccggcg gctcgccacc gaggtgggtc
     7321 tggccgtcga gtgccgcctg aacgagacgc gccagcgtgg acgcgtgggg agtgatggtg
     7381 ccgaagaaaa gcacttcgcc gccacgggcc atcagcagcg tcgggaaatt gtcgacatcg
     7441 acggcgccga ccagatcgga ctcgtcctcg acatcgatcc acaacccccg aacgcccgcg
     7501 tcgcgccggc cctgcaccgc ctgctcgaag acagtccggt attcgcggca ggtgccgcac
     7561 cactcggcac acaggcaggc cacggtgatg gaggcagggg gcaggtggtc gacaggctcg
     7621 ctcatcgttt cagggttgca ggatcagttt gcacgccagt gtgcccgttg cgcgccatcg
     7681 tcagccccgc cgactcagcc gccggcacgc ttgaccttca tgcccgccgc ctccaaccgc
     7741 tcgacgatcc ggtcgcggtg gtcgccctgg atctcgatga ccccgtcctt gacggtgccg
     7801 cccgagccgc aggcagcctt caggtcgcga ccgagctgcg ccagtgctgc ggcatcgagt
     7861 accacaccgc ggatcaccgt cacacccttg cccttgcggc cctgcgtctc gcgagaaacg
     7921 cggacgatgc cgtcggtggc gaccggcgcc gtcgcctgac cgcagcgaca ctgcgcaacc
     7981 ggctgccggc atgccgggca catgcggccg ctgtcggtcg aatagaccag gcctgaagtg
     8041 ctgcttcgat tcttcatggg ggacgcgctc ggactgattc tcgcgcaccg cagtgtgcac
     8101 ggcgccgcgc agcgcccatt gaaaacccgg cccggagcac ccagcttcga gcggtctcac
     8161 acccgcgttt cgctgatccc ttggcattgc tgatcctctc caccgccacg ctgctgctgt
     8221 tgacgtggct gctgcgcgag ccgttgttca ccgaatggcg ccggcgcaag gtgcgccggc
     8281 agccctttcc ggccgaatgg cggcaggttc ttcgccatcg tgtgccctac ttccgctcga
     8341 tgcctgccga tctgcagttg cagctgaaga agcacatcca ggtgtttctt tccgagaagc
     8401 ccttcatcgg ctgccgcggc ctggtcgtca ccgacgagat gcgtgtgacg atcgcggcgc
     8461 aggcctgcct gctgatcctc aatcgacgct gcgactactt ctcacagctc cggcagatcc
     8521 tgctgtatcc gggagcattc gtcgtcgacc gcgtgcatgc agacggcagc ggggtggtgc
     8581 aggaccgtcg gctggcgctg tccggcgagt cgtggtcgct gggtcaggtg atcctgtcct
     8641 gggaagacgc agtcgagggc gccgaggtgg ccgacgacgg ccgcaacgtc gtgatccacg
     8701 aattcgcgca tcagctcgac caggagagcg gcgcggccaa cggatcgccc cgcatgcccg
     8761 ggcgtgccag gcgcgagcgc tgggcgcgcg tgctgagcga agagttcgcc gtgctgcaac
     8821 gacgcgcgag cgagcgacgc ccgagcctgc tcagcgacta cggcgccacc gacccagcgg
     8881 aattctttgc ggtggcgtcc gaggtgttct tcgagcagcc gcaacgcctc gctgccgagc
     8941 acgcagcgct gtaccgcgaa ctcagccact actaccgggt gaatccgctg agctggtagc
     9001 tgcgattcgc gacaaccgtg catcgtggca agaaaatcga ggcactgctg agtgcacgtc
     9061 ccggcgaaat cacgcaccta gaatcgccga atgcatctcc gtcagcgcag cacgcagctc
     9121 attctgtgga tcgcatgcct ggcactcgcg gtcggggcgt tgctcccgtc ggtaacccat
     9181 tcagtcagac aagcggcgcc gaacggttgg ttcgaggtct gtaccagctt cggcgccaag
     9241 tgggtccgcg cgagcgaagc tcgcactgac tcggaaggac ccgaggcgcc acctcggggc
     9301 atccacgagc actgcgcgta ctgcgtcatg catgccggag atctggcagt gccgccagcg
     9361 cgtggcaccg ggctggctcc cagcccgctc cagttcgctt tgccagcacg gttcctcgcc
     9421 gcacctcgaa cgctgttcgc gtggcgttct gcccaggcgc gtgcgcctcc ccgggtcgcc
     9481 tgacctgccg agatcgtcgc cgcggactcc gagtctgctg cggttgcggc cggcttgacg
     9541 tcgaagtatc tcggaccgtc gacaggcaat tgaacgcccg gcgcccttga ctggcgcggg
     9601 ccctgtcttt gtcgggcgtc actgcgacga cacgcagccg aaatcaccgt ccaaccatcg
     9661 cgcgaggtgc ggccgccgca cggccacacc tcgttcgccg aagtgaactg actcctcatc
     9721 cccatcatga aaaaatcccg caacagcctt tcagccgccc gacggcgccg catcacttca
     9781 ccgacctcgt tgctggcggc tgccgcggcg ctgagcggga tcatcccgct tgacgtggcc
     9841 tcagccgagc ccgcaactcg cgattcgaca ctcacgctcg gcagcgtgac cgtcacagga
     9901 cgctccagcg gcccgctgcc cgtgcgcagc gtgctgagtt cggtggacat cctgggcgag
     9961 gacctgctgc gcaaccagca ggtcaacaac agctgggaac tcttcggccg cgcgccaggc
    10021 gtgcttctga ctccattcaa gcagggcaac gtctccggca agttctcgtt tcgggcgttc
    10081 aacggagaag gcgaactgaa tgccgtcaag ctgctgatcg atggcatccc gagcaacgcc
    10141 aacgacggca gcatgcagtt cctcgacatg atctcgccgc ttgagatcca gagactggaa
    10201 atcgtgcgcg gcacgaacga cgcacgctac ggtcttcaca acatcgccgg caacgccaat
    10261 gtggtgacac gccagggcgg caacgagacg ctggccagcg taggcatcgg gagcttcaac
    10321 acccgcgagg ggcagctcgc ccatggcatc gagggcgaca actggtcgca gaactatttc
    10381 atcggctacc gaaaaagcgg caactaccgc gaccacgccg attccgacaa gctcacgctg
    10441 agcggcaagt ggttctacac aaccgatgac agccggttca gggctggtct gatcgcacgg
    10501 cactatcaca acaacgcgga cgagcccggc tacctgaccc gagccgatgc ccgcgatcac
    10561 ccgcgacagt cgtatgcgct gtcgtcgacc gacggtggca agagggacat ggatgcgctg
    10621 agcgcccacc tcgacgtccg actgaccgac gaggtctcgt ggtcggcaaa ggcctacggg
    10681 aacgagttga aggacaagcg atacatacgc ttctcggcct ccgcttcgca gcaggagcgc
    10741 gtcaccgacg agacccagta cggggcactg acctcgttgt cgtggcgccc gaaggtgacg
    10801 tgggcgcgcg agttttcgct cgaaggtggc gccgacatca accgcgaaca caacaacagc
    10861 ctgcgttacc tgaccgcaga acgcgcaagg cagagtcaga ctcgtgacca gtcattcagc
    10921 ttcgacaccc agggagccta tgtccaggct gtgatcgcac ccatcgaatc cctgaaggtc
    10981 atcccggcat accgcatcga cgacgtggaa ggcagcttca cggatcgact gactcggcga
    11041 acttctccga tcaacgactt cggactgatc aagcagccca aggtcagcgt ggtttacgcc
    11101 ccgtggcaac agggcagcgt ctacggcaac tggggtcgca ccttccaggt cggcgccgga
    11161 tcggcggcct acaagattcc accccgcacc gcggacctga agccctccat caacgaaggc
    11221 tgggaggccg gcgtcaagct gcaaccgact gactggatcg atggtcgaat cgctgtctgg
    11281 gagcagaagg cgtcggacga ggtccgccgc aagctcaacg atccggcagg cgactccgag
    11341 aacgtgggca agacgcgacg ccggggcctg gacctgcagg tcaacctgcg gccggagaag
    11401 cggatcaatg cctggttcgc ctactcggca cagcgctcca ggatcatcga acccgatccc
    11461 gccgcaccga ccactcgagg caagcagatc gatcatgtgc cgcgccatct gctgtccggc
    11521 ggcgtggatg tccaggcctt gcccgagctg cgcctgtggg catccgcgaa cggacagagc
    11581 agctacttcc tcgagcgcac gaacagcacc gggaaattcg gcagctacct gctgctgaac
    11641 ctgggcgtct cgtatcagct cacgcgcagc atcagcgtcg acgcgcagct gaagaatgtt
    11701 gcggacaggt acaccgaata cgcttggtgg gacggcgcgc agtcgctgca ttcacctggc
    11761 gacggacgag cgttctatct caccctgaac tggaaactcg accgatgagc cagcgctcca
    11821 ccgggccggt gaacgacagc ctctatcgcg cggtgtggcg atggcacttc tatgccgggc
    11881 tcctggtctt gcccttcctg gtgctgct
//
LOCUS       DAOORV010000109        11653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6876_length_11653_cov_7.383256, whole genome shotgun sequence.
ACCESSION   DAOORV010000109 DAOORV010000000
VERSION     DAOORV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_11653_cov_7.383256"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="lpxK"
                     /locus_tag="PKV56_13335"
     CDS             <1..883
                     /gene="lpxK"
                     /locus_tag="PKV56_13335"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOX68841.1"
                     /translation="GAPVIVVGNLIAGGAGKTPTVIAVVRCLQAAGRSPGVISRGYGR
                     QADAPPVLEVQPDSPAARVGDEPLLLRLRLKVPVFVGADRVAVASALLRSHPGVDVLV
                     SDDGLQHLRLPRAAQVVVFDERGAGNGWMLPAGPLREPLANQVPPQTVILYNAPRPTT
                     PWPGHLATRRVSGLIGLADWWAGRPATMAALTALIAEGKPVIAAAGISQPERFFGMLR
                     AAGLDITPLPLPDHHAYATLPWPSDAPHVVVTEKDAVKLRPEQFAAGPASTRVWVAPL
                     DFCGDASFDTALMALLH"
     gene            908..1147
                     /locus_tag="PKV56_13340"
     CDS             908..1147
                     /locus_tag="PKV56_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOX68842.1"
                     /translation="METRLLDLLVCPICKGPLEHWRPPHQERQELVCHADKLAYPVRD
                     GIPVMLESEARTIGNEPPPAPIFPMPPATGSASPQ"
     gene            1144..1896
                     /gene="kdsB"
                     /locus_tag="PKV56_13345"
     CDS             1144..1896
                     /gene="kdsB"
                     /locus_tag="PKV56_13345"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOX68843.1"
                     /translation="MSFSVLIPARLASTRLPRKPLADIAGLPMVVRVARQAALSSAAR
                     VVVAADDAEIVAACEAHGVTAILTRSDHVNGSDRLAEACILLGLDGEAVVVNVQGDEP
                     LIDPALIESCAAALTAAPDCVMSTAAHALTQSGELANPNIVKVVCDRAGRALYFSRAP
                     IPWWRDGSTAGAPAIGPLAPLRHIGIYAYRAAFLRRFPSLPVSPLETVESLEQLRVLW
                     HGERIAVHVSEQPAGIGVDTPEDLQRVRELMA"
     gene            2000..2653
                     /gene="adk"
                     /locus_tag="PKV56_13350"
     CDS             2000..2653
                     /gene="adk"
                     /locus_tag="PKV56_13350"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOX68844.1"
                     /translation="MRLILLGAPGAGKGTQATFICKKFGIPQISTGDMLRAAVKAGTP
                     LGIAAKKVMDSGGLVGDDIIIGLVKERLAQPDCANGFLFDGFPRTIVQADAMKAAGVN
                     LDIVLEIDVPDEAIVERMSGRRAHLASGRTYHVKFNPPKVEGIDDVTGEPLIQRDDDK
                     EETVKKRLDVYQKQTRPLVSYYSDWSASGDPVAPKYRKISGTGSVDEIMARALQALS"
     gene            complement(2729..3157)
                     /locus_tag="PKV56_13355"
     CDS             complement(2729..3157)
                     /locus_tag="PKV56_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX68845.1"
                     /translation="MAMNIGSGRSDEPEVMMDINTTPLIDVMLVLIIMLIITIPVQLH
                     SVNLNMPHGNPPPPVRPPVVVTIDIDVGGLVLWNGEPLADRQALEARLKLAAAQPDQP
                     EVHLRPNKAVDYGVVAMVLASAQRLGVTKLGMVGNEQFAP"
     gene            complement(3180..3605)
                     /locus_tag="PKV56_13360"
     CDS             complement(3180..3605)
                     /locus_tag="PKV56_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX68846.1"
                     /translation="MSFHLRPASDDDEVVASINTTPLVDVMLVLLIIFLITIPVVTQT
                     VPMSLPKEVNRARQTKPENIEISVDRAGAVYWNAQPVADHAALVARLKDIGVKNPQPE
                     VHIRGDEKSRYAAVGRVLYACQRAGILKISFVTEPPPKG"
     gene            complement(3626..4489)
                     /locus_tag="PKV56_13365"
     CDS             complement(3626..4489)
                     /locus_tag="PKV56_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX68847.1"
                     /translation="MTMFNTLVRRLERASGSRCLTWLFACVLLAAPVCLWQPAWAQDV
                     AASAAAAQAAAPGLASAVVEQQVVDNPYGLGALWAQGDFVSKGTLVILVLMSMASWYI
                     LVTKLYASFQIAADARLARNEFFKASSLQSGAQLLKDGSPFRYIAQNGIDADERHEGS
                     LTANIDRNTWVTMSVQRSIDEVQSRLQDGLAVLATVGSTAPFIGLFGTVWGIYHALTA
                     IGIAGQASIDKVAGPVGEALIMTAIGLAVAVPAVLGYNWLVRRNKITMDAVRGFGADL
                     HGILMGAKGVR"
     gene            complement(4550..5239)
                     /locus_tag="PKV56_13370"
     CDS             complement(4550..5239)
                     /locus_tag="PKV56_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOX68848.1"
                     /translation="MDFSKRQDDPRRHLLGLSVVVALHVLIVYALVNGLARKVVDVVR
                     APIETRLIEEIIKPPPPPPEPPPPPPPERVVPPPPKVAPPPPPFVPPPEVTVTAPPPV
                     APTITAVTPTPPPAPVEITPTPPVVEPEPAPPAKPAQPVAAGIVCTRMPAPVAPSVAY
                     EVKGSLFVIGTLRNGRVVQVDVERNTLKGVNDRRTQRAFVSAVETAMKDGYVCAGDGV
                     QIRQEFFFDIR"
     gene            complement(5246..8029)
                     /locus_tag="PKV56_13375"
     CDS             complement(5246..8029)
                     /locus_tag="PKV56_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX68849.1"
                     /translation="MRTDLQTRRRRAWPATLALSCLCVLEPVMGQQALDRVEITGSSI
                     RRIDAESALPVQVIKRETIERSGYTSTVDLFRNLPAVMGSTVESATVGGETYGFAGVS
                     IHNLGENRTLVLLNGRRLASFGGQTLTGASNAIDLNSIPISAIERIEVLADGASALYG
                     SDAIAGVVNFITRRGGTDGVGTAGVSYPRGGARETNISVSKGFGDLETDGYNLMFSAS
                     SDRRTQLAASERKFARTGEIFFRQGGQKYRFQASSILGIPANVFDYDFNAYSPYLQAN
                     GKCPARHFVSGSACAYDFSSDLEIYPDRERQSLVASFDRALAGDHRLFGDVVFARSRS
                     RGTVSYNPSLLTISPDSPYFDQAVAAGGYNNTDPGQGDLGVLAGYRFTDLGRRVTDDR
                     SEAFHAVVGVAGTLGTWDYNVAYTHSQSTVRGSLDDGYGYNSRVLQALNTVVNPFALP
                     GQQSAEARQAIADAKYTGYWNGGRSTLDGLEARGSTELLALPAGAMQLGAGVSAFHER
                     ISTRPSALAQAEGGEVRFGDDAAEVPSRGSRNVLGAFAELVAPVARDVELTASVRHDH
                     YSDFGNTTNAKLSGRWQPLPGLLIRGSVGMGYKAPSILQVKASRQKYGLSGGGLDCGL
                     PDARGVTLNDIAASLGSDVSCVDGVQADVIAQGNHDLKPERSRQASLGFRIEPVSTLS
                     FGADFWTVGIKDTIGQLSEGAIFSDYSLYRDSFTSSVSAATGRKQLALFAPNLNQGKS
                     YTSGIDFDLVGRMKLPAGVGLTSQLTATYMLRDQYQSLEGGDYASSLGRLGENGAVTF
                     RWQARWVNSFVVGPATHTLALNYKSGYRDQDFAATGQVDVNVVNADGSIGERVDSLKR
                     RVSDHLTVDWQTHWRMSNLMTLNFGVINVFDKDPPLSMTVGGFNQGFQIGYDDRYYDP
                     RGRTLYANLSYRF"
     gene            complement(8118..9998)
                     /locus_tag="PKV56_13380"
     CDS             complement(8118..9998)
                     /locus_tag="PKV56_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68850.1"
                     /translation="MLNITDLQVAIDVDAGMIRAIDGLSLCIERGETFALVGESGCGK
                     SVTALALMRLLPDNARVTEGRVEIDGQEALSLPESAMRRLRGGKVGMIFQEPATSLNP
                     VMRIGDQIVEAIETHTALRGAVARAKAIEWLGRVGIPEPERRIDDYPFRMSGGQKQRV
                     MIAIALAAEPSFLVADEPTTALDVAIQAQILALLKQLRREQGMGMLLITHDLAVVSGM
                     AQRVALMYAGQIVEVAAAAEFFGDPKHPYARLLLQSLPGAHKRGEVLAAITGTVPALS
                     QPFTGCRFAPRCDRVFDRCRAEVPVLLRPAGALLSLAPPAALPPREVRCWLHVVPGSA
                     EAAEDDGPTSRSAAVATPVVAASAPGWLLDVQDLSVRFPVRTGILQRVSSHAVAVSNV
                     SLQIRHGETFALVGESGCGKTTLGKAIVQLLRGQAVIEGRALLDGQDLFGLHGDALRQ
                     ARRSVQMIFQDPYASLNPRMRVLDVLEEGLLSLRPELDASARRQLLETLVDRVGLRRE
                     ALQRYPHEFSGGQRQRIAIARALAVEPRLIVCDEPTSALDVSVQAQILNLLLELQREL
                     GLSYLFITHHLGVVEYIADHVAVMYRGRIEESGPAASLLAAPREEYTRRLLAAVPRLV
                     RA"
     gene            complement(10005..11456)
                     /locus_tag="PKV56_13385"
     CDS             complement(10005..11456)
                     /locus_tag="PKV56_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX68851.1"
                     /translation="MPKFVLLWTDAVMWALVVALLVYARVVWRSPNLRASWTLVFRDA
                     AAFASAIVLGLCLVLTLLDSIHFRRALPAASTGSPTAAKAYDTRTQSALDSLLSRQIE
                     SREATYSRPLAYQSFTLESLTVDGEVRRLPPRLLHGGAHLRDPQRQWAGDIGARIAAG
                     LAIGAGAALLLGAWIVLGVARRNGWPWGEALGRVLRDETDIRWRAALMMLTVLCLLVG
                     PAALLMNDYHLLGTDRTGNDVLYQALKSIRTAFVIGTLATVATLPLAVGLGLMAGYFK
                     GWVDEAIQYLYTVLSSVPNVLLIAACVLMVQVFLDQHPEMFETGAERADLKLFLLCAI
                     LGVTGWAGLCRLLRAEALKLRELEYVQAATAFGVGPWRIMARHLLPNVMHLVLITTVL
                     DFSGLILYEAVLSYVGVGVDPSMNSFGGMINLARSEMSRDPLVWWSFAAAFGFMVTVV
                     LAANFFADGVRDAFDPRARVYRARRAPATGKAG"
BASE COUNT         1928 a         4000 c         3918 g         1807 t
ORIGIN      
        1 cggcgcgccg gtgatcgttg tcggcaacct gatcgccggc ggcgccggca agacacccac
       61 cgtgatcgcg gtcgtgcgtt gcctgcaggc ggcgggccgc tccccgggag tcatctcgcg
      121 tggctacggc cggcaggctg atgcaccgcc ggtgctggaa gtgcagcccg actcgccggc
      181 ggcccgggtc ggggacgagc cgctgctgct gcgacttcga ctgaaagtac cggtgttcgt
      241 cggcgccgat cgcgtggcgg tggcctcggc cctgctgcgc tcgcatcctg gcgtcgatgt
      301 gctggtcagc gacgacgggc tgcagcacct gcggctgccg cgtgcggcac aggtcgtcgt
      361 gttcgacgaa cgcggtgctg gcaacggctg gatgctcccg gccgggccac tgcgcgagcc
      421 gctggcgaat caggtacctc cgcagaccgt gatcctctac aacgcgccgc ggccgacgac
      481 gccctggccg ggacatctgg cgacgcggcg ggtctcggga ctgatcggtc tggccgactg
      541 gtgggccggc cggccggcaa cgatggcggc cctgacggcc ctcatcgccg aaggcaagcc
      601 ggtgatcgcg gctgcgggca tttctcaacc tgagcgcttc ttcggcatgc tgcgagcagc
      661 gggattggac atcacgccgc tgccgctgcc cgatcaccat gcctacgcca ccctgccctg
      721 gccctcggat gcgccgcatg tcgtcgtcac cgagaaggac gccgtcaagt tgcggcccga
      781 gcagttcgcc gccgggcccg cctccacacg cgtctgggtc gcgccgctag acttctgcgg
      841 cgatgcgtcc ttcgacacgg cactgatggc gctgctgcac tgacctcaaa cgacccaagg
      901 acacccgatg gagacccgac tgctcgacct gctggtgtgc ccgatctgca agggcccgct
      961 ggagcactgg cgcccgccgc atcaggagcg ccaggaactg gtttgccatg ccgacaagct
     1021 ggcctacccg gtgcgcgacg gcatcccggt gatgctggaa agcgaagccc gcaccatcgg
     1081 caacgaaccg ccgcctgcac cgatcttccc gatgccaccc gccaccggat ccgcctcccc
     1141 gcaatgagct tttcggtgct gatcccggcg cgactggcct cgacgcgatt gccgcgcaag
     1201 ccgctcgccg acatagccgg cttgccgatg gtggtgcgcg tcgcacggca ggccgcgctg
     1261 tcgtcagcgg cacgggtcgt cgtggcagcc gacgacgccg agatcgtcgc cgcctgcgag
     1321 gcccacggcg tcaccgcgat cctgacgcgc agcgaccatg tcaacggcag cgaccggctg
     1381 gccgaggcct gcatcctgct ggggctcgat ggcgaagcgg tggtggtgaa cgtgcagggc
     1441 gacgaacccc tgatcgatcc ggccctgatc gagtcctgtg ccgccgcgct gacggcagcg
     1501 ccggactgcg tcatgagcac ggccgcacac gcgctgacgc aatccggcga gctggcgaac
     1561 ccgaacatcg tcaaggtggt ctgcgaccgg gccggccgcg cgctctattt ctcgcgcgcg
     1621 ccgattccgt ggtggcgcga cggcagcacc gcgggcgcac ccgccatcgg cccgctcgcg
     1681 ccgctgcggc acatcggcat ctatgcctac agggcggcat tcctgaggcg tttcccgagc
     1741 ctgcccgtga gcccgctgga gacggtcgag tcgctcgagc agttgcgcgt tctgtggcat
     1801 ggcgagcgca tcgcggtgca cgtgagcgag cagcccgccg gaatcggagt cgacacaccc
     1861 gaagacctgc aacgggtgcg cgaactgatg gcgtgaaacc cgcaggggcg aaacgtccgg
     1921 ttcggtttta gactacccgg tccggcccga atgcatgtcg gagcatcgcg attcaatcga
     1981 ccagataaca aggacaccca tgagactgat tttgttgggc gcgcccggtg cgggcaaggg
     2041 aacgcaggcc accttcatct gcaagaagtt cggcatcccc cagatctcga ccggcgacat
     2101 gctgcgcgct gccgtcaagg cgggcacccc cctgggcatt gccgccaaga aggtcatgga
     2161 ttcgggcggc ctggtcggtg acgacatcat catcggcctc gtcaaggagc gcctggccca
     2221 gcccgattgc gccaacggat tcctgttcga cggcttcccg cgcaccatcg tgcaggccga
     2281 tgcgatgaag gcggcaggcg tcaacctcga catcgtgctc gagatcgatg tgcccgacga
     2341 ggccatcgtc gaacgcatga gcggccgccg ggcccatctg gcctcgggcc gcacgtacca
     2401 cgtcaagttc aacccgccca aggtcgaggg catcgacgac gtcaccggcg agccgctgat
     2461 ccagcgcgat gacgacaagg aagaaacggt caagaagcgt ctcgacgtct accagaagca
     2521 gactcgcccg ctggtgtcct actactccga ctggtcggcc tcgggcgatc cggtggcacc
     2581 gaagtaccgc aagatcagcg gcaccggcag cgtcgacgag atcatggcgc gcgccctgca
     2641 ggcgctgtcc tgacccaggc ctgaccactc cctgatccac caccgatgcc cgccggtcgc
     2701 ctcgtgcgat cggcgggcat cggctttttc agggcgcgaa ctgctcgttg ccgaccatgc
     2761 ccagcttggt cacgcccagg cgctgtgcgc tggccagcac catcgccacc acgccgtagt
     2821 cgaccgcctt gttggggcgc aggtggactt cgggctgatc gggctgggcg gcggcgagct
     2881 tcagccgcgc ctcgagcgcc tggcggtcgg ccagtggctc gccgttccac agcaccaatc
     2941 cgccgacatc gatgtcgatg gtgacgacca caggcggccg gacgggtggc ggcggattgc
     3001 catgcggcat gttcaggttg accgagtgca gctgcaccgg gatcgtgatg atcagcatga
     3061 tgatcagcac cagcatcacg tcgatcagcg gcgtggtgtt gatgtccatc atcacttcgg
     3121 gttcgtcgct gcggccggag ccgatgttca tcgccatgaa aggttccttc tgcgcgggct
     3181 cagcccttgg gcggcggctc ggtgacgaag ctgatcttca ggatgccggc gcgctggcag
     3241 gcatacagca cgcgacccac ggcggcgtag cgtgatttct cgtcgccgcg gatgtggact
     3301 tcgggctgcg ggttcttcac gccgatgtcc ttgagccgcg cgaccagcgc ggcgtgatcg
     3361 gccaccggct gggcgttcca gtagacggcg ccggcacgat cgaccgagat ctcgatgttc
     3421 tccggcttgg tctgccgcgc ccggttgacc tccttgggca acgacatcgg caccgtctgc
     3481 gtgacgacgg ggatggtgat caggaagatg atcagcagca ccagcatcac gtccaccagt
     3541 ggcgtggtgt tgatgctggc gacgacctcg tcgtcgtcgc tggccgggcg gagatggaag
     3601 gacatggcgc gtggcccgga gcgtgtcagc ggacgccctt ggcgcccatc aggatgccat
     3661 gcaggtcggc cccgaagccg cgcaccgcat ccatcgtgat cttgttgcgc cgcaccagcc
     3721 agttgtagcc gagcaccgcc ggcaccgcga ccgccaggcc gatcgccgtc atgatcagcg
     3781 cctcgccgac cgggccagcc accttgtcga tgctggcctg cccagcgatc ccgatggcgg
     3841 tgagcgcgtg atagatgccc cagacggtgc cgaacaggcc gatgaacggc gcggtcgaac
     3901 ccacggtggc cagcaccgcc agaccgtcct gcagccgcga ctgcacctcg tcgatgctgc
     3961 gctgcacgct catcgtgacc caggtgttgc ggtcgatgtt cgcggtcagc gagccttcgt
     4021 ggcgctcatc cgcgtcgatg ccgttctggg cgatgtagcg gaacgggctg ccgtccttca
     4081 gcagctgcgc gccggactgc aggctggacg ccttgaagaa ttcgttgcgc gccagacggg
     4141 catcggccgc gatctggaag ctcgcataca gcttggtcac caggatgtac cagctggcca
     4201 tgctcatcag caccaggatg acgagcgtgc ccttcgagac gaagtcgccc tgggcccaca
     4261 gcgcacccaa tccgtagggg ttgtcgacca cctgctgctc gaccaccgcc gacgccagac
     4321 cgggcgcagc agcctgcgcc gccgcggcag aagctgccac gtcctgcgcc catgccggct
     4381 gccacaggca gaccggcgca gccagcagca cgcatgcgaa caaccacgtc aggcaccgcg
     4441 accctgacgc tcgttcgagc cggcgcacaa gagtgttgaa catcgtcatg gggatctccg
     4501 gaaaatcagt gctggaagcg gttcaagaat gaggcgacgg cggcggccgt caacggatgt
     4561 cgaagaagaa ttcctggcgg atctgcacgc cgtcgccagc gcacacgtag ccgtccttca
     4621 tggccgtctc gacggcgctg acgaacgccc gctgcgtgcg tcggtcgttc acgcccttga
     4681 gcgtgttgcg ctcgacgtcc acctgaacga cgcgcccgtt cctgagcgtg ccgatcacga
     4741 acaggctgcc cttgacctcg taggcgacgc tgggtgcaac tggcgcaggc atgcgggtgc
     4801 acacgatgcc ggcggcgaca ggctgcgctg gcttggcagg tggcgcaggt tccggctcga
     4861 ccaccggcgg cgtcggcgtg atctcgaccg gcgccggggg tggagtcggc gtgaccgcgg
     4921 tgatcgtcgg cgccacgggc ggcggtgcgg tcacggtgac ctcgggcggc ggaacgaacg
     4981 gcggcggcgg cggggccact ttcggcggag gtggcacgac gcgctcgggt ggcggcggtg
     5041 gcggtggctc gggcggagga ggcggcggct tgatgatctc ctcgatcagc cgggtctcga
     5101 tcggcgcgcg caccacatcg accaccttcc tcgccaggcc attcaccagc gcgtagacga
     5161 tcagcacgtg cagcgccacg acgacggaca gacccagcag atggcggcga ggatcgtctt
     5221 gccgcttcga gaaatccatg aaagatcaga accgatagga gaggttggcg tagagcgtgc
     5281 gcccgcgggg atcgtaatag cgatcgtcgt agccgatctg gaatccctgg ttgaagccgc
     5341 cgacggtcat cgaaagcggc ggatccttgt cgaacacgtt gatgacgccg aagttcaggg
     5401 tcatcagatt cgacatccgc cagtgtgtct gccagtcgac cgtcaggtga tcggacaccc
     5461 ggcgcttgag cgaatcgaca cgctcgccga tcgagccatc ggcgttgacc acgttgacat
     5521 cgacctgccc ggtggccgcg aagtcctggt cgcggtagcc cgacttgtag ttgagcgcca
     5581 gcgtgtgcgt ggccggaccg acgacgaacg agttgaccca gcgtgcctgc cagcggaagg
     5641 tgaccgcgcc gttctcgccc agtcgcccga ggctgctggc gtagtcgccc ccttcgagcg
     5701 actggtactg gtcgcgcagc atgtaggtgg cggtgagctg cgaggtcagc ccgacgccgg
     5761 caggaagctt catgcggccg accaggtcga aatcgatgcc cgaggtgtag ctcttgccct
     5821 ggttcaggtt gggcgcgaac agcgccagct gcttgcgccc ggtggccgca ctgaccgagc
     5881 tggtgaagct gtcgcggtac agcgagtagt cgctgaagat cgctccctcg ctgagctggc
     5941 cgatcgtgtc cttgatgccc accgtccaga agtcggcacc gaaggacagg gtcgacaccg
     6001 gctcgatgcg gaagcccagc gaagcctgcc tcgaccgctc gggcttcagg tcgtgattgc
     6061 cctgcgcgat cacgtcggcc tgcaccccat cgacgcagct cacgtccgac cccagcgacg
     6121 ctgcgatgtc gttgagcgtc acgccacggg cgtcgggcag accgcagtcc aggccgccgc
     6181 cgctgagtcc gtacttctgg cgcgatgcct tcacctgcag gatcgacggg gccttgtagc
     6241 ccatgccgac cgagccgcgg atcagcaggc cgggcagcgg ctgccagcga ccggacagct
     6301 tggcattggt ggtgttgccg aagtccgagt aatggtcgtg ccgcaccgat gcggtcagtt
     6361 ccacgtccct ggccaccggc gccaccagtt cggcgaacgc gcccagcacg ttgcgcgagc
     6421 cacgcgaggg cacctcggcc gcatcgtcac cgaagcggac ttcgccgccc tcggcctgtg
     6481 ccagcgcgct gggccgcgtg ctgatgcgct cgtggaacgc gctgacgccg gcacccagct
     6541 gcatcgcacc cgcgggcagc gcaaggagtt cggtgctgcc ccgggcttcc agtccgtcga
     6601 gcgtcgagcg gccgccgttc cagtagccgg tgtacttcgc gtcggcgatc gcctggcgcg
     6661 cctcggcgct ctgctgcccc ggcaacgcga acggattgac caccgtgttc aacgcctgca
     6721 acacccggct gttgtagcca tagccatcgt ccaggctgcc ccgcaccgtg ctctgcgaat
     6781 gcgtgtaggc gacgttgtag tcccaggtgc ccagcgtccc cgcgacaccc accaccgcgt
     6841 ggaaggcctc ggagcggtcg tcggtgaccc ggcgccccag gtcggtgaac cggtagccgg
     6901 ccagcacgcc gagatcgccc tggccgggat cggtgttgtt gtagccgcca gccgccacgg
     6961 cctgatcgaa gtaaggcgag tccggcgaga tggtcagcag gctcgggttg tacgaaacgg
     7021 tgccccggga gcggctgcgc gcgaacacga cgtcgccgaa caggcggtgg tcgccggcca
     7081 gcgcgcggtc gaaggaagcg accaggctct gccgttcgcg gtcggggtag atctcgaggt
     7141 cgctgctgaa gtcgtaggca caggcgctac ccgagacgaa gtgccgggcc gggcacttgc
     7201 cgttcgcctg caggtagggg ctgtaggcat tgaagtcgta atcgaagaca ttcgccggga
     7261 tgccgaggat cgacgatgcc tggaagcggt acttctgacc gccctgcctg aagaagatct
     7321 cgccggtgcg tgcgaacttg cgctcggacg cggccagttg cgtgcgccgg tcggaactgg
     7381 ccgagaacat caggttgtag ccatcggtct cgaggtctcc gaagcccttc gacacgctga
     7441 tgttggtctc gcgtgccccg ccgcgcggat acgagacgcc cgccgtgccg acgccgtcgg
     7501 tgccgccgcg ccgggtgatg aagttgacga ccccggcaat ggcatccgag ccatacagtg
     7561 ccgaggcacc gtccgccagc acctcgatgc gctcgatcgc cgagatcggg atgctgttca
     7621 ggtcgatggc gttcgacgcg cccgtcagcg tctggccacc gaaggacgcg agcctcctcc
     7681 cgttcagcag caccagcgtg cggttctcgc ccaggttgtg gatcgacacc ccggcgaacc
     7741 cgtaggtttc accgcccacg gtggcggatt cgaccgtcga gcccatgacc gccggcaggt
     7801 tcctgaagag atcgacggtc gacgtgtagc cgctgcgttc gatcgtctcg cgcttgatca
     7861 cctggacagg cagcgccgac tcggcatcga tgcggcgtat cgacgatccc gtgatctcga
     7921 cacgatcgag cgcctgctgt cccatcaccg gttcaagcac acacaagcat gacaacgcca
     7981 gtgtggctgg ccaggctctg cggcgcctgg tctggaggtc ggtcctcatc ttgtctcctg
     8041 ttcgcggcat cgagtttcgc cgattccgcg accgtgccga acggaggtct gccacgcgcg
     8101 actcaggcac caaggcgtca tgccctgacc aggcgcggca ccgcggccag caggcggcgg
     8161 gtgtactcct cgcgcggagc ggccagcaac gaggcggcag gccccgactc ctcgatgcgc
     8221 cctcggtaca tcaccgccac gtggtcggcg atgtactcga ccacgcccag atggtgcgtg
     8281 atgaagagat aggacagccc gagttcgcgc tgcaattcga gcagcaggtt gaggatctgc
     8341 gcctgcaccg acacgtcgag cgccgaggtc ggctcgtcgc agacgatcag tcggggttcg
     8401 accgccagcg cgcgcgcgat cgcgatgcgc tggcgctggc cgccggagaa ctcgtgcgga
     8461 tagcgctgca aggcctcgcg ccgcagaccg acccgatcga ccagggtctc gagcagttgc
     8521 cgacgcgccg acgcgtcgag ttccggacgc aggctgagca agccctcctc cagcacgtcg
     8581 agcacgcgca tccggggatt cagggaggca taggggtcct ggaagatcat ttgcaccgaa
     8641 cggcgggcct gtcgcaacgc atcgccgtgc agcccgaaca ggtcctgccc gtcgagcagc
     8701 gccctgccct cgatcaccgc ctgaccgcga agcagttgca cgatcgcctt gcccagcgtg
     8761 gtcttgccgc agcccgactc gccgaccagc gcgaaggtct ctccatgtcg aatctgcagc
     8821 gacacgttcg acaccgccac cgcatgcgag ctgacacgct gcaagatacc ggtgcggacc
     8881 gggaaccgga ccgacagatc ctgcacgtcg agcaaccatc cgggggcaga cgctgcgacg
     8941 accggcgtcg ccaccgcggc ggaccggctc gtcgggccgt catcctctgc ggcctcggcc
     9001 gagccgggca cgacatgcag ccagcagcgc acttcgcgcg gaggcaacgc agcgggcggg
     9061 gccagcgaca gcaaggcacc ggccggccgc agcagcaccg gcacctcggc gcgacaacga
     9121 tcgaacaccc gatcgcagcg gggcgcgaat cggcagccgg tgaaaggctg cgacagcgca
     9181 ggcaccgtgc cggtgatcgc cgcgagcacc tcgccgcgct tgtgggcacc gggcagcgac
     9241 tgcagcagca ggcgcgcata cggatgcttg ggatcgccga agaattccgc cgcggcggcc
     9301 acttcgacga tctgccccgc gtacatcagc gccacccgct gggccatgcc cgacaccacg
     9361 gcgaggtcgt gcgtgatcag cagcatcccc atgccctgct cccgccgcaa ctgcttcagc
     9421 aacgcaagga tctgggcctg gatcgcgacg tcgagcgcgg tggtcggctc gtcggcgacc
     9481 aggaacgacg gttcggcggc cagcgcgatc gcgatcatca cgcgctgctt ctggccaccg
     9541 ctcatgcgga acgggtagtc gtcgatacgc cgttcgggct cggggatgcc gacgcggccc
     9601 agccactcga tggccttcgc gcgtgccacc gcaccgcgca acgcggtgtg ggtctcgatc
     9661 gcctcgacga tctggtcgcc gatgcgcatc acgggattca ggctggtcgc cggttcctgg
     9721 aagatcatgc ccaccttgcc gccgcgcagc cggcgcatgg ccgactcggg cagcgacagc
     9781 gcttcctggc cgtcgatctc gacccgaccc tcggtgacgc gtgcgttgtc gggcagcagg
     9841 cgcatcagtg ccagcgctgt gacgctcttg ccgcatcccg actcaccaac cagcgcaaag
     9901 gtctcgccgc gctcgatgca cagcgacagg ccgtcgatcg cccggatcat gcccgcgtcg
     9961 acatcgatcg ccacctgaag gtcggtgatg ttcagcatcg cgtgtcagcc cgccttgccg
    10021 gtcgccgggg cacgcctggc gcgatacacc cgagcccgcg gatcgaacgc gtcgcgcacg
    10081 ccgtcggcga agaaattggc cgccagcacc accgtcacca tgaagccgaa cgcggcggcg
    10141 aacgaccacc acaccagtgg gtcgcggctc atctcgctgc gagccaggtt gatcatcccg
    10201 ccgaagctgt tcatcgacgg gtcgacgccg actccgacgt aggacagcac ggcctcgtag
    10261 aggatgagcc ccgagaaatc gagcactgtc gtgatcagga ccaggtgcat cacgttgggc
    10321 agcaggtgcc gcgccatgat ccgccacggg ccgacaccga acgccgtggc agcctgcacg
    10381 tattcgagtt cgcgcagctt cagtgcctcg gcacgcagca gccggcacag acccgcccat
    10441 ccggtcaccc cgaggatcgc gcacagcagg aacagcttca ggtcggcccg ctcggcaccg
    10501 gtctcgaaca tctcggggtg ctggtcgaga aagacctgca ccatcagcac gcaggcggcg
    10561 atcagcagca cgttgggcac cgacgagagc acggtgtaaa ggtactggat cgcctcgtcg
    10621 acccagccct tgaagtagcc ggccatcagg cccagaccga cggccagagg cagcgtggcg
    10681 acggtggcca gcgtgccgat gacgaacgcg gtgcgtatgc ttttcagggc ctggtagagg
    10741 acatcgttgc cggtgcggtc ggtgcccagc aggtggtagt cgttcatcag cagcgcggca
    10801 ggcccgacga gcaggcacag gaccgtcagc atcatcagcg cggcgcgcca gcggatgtcg
    10861 gtctcgtctc gaagcacgcg gccgagcgcc tcgccccagg gccagccgtt tcggcgagcg
    10921 actccgagca cgatccacgc gcccagcagc agcgccgcac cggcaccgat cgccagtccg
    10981 gccgcgatgc gagcaccgat gtcgcccgcc cactgacgct gagggtcacg caaatgggca
    11041 cccccgtgca gcaggcgtgg cggcaagcgg cgcacctccc cgtcgacggt cagcgattcg
    11101 agcgtgaaac tctggtaggc gagtggtcgc gagtaggtgg cttcgcgcga ctcgatctgg
    11161 cgcgacagca gcgagtcgag ggccgattgc gttcgcgtgt cgtaggcctt cgccgcagtc
    11221 ggcgagcctg tgctcgctgc cggcaaggcg cgccggaagt gaatgctgtc cagcagcgtc
    11281 agcaccaggc acaggccgag cacgatcgcc gacgcgaagg ccgcggcgtc gcggaacacc
    11341 agcgtccagc ttgcgcgcag gttcgggctg cgccagacga cccgcgcgta gacgagcagc
    11401 gcgacaacca gcgcccacat caccgcgtcg gtccacagca gaacgaactt cggcagcatg
    11461 gtgctcatgt cagtgtgtcc accgcacttc gggcgaccca cgacccttga gatgggcgtg
    11521 cgaagtttgc gcgcgtgcct gagccgctgg gtacctccct ccgcgaatgt cccccggctt
    11581 cgcctcctcc tttatttggc tgcgggagat acccagcgtc cccggcacgg gtgacggcgt
    11641 cttgtgtctt gta
//
LOCUS       DAOORV010000110        11606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6920_length_11606_cov_9.764436, whole genome shotgun sequence.
ACCESSION   DAOORV010000110 DAOORV010000000
VERSION     DAOORV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6920_length_11606_cov_9.764436"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..573)
                     /locus_tag="PKV56_13390"
     CDS             complement(226..573)
                     /locus_tag="PKV56_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HOX68852.1"
                     /translation="MELLKVSALFALTAVMEIVGCYLPWLVLRQGKPVWLLLPAAVAL
                     AVFAWLLTLHPAAAGRTYAAYGGMYIVMALAWLYVVDGVALTRWDWAGAALALAGMAI
                     IALQPSTLDPASR"
     gene            complement(573..995)
                     /gene="merR"
                     /locus_tag="PKV56_13395"
     CDS             complement(573..995)
                     /gene="merR"
                     /locus_tag="PKV56_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HOX68853.1"
                     /translation="MPKVLSPADLALTIGALATEAGVNVETIRFYQRRGLLPEPERPP
                     RGVRRYGPADVARVRFIKSAQRIGFSLDEVAQLLQLEDGTGCGQAREIATHRLLDVRQ
                     RLTDLQRIEAALSALVARCADARGRVACPLIAALQEAA"
     gene            1038..1415
                     /gene="merT"
                     /locus_tag="PKV56_13400"
     CDS             1038..1415
                     /gene="merT"
                     /locus_tag="PKV56_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HOX68854.1"
                     /translation="MGNRSPASTPTSSGSERGPLLAGALAALLATSCCLGPLVLLSLG
                     VSGAWIGNLTALEPWRPLFIAVALAALALAARRIWRRVPDCAPGDVCAVPRVRRRYQG
                     LFFAVALLVLLALAFPWFAPLFY"
     gene            1425..1709
                     /gene="merP"
                     /locus_tag="PKV56_13405"
     CDS             1425..1709
                     /gene="merP"
                     /locus_tag="PKV56_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HOX68855.1"
                     /translation="MNKFLVLALGATLAVPAWSAPRTVTLSLPTMDCPVCPITVKKAL
                     GKVEGVNRAEVDFGKRQAVVQFDDAKTSVDALIRATTDAGYPSTVVGGAK"
     gene            1706..1945
                     /locus_tag="PKV56_13410"
     CDS             1706..1945
                     /locus_tag="PKV56_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HOX68856.1"
                     /translation="MSTVELVSTLTCPQCGHATVETMPTDACQWFYECEQCHTVLRPL
                     AGDCCVYCSYGSVPCPPIQQHRLQGEAGRPGACCG"
     gene            complement(2321..4183)
                     /locus_tag="PKV56_13415"
     CDS             complement(2321..4183)
                     /locus_tag="PKV56_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX68857.1"
                     /translation="MNNTEALPIRLPVSCNQLIFQQAPVRFDSWGPINFFVGPNGSGK
                     TTFFNSVMEQARSAFPNRVKILGTGRLGPLEKSVAQWIGDPTSRLFQEESIENVYNTL
                     FSGQDTAHQAFQVLEKRLDLQIRVLGFLRHVFKRSLQFKSTRRGLMVLGTSATGSEYQ
                     IIDECHGLKELITILTFLYDDTFAVLGIDEPELHLHPQFQRFLIDELKAVAGDPSMPG
                     KKLIFLVTHSPILLELRSLADLASIVVFSPGTPPRRATPDSFDAEDQLKMRQALPSFH
                     AAQRELLFSNTPVVVEGPFDAAVLMNVASRLELPLGAAGIGLTTMGGKYQLRAFRALT
                     AALGKPQTRFILDLDAAIDAKALSCLDADPRVTRFLAERGLGDRTLTKLIGELITLLR
                     DYVRDAAVAGLPVAAIKDLTAPTEAELAVALRVLRKARDDGTVQFESGRADAVLGKVD
                     LVRELAASANVLILSRGSIEAYYASPPPPRPSDFEKQQALQAELDAIWATGEAAKLSQ
                     RYEEVVDFVREAGLLHIPLATLTREPIANLVHLLQSEIAAGRITCLEDARVSRAKADG
                     FWHICELTHLNVTSRRTFSGEIRVKRELGGEVIPFDNNTRAYELTSVLPVEPAR"
     gene            complement(4534..6153)
                     /locus_tag="PKV56_13420"
     CDS             complement(4534..6153)
                     /locus_tag="PKV56_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68858.1"
                     /translation="MPAASSDAATKVNLMSGDAYRESLRRYKPKVFVDGREVASVVDD
                     PSLRPGVNALAFTYDYALRAELEPIALATQTRRNRVVSRMLHVNESAGDLLNKLEAVR
                     VLCQETGCAQRYLAHDALNGIAQATARIDDAKGSTEHRGRFEAFLAHVQDNDLSLGIA
                     MTDAKGDRSRKPHQQANADTYVHVVERSAKGIVISGTKAIVTGGPYMHEFLVMPSRNM
                     GREDADFAVCCAVRVDEPGITIASRPAGRPGEKVEHGEALFSRKYGQSTAVVIFDRVF
                     VPWNRVFFAGEWEHSHVLTYSYATHHRHSCIAARAGFGDLLIGAGALMCEANGLDPSK
                     KSNLRDPMVELITITESFFACGVAASVYAEKDQNSGSFMPEPVFSNIGKLLLATKIYD
                     MHRIAHEVSGGLIVALPGPDEDHNPATAAKLSEVLQAAPDIPYDKRIQTARFIEDLTA
                     SYQGGWYSVISLHGGGSPAAMKQEIWRNYPVGSKVELVERLLERGVLHDEKRAITKNR
                     QPGKCCDTGCTTPGQPVMVALPESRRVGVAD"
     gene            6232..6921
                     /locus_tag="PKV56_13425"
     CDS             6232..6921
                     /locus_tag="PKV56_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX68859.1"
                     /translation="MATSTTTSRRPAARKAAAQPRAMLKQALADVRRRAVLDAARSAF
                     FDLGMEKTSMREIAQRAGYTVGAIYSYFASKEEVYGALLSESLERLNAQVQAALDGRT
                     GAVEHVRLAATAFFDFYRENPRDLDLGFYLFQGMNPRGLTPELNEKLNARLRDALQPI
                     QDALQALGLPPKRALEEVTALFAHTVGLLVLSHTGRIRMFKQASQDLFDTYLRQLLGR
                     ASGRGGSAGEA"
     gene            6918..7475
                     /locus_tag="PKV56_13430"
     CDS             6918..7475
                     /locus_tag="PKV56_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOX68860.1"
                     /translation="MNLDAVNARDCVLVLVDYQARLMPAIHDAAAVVANGVLLAQAAH
                     ALDIPVVGTEQNPAGLGPNVEQIRAECQATLAKTHFDACADGLIEALHDAKPMGNQVV
                     VAGCEAHVCMMQTALGLLRAGKRVWVVANASGSRRQADHSAAMARLSQAGATVVTHEM
                     VLFEWLGDCRHPKFRDVLKLIKAAG"
     gene            7818..9515
                     /locus_tag="PKV56_13435"
     CDS             7818..9515
                     /locus_tag="PKV56_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX68861.1"
                     /translation="MEIALSAPPGSPQDPSWPDEAALAAFRAWLQGVPSRAAVDRYLP
                     DRRTAGTSARSVIGRVKRQLVAFAMSRARADLAATLDKASPSDRKLGKCAAAAIEALR
                     GMSLPQPLVGDTVERWLPARLAGVLQAAGIRTLADLTLRVPRRRRWWGGIEGLGPAGA
                     RHIEAFFAQHPALTERARALVTVTQTQELVPWERLVVPEDVDGSRGTFRAPRGSGALD
                     ASNDYQAVNAWLTLHESAATRRAYRKEAERLILWAIVERGRALSSLTTEDAIAYRAFL
                     RHPSPRARWVGAPQPRPSAAWRPFASDLSARSAAYALSVLNALFRWLIEQRYVLANPF
                     AGVKVRGGRPAQLDTSRAFSEHEWKLVRVVADGLEWSYGWSEPAAQRLRFLLDFAFAT
                     GLRISELVGAQLDAIESDAHGDTWIRVVGKGHKAGKVVLPPLARAALDRYLVQRGLPV
                     TPSKWRSSTRLVGSLGEDGSGISTWRLWRVMKRFFSTAADVVVEGTPALAEKLRLATP
                     HWTRHTHATHLLEGGAELTTVRDNLRHASLATTSMYLHTDDARRARQVADRFAVPQA"
     gene            9552..10136
                     /locus_tag="PKV56_13440"
     CDS             9552..10136
                     /locus_tag="PKV56_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68862.1"
                     /translation="MPSISCDACSQLTADHDVVNCGSVEDGYRRLCGRCFNEAAASQL
                     GLQGFEHVAFEPVRMVDGRGAEHEFRFRNWLCGAGLAIDAFESRDGSPAGYRFQVIGE
                     PDDEPLALLGRLIGKMRRALALTHLEDTDHGPQVNDGLVLRGFVDSDPDADHRVPMVV
                     IDGRAISWDEFGRMVATFEGWQFRLEFRDRSEVV"
     gene            10727..10975
                     /locus_tag="PKV56_13445"
     CDS             10727..10975
                     /locus_tag="PKV56_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68863.1"
                     /translation="MNDMSHRNASQAEPMRTGHQSAAHNRWLAAEIQDALDDPRPSLS
                     LEEAMAAMEAEIDAVERRSSIDRAPAEPPLPSKAPRRQ"
     gene            11028..11498
                     /locus_tag="PKV56_13450"
     CDS             11028..11498
                     /locus_tag="PKV56_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68864.1"
                     /translation="MLPGEVKDQGPARGGLVAAQARGAAARARWIEERLVVPGPQLAQ
                     AWGIRLEALATATASGEVVSIAIDGNVYFPKALLSVDRLTAVAICLALRHLRDTERLM
                     FWLRDHGALGGTNPAAALSAGTSIDQVIALAQAWARARTEGGSLMDGRPDSMCA"
BASE COUNT         2033 a         3718 c         3837 g         2018 t
ORIGIN      
        1 aatcgaccca aaccggaagt tcaagcttct ccaaagctgc ccgacgtccg gaccggtcac
       61 ttgcggtcgg ggcgtctgcg aagcagtcct tgatggccaa ccggactggg ggtgcagcag
      121 aaaacttgga ccggttggac ggcagcactg accgaaagct gactgaagac caagtacacg
      181 atcgacggcc acagtgattg cggccgcctg gctggctgac cagagctaac ggctggcggg
      241 atccagcgtc gaaggctgta gcgcaatgat ggccatgcct gccagtgcca atgcggcgcc
      301 ggcccagtcc cagcgcgtca gcgccacgcc gtcgacgacg tacagccaag ccagcgccat
      361 gacgatgtac atgcccccgt aggccgcgta ggtgcgccct gcggcggcag ggtgcagggt
      421 cagcagccac gcaaacaccg ccagcgcgac cgcggcgggc aacagcagcc agaccggctt
      481 gccctgacgc agcaccagcc atggcaggta gcagccgacg atctccatca cggccgtcag
      541 cgcgaacagc gccgacacct tcagcagttc catcatgcgg cctcctgcaa cgcggcgatc
      601 agcgggcatg ccacgcggcc gcgggcatcg gcgcagcggg ccaccagcgc cgacagcgcg
      661 gcttcgatgc gctgcaggtc ggtcagccgt tggcgcacgt cgagcagccg atgggtggca
      721 atctcgcgcg cctggccgca gcccgtgccg tcctcgagct gaagcagctg tgccacctcg
      781 tccaggctga agccgatgcg ctgtgccgac ttgatgaagc gcacgcgggc cacatcggca
      841 ggcccatagc ggcgcacgcc gcgcggcggc cgctcgggtt cgggcaacag gccgcggcgt
      901 tggtagaagc ggatggtctc gacattgacg ccggcttcgg tggccagcgc gccgatggtc
      961 agggcgaggt ctgctgggct aaggactttc ggcatgaggc ttgactccgt actcaagtac
     1021 ggaagtatgc tggacgcatg ggcaaccgtt cccctgcatc cacgccgact tcgtccggca
     1081 gcgaacgtgg cccactgctc gcgggcgcgc tggcggccct gctggcgaca agctgctgcc
     1141 tgggtccact ggtgctgctg agcctgggcg tctccggcgc ctggatcggc aacctgacgg
     1201 cgctggagcc ctggcggccg ctgttcatcg cagtcgcact ggctgcccta gcgctggcgg
     1261 caaggcgcat ctggcggcgc gtgcccgact gtgcgcccgg cgacgtctgt gcggtgccgc
     1321 gcgtgcgccg gcgctaccaa gggctgttct tcgccgtcgc gctgctggtg ctgctggcgc
     1381 tggccttccc gtggttcgcg ccgctcttct actgaaaggt ccccatgaac aagttcctgg
     1441 tattggccct tggtgccaca ctggccgtgc cggcgtggag cgcgccgcgc accgtgacct
     1501 tgtcgctgcc cacgatggac tgcccggtgt gccccatcac cgtcaagaag gcgctgggca
     1561 aggtcgaggg cgtcaaccga gccgaggtgg acttcggcaa gcgccaggcc gtggtgcagt
     1621 tcgacgatgc caagaccagc gtcgatgccc tgatccgggc gaccaccgac gccggctatc
     1681 cgtcgaccgt ggtcgggggc gccaagtgag tacggtcgaa ctcgtgtcga cgctgacctg
     1741 cccgcagtgc ggccatgcga cggtggaaac catgcccacc gacgcctgcc agtggttcta
     1801 cgaatgcgag cagtgccata cggtactcag gccgctggcg ggcgactgct gcgtctactg
     1861 ctcgtatggc agcgttccgt gcccgccgat ccagcagcat cgtctgcagg gtgaggccgg
     1921 aagacctggt gcctgttgcg gctgaccgcc tgtaccaagc ggccatcgac agcgggtgac
     1981 gtcgccgcag ctcaagataa tgactgggtc ggcctgcgcc ggtcgggcac ccgatctcac
     2041 cgccggaaag ccggccccca ggttgaacga tctgatagcc gacatccgcg gtcggcagtg
     2101 gccgacccaa accggccgga cgcgctgctc caaagcggtc ggccggcagg ccatcgctct
     2161 tgcggtctca ccaccatgaa gcggacgttc gtggtctcac tcacccggcc agaagcggac
     2221 cttccacaac ctaccgacct gccacctgcc gcgcagcgcc ctgagaaccg gttgcagaga
     2281 gactccaagt cgtggagcgg caggaatcaa tatgcgacga ctatcttgca ggctccaccg
     2341 gtagcaccga agtcagttca tatgcacgcg tgttgttgtc gaacgggatt acctcgccgc
     2401 caagctcacg cttgactcga atctctccag agaaggtgcg gcgacttgtc acgttcagat
     2461 gggtcagctc gcagatatgc caaaagccgt cagccttggc acgagacact cttgcgtctt
     2521 caagacaagt aatcctgccg gcggcaatct ccgactgcag cagatgaacg agatttgcga
     2581 tgggctcgcg cgtgagcgtt gccaatggga tatgaagcag tccggcttcg cgcacaaaat
     2641 ctactacctc ctcgtagcgt tgggagagtt tcgctgcctc cccggtggcc catatggcgt
     2701 ccagctctgc ctgaagtgcc tgctgcttct cgaagtcgct cggcctaggc ggcggcggac
     2761 tggcgtagta cgcctcgatg gaaccgcggg agagaatgag aacgtttgcc gaagccgcaa
     2821 gctcacgaac gagatcgacc tttccaagca ctgcatcggc gcggccagac tcgaattgaa
     2881 cggtcccgtc gtcgcgcgcc tttcgtagta cgcggagcgc cactgcaagc tcggcctccg
     2941 ttggtgccgt caagtccttg atagccgcaa caggtaaacc agcaaccgca gcgtcacgca
     3001 catagtccct gagcagcgtg atcaactcgc cgatgagttt ggtaagggtc cgatcaccca
     3061 atccacgttc agcaaggaat ctggtcactc gcggatcggc atccaaacaa ctgagagcct
     3121 ttgcatcgat agccgcatcg agatcgagaa taaacctagt ctgtggtttt ccgagtgccg
     3181 ctgtaagggc gcgaaaggct cgaagttggt acttcccgcc catcgtggtc agcccgatgc
     3241 ctgcggcgcc aagtggcagt tccagccgac ttgcgacgtt cattaggaca gccgcgtcga
     3301 acggaccctc gacaacgaca ggcgtattgg agaacagaag ctcacgctgt gccgcatgga
     3361 acgatggcaa cgcctgcctc atcttcagct ggtcttcggc atcgaatgag tcaggcgtcg
     3421 cacgacgcgg cggagtgcct ggcgagaaca ctacgatgct tgccaagtcg gccagcgaac
     3481 gaagctcaag gaggatcggc gagtgagtga caagaaagat cagctttttg cccggcatcg
     3541 acggatcgcc cgcgacagcc ttcagttcat cgatgagaaa gcgttggaac tgtggatgca
     3601 gatgcagttc aggctcgtcg attccgagga cagcaaaagt gtcatcgtag agaaaggtca
     3661 agatagtgat cagttccttg agcccatggc actcgtcgat gatctgatac tcacttcccg
     3721 tagcagacgt gccaagaacc atcagtcctc gtcgtgtgga cttgaactgc agggagcgtt
     3781 tgaaaacatg acgcaagaag cccaacacgc ggatctgcag atcgaggcgc ttctccaaga
     3841 cctgaaacgc ctgatgtgcc gtgtcttgac cagagaagag ggtgttgtag acgttctcga
     3901 tgctctcctc ttgaaagagt cgggacgtgg gatccccgat ccactgcgcc acactcttct
     3961 caagggggcc gagtcggccg gtaccgagaa tcttcacgcg attggggaaa gccgagcggg
     4021 cctgctccat caccgaattg aagaaagtag tctttcctga tccgtttggt cccacgaaga
     4081 aattgatagg accccacgag tcgaaccgga ccggtgcctg ctgaaatatg agctgattgc
     4141 aggaaacggg gaggcggatc ggcagggctt cggtgttatt catgctgctc caagtctcat
     4201 cgaccaatac agcccaactg taatcgcgcg cgactcggat acccgtgacc ggcaaggcac
     4261 acgaagttgg ttgggccgac tggcggccgt tgtgaatgct gggacgactg ccgcggcggt
     4321 cgttgggcgg tgtcgcaagc cttcgtgttt gggtccggac acaggccctg ccggtggacc
     4381 gcttagggtc gaccctcgac ggtcgccgcg ccaggattac cgccgcagag ctgtcgttgc
     4441 cggtgagcta cgagcaagca gcccttcgtg agctgacggc ctcgaccccc agcggaagtt
     4501 cgcggcaatg ggccgacccg cgggagccgt cgttcaatcc gcaacgccga ctcgccgact
     4561 ttcgggcaac gccaccatca ccggttggcc cggcgtggtg cagccggtgt cgcagcactt
     4621 ccctggctgc cggttctttg tgatggctcg cttttcgtca tgcagcacgc cgcgctccag
     4681 cagacgctcc acgagttcca cctttgaccc caccgggtag ttgcgccaga tctcctgctt
     4741 catcgccgcg ggcgagccgc cgccgtgcag actgatcacc gagtaccagc ccccctggta
     4801 ggacgccgtg aggtcctcaa tgaagcgcgc cgtctggatg cgtttgtcgt aggggatgtc
     4861 gggcgccgcc tgcagcacct cggacagctt cgcggccgtg gccgggttgt ggtcctcgtc
     4921 cgggccgggc aacgccacga tcagaccacc tgacacctcg tgcgcgatgc ggtgcatgtc
     4981 gtagatcttg gtcgccagca gcagcttgcc gatgttgctg aacaccggct cgggcatgaa
     5041 cgagccgctg ttctggtcct tctccgcgta gacgctggcc gccacgccac aggcgaaaaa
     5101 gctctcggtg atggtgatca gctcgaccat cgggtcgcgc aggttgctct tcttgctcgg
     5161 gtccagcccg ttggcctcgc acatcagcgc gcccgcgccg atcagcaggt caccgaagcc
     5221 ggcgcgggcg gcgatgcagc tgtgccggtg gtgcgtggcg tagctgtagg tcagcacatg
     5281 gctgtgctcc cactcgccgg cgaagaagac acggttccag ggcacgaaca cgcggtcgaa
     5341 gatcaccacc gcggtcgatt ggccgtactt gcgcgagaac agcgcctcgc catgctcgac
     5401 cttctcgccc ggtcgacctg ccgggcggga cgcgatggtg atgcccggct cgtccacacg
     5461 cactgcgcag cacaccgcga agtcggcgtc ctcgcggccc atgttgcggc tgggcatgac
     5521 gaggaactcg tgcatgtacg ggccgccggt gacgatggcc ttggtgccgc tgatgacgat
     5581 gcccttggca ctgcgctcga cgacatgaac ataggtgtcg gcgttggcct gctgatgcgg
     5641 cttgcgactg cggtcgccct tggcgtcggt catcgcgatg cccagcgaca ggtcgttgtc
     5701 ttgcacatgg gccaggaatg cctcgaaacg gccgcggtgt tcggtgctgc ccttggcgtc
     5761 atcgatgcga gccgtggcct gggcgatgcc gttgagcgcg tcgtgcgcca ggtagcgctg
     5821 cgcgcagccg gtctcctggc acagcacccg cacggcctcc agcttgttca gcaggtcacc
     5881 ggccgactcg ttgacgtgca gcatgcggct gacgacccga ttgcggcggg tctgggtggc
     5941 cagtgcaatc ggctccagtt cagcgcgcag agcgtagtcg taggtgaatg caagggcgtt
     6001 gacaccgggg cgaagcgacg gatcatcgac cacgctggcc acctcgcgac catcgacgaa
     6061 gaccttcggc ttgtagcggc gcagcgactc gcggtaggcg tcgccggaca tcaggttgac
     6121 tttggtggca gcgtcggagg aagcggcagg catggttgtc tccaggcatt gaacattgag
     6181 catctatgtt aaacactgtt caatgtgagt gatcaacgtt tacaatggcc catggccaca
     6241 tcgacgacga cctcccgccg cccggctgcc aggaaggctg cggcccagcc gcgcgcgatg
     6301 ctcaagcagg cgctggccga cgttcggcgc cgcgcggtgc tggacgcggc acgctcggcg
     6361 ttcttcgacc tgggcatgga gaagaccagc atgcgcgaga tcgcgcagcg tgccggctac
     6421 accgtgggcg ccatctacag ctacttcgcc agcaaggaag aggtgtacgg tgccctgctg
     6481 tcggaatcgc tggagcggct gaatgcgcag gtgcaggccg ccctcgacgg ccgcaccggc
     6541 gcagtcgagc atgtgcgact tgcggcaacg gcgttcttcg acttctaccg cgagaacccg
     6601 cgtgacctgg accttggctt ctacctgttc cagggcatga atccccgtgg tctgacgccc
     6661 gagttgaacg agaagctgaa cgcgcgtctt cgtgacgcgc tgcagccgat ccaggatgcc
     6721 ttgcaggcac tggggctgcc cccgaagcgc gcgctggaag aggtgaccgc gctgttcgcg
     6781 cacacggtcg gcctgctcgt gctcagccac acgggccgga tccggatgtt caagcaggcg
     6841 tcgcaggacc tcttcgacac ctaccttcgg caactgctgg gccgcgcgtc gggccgcgga
     6901 ggaagcgcag gggaggcatg aacttggacg ccgtgaacgc gagggactgc gtgctggtgc
     6961 tggtggacta ccaagcccga ctgatgccgg ccattcacga cgctgctgca gtggttgcta
     7021 acggcgtcct gttggcgcag gcggcccatg cgctggacat tcctgtcgtc gggaccgaac
     7081 agaacccggc gggcctcggt ccgaacgtcg agcagatccg ggccgaatgc caggccacgc
     7141 tggccaagac gcatttcgat gcctgtgcag atggactgat cgaagcactg catgatgcga
     7201 agccgatggg caatcaggtc gtcgtcgctg gatgcgaggc ccacgtctgc atgatgcaga
     7261 cggcgctggg cttgctgcgt gcgggcaagc gcgtctgggt ggttgcgaat gcgagcggtt
     7321 cgcgacgtca ggcggatcac tcagcggcca tggctcggct atctcaggcg ggcgccacgg
     7381 tcgtgacgca cgagatggtg ctgtttgagt ggcttggcga ttgccggcac ccgaagttcc
     7441 gggatgtcct gaagctcatc aaggccgctg gctgattgag ccgggcgacc ggaagcgggg
     7501 taaggggaac accactggcg tcaggccgct tctggccagg tctagccgaa gcggctctca
     7561 agttcgtcgc cggaaagctg tcgttcagcg gcacgccgcg gccagatttc agagctcagg
     7621 ccgaccggcc gcttgtggcc gatggctggt ctccgcgagc ggcagcttac tggagcctca
     7681 atttgggctg cgcttgcccc cgggaagccg agactctcac cgcggtgaga gttcgctggc
     7741 agacgtgctc ttcggcagtg ctaccaagat aaacaacatt agcttggtag gcacgcggtc
     7801 gatctaaact ggcatggatg gaaatcgcgc tgtcagcgcc gccgggctcg ccccaagacc
     7861 catcctggcc ggacgaggct gcgctggccg cattccgcgc ctggcttcaa ggggttccca
     7921 gccgggccgc cgtggatcgc tatctgcccg atcgccggac cgccggcaca tcggcgcgct
     7981 cggtgatcgg gcgggtcaag cgccagctcg tggccttcgc gatgagccgc gcacgggccg
     8041 atctggccgc cacgcttgac aaggccagtc cgtccgaccg gaaactcggc aagtgtgccg
     8101 cggccgccat cgaggcactg cgcggcatgt cgctgccgca gcccctggtt ggcgacaccg
     8161 tcgagcgctg gctacctgcg cgactggcgg gtgtgctgca agctgcgggc atccgcacgc
     8221 tggctgactt gaccctgcgc gtaccgaggc gccgacggtg gtggggcggc atcgagggac
     8281 tcgggccggc cggcgcccgc cacatcgagg cgttcttcgc ccagcatccc gcgctgactg
     8341 aacgcgctcg tgcgctggtg actgtgacgc agacccagga actggtcccc tgggagcggc
     8401 tggtcgttcc ggaagacgtc gatggctcgc ggggcacgtt ccgggcgcca cggggcagtg
     8461 gagcgctcga cgccagcaac gactaccagg cggtgaacgc ctggctgaca ctgcatgagt
     8521 cggctgcgac ccggcgtgcc taccgcaagg aggccgaacg gttgattctg tgggcgatcg
     8581 tcgagcgagg gcgagctctg tcgtcgctga ccaccgagga cgccattgcc taccgtgcct
     8641 tcttgcgcca cccgagccct cgcgcgcgct gggtcggcgc accgcagccg cggccatcag
     8701 cggcatggcg ccccttcgcc agcgacctct cggcacgctc ggccgcctac gcgctgtcgg
     8761 tactcaacgc cctgttccgc tggctcatcg agcagcgcta cgtactggcc aacccgttcg
     8821 cgggcgtaaa ggtgcgcggc ggccggccgg cccagctcga cacgtcccgc gccttcagcg
     8881 agcacgagtg gaagcttgtc cgggtcgtcg ccgacggcct ggagtggtct tacggctgga
     8941 gcgagccggc cgcgcagcgg ctgcgcttcc tgctcgactt tgccttcgcc acgggccttc
     9001 ggatcagcga actcgtgggc gcgcagctcg acgcaatcga atccgatgcg cacggcgaca
     9061 cctggatccg ggtcgtcggc aagggccaca aggccggcaa ggtcgtgctg ccgccattgg
     9121 cccgcgctgc gctggatcgc tacctcgttc aacgggggtt gccggtcacg ccctcgaagt
     9181 ggcgctcgtc tacacggctg gttggcagcc tcggcgaaga tggaagcggg atctccacgt
     9241 ggcgcctgtg gcgggtgatg aaacggttct tcagcaccgc cgctgacgtc gtggtggaag
     9301 gcactccggc gctggccgag aagctgcgct tggccacacc gcactggacg aggcacacgc
     9361 atgccacgca cctgctcgaa ggcggcgccg agctgaccac ggtgcgagac aacctgcggc
     9421 acgcctcact tgcaacgacc tcgatgtacc tgcacaccga cgatgcgcgg cgtgccaggc
     9481 aagtggccga tcggttcgcc gttccacagg cgtagcctac agttgactcg ccttcacaca
     9541 ccctgcgacc catgccctcc atctcctgcg atgcctgcag ccagctcacg gccgaccacg
     9601 atgtggtgaa ctgcgggtcg gtggaggacg gataccggcg gctctgcgga cgatgcttca
     9661 acgaggccgc cgccagccaa cttgggctgc aggggttcga gcacgtggcc ttcgagcccg
     9721 tccgtatggt cgatgggcgc ggggcggagc acgagttccg gtttcgcaac tggctgtgtg
     9781 gcgccggctt ggcgatcgac gcgttcgagt cgcgcgatgg cagcccggcc ggatatcggt
     9841 ttcaggtgat cggggagcct gacgacgagc ccctggcgtt gctgggccgg ctcatcggca
     9901 agatgcgccg ggccttggcg ctcacgcatc tggaggacac cgaccacgga ccgcaggtga
     9961 acgatggctt ggtgctgcgg gggttcgtcg acagcgaccc cgacgcggac catcgtgtgc
    10021 cgatggttgt catcgatggc cgcgcgatct cctgggacga gttcggccgc atggtggcga
    10081 ccttcgaggg ctggcagttc aggctggagt ttcgcgatcg gagcgaagtg gtctgatcgg
    10141 gccggtcgcg tgagaccgta gggtccggcc tcgcaagggt gcctgagacg aagcgcacca
    10201 atctgcacga aaagcccgcc gcgcccggaa gagccggaat cgtaagtgct tgtcgtgcaa
    10261 cgagttctca gagcaaagcg caccaatctg cacgaaaagt acgactaccc cctcagggag
    10321 aagccacagg cgacgttcga actgcgtcag gcgcgttaag gcgtgcgttc gagcgatcgg
    10381 tactcggtac aacgaatgcg gaagttgaca taaacattaa agtcgctcat tcggcctgct
    10441 tgcgaccatt gcgccgaagt gacgcaaggt ggcggttgcg tctgcggctc tctctggcca
    10501 aagacctcaa tgccaccgtt gtcgtgtgcc gtgccgcctc cctggcggag gcgtcgaatc
    10561 gtagggtcac gagttccgtg gcaggctggg acgactggcg atccgagcca gcattcttac
    10621 tgaatcccgc ccacgctgca catggccgtt gcgccgcgtc agtcgaatgc gcatgatgct
    10681 tctatcgtga gtgcggcggc gccgtcgcca agacttgacc atctccgtga acgacatgag
    10741 ccaccgcaac gccagccagg ccgaaccgat gcgaaccggc catcaatccg cagctcacaa
    10801 ccgatggctc gccgccgaaa tccaagacgc gctggacgat ccgcggccaa gcctctctct
    10861 cgaagaagcc atggccgcca tggaagctga gatcgacgcc gttgagcgca ggtcctccat
    10921 cgatcgcgct ccggctgaac ctcctttgcc ttccaaagcc ccgcgtcgac agtgaacgtc
    10981 agcatgttct actcctggtg aggttccctg caggaggttt gcgcacgatg ttgcctgggg
    11041 aggtcaagga tcaagggccg gcacgaggcg gcctggtcgc tgctcaggcc cgcggcgcag
    11101 cggcgcgcgc ccggtggatc gaggaaagac tcgtcgtgcc cggtccgcaa ttggcacagg
    11161 cgtgggggat ccggctggag gccctggcga cggccaccgc aagcggcgag gtggtcagca
    11221 tcgccatcga cggtaacgtg tacttcccca aggcgttgct gagcgtagat cgtttgaccg
    11281 cggtcgcgat ctgcctcgcg ctgcggcacc tccgagatac cgagaggctg atgttctggc
    11341 tgcgcgacca cggagccctt ggcggcacaa acccggccgc ggccttgagc gcgggaactt
    11401 cgatcgacca ggtgatcgca cttgcacagg cctgggcgcg tgcgaggacg gagggcggca
    11461 gcctgatgga tgggaggcca gactctatgt gcgcttgacc gacttgacgg gctccaaaga
    11521 gatgggttcc acgtcctttc gcagcagttc gtcaatcacc gccgcatgcc gcgccagatg
    11581 ctccggcgcc aggtgcgcat accgct
//
LOCUS       DAOORV010000111        11539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_6972_length_11539_cov_7.315657, whole genome shotgun sequence.
ACCESSION   DAOORV010000111 DAOORV010000000
VERSION     DAOORV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_11539_cov_7.315657"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="PKV56_13455"
     tRNA            complement(1..72)
                     /locus_tag="PKV56_13455"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Val,seq:tac)
     gene            complement(122..415)
                     /locus_tag="PKV56_13460"
     CDS             complement(122..415)
                     /locus_tag="PKV56_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOX68865.1"
                     /translation="MHLRGGHFVNKSELIEHIAKQSDISKAAAGRALEALIGGVKSTL
                     KKNNSVTLVGFGTFSVTKRAARSGRNPRTGAAIKIKAAKVPKFRPGKALKDAV"
     gene            complement(530..1630)
                     /locus_tag="PKV56_13465"
     CDS             complement(530..1630)
                     /locus_tag="PKV56_13465"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HOX68866.1"
                     /translation="MNRHRIAVIAGDGIGKEVMPEGLRALEAAADRHGIWLQIDHFDF
                     SSWEYFERHGQMLPDDWKQQIGGHDAIFFGAVGWPARIADHVSLWGSLLRFRREFDQY
                     VNLRPARLMPGIRSPLVNRSGEPLRPGEIDMMIVRENTEGEYSSIGGRMYEGTEREIV
                     MQQTVMSRVGVDRVLKFAFDLAASRPRRKLTSATKSNGIAITMPYWDERVAAMAANYP
                     SVEVDQFHIDILAAHFVQRPQQFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPA
                     RTFPSLFEPVHGSAPDIAGRGIANPIGQIWSAALMLDFLGHREAHDAIVVAIEQVLDP
                     ASGAPRTPDIGGDASTIDVGRAVEQAIRVRSV"
     gene            complement(1627..2523)
                     /locus_tag="PKV56_13470"
     CDS             complement(1627..2523)
                     /locus_tag="PKV56_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX68867.1"
                     /translation="MPAVFVMIWSTGFIVARYAMSNSPTFTFLSMRFGLSIVCFGVWI
                     AASRAPWPVGRVQWLHLGVTGVLMHAAYLGGVWAAVKAGIGAGTVALLVGLQPVLTAL
                     WLSAAVAPDRAHAVTARHWAGLGLGLLGLTLVVWRKLGVGEITPWNLGLATVALIGIT
                     AGTLYQKRFVAPCDVRTANTVQLLAAMIVSLPLALLESEPIHWNPTFLTAMAWSVVVL
                     TIGGSSLLYLLIQRGAATRVTSLLYLVPPMTALLAWLLFDEVLTASMLVGMVLTAVGV
                     ALVVSVPSNQSARPGAGTGDPT"
     gene            complement(2578..3144)
                     /gene="pgsA"
                     /locus_tag="PKV56_13475"
     CDS             complement(2578..3144)
                     /gene="pgsA"
                     /locus_tag="PKV56_13475"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOX68868.1"
                     /translation="MFFNLPTLLTWARIVAIPLVVGVFYLDLNDVLRNTIATALFIVV
                     ALTDWADGYLARRLNQTSSFGAFLDPVADKFLVCAALLVLVHLNRLHALVALVIIGRE
                     IAISALREWMAQIGASRSVAVHMLGKLKTMVQMIAIPFLLYHGMLFGVIDTHWWGTLL
                     IYISAVLTVWSMVYYLQKALPEIRAKAR"
     gene            complement(3194..3640)
                     /locus_tag="PKV56_13480"
     CDS             complement(3194..3640)
                     /locus_tag="PKV56_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68869.1"
                     /translation="MSTGRPVPVAVPVAPVERPAAPAVAAGAAAAVRLPAPATVRSAN
                     ELHLQAARRIAAANPHLIYNDRPPDVLLAIPVLAVDLNADGSIRHIDVLRYPRQAKDT
                     TQIAIDAVRRAAPFGDVSHLPRPWRFTETFLFNDDRRFKPRTLNDR"
     gene            complement(3756..5777)
                     /gene="uvrC"
                     /locus_tag="PKV56_13485"
     CDS             complement(3756..5777)
                     /gene="uvrC"
                     /locus_tag="PKV56_13485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOX68870.1"
                     /translation="MSETPDSAGATAAEPDERRQRLLADVAALPGLPGVYRYFDAQGG
                     VLYVGKARDLKKRVSSYFQKSHGGTRIGHMITKIVRMETTVVRSEAEALLLENNLIKA
                     LNPRYNILFRDDKSYPYLKITGTPGNASGSLASQYPRMAYYRGPVDQRHRYFGPYPSA
                     WAVKESIQLLQKVFRLRTCEDTVFNNRSRPCLLHQIKRCSGPCVKLIPADDYARDVAN
                     AERFLQGEQQQVMDRLQAQMMVHAEALQFEQAAEIRNQIGALSRVLHQQSVEDNTGIA
                     TRDVDILAVKVQGGRACVNLAMVRGGRHLGDRPFFPVHIGDATALDVGPTADGPGRDE
                     AASAPEALVLEAFIAQHYLDGGVPPLLVLNHPVDRALIDALSAQSGVKVTAQHQPRDA
                     RRAWLDMCIKGAELRLAQLLAEEGSQQARTRALVDALALEVEQLDTFRIECFDISHTA
                     GEATQASCVVFENHKMQSAQYRRYNIEGITGGDDYAAMRQVLMRRYGRLAAQVARQVE
                     EAGEPADESVFARPELRLPDLVLVDGGRGQVSMAREVFEELGLDLGLIAGVEKGEGRK
                     VGLEELVFADGRDKVYLGRDSAALMLIAQIRDEAHRFAITGMRAKRASVRTGGSKLED
                     IPGVGPKKRAKLLQRFGGVRGVASASVTDLLTVDGISKDLAEEIYRALH"
     gene            5814..6995
                     /gene="earP"
                     /locus_tag="PKV56_13490"
     CDS             5814..6995
                     /gene="earP"
                     /locus_tag="PKV56_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOX68871.1"
                     /translation="MSLPCSQTPGIGRTWDIFCRVVDNHGDAGVCWRLATDLASRQID
                     VRLWIDDARALAWMAPTGRHGVRVLAWPDGDQDISHALDPAPSVVVETFGCGLPSPYL
                     QVMARARGQAQAPVWINLEYLTAEPYAQRSHCLPSPQSSGPARGMNSWFFYPGFTTGT
                     GGLLRETDLVARQVAFDRMAWLATHGIAPRADERVVSLFCYANPNVPDLLQALAARPT
                     LLLTTPGHATEQVLQALSAAELPARLRIQALPWLTQIDYDHLLWSCDLNFVRGEDSLV
                     RAIWAGRPFVWQIYPQDDSAHEAKLDAFIAQKLATVQPAWGEAIRRLSWQWNSLVGST
                     GSQAGDGIDEWNLLNTPQGMSAWTSRCAAWRDELIELPDLTSQLLRFVAGPQSREDAK
                     I"
     gene            7061..7615
                     /gene="efp"
                     /locus_tag="PKV56_13495"
     CDS             7061..7615
                     /gene="efp"
                     /locus_tag="PKV56_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOX68872.1"
                     /translation="MKIAQEIRAGNVIMHGKDPMVVLKTEYSRGGRNSATVRMKLKSL
                     LSNSGTEVVFKADDKMDQVILDKKECTYSYFADPMYAFMDAEYNQFEVESENMGDAVQ
                     YLEDAMPVEVVFYDGKAISVELPTSLVREIVETEPAVKGDTSGKVLKNAKLATGFEIS
                     VPLFVQTGDKVEIDTRTHEYRKRV"
     gene            7703..8653
                     /locus_tag="PKV56_13500"
     CDS             7703..8653
                     /locus_tag="PKV56_13500"
                     /inference="COORDINATES: protein motif:HMM:NF023354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HOX68873.1"
                     /translation="MAFDFNAVSAPFRMQPGMRRLAPGTTQLTPILPGQQVFSEKLEV
                     LRLHPGQALLTSPGFDSGPALAALFQQGATEHPQAWRVAPDGSVHALHLGWSVQGDQA
                     TSMDNDAPQEIGDCLAALPPEWRTTALFCLAFAEDFAVICGDTSIIPWLAVCLPSHWA
                     PEEKIGRHFAEVHAPVADNHLLITASDHLARLVTGTDRWQRQVWTLTRHPSLHAHPAR
                     LDAADWPAEADADQLAAQAWLRTEHQTFIPLASPRQAVFTIHVEVRPLALAITEPAQA
                     RRLHDALETMTASVLAYRSLTTARDRLLSWLHARSMEGAG"
     gene            8650..10530
                     /gene="mnmC"
                     /locus_tag="PKV56_13505"
     CDS             8650..10530
                     /gene="mnmC"
                     /locus_tag="PKV56_13505"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HOX68874.1"
                     /translation="MSDPVAAGQCKDATACAAVLTELWKDDDRFIVLDDRFDGGERFQ
                     SLLQAWRDDPCRCAQLHVITASPEIVDGCTSSALREEIADRWPPITANMHRLAFDGGR
                     VQWMLVPTPMQAALRTLVAAVDVFACSAPPAADPYATRRWAKALARLAGADARLLFDA
                     GSVCDSMRHALRSAGFAEQPDTDPADRRGADIERWRHAPQFTSRQPPARSGAGARSRD
                     PVLIVGAGLAGCAMAWALAEQGRESFVFERRATVALETSGNPAGLFHGIVNPQDGAHA
                     RFNRGAALEAQRAVAIALAQHQVQGSARGLLRLGNALGNDDLPTMRATLARLGLPGDY
                     VLAVSAQQASELSGLALRQAAWWYPGGGWVQPAGLARSFLERAGPRVALRADSSVHSL
                     TRESGLWQLRDERGSILAAADVVVLANAGDALRLLQPWMPASQPHFWPLLRVRGQITT
                     LPVAAIGALGLPRVPIAGAGYLLPGVDGLAIFGATSQADDDDPAVRDSDHRLNLDQLS
                     RLTGIDHRIEPTAHGIDGRTGWRWSTDDRLPVLGAVPDWTAALGARRLEQPVHVPRLQ
                     GLYACTAFGSRGITWAALAAQVVAASITGAPMPIEAGLLDSVDAARWVVRAATRRTRR
                     VE"
     gene            complement(10551..10739)
                     /locus_tag="PKV56_13510"
     CDS             complement(10551..10739)
                     /locus_tag="PKV56_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68875.1"
                     /translation="MAKPNYSFEKRQRELAKKKKKEEKVKEKSERKQGGEGGTESASD
                     EAAGASPGAGADSGSPSA"
     gene            complement(10813..11487)
                     /gene="rpiA"
                     /locus_tag="PKV56_13515"
     CDS             complement(10813..11487)
                     /gene="rpiA"
                     /locus_tag="PKV56_13515"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOX68876.1"
                     /translation="MTQDELKAMVGQAALAHVAPGSVVGVGTGSTVNCFIDALAGIRE
                     RIVGAVSSSEKSSERLRAHGIAVLDSNAIDRIPVYIDGADEIDPNGHMIKGGGAALTR
                     EKIVADLADRFVCIADVSKRVPVLGRFPLPVEVIPMAASQIARRFTAFGGRAVLRAGV
                     VTDNGCQLLDVHGLAIADPLAMEQEINQWPGVVTVGIFARHRASLCLFGTPDGVETVD
                     YGAPRR"
BASE COUNT         2025 a         3898 c         3708 g         1908 t
ORIGIN      
        1 gggtgctgac gggttcgaac cgccgaccta cgccttgtaa gggcgccgct ctaccaactg
       61 agctaagcac ccggccatcg agagctgccg acgcggcacg accggccgcg cgggagaggg
      121 ttcagacagc gtccttcagc gccttgcctg gacggaactt cggcaccttg gcggccttga
      181 tcttgatcgc cgcgccggtg cgcgggttgc ggccgctgcg agcagcgcgc ttggtcacgc
      241 tgaacgtacc aaaaccgacg agcgtcacgc tgttgttctt cttgagcgtt gacttcacac
      301 caccgatcag cgcttcaagc gcgcgacctg cggcagcttt cgaaatgtct gattgcttgg
      361 cgatgtgttc gatcagttct gacttgttca caaagtgtcc ccctcgaaga tgaatgcgtg
      421 ttgtttggtt cgctgataac cgtgagcctc ggagagccac ggccctcgcc ggattacatc
      481 cgcgaggtag gtgggtgtca agcgaggaaa ggcattctat cgatctgttt cacacggacc
      541 gcacgcggat cgcctgctcg acggcccgac ccacatcgat ggtcgatgcg tcaccgccga
      601 tgtctggcgt tcgtggcgca ccagaagcgg gatcgagcac ctgttcgatc gcaacgacga
      661 tcgcgtcgtg cgcctcgcga tgacccagga aatcgagcat cagcgccgca gaccagatct
      721 gtccgatcgg gttcgcgata ccgcgaccgg cgatgtcagg ggcagaaccg tgcacaggct
      781 cgaacagcga gggaaacgtg cgcgccgggt tcagattggc cgatggcgcg atgccgatcg
      841 tgcccgtgca ggccggaccc aggtcggaca ggatgtcccc gaacaggttg ctcgcgacca
      901 ccacgtcgaa ctgctgcggg cgctgcacga aatgcgcagc aaggatgtcg atgtggaact
      961 ggtcgacctc caccgacggg tagttcgcgg ccatcgccgc gactcgttcg tcccagtaag
     1021 gcatcgtgat cgcgatgcca ttggacttgg tggccgaagt cagcttgcgg cgcggacgtg
     1081 aggccgccag gtcgaatgcg aacttcagca cccgatcgac accgacgcgc gacatcaccg
     1141 tctgctgcat gacgatctcg cgttcggtac cttcgtacat gcgccctccg atgctcgagt
     1201 actcgccctc ggtgttctcg cgcacgatca tcatgtcgat ctcgccgggt cgcagcggtt
     1261 caccggaccg gttgaccaac ggcgatcgga tgccaggcat caggcgcgca ggtcgcaggt
     1321 tcacgtactg gtcgaactcg cgccggaatc gcagcagcga accccacagc gacacgtggt
     1381 cggcgatcct ggcgggccag ccgacggccc cgaagaagat cgcatcgtgt ccaccgatct
     1441 gttgcttcca gtcgtcgggc agcatctggc cgtgacgctc gaagtactcc cagctggaga
     1501 aatcgaagtg atcgatctgc agccagatgc catgccggtc ggcagcggcc tcgagcgcgc
     1561 gcaagccctc gggcatcact tccttgccga tgccgtcgcc tgcgatgacg gcgattcgat
     1621 gtcgattcac gttgggtctc ctgttccggc gccaggccgc gcagactggt tcgatggcac
     1681 gctgacgaca agggccacgc cgactgcagt cagcaccatg ccgaccagca tcgatgccgt
     1741 cagcacctcg tcgaacagca gccaggccag cagcgcggtc atgggaggca caaggtacag
     1801 caggctggtc acacgggtcg cagcgccccg ctggatcagc aggtaaagca gcgaactgcc
     1861 gccgatcgtc agcacgacga ccgaccaggc catggcagtc aggaaggtcg ggttccagtg
     1921 gatcggttcg gattcgagaa gcgccagcgg caggctgacg atcatcgctg caagcagttg
     1981 aaccgtgttc gcggtgcgca catcgcacgg cgcgacaaac cgcttctggt agagcgttcc
     2041 ggcggtgatg ccgatcagcg cgaccgtcgc cagacccaga ttccacggag tgatctcgcc
     2101 gacaccgagc ttgcgccaca ccaccagcgt caacccgagc aggccgagac ccagccctgc
     2161 ccaatgccgc gcggtcaccg catgcgctcg gtccggtgca actgcagccg acagccagag
     2221 tgctgtgagc accggctgca agccaaccag cagcgccacc gtgccggctc cgatgcccgc
     2281 cttcaccgcg gcccacacgc cgcccagata ggccgcatgc atcagcacac cggtgacgcc
     2341 caggtgcaac cactgcacgc gccccacggg ccacggagcc cgagacgcag cgatccagac
     2401 gccgaagcag acgatcgaca gcccaaagcg catcgacagg aacgtgaaag tgggcgagtt
     2461 ggacatcgcg tagcgcgcga cgatgaagcc ggtgctccag atcatcacga acaccgcagg
     2521 catcgcacgc agcgcagcgt cgccacgcgt ggtcggcagc acgctcacga agggacgcta
     2581 tctcgccttg gcgcggatct ccggcagcgc cttctggagg taatacacca tcgaccagac
     2641 ggtcagcacc gcggagatat agatcagcaa ggtgccccac caatgggtgt cgatgacacc
     2701 gaagagcatc ccgtgataca gcagaaacgg aatggcgatc atctgaacca tcgtcttcag
     2761 cttgcccagc atgtgcaccg ccacgctgcg cgaggccccg atctgcgcca tccattcacg
     2821 cagggcggaa atggcgatct cgcgaccgat gatgaccagc gcgaccagcg catgcagccg
     2881 gttcaggtgc accagcacca acagcgccgc acacacgagg aacttgtctg ccaccggatc
     2941 caggaaggcc ccgaaggacg aggtctggtt gagcctgcgc gccaggtagc cgtcggccca
     3001 gtcggtcaac gcaacgacga tgaacagcgc ggtggcgatc gtgttgcgca atacatcgtt
     3061 caggtcaaga tagaacacac cgaccaccag cggaatcgcg acgatgcgcg cccaggtcag
     3121 cagtgtcggc aggttgaaga acattcgatc gattgtgccg tgtcacggaa cggccgcgcc
     3181 catcaagagg acatcagcga tcgttcagcg tgcggggctt gaagcgacga tcgtcgttga
     3241 agaggaatgt ttcggtgaat cgccaggggc ggggaaggtg cgacacgtca ccgaaggggg
     3301 ccgcgcgcct gacggcatcg atggcaatct gcgtggtgtc cttggcctga cgcggatagc
     3361 gcaacacatc gatatgacgg atcgacccat cggcattgag atcgacggcc agcaccggaa
     3421 tcgccagcag cacgtcgggc gggcggtcgt tgtagatcag atgcgggttc gcggcggcga
     3481 tccggcgtgc cgcctgcagg tgcagttcgt tcgcgctgcg cacggtcgcc ggggcaggca
     3541 gccgcactgc tgcggcagcg cccgcagcga cggcaggcgc cgcgggtcgc tccaccggag
     3601 cgacaggcac cgcgaccggc accggcctgc ccgtgctcac cggcaccggc actggtggag
     3661 atcccggtcc ggtggcgcag cccgccagcg ccacaccgac gcaagcggcc gccctcgaca
     3721 ggcaccggac tgtctgtcga tgcaggttcc gccaatcaat gcagggcacg gtagatctcc
     3781 tcggccaggt ctttcgatat gccgtcgacc gtcagcaggt cggtcacgct cgcgctggcc
     3841 acgccgcgca cgccgccgaa ccgctgcaac agcttggccc gcttcttcgg gccgacgccc
     3901 gggatgtcct cgagcttgct gccgccggtg cgcacgctgg cgcgcttcgc gcgcatgccg
     3961 gtgatggcga atcgatgggc ctcgtcgcgg atctgcgcga tcagcatcag cgcggccgaa
     4021 tcccggccca ggtagacctt gtcccggccg tcggcgaaca ccagttcttc gagcccgacc
     4081 ttgcgcccct cgcccttttc gacgccggcg atcaggccca ggtcgaggcc cagttcctcg
     4141 aacacttcgc gcgccatgct gacctgcccc cggccgccgt ccaccagcac caggtcgggc
     4201 aatcgcagtt cgggccgggc aaacacggac tcgtcggccg gttcaccggc ctcctcgacc
     4261 tgcctggcca cctgcgctgc gagccggccg tagcgacgca tcagcacctg ccgcatcgcg
     4321 gcgtagtcat cgccgccggt gatgccctcg atgttgtaac ggcggtactg ggcgctctgc
     4381 atcttgtggt tctcgaacac gacgcaggat gcctgagtgg cctcgcctgc cgtgtgactg
     4441 atgtcgaaac actcgatgcg aaacgtgtcc agctgctcga cttccagcgc caacgcatcg
     4501 acgagcgccc gcgtgcgtgc ctgctgggat ccctcctcgg cgagcaactg cgccaggcgc
     4561 agttcggcgc ccttgatgca catgtccagc caagcccggc gtgcatcgcg cggctggtgc
     4621 tgggcggtga ccttgacacc gctctgcgcc gacagcgcgt cgatcagcgc ccggtcgacc
     4681 ggatggttca gcaccagcag cggtggcacc ccgccatcga ggtagtgctg cgcgatgaag
     4741 gcttcaagca ccagggcttc gggtgccgat gcagcttcgt ccctgcccgg gccgtcggct
     4801 gtcggcccga catccagtgc ggtcgcgtcg ccgatgtgca ccggaaagaa tggccggtca
     4861 cccaggtgcc ggccgccgcg aaccatcgcc aggttgacgc aggcgcgacc gccctgaacc
     4921 ttcacggcca ggatgtcgac atcccgcgtg gcgatgccgg tgttgtcctc gaccgattgc
     4981 tgatgcagca cccgcgacaa cgcgccgatc tggttgcgta tctcggcggc ctgctcgaac
     5041 tgaagcgctt cggcgtggac catcatctgc gcctgcagcc gatccatcac ctgctgctgt
     5101 tcaccctgaa gaaaccgctc ggcgttcgcc acgtcgcggg cgtagtcatc cgcagggatc
     5161 aatttcacgc acggccccga gcaccgcttg atctggtgca gcaggcacgg ccggctgcgg
     5221 ttgttgaaga cggtgtcctc gcaggtgcga agccggaaca ccttctggag cagctggatc
     5281 gactccttga ctgcccaggc gctcgggtaa ggaccgaagt agcgatgccg ctgatcgaca
     5341 gggccgcggt agtaggccat ccgcgggtat tgcgaagcca gcgatcccga ggcgttaccc
     5401 ggcgtcccgg tgatcttcag atacggatag ctcttgtcgt cccggaacag gatgttgtag
     5461 cgaggattga gcgccttgat caggttgttc tcgagcagca gcgcttcggc ctcggagcgc
     5521 acgaccgtcg tctccatgcg cacgatcttc gtgatcatgt ggccgatgcg ggtgccgccg
     5581 tggctcttct ggaagtagct cgaaacccgc ttcttcaggt cacgcgcctt gccgacatag
     5641 agcacgccgc cctgggcgtc gaaatagcgg tagacgccgg gcaggcccgg cagcgcagcc
     5701 acgtcggcaa gcagacgctg ccgccgctcg tcgggctcgg cggcagtcgc gccagcggaa
     5761 tcaggggtct cggacatcgc cggcgattgt gcaggccccc ggataatcga acgatgtccc
     5821 tgccctgctc acagaccccc ggaatcggac ggacctggga catcttctgc cgggtcgtcg
     5881 acaaccacgg cgacgctggc gtgtgctggc ggctggcgac cgatctcgcg tcccgccaga
     5941 tcgacgtccg gctgtggatc gatgacgccc gtgcccttgc ctggatggca ccgacgggca
     6001 ggcacggggt gcgggtgctg gcctggccgg acggcgatca ggacatctcc cacgcgctcg
     6061 atccggcgcc atcggtggtc gtggagacct tcggctgcgg actgccctca ccttacctgc
     6121 aagtcatggc tcgcgcgcgc gggcaggcgc aggcacctgt gtggatcaac ctcgaatacc
     6181 tgaccgccga gccttacgcg cagcgcagtc actgcctgcc ttcgccgcag agcagcggcc
     6241 ctgcgcgcgg catgaactca tggttcttct atcccggctt caccaccggc accgggggtc
     6301 tgctgcgcga gaccgatctg gtggccagac aggttgcgtt cgaccgcatg gcctggctgg
     6361 cgacgcacgg tatcgcgccc cgagccgacg agcgcgtggt gagcctgttc tgttacgcca
     6421 atccgaacgt gcccgacctg ctgcaagcgc tggccgcccg ccccaccctg ctgttgacga
     6481 ctcccggcca cgcgaccgag caggtcctgc aggcactgtc ggccgccgaa ctgccagccc
     6541 ggctgcgcat ccaggcgctg ccgtggttga cgcagatcga ttacgaccac ctgctctggt
     6601 cctgcgatct gaacttcgtg cggggagagg attcgctggt gcgcgcgatc tgggctggtc
     6661 gaccgttcgt ctggcagatc tacccacagg acgacagcgc acacgaagcc aagctggatg
     6721 cgttcatcgc gcaaaagctg gcaacggtgc agcccgcctg gggcgaagcg attcgccgcc
     6781 tgagctggca gtggaacagt ctcgtgggca gcacgggctc gcaggcaggc gacggcatcg
     6841 atgaatggaa tctgctgaac acgccgcagg ggatgtcggc gtggacctcg cgctgcgccg
     6901 cgtggcggga cgaattgatc gaattgccgg acctcacatc gcagttgctg agattcgtcg
     6961 ccggcccgca gtcccgcgaa gacgctaaaa tctgaggctt ttcgtttcgc gaccctggcg
     7021 gcacggaaaa cccactacac agatctcaac ggacagcgtc atgaaaatcg cacaggaaat
     7081 tcgcgccggc aacgtcatca tgcacggcaa ggaccccatg gtcgtgctga agaccgagta
     7141 cagccgcggc ggccgcaact cggccaccgt gcgcatgaag ctcaagagcc tgctgagcaa
     7201 ttcgggcacc gaagtcgtct tcaaggccga cgacaagatg gaccaggtca tcctcgacaa
     7261 gaaggagtgc acgtactcct acttcgccga cccgatgtac gcattcatgg acgccgaata
     7321 caaccagttc gaggtcgaga gcgagaacat gggcgacgcg gtccagtacc tcgaggatgc
     7381 gatgcctgtc gaggtggtgt tctacgacgg caaggccatt tcggtcgaac tgccgaccag
     7441 cctggtgcgc gagatcgtcg agacggagcc cgccgtcaag ggcgacactt ccggcaaggt
     7501 gctgaagaac gccaagctgg cgaccggttt cgagatctcg gtgccgctgt tcgtgcagac
     7561 cggcgacaag gtcgaaatcg acacccgcac ccacgagtac cgcaagcgcg tttaacgccg
     7621 gacgtctgtc gggcagacgg aaagcgggcg ccttcgggcg cccttctctt tggttgaatc
     7681 cattcttcgg catgatgctt cgatggcttt cgacttcaat gccgtctccg caccgttccg
     7741 catgcaaccc gggatgcgcc ggcttgcgcc cggcacgacc cagctgacgc caatccttcc
     7801 cggacaacag gtcttcagcg agaagctgga ggtgcttcga ttgcaccctg gccaggcctt
     7861 gctgaccagc cccgggttcg attcaggccc cgcactcgca gcgttgttcc agcaaggcgc
     7921 gacagaacat ccgcaggcgt ggcgggttgc tcccgatgga tcggttcacg ccctgcatct
     7981 gggctggtcg gtacaaggcg atcaggcgac gtcgatggac aacgatgcgc cgcaggagat
     8041 cggtgactgc ctcgcggccc tgccccccga atggcgcacg acagcgctgt tctgcctcgc
     8101 attcgccgaa gacttcgcgg tcatttgtgg cgacacgtcg atcatcccgt ggctggcggt
     8161 gtgcctgccc tcgcactggg cccccgagga aaagataggc cggcactttg ccgaggtgca
     8221 tgcgccggtg gccgacaacc acttgctgat caccgccagt gatcacctcg ccaggttggt
     8281 gaccggaacc gaccgctggc agcgccaggt ctggaccctc acccggcacc cgagcctgca
     8341 cgcacatccg gcacggctcg acgcggccga ttggcccgca gaagccgatg ccgatcaact
     8401 cgccgcacag gcctggttgc gcaccgagca tcagaccttc atcccgctcg cctcgccccg
     8461 gcaagccgtt ttcacgatac atgtcgaggt gcgcccactc gccctggcaa tcaccgaacc
     8521 cgcacaggcc cggcgcctgc atgacgcgct ggagaccatg accgcctcgg tactggccta
     8581 ccgcagcctg acgaccgctc gcgatcgcct gctctcgtgg ctgcatgcgc ggagcatgga
     8641 gggcgcaggg tgagcgaccc cgtcgcggcc ggccagtgca aagacgccac agcctgcgct
     8701 gcggtgttga ccgagctttg gaaggacgac gatcgtttca tcgtgctcga cgatcgcttc
     8761 gatggaggcg agcgcttcca aagcctgctc caggcctggc gtgacgaccc ctgtcggtgt
     8821 gcgcagctcc atgtgatcac cgcgtccccc gaaatcgtcg acggctgcac ttcgtccgca
     8881 ctgcgcgagg agatcgccga tcgctggccg cccatcactg caaacatgca ccgtctcgcc
     8941 ttcgacggcg gacgcgtcca gtggatgctg gtgcctacgc cgatgcaggc tgcattgcgc
     9001 accctggtcg ccgcagtcga cgtgttcgcc tgctcggccc cgcctgctgc ggacccctac
     9061 gcaacgcgca gatgggcgaa ggcgctggca aggctggccg gcgctgacgc ccggttgctc
     9121 ttcgatgcgg gatcggtgtg cgactcgatg cgacacgcct tgcgctcggc gggcttcgcc
     9181 gagcagcccg acaccgaccc cgcggatcgg cgaggcgctg acatcgagcg ctggcgtcac
     9241 gctccgcagt tcacgtcgag gcagccgcct gcgcgcagcg gcgctggcgc gcgctcaagg
     9301 gatccggtgc tgatcgtcgg cgccggactg gccggatgcg ccatggcctg ggcccttgcg
     9361 gagcagggcc gcgaaagctt cgtgttcgag cggcgcgcaa cggtagcgct cgaaacctcc
     9421 ggcaaccccg caggcctgtt ccacggcatc gtcaatccgc aggacggcgc ccatgcgcgc
     9481 ttcaaccgtg gcgccgcgct ggaagctcag cgcgccgtgg ccatcgcact ggcgcagcac
     9541 caggtgcaag gcagcgcgcg cggactgctg cgactcggaa atgccctggg caatgacgat
     9601 ctgccgacga tgcgagccac gctcgcacgg ctgggtctgc cgggggacta cgtgctggct
     9661 gtctcggcac aacaggcctc ggaactcagc ggattggcgt tgcgtcaggc ggcctggtgg
     9721 tacccggggg gcggctgggt gcagcccgct ggcctggcgc gatcattcct ggagcgggcg
     9781 ggcccccggg ttgcgctgag agccgacagt tccgtgcact cactgacacg ggagtccggc
     9841 ctgtggcagc tgcgcgatga acgcggctcg atcctcgcgg cagccgatgt cgtcgtgctg
     9901 gccaatgccg gggacgcact gcgcctgctg cagccctgga tgcccgcgtc gcagccgcac
     9961 ttctggccgc tgttgagggt gcgcgggcag atcaccacgc tgcccgttgc ggcgatcggc
    10021 gcactgggtc tgcccagggt cccgatcgcg ggcgcgggtt atctgctgcc gggggtcgac
    10081 gggctcgcca tcttcggcgc cacatcgcag gcggacgatg acgatccggc agtgcgcgac
    10141 agcgaccacc ggctgaatct ggatcagttg tcacggctga ccggcatcga ccaccgcatc
    10201 gaaccgactg ctcacggcat cgatggtcgc accggctggc gatggagcac ggacgatcga
    10261 ctgccggtgc tcggtgccgt gccggactgg acagctgcac tgggagcaag gcgactggaa
    10321 cagccggtcc atgtgccgcg cctgcaaggg ctgtacgcct gcacggcttt cggatcaaga
    10381 ggcatcacct gggcggccct ggcggcgcag gtggtggctg catcgatcac cggggctccg
    10441 atgccgatcg aggcaggcct gctggacagc gtcgacgctg cccgctgggt ggtccgtgca
    10501 gcaacccgcc gcacaaggcg ggtcgaatga atgacggacc ggtggacgcg tcaggcggag
    10561 ggtgagcctg agtccgcacc ggcaccgggc gatgctccag ccgcttcgtc ggatgccgac
    10621 tcggtaccgc cctcaccgcc ctgcttgcgc tcggacttct ccttcacttt ctcttccttc
    10681 ttcttcttct tggccagctc gcgctgacgc ttttcgaacg aatagttggg cttggccatg
    10741 ggcatgcatc cacgaaattg attcaggcac cgagattacg ctgttttcag cgcattccgg
    10801 ccgattcaag cctcatctgc gcggggcccc gtagtccacc gtctcgacac catccggcgt
    10861 gccgaaaagg cacaggctgg ccctgtgacg agcgaagatg cccaccgtca ccacccccgg
    10921 ccactggttg atctcctgtt ccatcgccag cgggtcggca atcgccaggc catgcacgtc
    10981 gagcaactgg caaccgttgt ccgtcaccac gcccgcacga agcacggctc ggccgccaaa
    11041 cgcagtgaag cgccgcgcga tctgcgacgc cgccatcggg atcacctcga ccggcagcgg
    11101 gaagcgcccg agcaccggca cgcgcttgga gacatctgca atgcagacga agcgatcagc
    11161 caggtcggcg acgatcttct cgcgggtcag cgccgcgccg ccgcccttga tcatgtggcc
    11221 gttcggatcg atctcgtcgg cgccgtcgat gtagacggga atgcgatcga tcgcattgct
    11281 gtcgagcacc gcgatgccat gggcacgcag ccgctcgctg ctcttctcgc tgctggacac
    11341 cgcaccgacg atccgctcac ggatgccggc cagcgcgtcg atgaagcagt tgaccgtgga
    11401 gccggtgccc acccccacca cgctgcccgg tgccacatgg gccagcgcag cctgacccac
    11461 catcgccttc aattcgtctt gagtcatggg gaaaaccgtt tgagacaatc gagaattatc
    11521 ccgcgctgaa ttcatggcc
//
LOCUS       DAOORV010000112        11363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7127_length_11363_cov_7.039795, whole genome shotgun sequence.
ACCESSION   DAOORV010000112 DAOORV010000000
VERSION     DAOORV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_11363_cov_7.039795"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKV56_13520"
     CDS             complement(<1..1477)
                     /locus_tag="PKV56_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOX68877.1"
                     /translation="MKCRTGGLCSRALLAAAWLLGAASAQAGELSAAYVFPLPRSAAD
                     IRADCQDMLADLKQSESRLAQAADSSAPSLLPELDAMTRRAEDTLGPLAVLVAVSPRK
                     AIRDAGEACDRDYQTYASGFLQNAAVYARLNQVQPADDIDRRFLRDTLDSFEDAGVAL
                     PADRQQRVREINDELTALTQAFERRIREDRTQLFFTKAELAGVPAGVWKRVPRDAKGR
                     YRLGLDQATVFPVLENATVSATRERMWRAFQSMGGQQNIETLAALTQRRRELARLLGH
                     ASYGDFVLRRRMARGEAEVQAFLGTVKDAVRSRELTDLAELRIAKAVHLKQGVDRAAL
                     ERWDTSFYAERVRRARFDVDQKQFRRHFPPDASLRFVFRLAERLFSVTFAPLEGPNRQ
                     RLWHTEARAYVVTDSETKAPLGTLFVDLYPRADKFNHAAVWGFRNASSLADRRPAAAL
                     VVNFNRQGLTLDELETLLHEFGHALHSLLSKTRYAAQGGTSV"
     gene            complement(1506..1787)
                     /locus_tag="PKV56_13525"
     CDS             complement(1506..1787)
                     /locus_tag="PKV56_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOX68878.1"
                     /translation="MIRHIVMWSLHNPADAPRFKALLDSCASLVPGIVEFDVGIRETG
                     LDANIDVVLVSSFTDSAQLDAYQSHPHHKEVGAQLGTLRASRHVLDYAR"
     gene            complement(1792..1977)
                     /locus_tag="PKV56_13530"
     CDS             complement(1792..1977)
                     /locus_tag="PKV56_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68879.1"
                     /translation="MNASTLEKITWPLVYGGILVLLLGISTLSLEAAVGWPIVIGGAF
                     LTLAGVLAIYLRSRIKD"
     gene            2131..3648
                     /locus_tag="PKV56_13535"
     CDS             2131..3648
                     /locus_tag="PKV56_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOX68880.1"
                     /translation="MNSKDNTPVADGLPTLAHGPEWTWRNSFRALGCEYHTELEPQPM
                     RESHWVAHSDPCANLLGLPADWRSDPRWNALEVFSGNALWPGMQPLASVYSGHQFGVW
                     AGQLGDGRALWLGEIDTQSGPMELQLKGSGPTPFSRMGDGRAVLRSSIREFLCSEAMH
                     GLGIPTTRALCITGSSLPVRRETIETAAVVTRVAPSFIRFGHFEHFAQHDEPSALKQL
                     ADFVIEHHYPECRNTPQPYVAMLGIAARRTAELLAQWQAVGFCHGVMNTDNMSILGLT
                     LDYGPFGFLDSYDPGHICNHTDHKGRYAFSRQPSVAFWNLHALAQALMPLIGNRDEAL
                     DAIEIYKPFYAEAMRRQLRTKFGFTTTEAGDAGLIDWLFKQMAYEKTDHTILFRRLSR
                     FSTAEGVDNSEVMGLFKDRKFSEIWAKAYGDRLRSESSDDAVRSEAMNRVNPSIVLRN
                     HLAEAAIRQAQAGDFSEVQRLVTALQNPFDTSHDSSTDAGFPPDWAQGIEVSCSS"
     gene            3672..4082
                     /gene="msrB"
                     /locus_tag="PKV56_13540"
     CDS             3672..4082
                     /gene="msrB"
                     /locus_tag="PKV56_13540"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOX68881.1"
                     /translation="MKDDKPREVQKTDAEWRAQLDATQYEVARQAGTERAFSGKYWDH
                     WEPGRYHCIGCDTPLFEASTKFDAGCGWPSYCEPINSTVIERITDRSHGMVRIEVRCN
                     RCGSHLGHVFNDGPAPTGERFCINSASIDFKAPV"
     gene            4138..4782
                     /locus_tag="PKV56_13545"
     CDS             4138..4782
                     /locus_tag="PKV56_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HOX68882.1"
                     /translation="MKLLLDFLPIILFFGTYKYAESHKDWAAAFATEHFGFIVSGGIV
                     GAKEAPVLLSTVVVIVATFAQIAYLYARGRKIDTMLWVSLGLVVVLGGATIWFHNEEF
                     IKWKPTVLYWAMALALWSGPALFGRNLLQKMMGDQVRLPAPVWSRLNVAWIVFFFSMG
                     VLNRYVAWRYSTDTWVNFKLFGSMGLMILFMVAQGFYLSRHLEPDEAESNLPPR"
     gene            4821..5087
                     /locus_tag="PKV56_13550"
     CDS             4821..5087
                     /locus_tag="PKV56_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOX68883.1"
                     /translation="MSVSASDIESTLRAALSPSELTVRDDSHLHAGHAGAREGRHYSV
                     RLVSECFAGLGRVARHRLVYDSLKPQMQAGIHALVIDARAPGEA"
     gene            5215..6024
                     /locus_tag="PKV56_13555"
     CDS             5215..6024
                     /locus_tag="PKV56_13555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX68884.1"
                     /translation="MNKPLLTSTALASTLVIALLAPLPALAQNLALVNGKAVPKARVE
                     ALMQQVTKQGQPRTPELEAQVRDEVALREMFVQEAEKRGLAANAEYKTQMEFARQTIL
                     IRELFTQFQSANPVTDAEIQAEYEQVQKQSAGTEYRARHILVETEDQAKALIAQIKGG
                     AKFEELAQKNSKDTGSGANGGDLDFANPVSFVPEFSQAMVKLKKGEMTETPVKSQFGF
                     HIIRLDDTRESQLPPLAEVKQQIQQKLGQGKIAKFRDDIRAKYKTDYKFAN"
     gene            complement(6053..6739)
                     /locus_tag="PKV56_13560"
     CDS             complement(6053..6739)
                     /locus_tag="PKV56_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX68885.1"
                     /translation="MSDPIIAVDRITKCVSDPGGSLTILHEISFTLQRQQSVAIVGAS
                     GSGKSTLLSIIAGLDTPSSGSVRLAGTDLFSLDEDARAAVRAAHVGFVFQSFQLLGNL
                     TALENVMLPLELQGLGDARSRATEMLARVGLGDRLRHYPKVLSGGEQQRVALARAFVV
                     KPDVLLADEPTGSLDFATGERVMALMFELNRETGTTLVLVTHDRGVAARCDRQLRIEA
                     GRVEPETAPV"
     gene            6768..7451
                     /locus_tag="PKV56_13565"
     CDS             6768..7451
                     /locus_tag="PKV56_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HOX68886.1"
                     /translation="MDPARRRWFQTLFHCTALMCLASAASAAQSQTPVAALAHAAQAG
                     KPPRHTVLVVGDSLSAEYGLKRGSGWVALLEQRLFKEQVSARVINASISGDTTSGGLS
                     RLPALLKQHRPTLVVLELGGNDGLRGLPLVDAQRNLAQMAQAAKSAGARVLVVGVQIP
                     PNYGRRYNDELAEMFVSVARTEGAALVPFLMKDVPDEQYQADRIHPMEVGHPTMLANV
                     WPALKPLLR"
     gene            complement(7492..9420)
                     /locus_tag="PKV56_13570"
     CDS             complement(7492..9420)
                     /locus_tag="PKV56_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68887.1"
                     /translation="MTCVNKSSHRAVRGSVALLMCGVLLQAPACAAPPYDAGPVGSQD
                     GYTVSTQDVDPPGRVGRLSEVLGQVWIYSPEDREWISAERNRPLTTGDRLSTDALGRA
                     EMRVGSTVVRLDSSSELEVLRLDDDRLRLQLHRGSSMVRLRDSELAAQFEMVTAEGRF
                     KADVAGGYRFDRAPNNTQATVISGQLNYEGRSQALTITAGQRAEFLLDSNARAQYAIV
                     EPKRDAFALWNADRDRYERDELASERYVSPEMTGIEDLDRHGRWDQDPEYGAVWIPRG
                     VAAGWAPYTTGRWTWVPPWGWTWVDVAPWGFAPFHYGRWVHRGSIWAWAPGSYVARPI
                     YAPALVGWAGGRPPVRRPGVSIDISIGRGGGWFPLSPRERYVPGFRASPRYERELNGS
                     HGSYFNAPRDRGDRNDRGDRNDRNDRNDRNDRNDRNDRGDRGDRNDRNDRGDRGDRGD
                     RPPAPIPQASPGRGVPDHVTAVPPQVTSTRPGVAPMLPTRPREDDRGRAPWPRDEPPN
                     LAVPGGGNPGAPSGLPLPRPAARPAPTPQLLPTPAARPAPAAPDNAELMRNPQTRNQW
                     FETVRENHEKRQAPAVREQPAAPVQPSTERDSGRNPQRGAEAGDVRRDRPGLPPASDP
                     RSDPRDGRDPRGDGRNAN"
     gene            complement(9547..10083)
                     /gene="def"
                     /locus_tag="PKV56_13575"
     CDS             complement(9547..10083)
                     /gene="def"
                     /locus_tag="PKV56_13575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOX68888.1"
                     /translation="MTVREILKMGDPRLLRIAKPVSAFGTRELDALIADMFDTMHAAE
                     GAGLAAPQIGVDLALVIFGFRQNPRYPDADAVPETVLINPAITPLDAVEEDGWEGCLS
                     VPGLRGVVPRWARIRYTGFDQHGHSIDREADGFHARVVQHECDHLIGTLYPMRVRDFR
                     QFGYTSVLFPDLDAGADD"
     gene            complement(10195..>11363)
                     /gene="ligA"
                     /locus_tag="PKV56_13580"
     CDS             complement(10195..>11363)
                     /gene="ligA"
                     /locus_tag="PKV56_13580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOX68889.1"
                     /translation="REPRWAVAHKYPAQEQMTKLLGIEIQVGRTGKLTPVARLEPVFV
                     GGTTVSNATLHNEDEVRRKDVRAGDTVIVRRAGDVIPQVVGVVHDTTGSADAALRGEP
                     FDLYQRLGGACPVCGSPIGREEGEVDWRCTGGLTCPAQRKEAILHFAGRRAMDIEGLG
                     DKLVDQLVDAGLIRTLPELYTLDLAKLRALDRMGDKSAANLLAGLEASKRTTLARFLF
                     ALGIRHIGETTAKDLARHFGSLERIVCATETQLLEVNDVGPVVAHSVRSFFDQPHHRE
                     IVEQLRAVGLTWQENEGVASADAAPAGVEGVSGKTFVLTGTLPTLARDAAKDLIEAAG
                     GKVSGSVSKKTHYVVAGSEAGSKLEKANELGVTVLDEAALLALLGPTGKVELKA"
BASE COUNT         1951 a         3905 c         3683 g         1824 t
ORIGIN      
        1 gcacgctggt gccgccctgc gcggcgtagc gcgtcttcga gagcagcgaa tgcagagcgt
       61 gcccgaattc gtggagcagc gtctccagct cgtcgagggt caggccctgc cggttgaaat
      121 tgaccaccag cgccgcagcc ggccgccgat cggccaggga cgatgcattt cggaatcccc
      181 acaccgccgc atggttgaac ttgtcggcgc gcgggtacag gtcgacgaac agcgtgccga
      241 gtggcgcctt cgtctcgctg tcggtgacga cataggcacg cgcctcggtg tgccagaggc
      301 gttgcctgtt cggcccttcc aagggagcga aggtcacgct gaacaatcgc tccgccagcc
      361 ggaagacgaa tcgcaggctc gcatcgggcg gaaagtggcg gcggaactgt ttctgatcga
      421 catcgaagcg ggcacgacgc acccgctcgg catagaacga ggtgtcccag cgctcaagcg
      481 cagcccggtc cacaccctgc ttcaggtgca cagccttcgc gatgcgcagc tccgcgaggt
      541 cggtcagctc gcgcgatcga accgcgtcct tcaccgtgcc caggaaggcc tgcacctcgg
      601 cctcgccacg agccatccgg cgcctcagca cgaagtcgcc atacgatgcg tggccgagca
      661 accgcgcgag ttcgcgacgg cgctgcgtga gcgcagccag tgtctcgatg ttctgctgcc
      721 cgcccatcga ctggaaggcg cgccacatgc gttcgcgggt ggcggacacg gtggcgttct
      781 cgagcaccgg aaacaccgtg gcctgatcca ggcccagacg gtaccgaccc ttcgcatcgc
      841 ggggcacgcg cttccagacg ccggccggca cgcctgccag ttcggccttc gtgaagaaga
      901 gctgcgtgcg gtcctcgcgg atgcgacgtt cgaaggcctg cgtcagcgcg gtcagttcgt
      961 cattgatctc gcgcacgcgc tgctgtcgat cggccggcag cgccacaccg gcgtcctcga
     1021 acgagtcgag cgtgtcgcgc aggaatcggc ggtcgatgtc atcggccggc tggacctggt
     1081 tcagccgtgc atagaccgcg gcgttctgca ggaagccgga cgcgtacgtc tgatagtcgc
     1141 ggtcgcaggc ttcgccggca tcgcgtatcg ccttgcgcgg gctcaccgcg accagcaccg
     1201 ccagcggccc gagcgtgtcc tcggcccgcc gcgtcatcgc atccagctcg ggcagcagcg
     1261 agggcgcgga cgaatcggcg gcctgcgcga ggcgggactc ggactgcttc aggtcggcca
     1321 gcatgtcctg acagtcggcg cgaatgtcgg ccgcgctgcg cggcaagggg aacacgtagg
     1381 ccgcggacag ctcgcccgcc tgcgcgctcg cagcacccaa cagccaggca gcggccagca
     1441 gcgctcggct acagaggccg cctgtgcggc atttcacggc gcgagcaggc agcgtgtcgg
     1501 ctcggtcagc gtgcgtaatc gagcacatgc cgactcgcgc gcagcgtgcc gagctgtgcg
     1561 ccgacttcct tgtgatgcgg gtggctctgg tacgcatcga gctgggccga atcggtgaag
     1621 ctcgacacca gcaccacgtc gatgttcgcg tcgagcccgg tctcgcgtat gcccacgtcg
     1681 aactcgacga tgcccggcac gaggctcgcg cacgagtcga gcagcgcctt gaagcgcggc
     1741 gcgtcggccg ggttgtgcag ggaccacatg acgatgtgac gaatcatttg ctcaatcctt
     1801 gatgcgcgaa cgcagataga tggccagcac gcccgccaac gtcaggaatg cgccgccgat
     1861 gacgatcggc cagcccaccg ccgcctcgag cgagagggtc gagatcccca gcagaagcac
     1921 gaggatgccg ccatagacca gtggccaagt gattttctcg agggtggaag cattcatggg
     1981 tgatggtggc aggcgcacag gcagcgcccg gcagtgtggc accaagtgtc gacgcaggcc
     2041 attccggcga cgggtcgagc ccctgggccg cgcgacacgc acttatccct accgcagccc
     2101 gcatccggac gcgtgaatac actggccgcc atgaactcaa aagacaacac cccggtggcg
     2161 gacgggctgc ccacgctggc acatggcccc gaatggacct ggcgcaacag ctttcgagcc
     2221 ctggggtgcg agtaccacac cgaactggag ccgcaaccaa tgcgcgaatc gcattgggtt
     2281 gctcacagcg acccgtgcgc gaacttgctg ggcctgcccg cagattggcg gtccgacccg
     2341 cgatggaacg ccctggaagt gttcagcggc aatgcactgt ggcctggcat gcagcccttg
     2401 gccagcgtct acagcggcca ccagttcggc gtgtgggccg gacaactcgg cgacggccgc
     2461 gcgctgtggc tgggcgagat cgacacgcaa agtggcccga tggagctgca gctcaaggga
     2521 tccggaccga cgcctttctc gcgcatgggc gacggccggg cggtgttgcg ctcctcgatc
     2581 cgcgagttcc tttgctccga agcgatgcac ggactgggca ttccgacgac gcgcgcgctg
     2641 tgcatcaccg ggtcgagcct gccggtgcga cgcgagacga tcgagaccgc cgccgtggtg
     2701 acccgcgtgg caccgagctt catccgcttc ggccatttcg agcatttcgc gcagcacgac
     2761 gagccctctg cgctgaagca actggccgat ttcgtgatcg agcaccacta ccccgagtgc
     2821 cgcaacaccc cgcagcccta cgtcgcgatg ctcggcatcg cggcacgacg cacggcggag
     2881 ctgctggcgc agtggcaggc ggtgggcttc tgccacggcg tgatgaacac cgacaacatg
     2941 tccatcctgg gactcacgct cgattacggc ccgttcggct tcctcgacag ttacgacccg
     3001 gggcacatct gcaaccacac cgatcacaag ggacgctacg cgttctcgcg ccaaccgagc
     3061 gtggcattct ggaacctgca tgcgctcgca caggcgttga tgccgctgat cggcaaccgc
     3121 gatgaggcac tcgatgcgat cgagatctac aagccgttct acgccgaggc gatgcgtcgc
     3181 cagttgcgca ccaagttcgg attcaccacc accgaggccg gtgacgccgg gctgatcgac
     3241 tggctgttca agcagatggc ctacgagaag accgatcaca cgatcctgtt ccgacggctg
     3301 tcacgcttca gcaccgccga gggcgtcgac aacagcgagg tgatgggact gttcaaggat
     3361 cgcaagttca gcgagatctg ggcgaaggcc tatggcgatc gcctgaggtc cgagtccagc
     3421 gacgatgccg tgcgttcaga ggccatgaac cgcgtcaacc cgagcatcgt gctgcgcaac
     3481 cacctggccg aggctgcgat acgccaggcc caggcgggcg acttctccga ggtgcagcgc
     3541 ctggtgacgg cgctgcagaa cccgttcgac accagccacg attcgtcgac cgatgccggc
     3601 tttccgcccg actgggcgca gggcatcgaa gtctcctgtt cttcctgaaa ccggcacgcg
     3661 agcaagcaac catgaaggac gacaagcccc gcgaggtgca gaagaccgac gccgaatggc
     3721 gagcccagct cgatgcaacg cagtacgagg tcgcccgcca ggccggcacc gagcgcgcgt
     3781 tctcgggcaa gtactgggac cactgggagc ccggccggta ccactgcatc ggatgcgaca
     3841 cacctctgtt cgaggcgagc acgaagttcg atgccggatg cggctggccc agctactgcg
     3901 agccgatcaa cagcacggtg atcgaacgca tcaccgatcg cagccatggc atggtgcgca
     3961 tcgaggtgcg ctgcaatcgc tgcggctcgc acctcggtca cgtgttcaac gatggccccg
     4021 cgccaaccgg tgagcgcttc tgcatcaact cggcttcgat agacttcaag gcccctgtct
     4081 gacagcgcgg cccatccatc tcgcggatgg ccctgaaacg ctgattccca cgcatccatg
     4141 aagctgctgc tcgacttcct gccgatcatc ctgttcttcg gcacctacaa gtacgccgaa
     4201 tcccacaagg attgggctgc cgcattcgcc accgagcatt tcggcttcat cgtgtcgggc
     4261 ggcatcgtcg gtgcgaagga ggcaccggtg ctgctgtcga ccgtggtggt gatcgtcgcg
     4321 accttcgcgc agatcgccta cctgtacgcg cgtggccgca agatcgacac gatgctgtgg
     4381 gtcagcctgg ggctcgtggt ggtgctgggt ggtgccacca tctggttcca caacgaagaa
     4441 ttcatcaaat ggaagcccac ggtgctctac tgggcgatgg ccctggcact gtggtccggc
     4501 cccgcgttgt tcggccggaa cctgctgcag aagatgatgg gcgatcaggt gcggctgccc
     4561 gcccccgtct ggtcacgtct caatgtggcc tggatcgtgt tcttcttctc catgggcgtg
     4621 ctgaaccgct atgtggcttg gcgctattcc accgacacct gggtcaactt caagctgttc
     4681 ggcagcatgg gcctgatgat cctgttcatg gtcgcccagg gcttctacct gagccgccac
     4741 ctcgaacccg acgaggcgga atcgaacctt ccaccccgct gagcgcgtac accgccccac
     4801 cagcagcaca ggccttgagc atgagtgttt ccgcttccga catcgaatcc actctgcgcg
     4861 ccgcgctgag cccctccgaa ctgacggtgc gcgacgacag ccacctgcac gcgggacatg
     4921 caggcgcccg cgaaggtcgg cactacagcg tccgcctggt cagcgagtgc ttcgccgggc
     4981 tgggccgcgt ggccagacac cggctcgttt atgattcatt gaagccgcag atgcaggctg
     5041 gaatccacgc gctggtgatc gacgcgcgtg cccccggcga agcctgaccg cagcgccatc
     5101 agcgaactcc tcccggtccc aaccagccac tctggtcctg cggatcgccg agcggcaaat
     5161 tttttgctgc tcctcacccg cccctttacc attcgagttg aaagcctcac tcccatgaac
     5221 aagccgctcc tcacctccac cgcactggcc tccacgctgg tcatcgcctt gctggcaccg
     5281 cttcccgcgc tggcacagaa cctggccctc gtgaacggca aggccgtgcc gaaggcgcgc
     5341 gtcgaagcgc tgatgcagca ggtcacgaag caaggccagc cgcgcacgcc cgaactcgag
     5401 gcacaggtgc gcgacgaggt cgccctgcgc gagatgttcg tccaggaagc cgagaagcgc
     5461 ggactcgccg ccaacgccga atacaagacg cagatggaat tcgcccgtca gaccatcctg
     5521 atccgcgagt tgttcacgca gttccagagc gccaacccgg tgaccgatgc ggaaatccag
     5581 gccgagtacg agcaggtcca gaagcagtcc gctggcaccg agtaccgcgc ccgtcacatc
     5641 ctggtcgaaa ccgaagacca ggcgaaggcg ctgattgcgc agatcaaggg cggggccaag
     5701 ttcgaggaac tggcgcagaa gaactcgaag gacaccggct cgggcgccaa cggcggcgac
     5761 ctcgatttcg cgaaccccgt gtccttcgtg cccgagttct cccaggcgat ggtcaagctg
     5821 aagaagggcg aaatgaccga gacaccggtc aagtctcagt tcggcttcca catcattcgc
     5881 ctcgacgaca cgcgcgagtc gcagctgccg ccgctggccg aggtcaagca gcagatccag
     5941 cagaagctgg gtcagggcaa gatcgcaaaa ttccgcgacg acatccgcgc aaagtacaag
     6001 acggactaca agttcgcgaa ctgatcggcc tggagctgag gccggccctc gctcacacgg
     6061 gggcggtctc tggttcaacc cgcccggcct cgatgcgcag ctgtcgatcg cagcgcgccg
     6121 ccacgccgcg atcgtgcgtg accagcacca gcgtcgtgcc ggtttcccgg ttcagttcga
     6181 acatgagcgc catcacgcgc tcgccggtgg cgaaatcaag gctgcctgtc ggttcgtcgg
     6241 cgagcagcac gtcgggtttc acgacaaacg cgcgcgccag agcgacccgc tgctgttcac
     6301 cgcccgacag caccttggga tagtgcctca ggcgatcgcc cagcccgacc cgcgccagca
     6361 tctccgtggc gcgcgagcgc gcatcgccga gcccctgcag ttcgagcgga agcatcacgt
     6421 tctcgagcgc ggtcaggttg cccagcagct ggaaactctg gaacacgaaa ccgacgtgag
     6481 cggcccgcac cgcggcgcgc gcgtcctcgt cgaggctgaa gaggtcggtg cccgccagcc
     6541 gcaccgatcc ggaggacggg gtatccaggc cggcgatgat ggacagcaag gtgctcttgc
     6601 ccgatcccga ggcgccgacg atggccaccg actgttgtcg ttgcaaagtg aaactgatct
     6661 cgtgcaggat ggtcaatgaa ccacccgggt cggacacgca cttcgtgatc cggtctacgg
     6721 caatgatggg atcggacatg aatacttcgc taagacgatc gacaacgatg gaccccgccc
     6781 gccggcgttg gtttcaaacc ctgtttcact gtaccgcgct gatgtgtctg gccagcgcag
     6841 cttcggccgc gcaatcgcag actccggtgg ccgcgctggc ccacgcagcc caggctggaa
     6901 agccgcctcg gcacacggtg ctggtggtcg gggacagcct gtcggccgag tacgggctca
     6961 agcgcggcag cggatgggtc gcactgctcg agcaacgact cttcaaggaa caggtttcgg
     7021 cacgcgtgat caacgccagc atcagcggcg acaccacgtc gggcggcctt tcgcgcctgc
     7081 cggccctgtt gaagcagcac cggccgacac tcgtggtcct cgaactgggt ggcaacgacg
     7141 ggctgcgcgg attgccgctg gtggacgccc agcgcaacct cgcccagatg gcacaggccg
     7201 caaagtcagc cggggcacgg gtgctggtgg tcggcgtgca gatcccgccg aactacgggc
     7261 gccgctacaa cgacgagctg gccgagatgt tcgtctcggt ggccagaacc gaaggcgcgg
     7321 cgctggtgcc cttcctgatg aaggacgtgc ccgacgagca ataccaggcc gaccgcatcc
     7381 atccgatgga ggtgggacat ccgacgatgc tggccaacgt gtggccggca ctgaagcccc
     7441 tgctgcgctg acgccggcgg gcacccgcgc tgcgggccgc gatgggcggc ttcagttcgc
     7501 gttgcgcccg tcgccccgcg gatcgcgccc atcgcgcgga tccgaccgcg ggtcactggc
     7561 cggaggcagt cccggccggt cgcgacgcac gtcgcctgcc tcggcgccgc gctgcggatt
     7621 gcggccgctg tcccgctcgg tcgacggctg gacaggcgca gcaggctgtt cccgcaccgc
     7681 aggcgcctga cgcttctcgt gattctcgcg aaccgtttcg aaccactgat tgcgtgtctg
     7741 cgggttgcgc atcagttcgg cgttgtccgg cgcagcgggt gccggccgcg ccgccggtgt
     7801 cggcaggagc tgcggggtcg gggcgggacg agcagccggc cgaggcaatg gcaggcctga
     7861 gggtgcgccc ggattccccc ctcccgggac ggccaggttc ggtggttcat cccgcggcca
     7921 gggcgcgcgg ccacggtcgt cctcgcgcgg acgcgtcggc aacatcggag ccacgccggg
     7981 ccgggtgctg gtcacctggg gaggcaccgc ggtgacgtga tccggcacac cgcgcccggg
     8041 ggaagcctgc ggtatcggcg ccggtgggcg atctccacga tctccacgat ctccacggtc
     8101 gttgcgatcg ttgcggtcgc cgcggtcgcc gcggtcgttg cggtcgttgc ggtcgttgcg
     8161 gtcgttgcgg tcgttgcggt cgttgcggtc gcctcgatca ttgcggtcac cccgatcgcg
     8221 cggtgcattg aagtaggagc cgtgcgaacc gttcagttcc cgctcgtagc gcgggctggc
     8281 acggaagccc gggacatagc gctcgcgcgg cgaaagcggg aaccagcccc cgccgcgccc
     8341 gatcgagatg tcgatactga cgcccggccg gcgcaccggc ggcctgccgc cggcccatcc
     8401 caccagcgcc ggggcgtaga tcggccgcgc gacataactg ccgggcgccc aggcccagat
     8461 gctgccgcgg tggacccatc gaccgtagtg gaatggtgca aaaccccacg gcgccacatc
     8521 cacccaggtc cagccccagg gcggcaccca ggtccagcgg ccggtggtgt acggggccca
     8581 gccggcggcg acaccacgag ggatccagac cgcgccgtac tccgggtcct gatcccagcg
     8641 accatgtcga tccaggtcct cgatgccggt catctcggga gagacgtagc gttccgacgc
     8701 cagttcatcg cgttcgtagc ggtcgcggtc ggcattccac agcgcaaagg catcgcgctt
     8761 gggctcgacg atcgcgtatt gcgcccgtgc attcgaatcg agcaggaact cggcccgctg
     8821 cccggcggtg atcgtcagcg cctggctgcg gccttcgtag ttcaactgcc cgctgatcac
     8881 cgtggcctgc gtgttgttcg gcgcccggtc gaagcggtag ccaccagcga cgtcggcttt
     8941 gaaccggcct tcggcggtca ccatctcgaa ctgcgcagcg agttcgctgt cgcgcaggcg
     9001 aaccatgctc gaaccccggt gcaactgcag tcgcagtcgg tcatcgtcga ggcgcagcac
     9061 ctcgagctcg ctgctcgagt cgagccgcac caccgtcgat cccacgcgca tttcggcgcg
     9121 acccagggcg tcggtcgaga gccggtcgcc ggtggtcaac ggccggttgc gctcggcact
     9181 gatccattcg cgatcctcgg ggctgtagat ccagacctgg ccgagcacct cggacaggcg
     9241 gcccacgcga ccgggcgggt cgacatcctg agtcgacacg gtgtagccat cctggctgcc
     9301 gaccggaccg gcgtcgtacg gtggcgcggc gcaggccggc gcctgcagca gcacgccgca
     9361 catcagcagc gcaacgctgc cgcgcaccgc gcggtgtgaa gacttattga cgcatgtcat
     9421 ggggttcccc ttcatgtcac tccacaacgg atgggctggc gggcgagacg acagcctcac
     9481 gccgcagcaa cacagcttta cacccgcgac acgcgcgccg tcgataccgg ccagtggccg
     9541 ggcccctcag tcgtccgcgc cagcgtcgag gtcggggaac agcacgctgg tgtagccgaa
     9601 ctgcctgaag tcgcgtacac gcatgggata aagcgtgccg atcaggtgat cgcactcgtg
     9661 ctgcacgacg cgtgcatgga agccgtcggc ttcgcggtcg atggaatggc catgctgatc
     9721 gaagccggtg tagcggattc gcgcccagcg cggcaccacg ccgcgcaggc cgggaaccga
     9781 cagacagcct tcccagccgt cttcctcgac ggcatccagc ggcgtgatgg ccggattgat
     9841 cagcaccgtc tcgggtaccg catcggcatc gggatagcgc gggttctgcc tgaaaccgaa
     9901 gatcaccagc gccagatcga caccgatctg cggcgccgcg agcccggcac cttcggccgc
     9961 atgcatggtg tcgaacatgt cggcaatcag cgcatcgagc tcacgagtcc cgaatgcgga
    10021 caccggcttc gcgatgcgca gcaacctcgg atcgcccatc ttcagaattt cacgaacggt
    10081 catcgcagcc tttcaagcct ccagcagaac atcgagtgcg cacgtcacgg cgcctcgcag
    10141 cagggactgt agttcgcgcc gtcgttctcg cgccagacgc acgctaccga aggatcaggc
    10201 tttcaactcg acctttcccg tcgggcccag caacgcaagc agcgctgctt catcgagcac
    10261 cgtcacgccg agttcgttcg ccttctcgag tttgctgccc gcctcgctgc cggcgacgac
    10321 gtagtgggtc ttcttcgaca ccgagccgct caccttgccg ccggccgctt cgatcagatc
    10381 cttggcggca tcgcgggcca gcgtcggcaa ggtgccggtt agcacgaaag tcttgccgga
    10441 cacgccttcg acccctgctg gcgctgcatc agccgaggcc acgccttcgt tttcttgcca
    10501 cgtgaggccg accgcgcgca gctgctcgac gatttcgcgg tggtgcggct gatcgaagaa
    10561 gctgcgaacg ctgtgcgcga ccaccggtcc gacgtcgttg acttcgagca gctgagtctc
    10621 ggtcgcgcag acgatccgct ccagcgaacc gaagtgacgg gccaggtcct tcgcggtcgt
    10681 ctcgccgatg tggcgaatac ccagcgcgaa caggaagcgc gccaaggtcg tgcgcttgct
    10741 cgcctccagc ccggcgagca ggttggccgc actcttgtct cccatgcgat ccagcgcgcg
    10801 cagcttggcg aggtccagcg tgtagagctc gggaagtgtg cggatcaggc ccgcatcgac
    10861 gagctggtcg accagcttgt cacccagtcc ctcgatgtcc atcgcgcggc ggccggcgaa
    10921 atgcaggatc gcctccttgc gctgcgccgg gcaggtcaac ccaccggtgc agcgccagtc
    10981 gacctctccc tcctcgcgcc cgatcgggct gccgcagacc gggcacgcgc ctccgagccg
    11041 ctggtacagg tcgaacggct cgccacgcag cgccgcatcc gcagaccccg tcgtgtcgtg
    11101 caccacgccg accacctgcg gaatcacatc gccggcacgc cgcacgatca ccgtgtcgcc
    11161 ggcacgcacg tccttgcgcc gcacttcgtc ctcgttgtgc agcgtcgcgt tgctgaccgt
    11221 cgtgccgcca acgaagacgg gctccagcct ggccaccggc gtcagcttgc cggtgcggcc
    11281 gacctggatc tcgatgccca gcagcttcgt catctgctcc tgggccgggt acttgtgcgc
    11341 caccgcccag cgcggctcgc gcg
//
LOCUS       DAOORV010000113        11269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7223_length_11269_cov_7.210986, whole genome shotgun sequence.
ACCESSION   DAOORV010000113 DAOORV010000000
VERSION     DAOORV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7223_length_11269_cov_7.210986"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKV56_13585"
     CDS             complement(<1..1040)
                     /locus_tag="PKV56_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOX68890.1"
                     /translation="MTRLQDIAAQLSGYDPSALSVAKAQEFIARLVPRVTAVESLPLR
                     SALGRVLARDIVSPINVPASDNSAMDGYALRSSELRASGPSDFGVAGTSFAGEAFDGV
                     AAPGQCVRIMTGAVMPDRFDTVVPQEFVQLTADGTVTVPAGVVRSGDNRRLAGEDLRL
                     GEPALQAGRVLRPADLGLMASLGRAEVPVFRRLRVAFFSTGDELRSVGEALDEGCVYD
                     SNRYTLWGMLQRLGVELIDMGVVPDEPEALEATFRAAAENADAVITSGGVSVGEADHT
                     KQVMARIGDVLFWKIAMRPGRPMAIGRIESNGRQAILFGLPGNPVAVMVTFYAFVRDA
                     LLAMSGAASCPLP"
     gene            complement(1080..1682)
                     /gene="mobA"
                     /locus_tag="PKV56_13590"
     CDS             complement(1080..1682)
                     /gene="mobA"
                     /locus_tag="PKV56_13590"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HOX68891.1"
                     /translation="MIERAQITGLVLAGGRGSRMGGVDKGLQNHQGVPLALHAMLRLA
                     PQVGAMMINANRNLAAYESFGVPVWPDAIADYPGPLAGFLAGLEHCDTPYLVTVPCDT
                     PNFPADLVARLARELVEHDARIAMAATPLADGTQVQPVFCLMESSLMSSLIEFTQGGQ
                     RKIDRWTALHRCVDVVFDDAEAFANANTLQELQQLQAATR"
     gene            complement(1679..1969)
                     /locus_tag="PKV56_13595"
     CDS             complement(1679..1969)
                     /locus_tag="PKV56_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68892.1"
                     /translation="MGRLWSQVPSVVKRRLGWSAVACVGVPWLLATAAKTAFQPGIAN
                     DPDHMTRFIDILAISASVFALSMVCTVALGCVLVAAMKGPAYLGDEFPADRP"
     gene            complement(1971..3086)
                     /gene="moaA"
                     /locus_tag="PKV56_13600"
     CDS             complement(1971..3086)
                     /gene="moaA"
                     /locus_tag="PKV56_13600"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOX68893.1"
                     /translation="MSEKVIPLADHRYASAVPTVPDILRAPTGRLLDRRDRPLRDLRI
                     SVTDRCNFRCSYCMPKDVFDNRYDFLPQSSLLSFEEISRLAGLFVAHGVHKIRLTGGE
                     PLLRKGLENLIRMLGELRTPEGHPVDLTLTTNGSVLAKKAQALRDAGLQRITVSLDAL
                     DDTIFRRMNDVDFPVAEVLDGIEAAERAGLGPIKVNMVVKRGTNDSEIVPMAARFRNT
                     KVIPRFIEYMDVGATNGWRMDEVLPSADVVARIAAEFPLEPMEASERGETAQRWRYRD
                     GSGEFGVISSVTQAFCRDCNRARLSTEGKLFLCLFANQGHDLRALLRGSCTDEQIAAA
                     IGLIWNGRDDRYSELRSGLIATAESGARRVEMHYIGG"
     gene            complement(3169..3744)
                     /locus_tag="PKV56_13605"
     CDS             complement(3169..3744)
                     /locus_tag="PKV56_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOX68894.1"
                     /translation="MLAIFWQAQALAAPDIDGDAVTRLLIERGLVSESSGAAPADPSP
                     TLVQRVRDGASDMVIVAMNFLGVPYRRGGSSEADGFDCSGFTRHIFEMSLGLVLPRRV
                     DDQASASGLIEVTRDQLRPGDLVFFNTLRRTFSHVGIYVGDGKFIHSPRSGGAVRVED
                     MRFAYWSQRFTGARRAGAPAHAPTADPAAAR"
     gene            3954..4934
                     /locus_tag="PKV56_13610"
     CDS             3954..4934
                     /locus_tag="PKV56_13610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HOX68895.1"
                     /translation="MFKALLLEKTEAGFTAGVKLVDEAQLPEGDVLVAPSHSTLNYKD
                     GLAITQRGPVVRAWPMVAGIDGAGTVIESSHPRWQPGDRFIHNGWGVGESRWGCLAER
                     ARLKGDWLVKLPEAFTARQAMALGTAGYTAMLCVLALERHGVRPGDGEVLVTGATGGV
                     GSVATMLLSKIGHKVVAATGKSAEADYLMRLGATAVIDRAELSSPGKPLEKERWAAVV
                     DAVGSHTLANALAQTRYNGVVAACGLAQGADLPATVMPFILRGVTLAGVESVLVPLPV
                     RVQAWARLARDINLDLLEEMAEDIALGDCVARARDLIDGKVRGRLVVSIA"
     gene            4976..6871
                     /locus_tag="PKV56_13615"
     CDS             4976..6871
                     /locus_tag="PKV56_13615"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HOX68896.1"
                     /translation="MTRYTDFHQRSLTDRDGFWTEQAALIDWHRPFDQVCDHSNPPFA
                     RWFVGGQTNLCHNAVDRHLAQRGDQAALICVSTETDTEHTYSFRDLHAEVQRMAAILL
                     SLGVKQGDRVLVYMPMVPQAVFAMLACARIGAIHSVVFGGFASVSLASRIDDAQPTVI
                     VSADAGSRSGKVLPYKPLLDEAIRLAEHKPTKVLLVDRGLSPMDRVPGRDIDYAELRQ
                     QHLDAVVPCSWVESTHPSYTLYTSGTTGKPKGVQRDTGGYAVALAASMKHIYCGNPGE
                     TFFCGSDIGWVVGHSYIVYGPLINGMATILYEGLPTRPDAGIWWSLVEKYRVSVMFSS
                     PTAIRVLKKADPKYLAQYDLSTLRALFLAGEPLDEPTSKWIAESLGKPIVDNYWQTET
                     GWPILTIANGIEKSPAKFGSPGFAMYGYDVKVMDELTGEEVTAPNQKGVLVIEGPLPP
                     GCMQTVWRDDARFVKTYWSGKPGKQVYSTFDWGIRDEDGYFFILGRTDDVINVAGHRL
                     GTREIEESISSHPSVAEVAVVGVADALKGQVAMAFVVLKDASKAATEADAASLEAEVM
                     KVVDKQLGALARPARVRFVGALPKTRSGKLLRRAIQAVCEQRDPGDLTTIDDPSSLQQ
                     IRESISG"
     gene            6970..7851
                     /locus_tag="PKV56_13620"
     CDS             6970..7851
                     /locus_tag="PKV56_13620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOX68897.1"
                     /translation="MNSFDRITRRHVLVTLAMPWPALRCLEAPAAPSESPPPAVSLLT
                     EAPADIDPIGYLVSEKLDGVRALWDGSELRFRSGRRIEAPAWFTEALPATALDGELWS
                     GRGRFDFVSGAVRRNTPDDALWRSVRFMIFELPGSAGDFALRAARIADHVRMSSAQFL
                     GAVEQAVVPDRASLRSRLAAVVRGGGEGLVLHRSDAPWKPGRDRSVLKLKPQQDAEAQ
                     VVAYEPGRGRLTGLMGALQVRNEDGVAFRIGSGFADAQRRDPPPIGSWITYTHRGHTP
                     GGVPRFASYWRARDTDL"
     gene            complement(7856..9529)
                     /locus_tag="PKV56_13625"
     CDS             complement(7856..9529)
                     /locus_tag="PKV56_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOX68898.1"
                     /translation="MPLPKPPAKRASLLSVTDFESQAAPKPARRSPRTSAAAEPAAPA
                     ELSLYDPAALAPTPPTRAPMAAESLDTSAALIKRAEPKPRASKPRGTGPAKLFVLDTN
                     VLMHDPMSLFRFDEHDIYLPMITLEELDGHKKGMTEVARNARQVSRDLDALAASMATR
                     DINGMTEGLPLSRTGHREAGGKLFFQTNLVNVTLPAGLPQGKADNQILGVVQALREQQ
                     PGRDVVLVSKDINMRIKARALGLPAEDYFNDKTLEDGDLLYTGVLQLPSDFWDRHGKT
                     MESWNQGGFTFYRISGPMVPALLINQFVYLEAPGAAPLHAKVTEITGKTAVLRTLREY
                     THAKNAVWGVTARNREQNFALNLLMDPECDFITLTGTAGTGKTLMTLAAGLSQVMDDR
                     RYTEIIVTRVTVPVGEDIGFLPGTEEEKMGPWMGALDDNLEVLSKTDGGAGEWGRAAT
                     NDLVRSKIKIKSLNFMRGRTFLNKFVLIDEAQNLTPKQMKTLITRAGPGTKIVCLGNL
                     AQIDTPYLTEGSSGLTYAVDRFKGWPHSGHVTLARGERSRLADFASEVL"
     gene            complement(9671..10147)
                     /locus_tag="PKV56_13630"
     CDS             complement(9671..10147)
                     /locus_tag="PKV56_13630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOX68899.1"
                     /translation="MTPALNKPLPEFEALATSGVKFTPQAYAGQSIVLYFYPKDNTPG
                     CTTEAMQFRDHHKDFIAAGAVVFGVSRDNMTSHEKFRQNLELPFDLIADTEEKLCHMF
                     GVVKNKIMYGKKVKGIERSTFLIDGAGVLRAEWRGLKVAGHVEEVLKTAKSLKGEA"
     gene            complement(10246..10620)
                     /locus_tag="PKV56_13635"
     CDS             complement(10246..10620)
                     /locus_tag="PKV56_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOX68900.1"
                     /translation="MKLNAERIEGTNAIARHGKDGVMVNGVQFTSSVVVPWTGLVTLW
                     PVARFADLNTEHFAALAQHEPELILFGSGEKLRFPPASLLRTLIDRRIGFETMDTAAA
                     CRTYNVLLSEGRNVVAGLLFEH"
     gene            10695..>11269
                     /locus_tag="PKV56_13640"
     CDS             10695..>11269
                     /locus_tag="PKV56_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX68901.1"
                     /translation="MKAVAKSSKLANVCYDIRGPVLDKARQMEEEGQKIIKLNIGNLA
                     VFGLEPPDEIVQDMIRNLPHSAGYTDSKGLFAPRKAVVHYMQEKHVHGVTVDDVYLGN
                     GASELITMSMNGLLNDGDEVLVPAPDYPLWTASVALSGGTPVHYICDEQADWTPDIDD
                     IKRKITPNTKAIVVDVRRPVRLLVADVVDAG"
BASE COUNT         1944 a         3630 c         3743 g         1952 t
ORIGIN      
        1 ggcagcgggc aggacgcggc gccgctcatc gccagcagcg catcgcgcac gaaggcatag
       61 aaggtcacca tcaccgccac cgggttcccc ggcagcccga acaggatggc ctgccggccg
      121 ttgctctcga tgcggccgat cgccatcggt cggccggggc gcatcgcgat cttccagaac
      181 agcacgtcgc cgatccgcgc catcacctgc ttggtgtgat cggcctcgcc cacgctgacg
      241 ccgcccgagg tgatcaccgc atcggcattc tcggccgcag cccggaacgt cgcctccagg
      301 gcctccggct cgtcggggac cacgcccatg tcgatcagct cgacgccgag ccgctgcagc
      361 atgccccaca gcgtgtagcg gttgctgtcg tacacgcagc cctcatcgag cgcttcaccg
      421 accgagcgca actcgtcgcc ggtcgagaag aaggccacgc gcaggcgcct gaacaccggt
      481 acctcggcgc gacccagcga ggccatcaat ccgagatcgg ccggacgcag caccctgccg
      541 gcctgcagtg cgggctcgcc gaggcgcagg tcttcaccgg ccaggcggcg gttgtcgccg
      601 gatcgcacga cgcccgctgg cacggtcacc gtgccgtctg ccgtcaactg aacgaattcc
      661 tgcggaacga ccgtgtcgaa cctgtcgggc atgaccgcac ccgtcatgat gcgcacgcac
      721 tggccagggg ccgccacacc gtcgaaagcc tcgccggcga aactggtgcc ggcgacaccg
      781 aagtcactgg gaccgctggc cctgagctcg ctcgatcgca gcgcgtagcc gtccatggcc
      841 gagttgtcgc tggccggcac gttgatcggg gagacgatat cgcgggccag cacgcggccg
      901 agtgcgctgc gcagcgggag gctctctaca gccgtgaccc gaggcaccag tcgggcgatg
      961 aactcctgtg ccttggctac cgatagcgcg ctcgggtcgt agccgctcag ctgtgcggcg
     1021 atgtcttgca gtcgtgtcat ggcggattca ctgggcaggg ggcaggcagg tgttggcaat
     1081 cagcgcgtgg ccgcctgaag ctgctgcagt tcctggagcg tgttggcgtt ggcaaaggct
     1141 tctgcgtcgt cgaaaaccac gtcgacgcag cggtgcaggg cggtccatcg gtcgatcttg
     1201 cgctggccac cctgcgtgaa ctcgatcagg ctggacatca ggctggattc catcaggcag
     1261 aacacgggct gcacctgcgt gccgtcggcg agaggcgtag cggccatcgc gatgcgtgca
     1321 tcgtgctcaa ccagctcgcg ggcgagtcgt gcgaccagat cggctggaaa attcggcgtg
     1381 tcgcacggca cggtcaccag gtacggtgtg tcgcagtgtt ccagtccggc caggaatcct
     1441 gccaacggcc ccgggtagtc tgcgatcgca tccggccaca ccggcacgcc gaaggattcg
     1501 taggcagcca ggttgcgatt ggcattgatc atcatcgcgc cgacctgcgg cgcaaggcgc
     1561 aacatcgcat gcagtgccaa cggcacgcct tgatgattct gcagaccctt gtcgacgccc
     1621 cccatccggc tgccgcgtcc tccggccagg acgaggccgg tgatctgtgc gcgctcgatc
     1681 atggccggtc ggcagggaat tcgtcgccga gatacgccgg acccttcatg gcggcgacga
     1741 gcacgcagcc cagcgcgacc gtgcacacca tcgacagtgc gaacacgctg gcgctgatgg
     1801 cgaggatgtc gatgaaacgc gtcatgtggt ccgggtcgtt tgcgattcct ggctgaaatg
     1861 ccgtcttggc cgcggtcgcg agcaaccatg gcacgcccac gcaggccacc gcggaccagc
     1921 cgagtcgccg cttgacaacg ctgggaacct gactccacaa gcggcccatg tcaacctccg
     1981 atgtagtgca tctcgacgcg tctggcgcca gactcggctg tggcgatcag gccgctgcgc
     2041 agttcggaat agcggtcgtc acggccgttc cagatcaatc cgatggcggc ggcgatctgc
     2101 tcgtcggtgc aggagccccg cagcaacgcg cgcaggtcgt gcccctggtt cgcgaacagg
     2161 cacaggaaca gctttccttc ggtcgacagc ctcgcgcggt tgcagtcgcg gcagaaagcc
     2221 tgtgtgacgc tcgagatcac gccgaactcg ccgctgccgt cgcgatagcg ccatcgctgc
     2281 gcggtctcgc cccgctcgct tgcctccatc ggctcaagcg gaaactcggc agcgatgcgc
     2341 gccacgacgt ctgccgacgg cagtacctcg tccatgcgcc agccattggt tgcgcccacg
     2401 tccatgtact cgatgaaacg cgggatgacc ttcgtgttcc ggaagcgtgc ggccatcggc
     2461 acgatctcgg agtcgttggt gcctcgcttg acgaccatgt tgaccttgat cggaccgagc
     2521 ccggctcgct cggcagcctc gatgccatcg agcacttcgg cgaccgggaa atccacgtcg
     2581 ttcatgcgcc gaaagatggt gtcgtcgagt gcatcgaggc tgacggtgat gcgctgcaaa
     2641 cctgcatcgc gcagggcctg tgccttcttt gcaagtacag agccgttggt tgtcagcgtc
     2701 aggtcgaccg ggtgtccctc tggcgtgcgc aattcgccga gcatgcgaat caggttctcg
     2761 agacccttgc gcagcagcgg ctcgccaccg gtcaaccgga tcttgtgcac cccgtgtgcg
     2821 acgaacaagc cggcaagccg cgaaatttcc tcgaagctca gcaacgagct ctgcggcagg
     2881 aagtcgtatc ggttgtcgaa gacatccttc ggcatgcagt agctgcagcg gaaattgcat
     2941 cggtcggtga ccgagatgcg caggtcgcgc aggggacggt cccgtcggtc gagcagccgt
     3001 ccggtcggtg cacgcaggat gtccggcacg gtcgggacgg cagacgcata ccggtggtcc
     3061 gccagcggaa tcactttctc agacatgctc gaattgtgct cgctggacat tgaccttgtg
     3121 ccggcgggac acgctgcctg accgcgaccc tgccggcgcc gtcaggggtc agcgtgccgc
     3181 cgctggatcg gcggttggcg catgggctgg ggcgccggcc cggcgtgcgc cggtgaaccg
     3241 ctggctccag taggcaaagc gcatgtcctc gacgcgcact gcgccgccgc tgcgagggga
     3301 gtggatgaac ttgccgtcgc ccacgtagat gccgacatgc gagaacgtcc gtcgcagcgt
     3361 gttgaagaac accagatcgc cgggccgcaa ctgatcgcgg gtcacctcga tcagcccgga
     3421 ggcgctcgcc tggtcatcga cccgacgcgg cagcaccagt ccgaggctca tctcgaagat
     3481 gtggcgcgtg aacccgctgc aatcgaaacc atctgcctcg ctggaccctc cgcggcgata
     3541 cgggacgccg aggaaattca ttgcgacgat gaccatgtcg gaggctccgt cgcgcacccg
     3601 ctgaaccagc gtcggagatg gatccgccgg cgccgcaccg ctgctttcgg agaccagacc
     3661 gcgctcgatc aacagccgcg tcaccgcgtc gccgtcgatg tccggcgctg ccagcgcctg
     3721 cgcttgccag aaaatcgcca acatgccgaa gctggtcacc agtgccatgt cgcaggcgcg
     3781 atccgcgcga cgacggggcg gggcgcatgg gtgggctggc ttcggcatcg gggcaggata
     3841 ggtgacgctc cgaggcccgt caattgcacc taagtggtgc aatttcaggt cgtcagacgc
     3901 ccgacagcaa catgggcgat gctcgcagac cggatcaacg cagaggaact ccgatgttca
     3961 aggcactcct gctcgagaag accgaagcgg gtttcaccgc cggggtgaag ctggtcgatg
     4021 aggctcagct gcccgaaggg gatgtgctcg ttgccccttc gcattcgacg ctcaattaca
     4081 aggacggcct cgccatcacg cagcgtggac ctgtggtgcg tgcctggccg atggtggccg
     4141 gcatcgatgg ggcgggaacg gtgatcgaaa gcagccaccc ccgatggcag ccgggcgacc
     4201 gcttcatcca caacggctgg ggcgtcggtg aatcccgctg gggctgcctg gccgagcggg
     4261 cgagattgaa gggtgactgg ctggtcaagc tgccagaagc cttcacggca cggcaggcga
     4321 tggcattggg cactgccggc tacaccgcca tgctgtgcgt gctggcgctt gaacgccacg
     4381 gcgtgcggcc aggcgacgga gaggtgctgg tgaccggtgc caccgggggt gtcggcagcg
     4441 tggccacgat gctgctgtcg aagatcgggc acaaggtggt tgctgccacc ggaaagtcgg
     4501 ccgaggccga ttacctgatg cggctgggcg cgaccgcggt gatcgaccgc gccgaactgt
     4561 cgtctcccgg caagccgctg gagaaggagc gctgggcggc agtcgtcgat gccgtgggca
     4621 gccacacgct ggccaacgcg ctggctcaga cgcgctacaa cggcgtggtg gctgcctgcg
     4681 ggctggcgca gggcgccgat ctgccggcca cggtgatgcc tttcatcctg cgcggcgtca
     4741 cactcgctgg agtcgaaagc gtgctcgtac cgctgcctgt gcgagtgcag gcctgggcac
     4801 ggctggccag ggacatcaac ttggatctgc ttgaagagat ggccgaagac atcgccctgg
     4861 gagactgcgt ggcacgagcc cgggacctga tcgacggcaa ggtgagagga cggctggtgg
     4921 tttcgatcgc ctgaagccag accgtccatc caacaagaaa ctacggagac gaaccatgac
     4981 gcgctacacg gatttccatc aacgctcgct gaccgaccgc gatggcttct ggaccgaaca
     5041 ggccgcgctg atcgactggc atcgaccttt cgaccaggtc tgcgatcaca gcaatcctcc
     5101 gtttgcgcgc tggttcgtgg gcggccagac caacctgtgc cacaacgccg tcgaccgcca
     5161 tctggcgcag cggggcgacc aggcggcgct gatctgcgtg tcgaccgaaa ccgacaccga
     5221 acacacctac agcttccgtg acctgcatgc cgaggtccag cgcatggcgg cgatcctgct
     5281 ttcactgggg gtgaagcagg gcgatcgcgt gctggtctac atgccgatgg tcccgcaggc
     5341 ggtgtttgcg atgctggcct gtgcgcgcat cggcgccatc cactcggtgg tgttcggagg
     5401 gttcgcgagc gtcagcctgg ccagccgcat cgacgatgcg cagccaacgg tgatcgtcag
     5461 cgccgacgcg ggcagccgct ccggcaaggt gctgccctac aagccgctgc tcgacgaggc
     5521 catccggctg gctgagcaca agccgaccaa agtgctactg gtcgaccgcg ggctcagccc
     5581 gatggaccgg gtgcccggac gcgacatcga ttacgccgaa ctgcgccagc aacacctcga
     5641 tgccgtggtg ccgtgcagct gggtcgaatc cacccacccc agctacacgc tgtacaccag
     5701 cggtacaacc ggcaagccca agggcgtgca gcgcgacacc ggcggctacg cggtggcgct
     5761 ggccgccagc atgaagcaca tctattgcgg caatcccgga gaaaccttct tctgcggcag
     5821 cgacatcggc tgggtcgtcg gtcacagcta catcgtctac ggcccgctga tcaacggcat
     5881 ggcgaccatc ctctacgagg gtctgccgac gcgaccggac gccggcatct ggtggagtct
     5941 ggtcgagaag taccgtgttt cggtcatgtt cagttccccg accgcgatcc gcgttctgaa
     6001 gaaagccgac cccaagtacc tcgcgcagta cgacctgtcg acattgcgtg cgttgttcct
     6061 ggccggcgag ccgctggacg agccgacctc gaagtggatc gcggagtcgc tgggcaagcc
     6121 catcgtggac aactactggc agacggaaac cggctggccg atcctgacca ttgccaatgg
     6181 catcgagaag tctcctgcca agttcggcag tcccggcttc gcgatgtacg gctacgacgt
     6241 caaggtgatg gacgaactga cgggagagga agtcaccgcg cccaatcaga agggtgtgct
     6301 ggtcatcgag ggtccgctgc cgccgggctg catgcagacc gtctggcgcg acgacgcccg
     6361 attcgtcaag acctactgga gcggcaagcc tggcaagcag gtctacagca cgttcgactg
     6421 gggcatccgt gacgaggatg gctacttctt catcctcggc cgtaccgacg acgtgatcaa
     6481 cgttgcgggc caccgcctgg gcacccgcga gatcgaggag agcatttcca gccacccgag
     6541 cgtggccgag gtcgctgtgg tcggcgtggc cgatgcgctc aagggccagg tcgcgatggc
     6601 gttcgtggtg ctgaaggacg cgtctaaggc ggccacggag gccgacgccg ccagcctcga
     6661 agccgaggtc atgaaagtgg tcgacaagca gctcggcgcc ctggcccggc ccgcgcgtgt
     6721 gcggttcgtc ggggccctgc ccaagacgcg ttcgggcaag ctgctgcggc gcgcgatcca
     6781 ggccgtgtgc gaacagcgcg acccggggga cctgaccacg atcgacgacc cgagctcgct
     6841 ccagcagatc agggaatcga tctccggctg agatcgctcg caattcggtg ggtccgtcca
     6901 tcctccgggg gatgacggcc cgccggccgc tgcacagact ggtgctccaa ccatcaccgg
     6961 agcagcagca tgaactcgtt cgaccgcatc acacgccggc atgtcctggt gaccttggcc
     7021 atgccgtggc ctgcactgcg ctgcctggag gcgccggctg cgccctcgga atcgccgcca
     7081 ccggccgtgt cactgctgac cgaagcgccg gcagacatcg acccgatcgg ctacctcgtc
     7141 agcgaaaagc tcgatggggt gagggccctc tgggacggca gcgagctgcg ctttcgcagc
     7201 ggacggcgga ttgaggcgcc ggcatggttc accgaggcac tgccggcgac tgctctcgat
     7261 ggcgaactgt ggagcggccg tggtcgcttc gatttcgtat ccggagcggt acgccggaac
     7321 acgcccgacg atgcgctgtg gcgctcggtg cgattcatga tcttcgagct tcccggatcc
     7381 gcaggcgatt tcgccctgcg cgcggcgcgc attgcagacc acgtgcggat gtcgtcagcg
     7441 cagtttctcg gggccgtcga gcaagcggtg gtccccgacc gcgcctcctt gcgcagtcgc
     7501 ctcgccgcgg tggtgcgggg cggcggtgaa ggcctggtgc tgcatcgttc ggatgcgccc
     7561 tggaagccgg ggagagaccg gtctgtgctg aagctcaagc cgcagcagga tgccgaagcc
     7621 caggtcgtgg cgtacgagcc cggacgcggg cggctgacgg ggctgatggg tgccctgcag
     7681 gtgcgaaatg aggatggcgt cgcgttccgc atcggaagcg gcttcgccga cgcgcagcgc
     7741 cgggatcccc cgccgattgg tagctggatc acctacaccc accggggtca cacgccgggc
     7801 ggagttccgc gcttcgccag ctactggcga gcgcgcgata ccgacctgtg actcgtcaga
     7861 gcacctcgct ggcgaaatcg gcgagtctcg aacgctcgcc ccgagccagg gtcacgtggc
     7921 cgctgtgcgg ccagcccttg aaccggtcca cggcataggt cagtcccgaa ctgccttcag
     7981 tgaggtacgg cgtgtcgatc tgcgccaggt tgcccagaca gacgatcttg gtgcccgggc
     8041 cggcgcgggt gatcagcgtc ttcatctgct tcggcgtcag gttctgggcc tcgtcgatca
     8101 gcacgaactt gttcaggaag gtgcggccac gcatgaagtt caggctcttg atcttgatct
     8161 tgctgcgaac caggtcgttg gtcgccgcgc ggccccattc gccggcgccg ccatcggtct
     8221 tggaaagcac ctcgaggttg tcgtcgagcg cgcccatcca ggggcccatc ttctcttcct
     8281 ccgtgccagg caggaagccg atgtcctcgc ccaccggcac ggtgacgcgt gtgacgatga
     8341 tctccgtgta gcggcggtcg tccatcacct gactcagccc ggcagccaga gtcatcagcg
     8401 tcttgccggt tcccgcggtg cccgtcagcg tgatgaagtc gcactccggg tccatcagca
     8461 ggttcaatgc aaagttctgc tcgcgattgc gcgcggtcac gccccacacc gcgttcttgg
     8521 cgtgggtgta ttcgcgcagc gtgcgcagca ccgccgtctt gcctgtgatc tcggtgacct
     8581 tcgcatgcag tggcgcggcc cccggcgcct cgaggtagac gaactggttg atcagcaagg
     8641 caggcaccat gggcccgctg atccggtaga aggtgaagcc gccctggttc cagctctcca
     8701 tggtcttgcc gtgacggtcc cagaagtcgc tgggcagttg cagcactccg gtgtagagca
     8761 ggtccccgtc ctcgagcgtc ttgtcgttga agtaatcctc cgcaggcagc cccagtgccc
     8821 gagccttgat gcgcatgttg atgtccttgg acaccagcac cacatcccgc ccgggttgct
     8881 gctcgcgcag tgcctgcacc acaccgagga tctggttgtc ggccttgccc tgaggcaggc
     8941 cggccggcaa cgtcacattc accaggttgg tctggaaaaa cagcttgccg cccgcctcgc
     9001 gatgcccggt gcgcgacagc ggcagtccct cggtcatgcc attgatgtcg cgcgtggcca
     9061 tgcttgcggc cagcgcatcg aggtcgcggc tgacctggcg cgcattgcgt gcgacctcgg
     9121 tcatcccctt cttgtgtcca tcaagctcct cgagcgtgat catcggcagg tagatgtcgt
     9181 gctcgtcgaa gcggaagagg ctcatcgggt cgtgcatcag cacgttcgtg tcgagcacga
     9241 acagcttggc cgggccggtg cctctcggct tggacgcgcg cggcttcggt tcggcacgct
     9301 tgatcagcgc ggcactggta tccagcgact cagccgccat cggagcacgc gttggaggtg
     9361 tcggtgcaag cgccgccggg tcatagaggc tcaactcggc cggcgcagcc ggctccgctg
     9421 ccgcgctggt gcgtggcgaa cgcctggcgg gcttgggtgc tgcctgggat tcgaagtcgg
     9481 tgacggacaa cagggaggcg cgtttggcag gcggcttggg cagtggcatg agggcggagg
     9541 atgatgagtg acaggcggga ggtgacagga ctcaggctgg aaaacaaaga agccgcgcag
     9601 gttcctgtgc ggcttccatt tgaacagggc gacgtgacgc gctggtacct gtatgcaatc
     9661 tttgaaccaa tcaggcttca cccttcaggg atttggccgt cttgagaact tcctcgacgt
     9721 ggccggcgac cttgagacct ctccattcgg cgcgcagcac gcctgcgccg tcgatcagga
     9781 aggtactgcg ctcaatgccc ttgaccttct tgccgtacat gatcttgttc ttgaccacgc
     9841 cgaacatgtg gcaaagcttc tcctcggtat cggcaatcag gtcgaacggg agttccaggt
     9901 tctgcctgaa cttctcgtgt gaagtcatgt tgtcgcggga aaccccgaaa accacggcac
     9961 cggcggcaat gaaatccttg tggtgatcgc gaaactgcat ggcctcggtg gtgcagccgg
    10021 gggtgttgtc cttcgggtag aagtacagga cgatcgactg gccggcgtag gcctgcgggg
    10081 tgaacttcac tccactggtg gcgagcgctt caaattccgg gaggggtttg ttgagggctg
    10141 gcgtcatgga gtcttgtagg tgtcgcgccg tcgttggcgt agcccacaat tataggggct
    10201 gaccacgggc cctctcagac ggctacacgc gcccttggcg ttgcttcagt gctcgaacag
    10261 caggccggcc acgacgttgc ggccctcgga gagaagcacg ttgtaggtgc ggcaggcggc
    10321 tgcggtatcc atcgtctcga aaccgatgcg ccggtcgatc agggttcgca acagactggc
    10381 aggtggaaag cgcagcttct cgccgctgcc gaacagaatg agttcgggct cgtgctgcgc
    10441 cagcgcggcg aaatgctcgg tgttcaggtc ggcaaagcga gccaccggcc acagcgtgac
    10501 gagtccagtc catggcacca cgacgctcga agtgaactgg accccgttga ccatcacacc
    10561 gtccttgcca tggcgcgcga tcgcgttggt accttcgatc cgttcggcgt tcagtttcaa
    10621 ggtcgcgttc tcactgtgtc tagagcgtaa aattgtaggc tttgcgccgt tctggcgcga
    10681 tgccggagag ccctttgaaa gctgtcgcca agtcgagcaa gctcgccaac gtctgctacg
    10741 acatccgtgg tccggtgctc gacaaggccc ggcagatgga ggaagaaggg cagaagatca
    10801 tcaagctgaa catcggcaat ctcgccgtgt tcgggctcga gccgcccgac gagatcgtgc
    10861 aggacatgat ccgcaacctg ccgcacagcg ccggctacac cgacagcaag ggcctgtttg
    10921 ctcctcgcaa ggcggtggtg cactacatgc aggaaaagca cgtccacggc gtgaccgtcg
    10981 atgacgtgta tctcggcaac ggggcctccg aactgatcac gatgagcatg aacgggctgc
    11041 tcaacgatgg cgacgaagtg ctggtgccgg cacccgacta tcccctgtgg actgcttcgg
    11101 tcgcgctgtc cggcggcacg ccggtccact acatctgcga cgagcaggcg gactggacgc
    11161 cggacatcga cgacatcaag aggaagatca ctccgaacac caaggcgatc gtcgtcgatg
    11221 tccggcgtcc agtccgcctg ctcgtcgcag atgtagtgga cgccggaca
//
LOCUS       DAOORV010000114        11252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7235_length_11252_cov_8.892471, whole genome shotgun sequence.
ACCESSION   DAOORV010000114 DAOORV010000000
VERSION     DAOORV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_11252_cov_8.892471"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKV56_13645"
     CDS             complement(<1..692)
                     /locus_tag="PKV56_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68902.1"
                     /translation="MSQPWALSPHIDLRSDRVPERDAWRQMHTAAEICRRLNSQPGVV
                     LADDVGMGKTFVALAVAASIVQQDPTRQIVVMVPTSVGDKWPKDWSVFQARCLHGGPP
                     IRATGHTVRRGAEFLKLLDEPPETRRQIIFLSHWALSTNLDDPFIKLALVRTAFRYQR
                     DLQPQRQALPRWAQRLFNRSDFSEAAVEALLAAEPGRWRGVWQKQTGKDLQDDPVPTF
                     LIEAFNRCDLREV"
     gene            complement(689..2710)
                     /locus_tag="PKV56_13650"
     CDS             complement(689..2710)
                     /locus_tag="PKV56_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOX68903.1"
                     /translation="MKMLDNWLSKDGAGLPLACLATTFTFDADFFAEDCLSRFLSISA
                     RDPDGATGLDIAGMLEEEERLAETQVCVLVDRSCRPDARNLRWDLVPVRVPHGLLHAK
                     VVVLIWERSMRVVIGSANLTRAGYRHQVELGMAIDVDDDCAVPKAVFEDLLRELRGIL
                     ALAPGALDRAGPKQRAERILQRFESRLSSLSFKPSASQGLKFALAAGSRDSRPLDRLA
                     DVWSGSPPQTVVAMSPFWDDTPDMAGAKEVLERLAKRAPAGARTRATFVVPVDTTAVG
                     TIVRAPVQLRSVAGSRIQARVASFAAQDDRRLHAKCIQYQSATWFAVMFGSSNITAKG
                     LGLDRAPHRELNLWLGCRVDSPHAKKLATLIPVGEELDGGLEFEPASDDEDESAVADL
                     PDGFEEALLMRRECIELSFRTDRLPESWTLGWTPPGQVRVHLLDATAWRGHGQPVRME
                     VSLPANLEVLPFLLDVDWTAAGESNRAGWIVNIGPEAVLPPPSELTGLSSDVLLAILA
                     STRPLREAIENALRAKSTRKKPGGDELDPLKRFDDSGLLIQRVRRASAALWGIARRLS
                     ARCSSLDALEWRLAGTLGPEHVARKLADAASCDGALAGEAQFLIGELALTVHRVPWGT
                     LIVDLPEQVVHRRIAQTLGVLKGIVDGLNTTRHPAIDTYTQNVFQEVGA"
     gene            complement(2707..4011)
                     /locus_tag="PKV56_13655"
     CDS             complement(2707..4011)
                     /locus_tag="PKV56_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68904.1"
                     /translation="MNLTLPQLSDADPTISGEGSLDPLGMAQLADRLAESLLPGLRAR
                     MRRIRFLTASAVGALAAESLVDVLPVDRISTPTICFEWLVLEAFARRSGKDLPLEASG
                     IPGSSKVRSVLAQGKRLAARNYLKSPNVFGFTGIYLPLARHLQVLDEDRRVAARLSDL
                     TQAWERDRGLSGYTDGKPGTDGGRLRLRLRDEVRAALQHGHCAAPEGSHLWRQLSEAL
                     HPTQAGRAEKALLRHWIVDDNMPMRAELASFLDQQGDASEADVLKSLLAGQRSLDLRL
                     RLEAISAYEAVAHLLGASFAQVCHLSTMLGTRPLTAADVSRDQVLNDARARLPDAMKR
                     AADCLERIDGELLVVFSHRLGKFEQPGSTSDLVDLLMTHHEAVQNQKPPKGKRPWLDR
                     HAGGWVVRSIYWRSEAAQLVPGTLVHPYRLSALRGFMKDLAP"
     gene            complement(4133..>6112)
                     /gene="recD"
                     /locus_tag="PKV56_13660"
     CDS             complement(4133..>6112)
                     /gene="recD"
                     /locus_tag="PKV56_13660"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOX68905.1"
                     /translation="PANPLLVLAAALASHQLGRGHACLDLGATLADVRDALSLPPEPP
                     AAANVGAAATPRSPAALLAGVDLATWQQALHHPRLVGDGSGNTPLVLCGARLYLRRYW
                     QYEQTVREAIKQRLDQVIPPDEPALRQALQVLFPQVPAAQGTDWQKVACALAARHGFA
                     VITGGPGTGKTTTVVKLLAVLQHLAMNASPTARRLRIRMAAPTGKAAARLNESIAGAV
                     QRLPLDGLPDAEALRVAIPTEVTTVHRLLGSRPDTRHFRHDVRNPLPVDVLVIDEASM
                     VDLEMMASVVAALPAQARLVLLGDKDQLASVEAGAVLGDLCRRADKGHYTAATRAWVQ
                     QVANETVELALNDDQGLPLDQTIVKLRFSHRFDATSGIGRLADAVNAGSMSGLRTVRD
                     VVPPDLAWLNLAADSQALARLALDGACGAFAGQGQGRIDRGEPVAPPVGYRHYLERLR
                     ARRPPADADAEAWDAWAREVLVAYGQFQVLCAVRQGAHGVEGLNQRIADLLCVEGLTS
                     AAQGWYEGRPVMVTRNDYALGLMNGDVGITLQVPQRRGADPAATGAILRVAFAAGDGS
                     NSIHWVLPSRLQAVETVYAMTVHKSQGSEFTHAALVLPETPSRVLTRELIYTGITRAR
                     HWFTMATSDGGRGVLDLAVERRVKRTGGLLGHGEE"
     assembly_gap    6113..6212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6213..9835)
                     /gene="recB"
                     /locus_tag="PKV56_13665"
     CDS             complement(<6213..9835)
                     /gene="recB"
                     /locus_tag="PKV56_13665"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HOX68906.1"
                     /translation="MTTTLPPLLQPLRLCLRGSSLIEASAGTGKTFTIAALYLRLVLG
                     HGGTAAFTRPLTPPEVLVVTFTDAATQELRDRIRARLAEAAEAFALDPAEVPDRPRGE
                     DLLHDLRAEHAPALWPGCARTLRLAAEAMDESAVSTIHGWCNRMLREHAFDSGSLFTQ
                     ELQTDERDLLAEAVRDYWRSFYYPLAAVDVAQLRTWWKSPDDLGQLVGELLDLGDALP
                     DAEPPALALDAVRNEAGAELAIIKAPWKQWVGELRSMLDRAVASKQVNGRKLQARYYQ
                     PWLEALQEWVDGAAVLPLPDGSSGWTRLTPEGLTDAWAKGAPPDHPALLDMLTLRERI
                     QALPDGRTELLRHAACWIAARLADEKQRRAQMGFHDLLSRLDAALQGPNGARLAGLIR
                     QQFPVALIDEFQDTDPLQYRIFNAIYGVAGNDPQTALVLIGDPKQAIYAFRGADIHTY
                     LAARRDTAGRHSTLGTNFRSTGAMVAAVNQVFRQAEQRPGPGAFLFRNGDDDPVPFIE
                     VAARGRDERWCIDGAKAPALSFWQLDAGDPISATAYQQQMAAICATEVVRLLRAGQQR
                     RAGFWAGDGTMRPVQPGDIAVLVNTGREAQALRRALGEREVRSVYLSDKESVYASAVA
                     GDLQHWLAACAEPDDDRLLRAAIGTATLGLSWAELDRLNHDELHWEERVLQFRGYRQV
                     WRRQGVLPMLRRLLHDFEVPRGLLARHDERALTDVLHLAELMQQASGQLDGEHALIRH
                     LAEQRADEGGDSDIRKLRLESDADLVKVVTVHKSKGLEYPLVFLPFASAYRAAKDTDL
                     PLKWHDDQGRPRVDLKADAAVLACVDRERLGEDLRKLYVALTRARFATWVGVAPLKQL
                     ERGALGSLLSGGEAIEAGQLAAHLQALRGDCQAIAVLTAPTVAPDTWQPEAMSAQPRP
                     EPPLPAAPPEPWWIASYSALLATGPGRGESGGAVDLTAGDDASAAGVMPVTLPDSGAR
                     SAAEDIYAESQADAPLTLATAPVASAAPDAGAHTVAGGLHPFPRGAGAGSFLHGLLEW
                     AGRQGFARVRDEPGEVADLIARRCNLGGFSRWTQPLRDWLGHWLRTPWPLQQHLPGAS
                     PVCPTELGAIQVEMEFWFPAAQVNTRALDALVSRHTLDGAPRVPLQPNQLNGMLKGFI
                     DLVFEHEGRYFVADYKSNWLGPDDAHYTPQAMRDAVLHHRYELQYTLYLFALHRLLRS
                     RLP"
     gene            complement(9832..>11252)
                     /locus_tag="PKV56_13670"
     CDS             complement(9832..>11252)
                     /locus_tag="PKV56_13670"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF037683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOX68907.1"
                     /translation="RPGDRSRREDDRYLFLEALLSARERLHLSWVGRSIRDNQERPPS
                     VLVSQLRDHLAAGWRQAGDDQPQAKAGAALLTALTVAHRLQPFNPAYFNQGGDERLFS
                     YAREWRAVEAGRTQADGVGLATAAAHLPDGGEPGPLTLKLVSDFVKNPVRSFFQRRLG
                     IRFETDDPVGADQEPFAIDALENWQLQDELIQVQKAALVAGGSRQAALDAQLDRIALR
                     GELPAGEFGKLVQGELAEPMQAMFDAYAQALADWPQGCPDEPLDHLPEGFPEAQRLVD
                     WLGGMRRSTGGERGRVLLDSGSLVKENKYRHDKLLPFWVAHLAGHLAGEPLTTVVVSK
                     AGQVSFGPMDTLQARQRWQALLEAWQVGHRSPLPLALGCGFVWLGKGGHAGMSPHAAD
                     AFEAARVKYEDHEPKFNQFGERDGDAYLARAFPDFQALWAGGVFATWADALLRPLMDA
                     MPHKKREAKGDASKALATGDAE"
BASE COUNT         1774 a         3915 c         3731 g         1732 t
ORIGIN      
        1 acctcgcgca ggtcgcaccg gttgaaggcc tcgatcagaa aggtcggaac agggtcatcc
       61 tgcaggtcct tgccggtctg cttctgccag acgccacgcc accgaccagg ctcggcagcc
      121 aacagcgcct ccacggcagc ctcactgaag tcggaccggt tgaagagtcg ctgagcccat
      181 cgcggcaagg cctggcgctg cggctgcagg tcgcgttggt agcggaaggc tgtgcggacc
      241 agagccagct tgatgaaggg atcgtcgagg ttggtcgaca gtgcccagtg gctcaggaag
      301 atgatctggc ggcgtgtttc tggcggttcg tccaggagct tcaggaactc ggcgcctcgc
      361 ctgacagtgt gtccggtggc acggatcggt gggccaccgt gcaggcatcg cgcctggaac
      421 accgaccagt ccttgggcca cttgtcgccg accgacgtcg gcaccatcac gacgatctga
      481 cgcgtcggat cctgctgcac gatcgacgca gcaacggcta acgcgacaaa ggtcttgccc
      541 atgccgacgt catcggccag cacgactccg ggctggctgt tcagacgccg gcaaatctcg
      601 gcggcggtat gcatctgccg ccatgcatcc cgctcgggaa cacggtcgct gcgcaggtcg
      661 atgtgcgggc tgagtgccca gggttgactc atgcaccgac ctcttggaag acgttctggg
      721 tataggtgtc aatcgccgga tgcctcgtcg tgttcaagcc gtcgacgatg cccttcagga
      781 cacccaaggt ctgggcaatg cgccggtgca cgacctgctc cggtaggtca acgatgaggg
      841 tgccccacgg gacgcggtgc acagtcagcg ccagctctcc gatcaagaac tgggcttcgc
      901 cagccaatgc accatcgcac gatgcggcgt ccgcgagctt gcgcgccaca tgctccggcc
      961 cgagcgtccc agccagtcgc cattccagcg cgtcgaggct tgagcaccgg gccgacagtc
     1021 gacgcgcgat gccccacaac gccgccgagg cgcgacgcac gcgctgaatc agcagaccag
     1081 agtcgtcgaa gcgcttgagt ggatccagtt cgtcgccacc gggcttcttg cgggtcgact
     1141 tggcccgcag ggcgttttcg atcgcctcgc gcagcggcct ggtcgatgcc aggatggcca
     1201 gcaacacatc ggacgacagg ccggtcagct cggaaggtgg cggcaacacg gcctctggac
     1261 cgatgttgac aatccagccg gcacgattcg attcacctgc tgcggtccag tcgacatcga
     1321 gcaagaaggg cagcacctcc aggttcgccg gcaagctgac ctccatgcgc acgggttgcc
     1381 cgtgaccccg ccaggccgtg gcatccagca agtggacccg cacctgacct ggtggcgtcc
     1441 aaccgagtgt ccagctctct ggcaaccgat cggtcctgaa gctcagctcg atgcattctc
     1501 ggcgcatcaa cagtgcttcc tcgaagccat cgggcaagtc ggcaacagcc gattcatctt
     1561 cgtcatcgct ggcgggctca aattccagac cgccgtcgag ttcttcgcca acggggatca
     1621 gcgtcgccag tttctttgcg tgcgggctgt cgactcggca accgagccac aggttcagtt
     1681 cgcggtgcgg cgcccggtcc agaccgagcc ccttggcggt gatgttcgac gaaccgaaca
     1741 tcaccgcgaa ccaggtcgcg gattggtact ggatgcactt ggcgtgcaac cggcggtcgt
     1801 cctgcgctgc gaagctggcg accctcgctt ggatccgtga cccggctacg ctgcgcaact
     1861 gcacaggggc acgaacgatg gtgccaacgg cggtggtatc cactggcacg acgaaggttg
     1921 cacgcgtgcg agcacccgcc ggcgcgcgct tcgccaggcg ttcgagcact tctttcgcac
     1981 cagccatgtc tggcgtgtcg tcccagaacg gcgacatcgc gacgacggtc tgtggaggcg
     2041 aaccgctcca cacgtcggca agccgatcca gcgggcggct gtcacggctg ccagccgcca
     2101 gtgcgaactt cagcccctgg ctggcactcg gcttgaagct cagtgacgac aggcgggatt
     2161 cgaagcgttg aagaatgcgt tcggcgcgct gcttcggacc ggcccggtcg agcgcgccag
     2221 gcgccagggc gaggatgccg cgcagctccc gcaacaaatc ctcgaacact gccttcggca
     2281 cggcacagtc gtcatcgacg tcgattgcca tgcccagctc gacctggtgc cgatagccgg
     2341 cccgcgtcag gttggccgac ccgatgacca cgcgcattga gcgctcccag atcaacacca
     2401 ccaccttggc atgcagcagc ccgtgaggca cccgcaccgg cactaggtcc cagcgcagat
     2461 tgcgggcatc cggccggcaa ctgcgatcca cgagcacgca gacttgcgtt tcggccaggc
     2521 gctcttcctc ttcgagcatt ccggcgatat cgagaccggt ggcgccatcg ggatcgcggg
     2581 ccgagatcga caggaatcgg ctgaggcagt cctccgcgaa gaagtcggca tcgaaggtga
     2641 acgtcgtggc caggcaggcc agaggcaggc cagcaccgtc tttggacagc cagttgtcga
     2701 gcatcttcat ggtgccaagt ccttcatgaa gccacgcagg gccgaaagcc gataggggtg
     2761 caccagcgtg cctgggacga gctgcgctgc ctccgaccgc cagtagattg aacgcacgac
     2821 ccatccaccg gcgtggcggt cgagccaggg ccgtttgccc ttgggcggtt tctggttctg
     2881 cacggcttca tgatgcgtca tcaacaaatc gacaaggtcc gaggtcgagc cgggttgctc
     2941 aaacttgcca aggcgatggc tgaacaccac cagcagttcg ccgtcgatgc gctccagaca
     3001 atcggccgca cgcttcatgg catcgggcaa tcgggcacgg gcatcgttga ggacctgatc
     3061 gcgcgacaca tcggccgcag tgagtggacg agtgcccagc atggtggaca agtggcagac
     3121 ctgcgcgaag gatgcaccca gcagatgtgc cacggcttcg taagcggaga tggcttcgag
     3181 gcgtaaccgc aggtccaatg atcgctggcc cgccagaagg gacttcagca catcggcttc
     3241 gctggcatcg ccctgctggt cgagaaagct tgccaactcg gcgcgcatcg gcatgttgtc
     3301 gtccacgatc cagtggcgaa gcaacgcctt ctctgccctg ccggcctgtg tcgggtgcaa
     3361 ggcttcgctg agctggcgcc acaggtggga accctcgggc gcggcgcaat gaccgtgctg
     3421 cagggccgca cggacttcat cacgcaggcg cagtctcaac cgcccgccat ccgtgcccgg
     3481 cttgccgtcg gtgtagccgc tgaggccacg gtcccgctcc caggcttggg tcaagtcgct
     3541 gagtctggcg gccacgcggc ggtcctcgtc cagcacttgc aggtgacgtg ccagcggcag
     3601 gtagatgccg gtgaagccga acacgttggg cgacttcagg tagttgcgtg cggccaaccg
     3661 ctttccctgt gccagaacac tgcgcacctt ggacgatccg ggaatgccgg atgcctcgag
     3721 cggcaggtcc ttgccgctgc gccgcgcaaa ggcttcgagc accagccatt caaagcagat
     3781 cgttggcgtc gatatgcggt ccaccggcag cacgtcgacc aggctctctg cagccagcgc
     3841 gccaactgct gaagcggtca ggaatcggat gcggcgcatg cgcgctcgca gacccggcag
     3901 aagcgactcc gcgagtcggt ccgcgagttg ggccatgccg agcgggtcca gactgccttc
     3961 gccgctgatt gtcggatcgg cgtccgaaag ttgaggcagt gtgagattca tcgttggcac
     4021 ttggaagtca atccatcaca gcatgcgagc agtggggctc ggccacgtcg agcttccgtc
     4081 catcgaccgg agtgccacag gaaatcaatc agttccttca cggcctcggg attcactctt
     4141 cgccatgacc gagtaagcca ccagtgcgct tgacccgacg ctccacagcc aagtcaagca
     4201 cgcctcggcc gccatccgac gttgccatgg tgaaccagtg ccgggctcgg gtgatgccgg
     4261 tgtagatgag ttcgcgcgtc agcacgcggc tcggcgtttc cggcagcacc agcgcggcgt
     4321 gggtgaattc cgatccctgg gacttgtgca ccgtcatcgc gtacacggtc tccaccgcct
     4381 gcagccggct gggaagcacc cagtggatgc tgttgctgcc atcacccgcc gcaaaggcca
     4441 cgcgcaggat ggcgcctgtc gcggcggggt cggcgccacg gcgctgcggc acttgcagcg
     4501 tgatgccgac gtcgccgttc atcaggccca gcgcgtagtc attgcgcgtc accatcactg
     4561 gccggccctc gtaccagccc tgggcggcgc ttgtcagccc ttcgacgcac agcaggtcgg
     4621 cgatgcgctg attgagcccc tccacgccat gggcgccttg acgcactgca cacagcacct
     4681 ggaactggcc gtaggcgacc agcacctcgc gagcccaggc gtcccaggct tctgcgtccg
     4741 catcggccgg cggacggcgc gcccgtagcc gctccaggta gtgccggtag cccaccggcg
     4801 gcgccacggg ctcgccgcgg tcgatgcgcc cctggccttg ccccgcaaac gcgccgcagg
     4861 cgccatccag ggccagacgc gccagggcct ggctgtcagc cgccaagttc agccaggcca
     4921 gatcgggtgg caccacgtcc cgcaccgtgc gcaggcctga catggacccg gcattgacgg
     4981 catcggccag ccgcccgatg ccgctggtag cgtcaaaacg atggctgaag cgcagcttca
     5041 cgatggtctg gtccagcggc aagccctggt cgtcgttcaa ggccagctca accgtctcgt
     5101 tagccacctg ctgcacccaa gcccgtgtgg cagcggtgta gtggcctttg tcggcacgcc
     5161 ggcacaggtc gcccagcacg gcgccggcct ccaccgacgc gagctggtcc ttgtcgccca
     5221 gcagcaccag ccgggcctgc gccggcaagg ccgcaacgac cgacgccatc atttccagat
     5281 cgaccatcga ggcctcgtcg atcaccagca cgtccaccgg caaggggttg cgcacgtcgt
     5341 ggcggaagtg ccgtgtgtcg ggccggctgc cgagcagccg gtgcacggtg gtcacctcgg
     5401 tggggatggc cacgcgcagc gcctcggcgt ccggcagccc gtccagcggt agccgctgca
     5461 ccgcgccggc gatcgactcg ttcaaccgcg ccgcggcctt gccggtaggc gcggccatgc
     5521 ggatgcgcag gcggcgagcg gtggggctcg cgttcatcgc caggtgctgc agcaccgcca
     5581 gcaacttaac aacagtggtc gtcttgccag tgccggggcc gccggtgatg actgcaaacc
     5641 catgccgtgc ggccagcgcg caagccacct tctgccagtc ggtgccctgt gccgccggca
     5701 cctgcgggaa cagcacctgc agcgcctggc gcaaggccgg ctcgtcaggc gggatgacct
     5761 gatcgaggcg ctgcttgatc gcctcgcgca ccgtctgctc gtactgccag tagcggcgca
     5821 ggtacaggcg cgcgccgcac agcaccagcg gcgtgttgcc gctgccgtcg cccaccaggc
     5881 gcggatggtg caaggcctgc tgccaggtgg ccaggtccac gccggccagc agcgcagccg
     5941 gcgaccgggg cgtggcggcc gcgcccacgt ttgcggcagc tggtggctcc ggcggcagtg
     6001 acagggcgtc ccgaacatcg gccaacgtcg cccccaggtc gaggcaggca tggccgcgac
     6061 ccagttggtg gctggccagt gcggcggcca gcacgagcag cgggttggcc ggnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgggcag ccgcgagcgc agcagccggt
     6241 gcagcgcgaa caggtacagc gtgtactgca actcatagcg gtggtgcagc actgcatcgc
     6301 gcatggcctg cggtgtgtag tgcgcgtcgt ccggccccag ccaattggac ttgtagtcgg
     6361 ccacgaagta gcggccttcg tgctcaaaca ccaggtcgat gaagcccttg agcatgccgt
     6421 tgagctggtt gggctgcagg ggcacgcgcg gcgcgccgtc caacgtgtgg cggctcacca
     6481 gcgcatcgag cgcccgggtg ttcacctgtg cggccggaaa ccagaactcc atctcgacct
     6541 ggatcgcgcc aagctcggtg gggcacactg gtgaggcgcc cggcagatgc tgctgcaacg
     6601 gccagggcgt gcgcagccag tggcccagcc agtcgcgcag cggctgcgtc cagcggctga
     6661 atcctcccag gttgcagcgg cgcgcgatca ggtccgccac ttcgcccggt tcatcgcgca
     6721 cacgcgcaaa gccctgtcgg ccggcccatt ccagcaggcc gtgcagaaag ctgccggccc
     6781 cggcaccgcg cgggaacgga tgcagcccgc ccgcgaccgt gtgggcgcca gcgtctggcg
     6841 ccgcggaggc caccggcgcc gtggccaggg tcagcggggc atcggcctgg ctctcggcat
     6901 agatgtcctc ggcggcgctg cgcgcgccgc tgtctggcaa ggtcaccggc atcacgcctg
     6961 ccgcactcgc gtcatcacca gcagttaggt cgacggcgcc gccggactcg ccacgaccag
     7021 gcccggtggc cagcagcgcg gagtagctgg cgatccacca gggctcgggc ggtgcggccg
     7081 gcagcggcgg ctcgggccgc ggctgcgctg acatggcctc aggctgccag gtgtcggggg
     7141 cgaccgtcgg tgcggtcagc accgcgatcg cctggcagtc gccgcgcagg gcttgcaggt
     7201 gcgccgcgag ctgaccagcc tcgatcgcct cgccacccga cagcagcgag cccagggcgc
     7261 cgcgttcgag ctgcttgagc ggcgccacac ccacccaggt ggcaaaacgc gcgcgggtca
     7321 gcgccacgta cagcttgcgc aggtcctccc ccaggcgctc gcggtccacg caggccagca
     7381 cggccgcgtc ggccttcagg tccaccctcg gccggccctg gtcgtcgtgc cacttcagcg
     7441 gcaggtcggt gtccttggcg gcgcggtagg cgctggcaaa gggcagaaac accagcgggt
     7501 actccagccc cttggacttg tgcaccgtga ccaccttcac caggtcggca tcgctttcca
     7561 gccgcaactt gcggatgtcg ctgtcgccac cctcgtcggc gcgctgctcg gccagatggc
     7621 ggatcagcgc atgctcgccg tcgagctgcc cgctggcctg ctgcatcagc tcggccaggt
     7681 gcagcacatc ggtcagcgcg cgttcgtcgt gccgggccag cagcccgcgc ggcacctcga
     7741 agtcgtgcaa caggcggcgc agcatcggca gcacgccctg gcgccgccac acctggcggt
     7801 aaccacggaa ctgcagcacc cgctcctccc aatgcagctc gtcgtggttc aaccggtcca
     7861 gttcggccca cgacagcccc agtgtggcgg tgccgatcgc cgcgcgcagc aaccggtcgt
     7921 cgtcgggctc ggcgcaggcg gcaagccagt gctgcaagtc gccggccacg gcgctggcgt
     7981 acaccgactc cttgtccgac aggtagacgc tgcgaacctc gcgctcaccc aaggcccggc
     8041 gcagcgcctg cgcctcgcgg ccggtgttca ccagcacggc gatgtcgccc ggctgcaccg
     8101 gccgcatcgt cccgtcgccc gcccaaaagc ccgcccgccg ctgctggccg gcccgcagca
     8161 gccgcaccac ctcggtggcg cagatcgccg ccatctgctg ctgataggcg gtggccgaga
     8221 tcgggtcgcc cgcgtcgagc tgccagaagc tcagcgccgg cgccttggct ccgtcgatgc
     8281 accagcgctc gtcacgcccg cgcgcagcga cctcgatgaa cggcaccggg tcgtcatcgc
     8341 cgttgcggaa gaggaaggcg cccggccccg ggcgctgctc ggcctgcctg aacacctggt
     8401 tgaccgcggc caccatcgcc ccggtggagc ggaagttggt gcccagcgtg ctgtggcggc
     8461 ctgcagtgtc gcgccgcgct gccaggtagg tgtggatgtc ggcgccgcgg aaggcgtaga
     8521 tcgcctgctt ggggtcgccg atcagcacca gcgcggtctg cgggtcgttg cccgccacgc
     8581 cgtagatcgc gttgaagatg cggtactgca gcggatcggt gtcctggaac tcgtcgatca
     8641 gcgccaccgg gaactgttgg cggatcagcc cggccaggcg cgcgccgttg gggccctgca
     8701 gcgcggcgtc cagcctcgac agcagatcgt ggaaacccat ctgggcccgg cgctgcttct
     8761 cgtcggccag gcgcgcggcg atccagcacg cggcgtggcg caacagctcc gtgcggccgt
     8821 ccggcagggc ctggatgcgc tcgcgcagcg tcagcatgtc aagcagcgcg ggatggtcgg
     8881 gcggcgcacc cttggcccag gcatcggtca ggccctcagg cgtcaggcgg gtccatccgg
     8941 agctgccatc aggcagcggc agcaccgcag caccgtcgac ccactcctgc agtgcctcca
     9001 gccaaggctg gtaatagcgc gcctggagct tgcggccgtt cacctgtttg gaggccacgg
     9061 cgcggtcgag cattgaccgc agttcaccca cccactgctt ccaaggcgcc ttgatgattg
     9121 ccaactcggc acccgcctcg ttgcgcacgg catccagcgc tagcgcgggc ggctcggcgt
     9181 caggcagcgc atcgcccagg tccagcagct cgcccaccaa ttggccaaga tcgtccggtg
     9241 acttccacca ggtgcgcagc tgggccacat cgacggcggc caacgggtag tagaagctgc
     9301 gccagtagtc gcgcacggcc tcggccaaca ggtcgcgctc gtcggtctgc aactcctgcg
     9361 tgaacaggct gccgctgtcg aaggcgtgct cgcgcagcat gcggttgcac cagccgtgga
     9421 tcgtcgacac ggccgattcg tccatcgcct cggcggccag ccgcagcgtg cgcgcacagc
     9481 ccggccacaa cgctggggca tgctcggcgc gcaggtcgtg cagcagatcc tcaccgcgcg
     9541 ggcgatcggg cacctcggcc ggatccagcg caaacgcctc ggcggcctcg gccagccggg
     9601 cgcggatgcg gtcgcgcagt tcctgggtgg cggcgtctgt gaaggtcacc accaggacct
     9661 cgggcggagt cagtggccgc gtgaacgcgg cagtgccgcc atgaccgagc accaggcgca
     9721 ggtacagcgc agcgatcgtg aaggtcttgc cggtgccggc actggcctcg atcaggctgc
     9781 tgccgcgcag gcacaggcgc aacggttgca gcagcggagg cagggtcgtc gtcattcggc
     9841 atctccggtg gccagggcct tcgaggcatc gcccttggct tcccttttct tgtggggcat
     9901 cgcgtccatc agtggccgca gcagcgcgtc ggcccaggtg gcgaacacgc cgcctgccca
     9961 cagcgcctga aagtcgggga acgccctggc caggtacgcg tcgccgtcgc gctcgccgaa
    10021 ctggttgaat ttgggctcgt ggtcctcgta cttgacgcgg gccgcctcaa aggcatcggc
    10081 ggcgtgcggc gacatcccgg catggccacc cttgcccagc cagacgaagc cgcagcccag
    10141 cgccagcggc agcgggctgc ggtggcccac ttgccaggcc tccaacagcg cctgccagcg
    10201 ctggcgcgcc tgcagggtat ccatcgggcc gaagctcacc tgtcctgcct tgctgacgac
    10261 caccgtggtc agcggttcgc ccgccagatg tccggccagg tgcgccaccc agaacggcag
    10321 cagcttgtcg tggcggtact tgttctcctt gaccaggcta ccgctgtcca gcagtacccg
    10381 accacgctcg ccgccggtgc tgcggcgcat gccgcctagc cagtccacca gccgctgtgc
    10441 ctccggaaag ccttcgggca agtgatccag cggctcgtcg gggcagccct ggggccagtc
    10501 cgccagggcc tgcgcatagg cgtcgaacat cgcctgcatc ggctcggcca gttcgccttg
    10561 caccagcttg ccgaactcgc cggccggcag ctcgccgcgc agggcgatgc ggtcgagttg
    10621 cgcgtccagc gccgcctggc gcgagccacc cgctactagc gcggccttct gcacctggat
    10681 cagctcgtcc tgcagttgcc aattctcgag cgcgtcgatg gcaaacggct cctggtcggc
    10741 gcccactggg tcgtcggtct cgaaacggat gcccaggcgt cgctggaaga agctgcgaac
    10801 cgggttcttg acgaagtcgc tcaccagctt gagcgtcagc ggcccgggtt ccccgccgtc
    10861 cggcaagtgc gcggcggcgg tcgctagccc gacaccatcg gcctgggttc gcccggcctc
    10921 aacggcccgc cactcgcggg catagctgaa cagccgctcg tcgcctccct ggttgaagta
    10981 cgccggattg aagggctgca ggcggtgcgc caccgtcagc gccgtcagca gggccgcccc
    11041 ggccttggcc tgcggctggt cgtcacctgc ctgccgccag cctgcggcca ggtggtcgcg
    11101 caactgcgac accagcactg agggcgggcg ctcctggttg tcgcggatgc tgcggcccac
    11161 ccagctgagg tgcagccgct cgcgcgcaga cagcaacgct tccaggaaca ggtagcggtc
    11221 atcctcgcgg cgcgagcggt cacccggccg cc
//
LOCUS       DAOORV010000115        11196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7280_length_11196_cov_12.262634, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000115 DAOORV010000000
VERSION     DAOORV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7280_length_11196_cov_12.262634"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKV56_13675"
     CDS             complement(<1..670)
                     /locus_tag="PKV56_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOX68908.1"
                     /translation="MRTTGTSVTTTTLGEAVQAFVPHALPPADPSLAAESYATANHRA
                     EMALARLGGVAGLVPSVDWLLYSAIRKEALLTSQIEGTQATLTDLFDDEAGQVLTNTA
                     DVEEVTNYLRAFRLVRDNLRSEAGLPISVRLLCDAHRLLLDGARGAGKQPGELRRSQN
                     WIGGARPGRAAFVPPPPDKVPGLLADLERFIHDPAPALPPLVRVALVHVQFETIHPFL
                     DGNGR"
     gene            complement(1078..1803)
                     /locus_tag="PKV56_13680"
     CDS             complement(1078..1803)
                     /locus_tag="PKV56_13680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOX68909.1"
                     /translation="MQAEAAPIVRKVKDIEYRLLPGTARQTTDIVIERDGCVTVRPPA
                     RMTPEQVDETVLSRRHWIYRNLAEWRDLNATRVVREWVNGESFLYLGSAYRLLLVPEQ
                     DEPLKLKDGRFCLLRSVVEQDGKTAAQDAFRDFYIARGMSRLVQRVNHFAPRVGVQPG
                     PLQVKELGFRWASSLPGGGLHFHWKCLMAPPKITDYIVVHELCHMHHRDHTDLFWNEV
                     DKVMPDYRERKEWLRQRGAGMDL"
     gene            complement(1793..5065)
                     /locus_tag="PKV56_13685"
     CDS             complement(1793..5065)
                     /locus_tag="PKV56_13685"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOX68910.1"
                     /translation="MSEYSEVEQPFLQQLAAQGWTVVDQGPQLPQDAAPSLRPSFRPW
                     WLPGVFREAVRGLNTLPDGRGEGAPWLTDHQLDELESQLFRQPQRTLLEANQAVHELL
                     LKAQVDVNEATGEVDPVVKLIDFHHPERNRFHAINQFRIATPGCVREFIVPDIVLFVN
                     GIPLAVVECKKGSATCANPMQEAFVQLQRYMRKRAETEAAGLKEGEPRLFHSNLLLVR
                     TCGLEADYGSISSGEEHFYAWKTLYPADDAARAGMNPQQQLVAGMLMPANLLQMLRTC
                     TVFMDTDAGARVKVVCRYQQFRAAGKIVQRLRAGHTAAERSGVVWHTQGSGKSLTMVF
                     VARMLRASQDLSDFKIVLVNDRQDLEEQLGETATLIGGRVNVIGSTQDLRRHLATDSS
                     DINMVMVHKFQEREQVLSNAVAEALGTYRAMPAGKTFGVVNASDRIVLMIDEAHRTQG
                     SDLGENLFEAFPNATRLAFTGTPLITERHGERKTHKRFGEYIDTYKLLDAVNDGATLQ
                     ILYEGRTADSALNEKHAFDEKFEDLFRDRSEEELAAIRKKYGATGDILEAKTRIDAIA
                     RDLVRHYVDNILPNGFKAQVVCHSKLACVRYQEGIDAAIAERLAAEQAKARPDAELLR
                     RLSFLKTAVVVSSDGTNEAAYITHARKQAARLNAVENFCKGFDLDDPDKANTGIAFLI
                     VCDMLLTGFDAPIEQVMYIDKRLREHTLLQAIARTNRVKRGKQRGYIVDYIGLANHLT
                     EALTLYTASDELQELHDGLKSITTELPVLEERYQRLLQHFNARGVNGIEAFVTGKLGD
                     MRQAAAVVHAAVKALADERYRADFEVYLKKFLMSLDIILPHTSAQAFRVPAKRFGYIL
                     QVAKERYKDESLQLGDAGEKVKALINEHLVSLGINPKVPPVELLAEDFLHRLQQHAGG
                     NDEAKASEMEHAIRKHCTVHHDEDPAFYKKLSEKVDALIERHHDEWDLLVEKLEELRT
                     EAVAGRKQGVEGLGTEASTFYEHLADVAYGGTTVPAADLPAFKVLMATLVELLQDTIG
                     SIDFWQSADKQKRLRGEIKTAIARTGLEAVLAQRERVAVEVMKLAKNRHDQLLKAAND
                     AS"
     gene            complement(5090..6259)
                     /locus_tag="PKV56_13690"
     CDS             complement(5090..6259)
                     /locus_tag="PKV56_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOX68911.1"
                     /translation="MIHYTQGNLLESKAQALVNTVNTVGIMGKGIALMFKDRFAENFR
                     LYAQACKAGDVRTGQMFVTEPGELDGPRWVINFPTKRDWRAKSRLEWIVEGLQDLRRF
                     LVENHVRSVAIPPLGAGNGGLDWPLVKDQIERALGDLDIAITVFEPTRTYQNVAKRHG
                     VEALTPARAMIAELVRRYWVMGFECSLLEVQKLAWFLNRTLDRDVPGNNPLKLRFEAN
                     KFGPYAHGLTKVLDQLDGSYLGSEKRIGDAKPFDVVWFNEQRTQVVQAYLRSEAKPYL
                     AALEDTSALIDGFESPYGMELLATVDWLLARGNVEPTVQAVRDGLARWPHEDGAERKT
                     RLFDDRAVGIALNRLLQSPRTGERPGLLQTIAAWEPLQDGLPPREDLAPQERDPL"
     gene            complement(6259..6912)
                     /locus_tag="PKV56_13695"
     CDS             complement(6259..6912)
                     /locus_tag="PKV56_13695"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOX68912.1"
                     /translation="MADDYAKLLNPERALIFRIIHRANLPWVLQHGLHCGNSATRAAD
                     WVSIGNQELIGKRAHWPVTVGPGGVLNDYVPFYFTPFSVMMHNINTGWNGVPRHPKPD
                     ILILVASLRRVVELGLTVLFTDSHANNPLVNYYRDPAQLDRIDWPILQARDFKRDPED
                     PDKFSRYQAEALVHRHCPLQGLSGIVCYNQGVKIQVEQAVAAAQVPLQVAARPAWYF"
     gene            complement(6925..8229)
                     /locus_tag="PKV56_13700"
     CDS             complement(6925..8229)
                     /locus_tag="PKV56_13700"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOX68913.1"
                     /translation="MPDWRTVQLHEVADIRVSNVDKKAVAGELPVKLCNYMDVYGNDY
                     IRADLSFMEATATAAEIERFGVRRGDVMITKDSETPDDIGIPSVVLDDIESLVCGYHL
                     AQLRPREAVVDPIYLSKQLSLPETSAYFAQRATGSTRYGLSTRTIAQTPIRLAPLVEQ
                     QRIAAILTSLDTAIAATEALIEKHHQIKAGLMHDLLTRGVADGGSLRRSALEAPDLYV
                     PTKIGLVPVGWEVTGLQAKQRGADKWIRTGPFGSSLKGEHWVVEGCPVVTIGSLGEGL
                     FDEHELLFVGERDAARLAEFRLKLGDVVFSRVADVGRSVAVRQENVGWIMSSNLMRIA
                     VDASQVRPDFLQMLLAGDARVKAQIRAKVNSGGREVANSEILGQLLFAWPPVDEQERI
                     IERVQSIEAPIRLDQDRLRKLRMQKQGLMHDLLTGKVPVGAG"
     gene            complement(8222..10765)
                     /locus_tag="PKV56_13705"
     CDS             complement(8222..10765)
                     /locus_tag="PKV56_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX68914.1"
                     /translation="MPHQRLTLARLEQLLLTACDDLRGNMDASEYKEYVFGMLFLKRA
                     SDLFEQRQAQIRAEGERDGLAPAAIEANLEDPDQYAGQYFYVPARARWNTPWTEPETG
                     TRHPALKHIKENVGSMLNKALEALEDANADALQDVLKGTINFNKKIGSTTLDDDTLVN
                     FIQNFDKIPLKDEDFEFPDLLGAAYEYLLKDFADQAGKKAGEFYTPAEVVRVCVEVCD
                     PQEGMRIYDPTCGSGGMLIQVRDYLREHGRNAANASLFGQEKMGTTWSICKMNMLLHG
                     IPDADIRQQDTLREPQHQDEDGQLKRFDRVLANPPFSQNYIKKDIKFPGRFPVWLPEK
                     GKKADLMFVQHMLAVLKADGKLATVMPHGVLFRGGEEREVRRQFIERRVLEAVIGLPA
                     NLFYGTGIPACILVLNKAGAATREHALFINADREYREGKAQNHLRPEDIDKIVHVYRA
                     GQDVSAYARRVPYAEIAAEDYNCNIRRYVDNAPPPEPHDVRAHLHGGVPVAEIDALAH
                     FWSNYPGLRESCFLPRGGADAERYADFSPALADKRAIATNVAQHNGVQQRHAEFIAQL
                     QAWWQQHLPVIDALAPDPTNQQGAPRNVYAVRATLLDSIERVFGGQHLLTRYQVRGAF
                     ANFYKQLSADFKSIAASGWGAELIPDQDILQSQFPQVLAELEQQHARLAELQALFAAA
                     GEEDFEDSDGTGVLPPEQVKDLKAQLKEAKGQAKLAKKDSSFGDWKSHQAEADRLDAL
                     LARHKALEEEARGLKARIKSTEKNRDELVEQARQRIGRDEARQVILQRLQAVLMDTYG
                     QYLRADQRACVAAIENLWGKYAVTAAHLEQARAGATAELTHFLSGLGYA"
BASE COUNT         1754 a         3719 c         3603 g         2120 t
ORIGIN      
        1 tgcgcccgtt gccgtcgagg aacgggtgga tggtctcgaa ctgcacgtgc accagggcca
       61 cgcgcacgag cggcggcagc gctggcgccg ggtcatggat gaaacgctcc aggtcggcca
      121 gcagccccgg caccttgtct ggtggcggcg gcacgaacgc ggcccggccg ggccgcgcgc
      181 cgccaatcca gttctgcgag cgccgtaact ctcccggctg cttgccggcg ccgcgtgcgc
      241 catcgagcag cagccggtgg gcgtcgcaca gcagccgcac cgagatcggc aggcccgcct
      301 cgctgcgcag gttgtcgcgc acgagccgga aggcgcgcag gtagttggtg acctcttcga
      361 catcggccgt gttcgtcagc acctggccgg cctcgtcatc gaacaggtcg gtcagcgtag
      421 cctgggtgcc ctcgatctgc gaggtgagca gtgcctcctt gcggatggcg ctgtagagca
      481 gccagtcgac cgagggcacc aggccggcca cgccgcccaa ccgggcaagg gccatctcgg
      541 cgcggtggtt ggcggtggcg tagctctcag ccgccagcga cgggtcggcc ggcggcagcg
      601 catgaggcac gaacgcttgc accgcctcgc ccagcgtggt ggtggtgacg gaggttccgg
      661 tggtgcgcat cgtatgaaag ctggcttgag cagtgcgcct tcatagtaaa gtattcttta
      721 attcatggaa agcctgcgaa agcacgcttg caggggcccg cagcgccaac gcccatacac
      781 tctcgcgcca agtggatcgg gggttcctgg ttggcgctac agcctcggtg ggttcactgg
      841 ggcttctcgc tttctggccg gcaagcgcca agagtactta gcaagtgcgc ttaacaagct
      901 aagcgctaag cctttgattt gaaagaggtt tcggaaacgg gccggcgcca accccaatcg
      961 ctaagcgcgc accatctccg cgtcgcggat cggactcagg ccgcggatgc gggtgaaggc
     1021 cgccttgccc tccagttact tgaccacaga ccctcgtacg cggaccactt cgcccaccta
     1081 cagatccatc cccgcccccc gctgcctcaa ccactccttc cgctcacgat agtccggcat
     1141 caccttgtcc acctcgttcc agaacaggtc ggtgtgatcc cggtggtgca tgtggcacag
     1201 ctcgtggacg acgatgtagt cggtgatctt gggcggcgcc atcaggcact tccaatggaa
     1261 gtgcaggccg ccgccgggca ggctgctggc ccagcggaag cccagctcct ttacctgcag
     1321 cggcccgggc tgaacaccca cacgtggcgc gaagtggttg acgcgctgga ccaggcgcga
     1381 catgcctcgg gcaatgtaga agtcgcggaa tgcgtcctgc gctgccgtct tgccgtcctg
     1441 ctccaccacg ctgcgcagca ggcagaaacg gccgtccttc agcttcaggg gctcgtcctg
     1501 ctccggcacc agcagcaggc ggtaggcgct gcccaggtac aggaagctct cgccgttcac
     1561 ccattcgcgc accacgcggg tggcgttcag gtcgcgccat tcggccaggt tgcggtagat
     1621 ccagtgccgg cggctcagca cggtctcgtc cacctgctcg ggcgtcatgc gcgcgggcgg
     1681 gcgcacggtg acgcagccgt cgcgctcgat cacgatgtcg gtggtctgcc gcgccgtgcc
     1741 gggcagcagg cggtactcga tgtccttcac cttgcggacg atgggcgcgg cctcagcttg
     1801 catcgttcgc ggccttcaac agctggtcat ggcggttctt cgccagcttc atcacctcca
     1861 cggccacacg ctcacgctgc gccagcacag cctccaggcc cgtgcgggcg atggcagtct
     1921 tgatctcgcc gcgcaggcgc ttctgcttgt cagcgctttg ccagaagtcg atgctgccga
     1981 tggtgtcctg caacagttcc accagcgtgg ccatcagcac cttgaacgcc ggcaggtcgg
     2041 ccgccggcac ggtggtgccg ccataggcca cgtcggccag gtgctcgtag aacgtgctgg
     2101 cctcggtgcc caggccttcc acaccctgct tgcggccggc cacggcttcg gtgcgcagtt
     2161 cttccagctt ctccaccaac aggtcccact cgtcatggtg acgctcgatc agcgcatcca
     2221 ccttctccga aagcttcttg tagaacgccg ggtcctcgtc gtggtgcacg gtgcagtgct
     2281 tgcggatggc atgctccatc tcgctggcct tggcctcgtc gttgccgccc gcgtgctgct
     2341 gcagccgatg caggaagtcc tcggccagca gttccaccgg cggcaccttg gggttgatgc
     2401 ccaggctgac cagatgttcg ttgatcagcg ccttcacctt ctcgcccgcg tcgcccagtt
     2461 gcaggctctc gtccttgtag cgctccttgg ccacctgcag gatgtagccg aagcgcttgg
     2521 ccggcacgcg gaaggcctgc gctgatgtgt gcggcaggat gatgtccagg ctcatcagga
     2581 acttcttgag gtacacctcg aagtcggccc ggtagcgctc gtcggccagc gccttcacgg
     2641 ccgcatgcac cacggcggcg gcctgccgca tgtcgcccag cttgccggtc acgaaggcct
     2701 cgatgccatt cacgccgcgg gcgttgaagt gctgcagcaa gcgctggtag cgctcttcca
     2761 gcaccggcag ttcggtggtg atgctcttca gcccgtcgtg cagttcctgc agctcgtcgc
     2821 tggcggtgta gagcgtcagt gcctcggtca ggtggttggc caggccgatg tagtccacga
     2881 tatagccgcg ctgcttgccg cgcttcacgc gattggtgcg ggcaatggcc tgcagcagcg
     2941 tgtgctcgcg cagccgcttg tcgatgtaca tcacttgctc gatcggcgca tcgaagccgg
     3001 tcagcagcat gtcgcaaacg atcaggaagg cgatgccagt gttggccttg tcggggtcat
     3061 ccaggtcgaa ccccttgcag aagttctcca ccgcattcaa ccgcgcggcc tgcttgcggg
     3121 cgtgcgtgat gtaggccgcc tcattggtgc cgtcggacga caccaccacc gcggtcttca
     3181 ggaaggacag ccgacgcagc agttcagcat ccggtcgcgc cttcgcttgc tccgccgcca
     3241 ggcgctccgc gatggcggca tcgatgccct cctggtaacg cacgcaggcc agcttggagt
     3301 ggcacaccac ctgtgccttg aagccgttgg gcaggatgtt gtccacgtag tgccgcacca
     3361 ggtcgcgggc gatggcatcg atgcgcgtct tggcctccag gatgtcgccg gtggcgccgt
     3421 acttcttgcg gatggcggcc agctcttcct cgctgcggtc gcggaacagg tcctcgaact
     3481 tctcgtcgaa ggcgtgcttc tcgttcagcg cactgtcggc cgtgcggcct tcgtagagga
     3541 tctgcagcgt ggcaccgtcg ttcaccgcgt ccagcagctt gtaggtgtcg atgtactcgc
     3601 cgaagcgctt gtgcgtcttg cgctcaccat ggcgctcggt gatcagcggc gtgccggtga
     3661 aggccaggcg cgtagcgttg gggaaggctt cgaacaggtt ctcgcccagg tcggagccct
     3721 gggtacggtg cgcctcgtcg atcatcagta cgatgcggtc gctggcgttc accacgccaa
     3781 aggtcttgcc cgccggcatg gccctgtagg tgcctagcgc ctcggccact gcattgctga
     3841 gcacctgctc gcgctcctgg aacttgtgga ccatcaccat gttgatgtcc gagctgtcgg
     3901 tggccaggtg gcgccgcagg tcctgcgtgc tgccgatcac gttgacgcgc ccgccgatca
     3961 gcgtggccgt ctcgccgagc tgttcctcca ggtcctgcct gtcgttgacg agcacgatct
     4021 tgaagtcgct caggtcctgg ctggcgcgca gcatgcgggc cacgaacacc atggtgagcg
     4081 acttgcccga accctgcgtg tgccacacca cgccgctacg ctcggccgcc gtgtggccgg
     4141 cgcgcagccg ctgcacgatc ttgccggcgg cgcggaactg ctggtagcgg cacaccacct
     4201 tcacccgcgc gccggcatcg gtgtccatga acacggtgca ggtgcgcagc atctgcagca
     4261 agttggctgg catcagcata cccgccacca gttgctgctg cgggttcata cccgccctgg
     4321 cggcatcgtc agccgggtac agcgtcttcc aggcgtagaa gtgctcctcg ccggagctga
     4381 tgctgccgta gtccgcctcc agcccgcagg ttcgcaccag cagcaggttg ctgtggaaga
     4441 ggcggggttc gccctccttc agaccggcag cctccgtttc ggcgcgtttg cgcatgtagc
     4501 gctgcaactg cacaaacgct tcctgcatcg ggttggcgca ggtggccgaa cccttcttgc
     4561 actccaccac cgccagcggg atgccgttca cgaacagcac gatgtccggc acgatgaact
     4621 cgcgcacgca gccaggcgtg gcgatgcgga actggttgat ggcgtggaag cggttgcgct
     4681 cggggtggtg gaagtcgatg agcttgacca ccgggtccac ctcgccggtg gcctcgttca
     4741 cgtccacctg cgccttcagc agcagctcgt gcacggcctg gttggcctcc agcagcgtgc
     4801 gctggggttg gcggaagagc tgcgactcca gttcgtcgag ctggtggtcg gtgagccagg
     4861 gagcaccttc acctctgcca tctggcaggg tgtttagccc gcgcaccgct tcgcggaaca
     4921 cgccgggcag ccaccagggc cgaaagctgg ggcgcaggct aggcgccgcg tcctgcggca
     4981 gctgcgggcc ctggtcgaca acagtccacc cctgcgcagc caactgctgc aggaagggct
     5041 gttcgacctc ggaatactcg ctcatcgcgg tggccccggc aggccacgcc taaagcgggt
     5101 cacgctcctg cggcgccagg tcctcacgcg gcggcaggcc gtcctgcagt ggttcccatg
     5161 cggcaatcgt ctgaagcaat ccgggccgct cgcccgtgcg gggtgattgc agcaacctgt
     5221 tcagcgcaat gccgacggcg cggtcatcga acagccgagt cttgcgctcg gcgccatctt
     5281 cgtggggcca gcgcgccagg ccatcgcgca cggcctgaac cgttggctcg acgttgccgc
     5341 gtgccagcaa ccagtccacc gtggccagca actccatgcc gtagggcgac tcgaaaccat
     5401 cgatcagcgc cgaagtgtcc tccagagcgg ccaggtacgg cttggcctcg ctgcgcaggt
     5461 aggcctgaac cacctgcgtg cgctgttcgt tgaaccacac cacgtcgaag ggcttggcat
     5521 cgccgatgcg tttctcgctg cccaggtagc tgccatccag ttggtccagc accttggtca
     5581 gcccatgcgc atacgggccg aacttgttgg cctcgaagcg cagcttcagc ggattgttcc
     5641 cgggcacgtc ccggtccagc gtgcgattga ggaaccaggc cagcttctgc acttccagca
     5701 ggctgcactc gaagcccatc acccagtaac ggcgcaccag ttcggcgatc atggcccggg
     5761 ctggcgtcaa cgcttccacg ccatgacgct tggccacgtt ctgataggta cgagtcggct
     5821 cgaagaccgt gatcgcgatg tccaggtcgc ccagggcgcg ctcgatctgg tccttcacca
     5881 gcggccaatc caggccgcca ttgccggcac ccagcggcgg aatcgcgacg gagcgcacgt
     5941 ggttctcgac cagaaagcgc cgcaggtcct gcagcccttc gacaatccat tccagacggg
     6001 acttggcgcg ccagtctcgc ttcgtcggga agttgatgac ccaccgcggc ccgtccaact
     6061 cgcccggctc ggtcacgaac atctgccccg tgcgcacgtc gccggccttg caggcctgcg
     6121 catacagccg gaagttctcg gcgaaccggt ccttgaacat caaggcaatg cccttgccca
     6181 tgatgcccac ggtgttgacc gtgttcacca gggcctgggc cttggattcc agcagattgc
     6241 cttgcgtgta gtggatcatc agaagtacca ggcgggccgg gctgccacct gcagcggcac
     6301 ctgggccgcg gctacggcct gttcgacctg gattttgacg ccctggttgt aacagacaat
     6361 ccccgacaac ccctgcagcg ggcaatgccg atggaccagc gcctcggcct ggtagcggga
     6421 gaacttgtcc gggtcctccg ggtcacgctt gaagtcacgc gcctgcagga tgggccagtc
     6481 gatgcggtcg agttgtgccg ggtcccggta gtagttgacc agcgggttgt tggcatggct
     6541 gtcggtgaac agcaccgtca ggcccagttc caccacacga cgcaagctcg ccaccaggat
     6601 caggatgtcc ggcttgggat gcctgggcac gccgttccag ccggtgttga tgttgtgcat
     6661 catcaccgag aagggtgtga agtagaaggg cacgtagtcg ttcaagacgc cgcccggccc
     6721 cacagtcacc ggccagtggg cgcgcttgcc gatcaactcc tggttgccga tactcaccca
     6781 gtccgccgcg cgcgtggcgc tgttgccgca atgcaggccg tgctgcagca cccagggcag
     6841 gttcgcgcga tggatgatcc ggaagatcag cgcgcgctcg ggattgagca acttggcgta
     6901 gtcgtcggcc atgggcgtgg actttcagcc ggctccaaca gggaccttgc cagtcagcag
     6961 gtcgtgcatc aggccttgct tctgcatgcg aagcttccgc agccgatcct gatccaagcg
     7021 aatcggtgcc tcgatggatt gcacgcgctc gatgatgcgc tcttgctcat cgacgggcgg
     7081 ccacgcaaag agcagttgcc ccaggatctc gctattggcc acttcgcgtc cgccgctgtt
     7141 gaccttggcg cgaatctgag ctttgacccg ggcatcgcct gccagcagca tctgcaggaa
     7201 atcggggcga acctgactcg cgtccacggc aatacgcatg aggttggacg acatgatcca
     7261 tccgacgttc tcctggcgca cagcgacgga ccggccgaca tctgcgacgc gcgagaacac
     7321 gacgtctccg agcttgagcc tgaattcggc cagacgtgcc gcgtcacgct cgccgacgaa
     7381 caggagttca tgctcatcaa acaatccttc gcctagtgag ccaatcgtga caacagggca
     7441 gccctcaacc acccagtgct cacccttcaa tgacgacccg aaaggcccgg tccgaatcca
     7501 cttgtcggca ccgcgctgct tcgcttgaag accggtcact tcccaaccta ccggcaccag
     7561 gccgatcttg gttggcacgt agaggtcggg ggcctcaaga gcggatcgtc gaagcgaccc
     7621 tccgtccgca acgccgcgcg tcaacaagtc gtgcatcagc ccggccttga tctgatggtg
     7681 cttctcgatc agcgcttcgg tggctgcgat ggcggtgtcg aggctggtga ggatggcggc
     7741 gatgcgctgc tgctcaacca acggcgccag gcgaatgggc gtctgcgcga tcgtccgcgt
     7801 cgatagccca tagcgcgtgg aacccgtcgc acgctgcgcg aagtaggcgg aagtctccgg
     7861 cagagatagt tgcttcgaca agtagatggg atcgaccaca gcttctctgg gccgcaactg
     7921 agccaaatgg tagccacaca ccagactctc gatgtcatcc aacacgaccg aaggtattcc
     7981 gatgtcgtca ggcgtctccg aatccttggt gatcatcacg tcgccgcggc gcacaccgaa
     8041 gcgctcgatc tcggcagcag tagctgtcgc ctccatgaat gacaggtctg cccggatgta
     8101 gtcgttgccg tacacatcca tgtagttgca tagcttgacc ggcaactcgc ctgccaccgc
     8161 cttcttgtcg acgttgctca cccggatgtc ggccacctca tggagctgaa ctgttctcca
     8221 gtcaggcata tcccaacccc gacagaaagt gcgttagttc cgccgtcgct ccagcacgcg
     8281 cctgctccaa gtgcgccgct gtgacggcgt acttgcccca caggttctcg atcgccgcca
     8341 cgcaggcccg ctggtccgcc cgcaggtact gcccataggt gtccatcaac accgcctgca
     8401 agcgttgcag gatcacctgc cgcgcctcat cgcggccgat gcgctgccgc gcctgttcca
     8461 ccagctcatc gcggttcttc tcggtgctct tgatgcgcgc cttaaggccc ctcgcttctt
     8521 cctccagcgc cttgtgccgc gccagcagtg catccagtcg gtcggcttct gcctggtggg
     8581 acttccagtc gccgaagctg ctgtccttct tggccagctt ggcctgcccc ttggcttcct
     8641 tgagttgggc cttcaggtcc ttcacctgct cgggcggcag aacgccggtg ccgtcgctgt
     8701 cctcgaagtc ttcttcgccc gccgcagcaa acagcgcctg cagctcggcc aggcgcgcgt
     8761 gctgctgctc caactcggcc agcacctgcg ggaactggct ttgcaggatg tcctggtcgg
     8821 gaatcaactc cgcgccccaa ccgctggccg cgatggactt gaagtcggcc gacaactgct
     8881 tgtagaagtt ggcgaacgcg ccgcgcacct ggtagcgggt gagcaggtgc tggccgccga
     8941 acacccgctc gatgctgtcc agcaacgtgg cgcgcaccgc gtacacgttg cgtggtgcgc
     9001 cctgctggtt ggtggggtcc ggcgccagcg catcgatcac cggcaggtgc tgctgccacc
     9061 aggcttgaag ctgcgcaatg aattcggcat ggcgttgctg cacgccgttg tgctgggcta
     9121 cgttcgtggc gatggcacgc ttgtcggcga gcgcggggct gaagtcggca tagcgttcgg
     9181 catccgcccc gccgcgcggc aagaagcagc tttcccgcag gccggggtag ttgctccaga
     9241 agtgcgccag cgcgtcgatc tccgccaccg gcacgccgcc gtgcaggtgc gcgcgcacgt
     9301 cgtgcggctc gggcggcggc gcgttgtcca cgtagcggcg gatgttgcag ttgtagtcct
     9361 cggccgcgat ctcggcatag ggcacgcggc gggcgtaggc cgacacgtcc tgaccggccc
     9421 ggtagacgtg cacgatcttg tcgatgtctt cgggccgcag gtggttctgg gccttgcctt
     9481 cgcggtattc gcggtcggcg ttgatgaaca gggcgtgctc gcgggtggcg gcgccagcct
     9541 tgttcagcac caggatgcag gccgggatgc cggtgccgta gaacaggttg gcgggcaggc
     9601 cgatcacggc ttccagcacg cggcgctcga tgaactgccg gcgcacctcg cgctcttcgc
     9661 cgccgcggaa cagcacgccg tgcggcatca ccgtggccag cttgccgtcg gccttcagca
     9721 cggccagcat gtgctgcacg aacatcaggt cggccttctt gcccttctcg ggcagccaca
     9781 ccgggaagcg gccggggaac ttgatgtcct tcttgatgta gttctggctg aagggcgggt
     9841 tggccagcac gcggtcgaag cgcttcaact ggccgtcttc gtcctggtgc tgcggctcgc
     9901 gcagcgtgtc ctgctggcgg atgtcggcat cggggatgcc gtgcagcagc atgttcatct
     9961 tgcagatgga ccaggtagtg cccatctttt cctgcccgaa cagcgacgca ttggccgcgt
    10021 tgcggccgtg ctcgcgcagg tagtcgcgca cctggatcag catgccgccg ctgccgcagg
    10081 tggggtcata gatgcgcatg ccctcctgcg ggtcgcacac ctccacgcac acacgcacca
    10141 cctcggccgg ggtgtagaac tcgccggcct tcttgccggc ctggtcggca aagtccttca
    10201 gcaggtattc gtaggctgcg cccagcaggt cggggaactc gaagtcctcg tccttcagcg
    10261 ggatcttgtc gaagttctgg atgaagttga cgagcgtgtc gtcgtccagc gtggtggagc
    10321 cgatcttctt gttgaagttg atcgtgccct tcagcacgtc ctgcagcgcg tcggcgttgg
    10381 cgtcttccag cgcctccagc gccttgttga gcatggagcc gacgttctcc ttgatgtgct
    10441 tcagcgccgg gtggcgagtg ccggtctcgg gctcagtcca gggcgtgttc cagcgggcgc
    10501 gcgccggcac gtagaagtat tggccggcgt actggtcagg gtcttcgagg ttggcttcga
    10561 tggcagcggg cgcgaggccg tcgcgctcac cctcggcgcg gatctgggcc tgacgctgct
    10621 cgaacaagtc gctggcgcgc ttcaagaaca gcatgccgaa gacgtattcc ttgtactcgc
    10681 tggcgtccat gttgccgcgc aggtcgtcgc aggcggtgag cagcagttgc tccaggcggg
    10741 ccagggtgag gcgttggtgg ggcatgcggt cggcagggtc gggtcaaagg gctcggccgg
    10801 tgattctctc aggcgctcgg cgcatgttca tcggtcacag tgaagcgcgt tttcggcggc
    10861 ttgaggggct ggaaagtccg gccgttgcgg tagtttcccc cgttcgccgg gcgcctcgcg
    10921 gtgactccgc tgccgcgcgt gtcggaccta ccagcaaccc acgaacgcga gcctcgtccg
    10981 cgcccaacta gaggcatggc agaagaaggt caagcccgag gggaagaccg catcgtcagt
    11041 gccgcaaggt gtcttgtcgc acctaattgc aactctgccc ggatcaagtg gtcggttctt
    11101 gcccgatgaa catgcgccgc gtttgcggcg ctgttgacat cacacgcacg cttgcggtca
    11161 actctcgatc agccctgcgg tgcctgcagc aggacg
//
LOCUS       DAOORV010000116        10940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7498_length_10940_cov_7.629949, whole genome shotgun sequence.
ACCESSION   DAOORV010000116 DAOORV010000000
VERSION     DAOORV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_10940_cov_7.629949"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKV56_13710"
     CDS             <1..1415
                     /locus_tag="PKV56_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOX68915.1"
                     /translation="QALPLRIVGIMQNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQ
                     LFGPIRGGLAYAVIFVGAMLAATTGVVAASVISMGLISLPIMLRHGYDRRVASGVIAA
                     SGTLAQIIPPSLVLIVLADQLGQSVGALYQGALVPALTLTGLYVVYVAVISVVKPQWV
                     PAMPASARTLREDPQASTGRSQVALFWRVGCALVPPLALIFLVLGTIFLGVATPTEGG
                     AMGAVGALVMAIVRGRLDMSLLRQAMQSTARLSCFVLFILIGSTVFSLSFQAVDGPKW
                     VEHLLTALPGGVLGFLIVVNLLVFLLAFFLDFFELAFIVVPLLAPVATALGIDLVWFG
                     VLLAVNMQTSFMHPPFGFALFYLRSVAPDREYTDTVTGQRMAPVTIGQIYRGAIPFVL
                     IQLAMVALIMAFPGLVSRGHDVPAGIDADVELRRMQSDQNAAETANPVPVTADGNSAD
                     EDPMKALQQAVEADRKDSPK"
     gene            complement(1428..2510)
                     /locus_tag="PKV56_13715"
     CDS             complement(1428..2510)
                     /locus_tag="PKV56_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOX68916.1"
                     /translation="MQRRRFIHHSGLAGVLAAGAAPAVVHAQANLRWRLASSFPKSLD
                     TIFGAAELFAKKVGEMTAGKFQISVHPAGELLPPFGVVDGVQAATVEMAHTVPYYYFG
                     KDETFAIGAAIPFGLNSRQMTAWMYRGNGLKLMREFYAKYNIINFPGGNTGVQMGGWY
                     RKEVKTLADIQGLKIRIGGFAGRVIERMGAVAQNIPGGEVYQALEKGTIDAVEWVGPY
                     DDQKLGFNKVAPFYHYPGWWEGGPQIDFFINTQAYAALPADYKAIVEMAASHVHVDMQ
                     ANYDAKNPAALKALVGAGVKVLPFPKSVLDEAFKQSMALYEELGAKNPNWKKVYDDYA
                     AFRREQNLWFRFAEASFDRYMQGIKL"
     gene            complement(2569..3966)
                     /gene="pmbA"
                     /locus_tag="PKV56_13720"
     CDS             complement(2569..3966)
                     /gene="pmbA"
                     /locus_tag="PKV56_13720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HOX68917.1"
                     /translation="MPSTTSPSSSSTGFAYSRADFQQLVDDALKLAASLGATDAGAEV
                     SEGVGLSVSVRKGEIENVERNRDKSIGVSVYVGQRRGNASTSDFSPAALEQTVRAAHD
                     IARFTAEDPAAGLPDTADLAFGDVATLDLDLFHPWDIDAEGAADLARRCESAALSVDK
                     RITNSEGAGVSAQQSHFFAGNSRGFRGGYASSRHSLSVAPIASLPGRNGDDMQRDAWY
                     SSMRSATELAPPEAVGRYAAERALSRLKARKVKTCEVPVLFESTVAAGLLGAYVQAVS
                     GGALYRKSSFLLDSLGKRVLSRHIDINEDPHVRRGKSSSPFDDEGVVTRAREVVKGGV
                     AQGYFLSSYSARKLGMHTTGHAGGSQNLTLTSRLTHPGDDLAAMLRKLDRGLFVIELM
                     GQGVNQVTGDYSRGAAGFWVERGRIAYPVHEITIAGNLKQMFQNISAVGADAYTMGGK
                     TIGSVLIDSMKIAGS"
     gene            4193..4747
                     /gene="yjgA"
                     /locus_tag="PKV56_13725"
     CDS             4193..4747
                     /gene="yjgA"
                     /locus_tag="PKV56_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HOX68918.1"
                     /translation="MPQNDSADPAETEPIPAWLTRPSKTRRKKDSHDLQDLGVALVEM
                     PDNRLVDLPMDEILLDAIRQYRGTRTHEGRRRQMQYIGKLMRRTDPEPLREAVAAMQL
                     GHAKDALALHDAERWRAELIASDDALTEWTANRPQADVQQLRSLIRAARKDASLVPEQ
                     RSGRAFRELFKFIRQHPAESAEKS"
     gene            4770..5375
                     /gene="mog"
                     /locus_tag="PKV56_13730"
     CDS             4770..5375
                     /gene="mog"
                     /locus_tag="PKV56_13730"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HOX68919.1"
                     /translation="MSETTAPAFDTVRIGVVSISDRASAGIYEDKGVPALKDWLTRAV
                     LNPVNWETRLIPDDLEDIRAALCELVDDVGCDLVLTTGGTGPALRDVTPEATLAVAHK
                     VMPGFGEQMRSISLNFVPTAILSRQVAVIRGKTLIINLPGQPKSIAETLEGLPSATPA
                     IPGIFAAVPYCIDLIGGPYFETDPAVCKAFRPKTAVRPKPQ"
     gene            complement(5407..6174)
                     /gene="cysE"
                     /locus_tag="PKV56_13735"
     CDS             complement(5407..6174)
                     /gene="cysE"
                     /locus_tag="PKV56_13735"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOX68920.1"
                     /translation="MFTRLREDIACIRERDPAARSKLEVLTCYPGLHALALHRIAHAC
                     WLQEWHWLGRFTSHVARFLTGIEIHPGAVIGRRVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLYKGSKRHPTLGKGVVVSAGAKVLGGFTVGDGARVGSNAVLLKPVPPGAT
                     AVGIPARIIEARSEAAREEASARMGFSAYGVTQGDDPVAQAMRGLIDNAAGSAHQIAL
                     LWSAIEALSAAQPGCEVRREPQTTEAFDAEHYKQMMK"
     gene            complement(6265..7035)
                     /locus_tag="PKV56_13740"
     CDS             complement(6265..7035)
                     /locus_tag="PKV56_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX68921.1"
                     /translation="MQTLDFPPAQIADTTRFVLLNTSHPGNVGSTARAMKVMGFTDLV
                     LVSPRYSNVKGKADAISLASGALDVLDGARVVETLAEALDGVTYACATAMTPRDFGPP
                     THAPRDLFQTLVQQPQRVAFVFGSERYGMSNDDVYLCHACLSIPTAPGYGSLNLAQAV
                     QLIAYDWRQALGGFEVVSRTADPALADAVAVQGALDHWRQVLIDIGFHNPQAPQKLMP
                     RLRQLLNRAQLTQEEVHILRGIARAASPAPLASRDKSG"
     gene            7150..8151
                     /locus_tag="PKV56_13745"
     CDS             7150..8151
                     /locus_tag="PKV56_13745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOX68922.1"
                     /translation="MSQALHPMLNIAIKAARTAGALINRASMDVDLLRVSSKAPNDFV
                     TEVDQAAERAIIEVLLAAYPGHGILGEETGSEHGAKDSDYVWIIDPLDGTTNFIHGFP
                     VYAVSIALAFKGQVQQAVVYDPSRNDLFYASKGRGAFLNDKRLRVSKRIRMSESLIGT
                     GFPFRKGDNFKRYVQMFEAVMQNCAGLRRPGAAALDLCYVAAGYYDGFFETGLSPWDI
                     AAGSLLITEAGGLIGNFTGESDFLYQREVVAGNPKIYGQLVQTLAPYTRVIKPEAGST
                     ASASSAAPAASEPEVTPEEALSASLGLTEAVSEPAPKKPTAVRIRKADLKPRGDAPF"
     gene            complement(8152..8859)
                     /locus_tag="PKV56_13750"
     CDS             complement(8152..8859)
                     /locus_tag="PKV56_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX68923.1"
                     /translation="MTVARTPYRSALIVGAGPGLGGSLARVFSRDGGLQVLMAARQPE
                     RLQAVCTEVGSRALRCDATDPDDVAALFDEATRHFGAAPDVVVYNASRRVPGRFVDID
                     AQAMLDSVAVTALGAFHVAQQAARRMLPQGKGAILFTGATAGVKGFAQSASFAMGKFA
                     LRGLAQSLARELGPQGIHVGHVIVDGLIATASSSPAAPDDQLDPDAIAQTYLHLLSQH
                     RSAWTWEVDLRPWSERF"
     gene            complement(8856..9575)
                     /locus_tag="PKV56_13755"
     CDS             complement(8856..9575)
                     /locus_tag="PKV56_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOX68924.1"
                     /translation="MIDLHYWPTPNGHKITMFLEESGLPYRIVPVNIGRGEQFQPDFL
                     RIAPNNRMPAIVDHAPADGGGPLSVFESGAILLYLADKAQRFIAPDLRGRNLALQWLF
                     WQMGGLGPMAGQNHHFVQYAPEKLPYAIDRYMKETARLYAVLNKHLGAMDQAGREYIA
                     GDYSIADMASYPWIVPHEKQGQMLEDFPHLAAWFERVRSRPATVRAYARAKEVSTAPV
                     VDEASRKVLFGQDAASIRSGS"
     gene            complement(9693..10676)
                     /locus_tag="PKV56_13760"
     CDS             complement(9693..10676)
                     /locus_tag="PKV56_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68925.1"
                     /translation="MKSTAQGSSGPLLEAPMRRRLLAAFSQAVWPALALGRPIEADAA
                     DLREQASGQPAAETLSVLRSRLELQFATALSPALQAAARTWVRTSADAVARYFGRFPV
                     PRVDLLLVAEDGRGILGAVTYDEPSLLIRIRLGRETTEVQFLEDQMLVHEMVQLAIPR
                     IPRSQNWLREGVASYVEGVARCRVGLVAPATVWRRWAEEMPQGQPRAGDAGLDHSATW
                     ARARWGGAIFCLLADVELLRRSSQRQGLQQALQGVLAAGGHCGVSWSVDRILSTADAS
                     VGRTTLTDLYRRMKDSPEPVDLDGLWRSLGIAGIAPDEDAPLAKVRRAILS"
BASE COUNT         1867 a         3681 c         3580 g         1812 t
ORIGIN      
        1 tgcaggcgct gccgctgcga atcgtcggca tcatgcagaa cgacaccctg ctggcaatcc
       61 cgttcttcac cttcatgggc ctgatcctcg aacgttcggg catggcggaa gatctgctcg
      121 acacgatcgg ccagttgttc ggcccgatac gcggcgggct ggcctatgcg gtgatcttcg
      181 tcggcgccat gctcgcggcc accaccggcg tggtcgcggc ttcagtgatc tcgatgggac
      241 tgatttctct gccgatcatg ctgcgccacg gctacgaccg gcgtgtcgcc agcggagtga
      301 tcgcggcatc cggcacgctg gctcagatca ttccgccttc gctggtgctg atcgtgctgg
      361 ccgaccaact ggggcaaagc gtgggcgcgc tgtaccaggg cgcgctggtc cccgcactga
      421 cgctcaccgg gttgtacgtc gtctacgttg cggtgatcag cgtggtgaag ccgcagtggg
      481 tgcccgcgat gccggcatcg gcacgcacgc tgcgcgaaga tccgcaggca tcgaccgggc
      541 gatcgcaggt ggctctgttc tggcgggtcg gatgcgcgct ggttccgccg ctggcgttga
      601 tcttcctcgt gctcggcacg atcttcctcg gcgtcgcaac gccgaccgaa ggcggcgcca
      661 tgggagcagt cggcgcattg gtgatggcca tcgtgcgcgg gcgcctcgac atgtccctgc
      721 tgcgtcaggc catgcagagc accgcgaggc tgtcgtgctt cgtgctgttc atcctgatcg
      781 gctcgaccgt cttcagcctg tcgttccagg cggtcgacgg gccgaagtgg gtcgagcacc
      841 tgctgaccgc cctgcccggc ggtgtgctgg gattcctgat cgtcgtgaac ctgctggtct
      901 tcctgctggc gttctttctc gatttcttcg agctcgcctt catcgtggtg ccgctgctcg
      961 ccccggtggc gacggcgctc ggcatcgacc tcgtgtggtt cggcgtgctg ctggcggtga
     1021 acatgcagac ctcattcatg cacccgccct tcggcttcgc gctgttctac ctgcgcagcg
     1081 tcgcgccgga tcgcgaatac accgacacgg tgaccggcca gcggatggcg ccggtcacga
     1141 tcggacagat ctaccgtggc gcgatcccgt tcgtgctgat ccaactggcc atggtggcgc
     1201 tgatcatggc gttcccgggc ctggtgtccc gggggcacga cgtcccggca ggcatcgacg
     1261 ccgatgtgga gttgcgccgg atgcagtccg accagaatgc ggccgagacg gcgaaccccg
     1321 taccggtcac ggcagatggc aactccgccg acgaggaccc gatgaaagcg ctgcagcaag
     1381 ccgtcgaggc cgatcgcaag gactcaccaa agtgacgcgc cgcagcgtca gagcttgatg
     1441 ccctgcatgt agcggtcgaa gctggcttcg gcaaaccgga accacaggtt ctgctcgcgg
     1501 cggaaggctg cgtagtcgtc gtagaccttc ttccagttcg ggttcttcgc acccagctcc
     1561 tcatacagtg ccatggactg cttgaaggcc tcgtcgagca ccgactttgg aaacggcagc
     1621 accttgacgc ccgcgcccac cagtgctttc agcgccgccg ggttctttgc gtcgtagttg
     1681 gcctgcatgt cgacatgcac atgcgacgcg gccatctcga cgatggcctt gtagtcggcg
     1741 ggcaaggcgg cataggcctg cgtgttgatg aagaaatcga tctgcggccc accctcccac
     1801 cagccggggt agtgatagaa cggagcgacc ttgttgaagc ccagcttctg gtcgtcgtaa
     1861 ggaccgaccc attcgaccgc atcgatcgtg cccttttcaa gcgcctggta gacctctccg
     1921 ccggggatgt tctgcgccac tgcgcccatg cgctcgatca cccgcccggc gaatccaccg
     1981 atccgtatct tcaggccctg gatgtcggcc agcgtcttga cttccttgcg gtaccagccg
     2041 cccatctgca ccccggtgtt gccgccgggg aagttgatga tgttgtactt ggcgtagaac
     2101 tcccgcatca gcttcaggcc gttgccgcgg tacatccagg cagtcatctg gcgcgagttc
     2161 aggccgaacg gaatcgccgc accgatcgcg aaggtctcgt ccttgccgaa gtagtagtag
     2221 gggaccgtgt gggccatctc gaccgtggcg gcctgtacgc cgtcgaccac gccgaatggc
     2281 ggcagcaatt cgccggccgg atgcacgctg atctggaact tgccagcggt catctcgccg
     2341 accttcttcg caaacagctc ggcggcaccg aagatggtgt ccagcgattt cgggaaactc
     2401 gatgcaagcc gccagcgcag gttggcctgt gcgtgcacga cggccggcgc tgcgccagca
     2461 gctagcacac cggcgaggcc gctgtgatgg atgaatcgac gacgttgcat gcctcgtgct
     2521 cctgtgactc ttcggacccc gctcttccca gtctggatgc agcgcagatc agctgccggc
     2581 gatcttcatc gagtcgatca gcaccgaacc gatggtcttg ccgcccatcg tgtaggcgtc
     2641 ggcgccgacg gcggagatgt tctggaacat ctgcttcagg ttgcctgcga tggtgatctc
     2701 gtgcaccgga tacgcgatgc gaccgcgctc gacccagaac ccggccgctc cccgggaata
     2761 gtcgccagtg acctggttga cgccctgccc catcagttcg atgacgaaca ggccgcggtc
     2821 gagcttgcgc agcatcgcgg ccagatcgtc gcctggatgg gtgaggcggc ttgtcagcgt
     2881 gaggttctgc gatccgccgg catggccggt ggtgtgcatc ccgagcttgc gggccgagta
     2941 gctcgacagg aagtaaccct gggcgacgcc acccttgacc acctcccggg ctcgggtgac
     3001 cacgccttca tcgtcgaacg gcgaactgct cttgccgcgc cgcacgtgcg ggtcttcgtt
     3061 gatgtcgatg tgcctggaaa gcaccctctt gcccaggctg tcgagcagga agctcgactt
     3121 gcgatacagc gcaccgccgc tgacagcctg cacataggca ccgagcaagc cggccgcgac
     3181 cgtcgactcg aacagcacag gcacttcgca ggtcttgacc ttgcgcgcct tcagccgcga
     3241 aagcgcgcgc tcggcggcgt agcggcccac cgcttccggc ggggccagct cggtggcaga
     3301 acgcatcgag ctgtaccagg catcgcgctg catgtcgtcg ccgttgcggc cgggcaatga
     3361 ggcaatcggg gcaaccgaca gcgaatgccg cgaactggcg tagccgccgc gaaagccgcg
     3421 actgttgccg gcaaagaagt gcgactgctg tgccgaaacg cccgcaccct cggaattggt
     3481 gatgcgcttg tcgacggaca gcgccgcgct ctcgcagcgc ctggcaagat cggcagcacc
     3541 ttccgcatcg atgtcccacg gatggaacag atccaggtcg agcgtggcga catcgccgaa
     3601 ggccaggtct gccgtgtcgg gcagcccggc cgcgggatcc tcggcggtga agcgcgcgat
     3661 gtcgtgtgct gcgcgcaccg tctgctcgag cgctgcggga gagaaatcgg acgtgctcgc
     3721 gttgccgcgg cgctggccca catagaccga gacaccgatc gacttgtctc gattccgctc
     3781 gacgttctcg atttcgccct tgcgcaccga caccgacagg ccgacgcctt ccgacacctc
     3841 ggctccggcg tcggtggcac cgagcgaagc cgccagtttc agcgcgtcgt cgacgagttg
     3901 ctggaaatcc gcgcgggaat aggcgaatcc ggtggacgaa gatgacggcg aggtcgtgct
     3961 gggcatgggt tgtggacgca gggtgaggtg ctgggtgcgg caatgccgta tcgggtcgga
     4021 ttcgcgcggg catcgtcgca tcgacggaaa tgccgtgccg ctgcaggccg ggcgcagagg
     4081 ccgaaacctc gtggctatga taccgagcgc ttgcacgcaa aggccgctgt cctgcacatc
     4141 gcccagcgcc ccgactcgac cggcgcgcaa ccacgcaatt tctcatcccg acatgccaca
     4201 gaacgactcc gccgaccctg cagaaaccga accgatcccg gcgtggctga cccgtccgag
     4261 caagacgaga cgcaagaagg attctcacga cctgcaggat ctgggcgtgg cgctggtcga
     4321 gatgccggac aaccggctgg tcgatctgcc gatggacgag atcctgctgg acgccatccg
     4381 gcagtaccgg ggcacccgca ctcacgaagg caggcgccgc cagatgcagt acatcggcaa
     4441 gctgatgcgc cggaccgatc ccgaacccct gcgcgaggcg gttgccgcga tgcaactggg
     4501 ccacgccaag gatgccctgg cattgcacga tgccgagcgt tggcgcgccg aactgattgc
     4561 cagcgacgac gcgctgaccg aatggacggc gaaccgcccg caggccgatg tgcagcagct
     4621 gcgcagcctg atccgcgcgg cacgcaagga cgcctcgctg gtacccgagc agcgcagcgg
     4681 gcgcgccttc cgggagctgt tcaagttcat acgccagcat ccggccgaat cggccgagaa
     4741 gagctgacga caaccgacac gagaccccca tgagcgaaac caccgccccc gccttcgaca
     4801 ccgttcgtat cggcgtcgtg tcgatcagcg accgcgcctc ggccggcatc tacgaggaca
     4861 agggcgtgcc ggcgctcaag gactggctga cgcgcgccgt gctgaatccg gtgaactggg
     4921 aaacccgcct gatccctgat gatctggagg acatccgagc cgcgctctgc gaactggtcg
     4981 acgacgtcgg ctgcgacctc gtgctgacca ccggtggcac cggcccggca ctgcgcgacg
     5041 tgactccgga ggcgacgctg gccgtggccc acaaggtgat gccgggcttc ggcgagcaga
     5101 tgcgcagcat cagcctgaat ttcgtgccga ccgcgatcct gtcgcggcag gtggccgtca
     5161 tccgtggcaa gacgttgatc atcaacctgc ccggccagcc gaaatcgatc gccgagacac
     5221 tggaaggact gccttccgcc accccggcca tcccgggcat cttcgcggcc gtgccctact
     5281 gcatcgacct gatcggcgga ccgtacttcg agaccgatcc ggcggtctgc aaggcgttca
     5341 ggccgaaaac agcagttcga ccgaaacccc agtgacgcac gccgttcggc cgttgcgtca
     5401 aactgctcac ttcatcatct gcttgtagtg ctcggcatcg aaggcttcgg tcgtctgcgg
     5461 ttcgcgacga acctcgcagc caggctgggc ggccgacagc gcctcgatgg cggaccacag
     5521 cagcgcaatc tggtgcgccg acccggccgc gttgtcgatc agtccgcgca tggcctgcgc
     5581 caccggatca tcaccctgcg tcacgccgta ggcagaaaag cccatgcggg ccgatgcctc
     5641 ctcgcgggcc gcctctgacc gtgcctcgat gatgcgagcc ggtatgccca ccgccgtggc
     5701 gccgggaggc accggcttca acagcaccgc gttcgatccg acccgtgcgc catcgcccac
     5761 ggtgaaccca cccagcacct tggctccggc gctgacgacc acgcccttgc ccagcgtcgg
     5821 gtgacgcttg ctgcccttgt agagcgaggt gccaccgagg gtcacgccct ggtagatcgt
     5881 gcagtcgtcg ccgatttcgg cggtctcgcc gatcacgacg cccatgccgt gatcgatgaa
     5941 gacccggcgc ccgatcacgg cgcccgggtg gatctcgatc cctgtcagga agcgcgccac
     6001 atgggaagtg aaacgtccca gccaatgcca ctcctgcagc cagcaggcgt gcgcgatccg
     6061 atgcagcgcg agtgcatgca ggcccgggta gcaggtgagc acctccagct tcgagcgggc
     6121 cgccgggtcg cgttcgcgga tgcaggcgat gtcttcgcgc aggcgtgtga acatggaaat
     6181 gcgaaaaacg aatgaggccg cgagtttagc gacgcggctg cggagtcggc cggggtgcgg
     6241 atatgcggca ggctggacgg ctcctcagcc agacttgtcc ctgcttgcca gtggcgccgg
     6301 cgaggcagca cgcgcgatgc cgcgcaggat gtgcacctcc tcctgcgtca gctgcgcgcg
     6361 gttgagcaac tggcgcagcc gcggcatcag tttctgcggc gcctgcgggt tgtggaagcc
     6421 gatgtcgatc agcacctgtc gccaatggtc gagcgcgccc tgcacggcca ccgcgtcggc
     6481 cagagccgga tcggcagtgc gcgaaaccac ctcgaagcca cccagggcct gccgccagtc
     6541 gtaggcgatc agctgcaccg cctgcgccag gttcagcgac ccatagcccg gcgcggttgg
     6601 aatgctgaga caggcgtggc acaggtagac atcgtcgttg ctcatgccgt agcgctcgga
     6661 gccgaacacg aacgcgacac gctgcggctg ctgcaccagc gtctggaaca ggtcgcgcgg
     6721 cgcatgggtg ggtggaccga aatcgcgtgg cgtcatcgcg gtggcacagg cataggtcac
     6781 cccgtcgagt gcctcggcga gtgtctccac gacacgcgcg ccatccagga catcgagtgc
     6841 gccactggcc agcgagatgg cgtcggcctt gcccttgacg ttcgaatagc gaggtgaaac
     6901 cagcaccaag tctgtgaagc ccatcacctt cattgcacgc gcggtcgacc ccacattgcc
     6961 cgggtggctc gtgttgagca gcacgaagcg cgtggtgtcg gcgatctggg ccggcgggaa
     7021 atcgagggtt tgcatggggt ggccgctaaa atcacgatta tcggaagtca gcgacgcgcg
     7081 cgatcgcgcc gccggactcc gaaccgctct ttctccccgc cagtccccac cacaccacag
     7141 ggttgcacca tgtcgcaagc gctccatccg atgctcaaca tcgccatcaa ggcagcacga
     7201 acggccggtg cattgatcaa ccgggcatcg atggatgtcg acctgctgcg cgtcagcagc
     7261 aaggcgccga acgacttcgt caccgaagtc gatcaggccg ccgaacgggc gatcatcgaa
     7321 gtgcttctgg cggcctatcc cggccacggg atcctcggcg aggaaacggg cagcgagcat
     7381 ggtgccaagg acagcgacta cgtctggatc atcgatccgc tcgacggcac caccaacttc
     7441 atccacggct tcccggtcta cgcggtgtcg atcgcgcttg ccttcaaggg gcaggtgcag
     7501 caggccgtcg tctacgaccc gtcgcgcaac gacctgttct acgcatcgaa ggggcgcggc
     7561 gcgttcctga atgacaagcg cctgcgcgtg tccaagcgca tccgcatgtc cgaatcgctg
     7621 atcggcacgg gcttcccgtt ccgcaagggc gacaacttca agcgctacgt gcagatgttc
     7681 gaggcggtga tgcagaactg cgccggactg cgccgcccgg gagccgccgc gctcgacctc
     7741 tgctacgtgg cggcgggcta ctacgacggc ttcttcgaga ccggcctgag cccgtgggac
     7801 atcgccgccg gctcgttgct gatcaccgag gcgggcggcc tgatcggcaa tttcaccggc
     7861 gaatccgact tcctgtacca gcgcgaggtg gtggccggca acccgaagat ctatgggcag
     7921 ctggtgcaga cgctggcacc gtacacccgc gtgatcaagc ctgaagcagg ctcgaccgca
     7981 tcggcatcct cggcagcgcc ggctgcaagc gagcctgaag tgacccccga agaagcgctg
     8041 tcggcttcgc tggggctgac cgaggccgtc agcgagcccg caccaaagaa gccgaccgct
     8101 gtgcgcatcc gcaaggccga cctgaagccc cggggcgacg cgccgttctg atcagaagcg
     8161 ctccgaccag gggcgcaggt cgacctccca cgtccaggcg ctgcggtgct gcgacagcag
     8221 gtgcaggtag gtctgcgcga tcgcgtcggg gtcaagctga tcgtccggcg ccgccggtga
     8281 cgacgacgcc gtggcgatca gcccgtcgac gatcacgtgc ccgacgtgga tgccttgcgg
     8341 cccgagttcg cgggcgaggc tttgcgccag acctcgcaag gcgaacttgc ccatcgcgaa
     8401 cgaggcggat tgcgcaaacc ccttgacgcc ggcggtcgcg ccggtgaaca ggatggcgcc
     8461 cttgccctgc ggcagcatgc gacgcgctgc ctgctgcgcg acgtggaaag cgcccagcgc
     8521 agtcaccgcc accgaatcca gcatggcctg ggcatcgatg tcgacaaaac gtccgggcac
     8581 gcgccgactc gcgttataga cgaccacgtc gggcgccgcg ccgaaatgcc gcgtggcctc
     8641 gtcgaacagg gctgccacgt cgtccggatc ggtggcgtcg cagcgcaagg cacgggatcc
     8701 gacctcggtg cacaccgcct gcagcctttc gggctgccgc gcagccatca acacctgcag
     8761 gccgccgtcg cgactgaaga cgcgggccag gctgccgccg agacccggtc cagcgccgac
     8821 gatcagcgcg cttcgatagg gtgtgcgtgc gaccgtcatg aaccactccg gatgctcgcc
     8881 gcgtcctgcc cgaacagcac cttcctggat gcctcgtcga ccaccggcgc cgtgctgact
     8941 tccttcgcgc gggcataggc ccgcaccgtg gcgggacggc tgcgcacccg ctcgaaccac
     9001 gccgcgaggt ggggaaagtc ttccagcatc tggccctgct tctcgtgcgg aacgatccac
     9061 ggatagctcg ccatgtcggc gatcgaatag tcgccggcga tgtactcgcg gcctgcttgg
     9121 tccatcgcgc ccaggtgctt gttcagcacc gcgtacagcc gcgcggtctc cttcatgtag
     9181 cggtcgatcg cgtagggcag cttctccggc gcgtactgca cgaagtgatg gttctggccg
     9241 gccatcggcc ccaacccgcc catctgccag aacagccact gcagcgcgag gttgcggccc
     9301 cgcaggtcag gtgcaatgaa acgctgcgcc ttgtcggcca ggtacagcag gatcgcgccg
     9361 gactcgaaga ccgacagcgg cccgccgcca tcggccggtg cgtggtcgac gatggccggc
     9421 atgcggttgt tcggggcgat gcgcaggaag tcgggctgga actgctcgcc gcggccgatg
     9481 ttcaccggca cgatgcggta cggcaggccc gattcctcga ggaacatcgt gatcttgtgg
     9541 ccgttgggcg tgggccagta gtgcaggtcg atcatgtcgc gggtccgatc aatcatcagt
     9601 cttgggtgcc gcgtcagatc gagcggcgtg gtggacattc tgagaggact gcatcggccg
     9661 cgcccaggaa ggggctcggc cgggttcggc gttcaggaca ggatcgcacg acgtaccttg
     9721 gccagcgggg cgtcttcgtc aggcgcgatg ccagcgatgc ccaggctgcg ccagagtccg
     9781 tccagatcga ccggttcggg cgagtccttc atgcgccggt acagatcggt cagcgtcgtg
     9841 cggcccaccg acgcatccgc ggtgctcagg atgcgatcga ccgaccagga tacgccgcag
     9901 tggcctccgg cagccagcac accctgaagc gcctgctgca gaccctggcg ctgcgagctg
     9961 cgcctgagca attccacatc ggcaagcagg cagaagatgg cgccgcccca gcgggcgcga
    10021 gcccaggtcg ctgagtgatc caggccagcg tcccctgccc tgggctggcc ctggggcatc
    10081 tcttcggccc agcggcgcca gaccgtggcc ggggcaacca ggccgacacg gcagcgcgcc
    10141 acaccttcga catagctggc aacaccctcg cgcagccagt tctgcgaccg cgggatgcgc
    10201 ggaatggcga gctgcaccat ttcatgcacc agcatctggt cctcgaggaa ctgaacctcc
    10261 gtggtctcgc gccccagccg gatgcggatc agcagagacg gctcgtcgta ggtgaccgcg
    10321 cccagaatgc cgcggccgtc ctccgcaacc agcagcagat cgacccgcgg caccggaaat
    10381 cgcccgaaat agcgggccac cgcgtcggcc gaggttcgca cccaggtgcg cgccgcagcc
    10441 tgcaatgcag ggctgagggc cgttgcgaac tgcagttcca gccgcgatcg gagcaccgac
    10501 agggtctccg ccgccggctg gccgctggcc tgttcacgca agtcggccgc atcggcctcg
    10561 atgggccgac ccagggccag ggcgggccac acggcctgcg aaaacgcagc aagcaacctg
    10621 cgtcgcatcg gtgcttcaag cagcgggccg gacgaaccct gtgctgtcga tttcatcgag
    10681 tctcctggag cgaacggact ggatcgaggg cggaaacagg accttaccgg catctgcacc
    10741 gcaactgcgc acggaccaca ccaaaccgca agtgcctccg gtcacaccca ccagctccag
    10801 ctttcgatgt gtgaagatcg cgcccgcacc gagatcatct cagcagcgaa ggcatgttcg
    10861 atgcttgcat cggcaggttg caagttcgga tgcgatgttc ggggcgtggc gcgggaatgg
    10921 ggtggcaagc gatgtggtcg
//
LOCUS       DAOORV010000117        10896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7531_length_10896_cov_7.073333, whole genome shotgun sequence.
ACCESSION   DAOORV010000117 DAOORV010000000
VERSION     DAOORV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7531_length_10896_cov_7.073333"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3630
                     /gene="dnaE"
                     /locus_tag="PKV56_13765"
     CDS             <1..3630
                     /gene="dnaE"
                     /locus_tag="PKV56_13765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX68926.1"
                     /translation="FVHLRTHTEYSVVDGTLRIDEVVASAAADRQVALAITDLGNLFG
                     AIKFYNAARSAGIKPVIGADVWLEPESSDRPPSRLLLLAQNRAGYLNLCELLTRCWMG
                     PASQAQPWTTWDVLSELNGGLIALSGADQGAVGQALVAGDTARARKLARRFADVFSNR
                     YYLELQRAGLPGQEPHVQAAVRLASELQLPVVATHPVQFLSGDDFEAHEARVCVAEGE
                     TLTNPRRIKRFNAQQYFKTQAEMEALFADVPSALANSVEIAKRCSLMLVLGKPQLPAF
                     PTPEVNGERMAPEAFFRHSSHEGLNARLVQLYPDAARREAERGRYVERLEFEIATILK
                     MGFPGYFLIVADFINWAKANGCPVGPGRGSGAGSLVAYALKITDLDPLQYNLLFERFL
                     NPERVSMPDFDIDFCQANRDRVIDYVKDKYGRDAVSQIVTFGTMAAKAALRDIGRVLG
                     MGYGHVDSIAKLVPAPPGKTVTLRRPPNDTDTSVIYARREAPEIEEREQKEEEVAELL
                     ALAERVEGIVRNVGMHAGGVLIAPGKITDFCPLYMQPGSDSAVSQFDKDDVESIGLVK
                     FDFLGLATLTILEMAKEFIRARHPDQREFAFENLPLDDAGAYRLMSEGKTVAVFQLES
                     SGMQRMLRDAKPSVFEDVIALVALYRPGPMDLIPSFCARKHGREEVEYPHPLMREVLE
                     ETYGIMVYQEQVMQVAQIVGGYSLGGADLLRRAMGKKKLEEMMKHRAVFAEGAAKKGI
                     AEPKANEIFDLMEKFAGYGFNKSHAAAYALLAYHTAWAKVHYLAEFTAANMSVAIDDT
                     DKLKIFHDDAVALGIVFEPPDVNAGGYRFEPITPVAGTRQKGERGRVRYGLGAIKGTG
                     QGAIEAIVAARAEGGPFTSLFDFCTRVDRTRINKRTVEALIKAGAFDGWGIEPRGALA
                     GVDPLAGQARQGGDPGAPGSQRSALLASVGLAFDFADSQAAHANQGGLFDFDDAHGAS
                     TQEPPLVEALPWSIKERLMAEKTALGFYLSGHLFDQSVDEVRQFARRCNADVLDSREP
                     QLLAGIVGDLRMVNGQRGRVAIFKLDDKTEAIEAVASEDLVNANRELLRDDELVIVQG
                     KVQPDRFSGGLRLNVQQVWGLAEARCRFGKYLSVQVNGVPPPVAELLRDFPVRRVLTE
                     HGELPQGLSVRLHLRRRADQGEVTGDLDLGEQARFYPSDEAIERWRVAADGRRARVVY
                     DASAG"
     gene            complement(3754..4134)
                     /locus_tag="PKV56_13770"
     CDS             complement(3754..4134)
                     /locus_tag="PKV56_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C552"
                     /protein_id="HOX68927.1"
                     /translation="MRMLLSATAALMIVNAPAQANPAENDQAMIKLATDSGCMACHSI
                     LPLPKRADGLPPIAPAWRDIAIKYRDDPLASKRLSKTVMTGSNPKSSHWTGKIGTVSM
                     PPNAVAISEAKARMLVNWLLILVP"
     gene            complement(4235..6964)
                     /locus_tag="PKV56_13775"
     CDS             complement(4235..6964)
                     /locus_tag="PKV56_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX68928.1"
                     /translation="MTSAGAAMGERAAPSEVRPHKLFAPPVYPGAVPRQVILDRVEHD
                     DSLRLTVLQGPAGHGKSTTLQQIKSAQAARGWRTAWLTFDDADNDPRRFESHMVAMMG
                     RLTDGATASAAPRTGAGDAPRDLADWMLDTLSGLDMQASIFLDEFQALRGEAILRFFR
                     TLLARLPPHVHVFIGSRSLPEIGLATLLVNRVAIVVRADDLRFTPDEVAKFFAGSNNL
                     RVSADEVDAIYRRTEGWPAGVQLFRLALLSPEVRLSLGGDEDSGPRELAEYLADNVMS
                     LQTARIQEFLLRTSLLQRLSAPLCEAVTGFDDAQSLLEQLERSGLFVRALDSDTRWFK
                     YHGLFSSYLADSLQRSAPDEVRRVHEQAARWYLLNAMPEEAVHHALCCGDFALAASTL
                     TDWSAARVAAAELITFERWFDRLPFHEVAQHAALVIRAAYALMFLRRRPKLRPLLDLM
                     SAQAACGDIVPTTNPDLCRAMSFLLVDDDMPAALAIIDQPGVVQREFDGFPAFELGAA
                     SNVLAMGKISRGDFEGARQALALARAHIGRGGGSFVGGYTAAITGSNLLVQGRLHEAL
                     AHLREAHAQETPLDTSVAGAALAACHIWALYEANDLETLESLAHRFQQEISESVTLDF
                     IAVAHLAIARMHEARGRSETALAVLDELERIGHEAPWKRLVAVSEWERVRRALAAGDI
                     ERADALAARIAPDAMSQDPQWIHMSEDMEGSGYGLIRLAIARNDFVDAARRIAAERMR
                     QRGRVYRDIKLSVLEALLHHGSGARNSAQRSLRRALQLGRKGAYVRCLLDEGEGVVEL
                     LREAYQKILQANEPGGAAADPDRAYIEHLLAASGSGLGRQHVRTHLMERLSERERDML
                     LLLVNGITNREIAGRLFVSENTVKFHLKNIYSKLGVGNRLQAINTARALRLIE"
     gene            7233..8852
                     /locus_tag="PKV56_13780"
     CDS             7233..8852
                     /locus_tag="PKV56_13780"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX68929.1"
                     /translation="MSNVQQAPKQFDAVVVGAGVAGLYSIYKLREQGLSVQAFEAGTG
                     VGGTWYWNRYPGARVDSQAYVYQYWFSDDLLNEWDWSERFPAQEETERYLNHVADKFD
                     LRSNIRFKTRVTGASFDESTQRWIVATDDGQTVSAQFLIMGTGGLSVPMLPPFPGRER
                     FKGQIVHTAQWPKDGVDLKGKRVGVIGTGATGIQVIQTIADQVGHLTVFQRTANYTIP
                     MRNPKYDDADRSELRSRYPDLKKRVHGTFAGFDYDFDSTDFLSLSAEDRQKKMQALWD
                     DGSLSFWVGSFLSVFVDEKVNEEFSEFVRQRIRARVRDPKVAEMLVPNDHGFGTRRVP
                     LETKYYEVFNRDNVTLVDTRAAPIECLTEKGLQTAAGEHELDIIIFATGFDAGTGALT
                     KTDIRGRNGLLLRDAWEADGIRTTMGLQVHGYPNLFMTMAPFSPAAAFCNVPTCVQQQ
                     VDWITDCIRFVRDQGRRSIEPSEAVEAQWVAHHDELANMMLVSKTNSWYMGSNVEGKQ
                     RRLLAYPGGVGNYRDKCDAVKASGYEGFVTA"
     gene            complement(8924..9598)
                     /locus_tag="PKV56_13785"
     CDS             complement(8924..9598)
                     /locus_tag="PKV56_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOX68930.1"
                     /translation="MARTFIRPLLLACLAWGSAQAQAGVVEFQGFANGSQTVNYQLSA
                     PNPSANGSTSAGGFKVLLDGTTSVTTYCIDLLQHISLGGSYNDYTEVAGSAHVFANTR
                     AHDDIGRLFSAGHEVNDATEQAAFQIAIWELAYETSPTSYDVDSGAARFFGGTAANGA
                     LALADDWLSNLGSINNVSVRVLESPSHQDQVFANPVPEPSSAALIMAAMLSLGFVQRR
                     RSSRDG"
     gene            9849..10664
                     /locus_tag="PKV56_13790"
     CDS             9849..10664
                     /locus_tag="PKV56_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOX68931.1"
                     /translation="MAMDVVDYEIKGAEMQFVEVELDPGEAAVGEAGSMMFMDAGISM
                     DTVFGDGSASTGGLFGKLLGAGKRLVTGESLFTTVYTNQGHSKLRIAFAAPYPGKILP
                     MDLRKLGGMLICQKDAFLCAAKGVSLGIYLQQKMSVGFFGGEGFIMQKLEGDGLAFVH
                     AGGTVLKRELQPGQTLLIDTGCVVAFTPDINFEIQYVGKIKTALFGGEGLFFAKVTGP
                     GTVWLQSLPFSRLASRVFAAAPQRGGSREEGSVLGGVLGGGLLGGSIFGGDDE"
BASE COUNT         1827 a         3490 c         3717 g         1862 t
ORIGIN      
        1 ttcgtccacc tgcgcacgca caccgaatac tccgtcgtcg acggcaccct gcggatcgac
       61 gaggtggtgg cctccgccgc ggccgaccgg caggtggcgc tggccatcac cgacctcggc
      121 aacctgttcg gtgcgatcaa gttctacaac gccgcgcgtt cggcaggaat caagccggtg
      181 atcggcgccg atgtctggct ggaacccgag agcagtgatc gcccgccgag ccgtctgctg
      241 ctgctcgctc agaaccgcgc cggctacctg aacctctgcg aactgctgac ccgctgctgg
      301 atgggcccgg cctcgcaggc ccagccctgg acgacctggg acgtgctgtc ggagctgaac
      361 ggcgggctga tcgcgctgtc cggcgccgat cagggggccg tcggccaggc gctggtcgcg
      421 ggtgacacgg cgcgcgcgcg gaagctggcc cggcgatttg ccgatgtgtt ttcgaaccgc
      481 tattacctgg agctccagcg cgctggcctg cccgggcagg agccccacgt gcaggctgcg
      541 gtgcggctgg cctccgagct gcagttgccg gtggtcgcca cgcacccggt gcagttcctg
      601 agcggcgacg acttcgaggc ccacgaggcg cgcgtctgcg ttgccgaggg ggagacgctg
      661 acgaatccgc gccgcatcaa gcgcttcaat gcgcagcagt atttcaagac ccaggccgag
      721 atggaagcgc tgttcgccga cgtgcccagc gcgctggcca actccgtcga gatcgcgaag
      781 cggtgcagcc tgatgctggt gctcggcaag cctcagctgc cggccttccc gacgcccgag
      841 gtcaacggcg agcgcatggc gcccgaggcg ttctttcgtc attcgtcgca cgaaggactg
      901 aacgcgcgtc tggtccagct ctatcccgat gccgcaaggc gcgaggccga acgtggccgc
      961 tacgtcgagc ggctcgagtt cgagatcgcc acgatcctca agatgggctt cccgggctac
     1021 ttcctgatcg tggccgactt catcaactgg gccaaggcca acggctgccc ggtcggaccc
     1081 ggccgcggtt cgggcgccgg ctcgctggtc gcgtatgcgc tgaagatcac cgacctcgac
     1141 ccgctgcaat acaacctgct gttcgagcga ttcctgaatc cggagcgggt gtcgatgccc
     1201 gacttcgaca tcgatttctg ccaggccaat cgcgatcgcg tcatcgatta cgtcaaggac
     1261 aagtacggcc gcgacgcggt gagccagatc gtcaccttcg gaacgatggc cgccaaggcc
     1321 gcactgcgcg acatcggccg tgtgctcggc atgggttacg gccacgtcga cagcattgcc
     1381 aagctggtgc ccgcgccacc cggcaagacg gtgacgctgc gccggccgcc caacgacacc
     1441 gacaccagcg tgatctacgc ccggcgcgag gcgcccgaga tcgaggagcg cgaacagaag
     1501 gaagaggagg tcgccgaact gctggcgctt gccgagcgag tcgaaggcat cgtgcgcaac
     1561 gtcggcatgc atgcgggcgg tgtgctgatc gcgccgggca agatcaccga cttctgcccg
     1621 ctgtacatgc agcccggcag cgacagcgcg gtgagccagt tcgacaagga cgacgtcgaa
     1681 tcgatcggcc tggtcaagtt cgacttcctg ggtctggcca cgctgaccat cctcgagatg
     1741 gccaaggagt tcatccgcgc gcgccatccg gatcagcgcg agttcgcctt cgagaacctg
     1801 ccgcttgacg acgcgggcgc ctaccggctg atgagcgagg gcaagacggt ggcggtgttc
     1861 cagctggaaa gctcgggcat gcagcgcatg ctgcgcgacg ccaagcccag cgtgttcgag
     1921 gatgtgatcg cactggtggc gctgtaccgc ccgggcccga tggacctgat cccgagcttc
     1981 tgcgcccgca agcacggtcg agaggaggtc gagtatccgc atccgctgat gcgcgaggtg
     2041 ctcgaggaaa cctacgggat catggtctac caggagcagg tgatgcaggt ggcccagatc
     2101 gtcggcggct actcgctggg tggcgccgac ctgctgcgcc gcgcgatggg caagaagaag
     2161 ctcgaggaga tgatgaagca ccgggccgtc ttcgccgagg gcgcggccaa gaagggcatc
     2221 gcggagccga aggccaacga gatctttgac ctgatggaga agttcgcggg ctacggcttc
     2281 aacaagtcgc atgcagccgc ctacgcgctg ctggcgtatc acaccgcctg ggccaaggtg
     2341 cactacctgg ccgagttcac cgccgccaac atgagcgtgg cgatcgacga caccgacaag
     2401 ctgaagatct tccacgacga tgcggtggcg ctgggcatcg tgttcgagcc gcccgacgtg
     2461 aatgccggcg gataccgctt cgagccgatc acgccggttg ccgggacccg gcagaagggc
     2521 gagcgcggcc gggtgcgcta cggcctcggc gcgatcaagg gcaccgggca gggcgccatc
     2581 gaggccatcg tcgcggcgcg cgctgaaggg ggcccgttca ccagcctgtt cgacttctgc
     2641 acccgtgtcg atcgcacccg catcaacaag cgcaccgtcg aggcgctgat caaggccgga
     2701 gccttcgatg gctggggaat cgagccacgg ggcgccctgg ccggcgtcga ccccctcgca
     2761 gggcaggcaa ggcaaggtgg cgatccaggg gctcccggct cacagcgttc ggcgttgctg
     2821 gcgagcgttg gtctggcttt tgatttcgcc gattcccagg ctgcgcatgc caatcagggt
     2881 ggcctgttcg acttcgatga cgcgcatggc gcttcgacac aggaaccgcc gctggtcgaa
     2941 gcattgccct ggagcatcaa ggagcgactg atggccgaga agaccgcact cggcttctac
     3001 ctgtcgggcc acctgttcga ccagagcgtc gacgaggtgc ggcagttcgc caggcgctgc
     3061 aatgccgatg tgctcgacag ccgcgagcca cagctgctgg ccggcatcgt cggtgacctg
     3121 cgcatggtca acggacagcg tggccgcgtg gcgatcttca agctcgacga caagaccgag
     3181 gccatcgagg cggtggccag cgaagacctg gtgaacgcca accgcgagtt gctgagggac
     3241 gacgaactcg tcatcgtcca gggcaaggtg cagcccgacc gcttctcggg cggcttgcgt
     3301 ctcaacgtgc agcaggtctg gggtctggcc gaggcgcgct gccggttcgg caagtacctg
     3361 agcgtgcagg tcaacggggt gcctccgccg gtggccgaac tgttgcgtga ctttccagtg
     3421 cggcgcgttc tcaccgaaca cggcgagctg ccgcaggggc tgtccgttcg gctgcatctg
     3481 aggcgccggg ccgatcaggg cgaagtcacc ggggatctcg acctgggcga gcaggctcgc
     3541 ttctatccca gcgacgaggc gatcgaacgc tggcgcgtgg cggccgacgg acggcgggcg
     3601 agggtggtct acgacgcatc cgcagggtga cgtttcagtc gatcctggcg gatcaggcgc
     3661 gctccgcact cacgccgtgc ctgccaggcg gcctggccgt gccacgggca tccagctgca
     3721 aaggcggatc cgtgctgccg aaggggcggg ccgctacggc accaggatca gcagccagtt
     3781 gaccagcatg cgcgccttcg cttcgctgat ggcgaccgcg ttcggcggca tcgagacggt
     3841 gccgatcttc cctgtccagt gactggactt cggattcgac ccggtcatga cggttttcga
     3901 cagccgtttc gacgcgagcg ggtcgtcccg gtacttgatg gcgatgtcgc gccatgccgg
     3961 tgcaatgggt ggcaggccgt ccgcgcgctt gggcaacggc aggatgctgt ggcaagccat
     4021 gcagccactg tcggtggcga gcttgatcat ggcctgatcg ttctcggccg ggttcgcctg
     4081 tgccggcgcg ttgacgatca tcagcgcagc ggtggcgctc agcagcatac gcatctggaa
     4141 ttcctgtaat ggttggccca agcctacatg ctgccagccg gcttgcagct tgacgcagct
     4201 caatcgccgc agggagggtt cgattcgcat cgcctcactc gatcagccgc agtgcccttg
     4261 ccgtgttgat cgcctgcagg cggttgccga ctcccagctt cgaatagatg ttcttcaggt
     4321 ggaacttgac cgtgttctcc gagacgaaga gccgccctgc gatttcccgg ttggtgatgc
     4381 cgttgacgag cagcagcagc atgtcgcgct cgcgctccga caggcgttcc atcagatgcg
     4441 tgcggacatg ctggcgtccg aggcccgatc ccgaggctgc gagcaggtgc tcgatgtagg
     4501 cgcggtccgg gtcggcggcg gcgccccccg gctcgttggc ctgcaggatc ttctgatacg
     4561 cctcgcgcaa cagttcgacc acgccttcgc cctcgtcgag caggcagcgc acgtaggcgc
     4621 ccttgcgacc cagctgcagt gcacggcgca ggctgcgctg cgccgagttg cgcgccccgc
     4681 tgccgtgatg cagcagcgct tccagcacgg acagcttgat gtctcggtag acgcgtccgc
     4741 gctggcgcat ccgctcggcg gcgatgcgcc gcgctgcgtc gacaaagtcg ttgcgcgcga
     4801 tggccagcct tatcagccca tagcccgagc cctccatgtc ctcggacatg tggatccatt
     4861 gcgggtcctg cgacatcgcg tcgggggcga tgcgcgcggc cagtgcatcg gcacgctcga
     4921 tgtcacccgc ggcgagtgcg cgccgcacgc gctcccattc gctgacggcc accagccgct
     4981 tccagggagc ctcgtgcccg atgcgctcga gttcgtccag cacggccagg gctgtctccg
     5041 agcgcccacg cgcttcgtgc atccgggcga tcgcgaggtg ggccaccgcg atgaagtcca
     5101 gcgtgaccga ctccgaaatt tcctgctgga atcggtgcgc gagcgattcg agagtctcga
     5161 ggtcgttggc ttcgtacagt gcccagatgt ggcaggccgc gagcgcggcc ccggccaccg
     5221 aggtgtcgag cggcgtctcc tgcgcgtggg cttcgcgcag atgagccagc gcctcgtgca
     5281 accgaccttg aacgagcagg ttgctgccgg tgatggctgc ggtgtagccg ccgacgaacg
     5341 agcctccgcc gcgcccgatg tgggcgcgcg ccagggccag tgcctggcgt gcgccttcga
     5401 agtcgccgcg cgagatcttg cccatggcca gtacgttcga tgcggcgccg agctcgaacg
     5461 ccggaaagcc gtcgaactcg cgctgaacca cgccgggctg gtcgatgatc gccagtgccg
     5521 cgggcatgtc atcgtcgacc agcaggaagg acatcgcccg gcacagatcg gggttggtcg
     5581 tgggcacgat gtcgccgcac gctgcctgcg cggacatcag gtctagcagt ggccggagct
     5641 tgggacggcg ccgcagaaac atcaatgcat acgccgcgcg gatcaccagc gcggcatgct
     5701 gagccacctc gtggaacggc aggcgatcga accagcgttc gaaggtgatc aattcggctg
     5761 cggccacgcg cgcggcggac cagtcggtca gcgtcgatgc cgcgagcgcg aagtcaccgc
     5821 agcacagcgc gtggtggacc gcctcttcag gcatggcgtt gagcaggtac caccgcgccg
     5881 cctgttcgtg gacgcggcgc acctcgtcgg gtgcacttcg ctgcaggctg tcggccaggt
     5941 aactcgagaa cagcccgtgg tacttgaacc accgggtgtc cgaatcgagg gcgcgcacga
     6001 acaggcccga ccgttcgagc tgctccagca gcgactgcgc atcgtcgaat ccggtgaccg
     6061 cctcgcacaa cggcgccgac aggcgttgca gcagggatgt ccgcagcagg aactcctgga
     6121 tgcgcgcggt ctgcagcgac atcacgttgt cggccaggta ctccgcgagt tcgcgcgggc
     6181 cgctgtcctc gtcgccgccc agcgacagac gcacctcggg actcagcagc gcgagacgga
     6241 acagctgcac ccctgccggc cagccttcgg tgcgccggta gatggcgtcg acctcgtctg
     6301 cgctgacacg caggttgttc gacccggcga agaacttcgc cacctcgtcg ggcgtgaagc
     6361 gcagatcatc ggctcgcacg acgatcgcca cgcggttgac cagcagcgtg gccagtccga
     6421 tctcgggcag ggaccgcgaa ccgatgaaca cgtgcacatg tggcggaagc cgggcgagca
     6481 gcgtgcggaa gaaccgcagg atcgcctcgc cgcgcagggc ctggaattca tcgaggaaga
     6541 tcgacgcctg catgtccagc cccgacagcg tgtcgagcat ccagtcggcg aggtcgcgcg
     6601 gggcgtcgcc tgcaccggtg cgtggcgctg cgctcgccgt ggcgccgtcg gtgagccgcc
     6661 ccatcattgc gaccatgtgc gattcgaacc ggcgcgggtc gttgtcggca tcgtcgaagg
     6721 tcagccaggc cgtgcgccag cctcgggccg cctgtgctga cttgatctgc tgcagcgtgg
     6781 tcgacttgcc gtgcccggcc gggccctgca gcacggtgag gcgcaggctg tcgtcgtgct
     6841 ccactcgatc gaggatgacc tgccgcggaa ccgccccggg gtagaccgga ggtgcgaaca
     6901 gcttgtgggg gcgaacctcg gaaggtgccg cgcgttcgcc catcgccgca ccggccgacg
     6961 tcacggtccg gagggtgctg cgtgagcctg gagaggggcg cggattctgg cggggcaagg
     7021 acgttcccat gaaagcgctg cgttcgagcg caggagcagg cggacaactg gcgattgttt
     7081 gttgcgccgc agcgtgacgg gttacgggct tccccgggtc ggttcggtcg aagtcggcat
     7141 gaagatattc aaaactacct gaccgggtgg tgaacccccg gccggcggtg gctctaatcc
     7201 tgacctcgac catacctaca taggagaccc tcatgtcgaa tgtccagcag gcgccgaagc
     7261 agttcgatgc cgtcgtcgtc ggtgccggtg ttgccgggct ttattcgatc tacaagctgc
     7321 gagagcaggg cttgagcgtg caggccttcg aggccggtac gggagtcggc ggcacctggt
     7381 actggaaccg ttatccgggc gcacgcgtcg actcgcaggc ctatgtctac cagtactggt
     7441 tctccgacga cctgctcaac gagtgggact ggagcgagcg tttccccgcc caggaggaaa
     7501 ccgagcgcta cctgaaccac gtcgccgaca agttcgacct gcgcagcaac atccggttca
     7561 agacccgggt gacgggcgcc agcttcgatg aatccaccca gcgctggatc gtggcgaccg
     7621 acgacggcca gacggtcagc gcgcagttcc tgatcatggg caccggcggg ctgtcggtgc
     7681 cgatgctgcc gccgttcccc ggacgcgagc gtttcaaggg ccagatcgtg cacaccgccc
     7741 agtggccgaa ggatggcgtc gatctgaagg gcaagcgcgt gggcgtgatc ggcaccggtg
     7801 ccacgggcat ccaggtgatc cagacgatcg ccgatcaggt cgggcacctg acggtgttcc
     7861 agcgcaccgc gaactacacg atcccgatgc gcaacccgaa gtacgacgac gcggatcgca
     7921 gtgagttgcg ctccagatat ccggacctga agaagcgcgt gcacggtacc ttcgcgggct
     7981 tcgactacga cttcgacagc accgacttcc tgtccctgtc tgccgaggat cgtcagaaga
     8041 agatgcaggc actctgggac gatggctcgc tcagcttctg ggtcggcagc ttcctgagcg
     8101 tgttcgtcga cgagaaggtc aatgaagagt tctcggagtt cgtccgccag cgcatccgtg
     8161 cgcgtgtcag ggacccgaag gttgcggaga tgctcgtgcc gaacgatcac ggattcggca
     8221 cacgccgggt tccgctggag acgaagtact acgaggtgtt caaccgtgac aacgtgaccc
     8281 tggtcgatac gcgggccgcg ccgatcgaat gtctcaccga gaaaggcctg cagaccgcgg
     8341 ccggcgagca cgaacttgac atcatcatct ttgccaccgg gttcgatgcc ggtaccggcg
     8401 cgctgaccaa gaccgacatc cgtgggcgca atggcctgtt gttgcgtgac gcctgggagg
     8461 ccgatggcat ccgcacgacg atgggattgc aggtccacgg ctatccgaac ctgttcatga
     8521 ccatggcgcc tttctcgccg gcggcggcct tctgcaacgt gccgacctgc gtgcagcagc
     8581 aggtggactg gatcaccgac tgcatccggt tcgtgcggga ccaggggcgc cgatccatcg
     8641 agccgagcga ggccgtggaa gcgcagtggg tcgcccacca cgatgagctg gccaacatga
     8701 tgctggtctc caagaccaac tcctggtaca tggggtccaa cgtggagggc aagcagaggc
     8761 ggctgctggc ctatcccggc ggcgtcggca attaccgcga caagtgcgat gcggtcaagg
     8821 cgagtggcta cgagggattc gtcaccgcgt gatcgggatc gatccggaaa gctgaaaggg
     8881 cggctcgaag ccgccctttt tcattctctg tgctggatcg gtgtcagccg tcccggctcg
     8941 agcggcgacg ctggacgaac ccgaggctca gcatggccgc catgatcagc gctgcgctgg
     9001 atggttctgg tacgggattg gcgaacacct ggtcctggtg actcgggctc tccagcaccc
     9061 ggacgctcac gttgttgatc gatcccaggt tggacaacca gtcgtcggcc agtgccagcg
     9121 cgccattggc cgctgtgccg ccgaagaagc gcgcggcacc cgaatcgacg tcgtagctgg
     9181 tcggactggt ttcgtaggcg agttcccaga tcgcgatctg gaacgcagcc tgctcggtgg
     9241 catcgttcac ctcatgaccc gccgagaaca gccggccgat gtcgtcatgc gcacgagtgt
     9301 tggcaaagac gtgtgccgag ccggctacct cggtgtagtc gttgtagctg ccaccgaggc
     9361 tgatgtgctg cagcaggtcg atgcagtagg tcgtgacgct cgtcgtgcca tcgagcagga
     9421 ccttgaaccc gccggcggac gtggagccat tcgcggacgg gttcggtgcc gacagctggt
     9481 agttcaccgt ctgcgacccg ttcgcgaacc cctggaattc cacgaccccg gcttgcgcct
     9541 gggccgagcc ccaagccagg caggcgagaa gaagcgggcg gatgaaggtt cgggccatga
     9601 ggatttctcg cagaagtgat gtggcttgaa gacggccgga ccgtcgatgt gccgaaggct
     9661 aaacggcgcc tgcgttggcg ctccccccgc agccaagggg tgcagcaacg aggcttcatg
     9721 ctcgatcgtg agccgcatca cgcccggtcg acctcacccc ccggttcacg gggtgagcag
     9781 cgacgacacc ggcgatgaat tcaccggcga taacatcccg ccaactcaac ccaccgggag
     9841 caatccgtat ggcaatggac gtggtcgatt acgagatcaa gggcgcggaa atgcagttcg
     9901 tcgaagtcga gctcgatccg ggcgaggcgg cggtcggcga ggccggcagc atgatgttca
     9961 tggacgccgg catctcgatg gacacggtgt tcggcgacgg ctcggccagc accggcggtc
    10021 ttttcggcaa gctgctcggc gccggcaagc gattggtgac cggcgagtcg ctgttcacca
    10081 cggtctacac caaccagggc cacagcaagc tgcgcatcgc gttcgccgca ccttacccgg
    10141 gcaagatcct gccgatggat ttgcgcaagc tgggcggcat gctgatctgc cagaaggacg
    10201 cgttcctgtg tgccgccaag ggggtgtcgc tcggcatcta tctgcagcag aagatgtcgg
    10261 tgggtttctt cggcggcgag ggcttcatca tgcagaagct cgaaggcgat ggactggctt
    10321 tcgtgcacgc gggtggcacg gttctcaagc gtgaactgca gcccggccag accttgctga
    10381 tcgacaccgg ttgcgtcgtc gcgttcacgc ctgacatcaa cttcgagatc cagtacgtcg
    10441 gcaagatcaa gaccgcgttg ttcggtggcg aaggcctgtt ctttgccaag gtgaccggtc
    10501 ccggcaccgt gtggctgcag agcctgccgt tctcgcggct ggcttcccgc gtgttcgctg
    10561 cggcgcctca gcgcggaggt tcgcgcgaag agggttcggt gctgggcggc gttctcggtg
    10621 ggggattgct cgggggctcc atcttcggcg gggatgacga atgagggccc gatcgaacga
    10681 tccggcgctt gccagatgag tcgccgtggc ccctgacgta tcctttgccg catgaaactg
    10741 ctgatcgatt cattctggcg cgcggcggcc tattgcctgc atccgcgtgt cattgcgctg
    10801 tcgcttctgc cgctggtgct gatggtcgcg atcggtctcg gtgtcggcta cttcttctgg
    10861 gattcggtcg tcgcctgggt ggcctccatt ctcgag
//
LOCUS       DAOORV010000118        10842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7585_length_10842_cov_6.394642, whole genome shotgun sequence.
ACCESSION   DAOORV010000118 DAOORV010000000
VERSION     DAOORV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7585_length_10842_cov_6.394642"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKV56_13795"
     CDS             complement(<1..430)
                     /locus_tag="PKV56_13795"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOX68932.1"
                     /translation="MPANASPGTAVEPREVREWLMRIHNAASQRNFQGTFVVSSPGVV
                     SSARISHFHEGADQFERIEPLEGQARQVLRHNGIVHTVWPGEKVALIEQREVLNSFPS
                     LLLDGDDRITDVYEVQAQKGERVAGHDANVLTLKPRDEYRY"
     gene            complement(513..1190)
                     /locus_tag="PKV56_13800"
     CDS             complement(513..1190)
                     /locus_tag="PKV56_13800"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HOX68933.1"
                     /translation="MTNEPDSSDDAATARRQDMSALVDGELAAEGVQRLCAAWRSEPS
                     SREAWHAYSLIGDVMRSEDLASAAAHDERFLRDFRQRLSDEPVVLAPQPASAPRYASA
                     PAATRRSGIRSWRAPAAVAAGLMVVVGAMVTTQAPDQPTVDSASMAAVPAGRASGLVP
                     APQLAGLTPQAEVTPATTELVFDGQVIRDPRLDRYLLAHKQFSGSSVLGAPSGFLRNA
                     VAEVPAR"
     gene            complement(1263..1871)
                     /gene="rpoE"
                     /locus_tag="PKV56_13805"
     CDS             complement(1263..1871)
                     /gene="rpoE"
                     /locus_tag="PKV56_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOX68934.1"
                     /translation="MNAPDADAPLIERVKQGDVRAFEMLVVKYQRRIERLIGRMVRDV
                     DLVPDIAQETFIRAYRAIPQFRGESAFYTWLYRIAVNTAKKSLGELKRDLLVTESARA
                     SRDDEDETSRTENELTDGETPESLLASKEVAMTVNAAIEALSEDLRQAITLREIEGLS
                     YEEIAELMNCPIGTVRSRIFRAREAIALRLRPLLDTRLGERW"
     gene            complement(1876..2367)
                     /locus_tag="PKV56_13810"
     CDS             complement(1876..2367)
                     /locus_tag="PKV56_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68935.1"
                     /translation="MQAAPPVQVLVQSFGAWNGLLISLSIAALGVSGGWFIGHGDVLP
                     AWVAVVMVAALSFALVGAWTSRRSHPFRLRWDSQRWYLTEGQIPADEAGPLHVQVMLD
                     AGNWLLLRIVPEDRSSPLAARWLPVQRRGIPDQWHALRCAVYCPRLKLTRPSSLDGPI
                     RIE"
     gene            complement(2389..3633)
                     /gene="fabF"
                     /locus_tag="PKV56_13815"
     CDS             complement(2389..3633)
                     /gene="fabF"
                     /locus_tag="PKV56_13815"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOX68936.1"
                     /translation="MSRRRVVVTGLGMISPVGNSVVDAWSNVLAGRSGIADITRFDAS
                     GFACRFAGEVKNFNVEDFFPAKEARHMDVFMQYGMAASIQAVRDSGLKTNDELSEEEA
                     ERIGCIVGSGIGGLPLIEDTHAELTARGPRRISPFFVPASIINMISGHLSIKFGFQGP
                     NLAIVTACTTGLHCIGQAGRMIECGDADVMVAGGAEATVSPLGVGGFAAARALSTRND
                     DPATASRPWDRDRDGFVLGEGAGAMVLEEYEHAKRRGANIYAELIGFGMGADAHHMTA
                     PSVDGPKRSMRAALRNAGVNAEQVQYLNAHGTSTPLGDVNETNAIKLAFGDHAHKLVV
                     SSTKSMTGHLLGGAGGVESVFTVLAVRNQVAPPTINIFNQDPDCDLDYCANTARDMKI
                     DLAVKNNFGFGGTNGTLVFKRV"
     gene            complement(3728..3967)
                     /gene="acpP"
                     /locus_tag="PKV56_13820"
     CDS             complement(3728..3967)
                     /gene="acpP"
                     /locus_tag="PKV56_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX68937.1"
                     /translation="MSDIESRVKKIIAEQLGVPESDVSNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEEAEKITTVQLAIDYALKHQKA"
     gene            complement(4096..4845)
                     /gene="fabG"
                     /locus_tag="PKV56_13825"
     CDS             complement(4096..4845)
                     /gene="fabG"
                     /locus_tag="PKV56_13825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOX68938.1"
                     /translation="MTAEAVTPQVALVTGASRGIGRAIALELARRGLIVIGTATTDSG
                     AQAIAAALAGYSDCRGVCLDVNDSAGIDATLDTILKAHGALHVLVNNAGITRDNLSMR
                     MKDADWDAVIDTNLSAVFRLCRAVIRPMVKQRGGRIINITSVVGASGNAGQANYAAAK
                     AGVAGMTRSLARELGSRGITVNCVAPGFIATDMTDALSASQKEALLTQIPLGRLGRVD
                     DVAQAVAFLASDAAAYITGTELHVNGGMFMT"
     gene            complement(4842..5780)
                     /gene="fabD"
                     /locus_tag="PKV56_13830"
     CDS             complement(4842..5780)
                     /gene="fabD"
                     /locus_tag="PKV56_13830"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOX68939.1"
                     /translation="MTSFAFLFPGQGSQAVGMLDAWGDHPAVIQTLTEASDALGIDVG
                     RLVREGPKEQLDLTTNTQPVMLTAGIACYRAWIAEAGRVPDALAGHSLGEYSALVAAG
                     ALTLTDALPLVRFRAQAMQEAVPVGAGAMAAILGLEAQVVRDGCAEVAQASGEVVEAA
                     NFNDPKQTVISGSQAGVAKACELLKAAGAKRTLLLPVSAPFHCSLMKPAAERLKSKLA
                     SVSLNAPAVPVVNNIDVRVESDPGRIRDALYRQAFGPVRWFETVQALRALGIGLLVEC
                     GPGKVLAGMVKRIDSTAESGAVFDPASLAEVRGLLA"
     gene            complement(5823..6833)
                     /locus_tag="PKV56_13835"
     CDS             complement(5823..6833)
                     /locus_tag="PKV56_13835"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOX68940.1"
                     /translation="MNGPASNAVIPTGRYSRIAGTGGYLPAQRLTNAALVDRLASRGV
                     ETSDDWIVERTGIRARHFAAEGESCSDLALAAARRAIEAAGCDAASIDLIIVATSTPD
                     MVFPSTACIVQQKLGVSGCPAFDVQAVCSGFVYGLTLADSMIRVGAAHRALVIGAEVF
                     SRILDFNDRTTCVLFGDGAGAVILEASDTPGIIASELHADGRFVDILCTPGSVSGGQV
                     RGDPTLKMDGQAVFKLAVGVLDEVARAVLIKAGRTEADIDWLIPHQANIRIMQSTARK
                     LKLSMDKVVVTVADHGNTSAASIPLALDVAVRSGRIARGDTVMLEGVGGGFTWGAVLL
                     DF"
     gene            complement(6830..7939)
                     /gene="plsX"
                     /locus_tag="PKV56_13840"
     CDS             complement(6830..7939)
                     /gene="plsX"
                     /locus_tag="PKV56_13840"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOX68941.1"
                     /translation="MTHDVLRRPIRLAIDCMGGDHGPSVVLPACESFLAHQPRAELLL
                     VGRPEALDAARAWPRCTFIEATEVVEMDDAIEVALRRKKQSSMRLAVNLVKPDEFGVS
                     AADACVSAGNTGALMAVSRYVLKTLEGIDRPAIASVMPNQKDGYTTVLDLGANVDCTA
                     EHLLQFAVMGSALVAAVEGKEQPSVGLLNIGAEAIKGSDTIKQAGELLRAAAASGHLN
                     FHGNVEGNDIFRGTADIVVCDGFVGNVALKTAEGLASMLSSFIKQEFTRNAFTRMAAL
                     VAMPVLNRFKARVDHRHYNGAALLGLRGLVFKSHGSADAFAFEQALNRAYDAARNGLL
                     DKVHDRIRDTLQAMPVAATPGENENTKMIASSQLA"
     gene            complement(8114..8296)
                     /gene="rpmF"
                     /locus_tag="PKV56_13845"
     CDS             complement(8114..8296)
                     /gene="rpmF"
                     /locus_tag="PKV56_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOX68942.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALNTPGTAIEPTTGESHLRHHISP
                     NGFYRGRKVLKTKADA"
     gene            complement(8444..8995)
                     /locus_tag="PKV56_13850"
     CDS             complement(8444..8995)
                     /locus_tag="PKV56_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOX68943.1"
                     /translation="MEIRQFDPRSVDVAAFAKQGGDESGSCALVLLDRLCDEAHSDAK
                     PVADDLMHWRATGEMRSQRGAEPQVWLHLEGRARLALVCQRCLQSVETQLHARRSFQF
                     VAGEDQAAAIDADSEDDVLALTRALDLIGLIEDELLLALPLVPRHDVCPQPLKVQDDA
                     DPFEERAHPFAALGSLKRKLPPS"
     gene            9062..9676
                     /locus_tag="PKV56_13855"
     CDS             9062..9676
                     /locus_tag="PKV56_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOX68944.1"
                     /translation="MHTTPPLILGSTSIYRRKLLARLRIPFQVVAPNVDEAARVAELP
                     EHLATRLAMTKARAVAQLHPDSVVIGSDQVAELDGVAVGKPGSHRYATEQLRAMSGRQ
                     VIFHTAVAVVCSARGFEESDLARVSVKFRSLTHDEIERYLQAEQPYDCAGSAKSEALG
                     IALLQAVESDDPTALIGLPLIRTCSLLRRAGLDPLGTVDRAVGR"
     gene            9673..10446
                     /locus_tag="PKV56_13860"
     CDS             9673..10446
                     /locus_tag="PKV56_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX68945.1"
                     /translation="MSGKLYLVPNTLDFGVAAHAEGASGQPDLQEVLPLEVLRIAARL
                     PCWVAENAKTTRAFLKRVNEVVPLSQALQAISIVELPRSNKGAPQTPAANLGALLAPA
                     IAGQDMGLISEAGLPAVADPGAALVQAAHVLNVRVEALSGPSSLMLALAASGLNGQSF
                     AFVGYLPVDSAARLTRIRELEALSRKTQQTQLMIETPYRNAALLAALLSALSGATRLS
                     VSCGLTLEQGWSRTDTVSGWKARSIEIPSDTPAVFSLLA"
     gene            complement(10474..>10842)
                     /locus_tag="PKV56_13865"
     CDS             complement(10474..>10842)
                     /locus_tag="PKV56_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOX68946.1"
                     /translation="EKLIKKKIEIEPIELDDERPRREHRPERVRSEVEDEVPEVASAA
                     PRTALPRAPQRDRGEPGRKPSMDPFFDRPYEPSATATPAWEAAAPAVSLSSRATSPNI
                     KPKRRVAALFGEKVSSDQDE"
BASE COUNT         2029 a         3548 c         3372 g         1893 t
ORIGIN      
        1 cgtagcggta ctcgtcacga ggcttcagcg tcagcacatt cgcatcgtgg cctgccacac
       61 gctcgccctt ctgggcttgt acttcataga catcggtgat ccggtcgtca ccatccagca
      121 acagcgaagg gaacgagttg agcacctctc gctgttcgat cagggcgacc ttctcgccgg
      181 gccagacggt gtgcacgatt ccgttgtggc ggagcacctg ccgagcctgc ccctccagag
      241 gctcgatgcg ctcgaactgg tcggcgcctt catgaaaatg ggaaatgcgc gcgctggaga
      301 ccactccagg agaactgacc acgaacgtcc cctgaaaatt ccgttgactg gccgcgttgt
      361 gtatccgcat caaccattca cgaacctcgc gcggctcgac ggcggtcccc ggagatgcgt
      421 tggcgggcaa ctcacaggcg agcaccagcc agacacccca gaagaccgag ccccaggaac
      481 tgcctcggcg agaggaatgc gacttcaaca cgtcagcggg caggaacttc agcaacggcg
      541 ttgcgaagga aacccgaagg cgctccgagt accgagcttc cggagaactg cttgtgagca
      601 agcaggtatc gatccagtcg agggtcccgt atcacctgcc cgtcgaagac cagttcggta
      661 gtcgctggag tcacttcagc ttgaggagtc aaccccgcca actggggcgc cgggaccagg
      721 cctgatgcac ggccggccgg taccgcagcc attgaagcgg agtcgaccgt cggctggtca
      781 ggagcctggg tggtcaccat cgcgccgacc acgaccatca gacctgcagc cacggctgcc
      841 ggcgcacgcc aggaacgtat gccggatcgt cgcgtggcag caggggcact tgcatatcgg
      901 ggtgcgctgg ccggttgggg cgcgagcacc acaggctcgt ctgacaggcg ctggcggaaa
      961 tcgcgcagaa aacgctcatc gtgcgccgca gcggaggcca gatcctccga acgcatgacg
     1021 tcgccgatga ggctgtaggc atgccacgcc tcgcgggagg acggttcgct gcgccacgca
     1081 gcgcaaagcc gttgcacgcc ttcggcggcg agttcgccgt cgacaagtgc agacatgtcc
     1141 tgccgccgag cagttgccgc atcgtcgctg gaatccggct cgtttgtcat gtcaatctcc
     1201 catttcaccc atgttcacgc cccacgctcc actggcagca cgccactcgc cagttcccgc
     1261 ggtcaccatc gctcgcccag gcgagtgtca agcagcggcc tcagacgcaa cgcgatggct
     1321 tcccgcgccc ggaagatgcg ggaacgcacc gtgccgatgg gacaattcat caattcagca
     1381 atctcttcgt agctcagtcc ttcgatctca cgcagcgtga tcgcctgccg gaggtcctcc
     1441 gacagggctt cgatggcagc gttgaccgtc atcgccactt ccttgctcgc caagagcgac
     1501 tcgggcgtct cgccgtcggt tagttcgttc tcggtacggg aagtttcatc ttcgtcgtcg
     1561 cgggaggcgc gcgccgactc ggtcaccagc aaatcccgct tgagttcgcc cagagatttc
     1621 ttggccgtat tgaccgcgat gcggtagagc caggtgtaga aggcgctttc accccggaac
     1681 tgcgggatgg ctcggtacgc gcgaatgaag gtttcctgtg cgatgtcggg cacgaggtcc
     1741 acgtccctga ccatgcgtcc aatgagccgc tcgattcgcc gctggtactt gaccacgagc
     1801 atttcgaacg ctctgacatc gccctgcttg acgcgttcta tcagcggcgc gtcggcatcg
     1861 ggggcattca ttcggtcatt caatgcggat ggggccgtcg aggctgctgg gacgggtcaa
     1921 tttgaggcgc gggcaatata ccgcacagcg cagagcgtgc cactgatctg ggatgcctct
     1981 ccgctgcacc ggtagccaac gagccgccag cgggctggag cgatcctccg gcacgatcct
     2041 cagcagcagc cagttccctg cgtcgagcat cacctgcaca tgcaacggac cggcttcatc
     2101 cgctggtatc tgaccttcgg tgagatacca gcgctgcgaa tcccagcgca atcggaatgg
     2161 atgactccga cgggatgtcc atgccccgac cagcgcgaaa gacaatgccg caaccatcac
     2221 caccgcaacc caggcaggca gaacatcgcc gtgcccgata aaccagccac cggaaacccc
     2281 cagggctgcg atgctcaagg aaatcaacaa gccgttccag gcgccaaagc tctgaacaag
     2341 cacctgaacc ggtggcgccg cttgcatacc gggcgccacg gctcgtcttc agacacgctt
     2401 gaagacaagc gtgccgttcg tcccgccgaa accgaagttg ttcttgactg ccagatcgat
     2461 cttcatgtcc cgcgccgtgt ttgcgcaata gtcaagatca cagtcgggat cctggttgaa
     2521 gatattgatg gtcgggggcg ctacctgatt gcgaaccgcc agaacagtga aaaccgactc
     2581 cacgccaccg gccccaccga gcaggtgtcc ggtcatcgat ttggtcgaac tgaccaccag
     2641 cttgtgggca tggtcgccga aggccagctt gatggcgttg gtttcgttca catcgcccag
     2701 cggcgttgaa gtgccatggg cgttgaggta ctggacctgc tcggcattga cgccggcatt
     2761 gcgcagagct gcacgcatcg agcgcttcgg gccatccacg ctcggggccg tcatgtggtg
     2821 cgcgtcggcg cccatgccga aaccaatcaa ttcggcatag atgttggcgc cgcgacgctt
     2881 ggcgtgctcg tactcctcga gcaccatggc gcccgcacct tcgccgagca cgaagccatc
     2941 gcggtcacga tcccagggtc gcgatgcggt agccgggtcg tcattgcggg tcgacagagc
     3001 tcgggcggcg gcaaatccgc cgacacccag cggcgacacg gtcgcctcgg cgccaccagc
     3061 caccatcaca tcggcatcac cgcactcgat catcctgcca gcctgcccaa tgcaatgcag
     3121 acccgtagtg cacgccgtca cgatcgccag gttgggtccc tggaaaccga acttgatcga
     3181 caggtggccc gagatcatgt tgatgatcga agcgggcaca aagaagggag agatgcggcg
     3241 tgggccgcgg gccgtgagtt cggcgtgcgt gtcctcgatc agcggcaagc cgccgatgcc
     3301 cgacccgacg atgcaaccga ttcgctcggc ctcctcctcg ctcaattcgt cattggtctt
     3361 caggcctgaa tcgcgcacgg cctgaatgga cgctgccatg ccgtactgca tgaacacatc
     3421 catgtgccgg gcttccttgg ccgggaagaa gtcctcgacg ttgaaattct tcacttcacc
     3481 ggcgaagcgg caggcgaatc ccgatgcgtc aaaccgggtt atgtcggcaa tgcccgaccg
     3541 accggccagc acgttggacc aggcatcgac gaccgagttg cccacggggc tgatcatccc
     3601 caggcctgtg accaccacac gccgacggct catcggaccc cctgggatgg gacagcccgg
     3661 tctgtcgata cacaggctgt ctcgatcgaa agatgctgac ggaaatgcat gcgacgaagg
     3721 ctgagcttca agccttctgg tgcttcagcg cgtaatcgat cgcgagctgc acggtggtga
     3781 tcttctcggc ctcttcgtca ggaatctcga tgccgaactc gtcttcgagg gccatcacca
     3841 gttccaccgt gtcgagcgaa tcggcgccca gatcagcaac aaaggccttc tcgttggaca
     3901 cgtcagattc gggcacgccc aattgttcgg cgatgatttt cttgacacgg gattcgatat
     3961 cgctcatgac tcctccagga gtgttgtctt aaacagcccg ggattctaaa ggttgcaccg
     4021 cttcgttcca ggcgatacgc gcttcagccg gccgggcagg tccgggttga caaaagtggg
     4081 gttggcagga cgggatcagg tcatgaacat tccgccgttg acgtgcaact cggtgccggt
     4141 gatgtaggcc gctgcatcgg aggccaggaa ggcgaccgcc tgtgccacat catcgactcg
     4201 gccgagccgg cccagcggaa tctgcgtgag cagggcctct ttctgagacg ctgacaatgc
     4261 atcggtcatg tcggtggcga tgaaacccgg ggcgacgcag ttgacggtga tgccccggct
     4321 tcccagttca cgcgcaagtg agcgagtcat gccagccacg cccgccttgg ccgctgcgta
     4381 attggcctga ccggcattgc cgctcgcgcc gacgaccgag gtgatgttga tgatgcggcc
     4441 accgcgctgc ttcaccatgg ggcgaatcac cgcacggcac aaccggaaca cggcgctcag
     4501 gttggtgtcg atcacagcgt cccaatcggc atccttcatg cgcatcgaca ggttgtcgcg
     4561 ggtgatgccg gcgttgttca ccagcacatg cagggcgccg tgcgccttga ggatggtgtc
     4621 gagcgtcgca tcgatgcctg cgctgtcgtt gacatcgaga cagacgccac ggcagtcgga
     4681 gtagcccgcc aacgccgctg cgatggcctg agcgcccgag tcggtagttg ccgtgccgat
     4741 cacgatcaag ccgcgccgcg ccagttccag cgcgatggca cggccgatgc cacgcgacgc
     4801 gcctgtgacc agcgcgacct gtggcgtcac tgcttctgcc gtcatgccag caacccccgc
     4861 acttctgcca gagaagccgg gtcgaagacg gcgcctgact cggcggtgga atcgatgcgc
     4921 ttgaccatgc cggcgagcac cttgcccggc ccgcattcga ccagcagacc gatgcccagc
     4981 gcacgaaggg cctgcacggt ctcgaaccag cgcaccggcc cgaaagcctg ccggtacagc
     5041 gcatcacgaa tcctgccggg gtcggattcg acgcgaacgt cgatgttgtt caccaccggc
     5101 acggcgggag cgttgaggct caccgaagcc agtttgctct tcaggcgctc ggcagccggc
     5161 ttcatcaggc tgcaatgaaa cggtgcggag acgggcaaca gcagggtccg cttggctccc
     5221 gccgccttca gcaactcgca ggccttcgcg acgcccgcct gggagccgga gatcacggtc
     5281 tgtttcggat cattgaaatt ggctgcttcg accacctcac cgctcgcctg cgcaacttcg
     5341 gcacagccgt cgcgaacgac ctgcgcctca agcccgagga tcgccgccat cgcgccggcg
     5401 ccgacgggca cagcctcctg cattgcctgc gcacgaaagc gcaccagcgg cagggcatcg
     5461 gtcagggtca acgcaccggc cgccaccagc gcgctgtact caccgagcga atgcccggcc
     5521 aaggcatcag ggacacggcc ggcttctgcg atccaggcgc gatagcaggc aattcccgca
     5581 gtgagcatga cgggctgcgt gttggttgtc aggtccagct gttccttggg cccctcgcgc
     5641 accaggcgac cgacgtcgat gcccagggca tccgatgcct cggtcagtgt ctggatcacg
     5701 gccggatggt cgccccaggc gtcgagcatg cccacggcct gggaaccctg gccagggaaa
     5761 agaaaggcaa acgaagtcat cgagattgaa aggggtcagt gttgcagcga tgagccgcag
     5821 gttcagaaat cgagcaggac ggcaccccac gtgaagccgc caccgacgcc ttcgagcatc
     5881 acggtatctc cgcgggcgat gcgtccgctg cgcaccgcga catccagcgc aagagggatc
     5941 gatgctgcag acgtgttgcc atgatcggcc acggtgacaa ccaccttgtc catcgacagc
     6001 ttgagcttgc gcgccgtgct ttgcatgatg cggatgttgg cttggtgagg aatcaaccag
     6061 tcgatgtcag cctcggtgcg tccggccttg atcaggaccg cacgcgccac ctcgtcgagc
     6121 actccgacgg cgagcttgaa caccgcttgc ccgtccatct tcaatgtagg gtcgcctcga
     6181 acctgcccac cggaaacgct gcctggcgta cagaggatgt cgacgaaccg gccatccgcg
     6241 tgcagttcgc tggcaatgat gccgggcgtg tcactggcct ccaggatgac tgcgccagcg
     6301 ccgtcaccga acagcacgca agtggttcga tcgttgaaat caagaatgcg ggaaaacact
     6361 tcagccccga tcaccagcgc cctgtgggcc gcgccgaccc ggatcatcga gtcggcgagc
     6421 gtcagtccgt agacgaagcc ggagcacacc gcctgcacat cgaaagcagg acagccggac
     6481 acaccgagct tctgctgcac gatgcaggcc gtggacggaa aaaccatgtc aggcgtcgag
     6541 gtggcgacga tgatcaggtc gatcgatgcg gcatcgcagc cggcagcttc gatcgcacgc
     6601 ctcgccgcag ccagcgccag atcgctgcac gactcaccct cggccgcgaa atgccgcgct
     6661 cggatcccgg tgcgctcgac gatccagtca tctgacgtct cgacaccgcg ggaagccagt
     6721 cgatccacca gcgcggcatt cgtgagtcgc tgggcgggca gatatccgcc ggttccggca
     6781 atacgtgaat aacggccagt cggaatgact gcgttcgaag cggggccgtt catgcgagtt
     6841 ggctggacgc aatcatcttc gtgttttcgt tctcaccggg ggtcgctgca accggcatgg
     6901 cctgcaaggt atcgcggatg cggtcgtgga ccttgtccag caaaccgttg cgggccgcat
     6961 cataagcccg gttcagcgcc tgctcgaacg caaacgcatc ggccgatccg tggctcttga
     7021 acaccaagcc gcgaagcccg agcagagccg cgccgttgta gtgcctgtga tcgacccggg
     7081 ccttgaagcg gttcaagacc ggcatcgcaa ccagcgccgc catccgggtg aaggcgttgc
     7141 gcgtgaactc ctgcttgatg aagctcgaga gcatcgatgc cagcccttcc gcggtcttca
     7201 acgccacatt gccgacgaat ccgtcgcaga cgacgatatc ggccgtacca cggaagatgt
     7261 cgttgccttc gacattgccg tggaaattga ggtgcccgct ggccgcagcg gcgcgcagca
     7321 actcgcccgc ctgcttgatg gtgtcgctgc ccttgatcgc ttcggcgccg atattcagca
     7381 agccgacgct gggctgctcc ttgccttcga cggcggccac caatgcactt cccatcaccg
     7441 caaattgcag gaggtgctca gcagtgcagt cgacattggc gcccagatcg agcaccgtgg
     7501 tgtagccatc cttctggttc ggcatgacgc tggcaatggc cgggcgatcg atgccttcaa
     7561 gcgtcttcag cacataacgg gacaccgcca tcagcgcccc cgtattgccg gcggatacgc
     7621 acgcatccgc ggccgacacg ccaaactcgt caggcttcac gaggttgacg gccagtcgca
     7681 tcgacgactg cttcttgcgc cgcaaggcca cctctattgc atcgtccatc tcgacgactt
     7741 cggtggcctc gataaaggtg cagcgcggcc atgcgcgcgc agcatcgagc gcttcgggcc
     7801 gtccgaccag cagcagttcc gcacgaggct gatgcgccag aaaggattcg caggccggca
     7861 gcaccaccga tgggccgtga tctccgccca tgcagtcgat ggccaggcgt atcggtctgc
     7921 gcaggacgtc gtgggtcatg gtctgggggc cttgctcagg ccagcgcagg cgttcggctc
     7981 gcccaccgga ccggaacaga aatgcaaaag cccggcatcg ctgatttgca ggggccgggc
     8041 catcggtcgc aacggatcat cgccgggtgc gcggccgtgc ggctgcgctg gcgatcatct
     8101 aggcgtcaaa cgctcacgcg tcggctttgg tcttgagaac cttgcgccca cggtaaaagc
     8161 cgttggggct gatgtggtgg cgcagatgcg actcaccggt ggtcggctcg atggcagttc
     8221 ccggcgtgtt cagcgcgttg tgtgaacggt gcatgccgcg cttggaaggc gacttcttgt
     8281 tttgctgaac agccatttca ttctccgcaa tcgttggtgg tggcgtccgg tggcgaacca
     8341 ctccgggaca gacctctcaa agagggtccg caggcgcttg atccggacga atccacgaga
     8401 acaacgcctg ctgaaaagcc cacgagtata tcaggatgga aggtcagctg gggggcagct
     8461 ttcgcttgag gctgcccagt gcggcgaacg ggtgggcgcg ctcctcgaac ggatcggcat
     8521 catcttgcac cttcagcggc tgtggacaaa catcgtggcg cggcaccagg ggaagtgcaa
     8581 gcaacagctc gtcctcgatc aatccgatca ggtcgagcgc ccgtgtgagc gccaacacat
     8641 cgtcctcgct atccgcatcg atcgcagcgg cctgatcctc gccggcgaca aactggaaac
     8701 ttcgacgcgc atgcaattgc gtttccaccg actgcaggca gcgctgacaa accagggcca
     8761 gccgagcccg accctcgaga tggagccaga cctgaggctc ggcgccgcgc tgtgatcgca
     8821 tttctcccgt ggctcgccag tgcatcagat catccgcgac gggcttcgca tcggaatggg
     8881 cctcgtcgca caggcgatcc agcaggacca gggcgcagga cccggactcg tcaccgccct
     8941 gcttcgcaaa ggcagcgaca tccacgctgc gcggatcgaa ctgacggatc tccatgcggc
     9001 gattttaggt tgccgaccgc tgcggcgccg gatgcaacgg ccgatgggac aatgcgcgcc
     9061 gatgcacacg acaccaccgc tgatcctcgg atcgacctcg atctatcgtc ggaagttgct
     9121 ggcccgcttg cggattccgt tccaggtggt cgcaccgaac gtggacgaag cggcacgcgt
     9181 cgcggagctc cccgaacacc tggcaactcg cctggcgatg accaaggccc gcgcggtggc
     9241 ccagctgcat cccgattccg tggtgatcgg atcggatcag gtcgcagagc tcgacggcgt
     9301 ggcggttggc aagcccggaa gtcaccggta cgccacggag caactgcgcg cgatgagcgg
     9361 acgccaggtc atcttccata ccgcggtggc ggtcgtctgt tccgctcgag gtttcgagga
     9421 gagcgacctc gccagggtga gcgtgaaatt ccgatcgtta acccatgacg agatcgagcg
     9481 ctacctgcag gccgaacagc cttacgactg cgccggcagc gccaaatcgg aggcactcgg
     9541 catcgcactg ctgcaggcgg tcgagtccga cgaccccacc gccctgatcg ggctgccgct
     9601 gatccgcacc tgctcgctgc tgcgacgcgc cgggctcgac cccctgggca ctgtcgatcg
     9661 cgcggttggc agatgagcgg gaagctgtat ctggtgccga acaccctgga tttcggcgtc
     9721 gccgcccacg cagagggggc atccggccag ccagacctgc aagaggtcct gccgctggaa
     9781 gtgctacgca tcgccgcccg cctcccctgc tgggtggcgg agaacgccaa gaccacccga
     9841 gcgttcctga agcgtgtgaa tgaagtggta ccgctttcgc aggcactgca ggcgatctcc
     9901 atcgtcgaac tgccccgctc gaacaagggg gcgccccaga cccccgctgc aaacctcggc
     9961 gccttgctgg cgcccgcaat cgcaggtcag gacatgggtt tgatctccga ggcgggcctg
    10021 ccagcggtcg ccgatccagg tgcagccctg gtgcaggcag ctcatgtgct gaacgtgcgc
    10081 gtcgaagcgc tttccggtcc gagctcattg atgctggcgc tggccgccag cggcctcaac
    10141 gggcagagct tcgccttcgt cggctacctt cctgtcgatt cggccgcgcg tctcacgcga
    10201 atccgtgaac tggaggcgct gtcacgcaag acgcagcaga cccagctgat gatcgaaacg
    10261 ccctaccgca atgccgcgct gctggcagca ctgctgagcg cgctgtcggg cgccacacgc
    10321 ctgtcggtga gttgcggcct gaccctggaa caggggtgga gtcgcaccga caccgtctcg
    10381 ggctggaagg cacggagcat cgagatcccc tctgacacgc ccgccgtttt ttccctgctc
    10441 gcctgaaccg aagaggtcga caagcactga cgctcactcg tcctgatcgg aagacacctt
    10501 ttcgccgaac agggccgcga cgcgacgctt gggcttgatg ttgggagagg tcgcacgcga
    10561 cgacagactg accgcaggtg cagcagcctc ccacgctgga gtggccgtgg cgctgggttc
    10621 gtagggccgg tcgaaaaacg gatccatgga aggtttgcga ccgggctcgc cgcgatcgcg
    10681 ctgcggcgca cgcggcaagg cagtgcgggg cgcagcagac gcaacctcgg gcacttcatc
    10741 ctccacctcg gagcgcacgc gctcgggccg gtgctcccgg cgggggcgct cgtcgtcgag
    10801 ttcgatgggc tcaatctcga tcttcttctt aatcagcttc tc
//
LOCUS       DAOORV010000119        10700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_7701_length_10700_cov_7.913668, whole genome shotgun sequence.
ACCESSION   DAOORV010000119 DAOORV010000000
VERSION     DAOORV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_10700_cov_7.913668"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKV56_13870"
     CDS             <1..220
                     /locus_tag="PKV56_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOX68947.1"
                     /translation="HTVGEFGVVLMIGGNIPGQTRVLSVAIYDHVEAAEFDQAHRLAA
                     GMVAFALIVLVTLYITNRPPRDDAEIAR"
     gene            217..1296
                     /gene="modC"
                     /locus_tag="PKV56_13875"
     CDS             217..1296
                     /gene="modC"
                     /locus_tag="PKV56_13875"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HOX68948.1"
                     /translation="MIEAHLRLQRRDFRLEVALALPSRGVTALFGPSGCGKTTVLRAL
                     AGLERASGRIALDDEVWQDDAIGCFVPTHRRPLGYVIQEAALFPHLDVRGNLDYGRSR
                     AVSESSRVPLDAIIELLGIGHLQARRAATLSGGERQRVAIARALATGARLLLMDEPLA
                     ALDAARKAEILPYLERLHRELAMPVVYVTHSMDEVARLADHLVLMREGRVVAAGALAD
                     LMARTDLPLSRLDDAGVVIVAEVAEHDELHGMTRIAFDGTSMWVGQTAAQPGEQVRAR
                     VLARDVSVTRQRPTETSILNVLPVRLDSIERDQSTALLRLQAGAVTGTGGWTLLARIT
                     TRSLDALELRPGDELHAQIKGVALM"
     gene            1306..1671
                     /locus_tag="PKV56_13880"
     CDS             1306..1671
                     /locus_tag="PKV56_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68949.1"
                     /translation="MERIADHWQAHLPDDLHEHVVWPVRFEAHHDDGVPASKHRGYDA
                     LGLLCWYRHHFSQWDASFDDNDQPTAQLLREEDFEAWRTLAGPWIRRVQRLDGDGHPC
                     GSLSDSGFEVVEPAAIPRL"
     gene            1704..2552
                     /gene="modD"
                     /locus_tag="PKV56_13885"
     CDS             1704..2552
                     /gene="modD"
                     /locus_tag="PKV56_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HOX68950.1"
                     /translation="MERIDDDELRRWLREDAPHGDLTTRALHIGDRAGQLRFETRASM
                     RVAVVEEAARLFELCGCDAIVVRGSGSEAQPGDPLLTATGPASGLLLAWKVAQTAVET
                     ASGIASEAARIIALLRDAGFGQPLACTRKNWPGGRALAARGVWAGGAVMHRLGLSETL
                     LVFPEHRSFLAPTEIVSSMKALRSAQPEKKLVVEVGSVDEAMCFAQAGAEVLQLERFS
                     PAALTALRQRLDAAALRPLLAPAGGVTAANALDYARAGADFLVSSAPYFAPPADVKVV
                     IGPSRL"
     gene            2623..2904
                     /locus_tag="PKV56_13890"
     CDS             2623..2904
                     /locus_tag="PKV56_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HOX68951.1"
                     /translation="MKSSTIPSVRVEPEFRAEVEAVLSTGETLSEFVESSVRASVERR
                     RAQAEFIARGLQSRDQARQTGDYVDADVVLEGLQRKLDAVHARLAKTRK"
     gene            2901..3218
                     /locus_tag="PKV56_13895"
     CDS             2901..3218
                     /locus_tag="PKV56_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOX68952.1"
                     /translation="MSFRVRLTRDAAADLDRLFDFVIERELAREGGDLTMAEQAIAAL
                     RAGIATLKSSPFTCRKAGQSPFLRELVIPFGRSGYVALFEIEDASNVVVVAVRHQRED
                     DYH"
     gene            complement(3289..4176)
                     /locus_tag="PKV56_13900"
     CDS             complement(3289..4176)
                     /locus_tag="PKV56_13900"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOX68953.1"
                     /translation="MSVVDIAWRAHPYFPLVLLFNRTEARSRATEPAAWWTEPAGVLA
                     GRDLAGQGTWLAVSREGGRFALVTGYREASPPPAGAPSRGGLPLDYLASGEDPVVFAR
                     RFMRDKGPYAPFNLIVGNPRQAHYAATRARLPIALTEGLHTLSNGLLDQRWPQTERLG
                     TLFGAYIKAHGGFTTLLDGYPKLRDAQALCGFELPVPDDVLCASDIAEAAFVMLADRH
                     TSAAGLPETGLDPEEEQRRSAVFIAGGDTGTRSSTVFIMGRDGSLHFEERSFDADGAL
                     CGRVLEQWQQDPAVFGAPA"
     gene            complement(4187..4978)
                     /locus_tag="PKV56_13905"
     CDS             complement(4187..4978)
                     /locus_tag="PKV56_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX68954.1"
                     /translation="MDGIPALASYHEWLAVERQLKAGCALADRMGLRAGQRVLELGCG
                     TGLLAEHLVERVGSQGEVLGLDAMALRVQIAHQRGRYNLRFQVGDPCSLQRFGAGSFD
                     AIVANGVLHTWPDAAAALTECARLLAPAGCLGLATQSAAHPHPAERLQRELMAEPPYA
                     DDPPPAESRRYPVGADELDRLLRGAGLVSVQVEARPETFLHATGEGALEHMQASAWGH
                     FLMHLPPDLRDRARAEILRRLEALRGPDGIRHDAMQIVAIAFKSP"
     gene            complement(4984..5673)
                     /locus_tag="PKV56_13910"
     CDS             complement(4984..5673)
                     /locus_tag="PKV56_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HOX68955.1"
                     /translation="MQALNQISPITVVGRWQNPAGVACTSHGAMPKGLRLTIWLDLMS
                     CATAADAPEVQAMAGVLATAFEVHGFDQLPISGLSDSATRRMMARWFPGADRLLALDW
                     QELRHATRDEPRYDEIEDLVGLLTTGVCPTHAGREEVIWVAHALSQASLGDNHLWQDL
                     HLPSRRELSALMSGWFPAIAARNDRDMKWKKFLYKQLCERAELTICRAPSCSVCTDYA
                     HCFGPEDAVLA"
     gene            complement(5686..5994)
                     /gene="fdxB"
                     /locus_tag="PKV56_13915"
     CDS             complement(5686..5994)
                     /gene="fdxB"
                     /locus_tag="PKV56_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HOX68956.1"
                     /translation="MSSFSVTLPSGESWTPQFVQHINEETCIGCGRCFKVCPRGVLSM
                     VGLNEDGERVGVSQDDDDDDEEYEKKVMTIAHQELCIGCTACSKICPKKCYTHAPMEG
                     "
     gene            complement(6012..6227)
                     /locus_tag="PKV56_13920"
     CDS             complement(6012..6227)
                     /locus_tag="PKV56_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HOX68957.1"
                     /translation="MGRPGAADLDDLKAQAKKLNAKATQAKMDLHDLSEELPTNWEQI
                     LDVAQRAHEAHAALMSVRQQLAAATPS"
     gene            complement(6283..6762)
                     /locus_tag="PKV56_13925"
     CDS             complement(6283..6762)
                     /locus_tag="PKV56_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HOX68958.1"
                     /translation="MTEAIAMEAPSDEVLLQDPFIRELIKQIRAQDTHGTWEGKSDAK
                     LLEPYILTAEQRRALPMMGDPDPETLWRLDLFHNAIGLSIERATGCMVSPMMKMSHEG
                     FGRAVLTAGRLIVVNRHLRDVHRFGYASLAKLAEAGNKLVNEGIGMVETYKEVANYG"
     gene            complement(6839..7255)
                     /gene="nifX"
                     /locus_tag="PKV56_13930"
     CDS             complement(6839..7255)
                     /gene="nifX"
                     /locus_tag="PKV56_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HOX68959.1"
                     /translation="MKIAFATQDQQRVDAHFGWAKYLAVYQIDAAGYQFVQSFPFGED
                     LAEDGNEDKLAPKLEAIKDCAIVYVAAIGGSAAARVVASKIHPIKVTQPEPILDLLDR
                     MQEVLKGTPPPWLRKALLKDQQPAHDFEDDEEEVKS"
     gene            complement(7277..8734)
                     /gene="nifN"
                     /locus_tag="PKV56_13935"
     CDS             complement(7277..8734)
                     /gene="nifN"
                     /locus_tag="PKV56_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HOX68960.1"
                     /translation="MAHVVESKKACAVNPLKMSQPLGASYAFMGLESCMPVMHGSQGC
                     TSFGLVVLVRHFKEAIPLQTTAMNETTTIMGGYDNIEKAILNIRSRAKPQVIAICSTG
                     LTETKGDDVDGYIALARQKHPELADTEIIYVSTPDYLGAFQDGWAKAVTTLVTKVPRL
                     DTPRCEGRINVLPGSHLTPGDIEELREIIEAFGLEAAFVPDVSGSLDGHIPDDWLGTT
                     IGGTPLKALQEIGGAAHTLAIGEQMRPAAEALQARCGVPFTVFDRLTGLTATDALVQL
                     LGSLSGRAAPPRLRRQRSQLVDAMLDGHFHFGNVKVALAAEPDLLFAVGSFLTEMGAE
                     LAVCVTTTASPLLARMPAVEVVIGDLEDFEQAAQASGCDLLMTHSHGRQAAERLGKPL
                     FRLGIPTFDRIGNSHKCYVGYRGTRNLVYEVGNLLMEQIPHHGPDHWPLPEAALAAAR
                     GSAMPMFSTLPEPGVAHVSTEIPRLLHSSEPVSAP"
     gene            complement(8753..10198)
                     /gene="nifE"
                     /locus_tag="PKV56_13940"
     CDS             complement(8753..10198)
                     /gene="nifE"
                     /locus_tag="PKV56_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HOX68961.1"
                     /translation="MSASLKAKIAEVFEEPGCDKNVGKTEKERKKGCTKQLTPGAAAG
                     GCAFDGAKIALQPIVDVAHLVHGPIACEGNSWDNRHSASSGSTIYRTGFTTDINELDV
                     IYGAEKRLFKSIREICEKYDPPAIFVYQTCVTGLIGDDIEAVCKRAAEKFGKPVIPVN
                     APGFAGPKNLGNKLGAEALLDYVIGTVEPTHTTPYDINIIGEYNLSGELWQVKPLLDA
                     LGVRILSCISGDGRYAEVASSHRAKANMMVCSKSMINIATKMQQRWDIPYFEGSFYGI
                     GDMSETLREIARLLVERGAPAELKDRTEALIAVEEARAWERIRAYKQRLTGKKVLLIT
                     GGVKSWSVVAALQEAGLEVVGTSTKKSTKEDKEKIKEIMGDDAHMIDDMTPRQMYAML
                     REAKADIMLSGGRSQFVALKARMPWMDINQERHHAFAGYEGMVTMIAEIDKALFNPMW
                     AEVRTPAPWELVDPLLVEQSAEQLAAEAQPA"
     gene            complement(10452..>10700)
                     /locus_tag="PKV56_13945"
     CDS             complement(10452..>10700)
                     /locus_tag="PKV56_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68962.1"
                     /translation="EQRIDHWTYAAIAAASGLVLCLVVVVGAGWMARSVLGLARGEDY
                     LISWPPGVSRGRRRRRTQAEASSGRVGRRSRSRPSRLE"
BASE COUNT         1630 a         3622 c         3653 g         1795 t
ORIGIN      
        1 gcacacggtg ggcgagttcg gcgtggtgct gatgatcggc ggcaacatcc ccggccagac
       61 gcgggtgctg tcggtggcga tctacgacca tgtcgaggcc gccgaattcg accaggcgca
      121 tcggctggcc gccggcatgg tggcgttcgc gctgatcgtt ctggtgacgc tgtacatcac
      181 caaccgaccg ccacgcgacg acgcggagat cgcccggtga tcgaagccca tctgcgcctg
      241 cagcggcgcg acttccggct cgaggtggcg cttgcgctgc catcacgcgg cgtgaccgcc
      301 ctcttcggtc catcgggctg cggcaagacc acggtgctgc gggcgctcgc cgggctcgag
      361 cgggcgtcgg ggcgcatcgc gctggacgac gaagtctggc aggacgatgc catcgggtgc
      421 ttcgtgccga cgcaccggcg gccgctgggc tacgtgatcc aggaggccgc actgttcccg
      481 catctggatg tgcgtggcaa cctggactac ggacgcagcc gggccgtgag cgagtccagc
      541 cgcgttccgc tcgacgcgat catcgaactg ctggggatcg ggcacctgca ggcgcgccgt
      601 gcggccacgc tctcgggcgg cgagcgccag cgcgtggcca tcgctcgagc actggccact
      661 ggtgcacggc tgctgctgat ggacgagccg ctggccgcgc tcgacgcggc gcgcaaggcg
      721 gagatcctgc cgtacctcga acgcctgcat cgcgagctgg ccatgccggt cgtctacgtc
      781 acgcactcga tggacgaggt ggcgcggctg gccgaccacc tggtgctgat gcgcgagggc
      841 cgcgtcgtgg cggccggcgc gctggccgat ctgatggcgc gcaccgacct gccactgagc
      901 cggctcgacg atgcgggcgt ggtgatcgtt gccgaggtgg ccgaacacga cgagctgcac
      961 ggcatgaccc gcatcgcgtt cgacggcaca tcgatgtggg tcggccagac cgccgcgcaa
     1021 ccgggtgaac aggtgcgcgc tcgcgtgctg gcccgagacg tgagcgtcac gcgtcaacgc
     1081 cccaccgaga cgagcatcct gaacgtgctg ccggttcgcc tcgacagcat cgagcgcgac
     1141 cagagcacgg ccctgttgcg gctgcaggcc ggagcggtga ccgggaccgg tggctggacc
     1201 ctgctggcgc gcatcacaac ccgcagcctg gacgcgctgg aactgcgacc cggcgatgaa
     1261 ttgcacgcgc agatcaaggg cgtggcactg atgtaggaga caaagatgga acgaattgcc
     1321 gaccactggc aggcgcacct gcccgacgac ctccacgaac acgtcgtgtg gcccgtgcgc
     1381 ttcgaggcgc accacgacga cggcgtgccc gcatcgaagc accggggcta cgacgccctg
     1441 gggctgctgt gctggtatcg gcaccacttc tcgcaatggg acgcctcgtt cgacgacaac
     1501 gaccaaccca cggcgcagtt gctgcgcgag gaagacttcg aggcctggcg caccctggcc
     1561 ggaccgtgga tacgccgggt tcagcggctc gacggcgacg gccacccatg cggatcgctc
     1621 agcgactcgg gcttcgaggt cgtcgagcct gcggccatcc cgcggctgtg atcacggccc
     1681 cttcatgcga ctgaaagcgg cccatggaac gcatcgacga cgatgaactc aggcgctggt
     1741 tgcgcgagga cgctccgcac ggcgacctga ccacgcgggc gctgcacatc ggcgatcggg
     1801 ctggtcaact tcgcttcgaa acccgcgcca gcatgcgcgt cgcggtcgtc gaggaggccg
     1861 cgcggctgtt cgagctctgc ggctgcgacg ccatcgtggt gcgcggcagc ggcagtgaag
     1921 cgcagcccgg tgatccgctg ctgacggcca ccggcccggc cagcggcctg ctgctggcgt
     1981 ggaaggtggc gcagaccgcc gtcgagacgg ccagcggcat cgcatccgaa gctgcacgca
     2041 tcatcgcctt gctgcgcgac gcgggcttcg gccagccgct ggcctgcacg cgcaagaact
     2101 ggccgggcgg acgtgccctg gccgcacgcg gcgtgtgggc gggcggcgcg gtgatgcacc
     2161 ggctggggct gtcggagacg ctgctggtct ttcccgagca ccgcagcttc cttgcaccga
     2221 cagagatcgt ctcgtcgatg aaggctctgc gctcggcaca gccggaaaag aagctcgtcg
     2281 tggaagtggg ctcggtggat gaagcgatgt gcttcgccca ggcgggcgcc gaggtcctgc
     2341 aactcgaacg attcagcccg gctgcgctga ccgctttgcg ccagcgcctc gatgccgctg
     2401 cgctgcgccc cctgctggct ccggccggcg gcgtgaccgc cgccaatgcg ctcgactatg
     2461 cacgggcggg agccgacttc ctcgtgagtt cggcgcccta cttcgcaccc ccagccgatg
     2521 tgaaggtcgt catcgggccg tcccggctct gacgctgatg ctgaccgagg aggtcggatg
     2581 ccgggactcg atcgtgcagc gcatttgcaa tacactgact ccatgaagtc cagcaccatc
     2641 ccgtccgttc gcgtcgaacc cgagttccgc gccgaagtgg aggccgtgct gtcgacgggc
     2701 gaaaccttgt ccgagttcgt ggaatcgtcg gtgcgggcca gcgtcgaacg tcgtcgagcg
     2761 caggccgagt tcatcgcacg cggcttgcag tcgcgcgacc aggctcgcca gacgggcgat
     2821 tacgtcgacg ccgatgtcgt cctcgaaggt ctgcagcgca agctcgatgc cgtgcatgcg
     2881 cggctggcaa agacccgcaa gtgagtttca gggtccggct gacgcgggac gccgcggcgg
     2941 atctcgatcg actgttcgac ttcgtgatcg aacgcgagct ggcccgggag ggcggagacc
     3001 tgactatggc cgagcaggcc atcgctgcgc tgcgggcggg catcgccacg ctgaagtcgt
     3061 cgccgttcac ctgccgcaag gcgggccaga gccccttcct gcgcgaactg gtcattccgt
     3121 tcgggcgctc gggctacgtt gcgttgttcg agatcgagga tgcgtcgaac gtcgtcgtgg
     3181 tggccgtgcg ccatcagcgc gaggacgact accactgaag agggcggtcg ggctcctcga
     3241 gcgcacgagc cctgcgaagc cagtcgcaag aacccgaacg tcctgcgttc aggctggcgc
     3301 gccgaacacg gccggatcct gctgccactg ttcgagcacg cggccgcaga gcgctccgtc
     3361 ggcgtcgaag cttcgctcct cgaaatgcag gctgccgtcg cggcccatga tgaagaccgt
     3421 gctgctgcgg gtgccggtgt cgccgcctgc gatgaacacc gccgagcgcc gctgctcttc
     3481 ttcgggatcg aggccggtct cgggcaggcc ggcggccgac gtgtggcgat cggcgagcat
     3541 cacgaacgcg gcctcggcga tgtcggacgc gcacagcacg tcgtcgggca ccggcagctc
     3601 gaagccgcac agggcctgcg cgtcgcgcag cttcggatag ccgtcgagca gcgtggtgaa
     3661 gccgccgtgc gccttgatgt aggccccgaa cagcgtgccc agccgctcgg tctgcggcca
     3721 gcgctggtcg agcagtccgt tggacagcgt gtgcagaccc tcggtcaggg cgatcggcag
     3781 tcgcgcacgc gtcgcggcgt aatgcgcctg gcgcgggttg ccgacgatca ggttgaatgg
     3841 ggcatagggg cccttgtcgc gcatgaagcg gcgcgcgaag accaccggat cctcgcccga
     3901 ggccaggtag tccagcggca gaccgccgcg gctcggcgcg ccggccggcg gcgggctggc
     3961 ttcgcgatag ccggtgacca gcgcaaagcg accgccctcg cggctcacgg ccagccaggt
     4021 gccctgcccc gccaggtcgc gaccggccag cacgccggcg ggctcggtcc accacgcggc
     4081 gggctcggtc gcgcggctac gcgcctcggt gcggttgaag agcagcacca gcggaaagta
     4141 cgggtgcgcc cgccatgcaa tgtcgaccac gctcatgagc tgttcctcag ggtgacttga
     4201 aggcgatggc cacgatctgc atcgcgtcgt ggcggatgcc gtcgggcccg cgcagggcct
     4261 cgagccggcg caggatctcg gcccgcgcgc gatcgcgcag gtcgggcggc aggtgcatca
     4321 ggaaatggcc ccaggcgctc gcctgcatgt gctcgagcgc gccttcaccc gtcgcatgca
     4381 ggaaggtctc cggtcgcgcc tcgacctgca ccgacaccag gccggcgccc cgcagcaggc
     4441 gatccagttc gtcggcgccg accgggtagc ggcgactctc cgccggcggc gggtcatccg
     4501 catagggcgg ctcggccatc agctcgcgtt gcaggcgctc ggcaggatgc ggatgcgccg
     4561 ccgattgggt tgccaagccc aggcagcccg cgggcgccag cagccgcgcg cattcggtca
     4621 gcgcggcggc cgcatcgggc caggtgtgca gcacgccatt ggcaacgatg gcatcgaagc
     4681 tgcccgcacc gaagcgctgc agcgagcaag gatccccgac ctggaatcgc aggttgtagc
     4741 gcccgcgctg atgcgcgatc tgcacccgca gcgccatcgc gtcgagaccg agcacctcgc
     4801 cttgcgaacc gacgcgttcg accaggtgct cggccagcag tccggttccg cagccgagtt
     4861 cgagcacgcg ctgaccggcc cgcagaccca tgcggtcggc cagggcgcag ccggccttca
     4921 gctgccgttc gaccgccagc cactcgtgat acgacgccag cgccgggatg ccatccaccg
     4981 gagtcaggcc aagaccgcgt cttccggtcc gaaacagtgt gcgtagtcgg tgcagacgct
     5041 gcagctcgga gcccggcaga tcgtgagctc ggcccgctcg cacagctgct tgtagaggaa
     5101 cttcttccac ttcatgtcgc ggtcgttgcg cgccgcgatc gcgggaaacc agccactcat
     5161 caatgcagac aactctcgcc gcgaaggcag gtgcaggtcc tgccacaggt ggttgtctcc
     5221 caggctcgcc tggctcagtg catgagccac ccagatcacc tcctcgcgcc cggcatgcgt
     5281 tgggcaaacg cccgtcgtca acagaccgac cagatcctcg atctcgtcgt agcgaggctc
     5341 gtcgcgcgtg gcgtgcctga gttcctgcca gtccagagcc aacaaccgat cggcgcccgg
     5401 aaaccatcgc gccatcatcc ggcgcgtggc cgaatcggag agcccggaaa tcggcagctg
     5461 atcgaaaccg tgcacctcga acgccgtggc cagcaccccg gccatcgcct gcacttcggg
     5521 tgcatccgcg gccgtcgcgc aactcatcag gtcgagccag atcgtcagcc tcaggccctt
     5581 cggcatggca ccgtgactgg tgcaagccac gcctgccggg ttctgccacc gacccacgac
     5641 ggtgatgggt gaaatctggt tgagggcttg catggcgcgc actcctcagc cttccatcgg
     5701 ggcatgcgtg tagcacttct tcgggcagat cttcgagcac gcggtgcagc cgatgcacag
     5761 ctcctggtgc gcgatggtca tgaccttctt ctcgtattcc tcgtcgtcgt catcgtcgtc
     5821 ctgcgagaca ccgacccgct cgccgtcctc gttgaggccg accatcgaca gcacgccgcg
     5881 cgggcagacc ttgaagcagc gtccgcaacc gatgcaggtt tcctcgttga tgtgctggac
     5941 gaactgcggc gtccagctct cgccgctggg cagcgtgact gagaaagaac tcatctcgat
     6001 gctcccttcg atcaggacgg tgtcgcagcg gccagctgct ggcgaacgga catcagcgcg
     6061 gcatgtgctt catgcgcgcg ctgcgcgaca tcgagaatct gctcccagtt ggtgggcagc
     6121 tcctcggaca ggtcgtgcag atccatcttg gcctgcgtgg ccttcgcatt gagcttcttc
     6181 gcctgcgcct tcaggtcatc caggtcagcg gcgccgggcc gccccaaggc gctgacggca
     6241 ccctcggggg gtaccgaacg aagcgaggtc gggggctcca tgtcagccat agttcgccac
     6301 ctccttgtag gtctcgacca tgccgatgcc ctcgttgacc agcttgttgc cggcctcggc
     6361 gagcttggcc aggctggcgt agccgaagcg gtgcacgtcg cgcagatgtc ggttgacgac
     6421 gatcaggcgg cccgccgtga gcacggcgcg gccgaagccc tcgtggctca tcttcatcat
     6481 cggactgacc atgcagccgg tggcgcgctc gatcgacaga ccgatcgcgt tgtggaacag
     6541 gtcgagccgc cagagcgtct cgggatcggg gtcgcccatc atcggcagcg cgcggcgctg
     6601 ctcggcggtc aggatgtagg gctcgagcag cttggcgtcg ctcttgcctt cccacgtgcc
     6661 gtgcgtgtcc tgcgcacgga tctgcttgat cagttcacgg atgaacgggt cctgcagcag
     6721 cacctcgtcg ctcggtgctt ccatcgcgat ggcttcggtc atgccgacct ccgacgagcc
     6781 gtctctaggt ggtcgggcgc cccctcgggg ggcagcgaac gcatgtgagc gtgggggttc
     6841 atgatttcac ctcttcttcg tcgtcttcaa agtcgtgggc gggctgctgg tccttcagca
     6901 gcgccttgcg caaccagggc ggcggggtgc ccttgagcac ctcctgcatc cggtcgagca
     6961 ggtcgaggat cggctcgggc tgcgtcacct tgatcggatg gatcttgctg gccaccacac
     7021 gcgccgcagc cgatccaccg atggccgcga cgtagacgat cgcgcagtcc ttgatcgcct
     7081 cgagcttcgg cgcgagcttg tcctcgttac cgtcttccgc caggtcttcc ccgaacggga
     7141 aggactgcac gaactggtag ccggcggcgt cgatctggta gacggcaagg tacttggccc
     7201 agccgaagtg ggcgtcgacg cgctgctggt cctgggtggc aaaggcaatc ttcatcgtgg
     7261 ctccttgggt agggaatcag ggggcggaca ccggctcgct gctgtgcagg aggcggggaa
     7321 tctcggtgct gacgtgcgcc acgccgggct cgggcagggt gctgaacatc ggcatcgccg
     7381 aaccgcgcgc agccgccagg gcagcctccg gcagcggcca gtgatcgggc ccgtggtgcg
     7441 ggatctgctc catcagcagg ttgccgacct cgtacacgag gttgcgcgta ccgcgatagc
     7501 cgacgtagca cttgtgcgaa ttgccaatgc ggtcgaacgt cgggatgccc aggcggaaca
     7561 gcggcttgcc cagacgctcg gccgcctgcc gcccgtgcga atgcgtcatc agcaggtcgc
     7621 agccgctggc ttgcgcggcc tgctcgaaat cctcgaggtc gccgatcacc acctcgaccg
     7681 caggcatgcg cgccagcagc ggcgaggccg tcgtcgtgac gcagaccgcg agctcggcac
     7741 ccatctcggt caggaagctg ccgacggcga acagcaggtc gggctcggcc gcgagcgcga
     7801 ccttgacgtt gccgaagtgg aagtgcccgt cgagcatcgc atcgacgagc tggctgcgct
     7861 gcctgcgcag gcgcggcggc gcggcacggc cggagaggct gccgagcaac tgcaccagcg
     7921 cgtcggtggc ggtcaggccg gtcaggcgat cgaacaccgt gaacggcacg ccgcagcggg
     7981 cctgcagcgc ctcggccgcc ggccgcatct gctcgccaat ggccagcgtg tgcgccgcgc
     8041 caccgatctc ctgcagcgcc ttcaaaggcg tgccaccgat cgtcgtgccc agccagtcgt
     8101 cgggaatgtg gccgtcgagc gagcccgaga cgtcgggcac gaacgccgct tccaggccga
     8161 aggcctcgat gatctcgcgc agttcctcga tgtcgcctgg cgtcaggtgg ctgcccggca
     8221 gcacgttgat gcggccctcg cagcgaggcg tgtcgagtcg cggcaccttg gtcaccagcg
     8281 tcgtgacggc cttcgcccag ccgtcctgga aggctcccag gtagtcgggg gtcgagacgt
     8341 agatgatctc ggtgtcggcc agctcggggt gcttctggcg cgccagtgcg atgtagccgt
     8401 cgacgtcgtc gcccttggtc tcggtgagac cggtcgagca gatcgcgatg acctgcggct
     8461 tcgcgcggct gcggatgttg aggatcgcct tctcgatgtt gtcgtagcca cccatgatgg
     8521 tggtggtctc gttcatcgcg gtggtctgca gcggaatcgc ctccttgaag tggcgcacca
     8581 gcaccaccag gccgaacgag gtgcagccct gcgagccgtg catcaccggc atgcatgact
     8641 cgagccccat gaacgcgtag ctcgcgccca gcggctggct catcttgagc ggattgaccg
     8701 cgcaggcttt cttcgattcg acgacgtggg ccatggtgtg tgctccgtcg ggtcaggcgg
     8761 gctgtgcttc ggcggcgagc tgctcggcgc tctgctcgac cagcagcggg tcgacgagct
     8821 cccacggggc gggcgtgcgc acctcggccc acatcggatt gaacagggcc ttgtcgatct
     8881 ccgcgatcat cgtgaccatg ccctcgtagc cggcgaaggc gtggtgccgc tcctggttga
     8941 tgtccatcca gggcatgcgg gccttcagcg cgacgaactg gctgcgcccg ccgctgagca
     9001 tgatgtcggc cttcgcctcg cgcagcatcg cgtacatctg gcgcggcgtc atgtcgtcga
     9061 tcatgtgcgc gtcgtcgccc atgatctcct tgatcttctc cttgtcttcc ttcgtgctct
     9121 tcttggtgct ggtgccgacc acctcgagcc cggcctcctg cagcgcagcc acgacagacc
     9181 agctcttcac gccgccggtg atcagcagca ccttcttgcc ggtgagccgc tgcttgtagg
     9241 cgcggatgcg ctcccaggcg cgagcttcct cgaccgcgat cagcgcttcg gtgcggtcct
     9301 tgagttcggc cggcgcgccg cgctcgacga gcagccgcgc gatctcgcgc agcgtctcgc
     9361 tcatgtcacc gatgccgtag aacgaaccct cgaagtacgg gatgtcccag cgctgctgca
     9421 tcttggtcgc gatgttgatc atcgacttcg agcagaccat catgttggcc ttcgcgcggt
     9481 gcgacgaagc cacctcggcg tagcgtccgt cgcccgagat gcagctgagg atgcgcaccc
     9541 ccagcgcatc gagcagcggc ttgacctgcc acagctcgcc ggagaggttg tactcgccga
     9601 tgatgttgat gtcgtagggc gtggtgtgcg ttggctcgac ggtgccgatc acgtagtcga
     9661 gcagcgcctc tgcgccgagc ttgttgccga ggttcttcgg cccggcgaag cctggcgcgt
     9721 tgaccgggat caccggcttg ccgaacttct cggcggcgcg cttgcacacc gcttctatgt
     9781 cgtcgccgat cagtccggtg acgcaggtct ggtagacgaa gatggccggc gggtcgtact
     9841 tctcgcagat ctcgcggatc gacttgaaca ggcgcttctc ggcgccgtag atcacgtcga
     9901 gctcgttgat gtcggtggtg aagccggtgc gatagatcgt cgatcccgac gaggcgctgt
     9961 ggcggttgtc ccacgagttg ccttcgcagg cgatcggccc gtgcacgagg tgcgccacat
    10021 cgacgatggg ctgcagggcg atcttggcgc cgtcgaacgc gcagccaccg gcagctgctc
    10081 cgggtgtcag ctgcttggtg caacccttct tgcgttcctt ctcggtcttg ccaacgttct
    10141 tgtcgcagcc aggctcctcg aacacttcgg cgatcttggc tttcagcgac gcagacatcc
    10201 ggtgactcct gcagtgtgta gcgacatcga accggggggt tcggaggtgc tattgcagct
    10261 tcagtgccag acgcgtaaat ccctacaaat caagtacttg gacgattcgg cgggtccatc
    10321 ccggcccacg ccgagcgaca gcgggctgcg gcaattggca ggttcccgac aatcacggca
    10381 caccggtccg gtgcctttgc cgatccgcat cgaactgcac ggcccgggcc tgccgggacc
    10441 ggcggccagg ctcattcgag ccgggaggga cggctccggc tgcgtcgccc gacgcggcct
    10501 gatgaggctt ccgcctgcgt gcgtctgcgt cgcctgcctc tggaaacacc cggtggccag
    10561 gagatcaggt agtcctcgcc cctggccagg cccagcacgc tgcgggccat ccagccggcg
    10621 ccgacgacga ccaccaggca caggaccaga cccgacgccg cggcgatggc cgcgtacgtc
    10681 caatgatcga ttcgctgctc
//
LOCUS       DAOORV010000120        10175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8218_length_10175_cov_11.007806, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000120 DAOORV010000000
VERSION     DAOORV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_10175_cov_11.007806"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2015
                     /locus_tag="PKV56_13950"
     CDS             <1..2015
                     /locus_tag="PKV56_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HOX68963.1"
                     /translation="LRKCRDWASEIGEIKITEDQNPIISIQVTGVDIEPILRAAEAND
                     NAGNRRKRIREALFKELEIPDTGGLFTTYEFNWRGTRREVELLYENVREMTDDRLRGR
                     SGAWTVVLDFPFDDANFGPADDLARLAEYRGGDTKTLVWLPSFLSNKALADLGRLVVL
                     DYILQGERFDNYAAHLSFVDRVQAKALARNQLDQLRIKLRSQLEVAYGISTEPRDAVS
                     NPLTADQQFKSLDPTLSPRPPVGAEFKSAFESLLGQLFAHQYPAHPEFDTEIKPSVIK
                     KVWPEVQKAIEAPGQRGLVQDTSTRKLVRAVVNPCQLGQMGETHLLIEPHWQSRFSQS
                     HARDGGGAITVAKLRQWIDTPAPMGLPIELQNLIILAFAASTNRRFTMRGGPYEPTID
                     SLPDELELKEQALPNTADWETALLRASSLFGLTLGQTLNAANVGKLVDEVRQKAADKR
                     EAVARLVSQVRDRSARYAPGITGARQQSAESAQALLASLAQAAEGDVVTTLANASLQT
                     SEAAVSRSLGQAQVCADALGSGNWQLFDVVRDLVDHRRDAALLIMSRLTEALTSDEHV
                     VALKPRLEELARDAMRLLAAAAPAPVTPPPVAPTPPGAGPAPPPVVMPPAPPANGPEV
                     VDEKQQLHLSGAAAVTALDELKTLVTSERDLELTLSWRVQRKGTKL"
     assembly_gap    2018..2117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2118..>4561
                     /gene="pglZ"
                     /locus_tag="PKV56_13955"
     CDS             2118..>4561
                     /gene="pglZ"
                     /locus_tag="PKV56_13955"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HOX68964.1"
                     /translation="MSLSTPQIAAQLERVLASDPAAVAIAIRANAKQPWPDSLKQQGR
                     DFQLRWCDSSLAIREALCDVEQHDPMTSGLVVITPLATHEVAEDISARLARARVFQPE
                     GWDIVRQLFQAKETDARLGRYVWMPQTLIDGASQGTYPPVANGFLDLETAWREVLHRF
                     LGIEAARPDAVALLLWAMDPAADPRLAQLPAPARVDVMRWLGETAGVAGEMVLGCVDA
                     GRTIDALPLGLVCGVVFAQDGEGQASLGQAAIRLERFVNGKHVGVAEGRAWARAAEQV
                     VRLTGLDASRAVLDRADALLRDLRVIDFAHLSDVLPAALDQRLEDFALALTAHVAEPS
                     ESNLAQVELQADRALRHTLVAAQKPRMERVEMARRLARWLMNATPTASSLQTAVAWQS
                     DQGAFVDWARFRLLGGDELPDLSQAYSACREAVIARRNAFAKPFAQALVQWNAQKPAP
                     EGRIIPLESVLEHVLAPIAAAHPTLLLVMDGLSTSIFRELFARIASQGWAEMVPSSLG
                     QPLAGVAALPTITEVSRASLLCGRLTVGASAQEKTGFATNAALLAHSRADAPPRLFHK
                     GDLADATNLAPEVRAAIASTQQRVVGVVYNAVDDHLSGPDQLHQRWALEDLRLLLPLL
                     REAREARRVVVITADHGHLLEDGTTQLPGGESDRWRPGKNAETPNEVAVSGGRVVTSD
                     GSNAVVCLWGESTRYAGRKNGYHGGLSPQEVTVPLSVIVPLGFNLTGWTPAPPAPPEW
                     WELPPMPKSSGVQSAAAAAPKPTRRKPTAQQGQPQLFEPVDLPPPEASTAPVALDWIG
                     ALLSSPIYASQRQLAAR"
     assembly_gap    4562..4661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4662..5986
                     /gene="brxD"
                     /locus_tag="PKV56_13960"
     CDS             <4662..5986
                     /gene="brxD"
                     /locus_tag="PKV56_13960"
                     /inference="COORDINATES: protein motif:HMM:NF033438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HOX68965.1"
                     /translation="GRPTMISSARREDIVSALRRGTVPSSGLDALAVGIDTFAPTLDD
                     ELESVSAGRGGFKAVRGEYGTGKTFFGRWLQERARARGFAATEVQINETETPLHRLET
                     VYRRLVEHLGTADTASGAFRGVIDGWFYALEQDVLADASVDASDAEGLLLRTNALMEA
                     RLGAISKTAPAFSAVLRTYRRALAAGDGMLADGLISWLAGQPNVAASIKRAAGIKGDL
                     DHFGATNFLVGLLTILRDSGFSGLVMVLDEVETLQRMRTDTREKGLNALRQWIDEIDA
                     GRYPGLYLVITGTPAFYDGPQGVQRLAPLAQRLHVDFGTDRRFDNPRAVQIRLAAFDH
                     TSLLAVGRRVRDIYADGRPNEQRLRSVVDDSYLDALARAVAGGLGGKVGVAPRLFLKK
                     LVSDVLDRVDLHEDFDPRKHYTLTLAETEMCATERAAASAASVDDIEL"
     gene            5983..>6539
                     /locus_tag="PKV56_13965"
     CDS             5983..>6539
                     /locus_tag="PKV56_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX68966.1"
                     /translation="MNEFEQLHPSLQYHVVNTLGWSTLRPTQLAAIAPIHAGSHCLLL
                     APTAGGKTEAAAIPMLSRMLTEAWPGTSVLYICPIKALLNNLEHRLTHYAGLVGRRVE
                     VWHGDISQSRKNRALRDAPDILLTTPESIEAMLISVRVDRPAWFGNLRAVIVDELHAF
                     AADDRGWHMRSVLHRLDQYLERPLQR"
     assembly_gap    6540..6639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6640..7881
                     /locus_tag="PKV56_13970"
     CDS             <6640..7881
                     /locus_tag="PKV56_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOX68967.1"
                     /translation="RAKEMRTFVSHASLSVSERRQAEAAFTEEKDCVIVATSTLELGI
                     DVGDLDRVIQIDSPSSVSSFLQRMGRTGRRAGALRNCLFLTTNDDAFMLALGVTKKWS
                     EAWVEAAVPPAEPWPILAQQALVLVLERGEVPTMEVVQLLHGSFPELATDDITALVEH
                     LVDKQFLDRSEGVVRVGPETERVYARGHYRDLLASFSGSMLLTGRHGSSEVGYIDPTV
                     LTGEQDNRLLLLAGRSWRVTEVEWSKRIVWLEPAREGGKARWMGGARSLGRDVCQAIR
                     TVLASGAPPIVTLSQRARAALSSLTDELPMTLGAHFVMARSDAAPVRTWTFAGTRANR
                     TWAHQASVGGQKVRFDAMSVHAPASLLADAAPGQLTLTDAEIATFAESVKFAECVPRG
                     LLTRTIVARNFESPIRWEPTP"
     gene            7863..8852
                     /locus_tag="PKV56_13975"
     CDS             7863..8852
                     /locus_tag="PKV56_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68968.1"
                     /translation="MGTDTMSAARLSQDDYPEVGARRRGHILVCHGDDSPDQWIAWLE
                     PIQSRFKELHGLSEEYGFALDAGEESGRLGSVGEFLELIGVDIRSNPWEGSLISGVYR
                     FGWVKVGTVRAVRKKAAELLKALQRQVDSEVRRKILEDLAGIKGIDQLIERTAADRDE
                     LRRQILESKSALSKAQEDLRLTRDRLQQLEVSDSKQIDESCLRMAGAILRRAPCRMVA
                     GPLVLWDDNVVYAITSRGGVTLPANSTPAILLGDLEAAGYPVVRVEEKTVIGGWTSGP
                     IYRKGTPHITGWASVWSDRERKERVFLVVPKDSERKVHEIHQALASGFSGASK"
     gene            8849..9829
                     /locus_tag="PKV56_13980"
     CDS             8849..9829
                     /locus_tag="PKV56_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68969.1"
                     /translation="MRENVTMECGHSPAPGMDWNVTVSFVGNAKMYRVWSRSDEDQNR
                     VLLKQAAGPLTWRRMVADIKELDEIGLGGDLLSDIQVSGLTGWQSSLLKMAWCKFEGP
                     AERQLEFLLDLPDETIALLEEKLGDLTDPDVLAALDEVIEAKEELQISMAQVDQAINE
                     TTKAMRKGLDPWVRSMSDWFQAHEVIDSGPGVSMEVARRSTTAETLAIVTTMQSPLAV
                     EVARVLLVNWSKAEPKPVQEGRVGGIRFRSTIPFLQWLVQRLEPGALASVTAHEDGDE
                     LNAMEALDAHIAAEIHRLRTSSGGGALGHGVRGGIVASWDRLRRQVFGPG"
     regulatory      9905..10011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
BASE COUNT         1771 a         3056 c         3378 g         1670 t
ORIGIN      
        1 tgctgcgcaa gtgccgcgac tgggccagcg agatcggcga gatcaagatc accgaagacc
       61 agaacccgat catctcgatc caggtgacgg gcgtggacat cgagccgatc ctgcgggcgg
      121 ccgaggcgaa cgacaacgcc ggcaatcggc gcaagcggat ccgggaggcg ctgttcaagg
      181 aacttgagat tccggacacc ggtggcctgt tcaccaccta cgaattcaat tggcgcggca
      241 cccggcgcga ggtggagctg ctgtacgaga acgtgcgcga gatgaccgac gaccggctgc
      301 gcggccgctc gggcgcctgg acggtggtgc tggactttcc gtttgacgat gcgaacttcg
      361 gcccggctga cgacttggcc cgcctggcgg agtaccgcgg cggcgacacc aagacgctgg
      421 tatggctgcc ctcgttcctg agcaacaagg cgctggccga cctggggcgg ctggtggtgc
      481 tggactacat cctgcagggt gagcgctttg acaactacgc ggcccacctc tcgttcgtcg
      541 accgggtgca agccaaggcc ctggcgcgca accagcttga ccagctgcgc atcaagctgc
      601 ggtcgcagct ggaggtggcc tacggcatca gcaccgaacc gcgtgatgcg gtgagcaacc
      661 cgctgacggc ggaccagcag ttcaagtcgc tggatccgac gctgtcgccg cggccaccgg
      721 tgggggcgga attcaagtcg gccttcgaga gcctgctggg gcagttgttt gcccaccagt
      781 acccggccca cccggagttc gacaccgaga tcaagcccag cgtcatcaag aaggtgtggc
      841 ctgaagtcca gaaggccatc gaggcgcctg gccaacgcgg gttggtgcag gacacctcga
      901 cccgcaagct ggtgcgtgcg gtggtcaacc cctgccagtt ggggcagatg ggtgagaccc
      961 atttgctgat cgagccgcac tggcaatcgc gcttctcaca aagccatgcg cgggacggag
     1021 gtggcgccat caccgtcgca aagctacggc agtggatcga cacccctgcg ccgatgggcc
     1081 tgcccatcga gctgcagaac ctgatcatcc tggcctttgc ggcatcgacc aaccgccgct
     1141 tcacgatgcg cggcgggccg tacgagccca cgatcgacag cctgcccgat gagctggagc
     1201 tcaaggagca ggcgctgccg aacacggcag actgggagac tgcgctgctg cgcgcgtcca
     1261 gcctgttcgg gctgacgctc ggtcaaacgc tcaatgcggc caatgtcggc aagttggtcg
     1321 atgaggttcg gcagaaggcg gcagacaagc gtgaggcggt agcccgcctg gtatcgcagg
     1381 tgcgcgaccg gtcggctcgc tacgcgcccg gcatcaccgg ggcacgccag cagtctgcag
     1441 agtctgccca ggcgctcctg gcctcactgg cacaagcggc agagggcgac gtggtgacga
     1501 cgctggccaa tgccagcctg cagacgtccg aggcggcggt cagccgcagc ctggggcaag
     1561 cgcaggtctg cgccgatgcc ttgggcagcg gaaactggca attgttcgat gtggttcgcg
     1621 acctggtgga ccaccgtcgc gacgcggcgc tgctgatcat gagccgcctg accgaggcgc
     1681 tgaccagtga cgaacatgtg gtggccttga agccgcggct tgaagagctg gcacgggatg
     1741 cgatgcgtct gctggcagcg gcagcgccgg caccagtgac gccgccaccc gtcgcaccca
     1801 cgccgcccgg tgccgggccg gcgccgccgc ccgtggtgat gccccctgcc ccgcccgcca
     1861 acgggccgga ggtggtggac gagaagcagc agttgcacct gagtggcgct gctgcggtga
     1921 ccgcgctgga cgaactgaag acgctggtca ccagtgaacg cgacctcgag ctgacgctga
     1981 gctggcgggt gcagcgcaag ggcacgaagc tatgagcnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnatg agcttgtcga cgccgcagat cgcggcccaa ctcgaacgcg
     2161 tgctggccag cgatccggcc gcagtcgcca ttgcgatccg ggccaacgcg aagcagccct
     2221 ggcccgattc cttgaagcag caggggcggg atttccagct tcggtggtgc gactcgtcgc
     2281 tggccattcg cgaagccttg tgcgatgtcg agcagcatga cccgatgact tctggactgg
     2341 tggtgatcac gccgctagcc acccacgagg tggccgagga catctccgcc cgtctggcca
     2401 gggcacgggt gtttcagccc gagggctggg acatcgttcg ccagttgttc caggccaagg
     2461 aaaccgacgc gcgcctgggg cgctacgtgt ggatgccgca gaccctgatc gacggcgcgt
     2521 cgcagggcac ttacccgccc gtggccaatg gcttcctcga ccttgaaacc gcatggcggg
     2581 aagtgctgca tcgattcctg ggcattgaag cagcacgccc cgacgcggtg gcgctgctgc
     2641 tctgggcgat ggacccggcc gccgacccca ggcttgccca gttgccagcg ccagcccgtg
     2701 tcgacgtgat gcgctggctg ggcgagacgg cgggagtggc cggggagatg gtgctgggct
     2761 gcgtggatgc gggtcgaacg atcgatgcat tgccactggg cctggtctgc ggcgtggtct
     2821 ttgcccagga tggtgagggt caagccagcc tcggccaggc agcgatccgc ctggagcgct
     2881 tcgtcaacgg caagcacgtc ggtgttgccg aggggcgcgc ctgggcgcgc gctgcagaac
     2941 aggtcgttcg actgaccggg ctggacgcgt cccgcgctgt tctcgaccgc gcagatgcac
     3001 tgctgcgcga cctgcgcgtc atcgactttg cgcacctcag cgacgtgctg ccggcggcgc
     3061 tggatcagcg actggaagac tttgcgctgg cactgaccgc ccatgtggcg gagccctcgg
     3121 agagcaatct tgcccaggtt gagctgcagg ccgaccgcgc gctccggcac accttggtag
     3181 ccgctcagaa gcctcgcatg gaacgagtcg agatggcgcg ccgattggca cgctggctga
     3241 tgaacgccac gccgaccgct tcatctttgc agactgcagt ggcctggcag tctgaccaag
     3301 gcgccttcgt cgactgggcg cgcttccggc tgctcggagg cgacgagctg ccggacctgt
     3361 cacaggccta cagcgcctgc cgcgaagcgg tcatcgcgcg ccgaaatgct ttcgccaagc
     3421 cattcgcaca ggcgctggtg caatggaacg cccagaagcc ggcgcctgag ggtcggatca
     3481 tcccgctgga gtcggtgctt gagcatgtgc tggcaccgat cgccgccgcc caccccaccc
     3541 tgctgctggt gatggatggt ttgagcacca gcatcttccg ggagttgttc gcccggattg
     3601 ccagccaagg ctgggccgag atggtgcctt ccagcctggg acagccgctg gctggtgtgg
     3661 ctgcactgcc aacgatcacc gaggtcagcc gcgccagcct gctgtgtggg cgtctgaccg
     3721 tcggcgcttc ggcgcaggag aagaccggct tcgcaacgaa cgctgccctt ttggcgcaca
     3781 gccgcgcgga cgcgccaccc cggctgttcc acaagggcga cctggcggat gcgaccaacc
     3841 tggcacccga ggtgcgcgcc gcgattgcga gcacccagca gcgtgtggtg ggcgtggtct
     3901 acaacgccgt ggacgaccac ttgagcggcc cggatcagtt gcatcaacgc tgggcgctgg
     3961 aggacctgcg gctgctgctg ccgttgctgc gagaggcgcg cgaggcgcgc cgggtggtgg
     4021 tgatcacggc cgaccatggg catctgctcg aagacggcac cacgcagttg cccggtggcg
     4081 agagcgaccg ctggcgcccg ggcaagaatg ccgagacgcc caacgaagtg gcggtcagcg
     4141 gggggcgcgt ggtcacctcc gacggatcga acgcggtggt gtgcctgtgg ggcgagagca
     4201 cacggtatgc gggtcgcaag aacgggtatc acggtgggct gtcgccccag gaagtaacgg
     4261 tgcctctgag tgtgatcgtg ccgctcggat tcaacctgac cggctggacc ccggcaccgc
     4321 cggccccacc ggagtggtgg gaactgccgc ccatgccgaa gtcgtcaggt gtgcaatcgg
     4381 cagccgccgc agcaccgaag ccaacgcggc gcaaaccgac agcgcagcaa gggcagcccc
     4441 agttgttcga gccggtcgat ctgccgccac cagaggcttc aacggcgccc gtggctttgg
     4501 actggatcgg cgccttgctg agcagcccga tctatgcgtc tcagcggcag ttggccgcgc
     4561 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagggaggc caacgatgat
     4681 cagttccgca cgccgggaag acatcgtgag cgccctgcga cgcggcacgg taccgagcag
     4741 cgggctggat gcgctggctg tcgggatcga tacgttcgcg ccgaccctcg acgacgagct
     4801 cgaatcggtc agcgccggcc gcggcggctt caaggcggta cgtggtgagt acggcaccgg
     4861 caagaccttc tttggccgct ggctgcagga gcgggctcgc gcacgcggct tcgcagccac
     4921 tgaagtgcaa atcaacgaga ccgagacacc gttgcaccgc ctggaaacgg tctatcgccg
     4981 cctggtcgaa cacctcggca ccgccgacac ggcaagtggc gcatttcggg gcgtgatcga
     5041 cggctggttc tatgccctgg agcaggatgt gctggcagac gcctcggtgg atgccagcga
     5101 cgcggagggg cttctgctgc gcacgaacgc gctgatggag gccaggctcg gcgccatcag
     5161 caagacggcg ccggccttct ctgccgtgct gcgtacctat cgccgcgccc tggcagccgg
     5221 ggacggcatg ttggcggatg ggctgatcag ttggctggca ggacagccga acgtggccgc
     5281 gagcatcaag cgtgcggcgg gcatcaaggg tgacctcgat cacttcggcg ccaccaactt
     5341 tcttgtcggc ctgctgacca tcctgcggga cagcggcttc tctggcctgg tgatggtgct
     5401 cgacgaggtg gaaacgctgc agcgcatgcg taccgacacc cgagaaaagg ggctgaacgc
     5461 cttgcgtcag tggatcgacg agatcgatgc tggccgctat ccggggctgt acctggtgat
     5521 cacgggaacg cccgcgttct atgacggccc gcaaggggtg cagaggctcg ccccgctggc
     5581 gcaacggctg catgtggact tcggcaccga tcggcgattc gacaacccga gagcggtcca
     5641 gatccggctg gcggccttcg accacacctc gctgctcgcg gttggacgcc gggtacgtga
     5701 catctatgca gatggtcgtc ccaacgaaca gcgccttcgg tcggtggtgg acgacagcta
     5761 tctggacgcc ctcgcacgcg ccgtggccgg cgggctgggt ggcaaggtcg gtgtcgcacc
     5821 ccggctgttc ctcaagaagc tggtgtcgga tgtgctggac cgcgtggacc tgcacgaaga
     5881 cttcgacccc cgcaagcact acaccctcac gctggccgag accgagatgt gcgcgacgga
     5941 gcgggctgcg gcgagtgcgg cgtccgtgga cgatattgag ctgtgaacga gttcgagcag
     6001 ctgcacccat cgctgcagta ccacgtcgtc aacacgctgg gctggagcac gctgcgaccg
     6061 acgcaactgg cagccattgc gcccattcat gcgggctcac actgtttgct gctcgcaccg
     6121 accgccgggg ggaagacgga agccgctgcg attccgatgc tgtcgaggat gctgaccgag
     6181 gcctggcccg ggacgagcgt cttgtacatc tgcccgatca aggcgctgct gaacaacctg
     6241 gagcaccggc tcacccacta cgccgggctg gtgggtcggc gtgtagaggt gtggcatggc
     6301 gacatctcgc agtcccgcaa gaaccgggcg cttcgagatg cccccgacat cctgttgacg
     6361 acacccgaat ccatcgaggc catgctgatt tcggtgcggg tcgatcggcc agcatggttc
     6421 ggcaacctgc gtgccgtgat cgtcgatgaa ctgcatgcgt tcgcggcgga tgacagaggg
     6481 tggcacatgc gctctgtgct gcatcggctg gaccagtatc tggaacgacc tctgcaacgn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcgccaagga gatgcgcacc
     6661 ttcgtgagcc acgcctccct cagcgtttcc gaacgccggc aagccgaggc ggcattcacc
     6721 gaagaaaagg actgcgtcat cgtcgccacc tcgacactgg agctgggcat agatgtcggt
     6781 gacctcgacc gcgtgatcca gatcgattcg ccatcgagcg tgtcgtcgtt cctgcagcgc
     6841 atgggcagaa ccggccgacg tgcaggcgcc ctgcggaact gcctgttcct gacgaccaat
     6901 gacgacgcct tcatgctggc cctgggtgtc accaagaagt ggtcggaagc atgggtcgaa
     6961 gcggcggtgc caccggccga gccctggccc atcctcgcgc agcaagcact ggttctggtg
     7021 ttggagcgcg gcgaggttcc aacgatggaa gtggtccagc tgcttcacgg atcgtttcca
     7081 gagctggcga ctgacgacat cacggcgttg gttgagcacc tggtggacaa gcagtttctt
     7141 gaccgctccg agggcgtggt cagggtcgga cctgagaccg agcgcgtgta tgcgcgcggc
     7201 cactaccgcg acctgctcgc ctcgttcagc ggatccatgc tgctgactgg tcggcatgga
     7261 tcgagcgagg tgggctacat cgatcccacc gttctgacgg gtgagcagga caaccggctc
     7321 ctgttgctgg ccggacgcag ctggcgtgtc accgaggtgg agtggtccaa acggatcgtg
     7381 tggctggagc cggcacggga aggtggcaag gcgcgctgga tgggcggagc gcgcagcctg
     7441 ggccgcgatg tttgccaggc cattagaacg gtgctcgcct caggcgcgcc accaatcgtc
     7501 accttgtcgc agcgtgcaag ggcggccctt agttcgctaa ccgacgagct gcccatgacg
     7561 ctgggagcgc acttcgtgat ggcgcgatct gatgctgcac ccgtacgtac ctggaccttt
     7621 gccggcacgc gcgccaaccg cacgtgggcg caccaggctt cggtcggtgg tcagaaggtt
     7681 cgattcgatg ccatgagtgt tcacgcgcca gcatcgctgc tggccgatgc agcgccgggg
     7741 cagttgacct tgacagacgc tgagattgcc accttcgccg agtcggtcaa gttcgccgaa
     7801 tgcgtgccac gaggcctgct cacccgcacg attgtcgcga ggaacttcga atcgccgatt
     7861 cgatgggaac cgacaccatg agcgccgctc gactctccca ggatgactac ccggaagtcg
     7921 gagctcgaag gcgcggtcac atcctcgtct gccatggaga cgacagtccg gatcagtgga
     7981 tcgcttggct agaacccatc cagtctcgtt tcaaggaact gcatgggctg tctgaggaat
     8041 atggtttcgc cttggacgcc ggtgaggaat cggggcgcct gggcagcgtt ggagaatttc
     8101 ttgagctgat cggcgtcgac atccggtcga acccgtggga aggctcgctc atcagcggcg
     8161 tctaccgatt cggttgggtc aaggttggta cggttcgggc agtgaggaag aaggccgcag
     8221 aactgctgaa ggcgcttcag cgccaggttg acagcgaggt tcggcgcaag atcctcgaag
     8281 atctcgccgg catcaagggc atcgaccaac tgatcgagcg tacggcagca gatcgtgatg
     8341 agctacgacg ccagatcctt gaatcgaagt cggccctttc caaggcccaa gaggaccttc
     8401 gactgacccg ggatcgattg cagcaactgg aggtctccga ctccaagcag atcgatgaat
     8461 cgtgccttcg catggctgga gcgatcctgc ggcgggcgcc ctgtcgaatg gttgccggtc
     8521 cgctggttct gtgggacgac aacgtcgtct atgcaatcac gtctcgtggc ggagtgacct
     8581 tgcccgccaa ctccacccca gccatcctcc tcggagatct ggaagccgcc gggtacccgg
     8641 ttgttcgcgt cgaggagaag accgtcatcg gtgggtggac aagtggcccg atctaccgaa
     8701 agggaacgcc acacatcacc ggatgggcaa gcgtgtggtc tgaccgggag cggaaggagc
     8761 gcgtgttttt ggtcgtcccg aaggacagcg agaggaaggt ccacgagatt caccaggcgc
     8821 ttgcgtcggg tttttcggga gcgtcgaagt gagagagaac gtcaccatgg aatgcggtca
     8881 ctcgcccgcc ccggggatgg attggaacgt caccgtttcc ttcgtgggca acgccaagat
     8941 gtaccgagta tggagtcggt cagatgagga tcagaaccgc gttctactga agcaggccgc
     9001 tgggccactg acctggcgcc gcatggttgc cgacatcaag gaactcgatg aaatcggcct
     9061 cggaggcgac ctgctcagcg acatccaggt cagcgggctg acgggttggc aatcgtccct
     9121 gctgaagatg gcctggtgca agtttgaggg gcctgcagag cgtcagctgg aatttctgct
     9181 cgaccttcca gacgaaacca tcgcccttct cgaggagaag ctcggagatc tcaccgatcc
     9241 tgatgtactc gcagcgctcg acgaagtgat cgaagccaag gaagaactgc agatctcgat
     9301 ggcgcaagtc gatcaagcga tcaacgaaac caccaaggca atgcgcaagg ggctggaccc
     9361 gtgggtgcga tcgatgtccg attggtttca ggcgcacgag gtgattgact ctgggccggg
     9421 ggtgtcgatg gaggtagctc gacgcagcac caccgctgaa acacttgcga tcgtgacgac
     9481 catgcaaagt ccgcttgctg tcgaggttgc acgagtgctg ctggtcaatt ggtcgaaggc
     9541 agagccgaag ccagtccaag aaggccgggt cgggggcatc cggttccggt caacgattcc
     9601 cttcctacag tggttggtgc agcggctgga acccggggca ctggcatcag tcactgccca
     9661 tgaagatggt gacgagctca atgcgatgga ggcgctggat gcccacatcg ctgccgagat
     9721 tcacaggctg cggacgtcaa gtggcggtgg cgcactcggt cacggtgtgc gtggcgggat
     9781 cgtcgccagc tgggaccggc tgcggcgaca agtcttcggg cccggctgat cgtcgctgtg
     9841 atgggcatgc cttcaacccg ggcgcaagcg atcgagcgcc aagcctcgga aatgcagtaa
     9901 aatttggtca catttggaat tgggtcgacg cccatgccaa cctgcctccc cgggcaaggt
     9961 ggaaacgcga aagcggatgt ggctcctact ggagcaacga agcgggtaca gcgtcggccc
    10021 tcccacaacg gagggctttt ttgttgttcg aatcaatcat cgacgctggc accgtgcagg
    10081 cctttcggga gaccgagtac agggtgcatg gcgacgaacc gttcaccctg aaagtgggcg
    10141 aaacctgctc ggcgttggcc gccgcgcaca agcgc
//
LOCUS       DAOORV010000121        10139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8254_length_10139_cov_9.097779, whole genome shotgun sequence.
ACCESSION   DAOORV010000121 DAOORV010000000
VERSION     DAOORV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_10139_cov_9.097779"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /gene="katG"
                     /locus_tag="PKV56_13985"
     CDS             complement(<1..1633)
                     /gene="katG"
                     /locus_tag="PKV56_13985"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOX68970.1"
                     /translation="MSNQAQCPFSGSARTQAIARSGPTNADWWPQQLNLKILHQHAAK
                     SDPMGESFNYAEEFRTLDLDGVVSDLHALMTDTQDWWPADWGHYGGLFVRMTWHAAGT
                     YRIADGRGGAGHGSQRFAPLNSWPDNGNLDKARRLLWPIKQKYGRKLSWADLIVLAGT
                     VAMDSMGFKTLGFAGGRADIWEPEEDINWGAETQWLATSDQPGSRYSGDRELANPLAA
                     VQMGLIYVNPEGPDGNPDPVASGRDVRETFARMAMDDTETVALVAGGHTFGKAHGAGD
                     PSLVGPAPEGAGIEEQGLGWTNAHGSGRGAHATTSGIEGAWKPNPTTWDMGYFDMLFG
                     YEWELTKSPAGAHQWTPRNCRPEHLIPDAHDPSRKSPPMMTTADLSLRFDPAYEKISR
                     RFHQNPSEFADAFARAWFKLTHRDMGPRARYLGKLVPNEVMIWQDPVPAVDHPMVNEQ
                     DISALKARLLASGLSISQLVNTAWASASTFRGSDKRGGANGARIRLAPQKDWEANQPA
                     ELSRVLTALEAIQKEFNGVQSGGKRVSLADLIVLGG"
     gene            complement(1829..2845)
                     /locus_tag="PKV56_13990"
     CDS             complement(1829..2845)
                     /locus_tag="PKV56_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOX68971.1"
                     /translation="MQAILTITIPFFALVLCGYLAARRGLLPVDAVPGLNAYVLFFAL
                     PCMLFRFGASMPFAQLIDPVLIGVYALCALAVVALTVALTWDRGTPVSGSTAATGVNL
                     KNAAFGALVASFPNTGFMGVPLLVALLGAPAAGPVIGTILVDLILTSTLCIALAQVRR
                     GTDEHRAADGSVTASVARSLRAALTNPLPWAIALGALSAAIEVDLPGPIATMVKLLAE
                     SATPVALFTIGAVLWRASLHSHTRTPLRHVLPVAAIKLLVHPLLVLGAGLSARALGAP
                     VPDFGLTVLVLAAALPSASNVSLLTERYGADNGRVARIIMASTALAFVSFSLLAWVMG
                     AGIP"
     gene            2953..4869
                     /gene="sppA"
                     /locus_tag="PKV56_13995"
     CDS             2953..4869
                     /gene="sppA"
                     /locus_tag="PKV56_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOX68972.1"
                     /translation="MSLRPLSLLRRGLAGVWWFIDAARRTALNLLFLLLIALLLIALL
                     RRESPVMQDNTALVLDLKGPLVEEQSGGVRDNALAQLRGNSIQSTRLRDVLAVLEAAA
                     TDPQIARVVLMLDDFQGAGLASLRDVATALQRFRSSGKQVVAWGSGYDQKQYYLAAHA
                     SEVLMHPLGAVYLDGYGRLRNYYLDALDKLGVGVSLVKVGTFKSAAEPFIANGPSPAA
                     KEADSVLYNGLWATYTEGVETARQLPAGSTQTLIDALPDRLAAVGGDAARLALEARQV
                     DALKTRDELRAMLIERGARDADSKSFRQVSFETYLARITPVVTGLATGSAIGVVVAEG
                     EIVDGQAPAGTVGGLSTATLVRKAREDDAIKAVVLRVNSPGGSVFGSELVRRELELTR
                     AAGKPVVVSMGSVAASGGYWISTASDEVMADAATVTGSIGVFALLPNASRAIDKLGVH
                     AEGVTTTWLGAAGDPRREPDPRFIGMLQTSVDHIYANFIGKVAQARQTTPEKIDDVAQ
                     GRVWTGLQAKERGLVDTIGGLSAAIRSAALRAKLLKEGDADPAASAAAGKVPDFRVVY
                     IDRDPGRFERLLEAFGGAAVSALARQFDLRLVPAGLPDSVTGPIRHDLGWLGALVDGR
                     KPFAAVTHCLCAAP"
     gene            4901..6973
                     /locus_tag="PKV56_14000"
     CDS             4901..6973
                     /locus_tag="PKV56_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX68973.1"
                     /translation="MTSAPASLNPAQLEAVHYLGGPCLVLAGAGSGKTRVITHKIARL
                     LEAGYAPGQIAAITFTNKAAQEMRERARQLIGPRAAKHLVVSTFHSLGVRILRQDGAR
                     LGLKPQFSIFDSDDVLGLLKEAGASTDNALARRWQWTISGWKNSGLNSEAAEAAATDD
                     DERVAARVMRRYEEQLAAYQAVDFDDLIGLPHKLLGQDEEVRSKWQDTLRYVLVDEYQ
                     DTNTIQYELLKSLVDHPDERRRGRFTAVGDDDQSIYGWRGATIDNLKRLPQEYPSLKV
                     IALEQNYRSTGNILRAANGVIANNPKLFEKKLWSDFGDGEPVRVVECDGEEHEAERAV
                     ARIQSLRGAGGANIDFKDFAILYRANHQARMLEKALRKANLPYKVSGGQSFFDRAEIR
                     DLCAWLRLLVNNDDDPAFLRAVSTPRRGIGTQTLASLGEFAGRWKTSLYEALFAESLS
                     AAVPARAIGSLHEFGRFVNDLEYRARHTSGAEDAKALLLGWLKDIGYEQHLHDGEDSE
                     KLAAARWSNVLDFVDWIAKRCGGEIENDGGMAIETERRSVLDVAQTISVIISLAERGD
                     EQNVVTLSTLHAAKGLEWPHVTLAGVNEGLLPFRSEDDEMTAERLEEERRLMYVGITR
                     ARSTLMVSTLRRRKKGRDTIQGVPSRFIGEMKLHEVQARVDPHEKLRALRAAAAERAA
                     QAAAGVKG"
     gene            7082..8440
                     /locus_tag="PKV56_14005"
     CDS             7082..8440
                     /locus_tag="PKV56_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOX68974.1"
                     /translation="MKSDTVIETIEVAALKIGMHIHLDGGWMSHPFPRSSFKISSLDQ
                     IATLRSLGLGCVRWSPQDSDLAPDTGRWGAGGPSAKVVKADEGQAAAQAAAAIADAAL
                     PSVDPAPAAPVRSEAADLAKRHRAALSAQREALAECERQFSEAARASKVVFDSVGAQP
                     AMARHGAQALTRQLIDQMIGAEGLCIRLLSEAAGDKVTSHAVNVSVISLIMGRTFGLL
                     DDELVDLGIGALLHDVGKLDVPERFRHRDDHHAAAEQKLYETHVAHGVAQARRMELSA
                     GASNVIAQHHELIDGSGFPLKLSMDRTTVMSRIVAIVNRYDNLCNPANPAKAITPHEA
                     VALMFAQGRSKYDPAILSGFIKMMGVYPAGSVVQLTDERYALVVGSNFSRPLKPRVLV
                     HDAKVPREEALILDLESAPELGIRRSLKPMALPERTLNYLSPRPRVSYFFEPTHEMEA
                     AA"
     gene            8437..>10139
                     /locus_tag="PKV56_14010"
     CDS             8437..>10139
                     /locus_tag="PKV56_14010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX68975.1"
                     /translation="MRILPNSPTPQLPVVAATPRRIRGEPAQALRERAGEGWLVAARA
                     LIDGLLDAVWLVDPDNLHIVAANRAAGELVGVEAGSLCGQDMLTLAATPEDMAFWQEA
                     ALGWTDSVESHTWVRRVDDSLVTVHRRVTRMVLPPEKAVFMVVMHDRSRERRAEDELA
                     DRLAELAATLESTQDGILVTDTSGRIRNFNQRFAAMWELPENLLSQRDDDAILSWMLR
                     RVVEPAEYMRRLAAIDGLPQTQSSDTITLIGGRVLERVTLPQCSRGQTIGRVYSFRDI
                     TERLEANRRIDLLSHTDGLTGLPNRRALLSRIEYALALAQREAVPFALLFLNLDRFKH
                     INDTLGHGMGDRVLMEVAERLLSTLRQVDTVARLGADEYVLLIHAADGPGAEGSARRV
                     LEAMLRPFTLDDLSFTVTCSVGIALYPGDGASADELIQAADTAMNEVKENGRAGYRFH
                     HARAESTTKLRTRMKLDHAMRQALAAGRFRLHYQPQVALESGEILGVEALIRWRDPEL
                     GDVPPAEFIPVAERSGFIVSIGAWVLRQAVQQAALWHRRGHRLLMSINVSALQFQQPD
                     FV"
BASE COUNT         1691 a         3395 c         3469 g         1584 t
ORIGIN      
        1 agccgcccag cacgatcagg tcggcgagcg agaccctctt gccgccggac tggacgccat
       61 tgaactcctt ctggatggct tccagcgcgg tgaggacccg cgacagttcg gcgggctggt
      121 tggcctccca atccttctgc ggcgccagcc gtatccgcgc gccattggct ccgccgcgct
      181 tgtcgctgcc gcggaacgtc gaggcggacg cccacgcggt gttgaccagc tgcgagatgg
      241 acaggccgga ggccagcaac ctggccttca gtgccgagat gtcctgctcg ttgaccatcg
      301 ggtgatcgac cgcgggcacc gggtcctgcc agatcatcac ttcgtttgga acgagcttgc
      361 cgaggtagcg ggcgcgcggc cccatgtcgc ggtgcgtcag cttgaaccag gcgcgggcga
      421 atgcgtcggc gaattccgac gggttctgat ggaagcgccg cgagatcttc tcgtaggccg
      481 ggtcgaagcg cagcgacaga tcggccgtgg tcatcatcgg cgggctcttc ctcgatggat
      541 cgtgcgcgtc cgggatcagg tgctcgggcc tgcagttcct cggcgtccac tggtgtgcgc
      601 cggccgggct cttggtcagt tcccactcgt agccgaacag catgtcgaag tagcccatgt
      661 cccaggtcgt cggattcggc ttccaggcgc cctcgatgcc gctggtcgtg gcgtgcgcac
      721 cccggccgct gccatgcgcg ttggtccagc ccaacccctg ctcttcaatg ccggcgcctt
      781 ccggcgcggg accgaccagc gaaggatctc cggcaccgtg ggccttgccg aaggtgtgac
      841 caccggccac cagggccacg gtttccgtgt cgtccatggc catgcgggca aaggtctcgc
      901 gcacgtcgcg gcccgatgcg accgggtcgg gattgccgtc cgggccttcc gggttcacgt
      961 agatcaggcc catctggact gcggccaggg ggttggcgag ttccctgtct cccgagtaac
     1021 ggctgccggg ttggtcgctg gtggccagcc actgggtttc ggcgccccag ttgatgtctt
     1081 cttcaggctc ccagatgtcg gcacggcctc cagcgaagcc gagggtcttg aagcccatcg
     1141 agtccatcgc gacggtgccg gccagcacga tcagatcggc ccacgacagc ttgcgtccgt
     1201 acttctgctt gatcggccac agcaggcggc gcgccttgtc gaggttgccg ttgtcgggcc
     1261 agctgttcaa gggggcgaag cgctgcgagc catggccggc accgccgcgc ccgtcggcga
     1321 tccggtaggt gcccgcggcg tgccaggtca tgcgcacgaa gagtccgccg tagtgccccc
     1381 agtcggcggg ccaccagtcc tgcgtgtccg tcatcaacgc gtggaggtcg ctgaccacgc
     1441 cgtccaggtc gagggtcctg aactcctcgg cgtaattgaa cgactcaccc atcgggtctg
     1501 atttggcagc gtgctgatgc aggatcttca ggttcagctg ctggggccac cagtcggcat
     1561 tggtcggacc gctgcgggcg atggcctgtg tccgggcgct gcccgagaac gggcactggg
     1621 cttggtttga catgcgtgtc tccttcgact ggttgcgatg tgctgcttgt ttggtgggac
     1681 gcacacgtcg tccgccaaaa aaatgtatcg agtcgagggg gtcttgcaaa tgcaattgat
     1741 aaaaaccctt cgatagcgtg gatcaattac cttgagtcgt gctgtgcgtc gagccacgac
     1801 ccatgcacga ctgtgccggg agcgcgtatc aggggatccc tgcacccatc acccaggcga
     1861 gcagcgagaa gctcacgaac gccagcgcgg tgctggccat gatgatgcgg gccacgcggc
     1921 cgttgtctgc gccgtagcgc tcggtcagca gcgacacgtt gctggcgctg ggcagtgccg
     1981 ccgccagcac cagcaccgtc aatccgaagt cgggaaccgg tgcacccagc gcacgcgccg
     2041 acagtcccgc gcccagcacc agcagcgggt gcaccagcag cttgatggcc gccacaggca
     2101 gcacgtggcg cagcggggtg cgcgtgtggc tgtgcaggct ggcgcgccac agcaccgcac
     2161 cgatggtgaa cagcgccacc ggcgtcgccg attcggcgag cagcttcacc atcgtcgcga
     2221 tgggtccggg cagatcgacc tcgatggctg ctgacagcgc accgagtgcg atggcccatg
     2281 gcagcgggtt cgtcagtgcg gcccgcagcg agcgcgccac cgatgcagtc accgaaccgt
     2341 cggccgcccg gtgttcatcg gtgccgcgcc gaacctgggc gagcgctatg cacagcgtgc
     2401 tggtcagtat cagatcgacg aggatggtgc cgatcacggg gccggcagcc ggcgcgccaa
     2461 gcagcgccac cagcagcggc acgcccatga agccggtgtt cggaaacgac gccaccagcg
     2521 cgccgaaggc ggcgttcttc aggttcacgc cggtcgctgc ggtgctgccc gacacgggcg
     2581 tgccccgatc ccaggtcagt gccacggtca gtgcgacgac tgcgagcgca cacagcgcat
     2641 acacgccgat cagcaccgga tcgatgagct gcgcgaacgg catgctggca ccgaaacgga
     2701 acagcatgca cggcagcgcg aagaacagca cataggcgtt cagccccggg accgcatcca
     2761 ccggcagcaa gccgcgccgc gcggccaggt agccgcacag caccagcgcg aagaacggga
     2821 tcgtgatggt gagaatcgcc tgcatgcgcg caggagtatc gcaacccggc gcgccggtca
     2881 ggggcaagcc atcgagcgct gcacggtgcg gcgcgtacag tctcgcgcgc cgaccttcaa
     2941 agtggagttt tcatgtccct gcgtcctctc tccctgctgc gacgcggact cgccggcgta
     3001 tggtggttca tcgacgctgc ccgtcgcacg gcgctgaacc tgctgttcct gctgctgatt
     3061 gcgctgttgc tgatcgccct gctccggcgc gagagcccgg tgatgcagga caacaccgcg
     3121 ctggtgctcg acctgaaggg cccgctggtc gaggagcaat cgggcggagt gcgcgacaac
     3181 gcgctcgcgc aattgcgcgg gaattcgatc cagagcaccc ggctgcgaga cgtgctggcg
     3241 gtactcgagg ccgcggcaac cgacccgcag atcgcccgcg tggtgctgat gctcgacgac
     3301 ttccaggggg cagggctggc cagcctgcgc gacgtggcga ccgcgctgca gcgtttcagg
     3361 tcgagcggca agcaggtggt tgcctggggc tcgggctacg accagaagca gtactacctc
     3421 gccgcccacg caagcgaagt gctgatgcat ccgctgggtg ccgtctacct cgatggctac
     3481 gggcggttgc gcaactatta cctcgacgca ctcgacaagc tcggcgtcgg cgtcagcctg
     3541 gtcaaggtgg gcacgttcaa gagcgcagca gaaccgttca tcgccaacgg tccgtcgccg
     3601 gcggcgaagg aggccgacag cgtgctctac aacggcctgt gggcgaccta caccgaaggc
     3661 gtcgagaccg cacgccagct gcctgccggc agcacccaga ccctgatcga tgcactgccc
     3721 gatcgcctcg cagcagtcgg cggcgatgca gccagactcg cgctcgaagc aaggcaggtc
     3781 gatgcgctga agacccgcga cgaactgcgc gcgatgctga tcgagcgtgg cgcgcgggat
     3841 gccgacagca agagcttccg gcaggtctcg ttcgagacct acctggcgcg catcacgccg
     3901 gtggtgacgg gtctcgccac cggcagcgcg atcggcgtcg tcgtggccga aggagagatc
     3961 gtcgatggac aggcgccggc cggcaccgtc ggcggcctgt cgactgcaac gctggtgcgc
     4021 aaggcgcgcg aggacgatgc gatcaaggcg gtggtgctgc gcgtcaactc tcccggcggc
     4081 agcgtgttcg gttccgaact cgtgcgccgc gaactcgagc tgacgcgcgc agccggcaag
     4141 ccggtggtgg tgtcgatggg cagcgtcgcc gcttccggtg gctactggat ctcgaccgcg
     4201 tccgacgagg tgatggccga tgccgccacc gtcaccggct cgatcggcgt cttcgcgctg
     4261 ttgccgaatg ccagccgcgc gatcgacaag ctcggcgtgc atgccgaagg cgtgaccacc
     4321 acctggctgg gtgccgcggg cgacccgcgg cgcgagcccg atccgcgctt catcggcatg
     4381 ctgcagacca gcgtcgatca catctatgcc aatttcatcg gcaaggtggc gcaggcccgc
     4441 cagaccacgc cggagaagat cgacgacgtg gcgcagggcc gtgtctggac cggcctgcag
     4501 gccaaggagc gcgggctcgt cgacacgatc ggcgggctct ctgccgcgat ccgctcggct
     4561 gcgctgcgcg ccaagctgct caaggagggc gatgcagacc cggctgcctc ggcggcggcg
     4621 ggcaaggtgc ctgactttcg cgtggtctac atcgaccgcg atccgggtcg gttcgagcgg
     4681 ctgctcgaag ccttcggcgg ggcagccgtc tctgcgctgg ccaggcaatt cgacctgagg
     4741 ctggtgccgg ccggtctgcc cgacagcgtg accgggccga tccgacacga cctgggctgg
     4801 cttggagccc tggtcgatgg ccgcaaaccc tttgccgccg tcacgcactg cctttgcgct
     4861 gctccgtgac ggcgcttccg atctgcatcc gacccgacgc gtgacctccg ctcctgccag
     4921 cctgaacccc gcccagctcg aggcggtgca ctacctcggc gggccgtgcc tggtgctggc
     4981 cggcgccggt tcgggcaaga cgcgtgtcat cacgcacaag atcgcacgcc tgctcgaagc
     5041 gggctacgcg ccggggcaga tcgccgccat caccttcacc aacaaggcgg cgcaggagat
     5101 gcgcgagcgc gccaggcagc tgatcggccc gcgcgcggcc aagcatctgg tcgtcagcac
     5161 cttccattcg ctgggcgtgc ggatcctgcg ccaggatggc gcgcggctcg gcctcaagcc
     5221 gcagttcagc atcttcgaca gcgacgacgt gctggggctg ttgaaggagg ccggtgccag
     5281 caccgacaac gcactcgccc ggcgctggca atggacgatc agcggctgga agaactcggg
     5341 cctcaacagc gaggcggccg aagcggcggc gaccgacgac gacgagcgcg ttgcagcgag
     5401 ggtgatgcgg cgctacgagg agcagctggc tgcctatcag gccgtcgatt tcgacgacct
     5461 gatcggcctg ccgcacaagc tgctcgggca ggatgaggaa gtgcgctcga agtggcagga
     5521 cacgctgcgc tacgtgctgg tcgacgaata ccaggacacc aacaccatcc agtacgagct
     5581 gctgaagagt ctggtcgacc acccggacga gcggcggcgt ggccggttca ccgcggtggg
     5641 tgacgatgac cagagcatct atggctggcg cggcgccacc atcgacaacc tcaagcgcct
     5701 tccgcaggag taccccagcc tgaaggtgat cgcgctggag cagaactacc gctcgaccgg
     5761 caacatcctg cgcgcggcga acggcgtgat cgcgaacaac ccgaagctgt tcgagaagaa
     5821 actctggagc gacttcggcg acggagagcc ggtgcgggtg gtcgagtgcg atggcgagga
     5881 gcacgaggcc gagcgcgcgg tggcacgcat ccagtcgctg cgaggtgcgg gaggcgccaa
     5941 catcgacttc aaggatttcg cgatcctgta ccgcgccaac caccaggccc ggatgctcga
     6001 gaaggcgctg cgcaaggcca acctgcccta caaggtgtcg ggcgggcaga gcttcttcga
     6061 ccgcgccgag atccgtgacc tgtgcgcctg gctgcgcctg ctcgtcaaca acgatgacga
     6121 cccggcattc ctgcgggccg tcagcacgcc caggcgcggc atcggcacgc agacgctggc
     6181 cagcctgggc gagttcgctg gccgctggaa gacgagcctc tatgaagcgc tgtttgccga
     6241 atcgctgagt gccgccgtgc cggcgcgagc gatcggctcg ctgcacgagt tcggccggtt
     6301 cgtgaacgat ctggaatacc gcgcacgcca tacctcgggc gccgaggatg cgaaggcgtt
     6361 gctgctgggc tggctcaagg acatcggcta cgaacagcac ctgcatgacg gcgaggacag
     6421 cgagaagctg gccgccgcgc gctggagcaa cgtgctcgac ttcgtcgact ggatcgccaa
     6481 gcgttgcggc ggtgagatcg agaacgacgg cggcatggcc atcgagaccg agcggcgcag
     6541 cgtgctcgac gtggcgcaga ccatctcggt gatcatcagt ctggccgagc gtggcgacga
     6601 acagaacgtc gtgacattga gcacgctgca cgccgccaag ggtctcgagt ggccgcacgt
     6661 cacactcgcc ggcgtaaacg agggattgct gccgttccgc tcagaagacg acgagatgac
     6721 ggcagaacgg ctggaagaag agcgccgcct gatgtacgtc ggcatcaccc gggcccgcag
     6781 cacgctgatg gtcagcacgt tgcggcgccg caagaagggc cgcgacacga tccagggcgt
     6841 gccgagccgc ttcatcggcg agatgaagct gcacgaggtg caggccaggg tcgatccgca
     6901 cgagaagctc cgggcattgc gggccgccgc cgccgaacgc gcggcgcagg cggcggcggg
     6961 cgtcaagggc tgaggccggg ccgacgcgcc tcggcgcgcg aaaccggcct gcgctcaagt
     7021 cggcaggcgg gcgtgccgaa aaggggccat acggcgttga tttctcaaca gcgccgctcc
     7081 catgaaatcc gacaccgtca tcgaaaccat cgaggttgcc gcgctgaaga tcggcatgca
     7141 catccacctg gacggtggat ggatgtcgca tccttttccg cgctcgagct tcaagatttc
     7201 cagcctggac cagatcgcca cgttgcgttc actgggcctg ggctgcgttc gctggagccc
     7261 gcaggacagc gatctggcgc cggacaccgg acgatggggc gcgggcggcc cgtcggccaa
     7321 ggtggtcaag gctgatgagg gccaggccgc tgcccaggcc gctgcggcga ttgcagatgc
     7381 ggcgttgccc tctgtcgatc ctgcgccggc tgcacccgtg cggtccgagg cggccgacct
     7441 ggcgaagcgg caccgtgcgg ccctgagcgc gcagcgcgaa gcgttggcgg agtgcgagcg
     7501 acagttcagc gaagccgcgc gtgcatccaa ggtggtgttc gacagcgtgg gcgcccagcc
     7561 cgccatggcg cgccacggtg ctcaggcgct cacgcggcaa ctgatcgacc agatgatcgg
     7621 agccgagggc ctctgcatcc ggctgctcag cgaagccgcc ggcgacaagg tcacctccca
     7681 cgccgtgaat gtcagcgtca tttcgctgat catgggcaga accttcggcc tgctcgacga
     7741 cgagctggtc gacctcggca tcggcgcctt gctgcacgac gtcggcaagc tcgacgtccc
     7801 cgagcgattt cgccatcgcg acgaccacca cgcggccgcc gagcagaagc tctacgagac
     7861 gcatgtcgcc cacggcgtcg cacaggcgcg gcgcatggaa ctcagcgccg gcgcgtccaa
     7921 cgtcatcgcc cagcaccatg aactgatcga cggcagcggt tttccgctga agctgtccat
     7981 ggatcgcacc acggtcatgt cgcgcatcgt tgccatcgtc aaccgctacg acaacctgtg
     8041 caacccggcc aatccggcca aggccatcac gccacacgag gcagtcgcgc tgatgttcgc
     8101 gcaggggcgc agcaagtacg accccgccat cctcagcggc ttcatcaaga tgatgggcgt
     8161 gtatccggca ggctcggtgg tgcagttgac ggatgaacgt tacgcgctgg tggtgggcag
     8221 caacttctcg cgcccgctca agccccgcgt cctggtgcac gacgccaagg tgccccgcga
     8281 ggaggcgttg atcctggacc tggagagtgc acccgagctc ggcatccgcc gcagcctgaa
     8341 gccgatggcg ctgcccgagc gcacgctcaa ctacctctct ccgcggcccc gcgtcagcta
     8401 tttcttcgag ccgacgcacg aaatggaagc tgccgcgtga ggatcctgcc caactcaccc
     8461 accccgcagc tgccggtcgt tgcggcgacg ccgcggcgca tccgtggcga gccggcgcag
     8521 gcgctgcgcg agcgggctgg tgagggttgg ctggtggcag cacgtgcgct gatcgacggc
     8581 ctgctggatg cggtctggct ggtcgacccc gacaacctgc acatcgttgc agcaaaccgg
     8641 gccgcgggcg agctggtcgg cgtcgaagcc ggcagcctct gcgggcagga catgctgacc
     8701 ctggccgcga ccccggaaga catggctttc tggcaggagg ctgcgctggg ctggaccgac
     8761 agtgtcgagt cgcacacctg ggtgcgccgc gtcgacgaca gcctggtcac cgtgcaccgg
     8821 cgtgtcacgc gcatggtgtt gccgcccgag aaggcggttt tcatggtcgt gatgcacgac
     8881 cgcagtcgcg aacgtcgcgc cgaggatgaa ctggccgatc gcctggccga gctggctgcc
     8941 acgctcgaat cgacgcagga cggcatcctg gtgaccgaca catcggggcg catcaggaat
     9001 ttcaaccagc ggttcgccgc catgtgggag ctgcccgaga acctgctgtc ccagcgggac
     9061 gacgatgcca tcctgtcgtg gatgctgcgg cgggtcgtcg agcctgccga gtacatgcgc
     9121 cggctcgccg cgatcgacgg cctgccgcag acgcagtcga gcgacaccat cacgctgatc
     9181 ggcggacgcg tgctcgagcg cgtcaccctg ccgcagtgca gtcgagggca gacgatcggc
     9241 cgggtctact cgttccggga catcacggaa cgccttgaag ccaaccggcg catcgacctg
     9301 ctgtcgcaca ccgacggact gaccggcctg cccaaccgcc gcgcgctcct cagccgcatc
     9361 gaatacgcgc tggccctggc gcagcgcgag gcggttcctt tcgcgctgct tttcctgaac
     9421 ctcgaccgct tcaagcacat caacgacacg ctgggccacg gcatgggcga ccgtgttctg
     9481 atggaagtgg ccgagcgcct gctgtccacg ctgcgccagg tcgacacggt ggctcgcctg
     9541 ggtgcggacg agtacgtgct gctcatccat gcggccgatg gtcccggcgc cgagggcagt
     9601 gcgcgccgcg tgctcgaagc catgctgcgc cccttcacgc tcgatgacct gagtttcacc
     9661 gtcacctgca gcgtcggcat tgcgctctat ccgggtgacg gggcgagtgc cgacgagctg
     9721 atccaggccg ccgacaccgc aatgaacgag gtcaaggaga acggacgcgc cggctaccgt
     9781 ttccaccacg cgcgtgccga gtccaccacg aagctgcgta cccgcatgaa gctcgaccat
     9841 gcgatgcgcc aggcgctcgc tgccgggcgt ttccgcctgc actaccagcc gcaggtggcg
     9901 ctggaaagcg gcgagatcct gggcgtcgag gcgctgattc gttggcgcga tcccgaactc
     9961 ggcgacgtgc cgccggcgga attcatcccg gtcgccgagc gcagcggctt catcgtctcc
    10021 atcggtgcgt gggtgctgcg ccaggcggtg cagcaagccg ctctgtggca ccggcgcgga
    10081 caccggttgc tgatgtccat caacgtgtcg gccctgcagt tccagcagcc cgacttcgt
//
LOCUS       DAOORV010000122         9916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8447_length_9916_cov_11.232634, whole genome shotgun sequence.
ACCESSION   DAOORV010000122 DAOORV010000000
VERSION     DAOORV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8447_length_9916_cov_11.232634"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKV56_14015"
     CDS             complement(<1..326)
                     /locus_tag="PKV56_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68976.1"
                     /translation="MTVLDLAQDTTKIQNGKQSICSKITSTGTIAISLVRPFGFAAGT
                     QALSFDKKKQSPVFPKGGSRGSKYLPVTPSKLAGFAVELAAYGVTHTDATGSVRRRLS
                     RTAPRSR"
     gene            325..621
                     /locus_tag="PKV56_14020"
     CDS             325..621
                     /locus_tag="PKV56_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68977.1"
                     /translation="MQLYGVIILDDFAVAHPECRGHLSAWRNEIEEARWKSLKHLQKR
                     HITAQVAQDGRVVFRLLGICMVDTKVRCELGIVLVQAAWVDGQPAKAATRALKK"
     gene            770..1876
                     /locus_tag="PKV56_14025"
     CDS             770..1876
                     /locus_tag="PKV56_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOX68978.1"
                     /translation="MVIGAYEAQNSQRSPVDAIEAINLRLDQQGLSKKDLVPYIGTMS
                     RVSDVLAGRRELTAAMMRKLHKGLGVPAASLIGDGDLDDEVDSELPDYDYTKFPLVEM
                     MERGCFGDIQYGLAKLRANAEELVAPLLRPLCGKVTQPALLRARLHQSGSRTMDEYAL
                     LFWRVLVLKKARKNPPRAPYKHGSITNTWLRDLAKLSAFDGGPRLAHEFLSKHGICLV
                     FERHFAKTYLDGAAMLDGDQPIVALTLRHDRLDNFWFALLHELVHVARHLRANHLFIA
                     DNLEDKTRKGQAEEDEADAGAQEALLPAKLWAKAAVTTTHSAHDALELAREAGVHPAI
                     VAGRLRHQTGNWRLLSNLVSDAGSVRQYFEEQLA"
     gene            2122..2436
                     /locus_tag="PKV56_14030"
     CDS             2122..2436
                     /locus_tag="PKV56_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68979.1"
                     /translation="MNIVETSSEELARPHGTLALRVRYLASPRPYLEPRAPLTETLAT
                     LKPKVLTFFGLMEGEVEGGRKEYAFSAHGVIQSNLAETLGQLAHGKHELELKLLEQFI
                     QG"
     gene            2443..2883
                     /locus_tag="PKV56_14035"
     CDS             2443..2883
                     /locus_tag="PKV56_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68980.1"
                     /translation="MATDFELALFAQQLEEVLELPEARRWSLERDESVPLQIFVVMHS
                     VSDPKEFYKARLRWRDLMKPPSLKFIDMTSDADNNPAAWPKCFGFRPGSLDACLPWTE
                     EGHGLHPEWANSAANAFPKVAAPVQFALLHLQSSLDNSYQGRGP"
     gene            2883..3395
                     /locus_tag="PKV56_14040"
     CDS             2883..3395
                     /locus_tag="PKV56_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68981.1"
                     /translation="MSEELNWALPANLLAVSIEVMRPHGRLGNEGLALWLGRVDGHAA
                     QVTHVVAPRGPGFRASPFQLKLSWNAMARLTDLADALGTYLVGQIHSHPGLFIDLSEV
                     DEKYGVRCQDYLSLVCPHYAQRPVSGIEECGVHMFDRGNYRRLGAAEVARRVRILPAK
                     VEVVDLEVPA"
     gene            3437..4975
                     /locus_tag="PKV56_14045"
     CDS             3437..4975
                     /locus_tag="PKV56_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68982.1"
                     /translation="MAQVLGGVALDEATVFSRPVVLTGEHTTLLTRNGQWCLLDSLRL
                     LSRIVGPLTVVLPAGLGEFEEEVQRLAKSLWTRGSVFVVSEGTPVAWSSATAILNVGA
                     RIDASLPWSSINSNGWVARVSSGPTSLPMDSEQSNPLAALMAASLGATEVFKRVYGIP
                     RDTAPLLDATQFSLFELSAAPTTLGPSLPPSLTLPDTVMVGGGAIGNGIALLLSQLAL
                     RGRLHIIDKQTFQRENLGTCVVLDDISWIGFSKATQLAGWLDRPDGLRCTGEEAFVED
                     ARSGTTVRSMAVDLVLNGLDDIGARHDAQRLWPSVLVDGGINAVGAAVTTQRLDHPEG
                     ACMICSFRAPKVDERTLQSRATGLSVESLSIGLNRQLTEQDVLQAHETQRDWLRSQMA
                     QGKTICAAITEAQTRALGMNTVVGFSPSVPFVATASAALVVAQALKALFFPSSEFAQR
                     FQMESLFIGPEASVGVLTHADLSCECVVHRRLIEKVAADRRRAGATAEALDAGARSAP
                     QRHG"
     gene            complement(4920..6665)
                     /locus_tag="PKV56_14050"
     CDS             complement(4920..6665)
                     /locus_tag="PKV56_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68983.1"
                     /translation="MNYLEAFPIPTHLLPPSLRALLSPVGLDPLEHVEVSAMRQSDDD
                     DVGSETLHLLMALVPEEDVEKLGVLNEAGDGVVAQSVPRLAKKGDSKEVVPSVSGYDY
                     VVASWGDGSFYTYSLAEKVWMTLGLTPRCLGNDQQRLVYDDLGLPEFGLADGEISAEF
                     HWSASRPVSWHMSNEYLRKYLWMRGAIGVRVFYYRTLLVDRPEIRGLMGGQSHASIDA
                     AGKWCDLDIQEAEGGLLLQMWASVAAVSCELCPEQSAEGLVWPGIDGPMTRDRANATL
                     HGGVVHLDDRFLERYEQSVFYDSTPSRRGDSNPSYRGQWSFTDCKRVGRNLVRVSIRE
                     LYKPKPDREIVHAHKFVVDPALVAGRDLNEEHIVSKTQRFVEQLVLLDEGLLGLAHAV
                     GVNVESNTLFGISRRELDANGWMSYPQLGRLAQVVPLDLAQGAFLSRCKSLHEIWQRI
                     PKGFLRSLLRRAGCPGEALKDLGSLKLLQGLLNIFEELDAHQENADALVSKDEPEGWQ
                     RDNQRMAPLFLNNDLRIADAHETLGGALQALQAMGFDIASLNQGYGRAIDFVFDAVTR
                     AFAALNEPLHRVLRR"
     gene            complement(6699..7658)
                     /locus_tag="PKV56_14055"
     CDS             complement(6699..7658)
                     /locus_tag="PKV56_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOX68984.1"
                     /translation="MNAITLPPIGKLERVPLREVWEHEAYDFTQWLQDNIEVLNDALG
                     LTLVNVDREQAAGAFSIDLVAEDEGGGTVIIENQLEKSNHDHLGKLITYLSAMGAKAA
                     IWIVSEPRPEHVAAIAWLNESNSAQFYMVKVEAVRIGASQPAALFTKIVGPSEEASSI
                     GQTKKDIAERHGIRNRWWTQLIEVSKKYNKLHVHITPSDYSWIGTSSGVRGLQLNYVV
                     TQDDGAVELYIDRGKDAEAENESIFDQLFAHKVEIETAFGGPLSWQRLEGKRACRIRY
                     TDPAGGYRSPEEQWPALHDALSRAMDRLERALRPHLKQLKLGA"
     gene            complement(7655..9040)
                     /locus_tag="PKV56_14060"
     CDS             complement(7655..9040)
                     /locus_tag="PKV56_14060"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOX68985.1"
                     /translation="MTELAQGWITAPLAAVVTIKTGPFGSALHKSDYLPTGIPIVNPM
                     HIRGGLIQPGSDARVGEAKLRELMEFRLAEGDVVLGRRGELGRCAVVGKVEAGWLCGT
                     GSLVLRPNGSVEPRYLQRFLSSPAVVARLEGDSVGSTMVNLNQGILKALEIPMPSLPE
                     QQRIADKLDALLARMDACRHRLDRVPGILERFRQAVLESALRGELSSEWRDSNSVSML
                     SWRSVTLADVADVGTGSTPLRSNAAFYSSTGTPWVTSAATGLAYVDTAGEFVTPAAVA
                     AHRLKTYPPGTLLVAMYGEGKTRGQVSELRIHATINQACAAVRVDESQASKAFVKLAL
                     WAQYEQMRALAEGGNQPNLNLSKIKGLAIRTPPIQEQAEIVRRVESLFALADAIDARY
                     TAARAQVERLTPALLAKAFRGELVPQDPNDEPASALLARLRNPAGHEEAPPARRPGRR
                     PARARAKESVI"
     gene            complement(9037..>9916)
                     /locus_tag="PKV56_14065"
     CDS             complement(9037..>9916)
                     /locus_tag="PKV56_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX68986.1"
                     /translation="TEKQQRKLRRSTFYGMEHVQDTHRLALMNLMLHGIDSDPEAGGI
                     RYGDTLGPDGQALGRLGATLILTNPPFGTKKGGGLPTRDDFTFPSSNKQFCFLQHIYR
                     ALKPGGRAAVVLPDNVLFESNVGTEIRRDLMDKCNLHTVLRLPTGIFYAPGVKTNVLF
                     FTRGETDKGNTKEVWVYDLRANMPAFGKRTMLGREHFAAFEVAFGADPLGAPASLKKR
                     QDEGEQGRWRRFKREAIAQRGDNLDIAWLKDESDESNGDQPDPLAVARDAMAELEGAL
                     EDLRAVVAELGEEVES"
BASE COUNT         1764 a         3199 c         3090 g         1863 t
ORIGIN      
        1 cggctacgtg gggcagtgcg gctgagccgc cgcctgacgc tcccggtcgc atccgtgtgg
       61 gtaacgccat aagctgcaag ttcgacagcg aaacccgcaa gttttgacgg cgtaaccggc
      121 aagtactttg agccccgtga acccccttta gggaacacgg ggctctgctt ttttttgtcg
      181 aacgacaaag cctgtgtccc tgcggcgaac ccaaaaggcc gaacaaggct tatggcgatg
      241 gttcccgtag aagtgatctt gctacagatc gattgttttc cattttgtat tttcgtggta
      301 tcctgagcca ggtcgagaac cgtcatgcag ctctacggcg tcatcatcct ggacgatttc
      361 gcagtggccc acccggagtg ccgcgggcac ctatccgcgt ggcgcaacga gatcgaggag
      421 gcgcgctgga agagcctgaa gcatctgcag aagcgccaca tcacggcgca ggtcgcccaa
      481 gacggccgtg tcgtcttccg gttgctgggc atctgcatgg ttgacacgaa ggtccggtgc
      541 gaactcggca tcgtcctcgt gcaagcggca tgggtcgatg gccagcccgc caaggctgcg
      601 acccgcgcgc tgaagaaata acccatacat aggcgactcc atgaccacac ccgtacgcat
      661 catcaggtcc aagcacgaat accaggcggc catcaaacgc ctgagcgact tgatgggcac
      721 ggagttcgag gccggctcgg acgcggagaa tgagttcgag ttgctgcgct tggtcatcgg
      781 cgcctacgag gcccagaact cgcagcgctc gccggtcgac gctatcgagg ccatcaacct
      841 gcgcctggat cagcaggggc tcagcaagaa ggaccttgtg ccctacatcg gcacgatgtc
      901 gcgcgtgtcc gatgtgctgg ccgggcgccg cgagttgact gccgcgatga tgcgcaagtt
      961 gcataagggt ctcggagtgc ccgcagcgtc tttgatcggc gacggcgatc tcgacgacga
     1021 ggtcgattcc gaactgccgg actacgacta cacgaagttc ccgttggtgg agatgatgga
     1081 gcgcggctgc ttcggcgata tccagtacgg cttggcgaag ctgcgcgcca acgcggaaga
     1141 gttggtcgct ccgctactgc gcccgttgtg cggcaaggtg acccagcccg cgctgttgcg
     1201 cgcccggctg caccagagcg gctcgcgcac gatggacgaa tacgccttgt tgttctggcg
     1261 tgtactcgtc ctcaagaagg ctcgcaagaa cccgccgcgc gcgccctaca agcacggcag
     1321 catcacgaac acatggctgc gtgacttggc caagctgtct gcgttcgacg gcggacctcg
     1381 gctggcgcac gagttcctaa gcaagcacgg catctgcctc gtcttcgaac ggcactttgc
     1441 caagacctac ttggacggcg ccgccatgct ggatggcgac cagcccatcg tcgcccttac
     1501 gcttcgccac gaccggctcg acaacttctg gttcgcgctg ctgcacgaac tcgtacacgt
     1561 cgcacggcac ttgcgcgcca atcatctctt catcgccgac aacctagaag acaagacgcg
     1621 aaagggccag gccgaggaag acgaggccga tgcgggcgcg caagaagccc ttctcccggc
     1681 caagctctgg gccaaggctg cggtgaccac tacgcactcg gctcacgacg cgctggagct
     1741 ggcgcgcgaa gctggtgttc atcccgccat cgttgcgggc cgacttcggc atcagaccgg
     1801 caactggcgc ctgctctcca acctagtcag cgacgcgggc tccgtccgcc agtacttcga
     1861 ggagcagctc gcatgaagct gaactcgtgc tgcgcgttgg cacgtattgc cgcgcagaca
     1921 acagactctg aaaggagttt tatggtccag aaatagttgc ggccgaatac ccggaggtac
     1981 tcgaccgcgg aggattcact gcaggcagct catccttgct tggtaggcga ggcgattgtc
     2041 tgtagcgacc tctgcgatgt caaggaacgt ccgcgccggt caggcagccg gctgaagtct
     2101 tcttgattga aagaggttca aatgaacatc gtcgaaacat cgagcgagga actcgctcgc
     2161 ccccacggaa cgctggctct gcgtgtccgt tacctggcgt cgccgcggcc ctacttggag
     2221 cctcgcgcgc cgctgaccga gacgctggca accctcaagc cgaaggtcct gaccttcttc
     2281 ggcctgatgg aaggcgaggt ggagggaggg cgcaaggaat atgcgttctc tgcccacggc
     2341 gtcatccaga gcaacctggc cgagaccttg ggtcagctgg cgcacgggaa gcacgaactg
     2401 gaactgaagc tgctcgagca gttcatccag gggtagtgcg ccatggcaac ggacttcgag
     2461 cttgcgctct tcgcgcagca gctggaagaa gtcctggagt tgccagaggc tcggcgctgg
     2521 agtctggagc gggacgaatc cgtcccgctc cagatcttcg tcgtcatgca ctcagtgagc
     2581 gaccccaagg agttctacaa ggctcgactt cggtggcggg accttatgaa gcccccgtct
     2641 ttgaagttca ttgacatgac cagcgacgcg gacaacaacc cggccgcgtg gcccaagtgc
     2701 ttcgggttcc gcccaggcag cctggacgct tgcctgccat ggaccgaaga gggccatggc
     2761 ctgcacccag agtgggcgaa ctccgccgcc aacgccttcc cgaaggtcgc ggccccggtg
     2821 cagtttgcgc tgctccactt gcagtcgtcg ctggataact cctaccaggg aaggggaccg
     2881 tgatgtcaga ggaactgaac tgggcgttgc cggccaacct gctcgcggtg tcgatagagg
     2941 tcatgcgccc gcacggtcgc ctggggaacg aagggctcgc gttgtggctc ggccgcgtag
     3001 acggccacgc tgcccaagtc acccacgtcg tcgcgccgcg cggcccgggc tttcgcgcgt
     3061 cgccgttcca gctgaagctg tcctggaatg cgatggcgcg gctgaccgac ctggcggacg
     3121 cgctggggac ttatctggtc ggccagatcc actcgcaccc tggactcttc atcgacttgt
     3181 cggaggtcga tgagaagtat ggcgtccgct gccaggacta cctctcgctc gtctgcccgc
     3241 actacgccca gcgtccggtg tcgggcatcg aagagtgcgg cgttcatatg tttgatcgtg
     3301 gcaactatcg acgcctcggc gccgccgaag tcgcgcgccg agtccggatc ttgcccgcga
     3361 aggtggaagt cgtggacctg gaggtgccgg catgaccgcc gcccacaacg acatcgcatc
     3421 gagccgacct atcggtgtgg cccaggtgct gggcggcgtc gcactagatg aagccacggt
     3481 gttctcgcgc ccggtcgtat tgaccggtga acacaccacc ctgctgacgc gaaatggcca
     3541 gtggtgcctg ctcgacagtc tgcggctact ctcgcggatc gtcgggccgc tgacggtcgt
     3601 cctgcccgcg gggctcgggg agttcgaaga ggaagtgcaa cgactcgcca agtccctctg
     3661 gaccaggggc agcgtgttcg tcgtgagcga gggcacgccg gtggcgtgga gttcggcgac
     3721 ggccatcctc aacgtcggcg cgcgcatcga cgcctcccta ccgtggagtt ccatcaactc
     3781 gaacggctgg gtggcgcgag tgtcctcggg cccgacctcg ctgccgatgg atagcgagca
     3841 gagcaatccg ctggcggcgc tgatggccgc atcccttggt gccaccgagg tcttcaagcg
     3901 cgtctatggc atcccgcgcg acacggcgcc gctgctggat gcgacgcagt tttcgctgtt
     3961 cgagttgagc gccgcgccaa cgacgctcgg cccttcactt cccccttcgc tcacgctgcc
     4021 tgacactgtg atggtcggtg gcggcgccat tggaaacggc atcgccttgc tgctatcgca
     4081 actcgccctc cgagggcgcc tgcacatcat cgacaagcag accttccagc gagagaacct
     4141 tggtacctgc gtcgtgcttg atgacatctc ctggatcggg ttttcgaagg cgacgcaact
     4201 ggctggttgg ctcgaccgac ccgacgggct tcgctgcacc ggcgaagaag cgttcgtgga
     4261 agacgcgcga tcgggtacca ccgtgcgctc gatggcggtg gacctggtct tgaatggcct
     4321 ggatgacatc ggcgctcggc acgacgccca acggctctgg ccgtccgtgt tggtggacgg
     4381 cggcatcaac gccgtcgggg ccgcggtgac cacgcagcga ctggaccacc ccgagggtgc
     4441 ctgcatgatc tgctccttcc gcgcgccaaa ggtcgacgag cggacgctgc agtcgcgggc
     4501 gactggcctc agcgtggaat cgctcagcat cggcttgaac cgccagttga cagagcagga
     4561 cgtccttcaa gcgcacgaaa cgcagcgcga ctggctgcgg tcgcaaatgg cgcaaggcaa
     4621 gaccatttgc gccgccatca ccgaggcgca gacgcgcgcg ctggggatga acaccgtagt
     4681 tggattcagt ccatcggtgc cgttcgtcgc caccgcatcg gctgcgctag tggtggctca
     4741 ggcactgaag gcgctcttct ttccgagcag cgagttcgca cagcgctttc agatggagag
     4801 cctctttatc ggacctgaag catcggtggg agttctaact cacgctgacc tgagttgcga
     4861 atgcgtggtt catcgtcgcc taatcgaaaa ggtcgctgcc gatcggcgcc gagctggggc
     4921 taccgccgaa gcactcgatg caggggctcg ttcagcgccg caaaggcacg ggtgacagcg
     4981 tcgaacacga agtcaatcgc acgtccgtag ccctggttca ggctggcaat atcgaacccc
     5041 atcgcctgca gcgcttgtag cgcaccgcct agcgtctcat gtgcgtcagc gattctgagg
     5101 tcgttgttca ggaatagcgg cgccatgcgc tggttgtccc gctgccagcc ttcgggctca
     5161 tccttggaca ccagcgcgtc agcattctcc tggtgggcat ccaactcctc gaagatgttc
     5221 aggagcccct gcagcagctt caggctgccc agatccttca gtgcctcgcc gggacagccc
     5281 gcgcgtcgaa gcagcgagcg caagaaccct ttgggaatcc gctgccagat ctcgtgcagg
     5341 ctcttgcatc gggacaagaa ggcgccttgc gcgaggtcga gcggcacgac ctgcgccaga
     5401 cgcccgagtt gtgggtagct catccagccg ttggcgtcca actctctacg cgaaatgcca
     5461 aacagcgtat tgctttcaac gttcactccg acggcatgcg ccaatccgag cagaccttca
     5521 tcaagaagca ccaactgctc gacgaagcgc tgcgtctttg agacgatgtg ctcctcgttg
     5581 aggtcgcgtc cggccacaag cgctgggtcc accacgaact tgtgtgcatg gacgatctcg
     5641 cgatcaggct tcggtttgta caactccctg atcgaaaccc gaactaggtt gcgcccaacg
     5701 cgcttgcagt ccgtaaacga ccattgcccg cgataggatg ggttgctgtc tcccctcctg
     5761 ctaggcgtcg agtcgtagaa gacgctttgc tcgtaccgct ccaggaatcg gtcgtccagg
     5821 tgaaccacgc cgccgtgcaa cgtcgcattc gcgcgatccc gcgtcatggg cccgtcgatt
     5881 cccggccaaa caaggccctc cgcgctctgc tcggggcaca actcgcatga cactgcagcc
     5941 accgaagccc acatctgaag aaggagccca ccttcggctt cctggatatc caggtcgcac
     6001 cacttgccgg cggcatcaat ggaggcatgg gactgaccgc ccatgaggcc tcggatttca
     6061 ggcctgtcca cgagcagcgt gcggtagtag aacacccgca caccaatcgc tcctcgcatc
     6121 cacaggtact tgcgcaggta ctcgttggac atgtgccaac tgacaggccg ggacgcgctc
     6181 cagtggaact ccgcagagat ttcgccgtca gcgagtccga actccggcag gccgaggtcg
     6241 tcatagacca gccgctgttg gtcatttccg aggcagcgag gtgtaagccc cagagtcatc
     6301 cataccttct ctgccaagct gtacgtatag aacgagccgt ccccccatga ggcgacgacg
     6361 tagtcgtagc cggacacgga agggacgacc tccttggagt cgcccttctt tgcgagccta
     6421 gggactgact gggcgacgac accatcgccc gcctcattca ggacgccgag cttctcaaca
     6481 tcctcttccg gaactagcgc catcagcaag tggagcgtct cggagccgac gtcgtcgtca
     6541 tcgctctgac gcatggccga aacttccacg tgctcaagcg ggtcaagccc gaccggcgac
     6601 aacagcgcgc gaagactcgg cggaagcaga tgcgtcggga tcggaaacgc ttccagatag
     6661 ttcatcgatg ctgcaccaat aggaagctgt ggccgacact aagcacccag cttcagctgc
     6721 ttcagatgcg gccgcagcgc gcgctcaagc cgatccatcg cgcgactcaa ggcgtcgtgc
     6781 agcgcgggcc attgctcctc cggcgagcgg taaccgcccg cagggtctgt gtatcggatg
     6841 cggcacgctc gtttgccttc cagacgctgc cagctcagcg ggccgccgaa tgccgtttcg
     6901 atctcgacct tgtgggcgaa caactggtcg aagatcgact cgttctcggc ctcggcgtcc
     6961 ttgccccggt cgatgtacag ctccaccgcg ccgtcgtcct gtgtcaccac gtagttcagc
     7021 tgcaggccgc gcacgccgga gcttgtgccg atccagctgt agtcgctggg cgtgatgtgc
     7081 acgtgcagct tgttgtactt cttcgagacc tcgatcagct gggtccacca gcggttgcgg
     7141 atgccgtggc gctcggcgat gtccttcttg gtctggccga tgctgctggc ctcttccgac
     7201 ggaccgacga tcttggtgaa cagcgccgcc ggctgcgagg cgccgatccg caccgcctcc
     7261 accttgacca tgtagaactg cgcgctgttg ctctcgttga gccaggcgat ggcggccacg
     7321 tgctcgggcc gcggctcgga gacgatccag atcgccgcct tggcgcccat cgcgctgagg
     7381 taggtgatga gcttgccgag gtggtcgtgg ttgctcttct cgagctggtt ctcgataatg
     7441 accgtgccgc caccttcgtc ctcggccacg aggtcgatgc tgaaggcccc ggcggcctgc
     7501 tcgcggtcga cgttcaccag cgtcaggccc agagcgtcgt tgagcacctc aatgttgtcc
     7561 tgcagccatt gcgtgaagtc gtaggcctcg tgctcccaga cttcgcgcag cggcacacgt
     7621 tcgagcttgc cgatgggcgg cagggtgatg gcgttcatat gacggactcc ttggctctcg
     7681 ccctggccgg gcggcggccc ggcctgcggg ccggtggtgc ttcctcgtgc ccagccggat
     7741 tgcgcaggcg tgccagcagc gcgctcgcgg gttcgtcatt cgggtcctgc ggcacgagct
     7801 cgccgcgaaa cgccttggcc agcagcgccg gcgtcaggcg ctcgacctgg gcgcgggcgg
     7861 cggtgtagcg ggcgtcgatg gcgtcggcga gcgcgaagag ggattcgaca cgtcggacga
     7921 tttcggcttg ctcctgtatg ggtggagtac gaatcgccaa gcccttgatc ttggagaggt
     7981 tcaggttcgg ctggttgccg ccttcggcca atgcgcgcat ctgctcgtac tgcgcccaca
     8041 acgcgagctt cacaaaggcc tttgacgcct gactttcgtc cacgcggaca gcggcgcaag
     8101 cctgattgat ggtggcatga atgcgtagct cgctgacctg gccccgagtc tttccttccc
     8161 cgtacatggc gaccagcaag gtacctggtg ggtaggtctt caagcgatgt gctgccacgg
     8221 ccgcaggagt tacaaactcg cctgctgtat cgacgtaagc gagcccggtc gctgcgctcg
     8281 tgacccacgg cgttccggta gatgagtaga aggcggcgtt tgaacgaaga ggcgtcgacc
     8341 ctgtccctac gtcggccacg tccgccaacg tgaccgaacg ccaactcagc atgctcactg
     8401 agttcgagtc ccgccattcc gacgagagtt cccctcgtag cgcggactcg agcacagcct
     8461 gccgaaagcg ctcgaggatg cctggcacgc ggtccagccg gtggcggcag gcgtccatgc
     8521 gggcgagcag ggcatcgagc ttgtcggcga tgcgctgctg ctccggcaac gaaggcatgg
     8581 gaatctcgag cgctttcaat atgccctggt tcagattcac catggtggat ccgaccgaat
     8641 cgccctctaa gcgcgccaca acagcagggc tggacagaaa gcgctgcaga taccgaggct
     8701 caacgcttcc gtttggccgc aacacgaggc ttccagtccc gcacagccag ccagcttcga
     8761 ccttcccgac cactgcgcag cgtcccaact caccgcgacg cccaagcaca acgtcacctt
     8821 cggcgaggcg aaactccatg agctctctta gcttggcctc tccgacccgc gcatcggatc
     8881 ctggttgtat gagtccgccg cggatgtgca tcggattgac gatcgggatt cccgtgggca
     8941 gatagtcaga cttgtggagc gcgctgccga atggaccggt cttgatggtc acaaccgcag
     9001 caagtggcgc cgtgatccat ccttgcgcga gctcggtcaa gattccacct cttcaccgag
     9061 ttctgcgacg acggcacgca gatcttccag cgcgccttcc agttcggcca tcgcgtcacg
     9121 tgcaacggcc agcgggtcag gctgatcgcc gttgctttca tcgctctcat ccttcaacca
     9181 tgcaatgtcg aggttgtctc cccgctgcgc aatcgcctcc cgcttgaacc gccgccaccg
     9241 cccctgctcg ccctcgtcct gacgcttctt caggctcgct ggcgcaccga gcggatcggc
     9301 gccgaacgcg acctcgaacg ccgcgaagtg ctcgcgcccg agcatcgtcc gcttgccgaa
     9361 ggccggcatg ttggcgcgca ggtcgtagac ccacacctcc ttcgtattgc ccttgtccgt
     9421 ttcgccgcgg gtgaagaaga gcacgttggt cttcacgccc ggtgcataga agatgccggt
     9481 gggcaggcgc aggacggtgt gcaggttgca cttgtccatc aagtcacgcc ggatttcggt
     9541 gccgacattg ctctcgaaca gcacgttgtc gggtagcacc acggccgccc tgccgcctgg
     9601 cttcaacgca cgatagatgt gctgcaagaa gcagaactgc ttgttgctgg aggggaaggt
     9661 gaagtcgtcg cgcgtcggca ggccgccgcc cttcttggtg ccgaaaggcg ggttcgtgag
     9721 gatcagcgtc gcgcccagcc tgcccaatgc ctgaccgtcc ggtcccagcg tatcgccgta
     9781 gcggatgccg ccggcctcgg ggtcgctgtc gatgccgtgc agcatcaggt tcatcagcgc
     9841 gaggcgatgc gtgtcctgca catgctccat gccgtagaag gtgctgcgac gcagcttgcg
     9901 ctgctgcttc tcggta
//
LOCUS       DAOORV010000123         9773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8633_length_9773_cov_9.500926, whole genome shotgun sequence.
ACCESSION   DAOORV010000123 DAOORV010000000
VERSION     DAOORV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_9773_cov_9.500926"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKV56_14070"
     CDS             complement(<1..382)
                     /locus_tag="PKV56_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68987.1"
                     /translation="MKKRLFLAAAAVISLCGAGMAQARDLNWAVSIGDPMVGAVISNG
                     PVYGYAPRVHAPPPVVVLPAPRVVYERPYAYAPVPYGYATYGYVPHRHGRHDDWRWSR
                     RYEERNRHGWRDHDRRDDRGGRDDY"
     gene            661..2508
                     /locus_tag="PKV56_14075"
     CDS             661..2508
                     /locus_tag="PKV56_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOX68988.1"
                     /translation="MTAPAHPTSPDDTKPSNFLRQVIERDLAQGTYASRTFAGTPGDA
                     AHHAAGPLDPARIRTRFPPEPNGYLHIGHAKSICLNFGLARDYGGVCHMRFDDTNPEK
                     EEQEYVDSILDAVKWLGFGWETPDPLNAGATRSHLYYASNYFDFMYRAAEALIGAGHA
                     YVDEQTADQMRANRGDFNTPGTDSPFRSRTPAENLARFREMRDGQLADGAAVLRAKID
                     MASPNINLRDPAIYRIKRATHHNTGDTWCIYPMYTYAHPIEDALENITHSICTLEFED
                     QRPFYDWLLDMLCTLGLTKQPRPHQYEFARLNITYIVTSKRKLKQLVDEGHVQGWDDP
                     RMPTIVGLRRRGYTAESLQLLAERSGVSKAGGWLDYSSIDIALRDDLDPKAARACAVL
                     DPLKLKLTNFAEVFGSESHREPCHAPVHPAHPERGQRDFVLTNEVWIEREDFMEVPSK
                     GFFRLSPPRIDAAGVAQPGSRSRLKYGYVIECTGCEKDAAGQVTAVLATLVPDTKSGT
                     PGADAVKVKGVITWVSVATGVAAEVRLYDRLFTEAQPDAGGRDFLTVLNPQSRKIVTA
                     YLEPGLEKAGADDRFQFERHGYFVADRKDHTAERPVFNRTTTLKDTWAK"
     gene            complement(2567..3433)
                     /locus_tag="PKV56_14080"
     CDS             complement(2567..3433)
                     /locus_tag="PKV56_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68989.1"
                     /translation="MLFASMPHRGWLLLCCLLAGPAGSQTLPTVKIGNEVKATEGILV
                     GQQEGDAACYLTLRDDAGRQFTERADFEVCVTSGKLTGKRVRLAYTPGRVMHEDCQGD
                     PSCRKSRTVPLVSSLRPAEPPATVVAPQAAPAGAASFCSAAETTVFTCRIGARRVSVC
                     ADPASTAQRGYVQYRFGKADGSVPLELEIPSRRIEPSAAAAGASVVFAAGGSAWLRFF
                     NGDHAYVVYTGIGKWGPRGAIQEKEGLVVERRGKQIASLKCSQAAVSILGPEWFEQVG
                     ITARDGDFELPD"
     gene            complement(3478..4017)
                     /locus_tag="PKV56_14085"
     CDS             complement(3478..4017)
                     /locus_tag="PKV56_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX68990.1"
                     /translation="MGSACWVNALTLASLLAWGPAAAAEVDTSQARQVYAEVNAKLPH
                     MTRVSFRARRPDVEYVSEVRAWSDAEGVRKLEVTDRDDSGDVVGEYFFAGRQLVFVYE
                     AIRGYTESGRQVTRVEDRQYFREGRMIRWLGGLEKVEQLRETPDFLAAQLSRLEAADF
                     YRKAAERAAATPAAGPSTR"
     gene            complement(4017..4505)
                     /gene="queF"
                     /locus_tag="PKV56_14090"
     CDS             complement(4017..4505)
                     /gene="queF"
                     /locus_tag="PKV56_14090"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOX68991.1"
                     /translation="MARTPKKSSVPPEPAKQRVAPINPPTAPSKELHVFPNPSPERDY
                     VIQFQIPEFTCLCPLTGQPDYAHFVIDMVADDLCVELKSLKMYMWSFRDEGAFHEKVT
                     NDILTKIVAVTQPRYARITARWYVRGGIYTNVVADHRKKGWKPQTPVVLPSHPGETGL
                     LG"
     gene            complement(4531..5166)
                     /gene="queE"
                     /locus_tag="PKV56_14095"
     CDS             complement(4531..5166)
                     /gene="queE"
                     /locus_tag="PKV56_14095"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HOX68992.1"
                     /translation="MTYAVKEVYYTLQGEGGQSGRAAVFCRFAGCNLWSGREADRAEA
                     VCRFCDTEFVGTDGPGGGKFDTPQALAAHIAQRWPADRPGRPLVVCTGGEPLLQLDEP
                     LIDALHALGFEVAVETNGTQQAPAGLDWICVSPKAGAELVLTHGHELKLVFPQPGAMP
                     EHFEGLDFGLFFLQPMDGLEQRRNTRLAVDYCLVHPQWRLSVQTHKTLGIP"
     gene            complement(5183..6271)
                     /locus_tag="PKV56_14100"
     CDS             complement(5183..6271)
                     /locus_tag="PKV56_14100"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOX68993.1"
                     /translation="MTASVRTLFSASAAFQAARQLDAVPVGHPSGRLHGHGFVAKVRC
                     ELPAGWARFQGGEVDRLRDELQACVGVLDHRLLNEHLPQPSDANLAAWVRDRLALPGR
                     VDVGVQSTAQCGADLDATGRTRLWRRFAFHSAHRLPHVPLGHKCGTMHGHGFEAIVHA
                     SDSGSGLSHDDLDAIWAPIHFQLNYACLNELPGLENPTSELLASWLWARIAPQLPGLS
                     TITVFETGSCGAVFDGQRHRIWKQMTLDGALQLKHAPEGSGLRRLHGHTYTLRLHLSA
                     PLDDWLGWAFDFGDVKAAFDPIFQMLDHRPIHEIADLADCDTASIAHWVLERARLQLP
                     QVDRVDLEETAGCGAIVSTGSATLSGPM"
     gene            complement(6274..6990)
                     /gene="queC"
                     /locus_tag="PKV56_14105"
     CDS             complement(6274..6990)
                     /gene="queC"
                     /locus_tag="PKV56_14105"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOX68994.1"
                     /translation="MTDSVSGRPPAVVLLSGGLDSATVLAIARERGFEPCALSFRYGQ
                     RHVHELTAADVVARSQGVARHVVAEIDLRQFGGSALTADIDVPKGRTAGEMAHGIPIT
                     YVPARNTVFLSFALAWAETLGSSDIFIGVNALDYSGYPDCRPEYIAAFEAMANLATQA
                     GVEGTQRLKIHAPLIDLSKEQIIREGLRLGVDYGQTSSCYDPGVDGRPCGGCDSCLLR
                     AKGFAEAGVRDPLLARFGIV"
     gene            7102..9312
                     /locus_tag="PKV56_14110"
     CDS             7102..9312
                     /locus_tag="PKV56_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOX68995.1"
                     /translation="MSDTPNLPNPRHASRVPSHIGKAQFERIRQAAYRLPQVVKPGDY
                     IAERSAGFEEHLDKLGLVLLQKPVAGARPSRWIAPDLMDSALTHLDAVGNGPAVRPLM
                     LERTAALEAMGVTRDAGVWTRLISHALTLSEAGWKVPVLFRVPLNDAAVWDFYRTGDS
                     AALQAAIGPPTPYPNAAELVTHLDDLVARSRALKPNHLESLLPRLQSECATPQALADL
                     KAALMRAQDRWEHRASELDTIEGLNSELAFQGYPDTFEKARRLQRSVTLYVGPPNSGK
                     THAAFERLAQAHDGAYLAPLRLLALEGRDRLVGRGVPCSLLTGEENVPADGARVVSST
                     IEMVGTNKPIDVAVIDEAQMIFDVSRGWAWTQAIVAVPANEVIIICSAYAVPAIENLL
                     GLCGERCTVRHFERKQHVELLPHPIATSALQLGDAVVAFSRRDVLSLRDRIAADGHPV
                     SVIYGALPPEVRRREAERFASGESHILVATDAIGMGLNLPIRRVLFSTMTKFDGVADR
                     PLNESEVHQIAGRAGRFGIHEEGFVGVLKDAEPQAARMLKELLPKTPRAPRDFKAPVA
                     PNGWHVDTISSRLHKTKLREVLGVFMEQLKLDDAHFAVAELDQMLDLAEQLDRCAPKL
                     SLKERFIYAQAPVDTRTDSQVQEFLDWTSSHALTGKAGSPWFLKDVDGHSRLDRMEQA
                     LRACTLWLWLDLRFPGVYGHIDAVLELRSQLNDGIERQLKGKRPLAQVRQRARR"
     gene            9494..>9773
                     /locus_tag="PKV56_14115"
     CDS             9494..>9773
                     /locus_tag="PKV56_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOX68996.1"
                     /translation="MKTALLFAVVSTAAITLLPVATAQAQVSQAEAQINALEGLFGKH
                     AGVRRSQAKGVCASGYFVGNAVGRSLSSAAVFNGDRVPAGQRCASTSNR"
BASE COUNT         1661 a         3452 c         3229 g         1431 t
ORIGIN      
        1 ggtagtcgtc gcgccctccg cggtcatcgc ggcggtcgtg gtctctccat ccgtggcgat
       61 tgcgttcctc gtatcgacgg ctccaccgcc agtcgtcatg acggccgtgg cgatgcggga
      121 cgtatccgta ggtcgcgtag ccatagggca ccggcgcata ggcgtaaggc cgctcgtaga
      181 cgacccgcgg agccggtagg accaccacgg gcggaggtgc atggacccgt ggcgcgtagc
      241 cgtacaccgg gccgttcgag atcacggcgc ccaccatcgg gtcgccaatc gacaccgccc
      301 agttcagatc gcgcgcttga gccatgcctg cgccgcagag agagatcacc gcagcagcgg
      361 ccaggaacag acgtttcttc atctctagtc tcctcgttga ggtcgcgctt cacgatccag
      421 tgtcaccggg cctgaagccg ccgaaccgca caccggactg gtgtgcccgt ccacaacgcg
      481 gcccgaggac acgctcgttg accctcgaag cgaaagcgga ggtaaagaca tgtgaagcca
      541 gcgtgaagct gctgcctgcg ccggtccggt gcccgaggcc cgggagggat aattgccgtc
      601 gcctcgaacg aggcgacttc ccccctttcc gctgcccccg cgccagccgc gcacgcctcc
      661 atgaccgcac ctgcccaccc gacctcgccc gacgacacga agccgagcaa tttccttcga
      721 caggtgatcg agcgcgatct ggcccagggc acgtacgcca gccgcacctt tgccggcacg
      781 ccgggcgacg cagcgcacca cgccgccggt ccgctcgacc ccgcgcgcat ccgcacccgc
      841 ttcccgcccg agccgaacgg ctacctgcac atcggccacg ccaagagcat ctgcctgaac
      901 ttcgggctgg cgcgcgacta cggcggcgtg tgccacatgc gtttcgacga caccaacccg
      961 gaaaaagaag agcaggaata cgtcgactcg atcctcgatg cggtgaagtg gctgggcttc
     1021 ggctgggaga cgcctgaccc gctgaacgcc ggcgcgaccc gcagccatct ctactacgcc
     1081 agcaactact tcgacttcat gtaccgcgcg gccgaggcgc tgatcggcgc cggccatgcc
     1141 tacgtcgacg agcagaccgc tgaccagatg cgcgcgaacc gcggcgactt caacactcca
     1201 ggcaccgaca gcccgttccg cagccgcacg ccagctgaaa acctggcgcg attccgcgag
     1261 atgcgcgatg gccaactggc cgacggcgct gccgtgctgc gcgcgaagat cgacatggct
     1321 tcgcccaaca tcaacctgcg cgacccggcc atctaccgca tcaagcgcgc cacgcaccac
     1381 aacaccggcg acacctggtg catctatccg atgtacacct acgcgcaccc gatcgaggac
     1441 gcgctcgaga acatcaccca cagcatctgc acgctcgagt tcgaggacca gcgccccttc
     1501 tacgactggc tgctggacat gctgtgcacg ctgggcctga cgaaacagcc acggccgcac
     1561 cagtacgagt tcgcgcggct caacatcacc tacatcgtca ccagcaagcg aaagctcaag
     1621 cagctcgtag acgaaggcca cgtgcagggt tgggacgacc cgcgcatgcc gaccatcgtc
     1681 gggctgcgcc gccgcggcta caccgccgag agcctgcaac tgctggctga gcgcagcggc
     1741 gtcagcaagg ccggcggctg gctcgactac tcgtcgatcg acatcgcgct gcgcgacgac
     1801 ctcgacccga aggccgcgcg cgcctgcgcg gtgcttgatc cgctgaagct gaagctgacc
     1861 aatttcgccg aggtgttcgg cagcgagtcg catcgcgaac cgtgccatgc cccggtgcac
     1921 ccggcgcatc ccgaacgggg ccagcgcgac ttcgtgctga cgaacgaggt gtggatcgag
     1981 cgcgaggact tcatggaagt gccgagcaag ggcttcttcc gcctcagccc gccccgcatc
     2041 gacgcggccg gtgtcgcgca gcccggcagc cgatcacggc tgaagtacgg ctacgtgatc
     2101 gagtgcaccg gttgcgagaa ggacgccgca ggacaggtca cggcggtgct ggccacgctg
     2161 gtgcccgata ccaagagcgg cacgccaggg gccgatgccg tgaaggtcaa gggcgtgatc
     2221 acctgggtca gcgtggccac cggcgtcgcc gccgaagtgc ggctctacga ccgcctgttc
     2281 accgaagccc agcccgatgc aggtggacgt gatttcctga ccgtgctgaa tccccagagc
     2341 aggaagatcg tcaccgccta cctcgaaccg ggactggaga aggccggtgc cgacgaccgc
     2401 ttccagttcg agcgccacgg ctacttcgtc gccgaccgca aggaccacac ggcagagcga
     2461 ccggtcttca accggacaac gacgctgaag gacacctggg ccaagtgaga acggcgggtc
     2521 tggcgactcg ctgcaagccg ggcgttcggc gccggccgct ccagcctcaa tcgggcagtt
     2581 cgaaatcgcc gtcgcgcgcg gtgatgccga cctgctcgaa ccattcgggc ccgaggatgc
     2641 tgacggcggc ctggctgcac ttcagcgacg cgatctgctt gccgcgacgt tcgaccacca
     2701 gcccctcctt ctcctggatg gcaccccggg ggccccactt gccgatgccg gtgtagacca
     2761 cgtacgcgtg atctccattg aagaatcgaa gccaggcgct gccgccggcg gcaaacacca
     2821 cggaggcgcc agcggctgcg gccgacggct cgatgcggcg tgaaggaatc tccagctcga
     2881 gcggaacgct gccgtcggcc ttgccgaaac ggtactggac atagcctcgc tgggcggtcg
     2941 acgccggatc ggcacagacc gacacccgcc tggcaccgat gcggcaggtg aacaccgtgg
     3001 tttccgcggc ggagcagaac gaggcggctc cggccggggc ggcctgaggt gccacaaccg
     3061 tcgcaggcgg ttcggcgggt ctcaacgaac tgaccagcgg caccgttcgc gactttctgc
     3121 aggatggatc gccctggcag tcctcgtgca tgacgcggcc cggcgtgtag gcgagcctga
     3181 cccgcttgcc ggtcagcttg ccggacgtca cgcacacctc gaaatcggcc cgctcggtga
     3241 attgccggcc ggcgtcgtcg cgcagcgtca ggtagcacgc ggcgtcgccc tcctgctgac
     3301 cgacgaggat gccttcggtc gccttgacct cgttgccgat cttcacggtg ggcagggtct
     3361 gcgaaccggc aggcccggcc agcaggcagc acagcagcaa ccagccgcga tgcggcatcg
     3421 aagcgaacaa catcacgaac tcctacagaa gggaccggcg aggatggagg cggaacgtta
     3481 tcgcgtcgaa gggcctgcag ccggggtcgc ggcagctcgc tcggcggctt tccggtagaa
     3541 gtccgcagcc tcgagacggc tgagctgcgc agcgaggaaa tcaggggtct cgcgcagctg
     3601 ctcgaccttc tcgaggccgc cgagccagcg gatcatccgg ccctcgcgga aatactggcg
     3661 atcctcgacg cgcgtgacct gccggccgga ctcggtgtag ccccggatgg cctcgtacac
     3721 gaagaccagt tgccgcccag caaagaagta ctcgccgacg acgtcgcccg aatcgtcgcg
     3781 gtcggtcact tccagcttgc gcacgccttc ggcgtcggac caggcgcgga cctcgctgac
     3841 gtattcaaca tcggggcgcc gggcccggaa cgagaccctc gtcatgtgcg gcaacttggc
     3901 gttcacctcg gcatagacct ggcgcgcctg cgacgtgtcg acctcggcgg cagcggccgg
     3961 cccccaggcc agaagcgacg ccagcgtcag ggcgttgacc cagcaggccg atcccatcag
     4021 ccgagcaacc ccgtctcgcc cggatggctc ggcagcacca ccggcgtctg cggcttccag
     4081 cccttcttgc ggtgatcggc caccacgttg gtgtagatgc cgccgcgcac gtaccagcgg
     4141 gcggtgatgc gggcatagcg aggctgggtg acggcaacga tcttcgtcag gatgtcgttc
     4201 gtcaccttct cgtggaacgc gccctcgtca cggaaactcc acatgtacat cttcaggctc
     4261 ttcagctcga cgcacaggtc gtcggccacc atgtcgatga cgaagtgcgc gtagtcgggc
     4321 tggccggtca gcggacacag gcaggtgaac tccgggatct ggaactggat cacgtagtcg
     4381 cgctcgggcg acgggttcgg gaacacatgc agttccttcg acggcgcggt cggcggattg
     4441 atcggcgcga cgcgctgctt cgcgggctcg ggcgggacgc tggatttctt cggggttcgg
     4501 gccatgggtc ggtctcgtcg agatgagtgc tcagggaatg ccgagggtct tgtgggtctg
     4561 cacgctgagt cgccactgcg gatgcaccag gcagtagtcg acagccagcc gcgtgttgcg
     4621 acgttgttcc agtccgtcca tcggctgcag gaagaacagc ccgaagtcca gtccttcgaa
     4681 atgctcgggc atcgccccgg gctgcggaaa caccagcttc agttcgtggc cgtgcgtcag
     4741 caccagttcg gcgccggctt tcgggctgac acagatccag tcgagcccag cgggcgcctg
     4801 ctgcgtgccg ttggtctcga cggcgacctc gaagccgagc gcatgcagcg cgtcgatcag
     4861 cggctcgtcc agttgcagca acggttcacc gcccgtgcac acgaccagcg gccggcccgg
     4921 tcgatcggcg ggccaccgct gcgcgatgtg cgcggcgagc gcctgcggcg tgtcgaactt
     4981 gccaccgccc ggaccatcgg tgccgacgaa ctcggtgtcg cagaaacggc ataccgcctc
     5041 ggcgcgatcc gcctcgcggc ccgaccacag gttgcagccg gcgaagcggc agaacaccgc
     5101 cgcacggccg ctctggccgc cctcgccctg cagcgtgtag tagacctctt tcaccgcgta
     5161 ggtcatgctg gctccattcc gctcacatcg gcccggacag ggtcgcgctg ccggtcgaca
     5221 cgatggcgcc gcagccggcg gtctcctcga ggtcgacgcg gtcgacctgc ggcagctgca
     5281 ggcgcgcccg ctccagcacc cagtgcgcga tgctcgcggt gtcgcagtcg gccaggtcgg
     5341 cgatctcgtg gatcggccgg tggtcgagca tctggaagat cggatcgaac gccgccttca
     5401 cgtcgccgaa atcgaacgcc cagcccagcc agtcgtcgag cggcgccgac aggtgcagcc
     5461 gcagcgtgta ggtgtggccg tgcaggcgac gcaggccgct gccctcaggc gcgtgcttca
     5521 gttgcagcgc gccgtcgagc gtcatctgct tccagatgcg gtggcgctgg ccatcgaaga
     5581 ccgcgccgca gctgccggtc tcgaacacgg tgatcgtcga cagcccgggc agctgcggcg
     5641 cgatgcgcgc ccacagccag ctcgccagca gctcgctggt cgggttctcg agaccaggca
     5701 gttcgttcag gcaggcgtag ttcaactgga aatggatcgg tgcccagatc gcgtccagat
     5761 cgtcgtggct gaggcccgat ccgctgtccg atgcatgcac gatcgcctcg aaaccgtggc
     5821 cgtgcatcgt cccgcatttg tgaccgagcg gcacgtgcgg cagccggtgc gccgaatgga
     5881 acgcaaagcg ccgccacagc cgagtgcgtc ccgtcgcgtc gaggtcggca ccgcactgcg
     5941 cggtgctctg aacgccgacg tcgacgcgcc ccggcagggc cagccggtcg cgcacccagg
     6001 cggccaggtt cgcgtcgctg ggctgcggca gatgctcgtt gagcagccgg tgatcgagca
     6061 cgccgacgca ggcctgcagc tcatcgcgca ggcgatcgac ctcgccgccc tggaagcggg
     6121 cccagccggc cggcaattcg cagcgcacct tcgcgacgaa cccgtggccg tgcaggcggc
     6181 ccgacggatg ccccacaggc accgcatcga gctgacgtgc tgcctggaac gccgccgacg
     6241 cgctgaacaa ggtccggacg gaggctgtca tcttcagacg atcccgaagc gcgccagcag
     6301 cggatcgcgc acgcccgcct cggcgaagcc cttcgcgcgc agcaggcacg agtcgcagcc
     6361 gccgcagggc cgaccatcca caccggggtc gtagcaactg ctggtctggc cgtaatcgac
     6421 gcccagccgc agcccctcgc ggatgatctg ctccttcgac aggtcgatca gcggcgcgtg
     6481 gatcttcagc cgctgtgtgc cttcgacgcc ggcctgcgtc gccaggttgg ccatcgcctc
     6541 gaaggcggcg atgtactcgg gccggcagtc cgggtagccg gagtagtcga gcgcgttgac
     6601 gccgatgaag atgtccgacg agcccagcgt ctcggcccag gccagcgcga agctcaggaa
     6661 gaccgtgttg cgcgccggca cgtaggtgat cgggatgcca tgggccatct cgccggcggt
     6721 gcggcctttc ggcacgtcga tgtcggcggt cagggccgac ccaccgaact ggcgcaggtc
     6781 gatttcggcc acgacgtgcc gggccacgcc ctggctgcgc gcgaccacat cggcggctgt
     6841 cagctcgtgc acgtgacgct gtccgtagcg gaagctgagt gcgcagggtt cgaagccgcg
     6901 ttcgcgcgcg atcgccagca cggtggccga gtccagcccg ccactcagca gcaccacggc
     6961 aggcggtcgc cccgaaacac tgtccgtcat ccccaagctc cagaacgatt ctcagccgaa
     7021 aaacccgcca gtgtagtggt cgccccatgc ccgcgagggc ctcaaaaccg gcgccgtccg
     7081 ccgagcccgg acaatcgccc gatgtctgac acaccgaacc tgcccaaccc acgacacgcg
     7141 tcccgcgtgc catcgcacat cggcaaggcg caattcgagc gcatccgcca ggcggcctac
     7201 cggcttccgc aggtcgtgaa gccgggcgac tacatcgccg agcgcagcgc cggcttcgag
     7261 gaacacctcg acaagctcgg cctcgtgctg ctgcagaaac cggtggccgg cgcccggccg
     7321 agccgctgga tcgcgcccga cctgatggac agcgcgctga cccacctcga cgcggtgggc
     7381 aacggcccgg cggtgcgccc gctgatgctg gaacgcaccg ccgcgctcga agccatgggc
     7441 gtgacacgcg acgccggcgt gtggacgcgt ctgatcagcc acgcgctgac gctgtcggaa
     7501 gccggctgga aggtgccggt gctgttccgc gtgccgttga acgacgcggc cgtgtgggac
     7561 ttctatcgca ccggcgacag cgccgcgctg caggctgcca tcggcccgcc gacgccctac
     7621 ccgaacgccg ccgaactggt gacgcacctc gacgatctgg ttgcgcgcag ccgtgcgttg
     7681 aaaccgaacc acctcgagtc gctgctgccc cggctgcagt ccgaatgcgc gacgccgcag
     7741 gcgctggccg atctgaaggc cgcgctgatg cgcgcgcagg accgctggga gcaccgcgcc
     7801 tcggaactcg acacgatcga gggcctgaac agcgagctgg cgttccaggg ctaccccgac
     7861 accttcgaga aggcgcgacg gctgcagcgc agcgtcacgc tgtatgtcgg cccgcccaac
     7921 agcggcaaga cgcacgccgc gttcgaacgg ctggcccagg cgcacgacgg ggcctacctg
     7981 gcgccgctgc ggctgctggc gcttgagggc cgcgaccggc tggtcggacg cggcgtgccc
     8041 tgttcgctgc tgaccggcga ggagaacgtg ccagccgacg gcgcgcgcgt ggtctccagc
     8101 acgatcgaga tggtcggcac caacaagccg atcgacgtcg cggtgatcga cgaggcgcag
     8161 atgatcttcg acgtctcgcg cggctgggcc tggacccagg ccatcgtcgc ggtgccggcc
     8221 aatgaagtca tcatcatctg ctcggcctat gcggtgccgg ccatcgagaa cctgctcggc
     8281 ctgtgcggcg agcgctgcac ggtgcggcat ttcgagcgca agcagcacgt cgagttgctg
     8341 ccgcacccga tcgccacttc ggcgctgcaa ctcggcgacg ccgtggtcgc gttcagccgc
     8401 cgcgacgtgc tgagcctgcg cgaccgcatc gctgccgacg gacacccggt gtcggtgatc
     8461 tacggcgcct tgccgcccga ggtgcgccgg cgtgaagccg aacgcttcgc cagcggcgag
     8521 tcgcacatcc tggtcgccac cgacgcgatc ggcatgggcc tgaacctgcc gatccggcgc
     8581 gtgctgttca gcacgatgac caagttcgat ggcgtcgccg accggccgct caacgaatcc
     8641 gaggtgcatc agatcgcggg ccgcgccggc cgcttcggca tccacgagga aggcttcgtc
     8701 ggcgtgctga aggacgccga gccacaggcc gcgaggatgc tcaaggagct gctgccgaag
     8761 acaccgcgcg ccccgcgcga cttcaaggcc ccggtggcac cgaacggctg gcacgtcgac
     8821 accatttcat cgcgactgca caagaccaag ctgcgcgagg tgctgggcgt gttcatggaa
     8881 cagctcaagc tcgacgacgc gcacttcgcg gtggccgagc tcgatcagat gctcgacctg
     8941 gccgagcagc tcgaccgctg cgcgccaaag ctcagcctga aagagcgctt catctacgcg
     9001 caggccccgg tcgacacccg caccgacagc caggtccagg agttcctcga ctggaccagc
     9061 agtcacgccc tcaccggcaa ggccggcagc ccgtggttcc tgaaggacgt cgacggccac
     9121 agccggcttg accgcatgga gcaggcgctg cgcgcctgca cgctgtggct gtggctcgac
     9181 ctgcgcttcc ccggcgtcta cggccacatc gatgcggtgc tcgaactgcg cagccagctc
     9241 aacgacggca tcgagcggca actcaagggc aagcggccgc tggcgcaggt gaggcagcgg
     9301 gcgcggcgct gaggcgcggg ccaacggcgc agcatgcgag gacgtggcgc cggggcggag
     9361 tcgatacgca gcaacgtttg cggcgacaaa gccccctccc cgccaggggc cgatacctga
     9421 tcgacagcac gctgcgcgga tcgccccccg tacattgata atccaatttt caaatgaaga
     9481 aagaccgccg cccatgaaaa ccgccctgct cttcgcagtc gtttcgaccg ctgccattac
     9541 cctgctgccg gtcgcgaccg cccaggccca ggtctcccag gccgaggccc agatcaatgc
     9601 gctggaaggg ctgttcggca agcacgccgg cgttcgtcgc tcgcaggcca agggcgtgtg
     9661 tgccagcggc tatttcgtcg gcaacgcggt ggggcgctcg ctgtcgagtg cggccgtctt
     9721 caacggcgac cgggtgccgg ccggccagcg ctgcgcctcg acctccaacc gca
//
LOCUS       DAOORV010000124         9760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8646_length_9760_cov_6.840804, whole genome shotgun sequence.
ACCESSION   DAOORV010000124 DAOORV010000000
VERSION     DAOORV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8646_length_9760_cov_6.840804"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..993)
                     /locus_tag="PKV56_14120"
     CDS             complement(181..993)
                     /locus_tag="PKV56_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOX68997.1"
                     /translation="MLGRACSAWRRWRDDRICRRHAIPEALWTLTLARYPFLTWRSTD
                     EQILLRRLTTLFLARKEFSGAQGFVVTDEIAVAVAAQAVLPVLKLGLGLYDGFVGIVM
                     HDDAVVAQREVVDDDGVVHHFEEELSGEAMHGGPVMLSWTDVAAASMSDPWGYNVTIH
                     EFAHVIDMVDGMADGVPAMRDSRSRQAWLSVMEPAYADFCQRVDADQPVAIDPYAAES
                     IDEFFAVASETFFVDPDTLHQAMPRVYDLLADYFGQSPRNDLQARARTPASG"
     gene            complement(996..1802)
                     /locus_tag="PKV56_14125"
     CDS             complement(996..1802)
                     /locus_tag="PKV56_14125"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOX68998.1"
                     /translation="MEAGGPAALPTFWEVHAAAEWRTIDFISDLHLAEDTPRTFDAFA
                     SYLSQTPADAVFILGDLFEVWVGDDSRHEGFEARCVDVLGDCASRCAMGFMVGNRDFL
                     VGAEMLKSCGVLALSDPTVLIAFGERLMLTHGDALCIADVPYQRFRSMVRSPAWRGDF
                     LSRPLSDRYKAARDVRQESERRKREHAGGQYVDIDVATAVRWMHEAGTPSMIHGHTHM
                     PGSETLAPGFQRHVLTDWDFDHCEGSPRAGVMRWKSDGIQRLTLAQAVTN"
     gene            complement(1810..2310)
                     /locus_tag="PKV56_14130"
     CDS             complement(1810..2310)
                     /locus_tag="PKV56_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX68999.1"
                     /translation="MSKKVELHTSHGLIVAELDDVKAPLSTANFIAYVEKGHFDGTIF
                     HRVIKGFMIQGGGFDAEMKQKACDDPIKNEASNGLKNQRYTLAMARTSDPHSATAQFF
                     INANDNEFLNFKAENASGWGYAVFGKVVAGTEIVDAIERVKTGRKGFHDDVPTEPVVI
                     TKAVLA"
     gene            complement(2351..2920)
                     /locus_tag="PKV56_14135"
     CDS             complement(2351..2920)
                     /locus_tag="PKV56_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX69000.1"
                     /translation="MRFTAAFGIATLCLSLAVASSNVLAQKVNLTTSLGEVVLELDAG
                     KAPKTVDNFVQYVKAGHYNGTVFHRVIENFMIQGGGFAADMKEKSVRAPIGLESRNGL
                     SNVRGTVAMARTMDPNSATAQFFINVKDNDFLNAANSRDGNGYAVFGKVVSGMDVVDR
                     IRAVKTGNRGPFQDVPLEPVLIIKATVQE"
     gene            complement(2964..3494)
                     /locus_tag="PKV56_14140"
     CDS             complement(2964..3494)
                     /locus_tag="PKV56_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX69001.1"
                     /translation="MPCLRPFRVFVATLVLLSCGSVRANDVTDIADLFHTGKQAEALA
                     RIDTLLGARAERPDLRFLKGVLLVESRRVDEAVSVFQQLTEEFPELPEPYNNLAALYA
                     ARGDFEKARAALDGALRANPGFAVAHENLGDVYAQLARLSYQRALKLDPSNTTIPPRL
                     ALLRELSDPSARKPSR"
     gene            3751..5133
                     /gene="cysS"
                     /locus_tag="PKV56_14145"
     CDS             3751..5133
                     /gene="cysS"
                     /locus_tag="PKV56_14145"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOX69002.1"
                     /translation="MTLRIFNSLSRETEVFTPIDANHVRMYVCGMTIYDLCHVGHARF
                     MMAFDVTRRWLEVLGYRVTYVRNITDIDDKIIKRAVERGIPIRQLTEEMTQAMHEDVA
                     ALGILPPTHEPRATEHVPEMLSMIGTLESKGLAYRSPNGDVNYAVRKFPAYGRLSGKS
                     LDQLRAGERVAVLDGKDDPLDFVLWKSAKDSEPEDAKWDSPFGRGRPGWHIECSAMSC
                     ALLGVHFDIHGGGLDLQFPHHENEIAQSEGASGQRFVNVWMHNGFLNVDNEKMSKSLG
                     NFFTIRDVLKRYDGETVRFFMLRTHYRSPFNFSDTNLDDARQSLRRIYTALASVDLPT
                     DDDHAIDWSQPQAATFRAEMNEDFNTPGAVAVLFDLAGEVNRTRSPQAARLLKELGAT
                     LGLLQLPAQSYLQAGSGLDESRIAELIAERNAAKVARDFARADQIRQELAAQSILLQD
                     SPQGTTWVKA"
     gene            5133..5780
                     /locus_tag="PKV56_14150"
     CDS             5133..5780
                     /locus_tag="PKV56_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOX69003.1"
                     /translation="MLAGSAAPPAFWDDACRHLAKRDRVMRKLIPKCGDSHLKCRGDA
                     FTTLARSIIGQQISVKAAQSVWERFIAAVPGGVLHLQPDSVLRLAAPEMRAVGLSARK
                     VEYINDLALHFSAGSVHENQWEQMEDQAIIDELVAIRGIGRWTAEMFLIFHLMRPDVL
                     PLDDAGLIKGISVNYFSGEPVSRAEAREVGEAWSPFRSVATWYIWRSLEPLPVDY"
     gene            5830..6801
                     /locus_tag="PKV56_14155"
     CDS             5830..6801
                     /locus_tag="PKV56_14155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOX69004.1"
                     /translation="MSKRHFLEFEQPVAELETKIDELRYVQNESAVDISEEIDRLSKK
                     SLQLTKDIYSSLTPWQVTQIARHPQRPYTPDYVSEIFTDFHELHGDRHFADDQSIVGG
                     LARFNGQACMVIGHQKGRDTRERGLRNFGMSRPEGYRKALRLMKLAEKFSLPIFSFVD
                     TPGAYPGIGAEERGQSEAIGRNIFEMAQLEVPIIVTIIGEGGSGGALALSVGDQLLML
                     QYSIYSVISPEGCASILWKTSERASDAAEQLGITAHRLKALGLVDKIVNEPVGGGHRD
                     IKQMAAHLKRALNDALRQVSDLKPKELLQRRYERLQTYGRFTDTTDR"
     gene            6842..8146
                     /gene="tilS"
                     /locus_tag="PKV56_14160"
     CDS             6842..8146
                     /gene="tilS"
                     /locus_tag="PKV56_14160"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOX69005.1"
                     /translation="MTSRRVAVAYSGGRDSTALLHAVLMSVRDTGTSVAALHVHHGLS
                     GQADAWERHCRDQCARWQAEGFPVSFAACRLRTRPQPGDSIEAWARRERYAALGRLAR
                     THGADVVLLAHHRRDQAETLILQALRSAGVAGLAGMPRAIERDGIHWLRPWLDVPRST
                     IEAYVESLGLSYIDDDSNTDERHARNRLRLRVWPALVQAFPEAEPALAAAATWAQQAQ
                     ACLDELASDDLALLHGPAGLQLVGLGQLSAARQVNALRAWLRLQTGAAPPASLLARLS
                     DELFGVGAASWPLAGGLLRRHRGQLTWSRPVAARSPSKTALEAHLHIRRAGIHRLPGW
                     GGNLRVVRVKEGGVPLAWLAQAELRARSGGEQFQAGLGRPARSLKKQFQAAELPAWER
                     SGPLVYSGGQLVFVPGLGIDARVIGLPGQPLVTLLWEPADRM"
     gene            8242..9510
                     /locus_tag="PKV56_14165"
     CDS             8242..9510
                     /locus_tag="PKV56_14165"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOX69006.1"
                     /translation="MALTVHKYGGTSMGSPERIRNVAKRVAKWARAGHQMIVVPSAMS
                     GETNRLLGLAKELSPASSSPELMRELDQIAATGEQVSVGLLAIALQAEGMQAVSYAGW
                     QVPIRTDSAFTKARIQSIDDKRVRADLAAGKVVVITGFQGVDDQGNITTLGRGGSDTS
                     AVAVAAALKADECLIYTDVDGVYTTDPRIVPEARRLNSISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPMRVLSSFTPWDIDIVEEAKSGTLITFEEDENMEQAVVSGIAFNRDEA
                     KISLIGVPDKPGIAYQILGPVAEANIDVDVIIQNVSHEGKTDFSFTVHRNDYARTMDL
                     LKATVVPKTGTATLSGDTKICKVSIVGIGMRSHVGVASKMFSTLSAEGINIQMITTSE
                     IKTSVVLDEKYMELAVRALHKAFDLEQAPA"
     gene            9556..9648
                     /locus_tag="PKV56_14170"
     tRNA            9556..9648
                     /locus_tag="PKV56_14170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9590..9592,aa:Ser,seq:gct)
BASE COUNT         1762 a         3137 c         3159 g         1702 t
ORIGIN      
        1 cggcggtcgc ctgacggtcg atgtggatgc cgacggcaag gccgttctgg acatccagcc
       61 gcccaagaag agcgacaagc ccaaggccga gcccgcaacg gcctgacctc gaaagtcagc
      121 tgttctcgcg catcggggcc cgtcggaaga cgggccccgc ctttttgtgc acggccggat
      181 tcagccagac gccggagtcc gggctcgagc ttgcagatcg ttgcgagggg actggccaaa
      241 gtagtcggcc agcaggtcgt acacccgagg catggcctgg tgcagggtgt cgggatcgac
      301 gaagaaggtc tctgaagcca ctgcgaagaa ctcgtcgatt gattcggcag cgtaggggtc
      361 gattgcgacg ggctggtctg catccactcg ctggcagaaa tcggcgtagg cgggctccat
      421 cacggacagc cacgcctgcc gagaccggct gtcgcgcatg gcaggcaccc cgtcggccat
      481 gccatccacc atgtcgatga catgcgcgaa ttcgtggatg gtgacgttgt agccccaggg
      541 atcggacatg ctcgccgcag cgacatcggt ccaggacagc atgaccggcc cgccatgcat
      601 cgcctcgccc gagagctctt cctcgaaatg atggacgaca ccgtcgtcgt cgacgacctc
      661 gcgctgggcg accaccgcat cgtcgtgcat cacgatgccg acgaatccgt cgtacaggcc
      721 cagtccgagc ttcaggaccg gcagcacagc ctgtgccgca accgccacgg caatttcatc
      781 cgtgacgacg aatccctgcg ccccgctgaa ttccttgcgc gccaggaaca gggtggtcag
      841 cctgcgcagc aggatctgtt cgtcagttga gcgccacgtc aggaaggggt acctcgcgag
      901 cgtcagtgtc cacaaggctt ccgggatcgc atggcggcgg cagatcctgt cgtcccgcca
      961 gcgtcgccaa gcgctgcacg cccgaccaag cacggtcagt tcgtgaccgc ctgcgccagc
     1021 gtcaggcgct ggatcccgtc ggacttccag cgcatcacgc ctgcgcgagg gctgccttcg
     1081 cagtggtcga agtcccagtc ggtcaggaca tgacgctgga atcccggcgc caaggtctcg
     1141 ctgcccggca tgtgggtgtg accatggatc atcgatggcg ttcctgcctc gtgcatccag
     1201 cgcacggcag tggccacgtc gatgtcgacg tactggccac ccgcgtgttc acgcttccgg
     1261 cgttcgcttt cctgccggac gtcacgcgcc gccttgtacc gatccgacag cgggcgggac
     1321 aggaaatcac cgcgccaggc cgggctgcgc accatgctgc gaaaacgctg gtaaggcaca
     1381 tctgcaatgc acagtgcatc accgtgagtc agcatcaagc gctcaccgaa ggcaatcagg
     1441 accgtcgggt cggacaaggc cagcacgcca caactcttca gcatctcggc tccgaccagg
     1501 aagtctcgat tgccgaccat gaaccccatc gcacagcgcg aggcgcaatc gccgagcacg
     1561 tcgacacacc gcgcctcgaa tccctcgtgg cgggagtcgt cgccgaccca cacctcgaac
     1621 agatcaccga gtatgaagac cgcgtcggct ggtgtctggc tcaggtacga cgcaaatgca
     1681 tcgaaggtcc gcggggtgtc ctcggcaagg tgcaggtcgc tgatgaagtc gatcgttcgc
     1741 cactctgcag cggcgtgcac ctcccagaac gttggcaacg cggccggccc tcccgcttcc
     1801 atggtcctgt caggccagaa cggccttggt gatcacgacg ggctcggtcg gcacatcatc
     1861 gtggaacccc ttgcgaccgg tcttgacccg ttcgatggcg tcgacgattt ctgtcccggc
     1921 gaccaccttg ccgaagaccg catagcccca gccgctcgcg ttctcggcct tgaagttcag
     1981 gaattcgttg tcgttggcgt tgatgaagaa ctgggcggtg gccgaatgcg ggtcggacgt
     2041 gcgagccatg gccagcgtgt agcgctggtt cttcaggccg ttggatgcct cgttcttgat
     2101 cggatcgtcg caggccttct gcttcatttc ggcgtcgaat ccaccgccct ggatcatgaa
     2161 gcccttgatg acgcgatgga agatcgtgcc gtcgaagtga cccttctcga cataggcgat
     2221 gaagttggcg gtgctcaggg gcgccttgac atcatcgagt tcggcaacga tcagtccgtg
     2281 cgaggtgtgc agttctactt tcttgctcat ggtgcggttc ggtgagttga gatggtgtgt
     2341 tgagtgcggg tcattcctga accgttgcct tgatgatcag aaccggctcc agcggcacgt
     2401 cctggaaggg accgcggttt ccggtcttga cggcccggat gcgatcgacg acatccatgc
     2461 ccgagacgac cttgccgaag acggcgtagc cgttgccgtc gcgggaattg gccgcattga
     2521 ggaaatcgtt gtccttcacg ttgatgaaga actgggccgt cgcggagttc gggtccatcg
     2581 tccgggccat cgccaccgtg ccgcgcacgt tgctcagccc gttgcggctc tcgagcccga
     2641 tcggcgctcg caccgacttt tccttcatgt cggctgcgaa tccgccgccc tggatcatga
     2701 agttctcgat gacccggtga aaaaccgtgc cgttgtagtg ccccgccttg acgtactgaa
     2761 cgaagttgtc cacggtcttc ggcgccttgc ccgcatcgag ttcgaggacg acctcgccca
     2821 ggctggtggt caggttgacc ttctgggcca gaacattcga ggaggcgact gccagcgaca
     2881 ggcagagagt ggcgatgcca aaggcagcgg tgaaacgcat gaggaaaact ccgtggggtt
     2941 cgagccggcg gcgacagccg gaatcagcga gaaggcttgc gggcggatgg gtcggacagt
     3001 tcgcgcagca gtgccaggcg cggggggatc gtcgtgttgc tcgggtcgag cttgagcgcg
     3061 cgctggtaag aaagccgggc caactgcgca tacacgtcgc cgaggttctc gtgcgcgacc
     3121 gcaaaacccg ggttggctcg caacgcaccg tcgagcgccg cacgggcctt ttcgaaatcg
     3181 ccccgggctg cgtacagcgc cgccaggttg ttgtagggct cgggcaactc ggggaactct
     3241 tccgtcaact gctgaaacac cgaaacggcc tcgtcgacgc gcctggactc gaccaggagc
     3301 acccctttca ggaatctcag gtcggggcgc tccgcgcggg cgccgagcaa ggtgtcgatc
     3361 ctggcgagtg cttcggcctg cttgccggtg tggaacaggt ccgcgatgtc agtcacatcg
     3421 ttcgcgcgga cgcttccaca agacagcagc acgagcgtgg caacgaagac gcggaatggt
     3481 ctgaggcagg gcatggtcag ggacgtgatg aacgggagca gtcgaggcgc ctggaggcaa
     3541 cccggtcagg gcggttcatg aaagggtcgc cggtatactg gttttgcagc ggattgtagg
     3601 tgtgcggttc atgagtgaaa tccccgggta tcggatgttg ctacgggaga ttcgaccggt
     3661 cagcatctcg cccttcagct tccagaattt ccagtccctt tccggcgtgg ccccgagtca
     3721 agccgtgagc acaccgatcc gacccggccc atgacgttgc gcatcttcaa ttcgctgtcc
     3781 cgcgagacag aagtcttcac ccctatcgat gccaaccatg tccgcatgta cgtctgcggc
     3841 atgaccatct atgacctgtg ccatgtgggt catgcgcgtt tcatgatggc cttcgatgtg
     3901 acccgtcgct ggctcgaggt gctgggctat cgggtgacct acgtgcgcaa catcaccgac
     3961 atcgatgaca agatcatcaa gcgggcggtg gagcgtggca tcccgatccg gcaactgacc
     4021 gaagagatga cccaggcgat gcacgaggat gtggccgcac tcggcatcct tccgcccacg
     4081 catgagccga gggcgaccga gcatgtgccc gaaatgctgt cgatgatcgg cacgctcgaa
     4141 tccaagggcc tcgcctaccg ttcgcccaat ggcgatgtga actacgccgt ccggaagttc
     4201 cccgcttatg gtcgactgtc gggcaagtcg ctcgaccagt tgcgggcggg cgagcgcgtg
     4261 gccgtgctcg acggcaagga cgacccgctg gatttcgtgc tctggaagtc ggccaaggac
     4321 agcgaacccg aagacgccaa gtgggacagt ccgttcggtc gaggtcgccc gggttggcac
     4381 atcgaatgtt ccgcgatgag ctgtgccctg ctcggtgtgc atttcgacat ccacggtggc
     4441 gggctcgacc tgcagttccc gcaccacgag aacgagatcg cccagagcga aggggcgtcg
     4501 ggccagcgct tcgtcaatgt ctggatgcac aacggatttc tcaacgtcga caacgagaag
     4561 atgtcgaagt cgctcggcaa cttcttcacc atccgcgatg tgctcaagcg ctatgacgga
     4621 gagactgtcc gcttcttcat gctgcgcacg cattaccgca gcccgttcaa tttcagcgac
     4681 acgaacctcg atgacgccag acagtcgctg cggcgcatct acaccgcact ggcgagcgtc
     4741 gacctgccaa cggatgacga ccatgcaatc gactggtcac agccgcaggc cgcgaccttc
     4801 cgcgcagaga tgaacgagga cttcaacacg ccgggcgctg ttgccgtgct gttcgacctg
     4861 gcaggcgagg tcaatcgcac gcgatcaccg caggccgcgc gactgctcaa ggagttgggc
     4921 gcgacgctgg gcctcctgca actgccggcc cagtcttatc tgcaggccgg ctccgggctg
     4981 gacgaatcgc gcatcgccga actgatcgcc gagcgcaatg cggccaaggt cgcgcgtgac
     5041 ttcgcgcgtg ccgaccagat tcgtcaggaa ctggcggcgc aatcgatcct gttgcaggac
     5101 tctccccagg gcaccacctg ggtcaaggca tgatgcttgc cgggtcggcc gcgccgccgg
     5161 ccttctggga cgacgcgtgc aggcacctcg cgaagcgcga ccgggtgatg cgcaagctga
     5221 ttccgaagtg cggcgacagc cacctcaagt gtcgcggaga tgccttcacg acgctggccc
     5281 ggtcgatcat cgggcagcag atctcggtca aggcggcgca gtctgtctgg gagcgcttca
     5341 tcgcggcggt gcccggcggc gtgctgcacc ttcagccgga cagcgtgctt cgccttgccg
     5401 cacccgagat gcgcgcggtc ggcctgtcgg cgcgcaaggt cgaatacatc aacgacctgg
     5461 ccctccattt ctctgccggc tccgtgcacg agaaccagtg ggagcagatg gaggatcagg
     5521 ccatcatcga cgaactggtg gcgatccgcg gcatcggtcg ctggacggcc gagatgttcc
     5581 tcatctttca cctgatgcgc cccgacgtgc tgccgctcga cgatgccggg ctcatcaagg
     5641 gcatcagcgt caactacttc agcggcgagc cggtatctcg cgccgaggcg cgcgaggtgg
     5701 gcgaggcctg gtcgccgttc cggtcggtcg ccacctggta catctggcgc agtcttgaac
     5761 cgctgccggt agactactga atggtttttt ggcggctgat gtcgcctgtt cctgcgagaa
     5821 gaccaacgaa tgagtaaaag acactttctc gaattcgaac agcccgtggc tgaactcgaa
     5881 acgaagatcg acgaactgcg ctatgtgcaa aacgagtcgg ccgtcgacat ctccgaagag
     5941 atcgaccgcc tcagcaagaa gagtctccag ctcaccaagg acatctactc cagcctgacg
     6001 ccgtggcagg tcacgcagat cgcgcggcac ccgcagcggc cctacacgcc ggactacgtc
     6061 agcgaaatct ttaccgactt ccatgaactc catggcgacc ggcacttcgc cgacgaccag
     6121 tcgatcgtcg gcggcctggc gcgcttcaac ggccaggcct gcatggtgat cggtcaccag
     6181 aagggccgcg acacccgcga gcgcggcctg cgcaacttcg gcatgtcgcg acccgagggc
     6241 taccgcaagg ccctgcgcct gatgaagctc gctgaaaaat tcagcctgcc gatcttcagc
     6301 ttcgtcgaca cgcccggcgc ctatccgggc atcggcgccg aagagcgcgg ccagtcggag
     6361 gccatcggtc gcaacatctt cgagatggcg caactcgagg tgccgatcat cgtcacgatc
     6421 atcggcgagg gcggctcggg tggtgccctc gcgctcagcg tcggcgacca gttgctgatg
     6481 ctccagtact cgatctattc ggtgatctcg ccagaggggt gtgcctccat tctctggaag
     6541 acctccgagc gcgcctcgga cgctgccgag caactcggca tcaccgcgca tcggctgaag
     6601 gcactcggcc tggtcgacaa gatcgtcaac gagccggtcg gcgggggtca ccgcgacatc
     6661 aagcagatgg ccgcgcatct gaagcgagcg ctcaacgacg cgttgcggca ggtcagtgac
     6721 ctgaagccga aggaactgct ccagcggcgg tacgagcggt tgcagaccta cggtcgattc
     6781 accgacacga ccgatcgctg acactcggat ggggcggtgc atgaagccgc ctgcttccgc
     6841 aatgacctcc cgtcgtgtcg cggtcgccta cagcggcggc cgggattcca ccgcgctgtt
     6901 gcatgccgtg ctgatgtcgg tgcgcgatac cggcacgtcg gtggccgcgc tgcatgtcca
     6961 ccacgggctc agcggccagg ccgatgcatg ggagcggcac tgccgcgacc agtgcgcgcg
     7021 ctggcaggcc gaaggtttcc cggtgtcgtt tgccgcttgc cggctgcgca cgcggccgca
     7081 acctggcgac agcattgaag cgtgggcgcg ccgagagcgc tatgccgccc tcgggcgact
     7141 ggcgcgcact cacggggccg acgtggtcct gcttgcccat caccgtcgtg accaggccga
     7201 gacgctgatc ctgcaggcgc tgcgctcggc cggcgtcgcc ggtctcgccg gcatgccgcg
     7261 cgcgatcgag cgtgacggca tccattggct gcgtccctgg ctcgacgtcc cgcgcagcac
     7321 gatcgaggcg tacgtggagt cgctgggcct gtcctacatc gatgacgaca gcaacaccga
     7381 cgagcgccat gcgcgaaacc gcttgcgtct cagggtctgg ccggcactcg tccaggcgtt
     7441 cccggaggcc gaacccgcgc tggccgcagc ggccacctgg gcgcagcagg cgcaggcctg
     7501 cctcgatgag ctggcttcag atgacctggc gttgctgcac gggccggcag ggctccagct
     7561 cgtcgggctg gggcagctgt cggcggctcg ccaggtcaat gcgctgcgcg cctggctgcg
     7621 cctgcaaacg ggtgctgcgc cgcctgcatc gctgctcgcg cggctgagcg acgagctatt
     7681 cggggtgggc gctgcttcct ggccgctggc cggtggtctg ctgcgcaggc accggggaca
     7741 actgacctgg tcacgccccg tggcagcccg gtccccgtcc aagacggcat tggaggctca
     7801 cctgcacatc cgccgtgccg ggattcatcg tctccccggc tgggggggca acctgcgcgt
     7861 cgttcgagtg aaggagggcg gggtgccgct ggcctggctg gcgcaggccg agcttcgcgc
     7921 gcgatcgggc ggcgagcagt ttcaggccgg tctggggcgc cctgcgcgca gcctgaagaa
     7981 acagttccag gccgcggagc tgccggcctg ggagcgcagc ggacccctgg tctacagcgg
     8041 cggccaactg gtgttcgtgc cggggctcgg catcgacgcc cgcgtgatcg ggctgcccgg
     8101 acagcccctg gtgacgctgc tgtgggagcc tgcggatcgg atgtgagcac atgccgtccg
     8161 ccccggcgac ggcgcttgta aaatggaagg cttttccgat taccgcggca cagcccgtgc
     8221 cgcgtcaacg caccgattgc catggctctg acagtacaca aatacggcgg tacctcgatg
     8281 gggtcgccgg agcgcatccg caatgtggcc aagcgcgtgg ccaagtgggc tcgtgccggt
     8341 caccagatga tcgtcgtgcc ctcggccatg agcggcgaga ccaatcgcct gctcggcctg
     8401 gccaaggagc tgtctccggc gagcagttcc cccgagttga tgcgcgagct cgaccagatc
     8461 gctgccaccg gcgagcaggt ttccgtgggc ctgctggcca tcgccttgca ggccgaaggc
     8521 atgcaggccg tcagctacgc cggctggcag gtgccgatcc ggaccgactc ggccttcacc
     8581 aaggcccgca tccagagcat cgacgacaag cgggtgcgcg ccgacctggc cgcgggcaag
     8641 gtggtcgtga tcaccggttt ccagggcgtt gacgatcagg gcaacatcac cacgctcggt
     8701 cgcggcggat cggacacctc ggcggtggcc gtcgcagccg cgctgaaggc cgatgagtgc
     8761 ctgatctaca ccgacgtcga cggcgtctac acgaccgatc cgcgcatcgt tcccgaagct
     8821 cggcgcctca acagcatcag cttcgaggag atgctggaga tggccagcct gggctccaag
     8881 gtgctgcaga tccgctcggt cgaattcgcc ggcaagtacc gcgtgccgat gcgcgttctg
     8941 tcgagcttca cgccctggga catcgacatc gtcgaagagg cgaagtcggg caccctgatc
     9001 actttcgaag aggatgaaaa catggaacaa gccgtcgtat ccggcatcgc cttcaatcgc
     9061 gacgaggcca agatcagcct gatcggcgtg ccagacaagc cgggcatcgc ctatcagatc
     9121 ctcggcccgg tggccgaagc gaacatcgat gtcgacgtga tcatccagaa cgtgtcgcac
     9181 gagggcaaga ccgatttctc gttcaccgtg caccgcaacg actacgcccg cacgatggac
     9241 ctgctcaagg cgaccgtggt gccgaagacc ggcacggcca cgctgtcggg ggacacgaag
     9301 atctgcaagg tcagcatcgt cggcatcggc atgcgctcgc acgtgggggt tgccagcaag
     9361 atgttcagca cgctcagcgc cgagggcatc aacatccaga tgatcaccac cagcgagatc
     9421 aagaccagcg tcgtgctcga cgagaagtac atggagctcg ccgttcgtgc cctgcacaag
     9481 gctttcgatc tggaacaggc tccggcgtga tcgcgaagca catgcgagca gctagaatgc
     9541 gaggctgaat tctgcggagc tgtgaccgag tggccgaagg tgctcccctg ctaagggagt
     9601 atgtgggcaa aacctgcatc gagggttcga atccctccgg ctccgccaga ccataaaaac
     9661 aaaatcaaaa actcagacgg gttcggccgg tttatccgcc gccgaacgcg tagcgcagac
     9721 aaagcaaggg cccgatcaca agtcgggccc ttcgtttttt
//
LOCUS       DAOORV010000125         9717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8695_length_9717_cov_12.842269, whole genome shotgun sequence.
ACCESSION   DAOORV010000125 DAOORV010000000
VERSION     DAOORV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8695_length_9717_cov_12.842269"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKV56_14175"
     CDS             <1..512
                     /locus_tag="PKV56_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX69007.1"
                     /translation="RLPLTLAIIDGFLVGVGTSKFIFPLDSVVEVIENRLTETALDAG
                     GRSVVELRGQVLPVVSLRTLYALDSAEPERSSVVVIQSDGRRYGVMVDGLLGQHQTVI
                     KPLGRMFRSLRGMSGSSILGNGEVALIFDVGSLCRLAEQPPPGQSRTPPHHVKPAPYN
                     ANQTQGQAS"
     gene            509..2683
                     /locus_tag="PKV56_14180"
     CDS             509..2683
                     /locus_tag="PKV56_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX69008.1"
                     /translation="MNTPTPSWMARLVAGAELQQAAEQLKAHELARADAEAALAQVKA
                     RLDQVEAELKVRTDIMNVTSIVSEADKKGDILCVNEKFLEVSKYSQHELIGHGHNTTR
                     HPDMPKEVFKQMWATIGRGDIFRGVVKNRAKDGTPYYVDAVIAPILGENGKPMKYLGV
                     RYDITEAEIERQNARGVIAAIDASYAFIEFNLDGTVIKANEHFLSALGYRLDEIQGKH
                     HRMFVEPALASSGTYSQFWRDLNDGKAQKDVFKRISKTGQEVWIQAVYAPVKDEMGRV
                     FKVIKIATDVTAEVNANNMLRQAVEQAQQVTAAAKGGDLAQRIPLEGKSGPIGELCAG
                     VNSLMETTSVIFDDVGRVFGALAEGDLSQRITRDYDGIFAQVKNDANATSEKLASIIE
                     DVGRVFSGLANGDLSQRITRQAEGTFDQVKQDANASCDKLASIIEEVRAAADALTGAA
                     NQVSATAQSLSQSASEQASSVEQTTASIDLMSASIAQNSDNARVTDGMATKASKEAGD
                     GGQAVTQTVTAMKQIAQKISIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVR
                     KLAERSQEAAKEIGDLAGNSVSTAERAGKLLDEIVPSIQKTSELVQEIAAASQEQSQS
                     VTEIGGAMGQLSKATQQNASASEELAATSEELSGQAEQLQQSVAFFNLGGDATPQRRG
                     KADSGGTSERRAPNSPLRVAKAGGASASRPAATGTHSNFRPY"
     gene            2694..3218
                     /locus_tag="PKV56_14185"
     CDS             2694..3218
                     /locus_tag="PKV56_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX69009.1"
                     /translation="MSTHAPQRGGPSADEAPSQYLTFMLGDEVFAMDIRTVREIIQYG
                     PMTTVPLMPDFVRGVINLRGAVVPVIDLQARFGRPAAGISKKSCIVIFDAVRQGERVE
                     LGLLVDAVSEVIEISGDQIEPPPNFGASVRRDFIRGMGKVANRFVIILEPDKAFDVSE
                     MAQLCEAAQEPLAA"
     gene            3293..4105
                     /locus_tag="PKV56_14190"
     CDS             3293..4105
                     /locus_tag="PKV56_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOX69010.1"
                     /translation="MQALRDDTFRRITDLMYSAIGLSFTDNKKPLISSRLAPRIQRLG
                     LGGYEDYLALISSDEDAGEFQMAVDLLTTNETYFFREPAHYDLLEHELTRSKPQQLAV
                     WSAASSFGDEAYSTAMLLSDLQQLGRIGSQWKILGTDISDRVLRSAIEAVYPEDRLRN
                     VTPERLRRHCLRGDGASEGLIQISPKLRKHVSFGQLNLCQPIEDIGPFDVIFLRNVLI
                     YFDVPTKRDVVDRVLTQLRPGGLFFIGTAEGRVACNTPLQALAPGAFRKPSA"
     gene            4126..4611
                     /locus_tag="PKV56_14195"
     CDS             4126..4611
                     /locus_tag="PKV56_14195"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOX69011.1"
                     /translation="MKPMILEPLGQLHTLHPGDVVCAERGERMETLLGSCVAIVLTDP
                     RRTLGAMCHIVHASRATAGVAETGAYAEVALDTMYALLRERGIDPRQCEAYVYGGGNM
                     FPQLFNQTAHVGERNSRWALDALAEDGVRVLFHDLGGDTYRRLSWTVGPNAPQVTAVP
                     V"
     gene            4625..5683
                     /locus_tag="PKV56_14200"
     CDS             4625..5683
                     /locus_tag="PKV56_14200"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOX69012.1"
                     /translation="MAIRVYVVDDSAVVRQTLMHLLKGDAEIELMGSAPNPLIAGPVI
                     RKDRPDVLLLDIEMPGMDGLTFLRQIMEESPIPTVICSTLTTDGSRIALEALAAGAVA
                     VVAKPRLGLKQFLEDSRRELLQTLKTAARSRPRAGMSAPRSTSATTTAPKPPIAGLHA
                     LSVNKPVVIGSSTGGTQALEMVLTALPGDSPGIAIVQHMPEKFTAMYAQRLDGICAMN
                     IREAKDGDRLERGVVLIAPGGRHMQLRKAGGQYFAVVADGPPVNRHKPSVDVLFRSAA
                     DCAGKDLLALILTGMGDDGARGMKLLHDRGARTIAQNEETCVVFGMPKEAIKLGGVDE
                     ILPLQQMAAAIRSFDARG"
     gene            6088..8010
                     /locus_tag="PKV56_14205"
     CDS             6088..8010
                     /locus_tag="PKV56_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HOX69013.1"
                     /translation="MDVISHFAARFERTREEELSIEDYLAECKRNRVAYATAAERMLQ
                     AIGEPQTVDTRNDPRLSRLFANKVIKIYPAFAEFYGMEDSIEQVVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSIAERLKQLMQEVPFYAIKGSPVNESPLGLFDAAEDGPILEKE
                     YGIPRRYLNRILSPWAVKRLEEFGGDIRKFKVVKRYPSVLKQVGIAKTEPGDENNQDI
                     SSLVGKVDIRKLETFAQDDPDAYSFSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNYKGTEGFGAIPFDGVVLAHSNESEWTAFRNNKNNEAFLDRIYIVKVPYCLRVSEE
                     VKIYEKLLRGSSLANATCAPGTLKMMSQFAVLTRLKEPENSSLYSKMLVYDGENLKDT
                     DPRAKSFQEYRDYAGVDEGMSGISTRFAFKIISKVFNFDSTEVAANPVHLMYVLEQQI
                     EREQFPPETEQKYLSFIKEHLATKYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADY
                     WIQDQEYRDTDTGEVFDRASLNGELEKIEKPAGIANPKDFRNEIVNFVLRARANNQGN
                     NPVWTSYEKLRSVIEKKMFSNTEELLPVISFNAKASADEAKKHEDFVTRMVAKGYTPK
                     QVRLLCEWYLRVRKSS"
     gene            8038..9318
                     /locus_tag="PKV56_14210"
     CDS             8038..9318
                     /locus_tag="PKV56_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HOX69014.1"
                     /translation="MFQQIIDRRLAGKNKSIGNRERFLRRHKEQIREAVKRAVDGRGI
                     RDLAQGEDIHIPKRDISEPVFGHGQGGKREMVHPGNQEYVRGDRIERPKGGGGSGSGG
                     GQASDSGEGEDDFVFHLSKEEFMQVFFDDLALPNLARTTLSETPEFKTHRAGYSSDGN
                     PSNLHVVRSMRGAIGRRIAIGSESRRELHRLEAHLEHLLRHPEPNPHAHEIPPLRLEI
                     EALRTRLGRIPYLDPIDLRYRSRVRVPMPTSKAVMFCLMDVSGSMDEARKDLSKRFFI
                     LLYLFLTRHYEKIDIVFIRHHTQAQEVDEQNFFHSTETGGTVVSSALVLMEEIARARY
                     PTAEWNIYGAQASDGDNWHHDSGRCRDILANKILPLCRYFAYVQVAEEEQNLWDEYAA
                     LAAESKAFAVRKATDASQIYPVFRDLFRKEGVPA"
     gene            9315..>9717
                     /locus_tag="PKV56_14215"
     CDS             9315..>9717
                     /locus_tag="PKV56_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOX69015.1"
                     /translation="MSTAITPPWGEDGRHEQHSGGLRRRAADLIAPPRSKEPLPSPND
                     WSFELIDRYHEVIRETAESFGLDTYPNQLEIITAEQMMDAYASVGMPVNYRHWSYGKE
                     FISTEKNYRRGHMGLAYEIVINSNPCISYLME"
BASE COUNT         1942 a         3228 c         3100 g         1447 t
ORIGIN      
        1 tccgcctgcc gctgacgctg gccatcatcg acggcttcct cgtcggcgtg ggcacttcca
       61 agttcatctt cccgcttgac tcggtggtcg aggtcatcga gaaccggctg accgagaccg
      121 cgctcgacgc cggcggccgc agcgtcgtcg agctgcgcgg ccaggtgctg cccgtggtga
      181 gcctgcgcac gctgtatgcg ctggattccg cggagcccga gcgcagcagc gtggtcgtca
      241 tccagtcgga cggtcgtcgc tacggcgtga tggtcgacgg actgctcggc cagcaccaga
      301 cagtcatcaa gcccctcggc cgaatgttcc gcagcctgcg cggcatgtcc ggctcctcga
      361 ttctcggcaa cggcgaagtc gcattgatct tcgatgtcgg ctcgctgtgc cgtctggccg
      421 aacagccacc tcccgggcag agcagaacac cgccccacca cgtcaaaccc gccccataca
      481 acgccaatca aacacaagga caagcatcat gaacacaccg accccctcat ggatggcgcg
      541 ccttgtggcc ggcgccgaac tccagcaagc cgccgagcaa ctgaaggccc acgagctcgc
      601 gcgcgctgac gccgaggccg cgctggccca ggtgaaggcc cggctggatc aggtcgaagc
      661 cgagctgaag gtgcgcaccg acatcatgaa tgtcaccagc atcgtctccg aagccgacaa
      721 gaagggcgac atcctctgcg tgaacgagaa gttcctcgag gtgtccaagt actcgcagca
      781 cgagctcatc gggcacgggc acaacacgac gcgccacccc gacatgccca aggaggtgtt
      841 caagcagatg tgggcgacga tcggccgcgg cgacatcttc cgcggggtcg tcaagaaccg
      901 cgcgaaagac ggcacgccct attacgtcga cgcggtcatc gcgccgatcc tgggcgagaa
      961 cggcaagccg atgaagtacc tcggcgtgcg ctacgacatc accgaagccg agatcgagcg
     1021 ccagaacgcg cgcggcgtga tcgcggcgat cgacgcgtcc tatgcgttca tcgaattcaa
     1081 cctcgacggc acggtcatca aggccaatga acacttcctg agcgcgctgg gctaccggct
     1141 cgacgagatc cagggcaagc accaccgcat gttcgtcgag cccgccctgg catcgtcggg
     1201 cacctactcg cagttctggc gtgacctgaa cgacggcaag gctcagaagg atgtgttcaa
     1261 gcgcatctcc aagaccggcc aggaagtctg gatacaggcg gtgtacgcac cggtcaagga
     1321 cgagatgggc cgcgtgttca aggtcatcaa gatcgccacc gacgtgaccg ccgaggtcaa
     1381 cgccaacaac atgctgcgcc aggctgtcga gcaggcacag caggtcactg ccgcagccaa
     1441 gggaggcgac ctggctcaac gcatcccgct cgaaggcaag agcggcccga tcggcgaact
     1501 gtgcgccggc gtcaattcgc tgatggaaac cacctcggtg atcttcgacg acgtgggccg
     1561 ggtcttcggc gcactggctg agggcgatct ctcgcaacgc atcacacgcg actacgacgg
     1621 catcttcgcc caggtcaaga acgacgccaa cgccaccagc gagaagctcg ccagcatcat
     1681 cgaggacgtg ggccgtgtgt tctcggggct cgccaacggc gatctgtcgc agcgcatcac
     1741 ccgccaggcc gaaggcacct tcgaccaggt caagcaggac gccaacgcga gctgcgacaa
     1801 gctcgcgagc atcatcgagg aggtgcgcgc cgcagccgac gccctgaccg gggcggccaa
     1861 ccaggtgagc gccaccgcgc agagcctgtc gcagtcggcc agcgagcagg cctcttcggt
     1921 cgagcagacg accgcgtcga tcgacctgat gtcggcgtcg atcgcacaga acagcgacaa
     1981 cgcccgcgtg accgacggca tggccaccaa ggccagcaag gaagccggtg acggcggaca
     2041 ggccgtcacg cagaccgtga ccgcgatgaa gcagatcgcc cagaagatca gcatcgtcga
     2101 cgacatcgcg taccagacca acctgctggc gctcaacgcc gcgatcgagg cagcgcgcgc
     2161 cggagagcat ggcaagggct tcgccgtggt cgccgccgaa gtgcgcaagc tggccgaacg
     2221 cagccaggaa gccgccaagg aaatcggcga cctcgccggc aacagcgtca gcacggcgga
     2281 gcgtgccggc aagctgctcg acgagatcgt gccgagcatc cagaagacca gcgaactggt
     2341 gcaggagatc gcggctgcct cgcaggagca gagccagtcg gtcacggaga tcggcggcgc
     2401 gatggggcag ctcagcaagg ccacgcagca gaacgcatcg gcgtcggaag agctcgctgc
     2461 cacgtccgag gaactgtccg gacaggccga gcaactgcag cagtcggtcg ccttcttcaa
     2521 tctcggcggt gacgccactc cgcagcgccg aggcaaggcg gactcgggcg gtacgtccga
     2581 acggagggca ccgaactcac cgctgcgcgt ggccaaggcc ggcggggcca gcgccagccg
     2641 gcctgcagcg accggcacgc acagcaattt ccgtccctac tgaggactga gccatgagca
     2701 cgcacgctcc ccagcgcggt ggcccttcgg cggacgaggc cccgtcccag tacctcacct
     2761 tcatgctcgg cgacgaggtg ttcgcgatgg acatccgcac cgtgcgggag atcatccagt
     2821 acgggccgat gacgacggtt ccgctgatgc cggacttcgt gcgcggcgtg atcaacctgc
     2881 gcggcgcggt cgttccggtg atcgacctgc aggcacgctt cggacgaccg gccgccggca
     2941 tcagcaagaa gtcctgcatc gtgatcttcg atgcagtgcg ccaaggcgaa cgtgtcgagc
     3001 tcggcctgct cgtcgatgcc gtcagcgaag tcatcgaaat cagcggggac cagatcgagc
     3061 cgccgccgaa tttcggtgcc tcggtccggc gcgatttcat ccgcggcatg ggcaaggtgg
     3121 ccaaccgctt cgtgatcatc ctcgagcccg acaaggcctt cgacgtgagc gagatggcac
     3181 agctctgcga ggcagcccag gagcccctgg ccgcctgaga cacccctggc tgaaggctcg
     3241 gtgtcgacag cgcaccgagc ctcagcgcgc accttccatc ccggaacaac ccatgcaagc
     3301 actacgcgac gacaccttcc gccgcatcac cgacctgatg tactcggcga tcggcctgtc
     3361 cttcacggac aacaagaagc cgctgatctc gtcacgactg gccccgcgga tacagcgcct
     3421 cggcctcggc ggctacgagg actacctcgc gctgatctcg agcgacgagg acgccggcga
     3481 gttccagatg gcggtcgacc tgctgaccac caacgagacc tacttcttcc gcgagcccgc
     3541 gcactacgac ctgctggagc acgagctgac gcgcagcaag ccgcagcagc tcgcggtgtg
     3601 gagcgccgca tcgtcattcg gcgacgaggc ctacagcacc gcgatgctgc tgtccgatct
     3661 gcagcagctg ggtcggatcg gcagccaatg gaagatcctc ggcaccgaca tcagcgaccg
     3721 cgtgctgcgc agcgcgatcg aggccgtcta tcccgaggac cgcttgcgca acgtcacacc
     3781 ggagcgcctg cggcgccact gcctgcgggg agacggcgca tccgagggcc tgatccagat
     3841 cagcccgaag ctgcgcaagc acgtcagctt cggacagctg aacctgtgcc agccgatcga
     3901 ggacatcggg ccgttcgacg tgatcttcct gcgcaacgtg ctgatctact tcgacgtgcc
     3961 gaccaagcgc gacgtcgtcg accgggtgct gacacagctg cgtcccggag ggttgttctt
     4021 catcggcacc gccgaagggc gcgtggcgtg caacacgccg ctgcaggcgc tggcgcccgg
     4081 cgcgttccgc aagccctccg cctgagctgt gacaggcgac tcacgatgaa accgatgatc
     4141 ctggaaccgc tggggcaact gcacacgctg catccgggcg atgtcgtctg cgcggagcga
     4201 ggcgagcgga tggagacgct gctgggctcg tgcgtggcga tcgtgctcac cgatccgcgt
     4261 cgcacgctgg gcgcgatgtg ccacatcgtg cacgccagcc gggccacagc cggcgtcgcc
     4321 gagaccggcg cgtacgccga ggtcgcgctc gacacgatgt acgcgctgct gcgcgagcgc
     4381 ggcatcgacc cgcggcaatg cgaggcctat gtctacgggg gcggaaacat gttcccgcaa
     4441 ctgttcaacc agaccgcgca cgtcggcgaa cgcaattccc gatgggcgct cgatgcactg
     4501 gccgaagacg gcgtgcgcgt gctgttccac gacctcggcg gcgataccta ccgccgcctg
     4561 tcgtggaccg tcggcccgaa tgcaccgcag gtcactgcgg tgccggtttg aggaaaccac
     4621 tttcatggca atcagggtgt atgtcgtcga tgactcggcc gtggtgcgtc agacgctgat
     4681 gcacctgctg aaaggcgacg ccgagatcga gctgatgggc agtgcgccca atccactgat
     4741 cgccggaccg gtgatccgca aggaccgtcc ggatgtgctg ctgctcgaca tcgagatgcc
     4801 gggcatggac ggcctcacgt tcctgcgcca gatcatggag gagtcgccga tcccgacggt
     4861 gatctgctcg acgctgacca ccgacggcag ccgcatcgcg ctcgaagcgc tggcagccgg
     4921 cgcggtggcg gtggttgcca agccccgcct gggactgaag cagttcctgg aggactcgcg
     4981 gcgcgaactt ctgcagacgc tgaagacggc cgcccgctcg cgaccgcgcg cgggcatgtc
     5041 ggcgccgcga agcacctctg cgacaaccac cgcgccgaag ccgccgattg ccggcctgca
     5101 tgcactgtcg gtcaacaagc cggtggtgat cggcagttcg acgggcggca cgcaggcgct
     5161 cgagatggtg ctgaccgcgc tgcccggaga cagtccgggc atcgcgatcg tccagcacat
     5221 gccggagaag ttcacggcga tgtacgcgca gcgactcgac ggcatttgcg cgatgaacat
     5281 ccgcgaggcc aaggacggcg accggctcga acgcggcgtc gtgctgatcg ctccgggcgg
     5341 gcgccacatg cagctgcgca aggcgggcgg tcagtatttc gccgtggtgg ccgacggccc
     5401 gcccgtgaac cggcacaagc cttcggtgga cgtgctgttc cgctcggcgg ccgattgcgc
     5461 cggcaaggac ctgctcgcac tcatcctgac cggcatgggc gacgatggcg cacgcggcat
     5521 gaagctgctg cacgaccggg gtgcgcgcac catcgcgcag aacgaggaaa cctgcgtcgt
     5581 cttcggcatg cccaaggaag cgatcaagct cggaggcgtc gacgagatcc tgcccctgca
     5641 gcagatggcc gctgcgatcc ggtccttcga cgcccgcggt tgacggccgc acaaccaccg
     5701 agccaccccg cgagcgccga ggccctgtcg gaaccggctg tcagcgcggc ctgcacctga
     5761 agcgttgagt cagggtgtga accattgctc gatggaaccg gatgcggtgt tgcggagtcc
     5821 cctcgaacag aggttttccg aggttgcatg cgtgcccagg gcatacaaat tgctggtact
     5881 gtgagcacga cttcttgaaa ttgaggtgct gttcgccggc accttttgac ttcgcacctg
     5941 gaggaatgga acaagctggt acccgtcggt gttgatatcg cggaaagctc tgatggagcc
     6001 ctgaagttag gacttcatca gaacttcccc agacgatgac gcagcccgtc gctgagtcat
     6061 cgcctcctag acagacagga gatcgacatg gacgtgatca gtcatttcgc cgcccgattc
     6121 gagcgcacgc gcgaagagga actctcgatc gaggactacc tcgccgagtg caagcgcaac
     6181 cgggtggcgt acgccactgc ggccgagcgc atgctgcagg ctatcggcga gccgcagacg
     6241 gtggacacgc gcaacgaccc gcgcctgtcc cggctgttcg ccaacaaggt catcaagatc
     6301 tatcccgcct tcgccgagtt ctacggcatg gaagactcca tcgaacaggt ggtcagctac
     6361 ttccgccacg cggcacaggg cctggaagaa aagaagcaga tcctctacct gctgggcccg
     6421 gtgggcggcg gcaagagcag catcgccgag cgcctgaagc agctgatgca ggaggtgccc
     6481 ttctacgcga tcaagggctc gccggtgaac gagtcgccgc tggggctgtt cgacgctgcc
     6541 gaagacgggc cgatcctcga gaaggaatac ggcattccgc gtcgctacct gaaccggatc
     6601 ctgtcacctt gggccgtgaa acgtctggag gagttcggcg gcgacatccg caagttcaag
     6661 gtcgtcaagc gttatccctc agtgctcaag caggtcggca tcgccaagac cgagccgggc
     6721 gacgagaaca accaggacat ctcctcgctg gtcggcaagg tcgacatccg caagctcgag
     6781 accttcgcgc aggacgaccc ggacgcctac agcttctccg gcggtctgtg cctggccaac
     6841 cagggcctgc tcgagttcgt cgagatgttc aaggcgccga tcaaggtgct gcacccgctg
     6901 ctgaccgcga cccaggaagg caactacaaa ggcactgaag gcttcggcgc gattccattc
     6961 gatggtgtgg tgctggcgca cagcaacgaa tccgagtgga ccgcgttccg caacaacaag
     7021 aacaacgagg ccttcctcga ccgcatctac atcgtcaagg tgccgtactg cctgcgcgtc
     7081 agcgaagagg tgaagatcta cgagaagctg ctgcgcggct cgtcgctcgc caacgccacc
     7141 tgcgcgccgg gcacgctgaa gatgatgagc cagttcgccg tgctcacgcg actgaaggag
     7201 ccggagaact cgtcgctgta ttcgaagatg ctggtgtacg acggcgagaa cctgaaggac
     7261 accgacccgc gcgccaagag cttccaggag taccgcgact acgccggtgt cgacgagggc
     7321 atgagcggca tctcgacgcg cttcgcgttc aagatcatct ccaaggtctt caacttcgac
     7381 agcaccgagg tcgccgccaa cccggtgcac ctgatgtacg tgctcgaaca gcagatcgag
     7441 cgcgagcagt tcccgcccga gacggagcag aagtacctct cgttcatcaa ggagcacctg
     7501 gcgacgaagt acgcggagtt catcggcaag gagatccaga ccgcctacct cgagagctac
     7561 agcgagtacg gccagaacat cttcgaccgc tacgtcacct acgccgacta ctggatccag
     7621 gaccaggagt accgcgacac cgacaccggc gaggtgttcg accgcgccag cctgaacggc
     7681 gagctcgaga agatcgagaa gcccgcgggc atcgccaacc cgaaggactt ccgcaacgag
     7741 atcgtcaact tcgtgctgcg ggcccgcgcc aacaaccagg gcaacaaccc ggtgtggacc
     7801 agctacgaga agctgcgctc ggtgatcgag aagaagatgt tctccaacac cgaggagctg
     7861 ctgccggtga tcagcttcaa cgccaaggcc agcgccgacg aggccaagaa gcacgaggac
     7921 ttcgtcaccc gcatggtcgc caagggctac acgccgaagc aggtgcgcct gctgtgcgag
     7981 tggtacctgc gggtgcgcaa gtccagctaa ggcaacccga agctccggaa gcacctcatg
     8041 ttccagcaga tcatcgaccg gcgattggca ggcaagaaca agtcgatcgg caaccgcgag
     8101 cgtttccttc gacggcacaa ggagcagatc cgcgaggccg tcaagcgtgc ggtcgatggc
     8161 cgcggcatcc gcgacctggc gcagggcgag gacatccaca tcccgaagcg cgacatcagc
     8221 gaaccggtgt tcggccatgg acagggcggc aagcgcgaga tggtgcatcc gggcaaccag
     8281 gagtacgtgc gcggtgaccg catcgaacga cccaagggcg gtggtggcag cggaagtggc
     8341 ggcggccagg ccagcgactc cggcgagggt gaggacgact tcgtctttca cctgagcaag
     8401 gaagagttca tgcaggtctt cttcgacgac ctggccctgc ccaacctcgc acgcaccacg
     8461 ctgtccgaga cgcccgagtt caagacgcac cgcgccggct acagcagcga cggcaacccg
     8521 agcaacctgc acgtggtgcg atcgatgcga ggcgccatcg gccggcgcat cgcgatcggc
     8581 agcgaatcgc ggcgcgaact gcaccggctc gaggcgcacc tcgaacacct gctgaggcac
     8641 ccggagccga atccgcacgc gcacgagatt ccgccgttgc gactcgagat cgaggccctg
     8701 cgcacacggc tcggtcgcat cccctacctc gacccgatcg acctgcgcta ccgcagccgc
     8761 gtgcgcgtgc cgatgccgac ctcgaaagca gtgatgttct gcctgatgga tgtttccggc
     8821 tcgatggacg aggctcgcaa ggacctgtcc aagcgtttct tcatcctgct gtatctcttc
     8881 ctgacccggc actacgagaa gatcgacatc gtcttcatcc gccatcacac gcaggcccag
     8941 gaagtagatg aacagaactt cttccattcg accgagactg gcggcacggt ggtttccagc
     9001 gcactggtcc tgatggaaga gatcgcgcgg gcgcgctacc cgaccgccga atggaacatc
     9061 tacggcgcgc aggccagcga cggtgacaac tggcaccacg acagcggccg ttgccgagac
     9121 atcctcgcca acaagatcct gccgctgtgc cgctactttg cctatgtgca ggtggcggag
     9181 gaagagcaga acctgtggga cgagtacgcc gcgctggccg ccgaaagcaa ggctttcgcg
     9241 gtgcgcaagg ccaccgacgc gtcgcagatc tatccggtgt tccgcgacct gttcaggaag
     9301 gaaggggttc ccgcatgagc acggcaatca cgcccccctg gggcgaggac gggcggcacg
     9361 agcagcacag cggcggcttg cgacggcgtg cggccgacct gatcgcgccg ccacgcagca
     9421 aggaacccct gccgtcgccc aacgactggt cgttcgagct gatcgatcgt taccacgagg
     9481 tcatacgcga gaccgccgag agcttcggcc tcgacaccta tccgaaccag ctcgagatca
     9541 tcaccgccga gcagatgatg gacgcctacg cgtcggtcgg catgccggtg aactaccgcc
     9601 actggagcta cggcaaggaa ttcatctcga ccgagaagaa ctaccgccgc ggccacatgg
     9661 gactggccta cgagatcgtc atcaactcca acccctgcat cagctacctg atggagg
//
LOCUS       DAOORV010000126         9706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8711_length_9706_cov_9.938556, whole genome shotgun sequence.
ACCESSION   DAOORV010000126 DAOORV010000000
VERSION     DAOORV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8711_length_9706_cov_9.938556"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKV56_14220"
     CDS             <1..447
                     /locus_tag="PKV56_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOX69016.1"
                     /translation="TTPGPLIMVVAFVGFVGGWLKQVFGPEALFLGGAVAAAVATFFT
                     FLPSFVFILAGGPLVEATHGRLGFTAPLSAITAAVVGVILNLALFFAYHVLWPQGFGG
                     HFDAVSAVIAAAAAVALFRFKVGVMPLLGACALVGLAVTLLAPGLR"
     gene            470..883
                     /locus_tag="PKV56_14225"
     CDS             470..883
                     /locus_tag="PKV56_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOX69017.1"
                     /translation="METLPIRLLPGQDLRRSLETAVAERGCRAAFVLSGIGSLSPAVL
                     RLAGASEVRTMTEDVEILTLAGSIAQHGSHLHASVSDARGAVVGGHLAYGCLVRTTAE
                     VLLALLPDWTFTREPDAATGWDELVVRRAAAGPGG"
     gene            912..2093
                     /locus_tag="PKV56_14230"
     CDS             912..2093
                     /locus_tag="PKV56_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX69018.1"
                     /translation="MDRVDCIVIGAGVVGLACARAIAQAGRGVVIVEREPAFGTGVSA
                     RNSEVVHAGLYYPTGSRKARWCVRGAQLLYDYCARRGVAHRRCGKLLVATRDADIAAL
                     EALRLQGIANGVDGLARLTRAEAQALEPELDCVAALSSPASGIVDSRGLMTALLGDAE
                     RAGAVLAVASAVAGARREGDVWRVRVRAGHASGRTPSSSGTEDFELQAHWIVNAAGLD
                     AQSVAASIDGLPATAIPARHLAKGHYFSLTGRAPFSRLIYPMPTDGGLGVHLTLDLGG
                     QARFGPDVDWIGDIAAAGQPGHALDYSVDARRAEAFASEVRRYWPGLGDGALAPTGSG
                     IRPKLSRPGEAAADFRVDGPADHGCPGTVQLFGIESPGLTASMAIAEFVSGIVVQAAA
                     A"
     gene            2297..3397
                     /locus_tag="PKV56_14235"
     CDS             2297..3397
                     /locus_tag="PKV56_14235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX69019.1"
                     /translation="MSVPPGLSGLLPADFNPAGGAEPPFRRRRTDPSFISGFPQPALD
                     AAAAAGLVPPSLLIVDDDPGIVQVLGHMLEGLGHLRFALSAVDALRLARESAPDVVLV
                     DAEMPGMDGFEFCSAMKADPQLSEVPVIFVSAHGHVETEVACFAVGAADFIRKPPTAE
                     VVVARVRTQLRLKSLSDALRRAALVDGLTGVANRRRFDEQLQTECDRALRSGDPLSLL
                     MIDVDQFKRFNDLYGHPAGDACLRSVAGAIQSLVRRPADQVARYGGEEFVMLLPQTEP
                     SGAAHLGQRVVDAVAALRIPHEGSPPLRLVTVSVGVATARCSSDGGCTPATPEALVSA
                     ADQALYAAKSAGRGQSHTYAPSTASIPLSYSI"
     gene            3413..8308
                     /locus_tag="PKV56_14240"
     CDS             3413..8308
                     /locus_tag="PKV56_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX69020.1"
                     /translation="MSDLGAAPHRRWVPVVVLLVGLALSVVAAQWQSRKNNVRVRMAM
                     QTAADGMAEQLLERLQLYEYGLRGARGVLLAAGIDRIDRKTFRQYHESRDIEHEFPGA
                     RGFGFIRRVPADGESAFLAQLRRDAPADIGIRQIEPHGGDRYVIQNIEPIERNLAAVG
                     LDIASEANRRAAADEAARTGRAVITRPITLVQAPGQSQRSFLLLLPVYRPGLPLVSLQ
                     DRQAALVGWTYAPLLIDEVLRDVDLSQARFALALDELTDRTERFFESQAPQGPSAPLA
                     AGDSLQIERHVYGRLWRLSLQPRPLFYAQLHLLAPGSVFATGLLVTLLSSALLLVYQA
                     AHRRRVEMLAGQQRLAALTEQASDAIVVENIDGVVTSWNRAAERLFGWSAGEAIGCHG
                     SDLLLPPAGQAADEALRARVIAGETPEPVESVGLHRHGRPLALSMAVSAILAPDGRVV
                     GVGRLIRDIHAEKEAALSREQFTHRLEIEVGERTASLELARRDLRTVLDALPSMVGYW
                     DRDMRNRFANHAYARWFGVEPSTMPGKHLRDLLGDSLFEMNRPRAEAALRGEPQTFER
                     AVPRPDGSGYRQSLFHYLPDVIDGEVRGFYVLVHEVTEIHEGRRKLADALREIAILLN
                     TVRVHALFSQIDLQGHVIEINDRYCELSGYDREELLAKGFSLLNSGVHPPEFWAEMWR
                     SLRDGKAWRGQICNRAKDGSLFWLDSIFAPIVGADGRVDRYIGIANDITASKNAAADL
                     ANERQRLDNILRGTNVGTWDWNVRTGETRFNERWAEMVGYSLADLAPIGSDFWMRNIH
                     PDDLARSAEALKRHFDGELEQYECEIRMRHRDGHWIWVLDRGRVSSRTPDGQPEWMHG
                     TYQDITERHAAYEALRAATRAAEAASAAKSSFLANMSHEIRTPLNAVIGLTGLLENSR
                     LDAEQSGFVRKIQLASRLLMGLINNVLDLAKIEAGETALDVRPFDLHDLIEGLAAVFG
                     SQAQAKGLDFTVSLPPGLPRRLKGDALRLGQVLTNLLGNAIKFTERGSVALIVQSLGS
                     DDPNQLSVRFAVKDTGIGIDPELHESLFQPFAQADVSTTRRYGGTGLGLSIVKHLTDL
                     MGGRSGVTSQPSQGSEFWITLPLQRVSDEGRGGDSNEVLRLLLLEGNPPRRLGWLEPL
                     AAAFGWRADVVDGVAAATQRLQPDAGGDGQPHVMLVAGGEALAPLGWQALLNTVAAID
                     AQRRPVTLRVGGPEDDSRSEEARQFDSLLPGPADAAALFNAVDAAVRRCGGDVAKVIQ
                     ATRLQGAGVLWLSGVRVLVVDDSDINREVAARVLQREGAIATTAAAAVDALELLARSP
                     GDFDIVLMDVQMPGMDGNEATRRMRTELGLTQIPVIALTAGALLAQRQRALDAGMDDF
                     LSKPLDRDVLIRAVRRHVERVRQAPLPVCMRTEGEPGSPQSPWPEILGIDESGLRERF
                     GDDLALYRRVLRRLCEEFADLAAGDSVPVVPADRLSCAARMHKLYGSAGLLGAHVVAT
                     AAVAAEHALRADVSGAQRGAQAEPEAALDAVRIALKQLLQAAQAWLAEASDSPVEEPP
                     SGCVEPVDSKTLNELRSLLRSQDLAALGRCADLGPALRRSWGAERYALLQSAVERLEF
                     RAALDLVARFEREPAAENVAD"
     gene            complement(8324..>9706)
                     /locus_tag="PKV56_14245"
     CDS             complement(8324..>9706)
                     /locus_tag="PKV56_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HOX69021.1"
                     /translation="LFYKAFPIHVGIIRATTADADGNLTMEREALTLEALAIAMAAHN
                     SGGVVIAQVERLAERGSLNPRQVKIPGVLVDCVVVAEKPEHHMQTFVTQYSAAFAGEI
                     RVPANTLAPMAMGERKLIARRAALELHRNAVVNLGIGMPEGVADVAAEEEVIDLLTLT
                     AEPGVIGGIPASGLNFGAAVNTQAVIDQPSQFDFYDGGGLDIAVLGLAQADAQGNLNV
                     SKFGPRLAGAGGFINISQSAQSVVFVGTFTAGDLQVRIEGGKVHIDSEGTLRKFVREV
                     EHRTFSGERARKNGQRVIYVTERCVFRLAEGGGLELIEIAPGIDIDRHILSQMDFKPA
                     ISPQLRLMDPALFAEAPMKLRERMLALPLSERIDFDERSQMLFINFEGLAIDSARDID
                     AIESEVERRVAPLNKRVDVVVNYDHFSIRPDLMEAYSLMVQRLADRYYAGVRRYAASS
                     FVKARLDSAT"
BASE COUNT         1371 a         3287 c         3434 g         1614 t
ORIGIN      
        1 accacgccgg ggccgctgat catggtggtc gccttcgtcg gcttcgtcgg tggctggctc
       61 aagcaggtgt tcggccccga ggcgctgttc ctgggcggtg ccgtggcggc cgcggtggcg
      121 acgttcttca ccttcctgcc ctcgttcgtg ttcatcctgg ccggcgggcc gctggtcgag
      181 gccacgcacg gcaggctcgg gttcaccgcg ccgctgtcgg cgatcacggc cgccgtggtc
      241 ggggtgatcc tgaacctggc gctgttcttc gcgtaccacg tgctgtggcc gcagggtttc
      301 ggcggccact tcgatgcggt gtcggccgtg atcgcggcgg cggcggccgt ggcgctgttt
      361 cgcttcaagg tcggcgtgat gccgctgctc ggtgcctgcg cgttggtggg cctggccgtc
      421 acgctgctgg cgcccggcct gcgttgagcg acgccgccgc ctgacccgca tggagacgtt
      481 gccgatccga ctgctgcccg gccaggatct gcgccggtcg ctcgagacgg ccgtggccga
      541 gcgcggttgc cgcgcggcct tcgtgctgtc gggcatcggc agcctgtcgc cggcggtgct
      601 gcggctggcc ggcgcgagcg aggtcaggac gatgaccgag gacgtcgaga tcctgaccct
      661 ggccggaagc atcgcgcagc acggttcgca cctgcacgcc agcgtgtccg atgcgcgtgg
      721 tgcggtcgtg ggcggtcacc tggcctacgg ctgcctcgtg cgcacgaccg ccgaagtgct
      781 gctggccctg ctgccggact ggaccttcac ccgcgagccc gacgcggcga ccggctggga
      841 cgaactggtc gtgcggcgcg cggccgccgg tcctggcggc tgagggccgc cccgagcact
      901 gcgcctggcc gatggaccgc gtcgattgca tcgtcatcgg agccggcgtc gtcggtctgg
      961 cctgtgcccg ggcgatcgcg caggcgggcc gcggtgtcgt gatcgtcgag cgcgagccgg
     1021 cgttcggcac cggggtctcg gcgcgcaaca gcgaggtcgt ccacgccggt ctctactacc
     1081 ccacgggcag tcgcaaggcc cggtggtgcg tgcgcggcgc gcagttgctg tacgactact
     1141 gcgcacggcg cggcgtggcc catcggcgct gcggcaagct gctggtggcg acgcgcgatg
     1201 ccgacatcgc agcgctcgaa gcgctgcgcc tgcagggcat cgccaacggg gtcgacggcc
     1261 tcgcgcggct gacccgcgcc gaggcgcagg cgctcgaacc cgaactcgac tgcgtcgcgg
     1321 cgctgtcgtc gccagccagc ggcatcgtcg acagccgcgg cctgatgacc gcgctgctcg
     1381 gtgacgctga acgcgccgga gcggtgctgg cggttgccag cgcagtcgcc ggtgcgcggc
     1441 gcgagggcga tgtgtggcgg gtccgggtca gggcagggca tgcgtccggg cgcacaccgt
     1501 cgtcgtcagg aacggaggat ttcgagttgc aggcgcactg gatcgtcaat gcagccggac
     1561 tcgatgcgca gtcggtggcg gcgtcgatcg acgggttgcc tgcgacggcg atcccggcgc
     1621 gccacctcgc gaaggggcac tatttctcgc tgaccggccg cgcgccgttc tcgcggctga
     1681 tctatccgat gccgaccgac gggggtctcg gcgtgcacct gacgctggac ctcggcgggc
     1741 aggcccgctt cggtcccgac gtcgattgga tcggcgacat tgcggcagcc ggccagcccg
     1801 gccatgcgct ggactactcg gtggatgcgc gtcgcgccga ggccttcgcg tccgaagtgc
     1861 gccgctactg gcctgggctg ggcgacggcg cactggcccc gaccggcagc ggcatccgtc
     1921 ccaaactgag ccgaccgggc gaggcggcgg ccgacttccg cgtcgacggg cctgcagatc
     1981 acggctgccc gggcacggtg cagctgttcg gcatcgagtc gcccggcctg acggcgtcga
     2041 tggcgatcgc cgaattcgtc tcgggcatcg tggtgcaggc cgctgccgcc tgaacttgcc
     2101 gagggctgtc gccaagggaa agcgatgcct tcagcccgca ttcgtgacga ttcggccgct
     2161 ttgttctgcc ggacaatcct gtcataacaa tgtcatttcg catggtgtca gaccaacttg
     2221 tcatgtctgc gggttcagcc ggtgtcgcac gggtccgtgc tcgcgccggt ttccgcctgg
     2281 gctcggtgag gcactgatgt ctgttcctcc cggcctttcc ggtctgctgc ccgccgattt
     2341 caatccggcc ggcggtgcgg agccaccatt ccgccgccgc cgcaccgacc cgtcgttcat
     2401 cagcgggttt ccccagcctg cgctcgatgc cgccgcggcc gccgggctgg tgccgccctc
     2461 gctgctgatc gtcgacgacg atccgggcat cgtgcaggtg ctgggtcaca tgctcgaggg
     2521 gttgggccac ctgcgctttg cgctctccgc cgtcgatgcg ctgcgcctcg cgcgtgaatc
     2581 ggcacccgac gtggtgctgg tcgacgccga gatgccaggc atggatggat tcgagttctg
     2641 ttcggccatg aaggccgacc cgcagttgtc cgaggtgccg gtgatcttcg tcagcgccca
     2701 cggccacgtc gagaccgaag tggcctgttt cgcggtgggc gccgccgact tcatccgcaa
     2761 gccacccacc gccgaggtgg tcgtcgcgcg cgtgcgcacg cagcttcgac tgaagtcgct
     2821 gtccgatgcc ttgcgccgcg ctgcgctggt ggatgggctc accggggttg ccaaccggcg
     2881 ccgcttcgat gaacagctgc agaccgaatg cgaccgtgct ttgcgcagcg gagatccgct
     2941 gtcgctgctg atgatcgatg tcgaccagtt caagcgattc aacgatctct acggacaccc
     3001 ggcgggcgac gcctgcctgc gcagtgtcgc cggagcgatc cagagcctgg tgcgtcgccc
     3061 ggccgatcag gtcgcgcgct acggtggcga ggagttcgtg atgctgctgc cacagaccga
     3121 accctcggga gcggcacacc tgggccagcg cgtcgtcgac gcggtggctg ccttgcggat
     3181 cccgcacgaa ggctcacctc cgctcaggct ggtcacggtc tcggtcggtg tggccacggc
     3241 ccgctgcagc tccgacggcg gctgcacgcc cgccacgccg gaagcgctgg tgagcgcggc
     3301 cgatcaggcg ctctacgccg ccaagtcggc aggccgcgga cagagccaca cctacgcccc
     3361 gagcaccgcc tccattcccc tgtcgtacag catctgatcg ggcatgcggc cgatgtctga
     3421 cctcggtgcc gcgccgcacc ggcgctgggt gccggttgtc gtgctgctgg tcgggctggc
     3481 gctttcggtg gtggctgcgc agtggcagtc gcgcaagaac aatgttcgcg tgcggatggc
     3541 gatgcagacc gcagccgacg ggatggccga gcagttgctc gaacgcctgc agctctacga
     3601 gtacggattg cgtggcgcac ggggggtgct gctcgccgca ggcatcgatc gcatcgatcg
     3661 caagacgttt cgccagtacc acgagtcacg ggacatcgaa cacgagttcc ccggtgctcg
     3721 cggcttcgga ttcatccggc gcgtaccggc cgatggcgag agcgcgttcc tcgcgcaact
     3781 gcgacgggat gccccggccg acatcggcat acgccagatc gaaccgcacg gcggcgaccg
     3841 ctatgtcatc cagaacatcg agcccatcga gcgcaacctg gctgcggttg gactggacat
     3901 cgcgtccgaa gccaatcggc gcgcggcagc cgacgaggct gcacgcaccg gccgggccgt
     3961 tatcacgcga ccgatcacgc tggttcaggc gccgggtcag tcgcagcggt cgttcctgct
     4021 gctgctgccg gtgtatcgcc cgggcctgcc tctcgtcagc ctgcaggatc gccaggctgc
     4081 gctggtgggc tggacctacg cgccgttgtt gatcgacgag gtgctgcgcg acgtcgacct
     4141 gagccaggcg cgcttcgcgc tcgcgctcga cgagctcacc gaccgcaccg agcgattctt
     4201 cgaatcgcag gctccgcagg gcccgtccgc accgctggcc gcaggagatt cgctgcagat
     4261 cgagcgccat gtgtacgggc gcctgtggag attgagcctg cagccccggc ccctgttcta
     4321 cgcgcagctg cacctgctgg ctcctggcag cgtgttcgcc accggcctgc tggtgacgct
     4381 gctgtcatcg gcgctgctgc tcgtctacca ggcagcgcac cggcgccgtg tcgagatgct
     4441 ggccggccag cagcgcctgg ccgcactgac cgagcaggcc agcgatgcga tcgtggtcga
     4501 gaacatcgac ggtgtcgtca ccagctggaa ccgcgcggcc gagcgcctgt ttggctggag
     4561 cgccggcgaa gccatcggat gccacgggag tgatctgctg ctgccgcccg ccggccaggc
     4621 tgcagacgag gcgctgcgcg cgcgcgtgat cgccggcgag acgcccgagc ccgtcgagtc
     4681 ggtcggactg catcgccacg gcaggccgct ggcgttgtcg atggcggtct ccgcgatcct
     4741 ggctcccgac ggccgggtgg tcggagtcgg tcgcctgatc cgcgacatcc acgccgagaa
     4801 ggaggctgcg ctgtcgcgcg agcagttcac gcaccggctg gagatcgagg tgggcgagcg
     4861 caccgcctcg ctcgaactgg cgcgccgcga cctgcgcacg gtgctcgacg cgctgccttc
     4921 gatggtcggc tactgggatc gcgacatgcg caaccgcttc gccaatcacg cctatgcccg
     4981 gtggttcggg gtcgagccct cgacgatgcc gggcaagcac ctgcgcgacc tgctgggcga
     5041 cagcctgttc gagatgaatc gcccgcgcgc ggaagccgcc ctgcgcggcg agccgcagac
     5101 cttcgagcgg gccgtcccga ggccggatgg atcgggctac cggcagtcgc tgttccacta
     5161 cctgcccgat gtgatcgatg gcgaagtgcg tggcttctat gtgctggtgc acgaggtgac
     5221 cgagatccac gagggtcgcc gcaagctggc tgacgcgctg agggaaattg ccatcctgct
     5281 caacacggtg cgggtccatg cgctgttctc gcagatcgat ctgcagggcc atgtcatcga
     5341 gatcaacgac cgctactgcg agttgagcgg ctacgaccgc gaggagctgc tcgccaaggg
     5401 tttcagtctg ctcaattcgg gcgtgcaccc gcccgagttc tgggccgaga tgtggcgcag
     5461 tctccgggac ggcaaggcgt ggcgcggcca gatctgcaac cgggcgaagg acggctcgct
     5521 gttctggctg gacagcatct tcgcgcccat cgtcggcgcc gacggtcgcg tcgatcgata
     5581 catcggcatc gccaacgaca tcacggccag caagaacgcg gcggccgatc tggcgaacga
     5641 gcggcagcgg ctcgacaaca tcctgcgcgg aaccaacgtc ggcacctggg actggaacgt
     5701 ccggaccggc gagacacgct tcaacgagcg ctgggccgag atggtcggct attcgctggc
     5761 cgatctcgca ccgatcggca gcgacttctg gatgcgcaac atccatcctg acgatctggc
     5821 gcgctcggcc gaggccctga agcggcactt cgacggcgaa ctcgagcagt acgaatgcga
     5881 gatacgcatg cgacaccgcg acggccactg gatctgggta ctcgatcgcg gccgcgtgtc
     5941 gagccgcacg ccagacggcc agccggagtg gatgcacgga acctaccagg acatcaccga
     6001 gcgacatgcg gcctacgagg cgctgagggc ggccacccgc gcggccgagg cggccagcgc
     6061 agccaagagc agcttcctcg cgaacatgag ccacgagatc cgaacaccgc tcaacgccgt
     6121 gatcgggttg accggcctgc tcgagaacag ccgcctcgat gccgagcaaa gtggcttcgt
     6181 gcggaagatc cagctggcca gtcgattgct gatgggactc atcaacaacg tgctggatct
     6241 ggccaagatc gaggcgggtg aaaccgctct ggacgtgcgg ccgttcgacc tgcacgacct
     6301 gatcgagggc ctggcggccg tgttcggcag ccaggcgcag gccaagggcc ttgatttcac
     6361 ggtgagcctg ccgcccggcc tgccgcgccg actgaagggc gacgcgctgc ggctggggca
     6421 ggtgctgacc aacctgctcg gcaacgccat caagttcacc gagcgtggca gcgtggcgct
     6481 gatcgtgcaa tcgctcggca gcgatgaccc gaaccagctc agcgtgcgtt ttgccgtgaa
     6541 ggacaccggc atcggcatcg accccgaact gcacgaatcg ctcttccagc ctttcgcgca
     6601 ggccgacgtg tccaccacgc gccgctacgg cggaaccggg ctcggcctgt ccatcgtcaa
     6661 gcatctgacc gacctgatgg gcggtcgcag cggcgtgacc agccagccgt cgcaaggcag
     6721 cgagttctgg atcacgctgc ctttgcagcg tgtctccgat gagggcaggg gtggtgattc
     6781 aaacgaagtg ctgaggctgc tgctgctgga aggcaacccg ccccgccgcc tgggatggct
     6841 ggagccgctg gctgcagcct tcggctggcg tgccgatgtg gtcgacggtg tcgcggcagc
     6901 cacgcagcga ctgcagcccg acgcgggcgg tgatggtcag ccccacgtca tgctcgtggc
     6961 cggcggtgag gccctggcac cgctcggctg gcaggcgctc ctgaacaccg tggcggcgat
     7021 cgatgcacag cgccgcccgg tgaccttgcg ggtcggtggc cccgaagacg acagccggtc
     7081 cgaagaggct cgccagttcg acagtctgct tcccgggcct gccgatgcag cggcgctctt
     7141 caacgcggtc gatgcggccg tgcggcgctg cggtggcgat gtggcgaagg tcattcaggc
     7201 cacgcggctg cagggtgctg gcgtgctctg gctttccggg gtgcgggtgc tggtggtcga
     7261 tgacagcgac atcaatcgcg aggtggccgc gcgcgtcctg cagcgtgagg gtgccatcgc
     7321 gaccacggcc gctgcggcgg tcgatgcgct cgagctgctc gcccggtcgc cgggcgactt
     7381 cgacatcgtc ctgatggacg tccagatgcc cggcatggac ggcaacgagg cgacccggcg
     7441 catgcgcacc gaactcgggt tgacgcagat ccccgtgatc gcgctgaccg ctggcgccct
     7501 gctggcccag cggcagcgcg cgctggacgc cggcatggac gatttcctca gcaagccgct
     7561 cgaccgcgac gtgctgatcc gggcggtgcg tcgccacgtc gagcgggttc ggcaggcgcc
     7621 gctgccggtt tgcatgcgaa cggaaggtga gcccggatcg ccgcaatcgc cctggcccga
     7681 gatactgggc atcgacgagt ccgggttgcg cgagcggttc ggtgacgacc tcgcgctgta
     7741 ccggcgcgtt ctgcggcgcc tgtgcgaaga gttcgcggat ctcgcggctg gcgattcggt
     7801 accggtcgtg ccggcggacc ggctgtcttg tgcagcccgg atgcacaagc tgtacggcag
     7861 tgccggattg cttggcgcac atgtcgtcgc gaccgccgct gtggcggccg aacacgcgct
     7921 gcgtgccgat gtgtccggcg ctcagcgcgg cgcgcaggcc gagcccgagg ctgcactcga
     7981 tgcggtgcgc atcgcgctga agcaactgct gcaggccgcg caggcctggc tggccgaggc
     8041 gagcgattca ccggtcgagg aaccgccgtc cggctgcgtc gaaccggtcg attcaaagac
     8101 tctgaacgag ttgcgcagcc tgttgcgtag ccaggatctg gcggcgctgg gacgctgcgc
     8161 cgatctgggg ccggcactgc gtcggtcctg gggcgcggaa cgctacgcgc tgctgcagtc
     8221 cgcggtggag cggctcgagt tccgggcggc gctcgatctg gtggcccgct tcgagcgcga
     8281 accggctgcc gagaacgtgg cggactgacc cgccgcagcg gggtcaggtc gcgctgtcga
     8341 gcctggcctt cacgaagctg ctcgccgcgt agcgcctcac gcctgcgtag tagcggtcgg
     8401 cgagccgctg caccatcagg ctgtaggcct ccatcagatc ggggcggatg ctgaagtggt
     8461 cgtagttgac gaccacgtcc acccgcttgt tcagcggagc cacccgacgc tcgacttcgg
     8521 actcgatcgc gtcgatgtca cgcgcgctgt cgatggccag gccctcgaag ttgatgaaca
     8581 gcatctggct gcgttcgtcg aaatcgatgc gttcggacag cggcagggcc agcatgcgct
     8641 cgcgcagctt catcggtgct tcggcaaaca gcgccggatc catcagtcgc agctgcgggc
     8701 tgattgccgg cttgaagtcc atctgggaca ggatgtggcg gtcgatgtcg atgcccggcg
     8761 cgatctcgat cagttccagt ccgccgcctt cggccagtcg gaacacgcac cgctcggtca
     8821 cgtagatcac gcgctggccg ttcttgcggg cacgctcgcc actgaaggtg cggtgctcca
     8881 cttcgcgcac gaacttgcgc aaggtgcctt cgctgtcgat gtgcaccttg ccgccctcga
     8941 tgcgcacctg caggtcgccc gcagtgaacg tgccgacgaa caccacggac tgcgccgact
     9001 ggctgatgtt gatgaagcct ccggcgccgg cgagccgggg gccgaacttg ctgacgttga
     9061 ggttgccctg cgcgtcggcc tgtgccaggc cgagcaccgc gatgtcgagc ccgccgccgt
     9121 cgtagaaatc gaactggctg ggctggtcga tgaccgcctg cgtgttgacg gccgcgccga
     9181 aattcagccc gctcgccggg atgccgccga tcacgccggg ctcggcggtg agcgtgagca
     9241 ggtcgatcac ctcctcttcc gccgccacgt cggccacgcc ctcgggcatg ccgatgccca
     9301 ggttgacgac ggcattgcgg tgcagttcca gcgccgcgcg ccgcgcgatc agcttgcgct
     9361 cgcccatcgc cattggtgcg agcgtgttcg ccggcacgcg gatctcgccg gcgaaggcgg
     9421 cgctgtactg cgtcacgaag gtctgcatgt ggtgctcggg cttctcggcc accaccacgc
     9481 agtcgaccag cacgccgggg atcttcacct gccgcgggtt cagcgagccg cgttcggcga
     9541 gccgctccac ctgtgcgatc accaccccgc cgctgttgtg cgccgccatc gcgatggcca
     9601 gcgcctccag cgtgagcgcc tcgcgctcca tcgtcaggtt gccgtcggcg tcggcggtgg
     9661 tggcgcggat gatgcccacg tggatcggga aggccttgta gaacag
//
LOCUS       DAOORV010000127         9623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8799_length_9623_cov_7.887228, whole genome shotgun sequence.
ACCESSION   DAOORV010000127 DAOORV010000000
VERSION     DAOORV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_9623_cov_7.887228"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKV56_14250"
     CDS             complement(<1..398)
                     /locus_tag="PKV56_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX69022.1"
                     /translation="MLDNQSILITGGTGSFGRAFVKTVLARYPGVKRVVVFSRDELKQ
                     YEMSQQFSTREHPGMRYFIGDVRDADRLRRALEGIDIVVHAAALKQVPAAEYNPFECI
                     KTNVLGAQNVIDACLDQQVRRVVALSTDKAA"
     gene            complement(455..2158)
                     /locus_tag="PKV56_14255"
     CDS             complement(455..2158)
                     /locus_tag="PKV56_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69023.1"
                     /translation="MKIDALLMLQRSDVAHFVRDPAPYAGLKLIFIDPGTVDEAVNHG
                     VDSARFDYRPLDVGRHLQARVTAEAMNRAQALDRLLYRERVKLFGQGEFQGWDVSVSR
                     LFFVRALMARELGAICDRSFSESSIGVFRPTRPQQFYFDSFVTTDLFTAGSPRWKVVD
                     HYDTTLNWVDDSNASCFDFDQIERRVAAGAAQAVTHIPTVYAQLRHYAREMAQTFSSS
                     IDLPSPFWDIPLQRDRSPLVRIDSVASRFLGDECRTYRERARQVIEAQLRDLLPHPHA
                     LQSQVDLMADRCFMQAVNYQGLSVALKESQPHFVLTEHDTGSIGPLFTVAAQLGSPIT
                     VLPHSSYPTELLPHSTRVVAIERDGFAAPVRTVWGELVATRPVRFAPRKQMLGRPHVA
                     TVCLLLNTMFSQGISHVDLVGLAGFHKALSELCAARGVRLLVRLKPNAAGVMIASGAL
                     GVPVAELQAVLAASLEEIATASDLCVAYGEPTTASIEFMEAGSCLMQVCDQYWPAGYL
                     SCAPYLTAASVPFLSGADALPVIDRLLADAAHFRTVAGRQRQELAARFTAVDGRIFDA
                     R"
     gene            2431..3447
                     /locus_tag="PKV56_14260"
     CDS             2431..3447
                     /locus_tag="PKV56_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX69024.1"
                     /translation="MLEPPLQSAKDLPLGTRPEAAPADRVNPMAPRALLQDMTISAVE
                     PEQAPLPVVLIHGLLSTPREFGLISLPIQSRGVPLIIPNIKGYTEADRSQYVGWKDWV
                     EAACDAIREQVGVDQPFVLGGLCSGGMVAAAVAARGEFNVRTLVMMSPSFGYDGWART
                     IWWRLRGLGYALHLDQWISIKESPPYGIKNEKIRKWVERDMQQRATSAAGPSSLPLWA
                     IHQSEKLMNHVVRQFPRLRIPTVVIHSRLDEICSLGIVKRVFETLPAGLNRLVVLENS
                     YHMITIDNDRQQVTAELLKCSGQRDRIAAAAIETCSITPTPHPAGALTPADSYTGALS
                     CPTP"
     gene            3432..3677
                     /locus_tag="PKV56_14265"
     CDS             3432..3677
                     /locus_tag="PKV56_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX69025.1"
                     /translation="MSNTMTELLGLIQKAYDIDPASIDPHKPLSEFGLDSLALAELMF
                     NIEDHFHIDYPESRTGVQTLAELAQVVDEVRAAVQVK"
     gene            3674..4894
                     /locus_tag="PKV56_14270"
     CDS             3674..4894
                     /locus_tag="PKV56_14270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOX69026.1"
                     /translation="MSARDVVISGLGLVSPHGDDPAIAFEALLRGQSAINLWTREGLP
                     PAAVASAAFSPERWFTKLQLVGVDRVSQMAVAAAEMARTDARWPEGIDPERIGVFIGS
                     GMGGASALEDGYEAARSGRRVSPLTVVAAMTNAPAAHIAIRARAEGPVYTYSVACASS
                     ALAIAEAAKAIAAGEIDIAIAGGAEALLVPGVVRAWQALQTLATPDAADPGRSCRPFD
                     LHRSGFALGEGAAMLVLESRQHAQDRGAPVRVRFAGSGISCDAQHMTKPQAQGQMRAL
                     RNALRASGLSPRDIGYCNAHGTATRVGDGVECEALRTVWGDDIDALRVSSTKSMHGHL
                     LGAAGALEAVVTVLALEQRCAPPTPSCQEPDPACAVPLIRETKASLPDLRSAISNSFA
                     FGGTNVVLAFSRND"
     gene            complement(4938..5870)
                     /locus_tag="PKV56_14275"
     CDS             complement(4938..5870)
                     /locus_tag="PKV56_14275"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOX69027.1"
                     /translation="MNTVTLPRACAHACAILTAALLVACAPQDEPILSPAEAASAPDA
                     RFVAIARGRVDVPGGLLSVASPQGGLIQNWSVQPGDSVRKGQVLAQIDARETRHQLEQ
                     AKSEQGLAAAQLEALRSRIPAARTRLERLQQVQAAGAGSGQAVDDARAVLSEAEKQLV
                     VQRSSLAVVQQRVAQANQMLAAATIRAPVAGRVVQRTTQVGEYIAPYGVLLSLLPEGP
                     RIVRADLNESLIARVRVGMRAEVVSVAEGSAVNGARVLSIGEVFGPPRTVDSADSEVV
                     IDARVVECLLELDKPGLRVGQRVLVRFLPSEPAK"
     gene            complement(5867..6604)
                     /locus_tag="PKV56_14280"
     CDS             complement(5867..6604)
                     /locus_tag="PKV56_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX69028.1"
                     /translation="MTAPPIAIDPGAPVLRGRNLVKSFRSGRIDTPVLRGLSLDVCPG
                     ELTLISGPSGCGKSTLLASLSGLQRPDAGEVEALGALLWSLDARALERFRLHHTGFVF
                     QGFNLFGALSAVEQVMLPLGALKLSRTQARERALEALDEVGLSARSGLRPAEMSGGEK
                     QRVAIARALAKRPQLLFADEPTSALDAVNGQLVIDLLHRLARSHGAAVLCVSHDPRVV
                     AHADRVLHMEDGRLLDDTRPGAAEGHT"
     gene            complement(6601..7737)
                     /locus_tag="PKV56_14285"
     CDS             complement(6601..7737)
                     /locus_tag="PKV56_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX69029.1"
                     /translation="MIVPLARKSLLHEWRRFLPAVVAVAFSGLLLLVQAALVLGIFGS
                     SAVYIDASRGDLWVGHPGTRTVELGRAIPRDTEIWLRLDPAVDRVEAYSWIEGDWRSR
                     PDVGAITLYVSGVDTSPDALLFARVLTPDMRVRLDEPGSVIVDEADMKKLGVAIGDRP
                     RINGRTVRVVGAVSGLRTLGGVNILSSLETISRLQPGGPGDERVPYYVVRLHDAAQLP
                     EARSRLAAAGREHGFEVWTRKEFAKSASDYWLLDTGAGLGVLFLAVVVSLVGAVITSQ
                     TLMGAVAGSATEYAALQALGIGVRALRGVVLEQAAWIGSAGLLLGGVGAWAALSLARH
                     YHVPVVLESWAVVACAVLVLGISMISGLAAVRALRSADPATLLR"
     gene            complement(7734..9140)
                     /locus_tag="PKV56_14290"
     CDS             complement(7734..9140)
                     /locus_tag="PKV56_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX69030.1"
                     /translation="MSVFPCVPSALRIRILGIAVACLAGCASTPAPAPPAAVPVVPLP
                     VAWAAPPLLGDAVDLTSWWRAFGDSALDGLVARALAGSPDLEAAAARVRSAQAMGQEM
                     AARLGPQVSLVGDPQQTANGRSTYFQAGVAARWDVPLQDRQIATRGLAEADEQLALAD
                     EQASRATLVAEVARAYFEMRAAEREQHLLKQLAAEADERERLTRVLLQANQVGADEVQ
                     AAVVAAVQARAATLEPVALAALARTRLGVLVGDLQADLPPPAVDSALPARAPLGLTAL
                     PADLVRQRPGIRRAEHTVLRAAHAAGLARADAQPRISLAGLVGLSAAITGPASGAVRA
                     VLSAGPSFSMSLFDGGAIAASQRARDADFDAATAQYRQAVLQGVAEAQSALLQLDHER
                     HRTVAAVAVEEAVRQRSNATEAQARLGLATGFQRADARSAAVQARRDTLRAGLAEQVA
                     YVALFTAFGGASLPTPSP"
     gene            9399..>9623
                     /gene="flhD"
                     /locus_tag="PKV56_14295"
     CDS             9399..>9623
                     /gene="flhD"
                     /locus_tag="PKV56_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HOX69031.1"
                     /translation="MNADQILTEIREANLSYLMLAQSLIRTDREQALYRLGISEENAA
                     LLQMLTPAQMMKIASGNTLLCRFRMDDDMVW"
BASE COUNT         1674 a         3317 c         3227 g         1405 t
ORIGIN      
        1 gcggccttgt cggtcgacag cgccaccacg cggcgcacct gctggtcgag gcaggcgtcg
       61 atgacgttct gtgcgcccag cacgttggtc ttgatgcact cgaacgggtt gtactcggcg
      121 gccggcacct gcttcagcgc ggcggcgtgc acgacgatgt cgatgccctc cagagcacgg
      181 cgcaggcgat cggcatcgcg cacatcgccg atgaagtagc gcatgccggg gtgctcgcgg
      241 gtcgagaact gctgcgacat ctcgtactgc ttcagctcgt cgcgcgagaa gacgaccacg
      301 cgcttgacgc cggggtagcg tgccagcacg gtcttgacga aggctcgtcc aaacgagccc
      361 gtgccaccgg tgatcaggat ggattggttg tcgagcatgg gacggtccgg aagtgaagtg
      421 ggggacagag gcgaggtcgg cgtcggcgcg tcgatcagcg ggcgtcgaag atgcggccgt
      481 cgactgccgt gaagcgcgca gccagttcct ggcgctggcg cccggcgacg gtgcggaaat
      541 gggcggcatc ggccagcagg cgatcgatga ccggcagcgc gtcggcgccg ctcaggaagg
      601 gaacgctggc agccgtgagg taaggggcgc aggacaggta gccggctggc cagtactggt
      661 cgcagacctg catcaggcag ctgcccgctt ccatgaattc gatgctggcg gtggtgggct
      721 cgccgtaggc cacgcacagg tcggaggcgg tcgcgatctc ctcgagcgag gcggcgagca
      781 ccgcctgcag ttcggccacc ggcacgccga gagctccgga ggcgatcatg acgcccgccg
      841 cgttgggctt cagccggacc agcaagcgca cgccgcgcgc ggcgcagagc tccgaaagcg
      901 ccttgtggaa gcccgccagg ccgaccaggt cgacgtggga tatgccctgg ctgaacatcg
      961 tgttcagcag caggcagacg gtggcgacat gcgggcgccc gagcatctgc ttgcgcggtg
     1021 cgaagcggac cggccttgtg gcgaccagct cgccccagac ggtgcgcacc ggtgccgcga
     1081 agccatcgcg ctcgatcgcg acgacgcgtg tcgaatgcgg caggagttcg gtcgggtagg
     1141 acgagtgagg cagcacggtg atggggctgc cgagctgagc cgcaaccgtg aacagcgggc
     1201 cgatgctgcc ggtgtcgtgc tcggtcagca cgaagtgcgg ctgcgactcc ttcagcgcca
     1261 ctgacagccc ctggtaattg accgcctgca tgaagcaccg gtcggccatc agatcgacct
     1321 gcgactgcaa tgcgtgcgga tgggggagca ggtcgcggag ctgagcctcg atcacctgcc
     1381 gcgcgcgctc gcggtaggtg cggcactcgt caccgaggaa ccgcgaggcc accgagtcga
     1441 tgcgaaccag cggtgagcgg tcgcgctgca acggaatgtc ccagaacggg ctggggagat
     1501 cgatgctcga ggagaaggtc tgcgccatct cgcgggcgta gtgccgcaac tgggcgtaga
     1561 ccgtcgggat gtgcgtgacg gcctgggcgg caccagcggc cacccgacgc tcgatctggt
     1621 cgaagtcgaa gcacgaggca ttcgaatcgt ccacccagtt caacgtggtg tcgtagtggt
     1681 cgaccacctt ccagcggggc gagccggcgg tgaacaggtc ggtggtgacg aaggagtcga
     1741 agtagaactg ctggggtcgt gtcggccgga agaccccgat cgaggactcg gagaagctgc
     1801 ggtcgcagat cgcgccgagt tcgcgggcca tcagcgcccg gacgaagaac aggcgcgaca
     1861 ccgacacgtc ccaaccctgg aactcaccct gtccgaacaa cttgacgcgc tcgcggtaca
     1921 gcaggcggtc gagggcctgc gcccggttca tcgcctcggc ggtcacgcgc gcctgcagat
     1981 gccgaccgac atcgagcggg cggtaatcga agcgcgccga gtcgacgcca tggttgacgg
     2041 cctcgtcgac cgtgccggga tcgatgaaga tgagcttgag gcctgcgtag ggcgcgggat
     2101 cgcgaacgaa atgcgcgacg tcgctgcgct gcagcatcag cagggcatcg attttcatgg
     2161 ggtggggtcg gcagcgtagc ggttcactga agctatttca cgaccgaccg gtcccatcgc
     2221 atcgctggag aaccacgtca ttcgccccgc atttcacccc cgagtggcct gctcctccag
     2281 tcggcggatg gcactgggga cgactccggc agcccggggg gtgctcccgg gaaagacgtt
     2341 aaggcaggct caacccgcac cgattaaact tcggcacggc gttcgactgg tggtcagtgc
     2401 tgtgtctcac acctgacatc atcgaaaatc atgcttgaac ccccgctgca gtcggcgaag
     2461 gacctccccc tcgggacacg ccccgaagct gcccccgccg accgggtcaa cccgatggcc
     2521 ccgcgggcgt tgttacagga catgactatt tccgctgtcg agccggagca ggctccgttg
     2581 cccgtggtgc tgattcacgg cctgctcagc accccccgcg agttcggcct gatctcgctg
     2641 cccatccagt cgcggggcgt cccgctgatc attccgaaca tcaagggcta caccgaggcc
     2701 gatcgaagcc agtacgtcgg ctggaaagac tgggtcgaag ccgcctgcga cgcgatccgg
     2761 gagcaggtcg gcgtcgacca gccctttgtg ctcggggggc tgtgctccgg aggcatggtg
     2821 gcggccgccg tggctgcgcg aggtgaattc aatgtccgca cactggtgat gatgtcgccg
     2881 agcttcggct acgacggctg ggcaaggacc atctggtggc gattgcgcgg gctgggttac
     2941 gccctccacc tggaccagtg gatctcgatc aaggagagcc cgccctacgg gatcaagaac
     3001 gagaagatac gcaagtgggt cgaacgggac atgcagcagc gagcgacgag cgcggccgga
     3061 ccgtccagcc tgcccttgtg ggccatacat cagtccgaaa agctgatgaa ccatgtcgtg
     3121 aggcaatttc cgcggttgcg gatccccacg gtcgtgatcc actcccgact ggacgaaatc
     3181 tgcagtctcg gcatcgtgaa aagggttttc gagacactgc cagcgggctt gaaccgtctc
     3241 gtggtgctcg agaattcata tcacatgatc accatcgaca atgaccgtca acaggtcacg
     3301 gcagaattgc tgaaatgctc cggccagagg gatcgaatcg ccgcggcggc gatcgaaacc
     3361 tgttcgatca cgccgacacc gcatccggcg ggcgcgctca ccccggccga ttcatataca
     3421 ggagcgcttt catgtccaac accatgaccg aattgttggg attgatccag aaggcctacg
     3481 acatcgatcc ggcctcgatc gatccgcaca agcccttgtc cgaattcggg ctcgactccc
     3541 tggcgctggc cgagctcatg ttcaacatcg aagaccactt tcacatcgat tacccggagt
     3601 cacgcaccgg cgtgcagacc ctggccgagc tggcgcaggt ggtggacgag gtgcgtgccg
     3661 ccgttcaagt gaaatgagtg cgcgcgatgt ggtgatttcc ggcctcgggc tggtatctcc
     3721 gcacggcgac gatcccgcga tcgccttcga ggccctgctg cgtggccaat cggccatcaa
     3781 cctctggacc cgcgaagggc tgccgccggc agcagtcgcc agcgcagcgt tctcccccga
     3841 gcgctggttc accaagcttc aactcgtcgg cgtcgaccgc gtcagccaga tggccgtcgc
     3901 ggcggcggaa atggcgcgca ccgatgcccg ctggcccgag ggcatcgacc ccgagcgcat
     3961 cggcgtgttc atcggaagcg gcatgggcgg tgcgtcggca ctcgaggacg gctacgaggc
     4021 cgcccgcagc ggtcgccgag tctcgccgct gacggtcgtt gccgcgatga ccaacgcccc
     4081 ggccgcgcac atcgcgatcc gcgcccgggc cgagggaccg gtctacacct actcggtggc
     4141 ttgcgcttcg tcggcactcg ccatcgccga ggcggccaag gcgatcgcgg ccggcgagat
     4201 cgacatcgcg atcgccggcg gcgccgaggc cctgctggtg ccgggcgtcg tgcgcgcctg
     4261 gcaggcgctt caaacgctcg ccactccgga cgcggccgat cccggtcgct cgtgccgccc
     4321 cttcgacctc catcgctccg gcttcgcgct cggcgagggt gcggcgatgc tggtgctcga
     4381 atcccggcag cacgcgcagg accggggggc accggtgcgg gtgcgattcg cgggcagcgg
     4441 catcagttgc gacgcgcagc acatgaccaa gccgcaggcc cagggacaga tgcgtgcgct
     4501 gcgcaacgca ctgcgtgcca gcggcctgtc gccgcgcgac atcggctact gcaacgccca
     4561 cggcacggcg acgcgggtcg gcgatggtgt cgagtgcgaa gccttgcgca cggtctgggg
     4621 tgacgacatc gacgcattgc gcgtgagttc gaccaagtcg atgcacggtc acctgctggg
     4681 tgcagccggc gctctcgagg ccgtcgtcac cgtcctggcg ctcgaacagc gctgtgcgcc
     4741 accgaccccc agttgccaag aaccggaccc cgcatgcgcc gttccgctga tccgcgaaac
     4801 gaaggcatcg ctgcccgacc tgcgctcagc gatcagcaat tccttcgcct tcgggggcac
     4861 caacgtcgtg ctggccttct cgcgcaacga ctgatcgcgg gaagcacggc tcccggagca
     4921 cgcacccggc ggtgcttcta cttggccggt tcggatggca ggaagcggac cagcacccgc
     4981 tgtcccacgc gcaggcccgg cttgtccagc tcgagcaggc attcgaccac ccgggcgtcg
     5041 atgaccactt cgctgtccgc cgaatccacc gtgcgtggcg ggccgaacac ctcgccgatg
     5101 ctcagcaccc gcgctccatt caccgcactg ccttccgcaa ccgacaccac ttcggcgcgc
     5161 atgccgaccc tgacgcgagc gatcagcgat tcattcaggt cggcacgcac gatgcgcggc
     5221 ccttcaggca gcagactgag cagcacgcca tagggggcga tgtactcgcc gacctgcgtc
     5281 gtgcgctgca cgacgcggcc cgcgaccggc gcacggattg tcgcggcagc cagcatctga
     5341 ttggcctgag ccacccgctg ctgcacgacc gccagcgaac ttcgctggac gaccagctgc
     5401 ttctcggcct cgctcaacac ggcccgcgcg tcgtcgacgg cctgcccgct gccggcgcct
     5461 gcagcctgca cctgctgcag gcgctcgagc cgcgtgcgcg ccgcaggaat gcgggatcgc
     5521 agtgcttcga gctgcgctgc ggccaggcct tgctccgact tggcctgctc cagctggtgg
     5581 cgtgtttcac gggcgtcgat ctgggcaagg acctgtccct tgcgcaccga gtcccccggc
     5641 tgtacggacc agttctggat caggccgccc tgcggactcg cgacgctcag caggccgcct
     5701 ggcacgtcga cccggccacg tgcgatcgcg acaaagcgcg catcgggagc cgacgcggcc
     5761 tcggccgggc tcaggatcgg ttcgtcctgc ggcgcacagg cgaccagcag ggccgccgtc
     5821 aggatcgcgc aggcatgcgc acaggcacgc gggagagtca cggtattcat gtgtgtcctt
     5881 cggccgcgcc ggggcgcgtg tcgtcgagca ggcgcccgtc ttccatgtgt agtacgcgat
     5941 cggcgtgcgc cacgacccgg ggatcgtggc tgacgcacag caccgctgcg ccgtggctgc
     6001 gtgcgagtcg gtgcagcaga tcgatcacca gttgaccatt gaccgcgtcc agtgcgctgg
     6061 tcggctcgtc ggcgaacagc aactgggggc gcttggccag cgcgcgcgcg atcgccaccc
     6121 gctgcttctc cccacccgac atctcggcgg gacgcagtcc cgagcgcgca gacaggccga
     6181 cttcatcgag cgcttccagc gcgcgttcgc gggcctgggt gcgcgacagc ttcagagcgc
     6241 ccagcggcag catcacctgc tcgacggcgc tcagcgctcc gaacaggttg aagccctgga
     6301 acacgaagcc ggtgtgatgc agccggaagc gctcgagtgc ccgtgcgtcg agcgaccaga
     6361 gcagtgcgcc cagtgcctcg acctcgccag catcggggcg ctgcagcccg ctcagcgatg
     6421 caagcaaggt gctcttgccg cagccagacg ggccggagat gagggtcagt tcgcccgggc
     6481 agacatccag gctcaatccg cgcaggaccg gcgtatcgat gcggccgctg cgaaacgact
     6541 tgaccaggtt gcgcccccgc aagaccggcg ccccggggtc gatcgcgatg ggaggggccg
     6601 tcatctcagc agcgtcgccg gatcggcgct gcgcagcgcg cgcaccgccg ccaggcctga
     6661 aatcatcgag atcccgagca ccagcacggc acaggccacc accgcccagc tttcgaggac
     6721 cacaggcaca tggtagtgac gggccagcga caacgcggcc caggccccca ctcctccgag
     6781 cagcaggcca gcgctgccga tccaggccgc ctgctccagc accaccccgc gcaaggcacg
     6841 caccccgatg ccgagcgcct ggagcgccgc gtattcggtc gcggagccag ccaccgcgcc
     6901 catcagcgtc tggctggtga tgaccgcgcc gaccaggctc accacgaccg ccagaaacag
     6961 cacgcccagg cccgccccgg tgtcgagcaa ccagtagtca gacgcactct tggcgaactc
     7021 cttgcgcgtc cagacttcaa atccatgctc gcgccctgcg gcggccagcc ggctccgtgc
     7081 ctctggcagc tgggccgcgt catgcagccg caccacgtaa tagggcaccc gctcgtcgcc
     7141 cggaccgccc ggctgcagcc ggctgatcgt ttccagcgac gacaggatat tcaccccgcc
     7201 cagcgtgcgc agccccgaaa ccgcgccgac gacccgcacg gtgcggccat tgatccgggg
     7261 ccggtcgccg atcgccacgc cgagcttctt catgtcggct tcgtcgacga tcaccgagcc
     7321 gggctcgtcg agcctcaccc gcatgtcggg tgtcaggacg cgggcgaaca gcagcgcatc
     7381 cggcgaggtg tcgacgcccg agacgtataa cgtgatcgca cccacgtccg gacgcgaacg
     7441 ccagtcacct tcgatccagc tgtaggcctc gacacggtcg accgccgggt cgagccgcag
     7501 ccagatttcg gtgtcccgcg ggatggcacg tccgagctcg acggttcgcg tgcctgggtg
     7561 gcccacccac aggtcgccgc gcgacgcgtc gatgtagacc gccgagctgc cgaagatgcc
     7621 cagcaccagc gcggcctgca ccagcagcag cagccccgag aacgcgactg cgaccacagc
     7681 aggaagaaag cgccgccatt catgcagcag cgacttgcgc gccagtggaa cgatcatggc
     7741 gaaggcgtgg gcaggctggc gccaccgaaa gccgtgaaga gcgcgacata cgcgacctgt
     7801 tcagccaggc cggcccgcag ggtgtcgcgg cgcgcctgaa cggccgcact gcgcgcatcg
     7861 gcacgctgga acccggtagc gaggccgagc cgcgcctgcg cttcggtcgc attcgagcgc
     7921 tggcgcacgg cctcctcgac cgcgaccgcc gcgacggtgc gatgtcgctc gtggtcgagc
     7981 tgcagcagcg ccgactgggc ctcggcaacg ccttgcagca ccgcctgccg atactgggca
     8041 gtggccgcat cgaaatcggc gtcgcgcgca cgctgcgagg cggcgatcgc gccaccatcg
     8101 aacagggaca tcgagaagct cgggccggcg gacagcaccg cgcgcaccgc gccggatgcg
     8161 ggaccggtga tcgcagcgct cagcccgacg agtccggcca ggctgatgcg cggttgcgcg
     8221 tcggcgcggg ccagcccggc ggcatgcgcg gctctcaaca ccgtgtgctc cgcccggcgg
     8281 atgcccggcc gctgccgcac caggtcggcg ggcaacgcgg tcaggcccag cggtgcgcgc
     8341 gcaggaagcg cggaatcgac agctggcggc ggcaaatccg cctgaagatc gcccacgagc
     8401 acaccgagtc gggttcgagc cagagcggcc agcgcaaccg gctcaagggt ggcagcccgg
     8461 gcctgcaccg cagccaccac cgcagcctgg acttcatcgg cgccgacctg attggcctgc
     8521 agcagcacgc gggtgaggcg ctcgcgctcg tcggcctccg cggcgagctg cttcaacagg
     8581 tgttgctcgc gttcggccgc acgcatctcg aagtacgcac gagccacttc ggccaccagc
     8641 gtggcccggc tggcctgctc gtctgccagc gccagttgct cgtcggcttc ggccaatccg
     8701 cgggttgcga tctgccgatc ctgcagggga acgtcccatc gagccgccac gccggcctgg
     8761 aaataggtgc tgcgtccgtt ggcggtctgc tgcggatcgc ccacgagcga gacctgaggc
     8821 cccaggcggg cggccatttc ctgccccatc gcctgtgccg agcgcacccg ggcggcggcc
     8881 gcctcgagat ccggcgaacc cgccagcgcc cgggcgacca gcccgtcaag cgcgctgtcg
     8941 ccgaacgcac gccaccagct tgtcaagtcg accgcatctc ccagcagcgg gggcgcagcc
     9001 cacgccacag gcaacggcac cacgggaacg gcggccggtg gggccggcgc aggggtggac
     9061 gcgcagccgg ccagacaagc caccgcgatg ccgaggatcc ggatacgcag ggcactgggc
     9121 acgcagggga agacagacat cggcatggcc gagatcatag gtgcgccgtc gaaagagcct
     9181 gaaaaacgcc acctgagatc gggatcctgt aggaatttgc ctgacacgat gcatggcaaa
     9241 agagggactc aagcgacaga gatgacctga tgtcagcccc catctctggt caacacaatc
     9301 tgtttcacgt tgcaacgaat ccaaagccga atccggcaac gcgaaaagaa caccgctcaa
     9361 accgatcgac accgcagatc acgaccagga acaccaccat gaatgcagat cagatcctca
     9421 ccgaaatccg cgaagccaac ctgtcctacc tgatgctggc tcagagcctc atccgcacgg
     9481 accgtgaaca ggccctgtat cgcctgggca tcagcgagga aaacgcagcg ctgctgcaga
     9541 tgctgacccc ggcgcagatg atgaagatcg cctcgggcaa cacgctgctg tgccgcttcc
     9601 gcatggacga cgacatggtc tgg
//
LOCUS       DAOORV010000128         9579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8853_length_9579_cov_9.668732, whole genome shotgun sequence.
ACCESSION   DAOORV010000128 DAOORV010000000
VERSION     DAOORV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_9579_cov_9.668732"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKV56_14300"
     CDS             complement(<1..462)
                     /locus_tag="PKV56_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HOX69032.1"
                     /translation="MSERPALCARAIIDRSGAANLIVLLRVRMGFPAESFALAARPLI
                     DGYAEFVQLRPMPGSSRYGGPGGQLQRGLVTALRALDHRRGQILPRNAPPEILGALAH
                     RWTYAVFAAALLRDVGGGPSDERLRMFERWVTPTVQAWLAEHPPLMAELRAV"
     gene            824..1348
                     /locus_tag="PKV56_14305"
     CDS             824..1348
                     /locus_tag="PKV56_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOX69033.1"
                     /translation="MRKTASWAPTAALITAIVTLAALPAHACWDEAAARYRVSSELLY
                     AIARTESALDPQAVGRNRNGTRDIGLMQINSAWLPTLAAHGIGERDLFDPCTSIHVGA
                     WILAGNVQRLGYTWDAVGAYNAANPALRRAYVDKVRRHLRGADDGAHRARHGATRTSI
                     TRGDHRAPVVATLP"
     gene            1397..1693
                     /gene="traA"
                     /locus_tag="PKV56_14310"
     CDS             1397..1693
                     /gene="traA"
                     /locus_tag="PKV56_14310"
                     /inference="COORDINATES: protein motif:HMM:NF041281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HOX69034.1"
                     /translation="MTLIRRSSTAVPIALVLTLAAGSALAGTTGTEFQTMYTTLLNWA
                     TGFLGKSIAIAAFILGAGIGIARSSPIPALAGVVFALFMVYVPTIIDSIMTAVI"
     gene            1706..1996
                     /gene="traL"
                     /locus_tag="PKV56_14315"
     CDS             1706..1996
                     /gene="traL"
                     /locus_tag="PKV56_14315"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HOX69035.1"
                     /translation="MIDASLDIPRRLNDPPRMFWWDIDVALLVLGAALAGMVAGFFVS
                     GCAVGLLLASAYGRAKAGKHPAFALHLLYWHLPAFMTGLKRTPPSYLRELAG"
     gene            1993..2616
                     /gene="traE"
                     /locus_tag="PKV56_14320"
     CDS             1993..2616
                     /gene="traE"
                     /locus_tag="PKV56_14320"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HOX69036.1"
                     /translation="MKLDWLRADIASARRASALLVLLLACSMLANVTLAAFAMHMTGR
                     ERVVVVPPSINKTFWVESERVSAEYLEQMAYFLLQLTLNVTPQSIDHQSRVLLQYAAP
                     ASYGELRSVLATAAERVKRDGASTVFSAQDLAVDERTQRVGVRGLLTTFISDRRVSEV
                     SKGYAIELQYAGGRIFLKAFRETSPNDPLEIQAQSQLRLAPAAGAGR"
     gene            2549..3277
                     /locus_tag="PKV56_14325"
     CDS             2549..3277
                     /locus_tag="PKV56_14325"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HOX69037.1"
                     /translation="MTRSKSKLSPSFAWLLLLALAASQPALALQVVDARDGETVLAKV
                     SRKEVTRISVDRGRIRKVTGNAGEFVLEKDDEKGQVFIRPVSPDSTKPINLFVSTERS
                     TIGLLLQPVDTPSDAIVIREARDAPTGPARIERSGRHVRTMKNLLLAMAEDALPDDME
                     VREPGRALTLWPGARLTLQRQWLGSGVVGEKYQLVNTGSSALDLAERDLFKRGVMAVS
                     VEQAALRPGETTQLFVIRERRADD"
     gene            3270..4670
                     /locus_tag="PKV56_14330"
     CDS             3270..4670
                     /locus_tag="PKV56_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HOX69038.1"
                     /translation="MTDPVLPPGMSPTGASHPSGQGSPSAPTEPTAAAIKRRQVLLLA
                     GIAGTIVAGTLLSVSLTGTKGNDTQPAKPQSTNILAPGAQVDPRDAWRGQADAQLKAI
                     EQRSRDLAQRNAELEGQGKEMLERLKKLEGSGLTPLPPPPVTAPAARPSFGPDRPGSA
                     LPDAGPQRFPPPPPMPQGAVQGAGLPPPPGGVSSTPTPTGIVSIVLGDVAAGKGAKPA
                     ADAAAAAPASRDTRRYLPSGAFTRAVLLGGLDAPTGGQAQRNPQPVLLRLADNAILPN
                     QFRARVKECFIVGAGYGDVSSERAYIRTESLSCVTRDGTAIDVPVKGYVAGEDGKAGM
                     RGRLVSKQGQILANALLAGVASGIGHAFTQSSTTLSVSPLGTTSTVDPGKQLEAGLGT
                     GVGKALDRLAQYYISLAEKVFPVIEVDAGRSVDVVLTQGVALPGTLDAAGTDPDNLAQ
                     LAERAHTLRRNDDEDD"
     gene            4657..5487
                     /locus_tag="PKV56_14335"
     CDS             4657..5487
                     /locus_tag="PKV56_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOX69039.1"
                     /translation="MKTTDARLTLRALAAKATLAAALTTCLSVAAWAQPSQPPTTPEL
                     NSLIGRLQALYPSTRFGEIRPTPWPGVFEVAMGANLAYVDASGQYFLFGHLYDMKAQR
                     DLTAERKDTLARIDFNALPLADAIKEVRGRGSRALAIFSDPDCPHCRRLEAELKGLSD
                     VTIHTFLMPIASLHPQARAKAIAVWCAKDRLGAWQALMTRDQVPSSADCAHPVDRNVA
                     LADRLGVTGTPTLVAADGRVLPGAASAEQINAWLSRSTTSAEGPAPGIPAAQATGKAP
                     "
     gene            5559..6074
                     /gene="traV"
                     /locus_tag="PKV56_14340"
     CDS             5559..6074
                     /gene="traV"
                     /locus_tag="PKV56_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HOX69040.1"
                     /translation="MSGLGGEGSYACKAPVGSQCTSVSGVYANSIHGQPPASALPKPA
                     NEPTSTAAAATVASASTAAPGLGAPPSALRSQPRVLRLWIAPWEDADGDLHEASVVHV
                     LVDTGRWLIERVIPANRQRVDAVRPPIPSAAPASGPASASEPASSTETAPDRFPPRTG
                     LLPGNGATQEP"
     gene            6078..8645
                     /gene="traC"
                     /locus_tag="PKV56_14345"
     CDS             6078..8645
                     /gene="traC"
                     /locus_tag="PKV56_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HOX69041.1"
                     /translation="MWRSLLTDLESVLKGAKAADLIRVPKSVPASRGEDAAAFSEWLP
                     YRAWIADRQVFVNLDALGFCLEVRPQSGADEEMARVLTALYAASPPGTGIQFHLYASP
                     AIRAPLARYANLRVADDVVPELDTLGRAGRHTNIHRTMARRRAGHYLQGSRTSLLSAQ
                     SYLFRDYRLVVSVSLPGSPENLSRLEELLLLRDGIRATLHAAGFPSRSWTAEDLINWV
                     SALLDPHRQTGDAIPLTYDDGRELRDQVIDRATRLRIDRQGIGLANPAADLQTELRLM
                     SVRAFPPRFALWNAGSLIGDLYQGTLQYPCPFLLTLGVHVLDAEATRNWAFLKAARAT
                     TNATSYMARFLPDLQERKADWDIVLKAMDDGQQLVNLNLQLALFAEPNTVTRAEQAAR
                     AIFRARGFELSSDTMMMTQALIGSLPMTLSAPFHADLQRMKRVTTKTSANAVHLAPLV
                     AEWQGTGTPVLLFGGRRGQIMQIDVYDNPAGNYNVAIAGTSGSGKSLLLNEIAAAYLG
                     TGARVWIIDVGRSYEKACRNFGGSFIEFTEDAALSLNPFPLVEDIDEDMELLQPLLAQ
                     MVSPRESLDGFQYSTLGAAIKKVWKAKGRAMTVTDIHDLLATGRLDDEGAPSAGEGDR
                     RLKDLAAMLHPYTRDGAYGKYFDSEASIDFGADLIVLELEELKSKKDLQTVVLLIVMY
                     RITREMYFSRDRKKIVIIDEAWDLLSGGATAEFIEAGYRRARKYKGAFMSATQGVDDY
                     YRNPAAKAALDNSDWMFLLRQKPESIEMMDKLGQLTMDDAMKRLLLSLRTEHGAYSEV
                     FIHSPAGNGIGRLIVDPYSLLLFSSRAEDFNAINAKRAAGLDVSAAIDAVLRERGLA"
     gene            8642..9007
                     /locus_tag="PKV56_14350"
     CDS             8642..9007
                     /locus_tag="PKV56_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69042.1"
                     /translation="MNARAAALLVGVNALVSAALIALVHLWLVPERLPALAVLDVAEL
                     YRLKELQVAAVLVKRDASDEERAGALRRAAGFGTEVSTLLQALPSECRCIVLARGAVM
                     GSEPLLRDLTPDVRRRLGL"
     gene            9025..9579
                     /locus_tag="PKV56_14355"
     CDS             9025..9579
                     /locus_tag="PKV56_14355"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOX69043.1"
                     /translation="MRTLFLNRSWRDRFASSGRFRLRKFAERSVEHLKHWAVVYVAVA
                     AIALWFHAHYGFGLNASPSLPHRLFLIHKGEMPSRGDFVAFRWAGGGPYPAGVTFIKV
                     LAGMPGDEVTRDSQGFHLNGIPVGVPKPVSRRGQPLEPGPTGRIPEGRYYVQAGHPDS
                     LDSRYQLTGWIHASQIIGRADALF"
BASE COUNT         1494 a         3591 c         3108 g         1386 t
ORIGIN      
        1 cacggcgcgc agttccgcca tcagcggagg atgttcggcc aaccatgcct ggacggtcgg
       61 tgtaacccac cgctcgaaca ttcgcaaccg ctcgtccgac ggacctccgc cgacatcgcg
      121 cagcagcgct gccgcgaaca ccgcgtacgt ccatcgatgc gcgagcgcgc ccaggatctc
      181 gggcggcgca ttgcgcggca ggatctggcc gcgccggtga tcgagcgcgc gcagcgcagt
      241 cacgagcccg cgctgcagtt gtccgccggg gccgccgtat cgactggagc ccggcatcgg
      301 ccgcagctgc acgaactccg catagccgtc gatcagtggc cgcgccgcca gcgcgaagct
      361 ctccgccgga aaccccatcc tgacgcgaag caggacgatc aggttcgccg ccccgctgcg
      421 atcgatgatc gcccgcgcgc agagcgccgg ccgctcggac acgcatgacg actcgatcac
      481 tcgcggcgac gcagacccgg agggcatgtc gttgaaggtc attcgaccag tattccgggc
      541 gccttgacgg ccatcagctt cgatcgaagc ctcgacgagg acccgatgtc ctcttgacga
      601 gttcgaccga acgccgtcgt gagccacgcc gcagcgtcgg ctcttcgcat cggccgctcg
      661 tcgcacatcg tcggcagaga ttccgcgccg atgcaagccg gattcggggc ttcatcgaag
      721 tcccgatgga gcctgattca cacgtgctcg ctccctagga tggcaacgca atgcggctcg
      781 ctcaatgcgc cgcacatccg cgccaacgtg aagggggttc cgattgcgca agacagcgag
      841 ctgggcaccg acggcagcac tgatcaccgc gatcgtcacg ctcgcggccc tgcccgccca
      901 cgcttgctgg gacgaggcgg ccgcgcgcta ccgcgtcagc agcgagctgc tctacgcgat
      961 cgcgcgcacc gaatcggcac tcgacccgca ggccgttgga cgcaaccgca acggcacgcg
     1021 cgacatcggg ctcatgcaga tcaactcggc gtggctgccc acgctggccg cccacggcat
     1081 cggcgagcga gatctcttcg acccctgcac cagcatccac gtcggcgcct ggatcctcgc
     1141 cggcaacgtc cagcgcctgg gctacacgtg ggatgcggtc ggtgcctaca acgccgccaa
     1201 tcccgccttg cgccgcgcct acgtcgacaa ggtccgccgc cacctgcgcg gcgccgacga
     1261 cggcgcacac cgcgctcgcc acggcgccac ccgcacatca atcacacgcg gcgaccaccg
     1321 agcaccggtg gtcgcaaccc tgccctgacc gctcgcccga gcacttcctc ttcacccaac
     1381 cacgagacac cacaccatga ctctgatccg ccgcagcagc actgccgttc ccatcgccct
     1441 cgtcctgacg ctcgccgccg gctcggccct ggccgggacc accgggaccg agttccagac
     1501 gatgtacacg accctgctca actgggcgac cggcttcctg ggcaagtcga tcgcgattgc
     1561 cgccttcatc ctcggtgcag gcatcggcat cgcgcgctcc tcgccgatcc cggcgctcgc
     1621 cggcgtcgtc ttcgcgctct tcatggtcta cgtgccgacc atcatcgact cgatcatgac
     1681 cgccgtgatc tgacaggacg atgccgtgat cgacgcgtcc ctcgacatcc cccgtcgcct
     1741 caatgacccg ccgcgcatgt tctggtggga catcgacgtg gccctgctgg tgctgggggc
     1801 cgcgctggcg ggcatggtcg cgggcttctt cgtgagcggc tgcgccgtcg gcctgctgct
     1861 cgcctcggcc tacggtcgcg cgaaggcggg caagcacccc gccttcgcgc tgcacctgct
     1921 gtactggcac ctgccggcct tcatgacggg cctgaagcgc accccgcctt cctacctgcg
     1981 cgagctggcc ggatgaagct cgactggctg cgcgccgaca tcgccagcgc gcgccgcgcg
     2041 agcgccctgc tcgtgctgct gctcgcctgc tcgatgctcg ccaacgtgac gctagccgcc
     2101 ttcgcgatgc acatgaccgg ccgcgagcgt gttgttgtgg tgccgcccag catcaacaag
     2161 accttctggg tcgagtccga gcgcgtcagc gccgagtacc tcgaacagat ggcgtatttc
     2221 ctcctgcagc tgacgctgaa cgtcacgccg cagagcatcg accaccagtc ccgggtgctg
     2281 ctccagtacg ccgcgccggc ctcctacgga gagctgcgca gcgtgctggc caccgccgcc
     2341 gagcgcgtca agcgcgacgg cgcctccacc gtgttcagcg cgcaggacct cgccgtcgac
     2401 gagcgcaccc agcgcgtggg cgtgcgcggc ctgctcacca cattcatcag cgaccgccgc
     2461 gtgtccgagg tctcgaaggg ctacgccatc gagctgcagt acgccggcgg ccggatcttt
     2521 ctgaaagcgt tccgggagac cagtcccaat gacccgctcg aaatccaagc tcagtcccag
     2581 cttcgcctgg ctcctgctgc tggcgctggc cgctagccag cccgcgctag cgctgcaggt
     2641 cgtcgatgct cgcgacggcg agaccgtgct cgccaaggtc tctcgcaagg aggtgacgcg
     2701 tatctccgtc gatcgtggcc gcatccgcaa ggtgacgggc aacgccggcg aattcgtgct
     2761 ggagaaggac gacgagaagg gccaggtctt cattcggccg gtctccccgg acagcaccaa
     2821 gcccatcaac ctcttcgtca gcacggagcg ctcgacgatc gggctgctgc tgcagccggt
     2881 ggacaccccg agcgacgcca tcgtgatccg cgaggcccgc gacgccccga ccggacccgc
     2941 acgcatcgag cgcagcggcc gtcacgtgcg cacgatgaag aacctcctgc tcgcgatggc
     3001 cgaggacgcg ctgccagacg acatggaggt gcgcgagccc gggcgcgcgc tgaccctgtg
     3061 gcccggcgcg agactcacgt tgcagcgcca gtggctgggc agcggcgtgg tcggcgagaa
     3121 gtaccagctc gtcaacaccg gctcgtccgc gctcgacctc gccgagcgcg atcttttcaa
     3181 gcgcggcgtg atggccgtga gcgtcgagca ggcggccctg cgcccgggcg agaccaccca
     3241 gctgttcgtg atccgggagc gccgcgccga tgactgatcc cgtcctgccg cccggcatgt
     3301 cgccgaccgg cgcatctcac ccgtcggggc aagggtcccc gtcggccccg acggagccaa
     3361 cggccgcggc catcaagcgc cgccaggtcc tgctgctcgc cggcatcgcc ggcacgatcg
     3421 tggccggcac gctgctgtcg gtgtcgttga ccggaacgaa aggcaacgac acgcagcccg
     3481 cgaagccgca gtccaccaac atcctcgcgc cgggtgcgca ggttgatccg cgcgacgcgt
     3541 ggcgtggcca ggccgatgcg cagctgaagg cgatcgagca gcgctcgcgc gacctcgcgc
     3601 agcgcaatgc cgagctcgaa ggccagggca aggaaatgct cgagcggctg aagaagctcg
     3661 aaggcagcgg actgacgcca ctgccgcctc cgcccgtcac cgccccggcg gcacggccga
     3721 gtttcgggcc ggatcgcccg gggagtgccc tcccggacgc cggtccgcag cgattcccgc
     3781 cgccgccacc catgccgcaa ggcgccgtgc agggcgccgg cctgccaccg ccgcccggcg
     3841 gggtttcgag cacgccgacg cccacgggca tcgtcagcat cgtgctgggg gacgtcgcag
     3901 cgggcaaggg cgccaagccg gccgcggatg cggcggccgc cgcaccagcg agtcgagaca
     3961 cgcgccgcta tctgcccagc ggcgccttca cccgggcggt acttctcggc ggcctggacg
     4021 cacccaccgg cggccaggcc cagcgcaatc ctcaaccggt cctgcttcga ctcgcggaca
     4081 acgcgatcct gcccaaccag ttccgcgcgc gcgtgaagga gtgcttcatc gtcggcgccg
     4141 gctatggcga cgtgagttcc gagcgcgcct acatccgcac cgagtcgctg tcgtgcgtca
     4201 cccgcgacgg cacggccatc gacgtgccgg tgaagggcta tgtggcgggc gaggacggca
     4261 aggccggcat gcgcggacgc ctggtgtcca agcaggggca gatcctcgcc aacgcgctgc
     4321 tcgccggcgt ggccagcggc attggtcacg ccttcacgca gagctccacc acgctgtcgg
     4381 tctcgccact gggcaccacc agcaccgtcg accccggcaa gcagctcgaa gccggtctgg
     4441 gcaccggcgt gggcaaggcc ctggaccggc tcgcccagta ctacatcagc ctcgccgaaa
     4501 aagtcttccc ggtgatcgag gtcgacgcgg gccgcagcgt cgacgtggtc ctcacgcagg
     4561 gcgtcgccct gcccgggacg ctggacgccg ccggcaccga tcccgacaac ctcgcgcaac
     4621 tcgccgagcg cgcccacacc ctccggagga acgacgatga agacgactga cgcgcgcctc
     4681 acgctgcgcg ctctcgctgc caaggccacg ttggccgcgg cgctgaccac ctgcctgagc
     4741 gtcgcggcat gggcgcagcc gagccaaccg ccaaccacgc ccgaactgaa cagcctcatc
     4801 gggcgcctgc aggcgctcta cccctcgacc cgcttcggcg agatccgtcc gacgccgtgg
     4861 ccgggtgtct tcgaagtggc gatgggcgcc aacctcgcct acgtggacgc gagcggccag
     4921 tacttcctgt tcggccacct gtacgacatg aaggcacagc gcgacctcac cgccgagcgc
     4981 aaggacacgc tggcgcgcat cgacttcaac gcgctgccgc tggccgacgc gatcaaggag
     5041 gtgcgcggcc gcggttcccg tgcgcttgcg atcttcagcg atccggactg cccgcactgc
     5101 cgccggctgg aagccgagtt gaagggcctg tccgacgtga cgatccacac cttcctgatg
     5161 ccgatcgcct ccctgcatcc gcaggcgcgc gcgaaggcca tcgccgtgtg gtgcgcgaag
     5221 gatcgcctcg gcgcctggca ggcgctgatg acccgcgacc aggtgccgtc cagcgcggac
     5281 tgcgcacacc cggtcgatcg caacgtcgca ctggccgacc gcctcggcgt gaccggcacc
     5341 ccgacgctgg tcgccgccga cggtcgcgtg ctgccaggcg ccgcgagcgc cgagcagatc
     5401 aacgcctggc tgtcgcgctc gactacgagc gccgaggggc ccgcgccggg catccccgcg
     5461 gcccaggcga cgggcaaggc gccatgacgg acctgcgacg gaccgtcgcc ctcctcgtcg
     5521 gcgcgaccgc cacgctgttg ggaggctgcg cgtccaccat gagcggcctc ggcggcgagg
     5581 gcagctacgc ctgcaaggcg ccggtcggtt cgcagtgcac ctcggtctcg ggcgtgtacg
     5641 cgaactcgat ccacggccag ccgcccgcct ccgcgctgcc gaagcccgcg aatgagccca
     5701 cgagcacggc agccgccgcg acggtcgcct cggcatccac agccgctccg gggcttggcg
     5761 caccgccatc ggcactccgc tcccagccac gcgtgttgcg cctgtggatc gcgccctggg
     5821 aggacgccga cggcgacctg cacgaggcct cggtggtgca cgtgctggtc gacaccggcc
     5881 gctggctgat cgaacgcgtc attccggcga atcgacagcg cgtcgatgcc gtgcggcctc
     5941 cgattccgtc cgctgcgccc gcctccggcc cggcatcggc gagcgagccc gcgtcgtcca
     6001 cagagacagc gcccgatcgc tttcccccgc gcacgggact gctgcccggc aatggcgcga
     6061 cccaggagcc ctgagcgatg tggcgcagcc tgctgaccga tctggagtcc gtgctgaagg
     6121 gcgcgaaggc cgcggacctg atccgcgtgc ccaagtccgt ccccgcatcg cgcggcgagg
     6181 acgccgccgc gttctccgag tggctgccgt accgcgcctg gatcgcggac cgccaggtct
     6241 tcgtcaatct cgacgcgctg ggcttctgcc tggaagtgcg accgcaatcc ggcgccgacg
     6301 aggagatggc gcgcgtgctg accgcgctct acgcggcctc tccgcccggc acgggcatcc
     6361 agttccacct gtacgcgagc cccgcgattc gcgcaccgct ggcgcgctac gcgaacctgc
     6421 gtgtcgccga cgacgtcgtc ccggagctcg acaccctcgg ccgcgccggc aggcacacca
     6481 acatccaccg caccatggcc cggcgtcgtg ccgggcacta cctgcaaggc agccggacgt
     6541 cgctgctgtc cgcgcagagc tacctcttcc gcgactaccg gttggtggtc agcgtctcgc
     6601 tacccggcag cccggagaat ctctcgcgcc tggaggagtt gctgctgctg cgcgacggga
     6661 tccgggccac gctacacgcc gcgggctttc cgtcccgttc gtggacggcg gaggacctga
     6721 tcaactgggt ctccgcgctg ctcgacccgc accggcaaac cggcgacgcc atcccgctca
     6781 cctacgacga cgggcgcgag ctgcgcgacc aggtgatcga ccgggccacg cgcctgcgca
     6841 ttgaccgtca aggcatcggc ctggccaacc cggccgccga cctgcaaacc gagcttcgcc
     6901 tgatgtcggt gcgcgccttc ccgccgcgct tcgcgctgtg gaacgccggc agcctgatcg
     6961 gcgacctcta ccagggcacg ctgcagtacc cctgcccgtt cctgctcacg ctgggcgtgc
     7021 acgtgctcga cgccgaggcc acccgcaact gggccttcct caaggccgcc cgcgccacca
     7081 cgaacgccac cagctacatg gcgcgcttcc tgcccgacct gcaggagcgc aaggccgact
     7141 gggacatcgt gctgaaggcg atggacgacg gccagcagct cgtcaacctc aacctgcagc
     7201 tcgcgctctt cgccgagccg aacacagtga cccgtgccga acaggcggcg cgcgccatct
     7261 tccgtgcgcg tgggttcgaa ctctccagcg acacgatgat gatgacccag gccctcatcg
     7321 gctcgctgcc gatgacgctc tcggcgccgt tccacgccga tctgcagcgc atgaagcgcg
     7381 tcaccaccaa gacctcggcg aacgcggtgc acctggcgcc gctcgtcgcc gagtggcagg
     7441 gcacgggcac gcccgtgctt ctgttcggcg ggcggcgcgg ccagatcatg cagatcgacg
     7501 tgtacgacaa cccggcgggc aactacaacg tggcgatcgc cggcacgtcg ggctcgggca
     7561 agtcgctgct gctgaacgag atcgccgccg cctacctcgg caccggcgcg cgcgtctgga
     7621 tcatcgacgt cggccgctcc tacgagaagg cgtgccgcaa cttcggcggc agcttcatcg
     7681 agttcaccga ggacgccgcg ctgtcgctca acccgttccc cctggtcgag gacatcgacg
     7741 aggacatgga actcctgcag cccctgctcg cgcagatggt gtccccgcgc gagtcgctcg
     7801 acggctttca gtactcgacg ctaggtgcgg cgatcaagaa ggtctggaag gccaagggcc
     7861 gcgccatgac cgtcaccgac atccacgacc tgctcgcgac cggacgcctc gacgacgaag
     7921 gcgcgccgtc cgccggcgaa ggcgaccggc ggttgaagga cctcgccgcg atgctgcacc
     7981 cctacacgcg cgacggcgcc tacgggaagt acttcgattc cgaggccagc atcgacttcg
     8041 gcgccgacct catcgtgctg gagctcgagg agctcaagtc gaagaaggat ctgcagacgg
     8101 tggtgctgct gatcgtcatg taccgcatca cgcgcgagat gtacttctct cgcgaccgca
     8161 agaagatcgt gatcatcgac gaggcctggg acctgctctc cggcggcgcc accgccgagt
     8221 tcatcgaggc cggctaccgc cgagcgcgca agtacaaggg cgccttcatg tcggccaccc
     8281 agggcgtgga cgactactac cgcaacccgg cggcgaaggc ggcactggac aactccgact
     8341 ggatgttcct gctgcgccag aagcccgagt cgatcgagat gatggacaag ctcggtcaac
     8401 tcacgatgga cgatgcgatg aagcggctgc tgctgtcgct gcgcaccgag cacggcgcct
     8461 actccgaggt cttcatccac tcgccggccg gcaacggcat cggccggctg atcgtcgatc
     8521 cgtacagcct cctgctcttc agcagccggg ccgaggactt caacgccatc aacgccaagc
     8581 gtgcggcggg cctggacgtc tccgccgcca tcgacgcggt cttgcgcgaa cggggtctcg
     8641 cgtgaacgca cgggccgccg cactgctcgt gggcgtcaac gcgctggtga gcgccgcgct
     8701 gatcgcactc gttcacctgt ggctcgtgcc cgagcgcctg ccggcgctgg ccgtgctgga
     8761 cgtggcggag ctctatcgcc tgaaggagct gcaggtcgcc gcggtgctgg tcaagcgcga
     8821 cgccagcgac gaagagcgcg ccggcgcact gcgtcgcgcc gcgggcttcg gcacggaggt
     8881 gagcacgctg ctccaggcgc tgccgagcga gtgccgctgc atcgtgctgg cgcgtggcgc
     8941 cgtgatgggc tccgaacccc tgctgcgaga cctgacgccg gacgtgcggc gccggctcgg
     9001 cctgtagctg cattgggagc ccgcatgcgc accctgttcc tgaatcgaag ctggcgcgac
     9061 cgtttcgcat cgagcggacg attccggctg cgcaagttcg ccgagcgctc ggtcgaacac
     9121 ctgaagcact gggccgtcgt ctacgtcgcg gtcgccgcga tcgcgctctg gtttcacgcc
     9181 cactatggct tcgggctgaa cgcctcgccg agcctgccgc accggctctt cctcatccac
     9241 aagggcgaga tgcccagccg aggcgacttc gtcgccttcc gctgggccgg cggcggaccg
     9301 tacccggcgg gcgtcacctt catcaaggtg cttgccggca tgcccggcga cgaggtcacg
     9361 cgcgattcgc agggcttcca cctcaacggc attccggtgg gcgtgcccaa accggtgagc
     9421 cgaagaggtc agccgctcga acccggtccc accggccgca ttccggaggg ccgctactac
     9481 gttcaagccg gacacccgga cagcctcgac tcccggtacc agctgaccgg ctggatccac
     9541 gcgtcccaga tcatcgggcg ggccgatgcg ctcttctga
//
LOCUS       DAOORV010000129         9539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_8896_length_9539_cov_8.229966, whole genome shotgun sequence.
ACCESSION   DAOORV010000129 DAOORV010000000
VERSION     DAOORV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8896_length_9539_cov_8.229966"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3473
                     /locus_tag="PKV56_14360"
     CDS             <1..3473
                     /locus_tag="PKV56_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOX69044.1"
                     /translation="FNAGDGRCPTCGGSGFEHVEMQFLSDVYLRCPDCDGRRFRRELL
                     EVKIDRRLPGEVLRDLSVADVLDLTVSEAVQLFANDREVLRVLQPIVDVGLEYVKLGQ
                     PVPTLSGGEAQRLKLAGFLAESANSATASRQAVAKKGTLFMFDEPTTGLHFDDIAKLM
                     RAFRKLLDAGHSIIVIEHNLDVINASDWLIDLGPEGGEGGGQVVCFGTPADMKRHPTS
                     HTGRALVDYDRAMGLGGLVVEEGVPLQVLLKAARAERVAVDESIRIVNAREHNLKSLD
                     VSIPRGQFTVVTGVSGSGKSTLAFDILFNEGQRRYLESLNAYARSIVQPAGRPEVDAV
                     YGIPPTVAIEQRLSRGGRKSTVATTTEVWHFLRLLYVKLGLQHCVKDGAEVRPQSAES
                     IAAQLLRDHRGEHVGLLAPLVVARKGVYTDLAKWAKARGHTHLRVDGEFVPVEPWPRL
                     DRFKEHTLELPVADILVSADREGELRAALAKALELGKGVMHLLTPLNGLTEAMRSGSS
                     TLGIGRLKVFSTKRACPTCGTSYPELDPRMFSYNSKHGWCTTCVGTGLALTREQRKVY
                     DDSVRDDDSKGREQSFPSEESEAEGLVDEPCPDCAGTRLNPVSGQVTFEGHSIAGIAR
                     WSVADARRWVESLTLTGRDAEIARDVVSEIRGRLEFLEEVGLGYLTLDRAAPTLSGGE
                     AQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNQILLKALKSLSDKGNTLVVVEHDED
                     TIRRADHLIDIGPGAGTRGGRLVAQGTVADLQGAADSQTGRLLAHPLIHPLQARREVP
                     AAEEAGPRESESNRRLTVQGATLHNLQRVTIDVPLKRLVAITGVSGSGKSTLARDVLL
                     ANLQFINVKGGAPKWQGCNSIEGWGYIDRVLEVDQTPIGKTPRSCPATYIGFWDTIRK
                     LFADTLESRARGYAPNRFSFNTGEGRCPACEGQGMRTIEMSFLPDVKVPCDQCHGQRF
                     NPETLAVTWRGRNIGEVLGMEIDEAVDFFASMPAVSHPLRLMKDVGLGYLTLGQPSPT
                     LSGGEAQRLKLVTELTKVRDDVGKRGNKAPHTLYVLDEPTVGLHMADVEKLIRVLHRL
                     VDGGHSVVVIEHDLDVIAEADWVIDLGPEGGTQGGRVVAAATPEDLANRSDSHTGVAL
                     RPVLGRRPGQ"
     gene            complement(3571..5166)
                     /gene="ahpF"
                     /locus_tag="PKV56_14365"
     CDS             complement(3571..5166)
                     /gene="ahpF"
                     /locus_tag="PKV56_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOX69045.1"
                     /translation="MLDDTLKAQLQAYLERVKIPFEIVASLDDSDASREMHGLLEDII
                     SLCDKITLRTDGTDARKPSFTLARIGETPRIRFAAIPLGHEFTSLVLALLQVGGHPAK
                     VEADLIEQIKNLDGDFRFETYVSLSCHNCPDVVQALNLMAVLNPRVQHVTIDGGLYQQ
                     EVEDRQVLAVPMVFLNGEHFGQGRMEAEEIVSKLDKGAAARDAVKISAKAPYDVLIVG
                     GGPAGAAAAVYAARKGIRTGVMAERFGGQTLDTLGIENFISVLETDGPKFAMALEQHA
                     RQYGVDIMNLQRAEKLIPAAQPNGLIEVQLANGGSLKSKTVILSTGARWREVNVPGEK
                     EYRNHGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVGHVTLIEFGEQLRAD
                     AVLVNKLNSLANVTIHTQAQTTEITGDGAKVNGLTYTDRPSGASHHINLEGVFVQIGL
                     VPNSEWLKGTLELSRHGEIVVDARGQTSVPGVFAAGDVTTTPFKQIIIATGEGAKAAL
                     SAFDHLIRTSAPAAATAKQGATV"
     gene            complement(5311..5874)
                     /gene="ahpC"
                     /locus_tag="PKV56_14370"
     CDS             complement(5311..5874)
                     /gene="ahpC"
                     /locus_tag="PKV56_14370"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOX69046.1"
                     /translation="MSLINTQIKPFSATAYQNGKFIDITQDTLKGKWSAIVFYPADFT
                     FVCPTELEDLADNYAEFQKIGVEVYGVSTDTHFTHKGWADASPAIKKVKYPLVGDPTG
                     TLSRNFEVMIEEAGLALRGTFVINPAGQIKVLEVLDLGIGRDAKELLRKIKAAQYVES
                     HPGEVCPAKWSEGAKTLTPSLDLVGKI"
     gene            complement(6012..6968)
                     /locus_tag="PKV56_14375"
     CDS             complement(6012..6968)
                     /locus_tag="PKV56_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX69047.1"
                     /translation="MQPAQNETLPPLAPVVAVLLCSYQGERHLAEQLDSIAAQTHRGW
                     TLWVSDDRSTDGTVAILEQYRSRWGSDRLSILTGPARGFAANFRSLTCHAEIDADGYA
                     WCDQDDVWEPEKLERALAWLQSVPAGTPALYGSRTLLTDEENRPIGMSPIFTRPPCFR
                     NALVQSIAGGNTMVFNRATRDLLIQANREGDAVAPDWLAYLLVSGCGGQVRYDPWPSV
                     RYRQHDENLSGSNVSLRGRWQRMRMLLGGQFKGWVDANVGLLERVRSRLTEENLRILD
                     TFVQARREPMPARLLGLRRAGVHRQTAVGTIGLAVAALLNRL"
     gene            7080..>9539
                     /locus_tag="PKV56_14380"
     CDS             7080..>9539
                     /locus_tag="PKV56_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX69048.1"
                     /translation="MTSNDVPLHVYRREISTSEQTSLSVIAGLIQPGCSVLDLGTGSG
                     ALGRHLREQQACVVDGVTLNDAEAELARPHYRRIEVADLEQAPLTELFQGAAYDCIVC
                     ADVLEHLKQPEQVLSACRELLAPTGKVLISVPNAGYCGLIAELLEGEFEYRPEGLLDS
                     THLRFFTRRSLGRFLASEGWSMVSLLPVLRELGASEFRTRFDSLPPAVGRYLLAVPDA
                     LAYQFVAEVRPATSASLAVPVDSEPTQPAAQALFTAELFLGQSGAYSQQHKQVVAGVI
                     GRSRQTLRFAWGDDVPAWTSIKLDPADRPGFIHLYRISLMSSNGECVWEWRCERDSVS
                     SLEASASQQLLLRPPWPEGAPALALLHGDDPWIELPIAPAALAAMGSRHGGALEVEVG
                     WPMSADYLALKDVVKTVDDRLKSVQKHLDQQVEERDHLAREVVSLAQVESRLSAQRDE
                     LLKQKGLLVDEQTELRNQHFKLRDDYAELAAHLQDIERSRLFRMTRPLSHLKGWVDRN
                     LRGRPGRPNAVVQATALVSTPVTPTPCPVDVIVPVYRGLADTQLCLESVLASRNDSAF
                     RLIVINDASPEPELAGWLRELRDRDQRVVLVENEQNLGFVGTVNRGMAMSDAHDVILL
                     NSDTEVANDWLDRLKRAAYSDARVGTVTPFSNNATICSYPRFCESNTLPAGLSTAALD
                     ALFARTLAGQTIDVPTGNGFCMYVRRDCLAQVGLFDVEQFGKGYGEENDFCQRAQEAG
                     WRNLHALDTFVLHSGGVSFGASKGAREIAAMETLRRLHPAYEQQVHRFIALDPALPAR
                     QAVDLARVSAAGKPVVLLVMHD"
BASE COUNT         1575 a         3127 c         3188 g         1649 t
ORIGIN      
        1 gcttcaacgc cggcgacggg cgctgcccga cctgcggcgg ctcgggcttc gagcacgtcg
       61 agatgcagtt tctcagcgac gtctacctgc gctgcccgga ctgcgatggc cggcgctttc
      121 gccgggaact tctcgaagtc aagatcgatc gacgcctgcc gggagaggtc cttcgcgacc
      181 tctcggtggc ggatgtgctc gatttgacgg tcagcgaagc ggtgcagctg ttcgccaacg
      241 accgcgaggt gctgcgcgtg ctgcagccca tcgtcgatgt cggactcgag tacgtgaagc
      301 tcggccagcc ggtgccgacg ttgagcggcg gcgaggccca gcggctcaag ctcgccgggt
      361 tcctcgccga gtcggcgaac agcgcgaccg cgagccgcca ggcggtggcg aagaagggca
      421 cgctcttcat gttcgacgag ccgaccaccg gcctgcattt cgatgacatc gcgaagctga
      481 tgcgcgcgtt ccgcaagctg ctcgacgccg gacattcgat catcgtcatc gagcacaacc
      541 tcgacgtgat caacgcgtcc gactggctga tcgatctcgg ccccgaaggc ggtgagggcg
      601 gtggccaggt cgtgtgcttc ggcacccccg ccgacatgaa gcggcacccg acctcgcaca
      661 ccgggcgcgc gctggtcgac tacgaccgcg ccatggggct gggcggtctg gtggtcgagg
      721 aaggcgtgcc gctccaggtg ttgctgaagg cggcgcgcgc ggagcgggtc gctgtcgacg
      781 aatcgatccg catcgtcaat gcccgcgagc acaacctgaa gtcgctggac gtcagcatcc
      841 cgcgcggcca attcaccgtg gtcaccggcg tgtcaggttc gggcaagagc acgctggcct
      901 tcgacatcct tttcaacgag ggccagcgcc gctacctcga aagcctgaac gcctacgcgc
      961 gcagcatcgt gcagccggcg gggcgacccg aagtcgacgc ggtctacggg atcccgccga
     1021 cggtcgccat cgagcagcgc ctgagccgcg gcggccgcaa gagcacggtc gcgacaacca
     1081 ccgaggtgtg gcatttcctg cggttgctct acgtcaagct cggcttgcag cattgcgtga
     1141 aggacggggc cgaagtgcgc ccgcagtctg ccgagagcat cgccgcgcaa ttgctgcgag
     1201 accaccgcgg cgagcacgtc ggcctgctgg cgccgctggt ggtggcgcgc aagggcgtct
     1261 acaccgacct cgccaaatgg gccaaggcgc gcggccacac gcatctgcgc gtcgatggcg
     1321 agttcgtgcc ggtcgagccg tggccgcggc tggatcgctt caaggaacac acgctggagt
     1381 tgccggtcgc cgacatcctc gtcagcgccg accgcgaagg cgaactgcgc gccgcgctcg
     1441 cgaaggcgct cgagctcggc aagggcgtga tgcacctgct gacaccgctg aacggcctga
     1501 ccgaagcgat gcgcagcggg tcgtcgacgc tgggcatcgg ccgattgaag gtgttctcga
     1561 cgaagcgagc ctgcccgacc tgcggcacca gctaccccga gctcgacccg cggatgttca
     1621 gctacaacag caagcacggc tggtgcacga cctgtgtcgg cacggggctg gcgctgacgc
     1681 gcgagcagcg caaggtctac gacgattcgg tgcgcgacga tgacagcaag ggccgcgagc
     1741 agagcttccc gtccgaggaa agcgaagccg aaggcctggt cgacgagccc tgtcctgatt
     1801 gcgccggcac gcggttgaat ccggtgtctg ggcaggtcac cttcgaggga cattcgatcg
     1861 cggggatcgc gcgatggtcg gtggccgatg cccgccgatg ggtcgagtcg ctgacactca
     1921 ccggccgcga cgcggagatc gcccgcgatg tggtcagcga gatccgcggc cggctcgagt
     1981 tcctcgagga agtcggcctc ggctacctga cactcgaccg cgccgcgccg acgctcagcg
     2041 gcggcgaggc acagcgcatc cggcttgccg cccagctcgg cagcaacctg cagggcgtct
     2101 gctatgtgct cgacgaaccg acgatcgggc tgcacccgcg cgacaaccag atcctgctga
     2161 aggcgctgaa gtcgctcagc gacaagggca acacgctggt ggtggtcgag cacgacgagg
     2221 acacgatccg ccgcgccgat cacctgatcg acatcggacc cggcgccggc acccgcgggg
     2281 gccgcctggt cgcccagggc acggtcgccg acctgcaagg cgcagccgac tcgcagaccg
     2341 gtcgcctgct ggcccatccg ctgatccatc cgctgcaggc ccggcgcgag gtgcccgcgg
     2401 ccgaggaagc cgggccacgc gagtcggagt ccaaccggcg cctgacggtt cagggcgcca
     2461 ctctgcacaa cctgcagcga gtgacgatcg acgtcccgct caagcggctg gtcgcgatca
     2521 ccggcgtcag tggctcgggc aaatccacgc tcgcacgcga tgtgttgctg gccaacctgc
     2581 agttcatcaa cgtgaagggc ggcgcaccga aatggcaggg ttgcaacagc atcgagggct
     2641 ggggttacat cgaccgcgtg ctcgaggtcg accagacgcc gataggcaag acgcctcgca
     2701 gttgcccggc cacctacatc ggcttctggg acacgatccg caagctgttt gccgacacgc
     2761 tggagtcgcg tgcgcgcggc tacgctccga accggttcag cttcaacacc ggcgagggcc
     2821 gctgcccggc ctgcgaaggc cagggcatgc gcacgatcga gatgagtttt ctgcccgacg
     2881 tgaaggtgcc ctgcgatcag tgtcacggac agcgcttcaa cccggaaacg ctggcggtca
     2941 cctggcgcgg ccgcaacatc ggcgaggtgc tcggcatgga gatcgacgag gcggtcgact
     3001 tcttcgccag catgccggcg gtcagtcacc cgctgcggct gatgaaggac gtgggcctcg
     3061 gctacctgac gctcggccag ccctcaccga ccctgagcgg tggggaggcg cagcgcctga
     3121 agctggtcac ggagctgacc aaggtgcgcg acgatgtcgg caagcggggc aataaagcgc
     3181 cgcacacgct ctatgttctc gacgagccga cggttggcct gcacatggcc gatgtcgaga
     3241 agctgatacg cgtgctgcac cgactggtcg atggcgggca cagcgtggtg gtcatcgagc
     3301 acgacctcga cgtgattgcc gaagcggact gggtgatcga cctgggcccg gaaggcggca
     3361 cgcagggcgg gcgtgtggtt gcagccgcca cgcccgagga tctggcgaac cgttccgaca
     3421 gccacacggg cgtcgcgctg cggccggtcc tcggacgccg gcctggccag tgaaacagcg
     3481 cgcggtcaat cgcctcgctg tccagatcca cccacggtgt tcgcagacgc gatcggcgat
     3541 gagggcaggc tcgcggctac cgcgagtgcg tcagacggtc gcgccctgtt tcgccgtcgc
     3601 ggcagccggt gcagaagtgc ggatcaggtg atcgaaggcg ctcagcgcgg ccttcgcgcc
     3661 ttcgccggtg gcgatgatga tctgcttgaa cggcgtcgtc gtcacatcgc cggccgcaaa
     3721 gacgcccggc accgaagtct ggccgcgggc atcgaccacg atctcgccgt gccgcgacag
     3781 ctcgagcgtg cccttgagcc actcggagtt gggcaccagt ccgatctgga cgaacacacc
     3841 ttcgagattg atgtgatgcg acgcgcccga ggggcggtcg gtgtaggtca agccgttgac
     3901 cttggctccg tcgcccgtga tctcggtggt ctgcgcctgg gtgtggatgg tgacgttcgc
     3961 gaggctgttc agcttgttga ccagcacggc gtcggcgcgc aactgctcgc cgaactcgat
     4021 gagcgtcacg tgaccgacga tgcccgcgag atcgatcgcc gcctcgacgc cggagttgcc
     4081 gccaccgatc acagcgacct tcttgccctt gaacagcggc ccgtcgcaat gcgggcagta
     4141 ggccacaccg tggttgcggt attccttttc gcccggcacg ttgacttcgc gccagcgcgc
     4201 gccggtcgac aggatcacgg tcttgctttt cagcgaaccg ccgttggcca gctgcacctc
     4261 gatcaggcca ttcggctgcg cggccgggat cagcttctcg gcgcgctgca ggttcatgat
     4321 gtcgacgccg tactgccggg cgtgctgctc gagagccatg gcgaacttgg gcccgtccgt
     4381 ctccaggacc gaaatgaagt tctcgatgcc cagcgtgtct agggtctgac caccgaaacg
     4441 ctcggccatc acgccggtgc ggatgccctt gcgcgccgcg tacacggccg ctgcggcgcc
     4501 agcaggccca cccccgacga tcagcacgtc atagggggcc ttcgcgctga tcttgactgc
     4561 atcgcgtgct gccgcgccct tgtcgagctt cgacacgatc tcttcggcct ccatgcggcc
     4621 ctggccgaag tgctcgccgt tcaggaacac catcggcacc gcaagcacct gacggtcctc
     4681 gacctcctgc tggtacaggc cgccatcgat cgtgacgtgc tggacgcgcg ggttcagcac
     4741 cgccatcagg ttgagcgcct gcaccacgtc cgggcagttg tggcagctca gcgaaacata
     4801 ggtctcgaag cggaaatcgc cgtcgaggtt cttgatctgc tcgatcaggt cggcctcgac
     4861 cttggccggg tggcccccga cctgcaacag ggccagcacg agcgatgtga attcatgacc
     4921 cagcgggatc gcggcgaagc ggatgcgcgg cgtctctccg atgcgggcca gcgtgaacga
     4981 cggcttgcgg gcgtccgtgc cgtcggttct caaggtgatc ttgtcgcaca ggctgatgat
     5041 gtcttccagc aggccgtgca tttcgcgaga cgcgtcgctg tcgtccagcg acgccacgat
     5101 ctcgaacgga atcttcacgc gctcgaggta ggcctgcaac tgagccttca gggtgtcatc
     5161 cagcatgggg tgtctccttc ttgtacggtg acgaatgcaa acgccttgat cggacgtcat
     5221 tcaggtccgc cgccagcttt gtgggctcgc ggcggatgaa aaccacccga tgaggttcac
     5281 cgggtggtcg catgcgatcg gaatgcccga tcagatcttg ccgacgaggt cgagcgacgg
     5341 ggtcagtgtc ttggcgcctt cgctccactt ggctgggcag acttcgcccg ggtgcgattc
     5401 cacgtactga gcggccttga tcttgcgcag cagttccttg gcatcgcggc cgatgcccag
     5461 gtccagcact tccagcacct tgatctggcc ggccggattg atgacgaacg tgccgcgcag
     5521 tgcgaggccg gcttcctcga tcatgacttc gaagttgcgc gacagcgtgc cggtcgggtc
     5581 gccgaccagc gggtacttga ccttcttgat cgccggcgag gcgtcggccc agcccttgtg
     5641 ggtgaagtgg gtgtcggtcg acacgccgta gacctcgacg ccgatcttct ggaattcggc
     5701 gtagttgtcc gccaggtctt ccagctcggt cgggcagacg aaggtgaagt cggccgggta
     5761 gaagacgatg gccgaccact tgcccttcag ggtgtcctga gtgatgtcga tgaacttgcc
     5821 gttttgatag gcggttgccg agaaaggctt gatttgggtg ttgatcaatg acatgagggg
     5881 tctcctgttg cgatgattcg ccgttgagtg gccatcaaat gataggtgcc ctaattcatg
     5941 ttctttatga attggggaaa tcaaagcgat tcgcttttcc tatcgatggg gacgggcgtt
     6001 tcaccgccct gtcaaagtcg gttcagcaac gccgccacgg ccaggccgat cgttccgacc
     6061 gccgtctgac ggtgcacccc ggcccgcctg agtcccagca gtcgagccgg catcggctcc
     6121 cggcgtgcct gaacgaaagt gtccaggatc cgcaggttct cctcggtcaa ccgagacctg
     6181 acgcgctcca gcaaccccac attcgcgtct acccagccct tgaactgacc gcccagcagc
     6241 atccgcatcc gctgccagcg cccgcgcagg ctcacgttcg atccgctcag gttctcatcg
     6301 tgctgccggt agcgcacgct gggccagggg tcgtagcgca cctgcccacc gcagccgctg
     6361 accagcaggt aggccagcca gtccggagcc accgcatcgc cctcgcggtt ggcctggatc
     6421 agcaagtcac gcgtcgcgcg gttgaacacc atcgtgttgc cgcctgcgat gctctgcaca
     6481 agcgcgttcc ggaagcaggg tggccgtgtg aagatcggcg acatcccgat gggccggttc
     6541 tcctcatcgg tcagcagcgt tcggctgccg tacagcgcgg gggtgcctgc tggcacggac
     6601 tgcaaccagg ccagcgcacg ttcgagtttc tcgggctccc acacgtcgtc ctggtcgcac
     6661 catgcgtagc cgtccgcgtc gatctccgcg tggcaggtca gcgaccggaa attggcggca
     6721 aagccccgag ccggacccgt caggatggac aggcgatcgc tgccccaccg ggatcgatac
     6781 tgctccagga tcgccaccgt gccgtccgtc gagcggtcgt ccgacaccca cagcgtccag
     6841 ccgcgatggg tctgtgctgc gatcgaatcg agctgctcgg ccaggtggcg ctcgccctgg
     6901 tagctgcaca gcagcaccgc gacgacaggc gcgagcggcg gaagggtctc gttctgggcg
     6961 ggctgcatac ggggcgctga gtatcgcaag cgcgaagccg gaagtcgtga ccatcgactt
     7021 gcccgtcgtg cgcgctccgc ctgatcgtgc gcgtgcaccg ggaagccata atcggccgga
     7081 tgaccagcaa tgacgtgcct ttgcacgtgt atcgacgcga aatctcgact tccgagcaga
     7141 cctcgctgtc ggtcatcgcc ggtctgatcc agccgggttg ttcggtgctc gatctgggga
     7201 ccggcagcgg cgcgctgggg cggcatctcc gtgaacagca ggcgtgcgtg gtcgatggcg
     7261 tcacgctgaa cgatgccgaa gccgagcttg cccggccgca ctaccgccgc atcgaagtgg
     7321 ccgacttgga gcaggcgccg ctcacggaac tgtttcaggg tgcggcctac gactgcatcg
     7381 tctgtgcaga cgtgctggag cacctgaagc agcccgaaca ggtactgagc gcctgccgtg
     7441 aattgctggc tccgacaggg aaggtgctga tctcggtgcc gaacgcgggc tattgcgggc
     7501 tgatcgccga actgctcgag ggcgagttcg aataccggcc cgagggtctg ctcgacagca
     7561 cccatctgcg cttcttcacg cgccgctcgc tggggcggtt cctggcgagc gaaggctggt
     7621 cgatggtgtc cctgcttccc gtgctcaggg aattgggtgc ctcggagttt cgaacccgct
     7681 tcgacagcct gccgcccgca gttggtcgct atctgctggc cgtgccggac gcgctggcct
     7741 atcagttcgt cgcggaagtc cgccccgcca cgagcgcttc actggctgtc cccgtcgaca
     7801 gcgagccgac gcagccggcg gcccaggcgc tcttcacggc ggaactgttc cttggccagt
     7861 cgggagcgta ctcgcagcag cacaagcagg tggttgccgg cgtgatcggc aggagccgtc
     7921 agacgttgcg ctttgcgtgg ggcgatgacg ttcccgcatg gacctcgatc aagctcgatc
     7981 cggcagatcg cccggggttc atccatctgt accgcatcag tctgatgtcc tccaacggcg
     8041 agtgtgtctg ggaatggcgc tgcgaacggg actccgtgtc ctcgctcgag gcgtcggcct
     8101 cgcagcagtt gctgctgcgt ccgccctggc ccgaaggtgc ccccgcgttg gcgctgctgc
     8161 acggtgacga tccctggatc gaactaccga tcgcacctgc cgcactggcc gcgatgggca
     8221 gccgtcacgg tggagcgctc gaggtggagg tcggctggcc gatgtcggcc gattacctcg
     8281 ctctgaaaga tgtcgtgaag acggtcgacg atcgcctgaa gtcggtccag aagcacctcg
     8341 atcagcaggt cgaggagcgc gatcatctgg cgcgtgaagt ggtatccctg gcgcaggtcg
     8401 aaagcaggct ctcggctcaa cgggacgaac tcctcaagca gaagggcctg ctggtcgacg
     8461 agcagaccga gctgcgcaac cagcatttca agctccggga tgactatgcc gaactggctg
     8521 cccacctgca ggacatcgag cgttccaggt tgttccggat gacgcgcccg ctgtcgcatc
     8581 tgaagggttg ggtcgatcgc aacctgcggg gacggcctgg ccgcccgaat gctgtggtcc
     8641 aggcgactgc actggtcagc acgccggtca ccccgacacc gtgtcctgtc gatgtcatcg
     8701 tcccggtcta tcgggggttg gccgatacgc aactctgcct cgagtcggtg ctggcctcgc
     8761 gcaacgacag cgcattccgc ctgatcgtca tcaacgacgc gagccccgag ccggaactgg
     8821 cgggctggtt gcgcgagctt cgggaccgcg accagcgcgt cgttctggtc gagaacgaac
     8881 agaacctcgg tttcgtcggc acggtgaacc gtggcatggc gatgagtgat gcccacgacg
     8941 tgatcctgct caacagcgac acggaggtcg cgaacgactg gctggatcgc ctcaagcgtg
     9001 cggcttattc ggatgcccgg gtcggcaccg tcacgccgtt ctcgaacaac gcgaccatct
     9061 gcagctaccc caggttctgc gagagcaaca cgctgcccgc cggactgagc accgcagcgc
     9121 tggatgcctt gttcgcgcgc acgctcgcgg gccagacgat cgacgttccc accggcaacg
     9181 gtttctgcat gtacgtgcgc cgagactgtc tggcgcaggt ggggttgttc gatgtcgagc
     9241 agttcggcaa gggctatggc gaggagaacg acttctgcca gcgcgcccag gaggccggct
     9301 ggcgcaacct gcatgcgctg gacacgtttg tgttgcattc cggcggagtc agcttcggag
     9361 cgtccaaggg cgcacgcgag atcgcggcca tggagaccct gcgtcgcctg catcccgcct
     9421 acgagcagca ggttcatcgc ttcatcgcgc tcgatcctgc cctgccggca cgccaggcgg
     9481 tcgatctggc ccgcgtctcc gcggccggca agcccgtcgt cctgctggtg atgcacgat
//
LOCUS       DAOORV010000130         9290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9206_length_9290_cov_6.758852, whole genome shotgun sequence.
ACCESSION   DAOORV010000130 DAOORV010000000
VERSION     DAOORV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_9290_cov_6.758852"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /gene="hrcA"
                     /locus_tag="PKV56_14385"
     CDS             complement(<1..976)
                     /gene="hrcA"
                     /locus_tag="PKV56_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOX69049.1"
                     /translation="MMDERARTLLKTLVERYIADGQPVGSRTLSKASGLELSPATIRN
                     VMADLEDLGLIASPHTSAGRIPTARGYRVFVDTMMTAQPRQFDAPSFGSQLQADQPQR
                     VIANAAQMLSSLSNFVGVITAPRKASVFHHIEFMRLSERRVLVIMVAPDGDVQNRVIF
                     TAKDYTQAQLLEASNFLTANYAGLTLEDVRERLKTEVDALRGEISTLMQAAVQAGSEA
                     MADSQEQVVISGERNLLTVQDFTSDMGSLRKLFDLFEQKTQLMRLLDVSSRAEGVRIY
                     IGGESRVVPFEELSVVTAPYEVDGRVVGTLGVIGPTRMAYDRMIQIVDI"
     gene            1043..1924
                     /locus_tag="PKV56_14390"
     CDS             1043..1924
                     /locus_tag="PKV56_14390"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOX69050.1"
                     /translation="MKSRFRHAALVGKYNALGSRDVLEEVARFLILQGLDVSMEYETA
                     LNTGLTEYGALTPAELGKQCDIAVVVGGDGTMLGIARQLARYGIPLVGINQGRLGFIT
                     DVPLGEFASALAPMIAGSYEEEHRTMLEGGVCRDGEVIFEAFALNDVVVSRGATSSMV
                     ELKIQIDDEFVSNLRADGLIIASPTGSTAYALSAGGPILHHGIAGLVLVPIAPHDLSN
                     RPIVLPDSGEISIEIVAGRDASVNFDMQGLASLLHGDRITVRRAPYQARFLHPPGWSY
                     YATLRRKLHWNSGGQLG"
     gene            2030..3721
                     /gene="recN"
                     /locus_tag="PKV56_14395"
     CDS             2030..3721
                     /gene="recN"
                     /locus_tag="PKV56_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOX69051.1"
                     /translation="MWRRLVLRDFVIVTTLDVDLSEGFSVLTGETGAGKSILVDALQL
                     ALGSRGDAGVVREGASRCEISAEFDTPGSLRGWLSDAGFDGEDTLLLRRTIDIQGKSR
                     AWINGSAATIAQLREVADHLVDIHGQHAWQSLTRAPAVRGLLDAHAGVSTQALGAAWA
                     DWRAATEALERAQTQQADIERERERLAWQIGEVDKLAPGADEWAELNAEHTRLANVQT
                     LLEAARDALNAISDLDESATTLTGRAIEHLRHAAEIDNELADVSDVLRGAQAQLQDAA
                     HTLSGFLNGTDTDPERLSELDERMSAWMGLARRYRRPPADLAALLAQWKDELQALDAA
                     ADLEVQRTRVAHARSAYETEAARVSAGRRRAAPKLGEAVTQAMQQLGMVGGRFEVALT
                     AQESPQSFGMESAEFLVAGHAGSTPRPLGKVASGGELSRIALAIAVTTSALRRSDATA
                     GTLVFDEIDAGVGGAVAETVGRLMKQLGAERQVLAVTHLPQVAACADHHHVVSKITED
                     GRVRSEVSVVSGEVRVAEIARMLAGERLSGTSLAHAQEMLTVGSDMPASPGQESR"
     gene            3718..4701
                     /gene="rapZ"
                     /locus_tag="PKV56_14400"
     CDS             3718..4701
                     /gene="rapZ"
                     /locus_tag="PKV56_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOX69052.1"
                     /translation="MNSTGAAPKAVQDDNDPDASQREIVLVTGISGSGKSVALHALED
                     AGFFCIDNLPPELLRDFLRLRHAHMDHRVAIAVDVRSAGSLPHLLPLMNQLSQEGVRV
                     RSIFLDASTDALVRRFSETRRPHPLSQGPEHHQAEDTGYTVPADLDHGIAADGHRALI
                     DAIELERELLSELREASTVIDTSQLRPAQLRIWIRDLMSASSNRLTLVFESFAFKHGV
                     PLDADFVFDVRVLPNPHYIPELRPQDGRDAGVVAYLEAQSEVGEMLEQIDAFLTRWLP
                     AFNRDQRSYLTVAIGCTGGQHRSVYLVETLARRFARRTVTLVRHRELDARD"
     gene            complement(4726..5802)
                     /gene="mutY"
                     /locus_tag="PKV56_14405"
     CDS             complement(4726..5802)
                     /gene="mutY"
                     /locus_tag="PKV56_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOX69053.1"
                     /translation="MKPDSGECALRELRSGFAATVIDWQRTHGRHGLPWQHTRDPYRV
                     WLSEIMLQQTQVVTVLEYYERFLQRFPDVAALAAASQDEVLGLWSGLGYYSRARNLHR
                     CAQAVASQHGGTFPQSSQELQTLPGIGRSTAAAVAAFCFGERVAILDGNVKRVLTRLL
                     GFDGDLSLAAQERALWAEATALLPLQGVEAYTQGLMDLGATLCSLRSPQCLLCPVRSM
                     CTAARSGTQQSYPVKSLKLKRSRREHIWLWLSWRNQVWLVKRPDRGVWAGLWSLPELP
                     ERSALQQITVGWPGDAECLPSFVHTLTHLDWTLHPVRWSLPASATASQVDDCTAAFPD
                     GRWFDLKDAMAAGLPAPLRKLLSA"
     gene            complement(5855..7879)
                     /locus_tag="PKV56_14410"
     CDS             complement(5855..7879)
                     /locus_tag="PKV56_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOX69054.1"
                     /translation="MTSAFAGRFDEISLWRDAMRAGLDDVSLFLSEQGLVDEGLNALV
                     GALRERLSAEKLVVAFVAEFSRGKSELINAIFFADTGRRVLPATPGRTTMCPVELAFD
                     AKEPVTLALLPIATRLAGASLSELRRQPEAWTHVPLDVAQPEQLSNALCEVMGTRRVA
                     IDEARALGLWDESNPEDNPVPDDEGLVEVPAWRHALINYPHPLLQQGLVVLDTPGLNA
                     LGAEPELTLGLLPSAHATVFIVGADTGVTKSDMAIWRDHLGGRQGATFVVLNKIDALL
                     DPLLTPAQVAAHIEAQRRSAASILGVEPDRVFALSARQALVARVEGDAQGLAVSRLGE
                     FESALSAELLPHRQQVLRHLIEQGVGQIQRQAARLMGDSRRQIAEQLLELRGLRGKSG
                     AKLKLIKLRVEEEAVEFEHSHSTLLALRGMQARMIDVALEALSSAGLEAEVDRAQAEM
                     RSTLLGLGARKAFVALSSRLADRLRKARSQMDEVREMLGGTFSRLNAEFGFGLVLTPG
                     PELSRFIDELGSIERSYAQYLGLGQSVRLVQPQFMSQFRRMLMGRLSVVWDGARGDIE
                     RWSGTTTTHVEVQLHERRRGFQKRAEALSRIRNASGDLEGRLAELEQQDAQLQRQLEQ
                     LVAVTQSVLSRAAGNSASPPRVAQQAGEPEGPVDLDLPMDEFVSVRRARA"
     gene            complement(8030..8875)
                     /gene="mutM"
                     /locus_tag="PKV56_14415"
     CDS             complement(8030..8875)
                     /gene="mutM"
                     /locus_tag="PKV56_14415"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOX69055.1"
                     /translation="MPELPEVEVTRQSFSDRIEGAVVTGVRLGKPLRWPLGIDPTRLL
                     DRPVGAVTRRGKYLWLPLGSAVSRTFDRDDGGLLLHLGMSGSLAFNSDPEVPGLHDHF
                     DLVTDRGTLRLTDPRRFGAVVWSDALDEGMAAKLLSTLGLEPFDAAFDGTYLHAALKR
                     RRVAVKLVLLGGDIVVGAGNIYACEALFSAGIDPRTRSDRISRPRCERLAQSIRRTLQ
                     EALELGGSTLRDFRDAHGMAGQFQSRARVYGRAGEACHVCGVPIRRIVQGQRSTFFCP
                     NCQKR"
     gene            9065..>9290
                     /locus_tag="PKV56_14420"
     CDS             9065..>9290
                     /locus_tag="PKV56_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69056.1"
                     /translation="MAPTPAPAGVNNSDLDASLFYQLLIGELELTASEGSTAVLVMLD
                     AANKTKNEQIYKRATDMALQIRAGSQALSVV"
BASE COUNT         1593 a         3117 c         3127 g         1453 t
ORIGIN      
        1 tgatgtcgac gatctggatc atgcggtcgt aggccatgcg cgtggggccg atgacgccca
       61 gcgtgcccac cacgcgaccg tccacttcat agggcgcggt gaccacgctg agttcctcga
      121 agggcaccac gcggctctcg ccgccgatgt agatgcgcac gccctcggcg cggctgctca
      181 cgtcgagcag gcgcatcaac tgcgtcttct gctcgaacag gtcgaagagc ttgcgcagcg
      241 agcccatgtc gctggtgaaa tcctgcacgg tcagcaggtt gcgctcgccg gagatgacga
      301 cctgctcctg gctgtcggcc atcgcttcgc tgccggcctg caccgcggcc tgcatcagcg
      361 tggagatctc gccgcgcagc gcatcgacct cggtcttcag gcgctcgcgc acgtcctcga
      421 gggtgagccc ggcgtagttg gcggtgagga agttgctggc ctcgagcaat tgcgcctggg
      481 tgtagtcctt ggcggtgaag atgacgcggt tctgcacgtc gccgtccggc gcgaccatga
      541 tcacgagcac gcggcgttcg ctcagccgca tgaactcgat gtggtggaag acgctggcct
      601 tgcgcggtgc cgtgatgacg ccgacgaagt tcgacaggct cgacagcatc tgggccgcgt
      661 tggcgatgac gcgctggggt tgatcggcct gcagctgcga gccgaacgaa ggcgcgtcga
      721 actgcctcgg ttgcgccgtc atcatcgtgt cgacgaagac ccggtaaccg cgggcggtcg
      781 gtatgcgacc ggcgctggta tgcgggctgg cgatgagccc gagatcctcg aggtcggcca
      841 tcacgttgcg gatggtggct ggcgaaagct cgagcccgga ggccttcgac aaggtgcgcg
      901 agcccaccgg ctgaccatcg gcgatgtagc gctcgaccag tgttttgagt aaggttcttg
      961 cgcgttcgtc catcactttt tcattgtagg gcgaccctgg tttcgccccg ttccagcacg
     1021 aatcgctgtg gtgtaattcc gcatgaaaag ccggttccgt catgccgcac tggtcggaaa
     1081 gtacaacgcg ctgggaagtc gcgacgtgct ggaggaagtc gcccgcttcc tcatcctcca
     1141 gggtctcgat gtgtcgatgg agtacgagac cgcactcaac accggactca ccgaatacgg
     1201 cgcgctgacg ccggccgagc tgggcaagca gtgcgacatc gcggtggtgg tcggcggtga
     1261 cggcacgatg ctgggaatcg cccgccagct ggcccgctac ggcatcccgc tggtcggcat
     1321 caatcagggt cggctgggct tcatcactga cgtgccgctc ggcgagttcg cgagcgcgct
     1381 ggcgccgatg atcgcgggca gctacgagga agaacaccgc acgatgctcg aaggcggcgt
     1441 ctgtcgcgat ggcgaggtga tcttcgaggc gtttgcgctc aacgacgtgg tggtcagccg
     1501 cggcgccacc tcgagcatgg tcgagctgaa gatccagatc gacgacgagt tcgtttccaa
     1561 cctgcgtgcg gacggcctga tcatcgcgtc acccaccggg tccacggcct atgcgctgtc
     1621 tgctggcggg ccgatcctgc accacggcat cgccggcctg gtgctggtgc cgatcgcgcc
     1681 gcacgacctg tcgaaccgcc cgatcgtgct gcccgattcg ggcgagatca gcatcgagat
     1741 cgttgccggc cgcgatgcca gcgtcaattt cgacatgcag gggctggcca gcctgctgca
     1801 cggcgaccgc atcacggtgc gtcgcgcgcc ctaccaggcg cgcttcctgc acccgccggg
     1861 ctggagctac tacgccacgc tgcgtcgcaa gctgcactgg aactcggggg ggcagctggg
     1921 atgaacctgt cccggcgctc atcccatgcg ctgcgcgcgg aagccggctg gttccagatc
     1981 gcggcactcg gaaagcgtcg atgccttcgg gcggccggga aaaactgaca tgtggcggcg
     2041 tctggtcctg cgcgatttcg tcatcgtcac gacgctggac gtcgatctgt ccgagggctt
     2101 ctcggtgctg accggggaaa ccggcgcggg caaatcgatc ctggtcgatg cacttcagct
     2161 cgcactgggc agccggggcg acgcgggcgt ggtgcgcgag ggcgcgagtc gatgcgagat
     2221 cagcgccgag ttcgacacgc cgggcagtct gcgcggctgg ctgtccgatg caggcttcga
     2281 cggcgaggac accctgctgc tgcgccgcac gatcgacatc cagggcaaga gcagggcctg
     2341 gatcaacggc agcgcggcga cgatcgcgca actgcgcgag gtcgccgacc acctggtgga
     2401 catccacggc cagcatgcgt ggcagagcct gactcgcgcg cctgcggtgc gaggcctgct
     2461 cgatgcccac gcaggggtga gcacgcaggc actgggcgcg gcctgggccg actggcgcgc
     2521 ggccaccgaa gcgctggaac gtgcccagac ccagcaggcc gacatcgaac gcgagcggga
     2581 gcggctggcc tggcagatcg gcgaggtgga caagctggcg ccgggtgccg acgaatgggc
     2641 cgagctgaat gcggagcaca cgcggcttgc caacgtacag acgctgctcg aagccgcacg
     2701 cgatgcgttg aacgcgatca gcgacctgga cgagagcgcg accacgctga caggccgcgc
     2761 gatcgaacac ctgcgccatg cggccgagat cgacaacgaa ctggccgacg tgtcggacgt
     2821 gctgcggggc gcgcaggccc agcttcagga tgctgcgcac acgctgtcag ggtttctgaa
     2881 cggcaccgac accgaccccg aacgactgag cgaactcgac gagcgcatgt cggcgtggat
     2941 gggccttgcg cggcgttacc gccgcccgcc ggccgacctg gcggcgctgc tcgcgcaatg
     3001 gaaggatgaa ttgcaggcac tcgacgcggc cgccgacctg gaggtgcagc gcacccgcgt
     3061 ggcacacgcc cgatcggctt atgaaaccga ggccgccagg gtgtctgcgg gccgccgacg
     3121 tgcggcgccg aagctgggcg aagccgtgac tcaggcgatg cagcaactcg gcatggtcgg
     3181 cggacggttc gaggtcgcgc tgacggcaca ggaatcaccg cagtccttcg ggatggagtc
     3241 ggccgagttc ctggtggcag gtcatgccgg cagcacaccg cgccccctgg gcaaggtggc
     3301 ctcgggcggt gagctgtctc gaattgcgct ggcgatcgcg gtcaccacca gcgcgttgcg
     3361 gcgcagtgac gcgaccgcgg gcacgctggt gttcgacgag atcgatgccg gggtcggcgg
     3421 ggccgtggcc gaaaccgtcg ggcgcctgat gaagcagctg ggcgccgaac gtcaggtgct
     3481 ggctgtcacg cacctgccgc aggtggcggc ctgcgcggat catcatcacg tggtctcgaa
     3541 gatcaccgag gacggccgcg tgcgaagcga agtgagtgtc gtttcagggg aggttcgcgt
     3601 ggccgagatc gcccgcatgc tggcaggcga gcgcctgtcg ggcacgagtc tcgcgcacgc
     3661 tcaggaaatg ctgacggtcg gctcggacat gccggcatca ccaggacagg aatccagatg
     3721 aactcgacag gcgctgcgcc gaaagccgtg caggacgaca acgatccgga cgcatcgcaa
     3781 cgcgagatcg tgctggtcac cggcatctcg ggctcgggca aatcggtggc gctgcatgcg
     3841 ctggaggatg ccgggttctt ctgcatcgac aacctgcccc cggaactgct gcgcgacttc
     3901 ctgcgcctgc ggcacgccca catggatcac cgcgtcgcca tcgcggtcga tgtgcgcagc
     3961 gcgggatcgc tgccgcatct gctgccgctg atgaaccagc tgagtcagga gggagttcgg
     4021 gtccgctcga tcttcctgga tgccagcacc gacgcgctgg tgcgccgctt ctccgaaacc
     4081 cggcgccctc acccgctgtc gcagggcccg gaacatcacc aggcggaaga caccggctac
     4141 acggtgcctg ccgacctgga tcacggcatc gccgccgacg gccaccgggc gctgatcgat
     4201 gcgatcgagc tcgaacgcga gctgctgtcc gagttgcgcg aagcctcgac ggtgatcgac
     4261 accagccagt tgcgaccagc ccagctgagg atctggatac gcgacctgat gagcgccagc
     4321 agcaaccgcc tgacgctggt cttcgagtcc ttcgccttca agcacggcgt gccgctggat
     4381 gccgacttcg tgttcgacgt gcgggtgctc ccgaatccgc actacatccc cgaactgcga
     4441 ccgcaggacg gtcgtgatgc gggggtggtg gcctacctcg aggcgcaatc cgaagtcggc
     4501 gagatgctgg agcagatcga cgctttcctg acgcgctggc tgcccgcgtt caaccgggac
     4561 cagcgcagct acctgacggt ggccatcggt tgcaccggcg ggcagcaccg ttcggtgtac
     4621 ctggtcgaga ccctggcgcg ccgtttcgcc cgacgcaccg tgaccctggt gcggcaccgc
     4681 gagctcgacg cgagggattg atcggcggtc ggccccaccg accgctcagg ccgacagcag
     4741 tttgcgcagc ggtgctggaa gcccggccgc catcgcatcc ttcaggtcga accagcgtcc
     4801 gtccggaaag gccgcggtgc agtcatcgac ctgcgaagcc gtggcagacg cagggaggct
     4861 ccagcgcacc ggatgcaagg tccagtcgag gtgcgtgagg gtgtggacga agctcggcag
     4921 gcactcggcg tcgccgggcc agccgaccgt gatctgctgc agtgcggagc gctccggcag
     4981 ttcgggcagg ctccacaggc ccgcccaaac gccgcgatcg ggccgcttga ccagccagac
     5041 ctgattccgc cacgacagcc acagccagat gtgctcgcgc cggctgcgct tgagcttgag
     5101 gctcttcact ggataggact gctgggttcc gctgcgcgcc gccgtgcaca tggatcggac
     5161 cggacacagc aagcactgcg gcgagcgcaa gctgcacagg gtcgcgccca gatccatcag
     5221 gccctgcgtg taggcttcga cgccctgcag cggcagcagg gccgtggcct cggcccacaa
     5281 ggcgcgctcc tgagcggcca gcgacaagtc gccgtcgaac ccgagcagcc gcgtcagcac
     5341 gcgcttgacg ttgccgtcga ggatggcgac acgctcgccg aagcagaacg ccgccacggc
     5401 agccgccgtc gaacgaccga tgccggggag cgtctgcagt tcctggctgg actgcggaaa
     5461 cgtgccgccg tgctgcgacg ccacggcctg cgcacagcga tgcaggttgc gcgcgcggct
     5521 gtagtagccc agcccgctcc acagtcccag cacctcgtcc tgtgacgccg ccgccagggc
     5581 cgcgacatcg ggaaaacgct gcaggaagcg ctcgtagtac tcgagcacgg tcaccacctg
     5641 cgtctgctgc agcatgattt cggacagcca gacgcggtag ggatcgcgcg tgtgctgcca
     5701 cggcaggcca tggcgtccgt gggtgcgctg ccagtcgatg accgtcgcgg caaatcccga
     5761 tcgcaactca cgcaaggcgc attcgccaga atcgggcttc accgcgatcc ggcccgccag
     5821 aggcgccctg ccgagggcgc gcacgggtct gaagtcacgc ccgggcgcgt cggaccgata
     5881 cgaactcgtc catcggcagg tcgaggtcga caggtccttc cggctctccg gcctgctggg
     5941 caactcgcgg cggggacgcc gaattgccgg cggcgcggct gagcaccgac tgcgtgaccg
     6001 ccacaagctg ctccagctgc cgttgcagct gcgcgtcctg ttgctcgagt tcggccaggc
     6061 ggccctcgag gtcaccggat gcattgcgga tgcgcgacag cgcctccgcg cgcttctgga
     6121 atccgcggcg gcgttcgtgc agctgcactt cgacgtgggt ggtcgttgtg ccgctccatc
     6181 gctcgatgtc gccgcgtgcc ccgtcccata ccacgctgag gcgccccatc agcatgcggc
     6241 ggaattgcga catgaattgc ggctgcacca gccgcaccga ctgaccgagc ccgaggtact
     6301 gcgcgtagct gcgctcgatc gatcccagct cgtcgatgaa tcgcgacagc tccggccccg
     6361 gcgtcagcac caggccgaaa ccgaactcgg cgttcagccg ggaaaacgta ccaccgagca
     6421 tctcgcggac ctcgtccatc tggcttcgcg ctttcctcag ccgatcagcc agccgagacg
     6481 acaaggcgac gaaggccttg cgggcaccca gaccgagcag agtgctgcgc atctcggcct
     6541 gcgcacgatc gacctcggcc tccaggccgg cactcgacag ggcctcgagc gcgacatcga
     6601 tcatgcgtgc ctgcattcca cgcaaggcca gcagcgtcga atggctgtgc tcgaactcga
     6661 ccgcctcttc ctcgacccgc agcttgatca gcttgagctt cgcgccgctc tttccgcgca
     6721 atccacgcag ttcgagcagt tgttcggcaa tctggcgacg gctgtcaccc atgaggcgcg
     6781 cggcctggcg ctgaatctgc ccgactccct gctcgatcag gtgccgcaga acctgctggc
     6841 ggtgcggcag caattcggcg gacaacgcgg attcgaactc gcccagccgg ctgacggcca
     6901 gcccctgggc gtcaccttcg acccgcgcga ccagcgcctg acgcgccgaa agcgcgaaga
     6961 cgcgatccgg ctcgacaccc aggatgctgg cggcggaacg tcgctgcgcc tcgatgtgcg
     7021 ccgccacctg tgcgggggtg agaagcgggt cgagcagcgc atcgatcttg ttcagcacca
     7081 cgaacgtcgc cccctggcga ccaccgaggt ggtcacgcca gatggccatg tccgacttgg
     7141 tcacgccggt gtccgcaccg acgatgaaca ccgtggcatg cgccgaaggc agcaggccga
     7201 gggtgagttc aggttcggcg cccagcgcat tgagtcccgg cgtgtcgagc accaccagtc
     7261 cctgctgcag caggggatgc ggatagttga tcagcgcgtg tcgccaggca ggcacctcga
     7321 ccagcccctc gtcatccggc accgggttgt cttcggggtt cgattcgtcc cacaggccga
     7381 gcgcacgggc ctcgtcgatg gccactcgcc gggtgcccat cacttcgcac agggcgttcg
     7441 acaactgctc gggctgggcc acatcgagcg gcacatgcgt ccaggcctcg ggctggcggc
     7501 gcagctcgga caacgaggcg ccggccaatc gcgtggcgat cggcagcagc gccagcgtga
     7561 cgggctcctt ggcgtcgaag gccagttcca ccgggcacat cgtcgtgcgg ccgggcgtgg
     7621 ccggcagcac ccgacgcccg gtatcggcaa agaagatcgc gttgatcagc tccgacttgc
     7681 cgcgcgagaa ctccgcgaca aaggcaacca ccagcttctc ggcagacagg cgctcgcgca
     7741 aggcgccgac gagcgcgttc aaaccctcat cgaccagccc ctgctcggac aggaacaacg
     7801 acacgtcgtc cagcccggct cgcatggcat cgcgccagag gctgatctca tcgaagcggc
     7861 cagcgaaggc tgaagtcatg gcggttggta tccgaagtag atggttcggg gtcggccgca
     7921 ccgactgcga ctgtccatcg aattcatcct aatccaatga aaatcgcggc catccgcaga
     7981 aacgggctgt aactccccgg caattgaccg gttgccgcgg gcggttgcgt cagcgcttct
     8041 ggcaattcgg gcaaaagaag gtcgagcgct gtccctgcac gatccgccga atcggcacgc
     8101 cgcacacgtg gcaggcctcc ccggcacggc catacacgcg ggcccgcgac tggaactgcc
     8161 ccgccatgcc gtgcgcatcg cggaaatcac gcagggtcga tccaccgagc tcgagcgcct
     8221 cctggagcgt ccgccggatg gactgcgcca gacgttcgca acgtggccgg ctgattcggt
     8281 cgctgcgggt gcgtggatcg atgccggcac tgaacaacgc ttcgcaagcg tagatgttgc
     8341 cggcaccgac cacgatgtcg ccaccgagca gcacgagctt gacggccacg cgtcggcgct
     8401 tcagagcggc gtgcaggtag gttccatcga aggcggcatc gaagggctcg agccccagcg
     8461 tcgaaagcag cttggccgcc atgccctcgt ccagtgcgtc agaccagacc accgcgccga
     8521 aacgccgggg atcggtcaag cgcaacgtgc cgcgatcggt caccaagtcg aaatggtcat
     8581 gcaagccagg cacttcggga tcgctgttga aggcgagcga gcccgacatg ccgaggtgca
     8641 gcagcaagcc cccatcatcg cgatcgaacg tgcgactcac cgcagacccc agcggcaacc
     8701 agaggtactt gccacgacgc gtcaccgctc cgaccggccg gtcgagcaga cgcgtcggat
     8761 cgatgcccag cggccaacgc agaggcttgc ccaggcgcac gccggtgacg acagcaccct
     8821 cgatgcgatc ggaaaaactc tggcgggtga cttcgacctc gggaagttca ggcatgggag
     8881 gaattatccg gaggctgcgc ggtcgtacca cccgaggtcg cctcgaacct caccgctcta
     8941 gaatcatttt cgtctccagg agttgccgat gccagcacgt ctgtccacct cgcaagcggt
     9001 ttgcgcgctt gtcgttgctg gttgcatttg ggcgctgccc gcgcacgttc aggccgcatc
     9061 caagatggct ccgacgcctg cgccggccgg cgtcaacaac tcggatctcg acgcatcgct
     9121 gttctaccag ttgctgatcg gcgagctcga actcaccgcc agcgaaggta gcaccgccgt
     9181 tctggtgatg ctggacgcag cgaacaagac caagaacgag cagatctaca agcgtgcgac
     9241 cgacatggca ctgcagatcc gtgccggctc gcaggcgctg tcggtcgtgc
//
LOCUS       DAOORV010000131         9245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9263_length_9245_cov_9.149619, whole genome shotgun sequence.
ACCESSION   DAOORV010000131 DAOORV010000000
VERSION     DAOORV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9263_length_9245_cov_9.149619"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKV56_14425"
     CDS             <1..1065
                     /locus_tag="PKV56_14425"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOX69057.1"
                     /translation="CRDVVVEVTKAGGFGVLGAVTHSPEQLERELKWIDEHVGGRPYG
                     VDVLIPVVYDREAEASTEEVIDLIPAEQRQFVDKLLDAEGVPPLSADDAAEARRELIG
                     RGRNTTPLGARRLIDVALRHPQVKLVVSALGAPPKELVDELHGRGILVGSLCGKAEHA
                     KAQRAVGVDIIVAQGTEAGGHTGQIATMVLVPQVVDVAGPDIAVLAAGGISRGRQIAA
                     AFALGAQGVWTGTIWLGTRESELTPMEKEVLFKAKSEDAVQRKWMTGKTVRMIRSKAS
                     EAWEQPGAPRHLKPPLQNILYLGARIRIEKAHRDDLCSFPAGQVVGNMKEETSVRQVM
                     QDLMQEYIDTAERISPLINL"
     gene            1074..>1563
                     /locus_tag="PKV56_14430"
     CDS             1074..>1563
                     /locus_tag="PKV56_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOX69058.1"
                     /translation="MDKTRIPVIVGTGQVTDTTSKPEEGRSPLQLMHDAVKLAAADSG
                     AQDQGAALLRGLDSVTVIRLFADTLPRFASPFGKLANAPWSIAQRVGAANATDLVCPP
                     QGGDSLVMLARACERIAQGESQAALVVGGEALRTELAAKRAGLQLQWGEDAPTTPNQL
                     TGV"
     assembly_gap    1564..1663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1664..2724
                     /locus_tag="PKV56_14435"
     CDS             <1664..2724
                     /locus_tag="PKV56_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOX69059.1"
                     /translation="DMYTKAEEKHGMRSAIAMYALIGQALRHAAGQTVDQYREASAKL
                     FARFAAVARDNPLATRRKGYSAEQIAEVNAENPFVGFPYTKLMTASAFVDQSAAFIVV
                     SEARADELGIPQDKRVYLHGEAHAHNTWFVSDRERLDQSPAMRLTSRAALAQAGKTIA
                     DVDVFDIYSCFPSVVQIACKELGISPDDKRGLTVTGGLPYFGGPGNNYVTHAIAEMVQ
                     RVRAKPGAYGLVMANVGLVTKESVGLFSTERPNKPFMRADSDAIQREIDAQTKVRFTE
                     APQGDATIETYAVLHGKNGPESGVLFGRLAATGERFVANTPADAATLAKLEQVEGIGL
                     PGTVTQVDGRNVFTPRFG"
     gene            2730..4853
                     /locus_tag="PKV56_14440"
     CDS             2730..4853
                     /locus_tag="PKV56_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOX69060.1"
                     /translation="MAAPDTGLARLFNPRSVALVGATDRSTWSKMAFDNLKLLGFEGK
                     VHLVNRSGGVVHGQQSFKTAVDIGESVDVALLMVPNPAMEDALRDLGAAGIRHAVVLA
                     GGFAETGSEGRTMQERMVATARELGITLLGPNCLGFINFTTGAVCWTGAMRTPPLKGG
                     ISIVSQSGAVATVMKHFAHQHGVGMNVVAATGNEAMLGLAECVDYLVDDESTKVIAVF
                     AETVRDTRLFRAAAERALAKAKPIVVLKVGRSDIATQAAQSHTGSLVGDDSVFDGVCR
                     QLGLVRVRSIEELVFTAALLEKTGVLAEGGVAVLSSSGGMGELAADYAQLETLRLPAL
                     SNETLSALREILPPMATPANPLDLTGAVVNDWALFTRCMDAMQRDPAVSVLVCVADVP
                     TANNNDWAPLAVGTLQAIGQFKPDGRARYLVVSNAVKAVSDKSREEVEKAQIPYLACG
                     LDIALRALRYAADWSRMSRSAGGERAARALPGAASTAGADLPQSERETVDYLKRFGVP
                     VVPQVLATDAKQAVAAARDMNGPVVLKIASPDIAHKTEVGGVVLNLQGDDAVEAAFRR
                     IMDAAASAMPKARLDGVIVSPMRKGGIELFTGVRVDAQWGPVIALGLGGVWIEALQDV
                     SLRLLPVTPADVEQMVSELRGAKLLQGFRGAPPVDVAKLADAVARIGDAALALGATLD
                     TLEVNPLLADGARIEALDALATYNH"
     gene            4866..5657
                     /locus_tag="PKV56_14445"
     CDS             4866..5657
                     /locus_tag="PKV56_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOX69061.1"
                     /translation="MAITQREFCTVEKDGHLTIVTINRPDVMNSTHYEADLELDQVWD
                     EFAEDPEQWVGIITGAGDRAFSTGNDLKMHAKRGVRQIPKGGFAGLTTRFDLDKPVIA
                     AVNGIAMGGGFELALACDLVIAAENAFFALSEPRVGLSALAGGVQYLPRAIGLPRAMG
                     ILLTGRRVPAKEGYELGFVTAVAPAGGAMEEARKWAAQMLECSPLALRATKEVARKTM
                     LGEDFERKLVDAREFPAAKRLRTSNDYVEGPRAFAEKRKPNWTNT"
     gene            5654..7867
                     /locus_tag="PKV56_14450"
     CDS             5654..7867
                     /locus_tag="PKV56_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOX69062.1"
                     /translation="MSEALPLVESEDLVLVRDSARGLLQDAWPASRAVAAAKDAAALK
                     DMWRKAALQGWTALAPSQDEMGLAASIVLMQELGRAACPLPLADAVLANAVLAQAQGE
                     AAAQLLASVQSGEAIVAWAADPHGGRVTDGATLDGTVSFVEHGAIATHFLVATGKGEI
                     AIVPREAAGLAIDATPGLNVPPLSQLRFTRVDTFQRVTTSFDVGTLTPLMRLLLAARA
                     TGSAGYGLELLTDYAKVRSQFGHKIGSYQAIQHKLANCFTSVEICRLALVAAGRASQH
                     ERAYSAAVAAANAGQLLRDVVRELHHGFGGISFWDEHELPRHFRRIHGDLTRLGGVQQ
                     ARRDVAALLLERGAVPDMALTPAADAFRAEVREWLAHNWDHAYPPETYALPVNHRKAR
                     QDFSRKVGAKGWLGVNWPKAYGGQGRTAFEHLAFEEEMAYAEAPVTFHNTSANMIGPT
                     LVIHGSDAQKAFFLPGIARGDISIALGYSEPDHGSDLAGIRTSAKRTEDGGWVVNGQK
                     IFTSTAGFSQYVWLAARTDPANQRHGGISVFLVPLDTPGITIQPMSGLNGHRANVVFF
                     DDVKLPADALVGPENGGWKLITDALAFERETVGAIGARARGYFDKLLAHVKADASLRS
                     DPLVLDRIGGLGAEIEASRLLAVRTAQVVEQGEVPLWQAAMLKVYASELMERLSETAF
                     DLLGPGATLAEGAQGALCDSVFEYGVRDALLYTIGGGTNEIQRTLIALRGLDLPR"
     gene            7938..8888
                     /locus_tag="PKV56_14455"
     CDS             7938..8888
                     /locus_tag="PKV56_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOX69063.1"
                     /translation="MLAASLLALAPLAPLAHAQGFPTKPIKIVITYAPGGTGDVIART
                     LATSMSELLGQPVVVENKTGAFGSIGTMAVKQAPPDGHTLLVTTIGTVVLAPMVTKDA
                     SFDPVKGLTPIANLAMAPMVLLVHQSVPANDFPGFVTWAKQQAKGVDIAVAGPTPEVA
                     MALLAQQAKLNLVNVPFRGSGPALQSVLAGDTKVIFQTPSVQMMEFIKQGKLKVIGVS
                     SAQPSPAVPGAVPLNKFVPGYVQDINFALWAPPGTPADVAAKLTETVRKGLSAPGMAE
                     KFVAFSVPLAVAGPDEVTRITTRESNNIKKIMETVPIKFE"
     gene            9060..>9245
                     /locus_tag="PKV56_14460"
     CDS             9060..>9245
                     /locus_tag="PKV56_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOX69064.1"
                     /translation="MTMQMNRRNALAAFAAAAVPGAWAQDRYPSRPIRIIVTNAAGGA
                     SDMIARMIGTGLTAALGQ"
BASE COUNT         1516 a         3176 c         3161 g         1292 t
ORIGIN      
        1 tgccgcgacg tcgtggtcga ggtcaccaag gccggcggct tcggcgtgct gggcgcggtc
       61 acgcactcgc ccgaacagct cgagcgcgaa ctcaagtgga tcgacgagca cgtgggcggc
      121 cggccctacg gcgtggacgt gctcattccc gtcgtctacg accgcgaggc cgaagcatcg
      181 accgaggagg tgatcgacct catccccgcc gagcagaggc agttcgtcga caagctgctg
      241 gatgcggaag gcgtgccccc gctgtccgcc gacgacgcgg ccgaagcgcg ccgcgagctg
      301 atcgggcgcg ggcgcaacac cacgccgctg ggcgcgcgca ggctgatcga cgtggcgctg
      361 cgccacccgc aggtcaagct ggtggtcagc gccctgggtg cgccgccgaa ggaactggtc
      421 gacgagctgc acggccgcgg catcctcgtc ggctcgctgt gcggaaaggc cgagcacgcg
      481 aaggcgcagc gcgcggtggg cgtggacatc atcgtcgcgc agggcaccga agccggcggc
      541 cacacggggc agatcgccac catggtgctc gtgccgcagg tggtcgatgt cgccggcccc
      601 gacatcgcgg tgctcgccgc cggcggcatc agccgcggca ggcagatcgc ggcagccttt
      661 gcgctcggcg cgcagggcgt gtggaccgga acgatctggc tgggcacgcg cgagagcgag
      721 ctcacgccca tggaaaaaga ggtgcttttc aaggcgaagt ccgaagacgc cgtgcagcgc
      781 aagtggatga cggggaagac cgttcgcatg atccgcagca aggcctcgga agcgtgggaa
      841 cagcccggtg cgcccaggca cctgaagccg ccgctgcaga acatcctgta cctcggcgcg
      901 cgcatccgca tcgagaaggc gcatcgcgac gacctgtgct cgttccccgc cgggcaggtc
      961 gtcgggaaca tgaaggaaga gacctccgtg cgccaggtca tgcaggacct gatgcaggag
     1021 tacatcgaca ccgccgagcg catctctccc ttgatcaacc tctgaaagca cagatggaca
     1081 agacacgcat ccccgtcatc gtcggcaccg gccaggtcac cgacaccact tcgaagcccg
     1141 aagagggccg ttcgccgctg cagctgatgc acgacgcggt caagctcgcc gcggcggaca
     1201 gtggcgcgca ggaccagggc gccgcactgc tgcgcgggct ggactccgtc acggtgatcc
     1261 gcctgtttgc cgacacgctg ccgcgtttcg catcgccctt cggcaagctc gcgaatgcgc
     1321 cgtggtccat cgcgcagcgc gtgggcgcgg cgaacgccac ggacctcgtg tgtcccccgc
     1381 aaggcggcga ttcgctggtg atgctcgcgc gcgcgtgcga acgcatcgcg cagggagagt
     1441 cgcaggcggc gctggtcgtg ggcggcgagg cgctgcgcac cgagctcgcg gccaagcgcg
     1501 ctggtctgca gctgcagtgg ggcgaggacg cccccaccac gcccaaccag ctcacgggcg
     1561 tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggacat gtacaccaag
     1681 gccgaagaga agcacggcat gcgctcggcc atcgcaatgt acgcgctcat cgggcaggcc
     1741 ctgcgccacg ccgcggggca gacggtcgac cagtaccgcg aagccagcgc caagctcttt
     1801 gcacgcttcg ccgccgtggc cagggacaac ccgctcgcca cgcgccgcaa ggggtacagc
     1861 gccgagcaga tcgccgaggt gaacgcggag aaccccttcg tgggcttccc gtacaccaag
     1921 ctcatgacgg ccagcgcctt cgtcgaccag tcggccgcgt tcatcgtggt gtccgaagcg
     1981 cgtgccgacg agctcggcat cccgcaggac aagcgcgtgt acctgcacgg cgaagcgcat
     2041 gcgcacaaca cgtggttcgt gagcgaccgc gagcgcctcg accagtcgcc cgcgatgcgc
     2101 ctgacctcgc gcgccgctct ggcgcaggcg ggcaagacca tcgccgacgt ggatgtgttc
     2161 gacatctaca gctgcttccc gtcggtagtg cagatcgcct gcaaggagct gggcatctcg
     2221 cccgacgaca agcgcggcct caccgtcacg ggcggcctgc cgtacttcgg cggaccgggc
     2281 aacaactacg tgacgcacgc catcgccgag atggtgcagc gcgtgcgtgc gaagcccggc
     2341 gcctatggcc tcgtcatggc caacgtcggc ctcgtcacca aggagtcggt cggtctgttt
     2401 tccaccgagc ggccgaacaa gcccttcatg cgcgcggatt ccgacgccat ccagcgcgag
     2461 atcgacgcgc agaccaaggt gcgcttcacc gaggcaccgc agggtgacgc gaccatcgag
     2521 acctatgccg tgctgcacgg caagaacggt cccgaaagcg gcgtgctgtt cgggcgcctg
     2581 gctgctacgg gcgagcgctt cgtcgcgaac acgcccgccg atgcggcaac gctcgcgaag
     2641 ctggagcagg tcgaaggcat cgggctgccc ggcaccgtga cgcaggtgga cggccgcaac
     2701 gtgttcacgc cgcgcttcgg ataaacgcga tggcggcacc ggacacaggc ctcgcacgcc
     2761 tcttcaaccc gcggtccgtc gcgctcgtgg gcgcgaccga ccgctccacg tggtcgaaga
     2821 tggccttcga caacctgaag ctgctgggct tcgaggggaa ggtccatctc gtcaaccgca
     2881 gcggcggcgt cgtgcatggg cagcagtcgt tcaagacggc agtcgacatc ggcgagagcg
     2941 tcgacgtcgc gctgctcatg gtgcccaacc ccgcgatgga ggacgcactg cgcgacctgg
     3001 gcgccgcggg catccgccac gccgtggtgc tcgccggcgg cttcgcggaa accggcagcg
     3061 aaggccgcac gatgcaggag cgcatggtcg ccacggcgcg tgaactcggc atcacgctgc
     3121 tggggcccaa ctgcctgggc ttcatcaact tcacgaccgg cgcggtgtgc tggacgggcg
     3181 ccatgcgcac gccacccctg aagggcggca tcagcatcgt gtcgcaaagc ggcgcggtcg
     3241 ccaccgtgat gaagcacttc gcgcaccagc acggcgtagg catgaacgtg gtcgccgcca
     3301 ccggcaacga ggcaatgctg ggcctggccg aatgcgtgga ctacctcgtg gacgacgaga
     3361 gcacgaaggt gatcgcggtg ttcgcggaga cggtgcgtga cacgcgcctg ttccgcgccg
     3421 ccgccgagcg cgcgctcgcg aaggccaagc cgatcgtggt gctcaaggtg ggccgctccg
     3481 acatcgcgac gcaggctgcg cagtcgcaca cgggctcgct cgtgggcgac gacagcgtgt
     3541 tcgacggcgt gtgccgccag ctcgggctgg tgcgcgtgcg cagcatcgag gagctcgtgt
     3601 tcaccgcggc gctgctcgag aaaaccggcg tgctggccga aggcggggtc gcggtgctgt
     3661 cgtcctcggg cggcatgggc gagctggccg cggactatgc gcagctggaa acactgcgcc
     3721 tgcccgcctt gtcgaatgaa acgctctcgg cgctgcgcga gatccttccg ccgatggcca
     3781 cacccgccaa cccgctggac ctgacgggcg cagtcgtcaa cgattgggcg ctcttcaccc
     3841 gctgcatgga tgcgatgcag cgcgatccgg ccgtctccgt gctggtctgc gtggccgacg
     3901 tgcccacggc gaacaacaac gactgggcgc cgcttgcggt gggaacgctg caggccatcg
     3961 ggcagttcaa gccggacgga cgcgcgcgct acctcgtggt gagcaacgcg gtgaaggcgg
     4021 tgtcggacaa gagccgcgaa gaagtggaga aggcgcagat tccgtacctc gcctgcgggc
     4081 tggacatcgc gttgcgcgcg ttgcgctacg ccgcggactg gtcccggatg tcccgcagtg
     4141 cagggggcga gcgcgcggca cgcgccttgc cgggtgcggc gtcgacggca ggcgccgact
     4201 tgccgcaatc cgagcgcgaa acggtggact acctcaagcg cttcggcgtc cccgtggttc
     4261 cccaggtgct cgccaccgac gcgaagcagg ccgtcgccgc ggcgcgcgac atgaacggcc
     4321 cggtcgtcct caagatcgcc tcgccggaca tcgcgcacaa gaccgaggtc ggcggcgtcg
     4381 tgctcaacct gcagggcgac gacgcggtcg aagcggcgtt ccgcaggatc atggacgcgg
     4441 ccgcctccgc gatgccgaag gcacggctcg acggcgtgat cgtttcgccc atgcgcaagg
     4501 gcggcatcga gctcttcacg ggcgtgcgcg tcgacgccca gtggggcccc gtcatcgcgc
     4561 tcggactggg cggcgtgtgg atcgaggcgc tgcaggacgt gagcctgcgc ctcctgcccg
     4621 tgacgccagc cgacgtggaa cagatggtgt ccgagctgcg cggcgcgaag ctgctgcaag
     4681 gctttcgcgg cgcgccgccg gtcgatgtcg cgaagctcgc cgacgccgtg gcgcgcatcg
     4741 gcgacgccgc gctcgcgctc ggcgccacgc tggacacgct ggaagtcaac ccgctgctcg
     4801 ccgacggcgc gcgcatcgag gcactcgatg ccctcgccac ctacaaccac tgaaagagac
     4861 acaccatggc catcacgcaa cgcgagttct gcaccgtcga gaaagacgga cacctcacca
     4921 tcgtcacgat caaccgtccc gacgtgatga actccacgca ctacgaggcg gacctcgaac
     4981 tcgaccaggt gtgggacgag ttcgcagagg accccgagca gtgggtcggc atcatcaccg
     5041 gcgcgggcga ccgcgcgttc agcaccggca acgacctgaa gatgcacgcc aagcgcggcg
     5101 tgcggcagat tcccaagggc ggcttcgccg gcctgaccac gcgattcgac ctggacaagc
     5161 ccgtcatcgc cgcggtgaac ggcatcgcca tgggcggtgg cttcgagctc gcgctggcgt
     5221 gcgacctcgt catcgcggcg gagaacgcgt tcttcgcctt gtccgagccg cgcgtgggcc
     5281 tgtcggcact cgcggggggc gtgcagtacc tgccgcgcgc catcggcctg ccgcgcgcca
     5341 tgggcatcct gctcacgggg cgccgcgtgc cggcgaaaga gggctacgaa ctcggcttcg
     5401 tcaccgcggt ggcgccggca ggcggcgcga tggaagaggc gcgcaagtgg gccgcgcaga
     5461 tgctggagtg ctcgccgctc gcgctgcgcg ccaccaagga agtcgcgcgc aagaccatgc
     5521 tgggtgagga cttcgagcgc aagctggtgg acgcgcgcga gttcccggcg gccaagcgcc
     5581 tgcgcaccag caacgactac gtcgaaggcc cgcgcgcctt cgccgagaag cgcaagccga
     5641 actggaccaa cacatgagcg aggcccttcc cctggtggag agcgaggacc tcgtcctcgt
     5701 gcgcgattcg gcgcgcggcc tgctgcagga cgcctggccc gcatcgcgcg ccgtcgccgc
     5761 tgcgaaggac gcggccgcgc tgaaggacat gtggcgcaag gccgcgctgc agggctggac
     5821 tgcgctcgcg ccgtcgcagg acgagatggg acttgccgcg tccatcgtgc tgatgcagga
     5881 actcggccgt gcggcctgcc cgctgccgct ggcggatgcg gtgctcgcga acgcggtgct
     5941 cgcccaggcg cagggcgagg cggccgcgca actgctggca agcgtgcagt cgggcgaggc
     6001 catcgtcgcg tgggcggccg atccgcacgg cggcagggtg acggatgggg ccacactcga
     6061 tggcaccgtc tccttcgtcg agcacggcgc catcgccacg cactttctcg tcgcgaccgg
     6121 caaaggcgaa atcgcgatcg tgccgcgcga ggccgcgggg ctcgcgatcg atgccacgcc
     6181 cggcctgaac gtgccgcccc tgtcgcagct gcgcttcacg cgggtggaca cgttccagcg
     6241 cgtcaccacg tcgttcgacg tcggcacgct cacgcccctg atgcgcctgc tgcttgcagc
     6301 acgcgccacg ggttcggcgg gctatggcct ggagctgctg accgactacg ccaaggtgcg
     6361 ctcgcagttc ggccacaaga tcggcagcta ccaggccatc cagcacaagc tcgcgaactg
     6421 cttcaccagc gtggagatct gccggctcgc gctcgtcgcg gcgggccgtg catcgcagca
     6481 cgagcgcgcc tacagcgccg ccgtcgcggc cgcgaatgcg ggccagctgc tgcgcgacgt
     6541 ggtgcgcgaa ctgcatcacg gcttcggcgg catttcgttc tgggacgagc acgagctgcc
     6601 gcgccacttc cgccgcatcc acggcgacct cacgcggctc ggtggcgtgc agcaggcccg
     6661 gcgcgacgtc gcggccctgc tgctcgaacg cggcgccgtc cccgacatgg cgctcacgcc
     6721 ggcggccgat gccttccgcg ccgaggtgcg cgagtggctg gcgcacaact gggaccatgc
     6781 ctatccgccc gaaacgtatg cgctcccggt gaatcaccgc aaggcgcgtc aggacttcag
     6841 ccgcaaggtg ggcgccaagg gctggctcgg cgtcaactgg cccaaggcct acggcggaca
     6901 gggacgcacg gccttcgaac atctcgcctt cgaggaggag atggcctacg cggaggcgcc
     6961 cgtcaccttc cacaacacct cggccaacat gatcggcccg acgctcgtca tccacggcag
     7021 cgacgcgcag aaggcgttct tcctgcccgg catcgcgcgc ggcgacatct cgatcgccct
     7081 cggctacagc gagccggacc acggctccga cctcgcgggc atcagaacat ccgcgaagcg
     7141 caccgaggac ggcggctggg tggtgaacgg tcagaagata ttcacgtcga ccgccgggtt
     7201 ctcgcaatac gtgtggctgg ccgcgcgcac cgacccggcc aaccagcggc acggcggcat
     7261 cagcgtcttc cttgtgccgc tggacacgcc cggcatcacg atccagccca tgagcggcct
     7321 caacggccac cgcgcgaacg tggtgttctt cgatgacgtg aagctgcccg ccgacgcgct
     7381 cgtcgggccg gagaacggcg gctggaagct catcaccgat gcactcgcct tcgagcgcga
     7441 gacggtgggc gcgatcggcg cacgtgcgcg gggttacttc gacaagctgc tcgcgcacgt
     7501 gaaggccgac gcttcgctgc gcagcgaccc gctggtgctc gaccgcatcg gcgggctggg
     7561 cgcggagatc gaggcgtcgc gcctgctcgc cgtgcgcacc gcgcaggtgg tggagcaggg
     7621 cgaggtgccg ctgtggcagg ccgccatgct gaaggtgtac gcgagcgagc tgatggagcg
     7681 cctgtccgag acggcgttcg acctgctcgg ccccggcgcc acgctcgccg agggcgcgca
     7741 aggcgcgctg tgcgacagcg tgttcgagta cggcgtgcgc gatgcgctgc tttacacgat
     7801 cggtggcggc accaacgaga tccagcgcac cctgatcgcg ctgcgagggc tggacctgcc
     7861 tcgttgatcc ggctgtttca ttccaactag atcctcatcc tggagactct ctcatgcaag
     7921 gcttccgacg ttccctcttg ctggcggcat cgctgctggc gctcgcgccc ctcgcgcctc
     7981 tcgcccacgc gcaaggcttc cccacgaagc cgatcaagat cgtcatcacg tacgcgcccg
     8041 gcggcacggg tgacgtgatc gcgcgcacgc ttgccacgtc gatgagcgag ctgctcggcc
     8101 agccggtggt ggtggaaaac aagaccggcg ctttcggctc catcggcacc atggccgtga
     8161 agcaggcacc ccctgacggc catacgctgc tggtgacgac catcggcacc gtcgtgctgg
     8221 cgccgatggt gaccaaggac gcgagcttcg acccggtcaa gggcctcacg ccgatcgcca
     8281 acctcgcaat ggcgccaatg gttctgctcg tgcaccagag cgttcccgcc aacgacttcc
     8341 cgggcttcgt cacgtgggcg aagcagcagg ccaagggcgt ggacatcgcc gtcgccggcc
     8401 ctacgccgga ggtggcgatg gcgctgctcg cgcagcaggc caagctcaat ctcgtgaacg
     8461 tgcctttccg cggcagcggc cccgcgctgc agtcggtgct ggccggcgac accaaggtca
     8521 tcttccagac gccgtcggtg cagatgatgg agttcatcaa gcagggcaag ctcaaggtga
     8581 tcggcgtgtc gagcgcgcag cccagccccg cggtgcctgg cgcggtgccg ctcaacaaat
     8641 tcgtgccggg ctatgtgcag gacatcaact tcgcgctgtg ggcgccgccc ggcacgccgg
     8701 ccgacgtggc cgccaagctg accgagaccg tgcgcaaggg cctgtcggca ccgggcatgg
     8761 ccgagaagtt cgtcgcgttc tccgtgccgc ttgcagtggc cgggcccgat gaagtgaccc
     8821 gcatcaccac gcgcgagtcg aacaacatca agaagatcat ggaaaccgtg ccgatcaagt
     8881 tcgagtgagg tcgacccacc cccgaagcgc ctgcggcgct accccctcaa ggggtcgccc
     8941 ccagcggacc ggcaaagccg gatccgcggc ggcactggaa acacgtaacg aaagcctcct
     9001 atgaacgtgc gtacctttct caagaccatc atggaaacga caaccgtcaa gttcggggaa
     9061 tgacgatgca gatgaatcga cgcaacgccc tggccgcctt cgcggcggct gccgttcctg
     9121 gcgcatgggc gcaggaccgc tatccgagcc gccccatccg catcatcgtg acgaacgccg
     9181 ccggcggcgc atcggacatg atcgcgcgca tgatcggcac gggcctgacc gctgcactgg
     9241 ggcag
//
LOCUS       DAOORV010000132         9189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9339_length_9189_cov_7.909350, whole genome shotgun sequence.
ACCESSION   DAOORV010000132 DAOORV010000000
VERSION     DAOORV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_9189_cov_7.909350"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PKV56_14465"
     CDS             <1..1173
                     /locus_tag="PKV56_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOX69065.1"
                     /translation="ALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGL
                     VAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIKAGQLTVEKKGKKN
                     IFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVHLNKEPIIEYIHSNITLMKWM
                     ISEGYADARTLARRIAAQEAWLADPQLLKGDADAEYAAVIEIDLADIHEPIVACPNDP
                     DDVKTLSEVAGAKIDEVFIGSCMTNIGHFRAASKLLENKRDIPVKLWVAPPTKMDAKK
                     LSDEGHYGVLGSAGARMEMPGCSLCMGNQAQVKEGATVFSTSTRNFPNRLGKNSNVYL
                     GSAELAAICSKLGRIPTREEYLQDMGVLTAASDKVYQYLNFDKIEEFTDAAEKASA"
     gene            1302..2090
                     /gene="fabI"
                     /locus_tag="PKV56_14470"
     CDS             1302..2090
                     /gene="fabI"
                     /locus_tag="PKV56_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOX69066.1"
                     /translation="MGFLTGKRLLITGVLSSRSIAYGIARACHREGAELAFSYVGERF
                     KERITELAAEFGSTMVYDCDVADDAQIERLFSDMGELWPEFDGFVHSIGFATRESIAG
                     DFLDGLSREGFRIAHDISAYSFPAMAKAALPRLRPGAALLTLTYLGAARVVPNYNTMG
                     LAKASLEASVRYLASSLGPRGIRVNGISAGPIKTLAASGIKGFGKILEVVETNAPLRR
                     NVTIDDVGNVAAFMLSDLAAGVTSEITYVDAGFSHAMGGGTDAG"
     gene            complement(2091..2714)
                     /locus_tag="PKV56_14475"
     CDS             complement(2091..2714)
                     /locus_tag="PKV56_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOX69067.1"
                     /translation="MIVQQPVTTFLDDLASGSATPGGGSAAAIMGAMGAALVSMMCNL
                     TIGKKNYAEVEGEMQSLLAAAEELRLQLAGMVAEDIGAFDALMAAYAMPKDTDEQKTK
                     RSAAIQEGLKAATDVPLACARASAGVIAVAMRAAQVGNRNVISDAGVGALAAQAALRS
                     AALNVYINVPSIRDEAFAFSRRTEIDALLASAVPLAEQVYELVKNEL"
     gene            complement(2731..3594)
                     /locus_tag="PKV56_14480"
     CDS             complement(2731..3594)
                     /locus_tag="PKV56_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HOX69068.1"
                     /translation="MKKLLFQIDTDPIPAVFDTVVAYDGGADHVTAHGGITPDNVGPL
                     VDGTIFTRSPREKRFTAIFVGGSNLAAGEALLKAVQKKFFSSFRVSVMLDSNGSNTTA
                     AAAVACLLKSAGGSLAGQRAVILAGTGPVGQRAAVMLAKEGVSVAITSRTLGNAQKAC
                     DHLKAGFGIDVTAIEAPDLDSRARACEGAQVVLATGAASSVLLRESDWKSNPTIRYMA
                     DANATPPLGIEGMDMMDRGIERDGRICWGAIGFGAVKLAVHRACIAQLFEAPDQVLDA
                     EAIYAQAKSMV"
     gene            complement(3642..4592)
                     /locus_tag="PKV56_14485"
     CDS             complement(3642..4592)
                     /locus_tag="PKV56_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOX69069.1"
                     /translation="MTEHLVFLDRSTLQATVRRPGFDHTWEEFPASSAEQAVERLRHA
                     TIALTNKVPLRAEALARLPKLKLIAVAATGYDVIDVAACRERGIAVCNIRNYAVHTVP
                     EHVFALILALRRSLLAYRADVERGRWQQADQFCFFDHPIRDLHGSTMGIFGGGTLGQG
                     TAALARAFGMRVLFAEVSPTGKAGAERSPVAQVLAESDVISMHCPLTPATRNLIGVEQ
                     LRRMKPSAILINTARGGLVDEEALVTALKEGWIAGAGFDVLTTEPPRLGNPLLDLRLP
                     NFILTPHVAWASGGAMQILADQLIDNVELFVKGTPRHLVS"
     gene            4700..5677
                     /locus_tag="PKV56_14490"
     CDS             4700..5677
                     /locus_tag="PKV56_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOX69070.1"
                     /translation="MSFTVIPQASPRLHRSELAVPGSKTHLFEKAARSAADAVFLDCE
                     DAVAPDDKEQARRNIVAALNDIDWGSKTVMVRINGLDTHYMYRDVIDIVEACPRLDMI
                     LIPKVGVAADIYAVDMLVSQIEAAKQRTKRIGFEALIETALGMANVEAIAQSSKRLEA
                     MSFGIADFAASTRARTTSIGGVHPDSTVLSDPDAAGQRHAFWTDPWHAAQARMLVACR
                     AYGLRPVDGPFGNFADPEGFVSAARRAAVLGYEGKWVIHPSQIEPANEIYTPTEAELH
                     QARRIIEALAHANKQGLGAVSLDGRLIDVVNIRMAQALMAKAAAIGAPR"
     gene            5792..7465
                     /locus_tag="PKV56_14495"
     CDS             5792..7465
                     /locus_tag="PKV56_14495"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOX69071.1"
                     /translation="MASDIDIAQQATMHRINQVAARIGIPEDALEPYGHYKAKISLEY
                     LNSLDVRPDGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKTMICLREPSLGPVF
                     GVKGGAAGGGYAQIVPMEDINLHFTGDFHAIGAAHNLLSALIDNHIHQGNELRIDPRL
                     IQWKRVVDMNDRALRKIVVGMGGTANGFVREDGYDIVVASEVMAILCLALSRNDLKAR
                     LGRIVIGYTQGDRKPVYARDLNAQGAMATLLKDAIKPNLVQTLENNPAIIHGGPFANI
                     AHGCNSVMATKAGLKLADYVVTEAGFGADLGAEKFIDIKCRMAGLKPAAVVLVATLRA
                     LKFHGGVKKEDLNRENLEALDKGIVNIERHLKNITLHYGLPCVVSVNHFTFDTEAEIA
                     LLKGRIEALGGHFVLARHWAEGGKGAEDLARAVVALAENAHGPHKFVYEDTDTLQAKI
                     TAVATKIYGAGQVTFTDAVRRQLDEWNKDYGNFPVCMAKTQMSFSADPSVRGAPSGHT
                     LNVREVRLANGAGFIVAICGDMMTMPGLPKVPSAERIDIDDDGKVSGLF"
     gene            complement(7503..7922)
                     /locus_tag="PKV56_14500"
     CDS             complement(7503..7922)
                     /locus_tag="PKV56_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOX69072.1"
                     /translation="MFTYICLGTSDLVRAAAFYDPVMAALGLQRCDTSNEPDWEGWVG
                     WGTYLDEGRVEVALWLCKPFNGEPASVGNGTMVAFAAASWEQVQAFHREALQHGGISE
                     GEPGLRVHYGPDFYAAYVRDPDGHKLAAVCRGIEPPA"
     gene            complement(7945..>9189)
                     /locus_tag="PKV56_14505"
     CDS             complement(7945..>9189)
                     /locus_tag="PKV56_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HOX69073.1"
                     /translation="YNTETIKHALDGYIDTLHFDEAWLPHAAFHRFYGSYHAMGKKRV
                     RPKDQIVFATQSTHKLLAGISQASQVLVQDAVDRKLDRHLFNEAYLMHTSTSPQYAII
                     ASCDVAAAMMEAPGGPALVEESISEALDFRRAMRKVDKEFGRDWWFKVWGPDKLCADG
                     IGKSADWVLKAGEKWHGFGDLAEGFNLLDPIKSTVITPGLDMSGKFAKTGIPASIVTK
                     YLAEHGIVVEKTGLYSFFIMFTIGITKGRWNTMVTALQQFKDDYDRNLPMWKMLPEFV
                     AKYPRYERMGLRDLCQSIHAMYAKGDIARLTTEMYLSDLQPAMKPSDAYAYIAHRRTE
                     RVEIESLEGRITTTLLTPYPPGIPLLIPGEVFNKKIVDYLRFTRDFNAAFPGFDTDVH
                     GLVEEDLEGGGKRYGVDCVRED"
BASE COUNT         1636 a         3043 c         2998 g         1512 t
ORIGIN      
        1 gcgctgcgcc cgggcgacgg cgtgatccac agctggctca accgcctgct gctgcctgac
       61 acggtgggca ccggcggcga cagccacacg cgcttcccga tcggcatcag cttccctgcc
      121 ggttcgggcc tggtggcgtt cgccgccgcc accggcgtga tgccgctcga catgcccgag
      181 agcgtgctgg tgcgcttcaa gggcaagatg caacccggcg tgacgctgcg cgatctggtc
      241 aatgcgatcc cgctgtacgc gatcaaggcc ggtcagctga ccgtcgagaa gaagggcaag
      301 aagaacatct tcagcggtcg catcctcgaa attgagggct tgcccgacct gaaggtggaa
      361 caggccttcg aactgagcga tgcttcggcc gaacgcagcg ccgccggttg caccgtgcac
      421 ctgaacaagg agccgatcat cgagtacatc cacagcaaca tcacgctgat gaagtggatg
      481 atctccgagg gctacgccga tgcccgcacg ctggcgcgcc gcatcgcagc gcaggaggcc
      541 tggctggccg atccgcaatt gctgaagggc gacgccgatg ccgaatacgc cgcggtgatc
      601 gagatcgacc tggccgacat ccacgagccg atcgttgcct gcccgaacga cccggacgac
      661 gtgaagacgc tctccgaggt tgccggcgcg aagatcgatg aagtgttcat cggctcgtgc
      721 atgaccaaca tcggtcactt ccgcgccgcc agcaagctgc tcgagaacaa gcgcgacatc
      781 ccggtcaagc tgtgggttgc accgccgacc aagatggacg cgaagaagct cagcgacgaa
      841 gggcactacg gcgtgctcgg aagcgccggc gcgcgcatgg aaatgccggg ctgcagcctg
      901 tgcatgggca accaggcgca ggtcaaggaa ggcgcgaccg tgttctcgac gagcacccgc
      961 aacttcccga accgcctcgg caagaacagc aacgtctatc ttggctcggc agaactggcc
     1021 gcgatctgct cgaagctcgg ccgcatccct acccgcgagg agtacctgca ggacatgggc
     1081 gtgctgaccg cagccagcga caaggtctac cagtacctga acttcgacaa gatcgaggag
     1141 ttcacggacg ccgcggagaa ggcatccgcc tgattcccgc cgctggcgac agccccatca
     1201 agccccgctg ctcgcagcgg ggctttttgt tgggacagcg caggtaggca gccgcggact
     1261 gcatgcaaaa tcgcgcggct tcgattgttc aaggagaaag catgggattc ctgactggca
     1321 agcggctctt gatcactggc gtcctgtcca gccggtcgat cgcctacggc atcgcgcgcg
     1381 cttgccaccg agaaggcgcc gaactggcgt tcagctacgt cggcgaacgc ttcaaggagc
     1441 gcatcaccga actggcggcc gaattcgggt cgacgatggt ctacgactgc gatgtcgccg
     1501 acgatgcgca gatcgagcgg ttgttcagcg acatgggcga gctgtggcct gaattcgacg
     1561 gcttcgtgca ctcgatcggc tttgccacgc gcgagtcaat cgccggcgac ttcctcgacg
     1621 gcctgtcgcg cgaggggttt cgcatcgcac acgacatctc ggcctacagc ttcccggcca
     1681 tggccaaggc ggccctgccc cggctgcgcc ccggtgccgc cttgctcacg ctgacctacc
     1741 tcggcgcggc gcgcgtcgtg ccgaactaca acacgatggg cctggccaag gcctcgctcg
     1801 aagccagcgt gcgctacctc gcgtccagcc tcgggccgcg cggcatccgc gtcaacggca
     1861 tctcggccgg gccgatcaag acgctggccg cgtcgggcat caagggcttc ggcaagatcc
     1921 tggaagtggt cgagaccaat gcgccactgc gccggaacgt gacgatcgac gacgtcggca
     1981 acgtggccgc cttcatgctt tccgacctgg cagccggcgt gacctccgag atcacgtacg
     2041 tcgacgccgg attcagccac gcgatgggcg gcggcaccga cgccggctga tcagagctcg
     2101 ttcttgacca gttcgtagac ctgctctgcc agcggcaccg cgctggcgag cagcgcatcg
     2161 atctcggtac gccgcgagaa cgcaaacgcc tcgtccctga tgctaggcac gttgatatag
     2221 acgttcagcg cagcgctgcg cagcgctgcc tgcgcagcga gcgcgccgac cccggcgtcg
     2281 ctgatgacgt tgcgattgcc cacctgtgcc gcacgcatgg ccaccgcaat cacgccggcg
     2341 ctggcacgcg cgcaggccag cggcacgtcg gtggccgcct tcagcccttc ctggatggca
     2401 gcactgcgct tggtcttttg ctcatcggtg tccttgggca tcgcgtaggc ggccatcagt
     2461 gcatcgaacg caccgatgtc ttcggccacc atgccggcga gctgcaggcg aagttcctcg
     2521 gcagctgcga gcagcgactg catctcgccc tcgacctcgg cgtagttctt cttgccgatg
     2581 gtcaggttgc acatcatgct gaccagggcc gcacccatcg cgcccatgat ggccgcggcg
     2641 ctgccaccac cgggtgtggc cgagccgctg gcaaggtcgt ctagaaaggt cgtgacgggt
     2701 tgttgaacga tcatggtggc ggcgttccgg tcagaccatc gatttggctt gggcgtagat
     2761 ggcctcggcg tccagcacct ggtcgggcgc ctcgaacaac tgcgcgatgc aggcacgatg
     2821 cacggcgagc ttgaccgcgc cgaatccgat cgctccccag cagatgcggc cgtcacgctc
     2881 gatgccgcga tccatcatgt ccatgccctc gatgccaagc ggcggcgtgg cgttggcgtc
     2941 ggccatgtag cggatggttg ggttgctttt ccagtccgac tcgcgcagca gaaccgagct
     3001 cgccgcgccg gtggccagaa ccacctgtgc gccctcgcag gcccgagcgc gggaatccag
     3061 atcgggcgcc tcgatcgcgg tcacgtcgat accgaagccg gccttcaggt gatcgcacgc
     3121 cttctgcgca ttgcccaggg tccgcgaggt gatcgccacc gagacgcctt ccttggccag
     3181 catcaccgcc gcacgctgac cgaccgggcc ggtgccggcg aggatcaccg cccgctgccc
     3241 ggccagactg ccgccggcac tcttcagcag acaggccacc gccgcagcgg ccgtcgtgtt
     3301 cgagccattg ctgtcgagca tcaccgagac gcggaaactg gagaagaact tcttctggac
     3361 ggccttcagc agggcctcgc ccgcagccag gttgctgccg ccgacgaaga tcgcggtgaa
     3421 gcgcttctcc ctcggtgagc gcgtgaagat cgttccgtcg accagcgggc cgacgttgtc
     3481 gggcgtgatg ccgccgtggg ccgtcacgtg atcggcaccg ccgtcgtagg ccaccacggt
     3541 atcgaacacc gcgggaatcg ggtcggtgtc gatctggaac aggagcttct tcatgtcgtg
     3601 atctctaaca agtggatgcg atgcatgcgc ggaacgaacc gtcaggacac cagatgccgc
     3661 ggggtgcctt tgacgaacaa ttcgacattg tcgatcagct gatcggccag tatctgcatg
     3721 gcaccaccgg aagcccaggc gacgtgcggc gtcaggatga aatttggcag ccgcaggtcg
     3781 agcagggggt tgcccaggcg gggcggttcg gtggtgagca catcgaagcc ggcgccggcg
     3841 atccagccct ccttcagggc cgtgaccagc gcctcttcgt cgaccagccc gccgcgcgcg
     3901 gtgttgatca ggatcgcgct gggcttcatc cggcgcaact gctcgacacc gatcaggttg
     3961 cgggtggctg gcgtgagcgg gcaatgcatc gagatcacgt cggattcggc cagcacctga
     4021 gcgaccggcg agcgctccgc accggccttg cctgtcggcg agacttcggc gaacagcact
     4081 cgcatcccga acgcgcgggc cagtgcggcg gtgccctgcc ccaacgtgcc gccgccgaat
     4141 atgcccatcg tgctgccgtg aaggtcgcgg atcgggtgat cgaagaagca gaactgatcg
     4201 gcctgctgcc agcgaccgcg ctccacatcc gcgcggtaag ccagcaggct gcgccgcagc
     4261 gccaggatca gggcaaaaac gtgctcgggc accgtgtgaa ccgcgtaatt ccggatgttg
     4321 cagaccgcga tgcctcgttc gcggcaagcg gcgacatcga tcacgtcgta gcccgtggct
     4381 gcaaccgcga tcagcttcag cttcggcaac cgggccagcg cctcggcgcg cagtggcacc
     4441 ttgttggtca gcgcaatggt ggcatggcgc aggcgctcga ccgcctgctc ggccgatgag
     4501 gcgggaaact cttcccaggt gtggtcgaag ccaggacgac gcacggtcgc ctgcaaggtc
     4561 gatcggtcga gaaacaccaa gtgttcggtc atgtcgtggt cctgtcgggc ggtttggtca
     4621 tcgaatgatc gggcgtatcg tgctgcgcgg tcgatcaaaa tcccgcattg tcgacagagc
     4681 ttgccgccag gagtccccca tgagcttcac cgtcattccg caggccagcc cacgtctgca
     4741 ccgatcggag ctggcggtcc cgggctcgaa gacccacctc ttcgagaaag cagcgcgctc
     4801 cgctgccgac gcggtgttcc tggactgcga agacgccgtg gcgcccgacg acaaggaaca
     4861 ggcgcgccgg aacatcgttg ccgccctcaa cgacatcgac tggggcagca agaccgtgat
     4921 ggtgcgaatc aacgggctcg acacgcatta catgtaccgc gacgtgatcg acatcgtcga
     4981 agcctgccca cggctggaca tgatcctgat cccgaaggtc ggcgtcgctg ccgacatcta
     5041 cgcggtggac atgctggtct cgcagatcga ggctgcgaag cagcgcacca agcgcatcgg
     5101 cttcgaggcg ctgatcgaga cggcgctcgg catggcgaac gtggaggcca tcgcgcaatc
     5161 gtcgaagcgg ctggaggcca tgagcttcgg catcgccgat ttcgccgcgt cgacgcgcgc
     5221 acgcaccacc tccatcggtg gcgtgcatcc ggacagcacc gtgctcagcg accccgatgc
     5281 cgcaggccag cgccacgcct tctggaccga tccgtggcac gccgcgcagg cccgcatgct
     5341 ggtggcctgc cgcgcctacg gcctgcgacc ggtcgacggg cctttcggca attttgccga
     5401 tcccgaaggc ttcgtgtccg cagcgcggcg cgccgcggtc ctcggctacg aaggcaagtg
     5461 ggtgatccac ccgtcgcaga tcgagccggc caacgagatc tacacaccga ccgaagccga
     5521 actgcatcag gcgcggcgca tcatcgaggc actggcgcat gccaacaagc agggactggg
     5581 cgcggtatcg ctcgatggcc ggttgatcga cgtcgtgaac atccgcatgg cccaggcgct
     5641 gatggccaag gccgccgcga taggcgcccc acgctgaaag ccgggcacga aagtaccgag
     5701 tccgcggcgg cggctcccgt ataaagttgc gccccagcgc atcgagggcc acaagccgga
     5761 gttctcgagc accacgcatc acggagtaga catggcatcc gatatcgaca tcgcccagca
     5821 ggccacgatg caccgcatca accaggtggc ggcccgcatc ggcatcccgg aggatgcgct
     5881 cgagccctat gggcactaca aggcgaagat ctcgctcgag tacctgaaca gcctggatgt
     5941 gcgccccgat ggcaagctga tcctggtcac cgcgatcagc ccgacgcccg ctggcgaagg
     6001 caagaccacc accacggtgg gcctgggcga tgcgctgaac cgcatcggca agaagacgat
     6061 gatctgcctg cgcgaaccgt cgctcggccc ggtgtttgga gtcaagggcg gcgcagccgg
     6121 cggcggatac gcgcagatcg tgccgatgga agacatcaat ctgcatttca cgggtgactt
     6181 ccacgcgata ggcgccgcgc acaacttgct gtcggcgctg atcgacaacc acatccacca
     6241 gggcaacgag ctgcgcatcg atccgcgcct gatccagtgg aagcgcgtcg tcgacatgaa
     6301 cgatcgcgcg ctgcgcaaga tcgtcgtcgg catgggcggc acggccaatg gcttcgtgcg
     6361 cgaggacggc tacgacatcg tcgtggcctc ggaagtgatg gccatcctgt gcctggcgct
     6421 ctcgcgcaac gacctgaagg cgcgcctggg ccgcatcgtc atcggctaca cccaaggtga
     6481 ccgcaagccg gtctacgccc gcgacctgaa tgcccagggc gcgatggcca ccctgcttaa
     6541 ggatgcgatc aagccgaacc tggtgcagac gctcgagaac aacccggcca tcatccacgg
     6601 cggcccgttc gccaacatcg cgcatggctg caactcggtc atggccacga aggccggcct
     6661 caagctggcc gactacgtgg tcaccgaggc aggcttcggc gccgacctcg gtgccgaaaa
     6721 gttcatcgac atcaagtgcc gcatggccgg gctgaagcct gccgcggtgg tgctggtggc
     6781 gacgctgcgg gcgctgaagt tccacggcgg cgtcaagaag gaagacctga accgggagaa
     6841 cctcgaagcc ctcgacaagg gcatcgtcaa catcgagcgc cacctgaaga acatcacgct
     6901 gcactacggc ttgccgtgcg tggtgtcggt caatcacttc accttcgaca ccgaagccga
     6961 gatcgcgctg ctgaaggggc gcatcgaggc gctgggcggt catttcgtcc tggcgcgtca
     7021 ctgggccgag ggtggcaagg gggccgagga cctggcgcgc gcggtggtcg cgcttgccga
     7081 gaacgcgcat ggccctcaca agttcgtcta cgaggacacc gacacgctgc aggccaagat
     7141 caccgccgtc gccacgaaga tctacggtgc agggcaggtg accttcaccg atgcggtgcg
     7201 tcggcagctc gacgagtgga acaaggatta cggcaacttc cccgtctgca tggcgaagac
     7261 gcagatgtcg ttctcggccg acccctcggt gcgcggcgcc ccgagcggcc acacgctgaa
     7321 cgtgcgcgag gtgcggctgg ccaacggcgc cggcttcatc gtcgcgatct gcggcgacat
     7381 gatgaccatg ccgggtctgc cgaaggtgcc ttcggcagag cggatcgaca tcgacgacga
     7441 cgggaaggtg tcgggtctgt tctgaagctc ggcaggcggc aagcccgttc ccgccagcga
     7501 aatcaggccg gcggctcgat gccgcggcac accgccgcca gcttgtggcc gtccgggtcg
     7561 cgcacgtagg ccgcgtagaa gtcggggcca tagtgaacgc gcagaccggg ctcgccttcg
     7621 gagatgccac cgtgttgcag ggcctcacgg tggaaggcct gcacctgctc ccatgacgca
     7681 gcggcaaaag ccaccatggt cccgttgccg acgcttgcag gctcgccgtt gaaaggcttg
     7741 cacagccaca gcgcgacctc gacacgtcct tcgtcgaggt aggtgcccca cccgacccag
     7801 ccctcccagt cgggctcgtt cgatgtgtcg cagcgctgca ggccgagggc ggccatcacg
     7861 gggtcgtaga acgcagccgc ccgcaccagg tcactggtgc cgagacagat gtaggtgaac
     7921 attcgaagcc ccggcgcgac gccttcagtc ttcgcgcacg cagtcgacgc cgtagcgctt
     7981 gccgccgcct tcgaggtctt cctcgaccag gccgtgcaca tcggtgtcga agcccgggaa
     8041 cgccgcgttg aagtcgcgcg tgaaccgcag gtagtcgacg atcttcttgt tgaacacctc
     8101 gcccggaatc agcaagggaa tgcccggcgg gtaaggcgtc agcagcgtcg tcgtgatgcg
     8161 cccttcgagg gactcgatct cgacgcgttc ggtcctgcga tgcgcgatgt aggcgtaggc
     8221 gtcgctcggc ttcatcgccg gctgcaggtc ggagaggtac atctcggtgg tcagtcgcgc
     8281 gatgtcgccc ttggcataca tcgcgtggat gctctggcac aggtcacgca ggcccatgcg
     8341 ttcgtagcgg gggtacttgg cgacgaactc cggcagcatc ttccacatcg gcaggttgcg
     8401 gtcgtagtcg tccttgaact gctgcagcgc agtcaccatc gtgttccagc ggcccttggt
     8461 gatgccgatc gtgaacatga tgaagaacga gtagagaccg gtcttctcga ccacgatgcc
     8521 gtgctcggcg aggtacttgg tgacgatgct cgccgggatg ccggtcttgg cgaacttgcc
     8581 cgacatgtcg agccccggag tgatgacggt gctcttgatc gggtcgagca ggttgaaacc
     8641 ctcggccaga tcgccgaagc cgtgccactt ctcaccggcc ttcagcaccc agtcggcgct
     8701 cttgccgatg ccatcggcgc acagcttgtc cgggccccag accttgaacc accagtcgcg
     8761 gccgaactcc ttgtcgacct tgcgcatcgc gcggcggaag tcgagcgcct cgctgatgct
     8821 ttcttccacc agtgcgggcc cgcccggcgc ttccatcatc gccgccgcca cgtcgcagct
     8881 ggcgatgatc gcgtactgcg ggctggtgct ggtgtgcatc aggtaggcct cgttgaagag
     8941 gtggcggtcg agcttgcggt cgaccgcgtc ctgcaccagc acctgagaag cctggctgat
     9001 gccggcgagc agcttgtgcg tcgactgcgt cgcgaacacg atctggtcct tcggccgcac
     9061 gcgcttcttg cccatcgcgt ggtagctgcc gtagaagcgg tggaacgccg cgtgcggcag
     9121 ccaggcctcg tcgaaatgca gcgtgtcgat gtagccgtcg agcgcgtgct tgatggtctc
     9181 ggtgttgta
//
LOCUS       DAOORV010000133         9085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9451_length_9085_cov_7.930565, whole genome shotgun sequence.
ACCESSION   DAOORV010000133 DAOORV010000000
VERSION     DAOORV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_9085_cov_7.930565"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..564
                     /locus_tag="PKV56_14510"
     CDS             22..564
                     /locus_tag="PKV56_14510"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HOX69074.1"
                     /translation="MPPFDGFDLESFWEESEHATKEYVAATPSPAGLAKIEEQLGVKL
                     PAAYVALAQRQNGGIPRRTAHRTTERTSWAEDHIAISGIYSIGSAKPCSLCGQFGSSF
                     WVSEWGYPDIGVYFADCPSAGHDMLCLDYRACGPLGEPSVVHVDQELDYKVTRVAPTF
                     EAFVRGLEPDESFNAAQLKR"
     gene            655..891
                     /locus_tag="PKV56_14515"
     CDS             655..891
                     /locus_tag="PKV56_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69075.1"
                     /translation="MIPGITNEVIHQQHSTSSRLSAPNGTLVHAMPRTGNSAQIPVDP
                     LVHHWSVTTHIRDVTPQEISQAVAQMDFGAPKCG"
     gene            932..>1119
                     /locus_tag="PKV56_14520"
     CDS             932..>1119
                     /locus_tag="PKV56_14520"
                     /inference="COORDINATES: protein motif:HMM:NF022438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2805 domain-containing protein"
                     /protein_id="HOX69076.1"
                     /translation="MAKPVPRLTPDEIERIIATAWDDRPPFAAVLRQHGLSPGQVVQL
                     LKRELTPNAFKIWKARTQG"
     assembly_gap    1120..1219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1220..1635)
                     /locus_tag="PKV56_14525"
     CDS             complement(<1220..1635)
                     /locus_tag="PKV56_14525"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX69077.1"
                     /translation="MTEKTATVHWEGQGQNGLGQISTETGALKSHPYGFASRFEDDRL
                     GTNPEELLGAAHAACFTMAFSFACAKAGIATTSIDTTARVNLVKQGEGFVIDRIALTM
                     QATVPGLDSARFQEIAAAAKRECPLSKALASVPEITL"
     gene            complement(1660..1803)
                     /locus_tag="PKV56_14530"
     CDS             complement(1660..1803)
                     /locus_tag="PKV56_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69078.1"
                     /translation="MTPSPRQPIDDPDPYEPAIPPLEPDQGPIHPVIPDDPEHDRVVD
                     PED"
     gene            1966..2946
                     /locus_tag="PKV56_14535"
     CDS             1966..2946
                     /locus_tag="PKV56_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69079.1"
                     /translation="MSAHPLTHHEILGLIGPFTRRGRHLDLPASDRLARRLVFKPIEH
                     ADPAIASSTLIEGLQLEQPEDDTYRLTRTLALPCGLQATLQTDGTDPAELLARIEAIP
                     LQRQFQRGAGYAVALCHRLDATKGGPAAALMVLTRATARIEGLRLAMKVPAVTGIPAD
                     IELEAAHGDSVDLPQDLLAVLGWSWARLVRVGKAWTCSLRLRGSGPQRSRDAEAKLER
                     TAQHLAQTLSEPPPLFHDRRVAARWGVVVRRSIPLLVTVSLIGLAAAVPSMGLSENSV
                     LRMVIFQSPPLLLGLVFCMRELPQIEIPPWPRRPSQSAWGPQRAQASVPR"
     gene            2980..4080
                     /locus_tag="PKV56_14540"
     CDS             2980..4080
                     /locus_tag="PKV56_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69080.1"
                     /translation="MSGNPMNIPPISIAAAKAALIEQYADATLRRRATMLWGTRGVGK
                     SSVVRQVADHFKVPLVDLRLTTIEPVDIRGAIYADEVQGKTVWFPPEFLPTPDQPAGI
                     LFLDELTAADQRLQISAYSLILDRRVGNYRLPDGWQVIAAGNASFHGAVSHDMGTALA
                     DRMFHFNVQTVIDAFLAHALACGFAPEVMAYLKVRPDRLDDTPSQLANDHLVGASPRG
                     WEDISNVLKSGLSDSAKRVFVQGRIGAANAAEFFGVLREIQAAVDVVRLLASRPGDET
                     AALLPKTLDGLYGMLYGLLAACVDEKTVARALEIIEQLPDARASVPLPVRESQTLAME
                     LLMQKALEGGLEAAILDSPVYRRYAEQRSRDG"
     gene            4073..4933
                     /locus_tag="PKV56_14545"
                     /pseudo
     CDS             4073..4933
                     /locus_tag="PKV56_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4925..5344
                     /locus_tag="PKV56_14550"
     CDS             4925..5344
                     /locus_tag="PKV56_14550"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOX69081.1"
                     /translation="MPWEPGFSSSKSVSRLVVIVDVSGSIDPALMERFAREIEAITRR
                     LEAGLVLVIGDERVQRVEHFQPGRAKLSDIPFKGGGGTDFTPLLEEADTHRPDIAVVL
                     TDLDGPARFRPRWPVIWAVPQANADALAPFGRKLVLN"
     gene            complement(5352..6227)
                     /locus_tag="PKV56_14555"
     CDS             complement(5352..6227)
                     /locus_tag="PKV56_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69082.1"
                     /translation="MTNEVFSWWFFLCAVSVLNGLAWLMSATKLGQRKGLFSAEVYAM
                     RRLQVLLSAGYVSGCAFRSALPVYDVQRMVIFDTWLSSVVVGRSVATIAELCFVAQWA
                     LMLREISRATGSNVGRTTSRVVVPLIAVAEVCSWYSVLTTSNLGHVVEESIWGVSAAL
                     MVASLAAIWPVCDAAVRRLLATWCAVGVGYVAFMFLVDVPMYWSRWLADEASGRHYLD
                     LAQGLSDVSQRWVVSHRWVDWKSEVVWMSLYFSVAVWLSIALIHAPVLVRRTALDPRA
                     RDWVTVATSGRHVPA"
     gene            complement(6283..6828)
                     /locus_tag="PKV56_14560"
     CDS             complement(6283..6828)
                     /locus_tag="PKV56_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOX69083.1"
                     /translation="MRLMDPERSVLVLVDYQQRLLPAIHRGAEVLAQAVRIADVAREL
                     GVPVYGTEQYPQGLGPNDEAIRSRCSSTLVKTHFDACADGLLELLPLDGSKAAREIVI
                     AGCEAHVCLLQTALGLLCADLRVSVIAQASGSRTAENHELAMQRLRQAGCEIVSVEMV
                     AFEWLRHCGHQRFKPVLKLLK"
     gene            complement(6849..7553)
                     /locus_tag="PKV56_14565"
     CDS             complement(6849..7553)
                     /locus_tag="PKV56_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX69084.1"
                     /translation="MIRPKEAVPGTATQIREAARTAARVERRQAVTDVRRSMVLAAAR
                     STFFELGLEGASLREIARRAGYTPGALYSYFASKEEVYGALLEESLERLNDRVSAAEA
                     APSSGRGAAARSRMAQGRLRAKSTAFFDFYRESPRDLDLGFYLFHGMQPRGLTPVLNE
                     QLNARLRDALAPAQQALLDLGLDADTAQAEVTALFAHIVGLLLLSHTGRIRMFRQESQ
                     ALFDRYLDDLLARHAA"
     gene            7679..>9085
                     /locus_tag="PKV56_14570"
     CDS             7679..>9085
                     /locus_tag="PKV56_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOX69085.1"
                     /translation="MSTAQLMTAADYRESLRRYRPTVYVDGQLIESVADAPSLQPGIN
                     ALGVTYDFAHDPKLAPLMTAIQTRSGKRVNRMLHINESAGDLLNKLEAVRLLCQETGC
                     AQRYLAHDALNAIGQVSARIDDAQGSTEHRARFSAYLQRVQDEDLSLGIAMTDAKGDR
                     SLRPHQQANPDTYVHVVERNARGIVISGTKAIVTGAPYMHEFLVMPCRNMGVQDADFA
                     VCCAVPVDASGITIVARPAGRPGDKVEHGAPLFSRRYGQSTGVVIFDRVFVPWERVFY
                     AGEWEHSGALTHHYATHHRHSCIGARAGFGDLLIGAGALMCEANGFDPGAKASLRDPM
                     VELIKITEGFFACGVAASVYGTHDEFSGSFMPEPVFSNIGKLLLSTQIYDMHRLAHEV
                     SGGLIVALPGPDEDHNPATAATLAEVLRANPAVPYDKRIEVARFIEDLTASYQGGWYS
                     VISLHGGGSPAAMKQEIWR"
BASE COUNT         1517 a         3066 c         3010 g         1392 t
ORIGIN      
        1 ctgcgaggta ggaggaacaa cgtgccaccg ttcgacggct tcgatctgga gtccttctgg
       61 gaagagtcgg aacacgcgac gaaggagtat gtcgccgcaa cgcctagccc tgctggcttg
      121 gcgaagatcg aggagcagtt gggagtcaag cttcccgctg cctacgtagc gcttgcgcag
      181 cgtcagaacg gtggaatccc tcgccggact gcgcatcgaa cgaccgagcg tacatcctgg
      241 gcggaagatc acattgcaat cagtggcatc tactcgatcg gtagtgccaa gccatgctct
      301 ctctgcggcc agtttggaag ctcgttctgg gtatcggagt ggggctaccc cgacatcggc
      361 gtctacttcg cggactgccc ttctgctggc cacgacatgc tttgcttgga ctatcgcgcg
      421 tgcggaccgc ttggagaacc tagcgtggtt cacgttgacc aagagttgga ctacaaggtc
      481 acgcgggtcg cccctacctt cgaggctttc gttcggggcc ttgaaccgga cgagtctttc
      541 aacgccgccc aactcaaacg ctgagtagca gctttgcgaa ctgaccggcc gttgattgag
      601 cccgggcaaa tttcagctct tggccaatag cagcccatcc gttcgagcag cccggtgatc
      661 ccaggcatca ccaacgaagt catccatcag caacactcga catcgagtcg cctgtccgcg
      721 ccgaacggca cgctggtgca cgcgatgccc cggaccggga actcggcgca gattccggtc
      781 gacccgttag tccaccactg gtcggtgaca acacacatcc gcgacgtgac gccgcaggag
      841 atcagccagg cggtggcgca gatggacttc ggcgcgccga agtgcggctg atggatgcgt
      901 tgtccacggg tgctgaccga caatccgctc catggccaaa cccgtcccgc gactgacccc
      961 ggatgaaatc gagcggatca tcgcgaccgc atgggacgac cggccgcctt tcgcggccgt
     1021 gctccgtcag cacgggctct cgccgggtca ggtggtgcag ttgctcaagc gcgagctgac
     1081 gccgaacgcg ttcaagatct ggaaggcgcg gacgcagggn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnna gcgtgatctc gggcacgctg gccagcgcct tcgacagcgg
     1261 gcactcgcgc ttggctgctg ccgcgatctc ctggaaccgg gcactgtcga ggcccggcac
     1321 ggtggcctgc atcgtcaacg caatgcggtc gatcacgaag ccctcgccct gcttcaccag
     1381 attcacgcgg gccgtcgtgt cgatcgaggt ggtggcgatg ccggctttgg cgcaagcgaa
     1441 ggaaaacgcc atcgtgaaac aggcggcgtg cgcggccccg agcagttctt cggggttggt
     1501 gccgagtcgg tcgtcttcga agcggctcgc aaagccgtag gggtggctct tgagagcgcc
     1561 ggtctcggtg ctgatctggc ccagaccgtt ctggccctga ccctcccagt gcacggtggc
     1621 ggttttttcg gtcatggtga atccttggtg gttggcaggt cagtcttccg gatcgacgac
     1681 acggtcgtgt tccgggtcgt ccgggatcac cggatggatc ggaccctggt cgggttcgag
     1741 cgggggaatg gcgggctcgt agggatcggg atcgtcgatg ggttggcggg gggatggggt
     1801 catggtccac tccgatgagc agcgttgcga tgaaccatcc tgcgaccgcc gtagtgcccg
     1861 tggggtggga ccgcaccgcg atgcgctgtc ggcgagcacc cacaagatcg aaggccaggc
     1921 cgcgctttgg cagaatcgaa cgccgccgat cggccgaggc ccgtcatgtc cgcgcatccc
     1981 ctgacgcacc acgagatcct gggcctgatc ggtccgttca cccggcgtgg gcgtcatctg
     2041 gacctgcctg cgagcgaccg gttggcccgg cgcctggtgt tcaagccgat cgagcatgcc
     2101 gacccggcga tcgcgtcgtc gacgctcatc gaaggcctgc agctcgagca gcccgaggac
     2161 gacacctacc ggctgacgcg cacgctcgcc ctcccctgcg gcctgcaggc cacgctgcag
     2221 accgatggca ccgatcccgc cgagctgctg gcgcggatcg aggccatccc gctgcagcgc
     2281 cagttccagc gcggcgcagg ctatgccgtc gcgctgtgtc atcgactgga tgcgacgaaa
     2341 ggcggtcccg ctgctgcgct gatggtgctg acgcgcgcca cggcgcgaat cgaagggctg
     2401 aggctggcga tgaaggtgcc tgcggtcacc ggcataccgg ccgacatcga actcgaggcc
     2461 gcccacggtg acagcgtcga tctgccgcag gacctgctgg cggtgctcgg ctggagctgg
     2521 gcgcgcctgg tgcgcgtcgg caaggcctgg acctgcagcc tgcgcctgcg cggcagcggg
     2581 ccccagcgca gccgcgacgc cgaggccaag ctggagcgca ccgcgcagca cctggcgcag
     2641 acattgtccg aacccccgcc gctctttcac gaccggcgcg tcgcggcacg ctggggcgtg
     2701 gtggtgcggc gctcgatccc gctgctggtg accgtctcgc tgatcggcct ggccgcggcg
     2761 gtgccgagca tggggctgtc ggaaaactcg gtgctgcgca tggtgatctt ccagtcaccg
     2821 cccctgctgc tgggcctggt gttctgcatg cgcgagctgc cgcagatcga gattccgccg
     2881 tggccgaggc gcccgtcgca gagtgcctgg ggtccgcagc gcgctcaggc aagcgtcccc
     2941 cgctgagccg cgccagcaag ccaacctgca aggtgaccga tgtccggcaa cccgatgaac
     3001 attcccccga tctcgatcgc cgccgcgaag gccgcgctga tcgagcagta cgccgacgcg
     3061 acgctgcgcc gccgcgcgac catgctgtgg ggcacgcgcg gcgtcggcaa gtcgtcggtg
     3121 gtgcgtcagg tggcggacca tttcaaggtg ccgctggtcg atctgcgcct cacgacgatc
     3181 gagccggtcg acatccgcgg tgcgatctac gccgacgagg tgcagggcaa gacggtgtgg
     3241 ttcccgccgg agttcctgcc cacgcccgac cagccggccg gcatcctgtt cctcgatgaa
     3301 ctgaccgccg ccgaccagcg gctgcagatc tcggcgtact cgctgatcct ggaccggcgc
     3361 gtcggcaact accggctgcc cgacggctgg caggtgatcg cggccggcaa cgccagcttc
     3421 cacggtgcgg tcagccacga catgggcacc gcgctggccg accggatgtt ccacttcaat
     3481 gtgcagacag tcatcgacgc gttcctggcg cacgcgctgg cgtgcggctt cgcacccgag
     3541 gtgatggcct acctgaaggt gcggcccgac cggctcgacg acaccccctc ccagctcgcc
     3601 aacgaccacc tggtcggcgc cagcccgcgc ggctgggaag acatctccaa tgtgctgaag
     3661 tcggggctgt ccgattcggc gaagcgggtc ttcgtgcagg gccgcatcgg cgccgccaat
     3721 gcggccgagt tcttcggtgt gctgcgcgag atccaggcag cggtcgacgt ggtgcggctg
     3781 ctcgccagtc gccccggcga cgagacggcg gcgctgctgc cgaagacgct cgatgggctc
     3841 tacggcatgc tctacgggct gctggccgcc tgcgtcgacg agaagaccgt ggcccgcgcg
     3901 ctggagatca tcgagcagct gcccgacgcc cgcgcgtcgg tgccgctgcc ggtacgcgag
     3961 tcgcagacgc tggcgatgga gctgctgatg cagaaggcgc tggagggcgg gctcgaagcc
     4021 gccatcctcg acagcccggt ctaccggcgc tatgccgagc agcgcagccg cgatggctga
     4081 acccggcgag ccgtaccagc atcggggcac ccgcgcgatc cagcgcatgg tcgagtacgc
     4141 gccggccacc ggcgggctcg cgctgtgggt ccggcaccag gacgtgcgac ccggtccggg
     4201 tgacatcgat acagcgccgg ttgtcacgac cgacggccac acgctgttct acgccgcagc
     4261 cttcgaggcg ttggcgctcg ccgaacagac cggctgggtc gcgcacgagg tgctgcacat
     4321 cgcgctgcgc catccgcagc gtttcctgga cctgaagcat ctggtcggcg atgtcgacct
     4381 gcagctgttc aatcgctgcg cggacgcgat cgtcaacagc acgctcggcc acctgagctg
     4441 gctgcagttg ccgtctacgg ccgtcacgct ggagtcactg ctcgcgaccg ccctgggcct
     4501 caagcgggtc gccgaggcgg cgctgctgga atgggatgtc gaaaggctct accgcgcgat
     4561 cgacgaccga cgcagcgacg ggcaggacgg ccggcgtgac ggcccgcgag cgaagaacgc
     4621 acgggcgctg ggagccgaca gccacgccga cctccagccc ggcgcgcagg tgcagtccgc
     4681 acctgaagac gaggccgaac aggcgcgcga atggagcgag cgcatcgcgc gcgcccacgc
     4741 caacgacggc gagttctcga tgtgacgcac gctgatcgcc gatgtgccga gaacgcgcat
     4801 accctgggag caggtactgc gcacccagct cgcccgccgg ctcgcaccga agcgcgacat
     4861 ctcctggtcg cggccgtcgc gttcgtacct cgccaaccag gggcgcgctg gcgcgcatcg
     4921 gcggatgccc tgggagccgg ggttcagttc ttcgaagtcg gtatcgcggc tggtggtgat
     4981 cgtcgatgtg tcgggttcga tcgaccccgc gctgatggag cgctttgcgc gcgagatcga
     5041 ggcgattacc cgaaggctcg aagccgggct ggtgctggtg atcggcgacg agcgggtgca
     5101 acgcgtggag cacttccagc ccggtcgcgc gaagctgagc gacatcccgt tcaagggcgg
     5161 tggcggcacc gacttcacgc cgctgctcga agaagccgac acgcaccgcc cggacatcgc
     5221 cgtcgtgctg accgacctgg atggcccggc ccgcttccgc ccgcgctggc cggtgatctg
     5281 ggccgtgcct caagcgaacg cagatgcgct tgcgcccttc ggtcgcaagc tggtgctgaa
     5341 ctgacagcgg ctcaggccgg gacgtggcgc cctgacgttg cgaccgtcac ccagtcgcgc
     5401 gcccgcgggt cgagcgccgt gcgacgcacc agcaccggtg cgtggatcag cgcgatgctc
     5461 aaccacaccg cgacactgaa gtacagcgac atccagacga cctcgctctt ccagtcaacc
     5521 cagcggtgcg acaccaccca gcgctgggag acatcggaca gaccctgtgc cagatcgagg
     5581 tagtggcgcc cactggcctc gtcggccagc cagcgcgacc agtacatggg cacatcgacc
     5641 aggaacatga acgcgacgta gcccacgccc accgcgcacc atgtggcgag caggcgccgc
     5701 accgccgcgt cgcagaccgg ccagatcgcg gccagactgg cgaccatcag ggcggcgctg
     5761 acgccccaga tcgactcctc gacgacatga cccaggttcg acgtggtcag caccgagtac
     5821 caggagcaga cctcggccac cgcgatcagc ggcaccacca cgcgggacgt ggtccgcccc
     5881 acgttgctgc cggtggcgcg cgagatctcg cgcagcatca aggcccactg ggcgacgaag
     5941 cacaactcgg caatcgtcgc caccgagcgt ccgaccacga cgctggacag ccaggtgtcg
     6001 aagatgacca tgcgttgcac gtcgtacacc ggcaaggccg agcgaaaggc gcacccggac
     6061 acgtagcccg ccgacagcag cacctgcagc cgtcgcatcg catagacctc ggccgagaac
     6121 aggcccttgc gctggccgag cttcgtcgcg gacatcaacc aggccaatcc gttcaggacg
     6181 ctgaccgcgc acaggaagaa ccaccaggag aaaacttcgt ttgtcatcac gctgtcattg
     6241 ggccatgcca gcgcgctgcg atcaatacgg agggacttct ggttacttca gcagcttcag
     6301 gaccggcttg aagcgctggt gcccgcagtg gcgcagccat tcgaaggcga ccatctcgac
     6361 gctgacgatc tcgcagccgg cctgccgcag gcgctgcatc gcgagttcgt ggttctcggc
     6421 agtccgcgaa ccgctggcct gcgcgatcac cgagaccctc aggtccgcgc acagcaggcc
     6481 cagcgccgtc tgcaacaggc acacatgggc ctcgcagcct gcgatgacga tctcgcgcgc
     6541 agccttcgac ccgtcgaggg gcaacagctc gagcaggccg tcggcgcagg catcgaaatg
     6601 cgtcttgacc agcgtcgaac tgcatcgcga gcgaatggct tcgtcgttgg gcccgaggcc
     6661 ttgcggatac tgctcggtgc cgtagaccgg aacgccgagt tcgcgggcga catcggcgat
     6721 gcgcaccgct tgcgccagaa cctcggcgcc ccgatgaatg gccggcagca ggcgttgctg
     6781 gtaatcgacc aggacgagca cggatcgttc agggtccatc agtctcatgg ggagatacct
     6841 cgaatgggct aggcagcgtg ccgcgccagc agatcgtcca ggtaacggtc gaacagcgcc
     6901 tgcgactcct ggcggaacat gcggatgcgg ccggtgtggc tgagcagcag caggccgacg
     6961 atgtgcgcga acagtgccgt cacctcggcc tgcgccgtgt cggcatcgag cccgagatcg
     7021 agcagcgcct gctgcgccgg ggccagcgcg tcacgcagcc gcgcgttgag ctgctcgttg
     7081 agcaccggcg tcagcccgcg tggctgcatg ccgtggaaca ggtagaagcc caggtcgagg
     7141 tcgcgcgggc tctcgcgata gaagtcgaag aacgcagtgg acttggccct cagcctgccc
     7201 tgagccatcc gcgatcgcgc ggcggcaccc cgcccggagc ttggcgcggc ctcggctgcg
     7261 ctgacccgat cgttcaggcg ctccagcgat tcctcgagaa gcgccccata gacctcctcc
     7321 ttgctcgcga aatagctgta gagcgcgccc ggcgtgtaac cggcgcgcct ggcgatctcg
     7381 cgcaggctgg ccccctcgag accgagctcg aagaaggtcg atcgcgcagc cgccagcacc
     7441 atcgagcgcc gcacatcggt gacggcctga cggcgctcga cgcgagccgc ggtgcgggcc
     7501 gcttcgcgaa tctgtgtagc cgtgccgggt acggcctctt tgggtcggat catgcagaaa
     7561 ttgtaggttc gaacaaattc ctgaacagcg ttcaggaaat tcgactcatc agctagagtg
     7621 gcgggaacag gcatgtcccg gcgccgctga ccgcgccatc attcaccggc acccacccat
     7681 gtccacagcc caactgatga ccgcggccga ctaccgcgag tcgctgcgcc gctatcggcc
     7741 caccgtctac gtcgacggac agctgatcga atccgtcgcc gatgcgccga gcctgcagcc
     7801 gggcatcaac gcgctcggcg tcacctacga cttcgcgcac gacccgaagc tggctccgct
     7861 gatgacggcg atccagaccc gcagtggcaa gcgggtgaac cgcatgttgc acatcaacga
     7921 atcggccggc gacctgctca acaagctcga ggcggtgcgg ctgctgtgcc aggaaaccgg
     7981 ctgcgcgcag cgctacctgg cgcacgacgc gctcaatgcg atcggtcagg tcagcgcgcg
     8041 catcgacgac gcgcagggca gcaccgagca ccgcgcccgc ttctccgcgt acctgcagcg
     8101 cgtgcaggac gaggatctgt cgctgggcat cgcgatgacc gacgccaagg gcgatcgctc
     8161 gctgcggccg caccagcagg ccaatccgga cacctatgtc cacgtcgtcg agcgcaacgc
     8221 ccggggcatc gtgatctccg gaaccaaagc catcgtcacc ggtgcgccct acatgcacga
     8281 gttcctcgtg atgccgtgcc gaaacatggg cgtgcaggac gccgactttg ccgtgtgctg
     8341 cgcggtgccg gtcgacgcca gcggcatcac catcgtggcg cgaccggcag gtcgccctgg
     8401 cgacaaggtc gagcacggag cgccgctgtt ctcgcgccgg tacgggcagt ccaccggcgt
     8461 cgtgatcttc gaccgggtgt tcgtgccctg ggaacgggtg ttctacgccg gggagtggga
     8521 acactccggc gccctcaccc accattacgc cacccaccac cgccacagtt gcatcggcgc
     8581 acgcgccggc ttcggcgatt tgctgatagg cgccggtgcg ctgatgtgcg aggccaacgg
     8641 tttcgatccc ggcgccaagg ccagcctgcg cgacccgatg gtcgagctga tcaagatcac
     8701 cgaaggcttc ttcgcctgcg gcgtggcggc cagcgtctat ggcacgcacg acgagttcag
     8761 cggcagcttc atgcccgaac cggtcttttc caacatcggc aagctgctgc tgtccacgca
     8821 gatctacgac atgcaccggc tggcgcacga agtgtcaggc ggattgatcg tcgccctgcc
     8881 cggccccgac gaggaccaca accccgccac ggcggccacg ctggccgagg tgctgcgcgc
     8941 caacccggcc gtgccgtacg acaagcgcat cgaggtggcg cgcttcatcg aggacctgac
     9001 ggcgtcgtac cagggcggct ggtattcggt gatcagcctg cacggcggcg ggtcgccggc
     9061 cgcgatgaag caggagatct ggcgc
//
LOCUS       DAOORV010000134         8960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9616_length_8960_cov_7.398765, whole genome shotgun sequence.
ACCESSION   DAOORV010000134 DAOORV010000000
VERSION     DAOORV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_8960_cov_7.398765"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="narH"
                     /locus_tag="PKV56_14575"
     CDS             <1..524
                     /gene="narH"
                     /locus_tag="PKV56_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803122.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOX69086.1"
                     /translation="QWKVALPLHPEYRTLPMVWYVPPLSPISAAANAGHVGSNGEIPD
                     VNQLRIPVKYLANLLTAGDTAPVVRSLERMLAMRAYQRAKHVDGRINQAVLNQVGITQ
                     AEVEEMYHVMAIANYEDRFVIPSTHREYAENTFNVRGGCGFSFGNGCSEGTGETSLFG
                     SEKKRTIPIKAEV"
     gene            527..1237
                     /gene="narJ"
                     /locus_tag="PKV56_14580"
     CDS             527..1237
                     /gene="narJ"
                     /locus_tag="PKV56_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOX69087.1"
                     /translation="MFGFDSPPKMSDSLRVLAALLGYPDAALRSHLPEMGKLLREEQA
                     VSPSRCAELYLLMESLRRADLLEAESAYVELFDRGRSTSLHLFEHVHGDSRERGPALI
                     DLGQTYASAGLFLAEGELPDYLPAVLEFVSTQPPREALAFLAEMSHIFSAIFGALLQR
                     DSAYASVLGALIELSGETAKPVTPASEPQLDESWAEPEVFGGCSTQGQAKPGQPQPIQ
                     IVKRSLAGAAPTSSGVRT"
     gene            1234..1971
                     /gene="narI"
                     /locus_tag="PKV56_14585"
     CDS             1234..1971
                     /gene="narI"
                     /locus_tag="PKV56_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOX69088.1"
                     /translation="MNTAVHNFLFNIYPYICLAIFLMGSLARFDRDQYTWKSDSSQLL
                     RAGQLRWGSNLFHGGILFLFFGHLFGQLTPHWLYEIFVTASQKQLVAVVAGGVAGVLC
                     FIGLTLLLHRRLFDPRIRLTSHRTDIAILLILWLQLSMGLATLPSSLHHVSEPTTMIT
                     LATYLQGVATLQPNAALVTGIEWHFQAHMLLGMTIFLLFPFSRLVHVWSGFGSVAYLF
                     RPYQVVRSRRLNVPAGHNQPRQPGAGV"
     gene            1985..2794
                     /locus_tag="PKV56_14590"
     CDS             1985..2794
                     /locus_tag="PKV56_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX69089.1"
                     /translation="MLSEAIDFSVPDTPVGTPPRINGVALNPLVQDLSPEDLRQRACT
                     ELLRQAAIRDGLLDAADQAPGDGVISDEASQAIDAWLERELKLPDPSDEACRRHFAAY
                     PARYRTGERVRVRHILFAVTPGMDVVKLRNRAEACLLDVRCHDGQASDGFARSARELS
                     NCPSGEQGGDLGWLSTADCAPEFARELFGHVEVGVLPRLVHSRFGLHVVEVLEREGGE
                     AQSFEAVRGAVEMALRQQAWVTALRQCLQMLAGVAVLEGVDLDAADTPLVQ"
     gene            2815..3567
                     /locus_tag="PKV56_14595"
     CDS             2815..3567
                     /locus_tag="PKV56_14595"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOX69090.1"
                     /translation="MPDELLQRLRRFHDDYFPGVQGQFQSLMQEGQHPTILFIGCSDS
                     RLVPYLLTGTGPGEVFMVRNVGAFVPPHDGSAGYHGTAAAIEFAVLKLNVARIVVCGH
                     SHCGGIRALYEEATPQATNLAAWLELGREAVLPVQVTAEALRRTEQRSVVLQLERLMG
                     YPMVRERVEAGRLTLHGWHYVIEDGEVHVFDVRSGSFLPASIAVHSGTGPFESAPVDD
                     GSSSFNEIDADLAPQSRSEIQAVRHDPAPSDE"
     gene            3602..4816
                     /locus_tag="PKV56_14600"
     CDS             3602..4816
                     /locus_tag="PKV56_14600"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOX69091.1"
                     /translation="MTRVALHGLAPLRLAGHHLLPIVQGGMGVGVSAHRLAGSVAALG
                     GVGTISSVDLRRHHPDLMERTRGLAPGKAATQAAKEAINAANLEALEREIHAAQVLAG
                     GRGLLAINVMRAVSEYGPSVTRALECGIDAVVVGAGLPLDLPELAQDHPTALLVPILS
                     DARGVQLLVRKWMRRNRLPDAIVIENPRLAGGHLGAARLDELNDPRFEFEVVIPQVIE
                     FLRAAGVEKDIPLIAAGGIRCFEDISRVQALGAAAVQLGTPFAVTLECDAAPAYKRVL
                     AQARDEDLIEFISGAGLPARAVPTPWLKNYLSVEGKLQEVAHAKPRCTLAFDCLLQCG
                     LRDGRPDWGQFCIDTQLAAALRGDEKKGLFFRGVGALPFGERIVGVHELMERLLTPIG
                     RLADLPDAVEPA"
     gene            4813..6117
                     /locus_tag="PKV56_14605"
     CDS             4813..6117
                     /locus_tag="PKV56_14605"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOX69092.1"
                     /translation="MKTIPVRAVQVGEPDGAPASEPPGWSLRWLVVAPHRIGFFAAAC
                     MLAVSALWWGTALLSRAAGHELPWAVAPPAAHALLMGLGFTPLFIAGFLFTAGPKWLG
                     EPAVDARMLCRPILLMLAGWCIVLPGLHLAAPWAAAGMVLVAGGWSRLCWRFLAILRA
                     SRVPDRSHAQVVATACMVGAVTMWVIVGALAGSDLTLLRAATQVALWCFVATVFVAVS
                     HRMIPFFSASALPALDAWRPMWLLWTMVAALWGEAFAAVAALGWGPLPFWMRGMQAAI
                     ELPVAVLLLWLALRWGVVQSLKIRMLAMLHAGFLWLGLAYALSALSHALMATTAGELS
                     LGLAPMHALTMGYLGATLLAMATRVSSGHSGRSLAADNTAWAMYWVLQAAVVLRVVAA
                     LWEAGSMGVTLLAVLAWTTATVAWALRYGRWFVRPRVDGRPG"
     gene            complement(6161..7255)
                     /locus_tag="PKV56_14610"
     CDS             complement(6161..7255)
                     /locus_tag="PKV56_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69093.1"
                     /translation="MNGPGAWRTGIVLSALLAAMNLLAGISGGLARLGLPTPAAAIGA
                     HGAVLACGFFGTLIALERAVALRRLAGLVAPLAAGLGGLLAWAVGVPAAAQLSWGIAA
                     IALVGLYVHAGCSRAWSVPLVVELCGALCWGVGTAAWAFGDLAAGVTGWMAFLVLTIA
                     AERRELTQMLRLPALAQRLFGIVVALALAAVVLDVAGTFGVGTGAWGAATWWSACALL
                     AMWLLRWDIAPRQWRKPGWLGHTAQCLTVGYLWLLAGGVLGLIGLGWPGGITVLARHA
                     VLLGFVFAMVFGHAPIILPALARVRPTYSAWARIPIWILTASLLARGAALATGSGAGL
                     SMAGAGHALAIVWFAAAMSSCVIRTRRLER"
     gene            complement(7252..7656)
                     /locus_tag="PKV56_14615"
     CDS             complement(7252..7656)
                     /locus_tag="PKV56_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HOX69094.1"
                     /translation="MTISSFDDLLRSASEQDQPQRLLFVFTTAALPDDATDEQRARFE
                     SGRGGTLTPLMCVDKATDELSSFADLREESSRFGQDWDIVFVAAMSGSAGIAPTSAQA
                     EAPLQGMVDAIKVGRIGSFIPFNGEGLPVQFT"
     gene            complement(7728..8042)
                     /locus_tag="PKV56_14620"
     CDS             complement(7728..8042)
                     /locus_tag="PKV56_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOX69095.1"
                     /translation="MTPDVSPTADSIRIALHEVLDPELGESIVDLGLVQRIGIEPGFV
                     RVVLIPTSATCPMSAVLLEDAADAVRKACPAGTRVEVELDWDTRWEPQRMSPELKVRF
                     GW"
     gene            complement(8039..8371)
                     /locus_tag="PKV56_14625"
     CDS             complement(8039..8371)
                     /locus_tag="PKV56_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX69096.1"
                     /translation="MALPHAQPLEVIDICPLGPALHDTATTSLLKTPRLQLMRLVLSA
                     GSGTPEHSVPGAITVHCLEGTAVVTTASRRCELHPGQLVMLPENERHAVQAVTDSSLL
                     VTLLLHQT"
     gene            8562..>8960
                     /locus_tag="PKV56_14630"
     CDS             8562..>8960
                     /locus_tag="PKV56_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOX69097.1"
                     /translation="MHPVENSPSHRIQDLPAQDISAEVLLEKYAKDDERSAEEVNRRV
                     ARALAQAEAPAERARWEARFAEALQAGFVPAGRIQSAAGTGLSATLINCFVQPVGDSI
                     AQPEDGHPGIYTALTEAAETMRRGGGVGYDF"
BASE COUNT         1357 a         2993 c         3116 g         1494 t
ORIGIN      
        1 tgcagtggaa ggtggcgctg ccgctgcacc ccgagtaccg cacgctgccg atggtctggt
       61 acgtgccgcc gctgtcgccg atcagcgcgg cggccaatgc cggtcacgtg ggttccaacg
      121 gcgagatccc cgacgtcaac cagctgcgca tcccggtcaa gtacctcgcc aacctgctga
      181 cggccggcga caccgcaccg gtggtgcgct cgctcgaacg catgctggcg atgcgtgcct
      241 accagcgagc gaagcatgtc gatgggcgca tcaaccaggc ggttctcaat caggtcggca
      301 tcacgcaggc cgaggtcgag gagatgtacc acgtgatggc gatcgccaac tacgaggacc
      361 gcttcgtgat cccgagcacc caccgcgaat acgccgagaa caccttcaac gtgcgcggag
      421 gctgcggctt cagtttcggc aacggctgct cggagggcac cggcgagacc agcctgttcg
      481 gcagcgagaa gaaacgcacc atacccatca aggcggaggt ttgatcatgt tcggtttcga
      541 ctccccaccc aagatgtcgg acagccttcg cgtgctggct gctctgctcg gctacccgga
      601 tgccgcgctg cgatcccacc tgcccgagat gggcaagttg ctgcgcgagg agcaggccgt
      661 gtcgccatcg cgatgcgccg aactctatct gctgatggag tccctgcgtc gtgccgatct
      721 gctggaggcc gagtccgcct acgtcgaact gttcgaccgt ggccgctcga cttcgcttca
      781 tctgttcgag cacgtgcacg gcgactcgcg agagcgcggt ccggcgttga tcgatctggg
      841 ccagacctac gccagtgccg gtcttttcct ggccgaaggt gaactgccgg actacctgcc
      901 ggcggtgctc gagttcgtgt ccacgcaacc tccgcgcgag gcgttggcat tccttgcgga
      961 gatgagccac atcttcagcg cgatcttcgg tgcgctgctg cagcgcgaca gcgcctacgc
     1021 cagcgtgctg ggggcgttga tcgaactctc cggtgaaacc gcgaagcccg tcacgcccgc
     1081 atccgagccc cagcttgacg agagctgggc cgagccggag gtgttcggtg gctgctcgac
     1141 gcaggggcag gccaagcccg gccagccgca acccatccag atcgtcaagc gctcgctcgc
     1201 aggcgccgcc cccacatctt caggagtccg cacatgaaca ccgccgtgca caactttctg
     1261 ttcaatatct acccgtacat ctgccttgca atcttcctga tgggcagtct ggcccgcttc
     1321 gaccgcgacc agtacacctg gaaaagcgac tcgtcgcagc tgctgcgcgc cggccagttg
     1381 cgctggggca gcaatctgtt ccacggcggc atcctgttcc tgttctttgg ccacctgttc
     1441 ggccagctca caccccattg gctgtacgag atcttcgtca cggcgtcaca gaagcagctg
     1501 gtggccgtcg tggcaggcgg cgtggctggg gtgctttgct tcatcggtct gacgctgctg
     1561 ctgcatcgac gcctgttcga tccgcgcatc cgactgacca gccaccgcac cgacatcgcg
     1621 atcctgttga tcctctggtt gcagttgtcg atgggcctgg ccacactgcc ttcgtcgttg
     1681 caccacgtgt cggagccgac caccatgatc acgctggcga cctacctgca gggtgtcgcc
     1741 accttgcagc ccaatgcggc cctggtcacg gggattgaat ggcatttcca ggcgcacatg
     1801 ctgctgggca tgacgatctt cctgctgttc ccgttcagcc ggctggtgca cgtgtggagc
     1861 ggttttggct cggtggccta cctcttccgt ccctaccagg tggtccgcag ccggcgcctg
     1921 aacgtgccgg ccggccacaa ccagccgcgc cagcctggcg ccggtgtctg aaggaggacc
     1981 tgacatgttg agcgaagcca tcgacttcag cgtcccggac acccccgtcg gcacaccacc
     2041 gcgcatcaac ggagtcgcac tgaatccgct ggtacaggac ctttctccgg aggacctgcg
     2101 ccaacgggcc tgcaccgagc tgctgcgtca ggcagccatc cgcgacggct tgctcgatgc
     2161 tgccgatcag gcgccgggcg acggcgtgat cagcgacgaa gcctcgcagg ccatcgatgc
     2221 ctggctggag cgcgaattga agctgccgga tccatcggac gaggcctgcc ggcgtcactt
     2281 cgcagcctac cccgcgcgct accgcacggg cgagcgggtg cgggtgaggc acatcctgtt
     2341 cgcggtgacg ccgggcatgg atgtcgtgaa gctgcgcaac cgggccgagg cctgcctgct
     2401 cgacgtgcgt tgccacgacg gtcaggccag cgatgggttc gcccgttcgg cccgcgaact
     2461 ctccaattgc cccagcggcg agcaaggcgg cgacctcggg tggctcagta ccgccgactg
     2521 tgctccggaa tttgcgcgcg agctgttcgg gcatgtcgag gtcggcgtgc tgccgcggct
     2581 ggtgcacagc cgcttcggcc tgcacgtggt ggaggtgctc gagcgggagg gtggcgaggc
     2641 gcagtctttc gaggcggtgc gtggtgccgt cgaaatggcc ttgcgccagc aggcctgggt
     2701 gacggcgctg cgtcagtgcc tgcagatgct ggccggcgtg gctgtgctcg agggtgtcga
     2761 cctggacgcg gcggatacgc cgctggtcca gtaggtcgga cagattcagc gcagatgcct
     2821 gacgaactgc tgcaacggct gcgccggttt cacgacgatt actttcccgg cgtccagggt
     2881 cagttccagt cgctgatgca ggaggggcag catccgacga tcctgttcat cggatgctcg
     2941 gactcccgcc tggtgcctta cctgctgacc gggaccgggc cgggcgaggt gttcatggtg
     3001 cgcaacgtcg gtgccttcgt cccgccgcac gacggctcgg cgggctacca cggcacggcc
     3061 gcggcgatcg agttcgcggt gctcaagctc aatgtggcgc gcatcgtcgt ctgtggccac
     3121 agccattgcg gcggcatccg cgcgttgtac gaggaggcca caccgcaggc caccaatctg
     3181 gcggcctggc tggagctggg tcgcgaggcg gtgctgcccg tgcaggtcac agccgaagcg
     3241 ctgcgccgta ccgagcagcg atcggtcgtg ctgcagctcg agcggctgat gggctacccg
     3301 atggtgcgcg aacgtgtcga ggcgggccga ctgacgctgc acggctggca ttacgtcatc
     3361 gaggacggcg aggtgcacgt cttcgacgtg cgcagcggca gcttcctgcc cgcatcgatt
     3421 gccgtccaca gtggcacggg ccctttcgag tcggcaccgg tcgatgacgg cagcagcagc
     3481 ttcaacgaga tcgacgcgga tctggcgcct cagagccgat ctgaaataca ggcggtgcgt
     3541 catgacccgg cccccagcga tgaatgaacc gacggcgcat gccgacagct ccgacgatct
     3601 gatgactcgg gtcgccctcc acggactcgc gcctttgcgc ctggccggtc accacctgct
     3661 gccgatcgtg cagggcggca tgggcgtggg cgtttccgcc caccggctgg ctggcagcgt
     3721 ggccgcgctg ggcggcgtcg gcacgatctc gtcggtcgac ctgcgtcggc atcatccgga
     3781 cctgatggag cgcacccgcg gcctcgcacc cggcaaggcc gcgacgcagg ctgccaagga
     3841 agcgatcaac gcagcgaatc tggaagccct ggagcgcgag atccacgcag cgcaggtgct
     3901 ggccggcggt cgtggcctgc tcgcgatcaa cgtgatgcgc gccgtcagcg aatacggccc
     3961 gtcggtgacg cgcgcgctcg agtgcggcat cgatgcggtg gtcgtcggcg cgggtttgcc
     4021 gctcgacctg cccgaactgg cgcaggacca tccgacggcg ctgctggtgc ccatcctgtc
     4081 cgacgcgcgt ggcgtgcagc tgctggtgcg caagtggatg cgcaggaacc gactgcccga
     4141 tgcgatcgtg atcgagaacc cgcgcctggc cggcggacat ctcggtgcgg ctcggctcga
     4201 cgaactgaac gatccgcggt tcgaattcga ggtcgtcatt ccgcaggtca tcgagttcct
     4261 gcgtgccgcg ggcgtcgaga aggacatccc gctgatcgct gccggcggca tccgctgctt
     4321 cgaggacatc tcgcgcgtcc aggcgctggg cgcggctgcc gtgcagctcg gcacgccgtt
     4381 cgccgtcacg ctcgagtgcg atgcggcgcc ggcgtacaag cgcgttctgg cgcaggcgcg
     4441 cgacgaggac ctgatcgagt tcatcagcgg agccgggctg ccggcgcgtg ccgtgccgac
     4501 cccgtggctg aagaactatc tgtcggtcga aggcaagctg caggaggtcg ctcatgccaa
     4561 gccgcgatgc acgctcgcct tcgattgcct gctccagtgc ggtctgcgtg acggccgtcc
     4621 cgactggggc cagttctgca tcgacaccca actcgccgcg gcgcttcggg gcgacgagaa
     4681 gaagggactg ttcttccgcg gcgtcggtgc cttgccgttc ggtgagcgca tcgtcggcgt
     4741 gcacgaattg atggagcgcc tgctgacgcc gatcggccgg ctggccgacc tgcctgacgc
     4801 cgtcgagccc gcatgaagac gatcccggtg cgcgccgtgc aagtcggcga gccggacggc
     4861 gctccggcat ctgaaccgcc gggctggtcg ctgcgctggc tggtggtggc accgcaccgg
     4921 atcggcttct tcgctgcggc ctgcatgctg gccgtttccg cgctgtggtg gggcaccgcg
     4981 ctgttgtcgc gtgccgcggg gcacgaactg ccatgggccg tggcgccgcc ggcggctcat
     5041 gcgctgctta tgggcctggg cttcacacca ttgttcatcg ccggcttcct gttcaccgcc
     5101 ggtccgaaat ggctgggtga accggcggtc gatgcccgga tgctgtgccg gccgatcctg
     5161 ctgatgctgg ctggctggtg catcgtgctg ccgggcctgc acctggctgc accctgggcc
     5221 gccgccggga tggttctggt ggcgggcgga tggagccgac tgtgctggcg attcctcgcg
     5281 atcctgcggg cgagtcgggt tcccgaccga tcgcatgcgc aggtggtggc cacggcctgc
     5341 atggtcggcg cggtgacgat gtgggtgatt gtcggcgcgc ttgccgggtc cgatctcacc
     5401 ctgttgcgcg cggccacgca ggtggcgctg tggtgcttcg tggcgacggt gttcgtcgcg
     5461 gtgtcgcacc gcatgattcc gttcttcagc gccagtgcgc tgccggcgct cgatgcctgg
     5521 cgtccgatgt ggttgctgtg gacgatggtg gcggcgctct ggggcgaggc gttcgcggcc
     5581 gtcgccgcac tcggttgggg gccgttgcct ttctggatgc gtgggatgca ggccgcgatc
     5641 gaattgccgg tcgcggtgct tcttctctgg ctggcgctgc gctggggcgt ggtgcagagc
     5701 ctgaagatcc ggatgctggc gatgctgcat gccggcttcc tgtggctggg tctggcgtac
     5761 gcgctgtcgg cgctgtcgca cgcgctgatg gcaacgaccg ctggcgaact gtcgctgggc
     5821 ctggcgccga tgcacgcgtt gaccatgggc tacctcggcg ccacgctgct ggcgatggcc
     5881 acgcgcgtga gcagtggcca cagcgggcgc tcgctcgcgg ccgacaacac agcttgggcg
     5941 atgtactggg tgctgcaggc tgcggtggtc ctgcgggtgg tggcggcgct gtgggaggca
     6001 ggctccatgg gggtcactct gcttgcggtg ctggcctgga ccaccgcaac cgtggcttgg
     6061 gcgctgcgct atggccgctg gtttgtgcgc ccgcgcgtcg acggccggcc cggctgatcg
     6121 gcgcgagggt ccgtcgatcg agctttcgtt cctgcgaacg tcagcgttcc agtcgacgag
     6181 tgcggatcac gcatgacgac atcgctgccg cgaaccagac gatggcgagg gcgtggcctg
     6241 cgcccgccat cgacagcccc gcgccggaac ccgtggccag tgcggcgcca cgtgccagca
     6301 ggctggcagt gaggatccag atcgggatgc gggcccaggc cgaataggtc ggacgcacgc
     6361 gcgccagcgc cggcaggatg atcggtgcgt gtccgaacac catcgcgaac acgaagccca
     6421 gcagcaccgc gtgcctcgcg agcacggtga tgccgccggg ccatccgaga ccgatcaggc
     6481 ccagcacgcc accggccagc aaccacaggt atccgacggt caggcactgc gcggtgtggc
     6541 ccagccagcc gggcttgcgc cactggcgtg gcgcgatgtc ccagcgcagc agccacatcg
     6601 cgagcagcgc gcaggcggac caccaggtgg cagcccccca cgcgccggtg ccgacgccga
     6661 aggtgccggc gacgtccagc acgacggcag ccaatgccag tgccacgacg atcccgaaca
     6721 agcgctgcgc cagcgcgggc agccgcagca tctgcgtcag ctcacggcgt tcggctgcga
     6781 tcgtcagcac aaggaaggcc atccagccgg tgacgccagc ggccaggtcg ccaaacgccc
     6841 acgctgcggt gccgacgccc cagcacagag cgccgcacag ctcgacgacc agcggcacgg
     6901 accacgcgcg gctgcagccc gcatggacgt agaggccgac cagcgcgatg gctgcgatcc
     6961 cccatgacag ttgcgctgcc gctggcaccc ccacggccca ggccagcaat ccaccgaggc
     7021 ccgctgccag cggggccacc aatccagcca ggcggcgcaa ggccacggca cgctccagcg
     7081 cgatcagcgt gccgaagaac ccgcaggcca gcaccgcacc gtgcgcaccg atcgcagcag
     7141 cgggtgtcgg cagacccagc cgcgccaggc caccactgat gccggccagc aggttcatcg
     7201 cggccagcaa ggcgctgagg acgatgccgg ttcgccaggc ccccgggccg ttcacgtgaa
     7261 ctgcacgggc aggccttcgc cgttgaacgg gatgaacgat ccgatgcgac ccaccttgat
     7321 ggcgtcgacc atgccttgca gcggtgcttc ggcctgcgcg ctggtcggtg cgattcccgc
     7381 gctgcccgac attgcggcaa cgaagacgat gtcccagtct tgcccgaagc gacttgactc
     7441 ttcacgcaag tcggcaaacg aggacagctc gtcggtcgcc ttgtccacgc acatcagcgg
     7501 cgtcagcgtg ccgccccggc cggactcgaa gcgcgcgcgc tgctcgtcgg ttgcgtcgtc
     7561 cggcagcgcg gcggtggtga aaacgaacag caggcgctgc ggctggtcct gttcgctggc
     7621 cgagcgcagc aggtcgtcga agctggaaat ggtcatggca gcgatgtgaa gtgggtgggg
     7681 ggatgaaaaa agaccgggga gtgtgcccgt cgccagcagt gcggagctca ccagccgaag
     7741 cggactttca gctcggggct cattctctgc ggctcccagc gcgtgtccca gtcgagttcg
     7801 acctcgacgc gtgtcccggc aggacaggcc tttcgcaccg cgtcggcggc gtcctcgagc
     7861 agcaccgcgc tcatcgggca ggttgcgctg gtcgggatga ggacgacgcg cacgaacccg
     7921 ggctcgatcc cgatgcgttg caccaggccg aggtcgacga tgctctcgcc gagttcgggg
     7981 tcgagcacct cgtgcagcgc gatgcggatg ctgtcggcgg tcggggagac gtccggggtc
     8041 atgtctggtg cagcagcaag gtgacgagca gcgacgagtc ggtgaccgcc tgaaccgcat
     8101 ggcgttcgtt ctcgggcagc atcaccagct gaccggggtg caactcgcag cggcgggatg
     8161 ccgtcgtgac gacggccgtg ccctcgaggc agtgcacggt gatcgcaccg ggcacgctgt
     8221 gctccggcgt gcccgagcct gcgctgagca ccagacgcat cagctggagc cggggagttt
     8281 tcagcaggct ggtcgtcgcg gtgtcgtgca gcgcggggcc caacgggcag atgtcgatga
     8341 cttcgagagg ttgcgcgtgg ggcagggcca tggtgcctcc gtgtcgtgcc gggaaggctc
     8401 gccgagggta tcgaaattgc agacacgtgg ccagcagggt cttgcgcgcc gccgggtccg
     8461 taacatctgt gcagcaggtg tctgcgacct tgacgggcat caaggatcga aagcgtcgca
     8521 tcaccaaact tgccccctcg aatcaaacca gaagccgctc catgcacccc gtcgaaaact
     8581 cgccatcgca ccgcatccag gatctgcccg cccaggacat cagcgccgag gtcctgctcg
     8641 agaaatatgc aaaggacgac gaacgttcgg ccgaggaggt caaccgccgc gtggcgcggg
     8701 cgctggcgca ggccgaggcc ccggccgagc gcgcccgatg ggaggctcgt ttcgccgagg
     8761 cgctgcaggc cggcttcgtg ccggccggtc gcatccagtc cgccgccggc acgggcttgt
     8821 cggcgacgct gatcaactgc ttcgtgcagc cggtcggcga ttcgatcgca cagccggagg
     8881 acggccaccc gggcatctac accgcgctga ccgaggcggc cgagaccatg cgccggggcg
     8941 gtggcgtggg ctacgacttc
//
LOCUS       DAOORV010000135         8856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9784_length_8856_cov_8.204295, whole genome shotgun sequence.
ACCESSION   DAOORV010000135 DAOORV010000000
VERSION     DAOORV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9784_length_8856_cov_8.204295"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKV56_14635"
     CDS             <1..297
                     /locus_tag="PKV56_14635"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX69098.1"
                     /translation="TALDIVMAGVPALVVPYETATENEQRARAERLAALGAVQCLEPG
                     ALQPDAMVAAIERLLSFEPRPAALALDGAERTAQILVDCLQRLRSDTPFSRQAA"
     gene            294..1037
                     /locus_tag="PKV56_14640"
     CDS             294..1037
                     /locus_tag="PKV56_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69099.1"
                     /translation="MSEGLEPLLEVLERARQPVDCFIRDDDGGWDNERQIALLDATAA
                     AAVPIDLAVIPMALDGALARELIRRIDGGMQPLGVHQHGLSHTDHQTETGGRKCEFGP
                     AREPELQRLDLANGWNRLLRQFGPRLDPIFTPPWNRCAAETPHVLAGLGYAALSRSRG
                     ATAQQVLPELPVDLDWCKQHRAGGPEAIVHAWARDLGARIDDGLPFGLMLHHAAMEAA
                     EFDLLRSWLLELAHHPLLRWRSMRSLLAE"
     gene            1082..2539
                     /locus_tag="PKV56_14645"
     CDS             1082..2539
                     /locus_tag="PKV56_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOX69100.1"
                     /translation="MNRLTPAISGLLFASVLLSANAQVAPADAATTAVSRQGLRMDDR
                     RPENPFTIDLLGRPVEITGSWEYSDERRRNFDLQSSRLRDRRVREHELKLEARVRPTA
                     DTEVFVEAVGLHETRHTQGSPRAQRNGAHERGQSWLRIDRLGGTNWSMQIGRVALIDR
                     RSWWWDDDLDAVRLIGSGDGWRLDTGVAHQVARVSSDERRTPPEARGVTRWFGQATWE
                     LARRHRLDGFWLIERDDSRRPIAGSSAAGSDSIDPSDFRGHWIGARSSGEWRPDAGLR
                     IGYWADIAWVHGREALTGYTESDDGRFTAGTTRKRRVRGHAYDVGTTAIFPIWLRPSV
                     TVGYARGSGGTRSSSRDSNFRQTGLQENKARLAGVKRVQTYGELLQPELSNLGVSTLA
                     AGVRLTDNTSLELIAHQYRQPVASSTLPGSRLSADPQGRSADIGREIDLVLSVRESRR
                     LEFTLKWSHFRPGAAFAPEQRDPARALELGVALNF"
     gene            complement(2607..2683)
                     /locus_tag="PKV56_14650"
     tRNA            complement(2607..2683)
                     /locus_tag="PKV56_14650"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2647..2649),aa:Val,seq:gac)
     gene            complement(2737..3969)
                     /locus_tag="PKV56_14655"
     CDS             complement(2737..3969)
                     /locus_tag="PKV56_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69101.1"
                     /translation="MEAPRIDELIAQVVAWHNRHGLARRIASTHVRSIGVVVLPFVSP
                     VSATAKGSDKDLVPAFSEDFMAPLTPKQVARFAVRLGSREHPGAMSWAQRVVPVDPGH
                     EASELVLLYLRTASIELGDHRCRVLIGSGPRPKIIGPRAWSPIRIAGASAAATAVMAS
                     GLMASILLGSPAQPESLVAAVSPAASTPEHAVHLSKQAASAPLAASGPGLSSAPEAAS
                     AAAAHAFQSPLAAIAASASMPVAGASSVARAASGVRTSIASIARPASAPAEASAPKPA
                     VERTADADASNDRFRAPAIRPALSGEVRAAALLEGKRLREMAPPGKEGAAVPGSGHVY
                     AVMTISTRTRAVAERGHLVMDSSATAGELPGKPRSELMQVSGGWRAVLWPFSSRDDAK
                     VAQAMLSERGVRTEVLEF"
     gene            complement(4039..6300)
                     /locus_tag="PKV56_14660"
     CDS             complement(4039..6300)
                     /locus_tag="PKV56_14660"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOX69102.1"
                     /translation="MKKPVPAAHAGAVAIVLLADGGGADAATQGDVVSEVQRARAFAQ
                     PLLVGQLLDTGEDALEHADGVCAILEGIGAAPSMRAAAYLVYAGDYLQKPEETVAKAF
                     GPSHASLVVHTRKLVQIQRAAREAQLEGEQRAQQTERVRKMLLAFARDLRVVLLRLAS
                     RLQTLRYYAASKQRCPELLARESMQVFAPLANRLGIWQIKWEIEDLAFRFLEPEKYKA
                     VARLLDEKRVERVQHVESFRQRLSEQLRDCGIAAEVQGRPKHLYSIWKKMQGKGLDFD
                     RVLDVRAMRVIVGSVPDCYAALSRVHELMPPVADEFDDYIARPKPNGYQSLHTVVLDD
                     AGRPVEVQIRTQAMHDHAEHGVAAHWAYKEAGVKGYAGVVAADSGFDAQLAEARKGVL
                     RQLLAWERDFVGQEESAAQSQAGVTFDDRIYVFTPQAAVVELTAGATPIDFAYAVHTD
                     LGHRCRGAKVDGTMVPLNTPLANGQTVEVTAAKEGGPSMDWLNADLGYLQSARSKAKV
                     RAWFNARAQAETIARGREAVEKLLQRIGKTAVKLDDLAGQLGFRQADALFEVVGKDEF
                     SLRTIETLLKPAEPQPTPDDLIAQRKSRPDGESPKGGVLVVGVESLLTTLSRCCRPAP
                     PDAIGGYVTRGKGVAIHRHGCSNFKQMALRAPERVISVAWGTPRGGKDALYPVDVTVQ
                     ASDRQGLLRDITEVFSKDRMNVTSVKTQSFKDTGGGTAWMTFTVEVSDAARLAHVLGL
                     VGLVAGVRSVRRK"
     gene            complement(6297..7166)
                     /locus_tag="PKV56_14665"
     CDS             complement(6297..7166)
                     /locus_tag="PKV56_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX69103.1"
                     /translation="MNKPRLHHVPCLDTQGLHRTAYWEWGDPSNPRVLVCVHGLTRQG
                     RDFDVLAAAMSDHYRVVCPDIAGRGESDRLTNPMEYGIPRYVADMVTVLARVHAETLH
                     WVGTSMGGLIGLGLAALAASPVSRLVLNDVGPVLEAKAIARIGAYLGLPLRWDSVEQA
                     AEYLLTISKGFGPHTAEQWLALTRPMLRPAEDGGVQLHYDPALAAPFKVTTPEMAAAG
                     EAALWHAYDAITCPTLLLRGADSDLLTAETAAAMTVRGPRARLHQFDGVGHAPTLVAP
                     DQVQVVREFLLSE"
     gene            complement(7213..7950)
                     /locus_tag="PKV56_14670"
     CDS             complement(7213..7950)
                     /locus_tag="PKV56_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837012.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOX69104.1"
                     /translation="MSLLRFPASLFRLRLAWLPVCAAAFCAGTTLAAEPAVPTGVVAL
                     TATASVEVTRDLMAVTLTATREGSDAHAVQAALKQALDGALAEAKSVAKPGQVDVRTG
                     NFSMYPRYSTKNALTGWQGTAELVIEGRDLQAIGQLAGRIQTMTIARVTQGLSREQRE
                     KAEGSVMAQAISRYRAQAAQVTQQFGYSSYVIREVTVGSNDQGGAPVPMLASARSMAL
                     TADAPLPVEAGQTSVSVSVSGTVQMLK"
     gene            8136..>8856
                     /gene="ompR"
                     /locus_tag="PKV56_14675"
     CDS             8136..>8856
                     /gene="ompR"
                     /locus_tag="PKV56_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HOX69105.1"
                     /translation="MTAATPARADRVVVVDDDARIRDLLRRYLTQEGFEVLLAEDAKA
                     LNRVLTRETIDLIVLDLMLPGEDGLSICRRLRAANDLTPIIMLTAKVEDVDRIVGLEV
                     GADDYLPKPFNPRELLARIHAVLRRRPSLEAPGAPAKEALTVTFGPFEFDLALRRLSK
                     QGEQMALTTGEFSMLKALVRHPRQPLSRDKLAQLARGREFEPFDRSLDVQISRLRKMI
                     EPDPAQPRYIQTVWGVGYVFVP"
BASE COUNT         1494 a         3047 c         2896 g         1419 t
ORIGIN      
        1 accgctctgg acatcgtgat ggctggtgtg cccgcactcg tcgtgcccta cgaaacggcg
       61 accgagaacg agcaacgcgc gcgcgcggag cgtctggccg ccctcggcgc ggtgcaatgc
      121 ctggagcccg gcgcattgca gcccgatgcg atggttgcgg caatcgaacg tctgctgtca
      181 ttcgagccgc gtccggcagc gcttgcgctc gacggtgccg agcgcacggc gcagatcctg
      241 gtcgactgct tgcagcgcct gcgatcggac acacctttca gtcggcaggc cgcatgagcg
      301 aaggcctgga accgttgctc gaggtgctcg agcgtgccag gcagcctgtt gactgcttca
      361 tccgcgacga tgacggtggc tgggacaacg aacgccagat cgccctgctc gatgccacgg
      421 ccgcggcagc cgttccgatc gatctggcgg tcattcccat ggcgctggat ggcgcattgg
      481 cccgcgagct gatccgacga atcgacggcg gaatgcaacc gctgggagtt catcagcacg
      541 gtttgtcgca caccgatcat cagaccgaga ccggcggacg caaatgcgag ttcggcccgg
      601 cacgcgagcc cgagcttcag cggctggacc tggcgaatgg ctggaaccgg cttctgcgcc
      661 agttcggccc tcggctggac ccgatcttca ccccgccgtg gaaccgctgc gccgccgaaa
      721 cgccacatgt tcttgccggg ctgggttacg cggcgctttc gcgcagccgc ggcgccacgg
      781 cacagcaggt actgccagaa ctgccggtgg acctggactg gtgcaagcag caccgcgctg
      841 gaggtcctga agcgatagtc catgcatggg cccgggacct tggcgcacgg atcgacgacg
      901 gcctgccctt cggactgatg cttcatcacg cggcgatgga ggcggccgag ttcgacctgc
      961 tccggagctg gctcctcgaa ctggcgcatc acccgctgct gcgctggcga tcgatgcgat
     1021 ccctgcttgc cgaataacgc gtccgcacct gcgcgacggc cttaagccca gcccccagct
     1081 catgaaccga ttgactcccg ccatttccgg cctgctgttc gcaagcgtgc tcctcagcgc
     1141 gaacgcccag gtggctcctg ctgatgcagc caccacggcc gtgagccgac agggactgcg
     1201 catggacgat cgtcgacccg agaacccctt cacgatcgat ctgctcggtc gcccggtcga
     1261 gatcacgggc agttgggaat acagcgacga gcgaagaagg aacttcgacc tgcaaagcag
     1321 ccgcctgcga gaccgccggg tgcgggagca cgaactcaag ctggaggcgc gcgtgcgccc
     1381 caccgccgac accgaggtgt tcgtcgaagc cgttggtctg catgagacgc ggcacacgca
     1441 gggctcaccg cgtgcccaac gaaacggagc gcacgagcga ggccagagct ggctccgcat
     1501 cgatcgcctg ggcggtacga attggtccat gcagatcggc cgtgttgccc tgatcgaccg
     1561 gcgctcctgg tggtgggacg acgacctgga tgccgtgcga ctgatcgggt ccggtgatgg
     1621 ctggcggctg gacaccggcg tggcgcatca ggttgcaagg gtctcgtccg atgaacgccg
     1681 cactccgccc gaagcacgcg gggtgacccg ctggttcgga caggccacgt gggagctggc
     1741 ccgacgccat cgcctcgacg gcttctggct catcgagcgc gacgactcgc ggcggcccat
     1801 cgctggctcg agcgcggcgg gctcggattc gatagacccg agcgatttcc gcggtcactg
     1861 gataggcgcg cgctcgagtg gcgaatggcg tccggacgct ggtctgcgca tcggctactg
     1921 ggcggacatc gcctgggttc acggacggga agcactcacc ggctacacgg agagcgacga
     1981 tggccggttc accgccggca cgacacgaaa gcgtcgagtg cgcggccacg cgtacgacgt
     2041 cgggacgacc gccatcttcc cgatctggct gcgtccttcg gtcaccgtgg gctatgcccg
     2101 aggctcgggc ggcacgcgca gcagcagccg ggacagcaat ttccgccaga ccggccttca
     2161 ggagaacaag gcccgccttg ccggcgtgaa acgcgtgcag acctacggag aactgctgca
     2221 acccgaactc tcgaatctgg gggtctcgac actggccgcg ggcgtgcgcc tgaccgacaa
     2281 cacctcgctc gaactgattg cgcaccagta tcgacagccc gttgcctcga gcaccctgcc
     2341 gggatcgcgc ctgagcgccg acccgcaggg ccgcagcgca gacatcggcc gggaaatcga
     2401 tctggtgctc tcggtgcggg aatcgcggcg gctggagttc acgctcaagt ggtcgcactt
     2461 ccggcccggc gctgcgttcg cacccgaaca gcgtgatccg gcacgcgctc tcgaactggg
     2521 agtggccttg aacttctgac ccgtccgact cgcgcggccc accaacgaaa aagccccgac
     2581 ctctcatgag atcggggctg tgttcttggt aggcgcgaat ggactcgaac catcgacccc
     2641 caccatgtca aggtggtgct ctaaccagct gagctacgcg cctaagagcc cgtgactata
     2701 acaggctgca ggcagcctgt gaacccgctc gcacggtcag aactcgagaa cttcggtacg
     2761 gacgccgcgt tccgagagca tcgcctgcgc caccttcgca tcgtcgcggc tgctgaaagg
     2821 ccagagcacg gctcgccaac caccggacac ctgcatcagc tcactgcgcg gcttgccggg
     2881 caactcgccg gccgttgcgc tcgaatccat gacgagatgg ccgcgctcgg ccactgcgcg
     2941 tgtgcgggtg gaaatggtca tgacggcata cacgtgacca gatccgggta cagcggcccc
     3001 ttccttgccg ggcggcgcca tctcacgcag ccgcttgcct tcgagcaagg cagccgcgcg
     3061 gacttcgccg ctgagcgcgg gacgaatggc cggcgcgcgg aagcggtcgt tcgatgcatc
     3121 ggcgtcagcc gtgcgctcaa cggcgggctt gggcgctgaa gcctcagcgg gcgcggaggc
     3181 tggccgcgcg atcgacgcga tggacgtcct gacccccgac gctgcccgag ccaccgagga
     3241 ggcaccggca acaggcatcg acgccgacgc cgcgatggca gccaacggcg actggaaggc
     3301 gtgggctgcc gcagcgctgg ccgcctctgg cgcgctcgac agcccaggtc cggatgcagc
     3361 gagcggcgcg gaagcagctt gcttcgagag gtgcactgcg tgttcgggag tggaagcggc
     3421 gggggaaacg gctgccacca gggattccgg ctgggctggc gatccgagca ggatcgacgc
     3481 catgagcccg ctggccatca cggccgtcgc ggcggcggag gctcctgcaa tgcggatcgg
     3541 gctccaggct ctcggtccga tgatcttcgg gcgtggcccg gagccgatga gcacgcgaca
     3601 ccggtgatcg ccgagttcga tgctggcggt tcgcaggtag agaaggacaa gttcggacgc
     3661 ttcatggccg gggtcgacgg gtaccacccg ctgtgcccac gacatcgcgc ccgggtgctc
     3721 cctgctgccc agccgcacgg caaaccttgc gacctgcttc ggcgtcaggg gcgccatgaa
     3781 gtcctcgctg aacgcgggga cgagatcctt gtcagagccc ttcgcggtcg ccgagacagg
     3841 cgagacgaag ggcaggacca ccacgccgat cgagcggaca tgcgtcgagg cgattcgacg
     3901 cgccaacccg tgacggttgt gccatgccac tacctgggcg atcagttcgt cgatgcgcgg
     3961 cgcttccata tgtaccgcgg attgtcccgg cgcgaccgca ccgtcgaaga ccggaacaga
     4021 ccggtcggag gcacccaatc acttgcgtcg aaccgatctc acacccgcca ccaggccgac
     4081 cagacccagc acatgggcca gccgggcggc gtcggatacc tcgacggtga aggtcatcca
     4141 cgcggtcccg ccaccggtgt ccttgaacga ttgcgtcttc acgctggtga cgttcatgcg
     4201 gtccttcgag aacacttcgg tgatgtcacg cagcagccct tgtctatcgg aagcctggac
     4261 cgtcacatcg acggggtaga gcgcatcctt gccgccgcgt ggggtgcccc aagccacgct
     4321 gatgacacgc tcgggcgcgc gcagggccat ctgcttgaag ttggagcagc cgtgacgatg
     4381 gatagccacc ccttttccac gggtcacata gccgccgatc gcatcgggtg gcgccggccg
     4441 acagcagcgc gacaaggtcg tcagcagcga ctcgacaccg acgacgagca cgccgccttt
     4501 cggagattcg ccgtcgggcc tgctcttgcg ctgcgcgatc aggtcgtcgg gcgtcggctg
     4561 aggctcggcg ggcttgagca gtgtctcgat cgtgcgcagc gagaactcgt ccttgccgac
     4621 cacctcgaac aacgcgtcgg cctgccggaa tccgagctgg ccggccaggt cgtcgagctt
     4681 gacggcggtc ttgccgatgc gttggagcaa cttctccacg gcctcgcgtc cgcgggcgat
     4741 tgtctcggcc tgcgcccttg cgttgaacca ggcgcgcacc ttggccttgg atcgcgcgct
     4801 ctgcaggtaa ccgaggtcgg cgttcagcca gtccatcgac ggcccgcctt ccttggctgc
     4861 cgtgacctcg acggtctggc cgttcgcaag cggcgtgttc agcggcacca tcgtgccgtc
     4921 gaccttggcg ccgcggcagc ggtgaccgag gtcggtgtgc accgcatagg cgaaatcgat
     4981 cggagtggcg ccagccgtga gttcgacgac cgcggcctgc ggcgtgaaga catagatgcg
     5041 gtcgtcgaag gtcacgccgg cctggctctg ggctgccgat tcttcctgcc cgacgaagtc
     5101 gcgctcccag gccagcagct ggcgcagcac gcccttgcgg gcttcggcaa gctgagcatc
     5161 gaagccggag tccgccgcca cgaccccggc atagcccttg acgccggcct ccttgtaggc
     5221 ccagtgcgcg gccacgccgt gctcggcatg atcgtgcatc gcctgcgtac ggatctgcac
     5281 ctcgactgga cggcccgcgt cgtccagcac gacggtgtgc agtgactggt agccgttggg
     5341 cttgggccga gcgatgtagt cgtcgaactc gtctgcgacc ggcggcatca gctcatggac
     5401 gcggctcaat gccgcgtagc agtcgggcac gctccccacg atgacacgca tcgcccgcac
     5461 atcgagcacg cgatcgaagt cgaggccctt gccctgcatc ttcttccaga tgctgtacag
     5521 gtgcttcggc cgtccctgaa cctcggcagc aatgccgcaa tcgcgcaact gttcggacag
     5581 ccgctggcga aaggactcga cgtgctggac ccgctcgacg cgcttctcgt cgagcagcct
     5641 ggccacggcc ttgtactttt cgggctccag gaagcggaac gccaggtcct cgatctccca
     5701 cttgatctgc catatgccga gtcggttggc gagcggcgcg aacacctgca tcgactcccg
     5761 ggccagaagc tcggggcacc gttgcttgct ggctgcgtaa taccgcagcg tctgcaggcg
     5821 cgaagccagg cgcagcagca ccacacgaag atcgcgtgcg aaggccagca gcatcttgcg
     5881 cacgcgctcg gtctgctgcg cccgctgctc accctccagc tgcgcctcgc gagcagcccg
     5941 ctggatctgc accagtttgc gggtgtgcac gacgagactg gcgtgcgacg gcccgaaggc
     6001 tttcgccacg gtttcttcgg gcttctgcag gtagtcaccg gcatagacca ggtacgccgc
     6061 tgcacgcatc gaaggcgccg cgccgatgcc ttcgaggatc gcgcagacac cgtcggcgtg
     6121 ctccagagca tcttctccgg tgtccagaag ctggccgacc agcaggggct gcgcaaatgc
     6181 ccgggcgcgc tgaacctcgg acacgacgtc gccctgagtg gcggcatcgg ccccaccacc
     6241 atcggccagt agaacgatcg caaccgcgcc ggcatgcgcg gcaggcacag gcttcttcat
     6301 tcgctcagca ggaactcccg cacgacctga acctgatccg gcgcgaccag cgtcggcgca
     6361 tggcccacgc cgtcgaactg gtgcaaccgc gcacgtgggc cccggacagt catcgccgcg
     6421 gcggtctcgg cagtcagcag gtcggagtcc gcgccgcgca gcaacagcgt cggacaggtg
     6481 atggcgtcat aggcatgcca cagcgcggcc tcgccggctg cagccatctc cggcgtcgtc
     6541 accttgaacg gagcggccag tgccgggtcg tagtgcagct ggacaccacc gtcctcggca
     6601 ggccgcagca tcggccgcgt cagcgccaac cattgctctg cggtgtgggg gccgaagccc
     6661 ttcgaaatcg tcagcaggta ttctgccgcc tgctccacgc tgtcccagcg cagcggcaga
     6721 cccagatagg cgccgatgcg ggcgatggcc ttggcctcga gcaccggccc tacatcgttg
     6781 agcacgagac ggctgacggg cgacgccgcc aatgcagcca gtcccaggcc gatcaagccg
     6841 cccatcgaag tgcccaccca atgcagggtc tcggcatgga cgcgcgccag gaccgtgacc
     6901 atgtcggcga catagcgggg aatgccgtac tccatcgggt tcgtcagccg gtccgattcg
     6961 ccgcggccgg cgatgtcggg acagacgacg cgatagtggt cactcatcgc tgcggccagc
     7021 acatcgaagt cgcggccttg tcgggtcaaa ccgtggacgc acaccagcac ccgcggattg
     7081 gatggatcgc cccattccca gtaggcggtt cggtgcaacc cctgggtatc gaggcaggga
     7141 acgtgatgca ggcgcggctt gttcatcgaa acatcgtatc aatgcgccgg gccgcctcgc
     7201 cggcatcggg aatcacttca gcatctgaac ggtcccgctg acgctcaccg aaacgctggt
     7261 ctgccccgct tccaccggca gcggcgcgtc ggcggtgagc gccatcgacc tcgcgctggc
     7321 cagcatcggc accggcgcgc cgccctggtc gttgctgccg accgtgacct cgcggatcac
     7381 gtagctcgaa taaccaaact gctgagtcac ctgagcggcc tgcgcgcgat acctgctgat
     7441 cgcctgcgcc atcacgctgc cctcggcctt ctcgcgttgc tcacgcgaca ggccctgcgt
     7501 cacccgcgcg atcgtcatcg tctggatgcg tccggccagc tgaccgatgg cctgcaggtc
     7561 gcggccttcg atcaccagtt cggccgtgcc ctgccagcca gtcagcgcgt tcttcgtcga
     7621 atagcgcgga tacatcgaga aattgcccgt tcgcacgtcg acctgacccg gctttgcaac
     7681 ggacttcgcc tcggccagcg cgccatccag cgcctgcttc agcgcagcct gcaccgcatg
     7741 cgcatcggaa ccctcgcgcg ttgcggtgag cgtgacggcc atcaggtcgc gcgtcacctc
     7801 gacgctggcc gtcgcggtca acgcaaccac gcctgtgggc accgccggct cggccgccag
     7861 cgtggtgcca gcgcagaacg ctgcagcgca gacaggcagc caggccaggc gaagacggaa
     7921 cagggacgct gggaagcgga ggaggctcat ggtgtggatt cgtgttcagc gcggactgcg
     7981 cccagtcagg ctaacgcgag tcgatcgcga atcgatgacg ccgacccgcc ctgccccacg
     8041 gccatcagat ggatatttgt aacagacagt cagaaccgac ccaaaccggc tgcgaaggcc
     8101 cgcagaatct cgcttgttac aaataggaga gtgccatgac cgctgccacc ccagcccgcg
     8161 ccgaccgtgt ggtcgtcgta gacgacgatg cccggattcg cgacctgctg cgccgctacc
     8221 tgacccagga aggtttcgaa gtcctgctgg ccgaagacgc caaggctctg aaccgggttc
     8281 tgacccgcga gaccatcgac ctgatcgtgc tcgacctgat gctgcccggc gaggacggtc
     8341 tgtcgatctg ccgccgcctg cgcgccgcca acgacctgac gcccatcatc atgctgacgg
     8401 ccaaggtcga ggatgtggac cggatcgtcg gcctcgaggt cggtgccgat gactacctcc
     8461 ccaagccgtt caacccgcgc gagttgctgg ctcgcatcca tgccgtgctg cgtcgtcgcc
     8521 cgtcgctgga agcgcccggc gcaccggcca aggaagcgct gacggtgacc ttcggtccgt
     8581 tcgagttcga tctggcgctg cggcgccttt cgaagcaggg cgagcagatg gccttgacca
     8641 ccggcgagtt ctcgatgctg aaggcgctgg ttcgccaccc gcgccaaccg ctctcgcgcg
     8701 acaagctcgc gcaactggct cgcggacgcg aattcgagcc gttcgaccgc agccttgacg
     8761 tgcagatctc gcgcctgcgc aagatgatcg agcccgatcc ggcccaaccc cgctacatcc
     8821 agaccgtgtg gggcgtgggc tacgtcttcg ttcccg
//
LOCUS       DAOORV010000136         8831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9811_length_8831_cov_6.776778, whole genome shotgun sequence.
ACCESSION   DAOORV010000136 DAOORV010000000
VERSION     DAOORV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9811_length_8831_cov_6.776778"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKV56_14680"
     CDS             <1..386
                     /locus_tag="PKV56_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine oxidase"
                     /protein_id="HOX69106.1"
                     /translation="LLHPRYPIYIAPKPGGIFVIGATEIESDDMSPVSVRSALELLSA
                     AYSVHPGFAEGRVLELCSQCRPTLHDNLPAIRWLGPRRLQINGLYRHGFLIAPAMLDV
                     AMECMSTGHSTLAERFALRVEACRG"
     gene            391..588
                     /gene="thiS"
                     /locus_tag="PKV56_14685"
     CDS             391..588
                     /gene="thiS"
                     /locus_tag="PKV56_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOX69107.1"
                     /translation="MNVLINNVAHELPAGATLADAIAALQVTPPFAAAVNMLFVPNTR
                     YQDTPLNAGDRIDVIAPVTGG"
     gene            605..1423
                     /locus_tag="PKV56_14690"
     CDS             605..1423
                     /locus_tag="PKV56_14690"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOX69108.1"
                     /translation="MNIETHPPTVPADALVLYGQTFQSRLMLGTSRYPSPQVLEDAVR
                     RARPAMLTASLRRQGALNAEAGGGFWRMLQTLGVPVLPNTAGCHGIQEVITTAQMARE
                     VFSTPWIKLELIGDDYTLQPDTLKLAEAAEWLIKDGFRVLPYCTEDLVVCQRLVDVGC
                     QAVMPWAAPIGTGKGPINPYAMQVLRERLAVPMIVDAGLGKPSHACQVMEWGYDGVLL
                     NTAVALAQDPVAMAGAFADAVQAGRSAHHAGAMGEQQSAQPSTPVLGTPFWHHS"
     gene            1420..2358
                     /locus_tag="PKV56_14695"
     CDS             1420..2358
                     /locus_tag="PKV56_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOX69109.1"
                     /translation="MNEDSIEAMAQAIITAHHARLGLPVSESAAIVFHHGDPVYRAAK
                     QAAHALGFIDADAECVALAWQSRTLRTARFDASSWPDEPVDFGLAPHPRPDAFAPCPK
                     ALGLYAVLPTAAWVGRMASAGVPTVQLRFKSDDEAAVQREVDIAVEAVRGTGSLLFIN
                     DHWQAAIRAGAYGVHLGQEDIDTADLKAIRSEGLHLGLSSHGYAEMLRVDRLSPSYVA
                     MGAVYATNLKVMPTVPQGPGRLAAYARLMRDHALVAIGGIDEQRLPDVLASGVGSVGV
                     VRALIAAEQPEAVAAKWKAVIGANDSARPVGHPQQT"
     gene            2355..3083
                     /locus_tag="PKV56_14700"
     CDS             2355..3083
                     /locus_tag="PKV56_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HOX69110.1"
                     /translation="MNTETSPDPSSNNSLQVIAFDVFGTVVDWHTGIARAVDELGLPV
                     GGDAFAQAWRDGYQPALQRVISGELEWTLLDELHRSILDGLLERFGITHLTEEQKVDL
                     NAAWCDLDAWPDVADGLTRMKEKYTICTLSNGNLGLLFHMAQHAGLPWDCILSAETFK
                     AYKPDPKTYEGVGKVLDVEPNQVLMVASHQSDLTAARACGLQTAYIERPLEWGPNRAN
                     PDRPDPANTYFAGSLTDLATQLGC"
     gene            3129..4280
                     /locus_tag="PKV56_14705"
     CDS             3129..4280
                     /locus_tag="PKV56_14705"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOX69111.1"
                     /translation="MNRPDDWQHDQEAGAARRENPLTTRDSTRVDDVRISAVRPLISP
                     ALLLDELPMPEAAQTLVRQAREASAAVLCGADDRLVAVVGPCSIHDHEQAMAYAHGLK
                     RLADELRDDLLVVMRVYFEKPRTTVGWKGYINDPRLDGSFRINEGLRRARQLLIDIAA
                     IGLPAGTEYLDLLSPQYISDLVSWGAIGARTTESQSHRQLASGLSCPVGFKNGTDGGV
                     RVAVDALVAAAAPHAFMGMTKMGQAAIFETRGNTDCHLILRGGKQPNYQADSVAAACE
                     ALRAAGLREQVMIDFSHANSEKQHRRQIDVGEDVAAQIVGGDSRICGVMIESHLEAGR
                     QDLLPGQPLKRGVSITDACLGWSDTQPLLRLLAHSVRERRTRCTELARR"
     gene            4354..4429
                     /locus_tag="PKV56_14710"
     tRNA            4354..4429
                     /locus_tag="PKV56_14710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4387..4389,aa:Asn,seq:gtt)
     gene            complement(4683..5696)
                     /gene="pbpG"
                     /locus_tag="PKV56_14715"
     CDS             complement(4683..5696)
                     /gene="pbpG"
                     /locus_tag="PKV56_14715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HOX69112.1"
                     /translation="MLGSAAPAAAATGQHAGKTSKPVLKKVSASAASSRELRRRAANS
                     PRITIRSAARAPITRTRSSVATSRLTAPRLSMGELAGLRSTADDLDLKSSVALVMDQD
                     TNEVLFSKNSQAVLPIASITKLMTAVVVTDAALPLDEVLTITQDDVDTEKGSSSRLSV
                     GSSLTREEMLHLALMSSENRAAHALGRNYPGGLTAFVTAMNQKAHQLGMTDTHYVEPT
                     GLSSQNQSSAHDLATLVKLAHTHPLIRELSTSPEFMVDIGHRQLQFRNTNGLVRNPSW
                     EIGVQKTGYIVEAGRCLVMQASLAGRKLIMVFLDSTGRNTRLADAERVRRWITAVVPK
                     AVS"
     gene            complement(5867..7408)
                     /locus_tag="PKV56_14720"
     CDS             complement(5867..7408)
                     /locus_tag="PKV56_14720"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOX69113.1"
                     /translation="MADKLVIFDTTLRDGEQSPGASMTRDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFEAVKAIAQAIKDSTVCSLARANDKDISRAAEALHGGASTRIHLFLAT
                     SALHMEKKLRMTPDQVFEQARLAVRFARNLSGDVEFSPEDGYRSDPDFLCRVLEAVIA
                     EGATTINIPDTVGYAIPELYGNFILDLRTRIPNADKAVWSVHCHNDLGMAVANSLAGV
                     KIGGARQVECTINGLGERAGNCALEEVVMAVRTRRDYFGLEVNIDASQIVPASRLVSQ
                     TTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWAANKIVLGKLSG
                     RNAFKQRLRDLGIDLESEGEINAAFAKFKELADRKSEIFDEDITALVMDESVTAENEH
                     YRLLALSQRSETGERPHASVAFAAGTAELHAESDGNGPVDASLKAIESKLGTGAEMLL
                     YSVNAITSGSTESQGEVTVRLQLGGRIVNGVGADPDIVVASAKAYLSALNKLHSTTER
                     VAAQG"
     gene            complement(7595..8437)
                     /gene="pssA"
                     /locus_tag="PKV56_14725"
     CDS             complement(7595..8437)
                     /gene="pssA"
                     /locus_tag="PKV56_14725"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOX69114.1"
                     /translation="MNLPHESGQEHPSGHEFDEGPVRPRRKGVYVLPNLFTLAALFGG
                     FYAIVMAMNGRFEQAAIGVFCAMVLDSLDGRVARMTNTQSTFGEQMDSLSDMVSFGAA
                     PALIVYEWALKGMGKLGWVAAFIYCSCAALRLARFNTNLTVVDKRYFQGLPSPAAAAV
                     VMGFIWVMDDAGYKQADMQGWLAWCAMALTVLAGLTMVTNLPFYSFKEFSLKRTVPFV
                     VIVALALAIALVNLHPPIVLFAIFCLYAGSGYGVYVWKRMKGRPVSVIATSTDEPEEE
                     GLHR"
     gene            complement(8535..>8831)
                     /locus_tag="PKV56_14730"
     CDS             complement(8535..>8831)
                     /locus_tag="PKV56_14730"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOX69115.1"
                     /translation="GGIANMNYSISNNAEYGEYVTGPKIVTDETRAAMRKALIDIQTG
                     EYAKSFILENRAGAPTLLSRRRLMAEHPIEVVGEQLRAMMPWIKKNRLVDQSKN"
BASE COUNT         1571 a         2764 c         2845 g         1651 t
ORIGIN      
        1 ggctgctgca cccccgctat ccgatctaca tcgcgccgaa gccgggcggc atcttcgtca
       61 tcggcgccac cgagatcgaa tccgacgaca tgtctcccgt cagcgtgcgc tcggcgctcg
      121 aactgctcag tgcggcgtac tcggtgcacc cgggattcgc cgaaggacgc gtgctcgagc
      181 tctgctcgca gtgccggcca acgctgcacg acaacctgcc ggcgatacgc tggctgggcc
      241 cgcgtcgcct gcagatcaat ggcctgtacc ggcatgggtt cctgattgcg ccggcgatgc
      301 tcgacgtggc gatggaatgc atgtccacag ggcacagcac gctggctgaa cgtttcgccc
      361 tgcgagtcga ggcctgccgt ggatgaagcg atgaacgtac tgatcaacaa cgtcgcgcac
      421 gagttgccgg caggtgccac gctcgccgat gccatcgcgg cactgcaggt gacgccgccg
      481 tttgccgcgg ccgtcaacat gctgttcgtg cccaacacac gctatcaaga cacgccgctg
      541 aatgcgggcg atcgcatcga tgtgatcgca ccggtcacgg gcggctgaac ccgagggccc
      601 cctgatgaac atcgagaccc atccaccgac agtcccggcc gatgcgctgg ttctatacgg
      661 ccagaccttc cagagccgcc tgatgctggg cacctcgcgc tacccctctc cgcaggtgct
      721 cgaggacgcg gtgcgccggg cccgcccggc catgctgacg gcatcgttgc ggcgccaggg
      781 cgcgctgaac gccgaggccg gaggcggctt ctggcggatg ttgcagaccc tgggcgtccc
      841 cgtgctgccc aacacggcag gctgccacgg catccaggag gtgatcacca ccgcgcagat
      901 ggcacgcgag gtgttttcga caccctggat caagctcgag ctgatcggcg acgactacac
      961 gctgcagccg gacacgctca agctggccga ggcggcggag tggctgatca aggacggctt
     1021 ccgggtgctg ccgtactgca ccgaggatct ggtggtctgt cagcgcctgg tcgacgtcgg
     1081 ttgccaggcg gtgatgccgt gggcggcgcc gatcggcacg ggcaagggcc cgatcaatcc
     1141 ctatgcgatg caggtgctgc gcgagcggct ggccgtgccc atgatcgtgg atgcaggtct
     1201 tggcaagccg tcccacgcct gccaggtcat ggagtggggc tacgacggcg tgctgctcaa
     1261 cacggcggtg gcgctcgcac aggatccagt ggcgatggct ggtgcgttcg ccgacgcggt
     1321 gcaggcaggt cgaagcgcgc atcatgcggg cgcgatgggc gagcagcagt ccgcgcagcc
     1381 gagcacgcct gtgctgggta cacctttctg gcaccactca tgaacgaaga ctccatcgag
     1441 gcgatggcgc aggccatcat caccgcccat cacgcccgct tgggcttgcc ggtctcggag
     1501 agcgcggcaa tcgtcttcca ccacggcgac ccggtttacc gtgcagccaa gcaggccgcc
     1561 cacgcgctgg gcttcatcga tgccgatgcc gaatgcgtgg ccctggcctg gcagtcgcgc
     1621 acgctgcgca cggcccggtt cgatgcatcg agctggcccg acgagccggt cgacttcggt
     1681 ctggcgcctc atcctcgccc tgacgcgttt gcgccgtgcc cgaaggcgct cggcctctat
     1741 gcggtgctgc ctacggccgc gtgggtgggc cgcatggcgt ctgccggagt gcccacggtg
     1801 cagctgcgct tcaagtccga cgacgaggcg gccgtgcagc gtgaggtgga tatcgcggtc
     1861 gaggccgtgc gcggcaccgg ttcgctgctc ttcatcaacg accactggca ggccgcgata
     1921 cgagccggtg cctacggcgt gcacctcggg caggaggaca tcgacaccgc cgacctgaag
     1981 gcgatccgca gtgaggggct gcacctgggc ctgagcagcc acggctacgc ggagatgctg
     2041 cgagtcgacc gcctgagccc gagctacgtc gcgatggggg ccgtctacgc caccaacctg
     2101 aaggtgatgc ccacggtgcc gcagggccct ggcaggctcg ctgcctatgc gcggctgatg
     2161 cgcgatcacg ctctggtggc catcggcggc atcgacgagc agcgcctgcc cgacgtgctg
     2221 gccagtggcg tcggttcggt cggtgtggtg cgagcactga tcgcggctga acagcctgag
     2281 gcagtcgcgg caaaatggaa ggcggtcatc ggcgcgaatg actccgcgcg ccccgtcgga
     2341 caccctcagc aaacatgaac accgaaactt ccccagaccc ttcctccaac aactccctcc
     2401 aggtcattgc cttcgacgtc ttcggcaccg tggtcgactg gcacaccggc atcgcgcggg
     2461 cggtcgacga actcggcctg ccggtcggcg gtgatgcctt cgcgcaggcc tggcgtgatg
     2521 gctatcagcc cgccctccag cgcgtgatct ccggcgaact ggagtggacg ctgctcgacg
     2581 aactgcaccg aagcatcctc gacggactgc tggagcgatt cggcatcacg cacctgaccg
     2641 aagagcagaa ggtcgacctg aacgctgcct ggtgcgacct cgatgcctgg cctgatgtcg
     2701 ccgacggcct gacgcggatg aaggagaagt acaccatctg cacgctttcg aacggcaacc
     2761 tcggattgct gttccacatg gctcagcacg ccggcctgcc ctgggactgc atcctctcgg
     2821 ccgaaacgtt caaggcgtac aagcctgacc cgaagaccta cgagggcgtc ggcaaggttc
     2881 tcgacgtcga accgaaccag gtgctgatgg tcgcctcgca ccagagcgac ctgaccgctg
     2941 cgcgcgcttg cggcctgcag actgcctaca tcgagcgccc gctcgagtgg ggcccgaatc
     3001 gggccaatcc cgatcgaccc gatccggcca acacctactt cgccggcagt ctcacggacc
     3061 tggctaccca actcggctgc tgagcgaccc cgccgataat cgcgcgttcc cgccatcagg
     3121 agcgcgcgat gaaccgtccc gacgactggc agcacgatca ggaagccggc gcggctcggc
     3181 gggagaaccc gctgaccacg cgcgacagca cgcgggtcga cgacgtgcgc atctccgcgg
     3241 tgcgcccgct gatttcgccg gcgctgcttc tcgacgagct gccgatgccc gaagccgcgc
     3301 agacgttggt gcggcaggcg cgggaagcct cggcggcggt gctgtgcggc gccgacgaca
     3361 ggctggtcgc ggtcgtcggc ccgtgttcga tccatgacca cgagcaggcc atggcctacg
     3421 cgcacggcct caagcgcctg gctgatgaac tgcgtgacga tctgctggtg gtgatgcggg
     3481 tgtacttcga gaagccacgc accacggtcg gctggaaggg ctacatcaac gacccgcggc
     3541 tcgacggcag tttccgcatc aacgaaggcc tgcgtcgcgc gcgccagttg ctgatcgaca
     3601 tcgcggccat cggactgcct gccggcaccg aatacctgga cttgctgagc ccgcagtaca
     3661 tctcggacct ggtgtcttgg ggtgccatcg gcgcacgaac caccgagagc cagagtcacc
     3721 gccagttggc ttcgggcctg tcgtgtccgg tcggcttcaa gaacggcacc gatggcggcg
     3781 ttcgggtggc cgtcgacgcc ttggtcgccg cagccgcccc gcatgcattc atgggcatga
     3841 cgaagatggg acaggccgcg atattcgaga cgcgcggcaa caccgattgc cacttgatcc
     3901 tgcggggcgg gaagcagccc aattaccagg cggacagtgt cgcggccgcc tgcgaggcgc
     3961 ttcgcgctgc gggtttgcgg gagcaggtga tgatcgactt ctcgcacgcc aactcggaaa
     4021 agcagcatcg gcgccagatc gacgttggcg aggatgttgc cgcgcagatc gtcggtggtg
     4081 actcgcgcat ctgcggagtg atgatcgaaa gccacctcga ggcgggccga caggatctgc
     4141 ttcccggaca gccgctgaag cgcggcgttt cgatcacgga tgcgtgtctg ggttggagcg
     4201 atacgcagcc gttgctgcgc ttgctggcgc actcagttcg ggagcgaaga acccgttgca
     4261 cggagctcgc ccggcggtga ttcagcaagc gcgccattga actgaaattg gagcttttcg
     4321 cagcggcaga aggtggctat aatcgtaggc tgttcctcga tagctcagtc ggtagagcgc
     4381 cggactgtta atccgtaggt ccctggttcg agcccaggtc gaggagccaa gatcagcaag
     4441 gccctgcacg aaagtgcggg gccttttgct tttgggttct tctgtctttg ctgcgagttg
     4501 gatatggatc tcgccttgca tcgttcggcg ctttgcggcg aggcaagagc gcagctctca
     4561 acgatgcatg gcagaccatg gcacctggcg cgcaatttca gcgcgaggtc ttttcaattg
     4621 cgcgtgccgg cccggtctgg atctggcatg tctacggcgt cagccgcaga cgatcaaacg
     4681 aatcaggaca cagccttggg gaccaccgcg gtgatccagc gacgcactcg ttccgcgtca
     4741 gcgaggcgcg tgttgcgccc ggtcgagtcg agaaacacca tgatcagctt gcgaccggcg
     4801 aggctggcct gcatgacgag gcagcgcccg gcttccacga tgtagccagt cttctgcaca
     4861 ccgatctccc agctcgggtt ccggacgagc ccgttcgtgt tgcggaactg gagttggcga
     4921 tgtccgatgt cgaccatgaa ctcgggtgag gtcgacaatt cgcgtatcaa cggatgcgtg
     4981 tgcgcgagct tgaccaacgt ggcaaggtcg tgcgcgctgg attggttctg gctcgacaag
     5041 ccggtcggtt cgacgtagtg ggtatccgtc atcccgagtt ggtgcgcctt ctgattcatc
     5101 gctgtgacga aggcagtgag accaccagga tagtttcgac ccagcgcgtg ggctgcccgg
     5161 ttctccgacg acatcagggc caggtgcagc atttcttcac gcgtgagact gctgcccacc
     5221 gacaggcgag aactgctccc tttttctgta tccacatcgt cctgagtgat ggtcagaacc
     5281 tcgtccaacg gcaatgcggc atccgtgacg acgacggctg tcatcaattt ggtgatcgat
     5341 gcaatcggca agacagcctg ggagttcttg ctgaagagta cttcgttggt gtcctgatcc
     5401 atcaccaatg caacgctgga cttgagatcc aggtcgtcgg cggtcgaacg caagcctgcg
     5461 agttcgccca tcgacaggcg cggcgccgtc aatcggcttg tggccacaga acttcgcgtt
     5521 ctggtgatgg gggctctggc cgccgagcgg atcgttatcc gcggcgaatt cgctgccctg
     5581 cgacgcaact cgcgggaaga cgctgccgac gcgctgacct tcttgagtac gggtttggac
     5641 gtcttgccgg catgctgccc tgttgcggca gctgcaggcg ctgccgagcc aagcagcaca
     5701 ggcaccgaga cgagcaatgc gagaaatagt cgggtcagag tcgtcacatg gacctcgaat
     5761 tcaacagttg atcgagtgta gagcacatga aaaaaactcg caatatcaga aacttacgtg
     5821 atatttctca agtgcttttg aatagaggtg aaggactggg agcaattcag ccctgggcgg
     5881 cgactcgttc cgtcgtgctg tgcagtttgt tgagtgccga cagataggcc ttggccgatg
     5941 caacgacgat gtcgggatct gctcccacac cgttcacgat gcgcccaccg agctgcaacc
     6001 tgaccgtgac ctctccttgc gattctgtgc tgcccgaggt gatcgcattg accgaataga
     6061 gcagcatttc tgccccggtt cccagcttgg attcgatcgc cttcaggctg gcgtctacgg
     6121 gcccgttgcc gtcgctctcg gcgtgcaact cggctgttcc agccgcgaag gcgacgcttg
     6181 catgcgggcg ttcacccgtt tcggatcgct gagacagcgc gagcagccga tagtgctcgt
     6241 tctcggctgt gaccgattcg tccatcacca gggcggtgat gtcctcatcg aagatttcgc
     6301 tcttgcgatc agccagttcc ttgaacttgg cgaacgccgc gttgatttcc ccctccgatt
     6361 caaggtcgat gccgagatcc ctcaatcgct gcttgaacgc gttgcgccct gacagttttc
     6421 ccaggacgat cttgttggcc gcccagccga catcttcggc tcgcatgatt tcataggtgt
     6481 cgcgggcttt gaggacgccg tcctggtgga tgcccgaggc atgtgcaaag gcgttggcgc
     6541 caaccacggc cttgttcggt tgaacgacaa agccggtggt ttgcgacacc agtcgtgacg
     6601 caggaacgat ctgcgacgca tcgatgttga cctccagtcc gaagtagtct cgccgtgtgc
     6661 gcacagccat gacgacctct tcgagcgcgc aatttccggc tcgctcgccc aatccgttga
     6721 tcgtgcattc gacctgcctg gcgcctccaa tcttcacgcc agccagcgaa ttggccacgg
     6781 ccattccgag gtcgttgtgg cagtgcaccg accagactgc cttgtctgca ttcgggattc
     6841 gcgtgcgcag gtcgaggatg aagttgccgt acaactcggg aatcgcataa cccacggtgt
     6901 ccgggatgtt gatcgtggtg gcaccttcgg caatcacggc ttcgagcacg cgacagagga
     6961 agtccgggtc tgaccggtag ccatcttccg gggagaactc gacgtcaccc gacaggttgc
     7021 gagcaaagcg gacggcgagc cgggcctgtt cgaacacctg gtccggtgtc atccgcagct
     7081 tcttctccat gtgcagtgca ctggtcgcaa ggaagagatg gatgcgggtc gaagcgccgc
     7141 catgcagcgc ttcagcggca cgcgaaatgt ccttgtcgtt ggcgcgcgcc agcgagcaca
     7201 ccgtgctgtc cttgatggcc tgtgcaatgg ccttgacggc ttcgaagtcc ccgtttgagg
     7261 aagccgcgaa gcctgcctcg atgacatcca ccttgaggcg ttcgagctgg cgggcgatgc
     7321 gcagtttctc gtcgcgagtc atggacgcgc cgggcgattg ctcgccatcg cgcaaggtgg
     7381 tgtcgaaaat gacgagtttg tcggccatgg tgtctttcct cggattgcgc gtgaaatggt
     7441 ctggacaaaa caaaacggcc cgctgcatct gctagcgggc cggtgagacg aatacgatgc
     7501 gacgtgaacg atcagcgagc ccgggaggtc tccagaagta gcgtcagcaa gccgtgcaag
     7561 atcgaattca tgcagtcaat gtagcacgct ggtgtcatcg gtgcaggcct tcttcttccg
     7621 gctcgtcggt tgacgtggcg atcacgctga cgggacgtcc cttcattcgc ttccagacgt
     7681 acacgccata gccggagcct gcatacaggc agaagatggc gaacagcacg atcggcggat
     7741 gcaggttgac aagtgcgatc gccaaggcca gcgccacgat gacgacgaat ggaacggtgc
     7801 gcttgaggct gaattccttg aagctgtaga agggcaggtt ggtcaccatc gtcaagccgg
     7861 ccagcaccgt cagagccatc gcgcaccagg ccagccaccc ctgcatgtcg gcctgcttgt
     7921 atccggcatc gtccatcacc cagatgaagc ccatcaccac ggcagctgcc gccggactgg
     7981 gcaggccctg gaaatatcgc ttgtccacca cggtgaggtt ggtgttgaag cgcgccagtc
     8041 gcagcgcggc gcacgagcaa tagatgaagg cggcgaccca gcccagcttg cccatgccct
     8101 tgagcgccca ttcgtagacg atcagcgcag gtgccgcccc gaaagagacc atgtcggaca
     8161 ggctgtccat ctgttctccg aaggtgctct gggtgttggt catccgggcg acccggccat
     8221 cgaggctgtc cagaaccatc gcgcagaaga cgccgatcgc agcctgttcg aatcgaccat
     8281 tcatggccat cacgatggcg tagaagccgc cgaacagggc ggccagcgtg aagaggttgg
     8341 ggagtacata gacgcccttg cggcgcgggc gcacgggacc ttcatcgaac tcgtgtccgg
     8401 aagggtgttc ttgtccactc tcgtgaggga gattcatgcc gggcaggata acgcagaggg
     8461 ccaatgaaaa agccgcgagg tgaccctcgc ggctttgctc gtcagatggt cgattggaac
     8521 ctgctgggtc gctctcagtt cttcgactga tcgaccagcc ggttcttctt gatccacggc
     8581 atcatcgcgc gcaattgctc gccgacgact tcgattggat gctcggccat caggcggcgg
     8641 cgggacaaca gcgtcggtgc gccggcgcgg ttttcgagga taaagctctt ggcgtattcg
     8701 ccggtctgga tgtcgatcag tgccttgcgc atggcggcac gggtttcgtc ggtgacgatc
     8761 tttggtccgg tcacgtactc gccgtattcg gcgttgttcg agatcgagta gttcatgttg
     8821 gcgatgccgc c
//
LOCUS       DAOORV010000137         8768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9903_length_8768_cov_10.502812, whole genome shotgun sequence.
ACCESSION   DAOORV010000137 DAOORV010000000
VERSION     DAOORV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9903_length_8768_cov_10.502812"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKV56_14735"
     CDS             <1..567
                     /locus_tag="PKV56_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOX69116.1"
                     /translation="KVSLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQILI
                     SKAMAFANNVYVAVSNAAGFDGVYSYFGHSALVGFDGRTLGECGEEEMGVQYAALSKS
                     LIRDFRRNGQSENHLFKLLHRGYTGVINSRDGDKGVAACPYSFYAKWIADPDAARAQV
                     ESFTRTTVGTDECPIEGLPNQSTVGIHR"
     gene            607..1383
                     /locus_tag="PKV56_14740"
     CDS             607..1383
                     /locus_tag="PKV56_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOX69117.1"
                     /translation="MTQEPWYQPIGDECTLFEAAYARRLPVLLKGPTGCGKTRFVEHM
                     ARRLGRPLVTIACHDDLSASDLTGRWLLDAGGTRWLDGPLTTAVRTGAICYLDELVEA
                     RSDTTVVIHPLADSRRVLPLDKCNQLVHAHPDFALVVSYNPGYASHHKVLKPSTLQRF
                     CGIEFGPPEAEIEARIVAHESGIDPALAARLVALGARTRRLQGQGLDEGASTRMLVHA
                     GVLMQQGVAAQAACRMALVTPLTDDPDLAQALRAVVDATF"
     gene            1400..3256
                     /locus_tag="PKV56_14745"
     CDS             1400..3256
                     /locus_tag="PKV56_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69118.1"
                     /translation="MSPMPDRTDSTPGPAARDWCSLRAHLQATARLLWDVAPPLHAED
                     TAESVRLSRHGVTLPLRLDAAADPGERRDCRAAIAHAGAHLVHSTQRFDPQGLKPITM
                     ALIGLLEDARVEWLACRELPGLRRLWAPFHEIDLGEAADFESLMRRLSRALLLPEAHD
                     PHPWVAKGRRLFFGSTMQGCADPAASTPSVVDCPPGLIGDPASLRRVASLLGNDIGQM
                     RLSFNAKTYRVQPTYRDDNQWLWQPSGAVTGLDSTALELPAQAHDGVSVAASSASPGG
                     KQASDPPASTAIEAHRLDRPIAQADPQADGGSRQSTELQTPVDVTVHLYPEWDRLICR
                     SRAQWCSVLESGAAAARLASHSSADGFCDSRAVEVLRMRARSLRAWVPQWAEQAARRS
                     VRQTDGDSLDIDAMVECGVALRVGQRPDERVYQGRERRRRDSVTVCLLDLSASTARSA
                     ARHTDSGLDQARIVALAEAWSEGSPARGDRSACAVHGFWSDGRHRVHYQRIKEIDEPV
                     DARVLDRLLRLRSEGSTRLGAALRHASALLLARRTPRMNLLVISDGETHDIDVHDPRY
                     LSEDARQAVREARAQGVAVRCLALDPAREEDVRAIFGRDGYRLLPLSGLRSV"
     gene            3337..6948
                     /locus_tag="PKV56_14750"
     CDS             3337..6948
                     /locus_tag="PKV56_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69119.1"
                     /translation="MAIAESSRATLAPAQPPVLGPGIVAPSDRVRGEAVQRVVKVRRD
                     YNSWVARESIEDYALRFTPRSFRKWSELRVANTAFGAASFLVLEAVGATLLVEYGFIN
                     AFWAILATGLIIFLAGLPISHYAARYGVDMDLLTRGAGFGYIGSTVTSLIYASFTFIF
                     FALEAAIMAYALELAFDIPPAWGYLICALAVIPLVIYGVTAISRLQVWTQPLWLLLMI
                     VPYIYVFHRNPGLVDDLTRYAGANGQGGQFDLALFGAAMTVGIALITQMGEQADYLRF
                     MPERTEANRRRWWAGLLLGGPGWVVLGVLKMLGGALLAYLAISHAVPVARAVDPNQMY
                     LVAYEEVFTNLVPYPLTWAIAATALLVVVSQMKINVTNAYAGSLAWSNFFARLTHSHP
                     GRVVWMVFNTVIALMLMELNVFQALGQVLGLYSNIAIAWMMAVVADLVINKPLGLSPK
                     GIEFKRAHLYDINPVGVGAMGLASVLSIAAYLGLMGSMAQAFSALIALVTAFVTAPLI
                     AWYTKGKYYLARAPLPVDPATPVDPAKPVHQAAPPGGRSEATGGPTTTCTVCEREYEG
                     EDMAYCPAYLGSICSLCCSLDARCNDMCKPQASWSAQWQSVGRAMLPSRLWVRLDSEL
                     GRYLLLMSALVPLLGLLFWMIYRQELTMQDVPDAKLSAALGMGFVKAYAALVVVTAIV
                     GWWLVLTHKSREVAQEESNRQTHALNEQTGVLRDEIASHRRTDEALQEAKRLADTANQ
                     AKSRYITTISHELRTPLNSIIGYAQLLDEDDSIPAHRKQAVSVIRRGGDHLLSLIEGT
                     LDIARIESGRFALEVKPMRLRDCLQEMTSLFELQAAGKGIEFQHAIADNVPAVVRTDE
                     KRLRQILINVLGNAVKFTSQGRVIFRVSHARQMALFEIEDTGPGIPPDELGEIFEPFA
                     RGSAIGGAASGASVGSTGLGLTISKMLTDVMGGEMTVGSIAGPVPRTGTVFRIRLFLP
                     EVRAAVAGEFDAPRTQRIGYAGSRRRLLVVDNEEVDRELLRNVLVPLGFELRQAANGQ
                     ECLDLLHEFSPDAILMDLAMPGIDGWETIRRIRAEELSDAPVAIVSANAFEKGVDNDV
                     GIPAEDFILKPVRKSELLDWLGRVLALQWVDAPQRPAAPIPPVPAAPMVLPSAERLRG
                     LQEMIDLGYFRGIVKQLDEIAANDPDCTPFVNHMRQLAQQFQLDAMTGVIRKAQDAA"
     gene            6938..7885
                     /locus_tag="PKV56_14755"
     CDS             6938..7885
                     /locus_tag="PKV56_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX69120.1"
                     /translation="MQPDSFSPFNRSIADVVLIVDDVPDNLSVLHDALDESGYTVLVA
                     TNGEAAIQRAAQALPDVVLLDAVMPGMDGFEVARRLKARPETAHIPIIFMTGLTDTEH
                     VVRAFEVGGVDYVTKPIRAKEVLARMSVHVQGARQARQARNALDAFGHATLVVRAADG
                     KVVWKTPLARDLLLQHFDGGELDDAVLAWVREELARRAVAGAVPGEPLKLSRQNRRLV
                     LALHEQTGEDEWLIVMRESSDQAVIDAVGAAFRLTTREAEVLYWVAKGKISRDIGDIL
                     SMSPATVKKHLEHIYEKLGVETRTAAAGLAMSRVRGLSG"
     gene            8130..>8768
                     /locus_tag="PKV56_14760"
     CDS             8130..>8768
                     /locus_tag="PKV56_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HOX69121.1"
                     /translation="MKSTRRTFNATLGSIALGVAGLAAPLFAQAADTIKVGVLHSLSG
                     TMAISETVLKDTVLMTIDEINAKGGVLGKQLEAVVVDPASNWPLFAEKAKQLISQDKV
                     AVTFGCWTSVSRKSVLPVYEELNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEY
                     LMSKDGGSAKRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHS"
BASE COUNT         1388 a         2901 c         3030 g         1449 t
ORIGIN      
        1 aaggtcagcc tgatcatctg cgacgacggc aactaccccg agatctggcg cgactgtgcg
       61 atgaaggggg ccgagctgat cgtgcgctgc cagggctaca tgtacccggc gaaggagcag
      121 cagatcctca tcagcaaggc gatggccttt gccaacaacg tctacgtggc ggtgtcgaac
      181 gcggcgggct tcgacggtgt gtattcgtac ttcggccatt cggcgctggt cggcttcgat
      241 ggccgcacgc tcggcgaatg cggtgaggag gagatgggcg tgcagtacgc cgcgctgtcg
      301 aagagcctga tccgcgactt ccgccgcaac gggcagagcg agaaccatct cttcaagctg
      361 ctgcaccgcg gttacaccgg cgtgatcaat tcgcgtgacg gcgacaaggg tgtggcggcc
      421 tgcccctaca gcttctatgc caagtggatc gccgatcccg atgcggcgcg agcgcaggtc
      481 gagtcgttca cccgcacgac ggtcggcacc gacgagtgtc cgatcgaggg cctgcccaac
      541 cagtcgaccg tcggcatcca tcgctgatcc gggcgtgagc gccgcctggg acggtccgtg
      601 ccgccgatga cgcaggagcc ctggtaccag cccatcggcg acgaatgcac gctgttcgag
      661 gctgcctacg cgcgccggct tccggtgctg ctcaagggcc cgaccggctg cggcaagacc
      721 cgcttcgtcg agcacatggc gcggcgactc ggtcgcccgc tggtcaccat cgcatgccac
      781 gacgacctga gtgccagcga cctgaccggt cgctggctgc tggacgccgg gggaactcgc
      841 tggcttgacg ggcccttgac caccgctgtc cgcaccggcg cgatctgcta cctcgatgaa
      901 ctggtcgagg ctcgatcgga caccacggtg gtgatccacc cgctggccga cagtcgccgc
      961 gtcctgccgc tcgacaaatg caaccaactc gtccacgcac accccgactt tgctctggtg
     1021 gtctcgtaca acccgggtta cgcatcgcat cacaaggtgc tgaagccttc gacactgcag
     1081 cgcttctgcg gcatcgagtt cggcccgccc gaagccgaga tcgaggcccg gatcgtcgcg
     1141 cacgagagcg ggatcgatcc ggcgctggcg gcccgcctgg tcgccctcgg ggcgcgcacg
     1201 cggcgcctgc aggggcaggg gctggatgag ggcgcatcca cccgcatgct ggtgcacgcg
     1261 ggggtgctga tgcagcaggg ggtggccgcc caggcggcct gccggatggc gctggtgacg
     1321 ccgttgaccg acgacccgga tctcgcgcag gcgctgcggg cggtggtcga cgcgactttc
     1381 tgaagcgtcc gctgcgccga tgagcccgat gcccgaccgg accgacagca cacccgggcc
     1441 ggccgcaagg gactggtgca gcctgcgggc gcatctgcag gcgacagcgc ggctgctgtg
     1501 ggatgtggct cccccgctgc atgccgagga cactgccgag tccgtgaggc tctcgcgtca
     1561 cggcgtgacg ctgccgctgc gtctcgatgc cgctgcggat cccggtgagc gcagggactg
     1621 ccgggcggcg attgcgcatg ccggggcgca tctggttcat tcgacgcagc gcttcgatcc
     1681 gcagggcctg aagccgatca cgatggcgct gatcggcctg ctcgaggacg cgcgcgtcga
     1741 atggctggcc tgccgcgagt tgccgggact gcgacggctg tgggctccgt tccacgagat
     1801 cgatctgggc gaagccgcag atttcgagtc gctgatgcgt cggctgtccc gtgcactgct
     1861 gctgcccgag gcgcacgatc cgcacccctg ggtcgcgaag gggcgcagac tgttcttcgg
     1921 ttccacgatg cagggttgcg ccgatccggc tgccagcacc ccatcggtgg tggactgccc
     1981 gccagggctg atcggcgacc cggccagcct gcgtcgcgtc gcatcgctgc tgggcaacga
     2041 catcggccag atgcgactct cgttcaacgc gaagacgtat cgggtgcagc cgacctatcg
     2101 cgacgacaac cagtggttgt ggcaaccgtc gggcgccgtg accgggctgg attcgacggc
     2161 gctggagctg cctgctcagg cgcatgacgg tgtgtcagtt gctgcgtcgt cggcttcccc
     2221 gggtgggaag caagcgagcg acccgccagc ctcgacagcc atcgaggcgc accggttgga
     2281 tcgaccgatc gcgcaggccg acccccaggc cgatggcggc agccggcagt cgaccgaact
     2341 gcaaactccc gtcgacgtga cggtgcacct ctatccggag tgggatcgtc tgatctgccg
     2401 atcgcgtgcg cagtggtgca gcgtgctcga aagcggcgcc gccgccgcgc ggcttgcatc
     2461 gcattcgtcc gcagacggct tctgcgattc ccgggcagtc gaggtgctgc gcatgcgggc
     2521 gcgatcgctg cgagcctggg tgccccaatg ggccgagcag gcagcgcggc gatcggtgcg
     2581 gcagaccgat ggtgacagcc tggacatcga tgcgatggtc gagtgcggtg tggctttgcg
     2641 cgtcggtcag cgcccagacg agcgggtcta ccagggccgc gagcgccgtc gccgagacag
     2701 cgtcaccgtg tgcctgctgg atctctctgc atcgacagcc cggtctgccg cacggcacac
     2761 ggacagcggg ctcgaccagg ctcgcatcgt cgcgctggcc gaggcgtggt ccgagggatc
     2821 gcctgcccgc ggcgaccgct cggcgtgcgc ggtgcatggc ttctggtccg acgggcgcca
     2881 ccgggtgcat taccagcgca tcaaggaaat cgacgaacct gtcgatgcgc gggtgctcga
     2941 tcgcctgctg cggttgcgca gcgaggggtc cacccggctc ggcgccgcgc tgcgccatgc
     3001 cagtgcgttg ctgctggccc gcaggacgcc gcgcatgaac ctgctggtga tcagcgacgg
     3061 cgaaacgcac gacatcgatg tccatgatcc gaggtacctg agcgaagacg cccggcaggc
     3121 cgtgcgcgag gcccgcgcgc agggcgtggc ggtgcgctgc ctcgccctgg atccggcgcg
     3181 cgaggaggac gtgcgtgcga tcttcggccg cgatgggtac cggctgctgc cgctctcggg
     3241 gttgcggtca gtctgaggca gcgggccgca ggctcgtctg ctcgcttgca aggcgccgtg
     3301 ccgaagactg gcacggggct tgccgcagaa ttccgcatgg cgatcgccga atcttcccga
     3361 gcaacccttg cgccggccca gcctcctgtg ctcggtccgg gcatcgttgc gccgtcggac
     3421 cgcgtgcgcg gcgaagccgt gcagcgcgtg gtcaaggttc gccgtgacta caacagctgg
     3481 gtggcacgcg aatcgatcga ggactacgcg ctgcgtttca cgccgcgcag ctttcgcaag
     3541 tggtcggaac tgcgcgtggc caacaccgcg ttcggtgccg cctcgttcct ggtgctcgag
     3601 gcggtcggcg cgacgctgct ggtcgagtac ggattcatca acgccttctg ggcgatcctg
     3661 gcgaccgggc tgatcatctt cctcgccggc ctgccgatca gccactacgc tgcgcgctac
     3721 ggcgtcgaca tggatctgct gacccgcggc gccggcttcg gctacatcgg ctcgacggtc
     3781 acctcgctga tctacgcgag cttcaccttc atcttcttcg cgctcgaggc ggccatcatg
     3841 gcctacgcgc tcgagctggc cttcgacatc ccgccggcct ggggctacct gatctgcgcg
     3901 ctggcggtga tcccgctggt gatctacggc gtcacggcga tcagccgcct gcaggtgtgg
     3961 acccagccgc tgtggctgct gctgatgatc gtgccctaca tctatgtgtt ccaccgcaac
     4021 ccggggttgg tcgatgacct gacgcgatat gccggcgcga acgggcaggg cgggcagttc
     4081 gacctcgcct tgttcggcgc ggcgatgacg gtgggcatcg cgctgatcac gcagatgggc
     4141 gagcaggccg actacctgcg gttcatgccg gagcgcaccg aggccaatcg ccgccgctgg
     4201 tgggccgggc tgttgctcgg cggtcccggc tgggtggtgc tgggcgtgct gaagatgctg
     4261 ggcggcgcgc tgctggcgta cctggcgatc agccacgcgg tgccggtcgc gcgcgcggtc
     4321 gacccgaacc agatgtacct ggtggcgtac gaggaggtgt tcacgaacct cgtgccgtat
     4381 ccgctcacct gggccattgc cgcgacggcg ctgctggtcg tggtgtcaca gatgaagatc
     4441 aacgtgacca atgcctacgc cggctcgctg gcctggtcga acttcttcgc acggctgacc
     4501 cacagccacc cggggcgcgt ggtgtggatg gtgttcaaca ccgtgatcgc cttgatgctg
     4561 atggagctga atgtgttcca ggcgctcggc caagtgctgg gtctgtactc gaacatcgcg
     4621 atcgcctgga tgatggcggt ggtcgccgac ctggtcatca acaagccgct ggggctgagc
     4681 ccgaagggca tcgagttcaa gcgcgcgcac ctgtacgaca tcaacccggt cggcgtcggc
     4741 gcgatggggc tggcatcggt gctgtcgatt gccgcttacc tcgggctgat ggggtcgatg
     4801 gcgcaggcgt tctcggcgct gatcgcgctg gtcaccgcct tcgtcaccgc gccgctgatc
     4861 gcctggtaca caaagggcaa gtactacctg gcccgagccc cgctccccgt cgatccagcc
     4921 acgcccgtgg atccggctaa gccggtccac caggcggcgc cccccggggg caggagcgaa
     4981 gcgactgggg ggccaacaac cacctgcacc gtctgcgagc gcgagtacga aggcgaggac
     5041 atggcctact gcccggccta cctcggatcg atctgttcgc tgtgctgttc gctcgacgcg
     5101 cgctgcaacg acatgtgcaa gccgcaggcg agctggtcgg cgcagtggca gtccgtcggc
     5161 cgggcgatgc tgccatcgag gctctgggtt cggctcgact ccgagctcgg tcgctacctg
     5221 ctgctgatgt cggcgctcgt tccgctgctc gggctgctgt tctggatgat ctaccggcag
     5281 gaactgacga tgcaggatgt gcccgatgcg aagctgtctg ccgcgctcgg catgggcttc
     5341 gtcaaggcct atgccgccct ggtggtggtc acggccatcg tcggctggtg gctggtgctg
     5401 acccacaaga gccgcgaggt cgcgcaggag gaaagcaacc gccagacgca tgcgctcaac
     5461 gagcagaccg gggtgctgcg cgacgagatc gcctcgcacc gccgaaccga tgaggcgctg
     5521 caggaagcca agcggctggc cgacaccgcc aaccaggcca agagccgcta catcacgacc
     5581 atcagccacg agctgcgcac gccgctcaac agcatcatcg gctacgccca gcttctcgac
     5641 gaggacgact cgatcccggc ccaccgcaag caggcggtca gcgtgatccg gcgcggcggc
     5701 gatcacctgc tgtcgctgat cgagggcacg ctggacatcg cccgcatcga gagcgggcgc
     5761 ttcgcgctcg aggtcaagcc gatgcggctg cgcgactgcc tccaggaaat gacgtcgctg
     5821 ttcgaactgc aggccgcggg caagggcatc gagttccagc acgcgatcgc cgacaacgtg
     5881 ccggcggtcg tgcgcaccga cgaaaagcgg ctgcggcaga tcctgatcaa cgtgctgggc
     5941 aatgcggtca agttcacgtc gcagggccgc gtgatcttcc gggtcagcca tgcgcggcag
     6001 atggcgctgt tcgagatcga ggacaccggc cctggcatcc cgcccgacga gctcggcgag
     6061 atcttcgagc cgttcgctcg cggatcggcc atcggcggag cagcttccgg cgcctccgtc
     6121 ggcagcaccg gtctggggct caccatcagc aagatgctga ccgatgtgat gggcggcgag
     6181 atgacggtcg gcagcatcgc cgggccggtg ccgcgcacgg gtacggtttt ccggatccgg
     6241 ctcttcctgc cggaggttcg ggcggcggtg gctggcgagt tcgacgcgcc gcgcacccag
     6301 cgcatcggct atgccggttc gcgccggcgg ctgctggtgg tcgacaacga ggaggtcgac
     6361 cgcgagttgc tgcgcaacgt gctggtgccg ctgggcttcg aactgcgtca ggccgcgaac
     6421 ggccaggagt gcctggatct gctgcacgag ttcagcccgg acgcgatcct gatggatctg
     6481 gcgatgcccg gcatcgacgg ctgggagacc atccgccgca tccgggccga agagctcagc
     6541 gacgcaccgg tggcgatcgt ctcggccaac gcgttcgaga agggcgtcga caacgacgtg
     6601 ggcatcccgg ctgaggactt catcctgaag ccggtgcgca agtcggagct gctcgactgg
     6661 ctgggccgcg tgctggccct gcagtgggtc gatgcaccgc agcgtccggc tgcgcccatc
     6721 ccgcccgtgc cagccgctcc gatggtgctg ccgtcggccg agcggcttcg aggattgcag
     6781 gagatgatcg acctgggcta cttccgcggc atcgtcaagc agctcgacga gatcgcagcc
     6841 aacgacccag actgcacccc gttcgtcaac cacatgcgtc agctcgcgca gcagttccag
     6901 ctcgatgcga tgaccggcgt gatacgcaag gcgcaggatg cagcctgact cgttctcccc
     6961 attcaaccgc agcatcgccg acgtggtgct gatcgtcgat gacgtgcccg acaacctgtc
     7021 ggtgctgcac gatgcgctcg acgaatccgg ctacaccgtg ctggtcgcca ccaacggcga
     7081 ggcggccatc cagcgcgcgg cacaggcgtt gcctgatgtc gttctgctcg atgcggtgat
     7141 gcccggcatg gacggattcg aggtcgcgcg gcgactgaag gcgcggccgg agacggcaca
     7201 catcccgatc atcttcatga ccggcctgac cgataccgag cacgtggtcc gcgcgttcga
     7261 ggtcggcggg gtcgattacg tgaccaagcc gatccgtgcc aaggaagtgc tggctcgcat
     7321 gtcggttcac gtgcagggcg cgcgccaggc ccgtcaggca cgcaatgcgc tggacgcgtt
     7381 cggtcacgcc acgctggtgg tgcgcgccgc cgacggcaag gtggtctgga agacgccgct
     7441 cgcgcgcgat ctgctgctgc agcatttcga cggcggcgaa ctcgacgacg ccgtgctggc
     7501 ctgggtgcgc gaagagctgg cgcgccgggc cgtcgcaggt gccgtccccg gcgaaccatt
     7561 gaagctgtcc cgtcagaacc gacgcctggt gctggcgctg cacgagcaga ccggcgagga
     7621 cgaatggctg atcgtgatgc gcgaaagctc cgatcaggcg gtgatcgatg cggtcggtgc
     7681 cgccttcagg ctgaccacgc gcgaggccga ggtgctgtac tgggtcgcca agggcaagat
     7741 cagccgcgat atcggcgaca tcctcagcat gagcccggcg acggtcaaga agcacctcga
     7801 gcacatctac gagaagctgg gggttgagac gcgcaccgcg gcggcaggtc tggcgatgag
     7861 cagggtcagg gggctgtcgg ggtgattccg accgtgcggt tctccgaact tgcactttga
     7921 cggagaaccc ggcctgggga tggactgcgc cgtgccgaat cccggcacag cccatcgcga
     7981 gacagtcggg gccaggagcg catgcagtcc cagcgaccga tgcggcgaac ccgactacgc
     8041 catccgtcgt atggaccctc ggccccggcc tccttaacct gcgtgagcgg ttccatccca
     8101 caccttccat attcccagga gacccctgaa tgaaatcgac acgtcgtact ttcaacgcca
     8161 cgctcggcag catcgccctc ggtgtcgccg gtctggccgc tcccctcttc gcgcaggccg
     8221 cagacacgat caaggtcggc gtgctgcact cgctgtccgg cacgatggcg atctcggaga
     8281 cggtgttgaa ggacaccgtg ctgatgacca tcgacgagat caacgcgaag ggtggcgtgc
     8341 tcggcaagca gctcgaggcc gtggtcgtcg atccggcttc gaactggccg ctgttcgccg
     8401 agaaggccaa gcagctgatc agccaggaca aggtggccgt gaccttcggc tgctggacct
     8461 cggtcagccg caagagcgtg ctgccggtct acgaagagct gaacagcctg ctgttctacc
     8521 ccgtgcagta cgagggtgaa gagctgagca agaacgtgtt ctacaccggt gccgcgccca
     8581 accagcaggc catcccggcg gtggaatacc tgatgagcaa ggacggcggc agcgccaagc
     8641 gcttcgtgct gctgggcacc gactatgtgt acccgcgcac caccaacaag atcctgcgcg
     8701 ccttcctgaa gagcaagggc gtggccgatg ccgacatcat ggaggactac acccccttcg
     8761 gccattcg
//
LOCUS       DAOORV010000138         8689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_9998_length_8689_cov_9.470118, whole genome shotgun sequence.
ACCESSION   DAOORV010000138 DAOORV010000000
VERSION     DAOORV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9998_length_8689_cov_9.470118"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(594..929)
                     /locus_tag="PKV56_14765"
                     /pseudo
     CDS             complement(594..929)
                     /locus_tag="PKV56_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1338
                     /locus_tag="PKV56_14770"
                     /pseudo
     CDS             1069..1338
                     /locus_tag="PKV56_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
     gene            1664..6526
                     /locus_tag="PKV56_14775"
     CDS             1664..6526
                     /locus_tag="PKV56_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOX69122.1"
                     /translation="MRIHYLSAQGVLEVEQGSLRELERSLPREWMGFAAFRLLARGSK
                     LPLDVDLLVLTSNRILVVELKNWAGDIEYSAGQWIHKGTPKRSPVEVNDNKARVLKGF
                     IKDKDSALTVPFIENVVVLCHPQCRLVRFPDEEKRFVMTLADFCHAASDPTRYKARFP
                     ETPFGQPYRSRDPLPDRQKYERIFSARSEGILQRRMVLHGFEQADATADYDHPKKIWT
                     EFRAEHRENRGSKALLRKWNFQELAGGGTTPVERQTIGLRELRLNEMLRVQAPELHAD
                     LLEPVAPATADDVTTNFVEAYRLPASIERLAEHLARQQSMSIDDRRALALSILARFAK
                     LHTLGIAHRDITKKTLWVMEPARVILSTFAAARVPEALTVGAHRFELETGSIDLPEDD
                     GAEKLKSTSSPFARDVFLIGVLVYEMLEAKELERVNRVPLYDTKLALQLPALGPWYER
                     AMDWDPSARFQSAVEALDALNDCLAVERGPTVSEADFEAYRTEASPFTLPMKESISSA
                     GGKMVYLSERGGAKVLVKCWTNLKYDPKYGTRNLRLLGFLQRARSLRQSGFDAAPEVL
                     DFGIGQFGLILVTRWVEGSTLATWLATEPSARRRAVVALSLFNAVCRLHALGLSHGDL
                     KAENIVVAKADENEGERAVLLDVPDLSADGDEGMTVGSVPPALESASPKHRDLYAVTN
                     IGLGLLSEEFPNSRRDAERALDLGEVLPPLELLAETLKGELTPPVDTTPTYLVLLRRR
                     GRDVPAPRDLDSNNGEFSVGVVRDEHAGEQRFILTGVRQQLFIKFDLIRRIAVDANLK
                     EIGHQEFVSAYKRRSFALRAKVRVAWHDAADATALGEDLYALYLDSLPEGGAQEVGWR
                     RPAVDPYAEGAARAGELRGVSAGELWTALADTDDMNGASITVRAGARQVPEGSGSWLI
                     PFDLDGGVLDFSEDERIELLERGYDQIDGGERWYTVGIVAPDIGKDVIRVQQTSLRFG
                     PKQGMSLFLRGALERSASERRVAAMNRVLAGGALIPRLVDYFDPDTELTPYRAEVGDL
                     GDLSEYELNDQQAEALRTAVSFGPVSLLQGPPGTGKTKFIASFVNLVLSRGLASNVLL
                     VSQSHEAVNNAMEKVVELSKKSGMDDTMVRIGLPSMVSPQLRPIQEDALRQRYRETFD
                     AELKQRVRAVGYAMGLPQEYVRAAVEMHTSLGSLLERITHIERAQGTTEDGPEGSSTH
                     VQRLREVFVEIASARFGIEASSADDLEAVLQAELVDLAALHDSPSPVKCERLGQIARL
                     STEFSQVLRNPRSNFTAFLSRSASVVAGTCVGVGKHALGIVDLAYDWVIVDEAARASP
                     MELVVAMQAGKRVLLVGDHLQLPPIYPVAVEENVAQLLGISRGDFRRINNFQRAFSSN
                     YGKAVGRTLLRQYRMASAINQVVSHCFYKDALEVGREAPGPEYDELPDYLAKQVVWVD
                     TCDQARSAFHRSAGTHEGALENEAEAVAVVEIVRSIAKSGDFLRRVRDLDGGKHTPIG
                     IIAMYAAQRDLIRRKLDQADWAADIRDLYSVGTVDSYQGKENRIIILSVVRNDTSKTI
                     GFLSDPERINVAMSRAKDRLVVVSSSAMWQLHPKSPMHSVLEEVRKLAAIDDAAFVPS
                     QDLKRSFAHA"
     gene            6519..8072
                     /locus_tag="PKV56_14780"
     CDS             6519..8072
                     /locus_tag="PKV56_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69123.1"
                     /translation="MRERFNTVAVAIPAQQFHVRCHVSIERQVPVMTDFAVRLLHLTG
                     PLEIGAMQEYFGLSGNELVDLLDLLRSEGLVEESGGRFSLTSYAEGRFVGSSDGLPRF
                     TRIVERQSRPVFELLSYAPIPKALSGTYWDNALELDWASDDEAHGQTIERAEAAFHQH
                     FLDIERFQQDEEWRRAYSCYKVDSITSGRPFNVPLPVHFDVDLDGNVEFDVDVQLDLL
                     PDELRSRVRQLTADRIGSLKAHGDHFKGFVEFFDDALLSRYLLDVNAERPGAVVQSGH
                     KLVLRKAPDFDFQRYVREVHAGADGEIYDDRQSRALLGALYMPKNQDRLIETLSAAAE
                     HYKKTATADRRFPREILWVIPDTALWGRTELVKQLVERLSATVAKAWGEPVEVVGIAP
                     APQDEPVDRIRKRGNLLLDAGFSEVFLGPAIAMFERFEAMVLPGVYGAAMYQWKVPSM
                     DVLSVPVGFGTRSPTTLRRLSTLARNTGASALHRVRRGSGKDGEDKKLKYAASSAAHF
                     LYLDAFIAE"
     gene            complement(8391..>8689)
                     /locus_tag="PKV56_14785"
     CDS             complement(8391..>8689)
                     /locus_tag="PKV56_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69124.1"
                     /translation="SRDCSPEAYIRRIRRLIEDDAPPARGCWYPSVRVVEAHPLAVHA
                     KQLDLTIEREQRYGEWFARVALSNERRNLIFDFADMFPDLRGWQLAAVSGLPAS"
BASE COUNT         1630 a         2640 c         2770 g         1649 t
ORIGIN      
        1 agcggctcga gcccgatccg caaaatccca ggatcatcaa gacggtctac ggcgctggtt
       61 acatgctttc ggccgcagtc gagtggtcct gaagcgaaat ggcggagctg gccagacagt
      121 cgaggttagt cggtatcagc ctggagcgac aacggacgga tctgacaacc atcgagcggt
      181 tccaagcata cagttgactg ctgtgccgta cgactgtcaa cgtcgggttt tggagctcgc
      241 cgccattcgg cagggaaacg cggccgacag ctgttagtct tgaagaatcg atctcgtcga
      301 aacggccaag gtccgttgat ggccgctcag cgacgcacgc gactcgcgcc cgactgttca
      361 cttcaatctg atcgcgaacc ttcagcaagc tgatcccagc ttcagctctg cggtatcgag
      421 cgacatccgg cctgcccagg tgggcttacc ctcgaagccg atgaggaaca tccgcgagcg
      481 tcggcagtgg ttggcgagag ctaaccgctg gagccacaga cgctgctttc ggaagcatga
      541 ggagcgccgt acccatggca gcaaggcgac gcagcgccgg agacttgctc gcactacgct
      601 ccggctactt cagcgaccag ggcggtgtcc ccgcccgctc gcgcatcgac ctgcatggca
      661 tccaggtcga tgattgcatc ggccatcgct gcgaggtgcg gggtctgggt gatgaagaac
      721 tcctgccggt agccgccgag ccgaaggact tcgcgcttca tggccatgaa catgcgcttg
      781 tgttctgggt cgaggggccc atcggcctcg tcggagaaga gcgtgtcgaa tcgccgtccg
      841 gtgttctggg ccaagtacag ggcgatcgcg cgcgtcaggc aggcgtcgat gaacgttcgc
      901 tcaccgccgc tcatcaggct gaggctcttg cctccgcgcg cgttggcgct gatcgctgcc
      961 ggccccgacg tgttatgccg catcgcttcg caggtacccg cagcacagtt gcaggggctg
     1021 gcacgcaggc cagaatgcga actcggcata tccgggctct cggcatagtg gatcaacgag
     1081 tgcctgaccc gggcgatcgc gctctacttg tccggcaaca ccggccgccg gatcggcacg
     1141 ctgttcagcg acgaggcgga cggaccgctg gacccggagc acaagcgcat gttcatggcg
     1201 atgaaacgcg aggtgctgcg ccttggcggg tacgcaagcg agttcttcgt gagccagaca
     1261 cccgaactga ccgcgatggc cgatcgcatc atcgacttgg aaagcttcgc tgcagtgggt
     1321 gcgcgggcgg tcgcatgact gaaaaagtcc gcgtgctacc ccgtcgtact ccttggacca
     1381 ccgaccggtg atgcgtgcca gtgaacgccg cgtagccgca gcgaggccgt ctcttgtacg
     1441 gtctcattgg gcggcgctgg acggccacat gcggccgttc aactcttgaa agctggtgac
     1501 cgtgcaggac attttgtgcc ggtcgacctc tgcctgctct gtgccggctc tcgcttgaga
     1561 gtaggcatca aatcaggcat ctccatgcat acttgcatga gatgcggtgc cccacgtagt
     1621 cgccgcttcc ttcatcgatc ggttgggaag ggagttcaac agagtgcgaa tccactacct
     1681 gagtgcacag ggtgttcttg aagtagagca aggctcgttg cgtgaattgg agcgcagcct
     1741 gccgcgcgag tggatgggct tcgcggcgtt tcgactcctt gctcgaggct cgaagctccc
     1801 attggacgtg gatcttctgg tcctgacatc gaatcgcatt cttgtcgttg agttgaagaa
     1861 ctgggccggt gacatcgagt actccgctgg tcaatggatc cacaaaggaa cgccgaagag
     1921 gtcgccggtc gaagtcaacg acaacaaggc ccgagtcctc aagggcttta tcaaggacaa
     1981 ggactccgcg ctgacggttc cgttcatcga aaacgttgtt gtcctgtgtc acccgcaatg
     2041 ccgcctcgtg cgattccccg acgaggaaaa gcgctttgtt atgacgctcg cggacttctg
     2101 tcatgcagcg tcggacccca cgcggtacaa ggcgaggttc cctgaaaccc ctttcggaca
     2161 accgtatcgg tcgagggacc ccttgcctga tcgtcagaag tacgagcgga tcttctccgc
     2221 tcggagcgag ggcattcttc aacgtcgcat ggtcctacac ggctttgaac aggccgacgc
     2281 gaccgcggac tacgaccatc cgaagaagat ctggaccgag ttccgcgccg agcacagaga
     2341 aaaccgcggc tctaaggcgc tgctgcgcaa gtggaacttt caggaactgg ctggtggcgg
     2401 caccacgccc gtcgagcgac aaacgatcgg gctgcgcgaa ctgcgcctga atgagatgct
     2461 gcgcgtgcag gcgcctgaac ttcacgccga tcttctggag cccgtcgccc cggccaccgc
     2521 tgacgatgtc actacgaact tcgtcgaagc ctaccggttg cccgccagca tcgagcgact
     2581 tgctgagcac ctcgcgcgcc agcagagcat gtcaatcgat gaccgccgcg cgctggcatt
     2641 gagcatcttg gcgcggttcg cgaagctcca cacgctcggg atcgcccata gggacatcac
     2701 caagaagacc ctctgggtca tggagccggc tcgcgtcatt ctttcgacgt tcgccgctgc
     2761 tcgcgttcct gaggcactga ctgttggcgc acatcgcttc gagctggaga ccggctcgat
     2821 tgatcttcct gaggacgacg gcgccgagaa gctgaagtca accagcagtc ccttcgcccg
     2881 cgatgtcttc ttgatcggtg tcctcgtgta cgagatgctg gaagccaagg agttggaacg
     2941 ggtcaatcgc gttccgctct acgacaccaa gctggcgctt caactacctg cccttggtcc
     3001 ctggtacgag cgagcgatgg actgggatcc atcggcacgg tttcagtcgg ctgtcgaggc
     3061 gcttgacgcg ctgaacgact gccttgcagt cgagcgcggg ccaaccgttt ccgaagcgga
     3121 ctttgaggca tatcgcaccg aggccagtcc gttcacgttg ccaatgaagg agtcaatctc
     3181 atcggccggc ggcaagatgg tctacctgtc cgagcgcggc ggggccaagg tcttggtcaa
     3241 gtgctggacg aatctcaagt acgaccccaa gtacggaacc cgcaacctgc ggctactcgg
     3301 gttcttgcaa cgagcgaggt cgctgcggca atctggcttc gacgccgctc ccgaagtcct
     3361 ggacttcggc attgggcagt ttggcctgat cctcgtcacc cgttgggtcg agggcagcac
     3421 cttggcgaca tggcttgcta ccgagcccag cgcacgccgg cgcgcggtgg tcgccctgtc
     3481 gctgttcaac gcagtgtgca gactccatgc gctgggcttg agccatggtg acttgaaggc
     3541 cgagaacatc gttgtcgcca aggccgatga aaatgaaggc gaacgcgccg tcttgctcga
     3601 tgtgccggat ctgagcgccg acggcgatga ggggatgaca gtcggcagcg tgccgcccgc
     3661 cttggaatcg gcttcaccga agcatcgcga cctctacgcc gtaaccaaca ttggccttgg
     3721 tctcctgagc gaggagtttc cgaattctcg aagggacgct gagcgtgcgc tcgaccttgg
     3781 cgaggtgttg cctccgcttg agctcctggc tgagacgctg aagggcgagt tgacacctcc
     3841 ggtcgacacg acgcccacat atctggttct gcttaggcgg cgaggccggg acgtgcccgc
     3901 ccctcgcgac ctggatagca acaatggcga gttctcagtc ggggtggtgc gcgacgagca
     3961 tgctggcgag cagcgattca tcctgacagg cgtcaggcaa cagctcttca tcaagttcga
     4021 cctgataaga cggattgccg tcgacgcaaa cttgaaggag atcggtcacc aggagttcgt
     4081 cagcgcgtac aagcgacgta gctttgcatt gcgcgcgaag gttcgcgttg catggcacga
     4141 cgcggccgat gccacggcgt tgggtgaaga cctgtacgcg ctttacctcg acagcttgcc
     4201 ggaaggcggt gcgcaagagg tcggctggcg ccggcctgct gtcgatccct acgccgaagg
     4261 tgcggcgcgc gcgggtgaat tgcgaggcgt ctccgctggc gagctttgga cggcgctggc
     4321 ggacaccgac gacatgaacg gcgcgtccat caccgttcgt gccggtgccc gacaggtgcc
     4381 ggaaggaagc gggagttggc tcattccctt cgacctcgat ggcggcgttc tcgacttttc
     4441 tgaagacgag cgcatcgagt tgctcgagcg cggctacgac cagatcgacg gtggggagcg
     4501 ctggtacacg gtgggcatcg ttgcgccaga catcggaaag gacgtcatcc gcgtccagca
     4561 gaccagtctt cgcttcggcc ccaaacaggg catgtcgctg tttctgcgcg gtgcgctgga
     4621 gcggtcggct tcggagcgcc gagtggctgc gatgaacaga gttctggcgg gcggcgcact
     4681 catcccaagg ctcgttgact acttcgatcc cgacacggag ctgactccat accgggcaga
     4741 ggtgggagac ttgggcgact tgtctgagta cgagttgaac gaccagcagg ccgaagcgct
     4801 gcgtacagcc gtctcgtttg gaccggtgtc gctgctacag ggaccgccgg gcactggaaa
     4861 gacgaaattc atcgcgtcct tcgtgaacct cgtgctctcg cgcggcttag ccagcaatgt
     4921 cttgctggtc agccagtcac acgaagccgt caacaacgcg atggagaagg tcgttgagct
     4981 gagcaagaag agtggcatgg acgatacgat ggttcgtatc ggtctgccgt caatggtttc
     5041 gccgcagctc aggcccatcc aggaggatgc gctacgacag cgctatcgcg agaccttcga
     5101 cgccgagttg aagcagcgtg ttcgcgctgt gggctatgca atgggcctgc cgcaagagta
     5161 cgtccgggcc gcggtggaaa tgcacacgag ccttgggtca ctgctggagc gcattactca
     5221 tatcgagcgc gcgcaaggca ctaccgaaga tgggcctgaa ggctcctcga cgcacgtaca
     5281 acgcctgcgc gaagttttcg tggagatcgc atcagctcga ttcggcatcg aggcctcatc
     5341 ggctgacgat cttgaggccg tgctgcaggc ggaactggtc gatttggctg ctctgcacga
     5401 ttcgccgagc ccggtcaagt gcgagcggct cggccagatt gcaaggctgt ccacggagtt
     5461 ttcccaggtc ctgcgtaatc ctcgctccaa cttcactgct ttcctgtcgc gcagcgccag
     5521 tgtcgttgcc ggcacctgcg tgggcgtcgg caagcatgcg ctgggcattg tggacctagc
     5581 ctatgactgg gtcatcgtgg acgaggccgc acgggcatct cccatggaac tggtcgtcgc
     5641 aatgcaggct ggaaagcggg tgctgctggt cggcgatcac ctgcagctgc cgccgatcta
     5701 tcccgtcgcc gtggaagaga acgtcgcgca gcttctgggc atctcgcgcg gtgatttccg
     5761 gcggataaac aacttccaac gagcgttttc gtcgaactac ggcaaggcag tcggccgcac
     5821 gcttctgcgc cagtaccgca tggcctctgc tatcaatcag gttgtgtcgc actgcttcta
     5881 caaggatgcc cttgaggttg gcagagaggc gcccggcccc gagtacgatg aactgccgga
     5941 ctacctggcc aagcaagtcg tctgggtgga cacgtgcgat caggcgcgca gtgccttcca
     6001 ccgcagcgca ggtacgcacg aaggcgcact ggagaacgaa gccgaggccg tcgcggtcgt
     6061 cgagatcgtg agaagcatcg ccaagtcggg cgacttcctt cgacgggttc gcgacctgga
     6121 cggaggcaag cacacgccga tcgggatcat tgccatgtac gcggcgcagc gagacctcat
     6181 tcgccgcaag ctggaccagg ccgactgggc tgcggatatc cgtgacctgt acagcgttgg
     6241 aacggttgac agctatcaag gcaaggagaa ccgaatcatc atcttgtccg tggtgcgcaa
     6301 cgacacctcg aagacgatcg gctttctctc cgaccccgag cgcatcaacg tcgcgatgtc
     6361 gcgagcgaag gaccggctcg tcgtggtcag ctcatcggcc atgtggcagt tgcatcccaa
     6421 gtcgcccatg cacagcgtgc tcgaggaagt caggaaactc gctgccatcg acgacgcggc
     6481 tttcgtgcct tcgcaggact tgaagcggag cttcgcccat gcgtgaacgc ttcaacactg
     6541 tcgcagtcgc catcccggcg caacagtttc acgtgcgttg ccatgtgtcg atcgaacggc
     6601 aggtgccggt gatgacggat ttcgcagtcc ggctactgca cctcactggg ccgctggaga
     6661 tcggcgccat gcaggagtac ttcggcctca gcggcaacga actagtcgac ctgctcgacc
     6721 tgcttcgcag cgaggggctc gtcgaggagt ccggtggcag gttctcgctc acatcgtacg
     6781 ccgagggccg cttcgttggc tccagcgatg gcctgccgcg cttcaccagg atcgtcgaac
     6841 gccagagccg gccggtcttc gagctactct cgtatgcgcc catcccgaag gcgctatcgg
     6901 gaacgtactg ggacaacgcg ctcgaactcg actgggcttc ggacgacgag gcgcatgggc
     6961 agacgattga gcgcgcggag gcggccttcc atcagcactt ccttgacatc gagcgctttc
     7021 agcaagacga agagtggcgc cgcgcgtact cttgctacaa ggtcgacagc atcacatccg
     7081 ggcggccctt caacgtccca cttcctgtcc actttgatgt ggatctcgac gggaacgtcg
     7141 agttcgacgt cgacgttcag cttgacctac ttccagacga actacgttcg cgggtccggc
     7201 aactcaccgc cgacaggatc gggagcctca aggcgcacgg cgaccacttc aagggcttcg
     7261 tggagttctt cgatgacgcg cttctgtcgc ggtacttgct agacgtgaat gctgaaagac
     7321 caggcgcagt cgttcaatca gggcacaagc tggttctgcg caaggccccg gacttcgact
     7381 tccaacgtta cgtccgcgaa gtccatgccg gcgctgacgg ggagatctat gacgacaggc
     7441 agagccgcgc tctgcttggc gcgctttaca tgcccaagaa ccaggatcgc ctgatcgaga
     7501 cactctcggc agcggcggaa cactacaaga agaccgccac tgccgacagg aggttcccgc
     7561 gtgagatcct ttgggttatt cctgacacgg ccttgtgggg ccgcacggaa ctggtcaagc
     7621 aacttgtcga gcgcctgagc gcgacagttg ccaaggcatg gggcgagccc gtcgaggtgg
     7681 tggggattgc cccggcgccc caagacgagc cagtagatcg catacgcaag cgcggcaacc
     7741 ttctgctgga cgctggcttc agcgaagttt tccttggccc ggccatcgcc atgttcgagc
     7801 gctttgaagc gatggtcttg cccggcgtgt acggcgctgc catgtaccaa tggaaggtgc
     7861 cttccatgga cgtgctgagc gtgccggtgg gcttcgggac gcgctcacca acgacacttc
     7921 gccgcctttc gacacttgcg cgcaataccg gggcgagcgc gcttcatcgc gtccgaaggg
     7981 gctcaggaaa ggatggcgag gacaagaagc tgaagtacgc ggcgtcatca gctgcgcact
     8041 ttctgtatct ggacgccttc atcgccgagt aggtgacgac ttttgatctc gtgacgggtc
     8101 gacatgtagc gaagacctgt ctctagcatt cttgcagtga ccgaccattc gcggcataac
     8161 ttgtggtcac tgatccggca gagcgaatga ccgagacggg tcgtccacgg ccggccgcct
     8221 cggcggcacg ggtgtctggt catcgctgga cagcgggcgc cggcgcctga cgcggagtgc
     8281 atcggtagtg ggcctctgca acgagccggc agggcctctc tggaggcctc catgggtcgg
     8341 gggctccaga ggggcccctg agcaactgct caggggcgag agaatcgacc tcagctcgcc
     8401 ggcagaccgc tgacggctgc cagttgccag ccgcgcaggt cagggaacat gtcggcgaag
     8461 tcgaagatca ggttgcgccg ctcgttggag agcgccaccc gcgcgaacca ttcgccatac
     8521 cgctgctcgc gctcgatcgt gagatccaat tgcttggcgt ggaccgcgag cgggtgcgcc
     8581 tcgacaacgc gaacggacgg gtaccagcag cctcgcgccg gcggcgcatc gtcctcgatg
     8641 aggcggcgaa tgcgccggat gtacgcctcc ggtgagcagt cccgcgagc
//
LOCUS       DAOORV010000139         8676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10021_length_8676_cov_5.667208, whole genome shotgun sequence.
ACCESSION   DAOORV010000139 DAOORV010000000
VERSION     DAOORV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10021_length_8676_cov_5.667208"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..664
                     /gene="alkB"
                     /locus_tag="PKV56_14790"
     CDS             23..664
                     /gene="alkB"
                     /locus_tag="PKV56_14790"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HOX69125.1"
                     /translation="MSLFEDDSAPRIEPLVPGAVVWRAGARASDSALLTAVQEVAAQA
                     PWRHLITPGGFRMSVAMTSCGPLGWVSDRRGYRYESIDPDSGRAWPSMPHAFQALAKV
                     AASQAGFDSFEPDACLINRYVPGARLTLHQDLDERDAHSPIVSVSLGLPAIFQFGGAA
                     RSDPKQRITLDHGDVVVWGGPARFFFHGVLPLKDGCHPLTGAQRINLTFRRAR"
     gene            complement(691..1620)
                     /gene="rfaE1"
                     /locus_tag="PKV56_14795"
     CDS             complement(691..1620)
                     /gene="rfaE1"
                     /locus_tag="PKV56_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOX69126.1"
                     /translation="MIPTPEELAQRRVLVVGDVMLDRYWHGAVDRISPEAPVPVVRVS
                     REEERLGGAANVALNIKTLGARVTLLTVVGCDEAASRLRVLLEQQGIETRLGEDARLR
                     TIVKLRVIGRAQQLLRIDFENEPDHEVLEQMLDDFERELPMHDVVLFSDYGKGGLTHI
                     TRMIETARAAGKPVLVDPKGSDYTRYAGATVITPNRGELAQVVGAWSSEAQLVERAQA
                     LRDRLRVDALLLTRSEDGMTLFDDGGSVNEPAQTREVFDVTGAGDTVIAAMTALMAAG
                     LTAREAMPHANRAGGIVVGKFGTASVSYAELFG"
     gene            2006..2371
                     /locus_tag="PKV56_14800"
     CDS             2006..2371
                     /locus_tag="PKV56_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOX69127.1"
                     /translation="MIRTLVTLLVAAFAFAAHAAVDVNKASQAELETIKGIGPNMSGK
                     ILDERKKGSFKDWSDVVSRVKGVGPGNATRFSADGLTVNGSAFKSELVASSKSPAAAD
                     RKNAPAPEAPLQPAASPRK"
     gene            2458..2865
                     /locus_tag="PKV56_14805"
     CDS             2458..2865
                     /locus_tag="PKV56_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOX69128.1"
                     /translation="MPKLVTDPCPCGAAQRYADCCGRYHAGPEQLRAPDAESLMRARY
                     CAYVLQLGEYLLATWHPSTRPASPMKFEAGLRWLGLQVLRHEAVDADRARVEFVARSK
                     LGGRAHRHHELSRFVREDGRWLYVDAVNDAGLR"
     gene            2881..2956
                     /locus_tag="PKV56_14810"
     tRNA            2881..2956
                     /locus_tag="PKV56_14810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2914..2916,aa:Ala,seq:cgc)
     gene            complement(3184..3945)
                     /locus_tag="PKV56_14815"
     CDS             complement(3184..3945)
                     /locus_tag="PKV56_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69129.1"
                     /translation="MQSDLYRKQYDFELEQRNSIASATNIPLVAITVVAGAVSAVLLE
                     YRYEQCLTTWAFAISISAALASVCFSVFSIFRSFWNYEYKKLPSSALLKKFQGDLLKW
                     HIENGTNEAEAKKYADDDFLDYLTDRLADATDWNGQNNIVRGNYLHRATAAIALGVAF
                     LLPAALLYAYSKATAEEEIHQVHLIGPSTTAPEEINMSSTKPPASSHQPASAPAPAPA
                     SAPVPSAKPLGPPNTLFKGNTDLVKPSAKTSLPKK"
     gene            complement(4188..6542)
                     /locus_tag="PKV56_14820"
     CDS             complement(4188..6542)
                     /locus_tag="PKV56_14820"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOX69130.1"
                     /translation="MNEAETRAEHIDPALKAAGWGVVEGSRVLREHGITLGRLQGSGG
                     QSRRAKAEIADYVLVYRNTKLAVIEAKAWAKPLTEGVGQAKSYATKLAVRFTYATNGQ
                     AIYGIDMTTGAEGDVAGYPSPDELWALTFAESDAATAAWRERFAAVPFEDKGGTWQGR
                     YYQDIAITRVLEAIAAGRDRILLTLATGTGKTFIAFQLAWKLFQSRWNVADWKSGAEP
                     TRRPRILFLADRNILADQAYNAFSAFPDDALVRIDPADIRKKGRVPKNGSIFFTIFQT
                     FMSGPGGAPYFGDYPADFFDFIVIDECHRGGASDESNWRAILDYFAPAVQLGLTATPK
                     RTLNGDTYAYFGEPVYVYSLKEGINDGFLTPFKVKQIATTLDEYVYTPDDQVVEGEIT
                     TGKRYEEKDFNRVIEIKEREEYRVKLLMQMIDQREKTIVFCATQVHALAVRDLINQLK
                     TSTNPNYCARVTADDGAIGDQHLRDFQDNEKTIPTVLTTSQKLSTGVDARNVRNIVLM
                     RPVNSMIEFKQIIGRGTRLYDGKDYFTIYDFVKAHHHFSDPEWDGEPIEPEAKTVSTG
                     SGGTEVNTKEPPPETPPEVRAARIKVKLADGKARSIQSMTATTFWSADGRPMSAAQFL
                     EVLFGALPEFFKDEDELRRIWGAPDTRKALLAGLADKGFGREPLAEMQKIIEAENSDL
                     FDVLAYVAFAAEPVTRAERAIAAKSATAAEFTDKQQAFVDFVLAQHVRQGVDELDAEK
                     LSPLLKLKYNNAIADAFAELGQPEQVRKVFVGFQRHLYELRSNG"
     gene            complement(6554..7702)
                     /locus_tag="PKV56_14825"
     CDS             complement(6554..7702)
                     /locus_tag="PKV56_14825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOX69131.1"
                     /translation="MTGDAAFRRLADVVVAKAGNNKIIKGTQSDRPRSGLVQGFSASG
                     PDVWVATADYTRPGVVLSAVGARCGKTFLAEGAWTAIANTHVLLPSPLVDHRWLWYLT
                     NDESFWVRGGSAQPFVKVKDTLQKEVKVPPLREQQRIVAILDEAFEGIATAKANAEKN
                     QQNACDLLDNHLKCTFRTREEMGAEVQIGSVCQRLHQGLNTAGVKIKFQETGYPIIQT
                     RNIEDGIINLDEKIKFVSKEDWNFYKEKFRPEIGDVFFTNIGTIGKTAIIAEDCEYLI
                     HWNIFKLRPKFKSISSAYLKYALENLTKSGYFKSMQKGGTVDFITKAMISEAHIFLPS
                     IIRQAEITAELDSLREETQRLESNYQSKLGALADLKNSLLSLAFTGAL"
     gene            complement(7699..>8676)
                     /locus_tag="PKV56_14830"
     CDS             complement(7699..>8676)
                     /locus_tag="PKV56_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX69132.1"
                     /translation="HLYEAKIKNMGNAGRNGGEYYTPRPLIRAMIKVLDPQIGDRIYD
                     GACGSAGFLCEAFDHLLPQATKADDLKTLQTRTFYGKEKKSLAYVIAIMNMILHGIEA
                     PNVVHTNTLGENINDIQDRDRYDVILANPPFGGKERKEVQQNFPIKTGETAFLFLQHF
                     IKSLKAGGRAAIVIKNTFLSNTDNASTSLRKLLLESCNLHTVLDMPGGTFQGAGVKTV
                     VLFFEKGSPTHGVWYYQLNPGRNMGKTNPLNDDDLADFIEQQKTFADSSQSWSVDVDD
                     IDAATFDLSVKNPNGGDEAALRTPQEIMDEIAALDAESAEVLATIRELL"
BASE COUNT         1518 a         2725 c         2658 g         1775 t
ORIGIN      
        1 acgggtcggc gtcgccgcca atctgtcgct gttcgaggat gacagcgccc cccgaatcga
       61 acccctggtg cccggggcgg tggtctggcg cgccggagca cgggcgagcg attcggcgtt
      121 gctgaccgcc gtgcaagaag tcgccgcaca ggccccctgg cggcacctga tcacgccggg
      181 cggctttcgc atgtcggtcg cgatgacgag ttgcggaccg ctgggctggg tcagcgatcg
      241 gcgcggctac cgctacgaat cgatcgaccc cgacagcggc cgtgcctggc cgtcaatgcc
      301 acacgcattc caggcgctgg cgaaagtcgc cgccagccag gccgggttcg acagcttcga
      361 gcccgatgcc tgcctgatca accgctacgt gcctggcgcc cgtctgaccc tgcaccagga
      421 tctcgacgag cgagacgccc actcaccgat cgtctcggtg tcgctcgggc tgccggcgat
      481 cttccagttc ggcggtgcgg cgcgcagcga ccccaagcag cgcatcacgc tcgatcacgg
      541 cgatgttgtc gtgtggggcg ggccggcgcg cttcttcttc catggcgtgc tgccgctgaa
      601 ggacggctgc catccgctga ctggtgcgca gcgcatcaac ctgacgttcc gccgggcacg
      661 ctgaaccggc gggcgcgccg gcaaggcagc tcagccgaag agctcggcgt agctgacgct
      721 cgcggtgccg aacttgccga cgacgatgcc gccggcgcga ttcgcgtgcg gcatcgcttc
      781 gcgcgcggtc aggcctgcgg ccatcagcgc ggtcatcgca gcgatcacgg tgtcgcccgc
      841 gccggtgacg tcgaacacct cacgcgtctg cgccggctca ttgaccgacc caccgtcgtc
      901 gaacagcgtc atgccgtctt cggatcgggt cagcagcagc gcatccaccc gcaaacgatc
      961 tcgcagcgcc tgggcgcgtt cgacgagttg tgcttcgctg ctccaggcac cgaccacctg
     1021 cgcgagctcg ccgcggttcg gcgtgatcac cgtggcgccg gcatagcgcg tgtagtcgct
     1081 gcccttcgga tcgaccagca ccggcttgcc ggcggctcgg gcggtctcga tcatccgcgt
     1141 gatgtgtgtc aggccaccct tgccgtaatc ggagaacaga acgacgtcgt gcatcggcag
     1201 ctcgcgctcg aaatcatcga gcatctgttc gagcacctcg tgatccggct cgttctcgaa
     1261 gtcgatgcgc aacagctgct gtgcccggcc gatgacgcgc agcttgacga tggttcgcaa
     1321 ccgggcatcc tcgcccagcc gggtttcgat gccctgctgc tccagcagga cccgcagacg
     1381 cgaggcggct tcgtcgcagc cgaccacggt cagcagcgtc acgcgtgcac ccagcgtctt
     1441 gatgttgagc gcaacgttgg ctgcgccacc gagtcgctct tcttcgcgcg agacgcgaac
     1501 caccggcaca ggcgcctccg gcgagatgcg atcgaccgcg ccgtgccagt agcgatcgag
     1561 catcacgtca ccgaccacca gcacacgacg ctgcgcgagc tcctccggag tcggtatcac
     1621 gacgcggccc ccgcagcgtc agccgggaag gccaggccgt gtgcatccgg ggtgctgggg
     1681 aagatcaggc gggggacggc gacaaggctc atggtgtgcg cggggaacgg tgcagctccg
     1741 gtttatatca agtgcgccat ccggctcgac acctcaggcg gatgcggtgc gtctcggccc
     1801 cccccgacac ctccgacccg cttcggaggg gggcctcgat ccacgacgaa cgcaggcagc
     1861 gcaagaggag tcggggaggc ccgcacccgg ccgagtgccc tcgcgctggg gacagccaaa
     1921 caacccgccg atctccagaa tttcttccag gtcgaagcca gcgcgctgat gaccggtcac
     1981 ctcaacccaa ccactggaga acgccatgat ccgcaccctc gtcaccctgc tcgtcgctgc
     2041 cttcgccttt gccgctcatg cggcggtcga tgtcaacaag gcatcgcagg ccgaactcga
     2101 gaccatcaag ggcatcggcc ccaacatgtc gggcaagatc ctcgacgagc gcaagaaagg
     2161 ctcgttcaag gactggtccg atgtggtgag ccgcgtcaag ggcgtcggcc ccggcaacgc
     2221 cacgcgcttc tctgccgatg gactgaccgt caacggctcg gcattcaagt cggaattggt
     2281 ggcttcgagc aagtcgcccg cagccgctga ccgcaagaac gccccggcac ccgaggcgcc
     2341 gctgcagccc gcggcctcgc caaggaagtg aggtcgggcc catcggatca acgccccgcc
     2401 ctggcggggc gttgaaaatc accaaccacc cggcatctcc ccaccgcaac gcccgccatg
     2461 cccaagcttg tgaccgatcc ctgcccctgc ggtgccgccc agcgctacgc cgattgctgc
     2521 ggccgatacc acgccggccc cgaacagttg cgggcccccg atgccgaatc gctgatgcgc
     2581 gcccgttact gcgcctacgt gctgcaactc ggcgaatacc tgttggccac gtggcacccg
     2641 agcacccggc ccgcgtcacc gatgaaattc gaagccgggc tgcgctggct cggtcttcag
     2701 gtgctgcgac acgaggcggt cgatgccgac cgcgcgcggg tcgaattcgt ggctcggagc
     2761 aagctcggcg gacgggccca tcgccatcac gagctcagcc gattcgtgcg cgaggacgga
     2821 cggtggctgt atgtcgatgc cgtcaacgac gcgggcttgc gctagactcc tcacgcgtgt
     2881 gggggggtag ctcagctggg agagcgccgc gttcgcaatg cggaggtcgg gagttcgatc
     2941 ctcctcctct ccaccataca gctaggaaac acgggccttc gcggcccttt ttcgtttata
     3001 cccatcaaac ggcccatcaa tttggcaagt gcttgatttg ggttggtcag atccgcgctt
     3061 gttggccact cggacgagtg ctcggagaag actgtcacgc cgcagagctg ttggcctcta
     3121 gcaccatgcg ctatgactac cggtaggaac aggaagtgtt agccggcact ggcgagggcc
     3181 agcctatttc ttcggcaagc tcgttttggc gcttggcttg accagatcgg tgtttccttt
     3241 gaacagcgta ttgggcggac ccaaaggctt agcacttggc acaggtgccg aagccggcgc
     3301 tggcgctggt gcggaagccg gctgatgcga actggcaggt ggtttggttg atgacatgtt
     3361 gatttcctct ggagcggtag tgcttggtcc aatgagatgg acttgatgga tctcttcttc
     3421 tgcggtggcc ttgctgtatg cgtaaagcag ggcggccggt aaaagaaatg ctacgcccaa
     3481 ggcaatggca gcggtcgcac ggtgcaggta gttcccccgt acgatgttgt tttgaccgtt
     3541 ccagtcagtt gcatcagcca gacggtcggt gaggtaatcc aagaagtcgt cgtctgcata
     3601 tttcttggct tcagcctcat tcgttccatt ctcgatgtgc cacttgagaa gatccccttg
     3661 gaacttcttc aacagggccg aacttggtag cttcttgtac tcatagttcc aaaaagagcg
     3721 gaagatgctg aaaacggaga agcagactga cgccaaggct gccgatattg aaattgcgaa
     3781 tgcccaggtg gttaggcatt gttcgtagcg atattcaagc aagactgctg aaacagcacc
     3841 tgcaacaacg gtgatcgcaa ccaacggaat attcgtggcc gacgcgatcg aatttcgttg
     3901 ttccaactca aagtcgtact gcttacggta tagatcgctc tgcatactct gttcggccta
     3961 acgttgaaga gcagacattc gccgaaggag agtctgcagg aactgcggct taggcttcgg
     4021 cacgattggc cagcatgtga ctgccaagtt ccaggtggat aaccctgcgc tttgtcacat
     4081 tgaatttgct gcaaatctca tcaggtgacc atacgtggaa cagaggcgaa tccaactcca
     4141 ctgtgagctc atcggcctca tcttgggcca gaacaaaaat gaccggctta gccatttgag
     4201 cgcagctcgt acagatgccg ctggaacccc acgaacacct tgcgcacctg ctccggctgg
     4261 cccagttcgg cgaacgcatc ggcgatcgcg ttgttgtact tgagcttgag cagcggtgac
     4321 agcttctcgg cgtccagttc atcgacaccc tgcctgacgt gctgcgccag cacgaagtcg
     4381 acgaacgcct gctgcttgtc ggtgaactcg gcggccgtgg ctgacttggc tgcgatggca
     4441 cgctcagccc gcgtcaccgg ctcggccgcg aacgccacat aagccagcac gtcgaacaga
     4501 tcgctgttct cggcctcgat gatcttctgc atctcggcca gcggctcgcg gccgaagccc
     4561 ttgtcggcca gaccagccag cagcgccttg cgggtgtccg gcgcacccca aatgcgccgc
     4621 agctcgtcct cgtccttgaa gaactcgggc agcgcgccga acaacacttc caggaactgc
     4681 gccgccgaca tcggccgccc gtcggcgctc caaaaggtcg tggccgtcat gctctgaatg
     4741 ctgcgggcct tgccgtcggc cagcttgacc ttgatccgag cggcacgcac ctcgggcggc
     4801 gtttcgggtg gcggttcctt cgtgttgacc tcggtgccgc ctgacccggt actcacggtc
     4861 ttggcctctg gctcgatcgg ctcgccgtcc cactcggggt cgctgaaatg gtggtgcgcc
     4921 ttcacgaagt cgtagatcgt gaagtagtcc ttgccgtcgt acagccgggt gccgcgaccg
     4981 atgatctgct tgaactcgat catcgaattg accggccgca tcagcacgat gttgcgcacg
     5041 ttgcgggcgt ccacgccggt gctgagcttc tgcgacgtgg tcagcaccgt cgggatcgtc
     5101 ttctcgttgt cctggaagtc gcgcaggtgc tggtcgccga tcgcgccgtc gtccgccgtc
     5161 acgcgggcac agtagttcgg gttggtgctg gtcttgagct ggttgatcag gtcgcgcact
     5221 gccagcgcat ggacctgggt ggcgcagaag acgatcgtct tctcgcgttg gtcgatcatc
     5281 tgcatcagca gtttgacgcg gtactcctcg cgctccttga tctcgatgac acggttgaag
     5341 tccttctctt cgtaccgctt gccggtcgtg atctcgcctt cgaccacctg gtcgtcgggc
     5401 gtgtagacgt actcgtccag cgtggtggcg atctgcttga ccttgaacgg cgtcaggaag
     5461 ccgtcgttga tgccctcctt cagcgagtac acgtacaccg gctcgccgaa gtaggcgtag
     5521 gtgtcgccgt tgagcgtgcg cttcggcgtg gccgtcaggc cgagctgcac cgccggcgcg
     5581 aagtagtcga ggatcgcccg ccagttgctc tcgtcgctgg cgccgccgcg gtggcattcg
     5641 tcgatgacga tgaagtcgaa gaagtcagcc gggtagtcgc cgaagtacgg cgcgcccccg
     5701 ggcccgctca tgaaggtctg gaagatcgtg aagaagatgc tgccgttctt cggcacgcgg
     5761 cccttcttgc ggatgtcggc cgggtcgatg cgcaccagcg catcgtcggg gaaagccgag
     5821 aaggcgttgt acgcctggtc ggccaggatg ttgcgatcgg ccaggaacag gatgcgaggc
     5881 cggcgggtgg gttcggcccc gctcttccag tcggccacgt tccagcggct ctggaacagc
     5941 ttccacgcga gctggaaggc gatgaaggtc ttgccggtgc cggtggccag cgtcagcagg
     6001 atgcgatcgc gcccggcagc aatcgcttcc agaacgcggg tgatcgcgat gtcctggtag
     6061 tagcgcccct gccaggtgcc gcccttgtcc tcgaacggga cggcggcgaa acgctcgcgc
     6121 caggcggcgg tggccgcgtc cgattcggcg aaggtcagcg cccacaactc gtcggggctc
     6181 gggtagccag cgacgtcgcc ctcggcaccc gtggtcatgt cgatgccgta gatcgcctga
     6241 ccgttggtgg cgtaggtgaa gcgcacggcc agcttcgtgg cgtagctctt ggcctggccg
     6301 acgccttcgg tgagcggctt ggcccacgcc ttggcctcga tcaccgccag cttggtgttg
     6361 cggtacacga gcacgtaatc ggcgatctcg gccttggctc gtcgtgactg gccgccgctg
     6421 ccctgcaagc ggcccagtgt gatgccgtgc tcgcgcagta cgcggctgcc ttcgaccaca
     6481 ccccagccgg cggccttcag cgcagggtcg atgtgctcgg cgcgggtttc ggcttcgttc
     6541 acgagctgcc cttctacaac gcgcccgtaa aggccaggct gagaagggaa ttctttaggt
     6601 cggccagggc accgagctta ctctgatagt tggactccaa acgttgagtt tcttcccgaa
     6661 gtgaatccag ctctgccgta atttcagctt ggcgaattat tgatggcaaa aaaatatgtg
     6721 cctcagaaat catcgcttta gtgatgaaat caacagtccc gcccttctgc atactcttaa
     6781 aataaccgct ctttgtgaga ttctcaaggg catattttaa atacgccgac gaaatcgatt
     6841 taaatttagg ccttaatttg aaaatgttcc agtgaattag gtattcgcaa tcctctgcaa
     6901 tgattgcagt cttaccaatt gttccaatat ttgtaaaaaa tacatcgcca atttcagggc
     6961 gaaatttctc tttgtaaaaa ttccaatctt ctttggagac aaatttaatc ttctcatcga
     7021 gattgattat tccgtcttcg atatttctag tttgaattat tggataaccc gtctcttgaa
     7081 atttaatttt tacccccgcg gtatttagtc cttgatggag gcgttggcaa acacttccaa
     7141 tctgcacttc cgcacccatt tcctcccgtg ttctaaaagt gcatttcaag tggttatcta
     7201 acaaatcaca cgcgttctgc tggttcttct cagcgttggc cttggcggtg gcgatgccct
     7261 cgaaggcttc gtcgaggatg gcgacgatgc gctgttgttc gcgcagcggt ggaaccttta
     7321 cttccttttg caaggtgtcc ttaaccttca caaatggttg cgcggatcca ccccgcaccc
     7381 aaaaggactc gtcattggtc aagtaccaaa gccaccgatg gtcgactagc ggggacggca
     7441 gcagtacgtg cgtgtttgca attgcagtcc acgcgccctc tgcaagaaag gtcttgccac
     7501 atcgcgcacc gacagcagag aggactacac ccggtcgggt gtagtctgcc gtcgctaccc
     7561 acacgtctgg cccacttgcg ctgaagccct gcaccaagcc gctcctcggt cgatcgctct
     7621 gtgtaccctt gataattttg ttgttgccgg ccttggcgac cactacatct gcgaggcggc
     7681 gaaaggccgc gtcgcccgtc ataacaactc ccgaatagtc gccagtactt ccgcgctctc
     7741 cgcatccagc gccgcaatct cgtccatgat ctcctgcggc gtccgcagcg ccgcctcgtc
     7801 cccgccgttc gggttcttga ccgacaggtc gaaggtggct gcgtcgatgt cgtccacgtc
     7861 caccgaccag ctctgcgatg aatcggcaaa cgtcttctgc tgctcgatga agtcggccag
     7921 gtcgtcgtcg ttcagcgggt tggtcttgcc catgttgcgg cccggattca actggtagta
     7981 ccagacgccg tgcgtgggcg accccttctc gaagaacagc accaccgtct tgacgcccgc
     8041 gccctggaag gtaccgccgg gcatgtcgag cacggtgtgc aggttgcagc tttcgagcag
     8101 cagcttgcgc aggctggtgc tggcgttgtc ggtgttgctc aggaaggtgt tcttgatgac
     8161 gatggccgcg cggccgccgg ctttcaggct cttgatgaag tgctgcaaaa acaggaacgc
     8221 cgtctcgccg gtcttgatcg ggaaattctg ctgaacctcc ttgcgctcct tgccgccgaa
     8281 cggcgggttg gccaggatca cgtcgtagcg gtcgcggtcc tggatgtcgt tgatgttctc
     8341 gcccagcgtg ttggtgtgca cgacgttggg cgcctcgatg ccgtgcagga tcatgttcat
     8401 gatcgcgatc acgtaggcca ggctcttctt ctccttgccg tagaaggtgc gcgtctgcag
     8461 cgtcttgagg tcgtcggcct tggtggcctg cggcagcagg tgatcgaagg cttcgcataa
     8521 aaagccggcc gagccgcagg cgccgtcgta gatgcgatcg ccgatctgcg ggtcgagcac
     8581 cttgatcatg gcgcggatca gcgggcgcgg cgtgtagtac tcgccgccgt tgcgcccggc
     8641 gttgcccatg ttcttgatct tggcttcgta caggtg
//
LOCUS       DAOORV010000140         8658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10058_length_8658_cov_10.164826, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000140 DAOORV010000000
VERSION     DAOORV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10058_length_8658_cov_10.164826"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1505
                     /locus_tag="PKV56_14835"
     CDS             264..1505
                     /locus_tag="PKV56_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOX69133.1"
                     /translation="MLTDADCRNATCPSDAKRRRLTDSGGLYLEVSPKGSKRWFWKFY
                     PNGKESRLALGSYPEVSLKAARAAREDAKKTKASGTNPVQQRKADKIASAASSAITYE
                     AVAREFHGVKASGWSEGHATKWIRMNELYLFPQIGSLALDSIKAHVLLVAVRKVEAKG
                     ILSTAQDLLAMAGQVFRYGVQSGRCERNPATDLRGALKPHVAKHFAAITDPVEAGSLL
                     RAIDGYTGQPTTRAALILASLFFQRPGNIHGMEWAWVDLEARMLTIPAESMKLSKHDK
                     LNGKPHLVPLARQAVAVLKALEPLTGHGRYVFPGARTGERPMSDNTINAALRRLDYGT
                     DDHVAHGFRAMARTMMAERLNGIDPDVVEAQLGHGKRGPLGSAYDRAEYMAQRRQIMQ
                     TWADYLDQLRATATVHPLKAA"
     gene            1851..2312
                     /locus_tag="PKV56_14840"
     CDS             1851..2312
                     /locus_tag="PKV56_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69134.1"
                     /translation="MADTKYPTRVPAPTAHEMRIDIEARGDVCWEGTIDQLRSEGLIR
                     ADVVLPQRNKLAMWEDDTFEFWMSRFRPPGMKGPMRLWVEGDWWRLHRRLLPCSPHAT
                     VPASVYAKQQALAQERRLYTSAGALDSMRQRAANDDGAFQRFKLRVMTSQA"
     gene            2870..3223
                     /locus_tag="PKV56_14845"
     CDS             2870..3223
                     /locus_tag="PKV56_14845"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOX69135.1"
                     /translation="MLHGGVDLRIFLSQIRSRYQQHEALMMQVTPKSKNSKQTAPQWG
                     HQPIEAASFDAALLKIKTVSAITGLSAPTIYRKLAAKQFPEPVRLGTRCTRWPAAGVR
                     AWLAEQASAPKAATL"
     gene            3231..4172
                     /locus_tag="PKV56_14850"
     CDS             3231..4172
                     /locus_tag="PKV56_14850"
                     /inference="COORDINATES: protein motif:HMM:NF015340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HOX69136.1"
                     /translation="MQPLRHPPPQARPPAPRLIEGPKDKAPGGQAKGFEGHITESKFD
                     CRPRTAEGQGLASGALQALPAERERAMSTYEIADLTGKRHDNVLADARKMLAELHGER
                     GVLNFQDTYINPQNSQRYPVYMLPKREALLVVSGYSLKLRVKIIDRLQELEDLHAKSA
                     ADPMALLQDPATVRALLLGYADKVIALEHAVQAIAPKADALDRIATVSEGAIPVRAAA
                     NLANVPQGQFVAFLIEQGWVYRLGGRGRLMAMADKLRAGLVEMKRVEVPGAAGEPRAV
                     AQTLITTRGLAKLAEMIERKAPHLRKPQARGAQGSLL"
     gene            4223..5584
                     /locus_tag="PKV56_14855"
     CDS             4223..5584
                     /locus_tag="PKV56_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69137.1"
                     /translation="MARLTYSRGKNLKDNRPVQCSAAEVGEFIEQLNAGRAPLKEGAA
                     YVCGPLGGDGRRCADNALPRTWVAVDLDGIEAPTLPDVIRWAERYEGCWWHTHSSTPE
                     APRMRIVFVLARPADRDECIALGVELEGEALGMFGAAVKWDRCTFRPEQAVLVPPPGA
                     HVNRMHGALLPVPAAQAAGRTKDRGAAAVAQKTGDEAGCTEEDAAEVDLHRRGRKVLK
                     PSSVSSVSSVHAGAAVVAGVVWPIPPDTIPTEAGQRNDWLFRLARHVKAIQPPPTPDE
                     LRGIVRAWHDLALPAIQTTDFAASLGEFRHAYEQVKYPHGATMDAIIASIDTTAPLPA
                     GIDRLGYGEAANQLVRVCAALQAHFGSDPFFLSAREAEKLVGTPYRDANRMMQAFVQD
                     GVLEVVKKGAGMKASRYRFVWDDTASGNSSSAPMVAAPAAPSSAGTAPAAWKIPADWQ
                     NAA"
     gene            5619..5810
                     /locus_tag="PKV56_14860"
     CDS             5619..5810
                     /locus_tag="PKV56_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69138.1"
                     /translation="MPWRLMLAAMAGDLAPPEPIQPGPAHRHREGMTCLPAPPFPFAA
                     IWCDREPVVRGHLALWADR"
     gene            5807..6841
                     /locus_tag="PKV56_14865"
     CDS             5807..6841
                     /locus_tag="PKV56_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX69139.1"
                     /translation="MNAHPRPAHTPHAGALATMGATGSRAGRADAARVMLARSLALRP
                     DHAPTLLNYGIVLHQLGRDAEALLTVERALSLNPEFARAWGCYASLLHRKVQRSGDQA
                     SLGAAAYAYRTACRLGDGDPESIAFALASLGAAEQPGIAPRAYVERLFDDYAPTFDSH
                     LCDGLGYRAPELLVNALTRTRGTAPADIADLGCGTGLCGPLLRPVARRLAGVDLSRRM
                     LDMAAQRACYDALAQCDIVDFLCSHPATFDLAVAADVLVYIGDLLPLFQAAHSALRHG
                     GWLAITVERNDGEGFGLHVSRRYTHSITYLAETAARAGFEVVALTATTVRMDAGLPVK
                     GALVLLRRSP"
     gene            complement(6867..7238)
                     /locus_tag="PKV56_14870"
     CDS             complement(6867..7238)
                     /locus_tag="PKV56_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69140.1"
                     /translation="MELVMVEGKSWWVPASRPYTLAVQIETEDEYMPAIRGKLYDLIE
                     AFGPYPEALAIVTWLMDMQGVRINAVDDPDDLIAQVLATSSIGEMVRAGAPWLSEPEP
                     RESAIEWVESQAELTLGEFLG"
     gene            7351..7992
                     /locus_tag="PKV56_14875"
     CDS             7351..7992
                     /locus_tag="PKV56_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69141.1"
                     /translation="MSKGGKRWGAGRPGWHVKAEQCLRLDVRHWQRDGLLAPGRMGEW
                     EWRDATGHKVAGVTFTVTEGAVTLTRGGRLPSGVRGNVATLGAAACPPAGNIATQVLP
                     ITRTACHFGGDRPWFACPQCHRRAAVLYLRDHFGFACRPCAQVAYASQSEDAIARAWR
                     CQGKVEGRLGAHGSKPPGMHRTTHAKLAAKISACEQRRLAALVAWLPSHRRPA"
BASE COUNT         1582 a         2898 c         2739 g         1439 t
ORIGIN      
        1 aacggcagct tcccaagctt catacgaggg ttcgattccc ttcacccgct ccagatcagt
       61 gaaccacgac agttcacaac cgctcaagcc cctaggtgaa tcaagcactt aggggctttt
      121 ttcatgtcat gttgacgcac agccagtctg gacagcgcat cggatggcgg ggaacaatgc
      181 ggggaacatg ctgcggcacc gggacacgac ccgccgcttg ttccccggta cgccccgacc
      241 accacccgac cagagacgct gccatgctga ccgacgctga ctgccgcaat gcgacctgcc
      301 caagcgatgc gaaacgccgc cgcctgaccg acagcggagg gctgtacttg gaggtttcgc
      361 ccaagggctc aaagcgctgg ttctggaagt tctaccccaa cggcaaggag tcgcggcttg
      421 cgctgggcag ctaccccgag gtctcgctga aggcggcgcg ggcggcgcgt gaagatgcca
      481 agaagaccaa ggcatcgggc accaaccctg tgcagcagcg caaggccgac aagatcgcca
      541 gcgccgccag cagcgcgatc acctacgagg cggtagcccg cgagttccac ggggtgaagg
      601 ccagcggatg gagcgaaggc cacgcgacga agtggattcg catgaacgag ttgtacctgt
      661 tcccgcagat cggatcgctg gcgctggact cgatcaaggc ccatgtcctg ctggttgccg
      721 ttcgcaaggt ggaggcgaag ggcatcctca gcacggcaca agacctgctg gcgatggctg
      781 ggcaagtctt ccgctacggc gtgcagtccg ggcggtgtga gcgcaacccg gcgaccgact
      841 tgcgtggcgc gctgaagccg catgtcgcca agcacttcgc cgccatcact gatcccgtcg
      901 aggctggctc tctgctgcgt gccatcgacg gatacacggg gcagccaacg actcgggccg
      961 cgttgatcct cgcatcgctg ttcttccagc gcccagggaa cattcacggc atggagtggg
     1021 catgggtaga ccttgaggcc aggatgctca ccatcccggc cgaaagcatg aagctgagca
     1081 agcacgacaa gctgaacggc aagccgcatc tggttcccct cgcgcgccaa gctgttgcgg
     1141 tactcaaggc attggagccg ctgacaggcc acgggcggta tgtgtttccc ggcgcccgca
     1201 ctggcgaaag gccgatgagc gacaacacca tcaatgcagc attgcggcgc ctggactacg
     1261 gcaccgacga ccacgttgcc cacggcttcc gtgcgatggc gcgcacgatg atggctgaac
     1321 gcctgaacgg cattgatccc gatgttgtgg aggcgcagtt gggtcacggc aagcgcggtc
     1381 cgctcggcag cgcctacgac cgtgccgagt acatggccca gcgccgccag atcatgcaga
     1441 cctgggccga ctacttggac cagctacgcg ccaccgccac tgtccatccg ctcaaagctg
     1501 cctgaataaa ggaataccaa ttaccttgca cttcgtctat taaacgaata cactattctt
     1561 catgccgagc aagcgaacca ccgacgaagc cagcccaagg ggcaggggcc gccccgccat
     1621 tcaggagggt gaagcaacag tgccagtcac catccgcatg actgcgccgc agcggtccaa
     1681 gctggcaagg ctgggtggcc cgcagtgggt gcgctcaaag atcgacaagg caccggagcc
     1741 gaaggattga acgccaagcc ctgcggcgac ttgcagggca aatgacgagg gccggtagcg
     1801 cgccaacgct gaccgaccct ctgaccacaa ccactcggat aggagtgaac atggctgata
     1861 cgaagtatcc cacccgcgtc cctgcgccga ccgcccatga aatgcgcatc gacatcgagg
     1921 cccgtggcga tgtctgctgg gagggcacca tcgaccaact gcgcagcgag ggactgatcc
     1981 gtgctgatgt cgtcctgccc cagcgcaaca agctggcaat gtgggaagac gacacgttcg
     2041 agttctggat gtcgcgtttc cgaccgcccg gcatgaaggg gccgatgcgc ctctgggttg
     2101 agggtgactg gtggcgcctg catcggcgct tgctgccatg ctcgccgcat gcaaccgtgc
     2161 ccgccagcgt ctatgcgaag cagcaggcac tggcccagga gcgccggctg tacaccagcg
     2221 ctggtgctct tgactcgatg cgccagcgcg ccgcaaacga cgacggcgca ttccagcgct
     2281 tcaagctcag ggtgatgact tcgcaagcct gatgcggtag tagctcatgc cctggcagag
     2341 ttgctcggcg gcatgcgcag gtcttccgtg cccatagtcc agcaggtcga tcccgaccag
     2401 ggagagggct gaagcatggg cctcactagc acttgttcca gtgagaactc tggcgccgtc
     2461 actagcactt gttccagtga cgagcggccc gcatccatcg gcgtcactag aacttgttcc
     2521 agtgagaacc gtcgcggtgg gcctgttctc actagaactt gttccagtga cattccatgt
     2581 cctatccctg tccgtcctgt gtcctccgtc ttgggcgggc gattggatgg atgggcgggg
     2641 agattggcct gccgccttgg tctgtgggtg cttgcgcagt ctgcgcgtgg cctgtctgcc
     2701 ggcatgggag ttcggctgtg agcgtcgtcg ccgcccgcgc ctgggtcttg ccgcccggct
     2761 ttagggtccc atcgaccccc cgcccccgcc ggcacacgcg caggtcagat cgggaacccg
     2821 gtgaaatggg agagcgctgg taattcctcc agcacaaccg gtcgtttggg tgcttcatgg
     2881 tggggttgac ttacggattt tcctgtctca aatccgctca cgttatcaac aacatgaggc
     2941 gctcatgatg caggttactc ccaagtcgaa gaattcgaag cagaccgccc cgcaatgggg
     3001 ccaccaaccc attgaggccg cgtcctttga cgctgcactg ctgaagatca aaaccgtcag
     3061 cgcgatcacc gggttgtcgg cgccgacgat ctatcgcaag ctggccgcaa agcagtttcc
     3121 tgagccggta cggctgggca cacggtgcac ccgatggcct gcggcgggtg tgcgtgcgtg
     3181 gcttgctgaa caggcgtcag cccccaaggc ggcaacgctg tgagcacacc atgcagccac
     3241 ttcgccaccc gccaccgcag gcccggccac cagcgccgcg cctgatcgaa ggccccaaag
     3301 acaaagcccc tggcggtcag gccaagggct tcgaggggca catcacagaa agcaaatttg
     3361 attgtcggcc acgcaccgca gaaggtcaag gtctcgcctc tggtgcactc caagcgctgc
     3421 ccgccgaacg cgagcgtgcc atgagcacct acgaaattgc cgacctaacc ggcaagcggc
     3481 atgacaacgt tttggcagac gcccgcaaga tgctggccga actgcacggc gaaaggggtg
     3541 tcctcaactt tcaggacacc tacatcaacc cgcagaacag ccagcgctac cccgtctaca
     3601 tgctgccgaa gcgcgaagcg ttgcttgtcg tctccggcta cagcttgaag ctgcgggtca
     3661 agatcatcga ccggctgcaa gaactcgaag acctgcacgc caagtcggcc gctgacccga
     3721 tggcgctgct gcaagacccg gccaccgtgc gcgcgctgct gctgggctat gccgacaagg
     3781 tgattgccct tgagcatgcc gtccaggcga tagcacccaa ggccgacgcc ctcgaccgca
     3841 ttgccaccgt gtctgagggt gcgatacccg tgcgtgctgc ggcgaacctt gccaacgtac
     3901 cccagggcca gttcgttgcc ttcctgattg agcagggctg ggtctaccgc ctgggtggac
     3961 gcggtaggct gatggccatg gctgacaagc tgcgcgctgg gttggtcgag atgaagcgcg
     4021 tagaggtgcc gggcgcagca ggcgagccta gagcagttgc gcagactctc atcaccacca
     4081 gggggcttgc caagctggca gagatgatcg agcgcaaggc gccgcatctg cgcaagcccc
     4141 aggccagggg cgcacaagga agcctgctgt gagtgccgca gccgcatcgg atgcgatggc
     4201 aggcagcacg ggggcggcga ccatggcgcg gctgacctac tcccgaggga agaacctgaa
     4261 ggacaaccgg ccagtccagt gcagcgccgc tgaagtggga gagttcatcg aacagttgaa
     4321 cgctggccgc gccccgctga aggaaggtgc cgcctacgtc tgcgggccgc tgggcggtga
     4381 cgggcggcgc tgtgcagata acgcactacc gcgcacctgg gttgcggtgg accttgacgg
     4441 gatcgaagcg cccaccctgc ccgatgtcat tcggtgggcc gagcggtacg aaggctgctg
     4501 gtggcacacg cacagcagca cccccgaggc tccgcgcatg cgcatcgtgt tcgtgctcgc
     4561 acgcccggca gaccgcgacg agtgcatcgc gcttggcgtc gaactggaag gcgaagcgct
     4621 cggcatgttc ggcgcggcag tgaagtggga ccgctgcacc ttcaggcccg aacaggcagt
     4681 gcttgtgccg ccgcccggcg cgcatgtcaa tcgcatgcac ggcgcgctgc tgcctgtccc
     4741 cgcagcgcag gctgccggcc gcacaaagga tagaggggcc gcagcagttg cacagaagac
     4801 aggcgatgaa gccggttgca cagaagagga cgccgccgaa gtggacttgc acagaagagg
     4861 ccggaaggta ctgaagccat cttctgtctc ttccgtctct tctgtgcatg cgggtgctgc
     4921 cgttgttgcc ggggttgttt ggcccatccc gcccgacacc atcccgactg aagcagggca
     4981 acgcaacgac tggctattca ggctcgcccg gcacgtcaag gccatccagc cgccacccac
     5041 gcccgatgaa ctgcgcggca tcgttcgggc ttggcatgac ctcgctctac ccgcaatcca
     5101 gaccacggac ttcgccgcat cgttgggcga gttccgccac gcctacgaac aggtgaagta
     5161 cccgcacgga gccaccatgg acgccatcat cgccagcatc gacaccaccg cccccttgcc
     5221 tgccggcatc gaccgccttg gctatggtga ggccgcaaac caactggtca gagtctgcgc
     5281 tgcgctccaa gcgcacttcg gcagcgaccc cttctttctc agcgcacgcg aagccgaaaa
     5341 gctcgtcggg acgccctaca gggatgccaa ccggatgatg caggcgttcg tgcaggatgg
     5401 cgtccttgag gtggtgaaga agggcgccgg catgaaggcc agccgctacc gcttcgtctg
     5461 ggatgacacc gcgagtggca attcctccag cgcccccatg gtggccgcgc ccgccgcacc
     5521 cagcagcgcc ggcaccgcgc cggcagcgtg gaagatccct gccgactggc aaaacgccgc
     5581 ctgacgccat ggaagccgcc accgactccc cgccacccat gccctggcgc ctcatgctgg
     5641 cggcgatggc tggcgatctg gcgccaccgg agcccatcca gcccggccca gcacaccgcc
     5701 accgcgaggg catgacctgt cttcccgccc cgcccttccc cttcgccgcg atctggtgcg
     5761 accgggagcc cgtggtgcgc gggcatctgg cgctgtgggc tgaccgatga acgcgcatcc
     5821 aaggcccgcg cacacgccgc acgcgggcgc actcgccacc atgggcgcca ccgggtcgag
     5881 ggcgggccgc gccgacgctg cacgggtgat gctggcacgc tcgctggcac tgcgcccgga
     5941 ccacgccccc acgctgctga actacgggat cgtcctgcac cagcttggcc gagacgcaga
     6001 ggcgttgctg acagtcgagc gcgccctatc gctcaacccc gagttcgccc gcgcctgggg
     6061 ctgctacgcc tcgttgctgc atcgcaaggt ccaacgcagc ggcgaccaag caagcctggg
     6121 cgctgcggcc tatgcctacc gcactgcatg ccgtctcggt gacggcgacc cggagagcat
     6181 cgccttcgca ctggcctcgc ttggcgccgc cgaacagccc ggcatcgcgc cacgggccta
     6241 cgtcgagcgc ctgtttgacg actacgcgcc caccttcgat tcgcacttgt gcgacggctt
     6301 gggctaccgc gcgcccgaat tgctggtgaa cgcgctgact cgaactcgcg gcacggcgcc
     6361 tgcagacatc gcggacctgg gttgcggaac aggcttgtgc ggcccactgt tgcgccctgt
     6421 cgctcggcgg ctggcgggtg tagacctgag ccgacgcatg ctggatatgg ccgcgcagcg
     6481 cgcctgctat gacgcgctgg cgcaatgcga catcgtggac tttctgtgca gtcatcccgc
     6541 gacgtttgac ctcgctgtgg cggcagacgt gctcgtctac atcggcgacc tgctcccgct
     6601 gttccaggcc gcgcacagcg cgctgcggca tggcggctgg ctggccatca cggtcgagcg
     6661 caatgatggt gaaggcttcg ggctccatgt ctcacggcgc tacacccaca gcatcaccta
     6721 tctggcagag acggcagcac gcgcaggatt cgaggtcgtg gcgctgacgg caacgaccgt
     6781 gcgtatggac gctggcctcc cggtgaaggg ggcgcttgtg ttgctgcgac gttcaccatg
     6841 aagaccgccg ccgccgccgt gagccgtcag cccaggaact ccccgagcgt cagttcggcc
     6901 tgtgattcga cccactcgat ggcactctcg cgtggctctg gctcagacag ccacggtgcg
     6961 cccgcccgta ccatctcgcc gatgctggaa gtagccagca cctgtgcgat gaggtcgtca
     7021 ggatcgtcca cagcgttgat ccggacaccc tgcatgtcca tcaaccaagt cactatcgcc
     7081 agcgcttcag ggtatggccc gaaggcttcg atcaggtcgt acaacttgcc cctgatggct
     7141 ggcatgtact cgtcctcggt ttcgatctgt accgccagtg tgtaggggcg cgaggctggc
     7201 acccaccatg acttcccttc caccatcacc agttccattc acgcaccccc tgtcgagtgg
     7261 tcaggactcc gcacgcacgg cgcccttttg cgctgtgtcg gtctgccagt cctggctgaa
     7321 catttcgttt tcagttggag tcgaacgacc atgagcaaag gtggcaagcg ctggggtgcg
     7381 gggcgtccgg gctggcatgt gaaggccgag cagtgcctgc gcctggatgt gcgccactgg
     7441 cagcgcgacg ggctgctggc gcccggccgc atgggcgagt gggagtggcg cgatgccact
     7501 gggcacaagg tggccggtgt cacgttcacc gtcaccgaag gcgctgtgac cttgacgcgg
     7561 ggcgggcgcc tgccgagtgg ggtgcgtggc aacgttgcca ctctgggcgc tgcggcatgt
     7621 ccaccggctg gcaatattgc cacccaggtg ctgccgatca cccgcaccgc gtgccacttc
     7681 ggcggcgacc gcccatggtt cgcctgcccc caatgccacc gccgggccgc cgtgctgtac
     7741 ctgcgggacc acttcggctt cgcctgccga ccctgcgcac aggtggcata tgccagccag
     7801 agtgaggatg ccattgcgcg ggcgtggcgc tgccagggca aggtggaagg ccggcttggc
     7861 gcgcatggat cgaagccacc gggcatgcac cgcaccaccc acgcgaagct ggcggcgaag
     7921 atcagcgcat gcgagcagcg gcgactggcg gcgctggtgg cttggcttcc atcccatcgc
     7981 aggccagcgt gacgcacgcc ggcagttggc gcccctgaat ctgcgctgct gggtcgaaga
     8041 aataccagcg ccggaggttc cacgcctgga agaattacca gcagcgccgt gaatacgccc
     8101 cgccaccccg gccgccctct tgcgtcactg cacgcagtcg aagacaaact cggctgatct
     8161 cgggctcggg cgctcgatca ttcgggcata ccggccatgc ttcgcgcact caacatcggc
     8221 cagcttctgc gactctgcaa tggcatggtc gggcgcccgc acgaccaccg tgcgagggct
     8281 gctgctgacc acctgggctg cgcacccgga cagtccaata cttaccgcga aaagcgccat
     8341 cgagtgcgcc aaccgaattc caactgccgc catcctgttc cccctcgttc gcaagcgtcc
     8401 aaccgttgcg cgggagcatc attgccccgc gttcttggtc gatccgtgtg gtccatcgtg
     8461 gccgggatga cggaccagat caaggggcgt ccgggcctta gcagcaaaga aaaatcgggg
     8521 aacagaccgg ggaactggcg tgggtcatcg gcaagaaaac ccagcattta tgcgggttgc
     8581 aggctacttt tcgattccct tcaccgctcc acgcaccaca cgtgccgatc agccccacac
     8641 ctcctgggcg gtctccac
//
LOCUS       DAOORV010000141         8593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10166_length_8593_cov_6.709417, whole genome shotgun sequence.
ACCESSION   DAOORV010000141 DAOORV010000000
VERSION     DAOORV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10166_length_8593_cov_6.709417"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKV56_14880"
     CDS             complement(<1..380)
                     /locus_tag="PKV56_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69142.1"
                     /translation="MPREPGRTNSRTSLRAAAVVGILVLLSVIYLGLQRLRPPENTGR
                     SIPVAEASNEQREASAVTHSAGASTAAVRAGLPASGAPAPRGPVSPVATVRLATKAPA
                     ARATDAAPPVTLPASSMAVPSAPA"
     gene            complement(827..2959)
                     /locus_tag="PKV56_14885"
     CDS             complement(827..2959)
                     /locus_tag="PKV56_14885"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOX69143.1"
                     /translation="MTETVHLRVAVDTPQHTALREPLTYLGDPALPPGTLVRVPLGRR
                     EVTGIVWAQRSGAVAETGAPTIELKPLLHALTSLSPLSERWCQLIEFAAAYYQRGIGE
                     VALSVLPPELRKLDDGQIRQRISRLHKGLAKLTPADAVVSQPETDGVAPALDLAQTRA
                     LAELMAMQVPTSEPGQSGAPTVLLHGITGSGKTEIYLRAAAQALSEGRQVLVLVPEIN
                     LTPQLEARFAERFAGRHIVSMHSALTPAQRLRGWLAAHLGSADLVLGTRLAVFASMPR
                     LGLIVVDEEHDPSYKQQEGARYSARDLAVYRGRLEGVTVLLGSATPSLESWQRAEEGR
                     YRRLSLPARIGGGALPRVRLVDMNQQPRAKGGAAISEPLLAALQQRIGRGEQSLVFLN
                     RRGYSPVLHCGDCGWKSGCPHCSAWRVFHKLDRTLRCHHCGFTERVPQACPDCGNLDI
                     APMGRGTERLEEQLAELLIRPDGSPVRIARIDADSTRGKGALEAQLGAVHAGDVDVLV
                     GTQMITKGHDFRRITLVAAVNPDTSLFSSDFRAPERLFALLMQAAGRAGRDAAHAAAS
                     EMWVQTWHPQHALYAALRSHDFEAFAASQLAERRMAGLPPFSHLALLRAEARTADAAR
                     SFLQDAAQAAEGAADSPAVTVYSPVPTPIARVANVERMQLLIESASRASLQRFLAEWL
                     PALHAMRRERTADQRILRWAVDVDPLAI"
     gene            complement(3339..4433)
                     /gene="hemE"
                     /locus_tag="PKV56_14890"
     CDS             complement(3339..4433)
                     /gene="hemE"
                     /locus_tag="PKV56_14890"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOX69144.1"
                     /translation="MYAPLQNDTFLRACLRLPTDHTPVWLMRQAGRYLPEYRATRAKA
                     GSFMGLATNTDYATEVTLQPLDRYALDAAILFSDILTVPDAMGLGLSFALGEGPKFER
                     AVRDEAAVDLLAVPDMDKLRYVFDAVTSIRKALKGRVPLIGFSGSPWTLACYMVEGAG
                     SDDYRLVKTMLYSRPDLMHRILAINADAVALYLNTQIESGAQAVMVFDSWGGVLADGA
                     FQEFSLAYTKRVLQQLKREHDGQRIPHIVFTKGGGLWLEEMADLGPDVLGLDWTVNLK
                     SARARVGDRVALQGNLDPNVLFAGPEQIRAEVTRTLESFGAPGAPRDGHVDGHIFNLG
                     HGISQHTPPENVTALVDAVHEVSRRLRQPG"
     gene            4513..5433
                     /gene="cysK"
                     /locus_tag="PKV56_14895"
     CDS             4513..5433
                     /gene="cysK"
                     /locus_tag="PKV56_14895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOX69145.1"
                     /translation="MRADNILQTIGQTPHIRINRLFGDGSRIFIKSERSNPGGSIKDR
                     IALAMVEAAEASGALVPGSTIIEPTSGNTGVGLAMVAAVKGYKLVLVMPESMSIERRR
                     LMLAYGASFELTPREKGMNGAIARATELVAATPNAWMPQQFENPANIDVHVRTTAVEI
                     ANDFPDGIDALITGVGTGGHLTGCAQVLKAKWPQLKVYAVEPAASPVISGGKPSPHPI
                     QGIGAGFIPKNLHTGLLDGTIQVEAEAAREMARRCAREEGMLVGISSGATLAAIAQKL
                     PELPAGATVLGFNYDTGERYLSIEGFLPVE"
     gene            5518..6126
                     /locus_tag="PKV56_14900"
     CDS             5518..6126
                     /locus_tag="PKV56_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOX69146.1"
                     /translation="MLRRLWLAALALTATLALSGCGYNQFQQLDEEVTAAWAEVLNQY
                     QRRADLIPNIVATVKGEANFEQETLTKVIEARSKATSIQATPELVNDPAAFQKFQAAQ
                     GELTGALSRLMVVAEQYPNLKANQGFQDLRVQLEGTENRVTVARNRYIKAVAEYNVLA
                     RSFPNNLTAMMFGYTVKPSFTVQNEAEISRPPAVNFDKPASK"
     gene            6271..7131
                     /locus_tag="PKV56_14905"
     CDS             6271..7131
                     /locus_tag="PKV56_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOX69147.1"
                     /translation="MTGAAASAQVVLPVPPLAARVIDQTSTLDEGQRASLVAKLAAIE
                     QQTGSQIVVLLVPSTLPEDIAAYSQRVADTWKIGRRDVGDGLLIVVAKGDRRVRIEVA
                     KTLEGAIPDLAAKRVIDGQITPAFRAGDYAGGLNAAVDQLASRIRGEALPAPKHSNAR
                     GNSGPFGVNLEALAIFFFVAVPMVGALLTSLLGRKLGSLATGGVAGAVGWWLSTSLLI
                     AGGAGLVALVLVGIFGVGSSRSTRGRDGPPIIWGGGSSGGGWGGGGGGGGFSSGGGGD
                     FGGGGASGNW"
     gene            7159..7665
                     /locus_tag="PKV56_14910"
     CDS             7159..7665
                     /locus_tag="PKV56_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOX69148.1"
                     /translation="MNRLIRILKHRWLDASDARRALGDDALHEIEARIAASERTHTGE
                     LRVCVEAGLPLSYLWRGATARDRAISMFGKLRVWDTESNSGVLIYLLLAEHRIEIVAD
                     RGLTHRVPASTWQAISGEMAAAFRERDYARGVLDAVDRVGALLTQHFPADLQAVNPNE
                     LADRPDVR"
     gene            complement(7747..8163)
                     /locus_tag="PKV56_14915"
     CDS             complement(7747..8163)
                     /locus_tag="PKV56_14915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOX69149.1"
                     /translation="MATLHVDVVSAEESIFSGEAKFVALPGEMGELGIYPRHTPLITR
                     IKPGAVRIVRADNDAEEFVFVAGGILEVQPGTVTVLADTAIRGHDLDEAKANEAKQRA
                     EEAMKNAKSDIDFAKAQGEFAAMAAQIAALRKFRKK"
     gene            complement(8307..>8593)
                     /locus_tag="PKV56_14920"
     CDS             complement(8307..>8593)
                     /locus_tag="PKV56_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOX69150.1"
                     /translation="YKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFSVAEVF
                     TGAPGKYVTLKETIRGFKMIVSGECDSLPEQAFYMVGTIDEAFEKAKKMQ"
BASE COUNT         1509 a         2828 c         2825 g         1431 t
ORIGIN      
        1 aacgcaggag cgctgggcac ggccatcgac gatgccggca acgtgaccgg cggcgctgca
       61 tcggtagccc gcgccgccgg cgccttggtc gccaggcgca ccgtggcgac tggcgagacc
      121 ggccccctcg gtgcgggcgc accagaagcc ggcaggccgg cgcggaccgc cgctgtcgaa
      181 gccccggcgg aatgcgtcac ggccgaagct tcgcgctgct cgttcgatgc ttcagccacc
      241 gggatggacc ttcccgtgtt ctcgggcggc cgcagacgct gcaggcccag gtagatgacc
      301 gacagcagca ccaggatgcc gaccacggcc gctgcgcgca gcgaggtgcg agagttcgtg
      361 cggccaggct cccgcggcat ggaatccgtg ggcggatgcg gcatcttcga agccgcgcgg
      421 gtcgccctgg aacttcggcg cggccgatcg gcggcgatcg ccggctcggc ttctgccctg
      481 gttgacccgc tccgctgagt aggcgcccca gccggtgcgg ggatctcccg agcagcggat
      541 acagaaggcg gttcggtcac ccgcagctcg gccggcagcg tcaggctcgt cggaacagcc
      601 gcttcgccgg ctggcgaagc ggcccgtgcc gacaccgccg aagcacagtt caggcaggga
      661 tcagcaagac gggcgaggcg atgaccgcag atcggacact tcatcggggt gtgcaggcgc
      721 aagcacgggc tcacgctcct tcgcccattt cggcatcagg gctgcagaac ttgagccccg
      781 agccctacag gatggtcgaa cggtctcgcc gaccggcgcg ggatcctcag atcgccagcg
      841 gatcgacgtc gaccgcccag cgcaggatgc gctggtctgc ggtccgctcg cgccgcatcg
      901 cgtgcagcgc gggcaaccac tcggccagga agcgttgcag cgatgcgcgc gacgccgact
      961 cgatcagcaa ttgcatgcgc tcgacgttgg cgacccgcgc gatcggggtc ggcaccggcg
     1021 agtacaccgt cacggcgggg ctgtcagctg caccttcggc agcctgcgct gcgtcctgca
     1081 ggaagctgcg cgccgcatcg gccgtgcgcg cctcggcccg cagcagcgcc aggtgggaaa
     1141 acggcggcaa tccggccatg cggcgctccg ccagctgcga cgccgcaaac gcctcgaaat
     1201 cgtggctgcg cagagcggca tagagcgcat gctgcggatg ccaggtctgt acccacatct
     1261 cgctggcagc ggcgtgggcg gcatcgcgcc cggcgcggcc agccgcctgc atcagcagtg
     1321 cgaacaacct ctcgggcgcg cggaagtcgc tgctgaacag cgaggtatcg gggttgaccg
     1381 ccgcgaccag cgtgatacgc cggaaatcat ggcccttggt gatcatctgc gtgcccacga
     1441 gcacatcgac gtcgccggca tgcaccgcgc ccagctgcgc ttccagcgcg cccttgccgc
     1501 gcgtgctgtc ggcgtcgatg cgggcaatac gcaccggact gccatcgggc cggatcagca
     1561 gctccgcaag ctgttcctcg aggcgctcgg tgccacggcc catcggcgcg atgtcgaggt
     1621 tgccgcagtc agggcaggcc tgcggtacac gctcggtgaa tccgcagtgg tggcagcgca
     1681 gcgtgcgatc gagcttgtga aagacgcgcc aggcgctgca atgcgggcag ccgcttttcc
     1741 atccgcagtc gccgcagtgc agcaccggcg aatagccccg gcgattcagg aacacgaggc
     1801 tctgctcgcc gcgcccgatg cgctgctgca gcgcggccag cagcggctcc gagatggcgg
     1861 cgcccccctt cgcgcgcggc tgctggttca tgtcgaccag gcgcaccctt ggcagtgccc
     1921 cgcctccgat gcgtgccggc agggacaggc gccgataacg accctcctcg gcgcgctgcc
     1981 agctctcgag cgagggcgtc gcggagccca gcagcaccgt gacgccttcg agcctgccgc
     2041 gatagacggc cagatcgcgc gccgagtagc gggccccttc ctgctgcttg tacgagggat
     2101 cgtgctcctc gtcgacgacg atcagtccga gccggggcat cgatgcgaac accgcgagcc
     2161 gcgttccgag cacgagatcg gctgatccga ggtgcgccgc gagccagccg cgaagccgct
     2221 gcgccggcgt cagcgcgctg tgcatcgaca cgatgtgccg cccggcgaag cgttcggcga
     2281 atcgggcttc gagctgcggc gtcagattga tctcgggcac cagcaccaga acctggcgtc
     2341 cttcggacag cgcctgggcg gcagcgcgca ggtagatctc ggtcttgccg ctgccagtga
     2401 tgccgtgcag gagcaccgtc ggggcgccgc tctgacctgg ctcgctggtc ggcacctgca
     2461 tggccatgag ttcggccagc gcccgggtct gcgcgaggtc gagcgccgga gcaacgccat
     2521 ccgtttcggg ctgactcacc accgcgtcag ccggtgtcag cttcgccaga cccttgtgga
     2581 ggcgagagat gcgctgccgg atctggccat cgtcgagctt gcgcagttcc ggcggcagca
     2641 ccgacaaggc aacttcgccg atgccccgct ggtagtaggc ggcagcgaac tcgatcagct
     2701 ggcaccacct ctcgctcagc ggcgacagcg aagtcagcgc gtgcagcagc ggcttgagtt
     2761 cgatcgtcgg ggcacccgtt tccgcgacag cgccagatcg ttgcgcccac acgatgccgg
     2821 tcacctcgcg gcgtccgagg ggaacacgca ccagcgtgcc gggcggcagc gcggggtccc
     2881 ccaggtaggt caaaggctct cgcagcgcgg tgtgctgagg cgtatccacc gcgaccctca
     2941 ggtggacggt ttcagtcatt gctgtcaacc cccgacttaa acgccaaagc tagagtcttg
     3001 ctgagacaaa ccccataagc ccctgatttc aatgcacttt ccgggctatt caccaaatat
     3061 gtggataact ttgtgggcaa cctgccaaaa ccgcggctaa agcgttgatt catcgggctc
     3121 agccttacgt tgcccacttt cgcggcaaca cgcaagactc aatgaaatca atgacttagc
     3181 gatctttgaa taaatttttg tgttgcggcg cagcaggatc cgtcagccaa acaggcatca
     3241 acagaaatgg ggataagtca atgcctgagc acttactcac ctgctgatcg agcagacacg
     3301 agaccgcctg cggcctgcat cggccaagcg aaggtacgtc agcccggctg acgcaagcgg
     3361 cgcgacacct catgaaccgc atcgaccagc gccgtgacgt tttccggcgg cgtgtgctgg
     3421 ctgatgccgt gaccgaggtt gaagatgtgg ccgtcgacgt ggccatctcg cggggcgccc
     3481 ggcgcaccga aactttccag tgtcctggtg acctcggcgc ggatctgctc cggtccggcg
     3541 aacagcacat tcggatcgag gttgccctgc aacgcgaccc gatcgccaac gcgtgcacgc
     3601 gccgatttca ggttgacggt ccagtcgagc ccgagcacgt caggccccag gtcggccatt
     3661 tcttcgagcc acagcccgcc gcccttcgtg aacacgatgt gggggatgcg ctgaccgtcg
     3721 tgctcccttt tcaactgctg cagcacgcgc ttcgtgtagg ccaggctgaa ctcctggaac
     3781 gcgccgtcgg ccaatacgcc gccccagctg tcgaagacca tcactgcctg agcaccagac
     3841 tcgatctggg tgttgaggta cagcgccacc gcatcggcgt tgatcgcgag gatccgatgc
     3901 atcaggtcgg ggcggctgta cagcatcgtc ttgaccaggc ggtagtcgtc agaccccgca
     3961 ccctcgacca tgtagcacgc gagcgtccag gggctgcccg agaaaccgat cagcggcacg
     4021 cgccccttca gcgccttgcg gatcgaggtg acggcgtcga acacatagcg cagcttgtcc
     4081 atgtcgggca cggccagcag atcgacggcc gcttcgtcac gcacggcacg ctcgaacttg
     4141 gggccctcgc cgagcgcgaa gctcaggccc agccccatcg cgtcgggcac ggtcaggatg
     4201 tcgctgaaca gtatcgcggc gtcgagtgcg tagcgatcca ggggttgcag cgtgacctcg
     4261 gtcgcgtaat ccgtgttggt cgccaaaccc atgaaactgc cagccttcgc ccgcgtcgcg
     4321 cggtactccg gcaggtagcg tcccgcctgc ctcatcagcc agacgggcgt gtgatcggtc
     4381 ggcaggcgca agcaggcgcg caggaaggtg tcgttttgca gaggtgcata catggcggaa
     4441 ttatcacgcc cataatttcg cccatccacc gattccgccc cgggaaaaca cagggcacca
     4501 caggagccga ccttgcgcgc tgacaacatc ctccagacca tcggccaaac gccgcacatc
     4561 cgcatcaatc ggttgttcgg cgacggttct cgtatcttca tcaagagcga acgctccaat
     4621 ccaggcgggt cgatcaagga ccgcatcgcg ctggcgatgg tcgaggcggc cgaagcctcc
     4681 ggtgcgctcg tgccgggctc gacgatcatc gaaccgacct cgggcaacac cggcgtcggg
     4741 ctcgccatgg tcgcagcggt caagggctac aagctggtcc tggtgatgcc ggaaagcatg
     4801 agcatcgaac ggcggcgcct catgctggct tacggtgcct cgttcgagct gacacctcgc
     4861 gagaaaggca tgaatggcgc catcgcccgc gcgaccgagc tggtcgccgc gacgccgaat
     4921 gcctggatgc ctcagcagtt cgagaatccc gccaacatcg atgtgcatgt gcgaaccacg
     4981 gcagtcgaga tcgccaacga ctttcccgat ggcatcgatg cgctgatcac tggcgtgggg
     5041 accggcgggc acctcactgg atgcgcgcag gtcctgaagg cgaaatggcc ccagctcaag
     5101 gtgtatgccg tcgagccagc ggccagcccg gtgatcagtg ggggcaagcc ctcgccacac
     5161 ccgatccagg gcatcggcgc aggcttcatc ccgaagaacc tgcacaccgg cctgctggat
     5221 ggcacgatcc aggtggaggc cgaagcggca agagagatgg cgcgtcgctg cgcccgcgag
     5281 gaaggcatgc tggtcggtat ctcgtcaggc gcaacgctgg cagccatcgc ccagaagctg
     5341 cccgagctgc cggccggcgc caccgtgctg ggcttcaact acgacaccgg cgagcgatat
     5401 ctctcgatcg agggattcct tccggtggag taacccctgc cggggcagcc cgagtgcgcg
     5461 gctcgcgcaa tgccgcggca gtagcatggg ggaattccat tcaacgggag ctcccccatg
     5521 ttgcgcagac tttggctggc cgctctggcc ttgaccgcga cgctcgcgct gagcggttgt
     5581 ggctacaacc agttccagca actcgacgaa gaagtgacgg ccgcctgggc cgaagtgctg
     5641 aatcagtacc agcggcgcgc agacctgatt cccaacatcg tcgccaccgt caagggcgaa
     5701 gccaacttcg agcaggagac cctgaccaag gtgatcgagg cgcgctcgaa ggcgaccagc
     5761 atccaggcca cgccggagct ggtcaacgat ccggctgcct tccagaaatt tcaggcagcg
     5821 cagggcgagc tgacaggagc gctgagccgc ctgatggtgg tggcggagca gtatccgaac
     5881 ctgaaggcca atcagggttt ccaggatctg cgggtgcaac tcgagggcac cgagaaccgc
     5941 gtcaccgtgg cgcgcaatcg ctacatcaag gcggtcgccg agtacaacgt gctggcccgg
     6001 agcttcccca acaacctgac ggcgatgatg ttcggctaca cggtcaagcc cagcttcacg
     6061 gtgcagaacg aagccgaaat ttccaggccg cccgcagtga atttcgacaa gcccgcgtcg
     6121 aagtgacgtc aacccatgcc acggtggcgc gtggcgacat cctttgatgc gttcatgagc
     6181 gcccggtctg cctggcgccg ggaacctccg gttcgaccag gggctgcggt ccgactggcg
     6241 acgctgtttc tggtcgcgat cctgcagttg atgacaggcg ctgcggcaag cgcccaggtt
     6301 gtgctgccgg taccgcccct cgcggcgcga gtgatcgatc agacatccac gctcgacgag
     6361 ggccagcggg cctcgctggt cgcaaagctc gccgccatcg agcagcaaac gggcagccag
     6421 atcgtcgtgc tgctggttcc cagcaccttg cccgaggaca tcgccgcata cagccagcgt
     6481 gtggcagaca cctggaagat cggccgccgt gacgtcggag acggcctcct gatcgtcgtg
     6541 gccaaaggcg atcggcgcgt tcgcatcgag gtggccaaga ccctcgaggg tgcgattccc
     6601 gacctggcag ccaaacgcgt gatcgacggg cagatcacac cagcctttcg agccggcgat
     6661 tacgccggag gcctgaacgc ggcggtcgac cagctcgcct cacgcattcg gggtgaggca
     6721 ttgcccgcac cgaagcattc caatgccagg ggcaactcag ggccatttgg ggtcaatctg
     6781 gaagcgctcg cgatcttctt cttcgtcgcg gtgcctatgg ttggcgcttt gctgaccagc
     6841 ctgcttggac gcaagctggg ttctttggcc acgggcggcg tagcgggtgc cgtcggctgg
     6901 tggctcagca cgagcctgct gattgcaggc ggcgccggac tggtcgcgct ggtgctggtc
     6961 ggcatcttcg gcgtgggttc gtcccggagc actcgcggcc gggacgggcc accgatcatc
     7021 tggggcggtg gcagcagcgg tgggggatgg ggtggcggag gcgggggcgg cggcttcagt
     7081 tcgggcggtg gtggcgattt cggcggaggc ggcgcctccg gcaactggta gccggcgccg
     7141 ccactgaaga gcgacgacat gaacagactg attcgcatcc tcaagcatcg atggctcgac
     7201 gcgtccgacg cacgccgtgc gcttggcgac gatgcattgc acgaaatcga ggcgcggatc
     7261 gctgccagcg agcggactca cacgggtgaa ttgcgggtct gcgtcgaggc cggtctgccg
     7321 ctgagctacc tttggcgggg tgcgacagcg cgtgatcgtg cgatctcgat gttcggcaag
     7381 ctgcgagtct gggacaccga gagcaacagc ggcgtgctga tctaccttct tctggccgag
     7441 caccgcatcg agatcgttgc cgaccgcgga ttgacgcatc gcgtgccggc gtcaacctgg
     7501 caggcaattt ccggcgagat ggcagccgcc ttccgtgaga gagactatgc gcgcggtgtg
     7561 ctcgacgcag tcgaccgcgt gggtgcgctg ctgacgcagc atttcccggc cgacctccag
     7621 gcagtcaacc cgaacgaact ggcagatcgt cccgacgtgc gctgacgcgt accgcgcacc
     7681 cgagtcgcac ggcgaaaaaa aacccgccac gacgggcggg ttgctttcaa gtcgtcgtcg
     7741 ctgtctttac tttttccgga attttctgag tgccgcgatc tgcgccgcca tggcagcgaa
     7801 ctcgccctgt gctttcgcga aatcgatgtc gctcttggcg ttcttcatcg cctcttcggc
     7861 tcgttgcttc gcttcgttgg ccttggcctc gtcaagatcg tgaccgcgga tggcggtgtc
     7921 ggcaagcacg gtgaccgtgc ccggctgaac ttcaaggatg ccgccggcca cgaatacgaa
     7981 ctcttccgca tcattgtccg cgcgaacaat gcgcaccgca cctggcttga tgcgcgtgat
     8041 caagggcgtg tgacggggat agatgcccag ctcgcccatt tcaccaggca gggcaacaaa
     8101 cttggcctcg ccggagaaga tggactcttc ggcggatacg acgtctacgt ggagtgtggc
     8161 catgttgatg ttccgttagc ttgaggtttc gagcacaaga aaaggttcag ggagcaatcg
     8221 gttgccgact tggccaggcg tgccttgcaa gcgcggactc gcgtccgccg ggcaggtcaa
     8281 cgccgccaac tcgccaaacg caccgcttac tgcatcttct tcgctttctc gaatgcctcg
     8341 tcgatcgtgc cgaccatgta gaacgcctgc tccggcaggc tatcgcactc gccggaaacg
     8401 atcatcttga agccgcgaat ggtttccttc agagtcacgt acttgcctgg tgcgccagtg
     8461 aacacctcgg caacgctgaa cggctggctc aggaaacgct ggatcttccg ggcacgggcc
     8521 acggccagct tgtcttccgg cgacagttcg tccatgccca ggatggcgat gatgtcgcgc
     8581 agttccttgt agc
//
LOCUS       DAOORV010000142         8584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10180_length_8584_cov_8.707586, whole genome shotgun sequence.
ACCESSION   DAOORV010000142 DAOORV010000000
VERSION     DAOORV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_8584_cov_8.707586"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="PKV56_14925"
     CDS             <1..119
                     /locus_tag="PKV56_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69151.1"
                     /translation="YRAGKDKAFNALVGQAMKATKGKANPAQVTELLKKKLG"
     gene            complement(88..855)
                     /locus_tag="PKV56_14930"
     CDS             complement(88..855)
                     /locus_tag="PKV56_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOX69152.1"
                     /translation="MASELLTRQDARGDRLCNALCGGVLLLMCCSVQAAGPIYSCIDA
                     NGKRLTSDRPIPECTSRDQRVLNADGSTRKVVPPKMTADEQAEFDARERAKAAVRAAE
                     MDAIRRDRNLRQRYPTEAAHQKARQSALNDLDRTLQLSQKRLEVLAAERKPLLDEAEF
                     YVGRQMPTRLKQQLDGIDAATEAQRALMQNQQAETLRINATFDAELARLRALWAGAQP
                     GSLGPLLPASTPKPASASTPPSTPANGLSPASSSATR"
     gene            complement(845..1546)
                     /gene="pyrE"
                     /locus_tag="PKV56_14935"
     CDS             complement(845..1546)
                     /gene="pyrE"
                     /locus_tag="PKV56_14935"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOX69153.1"
                     /translation="MSPSGRVDAETDDLAQDFVRFSLEAGVLRFGEFKTKAGRLSPYF
                     FNAGLFDDGAKLGRLAEFYARRLQQARASGALQFDMLFGPAYKGIVLAAAVAVELARL
                     GVNLPYAYNRKEAKDHGEGGTLVGAPVRGRVLIIDDVISAGTSVRESVAMIEAAGGTP
                     SAVVIALDRQEKATEGGQDAPWSAVQFVQQQLGLQVVAIATLADLLHVLQTTDAPALG
                     VHAERVASYRTRYGV"
     gene            1559..2365
                     /locus_tag="PKV56_14940"
     CDS             1559..2365
                     /locus_tag="PKV56_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOX69154.1"
                     /translation="MLRLVTLNLNGIRSAATKGFEAWAAASAPDCMGVQEVKAQADTV
                     DGRFDRVAGMTGHFHYAQKKGYSGVGLYTRHAPSLIRSGIGNPEFDDEGRWIEACFDD
                     DNRPEFPRFSVISCYFPSGSSGEERQQAKFRFLALMAPHLAQLRAEREFVLLGDINIA
                     HKEIDLKNWKGNLKNSGFLPEERAWMTHLLDAESGVGLVDVFRTLDPHPDRYTWWSNR
                     GQAWAKNVGWRIDYQLATPGIAARALRADIHLEPRFSDHAPLTIDYDFVL"
     gene            2636..3073
                     /locus_tag="PKV56_14945"
     CDS             2636..3073
                     /locus_tag="PKV56_14945"
                     /inference="COORDINATES: protein motif:HMM:NF022224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HOX69155.1"
                     /translation="MNVKRLDGPLLNFWVAKSAGLQLVGEDPGPDSTHDTNSGFWHPH
                     TYDPSHDWSHAGSVIAEEWYRIEDTLLEWFGPEWSQVPAISKDPLKWFLRAYVATQFG
                     DEVEDVSSAGSCKLRNTDSTPVPATPSVKPRSWMPHWLRQLND"
     gene            3244..3906
                     /locus_tag="PKV56_14950"
     CDS             3244..3906
                     /locus_tag="PKV56_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOX69156.1"
                     /translation="MALDALEFETAAQPTASIIVLHGLGADGNDFVPIAEELELDTVG
                     PVRFVFPHAPVRPVTINGGMAMRAWYDLLQADLVRREDEAGLRASRLDIEALIARERA
                     RGVPASRIVLMGFSQGCAMTLLTGLRHAERLAGLVGLSGYLPLADTTASERAAASVAL
                     PIFLAHGRSDTVIPLARATASRDVLQSLGCVVEWHEYPMAHSVCPQEVADLNRWLLGV
                     LA"
     gene            complement(3920..4894)
                     /locus_tag="PKV56_14955"
     CDS             complement(3920..4894)
                     /locus_tag="PKV56_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX69157.1"
                     /translation="MSTNTDFDPQAQFEPDAAVAAWHRLGCSCATHSRRVFGRAVLGA
                     GAALAMPAWAREGVQVDSQSTLAKLVPAEEVETAAAQQYQQMLKQAAEKNALAPADHP
                     QVLRLRAIARRLIPFSNAPNLRSTSRAASWKWEVNLIGSKQINAFCMPGGKIAFYYGI
                     LETLQLSDDEVAQVMGHEIAHALREHARERMGKSAATRGAIELGATLLGLGGGGRYLA
                     DMGGQLLTMKFGRDDESEADKIGLDLAARGGYDPRAGVRLWQKMGAANKGAPPQWLST
                     HPAGTTRIKDLENNIPAVEGLYVRADKPARRFEPPKPSPSVTDAGRSS"
     gene            complement(4964..6586)
                     /locus_tag="PKV56_14960"
     CDS             complement(4964..6586)
                     /locus_tag="PKV56_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69158.1"
                     /translation="MPDHEPVERARPLASSRARFDSGAWHRMAVVTFLGFASGLPLAL
                     TGQAMQAWLSLEGLDLATIGFLSLVGLPYTFKFLWAPLMDRFELPWLGRRRGWLVLTQ
                     LALAGALWWLAATPPAEATRAFALLAVLVAFVAASQDVVIDAYRTDLLAARERGLGSS
                     LNVLGYRLAMILSGGVVLIWVDPSNAGSPGGWTWPEVYRFMAALMGAAAVLSALVLPR
                     VHAAGDRSSVARQDLLGFAALVAAVAVGFVVSDRLGPPLARALLAPWFDGSALDAGLK
                     GKWTDLLALLLGLAFTLPLAAWAARAAKFQTLLGGLNSYFSQRGAAAFLIFIVLYKLG
                     DAFAGSLMTPFLLKSMAFGPAEVGVVNKLIGLWLTIGGALLGGLLMLKLGLWRALLLF
                     GVLQMLSNLGFWWLAVNGKGALPGFVLPPFDWGFVKLVQSTEIDGGLLMVIAAENLSG
                     GMGTAAFVAFLMSLCNQRFTATQYALLSAFASVGRVWVGPLAGVLSEAIGWPTFFIVS
                     TLVALPALCMLWWLRDSVRELEADPDAPTSGD"
     gene            6727..7236
                     /locus_tag="PKV56_14965"
     CDS             6727..7236
                     /locus_tag="PKV56_14965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOX69159.1"
                     /translation="MSAIPQALPPSFSSTDFRAALGTFATGVTVITAREASGKPVGLT
                     ANSFNSVSLTPPLVLWSLSRQASTMPAFARGSHYAINILAADQREIAERFARKDIDRF
                     DGIALRTGLSGAPVLDGVVAVFECFNRSQYEEGDHVIFVGEVEHCARREGAQPLIFHG
                     GRYYTELPI"
     gene            complement(7249..>8584)
                     /locus_tag="PKV56_14970"
     CDS             complement(7249..>8584)
                     /locus_tag="PKV56_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX69160.1"
                     /translation="ERAQSLDRSTYRITLGLRALDRYTYRITLGKPSPRFINNFTGTG
                     ALAREVVEAYGDKIMEHPVGTGPYVLAEWQRSSRMVLTRNPNFREQFYEAEPPADDPA
                     AQALHARMKGKRLPMIDRVEIAVVQESQPRWLAFLNAEHDMIERLPAEFSNLAIPNNR
                     VAPNLAARGISMVRTPGSDIAFTYFNMQDPVVGGYTPEKVALRRAIALAYDTQTEIRL
                     VRRNQAVPAESTLPPMTFGFDPKWKSEMSLYDPARAKALLDLYGYVDKNGDGWRDRPD
                     GGPLTLIYSTSPDQTSRQLNELWKKYMDAVHIKVEFKIGQWPEQLKAARAGKVMMWGL
                     GQSASSPDADGFLALAYGPQKGEGNLAFFDLPAFNKLYDQQTLLGDTPERLALMQEAS
                     KLLVAYMPMKVHAHRIATDLMFPWVIGYQRHPFMREFWRYIDIDPERAAARK"
BASE COUNT         1459 a         2905 c         2843 g         1377 t
ORIGIN      
        1 aataccgcgc cggcaaggac aaggccttca acgcgctggt cggtcaggcc atgaaggcca
       61 cgaagggcaa ggccaacccg gcccaggtca ccgagttgct gaagaagaag ctgggctgag
      121 accgttggcg ggcgtcgatg gcggtgtcga cgccgacgcc ggtttcggcg tggacgcggg
      181 cagcagcggc ccgagcgacc cgggctgtgc gcctgcccac aaggcgcgaa gccgcgccag
      241 ctcggcgtcg aaggtcgcgt tgatgcgcag cgtttctgcc tgctggttct gcatcagggc
      301 gcgctgggcc tcagtggcag cgtcgatgcc atcgagctgc tgcttgagtc gcgtcggcat
      361 ctgccgcccg acgtagaact cggcctcgtc gagcagcggc ttgcgctcgg ctgccagcac
      421 ctccagacgc ttctgcgaca gctgcagcgt tcggtcgagg tcgttgagcg ccgactggcg
      481 tgccttctgg tgtgcagcct cggtcgggta acgctgacgc aggttgcggt cgcggcggat
      541 ggcgtccatt tcggcagcgc gcacggccgc cttggcacgc tcgcgcgcgt cgaactcggc
      601 ttgttcgtcg gcggtcatct tgggcggcac caccttgcgg gtcgacccat ccgcgttgag
      661 cacccgctgg tcgcgggacg tgcattcggg aatcggccgg tccgatgtca gtcgcttgcc
      721 gttggcgtcg atgcacgaat agatcggacc cgccgcctgg accgagcagc acatcagcag
      781 cagaaccccg ccgcacagcg cattgcacaa ccggtcaccg cgtgcatcct ggcgggtgag
      841 gagctcagac gccatagcgt gtccggtaac tggctacccg ctcggcatgg acacccagcg
      901 ccggagcgtc ggtcgtctgc agaacgtgaa ggagatcggc cagcgtcgcg atcgccacca
      961 cttgcagtcc gagttgttgc tgcacgaact gcaccgccga ccacggtgca tcctgtccac
     1021 cctcggtggc cttttcctgg cggtcaaggg cgatgacgac ggcgctgggc gtgccaccag
     1081 ccgcctcgat catcgcgacc gattcgcgca ccgaggtgcc ggccgaaatg acatcgtcga
     1141 tgatcagcac ccggcctcgc accggcgcac cgaccagcgt cccgccttcg ccgtggtcct
     1201 tggcttcctt gcggttgtag gcatacggca ggttgacgcc gagccgcgcc agctcgaccg
     1261 ccactgcggc ggccagcacg atgcccttgt aggccggacc gaacagcatg tcgaactgca
     1321 atgcgcccga ggcacgggcc tgctgcaggc gccgtgcata gaattcggcg aggcggccga
     1381 gcttggcgcc atcgtcgaac aggccggcgt tgaagaaata gggcgacagg cgcccggcct
     1441 tggtcttgaa ctccccgaat cgcaacacgc cggcttcgag cgaaaacctc acgaagtcct
     1501 gggcaagatc gtccgtttcg gcgtcaacgc ggccacttgg gctcatctga ggaatcctgt
     1561 gctccgtctg gtcacattga atctgaacgg catccgatct gcagccacga agggcttcga
     1621 ggcctgggcc gccgcttcgg cgcccgattg tatgggcgtg caggaggtca aggcccaggc
     1681 cgacacggtc gatgggcgct tcgatcgggt ggcgggcatg accggtcatt tccattacgc
     1741 acagaaaaaa ggttactcgg gcgtcgggct gtacacgcgc cacgccccga gcctgattcg
     1801 cagcggtatc ggcaaccccg aattcgatga cgaggggcgc tggatcgagg cctgcttcga
     1861 cgacgacaac cgtccggagt ttccgcgctt cagtgtcatc agctgctatt tcccgagcgg
     1921 cagcagcgga gaggagcggc agcaggccaa gttccgtttt ctcgcactga tggcgccaca
     1981 cctggcgcag ttgcgcgccg aacgcgagtt cgtactgctg ggcgacatca acatcgccca
     2041 caaggaaatc gacctgaaga actggaaggg caacctgaag aacagcggct tcctgcccga
     2101 agagcgcgcc tggatgacgc acctgctcga cgccgagtca ggtgtcggtc tggtcgatgt
     2161 ctttcgcacg ctcgaccccc accccgatcg ctacacctgg tggagcaacc gcggacaggc
     2221 ctgggccaag aacgtcggct ggcgcatcga ctaccagctg gccacccccg gcatcgcggc
     2281 ccgggcgctt cgcgcggata tccacctcga gccgcgcttc agcgatcacg caccgctgac
     2341 catcgattac gacttcgtgc tctgaacgat cgctcggggt ccgaatcgcc cgaagccggg
     2401 ccggcaccga ccgggaaatt gggggagatc acccaggcaa cggttgaatt gacccagtca
     2461 gcgcgtccgg ggcaaatcgg gatcacgagc ctgcatgaaa attatcgttt ccattcaatg
     2521 acttggcgat aggcgtaccg aatgcttcct gcggcatgtt ccatgctgca gacgccgaaa
     2581 tccgctcggc gaggcacgtc gttgcgggat cggcgcgatg tgcacgggag ccgccatgaa
     2641 cgtgaaacga ctcgatggac cgttgctgaa tttctgggtc gccaaatccg ccggcctgca
     2701 gctggtcggc gaggatccgg ggccggactc cacgcacgac accaacagcg gcttctggca
     2761 cccgcacacc tacgatccga gccacgactg gtcgcacgcg ggatccgtca tcgccgagga
     2821 gtggtaccgc atcgaagaca cgctgctcga atggttcggg ccggagtggt cccaggtgcc
     2881 cgccatctcg aaggacccgc tgaagtggtt cctgcgcgcc tacgtggcca cgcagttcgg
     2941 cgacgaagtg gaagacgtct catcggcggg ctcgtgcaag ctgcgcaata ccgactcaac
     3001 acctgtccct gccaccccga gcgtcaagcc ccggtcctgg atgccgcatt ggctgcggca
     3061 gctcaacgac tgagacaaag gctgcgcgac gcgctcggcc ggtgacctgc cgtccaggca
     3121 gcgctggagg tcgacaaggt aaattcagcc caccgcccgg cgctcgcgcc gctggcgacg
     3181 gctcgggcgc ttcggccatg ccgggcgagc gcacggcaac cattcactac agagactttc
     3241 accatggcgc ttgacgcact cgaattcgaa accgctgcgc aacccacggc cagcatcatc
     3301 gtgctgcacg gcctgggggc cgacggcaac gacttcgtgc cgattgccga ggaactggag
     3361 ctcgacacgg tcggaccggt gcgtttcgtc tttccccatg caccggtgag gccggtcacg
     3421 atcaacggcg gcatggcgat gcgcgcctgg tacgacctgc tgcaggcgga cctggtgcgg
     3481 cgcgaggacg aagccggcct gcgagcctcg cggctcgaca tcgaggcgtt gatcgcccgc
     3541 gaaagggcgc gcggcgtgcc cgcatcgcgc atcgtgctga tgggtttctc gcagggctgc
     3601 gcgatgacgc tgctgacggg cttgcgtcac gccgagcgcc tggccggtct cgtcggcctg
     3661 tcgggttacc ttccgctcgc cgacacgaca gcctcggagc gcgcagcagc cagcgtcgcg
     3721 ctgccgatct ttctggccca cgggcgcagc gataccgtga tcccgctggc gcgcgcgacc
     3781 gcatcgcgcg atgtgctgca gtcgctgggc tgcgttgtcg aatggcacga gtacccaatg
     3841 gcgcattcgg tgtgcccgca ggaggtcgcc gacctcaacc gctggctgct gggcgtgctg
     3901 gcctgacggc gcgcgggcgc tagctgctgc gccccgcatc ggtcaccgaa gggctgggct
     3961 tcggcggctc gaagcgccgc gcgggcttgt cggcacggac atacagccct tcgaccgccg
     4021 gaatgttgtt ttccaggtcc ttgatgcgcg tggttcctgc cgggtgggtg gacagccact
     4081 gcggaggtgc gcccttgttg gccgcgccca tcttctgcca cagacggacg cccgcgcgcg
     4141 ggtcgtagcc tccccgggca gccaggtcga gaccaatctt gtcggcttcg gattcgtcgt
     4201 cacggccgaa cttcatcgtc agcaactgcc cgcccatgtc ggccagatag cgccccccac
     4261 caccgagccc gagcaatgtg gcaccgagct cgatcgctcc gcgcgtcgca gcagacttcc
     4321 ccatgcgctc ccgcgcgtgc tcgcgcagcg cgtgggcgat ctcgtggccc atcacctgcg
     4381 ccacttcgtc gtcgctgagc tgcagcgtct cgaggatgcc gtaatagaag gcgatcttgc
     4441 cgccgggcat gcagaaggcg ttgatctgct tgctgccgat caggttgact tcccacttcc
     4501 agctcgccgc acgcgaggtg ctgcgcaggt tcggcgcatt gctgaacggg atcagccgcc
     4561 gggcaatcgc acgcagccgc agcacctgcg ggtggtccgc cggcgccagc gcattcttct
     4621 ccgccgcctg cttcagcatc tgctggtatt gctgcgccgc ggcggtttcg acttcttcgg
     4681 ccgggaccag cttggcgagg gtcgactggg agtccacctg cacaccctcg cgcgcccagg
     4741 ctggcatcgc cagcgccgca ccggcgccga gaaccgcacg cccgaacact cggcgcgagt
     4801 gcgtcgcgca actgcaccca aggcgatgcc aggcggcaac cgccgcatcg ggttcgaact
     4861 gggcctgtgg gtcgaaatcg gtgttcgtgc tcatgtcgtc gggtcgatgg tggaaggctc
     4921 gacagcctcg atgatccgtc aagtgtcgct gcggggcagt tggtcaatcg ccggaggttg
     4981 gggcgtccgg atcggcttcg agttccctca ccgaatcgcg cagccaccag agcatgcaca
     5041 acgccggcag cgccaccagc gtggacacga tgaaaaaggt cggccagccg atcgcctcgg
     5101 acaacacgcc ggcgagcggc ccgacccaca cacgaccgac ggacgcaaac gccgacagca
     5161 gcgcgtactg cgtcgcggtg aaacgctggt tgcacaggct catcaggaaa gccacgaacg
     5221 cggccgttcc catgccgccc gacaggttct cggcggcgat caccatcaac aggccgccat
     5281 cgatctcggt ggactgcacc agcttcacga aaccccagtc gaaaggcggc agcacgaagc
     5341 cgggaagtgc gcccttgccg ttgaccgcca gccaccagaa cccgaggttg ctgagcatct
     5401 gcagaacgcc gaacagcagc agcgcacgcc acaggcccag cttcagcatc agcaggccgc
     5461 cgagcagcgc gccgccgatg gtcagccaca ggccgatcag cttgttgacg acaccgacct
     5521 cggcagggcc gaaggccatg gacttcagca ggaacggcgt catcagcgag ccggcgaatg
     5581 cgtcaccgag cttgtagagc acgatgaaga tcaggaacgc cgcagcgcct cgctgcgaga
     5641 agtagctgtt cagcccgccc agcagcgtct ggaacttcgc cgcacgtgcc gcccaggcgg
     5701 ccagcggcag cgtgaaggcc agccccagca gcagcgccag caggtcggtc cacttgccct
     5761 tcagcccggc gtccagcgca ctgccgtcga accacggcgc gagcagtgca cgggccaggg
     5821 gcgggccgag gcggtcgctg acgacgaagc cgaccgcaac cgccgccacc agcgccgcga
     5881 agccgagcag gtcctggcgc gcgacgctgg accggtcgcc ggccgcatgc acgcgaggca
     5941 gcaccagcgc cgacagcacc gcggcggccc ccatcaacgc ggccatgaat cggtagacct
     6001 cgggccaggt ccagccgccc gggctgccgg cattcgacgg atcgacccag atcagcacga
     6061 cgcccccgga caggatcatc gccagccggt acccgagcac gttgagcgac gaaccgaggc
     6121 cccgctcgcg ggcggccagc aggtcggtgc ggtaggcatc gatcaccacg tcctgggatg
     6181 cggcgacgaa agccaccagc acggccagca aggcaaatgc gcgtgtggcc tctgccggtg
     6241 gcgtggccgc cagccaccac agtgcgccag ccagggcgag ctgcgtcagc accagccagc
     6301 cccggcgccg acccagccag ggcagctcga accggtccat cagcggcgcc cagaggaact
     6361 tgaacgtgta aggcaggccg accaggctga ggaagccgat cgtcgcgaga tcgaggcctt
     6421 cgaggctcag ccacgcctgc atcgcctggc ctgtcagcgc cagcggcaga cccgaggcga
     6481 agccgagaaa ggtgaccacg gccatccggt gccaggcgcc cgaatcgaat cgagcccggg
     6541 agctcgcaag gggtcgagcg cgttcgaccg gttcgtgatc aggcatgccg gattctagaa
     6601 agcgcccctg gaaccagcgg cagggatccg ggggccagct tcatttcgga ttgcagcgcg
     6661 gcccctgcac cctgcggcga tgtcctgacg gcggccttgc tgcggggcat caccgagaat
     6721 cctcggatga gcgcgatccc gcaagccctt cctccgagct tctcgagcac cgatttccgc
     6781 gccgcgctgg gcaccttcgc cactggcgtg acggtcatca ccgcgcgcga agcgagcggc
     6841 aagccggtgg gtctgacggc caactcgttc aattcggtct cgctgacgcc gccactggtg
     6901 ctctggagct tgtcccgaca ggcctcgacg atgccggcgt tcgcgcgcgg ctcgcactac
     6961 gcgatcaaca tcctcgcagc cgatcagcgc gagatcgccg agcgcttcgc gcgcaaggac
     7021 atcgatcgct tcgatggcat tgcgctgcgc acgggcctga gcggcgcacc ggttctggat
     7081 ggcgtggtgg cggtgttcga gtgcttcaac cgcagccagt acgaggaagg cgaccacgtc
     7141 atcttcgtcg gcgaagtcga gcattgcgct cgacgcgagg gcgcgcagcc gctgatcttc
     7201 cacggtgggc ggtattacac cgagctgccg atctgagcgc acgcaagatc acttccgcgc
     7261 ggccgccctt tccgggtcga tgtcgatgta gcgccagaac tcgcgcatga acgggtggcg
     7321 ctggtagccg atcacccacg gaaacatcag gtcggtggca atgcgatgcg cgtgcacctt
     7381 catcggcatg taagccacca gcagcttgct ggcctcctgc atcagcgcca gccgctccgg
     7441 cgtgtcgccc agcagggtct gctggtcgta aagcttgttg aaggccggca ggtcgaagaa
     7501 cgccaggttg ccctcgccct tctggggacc gtacgccagc gccaggaaac cgtcggcgtc
     7561 tggcgaggag gcgctctgcc ccagccccca catcatgacc ttgcccgcgc gcgcggcctt
     7621 caactgctcg ggccactggc cgatcttgaa ctcgaccttg atgtggaccg cgtccatgta
     7681 cttcttccac agctcgttga gctgtcgcga ggtctggtcg ggcgaggtgc tgtagatcag
     7741 cgtcagcggg ccgccgtcgg ggcgatcgcg ccaaccgtcg ccgttcttgt cgacgtagcc
     7801 gtacaggtcg aggagcgcct tggcgcgggc cgggtcgtag aggctcattt ccgacttcca
     7861 cttcggatcg aatccgaagg tcatcggcgg cagcgtgctt tcggccggca ccgcctggtt
     7921 gcggcgcacg agacggatct cggtctgcgt gtcgtaggcc agcgcgatcg cgcggcgcag
     7981 cgccaccttc tccggcgtgt agccgcccac caccgggtcc tgcatgttga agtaggtgaa
     8041 cgcgatgtcg gacccggggg tgcgcaccat gctgatgccg cgtgcggcga gattcggtgc
     8101 gacccggttg ttcggtatcg ccaggttgga gaactcggcc ggcaggcgct cgatcatgtc
     8161 gtgctcggcg ttcaggaagg ccagccagcg tggctgcgat tcctggacga ccgcgatctc
     8221 gacgcgatcg atcatcggca accgcttgcc tttcatgcgc gcatgaagtg cctgggccgc
     8281 cgggtcatcg gcgggcggtt cggcttcgta gaactgctcg cggaaattcg ggttgcgcgt
     8341 gagcaccatg cgagacgagc gctgccactc ggcgagcacg tagggcccgg tgcccaccgg
     8401 atgctccatg atcttgtcgc cgtaggcctc gaccacttcg cgcgccagcg caccggtgcc
     8461 ggtgaagttg ttgatgaagc gcgggctggg tttgcccagc gtgatgcggt acgtgtagcg
     8521 atcgagcgcg cgcagtccca gcgtgatgcg gtacgtggag cgatcgaggg actgcgcgcg
     8581 ctcg
//
LOCUS       DAOORV010000143         8243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10716_length_8243_cov_8.937103, whole genome shotgun sequence.
ACCESSION   DAOORV010000143 DAOORV010000000
VERSION     DAOORV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10716_length_8243_cov_8.937103"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1490
                     /locus_tag="PKV56_14975"
     CDS             288..1490
                     /locus_tag="PKV56_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOX69161.1"
                     /translation="MNIIRIHCKDEAFAASLPDLFGLRADVERFSIEVPSEVASVLGA
                     GAPVFVGVSGGRDSQALAYRVCAHLDDIGHQGRRFLVHAHLGRVEWLDSLPVCERLAQ
                     RLGVELVVVRRNAGDMMDRWLSRWAGNVARYANLECVKLILPWSTAAQRFCTSELKCQ
                     VIAREMRRRFPEGDVVSAVGIRREESAKRARMPAWKRDERTTRKRGVGHTWNPILGWR
                     RGDVNDYVRIRGDELHEAYRLYGSTRVSCAFCVLGSEHDLRASSNCADNHAIYREMVA
                     LEATSTFSFQSHRWLGDVAPDLLDAGLRARLQEAKERARLRMSAEARLPEHLLFVKGW
                     PTVLPTPGEAALIAGVRRDVAQAVGLQVHHTDRDAVLVRYRELMTVARAKAVANDLPL
                     HTEEGLQT"
     gene            1487..2347
                     /locus_tag="PKV56_14980"
     CDS             1487..2347
                     /locus_tag="PKV56_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69162.1"
                     /translation="MNSTTLAPRAGAVPEPLVDIASISSRLFWLVRNSMSGAAAGLHL
                     PTTVFTRFSDAVAARDAIDETPPLLDHPQGLCRLRDDVIVDGVGPGRVNWIHPSGDVR
                     VELARHPGSAEFYPAAAIRQAVAPRPRFEHEVVPIGLREANLVVERLHRRLGKVQGHK
                     FAVGLRRVADQELTGVAVCARPVARHLDDGLTAEVRRVAVDPSVSNGCTKLLGAIAKA
                     ASAMGYRRLVTYTAEDESGASLYAAGWDPIGRSAGGHWGSSRRPRRPGPQEGRKVVWV
                     KVLRGEGRER"
     gene            2417..4087
                     /locus_tag="PKV56_14985"
     CDS             2417..4087
                     /locus_tag="PKV56_14985"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOX69163.1"
                     /translation="MQQLIDLRHETPEAISLQAIGSFMQAGRPAIVLFSGGKDSSVLL
                     NLALTAASEQAKQGRKALVVIAHSDVGVENPEIQRLVSGEIAKAARFAREHGVTAVVR
                     VATPAFWDTFPVRVIGGRALPTFPDSRRDCQTDLKRLPNERELALIERDLVAKGWPQP
                     VLMTGVRRDESAVRAASVKGRGEKAVSLWTDREGRLRLSPLLDWAVDDVWQYLGLANA
                     GVIPAYSRFEETMQTYQAAGGSSCVVVADAEMQKHSKPCSSRFGCWVCTAVREDRSLR
                     QMIETDRDRYGYMAPLAALRDFIAFTQYDWSRRQYVGRSIVDGFIEVGADTYSPAMLA
                     ELLRYTLSAQKLSGVQIVSAAQLIAIDARWSQYAIAEPFSALRIWKQVEDGAVWTPPS
                     LKPYRKTAVPKLGKIYVGDWNDDVTSALEITGLRNPAWEDFAETCGPGLRTLASGRTV
                     IDVEGDSEIDEEGAWLFLDFELDRMLTEHRAKDGYWTAGYETYLSFGLVQPAKGQSAR
                     VDEILRRSQWRQRHGLHGQPSLESLRTRLTVRYPSQGELFEELHAVLA"
     gene            4294..4593
                     /locus_tag="PKV56_14990"
     CDS             4294..4593
                     /locus_tag="PKV56_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69164.1"
                     /translation="MVEDKSKLVARGAHTHEIRDVSGEWIWLGDELSMQVIFRNLTGE
                     NFAGRPATAYADYLSWMRHFTAASWPGRSDPLGQGETIELAPINGTPIARHKFGS"
     gene            4612..5262
                     /locus_tag="PKV56_14995"
     CDS             4612..5262
                     /locus_tag="PKV56_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOX69165.1"
                     /translation="MNAEELARTLIAALPKGAPGLFNPWADVCGDDLGSNGPDAKLRR
                     LQQHLDCDAEFILCGEAAGWQGMRHTGLAFTSERHVVDGTVPRLGGAPGRLTSRDRPF
                     AEPSATLVWRALYVQGIAERVVMWNALPMHPHERGDPRSNRTPNVAELTHGRAPLKLL
                     LAAFPRAAVVAVGKKAAEQLVQLGMTPAAAIRHPANGGARAFAQGLEAFVETRRRK"
     gene            5590..6300
                     /locus_tag="PKV56_15000"
     CDS             5590..6300
                     /locus_tag="PKV56_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69166.1"
                     /translation="MNDLGTDSVQSAIAALQDKGYGVRRTPGPQQREASYAFVLSRPG
                     MARALFGPARSSVFAAWASAQEHAEQGETPASTQRLTPFYSAELPESAFDASALAHRF
                     GVDVESAERQVQQLRCQSIFLSETHQVNVQLLRCPFGTHLGDVAWLSIKRRDREVIRD
                     WRELQAIKNAIVGPEHEGFELYPAESRLVDTANQFHLFVFMDTKVRMPVGFGEREVSG
                     ADAAAAVGATQRELPESA"
     gene            6300..7289
                     /locus_tag="PKV56_15005"
     CDS             6300..7289
                     /locus_tag="PKV56_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69167.1"
                     /translation="MAFEVLEAPVERDAQGAGRKAGPILRVGIPTTGGRILKAAKDAG
                     YSVLFSTNAFMVRDGNGDVVGVRRPDPKQFAGLDAALDSAGFVAMAKYRGFPWTIEQY
                     LDLVESWPWSWYASWDQCVEPEIASSKIDVFFRLAETCRLLSEVRMRARDRGLADPMP
                     VVQGWEVDHYRWSLDHLPLAAWPSLLGVGSMCRRHVHGPNGILAIVEALDRVLPSGVG
                     LHLFGVKGTALRFLSDHPRISSVDSMAWEFAARRQVPIGRTVDKRIDVMHAWVRRNAS
                     QLELPKAPYELQLFDEPSDEASAELQEWLELVVDNQTDAVSAWHHCMRTWVGG"
     gene            complement(7317..7946)
                     /locus_tag="PKV56_15010"
     CDS             complement(7317..7946)
                     /locus_tag="PKV56_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69168.1"
                     /translation="MRHCDLLLSLLTPFWATCAIACAVPQDERPHSQRLADAPLAFIG
                     TVTSMSGLRVTFAVHHAINGSPGPSTTVEVLSPSTCSIAFAVGQRWLYAGPATSQPSV
                     LLLAKARSAPATDFGRLRRVDDARAQLPAAWQTCTANAQCEPVPIGCQRTSANAANVA
                     AARAQSTKLLGDHRAMDCATPPVEIQPLASPQCVASRCGHWFLDLRLGV"
BASE COUNT         1382 a         2557 c         2948 g         1356 t
ORIGIN      
        1 agccgttgac ggctctcgtt gaaggctgta ccagcgacga tcgccagcag gaagccctgc
       61 aagtttgcca ctcggtagcg gccctgggta ggggtcgcgc gaggccttga agccggcgca
      121 ggttcatgtg tccggcttgt gcctgatgac acgggctgga gcgctccggc ggtgcatcgg
      181 cagctgccga tgaggccctc ggacagcgtc tacgggctcg tgcgtccggc aagggggcgt
      241 ggggctgtgc cccttgaaca caaggaggtg cctcatagcc tgagtgcgtg aacatcatca
      301 ggatccactg caaagacgaa gccttcgcgg cgagcctgcc ggacctgttc ggcttgaggg
      361 cggatgtcga acggttctcc atcgaggtgc cttccgaggt cgcgagtgtc ctgggtgcgg
      421 gggcaccggt gttcgtgggc gtgagcggag ggcgcgactc tcaggcgctg gcctatcggg
      481 tgtgtgcgca cctcgacgac atcggacacc agggccggcg cttcctcgtt catgcccact
      541 tgggccgcgt ggagtggctg gacagcctgc ctgtctgcga gcggctggcg cagcgccttg
      601 gtgtggaact cgtcgtcgta cgccgcaacg ccggcgacat gatggatcga tggctgtcgc
      661 gctgggccgg taacgtcgcg cgctacgcga acctcgagtg cgtgaagctg atcctgccgt
      721 ggagcaccgc cgcacagcgc ttctgcacgt ccgagctcaa gtgccaggtc atcgcccggg
      781 aaatgcgccg gcgctttcca gagggtgatg tcgtgtccgc cgtgggcatc cggcgcgaag
      841 agagcgccaa gagggcgcgc atgccggcct ggaagcggga cgagcgcacg acacgcaagc
      901 gcggcgtagg ccacacctgg aaccccatcc ttggctggcg gcgcggcgac gtcaacgact
      961 acgtgcgcat ccggggcgac gagctccatg aggcctaccg gctctatggc agcacgaggg
     1021 tctcgtgcgc gttctgcgtg ctcggctcgg agcatgacct tcgggcgtcg agcaactgcg
     1081 ccgacaacca tgcgatctac cgcgagatgg tggcgctgga agccacgagc accttctcgt
     1141 tccagagcca ccgttggctc ggcgacgtgg ctccggacct gctcgatgcg ggtcttcgcg
     1201 cgcggctgca ggaggccaag gagcgtgccc ggttgcggat gagtgctgag gccaggctgc
     1261 cggaacacct gctcttcgtg aagggctggc ccacggtcct gcccacgccg ggcgaagcgg
     1321 cactgatcgc cggggtccgt cgcgatgtcg cgcaggccgt cggcctgcag gtgcatcaca
     1381 ccgaccggga cgcagtgctg gtgcgctacc gcgaactgat gacggtggcc agagcgaagg
     1441 cagtggccaa cgacttgccg ctgcacaccg aagaaggact gcaaacatga actcgaccac
     1501 cctggcgccg agagccggcg ccgttcccga accgctggtg gacatcgctt cgatctcgtc
     1561 gcggctgttc tggctggttc gcaactcgat gtctggtgcc gcggcgggcc tgcatttgcc
     1621 gacgacggtg ttcacccggt tcagcgacgc cgtggccgca cgcgatgcca tcgacgagac
     1681 cccgcctctg ttggaccacc ctcagggcct gtgccggctg cgcgacgacg tgatcgtcga
     1741 cggtgtgggg ccgggtcgtg tcaactggat ccatccgagc ggcgatgtgc gcgtcgagct
     1801 cgcgcggcat ccgggcagcg cggagttcta ccccgccgca gcgatcaggc aggcggtcgc
     1861 gcccaggccg cgcttcgagc acgaggtggt acccatcggc cttcgtgagg cgaacctggt
     1921 ggtggagcgg ctgcaccgca ggctgggcaa ggtccaggga cacaagttcg ccgtcggtct
     1981 gcgtcgtgtc gcggatcagg agttgactgg cgtggcggta tgcgcgcggc cggtggcacg
     2041 gcacctggac gatggcctca cggccgaggt tcgccgggtg gccgtcgacc catcggtgag
     2101 caacggctgc accaagttgc tcggggccat cgcgaaggct gcctccgcga tggggtatcg
     2161 gcgcctcgtg acctacacgg cagaagacga gagcggcgcc agcctctatg ccgccggctg
     2221 ggaccccatc gggcgcagcg ccggcgggca ctggggatcg tccaggcggc cgcgccgtcc
     2281 aggcccccag gagggacgca aggttgtgtg ggtgaaggtg ctgcgcggcg agggacgcga
     2341 acgctgaacg ttgctcgatt ccgtgggctt gaaggggcgg acgtgcgcgg gatcctgatg
     2401 ttctccagga gcgcgcatgc agcaactcat cgaccttcgc cacgagacgc cagaggccat
     2461 ctcactgcag gcaatcggct cgttcatgca ggccgggcgg ccggcgatcg tgctgttcag
     2521 cgggggcaag gattcgtccg tgctgctcaa cctcgcgctg accgccgcca gtgaacaggc
     2581 caagcagggc cggaaagccc tggtcgtcat cgctcacagc gacgtcggcg tcgagaaccc
     2641 ggagatccag cgccttgtca gcggcgagat tgccaaggcg gctcggttcg cccgagagca
     2701 cggcgtgacc gccgttgtgc gggtggcaac gcctgccttc tgggacacgt tccccgtgcg
     2761 ggtgatcggc gggcgcgcgc ttcccacttt tccggactca cgacgggact gccagactga
     2821 cctgaaacga ctgccgaacg agcgcgagct ggccctcatc gaacgtgatc tggtcgccaa
     2881 gggctggcct cagccggtgc ttatgaccgg tgtgcggcgc gacgagtcgg ccgtgcgtgc
     2941 agcgagcgtg aagggacgtg gggagaaggc ggtgtcgctc tggacggaca gggaagggcg
     3001 ccttcggctg agcccgctgt tggactgggc cgtcgacgat gtctggcagt acctcggcct
     3061 tgccaatgcg ggtgtgatcc cggcctacag ccgcttcgag gagacgatgc agacctacca
     3121 ggccgccgga ggcagcagct gcgtcgtggt ggcggacgcg gagatgcaga agcactccaa
     3181 gccttgctcc agccgcttcg gctgctgggt ctgcaccgca gtccgggagg accggtcgct
     3241 gcgccagatg atcgagaccg atcgtgatcg ctacggctac atggcgcccc tggccgcgct
     3301 tcgggacttc attgcattca cccagtacga ctggtcacgc cggcagtacg tcgggcgcag
     3361 catcgtggac ggattcatcg aggtcggcgc cgacacatac tcgcccgcga tgctcgccga
     3421 actgcttcgg tacaccttga gcgcccagaa gctgtccggt gtgcagatcg tcagcgcagc
     3481 ccagctgatc gcgattgacg cgcgctggtc gcagtatgcg atcgccgaac ctttcagcgc
     3541 gctgcggatc tggaagcagg tcgaagacgg tgcggtctgg acgccgccct cgctcaagcc
     3601 ctaccggaag acggcggtgc cgaagctcgg gaagatctac gtcggtgact ggaacgacga
     3661 cgtgacctca gcgctggaga tcaccggtct gcgcaatccg gcatgggagg acttcgcaga
     3721 gacctgcggc ccaggccttc gcaccctcgc gagcggacgg acggtcatcg atgtcgaggg
     3781 cgacagcgag atcgacgagg agggtgcctg gctgttcctg gacttcgagc tcgaccgcat
     3841 gctcaccgag caccgcgcga aagatggcta ctggacagcc ggttatgaaa cctacctatc
     3901 gttcggcctg gtccagccgg ccaagggcca gtcggctcgg gtcgatgaga tcctgcggcg
     3961 atcgcaatgg cggcagcgcc atggcctgca cgggcagccc tccctcgagt ccctgcgcac
     4021 tcgcctgacc gtgcggtatc ccagccaggg cgagctgttc gaggagctcc acgcggtgct
     4081 ggcatgaggt tgctcgacct cgcgggcggc accccctttg tggggcgctc gaaggggcgc
     4141 ccgccgaggg cggaaggcgt tgcgcagcgc aggcgctagg ccgacctcag cggaggctag
     4201 gtggtcatgc tcgctcccca atgcgggtgc ctacgcagtg gcgcttgaac gcggggcggg
     4261 gcccggcaga ctggtcccga aaggatcgaa gtcatggtcg aagacaagtc gaagctggtg
     4321 gccaggggcg cccacacaca cgagatccgg gatgtaagcg gggagtggat ctggctgggt
     4381 gacgagctga gcatgcaggt gatcttcagg aacctgacgg gggaaaactt cgcgggcagg
     4441 cctgcgacgg cctatgcgga ctacctgagc tggatgcggc acttcacggc ggcgtcctgg
     4501 ccgggacgaa gtgaccccct cggacagggc gagacaatcg aactcgcacc catcaacggg
     4561 acgccgatcg ctcggcacaa gttcgggtcg tagagtgaac gggaggggac catgaatgcc
     4621 gaggaactgg ctcggacgct gatcgctgcg ctgccgaagg gcgcgcctgg actcttcaat
     4681 ccgtgggccg acgtgtgcgg agacgatctc ggatcgaatg ggccggacgc caagctgcga
     4741 cgtctgcagc aacatctgga ctgcgacgcg gagttcatcc tctgcggcga ggcggcgggt
     4801 tggcaaggga tgcgacacac gggcctggcc ttcacgagcg agcggcacgt ggtggatggc
     4861 acggtgcccc gcctgggtgg ggcgcctgga agactgacct cccgggacag accgtttgcc
     4921 gagccctcgg ccaccttggt ttggcgcgcg ctgtacgtcc agggcattgc ggagcgggtg
     4981 gtgatgtgga atgcgctgcc gatgcacccc cacgagcgtg gcgacccgcg gtccaatcga
     5041 acgccgaacg tcgcagagct cactcacggc cgagcccctc tcaagctcct gttggctgcg
     5101 tttccacgtg cggcagtggt tgcggtgggc aagaaggcgg ctgagcaact ggtccagctg
     5161 ggcatgacgc cagcggctgc gatccggcat cccgccaatg gtggggcacg ggcgttcgcg
     5221 caaggcctgg aggcgttcgt cgagacgcgg cggaggaagt gaacgtgccc gacgatcaga
     5281 cggccgccgg cgggggcacc cggtactggc tgctccatga acttccgatc ggccatccgc
     5341 ttcgcagcca accgctcgga ccgatggcag cccaatgccg gaaccttcat tccaaggagt
     5401 ggcgtgacct tgcgcgctgg cacatagccc gctgcgcata cgacgacctt ggtgaagcct
     5461 ggacggcgac aagcgagttt cgggtcaggg cgccctgagc gttgcggcct cggcaagacg
     5521 cagaccttga agatgaggta gccggcgaga tcctgaaggt ccagtcaacg gaccttgcag
     5581 gcacctctca tgaatgacct cggcacggac tcggtccaga gcgcaatcgc tgcactccag
     5641 gacaaggggt acggtgtccg ccgaacccct ggcccgcaac agcgcgaagc cagctatgcc
     5701 tttgtgttga gccggccggg gatggcgagg gcgctcttcg gcccggcgcg ctcgagcgtc
     5761 tttgcagcgt gggcatcggc gcaggagcat gcagaacagg gcgagacccc ggcgtcgacg
     5821 cagcggctga cgccgttcta ttcggcggag ttgcccgaaa gcgccttcga cgcgtcggcc
     5881 ctcgcgcacc ggttcggtgt cgatgtcgag tcggccgagc gccaggtgca gcagctgcgc
     5941 tgccagtcca tcttcctgag cgagacccat caggtcaacg tgcagcttct gcgctgcccc
     6001 ttcgggacgc acctcggcga tgtggcctgg ctttccatca agcgtcgcga tcgcgaggtg
     6061 atccgggact ggcgcgaact gcaagccatc aagaacgcca tcgtcggtcc cgagcacgaa
     6121 ggcttcgagc tctaccccgc cgagtcgcgc ctggtcgata ccgcgaacca gttccacctc
     6181 ttcgtgttca tggacacgaa ggtgcgcatg cccgtgggct tcggcgagcg ggaggtgtca
     6241 ggcgcagatg cggccgcggc ggtcggcgcg acgcagcgcg agttgcctga aagcgcgtga
     6301 tggcgttcga ggtattggag gcgccggtcg agcgcgacgc ccagggcgcc ggccgcaagg
     6361 ccggccccat cttgcgggtc ggcatcccga ccaccggcgg caggatcctc aaggcagcca
     6421 aggatgcggg ctacagcgtc ctgttcagca ccaacgcctt catggtgcgc gacggaaatg
     6481 gggatgttgt gggtgttcgc cgacccgatc ccaaacagtt tgccggcctg gacgccgcct
     6541 tggacagcgc cggcttcgtg gcgatggcga agtaccgcgg cttcccatgg acgatcgagc
     6601 agtacctgga cctggtggag agctggccat ggagctggta tgccagctgg gaccagtgcg
     6661 tcgagcccga gatcgcttcg tccaagatcg acgtgttctt ccggctggcc gagacctgcc
     6721 ggctgttgtc cgaggtgcgc atgcgggcac gggatcgtgg tctagccgac ccgatgcctg
     6781 ttgtccaggg ctgggaggtc gaccactatc ggtggtcgct ggatcacctg ccgctggctg
     6841 catggccgtc gctcctgggt gtgggttcga tgtgccggcg gcatgtgcac ggaccgaacg
     6901 gaatcctggc gatcgtggag gcgctcgacc gcgtcctgcc gtctggggtt ggcctgcact
     6961 tgttcggagt gaaggggacg gcactgcgct tcctcagcga tcatccacgc atctccagtg
     7021 tcgactcgat ggcctgggag ttcgcggcac gtcgccaggt gccgatcggc cggacggtgg
     7081 acaagcggat cgacgtgatg catgcctggg tgcgcaggaa cgcctcgcag ttggaactgc
     7141 caaaggcgcc gtacgaactt cagctgtttg acgaacccag tgatgaggcc tcagcagaat
     7201 tgcaggaatg gttggagctg gtggtggaca accagaccga tgcggtcagc gcctggcacc
     7261 actgcatgag gacctgggtc ggtgggtgag cgccacggcg acgaggtgcg ctgaggctac
     7321 acgcccaagc gaagatccag gaaccagtgg ccacatcggg acgcgacgca ctgtggtgag
     7381 gcaagcggtt ggatctccac cggcggggtc gcacagtcca tcgcgcgatg gtcgccaagg
     7441 agcttcgtcg actgagctcg agcggcggca acattggccg cgttggccga ggtccgctga
     7501 cacccgatcg ggacgggttc gcactgagca tttgccgtgc aggtctgcca ggcggccggg
     7561 agctgcgccc gagcgtcgtc gacgcgccgc agcctgccga agtcggtcgc tggggcactg
     7621 cgtgctttgg caaggagcag gaccgagggc tggctcgtgg cgggccccgc atagagccac
     7681 cgctgtccaa cggcgaaggc gatggagcag gtggatggcg acagcacttc gacggtggtt
     7741 gacggtcccg gtgaaccatt gatggcgtgg tggaccgcaa aggtgacgcg cagtcccgac
     7801 atggacgtga cggtgccaat gaaggccagc ggtgcatcgg ccaggcgttg cgagtgcggg
     7861 cgctcgtcct gcggcacggc gcatgcgatg gcgcatgtcg cccagaacgg agtgagcaag
     7921 gacagcagca agtcgcaatg gcgcatcgca ccctctattg aagttgctgg gcatcgtcgc
     7981 aaagcgagtg gatatcgtcc aatcgaaaca ccggtcgggg ccccatggat ttcggcagtc
     8041 gaagtcgctt gccggtggcg ccgccttcaa gcaaacaatt gaggtcgccg gttgccccgc
     8101 catcaatgac ctagttttac tgtgtcacaa gagaagcctt gcgtttgcgc gcaggcaaca
     8161 cgggcagcgt ggcagcgtct tgagtaaggc tgcggcgata cgcagccgca agctcgtgat
     8221 ggacgatggg acgtggcgct gcg
//
LOCUS       DAOORV010000144         8191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10804_length_8191_cov_6.944813, whole genome shotgun sequence.
ACCESSION   DAOORV010000144 DAOORV010000000
VERSION     DAOORV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10804_length_8191_cov_6.944813"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="ssb"
                     /locus_tag="PKV56_15015"
     CDS             <1..378
                     /gene="ssb"
                     /locus_tag="PKV56_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOX69169.1"
                     /translation="ETEWHRVVFYDRLAEIAGEYLKKGRPVYVEGRLKTRKWQDKEGK
                     DVYTTEVIAENMQLLGGREGMGGGDVGGDSGYDRGDNRGEGRSDARPAQAARSAPPSR
                     PAPSKPAPKSSTGFDDMDDDIPF"
     gene            complement(421..1749)
                     /locus_tag="PKV56_15020"
     CDS             complement(421..1749)
                     /locus_tag="PKV56_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOX69170.1"
                     /translation="MSRLGWRLLAAIFAAVAALLATVVALNLRGEGNLSAEIDSASIQ
                     VPGPGSDPDALARGAYLARAGNCEACHTARGGAPYSGGRAIDTPFGTVYSTNLTPDPD
                     TGLGHWSAPQFWRAMHHGRGADGRLLVPAFPYTSTTLVTRADSDALYAYLRSLPSVQR
                     AARPHELRFPFNTQAALAVWRALYFKPGVFEPDAARSADWNRGAYLVQGLGHCNACHA
                     KRNALGATEGPLDLGGGLIPIQNWYAPSLTDPYEAGVASWSATDIVALLKNGVVDRAG
                     ALVTASGPMGEVVLRSTQHLSEADLGAMAAYLKTLGATHADAAEPAAQAASAPPSAAG
                     RGATLYADHCAACHGDQGAGIAGAYPPLAGNRAVTMAVPANLVRIVLEGGFAPSTAGN
                     PRPYGMPPFSPILNNDDIALLLSHLRGSWGNRAGEVSAADVNRYRGSGAR"
     gene            complement(1746..2462)
                     /locus_tag="PKV56_15025"
     CDS             complement(1746..2462)
                     /locus_tag="PKV56_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HOX69171.1"
                     /translation="MCVLGACLSVSFGASATPAFEDTLAQRLQACTGCHGEQGRASPG
                     GYMPRLAGKPAGYLFHQLLNFRDGRRGYGPMTSLVDPLSDDYLEEIAAHFASLDLTYP
                     PPQPATASAATLERGERLVRQGDPAAQVPACSRCHGDAMMGVAPAIPGLLGLPRDYVN
                     AQLGAWRTGKRRAQAPDCMAHVAKQLSLSDIHAVSQWLAMQPVPPGAKPLAAWPAGQR
                     LPVACGGVGVDAVEASGASR"
     gene            complement(2669..3313)
                     /locus_tag="PKV56_15030"
     CDS             complement(2669..3313)
                     /locus_tag="PKV56_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOX69172.1"
                     /translation="MYLPAHFEETRLEVLHDLMRGHPLGLLVTQGHGEPPELEANALP
                     FHFDPARGAHGTLVAHVARANPLWRETRADIEVLVVFQGPQAYISPGWYATKAETGKV
                     VPTWNYILVEARGRLRVRDDAAWVRALVSGLTDIHEAAQARPWKVADAPADYIAETQR
                     AIVGIEIELTALKGKWKMSQNRSAADLAGVARGLATSGQEDVAVWVDPGPRAPG"
     gene            complement(3518..4648)
                     /gene="mnmA"
                     /locus_tag="PKV56_15035"
     CDS             complement(3518..4648)
                     /gene="mnmA"
                     /locus_tag="PKV56_15035"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOX69173.1"
                     /translation="MTVRKRIVVGLSGGVDSAVSAHLLKQQGHEVIGIFMKNWEDDDD
                     SEHCSSRQDFLDAASVADVLGIEIEHVNFAADYRDRVFADFLREYQAGRTPNPDVLCN
                     AEIKFKAFLDHAMRLGAEAIATGHYARVRQVDGFFQLLKGLDPLKDQSYFLHRLNQAQ
                     LSKTLFPVGELPKTEVRRIAAEIGLPNAKKKDSTGICFIGERPFREFLNRYLAHTPGP
                     IKDDRGRIIGEHVGLSFYTLGQRKGIGIGGLKARGAARGGSDHAPWFTARKDMGTNTL
                     YVVQGHDHPWLLSSALVADDASWTASFPPPAGSLAAKTRYRQADAACLLAPPASPESR
                     PQAFELRFATPQWAVTPGQSAVVYDGEVCLGGGVIAEVQVDR"
     gene            complement(4645..5136)
                     /locus_tag="PKV56_15040"
     CDS             complement(4645..5136)
                     /locus_tag="PKV56_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOX69174.1"
                     /translation="MNERWKPNVTVAAVIERDGRFLLVEEHTPEGLRLNNPAGHLDQA
                     ESPQEGVVREVLEETACVFTPQSIVGVYLGRFQRPSRGEDVTYFRIAYAGTVGDPLPG
                     RALDDGIVRTVWMTLEELRECTPRHRSSMVLQSIEDHVAGRRHPLELISTEASVYSPA
                     IKT"
     gene            5299..6432
                     /locus_tag="PKV56_15045"
     CDS             5299..6432
                     /locus_tag="PKV56_15045"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOX69175.1"
                     /translation="MLIGIPLETASGETRVAVTPETARKLTGQGHTVRIQSGAGVAAS
                     ATDEAYSAAGAEITDAAGAFGCELVLKVRAPSAAELPMLKAGSALVGMLNPFDGEGLQ
                     RLATAGVTGFALEAAPRTTRAQSMDVLSSQANIAGYKAVMIAADKYQRFFPMLMTAAG
                     TVKAARVVILGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIEVSYDTP
                     EEKEAAVGVGGYAKPMPQSWLDRQKIEVAKRVAAADVVISTALIPGRAAPTLVTEEMV
                     KAMKPGSVIVDIAAGKGPNGVGGNCPLSEADKTVVKHGVTLVGETNLPALVAADSSAL
                     YARNVLDFLKLVITKEGTFNVPMDDDIVAACLMTQGGEVKRTS"
     gene            6503..6865
                     /locus_tag="PKV56_15050"
     CDS             6503..6865
                     /locus_tag="PKV56_15050"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOX69176.1"
                     /translation="MNRVMLRAKLHRATVTEADLHYEGSCGIDSSLLEQADMREFERI
                     ELYNVNNGERFSTYAIKAAPGSGVISLNGAAARRAQVGDLLIICTYATMTEAEAAAYK
                     PKVLLLDECNRVKEVRKF"
     gene            6886..7206
                     /locus_tag="PKV56_15055"
     CDS             6886..7206
                     /locus_tag="PKV56_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOX69177.1"
                     /translation="MDIISPTITNLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTETTLGQTMGVLAVALAAVNVFGGFLVTRRMLEMFKKKEKKAVAP
                     ETAK"
     gene            7203..>8191
                     /locus_tag="PKV56_15060"
     CDS             7203..>8191
                     /locus_tag="PKV56_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOX69178.1"
                     /translation="MSLNLVTLLYLVASICFIQALKGLSHPTTSIRGNLFGMVGMTIA
                     VLTTAALIVKLAGASTASASTGGLLYVIGALVVGGAVGAVMAKRVEMTKMPELVAFMH
                     SMIGLAAVFIAVAAVAEPYAFNIVAKGEPIPTGNRLELFLGAAIGAITFSGSVIAFGK
                     LSGKYKFRLFQGAPVQFAGQHKLNLILGLTTVGLGLLFMFTGSWTAFFAMLALAFVMG
                     VLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMC
                     KAMNRSFFNVIGGGFGGEATSAGGAKAEARPVKSGSADDAAFVLGNAETVVIVP"
BASE COUNT         1403 a         2754 c         2746 g         1288 t
ORIGIN      
        1 gaaaccgaat ggcaccgcgt cgtcttctac gaccgcctgg ccgagatcgc cggcgagtac
       61 ctgaagaagg ggcgtccggt ctacgtcgag ggccgcctga agacccgcaa gtggcaggac
      121 aaggaaggca aggacgtcta cacgaccgaa gtgattgccg agaacatgca gctgctgggc
      181 ggccgtgaag gcatgggcgg gggcgatgtg ggcggggatt ccggctacga ccgcggtgac
      241 aaccggggag agggccgcag tgatgcgcgc ccggcccagg cagcacgcag cgccccgccg
      301 agccgcccgg ccccctcgaa gccggctccg aagtcgtcga ccggcttcga cgacatggac
      361 gacgacattc ccttctgagt caccacaccg cccggcgact gcaccaccgc gcgcgctgat
      421 tcagcgcgcg ccgctgcccc tgtagcggtt cacgtccgcg gccgacacct caccagcgcg
      481 gttgccccag ctgccgcgca gatgcgacag cagcaaggcg atgtcgtcgt tgttcaggat
      541 cggcgagaac ggtggcatgc catagggccg ggggttcccg gctgtcgacg gagcgaatcc
      601 cccttccagc acgatgcgca cgagattcgc aggcaccgcc atcgtcaccg cgcggttgcc
      661 agccagcggg ggataggccc cggcaattcc ggcgccctga tcgccatgac aggcggcaca
      721 gtgatcggcg tacagcgtcg caccgcgtcc cgccgctgat ggcggtgcag atgcggcctg
      781 agccgccggc tccgccgcgt cggcgtgcgt tgctccgagc gtcttcagat aggcagccat
      841 cgcgccgaga tcggcttcgc tcaggtgctg ggtgctgcgc agcacgacct ctcccatcgg
      901 acccgacgcg gtgaccagcg caccggctcg gtccaccaca ccgttcttca gcagcgcgac
      961 gatgtcggtg gcggaccagc ttgccacgcc agcctcgtat ggatcggtca gcgacggcgc
     1021 ataccagttc tggatcggta tcaggccgcc gcccaggtcg agcggcccct ctgtcgcgcc
     1081 gagcgcgttg cgcttggcgt ggcaggcgtt gcagtggccc agtccctgga cgaggtaggc
     1141 cccgcggttc caatcggccg atcgggccgc atccggctcg aacacgcccg gcttgaagta
     1201 cagcgcgcgc cagactgcca gcgcggcctg cgtgttgaac ggaaacctca gctcgtgcgg
     1261 cctggcggcc cgctgtaccg agggcaggct gcgcaggtat gcgtacagcg cgtccgaatc
     1321 ggcgcgggtg accagcgtgg tgctggtgta agggaatgcc ggcaccagca ggcgcccgtc
     1381 ggccccgcgg ccatggtgca tggcgcgcca gaactggggc gccgaccagt gcccgagacc
     1441 ggtgtcggga tcgggggtga ggttggtgct gtagaccgtg ccgaacgggg tgtcgatggc
     1501 gcgtccacct gaataaggcg caccgccgcg cgccgtgtgg cacgcctcgc agttgccggc
     1561 gcgcgccagg taggcgcccc gcgccagcgc atcggggtct gaccccggac caggcacctg
     1621 tatcgaggcg ctgtcgattt cggcactcag gttgccctcg ccgcgcaggt tgagcgcaac
     1681 aaccgttgcc agcagtgcag ccacagcggc gaatatcgcg gccagaagcc gccagcccag
     1741 gcggctcatc gcgacgctcc gcttgcttca acggcatcca cacccacgcc accacaggcc
     1801 accggcagcc tttggcccgc aggccacgcg gccagcggct tggcgccggg cggcaccggt
     1861 tgcatggcca gccactgcga caccgcgtgg atgtcgctca gactcagctg cttggccaca
     1921 tgcgccatgc aatcgggcgc ctgcgcacgc cgcttgcccg tgcgccatgc accgagctgc
     1981 gcgttcacgt agtcgcgtgg cagtcccagc aggcccggga tagccggcgc aacacccatc
     2041 atcgcgtcgc cgtggcaccg ggaacaggcc ggcacctgcg ctgctggatc gccctggcgc
     2101 accagccgct ctccgcgctc cagtgtcgcg gcgctggcgg tcgcgggctg cggtggcgga
     2161 taagtcaagt ccagcgaggc gaaatgcgcc gcgatctcct ccaggtaatc atcactgagc
     2221 ggatcgacca gtgaggtcat cggcccgtag ccgcgacggc catcccggaa attgagcaac
     2281 tggtggaaca ggtaaccggc aggcttgccg gccaggcgag gcatgtaacc gccgggtgag
     2341 gcgcgcccct gctcgccatg gcagcccgtg caggcctgca gccgctgagc cagcgtgtcc
     2401 tcgaacgccg gagtggccga cgccccaaac gacaccgaca gacacgcacc aagaacgcac
     2461 atcgcggcgg ccaccctggt cttgcaaccc cgcgccaggg ttcgaaaaac ggtcaatgtc
     2521 gtgtggtgca tggcgggaat cggacgggtt atccctgatt atccgatgga ggccggctcc
     2581 ccctccatcc accactcagc ggaatccggc tgtgctgcac ggtgatgggg cggagcgaat
     2641 gagcagcgcc ccaaagcgga ccgcccgctc agcctggggc tcttggtccc ggatcgaccc
     2701 agacggcgac gtcttcctgc cccgatgtcg ccaaaccccg tgccacccca gcaaggtcgg
     2761 ctgctgatcg gttctggctc atcttccact tgcccttcaa ggcagtgagc tcgatctcga
     2821 tgccgacgat cgcgcgttgc gtctcggcga tgtagtcggc aggcgcatcg gccaccttcc
     2881 agggtcgcgc ctgcgcagcc tcatggatgt ctgtcaggcc agacaccagc gcacggaccc
     2941 aggccgcatc gtcacgcacc cgcaaccggc cacgcgcctc gacgaggatg tagttccagg
     3001 tcggcaccac cttgcccgtc tcggccttcg tcgcgtacca gcccggcgag atgtaggcct
     3061 gcggcccctg gaacacaacc agcacctcga tgtcggcgcg cgtctctcgc caaagcggat
     3121 tggcgcgggc cacgtgggcc accagagtgc catgggcccc ccgcgccgga tcgaagtgga
     3181 aaggcagcgc gttggcctcg agctccggtg gctccccgtg cccctgggtc acgagcaacc
     3241 cgagcggatg accccgcatc agatcgtgga gcacctcaag gcgcgtctct tcgaaatgag
     3301 cgggcaggta catgacgggc gagatcgggt ggagaagacc gcagagattg ccacagccgc
     3361 aacggaggct tgcggatcaa actggccttt ctcatctgcg cctcggacgc cccaggccag
     3421 ctagcagacg ccgcctggga ctgtagggga agcgagcggg ccgacagcaa cgcaggcagc
     3481 accttcaacc agtgcgggtc tgcctgcggc ccgcgactca gcggtcgacc tggacctcgg
     3541 cgatgacacc accgccgagg cacacctcgc cgtcgtacac cacggccgac tgccccggtg
     3601 tgacagccca ctgcggcgtg gcaaaacgca gttcgaaggc ctgcggacgg gactcgggcg
     3661 acgccggcgg tgccagcagg cacgcggcat cggcctgtcg gtagcgcgtc ttggccgcca
     3721 gcgaaccggc gggaggcggg aagctggcgg tccagcttgc atcgtcggcc accagtgccg
     3781 aagacagcag ccacgggtgg tcgtgcccct gcaccacata gagcgtgttg gtgcccatgt
     3841 ccttgcgggc ggtgaaccac ggtgcatggt cactgccacc tcgcgcagca ccgcgagcct
     3901 tcagcccgcc gatcccgatg cccttgcgct gacccagcgt gtagaagctc aggccgacgt
     3961 gttcgccaat gattcggcca cggtcgtcct tgatcggacc cggcgtgtga gccaggtaac
     4021 gattcaggaa ttcgcggaac ggccgctcgc cgatgaagca gatgccggtg ctgtccttct
     4081 tcttcgcgtt tggcagcccg atctcggctg caatgcgccg gacctcggtc ttgggcagct
     4141 cgccgaccgg aaacagcgtc ttcgagagct gcgcctgatt caggcgatgc aggaaatagc
     4201 tctgatcctt cagcgggtcg agccccttga gcagctggaa aaagccatcg acctggcgaa
     4261 cgcgcgcata gtgaccggtg gcgatggcct ccgcgcccag ccgcatcgca tggtcgagga
     4321 aggccttgaa cttgatctcg gcgttgcaca gcacatcggg attcggcgtg cggccggcct
     4381 ggtactcgcg caggaagtcc gcgaacaccc ggtcccggta gtcggccgcg aagttgacgt
     4441 gctcgatctc gatgcccagc acgtcggcga ccgaggcagc gtcgaggaag tcctggcgtg
     4501 acgaacagtg ctcgctgtca tcgtcatcct cccagttctt catgaagatg ccgatcactt
     4561 cgtgcccctg ctgcttgagc aggtgcgcac tgactgccga atcgacgccg ccgctcagcc
     4621 cgacgacgat gcgcttgcgc acggtcatgt cttgatcgcc gggctgtaca cgctcgcctc
     4681 ggtggagatc agctccagcg gatgccgccg cccggccaca tggtcctcga tgctctgcag
     4741 caccatgctg ctgcggtggc gcggggtgca ctcgcgcaat tcctccagcg tcatccagac
     4801 cgtgcgcacg atgccgtcat cgagggcccg gccgggcaag gggtccccga cggtaccggc
     4861 ataggcaatg cggaagtagg tgacatcctc gccccgcgac ggtcgctgga aacgaccgag
     4921 gtagaccccg acgatcgact gcggcgtgaa aacgcaggcg gtctcctcca gcacctcgcg
     4981 gaccacgcct tcctgcgggg attcggcctg atcgaggtgg ccggcggggt tgttgaggcg
     5041 caggccttcc ggcgtgtgct cctcgaccag caggaatcgg ccgtcgcgct cgatcaccgc
     5101 cgccaccgtc acattaggct tccatcgttc attcatggag cacgatggta gcggccgaag
     5161 ctttttcggg cgccggcccg ggggcgacaa gatcggctca accctaggcg ctctccggct
     5221 cgagttgtgt aagctgacat cggttaaaac gctcaccctc gcgcccccgc gcgcatccca
     5281 cgagccggag gaggcttcat gctgataggg atcccgctcg agacagcgtc cggcgaaaca
     5341 cgcgtggccg tgacgcctga aaccgccagg aagctgacag gccagggcca cacggtccgc
     5401 atccagtccg gtgccggcgt ggccgccagc gccaccgacg aggcctactc cgccgccggt
     5461 gccgagatca ccgacgcagc cggcgcgttc ggctgcgagc tggtactgaa ggtccgcgca
     5521 ccgagcgcgg ccgaactgcc gatgctgaag gcgggctcgg cactggtggg catgctgaat
     5581 ccgttcgatg gcgagggtct gcagcgcctc gccacggccg gagtgaccgg tttcgcgctg
     5641 gaggctgcgc cgcgcaccac gcgtgcgcag agcatggacg tcctcagctc gcaggccaac
     5701 atcgccggct acaaggccgt gatgatcgcc gccgacaagt accagcgctt cttcccgatg
     5761 ctgatgacgg cggccggcac ggtgaaggct gcgcgggtgg tgatcctggg cgtcggtgtc
     5821 gccggcttgc aggccattgc gaccgccaag agactgggcg cggtgatcga ggccagcgac
     5881 gtgcggccga gcgtcaagga gcaggtcgag tcgctgggcg ccaagttcat cgaggtctcg
     5941 tacgacaccc ctgaagagaa ggaagcggcg gtgggcgtcg gcggttacgc gaagccgatg
     6001 ccgcagagct ggcttgaccg gcagaagatt gaggtcgcca agcgggtggc ggcggccgac
     6061 gtcgtcatct ccaccgcgtt gatcccgggc cgcgcggcac cgacgctggt gaccgaggag
     6121 atggtgaagg cgatgaagcc gggctcggtg atcgtcgaca tcgccgccgg caaggggccg
     6181 aacggcgtcg gcggcaactg cccactgtcc gaggcggaca agaccgtcgt caagcacggc
     6241 gtgaccctcg tcggcgagac caacttgccg gcactcgtcg cggctgactc gtcggccctg
     6301 tatgcacgca acgtgctcga cttcctcaag ctggtgatca ccaaggaagg cactttcaac
     6361 gtgccgatgg acgacgacat cgtggcggcg tgcctgatga cgcagggcgg cgaagtgaag
     6421 aggacgtcat gagaagcagt cgcccaagga atccttgccg atcggcgtgt tgctgccgga
     6481 acactgcgac ctggagtcgg acatgaaccg ggtgatgttg cgcgcgaagt tgcaccgggc
     6541 gaccgtgacg gaggccgacc tgcattacga agggtcgtgc ggcatcgaca gcagcctgct
     6601 cgaacaggcc gacatgcgcg agttcgagcg catcgagctc tacaacgtga acaacgggga
     6661 gcgcttctcg acgtacgcca tcaaggcggc gcccggatcg ggcgtcatct cgctcaacgg
     6721 tgcggcggca cgccgggcac aggtcggaga cctgctgatc atctgcacct acgcgacgat
     6781 gaccgaggcg gaggcggcgg cctacaagcc gaaggtgctg ttgctcgacg agtgcaaccg
     6841 cgtcaaggaa gttcgcaagt tctgacaccg accaggaaac gacgcatgga catcatctcg
     6901 cccacgatca ccaacctgat catcttcgtg ctcgcgatct acgtcgggta ccacgtcgtc
     6961 tggaccgtga cgcccgcgct gcacaccccg ctgatggcgg tcaccaacgc gatctcggcg
     7021 atcgtgatcg tcggcgcgat gctggcggcc gcactgaccg agaccacgct gggccagacc
     7081 atgggggtgc tggcggtggc actggccgca gtcaacgtgt tcggcggctt tctggtgacc
     7141 cgccgaatgc tcgagatgtt caagaagaaa gagaagaaag ccgtcgcgcc ggagacagcc
     7201 aaatgagcct gaacctcgtc acgctgctct atctggttgc gagcatctgc ttcatccagg
     7261 cgctgaaggg cctgagccac ccgaccacct cgatccgcgg caacctgttc ggcatggtcg
     7321 gcatgacgat cgccgtgctg accaccgcgg ccctgatcgt caagctggcc ggcgcctcga
     7381 cggcttcggc ttccaccgga gggctgctct acgtcatcgg tgcgctggtg gtcggcggcg
     7441 ccgtcggcgc cgtgatggcc aagcgcgtcg agatgaccaa gatgcccgag ctggtggcct
     7501 tcatgcacag catgatcggt ctggcggccg tgttcatcgc ggtcgccgcg gtggccgagc
     7561 cgtacgcttt caacatcgtc gccaagggcg aaccgattcc taccggcaac cgtctcgagc
     7621 tgttcctggg cgcggcaatc ggcgcgatca ccttcagcgg ctcggtcatc gccttcggca
     7681 agctcagcgg caagtacaag ttccggctgt tccagggcgc tccggtgcag ttcgccggcc
     7741 agcacaagct gaacctgatc ctcggactga ccaccgtcgg tctggggctg ctgttcatgt
     7801 tcactggcag ctggacggca tttttcgcga tgctggcgct ggccttcgtg atgggcgtgc
     7861 tgatcatcat tccgatcggc ggcgcagaca tgccggtggt cgtctcgatg ctcaacagct
     7921 attcgggctg ggccgccgcc ggcatcggtt tcagcctcaa caactcgatg ctgatcatcg
     7981 cgggcagcct ggtcggctcg tcgggcgcga tcctcagcta catcatgtgc aaggcaatga
     8041 accggtcgtt cttcaacgtg atcggcggcg gcttcggcgg agaagcgaca tcggccggtg
     8101 gcgcgaaggc agaggcccgg ccggtcaaga gcggcagcgc cgacgacgcc gccttcgtgc
     8161 tgggcaatgc cgagacggtg gtgatcgtgc c
//
LOCUS       DAOORV010000145         8131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10910_length_8131_cov_5.780089, whole genome shotgun sequence.
ACCESSION   DAOORV010000145 DAOORV010000000
VERSION     DAOORV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10910_length_8131_cov_5.780089"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKV56_15065"
     CDS             <1..824
                     /locus_tag="PKV56_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HOX69179.1"
                     /translation="ADPDVRTADPLLAGGNTVVTAAGVVGAGNVVVNGAANTPASVGI
                     VPASMFDFPDLSTPYVIRPALTAAEPIDQAAALTGSLTVTSVKNEYLTNPGITAGTNW
                     VFSSPTRRYTVAVDYSTTPFRRVFSAYGSSAEQYFYDTNTLFDSASGQVCVLNVTPTS
                     YDQEETTPTSGVGFVISPGGQAAPLTFCGEVSVLSFNNASGTSVLGALLARKDIDLNY
                     TDGWVSIATPGNPNGGAPIGLPILGASFETAFNPAASAGIAGNYGLTFGHRFTRP"
     gene            984..1865
                     /locus_tag="PKV56_15070"
     CDS             984..1865
                     /locus_tag="PKV56_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX69180.1"
                     /translation="MLNSVAQTIRCSFLCAALVCVSALAEEAPLVSGAKVSITAADIQ
                     AEAQQVPAAARKEVLAKPDKVMLLASNLYVRRALAAEAAEQGLAVDPIVAAALKIAQD
                     KVLSDALLVKIDQANTPTDAALEQFTLARYRTESQRFQKPEETRARHILLKKGSAEAQ
                     AEAEKVLAELKAGANFEELAKQYSQDPGSAARGGDLGFFRRGQMVAEFDTALAALVKP
                     GELSGIVESQFGLHIIRLEERKEARPVSYEEIHTTLKQETLAKLLNDGRSREAQRLLG
                     AAKFDHSAAATISANQR"
     gene            1928..2728
                     /locus_tag="PKV56_15075"
     CDS             1928..2728
                     /locus_tag="PKV56_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX69181.1"
                     /translation="MRMLLDEVRALWAARGLVWVLAKREISARHAGSVAGAVWAYLPP
                     LLTVAAYYLVFDVVFAMRLGEQAPVRSVGAYLIVGSLPWMAFCDSISRATNSLIDAGS
                     LLQKNPLPPALFPARTVVASAVIFGPLIALMTLAYAPMHRFSPALVALLPLALLQVVL
                     CWLLGYLLAVCAAALRDVTAIVGFVLSVGIYLSPVLFPASMFPAAWAWVLWLNPMAPL
                     VGGYQSVVLSGQWPQWSTWIVTLAWITLTALLLDKVVKRSQEQLVDWL"
     gene            2725..3978
                     /locus_tag="PKV56_15080"
     CDS             2725..3978
                     /locus_tag="PKV56_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX69182.1"
                     /translation="MSELAESDGETSAVLRLDGVGKEYRLYDSPRDRLSALITGHSKH
                     RSHWALRDVGLELHRGQCMGVVGDNGAGKSSLLKLIAGTLQPTTGRLTRHGRVTAILE
                     LGAGFHPDFSGRQNLYFGGSLIGISHEEMTKLERDILDFSELGDAIDRPVKTYSSGMT
                     VRLAFALVTAVEPDLLIIDEALAVGDQNFQKKCVQRIDAFRRNGCTILFCSHSLYHVR
                     HLCNVALWLDGGRVKALGDTESVLSSYEVHVRSQDTSSPPEQVIAVEQEGNTLEVPAG
                     KLPAARAPVTAKLVCVEVANLGEGTPRLLESTDLVVTVKAQLGTGEQPNFIIMLEQSR
                     GVGITSIATHAEGIAPHHLGDGVWQTTLTFPELPLHSGDYVVSAYLLDAHGLVVYEEW
                     MRCVEFKLVAPSLTPGIVRLPHRWS"
     gene            3975..4877
                     /locus_tag="PKV56_15085"
     CDS             3975..4877
                     /locus_tag="PKV56_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69183.1"
                     /translation="MKRLRGFLARLKRESLDLVELVLIPSLAAPLPWPLCFRIFRWVA
                     FHWRFLYREPCARALSQANQRGWVDEPTTWLAMRRLVTLVDHADFYLERTRSDRWLQW
                     HVDTDGHWTPPGKAAVWLTFHWGAGLWALRHAAASGLTASFMVASSHPDHFKGRTVLR
                     HYVTRRNASVTRLLGRPTIDVSAPLRPALKVLQAHEQLVAVVDVPSDQVAASQQIQLL
                     GMPARVPRALFRLAVERSIPVGVFLPGICLSTGRRLLRLRDFGTPTDVDALVNEVFAE
                     LERAITECPPAWHFWSEADRFFRA"
     gene            complement(4930..6027)
                     /locus_tag="PKV56_15090"
     CDS             complement(4930..6027)
                     /locus_tag="PKV56_15090"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX69184.1"
                     /translation="MRSTSGEHYVALDHVRAVAVMLVFSWHFMHGPRGYPVPFDGTPL
                     WGPLVLFDEGHVGVALFMTLSGYLFAKLLDGRQVNYPLFFWNRLLRLMPLLLLVLLIH
                     AVIEAVRAHSAEAAFSYLLSMVPGFVYPKWPNGGWSIAVELQFYLLLPLILLLLRARP
                     RWLIGLVLVAAVAWRAAYHSMTGEVHSLAYFTIFGRIDQFLLGILAFHWRHSFSGLRY
                     WAAVAVVSLIQLYWWFDRSGGFFLQPSYPSPAPLWIVLPTLEGLAFSVLIVWYDTSFA
                     FKPGRVSNLFSTLGEYSYSIYLLHYFFVFELAAWIHQRVVDISSIYVAFPVSMVALIL
                     VLPVGYLSHRFIERPFLKLRRPYIRPAVEPV"
     gene            complement(6073..6669)
                     /locus_tag="PKV56_15095"
     CDS             complement(6073..6669)
                     /locus_tag="PKV56_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX69185.1"
                     /translation="MHSSSLQHVRQLVDRHLTDQPTLNVIDIGSYDVNGSYKPMFDRD
                     GWTYTGVDLTAGPNVDVVLSSPYRLPFASYSVDVIVSGQAFEHVEFFWLTWLEMARVL
                     KPGGVIFLLAPSRGPEHRYPQDCWRFYPDGYRALAAYGDLEMVSVTTDWAEHAEPDSA
                     PWGDTVGVFRQRPMSWAKRLRRAAMQRLRYLLLPGYRA"
     gene            complement(6756..>8131)
                     /locus_tag="PKV56_15100"
     CDS             complement(6756..>8131)
                     /locus_tag="PKV56_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HOX69186.1"
                     /translation="PVVLLVMHDREGGTLRHMQELAAWAVRDAVFFVLRPASGPSVQL
                     DLLGASEGFRLSFQLPSEFTPLVDALRGLGVGHVHFHHLLGHTPDVLALPAHLGVRYD
                     FTAHDYHAFCPQISLTGSDNSYCGEEGPDQCRRCLSRSPAPGGVSIEQWRVTHGSLIA
                     GARHVLVPSHDAAQRFLRYAPSADVRYAPHDELLEPVPHPVPVPRQLATGAPLKVVVI
                     GALSPIKGADLLEDVATLAAQTSARIDFHLLGFAYRNLRTRPRASLTVHGQYEDADLA
                     RLLDWLQPDLVWFPALWPETYSYTLSACLEQGLPVVAPDLGAFAERLSGRAWSWIRPW
                     KAAPSDWLAFFNEVRERHFATGQSPAPTHRLQSLAQDPRVRPWSYARDYLSAVPAPGH
                     PAALSPEFLARFGSNRAHGVSALRQGFKQSLLMAILRLRAMPMLRSVARAIPLRWQTR
                     VKSWLRA"
BASE COUNT         1533 a         2641 c         2532 g         1425 t
ORIGIN      
        1 ttgccgaccc tgacgtccgc acggctgatc cattgctggc tggtggtaac acggtagtta
       61 ccgcagctgg tgttgttggc gctggtaacg tggtcgtcaa cggcgctgcg aacaccccgg
      121 cttccgtggg tatcgtgccg gcgtcaatgt ttgacttccc agacctgtcc acgccgtacg
      181 ttatccgtcc ggctctgacc gccgctgagc ctatcgatca ggccgctgcc ttgaccggtt
      241 cgctgaccgt cacctcggtc aagaacgagt acctgaccaa ccctggcatc actgccggta
      301 ccaactgggt cttctcgtcg cctacccgtc gttacacggt ggctgtggac tactcgacca
      361 cgcctttccg ccgcgtcttc agcgcctacg gcagctcggc tgagcagtac ttctacgaca
      421 ccaacacgct gttcgacagc gcatctggcc aagtctgcgt gctgaacgtg acgcctacgt
      481 cgtatgacca ggaagaaacg accccgacca gcggtgttgg tttcgtcatc tccccagggg
      541 gccaagccgc tcctctgacg ttctgcggcg aagtcagtgt cttgagcttc aacaacgctt
      601 cgggtacctc ggttctcggc gctttgctgg cccgcaagga catcgacctg aactacacgg
      661 acggctgggt cagcatcgcc accccaggca acccgaacgg tggcgctcct atcggcctgc
      721 caatcctggg tgcttcgttt gaaaccgcct tcaacccagc tgcttcggct ggcatcgctg
      781 gcaactacgg cctgaccttc ggacaccgct tcacccgtcc gtaagttgag ctgctagagt
      841 tctactgttg ttgaaaaagg gcggggtttc cccgcccttt tttcattgga gcgaaccaat
      901 cactcgttcg ccggaaccaa ctgcgtcaac gccaattgct cgagatcggg cttgcgcttc
      961 cacctcttgc ttttccattt cccatgctga attcagtcgc ccaaaccatc cgttgctcgt
     1021 tcctctgtgc cgctctcgtc tgcgtatcag ccttggcaga agaagcgccc ctggtatccg
     1081 gagccaaagt cagcatcacc gctgccgaca tccaggcgga agcccagcag gttccggccg
     1141 ctgcgcgcaa ggaagtgctg gcgaaaccag acaaggtgat gctgctggct tccaacctgt
     1201 acgtgcggcg tgcgctcgcg gcagaggccg ccgagcaagg cctggccgtc gacccgatcg
     1261 ttgcagcagc gctgaaaatt gcacaggaca aggtcctgtc agacgccctg ctggtcaaga
     1321 ttgatcaggc caatactccg accgatgcgg cgcttgaaca attcaccctc gccagatacc
     1381 gaacggaatc ccagagattc cagaagccgg aagaaacccg tgcccggcat atattgctga
     1441 agaagggatc tgcggaagcg caggcagagg cagaaaaggt tctggccgaa ttgaaggcag
     1501 gcgccaattt cgaggaactg gccaagcagt attcgcaaga cccgggaagc gcggccaggg
     1561 gcggcgatct gggcttcttt cgccgtgggc agatggtggc agagttcgac acggcgcttg
     1621 ccgccctggt caaacccggc gaactgagcg gcatcgtgga aagccagttc ggcctgcaca
     1681 tcattcgcct cgaggagcgc aaggaggctc gacccgtgag ctatgaagaa atacacacca
     1741 ccctgaaaca ggaaacgctc gcgaagctgt tgaacgacgg tcgatcgcgc gaagcacaac
     1801 gcttgttggg tgcagccaag ttcgatcatt ctgcggctgc aacgatttcc gccaaccagc
     1861 gctgacccac gctgccacgg acccctaccc cgcgtatgac ttcgcccgcg cgtggcgcac
     1921 aggttgactg cggatgcttc tggatgaagt gagagccctc tgggcggcac gcggcctggt
     1981 atgggttctg gcaaagcgtg agatttctgc ccggcacgcc ggctccgtgg cgggcgcggt
     2041 gtgggcgtac ctgccgcccc tgctcacggt cgccgcgtac tacctggtgt tcgatgtcgt
     2101 cttcgcgatg cgactgggtg aacaggcgcc ggtccgttcg gtcggggcct acctgatcgt
     2161 cggatcgctt ccgtggatgg cgttctgcga ctcgatctct cgtgcgacga acagcctcat
     2221 cgatgcaggc agcctgctgc agaagaaccc cctgcctccg gcgctgttcc cggccagaac
     2281 tgtcgtcgcc agcgccgtca tcttcggacc gctgatcgcc ctgatgacgc tggcatacgc
     2341 cccgatgcac cgcttttctc ccgccctggt ggccctgctg cctctggccc tgctgcaggt
     2401 ggtgctgtgc tggctgctgg gctatctgct ggctgtctgc gccgcagctc tgcgcgacgt
     2461 gacagcgatc gtcggcttcg ttctgtcggt cggcatctac ctgtcgccgg tgctcttccc
     2521 ggccagcatg tttccagccg cctgggcatg ggtgctatgg ctgaatccga tggcgccact
     2581 cgtcggaggc taccaatcgg tggtcttgtc cggacaatgg ccgcaatgga gcacgtggat
     2641 cgtcacactg gcctggatca cgctgaccgc gctgctgctc gacaaggtag tcaagcgcag
     2701 tcaggagcaa ctggtggatt ggctgtgagc gaactcgcgg agtctgacgg ggagaccagc
     2761 gctgtactgc ggctggatgg ggtgggcaag gagtaccgtc tctatgactc gccccgagac
     2821 aggctgtctg cgctcatcac cggtcattcg aagcacagga gccactgggc gcttcgcgat
     2881 gtcggcctgg aattgcaccg cgggcaatgc atgggcgtcg tgggtgacaa cggcgccggc
     2941 aagagttcct tgctgaagct gattgccggc accttgcagc cgaccaccgg acggctgacg
     3001 cgccacggac gtgtcaccgc gatcctcgaa cttggtgccg gattccaccc ggacttcagc
     3061 gggcggcaga acctgtactt cggtggcagc ctgatcggca tctcgcacga ggagatgacg
     3121 aagctggagc gcgacatcct cgacttctcc gagctcggtg acgcgatcga tcgcccggtc
     3181 aagacctatt cctcggggat gacggtgcga ctggcttttg cgctcgtcac cgcggtcgag
     3241 ccggacctgc tcatcatcga tgaagcgctg gcggtgggtg accagaactt tcagaagaag
     3301 tgcgttcagc gcatcgatgc cttccgccgc aacggttgca cgattctttt ctgctcgcac
     3361 agcctctacc acgtgagaca cctctgcaac gtggctctgt ggctcgacgg cggtcgcgta
     3421 aaggccctgg gcgacaccga gtcggtcctg tcgtcgtacg aagtacatgt ccgatcgcag
     3481 gacacctcct cgccgccaga gcaagtcatc gccgtcgaac aggagggcaa cacgctcgag
     3541 gtccctgctg gcaagcttcc cgcggcacgc gctcccgtga cggccaaact tgtctgcgtc
     3601 gaagtcgcga acctcggaga gggaacgccg cggctgctgg agtcgaccga tctggtggtc
     3661 acggtcaagg cgcagttggg aacgggcgag cagccgaact tcatcatcat gctggagcag
     3721 tcccgcgggg tcggcatcac ttcgatcgcc acgcatgcgg agggcattgc gccccaccat
     3781 ctgggcgacg gcgtctggca gaccacgctg acctttcccg aacttccgct gcactcgggc
     3841 gactatgtcg tcagtgcgta cctgctcgat gcgcacggtc tggtcgtgta cgaggaatgg
     3901 atgcgatgcg tcgaattcaa gctcgtggca cccagcctga caccgggcat cgtccgcctg
     3961 ccgcaccgct ggtcgtgaaa cgcctgagag gattcctggc ccggctcaag cgcgagtcgc
     4021 tcgatctggt cgaactggtg ctgattccca gccttgcagc accccttccc tggccgctgt
     4081 gcttcaggat atttcgctgg gtggcatttc actggcgctt tctgtatcgc gagccttgcg
     4141 cacgcgcgct gtctcaggcg aatcagcggg gatgggtcga cgagcccacc acctggctgg
     4201 cgatgcgccg gctggtcaca ctggtcgacc atgcggactt ctatctggaa cgcacgcgct
     4261 cggaccgatg gcttcaatgg catgtggata ccgacggcca ctggacgccg ccgggcaagg
     4321 ccgcggtgtg gctcaccttc cactggggcg cgggcctctg ggcgctgagg cacgccgccg
     4381 cctccggtct caccgcaagc ttcatggtgg cctcctccca ccccgatcac ttcaaggggc
     4441 gcaccgtact tcggcactac gtcacccgcc gcaatgccag cgtgaccagg ctgctggggc
     4501 gaccgacgat agacgtgagc gctccgctgc gaccggcgct caaggtattg caagcccacg
     4561 aacaactggt tgccgtcgtg gacgtgccat ccgaccaggt ggcagcgagc cagcagatac
     4621 aactgctggg gatgccggcc cgggtgccgc gcgccctgtt tcgcctcgcc gtggagcgct
     4681 cgattccggt cggcgtgttc ctgccgggca tctgcctgtc gaccggacga cgcctcctgc
     4741 gccttcgaga cttcggcacg cccacggacg tcgatgccct ggtgaatgag gtcttcgcgg
     4801 aactcgaaag ggccatcacc gaatgcccgc ccgcatggca cttctggagc gaggctgacc
     4861 gtttttttcg agcctgacac tgccctagaa gggatcgaac tcgttgtcgg tcaaatcagc
     4921 aggcaggctt cacactggct cgaccgccgg gcggatgtac ggcctgcgca gcttcaggaa
     4981 cggtcgttcg atgaatcgat gactcaggta ccccacggga agcaccagga tcagcgccac
     5041 catcgagacc gggaacgcga catagatact ggagatatcg accacgcgct ggtgaatcca
     5101 ggccgccagt tcgaacacga agaagtagtg cagcaggtag atggaatacg aatattcgcc
     5161 cagggtcgaa aacaggttcg atactcgccc cggcttgaac gcgaacgacg tgtcatacca
     5221 gacgatcagt accgagaatg ccagtccttc aagcgtcggc aacacgatcc acagcggcgc
     5281 cggtgaggga tagctcggct gcaggaagaa gcctccactg cggtcgaacc accagtaaag
     5341 ctggatcagc gagacgaccg caaccgccgc ccagtagcgc aaccctgaga agctgtgtct
     5401 ccagtgaaag gccagtatcc ccagcaggaa ttgatctatc cgcccgaaga tcgtgaagta
     5461 ggccaggctg tgcacctcgc ccgtcatgga gtgataggcg gccctccagg ccaccgccgc
     5521 caccaggacc agaccgatca gccagcgtgg ccgcgcacgc aggagcagca agatcagcgg
     5581 aagcagcagg tagaactgca gctcgaccgc gatggaccag ccgccgttag gccacttcgg
     5641 gtacacgaaa cccggcacca tggacagcag gtaggaaaac gctgcctctg cgctgtgcgc
     5701 gcgaaccgcc tcgatcaccg catggatcag caggaccagc agcagcagcg gcatcagacg
     5761 cagcaggcga ttccagaaga acagcggata gttcacctgc ctgccgtcga gcagcttcgc
     5821 gaagagatag ccgctcaggg tcatgaaaag tgccaccccg acatgccctt cgtcgaacag
     5881 gaccagcggg ccccacaagg gcgttccgtc gaacggaacc ggatagccgc gcggcccgtg
     5941 catgaaatgc cacgagaaga ccagcatcac ggccacggcc cggacgtggt ccagggccac
     6001 gtaatgctcg ccggacgtgg atctcatcgg atgagagtca gggcggccat cgtgaacatc
     6061 accgaagcag gttcaggccc ggtatcccgg cagcagcaga taccgcagcc gctgcatcgc
     6121 agcacgccga agccgcttcg cccacgacat cggccgctgc ctgaacactc ccacggtgtc
     6181 gccccagggc gcactgtccg gctcggcatg ctcggcccaa tcggtcgtga ccgacaccat
     6241 ctccagatcg ccgtacgcgg ccagggctcg gtacccgtcc gggtagaagc gccagcagtc
     6301 ctgcggatac cggtgctccg gcccgcgcga cggtgccagc aaaaaaatga cgccgccggg
     6361 cttcagcaca cgcgccatct ccagccaggt cagccagaag aactccacat gctcgaaggc
     6421 ctgcccggaa acgatcacat cgaccgagta gctcgcgaac ggcaatcgat acggcgacga
     6481 cagcaccacg tcgacattgg gcccagccgt cagatcgaca ccggtgtacg tccatccgtc
     6541 gcgatcgaac atcggcttgt agctgccgtt gacgtcgtag cttccgatgt cgatgacgtt
     6601 cagggtcggc tggtcggtca ggtgacgatc gaccagttgt ctgacgtgct gcagggaact
     6661 cgagtgcatg cggatgccgt gaccgggtta attggaatgg gtaatgtcgc cgctcggcgc
     6721 cagcgcacga aagagtccgc agaccgaaga gaacatcagg cccgaagcca gctcttgact
     6781 cgcgtctgcc agcgcagggg aatggcacgc gccacgctgc gcagcatcgg catcgcgcgc
     6841 aatcgcagta tggccatcag caacgactgc ttgaagccct gtcgcaatgc actcaccccg
     6901 tgcgcccggt tgcttccgaa tctcgcaaga aattccgggc tcagcgctgc aggatgaccc
     6961 ggcgccggaa cggcgctcag gtagtcccgc gcatacgacc acggtcgcac tcgcggatcc
     7021 tgcgcaagcg actgcaggcg atgcgtggga gccgggctct ggccggtggc gaaatggcgc
     7081 tcacgcactt cattgaagaa cgcgagccaa tctgacggag ccgccttcca gggccggatc
     7141 cagctccacg cacgaccgct gagccgctcg gcgaaggcgc ccaggtccgg tgccaccacg
     7201 ggcaatccct gctcgaggca cgcgctgagg gtgtagctgt aggtctcggg ccacagggcg
     7261 ggaaaccaga ccagatcggg ctgaagccag tcgagcaggc gcgccagatc ggcgtcttcg
     7321 tactggccgt gaaccgtgag actggcgcga ggccgggtgc gcaggttccg gtaggcaaac
     7381 ccgagcagat ggaagtcgat gcgagccgac gtttgagctg ccaaggtcgc gacatcctcg
     7441 agcaggtcgg cgcccttgat cgggctgagc gctccgatca cgacgacctt cagcggagcc
     7501 cccgtggcga gctggcgtgg caccggcacc gggtgcggca cgggctccag cagttcatcg
     7561 tgcggcgcat accgcacgtc ggcggaaggg gcgtaccgca ggaagcgctg ggccgcgtcg
     7621 tggctgggca ccagcacatg ccgcgcaccg gcgatcaggc tgccgtgggt gacacgccac
     7681 tgctcgatgg acacgccgcc cggcgccggc gagcggctca ggcagcgccg gcactgatcc
     7741 ggcccttcct cgccgcagta agagttgtcg ctgcccgtga gactgatctg cgggcagaac
     7801 gcgtggtagt cgtgcgcagt gaagtcatac cttaccccca gatgcgccgg cagcgccagc
     7861 acatcgggag tgtgtccgag caggtggtga aagtgcacat ggccgacgcc cagcccgcgc
     7921 agtgcatcca ccagtggcgt gaactcggag ggcagctgga aggacagccg gaagccttcg
     7981 cttgcaccga gcagatcgag ttgcaccgag ggtccgctgg ccggcctgag cacgaagaac
     8041 accgcatccc ggaccgccca tgcggccagc tcctgcatgt gtcgaagcgt gccgccttca
     8101 cgatcgtgca tcaccagcag gacgacgggc t
//
LOCUS       DAOORV010000146         8091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_10997_length_8091_cov_7.289945, whole genome shotgun sequence.
ACCESSION   DAOORV010000146 DAOORV010000000
VERSION     DAOORV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10997_length_8091_cov_7.289945"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="glgA"
                     /locus_tag="PKV56_15105"
     CDS             complement(<1..1385)
                     /gene="glgA"
                     /locus_tag="PKV56_15105"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOX69187.1"
                     /translation="MRILHAAAEVFPLVKTGGLADVVGALPQALIDQGADVRLVLPGL
                     PAIVDAVLHQKAVCSLGPLFGAAHVTLRLGQLPYSRVPVYVIDAPYLYRRGGSPYQAS
                     TGVEWSDNLQRFGLLGWVAAHLAAGELDPNWVPDVLHAHDWHAAMSCAYVAAHPPTTA
                     ATVFTVHNLAYQGLFAPGDFPLLGLPSRFMAPQALEFHSQLSFMKAGLKFAHRVTTVS
                     PTYAIEMATHEFGCGLEGVIRGRGADVSGILNGVDGAVWNPATDTGIIARYTAECAEA
                     DCVAKARCKSALQAEMGLLVEPRAPLFAVVSRLTPQKGLDLVLAALPALLQGGGQLAL
                     QGAGDPDLEAAFGALAAAHPGRVAVRIGYDEGFAHRIIAGADAILVPSRFEPCGLTQL
                     YGLRYGTVPVVRRVGGLADTVIDASDHARTLGSARATGFVFDHSTASALAEAIERVLA
                     VYAEPAQWIEL"
     gene            complement(1446..2726)
                     /gene="glgC"
                     /locus_tag="PKV56_15110"
     CDS             complement(1446..2726)
                     /gene="glgC"
                     /locus_tag="PKV56_15110"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOX69188.1"
                     /translation="MPNDHTNNLALSLELPKRAVALVLAGGRGSRLKNLTDRRAKPAV
                     YFGGKFRIIDFALSNCMNSGIRRIGVITQYKSHSLLQHLQRGWAFLKSEMNEFVDLLP
                     AQQRTDDESWYRGTADAVYQNQDIIASYRADYIVVLAGDHIYKMNYALMLADHVNKGR
                     DCTVACITVPREEASAFGVMSVGANSEVIDFVEKPVDPPAMPGHPDKALASMGIYIFN
                     ARYLYKQLERDMADPDSSHDFGKDIIPHAVRQGSVSAHPFEQSCVNTRTGEEAYWRDV
                     GTIDAYWDANIDLTATDPLLNLYDTRWPIWTHQGQLPPAKFVHNQDDRRGMAIESLVS
                     GGCIVSGLVFRTLLFSSVRVHSHAVVNWSVLLPGVQVGRGARITRAVIDRDCVIPDGM
                     VIGEDDALDAERFHRSEKGITLVTREMLKKLANV"
     gene            3040..5451
                     /locus_tag="PKV56_15115"
     CDS             3040..5451
                     /locus_tag="PKV56_15115"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOX69189.1"
                     /translation="MQHGIEDHLLKTVGVDANEATPVELMQAVSQVAREQLSKRWVAT
                     QGADRDSKARRVYYLSMEFLMGRTLGNALAALDLTGSAAQALANQARSLEDIEDREPD
                     AALGNGGLGRLAACFLDSMAELGLPSFGYGIRYEYGMFAQDIVEGCQVEYPDPWLADG
                     TPWEFPRASVSFPVYFGGWVEHVGLPGHEKAVWRHAAEVAAKAYDMVVPGHGTKRVST
                     LRLWKAVAPAHIDLHAFNTGDYARAAGVKNEFENISWVLYPNDSTPAGRELRLRQEYF
                     FVAASVQDLVGRHLDEHRTLDNLADKVAIHLNDTHPAIGVAELMRVLCDDHGMAWADA
                     WAICRKTFSYTNHTLMPEALETWPVGLMQHVLPRHLEIIFRINQEFLDEAARHRPGDL
                     GFLSRLSLIDEHGERRVRMAHLSIVGSHQVNGVSALHSDLLVQTIFADFASLWPDRFT
                     NMTNGVTPRRWLSQANPGLTALLDRTIGAGWRRDLNQLQLLKDKKADPAFRDEFMAVK
                     RANKARLAAHIERSTGVKVHPGSLFDVQVKRIHEYKRQLLNVLHVVTRYQAIVANPGA
                     DFVPRTVIFAGKAASSYHSAKSIIRLIHDVGAVINNDPRVGDKLKLVFIPNYGVSVAE
                     IIMPGADLSEQISTAGTEASGTGNMKLALNGALTIGTDDGANIEIRQNVGDDNIFIFG
                     LKTPEVQALKHSGYQPMRHYEGNTKLKAVLDAIASGHFSPDEPDRYRGLIDSLLWGGD
                     HYLLLADYDSYVATQAKVDDLYRQPTEWATRAIANVAGMGVFSSDRTIEQYARQIWHV
                     QPEIR"
     gene            5483..7690
                     /gene="glgB"
                     /locus_tag="PKV56_15120"
     CDS             5483..7690
                     /gene="glgB"
                     /locus_tag="PKV56_15120"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOX69190.1"
                     /translation="MPLLSAFDIERILSASHDDPFSVLGPHEMPSNGRADPRWCVRAF
                     LPGARKVGVTLPTRPDHVTALSCTDGEGLFEGVVGDLPRSAYRLQVTWQDGRDSVIDD
                     PYRFPPLLGDVDAWLFGEGSHLRPYEVLGATRHVLDGIEGTGFAVWAPNASRVSVVGD
                     FNMWDGRRHPMRLRRECGVWEIFLPGLAAGTRYKYEIRTRDGVLLPLKADPYALQSEL
                     RPATASVVARLPAVVPPSPARQSANALDAPISIYEVHLGSWRRVDEGGFQRWLSWQEL
                     ADTLVPYARDMGYTHLELMPIAEHPFDGSWGYQPIGLYSPTARFGDPQGFRRFVERCH
                     AEGLGLLLDWVPAHFPTDAHGLREFDGTHLYEYADPREGFHNDWNTLIYNFGRTEVGN
                     FLVGNALYWLERFGVDGLRVDAVASMLYRDYSRKPGEWIPNVHSGRENLEAIALLKRL
                     NEVVGIERSQAITLAEESTSFPGVSRPTYAGGLGFHFKWNMGWMHDTLLYMARDPIHR
                     KHHHDEMTFGMVYAYTENFVLPLSHDEVVHGKGSLIGKMPGDRWQKFANLRAYYGFMY
                     GHPGKKLLFMGGEFAQEGEWNHDRSLDWHLLGDRHHEGVQRLVRDLNYFYRETPALHQ
                     RDFDRGGFEWIEHNDAERSLLAFIRRGHDDATLVVVVCNFTPTVHTSYRLGVPRAGDY
                     HERINTDSSHYGGSNTGTPHGVGRSQPVAWQGQPHSVLLTVPPLATVMLEWRA"
     gene            7687..>8091
                     /locus_tag="PKV56_15125"
     CDS             7687..>8091
                     /locus_tag="PKV56_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69191.1"
                     /translation="MKPQQATSTGLTAGRPWPLGAHVDESGINLAVYSAHAQAIEWCV
                     FDPSGTKETARHPLVRSGDVWHAHLTGAGAGLVYGLRAHGPWCPQEGHRFNPHKLLLD
                     PRAREVVGDFQWSDLHFAFDRQRSAADERIGLA"
BASE COUNT         1430 a         2717 c         2644 g         1300 t
ORIGIN      
        1 atcagttcga tccactgtgc tggctcggca tagacggcca gcacccgctc gatcgcctcg
       61 gccagcgctg aggcggtgga gtgatcgaag acgaaaccgg tggcccgagc gctgcccagg
      121 gttcgggcat ggtcgctggc gtcgatgacc gtgtcagcca gacctccgac acgccgcacc
      181 accggaaccg tgccgtagcg cagcccgtac aactgggtca gcccgcacgg ttcgaaccgc
      241 gacggcacca ggatggcatc cgcccccgca atgatgcggt gcgcaaagcc ctcgtcgtag
      301 ccgatgcgca ccgccacgcg gcccgggtgg gctgcggcga gggcgccgaa ggcagcttcc
      361 agatcgggat caccggcgcc ctgcaaggcc agctgaccgc cgccctgcag cagcgcgggc
      421 agcgcggcga gcaccaggtc gaggcccttc tgcggcgtca atcgactgac caccgcgaac
      481 agcggtgcgc gtggctcgac gagcagcccc atctcggcct gcagggccga cttgcaacgg
      541 gccttggcca cgcagtcggc ctcggcgcat tcggcggtgt agcgggcgat gatcccggtg
      601 tcggtggccg gattccagac cgccccatca accccattca agatgcccga gacgtcagcg
      661 ccccggcccc gaatcacgcc ttcgaggccg caaccgaact cgtgtgtcgc catctcgatg
      721 gcgtaggtcg ggctgaccgt ggtcacgcgg tgcgcgaact tcaggccagc tttcatgaac
      781 gaaagctggc tgtggaattc gagcgcctgc ggcgccatga accgcgacgg caagcccagc
      841 agcgggaagt cgcccggcgc aaacagcccc tggtacgcga ggttgtgcac cgtgaacacc
      901 gtggctgcgg tggtcggcgg gtgggcggcc acataggcgc acgacatcgc cgcatgccag
      961 tcatgggcat gcagcacgtc gggtacccag ttcgggtcga gctctcctgc ggccaggtgg
     1021 gccgcaaccc agcccagcaa gccgaagcgc tgcaggttgt cggaccactc gacgccggtg
     1081 ctggcctggt acgggctgcc gccgcgccga tagagataag gcgcgtcgat cacgtacacc
     1141 ggcacgcgac tgtagggaag ctggcccaat cgcaaggtga cgtgggcggc tccgaacagc
     1201 ggaccgaggc tgcacaccgc cttctggtgc agcaccgcgt cgacgatggc cggcaggccc
     1261 ggcagcacca gccggacatc ggccccctga tcgatcaagg cctggggcag cgcaccgacc
     1321 acatcggcca gcccgccggt cttgaccagc ggaaagacct ccgccgcagc gtgcaggatt
     1381 ctcattggcg ggcgccgagc cagatcagag gatggcagca cggtgaagtg ggaatgaacg
     1441 acatctcaga cattggcgag cttcttcagc atctcccgag tgaccagcgt gatgcccttc
     1501 tcgctgcgat ggaagcgctc ggcatccagc gcgtcgtctt cgccaatgac catgccatcg
     1561 gggatcacgc agtcgcgatc gatcacggca cgggtgatcc gggccccacg gccgacctgc
     1621 acacccggca acagcacaga ccagttgacc accgcgtgcg aatgcactcg cacgctggag
     1681 aacagcagcg tgcggaacac cagccccgac acgatgcagc caccggagac cagcgattcg
     1741 attgccatgc cacgccggtc gtcctggttg tgcacgaact tagccggcgg cagctggccc
     1801 tggtgcgtcc agatcggcca gcgggtgtcg tagaggttga gcaacgggtc ggtcgcagtc
     1861 aggtcgatgt tggcatccca gtaggcatcg atggttccga cgtcgcgcca gtaggcctct
     1921 tcgccagtgc gggtgttgac acagctctgc tcgaacggat gtgccgatac gctgccctga
     1981 cgtaccgcat gcggaatgat gtccttgccg aaatcgtggc tcgaatccgg atcggccatg
     2041 tcgcgctcga gctgcttgta gagatagcgc gcattgaaga tgtagatgcc catgctggcc
     2101 agcgccttgt cgggatgacc cggcatcgca ggcggatcca cgggcttctc gacgaagtcg
     2161 atgacctcgc tgttggcccc gacggacatc acgccgaatg cgctggcctc ctcgcgaggc
     2221 acggtgatgc acgccacggt gcagtcgcgc cccttgttga catggtcggc cagcatcagc
     2281 gcgtagttca tcttgtagat gtggtcgccg gccagcacca cgatgtagtc ggcacggtag
     2341 ctggcgatga tgtcctggtt ctggtacacg gcatcggccg tgccgcggta ccagctctcg
     2401 tcgtccgttc gctgctgtgc aggcagcagg tcgacgaact cgttcatttc cgacttcagg
     2461 aacgcccagc cgcgctgcag gtgctgcagc aggctgtgcg acttgtactg cgtgatgacg
     2521 ccgatgcggc ggatgcccga attcatgcag ttcgacagcg caaagtcgat gatgcggaac
     2581 ttgccgccga aatacacggc gggcttggcg cggcgatcgg tcaggttctt caggcggctg
     2641 ccccgccctc ccgccagcac aagcgccacc gcccgcttgg gcagttcgag gctgagcgcc
     2701 agattgttgg tgtggtcatt gggcatcgtg tctcctgctg ctgcgtgatg tcgcccgagc
     2761 ccgcggtgac ctgcacgaga aaagtcgttg cttgtggatg ggattctttt ccagcatgga
     2821 gtgtgcctgt acccgcggcg cctgaaaacg tcgcgcgacg caccgcgcag ggcgggcccc
     2881 aaaaggcgcg aaacttgcaa agcctccggc acgccgcgct ggagagcgcc acggcaagca
     2941 ggcccgcggg ccgagtcagc tatccaaatc gaggaaggcc cggtgatgtc ccacacctcc
     3001 cccacccaac cgtccagcac cgcaccatcg gtggccgaca tgcagcacgg catcgaagac
     3061 cacctgctca agaccgtcgg ggtcgatgcc aacgaggcga ctccagtcga gctgatgcag
     3121 gccgtttcgc aggtcgcgcg cgagcaactg tcgaagcggt gggtcgccac gcaaggcgcc
     3181 gaccgcgaca gcaaggcgcg ccgcgtctac tacctgtcga tggaattcct gatgggtcgc
     3241 acgctgggca atgcgcttgc cgcgctcgac ctgaccggca gcgcggcgca ggcgctggcg
     3301 aatcaggccc gctcgctcga ggacatcgag gatcgcgagc ccgatgcggc gctcggcaac
     3361 ggcggcctgg gccgcctggc cgcctgtttc ctcgattcga tggccgaact cggcctgccg
     3421 tcgttcggct atggcattcg ctacgagtac ggcatgttcg cgcaggacat cgtcgagggg
     3481 tgccaggtcg agtaccccga cccgtggctg gccgacggaa cgccgtggga attcccacgc
     3541 gccagcgtca gctttccggt gtacttcggc ggctgggtcg agcacgtcgg cctgccgggt
     3601 cacgagaagg ccgtctggcg ccatgccgcc gaagtcgccg ccaaggccta cgacatggtc
     3661 gtgccggggc acggcacgaa gcgcgtcagc acgctgcggc tctggaaagc ggtggcaccg
     3721 gctcacatcg acctgcacgc cttcaacacc ggagactacg cgcgcgccgc aggcgtgaag
     3781 aacgagttcg agaacatctc gtgggtgctg taccccaacg acagcacgcc ggccggccgt
     3841 gagctgcggt tgcggcagga gtacttcttc gtggctgcgt cggtgcagga cctggtgggc
     3901 cgccacctcg acgagcaccg cacgctcgac aacctggccg acaaggtggc catccacctg
     3961 aacgacaccc atccggcgat cggcgtggcc gaactgatgc gcgtgctgtg cgacgaccac
     4021 ggcatggcat gggccgatgc ctgggccatc tgccggaaga cgttctcgta caccaaccac
     4081 acgctgatgc ccgaggcgct ggagacctgg ccggtcggtc tgatgcagca cgtgctgccg
     4141 cggcacctgg agatcatctt tcgcatcaac caggagttcc tggacgaggc ggccagacac
     4201 cggcctggtg acctgggctt cctgtcgcgg ctgtcgctga tcgacgagca cggcgagcgc
     4261 cgcgtgcgca tggcccacct gtcgatcgtc ggcagccacc aggtcaacgg ggtgtcggca
     4321 ctgcactccg acctgctggt gcagacgatc ttcgccgact tcgccagcct gtggcccgac
     4381 cgattcacca acatgaccaa cggcgtcacg ccgcgtcgtt ggctgtcgca ggcgaatccg
     4441 ggcctgaccg cgctcctcga ccgcaccatc ggcgctggct ggcgccgcga cctgaaccag
     4501 ctgcaactgc tgaaggacaa gaaggccgac ccggccttcc gcgacgaatt catggcggtc
     4561 aagcgagcca acaaggcccg actcgcggca cacatcgagc gcagcaccgg cgtcaaggtc
     4621 catccgggca gcctgttcga cgtgcaggtc aagcgcatcc acgagtacaa gcgccagctg
     4681 ctgaatgtgc tgcacgtggt cacacgctac caggcgatcg tcgccaatcc gggcgcagac
     4741 ttcgtgcccc gcaccgtgat cttcgcgggc aaggcggcat caagctatca ctctgccaag
     4801 tcgatcatcc gtctgatcca cgacgtgggc gcggtgatca acaacgaccc tcgtgtcggc
     4861 gacaagctga aactcgtctt catcccgaac tacggcgtat cggtggccga gatcatcatg
     4921 ccgggcgccg acctgtcgga gcagatctcg accgcaggca ccgaggcctc gggcaccggc
     4981 aacatgaagc tggctctcaa cggtgcgctg accatcggca ccgacgacgg cgccaacatc
     5041 gagatccggc agaacgtcgg cgacgacaac atcttcatct tcggactgaa gacacccgaa
     5101 gtgcaggcgc tgaagcacag cggctaccag ccgatgcggc actacgaagg caacacgaag
     5161 ctgaaggcgg tgctcgacgc gatcgcgtcc ggtcacttct cgcccgacga gcccgaccgt
     5221 taccggggcc tgatcgattc gctgctctgg ggtggtgacc actacctgct gctggccgat
     5281 tacgactcgt atgtggcgac ccaggcgaag gtcgacgatc tgtatcggca gccgaccgag
     5341 tgggcgaccc gagcgatcgc caacgtcgcc ggcatgggcg tcttctcgtc ggatcggacg
     5401 atcgagcagt acgctcgaca gatctggcac gtgcagccgg aaattcgctg acactcgccg
     5461 cagatcaccc cgacaccccg ccttgccact cctcagcgca ttcgacatag aacggatcct
     5521 cagcgcgagc cacgacgacc cgttctcggt gctggggccg catgagatgc cgtcgaacgg
     5581 ccgagccgac ccgcggtggt gcgtgagggc cttcctgccg ggtgcacgga aagtgggtgt
     5641 gacgttgccg acccgtccgg accatgtcac ggcactgtcg tgcaccgacg gtgagggcct
     5701 gttcgagggc gtggtcggcg acctgccgcg ttccgcctac cggctgcagg tcacctggca
     5761 ggatggtcgt gacagtgtca tcgacgatcc ctatcgcttt cctccgctgc tcggtgatgt
     5821 ggatgcgtgg ctgtttggcg agggctcgca cctgcgaccc tacgaagtgc tcggtgccac
     5881 acgacacgtt ctggacggca tcgagggcac cggtttcgcc gtgtgggcgc cgaacgcttc
     5941 acgggtcagc gtggtcggcg acttcaacat gtgggatgga cgccgtcacc cgatgcgact
     6001 gcggcgcgag tgtggcgtgt gggagatctt cctgcccggc ctcgcggcgg ggacgcgcta
     6061 caagtacgag atccgcacac gcgacggcgt gctgctgccg ctgaaggccg atccctacgc
     6121 cctgcaatcc gagttgcgtc cagcgaccgc cagcgtcgtg gcccgattgc ccgcggtggt
     6181 gccgccctcc cctgcacgcc agtccgcgaa cgcgctcgat gcaccgatca gcatctacga
     6241 ggtgcacctc gggtcctggc gacgcgtgga cgaaggaggc ttccagcgct ggctgagctg
     6301 gcaagaactc gccgacacgt tggtgccgta cgcacgtgac atgggctaca cgcacctcga
     6361 actgatgccc attgcagagc atccgttcga cggctcttgg ggctaccagc cgatcggcct
     6421 gtactcgccg accgcgcgct tcggcgatcc gcagggcttc cggcgcttcg tcgagcgctg
     6481 ccatgccgaa ggtctcggcc tgctgctcga ctgggtgccg gcgcactttc cgaccgatgc
     6541 gcacggactg cgcgaattcg acggcacgca cctgtacgag tacgccgatc cgcgcgaggg
     6601 cttccacaac gactggaaca cgctgatcta caacttcggc cgcaccgagg tcggcaactt
     6661 cctggtgggc aacgcgctct actggctgga gcgcttcggc gtcgacgggc tgcgcgtcga
     6721 tgcggttgca tcgatgctct accgcgacta cagccgcaag ccgggcgagt ggatacccaa
     6781 cgtgcacagc gggcgcgaga acctggaagc catcgccctc ctgaagcgac tcaacgaggt
     6841 ggtcggcatc gagcggtcgc aggccatcac gctggccgag gagtcgacct cgtttcccgg
     6901 cgtgtcgcgt ccgacctatg caggcggcct cggcttccac ttcaagtgga acatgggatg
     6961 gatgcacgac acgctgctct acatggcgcg cgacccgatc catcgcaagc accaccacga
     7021 cgaaatgacc ttcggcatgg tctacgcgta caccgagaac ttcgtgctgc cgctgtcgca
     7081 cgacgaggtg gtgcacggca agggatcgct gatcggcaag atgcccggcg accgctggca
     7141 gaagttcgcc aacctgcgcg cgtactacgg tttcatgtac gggcaccccg gcaagaagct
     7201 gttgttcatg ggcggcgagt tcgcgcagga aggcgagtgg aaccacgacc gcagcctcga
     7261 ctggcatctg ctcggtgacc gccatcacga gggagtgcag cgactggtcc gcgacttgaa
     7321 ttatttctac cgcgagaccc cggcgctcca ccagcgcgac ttcgatcgcg gcggcttcga
     7381 atggatagag cacaacgatg ccgagcgctc gctgctcgcc ttcatccggc gcggccatga
     7441 cgacgcgacg ctggtcgtcg tggtctgcaa cttcacgccg acagtgcaca ccagctaccg
     7501 gctgggcgtg ccacgtgccg gcgactatca cgagcggatc aacaccgatt cgagccacta
     7561 cggcggcagc aacaccggca ccccgcacgg cgtgggtcgt tcgcagccgg tggcctggca
     7621 gggacagccg cattcggtgc tgctgacggt gccgccgctg gccaccgtga tgctggagtg
     7681 gcgcgcttga agccgcagca agccacatca accgggctga cggccggacg cccgtggccg
     7741 ctgggagcgc acgtcgacga gagcggcatc aacctcgccg tgtactcggc gcatgcccag
     7801 gcgatcgagt ggtgcgtgtt cgacccgagc ggtacgaagg aaaccgcgcg ccaccccctg
     7861 gtgcgcagcg gcgatgtctg gcacgcccac ctgacgggtg caggggccgg tctggtctac
     7921 gggctgcgcg cgcacggccc ctggtgtccg caggaaggac accggttcaa tccacacaag
     7981 ctgctgctcg acccccgcgc aagagaggtg gtcggcgatt tccagtggag cgacctgcac
     8041 ttcgccttcg accgccagcg gtccgccgcc gatgagcgga tcggtcttgc c
//
LOCUS       DAOORV010000147         8028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11115_length_8028_cov_6.398470, whole genome shotgun sequence.
ACCESSION   DAOORV010000147 DAOORV010000000
VERSION     DAOORV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11115_length_8028_cov_6.398470"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKV56_15130"
     CDS             complement(<1..598)
                     /locus_tag="PKV56_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOX69192.1"
                     /translation="MTLPGSPQISYRGDTLMLEDCAVADLATRFGTPLYAYSRAAMLS
                     ALSAYQRALDGRDHLICYAVKANSNLAVLQTFAQAGCGFDIVSAGELARVLKAGATPS
                     KVVFSGVGKTRAEMRQALDAGVMCFNVESEAELEALSSVATQSGGSARISLRVNPDVD
                     ARTHPYISTGLKGNKFGIAHERAVAVYSRAASLPGISVV"
     gene            845..1204
                     /gene="cyaY"
                     /locus_tag="PKV56_15135"
     CDS             845..1204
                     /gene="cyaY"
                     /locus_tag="PKV56_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HOX69193.1"
                     /translation="MTTDSMITPLTDMEFRAKAGAVLSHIEVTLDRWLQQDVIDIDAS
                     RTGGLLEMSFPNGSKIVINTQPPLQELWLAARSGGYHFKHADGRWLDTRGGLEFYSAL
                     SACTSEQAGVGLSFEAA"
     gene            complement(1251..3509)
                     /locus_tag="PKV56_15140"
     CDS             complement(1251..3509)
                     /locus_tag="PKV56_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOX69194.1"
                     /translation="MAAGALSVLMLVALALAVAYPNLPEISGLTDYRPKLPMRIYAAD
                     GVLLGEFGEERRNFVSAADMPAVMKNAVLAIEDARFYQHGGVDYLGILRAGLANFGES
                     RSQGASTITMQVARNFYLSTEKTFTRKIYEILLALKIESLLTKDQILELYMNQIYLGQ
                     RAYGFASASEVYFGKPLKSVSIAEAAMLAGLPKAPSAYNPIANPKRATIRQLHIIDRM
                     LENGFITPAQREEARNQRLAYRTPSETPTHGEYAAEAARQLIYAQYGEDSYTLGLHVH
                     LTVKSSDQMVAYHALRRGILDYERRQFYRGPEAYIDLPADSHEVDTRIAEALTEHPDN
                     DELKAAVVLEVNPKKLVAVLLSGESVTVTGDGLKPVTSGLGDKGNPKTQIRRGAVVRL
                     TKNANGSWTATQLPEVEGAFIALSPRTGAIQAMVGGFDYSKTKFNHVTQAWRQPGSSF
                     KPFVYSAALEKGFSPATVINDAPLFFDAGSTGSQPWEPKNYDGTFDGPMSMRRGLAKS
                     KNMISIRILQSIGPTYAQEWATRFGFDADKHPAYLTMALGSGSVTPLQMASAYSVFAN
                     GGYRVNPYLISKVTDSKGRVVREVKPPVLDESMRTINPRNAFVMNSLLQEVTRSGTAA
                     RAQAALKRTDIYGKTGTTNDALDAWFAGYQHDVVAVVWMGYDNPRKLGDRETGGGLSL
                     PIWIDYMNVALKGRPENEPLPPEGVVNIGGEWYYEEYVQGGGVSSLGLEDHAPAAPNE
                     EERKGILDLFNR"
     gene            3927..4964
                     /locus_tag="PKV56_15145"
     CDS             3927..4964
                     /locus_tag="PKV56_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOX69195.1"
                     /translation="MIGLDISSSSVKLVELGQDESGAYILERFASESFEKGWITDGQI
                     EKFDEVADAVRRVIAKSGSKTKDVVMAMPQSSVITKKIMLPAGLRDEELEIQVESEAN
                     QYIPFSLDEVSLDFCVIGPSQTSVGDVEVLIAASRKDRVQDRQGLAEAAGLRPVVLDI
                     ESHASRLAMSRVVGALPNEGRDALVALFEIGADTTSLKVLRDEEMLYDRDQAFGGSQL
                     TQLISRQYGFSFEEAEQKKLAADLPDDYEQQILSPFVDSLSQEIGRALQYFFTSTPHH
                     KVHYVMLAGGTATLPGLKDRVTELTGFASMVVNPFDNMKLGGAVRESKLRREAPSYLT
                     ACGLAMRRFAQ"
     gene            4961..5605
                     /locus_tag="PKV56_15150"
     CDS             4961..5605
                     /locus_tag="PKV56_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOX69196.1"
                     /translation="MILINLLPHREEKRRRKKIAFLVGIGASAIAGALTVGIWYGIVQ
                     QLTSTQQERNAYLSSEIKKLEEQIKDVASLKAEIESLKARQKAVEDLQTDRNVPVYLL
                     NELVKQTPEGMYFSSVKQNGQTLTVLGIAQTNERVSEFLRNTANNSEWLEKPQLVEIK
                     VSGAQAADRSQKRLFDFSIRVTVKRPQEKPIPAAAPAASAAVPRTAALPAMAKG"
     gene            5613..6308
                     /locus_tag="PKV56_15155"
     CDS             5613..6308
                     /locus_tag="PKV56_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOX69197.1"
                     /translation="MANTTTSNVDLNSVFQGAASQFRGLNLNQPGQWPLLPKIAAWSV
                     IAIAVVVLGWFAVLATAHEELDAEVAKEPTLKQDYRNKLAQAVNLAELRKQKLQVEEY
                     VTQLEKQLPGKAEMDALLSDINQAGLGRGLQFELFRPGQVIVKDYYAELPIELKVSGR
                     FHDIGSFAADVANLSRIVTLHNLSIKGGKGGGDMLSLEATARTYRYLDAQEVQEMRKA
                     AAGKKGKAGGKKK"
     gene            6305..6844
                     /locus_tag="PKV56_15160"
     CDS             6305..6844
                     /locus_tag="PKV56_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOX69198.1"
                     /translation="MKGLYRFRTLAVSMGVLLLAACSAEQQELAQWMEQQRKEVKPNV
                     QPILPPKKFNPQAYLATDLVEPFSSQKLTVAIKQEARQPNSLLASEINRRPEPLESYP
                     LDSVSMVGSVTKQNKPFALLKVDKLLYQVKLGDYLGQNYGKIIKITETDVSLREIVQD
                     ASGEWTERISSIQLQEKAR"
     gene            6841..>8028
                     /locus_tag="PKV56_15165"
     CDS             6841..>8028
                     /locus_tag="PKV56_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HOX69199.1"
                     /translation="MKAMQEKRIMGGDWRARALTLALVLGAPLSAMAQAVIQSISSSQ
                     QAGTEVVRIELSEPLSAIPKGFVVQSPPRIAIDLPGVGNAMGKPTVDINQGNLRTVNV
                     AQSDDRTRVVLNLRQSSTYRAELQGKVLVLVLESSSSPAVASAVGAVEPVRFAEGQNA
                     QQLPLKDIDFRRGSDGAGRVIVDLPNNQVGVDIRQQGQSLVVEFLRSSLPDNLRRRLD
                     VTDFGTPVQTVSTVQSGDRVRMVVEPRGAWEHSAYQTENQFVLEVRPQKIDPNKLTQG
                     PGFAGEKLSLNFQNIEVRALLQVIADFTNFNVVTSDTVTGNVTLRLKDVPWDQALDII
                     LQAKGLGLRKSGNVILIAPKDELAAKEKVELESKAQIASLEPVRTQAFQLNYTKAEEV
                     ARGL"
BASE COUNT         1576 a         2376 c         2540 g         1536 t
ORIGIN      
        1 caaccaccga gatgcccggc aggctggctg cacgcgagta caccgccacc gcgcgctcgt
       61 gagcgatgcc gaacttgttg cccttcagtc ccgtggagat gtaggggtga gtcctggcat
      121 cgacgtccgg gttgacgcgc aggctgatgc gggcgcttcc accggactgt gtcgccacgg
      181 aagacagcgc ttccagttct gcttcgcttt cgacgttgaa gcacatcacc ccggcatcga
      241 gtgcctgacg catctcggcg cgggtctttc caacacccga gaagacaact ttcgacggtg
      301 tagctccggc cttcagtacg cgagccagtt cgccggcaga cacgatatcg aagccgcagc
      361 cggcttgcgc gaaggtctgc agcaccgcga ggttcgagtt cgccttgact gcgtagcaga
      421 tcaagtggtc gcgaccatcg agtgcccgtt ggtaggcgga cagtgccgac aacatcgctg
      481 cccgtgaata cgcatacagg ggcgtgccga aacgtgtcgc gaggtctgca actgcgcagt
      541 cctccagcat caacgtgtcg ccgcggtagg aaatctgggg tgagcccggc agggtcatgg
      601 cggaggactg ccccacgaac ggggcggtgg ggccgaggcg ggcgatgcga cagacgccgc
      661 cacggcgcgg ttggcgctgg ttgcagctgg agccttggga gccgagacga gtgtcagcgg
      721 ccccttctgg ccacatgccg acaacaacaa acccatggtc accaacgtgg acagcgcgaa
      781 gcaggcacgg gggctgggga ctaaactttc cgactgttgc gacatcgacc gaattctact
      841 gaccatgact accgactcga tgatcactcc cctgaccgac atggagttcc gcgccaaggc
      901 cggtgctgtg ctctcgcaca ttgaagtgac tttggatcgc tggctccagc aggacgtcat
      961 cgacatcgac gccagccgaa ccggggggct gctcgaaatg agcttcccca acggcagcaa
     1021 gatcgtcatc aacacgcagc cacccctgca ggagttgtgg ttggcagcac gcagcggtgg
     1081 atatcacttc aagcatgcgg acggccgttg gctcgacacg cgcggaggcc tcgagttcta
     1141 ctcggctctg tcggcttgca cgtccgagca ggccggggtc ggcctgagtt tcgaggcggc
     1201 ctgagcccgt ggtgaggtga atccggactt ccgggatctg cgcgccgggc tcagcgattg
     1261 aagagatcga gaatgccctt gcgctcttcc tcgttgggag cggcaggggc gtggtcttcg
     1321 agccccaggc tgctgacgcc accgccttgc acgtattctt cgtagtacca ctcgccaccg
     1381 atattgacca cgccctcggg cggcagcggc tcgttctccg gccgcccctt gagcgccaca
     1441 ttcatgtagt cgatccagat cggaagcgac aggccgcctc cggtttcccg gtcgcccagt
     1501 ttccgcgggt tgtcgtaacc catccagacc acagccacca catcgtgctg gtaaccggca
     1561 aaccaggcat cgagtgcgtc gttggtggtg cctgtcttgc cgtaaatgtc ggtgcgcttg
     1621 agtgccgcct gagcgcgcgc ggcggtgccc gagcgggtga cctcctgcag caggctgttc
     1681 atcacgaatg cgtttcgcgg gttgatggta cgcatcgatt cgtcgagaac cggcggcttg
     1741 acctctcgca cgacgcgccc cttcgagtca gtgaccttgc tgatcaggta gggattcact
     1801 cggtaaccgc cattggcgaa cacgctgtag gcggaggcca tctgcagagg tgtgaccgag
     1861 cccgagccca gcgccatggt caggtaggcc gggtgcttgt ctgcgtcgaa gccgaagcgg
     1921 gttgcccatt cctgcgcata ggtcgggccg atcgattgca ggatgcggat cgagatcatg
     1981 ttcttcgact tcgccagtcc tcttcgcatg gacatcgggc cgtcgaaggt gccgtcgtag
     2041 ttcttgggct cccagggctg gctaccggtg ctgcctgcat cgaagaacag cggcgcatcg
     2101 ttgatgaccg tcgctggtga aaagcccttt tcgagtgccg ccgagtagac gaaaggcttg
     2161 aagctcgacc cgggttgtct ccaggcttgc gtcacgtggt tgaactttgt cttcgaatag
     2221 tcgaacccgc cgaccatggc ctggattgcg ccggtgcgcg ggctgagcgc gatgaatgca
     2281 ccctcgactt ccggcagttg ggtggccgtc cagctgccgt ttgcattctt cgtcagacgc
     2341 actactgcac cacgccgaat ctgggtcttg gggttcccct tgtcgccgag gcccgacgtg
     2401 accggcttca ggccatctcc ggtcacggtg accgattcgc cactcaggag cacggcgacc
     2461 agtttcttcg ggttgacttc cagaacgacc gcagccttca gttcgtcgtt gtcagggtgc
     2521 tcggtcagcg cctcggcaat tcgggtgtcg acctcgtgcg agtcggctgg cagatcgatg
     2581 taagcctcgg gcccccggta gaactgccgg cgctcgtagt cgaggatgcc gcggcgcagg
     2641 gcgtggtacg caaccatctg atcgctggat ttcaccgtca gatgcacgtg caggcccagc
     2701 gtgtaggaat cctcaccgta ctgggcgtag atgagctgac gcgcggcttc ggccgcgtac
     2761 tccccgtgcg tcggggtttc cgagggcgtc ctgtaggcca gacgctgatt gcgagcctcc
     2821 tcgcgctgtg ccggagtgat gaacccgttt tccagcatcc ggtcgatgat gtgcaactgc
     2881 cggatcgttg cccgcttggg gttggcgatg gggttgtagg ccgacggcgc cttgggcagt
     2941 ccagccagca tcgccgcctc ggcgatcgac accgatttca agggctttcc gaaatagacc
     3001 tcgcttgccg acgcgaatcc gtaggcacgc tgcccgaggt agatctgatt catgtacagc
     3061 tcgaggatct ggtccttggt cagcaggctc tcgatcttca gtgccagcag gatctcgtag
     3121 atcttgcgag tgaacgtctt ttcggtggac aagtagaaat tgcgggccac ctgcatcgtt
     3181 atcgtggagg cgccctgact gcgcgattca ccgaagttgg ccaggccggc ccgcaggatg
     3241 cccaggtaat cgacgccgcc gtgctgatag aagcgggcgt cttcgatcgc cagcacggca
     3301 ttcttcatga ctgccggcat gtcggcggca gagacgaaat tgcgccgctc ctcgccgaac
     3361 tcgcccagca acacgccgtc ggctgcgtag atccgcatcg gcaacttggg tcggtaatcg
     3421 gtcaacccgc tgatctcggg cagattcggg taagcgaccg cgagggccag tgcgacgagc
     3481 atgagcaccg agagcgctcc ggctgccatc agaccgagcg cgccggtcac cgcgtggccg
     3541 atgctggtca gccaggatgg gggttgccgg cgtggcgatg cgggggcgga cctgccatcg
     3601 ccgcgcgggg tttcactcat gagcttccag gaatttcagg gccgattata ggaagtaggg
     3661 gggctccacc ccttttcaaa catgaatgga cacaactgca tcggagaaac tgctcatgtg
     3721 atctgcggaa ccaccgataa acaggtctga gaacgtgtgt aagccacaac gcggcacggg
     3781 cagccaagcc tgtttttcgt tggtgcacaa aggctttact gctaacattc aagtaacttc
     3841 ttaacagtcc accgctgttt tcagcgtgtg ggggggcatg gtctgtgagc tttctagaca
     3901 ccctgttagg ccgaaaacat cccccgatga tcgggctcga tatcagttca tcgagcgtca
     3961 aactcgttga attgggccag gatgaatcgg gcgcttacat attggaacga tttgcctccg
     4021 agagtttcga gaagggctgg attaccgacg gccaaatcga gaaattcgac gaagtggccg
     4081 acgcagtgcg tcgtgtgatt gccaagagcg gaagcaagac caaggacgtc gtgatggcca
     4141 tgccgcagtc gtcggtcatc accaagaaga tcatgttgcc ggctggtctg cgcgatgaag
     4201 aactggaaat ccaggtcgaa tccgaggcca atcagtacat ccccttctcg ctcgatgagg
     4261 tgagcctcga tttttgcgtg atcgggccga gtcagacgtc cgtgggtgac gtcgaggtgt
     4321 tgatcgcagc ttcccgcaag gatcgagtgc aggacaggca gggtctggcg gaagccgccg
     4381 gtctccggcc cgtggtgctt gacatcgagt ctcacgcctc gcgtcttgca atgagccgcg
     4441 tcgttggtgc tttgcccaac gaaggacgag atgccctggt cgccctgttc gagatcggcg
     4501 ccgacaccac cagtttgaaa gtccttcgcg acgaagagat gctttacgac cgagaccagg
     4561 ctttcggtgg ctcgcagctg acccagctga tttcccgtca gtatggtttc tcgttcgagg
     4621 aagccgagca gaagaagctg gctgcagacc tcccggacga ttacgagcaa cagatacttt
     4681 cgccgttcgt cgacagcctg tctcaggaga tcggacgggc tctgcagtat ttcttcacga
     4741 gcacaccgca ccacaaggtg cattacgtga tgctggccgg tggcaccgcc accttgcccg
     4801 ggctgaaaga tcgcgtcacc gaattgacgg gattcgcgtc gatggtcgtg aatccgttcg
     4861 acaacatgaa gctcggcggt gccgtccgtg agagcaagct gcggcgcgaa gcgccttcgt
     4921 acctgaccgc ttgtggtctg gcgatgagga ggtttgccca gtgatcctga tcaacctgct
     4981 tcctcaccgg gaggaaaagc gccggcgcaa gaagattgcc ttcctggtcg ggatcggcgc
     5041 gtccgccatt gccggagccc tgactgtcgg catctggtac ggcatcgtgc agcagctcac
     5101 ctccacgcag caggagcgca acgcctacct gagcagtgag atcaagaagc tcgaggagca
     5161 gatcaaggac gttgcctcgc tgaaagcaga aatcgaatcc ctgaaggcgc ggcagaaggc
     5221 ggtcgaggac ctccagaccg accgcaatgt gccggtctac ctgctcaacg aactcgtcaa
     5281 gcagacgccc gaaggcatgt acttttcttc ggtgaagcag aacgggcaga cgctcaccgt
     5341 tctgggcatt gcgcagacca acgagcgcgt gtcggagttc cttcgcaaca ccgcgaacaa
     5401 ttcagagtgg ctcgaaaagc ctcaattggt cgagatcaag gtctccggtg cgcaggctgc
     5461 ggatcgttcc cagaagcgtc tgttcgattt ctcgattcgc gtgaccgtca agcgtccgca
     5521 ggaaaaaccc atccccgcag ccgcaccggc cgcctccgcg gcagttccgc gaacggcggc
     5581 tctgcctgcc atggcgaagg ggtgaattcg acatggccaa taccaccaca agcaacgtcg
     5641 acctgaactc ggtatttcag ggcgccgcct cccagttccg ggggctgaac ctcaatcaac
     5701 ccgggcagtg gccgctgctg ccgaagattg cagcctggtc tgtcatcgcc atcgctgtcg
     5761 tcgtccttgg atggttcgcg gtgctggcca ccgcgcacga ggaactcgat gccgaggttg
     5821 ccaaggagcc gactctcaag caggactatc gcaacaagct ggctcaagcg gtcaacctcg
     5881 ctgaattgcg caagcagaag ctgcaggtcg aggagtacgt gacccagctc gagaagcaat
     5941 tgcctggcaa ggccgagatg gacgcgctgc tttccgacat caatcaggcc ggtctgggtc
     6001 gcggcctgca gttcgagttg ttccgcccgg gtcaggtgat tgtcaaggac tactacgccg
     6061 aactgccgat cgagctgaaa gtgtcagggc gctttcacga tatcgggtca ttcgcggccg
     6121 acgtcgcgaa cctgtctcgc atcgtgacgc tgcacaacct gtcgatcaag ggcggcaagg
     6181 gtggtggcga catgctttcg ctcgaggcga cggctcggac ctatcgatac ctcgacgcgc
     6241 aggaggtcca ggaaatgcgg aaagccgcgg cgggcaagaa gggcaaggca gggggcaaga
     6301 agaaatgaag ggcctctacc gtttccgcac gttggctgtc agcatgggtg tgttgcttct
     6361 cgctgcgtgc agtgccgagc aacaggaact ggcgcagtgg atggaacagc agcgcaagga
     6421 agtcaagccg aacgtgcagc ccatccttcc gccgaagaag ttcaatccgc aggcttatct
     6481 ggccaccgac ttggttgagc ccttcagttc acagaagctc acggtcgcga tcaagcagga
     6541 agcgcggcag ccgaactcgt tgctggcatc cgaaatcaat cggcgcccgg agccactcga
     6601 gtcctacccg ctggacagtg tgtcgatggt gggtagcgtg accaagcaga acaagccgtt
     6661 cgccctcttg aaggtggaca agctgcttta tcaagtcaag ctgggcgatt acctcggcca
     6721 gaactacgga aagatcatca agatcaccga aaccgatgtg tctcttcgtg aaatcgtgca
     6781 ggacgcgtcg ggtgaatgga cggagcgcat cagctcgatc caactccagg agaaagctcg
     6841 atgaaggcca tgcaggagaa acgcatcatg ggtggtgact ggcgggcacg cgcactgacg
     6901 ctcgcgttgg tattgggcgc acctctctcg gcgatggcgc aagctgtcat ccagtcgatc
     6961 agcagttcgc aacaggctgg aaccgaggtc gtacgaatcg agttgtcaga gccattgagc
     7021 gcgattccga aaggattcgt ggtccagtcg cccccgcgga tcgccatcga cctgccggga
     7081 gtcggcaatg cgatgggcaa gccgacggtc gacatcaatc aaggcaacct gcgcacggtc
     7141 aatgtcgcgc agtccgatga tcgcactcgt gtggtgctga acctgcggca gtcctcgacc
     7201 tatcgagcgg aattgcaggg caaggtgctg gtgctggtgc tggaatcgag cagctcgcca
     7261 gccgttgcca gtgccgtcgg cgcggtcgaa ccagttcggt tcgcggaagg ccagaacgca
     7321 cagcagttgc cgttgaagga catcgatttc cgccgcggat ccgatggcgc cggtcgggtg
     7381 atcgtggacc tgccgaacaa ccaggtgggg gtcgacattc ggcagcaggg gcaaagcctg
     7441 gtggtcgagt tccttcgctc cagcctgccc gacaacctgc ggcggcgtct tgatgtcaca
     7501 gacttcggaa caccggtgca gaccgtgtcg accgtgcaaa gcggtgaccg agtacggatg
     7561 gtggtcgagc cgcgcggtgc gtgggagcac agtgcatacc agaccgaaaa ccagttcgtg
     7621 ctggaggtcc ggcctcagaa gatcgatccg aacaagctca cccaagggcc gggattcgcg
     7681 ggcgagaaac tgtcgcttaa cttccagaac atcgaagtgc gcgccttgct gcaggtgatc
     7741 gccgacttca ccaatttcaa cgtcgtgacc agcgacacgg taacgggtaa cgtgactttg
     7801 cgcctgaagg acgttccgtg ggatcaggcg ctcgacatca tcttgcaggc caagggcctc
     7861 gggcttcgca agtcgggcaa cgtcatcctg attgcgccga aggatgagct cgccgcgaag
     7921 gagaaggtcg agctcgaatc gaaggcccag atcgcttcac tggagccggt gcgcacacag
     7981 gccttccagt tgaactacac gaaggctgaa gaggtggcgc gcgggctc
//
LOCUS       DAOORV010000148         7999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11157_length_7999_cov_6.670569, whole genome shotgun sequence.
ACCESSION   DAOORV010000148 DAOORV010000000
VERSION     DAOORV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_7999_cov_6.670569"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..480
                     /locus_tag="PKV56_15170"
     CDS             148..480
                     /locus_tag="PKV56_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69200.1"
                     /translation="MATDDPLSFRILLYRYLFFGWLFQDVNRGNVFERSAAWRHNLDR
                     APWLVTYLRRWIALGIVSFALGMLVEHGLCAQALSAFFYVPSVLSVVVNGVIGVLIAA
                     FKLLPAPF"
     gene            complement(487..1773)
                     /gene="hpnE"
                     /locus_tag="PKV56_15175"
     CDS             complement(487..1773)
                     /gene="hpnE"
                     /locus_tag="PKV56_15175"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOX69201.1"
                     /translation="MERKSNSSLAIVGGGWAGLAAAVEATRRGHDVTLFEMAPAPGGR
                     ARSVDTGEPVLDNGQHILIGAYRETLRLMREVDVDPHAAFLRSPLALVDSQGTGLRLA
                     RGPAVPAFALGVLSHTGWKWRERVALLTKALKWAAAGFDCDSSLTVGELCRALPTSVL
                     RDLIEPLCVAALNTPSMHASGRVFLRVLRDAMFSGRGSADLLLPRQPLGDLLPAPAAR
                     WLTQQGAQVLTSRRVMAIDPDGAGWRVDDIHFDGVVLACTASEAARLAQPHAPQWADL
                     AASFRYEPIVTVYALCAGTRLPQPMTLLHSSGNDGPAQFVFDHGWLNGRHGLFSFVIS
                     AAQHWVEAGTEATAEATFDQARRQLGPHLKAKLQTLRVFNEKRATFRCTPDLHRPPQR
                     VAQGLLAAGDYVEGPYPSTLEGAVQSGLSAAREWRA"
     gene            complement(1774..2622)
                     /gene="hpnD"
                     /locus_tag="PKV56_15180"
     CDS             complement(1774..2622)
                     /gene="hpnD"
                     /locus_tag="PKV56_15180"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HOX69202.1"
                     /translation="MTPEQYVQDKAARSGSSFYYAFLFLPPARRAAITAFYAFCREVD
                     DVVDETTDAGVAASKLAWWRSEVASAYAGQPSHPVMKALMPHSATFAIQASHLQAVID
                     GCQIDLEQTRFLDFPGLSRYCHLVAGVVGEVAANIFGRSSEATVQYAHRLGLAMQLTN
                     IIRDVGDDARRGRIYLPVSELQRFDVKASELLLRTAPYGYSDRFSALMRFQAERAHQT
                     YDEAMALLPEVDRLAQKPGLMMANIYRSLLREIEIGGFQVLHQRTSLTPLRKLWIAVR
                     THWQGR"
     gene            complement(2619..3473)
                     /gene="hpnC"
                     /locus_tag="PKV56_15185"
     CDS             complement(2619..3473)
                     /gene="hpnC"
                     /locus_tag="PKV56_15185"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOX69203.1"
                     /translation="MSVDHYENFPVASWLCPPALRASVGAIYWFARTADDLADEGDAS
                     AAQRLADLAAYRADLNAVAAHHAPSPRWAAVFTPLARAMDEHDLPLSMLADLLSAFEQ
                     DVVKTRYADRAELLDYCSRSANPVGRLLLHLYGVRDNASRVQSDAICSALQLINFWQD
                     LSIDTPRNRLYMPLSDAAAHGVDAQTLLDRRDSAEVHRLIASVTHWARTLMLQGAPLV
                     HAVPGRAGWELRLVVQGGLRIADKIEAMDYATLLRRPHIGKGDAPALLWRALRMRRPV
                     GSLTRETA"
     gene            3608..3694
                     /locus_tag="PKV56_15190"
     tRNA            3608..3694
                     /locus_tag="PKV56_15190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3642..3644,aa:Leu,seq:tag)
     gene            3735..5042
                     /gene="tig"
                     /locus_tag="PKV56_15195"
     CDS             3735..5042
                     /gene="tig"
                     /locus_tag="PKV56_15195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOX69204.1"
                     /translation="MAVTVETLEKLERRITLTLSAETIQSEISNRLKKLSRTVKADGF
                     RPGKVPMSVVAQRYGYSVQNEVMNDQIGQAFADAANEAKLRVAGAPRITEKEGAESGT
                     MMFDAVFEVFPEVKIGSLEGVEVERYHTDITEAAIDRTIDILRKQRRTFAPRPAGEPA
                     VETDRVTIDFEGKFDGVPFEGGTAQGFQFVIGEGQMLETFDLAVRGMKVGESKTFPLR
                     FPDDYQNKEAAGKEADFLVTVNAIEVQQLPELTDMFARSLGILDGSMESLRKDVRSNL
                     EREVKARVMTRNKGSVMEVLLKNSELDVPAALVDDEAKRLVESTIADMKQRGAKDMDK
                     AQIPTKIFQPQAERRVRLGLVVSELVRANNLQAKPEQLQAYIEEMAQSYEKPSEVVSW
                     YLGDKQRMADVEVVVVENNVAEFVMSRGKAIDKDVPFDELMAS"
     gene            5207..5815
                     /gene="clpP"
                     /locus_tag="PKV56_15200"
     CDS             5207..5815
                     /gene="clpP"
                     /locus_tag="PKV56_15200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOX69205.1"
                     /translation="MSAIHTQGLGMVPMVIEQSGRGERAYDIYSRLLRERVIFLVGPV
                     NDQSANVVVAQLLFLESENPDKDISFYINSPGGSVSAGMSIFDTMQFIKPDVSTLCMG
                     IAASMGAFLLAAGAKGKRFALPNSRVMIHQPSGGAQGQATDIEIQAREILKLRESLNG
                     ILADRTGQPLEKIRADSERDYFMSSEEAKDYGLIDQVISKRS"
     gene            5929..7197
                     /gene="clpX"
                     /locus_tag="PKV56_15205"
     CDS             5929..7197
                     /gene="clpX"
                     /locus_tag="PKV56_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOX69206.1"
                     /translation="MADKKGSSSEKVLYCSFCGKSQHEVKKLIAGPSVFICDECIELC
                     NDIIRDEVPAESADAKATKSDLPVPSEIKASLDQYVIGQEPAKRTLAVAVYNHYKRLK
                     HMSRNKDDVELSKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGED
                     VENIIQKLLQNCNYDVEKAQRGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLV
                     EGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFDGLEKVIQNRSEKSGIGFGANV
                     KSKVDRKVSEVFREAEPEDLIKFGLIPELVGRLPVMATLGELTEDALVQILTEPKNAL
                     IKQYQKLFSMDGVELEIRPSALTAIARKALARKTGARGLRSIMEQSLNDTMFELPSFE
                     GVVKVVLDEHTIEDEAKPLLVYREQAKASA"
     gene            7291..>7999
                     /locus_tag="PKV56_15210"
     CDS             7291..>7999
                     /locus_tag="PKV56_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOX69207.1"
                     /translation="MSGHPVLPAEPITLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     SAMEAGRQIMLVAQKAAGKDEPKPDDMFDVGCVSTILQMLKLPDGTVKVLVEGLQRAN
                     THSITDNGEYFVSEVTPMPPEVETKPEIEALRRAVTQQFDQYVKLNKKIPPEILTSIA
                     GIDDAGRLADTIAAHLPLKLENKQSVLDLFAVDKRLEKLLEQLEHEVDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQ"
BASE COUNT         1592 a         2446 c         2508 g         1453 t
ORIGIN      
        1 gtcaggacac tgttggcgcg ggtctgaacc ggggaatgcg gaaggggctc aagtccggag
       61 agtttcgcgc cgatatcgga gccaacgcgg gtcacgcatc gttcgaagcg tgccgatcca
      121 ccagcgtttg gcaggcagat ccgacacatg gccaccgacg accccctctc tttccgcatc
      181 ctgctttatc gctacctgtt cttcgggtgg ctcttccagg atgtgaaccg tggcaacgtg
      241 ttcgagcgct cggcggcctg gcgccataac ctcgaccgag ccccttggct ggtgacctat
      301 ctgcgccgat ggattgctct gggcatcgtt tcgtttgcac tgggcatgct ggtcgaacac
      361 gggctctgtg cgcaggcact gtcggcgttc ttctatgtgc ccagcgtgct cagtgtcgtc
      421 gtcaacggtg tgatcggcgt gctgatcgcc gccttcaagc tgctgcctgc gccgttctga
      481 gcgttctcag gcgcgccatt ccctcgctgc gctcaagccc gactgcacgg cgccctccag
      541 ggtggacggg tagggtcctt cgacgtaatc gcccgcggcc agcaggcctt gagcgactct
      601 ctgtggcggc ctgtgaaggt ctggtgtgca gcggaacgtc gcccgtttct cgttgaagac
      661 gcgcaatgtc tgcagcttgg ccttgaggtg cggtcccaac tgtctgcgtg cctgatcgaa
      721 cgtggcctct gccgtggctt cggtgcctgc ctcgacccaa tgctgtgctg cgctgatcac
      781 gaagctgaac agaccgtgcc ggccattcag ccatccgtga tcgaaaacaa actgtgccgg
      841 tccgtcgttg ccgctcgaat gcagcaatgt catcggctga ggcagtcgtg ttccggcaca
      901 cagcgcatag acggtgacga tcggctcgta gcgaaaactc gcggcgagat cggcccactg
      961 cggcgcatgt ggctgggcaa gtcgcgcagc ttcgctggct gtgcaggcca acacgacccc
     1021 gtcgaagtga atgtcatcga ctcgccaccc agctccgtcg ggatcgattg ccatgacccg
     1081 tcgcgacgtc aggacttgcg ctccctgttg cgtcaaccag cgtgcagccg gcgcaggcaa
     1141 cagatcgccg agtggctgtc gcggcagcag cagatcggcc gaaccccggc cggagaacat
     1201 cgcatcgcgc agcacgcgca ggaagactcg cccactggcg tgcattgaag gcgtgttgag
     1261 cgccgcgaca cacaagggct cgatcaggtc gcgcagtacc gaggtgggta gcgcacggca
     1321 caactcgccg acagtcagac tggaatcgca gtcgaagccg gccgccgccc acttgagcgc
     1381 cttcgtcagc agcgcaaccc gctcacgcca tttccagccc gtgtgcgaca gcactccaag
     1441 cgcgaatgcg gggacagccg gacctcgggc aagtcgcagt ccggtcccct gggagtccac
     1501 cagcgccagc gggcttctca agaaggccgc atgggggtcg acatcgacct cccgcatcag
     1561 gcgcagcgtc tcgcggtaag ccccgatgag tatgtgctga ccgttgtcca ggaccggttc
     1621 accggtgtcg acgctgcgcg ctcttccacc gggtgccggt gccatctcga agagcgtcac
     1681 gtcgtgacct cgtcgtgtcg cctcgacagc ggccgcgagc ccggcccagc ccccgccgac
     1741 gatggcgagg ctcgagttcg acttgcgctc caatcagcga ccctgccagt gcgtgcgaac
     1801 ggcgatccac agcttgcgta acggcgtcaa cgaggtgcgc tggtgcagca cctggaatcc
     1861 accgatttcg atctcgcgca gcagggatcg gtagatgttt gccatcatca gccccggctt
     1921 ctgcgccagc cgatcgactt ccggaagcaa tgccatcgcc tcgtcgtaag tctgatgcgc
     1981 gcgttcggcc tggaagcgca tcaacgcgga gaagcggtcg ctgtagccgt atggcgcggt
     2041 gcgcagcagc aactcgctgg ctttcacatc gaaacgctgc agttcagaga ccggcaggta
     2101 gatgcggccg cggcgggcgt cgtcacccac gtctcggatg atgttggtga gttgcatcgc
     2161 cagacccagc cggtgcgcat attgaacggt cgcttcgctc gatcggccga atatgttggc
     2221 cgctacctcg ccgaccaccc cggccaccag atggcagtag cgggacaggc ccggaaaatc
     2281 cagaaagcgc gtttgctcca ggtcgatctg gcagccgtcg atcaccgcct ggagatggct
     2341 tgcctggatg gcaaaggtgg cgctgtgcgg catcagcgcc ttcatgaccg ggtgactggg
     2401 ctggccggcg taggcacttg ccacctcgct gcgccaccac gcgagcttgg aggccgccac
     2461 gccggcatcc gtcgtttcgt ccaccacatc gtcgacctct cgacagaacg catagaaggc
     2521 agtgatcgcc gcgcggcgcg ccggtggcag gaagaggaag gcgtagtaga agctggagcc
     2581 gctcctggcg gccttgtcct gtacgtactg ttctggtgtc atgcggtctc gcgggtgagt
     2641 gagccgaccg ggcgacgcat tcgcaacgca cgccaaagca gggcaggggc gtctcctttg
     2701 ccgatgtgcg gccgccgtag cagcgtggcg taatccatgg cttcgatctt gtcggcgatg
     2761 cgcagcccgc cctggacaac cagccgcagt tcccatccgg cccggcctgg caccgcatgg
     2821 acgaggggcg ctccctgcag catgagagtc cgggcccagt gcgtgaccga agcgatcagg
     2881 cggtgaactt cggcgctgtc tcgccggtcg agcagcgtct gcgcatcgac accatgcgct
     2941 gctgcgtcgc tcaggggcat gtacaggcga tttcgcggtg tgtcgatgct caggtcctgc
     3001 cagaaattga tcaattgcag cgcactgcag atcgcatccg attgaacccg cgaagcgttg
     3061 tcgcgcacgc cgtagaggtg cagcagaagg cgcccgaccg ggttggccga gcggctgcag
     3121 tagtcaagca attcagcgcg gtcagcgtac cgggttttca ccacgtcctg ttcgaatgcg
     3181 ctgagcaggt ctgccagcat cgacaacgga aggtcgtgct cgtccatggc ccgcgccagc
     3241 ggtgtgaaga ccgcagccca gcggggggag ggcgcatggt gggctgcgac tgcattcaga
     3301 tccgcccggt aggcagcaag atcggccagg cgttgcgcag cgctggcgtc gccttcgtcc
     3361 gccagatcgt ccgccgttcg cgcgaaccag taaatagccc cgaccgaggc gcgcagagcc
     3421 gggggacaca accaggaagc gacaggaaaa ttttcgtagt gatcaacgct cacggcgcgc
     3481 attgtccatg catcgttgcg tgaagtcgtt tccagcggaa gccggcgcgg ttaaactcgc
     3541 gggctttctg cggtgcgcag aattccgttt gatagaagcg cgtgctcctg tcgtttgaat
     3601 tggccccgcg cgggtggcga aattggtaga cgcaccaggt ttaggtcctg acgccagcaa
     3661 tggtgtgcgg gttcgagtcc cgccccgcgc accagccaca gcaatcagca acgtctacat
     3721 aaggtctctt gatcatggca gtgactgtcg aaacccttga aaagctcgaa cggcgtatca
     3781 cgctgaccct gtccgccgag acgattcaga gcgagatttc caatcggctg aagaagctgt
     3841 cgcggaccgt gaaggctgac ggcttccgcc ccggcaaggt gccgatgagc gtggtggccc
     3901 agcgctacgg ctactcggtt cagaacgagg tgatgaacga ccagatcggg caggcattcg
     3961 ccgatgcggc caacgaggcc aagctgcggg tcgccggagc gccccgcatc accgaaaagg
     4021 aaggcgcgga atcgggcacc atgatgttcg acgcagtctt cgaggtcttc cccgaggtga
     4081 agatcggcag cctcgaaggc gtggaggttg agcgatatca caccgacatc accgaagccg
     4141 ccatcgatcg cacgatcgac atcctgcgca agcagcgtcg cacattcgcg ccgcgccctg
     4201 caggcgaacc agcggtcgag acggatcgcg tgacgatcga cttcgagggc aagttcgatg
     4261 gagtgccttt tgaaggcggg acggcgcagg gcttccagtt cgtgattggc gaaggccaga
     4321 tgctcgagac gttcgacctc gcggtgcgtg gcatgaaggt gggcgaatcg aagaccttcc
     4381 cgctgcgctt cccggacgat taccagaaca aggaagcagc cggcaaggag gccgacttcc
     4441 ttgtcacggt caacgcgatc gaggtccagc aactgcccga gttgaccgac atgttcgcgc
     4501 gcagtctggg tatcctggac ggatccatgg aaagcctgcg caaggacgtc cgcagcaacc
     4561 tcgagcgcga ggtgaaggct cgcgtgatga cgcgcaacaa gggctcggtg atggaggtcc
     4621 tgctcaagaa cagcgaactg gatgttccag ccgccttggt cgacgacgag gccaagcggc
     4681 tggtcgaatc gacgattgcc gacatgaagc agcgcggcgc caaggacatg gacaaggcac
     4741 agatcccgac caagatattc cagccccagg ctgagcgtcg tgtccgcctg ggcctggtgg
     4801 tgagcgaact ggttcgtgcc aacaaccttc aggccaagcc cgagcagttg caggcatata
     4861 tcgaggagat ggcacagagt tacgagaagc cctccgaagt cgtcagctgg tatctgggcg
     4921 acaagcagcg gatggcggat gtcgaggtgg tcgtggtcga gaacaatgtc gccgaattcg
     4981 tgatgtcacg cggcaaggcg atcgacaagg acgttccatt cgacgagttg atggcgtcct
     5041 gatcgccttc ggcctgcgcg caggggcacc gagccgcaca ggccggtgcc cctgcgcatt
     5101 tccacacccc catcgggatc gcgttgtgac gacataattc gctctcgtct tcccgctggc
     5161 atgggccgca gagataagcc agagaacgat aactaggaca tcacgcatga gcgcaattca
     5221 cacgcaaggc ctgggcatgg tcccgatggt catcgagcaa tccggccgcg gcgaacgcgc
     5281 ctacgacatc tactcgcgcc tgctgcggga gcgggtgatc tttctggtcg gtccggtcaa
     5341 cgatcaatcc gccaacgttg ttgtcgcgca actgcttttc ctggaaagcg agaacccgga
     5401 caaggacatt tccttctaca tcaattcgcc tggcggctcg gtcagcgccg gcatgtcgat
     5461 cttcgacacc atgcagttca tcaagccaga cgtctccacg ctttgcatgg gcatcgccgc
     5521 gagcatgggt gccttcctgc tggctgccgg tgccaagggc aagcggttcg ccttgccgaa
     5581 ttcccgggtg atgatccatc agccttcggg cggtgcgcag ggacaggcaa cggacatcga
     5641 gatccaggcc cgcgaaatcc tcaagctgcg cgagagcctg aacggcatcc tggctgaccg
     5701 caccggacag ccgcttgaaa agatccgcgc cgattcggag cgcgattact tcatgtcgtc
     5761 ggaagaagcc aaggactatg ggttgatcga ccaggtgatt tccaagcggt cctgacgcgc
     5821 tgaagcgttc gatcagcgta ttcaacgggg cctttcttca cggcaaggcc ccgtttcctt
     5881 ttgttctatt attcagacag cactttcgct gaccggtggc acatttccat ggccgacaaa
     5941 aaaggctctt cgagcgaaaa agtcctgtat tgctccttct gcggcaagag ccagcacgag
     6001 gtcaagaagc tcattgcagg cccctcggtt ttcatctgcg atgagtgcat cgagctttgc
     6061 aacgacatca tccgcgacga ggttccggct gaaagtgcgg atgccaaggc gaccaagtcc
     6121 gatctgcccg tcccgagcga aatcaaggcc agcctggacc agtacgtgat tggtcaggaa
     6181 ccagccaaac gcaccttggc ggtggcggtc tacaaccatt acaagcggct caagcacatg
     6241 agccgcaaca aggacgacgt cgagttgtcc aagagcaata tcctgttgat cggcccgacc
     6301 gggtccggca agacgttgtt ggcgcagacg cttgcgcgtc tgctcaatgt gccgttcgtg
     6361 attgcagacg cgaccacgct gaccgaagcg ggctatgtgg gtgaggatgt cgagaacatc
     6421 attcagaagc tcctgcagaa ctgcaattac gacgtcgaga aggcacagcg cggcatcgtc
     6481 tatatcgacg agatcgacaa gatctcgcgg aaggccgata acccttcgat cacgcgtgac
     6541 gtgtccggag aaggcgtgca gcaggccttg ctcaagttgg tggagggcac gatggccagc
     6601 gtgccgccgc agggcgggcg caagcatccg aaccaggatt tcctgcagat cgacaccacg
     6661 aacatcctgt tcatctgcgg cggtgccttc gacggccttg aaaaggtcat ccagaaccgg
     6721 tccgagaagt ctggtatcgg gtttggtgcc aacgtgaaga gcaaggtcga tcgcaaggtc
     6781 agcgaggttt tccgagaagc cgagcccgag gatctgatca agttcggtct catacctgaa
     6841 ctcgtcggac gactgccggt gatggctact cttggcgaat tgactgaaga cgctctggtt
     6901 cagatcctga cggagcccaa gaacgccttg atcaagcagt accagaagct cttcagcatg
     6961 gacggcgtcg aactggagat ccggccatcg gcgcttactg cgatcgcacg caaggccctg
     7021 gctcgaaaga cgggagctcg cggattgcgc tcgatcatgg agcaatcgct caacgacacg
     7081 atgttcgaac tgccttcgtt cgagggtgtc gtgaaagtcg ttctggacga gcacaccatc
     7141 gaagacgaag ccaaacctct gctcgtctat cgcgagcaag ccaaggccag cgcctgaact
     7201 accggtctgc cgcgcaggtg acttcccctc gcacggctcc ttgcaatcaa aaactcaagc
     7261 cctagatagg cttgagaaag agaggtttcc atgtccggac atcctgtact ccccgctgag
     7321 cccatcacgc tgccgttgct gcccctgcga gatgtggtgg tgtttcccca catggtgatt
     7381 ccgctgttcg tcgggcgccc gaagtcgatc aaggccctcg agtccgcgat ggaggcaggg
     7441 cgccagatca tgctggtggc gcagaaggct gccggcaagg acgagccgaa gccggatgac
     7501 atgttcgacg tcgggtgcgt ctcgaccatc ctgcagatgc tgaagctgcc cgatggcacc
     7561 gtgaaggtgc tggtcgaagg actgcaacgc gccaacaccc acagcatcac tgacaacggc
     7621 gagtacttcg tctccgaagt cacgccgatg ccgccggaag tggagaccaa gcccgagatc
     7681 gaggccctgc gtcgcgccgt cacccaacag ttcgaccagt acgtcaagct caacaagaag
     7741 atcccgccgg agatcctgac ttcaatcgct ggcatcgacg atgcgggtcg ccttgcggac
     7801 acgatcgcgg cacatctgcc gctgaagctc gagaacaagc aatcggtgct cgatctgttc
     7861 gcggtcgaca agcggctgga gaaattgctc gagcagcttg agcacgaagt agacatcctc
     7921 caggtcgaga agcgcatcag gggtcgcgtc aagcgccaga tggagaagag ccagcgcgag
     7981 tactacctga acgaacagg
//
LOCUS       DAOORV010000149         7966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11216_length_7966_cov_12.360258, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000149 DAOORV010000000
VERSION     DAOORV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11216_length_7966_cov_12.360258"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKV56_15215"
     CDS             complement(<1..773)
                     /locus_tag="PKV56_15215"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOX69208.1"
                     /translation="MGTRKTSTGQAPPGARRRAVREALSGDGATAGAGVEAAFAEVVG
                     LIHAARQRTAQAVNTELIDLYWRIGQFLHLRIEADGWAQGTVVQLAAYVARREPGQRG
                     FSAQNLWRMRQFFLAYPAPAALSTPLRALPWSSHLHILSRTKRPEEREFYLRMAAQQR
                     WQVREVARQIDSGLFERAVLDPPKLSTALRQLQPQAEQHFRDVYQLDFLALPDDHSEA
                     DLHRGLLQNLGRFLTELGRDFCYIGSQYPVQVGGQDFAL"
     gene            complement(785..2164)
                     /locus_tag="PKV56_15220"
     CDS             complement(785..2164)
                     /locus_tag="PKV56_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HOX69209.1"
                     /translation="MPDQGRLFPIEVDDEHLLKHVNAIALMPVPGGRRISQLGRRLFN
                     VLLHRAQETGEQDEYQARLYEIVDDAGYNSNDTAPIRKILRELMSTTVEWQSPTNGEI
                     ETWDACNLLSGAGTTKDKRTGAVTVRWRYDSKVRAQLLSPDRYARLSLEAITQLSTHA
                     AMALYEICARYVDNPGHLTARQHWRWWRPVLTGVHSDNAKAEYRFFKRDVLHKAIAEI
                     NACTNIEVRGPIEYKEKDNRTIAEIQFEVHAKGDMPIRRQPVPLANLVVDDLPLIGRA
                     LQAGVKQGEAEELIQRHGPAQLAAGLDELEKRLKMPSEKVGAVLKPGSWLRANVARAA
                     KLAPEPQAVAEPIVSNESLQKHRAAWTDEWLRRRKDRLRKGFQELAQADQQEHMDAFR
                     QELKATGQLQLLKRLDISGWQHRMVLAGFTKFYGIRVLGDDWDKPSPDDILAIAAEQA
                     MAVQLPPTS"
     gene            2823..4001
                     /locus_tag="PKV56_15225"
     CDS             2823..4001
                     /locus_tag="PKV56_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69210.1"
                     /translation="MNQELMLEPQSLSDIMEQADRAGYLMSEVRGAILAPARRKTTPT
                     FNATQLAALCDVDRSQVVYRLSKNDLPAGKINQSGNRREFTLEETQAWVREYRVKRLR
                     PKGAEAICISVANFKGGVGKTTTAMSLAQGLSLKGHRVLLIDCDPQGSLTTLFGIMPD
                     TEVDESQTILPVCLGQEASIEPAIQKTYWNNLDLVCAAPLLFAAEFGLPARQMREEGF
                     EFWNALNIALDDVRERYDVIILDTPPALSYVTINALMASDGILMPLPPNALDAASAAQ
                     FWRLFSDLTGDLVMHRGVSKEFDFVRVLLTRVDSQDSTAAVVREWIGKTYEGKVLPAE
                     IPKTTVASSSAAEFGTVYDISRYEGNQRTYKRALDAYDKVTELLEEIIRASWRRHLAA
                     "
     gene            4013..5095
                     /locus_tag="PKV56_15230"
     CDS             4013..5095
                     /locus_tag="PKV56_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX69211.1"
                     /translation="MVKKAALKTAGISMAGFQPRAKVFDGQTPEPTASTPDATREHGR
                     PFTGVGSVMAAITREAEISQELIGAQAKLQQATVKLAEYEGADLVRALDPKAVRRSQW
                     ANRAEAEFSTPEFLLLKDEIANAGGNVQPIKVRALEGAPTVFDGQTPTHEIVFGHRRH
                     QACLELGLAVNAIVVERMDDKSMFEAMDRENRGRKNLSAWEQGRMYDQAIKQGLYPSL
                     RRLSESLGVNLSDASRAVHLAKLPKEVIAAFNSPLDLQVRWAKPLVDALQRDPDGVLK
                     RARKCALQTAPRGPAEVFAQLVGSESKPALHDVVIANGKQRLAILRLASNGKATIEFE
                     AGVLTDDRRGPLAQVLKDFLLGSKPN"
     gene            complement(5543..7357)
                     /locus_tag="PKV56_15235"
     CDS             complement(5543..7357)
                     /locus_tag="PKV56_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69212.1"
                     /translation="MSVAEAAARYLGIDHGLQARSAHAAAIDTVRALARRVGQRDWRL
                     IGLVIGVPATTGAGAAQSGARPSLEEFIVERELHDWSEAEAREFYEEAFPVDKRAQRR
                     YRLRERQLQLLRTLETSSAVAAAPHDRIDTWFDPVTSQRLGSVGLLLLSDLAERIALR
                     GRWWQAIPAVGAGKAARIAAFLKVLLPAWSPPAPGGGRGARSSFALAGAAVALSEASS
                     TAAPSSSPPSAALAPSPALPRATSSPHAIQAPAQAPEGALLAARTDADAMESWIDARA
                     GSEATARSYRREARRLLLWLHLERARGLRTMTVDDCRGYAAFLEHVPPAWISRAKAQP
                     LADGWAPFRGPLSVGSRRLSLIVLASFFDWLVAAGYLPRNPWALINRKIGDTQERAAE
                     QLDTRAFTPEAWAAILGMIDAEPPSPARARMQFILRFVEATGLRSAELVSARLAQLRR
                     HRGGWVMAVHGKGDKTRVVAVPEQARQALEAYLAARQLPSIERAPEEAPLLASTADPM
                     ETVTYSALYKTVKVWLHRAIDASPLQGRDRELALRASPHWLRHTFGTRAVERDVPLEV
                     VQRQLGHADPRTTSRYSRAQLERLQAAMDRAFGGNETA"
BASE COUNT         1443 a         2471 c         2627 g         1425 t
ORIGIN      
        1 tcgagggcga agtcctggcc gccgacctgc accggatact gcgatccgat gtagcagaag
       61 tcgcgcccca actcggtcag gaagcgtccc aggttctgca gtaggccgcg gtgcaggtcg
      121 gcttcggagt ggtcgtcggg cagcgccagg aagtccagct ggtagacgtc gcggaagtgc
      181 tgctcggcct gcggctgcag ttgtctcaac gctgttgaga gttttggtgg gtcgagcaca
      241 gcacgctcga acaggccgct gtcgatctgg cgcgccacct cacgcacctg ccagcgctgc
      301 tgcgcggcca tgcgcaggta gaactcgcgc tcttccggcc gcttggtgcg gctgaggatg
      361 tgcaggtgcg acgaccaggg caaagctctc aacggcgttg agagtgctgc cggcgccgga
      421 taggccagaa agaactgccg catacgccag aggttctggg ccgagaaccc gcgctggcca
      481 ggttcacgcc gggcgacata ggcggccagc tggaccaccg tgccttgggc ccagccatcg
      541 gcctcgatcc tgaggtgcag gaactggccg atgcgccagt agaggtcgat gagctcggtg
      601 ttcaccgctt gcgcagtgcg ctggcgcgcg gcgtggatca ggccgacaac ttcggcgaag
      661 gctgcctcga cgcctgcgcc ggcagtcgcc ccatcgccag agagcgcttc acggacggca
      721 cggcgtcgcg cgccaggtgg ggcctgaccg gtggaagtct tgcgcgtgcc cacgctgatg
      781 accttcagga agtcgggggc agttgaactg ccatggcctg ttcggcggca atcgccaaga
      841 tgtcgtctgg cgaaggcttg tcccagtcat cgcccaaaac acggatgcca tagaacttgg
      901 tgaaccccgc cagcaccatg cggtgctgcc accccgagat atccaggcgt ttgagcagct
      961 gaagttgtcc agtcgccttg agctcttgtc gaaacgcgtc catgtgctct tgttgatcgg
     1021 cctgcgcaag ctcttgaaag cccttgcgca accgatcctt ccgccgacgc agccattcgt
     1081 cggtccaggc tgcgcggtgc ttctgcaggc tctcgttgct gacgatgggc tcggcgactg
     1141 cctgcggctc tggcgccagc ttggccgccc gtgcgacatt cgcccgcaac caactgcctg
     1201 gcttaaggac cgcccccacc ttctcgctcg gcatcttcag ccgcttctct aattcatcca
     1261 ggcccgcggc cagctgtgcc ggcccgtgcc gctgaatcag ttcttcggcc tcgccctgct
     1321 tgacgcctgc ttgcaaggcc cgcccgatga ggggcaggtc atcgaccacc agattggcca
     1381 gaggaacggg ctgcctgcgg atgggcatgt cgcccttggc atgcacctcg aactggatct
     1441 cggcgatggt gcggttgtcc ttctccttgt attcgatggg cccgcgcact tcgatgttgg
     1501 tgcaagcgtt gatctcggca atggccttgt gcagcacgtc ccgcttgaag aagcgatatt
     1561 cggccttggc gttgtcactg tggacaccgg tgagcactgg ccgccaccag cgccaatgct
     1621 ggcgcgccgt caagtgccca gggttgtcga cataccgggc acagatttca tacagcgcca
     1681 tcgctgcatg cgtcgacagc tgggtgatgg cctccagcga gaggcgtgca tagcggtcgg
     1741 ggctcagcaa ctgcgcacgc accttcgagt cgtatcgcca gcggacggtg acggcgccgg
     1801 tccgcttgtc cttggtggta cctgccccgc tcaacaggtt gcaggcgtcc caggtttcaa
     1861 tctcgccgtt cgtcgggctc tgccattcga cggtggtcga catcagctcg cgcaggatct
     1921 tccggatggg cgctgtgtcg ttgctgttgt agcccgcgtc gtcgacgatt tcgtacagac
     1981 gcgcttggta ctcatcctgt tcgccagtct cctgtgccct gtgcagcagc acgttgaaca
     2041 ggcgccggcc cagctgtgag attcgccgtc cgccagggac gggcatcaac gcaatggcat
     2101 tgacgtgctt gagcaggtgc tcgtcgtcaa cttcaatcgg aaagaggcga ccttgatccg
     2161 gcatgcgaaa tcatagcagc gtgtgtgagc ttcgcaccac aacggttctc acgttcgaaa
     2221 gctgggttat gagtcctgta gcgcctcacg tgaaagaggg gcctctggcg gcacgctttg
     2281 ctcgccgaac gcttcgctga actggtgtag gaaattcatg cccgctttga gccgctggcg
     2341 taccaggcga cagactccgt ggtgcctggg agaggtcacc atggcgcgag gggcctcact
     2401 gggagccttg tgtgagtgga cacaggggtg agcgcggaga accccatcat ccccgtgtcc
     2461 actcactcat gcccctgtca caactcacgt tcagtcctat gggagctcac gttgagccct
     2521 gtaccgcctc acagataccc ccgtagctcc tcacgtatcc tgcttcaact gcttgtcagc
     2581 attgaagatt tccattcgca aagggtttga atgggttttg aggttttgaa gtatttcaaa
     2641 caccgcaaag gctcttgaag acgccaacca acccactggc ggctgctcag ccggcgttcg
     2701 aggtgtgcag gtgccgcaag cacaaagaaa gtcgctagtg aacgcctcaa ttttttgctt
     2761 tactggagaa agctagcaca atcgaagcat ctcagcacta cgccgcttta acggctacga
     2821 cgatgaatca agagctgatg cttgagccac agtccttgtc ggacatcatg gagcaggcag
     2881 atcgcgcggg ctacctgatg tcggaggtcc gcggagccat cttggcacct gccaggcgca
     2941 aaaccacgcc gaccttcaac gcgactcagt tggcagctct gtgcgacgtt gatcggagcc
     3001 aggtcgtcta ccgactcagc aagaatgacc tgccggcggg caagatcaac caaagcggca
     3061 atcgacgcga gttcacgctg gaagagaccc aggcgtgggt ccgagagtat cgggtgaagc
     3121 gcttgcgacc aaagggggcg gaagcaatct gcatctcggt cgccaacttc aagggtgggg
     3181 ttggcaagac gaccaccgcc atgagcttgg cacagggtct atcgctcaag ggccatcggg
     3241 tcttgttgat cgactgtgat cctcaggggt cgctgacaac cctcttcggc atcatgccgg
     3301 acacagaagt ggatgaatcg cagacgattc ttccagtatg cctggggcaa gaggcgagca
     3361 ttgagccagc aatccagaaa acatactgga acaatctaga cttggtctgt gcggctcccc
     3421 tgctctttgc agcagagttc gggctgcccg cccgacagat gcgcgaggaa ggattcgagt
     3481 tctggaacgc gttgaacatc gcgctggacg atgtgcggga gcggtacgac gtgatcatcc
     3541 ttgacacccc ccctgccctg tcttacgtca cgatcaatgc attgatggcg agcgatggca
     3601 tcctgatgcc gctgccgccg aatgcactcg atgcggcgtc cgccgcgcag ttctggcgct
     3661 tgttttccga tctcacaggt gatttggtca tgcaccgtgg cgtcagcaag gaattcgact
     3721 ttgtccgggt gctgctcact cgggttgaca gtcaggactc gaccgctgca gtggtgcgcg
     3781 agtggatcgg caagacctac gaaggcaagg ttcttccagc agagatcccg aaaaccacgg
     3841 tggcctcctc cagtgctgcc gagttcggaa ctgtctacga catcagccga tacgagggga
     3901 accagcgtac gtacaagcgc gcacttgatg cttacgacaa ggtcaccgag ttgctcgaag
     3961 agatcattcg agcatcctgg cgcaggcatc tggccgcttg aggagaagag aattggtcaa
     4021 gaaggcagca ttgaaaacgg ctggcatcag catggccggc ttccaaccca gagccaaggt
     4081 gtttgacggt caaacaccgg aaccgaccgc ttcgacaccc gatgccacca gagagcatgg
     4141 gcggccgttc accggcgtag gcagtgtgat ggcagcaatc acacgcgagg ccgagatctc
     4201 gcaggaattg atcggtgccc aagccaagct ccagcaggcg accgtgaagt tggcggagta
     4261 cgagggtgcc gacttggtca gggcattgga tccgaaggct gtgcgtagga gccaatgggc
     4321 aaatcgcgca gaggcggagt tcagcacgcc agagtttctg ttgctgaaag atgaaattgc
     4381 caacgcaggc ggcaatgttc agcccattaa ggtgagggcg ctggagggag ccccgacggt
     4441 gtttgacggt caaacaccta cccatgagat cgtcttcggg caccgtcgcc accaggcctg
     4501 cctggagttg ggcctggcag tcaacgcgat cgtggtcgag cgcatggatg acaagtccat
     4561 gttcgaggcc atggacaggg aaaaccgagg ccggaagaac ctgagtgctt gggagcaggg
     4621 ccgtatgtac gaccaggcga tcaagcaagg gctctaccca agcttgcgcc gcctgtcgga
     4681 aagcctaggc gtgaatttgt ctgacgcgtc ccgtgctgtt cacttagcca agctgcccaa
     4741 ggaggtcatt gccgccttca actcgccgct cgatcttcaa gtgcgatggg ccaagccact
     4801 ggtcgatgct ttgcaacggg atccagacgg cgtgctgaag cgtgcacgca agtgcgcgtt
     4861 gcagaccgcg cctaggggcc cagccgaggt tttcgctcag ttggtcggct cggagtcaaa
     4921 acccgctctg catgacgtag tcatcgccaa tggaaagcag cgactcgcaa tcctgcgctt
     4981 ggcgtcgaac ggcaaggcaa ccattgagtt cgaagcgggt gtgctgacgg atgaccgccg
     5041 gggtcctttg gcccaggtgc tgaaggactt cttgttgggg tcaaagccaa actgaaggca
     5101 ttgccatagg tgtcttgccg ggcgggggcg gatggttggt cactcaaccc gcatggctag
     5161 ggcgccaggg tggcctatgg ggcattgaat gccgcctgaa gctggtgatg actgcgcgcc
     5221 ttctcgctgc gcacgtagat cgatgtggtg gtcagttcgg ccaggcgcat gcccgccatc
     5281 gagccgaact ccagcaagaa gtgcagtcgg tgcagggcag gggagggcgc tgggcaggcc
     5341 cgtcgtgcgc aacgactgca cgacctcatt tgtcgcagct tcctgggtga cgacggattg
     5401 gcgcgccgtg tcggcgaagg cttcgacgag gtgttcacgg ggtcggaggc gcccaaggcg
     5461 ctagtgtttc ctgggccctc agtcgcagta cggtcttcgt gacggcccac gacgaccggc
     5521 tacagccctg gagccccggg tgctaagccg tctcgttacc gccaaacgct cggtccatgg
     5581 ctgcctgcag tcgctccagc tgcgcccggc tgtagcggct cgtcgtacgc ggatcggcgt
     5641 ggccaagctg gcgttgcacg acctccagcg gcacgtcacg ctcgaccgcg cgggtaccga
     5701 aggtgtgccg cagccagtgc ggcgaggccc gcaaagccag ctccctgtcg cggccctgca
     5761 gcgggctggc gtcgatggca cgatgcagcc agaccttcac cgtcttgtac agcgccgagt
     5821 aggtgacggt ctccatggga tcggcggtgc tggccagcag cggcgcttcc tccggggcgc
     5881 gttcgatgga aggcagttgc cgtgcggcca gataggcttc cagcgcctgc cgagcctgct
     5941 ccggcacagc caccacccgc gtcttgtcac ccttgccgtg caccgccatg acccagccgc
     6001 cccggtgacg acgcagctgc gccagccggg cgctcaccag ctcggccgaa cgcaggccag
     6061 tggcctcgac aaagcgcagg atgaactgca tgcgagcccg ggcaggggag ggtggttcag
     6121 cgtcgatcat gcccaggatg gcggcccacg cctccggggt gaaggcccgg gtgtcgagtt
     6181 gctcagccgc gcgctcctgg gtgtcgccga tcttccggtt gatcagcgcc caagggttgc
     6241 gaggcagata tccggccgcc accagccagt cgaaaaagct cgccagcacg atcaacgaca
     6301 ggcgccgact gcccaccgac agtggccccc ggaagggcgc ccagccatcg gccagcggct
     6361 gcgccttggc gcgcgagatc cacgctggcg gcacgtgctc caggaacgcg gcgtagccac
     6421 ggcagtcgtc gaccgtcatc gtgcgcaggc cccgggcgcg ctccaagtgc agccagagaa
     6481 gcaaccgcct ggcctcacgc cggtagctgc gggcggtggc ctcagagccc gcgcgggcgt
     6541 cgatccagct ttccatggca tccgcgtcgg tgcgtgcggc cagcagagcg ccttcagggg
     6601 cttgcgcagg cgcctgaatg gcatgcgggc tgctcgtggc gcgaggcagg gcaggggagg
     6661 gcgccagggc agcgctcggc gggctggaag agggggcagc ggtggatgac gcctcgctca
     6721 gcgcaactgc tgcgccggcc agcgcaaaac tcgaccgcgc gccgcgcccg cctcccggcg
     6781 ccggtggcga ccaggcgggc aacaacacct tcaggaacgc ggcgatgcgg gccgccttgc
     6841 cggcgccgac ggcagggatg gcctgccacc agcgcccgcg cagcgcgatg cgctcggcca
     6901 ggtcgctcag cagcagcagc ccgacgctgc ccaggcgctg actggtcacc ggatcgaacc
     6961 aggtgtcgat gcggtcgtgc ggcgcggctg cgaccgcgct cgaggtctcc agtgtgcgca
     7021 gcagctgcag ctggcgctcg cgcaggcggt accggcgctg cgcccgcttg tcgaccggga
     7081 aagcctcctc gtagaactcg cgggcttccg cttcgctcca gtcgtgcagc tcgcgttcaa
     7141 cgatgaactc ctccagcgac ggtcgggcac cggattgtgc agcgcccgca ccggtggtgg
     7201 cgggcacgcc gatgaccaga ccgatgaggc gccagtcacg ctgaccgacg cgccgggcga
     7261 gggcgcggac ggtgtcgatg gctgcggcat gcgccgagcg tgcctgcaag ccatggtcaa
     7321 tgcccaggta ccgcgccgcg gcctcggcaa cgctcaaacc ctcggagaca gcgcgcaggt
     7381 gggcgaagtg atgccggccg acttggagaa ctcgatcagc gccacgcttg cccagctggc
     7441 gcctgcttgg gctgttctca ggtcgcgcgc cttgcgccga agccgcggca tgggcgtcga
     7501 gaacggcgtc cggaagagcg gtcggcggcg cgtaagtcct tgtcggcact ggggaacggg
     7561 agagggcagg gcgggggccc cttttctccg tggatttcta agctacagat tatcagcgta
     7621 atctgtagct atggaagctt gcagatcggc atcgccaccg aatgggggca gcgggtaggg
     7681 cacgccacgc ttgcagatca gggcagggcg ccgcagggcc gcgcacggag cccacgcgtc
     7741 gtgaaaagga ggttcatcgg gctacaaagc tgacctcgaa ggcctgtcgg tgcttctacg
     7801 gccccgagca ccgggcacgc ctgaaaggag gcgcagcact tgcgcggctg gtgcgcatgc
     7861 cgtttcaccg gggcatgttg tccaccgtgc cggtggataa gcatgtgcac gagcccaggg
     7921 cagggcgctg caggccgcgc cgatcctcgc tgtcaagacc ggtgct
//
LOCUS       DAOORV010000150         7866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11409_length_7866_cov_8.444245, whole genome shotgun sequence.
ACCESSION   DAOORV010000150 DAOORV010000000
VERSION     DAOORV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11409_length_7866_cov_8.444245"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKV56_15240"
     CDS             <1..344
                     /locus_tag="PKV56_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOX69213.1"
                     /translation="IPELNKKLTGMSFRVPTSDVSVVDLTVELSKEASYKEICAEMKA
                     QSEGAMKGVLGYTEDKVVSTDFRGEPMTSVFDADAGIALDSTFVKIVAWYDNEWGYSN
                     KVLEMVRVVAK"
     gene            479..826
                     /locus_tag="PKV56_15245"
     CDS             479..826
                     /locus_tag="PKV56_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69214.1"
                     /translation="MNRVPLALTAFVICLAACAAAPPNLPADATDGLRVLVELAPEAT
                     SGGASDERPASVERRATASAGVPVRYVAASGARWHALMLICSPTECDAALQRLTADRA
                     GFLVVQRDELRRR"
     gene            852..2540
                     /locus_tag="PKV56_15250"
     CDS             852..2540
                     /locus_tag="PKV56_15250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOX69215.1"
                     /translation="MRLQSSIARRVRCICSLALALPAVFATGAAMAVPEPTARVIVKF
                     RVGVDAAATSAEQLPARVGGVARRLGLPVRQGLALADRTQLLMASGLSSQQFAARLAQ
                     DSAVEYAVPDELRHRAAPPNDPRYAQGLSAPGPAAGQWYLRAPASAVRSAINIEAAWA
                     VVAGGPNIVVAVLDTGIRYDHPDLKRAADGGNLLDGYDFVTNGFIENDATPGRDSDAT
                     DPGDWVSSDDVISPSNTIGCTTSEIGDSSWHGTQTSGLIGALTNNGVGMAGLTGPGRA
                     LRVLPVRVLGKCGGFDSDIIPAMRWAAGLVVPGVPANPTPARVINLSLGGTGACNAAY
                     QDAVREINQAGVVVVASAGNTTGHAVNTPANCAGVIGVGGLRHLGSKVGFSDLGPEIT
                     ISAPGGNCVNLTGDCLYPLLTTTNAGVTTPVPSSEAYTDATNATIGTSFSAPLVAGTA
                     ALMLAVGPGLSPDEIRSLLRRSSRPFPTSGDSASVPTCQAPQVDSTGEPIDQFECYCT
                     TSTCGAGMLDAGAAVALSKADDDKDGGGGALGLGWLLALGVAIRAVAATRRPQA"
     gene            complement(2574..3065)
                     /locus_tag="PKV56_15255"
     CDS             complement(2574..3065)
                     /locus_tag="PKV56_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69216.1"
                     /translation="MLRWVVLALLLANLAFFALTRGQALRAEREPDRHAQQVHPEAVR
                     LLTPASVAAASAASAAEAASAAEAADASASSAIATRCFEAGPFNTAEVAVAEAALSAS
                     PAASALAQRWSRVTADVPGAPSAASAVHRLRIDAATPQESAALEALPANLLIHAFAPC
                     ALR"
     gene            complement(3059..4024)
                     /locus_tag="PKV56_15260"
     CDS             complement(3059..4024)
                     /locus_tag="PKV56_15260"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOX69217.1"
                     /translation="MNNSSTMISESGLQWNVEQLYHQLAPRLPNLSVEVVSQIPSTNT
                     ALLERARIATRLSSEGDLAQVRRSTESLAFGRRTADHQPCLLVAEHQTEGRGRQGRVW
                     QSEAGASLTFSLAVPLAPVDWSGFSLAVGVALADALDPTGTSTSTPWIGIKWPNDLWL
                     MPPGGTANSGRKLGGLLIETVPAGALRLAVIGVGLNVLPLSIDRSQVASGFACLEELD
                     RQATAPQVLARIALPLVEALQRFEREGFAGFAERFAARDVLRGCAVQAGAGLSDPARA
                     ATGVHGVAEGVSGNGALRLRVLDELREVIAGDVSVRLQPALDRPC"
     gene            complement(4021..4509)
                     /locus_tag="PKV56_15265"
     CDS             complement(4021..4509)
                     /locus_tag="PKV56_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOX69218.1"
                     /translation="MTQVTVGKSLHRSSGRRIQVRRSSVHGKGVFAVAPIGRNESIIE
                     YTGEVIDWPEALRRHPHDPTDPNHTFYFSLEGGWVIDAKHGGNSSRWINHACEPNCQA
                     DEVDGRVFIRALRPLKPGDELFYDYGLVLDERQTAKLKKQYACHCGSRHCRGTMLAPK
                     AR"
     gene            4662..7283
                     /locus_tag="PKV56_15270"
     CDS             4662..7283
                     /locus_tag="PKV56_15270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOX69219.1"
                     /translation="MTKTLIIAEKPSVAQDIVRALTPVAGKFDKQADHFENERYVVTS
                     AVGHLVEIKAPEEFDVKRGKWSFNNLPVIPPHFDLAPIDKAKSRLSAVVKLVKRKDVT
                     DLINACDAGREGELIFRLIVQYAGSEKKPIDKPIRRLWLQSMTPQAIRDGFEKLRGDT
                     EMQGLSDAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSVVVEREE
                     KIRKHVSRPYWEVRATFAAVAGEYDGKWFDPKWKKNPDDPELRNDRVWNESDAQAIAQ
                     AVRGEPASVTEEAKPSSQSAPGLYDLTTLQREANSRFGFSAKTTLSIAQALYEKHKVL
                     TYPRTDSKALPEDYLPVVRDTMAMLAGEELPGPLRPLSAHAGKALKEGYVKPNKRIFD
                     NAKVSDHFAIIPTLQAPKSLTEIEAKLYDMVVKRFVAVFYPPAEFMVTTRISMVQAAG
                     KDFSFQTNGKVMVKPGWLAVYGREAQEDDANLVAVAPGEVVRTESVDVNPLKTKPPAR
                     YTEATLLSAMEGAGKLIDDDELREAMAEKGLGTPATRAQTIEGLILEKYIVREGRELI
                     PTAKAFQLMTLLRGLQVEELTKPELTGNWEFQLAEMEKGKLSRETFMAEIAKMTERVV
                     AKAKEYDRDTIPGDYATLAVPCPNCGGVVKENYRRFTCIGKDGAVSGDSGEGPGCGFS
                     ISKIPGSRSFELPEVEAFIADKKIGPLEGFRSKAGWPFTAELKLVFDDEIKNWKLEFD
                     FGEDAKAEAGEPVDFSEQEPLGHCPKDQGKVYEHGTSYVCEHSVGANVTCDFKSGKII
                     LQQPVPREQMAKLLQTGKTDLLENFVSNKTRRKFKARLAFDAKEGKVSFEFEPRPVKP
                     AAGKAPAAKKAAAKKAS"
     gene            complement(7297..>7866)
                     /locus_tag="PKV56_15275"
     CDS             complement(7297..>7866)
                     /locus_tag="PKV56_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69220.1"
                     /translation="SRNERNANAGIVVGINPQDYRQDGQSGGPVNPLDGMAFQRSWES
                     RAFELGDGGYAAPAQLVGDFLRGQASSVLGSVEPSYKPGVHLTDLGRRDRSSLPLYAI
                     DAIREALPAFEGQIKGFSMPDAVLTGVETRTSSPLRITRGRDHQSLNVRGLYPAGEGA
                     GYAGGIMSAGVDGIEVAESVGRSMLGLKD"
BASE COUNT         1417 a         2559 c         2611 g         1279 t
ORIGIN      
        1 tgattcctga actcaacaag aagctgaccg gcatgtcgtt ccgcgtgccc acctctgacg
       61 tgtcggtcgt ggatctgacg gtcgagttga gcaaggaagc aagctacaag gaaatctgcg
      121 ccgagatgaa ggcacagtcc gaaggcgcga tgaagggggt gctcggctac accgaggaca
      181 aggtggtctc gaccgatttc cgcggcgagc cgatgacgtc ggtgttcgac gccgatgcgg
      241 gcattgcgct cgacagcacg ttcgtcaaga tcgtggcctg gtacgacaac gagtggggct
      301 actcgaacaa ggtgctcgag atggtgcgag tggtggcgaa gtaaggctcc tcatccccaa
      361 ccgcgaaacg gcccctttcc ggggccgtct ttcatggcgg gcgtttctga cgaaccttga
      421 tgtggtcgac aagttcgctt cacatccaag acacggcccg gcgcctagac tcgacccgat
      481 gaaccgagtc ccgcttgctt tgacggcgtt cgtgatctgc ctcgcagcct gcgcggcagc
      541 gccgccgaac ctgcccgccg atgcgaccga tggcctgcgc gtgctggtgg agctggcgcc
      601 cgaagctaca tccggcggcg cgtccgacga gcgacccgct tcggtcgagc ggcgtgccac
      661 ggcgagtgcg ggagttcctg tgcgatatgt tgctgccagc ggcgctcgat ggcacgcatt
      721 gatgctgatc tgctctccca ccgaatgtga tgctgcgctg caaaggttga ccgccgaccg
      781 cgcaggtttt ctggtcgttc agcgcgacga gcttcgccgt cgctgatgac tcctcaaccg
      841 gaacccccac catgcgactc cagtcttcca ttgcgcgccg ggtgcgttgt atttgcagcc
      901 ttgccttggc gctgcctgca gtattcgcga ctggcgccgc aatggccgtg ccggagccca
      961 cagcgcgtgt gatcgtcaag ttcagggtgg gcgtcgatgc tgcagcgact tcggcagagc
     1021 aactgccagc acgggtgggc ggcgtggccc gtagattggg cttgcccgtg aggcagggcc
     1081 tggcacttgc cgaccgcacg caattgctga tggccagtgg cctcagttcg cagcaattcg
     1141 ccgcccggct ggcgcaggac agcgccgtcg aatatgcggt gcccgacgag ctgcgccacc
     1201 gcgcagcccc gccgaacgac ccgcgctatg cgcaggggct gagtgccccg ggaccggcgg
     1261 ccggtcagtg gtatctgcgc gccccagcca gtgcggtgcg gtcagcaatc aatatcgaag
     1321 ctgcctgggc cgtcgttgct ggcggcccga acatcgtcgt cgcggttctg gacaccggca
     1381 tccggtacga ccaccccgat ctgaagcgcg cggccgatgg aggcaacctg ctcgacggct
     1441 atgacttcgt gaccaacggc ttcatcgaga acgacgccac acctggccgc gacagcgatg
     1501 cgaccgatcc cggcgactgg gtgagctccg atgacgtgat cagcccgtcc aacaccatcg
     1561 gctgcacgac ttcggagatc ggcgacagtt cctggcacgg cacgcagacg tctggcctga
     1621 tcggcgcact caccaacaac ggggtcggca tggccggcct gaccggcccc ggtcgggcac
     1681 ttcgggtgct gccggtgcgt gtgctcggca aatgcggcgg attcgattcg gacatcattc
     1741 cggcgatgcg ctgggccgcc ggcctcgtcg tgccgggagt tcccgccaac ccgacgccgg
     1801 cgcgcgtcat caacctcagt ctcggtggca cgggagcgtg caacgcggcc taccaggacg
     1861 ccgttcgcga gatcaaccag gctggcgtcg tggtggtggc atctgccggc aacacgaccg
     1921 gccatgcggt caacacaccg gccaactgcg caggggtgat cggcgtcggt ggcctgcgtc
     1981 acctcggctc gaaggtgggc ttctccgacc tcggtccgga gatcacgatc agcgcaccgg
     2041 gcggcaattg cgtcaacctg accggcgact gcctctaccc cttgctgacg acgaccaacg
     2101 ccggcgtcac cacgccggtg ccatccagcg aggcctacac cgatgcgacc aacgccacga
     2161 tcggcaccag tttctcggcg ccgctggtgg cgggcactgc cgcgctgatg ctggctgtcg
     2221 ggcccggtct ctcgcctgat gaaattcgca gcctgctgcg ccgatcgtcg cggcccttcc
     2281 caacctcggg cgacagcgcg agtgtgccaa cctgccaggc tccgcaggtc gacagcaccg
     2341 gggagccgat cgatcagttc gagtgctact gcaccacttc gacctgtggc gccggcatgc
     2401 tcgatgccgg cgctgccgtc gcactctcga aagccgacga tgacaaggac ggaggcggcg
     2461 gagcgctcgg tctgggctgg ctgctggcgc tgggcgtggc gatcagggcc gtggcggcca
     2521 cgcgacgacc gcaggcctga tcggagtctg gtctgcgctt cgtgtcctgg atgtcagcgc
     2581 agtgcgcagg gggcaaatgc gtggatcagc aggttggctg gcagggcctc gagcgcggcc
     2641 gattcctgcg gcgttgccgc gtcgatccgc aggcgatgca ctgcggaggc cgcactcggc
     2701 gcgccgggta catcggcagt gacccgagac cagcgctgtg ccagggcact ggcggccggg
     2761 ctggctgaca gagcagcctc cgccacggcc acttccgccg tgttgaacgg accggcctcg
     2821 aagcagcgag tggcgatagc cgacgatgcg ctggcgtccg cagcttcagc cgccgaggcg
     2881 gcctctgcag ccgatgccgc gctggccgcc gcgacgctgg ccggcgtcag caggcgcacg
     2941 gcttcggggt gaacctgctg ggcatgtcga tcgggctccc gctcggcacg caacgcctgt
     3001 ccacgtgtca acgcgaagaa agccaggttg gccagcagca gggccagcac cacccatctc
     3061 agcacgggcg atccagtgca ggttgcagcc gcacgctgac gtcaccagcg atcacttcgc
     3121 gcaattcgtc gagcacgcgc aaacgcaagg ccccgttgcc ggaaacgcct tcggccacac
     3181 cgtggacacc ggtcgccgct cgcgctggat cagacaggcc cgcgcctgcc tgcaccgcac
     3241 aacctcgcag cacatcgcgt gctgcgaacc gttcggcgaa gcctgcaaat ccctcgcgtt
     3301 cgaagcgctg caatgcttcc acgagaggca gggcgatacg cgccagcacc tgcggcgcag
     3361 tggcctgtcg gtcgagctct tccaggcagg cgaagccgct cgcgacctgg ctccggtcga
     3421 tggacagcgg cagcacgttg agacccaccc cgatgacagc caggcgcagt gcaccggccg
     3481 gcaccgtttc gatcaacagg ccgccgagct ttcgtccgct gttggcggtt ccaccgggcg
     3541 gcatcagcca caggtcgttg ggccacttga tgccgatcca cggcgtcgat gtcgacgtgc
     3601 cggtcgggtc cagcgcatcc gcgagcgcga cgcccactgc cagcgagaag cccgaccagt
     3661 cgacaggtgc cagcggcacc gccagcgaga acgtcagtga cgcgcctgcc tcggactgcc
     3721 aaacgcgccc ctgtcgaccg cggccctcgg tctggtgctc ggccaccagc aggcagggct
     3781 ggtgatcggc ggtgcgccgg ccgaaggcga ggctctcggt gctgcgccga acctgggcga
     3841 ggtcaccttc gctagacagg cgcgtggcaa tccgggcgcg ttcaagcagc gcggtgttgg
     3901 tcgacggaat ctgcgagacc acctcgacgc tgaggttggg cagcctgggc gccaactggt
     3961 gatagagctg ctccacgttc cactgaaggc cgctttcgga gatcatcgtc gaggagttgt
     4021 tcatcgcgct ttcggtgcca gcatcgtccc tctgcagtgg cggctgccgc aatggcaggc
     4081 gtactgcttc ttcagcttgg cggtctggcg ctcgtcgagc accagaccgt agtcgtagaa
     4141 caattcatcg ccgggcttca gcggccgcag cgcgcggatg aacacgcgac cgtcgacttc
     4201 gtcggcctgg cagttgggct cgcaggcgtg gttgatccag cgcgaactgt tgcccccgtg
     4261 cttggcgtcg atgacccagc caccctccag gctgaagtag aaggtgtgat tcggatcggt
     4321 cggatcgtga gggtggcgac gcagggcctc gggccagtcg atgacctcgc cggtgtattc
     4381 gatgatcgat tcgttgcggc cgattggcgc gacggcgaag acgcctttgc cgtggacgct
     4441 gctgcgacgc acctggatgc gtcggcctga agagcgatgg agagactttc caacggtcac
     4501 ttgcgtcatc gacttctttg ggtacattga aattgtgggc gcgcgagtgc gtgtgcccgt
     4561 gcgcatgcgc gcgagatgtc gaattgtaga gacgtgtgct gctgactaga agaggtacac
     4621 gtcgaagctt gcgccaaagt taagccttcc ttgaaagaaa catgaccaag accttgatca
     4681 ttgccgagaa gcccagcgtc gcgcaggaca tcgtgcgcgc tctcacaccc gtcgcgggca
     4741 agttcgacaa gcaggccgac cacttcgaga acgagcgtta cgtggtcact tcggccgtcg
     4801 gccacctggt ggagatcaag gcgccggagg agttcgatgt caagcgtggc aaatggagct
     4861 tcaacaacct gccggtgatc ccgccgcact tcgatctggc gccgatcgac aaggccaagt
     4921 cgcggctctc cgccgtggtg aagctggtca agcgcaagga cgtgaccgac ctcatcaatg
     4981 cctgcgacgc gggacgcgag ggcgagctga tcttccggtt gatcgtgcag tacgccggta
     5041 gcgaaaagaa gccgatcgac aagccgatcc gccggctttg gctgcagagc atgacaccgc
     5101 aggccatccg cgacgggttc gagaagctgc gcggcgacac cgagatgcag ggcctgtccg
     5161 acgcggcgcg cagccgcagc gaggccgact ggttggtggg catcaacggc acccgcgcga
     5221 tgaccgcctt caattcgcgc gacggcgggt tcttcctgac caccgtcggg cgcgttcaga
     5281 cccccaccct gagcgtggtc gtcgaacgcg aggaaaagat ccgcaagcac gtctcccggc
     5341 cttactggga ggtccgggcc acgttcgctg cggttgctgg tgaatacgat ggcaagtggt
     5401 tcgacccgaa gtggaagaag aaccccgacg atcccgagtt gcgcaacgat cgggtctgga
     5461 acgagtccga cgcgcaggcg atcgcacagg cggtgcgtgg cgaacccgct tcggtcacgg
     5521 aagaggccaa gcccagctcg cagagcgcac ccggtctgta cgacctcacc acgctgcagc
     5581 gcgaggcgaa ctcccgattc ggtttcagtg ccaagacgac gctgagcatc gcgcaggcgc
     5641 tgtacgagaa gcacaaggtg ctgacctatc cccggaccga cagcaaggcc ctgcccgagg
     5701 actacctgcc cgtggtcagg gacacgatgg cgatgctggc cggcgaggaa ctgcccggcc
     5761 cgctgcgccc cctgtccgca catgcgggca aggcgctgaa ggagggctac gtgaagccca
     5821 acaagcgcat cttcgacaac gccaaggtgt ctgaccactt cgcgatcatc ccgacactgc
     5881 aggcccccaa gagcctgacc gagatcgagg ccaagctcta cgacatggtg gtcaagcgct
     5941 ttgtggctgt cttctatcca ccggccgagt tcatggtcac cacgcgaatc tcgatggtgc
     6001 aggcggcggg caaggacttc agtttccaga ccaacgggaa ggtgatggtc aaaccgggct
     6061 ggctcgcggt ttacggccgc gaggcgcagg aggacgatgc caatctcgtc gccgtggcac
     6121 ccggtgaggt ggtgcgtacc gagagcgtcg acgtcaaccc gttgaagacc aagccgccgg
     6181 cacgctatac cgaggccacg ctgctcagcg cgatggaggg cgctggcaag ctgatcgatg
     6241 acgacgagct gcgcgaggcg atggccgaga agggactggg cacaccagcc acccgggcgc
     6301 agaccatcga agggctgatc ctcgaaaagt acatcgtgcg cgaaggtcgc gagctgatac
     6361 ccaccgccaa ggcgttccag ctgatgacgc tgctacgtgg cttgcaggtc gaggagctga
     6421 ccaagcccga gctcaccgga aactgggagt tccagctcgc cgagatggag aagggcaagc
     6481 tcagccgcga gaccttcatg gccgagatcg ccaagatgac cgagcgcgtc gtcgccaagg
     6541 cgaaggaata cgaccgcgac accatccctg gtgactatgc gacgctggcc gtgccgtgtc
     6601 cgaactgcgg cggagtcgtc aaggaaaact atcgccggtt cacctgcatc ggcaaggacg
     6661 gtgcggtttc cggggacagc ggtgaaggcc cgggctgcgg cttttccatc agcaagatcc
     6721 ccggcagtcg cagtttcgag ctgcccgagg tcgaggcgtt catcgcggac aagaagatcg
     6781 gtccgctcga aggcttccgc tcgaaggccg gttggccatt caccgccgag ctgaaactgg
     6841 tgttcgatga cgagatcaag aactggaagc tcgaattcga cttcggcgag gacgcgaagg
     6901 ccgaggccgg cgagccggtt gacttttccg agcaggaacc gttgggccac tgcccgaagg
     6961 atcagggcaa ggtctatgag cacggcacca gctacgtctg cgagcacagc gtaggcgcca
     7021 acgtcacctg tgatttcaag agcggcaaga tcatcctgca gcagccggtg ccacgcgagc
     7081 agatggccaa gctgttgcag accggcaaga ccgacctgct ggagaacttc gtgtcgaaca
     7141 agacccgccg caagttcaag gcgcgactgg ccttcgatgc caaggaaggc aaggtcagct
     7201 tcgagttcga gccgcgaccg gtcaagccag cagcgggcaa ggcaccggca gcaaagaagg
     7261 ccgctgccaa gaaggcgagc tagcagcgcc tgtagttcag tctttcagac ccagcatcga
     7321 gcggccgacc gattcggcca cctcgatgcc gtcgacaccc gccgacatga ttccgcccgc
     7381 gtagccggcg ccttcaccag ccggatacag gcctcgcacg ttcaggctct ggtgatccct
     7441 gcctcgcgtg atgcgcagcg gtgaagacgt gcgtgtctcc acgccggtca ggaccgcatc
     7501 gggcatcgag aaacccttga tctgcccttc gaacgccggc agcgcctcgc gtatggcatc
     7561 gatcgcgtaa agcggcaggc tgctgcggtc gcgccggccg aggtcggtca ggtgcacgcc
     7621 gggcttgtac gacggctcga cgctgccaag cacgctcgac gcctggccgc gcaggaaatc
     7681 gcccaccagc tgagccggcg ccgcgtagcc cccgtcgccc agttcgaacg cccgagattc
     7741 ccagctgcgc tggaacgcca tgccgtccag cgggttcacc ggcccgccgg actggccgtc
     7801 ctgccggtag tcctgcgggt tgatgccgac cacgatgccg gcgttggcgt tgcgctcgtt
     7861 gcgcga
//
LOCUS       DAOORV010000151         7831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11465_length_7831_cov_9.570216, whole genome shotgun sequence.
ACCESSION   DAOORV010000151 DAOORV010000000
VERSION     DAOORV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11465_length_7831_cov_9.570216"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKV56_15280"
     CDS             <1..548
                     /locus_tag="PKV56_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX69221.1"
                     /translation="MPADTVKKNIDKATGNLEGVHYDEIRYEGYGIGGAAVIIDTMTD
                     NKVRTVAEVRHVFSKYGGNLGTDGSVSFQFKHCGQIVFAPGTSEDTVMEIALEAGAED
                     VVTDDEGAIEVMTSPHDFEAVKAALEAAGLKPEVAEVTMRPENTVELAGEDALRMQKL
                     LDVIEDLDDVQAVFHNAELSE"
     gene            625..1932
                     /gene="purD"
                     /locus_tag="PKV56_15285"
     CDS             625..1932
                     /gene="purD"
                     /locus_tag="PKV56_15285"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOX69222.1"
                     /translation="MKVLVIGSGGREHAIAWKLLQSARVQTVYVAPGNGGTGSDNRLH
                     NVPLTDFKDLAEFAEREKIALTVVGPEGPLAGGIVDFFRARGLRIFGPTQAAAQLESS
                     KAYAKAFMKRHRIPTASYETFTESAAAHDYVDTHGAPIVIKADGLAAGKGVVVATTMA
                     QAHEAIDWMLGTDGAANTLGVQHNAGADGQAVPRVVIESFLEGEEASFIVMCDGKNVV
                     SLATSQDHKRLLDGDEGPNTGGMGAYSPAPVVTPNVHAKAMHEIILPTIQGMAKDGIP
                     FTGFLYAGLMIDAQGQPRTVEFNCRLGDPETQPILMRLKSDLFDVFMHATDGSLDEVE
                     LQWDRRVALGVVVAAHGYPYTPRKGDAIHGIPAEAADGIVFHAGTELRDGQLVTSGGR
                     VLCVTVLADSVKQAQPRAYELLHAIHFDGMQFRRDIGHRAVKR"
     gene            1945..2892
                     /gene="hemF"
                     /locus_tag="PKV56_15290"
     CDS             1945..2892
                     /gene="hemF"
                     /locus_tag="PKV56_15290"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HOX69223.1"
                     /translation="MTTQAVRDYLLGLQDRIISTMEREGGQPFRTDGWVRDPGERLQG
                     DGLSRLIEDGDLLERGGCNFSHVRGPQLPPSATQHRPELAGAPFEAMGVSLVFHPRNP
                     YVPTVHMNVRLFAALPKDAEPVVWFGGGMDLTPYYGFEEDAAHFHRVNRDALAAFGDD
                     KYPRFKTWCDEYFFLKHRNEPRGVGGVFFDDFAELGFEGSFSMIRSVGDAMIDAYLPI
                     VQRRKDTPYGERERDFQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPIVKW
                     RYDWKPDAGTPEARLYSDFLRPRDWADWSEAQRSAGTVA"
     gene            2889..3458
                     /gene="nadD"
                     /locus_tag="PKV56_15295"
     CDS             2889..3458
                     /gene="nadD"
                     /locus_tag="PKV56_15295"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOX69224.1"
                     /translation="MRRIGLFGGSFDPVHNAHLALARAAWRDLELDEVRWIPAGHPWQ
                     KARALTDAGHRAAMVALAVEAEPGFVLDPIELRRPGPSYTLDTVRELQAAQPDAQWFL
                     ILGQDQQAALPTWHGAEELLGRVTLAVARRPGSDDGGAPAPAARTVFLPPMDLSSTQV
                     RARCAAGLPIDEMVPAGVARYIDQHGLYR"
     gene            3515..4345
                     /gene="rsfS"
                     /locus_tag="PKV56_15300"
     CDS             3515..4345
                     /gene="rsfS"
                     /locus_tag="PKV56_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOX69225.1"
                     /translation="MDIKKLQRVIVDGLEDVKGQNILVFDTEHLSSLFERVIIASGTS
                     NRQTKSLAASVRDAVKKAGYPVPRVEGEVNGEWIIVDCGAAVVHLMQPNIREYYHLEE
                     IWGDKPVKLKLDSAPKRLVKAEEVPAAAPAKKAPARKAAASSKPAAAPPAKKAPARKS
                     TAAHAAAAEAEAPAPKRRTTSAKPAAPAKRAAPAKTGVTRTVGVKTAAASPARKAPAR
                     KAPSSPAPRVARKAPIKTIAVNKPTTKKAATARAASSSAAPKRASASKPAAKKAPRGA
                     "
     gene            4342..4809
                     /gene="rlmH"
                     /locus_tag="PKV56_15305"
     CDS             4342..4809
                     /gene="rlmH"
                     /locus_tag="PKV56_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOX69226.1"
                     /translation="MKLLLVAVGQRLPAWADEAYQDYAKRFPPDLRLELKAVKAEPRS
                     GKTADQLMAAEATRIEAALPRGVRRVVLDEHGSRLTTMQLAQRLTAWVNDGRDAALIV
                     GGPDGLAPPLKDSADETLRLSDLTLPHAFVRVLLAEALYRAWTVTINHPYHRE"
     gene            4806..5459
                     /locus_tag="PKV56_15310"
     CDS             4806..5459
                     /locus_tag="PKV56_15310"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOX69227.1"
                     /translation="MKSAQPITPIESVAAKPSHDFVYLASQSPRRRQLLEQIGVRFEL
                     LLPDPSEDAESLEAERPGELPLAYVQRVTLAKLGAARARLIRLGLPPAPVLCSDTTVA
                     LGRRIYGKPLDATDAAATLRKLSGRTHRVITAVAVALGDTEALAVSTSRVSFAELDEA
                     TIDRYVAGGEPFGKAGAYAIQGAAGAWVTRIDGSYTGIMGLPLHETAELLRAIGVRT"
     gene            5505..7004
                     /gene="rng"
                     /locus_tag="PKV56_15315"
     CDS             5505..7004
                     /gene="rng"
                     /locus_tag="PKV56_15315"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HOX69228.1"
                     /translation="MQDILINWTPQETRVAVIEHGAVQDLYIERTLERGLVGNIYAGR
                     VVRVLPGMQSAFIDIGLERAAFLHVADVQLAADQIRQNASAAGGGPVAPPPPIERLVF
                     EGQTMTVQVIKDPIGTKGARLSTQISIAGRLLVFLPQDDHIGISQKIGSHELREQLRA
                     RVSQLVQLPEGSGPTGGFILRTNAEESTDAELADDITYLRKTWSAIRERSFKLPPGSL
                     LHQDLSLVERVLRDLAHEGTQSIRIDSNQQYDVLKAFGEAFTPTSASKLDLYKGERPI
                     FDLFSIDEDIARALARRVDLKSGGYLIIDQTEALTTVDVNTGGYVGARNFDDTIFKTN
                     LEATQAIARQLRLRNLGGIIIVDFIDMTREEHQAAVLAEFKKQLVRDRTKVTVSGFTQ
                     LGLVELTRKRTRESLSHMLCEPCPTCEGKGQVKTARSVCYDILREILREAKQFNPKEF
                     RIIASPSVVEMLLDEESQHLAGLSDFIGKPISLRAENAGSPEHYDIVLM"
     gene            7068..>7831
                     /gene="asd"
                     /locus_tag="PKV56_15320"
     CDS             7068..>7831
                     /gene="asd"
                     /locus_tag="PKV56_15320"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746858.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOX69229.1"
                     /translation="MSERMAVLPQYLLPKKLMTEFAGWCAASRSRAWTSRVIPWFIAR
                     YGVDMSEAAEPDAAAYACFNDFFTRALRDGARPLAEADFVCPVDGAVSQCGAIEGDRI
                     LQAKGHSYSVQAMVGGDARLAALFEQGEFATVYLSPKDYHRIHMPCDGRLVRMIHVPG
                     DLFSVNPTTARGVPGLFARNERVVCVFESPSHGTFVMALVGATIVGSMATVWHGLVNP
                     PRPGVVREWHYDDRSILMRQGEEMGRFLLGSTVVLL"
BASE COUNT         1342 a         2614 c         2664 g         1211 t
ORIGIN      
        1 acatgccggc cgacacggtc aagaagaaca tcgacaaggc caccggcaac ctcgagggcg
       61 tgcactacga cgagattcgc tacgagggct acggcatcgg cggcgcggcg gtgatcatcg
      121 acacgatgac cgacaacaag gtgcgcacgg tggccgaggt gcgccacgtg ttcagcaagt
      181 acggcggcaa cctcggcacg gacggttcgg tctcgttcca gttcaagcat tgcggtcaga
      241 tcgtttttgc gccggggacc agcgaggaca cggtgatgga gatcgcgctc gaggccggtg
      301 ccgaggacgt ggtcaccgac gacgaaggtg cgatcgaggt gatgacctcg ccgcatgatt
      361 tcgaggccgt gaaagcggcg ctcgaggcag ccgggctgaa gcccgaggtg gccgaagtga
      421 cgatgcgtcc ggagaacacc gtcgaactgg cgggcgagga cgcgctgcgc atgcagaagt
      481 tgctcgacgt gatcgaggac ctggacgacg tgcaggcggt gttccacaac gccgagctgt
      541 ccgaatgagc ccccacgctc gccttcgctc actgcccccc gcgggggcgc ccggcctcct
      601 tgaggcggct cggcggagtc cggtatgaaa gtcctcgtga tcggctccgg cgggcgcgaa
      661 cacgcgatcg cctggaagct cctgcaatcg gcccgggtgc agaccgtcta cgtcgcgccg
      721 ggcaacggcg gcaccggcag cgacaaccgg ctgcacaacg tgccgctgac ggatttcaag
      781 gatctggccg agttcgcgga gcgcgagaag atcgcgctga cggtcgtcgg ccccgagggt
      841 ccgctggcgg gcggcatcgt cgattttttc cgggcgcgcg ggctgcgcat cttcggcccg
      901 acgcaggccg ccgcgcagct cgaaagctcg aaggcctacg ccaaggcgtt catgaagcgc
      961 caccggattc cgaccgcgtc gtacgagacc ttcaccgagt cggcggcggc gcatgactat
     1021 gtggacacgc acggcgcgcc gatcgtcatc aaggccgatg gtctggccgc gggcaagggc
     1081 gtggtcgtgg cgaccacgat ggcgcaggcg cacgaggcga tcgactggat gctcggcacc
     1141 gacggggccg ccaacaccct cggcgtgcag cacaacgccg gggccgacgg ccaggcggtg
     1201 ccccgggtcg tgatcgaatc gttcctcgaa ggcgaggagg ccagtttcat cgtcatgtgc
     1261 gacggcaaga acgtcgtgtc gctggcgacc agccaggatc acaagcggct gctcgatggc
     1321 gatgaaggcc ccaacaccgg cggcatgggg gcctactctc cggcgccggt ggtcacgccc
     1381 aacgtgcacg cgaaggccat gcacgagatc atcctgccga ccatccaggg catggcgaag
     1441 gacggcattc cgttcaccgg tttcctgtac gccggactga tgatcgatgc gcagggccag
     1501 ccacgcaccg tcgagttcaa ttgccggctc ggagatcccg agacgcagcc gatcctgatg
     1561 cggctcaaga gcgacctgtt cgatgtgttc atgcacgcca ccgacggctc gctcgacgaa
     1621 gtcgaactgc aatgggaccg ccgtgtcgcg ctcggcgtcg tcgtcgcggc acacggctac
     1681 ccgtacacgc cgcgcaaggg cgatgcgatc cacggcattc cggccgaagc ggccgacggc
     1741 atcgtgttcc acgccgggac cgaactgcgc gacggccagt tggtgacttc gggggggcgc
     1801 gtgctgtgcg tgacggtgct ggccgattcg gtgaagcagg cccagccacg cgcctatgag
     1861 ctgttgcatg ccatccactt cgatggcatg cagttccgcc gagacatcgg ccatcgcgcg
     1921 gtcaagcgct aatccgcccc gatcatgact acacaagccg ttcgagacta cctgctgggc
     1981 ctgcaggacc gcatcatcag cacgatggaa cgcgagggtg gccaaccttt ccgcaccgac
     2041 ggctgggtgc gggatcccgg cgagcggctg cagggcgatg gcttgtcccg gctgatcgag
     2101 gatggcgacc tgctcgaacg cggcggctgc aatttctcgc acgtgcgcgg tccgcagctg
     2161 ccgccatccg ccacgcagca ccggcccgaa ctcgccggcg cgccgttcga ggcgatgggt
     2221 gtgtcgctgg tgttccaccc ccgcaacccg tatgtgccca cggtacacat gaacgtgcgc
     2281 ctgttcgccg cattgccgaa ggatgccgag ccggtggtct ggttcggcgg cggcatggac
     2341 ctgacgccct attacggctt cgaagaggat gccgcgcact tccaccgtgt caaccgcgac
     2401 gcgctcgcgg ccttcggcga cgacaagtac ccgcgcttca agacctggtg cgacgaatac
     2461 ttcttcctca agcatcgcaa tgaaccgcgc ggcgtcggtg gcgtgttctt cgacgacttt
     2521 gccgaacttg gcttcgaggg cagtttctcg atgatccggt cggtgggtga cgcgatgatc
     2581 gatgcgtacc tgccgatcgt gcagcggcgc aaggacacgc cgtacggcga gcgcgagcgc
     2641 gatttccagg cctaccgccg cggccgttat gtcgagttca acctggtgtt cgaccgcggc
     2701 acgctgttcg gcttgcagtc gggtggtcgc accgagagca tcctgatgtc gatgccgccg
     2761 atcgtgaagt ggcgctacga ctggaagccc gatgccggca cgcccgaagc gcgcctgtac
     2821 agcgacttcc tgcggccgcg cgactgggcc gactggagcg aagcgcagcg tagcgcgggc
     2881 accgtggctt gaggcgtatc ggcctgttcg gcggcagctt cgatccggtg cacaacgcac
     2941 accttgccct cgcgcgggca gcgtggcgcg acctcgaact cgacgaagtg cgctggattc
     3001 cggccggaca tccctggcag aaggcgcgtg cattgaccga cgccgggcat cgagcggcga
     3061 tggtggcgct tgccgtcgag gccgagccgg ggttcgtgct cgatccgatc gaactgcggc
     3121 ggcccggtcc gagctacacg ctcgacacgg tgcgcgaact gcaggcagcc cagcccgatg
     3181 cgcagtggtt cctgatcctc gggcaggacc agcaggccgc tttgccgacc tggcatggcg
     3241 ccgaagagtt gctcggccgc gtcacgctgg cggtggctcg gcggcctggc tctgacgatg
     3301 gcggcgcacc ggcgccagct gcacgcaccg tcttcctgcc accgatggat ctgtcgtcga
     3361 cgcaggtgag ggcgcgctgc gcggccggcc tgccgatcga cgaaatggtg ccggccgggg
     3421 tggcgcgcta tattgaccaa cacggtctgt accggtgatg ccaccgggac agcctcattt
     3481 caccgcaagc gtcgaggggc gcaccaggag ctgaatggac atcaagaaac tgcaacgcgt
     3541 catcgtcgat ggactggaag acgtcaaggg ccagaacatc ctcgtgttcg ataccgaaca
     3601 cctgtcgtcg cttttcgagc gcgtgatcat cgcctcgggt acaagcaacc ggcagacgaa
     3661 gtcgctggcc gccagcgtgc gcgatgcggt caagaaggca ggctatccgg tgccccgcgt
     3721 cgaaggcgag gtcaacggtg aatggatcat cgtcgactgc ggtgcagcgg tcgtgcacct
     3781 gatgcagccc aacatccgcg agtactacca cctcgaggag atctggggcg acaagccggt
     3841 caagctcaag ctcgacagcg cgccgaagcg gctggtgaaa gccgaggaag tgccggcagc
     3901 ggcgccggca aagaaggcac ctgcgcgcaa ggcggctgca tcgagcaagc cggcagcggc
     3961 tcctcctgcc aagaaggctc cagcgcgcaa gtcgaccgct gctcacgccg cggcagccga
     4021 ggccgaagcc ccggcaccga agcgtcgcac gacgtccgcg aagccggctg ctccggcgaa
     4081 gcgtgccgca cccgccaaga ccggggtgac gcgcacggtc ggcgtcaaga cggcggcggc
     4141 gagcccggcc aggaaagctc ccgctcgcaa ggccccgtcg tcgcctgctc cgcgcgttgc
     4201 ccgcaaggcg ccgatcaaga ccatcgcggt caacaagccg acgacgaaga aggctgcgac
     4261 cgcccgggcc gcgtcctcgt ccgccgcacc gaagcgggcg tccgccagca agccggcagc
     4321 gaagaaggcc ccgcgcggcg cgtgaagctg ctgctggttg ccgtcggcca gcgcctgccc
     4381 gcgtgggccg acgaggccta ccaggactac gcgaagcgct ttccgcccga cctgcgcctg
     4441 gaattgaaag cggtgaaggc cgaaccgcgc agcggcaaga cggccgatca gctgatggcc
     4501 gccgaggcca cgcgcatcga ggcggcgctg cccaggggcg tgcgccgtgt cgtgctcgat
     4561 gagcatggtt cgcgtctgac gacgatgcag ctggcgcagc gcctgaccgc ctgggtgaac
     4621 gacgggcgcg acgccgcgct gatcgtcggc gggcccgatg ggctggcgcc gccgctgaag
     4681 gacagcgccg acgaaacgct gcgcctgtcc gacttgacgc tgccgcacgc gttcgtgcgg
     4741 gtgttgctgg ccgaggcgct ctaccgggcc tggacggtga cgatcaacca cccctaccac
     4801 cgcgaatgaa gtccgcgcaa ccgatcacgc cgatcgaatc cgtggcggcg aagccgagtc
     4861 acgacttcgt ctaccttgca tcgcagagcc cgcgccgccg ccagttgctc gagcagatcg
     4921 gcgtgcgctt cgaactgctg ctgcccgacc cgtcggagga cgccgagtcg ctcgaagccg
     4981 agcgcccggg tgagctgcca ctggcctacg tgcagcgcgt gacgctcgcg aagctcgggg
     5041 cggctcgtgc gcgcctgatc cggctgggct tgccgcccgc gcccgtcctt tgctcggaca
     5101 ccacggtggc gctcgggcgg cgcatctacg gcaagccgct cgatgcgacc gatgcggcgg
     5161 cgacgctgcg aaagctgagc ggtcgcacgc accgcgtcat cacggcggtg gcggtggcgc
     5221 tgggcgacac cgaggcgctg gcggtcagca cgtctcgcgt gagcttcgcg gaactcgacg
     5281 aggcgaccat cgatcgctat gtggctggtg gcgaaccgtt cggcaaggcc ggtgcctacg
     5341 cgatccaggg cgcggccggg gcatgggtga cccgcatcga cggcagctac accggcatca
     5401 tgggcctgcc gctgcacgag acggccgagt tgctgcgtgc catcggggta cgcacctgaa
     5461 tcggcccaca agctgaggac gcgccctcgc ataccatcga cccaatgcaa gacatcctga
     5521 tcaactggac cccgcaggag acccgtgtcg cggtgatcga gcacggtgcg gtccaggacc
     5581 tttacatcga gcgcacgctg gagcgcggtc tggtcggcaa catctatgcc ggtcgcgtcg
     5641 ttcgcgtgct gccaggcatg cagtcggcct tcatcgacat cgggctcgag cgggctgcct
     5701 tcctgcatgt ggccgacgtg cagctcgcag ccgatcagat ccggcagaac gccagcgcag
     5761 ccggtggcgg cccggttgcg ccgccgccgc cgatcgagcg cctggtcttc gaaggccaga
     5821 cgatgaccgt gcaggtcatc aaggacccga tcggaaccaa gggggcgcgc ctgtccacgc
     5881 agatcagcat cgccggccgc ctgttggtgt tcctgcctca ggacgatcac atcggcatca
     5941 gccagaagat cggctcgcac gaactgcgcg agcagctgcg cgcgcgggtg agccagctcg
     6001 tgcaactgcc cgaggggagc ggtccgaccg gcggcttcat cctgcgcacc aacgccgagg
     6061 aatcgaccga cgccgaactg gccgacgaca tcacctacct gcgcaagacc tggtcggcga
     6121 tccgcgagcg cagcttcaag ctgccccccg gcagcctgct acatcaggat ctgagtctgg
     6181 tcgagcgcgt gctgcgcgat ctcgcgcacg agggaacgca gtcgatccgc atcgattcga
     6241 accagcagta cgacgtcctg aaggcctttg gcgaggcctt cacgccgacg tccgccagca
     6301 agctcgatct gtacaagggc gagcggccga tcttcgacct gttcagcatc gacgaggaca
     6361 tcgcacgcgc gctggcccgg cgcgtcgacc tgaagtccgg cggttatctg atcatcgacc
     6421 agacggaggc gttgaccacc gtcgacgtca acacgggcgg ctatgtcggc gcgcgcaatt
     6481 tcgacgacac gatcttcaag accaatctcg aggcgacgca ggccatagct cgccagctgc
     6541 ggctgcgcaa cctcggcgga atcatcattg tcgacttcat cgacatgacc cgggaagagc
     6601 accaggctgc cgtgctggcc gagttcaaga agcaactggt gcgcgaccgc accaaggtca
     6661 cggtcagcgg cttcacgcaa ctgggactgg tcgagctgac ccgcaagcgc acgcgcgagt
     6721 cgctgtcaca catgctttgc gagccctgcc cgacttgcga aggcaagggt caggtgaaga
     6781 ccgctcgcag cgtctgctac gacatcctgc gcgagatcct gcgcgaggcg aagcagttca
     6841 accccaagga gttccgcatc atcgccagcc cgtcggtcgt cgagatgctg ctcgacgagg
     6901 agagccagca tctggccggc ctcagcgatt tcatcggcaa gccgatctcg ctgcgcgccg
     6961 agaacgcggg gtcgcccgag cactacgaca tcgtactgat gtgatgcggc gcgccttgcg
     7021 cttgcttgaa ccgatggaat tcgccgggag tcgcctcgtt gcctgatctg tccgaacgca
     7081 tggcggtgct gccgcagtac ctgctgccca agaagttgat gaccgagttc gcgggctggt
     7141 gcgccgcttc gcgctcgcgt gcctggacga gtcgtgtcat cccctggttc attgcccgct
     7201 acggcgtgga catgtccgaa gcggccgagc cggacgcagc cgcctacgcc tgcttcaacg
     7261 atttcttcac gcgtgcgctg cgcgatggcg cgcggccgct ggccgaggcc gacttcgtct
     7321 gcccggtgga cggtgcggtc agtcagtgcg gcgccatcga aggggaccgc atcctgcagg
     7381 ccaagggcca cagctacagc gtgcaggcga tggtgggcgg cgatgcccgc ctcgcggcgc
     7441 tgttcgaaca gggcgagttc gccaccgttt acctgagccc gaaggattac caccgcatcc
     7501 acatgccgtg cgacgggcgc ctcgtgcgca tgatccatgt gccgggcgat ctgttttccg
     7561 tcaacccgac cacggcgcgt ggcgtgcccg ggctgttcgc ccgcaacgag cgcgtggtct
     7621 gtgtcttcga gtcgccgtcg catggcacct tcgtgatggc gctggtcggg gccaccatcg
     7681 tgggcagcat ggcgaccgtc tggcacggcc tggtgaaccc gccccgaccc ggcgtcgtgc
     7741 gcgagtggca ctacgacgac cggtccatcc tgatgcgtca gggcgaagag atgggccgct
     7801 tcctgctcgg ctcgaccgtg gtcctgctgt t
//
LOCUS       DAOORV010000152         7824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11484_length_7824_cov_7.027288, whole genome shotgun sequence.
ACCESSION   DAOORV010000152 DAOORV010000000
VERSION     DAOORV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11484_length_7824_cov_7.027288"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="PKV56_15325"
     CDS             complement(<1..2321)
                     /locus_tag="PKV56_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOX69230.1"
                     /translation="MSSGIHRPSTGLIRIRGARQHNLKNLDLDIRTGEMTVVTGPSGS
                     GKSSLVFDTLYAEGQRRYVETFSAYARQFLDRMDRPAVDRVDGVPPAIAIDQTNPVRT
                     SRSTVGTMTELNDHLKLLYARAADLFDRQTAQRVRHDTPETIYAELMARTVVADPRLV
                     LTFPVELPAVSTPEEVEQWLSASGFTRVQAEREIATPTGPRKLLDVVADRFRIQGTEK
                     VRAIEAIEMALKRGGGRVNVYALVDESQPAIWRFSTGLHCPDSDLRYADPQPALFSFN
                     SAMGACDTCRGFGRVIGVDYDLVIPDHRKTLRSGAIKTLQTPAWKDAHEDMLKYAGEA
                     GIPRDTPWSQLNEAQRDFIIGGSPQWKGNWNKQWYGVKRFFEYLESKAYKMHIRVLLS
                     KYRSYTTCGTCSGARLKTESLLWRLGTKADADEVMAPSLRSMPHGVGWSREQLEALPG
                     LTVHDLMLLPIERIRRLFDGLSLPSTLLDDALKLLVDEIRTRLKYLCDVGLGYLTLDR
                     QSRTLSGGEVQRINLTTALGTSLVNTMFVLDEPSIGLHPRDMNRIIEAMHRLRDAGNT
                     LVVVEHDPAVMLAADRLIDMGPGPGERGGQIVFDGTPDTIRDADTLTGAYLGGRKHIG
                     SGFIRLVTPATPRLILEGARDHNLRDVTVEFPLQCLVTVTGVSGSGKSTLIQDVLYPA
                     LARHFGKSSDAPGAHDRLLGADWLSEAVFVDQSPIGKTARSNPASYVGAFDEIRKLFA
                     ASPLAQQRGYGAGMFSFNAGDGRCPTCGGSGFE"
     gene            2524..4992
                     /locus_tag="PKV56_15330"
     CDS             2524..4992
                     /locus_tag="PKV56_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX69231.1"
                     /translation="MAESPAVSPVVASLPAALSARREPSCADASDRALRQVMASDGVH
                     WELHLTTGQWWCSPALPHPLGLAEGAPTAASPFERCIPEDRERIDDACAEAIQQRGGF
                     TLDLRLLDDDGHHRWWRCTARVLPASNGTPGYLAGVLREVHAERQTMQRLEAVAARFN
                     RAMEASSEAHFERTAGLDDFFVSDRICTLLGYPSGTPAPAREVYFSWVHPDDRAALHA
                     QIVTAGAGPGPWESVYRLRHRDGTFRWFRARGRSEFDPEGRLRMTGMLSDTHEQTLVR
                     RDIEQQRQHLEAMVEERTVRLETALAEAQRQREQAERANESKSTFLAHMSHEIRTPLN
                     GVLGLNELALREATGTQQKRYLKLALQSGRNLLGIINDVLDFSRLSAGALPSAEQPFD
                     LCEVLTQAMRAIMPAVRTKGLGLIYDHVGGVSRVVGDPQRLQQIMTNLLSNAVKYTEQ
                     GHIELVTHMHEVTAGQCMARIVVRDTGCGMSAEVASRAFQPFVQGDDSLARRHGGTGL
                     GLSIALGLAQSVGGSLTLDTAPDRGSTFTLELPLGVQPGEQPATGRPPPGTAWLVHTR
                     ALPAQATQRRLQRMGWQCDVLGSMQELMARADAAGVAPDLVLLGESALEPDADLCRLR
                     GLLPHTNIVLLARPDWNDPPLEAAARAAQMPLVFTPLSPAALFDLLCMQGPQSDRIVS
                     GFSTLAGLPAPVEGNADSDVLVVEDNMVNQVIVCEMIDALGLRTRLAEDGGTAVEACR
                     ARPPALVLMDLQLPGVDGLEATRQLRLLQQRGTLPNFPIIALTAHATPQDRDSCLAAG
                     MVGYLTKPVAMADLRNELSRWLRC"
     gene            5129..6685
                     /locus_tag="PKV56_15335"
     CDS             5129..6685
                     /locus_tag="PKV56_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HOX69232.1"
                     /translation="MNDNLYCALRDAFPKDIDRTAIETADGERLFYTWKDLDRSSAMI
                     ANLLQSLDLPAGSRVLVQTDKSVEALILYLAVLRAGLVYVPLNNAYQASELTYFIDNA
                     EPAVVVCASRDFGWLSKIAFLAGTRHVFTLDDNRSGSLLQRAAHQGDQQQPVPRAATD
                     VATIIYTSGTTGRSKGAMLSHGNLLSNARVLKSLWGWRTEAEGGDVLVHALPLFHVHG
                     LFVASHGALLNGSKMLWFAKFDPRSVTERLPEATVFMGVPTLYVRLLAEPTFGAGACR
                     NMRLFISGSAPLLAETFRQFSDRTGHTILERYGMSETVMLTSNPCKNAPDAPLLGRIA
                     GTVGFPLPGVDLRISGENGSRCAPGEIGAVEVRGPGIFQGYWRMPEKTSEEFTADGWF
                     KTGDVGHVDMHGRLTLIGRSKDLIISGGYNVYPAEIEGYLNELPGVDESAVIGVPHPD
                     FGEAVVAVVVPRAGATVDPQACIASLKQKIAGFKVPKRVSLADQLPRNAMGKVQKALL
                     REQHRSAFTG"
     gene            complement(6781..6942)
                     /locus_tag="PKV56_15340"
     CDS             complement(6781..6942)
                     /locus_tag="PKV56_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69233.1"
                     /translation="MWKLAIGFVLFAALAIFVLMKGGDIDMSGEKHGIDASHDAPAAA
                     SAPAPAASQ"
     gene            complement(6960..>7824)
                     /locus_tag="PKV56_15345"
     CDS             complement(6960..>7824)
                     /locus_tag="PKV56_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69234.1"
                     /translation="GTLSDKIGRKPIIMAGCLIAALTYFPLFTALTSAANPDLAKAQA
                     SSKVVVTADPSECSFQFNPTGTKKFTSSCDIVKQKLASASVSYDSIDAPAGTPATIAV
                     GETVITSKVTPEDIAAAKTAAETKLADLKAAPTPDATAIAAAETNLKNLADEKKAPDA
                     VLTAKLSGALKAAGYPAKADPAKVDKVKVVAILTFLVLLVTMVYGPIAAMLVELFPTR
                     IRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIVAGVTFVIGMLFVK
                     ETKDVNIYADD"
BASE COUNT         1314 a         2572 c         2610 g         1328 t
ORIGIN      
        1 tgctcgaagc ccgagccgcc gcaggtcggg cagcgcccgt cgccggcgtt gaagctgaac
       61 atgccggcgc cgtacccgcg ctgctgcgcc agcggcgacg cggcgaacag cttgcggatc
      121 tcatcgaacg cgccgacgta gctggccgga ttcgagcgcg cggtcttgcc gatcggcgac
      181 tggtcgacga acaccgcttc actcagccag tcggcaccga gcagccggtc atgcgcaccg
      241 ggcgcgtcgc tggacttgcc gaaatgacgc gccagcgccg ggtacaggac gtcctggatc
      301 agcgtcgact tgcccgatcc cgagactccg gtgacggtga ccaggcactg cagcgggaac
      361 tcgacggtca cgtcgcgcag gttgtggtcg cgcgcccctt cgaggatcag cctgggcgtc
      421 gcaggagtca ccagtcggat gaaaccgctg ccgatgtgct tgcgtccgcc gaggtaggcg
      481 ccggtcagcg tgtcggcatc gcggatcgtg tcgggcgtgc cgtcgaagac gatctgtcca
      541 ccccgctcgc cgggcccggg gcccatgtcg atcagccggt cggcggccag catcaccgcc
      601 ggatcgtgct cgaccacgac cagcgtgttg cccgcatcgc gcagccggtg catcgcctcg
      661 atgatgcggt tcatgtcgcg cgggtgcagg ccgatgctgg gctcgtccag cacgaacatc
      721 gtgttgacca gcgaggtgcc gagcgccgtc gtcaaattga tgcgctgcac ctcgccgccg
      781 ctcagcgtgc ggctctgacg gtcgagcgtc aggtagccga ggcccacatc gcacaggtac
      841 ttcagacgcg tgcggatctc gtcgaccagc agcttcagcg cgtcgtcgag cagcgtgctc
      901 ggcaacgaca ggccgtcgaa gaggcggcgg atgcgctcga tgggcaacag catcaggtca
      961 tgcacggtca ggcctggcag cgcttcgagt tgctcgcgcg accagcccac gccatgcggc
     1021 atggaacgca gcgatggcgc catcacctca tcggcgtcgg ccttcgtccc gagccgccac
     1081 agcagcgatt cggtcttcag ccgagcgcca ctgcaggtgc cgcaggtcgt gtagctgcgg
     1141 tacttcgaca agagcacgcg gatgtgcatc ttgtaggcct tgctctccag gtactcgaag
     1201 aagcgcttga cgccatacca ctgcttgttc cagttgccct tccactgcgg cgagccgccg
     1261 atgatgaagt cgcgctgcgc ctcgttgagc tgcgaccaag gcgtgtctcg cggtatgccg
     1321 gcctcgccgg cgtacttcag catgtcctcg tgcgcgtcct tccaggccgg cgtctgcagg
     1381 gtcttgatgg cgcccgaacg cagcgtcttg cgatggtccg ggatcaccag gtcgtaatcg
     1441 acgccgatca cgcggccgaa gccccggcac gtatcgcacg cgcccatcgc cgagttgaag
     1501 ctgaacagcg caggctgcgg atcggcgtag cgcagatcgc tgtccgggca gtgcaggccg
     1561 gtcgagaagc gccagatcgc gggctgcgac tcatcgacca gtgcatagac gttgacacgt
     1621 ccaccgcccc gtttcaacgc catctcgatg gcctcgatgg cacgtacctt ctcggtgccc
     1681 tggatgcgga atcgatcggc aaccacatcg agcagcttgc gcggcccggt cggcgtcgcg
     1741 atctcgcgct cggcctgtac ccgggtgaag ccgctggccg acagccactg ctcgacttct
     1801 tccggtgtcg acacggcggg cagttcgaca gggaaggtca gcaccagccg cgggtcggct
     1861 acaacggtcc gggccatcag ctcggcgtag atcgtctccg gcgtgtcgtg ccgaacgcgc
     1921 tgtgccgtct gacggtcgaa caggtccgcc gctcgcgcat agagcagctt caggtgatcg
     1981 ttcagctcgg tcatcgtgcc gaccgtgctt cggctggtcc gcaccgggtt ggtctggtcg
     2041 atcgcgatcg cgggcggcac cccgtcgacc cggtcgacgg ccgggcggtc catccggtcg
     2101 aggaactggc gcgcataggc gctgaacgtc tcgacgtaac gccgctggcc ttcggcatac
     2161 agcgtgtcga acaccaggct ggatttcccg gaaccgctgg ggccggtgac cacggtcatc
     2221 tcgccggtgc ggatgtcgag gtcgaggttc ttcaggttgt gctgccgtgc accgcgaatc
     2281 cggatcagac ccgtcgaagg tcggtggata cctgaggaca tgcggcaatt cctggctgcg
     2341 aaagcagcga agccgaagat tctagaagtc gaatgaactc ccggtgggag ccagggatcg
     2401 atatcaaagg cggtgcttgt tacgcggttg tttcaactgc gcgcggattc cgtccgttcc
     2461 tgatggcaca acctacatat cagggtccac cctgagtccc gatcgatttc caggtatccc
     2521 tgcatggcag agtccccagc cgtttcgccc gttgtcgcaa gcctgcccgc cgccctttcg
     2581 gcccggcgag agccaagctg cgccgacgcg tcggaccggg cgctgcggca agtgatggcc
     2641 agcgacggcg tgcactggga actgcacctg accaccggcc agtggtggtg ctcgccggcc
     2701 ttgcctcatc cgctggggct ggccgagggt gcgcccacgg cagcttcgcc attcgaacgc
     2761 tgcattcccg aagaccgcga gcgcatcgac gacgcctgtg ccgaggcgat acaacagcgt
     2821 ggcggcttca cgctcgatct ccggctgctc gacgacgatg gtcaccaccg ctggtggcgg
     2881 tgcacggcac gcgtgctgcc cgcatccaat ggcacgccgg ggtacctggc cggtgtcctg
     2941 cgcgaggtgc atgccgaaag gcagaccatg cagcgcctcg aagccgtggc ggctcgcttc
     3001 aaccgcgcca tggaggcgtc ctccgaggcg cacttcgaac gcaccgccgg cctggatgat
     3061 ttcttcgtca gtgaccgaat ctgcaccctg ctcggctacc cgagcggcac gccggcaccg
     3121 gcgcgcgagg tttacttcag ctgggtgcat ccggacgacc gcgctgcact gcacgcgcag
     3181 atcgtcaccg cgggcgccgg gcccggtccg tgggagtcgg tataccggct gcgtcaccgc
     3241 gatggcacct tccggtggtt ccgggcacgc ggacgctcgg aattcgaccc ggaaggtcgt
     3301 ctgcggatga ccggcatgct cagcgacacg cacgagcaga cactggttcg ccgcgacatc
     3361 gagcagcagc gccagcacct ggaagcgatg gtcgaggagc gcacggtgcg gctcgagacc
     3421 gcgcttgccg aagcccagcg ccagcgcgaa caggccgagc gggccaatga atcgaaatcg
     3481 acgttcctgg cacacatgag ccacgagatc cgtaccccgt tgaatggggt gctgggtctc
     3541 aacgaactgg cgctgcgcga agccaccggc acgcagcaga agcgttacct gaagctggcg
     3601 ctgcagtcgg gacgcaacct gctcggcatc atcaacgacg tgctcgattt ctcgcgcctg
     3661 tcggccggcg cactgccctc tgcggagcag cccttcgacc tctgcgaggt gctgacgcag
     3721 gccatgcggg cgatcatgcc ggccgtgaga accaaggggc tggggttgat ctacgaccac
     3781 gtgggcggcg tcagtcgcgt ggtcggcgac ccccaacgat tgcagcagat catgaccaac
     3841 ctgctgtcga acgccgtgaa gtacaccgag cagggacaca tcgaactcgt cacccacatg
     3901 cacgaggtga ctgcgggcca gtgcatggcg cgcatcgtcg ttcgtgacac cggctgcggc
     3961 atgagcgccg aggtcgcctc ccgggctttc cagcccttcg tgcagggcga cgacagcctg
     4021 gcccgccgcc atggcggcac cgggctcggc ctgtcgattg cgctcggcct ggcgcaatcg
     4081 gttggcggat cgctgaccct ggataccgcg ccggaccggg gatcgacctt cacgctcgaa
     4141 ctgccgctcg gagtgcagcc gggcgagcaa cccgcaacgg gccgccctcc gccgggaacc
     4201 gcgtggctgg tgcacacccg cgcactgccc gcgcaagcga cacagcggcg gctgcaacga
     4261 atgggttggc agtgcgatgt gctcggcagc atgcaggaac tgatggcccg ggccgatgct
     4321 gcaggtgtgg cgccggatct ggtcctgctc ggcgagtcgg ctctcgagcc cgatgcagac
     4381 ctgtgccggc tgcgcggcct gctgccgcac accaacatcg tgttgctcgc tcgcccggac
     4441 tggaatgacc cccccctcga ggcggccgca agggcggcgc agatgccgct ggtcttcacg
     4501 cctctgagcc cggctgcgct gttcgatctg ctgtgcatgc agggcccgca gtccgatcgc
     4561 atcgtctcgg gcttcagcac actggccggc ctgccggccc cggtcgaggg caacgccgat
     4621 tcggatgtgc tggtggtcga ggacaacatg gtcaatcagg tgatcgtgtg cgaaatgatc
     4681 gacgcgctgg ggctgcgcac ccgtcttgcc gaggacggcg gcaccgcggt cgaagcgtgc
     4741 cgggcgcgtc cgccggccct ggtgctgatg gatctccagt tgccaggcgt cgacgggctc
     4801 gaagccacgc ggcaactgcg cctgctgcag cagcgaggca cgctgccgaa ctttccgatc
     4861 attgcgctga cagcgcacgc gacgccgcag gatcgcgaca gctgcctggc cgcaggcatg
     4921 gtcggttacc tgaccaagcc ggtagcgatg gcagatctgc gcaacgagtt gtcgcgctgg
     4981 ttgcggtgct gagcacccca cgctgagcgg gctcgttgcc ctccgaggca gcccgccggg
     5041 gcggaatgcc ggggcagccc accggcagca ggtgaccctc gctgcgcgaa aatcgtcgcg
     5101 attgattacg cagaccttca gcgacgacat gaacgacaac ctctattgcg cactgcgcga
     5161 cgcattcccc aaggacatcg accgaaccgc catcgaaacc gccgacggcg aaaggctgtt
     5221 ctacacgtgg aaggatctgg atcgcagcag cgccatgatc gcgaacctgc tgcagtcgct
     5281 ggacctgcct gccgggtccc gggtgctggt tcagaccgac aagtcggtcg aggcgctgat
     5341 cctctacctg gccgtgctgc gcgccggact ggtctatgtg ccgctgaaca acgcctatca
     5401 ggccagcgaa ctcacctact tcatcgacaa cgccgaacct gccgtggtgg tctgcgcatc
     5461 gcgtgatttc ggctggctct cgaagatcgc tttcctcgcc ggtacgcggc acgtcttcac
     5521 gctcgacgac aaccgcagcg gaagcctgct tcagcgcgcc gcccaccagg gcgaccagca
     5581 gcaaccggtg ccgcgcgcgg ccaccgatgt cgccaccatc atctacacca gcggaaccac
     5641 ggggagaagc aagggcgcca tgctgagcca cggcaatctg ctcagcaatg cacgggtgct
     5701 gaagtcactg tggggctggc gcacggaggc cgaaggcggc gatgtgcttg ttcacgcgct
     5761 gccgctgttc catgtgcacg ggctgttcgt cgcgtctcat ggggctctac tcaacggcag
     5821 caagatgctg tggttcgcga agttcgaccc gcgctccgtg acggagcgtc tgcccgaggc
     5881 gaccgtgttc atgggcgtgc cgacgctgta cgttcggctg ctggccgagc cgacattcgg
     5941 ggcgggggcc tgcaggaaca tgcggctctt catcagcggc tcggcaccgt tgctggcgga
     6001 aacgttccgg cagttcagtg accgcaccgg ccacaccatc ctcgagcgct acggcatgag
     6061 cgagacggtg atgctgacgt ccaacccctg caagaacgcg cccgatgcgc ccctgctggg
     6121 acgcatcgca ggaaccgtgg ggttcccgct gcccggagtc gacctgcgca tttccggcga
     6181 gaacggctcg cgatgcgcgc cgggtgaaat cggtgccgtc gaagtgcgcg ggcccggcat
     6241 cttccagggc tactggcgca tgcctgagaa gacatcagag gaattcaccg ccgacggctg
     6301 gttcaagacc ggcgatgtgg gccacgtgga catgcacgga cggctgacgc tgatcggacg
     6361 cagcaaggac ctgatcatca gcggcggcta caacgtctac cccgcagaga tcgagggcta
     6421 cctgaatgag ctgccgggcg tcgacgagag tgcggtcatc ggcgtgccgc atcccgattt
     6481 cggcgaagcg gtcgttgcgg tggtggtgcc acgcgccggc gccacggtgg atccacaagc
     6541 ctgcatcgcg tccctgaagc agaagatcgc ggggttcaag gtgccaaagc gggtgtcgct
     6601 ggccgatcag ttgccgcgca atgcgatggg caaggtgcag aaggccttgc tgcgcgaaca
     6661 gcaccgcagc gcgttcaccg gctgactccg cgagacattg ccgggcaaag gaaaaagccg
     6721 gcgggtctcg cgacccgccg gcttcgtcat ttgaagggtc gtcgttcaga cgacccggat
     6781 tcactggctc gcggccgggg ctggcgccga ggcggcagcc ggtgcgtcat gggacgcatc
     6841 aatgccgtgc ttctcaccgc tcatgtcgat gtctccacct ttcatgagca cgaatatcgc
     6901 cagtgccgcg aacagcacga atcctatggc cagtttccac atgcttggaa tctcctgctt
     6961 cagtcgtcag cgtagatgtt gacgtccttg gtttccttga cgaacagcat gccgatcacg
     7021 aaggtgacac ccgcgacgat gatcgggtac cacaagccgt tgtacatgtt gcccgtctgc
     7081 gccacgatgg cgaaggccgt ggtcggcagc aagccaccga accagccgtt gccgatgtga
     7141 tacggcaggc tcatcgaggt gtagcggatg cgggtcggga acaactccac gagcatcgcg
     7201 gcgatcgggc catagaccat ggtcaccagg agcaccagga aggtcaggat ggcgacgacc
     7261 ttcaccttgt cgaccttggc tggatcggcc ttggccggat aaccggctgc cttcagcgcg
     7321 cctgacagct tggcggtgag gacggcgtcc ggagccttct tctcatccgc caggttcttc
     7381 aggttggtct ctgcagctgc gatggcagtg gcatccggcg tcggtgcggc tttcagatcg
     7441 gccagcttgg tctcggcagc ggtcttggcg gcagcgatgt cctcaggcgt gaccttggac
     7501 gtgatgaccg tctcgcccac cgcgatcgtg gctggcgtac cggctggtgc gtcgatgctg
     7561 tcgtagctca ccgaagccga ggccagcttc tgcttgacga tgtcgcagga actggtgaac
     7621 ttcttcgtgc ccgtcgggtt gaactggaac gagcactcgc tcgggtcggc ggtcaccacg
     7681 accttagacg aagcctgagc cttggcgagg tcggggttgg ccgcggaggt caacgcagtg
     7741 aacagcggga agtacgtcag cgcggcgatc aggcagccgg ccatgatgat gggcttgcgg
     7801 ccgatcttgt cggacagcgt gccg
//
LOCUS       DAOORV010000153         7795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11522_length_7795_cov_7.204522, whole genome shotgun sequence.
ACCESSION   DAOORV010000153 DAOORV010000000
VERSION     DAOORV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11522_length_7795_cov_7.204522"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3258
                     /locus_tag="PKV56_15350"
     CDS             <1..3258
                     /locus_tag="PKV56_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX69235.1"
                     /translation="VVEACAGAGKTWMLVSRILRALLDGAEPQEILAITFTRKAAGEM
                     RARLAEWLTTLSADVGTEAQRVEALQQRGIDAATAQQLAPALAGLQQQLQRAGRSVEI
                     RTFHAWFSQLLKAAPLDLLIELGVQRDAQIVEDIADHESEVFRRFHTAIAADEALRAD
                     FYHLVQRRGRSQLRKWLDSAWSKRVEIELADAAGTLESSVAVPSASWPGAQAGVHPAQ
                     WVRIPLARQTLQCCASALGAGGKAKQTTAARAIEAALSQETRSGDDSEVFDAFWAALF
                     TQKDTPRLGLGDGPEQRAAIELLVELRHAIAQHEAHEEHLQMVRLSRVLFAELATYKR
                     SRGLVEMADLERCAVALLGDSTLSAWVQERLDARVRHLLIDEFQDTSPLQWHALAAWL
                     SGYAGAGGGASGQRPPSVFIVGDPKQSIYRFRRAEPKVFEAARDFVKQGLEGQVLSCD
                     HTRRNAPEVLAVLNSVFEQAGRDGEFAGFRPHTTALEPVSGAGVCSLPLVLREARERD
                     DAADGQVWRDTLSVPRVEPEQRLRQQEADRVALAVRDVLAGGVKPGQVQVLCRKRESL
                     RLAAGALESLGVPCAAVEEAALFDAPEVRDLVAVLDVLASPSHALSLAQALKSPIFGC
                     SDDDLVQLATAVAASRGGGEVPRRPPDPLAWWSALHGVAEPSAALRRARALFGLWQQA
                     AAVLPPHDLLDRIVDEGEVMQRTLAAVPPTRRAAARAAIHALLAQSLMLDGARGATPY
                     NFVRALRRRRLKIAAPAQPDAVQLLTIHGAKGLEAEVVFIMDTQPEPNVADTATLLVQ
                     WPVESRHPVCCAFVYAESRCPPLLQTLFEAEVAARRREELNSLYVSMTRAKHRVVISA
                     TVPHHASKVSWWQRLEPYAPAWPSEPAVCAHADEPGADLSLPVLPAWDPPRLAAAVPA
                     ERVRDKGDECSAASRLGQAVHRVLEWAVRGELPGSGALDALGAAAAREFGAEPPAVQR
                     VAVAILRSAACQRFFDAAGLIWAGNEVSVTDGGEVLRIDRLVHLREAGPGVASQRASG
                     GVWWVLDYKLSEAPQELDLHRLQLQRYQRAVSRLQPQAIVKAAFITGRGAVVELD"
     gene            3414..5339
                     /locus_tag="PKV56_15355"
     CDS             3414..5339
                     /locus_tag="PKV56_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX69236.1"
                     /translation="MTGNPPSSASARPSQLGRNFGRFELLQLLGKSQGSMVWLVKDPH
                     LQQELVLTLPRVQPVDPAALERWLMPVRHAARLVHPNLAQGVEVAVQEHWPYAAVDRS
                     KGVTLGEWLALHPKPSPIEAVGWVCQVLEALAFAHEAGTAHHDLQLHSLLINDAGHVQ
                     LMALSTSSTQGTPAAADGVRRGVDQAVLVEADTLRAQREAAERDVLACGLLLHLLLTG
                     APVLDEPDISKAMLRLLPVGRDLVRLPWSTPHPIPEALRAIANRCTSSQDRQRYLSAR
                     TFLRALQGWLEAEALGRDGPLELMLDRLRTVGALPALPGMSARVERMAAMEGQHVEEI
                     ARLILQDMALSFELLREVNSVHVQGTQAAGSAPVLTIRRSIALIGLNGLRQAANGLRL
                     WPGPLSETGAQALRSLMDRVRLAGYTAQAMRPAGYDAEVVYLVTVLQNLGRLLAQYHF
                     PDEFEQIRHLMQGTPAPADAPPGTLGVPGWAESDASFAVLGIDIEALGAAVARHWGLG
                     EEMLHMIRRLPKDLPVRMPDNDSDVLRITASAANEAVDASLMPAAAPRSSAATAGVAA
                     DGSAMPSSAAKGSRGAGPRTSVAQRYARSLGLTPRIVQESLAAARQALRTGEPVQTSA
                     SRVEDAPAAEQAAVAAN"
     gene            5348..>7795
                     /locus_tag="PKV56_15360"
     CDS             5348..>7795
                     /locus_tag="PKV56_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOX69237.1"
                     /translation="MASLAAPPAVQRLGRFELRRLLGKGAQANVWLGFDTHLEREVAV
                     KIIRNGSGVAAGDASQWLQEARSVSRLTHPNIVPVFEADVHEGQPFLVFEYVAGHTLT
                     EVLRAKGAFAPQAAVGIVLDILSALRAAHETGIVHRDLKPSNVLIDIGGRARVMDFGI
                     AVKTNDTTQKNQIVGTPGYLSPEAAEGLSPTPAMDVFSAGLVLAELLAGKAVISEKDP
                     YRAIYRAAHEDLALPDSVGAEVDDALRAIVLRAMSRDPARRYVGAEAFGDALQAWMNP
                     VANESEAAATGAAATNGTLDFLLRRMRHKSDFPALSSAVSCIHRVANSETESLNSLAN
                     EILKDVALTNKLLRLVNTASYRHAGGGSISTVSRAAALIGFAGIRNMALSLVLLEHMQ
                     DKGHANLLKEEFLRSLMAGTLAGELCRTGRDSEEAFIGSMFQNLGRLLTQYYFPEEAR
                     QVRSLLSRAAASGDAPKPGAENSVAIQVLGLSFEDLGVGVAKSWGLPDSLQRCMRKPG
                     GEPPSRPPDAAAERLRWLAMAANDLTDTLMRSDAAASAGALADAAERYARVLGVSAKH
                     ICETTAVARIKLAESARAMNLVVGKNSLAARLMAPAPGTASSGLLDESTQPNTDSLSP
                     HELHATSLLCTSAADDAAAAPMGTPGRAAEVLAAGIQDITNSMVDSFKLNEVLRMILE
                     TMFRALGFRRVLFCLRDPKTETLTGRFGLGEGGDAAAALFKVPLKGGTDLFAAVCLKG
                     ADTLIRDATEAAMAERLPSWYRKTVNAPSFLLLPLMMKGSPFAMIYADQAQPGGIELD
                     EKELSLLRTLRNQAVMAF"
BASE COUNT         1235 a         2599 c         2651 g         1310 t
ORIGIN      
        1 gtcgtcgaag cctgcgcggg cgccggcaag acctggatgc tggtgtcacg catcctgcgc
       61 gcgctgctcg acggtgccga gccacaggag atccttgcga tcacgttcac gcgcaaggcg
      121 gccggcgaga tgcgggcccg gctcgccgag tggttgacca cgctgtcggc cgatgtcggc
      181 accgaggcgc agcgagtcga ggccttgcag cagcgcggaa tcgacgccgc caccgcgcag
      241 caactcgcgc cagcgcttgc cggcctgcag cagcaattgc agcgcgccgg ccgttcggtc
      301 gagatccgca ccttccacgc ctggttctcg cagttgctga aggccgcgcc gctcgatctg
      361 ctgatcgagc tgggcgtgca gcgcgacgca cagatcgtcg aggacatcgc cgatcacgag
      421 agcgaggtct tccgccgctt ccacactgcg attgcggccg acgaggcatt gcgagccgac
      481 ttctaccatc tcgtccagcg gcgcggtcga tcgcagttgc gcaagtggct ggattccgcg
      541 tggtccaagc gcgtcgagat cgagctcgcc gatgcggccg gcacgctcga gtccagcgtt
      601 gcggtgcctt ctgcctcctg gccgggggct caagccggcg tgcacccggc gcagtgggtg
      661 cggattccat tggcgcggca gacgttgcag tgttgtgctt cggcgctggg tgccggtggc
      721 aaggccaagc agacgacagc ggcgcgagcg atcgaggccg cgctgtctca ggagactcgg
      781 tctggcgatg actccgaggt gttcgacgcc ttctgggcgg ctcttttcac gcagaaggac
      841 acgccgcgtc tgggcctcgg cgacgggccc gagcaacggg ctgcgatcga gctgctggtc
      901 gaattgcgtc acgcgatcgc acagcacgaa gcgcacgaag agcatctgca gatggttcgt
      961 ctttcccgtg tcctgttcgc cgaactcgca acctacaagc ggtcgcgggg gctcgtcgag
     1021 atggcggatc tcgaacgctg tgcagtcgcg ctgctgggcg actcgacgtt gtcggcctgg
     1081 gtgcaggagc ggctcgatgc gcgcgttcgc catctcctga tcgacgagtt ccaggacacc
     1141 agcccgctgc agtggcatgc gcttgcggcg tggctgtccg ggtacgcggg cgcaggcggt
     1201 ggtgccagcg gccagcgccc gcccagcgtc ttcatcgtgg gcgacccgaa gcagagcatc
     1261 taccggttcc gccgcgccga gcccaaggtc ttcgaggctg cgcgtgattt cgtgaagcaa
     1321 ggccttgaag gacaggtgtt gagctgcgac cacacccgtc gcaatgctcc cgaggtgctg
     1381 gccgtgctca acagcgtgtt cgagcaggcg ggccgggacg gtgagttcgc cggtttccgt
     1441 ccgcacacca cggcgctgga gccggtgtcc ggtgcgggcg tctgcagctt gccgctcgtg
     1501 cttcgcgaag ccagggagcg cgacgacgca gccgatgggc aagtctggcg cgacacactg
     1561 tcggttccgc gtgtcgaacc tgaacagcgc ttgcgccagc aggaagcgga tcgcgttgca
     1621 ctggccgtgc gcgacgtcct tgcgggaggc gtgaagcccg gccaggtgca ggtgctgtgc
     1681 cgaaagcgtg aatcacttcg gctcgcggcc ggcgcactcg agtcgctcgg tgtgccctgc
     1741 gcggcggtcg aggaagccgc actgttcgat gcgcccgagg ttcgtgatct cgttgccgtg
     1801 ctcgatgtgc tggcatcccc cagccatgca ctgtcgctgg cgcaggcgct gaagagcccg
     1861 atcttcggtt gcagcgacga cgatctggtg cagctggcaa ctgctgtggc cgccagccgc
     1921 ggtggcggcg aggtgccccg tcgcccgccc gatccgctgg cctggtggag tgcactgcac
     1981 ggcgtagctg aacccagcgc ggcactgagg cgtgcccgtg cgttgttcgg cctgtggcag
     2041 caagctgctg ccgtgctgcc tccgcatgac ctgctggatc gcatcgtcga tgaaggcgag
     2101 gtgatgcagc gcactctggc cgcggtgcca ccgacgcggc gcgccgccgc gcgggctgcc
     2161 atccatgccc tgcttgcgca gtcactgatg ctcgatggag cgcgtggcgc gactccgtac
     2221 aacttcgtgc gggcgttgcg acgcaggcgc ctgaagatcg ctgcgcccgc gcagcccgat
     2281 gcggtgcagc tgctgaccat ccatggcgcc aagggtctgg aggccgaagt ggtcttcatc
     2341 atggacaccc aacccgaacc caatgtcgcc gacaccgcga ccctgctcgt gcagtggccg
     2401 gtcgaatcga gacatccggt gtgctgcgcc ttcgtctacg ccgagtcccg gtgcccgccg
     2461 ctgttgcaga ccttgttcga ggcagaggtg gcagcgcggc gacgcgagga actcaacagc
     2521 ctgtacgtgt cgatgacgcg tgccaagcac cgcgtggtca tcagtgccac cgtgccacat
     2581 cacgcgtcga aggtgtcctg gtggcagcgt ctggagccgt acgcgcccgc ctggccctcc
     2641 gaaccagctg tctgcgcgca cgccgacgag ccgggggccg acctgagttt gccggtgctg
     2701 cctgcctggg atccgccgcg cctggccgcc gccgtgccgg ctgagcgggt tcgggacaag
     2761 ggcgacgaat gcagtgccgc cagccggctt ggccaggcag tgcaccgggt gctggaatgg
     2821 gcggtgcgcg gcgaactgcc gggttctggc gcgctcgatg ccctgggtgc cgctgcggcg
     2881 cgagagttcg gcgccgagcc tccggcagtg cagcgtgtcg cggtcgcgat cctgcgcagt
     2941 gcggcgtgcc agcgcttctt cgatgctgcc ggcttgatct gggcgggcaa cgaggtcagc
     3001 gtgaccgacg gtggcgaggt cctgcgcatc gaccggctgg ttcacctgcg tgaggcgggc
     3061 ccgggtgtcg cctcgcaacg ggcgtctggc ggggtctggt gggtcctcga ctacaagctg
     3121 agcgaggcgc cgcaggaact tgacttgcac cggctgcaat tgcagcgcta ccagcgggcg
     3181 gtttcgcgcc tgcagccgca agccatcgtg aaggcggcct tcatcaccgg ccggggtgcg
     3241 gtcgtcgagc tggactgagg tggctcaccc gccgcctggg tccagcctgc ggccgggccg
     3301 aagctgctga aaacaaggcc tggcgagtca aatgcacggc aaacgcccgt gaattcatcg
     3361 cttggcgcct gccggggtcc atttccaata gaaagcatcc gatatccctg accatgaccg
     3421 gcaatccccc ttcctccgcc agcgcacgac cgagccagct gggtcgcaac ttcggccgct
     3481 tcgaactgct ccaactgctg ggcaagagcc agggcagcat ggtgtggctg gtcaaggatc
     3541 cgcatctcca gcaggagctg gtgctcacgc tgccgagagt gcagccggtc gacccggcgg
     3601 cactcgagcg ctggctcatg ccagtgcgcc atgcggcacg cctcgttcac ccgaacttgg
     3661 cgcagggcgt cgaggttgcc gtccaggaac actggccgta cgcagccgtc gaccgatcga
     3721 agggtgtcac gctgggcgaa tggctggctc tccatcccaa gccttctccg atcgaagcgg
     3781 tgggctgggt ctgtcaggtg ctggaagcgc ttgcctttgc ccacgaggcc ggtaccgccc
     3841 atcacgatct tcaactgcat agcctgctga tcaacgacgc aggccacgtg caattgatgg
     3901 ctctgtccac ttccagcacc cagggaacgc cagcagccgc cgatggtgtc cggcgcggtg
     3961 tcgatcaggc ggtgctggtc gaagccgaca ccctgcgtgc gcaacgcgaa gccgcggaac
     4021 gcgatgtgct ggcctgcggc ctgctgctgc acctgctgct gaccggcgct ccggtgctgg
     4081 acgaacccga tatctccaag gccatgctgc gcttgctgcc ggtgggccgc gacctggtgc
     4141 gcctgccctg gagcaccccg catcccattc ccgaggccct gcgtgcgatc gccaaccgct
     4201 gcacctcgag ccaggatcgc cagcgctatc taagtgcgcg caccttcttg cgcgcactgc
     4261 aaggctggct cgaagccgaa gcgctcggtc gcgacggccc gctcgaactg atgctcgacc
     4321 ggctgcgcac cgtcggcgcg ttgcccgcct tgcccggcat gagtgcccgt gtcgagcgca
     4381 tggccgcgat ggagggacag catgtcgagg aaatcgcgag attgatcctc caggacatgg
     4441 cgctcagttt cgaactgctg cgcgaagtca actcggtgca tgtacagggt acgcaggccg
     4501 ccggatcggc gcccgtgctg acgatccgtc gctcgatcgc gctgatcgga ctgaatggcc
     4561 tgcgccaggc ggccaacggg ttgcgtctgt ggccgggccc gctttccgag accggtgcgc
     4621 aggcgctgcg ttcgctgatg gaccgggtgc gtctggccgg ctacaccgcg caggccatgc
     4681 gaccggccgg gtacgacgcc gaagtcgtct acctcgtgac ggtgctgcag aacctgggcc
     4741 gcctgctggc tcagtaccac tttccggacg aattcgaaca gatccgccac ctgatgcagg
     4801 gcacgccggc acccgccgat gcaccgccgg gaactttggg cgtaccgggt tgggctgaat
     4861 ccgatgcctc tttcgcggtg ctcggcatcg acatcgaggc gctcggcgcc gccgtcgcgc
     4921 gccactgggg cctgggagag gaaatgctcc acatgatccg tcgcttgccg aaggatctcc
     4981 ccgtgcgcat gcccgacaac gacagcgatg tgcttcgcat caccgccagc gctgccaacg
     5041 aagccgtcga tgcatccctg atgcctgccg cagcgccccg gtcctctgca gcgacggcag
     5101 gggtggctgc cgatggcagc gccatgccat ccagcgctgc caagggatcc cgcggtgccg
     5161 gaccccgcac ctccgtcgct cagcgctacg cgcgcagcct cggcctgacc ccgcgcatcg
     5221 tgcaggagtc actggctgcc gctcgccagg ccctacgcac cggagagccg gtccagacca
     5281 gtgcttcgcg cgtcgaagat gccccagctg ccgagcaggc cgccgtggct gcgaactgat
     5341 cccgaccatg gcgagccttg ccgcgccgcc ggctgtccag cgcctgggcc gcttcgagtt
     5401 gaggcgactg ctgggcaagg gggcccaggc caacgtctgg ctgggtttcg acacgcatct
     5461 cgagcgcgag gtcgcggtca agatcatccg caacggcagc ggcgtcgccg cgggagatgc
     5521 cagccaatgg ctgcaggaag ctcgaagcgt cagccgcctg acccatccga acatcgttcc
     5581 ggtgttcgag gcggatgtgc acgaggggca accgtttctc gtcttcgagt acgtggcggg
     5641 ccacacgctg accgaagtgc ttcgcgcgaa aggcgcgttt gcgccgcagg ccgcagtggg
     5701 catcgtcctg gacatcctga gcgcgctgcg ggcagcccac gagactggaa tcgtgcaccg
     5761 tgacctgaag ccctcgaacg tcctcatcga catcggcgga cgggctcgcg tgatggactt
     5821 cgggatcgcg gtcaagacga acgacaccac tcagaagaac cagatcgtcg gcacgcccgg
     5881 gtacctctcg ccagaggccg cggaaggcct gtcgccgact ccggcgatgg atgtcttctc
     5941 ggccggactg gtgctggccg aattgctggc cggcaaggcg gtgatttccg agaaggatcc
     6001 ctatcgagcg atctaccgcg ccgcccatga ggatctggcg ctgccggaca gtgtcggcgc
     6061 tgaagtcgat gatgcgttgc gcgcgatcgt gcttcgcgcg atgtcgcgcg atcccgcacg
     6121 ccgatatgtc ggtgccgaag ccttcggcga cgccttgcaa gcctggatga atcccgtggc
     6181 gaatgaatcc gaggccgctg cgaccggagc cgcagccacc aacggaacgc ttgatttcct
     6241 gctgcgacgg atgcgccaca agagcgattt cccggcgctc tcgagtgccg tgtcgtgcat
     6301 ccatcgcgtg gccaactccg aaaccgaaag cctgaacagc ctggccaacg agatcctgaa
     6361 ggacgtcgcg ctgaccaaca agctgctgcg actggtcaac accgcaagct atcgccatgc
     6421 cggcggcggc tccatcagca cggtctcgcg ggccgctgcg ctgatcggat ttgccggcat
     6481 ccggaacatg gcgctgagcc ttgtgctgct ggaacacatg caggacaagg gacatgcgaa
     6541 cctgctcaag gaagagttcc tgcgttctct gatggcagga accctggcgg gcgagctctg
     6601 caggacgggg cgtgacagcg aggaggcatt catcgggtcg atgttccaga acctcggccg
     6661 actgctgacc cagtactact ttccagagga ggcacgccag gtccgcagct tgctcagccg
     6721 cgcagcggca tccggcgatg cgccgaagcc gggggcagag aactcggttg ccatccaggt
     6781 gctgggcctg agcttcgagg acctgggcgt cggcgttgcc aaatcctggg gactgcccga
     6841 cagcttgcag cgttgcatgc gcaagccagg cggcgagccc ccgtcgcgtc cgcccgatgc
     6901 cgctgcggag cggctgcgct ggcttgcgat ggctgccaac gacttgaccg acacgctgat
     6961 gcgctccgat gccgcggcat cggcgggcgc ccttgcagac gccgcagagc gctacgcccg
     7021 tgtcctcgga gtcagcgcga agcacatctg cgaaaccact gccgtggcgc ggatcaagct
     7081 ggccgaatcg gcgcgggcaa tgaatctggt ggtcggcaag aactcgctgg ctgcccggct
     7141 catggcgcca gcgcccggca cggcttcctc gggcctgctg gacgaatcca cgcagcccaa
     7201 caccgattcg ctgtcgcccc acgagttgca tgccacttcg ctgctgtgta cctctgcagc
     7261 cgacgacgct gcggcggcac cgatgggaac cccgggcagg gccgccgaag tgcttgccgc
     7321 aggcatccag gacatcacca attcgatggt cgacagcttc aagctcaacg aggtgttgcg
     7381 catgattctc gaaaccatgt tcagagccct cggctttcgc cgcgtgctgt tctgcctgcg
     7441 tgaccccaag accgaaacgc tgaccgggcg cttcggtctc ggcgagggtg gtgatgccgc
     7501 ggcagccttg ttcaaggtgc ctctgaaagg cggaaccgac ctgttcgccg cggtctgcct
     7561 gaaaggcgcc gacacgttga tacgcgacgc caccgaggcg gccatggccg agcgcttgcc
     7621 aagttggtat cgaaagacgg tcaacgcccc gtcgttcctg ctgctgcccc tgatgatgaa
     7681 gggctcgccg ttcgcaatga tctacgctga ccaggcccag ccgggtggca ttgagctgga
     7741 cgagaaggaa ctgtcgctgc tgcgcaccct gcgcaaccag gcggtgatgg cgttc
//
LOCUS       DAOORV010000154         7763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11596_length_7763_cov_8.116243, whole genome shotgun sequence.
ACCESSION   DAOORV010000154 DAOORV010000000
VERSION     DAOORV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11596_length_7763_cov_8.116243"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKV56_15365"
     CDS             complement(<1..385)
                     /locus_tag="PKV56_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOX69238.1"
                     /translation="MDFLIPLVSADFLGKPAWVWLAFVGIVFTLLAFDLGVLHNDDHE
                     IGVRESLLLSAGYVSVALLFGAWVWWYLGPQSGMDYYTGYMIEKSLSMDNVFVIALIF
                     GFFAIPRQYQHRVLFWGILGVIVLRA"
     gene            complement(396..554)
                     /locus_tag="PKV56_15370"
     CDS             complement(396..554)
                     /locus_tag="PKV56_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69239.1"
                     /translation="MDTATLLLAIMTVTLSAVTFHAWRLGNEKRDVALIGAVGGLCGV
                     GTAFAAFL"
     gene            complement(672..839)
                     /locus_tag="PKV56_15375"
     CDS             complement(672..839)
                     /locus_tag="PKV56_15375"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HOX69240.1"
                     /translation="MGRAQQRRQTRPADGLDQSGANPSLYFGMSLELTSPLNILVGMP
                     ACVHVAQRVLS"
     gene            complement(799..1215)
                     /locus_tag="PKV56_15380"
     CDS             complement(799..1215)
                     /locus_tag="PKV56_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69241.1"
                     /translation="MFYAFSWSVVIGLLVLWSLAAWAFHSITAWVTSHAGVLAAGSGS
                     TEGLQLPDWLVPWVPPEVASALDSMASALAPAIETLLEWAPALQGGLSVAVWLVWGLG
                     SILLIVLGLLATGLIATLRRRSIGGTGPAATTNAPG"
     gene            1329..2210
                     /locus_tag="PKV56_15385"
     CDS             1329..2210
                     /locus_tag="PKV56_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOX69242.1"
                     /translation="MLNYRHLYYFWVVTKEGGFARAAERLDMAVQTISAQVRELEKSL
                     GHQLLKPAGRGVAMTDAGHAAFARAEEIFQLGQLIPEEVREAASSKVARLAVGLSDGI
                     SKLAAHALLESVLSTPSLRLVCHEGEFEQLLGELALHHLDLVLTGQPAPRNPNLRLTS
                     ERLAESPVDWYGPAAHVHKADVDQFPQSLNRLPVLLPTGHSALRPTLDRWFEAQGLRP
                     HIVGEFEDSALLAVFAARGLGVFPVSRLGAGDVGLMRGLRLLGRCEDVKEEIHAIRSR
                     RGQHHPLVLQVLAAARA"
     gene            complement(2221..2655)
                     /locus_tag="PKV56_15390"
     CDS             complement(2221..2655)
                     /locus_tag="PKV56_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOX69243.1"
                     /translation="MRSYPRNSPEAAARIVALVLIADGHVCRSEFDALNHLDGAGQLG
                     LEPDGMPLVIQSLCEDLLMGAYAGGSMMACVDDGVMASLMAEVDEPALQRKVLQLAVA
                     AASADRHLADGEAVVIAAVRRYWGLGEEAGTESRIAADTQAA"
     gene            2939..3280
                     /locus_tag="PKV56_15395"
     CDS             2939..3280
                     /locus_tag="PKV56_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOX69244.1"
                     /translation="MQVLFKSRHPQATDLRELTERRVRFVLRRLGWLVPRAEVQMSDV
                     NGPRGGIDKRCQVELRTDGAGWVVVSSVANDWRTALDNALARAARFLMRLWRRGSDSR
                     RMRQRFISHER"
     gene            complement(3539..4069)
                     /locus_tag="PKV56_15400"
     CDS             complement(3539..4069)
                     /locus_tag="PKV56_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69245.1"
                     /translation="MTTSTVSPALQRLATEGRLLKPTLNGPTHVPGRCGFRGDIALTF
                     DPPAALEGAFAVADGDEPTLSFLAGSLSTMRLLPALAEALGGSVKAGGKYFVYVSDLE
                     RASRYQIELADVSYYVLPIDDTSVYNELIDYLYLDKTRMKKMDTAEKVDAIADGAIKY
                     SQKYETISYEEVIRRL"
     gene            4180..4452
                     /locus_tag="PKV56_15405"
     CDS             4180..4452
                     /locus_tag="PKV56_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69246.1"
                     /translation="MHIPGPARNPPEKQAPSRRPLGLGRRDGGSWSTEDYPFEHSDAV
                     LDGRLIGRVIDQRTQGMLTEARLDGWVAQVGPLLQPRLRQWEQPTA"
     gene            4679..5107
                     /locus_tag="PKV56_15410"
     CDS             4679..5107
                     /locus_tag="PKV56_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOX69247.1"
                     /translation="MTLETLRKIYERTLATPDAQTPGNAAECMFIHRTAVIMADGEVV
                     TCANMYAENVGHLERAATFGEIWNNDRMQSVRGALGSADEWSQCTRCWFREIRYGEQR
                     AQWASNGSPAAQESLIRPAKYQPISWDFRDQQRGRKRDDR"
     gene            5097..6266
                     /locus_tag="PKV56_15415"
     CDS             5097..6266
                     /locus_tag="PKV56_15415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX69248.1"
                     /translation="MTAERIELQSLGLFRVRKTDRFIELTLSLSCESGRYDYSKLYFT
                     RGVFDRYSEDRVLCFKMRQAGAPETIRLAVPPDALKSGWMRLRLEAFPGTSGRCTVLA
                     ARLVPEDGNALSGLAYLFAIKEWVRRQVAQSESLGRAVAPHPPESLSLELTAGCNFQC
                     SHCSSHGDEQEHRMNNRRLSFTREMLDKLAHEVFPFLTLVNLVGRGEPTMVPTDLWNR
                     LFELCETYRVLLTCVTNGSFVKHRFDLEKLRQLDTLTFSIDGMTSEVFAANRGGADLG
                     AFMDNLAHFQSLRRDPSLLRPPRLGLSWTLKRNNIEQFPDFIRFAIAADADLLYVRHL
                     LLFRPDDAAQSLVDDPDLCNRYLSAGYALLKGTRIKLDVPPLSEVSVHSSQVAAR"
     gene            6277..7086
                     /gene="murI"
                     /locus_tag="PKV56_15420"
     CDS             6277..7086
                     /gene="murI"
                     /locus_tag="PKV56_15420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOX69249.1"
                     /translation="MQKQPAHSAPIGVYDSGVGGLAVLRALKERLPGESFIYVADSAN
                     APYGDRSSEFVESRAREVAGFLVERNAKALVLACNTASVVAAQRLRALHDLPIIAMEP
                     AIKPATLITQSRVVLVLATTNTIRSESVARLCNLYGTGTRIILQPCPGLADRVEHGQF
                     RHIETRQMLEQYMRPGLIAGADTIVLGCTHYAFLADEIAAVAGPAVTIVEPSDAIARQ
                     LSRVLPAAPMLPNGVATTSIYTTGSTQQMSAFLASVGEASQRVYLLPTGPR"
     gene            complement(7199..>7763)
                     /locus_tag="PKV56_15425"
     CDS             complement(7199..>7763)
                     /locus_tag="PKV56_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69250.1"
                     /translation="LDRDPGPVDEPAALRDTIHAFFEQRARTTRSRLRQLLGIGRISF
                     LVGLVFLTVAVGLGDFIASAMTGQRLGELLRQGLVICGWVAMWRPLEIFLYGWWPIRA
                     EALLHDRLARMPVRIVYGGTARPGAWRHDWPAVTPKQTLAQKSPPHAESSVLVADEPE
                     AAGGTAERGPIASTTTDTVGLHTGAIR"
BASE COUNT         1377 a         2517 c         2537 g         1332 t
ORIGIN      
        1 tggcgcgcag cacgatcacg cccaggatgc cccagaacag cacgcggtgc tggtattgcc
       61 gcgggatcgc aaagaagccg aagatcagcg cgatcacgaa gacgttgtcc atcgacagcg
      121 acttctcgat catgtagccg gtgtagtagt ccatgccact ctgcgggccc agataccacc
      181 agacccaggc accgaacagc agggcaacgc tgacgtagcc ggcggaaagc agcaggctct
      241 cccgcacgcc gatctcgtgg tcgtcgttgt gcaggacgcc cagatcgaac gccagtagcg
      301 tgaacacgat gcctacgaag gcaagccaga cccaggcagg cttacccagg aaatcagccg
      361 agacgagggg gatcaggaag tccatagggg tgacctcaga ggaatgcagc gaaggccgtg
      421 ccgacgccac acaggccgcc cacggctccg atcagggcca catcacgctt ctcgttgcca
      481 aggcgccagg cgtggaaggt cacggctgac agcgtgacgg tcatgatcgc gagcagaagg
      541 gtggcggtgt ccatgttttc tgttgatgtg caagaggtga gggatcgtcg gcggtttggt
      601 gaccggcagg aatcagcgtc ggcgtgaact caggttcgga tttcccgaag cgaagccgca
      661 ggaccgtccc ctcatgacag aacccgctga gccacgtgga cgcaggcagg catgccgacg
      721 aggatgttca agggggacgt caactccagc gacatgccga agtacagcga cggattggct
      781 ccggactgat cgagcccgtc agccgggcgc gtttgtcgtc gctgctgggc ccgtcccacc
      841 gatcgaccgt cggcgcagcg tggcaatcag cccggtggcc agcaacccca gcacgatgag
      901 caggatgctc ccgaggcccc agaccaacca gaccgcgacc gacagtccgc cctgcagtgc
      961 gggcgcccat tccagcaagg tttcgatggc gggtgcgagg gcggaggcca tcgaatccag
     1021 ggcagaagcg acttcgggcg gaacccatgg cacaagccag tccggcaatt gcagaccctc
     1081 ggtcgaaccg gagcctgcag ccagcacgcc cgcgtgggag gtgacccatg ccgtgatcga
     1141 gtgaaaggcc caggccgcca gagaccagag cacgagaagg ccgataacca cagaccagct
     1201 gaaagcgtag aacatgatcg attcctcgtt gaaagaccgc aacagggggt gagtctttcc
     1261 tactcatcca ttcgaataaa gcagcgtcgg gaatacttgg acttctgttt ttccgatgcg
     1321 agatgggcat gctcaattac cgccatctgt actatttttg ggtcgtcacc aaggagggcg
     1381 gattcgcccg tgcagccgag cggctggaca tggcggtgca gaccatcagc gcgcaggtgc
     1441 gcgaactcga gaagtcgctt ggtcaccagt tgctcaagcc tgccggccga ggcgtcgcga
     1501 tgaccgatgc gggccatgcc gccttcgccc gcgcagagga aatctttcag ctgggccagc
     1561 tgatccccga ggaagtgcgg gaggcggcca gcagcaaggt cgcccgcctg gccgttggcc
     1621 tgtcggacgg catttccaag ctggctgcac acgcgctgct cgagtccgtg ctgtccacgc
     1681 catcgctgcg cctggtctgc cacgagggcg agttcgagca actgctgggc gaactggcgc
     1741 tgcatcacct ggacctggtg ctgaccgggc agccggcgcc gcgcaacccc aacctgcgcc
     1801 tgacgagcga acggctcgcc gagtcgcccg tggattggta cgggccggcg gcccatgtgc
     1861 acaaggctga cgtggatcag ttcccgcaat cgctcaatcg attgcccgtg ctgctgccga
     1921 ccggccattc ggccctgcgc cccacgctgg atcggtggtt cgaggcgcag gggctgcggc
     1981 cgcacatcgt cggcgagttc gaggacagcg cgctgctcgc ggtatttgct gcgcgcgggc
     2041 tgggggtctt cccggtcagc cggcttggcg cgggtgatgt gggcttgatg cgcggactgc
     2101 gcttgctcgg gcgctgcgag gatgtgaagg aggagatcca cgccatccgc tcacgccgcg
     2161 gacagcatca ccccctggtg ctgcaggtcc tcgccgcggc gcgcgcctga cctctgtcgc
     2221 tcaggcggcc tgcgtgtcgg cagcaatgcg gctctccgtg cctgcttcct caccgaggcc
     2281 ccaatagcgg cgaaccgctg caatcaccac ggcctccccg tcggcgagat gccggtcggc
     2341 actcgcagca gcaaccgcca actgcagcac cttgcgctgg agcgcgggct catccacctc
     2401 ggccatcagc gatgccatga cgccatcgtc cacgcaggcc atcatcgacc cgccggcgta
     2461 ggcacccatc agcaggtcct cgcacagcga ttggatgacg agcggcatgc cgtctggctc
     2521 cagacccagc tgacctgccc catcgagatg gttcagtgca tcgaattccg agcggcagac
     2581 atggccgtcg gcaatgagga cgagcgccac gattcgtgca gcggcctcgg ggctgtttcg
     2641 ggggtaggag cgcatggcaa tgttctttcc tgaatggtgg agggtgtgtg tgggcgagac
     2701 gcggcgggca gcagtggggc ggttggacag agctttcatg gccgggcctc ggggaagatc
     2761 gaacagccgt gaatgtggat gcgatgctga ggatgaaata gcagtttttc cacgaagcca
     2821 ggttcggcaa aagcgaactg ttcaggtttg tccgatgtcc tcgttctgta aagacttctt
     2881 ttccagaacc atcaggagtt ccagagttcg tccatccctc atcccctgga gattcgtgat
     2941 gcaagtgctc ttcaagtccc gccatccgca agccaccgac ctgcgcgaac tgaccgagcg
     3001 ccgagtgcgt ttcgtgttga gacgcctggg ctggcttgtg ccccgagccg aggtgcagat
     3061 gtcggatgtg aacggcccgc gtggcggcat cgacaagcgc tgccaggtgg agctgcggac
     3121 cgatggcgcc gggtgggtgg tcgtctcttc cgtggcaaac gactggcgca ctgcgctgga
     3181 caacgcgctg gcgcgtgccg cgcggttcct gatgcgcttg tggcgtcgcg gaagtgactc
     3241 ccgccgcatg cgccagcgct tcatcagtca cgaacgctga acccgggcga cccgaggaac
     3301 aggcgccatg aaccccatcc ctcgacctct cgccacggct ctgcggaacc gctcccggcg
     3361 gtctcaggcc ttgcggggcc atccatcgtc gtcggcgcga gccgggtgct gcgcaacacg
     3421 tacgcattgc tgtcgatgac gtctcgctgc aggcacccgg tccgaatggc accttcccgc
     3481 cattcgtgga ctcgggtcgc gcgactggcg tcgcgatccc tacacggccg gtaaccggtc
     3541 agagccggcg gatgacttcc tcgtagctga tggtttcgta cttctggctg tacttgattg
     3601 caccatccgc gatggcgtcg accttctcgg cggtgtccat cttcttcatc cgggtcttgt
     3661 cgaggtacag gtaatcgatc agctcgttgt agaccgaggt gtcgtctatg ggcagcacgt
     3721 agtacgagac atccgcgagt tcgatctggt agcgcgaggc gcgttcgaga tcggacacgt
     3781 agacgaaata cttgccgcct gccttgacgc tgccaccgag cgcctctgcc agcgcgggaa
     3841 gcaggcgcat cgtggacagc gaccccgcga gaaacgacag tgtcggctcg tcgccgtcgg
     3901 ccacggcgaa ggccccttcc agcgccgctg gcggatcgaa ggtcagcgca atgtcaccgc
     3961 gaaagccgca gcggccgggc acatgcgtgg gcccgttgag cgtcggcttg agcaagcggc
     4021 cctcggttgc gagacgctga agagcgggtg atacggtgct tgtggtcatg cggggtcctg
     4081 atgcaatcgc ccccacgcgg cgatgcgtgt caggcgggtt gcgggttgag aaacggcgtg
     4141 cagaaacgga agctggggca gcacgcctga gcactcccta tgcatatacc gggccccgcc
     4201 aggaacccac ccgagaagca ggcgccgagt cgacgtcctc tcgggctggg gcgccgagat
     4261 ggtggcagtt ggtcgaccga ggactatccc tttgaacact ccgatgccgt gctggacggg
     4321 cggctcatcg gtcgggtgat cgatcagcgc acgcagggca tgctcaccga agcacgactg
     4381 gatggctggg tggcacaggt cgggccgctg ctgcagcctc gcttgcgtca atgggagcag
     4441 ccgacagcgt gagcgatgcc tgatcgcgag cacttcttca gcctatccct agccgaccca
     4501 ggtttgctgg aatcgacgcc tcctcttgcg cgcgctgcgc aggcgttcat cgggcggccg
     4561 gcccgggggt ggcgaccatt gccagcaccg tcatcatcac gtcgagtgcc gaggctatat
     4621 tgccaagcga ctgcccgtgg ccgagtccag ccgtgagtca gacaggtgca ctccgcgaat
     4681 gacgctagaa accctcagga agatctacga acgcacgctg gcgacgccgg atgcgcagac
     4741 cccgggcaat gccgcggagt gcatgttcat acaccgcacg gccgtgatca tggccgacgg
     4801 cgaagtggtg acctgcgcca acatgtatgc agagaatgtc gggcacctcg aacgggccgc
     4861 gacattcggg gagatctgga acaacgaccg catgcagtcc gtgcgaggcg cgctgggctc
     4921 cgcggatgaa tggtcccaat gcacgcgctg ctggttcagg gagatccgct acggagagca
     4981 gcgcgcgcaa tgggcgagca acggcagccc tgccgcgcag gagtcactga tccggcctgc
     5041 gaagtaccag cccatctcct gggatttccg ggatcagcag cgcggcagga agcgcgatga
     5101 ccgctgaacg gatcgagttg cagtcgttgg gtttgttccg ggtcaggaag accgaccggt
     5161 tcatcgagtt gactttgagt ctgtcgtgcg aaagcgggcg ctacgactac agcaagctgt
     5221 acttcacgcg cggtgtgttc gaccgctaca gcgaggaccg ggtgctgtgc ttcaagatgc
     5281 ggcaggccgg cgcgccggaa accattcgcc tcgcggttcc gccagatgca ctgaagtctg
     5341 gctggatgcg cctgcggctc gaagcgtttc ccggcacgtc gggccgatgc accgtgctgg
     5401 ccgcacgcct cgtgcctgaa gacggcaacg cgctctccgg gctcgcgtac ctcttcgcta
     5461 tcaaggaatg ggtgcgccgt caggtggctc agagcgaatc gctgggccgg gccgtggcgc
     5521 cgcacccacc cgagtcattg agcctcgagt tgacggccgg ttgcaatttc cagtgttcgc
     5581 attgcagcag ccacggcgac gagcaggaac atcggatgaa caaccgtcgg ctgtccttca
     5641 cacgggagat gctcgacaag ctggcgcacg aagtcttccc attcctcacg ctcgtcaacc
     5701 tggtcggccg tggcgagccc acgatggtgc ccaccgatct ctggaaccgg ctgttcgagc
     5761 tctgcgaaac ctacagggtc ctgctgacat gcgtgaccaa cgggtccttc gtgaagcatc
     5821 gtttcgacct cgagaagctg cgccagctgg acacgctcac cttttccatc gatggcatga
     5881 cgagcgaggt gttcgccgcc aaccgcgggg gggccgatct gggcgccttc atggacaacc
     5941 tggcccactt ccagtcgctg cgcagggacc cctcgctgtt gcggcccccg cgcctgggcc
     6001 tgtcgtggac cctcaaacgc aacaacatcg agcagttccc tgatttcatc cgtttcgcga
     6061 tagcggccga cgccgacttg ctctatgtcc gtcacctgct gctgttccgg ccggacgatg
     6121 cggcgcagtc actggtggac gaccccgact tgtgcaaccg ctacctgtcg gcgggatacg
     6181 cgttgctgaa aggcacccgg atcaagctcg acgttccgcc actttcagag gtctcggttc
     6241 attcgagtca ggtggcggcc cggtagagtg cgggccatgc agaagcaacc cgcgcattcg
     6301 gcaccgatag gcgtctacga ctccggtgtc gggggcctgg ccgtgcttcg ggcgttgaag
     6361 gaacggctgc ccggggaatc cttcatctat gtcgccgaca gcgcgaatgc accctatggc
     6421 gaccggtcgt cggaattcgt ggagtcgcgc gccagagagg tggccgggtt cctggtcgag
     6481 cgcaacgcga aggcgctcgt gctggcgtgc aacacggcga gcgtggtcgc tgcgcagcgc
     6541 cttcgcgcgc ttcacgatct gcccatcatc gcgatggagc ccgccatcaa gcccgccacg
     6601 ctgatcacgc agtcgagggt ggtgctcgta ctcgccacca ccaacacgat acgcagcgaa
     6661 tccgtcgccc ggctttgcaa cctgtatggc accggcacgc gcatcatcct gcagccttgc
     6721 cccgggctgg cggatcgggt cgaacacgga cagttccggc acatcgagac ccgccagatg
     6781 ctggagcagt acatgcgccc gggcctgatc gccggcgcag acaccatcgt cctgggctgc
     6841 acccactatg cgttcctggc cgacgaaatc gcggccgtgg cgggcccggc tgtcaccatc
     6901 gtcgaaccct ccgatgccat cgcgcggcaa ctctcgcgtg tgctcccggc cgcgccgatg
     6961 cttccgaacg gtgtcgccac cacgagcatc tacaccaccg gttccacgca gcagatgtct
     7021 gccttcctgg cgtccgtcgg tgaggccagc cagcgggtct accttctgcc cacgggccct
     7081 cgctgaccct tcagctgcat cgaggtccga cggccaggag gcccacttgg cgcaaccttg
     7141 tgggtggctt ccgtggattc agggcccgct gtcgggcgcg ggcctgccgc ccgacgcgct
     7201 agcggatcgc gcctgtgtgc aagccaacgg tgtctgtcgt cgtcgacgcg atcggacccc
     7261 gctcggcggt gccgcccgcc gcctcaggtt cgtccgcgac gagcacggaa gattcggcat
     7321 gcggcggcga tttctgggcc agcgtctgct tcggcgtgac cgcaggccag tcgtgccgcc
     7381 acgccccggg tctcgccgtt cccccgtaca cgatgcgcac gggcatcctg gccaatctgt
     7441 cgtgcagcag ggcctccgcg cgtatcggcc accagccgta caggaagatt tccagcgggc
     7501 gccacatggc gacccagcca cagatcacca gaccctgcct gagcagttct cccagccgct
     7561 ggcccgtcat cgcgctcgcg atgaagtcgc ccaggccgac ggcgacggtc aggaacacca
     7621 ggccgaccag aaagctgatg cgaccgatgc ccagcagctg acgcaggcgg cttcgcgtgg
     7681 tccttgcgcg ctgctcgaag aatgcatgga tggtgtcgcg cagcgcggcc ggttcatcga
     7741 ccggccccgg atccctgtcc agg
//
LOCUS       DAOORV010000155         7609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11890_length_7609_cov_6.037066, whole genome shotgun sequence.
ACCESSION   DAOORV010000155 DAOORV010000000
VERSION     DAOORV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_7609_cov_6.037066"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKV56_15430"
     CDS             complement(<1..205)
                     /locus_tag="PKV56_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HOX69251.1"
                     /translation="MSSLPTAAAATPAARLRTSALPTPAKNVRSLSGLTPFLYPYRTR
                     IAWAVVFLVMAAVSTLVFPVALKS"
     gene            449..1333
                     /locus_tag="PKV56_15435"
     CDS             449..1333
                     /locus_tag="PKV56_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69252.1"
                     /translation="MKKTLAIGTALLIAATTSISPSLAVEQDVSIGEMLRRELPEKTK
                     SAVFVVYLFSHDLNSSIGRIPPASALNSDYFQRLSLEINRLIKASEDKDENQILGDAG
                     FRKELQYLVGKVRAVADPNITGVCQGLGIDLKTAKESRGDWRLVKAGNEPSEGKQAVG
                     VPIKPSSFLHIKLYWYYRLGCGGKKDLEMARRVLFEISDLEPVSLRERSDLRAEFRHC
                     EAETWAKYGIGGPVDLVRAEQFSKRYTKATFLLENEDTPEIRAEMTAQFPDAAKRNFG
                     CPRADYYRDPRDPWLRLW"
     gene            complement(1400..2491)
                     /locus_tag="PKV56_15440"
     CDS             complement(1400..2491)
                     /locus_tag="PKV56_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HOX69253.1"
                     /translation="MGLGSAATALASSAPTGVRPRPLEFPADHGSHPDTRIEWWYVTG
                     AVQPVVGAAGGENRGAQVDTCGFQVTFFRSRTDVAADHPSRFAARQLIFAHAALTDLG
                     RGRLQHDQRIARIGFGIAGAAVGDTDVSLRDWRLGRQARNGGSRYRALVSSDSAGFGF
                     DLQFDTVQTLLLQGDAGYSRKGPQPAQASHYYSQPQLAVSGSVTRDGKTLPVRGTAWL
                     DHEWSDSLLDPTAVGWDWIGMNLDNGGALTAFRLRGADGRPVYAGGSFRAAGGAVRNF
                     AASEVLLTPGRHWTSPGSQARYPVEWSLDCPAGRFRVRSLLNAQELDSRASTGSIYWE
                     GLSELLDEAGRRVGLGYLEMTGYAARLSL"
     gene            2668..6498
                     /locus_tag="PKV56_15445"
     CDS             2668..6498
                     /locus_tag="PKV56_15445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX69254.1"
                     /translation="MPEFSLPADFDLSDHFDHPTLMRAIGMKPEQALLSEHEDTGRLH
                     TRLRGSGTEVYEQTISFFVDPKTGLHLRGLCTCPVGFNCKHVAAALMAHEAALARARR
                     LGAGQTDGGGAESPAVASFASAGLPAVARSGRSGSSGDVRTTSASTVLPHALETWLGS
                     LGSAGAVIDARMTGERIAASESTGAASNKRLMYVLCSAEARLSLKLHLGSARRSGEIS
                     RHHPHAPSVADMLRTKPSYVGAADEALLAALLPFTLNRVGGLVPLRGRTAAAALQQLV
                     RAGACWFVGPEDALPDTPPGPPLTWVDEPLDLRLRWRTDEQGHWLTRWEAEPLAGDAT
                     AAGEPAPVVDALVLLPEPHAFVRDARGRPSVRPADVSSSGLTPPTIRWLAGMPAVPNH
                     ALPSLVNRLRVLGLAHQVVLPLPDEHAQPLQELGELPLQPVLRLLTCPNVPSETWLEG
                     HQRPPPAGQPLRQAAVQLLLRYELSAPGETRRGGSSVDFNFPAGASDVAFIEQPDPVR
                     PGQTRLVRFRRNAQAEAQALQRLFDRLELRPWSRVAAFALDRMAALKSARLQASALPP
                     ALDDGLLMLVPTTRDRWPTLLSQDVPALQADGWRIERADDFPFEVYEADDFEMSLDEA
                     TEGGTDWFRIGLKVSVGGSPVDLVPLLVGLVQAGWLKLEASLREPDGEVLLPWPDETA
                     PVVGAAQHAPGLRRERLLRLPVARIAPLVAWLRGVFSTSGFSAGEPKVSRFELGAIET
                     LSAGLALRAPPALDQLLDQLRLLRTGEGLPAVAVSPNVQAQLRPYQLDGLAWMDFLRR
                     AGFGGVLADDMGLGKTLQTLALLQAELDAGRLDRPSLVVVPTSLLENWQAEATRFTPQ
                     LRMLVLHGKQRAQQFKGIPGAHVVVTSYPLAVRDMAHLSSHAWHYLVLDEAQRIKNSR
                     SLATLALKGLNARHRLCLSGTPLENHLGELWSLMDFVSPGLLGNEAQFREHYRNPIEK
                     RQESLQAENLARRVRPFILRRTKLQVAQDLPEKTETLLRVELDGTQRDLYETVRATMD
                     SKLREAIARQGLARSQIVVLDALLKLRQVCCDPRLLKLRAASGELDESASEGDAAGSG
                     AVLAERPVHRHATSTAPSAKLELLRDLLPTLVEDGRRILVFSQFTEMLALIEHELDRL
                     DLAYVKLTGDTVNRGEVVRSFQEGNTPIFLISLKAGGVGLNLTAADTVILYDPWWNPA
                     VEQQAIDRAYRIGQGKPVFVYKLVASGTVEEKMLELQARKAGLADSLLSGVAGTAALT
                     AQDFDELFRPLGAA"
     gene            complement(6542..6772)
                     /locus_tag="PKV56_15450"
     CDS             complement(6542..6772)
                     /locus_tag="PKV56_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOX69255.1"
                     /translation="MHKPPESSRRTLSALALLLALSVTACGHLSHREQNTAVGAGIGG
                     VAGAVLTGGSTIGTVGGAMVGGVIGNRVTTKK"
     gene            complement(6930..7109)
                     /locus_tag="PKV56_15455"
     CDS             complement(6930..7109)
                     /locus_tag="PKV56_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOX69256.1"
                     /translation="MTLVATGCGSMSRRDENTAVGAGVGGVAGAVLTGGSTIGTVGGA
                     VIGGVIGNQVDPKKK"
     gene            complement(7324..>7609)
                     /locus_tag="PKV56_15460"
     CDS             complement(7324..>7609)
                     /locus_tag="PKV56_15460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOX69257.1"
                     /translation="IPGSVTPEKTDAVADALGRDASRMARRHEFDADAFALRTLRELG
                     YADAHALSALSRFGFQRDSATHPGTRKRLAALCAIDEPAEIETAAIEASR"
BASE COUNT         1263 a         2454 c         2574 g         1318 t
ORIGIN      
        1 gcgacttcag cgccaccggg aacacgagtg tgctgaccgc cgccatcacc aggaacacca
       61 ccgcccacgc gatgcgggtt cggtaggggt acaggaaggg cgtgagaccg ctcaacgagc
      121 ggacgttctt ggccggtgtg ggcagtgcgg aggtgcgtag cctggccgcg ggtgtcgccg
      181 cggcagccgt ggggagggaa gacatccgac gagtctacag agagccccca aggccgacgc
      241 tctgcgtttc attgagaggg ccgatcaaac ctgatttagc aacatcttcg gctcgtcttc
      301 gttcgccaaa tggatttgga tttggctaca gaatcgtgga tgcatggcag cgggaggtca
      361 tcggttatca gcgggcggtt cacgaagtca ggcgcctgtc tcctgcacat cactccagcg
      421 gcaaattcct gtcagcagca tgctcttctt gaaaaaaact ctagcgatcg gcacagcgtt
      481 gctgattgca gcgacaacct ctatctcacc tagcttggcc gtcgaacaag acgtaagcat
      541 cggggagatg ctgcgtcggg agctaccgga gaaaacgaaa tcagcggtat ttgttgtgta
      601 cctgttttct cacgatctca atagtagtat tggcaggata cccccggcaa gcgctctgaa
      661 ttctgattat ttccagagac tatctctgga aattaatcga ttgatcaaag cttcggaaga
      721 taaagatgaa aaccagattc ttggggacgc aggattcagg aaagaacttc aatatttggt
      781 tggaaaagtt cgagcagtcg ctgaccccaa tattacaggc gtgtgtcaag gtctggggat
      841 agacctcaaa actgcaaagg aatcccgagg agattggcga ttagtcaaag ccggaaacga
      901 accgagcgag ggaaaacaag cagtcggtgt gccaatcaaa ccgagcagct ttctccacat
      961 caagctttat tggtactaca gactcggttg tggtggcaaa aaggatttgg aaatggcaag
     1021 gagggtcctg tttgaaatct cagatttgga gccggtttcc ttgagggagc gttctgatct
     1081 tagggccgaa tttaggcact gtgaagctga aacgtgggct aagtatggta tcggcgggcc
     1141 agttgatctc gttagagcag aacaattctc gaagcggtac acaaaggcta cttttttgct
     1201 cgagaatgaa gacacgcctg aaatccgagc tgaaatgaca gcccaatttc ctgatgcagc
     1261 caagagaaat ttcggatgcc cacgtgctga ctactatcgt gaccctagag acccatggtt
     1321 gaggctgtgg tgatgttccc aggtatcaag tgatgttctg taagtcggcc gccgaaccgt
     1381 agttctcgtt ttccaatttc tacaacgaca gccgcgcggc atagccagtc atttccaggt
     1441 agccgaggcc gacgcgacga cctgcctcgt ccagcaactc gctcaggcct tcccagtaga
     1501 tcgagccggt gctggcgcgg ctgtcgagtt cctgggcgtt gagcagcgac ctcacgcgaa
     1561 atcggcctgc cgggcagtcg aggctccatt cgacggggta gcgggcctgc gagcccgggc
     1621 tggtccagtg gcgaccgggc gtgagcaaca cctcgctggc ggcgaagttc cgcacggcgc
     1681 cgcccgcagc ccggaagctg ccgccggcgt agaccgggcg tccatcagcg cctcgcagcc
     1741 ggaacgcggt cagcgcgccc ccgttgtcga ggttcatgcc gatccagtcc catccgacgg
     1801 cggtggggtc gagcagcgag tcgctccatt cgtggtcgag ccaggcggtg ccgcgcaccg
     1861 gcagcgtctt tccgtcgcgc gtcaccgagc cgctgaccgc gagttgcggc tggctgtagt
     1921 aatggctggc ctgcgccggt tgcgggccct tgcgcgagta gcccgcgtcg ccttgcagca
     1981 gcagggtctg cacggtgtcg aactgcagat cgaagccgaa tccggcgctg tcgctggaga
     2041 ccagggcgcg atagcggctg ccgccgttgc gcgcctgccg gcccaggcgc cagtcgcgca
     2101 gtgacacgtc ggtgtcaccg accgcggccc ccgcgatgcc gaatccgatg cgcgcgatgc
     2161 gctggtcgtg ctgcagccgg ccgcgtccca ggtcggtcag cgccgcatgc gcgaagatga
     2221 gctgtctggc ggcaaaccgg ctcgggtgat cggcggccac gtcggttcgc gagcggaaga
     2281 aggtcacctg gaagccgcag gtgtcgacct gcgcgccgcg gttctcgccg cccgctgccc
     2341 cgacgaccgg ctgcacggcg ccggtcacgt accaccactc gatgcgtgtg tcggggtgcg
     2401 atccgtggtc cgccgggaac tcgagcggcc gaggtcgaac acccgtcggt gcggagctgg
     2461 cgagcgccgt cgctgcagaa cctaacccca tcgccggcag cgctgcgatg gcttgcagcg
     2521 ctcgccgtcg acccgttcgg tgcagggcga cgagcgcatc gagaaacggc gtgtcacgag
     2581 aaatcacccc gacaaggatc gcacaggccg cattcgcccc ctggcctgac cgacaatgcc
     2641 cggttgccct gccgattgcg ctgtccgatg cccgagttct cactgcctgc cgacttcgat
     2701 ctcagcgatc atttcgatca cccgacgctg atgcgtgcca tcgggatgaa gcccgagcag
     2761 gcgctgctgt cagagcacga ggacaccggt cgtctgcaca cccggttgcg cggcagcggc
     2821 accgaggtct acgaacagac catcagcttc ttcgtcgacc cgaagaccgg cctgcatctg
     2881 cgcggcctgt gcacctgtcc ggtgggtttc aactgcaagc acgtggctgc cgcgctgatg
     2941 gctcacgagg cggcgctcgc gcgcgctcgg cggctcggcg caggtcagac cgacggcggc
     3001 ggtgccgagt cgcctgcggt ggcgtcgttt gcgtcggccg ggctgcccgc ggtggcccgc
     3061 tccggtcgat ccggctcctc cggcgatgtg cgcacgacgt ccgcatcgac ggtgttgcca
     3121 catgcgcttg aaacctggct gggcagtctc ggcagcgcgg gagccgtcat cgacgcccgg
     3181 atgaccggcg aacgcatcgc tgcctccgag tcgaccggtg ccgcctcgaa caagcggctg
     3241 atgtacgtgc tgtgcagtgc cgaagcccgg ctgtcgctca agctgcacct gggcagcgcg
     3301 cgtcgaagcg gcgagatcag ccgacaccat ccccacgcgc ccagcgtcgc cgacatgctg
     3361 cgcaccaagc cgtcgtatgt cggggccgcc gacgaggcac tgctggccgc gctgctgccc
     3421 ttcacgctga accgcgttgg tggcctggtg ccgctgcgcg ggcgtaccgc cgccgcggcg
     3481 ctgcagcagc tcgttcgcgc aggagcctgc tggttcgtcg gccctgaaga cgcgctgccc
     3541 gatacgccgc cgggcccgcc gctcacctgg gtggacgagc cgctggacct gcgtctgcgc
     3601 tggcgcaccg acgagcaggg gcactggctc acccgctggg aagccgagcc gctcgccggc
     3661 gatgcaacag cagctggtga gccagcgccg gttgtcgatg cgctggtgct gctgcccgaa
     3721 ccgcacgcgt tcgtgcgcga tgcgcgtggt cgtccgagcg tgcggccggc tgacgtgtct
     3781 tccagtggcc tgacgccacc gacgatccgc tggctggccg gcatgccggc ggtgccgaat
     3841 cacgcgctgc cgtctctcgt caaccggttg cgagtgctgg gtctggcaca tcaggtcgtg
     3901 ttgcccctgc ccgacgagca tgcgcagccg ctgcaggaac tcggtgagct gccgctgcag
     3961 ccggtgttgc ggctgctgac ctgtcccaac gtgccttcgg aaacctggct ggagggacat
     4021 cagcgtccgc cacctgccgg ccagccactg cgccaggcgg ccgtccagtt gctgctgcgc
     4081 tacgaactgt cagcgccagg cgaaaccagg cgcggcggct cctcggtcga tttcaatttt
     4141 ccagccggtg cgtccgacgt ggccttcatc gagcagcccg atcccgtgcg gcctggtcag
     4201 acccggctgg tgcgattccg ccgcaacgcg caagccgagg cgcaggccct gcagcgcctg
     4261 ttcgaccgcc tcgagctgcg gccgtggagc cgcgtcgctg cctttgcgct cgaccgcatg
     4321 gccgcactga agtccgcacg gctgcaggct tcggcgctgc ccccggcgct cgatgacggg
     4381 ctgctgatgc tggtgccgac cacgcgcgac cgatggccca ccttgctcag ccaggatgta
     4441 cccgccctgc aggccgacgg atggcgcatc gagcgcgccg acgattttcc tttcgaggtc
     4501 tacgaggccg acgatttcga gatgagcctc gacgaggcga ccgaaggcgg cacggactgg
     4561 ttccgcatcg gcctgaaggt cagtgtcggc ggctcgcctg ttgatctggt gccgctgctg
     4621 gtcgggctgg tccaggccgg ctggctgaag ctggaggcct cgctgcgcga gcccgatggc
     4681 gaggtgctgc tgccttggcc ggacgagacc gcaccggtgg tcggggctgc gcagcatgcg
     4741 ccggggttgc gccgcgagcg gctgctgcgc ctgccggtgg cgcgcatcgc accgctggtg
     4801 gcgtggctgc gcggtgtctt cagcaccagc gggttcagtg ccggcgagcc gaaggtctcg
     4861 cgcttcgagc tgggtgcgat cgagacgctg tccgcggggc tggccttgcg tgcgccgccc
     4921 gcgctcgatc agttgctcga ccagttgcgc ctgctgcgca caggtgaggg cctgccggcg
     4981 gtggccgtgt cgccgaacgt gcaggcgcag ctgcgtccct atcagctcga tggactggcc
     5041 tggatggact tcctgcgccg agccggcttc ggtggtgtgc tcgccgacga catgggcctg
     5101 ggcaagaccc tgcagacgct ggcgctgttg caggccgaac tcgatgcagg ccggctcgac
     5161 cggcccagcc tggtggtggt gccgaccagc ctgctcgaga actggcaggc cgaggcgacg
     5221 cgcttcacgc cgcagctgcg catgctggtg ctgcacggca agcagcgcgc gcagcagttc
     5281 aagggcattc ctggcgcgca tgtggtcgtc accagctacc cgctggcggt gcgcgacatg
     5341 gcgcatctct cgagccacgc ctggcactac ctggttctcg acgaggcgca gcggatcaag
     5401 aacagccgca gtctcgcgac cctggcactg aagggcctga atgcgcgtca tcggctgtgc
     5461 ctgtcgggta cgccgctcga gaaccacctg ggcgaactgt ggagcctgat ggatttcgtc
     5521 agcccgggcc tgctcggcaa cgaggcccag ttccgcgagc actaccgcaa cccgatcgag
     5581 aagcgccagg aatcactgca ggccgagaac ctggctcgtc gcgtgcggcc gttcatcctg
     5641 cgccgcacga agctgcaggt ggcgcaggac ctgcccgaga agaccgagac attgctgcgc
     5701 gtcgaactcg acggcacgca acgcgacctg tacgagaccg tgcgggcgac gatggacagc
     5761 aagctgcgtg aggccatcgc caggcagggc ctcgcgcgca gccagatcgt ggtgctcgat
     5821 gcgctgctga agctgcgcca ggtgtgctgc gatccgcggc tgttgaagct gcgggctgcc
     5881 tcgggcgaac tcgacgagtc ggcgtccgag ggcgacgcag cgggctcggg tgcggtgctg
     5941 gcggaaaggc cggtgcaccg ccacgcgact tcgaccgcgc cctcggccaa gctcgaactg
     6001 ctgcgcgacc tgctgccgac gctggtcgag gacgggcgcc gcatcctggt cttttcgcag
     6061 ttcaccgaga tgctcgcgct gatcgagcac gaactcgatc ggctggacct ggcctacgtc
     6121 aagctcactg gagacacggt gaatcgcggc gaggtggtgc gttcgttcca ggagggcaac
     6181 acgccgatct tcctgatcag cctgaaagcc ggcggcgtcg ggctgaacct gacggcggcc
     6241 gacacggtga tcctctacga cccgtggtgg aacccggccg tggagcagca ggcgatcgac
     6301 cgggcctacc gaatcgggca gggcaagccc gtgttcgtct acaaactcgt ggccagcggc
     6361 actgtcgagg aaaagatgct cgaactgcag gcccgcaagg cagggctcgc ggattcgctg
     6421 ttgtccggcg tggccggtac cgccgcgctg acggcccagg acttcgacga actgttcagg
     6481 ccgctcgggg cggcttgaag aagcgagggc aaggcagggt acctcgctct cgttgtggag
     6541 ctcacttctt cgtggtcacc cgattgccga tgactccacc gaccatcgcg ccgcccactg
     6601 tcccgatggt gctgcctccg gtcagcaccg cgccggcgac accgccgatg cctgcgccca
     6661 cggcagtgtt ctgttcccgg tggctcaggt ggccacacgc ggtgaccgaa agcgccagca
     6721 gcagcgcaag ggcggacaac gtccggcgtg aagattcagg gggcttgtgc atgaggggtc
     6781 aggatttgga tggccggaac tgcaccgttg cagttcgaag ccatgatgcc cgccgcttca
     6841 gccttcccgg gctgtcgcct cggtaagact ggtaaccagt tgaagcgcgc ggacgcggct
     6901 gacaggcggc ctcaagccgc cgcagcatct cacttcttct tcgggtcgac ctggttgccg
     6961 atgacaccac cgatgacggc accgcctacc gtaccgatcg tgcttccgcc ggtcagcact
     7021 gcgccggcca cgccgccgac gcctgcgccg acggcggtgt tctcatcgcg gcggctcatg
     7081 ctgccgcatc cggtggcgac cagggtcatc agcacggcga gggacaacgc ggggtaacgc
     7141 gagagggcga gggacttgtt catgttgatc tccggaatcg gggccaggag tcggcccgcg
     7201 gacttcgagg atgcgcgccg ctgtcgagtg gcgccatcgt cgcaagtcgt gttccggtgt
     7261 cagcggcggc ctacgtcgcg ccgagcgcgg ccggcgccat tcgcatttcc gagcggggaa
     7321 tgctcaccgg gatgcttcga tggctgctgt ttcgatctcc gcaggctcgt cgatggcgca
     7381 cagcgcggcg agccgcttgc gcgtgccggg atgggtcgcg ctgtcacgct gaaaaccgaa
     7441 acgcgacagt gccgacagtg catgcgcatc ggcgtaaccc agctcgcgca aggtgcgcag
     7501 cgcgaacgcg tctgcatcga attcgtggcg acgcgccatt cgggaggcgt cccgcccgag
     7561 ggcgtcggcc actgcgtccg tcttctccgg ggtcacgctc ccggggatg
//
LOCUS       DAOORV010000156         7605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_11901_length_7605_cov_6.204106, whole genome shotgun sequence.
ACCESSION   DAOORV010000156 DAOORV010000000
VERSION     DAOORV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11901_length_7605_cov_6.204106"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKV56_15465"
     CDS             complement(<1..802)
                     /locus_tag="PKV56_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOX69258.1"
                     /translation="MSIVEVKVPQLSESVAEATLLQWKKKPGEAVAIDEILVEIETDK
                     VVLEVPAPAAGVMAQIIKNDGESCVAEEVIARIDTAASPQVSPLAVKAVAPDAATPPP
                     PAPAGSAAGAAMPAAAKLMADNQLEPGSVAGTGKGGRVTKGDVTEALAAGAQAAAVAS
                     VAAKAPAPVAVAPAVARSLPSVSVPGPVNPGDRPEQRVPMSRLRARVAERLLQSQATN
                     AILTTFNEVNMAPVMEMRKRFQEKFEKEHGCKIGFMSFFVKAAVAALKK"
     gene            complement(932..3790)
                     /locus_tag="PKV56_15470"
     CDS             complement(932..3790)
                     /locus_tag="PKV56_15470"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOX69259.1"
                     /translation="MMQEYRANSHLFGGNAPYVEEMYEAYLDNPGSVPDNWRSYFDAL
                     QHLPAVDGSDERDVAHAPVVESFAQRAKANAFGAKASGADLAVARKQVHVQSLIAAYR
                     FLGSRWADLDPLKRQERPKIPELEPVFYDLTESDMDISFSASNTYFSTAENMTLRQIL
                     QALRETYCGSIGAEYMHVTDPTEKRWWQQRLESIRSKPTFSAEQKKHILEELTAAEGL
                     ERYLHTKYVGQKRFSLEGGESFIASMDELVQRAGSKGVQEIVIGMAHRGRLNVLVNTL
                     GKTPAELFSEFDHTAPENLPSGDVKYHQGFSSDVTTPGGPVHLSLAFNPSHLEIVNPV
                     VEGSVKARLDRRGDAAGRSVLPIIVHGDAAFAGQGVVMETLALAQTRGYFTGGTVHLV
                     INNQIGFTTSDPRDTRSTLYCSDVVKMVEAPVLHVNGDDPEAVVLATQMALDYRQEFS
                     KDVVVDIVCFRKLGHNEQDTPALTQPLMYKKIGQHPGTRRLYGDKLVTQGVLPADGPD
                     GMVKTFRAAMDEGKHSFSPVLTNYKSKYAVDWAPFLGKKWTDAADTALPLSEIKRLAE
                     RITALPKDFKVHPLVEKVLADRASMGRGEINVDWGMGEHLAFASLVASGYAVRLSGED
                     CGRGTFTHRHSVLHDQNREKWDEGTYTPLKHVAEGQAPYVVIDSLLSEEAVLGFEYGY
                     ASAEPNTLVIWEAQFGDFANGAQVVIDQFIASGEVKWGRANGLTLMLPHGYEGQGPEH
                     SSARLERFMQLAADNNMQIVQPTSASQIFHVLRRQMVRMFRKPLVILTPKSLLRAKDA
                     ASPLSEFTRGEFRTVIGPNLADVEADKVKRIIACSGKVAYDLMKRREAKKQNDTAIVL
                     VEQLYPFPHKAFASELKKFPNATEIVWCQDEPQNQGAWFFVQHYIHENMIEGQKLGYA
                     GRPASASPAVGYAHLHQEQLKSLLDQAFGKLKGFVLTK"
     gene            4041..4361
                     /locus_tag="PKV56_15475"
     CDS             4041..4361
                     /locus_tag="PKV56_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69260.1"
                     /translation="MKDHQTIPIHPEAPDKPPWGTKCNGCGVCCAAEPCPVGVLVTGS
                     RRGTCRALVWRQVDRRYVCGMVSEPGHYLGLNHPWLNAAASCVTRRLISAGSGCDSDA
                     ELIC"
     gene            complement(4372..5298)
                     /locus_tag="PKV56_15480"
     CDS             complement(4372..5298)
                     /locus_tag="PKV56_15480"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOX69261.1"
                     /translation="MPLPIIGAPLFIISHPQLVIEQCKSGVVGSMPALNARPISQLDD
                     WLAEITETLTAYNAANPHQRAAPFAINQIVHKSNDRLEHDMALCVKHKVPIIISSLGA
                     RTEINDAAHSYGGVVLHDVITNAFARKAIEKGADGLIAVASGAGGHAGVKSPFALVQE
                     IRDWFDGPLALSGAIATGGAVLAAQAMGADFAYVGSAFIATDEARACPAYKQMIVDGS
                     SDDIVNSNLFTGVHGNYLRGSIEQVGLDPDALPSGNSSEMNFGADAAKAWKDIWGSGQ
                     GIGAVKQVQPVCELVARLRMEYAQARARLALV"
     gene            complement(5337..7145)
                     /locus_tag="PKV56_15485"
     CDS             complement(5337..7145)
                     /locus_tag="PKV56_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOX69262.1"
                     /translation="MSYVAPVRDMLFCMKELAGLEGISNLPGFEDAGIDTAEAVLTEA
                     ARYCSEVVAPLNAEGDRHPPVWRSGEVSTSAGFRQAFRQYAQGGWQGLQHPIEFGGQG
                     LPKTIAAACSEMLNSASVSFALCPLLTDGAIEALLTAGNQEQRSFFLPRMIDGTWTGT
                     MNLTEPQAGSDLAMVRTRAVPQGDGAYRITGQKIYITYGEHDMAENIVHLVLARTPDA
                     PPGVKGISLFLCPKFLANADGSLGARNDVYCASIEHKLGIRASPTAVLVFGDDQGEVG
                     AGAIAQLIGEENRGLEYMFVMMNAARFSVGMQGIGVAERAYQRAVSYARDRVQSRPVD
                     ASLPAGAAIINHPDVKRMLMQMRAHIEGCRALASVSAAASDVAQRHPDAAVRQASQAF
                     HEFTVPLVKGFSTEMSIEVTSLGIQVHGGMGYIEETGAAQHFRDARILAIYEGTTAIQ
                     ANDLVGRKTLRDGGVTASSIAQQISDVELALSERASPAARAMHRNLQAARLAFVDVVD
                     FVLANTRTQPTRVYAGSVPYLMLAGKLLAGWQLARALLIAEDQLAEGGGDEPFLRAKI
                     ATARFFAEHVLSTVPGMRDAIIGGADSVNGLSVDSF"
     gene            complement(7209..>7605)
                     /locus_tag="PKV56_15490"
     CDS             complement(7209..>7605)
                     /locus_tag="PKV56_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOX69263.1"
                     /translation="GSEIAKNDRPELTAAKIVVSGGRALGSAEKFTEVMTPLADKLGA
                     AMGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVACGISGAIQHLAGMKDSKVIVAINK
                     DGEAPIFSVADYGLEADLFIAVPALVAAL"
BASE COUNT         1326 a         2454 c         2404 g         1421 t
ORIGIN      
        1 acttcttcag cgcagcaact gccgccttca cgaagaagct catgaagccg atcttgcagc
       61 cgtgctcttt ctcgaacttc tcctggaagc gcttgcgcat ctccatcacc ggagccatgt
      121 tgacctcgtt gaacgtggtc aggatggcgt tggttgcctg cgactgcagc agccgctcgg
      181 ccacacgtgc acgcagtcgc gacatcggca cgcgctgctc gggacggtcg cccggattca
      241 ccgggccggg taccgacacg ctaggtaggg aacgcgccac ggccggggcc acggcaactg
      301 gagccggcgc cttcgcggca acagacgcta ccgctgcagc ctgcgcaccc gcggccagtg
      361 cctcggtcac atcgcctttg gtcacgcggc cacccttgcc ggtgccggcc accgaacctg
      421 gctcgagctg gttgtcggcc atcaacttcg ctgccgcggg catcgctgct cctgcggccg
      481 aaccggccgg cgcggggggt ggcggggtgg ccgcatcggg tgccaccgcc ttgactgcca
      541 gagggctgac ctgcggcgat gccgcggtat cgatacgcgc aatgacctcc tcggccacac
      601 agctctcgcc gtcgttcttg atgatctggg ccatgacgcc ggctgctgga gcaggcacct
      661 ccagcacgac cttgtcggtt tcgatctcga ccaggatttc gtcgatggcg acggcttcgc
      721 ccggcttctt cttccactgc agcagcgtgg cttcagccac ggactcggac agttgcggta
      781 ccttgacttc aacgatggac atgatgttct ttctgggtgt tcaagcggct gccgtcccga
      841 tccgcgcggg cccgttcaac gaacggtggg aacggcatgc cgtggtttcg gcacacggcg
      901 tctgtcgtga cgccgctgct gacgttgcga ttcacttggt gaggacgaat cccttgagct
      961 tgccgaaggc ctgatcgagc agcgacttca gctgctcctg gtgcaggtgc gcataaccca
     1021 cggccggaga cgcggaagcc ggacgaccgg cgtaaccgag cttctggcct tcgatcatgt
     1081 tctcgtggat gtaatgctgg acaaagaacc aggcgccctg gttctgtggt tcgtcctggc
     1141 accacacgat ctcggtggca ttggggaact tcttcagctc ggaagcaaat gccttgtggg
     1201 ggaaaggata gagctgctcg accagcacga tcgccgtgtc gttctgcttc ttcgcttccc
     1261 tgcgcttcat caggtcataa gccaccttgc ccgaacaagc gatgatccgc ttgaccttgt
     1321 cggcctcgac atctgcgagg ttggggccga tgaccgtgcg gaattcaccc cgcgtgaact
     1381 cgctcaacgg cgatgccgca tccttggcgc gcagcagaga cttcggcgtc aggatcacca
     1441 gcggcttgcg gaacatgcgg accatctgac gacgcagcac gtggaagatc tggctggccg
     1501 atgtcggctg cacgatctgc atgttgttgt cggcggcaag ctgcatgaat cgttcgaggc
     1561 gtgcggacga gtgctcggga ccctgacctt cgtagccatg gggcagcatc agcgtgaggc
     1621 cgttcgcacg gccccacttg acctcgccgg aggcgatgaa ctggtcaatc accacctggg
     1681 cgccattggc gaaatcgccg aactgggcct cccagatgac cagcgtgttc ggctcggcag
     1741 aggcgtatcc atattcgaat ccgagcactg cttcctcgga gagcagggag tcgatcacga
     1801 cgtagggtgc ctgcccttcg gccacatgct tcaacggcgt gtaggtgcct tcgtcccact
     1861 tctctcgatt ctggtcgtgc aggaccgaat gacggtgggt gaaggtgcca cgcccgcagt
     1921 cctcacccga caggcgcacc gcatagccac tggcgaccag cgacgcgaag gccaggtgct
     1981 cgcccatgcc ccagtcgacg ttgatctcgc cacgtcccat ggaagcccga tcggccagaa
     2041 ccttctcgac cagcggatga accttgaagt ccttgggcag ggccgtgatg cgttctgcca
     2101 ggcgcttgat ctccgacagc ggcagcgccg tgtcggctgc gtccgtccat ttcttcccga
     2161 ggaagggggc ccagtcgacc gcgtacttgc tcttgtagtt ggtcagcacc ggactgaacg
     2221 agtgcttgcc ttcgtccatc gctgcacgga aggtcttgac cattccatcg ggaccgtctg
     2281 caggcaggac accctgggtg accagtttgt cgccatagag acgacgcgtg cctggatgct
     2341 ggccgatctt cttgtacatc aacggctgcg tcagcgccgg cgtgtcctgc tcgttgtggc
     2401 cgagcttgcg gaaacacacg atgtcgacca ccacgtcctt gctgaactcc tgccggtaat
     2461 cgagcgccat ctgcgttgcc aggaccacgg cttccggatc atcgccgttg acgtgcagca
     2521 caggcgcctc gaccatcttg accacgtcgg agcagtaaag cgtcgatcgg gtatcgcgcg
     2581 ggtcgctggt ggtgaagccg atctggttat tgatgaccag atggaccgtg ccaccggtga
     2641 aataaccgcg agtctgcgca agagccagcg tttccatcac gacgccctga ccagcgaatg
     2701 ccgcatcgcc gtgcacgatg atcggcagca ccgagcggcc ggcagcatcg ccgcgacgat
     2761 cgagacgggc cttgaccgaa ccttcgacga ccggattgac gatctcgagg tgagacgggt
     2821 tgaaagccag gctgaggtga accggaccgc cgggcgtggt cacatcgctg gaaaagcctt
     2881 ggtggtactt gacgtcgccg gagggcaggt tctcgggcgc ggtgtgatcg aactcggaga
     2941 acagttcggc cggcgtcttg cccaatgtgt tgacgagcac gttcaggcga ccgcggtggg
     3001 ccatgccgat gacgatttcc tgcacgccct tgctgccggc acgctgcacc agttcgtcca
     3061 tcgaggcgat gaagctttcg ccaccttcga gcgagaagcg tttctggccg acgtacttgg
     3121 tgtggaggta tcgctccagc ccttcggcgg cagtgagctc ctccaggatg tgcttcttct
     3181 gctccgccga gaacgtcggc ttcgagcgga tcgattccag gcgctgctgc caccagcgct
     3241 tctcggtcgg atcggtgacg tgcatgtact cggcaccgat cgagccgcag taggtttcgc
     3301 gcaaggcctg caggatctgc cgcaacgtca tgttctcggc ggtcgagaaa tacgtgttgc
     3361 tcgcgctgaa cgagatgtcc atgtccgact cggtcaagtc gtagaacacg ggctcgagct
     3421 cgggaatctt cgggcgctcc tggcgcttca acggatcgag gtcagcccag cgcgatccga
     3481 ggaatcggta agccgcgatc agggactgca catgtacctg cttgcgagcc accgccagat
     3541 cggcaccgct ggctttcgcg ccgaacgcgt tggccttggc gcgctgcgcg aacgattcga
     3601 ccacaggcgc atgcgccaca tcgcgttcgt cggagccgtc gactgccggc aggtgctgca
     3661 aggcgtcgaa gtacgaccgc cagttgtccg ggaccgagcc cgggttgtcg aggtaggcct
     3721 cgtacatctc ttcgacgtaa ggcgcgttac caccgaacag atgcgaattc gctctgtatt
     3781 cctgcatcat ctcgctcacc tcatgcgccg gtttccagcg cgatagtggg ttaaaaaacc
     3841 ttccgcgacc cggctgcacc ggtgggcgga caagcgaccc gactgagctg caaaacagct
     3901 gaggggacgg gaaggaccat tcaagtcacg gcggcgatgc taccaccggc gattgcagcc
     3961 cgatggccga cgaatgggag gtgttgcaga ccttattcgc agcgtcagcg tggtgaaaga
     4021 acagcgacaa ctgcatcggc atgaaagatc accagaccat acccatccac cccgaggccc
     4081 cggacaagcc gccctggggc acgaaatgca acggctgcgg ggtgtgctgc gcggccgagc
     4141 cctgcccggt gggtgtcctg gtgaccggca gccgtcgggg gacatgccgc gcgctggtgt
     4201 ggcgccaggt cgatcggcgc tacgtctgcg ggatggtcag cgagcccggg cactacctgg
     4261 ggctgaatca tccctggctg aatgcggcgg cttcttgcgt gacgcgaaga ctgatatccg
     4321 caggctccgg ctgcgattcg gacgcagaac tgatctgctg aacgttgccg ctcagaccag
     4381 ggccagcctc gcacgggcct gcgcgtattc catccgcagg cgggcgacca actcgcacac
     4441 cggctgcacc tgtttcacag caccgatccc ctgcccgctg ccccagatgt ccttccaggc
     4501 cttggccgca tcggcgccga agttcatctc actcgagtta cctgaaggca gcgcatccgg
     4561 atcgagaccg acctgctcga tcgaacccct caggtaattg ccgtgaacac cggtgaagag
     4621 gttgctgttg acgatgtcgt cactcgaccc gtcgacgatc atctgcttgt aggcagggca
     4681 ggctcgagct tcatcggtcg caatgaacgc cgatccgaca taggcgaagt ccgcacccat
     4741 cgcctgcgcg gcgagaaccg caccgccggt ggcaatcgct ccgctcaagg ccagcgggcc
     4801 atcgaaccag tcccggatct cctggaccag agcgaacgga ctcttcacgc cagcatgtcc
     4861 acccgctccg gatgccaccg cgatgaggcc atcggcgccc ttctcgatgg ccttgcgggc
     4921 aaatgcattg gtgatgacgt cgtgcaggac gaccccgccg taactgtgtg ccgcgtcgtt
     4981 gatctcggtg cgtgctccga gcgaactgat gatgatcggc accttgtgct tcacgcacag
     5041 cgccatgtcg tgctcgagcc ggtcgttgct cttgtggacg atctggttga tcgcgaacgg
     5101 tgccgcgcgc tgatgcggat tcgctgcgtt gtatgcagtc agcgtttcgg tgatctcggc
     5161 cagccagtca tcgagttgcg aaatcggtcg ggcgttcagt gccggcatcg agccgacgac
     5221 acccgacttg cactgctcga tgaccagttg cggatggctg atgatgaaga gtggcgcacc
     5281 gatgatcggc aagggcaggt tcttcagaat ctgcggaaac ggaaatcggg cagcgctcaa
     5341 aacgagtcga ccgacagtcc gttgacgctg tcggcaccgc cgatgatggc gtcccgcatt
     5401 ccgggaaccg tgctcaggac gtgctcggcg aagaagcggg ccgtcgcgat cttcgcccga
     5461 aggaaaggct cgtccccgcc gccttcggcc aattggtcct cggcgatcag gagtgctcga
     5521 gcgagttgcc agccagcgag caacttgcct gccagcatca ggtacggaac cgagccagcg
     5581 tagacacggg taggctgagt tcgggtgttc gccagaacga aatccaccac atcgacgaaa
     5641 gccaggcgcg cggcctgcag gttcctgtgc atcgccctcg ccgcagggct ggcgcgctcg
     5701 gacagcgcga gctcgacatc agaaatctgc tgcgcgatgg acgatgcggt cacaccaccg
     5761 tcgcgcagcg tcttgcgtcc gacgaggtcg ttggcctgaa ttgccgtggt gccttcgtag
     5821 atggccagga tgcgggcatc gcgaaagtgc tgcgctgcac cggtttcctc gatgtagccc
     5881 atgccgccgt gcacctgaat gccgaggctg gtgacctcga tgctcatctc ggtgctgaac
     5941 cccttcacca gtggcaccgt gaactcgtgg aatgcctggc tggcctggcg cacagccgca
     6001 tcgggatggc gctgagccac atcggatgca gcagccgaaa cgctggccag cgcacgacaa
     6061 ccttcgatgt gtgcgcgcat ctgcatgagc atgcgcttga cgtcggggtg attgatgatg
     6121 gcggcaccag ccggcagcga cgcatccacc ggacgactct gcacgcgatc ccttgcatag
     6181 gacaccgcgc gctggtacgc gcgctcggca acaccgatgc cctgcatccc gacagagaag
     6241 cgtgcggcat tcatcatgac gaacatgtac tcgaggccgc ggttctcctc gccgatcagt
     6301 tgcgcgatgg cgcccgcacc cacttcgccc tggtcatcac cgaacaccag caccgcggtc
     6361 gggctggccc tgatgccgag cttgtgctcg atcgacgcgc aatagacatc gttgcgcgct
     6421 ccgagcgagc cgtcggcgtt cgccaggaac ttcgggcaca ggaacaggct gatacccttg
     6481 accccaggcg gcgcatcggg agtgcgggcg agcacgaggt ggacgatgtt ctcggccatg
     6541 tcgtgctcgc cgtaggtgat gtagatcttc tggcccgtga tgcggtaggc tccatcgccc
     6601 tgcggcaccg cccgggtccg caccatcgcc agatcggagc ccgcctgcgg ctcggtcagg
     6661 ttcatcgtgc cggtccaggt gccgtcgatc atccggggca ggaagaagct gcgctgttcc
     6721 tggttgccag cggtcaagag cgcctcgatg gccccgtccg tgagcagcgg gcacagcgcg
     6781 aagctcacgc tggcgctgtt gagcatctcc gaacaggcag cagcaatcgt cttgggcagc
     6841 ccctggccgc cgaattcgat cggatgctgc aagccctgcc agcccccttg cgcgtattgc
     6901 cgaaacgcct gcctgaagcc ggcagaggtg ctcacctcgc cactgcgcca caccggggga
     6961 tgcctgtccc cttcggcatt cagcggcgcg accacctcac tgcagtagcg ggcagcttcg
     7021 gtcagcaccg cctcggccgt gtcgatgccg gcatcctcga agcccggcag attcgaaatc
     7081 ccttcgagcc ccgcgagttc cttcatgcag aacagcatgt ccctgactgg cgcgacgtag
     7141 ctcatggaac tccttttcgt cgcccgctgc cggtatcgga tgccagcggg gtgactcgga
     7201 ccggctgctt acagcgcggc caccagcgcc gggacggcga tgaacagatc ggcctcgagc
     7261 ccgtagtcgg cgacactgaa gatcggggct tcaccgtcct tgttgatcgc gacgatcacc
     7321 ttgctgtcct tcatgccggc caggtgctgg atcgcgccgc tgatgccgca ggccacatag
     7381 agctggggcg cgacgatctt gccggtctgg ccgacctgcc agtcgttggg cgcgtaaccg
     7441 gcatccaccg cggcgcgcga ggcgcccatg gccgcgccga gcttgtcggc cagcggcgtc
     7501 atgacttcgg tgaacttctc ggcgctgcca agggcccggc caccactcac cacgatcttg
     7561 gcggcggtga gctcggggcg gtcgttcttg gcgatctcgc tgccg
//
LOCUS       DAOORV010000157         7557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12002_length_7557_cov_8.031058, whole genome shotgun sequence.
ACCESSION   DAOORV010000157 DAOORV010000000
VERSION     DAOORV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12002_length_7557_cov_8.031058"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PKV56_15495"
     CDS             <1..1264
                     /locus_tag="PKV56_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOX69264.1"
                     /translation="LRLLVRQVHLVPHEACDRRPDAVTWPTELIVPWLAIADKLGLAV
                     VKIHGHRGYDRFSPTDDESDRLLFPSLYAWADRSPVHASAILMDDGRLFGRVVTEDGR
                     FEPLQAVQVIGDEIEQFGSGGGAAPVPEHGRRIAQTFGGGTFERLRQLKVGVVGCSGT
                     GSPVIEQLARNCVGALVLVDPDHVEDKNLNRIANTTRADAEQGRLKVDVAARAIAAMG
                     LGTSVQTLASTLFDPVVVRELASCDVLFGCMDTIDGRHLLNKLATFYLIPYFDLGVKI
                     EADGLGGVNQVCGSVHYLQPGGSSLLSRHVYSLEQVRAAGMMRTDPQQYQRLRDEGYL
                     RGVQEDRPAVVQLNTLIASLAVNEFLARLHPYRLDPNADHAVTRISLSHGIFEHEADG
                     EPCPVLARHAGRGDVEPLLDWPELSGGS"
     gene            1261..1617
                     /locus_tag="PKV56_15500"
     CDS             1261..1617
                     /locus_tag="PKV56_15500"
                     /inference="COORDINATES: protein motif:HMM:NF039808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6527 family protein"
                     /protein_id="HOX69265.1"
                     /translation="MSWKLALPLWIRRLTLGTARYRWERVEDTPDEPAPGVVYLIGDG
                     PEAWSAAMVCPCGCEALVSLSLIPTDRPRWKASVSPAGEISLHPSVWRKRGCRSHYSV
                     VRGKIVWARGRARAPD"
     gene            complement(1651..3252)
                     /locus_tag="PKV56_15505"
     CDS             complement(1651..3252)
                     /locus_tag="PKV56_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOX69266.1"
                     /translation="MRRLDAPSRAAVRAVIDAHLAELKVLPGFVGAEPGFPVVDGRIL
                     REPAILVFVSHKRPPTEVLAEDRVPRQLGAYRVAVLQADPWRQLEAKMAAEGSAFDLT
                     ADAGSLSYEGIEGDPIDTEHEIEGSLLCHLGPDAGWPVLKPFLEATQSSLTVAMYDFN
                     AAYIAKTFIDTVRGGGIEAALTWDDSMTAPETEIRGQLRQRLKNRLDGAIIQCGSGRR
                     FASAYHEKVAVRDHASFWLSSGNWSRRSQPDIDPIGDPATAKGMYSAGNREWHLIVDD
                     ESLAQLYERYIRHDLEAAKADLEAGEPGVALGAEEAWRFPDLLVPIESLTAEPGLALA
                     TVEPMAPQALPSVPRRVKVQPLLTPDNYVGRVSSWLASARHSIYLQFAYITYTDKPAD
                     ERFTELLLSLAELSYKPGLDMRIIVGNNGAADKVRRLAEAGFNEAVFRVQGNVHNKGI
                     VVDGKSVLVSSANWSGDGVLRNRDAGLIVHDPEVAGYFSRAFLDDWETRARPTLADNA
                     PVLIAPDDGSTPSGMVRMAWRDYFT"
     gene            complement(3394..3657)
                     /locus_tag="PKV56_15510"
     CDS             complement(3394..3657)
                     /locus_tag="PKV56_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69267.1"
                     /translation="MAIDLSKLRKLETPSVSVQSRRPSEDEKMVVLVKLRQGFARPSY
                     LVPRGEISAALFSTEVPASALQRIESDPAVESVSISKRLPGIL"
     gene            complement(3774..5864)
                     /locus_tag="PKV56_15515"
     CDS             complement(3774..5864)
                     /locus_tag="PKV56_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOX69268.1"
                     /translation="MVDTFLTRTDKAPKDQPPEDRARVQIKRGGRWVGAPGVDAAGLA
                     AAAKAATDDPGVSLPAGGMAAMGLPAPAAGAVAGFAASEAGFLGGLPARLSGIAQADL
                     PSLRRSAGGRPATMDARLMLAIINRQRGKQALSITSAGSNEVAVVARVADAALWRELP
                     DVLPGAQLGVTPDGSAIVTGRVAIDRIEALRAETVVRSLKASQLVRPQLEATTRTMRV
                     RADLLPAGVKPKGGSGVVVGIVDFGGDFAHRNFRKADGRTRLLAIWNQAGVAQANSPF
                     NYGRLYTRPEIDAALLSPDPYAALGYGPRPDTPFERGSHGTHVMDIAAGNGRGSGLAG
                     VAPEADLVFVEASANDIAWQGPDVVKNSFGDSVQMLEAIRFIFDLAGDRPCVVNLSLG
                     TNGGPHDGSSLVEQGIDAMVSEKPNRAVVIAASNSQGDGIHTSGQAPATGLVEDLVWR
                     VRDLVGGEAEVWFAGAKRLELSLIGPDGTVVLDALQPGDTAEIADDQGKLAVFASSRL
                     TEPNNGDNVLGVYLAPGLPGGDWTIRLRSLGGDAVDYHAWVERLDSAQSSFARPVPTH
                     TLGSISTGRLSIVVGSYDAHKASFPISSFSSSGPTRDGREKPEISAPGGFVIAAHSRT
                     GTGVVRKSGTSMAAPAVTGLVALMLAEAARKGVQLSITDIRAKLAAGAEQQPPAAGPW
                     DPRYGAGRASANAI"
     gene            complement(6413..6748)
                     /locus_tag="PKV56_15520"
     CDS             complement(6413..6748)
                     /locus_tag="PKV56_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69269.1"
                     /translation="MALGHTKPTFPRWPFHSQGNAALHNACVQVPDPQSTVALFVALT
                     RAVREAEVHDDVRIRAYDVIDRMAQTVGQTGCRVELEALAALASEHAQMHLALQPFWD
                     DLLALVPKQ"
BASE COUNT         1245 a         2592 c         2488 g         1232 t
ORIGIN      
        1 tttgcggttg ctggttcgcc aggttcacct ggttccccac gaggcctgcg atcggcggcc
       61 ggatgcggtg acgtggccga ccgaactcat cgtgccctgg ctcgccatcg ccgacaagct
      121 tggcctggcc gtcgtgaaga tccacggcca ccggggctat gaccgcttct cgccgacgga
      181 cgatgagtct gaccggctgc tcttcccttc cctgtacgcc tgggccgacc gctcgcccgt
      241 gcacgccagc gcgatcctga tggacgacgg ccggctgttc ggccgcgtcg tgaccgaaga
      301 tggaaggttt gagcccctgc aggctgtcca ggtgatcggc gatgagatcg agcagttcgg
      361 tagcggcggc ggggcggccc ctgtcccgga gcatggccgg cgaatcgccc agaccttcgg
      421 cggcggcacc ttcgagcgcc tacgccagct caaggtcggc gtggttggtt gctcgggcac
      481 cggcagcccg gtgatcgagc agttggcgcg caactgcgtc ggcgcgctgg tgctcgtgga
      541 tccggaccac gtcgaggaca agaacctgaa ccggatcgcc aacaccacgc gggccgatgc
      601 cgaacagggg cggctcaagg tggacgtcgc cgcgcgggcc atcgcggcca tgggcctcgg
      661 aacatcggtc caaacgctcg cctccacgct gttcgaccct gtggtcgtgc gcgagctcgc
      721 cagctgtgac gtgctgttcg gctgcatgga caccatcgac ggccggcact tgctcaacaa
      781 gctggccacg ttctatctga ttccgtactt cgacctcggc gtcaagatcg aagccgacgg
      841 gttgggcggt gtgaaccagg tgtgcggctc ggtgcactac ctgcaacccg gcggatcgag
      901 cctgctgagt cgacatgtgt actcgctcga gcaggtgcgg gcggcgggaa tgatgcgaac
      961 cgatccccag cagtaccagc gcctgcggga tgagggttat cttcgtggcg tccaggaaga
     1021 ccgtcctgcc gtcgttcagc tcaacaccct gatcgccagt ctggcggtca acgaatttct
     1081 ggcgcgtctg catccctacc gcctcgaccc caacgccgac catgccgtga cacggatcag
     1141 cttgagccat gggatattcg agcacgaggc cgacggcgag ccctgccccg tgctcgcgcg
     1201 acatgcggga cgcggcgatg tggagccgct gctggactgg cccgaactga gtggtggctc
     1261 atgagctgga agctcgctct gcctctatgg atccgccggc tcacgctggg caccgcacgc
     1321 tatcgctggg agcgcgtcga ggacaccccg gacgaacctg caccgggcgt cgtctacctc
     1381 atcggcgacg gaccggaagc atggtcggcg gcgatggtct gcccttgcgg ctgcgaggcc
     1441 ttggtgtcgc tgagcctgat cccgaccgac aggccgcgct ggaaggccag cgtcagcccc
     1501 gccggcgaga tctcgctgca cccctcggtg tggcgcaagc gcggctgtcg cagccactac
     1561 agcgtcgtcc gaggcaagat cgtctgggca cgcggccggg cacgcgcgcc ggactgacac
     1621 cgcgagcccg cggcccgatc gggattcgga tcaggtgaag tagtcgcgcc aggccatgcg
     1681 caccatgccg gaaggcgtgc tgccgtcatc cggggcgatc agcacggggg cattgtcggc
     1741 gagcgtcggc ctggcccgtg tctcccaatc gtcgaggaag gcgcggctga agtagcccgc
     1801 gacctcgggg tcgtgcacga tcaagccggc gtcgcggttg cgcagaacgc catccccgga
     1861 ccagttggca ctgctgacca gcacggactt gccgtcgacg acgatgccct tgttgtgcac
     1921 attgccctgc acgcgaaaca ccgcctcgtt gaaaccggcc tccgccagtc gccggacctt
     1981 gtcggcagcg ccgttgttgc cgacgatgat gcgcatgtcc aatcccggct tgtagctgag
     2041 ctccgcgagg ctgagcagaa gctcggtgaa gcgctcgtca gcgggcttgt cggtgtacgt
     2101 gatgtaggcg aactgcaggt agatcgagtg ccgcgcggag gccaaccacg acgagacgcg
     2161 tccgacgtag ttgtcgggcg tcagcagcgg ctgcactttg acgcgccgcg gcaccgatgg
     2221 cagtgcctgc ggcgccatcg gttcaacggt cgccagtgcc agtccgggtt cggcggtcaa
     2281 agattcgatc ggcacgagca ggtcgggaaa gcgccaggcc tcttccgcgc cgagggccac
     2341 gcccggctcc ccggcctcga gatcggcctt ggcggcctcc aggtcatgac ggatgtagcg
     2401 ttcgtacagc tgggccaaag actcatcgtc cacgatgaga tgccactcac ggttcccggc
     2461 ggagtacatg cccttggccg tggcgggatc accgatcgga tcgatgtccg gttggctgcg
     2521 cctgctccag ttgccactgg acagccagaa cgacgcgtgg tcccgaaccg ccaccttctc
     2581 gtggtaggcg gacgcgaagc ggcgtcccga cccgcactgg atgatggcgc cgtccagacg
     2641 gttcttcagg cgctgtcgga gttggccgcg gatctcggtc tccggcgcag tcatgctgtc
     2701 gtcccaggtc agcgctgctt cgatgccacc accgcgaacg gtgtcgatga aggtcttggc
     2761 gatgtaggcc gcattgaagt cgtacatcgc caccgtcaag gaggactggg ttgcctccag
     2821 gaacggcttc aggaccggcc agcccgcgtc cgggcccaga tggcagagca atgagccttc
     2881 gatctcatgc tcggtgtcga tcggatcacc ctcgatgccc tcgtacgaca ggctgccggc
     2941 gtccgcggtc aggtcgaacg cggagccttc ggcggccatc ttagcttcca gctgccgcca
     3001 aggatcggcc tgcaggaccg ccacacgata ggcccccagt tgacgtggca cgcggtcctc
     3061 ggcgaggact tcggtcgggg gtctcttgtg ggacacgaag accaggatcg ccggctcgcg
     3121 caggatgcgt ccgtcgacga ccggaaagcc tggctccgca cccacgaatc ccggaaggac
     3181 cttcaactca gccagatggg cgtcgatgac ggccctgacg gcggccctgg acggggcatc
     3241 aagtcgtcgc atgcgcatgt ccttccgagt tcatgtcacc aaccggcccc agattcaggc
     3301 gctcgttggc atggcgaagc agaagaagca cgaccgccgc ccgcgcccgc agtcgtcgcg
     3361 aacggctgcc gacggcagcc gatgatcgac gtctcagaga atgccgggaa gacgcttgga
     3421 aatcgagaca gactcgacgg ccgggtcgga ctcgatgcgc tgcagcgcag acgcaggcac
     3481 ctcggtcgag aacaacgcag ccgagatctc gccgcgcggg acgagatagc tgggccgcgc
     3541 aaacccctgt ctcagcttca cgagcacgac catcttctcg tcctcggaag gccggcgcga
     3601 ctgcacggac accgagggcg tctcgagttt gcgcagcttc gaaaggtcga tggccatggt
     3661 cgatcaagtg ggtccgtgcc ggacggaaac aagcgtccca cccggcgtcc gcaggcgggg
     3721 ctgcttcagc atagcgcaaa ccccagcatg cgtccacgat cgctggcccg gtgtcagatc
     3781 gcattcgccg acgcgcgccc ggcgccatag cggggatccc acggtccagc cgccggcggc
     3841 tgctgttccg caccggcggc cagtttggcg cggatgtcgg tgatcgacag ttggacgccc
     3901 tttcgtgccg cctcggccag catgagcgca acgaggccgg tgaccgccgg ggccgccatg
     3961 ctggttccag acttcctgac aacgccggtc ccggtgcgcg agtgcgccgc gatcacgaat
     4021 ccgcccggcg ccgagatctc cggcttctcg cgtccgtctc gcgtgggccc cgaggaggag
     4081 aagctggaga tcgggaacga agccttgtgc gcgtcatacg agcccacgac gatcgacagg
     4141 cgtcccgtcg aaatcgaccc gagcgtgtgt gtgggcacgg gccgggcgaa cgacgactgc
     4201 gccgagtcca gccgctcgac ccacgcgtgg tagtcgacgg cgtcgccgcc cagcgagcgc
     4261 agccggatcg tccagtcgcc accgggcagg cccggcgcca gatagacgcc cagcacgttg
     4321 tcgccgttgt tcggctcggt cagccgactg ctcgcgaaga cggccagctt gccctggtca
     4381 tccgcgattt ccgccgtatc cccaggctgg agggcatcca gcacgacggt tccgtcgggc
     4441 ccgatcaggc tgagctcgag ccgcttggcg cccgcaaacc agacctcggc ttcgccgccg
     4501 accagatctc gaacccgcca gacgaggtcc tcgacgaggc cggtcgcggg agcctggccc
     4561 gaggtgtgga tgccgtcgcc ctgcgagttg ctggccgcga tcaccacggc acggttaggc
     4621 ttttcgctga ccatggcatc gatgccctgc tcgaccagcg aactgccgtc gtgcgggccg
     4681 ccgttggtcc cgaggctcag attcacgacg caaggcctgt cgccagcaag gtcgaagatg
     4741 aaccggatgg cctccagcat ctggaccgag tccccgaacg agttcttcac gacgtcgggg
     4801 ccctgccatg cgatgtcgtt cgcggatgcc tcgacgaaca ccagatcggc ttccggtgcc
     4861 acacccgcca gaccacttcc gcgcccgttg ccggccgcga tgtccatcac gtgcgtgcca
     4921 tgcgaaccgc gctcgaaggg tgtgtcgggg cggggcccat aaccgagcgc ggcgtacggg
     4981 tccggtgaca gcagcgccgc atcgatctcc ggccgcgtgt acaggcggcc gtagttgaag
     5041 gggctgttgg cctgggcaac gccggcctgg ttccagatcg ccagcagccg ggtcctcccg
     5101 tccgccttcc tgaagttgcg gtgggcgaag tcgccgccga agtcgacgat cccgaccacc
     5161 acgcctgagc cacccttggg cttgacgccc gccggcagga ggtccgcgcg gacccgcatg
     5221 gtccgggttg tcgcctccaa ctggggccgg acgagttgcg aggccttcag gctgcggacc
     5281 acggtctctg cgcgcaaagc ctcgatgcga tcgatggcca ctcggccggt cacgatggct
     5341 gagccatcgg gcgtcacgcc gagctgggct ccaggaagga cgtccggcaa ctcgcgccac
     5401 agcgctgcat ccgcgacgcg ggcgacgacc gccacttcgt tgctgcctgc cgaggtgatc
     5461 gacagcgcct gcttgcctcg ctggcggttg atgatggcca gcatcaaccg cgcgtccatc
     5521 gtggcaggtc ggccaccggc cgaccgacgc aggcttggca gatcggcctg ggcgatgccc
     5581 gagagtctgg cgggcaagcc gccgagaaat ccggcctcac ttgcagcgaa acccgccaca
     5641 gcgccggcgg ccggcgctgg caaacccatc gcggccatac cgcctgccgg cagcgacact
     5701 cctggatcat cggtcgcggc cttggccgcc gcagcaagtc cagcggcgtc gacgcccggc
     5761 gcgccgaccc atcggccgcc cctcttgatc tgcactctgg cgcgatcttc gggcggctgg
     5821 tccttgggcg ccttgtcggt tctggtcagg aacgtgtcaa ccacggagcg gctcaagcca
     5881 tcaaggacga cccgagcgtc attcccgaga gggaacttga gcgcgaacga tgctgccggc
     5941 gcccgggccg cgagctcagg cccacctctg aaagaggcgc tcgccttttt ggcgacaggc
     6001 ttggctggcg cggcgcgttt ggcggctttg ggctttggcg cggccggctt cttcgcggcc
     6061 ttgaggacag ccttgcccga acgtgccttt tgcttggtcg ccatttggcc ttcctcctac
     6121 ccgcttctac gctgcggcgt cagtgtgcgt cgcaaggaac gggtcgaacc gagcgacgct
     6181 gtcgtttgcc tcaggctgac gctcctcgcg tcgttgcgca tccttcgatc ggatgacgca
     6241 ccacattctg tggtctcccg ctacgaatga tccatggtcg aacggccggt cgagacgggc
     6301 acgttggcga tgccgtcgtc aagccctacc ggcaggcgcg caagcgacca aacccctgtg
     6361 aacggcctgc ggccggtcgg gcccatttga caggtttcgg tgccgcgtgg gtctactgct
     6421 ttgggaccag cgcgagcaga tcatcccaga acggctgaag cgccaaatgc atctgagcgt
     6481 gttcggaggc caaggcggcc agcgcctcga gttccacccg gcaaccggtc tggccgaccg
     6541 tctgcgccat gcgatcaatg acgtcgtagg cccggatccg aacatcgtca tgcacctcag
     6601 cctcgcgcac ggcgcgggtc agcgctacga agagggccac tgtggattga ggatccggta
     6661 cctgcacgca cgcattgtgc aatgcagcat ttccctgtga atgaaatggc cagcgcggga
     6721 atgttggctt ggtatggccg agtgccactc tcactcggct ccgtcaacaa gacaaacttg
     6781 cataccatcc accaccagtg gcgcacagag gcccgccgtc acggtggagg ggcatcaggt
     6841 actgggcgcc aagtcgccgc aatcgtcgct tcaagcgccg ctcggaaccc ggtcttgacc
     6901 tgctctggcg cccgtcgaac atactcgctg gcacgcacca gcgcttcccc ggagagagtg
     6961 ccgaggtagg acgccgccgc cgcggcgtcc ttcggcccgg cgccatcggc tccgccaaac
     7021 ctcggacgca gggcagtcca aatcacggcg tcgaagcccc ggtctccgcc ccactgggcg
     7081 atctgcgcag cgccagaggt gtgtttcggc atcggccctg gccacaggcc gatcgcatgg
     7141 atggctgttc cctcgcggcc ggccagcgcg gcttgcgacg cttcccccgt gaggtaatcc
     7201 aactcgcacc acaaggccgg gacgggcgcc gccccaggcg tcaacaccaa cgtcagtctt
     7261 ccatcgcgag agacccgagc gaactcgaga ggcaaggtcg gaccatcgac gtgccaatcg
     7321 ccgacacagc gcagaacacc ggggtcccag accaaagagc cccatccgat acacacgatc
     7381 ctcacgctct gctcccccat tgccaccagc catccgacat cgtacgggct cgtgtcggcc
     7441 gcgccagcac ggtcgcccga gcgacttcgc ggccgtctcc ccaacgtgta ccgcccacgc
     7501 acgagcgccg tcaggttcga gaagcgaatg gcgccgccta cagccgacgt tgactag
//
LOCUS       DAOORV010000158         7520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12096_length_7520_cov_6.638044, whole genome shotgun sequence.
ACCESSION   DAOORV010000158 DAOORV010000000
VERSION     DAOORV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12096_length_7520_cov_6.638044"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2659
                     /locus_tag="PKV56_15525"
     CDS             <1..2659
                     /locus_tag="PKV56_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69270.1"
                     /translation="LRPPRLVGRAAELRVLVSAVAEGRVVLLSGEAGLGKSRLIAALC
                     ESSSATTAVLSVAARVGDDMAPFALAARWLRALLQRPGMEPSDAQRADLAGVLPELGP
                     AAPRSPAADRARQAQAIEALLVTAAGQGLRACALDDLQYADAASVELLHALAGCDSVG
                     WVLALRPDGLGEAQRALLSAHRDSTRATALALQPLAGEDVEALLDSLGLPGVGGSEQA
                     QALKRRTGGNPMYLLETIKAAWSTHGGALAPMAGRPEAAVVWPQADNVLRLIQQRLSR
                     LSLLATQVVRCAAIAGQDLSAALVAQVLGLRPLDLADAWAELESAQVLRDGGGFAHDL
                     IAEAARSSLAHAIARPLHGEVAAFLEQHGGEPARVAEHWLEAGEPLRAVPHLSAAARR
                     ARGAGRLLEAAGLYERAAHALQDAGQRGAAFDAFFAAQDLLSESTFVQRLESLIDALD
                     ALADNDDGRRALAACARIVLLGEQGRHDDAVQLAMQALPWAERAGLPDVQAELHWDLA
                     ALRWMRRELAEATRHAEQALACLGRADPAHAYLARAGRSLTASRSRVLNALGLLMAAA
                     GRLAEANARLEENWHLARAERDVATARNIAAGLAMNSLDQGQLAPALHWSEQAMADDE
                     RLDVDPSTRLTALTNRTAVLAISGDLGGAVALADRVAALCAQGTARAIPAALRRMHWL
                     ECELGRRDLARKGLEALLERPNLLAIDRLAIEATLLHAGGPPHSADLLDRIAGLDDFP
                     MRARLLVLARPGCDATAILPLLSATAATARDCGAMGLWLDLQLARLAALRDAGRSAEA
                     AQSALALWTRLDQGLVGMGLFDRQASAVVMALPDSHASLAGTVRLRISAWRDQAASTL
                     PEPWRENYLARSPSMLGDSPSARGPRLAP"
     gene            2753..3091
                     /locus_tag="PKV56_15530"
     CDS             2753..3091
                     /locus_tag="PKV56_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOX69271.1"
                     /translation="MNRSPQPCGKMDQAIGEARRVGPEASATLPVQLAAAWLPYLPQI
                     WVDDWVVNLDANDEPAVVSEPGRQFLWVLSREPGLLSADRASLNARLIQWQSGPCRTC
                     SSWRFSGSDR"
     gene            3088..3423
                     /locus_tag="PKV56_15535"
     CDS             3088..3423
                     /locus_tag="PKV56_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69272.1"
                     /translation="MTLFGSVGAPTRLPPAADIGFLLAVAIVIGCGYWGILTSRTSID
                     GQHIAQTWLWHEKVAIVDITQVKLVRVQRLDLLVVPLLVVRAGYGLTTIQTGGTTVHS
                     QFRLLPHGG"
     gene            4215..5795
                     /locus_tag="PKV56_15540"
     CDS             4215..5795
                     /locus_tag="PKV56_15540"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX69273.1"
                     /translation="MIVRLLVATLVCVGSWGHAWAGYAEGDAAFQRKDYATAFREFSS
                     AAAQGNAQAQGALGLLYLRGLGVAEDLRQAEQWLRKSANQGQPGSQWMLGTMYDSGVG
                     LPKDDEQAVFWFRIAALNGNDRAQLRLGAKLAEGEGVAKDEEEAAIWYGRAADQGNAD
                     AQHNLGLIFRAGRGVPKDDQKSLFWFRKAAEQGVVSSQGMLGLLYSDGVVVPKDDQQS
                     AFWYRQAAEQGEWRAQNLLAGKYLMGEGVPRDAQQAYFWLLLAASKGDANAASLRDSV
                     ERTLSPRQRAEAQAAAREWKPKIRAQVEPFKSVAGTGATTTPVPVRLETTRSTGSGFF
                     ATSDRIVTNHHVTDGCTRLTVLGQPARLIASEPRSDLALVEVDGGSPNIASIRVGKVA
                     LGELTAVAGFPLNGLLSGLNVTTGIVSSLSGIRGDTRLLQITAPVQAGNSGGPLLDAS
                     GNVIGVVVSKLDAAWVAKVAGDVPQNVNFAIRSNVLASFLDANGVDYRSSQAGSKLST
                     QQVASRAQAFTVLVECWK"
     gene            5835..6071
                     /locus_tag="PKV56_15545"
     CDS             5835..6071
                     /locus_tag="PKV56_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69274.1"
                     /translation="MQDNIEAATFGMFHAVQVAIRALIATHPDPSSLVQAFAAEHLES
                     MSMLLASPFPDASIDAYKDFLRGCAPNADDWLEA"
     gene            6102..6848
                     /locus_tag="PKV56_15550"
     CDS             6102..6848
                     /locus_tag="PKV56_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69275.1"
                     /translation="MWILLLGYLYMAGSFLVACRLGWYMAFRLDGFDWRYASRAVWRS
                     FAIWLLFWPLLVLRWREVVLEPRVALDADRYAAQEARFWDCPPPCGAYVRFQQAASLR
                     QASACGEFVFRSDDVADWLTQRRVVDPDRLPEGDIQRWINTRDGQIIEPTDVPSIASR
                     FEFIADEMVRMGFGEVRCRRCNAQIPHEALIYSDDDGNQMSWNYNRILCPQRHPLLLV
                     GMVHVSIDSDALVSALGDGSGEGSASGGQE"
BASE COUNT         1197 a         2430 c         2612 g         1281 t
ORIGIN      
        1 gctgcggccg ccgcgcctgg tggggcgcgc ggccgagttg cgcgtgctgg tgagcgcggt
       61 ggcagaaggc cgggtcgtgc tgctgtcggg cgaggcgggc ctgggcaagt cgcggctgat
      121 cgccgcgctg tgcgagtctt cgtctgccac cacggcggtg ctgtcggtcg ccgcgcgtgt
      181 cggcgacgac atggcaccgt tcgcgctggc ggcacgttgg ctgcgcgcgt tgctgcagcg
      241 gcccggcatg gagcccagcg atgcgcagcg cgccgacctg gccggcgttc tgccggagct
      301 cggcccggcg gcgccgcgct cgcccgcggc cgaccgcgca cggcaggccc aggccatcga
      361 ggccttgctg gtgacggcgg caggccaggg gctgcgtgcc tgcgccctcg acgacctcca
      421 gtacgccgat gcggccagtg tcgagctgct gcatgcgctg gccgggtgcg acagcgtcgg
      481 ctgggtgctg gccctgcggc ccgatgggct cggtgaggca cagcgcgcgc tgctgtcggc
      541 ccaccgcgac tcgacacggg ccacggcgct ggcgctgcag cctctggctg gcgaggacgt
      601 ggaggcgttg ctcgactcgc tgggcctgcc cggcgtgggc ggcagcgaac aggcgcaggc
      661 gctgaagcgc cgcaccggcg gaaacccgat gtacctgctc gagaccatca aggccgcgtg
      721 gtcgacgcac ggcggcgcgc tggcgccgat ggccggcagg cccgaggcgg cggtcgtctg
      781 gccgcaggcc gacaacgtgc tgaggctgat ccagcaacgg ctgtcgcgcc tgagcctgct
      841 ggcgacccag gtcgtgcgct gcgcggcgat cgcaggccag gacctgagcg ccgcactggt
      901 ggcgcaggtg ctggggctgc gcccgctcga cctcgctgac gcctgggcgg agctggagtc
      961 tgcccaagtg ctgcgcgacg gcggcggctt cgcgcacgac ctcattgccg aggccgcgcg
     1021 cagcagcctg gcgcacgcca ttgcccgccc gctgcacggc gaggttgcgg ccttcctcga
     1081 acagcatggc ggcgagccgg cgcgtgtggc cgaacactgg ctcgaggccg gcgagccgct
     1141 gcgtgccgtt ccgcatctga gcgccgccgc tcggcgcgcg cgcggggccg gccggctgct
     1201 cgaagccgcc ggactgtacg agcgcgcggc gcacgcgctg caggatgccg gccagcgcgg
     1261 ggccgccttc gatgccttct ttgccgccca ggatctgctg agcgaatcga ccttcgtgca
     1321 gcgcctcgag agcctgatcg atgcgctcga tgcgctggcc gacaacgacg acggccgccg
     1381 cgcgctggcg gcctgcgcgc gcatcgtgct gctcggcgag caggggcggc acgacgacgc
     1441 cgtgcagctc gcgatgcagg cgctgccttg ggcggagcgc gccggcctgc ccgacgtgca
     1501 ggccgagctg cactgggatc tcgccgcact gcgctggatg cgccgcgaac tcgccgaggc
     1561 cacgcgacac gccgagcagg cgctggcgtg cctggggcgg gccgatccgg cgcatgccta
     1621 cctggcgcgg gcgggtcgat cgctcacggc cagccgcagc cgcgtgctga acgcgctcgg
     1681 cctgctgatg gccgccgccg gacggctggc cgaagcgaac gcgcgtctgg aagagaactg
     1741 gcatctggcg cgcgcggagc gcgatgtcgc cacggcccgc aacatcgcgg ccggcctggc
     1801 catgaactcg ctcgatcagg gccagctcgc acccgcgctg cactggagcg aacaggccat
     1861 ggccgacgat gagcggctcg acgtcgatcc ctccacgcgg ctgaccgcct tgacgaaccg
     1921 gaccgccgtg ctcgcgatca gtggcgatct cggcggcgca gtcgcgcttg ccgaccgcgt
     1981 ggccgctctc tgtgcgcaag gaacggcccg ggccatcccc gccgcattgc gccgcatgca
     2041 ctggctggag tgcgagctgg gccggcgcga tctggcccgc aaggggctgg aggcgctgct
     2101 cgaacggccg aacctgctgg ccatcgaccg tctcgccatc gaggccaccc tgctgcacgc
     2161 gggcgggccg ccgcacagcg ccgatctgct cgaccgcatc gccggcctcg acgacttccc
     2221 gatgcgcgcg cggctgctcg tgctggctcg ccccggctgc gacgcgacgg cgatcctgcc
     2281 gctgctcagc gcgacggcag cgaccgcacg cgactgcggc gcgatggggc tctggctgga
     2341 cctgcaactc gctcgattgg ccgccctgcg cgacgccggc cgaagcgccg aagcggcgca
     2401 gagcgccttg gcgctgtgga cccggctcga tcagggactg gtgggcatgg gtctgttcga
     2461 ccgccaggcg tccgcggtcg tcatggcgct gcccgacagc cacgcctcgc tggccgggac
     2521 ggtacggctg cgaatctccg cctggcgaga ccaagccgca tccaccttgc ccgagccctg
     2581 gcgagagaac tacctggctc gctcgccctc gatgctcgga gactcgccat cagcccgcgg
     2641 accgaggctg gccccttgag aactggctcg ctacccgaac cgctttcggc agcaatgttc
     2701 atgagtggcc accgccagct gctccccgct gccgacgtgc cgcgtgcagg tggtgaaccg
     2761 cagtccgcag ccgtgcggca agatggatca agctatcggc gaagctcgcc gggtgggccc
     2821 tgaagcttcg gcgacgctgc cggtgcagct tgccgcagcg tggctgccct acctgccgca
     2881 gatctgggtc gacgactggg tggtcaatct ggatgcgaat gacgaacccg cagtggtcag
     2941 cgaacccggg cggcaatttc tctgggtgct ctcgcgcgag cccggcctgc tatcggcgga
     3001 ccgggcgtcg ctgaacgccc ggcttatcca gtggcagtca gggccctgtc gaacgtgctc
     3061 gtcgtggcgc ttctcgggtt cggatcgatg acgctgttcg gctcggtggg tgccccgacg
     3121 agactcccgc ccgccgcaga catcggattc ctgctcgccg tggccatcgt catcggatgc
     3181 ggctactggg gcatcctgac gagccgaacg agcatcgacg gccagcacat cgcccagacc
     3241 tggctttggc atgagaaggt cgccattgtc gacatcaccc aggtcaagct ggtgagggtg
     3301 caacgccttg atttgctggt ggttccactg ttggtcgtac gagccggcta cggtctgacg
     3361 accatccaga cgggtggcac gaccgtgcat tcacagttca gactgttgcc gcacggcggc
     3421 tgagggttcg tctgcagatg cagccagtcc gcttgaaccc gattcacggc agcgccgcgg
     3481 ccggactccg caagagttgc actttccgac agaggtacgt ttcccggggt gcttctcaag
     3541 cggcagaccg ccggtgcctg ctgtccagct ggagcctagc tgatgtccgc attcctgctc
     3601 agcgatagtc tgctcagggt cggcacttga ccgacccctt tccgagttga tcgccgtaga
     3661 gctgccgttg gaggcacgcc gcccggaagc ggctcttccg ctacgagtgg actcgaccct
     3721 gaagcgacat ccgcgaccct gatcccgtcg ccccaaagct gccggtcgga ctcgcaccca
     3781 ggcacccgaa ggaggccccg cagcggaggg cacacatcgt taggcactag tcggagcgat
     3841 tgaggcgtgg cgcgcggctt gagaccgtcc gaggtcacca cgtagggagc cacgcgtgcg
     3901 cgccatcgag cggcagcccc agcgggcgtt gagtggcgcc ccggtccact acggggcgct
     3961 accggcccga atagaccacg ccatcttgtg cggcagcacg ccgccaagtt catcgctcac
     4021 acccaggcca gtaccggcgc cgccccggca atcgcgcgcg acaatgcttg ccaaaggcat
     4081 cggaattggg ccacaggcta gatgaggcag acaggtgcgt tgctcgggac tacttcacgc
     4141 gccatgtagg tactgtctct tcgtcagtac cgagcgatca gtagactggg gtgggaagtg
     4201 aggtgggagg cgtcatgatt gtgagattgc ttgtcgcaac gctggtctgt gttggctctt
     4261 ggggtcacgc ttgggctggg tacgccgaag gcgatgccgc ctttcagcgg aaggactatg
     4321 caactgcgtt tcgggagttt tcgtccgcgg ctgcgcaggg aaacgcccag gcgcagggcg
     4381 ctctaggatt gctgtacctt cggggtcttg gcgtggccga agatctacgc caggccgagc
     4441 agtggctccg aaagagcgct aaccaaggtc aaccaggttc gcaatggatg ctgggcacca
     4501 tgtatgacag cggcgtcggg ctacccaagg acgatgaaca ggcggtcttc tggttccgca
     4561 ttgccgctct gaacggaaat gaccgtgccc aactgaggct cggcgcgaag ctcgcggaag
     4621 gcgagggcgt tgcaaaggac gaggaggagg cagcaatctg gtacggcagg gccgctgacc
     4681 aaggcaacgc agatgcccag cacaaccttg ggttgatatt tcgcgctggc cgtggtgtgc
     4741 caaaggacga tcagaagtcg ctcttctggt ttcgcaaggc cgcggaacaa ggagtcgtca
     4801 gttcgcaggg aatgctcggc ttactgtatt cggatggtgt agttgtgccg aaggacgatc
     4861 agcaatccgc gttctggtac aggcaggcgg cggagcaagg cgaatggcgc gctcaaaatc
     4921 ttcttgctgg gaagtatttg atgggcgaag gcgtgccaag ggacgcgcag caagcgtact
     4981 tctggttgtt gctcgcagca tcgaagggcg atgccaacgc tgcgagtttg cgagactctg
     5041 ttgagcgaac cctctcacca cgacagcgcg ccgaagctca agccgccgca cgcgagtgga
     5101 agccgaagat tcgtgctcag gttgagccct tcaagtcagt cgctggaact ggcgccacca
     5161 cgactccggt gcctgtgcgt ctagagacga cgcgatcaac aggctctggg ttcttcgcca
     5221 cgagcgaccg catcgttacc aatcatcacg tgaccgatgg atgcactcgc ttgacagtgc
     5281 ttggccagcc cgcccggctg atcgcgagtg agccacggag cgacttggca ctcgtagagg
     5341 tggatggtgg ctcaccgaac atcgcgagca ttcgggttgg gaaggtcgcg ctgggtgagc
     5401 ttaccgcagt tgcaggcttt cccttgaatg gccttctatc cggcctcaac gtgacgaccg
     5461 ggatcgtgag cagcctgtcg ggcattcgcg gcgatacgcg cctgctgcag atcacggcac
     5521 cggttcaggc cggaaatagc ggcgggccgc ttctcgatgc gagcggcaat gtcatcggcg
     5581 tggtggtgag caagctggat gccgcgtggg ttgcgaaagt cgctggcgac gttccacaga
     5641 atgtgaactt cgcgattaga tccaacgtgc tcgcatcctt tctcgacgcc aacggagtcg
     5701 actataggtc ttctcaggcc ggctcgaaac tttcaacgca gcaggtcgca agtagagcgc
     5761 aggccttcac cgtgttggtc gagtgttgga agtagcgcgg ctggtaccgg agttcaaact
     5821 gagcagagga agacatgcaa gacaacatcg aagccgcgac gttcggcatg tttcacgcgg
     5881 tccaggtcgc gatccgtgca ctcattgcaa cgcacccgga cccaagttcg ctagtgcagg
     5941 catttgccgc cgagcatctc gagtcgatgt caatgctcct cgcttcgccc tttcccgatg
     6001 ccagcatcga tgcctacaaa gactttctgc gcggatgcgc acccaacgcc gacgactggc
     6061 ttgaggccta gcactgggca agcgaagcca aggcgcctgc aatgtggatc ctcctgcttg
     6121 gctatttata catggccggt agcttcttgg tcgcgtgccg cctcgggtgg tacatggcct
     6181 ttcgactcga cgggttcgac tggcggtatg cgagccgcgc tgtctggagg tcattcgcaa
     6241 tctggctcct gttctggccg ttgcttgtac ttcgatggcg agaggtggtg ctcgaaccgc
     6301 gggtagcgct cgatgctgac cgatatgctg cgcaggaggc taggttctgg gattgcccgc
     6361 caccctgtgg ggcatacgtc aggtttcaac aggccgcatc gctgcgccag gcctcggcgt
     6421 gcggggagtt tgtatttcgt tcggacgacg ttgcagactg gctgacccag aggcgggtgg
     6481 ttgacccgga tcgattgccc gaaggtgata ttcagcgatg gatcaacacg cgcgacggcc
     6541 agatcatcga gccgaccgac gtgccatcca tagcctcgcg cttcgagttc attgccgatg
     6601 aaatggtgcg catgggtttc ggcgaagtcc gctgccgtcg gtgcaacgcg cagattccgc
     6661 atgaggcgct catctactcg gacgacgatg ggaatcagat gagttggaac tacaaccgca
     6721 tcctctgtcc gcagcgccac ccgttgctct tggtggggat ggtccacgtg agcattgaca
     6781 gcgacgcttt ggtgagcgca ctgggcgacg gctcgggcga ggggtctgca tcgggcgggc
     6841 aggagtagcg gcgtagcggc tccagtcacg ggggaccaat ggaggagcaa atcgatgctg
     6901 gcaggccgag gacggcctgg ttccgcatgc cgcgttcatc acccgctggg acgatgacgc
     6961 accgacgcac cgatctcggc cgtggtaatc acgcccggtc cgggcggcag cgagccgcgc
     7021 agcgtcaacc tgcgctcgcc gcaggcacgg gattagccct gaactcgccc cccgtcggcg
     7081 tgcgctttcg gcctcggtaa agaggcccgc ggcgtgcagc ggcaccgggc ggtgcagcgt
     7141 ggcttgttcg aggcagtggc gttcgtagtg aaccggcgcg ccatcctgcg ccctttgggg
     7201 ctgccagagg tggtgacctc gcacggtctc aaaccgcgcg cggtgcctca atgcacccag
     7261 tgccgcctgg tgcccacctt ggcacggcct cgttctggaa acaacctcgc ggtcgccgac
     7321 gaggggctgt cggcgagatg attggaagct gcctgtcgcc aacgtcagtt gatggccggg
     7381 cagagcctgc gcttaaattg gattcggcgc cgtaaacccg acgttcgccg attcgcccac
     7441 gcggagactg gaagctcagc ggccaggacc gcttgtggcc gggagcggca ggaccaatcg
     7501 cttggttttc tgagacgccc
//
LOCUS       DAOORV010000159         7497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12138_length_7497_cov_6.091642, whole genome shotgun sequence.
ACCESSION   DAOORV010000159 DAOORV010000000
VERSION     DAOORV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_7497_cov_6.091642"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..1439
                     /locus_tag="PKV56_15555"
     CDS             405..1439
                     /locus_tag="PKV56_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOX69276.1"
                     /translation="MTRSPVAHSASAPSPHPRRAFVIGGTGTIGRATVRALVGRGHEV
                     VCLVRPQSGVGGALTRDDSARLLAGATVRLGDVSDPLSLARDGFCGEHFDVLVSCLAS
                     RTGAPKDAWAIDHEAHRHALTAAREAGVSHVVLLSAICVQKPLLAFQHAKLAFEKVLI
                     ESGLTYSIVRPTAFFKSLSGQVDRVKAGKPFLVFGDGTLTACKPISDDDLGRYLAGCL
                     DDESRHNRVLPIGGPGGAITPRQQGEHLFALLGRPPRFKQVPVALLDVIIGVLGTLGR
                     VVPALAAKAELARIGRYYATESMLVFDRSTGRYDADATPSTGTETLFDFYAGLVRGDI
                     VPERGDHAVF"
     gene            complement(1447..2586)
                     /locus_tag="PKV56_15560"
     CDS             complement(1447..2586)
                     /locus_tag="PKV56_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HOX69277.1"
                     /translation="MNKTFHIAIALTGLAIAAQATAQVTFYESSDFRGRTFTTAQPVR
                     NFSEFGFNDRASSAVVSGNRSDRWEVCEDGQFRGRCVTLRPGQYPSLSAMGLNDRVSS
                     ARIVDRNARVDPDRYAPVPPPAQTSQVVFYENERFQGRTYTTERQVRNFRRTEFNDRA
                     SSAVVRGNPWDRWEVCEDGNFRGRCVVLRPGQYPSLSAMGLNDRVSSTRVVARGAQVD
                     DDRYAPLPMVSRDYGRRRNERLYEAEITSVRAVVEESGERCWVEQEQVTRESGGANVP
                     GALIGAVIGGILGHQVGGGTGKNVATGVGIVGGALLGGNVGRSDGDQQQAATRNVQRC
                     SSAPGQGRPAYWDVTYDFRGEEHRVQLTTPPGRTISVNNRGEPRG"
     gene            complement(2760..4475)
                     /locus_tag="PKV56_15565"
     CDS             complement(2760..4475)
                     /locus_tag="PKV56_15565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX69278.1"
                     /translation="MKLGIARKLALLLTLVGALASGLTGLYAYQSSRQLLIESAQDKL
                     LTSTQVLARRIALSRQEITRNLGVLARHPASKSVLQSGDAAQAGQLSTLFCLLMAENP
                     SYFQIRLISASDHGLERVRVDRQGDALLEVKDDELQEKGHYPYVAETLKLQAGEFYLS
                     EIAVNHERGTYAGLDEPALQLATPVTDAGGTAIGVIVVNIDLNGMFSLLAADLPASFR
                     LFLANDKGDILIHPDARRTFGFDKGRRVLIEEEFPPTRDVVEGRVAHVVFDAGEPSGS
                     DVPVVAAFISQLVEIPSHEQRLILGLAQPRAEVVAQAHQLGIAILQIVLGLCLGCLLL
                     AALLARAFTRPINILESAAQGFASRHPSAALPVERQDEIGALARSFQKMQDQITKQLE
                     DLQNNQQELEHMAQHDMLTGLPNRRLFVSRLDKAMAHARRYGGEASVLFIDLDDFKNV
                     NDRCGHDTGDIVLKAIAQRLLTMMREGDTIARIGGDEFVALLGASTSHDQLVAVAEKL
                     LAGIREPIDINGVELKVGASIGISRYPQDGQTVSELLATADNAMYTVKARGHGGFRFS
                     SKVAD"
     gene            complement(4472..5521)
                     /locus_tag="PKV56_15570"
     CDS             complement(4472..5521)
                     /locus_tag="PKV56_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX69279.1"
                     /translation="MPIPHPNRRQFCGALAAAVPGWARAAETLRVLAWPGYADADFVR
                     SFEQKHGSRVEVTLIDSDLDLWTKITANSAQDFDVFAVNTAELQRYIRKGLVVGIAAD
                     SIPNLARQRPRFRDLAAIPGIVRNGTVYAIPYTYSEMGLIYDRQRMASAPDSIEALWD
                     ERYHGEVIAYNGGTHNFSLAALSLGLKSPFRLDDKDWPRAVDRLIALRRNVTGFYTRP
                     DESVGLFKRRKAALMFANYGKQQLQMCKAAGLDVGYALPREGALAWLDCWVVTRGARN
                     PKLAAAWINHLLEDAPSQALVTRQGLGSTTSAESAGEAGEKLRWLQPVESEEHRNQLW
                     GRILSGDRASKVLAS"
     gene            5846..6988
                     /locus_tag="PKV56_15575"
     CDS             5846..6988
                     /locus_tag="PKV56_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOX69280.1"
                     /translation="MTQRTILIVDDNPENLTVLGELLCERYTVRAANSGARALQLAMQ
                     TPLPDLILLDIMMPGMSGYEVLERLQDTPKLRDIPVIFTTAMNAEDDEQRGIALGAVD
                     YITKPLKPTVVLARVQAHLELKLARDRLQQINESLEAEIGRRMRENLTIQDVTIRALA
                     RLAETRDNETGNHILRTQRYVHALTERASCHPRFAGELDEQSVALIAKSAPLHDIGKV
                     GIPDCVLLKPGKLTAEEWDIMQTHAAIGADAISRAEADTCEPVKFLEFAKQVARHHHE
                     RWDGGGYPDGLAGDAIPLAARLMAVADVFDALISRRVYKKAFTLDEARHIMASQGGKH
                     FDPDLLQLFLDGFDEFCEIARRHPDSPEPAESVAETAPAAIAVAAT"
     gene            7026..>7497
                     /locus_tag="PKV56_15580"
     CDS             7026..>7497
                     /locus_tag="PKV56_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69281.1"
                     /translation="MAPFDKREFQVDLLRHGALRIALLYALFGALWILGSDWLLHKLV
                     SDPSWVVQIGAIKGWFFVGVTAWMLYLLIRRIPSGAHPSKRVSKAVPAQRAGWALLYV
                     SIVALTLVALRFDYQHQHELERTTLHTLAELQARLVAHGYEDRAVQARFISSSPH"
BASE COUNT         1183 a         2533 c         2483 g         1298 t
ORIGIN      
        1 caccaccgaa acctatctca attccgcctt gtacgaaatc atcgggacgc cgcaaacccg
       61 tgtcgtaccc gaaccggcca ccggattcct ggtgggcatc gccgggttga tgggctggtt
      121 cgcgaccgtt cggcggcggc gcaacagggc ctgagggccc tcagcgaacc ccgtccgact
      181 cggtcgacgg gggcctgtcg tgcggttccc gggatcgctg attcccggca acgcctttgc
      241 gatctgcccc ggttctctgc acttgatggc ctgctgcgct gcaggccttc agtcacccgt
      301 gttgtgcaaa cgttcggccg gtaacatgcg gcgcgagcac gtctggtcgg tggtgcaacc
      361 ggacccgtgc gcaatcgaat ggcgctcatc ggcttccgca gatcatgact cgctcgcccg
      421 ttgctcattc agcatccgcg ccttcgccgc acccgcgccg cgcgttcgtc atcggtggca
      481 ccggcaccat cggtcgcgcc acggtgcgcg cgctggtggg gcgggggcac gaggtggtgt
      541 gccttgttcg gccgcagtcc ggtgtcggcg gtgcgctgac gcgggatgac agcgcgaggc
      601 tgctcgccgg ggccacggtt cggctgggtg atgtcagcga tccgctatcg ctggcgcgcg
      661 acgggttttg cggagaacac ttcgatgtgc tggtctcgtg cctggcttcg cgaaccggcg
      721 cacccaagga cgcctgggcc atcgaccacg aggcacaccg gcacgcgctg acggcggctc
      781 gggaggccgg cgtgtcgcac gtcgtgctgc tgtcggccat ctgcgtgcag aagccgctgc
      841 tggccttcca gcatgccaag ctggccttcg agaaggtgct catcgagtcg ggcctgacct
      901 attcgatcgt gcggcccacg gcgtttttca aatcactgtc cgggcaggtt gatcgtgtca
      961 aggccggcaa gccgttcctg gtgttcggcg acggcacgct caccgcctgc aagccgatca
     1021 gcgacgatga cctgggccgc tacctggctg gctgtctcga cgacgagagc cgccacaacc
     1081 gggtgctgcc catcggcggg ccgggcggtg cgatcacgcc gcgccagcag ggcgagcacc
     1141 tgttcgcgct gctgggtcgg ccgccgcgct tcaagcaggt gcccgtcgcg ctgctcgacg
     1201 tgatcatcgg cgtgcttggc accctgggtc gcgtggtgcc tgcgctcgcg gccaaggccg
     1261 aactcgcgcg catcggccgc tattacgcca ccgagtcgat gctggtgttc gaccgttcca
     1321 cgggccgtta cgacgcggac gccacgccct cgacgggaac cgaaaccctg ttcgatttct
     1381 atgcgggtct ggttcgcggc gacatcgttc ccgagcgcgg agaccacgcc gtcttctgac
     1441 agggactcag ccgcgcggct cgccccggtt gttgacgctg atggtgcggc ccggtggcgt
     1501 cgtcagctga acccggtgct cctcgccgcg gaagtcgtag gtcacgtccc agtacgcagg
     1561 acggccctgc ccgggtgcgc tcgagcagcg ctgcacgttg cgggtggctg cctgctgctg
     1621 atcgccgtcg ctgcgtccga cattgccgcc aagcagcgcg ccgccgacga tgccgacccc
     1681 ggtggcaacg ttcttgccgg ttccgccacc cacctggtga ccgaggatgc cgccgatcac
     1741 cgcgccgatc agggctcccg gcacgttcgc tccgccgctc tcgcgcgtca cctgctcctg
     1801 ctctacccag cagcgctcgc ccgactcctc gaccacggcg cgcaccgaag tgatctcggc
     1861 ttcgtacagc cgttcattcc tgcggcgtcc gtaatcccgc gagaccatcg gcagcggcgc
     1921 gtagcggtca tcgtccacct gcgctccacg cgccaccact cgcgtcgacg acacacggtc
     1981 gttcaggccc atggccgaca gcgaggggta ctgccccggg cgcaacacga cacagcggcc
     2041 gcggaagttg ccgtcctcgc agacttccca gcggtcccac gggttgccgc ggacgacggc
     2101 cgacgaggcg cggtcgttga attcggtgcg ccggaagttg cgcacctgcc gctccgtcgt
     2161 gtacgtgcgg ccctggaatc gttcgttttc gtagaacacc acctggctgg tctgtgctgg
     2221 cggcgggacc ggcgcatagc ggtccgggtc cacgcgcgca ttgcgatcca cgatccttgc
     2281 cgatgacacc cggtcgttca ggcccatcgc ggacagcgac ggatactgac ccgggcgcag
     2341 ggtcacgcag cgcccccgga attgcccgtc ctcgcagacc tcccagcgat ccgagcgatt
     2401 gccgctgacg accgccgaag aggcccggtc gttgaagccg aactccgaga agttgcgcac
     2461 cggctgcgcg gtcgtgaagg ttcggccgcg aaagtcgctg gattcgtaga aggtcacctg
     2521 cgcggtggcc tgagcagcga tcgcgaggcc ggtgagggcg atcgcgatgt ggaaggtctt
     2581 gttcatgatg ttcacccggg ccgcgcgacg aacacctgcg gccgtgggcg gccgtgaggc
     2641 tgaagcgagt gaacgagtct gggatcgcga gccctgcgcg cccatcagac ggaggcttct
     2701 cgatgcgtgg cgcggcgtcc tacgacgacg gtgcgcggcg tgcagtcaag ccgccgaaat
     2761 cagtcggcga ccttcgacga gaacctgaat ccgccgtgac cacgggcctt caccgtgtac
     2821 atcgcgttgt cggcggtggc cagaagctcg ctcaccgtct ggccgtcctg cggatagcgg
     2881 ctgatgccga tgctggcgcc gaccttgagc tcgaccccgt tgatgtcgat cggctcgcgg
     2941 atgccggcga gcagcttctc ggccactgcg accagctggt cgtgcgaagt ggatgcgccc
     3001 agcaacgcga cgaattcgtc gccgccgatg cgtgcgatgg tgtcgccctc ccgcatcatc
     3061 gtcagcagcc gctgcgcgat ggccttcagg acgatgtcgc cggtgtcgtg accgcagcga
     3121 tcgttgacgt tcttgaagtc gtccaggtcg atgaacagca cgctggcctc gcctccgtag
     3181 cggcgcgcgt gggccatggc cttgtcgagc cgggacacga acaggcgccg gttgggcagt
     3241 ccggtcagca tgtcgtgctg cgccatgtgc tccagttcct gctggttgtt ctgcaggtcc
     3301 tcgagctgct tcgtgatctg gtcctgcatc ttctggaagc tgcgtgcgag cgcgccgatc
     3361 tcgtcctgcc gttccaccgg caacgccgcg gacggatgcc gggatgcgaa accctgcgcc
     3421 gccgattcga ggatgttgat cggccgcgtg aatgcgcggg ccagcagcgc ggcgagcagc
     3481 aggcagccca ggcacaggcc gagcacgatc tgcaggatcg cgatgcccag ctgatgcgcc
     3541 tgcgcgacga cttcggcccg cggctgcgcc agcccgagga tcagccgctg ttcgtgagaa
     3601 gggatctcga cgagctgact gatgaaggcg gcgaccaccg gcacgtcgct gcccgaaggc
     3661 tcgcctgcgt cgaacacgac atgcgcgacg cgcccttcga cgacgtctcg tgtcgggggg
     3721 aactcctcct cgatcagcac ccgtcggccc ttgtcgaacc cgaaggtgcg cctggcatcc
     3781 gggtggatca ggatgtcgcc cttgtcgttg gccagaaaca gcctgaagct cgcaggcaga
     3841 tccgccgcca gcagcgagaa catgccgttc aggtcgatgt tgaccacgat cacgccgatc
     3901 gccgtgccgc ccgcatcggt gaccggcgtg gccagctgca gtgccggctc gtccaggccg
     3961 gcgtacgtgc cccgttcatg gttgaccgcg atctccgaca ggtagaactc gccggcctgc
     4021 agcttcaggg tttcggcgac gtacgggtag tgccccttct cctggagttc gtcgtccttg
     4081 acctccagca gcgcatcgcc ctgccggtcg acccgaaccc gctccaggcc atggtcgctg
     4141 gccgagatca ggcgaatctg gaagtacgac gggttctccg ccatcagcag gcagaacagc
     4201 gtggacaact ggcccgcctg cgcggcgtcg cccgactgca gcaccgactt gctggcggga
     4261 tggcgggcca gcaccccgag gttgcgcgtg atctcctgcc gcgacagcgc gatcctccgc
     4321 gccagcacct gcgtcgacgt cagcagcttg tcctgtgccg attcgatgag cagttgccgg
     4381 ctgctctggt acgcgtacag gccagtcagc cccgatgcga gcgctccgac gagtgtcagc
     4441 agcagcgcga gcttgcgggc gatgcccagc ttcacgaggc aagcaccttg ctggcgcgat
     4501 ctccggacag gatgcggccc cacagctggt tccggtgttc ctcgctttcc accggctgca
     4561 gccagcgcag cttctcgccg gcttctccgg cggactcggc cgacgtggtg ctgcccagtc
     4621 cctgacgcgt caccagcgcc tggctgggcg cgtcttcgag caggtggttg atccaggctg
     4681 ccgcgagctt cgggttgcgc gcaccgcgcg tgaccaccca gcagtccagc cacgccagcg
     4741 ccccttcgcg gggaagcgca tagcccacgt cgaggccggc ggccttgcac atctgcagtt
     4801 gctgcttgcc gtagttggcg aacatcagcg ccgccttgcg gcgcttgaac aggcccaccg
     4861 attcatcggg ccgtgtgtag aaacccgtca cgttgcggcg cagggcgatc aggcgatcca
     4921 cggcacgcgg ccagtccttg tcgtccaggc ggaacggtga cttcaggccc agcgacagcg
     4981 ccgccagcga aaaattgtgc gtgccgccgt tgtaggcgat cacctcgccg tgatagcgct
     5041 cgtcccagag cgcctcgatc gagtcgggcg cgctcgccat ccgttgccgg tcgtagatca
     5101 ggcccatctc cgaataggtg tacgggatgg cgtagaccgt gccgttgcgc acgatgcccg
     5161 gaatcgccgc cagatcccga aagcgcgggc gctggcgggc caggttgggg atcgagtcgg
     5221 ccgcgatgcc gaccaccagg cccttgcgga tgtagcgctg cagttcggcc gtgttcaccg
     5281 cgaagacatc gaagtcctgg gcgctgttcg cggtgatctt cgtccacagg tcgagatccg
     5341 aatcgatgag cgtgacctcg acccggctgc cgtgcttctg ctcgaacgac cggacgaagt
     5401 ccgcatcggc atacccgggc cacgccagca cgcgcaacgt ctctgccgcg cgcgcccagc
     5461 ccggaactgc ggcagcgagg gcgccgcaga attgacggcg attggggtgc gggatcggca
     5521 tcggtgacaa cggttgggac tggggtctgg gacctgggac ctgggaagtt acgacatccg
     5581 tcgaacgcag gcgagcgatt caagccccgg tatcagggtt atcggcaggt cagccccgtt
     5641 cttgcggtgc tctaaccagg atggactgga acatggggcc ctgacatggc cttgactcgc
     5701 gatataggac gtttcgagcc gattctccgg aataagatcg gagccatctg tccagatcgc
     5761 ggccgtccgg acaccgacga agcttcattt gcgccacacc ataccgacaa ccggatgatc
     5821 ggtttgttca aattcagggg ttcacgtgac gcaaagaacg atattgatcg ttgacgacaa
     5881 cccggaaaac ctgacggtac tgggtgaatt gctctgcgag cgctacacgg tgcgcgcggc
     5941 caattcgggc gcccgggcgc tgcaactggc gatgcagact ccgctgcccg atctgatcct
     6001 gctcgacatc atgatgcccg gcatgagcgg ctacgaggtg ctcgaacggt tgcaggacac
     6061 cccgaagctg cgcgacatcc cggtcatctt caccaccgcg atgaatgccg aggatgacga
     6121 gcagcgcggc atcgccctgg gcgcggtcga ctacatcacc aagccgctga agcccaccgt
     6181 cgtgctcgcc cgggtgcagg cgcatctgga actgaagctg gcgcgcgacc ggctgcagca
     6241 gatcaatgaa tcgctggaag ccgagattgg ccggcgcatg cgcgagaacc tgaccatcca
     6301 ggacgtcacg atccgcgcgc tggcaaggct ggccgaaact cgcgacaacg agaccggcaa
     6361 ccacatcctg cgcacgcagc gctatgtgca cgcgctgact gagcgggcca gctgccatcc
     6421 gcgctttgcc ggcgaactcg acgagcagag cgtcgcactg atcgccaagt cggccccgct
     6481 gcacgacatc ggcaaggtcg gcattcccga ctgcgtgctg ctcaagcccg gcaagctgac
     6541 tgccgaggag tgggacatca tgcagacgca tgccgccatc ggcgccgacg cgatcagccg
     6601 cgcggaggcc gacacctgcg agccggtgaa gtttctcgaa ttcgccaagc aggtcgcccg
     6661 ccaccaccac gagcgctggg acggtggcgg ctacccggac ggtctggccg gcgatgcgat
     6721 tccgctggcc gcgcggctga tggcggtggc cgatgtcttc gacgcgctga tctcccggcg
     6781 tgtctacaag aaggcgttca cgctggacga ggcgcggcac atcatggcct cgcagggcgg
     6841 caagcatttc gaccccgacc tgctgcagtt gtttctcgat ggattcgacg agttctgcga
     6901 gatcgcccgc aggcatcccg acagccccga gccggctgaa tcggtggccg agaccgcgcc
     6961 cgctgccatc gcggtggcag cgacttgaac tgaactgaac tgcgccggcc ggaccggcat
     7021 tcttgatggc gccattcgac aagcgcgaat ttcaggtgga tctgctgcgg cacggcgcgc
     7081 tgcgcatcgc gctgctctat gccctgttcg gggcactctg gatcctcggt tccgactggc
     7141 tgctgcacaa gctggtgagc gacccgtcct gggtcgtgca gatcggcgcg atcaagggat
     7201 ggttcttcgt cggcgtgacg gcgtggatgc tgtatctgct gatacggcgg attccttccg
     7261 gcgcccaccc atcgaagcgg gtctctaagg ccgtcccggc gcagcgggct ggctgggcgc
     7321 tgctctacgt gtcgatcgtc gcgctgacgc tggtggcgct gcgtttcgat taccagcacc
     7381 agcacgagct ggagcgaacc accctccata ccctggctga gctgcaggcc cggctggttg
     7441 cgcacggcta cgaggatcgg gccgtgcagg cccgcttcat cagcagcagc ccgcacc
//
LOCUS       DAOORV010000160         7486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12163_length_7486_cov_7.529404, whole genome shotgun sequence.
ACCESSION   DAOORV010000160 DAOORV010000000
VERSION     DAOORV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12163_length_7486_cov_7.529404"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1107
                     /locus_tag="PKV56_15585"
     CDS             289..1107
                     /locus_tag="PKV56_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69282.1"
                     /translation="MENLFRFCDPHASFSKGSKGRRSRKVFTKWGESNLGIDAWKVGL
                     KAYEQFSSACGKEATLADLKCFVTASLPTLEEFGSDFEELVRAKRIELIDYDSGKAIK
                     SAPDWKSFSWNWQMRMMLQCIVAGLNAMGHGEKLEAIRSQFAVTALAELDDAVVASLD
                     NDTAAVLSSVMNATWLMDQADAAGRVKEVATVMLGKLDSQRAAIRARVRHAKDPRRLA
                     RDFVYQCWLDWKAKPSQYPSAAAFARSMLDKEPDKLRSEVVITRWVRGWAKESK"
     gene            1223..1444
                     /locus_tag="PKV56_15590"
     CDS             1223..1444
                     /locus_tag="PKV56_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOX69283.1"
                     /translation="MAANTIQQAQSDNALARRRQVEHLVQLSRSSIYAAVKAGTFPAP
                     YRIGARAVAWRVSEIEQWLEARPLASTTA"
     gene            1459..1776
                     /locus_tag="PKV56_15595"
     CDS             1459..1776
                     /locus_tag="PKV56_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX69284.1"
                     /translation="MNEKKATPINEAARNNLHTKSSACDNSNDAAAQRARALDALRIE
                     PRDTIQLRRDYDILAPAARIWELKHRFGFEITKLMVRRPTACGKLHRVALYVLMRETG
                     GHA"
     gene            1773..1919
                     /locus_tag="PKV56_15600"
     CDS             1773..1919
                     /locus_tag="PKV56_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69285.1"
                     /translation="MTAPTWLERLQWLAARFPEHGIGTDLAALSAADLWGLYRFLTRI
                     ANGG"
     gene            1922..2518
                     /locus_tag="PKV56_15605"
     CDS             1922..2518
                     /locus_tag="PKV56_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69286.1"
                     /translation="MSTKKFKGNRDKRDGRQFLLLPHVVLESPGYRLATHPARSLLID
                     IAMQYTGHNNGKLVACAKYLKGKGWNSNATIVRARHNLIDCGLLIETRKGARPNKAAW
                     FALSWLALDQGQGLDIDPKFYRRGAYMTPDKAAQQNATFAPAGGAVVMSIAPARGARA
                     SNVAPAGGAIRPPVHTTPAPSGGAYLETPSVPVRKHHC"
     gene            complement(2521..3870)
                     /locus_tag="PKV56_15610"
     CDS             complement(2521..3870)
                     /locus_tag="PKV56_15610"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HOX69287.1"
                     /translation="MAFTDFLHGPKYRRQSEQLLDQVGRLTAEIDRLTDQCERLRGLS
                     KKLGVLDLVQVQDIIANEKSLLQQVEASHALKASELEGMTAQIKALADQRLVIEEHLL
                     LESFALYIPKFAFTASADYKAKLDSVRARQKEAIKAGTAASGNQGWSVNNSQAEGRKL
                     VNDMIKLVIRSFNNEADFCVDNVKFDNIDLGEKRINKSFETLNKLGRIMTVRLSDEYL
                     EMKLDELRLAHEFQVKKQQEKEEAKKAREELREQQKLEQEIRAAREKIAKERKHFLAA
                     LKDLKIRLAASADDIERGKLESKIAEIAGSCSALDGEEQLIDYREKNAKAGYVYVISN
                     LGAFGEDVYKIGMTRRLEPLERVDELGDASVPFYFDIHALVFSDNAPGLEAKLHERFH
                     KSRLNKINPRKEFFRAKIEEIEAVIRENFDSVVEVVREAPAEHFRESLRLVSMESAR"
     gene            4175..4597
                     /locus_tag="PKV56_15615"
     CDS             4175..4597
                     /locus_tag="PKV56_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69288.1"
                     /translation="MTLNYSIDDQPRTQTISVNETACNFGGTRQWFICPIRFERVAVL
                     YLRAGRFACRHCQRLAYASQSNDAIGRTWRRQHKIERKLGENWQRPKGMHQTTYDRLL
                     STIGDCEEKRDAALSRFLAGMMDRHPSLRSDPMLRGMF"
     gene            complement(5062..5901)
                     /locus_tag="PKV56_15620"
     CDS             complement(5062..5901)
                     /locus_tag="PKV56_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69289.1"
                     /translation="MKSTTKLVALAALICAPSAWAAPVFTTFEAVSLGAAGLTPTVDA
                     FRAAISGPRREINWDGVPDTRADPNALPADFFNATSPRGVVFSTPGTGFLVSANAGLP
                     TPTLFGFGSDFAAFSAQRLFTAVDSNITDVRFYEPGTLNPATTSAFGVVFTDVEVADL
                     TQLEFFDAADVLIYSRYVQPGGNQGFSFLGATVNDGSVGRVRITAGLNTISANGQLGN
                     PNDDVVVMDDFIYAEPTRTHDVPEPSGLALAGLTLMGALIGSLRRRGKSVRQSDDTFN
                     MRP"
     gene            complement(6265..6591)
                     /locus_tag="PKV56_15625"
     CDS             complement(6265..6591)
                     /locus_tag="PKV56_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69290.1"
                     /translation="MLNPTQVIEIRRDPSVDGARSLTRAARQWVRSLPARLRPLSLCS
                     SYARLANRMAAVWEDPEQTEALFDELLIDHRGGRHGFPPLVAGELMRLHHFHERRLAP
                     ASRLDG"
     gene            6849..>7486
                     /locus_tag="PKV56_15630"
     CDS             6849..>7486
                     /locus_tag="PKV56_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOX69291.1"
                     /translation="MGTKLKISGWIALGAVAGALATMQLQATARNAVSSLPLEELQQM
                     AAVFGMVKSDYVEPVDEKKLITDAISGMVAGLDPHSQYFDKKSWKEFREGTTGRFVGV
                     GIEIGMEDGLVKVVSPIEGSPAFRAGLKSGDLITKIDDVAVKGLTLDQAVKRMRGEPG
                     TKVVLTMFRKAESRSFPVTIVREEIRTVSVRAKLIEPGYAWLRVSQFQDRTV"
BASE COUNT         1569 a         2208 c         2275 g         1434 t
ORIGIN      
        1 accacatcga gaaacaactc gcccacgaag atcgcgacga cgttcgcggc gcctacaacc
       61 gcgcgcagtg gctcgacgag cgcacgaaga tgatgcagtg gtgggcagat cgcatcgatc
      121 agttgcgcaa gggcgcagac gtgattccgt tcaaggcggc ttgatcgaga tgtagatcac
      181 catcgatttc gccacgccta gcccgaggct gatccccgag ggcgaacccc gggttccctt
      241 gcccggctgg cgtggctccc tcacaaggaa tagtgccaag gggcgccaat ggagaacctg
      301 tttcgctttt gcgatcctca cgcgtcattc agcaaggggt cgaagggtcg gcgctcgcgc
      361 aaagtcttta ccaagtgggg tgagtccaat cttggaatag atgcttggaa ggtcggccta
      421 aaagcatatg agcagttcag cagcgcatgc ggaaaagagg caacactggc tgacctgaaa
      481 tgcttcgtga cggcctcact cccgactctg gaagagttcg gctcggactt tgaagaattg
      541 gtccgagcaa agcgaatcga gctgattgat tacgattccg gcaaggccat caagtcagca
      601 ccggactgga aatctttcag ttggaattgg cagatgagaa tgatgctgca gtgcattgtt
      661 gcgggcctta acgccatggg acacggcgag aaactggaag caattcgttc ccaattcgcc
      721 gtcaccgcgt tagctgaact tgatgacgca gttgttgcga gcctcgacaa cgacacggcg
      781 gctgtcctga gttcggtgat gaacgctact tggctgatgg atcaggctga tgcggcaggt
      841 cgggttaagg aggttgcgac agtaatgctc gggaaattgg atagccagcg agcagcgatc
      901 cgagcaagag ttcgccacgc caaagacccg cgccgcttag cccgagactt cgtctaccaa
      961 tgttggctcg actggaaagc aaagccaagc caatacccaa gtgcagcagc cttcgctcga
     1021 tcgatgctgg acaaggagcc agacaaattg agaagcgagg tcgtgatcac tcgctgggtg
     1081 cgtggctggg cgaaggagag caaatagcag cctgcccgca aagtgcgcat cgtgccggct
     1141 gaatgctttt cgacagcgcg aatttgccaa tgatgagaag ccatgaagtc cggggggttc
     1201 ccggacgttg cagaaagcaa ccatggcagc gaacacgatc cagcaggcgc aatccgacaa
     1261 cgcccttgca agacggcgac aggttgaaca cctggttcaa ctctcacgat ccagcatcta
     1321 cgcggcagtg aaggcgggca cgttcccggc gccatatcgc atcggcgcgc gggctgtcgc
     1381 atggcgagtc tccgagatcg agcaatggct cgaagcccgg ccgctggcaa gcaccacggc
     1441 ctgatcggag ggaacgccat gaacgaaaaa aaggccaccc cgatcaacga agcggcccgg
     1501 aacaatttgc acacgaagtc tagtgcctgc gacaacagca acgacgctgc agcgcagcgc
     1561 gcgcgggctc tcgatgcact tcgcatcgaa cccagagaca cgatccaact ccgccgcgac
     1621 tacgacatcc tcgcgcccgc tgcgcgcatc tgggaactga aacatcgctt cggtttcgag
     1681 atcacgaaat tgatggtgcg acgacctacc gcgtgcggca agctgcaccg ggtggcgctg
     1741 tatgtgctga tgcgcgagac aggcgggcac gcatgacggc cccgacctgg ctggaacggc
     1801 tgcaatggct cgcagcacgc tttcccgagc acggcatcgg caccgacctg gcggcgctgt
     1861 ctgcggccga tctgtggggc ctgtatcggt tcctgacgcg catcgcaaat gggggctgac
     1921 catgagtacc aagaagttca agggcaaccg ggacaagcgc gacggcaggc agttcctgct
     1981 actccctcat gtcgtgctcg aaagccccgg gtatcggctc gccacgcacc ctgctcgatc
     2041 gctgctgatc gacatcgcca tgcagtacac cggccacaac aacgggaagc tggtggcctg
     2101 cgcgaagtac ctcaagggca agggatggaa cagcaacgcg acgatcgtgc gggcgcggca
     2161 caacttgatc gactgcggcc tgttgatcga aacccgcaag ggtgcgcgcc cgaacaaggc
     2221 agcgtggttc gcgctgtcgt ggctggccct cgatcaaggg caagggctgg acatcgatcc
     2281 gaagttctac agacgcggcg cgtacatgac tccggacaag gcagcgcagc aaaacgcaac
     2341 gtttgcaccc gctggcggtg cagtggtgat gtcgattgca ccggctcgcg gtgcaagggc
     2401 atcaaacgtt gcacccgctg gaggtgctat ccgaccccct gtgcacacca cgcctgcacc
     2461 ctctggcggt gcgtatctag aaacaccatc tgtcccagtg cgtaagcatc attgctaggc
     2521 tcatcgagcc gattccatgg aaacgagacg caggctttcc cgaaagtgct ccgctggtgc
     2581 ttcgcgaaca acttcgacca cagaatcgaa gttctcccgg atgacagcct cgatctcctc
     2641 aatcttcgcg cggaagaact ctttgcgagg attgatcttg ttcaagcggg atttgtgaaa
     2701 ccgctcatgg agcttggcct caagcccggg cgcgttatca gagaacacca aggcatgaat
     2761 gtcgaagtag aaaggaacag aagcatctcc cagttcatcc accctttcaa gcggctcaag
     2821 gcgcctggtc atgccgatct tgtaaacatc ttcgccaaat gccccgagat ttgaaatgac
     2881 gtacacatag ccagccttcg cattcttttc gcggtagtca atcagttgct cctcgccatc
     2941 aagtgcgctg caactgccag cgatttctgc aatcttgctt tcgagtttcc ccctctcaat
     3001 gtcatccgcc gacgctgcca gccggatctt tagatcctta agcgcagcca gaaaatgttt
     3061 gcgttccttc gcaatctttt cgcgagcagc tcgaatttcc tgttcgagct tctgctgttc
     3121 acgaagctcc tctcgtgctt tcttggcttc ttccttttcc tgctgcttct tgacttgaaa
     3181 ctcgtgagca agccgaagtt cgtccaactt catctccaga tactcgtcgg ataggcgcac
     3241 agtcatgatg cgtccgagct tattgagtgt ctcgaatgac ttgttgatcc gcttttcacc
     3301 gagatctatg ttgtcgaact tgacgttgtc tacacagaag tcagcctcgt tattgaatga
     3361 acggatgacc agtttgatca tgtcgttaac gagttttcga ccctcggctt ggctgttgtt
     3421 gacgctccat ccttgattgc ccgaagcggc tgttccagcc ttgatcgctt ccttctgccg
     3481 cgcccgaaca ctatcgagct tggccttgta atcggcactg gcggtgaagg cgaactttgg
     3541 gatgtacaaa gcaaagctct caagcagtag atgctcttca ataacgagtc gttgatctgc
     3601 aagcgccttg atctgggcgg tcatgccttc aagttcagac gccttcaacg cgtggctggc
     3661 ctcaacctgc tgtagcagcg acttctcgtt cgcgatgatg tcctgaactt gcacgagatc
     3721 gagaacccct agctttttgg acaagccccg cagtcgctca cattgatcgg tcagccgatc
     3781 gatctctgca gtcaatcgtc caacttgatc caggagttgt tcgctttgcc gacggtactt
     3841 cggaccatgt aagaagtcag tgaaagccat tgctccccct tgctcggcgc aggtgccgat
     3901 ccaagtctac gaggagcatg ctcagcctcg ctattcttag attcgctatt gatttcgttt
     3961 tctgtggagc gctgaaatgg gtacaggtgg cagcagatcg ggcgcaggca gaccggccat
     4021 acatgtcaag gccgagtact gtctgcgcct cgatgttcga cagttgtgtc gcgcgggagc
     4081 aatgcaggcg ggtcgatcgg gttcatggca ttggacgaac cagacaaccg gcagggagag
     4141 cggctcgatt ggctactcgg tccggccggg ctgcgtgacg ctgaactact cgatcgacga
     4201 tcaaccccgc acgcagacga tctcggtcaa cgagacggcc tgcaacttcg gcggcacgcg
     4261 gcagtggttc atctgcccga tccgtttcga gcgcgtggcc gtgctgtacc tgcgtgcagg
     4321 tcgcttcgcc tgccggcact gccagcgact ggcgtatgcg agtcagtcga atgatgcaat
     4381 cggccgcacc tggcgccgac aacacaagat cgagcggaaa ctcggcgaga actggcagcg
     4441 gccgaagggc atgcaccaga ccacgtatga ccgactgctc tcaacgatcg gggactgcga
     4501 agaaaagaga gatgccgcgc tcagccgttt tctggcgggg atgatggacc ggcacccctc
     4561 gttgcgcagc gacccgatgt tgcgcggaat gttctagccg agatcgcgtg ggggtagatt
     4621 tgggggtaga tcacccgaat ttcattcccg tagcccggta gttcgattcc tgtcggcggg
     4681 accaatgaca agtgatgcgg cgcctcgcgc tgcgatttga aaacgcctac ctcggtcgtg
     4741 gaacgggcaa cccgttacca gcgggtggcg tgtcaacctc gaaactggcc cacaacgaat
     4801 cggagggatc gatctcggcc acctccggct tgcgcggctc gacgccgcgg cgccgatccc
     4861 ggcccgccgg catctcgaca tcgcgcacgc gccggtctcg tccagtacgt cgatctgacg
     4921 caggctggcc cggcttggat gtgttgatgt tcatcgacgc gattcttgct gaatctgccg
     4981 ttgcaggcgc cgtgcagttc gggattagta ggcaaacgcc gactttcggg ccgatacgcg
     5041 ctcgcgcagt caggttcccg atcagggacg catattgaag gtgtcgtcgg actgacgtac
     5101 tgatttaccg cgacggcgaa gagacccgat cagtgcgccc atcaaagtca gacccgcaag
     5161 cgccagaccg gagggctccg gaacgtcatg ggttcgagtc ggttcggcgt agatgaaatc
     5221 gtccatcacc acgacgtcgt cgttcgggtt gccgagttgc ccgttggcgc tgatcgtgtt
     5281 caaacctgca gtgatgcgca cgcggccgac ggatccgtcg ttcaccgtgg cacccaggaa
     5341 gctgaaacct tgattgccgc cgggctgcac gtaccggctg tagatcaaga catcggcagc
     5401 gtcgaagaat tccaattggg tcagatctgc cacttcgaca tcggtgaaga ccacgccgaa
     5461 agcactcgtc gtcgcgggat tcaaggtgcc cggctcgtag aaacggacat cggtgatgtt
     5521 gctgtcgacc gcggtgaaca gtctctgggc actgaaggcc gcgaaatcgg agccgaagcc
     5581 gaaaagcgtc ggtgtcggca agcccgcgtt cgcgctcacg aggaagccgg ttccgggtgt
     5641 gctgaacacc acaccgcgcg gcgaggtggc attgaagaag tccgctggca gcgcgttggg
     5701 atcggctcgg gtatcgggca ctccgtccca gttgatctcc cggcgcggcc cgctgatcgc
     5761 tgcacgaaag gcgtcgacag tcggcgtgag gccggccgca ccaagactca ccgcctcgaa
     5821 agtcgtgaac accggagcgg cccaggccga cggtgcgcag atcaacgcgg caagggcgac
     5881 cagcttcgtg gttgacttca tggcaggtgt tccttgttcg aagtaatgac accacggctg
     5941 tcgccgcgca ggagcaagga aacaggatga gccaggagcc ggcatcgaac gtgatggcgg
     6001 aaacatggcc agaccgatca caccacccgg atgccgttgc atccggagca taggtcgcat
     6061 tgcgtgttcg gcgcgatatc gtcgaacccc cggttcgagg gggccgcaag acagaggctg
     6121 cgctgagtgc ccggcagatc ggccgcgtgg aggcctcatg agcagggccg cacctcgagc
     6181 tagggcgaca ggggtcgcca gcgggaatcg acccgaactg cgctctatcg cctattcaaa
     6241 aggctgtggc gcgtccgaag gcattcaacc atccagccgt gacgccgggg ccaggcgccg
     6301 ttcatggaaa tgatgcaagc gcatcagctc gccggcaacc agcggaggga aaccgtggcg
     6361 accgccgcgg tggtcgatca gcaactcgtc gaacagcgct tcggtctgct ccgggtcttc
     6421 ccagaccgcg gccatccggt tggccagtcg cgcgtaactt gagcacagcg acagcgggcg
     6481 caaccgggct ggcagcgagc gcacccactg ccgcgccgcc cgggtcagcg accgggcccc
     6541 gtcgacactc gggtcgcgac ggatctcaat gacctgggtt ggattcagca tgtggacggc
     6601 cttttctccg ggcaacgtcg gtccggagcc cggccgatct cagtcgctat cggccgggaa
     6661 cgccatcact tgacactttc ctcgcccccg cgaatccacg atccgcgaca gactgggtgc
     6721 cggcgccgga aatctttggc ttagcccggg caaacgctga aacttaccgg cgccggcaag
     6781 catccataca accctcgggt tctatactga agcgtcacag catgcacaag tcagcaagag
     6841 ggtcggacat gggtaccaag ctcaagattt cgggatggat tgccctcgga gcggtggctg
     6901 gtgcattggc cacgatgcag ttgcaggcga ccgcgcgcaa tgccgtcagt tcgcttccgc
     6961 tcgaggaatt gcagcagatg gctgcggtct tcggcatggt caagagcgac tacgtcgagc
     7021 cggtcgacga gaagaagctg atcaccgacg cgatcagcgg catggtggcc gggctcgatc
     7081 cgcattcgca gtatttcgac aagaaatcgt ggaaggaatt ccgcgaaggc acgaccgggc
     7141 gtttcgtggg tgtcggcata gaaatcggca tggaagacgg gctggtcaag gtggtttcgc
     7201 cgatcgaggg ttcgcctgcg tttcgtgccg ggctcaagag cggcgacctg atcaccaaga
     7261 tcgacgacgt tgcggtcaag ggactcacgc tcgaccaggc cgtcaagcgc atgcgcggag
     7321 agccgggtac caaggtcgtg ctgacgatgt tccgcaaggc cgaaagccgc agctttccgg
     7381 tcaccatcgt gcgcgaggaa atccgcacgg tcagcgtgcg cgccaagctg atcgagccgg
     7441 gctatgcgtg gctgcgggtc agccagttcc aggaccgcac ggtcga
//
LOCUS       DAOORV010000161         7408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12323_length_7408_cov_11.046376, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000161 DAOORV010000000
VERSION     DAOORV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_7408_cov_11.046376"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..667
                     /locus_tag="PKV56_15635"
     CDS             191..667
                     /locus_tag="PKV56_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69292.1"
                     /translation="MYIRTPRIGAVCSADPNDIQALAGDFAYELRQFFKTERDGERRR
                     ATSAAIDAFDKAGDDPEALQAFVDGVGLEAIQAYCLPFMSFSLAPSGDYGFWPDLDGL
                     ECAARSKEGVVKVSAGDPWPPMWSGSGRDIQLVMEVNDHGNVTLYNRRRNEIWSCV"
     gene            complement(965..1843)
                     /locus_tag="PKV56_15640"
     CDS             complement(965..1843)
                     /locus_tag="PKV56_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOX69293.1"
                     /translation="MIDELAIRLKARQFMAGLDLSNIDKDLTVYTRKVNAKLTTEEMS
                     PGESGYTLTRRDGKSSIVVNELERRERQRFSACHEVGHLVLALASNHQETPAWSYAKR
                     DDNEIACDIFASELLMPFDAFKRDVDQDQPSFELIERLRSKYVVSFAACASRLAAVTD
                     YPCAFVFMNATVVRYASRSKALRDLNAWVEMRSPIPPGSVAHRLVQEGEWSGEDSRVA
                     QDVWFRDWPNGYDLTEMAKHYPTFEETFSLLWFEPDGGPDEPVKVWSGSVAQEDDGGL
                     QELDGVLSFQKKTKKR"
     gene            complement(1840..2217)
                     /locus_tag="PKV56_15645"
     CDS             complement(1840..2217)
                     /locus_tag="PKV56_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX69294.1"
                     /translation="MAQSGLGMLLRKLREARDLSSRELGQLADIDHTYIYRLETGEKQ
                     SPSSELMERLLRVLKAKPREVEMAQFMMSNEVNPDWVEHVLGTDLTAEMFEMGATMRH
                     RGAGRPDMEAIAARVRKALEDDE"
     gene            2393..2575
                     /locus_tag="PKV56_15650"
     CDS             2393..2575
                     /locus_tag="PKV56_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69295.1"
                     /translation="MAKNKPIGDNARVGAVRDRSQVFNPLTQTWTKRDASSGRFMDQK
                     ADGDPFKGVRKEKPVK"
     gene            2630..3886
                     /locus_tag="PKV56_15655"
     CDS             2630..3886
                     /locus_tag="PKV56_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004536106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOX69296.1"
                     /translation="MHRDFSIEPPRRRFSVGASARFFYLGEAIARVYEPTQTQLSDLD
                     RAYQSTGEYLVTCPEFDGLLYLVHAHGSRQLGTMVRPMEEYREGFDIDLAACLTREGL
                     QRYGGAGGPARLINDLYAALKRYADRHNLKIERDDRCVTITYAGGMKADFAPIIDDAS
                     HVVLHGETHGRIPDRDLQRYMSTNPRGYCRAFDSIAQVAPAFELRVELNATFESLRKS
                     ELLPLPDAEEVFARLLSRLTQLAKINRNIAFGAPTSGENLAPPSCVVTTLVANAYAIE
                     APKPHDGPMDLLLDIVQLMPRLIKRFPMPEGSEFWYLDNPTAQNDNLAAGMNTKAKQQ
                     AFDAWCERLVADLTSLVDAIDQGAGMDVIARAVERAFGPRARAAVLGDNAQRREANRS
                     ANRGIFVVGSSAVVSTPTFAHTNFGD"
     gene            3935..4366
                     /locus_tag="PKV56_15660"
     CDS             3935..4366
                     /locus_tag="PKV56_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69297.1"
                     /translation="MELRALNFPGARVRLLHGRELRYSFSISPTVFSRLYRCVLVLTP
                     AQSPQMFVVNPDLRALAKGQPLPHIYHYDGPGVRLCLWLPRKNEWLPQMRLLETYVAW
                     TSEWLNYFEEWLVTGEWAGGGEHPPPRKKRRLLRATRPVTT"
     gene            4363..5262
                     /locus_tag="PKV56_15665"
     CDS             4363..5262
                     /locus_tag="PKV56_15665"
                     /inference="COORDINATES: protein motif:HMM:NF037206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="HOX69298.1"
                     /translation="MNNIPQRQLLPAMLSLVRARTLAYRRAKRCQAFVLALNLLLPVV
                     SAAAAVFMPDARPAIAVCALLLGIFDVTAIDAWMKARLKVGAKLQEEFDCEVLDIDWN
                     EFVAGTKMDAEASYEDGCRTLDDVDERRLRDWYPVVVAETSLEVARVICQRENLVYDS
                     DLRVAYRRVLFWFVVVFVVVFSAASLAFNPSFMSLVLALVPAAPALTWALRENKRQGD
                     TLETLTRLKAESEKLLKATINGVDAAEAKRRSRELQDAIFGHRLSSSLVFDWLYNLLR
                     PRLEAKLVHGAKRWVDEAKRSGT"
     gene            complement(5377..6495)
                     /locus_tag="PKV56_15670"
     CDS             complement(5377..6495)
                     /locus_tag="PKV56_15670"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOX69299.1"
                     /translation="MSIRKTAKKTAVAHRDSRSGAAADVPVESAFGEVVDLIRAARQR
                     AHQAVNTELIGLYWQIGEYINGKLAAAEWGEGVVERLAQHLARTEPGLRGFSAQNLWR
                     MRQLFETYRDAPKLSPLVRALPWTHNLIILGQSKRAEEREFYLRMAVQQRWGKRELER
                     QIRLGAFEQAVLAPAKLSPAVREIHGESAATVFKDAYALEFLELPVGHTEADLHRGLL
                     GRLQTFLIELGRDFCFVGSEFPLQVGGRDFALDLLFFHRGLNCLVAIELKVDRFEPEH
                     LGKLNFYLEALDRDVRKPHESPAIGVLLCASKDSEVVEYALSRALSPALVAQYQTQLP
                     DKQMLAAKLHEFYNLNEPAASTRESRNQTDATKVGKKR"
     gene            complement(6596..7177)
                     /locus_tag="PKV56_15675"
     CDS             complement(6596..7177)
                     /locus_tag="PKV56_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69300.1"
                     /translation="MGTNASPAVLLRRIAHLEAAVGRLTEDLESLRMAVSRHGFASSL
                     AGGLRAQVARSPIPTGLAGALARGEATKVQWVKEGLVVPGEQLARAWGLTRQALRPAA
                     GRGEVFAVKIGNRLFYPRAFLALDRQAVATVCRALGDLDASEKMIFWLREHGAVGGKG
                     IAAALDGGTAVVKAQRLAAAWARERGGGRVVAA"
BASE COUNT         1318 a         2452 c         2248 g         1390 t
ORIGIN      
        1 cgccgttgtt gcgggtggcc gcgctgcggt ggctcatcca ttgggctccg ttggacatca
       61 ccaagggcgc cccttatctg cagcggcggc gctacgtgcg ccagcactac cacgtgtagc
      121 cgccaccacc ctagatccga agaccacggc ctccaagccg tggccttccg ctccaccaag
      181 gagtcagacc gtgtacatcc gaaccccgcg gatcggtgcc gtttgctcgg ccgatccaaa
      241 cgacatccaa gccctcgctg gtgacttcgc ctacgagctg cgccagttct tcaagaccga
      301 gcgcgatggc gagcgtcgtc gcgccacgtc ggcggcgatc gacgccttcg acaaggctgg
      361 cgacgatccg gaggcccttc aagccttcgt tgacggtgtg gggcttgagg ccattcaggc
      421 gtattgccta ccgttcatga gcttctcgct cgcgccctcc ggtgactatg gcttctggcc
      481 agacctcgac ggactcgagt gcgcggcccg atcaaaggaa ggcgtcgtca aggtcagcgc
      541 tggcgacccc tggccgccga tgtggtccgg gtcaggccgt gacatccaac tcgtcatgga
      601 ggtcaacgac cacggcaacg tgacgctcta caaccgccgg cgaaacgaga tctggagttg
      661 cgtttagctg cgctctctac atctgtgttc agacgccaac gcgagcgaag gaaaaggcct
      721 tcgatcgagc cctttggcct gcaagggatg tttggcgggg ccggtcttcg cagaccgggt
      781 ccgcctcttt ttctccgcct gtttcgtagc gataggagtc agcgacggcc ggccggcgta
      841 ttcgcgctca gcgccatcaa gcagccgttc gacacgcctg cgagccaatc gccgaaggtc
      901 ggcctgccag cgaagcaggg cacagggtga gcggctcgca tggccgatac ggcaaacgag
      961 tccgctacct cttcttcgtc ttcttttgaa aactcaggac tccatccagt tcctgcaggc
     1021 caccatcgtc ctcctgcgcc accgagccac tccacacctt cacgggttcg tctgggccgc
     1081 cgtccggctc gaaccacagc aacgagaacg tctcttcaaa ggtcgggtag tgcttcgcca
     1141 tctcggtcag gtcgtagcca ttgggccagt ctcgaaacca aacgtcctgc gcgacccgtg
     1201 agtcttcgcc tgaccactcg ccctcttgta caagccgatg ggcaaccgat ccgggcggga
     1261 tcggtgacct catctcgacc caggcattga ggtcgcgcag agccttggag cgcgatgcgt
     1321 accgtactac cgtcgcgttc atgaacacga aggcgcacgg atagtctgtg actgcggcta
     1381 ggcgcgaagc gcaggcagcg aaggagacca cgtacttcga gcgcaagcgt tcgatcaact
     1441 cgaacgaagg ttggtcctgg tccacgtcgc gcttgaacgc gtcgaacggc atcaacagtt
     1501 cggatgcaaa aatgtcgcac gcgatctcgt tgtcgtcgcg cttggcatag gaccaagctg
     1561 gggtttcttg atgattcgag gcaagggcca gcacgagatg gcccacctca tggcaagccg
     1621 agaatcgctg gcgctcacgc cgctccaact cgttgacgac gatcgacgac tttccgtccc
     1681 gacgggtgag cgtgtaaccg gactcccccg ggctcatctc ctcggtcgtg agcttggcat
     1741 tgactttccg ggtgtagaca gtcagatcct tgtcgatatt cgacagatcg agccctgcca
     1801 tgaactggcg cgccttcagg cggatcgcca gttcgtctat cactcgtcgt cctcgagtgc
     1861 cttacggacg cgcgctgcta tggcctccat atcgggcctt ccggccccgc ggtgccgcat
     1921 cgttgcgccc atctcgaaca tctctgccgt caggtcggtg ccaagcacat gctcgaccca
     1981 gtcggggttg acttcattgc tcatcatgaa ctgggccatc tcgacctccc gcggtttcgc
     2041 cttcagcacc cgcagtagcc gttccatcag ttcgctcgaa ggtgattgct tctccccagt
     2101 ctccaggcgg tagatgtagg tgtggtcgat gtcggcgagc tgaccgagct cccgcgatga
     2161 caggtctcgg gcctcgcgca actttcgcaa gagcatcccc aggccggact gcgccataag
     2221 ggcttccttc ctcagaaaaa tgcgcgtaaa gtgttgcctg tgcaggcaac aggcgccaag
     2281 atcacctaga gatcacagat cgttgcgtag actctgctcg ttgcccgcac aagcagcaac
     2341 tatgccactc aaccccctct tctgcaagca gatccattga taggagcgaa ccatggctaa
     2401 gaacaagcct atcggcgaca acgctcgtgt cggagcggtc cgtgaccgct cgcaagtctt
     2461 caatccgctg acgcagacct ggacgaaacg tgacgcttcg tctggccggt tcatggacca
     2521 gaaggctgac ggagatccgt tcaagggcgt ccgcaaggaa aaacctgtga agtaagcgcg
     2581 gcgggtcaag accgccttgt gtggtccccc gcccacccgt ggagaccata tgcatcggga
     2641 cttttccatc gaaccgcctc gccgccgctt ctcggttggc gcttcggcca gattcttcta
     2701 tctcggcgag gctatcgccc gcgtctacga gccaacgcag acgcagttga gcgacctgga
     2761 tcgcgcctat cagagcaccg gcgagtacct ggtgacctgc ccggagttcg acggcctgct
     2821 gtacctcgtg cacgcgcacg gctcgcgcca gttgggcacc atggtccggc cgatggaaga
     2881 gtaccgcgaa ggcttcgaca tcgacttggc cgcgtgcctg acccgtgaag gtctccagcg
     2941 atatggcggc gccggtggcc cggcgcggct catcaacgac ctctacgccg cgctcaagcg
     3001 gtacgccgac cgtcacaacc tgaagatcga gcgtgatgac cgctgcgtga ccatcaccta
     3061 cgccggcggc atgaaggccg acttcgcgcc catcattgat gacgcgtcgc acgttgtgct
     3121 gcatggcgag acgcatggac gcatccccga ccgtgacctg cagcgctaca tgtccacgaa
     3181 cccccgcggc tactgccgtg cgttcgactc gatcgcccag gttgcaccgg cctttgagct
     3241 tcgggttgag ctcaacgcca cgttcgagtc gctgcgcaag tccgaactcc tgcctctgcc
     3301 tgatgccgaa gaagtcttcg cccgtctgtt gtcgcgtctg acgcaactcg cgaagatcaa
     3361 ccgcaacatc gcattcggtg cacccacaag cggagagaac ttggcgccgc cgtcctgcgt
     3421 cgtgaccacc ctggtcgcga acgcctacgc gatcgaagcg ccgaagcccc acgacgggcc
     3481 gatggacctg ttgctcgaca tcgttcagtt gatgccgcga ctcatcaagc ggttcccgat
     3541 gcctgaaggc agcgagttct ggtatctcga caacccgacc gcccagaacg acaacctggc
     3601 tgccggtatg aacacgaagg cgaagcagca ggccttcgac gcgtggtgtg agcgcttggt
     3661 cgcggacctt acttccctcg tcgacgccat cgaccagggc gctgggatgg acgtcatcgc
     3721 tcgtgctgtc gagcgtgcct tcgggccgcg cgcgcgggca gcggtgctcg gggacaacgc
     3781 acagcggcgc gaagcgaacc gttccgcaaa tcgcggcatc ttcgtcgtgg ggtcgagtgc
     3841 cgtggtctcg acgccgacct tcgcgcacac gaacttcggt gactgaatgc gagcgacacc
     3901 gcccgtgcga ctcccaacgc tcgccgagcg ggcgatggaa ctgcgcgcgc tgaacttccc
     3961 tggcgcgcgc gtgcgactgc tccacggtcg cgagcttcgc tacagcttct cgatctcgcc
     4021 gacggtcttc tcccgtctgt atcggtgcgt gctcgtgcta acgccggcac agtcgccgca
     4081 gatgttcgtc gtgaaccctg acctgcgggc gctggccaag ggccagccgc tgcctcacat
     4141 ttaccactac gacggaccgg gcgtgaggct ttgcctgtgg ctaccgcgca agaacgaatg
     4201 gctgccgcag atgcgcttgc tggagaccta cgtggcctgg accagcgagt ggctgaacta
     4261 cttcgaggag tggcttgtca cgggggagtg ggcaggcggc ggggagcatc caccgccgcg
     4321 gaagaagcgc cgcctcctcc gcgcaactag accggtgacc acatgaacaa cattccccaa
     4381 aggcagttgc tgccggcgat gctcagcctg gttcgggcgc gcacgctggc ctatcgccgc
     4441 gccaagcgct gccaggcgtt cgttctcgcg ctcaatctgc tgctcccggt cgtatcggcg
     4501 gccgcggccg tcttcatgcc cgacgcccgt cccgccatcg cagtctgcgc cctgctcctg
     4561 ggcatcttcg acgtgaccgc gatcgacgca tggatgaagg cccgtctgaa ggtcggtgcg
     4621 aagctgcaag aagagttcga ttgcgaggtt ctcgacatcg actggaacga gttcgtggcc
     4681 ggcacgaaga tggacgcaga ggcaagctac gaggacggct gtcgaacgct cgatgacgtc
     4741 gacgagaggc gtctgcgtga ttggtacccg gtcgtggtgg ccgagacgtc tctggaagta
     4801 gcccgtgtca tctgccaacg agagaacctc gtctacgaca gcgacctgag ggtggcctac
     4861 cgccgcgtcc tcttctggtt tgtcgtcgtt ttcgttgttg tgttcagcgc ggcaagtttg
     4921 gccttcaatc cctccttcat gtcgcttgtg cttgcgctgg tgccggccgc gccggctctg
     4981 acctgggcgc tgcgcgaaaa caagcggcaa ggcgacaccc tcgagaccct gacgcggctg
     5041 aaggccgaat ccgaaaagct gctgaaggcc actatcaacg gcgtcgatgc tgccgaagcg
     5101 aagagacggt cccgtgaact gcaggatgcg atcttcggcc accgcctatc aagctcactc
     5161 gtctttgact ggctctacaa cctgctgcgc ccgcggctcg aagcaaagtt ggtccacggc
     5221 gcgaagcgtt gggtcgatga agccaagagg tcaggcactt gagagcgcgg taggacgctg
     5281 gcatcgcatg attgagcccg tggtctgccg ccactccaag tcaaccagca gcagcagcac
     5341 tcggcccgct ccacctgatc ggcggctgaa gactgactat ctcttcttgc ctaccttcgt
     5401 cgcgtccgtt tgattgcgac tctcgcgcgt tgaagcggcg ggctcgttca agttgtagaa
     5461 ctcatgcagc ttggctgcca gcatttgctt gtcgggcaat tgggtctggt attgcgcgac
     5521 gagagctggc gatagcgctc gactcagcgc gtactccact acctcgctgt ccttggaagc
     5581 acacagcagg acaccgatgg ccgggctctc gtgcggtttg cgcacgtcac ggtccagcgc
     5641 ttccaggtag aagttcagct tgcccaggtg ctcgggctcg aaacggtcca ccttaagctc
     5701 gatggccacc aagcagttca ggccgcgatg gaagaacagc aggtcgagcg cgaagtcgcg
     5761 tccgcctacc tgcagcggga actccgaccc cacgaagcaa aagtcgcggc cgagctcaat
     5821 caggaaggtc tgaagccggc caagcaagcc gcggtgcagg tcggcctcag tgtgtccgac
     5881 tggcagttcc agaaattcca gcgcgtaggc gtccttgaag acagtagccg ccgactcacc
     5941 gtggatttct ctcaccgctg gtgagagttt tgccggcgca agcaccgcct gctcaaaggc
     6001 tccgaggcgg atctgtcgct ccaactcgcg cttgccccag cgctgctgca cggccatgcg
     6061 caggtagaac tcgcgctcct cggcacgctt cgactggccc aggatgatga ggttatgggt
     6121 ccagggcaag gctctcacca gtggtgagag ctttggggcg tcgcggtagg tctcgaacag
     6181 ctgccgcatg cgccacaggt tctgggccga gaagccacgc agtcccggct ccgtgcgtgc
     6241 caggtgctgc gccagacgtt ccacaacgcc ctcgccccac tcagccgctg ccagcttgcc
     6301 gttgatgtac tcgccgatct gccagtacag gccgatgagc tcagtattta cagcctggtg
     6361 ggcgcgctgg cgcgcagcac gaatcagatc caccacttcg ccaaacgccg actcaacggg
     6421 cacgtccgcc gccgcgcccg agcgcgaatc gcgatgcgcc accgcggttt tcttggccgt
     6481 cttgcgaatg ctcatgccga ccattgtgcg tgaggcctgc cggcgcgcct cttgacttcg
     6541 cacccgcact ttgagtcgct ggacggctcg gagcggagcg tcgaatgcct cggtctcagg
     6601 cagcgacgac gcgcccgcct cctcgctcgc gcgcccaggc cgcagccagc cgctgcgcct
     6661 tgactaccgc agttcctccg tccagggcgg cggcgatccc cttccctcct accgccccat
     6721 gctcgcgcaa ccagaagatc atcttttcgg atgcgtcgag atcgcccagc gcgcggcaca
     6781 cggtggccac ggcttggcgg tccagggcca agaacgctcg ggggtagaaa agccgattgc
     6841 cgatcttcac ggcgaagacc tcgccccgac ctgcggccgg cctcaacgcc tgcctagtga
     6901 ggccccaggc tcgtgcgagt tgttcaccag ggacgacgag cccttccttg acccattgca
     6961 ccttggttgc ctccccacgc gcgagagcgc cggccaggcc ggttggaatc ggcgaccggg
     7021 ccacctgcgc tcgcaagccg ccggccaagc ttgaggcgaa cccgtggcgg ctgaccgcca
     7081 ttcgaaggct ctccaggtct tcggtcagcc gtcctacggc cgcttcgaga tgggcgatgc
     7141 gccgaagcag tacagcgggg ctggcgttgg ttcccatagg gcgtcttgca ggcaagagcg
     7201 atagcagaga aaacgtagca atcgtagcaa gcggcggtct gaaggcggcg acccccgaag
     7261 cgcagccggt ccaagcccga gagctgccgc gcccctttga cgcggcgcgg ctgcctttgg
     7321 tcggttcatg cccgatgaac gtgcgccggc gtggcgcgtt gtctttccct tccagcaccc
     7381 ttgttccata ctcgattcgc ttcgtggc
//
LOCUS       DAOORV010000162         7387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12368_length_7387_cov_8.264866, whole genome shotgun sequence.
ACCESSION   DAOORV010000162 DAOORV010000000
VERSION     DAOORV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12368_length_7387_cov_8.264866"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKV56_15680"
     CDS             <1..620
                     /locus_tag="PKV56_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HOX69301.1"
                     /translation="FLYIDEVNLLEDHLVDLLIDVAASGENVVEREGLSVRHPARFVL
                     VGSGNPEEGELRPQLLDRFGLSVEVKTPTDLASRVEVVRRRDQFERDPDGFTEKWKKD
                     DERVRRSIVKGKAHLADVTVPDAALERAAQLCMALGTDGLRGELTLIRAARSLAALQG
                     AKSVGDEHLKRVAPPALRHRLRRNPLDDAGSGVRVDRAVAELFGA"
     gene            617..2575
                     /locus_tag="PKV56_15685"
     CDS             617..2575
                     /locus_tag="PKV56_15685"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D"
                     /protein_id="HOX69302.1"
                     /translation="MTPAASTATAQELRWRCAQPALSIAARHAAALFAVDPVGTGGAV
                     IRGLPGPSRDGWLRHLAQLLPEGTPMRRVPLHINDERLLGGLDLAATLQAGRPVAQQG
                     VLTLADGGIVLLAMAERLPPSTAARVAAVLDTQEVALERDGLATRHATRFGVVALDEG
                     LADDEHVAPALLDRLAFSVDLHPVLVDAAGGPEDDLALEDDLFSLAGWSAADIASARA
                     LLPQVEAAAETVQALVSTAVALGVVSLRAPLLALRVARASAALDGRLRVSDDDAALAA
                     ALVLAPRATQVPTAPSEPEPNDDTDQPQSPEPEAQSEPPPEDTSEPPPPDDQDESADE
                     PEAEPSVADLEDRVLDAARAAIPAGLLAALMSGCDRRASAQVSGRAGAVQQGRKRGRP
                     SGARRGEPRSGARLNVIETLRAAAPWQKIRHAEAERLRPIHAKPGARALVQVRRDDFH
                     VTRYKQRSATTTLFVVDASGSSALNRLAEAKGAVELLLADCYVRRDQVAVLAFRGRGA
                     ELLLPPTRSLVRAKRSLAGLPGGGGTPLASGIDAAAALAASIKRRGETPIVVLLTDGR
                     GNIARDGSPGRVRAAEDVLLAARQFREAGLRALLIDTSPQPQPSSRQLADQMGASYLP
                     LPHAGSAALSQAVSLAARAASGPTAVAR"
     gene            2589..3530
                     /locus_tag="PKV56_15690"
     CDS             2589..3530
                     /locus_tag="PKV56_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX69303.1"
                     /translation="MSARLNWEQDGRDWPHREASQFVEAGGLRWHVQRFARTASASGV
                     PGPAPLALLLHGTGASSHSWHPMLPWLQPHFDLLLLDLPGHAYTSMPSAWQASLPGMA
                     QSVRALLDALDTTPTIIIGHSAGAALGARMCLDGHASPQSLISINGAFLPLGGLAGVL
                     FPPAAKLMAALPFAPGMVARRAADPRAIARLVSDTGSVLDEAGMALYGRIVSNPGHIE
                     GALAMMANWDVQPLARDLPRLQTPVALIVADNDRAVPPAQAQRVLALLPPQARTTLTR
                     LSGAGHIVHEEQPAQVAALCLLALRAQALPSRVVAAV"
     gene            complement(3562..4113)
                     /locus_tag="PKV56_15695"
     CDS             complement(3562..4113)
                     /locus_tag="PKV56_15695"
                     /inference="COORDINATES: protein motif:HMM:NF023010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HOX69304.1"
                     /translation="MKVQRFVKWLGVLASLLVQAAALPVQAQTTQIKTEYLMTYEVPL
                     DAPVPIDGSLLIVNVKPTGGWAKGPAINGTFLSPGADWLRVLPSGALRLDVRALLKTD
                     DGALIYVTYNGIVQNSPESAEKLNKGERLTAKDIPYFVTAPTFQTSSPKYAWLNSVQA
                     VSKMVEIKFGEGGFVRYDTFIVR"
     gene            complement(4156..5475)
                     /gene="ccmI"
                     /locus_tag="PKV56_15700"
     CDS             complement(4156..5475)
                     /gene="ccmI"
                     /locus_tag="PKV56_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HOX69305.1"
                     /translation="MTIFWTLVAALSVLALTLVLPVLIKRRPGAVPVSGASARLSTIA
                     ILRDQRSQLDAELEAGTLDAEQHRAGCEEIERRVLEEAEGSAGGVAPAAGVALGATPR
                     AWKTALGLGLGVPLLAIGIYGQLGAPQAIDARPAAVATSAADELTVAQVEKLVQTLAE
                     RMEAKPDDVQGWTLLARTYATMQRFAEADKAYSRATALAPQDAQLLIDHADVRAMLQG
                     QRLAGEPARMIARALELDPKNVKGLALAGGVAYEAGDNPQAVHYWKLARDLTPPGSEF
                     ADNLTKGIEQAQAGAPSDAGAPPAPSEGGAPAAAGAGGSATGPEASKAAASRITGQAS
                     LSPSLASKVAPTDTVFIFARAAEGPRMPLAIVRRTVADLPITFTLDGTMAMSPEMSLS
                     RYPVVVVGARVSKSGNAMPSPGDLTGQVANVKVGTEGLNIVIDTVQP"
     gene            complement(5472..5963)
                     /locus_tag="PKV56_15705"
     CDS             complement(5472..5963)
                     /locus_tag="PKV56_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOX69306.1"
                     /translation="MRRLRLIALVLISATWGVHAAPEAARSPQEAANSAADPALEARV
                     MKVAEELRCLVCQNETIAASHADLAVDLRKQIRTKLGEGQSERQILDFMVERYGDFVL
                     YRPALKATTVLLWVGPFALLLVAGFALTRAIRGRQRQAAAPLSPSEAARARQLLDESP
                     SGS"
     gene            complement(5960..6487)
                     /locus_tag="PKV56_15710"
     CDS             complement(5960..6487)
                     /locus_tag="PKV56_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOX69307.1"
                     /translation="MKRYLLPLLVFVLLAGFLAVGLKRDPREVPSPLIDKPAPAFSLP
                     QLDAPDQQVSPKDLLGQVWLLNVWASWCVACRQEHPLLVDVSKAGQVKLYGLNYKDKR
                     EDALGWLQQFGNPYLKSMSDTEGLVGIDYGVYGVPETFVIDKQGVIRYKQIGPVTPEA
                     LRDTLLPLVAKLEKS"
     gene            complement(6484..>7387)
                     /locus_tag="PKV56_15715"
     CDS             complement(6484..>7387)
                     /locus_tag="PKV56_15715"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOX69308.1"
                     /translation="LDMGKISVGPPYFVAVFVPLMAPALFLMGVGPMARWRQAGVIDL
                     GRRLRWAFGVSAASAVIAPLALGHWTPMIGFGLLLSAWIASTAVLNLVTRLSESKADY
                     LGGRFLAQSGSYYGMLLAHLGVSVFILGVTVVSGYQSESDVRMQVGETTSAGGYTFTM
                     QGVREVKGPNYVAAQASIGVTRDGQTVSTLWPERRIYNVTRSPMTEVAIDRSLARDLY
                     VALGEPVSPTAWSVRVHHKPLVNLIWLGCLLMAGGGVLAVMDRRYRVRRTSQANAPVP
                     TGSAGLPPRPMSIIGMIVRKFRTR"
BASE COUNT         1134 a         2516 c         2560 g         1177 t
ORIGIN      
        1 gcttcctcta catcgacgaa gtcaacctgc tcgaggacca cctggtcgac ctgctgatcg
       61 acgtggccgc ttcgggcgag aacgtcgtcg agcgtgaagg gctcagcgtc cggcatcccg
      121 cgcgcttcgt gctggtcggc agcggcaacc cggaagaagg cgaactgcgc ccgcaactgc
      181 tcgaccgctt cggcctgtcg gtcgaggtga agacgccgac cgatctggcc tcgcgggtcg
      241 aggtggtgcg gcgtcgcgac cagttcgagc gcgaccccga cggcttcacc gagaagtgga
      301 agaaggacga cgaacgcgtg cgtcgcagca tcgtcaaggg caaggcgcat ctggccgatg
      361 tcaccgtgcc cgatgcagcc ctggagcgcg cggcccagct ttgcatggcg ctgggcaccg
      421 acggactgcg aggcgagttg acgctgatcc gcgccgcccg ctcgctggcc gccttgcagg
      481 gcgccaagtc ggtgggcgac gagcacctga aacgggttgc gccgccggcc ttgcggcacc
      541 ggctgcgccg caatccgctc gacgatgcag gctccggcgt gcgcgtcgac cgggcggtgg
      601 ccgaactttt cggggcatga ccccggcggc atcgacggcc acggcgcagg aactgcgctg
      661 gcgctgcgcg cagccggccc tgtcgatcgc ggcccggcat gccgccgcgc tgttcgcggt
      721 cgatccggtc ggcaccgggg gtgcggtcat ccgggggttg ccgggaccgt cgcgcgacgg
      781 ctggctgcgc cacctcgcgc agttgctacc cgagggcacc ccgatgcggc gtgtaccgct
      841 gcacatcaac gacgaacgac tgctcggagg gctcgatctg gcagccacgc tgcaagccgg
      901 tcggccggtc gcgcagcagg gcgtgctgac gcttgccgat ggcggcatcg tgctgctggc
      961 gatggccgag cggctgccgc cgagtacggc ggcgcgggtg gcagcggtgc tcgacacgca
     1021 ggaagtcgcg ctcgaacgcg atggcctcgc gacccgtcac gccacccgct tcggcgtggt
     1081 ggctctcgac gagggactcg cggacgacga gcacgtcgcg cctgcgttgc tcgaccggct
     1141 ggccttttcg gtcgacctgc atcccgtgct ggtcgatgcc gcgggcggtc cggaagacga
     1201 cctggcgctc gaggacgacc tgttcagtct tgccggctgg agcgcagctg atatcgcttc
     1261 ggcgcgcgcc ttgctcccgc aggtcgaggc cgctgccgag accgtgcagg cgctggtgtc
     1321 gaccgcggtt gcactgggag tggtctcgtt gcgtgcccct ctgctggccc tgcgcgttgc
     1381 ccgtgcgtcg gcggcgctcg acggtcgcct gcgcgtcagc gatgacgacg ctgcgctggc
     1441 cgcggcgctg gtgctggcac cgcgcgccac gcaggtgccc acggcgccgt ccgaacccga
     1501 gccgaacgac gacaccgatc agccccaatc acccgagcct gaagcacagt ccgaaccgcc
     1561 accggaagac acctccgaac ctcccccgcc ggacgatcaa gatgaatccg ccgatgagcc
     1621 cgaggccgaa ccgtccgttg cggacctcga ggaccgcgtg ctcgacgcgg cccgggccgc
     1681 catccccgcc ggactgcttg cagccttgat gtccggctgc gaccgccggg cgagcgccca
     1741 ggtctctggc cgagcgggag ccgtgcagca gggccgcaag cgcggccgtc cgagcggtgc
     1801 acggcgcggc gagccacgtt ccggggcccg cttgaatgtc attgaaaccc tgcgagcggc
     1861 ggcgccctgg cagaagatcc ggcacgccga ggccgagcgg ttgcggccga ttcacgccaa
     1921 gccgggcgca cgtgcgctcg tgcaggtccg tcgagacgat ttccacgtca cccgctacaa
     1981 gcagcgcagt gcgacgacca ccttgttcgt cgtcgatgca tcgggttcgt cggcgctgaa
     2041 tcggctggcc gaggccaagg gtgctgtcga gttgctgctt gccgattgct atgtgcgtcg
     2101 cgatcaggtc gccgtgctgg cgtttcgggg ccgtggcgcc gagttgctgc tgccgccgac
     2161 acgatcgctg gtgcgtgcca aacgcagcct ggccgggctg cctggaggcg gtggaactcc
     2221 gctggcctcg gggatcgatg ccgccgccgc actggccgcg tcgatcaagc gacgtggcga
     2281 gacaccgatc gtcgtgctgc tgaccgatgg ccggggcaac atcgcccgcg acggcagtcc
     2341 cgggcgggtg cgtgccgccg aggacgtgct gctggcggcc cgtcagtttc gcgaggcggg
     2401 tctgcgcgcc ttgctgatag acacgtcgcc acagccccag ccttcgtccc ggcaactggc
     2461 cgaccagatg ggcgccagct acctgccgct gccgcatgcg ggatccgcgg cgttgtcgca
     2521 ggccgtttcc ctggctgctc gtgcggcgtc gggaccgact gcggtcgctc gatgagcggg
     2581 ggcggtctgt gtcggcacgc ctgaactggg agcaggacgg tcgcgactgg ccgcatcgcg
     2641 aggccagcca gttcgtcgag gccggcggac tgcgctggca cgtgcagcgg ttcgcccgca
     2701 ctgcaagcgc gtcaggcgtg ccgggacccg cgccgttggc gctgctgttg catggcaccg
     2761 gcgcatcgag ccattcgtgg cacccgatgc tgccatggct gcagccgcat ttcgatctgc
     2821 tgctgctcga cctgcccggg catgcctaca ccagcatgcc cagcgcatgg caggcctcgc
     2881 tgcccggcat ggcgcagtcg gtccgggccc tgctggatgc gctcgacacc acaccgacga
     2941 tcatcatcgg ccactccgca ggggccgcgc tcggggcgcg gatgtgtctc gatggacatg
     3001 catccccgca gtccctgatc agcatcaacg gcgccttcct gccactgggg ggtctcgccg
     3061 gcgtgctgtt cccgcccgca gcgaaactga tggcagcgct gccgttcgcg ccgggcatgg
     3121 tcgccagacg cgccgccgat ccccgcgcca tcgcgcgcct ggtcagcgac accgggtcgg
     3181 tgctggacga agcgggcatg gcgctgtacg ggcggatcgt cagcaatccc gggcacatcg
     3241 agggtgcgct cgcgatgatg gccaactggg atgtgcagcc gctggcacgc gacctcccgc
     3301 ggctgcagac accggtcgcc ttgatcgttg cagacaacga ccgggcggtg ccgcccgctc
     3361 aggcgcaacg ggtactcgca ttgctgccgc cgcaggcccg aaccacgctg acgcgactga
     3421 gcggcgcggg tcatatcgtt cacgaggaac aaccagccca ggtggctgcg ctgtgcctgt
     3481 tggcgctgcg tgcccaagcc ctgccgagtc gggtggtcgc ggccgtctga ggaccgcggg
     3541 tggtgaaccg cctggtgcgg ctcagcgcac gatgaaggtg tcgtacctga cgaagccgcc
     3601 ttcgccgaac ttgatctcga ccatcttgct caccgcctgc acgctgttga gccaggcgta
     3661 cttgggcgac gaggtctgga aggtcggggc ggtgacgaag taggggatgt ccttggcggt
     3721 gagccgctcc cccttgttca gcttctcggc gctctcgggc gagttctgga cgatcccgtt
     3781 gtaggtgacg tagatcaatg cgccgtcatc tgtcttcagc agggcgcgca cgtccaggcg
     3841 caacgcgccg gacggcagga cgcgcagcca gtcggcgccg ggcgaaagga aggtcccgtt
     3901 gatcgcgggg cccttggccc agcccccggt gggcttgacg ttgacgatca gcagcgagcc
     3961 gtcgattggc acaggcgcgt ccagaggcac ctcgtacgtc atcaggtact cggtcttgat
     4021 ctgcgtggtc tgcgcctgga ccggcaaggc ggcagcctgg accagcaggc ttgccagcac
     4081 gccgagccat ttgacgaaac gttgcacttt catggcaatt tccttatcga aactggctgg
     4141 atcccattcc atcgctcacg gttgcaccgt gtcgatgacg atgttcaaac cttcggtgcc
     4201 gaccttgaca ttcgcgacct ggccggtgag gtcgccggga ctgggcatcg cattgccgga
     4261 tttcgacacc cgcgcgccga ccacgacgac gggatacctt gacaggctca tttccggcga
     4321 catcgccatg gtgccgtcga gcgtgaaggt gatcggcagg tcggccacgg tgcggcgcac
     4381 gatcgccagc ggcatgcgcg ggccttcggc ggcgcgcgcg aagatgaaga cggtgtcggt
     4441 cggcgcgacc ttggacgcaa gcgaggggga cagactcgcc tgtccggtga tccgggaagc
     4501 cgcagccttg ctcgcctccg gtccggtggc cgaaccgccg gcaccggcgg ctgcaggcgc
     4561 gccaccttcg gaaggtgcag gcggtgcgcc ggcatccgag ggagcgcccg cctgggcctg
     4621 ttcaatcccc ttggtcaggt tgtcggcgaa ctcgctgccc gggggggtca gatcgcgcgc
     4681 cagtttccag taatggacgg cctgcgggtt gtcgcctgcc tcgtaggcca cgcccccggc
     4741 gagcgccagc cccttcacgt tcttcgggtc cagttcgaga gcccgggcga tcatgcgagc
     4801 gggctcaccg gccagacgct ggccctgcaa catggcacgc acatcggcgt gatcgatcag
     4861 cagctgggca tcctgcgggg ccagcgcggt ggcgcggctg taggccttgt cggcttcggc
     4921 gaagcgctgc atggtggcgt aggtgcgcgc cagaagggtc cacccctgga cgtcatccgg
     4981 cttggcctcc atgcgttcgg ccagcgtctg gacgagcttc tcgacctgcg cgactgtcaa
     5041 ctcgtcggca gcactggtcg ccaccgcagc gggacgcgcg tcgatggcct gcggtgcacc
     5101 cagctgtccg tagatgccga tggccaggag cggaacgccc agcccgagcc ccagggcggt
     5161 cttccaggct cgcggtgtcg cgccgagtgc caccccggcc gctggagcga cgccaccagc
     5221 cgagccctcg gcttcctcga gcacgcgtcg ttcgatctct tcgcagccgg cgcggtgctg
     5281 ctctgcgtcg agggtgccgg cctccagttc ggcgtcgagc tgggatcgct gatcacgcag
     5341 gatcgcgatc gtcgacaagc gggccgatgc cccactgacc ggaaccgcgc ccgggcgcct
     5401 cttgatcagg accggcaaaa cgagggtgag cgccagcacg ctcagtgcgg cgaccagagt
     5461 ccagaaaatc gtcatgagcc ggaggggctt tcgtcgagga gctgccgggc gcgggcggcc
     5521 tcggaggggc tgagtggtgc ggctgcctgt cgctggcgac cgcgtatggc gcgcgtcagg
     5581 gcgaagcccg cgacgagcag caatgcgaag ggacccaccc acagcagcac ggtggtggcc
     5641 ttcagggcgg ggcgatacag cacgaaatcg ccgtagcgtt cgaccatgaa gtcgaggatc
     5701 tgccgttcgg actgaccctc gccgagtttc gtgcgaatct gcttgcgcag atcgacggcc
     5761 agatcggcat gggacgcagc gatggtctcg ttctggcaga ccaggcagcg caattcttcg
     5821 gcgaccttca tcacccgtgc ctcgagtgca ggatcggcgg cgctgttggc agcctcctgc
     5881 ggggaacggg cagcttcggg agcggcatgc acgccccagg tcgcgctgat cagcaccagc
     5941 gcgatcagcc tgaggcgcct catgacttct ccagcttcgc caccagcggc agcagcgtgt
     6001 cgcgcagcgc ctcaggcgtc accgggccga tctgcttgta gcgaatgacc ccctgcttgt
     6061 cgatgacgaa ggtctcgggc acaccgtaca cgccgtaatc gatgccgacc agcccctcgg
     6121 tgtcagacat cgatttcaga tagggattgc cgaactgctg cagccagccg agtgcgtcct
     6181 cgcgcttgtc cttgtagttc agcccataga gcttgacctg acccgccttc gagacatcga
     6241 ccagcagcgg atgttcctgc ctgcaagcaa cgcaccacga tgcccagacg ttgagcagcc
     6301 agacctggcc cagcagatcc ttcggcgaca cctgctgatc gggagcgtcc agctgcggca
     6361 ggctgaacgc cggggcaggc ttgtcgatca gcggtgacgg cacttcgcga gggtcgcgct
     6421 tcaggccgac tgcgagaaag cctgcgagca gcacgaacac cagcaggggc agcagatatc
     6481 gtttcatcgg gtccggaact ttcgaacgat catgccgatg atggacatcg gccgtggcgg
     6541 aaggcccgcc gatccggtcg gcaccggagc attggcctgc gacgtgcgtc gaacgcggta
     6601 acgccggtcc atcactgcca acacgcctcc gccggccatc agcaggcagc cgagccagat
     6661 caggttgacc agcggcttgt gatgcacgcg cacgctccag gccgtcgggc tgaccggttc
     6721 gccgagagcc acgtacagat cgcgggccag actcctgtcg atggcgactt cagtcatcgg
     6781 tgagcgtgtc acgttgtaga tgcggcgctc gggccacagg gtgctcacgg tctgcccgtc
     6841 gcgggtgacg ccgatggaag cctgcgcggc cacgtagttg ggccccttga cttcacgcac
     6901 gccctgcatc gtgaacgtgt agccgccggc cgacgttgtt tcgccgacct gcatgcgaac
     6961 gtcgctttcg ctctggtagc cgctcaccac ggtgacacca agaatgaaca ccgatacccc
     7021 caggtgcgcg agcagcatgc cgtaatagct gccggactga gccaggaagc gcccgcccag
     7081 gtagtcggcc ttggactcgc tcaggcgtgt caccagattc agcacggcgg tgctggcgat
     7141 ccaggcagac agcagcaggc cgaagccgat catcggtgtc cagtgcccga gtgccagcgg
     7201 cgcgatcact gccgatgcgg cgctgacacc gaaggcccag cgcagccgac ggcccaggtc
     7261 gatgacaccc gcctgtcgcc atcgcgccat cgggcccacg cccatcagga acagcgctgg
     7321 cgccatcagc ggcacgaaca ccgcgacgaa gtacggcggg ccgaccgaga tcttgcccat
     7381 gtccagg
//
LOCUS       DAOORV010000163         7354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12441_length_7354_cov_5.965064, whole genome shotgun sequence.
ACCESSION   DAOORV010000163 DAOORV010000000
VERSION     DAOORV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_7354_cov_5.965064"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..2787)
                     /locus_tag="PKV56_15720"
     CDS             complement(157..2787)
                     /locus_tag="PKV56_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOX69309.1"
                     /translation="MTKIFLDIADRDPWSSAATASVSWAREQGVSLRDAVFLVPFAQH
                     LPLARQAWGDVETWMPRVETTQTLARSAAPPEPVAELDVCFDAALDDLTARRLLRGQA
                     AFAAWSRRDPQAFDQAANAVMQTAQAIARAISAVPPVHRAARGASIREMLGAVGGVGG
                     TERHLARIAFEWAAATIVQPTDVLFSLKPSAWIVVQAGGVDTLTQAIVDAAPGSCPVL
                     WIDADPAGDDVIRAMAAQARVQVQVCTDFEDEAQRAAAQVLADLSVHGAPVALIAQDR
                     LLVRRVRALLGRRAVPLRDETGWTLSTTRAGASVGLLLRAAAPAATVDDVLDAIKCWP
                     SSVVDPAAVASLEFTLRRRHWARPTAVDPDKLTSGAAALWELAAQATEPLRGAGAQPL
                     SGWCARVRAAMQASGLAAALARDDAGQQVLASMHLSGSSALEGQTEKLTLSDYARWVD
                     AALEQSTFVPASPEEPAVIVIPAPRAMLRRFTAIVWPGADEKHLGAAPSPHPLLNDVE
                     AAALGLSTAAERRQGEVLAFAQALRGAPVTLLRRTDDDGETVSPSPLLERLMSARRES
                     GLMPLGEAPDPRAVQVTSPTPVPRPLPSAPALLPDRLSASACEALRACPYRFFALRML
                     GLQPAEELDGEVEKRDYGTWLHEVLERFHRSRPPTMSAQDDALALALAAAAAQADMGL
                     SDGDFLPFAATFERFAPRYVDWLQARDQDGARWLDGEREWTAHPEAWRGISMHGVIDR
                     IDSIALAAGPVTQLIDYKTGSTSALRDKVKLRQEDTQLPFYAALAIEQGRRAGGIGPL
                     DAIYLSLDDSQKIVEVRHPEVEQSAEALVDGLGRDLARLAGGAVLPALGEGVACEHCD
                     ARGLCRRDQWSVIPATGATL"
     gene            2910..3239
                     /gene="trxA"
                     /locus_tag="PKV56_15725"
     CDS             2910..3239
                     /gene="trxA"
                     /locus_tag="PKV56_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HOX69310.1"
                     /translation="MSSELITHTSDASFDSDVIQSSKPVLVDYWAEWCGPCKAIAPIL
                     DEVSKAYDGRLQVTKMNVDDNRDVPAKYGIRGIPTLMLFKDGQLAATKVGALTKAQLT
                     AFIDGNL"
     gene            3466..4728
                     /gene="rho"
                     /locus_tag="PKV56_15730"
     CDS             3466..4728
                     /gene="rho"
                     /locus_tag="PKV56_15730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOX69311.1"
                     /translation="MHLSELKALHVSELITMGEALEIENVTRLRKQELMFAIMKKRAK
                     GGEQVFGDGVLEVLPDGFGFLRAPDASYMASTDDIYLSPSQIRRFNLHTGDDVEGEVR
                     VPKDGERYFALVKVDKVNGLTPEQSKNKIMFENLTPLFPKEQFKLERDIKSDENITAR
                     IVDLIAPIGKGQRALLVSAPKSGKTVMMQQLAHAIVANYPEAHLIVLLVDERPEEVTE
                     MQRTVRGEVISSTFDEPAQRHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTALVDTGSRMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSILLNKSGTRREELLLKPEILQKTWILRKLLYPMDE
                     IEAMEFILDKMKSTKTNHDFFDMMRRGG"
     gene            4845..5099
                     /locus_tag="PKV56_15735"
     CDS             4845..5099
                     /locus_tag="PKV56_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOX69312.1"
                     /translation="MKDGIHPNYRDVCFVDLSNGFKFVTRSCATTKETIKLDDGREVP
                     LFKLETTSESHPFYTGTQKSVDSLGGRVEKFRNKFAHLKK"
     gene            5157..6848
                     /locus_tag="PKV56_15740"
     CDS             5157..6848
                     /locus_tag="PKV56_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69313.1"
                     /translation="MINSSLNPALVSQRAVQRLPRWALLLFCAAYVLPGVFGRDPWRN
                     ADVTAFGYMISMVDGRSSWWMPAIGGVAADPSPLPYWLGAAFIRLLGPWVDPALAARI
                     PFAMLLVSVLLLTWHAAYHLARTEAAQPLPFAFGGEADPIDYARAMADGALLALIATL
                     GLLQLGHETTPELGQLACVALFIYALAASPFRGPRPKVALMISLPALAACGAPTIAVA
                     LGAIGIAVSARSNYPIVRRFAPWVAGATAATAAFSTGLDLWAWQVGNYRTLDQVLAVP
                     RQWLWFLWPAWPMALWTLWRWRRHVLNRHIAVPLGCAAVTAVASIVGGGQDRVLMLSL
                     PAWALLAAFALPTLKRSTAAAVDWFSVFFFSVCAIAFWVIYASMQIGFPVRPAQNVVK
                     LAPGFTPEFSLIALILATAGSLAWVWLVRWRTGRNQHAIWKSLVLPASGVALCWLLVM
                     TLMLPVLDYARSYRPLVDRVAMHVPQDACIATPRIQRSFQAALEYFGGFRVDASGSST
                     STECGYLLQVEPRKGKRPPAPSGWTRVARESRPTDRTEWMVIYGRQGRDKAATER"
     gene            complement(6888..>7354)
                     /locus_tag="PKV56_15745"
     CDS             complement(6888..>7354)
                     /locus_tag="PKV56_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69314.1"
                     /translation="STADNANDSIAGADQELHSAIANLVNNAIRYTPAGGSVHVLWSI
                     HSDGVGELEVRDTGIGIAKEHLPRLTERFYRVDGSRSRDTGGTGLGLSIVKHVAQRHG
                     GELEITSEPGQGSSFKLLFPAARVRSAAKSTDPAEGDASAPASVDNRHVDIT"
BASE COUNT         1315 a         2426 c         2334 g         1279 t
ORIGIN      
        1 ggatgcgtga caccagcatc caggtcttgc cggcgcccgc gcaggcttcg acgacaacgt
       61 gccggcgcgg atcgcaggcg acggcgtaga agtcctgcgc atcgacctgg gcgccgtcta
      121 tgagataggc aggccgcagc gcaacgttcg gcgcgttcac agggtcgccc ccgtggcggg
      181 gatcacggac cactgatccc gcctgcacag tcctcgggca tcgcagtgct cgcaggccac
      241 tccctcgccg agtgcgggca gcaccgcacc accagccagg cgcgcgaggt cccgtcccaa
      301 tccatccacc agcgcctcgg cactctgctc gacctcgggg tgacgcacct ccacgatctt
      361 ctgggagtca tcaagcgaca ggtagattgc atcgagcggg ccgatcccgc cggctcgcct
      421 gccctgctcg atggccagcg ccgcatagaa aggcagctgc gtgtcttcct ggcgcagctt
      481 gaccttgtcg cgcagggcac tcgtgctgcc ggtcttgtag tcgatcaact gcgtcaccgg
      541 gcctgccgca agggcgatgc tgtcgatgcg gtcgatcacg ccgtgcatcg agatgccccg
      601 ccaggcctcg ggatgagccg tccattcacg ttcgccatcc agccaccgcg cgccgtcttg
      661 atcgcgggcc tgcagccaat cgacatagcg tggcgcaaat cgctcgaacg tcgcggcgaa
      721 cggcaggaaa tcgccgtcgg aaagtcccat gtcggcctgc gcagcagcgg ccgccaaagc
      781 cagagcaagg gcgtcgtcct gcgcgctcat ggtcggcggg cgagaccggt gaaatcgctc
      841 cagaacctca tgcagccagg tgccgtagtc gcgcttctcg acttctccat cgagttcctc
      901 ggccggctgc agccccagca tgcgcagcgc gaagaatcgg tagggacagg ctcgcagagc
      961 ttcgcacgca ctggcgctca atcgatccgg cagaagtgcc ggagcagaag gcagcgggcg
     1021 tgggaccggc gtcggcgaag tgacttgcac agcacgcgga tccggcgcct cgccgagtgg
     1081 catcagcccg gattcacgcc gggcagacat cagccgctcg agcaaggggc tgggcgacac
     1141 ggtttcgcca tcgtcgtcgg tgcgccgcag cagtgtcacc ggagcgccgc gcaaggcctg
     1201 ggcgaatgcg agcacctcgc cttggcgccg ctcagccgcg gtcgacaagc cgagtgccgc
     1261 cgcctcgacg tcgttcagca gcggatgcgg cgagggtgca gcccccaggt gcttttcgtc
     1321 cgcccctggc cacacgatcg ctgtgaaccg tcgcagcatg gcgcgggggg ccgggatcac
     1381 gatcacggcc ggctcttcgg gtgaagcagg aacaaaggtt gattgctcga gagcagcgtc
     1441 gacccagcgt gcgtagtccg acagcgtgag cttctcggtc tgaccctcca gagcgctgct
     1501 accgctcaga tgcatcgagg caagcacctg ctgaccggcg tcatctcggg cgagcgcggc
     1561 tgccagcccg ctggcctgca tagctgcgcg gacgcgggcg caccagcctg acagaggctg
     1621 cgcgccagcc ccgcgcaacg gctcggtggc ctgcgcggca agttcccaca acgctgccgc
     1681 acctgaagtc agtttgtccg gatcgacggc ggtgggccga gcccaatggc ggcgacgcag
     1741 ggtgaactcg agcgaagcaa cagcggccgg atcgaccacc gatgaaggcc agcacttgat
     1801 cgcatcaagc acgtcgtcca ccgtggcagc cggtgcggca gcacgcagga gcaagccgac
     1861 gctcgcccct gcgcgcgtgg tcgaaagcgt ccatccggtt tcatcgcgca acggcacagc
     1921 gcgtcggccg agcagcgcgc gaacccgccg caccagcagg cgatcctgcg cgatcaacgc
     1981 caccggagcg ccatggacag acagatcggc aagcacctgc gcggcagcgc gctgcgcctc
     2041 gtcctcgaag tcagtgcaga cctgcacctg tacacgggcc tgtgcggcca tggcccggat
     2101 gacgtcatct ccggctggat cggcatcgat ccagagcacc gggcagctgc ccggagcggc
     2161 atcgacgatc gcctgcgtga gggtgtcgac cccgccggcc tggacaacga tccacgcgga
     2221 cggtttcagc gagaaaagta catccgtcgg ctgcacgatc gtggcagcgg cccactcgaa
     2281 tgcaatgcgt gcgagatgcc gctccgtgcc accgacgcct cccaccgcgc cgagcatctc
     2341 gcgaatcgat gcgccgcgcg ccgcccgatg aaccgggggc acggccgaga tcgcccgggc
     2401 gatcgcctgc gcggtctgca tcactgcgtt cgcggcctga tcgaaggcct gcggatcccg
     2461 gcgcgaccac gccgcaaagg ctgcctgtcc gcgcagcagg cgcctggcgg tcaggtcgtc
     2521 cagcgcggca tcgaagcaga cgtcgagttc cgccaccggc tcgggaggtg ctgcgctgcg
     2581 ggccagggtc tgggtcgttt cgacccgcgg catccatgtc tcgacgtccc cccaggcctg
     2641 gcgggcgagt gggaggtgct gtgcgaacgg aaccaggaac acagcgtcgc gcaggctcac
     2701 gccttgctca cgtgcccagg acaccgatgc ggtcgctgcg gacgaccagg ggtccctgtc
     2761 cgcgatatcg agaaagattt ttgtcatcgt ggcgatagaa aaggactcgt ctcgccagcc
     2821 tcgcaggacg caagccaaag cacccgatgt gccaaaatca ttgtgccttg cagcctacct
     2881 ccgggctcgc tcgtaccaag gacaacccca tgagcagcga actgatcaca cacacctccg
     2941 acgcctcgtt cgacagcgac gtcatccagt ccagcaagcc ggtgctggtg gattactggg
     3001 ccgaatggtg cggtccctgc aaagccatcg caccgatcct cgacgaggta tcgaaagcct
     3061 acgacggtcg tctgcaggtc acgaaaatga acgtcgacga caaccgtgac gtgccagcca
     3121 agtacggcat tcgtggtatc cccacgctga tgctgttcaa ggacgggcag ctcgcggcga
     3181 ccaaggtcgg cgccctcacg aaagcccagt tgacagcatt catcgacggc aacctataat
     3241 ctccggctgt cgggtggagt tctccgctcc accttgtcga tcgcccgcag tcccacgctc
     3301 ggattccggt agcccagtga ttccgccaga ccacaagtcg gcggccctcc cccacccgat
     3361 cgttgcgcga catgtcaatg tcggcgccga atcacggaag cacctgacag caggctgcgc
     3421 tcgtgcccgg atacacctgt tgatcatttt cccggtttcg cctccatgca cctgtctgaa
     3481 ctgaaagcgc tccacgtctc cgaactcatc acgatgggtg aggcgctgga gatcgagaac
     3541 gtcacgcgcc tgcgcaagca ggaactgatg ttcgcgatca tgaagaagcg cgccaagggc
     3601 ggtgaacagg tgttcggcga cggcgtgctt gaagtgcttc ccgacggatt cggcttcctg
     3661 cgcgcgccgg atgccagcta catggcgtcc accgacgaca tctacctcag tcccagccag
     3721 atccgccgct tcaacctgca caccggtgac gatgtcgaag gcgaagtgcg cgtacccaag
     3781 gatggcgaac gctacttcgc tctggtcaag gtcgacaagg tgaatggcct gacaccggag
     3841 cagagcaaga acaagatcat gttcgagaac ttgacgccgc tcttcccgaa ggagcagttc
     3901 aagcttgagc gtgacatcaa gtccgatgag aacatcaccg cgcgcatcgt cgacctgatc
     3961 gcacctatcg gcaagggcca gcgcgccctg ctggtgtctg cgccgaagag cggcaagacg
     4021 gtgatgatgc agcagctcgc acacgccatc gtcgccaact accccgaagc gcacctgatc
     4081 gtgctgctgg tcgatgaacg acccgaggaa gtgaccgaga tgcagcgcac cgtgcgcggc
     4141 gaggtcatca gctcgacctt cgatgaacca gcgcagcgtc acgtgcaggt ggccgagatg
     4201 gtgatcgaac gcgcgaagcg tctggtcgag atgaagaagg acgtggtgat cctgctcgac
     4261 tcgatcaccc ggctggcccg cgcctacaac aacgtgttgc catccagcgg caaggtgctg
     4321 accggcggcg tcgatgccaa cgccttgcag cggccgaagc gcttcttcgg tgcggcccgc
     4381 aacatcgagg agggtggctc gctgaccatc atcggcaccg cgctggtcga taccggaagc
     4441 cggatggacg aggtgatata cgaggagttc aagggcaccg gcaactgcga aatccacctc
     4501 gatcgacgca tggccgagaa gcgcgtgtat ccgtcgatcc tgctgaacaa gtcgggcacc
     4561 cgtcgtgaag agttgttgct gaagcctgag atcctccaga agacctggat cctgcgcaag
     4621 ctgctctatc cgatggacga aatcgaggcg atggagttca tcctggacaa gatgaagtcc
     4681 accaagacca accacgattt cttcgacatg atgcgtcgcg gcggttgagc ggtctctgcc
     4741 cgattgtctt gggctggatc gacctataat tcgaggtttc cctcacagtg gcaagtgcgc
     4801 cgaatggttc ggcgtggctc ccgcgacata gctcagaggt tccaatgaaa gacggcattc
     4861 accccaacta ccgcgacgtg tgcttcgtgg acctgtccaa cggattcaag ttcgtcacgc
     4921 gttcgtgcgc cacgacgaag gaaaccatca agcttgacga cggtcgcgaa gtgcccctgt
     4981 tcaagcttga aaccaccagc gaatcgcacc cgttctacac tggcacgcag aagagcgtcg
     5041 attctctcgg cggccgcgtc gagaagttcc gcaacaagtt tgcgcacttg aagaagtaac
     5101 gcggggcggc tgcgcctgca cgatcagaaa ggcagcctag gctgcctttt ttttggatga
     5161 tcaattcctc actgaacccc gccctggtgt cgcagcgcgc tgtgcagcgc ctcccgcgct
     5221 gggcactgct gctgttctgc gcagcgtatg ttctgccggg ggttttcgga cgcgatccct
     5281 ggcgcaatgc cgatgtgacg gcgttcggct acatgatcag catggtcgat ggccgttcat
     5341 cctggtggat gccagcgatc ggcggggtgg cagccgaccc gtccccgctt ccctactggc
     5401 tcggtgcggc attcatccga ctgctcggac cctgggtcga tccggctctg gcggcacgta
     5461 ttccgttcgc catgctgctg gtgtcggtgc tgctgctgac ctggcacgcc gcataccatc
     5521 tggcgcgcac tgaagccgct cagcccctgc cgttcgcctt cggaggcgaa gccgacccca
     5581 tcgactatgc aagggccatg gccgatggtg cgctgctcgc gctgatcgcc acgctgggtc
     5641 tgctgcaact cggacacgag accacgcctg aactcgggca actggcttgc gtggcactgt
     5701 tcatctacgc attggctgcc agtccgttcc gcggtccgcg acccaaggtc gccttgatga
     5761 tcagcctgcc cgcgctcgcc gcctgtggcg cgcccacgat cgccgtggcg ttgggtgcga
     5821 taggcattgc ggtgagcgcc agatccaact atccgatcgt gcgccgcttc gcgccctggg
     5881 tcgctggtgc aactgccgcg acggccgctt tctcgaccgg actcgacctc tgggcatggc
     5941 aggtgggcaa ctaccgcact cttgatcagg tgctggcggt tccccggcag tggctgtggt
     6001 tcctctggcc ggcttggccg atggcactgt ggacgctgtg gcgttggcgg cgccacgtcc
     6061 tcaaccggca catcgccgtt cccctgggtt gcgcggctgt caccgccgtc gccagcatcg
     6121 tgggaggtgg acaggaccgg gtgctgatgc tgtcgcttcc cgcctgggca ctgctggcgg
     6181 cgtttgcatt gccgacactc aagcgcagca cggcggcagc agtcgactgg ttctccgtgt
     6241 tctttttcag tgtctgcgca atcgcgttct gggtcatcta cgcgtcgatg cagatcggct
     6301 ttccggtgcg tccggctcag aacgtcgtga agctggctcc gggattcacg ccagagttct
     6361 cattgattgc actcattctg gccaccgcgg gaagtctggc ctgggtctgg ctggttcgat
     6421 ggcgaaccgg ccgcaaccag cacgcgatat ggaaaagcct ggtactgccg gcgagtggtg
     6481 tggccctgtg ctggctgctg gtgatgacgc tcatgttgcc cgtgctggac tacgcgcgca
     6541 gctaccgacc gctggtcgac cgcgtggcga tgcacgtgcc ccaggatgcc tgcatcgcca
     6601 cccctcgcat tcagcgcagc ttccaggccg cactggaata cttcggcggt ttccgtgtcg
     6661 acgccagcgg cagttcgacc agcaccgagt gtggctacct gctgcaagtc gagccccgca
     6721 agggcaagcg gccgccggca ccttctggct ggactcgcgt ggcgcgagaa tcccgaccca
     6781 ccgaccgcac ggagtggatg gtgatctacg gccgacaggg acgcgacaag gcggcgactg
     6841 agcgttgagc tgctccagtc acaacgccgt tccagaccgc cggcaattca ggtgatgtca
     6901 acgtggcggt tgtcgaccga agccggcgcg ctggcatcgc cttccgcggg atccgtcgat
     6961 ttcgctgcgg atctcacccg tgcagcgggg aacaacagct tgaagctgga gccctgaccg
     7021 ggttcgctgg tgatctcgag ttcgccgcca tgtcgctgcg cgacatgctt gacgatggac
     7081 aacccgaggc ccgtgccgcc cgtgtctcgc gaacggctgc catcaacgcg gtagaagcgt
     7141 tccgtcagcc gcggcaggtg ttccttggcg atgccgatac cggtgtcgcg aacttcgagc
     7201 tcacccacac catcgctgtg tatcgaccac agcacgtgca cggagccgcc ggcaggtgtg
     7261 tagcgtatgg cgttgttgac caggttggcg atggcactgt gcagttcctg atcggcaccg
     7321 gcaattgaat cgttggcgtt gtctgccgtc gaca
//
LOCUS       DAOORV010000164         7317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12517_length_7317_cov_7.497934, whole genome shotgun sequence.
ACCESSION   DAOORV010000164 DAOORV010000000
VERSION     DAOORV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12517_length_7317_cov_7.497934"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKV56_15750"
     CDS             <1..671
                     /locus_tag="PKV56_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69315.1"
                     /translation="GRVRALVLELARPADWQQLADVWKGVQTELDLPAPAIAVNGTDG
                     MQLWFSAEAPLDVARAHAFLDGLRLRYLAAVKPDRVSLMAGADSAWAASIGLSSSASA
                     PLETNGCWPAFVAPDLAPIFSETPWLDLPPGEEGQTRLLASVKSIKPELFDAALARLR
                     AWAAAEAPAAVAMGAGPRDGGSSAACHPDAKRFLLSVMNDEAAPLALRIEAAKALLPH
                     GQTH"
     gene            1000..1302
                     /locus_tag="PKV56_15755"
     CDS             1000..1302
                     /locus_tag="PKV56_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69316.1"
                     /translation="MTDYSKQLVDGENVVDHAAHSAEKLLEATRQNANSAIDGAADKV
                     QALRNRTSPALDRLMAPVDSLIERTRAAPLTSLLMAAAAGATLMALAGLLRPSRRR"
     gene            1395..2117
                     /locus_tag="PKV56_15760"
     CDS             1395..2117
                     /locus_tag="PKV56_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOX69317.1"
                     /translation="MIESLLLTTARVSTFFGTQPLTSASGFFFERDKRLYLVTNRHVL
                     IDRPSGHLPDRVEIEVHADADNLTHASVLSVLLYRDGQAVWHQGRDSAGDIDVAVVEL
                     DRAALPSTVMLSAFTPAHLQHRLQEVKVGTPLLVVGFPLGFHDTLHHLPVVRQAVISS
                     SFGLPFQGQGYFLTDARTHRGTSGAPVVMHDDAGDVQLPWKLLGVHSARMEMSNRGLP
                     VEESLGLNCAWYADILLTLTAV"
     gene            complement(2257..2670)
                     /locus_tag="PKV56_15765"
                     /pseudo
     CDS             complement(2257..2670)
                     /locus_tag="PKV56_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2710..2859)
                     /locus_tag="PKV56_15770"
     CDS             complement(2710..2859)
                     /locus_tag="PKV56_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HOX69318.1"
                     /translation="MLYTLAVVLLILWALGLVTSYTLGGFIHILLVIAVVMVLINIIG
                     GRRSA"
     gene            complement(3281..4441)
                     /locus_tag="PKV56_15775"
     CDS             complement(3281..4441)
                     /locus_tag="PKV56_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOX69319.1"
                     /translation="MRPLRPDRSHSPREHALLRALALIAAFLPAFAAAQAVGQADARD
                     GKALTLELCAEIGASSDRLICYDKLAGRAPAPTEPVAAQAGPGATAVPTTSRLAAKDA
                     PLPASAHPRPDNGILSRYWELEPSDKRGIFNFVGYHPNYVLPLHYSNRINRSPRSPTQ
                     AGVAKPDYRHEEAKFQFSLRTKVIQDVPLLGGDFWVAFTQQAMWQIYSKADSKPFRNI
                     DYQPEAIYVLPTAPGLRDLPFGWRWSYTQLGIAHQSNGQSDPLSRSWNRVYIGAGIER
                     GDWSLTAKLNQRLKESIDTDNNPDLVTYRGRAEFQLNWTQGLHTASLLYRSTLKNARY
                     GALQAEWSYPIFADHPNGLRWFVQGFSGYGETLTDYNFRQTSLGVGVTFLQF"
     gene            complement(4913..5000)
                     /locus_tag="PKV56_15780"
     tRNA            complement(4913..5000)
                     /locus_tag="PKV56_15780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4964..4966),aa:Ser,seq:cga)
     gene            complement(5080..6387)
                     /gene="serS"
                     /locus_tag="PKV56_15785"
     CDS             complement(5080..6387)
                     /gene="serS"
                     /locus_tag="PKV56_15785"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOX69320.1"
                     /translation="MLDVALLRKDLTSVIARLERRKSPQPFLDVDRFQSLESERKSLQ
                     TRTEELQAQRNSLSKQIGALKGKGEDTSAVMAQVAGLADELKSSAERLEVIQSELSDL
                     LMAVPNLPQDSVPVGADESGNVEVRRWSPHAGGPRSFDFTPKDHVDLGAPLGLDFDIG
                     AKLSGSRFSFMRGPVARLHRALAQFMLDVQTQEHGYTECYTPYIVNRDVLEGTGQLPK
                     FKEDMFWVSRGGDEVQQEQYLISTSEISLTNSVREQVLGADQLPIKLTAHSPCFRSEA
                     GSAGRDTRGLIRQHQFDKVEMVQIVQPERSDEALEEMVGHAEAVLQKLKLPYRVLLLC
                     TGDMGFGSSKTFDLEVWVPAQNTYREISSCSNCEAFQARRMQSRYKSAQGRNEFVHTL
                     NGSGLAVGRALVAVLENYQNADGSITVPDVLRPYMGGLEVLKA"
     gene            complement(6467..>7317)
                     /gene="ispH"
                     /locus_tag="PKV56_15790"
                     /gene_synonym="lytB"
     CDS             complement(6467..>7317)
                     /gene="ispH"
                     /locus_tag="PKV56_15790"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOX69321.1"
                     /translation="IYVRHEIVHNTYVVNDLKTKGAIFIEDLAEVPAGATLVFSAHGV
                     SQAIRREASERGFTIFDATCPLVTKVHVEMAKLRKEGYDFIMIGHKGHPEVEGTVGQL
                     TEGIHLVEDVADVEQVPVDSSTRLAVVTQTTLSVDDAAEILAAVKRRFPNVREPKQQD
                     ICYATQNRQDAVKVLAPQVDVMVVVGSLSSSNSNRLREVAERLGTDAYMVDSADDLKP
                     EWFAGKSRVGLTAGASAPEILVTQVIERLRALGAVSVRSTPGVLETVHFPLPMGLGDK
                     SMKASG"
BASE COUNT         1292 a         2350 c         2382 g         1293 t
ORIGIN      
        1 aaggtcgcgt gcgtgccctg gtgctggagc tggctcgtcc ggccgactgg cagcaactgg
       61 ccgacgtctg gaaaggcgtg cagaccgagc tcgaccttcc ggcacccgcc attgcggtga
      121 acgggacgga cggcatgcag ctgtggttct ctgcggaggc gccgctggac gtggcgcggg
      181 ctcatgcctt cctggacggc ctgcgcctgc gctacctggc ggctgtgaag ccggaccgcg
      241 tcagcctgat ggccggtgct gattcggcct gggctgcttc catcgggctg agcagttcag
      301 cgtcggcgcc gctggagacg aacggctgct ggccggcttt cgtcgcgccg gacctggccc
      361 cgatcttctc ggaaacaccc tggctggact tgccgcccgg cgaggagggt cagacacgtc
      421 tgctcgccag cgtgaagagc atcaagcccg agctgttcga tgccgcgctg gcacgactgc
      481 gggcatgggc ggcggcagag gctccagctg cggtggcgat gggcgcgggc ccgcgcgacg
      541 gtggctcgtc tgccgcctgt catcccgatg cgaagcgctt cctgctttct gtcatgaacg
      601 acgaggctgc gccgcttgct ctgcgcatcg aggccgcgaa ggcgctgcta ccgcacggac
      661 agacgcattg acctcccagc agttgggcgt cgcgtaccgg agttcgctgt cgatctccat
      721 gtggtcgcgc ggaagacatc ggcatcgcgg tgacggggcc attggctcgc tgcagcgtcg
      781 gtgactcggc cattggctga gcgcgtctca tggtcgctgt cactcggacg tcacgcggac
      841 tcgatcggca cgggacgagt tcgattgcgt gtgtggatgt atctggttcg cgcctgcgcc
      901 tacaaacgtt cagcgaccct gccgactcag gttcgcctcc gacccgacga cgatcgttca
      961 gcggccgatg ctcttcgctg caatgacccg aggactgaaa tgactgacta ttcgaaacaa
     1021 cttgtcgacg gcgagaacgt ggtcgaccat gccgcgcact ctgcggagaa gctgctcgag
     1081 gccacgcgac agaatgcgaa ctccgcgatc gacggcgctg ccgacaaggt tcaggcgttg
     1141 cgcaatcgca cctcaccggc gctcgatcgc ctgatggcac cggttgattc gcttatcgag
     1201 cgaacccggg cagcaccact gacaagcttg ttgatggcag ccgccgccgg agccaccttg
     1261 atggcactgg ccgggctgct ccgcccctct cgccgccgat aggacattcg gtcagccgac
     1321 cctggaacaa tcaagtggcg tcgctcagga tcgcaacagc ccagtcgcgg gccgaaccgc
     1381 tcccccactc cgccatgatc gagtcccttc tgctgaccac cgctcgcgtt tccaccttct
     1441 tcggcacgca gccgctgacc agtgcgagcg gattcttctt cgaacgcgac aagcgtctct
     1501 atctggtcac caaccggcac gtgctgatcg accgaccaag cggtcatctg cccgaccggg
     1561 tcgagatcga ggtgcatgcc gacgccgaca acctcacgca cgcgtctgtt ctgtcggttc
     1621 ttctgtatcg cgacggacag gccgtctggc accagggacg cgacagcgcg ggcgacatcg
     1681 acgtcgcagt ggtcgaactc gaccgggcag cgctgccatc caccgtgatg ctgagtgcct
     1741 tcacgccggc gcacctgcag caccggctcc aggaggtcaa ggtcggcacg ccgttgctgg
     1801 tggtcggatt tccgctgggc ttccatgaca cgctgcacca tctgccagtg gtgcggcagg
     1861 ccgtcatttc atcgtcgttc ggcctgcctt tccagggaca gggctacttc ctgaccgatg
     1921 cacgcaccca ccgcggcacc agcggtgcgc ccgtggtgat gcatgacgac gccggagatg
     1981 tgcagttgcc ctggaagctg ctgggtgtgc actccgcgcg catggagatg agcaaccggg
     2041 ggcttccggt cgaggagtct ctgggcctga actgcgcttg gtacgcggac atcttgctca
     2101 ccctcactgc cgtgtaatgc tccagaagcg gtgccccggg gacttgttgc atgcgaacgg
     2161 cagcaacctg ccgaccaggc aacgctgacc agacgccgtg gctgcagccg ccagacgatg
     2221 tctcgtgcca gatgccagag aaaggtgggg ccgggttcaa tgcaagccac cagcttcggg
     2281 cggcgcccca tcgggttggc cgtcaaagaa cgcgtagccc ggaaggccac gcctggctcg
     2341 atacatcgcg gcatctgcgc gccacagcaa ggtgagagcg gtatcgccat cgttcggaca
     2401 tagcgcaaca ccgatgctcg gccaccccct gacttctatt ccacccacct gcagcggcgc
     2461 cgctatgaca tcaagcaact cagtggctag gctgcctggt tgggcatgac catcgggctg
     2521 cgaaagcagg caagcgaact cgtcgcccac ttcgtggccg cgcaaaccgt cgatgggctt
     2581 gaagtcgtcc aggtccagga actgcacggc catcgtcggc gggcgcagca gatcggtgca
     2641 gcgtgcgtca tccagcctgc ggcggaactg tcgccgattg ggccgcgcgg caaaaagcgg
     2701 gcgtccgggt caagcgctgc gacgtccgcc gatgatgttg atcaggacca tgacaaccgc
     2761 gatcaccagg aggatgtgaa tgaaaccgcc cagggtgtag gaggtcacca accccagtgc
     2821 ccagaggatc aacagaacga cagcaagtgt gtaaagcatg atgggcccct ttcatcagga
     2881 gcgcgacacc ggcggtgcac gcgatcccag gacatgccgc aaatctgatc gccggacagc
     2941 tcagcgtctg tgcgatggcc cacggaaagc ttggagtgag cgtctgaagt tggatcaggg
     3001 gcgttgagga tcgcggggaa tcaacccacc cgatcgatcg gatctgagcc catcgggaac
     3061 ccggcgacac caggtttgtc gtgcatcgcc tgcccgcctt acttgatcac cgagacgtca
     3121 tcttgcgggt tggttcgacg cgatccccaa cagcccaggc gcaaaggacg aggagtccat
     3181 tgcggccact gagagtggcc cgcatgaaga ggccacgccg gctcgttcag ctcctctcga
     3241 cgatgcgcca cagcatttgc aggcgacgat catgcccctg tcagaactgc aggaacgtga
     3301 cgccgacgcc cagactggtc tggcggaagt tgtagtcggt cagcgtctcg ccgtagccgc
     3361 tgaagccctg cacgaaccaa cgcaagccgt tcgggtgatc cgcgaagatg ggataggacc
     3421 actcggcctg cagcgcgccg tacctcgcgt tcttcagggt gctgcggtac agcagagagg
     3481 ccgtgtgcag gccttgtgtc cagttcagct ggaactccgc gcggccgcgg taggtgacca
     3541 ggtcagggtt gttgtcggta tcgatcgact ccttcagacg ctggttgagc ttggccgtca
     3601 gcgaccagtc gccacgctcg atgccggcac cgatgtagac gcggttccaa ctgcgcgaaa
     3661 gcgggtcgga ctgcccgttc gactggtgcg cgatgccgag ctgggtatag ctccagcgcc
     3721 atccgaacgg caggtcgcgc aggcccggcg cggtgggcag tacgtagatc gcctcgggct
     3781 ggtagtcgat gttgcggaag ggcttggaat cggctttgct gtagatctgc cacatcgcct
     3841 gctgcgtgaa cgcgacccag aagtcgccgc ccagcagcgg tacatcctga atgaccttgg
     3901 tacgcaacga gaactggaac ttggcctcct cgtggcggta atcgggcttc gccacgcccg
     3961 cctgcgtcgg cgaccgcggc gagcggttga tgcgattgga gtagtgcagc ggcagcacgt
     4021 agttcgggtg gtagccgacg aagttgaaga tgccgcgctt gtcggagggt tccaactccc
     4081 agtatctgga caggatcccg ttgtcgggtc ggggatgggc gctggcgggc agcggtgcgt
     4141 ccttcgctgc gagccgtgaa gtggtcggca ccgctgtggc gccgggcccg gcttgcgcgg
     4201 cgacaggctc agtcggcgca ggcgcgcggc cggcgagctt gtcgtagcag atcaggcggt
     4261 cggaggaggc tccgatctcg gcgcagagct cgagcgtgag ggccttccca tcccgcgcgt
     4321 cagcttggcc gacggcctgc gccgcagcga aagcggggag gaaggcggca atcagcgcaa
     4381 gcgcgcgcag caacgcgtgt tcgcgaggcg agtggcttcg gtctggtcgc agaggacgca
     4441 tggcaaaggg gttgccggaa aatgttccag aggcaacgcg agtcggacgg ccgtttgatc
     4501 ctatgagctc ggcgcccgtc gccgtgtagg cggacgccgc gtccgcgctg gtgagagagc
     4561 ctgaagatcg agagcgatcg ccccagagcc gaagacaacc cgcctagcgc cacaaggatc
     4621 ggtcacgaca tgcctgacgg aaacgtcgct gccgattgca tgaaggtctc ccgtcgagtt
     4681 gagggccaac caagatgcac ctctgcgctt ctggatgcaa gcggcaggtg gatgccatgc
     4741 ggcccaacgg taaacagcgc ggaacaccat cgtcgagcct gaggaggccg ggctgaaaat
     4801 cgcagtgatg gtgccgcgca cagccaacag agaaagctgc accgcccgca tgcccgcgtc
     4861 gcgcgggctt cgccaaagaa aaagccaccg cgctgcggtg gcttttaaat gttggcggag
     4921 agggtgggat tcgaacccac ggtaccttgc ggtacgcctg atttcgagtc aggtacattc
     4981 gaccactctg ccacctctcc agcacgttcg atgctgccgg ttggtcggaa ccggaaccca
     5041 agattctatc tcagggagcc gcctcaggcg acccggcaac tacgctttca acacctccag
     5101 gccgcccatg tagggacgca gcacgtccgg cacggtgatg gatccgtcgg cgttctgata
     5161 gttttccagc acggcgacca gggcccggcc gacggccaga cccgaaccat tcagcgtgtg
     5221 gacgaactcg ttgcgaccct gcgccgattt gtagcgcgac tgcatgcgac gggcctggaa
     5281 ggcttcgcag ttggagcacg aactgatctc gcgataggtg ttctgcgccg gcacccacac
     5341 ctcgaggtcg aaggtcttgc tcgagccgaa gcccatgtcg ccggtgcaca gcagcaacac
     5401 gcggtacggc agcttcagtt tctgcagcac ggcttcggca tggccgacca tttcctcgag
     5461 cgcctcgtcg ctgcgttcgg gctggacgat ctgcaccatc tcgaccttgt cgaactgatg
     5521 ctgtcggatc agaccacggg tgtcacgccc cgcactgccc gcctcggagc ggaagcaggg
     5581 gctgtgggcg gtcagcttga tcggcaactg gtccgcgccc agcacctgct cgcgcacgct
     5641 gttggtcaac gagatctccg acgtggagat caggtactgc tcctgctgca cctcgtcgcc
     5701 gccacgggaa acccagaaca tgtcttcctt gaacttgggc aactgcccgg tgccctcaag
     5761 cacgtcgcga ttgacgatgt agggcgtgta gcactcggtg tagccgtgct cctgcgtctg
     5821 cacatcgagc atgaactgcg cgagcgcgcg gtgcagccga gccaccgggc cgcgcatgaa
     5881 gctgaagcgc gatccggaca gcttggcgcc gatatcgaaa tcaaggccca gcggggcacc
     5941 cagatcgacg tgatccttgg gcgtgaagtc gaagctgcgc ggacctccgg catgcgggct
     6001 ccagcggcgc acctcgacgt tgcccgactc atcggcaccg accggcacgc tgtcctgcgg
     6061 caggttggga accgccatca gcagatcgga cagctcggac tggatcacct ccagccgctc
     6121 ggctgaagat ttcagttcat cggccagacc ggccacctgt gccatcaccg cactggtgtc
     6181 ctcgcccttg cccttgagcg cgcctatctg cttggacagg ctgttgcgct gagcctgcag
     6241 ttcctcggtg cgggtctgca gcgacttgcg ctcggactcc agcgactgga agcgatcgac
     6301 atccaggaaa ggttggggag atttgcgcct ctcgaggcgg gcaatgacgc tggtcaggtc
     6361 tttgcgtagc agggcaacgt ccagcatggg tgtgcagtcg atcccggtcg acttgagtgt
     6421 tgtgcgtgaa gcggcgaatt gtaggcagcg acctgcccgt tgcgactcag cccgaagcct
     6481 tcatcgactt gtcgcccaga cccatgggca gagggaagtg cacggtttcc agcacacccg
     6541 gtgtgctgcg caccgacacg gcgccgagtg cgcgcaaccg ctcgatgacc tgcgtgacca
     6601 ggatctccgg ggccgacgcg cccgcggtca ggccgacccg gctcttgcct gcgaaccact
     6661 cgggtttcag gtcgtccgcc gaatcgacca tgtacgcgtc ggtgcccagg cgttcggcca
     6721 cctcgcgcag gcggttgctg ttcgaactgc tcaggctgcc gaccaccacc atcacgtcga
     6781 cctggggggc cagcaccttg acagcatcct ggcggttctg cgtggcgtag cagatgtcct
     6841 gctgcttggg ctcgcgcacg ttcggaaacc gccgcttcac cgcagccagg atctcggccg
     6901 catcgtccac agacaaggtg gtctgcgtca ccacggccaa gcgggtcgac gagtccaccg
     6961 gcacctgctc gacatcggcc acgtcctcga ccagatggat gccttcggtc aactggccca
     7021 ccgtgccctc gacttcggga tggcccttgt ggccgatcat gatgaagtcg tagccctcct
     7081 tgcgcagctt ggccatctcg acatgcacct tggtcaccag cgggcaggtc gcgtcgaaga
     7141 tcgtgaatcc gcgctcggat gcttcacggc gaatggcctg cgaaacaccg tgcgcgctga
     7201 agaccagcgt cgcgccagca ggcacctcgg ccaggtcctc gatgaagatc gctcccttgg
     7261 tcttcaggtc gttgaccacg taggtgttgt gcacgatctc gtggcgcacg tagatcg
//
LOCUS       DAOORV010000165         7282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12592_length_7282_cov_10.641068, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000165 DAOORV010000000
VERSION     DAOORV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12592_length_7282_cov_10.641068"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4337)
                     /locus_tag="PKV56_15795"
     CDS             complement(<1..4337)
                     /locus_tag="PKV56_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69322.1"
                     /translation="MARSDVRAFDRGAPDWLRRIVDDPLKLPFGRRLAKALGLRPNGE
                     GAAIALQVAAHVFEVHATHAELDMLANWADLASSMAFNARLATDFPRLLLDANAQEGE
                     RSWSALARLSKARFDALEVGVCVSLSLALLEAIRAKEGLPEALLPRVSSMAYAHGTEV
                     RRASREAWTLLAQLVSSPLNQETLDRLAEFAAGKQLDDARALASHLHAAQTSLRKVPD
                     PGTDTLVDDVEGDDGRQDGLLSEAGTVMAMVLAPGPSQPRADVTPRASCLPPSVRSLP
                     KAASFATMAELFAIQSAWNRQPPQRLKAVTAAAQLTLVGETVATNFEASLLGVMALVI
                     SHDFNPTLELSLENNGDLWYSRQKRCVLAHRRLLLKLDDNQPGPTWYPLYTPSAAADA
                     IESLWTAKPQARRFKDLFDPATLYDLVSQAEAWIKSLSDPAHPAWSARMAHSLGLVYL
                     ACGASPLEAGLQSLNTSLAPASAANYYQPNPARQHELASRVFQFLGLGTPGPIVYPEP
                     DDLPAAPDDEEVRAEWRHYCSNANAAFGEIGIASTQDAVVNALNKGMAAGRRAFKLLT
                     GGRAQQRTQPTLRDVCASDQWLRLDDKRTKPSSERLIPLTPAVREVIRVVWALRIAAR
                     DRLRALGVEDAAIPVLLIDPRPSSMLFLRLRTMDRRGRQILATGPLDADAMALVPQLW
                     RGPQNMGRRFWVTQAACSPQWWAELVITGHGRGLAHVGSPCLSLPIHCLLEGTRELCD
                     TVLSRLSLGAFGDAIPGEYPEVAVGVDLRHIDRRRNLNSRASDQPRHHCDSHTLPAIR
                     VIDALREHTGTSELLSPWARALLSLVVVDGLVHSADVRAAWECLRAVPVDPLEAGRTA
                     MLRFTRDSGQPICMPVQPPTRLARDEAQKWPPFEAVEAELAAWLAAVCIDVQWPQKPS
                     ATIVSLCWLASRWVCLYIPPFLLEAYRHETMASTLDEASLLALFGAAPTEVALTETQL
                     RKLCRLRSHCPAGKTELQWLLKRIGRVCRPDDDIGGRQRRANLVRRMAPRVFNAFALS
                     PAAAMAYCWLLLECDLTYDRDGNALAPSTYYEYLSRVRWVLEAHWPEGRRASELSAEE
                     WLQLTVAMRQVRDGESAENVELRLIAWRRIVSTLAGNADHRGASAALIDMGGAVSVTT
                     YRPSAASALVLEAQLPRARDHAEALFGDQSLERLQALAQLALLVEGANRRGELCATKP
                     SDVALDGTFVAFIGNGLEPGKTWWARRITFVSADSAELLVELRRYCQNLSVPPKFLFA
                     ESEGVVSKRLALERYRQLVDLLRAVYGNQAIEGHSCRGSAAMRMLAGNWETLFRQFVN
                     GPLELKHAQALLQSLIGDGPAHLAEVLTRIGHASHKSFVKHYCSAWPLWYAAAMRATQ
                     KTLRLRSSLIRHMPAIAEAKTKADEKAAHIEKALDAHRAFIYATPANQPKDSWAWALM
                     RAHWPGWKR"
     gene            4541..5791
                     /locus_tag="PKV56_15800"
     CDS             4541..5791
                     /locus_tag="PKV56_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69323.1"
                     /translation="MNPRPVVQGAAETPLTKRWLLLRDLLNEQQRRRWAAIEADSIGD
                     GGIQTVVRATGMARQTIQRGLEELNVHRDEGSLGPWFLDPTLAQREEGGGRKSRAGDL
                     LSALATRMNQQAAGKNSREVVFRWTCSSLRELAKGPPAVASHTHVALLLAQAGYDIVT
                     EPWRARSQHAEYVPRQYSSVASAVTTALDAGQPVVVLSLSKARRRDDLLCRRERDEMR
                     RVARADRALATTIWGRDCWDEGVLGETLARQGWSSSAPDVQTVMLAAQAVRQWWDAGG
                     QRKHGGCGEVLIIANALGLDSELEARWRLELSRFAVEAGVGVRSCYLPGGICKWTHIA
                     SRLGAVLAIDDEGDFVGRHKVTCRVIESASRHSHPETRRGRRGARPVAIDAGQAWPRF
                     EIRYNKKDEPLPMKNWDYSLSIMK"
     gene            complement(5876..6148)
                     /locus_tag="PKV56_15805"
     CDS             complement(5876..6148)
                     /locus_tag="PKV56_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69324.1"
                     /translation="MKTYGTMLFPAACDGRPWGKVSQYGSAKAVLAAAGVPDSEGGSF
                     KLRHTFALRQLRRGTPPERVAQWLGLSDVAALTPYTRLLIAPVEVV"
     gene            complement(6283..6825)
                     /locus_tag="PKV56_15810"
                     /pseudo
     CDS             complement(6283..6825)
                     /locus_tag="PKV56_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6896..>7282
                     /locus_tag="PKV56_15815"
     CDS             6896..>7282
                     /locus_tag="PKV56_15815"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX69325.1"
                     /translation="MPGTLAHQGLERIAKIFKIEAEIRGKSALRRRRVRQLQTRPVLD
                     DLKAWMNETLAQVSAKSPMALAIGYAVSNWSALVRFVGDGRIEAENNAAERALRAVAI
                     GRKNFLHLGSDAGGNSAAVIYTLIGTA"
BASE COUNT         1267 a         2311 c         2477 g         1227 t
ORIGIN      
        1 tcgcgcttcc agccgggcca gtgcgcgcgc atcagcgccc aggcccatga gtccttgggc
       61 tgattggctg gcgtggcata gatgaaggcc cggtgcgcgt ctagcgcctt ctcgatgtgt
      121 gccgccttct cgtcggcctt ggtcttcgcc tcggcaatgg cgggcatgtg cctgatcagg
      181 gacgatctga gtcgcagggt cttctgcgtc gcgcgcatgg ctgcggcgta ccacagcggc
      241 caggcgctgc aatagtgctt gacgaagctc ttgtgcgatg cgtgtccgat gcgtgttagg
      301 acctcagcca ggtgcgcagg cccatcgccg atcagcgact gcagcagcgc ttgcgcgtgc
      361 ttcagttcca gcggcccgtt gacgaactga cggaaaagcg tctcccagtt gccggccagc
      421 atgcgcatgg ccgcggaccc tcggcagctg tggccctcga tggcctggtt gccgtacacg
      481 gcgcgtagca ggtcgacgag ctgccgatag cgttcaagtg cgagcctctt gctgaccacg
      541 ccttcgctct ccgcgaagag gaacttcggc ggtaccgaca ggttctggca atagcgccgc
      601 agctcgacca ggagttcggc agagtcggca gagacaaacg tgatgcggcg cgcccaccag
      661 gtcttgccgg gctcaagtcc attgccgatg aacgcgacga aagtgccgtc cagcgccaca
      721 tcgctgggct tcgtcgcgca cagctcgcca cgacggttgg caccctccac cagcagcgcc
      781 aattgcgcga gtgcctgcag gcgttccagc gactgatcgc cgaacagggc ctccgcatga
      841 tcacgcgccc gcggcagctg tgcttccagc accagcgcgc tggccgcaga gggccggtag
      901 gtggtgacgc tgaccgcgcc acccatgtca atcagcgcag cggacgcgcc gcggtggtcg
      961 gcgttgccag ccagagtgga gacgatgcgt cgccaggcga tgagacggag ctcgacgttc
     1021 tcggcggact cgccatcccg cacctggcgc atggcgacag tgagctgcag ccattcctct
     1081 gcgctcagtt cgctggcgcg cctgccttct ggccagtgag cctcaagcac ccagcgcaca
     1141 cgcgacaggt actcgtagta ggtgctgggc gccagggcgt tgccgtcacg gtcataggtg
     1201 aggtcacact cgagcaacag ccagcaatag gccatcgcgg cggccggcga caaggcgaac
     1261 gcgttgaaaa cccggggtgc catgcgccgg acgaggtttg cacgtcgctg gcgcccgccg
     1321 atgtcgtcat cgggccggca cacccggccg atgcgcttca atagccattg cagttcggtc
     1381 tttccagcgg ggcaatggct gcggagtcgg cacagcttac gcagctgcgt ttcggtcaac
     1441 gcgacctcgg tcggtgcggc tccgaacaat gccagcagcg aggcttcgtc cagcgttgag
     1501 gccatcgtct cgtggcgata tgcctccagc aggaagggcg ggatgtacaa gcacacccag
     1561 cgactggcca gccagcacaa tgacacgatg gttgcgctcg gcttctgcgg ccattgaacg
     1621 tctatgcaga ctgccgccag ccaagccgcg agctcggcct cgaccgcctc gaacgggggc
     1681 cacttctggg cttcatcacg cgcaaggcgt gtcggcggct gcaccggcat gcagatcggc
     1741 tggccgctgt cgcgcgtgaa ccggagcatg gctgtgcgac cggcctcgag cgggtcaact
     1801 ggcacggcgc gcaggcattc ccaggctgca cgaacatcgg cgctgtgcac aaggccgtcg
     1861 acgaccacaa gcgacaggag ggcgcgggcc cagggcgaca acagctcgga cgtgccggtg
     1921 tgttcgcgca gagcgtcgat gacgcgaatg gccggcagcg tatggctgtc gcagtggtgg
     1981 cgcggctggt cgctggcacg gctgttcaga ttgcggcggc ggtcgatgtg gcgcaggtcg
     2041 acgcccacag cgacctctgg atactcgccg gggatggcgt caccgaaagc gccaagggac
     2101 aggcgggaga gcaccgtgtc gcacagctct cgcgttccct caaggaggca gtggatgggc
     2161 agcgacaggc aaggggagcc cacgtgggca aggccccgac cgtggccggt gatgaccaac
     2221 tccgcccacc actgggggct gcatgccgcc tgggtgaccc aaaacctgcg ccccatgttt
     2281 tgcggcccgc gccagagctg cggcacgagt gccattgcat cggcgtccag tggtccggtt
     2341 gccaggattt gccttccgcg gcggtccatc gttcgcagtc gcaagaacag catcgacgac
     2401 ggccgcgggt cgataagcaa gaccgggatt gctgcatcct cgacgcccag cgcgcgcagg
     2461 cggtcgcgtg cggcgatacg aagcgcccag acaacccgta tgacttcccg cactgcgggc
     2521 gtgaggggaa tcaggcgttc gctactgggc ttggttcgct tgtcatccag gcgaagccac
     2581 tggtcacttg cgcagacgtc gcgcagcgtg ggttgcgttc gctgctgggc ccgtccaccg
     2641 gtcagcagct tgaacgcccg gcgtcctgcg gccatgccct tgttcagcgc attgacgacg
     2701 gcatcttgcg tcgaggcgat gccgatctcg ccaaaggctg cattggcgtt cgagcagtag
     2761 tggcgccact cggcccggac ttcttcgtca tctggcgcgg ccggcagatc gtccggctcg
     2821 gggtagacga tcgggccagg ggtgcccagg cccaggaact ggaagacgcg tgacgccagt
     2881 tcgtgttgcc gagcgggatt cggctggtaa tagttggccg ccgaagccgg ggccaacgaa
     2941 gtgttcagac tctggagccc cgcctccagc ggacttgcgc cacaagccag ataaaccagg
     3001 cccagcgagt gcgccatgcg cgccgaccag gccggatgag ccgggtcgct caacgacttg
     3061 atccaggcct ctgcctggct caccagatcg tatagcgtgg ctgggtcgaa cagatccttg
     3121 aaccgccgtg cctgcggctt ggcagtccac aggctttcga tggcgtctgc cgcggccgat
     3181 ggggtgtaaa gcggatacca cgtcggacct ggctggttgt catcgagctt cagcagcagg
     3241 cgtcggtgcg ccaacacgca ccgcttctgg cgggagtacc agagatcgcc gttgttctcc
     3301 aggctcagct ccagcgtcgg gttgaagtcg tgcgagatca cgagcgccat cacgccgagc
     3361 agcgaggcct cgaagttggt cgccaccgtt tcgccaacaa gggtcagctg ggcggccgcc
     3421 gtgaccgcct tcaagcgttg cggcggctgc cggttccacg ccgactgaat tgcgaagagc
     3481 tcggccatgg tcgcgaacga cgcggccttg ggcagacttc gtaccgacgg aggcagacag
     3541 gaggcgcgcg gggtcacatc cgcacggggc tgcgaagggc ccggcgccaa taccatcgcc
     3601 atgaccgtgc ccgcttcaga caacagccca tcttggcgcc cgtcgtcacc ttccacgtca
     3661 tcgaccaggg tgtcggtacc cgggtcgggc accttgcgca ggctcgtctg cgcggcgtgc
     3721 aagtggctgg caagggcgcg cgcgtcgtcg agctgctttc ccgcggcgaa ctcggcaagc
     3781 ctgtcgaggg tctcctggtt cagcggcgag ctgacaagtt gggccaacaa ggtccaggcc
     3841 tcgcgcgacg cgcgccgaac ctcggtgccg tgcgcgtagg ccatgctgga cacgcgcggc
     3901 agcagcgctt cgggcagacc ttcctttgcg cgaatggcct cgagcagtgc caggctcagc
     3961 gagacgcaga ctccgacctc cagcgcatcg aaccttgcct tggacaggcg cgcgagcgcg
     4021 gaccaactgc gctcgccttc ctgggcgttg gcatcgagca aaagcctcgg gaagtccgtt
     4081 gccagccgcg cgttgaaggc catcgagctg gcaaggtcag cccagttggc cagcatgtcc
     4141 agctccgcat gcgtggcgtg aacttcgaag acatgcgcgg ctacctggag cgcgatcgcg
     4201 gctccctcgc cgttcggacg caagccaagg gccttggcaa ggcgcctgcc gaagggcaac
     4261 ttcagcgggt cgtcaacgat gcgccgcagc caatcgggcg cgcctcggtc aaaagcccgg
     4321 acgtcactcc gcgccacaaa ggctcctcgt ccagcccgga cggaggttgt gttcagtggc
     4381 ggtgttgcct gggccctgcc tgcatgaatc agggcgctgt ggggtggaat gtagggtgtc
     4441 gtccatctcg gagtccaagg aggccgaccc agaagatatg acccgaaacg cctgactttc
     4501 ccagcgcaat gcggtgcgtt agaaattaga attgcacacg atgaacccaa ggccagtagt
     4561 acaaggggca gccgagacgc cgctgaccaa gcggtggctg ttgcttagag acctgctgaa
     4621 cgagcagcaa cggcggcgct gggcggcgat cgaagccgat tcgattggcg atggaggcat
     4681 ccagactgtc gtccgggcca ccggcatggc tagacagaca attcagcgcg gcttggaaga
     4741 gctgaatgtc catcgcgatg agggatcgct gggcccgtgg ttcctggatc cgacgctggc
     4801 tcagcgagaa gagggaggtg ggcgcaagag cagggccgga gaccttctgt cagcgctcgc
     4861 aacgcggatg aatcaacagg ccgctggcaa gaacagccgg gaggtcgttt tccgatggac
     4921 ttgttcgagc ttgcgcgagc tggccaaggg cccgcccgcc gtggccagcc acactcacgt
     4981 tgcgttgctg ctcgcgcagg ctggctatga catcgtcacg gagccctggc gggcgcgaag
     5041 tcagcacgcc gagtacgtgc cgaggcagta cagcagcgtt gcctcagccg tgacaacggc
     5101 actggatgcg ggccagccag tcgtcgtact gagtttgtcc aaggcacgcc gcagggacga
     5161 cctgctctgt cgcagggaac gcgatgagat gaggagggtc gcacgcgccg atcgtgccct
     5221 tgccacgacg atctgggggc gcgactgctg ggacgagggc gtgctgggcg aaacgctggc
     5281 gcgccaggga tggtcttcct cagcgcctga cgttcagacc gtgatgctcg ccgcccaggc
     5341 ggtccgtcag tggtgggacg ccggagggca gcgcaagcat ggggggtgtg gggaagtgct
     5401 gattatcgcg aacgcgcttg gactcgatag cgagctcgaa gcgcgctgga ggctagaact
     5461 ctcgcgcttt gcggttgaag cgggcgttgg tgtccgctct tgctacttgc caggcggcat
     5521 ctgcaagtgg acgcacatcg ccagtcgcct gggcgcagtg ctggccatcg acgatgaagg
     5581 tgatttcgtg gggcgacaca aagtaacttg ccgggtcatc gagtctgcct cgaggcactc
     5641 gcacccagag actcgaaggg ggcggcgcgg tgcccgacct gtcgccattg acgccggcca
     5701 ggcgtggccg cggtttgaga tccgttacaa caagaaggat gagccgctgc cgatgaagaa
     5761 ctgggactac tcgctctcca tcatgaaata gtaatatctt acgaaatagg cgaagtgacg
     5821 ccctcacaga aacttcgcgc cgttcttttc tgtgtttgcg cgcgaaaagg gccggtcaca
     5881 ccacttcgac aggcgcgatc aacagccgcg tgtacggagt cagcgcggcg acatcgctca
     5941 agcccagcca ctgcgccacc cgctcgggcg gggtcccgcg tctcagctga cgcaacgcga
     6001 aggtatggcg cagcttgaag ctgccgccct ccgaatcagg cacccctgca gcggccagca
     6061 cggctttcgc cgagccgtac tgcgacacct tgccccaggg ccgtccatcg catgctgcgg
     6121 ggaacagcat cgttccgtac gttttcacgt cctccgcaag ctgcgatcag cgttgacccc
     6181 gcgcagagct gcaggcctgc ggcgcggagc ttgttgggtt tatccggagg tatgcgtccg
     6241 gccaagtcat cttgacgctg gccgccggca ggctgccgtt cttcaggagg caggggacca
     6301 gcggtgcggc agcaactcgt cgatgcggtt ggccgggtgc gttggcagcc gttcgaggac
     6361 gtccttgaag tacgcgtagg cgtcgtggcc gttgatgcgc gccgagtgca gcaggctcat
     6421 gatggccgct gcgcgcttgc ccgcgcgcag gctgccggcg aacagccaat tcgcccgccc
     6481 gagggcaatc ggcctgatgt ggttctccac ccagttgttg gagatcggca cgtcaccgtc
     6541 gttgacgaag cgcgtgagtt cggcccagcg cttgaggctg tagtcgatgg ccttgatcgt
     6601 ggccgagcca ggtggcacca actgccgctg cgccagcagc cagcgatgga agacggccag
     6661 cacccgcctt gacttgcgtt gccgtacctg ccttcgcgca tcgctgggca gttcctcgat
     6721 ctcgcgctcg atccggaaca ggcactggtg gtaccgcagc gccttgcggc ccacctcgct
     6781 gcggtggttg gcccacaggt cgttgaactt ccttcgggcg tggctcttat ccagagctct
     6841 ggataagagc catgccaggc gcaagctgtg ggacatccac gagcgccagc acaagttgcc
     6901 cggcacgctg gcgcatcagg ggctggagcg gatcgccaag atcttcaaga tcgaagccga
     6961 gatccgcggc aagtcggcct tgcggcgaag gcgggtgcga caactgcaga cgcggcccgt
     7021 actggacgac ttgaaggcct ggatgaacga gaccttggcg caggtctcgg cgaagtcgcc
     7081 gatggcgttg gccatcggct atgcggtgag caactggagc gccctggtgc gcttcgtcgg
     7141 cgacgggcgc atcgaggcag agaacaacgc cgctgagcgc gcgctgagag ccgtggccat
     7201 cggtcgcaag aacttcctgc acctgggctc ggacgccggt ggcaacagcg ccgccgtcat
     7261 ctacacgctg atcggcacgg cc
//
LOCUS       DAOORV010000166         7255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12641_length_7255_cov_10.867639, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000166 DAOORV010000000
VERSION     DAOORV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12641_length_7255_cov_10.867639"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="aceA"
                     /locus_tag="PKV56_15820"
     CDS             <1..928
                     /gene="aceA"
                     /locus_tag="PKV56_15820"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOX69326.1"
                     /translation="NATFKRADEIQWSEGKNDVDYFAPIVADAEAGFGGVLNAFELMK
                     AMIEAGAAGVHFEDQLAAAKKCGHMGGKVLVSTRDAVSKLVAARLAADVMGTPTILLA
                     RTDAEAADLVTSDVDDNDKPFFTGERTVEGFYRSRNGIEQAISRGLAYAEYADLIWCE
                     TGTPDLAFAKKFADAIHAKFPGKLLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKF
                     QFITLAGFHSLNYSMFNLAYGYARQNMSAFVELQEAEFKAAEKGFTAVKHQREVGTGY
                     FDAVTTTIEALASTGALKGSTEDEQFFDKNDH"
     gene            1197..1988
                     /locus_tag="PKV56_15825"
     CDS             1197..1988
                     /locus_tag="PKV56_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOX69327.1"
                     /translation="MLTVLGIMPAHGQSPAGVHIEDMTWTEVRDALKSGKTTVIIPVG
                     GTEQSGPHMALGKHNVRVKVLAERIATSLGNALVAPVVAYVPEGQVFPPTQHMRFAGT
                     VTIPDDAFKAVIDGAARSFRQHGFTDVVLIGDHGGYQSLLEKVASRLNRDWAGSKARA
                     HFIGAYYEAAQGPYSRVLRDRGLNDAQIGTHAGAADTSLMLATDPGLVRRDELKSSGR
                     DAAAAKALGVSGEPGLSSAALGQLGVDLIVSRTTSAIRQAVALRH"
     gene            2182..3339
                     /locus_tag="PKV56_15830"
     CDS             2182..3339
                     /locus_tag="PKV56_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOX69328.1"
                     /translation="MPPVIDRHNLYSETGAGHLSPAVAGALERVYVPNRVANSVSVID
                     PKTLKVIDTFQVGKNPQHVVPSWDLRTLWVANNAERRTNGSLTPIDPLTGKPGASIAV
                     DDPYNMYWTPDGQHAIVVAEAHKRLDFRDPRSMALQYSIDTPDCGGINHADFSIDGRY
                     ALFTCEFNGSITKIDLVDKRVVGTLKLSRYYARPEVLSLLASPGRKPRKIPDPSQLGG
                     EICTTRGMPQDVRASPDGSLFYIADMMADGVHVVDGASLTQVGFIPTGVGAHGLYPSR
                     DGKLLYVANRGSNSVLGKRRGQGSVSVLDFASQKVLRTWPVPGGGSPDMGNVSADGRH
                     LWLSGRFDNVVYRFDTDNGAVDVIKVGQEPHGLTVWPQPGRYSLGHTGNLR"
     gene            complement(3344..4252)
                     /locus_tag="PKV56_15835"
     CDS             complement(3344..4252)
                     /locus_tag="PKV56_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOX69329.1"
                     /translation="MCLIALAIDQSRRFPLVVAANRDEFFARPAARLGWWEHAADGPA
                     ILAGRDLTAGGTWLGLTAEGRLAMVTNVRDPARMDPTAPSRGAIVPQWLSGREPTDRF
                     WMRTALSGYNGFNLIAADFKRGECFWASNLGAHPKRLERGVYGVSNASLDTAWPKTIA
                     LKARLRQAISVADSVDALSSALFSALADRQIAADTDLPSTGIPLELERQLSSVFIRAT
                     ERQYGTRCSTLVISERVGRGLVTRVMERSYTATGAVSLLRQATLKGWPPRYDDATDPA
                     PVEQAVVSTAPRRRVRSLLKPAQAQR"
     gene            complement(4243..4548)
                     /locus_tag="PKV56_15840"
     CDS             complement(4243..4548)
                     /locus_tag="PKV56_15840"
                     /inference="COORDINATES: protein motif:HMM:NF027614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HOX69330.1"
                     /translation="MPRELYVYFRADATRADAVRTAVTAMQAQLRCEFPGLQARLLRR
                     PDSSGGQHTWMETYAMLQDAHLDGVSELMTSTIEQRALPWRDLLDGARHIEAFDACA"
     gene            complement(4559..5485)
                     /locus_tag="PKV56_15845"
     CDS             complement(4559..5485)
                     /locus_tag="PKV56_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOX69331.1"
                     /translation="MTEPLNRSLAVPLTHWGVIRAHGADAAKFLHGQLTQDVLQLGRS
                     QARIAGYCSAKGRLLASFVLWKTSDDEVLLACHTSVLAATLKRLSMFVMRAQCKLTDA
                     TDEISLQGLTGDRAAGETEGLPAWGKRDDAEGAWIRLPDVDGLARAWRVTSAASALAA
                     DPVALRAWQQLEIDSGIVTIEAATVDQFVPQMLNFELIGAVNFQKGCYPGQEVVARSQ
                     YRGTAKRRTFLFSCDEAIAAGQEVFHSADPEQPAGMVAGAVQSVDARSYRALVEVKLA
                     ALACGSLHLGAVDGALLTPRPLPYEVPVAEAA"
     gene            5581..6576
                     /gene="mltG"
                     /locus_tag="PKV56_15850"
     CDS             5581..6576
                     /gene="mltG"
                     /locus_tag="PKV56_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOX69332.1"
                     /translation="MRRWFVGLLVALCLLAGLSAAALHAWLDRPLTLGTDTVEVSIEP
                     GTSPRSIADAWVRAGVQAPPWLLYEWFRWSGDARRIRAGSYEITRAATPRSLLNMMVR
                     GDETMAFVRLGEGWTLRQFRAELARADALRPTTAAMTEAELMAALDDASTPAEGRFYP
                     DTYSYSKGSTDLSVLRRAHLAMRQRLAAAWADRSPDTPLQSPDEALALASIIEKETGQ
                     PADRGKVAAVFVNRLRIGMPLQTDPTVIYGLGASFDGNLRKRDLQNDTPYNTYLRNGL
                     PPTPISMPSRASLLAAVRPEPIRALYFVARGDGSSEFSNDLAAHNRAVNKYQRGR"
     gene            6617..7246
                     /gene="tmk"
                     /locus_tag="PKV56_15855"
     CDS             6617..7246
                     /gene="tmk"
                     /locus_tag="PKV56_15855"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOX69333.1"
                     /translation="MRAEGAVRGRFITFEGIDGAGKSTHIGPVAAKLRASGHTVVVTR
                     EPGGTPLAERLRELLLSAPMDALTEALLVFAARRDHVQQVIEPALARGDMVLCDRFTD
                     ATFAYQGAGRDFDIAVLERLESWVHGDLQPDLTFWFDVSAEQAAERLAAARAPDRFEQ
                     LDTAFFRRVREGYRVRADAFGERFVRIDGSADRDAVGCQVMSVLAERDW"
BASE COUNT         1255 a         2422 c         2381 g         1197 t
ORIGIN      
        1 caacgccacc ttcaagcgcg ccgacgagat ccagtggagc gaaggcaaga acgacgtcga
       61 ctacttcgcg ccgatcgtgg ccgatgccga agccggtttc ggcggcgtgc tgaatgcctt
      121 cgagctgatg aaggccatga tcgaagccgg cgccgccggc gtgcacttcg aagaccagct
      181 ggccgccgcc aagaagtgcg gccacatggg cggcaaggtg ctggtgagca cccgtgacgc
      241 ggtgagcaag ctggtggccg cccgcctggc cgccgacgtg atgggcacgc ccaccatcct
      301 gctggcccgc accgacgccg aagccgccga cctggtgacc agcgacgtgg acgacaacga
      361 caagccgttc ttcaccggcg agcgcaccgt cgaaggcttc taccgcagcc gcaacggcat
      421 cgagcaggcc atcagccgcg gcctggccta tgccgagtac gccgacctga tctggtgcga
      481 gaccggcacg ccggacctgg ccttcgccaa gaagttcgcc gacgccatcc acgccaagtt
      541 cccgggcaag ctgctggcct acaactgcag ccccagcttc aactggaaga agaacctgga
      601 cgacgccacc atcgccaagt tccagcgcga gctgggcgcg atgggctaca agttccagtt
      661 catcacgctg gccggcttcc acagcctgaa ctactcgatg ttcaacctgg cctacggcta
      721 cgcccgtcag aacatgagcg ccttcgtgga gctgcaggaa gccgagttca aggctgccga
      781 gaagggcttc accgccgtca agcaccagcg cgaagtgggc acgggttact tcgatgccgt
      841 cacgacgacg atcgaggcgc tggcatcgac cggcgcgctc aagggttcga ccgaagacga
      901 acagttcttc gacaagaacg atcactgatc gcggcttcaa tgcaccgacc gccgcagcgg
      961 ttggtgcagt gatcagcccc gggcccgaag gtgtcacgca ccttcgggcc ttcttttttg
     1021 ggtcctgcgt gttcggtgtc agctgtctgg tcatgccaga ccgttaacgt ggacagcaca
     1081 cccgcagatc gcgggtggcg gccatgcgaa cgcgagtctc catcaccttg aatccggaac
     1141 ttccaagcgg catgccggca cgatcctgga cccgtcggtg gctttgcgtg ctgaccgtgt
     1201 tgactgtgct cggcatcatg ccggctcacg gccagtcccc agcgggtgtt catatcgagg
     1261 acatgacctg gaccgaagtg cgcgatgcgc tgaagtccgg caagaccacg gtgatcatcc
     1321 ccgtcggcgg gaccgagcag agcggtccgc acatggcgct gggcaagcac aacgtgcggg
     1381 tcaaggtgct ggccgagcgg atcgccacat cgctgggcaa cgcgctggtc gcgccggtgg
     1441 tcgcttacgt gccggaaggc caggtgtttc cgcccaccca gcacatgcgc ttcgcaggca
     1501 ccgtgacgat ccccgacgat gcattcaagg ccgtgatcga tggggccgcg cgcagcttcc
     1561 ggcaacacgg gttcaccgac gtggtcctga tcggggatca tggcggatac cagtcgctgc
     1621 tcgagaaggt ggcctcgcgc ctgaatcggg actgggccgg ttcgaaggcg cgcgcgcatt
     1681 tcatcggcgc gtattacgaa gccgctcagg gaccctacag ccgggtgctg cgggaccgcg
     1741 gattgaatga cgcccagatc ggaacccacg cgggcgcggc cgatacatcg ctgatgcttg
     1801 cgaccgaccc cggcctggta cgccgggatg aactgaagtc ttccggacgt gatgccgctg
     1861 cggcgaaggc gctcggcgtc agcggcgagc ccggcctgtc cagcgcggcc ctcggtcagc
     1921 tcggcgtgga cctcatcgtt tcccgaacga cctctgccat ccgtcaggcg gtcgctctgc
     1981 gtcactgatc ttcattgctc acttcattcc ttccccatct tgaattcacg caccctcttt
     2041 cgtcatccct tgctgcttcg tcgcgtcctg ccggtgctgt gcgcaattgc tgcaggcttc
     2101 gtgggcgcca acctgcgctc gcagacacct gcgaaatctc cggttgcatc gagcccgaca
     2161 gcatggcctt cggtgcccgg catgccacca gtgatcgatc gtcacaatct ttacagcgag
     2221 accggtgcgg ggcacctgag cccggcggtg gccggcgcgc tggagcgcgt ctacgtgccg
     2281 aaccgggtgg ccaattcggt gtcggtcatc gacccgaaga cgctgaaggt catcgataca
     2341 ttccaggtcg ggaagaaccc gcagcatgtg gtgccatcgt gggatctgcg cacgctctgg
     2401 gtcgccaaca acgccgaaag gcgcaccaat ggcagcctca cgccgatcga tccgctgacg
     2461 ggaaagccgg gcgcctccat cgcggtggac gacccctaca acatgtactg gacgcctgac
     2521 ggccagcacg cgatcgtggt ggccgaggcc cacaagcggc tcgatttccg tgacccgcgc
     2581 agcatggcat tgcagtactc gatcgacacc cccgactgcg gcggcatcaa ccatgccgac
     2641 ttctcgatcg atggtcgtta cgcgcttttc acctgcgagt tcaacggctc gatcacgaag
     2701 atcgacctgg tcgacaagcg cgtggtcggc acgctgaaat tgagccggta ctacgcgcgc
     2761 ccggaggtcc tttctctgct ggccagcccg ggcaggaagc cccgcaagat tcccgatccg
     2821 tcacagctcg gaggcgagat ctgcaccacg cgcggcatgc ctcaggacgt gcgggcgtca
     2881 ccggatggtt cgctgttcta catcgccgac atgatggccg acggggttca cgtggtcgat
     2941 ggcgcgagct tgacgcaggt gggtttcatt cccaccggag tgggcgcgca tgggttgtat
     3001 cccagccggg acgggaagct gctctatgtg gccaaccgtg gcagcaacag cgtgctgggc
     3061 aagcgccgcg gccagggcag cgtgtcggtc ctcgatttcg cgagccagaa ggtgcttcgg
     3121 acctggccgg tgccaggcgg aggcagcccg gacatgggca atgtcagtgc ggacggcaga
     3181 cacctctggc tctcgggccg attcgacaac gtcgtctacc gctttgacac ggacaacggt
     3241 gcggtcgacg tcatcaaggt gggccaggaa cctcacggcc tgactgtctg gccgcaaccc
     3301 ggccgctact cgctcggtca caccggcaac ctgcgctgaa ggctcagcgc tgcgcctgcg
     3361 cgggcttcag caggctgcgt acccgcctgc gtggtgcggt cgacaccacg gcctgctcga
     3421 cgggtgcggg gtccgtggcg tcgtcgtagc gcggtggcca gcctttcagc gtggcttgac
     3481 gcagcaggga aacggcgccg gtggcggtgt agctgcgctc catgacgcgc gtgaccagcc
     3541 cgcgtcccac gcgctcgctg atcaccagcg tcgagcaccg cgtgccgtac tgtcgttcgg
     3601 tggcgcggat gaataccgat gacaactgcc tctcgagctc cagcggaatg ccggtcgagg
     3661 gcaggtcggt gtcggctgcg atctggcgat ccgcgagtgc ggaaaacagc gcgctcgaca
     3721 gcgcatcgac tgaatcggcc accgagatgg cctggcgcag gcgggccttc agcgcgatgg
     3781 tcttgggcca ggccgtgtcc agcgacgcat tcgaaactcc atagacgcca cgctccagcc
     3841 gcttcggatg ggcgccgagg ttcgaggccc agaagcactc tccgcgcttg aaatcggcgg
     3901 cgatcaggtt gaatccgttg tagcccgaca gcgcggtgcg catccagaac cggtcggtcg
     3961 gttcacgtcc cgacagccac tgaggaacga tcgcaccgcg cgatggcgcg gtggggtcca
     4021 tgcgggcagg atcgcgcaca ttggtgacca tcgccaggcg gccctcggca gtcaggccga
     4081 gccacgtgcc gcctgcggtc aggtcgcgtc cggccaggat cgccgggccg tcggcagcgt
     4141 gctcccacca gcccagccgt gcggccgggc gtgcaaagaa ctcatcccgg ttggcggcaa
     4201 ccaccagcgg gaagcgccgg ctttgatcaa tggccagcgc gatcaggcac atgcatcgaa
     4261 cgcttcgatg tgcctggccc cgtccagcag atcgcgccag ggcaacgccc gctgctcgat
     4321 ggtcgatgtc atgagttcgc tcaccccgtc gaggtgggcg tcctggagca tcgcgtaggt
     4381 ttccatccag gtgtgctgcc cgccggacga atccgggcgc ctgagcagac gggcctgcag
     4441 gcccgggaac tcgcagcgca gttgagcctg catggccgtc acggccgttc gcaccgcatc
     4501 ggcgcgggtc gcatcggccc ggaaatagac atacaactca cggggcatga ggcgtcgatc
     4561 aggcagcttc ggcgactgga acctcgtagg gaagagggcg cggggtcagc aatgcaccat
     4621 cgaccgctcc cagatgcagg ctgccgcagg ccagggcggc gagcttgact tcgaccagtg
     4681 cccgatacga acgggcgtcg acggactgga cagcaccggc gaccatgcct gccggttgct
     4741 ccggatcggc gctgtgaaac acctcctggc cagcagcgat ggcctcgtcg caggagaaga
     4801 ggaacgtcct gcgcttggcg gtgccacggt actgcgagcg tgccaccacc tcctggcccg
     4861 ggtagcagcc cttctggaaa ttgactgcac cgatcaattc gaagttcagc atctgcggga
     4921 cgaactggtc gacggtggcc gcctcgatcg tcacgatgcc actgtcgatc tcgagttgct
     4981 gccaggcgcg caacgcgacc gggtccgcag ccaatgccga cgctgccgaa gtcacccgcc
     5041 aggcccgcgc caggccatcg acatcaggca gccgtatcca ggcgccctcc gcatcgtcgc
     5101 gcttgcccca tgccggcaag ccctcggtct caccagcggc ccggtctccg gtgagccctt
     5161 gcaacgatat ttcgtctgtt gcatcggtga gcttgcattg agcgcgcatc acgaacatgg
     5221 acagccgctt cagggtcgcg gccagcacgc tcgtgtgaca cgccagcagc acctcgtcat
     5281 cggacgtctt ccacaagaca aagctcgcca gcaggcgacc cttggccgaa cagtagccgg
     5341 cgatgcgcgc ctgcgagcgc ccgagttgca gcacgtcctg cgtcagctgg ccatggagga
     5401 acttggcggc atcggcaccg tgagcgcgta tcacgcccca gtgggtcagt ggcacagcga
     5461 gagaacggtt caggggctcg gtcatgcgcc gattatcatt tgcacgatgt tgccccgctg
     5521 ccttcggtgt catgcaagcg tcggcgtcaa accgggcgct gtgcagccgc gggacctcgc
     5581 ttgaggcgct ggttcgtcgg gctgctggtg gccctgtgcc tgctcgcggg cctgtccgcg
     5641 gctgcgcttc atgcatggct ggacagaccg ctgacactgg gcaccgacac cgtcgaggtg
     5701 tccatcgagc cgggaacctc gccccgcagc atcgccgatg cctgggtgag ggccggcgtg
     5761 caggcgcccc cgtggctgct gtatgaatgg ttccggtggt ctggcgacgc acgccgcatc
     5821 cgcgccggca gctacgagat cactcgcgcc gccacaccga gaagcctgct gaacatgatg
     5881 gtgcgcggag acgagacgat ggcgttcgtt cgcctgggcg agggctggac actgcgacag
     5941 ttccgtgccg agctggcccg cgccgacgcc ctcaggccga ccactgccgc gatgaccgag
     6001 gccgagctga tggcagcact ggacgacgcc tccacgccgg ccgaagggcg tttctacccg
     6061 gacacctatt cgtacagcaa gggcagcacc gatctctcgg tgctgcgtcg cgcacatcta
     6121 gccatgcgtc agcggctggc ggccgcctgg gccgaccgct cgcccgacac accactgcag
     6181 tcgcccgacg aggcgctggc gctggcatcc atcatcgaga aggaaaccgg gcagccagcc
     6241 gaccgcggca aggtggcggc ggttttcgtc aaccgcctgc gcatcgggat gccgctgcag
     6301 accgatccga ccgtcatcta cggactcggt gcatcgttcg acggcaacct gcgcaagcgc
     6361 gatctgcaga acgacacgcc ctacaacacc tacctccgca acggtctgcc gcccacgccg
     6421 atctcgatgc cgagtcgcgc ttcactgctg gcggcggttc gaccggaacc gatccgggcg
     6481 ctgtacttcg tggcgcgagg cgatggctcc agcgaattca gcaatgacct cgccgcccat
     6541 aatcgggccg tcaacaagta ccagcggggc cgttgacgac gaccggagca gggtcggatg
     6601 ccacgcaatg agacccatga gagcagaggg cgctgtgcgc ggtcgcttca tcacattcga
     6661 aggcatcgac ggtgccggca agagcaccca catcgggccg gtggcggcga agttgcgcgc
     6721 ctctggtcac accgtcgtcg tgacgcgcga gcccggcggc acgccgctcg ccgagcgcct
     6781 gcgggagttg ctgctgagcg caccgatgga tgccctcacc gaagccctgc tggtgttcgc
     6841 cgctcgacgt gaccacgtgc aacaggtgat cgagccggca ctggcgcgcg gcgacatggt
     6901 tctctgcgac cgattcaccg acgcgacctt tgcataccag ggcgccggcc gcgacttcga
     6961 catcgcggtc ctggaaagac tcgaatcctg ggttcatggc gacttgcagc cggatctcac
     7021 gttctggttc gatgtcagtg ccgagcaggc cgccgagcgc cttgcggcag cgcgagcccc
     7081 ggaccgtttc gaacagctgg acaccgcgtt ctttcgaagg gtgcgcgagg gatatcgcgt
     7141 ccgcgccgac gcatttggag agcgtttcgt gcgcatcgat gggtcggccg atcgcgatgc
     7201 ggtgggctgc caggtgatgt ccgtcctggc ggagcgtgac tggtgagcgg gccgg
//
LOCUS       DAOORV010000167         7187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12792_length_7187_cov_5.035053, whole genome shotgun sequence.
ACCESSION   DAOORV010000167 DAOORV010000000
VERSION     DAOORV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12792_length_7187_cov_5.035053"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="ilvC"
                     /locus_tag="PKV56_15860"
     CDS             complement(<1..775)
                     /gene="ilvC"
                     /locus_tag="PKV56_15860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOX69334.1"
                     /translation="MKVYYDKDADLSLVKGKNVTIIGYGSQGHAHAQNLSDSGVKVTV
                     GLRKGGASWSKAEKAGLKVAEVADAVKSADVVMMLLPDEQIGAVYKNDVEPFIKQGAS
                     LAFAHGFNVHYGQVIPRADLDVWMVAPKAPGHTVRSTYTQGGGVPHLIAVHADKSGKA
                     RDLALSYATANGGGKAGIIETNFREETETDLFGEQAVLCGGAVELIKAGFETLTEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGE"
     gene            complement(823..1314)
                     /gene="ilvN"
                     /locus_tag="PKV56_15865"
     CDS             complement(823..1314)
                     /gene="ilvN"
                     /locus_tag="PKV56_15865"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOX69335.1"
                     /translation="MKHIIALLLENEAGALSRVVGLFSARGYNIESLTVATTEDPSLS
                     RMTIVTNGSDEVIEQITKHLNRLIEVVKVVDLTEGAYTERELMLIKVRAVGKEREEMM
                     RMADIFRGRIIDVTEKSYTMELTGDSSKLDAFIEAIDRAAILETVRTGTSGIGRGERI
                     LRV"
     gene            complement(1469..3247)
                     /locus_tag="PKV56_15870"
     CDS             complement(1469..3247)
                     /locus_tag="PKV56_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HOX69336.1"
                     /translation="MDMSDADIKRAEPAPNSPPAHPVEPNGSEILVRCLHAENVKYLW
                     GYPGGAVLYIYDALYKQNVIEHVLVRHEQAAVHAADGYARATGEVGVALVTSGPGVTN
                     AITGIATAYMDSIPMVIITGQVPTPAIGLDAFQECDTVGITRPIVKHNFLVKDVRDLA
                     LTLKKAFHIARTGRPGPVVVDIPKDVSLKTAPFSYPSTLEMRSYSPVRKGHAGQIRKA
                     VGLLLAAKRPYIYSGGGVILGSASAELRELVNLLGFPCTNTLMGLGAYPSSDPKFLGM
                     LGMHGTYEANMTMQNCDVLLAVGARFDDRVIGNPAHFASVERKIVHIDIDPSSISKRV
                     KVDIPIVGDVKEVLQELIAQIKESQAKPDAGSLSSWWAQITEWRKRECLKYTNSDVVI
                     KPQYVVEKLWELTRHSDAYITSDVGQHQMWAAQYYRFDEPRRWINSGGLGTMGVGLPY
                     AMGIKLAKPEADVFCITGEGSIQMCIQELSTCKQYNTPVKVISLNNRYLGMVRQWQQL
                     DYGGRYSHSYMDALPDFVKLAEAYGHVGLKIEKPSDVEGALREAIRLKDRTVFLDVRT
                     DQEENVWPMVKAGKGISEMLLGSEDL"
     gene            3500..4066
                     /locus_tag="PKV56_15875"
     CDS             3500..4066
                     /locus_tag="PKV56_15875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX69337.1"
                     /translation="MASEQELSEFLKSVDKRAFKRAVYAVRDEDAALDIVQEAMIKLA
                     EKYADRPAAELPPLFQRILSNATMDWFRRQKVRGALFRNFSDFEPANGDGDFDILETL
                     EALAGTAGAESASEAVSREQILLLIESEIELLPTRQREAFLLRYWEELDVAETALAMG
                     CSEGSVKTHCSRAVRALAKGLKAKGVAP"
     gene            4063..4512
                     /locus_tag="PKV56_15880"
     CDS             4063..4512
                     /locus_tag="PKV56_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HOX69338.1"
                     /translation="MNVVKNLTPIRADALEARFGLRLAARLSEGADQLPHDLTERLRF
                     ARETALARGLSASSVRAKANTVGFSSSGTLSLGGDPSRGKPWWTSLTAIVPMVALVGG
                     LYLIGHFHAREQIAAAAEVDAALLADDLPPAAYSDPGFVEFLKTARD"
     gene            4614..5336
                     /locus_tag="PKV56_15885"
     CDS             4614..5336
                     /locus_tag="PKV56_15885"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HOX69339.1"
                     /translation="MAAPAVPAQAGVQGSGLDAERGPNWKSLSASQQSALKPLERDWR
                     GIGADHKEKWLEIAAQFPAMSVDERKRVQARMSEWAAMTPQQRGAARFQFKQAQQLAP
                     SNRQARWEAYQALPAEEKKQLASRAEPPASTGSALRRSRSTPNDGKASSGVQPKSNVV
                     AAPDVGRPVRSVAPTLVQAPTGATTTLISKRPAPPVHEQAGLPKITASPNFVNPSTLL
                     PRRGPQGAAVIAASASAPPARP"
     gene            5344..5883
                     /locus_tag="PKV56_15890"
     CDS             5344..5883
                     /locus_tag="PKV56_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOX69340.1"
                     /translation="MTSQVVSSAPVAVRAPSLRRRMACFVYEGLMLFGIGLLPGAIGA
                     LFLALTGHAHPLQSDAALRVIAFVIYGIYFTWFWSRRGQTLPMQTWHIRVVTIDGQPL
                     SQTRALLRYLVSCIWFAPAAVLASLNHWARWDALAAIGVGVIAYALLALLQPQRQFWH
                     DVVCGTQLVDAPLLPRHKR"
     gene            5933..6316
                     /locus_tag="PKV56_15895"
     CDS             5933..6316
                     /locus_tag="PKV56_15895"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOX69341.1"
                     /translation="MTTNQYKGRTGLDRVLKATGYSLSGLRIAYRGESAFRQEVWLAV
                     VLLPAAFWLGTTWIEVAALAGTVMLVLIVELLNSGIEAAIDRVSFELHDLSKRAKDLA
                     SAAVFLSLMLCLGVWCAAGWHRFVI"
     gene            complement(6370..6708)
                     /locus_tag="PKV56_15900"
     CDS             complement(6370..6708)
                     /locus_tag="PKV56_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOX69342.1"
                     /translation="MKQITAILKPFKLEEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVMIKDTDLDRCIEAIVKAAKTGKIGDGKIFVTSIEQVVRI
                     RTGETGESAI"
     gene            complement(6741..>7187)
                     /gene="nadE"
                     /locus_tag="PKV56_15905"
     CDS             complement(6741..>7187)
                     /gene="nadE"
                     /locus_tag="PKV56_15905"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOX69343.1"
                     /translation="NKSEMATGYCTLYGDMAGGFAVIKDVAKTLVYRLCAWKNLQAGD
                     GRGEVIPNRILTRPPSAELRPDQKDQDSLPEYAVLDEVVERYMENDQSIDEIVAAGFA
                     RSDVERVTRLIKINEYKRRQAPVGIRITHRAFGRDWRYPITSKFRA"
BASE COUNT         1190 a         2187 c         2293 g         1517 t
ORIGIN      
        1 actcgccgta ttcggcgttg ttcgagatcg agtagttcat gttggcgatg ccgccctcat
       61 agatcaggtc gacgatcagc ttgagttcgt gcaggcactc gaagtacgcc atttccgggg
      121 cgtagccagc ttcggtcagc gtttcgaatc cggccttgat cagttcgacc gcgccgccgc
      181 aaagcacggc ttgctcgccg aacaggtcgg tctcggtttc ttcacggaaa ttggtctcga
      241 tgatgccggc cttgccgcca ccgttggccg tggcgtagct cagtgccagg tcacgcgcct
      301 tgccgctctt gtcggcatgg actgcgatca ggtgcggcac accgccacct tgggtgtagg
      361 tcgaacgcac ggtgtggccg ggagccttgg gcgcaaccat ccagacgtcg aggtcagcgc
      421 gaggaatcac ctgcccgtag tggacgttga atccgtgtgc aaatgccagc gacgcaccct
      481 gcttgatgaa gggctcgacg tcgttcttgt agacggcgcc gatctgctcg tcgggcagca
      541 gcatcatcac cacgtcagca gatttgacgg catcggcgac ttcggcgacc ttcaggccgg
      601 ccttctcggc cttcgaccac gatgcgccgc ccttgcgcag tccgaccgtg accttgacgc
      661 cgctgtcgct caggttctgc gcgtgagcat gaccttgcga gccgtagccg atgatggtga
      721 cgttcttgcc cttgacgagg ctgaggtctg cgtctttgtc gtaataaacc ttcatgatga
      781 tctcctggat tcagttgttg tggttcggga tggatgcggg ggtcacacgc gcaggatgcg
      841 ctctccgcgc ccgatcccgc tggtaccggt gcgcacggtt tcaagaatgg cggcccggtc
      901 gatcgcttca atgaaagcat cgagcttcga tgagtcgccg gtcagttcca tggtgtagct
      961 cttctcggtg acgtcgatga tgcgtccgcg gaagatgtcc gccatgcgca tcatttcctc
     1021 gcgctccttg ccgacggctc tgaccttgat cagcatcagt tcgcgttcgg tgtaggcgcc
     1081 ctcggtcagg tcgacgacct tgaccacctc gatcaggcgg ttcaggtgct tggtgatctg
     1141 ctcgatcact tcgtccgagc cgttggtgac gatggtcatt cgagacaggg aaggatcttc
     1201 ggtcgtggct acggtcaggc tctcgatgtt gtagccgcgg gccgagaaca ggcccaccac
     1261 cctggagagc gcgcccgcct cgttctcgag cagcaaggca atgatgtgtt tcatgtgcat
     1321 gtcggcccac cttcggctgc aaggcagcgg tagctgccag tactttggcc aggcctgaag
     1381 aagtttcgga ttgagtggaa tgtgaggcgc cgttcgtcga cgggtcggcc ggtgcggtaa
     1441 cgccggcgtc gggtcgacgt tcagaacatc acaggtcttc ggaccccaga agcatctcgc
     1501 tgatgccctt gccggccttg accatcggcc agacgttctc ttcctgatcg gtacggacgt
     1561 cgaggaacac ggtgcgatcc ttcaggcgga tggcctcgcg caaggcgcct tcgacatcgg
     1621 acggcttttc gatcttcagt ccgacgtggc cataggcctc ggccagtttc acgaaatcag
     1681 gcaaagcgtc catgtagctg tgcgaatagc ggccgccgta atcgagctgc tgccactgcc
     1741 ggaccatccc gaggtaacgg ttgttgagtg agatgacctt gaccggcgtg ttgtattgct
     1801 tgcaggtcga gagctcctgg atgcacatct ggatcgaacc ttcgcccgtg atgcagaaaa
     1861 cgtcggcctc gggcttggcg agcttgatgc ccatggcgta gggcagcccg acgcccatcg
     1921 tcccgagacc acccgaattg atccagcgac gaggttcgtc gaaacggtag tactgcgccg
     1981 cccacatctg gtgctggccg acatctgacg tgatgtaggc gtcgctgtgc ctggtcagct
     2041 cccagagctt ctcaaccacg tactgcggct tgatgaccac atcgctgttg gtgtacttca
     2101 ggcactcgcg cttgcgccac tcggtgatct gcgcccacca ggacgacagc gaacctgcgt
     2161 ccggcttggc ctgcgattcc ttgatctgcg cgatcaattc ctgcaggact tccttcacgt
     2221 cgcccacgat cgggatgtcg accttgacgc gcttggatat cgaagacgga tcgatgtcga
     2281 tgtggacgat ctttcgctcg acagaagcaa agtgcgccgg gttgccgatc acgcggtcgt
     2341 cgaaacgcgc gccgacggcc agcaacacgt cgcagttctg catcgtcatg ttggcttcgt
     2401 aagtcccgtg catcccgagc atgcccagga acttcggatc gctcgaaggg taagcgccca
     2461 gacccatcaa cgtgttggtg cagggaaatc cgagcagatt gaccagttcg cgcaactcgg
     2521 ccgatgcgct gccgaggatg actccgccgc cgctgtagat gtaggggcgc ttggctgcca
     2581 gcagcaggcc aaccgccttg cggatctgcc ccgcatgccc cttgcgcacg gggctgtacg
     2641 agcgcatctc cagtgtcgat ggataagaga acggcgccgt cttcagtgat acgtctttcg
     2701 gaatgtcgac cacgacagga ccgggacgac cggtgcgtgc aatgtggaac gccttcttca
     2761 gcgtcaaggc gagatcccgg acgtccttga ccaggaagtt gtgcttgacg atcgggcgcg
     2821 tgatgcccac ggtgtcgcat tcctggaatg cgtcgagccc gatggcgggg gttggcacct
     2881 gaccggtgat gatcaccatc gggatgctgt ccatgtaggc ggtcgcgatg ccggtgatcg
     2941 cgttggtgac gcccggaccg gacgtgacca gcgcgacgcc tacctcgccc gtcgcgcgag
     3001 cgtagccgtc tgcagcgtgc acagccgcct gctcgtggcg caccagcacg tgctcgatga
     3061 cgttctgctt gtacagcgcg tcatagatgt agagcaccgc tccgccgggg tatccccaga
     3121 ggtacttgac attttccgcg tgcaggcagc ggaccagaat ctcggagccg ttgggctcga
     3181 cagggtgggc cggtggcgag ttgggcgccg gttcggcgcg cttgatatca gcgtcagaca
     3241 tatccatgtt gaacctttgc gattttctct aacgaaaaac catcggtgcg cctctcctcg
     3301 ctcttgtgga gcgggtgtgg cgcctgcggg accacccggg aggtgcctca ggtcgagagc
     3361 accgggaacg gtccgtctgt tgtgcaaccc gcgattatgg cacctgaact gtgatggcgg
     3421 gtcagcctgc gatgcccaag ctggtgcagt gcgatactcg ctggctgcgt gactcgcccc
     3481 gtgatgaggc gcatttgctt tggcttccga acaagaactg tccgaatttc tgaaaagcgt
     3541 cgacaagcgc gccttcaagc gagccgtcta cgctgtgcgc gacgaggatg ccgcgctgga
     3601 tatcgtgcag gaggccatga tcaagctggc tgaaaagtac gctgaccgcc ccgcggccga
     3661 gttgcccccg ctgttccagc ggattctctc gaacgcgacg atggactggt ttcgccgcca
     3721 gaaggtgcgt ggtgcgcttt tccgtaattt ttcggacttt gagccggcca acggtgatgg
     3781 cgacttcgat attctggaga cgctggaagc gctcgcggga actgcgggag cagaaagtgc
     3841 ctccgaggcc gtttcccggg agcagatatt gctgttgatc gagtccgaga tcgaactctt
     3901 gcccacccgt caacgcgaag cgtttctctt gcgttactgg gaagagttgg atgtcgccga
     3961 gacagcgctt gccatgggtt gttcagaggg gagcgtcaag acgcattgct cgcgcgctgt
     4021 acgcgcacta gccaaggggc tgaaggccaa aggagttgct ccatgaatgt cgtgaaaaac
     4081 ctcacaccga ttcgggccga tgcgctcgaa gcgcgctttg gtctgcgact ggccgcccgt
     4141 ttgagcgaag gtgctgacca acttccgcat gacctgacgg agcgcctgcg ctttgcgcgc
     4201 gaaaccgctt tggctcgagg actttctgca tcgtcggtac gggccaaagc gaatacggtt
     4261 ggcttctcgt cctccggaac gctttccttg ggcggggatc cgtcgcgtgg caagccttgg
     4321 tggacgtcat tgacagcgat cgttccgatg gtggctttgg tgggtggtct gtacctgatc
     4381 ggtcatttcc atgctcgtga gcagattgca gcggccgctg aggtcgacgc agccctgctc
     4441 gcggacgacc tgccgcccgc tgcctacagc gacccgggct tcgtcgagtt cctcaagacg
     4501 gcccgggact gagttccaac ggcatgccgc tcgtcgccaa tccgatctgc cgccccattc
     4561 gcgttctggc gattggagtg gtagcggttg ctgcgtgcgt gtggggggag tcgatggcag
     4621 cgccagccgt tcccgcccag gcaggcgtgc agggttcggg cttggatgcc gagcgcggtc
     4681 cgaactggaa gtcgctttcg gcctcgcagc aatcggcgct gaagcctctg gagcgcgatt
     4741 ggcgcggcat cggtgcggat cacaaggaga aatggttgga gatcgccgcg cagttcccgg
     4801 ccatgtctgt ggacgagcgc aagcgtgttc aggcgcgaat gtccgagtgg gctgcgatga
     4861 cacctcagca gcgcggtgcg gcgcgcttcc agttcaagca ggcgcagcaa ttggctcctt
     4921 cgaatcgtca ggcgcgatgg gaggcctatc aagccttgcc tgcggaggaa aagaagcagc
     4981 ttgcctcgcg cgccgagcct cccgccagca cgggctcggc gctgcgtcgc tctcgctcga
     5041 caccgaacga tggcaaggcc tcgtccggcg tccagcccaa gtcgaacgtg gttgctgctc
     5101 cggatgtggg ccgaccagtt cgctcggtcg cacccacgct ggtgcaggca ccgactggcg
     5161 ccacgaccac gctgatttcc aagcgtccag cgccgccagt acatgagcag gccgggttgc
     5221 cgaagatcac ggcatccccc aatttcgtga acccgtcgac gctgttgcct cgacgcggcc
     5281 cgcagggcgc agcggtgatt gccgccagtg cctcggcgcc gcccgctcgc ccgtgatgcg
     5341 ggcatgacgt ctcaggtcgt gagttctgcg cctgttgccg tgcgggctcc cagcctgcgg
     5401 cgacgcatgg cctgcttcgt ctacgaaggc ttgatgttgt tcggcatcgg actccttccg
     5461 ggtgcgatcg gtgcgctttt cctcgccctg accgggcacg cgcatccgct gcagagcgat
     5521 gcagcgctgc gcgtcattgc gttcgtgatc tacggtatct atttcacgtg gttctggtcc
     5581 cggcgcgggc agactctgcc gatgcagacg tggcacatcc gcgtcgtgac gatcgatggc
     5641 cagcctttga gccagacccg ggccctgctg cgctacctgg tcagctgcat ctggtttgcg
     5701 ccggctgccg tgctcgcctc cctgaatcac tgggcacgct gggatgcact ggcagcgatc
     5761 ggtgtcggcg tgatcgctta tgccttgctg gccctgttgc agccgcagcg gcagttctgg
     5821 cacgacgtgg tttgcggcac ccaactcgtg gacgctccgc tgctgccgcg ccacaagcgc
     5881 tgagtcgcgc tcgcgcgagc cggcatgccg tgcgcgtgca aaatcttcgt caatgacaac
     5941 caatcagtac aagggtcgca ccggtcttga ccgtgtcttg aaggccaccg gctattcgct
     6001 gtccggcctg cggatcgcct accgtggcga gagcgcattc cgccaggagg tctggctggc
     6061 agtggtgctg ctgccagccg ctttctggtt gggtaccacg tggatcgagg tggctgcgct
     6121 tgccggaacg gtgatgctgg tgttgatcgt cgaacttctg aactcgggga tcgaggccgc
     6181 catcgaccgc gtgtcgttcg aactgcacga tctgtccaag cgcgccaagg acctggcgag
     6241 cgctgcagtg ttcctgtcat tgatgctgtg tctgggggtt tggtgcgccg cagggtggca
     6301 ccgtttcgtc atctgacagg cgaaaaaaag cccgcgtccg cgggctggag aacagcgcat
     6361 tcctgcgcct cagatggcag attcgccggt ctcgccggtg cgaatgcgca ccacctgctc
     6421 gatggacgtc acgaagatct ttccgtcgcc gatcttgcct gtctttgcag ccttgacgat
     6481 ggcctcgata cagcggtcga gatccgtgtc cttgatcatc acttcgatct tcaccttcgg
     6541 caggaaatcg acgacgtatt cggcaccccg gtacaactcg gtgtgcccct tctggcgccc
     6601 gaaccccttg acttcggtga cagtgaggcc ggtgacgccg acttcggcca gcgcctcccg
     6661 gacctcttca agcttgaatg gtttgagaat cgcggtgatc tgcttcatga agaattcctg
     6721 ctgcgtggat gggcacgagt ttaagcgcgg aatttcgatg tgatcggata gcgccagtcg
     6781 cgcccgaacg cccggtgggt gatgcggatg ccgacgggtg cctggcgccg cttgtactcg
     6841 ttgatcttga tcagccgggt gacccgttcc acatcagagc gagcgaaccc ggcggcgacg
     6901 atttcatcga tgctctggtc gttctccatg tagcgctcca cgacctcgtc gagcacggcg
     6961 tactcgggca ggctgtcctg gtctttctga tccgggcgga gttccgccga gggcgggcgg
     7021 gtcaggatcc ggttcgggat cacctcgccc cggccatcgc ctgcctggag gttcttccag
     7081 gcacacaagc ggtacaccag agtcttggcc acgtccttga tcactgcaaa gccgccggcc
     7141 atgtcgccgt agagcgtgca gtagccggtg gccatctcgc tcttgtt
//
LOCUS       DAOORV010000168         7186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12794_length_7186_cov_6.176413, whole genome shotgun sequence.
ACCESSION   DAOORV010000168 DAOORV010000000
VERSION     DAOORV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_7186_cov_6.176413"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="hflC"
                     /locus_tag="PKV56_15910"
     CDS             complement(<1..866)
                     /gene="hflC"
                     /locus_tag="PKV56_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOX69344.1"
                     /translation="MNRTGMYVIGALVALMVAFSTLFIVDQRQFAVIYALGEIKAVVT
                     EPGLKFKMPPPFQNVVFLDRRTQTLDSPETRPIFTAEKKSLVIDWLVKWRVTDARQFI
                     RNNGVDMRNVESRLSPIVQAAFNEEVTKRTVQAMLATDRDRVMQGVRARLSDEAKSFG
                     IEIVDVRIKRVDFSSNITDSVYRRMESERKRVANELRSTGAAEAEKIRADADRQREVI
                     VAEAYRDAQKVKGEGDARASTLYADSFGRDPQFAQFYRSLEAYRTSFRSKSDVMVLDP
                     SGDFFKAMRGNFG"
     gene            complement(886..2145)
                     /gene="hflK"
                     /locus_tag="PKV56_15915"
     CDS             complement(886..2145)
                     /gene="hflK"
                     /locus_tag="PKV56_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOX69345.1"
                     /translation="MKTSSPEASARPPSRLHAAVQRLSAPFLNKGEGPPDLDELWRDF
                     NKKLTGMFGGKGGGTRPPTPGDGGNFQPDMKSAGIGVGLIAGVAALLWLGSGFFIVQE
                     GQQAVVTSFGKYSHTSDAGFQWRMPYPFQAHETVPVTQLRSIEVGRSGVVQSTGLRDS
                     SMLTQDENIVDIRFTVQYRLKDSRAYLFENRNPDDAVVQAAESAVREIVGKSTMDSVL
                     YEQRDAIANELVKSIQTQADRLNTGILVVNVNVQNVQAPEQVQAAFDDAFKAGADRER
                     LKNEGQAYANDVIPKAQGTAARLREEAEGYKSRVVAQAEGDAQRFASVLVEYQKAPAV
                     TRDRLYIDTMQQVYSNVSKVLVDSRSNSNLLYLPLDKLIQQAGAASSPATAVLPSETT
                     PAAAAAPPGTLDVRSRDGQRSRDRDGR"
     gene            complement(2142..3392)
                     /gene="hflX"
                     /locus_tag="PKV56_15920"
     CDS             complement(2142..3392)
                     /gene="hflX"
                     /locus_tag="PKV56_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOX69346.1"
                     /translation="MNPDSSPAGSLNPARAILVGVAIGRCPQFDDTLDELALLAESAG
                     DLPVARVIARRKSPDPALFIGTGKADEIKALVALHQAEVVLFDQALGAAQQRNLERHM
                     GVAVADRTMLILEIFADRAQSHEGKLQVELARLQYLSTRLVRRWSHLERQRGGIGNRG
                     GPGEAQIELDRRMIGERIKSVKERLEKVKRQRNTQRRSRERSETFRVSLVGYTNAGKS
                     TLFNALVKARAYAADQLFATLDTTTRQLYLEEAGRNVSLSDTVGFIRDLPHKLVEAFE
                     ATLQEAAQADLLLHVIDAASPNRDEQRAEVQRVLASIGAADVPQILVFNKVDLLEQHQ
                     TPLLPVDEILLDDGRQVPRVFTSAAHGLGTDALRRLIAAAALRPPAVAESKPEAGPQI
                     SPLECDDMDVRLLPEPTGTLPLLA"
     gene            complement(3430..3672)
                     /gene="hfq"
                     /locus_tag="PKV56_15925"
     CDS             complement(3430..3672)
                     /gene="hfq"
                     /locus_tag="PKV56_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOX69347.1"
                     /translation="MSNKGQLLQDPFLNLLRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPGRAVNFHAAETPEST"
     gene            complement(3754..5085)
                     /gene="der"
                     /locus_tag="PKV56_15930"
                     /pseudo
     CDS             complement(3754..5085)
                     /gene="der"
                     /locus_tag="PKV56_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794569.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            complement(5117..6235)
                     /gene="bamB"
                     /locus_tag="PKV56_15935"
     CDS             complement(5117..6235)
                     /gene="bamB"
                     /locus_tag="PKV56_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HOX69348.1"
                     /translation="MSRVGGALVVALCAALSACSLAPEKPKPKEVVAVKPAIAGRVVW
                     KARTSDVEFPLSVAVNGNTFTVASSDGTVTALDADKGRQLWSGNAGGALSAGVGSDGR
                     VAAVVTRDGQVVALEAGKVLWKRPIATRVATAPLVAGERVFVVGVDRSVSAFDALNGA
                     KLWTVQRPGEALTLAQAGVVTAFKNTLLVGQGPRLAGLDPSSGAVRWEVPLASPRGSN
                     EVERLADVVGPAVRVGDIVCMRAFQAAVGCVNAERGSQVWTKNFGGRQGVAADAQYLY
                     AADGSDRITAWKASTGEVVWTSDAYLNRSLSTPFSLGPAIVFGDFEGYLHFLSADQGV
                     AQLRLETDGSPMAAPPALSGTTVLVVTREGGLFGLRPE"
     gene            complement(6232..6924)
                     /locus_tag="PKV56_15940"
     CDS             complement(6232..6924)
                     /locus_tag="PKV56_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX69349.1"
                     /translation="MATHLDLEEQEQLDAVKHFWKRYGNLITWLLILVLGGYAAWNGW
                     NWWQRDQATKAGAMFDELDRAAQAGDADKSNRVFNDLKDRYPATAFAQQGGLLSAKLQ
                     ADSGKPDLAQATLAWVASNAAETEYQTIARLRLAGLLLDEKKFDDALKQLDAATAPSF
                     ESLVADRRGDVLFAQGRVDDAKAAYSKAWLLMDPKVEYRRLIGAKLAALGASPVSAPD
                     PAAAAASSGASR"
     gene            complement(6954..>7186)
                     /locus_tag="PKV56_15945"
     CDS             complement(6954..>7186)
                     /locus_tag="PKV56_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOX69350.1"
                     /translation="AGVSVLMHAAGREGLGSMKSQFKRADASGARHALIFGDDEVARG
                     EVAVKPLRQADAAQRCLLMVQAGDWAAELRTA"
BASE COUNT         1345 a         2334 c         2153 g         1354 t
ORIGIN      
        1 ccgaagttgc cccgcatcgc cttgaagaag tcgcccgacg gatcaagcac catcacgtcg
       61 gacttgctgc ggaaactggt gcgataggct tccaggctgc gatagaactg cgcgaattgg
      121 ggatcgcgac cgaaagaatc tgcatacaac gtcgatgcac gggcgtcacc ctcgcccttg
      181 accttctgcg cgtcgcggta ggcttcggca acgatcactt cccgctgccg atcggcatcc
      241 gcgcggatct tctcggcctc tgccgcaccc gtggaacgca actcgttggc cacgcgcttg
      301 cgctccgatt ccatgcgccg gtaaacggag tcggtgatgt tggacgagaa atcgacgcgc
      361 ttgatgcgta cgtcgacgat ctcgattccg aacgacttag cctcgtccga gagacgcgcg
      421 cggacaccct gcatgacacg atcacgatca gtcgcaagca tcgcctgcac ggtgcgtttg
      481 gtcacctctt cgttgaatgc cgcctgcacg atcgggctca gacgactctc gacattgcgc
      541 atgtcgacac cgttgttgcg gatgaactga cgagcatccg tcactcgcca cttcaccagc
      601 cagtcgatga cgagactctt cttctcggcc gtgaagatcg ggcgagtctc cgggctgtcc
      661 agggtctgcg tgcgccgatc cagaaacacc acgttctgga acggtggcgg catcttgaat
      721 ttcagacctg gctctgtgac gacggccttg atctcaccca gtgcgtagat gaccgcgaac
      781 tgacgctgat ctacgatgaa caaagtggaa aaagcgacca tcaaggcgac caacgcgccg
      841 atgacataca tgcctgtgcg attcatgtgt tgcttccgtg aagtttcagc ggccgtcgcg
      901 atcgcggctg cgttggccgt cacgcgaacg cacatcgagc gtgccgggcg gcgcagcagc
      961 cgcggctggc gtcgtttctg agggaagcac cgcagtcgcc ggagaggagg cggcaccggc
     1021 ttgctgaatc agcttgtcga gtggcaggta aagcaggttc gaattgctgc ggctgtcgac
     1081 cagcaccttg ctgacattgg agtaaacctg ctgcatcgta tcgatgtaga gccgatcgcg
     1141 ggtgacagca ggagctttct ggtattccac cagaacgctg gcaaaccgct gtgcatcgcc
     1201 ctcagcctgc gcgaccacgc gcgacttgta gccttccgcc tcttcgcgaa ggcgagccgc
     1261 ggtgccctgc gccttgggga tcacatcgtt cgcgtaggcc tgaccctcgt tcttgagccg
     1321 ttcgcggtcg gcaccggcct tgaacgcatc atcgaaggca gcctgcacct gctctggagc
     1381 ctgtacgttc tggacgttga cattgactac cagaatgcca gtgttgaggc ggtcggcctg
     1441 tgtctggatc gacttcacca actcgttggc gattgcgtca cgctgctcgt acagcaccga
     1501 atccatcgtg ctcttgccga cgatctcccg caccgcggat tcagccgcct gcacgacagc
     1561 gtcatcaggg ttgcggttct cgaacaggta cgcacgcgaa tccttcagcc ggtactggac
     1621 ggtgaatcgg atgtcgacga tgttctcgtc ctgggtcagc atcgaagaat cgcgcagtcc
     1681 cgtggactgc acgaccccgc ttcgaccaac ttctatcgag cgcaactggg tgaccggcac
     1741 cgtttcgtgc gcctggaacg gataaggcat gcgccactgg aatcccgcat cggaggtgtg
     1801 gctgtacttg ccgaacgagg tgaccacagc ctgctgtccc tcctgcacga tgaagaagcc
     1861 gctgccgagc cagagcagcg ctgcgacgcc ggcgatcaat ccgacaccaa tgccagcgct
     1921 cttcatgtcg ggctggaagt tgcctccatc gccgggagtc gggggacggg taccaccacc
     1981 cttgccaccg aacatgccgg tcaacttctt gttgaaatcg cgccagagtt cgtcaaggtc
     2041 ggggggacct tcccccttgt tcaggaacgg ggccgacagg cgctgcaccg cggcatgcag
     2101 ccgggatggc gggcgagccg aagcttcggg tgagctggtc ttcatgcgag gagggggaga
     2161 gttccagtgg gttcagggag aagccgaacg tccatatcgt cgcattccag cggcgaaatc
     2221 tgggggccgg cttcaggttt cgattcagcg acagccggtg gtcgcaacgc cgcagcggcg
     2281 ataaggcgac gcaacgcgtc tgttcccaga ccgtgcgctg cacttgtgaa cacccggggc
     2341 acctgcctgc catcgtcgag gaggatctcg tcgaccggca aaagtggtgt ctggtgctgc
     2401 tcaagcaggt cgaccttgtt gaacaccagg atctgcggga cgtctgcggc accgatgctg
     2461 gccagcacgc gctgcacctc ggccctctgt tcatcgcgat tcggactggc agcgtcgatc
     2521 acgtgcagaa gcagatcagc ctgggctgcc tcctgcaagg tggcttcgaa ggcctcgacc
     2581 agcttgtgtg ggagatccct gatgaagccc accgtgtccg acaacgacac gttgcgaccg
     2641 gcctcctcca ggtagagctg tcgggtggtc gtatcgagtg tcgcgaacaa ctggtcggcg
     2701 gcataggctc gcgccttgac cagcgcattg aacagcgtgg acttgccagc gttcgtatag
     2761 ccgaccagag acacgcgaaa agtctcgctt cgctctcgcg accgacgttg cgtgttgcgc
     2821 tgcctcttga ccttctccag ccgctccttc accgacttga tgcgctcacc gatcatcctt
     2881 cgatcgagct cgatctgtgc ttcgcccgga ccaccccgat tgccgatgcc gccgcgctgc
     2941 cgctccaggt gactccatcg acgcaccagt ctggtggaaa ggtactgcag acgagcaagc
     3001 tcaacctgca acttgccttc atggctctgg gcgcggtccg cgaagatttc gagaatgagc
     3061 atcgtccggt cggccaccgc gacacccatg tggcgctcca ggttgcgctg ctgggccgca
     3121 ccgagtgcct gatcgaacag caccacctcg gcctgatgaa gggcaaccaa cgccttgatt
     3181 tcgtcggcct tgcctgtgcc gatgaacagc gcagggtcgg gagacttgcg gcgagcgatg
     3241 acgcgggcga ccggcagatc tccagccgac tcggccagca gagccaactc atcaagcgtg
     3301 tcgtcgaact gcgggcagcg gcctattgcg acgcccacca ggatggcgcg cgccggattg
     3361 agcgacccag caggcgagga gtccggattc aaaatgggaa cggggtccag ccggaaactg
     3421 tcgacgctct caagtcgact cgggcgtttc ggcagcgtgg aagttcactg cccgaccagg
     3481 aaccaccgtc gagatggcgt gcttgtagac catctgggtc acggtgttgc gcaacaggac
     3541 cacatactgg tcgaatgact cgatgtgccc ctgcaatttg atgccgttga ccaggtaaat
     3601 agacaccgga acgtgctcct tgcgaagcag gttcagaaac ggatcctgca aaagctggcc
     3661 tttgttgctc acgatatcct ccgtgttggt tggcgtgcga gcggaaacgt taacacagcg
     3721 attcgtgtcg atcacagcct agggcgattg ccctcatgct tttgcatcga acgggttctt
     3781 gccggaacgc atctctatgc gcaacggcgt tccggtcaac ttgaagtgct cgcggatgcg
     3841 cccttccagg aatcgcttgt aggcgtcggt gacgtgttcg agcgaattgc cgtgaacgac
     3901 cacgattggc ggattcatgc ctccctgatg cgcgtaccgc agcttggggc ggaacatgcc
     3961 ggcccgctgt ggcgtctggt gctcgaccgc ctcgagcagc agacgcgtca gcaccggcgt
     4021 gggcatcttg cgggtcgccg aagcgtgggc gtcggcgatg gctttctata ccggccccag
     4081 accttggcgc ttgatggccg agatgttcat gaccggcgcg aacttcaaga aagccagacg
     4141 ctgttcgatc gaccgctgca gcaattgctt ctggtaatcg tcgaccgcat cccacttatt
     4201 gaccgcaatg accaccgcgc gaccactctc caggatgtaa ccggcaatgt gcgcatcctg
     4261 ctcgctgacg ccctgcgtcg catcgagcag gagcagcacc acattcgcat cggcgatcgc
     4321 ctgcaaggtc ttgaccacag agaacttctc gattgcctcg aagaccttgc ccttgcggcg
     4381 caagccggcc gtgtcgatca actcgaactt ctggccattg cgctcgaacg gaacagtgat
     4441 cgcatcccgg gtggtacccg gcatgtcgaa tgccaccagg cgctcctcac ccagccaggt
     4501 gttgatgaga gtcgacttgc ctacgttcgg gcgcccggcc acagccaatc ggatcggtgc
     4561 atcctcttgc gcctcgggct cgtcctcgac aaagtcgaaa cgtgccaggg ccgcctcggt
     4621 caggctgcga atgccttggc cgtgcgccga cgagatcggg tggggctcac ccatgcccag
     4681 ttcatagaat tcgccaagaa gcggcgaatc gaccatgcct tcggccttgt tcgcggccag
     4741 caccaccgtc tttccgacgg ttcgcaaata tcgcgcgatg tcgtgatcct gggcagacag
     4801 gccggcacgg atgtctacga cgaagaccac cgcatccgcc tcggccacgg cctggcgcgt
     4861 ctgctttgcc atctccttga cgatgccgct gtcgctggtc ggctcgaagc caccggtatc
     4921 gacgacgatg aactcgcggt cgccgatgcg cccgtcaccg tagtggcggt cgcgggtcag
     4981 gccggcgtaa tccgcgacga tcgcgtcgcg ggtcttggtc atgcggttga agagcgtcga
     5041 tttgccgacg ttcggccgcc cgacgatggc aatgaccggc ttcatggaca ccaactgacg
     5101 ttcaggaccg cctgcgtcat tccggtcgca ggccaaacag cccgccttct cgggtcacca
     5161 ccagcaccgt ggtcccagat agcgcaggcg gcgcagccat cggactgccg tcggtttcca
     5221 gcctcaactg ggcaacgcct tgatctgcag acagaaagtg caaatatcct tcgaaatctc
     5281 caaacacgat ggccgggcca aggctgaacg gcgtcgacaa gctgcggttc aggtacgcat
     5341 ccgaggtcca gaccacctca ccggtcgaag ccttccatgc ggtgattcga tccgaaccgt
     5401 cggccgcata gaggtactgc gcatcggccg caacgccctg gcgcccgccg aaattcttgg
     5461 tccaaacctg actgcctctc tccgcgttca cgcagccgac agcagcctgg aaagcacgca
     5521 tacagacaat gtcgccgacg cgaactgccg ggccaaccac atcggcaagg cgctcgactt
     5581 cgttcgaacc acgcggagat gccagcggca cctcccatcg aacggctcca ctggacggat
     5641 cgaggccagc gagtcgcgga ccctgtccca ccagcaatgt gttcttgaac gcggtcacca
     5701 cgccggcctg ggccaatgtc agcgcttcgc ccggacgttg cacggtccac agcttcgccc
     5761 cgttcagcgc atcgaaagcc gacacgctgc gatcgacccc cacgacgaat acgcgttctc
     5821 cagcgaccag gggagccgtg gcaactcggg tagcgatggg acgcttccag agaaccttgc
     5881 cggcctccag ggccaccacc tgaccatcgc gcgtcaccac cgccgccacc cgaccgtcgc
     5941 tgccaacacc ggcactcagc gcaccaccgg cgttgccact ccaaagctga cgccccttgt
     6001 cggcgtcgag cgcggtgacc gtgccatccg acgacgccac cgtaaaggtg tttccattga
     6061 ccgccactga cagcggaaac tccacatcgg acgtgcgcgc cttccagacc acgcggccgg
     6121 cgatcgccgg tttgacggcg accacctcct tgggtttcgg cttttccggc gccagcgagc
     6181 aggccgagag cgcagcgcac agggcgacca ccaaagcccc gccgacgcga ctcatcgcga
     6241 ggctcctgag ctggcagcag ctgcggctgg atccggggcc gataccggag acgcacccag
     6301 cgcagcgagc ttggctccaa tcagccggcg gtattcgact ttcgggtcca tcaacagcca
     6361 ggccttgctg taggccgcct tcgcatcgtc caccctgccc tgcgcgaaca agacatcgcc
     6421 cctgcgatcg gcgaccaggg attcgaagct gggggcggtg gctgcatcga gctgcttcaa
     6481 tgcatcgtcg aacttcttct cgtccagcag caagcctgca agcctcagcc gggcgatcgt
     6541 ctggtactcg gtctcggcgg cattcgaggc aacccaggcc agcgtggcct gagcgaggtc
     6601 cggcttgccg ctgtcggcct gcagtttggc ggacagcaaa ccgccctgct gcgcgaacgc
     6661 agtcgccgga tatctgtcct tcaggtcatt gaagacgcga ttgctcttgt cagcatcgcc
     6721 ggcctgagcg gcccgatcga gttcgtcgaa catcgcgccg gcttttgtcg cttgatctcg
     6781 ttgccaccag ttccatccat tccaggccgc atagccgccc agcaccagga tcagcaacca
     6841 ggtgatgagg ttgccgtagc gcttccagaa gtgcttgacc gcatcaagct gttcttgttc
     6901 ttcaaggtcg agatgcgtgg ccatgcgtca gatgagtttc gggtaacagc gaattatgcg
     6961 gtgcgcagtt ccgcagccca gtcgccagcc tgcaccatga gcaggcatcg ctgggctgcg
     7021 tcggcctgac gcaatggctt gacggcgact tctccgcgcg cgacttcgtc gtcgccaaag
     7081 atcaaagcat ggcgcgcacc actggcatcg gcgcgcttga actgggactt catgcttccc
     7141 agaccttcgc ggcccgcggc gtgcatcaga acagatacgc cggctg
//
LOCUS       DAOORV010000169         7161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12855_length_7161_cov_7.619476, whole genome shotgun sequence.
ACCESSION   DAOORV010000169 DAOORV010000000
VERSION     DAOORV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_7161_cov_7.619476"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKV56_15950"
     CDS             complement(<1..880)
                     /locus_tag="PKV56_15950"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOX69351.1"
                     /translation="MKFTRRQTLAAALGSLGAGVLPRAQAQTQPFPSQVIKFVIPTPA
                     GGDHDTMMRIIGQKLTDAWGQPCIVESRSGASGAIAAATVSQAPADGYTVLIGYSALL
                     SNTVLMPKPGYRLEDLQPVGMLALTPIAFGARESLGASTLKQYVALAKTRPGKLSYGS
                     YGPGSGGHFVGELFNMAAGIDTVHVPYKGQPPALQDLIGGQIDAAVASLGNVNRYPGK
                     IKPLAVASATRFPSYPDVPTFAELGYPEVDMLGWAAAFVPAKTPKAIVDKLNTEMGRI
                     LVMPDVRAKLLELGFEP"
     assembly_gap    1084..1183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1184..1445)
                     /locus_tag="PKV56_15955"
     CDS             complement(<1184..1445)
                     /locus_tag="PKV56_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOX69352.1"
                     /translation="MGKWLQSNEHLTFEVRERVARITLNRPDRRNALSWEMLSELRAA
                     LLEADDLRSVSVIVLGGAGPDFCAGYDMKSAYARYAAEGDAAL"
     gene            complement(1456..2988)
                     /locus_tag="PKV56_15960"
     CDS             complement(1456..2988)
                     /locus_tag="PKV56_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOX69353.1"
                     /translation="MSITPMIYLDGRGVTRDEVLRVAQQAATGFEQLGVSEGDTVALL
                     LRNDFAFFEVQQAAAAVGAYGVPLNWHGRTEEVIYVLEDAKPKVLVAHADLLAPIRAL
                     VPAGIRVFVVPTPPEIQQRYGVSTELARPLPGDTVWREWAERFEPLQAPPRRARATMI
                     YTSGTTGHPKGVKRQLATPEESKAYVELFASVYGLKPGMRALVCGPLYHASPNGYGRQ
                     ALMTADVLVLQSKFDPEETLALIEKHRIDNAVMVPTMFVRMLKLPPEVRNRYDVRSLR
                     WVTHTGAPCPREVKKDLMDWWGPVVYEGYGGTEVGTATLATPQDWLEHPGCVGMPTPG
                     TRMAFYDEEGNRLPEGEIGEIFARVPAYPDFTYLNHEEKRKSVERDGLISLGDVGYMK
                     EGRLYLCDRRSDMVIFGGTNIYPAEIEMVLTQCPGVKDCAVFGIPDDDFGESLAAAVE
                     RMPESQVTPEEIRSYLLERLAKFKVPKRIDFHESLPREDSGKIFKRRLRDPFWQNAGR
                     KI"
     gene            complement(3000..4136)
                     /locus_tag="PKV56_15965"
     CDS             complement(3000..4136)
                     /locus_tag="PKV56_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOX69354.1"
                     /translation="MNFELNEDQAALAESLSRLLADRYGFEKRRALTASGAAHDEKTW
                     AGLAELGLTALPVPEALGGFGGGARDLLPAMQAFGRSLSMEPFLASAVLAGTALRLGA
                     DEAMQTRLLPGMASGEIVSAWAHDEPAGRHAPLWVETRAERKDGGWKLDGIKSNVPAG
                     GLARYFVVSARIAGAPADANGCALFLVDAKDAGVALREFRLVDDTAAGELVLTQAAAQ
                     PLANPEDSTRTRLAIQGTVAAGIAAVCADMSGAAQAAFDLAMDYLRTRKQFGRLIGEN
                     QALRHRASEMLVSLEMARSMAMAAAVAADDASAEDSAPDLHRAKLSVARNARNVALGA
                     IQLHGGIGMTEEYAVGHYLRRIHVLDQMFGDADAHAARLAAALL"
     gene            complement(4144..5304)
                     /locus_tag="PKV56_15970"
     CDS             complement(4144..5304)
                     /locus_tag="PKV56_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOX69355.1"
                     /translation="MDLDLTPEQRAFRDEVRTFIREKLPSEIRERLRAGHPPRKQDTV
                     AWQRILNERGWAAPHWPKRYGGADLGPMERLILLDEAYRAPAPLPQAFNVGMLGPVLM
                     KFGTPAQCDHFLPRLANLDIWFCQGFSEPGSGSDLASLRTAARREGDHYIVNGQKIWT
                     TTAHLADWVFALVRTDTEARKQEGISFLLIDLRTPGITIRPIHSIDGEHHLNEVFFDE
                     VRVPVGNLVGQENKGWDCAKFLLSNERTGIANVGLCRERLDYARELAGRYTQAGKPLL
                     DDPKLRADIAMLEAETRALEITNWRFLLTDSEQKRLPAFASVLKLKGTELQQEINALL
                     ARIVGPGGLERRSADEVTHGPIAPRYFLSRAASIYGGTTEVQKDILAKAIVG"
     gene            5370..5966
                     /locus_tag="PKV56_15975"
     CDS             5370..5966
                     /locus_tag="PKV56_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69356.1"
                     /translation="MTNESLFQPVRTRRGFEAVCDRIREQLMSGELKPGDQLPGEREL
                     AERFGIGRAAARDALRSLEVSGVVEARKGIQGGFFIRNGGSGGLAQTVQDMVSLAQAS
                     LENVMEARTEIICVAIRLACPRATNEEFDTIQRDIDLHADLFRLGRASRNTQSVIEFY
                     RLIAQATHNPLVLMMVDALSEVYRGLLVFSAISDGTKS"
     gene            6021..>7161
                     /locus_tag="PKV56_15980"
     CDS             6021..>7161
                     /locus_tag="PKV56_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOX69357.1"
                     /translation="MAASYLPYEPQQQRLLPDALQDWLPEGHLAYFISDSVDSLDLSA
                     FHARYAKGGPRNQPFHPAMMIKVLIYGYATGTFSSRKLAAKLHEDVALRVLAAENYPA
                     HRTLSDFRALHLQELSALFVQVVRLARECGLVKLGTVAVDGTKLKANASRHKAMSYER
                     MLKAEAELKAQIDGLLNRAKAADDLEKNEPDIDIPSEIKRRADRLSAITAAKLRLEQR
                     QQEADAARGRSKDDQRKPRDKDGKPKGGRYKRDFGVPKDSAQESFTDTDSRIMKRAGG
                     GYDYSYNAHTAVDETAQIVVAAELTNNAADNDRLPVLLAAVKATLGDDAKQVLADAGF
                     RSESVFERLKDSPSELIVALGREGKQDLEIDAQKYPHSAAMDAKLK"
BASE COUNT         1217 a         2263 c         2394 g         1187 t
ORIGIN      
        1 ccggctcgaa gccgagctca agcagcttcg cgcgcacatc cggcatcacg aggatgcggc
       61 ccatctccgt gttgagcttg tccacgatcg ccttcggtgt cttggccggc acgaaggcgg
      121 cggcccagcc cagcatgtcg acctccggat agccgagttc ggcgaaggtc ggcacgtcgg
      181 gatacgaagg aaagcgcgtg gcgctggcaa cggcaagagg cttgatcttg ccggggtagc
      241 ggttcacgtt gccgagcgac gccactgccg cgtcgatctg tccgccaatg aggtcctgca
      301 acgcgggcgg ctggcccttg tacggcacgt gcacggtgtc gatgccggct gccatgttga
      361 acagctcgcc gacgaagtga ccacccgagc ctggaccata ggagccatag gagagcttgc
      421 cgggccgtgt cttggcgagc gccacgtatt gcttgagcgt cgaggccccc aggctctcgc
      481 gagcgccgaa cgcgatcggc gtgagagcca gcattccgac cggctgcagg tcttcgagcc
      541 gatagcccgg cttcggcatc agcaccgtgt tgctgagaag cgcgctgtag ccgatcagca
      601 cggtgtatcc gtccgccggc gcctgcgaca ccgtggctgc ggcgatggcg ccgctcgcac
      661 cggacctgga ttcgacgatg cagggctggc cccaggcgtc ggtcagcttc tggccaatga
      721 tccgcatcat ggtgtcgtgg tccccgcccg ccggcgtagg aatcacgaac ttgatgacct
      781 gtgaagggaa gggctgcgtc tgcgcctgcg cgcgcggcag aacgcccgca ccgagcgagc
      841 ccagggctgc agcaagcgtc tggcgtcgag tgaacttcat tgcgatgtct ccttgtgtga
      901 tggatctggc tgccatacga ttcgtcgaaa tcattggttc aaccatagat taaactacaa
      961 actgaaaaat tcatccaccg caacattcac gacagcaagt gcgctcgacg cggcgcagac
     1021 gagcgcgctc gatgcgttga tccgccgcga gatcggtgcc ttcgatggcg cgctgagggt
     1081 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagcgc ggcatcgcct
     1201 tcggcggcgt agcgcgcgta ggccgacttc atgtcgtagc ccgcgcagaa atcggggcct
     1261 gcgcctccga gcacgatcac gctcacgctc cgcaggtcgt cggcctccag cagcgctgcg
     1321 cgcagctcgg agagcatctc ccacgacagc gcattgcgcc ggtccggccg gttgagggtg
     1381 atgcgcgcga cgcgctctcg cacctcgaag gtgaggtgtt cattggattg cagccacttg
     1441 cccatgcgcc gcccctcaaa tcttgcgccc ggcgttctgc cagaacgggt cgcgcaagcg
     1501 gcgcttgaag atcttgccgc tgtcctcgcg cggcagcgat tcgtggaagt cgatccgctt
     1561 gggcaccttg aacttcgcga gacgttcgag aagataggac cggatctcct ccggcgtgac
     1621 ttgcgactcg ggcatgcgtt cgacagcggc agcgagcgat tcgccgaagt cgtcgtcggg
     1681 aatgccgaac acggcgcagt ccttcacgcc agggcactgg gtcagcacca tctctatctc
     1741 ggccggatag atgttggtgc cgccgaagat caccatgtcc gaacggcggt cacagagata
     1801 gagccggcct tccttcatgt agcccacgtc gcccaggctg atgaggccgt cgcgctccac
     1861 gctctttcgc ttctcttcgt ggttgagata agtgaagtcg ggatacgctg gcacgcgagc
     1921 aaagatctcg ccgatctcgc cttcgggcag gcgatttccc tcctcgtcat agaaggccat
     1981 gcgcgtgccg ggcgtgggca tgccgacgca accgggatgc tccagccagt cctgcggcgt
     2041 ggcgagcgtg gcggtaccga cttcagtgcc gccgtagccc tcgtagacca cgggacccca
     2101 ccaatccatc agatccttct tcacctcgcg cggacacggc gcgccggtgt gcgtcaccca
     2161 tcgcagcgag cgcacgtcgt agcggttgcg cacctccggc ggcagcttga gcatgcgcac
     2221 gaacatcgtg ggcaccatca ccgcgttgtc gatgcggtgc ttctcgatca gcgccagcgt
     2281 ctcttccgga tcgaacttgg actgcagcac cagcacgtcc gccgtcatca gcgcctgacg
     2341 gccgtacccg ttgggcgacg cgtggtagag cgggccgcac accagcgcac gcatgccggg
     2401 cttgagccca tacacgctgg cgaacaactc gacataggcc ttgctttcct cgggcgtggc
     2461 gagctggcgc ttcacgccct tgggatggcc ggtggtgccg gaggtgtaga tcatggtcgc
     2521 gcgggcgcgg cgcggcggcg cctgcagcgg ctcgaagcgc tcggcccatt cgcgccaaac
     2581 cgtgtcgccc ggtaagggcc gtgcgagttc ggtcgagacg ccgtagcgct gctggatttc
     2641 cggcggcgtc ggcaccacga agacgcggat gcccgcgggc acgagcgcgc ggatcggggc
     2701 gagaaggtcg gcatgcgcga ccagcacctt cggcttcgcg tcttcgagca cgtagatcac
     2761 ttcttccgtg cggccgtgcc agttgagcgg cacgccatag gcgccgaccg cggcggcggc
     2821 ctgctgtact tcgaagaagg cgaagtcgtt gcgcagcagc agggcgacgg tgtcgccttc
     2881 gctcacgccg agttgctcga agcccgtggc tgcctgctgc gccacgcgca gcacctcgtc
     2941 gcgcgtcacg ccgcggccgt cgaggtagat catgggggtg atgctcatcg gtgtaggtct
     3001 caaagaaggg cagccgcgag acgcgcggca tgggcgtcgg cgtcgccgaa catctggtcg
     3061 agcacgtgga tgcgccgcag gtagtgcccg acggcgtatt cctctgtcat gccgatgccg
     3121 ccgtgaagct ggatcgcgcc gagcgccacg ttgcgtgcgt tgcgggcgac ggagagcttc
     3181 gcgcgatgca ggtcgggggc actgtcttcc gcgctggcat cgtctgccgc aacagcggcg
     3241 gccatggcca tgctgcgcgc catctcgagg ctgaccagca tctcggacgc gcgatggcgc
     3301 agcgcctggt tctcgccgat caggcggccg aactgcttgc gtgtgcgcag gtagtccatc
     3361 gcgagatcga aggcggcctg ggcggcgccg ctcatgtcgg cgcacacggc ggcgatgcca
     3421 gcggccacgg tgccttggat ggcgagacgc gtgcgagtcg aatcttcggg gttggccaac
     3481 ggctgcgccg ctgcttgcgt cagcaccagc tcgcctgctg ctgtgtcatc gaccaggcgg
     3541 aactcgcgca gcgcgacgcc tgcgtccttg gcgtccacga ggaacagcgc gcacccgttc
     3601 gcgtcagccg gtgcaccggc gatgcgcgcg ctcacgacga agtaccgcgc cagacctccg
     3661 gcgggcacgt tggacttgat gccgtcgagc ttccagccgc cgtccttgcg ttcggcacgc
     3721 gtctccaccc acagcggcgc gtgacggccg gcgggttcgt catgcgccca ggccgacacg
     3781 atctcgccgc tggccatgcc gggcagcagt cgtgtctgca tcgcctcgtc ggcgccgagg
     3841 cgcagggcgg tgcctgcgag caccgcgctc gccaggaacg gctccatcga gagcgagcgg
     3901 ccgaaggcct gcatcgcggg cagcaggtcg cgcgcaccgc caccgaagcc gccgagtgct
     3961 tctggcactg gcaacgctgt caggcccagc tcggccaatc cagcccaggt cttttcgtca
     4021 tgcgctgcgc cggaggcggt gagcgcacgg cgcttctcga atccatagcg atcggccagc
     4081 aggcgggaaa ggctctccgc gagcgccgcc tgatcctcat tcagttcgaa attcatgttt
     4141 gccttatccg acgatggcct tggccaggat gtccttctgc acttcggtcg tgccgccgta
     4201 gatcgacgct gcgcgcgaaa ggaaatagcg cggcgcaatc ggaccgtgcg tgacttcgtc
     4261 ggcggagcgg cgctccagac cgcccgggcc gacgatgcgt gcgagaagcg cgttgatctc
     4321 ctgctgcaac tcggtgccct tgagcttgag taccgacgcg aaggccggca ggcgcttctg
     4381 ctcgctgtcg gtgagcagga agcgccagtt ggtgatctcc agcgcgcgcg tctcggcctc
     4441 cagcatggcg atgtctgcgc gcagcttggg gtcgtcgagc agcggcttgc cggcctgtgt
     4501 gtagcgtccg gcgagttcgc gcgcgtagtc gagccgctcg cggcacaggc cgacgttggc
     4561 gatgcccgtg cgttcgttcg acagcaggaa cttcgcgcaa tcccagccct tgttctcctg
     4621 gccgaccagg ttgccaacgg gcacgcgcac ctcgtcgaag aagacttcgt tgagatggtg
     4681 ctcgccgtcg atggagtgga tcggccggat cgtgatgccg ggggtgcgca ggtcgatcag
     4741 caggaaagaa atgccttcct gcttgcgcgc ctcggtatcg gtgcgcacga gggcgaacac
     4801 ccagtcggcg aggtgcgcag tggtggtcca gatcttctgg ccgttgacga tgtagtgatc
     4861 gccctcgcgg cgtgcggcag tgcgaagcga cgcgaggtcg gagccggagc cgggctccga
     4921 gaagccctgg cagaaccaga tatcgagatt cgcgaggcgc ggcaggaagt ggtcgcactg
     4981 tgcaggcgtg ccgaacttca tcagcaccgg gccgagcatg ccgacgttga acgcctgcgg
     5041 cagcggggcg ggggcgcgat aggcttcgtc gagcaggatc aggcgttcca tcgggccgag
     5101 gtcggcgccg ccgtagcgct tgggccagtg cggcgcggcc cagccacgct cgttgaggat
     5161 gcgctgccag gccactgtgt cctgcttgcg cggcggatgg ccggcacgca ggcgttcgcg
     5221 gatctctgag ggcagctttt cgcggatgaa ggtgcgcacc tcgtcacgga aggcgcgctg
     5281 ttcgggggtg aggtccaagt ccatggcgaa tcatcttata gttcaaccaa tgaatatgca
     5341 atactgccga accctcaaaa ggatctttca tgaccaacga atcgctcttc caacctgtgc
     5401 gcacccggcg tggcttcgag gccgtgtgcg accgcattcg cgaacagttg atgagcggcg
     5461 agctgaagcc gggcgaccag ctccccggcg agcgcgaact ggccgagcgc ttcggcatcg
     5521 gacgcgcggc agcacgcgac gcattgcgca gcctggaggt ctcgggcgtg gtggaagcgc
     5581 gcaagggcat ccagggcggc ttcttcatca ggaatggcgg cagcggcggt cttgcgcaga
     5641 cggtgcagga catggtgtcg ctcgcgcagg cgtcgctgga gaacgtgatg gaggcgcgca
     5701 ccgagatcat ctgcgtggcg atccgcctcg cctgcccgcg cgcgaccaac gaggagttcg
     5761 acacgatcca gcgcgacatc gacctgcatg cggatctgtt ccgactgggg cgcgcctcgc
     5821 gcaacacgca gtcggtgatc gagttctacc ggctcatcgc gcaagcgacg cacaacccgc
     5881 tggtgctgat gatggtggac gcgttgtctg aggtgtaccg gggtctcctc gttttcagtg
     5941 caataagtga cggtacgaaa agctaggagc ttgcgcggca gaagcgcact gaggtcgcga
     6001 aggcttggta aattgtgggc atggccgcaa gttacctccc ctacgaaccc caacagcagc
     6061 gactgctgcc cgacgcactg caagactggc tgcccgaagg gcacctggcg tacttcatca
     6121 gtgacagcgt cgacagtctg gatctgagcg cgtttcacgc ccgatacgcc aagggcggtc
     6181 cgcgcaatca accgtttcac ccggccatga tgatcaaggt cttgatctat ggctacgcca
     6241 cgggtacctt cagctcgcgc aagctggcgg ccaaactgca tgaagacgtg gcgctgcggg
     6301 tgctggcggc agagaactac ccggcgcacc gcacgctgtc cgattttcgg gcactgcacc
     6361 tgcaagagct ctcagccttg ttcgttcagg ttgttcgcct ggcgcgtgag tgcggattgg
     6421 tcaaactggg caccgtggcg gtggacggca ccaagctcaa ggccaatgcg agccgtcaca
     6481 aggccatgag ctacgagcgc atgctcaagg ccgaggccga actcaaagcc cagattgacg
     6541 gcctgctcaa ccgagcgaag gcggccgacg atctggagaa gaacgagccc gacatcgaca
     6601 tccccagcga gatcaagcgc cgggccgatc gcctgtccgc catcacggcg gccaagctgc
     6661 ggctggagca gcgccagcaa gaagccgatg ccgcgcgcgg acgcagcaag gacgaccagc
     6721 gcaaaccccg ggacaaggac ggcaaaccca agggcgggcg ctacaagcgc gacttcggcg
     6781 ttcccaagga cagcgcccag gagagcttca ccgacacgga cagccgcatc atgaagcgcg
     6841 caggtggtgg ctatgactac agctacaacg cgcacacggc ggtggacgag accgcgcaga
     6901 tcgtggtggc ggccgagctc accaacaacg ccgccgacaa cgatcgcctg cccgtattgc
     6961 tggcggcagt gaaggccacc ctgggcgatg acgccaagca ggttctggcc gatgcggggt
     7021 tccgatcgga gtcggtgttc gagcgactga aggacagtcc gagcgagttg atcgtggcgc
     7081 tgggtcgcga gggcaagcag gaccttgaga tcgacgccca gaagtacccg cacagcgcag
     7141 cgatggatgc caagttgaag a
//
LOCUS       DAOORV010000170         7129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_12935_length_7129_cov_7.484026, whole genome shotgun sequence.
ACCESSION   DAOORV010000170 DAOORV010000000
VERSION     DAOORV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_7129_cov_7.484026"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1202
                     /gene="motB"
                     /locus_tag="PKV56_15985"
     CDS             246..1202
                     /gene="motB"
                     /locus_tag="PKV56_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOX69358.1"
                     /translation="MAGDSKKLQPIIIKKIKKGGHGHHGGAWKIAYADFVTAMMAFFL
                     LMWLLGSTTEGDKKGIADYFGSPLKVSLGGGSGAGDASHVIKGGGEDLSRTHGQVKKG
                     DIESQRTTINLLALKAEQRAAEKARLEELKQRVEDVLAASPKLASMQSQIRLDMTSEG
                     LRIQIVDADNRPMFDSGSSIVKPYMRELLQIIGGVLGDVPNRLTLEGHTDAQPFGSGE
                     RGYSNWELSADRANSSRRELISGGLPDERVLRVQGLSSSRPLDNTDPLSPTNRRISII
                     VMNRDAEDRFFNNLSEPQDDTPVAEEPKIAVDLPSISAPVAR"
     gene            1244..1642
                     /gene="cheY"
                     /locus_tag="PKV56_15990"
     CDS             1244..1642
                     /gene="cheY"
                     /locus_tag="PKV56_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HOX69359.1"
                     /translation="MSQQTDMKFLIVDDFSTMRRIVRGLLKEIGYNNAEEAEDGAVAL
                     GMLNNIKFDFIVSDINMPNMNGFELLSAVKKDAALKHIPVLMVTAEARKEDIVRAAQD
                     GAAGYIVKPFTKATLEEKVQKIMQKLAATA"
     gene            1695..2303
                     /locus_tag="PKV56_15995"
     CDS             1695..2303
                     /locus_tag="PKV56_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOX69360.1"
                     /translation="MTSHAASAAASTEVFQQLGAITRQLHDTLSQLGVMPKLQIAAEG
                     LPDARSRLNYIAEKTGAAANKVLNSVDCAKADHLLITQETRRIAAAIVADPVKAVASG
                     MLMNFVGDVESATTRIDGHLTDIMMAQDFHDLTGQVVAKVVALASELEDSLVRLLVQA
                     APPDQVQKVEQHLQGPVVDAEGRTDVVANQGEVDDLLASLGF"
     gene            complement(2383..3027)
                     /locus_tag="PKV56_16000"
     CDS             complement(2383..3027)
                     /locus_tag="PKV56_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX69361.1"
                     /translation="MINVMLCDDHALIRRGIRDTLSDAPDIQVVGEAGDYGELRSLLR
                     NASCDVLVLDINLPGRSGLDVLHALKEEGSTLRVLVVSMYPEDQYAIRALRAGAFGYV
                     NKGGDPALIVAAVRTVAQGRKYVTPEIAQMLVESLTTPTSEAAHQKLSDRELQTLVMI
                     ASGKRLSDIAEELMLSPKTVSVYRARVLEKLGLANNSEMTVYAIRNGLVGGGVG"
     gene            complement(3035..4171)
                     /locus_tag="PKV56_16005"
     CDS             complement(3035..4171)
                     /locus_tag="PKV56_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX69362.1"
                     /translation="MASRSADQPLRVLHLEDSELDHELMLAHLRRGGLVVEPRRVDNE
                     ADFLRALDDRWDAIISDYNLPGFSGLVALDLLMARGGDVPFILVSGEIGEDTAVEAMR
                     NGASDYLLKNNLARLVPALLHAVEASETRRARQRADRQLGESQLRLHELAQHLQTSIE
                     MERAAIAREIHDDVGGSLTALKFDLAWIARHSDSPEVQARVQSALETVTAAIESSQRI
                     MHNLRPAILEQGLLAALQWMTARFERRTGIACALRAPQQPMPLGPGVPLVAYRTAQEA
                     LTNVSKHAQATQVSIDLSMAGGVLSLEISDNGRGLSSEDLAKARSFGIRGLHERARTV
                     GGWVDLSSGPTGTTLILSVPLDGAPGGSPDNPADADDPTGWGDL"
     gene            4420..4899
                     /locus_tag="PKV56_16010"
     CDS             4420..4899
                     /locus_tag="PKV56_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69363.1"
                     /translation="MEYAGHQVEPTQVGQAPAPLLVAADLQDHLMMATNDLDRLHTLL
                     ADACDTLMQRFYNATEQIHTLMDEHSSDGDDKRDSAAYQNLMQQLGAAVTALQFQDMA
                     SQLIVHTNHRLRSCVDTLAQEAFGDDDEDGQSAVAAPPLRPNPVTQSEMDAGSVELF"
     gene            4966..5328
                     /locus_tag="PKV56_16015"
     CDS             4966..5328
                     /locus_tag="PKV56_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69364.1"
                     /translation="MHSILAVDDSASMRQMVTFTLKSAGYHVVEAVDGQDALEKSGQQ
                     DFDLVLTDQNMPRMDGINLTKNLRNHPKFKATPILILTTESSDQMKQAGRNAGATGWM
                     VKPFDPAKLIEVIRKVIR"
     gene            5381..>7129
                     /locus_tag="PKV56_16020"
     CDS             5381..>7129
                     /locus_tag="PKV56_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOX69365.1"
                     /translation="MSDMNTDQSSGAGIDLSQFYQVFFEEAGENLDSMEQQLLAIDIE
                     AADDEELNSIFRCAHSVKGGAATFGFADVAELTHQMETLLDRLRRHELEPTAPMVDVL
                     LQSGDALRSQLARHQGAGGDPVDTTDLLVKIRALVAGQPLPASAAAAAPASAAPKSVL
                     AQEPVASSAPAAPAKSAPAAKMARGLELRVGPLTDPKDADNLVDLFKEIVDLGTIEPL
                     DGGQASEGLRRFKVVTTSSDDDLLDLFTFHVAREKVALLPLTSGFGFHEGAPGAPPVE
                     NPPDPGFGFFEDAPGAPGSASPTAASTSNSPTAPIASESASAAAPARAPAKTEKAPAA
                     ALESSTLRVSVEKVDQLINLVGELVITQAMLAQNSKQIDTALYQQLAAGLADLDRNTR
                     DLQESVMSIRMIPMSMVFSRFPRMLRDLAAKLGKKVDFVTQGEATELDKGLVEKITDP
                     LTHLVRNSCDHGIEMPADRLAKGKPETGTITLAASHQGGSIVIEVRDDGRGLSREKLI
                     KKARERGIDAPDSMSDSEVWNLIFAPGFSTADVVTDVSGRGVGMDVVKKNITSLGGTV
                     EIESAEGYGMRVSVRLP"
BASE COUNT         1370 a         2349 c         2259 g         1151 t
ORIGIN      
        1 ggtcgatgcc ctgacccgct cgatggcggt cgagctggga ccacatggca tccgcaaggt
       61 gttgttctcg accgaccgac cgaccttcgc cgagctggaa agccacgtca agggcaagaa
      121 gtgagccccc cagtcgcttt gctcctgccc ccagggggcg ccacccatcg ggccgggcga
      181 cccgtccttc gggcgtggct tgacgaacgc gcagttgcgc ccgcttttct tggcgggagc
      241 tgctgatggc gggcgattcg aagaagctcc agccgatcat catcaagaag atcaagaagg
      301 gcggccacgg tcatcacggg ggcgcctgga agatcgcgta tgccgacttc gtgaccgcca
      361 tgatggcgtt cttcctgctg atgtggctgc tgggctcgac caccgaaggc gacaagaagg
      421 gcatcgccga ctacttcggc tcgccgctga aggtgtcgct cggtggcgga tcgggtgctg
      481 gcgatgcgtc gcacgtgatc aagggcggcg gcgaggacct gtcgcgcacg catggtcagg
      541 tcaagaaggg cgacatcgag agtcagcgca ccacgatcaa cctgctggca ctgaaggcag
      601 aacaacgcgc cgcggagaag gcccgcctcg aggaactgaa gcaacgggtc gaggatgtgc
      661 tcgcggccag cccgaagctg gcgtcgatgc agtcgcagat ccggctcgac atgaccagcg
      721 agggcctgcg catccagatc gtcgacgccg acaaccgtcc gatgttcgac agcggaagct
      781 cgatcgtcaa accctacatg cgcgaactgc tgcagatcat cggaggcgtt ctgggcgacg
      841 tgcccaatcg cctgaccctg gagggccaca ccgatgcgca accgttcggc agcggggagc
      901 gcggctacag caactgggag ctgtcggccg atcgtgccaa ctcctcgcgg cgtgaactga
      961 tctccggagg tctgcccgat gaacgcgtgc ttcgagtgca gggcctgtcg tccagcaggc
     1021 cgctggacaa caccgacccg ctgagcccga ccaaccgccg catcagcatc atcgtgatga
     1081 accgtgatgc cgaagacagg ttcttcaaca acctgtctga accgcaagac gacacaccag
     1141 ttgcagagga accgaagatt gcggtggatc tcccctcaat ttcagctccc gttgcccgat
     1201 agatctgact agcccacacc gattgcgtat tgcgaggacc gccatgagcc aacagaccga
     1261 catgaaattc ctgatcgtgg acgacttctc cacgatgcgc cgcatcgtgc gcggcttgct
     1321 gaaggagatt ggctacaaca acgccgagga agccgaggac ggcgccgtgg cgctggggat
     1381 gctgaacaac atcaagttcg atttcatcgt cagcgatatc aacatgccga acatgaacgg
     1441 cttcgagttg ctctcggcgg tgaagaagga tgccgcgctc aagcacatcc ccgtgctgat
     1501 ggtgaccgcc gaggcacgca aggaagacat cgtgcgtgcg gcgcaggatg gcgccgctgg
     1561 ctacatcgtc aagcccttca ccaaggccac cctcgaggag aaggtacaga agatcatgca
     1621 gaaactggcc gcaacggcgt gaggaccgca tcatgaacat gaacgaactc gccgcagccg
     1681 atctccagtc cgacatgacg tcgcatgctg cttcagccgc agcatcgacc gaggtgttcc
     1741 agcaactggg cgcgatcacc cgccagctgc acgacaccct cagtcagctc ggcgtgatgc
     1801 ccaagctgca gatcgccgcc gagggcctgc cggacgcgcg cagccgtctg aactacatcg
     1861 ccgagaagac cggtgcggca gccaacaagg tgctcaattc ggtcgactgt gccaaggccg
     1921 atcacctgct gatcacacag gaaacccgac gcatcgctgc ggcgatcgtc gccgatccgg
     1981 tgaaggcggt ggcctcgggc atgttgatga atttcgtcgg tgacgttgaa tccgcgacga
     2041 cgcgcatcga cggacacctg accgacatca tgatggcgca ggacttccat gacctgacgg
     2101 gtcaggtcgt cgccaaggtc gtcgcgctcg ccagcgagct cgaggacagc ctggtcaggc
     2161 tgctggtgca ggcggctccg ccagatcagg tgcagaaagt ggaacagcac ctgcagggtc
     2221 cggtggtcga cgccgaagga cgcaccgacg tggtcgccaa ccagggcgag gtcgacgacc
     2281 tgctggccag cctgggattc tgacgagccc cgctttcact cgtgagtcag tggcccggtc
     2341 gggctcctgg cgcgcgatgc ccgacgggcg cctggtccct gctcagccca cgccgccgcc
     2401 gaccaggccg ttgcggatcg catagaccgt catttccgag ttgttggcca ggcccagctt
     2461 ctccagcacc cgtgcacggt agacgctgac ggtcttcgga ctcagcatca gctcttccgc
     2521 gatgtcggac aggcgcttgc ccgaggcaat catcaccagc gtctgcaatt cgcggtcgga
     2581 caatttctga tgcgccgcct cgctcgtcgg cgtggtcagg ctctcgacca gcatctgtgc
     2641 gatctcggga gtcacgtact tgcggccctg cgccacggtt cgcaccgccg cgacgatcaa
     2701 cgccggatca ccccccttgt tgacgtagcc gaatgcccca gcacgcaagg cacggatggc
     2761 gtactggtct tccgggtaca tcgacacgac gagcacccgc agcgtcgagc cctcttcctt
     2821 caacgcatgc aacacgtcca acccgctgcg gcccggcagg ttgatgtcga gcaccagcac
     2881 atcgcaactg gcattgcgaa gcagactgcg caactcgccg tagtcgcccg cctcgccgac
     2941 cacctggatg tcgggtgcgt cggacagggt gtctcgaatc ccacgccgaa tcagcgcgtg
     3001 gtcgtcacag agcatcacgt tgatcatgtc ggaatcatag gtcgccccat ccggtgggat
     3061 cgtcggcatc cgccgggttg tccgggctgc cgcccggtgc gccgtccagg ggcaccgaga
     3121 gaatcagcgt ggtgccggtc ggtccgctgc tcaggtcgac ccagccaccc acggtgcggg
     3181 cacgctcgtg caggccccgg atgccgaacg agcgggcctt ggccagatcc tcggatgaca
     3241 ggccgcggcc gttgtcgctg atctcgagcg acagcacgcc gccggccatc gacagatcga
     3301 tggacacctg cgtcgcctgc gcgtgcttgc tgacgttggt gagtgcttcc tgagcggtgc
     3361 gataggcgac cagcggaacg ccgggtccga gcggcatcgg ctgctgcggc gcgcgcaagg
     3421 cgcaggcgat gccggtgcgt cgctcgaaac gcgcagtcat ccactgcagc gctgccagca
     3481 gcccctgttc gaggatggcg gggcgcaggt tgtgcatgat ccgctggctg gattcgatgg
     3541 ccgccgtgac ggtttccagc gcactctgca cccgggcctg cacctcgggc gaatcggagt
     3601 gacgtgcgat ccacgcgagg tcgaacttca gcgccgtcag cgagccacca acgtcgtcgt
     3661 ggatttcgcg tgcgatggcc gcgcgctcca tctcgatgct ggtctgcagg tgctgcgcca
     3721 gctcgtgcag gcgcagctgc gactcgccga gttgccggtc tgcacgctgg cgggcacggc
     3781 gcgtctcgct ggcctcgacg gcgtggagca atgccggtac cagccgcgcc aggttgttct
     3841 tcagcaggta atcgctggcc ccgttgcgca tggcctcgac cgcggtgtct tcgccgatct
     3901 cgcccgatac caggatgaaa ggcacgtcgc caccgcgggc catcagcagg tcgagcgcca
     3961 ccagccccga aaaaccgggc aggttgtagt cggaaatgat cgcgtcccac cggtcgtcga
     4021 gcgcgcgcag gaaatcggct tcgttgtcga cacgccgggg ttcgaccacc agtccacccc
     4081 gacgcaggtg cgcaagcatg agttcgtggt cgagctcgga gtcctcgagg tgcagcacac
     4141 gcagcggttg atcggcagac cgggacgcca tggcacgagt atgcagcctg ctccttcccg
     4201 gggctgccgg gagcggtcgc ctggtccagg ctgtgccact ggaggtgcca gcgggaagaa
     4261 cagccgtcgg aatagcagcg tgattgcgct tatgtattcg tgacagctgc cgaaaatcac
     4321 tcagacccaa agacgcctcg gacccgcaat tgcgtgccct tcggcactcg ctgccagctg
     4381 gtggcgacga caggcggtat cacagggagt acttatcaga tggaatacgc tggacaccag
     4441 gtcgagccga cgcaggtcgg gcaagcgccc gccccgctgc ttgtggccgc cgatcttcag
     4501 gaccacttga tgatggcgac caacgacctc gatcggctgc acacgctgct ggccgatgcg
     4561 tgcgacacgc tgatgcagcg cttctacaac gccaccgagc agatccacac gctgatggac
     4621 gagcacagct cggacggcga cgacaagcgc gactcggcgg cctaccagaa cctgatgcag
     4681 cagctgggag ccgccgttac cgcgctgcaa ttccaggaca tggcgtcaca gttgatcgtc
     4741 cataccaacc accgcctgcg cagctgcgta gacacgctgg cacaggaagc ctttggcgat
     4801 gacgacgagg atggacagag cgcagtcgcg gccccgccgc tgcgtcccaa cccggtcact
     4861 caatccgaga tggatgccgg ctcggtcgag ctgttctgaa gcctcgccga cacgccattc
     4921 agcccccgta tcaatcaacc actcacgttc cctccggaga cagcaatgca ctcaatcctt
     4981 gccgtggacg actcggcctc gatgcgccag atggtcacct tcacattgaa gagtgcgggc
     5041 tatcacgtcg tggaggccgt cgatggccaa gacgctctgg agaaatcggg gcagcaggat
     5101 ttcgacctgg tactgaccga tcagaacatg ccccgcatgg acggcatcaa cctgaccaag
     5161 aacctgcgca atcacccgaa gttcaaggcc actccgatcc tgatcctgac caccgaatcc
     5221 agtgatcaga tgaagcaggc cggccgcaat gccggcgcga ccggctggat ggtcaagccc
     5281 ttcgatcctg ccaagctgat cgaggtcatc cgcaaagtga ttcgctgaag cgcgccgacg
     5341 gcgcccgaga aaacacgcac gagacgacga ggggtgggtg atgagtgaca tgaacacaga
     5401 ccaatcaagc ggcgcaggca tcgacctgag ccagttctac caggtgttct tcgaagaagc
     5461 cggagagaac ctcgacagca tggagcagca gttgctggcc atcgacatcg aggcggccga
     5521 cgacgaggaa ctcaattcga tcttccgctg cgcccactcg gtgaagggtg gcgcggcgac
     5581 cttcggcttc gccgatgtcg ccgagctcac gcaccagatg gaaacgctgc tggaccggct
     5641 gcgccgccac gagctggaac cgacagctcc gatggtcgac gtgctgcttc agtcgggtga
     5701 tgcgctgcgc tcgcagctgg cacgtcatca aggtgcgggt ggcgacccgg tggacacgac
     5761 cgatctgctg gtgaagatcc gtgcgctggt tgccggtcag cccctgcccg cgtccgccgc
     5821 ggcagctgca cccgcaagcg ccgcacccaa atcagtgctg gcgcaggaac cagtcgcatc
     5881 ctccgctcct gctgccccgg ccaagtctgc cccggcagcg aagatggccc gcggcctgga
     5941 gttgcgagtc ggaccgctga ccgatccgaa ggacgccgac aacctcgtcg acctcttcaa
     6001 ggaaatcgtc gacctcggca cgatcgaacc gctcgacggc ggtcaggcga gcgagggcct
     6061 gcgccgcttc aaggtggtga ccaccagcag cgatgacgat ctgctcgacc tcttcacctt
     6121 ccacgtggca cgcgagaagg tggccctgct gccgctgacc tccggattcg gtttccacga
     6181 aggcgcgccc ggcgcacctc cggtcgagaa cccgcccgac ccgggcttcg gcttcttcga
     6241 ggatgcgcct ggcgcacccg gcagtgccag cccgacggcg gcctcgacct cgaacagccc
     6301 gactgcgccc atcgcgtctg aaagcgccag cgccgcagca cccgcacgcg caccggccaa
     6361 gaccgagaag gctccggccg cagcgctcga atcgtcgacg ctgcgcgtct cggtcgagaa
     6421 ggtcgatcaa ctcatcaatc tggtgggcga actggtcatc actcaggcca tgctcgcgca
     6481 gaacagcaag cagatcgaca ccgcgctcta ccagcagctc gctgctggcc tggccgacct
     6541 ggatcgcaac acacgggatc tgcaggagtc ggtgatgtcg atccggatga ttccgatgtc
     6601 gatggtgttc agccgcttcc cacgcatgct gcgggacctg gctgccaagc tcggcaagaa
     6661 ggtcgacttc gtgactcagg gggaagccac cgagctcgac aagggtctgg tcgagaagat
     6721 caccgatccg ctgacacacc tggtccgcaa cagctgtgac cacggcatcg aaatgccggc
     6781 cgaccgcctg gccaagggca agccggagac cggcacgatc acgctcgctg cttcgcacca
     6841 gggcggctcc atcgtcatcg aggttcgcga cgacggccgc ggcctgtcac gcgagaagtt
     6901 gatcaagaag gctcgcgagc gcggcatcga cgcgccggac tcgatgtccg acagcgaagt
     6961 ctggaacctg atcttcgcgc cgggcttctc gaccgccgac gtggtcaccg acgtgtcggg
     7021 ccgcggagtc ggcatggacg tggtgaagaa gaacatcacg tcgctgggcg gcaccgtcga
     7081 gatcgaatcg gccgagggct atggcatgcg cgtctcggtg cgcctgcca
//
LOCUS       DAOORV010000171         7088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_13030_length_7088_cov_9.216266, whole genome shotgun sequence.
ACCESSION   DAOORV010000171 DAOORV010000000
VERSION     DAOORV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_7088_cov_9.216266"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1375)
                     /locus_tag="PKV56_16025"
     CDS             complement(83..1375)
                     /locus_tag="PKV56_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOX69366.1"
                     /translation="MDIITAFLLIALLIAILASGFWIGLALLGVAVFSMEVFTSRPVG
                     DSMVFTIWGSTSSWTLTALPLFLWMGEILFRSRLTADLFTGLSPWLNRLPGRLLHANV
                     VGCGIFAAVSGSSAATCATIGKITLPELKKRGYPDAISMGSLAGAGTFGLLIPPSIIM
                     IVYAVATDVSIAKLFVAGVLPGIGLMAIFSGYLILWALRHPDQIPAADAAMSLGDKIR
                     AGRHLIPVLSLIVLVIGAIYTGLATATEAAALGVAGSLVLSRLEGSLTWAAFKDGLTA
                     ACRVYCMIGLILAGASFLTLAMGFIGLPREVAAFIGGLHLSPFWLLMLLMVFFIILGC
                     FLDGISMVVLTMAVLLPTVQSAGIDLIWFGIFVVLVVEMAQITPPVGFNLFVLQGLTG
                     RELPYLARTALPFFGLLVLAVLLIYVFPQVVLWLPSLM"
     gene            complement(1399..1911)
                     /locus_tag="PKV56_16030"
     CDS             complement(1399..1911)
                     /locus_tag="PKV56_16030"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOX69367.1"
                     /translation="MRHLLDRFYAALMALAALALLGTFVCVMLGIIDRQVGWGLRGLD
                     AYAGYGIAAALFLALPATLQKGEHIRVTLLLQRLPVAWRSGLEWWSLALGLALAGMLA
                     AYAWRLVWVSQTTNDISQGADATPLWLPQIAMALGCLGLFVALADAAWSRATGGSFEQ
                     ASGGEAARME"
     gene            complement(1935..2900)
                     /locus_tag="PKV56_16035"
     CDS             complement(1935..2900)
                     /locus_tag="PKV56_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOX69368.1"
                     /translation="MLKKLALILACAAACGGALAQTKWDLPSGYGPNTFQVQNLQWFA
                     DEVDKATAGKLKLTLHPGASLFKANEIKRAVQTGQTQIGEFILSGAANENALFGVDSI
                     PFLATTYPAAKKLDAASRPALDKLLASQGMKLLYTVPWPGQSLYSGKPVAALADLKGK
                     KMRAYNPATTRIAELAGAQPVTIQLAELPQALATGGVDAFLTSSASGVDSKLHEQVKY
                     FYDVNAWLPRNAVVVSTKAFDALDKPTQAALLKTAVAAGDRGWQVSEQRDGEYLKELA
                     AKGMTVDRSNTRLKTELKAIGDRMTAEWLKLAGADGQAVIDAYNK"
     gene            complement(2912..3727)
                     /locus_tag="PKV56_16040"
     CDS             complement(2912..3727)
                     /locus_tag="PKV56_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOX69369.1"
                     /translation="MLNHPYIHPAPGAGSSPVQVRAACRSAGFSGHTSGLAARHVQGN
                     VVILPKSLAADFQRYCERNPKPCPVLAMGEPGEVGLPALGVGIDMRCDLPRYRVWRHG
                     VLVAEPTHIADLWQDDFVSFALGCSFSFEQALLDDGIRLRHVEQGRNVAMYRTNLPTL
                     EAGPFRGPLVVSMRPLKPEDVDRAVQITARYPAVHGAPVHVGDPAQIGIPDLARPDFG
                     DAVDVLPGEVPVFWACGVTPQAALVQAAPPLAITHAPGCMLVTDLLNHQLAAA"
     gene            3871..4560
                     /locus_tag="PKV56_16045"
     CDS             3871..4560
                     /locus_tag="PKV56_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69370.1"
                     /translation="MTTTASSTTTTTQTLSERVAEQLRQKITQGEFAPGQRLSEQALS
                     ESLGISRNTLREVFRMLTKDGLVRHTPNRGVFVAIPTIASIIDIYRVRRLIECQALSQ
                     AYPHHPAKKKMREAVENALRCRAGRDWVGVGTANMAFHMAIVELADSERLNLLFAPVL
                     AELRLAFGLLRDAEFLHAPYVDMNVKILELAEAGEFAGAAAALTDYLVHSERIVLAVY
                     ARHISEAGWGS"
     assembly_gap    4635..4734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4911..6308)
                     /locus_tag="PKV56_16050"
     CDS             complement(4911..6308)
                     /locus_tag="PKV56_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HOX69371.1"
                     /translation="MITAREIDAATSKARASKKDVWLTDPGVRGRGRFTIRCTPSGAR
                     ICRYRYTCADGTRDILRVADYDPHGTAGMTLHEAREKAGELVRLAGAGNNVRVFLQER
                     DAAKAAAAEAALAATRNASRGSLAAMFRVYVGSLQGRESHYDAQSIFKLHVSGPFPKL
                     AARPVRQIKAAELRDVLARLIGAGKGRTAAKLRAFLRAAFSMAMRAGLDPTLPEAMTA
                     FDIQVNPADRLPSLAQFSKALNRALTLPELHAFWRRVEGAADSPARGALVACMYLGGQ
                     RPTQVLRLTPQMVDASGATLTLLDPKGRNRHANPRRHVLPIHADLVPALRARLAQAER
                     MESPLFSTNGRVPLRKETVALLVGEIAKAMQAASELERGPFSMRDLRRTAETHMAALG
                     VSSDVRAQIQSHGLGGIQARHYDRHDYMPEKRAALALWAQRFRNLPRAPRQTPPHTKS
                     RRVGERVAGQGVLAG"
     gene            complement(6376..6660)
                     /locus_tag="PKV56_16055"
     CDS             complement(6376..6660)
                     /locus_tag="PKV56_16055"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOX69372.1"
                     /translation="MFSSDFDYSIPSYQRPYAWTEVQAGELFSDLVDFFTKEKDDTYF
                     LGSIVLIKEEGKPPGEVIDGQQRLTTLTILLAALTTQFAGELRADFENYN"
BASE COUNT         1207 a         2338 c         2303 g         1140 t
ORIGIN      
        1 gcgtcgtcgt cgcccatgcg ccaggccccg aggctctcgc cgaggtcgct gttcaagtcc
       61 atggcgtact ttcggtgcgt ggctacatca ggctgggcag ccacagcaca acttgcggaa
      121 acacatagat cagcagcacc gccagcacca gcaggccgaa gaagggcagc gcggtgcgcg
      181 ccagataggg cagctcacgg cctgtcagcc cctgcagcac aaacaggttg aagccgaccg
      241 ggggcgtgat ctgcgccatc tccaccacca gcaccacgaa gatgccgaac cagatcaggt
      301 cgatgccggc cgactgcacc gtgggcagca gcacggccat ggtcagcacc accatgctga
      361 tgccgtcgag aaagcagccc aggatgatga agaacaccat cagcagcatc agcagccaga
      421 acggcgacag gtgcaagccg ccgataaagg ccgccacctc gcgcggcagg ccgatgaagc
      481 ccatggccag cgtcaggaag gacgcaccag ccaggatcag gccgatcatg cagtacaccc
      541 ggcaggccgc tgtcagcccg tccttgaagg cggcccaggt cagcgagcct tcaagccggc
      601 tcagcaccag cgagccggcc acgcccagcg cagcggcctc ggtggccgtg gccaggccgg
      661 tgtagatcgc gccaatcacc agcacgatca gcgacagcac cgggatcagg tgccgcccgg
      721 cgcggatctt gtcgcccagg ctcatcgccg catcggcggc cggaatctgg tcaggatggc
      781 gcagcgccca gaggatcaga tagccgctga agatggccat cagcccgatg cccggcagca
      841 cgccggccac aaacagcttg gcgatcgaga catcggtggc caccgcgtag acgatcatga
      901 tgatcgacgg cgggatcagc aggccgaagg tgcccgcgcc ggccagtgac cccatgctga
      961 tggcatcggg gtagccgcgc ttcttgagct cgggcagcgt gatcttgccg atggtggcgc
     1021 aggtggccgc actgctgccg gacaccgccg cgaagatgcc gcagcccacc acattggcat
     1081 gcagcaatcg ccccggcagc cggttgagcc agggtgacag cccggtgaac aggtcggccg
     1141 tgagccggct gcggaacagg atctcgccca tccacaggaa cagcggcaat gcggtcagcg
     1201 tccagctgct ggtgctgccc cagatggtga acaccatgct gtcgcccacc gggcggctgg
     1261 tgaacacctc catcgagaac acggccaccc ccagcagggc caggccgatc cagaagcccg
     1321 atgccaggat ggcgatcaac agggcgatca acaggaaagc ggtgatgatg tccatggtgt
     1381 ggccgtgccg tcctgttgtt attccatgcg ggcggcttca ccgccgctgg cttgctcgaa
     1441 gctgccgccc gtcgcccgcg accaggccgc atcggccagg gccacgaaca ggcccaggca
     1501 gcccagcgcc atcgcgatct gcggcagcca cagcggcgtg gcgtcagcgc cctgcgagat
     1561 gtcgttggtg gtctgcgaca cccagaccag gcgccaggca taggccgcca gcatgccggc
     1621 gagcgccagc cccagggcca ggctccacca ttccaggccg ctgcgccagg ccaccggcag
     1681 ccgctgcagc agcagggtga cacggatgtg ctcgcccttc tgcagcgtgg cgggcagcgc
     1741 cagaaacagc gcggcggcaa tgccgtagcc ggcataagca tcgagcccac gcaggcccca
     1801 gccgacctgg cggtcgatga tgcccagcat cacgcagacg aaggtgccca gcaaagccag
     1861 ggccgccagc gccatcagcg cggcataaaa ccggtcgaga aggtggcgca tggtgcggtg
     1921 gcccggtgat tggcttattt gttgtaggcg tcgatgacgg cctggccatc agcgccggcc
     1981 agcttgagcc attcggcggt catgcggtcc ccgatggcct tgagctccgt cttcaacctg
     2041 gtgttgctgc ggtccaccgt catgcccttg gccgccagct ccttgaggta ttcaccgtca
     2101 cgctgctcgc tcacctgcca gccgcggtca ccggcggcga cggcggtctt cagcagcgca
     2161 gcctgtgtgg gcttgtccag cgcgtcgaaa gccttggtgc tgacgaccac tgcattgcgc
     2221 ggcaaccagg cgttcacatc gtagaagtac ttcacctgct catgcagctt gctgtccaca
     2281 ccgctggcgc tgctggtgag aaaggcatcc acaccaccgg tggccagggc ctgcggcagc
     2341 tcggccagct ggatggtgac cggctgcgcg ccggccagct cggcaatgcg tgtcgtcgcc
     2401 gggttgtagg cgcgcatctt cttgcccttc aggtcggcca gcgccgccac cggcttgccg
     2461 ctgtagagcg actggccggg ccagggcacg gtgtagagca gcttcatgcc ctggctggcc
     2521 agcagcttgt ccagggccgg gcggctggcg gcatcgagct tcttcgcggc cgggtaggtg
     2581 gtggccagga aggggatgga gtcgacgccg aacagggcgt tctcgttggc cgcgccggag
     2641 aggatgaact cgccgatctg ggtctggccg gtctgcaccg cccgcttgat ttcgttggcc
     2701 ttgaacagcg acgcgccggg gtgcagcgtg agcttcaact tgcccgccgt ggccttgtcg
     2761 acctcgtcgg caaaccactg caggttctgc acctggaagg tgttggggcc atagcccgag
     2821 ggcaggtccc atttggtctg cgccagcgcg ccgccgcagg cggcagcgca ggccaggatc
     2881 agtgcgagtt tcttcagcat cagggttctc ctcaggcagc agccagttgg tggttcagca
     2941 ggtccgtcac cagcatgcag ccgggtgcat gggtgatggc caggggcggt gcggcttgca
     3001 ccagggcggc ctgcggcgtg acgccgcaag cccagaacac gggcacctcg cccggcagca
     3061 catcgactgc gtcgccgaag tcgggccggg ccagatcggg gatgccgatc tgcgccgggt
     3121 cacccacatg gaccggcgca ccatgcaccg ccgggtagcg ggcggtgatc tgcaccgcgc
     3181 ggtcaacatc ctcgggcttc aacggccgca tgctgaccac cagcggcccg cggaacgggc
     3241 ccgcctccaa cgtgggcaga ttggtgcggt acatggccac gttgcggccc tgctcgacat
     3301 ggcgcagccg gatgccgtcg tccagcagcg cctgctcgaa gctgaagctg cagcccagcg
     3361 caaagctcac gaagtcgtcc tgccaaaggt cggcgatgtg ggtgggttcg gcgaccagca
     3421 cgccgtggcg ccagacccgg tagcgcggca ggtcgcagcg catgtcgatg cccacaccca
     3481 gcgccggcag gcccacctcg cccggctcac ccatggccag caccgggcac ggcttggggt
     3541 tgcgctcgca ataacgctga aaatcagcgg ccagtgactt cggcaggatg accacattgc
     3601 cctgcacatg ccgcgcggcc agcccgctgg tgtgaccgct gaagccagcg ctgcggcagg
     3661 ctgcacgcac ctgcaccggg ctgctgccgg caccgggcgc tggatgaatg tatggatgat
     3721 tgagcattaa ttgttcaaca attaatgaat gttagcccta cattcaccgc cgtttcatca
     3781 gtgaaaaccc aagtcctgct gagcaatcac cgccgccgtg tggtgagatc ggcaacaacc
     3841 ccggcggcgc cacccaacga tcccaaccgc atgaccacca ctgcgtcctc caccaccacg
     3901 accacccaaa cgctgagcga gcgggtggcc gaacaactgc ggcagaagat cacgcagggc
     3961 gagtttgcac ccggccagcg cctgtcggaa caggccctga gcgagagtct gggcatctcg
     4021 cgcaacacgc tgcgcgaggt gttccgcatg ctcaccaagg acggcctggt caggcacacg
     4081 ccgaatcgag gtgtgttcgt ggcgattccc accatcgcgt cgatcatcga catctaccgg
     4141 gtccgccggt tgatcgaatg ccaggccctg tcgcaggcgt atccgcacca tccggcgaag
     4201 aagaagatgc gcgaagcggt ggaaaacgca ctgcgctgtc gggcgggccg cgactgggtg
     4261 ggcgtgggca ccgccaacat ggccttccat atggcgatcg tcgaactggc cgacagcgag
     4321 cggctcaacc tgctgtttgc gccggtgctg gccgaactgc ggctggcgtt cggcctgctg
     4381 cgcgacgccg agttcctgca tgcgccgtac gtcgacatga acgtgaagat cctggaactg
     4441 gccgaggccg gcgaattcgc cggtgccgcc gcggcgctca ccgactactt ggtccattcc
     4501 gagcgcatcg tgctggctgt ttacgcaagg cacatttccg aggccggttg gggcagctga
     4561 ctccggttcg aggaggcagt aatgacattc agccgctgct cgttggcaac gtctcgcgcg
     4621 cgtgcggttc ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacgtc
     4741 tcgcgcgcgt gcggttcctg gactggcagt gcggattccg cccgagtgcc tgcggtggac
     4801 gtgccagtgg cgtccggggc cgagcagctg ctggcaacgg cgtccgccgt gctaaacgcc
     4861 ccacgctcga gctcgccagc ggccagcatg gtcttctcga catcctctgc ctatccggcc
     4921 aacactcctt gccctgcgac tcgctcccca actcgccggg acttcgtatg gggaggagtt
     4981 tgtcgaggcg cccgtggcag attgcgaaaa cgctgtgccc acaacgcaag cgccgcgcgc
     5041 ttctccggca tgtagtcatg gcggtcgtag tggcgggcct ggatgccacc aagcccgtgc
     5101 gattgaatct gcgcgcgcac gtcacttgag actccgagcg ccgccatgtg cgtttcagcc
     5161 gtgcgccgca gatcccgcat cgaaaacggc ccgcgctcaa gctcgctcgc ggcctgcatc
     5221 gccttcgcga tttctccgac cagcagcgcc accgtctcct tgcgcagcgg cacgcgcccg
     5281 ttcgtgctga agagcggcga ctccatgcgt tcggcctgcg ccaggcgcgc gcgtagtgcg
     5341 ggcaccaggt ccgcgtggat gggcagcaca tgccgacgcg ggttggcatg gcgattccgc
     5401 cccttggggt ccagcagggt cagcgtggcg ccagaagcgt ccaccatttg cggcgtcagg
     5461 cgcagcacct gtgtcggtcg ttgaccaccg agatacatgc aagctaccaa cgcacccctt
     5521 gcgggactgt cggccgcgcc ctcgacgcgg cgccagaacg catgaagctc gggaagcgtc
     5581 agcgcgcggt tcagtgcctt cgagaactga gccaggctcg gcagccggtc ggcggggttg
     5641 acctgaatgt cgaaggccgt catcgcctcg ggcagcgtcg ggtccaggcc cgcgcgcatg
     5701 gccatgctga aggcagcgcg caggaaggct cgcagcttgg cggcggtccg ccccttgccg
     5761 gccccgatca gccgtgcgag cacgtcgcgc agttccgcgg ccttgatctg tcggaccggg
     5821 cgggcggcca gcttcgggaa cgggccactg acgtgcaact tgaagatcga ctgcgcgtcg
     5881 tagtgcgact cccgaccctg caggctgccg acgtagacgc ggaacatcgc cgcaagactg
     5941 ccgcgcgatg cgttgcgcgt cgcggccagc gccgcttcag cagctgcggc cttcgccgca
     6001 tcgcgctcct gcagaaagac tcgcacgttg ttgccggcgc cggcaagccg caccaactcg
     6061 ccggccttct cgcgcgcttc gtgcaaggtc atgcccgccg tgccgtgggg gtcgtagtcg
     6121 gccactctca ggatgtcgcg cgtgccatcg gcgcacgtgt agcggtacct acagatccgt
     6181 gcgccggacg gcgtgcagcg aatggtgaag cgtccccggc cgcgcacacc ggggtcggtc
     6241 agccacacgt ccttcttgct cgccctggcc ttgctggtgg ccgcatcgat ctcgcgtgcc
     6301 gtgatcacgc ctgcgcgcgc acccgcgttt cgcgttgtcc ggagtgcagt cgctgccgca
     6361 gtgtctgttg ccgtttcagt tgtagttctc gaagtcggcg cgaagctcgc cggcgaactg
     6421 cgtggtcagc gccgccagca gaatggtcag ggtggtcagt cgttgttgcc cgtcgatgac
     6481 ctccccaggc ggcttgcctt cttccttgat gagcacgatg ctgcccagga agtaggtgtc
     6541 gtccttctcc ttggtgaaga agtcgaccaa gtcggagaac agctctcccg cctgcacctc
     6601 ggtccatgcg tacggccgct ggtaggaagg gatggagtag tcaaagtcgg agctgaaaat
     6661 cttggcgagc ggaaactcgg ctccgctgat cttcttggtc ctgcaaactc cctagccttg
     6721 ctttctgcaa ccattgttca atcatggtga gggacaggag cgtcgctgga gaggaacggc
     6781 gcgcgatctg gcaggcagaa tcgccaaggc gcatgcgggc atccgcgagc gccacttcgg
     6841 gcgattgcgg gtgccctcgc tagttgatgc tgcagacctt cgatcgcgcg ctcaggcgct
     6901 tgcctcccgg cacagtcctt ggcgatcaag ctaaacatct gtatacggct cgctgtggga
     6961 ctctccgcaa gggtgcctgt gaagccgcgg cctccgggcc aactgcagag gcgttcccgg
     7021 actgattgcg gagacgaaaa gccgctaagc tgttgatttg taggacttct gctgagaact
     7081 tgaaaacc
//
LOCUS       DAOORV010000172         7037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_13142_length_7037_cov_6.098396, whole genome shotgun sequence.
ACCESSION   DAOORV010000172 DAOORV010000000
VERSION     DAOORV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13142_length_7037_cov_6.098396"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..1471)
                     /locus_tag="PKV56_16060"
     CDS             complement(263..1471)
                     /locus_tag="PKV56_16060"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOX69373.1"
                     /translation="MLVWEEEIKSSMPPMASVATTRIPEEIQSRSSDAGISGMDLSRS
                     MVSSVSPRAGTIAPAQPVENRRVVAADKRIINGQTDVNQLVPFKYKWAWEKYLATCAN
                     HWMPQEVNMSRDIATWKDPNGLSDDERRIIMRNLGFFVTADSLAANNIVLGTYRHITA
                     PECRQFLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYNEVASIRDKDQFLIPFIEA
                     VMDPHFNTGTLENDQKLLKSLIVFACLMEGLFFYVGFTQILALGRQNKMTGASTQYQY
                     ILRDESMHCNFGIDLINQIKLENPLLWTAEFKAEIKALFVRAVELEYRYAEDTMPRGV
                     LGMNASMFKGYLRYIANRRATQIGLEALFPNEENPFPWMSEMIDLKKESNFFEVRVTE
                     YQSGGALSWD"
     gene            complement(1617..4517)
                     /locus_tag="PKV56_16065"
     CDS             complement(1617..4517)
                     /locus_tag="PKV56_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOX69374.1"
                     /translation="MQALQSTPGAYPYVAERANTAAAAPASAFDAFHIIRRNGAVVPF
                     EPNKIAVALMKAFLAVHGTQGAASASVRETVDGLTESVVRALLRSRPGGGTFHIEDIQ
                     DQVELGLMRGGHHEVARAYVLYRERRAQERARQVQPVAVAESTLTVLDRGQRVPFDSV
                     HLQTIVQSACEGLGADVRAEPILLETKRNLYDGVPIDEVHKASILAARTLIEKDPGYT
                     RATARLLLHTIRREILGEEVLQSDMRTRYADYFPGFIKKGVHAELLDDKLLQFDLARL
                     GAALNADRDMQFDYLGLQTLYDRYFLHIDEARIELPQAFFMRVAMGLALNEIDREARA
                     IEFYEVLSTFDFMSSTPTLFNAGTRRSQLSSCYLTTVADDLDGIYEALKENALLSKFA
                     GGLGNDWTNVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLESW
                     HLDLEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMEGSDWTLFSPSTCPDLHDK
                     FGKEFEVAYTAYEARAAAGEIKLHKKVAARDLWRKMLTMLFETGHPWITFKDACNVRS
                     PQQHVGVVHSSNLCTEITLNTSASEIAVCNLGSVNLAHHLVDGPSGRQIDHAKLKKTV
                     STAMRMLDNVIDINYYAVKKARDSNLRHRPVGLGIMGFQDALHELRVPYASDAAVDFA
                     DCSMEAVCYHAYWASTELAAERGRYSSYRGSLWDRGILPIDSLDLLAEQRGGYVDVDR
                     SSTLDWSALRGRIAEHGMRNSNCVAIAPTATISNIIGVSASIEPSFGNLSVKSNLSGE
                     FTVLNEYLVRDLKKLGLWDDVMVMDLKHFDGSLRRIDRVPEDLKSLYATAFEIETQWL
                     VEAAARRQKWIDQAQSLNIYMAGVSGKKLDETYKLAWTRGLKTTYYLRTLGATHAEKS
                     TVKAGQLNAVSSGSSGGVDAAAAAPQAWSTSAEPASDIKYCAIDNPDCEACQ"
     gene            complement(5011..5616)
                     /gene="ampD"
                     /locus_tag="PKV56_16070"
     CDS             complement(5011..5616)
                     /gene="ampD"
                     /locus_tag="PKV56_16070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOX69375.1"
                     /translation="MASVALAEVDGDWHRGWWARARHCPSPNFGPRPTGVVPTLAVVH
                     SISLPPGTYGGAQVEQFFLNQLDVSAHPYFERLTGLRVSAHFFIRRDGACIQFVSCLE
                     RAWHAGASIWRGRSNCNDFSIGIELEGIEGAGFDDAQYARLCELLTSFVGPAGSIDVA
                     GHEHVSTGRKGDPGCGFDWGRTVAALGWPARCFPEGMVTVT"
     gene            complement(5594..>7037)
                     /locus_tag="PKV56_16075"
     CDS             complement(5594..>7037)
                     /locus_tag="PKV56_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX69376.1"
                     /translation="RREKAIVITAYGSAENAVEALKGGAYDYLTKPVDLKQFRAVVAS
                     ALGRGPSTAPSTLPSSLPTERSLQSVPPTADAAVARLAGDLSGARVEPVVSREAHSPL
                     RPPPQMATAAASPSVALNRMAGQSLAMKQVRALVDKVARSMAPVLVNGESGTGKELVA
                     RAIHEVSPRSAQPFIAVNCSAIPEHLLEAEFFGYRRGAFTGANEDREGFFQAADGGTL
                     FLDEIGDLPLSMQSKLLRAVQERSVRPVGAVTELPVNVRLLSATHKDLGAEVQAGRFR
                     QDLYYRLNVIQIRVPPLRERLEDLASIGERVLERIARDAGVWPPPRLTHAALQHLAGY
                     PFPGNVRELENLLHRAVALSGGETIDVADLGLPTDPQGELLHGDADRSATPSDAVSSM
                     LNAAARVEAAQSIPQAQVPPVGLPSDLAAYLDDIERDMLLRALEQHRFNRTAAGASLG
                     LSLRQMRYRMARLHVMAGADPHPDGERGAG"
BASE COUNT         1363 a         2206 c         2134 g         1334 t
ORIGIN      
        1 cgccggggcc ttcttggccg gggcggcctt cttcgcagtt gccattacat ttctccttga
       61 tcaagttaaa gaagcactgt cctgttcctg caaaaagccg gttccagaca gctattcatc
      121 acccgcaaac tgcccgtgaa gggcgccccg gtgcgatgaa tcgggtatca aaccgaggga
      181 aacgcttctg cgtcgtcacc actcgatttg cagatcttga tctgttcaca ttcatctcgc
      241 gcgatatcgc gctcgatcac tttcaatccc acgagagcgc accaccggac tggtattcgg
      301 tcacacgcac ctcgaagaag ttgctttctt tcttcaggtc gatcatttcg ctcatccagg
      361 gaaacgggtt ttcctcgttc gggaaaagcg cttccagccc gatttgagtg gcgcgccggt
      421 tggcgatgta acgcagatat cccttgaaca tggatgcgtt catccccaaa accccgcggg
      481 gcatcgtgtc ctcagcatag cgatattcaa gttccactgc tcgcacgaaa agagccttga
      541 tttcagcctt gaattcagca gtccagagca gcgggttctc cagtttgatc tggttgatca
      601 ggtcgatgcc gaaattgcaa tgcatcgatt cgtctcgaag gatgtactgg tattgggtag
      661 aggcaccggt cattttgttc tggcgaccca gcgcaaggat ctgggtgaac cccacgtaga
      721 aaaacagacc ctccatcagg caggcaaaaa caatcagcga tttcaggagt ttctgatcgt
      781 tctccagcgt gccagtgttg aagtgcggat ccatcaccgc ctcgatgaac ggaatcagga
      841 actggtcctt gtcccggatc gatgcgacct cgttgtaggc gttgaatatt tcgccttcat
      901 ccagacccag cgattcgacg atgtactgat aggcgtgggt atgaatggcc tcttcaaagg
      961 cctgacgaag caaaaactgc cgacattccg gagcggtgat atgccggtag gttcccagaa
     1021 cgatgttgtt tgccgccaga gaatccgcag tcacgaagaa cccgaggtta cgcatgatga
     1081 tgcgacgctc gtcatcgctc agtccgttag gatctttcca cgtggcgata tcgcgcgaca
     1141 tgttgacctc ctgcggcatc cagtggtttg cgcaggtagc gagatatttc tcccaagccc
     1201 atttgtactt gaatgggacc agttgattga catcggtctg gccgttgatg atgcgtttat
     1261 ccgcagccac cacccgccgg ttctcgacgg gctgggccgg ggcaattgtc ccggcgcgag
     1321 gggaaacact ggaaaccatc gatcgagaca aatccatgcc ggaaatgccg gcatccgatg
     1381 agcggctctg aatttcctca ggaatccgtg tcgtcgcgac ggacgccatg ggcggcatcg
     1441 atgatttaat ttcttcttcc caaaccaaca tggcttactt ccagtagata ttgattatcc
     1501 aatcgatggg acaaaacaca aagcgttagt tgcctcatac cgcttggctc ggatcgcatt
     1561 tgttgttccg tcgcgtcgca actgacggtt tcaagcaccg aatcgcaatc gagcgctcac
     1621 tggcaggctt cgcaatcggg gttgtcgatc gcgcagtact tgatatcgct tgcgggctcc
     1681 gcgctggttg accaggcctg cggggccgct gcggccgcat ccacacctcc ggacgacccg
     1741 ctgctcaccg cattgagttg ccccgccttc accgtggact tctcggcatg cgtggcaccc
     1801 agggtgcgca ggtagtaggt ggtcttcagg ccacgggtcc aggcgagctt gtaggtctcg
     1861 tcaagcttct tgcccgacac gccggccatg tagatgttca atgactgagc ttgatcaatc
     1921 cacttctgcc ggcgagcggc ggcttccacc aaccattggg tctcgatctc gaatgcggtg
     1981 gcgtaaagcg acttcagatc ctctggaacg cgatcgatgc ggcgcagcga tccgtcgaaa
     2041 tgcttgaggt ccatgaccat cacgtcgtcc cacaggccca gtttcttcag gtctcgcacc
     2101 aggtactcgt tgagtacggt gaattcaccc gacagattcg acttgacgga gaggttgccg
     2161 aaagacggct cgatcgacgc actgacaccg atgatgttgg aaatcgtcgc ggtcggcgca
     2221 atcgcaacgc aattggaatt gcgcatgccg tgctcggcga tgcggccgcg cagagcgctc
     2281 cagtcgagcg tgctgcttcg atcgacatcg acgtaaccac cacgctgctc ggcaagcagg
     2341 tcgagcgagt cgatgggcag gataccccga tcccagagcg agccgcgata gctggaatag
     2401 cgcccgcgct ccgccgcgag ttcagtcgat gcccagtagg cgtggtagca caccgcttcc
     2461 atcgagcaat cggcaaaatc gaccgctgca tccgacgcgt aaggcacgcg cagctcatgc
     2521 aaggcatcct ggaaccccat gatgccaagc ccgaccgggc gatgacgaag attcgagtcg
     2581 cgggccttct tcacggcgta gtagttgatg tcgatcacgt tgtcgagcat gcgcatcgcg
     2641 gtggacacgg tcttcttcag cttcgcgtgg tcgatctgcc tgccgctggg gccgtcgacg
     2701 aggtggtgag ccaaattcac ggaaccgagg ttgcacaccg ctatctctga tgcgctggtg
     2761 ttaagcgtga tctcggtgca caggttgctg gagtgcacga cgccgacatg ctgctgcggc
     2821 gagcgcacat tgcatgcgtc cttgaaggtg atccagggat gcccggtctc gaacagcatc
     2881 gtcagcatct ttcgccacag gtcccgagcc gccaccttct tgtgcagctt gatctcgcct
     2941 gcagctgcgc gcgcttcgta agcggtgtag gcaacctcga attccttgcc gaacttgtcg
     3001 tgcagatcgg gacaggtact cggcgagaaa agcgtccagt cgctgccctc catcacacgc
     3061 ttcatgaaca ggtcggggat ccagttcgcg gtgttcatgt cgtgggtgcg acgacggtcg
     3121 tccccggtgt tcttgcgcag ctcgaggaac tcctcgaggt cgaggtgcca gctctccagg
     3181 taggcacaca ccgcaccctt gcgctttccg ccctgattga cggcgactgc ggtgtcgttg
     3241 acgaccttga ggaagggaac gaccccctga ctcttgccgt tggtaccctt gatgtggctg
     3301 ccgagcgcac gcacattggt ccagtcgttg ccgaggccac cggcgaactt ggacagcagc
     3361 gcgttctcct tcagcgcctc atagatgccg tcgaggtcgt cggccaccgt cgtcaggtag
     3421 caactcgaca actgcgagcg acgggtgccg gcattgaaga gagtcggcgt cgaactcatg
     3481 aaatcgaacg tcgagagcac ttcgtagaac tcgatggctc gcgcttcgcg atcgatctcg
     3541 ttgagcgcca gtcccatcgc cacgcgcatg aagaaggctt gaggcaactc gatgcgtgcc
     3601 tcgtcgatgt gcaggaagta gcggtcgtac agcgtctgca ggccgaggta gtcgaactgc
     3661 atgtcacgat cggcattcaa cgcagcgccc agtcgggcca ggtcgaactg cagcagcttg
     3721 tcgtcgagca gttcggcatg cacccccttc ttgatgaatc ccgggaagta atccgcatag
     3781 cgtgtgcgca tgtcggactg cagcacctcc tcgccaagga tctcgcgtcg gatcgtgtgc
     3841 agcaggagtc gtgcggtggc gcgggtgtaa cccgggtcct tttcgatcag cgtgcgcgcg
     3901 gcaaggatgg aagccttgtg cacttcatcg atcggcacgc cgtcgtaaag gttgcgcttg
     3961 gtctccagca ggatcggctc ggcacgcaca tcggcgccca gcccttcgca ggccgactgg
     4021 acgatggtct gcagatggac cgaatcgaac ggcacgcgct gcccccgatc caggacggtc
     4081 agcgtcgatt cggcgacggc aactggctgc acctgcctgg cacgctcctg ggcacgcctc
     4141 tcacggtaga gcacgtaggc acgggcgact tcgtggtggc cgccacgcat caacccgagt
     4201 tcgacctgat cctggatgtc ctcgatgtgg aaggtgccgc cgcccggccg cgaacgcagc
     4261 agcgcgcgaa ccaccgattc agtcaaccca tccaccgtct ctcgcacgct cgccgatgcc
     4321 gcaccctgcg tgccgtgcac ggcaaggaag gccttcatca gggccactgc gatcttgttc
     4381 ggctcgaacg gcaccaccgc accgttgcgg cggatgatgt gaaacgcgtc gaaggccgac
     4441 gcgggtgcag cggcagcggt attcgcccgc tcggcaacat aagggtaggc gcccggcgtg
     4501 gattggagtg cttgcatgaa tggcctttgg tttggagagg ggtcggggac cgcactgctc
     4561 gggtgatgtc gatgccggcg ctcagggccg ccggcaggcc cgacaaaatt caggtcaacg
     4621 gacggacccc gcgccaccga aactgcacga tcgagtgcaa ccagccgagg ctgtcggtgc
     4681 gttggaaggc gtaggagcga ctgacaagac ttggcgcatc ggcacactat atctggggtc
     4741 ccgggccgct tcaagcacta ccgctagtgt cttgggcaca aactgaccac tccacgatcg
     4801 cgccaaacga tgccacctgc aatgcagttc gtcgggtgct gatcggccgc cgccctgcac
     4861 acccgcgagc gcgttgcatc gcacttcacg aagccgccaa atcattggcc tgcggcctga
     4921 actttcacgg cggaacgcac ggacactcag gtcaggcttc ggcgaagtcg caactcggcc
     4981 cagggagccg gtgcaacgtc accgcctcac tcaggttacg gtgaccatgc cctcgggaaa
     5041 acaccgtgcg ggccagccca gtgcagcaac cgtccgaccc caatcgaatc cgcagccagg
     5101 gtcgcccttg cggccggtgg agacgtgctc gtggccggca acatcgatgg agcctgcggg
     5161 accgacgaat gatgtcagca gttcgcaaag ccgtgcgtac tgcgcgtcgt cgaagcccgc
     5221 cccctcaatg ccttcgagtt cgatgccgat cgaaaaatcg ttgcagttcg aacgcccgcg
     5281 ccagatcgaa gcacccgcat gccaggcgcg ctcgaggcac gacacgaact ggatgcacgc
     5341 accgtcgcgg cggatgaaga aatgggccga cacgcgcagg cccgtcagac gctcgaaata
     5401 ggggtgcgcc gagacatcga gctgattgag gaagaactgc tcgacctgcg caccgccgta
     5461 ggtaccagga ggcaggctga tcgagtgcac cacggccagc gttggaacaa ctccggtcgg
     5521 gcgcggcccg aaattcggcg atgggcaatg tcgcgccctc gcccaccagc cccgatgcca
     5581 gtcgccgtcg acctcagcca gcgccacgct cgccatcggg gtgcgggtcg gcgccagcca
     5641 tgacatgaag ccgtgccatg cggtagcgca tctgccgcag cgacagtccg agactggctc
     5701 cggcggccgt gcggttgaat cggtgctgct ccagagcgcg caacagcatg tcgcgctcga
     5761 tgtcgtcgag gtaggccgcc agatcgctgg gcagacccac cggcggaacc tgcgcctgcg
     5821 gaatgctctg cgcagcctcg acacgcgccg ccgcattcag catcgagctc acggcatcgg
     5881 acggcgtggc gctacggtct gcatcgccat ggagcaactc gccctgcgga tcggtcggca
     5941 ggcccagatc tgcgacatcg atcgtctcac cgcccgagag agccaccgcg cgatgcagca
     6001 gattctcgag ctcacgaacg ttgccgggaa aggggtaccc ggcgagatgc tgcaacgccg
     6061 catgcgtcag cctcggcggc ggccagacac cagcgtcgcg ggcgatccgt tcgagcaccc
     6121 gctcgccgat gctcgcgaga tcctcgagtc gctcgcgcaa cggcggaact cgaatctgga
     6181 tcacgttgag ccggtaatac aggtcctgcc gaaatcggcc ggcctgcacc tcggcgccga
     6241 ggtccttgtg cgtggcactc agcagacgca cattgacagg cagttcggtg acggccccga
     6301 ctggacgcac cgatcgctcc tgaaccgcac gcagcagctt cgactgcatc gacaacggaa
     6361 gatctccgat ctcgtcaagg aacaaggtgc ccccgtccgc cgcctgaaag aagccctccc
     6421 ggtcttcgtt ggccccggtg aatgcaccac gccgataacc aaagaactcg gcctcgagca
     6481 ggtgctcggg aatggcgctg cagttcactg cgatgaatgg ctgcgcactg cgcgggctga
     6541 cctcgtggat cgctcgcgcg accagttcct tgccggtgcc ggactctcca ttgacgagca
     6601 ccggagccat gctgcgagcg accttgtcga ccagcgcacg cacctgcttc attgcgagcg
     6661 actgcccggc catccggttg agcgccacgc tgggcgacgc cgcggcagtc gccatctgcg
     6721 ggggtggtcg caggggagag tgcgcctccc gcgagacaac gggttcgacc cgtgccccag
     6781 acaggtcacc ggccaaacga gcgactgctg cgtccgccgt cggagggacc gactgcagcg
     6841 agcgctcggt cggcaagctg gagggcagcg tgctgggcgc ggtcgacggc cccctgccga
     6901 gcgctgacgc gaccaccgca cgaaactgtt tcagatcgac cggcttggtc aggtaatcgt
     6961 aggcgcctcc cttcagcgcc tccacggcgt tctcggcaga cccgtaggcg gtgatgacga
     7021 tggccttttc acgccgg
//
LOCUS       DAOORV010000173         6924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_13425_length_6924_cov_7.357257, whole genome shotgun sequence.
ACCESSION   DAOORV010000173 DAOORV010000000
VERSION     DAOORV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13425_length_6924_cov_7.357257"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKV56_16080"
     CDS             <1..257
                     /locus_tag="PKV56_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HOX69377.1"
                     /translation="IEKLIKRQIPSEVVPGFEPPSGEKAEPIVLGRMTIGVGGTRRNA
                     GGGFGGGRPGGGGGGGGGGGGGRSGGGGYGGGGGGNRGGY"
     gene            complement(370..1155)
                     /locus_tag="PKV56_16085"
     CDS             complement(370..1155)
                     /locus_tag="PKV56_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX69378.1"
                     /translation="MNAPLVYITGASSGIGLALAERFAQAGYRLALVARRAPEIAAWS
                     RSRGLGPSACVVYAADVRDIDSITGAGRACIAGQGLPDVVIANAGISIGMDTAEFDDM
                     EVMRATFETNNLGLAATFQPFVRAFCERRSGTLVGIASVAGIRGLPGHGAYCSSKAAV
                     ISYCESLRGECRPFGVKVVTLSPGYIDTPLTRDNRYPMPFLMSPAAFAERAVDAIEQG
                     ASYRVIPWQMGIVAGFLRVLPNAWLDRVLAGQPRKHRKPKSIG"
     gene            complement(1192..1950)
                     /gene="lptC"
                     /locus_tag="PKV56_16090"
     CDS             complement(1192..1950)
                     /gene="lptC"
                     /locus_tag="PKV56_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOX69379.1"
                     /translation="MTFADSTLLPVDEPAERRFVLPWYQRVLSGLTAYLPLVLMALLA
                     LATWWLVKNTPLYQGEKPAVAPRHIPDYTMRQFTVQRFSADGRLRAQIEGDELRHYPD
                     TDTLEMDNARVRSIGIDGHVTLATAKSALSNGDGTELQLTGQAHVVREAFASASGVEE
                     AVDFRSEFLHAFLRTEQVRSHLPVTITRGSTQIQGASMHYDNLSRVAQFNGRVRASFL
                     PSSQTPGAPLPATDRSVDRSPGVSNSRPASAAQR"
     gene            complement(1985..2581)
                     /locus_tag="PKV56_16095"
     CDS             complement(1985..2581)
                     /locus_tag="PKV56_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOX69380.1"
                     /translation="MTSIDKFDPRPLVPVLSFPPELLLSAQGMRAAVFDVDGVLTDGG
                     LYIGEQGEQFKAFNSLDGHGLKLLAQGGITPVVITGRDSPAVRRRIADLGIAHAVYGA
                     RDKLAVAAQVMDELKIGWDTLAVMGDDWPDLPLMTRAAFACAPANAHAEVRAVAHHVT
                     RAAGGSGAARECCDLLLMAAGRYAELLRGHLTTLDRVP"
     gene            complement(2581..3585)
                     /locus_tag="PKV56_16100"
     CDS             complement(2581..3585)
                     /locus_tag="PKV56_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOX69381.1"
                     /translation="MPVHRSVDTAAFDAARALRLAHETFDIEAKALIGLKGRQGANFA
                     RVAEAMLRCTGRVVVMGMGKSGHVGRKIAATLASTGTPAMFVHPAEASHGDLGMVTAE
                     DVVLAISNSGESEEIGALLPALKRLGVTLVSMTGRADSTLASHADIALDCGVDQEACP
                     LNLAPTASTTAQMALGDALAVALLDARGFREADFARSHPGGSLGRKLLIHVRDLMVSG
                     DDVPRVAPEASFGELLREMTGKGLGFTAVVDAAQEVLGIFTDGDLRRLIERGQDLRSL
                     TAQEVMHASPKVIRDEALAVDAAGLMEQHRVTGVLVISAEGRLVGALNLNQLMRAKVL
                     "
     gene            3708..5708
                     /locus_tag="PKV56_16105"
     CDS             3708..5708
                     /locus_tag="PKV56_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOX69382.1"
                     /translation="MASTLELTLLYLVAAVAGVVLCRLAKLPPMLGYLAVGVLIGPNA
                     MALAKDSAGVRYLAEFGVVFLMFVIGLEFNLPKLKSMRKLVFGLGLAQVLLTIIGTLV
                     GHFILAWAFSFTTRSWQLSWQGAVVLASAMAMSSTAIVVKMMADRLEMESEHGRRVIG
                     VLLFQDLAVVPLLVLIPALDDSGRDMMVAFGTAALKATVLLGLLLVGGQKVMRWWLTL
                     VARRKSEELFILNLLLVTLGLAWLTEHFGLSLALGAFIAGMLIAETEYKHQVETDIRP
                     FHDVLLGLFFITIGMKLDWRPVLDQWQLVLVLSLLPVAAKFVLVTVLARLFGTPSGIA
                     LRTGLYLAQAGEFGFVLLTLGAQQRLIEPQWISPVLASMVLSMLASPFLINYSDRIVM
                     RLSASDWLMQSVAMTTIAKRAINTESHVIIAGYGRSGQNLARILEAEHIPYMALDLDP
                     DRVRQAAAAGQSVVFGDAARLQSLMAAGLARASAVVVSYHDTPSALKILHLVQEHAPK
                     VPVIVRTIDDSDLERLQAAGATEVVPEAIEGSLMLASHALALVGVPLRRVIRVVQDQR
                     DARYGLLRGYFHGADDDTFDEIGNMRLHSVTLPVASGCLGQTLGDQALHAVGVSVASV
                     RRASGAVTRPDDNLVLTAGDTLVLSGLPEPLARAEGKLLSRA"
     gene            5772..>6924
                     /locus_tag="PKV56_16110"
     CDS             5772..>6924
                     /locus_tag="PKV56_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX69383.1"
                     /translation="MLRINELRLPLDHPAEALRAAVLGRLGVTDKALKQITVFKRGVD
                     ARKKTAVILTYTIDCEVDAEAEVLARLAGDPQVRPAPDTRYRFIGHAPADFHARGRLR
                     PLVIGFGPCGIFAALVLAQMGLQPIVLERGRQVRERTQDTWGLWRKGILNPESNVQFG
                     EGGAGTFSDGKLWSQISDPRFLTRKVLTEFVRAGAPEEILWVGKPHIGTFRLVGMVEK
                     MRAEIEALGGEVRFGARVTDVRIEGSGESRSIRGVTLSGGQQIDADQVVLALGHSARD
                     TFTMLHERGVCMEAKPFSVGFRIEHPQSLIDRARYGPNAGNPILGAADYRLVHHASNG
                     RSVYSFCMCPGGTVVAATSEAERVVTNGMSQYSRNERNANAGIVVGINPQ"
BASE COUNT         1182 a         2311 c         2261 g         1170 t
ORIGIN      
        1 acatcgagaa gctgatcaag cgccagatcc ccagcgaagt ggtgcccggc ttcgagccgc
       61 cgtccggcga gaaggccgag cccatcgtgc tgggccgcat gaccatcggc gtgggcggca
      121 cccgccgcaa tgccggcggc ggcttcggcg gtggccgccc aggtggcggc ggcggtggtg
      181 gtggtggtgg cggcggtggc cgttccggcg gtggcggcta cggcggcggc ggcggtggaa
      241 accgcggcgg ttactgacct gacccgatca gacccgcttg tcgcgaaaac actggccctg
      301 gggcagtgat cgcgcaaccg agacgctgaa taccgaacgg ctgcacttca aagtgcagcc
      361 gttttgcttt cagccgatgc tcttgggttt tcgatgcttt cggggctgcc ccgccagtac
      421 ccggtcgagc caggcgttcg gcaagacgcg caaaaacccg gccacgatgc ccatttgcca
      481 cgggatcacc cggtagctgg caccctgctc tatcgcatcg acggcccgct cggcaaaagc
      541 tgcggggctc atcagaaagg gcatcggata ccggttgtcg cgcgtcaacg gggtgtcgat
      601 gtatcccgga ctgagcgtga cgaccttgac cccgaacggc cggcattcac cgcgcaggct
      661 ctcgcaatag ctgatcacgg ctgccttgct ggaacagtag gccccatgcc ccggcaagcc
      721 gcgaatcccg gcgacgctcg cgataccaac cagcgttcca ctcctgcgtt cgcagaaagc
      781 acgcacgaag ggctgaaatg tcgcggcgag gcccaggttg ttggtctcga aggtggctcg
      841 catcacctcc atgtcgtcga actccgccgt gtccatgccg atgctgatgc ctgcattggc
      901 gatgacgaca tccgggagcc cctgcccggc aatgcacgcg cgaccggcac cggtgatgct
      961 gtcgatgtcg cgcacatcgg ctgcatacac aacgcaggca gaaggtccga ggcctcttga
     1021 ccgagaccag gccgcgatct ccggggcgcg tcttgccacc aaggcaagtc ggtaacccgc
     1081 ttgcgcgaaa cgctctgcaa gtgccagacc gattccgctg gaggcgccgg taatgtagac
     1141 aagcggcgcg ttcatcaaga ccgtccccgg ggaaagcccg attcgaccaa ctcagcgctg
     1201 ggctgcgctc gctggtctcg agttggaaac tccaggcgat cggtcgactg acctgtcggt
     1261 cgctggaagc ggcgcgccgg gtgtctgcga ggaagggagg aagctggccc tcacacgccc
     1321 attgaactga gccacccgcg acaggttgtc gtagtgcatc gaggcgccct ggatttgagt
     1381 gctcccacgc gtgatcgtca ctgggagatg cgatcgaacc tgctcggtac gcagaaacgc
     1441 atgcaggaac tcgctcctga agtcgactgc ctcctcgacg ccactggccg aggcaaaggc
     1501 ctcacgcacg acatgggcct ggccggtcag ttgcagttcc gtgccgtctc cattcgacaa
     1561 cgcgctcttg gccgtggcca gagtgacatg gccatcgata ccgatcgatc ggacgcgagc
     1621 gttatccatt tccagcgtgt cggtatcggg atagtggcgc aactcatcgc cctcgatctg
     1681 cgccctcaga cggccatcgg cggagaagcg ctggactgtg aactggcgca tcgtgtaatc
     1741 gggaatgtgc cgcggagcga ccgccggctt ttccccctga tacaggggcg tgttcttgac
     1801 cagccaccag gtggccaggg ccagcagcgc catcagcaca agcggcaggt acgccgtcag
     1861 cccgctcagc acgcgctggt accagggcag cacgaagcgc cgctctgccg gctcgtcgac
     1921 cggcaacagg gtcgaatcgg cgaaagtcaa cgagaccacc ttggcaagct gtgcgacatc
     1981 agcttcatgg cacccgatcg agcgtggtca ggtgacctcg caacagctct gcatagcggc
     2041 cggcggccat cagcagcagg tcgcaacatt cgcgcgccgc gccgcttccg ccggcggctc
     2101 gcgtcacgtg gtgtgccaca gcacggactt cggcgtgcgc atttgcggga gcacacgcaa
     2161 atgcggcacg cgtcatgagc ggcagatcgg gccagtcatc gcccatcacc gcgagcgtgt
     2221 cccagccaat cttgagctcg tccatgactt gagccgcgac cgcgagcttg tctcgcgcgc
     2281 catagaccgc atgcgcaatg ccgaggtcgg cgatgcggcg acgcaccgca ggcgagtcgc
     2341 gcccggtgat gaccaccggc gtgataccgc cttgcgcgag cagcttgagt ccatgcccgt
     2401 cgagactgtt gaacgccttg aactgctctc cttgctcgcc gatgtaaagg ccgccatcgg
     2461 tgagcacgcc atccacgtcg aagacggcgg cacgcattcc ctgcgcgctc agcagcagct
     2521 ccggcgggaa ggacagcacc ggaaccagag gacggggatc gaatttgtcg atggaggtca
     2581 tcagagcacc ttggcacgca tcagctgatt caggttcagc gcaccgacca ggcgcccttc
     2641 ggcactgatc acaagcactc ccgtcacgcg atgctgctcc atcaggccgg cagcatcgac
     2701 cgcaagcgct tcgtcgcgaa tcaccttcgg gctggcatgc atgacctctt gtgcagtcag
     2761 cgaacgcagg tcctgaccac gctcgatcag gcgacgcagg tcaccatcgg tgaatatgcc
     2821 cagcacctcc tgcgctgcgt cgacgactgc agtgaacccg aggcccttgc cggtcatctc
     2881 gcgcagcagt tcaccgaagc tggcctcggg cgccacccgc ggaacatcgt cgcccgacac
     2941 catcaggtcg cgcacgtgga tcagcagctt gcggcccagg ctgccgccgg ggtgcgagcg
     3001 tgcgaagtcg gcctcgcgga acccgcgcgc atcgagcagc gcgaccgcca gcgcatcccc
     3061 cagcgccatc tgagcggtgg tgcttgccgt cggtgccaga ttgaggggac aggcctcctg
     3121 atcgacaccg caatccagcg cgatgtcggc gtggctcgcc agcgtcgaat cggctcgccc
     3181 ggtcatgctg accaaagtca ctcccagtcg cttcagcgct ggcaggagcg cgccgatttc
     3241 ctcgctttca cccgaattgg agatcgccag gaccacgtct tccgccgtca ccatgcccag
     3301 gtcgccgtgg ctggcttcgg cagggtgcac gaacatcgcc ggtgtgcccg tcgaggcaag
     3361 cgtcgccgcg atcttgcgtc ccacgtgccc actcttgccc atacccatga caaccacgcg
     3421 cccggtgcag cgcagcatgg cctctgcaac gcgcgcgaag ttcgcgccct ggcgcccctt
     3481 caaaccgatc agcgcctttg cctcgatatc aaaggtctcg tgcgcaagtc gcaaggcgcg
     3541 cgctgcatcg aaggcggcgg tgtcgacgga tcggtggact ggcatgttgc gtgctggggc
     3601 gtccattagg atcgaaccac ataaggtgcc gcattctagg agcctgctac cgccggcggc
     3661 ccgaacagca ccctcgtgcg tcacgtctgc gccggttaca ggaatcgatg gcctccaccc
     3721 ttgaattgac tctcctttac ctcgtcgctg cggtagcagg cgtggtgttg tgccgactgg
     3781 ccaagctgcc gccgatgctc ggctacctgg cggttggcgt gctcatcggg ccgaatgcaa
     3841 tggcgctggc caaggactcg gccggcgtgc gctacctcgc cgaattcggc gtcgtgttcc
     3901 tgatgttcgt gatcgggctt gaattcaacc tgcccaagtt gaaaagcatg cgcaagctgg
     3961 tcttcgggct cgggctggcg caggtgctgc tcaccatcat tggcacgctg gtcggtcact
     4021 tcatcctggc gtgggcgttc tccttcacca cacggtcgtg gcagttgtcg tggcagggcg
     4081 ctgtcgtgct cgcatcggcc atggcgatgt cgagcaccgc gatcgtcgtc aagatgatgg
     4141 ccgaccgact ggagatggaa agcgaacatg gtcgccgcgt gatcggcgtg ctgctcttcc
     4201 aggatctggc cgtggtgcct ttgctggtcc tgatccccgc gctggacgac agcggaaggg
     4261 acatgatggt tgcattcgga acagcagctc tgaaggccac ggtgctgctc ggcttgctgc
     4321 tggtcggcgg gcagaaggtg atgcgctggt ggctcacgct ggtggcgcgc cgcaagagcg
     4381 aggagttgtt catcctgaac ctgctgctgg tcacgctcgg cctcgcctgg ctgaccgagc
     4441 acttcgggct gtcactggca ctgggcgcct tcatcgccgg catgctgatc gccgagaccg
     4501 aatacaagca ccaggtcgaa accgacatcc gccccttcca cgacgtgctg ctcgggctgt
     4561 tcttcatcac catcggcatg aagctcgact ggcgacccgt gctcgaccag tggcagctgg
     4621 ttctggtgct gtcgctgctg ccggtggcgg ccaagttcgt gcttgtgacc gtgctggcgc
     4681 gcctgttcgg cacgccctcc ggcatcgcgc tgcgcaccgg tctgtacctg gcacaggccg
     4741 gtgagttcgg tttcgtgctg ctgacactcg gggcgcagca acggctgata gagccgcagt
     4801 ggatcagccc ggtactggcc agcatggtgc tcagcatgct ggcctcccca ttcctgatca
     4861 actacagcga tcgcatcgtg atgcgcctga gcgcgagcga ctggctgatg caatcggtcg
     4921 cgatgaccac gatcgccaag cgcgcgatca ataccgaatc ccacgtgatc atcgcgggct
     4981 acggtcgcag cgggcagaac ctggcccgca tcctcgaggc ggagcacatc ccgtacatgg
     5041 cgctcgacct cgatcccgac cgggtgcggc aagccgccgc tgccggacaa agcgtggtgt
     5101 tcggcgacgc agcgcgcctg caaagcctga tggctgccgg cctggcgcgg gccagcgccg
     5161 tggttgtcag ctaccacgac acgccctcgg cgctgaagat cctgcacctg gtgcaggagc
     5221 acgcgccgaa ggtgccggtg atcgtgcgca ccatcgatga cagcgacctc gaacggctcc
     5281 aggctgccgg ggcgaccgag gtcgtgcccg aggccatcga gggctcgctg atgctggcca
     5341 gccacgcgct ggcgctggtg ggcgtgccgc tgcggcgcgt gatccgcgtc gtgcaggatc
     5401 agcgcgacgc acgctatggc ctgctgcgcg gctacttcca cggcgccgac gacgacacct
     5461 tcgacgaaat cggcaacatg cgcctgcaca gcgtcaccct gccggtcgcc agcggttgcc
     5521 tgggccagac gctgggcgac caggcactgc acgccgtggg cgtgtcggtg gcgtcggtgc
     5581 gacgcgcgtc gggcgcggtg acccgcccgg acgacaacct ggtgctgacc gccggcgaca
     5641 cgctggtgct ctccggcctg cccgagccgc tggcacgcgc ggagggaaag ctgctgtcgc
     5701 gggcttgaac gctgacacct cgggcttggt cggtggaacc gacaatgcca ggaaagatcg
     5761 acacccttcc catgctgcga atcaacgaac tgcgcctgcc gctcgaccac ccggccgaag
     5821 cgctgcgtgc tgctgtactc ggccgactcg gcgtcaccga caaggcgctg aaacagatca
     5881 ccgtcttcaa gcgtggcgtc gacgcacgca agaagacggc cgtgatactg acctacacca
     5941 tcgattgcga ggtggacgcc gaagccgagg tgctggcccg cttggccggc gacccccagg
     6001 tccgcccggc tcccgacact cgctaccgat tcatcggtca cgcgcccgcc gatttccatg
     6061 ctcgcgggcg actgcggccg ctggtcatcg ggttcggtcc gtgcggcatc ttcgctgctc
     6121 tggttctggc gcaaatgggg ctgcagccca tcgtgctcga gcgcggacgg caggtgcgcg
     6181 agcgcacgca ggacacctgg ggcctttggc gcaagggaat cctgaacccc gaatcgaatg
     6241 tgcagttcgg cgaagggggt gccggcacct tcagtgacgg caagctctgg agccagatca
     6301 gcgacccgcg ctttctgacc cggaaggtgc tgaccgaatt cgtccgtgcg ggcgcccccg
     6361 aggaaatcct ctgggtcggc aagccgcaca tcggcacgtt ccggttggtc ggcatggtcg
     6421 agaagatgcg cgccgagatc gaggcgctgg gcggcgaggt gcgcttcggc gcgcgcgtga
     6481 ccgacgtgcg gatcgaagga agcggcgagt cgcgctcgat ccgcggcgtg acgctgtccg
     6541 gcggacagca gatcgatgcc gaccaggtgg tgctcgcgct cggccacagt gcgcgcgaca
     6601 cgttcacgat gctgcacgag cgcggggtct gcatggaggc caagccgttc tcggtcggat
     6661 tccgcatcga gcacccgcag agcctgatcg atcgcgcccg ctacgggccg aacgcgggca
     6721 acccgatcct cggagccgcc gactacaggc tggtgcacca cgccagcaac ggccgctccg
     6781 tctacagctt ctgcatgtgc ccgggcggca ccgtggtcgc cgccacttcg gaagccgagc
     6841 gcgtggtcac caacggcatg agccagtatt cgcgcaacga gcgcaacgcc aacgccggca
     6901 tcgtggtcgg catcaacccg cagg
//
LOCUS       DAOORV010000174         6784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_13741_length_6784_cov_7.844108, whole genome shotgun sequence.
ACCESSION   DAOORV010000174 DAOORV010000000
VERSION     DAOORV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13741_length_6784_cov_7.844108"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKV56_16115"
     CDS             complement(<1..253)
                     /locus_tag="PKV56_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69384.1"
                     /translation="MDHPIARPARTTPADPALAAVQSATGASSAASPQHKRKPRPGWK
                     ATIVRSESFAELRRIQKSTRDPVIDLSYLADACVELALEL"
     gene            complement(253..972)
                     /locus_tag="PKV56_16120"
     CDS             complement(253..972)
                     /locus_tag="PKV56_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX69385.1"
                     /translation="MNLRLTRVTVACGLATTLVVATASCSFVPRAHETQDQAAPAAAR
                     PAPLRIVQLNFGQNAYFAACAEPACPPVTPKTLPGPVTQPVAATADDLGNRVTASDAA
                     IEPRSPAPPRAASAPAITSRAESVDTHILLTFPFGSATLTAESRQTLRRSLHLARESD
                     RIVISGRTDNIGPDGINQQLALARALAVRNFIRDVVTDLPNVIAIDAKGRCCFIAPND
                     DESGRSRNRRVEVVFTSSGVM"
     gene            complement(969..1532)
                     /locus_tag="PKV56_16125"
     CDS             complement(969..1532)
                     /locus_tag="PKV56_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOX69386.1"
                     /translation="MLHSRALAIVASLAALHAPGVAHACWNDAGARYGVSPHLLYAVA
                     RAESDLNPVAVNLTHRARTKSYDIGLMQINSSHLPTLARFGISERDLYEPCTNIMVGA
                     WLLADQFARQGVSWDSVGAYNAACTQLKGAACRAARSTYAWRVYRRLPGQGTSHRAPK
                     PSTSQPGSSPAVAAHSAPFILSARVTP"
     gene            complement(1536..1994)
                     /locus_tag="PKV56_16130"
     CDS             complement(1536..1994)
                     /locus_tag="PKV56_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69387.1"
                     /translation="MPHNVVAAQPPAILMTRVWLLEHDWLHSVYTSNSNQSPTFRFPD
                     LLSACIELVLSSTESLARLQLFLSTELGARDPKSPRRTCHVWRRQFDELLREHRASWN
                     SHPNPKFDLDAIATGCVAVVRAEHDPIRRVLTQARLNCAARAAAEADQRA"
     gene            complement(2232..2855)
                     /locus_tag="PKV56_16135"
     CDS             complement(2232..2855)
                     /locus_tag="PKV56_16135"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HOX69388.1"
                     /translation="MIRIVWIAALSCVLALVLYIPSAVPPDRLMQTVRSEHELNTTLW
                     GMATADRILERMLTFQAGGMNVSSPPPETVQVAGPGINTAMAVEFGQMSTRLFSSPYF
                     KSVDALFTLAALRAATLLHVLPLLLVFMFVCAVDGFAVRSVRAREFAAHSAEVYTASA
                     TAGIALLSLVLVALFLPFTVSPLYTIGALLLMLFMLSRTIANYHLIR"
     gene            complement(2852..4762)
                     /gene="traD"
                     /locus_tag="PKV56_16140"
     CDS             complement(2852..4762)
                     /gene="traD"
                     /locus_tag="PKV56_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801266.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HOX69389.1"
                     /translation="MLVRPYEMPWRPAYEAWAAAAWLLGLLYFVYVTGSRALFTPFAL
                     TLSAFAMLMMIVRARQAMRVLTVRASLSGRAMQVITTRRLAQLTPDPSLVFLGFGFEW
                     QPLHSQRLYELAKIDYKEFTLPPSLLSLLGYTVDPQPDAEIGMPFIHGVEPKEQPLYR
                     PLQNFEGGTLLVGTTQAGKGVALATLLTQAIKRGDVVVFIDPKNSRRLKRVVQRACQD
                     YRQPDTFLEFHPAFPEVGVRLDFTFNWQKPTEIASRLQSIMPADKDGTFSAFGWDAVN
                     VVVQGLVSLEDRPNLVKLIKYVAGGVEPVLEASLTHFFDRILPRGWRDSVEMRKLLQE
                     AARGQIRRPSEVTSPQLIAYVTYYEQQVPQNQHERVIDDQIRVFRHNREHYQKITASL
                     LPILSMLTSGDLGKSLSPDPFDLDDTRPIMNFEKIERGRHVLYMCLDSLPDPSVASAI
                     GALALADMAARAGMRYNLDIKGRITLVVDEVSNVINKPLIEILNKGAEGGIHSICAMQ
                     TLADLANRLGSEDAARMALGNLNNLFALRTKDRPTQDFVVETFGKTGIHTMRVGRNLG
                     ADTHLGDWSSGRSVQITDAMEERIPADMLGKLPNLQYFAQVSGGRLIKGRFPILNPDF
                     DRSAARSEGAPS"
     gene            complement(4776..6638)
                     /gene="mobH"
                     /locus_tag="PKV56_16145"
     CDS             complement(4776..6638)
                     /gene="mobH"
                     /locus_tag="PKV56_16145"
                     /inference="COORDINATES: protein motif:HMM:NF041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HOX69390.1"
                     /translation="MIVATHDVVWTMGLLDLFQIRQRHRGGPLPRTEPAGLTGLPSSG
                     GHPSADPGICASTVDDLIASESEFLQRFKYCYGCDGETFSRDIVEPIRRYASYVNLLP
                     ATADNFFCTAGGLFRLGLEVAFFALQGTDAHIVSGRATITVRKELEPRWRQATFLAGL
                     CSELHRTLSHATVTDEQGNEWPAYLVPLTVWLERRKARRFFVRWIANAPESRSLSLFA
                     LPHIVSPDSMQHLAAANNVVVPQMLASITGIPLLHEQSILIELVKRSAALVIDRDLAA
                     SAHRYGRPILGAHIERYLLDAMRQLVAAHPAWSPNTERSRVWLGTEGLFIVWPNAAAE
                     IRKVLEDDELRGIPKAPETIVEILTAAGALVAPPEGQACWNILPPGSSSPHEAIRLAS
                     PDILLAGLARSVEVLAQPLVARTSTAHARTAGSAPSIANHPTSVAGSPAAVPAPAANG
                     ACPASPISRPESPMKAEAEQQRPPEDVQATLDFEPPTELAAPSGTATEPNATAAPAAL
                     PTRQFALKAPLRLVPQVRQALQAAVATMNGDPRSAAAITVASGVFVPLSHFKANSIDI
                     SVALRALADVDMLVGTKDRRPRTYQHDIAGQEQAGVIVRPAHIQGLDPADFQAT"
BASE COUNT         1187 a         1975 c         2457 g         1165 t
ORIGIN      
        1 cgagctcgag tgccagctcg acgcacgcgt cggcgagata gctgaggtcg atgactggat
       61 ccctcgtgga tttctggatg cgccgcagct cggcgaagct ttcgctccgg acgatcgttg
      121 ccttccaacc ggggcgaggc ttgcgcttgt gctgcgggga tgcagccgac gaagcgcccg
      181 tggccgattg aacggctgcg agtgcagggt cggcgggagt ggtgcgcgcg gggcgagcga
      241 tggggtgatc catcacatga ctcccgagct ggtgaagacg acttccacgc gacggtttcg
      301 gctgcggccg gattcgtcgt cgttgggcgc gatgaagcag caccttccct tcgcgtcgat
      361 cgcgatcacg ttgggtagat cggtcacgac gtcgcggatg aagttccgga cggccagggc
      421 gcgagccagc gccagctgct ggttgatgcc atcggggccg atgttgtcgg tgcgtcctga
      481 gatgacgatc cgatcggact cgcgcgccag gtgcagagag cggcgaaggg tttggcggga
      541 ctccgccgtg agggtggccg acccgaacgg aaaggtcagg aggatgtgcg tgtcgacgga
      601 ttcggcacgc gatgtgatgg cgggcgccga tgcagcacga ggtggagcag gggatcgcgg
      661 ctcgatggca gcatcagagg cggtcacccg gttccctagg tcatcggcag tggctgcaac
      721 gggctgagtg acggggccgg gaagcgtctt gggtgtgacc ggaggacagg cgggctccgc
      781 acaggcggcg aagtaggcgt tctggccgaa gttcagctgg acgatgcgaa gcggagcagg
      841 gcgcgccgct gccggggccg cctggtcttg tgtctcgtgc gcacgaggca cgaacgagca
      901 cgaggccgtg gcgaccacaa gcgtggtggc caagccgcac gccactgtca ccctggtgag
      961 gcgcaggttc atggtgtgac ccttgcagac agaatgaacg gtgctgaatg cgcggccacg
     1021 gctggcgagc tacccggctg cgatgtggac ggtttcggcg cgcgatggct ggtgccttga
     1081 cccggaaggc gtctgtacac gcgccaggca tacgtggacc gcgcggcgcg gcacgcggcg
     1141 cccttgagct gggtgcatgc ggcgttgtag gcgccgacgg agtcccagct cacgccttgg
     1201 cgtgcgaact ggtcggccag cagccaagcg ccaaccatga tgttcgtgca gggctcgtag
     1261 aggtcgcgct cgctgatccc gaagcgcgcc agcgttggca gatgcgagct gttgatctgc
     1321 atgagcccga tgtcgtagct cttcgtgcgg gcacgatggg tcaggttcac tgccaccggg
     1381 ttgagatcgg actcggctct ggctactgcg tagagcaagt gcggcgacac accgtaacgg
     1441 gcaccggcgt cgttccagca agcgtgagcg acgccggggg cgtgcagggc agcgagggaa
     1501 gccacgattg caagtgcgcg tgaatgcagc atggttcagg ctcgctggtc agcctctgcc
     1561 gcggcacgag cggcgcaatt gaggcgcgcc tgggtaagca ccctgcgaat cgggtcgtgc
     1621 tctgcgcgaa ccaccgcaac gcaccctgtc gcgatggcat cgaggtcgaa cttcggattc
     1681 gggtgggagt tccacgaggc ccggtgttcg cggagcaact cgtcgaactg gcggcgccac
     1741 acgtggcaag tgcgccgtgg cgacttcgga tcgcgcgcac cgagctcggt gctcagaaag
     1801 agctgcagtc gggccagact ttcggtgctg gaaaggacca gctcgatgca ggccgagagc
     1861 agatcgggaa aacgaaaggt cggcgactgg ttggagttgc tggtgtagac gctgtgcagc
     1921 cagtcgtgct cgagcaacca aacgcgtgtc atcaagatgg ccggaggctg tgcggcgacg
     1981 acgttgtgcg gcatcgatga acctcgtgag tgagcgactt cggtgtgcat gtgctgtagc
     2041 cccgatgagg cccgcgacat gcgacacctc aggtcgagag cgtagaaagc aattcgatgt
     2101 cgctcaactc gaaacgcggc accaagtccc ccgaaatttc gaccgtcgat ttcgcctgtt
     2161 gccagtcagc gcccgcaaac gaagcggccc gcgtgagggc gggccgctgg actcaagtcg
     2221 ctgggtgcag gtcaacgaat caggtggtag ttcgcgatcg tgcggctcag catgaagagc
     2281 atcagcagca gggccccgat cgtgtacagg gggctcacag tgaagggcag gaacaaggcg
     2341 accagcacga gtgacagcag cgcgatgccg gccgtggcac ttgcggtgta gacctcggcg
     2401 ctgtgagctg cgaactcgcg ggcccgcacg ctgcgtaccg cgaacccgtc gacggcgcac
     2461 acgaacatga aaaccagaag cagtggaaga acgtgcagaa gcgtcgcagc acgcagggcg
     2521 gccagggtga acaacgcgtc gacggacttg aagtagggcg aggagaacag gcgcgtgctc
     2581 atctgtccga actcgaccgc catggcggta ttgattccgg ggcctgccac ctggaccgtt
     2641 tcgggtggcg gagaagagac gttcatgcca ccggcctgga aagtcagcat ccgctcaaga
     2701 atccggtcgg cggtggccat gccccaaagc gtcgtgttga gttcgtgctc ggacctcacg
     2761 gtctgcatca gccgatcggg cggaacggcc gaggggatat agagcacaag ggcgagcacg
     2821 cagctcaggg cggcgatcca gacgatgcgg atcatgacgg cgccccttcg ctgcgcgcgg
     2881 ccgagcggtc gaagtcggga ttcagaatcg ggaagcggcc tttgatgagc cggccgcccg
     2941 atacctgtgc gaagtactgc aggttgggga gcttcccaag catgtcggcc ggaatgcgct
     3001 cttccatcgc gtcggtgatc tggaccgacc ggccgctgct ccagtcaccc aggtgggtgt
     3061 cggcgccgag gttgcggccg acccgcatgg tgtggatgcc ggtcttgccg aaggtctcga
     3121 cgacgaagtc ctgtgtgggc cgatcctttg tgcgcagggc gaagaggttg ttcaagttgc
     3181 ccagggccat ccgggccgca tcctcagagc ccagccggtt ggccagatcc gccagggtct
     3241 gcatcgcgca gattgagtgg atgcctcctt cagcaccctt gttcaggatc tcgatcaagg
     3301 gcttgttgat gacgttcgaa acctcatcga cgaccaacgt gatgcggcct ttgatgtcca
     3361 ggttgtagcg catgccagca cgtgcggcca tgtcggccag ggccagggcg ccgatggccg
     3421 atgccacgga cgggtcgggc agcgagtcca gacacatgta cagaacatgg cggccgcgct
     3481 cgatcttctc gaagttcatg atcggccggg tgtcatcgag gtcgaatggg tccggcgaga
     3541 ggctctttcc gaggtcgccg gaagtgagca tggacaggat cggcagcagg ctcgcggtga
     3601 tcttctggta gtgctcgcgg ttgtggcgaa agacgcggat ctgatcgtcg atcacacgct
     3661 catgctggtt ttgcgggacc tgctgctcgt agtaggtgac gtaggcgatc agttgggggc
     3721 tggtgacttc ggagggcctt ctgatctggc ctcgggcggc ctcctgcagg agtttcctca
     3781 tctcgaccga gtctcgccat ccacgcggca ggatgcggtc gaagaagtgc gtgagggagg
     3841 cctccagcac cggctccacg ccgcccgcaa cgtatttgat gagcttgacc aggttgggtc
     3901 gatcctccag gctgaccagc ccctggacca caacgtttac ggcgtcccag ccgaatgcgc
     3961 tgaaagtgcc gtccttgtcg gcgggcatga tcgactggag gcggcttgcg atttcggtgg
     4021 gtttctgcca gttgaaggtg aagtcgagcc ggacgccgac ttcggggaac gcggggtgga
     4081 actcgaggaa ggtgtcgggc tggcggtagt cctggcaggc tcgctggacg acccgcttga
     4141 gccggcggct gttcttgggg tcgatgaaca ccacgacgtc gccgcgcttg atggcctggg
     4201 tcagcagagt ggccagtgcg acgcctttgc cggcctgcgt ggtgccgacc aacagggtgc
     4261 cgccctcgaa gttctgcagc ggccggtaca gcggctgctc cttgggttcc acgccgtgga
     4321 tgaagggcat gccaatttcc gcatcgggct gcggatcgac ggtgtagccc agcagggata
     4381 gcagcgacgg cgggagtgtg aactccttgt agtcgatctt ggccagctcg tacaggcgct
     4441 gggagtgcag cggctgccat tcgaatccga accccagaaa gaccaggctg gggtcgggtg
     4501 tcagctgagc gaggcgtcgc gtggtgatga cctgcatcgc tcgtccggat agcgaagcgc
     4561 gcaccgtcag cacccgcatc gcctggcggg cgcgaacgat catcatcagc atcgcaaacg
     4621 cggagagtgt cagcgcgaac ggcgtgaaca gcgcgcgtga tcccgtgacg tacacgaagt
     4681 acagaaggcc gagtagccag gcggccgcag cccaggcctc gtacgcgggc cgccacggca
     4741 tttcataggg tcggacaagc atggcgttgc cttactcagg ttgcttggaa gtcggccgga
     4801 tcgagcccct ggatgtgggc tggcctcacg atgacgccgg cctgctcctg gcccgcgatg
     4861 tcgtgctggt aggttcttgg ccgacggtcc ttggtgccga ccagcatgtc cacgtcggcg
     4921 agggcacgca gcgcgacgct gatgtcgatg ctgttggcct tgaagtggga cagcggcacg
     4981 aagacgccgg acgcgacggt gatggccgcg gcggaccttg ggtcgccatt catcgttgcg
     5041 acggcggctt gcagggcctg gcggacctgt ggcaccaggc gcaatggagc cttcagcgca
     5101 aactggcgcg ttggtagggc tgccggcgcc gccgtggcgt tgggctctgt cgcggtgccc
     5161 gatggggccg ccagctctgt tggtggttcg aagtcgagcg tggcctgcac gtcctcaggc
     5221 ggccgctgtt gctccgcctc cgctttcatg gggctttcgg gccgggagat tggggaggcg
     5281 gggcatgctc cattggccgc aggcgccggc accgccgccg gtgagcctgc aaccgatgtc
     5341 gggtgattcg cgatggaggg cgcagagccg gcagttcgcg cgtgtgccgt ggacgttcgc
     5401 gcaacaagtg gctgtgccag aacctcgacc gaccgagcca gaccagccag caggatgtcg
     5461 ggcgacgcca ggcgaatggc ctcgtgcggc gacgagctgc ccgggggcaa gatgttccag
     5521 catgcctgcc cttcgggcgg cgcgacaaga gccccggcag cggtgaggat ctcgacgatc
     5581 gtctccgggg ccttgggaat gccgcgaagc tcgtcgtcct cgagcacctt gcggatctcc
     5641 gcggcagcgt tgggccagac gatgaacagc ccctcggtgc cgagccacac ccgcgatcgc
     5701 tcggtgttgg gtgaccatgc tgggtgggcc gcgaccagtt ggcgcatcgc atcgagcagg
     5761 tagcgttcga tgtgcgcacc gaggatcggc cggccgtagc gatgggcgct ggccgccagg
     5821 tcacgatcga tgaccagcgc cgcggaccgt ttcacaagct cgatcaggat gctctgctcg
     5881 tgaagcagtg ggatgcctgt gatggaggcc agcatctgcg gcacgacgac gttgtttgcc
     5941 gcggcgaggt gctgcatcga gtcgggcgac acgatatgcg ggagggcaaa caggctcagg
     6001 ctgcggctct ccggcgcatt ggcaatccag cgaacgaaga agcgacgggc cttgcggcgc
     6061 tccagccaga ctgtcaatgg aaccaggtag gcgggccact cgttgccctg ttcatcggta
     6121 accgtggcgt ggctgagcgt tctatgcagt tcgctgcaca ggcctgcgag aaatgtcgcc
     6181 tgtcgccagc gcggttcgag ttccttgcgc acggtgatgg tggcccgacc ggacacgatg
     6241 tgtgcgtcgg tgccttgtag ggcgaagaag gccacctcca ggcccaggcg aaagagtccg
     6301 ccggccgtgc agaagaagtt gtcggctgtc gcaggaagca ggttgacgta cgaggcgtag
     6361 cgacggatag gctcaacaat gtcgcggctg aaggtttcgc cgtcgcagcc gtagcagtac
     6421 ttgaagcgct ggaggaactc gctctccgaa gcgatcagat catcgaccgt cgacgcgcag
     6481 atgccggggt ccgcggaggg atgtccaccg ctcgaaggca ggccggtgag accagcgggt
     6541 tcggtccgcg gcagtggacc gccgcgatgc cgctggcgaa tttgaaacag atcgagcagg
     6601 cccatggtcc acaccacgtc atgagtggcg acgatcatcc ggcgagaggg gcaggcgctt
     6661 caactcgaaa ggccggtcgt gctccccccg gaaatgcgcc gtcggtggac gcgtcgcgcg
     6721 gatcggtagc tcaggtcacg cggcgagcgc aggcagcagg atccgatggc gccggatgag
     6781 catg
//
LOCUS       DAOORV010000175         6729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_13876_length_6729_cov_12.074019, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000175 DAOORV010000000
VERSION     DAOORV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13876_length_6729_cov_12.074019"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PKV56_16150"
     CDS             <1..1175
                     /locus_tag="PKV56_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOX69391.1"
                     /translation="LSQAVLLDTYPRERHGSAMAMWGVGVMVGPILGPSLGGWLTEYY
                     NWRWVFYINLPFGLLAWFGLAAFVHETPIDRARRFDLFGFALLSLAIGALQMMLDRGE
                     SLDWFSNTEIMIEALLAGVALYMFVAHIFTHEHPFIEPALFRDRNFSVGLVCIFIVGV
                     ILLATMALLPPFMQNLMGYPVIDVGVLLAPRGVGTMLAMITVGRLSGKVDARYQVALG
                     LLLTSLSMWEMTQFTTDTTGPTFIRTGIVQGLGLGFMFVPLSTITFSTLAPRFRNEGT
                     ALFSLMRNIGSSIGISVVVTQLARNTQANHAAFSEFIQPFSMALKQAIEAGVYNLSTP
                     QGLVALNAEVTRQAATLAYLQDFRLMMWVALAALPLILLLRPAAPAGMQQTPAAVE"
     gene            1296..1769
                     /locus_tag="PKV56_16155"
     CDS             1296..1769
                     /locus_tag="PKV56_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX69392.1"
                     /translation="MFQTIVLAYDGSAECRDALADGIALATRFNACCKLLAVVPPMNS
                     MAIASGYMPEGLLDSDQAHYNGILEEGVSRLRQAGVQATGTLKTWEDPSQAIGAFAQE
                     TGADLVVIGHHRRSTLERWWRGSVGHHLLDHLPCSLLVSMHDDAAKRALAGSGDT"
     gene            complement(2008..2679)
                     /locus_tag="PKV56_16160"
     CDS             complement(2008..2679)
                     /locus_tag="PKV56_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX69393.1"
                     /translation="MSTTLRRRLKSQRPRQLLEAALEVFVDKGFAAARVDDVAAHAGV
                     SKGTLYLYYPGKDALLKAVIAHYLGNAIAVGAEELGRHDGPAAEVLCNSMARWWRTIF
                     DSPASAVFMLVVSEVRHFPEIAEFYVHEVVEPGQRLIGDLLRRGIERGEFRDVDVNET
                     VYSLVLPMVMLCLHKHSLGACATGLPAVEPHAFIRHHFEFAVRALMPVPAGSSAAQVP
                     PVQVH"
     gene            3027..3359
                     /locus_tag="PKV56_16165"
     CDS             3027..3359
                     /locus_tag="PKV56_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69394.1"
                     /translation="MSILTIAMRSASLSITSLAALAASAACCIDFSRGSDDTAGLHRI
                     EGVTVFLDHPELTGRDLLQAVRGYEKRGAIVYERVASSIYSRRMLESFGMSGAPRSTL
                     LTPILCDL"
     gene            complement(3412..4986)
                     /locus_tag="PKV56_16170"
     CDS             complement(3412..4986)
                     /locus_tag="PKV56_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX69395.1"
                     /translation="MSYDLPEASPPPFTIGAWTVHAMHDRVERDGQSHKLEPRLMRLL
                     LTLARAGGAVVPAASLLATVWAGVVVTPSSLYESISRLRKLLGHDEHGHEALESVPRK
                     GYRLRWPVSMGSSPGLGERSIAVLPFEAVDLDDALQFIPQSLVDGLIGELSRQPELAV
                     VARGTMIEYARRRESPVSLGRHLGVRSVIDGRIRRCSPGLLSIEAELIDVPSGRQTWA
                     ETIELPDSHWSQSAAWVVGRLARALTFEAQAGVLSRGNEVAVEALPARSLATQAWVEL
                     FARPETAETNGRARGWAAEALQRDPQCGLAEICLATCDWRAGQFGWDGLKEEEARGRA
                     HDHAERAVVLAPSEPDAHYVLALIAYGRGETVRAEEALRQCLRLSASFAPAYGLLALV
                     RTRLGHPEEAPALCDRALALSPREPLRAVWYLALAWSALARKDYAAGLDAAQRGMAAN
                     PGLPTNYAAGAAAAQALGEKDLAARWVQRLREHSVFRSVPAFHRRMPVATSPAHRRQM
                     QEAAHLLAQAGLPEVE"
     gene            5079..5456
                     /locus_tag="PKV56_16175"
     CDS             5079..5456
                     /locus_tag="PKV56_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69396.1"
                     /translation="MDTIRRTAGPQACTASTTNGMVSRVPSQPSAIARSRYLAALGAL
                     FTLFSSARVLAYLPTIWAVLASGDSSQHSLLTWFTFFGGNSTMAAWLWEQNGRRCNAA
                     IAASGGNALMCVVIIAVVTWTRL"
     gene            5603..6514
                     /locus_tag="PKV56_16180"
     CDS             5603..6514
                     /locus_tag="PKV56_16180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOX69397.1"
                     /translation="MTALSTLVGAAVAQQGGQAVRIVVPFAAGSAPDIVARTIADPLA
                     GEFGAPVTIENLPGAGGMLGVTHVANAAPDGRTVVMSGDAALVMPDPNRVRRYDPQRD
                     LAPISRLVVTPNVLVVGVDTESRNFSDWLAWLRLQPPGRISYASAGVGTSSHRAGELL
                     AKMTGVEMTHVPSNQSPLPDVVAGRVDLLFANIATAMPLVREGRLRAFAVSSLQRTSI
                     APELPTLAESGLPGFEATAWFAMLAPAATPPEMLARWQRAARTALATPAVRAKLQSLG
                     IEAVGGSPEELRESIERESRKWAGLIR"
BASE COUNT         1142 a         2286 c         2151 g         1150 t
ORIGIN      
        1 cgttgtcgca ggcggtgctc ctggacacct acccgcgcga gcggcacggg tcggccatgg
       61 cgatgtgggg cgtgggcgtg atggtcgggc ccatcctcgg gccgtcgctc ggtggctggc
      121 tgaccgagta ctacaactgg cgctgggtct tctacatcaa cctgcctttc ggcctgctcg
      181 cctggttcgg gctggcggcg tttgtgcatg agacgccgat cgatcgtgcc cggcgcttcg
      241 atctgttcgg cttcgcgctg ctcagcctgg ccatcggggc actgcagatg atgctggacc
      301 ggggtgaatc gctcgactgg ttcagcaaca ccgagatcat gatcgaggcg ctgctcgcag
      361 gcgtggcgct gtacatgttc gtggcgcaca tctttaccca cgagcatccg ttcatcgagc
      421 cagccctgtt cagggaccgc aacttcagcg tcgggctggt gtgcatcttc atcgtcggcg
      481 tgatcctgct ggcgacgatg gcactgctgc cgcctttcat gcagaacctg atgggctacc
      541 cggtcatcga cgttggcgtc ctgctggcgc cgcgtggcgt cggcaccatg ctggccatga
      601 tcacggtggg gcgcctgtcc ggcaaggtcg acgcgcgcta ccaggtcgcg ctcggcctgc
      661 tgctgaccag cctgtcgatg tgggagatga cgcagttcac gaccgacacc acgggcccga
      721 ccttcatccg cacaggcatc gtgcagggcc tgggcctggg tttcatgttc gtgccgctgt
      781 cgaccatcac cttctcgacg ctggcgcccc gcttccgcaa cgaaggcacg gcgctgttca
      841 gcttgatgcg caacattggc agcagcatcg gcatctccgt cgtcgtcacc caactggccc
      901 gcaacacgca agccaaccat gcggcgttct ccgagttcat tcaacccttc agcatggcgc
      961 tgaagcaggc catcgaagcg ggggtctaca acctgtccac accacaaggc ctggtggcgc
     1021 tcaatgccga ggtcacgcgg caggccgcga ccctggccta cctgcaggac ttcaggctga
     1081 tgatgtgggt cgcgctggcc gcgctgccct tgatcctgct gctgcggccg gcggcgcctg
     1141 ccggcatgca gcagacccct gccgctgtcg agtgacatgg cccgcttcca cgcccacagt
     1201 gcccggaatc agaaggtcac acgccgaagg gcgcgaccgc ctgcctcgcg ccaggcaaag
     1261 agtgtcagtg ttcaggcttc ccaaggagaa catccatgtt tcaaaccatc gtcttggcct
     1321 atgacggcag cgccgagtgc cgcgacgcgt tggccgacgg tattgccctg gcgacccgat
     1381 tcaacgcttg ctgcaaactg ctggccgttg ttccaccgat gaactcgatg gccattgcct
     1441 ctggatacat gcccgaaggc ttgctcgaca gtgatcaggc gcactacaac ggcattctcg
     1501 aagaaggcgt ctcgcgattg cgccaggccg gcgtgcaggc aaccggcacg ttgaagacct
     1561 gggaggatcc gagccaggcc atcggcgcct tcgcgcaaga gaccggggcg gacctggtgg
     1621 tcatcggaca tcaccggcga tcgacgttgg agcgctggtg gcgcggatcg gtcgggcacc
     1681 atctgctgga tcatctgccg tgcagcctgt tggtttcgat gcatgacgat gcagcgaagc
     1741 gggcgctggc gggcagtggc gacacctgaa acccgttgaa tccagcgcga cggccgtcgc
     1801 gccttgggcc tctgccagtg tcggccatgg tagagggggg atccgcacgg atatcacgtc
     1861 gtggcccact ggacgcgctc acgcaccacg gttgcccgcc ccggctgacc cgcagcccac
     1921 gcgccgggca agggtcgcgg tggcgcgcct ttaccttgca cattgcgaca ggacggcgcg
     1981 gccatctcca ggaaacagaa gcacccttca gtgcacctga accggcggca cctgcgccgc
     2041 cgacgagccc gccggaaccg gcatcagtgc acgtaccgcg aactcgaaat gatggcggat
     2101 gaacgcatgc ggctccaccg ccggcaggcc cgtcgcacat gcgcccagcg agtgcttgtg
     2161 caggcacagc atcaccatcg gcagcaccag cgaatacacg gtttcgttga cgtccacgtc
     2221 gcggaactcg ccgcgctcga tgccgcgccg aagcaggtcg ccgatcaggc gctggccggg
     2281 ttccaccacc tcgtggacgt agaactcggc gatctccggg aaatggcgca cctcggacac
     2341 gacgagcatg aacaccgccg aggcggggct gtcgaagatc gtccgccacc agcgcgccat
     2401 cgagttgcac agcacctcgg ccgcagggcc gtcatggcgc cccaactctt ccgcgccgac
     2461 ggcgatcgca tttccgaggt agtgcgcgat caccgccttc agcagcgcat ccttgccggg
     2521 gtagtacagg tagagcgtgc ccttggacac gcctgcgtgg gccgccacgt cgtccacgcg
     2581 ggctgcggca aagcctttgt cgacgaaaac ctcgagtgcc gcctcgagca attgacgcgg
     2641 ccgctggctc ttcaggcgcc ggcgcagcgt ggtgctcatc gcgagcgtgc cggcgcgcgg
     2701 gtcgcggccc aggagtgcaa ggctttgaag tgggagtcga tggcacgcga ccaggccgga
     2761 ccaggccatc ctttgccgtc tcgaacgggc tgatcttgtc cacgaattgc ccatcgttcg
     2821 cgccacgtgt aacgcgggca gccagcgcaa cgtcctgcag cggtgtgcgc ctgcggtctg
     2881 ccaagacgaa tttctaacgc gtacgttttc gcgagcatat aacgtcgccg gctcgggttc
     2941 ttcaccgtcg atggctgatc atggcccaaa gttcctgccc acaacacggg ggtcggtgct
     3001 gcaacgccac gatccactac accgccgtgt ccatcctgac cattgccatg cgttccgctt
     3061 cgctttctat cacatcactt gcggcgctcg ccgcgagcgc ggcctgctgc atcgacttct
     3121 cgaggggcag cgacgacacc gcgggcttgc accggatcga aggagtgacg gtgttcctcg
     3181 accacccgga actgaccggg cgcgacctgc tccaggcggt ccgggggtac gagaagcgtg
     3241 gcgccattgt ctacgagcgc gtcgcgtcga gcatctactc acgccgcatg ctggagtcct
     3301 tcggcatgag tggcgccccg cgatctacgt tgctaacgcc aatactgtgc gacctgtgac
     3361 cttggggcga tgcgctttct caacggcata ggctgatacc tgactgccag gtcattcgac
     3421 ctctggcagc ccagcctgcg caagcaaatg ggccgcttcc tgcatctggc ggcgatgagc
     3481 cggtgaggtc gcgaccggca tgcgccgatg aaatgccggc acactgcgaa agacagagtg
     3541 ctctcgaagc cgctgaaccc agcgcgcggc caagtccttc tcgccgaggg cctgcgcagc
     3601 cgcagcgccg gcggcatagt tagtcggcag gcccggattc gccgccatgc cgcgctgtgc
     3661 ggcatccagt cctgcggcgt agtcctttcg cgcgagcgcg ctccacgcga gagccaggta
     3721 ccagacggca cgcagcggct cccgcggact cagcgccagc gcgcggtcgc acagcgccgg
     3781 cgcttcctcc gggtgcccga gccgggtgcg caccagcgcg agcagcccgt aggctggggc
     3841 gaacgatgcc gacaaccgaa ggcattgtcg aagtgcctcc tccgcacgca cggtttcgcc
     3901 gcggccatag gcgatcaatg cgagcacgta gtgagcatcg ggttcgctcg gcgcgagcac
     3961 gacagctcgt tcggcgtggt cgtgggcgcg tccgcgagcc tcctcctctt tcaacccatc
     4021 ccaaccgaac tggccggcgc gccagtcgca ggtggccagg cagatctcgg ccaggccgca
     4081 ctgcggatcg cgctgcaatg cctcggccgc ccatccgcgg gcacgcccat tcgtctcggc
     4141 cgtttcgggc ctcgcgaaca gttccaccca agcctgggtg gccaacgaac gcgccggcag
     4201 cgcctcgacg gcgacctcgt tgccgcgcga cagcaccccg gcctgggcct cgaaggtaag
     4261 cgcgcgtgcc agccggccga ccacccacgc cgcgctttgc gaccagtggc tgtcaggcag
     4321 ttcgatcgtc tcggcccagg tctgccgccc gctgggcacg tcgatcagct cggcctcgat
     4381 cgagagcagc cccgggctgc agcggcggat gcgtccgtcg atcacagaac gtacaccgag
     4441 gtgccgaccc agcgaaacgg gagactcgcg ccgccgtgca tattcgatca tcgtgccgcg
     4501 cgcgaccacg gccaactcgg gctggcgcga caactcgcca atcaggccgt ccaccagact
     4561 ttgcgggatg aactgcaacg catcatccag atcgaccgcc tcgaagggca gcacggcgat
     4621 ggacctttca ccgaggccgg gcgaacttcc catcgatacc ggccagcgca gccggtagcc
     4681 cttgcggggc accgattcga gcgcctcgtg gccgtgttcg tcgtgcccca acagtttgcg
     4741 cagtcgcgaa atgctctcgt acagcgaact gggggtgacg accacaccgg cccagaccgt
     4801 ggccagcagc gaagctgccg gcaccactgc gccgccagcg cgggccagtg tcagcagcag
     4861 gcgcatcaag cgtggctcga gcttgtgcga ttggccatca cgttcaactc ggtcgtgcat
     4921 cgcgtgcacg gtccacgcgc cgatcgtgaa ggggggtggc gaggcttcgg gtaggtcgta
     4981 ggacaaggcg gctcctgctt gcatcttaag aaaaccccaa aactacgcca gtgctctgtc
     5041 tcccacactg gcagcacacc tcgcaaagga gacacgccat ggacacgatt cgacgaactg
     5101 ccggcccgca agcttgcacg gcatcgacaa cgaacggcat ggtctcccgc gtcccctcgc
     5161 agccatcggc gatcgcacgc agcagatacc tggcggcgct gggagccttg ttcacgctgt
     5221 tcagttcagc gcgcgtgctg gcctacctgc cgacgatctg ggcggtcttg gcgagtggag
     5281 actccagcca gcactcgttg ctgacctggt tcaccttctt cggcggcaac tcgaccatgg
     5341 cggcctggct gtgggagcaa aacggccgcc gctgcaacgc ggccatagca gccagcggtg
     5401 gcaatgccct gatgtgtgtg gtcatcatcg cggtggtgac gtggacacgg ctgtgaatgc
     5461 atcctcccat agctcgcaag cagacccgaa cttaagaaaa gcccaagacc acgccgccgc
     5521 tgcaccccgc acactcgtgc cgcaccatgt caacacacgc cggagttgca ccatgcaccg
     5581 catcaatcgt aggagtgtta tcttgacagc gctctccacc ttggtcggcg ccgccgtcgc
     5641 acaacagggg ggccaggcag tgcgcatcgt cgttcccttt gcggcgggct ccgcaccaga
     5701 catcgtcgcc cggacaatcg ccgatccact agccggggaa tttggggcgc cggtgacgat
     5761 agagaacctg cctggtgccg gcggaatgct tggggtaacg catgtcgcga acgctgcccc
     5821 cgatggccgc acggtcgtga tgtccggcga cgcggctctg gtgatgccag acccgaaccg
     5881 tgtgcggcgc tacgaccctc agcgcgactt ggcgccgatc tcgcgcctcg ttgtcacgcc
     5941 caatgtgctg gtggtgggtg tcgacaccga gtctcggaac ttctcagact ggctggcctg
     6001 gctgcgcctg cagccgcctg gccggatcag ctacgcgtcg gccggtgtcg gaacgtcatc
     6061 gcaccgtgcc ggcgaattgc tggcaaagat gacgggcgtc gagatgaccc atgtgccgtc
     6121 gaaccagagt cctttgccgg atgtcgtagc aggacgcgtc gacttgctct ttgccaacat
     6181 cgcaactgcg atgccgctgg ttcgcgaagg tcgtctgcgt gcattcgcag tgtcatcgtt
     6241 gcaacgcacg tccatcgcgc cagaactgcc gacgctagcc gaatccgggc tgccgggctt
     6301 cgaggcgacg gcctggttcg cgatgctcgc gcccgcggcc acgccgccgg agatgctggc
     6361 gcgctggcag cgtgcggccc ggactgcgct cgcaacgcct gcggtcagag ccaagctgca
     6421 gtctctgggc atcgaggcgg tgggcggatc gcccgaagaa ctccgtgaat ctatcgaacg
     6481 cgagtcacgc aagtgggccg gcctgatccg gtgatcgcac tctgctgaag taactccgac
     6541 gaagccccgg ggagcggccg cccgagatca gcgacggtcg ggtaccccgc cgaagtgcgc
     6601 acacatgacc gccgcctctt cgccgcacac cgcatcaatg cttggaccgg ttcggctggc
     6661 cgattttcat ggtgccaaat gacgtcctga taagaacacc tttcgggacg tgtcgttgct
     6721 gggcgcggc
//
LOCUS       DAOORV010000176         6630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14134_length_6630_cov_6.508897, whole genome shotgun sequence.
ACCESSION   DAOORV010000176 DAOORV010000000
VERSION     DAOORV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_6630_cov_6.508897"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKV56_16185"
     CDS             <1..803
                     /locus_tag="PKV56_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HOX69398.1"
                     /translation="ADAVKAVAMMPAPLRRFSTVRQNLPKAGENSLFERLGRWCQGGA
                     LGWVFDQADDRLLDLHAANVIGFDTTEFLDLPEVRTPVMMYLLQVMEELVNGERLIYV
                     ISEFWKALDHEIFSDFAKQKQKTIRKQNGLGIFDTQSPSDVLRHPIGRTMIEQSVTKI
                     FLANPEAVREEYVDGFGLTQAEFDIVRSLGAQGGRRFLVKQGHGSAICELDLAGLEDF
                     ITVLSATTDNVALLDAVRERHGNDPFQWLPVLLREVQDRRFRTARRAA"
     gene            800..1228
                     /locus_tag="PKV56_16190"
     CDS             800..1228
                     /locus_tag="PKV56_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69399.1"
                     /translation="MNAVHHLLALGALALAASTASAQFVKGNEAVKVMPDGTKKVETP
                     PLPTTGPIRSTKPCAADDKCHGGAWLMVETKDGLVECTEAYARPGTCRKSTYGETKLL
                     RLWVVSSGGKWLQCQRPDTSSKCVDMFARPPGNLPFPATQ"
     gene            1241..2326
                     /locus_tag="PKV56_16195"
     CDS             1241..2326
                     /locus_tag="PKV56_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HOX69400.1"
                     /translation="MFNWVGLQLDGILSTYVLNVVSTLMAGLAPIALICMTIWVMLYG
                     WAVLRNEVSETLPVFMWKAFKIGLVLAFALQSGFYISNVADTANGLAMGVASTFLPSG
                     VDPVTVTSPYALLDKFNDDASAQVADIMKEASMFRLDLLLAAAIFSIGSVVFLCIALF
                     VVTLSKLFLTFVIAIGPLFVLCLAWRPTARFFDSWLSMVLNAVVLTWFAFFALGLSAF
                     MGVSMFKAIQDGGGFLGGTFNVLGEATRYCVLMILMAIICFQAPSLASALTGGAAVQQ
                     GVQMIQNTMMVSGLRSGTGRGSSGNPTSGGVVRAGTGPAYAAGAYAAAAGRAARTATS
                     GASGAARQGFAAARTAAYKLAAMRGRT"
     gene            2353..3051
                     /locus_tag="PKV56_16200"
     CDS             2353..3051
                     /locus_tag="PKV56_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HOX69401.1"
                     /translation="MKLRLKAAAAATAIVLGTGNAQAQGIPVIDIANLIQTLLQVLND
                     ITEISNQVQQIYQLENQVKSLNGIRNLGDVFNNPMLRNYIPAEAYTQLNAIQNSGYAA
                     LNGTAKALRDAGMVYNCMDLTGAARTTCQSTLAQPYQHKGLLQDAMKSASGRLSQIQS
                     LMNQINATTDQKAVQEIQARIGAENALLAHEVSQVQMLQGMADSEERIARSRERERQY
                     EMLVRTGKVADYLP"
     gene            3102..3875
                     /locus_tag="PKV56_16205"
     CDS             3102..3875
                     /locus_tag="PKV56_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HOX69402.1"
                     /translation="MSAVLTPGKAAALPDRSRHTGKAAAESTAIDANRAWEVDRALML
                     ERSERRAWWVAIAGLVLGLIGMAGVFVQGPLRRVVEIPIVVDRVTGEATIQQRLAVET
                     IPPMEALDKHNLATFVRAREGYSWMFLQRDFDQVARMAVPAVFADYNRQFEGDGALQK
                     KIGAAEDWRINVIGVRLAASGRAGNKGEATVTYDKVVRLTDRNLPEVTTRHVASVVYQ
                     YQPKVLAKERDRLENPFGFVVTAYRSDPEINTAGPGAKP"
     gene            3872..4792
                     /locus_tag="PKV56_16210"
     CDS             3872..4792
                     /locus_tag="PKV56_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HOX69403.1"
                     /translation="MKPLAWLAAFACAAPCVIATAAPDVADPRLREVVYDPRAVVTVP
                     VKRGVVTLVVLDPDESITEVAAGLGGDCTKADAAWCVAAQPGGRNLFVKAKSSARAAN
                     NLAVVTDRRTHTFRFVVLADGDPKPPVYRLVVKTPPRQAPPAQLALREDVPLVALPAV
                     PPPPPPQQVVAERLQAKPLVTNTQYSIAEGASSQDIVPTLVYDDGRFTYLRFPGNREV
                     PAVFHVLGDGSETLVNARMEDDLLVVDRVSRKLMLRAGQAVVGLWNEAFDLDGRPPGD
                     GTTVPGVQRVLKADAGGNNAPSGARKGVTP"
     gene            4789..6234
                     /gene="virB10"
                     /locus_tag="PKV56_16215"
     CDS             4789..6234
                     /gene="virB10"
                     /locus_tag="PKV56_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HOX69404.1"
                     /translation="MTQDPLDQEPGVDADAAADGNSISPLPGEPGIPNVAARQRVSTS
                     KKGLLAVALLLGSLVAVAAFTVQRVSVSGKQAEEPESNLVKDKPTAATSEPRKLEMLP
                     APTTNAAVATAPRIPALAPMADELAEPIGVRRTGSGAAPSGGPKLVAPEDAPVLLVTT
                     RPGALPSPGAPTRRADAGAPSSEGTPAPETADPNDPIAATSRNLQGYQRQLQGLLDNL
                     TKSASLATGQTTGQMRSGVLLGGPPTAGTAPIGAAPAGAGLFGGQLQGSSTPKVAASM
                     LGNRSLTLPKGTAFTCALKTTVISATSGLVGCQVQRNVFSDDGRVLLIERGSHLDGEY
                     RIASVRPGTVRIPVLWTRIRTPLGVTVDIDSPGTGQLGESGIDGYVDNRWGERIGAAM
                     LLSLIDDSVKLVIQNQASDRQADTIVLPSTTANTSKLAEKVLDSTINIPPLIYQNQGG
                     IVGIYVARDVDFSSVYELKPTATAPAREGTP"
     gene            6231..>6630
                     /locus_tag="PKV56_16220"
     CDS             6231..>6630
                     /locus_tag="PKV56_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOX69405.1"
                     /translation="MNQYACEHEDLGEGWCGDATSVVEFLRPLREQLDAPGVLEVCVN
                     RPGELLVETVRGWQTVPAPEMTQERCLSLATAVATFCDQQVNQERPLLSATLPSGERI
                     QFVIPPAVPRGTVSITVRKPSHLIKRLDDFE"
BASE COUNT         1148 a         2296 c         2138 g         1048 t
ORIGIN      
        1 tcgccgatgc ggtcaaggcc gtggcgatga tgccggcgcc gctgcgccgc ttctccaccg
       61 tgcgacagaa cctgcccaag gcgggcgaga acagtctctt cgagcgcctg ggccgctggt
      121 gccaaggtgg cgcgctcggc tgggtcttcg accaggccga cgaccgcctg ctcgacctgc
      181 acgccgcaaa cgtcattggc ttcgacacca ctgagttcct cgacctgccc gaggtccgca
      241 cgccggtgat gatgtacctg ctgcaggtga tggaagaact cgtcaacggc gagcggctga
      301 tctacgtgat ctcggagttc tggaaggccc tggaccacga gatcttcagc gacttcgcca
      361 agcagaagca gaagacgatc cgcaagcaga acggcctggg catcttcgac acgcagagcc
      421 cgtccgacgt actgcgccac ccgatcggcc gcacgatgat cgagcagagc gtgaccaaga
      481 tcttcctcgc caatccagag gcggtacgcg aggaatatgt ggacggcttc ggactgacgc
      541 aggccgagtt cgacatcgtg cgcagcctcg gcgcgcaggg cggccggcgc ttcctggtca
      601 agcagggtca cggcagcgcc atctgcgagc tggatctcgc cgggctcgag gacttcatca
      661 ccgtgctctc ggccaccacc gacaacgtgg ccctgctcga tgccgtgcgc gaacggcatg
      721 gcaacgatcc gtttcaatgg cttccagtgc tgctccgtga ggttcaggat cggaggtttc
      781 gtaccgctcg gagagccgca tgaatgctgt tcatcatctc ctcgcgctgg gcgcgctggc
      841 actcgccgcc tccaccgcaa gcgcccagtt cgtcaagggc aacgaggccg tcaaggtgat
      901 gcccgatgga acaaagaaag tcgagacacc tccgctgccc actactggcc ccattcgttc
      961 gacgaaacct tgcgcagcgg acgacaagtg ccacggcggc gcatggctga tggtggaaac
     1021 gaaggacggg ttggtcgagt gcaccgaagc gtacgcgcga cctggcacat gtcgcaagtc
     1081 aacctacggc gagaccaagc ttcttcgctt gtgggtcgtg agctcgggcg gaaagtggtt
     1141 gcagtgccag cgtccagaca cgtcgagcaa gtgcgtcgat atgttcgccc gcccacccgg
     1201 aaaccttcca ttcccggcaa ctcagtgaga ggacgtcgcc atgttcaact gggtcggact
     1261 gcagcttgat gggattctga gcacgtacgt gctcaatgtc gtgtcgacgc tcatggccgg
     1321 cctcgcgccc atagcgctca tctgcatgac gatctgggtg atgctctacg ggtgggccgt
     1381 gctacgcaac gaggtttcag agacgctgcc cgtcttcatg tggaaggcct tcaagatcgg
     1441 cctggtcttg gccttcgcat tgcagtccgg cttctacatc agcaacgttg ccgacacggc
     1501 aaatggcttg gccatgggcg tcgcgagcac gttcctcccc tctggtgtcg acccggtgac
     1561 ggtcacctcg ccgtatgcgc tgctggacaa gttcaacgac gacgccagcg cgcaagtcgc
     1621 cgacatcatg aaggaggcaa gcatgttccg cttggacttg ctcctcgcgg cggcgatctt
     1681 ctcgatcggt tctgtggtct tcctctgcat cgcgctcttc gtggtcacgc tctcgaagct
     1741 cttcttgacc ttcgtgatcg cgatcgggcc actgttcgtc ctgtgcctcg catggcgccc
     1801 cacagctcgc ttcttcgaca gttggctgtc gatggtgctg aacgctgtcg tgctgacctg
     1861 gttcgccttc ttcgctctcg gactgagtgc gtttatgggc gtgtcgatgt tcaaggcgat
     1921 acaggatggt ggtggctttc tcggtggcac gttcaacgtg ctgggcgagg ccacgcgcta
     1981 ctgcgttctg atgatcctca tggccatcat ctgcttccag gccccaagcc tcgcctctgc
     2041 gctcaccggc ggtgccgctg tccagcaggg tgtccagatg atccagaaca ccatgatggt
     2101 ctcgggtctt agatccggaa caggccgagg ctcgtcaggc aatccgacct ccggcggtgt
     2161 tgtgcgcgcc ggaaccggcc ccgcctacgc agccggtgcg tacgccgccg ccgccgggcg
     2221 agcagcacga acagcgacca gcggcgccag tggcgccgcc cgccagggct tcgcagccgc
     2281 ccggaccgct gcatacaagc ttgccgcgat gcgcggcagg acctgatcca ccgaacccga
     2341 caggagcacg tcatgaaact gcgcctcaag gccgcggccg ccgcgaccgc catcgtcctt
     2401 ggcaccggca acgcccaagc acagggcatc ccggtcatcg acatcgcgaa tctgatccag
     2461 accttgctgc aggtgttgaa cgacatcacc gagatcagca accaggttca gcagatctac
     2521 caactggaga accaggtcaa gagcctcaac ggcatccgaa acctcggcga cgtcttcaac
     2581 aacccgatgc tgcggaacta catcccggcc gaggcgtaca cccagctcaa tgcgatccag
     2641 aactcgggct atgccgcgct caacggcact gccaaggcgc tgcgggatgc gggcatggtc
     2701 tacaactgca tggacctcac cggcgcagca cgcaccacct gccagtcgac gctggcccag
     2761 ccgtaccagc acaaggggct gctgcaggac gccatgaagt cggcgtccgg acggctgtcg
     2821 cagatccagt cgctgatgaa ccagatcaac gcgaccaccg accagaaggc cgtgcaggag
     2881 atccaggccc gcatcggcgc cgagaacgcg ctgctggcgc acgaggtgtc ccaggtgcag
     2941 atgctgcagg gcatggccga cagcgaggaa cgcatcgcgc ggtcccgtga gcgcgagcgg
     3001 cagtacgaga tgcttgtccg caccgggaag gtggccgact acctgccctg atccctgaac
     3061 cgctgcgccg agccctcggc gcagccccac ggaggtccac catgagtgct gtcctgacgc
     3121 caggaaaagc cgctgcattg ccggatcgct cgcgccacac cggcaaggcg gctgccgagt
     3181 cgactgcgat cgacgccaac cgtgcgtggg aagtcgaccg cgcgctgatg ctggaacgct
     3241 cggaacgcag ggcttggtgg gtggccatcg cgggcctcgt tctggggctg atcggcatgg
     3301 cgggcgtgtt cgtgcagggt ccgctgcgcc gcgtggtgga gatcccgatc gtcgtcgacc
     3361 gtgtgaccgg cgaagcgacg atccagcagc gtctcgccgt ggagaccatc ccgccgatgg
     3421 aggccctcga caagcacaac ctcgccacct tcgtgcgggc ccgcgagggc tacagctgga
     3481 tgttcctgca gcgcgacttc gaccaggtgg cccgcatggc ggtgccggca gtgttcgccg
     3541 actacaaccg gcagttcgag ggtgacggcg cgctgcagaa gaagatcggc gccgccgagg
     3601 actggcgcat caacgtcatc ggcgtgcgcc tggcggcgtc cgggcgcgcc ggcaacaagg
     3661 gtgaggccac cgtcacctac gacaaggtgg tacgcctgac ggaccgcaac ctccccgagg
     3721 tcaccacgcg gcacgtggcc agcgtcgtct accagtacca acccaaggtg ctggccaagg
     3781 aacgcgatcg gctcgagaac ccgttcggct tcgtcgtcac ggcctaccgg tccgatcccg
     3841 agatcaacac ggccggccca ggagccaagc catgaagccg ctggcctggc tggctgcctt
     3901 cgcttgcgca gccccgtgcg tcatcgcgac ggccgcaccg gatgtcgccg acccgcgcct
     3961 gcgcgaggta gtctatgacc cgcgcgcggt ggtcaccgtg ccggtcaagc ggggcgtcgt
     4021 gacgctcgtg gtgctcgacc ccgacgagtc gatcaccgaa gtcgcagctg gccttggagg
     4081 agactgcacc aaggccgacg cggcctggtg cgtggccgcg cagcccggtg ggcgcaatct
     4141 gttcgtcaag gccaagagct cggccagagc ggcgaacaac ctggccgtgg tcaccgaccg
     4201 tcgcacgcac accttccgct tcgtggtcct ggccgatggc gatccgaagc cgccggtgta
     4261 ccggctcgtg gtcaagacac cgccgcgcca ggcgcctccg gcgcaactcg ccctgcgcga
     4321 ggacgtgcca ctcgtggcgc tgccggcggt cccgccgcct ccgccaccac agcaggtcgt
     4381 cgcggaacgc ctgcaggcga aaccgctggt gacgaacacg cagtactcga tcgccgaagg
     4441 cgccagctcg caggacatcg taccgacgct ggtctacgac gacggtcgct tcacgtacct
     4501 gcgtttcccc ggcaaccgcg aggtgccggc cgtgttccat gtgctgggcg acggcagcga
     4561 gacgctcgtc aacgcccgca tggaagacga cctgctggtg gtcgatcgcg tcagccgcaa
     4621 gttgatgctg cgcgccggcc aggctgtcgt cggcctctgg aacgaggcct tcgacttgga
     4681 cgggaggcca cccggcgacg gcaccacagt gcccggcgtg cagcgcgtcc tcaaggccga
     4741 tgccggtggc aacaacgcgc cgagcggcgc ccggaaggga gtgacaccat gacgcaagac
     4801 cctctcgacc aagagcccgg cgtggacgcc gatgcggcgg cggacggcaa ctcgatctcg
     4861 cctctgcccg gtgaacctgg catccccaac gtcgcggccc ggcagcgcgt ttcaacgtcg
     4921 aagaaagggc tacttgccgt ggccctgctg ctcggctccc tggtggccgt cgcggcgttc
     4981 acggtccagc gcgtctcggt cagcggcaag caggccgaag agccggagtc gaatctggtc
     5041 aaggacaagc ccaccgcggc cacgtccgaa ccccgcaagc tggagatgct gcccgcaccg
     5101 accaccaatg ccgccgtggc caccgcgccg cgcatcccgg cgctggcccc catggccgac
     5161 gagcttgcgg agccgatcgg tgtccgacgc accggatcgg gtgcagcgcc gtcaggtggc
     5221 ccgaagctgg tcgcgcccga agacgcacct gtcctgctgg tcaccacccg tcccggtgcc
     5281 ctcccgagcc ccggtgcccc cacccgtagg gcggatgccg gcgcaccctc ttcggagggc
     5341 actcctgcgc ctgagaccgc ggatccgaac gatccgatcg cggcgacctc gcgcaacctg
     5401 cagggctacc agcgccaact gcagggtttg ctcgacaacc tgacgaagtc cgcatccctg
     5461 gccacgggcc agaccaccgg gcagatgcgg tcgggggtcc tcctcggtgg gcccccgacg
     5521 gcaggcaccg caccgatcgg ggcggccccc gcaggggctg gcttgttcgg cggccagctt
     5581 caggggtcct ccacccccaa ggtggccgcc tcgatgctcg gcaaccgcag cctgacgctg
     5641 cccaagggca cggccttcac ctgcgcgctc aagaccacgg tgatcagcgc gacctccggc
     5701 cttgtcggct gccaggtgca gcggaacgtc ttcagcgacg acggccgggt gctgctgatc
     5761 gaacgcggct cgcacctgga tggcgagtac cggatcgctt cggtccggcc cggcacggtc
     5821 cgcatcccgg tgctctggac ccgcatccgc acgcccctgg gcgtcaccgt ggacatcgac
     5881 tctcccggaa ccggccagct cggcgaatcc ggcatcgacg gctacgtcga caaccgctgg
     5941 ggcgagcgca tcggcgcggc catgctgctg tcgctgatcg acgactcggt gaagctggtc
     6001 atccagaacc aggccagtga tcgccaggcc gacaccatcg tgctgccgtc gaccacggcc
     6061 aacaccagca agctggccga gaaggtactg gacagcacca tcaacatccc gccactgatc
     6121 taccagaacc agggcggcat cgtcggcatc tacgtcgccc gcgacgtgga cttctcgtcg
     6181 gtgtacgagc tgaagcccac cgccaccgca ccggctcgcg agggcacgcc atgaaccagt
     6241 acgcctgcga gcacgaagac ctgggcgaag gctggtgcgg cgatgccacg tcggtggtcg
     6301 agttcctgcg ccccctgcgc gagcaactcg acgcgcctgg cgtgctggaa gtctgcgtga
     6361 accggcccgg tgagctgctc gtcgagacgg tgcgcggatg gcagaccgta ccggcgcccg
     6421 agatgacgca ggaacgctgc ctgtcgctgg cgaccgccgt ggccaccttc tgcgaccagc
     6481 aggtgaacca ggagcgcccg ctgctgtcgg ccacgctgcc cagcggcgag cgcatccagt
     6541 tcgtcatccc gccggccgtg ccgcgcggca cggtatcgat cacggtgcgc aagccgtcgc
     6601 acctgatcaa gcggctggac gacttcgagc
//
LOCUS       DAOORV010000177         6596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14226_length_6596_cov_6.384956, whole genome shotgun sequence.
ACCESSION   DAOORV010000177 DAOORV010000000
VERSION     DAOORV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_6596_cov_6.384956"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3641)
                     /locus_tag="PKV56_16225"
     CDS             complement(<1..3641)
                     /locus_tag="PKV56_16225"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOX69406.1"
                     /translation="MNIIRLLISLVVGSVLNTAQALPSAIPPNIVATTQNKPMVMMTA
                     SKDFTMFWKAYTDFEDIDFDGKVDYTFKPAFSYYGYFDSAKCYTYSTANSGRFVPAVE
                     ATIVDGKYYCTAGSGYWSGNFLNWATMSRIDILRKVLYGGMRSFYGATAADRYANGDS
                     ATDTTLELSFVPRNSQAFVKYYNGSDLDRLTPFDSAAALAEGITLCRRPAENAGTSHS
                     SALIPQIRVVIGNYILWNMTEVRSCNWSTEVNYTWQAPTITFLSANYVSPNGVKATSD
                     ADYTHKASVPTAATNGATYSGGTLGPTFTGRVQACVADKLDPGCKQYGTVYKPTGLLH
                     EFGESSASGTKAARAEFALMMGSYDHNLEAGVLRKNMGEINDEINPATGQFKSAGVGK
                     GGIVQSLNEINLYGYDLSTGQYSQTCQSDTLTDGNCSSWGNPVGELLLESTRFFAGKA
                     AKWAAVGAKDLAVGMPAATNWVDPLSTASDDTLINGTKRSELYGRGICRPLNMLTISG
                     GVNSYDDVTAMLAFTDITNTATAEDLTKKIGDTEGITGTKRLVGNNGVDVADLQCTGK
                     TVSNLGKVQGACPDGPNFRGTYLGSGVAHFNNTNRIRGDLDAAAALIPDFPYAGLTVR
                     NYGVTMSGGVATIEVPLPGQVECSAANKGLANPDCKRVYITPASLDSQRGAPGPLPGN
                     MVDFKVLETTIDADGFASGSALVLWQHSMLGEDQDQDMLNSIRWVTGGTAAEPTLTIY
                     TQAVESNTGSNKAFGFGYTLVGTDKDGLHMHSGINKFLVADPTGVSVAAGAIYDGADD
                     NTQCLASGTVNEPKKLCSKLGAAGNVVRGETYKTFNMTGATNASLKEPLWYIAKYGGF
                     SYTKNDKTAAATPVFTPTRYNQWDRRKSDGTICNDPTQPCDGNPDNYFYARRPDLLEK
                     SLREILEDIVTSSNTAPAISASQLQANDLKVVASFDAADGSGALTAYALKAETGAFEV
                     DPDFTWRAHERLTATLSTNRQIITNRVGADGVPFEWASLDGTEQDILKEGSTVAHGQK
                     MLDWIRGSTSFADEFRPRPATSIMGPIVNSNPTIQTAPEGRYFGDAFSGYDTFATTWK
                     DRRLVLWAGAGDGMLHAFDASNRKGPDGSQAGGAPIMSYIPQPLFSRLPDWASANGAK
                     VQAFVDGSPFVGDVKVGADWRTYLFSSLGRGGKGIFALDVTQAGTITTDTKGTETTAD
                     DTTSVVGSQL"
     gene            complement(3653..4312)
                     /locus_tag="PKV56_16230"
     CDS             complement(3653..4312)
                     /locus_tag="PKV56_16230"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69407.1"
                     /translation="MHVTPVIATPPLTTRSGSRGIALPIVLMFLLIITIAAAFGIRRA
                     TLGEGITRNQLDYEVARQAAEAALRDAERDLFPMFAGKPAGAVCDRSPDRPLPTKFIP
                     SLWTTDCRKGQCLSPPAYLAASNYATGVNPQPWWPASKDGLWGDNEAIPVGCDFDGGI
                     PIGTFTGTPNINGVARQPEYMLEWIDRATDTLIRVTARGFGADINTEVVLQTYVKPPG
                     K"
     gene            complement(4324..5361)
                     /locus_tag="PKV56_16235"
     CDS             complement(4324..5361)
                     /locus_tag="PKV56_16235"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HOX69408.1"
                     /translation="MNTMQRQLTALDKKSGRASRQRGMTLVELMVGLVIGIVLSLAAA
                     ALYLATREASRTTQSISDINETGKIALEMIGREVQKAGFYPAQYGQDTTTADQFYGVY
                     YNAKDATKVAFNTGLFGCAGANYNPATKACGPTVAGQSDSIIVNYFATPEFGADSLLG
                     NANDCNRQPVSGDAVNAASVAASQPLYVSNRFGIVDTDSYVDSDKNTVTVNSLGCHGN
                     GDEAGSAQPALEGIEDMVIRYGIYAGDGDAAAAGKSPTSFMTATEVNEVDLTGPVGGR
                     TAWQRVTAVSICIVVRSLANSRQEDKAGEVRTYRNCRDADPVELPAGSRYIYKSFERV
                     YAVRNNLSDNL"
     gene            complement(5358..6023)
                     /gene="pilV"
                     /locus_tag="PKV56_16240"
     CDS             complement(5358..6023)
                     /gene="pilV"
                     /locus_tag="PKV56_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HOX69409.1"
                     /translation="MNTIEQTPSPASGCRVESVPIGQRRRGETGSTLIEALVAMLVLS
                     IGLLGIAGLASAALRYSQGAWARAAVASGLSDLADRVRANPSASNTDYRFNTVAYEAQ
                     REALEDGDVTIARNCLAEDCTPAQLATYHLTDWQLALDRSMPGAAVWVSGQRDTGYLA
                     TIMWFDKSYVQDDGVTPEASPTCAVDGAGVITDTGIAARICCPPAADVIDGVRCTNMS
                     VIP"
     gene            complement(6028..>6596)
                     /locus_tag="PKV56_16245"
     CDS             complement(6028..>6596)
                     /locus_tag="PKV56_16245"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOX69410.1"
                     /translation="RHLQRGFTLIEMMTVVSLIALIAALAAPDLRAFMVRNKVAGVGN
                     EFAAALQQARALAVSRNSCVSLCAASSNNTATCAENDVEDLNLLAAGWLIFQNPGCDA
                     TKTDPAADAAGPVLQQRSGESGNYGLVPSDPLLNIVLFDPRGYANLAASGNFQVQPPA
                     GTDDSYRRTVCLDAAGRATVRQYTDSCT"
BASE COUNT         1054 a         2034 c         2208 g         1300 t
ORIGIN      
        1 agttgggagc cgaccacgct ggtcgtgtca tcggcggtgg tctcggtgcc tttggtgtcg
       61 gtggtgatcg tgcccgcctg ggtcacgtca agcgcaaaga tgcccttgcc gccccggccc
      121 aaagacgaga agagataggt gcgccagtcg gcacccacct tcacgtctcc gacgaatggc
      181 gagccgtcga cgaaagcctg gaccttcgcg ccattggccg aagcccagtc aggcagtcgc
      241 gagaacaagg gctgcgggat gtacgacatg atcggggcgc caccggcctg cgaaccgtca
      301 ggtcccttgc ggttgctggc gtcgaaggcg tgcagcatgc cgtcgccggc gccggcccac
      361 agcaccagtc gccgatcctt ccaggtcgtg gcgaaggtgt cgtatccgct gaaggcatcg
      421 ccgaaatagc ggccctcggg agcggtctgg atcgtcgggt tcgagttgac gatcggcccc
      481 atgatcgacg tggccgggcg aggcctgaat tcatcggcaa acgaggtgct gccgcggatc
      541 cagtccagca tcttctgccc gtgagccacg gtgcttccct ccttcaggat gtcctgttcg
      601 gtgccatcca ggctggccca ttcgaacggg acgccgtcag ccccgacccg gttcgtgatg
      661 atctggcggt tggtcgacag ggtggcggtg agccgctcgt gggctctcca ggtgaagtcc
      721 ggatccacct cgaacgcacc cgtctcggcc ttcagcgcgt acgccgtcaa cgcaccgctg
      781 ccgtcggctg catcgaagga agccaccacc ttcaagtcgt tcgcctgcaa ctggctcgcg
      841 gagatggccg gagccgtgtt ggacgaggtc acgatgtctt cgaggatctc gcgcagcgac
      901 ttctccagca ggtcaggtcg acgcgcgtag aaatagttgt ccggattgcc gtcgcagggt
      961 tgcgtcgggt cattgcagat ggtcccgtcg gacttgcgac ggtcccactg gttgtagcgg
     1021 gtcggcgtga agacgggcgt ggcggcagcc gtcttgtcgt tcttcgtgta gctgaaaccg
     1081 ccgtacttgg cgatgtacca gagtggctcc ttcaggctgg cattggtggc gccggtcatg
     1141 ttgaacgtct tgtaggtctc gccgcgaacc acgttgccgg ctgcgccgag cttggagcac
     1201 agcttcttgg gctcgttcac cgtgccgctg gccaggcact gcgtgttgtc gtcggcgccg
     1261 tcgtagatcg ctcccgcagc cacactcacc cccgtggggt cggcgaccag gaacttgttg
     1321 atgccgctgt gcatgtgcag gccgtccttg tcggtcccca ccagcgtgta tccgaagcca
     1381 aaagccttgt tcgaaccggt gttcgattcc actgcctggg tgtagatcgt cagcgtcggt
     1441 tcggcggccg ttccacccgt cacccagcgg atcgaattca acatgtcctg gtcctggtct
     1501 tcacccagca tgctgtgctg ccacagcacc agcgcggagc ccgacgcgaa gccgtccgca
     1561 tcgatcgtcg tttccagcac cttgaagtcg accatgtttc ccggcagcgg accgggtgcg
     1621 ccgcgctgcg aatccaggct ggccggcgtg atgtagaccc gcttgcagtc cggattcgcc
     1681 aggcccttgt tggcagccga gcactcgacc tggcccggca acgggacctc gatggtggca
     1741 acgccccccg acatcgtgac gccgtagttg cgcacggtca gcccggcata cgggaagtcc
     1801 ggaatcaggg cggccgccgc atccaggtcg ccacggatac ggttcgtgtt gttgaagtgg
     1861 gccacgcccg agccgaggta ggtcccccgg aaattgggcc cgtccggaca ggcgccctgc
     1921 accttgccca ggttggaaac cgtcttgccc gtgcattgca gatcggcaac gtccacgccg
     1981 ttgttgccga ccagccgctt cgtgccggtg atgccttcgg tgtcaccgat cttctttgtc
     2041 agatcctccg ccgtggcggt gttggtgatg tcggtgaacg caagcatggc tgtcacgtcg
     2101 tcgtagctgt tgacgccgcc cgagatcgtc agcatgttca gcgggcggca gatgccacgt
     2161 ccgtagagtt cagatctctt cgtgccgttg atcagggtgt cgtccgaggc cgtgctcagc
     2221 gggtcaaccc agttcgtggc ggcgggcatg cctaccgcaa ggtccttggc tccaacggcc
     2281 gcccacttgg ccgccttgcc ggcgaagaaa cgcgtcgatt cgagcagcag ttcacccacc
     2341 gggttgcccc aggaagagca gttgccatcg gtcagcgtgt cggattgaca ggtctgcgaa
     2401 tactgtccgg tgctcaggtc gtatccgtag aggttgattt cattcagcga ctgaacgatc
     2461 ccgcccttgc cgacccccgc actcttgaac tggccggtcg ccgggttgat ctcgtcgttg
     2521 atctcgccca tgttcttgcg cagcaccccc gcttcgaggt tgtggtcgta cgagcccatc
     2581 atcagggcga actcggcgcg cgcggccttg gtgccgctgg cgctgctttc gccgaactca
     2641 tgcagcagac cggtgggctt gtagaccgtg ccgtattgct tgcagccggg atcgagcttg
     2701 tcggccacgc aggcctgaac ccgtccggtg aaggtcggcc ccagagtccc accgctgtag
     2761 gtcgcgccgt tggtcgccgc cgtcggcaca ctggccttgt gcgtgtaatc ggcgtcggag
     2821 gtggccttga cgccattcgg gctgacgtag ttggcgctca ggaacgtgat cgtcggcgcc
     2881 tgccaggtgt agttgacctc cgtggaccag ttgcacgacc tcacctcggt catgttccag
     2941 aggatgtagt tcccgatcac cacccggatc tgcggtatca atgcgctgga gtgggaggtc
     3001 ccggcgtttt ccgcaggacg acggcacaac gtgatgccct cggccagcgc tgccgcgctg
     3061 tcgaacggcg tcaggcggtc gaggtcgctg ccgttgtaat acttgacgaa agcctggctg
     3121 ttgcgcggca cgaacgagag ctcgagcgtc gtgtcggttg cggagtcgcc attggcgtag
     3181 cgatcggctg cggtggcgcc gtagaacgac cgcatgccgc cgtagagcac cttgcgcagg
     3241 atgtcgatgc gcgacatcgt ggcccagttc aggaagttgc cgctccagta gccgctgcca
     3301 gccgtgcagt agtacttgcc gtcaacgatg gtcgcttcga ccgcgggaac gaaccgcccg
     3361 ctgttggcgg tgctgtaggt gtagcacttc gctgaatcga agtacccgta gtagctgaac
     3421 gcgggcttga aggtgtagtc gaccttgcca tcgaagtcga tgtcctcgaa gtcggtgtag
     3481 gccttccaga acatcgtgaa gtccttggag gccgtcatca tcaccatcgg cttgttctgc
     3541 gtggtggcga cgatgttcgg cgggatggcg ctgggcaggg cctgggccgt gttcagcaca
     3601 cttccgacga caagggatat gaggaggcga atgatgttca tggcagctcg tttcatttgc
     3661 cgggaggctt gacgtaggtt tgcagaacca cttcggtgtt gatgtcggcg ccgaagccac
     3721 gtgccgtgac gcggatcagc gtgtcggtgg cccggtcgat ccactcgagc atgtactcgg
     3781 gctggcgcgc aacgccattg atgttcgggg tgccggtgaa ggtgccgatc gggatgccgc
     3841 catcaaagtc gcaaccgacg ggtatcgctt cgttgtcgcc ccacaggcca tccttgctcg
     3901 ccggccacca gggttggggg ttgacgccgg tagcgtagtt gcttgccgcg aggtaggccg
     3961 ggggggaaag gcactggccc ttgcggcagt cggtcgtcca caggctgggg atgaatttgg
     4021 tgggcaaggg gcgatccggg ctgcgatcgc agacggctcc ggccggcttg cctgcgaaca
     4081 tcggaaacag gtcccgttcg gcgtcgcgca gggccgcttc agcggcctgg cgggcgactt
     4141 cgtagtcgag ctggttgcgc gtgatgcctt cgcccagcgt ggcgcgtcgg atgccgaacg
     4201 cagccgcgat cgtgatgatc agcaggaaca tcagcacgat gggaagtgcg atgccccgcg
     4261 atcccgagcg agtcgtgagc ggcggggttg cgatgacagg tgtcacgtgc atggcaggcc
     4321 ggttcacagg ttgtcgctca ggttgtttcg aacggcatac acgcgctcga aactcttgta
     4381 gatgtaccgg ctgccggcgg gcagctcgac gggatcggca tcgcggcagt tgcgatacgt
     4441 gcgcacctct cccgccttgt cttcctggcg tgaattggcc agcgaacgca ccacgatgca
     4501 gatgctgacc gccgtgacgc gttgccacgc cgtgcgtccg ccgaccgggc cggtcaagtc
     4561 gacctcgttg acctcggtcg ccgtcatgaa tgacgtgggt gacttgcctg cggcagcagc
     4621 atcgccatcg ccggcataga tgccgtagcg aatgaccatg tcctcgatgc cttcgagtgc
     4681 cggttgcgcg ctgcccgcct cgtcgccatt gccgtggcaa cccaggctgt tcacggtgac
     4741 cgtgttcttg tcggagtcga catagctgtc ggtgtcaacg atgccgaacc ggttcgagac
     4801 atacagcggc tggctggctg caacgctggc tgcattgacc gcgtcgccgg acaccggctg
     4861 acgattgcag tcattcgcgt tgccgagcag cgagtcggcg ccgaattcgg gtgtagcgaa
     4921 gtagttgacg atgatcgagt cggactgccc cgccaccgtt gggccgcaag ccttggtggc
     4981 cgggttgtag ttggctccgg cgcagccgaa gagtcccgtg ttgaaagcga ccttggtcgc
     5041 gtccttggcg ttgtagtaga cgccgtagaa ctgatcagct gtcgtggtgt cctgcccgta
     5101 ctgagccgga tagaagccgg ccttctgcac ttcacggccg atcatctcca gcgcgatctt
     5161 cccggtctcg ttgatgtccg agatggattg cgtggttcga gaagcctcgc gtgttgccag
     5221 gtagagcgcg gcagccgcca gcgacagcac gataccgatg accaggccga ccatcagttc
     5281 gaccagggtc atgccgcgtt gccgggaggc ccgccccgac ttcttgtcca gggctgtcag
     5341 ttgtcgttgc atcgtgttca tggaatcacc gacatgttgg tgcagcgcac gccgtcgatc
     5401 acatcggctg caggcgggca gcagatccgt gcggcgatgc cggtgtcggt gatcacgccc
     5461 gcgccgtcga ccgcgcaggt cggggaggct tccggtgtga cgccgtcgtc ctggacgtag
     5521 gacttgtcga accacatgat cgtggccagg tagccggtgt cgcgctgacc ggacacccag
     5581 actgccgcgc ccggcatgct gcggtcaagc gccaactgcc aatcggtcag gtgataggtc
     5641 gccaactggg ctggcgtgca gtcctcggca aggcagttgc gggcgatggt cacgtcgccg
     5701 tcttcaagcg cttcgcgttg agcctcgtag gcgacggtgt tgaacctgta atcggtgttc
     5761 gaggctgacg gattggcgcg aacgcggtct gcgagatccg acaggcctga agccaccgca
     5821 gcgcgagccc aggcgccttg cgagtaacgc aacgccgcgg atgcgaggcc tgcgatgccc
     5881 agcaagccga tggacaacac cagcatcgcg accagcgcct cgatgagtgt gctgcctgtt
     5941 tcgcctcggc gccgctgtcc gatcggaacc gattcgacac ggcagccgga ggccggggat
     6001 ggggtctgct cgatcgtgtt catgggctca ggtgcatgag tcggtgtatt gccgcacggt
     6061 ggctcggccc gcggcgtcca ggcaaacggt gcggcggtaa ctgtcatcgg tgcctgccgg
     6121 tggctggacc tggaagttgc cgctcgcagc cagattggcg tatccgcgcg ggtcgaacag
     6181 cacgatgttc agcagagggt cggacggaac gaggccgtaa tttccggact cgccggaacg
     6241 ctgttgcagc accgggccgg ctgcatcggc cgccgggtcg gtcttcgtgg catcgcaccc
     6301 cgggttctgg aagatcagcc agcctgcagc gaggagattc aggtcttcga catcgttctc
     6361 cgcgcaagtc gccgtgttgt tgctcgatgc cgcgcacagg gacacgcagc tgttgcgcga
     6421 aacggccagt gcccgcgcct gctgcagtgc tgccgcaaac tcgttgccga cgccggccac
     6481 cttgttgcgg accatgaacg cgcgcagatc cggggccgcg agcgccgcaa tgagggcgat
     6541 cagcgagacc acggtcatca tctcgatcag cgtgaacccg cgctgcaggt gtctca
//
LOCUS       DAOORV010000178         6592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14231_length_6592_cov_5.213401, whole genome shotgun sequence.
ACCESSION   DAOORV010000178 DAOORV010000000
VERSION     DAOORV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14231_length_6592_cov_5.213401"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKV56_16250"
     CDS             <1..648
                     /locus_tag="PKV56_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOX69411.1"
                     /translation="IQVDNPSKMPDLGRINEIREPIVTVHLYMPQDYVGPVMTLANLK
                     RGNQLNMAYHGRQVMLTYEMPLAEIVLDFFDKLKSVSRGYASMDYEFKEYRAADVVKV
                     DILLNGDKVDALSIIVHRSQSQFRSRAVVAKMRELISRQQYDVAIQAAIGANIIARET
                     IKALRKNVIAKCYGGDVSRKRKLLDKQKAGKKRMKQIGSVEVPQEAFLAILQVED"
     gene            648..1613
                     /gene="lepB"
                     /locus_tag="PKV56_16255"
     CDS             648..1613
                     /gene="lepB"
                     /locus_tag="PKV56_16255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOX69412.1"
                     /translation="MSTLTGLLYGCLIVFLGGWYLGEWSGNFSLLLFILTMVTMAYWL
                     VERLYFAPRRIAAAQALEQQAEARRRQLAEQGIAKVDGDVGQAREALLAQPWWLDWTA
                     GLFPVILVVFLLRSFLFEPFKIPSGSMIPTLLVGDLILVNKFHYGIRLPVINKKIIPN
                     HDPVRGDVMVFRYPANPSIDYIKRVVGVPGDEVSYRHQQLYLNGALVPSQPLPDFYNE
                     DTLRYANQFDETLGALSHRILVDPARSTYLRPSMVFPFSENCTYIVDGVTCKVPVGHY
                     FMMGDNRDNSEDSRFWGFVPDANIVGRAFFVWMNFGNVRRIGSFH"
     gene            1682..2053
                     /locus_tag="PKV56_16260"
     CDS             1682..2053
                     /locus_tag="PKV56_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOX69413.1"
                     /translation="MTSKLHPARSDQRGVTLLGLLLWAIIIGFLALLGMRVLPTVNEF
                     FTIQKAVNKVALEGGTTVPEIRAAFEKQKDIEYSIQSISAKDLDITKENDKITIRFAY
                     DKEVEVMAPVFLLIKYEGRSR"
     gene            2089..2793
                     /gene="rnc"
                     /locus_tag="PKV56_16265"
     CDS             2089..2793
                     /gene="rnc"
                     /locus_tag="PKV56_16265"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOX69414.1"
                     /translation="MDPRLDALQRRLGHRFSDVGLLALALTHRSFSAEHNERLEFLGD
                     AVLSLAISSLLYERFAGSDEGDLTRVRAHLVREDSLHKVAVLQGLPEVLRLSDGEARG
                     GGAQRASILADALEAIIGATFLDGGFASARALVQRLFGDVINTTEIGGWAKDAKTELQ
                     EWLQARRLPVPGYRISATRGQAHAQTFEVECEVPALSLKHLGEGRSRRLAEQEAARRM
                     LDALKANDQPGSPLRS"
     gene            2846..3775
                     /gene="era"
                     /locus_tag="PKV56_16270"
     CDS             2846..3775
                     /gene="era"
                     /locus_tag="PKV56_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOX69415.1"
                     /translation="MTESAETPAASDPASQRCGLVAIVGRPNVGKSTLLNALVGQKVS
                     ITSAKAQTTRHRITGIRTAGDSQFVFVDTPGFQTRYAAAMNRTLNKTVHSVLADVDVV
                     LFVVEAGRFGLDDAKVLALMPSGEYAKPVFLIANKLDAVHRRAELAPWLKGMQDRHAF
                     AEFVPLTAKKDADVQRLLKIIEPYLPQQPWFYEEDALTDRSDKFLASEIIREKLFRLM
                     GDELPYTSTVVIDRFEEEGNLRRIAASIVVEREAHKGMVIGGNGERLKRIGSEARQEL
                     EKLMGATVFLELWVKVRSGWADDEEHLRSYGYE"
     gene            3813..4643
                     /locus_tag="PKV56_16275"
     CDS             3813..4643
                     /locus_tag="PKV56_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOX69416.1"
                     /translation="MLTAFVLHRYDWSESSLILDVFTREQGRLVVAAKGAKRPYSQLR
                     SVLLPFQRLHLSLGRIPKTEPSEGQQGDSPVREVQTLRGAEWAGGAAMLTGAALFSGF
                     YLNELLMKLLPRQDAHPLLFDVYAQTLTALAGRDETRVQSALRAFEITLLQQTGVLPD
                     LSLVTSTLDAVHPDGRYALLPEAGVAFARADEPSVSGSALIDVQAALLHGSLAPVQQA
                     CARALSDWRSLLRGLLHYHLGTPALRTRQVMLDLQSLSSSPVNTSPQKQGSAPVAGRP
                     "
     gene            4661..5464
                     /locus_tag="PKV56_16280"
     CDS             4661..5464
                     /locus_tag="PKV56_16280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOX69417.1"
                     /translation="MNPLLTTGLDAHRGHTALSVNLNKVALLRNQRPLDIPSVTRAAT
                     LALQAGAGGITVHPRPDERHIRAHDVFDLAQLMRDWPQAEYNIEGNPLHNLMDVIREV
                     TRRGLPLHQCTFVPDAMDQATSDHGWRFPEDADALAPLIAEAQALGVRVSLFMDPVPQ
                     AMRAVRELSAERVELYTESYARAHGSPDEAAVLAGYTATADAALVEGLQLNAGHDLNR
                     NNLTDFLRAVPGVREVSIGHALIADALELGISETVRDYLRCLHRASAPA"
     gene            5461..5856
                     /gene="acpS"
                     /locus_tag="PKV56_16285"
     CDS             5461..5856
                     /gene="acpS"
                     /locus_tag="PKV56_16285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOX69418.1"
                     /translation="MIYGVGTDICDLRRIAATLERRGERFAEKVLGPQEMQVFLARRL
                     RVQARGVRYLGTRFSAKEAFSKAIGLGLRMPMTWRACEILNLPSGQPHVVLHGELAAW
                     FEARQLKAHVSVTDETDYATSFVVVESCP"
     gene            5853..>6592
                     /gene="nagZ"
                     /locus_tag="PKV56_16290"
     CDS             5853..>6592
                     /gene="nagZ"
                     /locus_tag="PKV56_16290"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOX69419.1"
                     /translation="MKTTRTTARRRLSKQVPGAETPHAPVVIDIDGLCLDADDRRRLR
                     HPLTGGLILFARNWQDRHQLTELTAEIKSIRPDVLICVDHEGGRVQRFRTDGFTHLPP
                     MRVLGEQWMTDPMGATDSATASGFVLAAELRACGVDLSFTPVLDLDFGSSGVIGDRAF
                     HRDPRVVTLLAKSLMHGLLRAGMSHCGKHFPGHGHVKADSHTEIPVDKRSLKAILADD
                     ARPYEWLSTSLASLMPAHVVYPKVDANPA"
BASE COUNT         1177 a         2075 c         2100 g         1240 t
ORIGIN      
        1 atccaggtcg acaacccgtc gaagatgccc gacctcggcc ggatcaacga gatccgggaa
       61 cccatcgtga ccgtgcatct gtacatgccg caggactacg tggggccggt gatgacgctg
      121 gccaatctga agcgcggcaa ccagctcaac atggcatatc acggccggca ggtcatgctg
      181 acctacgaga tgccgctcgc ggagatcgtg ctcgacttct tcgacaagct gaagagcgtt
      241 tcacgcggct acgcctcgat ggattacgag ttcaaggagt accgcgcggc cgacgtcgtc
      301 aaggtcgaca tcctgctcaa cggggacaag gtcgatgccc tgtcgatcat cgtgcatcgc
      361 agccagagcc agttccgcag cagagcggtg gtcgccaaga tgcgcgagct gatttcccgg
      421 cagcagtacg acgttgccat ccaggccgcc atcggggcca acatcatcgc gcgtgagaca
      481 atcaaggcgc ttcgaaagaa cgtgatcgcc aagtgctatg gaggcgatgt ctcgcgcaaa
      541 cgcaagctgc tcgacaagca gaaagccggc aagaagcgca tgaagcagat cggatcggtg
      601 gaggtgccgc aggaggcatt tctggccatt ctccaagtgg aagattgatg agtacgttga
      661 ccggtctgct ctacggttgc ctgattgttt ttctgggcgg ctggtatctc ggcgagtgga
      721 gtggcaattt ctcgctgctg ctgttcatcc tgaccatggt gacgatggcg tactggctgg
      781 tcgagcggct gtatttcgct ccgcggcgaa tcgccgccgc ccaggcgctg gagcagcaag
      841 ctgaagcccg tcgccggcaa ctcgccgagc agggcattgc gaaggtggac ggcgatgtcg
      901 gccaggcgcg cgaggcgctg cttgcccagc cgtggtggct ggactggacg gccgggctgt
      961 ttcccgtgat cctggtggtg ttcctgctgc gctcgtttct tttcgagccg ttcaagatcc
     1021 cgtcgggctc gatgattccg accttgctcg tcggcgacct gattttggtg aacaagtttc
     1081 actacggcat caggctgccg gttatcaaca agaagatcat tcccaatcac gatcccgtgc
     1141 gcggcgacgt gatggtgttc cggtatccgg ccaacccgag catcgattac atcaagcgcg
     1201 tggtcggtgt gcctggtgac gaggtgtcgt atcggcatca gcagctctac ctgaacggcg
     1261 cgctggttcc aagtcaaccc ttgcccgact tctacaacga ggacacgctg cgttacgcga
     1321 accagttcga cgagacgctg ggagcgctgt cgcatcgcat cctggtcgat ccagcccgtt
     1381 caacttatct gcgtccgtcc atggtgttcc cattcagcga gaactgcacg tacatcgtcg
     1441 acggcgtgac ctgcaaggtg ccggtgggtc actatttcat gatgggcgac aaccgcgaca
     1501 attccgagga ttcgcgcttc tggggcttcg tgccggacgc gaatatcgtc ggccgcgcct
     1561 tcttcgtctg gatgaatttc ggcaatgtgc ggcgaatcgg atcgtttcat tgatccatcc
     1621 tcgcggaaaa tgtgcgctgg cgcagccgtc cccttctttc agagcactgg agcgtgaacg
     1681 catgactagc aagcttcatc cggcccggag tgaccagcga ggcgtcacct tgctcggcct
     1741 gttgctgtgg gcaatcatca tcggcttcct ggcactgttg ggaatgcgtg tactgccaac
     1801 ggtgaatgaa ttcttcacca tccagaaagc cgtcaacaag gtcgcccttg agggcggcac
     1861 cacggttccc gagatccgcg cggccttcga gaagcaaaag gatatcgaat actcgattca
     1921 gtcgatctcc gccaaggacc tcgacatcac caaagagaac gacaagatca ccatccgatt
     1981 cgcctacgac aaggaggtcg aggtgatggc cccggtattt ctgttgatca agtacgaggg
     2041 caggtctcgc taggccgcct ggcgttcgcg gtcgaccgca tcgtctcgat ggacccacgc
     2101 ctcgacgccc tccagcgccg cctcgggcac cgtttttccg acgtcggatt gctggcgttg
     2161 gccctgactc accgcagctt cagcgccgag cacaacgaac gcctggaatt cctcggcgat
     2221 gcggtgctga gtctggcgat atcgagcctg ctctacgagc gtttcgcggg atcggacgag
     2281 ggtgatctga cccgcgtgcg tgcccatctc gtgcgtgagg acagccttca caaggttgcg
     2341 gtgctgcagg gcttgcccga ggtgttgcgg ctcagcgatg gcgaggcgcg cggtggtgga
     2401 gcccagcggg cgtcgatcct ggccgacgcg ctggaggcca tcattggtgc cacattcctc
     2461 gatggcggtt tcgcgtcggc gcgggcgctg gtgcagcgcc tgttcggtga tgtgatcaac
     2521 accaccgaga tcggtggatg ggccaaggac gcgaaaaccg aattgcagga atggctgcag
     2581 gcgcggcgtt tgcccgtacc cggttatcgc atcagcgcga cccgcgggca ggcgcatgcg
     2641 cagaccttcg aggtcgaatg cgaggtgcct gccctcagcc tgaagcacct gggcgagggc
     2701 cgctcgcgtc gccttgccga gcaggaagcc gcgcgccgga tgctcgatgc cctgaaggca
     2761 aacgaccagc cgggcagccc gttgcggtcc tgaatcctta ttccgctcga attgccgacc
     2821 tgaggttttc caatgattgc agtccatgac cgaatccgct gagacgcctg ccgccagtga
     2881 tcccgcttct cagcgttgcg ggctggtggc catcgtcggt cgcccgaacg tcggcaagag
     2941 cacgctgctc aacgcgctgg tcggacagaa ggtcagcatc acctcggcca aggcccagac
     3001 cacacggcat cgcatcaccg gcattcgcac cgccggggac tcgcagttcg tcttcgttga
     3061 cacgccgggt ttccagaccc gctacgccgc ggcgatgaac cggaccctca acaagaccgt
     3121 gcacagcgtg ctggccgacg tggacgtggt gctgttcgtc gtcgaggccg gacgtttcgg
     3181 cctggatgac gcgaaggtgc ttgcgctgat gccgtcaggc gagtacgcga agccggtgtt
     3241 cctgatcgcc aacaagctgg acgctgtgca ccgccgggcc gaactggcgc catggctcaa
     3301 gggcatgcag gaccgccatg cgtttgccga gttcgtgccg ctgacggcga agaaggacgc
     3361 tgacgtgcag cgtttgctga agatcatcga gccctacctg cctcagcagc cctggttcta
     3421 cgaggaggat gcgctgaccg atcgcagcga caagttcctg gccagcgaga tcatccgcga
     3481 gaagctgttt cgtctgatgg gagatgagtt gccctacacc tcgacggtgg tcatcgacag
     3541 gttcgaggaa gagggcaatc tgcgacgcat tgcggccagc atcgtcgtcg agcgcgaggc
     3601 gcacaagggc atggtcatcg gcggcaacgg tgaacgcctg aagcgcattg gcagcgaagc
     3661 acgacaggaa ctcgagaagc tgatgggcgc cacggtgttc ctcgagcttt gggtcaaggt
     3721 gcgctccggc tgggcggatg acgaggaaca cctgcgcagc tacggctacg agtgagcgag
     3781 tggtcacgcg ccggccatcc aaggcgccgt cggtgctgac ggccttcgtg ctgcaccgct
     3841 acgactggag cgaatcgagc ctgatccttg acgtcttcac gcgtgaacag gggcggttgg
     3901 tcgtcgctgc gaaaggtgcc aagcggccct actcccagtt gcgttcggtg ctgctgccgt
     3961 tccagcggct gcatctgtcg ctcgggcgaa ttccgaagac agagccttct gaagggcagc
     4021 aaggcgacag tccggtgcga gaagtgcaaa ccctccgggg tgccgagtgg gccgggggcg
     4081 cagcgatgct gaccggtgca gcgctgttca gtggcttcta cctgaacgaa ttgctgatga
     4141 agctcctccc caggcaggat gcgcacccgc tgctcttcga cgtctatgcg cagaccctga
     4201 ccgcactcgc aggtcgcgat gagactcggg tgcaatcggc cttgcgcgca ttcgaaatca
     4261 cactgctgca gcagacaggc gtgctgcccg acttgagtct ggtcacgtcg acgcttgatg
     4321 cggtgcatcc cgacggtcgc tacgcgttgt tgccggaagc cggcgttgca ttcgcgcgag
     4381 cggacgagcc gtcggtatcg ggttccgcct tgatcgatgt gcaggcggca ctgttgcacg
     4441 gcagcctggc gccggtgcag caggcgtgcg cccgggccct gtccgattgg cggagtctgc
     4501 tgcgcggtct gcttcactat catctgggca ccccggccct gcgcacgcga caggtgatgc
     4561 tggacctcca atccctctcc tcgtcgcccg tgaacacgtc ccctcagaag cagggctctg
     4621 cgccagtcgc aggcaggcct tgatccgact cccgaccacc atgaatcccc tgctcaccac
     4681 cggcctcgat gcgcatcgcg ggcataccgc gctctcggtc aatctgaaca aggtggcgtt
     4741 gctgcgcaac cagcgcccgc tcgacattcc cagcgtcacc cgtgccgcca cgctggccct
     4801 gcaagccggc gcgggtggca tcaccgtgca cccgcgtccc gacgagcgcc acatccgcgc
     4861 tcacgacgtc ttcgatctgg ctcagctgat gagagattgg ccacaggccg aatacaacat
     4921 cgaggggaat ccgttgcaca acctgatgga tgtcattcgc gaagtgactc ggcgtggtct
     4981 gcccctgcac cagtgcacct tcgttcccga tgcgatggat caggccacat ccgaccatgg
     5041 ctggcgcttt cccgaggacg ccgatgcgct ggcgccgctc atcgccgagg cgcaggcact
     5101 cggcgtgcgg gtcagcctgt tcatggaccc cgtgccacag gcgatgcgcg cggtgcgcga
     5161 gttgtccgcc gaacgcgtgg agctctacac cgagagttac gcgcgtgcgc acggatcgcc
     5221 agacgaggcg gccgttctgg ccggctacac cgccactgcc gatgctgcac tggtcgaggg
     5281 cctgcagctc aatgccgggc atgacctgaa ccgcaacaac ctgaccgact tcctgcgcgc
     5341 cgtgcccgga gtgcgggagg tgtcgatcgg ccacgcattg atcgccgatg cgctggaact
     5401 gggcatcagc gaaaccgtgc gcgactacct gcgctgcttg caccgggcca gcgctccggc
     5461 atgatctacg gggtcggaac cgacatctgc gacctgcgcc gcatcgcagc cacgctcgag
     5521 cgtcgcggcg agcgtttcgc cgagaaggtg ctgggtccgc aggagatgca ggtgtttctt
     5581 gcgaggcgcc tgcgcgtcca ggcgcgcgga gtccgctacc tgggcacccg tttttctgcg
     5641 aaagaagcct tttccaaggc catcggcctg gggctgcgca tgccgatgac ctggcgcgcc
     5701 tgcgaaatcc tgaatctccc gagcgggcag ccccacgttg tcttgcacgg tgaactggcc
     5761 gcctggttcg aagcacgcca gctgaaggcc catgtcagcg tcaccgatga aaccgattac
     5821 gcgaccagct tcgtggtcgt cgagagctgc ccatgaaaac cacacgaacc accgcccgcc
     5881 gtcgcctgtc taagcaagtg cccggcgccg agactccaca tgcaccggtg gtgatcgaca
     5941 tcgacggcct gtgtctggac gccgacgatc ggcgccgcct tcggcatccg ttgactggag
     6001 gattgatcct gttcgcgcgc aactggcagg atcgccatca actgacggag ctcaccgccg
     6061 agatcaagtc gatccggccc gacgtgctca tctgcgtgga tcatgagggc ggtcgcgtcc
     6121 agcgttttcg caccgatggg ttcacccatc tgccgcctat gagggttctc ggcgagcagt
     6181 ggatgaccga tccgatgggt gccaccgatt cggccaccgc gagcggcttc gtgctggcgg
     6241 ccgaactgcg cgcttgcggt gtcgacctca gcttcacgcc ggtactcgat ctcgatttcg
     6301 gatccagcgg tgtgatcggc gaccgtgcat ttcaccgtga tcctcgggtt gtcacgctgc
     6361 tggcgaagag cctgatgcac gggctgctgc gtgcgggcat gtcgcattgc gggaagcatt
     6421 tcccggggca cgggcatgtc aaggccgaca gccacaccga gattcccgtg gacaagcgat
     6481 cgctgaaggc gatcctcgcc gatgacgcgc ggccgtacga gtggttgagc accagcctcg
     6541 ccagcctgat gccggcccat gtcgtctatc cgaaggtcga tgcgaacccc gc
//
LOCUS       DAOORV010000179         6510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14477_length_6510_cov_7.911696, whole genome shotgun sequence.
ACCESSION   DAOORV010000179 DAOORV010000000
VERSION     DAOORV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14477_length_6510_cov_7.911696"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1462
                     /locus_tag="PKV56_16295"
     CDS             233..1462
                     /locus_tag="PKV56_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOX69420.1"
                     /translation="MRLLDRLQTELASKVAATRFGRYDVLQRYRDAFDSSRDRVRRAV
                     EAYSSIVGATCQQAASHHMSRLKSASADEAGTIRFNTVIIDEAARANPLDLFIPMSMA
                     KRRIVLVGDHRQLPHLLDAAIEDDVVRAYGEEDRRQVYQQSLFERLWRQLKARETADG
                     FSRVVMLDTQFRMHPTLGDFVSKNFYESVGLGKIESGRKAEDFVPTVPGFGAVACAWI
                     DVPARLGREEKASSSRQRICEASRIAKEVRTLLSQLPPDMSVGVITFYAAQRDRIFEA
                     LCELGIAETGESGWQIKAEHAGNSTCPERLRIGTVDAFQGKEFDIVLLSAVRSNDLSI
                     NAGADDDPAMYEKAASRRYGHLRVSNRLNVAMSRQRRLLIAVGDHEMFVGIQAQRAVP
                     EMAAFVDLCDSEARHVR"
     gene            1452..2915
                     /locus_tag="PKV56_16300"
     CDS             1452..2915
                     /locus_tag="PKV56_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69421.1"
                     /translation="MFADRFLRWNSGVAGATRGNRRSVLWPVHVWSVMAPDRSPSELN
                     VFQEAILGLLHTGLRDLDELARCLDLDRDLAAFIIATQLQPSGWIDARQQVTPLGVQV
                     LSGLTSDEPSLAVQYAFQDAITGRWLPRLARDLPELEPLPETVGTSPEFVIDRDSGRR
                     IRPFLLAPQTRAGEPDKAGAKQALRHFQRDLRRGRLEEDAYIHDAVSDDFDFVDGAPT
                     AAYVWCDVFVSPSDLQPWLVSDPWRVTEAAAWLREPLLERLGQFPGLAKRIAELIPDG
                     PPSEASVGEWLGQLELRVDLELADLPHLESQPLIREHLARVLRQLRRVEGQNRVHQEE
                     LASLAQESMSAFEAILKWILEKWPVDANGWPEGKWSRKDLASLLQGLPVKAPLSPTVI
                     EALCGQDARQVRLAVLRRDRPLKALLTGALLSTYARPDHPLMDLPARTLQWDRLMAFV
                     AMRNEGSHASGQKLDPSSVLEMARFAVEWHDQFKPHY"
     gene            2928..5321
                     /locus_tag="PKV56_16305"
     CDS             2928..5321
                     /locus_tag="PKV56_16305"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69422.1"
                     /translation="MGKKSKNYGNAPKAQAGANVAAPAPPALITRFLAAVQQAPLASQ
                     DVPDWSETEILGFVEQLAPESRGKLQGFLERFASLGSELKDACGRQAEAQRSAEAAKA
                     KHDEALAALETDRGSLASDRTALEVRAAELAAEAAALASRTGTVLAQEREVMAREAAV
                     RAGLFDEQQRALATLRAQVEALETQRQHLPFAIEEERQALLAAARTLAEGVLAQARDR
                     LAEVEQRQIAIGEQELGLARREERLRLNEVLLKARRESLSNEIRDQFEHELQQKVARI
                     GQLEKQREDLHGRVDRLQTELDEFNDLRDRLGSSPQTLLDELESLKLAKRALDRQVQE
                     LLASRSEDDATLLRGQRDRLQERLQEAENELFSLRHRASEWQRSVTERQDWQLERVAM
                     LKRRELLAEAVQRLQADVDGLVNRQKESTAFPELTRMDVELTVPTVTERAPLLKDLIP
                     DLQARIAFAESGKELHFRREDLQLFLGGLAMSQLHILQGISGTGKTSLATAFAKAVGG
                     VCTTIPVQAGWRDRADLLGHYNAFEKRYYERNTLQAIYRAQTEADKDRLHIVLLDEMN
                     LSRPEQYFAEFLSALELAEGDRWINLMEARPAHGAPSKLRNGRDIWLPPNLWFIGTAN
                     HDETTSAFADKTHDRSFVLDLPKHEPSEGKLKRPTGEVKWRYSSLQEQFDSASIRYQE
                     QIVDLMAFVNASRLTKVLQDDFELGWGNRLERQMGRFVPVVLEAGGTEALAVDHLLCS
                     RMFRDGKVIGRHDVRTDDLKKVEGALFEMWKDCELEGEPSRCLRQLRKDIQRLERGG"
     gene            5318..>6510
                     /locus_tag="PKV56_16310"
     CDS             5318..>6510
                     /locus_tag="PKV56_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69423.1"
                     /translation="MKVFDRYQAIEVDLPKVVEPGRYRLLRRTVLNGSTELPAGDLLW
                     SDGGSLCDLEGEASRTFVGKDDEQEGHIPDSIVDGAVSAVVDQLRTSGADLPSPVMPA
                     RLGDLASATPLELELESVMGRGHLQAIAARPRMAMRYESELLPVSRARRLANDAVTRL
                     ASHSEDWLRREITGVIPRTLKAEVSEDDVAIYENVVFARLLDRLEDVLRRRLREVNVL
                     LRKHDQAMALANAERLDYRLRNALCSLWGLSFVDNPVTGKTATETFLLIEMLLGKVRQ
                     LKRSDLYAAIPRAHRIPLALRSTNVLQHDTHYSCLRPLWLMAHASAQGQAPTPTERYA
                     AEKHQGERFAQYVGLLIRHALASCQLVAAGSLPGQYRVGAKSLTLLWEQDQWALSLEG
                     CATPL"
BASE COUNT         1294 a         1869 c         2097 g         1250 t
ORIGIN      
        1 gtcgtttgcc gacgatggtg cctttcgcgc aggttcactg ctgagtgaag ctggggctca
       61 gccaggcctg ttgacccaag ggcaaactga tctcctgacc ctcgcccaga acgaagaccc
      121 aatcacggac cggcacttgt ccgatctcgc tgaacttcgt gaccaagtcc ttgacctgtt
      181 gcgacccgac aatcggccgg cggcggttcg acatcaactc gacgaagaag gagtgcgcct
      241 tctcgacaga ctgcagacag agctcgcgag caaggtggcc gccacgcgat tcgggcgcta
      301 cgacgttcta caacggtacc gcgatgcctt cgactcgagt cgcgatcgtg ttcggcgtgc
      361 tgttgaagcc tacagcagca tcgtcggggc tacctgccag caggctgcca gtcatcacat
      421 gagccgactc aagtccgctt cggccgacga agccgggacc atacgtttta acacggtgat
      481 catcgacgag gctgcgcgcg ccaacccgct tgacctcttc atcccgatgt ccatggcgaa
      541 gcggcgcatc gttctcgtcg gtgatcatcg gcaactcccc caccttctgg acgcggccat
      601 tgaggacgat gtcgtgcggg cgtatggcga agaggatcgc aggcaggtct accagcagag
      661 cctgttcgag cgcctgtggc gccagttgaa ggcccgcgag acggctgatg gattctcacg
      721 cgtggtcatg ctggacacgc agttccgcat gcatccgacc ctcggggact tcgtaagcaa
      781 gaacttctac gagtccgtgg gcttgggcaa gattgagtcg ggtcgcaagg ccgaggactt
      841 cgtgccgacg gttccggggt tcggtgcggt tgcctgcgca tggattgatg ttcccgcccg
      901 cttgggccga gaggagaagg cgagcagcag ccggcagcgc atctgtgagg cttcgcgcat
      961 cgcaaaggag gttcgaacgc tgctctcgca gttgccgccg gacatgagcg tcggcgtcat
     1021 cacgttctac gctgctcaac gtgatcgaat cttcgaagcg ttgtgcgaac tgggcatcgc
     1081 ggagacgggg gaatcgggtt ggcagatcaa ggcggaacac gctggcaact cgacatgtcc
     1141 ggagcggctt cgcatcggca ccgtcgatgc gttccaaggc aaggagttcg acatcgtcct
     1201 gctgtcggct gtgcgtagca acgacttgag catcaacgca ggcgctgatg acgatcccgc
     1261 gatgtatgag aaggccgcca gccgaaggta cggccacctt cgtgtatcga accgcctgaa
     1321 cgtcgcaatg agcaggcagc gtcgcctgct gatcgctgta ggtgatcacg agatgtttgt
     1381 aggaatccag gcgcagcgtg cggtgccgga gatggccgcc ttcgttgatc tgtgcgacag
     1441 cgaggcccgg catgttcgct gatcgctttc tgcgctggaa ctcgggcgtc gccggcgcca
     1501 cgcgcggcaa tcgaaggtcg gtcctttggc cggttcacgt ctggagtgtg atggcgcctg
     1561 atcgcagtcc cagtgaattg aacgtgtttc aagaggccat tcttggcctg ctacacacgg
     1621 gactgcgtga cctcgatgaa ctggcccgtt gcctggatct ggaccgtgac ttggcggcct
     1681 tcatcatcgc gacccagctt caacccagcg ggtggatcga cgcacgccag caggttaccc
     1741 cgttgggcgt ccaggtgctg tcgggtttga cttcggatga gccaagcttg gctgtgcagt
     1801 atgcgtttca ggatgcgatc actgggcggt ggctcccgag gctagcgcgt gacctgcccg
     1861 agttggagcc gctaccggag actgtcggga catcaccgga gttcgtgatt gaccgggact
     1921 caggccgacg cattcgaccg tttctgctgg cgccacagac gcgtgctgga gaacctgaca
     1981 aggctggtgc gaaacaggcg ctcaggcact ttcaacgtga tctaaggcga gggcggttgg
     2041 aggaggatgc ctacatccac gacgccgtga gcgatgactt tgacttcgtc gatggggcgc
     2101 caacagcagc ctatgtctgg tgcgacgtgt tcgtcagtcc gtcagacctg cagccatggc
     2161 tcgtcagtga cccatggaga gttaccgagg ctgccgcttg gcttcgtgag ccgctgctgg
     2221 agcggctggg acagttccca ggactggcca agcggattgc cgagttgatt cccgatgggc
     2281 cgccctcaga agcgtccgtc ggcgaatggc ttgggcaact tgagctgcgc gttgacctcg
     2341 aactggcgga cttgcctcac cttgagagtc agccgctgat tcgggagcac ctcgccaggg
     2401 tcttgcgaca gcttcgacgc gttgaaggac agaaccgcgt ccatcaggaa gagctggcga
     2461 gcttggctca ggagagcatg tccgccttcg aagccattct gaaatggatc ctcgagaagt
     2521 ggcctgtaga cgcgaatggt tggccagaag gcaagtggag tcgaaaggac ctggccagcc
     2581 tactgcaggg cttgccagtc aaagcgccac ttagcccaac ggtgatcgag gcactgtgtg
     2641 gccaggacgc ccgccaagtg aggttggctg tcctccggag agaccgcccg ctcaaggctc
     2701 tcctgactgg cgcgctgctg tcgacgtatg ccagaccgga tcacccgctg atggatttgc
     2761 cagccagaac cctgcagtgg gatcgcttga tggcgttcgt cgcgatgcgc aacgaaggct
     2821 cgcacgcaag tgggcagaag ctcgatccct caagtgtcct ggagatggcg cggttcgccg
     2881 tcgagtggca tgaccagttc aagccgcact actgaaaaga aagatctatg gggaagaaat
     2941 caaagaacta cggcaatgca cccaaagcgc aggctggcgc caatgtcgcg gcgccggcgc
     3001 cgcccgcatt gatcactcgc ttcctcgctg ctgtccagca agcgcccctg gcttcacaag
     3061 acgttcccga ctggtcagag actgagatct tgggctttgt ggaacagctg gcacccgagt
     3121 cgcgaggcaa gcttcagggc ttcctggagc gttttgcatc gctcggctca gagttgaagg
     3181 atgcgtgtgg ccggcaggcg gaggctcagc ggtccgcgga ggctgccaag gcgaagcacg
     3241 acgaggcgtt ggcggctctg gagactgata ggggttcgct ggccagtgac cgaactgctc
     3301 tcgaagtgcg cgcagccgag cttgcggccg aggcagctgc tctggcatca cgcaccggga
     3361 ccgtgcttgc ccaagagcgc gaggtgatgg ccagagaggc cgccgtccga gccggactct
     3421 tcgatgaaca gcaaagggct ctagccactc tgcgagcgca ggtggaagca ctcgagacac
     3481 agcgtcaaca tcttccgttc gcgattgagg aagagcgcca ggcactgctt gccgcagctc
     3541 gtacgctcgc cgagggtgtg cttgcgcaag caagggatcg acttgccgaa gtggagcagc
     3601 gccagattgc catcggtgaa caggaactcg ggcttgcccg gcgtgaagag cggcttcgcc
     3661 tgaacgaggt gctccttaag gctcggcgcg aatctctttc caacgagatc cgagatcagt
     3721 tcgagcacga acttcagcag aaggtggctc gcattgggca gttggagaag cagcgggagg
     3781 acttgcacgg gcgcgtggac aggctgcaga cggaactcga tgagttcaac gacctgcgtg
     3841 accgtcttgg aagcagcccg caaacgctgc tcgacgaact ggagagcctg aagctggcca
     3901 agagagcgct cgatcgtcag gttcaagaac ttctggccag tcgatcggaa gatgacgcca
     3961 ccttgcttcg cgggcagcga gaccgcctgc aagaacgtct ccaggaggct gagaacgagc
     4021 tcttcagctt gcgtcatcgc gcgtcggagt ggcaacggag cgtgaccgag cggcaggact
     4081 ggcaactcga gcgtgtcgcc atgctcaagc ggcgagaact tctggccgag gctgtgcagc
     4141 gtcttcaagc ggacgtcgac ggcctcgtga atcggcagaa ggaaagcact gccttccctg
     4201 agctcacgcg aatggacgtc gagttgactg tacccacggt cacagagcgg gctccgctgc
     4261 ttaaggacct gatccctgat cttcaggccc gcattgcctt tgctgaaagc gggaaggagc
     4321 ttcatttcag gcgcgaagac ctccaactgt tcctaggtgg tcttgccatg agccaactgc
     4381 acatcctgca ggggatcagt ggcaccggca agacgagctt ggcgactgcc ttcgccaagg
     4441 ctgtgggagg tgtttgcacc acgatcccgg tgcaagccgg ctggcgagat cgcgccgatc
     4501 tcctgggcca ctacaacgcc tttgagaagc gctactacga gcgcaacacg ctgcaggcga
     4561 tatatcgcgc ccagaccgag gccgacaagg acaggctcca catcgtcctg cttgacgaga
     4621 tgaacctgtc tcggcctgag cagtacttcg ccgagttctt gtccgcactc gaattggctg
     4681 aaggcgatcg ttggatcaac cttatggagg cacgaccggc gcatggtgct ccttcaaagc
     4741 ttcgcaacgg gcgggatatc tggctgcctc caaacctctg gttcatcggt acggcaaatc
     4801 acgatgagac aaccagcgcc tttgccgaca aaacccacga ccgttccttt gtgctggacc
     4861 tgccaaagca cgagccgtcg gaaggaaagc tcaagcgacc gacaggtgag gtcaagtggc
     4921 gatacagcag cctgcaggag cagttcgata gtgcgagcat tcgctaccaa gagcagatcg
     4981 tcgatctcat ggcattcgta aacgcgagcc ggttgacgaa ggtcttgcaa gacgactttg
     5041 agcttggatg gggcaatcgc ctagagcggc agatgggacg gttcgtcccg gtcgtgctgg
     5101 aagctggcgg taccgaagca ttggcggtcg atcatctgct ttgcagcagg atgttcaggg
     5161 atggcaaggt cattggtcgg cacgatgttc gtactgatga cttgaaaaag gttgaaggcg
     5221 ccctgttcga gatgtggaag gactgcgaac tggagggtga gccgtctcgt tgcctgagac
     5281 aactgcgcaa ggacatccaa cggttggagc gcggcgggtg aaggtgtttg atcggtacca
     5341 ggcgattgag gtcgacctac cgaaggtagt cgagcccggt cgctaccgcc tgttgcggcg
     5401 caccgtattg aatggttcga ccgagctacc agccggagat cttctctggt ccgatggtgg
     5461 gagtctgtgc gatctcgaag gcgaagcttc gcgcacgttc gttgggaagg atgacgaaca
     5521 ggaaggccac attccagatt ccattgttga cggtgcggtg agcgctgtgg ttgatcagct
     5581 aaggactagc ggcgctgatc tgccctcgcc ggtaatgccg gcgagattgg gtgatctcgc
     5641 ctcagccacg ccacttgagc tggagctcga aagcgtcatg ggccggggac accttcaggc
     5701 catagcggct cgtcctcgga tggctatgcg ctacgagagt gaactgctgc cagtgtcccg
     5761 agcgaggcgg ctggccaacg acgcagtcac tcggctcgct tcccactcag aggattggtt
     5821 gcgccgagag ataaccggcg tcatccctcg gaccctcaag gccgaagtca gcgaggacga
     5881 cgtggcgatc tacgagaacg tcgtcttcgc acgcttgctt gatcgtctgg aagatgttct
     5941 tcggcggcga cttcgcgaag tgaacgtgct actaagaaaa cacgatcagg cgatggcgct
     6001 cgccaatgct gagcgacttg actaccggct gcgaaacgca ttgtgcagcc tctggggcct
     6061 gtcattcgta gacaacccgg tcaccgggaa gacggctacc gagacgttcc tgttgatcga
     6121 gatgcttctc ggcaaggtac gacaactcaa acgtagcgat ctttacgcag ccatcccgcg
     6181 cgcccatcgg attccattgg cgttgcggag caccaatgtg ctccagcatg acacccacta
     6241 cagttgcctt cggccacttt ggctcatggc acatgcctcc gcgcaagggc aggccccgac
     6301 cccgacggag aggtatgcag cggaaaagca tcagggcgag cgattcgcgc agtatgttgg
     6361 tttgctgatt cgtcatgccc ttgcgtcgtg ccagctcgtg gcggcgggca gcctgccggg
     6421 ccaatacagg gttggcgcaa agtcgctcac cttactttgg gagcaggatc agtgggcgct
     6481 ctcgcttgag ggctgtgcga cgccgctgca
//
LOCUS       DAOORV010000180         6496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14519_length_6496_cov_9.156963, whole genome shotgun sequence.
ACCESSION   DAOORV010000180 DAOORV010000000
VERSION     DAOORV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14519_length_6496_cov_9.156963"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PKV56_16315"
     CDS             <1..1636
                     /locus_tag="PKV56_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HOX69424.1"
                     /translation="QGECVAANQSSVQAIEALKAQDRGEQQRLDRLQQRVAHRTSQER
                     SRWSALQDRRCKYLDALKQAAAVRRAERRLPLAEIVLAARVARVTTWRRQVQCLTECQ
                     GGIRTVEQKLSATLREAGQAAIRADDLARRFGLTNAVPCAGTDLQGQCQLLGDAREAS
                     TLIPSAQAAMARLAREKASIDAELATLRSQREALSGAPMTLSRVEVKCERARARATRL
                     AQWSAKSTEIAQARAALTEIDQELAVAASPGAADGQPVETTEEHAERHQIGATRQQIA
                     NQIEQQSHQLRATLDRLHAALGTMPAAFDVRRLTEAKSAMDLAARAAVAAEATHLDAV
                     RDAEALAGLTLQAAALATRQSLAASRIARVESELSNWNLFARCMSNDGLIALAIDDAG
                     PALSGLANDLLLACYGTRFTVSILTLLETGKGDQKEGFDIVVHDGESGESKSVGLMSG
                     GERVFVNECLIRAVALYLAQHTGRQYDTLFSDEADGPLDPERKRMFMAMKREVLKLGG
                     YAREFFVSQTPELTAMADAVIDLEVFAAARDSVVAA"
     gene            complement(1787..2095)
                     /locus_tag="PKV56_16320"
     CDS             complement(1787..2095)
                     /locus_tag="PKV56_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69425.1"
                     /translation="METPSIAGLRQQAELLAALSINHLTSATRRRLADDDLSVNAYPT
                     DAGGFVYVGTPRYRIPTEPDLAAIFEAAEQAGVAWLMFDREAAVVDGLPVFEVAQPGP
                     "
     gene            complement(2379..2954)
                     /gene="radC"
                     /locus_tag="PKV56_16325"
     CDS             complement(2379..2954)
                     /gene="radC"
                     /locus_tag="PKV56_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX69426.1"
                     /translation="MNVTALSREDLVSEMLAPLPPTFAAGALRVSDADLSAPPSPESL
                     LARRLSVARELLLRDLREQMHRGPVMTSPQILRDWLRLYCAGLEHEVFLVLYLDANHR
                     LIEPQELFRGTLTQTSVYPRELVKGALARNAAALAVAHNHPSGQAEPSRADEFLTQTL
                     KSTLSLVDVRIIDHFVVAGDQVVSFAERGLI"
     gene            3428..3643
                     /locus_tag="PKV56_16330"
     CDS             3428..3643
                     /locus_tag="PKV56_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69427.1"
                     /translation="MIRLGSQIRLSRREVERFLKITDIEPVGVRTIDDLEAYIARCKA
                     HYWGVSKETRFLHWLIDREAAQCRQAA"
     gene            complement(3697..4200)
                     /locus_tag="PKV56_16335"
     CDS             complement(3697..4200)
                     /locus_tag="PKV56_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69428.1"
                     /translation="MSPEKPRPRARRCGIEFEEERAWVGFYKRVGRDPAIAAEVMAQL
                     EADVDMKRTHLALYLCCKESLRVHKARQARNKRIGQFVRGLGHALLVQPMLGLRTRLR
                     HGGDIALECLPDTRKEPALQQVRRLGRDAEYAAAQASFEQQVSSTVAVKTTADASAVP
                     KASAAAA"
     gene            complement(4550..4771)
                     /locus_tag="PKV56_16340"
     CDS             complement(4550..4771)
                     /locus_tag="PKV56_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69429.1"
                     /translation="MLTMVVSLYRRRVAATVIELAKDDPSLVKEVIARLRAAGEIELD
                     DLVYLDRIADRWISIAEGNRHKARSAHPR"
     gene            complement(5573..6349)
                     /locus_tag="PKV56_16345"
     CDS             complement(5573..6349)
                     /locus_tag="PKV56_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69430.1"
                     /translation="MTFIHRDPLGNIPVESREGASEALAQALNIRNRESKRRRFGFST
                     SEDAVTWVVFMHLLRSGQLLGSLRQAGLITDSALMATPTLLLWGAPVDAGARGKEIQG
                     RLRELCAGLREDPNSFSEPDVIVDFGEHGVMFIEVKHQSGNDLKPVDYAGWPRYASAA
                     PLAWRIEDVKGSGCYELARNWCLVRLLSDGRPATLVNLGPSRLFGGAEGARLDRFVTA
                     LDTDDRSRFAKVAWSDLLTQGLADAPGWFSRFCRERGLIV"
BASE COUNT         1168 a         2059 c         2258 g         1011 t
ORIGIN      
        1 gcagggcgag tgtgttgccg ccaaccagtc cagcgtccag gccatcgagg cgctgaaggc
       61 gcaggaccgg ggcgagcagc agcggctgga tcgcctgcag cagcgcgtcg cgcaccgcac
      121 ttcgcaggaa cgcagccgct ggagcgcgct gcaggatcgc cgatgcaagt atctcgacgc
      181 cctgaagcag gcggcggccg tgcggcgtgc cgagaggcgc ctgccgctgg ccgagatcgt
      241 cctcgcggca cgcgtggcaa gggtgactac ctggcgccgg caggttcagt gcctgaccga
      301 gtgccagggc ggcattcgca cggtcgaaca gaagctctct gcaaccctgc gtgaggcggg
      361 acaggcagcg atcagggcgg acgacctggc gcgtcgcttc ggtctgacga atgccgtgcc
      421 ctgcgccggg accgacctgc aggggcaatg ccaactgctg ggtgatgcgc gggaggccag
      481 taccttgatc ccgagcgccc aggccgcgat ggcccgcctg gcgcgcgaga aggcatcgat
      541 cgacgcggaa ctggcgacgc tgcggtcgca acgggaggcg ctgtccggcg cgccaatgac
      601 cctgtcacgg gtcgaagtca aatgtgaacg cgctcgcgca cgggccacac ggctcgcgca
      661 atggtccgcc aagtccaccg agattgcaca ggcgcgtgca gccctgaccg agatcgacca
      721 ggaactggct gtggctgcat cgcccggtgc agccgatggg caacccgtgg agacgaccga
      781 ggaacacgcg gagcgccacc agatcggcgc cacgcggcag cagatcgcca accagatcga
      841 gcagcagtcg caccaacttc gcgccacgct ggaccgactg catgcggcgc tgggcaccat
      901 gcccgctgcc tttgacgtgc ggcgactgac cgaagcgaag tcggccatgg acctggcggc
      961 gcgagcagcg gttgcggccg aagcgactca cctggacgcg gtgcgggatg ccgaggcgct
     1021 ggcgggcttg acgctgcagg cggcggctct ggcgacccgg caatccctgg cggcgagccg
     1081 catcgcccgt gtcgaatccg aattgagcaa ctggaacctg ttcgcccgct gcatgagcaa
     1141 cgacggactg atcgcgctgg ccatcgacga cgcgggcccg gccttgtcgg ggttggccaa
     1201 cgacctgctg ctggcctgct acggcacccg cttcacggtc tccatcctga ccttgctgga
     1261 gaccggcaag ggcgaccaga aggagggctt cgacatcgtc gtgcatgacg gcgagtccgg
     1321 cgagagcaag agcgtcggcc tgatgagcgg cggcgagagg gtcttcgtca acgagtgcct
     1381 gatccgggcg gtggcgctgt acctcgcgca gcacaccggg cggcagtacg acacgctgtt
     1441 ttccgacgag gccgatggac cgctcgatcc cgagcgcaag cgcatgttca tggcgatgaa
     1501 gcgggaggtg ctgaagctcg gtggttatgc ccgcgagttc ttcgtgtcgc agacgccgga
     1561 gttgacggcc atggccgacg ccgtgatcga cctggaggta tttgctgctg cgcgcgacag
     1621 cgtcgtggcc gcgtgagttg cggccacaaa aagtcgcggc gagggaggaa cggagcaggg
     1681 cgtcggcctt gtcagcgcga cgccccgcgg tgcaggatca ctgttccggg aacggctgat
     1741 caatgggtcg gcaagttctc cggcggctgg tcggcgccgc ggtgactcat ggcccaggct
     1801 gcgcgacttc gaacacgggc aggccatcga ccacggccgc ctcgcggtcg aacatcagcc
     1861 aggcgacgcc ggcctgctcc gcggcctcga agattgcggc cagatccggc tccgtaggaa
     1921 tgcggtacct cggggtgccg acgtagacga agccgccggc atcggtcgga taggcattga
     1981 ccgacagatc gtcatcggcc agtctccttc gggtggccga cgtcagatgg ttgatcgaca
     2041 gggcggccaa caactcggcc tgctggcgca ggcccgcgat ggatggtgtt tccatggtgg
     2101 actcctccag gagtggaagg tgactcggac gcttggcgcg cacgcatgcg ttgacggttc
     2161 atgcggccaa acgtcatgcc gcagtcgcgg caacggaacc agtgcaggtg acccagcgcg
     2221 cccagcggca cgccgtggcc tgcacagagg gggcagggcg gtggggttct cgccgacatg
     2281 gcggccattt ctgtggatgg aaggggccag ccgccccgac ggggctgact ggcccctcgg
     2341 ggttgaacac gcacggaggt tggacctcgt gcatgacgtc agatcagtcc ccgttcggcg
     2401 aagctgacga cctggtcacc ggccacgacg aagtggtcga tgatgcgcac gtccaccagg
     2461 ctcagcgtgg acttcagcgt ctgagtcagg aactcatccg cacggctggg ttcggcctgg
     2521 ccgctggggt ggttgtgggc caccgccagt gctgccgcgt tgcgggccag cgcacctttc
     2581 accagttccc gggggtacac cgaggtctgg gtcagcgtgc cgcggaacag ttcctggggc
     2641 tcgatcagac ggtggttcgc gtccaggtac agcacgagga agacctcgtg ttccaggcct
     2701 gcgcagtaca gccgaagcca gtcgcgcaag atctgcgggg aagtcatcac ggggcccctg
     2761 tgcatctgct cgcgaaggtc gcgcagcagc aactcgcggg ccacgctgag ccgacgggcc
     2821 agcagggact ccggtgacgg gggtgccgac aggtcggcat cgctgacacg cagcgctccg
     2881 gccgcgaagg tcggtggtag cggggccagc atctcgctga ccaggtcctc acgggacagg
     2941 gcagtcacat tcatggcgtt ctcctctcta agggaaagaa ggcgggaacg ccgctcccgc
     3001 tggggacgag gtccccgcgc gggaagaaag tgcagacggg tgtgtcggca cggttgcaaa
     3061 ggcaggtatc gactgcgacg ccggcatgtt cgccggacag cactccaaga gtgagtacaa
     3121 caaagcctga gaagtctgat ccaacaggcg gcaagattca agcgggcgag cagggagcct
     3181 cgctcaaccg gtggcaatct ggccggcttc gaggtggtgc ccagctgtcg ggaaactggc
     3241 gcggcggttc tgttgccaat ctccgacgtc gtcgtcggga tttgacgcgt ctggccggcg
     3301 ccaatggcac tgcagcgtca ctccaggatc actgcagaaa tttcgttcgc acctcgcccg
     3361 acaggtgcgc cagctcgccg atttccagcc aagatgatcg gcagtgcact caaccgaccg
     3421 accgaccatg atccgcctgg gctcacagat ccgcctctcc cgacgggagg tcgagcgctt
     3481 cttgaagatc accgacatcg agccggtggg cgtccgcacg attgacgacc tggaggccta
     3541 catcgcgcgc tgcaaggcgc actactgggg cgtgtcgaag gagacccgct tcctgcactg
     3601 gctcatcgac cgagaagccg cccagtgtcg tcaggcggcg tagtaccggt gctgcttcga
     3661 acaacgtgcc cgcgggcacg ttgttcggga gcaatctcaa gccgccgcgg ctgacgcttt
     3721 cgggactgct gacgcgtcgg ccgtcgtctt gaccgccacc gtcgaactga cttgctgttc
     3781 gaacgaggct tgcgccgcgg cgtactcggc gtcccgaccc agtcggcgga cctgctgaag
     3841 cgcaggctcc ttccttgtgt cgggcaggca ctccagggcg atgtcgccgc catggcgcaa
     3901 ccgcgtacgc agcccgagca tcggctgcac gagaagtgca tggcccagtc cacgaacgaa
     3961 ctggccgatc cgcttgttgc gcgcctgccg ggccttgtgc acccgcaagg attccttgca
     4021 gcagaggtac agcgccaggt gggtgcgttt catgtcgacg tcggcctcga gttgggccat
     4081 gacctcggcg gcgatggccg ggtcgcggcc gacgcgcttg tagaagccga cccaggcgcg
     4141 ctcttcttcg aactcgatgc cgcagcgcct tgcgcgaggg cggggtttct caggggacat
     4201 ggtggttctc cagtggtgag caaagggaaa ccctgtcccc gaacggggag acggtggact
     4261 ccccgggggt tgcttggatg acgggccgaa ggtctggcgc taagctgcgc cggcgccctt
     4321 gacgagtgcc cacacgatgt ttcgcgcagg tcacacccta cggttcacgc ccgacgaaat
     4381 cgaggggttc cgaaaactcg gactcgactt cgacggggcg cgaacacagg acgatgtcga
     4441 acaggtgctg acccgctggg ctgacacact gaacgacgaa cgcccagatc tgctggacag
     4501 gattgcagcg gaactggcca agaccaaagg tgtccacttg cccgcgcgac taacgcgggt
     4561 gcgctgacct ggccttgtgc ctgttgcctt cggcaatgct gatccagcgg tcggcgatcc
     4621 ggtccagata gaccaggtcg tcgagctcga tctcgcccgc cgctcgaaga cgggcaatca
     4681 cttccttgac cagggacggg tcgtccttgg ccagctcgat cacggtggcc gcaacgcgtc
     4741 tgcggtagag gctgaccacc atggtcagca tcccgcgatg cgttgctcac gtgccatgcc
     4801 ggaccgcccg agggcgcagg gcaatggctt caacagcgcc ggaacgacgg gtgcgcgcag
     4861 agtggacatc cgggcaagcc cggagccacc cagcaaaaca ccgacggggc gcgagtctcg
     4921 gttcgcaggg actgcgagca tggggttctc cttcggttgg aagaggaacg cccatccctg
     4981 gcggggaaca tggggttccc cgtttgggat gctccaccgc gaacggcaaa gcggctcggt
     5041 ggcgcgccgg gtggcacgca caggaagaag gcccagggtc cttgcggaca gggccttcgc
     5101 tcgatgccga tgtcagccag cgcgcgaagc gatggcatcc acggcatgca ggtcgccact
     5161 cgacccgagg gtcgcgcggc ggggcacaca gatcagcctg acggctgaac ggaacactcg
     5221 gccatgggca tggccggacc cgaccgaggc cggggatcca caaggcaggt aacgactgcg
     5281 acgccagcga agctggactt cagggcgagc aggcccgccg atgaaggctg tgcagacgcg
     5341 atgtccgcaa agagcactgc aagtgttgag cgaacaccgt gatgcttcaa cgagccgaag
     5401 caaagcgcgt tgcgaagctc ggcgcaaggc ccgtcaattc tggtggcttg gcggtggcga
     5461 acggatggcg gccgcgtaga gccgttccgt cgggcgagtg catgggttcc ttgcgcgtgt
     5521 acgactggcg aaggcgtgct ccgccgctac agcggcaccg gctcgcagca gctcaaacga
     5581 tgaggcctcg ctcccgacag aaccgcgaaa accagccggg cgcgtcggcg agcccctgcg
     5641 tcaggagatc ggaccacgcg acctttgcga aacgtgacct gtcgtcggtg tccagcgccg
     5701 tcacgaaacg atccaggcgt gcgccttcgg caccgccgaa cagtcgggac ggacccaggt
     5761 tgaccagggt cgcaggacgg ccgtcggaaa gaagtctgac gagacaccag ttccgggcca
     5821 gttcatagca gcccgagcct ttgacgtcct cgatccgcca ggcaagtggc gccgccgagg
     5881 catacctggg ccaacctgcg tagtcgacag gcttgaggtc gttgccgctc tggtgcttga
     5941 cttcgatgaa catgacgccg tgctcgccga aatcgacgat cacgtcaggc tcggaaaacg
     6001 aatttgggtc ttctcggagg ccggcgcaca gctcgcgcaa acgcccttgg atctcctttc
     6061 cgcgggcacc ggcgtcgaca ggtgcgcccc acaagaggag ggtcggtgtt gccatcaagg
     6121 cgctgtcggt aatcaatccg gcctgccgca gggagcccag caattgaccg gagcgcagaa
     6181 ggtgcatgaa gaccacccag gtcacggcgt cttccgaggt ggaaaagccg aagcgtcgcc
     6241 gcttggattc gcggttgcga atgttgagcg cctgggccag tgcctcgctc gcgccctcgc
     6301 ggctctcgac cgggatgttc ccgagcgggt cgcggtgtat gaaagtcacc gcgcccctgt
     6361 cggaggcgtt cgatgcacca atgccgcacg gctcgcagcg gcgtaggcaa tcatcgaact
     6421 gggtcgccac gggaaccttg cctgcggatt cgacgcctgc gatcggcaag tggcaactcg
     6481 gacagatcag cgatgg
//
LOCUS       DAOORV010000181         6468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14593_length_6468_cov_6.303446, whole genome shotgun sequence.
ACCESSION   DAOORV010000181 DAOORV010000000
VERSION     DAOORV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14593_length_6468_cov_6.303446"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="pilQ"
                     /locus_tag="PKV56_16350"
     CDS             <1..974
                     /gene="pilQ"
                     /locus_tag="PKV56_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOX69431.1"
                     /translation="NSGQGGGGGGGGGGSTNTKMLSPRGSVLYETRTNQLFVSDIPSK
                     LEEIQSLIAKIDIPVRQVLIEARIVEADDSFGRSLGVRLGFNDARGFGVGNGQRVGVG
                     GNLNAVAFQTGQLDAKTIPITDTNFVSLPATAQGGYNPATFALSLFGSSLNRFLNLEI
                     SALEADGRGKVVSSPRIVTADQIKAIIQQGTKIPYQRATSSGATSIEFVDATLKLEVT
                     PQITPEGNVILNVEVNKDSVGAATQGVFSIDTKSIKTQVLVENGGTVVIGGIFEQTDR
                     SDVVKVPLLGDIPGLGNLFKTRTNSSRKTELLIFLTPKVVSERTAAR"
     gene            1009..1575
                     /locus_tag="PKV56_16355"
     CDS             1009..1575
                     /locus_tag="PKV56_16355"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOX69432.1"
                     /translation="MFVSLIGMPGGGKSTVGRHLARRLGVPFSDSDAVIERQIGCSIR
                     EFFDAEGEERFRDVEQEVIAELVRADRGVLATGGGVVLREANRHALRRHTTAVYLRST
                     PEELFRRLRHDTQRPLLQVQDPLARLRTLFQVRDPLYQSVAHFVIETGRPSVQNLVNM
                     LLMQLELAGVLPMDSVPSPVDGIEPSVH"
     gene            1616..2725
                     /gene="aroB"
                     /locus_tag="PKV56_16360"
     CDS             1616..2725
                     /gene="aroB"
                     /locus_tag="PKV56_16360"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOX69433.1"
                     /translation="MPTNPISMTPGAKVSIDLADRRYDILIGSGLLERSGDLACGPQA
                     SEALIVTNTTVAPLYAERVQRALALHHARVHTIALPDGEVHKDWDTLNLIFDKLLKEG
                     FDRKVVLYALGGGVIGDMTGFAAACYMRGVSYVQIPTTLLAQVDSSVGGKTAINHPAG
                     KNMIGAFYQPLKVIADLDTLDTLPDREISAGLAEVIKYGPIADDDFMGWIEKNIDALR
                     ARDKAALAHAIQRSCEIKAWVVGQDERESGLRAILNFGHTFGHAIEAGLGYGEWLHGE
                     AVGCGMVMAADLSARCGLIDAAYAARIAALIERAGLPIRGPRLGAARYLELMRIDKKS
                     ESGQIRFVLIDSPGRASMHRADDALVAQVIADHTA"
     gene            2733..3917
                     /locus_tag="PKV56_16365"
     CDS             2733..3917
                     /locus_tag="PKV56_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOX69434.1"
                     /translation="MNSNHGGELAAYASHPQQSRGRRHAEPASPGRNEFQRDRDRIVH
                     STAFRRLVYKTQVFLNHEGDLFRTRMTHSLEVAQIARSIARPLGLHEDLVEAVALAHD
                     VGHTPFGHAGQDALNECLHRIQPQREGFEHNFQSLRVIDLLEARYPRFDGLNLSFETR
                     EGVLKHCSQTNARRLESVEPGGIGQRFLMRTQPSLEAQLCNLADEIAYNAHDIDDGVR
                     SGLLTIEQLEAAAVFDRHRIESLRDHPALSGRRLLFEAIRRMLSAQIHDVISSTRAML
                     RQHDPQSADQVRGLPPLVCFSPSMATDSAQLKHFLSRHLYRHPQVMDTTDRARSVVRG
                     LFDRYFEDPKSLPESATSRSNAAQDLVRQRPREVADYIAGMTDRFALREHERLTGQRL
                     FD"
     gene            complement(3945..4559)
                     /locus_tag="PKV56_16370"
     CDS             complement(3945..4559)
                     /locus_tag="PKV56_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69435.1"
                     /translation="MKPAGHWHGLAAALLGAATLHVAAAVLMSRAAPGATSRAAAGVD
                     QRRLTIELRPAALTAPSFEETAELPPEPSGEEITAAPVAEADPQSTSIGAPLPLADPE
                     ADESARYFNVGEVDTPAVPRPDWEVDVALLMGMGVRSFSIDVLINEMGTAEQCAVTRI
                     EPDQSAELRHAVAAKFCETVLSPAKRRGVAVRSIRHIELLLAPP"
     gene            complement(4603..5118)
                     /locus_tag="PKV56_16375"
     CDS             complement(4603..5118)
                     /locus_tag="PKV56_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HOX69436.1"
                     /translation="MSKHCMSNPGPVPRCHQRGRLPASGFTLIELMITVAIVAILAAV
                     AYPSYREYIDRSRRSEAQSLLMEAAQWMERFYAENYRYDQNTAGVAVADLFGARFSQS
                     PRSGAASYTIGVPTATQTEFVVRATRTGSMTGNKCGNFEITGTGVRQLETGTFDASLG
                     DQAQAMSRCWR"
     gene            complement(5121..>6468)
                     /locus_tag="PKV56_16380"
     CDS             complement(5121..>6468)
                     /locus_tag="PKV56_16380"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOX69437.1"
                     /translation="FSPLGRGGKGIFALDVTEAGTITIDTKGTETTADDTTSVVGSQL
                     DQAHATTVFAWQMTDADDPEGDMGYMIAEPTTSIFTAQPGQIARMNNGRFAALFGNGI
                     DSTSGRAALYIVFADGDAAAAGGKFKKIVVPTNGANGLSQPTWVDVNNDRIPDYIYAG
                     DIQGNLWRFDVRSADPGAWRVSYFEKPLFTAKDASGTALPITGAPEFSFHRLGGIVVN
                     VVTGKSVTSDDFPNGSRTNGMFGIWDKPAFAVIDEATDMEDALPRDLSDLEERELHNV
                     GTDTQNRYLTGDAIDWNTKLGWYFFFNVNSEMGLNNLTIANNQVLTVTVSPAPPKTGA
                     ATDACSDTPVARLLGVDPITGLPNGLLGTTDVEEGDVTITYQLASIAAVDQKARIGNT
                     QVGEGGNGGDPDKADPCRGGSLDCTKVISARGEINLSSNLGKGRIFWREIPGFKTN"
BASE COUNT         1151 a         2034 c         2073 g         1210 t
ORIGIN      
        1 agaattctgg ccagggtggt ggcggtggtg gtggcggcgg cggctccaca aacacgaaga
       61 tgctctctcc ccgaggcagc gtgttgtacg agacccgcac caaccagctt ttcgtcagcg
      121 acattccgtc gaagctggaa gaaattcaaa gcctcatcgc gaagatcgat atccccgtca
      181 ggcaggtgct gatcgaggcc aggatcgtcg aagccgacga ctcgttcggt cgctcgctcg
      241 gcgtgaggct cggattcaac gacgcacgcg gattcggcgt cggtaacggt cagcgcgtcg
      301 gtgttggtgg caatctgaat gcggtcgcct tccagacggg tcagttggac gccaagacca
      361 taccgatcac cgacaccaat ttcgtgagcc ttccggccac ggctcagggt ggctacaacc
      421 cggccacctt tgcactgtcg ctctttgggt caagtctcaa ccggttcctg aacctggaga
      481 tctccgcgct ggaagccgat ggccgaggga aggtcgtttc cagtccgcgc atcgtgaccg
      541 cggaccagat caaggcgatc atccagcaag gtaccaagat tccgtatcag cgcgccacgt
      601 cgagcggtgc cacttcgatc gagttcgtcg acgcgacatt gaagcttgaa gtgacgccgc
      661 agatcacccc cgaaggcaac gtgatcctga atgtcgaggt gaacaaggac agcgtgggtg
      721 cggccaccca gggcgtgttc tcgatcgata ccaagtcgat caagacgcaa gtgctagtcg
      781 agaacggggg cacggtcgtg attggcggga tcttcgagca aaccgatcgg tcggacgtcg
      841 tgaaggtgcc cttgctgggt gacatcccag ggctcggcaa cctgttcaag acgagaacca
      901 attcgtcgcg caagaccgaa ctgctgatat tcctgacccc caaggtcgtc agcgagcgca
      961 cggcagcgcg ttgattccag gcccgtcggt gccgggcttc gagcgagcat gttcgtcagc
     1021 ctgattggca tgcccggcgg gggcaagtcg actgtcggca ggcatctcgc ccgccgtctc
     1081 ggcgtgccgt tcagcgacag tgatgcggtg atcgagcgcc agatcggttg cagcatccgg
     1141 gagttcttcg atgccgaggg cgaggagcgc ttccgagacg tcgagcagga agtgattgcc
     1201 gaactggtgc gcgccgatcg aggcgtgctt gccaccggag gcggcgtggt cttgcgtgag
     1261 gccaaccgtc acgcgttgcg ccggcacacc acggcggtct acctcagatc gacacccgag
     1321 gaactcttcc gcaggttgcg tcatgacacc cagaggccgc tgctgcaggt gcaggaccct
     1381 ctggcccggc tgcgcacgct gttccaggtt cgcgaccccc tctaccagag cgtcgcgcat
     1441 ttcgtcatcg agaccggacg gccatcggtg cagaacttgg tgaacatgct gctgatgcaa
     1501 ctggaactcg cgggtgtgct gccgatggac agcgtgccct cgccggttga tggaatcgag
     1561 ccgtcagtcc actgatccac cacggctgcg tccggcagcg cggcaaaata gccggatgcc
     1621 gacaaacccc atatccatga cgcccggcgc gaaggtcagc atcgatctgg ccgatcgacg
     1681 ttacgacatc ctgatcggat ccggactgct tgagcgatcc ggagaccttg cctgtggtcc
     1741 acaggcgtcc gaggccttga tcgtcaccaa cacgacggtg gcgccgctgt acgccgagcg
     1801 tgtgcaacgc gcgctggcgt tgcaccacgc tcgagttcac acgatcgcct taccggatgg
     1861 cgaagtacac aaggactggg acacgctgaa cctcatcttc gacaagctgc tgaaggaagg
     1921 cttcgaccga aaggtcgtcc tttatgcgct tggtggtgga gtgatcggcg acatgaccgg
     1981 ttttgctgct gcctgctaca tgcgaggcgt ctcgtacgtg cagataccga cgacgctgct
     2041 ggcgcaggtc gattcctccg tcggaggaaa gaccgccatc aatcacccag caggcaagaa
     2101 catgatcggc gccttctacc agccgttgaa agtcattgcc gatctcgaca cgctcgatac
     2161 cttgcccgat cgagagatct ctgcgggcct ggccgaagtg atcaagtacg gcccgatcgc
     2221 agatgacgac ttcatgggct ggatcgagaa gaacatcgac gcgctgcgcg cccgggacaa
     2281 ggccgcgctg gcgcatgcga tccagcgttc ctgcgaaatc aaggcctggg tcgtcggcca
     2341 ggacgagcgc gagtcgggct tgcgcgcgat cctcaatttc ggacacacct tcgggcatgc
     2401 gatcgaggcc ggactcggct atggcgagtg gctgcatggc gaggcggtgg gttgcggcat
     2461 ggtcatggct gccgacctgt ccgcgcgctg cggcttgatc gatgcggcat atgccgcacg
     2521 catcgcagcc ctgattgagc gtgccggctt gccgattcgc gggcccagac tcggcgctgc
     2581 gcgatatctc gagctgatgc gaatcgacaa gaagtcggag tctggtcaga tccggttcgt
     2641 gctgatcgac tcgcccggcc gggccagcat gcaccgagcc gacgatgccc tggtcgccca
     2701 agtgatcgcc gatcacaccg catgacatcc gcgtgaacag caaccacggc ggtgagctcg
     2761 ctgcctatgc gagtcatccg caacaaagtc gaggccggcg ccacgccgaa cccgcttcgc
     2821 ccggacgcaa cgagtttcag cgtgaccgcg atcgcatcgt gcacagcacc gcgtttcgcc
     2881 ggttggttta caagacccag gtgttcctga accacgaagg ggatctgttc cgaacccgca
     2941 tgacccattc gctggaagtg gctcagatcg cccgatcgat cgcccgcccg ctgggcctgc
     3001 acgaggatct ggttgaagcc gttgcactgg cgcacgatgt cggacacacg cctttcggac
     3061 atgccgggca ggatgcgctg aacgagtgcc tccatcgcat tcagccgcag cgcgagggct
     3121 tcgaacacaa cttccagagc ctgagggtga tcgacctgct ggaagcgcgc tacccccggt
     3181 tcgacgggct gaacctgagt ttcgagaccc gcgagggcgt gctcaagcac tgttctcaaa
     3241 ccaacgcgcg ccggctcgaa tcggtcgagc cgggcggcat cgggcagcgc ttcctgatgc
     3301 gcacgcaacc cagcctcgaa gcccagttgt gcaatctcgc tgacgagatc gcctacaacg
     3361 cgcatgacat cgatgacggc gtgcgctccg ggttgctgac catcgagcaa ctcgaagccg
     3421 cggccgtctt cgatcgccac cggatcgaat ccctgcgaga ccatccggct ttgtctgggc
     3481 ggcgattgct gttcgaagcc atccggcgca tgctttcggc tcagattcac gacgtcatca
     3541 gcagcacccg ggccatgctg cggcagcacg atccgcagtc agccgatcag gtgcgaggcc
     3601 tgccgccgct ggtttgcttc agcccttcga tggccaccga cagtgcacag ctcaagcatt
     3661 tcttgagccg ccatctctac cgacatccgc aggtgatgga caccaccgat cgcgccagat
     3721 cggtggtgcg aggtctgttc gatcgctatt tcgaggatcc gaagtcgttg cccgaatcgg
     3781 caacaagccg gtcgaatgca gcgcaggatc tggtgaggca acgcccgcgc gaagtggctg
     3841 actacatcgc cggcatgact gatcgctttg ccttgcgcga acacgagcgg ttgacgggac
     3901 agcgtctgtt cgactgagtc ctggagcctc gcgcgcgcag caactcaggg cggggcgagc
     3961 agcagttcga tgtgccggat gctgcgaacg gcgaccccac ggcgcttggc ggggctcagc
     4021 acagtttcac agaacttcgc ggccaccgca tggcgcagct cggccgattg atcgggttcg
     4081 atccgcgtga cggcgcattg ttcggcggtg cccatctcgt tgatgagcac atcgatgctg
     4141 aagctgcgca cgcccatccc catcaacagc gcgacatcga cttcccagtc cggccgaggc
     4201 actgccggcg tatcgacctc gccgacgttg aaatagcgcg cgctttcgtc cgcctcggga
     4261 tctgccagtg gcagcggtgc gccgatggag gtcgattgcg gatcggcttc tgcaacgggc
     4321 gcggccgtga tttcctcgcc cgagggttcg ggcggaagct cggcagtttc ctcgaagctg
     4381 ggtgcggtca gagccgccgg acgaagctcg atcgtcaggc gccgctgatc aacgccagcg
     4441 gcggcgcggg aagttgcacc gggggctgcg cgcgacatca atacggcggc ggccacatgc
     4501 aacgtggctg cgcccaggag tgccgcggcg agcccgtgcc agtgccccgc tggtttcatg
     4561 gcgaatggcg atggtctgtc tggcctgtcc caggtcagcg cgctaacgcc agcagcgcga
     4621 catggcttgc gcctggtcgc ccagggacgc gtcgaaggtg ccggtttcca gttggcgcac
     4681 acccgtgccg gtgatctcga agtttccgca cttgttgccg gtcatcgatc cggtgcgggt
     4741 ggctcgaacc acgaactcgg tctgcgtggc ggtcggaacg ccgatcgtgt acgacgccgc
     4801 gccagagcgg ggcgactggc tgaaacgtgc accgaacagg tccgccacgg ccacaccggc
     4861 tgtgttctgg tcgtaccggt agttttctgc gtagaaccgt tccatccact gagcggcttc
     4921 catcagcagg ctttgcgctt ccgaacgccg gctgcgatcg atgtactcgc ggtacgacgg
     4981 gtaagccacg gcagcgagga tggcaacgat ggcgaccgtg atcatcagct cgatcagcgt
     5041 gaatccggag gcaggcagcc gtcccctctg gtggcaacgg ggaacgggcc ccggattcga
     5101 catgcagtgt ttgctcatga tcagttggtc ttgaagcccg ggatctcgcg ccagaagatg
     5161 cggcccttgc cgaggttgga ggacaggttg atctcgcctc gcgcggaaat caccttggtg
     5221 caatccaatg aaccgccgcg gcacgggtcc gccttgtccg ggtcgccgcc gtttccacct
     5281 tcgcccacct gggtgttgcc gatgcgcgcc ttctgatcga ctgctgcaat gctggccagc
     5341 tggtaggtga tcgtgacatc gccctcctcc acatccgtcg ttccgagcag gccgttcggc
     5401 aagccggtga tcgggtcgac ccccagcagg cgtgccaccg gcgtgtccga gcaggcgtcg
     5461 gtggccgcac cggtcttcgg cggcgccgga gacacggtga ccgtgagcac ctggttgttg
     5521 gcgatcgtca ggttgttcag ccccatctcc gagttcacgt tgaagaagaa gtaccagccc
     5581 agcttggtgt tccagtcgat cgcgtcacct gtcaggtagc gattctgggt gtcggtgccc
     5641 acgttgtgga gttcgcgttc ttccaggtcg ctcaggtcgc gcggcagggc gtcttccatg
     5701 tcggtcgcct catcgatgac cgcgaaagcc ggcttgtccc agatgccgaa catgccattc
     5761 gttcgacttc cgttcgggaa gtcgtcgctg gtcaccgact ttccggtgac gacgttcacc
     5821 acgatgccgc cgagacggtg aaagctgaac tccggcgcgc cggtgatggg cagggcggtg
     5881 cctgaagcat ccttggcggt gaagagaggc ttctcgaagt acgacacccg ccaggcgcct
     5941 ggatcggcac tgcggacgtc gaaacgccac aggttgccct ggatgtcgcc cgcgtagatg
     6001 tagtcgggaa tgcggtcgtt gttgacatcg acccaggtcg gttgcgacag cccgttcgca
     6061 ccgttggtcg gcaccacgat cttcttgaac ttgcctcccg cggctgcagc atcaccgtct
     6121 gcgaagacga tgtagagcgc tgcccggccg ctcgtgctgt cgatgccgtt gccgaacagc
     6181 gcagcgaacc gtccgttgtt catccgcgcg atctgtcccg gctgggcggt gaagatgctg
     6241 gtcgtcggtt ccgcgatcat gtaacccatg tcgccctccg ggtcgtcggc atccgtcatc
     6301 tgccatgcga acaccgtcgt ggcgtgcgcc tggtccagtt gcgagccgac cacgctggtc
     6361 gtgtcgtcgg cggttgtctc ggtgcctttg gtgtcgatcg tgatcgtgcc cgcctcagtc
     6421 acgtcgagtg caaagatgcc cttgccgccg cggcccaaag gcgagaag
//
LOCUS       DAOORV010000182         6373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14871_length_6373_cov_11.846470, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000182 DAOORV010000000
VERSION     DAOORV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14871_length_6373_cov_11.846470"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKV56_16385"
     CDS             complement(<1..403)
                     /locus_tag="PKV56_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69438.1"
                     /translation="MSWTSIRLGDVVDVKHGFAFKGEFFADSGRYILLTPGNCHEAGG
                     IKLRGDKEKYYVGEFPAEYILGQGDLLVVMTDLVGTAPVLGGAFVVPEGDRFLHNQRL
                     GLVQVTDTARMHRGFLYHLLNSHSYRAQVRGS"
     gene            complement(400..2514)
                     /locus_tag="PKV56_16390"
     CDS             complement(400..2514)
                     /locus_tag="PKV56_16390"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX69439.1"
                     /translation="MSSSALNELNKIEASLWEAADQLRANSKLTSSEYCMPVLGVIFL
                     RHASNRYDDALAAIQAEQEAGRMPKRPLKQADFKRRRALMLPPQAHYSHLMMLASSPN
                     IGVALVGAMNAIEADFEPLLNQLPKEYDQFDARLLEDLLRAFDTDALRRASGDVFGRI
                     YEYFLMKFAMQGAQDNGEFFTPPSLVQMIVNFIEPDHGVVADLACGSGGMFVQSSHFI
                     EHEGLDTMTRVTFYGQEKTPSTIRLAKMNLAVHGLEGDIRAANTFYEDEHRLAGGKPL
                     WGNCDFMMANPPFNVDMVDATRVKDDRRLPFGLPGVNKAGKVGNANYLWISYFHSYLS
                     PTGRAGFVMSSQASSAGHGEAEVRRKLVETGDVDVMVAIRPNFFYTRTVPCELWFYDR
                     GKPAERRDTVLMLDARQVFRKVTRKIFDFSPEQLANLTAVVWLHRGQTARFEALVAQH
                     LHNAVAAAQACFVVQTEFETVASALTVAANYRALAALVHPYLTYAPAADDAALIAIDQ
                     LRVVTEGVVATHAELVASHVARVGFHAEQLLPLADQAHRLVATLDTAVRELLLLMGDA
                     EQLRQDRELPPWPPRETARSRELNRARKALEEARMAAVDQLKAVRHYQRHAAWLIERF
                     SDSLYRDVQGLCKVVKHAEIEAAGWSLTPGRYVGVAAAVEDEDFDFAESIRDIKDELE
                     TLNRNAGEQAERIQHRLAELLA"
     gene            complement(2546..3958)
                     /locus_tag="PKV56_16395"
     CDS             complement(2546..3958)
                     /locus_tag="PKV56_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69440.1"
                     /translation="MTFLDFGAPTWTSALSGRVRAKGPERATAGEWLKFVLASAEHGI
                     SKDEIQFSGIAHWLLGEHDETDVLTKRQVLAALSFKTVTPKLQMVVDDRFRPSAEWAQ
                     CVARIEPRHHRKLGLFGDAPHSVYVVRYRHRSLGWAVALARHADLMHTERTWWLVLNA
                     KGMRVADQPEYGFNSSQAAMAHASTLLQRQFVKSARARHKPNWERFSLQGLGTYAELL
                     VVLPDWPDTFISPVHFPGVRNLLIHLRTNVCQADDGSRLLFLDEVQSDWHASQVGGTS
                     GAGADPLAADPEQAPFARDWPLLALKFALWWATREGVSGLAWSTPSLHQNRWADYGPP
                     TEVYRKGLPEAAGRLARVLQLELGEVTLSSRRIHGQGRPGEPWRVLTANGIPACRGFA
                     HREQAERFAQLTGAVDRHVAPVLRWQPGRVIQAMPLFGVGDPSMWAVPAKAGAKDPPA
                     GLRRGTSADAGAGAGGGTPS"
     gene            complement(4367..5071)
                     /locus_tag="PKV56_16400"
     CDS             complement(4367..5071)
                     /locus_tag="PKV56_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69441.1"
                     /translation="MGGRGSGRVARLAGKCLAEDALPLDIRRLTRDGVLTPGRSFSWQ
                     WMTNGRTVASIYVKPEAWSLTLSYSFTRHGQPAENINQTIALETTPCTFGGSRSWFRC
                     PACCRRVAVIYGAGRHFACRHCKRLAYGSQNEDEHDRALRRANGIRKELGWVPGVIHG
                     LGPKPPGMHWSTFHRLCHQHDTLASASLMGMAERLGILRGRLQDIGDDVDLDDESVSQ
                     FLAPTDLPQRRPRKRH"
     gene            complement(5217..6071)
                     /locus_tag="PKV56_16405"
     CDS             complement(5217..6071)
                     /locus_tag="PKV56_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69442.1"
                     /translation="MQPAKPETFEVIAFPTAPEAAMTAVAGQGYANVRLNALKHGVLS
                     RHTVLAHEDGEEFAELLAALAAEHQPVGPTEVHLVEELAGAVWRKRRVLQAEGATINR
                     GLLSVSKARHDRPGDNDSPARAALPFDPRMADTRLNSPVELAEAIRETPSETSQRAAE
                     ADDDLRATLRAAEVLRRGGVRAYDRGLRALTPSSAEWFAANLEDGGAEATADSLATFI
                     HNDLLPFCRQMVVEVQSHAAIKSQTLGEGLQAHRLEKLNRYETHLDRKFERSLGILLK
                     LKALRNAV"
     gene            6070..>6373
                     /locus_tag="PKV56_16410"
     CDS             6070..>6373
                     /locus_tag="PKV56_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69443.1"
                     /translation="MSLSIVEAVSTHRDAECLAASQDVLDWLHPAISASDGTAACGSA
                     AAGAFEMLALQAHPPVVALSVAQGQAFSKMLSVLSPGGGKPLRPGREQVNLVVVPTA"
BASE COUNT         1150 a         2085 c         1946 g         1192 t
ORIGIN      
        1 cagaacctcg gacctgcgct cgatagctgt gcgaattgag gaggtggtac aggaacccgc
       61 ggtgcatgcg tgctgtatcc gtgacctgga ccaaacccag cctctgattg tgcagaaagc
      121 ggtcaccttc tgggacaacg aaggccccac ccaagacagg cgctgtgcca accaagtcag
      181 tcatcaccac tagaagatcg ccctgcccaa ggatgtactc ggctggaaac tctccgacgt
      241 agtacttctc cttgtcgcca cggagcttga tgccaccggc ttcatgacag ttccctggcg
      301 ttagcaggat gtatctgcca ctgtccgcga agaactctcc cttgaaagca aaaccgtgct
      361 tgacatccac aacatcgcct aggcgaatcg atgtccaact catgccaaaa gctcggcaag
      421 ccggtgctgg attcgctctg cctgctcgcc agcattccgg ttcagcgttt ccagttcgtc
      481 cttgatgtca cgaatcgatt cagcgaagtc gaagtcttcg tcttctactg ctgccgccac
      541 acccacatac cgccccggcg tcaagctcca acccgccgct tcgatctcgg cgtgcttcac
      601 caccttgcaa agcccctgca cgtcacggta gagagaatcc gagaagcgct cgatgagcca
      661 agccgcgtgg cgttggtagt gccgaaccgc tttcaattga tcgaccgcag ccatgcgcgc
      721 ttcttcaagg gccttgcgcg ctcggttaag ttctcggcta cgcgcagtct cacgcggcgg
      781 ccatggtggt agttcacgat cttgtcgtag ttgttcagcg tcgcccatta ggagcagcaa
      841 ttcgcgaacc gctgtatcta gcgtggccac cagccgatgc gcctggtcgg caagcggcag
      901 caattgctcc gcatggaaac ccacccgggc aacatgcgac gcaaccagtt ctgcgtgcgt
      961 tgcaaccaca ccttccgtga cgacgcgcaa ttggtcgatc gcgatgagag ccgcatcgtc
     1021 agcagccggt gcataggtca gatacggatg caccagcgcc gccagcgcac ggtagttggc
     1081 cgccacggtc aatgctgacg caaccgtttc gaactctgtc tgcactacga aacaggcctg
     1141 cgccgccgct acagcgttgt gcaggtgctg cgccacaagc gcctcgaagc gagctgtctg
     1201 ccctcgatgc agccacacta ccgccgtcag attggccagt tgctcggggc tgaagtcgaa
     1261 gatcttgcgc gtgaccttgc gaaacacctg tcgcgcgtcg agcatcagca cggtgtcacg
     1321 ccgctcggcc ggcttgccac ggtcgtagaa ccacaactca caaggcacgg tgcgcgtgta
     1381 aaagaagttg gggcggatgg ccaccatcac atccacatcg ccggtttcca ccaacttgcg
     1441 ccgcacctcg gcctcgccat gtccggcgct gctggcctgc gacgacatca cgaagccggc
     1501 gcgccccgtc ggcgacagat agctgtggaa gtagctgatc cacaggtagt tggcgttgcc
     1561 caccttgccg gccttgttca cccccggcag gccaaagggc aggcggcggt cgtccttcac
     1621 acgggtggcg tccaccatgt ccacgttgaa cggcgggttg gccatcatga agtcgcagtt
     1681 gccccacagc ggcttgccgc cggccaggcg gtgctcgtct tcgtagaagg tgttggcggc
     1741 acggatgtcg ccctccagcc cgtgcaccgc caggttcatc ttggccaggc ggatggtgga
     1801 cggcgtcttc tcctggccgt agaaggtgac acgtgtcatc gtgtccagcc cttcatgctc
     1861 gatgaagtgg ctgctctgca cgaacatgcc ccccgacccg caggccaggt cagctaccac
     1921 gccatggtcg ggttcgatga agttgacgat catctgcacc agcgatggcg gcgtgaagaa
     1981 ctcgccgttg tcctgagcgc cttgcatcgc gaacttcatc aggaagtact cgtagatgcg
     2041 gccgaatacg tcgcccgagg cgcggcgcag cgcgtcggtg tcaaaggcac gcagcaggtc
     2101 ttccagcagc cgggcgtcga actggtcgta ctccttgggc aactggttca gcagcggctc
     2161 gaagtctgct tcgatggcat tcatcgcccc caccagggcg acgccgatgt tggggctgct
     2221 ggccagcatc atcaggtggc tgtaatgcgc ctggggcggc agcatcagcg cacggcggcg
     2281 cttgaagtcg gcctgcttca gcggccgctt cggcatgcgg ccggcctctt gttcagcctg
     2341 gatggcagcc agtgcgtcgt cgtagcggtt gctggcgtgg cgcaggaaga tcacccccag
     2401 aaccggcatg cagtattcgc tgctggtcag cttggagttg gcacgcaact ggtctgccgc
     2461 ctcccacagg ctggcttcga tcttgttcag ttcattcagg gcgctgctgc tcatgggtcc
     2521 accggagatc tgcgttgatt ctcggtcagc ttggcgtgcc tcccccggca ccggcacctg
     2581 catcggccga cgtgccacgc cgcaggccag caggtgggtc cttggcccct gccttcgccg
     2641 gcaccgccca catggaggga tcgcccacgc cgaagagcgg catcgcctgg atcacccgtc
     2701 caggctgcca gcgcaacacc ggcgccacat ggcggtcgac agccccagtc aactgagcga
     2761 agcgctcggc ctgctctctg tgtgcaaagc cgcggcaggc cgggatgccg ttggctgtga
     2821 gcacacgcca gggttcaccg ggcctgcctt ggccatggat ccgcctgctc gacagcgtca
     2881 cctcgccgag ttcaagctgc agcacgcggg ccagtcgacc ggcggcctcg ggcaaaccct
     2941 ttcggtagac ctctgtgggc ggcccgtagt cggcccaacg gttctggtgc aaagaggggg
     3001 tactccacgc caatcccgat accccctccc gggtagccca ccacagcgca aacttcaggg
     3061 ccagcagtgg ccagtcgcgc gcgaatggcg cctgctccgg gtcggccgcc aacgggtctg
     3121 cccccgcacc gcttgtccca ccaacttggc tggcatgcca atcgctctga acctcatcga
     3181 ggaacaggag tcggctgccg tcgtctgcct ggcaaacgtt ggtccgcaag tggatcagca
     3241 ggttgcgcac accagggaag tgcaccgggc tgatgaaggt gtcgggccaa tcaggcagca
     3301 cgactagcaa ttccgcatac gttcccaggc cctgcaggct gaagcgctcc cagttcggct
     3361 tgtgccgcgc gcgtgctgac ttgacgaact gccgttgcaa cagggtcgag gcatgcgcca
     3421 tcgccgcctg gctgctgttg aagccgtact cgggttgatc ggcaacacgc atgcccttgg
     3481 cgttcagcac cagccaccag gtgcgttccg tgtgcatgag gtcggcatgc cgggccaacg
     3541 ccacggccca accgagtgag cgatggcgat accggacgac atagaccgag tggggcgcgt
     3601 cgccgaacag gcccagcttg cggtggtgcc ggggttcgat ccgtgccacg cactgcgccc
     3661 actcagccga cgggcggaag cgatcgtcca ccaccatctg cagctttggc gtgactgtct
     3721 tgaacgacaa agccgccagc acctggcgct tggtcaaaac gtcggtttcg tcgtgttcac
     3781 ccagcagcca atgggcgatt cccgaaaact ggatctcatc cttgctgatg ccgtgctcgg
     3841 cagaggccag cacaaacttc agccattcgc ctgcggtggc gcgttcaggc cccttggcac
     3901 gcacccgacc cgacagtgcg gatgtccatg taggcgcacc gaagtccagg aaggtcaagg
     3961 gctcgcctca tcggttgtgc agcgtgtcgg tgagcggctg acgatggcaa tggcaatgtc
     4021 caacagggag cgctggcgcg cttaaatcat tcaccttgag atttcaattg cagccacggc
     4081 tcaattgtgg ggtccggcag cgcggtcccg ccagggtctt ggccctcgcg ccacacagcg
     4141 cgccgcccaa cttcgggacg aatgcggcgg tgtaggccaa gctggaggca tgaaagtgcc
     4201 attgacggct tgaccaatct tgactctgca attgaccgct cccagagcga gagccgtggc
     4261 aaaactgccc tgactctcgc cagccggcaa acccggcggc atagcctgaa ggcagcgttg
     4321 cagagttgcc tccaagttgc ccgcgcgccg acttgcctcc tcagggtcaa tgacgtttcc
     4381 tgggtcgtcg ctgcggtagg tcggtgggcg ccagaaattg cgaaacagat tcgtcatcca
     4441 gatcgacatc gtctccgatg tcttggaggc gaccgcgcag gatccccaat cgctcagcca
     4501 ttcccatgag cgatgcggaa gccagggtgt cgtgctggtg gcacaagcga tgaaacgtag
     4561 accaatgcat gcccggtggc ttgggaccca gcccatggat cacgcctggt acccagccca
     4621 gttccttgcg gatgccgttg gcacggcgca gtgcgcgatc gtgttcatct tcgttttgac
     4681 tgccataggc cagccgcttg cagtggcggc aggcaaagtg ccgccctgcg ccgtagatca
     4741 cggccacacg ccgacagcat gccgggcagc ggaaccatga ccggctgccg ccgaaggtgc
     4801 atggcgttgt ttccagcgcg atcgtctggt tgatgttctc ggccggttga ccgtggcgcg
     4861 tgaagctgta cgacaaagtc agagaccacg cctccggctt gacgtagatt gacgccaccg
     4921 tcctgccatt tgtcatccac tgccaactga aggaccggcc cggtgtcagt acaccgtcgc
     4981 gggtcaggcg ccggatgtcc aaaggcaaag cgtcctcagc gaggcacttg ccggcgaggc
     5041 gggcgacacg gccgctaccg cgcccaccca tggcgggctc ccgtttcttc cgaaatcatc
     5101 aggcaagtct gcagagtctt tggaacgaca aagaccgcac gaggcacaca agccgttgtc
     5161 gagattgaag ttttcggcat caggcggact tcgtcggcat gctcagggtc actgcttcaa
     5221 actgcatttc gaagcgcttt cagcttcaga agaatcccca gggaccgctc gaacttgcgg
     5281 tcaagatggg tttcgtagcg gttcagcttc tccagccggt gcgcctgcag cccttcaccc
     5341 aaggtttgcg acttgatcgc cgcatggctt tgcacctcca ccaccatctg gcgacagaac
     5401 ggaagcaggt cgttgtggat gaatgtggca aggctgtctg cagtcgcctc cgctccacca
     5461 tcctccaagt tggcggcgaa ccactcggcg ctgctcggcg tcaacgcgcg caagccgcgg
     5521 tcataggcgc ggacgccgcc acgtcgcagc acctctgcag cccgaagggt tgccctcaga
     5581 tcatcatccg cttcggcggc gcgctggctg gtttccgatg gcgtctcccg gatggcctca
     5641 gccaactcca cgggtgagtt cagccgggtg tcggccatcc tgggatcgaa gggcagcgcg
     5701 gcgcgggccg ggctgtcgtt gtcaccaggc cggtcatggc gagcctttga caccgacagc
     5761 aagccccgat tgatcgttgc accctcggcc tgcaggacgc ggcgcttgcg ccagacggcg
     5821 ccggccagct cttcgaccag atgcacctcg gtcggaccga cgggttgatg ctcagccgcc
     5881 agagccgcca gcagttcagc gaactcttcg ccgtcctcgt gcgccagcac cgtgtgccgg
     5941 ctaagcacgc catgcttgag ggcgttgagc cgaacgttgg catagccctg gccggcgacg
     6001 gccgtcatgg ccgcttctgg tgccgtcgga aaagcgatca cttcgaaggt ttcgggtttg
     6061 gctggctgca tgtcactttc cattgttgaa gccgtgagca ctcacaggga tgcggaatgc
     6121 ctcgcagcga gtcaagatgt tctggactgg ctccatccgg cgatcagcgc cagcgatggc
     6181 accgcggcgt gcggcagcgc agcggccggt gcgttcgaga tgctggcact ccaggcacat
     6241 ccgccggtcg tcgccttgtc tgtcgcgcag ggtcaggcgt tcagcaagat gctcagcgtc
     6301 ctttcgcccg gcggcggcaa accgctccgc cctggccgtg aacaagtcaa tctcgttgtc
     6361 gtcccaaccg ccg
//
LOCUS       DAOORV010000183         6362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_14908_length_6362_cov_7.709212, whole genome shotgun sequence.
ACCESSION   DAOORV010000183 DAOORV010000000
VERSION     DAOORV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14908_length_6362_cov_7.709212"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKV56_16415"
     CDS             complement(<1..205)
                     /locus_tag="PKV56_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOX69444.1"
                     /translation="MTYCVGIKLDAGLVFLSDSRTNAGLDQISTFRKMMVYEKPDDRF
                     MVMLSAGNLSISQSVREILQVEKI"
     gene            complement(202..2847)
                     /gene="mutS"
                     /locus_tag="PKV56_16420"
     CDS             complement(202..2847)
                     /gene="mutS"
                     /locus_tag="PKV56_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOX69445.1"
                     /translation="MPPESSSSISPPVDLKAHTPMMQQYLRIKAEHPDTLVFYRMGDF
                     YELFFDDARRANRLLDITLTTRGQSGGEPVVMAGVPVHSFENYLARLIRLGEPVAICE
                     QVGDVATAKGPVERKVVRVVTPGTLTDTELMSDKADALLLALAQHTHRGKTVHGLAWL
                     ALSSGELGLTECAEHELPGWLARLNAAEVLVEREMVPTALSAVRAAVTHRPDWQFDAA
                     LGARKLCEQLRVASLAGFNAGEMTVAHAAAAALLSYAEHTQGQALAHVRRLQVERTSE
                     LIDLPPTTHRNLELTQTLRGEDAPTLLSLLDTCRTGMGSRALRGWMTRPLRDRGVAIQ
                     RHEAIEATIAQGGEALRSALRQVSDVERITARIALRQVRPRELAGLRATLRALPEVRS
                     TVPASASALLETLCVALSPDASIEVLLTTTLAEEPAALVRDGGVIATGCDATLDELRS
                     IAQNCDAFLLDLESRERTRTGIANLRVQFNRVHGFYIEVTQGQLDKVPADYQRRQTLK
                     NAERYITPELKAFEDKALSAQERSLAREKLLYDQLVDTLQSHVPQLCDLARALAALDA
                     LAALAERASTLNWCRPQFVREPCIDIQGGRHPVVEARLQETGAGHFMANDCRLDATRK
                     MLVITGPNMGGKSTFMRQVALICLLAAMGSFVPAIACRLGPMDAIHTRIGAADDLANA
                     QSTFMVEMTEAAAILHGATAQSLVLMDEIGRGTSTFDGLALAGAIASHLHDRNNAFTL
                     FATHYFELTDFPTKHERALNVHVSAAETRTKHGDDIVFLHDIQPGPASRSYGVQVARL
                     AGMPTSLVRQARTTLEALESQQKSGQAQIDLFAPPTSIAAPESSAVERALAQINPDAL
                     TPKQALDALYRLQALQHPPESDTAP"
     gene            complement(3012..4475)
                     /locus_tag="PKV56_16425"
     CDS             complement(3012..4475)
                     /locus_tag="PKV56_16425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOX69446.1"
                     /translation="MDKTSPLVHPERLGKYQVTGVLGEGAMGVVYKGFDPGIQRAVAL
                     KTVRRQLLQDNDHGPSVVARFRNEAQAAGRLSHPGIVAVYDYGDEGDVAYIAMEYVEG
                     NTLQRYIAGQVSFSEGDIISVVVQLLDALEHAHGQGVWHRDIKPSNILMTRDGRLKIA
                     DFGIARIESAQLTLVHSVIGTPSYMAPEQFQGHGIDRRVDIYATGALLYQLLVGRPPF
                     VGSTDSLAYRVVHEAPVLPSAVEGYRWGAQYDPVLAVALSKDPGLRYPTAQAFKRALI
                     SVTDNPVSATVSDETIVAVPASHAASPVGSSTGREGSAAGSRPSASTGGTPSNWEPAL
                     LAQVEASLARHVGPLASVLVRRTARECTELSTLVLRLADQVTNVQAREAFLSQSARHL
                     QTRSGASSQHPDPGLRSAGGRAGAEAGREITPLSESFIAQGTRLLAAHVGPIASVLAK
                     RAAARGGGKEAFVQALTEAVTDAAARERLRTELSRLG"
     gene            complement(4479..5351)
                     /locus_tag="PKV56_16430"
     CDS             complement(4479..5351)
                     /locus_tag="PKV56_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOX69447.1"
                     /translation="MKLLVKFNLLFLTVMLLGLLVSGYVTRSLLQDNAKEETLNNARL
                     LIEKALAVRGYTSSQITKLLATQMKYEFLPQSVPSYSAVEVLTTLQSKYPQFSYKEAT
                     LNPTNPRDRATGWEVDIVNQFRSGSELKEVIGQRDTPTGPSLYIARPLRITNPACLGC
                     HSSVEAAPRTLVERYGPANGFGWTLNEVVGAQIVSVPMDVPLQRADRSFQVFMTAQLA
                     VFIAVGVVLNLMIWLVIVRPVTRLSSLADRVSQGEMDAPEFDNRSSDEIGNLAASFNR
                     MRTSVVQAMRMLDN"
     gene            complement(5379..6206)
                     /locus_tag="PKV56_16435"
     CDS             complement(5379..6206)
                     /locus_tag="PKV56_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69448.1"
                     /translation="MRTSLLAVALTTMVNGHCRAEGWMLTLLDGEAVVIDGARRLTAG
                     PGLRVSPGALVETSATTALVRFEGPDQATIDLGPDTRAMLAPDNATARGGRPPAVYLQ
                     QGSVKLTSQEGSTFPGLVAPGLELLPFSGSAVVQVGKGDRAVFAEAARLDVGERHSGG
                     VPRFLKAGEFYSGDGKRAGIVAERPTADWLKQVPRAFRDPLPLRAAALRDKPFEAPPL
                     PGPTYEQLARWLGAEPYVRRDFVSRFKPLARDAAFRRALQSHLASHPEWAVVLSPDR"
BASE COUNT          974 a         2127 c         2168 g         1093 t
ORIGIN      
        1 cgatcttctc gacctgcagg atctcgcgca ccgactggct gatcgacagg ttgccggcgg
       61 acagcatcac catgaagcgg tcgtccggct tctcgtagac catcatcttg cggaaggtgc
      121 tgatctggtc gagtccggcg ttggtgcgcg agtcggacag gaagaccagc ccggcgtcga
      181 gtttgatgcc tacgcaatag gtcatggtgc ggtgtcactt tctggtggat gttgcagcgc
      241 ctgcaatcga tacagcgcgt cgagggcctg tttcggggtg agggcgtcgg ggttgatctg
      301 ggccagcgcc cgttcgaccg ccgatgattc gggtgcagcg atggaagtcg gcggggcaaa
      361 caggtcgatc tgcgcctgtc ccgatttttg ctgggattcg agcgcttcga gcgtggtgcg
      421 agcctggcgt accagcgacg tcggcatgcc ggccaaccgc gccacctgca cgccgtagct
      481 gcgactcgcc gggccgggct ggatgtcgtg caggaagacg atgtcgtcgc cgtgcttggt
      541 gcgggtctct gcggcagaga catgcacgtt gagcgcgcgc tcgtgcttcg tcgggaagtc
      601 cgtcaactcg aagtagtgcg tcgcgaacag cgtgaacgcg ttgttgcggt cgtgcaggtg
      661 gctggcgatc gcgccggcga gtgccaggcc gtcgaaggtc gaggtgccgc ggccgatctc
      721 gtccatcagc accagcgatt gcgcggtggc accgtgcagg atggcggcgg cctcggtcat
      781 ctccaccatg aacgtcgact gtgcattggc caggtcgtcg gcggcgccga tgcgggtgtg
      841 gatcgcgtcc atcggcccga gccggcaggc gatggcgggc acgaacgaac ccatggctgc
      901 caacaggcaa atgagcgcga cctgcctcat gaaggtgctc ttgccgccca tgttcggccc
      961 ggtgatcacc agcatcttgc gcgtcgcgtc gagccggcag tcgttggcca tgaaatggcc
     1021 tgcgccggtt tcctgcaaac gggcctcgac cacgggatgg cggccaccct ggatgtcgat
     1081 gcacggctcg cgaacgaact gcggccggca ccagttcagc gtggaggcac gttcggccag
     1141 cgcagccagc gcatcgagcg cggcgagcgc ccgcgccaga tcacacagtt gcgggacgtg
     1201 tgactgcagt gtgtctacca gctggtcgta gagcagcttc tcgcgcgcca gggagcgttc
     1261 ctgcgcggac agcgccttgt cctcgaaggc cttcaactcc ggcgtgatgt agcgctcggc
     1321 gttcttcagg gtctggcggc gctggtagtc ggctggcacc ttgtccagct gaccctgtgt
     1381 gacctcgatg tagaagccgt gcacccggtt gaactgcacc cgcaggttgg caatgccggt
     1441 gcgtgtgcgc tcgcgcgatt cgagatcgag caggaaggca tcgcagttct gcgcgatcga
     1501 cctcagctcg tcgagcgtgg cgtcgcagcc ggtggcgatc acaccgccgt cgcgcaccag
     1561 tgcggccggc tcctccgcca gcgtcgtcgt cagcaacacc tcgatcgagg cgtcgggact
     1621 caaggccaca cacagcgtct ccagcagcgc gctggcgctg gcgggcaccg tcgaacggac
     1681 ctccggcagt gcacgcagcg tggcgcgcag tccggccagc tcgcgcgggc gtacctggcg
     1741 aagcgcgatg cgcgcggtga tccgctcgac atcgctcacc tgtcgcaacg cggagcgcaa
     1801 ggcctctccg ccctgggcga tcgtcgcctc gatggcctca tggcgctgga ttgctacgcc
     1861 gcggtcgcgc agtggccggg tcatccaacc gcgcagcgcc cggctgccca tgccggtgcg
     1921 gcaggtgtcg agcagcgaca gcagcgtcgg tgcgtcctcg ccgcgcaacg tctgcgtcag
     1981 ctcgagattg cggtgcgtcg tcggtggcag gtcgatcagt tcgctcgtgc gctcgacctg
     2041 aaggcgtcgc acgtgcgcaa gcgcctgtcc ctgcgtgtgc tcggcataac tcaggagcgc
     2101 cgctgcagcg gcatgagcca cagtcatttc gccggcgttg aagccggcga ggctggccac
     2161 ccgcaactgc tcgcagagct tgcgagcgcc tagcgcggcg tcgaactgcc agtccggccg
     2221 gtgcgtcacc gcggcgcgaa cggccgacag cgccgtcgga accatctcgc gctcgaccag
     2281 cacctcggct gcattcaggc gggccagcca cccgggcaat tcgtgctccg cacattcggt
     2341 caggccgagc tcgccgctgc tcagggccag ccaggccagg ccgtgcactg tcttgccacg
     2401 atgcgtgtgc tgcgccagcg ccagcagcag agcgtccgcc ttgtccgaca tcagctcggt
     2461 gtcggtcagc gtgcccggcg tgaccacacg caccaccttg cgctcgaccg gccccttggc
     2521 cgtggcgacg tcgccgacct gttcgcagat cgctacgggc tcgccgagcc tgatcagccg
     2581 ggccaggtaa ttctcgaacg aatgcaccgg aacgcccgcc atcaccaccg gctcgccgcc
     2641 gctctgtccg cgcgtggtca acgtgatgtc gagcagccgg ttcgcacgcc gcgcgtcgtc
     2701 gaagaacagc tcgtagaagt cgcccatccg gtagaacacg agcgtgtccg ggtgctcggc
     2761 cttgatgcgc aggtactgct gcatcatggg tgtgtgggcc ttcaggtcca ccggtgggct
     2821 gatgctgctg ctggattcgg ggggcatcgg cgatccgggg ggcgttttcg tggcggggcg
     2881 gctatttgac cgggacggat attaggggac ggaggccgcg agacgattcc tggcggccag
     2941 gttgactccg catgtctggg ccaccgcttg catttcgact cggcatcatc gagccaccat
     3001 cggcgcgcgc gtcagcccag gcgggacagc tcggtcctca ggcgctctcg cgcggcggcg
     3061 tcggtcacgg cttcggtcag ggcctgcacg aacgcctcct ttccgccgcc gcgggccgcg
     3121 gcccgtttgg ccagaacgct ggcgatgggc ccgacatggg ctgccagcaa gcgtgtgccc
     3181 tgggcgatga agctttcgct caacggtgtg atctcccgtc cggcctcagc ccccgcacgg
     3241 ccgccggccg accgcaatcc ggggtccggg tgctggctgg acgcgccgct gcgcgtctgc
     3301 agatgcctgg cgctctggga caggaacgct tcgcgcgcct ggacgttggt gacctggtcg
     3361 gccaggcgca gcaccagagt cgacagttcg gtgcattcac gagccgttcg gcgcaccagg
     3421 acgctggcca agggcccgac atggcgcgcc aggctggcct cgacctgtgc cagcaacgcg
     3481 ggctcccagt tcgagggtgt gccgccggtg gatgcgctcg gccggctgcc tgcagcgctg
     3541 ccttcgcgcc ccgtactcga gcccacgggc gacgccgcat ggctcgccgg cacggcgacg
     3601 atcgtttcat ccgagacggt ggcggagacg gggttgtccg tgacggagat cagcgctcgc
     3661 ttgaaggcct gcgcagtggg atagcgcagg ccgggatcct tggacaacgc caccgcaagc
     3721 acggggtcgt actgcgcacc ccagcggtag ccctcgacgg ccgagggcag caccggtgcc
     3781 tcatgcacca cgcggtaggc cagggaatcg gtgctgccga cgaacggtgg cctgcccacc
     3841 agcaactggt acagcagcgc accggtggcg tagatgtcca cccggcgatc gatgccgtgg
     3901 ccctggaact gctcgggtgc catgtagctg ggcgtgccga tgacgctgtg aaccagcgtc
     3961 aactgcgccg attcgatgcg cgcaatgccg aagtcggcga tcttcaaccg accgtcgcga
     4021 gtcatcagga tgttggatgg cttgatgtcg cggtgccaca cgccctggcc gtgcgcatgc
     4081 tccagtgcat cgagcaactg aaccaccacg ctgatgatgt cgccctcgct gaaactcacc
     4141 tgaccggcga tgtagcgctg cagggtattg ccctcgacgt attccatcgc gatgtaggcc
     4201 acgtcgcctt cgtcgccgta gtcgtacacg gcgacgatgc ccgggtgcga cagccgtccg
     4261 gccgcctgcg cctcgttgcg gaagcgcgca accacgctcg ggccgtggtc gttgtcctgc
     4321 agcagctgcc ggcgcacggt cttcagtgcc accgcgcgct ggatcccggg gtcgaaaccc
     4381 ttgtagacca cccccatcgc gccctcgccg aggacgccgg tgacctggta cttgccgagg
     4441 cgttcggggt gaacgagagg cgaggtcttg tccaccggtc agttgtccag cattcgcatg
     4501 gcctggacca cgctggtgcg catgcggttg aacgatgcgg ccaggttgcc gatctcgtca
     4561 ctgctgcggt tgtcgaactc cggggcgtcc atctcgccct ggctgacgcg gtcggccagc
     4621 gacgacagcc gggtcaccgg gcgaacgatc accagccaga tcatcaggtt cagcacgacc
     4681 ccgacggcaa tgaacactgc gagttgtgcc gtcatgaaca cctggaacga gcggtcggcg
     4741 cgctgcagcg gaacatccat cggcaccgag acgatctgtg cgccgaccac ttcgttgagg
     4801 gtccatccga agccgttggc cggtccgtag cgctccacca gtgtcctggg cgccgcctcg
     4861 acgctgctgt ggcaccccag gcaggccggg ttggtgatgc gcagcggtcg ggcgatgtac
     4921 agcgatgggc ccgtcggggt gtcgcgctgg ccgatcactt ccttgagctc gctgccgctg
     4981 cggaactggt tgacgatgtc gacctcccag ccggtggcgc ggtcacgggg gttggtcggg
     5041 ttcagcgttg cttccttgta cgagaactgc ggatacttgg actgcagcgt cgtcagcacc
     5101 tcgaccgccg agtagctggg aaccgactgg ggaaggaact cgtacttcat ctgggtcgcc
     5161 agcagcttgg tgatctggct cgaggtgtag ccccgcacgg ccagcgcttt ctcgatcagc
     5221 agccgcgcat tgttcagcgt ttcctccttg gcgttgtcct gcagcaggct gcgggtgacg
     5281 tatccgctga ccagcagccc gagcagcatc acggtcagga acaacaggtt gaacttcacc
     5341 agcagcttca tgcgtacgtt cctcgtgaat cggtattttc agcggtccgg gctgagcacc
     5401 acggcccact cggggtggga cgccagatgc gattgcagcg cgcgccggaa tgcggcatca
     5461 cgcgccaggg gcttgaagcg cgacacgaag tcgcgccgta catagggctc ggcgccgagc
     5521 cagcgggcga gctgttcgta ggtcgggccg ggcaggggcg gcgcttcgaa cggcttgtca
     5581 cgcaaggccg cagcgcgcag cggcagcgga tcgcgaaagg cgcgaggcac ctgcttcagc
     5641 cagtctgcgg tcggccgttc ggcgacgatg cctgcgcgtt ttccgtctcc gctgtagaac
     5701 tcaccggcct tcaggaacct cggcacgccg ccggagtggc gctcgccgac atccagccgg
     5761 gcggcttctg cgaagacggc tcgatcgcct ttgccaacct gcaccacggc gctgcccgaa
     5821 aacggcagca gttcgagtcc cggcgcaacg aggcccggga aggtcgaccc ttcttggctg
     5881 gtcagcttca ccgagccctg ctgaaggtac acggccggcg ggcggccgcc gcgcgctgtc
     5941 gcgttgtccg gcgcgagcat ggcccgggtg tcgggaccga ggtcgatcgt cgcctggtcc
     6001 ggcccttcga agcggaccag ggccgtcgtg gcgctcgtct ccaccagcgc cccgggactc
     6061 acgcgcaggc cgggaccggc ggtcagtcgg cgtgcaccgt cgatgaccac ggcctcgcca
     6121 tccagcagcg tcagcatcca gccttccgcc cggcagtgac cgttgaccat cgtggtcagc
     6181 gccacggcca gcaggctggt acgcaacctg acgctcgtcc accatctgcc tctacccgac
     6241 gaccacatcg cttgccaccc cattccctcg ccacgccccg agcatcgcat ccgaacttgc
     6301 aacctgcaga tgcaagcatc gaacatgcct tcgctgctga gatgatctcg gtgcgggcgc
     6361 ga
//
LOCUS       DAOORV010000184         6305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15063_length_6305_cov_5.789600, whole genome shotgun sequence.
ACCESSION   DAOORV010000184 DAOORV010000000
VERSION     DAOORV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_6305_cov_5.789600"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="aceE"
                     /locus_tag="PKV56_16440"
     CDS             complement(<1..663)
                     /gene="aceE"
                     /locus_tag="PKV56_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747281.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOX69449.1"
                     /translation="MSVMPEPIVGAADAHASDTDAQETHEWLDALSAVIGAEGPERAH
                     YLLEQLLEHARQNSIDMPFSANTGYVNTIEPEDEERNPGNLELEGRLRACMRWNAMAM
                     VVKANRLHPADGGDLGGHISSFASLAHMLATGFNHFWHADHEGHGGDLLYIQGHSAPG
                     IYARAFLEGRLTEEQLLNFRQEGDGKGLSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQ
                     ARF"
     gene            857..3406
                     /locus_tag="PKV56_16445"
     CDS             857..3406
                     /locus_tag="PKV56_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX69450.1"
                     /translation="MDSPSRSNLASRMPLPGTRRLFGRWWRRQSPAHQDRYATLAPLA
                     SVLLFLAAIISAFWYLRNEEFERAGESVRRDTELAQQQIRLRLIENEESLGRIAQDLV
                     LRDPDRSLFQRNAADLIRERPEISRVYWLAANRSVVSTQSANGYQVDNEPGAVVLEHG
                     MSLPKEGSDNPAEAAFVSARKNRGTAYSSAFADGAGANVVQVHVPLLVRNVFLGMVVA
                     EYSTDSLIRFLVPPDVSNRHVISLLTPKGRTLSSTVMPMPGQEAPKATIVHDVPVLPS
                     GDALMLRGQGYRTSVGLIGNTLFWMVVALSVLTVWTLFGTWRHVRRRSQMQSALVSEA
                     NFRRAMENSMLTGMRAMDMEGRITYVNPAFCAMTGFGESELIGRMPPFPYWPPDRHDE
                     INRLLQQELQGRSPSGGIEVKVMRKDGEMFDARMYVSPLIDPKGIQTGWMTSMTNITE
                     AKRIRDQLSASHERFTTVLEGLDAAVSVLSVQQGELLFANRSYRLWFGADALGHALLA
                     GDDIDAMPAEPEDSVESMGGLPTQELTEIGSDPREVLIEPLNKWFDVRVRYLQWTDGR
                     LAQMLIATDVTSRRNAETLAASQSEKAQVTSRLVTMGEMASSVAHELNQPLTAIANYC
                     NGMVSRVKNDAIEKEDLIAALEKTSKQAQRAGQIIHRIRAFVKRSEPQRQEAQAQTII
                     DDAVDLASIELRRRKVSIQTYVAQRLPPLLVDPILIEQVVLNLLKNAAEAIDSADLPA
                     ARRRIELRVVPKRNAEEGHVIEFSVTDMGPGIRPEVLARLYEAFFSTKAEGLGIGLSL
                     CRSIIESHRGRIRAENLYNAGSVSGCCFAFTLPVESSRIEALPSPNPVVTS"
     gene            3403..4026
                     /locus_tag="PKV56_16450"
     CDS             3403..4026
                     /locus_tag="PKV56_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX69451.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVKCFDSSESFL
                     SRFDPREVACLIADIRMDGMSGLELQDRLIERKSPLPIVFITGHGDVPMAVTTMKKGA
                     MDFIEKPFKEEELVGLVERMLDEARSAFSQHQEAASRDALLSRLTTREGQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGAQGKS"
     gene            4253..>6305
                     /locus_tag="PKV56_16455"
     CDS             4253..>6305
                     /locus_tag="PKV56_16455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOX69452.1"
                     /translation="MTNPLLRADALPAFDEIRPEHVQPAIDTLLATTDAALERAVGPD
                     TPAEYDAVSSVLDTAAEHLRFVWGAIGHLNAVADTPALRAAYNDALPKVTEQSSRHAA
                     DERLYAKYKAIAKNAACVGSASSLTPARKQALSNAMRDFVLAGAELQGEAKQRFQRLQ
                     ELQADLSQKFSEHVLDATDGYACYASTEELAGVPADAREASRAAAAAEGKAGHKVTLH
                     FPSYFPVMQYGENRALRERLYTANATRASEMGPPDQDNSEVMRELIRLRQEEARLLGY
                     PSYADVSLVPKMAASPRQVMDFLRDLAGRARPHAQRDLAELREFARTELGIDDLQAWD
                     VPFASEKLKEARYAFSDQEVKQYFTEPKVLEGLFRIIETLFEVAIRPDSAPVWHPSVR
                     FFRIERPAEPGSDGPAELVGQFYLDPYARPGKRPGAWMDEVRGRWARPEGRLQTPVAQ
                     LVCNFTPPLPGKAALLSHDDVTTLFHEFGHGLHHMLTQVDDIGVAGISGVEWDAVELP
                     SQFMENFCWEWDVVKRLTAHADTGEPLPRELFDRMLAAKNFQSGLQTLRQVEFSLFDM
                     RLHAEPGSEDRIEALLEEVRDEVSVFKPPAFNRFQHSFSHIFAGGYSAGYYSYKWAEV
                     LSADAWAAFEETSQTGGVLNVETGRRYRQTVLEAGGSRPAMESFKAFRGRSGSGHALV
                     TQ"
BASE COUNT         1153 a         2026 c         2019 g         1107 t
ORIGIN      
        1 gaagcgcgcc tggtagatcg ccatcaacgg tcccagaccc atcgacaccg tcgggaactg
       61 ccagaactcg ggcatcagct tgggatgcgg gtagctgctg agccccttcc catcgccttc
      121 ctgacggaag ttcagcagtt gctcttcggt cagccggccc tccaggaagg ctcgggcgta
      181 gatgcccggc gcactgtgtc cctggatgta cagcaggtca ccgccgtgcc cctcatggtc
      241 ggcatgccag aaatgattga acccggtggc cagcatgtgc gccagcgagg cgaagctgct
      301 gatgtgtccc ccgagatcgc cgccatccgc cgggtgcagt cgattcgcct tgaccaccat
      361 cgccatcgcg ttccagcgca tgcaggcgcg aagacgacct tccagttcca ggttgccggg
      421 attgcgctct tcgtcctcgg gctcgatcgt gttgacgtag ccggtgttgg ccgagaacgg
      481 catgtcgatg ctgttctgcc gagcatgctc gagcagttgc tcgagcaggt agtgcgcacg
      541 ttcgggccct tcggcaccga tcacggcgga cagcgcgtcg agccattcgt gcgtttcctg
      601 cgcgtcggtg tcgctggcgt gggcatcggc tgcaccgacg attggttcag gcatcacgga
      661 catggcgtct ccggaaagta gtgctttcaa cgtgcaacga gtttctcaca actcggccaa
      721 acatttcgaa ttacgcaatg cgatttcata ttatggtttt aatgttcgac tgagggtcac
      781 tcctgcctcg tctcgttcct ggacagcgaa ccgcttcgac gtctaccgcc ctcgccgccg
      841 actcgataat ccggtgatgg acagtccttc gagaagcaat ctggcctccc gcatgccctt
      901 gcccggcacg cggcgcctgt tcgggcgttg gtggcgacgc cagtcaccgg cgcaccagga
      961 tcgctacgcc acgctggcac cgcttgcgtc ggtgcttctc ttcctcgcgg ccatcatctc
     1021 ggcgttctgg tacctgcgca atgaggaatt cgagcgcgca ggtgaatcgg ttcgccgcga
     1081 caccgagctc gcccagcagc aaatccggct caggttgatc gagaacgagg aaagcctggg
     1141 tcgcatcgct caggatctgg tcttgcgcga ccccgaccgc agcctgttcc agcgcaacgc
     1201 cgccgacctg atccgcgagc gcccggagat cagccgggtc tactggctgg cggccaaccg
     1261 cagcgtggtc tcgacccagt cggccaacgg ctaccaggtg gacaacgagc cgggcgctgt
     1321 cgtcctcgag cacggcatgt cgttgccgaa ggaagggtct gacaacccgg cggaagctgc
     1381 cttcgtcagc gcccgcaaga accgtggaac agcgtattcg agcgccttcg ccgacggggc
     1441 cggagccaac gtcgtgcagg tgcacgtgcc gctgctggtg cgcaacgtgt tcctggggat
     1501 ggtggtggcc gagtactcga cggacagcct gatccgcttc ctggtgccgc ccgatgtgag
     1561 caaccgtcat gtgatcagcc tgctcacacc gaaaggtcgc acgctgtcga gcaccgtgat
     1621 gccgatgccc ggtcaggagg cgcccaaggc cacgatcgtg cacgacgtgc cggtgctgcc
     1681 gtccggcgat gccctgatgt tgcgcggaca gggttatcgc acctcggtcg gcctgatcgg
     1741 caacacactg ttctggatgg tggtcgcgct gagtgtgctg acggtctgga ccctgttcgg
     1801 cacctggcga cacgtgaggc ggcgatcgca gatgcagagc gccttggtgt ccgaggcgaa
     1861 cttccgccgc gccatggaga actccatgct gaccggcatg cgagccatgg acatggaggg
     1921 ccggatcact tacgtgaacc ctgccttctg cgcgatgacg ggcttcggag aatccgagct
     1981 catcggacgc atgccgccgt tcccgtactg gccgccggat cgtcacgacg agatcaatcg
     2041 actgctgcag caggagctgc agggacgaag cccctcaggc ggcatcgagg tcaaggtgat
     2101 gcgcaaggat ggcgagatgt tcgacgcacg catgtacgtg tcgccgctga tcgatccgaa
     2161 gggaatccag acgggctgga tgacctcgat gaccaacatc accgaggcca agcgcatccg
     2221 cgaccagttg tctgcgtctc acgaacgctt cacgaccgtc cttgaagggc tcgatgcggc
     2281 cgtgtcggtg ctgtcggtgc agcagggcga actgctcttc gcgaaccggt cctaccggct
     2341 gtggttcggc gcggatgcgc tgggccacgc gctgctggcc ggcgacgaca tcgatgccat
     2401 gcccgccgag cccgaagact cggtcgagag catggggggc ctgccgaccc aggagctgac
     2461 agagatcggc tcggatccgc gcgaggtgct gatcgaacca ctgaacaagt ggttcgacgt
     2521 gcgagtgagg tacctgcaat ggaccgacgg acgcctggca cagatgctta tcgcgacgga
     2581 cgtgacctcg cgtcggaacg ccgaaaccct ggccgccagc caatccgaga aggcccaggt
     2641 gaccagccgg ctggtgacga tgggcgaaat ggcgtcgtcg gtggctcacg agctcaacca
     2701 accgctgacc gcgatcgcga actactgcaa cggcatggtc tctcgggtga agaacgacgc
     2761 gatcgaaaag gaagacctga tcgcggcact ggaaaagacc tccaagcagg cacagcgggc
     2821 cggccagatc atccaccgca ttcgggcctt cgtgaaacgc agtgaaccgc agcggcagga
     2881 agctcaagcc cagaccatca tcgatgacgc cgtcgatctc gccagcatcg aactgcgccg
     2941 gcgcaaggta tcgatccaga cctacgtcgc acagcgtctg ccaccgctgc tggtcgatcc
     3001 gatcctgatc gagcaggtgg tcctgaacct gctcaagaac gcggcggaag cgatcgattc
     3061 ggccgatctt cccgctgcgc ggcgcaggat cgaactgcgc gtggtgccca aacgcaacgc
     3121 cgaggaaggc catgtgatcg agttcagcgt gaccgacatg gggccgggaa tccgtcccga
     3181 ggtcctggcg cgcctgtacg aggcgttttt ctccaccaag gcagaaggat tgggcatcgg
     3241 cctgagcctg tgccggtcca tcatcgagtc gcaccgtggc cgtatccgcg cggagaacct
     3301 ctacaatgcc gggtcggttt cgggctgctg ctttgctttc acgctgcccg tcgagagcag
     3361 ccgaatcgaa gcccttccct cacccaaccc ggtcgtgacg tcatgagctt gattccgaaa
     3421 aaaggcaccg tttacgtggt cgatgacgac gaagcggtgc gtgactctct acagtggctg
     3481 ctggaaggca aggattaccg ggtcaagtgc tttgactcgt ccgagtcgtt cctgtcgcgc
     3541 ttcgacccgc gcgaagtcgc ctgcctgatc gccgacatcc gcatggacgg aatgagcggc
     3601 ctcgaattgc aggaccgcct gatcgagcgc aagagcccgc ttcccatcgt gttcatcacc
     3661 ggacacggag acgtgccgat ggccgtcacg acgatgaaga agggggcgat ggatttcatc
     3721 gagaagccct tcaaggaaga agagctcgtg ggtcttgtcg agcggatgct ggacgaagcg
     3781 cgttccgcgt tctcgcagca ccaggaagcg gccagccgcg acgctctgct ctcgcgactc
     3841 accacccgtg aaggtcaggt actcgagcgc atcgtcgccg ggcgcttgaa caagcagatc
     3901 gccgacgatc tgggcatcag catcaagacg gtggaagcgc atcgcgccaa catcatggag
     3961 aaattgaatg cgaacaccgt cgcagatctg ctgaagatcg ccctgggcgc gcagggcaag
     4021 tcctgagacc ccgtcactgc ggatcggatc ggcagtgaca tcgacgaagg ccgcctcgca
     4081 gcggcctttc gcttttttga agacgaccag tgtgcggtat ttcgcggggc cgcttcggcc
     4141 tctggccctg atcccgcact cgcggggtga tttcggcttt gtgatttggc cttcaggccc
     4201 cagattccag ttccggactt cctgaccgcc caggtccgct cacttgacca acatgaccaa
     4261 tccgctgcta cgcgccgacg ccctgcccgc cttcgacgag attcgtcccg agcatgtcca
     4321 gcccgcgatc gacacgttgc tggccacgac ggacgccgcc ctggaacgcg cagtcggtcc
     4381 cgacacgcct gcggaatacg acgcggtgtc gtccgtcctc gacaccgccg ccgagcatct
     4441 gcgctttgtc tggggagcca tcgggcacct caatgcagtg gcggacacgc ctgccctgcg
     4501 tgcggcctac aacgacgcgt tgcccaaggt cacggagcag tcgagccggc acgccgctga
     4561 tgagcggctc tacgccaagt acaaggcgat cgcgaagaac gcagcctgcg tcggttctgc
     4621 gagcagcctg acgcccgccc gcaagcaggc gctgagcaac gcgatgcgcg atttcgtgct
     4681 ggcgggtgcc gaattgcagg gcgaggccaa gcagcgcttc cagcgccttc aggaactgca
     4741 ggcggacctg tcgcagaagt tctcggagca cgtgctcgac gcaaccgacg gctacgcctg
     4801 ttacgccagc accgaggagc tcgctggcgt tcccgccgac gcccgcgagg cctcgagggc
     4861 cgctgccgcg gccgaaggca aggcgggtca caaggtcacg ttgcacttcc ccagctattt
     4921 cccggtcatg cagtacggcg agaaccgcgc gctgcgcgaa cggctgtaca ccgccaatgc
     4981 cactcgggcc agcgagatgg gcccgccgga tcaggacaac agcgaggtca tgcgcgaact
     5041 gatccggctc cgtcaggagg aggccaggct gctcggctac ccgagctacg ccgatgtatc
     5101 gctggtgccc aagatggcag cgtcgccgcg ccaggtgatg gacttcctgc gagatctggc
     5161 aggccgcgcc cgcccgcacg cacagcgtga tctggccgag ttgcgcgagt tcgcacgcac
     5221 cgagctcggc atcgacgatt tgcaggcctg ggatgtgccc ttcgcgagcg agaagctgaa
     5281 ggaagcccgg tatgcattca gcgaccagga ggtgaagcag tacttcaccg agccgaaggt
     5341 gcttgaaggc ctgttccgga tcatcgagac attgttcgag gtggcgatcc gcccggacag
     5401 cgcaccggtg tggcacccga gcgtgcgctt cttccgcatc gagcggcctg ccgaaccggg
     5461 gtcggatggg ccagccgagt tggtcggcca gttctacctc gacccctacg cccgtcccgg
     5521 caagcgacct ggagcctgga tggacgaggt gcgcggccgg tgggcgcgtc ccgaggggcg
     5581 gctgcaaacg ccggtcgcgc aactggtctg caacttcacg ccgccgctgc ccggcaaggc
     5641 cgcactgctc agccacgacg acgtcaccac gctgttccac gaattcggcc acggtctgca
     5701 ccacatgctc acgcaggtcg acgacatcgg cgtggccggc atttcgggcg tcgaatggga
     5761 tgcggtcgaa ctgcccagcc agttcatgga gaacttctgc tgggagtggg atgtcgtcaa
     5821 gcggctgacc gcgcacgccg acaccggtga gcctctgccg cgtgaactgt tcgaccgcat
     5881 gctggctgcc aagaatttcc agagcggcct gcagacgttg cgtcaggtgg agttctcgct
     5941 gttcgacatg cgcctgcacg ccgaaccggg cagcgaggat cgcatcgagg ccctgcttga
     6001 agaggtgcgc gacgaggtgt cggtattcaa gccgcccgcg ttcaaccgct tccagcacag
     6061 cttttcgcac atcttcgctg gcggatactc cgccggttac tacagttaca aatgggccga
     6121 agtgctttcg gcggacgcct gggccgcttt cgaagaaact tcgcagactg gcggtgtgct
     6181 caatgtcgag acgggtcgcc ggtaccggca gaccgttctc gaagccggag gcagccgtcc
     6241 agcgatggag agcttcaagg ccttccgtgg tcgctcgggc tcaggacatg ccctggtgac
     6301 gcagc
//
LOCUS       DAOORV010000185         6251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15217_length_6251_cov_12.017915, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000185 DAOORV010000000
VERSION     DAOORV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15217_length_6251_cov_12.017915"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..1403
                     /locus_tag="PKV56_16460"
     CDS             492..1403
                     /locus_tag="PKV56_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69453.1"
                     /translation="MGRLPEESLSLLISVVEGGRSYREVASDLGVASSTVERRIKGLL
                     RRLQQAGALCPIRVDWLDSLVAIRSEREIIMAAARAHDERAPPPACNFVLDAEEVAAG
                     ASRLRACSRSANRDIALIYTLLCTGLKTMELALLEVRDYLDEHGEVKRVSTMRAEAAA
                     SGIERPLFFNSTRAVASIDIYLEERIRRRLGIGEPGRFRGLDPNSRLFLTEAGRPFLI
                     TRRSRTDSRLTSKYLQATLRLAFARAGWVGMTAQQARRQVALSLDRQGADRAQLQELL
                     GLRSPRQVRRLLRQPPMPLEVISHEIV"
     gene            1412..2710
                     /locus_tag="PKV56_16465"
     CDS             1412..2710
                     /locus_tag="PKV56_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HOX69454.1"
                     /translation="MSPDAQEVAMDVAKYFIRGVTLLATDPGLQEALSKVYETSERPR
                     CMCVRGGVEMYIAKHGEYVIKRMPGTGDMHHPTCQSFEPEPGISGLGELVGEAIVEHN
                     ADHVEIRTDFPFSRVSGKALPRGEPNGEPPAVNAPRKRMSLRAVLHFLYHRAGLNRWY
                     PAMEGKRSQGVIKKYLELAAAGVTLKGETLDKRLYVPEPFRVADKEEIGERRRRKLAM
                     LLSPGDDVAYKMAIVIGQFNGAEQSAYGRKLLIKHMPDVPLYMENKAWERAERAYAAT
                     LQARDADLERKPMVVMAALIYAKREHLYQVDSLSMTLVSDQWIPLEGLHELPLVEELQ
                     RQGRAFLKPLRFDAKSGACFPNALLLDTEAGPFPLHVMSAFLDPKEQSAKEKAVLAAG
                     GDAWVWRTDQALPALPPRAGVRTPSAGSDPTRGQRIPEVG"
     gene            2876..5041
                     /gene="dinG"
                     /locus_tag="PKV56_16470"
     CDS             2876..5041
                     /gene="dinG"
                     /locus_tag="PKV56_16470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HOX69455.1"
                     /translation="MVRDEEKQLIRQCVEALREGIPGFRSRRPQMEMIAAVANTLSRC
                     RAEDEQAGNGDHLAIVEAGTGTGKSFGALVPALVMAKSRNKRLVVSSSTVALQHQYAE
                     KDAPSLQRLLPIPFTFAVAKGRRRYACTVKLQAEGKLAGQAGLDLVGEGAPEGTVDAP
                     RRVAAMRELLVTFDTGRWSGDRDELPLPVVDEVWERITTDRQGCAGSKCPEFARCPFQ
                     AARQRVKEADLVIANHDLVLSAIDMDGASVLPSPQETIYVFDEAHSLAAKAVEHFSAR
                     HALRGAMEWLEGACNAVRDAVLGLRLDEQLIRDSRASAERMERALRDLHSRIHGTNGF
                     EEKRARRFKSGPLPDWCKSAGGQVLAAGEELQKTFASLREHVLEKAPSVGALATQVLS
                     VLGFYVVRLDNLVDTWQLMLTEQPEGSPPIARWIERHDEGGRPDDYQVCASPISGSDK
                     LRKLLWNRASGAVVMSATLTSCGTFDLFLRQSGLGYFDSPAFLRVESPFDFRNNARLV
                     VPAMRTEPTSAEQHTLEVSARLPDLVQTLGTLVLFTSARRMREVYAQLPEDLRRITLV
                     QGSMPKGEMLARHRAAVDRADRSVLFGLSSFAEGVDLPGEYCTHVVIAKLPFSVPDSP
                     LEEARREWVESRGGSPFIEITVPEAAVRLKQGLGRLLRTIHDRGRVTILDRRIVTKRW
                     GGLLMRGMPDFEVVIERGRASRPTAARAEDLKEPRWAGP"
     gene            5077..>6251
                     /locus_tag="PKV56_16475"
     CDS             5077..>6251
                     /locus_tag="PKV56_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69456.1"
                     /translation="MVAMAQSKANGQGGGLVPAAGGAVAIPTLLGRSAQRIPVGGRIR
                     AGIKVLTRRAAESGQAREIYDAGVAQGKGFDAIERELAAALPDLKNPLTPKNVPYFTV
                     RGEDFPNPELARQIMDLYAEDRGDGVQRLYRFPVVFPADAWQNVMPHELVTWTSSERR
                     FWSEYSEDGQTRYCKTYEQPPAPGAGRRVVRIFGGRKVTLRAENNGLCDPESCAEYQG
                     KKCNLSGRFIFFVPGIKSISAFELPTNSFYAMQAAIEKFQTIGFMRGGRISGFLDGQR
                     TPFFISKRLVEVSRIDEEGRPTRVAQWLIELEAPVDPTALLRHDEDLDALELRAADAA
                     NLLQGQGGGASAVQVVSEDGVIEVSGPPREVDRADVRGRAASGDGAPARHAPSARPAP
                     "
BASE COUNT         1083 a         1941 c         2214 g         1013 t
ORIGIN      
        1 tcggggtgtc gcccgagctc gggttgggga tgtgcgggga gaccccgcgg agaactgcct
       61 acaacccgtc gaagtcgcca ccgctgggcg gcaggcgctg ggttgtctgc tgagccgata
      121 gctgccggcg aaggatgtgg gtggcctgcg ggcgcaggca gattgaagtg cgggtcacga
      181 ccgctaacgc cggccaggcc ggaacgctgg tgacagcggc aatggcaggt atgccgatag
      241 cgcactcagt atgaatcaac gatgcgacgc cggaaagggg gtcgcatggc gcgtgcccat
      301 ctgtcactcg gtcgccggcg cccagcggcg gctcgctggg cgagccagcc cgtgcgacgg
      361 ccgaattccg gggggacttc gagccgcctt tccggttgaa ggcggccggc aagttcggga
      421 cgatcggcag cacggcaact caaccgatcg tggcgttgtc gttcgttccg tcaaccggtc
      481 gagatcagtc catggggcgc ctacccgaag aatcactgag cctgctcatc agcgtggtcg
      541 aaggtggccg gagctacagg gaagtggcgt ccgatctggg cgttgcgagc tcgacggtcg
      601 agcgtcggat caagggcctg ctgcgtcggc ttcagcaggc tggtgcgctg tgtccgatcc
      661 gggttgactg gttggacagc ctggtggcga tacgcagcga gagggagatc atcatggccg
      721 cggctcgcgc acacgatgaa cgtgcaccgc cgccggcttg caacttcgtc ttggatgccg
      781 aagaagtcgc cgccggtgcc agccgcctgc gcgcatgcag ccgcagcgca aaccgagaca
      841 tcgcgttgat ctacacgctg ctgtgcacgg gtctcaagac catggagctc gcgcttctag
      901 aggtgcgcga ctacctggac gaacacggag aagtcaagcg agtctcaacc atgcgcgcgg
      961 aggcggctgc aagcggcatc gagcggccgc tgttcttcaa ctcgacgcgt gcagtggcca
     1021 gcatcgacat ctacctggaa gagcgcatcc gtcggcgtct gggtatcggc gagccgggtc
     1081 gcttccgggg gctcgatccg aacagccgcc tgttcctgac cgaagcgggc aggccgttcc
     1141 tgatcacacg tcgctccagg accgactcgc ggctcacctc caagtacctg caggcgacgc
     1201 tgcggcttgc gttcgcgcgt gccggatggg tgggcatgac ggcacagcag gcccgtaggc
     1261 aagtggccct ttcgctggat cggcaggggg ccgacagagc ccagcttcag gaactgctgg
     1321 gattgcggtc tccacgccaa gtgcggcggc tgctcagaca accacccatg ccgctcgagg
     1381 tcatctctca cgagatcgtg taggccagat gatgtcccca gacgcccagg aggtcgcaat
     1441 ggacgtggcg aagtacttca tcaggggcgt cacgctgctg gcgaccgacc ccgggctgca
     1501 agaggcgctg tcgaaggtgt acgagacatc ggaacgtccg cgctgcatgt gtgtgcgtgg
     1561 cggcgtggag atgtacatcg ccaagcatgg cgagtacgtc atcaagcgca tgcccggcac
     1621 cggagacatg caccatccaa cgtgccagtc gttcgagccc gagccgggaa tatcgggcct
     1681 gggtgaactc gtcggcgagg ccatcgtgga gcacaacgcc gaccatgtgg aaatccggac
     1741 ggacttcccg ttctcccggg tatccggcaa ggcgttgccg agaggcgaac ccaacggcga
     1801 gccgcctgcc gtgaacgcgc ctcgcaagcg catgagtctg cgggcggtac tgcactttct
     1861 ctaccatcga gccggtctca atcgttggta tccggcgatg gagggcaagc gctcccaggg
     1921 cgtgatcaag aagtacctcg agcttgcggc agccggcgtg acactcaagg gcgagacctt
     1981 ggacaagcgc ctgtacgtgc cggagccctt ccgggtcgcc gacaaggaag agatcggtga
     2041 gcgaaggcgg cgcaagctgg ccatgctgct ttcgcctggt gacgacgtgg cctacaagat
     2101 ggcgatcgtc atcgggcagt tcaacggtgc tgagcaaagc gcctacgggc gcaagctgtt
     2161 gatcaaacac atgccggacg tgccgctgta catggagaac aaggcctggg aacgcgccga
     2221 gcgcgcctat gcggccaccc tgcaggcccg cgacgctgat cttgagcgca agccgatggt
     2281 cgtgatggct gcgctgatct acgccaagcg cgagcacctc tatcaggtgg actcgctgtc
     2341 gatgaccctg gtgtcagacc agtggatacc gctcgaaggc ctgcatgaat tgccgctggt
     2401 cgaggagctg caacggcagg gccgcgcctt cttgaagccg ctgcgcttcg atgccaagag
     2461 tggtgcctgt ttcccgaacg cactcctgct tgacactgag gctgggccgt tcccgttgca
     2521 cgtgatgagc gccttccttg acccgaagga gcagagcgcg aaggagaagg cggttctggc
     2581 cgcgggcggc gacgcatggg tgtggcggac cgaccaggcg ctcccggcac tgcccccgcg
     2641 tgcgggcgtt cggacgccga gtgctgggtc cgacccgacg cgagggcagc gcatccctga
     2701 agtcggatag ccgagacgag ctccggggtt gattgcccta gaggcgggtc ccacctgttc
     2761 gtcaggccgg cgtatcgcat gcccacaacg cgactcacct gcggatgctg agcggccagc
     2821 agtagcttgc tgcgaacatg cttgagtctg aaggtgcggc ccgtaggctg gccgcatggt
     2881 ccgcgatgaa gagaagcaac tgatccgcca gtgcgtcgag gcgctgcgcg aaggcattcc
     2941 gggcttcagg tcgcgccggc cgcagatgga gatgatcgcg gccgtggcca acacgttgtc
     3001 gcggtgccgc gccgaggacg agcaggcggg caacggcgac cacctcgcga tcgtcgaggc
     3061 cggcaccggt acaggcaagt cgtttggtgc gctggtgccg gcgctggtga tggcgaagtc
     3121 ccggaacaag cgcctggtgg tgagcagttc gacggtggcg cttcagcacc agtacgcgga
     3181 gaaggacgcg cccagcttgc agcgcttgct cccgatccct ttcacctttg ccgtcgccaa
     3241 gggccgacgc cgctacgcct gtactgtcaa gctgcaggcc gaaggcaagc tcgcgggaca
     3301 ggcaggcctc gacctggtgg gggagggagc tccggagggt accgtcgacg caccccggcg
     3361 ggtggccgcg atgcgggagt tgctggtcac cttcgacacc ggtcgttgga gcggggatcg
     3421 cgacgagctg ccgctgcccg tggtcgatga ggtttgggag cgaatcacca cggatcgcca
     3481 ggggtgcgcc ggctccaagt gtccggagtt cgcacgctgt ccgttccagg ccgcgcggca
     3541 gagggtcaag gaggcagacc tcgtgatcgc caaccacgat ctggtgttgt cggcgatcga
     3601 catggacgga gcaagcgttt tgccgtcgcc ccaggaaacc atctatgtgt tcgacgaggc
     3661 ccacagcctg gccgccaagg ctgtagagca cttctcggcg agacacgccc tgagaggggc
     3721 gatggaatgg ctggaggggg cgtgcaacgc ggtgcgtgac gccgtactgg gtttgaggct
     3781 ggacgaacag ctgattcgcg acagtcgggc cagcgctgag cgcatggagc gggcgctgcg
     3841 cgatcttcat tcccgaattc acggcaccaa cggattcgag gaaaagcggg cgcgccgctt
     3901 caagtccggg ccgttgccgg actggtgcaa gtcggccggt gggcaggtcc tggctgctgg
     3961 cgaagagttg cagaagacct ttgcttcgct cagggagcat gttctggaga aggcgcccag
     4021 cgtgggcgcg ctggccacgc aggtgctgtc ggtgttgggc ttctacgtgg tccggctgga
     4081 caacctggtg gacacctggc agctgatgct gaccgagcaa cccgaaggct cgccgccaat
     4141 cgcgcgctgg atcgagcggc atgacgaagg cggcaggccg gatgactacc aggtctgcgc
     4201 gtcgccgatc agcggcagcg acaagctgcg caagctgctg tggaatcgcg ccagcggtgc
     4261 cgtggtcatg tccgccaccc tcacgtcgtg cggcaccttc gacctgttcc tgcgccagtc
     4321 gggtcttggc tacttcgaca gccccgcatt cctgcgggtc gagtccccct tcgacttccg
     4381 caacaacgcg cggctcgtgg tgccggcaat gcgcacggag ccgacctccg cggagcagca
     4441 cacgctggag gtcagtgccc ggctgccgga cctcgtgcag acgctgggca cgctggtgct
     4501 cttcacgtct gcacggcgaa tgcgggaggt ctacgcccag ttgcctgagg acctgaggcg
     4561 gatcaccttg gtccagggct caatgcccaa gggcgagatg ctcgcgcggc accgtgccgc
     4621 cgtcgaccga gccgatcggt cagtgctgtt cgggctgagc tcgttcgcgg aaggcgttga
     4681 cttgccgggg gagtactgca cccacgtggt gatcgccaag ctcccgttct cggtgccgga
     4741 ctcaccgctc gaagaggcgc gacgcgagtg ggtcgagtcg cgtggtggat cgccattcat
     4801 cgagatcact gtgccggagg ccgcggtgcg gctgaagcaa ggcctgggga gactgctgcg
     4861 aacgatccat gaccgaggcc gagtgaccat cctggacagg cggatcgtga ccaagcgctg
     4921 gggtgggctc ctcatgcgcg gcatgcccga tttcgaggtc gtgatcgagc gtggtcgcgc
     4981 aagccgccca acggcggccc gtgctgagga cttgaaagag ccgaggtggg cgggaccatg
     5041 aatctccaca ttcaccaact tgcgaaggag agatgaatgg tcgcaatggc acagagcaag
     5101 gcgaacgggc agggcggtgg cctggtcccg gccgcgggcg gcgcggtggc gatccccacg
     5161 ctgctgggcc gctcggcgca gcgcatcccg gtgggcggcc gcatccgggc cggcatcaag
     5221 gtgctgaccc ggcgagcggc cgagtccggc caggcgcgcg agatctacga cgccggcgtg
     5281 gcgcagggca aggggttcga cgcgatcgag cgggaactcg ccgcggcgct gcccgacctc
     5341 aagaacccgc tcacgccgaa gaacgtcccg tacttcaccg tccgaggcga ggacttcccg
     5401 aaccccgaac tcgcgcggca gatcatggac ctgtatgccg aggaccgcgg tgacggtgtc
     5461 cagcgcctgt atcgcttccc agtggtgttc ccggccgatg cctggcagaa cgtcatgccg
     5521 catgagctgg tgacctggac ttccagtgaa cggcgcttct ggtccgagta ctccgaggac
     5581 gggcagacgc ggtactgcaa gacctacgag cagccgccgg cgccgggcgc aggccgccgc
     5641 gtcgtgcgca tcttcggcgg gcgcaaggtg acgctgcgcg cggagaacaa cgggctgtgc
     5701 gatcccgaga gctgcgccga gtaccagggc aagaagtgca acctgtcggg ccggttcatc
     5761 ttcttcgttc ccggcatcaa gtcgatcagt gccttcgaac tgccaacgaa cagcttctac
     5821 gccatgcagg cggcgatcga gaagttccag accatcggct tcatgcgcgg cggccgcatc
     5881 tccgggttcc tcgatggcca gcgcacgccg ttcttcatca gcaagcgcct ggtggaggtc
     5941 tcgcggatcg atgaggaagg ccgtccgacc cgtgtcgcgc agtggttgat cgaactcgag
     6001 gcgccggtgg acccgaccgc gctgctgcgc catgacgagg atctcgacgc gctcgagctt
     6061 cgtgctgcgg atgcggccaa cctgctccag gggcaaggtg gcggggcgag cgcggtccag
     6121 gtggtcagcg aagacggcgt gatcgaggtc tcgggcccac cccgagaggt tgatcgagcc
     6181 gacgtgcgcg gacgggcagc ttcgggagac ggcgcgccgg cacggcacgc accatcggcg
     6241 cgacccgcgc c
//
LOCUS       DAOORV010000186         6242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15240_length_6242_cov_9.340876, whole genome shotgun sequence.
ACCESSION   DAOORV010000186 DAOORV010000000
VERSION     DAOORV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15240_length_6242_cov_9.340876"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKV56_16480"
     CDS             complement(<1..228)
                     /locus_tag="PKV56_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HOX69457.1"
                     /translation="MTLRSPRFLIALACAATLSAPAFSQARQFLVYDGKSYGPDQLDI
                     AAVSPVAVAAPKRLPNGDFAVPRAADGHFYIA"
     gene            complement(225..956)
                     /locus_tag="PKV56_16485"
     CDS             complement(225..956)
                     /locus_tag="PKV56_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69458.1"
                     /translation="MRPLLSFAIALACMPALATDQLPAPAPAPVAVPQPVAPVDVAVS
                     GAFDVAADLGRTVYVEALASSHLTGVLRDALARRGLSLVASRDQATVVYELDGAFQAL
                     RPATQRRADIRLGDYAENPAPLATKSGRGPSVMLSLNPLAILIGTIASNVGNATGAQD
                     SVNAATAGDPDGKCLSKCDEWRYKQRNVVTLTRSGLDGTATCSALATVEDAALRPAEL
                     IQTSLDALASAIGLPDALAGAVARP"
     gene            complement(961..2034)
                     /locus_tag="PKV56_16490"
     CDS             complement(961..2034)
                     /locus_tag="PKV56_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX69459.1"
                     /translation="MTTSPALSATEAAVLRALLGGPVSGAQRLAEAAAGCRLSADLAR
                     VHRHVLAIRALVVRRYVRSRPDHGRARLEITRAGRRALRPASPRLALWFLTPVMALAA
                     SACTTTPPAAPSRMPLMGYPTMTGLAQVRDAKTGEAYFVPCNPCASPTVKTLAGADDA
                     RPAAAIPRRQALEGVVDRRAVLESLAAPPAPAFEPTPAPAGVLSPPASTAAVAAATGF
                     TASAATVIPQSLVAAAPVPDKLAVLFASASAELDAEALRALAPLLRAAAQGGHVTIRG
                     GADASGSTAANRTLAAKRAAAVRAAFLAAGVPAARLKVSVCATCYVAPNATPDGRRAN
                     RRADVELAMPSQAETGDAGAHKG"
     gene            complement(2043..2318)
                     /locus_tag="PKV56_16495"
     CDS             complement(2043..2318)
                     /locus_tag="PKV56_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOX69460.1"
                     /translation="MNRQDLIDAIAADTESSKAAVGRFLDSFINQVQGTVASGDAVKL
                     SGFGTFEKANVAARAGRNPKTGETISIPPTVRPRFTPGATFKALVKG"
     gene            complement(2482..5511)
                     /locus_tag="PKV56_16500"
     CDS             complement(2482..5511)
                     /locus_tag="PKV56_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOX69461.1"
                     /translation="MTTKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTETELALA
                     SSRPGLHAQVYCILQIGYFKAKHAFFRFDWSEVEHDCAFVLSRYFHGESFEHKPISKH
                     EHYTQREWIADLFGYRPWAAEFLAQLAQQAAQTVRRDVMPGFIAAELIVWLNEHKIIR
                     PGYTTLQELVSEALSAERRRLAGLLSEVLDESAKAALGRLLVRDDTLSQLAALKQDAK
                     DFGWRQMAREREKRATLEPLHRIAKALLPKLGVSQQNLLYYASLANFYTVHDLRNLKA
                     DQTYLYLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFVAEQVRRQQDTPQ
                     VGRLLSLYIDDSVPDPTPFGDVRQRAYKIMPRDTLQTTAQRMSVKPVSKLALHWQAVD
                     GLAERIRRHLRPLYVALDLAGTDPGSPWLVALAWAKDVFAKQQRLSQRPLAECPAATL
                     PKRLRPYLLTFDADGKPTDLHADRYEFWLYRQVRKRFQSGELYLDDSLQHRHFSDELV
                     SLDEKAAVLAQIDIPFLRQPLDAQLDALATELRAQWLAFNRELKQGKLTHLEYDKDTQ
                     KLTWRKPKGENQKAREKAFYEQLPFCDVADVFRFVNGQCQFLSALTPLQPRYAKKVAD
                     ADSLMAVIIAQAMNHGNQVMARTSDIPYHVLESAYQQYLRHATLHAANDCISNAIAAL
                     PIFPYYSFDLDALYGAVDGQKFGVERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNG
                     YLIGAHDYEAHHVFDIWYRNTSDIVPTAITGDMHSVNKANFAILHWFGLRFEPRFTDL
                     GDQLKELYSADDPALYDQCLIRPAGRIDRDLIVSEKPNLDQIVATLGLKEMTQGTLIR
                     KLCTYTAPNPTRRAVFEFDKLIRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSTIA
                     QVGGKKELTGRTDIEIEISNQCARLIANAVIFYNSAILSRLLMKYEASGNAKAHALLT
                     QISPAAWRHILLNGHYTFQSDGKMIDLDALVAGLELG"
     gene            complement(5508..6101)
                     /locus_tag="PKV56_16505"
     CDS             complement(5508..6101)
                     /locus_tag="PKV56_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOX69462.1"
                     /translation="MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKD
                     DRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAIVTSLKKKQVAFRSLTENLDTTT
                     PSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALD
                     GGMSKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR"
BASE COUNT         1092 a         1927 c         2183 g         1040 t
ORIGIN      
        1 ggcgatgtag aagtggccat cggccgcacg cggaacggcg aaatcgccat tgggcaggcg
       61 cttgggcgcc gcgacggcga ccggggaaac ggcggcaatg tcgagctggt caggcccata
      121 gctcttgccg tcatacacca gaaactggcg cgcctggctg aaggcaggcg ccgacagggt
      181 ggcagcacaa gctagggcga tcaggaagcg tggcgagcga agcgtcacgg ccgggcgacg
      241 gcgccggcca aggcgtcggg caagccgata gcgctggcca gcgcatcgag cgaggtctgg
      301 atcagctcgg ccggccgcaa ggccgcgtcc tcgaccgtgg cgagtgcgga gcacgtcgcc
      361 gtaccgtcca ggccggagcg cgtcagggta acgacgttgc gctgcttgta ccgccattcg
      421 tcgcacttgg acaggcattt gccgtcagga tcgccggccg tcgcggcgtt gaccgaatcc
      481 tgcgcgcccg tggcattgcc gacgttcgag gcgatggtgc cgatcaagat ggccagcggg
      541 ttgagcgaca gcatcacaga ggggccgcgc ccgcttttgg tagccagcgg cgccggattt
      601 tcggcgtagt cgcccaagcg gatgtcggcc cggcgctgag tcgccggccg cagcgcctga
      661 aaagcgccgt ccagctcgta gacaaccgtc gcttggtcgc gcgaagcgac caaagacaaa
      721 ccccgccgcg caagcgcatc gcgcagcacg cccgtgaggt gcgagctggc cagggcctcg
      781 acatagacag tgcggccgag gtcggccgcc acgtcaaagg cgccggacac ggcaacatcg
      841 acaggcgcca caggttgagg cacggcaaca ggtgcgggcg cgggagccgg aagctgatcg
      901 gtggccagcg ccggcatgca agcaagcgcg atagcgaacg aaagtagtgg tctcacgaca
      961 tcacccctta tgcgcgccgg catcgccggt ctcagcctgg gacggcatgg ccagctctac
     1021 gtcagcgcgg cggttggcgc gccggccgtc cggtgtggca ttcggcgcga cgtagcaggt
     1081 cgcgcaaacc gataccttca ggcgagccgc cggcacgccg gccgccagga aggcggcgcg
     1141 aacggccgcc gcgcgtttgg cggcaagcgt gcgattggcg gccgtcgaac cagaagcatc
     1201 ggcaccgccg cgaatggtga cgtggccacc ctgggccgcc gcgcggagca gcggtgccag
     1261 ggcgcgcagc gcctcggcgt ccagctcggc tgaagcggag gcgaacagca cggccagctt
     1321 gtccgggacg ggggccgccg ccacaagaga ttgcgggatc acggtggcgg cggaagcggt
     1381 gaagccagtc gcggcggcca cggcggcggt tgaggcaggc ggcgaaagca cgccggcagg
     1441 ggcaggcgtc ggctcgaaag cgggcgcagg aggggccgcc agactctcca ggacggcgcg
     1501 acgatcaacc acgccctcca gggcctgccg gcgggggatt gccgcagcgg ggcgcgcgtc
     1561 atccgcgccg gccagggtct tgaccgtagg ggacgcgcag ggattgcagg gaacgaaata
     1621 ggcttcgccg gtcttggcgt cacgcacctg ggccaagcct gtcatggtgg ggtagcccat
     1681 cagcggcatg cgcgagggcg cggccggcgg ggtggtggtg caggcgctcg cggccaaagc
     1741 catcactgga gtcaagaacc acaaggcgag gcgaggcgac gccggacgca gcgcccggcg
     1801 gccggcgcgc gtgatttcca aacgggcgcg gccgtggtcc gggcgcgagc ggacatagcg
     1861 ccgcaccacc aaggcccgaa tggccagaac atggcgatgc acgcgcgcaa ggtcggccga
     1921 cagacggcag ccggcggccg cttcggccag gcgctgcgcg cccgagaccg gcccgcccag
     1981 cagggcgcgc aagaccgccg cctcggttgc ggaaagggca ggggaggtcg tcatggtcgg
     2041 cctcagccct tgaccagtgc cttgaaggtg gcgccgggcg tgaagcgcgg tcgcaccgtg
     2101 ggcggaatgg aaatggtctc gccggtcttc gggttgcgcc cggcacgggc ggcgacattg
     2161 gctttttcga acgtaccgaa gccgctcaac ttcacggcgt caccgctggc cacggtgcct
     2221 tgcacctggt tgatgaaact gtcgaggaag cgcccaacgg cggctttcga ggattcggtg
     2281 tcggcggcaa tggcgtcgat cagatcttgt cggttcatgc agtaagtcct tgttgttggt
     2341 ggagtgggaa aagcggtttc gcgcgcgaaa gacagaaccg cacggcgatt gaggtaagca
     2401 gtagggcagg gcagtacggc atgggcgtca cgccggcagg gccggtagcg gggttctaag
     2461 ccagaaccgc cgaaatttcc gtcatcccag ctccagcccc gccacgagcg catccaggtc
     2521 gatcatcttg ccgtcgctct ggaaggtgta atgcccgttc agcaggatgt gccgccaggc
     2581 cgccggcgat atctgggtca ggagagcgtg cgccttggcg ttgccgctcg cctcgtactt
     2641 catcagcagc cgcgagagga tggccgagtt gtagaagatg accgcgttgg cgatcagcct
     2701 ggcgcactgg ttgctgatct caatttcgat gtcggtgcgc ccggtcaatt ccttcttgcc
     2761 gccgacctgg gcgatggttg agcgtagctg gtgataggac tcgatgcggt tctgtgagcg
     2821 gtgaacgttg cgctccagtt gcggatcgcg caggtagcgc agcgtgtaga tgctgcggat
     2881 gagcttgtcg aactcgaaca ccgcgcgccg cgtggggttc ggcgcggtgt aggtgcatag
     2941 cttgcggatc agcgtgccct gcgtcatctc cttcagtccg agcgtggcga caatctggtc
     3001 gaggttcggc ttctcgctga ctatgagatc gcggtcgatt ctcccggccg gccggatcag
     3061 gcactgatcg tacagcgccg gatcgtcggc actgtagagt tccttcaact gatcgccaag
     3121 gtcggtgaag cgcggctcga aacgcaggcc gaaccagtgc aggatagcga agttggcctt
     3181 gttgacgctg tgcatgtcgc cggtgatcgc ggtcggcacg atgtccgacg tgttgcgata
     3241 ccagatgtcg aacacgtgat gggcctcgta atcgtgcgcg ccgatcaggt agccgttgag
     3301 cggcacgtgg ttgcacagca gcgtgtaggc gaccacgccc ttgccgcgcc caaagtattt
     3361 gcgcgagtgg cgcgctttca cggtcggccg ctcgacgccg aatttctgac catcgacggc
     3421 accgtacagt gcatcgaggt cgaacgagta gtacgggaag atcggcagcg cggcgatggc
     3481 gttgctgatg cagtcgttgg ccgcgtgcag cgttgcgtgg cgcaggtact gttggtaggc
     3541 gctctccagc acgtggtaag ggatgtcgct ggtgcgtgcc atgacctggt tgccgtggtt
     3601 catcgcctgc gcgatgatga ccgccatcag gctgtcggcg tcggcgacct tcttcgcata
     3661 gcgcggctgc aaaggcgtca gcgccgacag gaactggcac tggccgttga cgaagcggaa
     3721 cacgtcggcc acgtcgcaga acggcagttg ctcgtagaac gccttctcgc gcgccttctg
     3781 gttctcgccc ttgggcttgc gccatgtcag cttctgcgtg tccttgtcgt attctaggtg
     3841 cgtcagcttg ccctgcttca gctcgcggtt gaaggccagc cactgagcgc gcagctcggt
     3901 cgcgagcgca tcgagctggg catcgagtgg ctgccgcagg aacgggatgt cgatctgcgc
     3961 cagcacggcg gccttctcat ccagcgaaac cagctcgtcg gaaaaatgcc ggtgctgcaa
     4021 gctgtcgtcg aggtagagtt cacccgactg gaagcgcttc ctgacctggc ggtacagcca
     4081 gaactcgtag cggtcggcat gcaggtccgt cggcttgcca tcggcatcga aggtcagcag
     4141 gtacggtcgc aagcgtttcg gcagcgtggc cgctggacat tcggcgagcg gccgttgcga
     4201 taggcgctgc tgtttggcga acacgtcctt ggcccaggcc agcgccacga gccacgggct
     4261 gcccggatca gtgccagcga ggtcgagcgc gacatacagc ggccgaagat ggcggcggat
     4321 gcgctcagcc aggccgtcca ccgcctgcca gtgcaaagcc agcttgctca ccggcttcac
     4381 gctcatgcgc tgcgcggtgg tttgcagcgt atcgcggggc atgattttgt aggcgcgctg
     4441 gcgcacatcg ccgaacggcg tgggatcggg cacgctgtcg tcgatgtaaa gcgacagcag
     4501 gcggccgacc tgcggtgtgt cttgctgacg gcgcacctgc tcggcgacaa aggattgctt
     4561 tgcgcccgca ctgctttcgt cctccaactg cttcatgtgg taggccatcg catcgaccag
     4621 gttgtcggaa agctgccggt agcgcaccca ggcatagcaa agcaggtaga ggtaggtctg
     4681 atcggccttc aggttgcgta gatcgtggac ggtgtagaag ttcgccaggc tggcgtagta
     4741 cagcagattc tgctgcgaga cgccgagctt gggcagcagc gccttggcga tccggtgcag
     4801 cggctccagc gtggcgcgct tttcgcgttc gcgggccatc tgacgccagc caaagtcctt
     4861 ggcgtcctgc ttgagcgccg ccaattgcga cagggtgtcg tcacgcacta gaagccgacc
     4921 cagcgcggcc ttggccgatt cgtccaacac ttccgacagc aggccagcca gccgccgacg
     4981 ctcggcggac agggcttcgc tcaccagctc ttgcagggtg gtatagccgg gccggatgat
     5041 cttgtgctcg tttagccaga cgatcagctc ggcggcgatg aaccccggca tcacgtcgcg
     5101 ccgcacggtc tgcgcggcct gctgcgcgag ctgcgccagg aactcggccg cccacggccg
     5161 gtagccgaac agatcggcaa tccactcgcg ctgggtgtag tgctcgtgct tggagattgg
     5221 cttgtgctcg aaggactcgc cgtggaagta gcggctcagc acgaaggcgc aatcgtgctc
     5281 gacctcactc cagtcgaagc ggaagaaggc atgcttggcc ttgaagtaac cgatctgcaa
     5341 gatgcaatag acctgggcat ggagaccagg ccggctgctg gcgagcgcca gttcggtttc
     5401 agtcaacgcc aagtattcca gccgctgggc gtcgtcgaag tccggcaggc cgtacagggc
     5461 ttcctgctcg gcgtccgaca ggacggtcaa tcgttcgctc ttggtcgtca tcgcgatgac
     5521 gctccacgag agcccgtcca accaacccgt gccagggtct cgatcagcgt ggttcgcttg
     5581 acgccgaagt tgcggcacac cgccgccttg gacatgccgc catcgagcgc agcgacgatg
     5641 gcctccagct tctcgccggt gatcgcctgc ggccggccgc cgatccggcc gcgtttgcgg
     5701 gcggcagcca gaccggcgac gacacgttcc tggatcaagg cgcgttcgta ctgcgcgagc
     5761 gcgccgaaca cctggaacag aaactcgccc gagggcgtcg tggtatccag gttctccgtc
     5821 agcgagcgga acgccacctg ctttttcttg agcgaggtca cgatggcgag caagtgcgac
     5881 aacgaacggc cgagccggtc gagcttccac acgaccaaca cgtcgccagg gcgaacgaat
     5941 tcgagcgccc gcgccaggcc cgcgcggtcg tccttcgcgc cggaagcatg atcctcgaac
     6001 agatgccgcg catcgacgcc gacggcgagc agcgcatcgc gctgcaagtt cgtgctctgg
     6061 cggtcggagt ccgacgacac gcgcatgtaa cctaccaaca taagcggaaa accattaaga
     6121 cgaggtttcc gtatggtagc gtctggcgac agagttttcc gcacaatttt gcggccactc
     6181 ggaggttggt gcagaggatc gttgaaggcc tgtcgaaaaa caaatgtttt ccgacaggcc
     6241 tt
//
LOCUS       DAOORV010000187         6152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15555_length_6152_cov_8.612432, whole genome shotgun sequence.
ACCESSION   DAOORV010000187 DAOORV010000000
VERSION     DAOORV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15555_length_6152_cov_8.612432"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..636)
                     /locus_tag="PKV56_16510"
     CDS             complement(376..636)
                     /locus_tag="PKV56_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOX69463.1"
                     /translation="MIAFEVKDMTCGHCVSTITKALKAADKDAKVQIDLATHRVQVEP
                     VSADAEELAEAIKDAGYTPVSVEAALSESAAPVRGSCCGHCK"
     gene            810..3260
                     /locus_tag="PKV56_16515"
     CDS             810..3260
                     /locus_tag="PKV56_16515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX69464.1"
                     /translation="MNTAALSEFKLQVTGMTCASCVTRVEKSLKAVPGVRQASVNLAT
                     EQALVSAEPAVTADTLAAAVRKSGYGVDLTETTLQVEGMTCASCVARVEKALLKVPGV
                     SGATVNLATEKASVQALSTVPVAALKAAIEKAGYAAKETTDATPQPALRLPAWWPVAV
                     GGVLTLPLVMPMLLQLFGIDWMLDGWLQLALATPVQFWLGWRFYRAGWKAVLAKAGNM
                     DLLVALGTTAAYGLSVYLLFKHADHGMPHLYFEASAAVITLVLLGKWLEQRAKRQTAD
                     AIRALNALRPTTARVRRGDVELDIPVDQVVVGDLVVVRPGDRVAVDGDIVEGRSHIDE
                     SLITGESLPVAKGVGDKVTGGAINAEGALTVRTTAIGAETTLARIIRMVESAQAAKAP
                     IQRIVDRVSAVFVPVVLVIALATFLGWFGFTGDWEHALINAVAVLVIACPCALGLATP
                     TAIMAGTGVAARHGILIKDAEALEVAHAVTVVAFDKTGTLTEGKPSLAAVVAAVGSTR
                     EEVLGLAGALQKSSSHPLALAVMDQVRHERLPVPEAHDAQALPGRGVQGVVRGHTLAL
                     GSTRLLREMGLEEGHLHTEAQRLEQHGRTVSWLVQSNAGQQRLLGLLAFGDKVKPAAA
                     EAVARLHQLGIRTVMLTGDNRGAADAVAKELGIDDVRAEVLPGDKAAIVKELRDAGEV
                     VAFVGDGLNDGPALAAASVSYAMAGGADVATETAGITLMRGDPRLVADSLDISRRTYS
                     KIKQGLFWAFGYNVLGIPLAAFGLLNPVIAGAAMAFSSVSVVLNALTLRRWHGVAEVR
                     TTKPTSRQPRPVAALGEQ"
     gene            3266..3739
                     /gene="cueR"
                     /locus_tag="PKV56_16520"
     CDS             3266..3739
                     /gene="cueR"
                     /locus_tag="PKV56_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HOX69465.1"
                     /translation="MNIGEAAKSSGVSAKMIRHYESVGLFPEAARTDSGYRQYTAKEV
                     HTLRFIRQSRDLGFSIEQIRELLGLWQDRKRPSRQVRALAQAHIEELDEKLKELHAMK
                     ATLEHLVHCCHGDDRPDCPIIDTLAHEGIAPAAAARHVGDRTAGLQPGRARRRAS"
     gene            complement(3768..4148)
                     /locus_tag="PKV56_16525"
     CDS             complement(3768..4148)
                     /locus_tag="PKV56_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69466.1"
                     /translation="MLSLRICGALAVATFCGGAFAASHIVDITWPPDGRFTHKAQIAA
                     GKFVEVCGKLAVGEGVRWNFTAAAPVDFNIHYHVGKEAVFPAKQTQVAVGRDTLSVTV
                     AQDYCWMWTNKGSAPVSLTVDLAR"
     gene            complement(4244..4615)
                     /locus_tag="PKV56_16530"
     CDS             complement(4244..4615)
                     /locus_tag="PKV56_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HOX69467.1"
                     /translation="MNQQRFFLRATGLALMVSAGALSPAWAASHQGGHSAHAEHAKPT
                     AQAATGDMTDGEVRKVDKEGGKLTLKHADIKSLDMPAMTMVFVVKDKAVLDKLKAGDK
                     IKFKAINDAGKFTVTELQMAP"
     gene            complement(4612..5103)
                     /locus_tag="PKV56_16535"
     CDS             complement(4612..5103)
                     /locus_tag="PKV56_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOX69468.1"
                     /translation="MSEVSRRRALASLLGAGGMLAIPAALRAQGQAVLVEVWKSPTCG
                     CCKDWITHLEANGFRVKVNDVGNTAARARLKVPMKLGSCHTAWVGRYAIEGHVPAADI
                     HRLLRQRPDAIGLAVPGMPLGSPGMDGPAYDNRQDPYDVLLIAHDGGTQVFNSYFKQK
                     ERS"
     gene            complement(5100..5645)
                     /locus_tag="PKV56_16540"
     CDS             complement(5100..5645)
                     /locus_tag="PKV56_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HOX69469.1"
                     /translation="MNEPKTISHARRTAVTRMALSAATAAMPLLATPARAHTDKPHAP
                     KKGPVVKEQKDWGIAGEAKSAKRSIEVGMADNMRFTPARIDVRQGETVKFVVRNTGEV
                     MHEFVIGTKSENGKHAEMMVKFPNMEHDEPYMAHVPPGKTGEIVWTFNRAGEFEFACL
                     IAGHYQGGMVGTIMVTPAGKA"
     gene            complement(5685..>6152)
                     /locus_tag="PKV56_16545"
     CDS             complement(5685..>6152)
                     /locus_tag="PKV56_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HOX69470.1"
                     /translation="HCHKSHHTMNAMGHDVPTMIGVDHRGVARQITKLIPDYMVMGER
                     GMKDMTEMEMPLPDNTAAMMTGTGPFGAVGMGGMFSVVKVRKDQKRGDYSDPGWYQHP
                     PGTVAYEFTGALPDPARFKADASPGPGSMPPVAKPTQDVEVKVRKPAGGHSGH"
BASE COUNT          975 a         2043 c         2033 g         1101 t
ORIGIN      
        1 gccacgaggc cgaccacgac gaccagcaga acaggtcccc agtagccgcc gtagctaccc
       61 atccagccag cgccacccat gccaccgttc atcatggttc cgccctgcgc agaagccagg
      121 ccgctcagcc ccaggggcaa ggccccggcc atcttcgtca gcacgttctt catggcaact
      181 cctcgcaaaa gcggtcggcc cctgctcttc gaggcagcgg tggggcggtt gaacttaggc
      241 ttgcagcgaa ctactgtctg tctgccagcg aacatggacc cgagatagtg ccgcgtcgtg
      301 ttcactccaa gcgcgagagc atgggtaaca cggtctcgcc acccacacac gcatcgacca
      361 ctgcttcagc ttccgctatt tgcagtgtcc acaacaggac ccacgcactg gcgcggcaga
      421 ttcgctcaga gccgcctcaa ccgacactgg cgtgtagcca gcgtccttga tggcctcggc
      481 cagttcctcg gcgtcggccg acacgggctc cacctgcacc cggtgcgtgg ccagatcaat
      541 ctgaaccttg gcgtccttgt cggcggcctt cagggccttg gtgatggtgc tgacgcagtg
      601 gccgcaggtc atgtccttga cttcgaaagc aatcattgtt ttgtccagtg ggttgttgat
      661 gtgtcgactg tgaactctcc cactgttgta aggtcaagga ctcgttccgg aaagccacaa
      721 tggcgtcagg cagatttatc gctgagtcta taccttgcca tcgttagaac cttgacgatg
      781 cccacatcga acaactcccg ggggcatcca tgaacaccgc cgcactttcc gaattcaagc
      841 tgcaggtcac gggcatgacg tgtgcctcct gcgtcacgcg tgtcgagaag tcgctcaagg
      901 cagtccccgg cgtcaggcag gccagcgtca acctggcgac agaacaggcc ttggtcagtg
      961 ccgagcccgc agtgactgca gacacgctgg ccgctgccgt gcgcaagtcc ggctacggcg
     1021 tcgatctgac cgagaccacg ctgcaggtcg aaggcatgac ctgcgcctcg tgcgtggccc
     1081 gcgtcgaaaa ggcgctgctc aaggtgcctg gagtttcggg cgccactgtc aatctcgcga
     1141 ccgagaaggc gagcgtccag gcgctgtcga cggtgccggt cgcggcgctg aaggccgcca
     1201 tcgagaaggc cggatacgcc gcgaaggaaa ctacggatgc gacgccccag ccggcgcttc
     1261 gtcttccggc ctggtggccc gtcgccgtgg gcggtgtgct gaccttgccc ctggtcatgc
     1321 cgatgctgct ccagcttttc ggcatcgact ggatgcttga cggctggttg cagctggccc
     1381 tagcgacccc cgtgcagttc tggctcggct ggcgcttcta ccgcgccggc tggaaggccg
     1441 tgcttgcgaa ggccggcaat atggatctgc tggtggcgct gggcacaacg gccgcctatg
     1501 ggctctcggt ctacctgctc ttcaagcatg ccgaccatgg catgccgcac ctgtacttcg
     1561 aggcctccgc agcggtcatc acgctggtgc tgcttggcaa gtggctcgag caacgcgcga
     1621 agcggcagac cgccgatgcc atccgggcgc tcaatgcgtt gcggccgacc accgcccgcg
     1681 tgcgccgcgg cgacgtcgag ctggacatcc cggtcgacca ggtcgtggtc ggcgacctgg
     1741 tcgtcgtgcg ccccggtgac cgcgtggccg tggacggtga catcgtcgaa ggccgcagcc
     1801 atatcgacga atcgctgatc accggtgaaa gcctgccagt ggccaagggc gtcggcgaca
     1861 aggtcaccgg tggcgccatc aacgccgagg gcgcgctgac cgtccgcacg acggccatcg
     1921 gcgcagagac gacgctggca cgcatcatcc gcatggtcga atcggcccag gccgcgaagg
     1981 cgccgatcca gcgcatcgtc gaccgcgtga gtgcggtgtt cgtgcccgtg gtgctggtca
     2041 ttgcgctggc caccttcctg ggatggttcg gtttcacggg tgactgggaa cacgcactca
     2101 tcaacgccgt agccgtgctc gtcatcgcct gcccttgtgc gctcgggctg gccacgccga
     2161 cggccatcat ggccggcaca ggcgtcgctg cgcgccacgg catcctcatc aaggatgccg
     2221 aagcgctgga ggtggcgcac gcggtcaccg tggtcgcgtt cgacaagacc ggcacgctca
     2281 ccgaaggcaa gccgtcgctg gccgcggtgg tggccgcagt gggctccacg cgggaagagg
     2341 tgctgggcct ggccggcgcg ctgcagaagt cgagcagcca tccgctggcc ctggcagtga
     2401 tggaccaggt ccgccatgag cgcctgccgg tgcccgaggc gcacgatgcc caggccctgc
     2461 ctgggcgcgg ggtgcagggc gtcgtgcgcg ggcatacgct ggccctgggc agcacgcgcc
     2521 tgctgcgcga gatgggtctc gaggaagggc atttgcacac cgaagcccag cgccttgagc
     2581 agcatggccg gacggtgtcg tggctggtgc agtccaatgc cggccagcaa cgactgctgg
     2641 gccttctggc ctttggcgac aaggtcaagc ctgctgctgc ggaggcggtg gcgcggctgc
     2701 accaactcgg catccggacc gtgatgctga caggcgacaa ccgcggcgcg gccgacgccg
     2761 tcgcgaagga actgggcatc gacgatgtgc gtgcggaagt gttgcccggc gacaaggcgg
     2821 ccatcgtcaa ggaactgcgt gacgccggag aagtggtggc cttcgttggc gacggtctga
     2881 acgacggacc ggcgctggcc gctgcctcgg tgtcgtacgc gatggcgggc ggcgccgatg
     2941 tggcgaccga gaccgccggc atcacgctga tgcgtggcga cccgcggctg gtggccgact
     3001 cgttggacat ctcgcgccgc acctactcga agatcaagca gggcctgttc tgggccttcg
     3061 gctacaacgt gctgggcatc ccgctggctg cgttcggact cctgaacccg gtcatcgccg
     3121 gcgccgcgat ggccttcagc agcgtcagcg tcgttctgaa tgcgctgacg ctgcgccgct
     3181 ggcacggtgt cgctgaggta cgcacgacca agccgacaag ccgccagcct cgacccgtgg
     3241 cggctttggg agagcagtga tgagcatgaa tatcggcgaa gccgcgaagt cctccggtgt
     3301 gtcggcgaag atgatccgcc actacgagag cgtcggcctc ttcccggaag cggcgcgaac
     3361 cgactccggc taccggcagt acaccgccaa ggaagtgcac acgctgcgct tcatccgcca
     3421 gtctcgcgat ctgggctttt ccatcgaaca gatccgcgag ttgctcggcc tgtggcagga
     3481 ccgcaagcgg ccgagccggc aggtgcgcgc gctggcgcag gcgcacatcg aggagctcga
     3541 cgagaaactc aaggagctgc acgccatgaa ggccacgctc gagcacctcg tgcactgctg
     3601 ccacggtgac gaccggccgg actgtcccat catcgacacg ctcgcgcacg agggcatcgc
     3661 gccagcggct gcggcgcgtc acgtgggcga caggaccgct ggactgcaac cggggcgggc
     3721 gcgtcgccgg gccagctaac tgacggattg gccgctgcga ttcgagatca gcgcgccagg
     3781 tcgaccgtca aacttacagg tgcgctgccc ttgttcgtcc acatccagca gtagtcctgc
     3841 gctacggtga cgctcagcgt gtcgcgacca acggcgacct gcgtctgctt ggcagggaac
     3901 acggcctcct tgccgacgtg atagtggata ttgaagtcca ctggcgccgc agccgtgaag
     3961 ttccagcgca caccctcccc caccgccagc ttgccgcaga cctcgacgaa cttccctgcc
     4021 gcgatctggg ccttgtgcgt gaagcggccg tcaggcggcc aagtgatgtc cacgatgtgg
     4081 ctggcggcaa acgcgccccc gcagaacgtg gccacggcga gagcgccgca gattcgtaga
     4141 ctcagcatgc tcatgatttc ttgtgcgacg aactgttgag gcgaccattt tcggcgcaac
     4201 aagggcgggc gcccgacccg agcggcggtc gggcctcctt ggttcacggc gccatctgca
     4261 gctccgtgac cgtgaactta cccgcgtcgt tgatggcctt gaacttgatc ttgtcgccgg
     4321 ccttcagctt gtccagcacc gccttgtcct tgacgacgaa gaccatcgtc atcgccggca
     4381 tgtccaggct cttgatatcc gcgtgtttga gggtcagctt gccgccctcc ttgtccacct
     4441 tgcgcacctc gccgtcggtc atgtcgccag tggcggcctg tgcggtcggc ttggcatgct
     4501 cagcatgcgc actgtggccg ccctggtggc ttgcagccca agccggcgac agggccccgg
     4561 ccgaaaccat aagcgccagg ccggtggcac ggaggaagaa tcgttgttgg ttcatgagcg
     4621 ttccttttgc ttgaagtagc tgttgaagac ttgggtgccg ccgtcatggg cgatgagcag
     4681 cacgtcgtag gggtcctgcc ggttgtcgta ggcaggaccg tccatacccg gggaaccgag
     4741 cggcatgcct ggcacggcga ggccgatcgc atcggggcgc tgcctgagca gccggtggat
     4801 gtcggccgcc ggcacatgcc cctctatcgc atagcggccc acccaggccg tgtggcacga
     4861 gccgagcttc atggggactt tcagccgagc ccgcgccgcg gtgttgccga catcgttgac
     4921 cttgacgcga aagccattgg cttcgaggtg cgtgatccag tccttgcagc agccgcaggt
     4981 cgggctcttc cagacctcca ccagcacagc ctggccctgc gcgcgcagag cggcggggat
     5041 ggcgagcatc ccgccagcgc cgagcagcga ggccagcgcg cgtcgcctgc tcacctcgct
     5101 catgccttgc ccgccggcgt gaccatgatc gtgcccacca tgccgccctg gtagtggccg
     5161 gcgatcaggc aggcgaactc gaactcgccg gcgcggttga aggtccagac gatctcgccg
     5221 gtcttgccgg gcgggacatg cgccatgtag ggctcgtcgt gttccatgtt cgggaacttc
     5281 accatcatct cggcatgctt gccgttctcg gatttcgtgc cgatgacgaa ctcgtgcatg
     5341 acctcgccgg tgttgcgaac cacgaacttc accgtctcgc cctgtcgcac atcgatgcgc
     5401 gcaggcgtga agcgcatgtt gtcggccatg ccgacctcga tgctgcgctt ggcgctcttg
     5461 gcttcgccgg cgatgcccca gtccttctgc tccttgacca ccgggccctt tttcggcgcg
     5521 tgcggcttgt cggtgtgagc ccgcgcgggc gtggccagca ggggcatcgc cgccgtagcg
     5581 gcggaaagcg ccatgcgcgt cacagcggtg cggcgggcgt gcgagatcgt cttcggttcg
     5641 ttcatgcggt actccttgaa tggtgagagt gagtcttgtt gcggtcagtg gcccgagtgg
     5701 ccgcccgcgg gcttgcgcac tttgacctcg acgtcctgcg tcggcttggc cactggcggc
     5761 atggagcccg gcccagggct ggcgtcggcc ttgaagcgcg ccgggtcggg caaggcgccc
     5821 gtgaactcgt aggccaccgt gcccggcggg tgctggtacc aacccgggtc gctgtagtcg
     5881 ccccgcttct ggtccttgcg caccttcaca acgctgaaca tgccgcccat gccgacagcg
     5941 ccgaacggcc cggtgcccgt catcatggca gcggtgttgt cgggcagcgg catctccatc
     6001 tcggtcatgt ccttcatgcc gcgttcgccc atcaccatgt agtcggggat gagcttggtg
     6061 atttgccgcg ccacgccgcg gtggtccaca ccgatcatgg tcggcacgtc gtggcccatc
     6121 gcgttcatcg tgtggtggct cttgtggcag tg
//
LOCUS       DAOORV010000188         6132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15609_length_6132_cov_6.327958, whole genome shotgun sequence.
ACCESSION   DAOORV010000188 DAOORV010000000
VERSION     DAOORV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15609_length_6132_cov_6.327958"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..560
                     /gene="rpsF"
                     /locus_tag="PKV56_16550"
     CDS             201..560
                     /gene="rpsF"
                     /locus_tag="PKV56_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOX69471.1"
                     /translation="MRHYEIVLLIHPDQSEQVPAMLERYKGVITAGGGKVHRVEDWGR
                     RQMAYMIQKLAKAHYLCINIECSKEVLAELETGFRFNDAVLRHLTVLKSKAETAPSVM
                     MKTSDREDRRAPQESAA"
     gene            598..879
                     /gene="priB"
                     /locus_tag="PKV56_16555"
     CDS             598..879
                     /gene="priB"
                     /locus_tag="PKV56_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HOX69472.1"
                     /translation="MNRLVLRAAVVERGALRYTPAGLPALDLSLRHESQVTQNGMPRQ
                     VSMEIKARAVGDITKALNCIELGSTHGFAGFLGSQRNGKGIVFHVTELD"
     gene            925..1215
                     /gene="rpsR"
                     /locus_tag="PKV56_16560"
     CDS             925..1215
                     /gene="rpsR"
                     /locus_tag="PKV56_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOX69473.1"
                     /translation="MPPPRGKGKFSKDRKPKRNQQSLLFRRKRFCRFTVTGVTEIDYK
                     DVETLRDFIGENGKITPARLTGTRAFFQRQLTTCIKRARFLAMLPYSDQHRV"
     gene            1229..1681
                     /gene="rplI"
                     /locus_tag="PKV56_16565"
     CDS             1229..1681
                     /gene="rplI"
                     /locus_tag="PKV56_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOX69474.1"
                     /translation="MQIILLEKVANLGDLGDVVKVKDGYARNFLIPSRSARRATPTAI
                     KEFEVKRAELEKAAADKLAAAQALGEKMSGRTVHITQKAGVDGRLFGSVTNNDIAEAL
                     TRIDFKVVKSQVRLPNGPLKTVGEHAVSVSPHTDVVVEVKVVVVGETA"
     gene            1815..3242
                     /gene="dnaB"
                     /locus_tag="PKV56_16570"
     CDS             1815..3242
                     /gene="dnaB"
                     /locus_tag="PKV56_16570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOX69475.1"
                     /translation="MSALLSSPQLTSQPPRGDDEVARLRMPPHSIEAEQSVLGGLLLD
                     NSAWDRAGDLLTDSDFYRFEHRAIYAAIGALVNATRPADVITVFEQLQSLGKAEECGG
                     LVYLNALAQSVPSAANLRRYAEIVRERAVLRKLVSASDEIATSAFNPQGRAVSEILDE
                     AEAKIFKIGEEGSRSRQGFQTMDSLVVQLLDRVTELHENGAEEVTGVRTGYFDLDRMT
                     AGFQPGDLIILAARPSMGKTALALNIAEHVAVQEGLPVVVFSMEMGAAQLALRLVGSL
                     GRIDQQHLRTGALKDDEWGRLTEAIEKLGSVSMYIDESPALTPSELRARARRQARQCG
                     KLGLIVVDYLQLMSGSGGANEENRATVIGEISRGLKSLAKELQCPVIALSQLNRSVES
                     RTDKRPMMSDLRESGAIEQDADVIMFIYRDEYYTKEACKEPGVAEIIIGKQRNGPTGT
                     VKLTFLKPLTKFDNLAPGSAGSDDY"
     gene            complement(3273..4901)
                     /locus_tag="PKV56_16575"
     CDS             complement(3273..4901)
                     /locus_tag="PKV56_16575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69476.1"
                     /translation="MRVADPEVQACRALVIDSNPTSRSTLINMLRDIGVGHVAQSSRV
                     EDARRELENRVFDIVVCDYHFDNSTISGQELLDDLRRAQLLPYSTVFVMVTGECSYVK
                     VAEAAESALDSYLIKPHTSAALEERVLQARYRKKVLKSIFEAIEASDFALAAQLCMAR
                     FEARGEFWLYAARIGAELFLRINDHKSAQALYAAVHESKALPWAKLGLARSELEAGQI
                     PKATRILESLIAEQPSYADAYDVMGRVQLEQGNLAAALDTYRESSMVTPQSITRLQKQ
                     GMLAFFMDQTDEAVNALDRTVRLGLSSKMFDCQSLVLLAMLYFDKRDAKEFKRIYALM
                     ELTVERRPDSIRLQRFLRTATVFQRLIARKVGECVSVARELASDLRREDFDYEAATNM
                     LAMLSRLRRTEVQLPDAEAWVSAVAQRFCVSKASCEMLCSAAGNDEVYAALIRSGQHD
                     ISTMAEKAMTHSVTGSPGAAVEALLLKGSETLNAKLIELAGAVLHRHAAKIDNNEAIR
                     LKIDDLKRRFCTKGTQVSLGQGSGRSAGGLTLRT"
     gene            complement(5125..>6132)
                     /locus_tag="PKV56_16580"
     CDS             complement(5125..>6132)
                     /locus_tag="PKV56_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOX69477.1"
                     /translation="FKEAKEAYEMLSDTQKRAAYDQYGHAGVDPNMGGAGFRPGGEGM
                     GGFAEAFGDIFGDIFGGGAAGGQRRGGGQQVFRGSDLSYAMEITLEEAAAGKETQIRI
                     PAYETCETCKGSGAKPGTSPKVCGSCNGSGAVTMRQGFFSIQQTCPTCRGAGKVIPEP
                     CTACNGQGRIKKTKTLEVKIPAGINEGMRIRSAGNGEPGTNGGPPGDLYIEIRVKQHE
                     IFERDGDDLHCTAPVSMITAALGGQVQVPTLTGKAEIELPEGTQHGKTFRLRGKGIKG
                     LRSSYPGDLYCHVTVETPIKLTEHQRKLLKELEESFQKGGDKHSPNSKSWSDRVKDLF
                     K"
BASE COUNT         1093 a         1941 c         1946 g         1152 t
ORIGIN      
        1 cgagtcgcgt gcgttcacgc gcagcctgcg cttcccgaag gccgactgac ggtcgcccga
       61 ctcctggttg gggcggccgc aggggctgga cccacggcta ctgaacacgg tccggacaag
      121 atatactcgc gggcttgcct tgccacaccc aggtcgacgc tcggggtggc ttccaatttc
      181 ggaattcaca aggagattca atgcgtcact acgaaatcgt gctgctgatc catccggatc
      241 agagcgaaca ggttccagcc atgctggaac gctacaaagg ggtgatcacc gccggcggcg
      301 gcaaggttca ccgggtcgag gactgggggc gtcgtcagat ggcctacatg atccagaagc
      361 tggccaaggc ccactacctc tgcatcaata tcgagtgcag caaggaagtt ctggccgaac
      421 ttgaaaccgg cttccgcttc aacgatgccg ttttgcgcca cctgaccgtg ctcaagagca
      481 aggccgagac ggcaccttcg gtgatgatga agaccagcga ccgtgaagac cgtcgcgcac
      541 cgcaggagtc tgctgcctga gttgtctgag tgatgtcggc ttgaccggac gcgcccgatg
      601 aacagactgg ttctgagggc agcagtggtc gagcgaggcg ctctgcgcta cacccccgcc
      661 gggttgccgg cactcgattt gagcctcagg cacgaatcgc aagtgacgca aaacggtatg
      721 ccgcgccagg tttcgatgga aatcaaggcg cgtgcggtgg gtgacatcac caaggcgttg
      781 aactgcatcg agctcggaag cacgcacggc tttgccggct tcctgggttc gcagcgcaac
      841 ggcaaaggca tcgttttcca tgtgaccgaa ctggactgaa cggtccacgg tcccgatctg
      901 aattgaattt gacagaggtt tgatatgcca ccaccacgcg gtaagggtaa gttttccaag
      961 gatcgcaagc cgaagcgcaa ccagcaatcg ctgttgttcc gccgcaagcg cttctgccgt
     1021 ttcaccgtca ccggtgtgac cgagatcgac tacaaggatg tcgagacact gcgcgacttc
     1081 atcggcgaaa acggcaagat cacgccggct cgcctgacgg ggacccgcgc cttcttccaa
     1141 cgtcagctga cgacctgcat caagcgggct cgtttcctgg cgatgctgcc gtacagcgat
     1201 cagcaccgcg tctgaggagc gacccatcat gcaaatcatc ctgctggaaa aagtggcgaa
     1261 tctgggcgac ctcggtgacg tcgtcaaggt caaggacggc tacgcccgca acttcctgat
     1321 tccgtcccgg tccgcacgtc gcgcgacccc gacggcgatc aaggaattcg aggtcaagcg
     1381 tgccgagctc gaaaaggctg ccgccgacaa gctggccgct gcacaggctc tgggcgagaa
     1441 gatgagcggt cgcaccgttc acatcaccca gaaggccggt gtcgacggac ggctgttcgg
     1501 ctcggtcacc aacaatgaca tcgccgaagc actgacccgc atcgacttca aggtggtcaa
     1561 gtcgcaggtc cgcctgccga atggcccgtt gaagacggtc ggcgagcacg ccgtgtcggt
     1621 gtcgccgcac acagatgtgg tggtcgaagt caaggtcgtg gtggtgggcg aaaccgcctg
     1681 acccgcgtct cttcgtccct cgcttcgaaa ggccggctct gccggccttt ttcattcgat
     1741 ttcccccggc attacccagc atctacacag ctttgtccac aggcgccggg accgagcgct
     1801 cgtatgatct tcagatgtca gcgctcctct cctcgcctca gctcacctcg caaccgcctc
     1861 ggggcgatga cgaagttgcc cggctccgaa tgccgccgca ttcgatcgaa gccgagcaga
     1921 gcgtgctcgg cggcctgctg ctcgacaaca gtgcctggga ccgcgccggc gacctgctga
     1981 ccgacagcga tttctatcga ttcgagcacc gggccatcta cgccgcgatc ggtgcgctgg
     2041 tcaacgccac ccgcccggct gacgtgatca cggtgttcga gcagctccag agcctcggca
     2101 aggccgagga atgcggcggg ctggtgtatc tcaacgcgct tgcgcagagc gtacccagcg
     2161 cagcgaacct gcgtcgctac gcggagatcg ttcgcgagcg cgcggttctg cgcaagctcg
     2221 tgtcggccag cgacgagatc gccaccagcg cattcaatcc gcagggcagg gcggtctcgg
     2281 agatcctcga cgaggcggag gccaagatat tcaagatcgg cgaggaaggc tcgcgctcgc
     2341 ggcagggttt ccagacgatg gattcgctgg tggtccagtt gctggatcgc gtgaccgagc
     2401 tgcacgagaa cggcgccgaa gaggtgaccg gcgtgcgcac gggttacttc gatctcgacc
     2461 gcatgaccgc aggtttccag cccggtgacc tgatcatcct ggccgcccgg ccgtcgatgg
     2521 gcaagacggc cctcgccctc aacattgcag agcacgtcgc cgttcaggag ggcctgccgg
     2581 ttgtcgtgtt ctcgatggaa atgggtgcgg cgcagctggc cttgcgactt gtcggatcgc
     2641 tggggcgcat cgaccagcag cacctgcgca ccggggcctt gaaggacgac gagtggggcc
     2701 ggttgaccga agcaatcgag aagctcggca gcgtcagcat gtacatcgac gagagcccgg
     2761 cactcacgcc gagcgagttg cgtgcgcggg cccgtcgcca agcccgtcag tgcggcaagc
     2821 tcggcctgat cgttgtcgac tacctgcagc tcatgagcgg cagcggcggt gccaacgagg
     2881 agaaccgcgc cacggtgatc ggagagatct cgcgaggctt gaagtcgctg gcgaaggaac
     2941 ttcaatgccc ggtgatcgcg ttgtcgcagc tcaatcgcag cgtcgagtcg cgcaccgaca
     3001 agcgtccgat gatgagcgac ctgcgcgagt ccggcgcgat cgagcaggat gccgatgtca
     3061 tcatgttcat ctaccgcgac gagtactaca cgaaggaggc ctgcaaggag ccgggtgtcg
     3121 ccgagatcat catcggcaag cagcgaaacg gtccgaccgg cacggtcaag ttgactttcc
     3181 tgaagccgct caccaagttc gacaacctgg caccgggcag cgccggatcc gacgactact
     3241 gatcgccggc agcccggcgt gtcgtgcgcc gctcaggtgc gcagtgtcaa cccacccgcc
     3301 gatcgtccgg aaccctggcc caggctcacc tgagttccct tggtgcagaa ccggcgcttg
     3361 aggtcatcga tcttgagccg gattgcctcg ttgttgtcga tcttcgccgc gtggcgatgc
     3421 aacacggctc ccgccaactc gatcagcttc gcgttcagtg tctcggatcc cttgagcagc
     3481 agcgcttcga cggcggcacc gggtgagccg gtgacgctgt gcgtcatggc tttttccgcc
     3541 atggtgctga tgtcgtgctg gccactgcgt atcagcgcgg catagacctc atcgttgccc
     3601 gctgcgctgc agagcatttc gcacgacgcc ttcgacacac agaaccgctg cgccactgca
     3661 ctgacccagg cttccgcatc gggcagttgt acctcggtcc tgcgcaggcg tgagagcatg
     3721 gccagcatgt tggtggctgc ctcgtagtcg aagtcctcgc gccgcaggtc gctggcgagc
     3781 tcgcgagcca cgctcacgca ctccccgacc ttgcgggcga tcaggcgctg gaatacggtg
     3841 gccgttcgca ggaagcgttg cagtcggatg ctgtccggcc ggcgctcgac ggtcagttcc
     3901 atgagcgcat agatgcgctt gaattccttg gcgtcacgtt tatcgaaata cagcatggcc
     3961 agcagcacca ggctctggca atcgaacatc ttcgagctga gccccaggcg caccgtgcga
     4021 tcgagcgcgt tcaccgcctc gtctgtctga tccatgaaga aggccagcat gccctgcttc
     4081 tgcaggcgtg tgatgctttg cggggtgacc atcgacgact cgcgataggt gtcgagagcg
     4141 gccgccagat tgccctgctc caactggaca cggcccatca cgtcgtaggc gtcggcgtaa
     4201 ctgggttgct cggcaatcag gctttcgagg atgcgcgtcg ccttcggtat ctgaccggct
     4261 tccagttcgg atcgcgccag ccccagcttg gcccagggca gggccttgga ttcatgaacc
     4321 gcggcgtaca acgcttgggc cgacttgtga tcgttgatgc gcaggaacag ctccgcgccg
     4381 atgcgtgccg cgtacagcca gaactcgccg cgggcctcga agcgcgccat gcacagctgg
     4441 gcggcgaggg cgaaatcact ggcttcgatg gcttcgaata tcgatttgag gaccttcttg
     4501 cgataccggg cctgcaggac ccgctcctca agcgctgcag acgtgtgggg cttgatcagg
     4561 tagctgtcga gcgcggactc ggccgcctca gcaaccttca cgtacgagca ttcgcccgtc
     4621 accatcacga agaccgtgga gtaaggcagc agctgtgccc gcctcaggtc gtcgagcagt
     4681 tcctggccgg agatggtcga attgtcgaag tggtagtcgc acacgacgat gtcgaacacc
     4741 cggttctcca gttcgcggcg tgcgtcctcg acgcggctgg actgcgcgac atgtccgacg
     4801 ccgatgtcgc gcagcatgtt gatgagcgtg ctgcgcgagg tcgggttgct gtcgatcacc
     4861 agcgcacggc aggcctgcac ctcggggtcg gcaactctca tggtgcggcc cccgatgggt
     4921 gaacatcctg cacatcaggt ttggcgatgc gcgcagaagc cgccgtgcgc agtccacgaa
     4981 gccgcggggg cgctgcatcg gtcgggtgta acgatcgcaa tccaggcata gacatccgat
     5041 atccagggga accccaggta tatcggacgg ctggatgctg gcttgaggct ccggggaagc
     5101 ggtttgcccc ggagcgcggg cctgctactt gaagaggtcc ttgacccggt ccgaccagga
     5161 cttcgagttc ggggagtgct tgtcgccgcc cttctggaaa gactcctcga gctccttgag
     5221 cagcttgcgc tggtgctccg tgagcttgat cggcgtctcg accgtgacat ggcaatagag
     5281 atcgcccggg tagctggagc gcaggccctt gatgcccttg ccacgcagtc ggaaggtctt
     5341 gccgtgctgc gtgccttcgg gcaactcgat ctctgccttg cccgtcagcg tgggcacttg
     5401 cacctggcct ccgagcgccg ccgtgatcat gctcacgggt gccgtgcagt gcaggtcgtc
     5461 tccgtcacgc tcgaatatct cgtgctgctt gacccggatc tcgatgtaga ggtcgccggg
     5521 cgggccgccg ttggttcctg gttcgccgtt accggccgat cggatgcgca tgccttcatt
     5581 gatgccggcc ggaatcttga cttcgagggt cttggtcttc ttgatgcgcc cctggccgtt
     5641 gcaggcagtg cagggctccg ggatgacctt gccagcgccg cggcaggtcg ggcaggtctg
     5701 ctgaatgctg aagaaaccct ggcgcatcgt caccgcgccc gagccgttgc aactgccgca
     5761 caccttcggg ctggtgcccg gcttggcgcc gctgcccttg caggtttcgc aggtctcgta
     5821 ggcaggaatc ctgatctggg tttccttgcc tgccgcggct tcctccaggg tgatttccat
     5881 ggcatacgac aagtcgctgc ctcggaacac ctgctgaccg cctcctctgc gctgaccacc
     5941 tgcggcgccg ccgccgaaga tgtcgccgaa gatgtcgccg aaggcttcgg caaacccgcc
     6001 catgccttcg ccgccgggac ggaatccggc gccacccatg ttcgggtcga cgcccgcatg
     6061 gccgtattga tcgtaggcag cacgcttctg cgtgtccgag agcatctcgt aggcttcctt
     6121 ggcctccttg aa
//
LOCUS       DAOORV010000189         6097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15715_length_6097_cov_9.655909, whole genome shotgun sequence.
ACCESSION   DAOORV010000189 DAOORV010000000
VERSION     DAOORV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15715_length_6097_cov_9.655909"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..417
                     /locus_tag="PKV56_16585"
     CDS             166..417
                     /locus_tag="PKV56_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOX69478.1"
                     /translation="MGQFTVRNIEDEVKDRLKRRAASHGVSMEAEVRQILRNAVKDDD
                     LAAGMGLGTRIAARFAGDGLNEPLPELKGQGPQAITLAA"
     gene            414..830
                     /locus_tag="PKV56_16590"
     CDS             414..830
                     /locus_tag="PKV56_16590"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOX69479.1"
                     /translation="MIILDTNVLSELMRRQPAPAVLRWLDLQPSQSVWITTITLFESR
                     LGLALLPPGQRRESLAQAFDQLLAIDLENRVLPFDAEAAAQAALLAADRQRNGRPVDI
                     RDTQIAGIAQARRATLATRNLRHFEGLTVAVVDPWV"
     gene            1007..1300
                     /locus_tag="PKV56_16595"
     CDS             1007..1300
                     /locus_tag="PKV56_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69480.1"
                     /translation="MRTFFQKPIAILTLRWRVAELLDAGLAALTPDTALDDGGQRIAI
                     TRAPAMNLRLIVCDAPTSALGVNLQAKVLNLPRDLRRELGVSDLFITHNIAVA"
     gene            1825..4677
                     /locus_tag="PKV56_16600"
     CDS             1825..4677
                     /locus_tag="PKV56_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX69481.1"
                     /translation="MFNRTKINTGALLALGLIASLPALAQQRVEITGSNIRRAQAETA
                     SPVQTVTREEIERSGKTSVAELLQTLAVDNQGSVPTTFGSGFASGASGISLRGLGAAS
                     TLVLVNGRRIAPYGLADDGQKVFADLNMIPLEAVERVEVLKDGASSIYGSDAIAGVVN
                     VILRKDFVGTALKASAGQSRYQDGRNLVATVTHGFGNLEKDKFNFLVNFEVTKKDEIY
                     NRDRAGRGVVGRSDLRSIGFDAVNSGGANGGTGAILDNNTAVSSTVGNVRNPTTSNYY
                     SRSQLDGVGFTRTFPGANCSNFSKIPQGDPGFAGGIGCLTDAAQAYGMVQPSLESLNL
                     FARGTMQLTPQVQGYVEANWYKNESFSQTTPSGVNGNAGYPGGPVSNASVSLAASHPD
                     NPYFGSAARVRYQAADVGPRVSNVDSTFVRLVAGAKGNFGSWDFDSAVLHSESKVSNS
                     RTGYLQRDATFALLNPGGANALVAGKTNAEVAAANNPGYAALPAGTFWRIAENAGLNS
                     AALYGALSPTISNDAKSKTTQVDLKVSRDLMSLAGGMMGLAMGAEVRRESISLEPTSG
                     TERGNIIGLGYSAYSGSRTLTAAYAEVLAPVSKQLELSAAVRADHYSDVGNSFTPKLG
                     MKFTPMRELALRATYAEGFRAPSAAENGKGGLAAFSTAADPLRCNLGIQTACSASSVA
                     VITSPNPALKPEKSKSYTLGLVFEPMPGTSASVDFWQIVRKGEINQEATSAAIAAGKV
                     SRDPSTATATPGDPGGITAVLANYVNSSQSTVRGVDLDLRQSFGMGSSGKLDFDLKWT
                     HLFKFERQELDGSVVNFAGTHGNCDVTNCIGTPADRANLGVTWTAGPWRVGAVANYRA
                     SISGKDSKESTDCPTLYADGTDAPAGCRLASFTTVDLTVRWKASNNLEVFGSIQNLFD
                     RIPPVDAHTYGATSYNPLDYSGAVGRYYSVGLKYKF"
     gene            complement(4799..5122)
                     /locus_tag="PKV56_16605"
                     /pseudo
     CDS             complement(4799..5122)
                     /locus_tag="PKV56_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(5140..5565)
                     /locus_tag="PKV56_16610"
     CDS             complement(5140..5565)
                     /locus_tag="PKV56_16610"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX69482.1"
                     /translation="MRSFLDTNVLVYADAADEPVKQATAIALISRLRREGTGVISTQV
                     LQEFANVALRKLRLPHALVRERLVFFAGFDVTRSSPELIQSALDLHIAHGLAFYDALI
                     VQAALDSGCQQLWSEDLQTGRLLGKLRISNPFASVPTWE"
     gene            complement(5580..5807)
                     /locus_tag="PKV56_16615"
     CDS             complement(5580..5807)
                     /locus_tag="PKV56_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX69483.1"
                     /translation="MNITLSIDERVAERARQAAQAMGKSLNQAVRDHLEQLAGMTQQD
                     AQAEAFEASARASPGRLNGWQFDRDEANARR"
     gene            complement(5889..>6097)
                     /locus_tag="PKV56_16620"
     CDS             complement(5889..>6097)
                     /locus_tag="PKV56_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOX69484.1"
                     /translation="TIAPGIFGTPMLFTMPQAVQDALAAGVPFPSRLGTPADYAKLVK
                     HIIENDMLNGEVIRLDGAIRLAPK"
BASE COUNT         1147 a         1841 c         1901 g         1208 t
ORIGIN      
        1 tcgagcaggg cgcctgtgct gatgtgctgc agcggccggt ggatgactac acgcgcaacc
       61 tgctggcggc ggtgcccagg ctctagccgt tgacagcggc gctgagcaac cagcccgccg
      121 cgatgttgca ccgattgata tcatgatatt tttcaaggga cgatcatggg tcaattcacc
      181 gttcgcaaca tcgaagatga ggtcaaggat cggctgaaac ggcgcgcagc gtcccatggc
      241 gtcagcatgg aggccgaggt tcggcaaatc ctgcgcaacg cggtcaagga tgacgatctg
      301 gctgccggta tggggctggg cacccggatc gccgcccggt ttgcggggga cggtctgaac
      361 gagcccctgc ctgagttgaa gggccaagga ccccaggcca tcacactcgc ggcatgatca
      421 ttcttgacac caacgtgttg tcggagctga tgcggcgcca gcccgcgcct gccgtgctgc
      481 gttggctgga cctgcagccg tcccagtcgg tctggatcac caccatcacc ctgtttgaaa
      541 gccgacttgg actggcgctg ttgccgccag ggcaacggcg ggagagcttg gcgcaggcct
      601 ttgatcagtt gttggcgatc gaccttgaga accgtgttct gccgttcgac gcagaggctg
      661 cggcacaagc cgcgctgctg gcggccgacc ggcaacgcaa cggtcgtccg gtggacatcc
      721 gcgacactca aattgccggc attgctcagg cgcgacgtgc cacgctggcg acgcggaacc
      781 tgcggcactt tgaagggctg actgtggcgg tggtcgatcc ctgggtctga gcgcgaacgc
      841 tgcccggggc gcccaggcga ggcgggtcgg gctgcggcat gaccaccagc ggccaggcca
      901 ccttgaaact gttgcgtggt caggtccaga tcgatgccca gccgttgttg aacggccgca
      961 acaaggtcga ccggaatggg ccgagctgcg caacgccttt cgctgggtga ggaccttctt
     1021 ccagaagccg atcgctatcc tgaccctgcg ctggcgggtg gccgagctcc tggacgcggg
     1081 cctggccgcg ctgacgcctg acaccgcgct ggacgacggc ggccagcgca tcgccatcac
     1141 ccgtgcgccg gcgatgaacc tgcggctgat cgtctgcgac gcgcccacct cggccctggg
     1201 cgtcaacttg caggcgaagg ttctgaacct gccgcgcgac ctgcggcgcg aactgggcgt
     1261 cagcgacctg ttcatcaccc acaacatcgc ggtggcgtag cacattgccg aacacgtggc
     1321 tgtgatgcgc gcggaacgca tcgtcgagca gggcgcctgc gccgatgtgc tgcagcgccc
     1381 ggtgcatgac tacacccgtg cgctgtaggt tgcggtgccg gcggcaccgc gtagcggcga
     1441 agtcaatcgt ttggcatgaa ctcgccggca gccgctgcga tcaggcctgt tgcagacaac
     1501 caacggagtg ttcgggcggt tctaggcggg gccacctaga gccgcggact gctagtgtgt
     1561 gccgccggcc tcgaatgcct cgcaagttgc tgtttttgat gatcttttcg gtcaacagcg
     1621 gtgcatgaca gcgccacagg gcggcgcgtg tccggtgcta ggtgattcga cgagggtttc
     1681 cctagagcgg cgtccggtgg gatgacagag acaatcgcga tacacgttgc tcgtacccac
     1741 ccaccagcag tttgcgaaac catccaaacg gcgatgacgg gcttatcggt ctacggaccc
     1801 atttgttttg aatcaggagc tttcatgttc aacaggacca agatcaacac aggcgcactt
     1861 ctggcgctgg gcctcatcgc ctcccttccc gcactcgctc agcagcgcgt cgaaattacc
     1921 ggttcgaaca ttcggcgtgc gcaggccgaa acggcatcac cggtgcagac cgtgacccgc
     1981 gaggaaatcg agcgttcagg caagaccagc gttgctgagt tgctgcagac gctggccgtg
     2041 gacaaccagg gctcagtgcc cacaactttc ggcagtggct ttgcgtctgg cgcttcgggc
     2101 atctcacttc gaggcctggg tgccgcttca acgcttgtgt tggtcaacgg ccggcgcatc
     2161 gcgccgtatg gcttggctga tgacggccag aaggtctttg cagacctgaa catgataccg
     2221 ctggaagcgg ttgagcgtgt tgaagtgctg aaggacggcg cgtcatcgat ctacggctct
     2281 gacgctattg ccggcgtggt gaacgtgatt cttcggaaag attttgtcgg cacggccctg
     2341 aaggcctcgg caggccaatc tcgctaccaa gacgggcgca acttggttgc gactgtgacg
     2401 catggctttg gcaatctcga gaaagacaag tttaactttc tcgtgaattt tgaagtgacg
     2461 aagaaggacg agatctacaa ccgcgaccgc gccggccgcg gtgtggtagg tcggtccgat
     2521 ctgcgaagca ttgggtttga cgcagtcaac agcggaggtg ccaatggcgg cacaggggcc
     2581 atcctcgaca acaacacggc agtatcttcc accgtcggta atgttcgcaa tcccaccaca
     2641 tcaaactact atagccgtag tcaactcgat ggcgtgggct tcacaaggac gtttccaggc
     2701 gcgaactgca gcaatttcag caagattccc cagggcgacc caggttttgc cggcggtata
     2761 ggctgcctga cggacgcggc ccaggcatac ggaatggtgc aaccttcgct cgagtcactg
     2821 aacttgttcg cgcgcggcac tatgcaactc acaccgcagg tccagggcta tgtggaagcc
     2881 aactggtaca agaacgaatc cttttcgcaa accaccccgt caggggtcaa cggaaatgcc
     2941 gggtaccccg gcggtcccgt tagcaacgcc tccgtctcac tggctgcgag ccatcctgac
     3001 aacccgtact ttgggtctgc cgcccgtgtg cgataccaag cggccgatgt tggcccgcgc
     3061 gtgagcaacg tcgattccac ctttgtccgc ttggttgctg gcgcaaaggg taactttggc
     3121 agctgggact ttgattcagc ggtactgcac tccgaaagca aggtctccaa ctcacgcaca
     3181 ggctaccttc agcgtgacgc tacgtttgca ctgttgaatc caggcggagc aaatgccctt
     3241 gtcgcaggca aaaccaatgc tgaagtggcg gcggcgaaca accctggata cgctgcactg
     3301 ccagctggga ctttctggcg catcgctgaa aacgctggcc tgaattcggc ggcgttgtat
     3361 ggcgctttgt cgccgacgat atccaacgac gcaaagtcca aaaccacaca ggttgacttg
     3421 aaagtttcgc gtgacttgat gtcgctcgct ggcggtatga tgggtttggc catgggtgcg
     3481 gaggttcgcc gtgagtcgat cagtcttgag ccgacttcgg gcacagaacg aggaaacatt
     3541 atcggtcttg gctactctgc ctactcaggt tcacgcaccc tgacggctgc ttacgccgaa
     3601 gtgttggcac ctgtgagcaa gcaattggag ttgtccgctg cggttcgagc cgaccactac
     3661 tcagacgtgg gcaactcatt tacgccaaag ctgggcatga agttcacgcc catgcgggaa
     3721 ctggcgcttc gtgcgacgta cgctgaaggc tttcgagcgc cgagcgctgc cgagaacggc
     3781 aagggcggat tggcggcttt ctcaacggcc gctgacccac tgcgatgcaa tcttggcatc
     3841 cagacagctt gttcggcgtc gagcgtggct gtgattactt cgcccaatcc tgcgttgaag
     3901 cccgaaaagt ccaagagcta tacgcttggc cttgtgtttg aaccgatgcc cggcacgagt
     3961 gcttctgttg atttctggca gatcgtccga aaaggtgaaa tcaatcaaga agccacatcg
     4021 gctgctatcg cggcgggcaa agtgtctcgt gacccatcga cggcgacagc aacaccaggc
     4081 gacccgggag gaatcactgc tgttttggcg aattacgtaa attcttcgca gtcaacagtt
     4141 cgtggcgtgg acttggacct gcgtcaatct tttggaatgg gttccagcgg caagctggac
     4201 tttgacctca agtggacgca tctcttcaag ttcgagcgcc aagaactcga cggatcggtg
     4261 gtgaactttg cgggcacaca cggcaactgt gatgtgacga actgcattgg aaccccggcg
     4321 gacagggcca acctgggtgt gacatggact gcgggcccat ggcgtgtcgg tgcagtggcc
     4381 aattaccgcg catcgatatc gggcaaagac tccaaggagt cgaccgattg cccgaccctt
     4441 tatgcggacg gtacagacgc gcccgcaggg tgccgtctgg cttctttcac gacagtcgat
     4501 ttgactgtgc gctggaaggc atccaacaat ttggaggtgt ttgggtcgat tcaaaacctg
     4561 tttgatcgca ttccccccgt tgatgcccat acctacggcg ccacgtctta caacccgctg
     4621 gactacagcg gcgcagtggg ccgctactac agcgtcggcc tgaagtacaa gttctaaaat
     4681 tcagcccgtt gcgagcccac tgtccttcgg gacagtgggc ttttgtcctt tggcgcgtcg
     4741 atcctcgtgc tagggatgcg gttgcgcagc agatcaccag ttttgtgggg ccgcctccct
     4801 tcggcgccag ccgaaacgcc ccgtcgagcc ggatcacctc gccgttgagc atgtcgttct
     4861 cgatgatgtg cttgaccagc ttggcgtagt cggccggcgt tcccaggcgg ctggggaagg
     4921 gcacaccggc agcgagtgcg tcctgcaccg cttgcggcat ggtgaacagc atcggcgtgc
     4981 cgaagatgcc cggggcgatc gtcatcttgc ggatgccgtt gcgcgccagg tcgcgggcga
     5041 ttggcaatgt catgccaacc acgccgccct tgctggcggc atacgcggcc gatccgatct
     5101 ggccttctct cgccgccacc gcacgtcgcc cccgcggcat cactcccagg tcggcacgct
     5161 tgcaaacggg ttgctgatgc gcagcttgcc cagcagccgc cccgtctgca ggtcttcgct
     5221 ccacagttgc tggcaaccgc tgtccagcgc ggcctgcacg atcagcgcgt cgtagaaggc
     5281 cagtccgtgg gcgatgtgca agtcaagggc cgactggatc agttcgggcg agctccgggt
     5341 gacatcgaag ccggcgaaga acaccagccg ctctcttacc agcgcatgcg gtagccgcag
     5401 cttccgcaag gccacgttcg cgaactcttg caatacctgg gtcgagatca cgcctgtgcc
     5461 ctcccggcgc aggcggctga tcaaggcgat ggcggtcgct tgcttgaccg gctcatccgc
     5521 agcgtcggcg tagaccagta cgttggtgtc caggaaactg cgcatcggcg gcctcgacat
     5581 catctcctgg cattggcctc atcccggtca aactgccagc cattcaaacg gccggggctg
     5641 gcgcgggcag aggcttcaaa tgcttcggct tgcgcatctt gctgcgtcat gccggcaagt
     5701 tgctccaaat ggtcacgcac ggcctgattc aaactcttgc ccatggcctg cgccgcttgg
     5761 cgtgcacgct cagcaacgcg ctcatcgatg gacagggtga tgttcatgga agcctgatgc
     5821 cagcaagtaa tttgtgcaca cagtttacgt gtcaatcagc actttggtca agaacgctgg
     5881 cgcgcgcatc acttcggcgc cagccggatc gccccgtcca gccggatcac ctcgccgttg
     5941 agcatgtcgt tctcgatgat gtgcttgacc agcttggcgt agtcggccgg ggtgcccagc
     6001 cggctgggaa agggcacgcc ggcggccagc gcgtcctgca ccgcctgcgg catggtgaac
     6061 agcatcggcg tgccgaagat gcccggggcg atggtca
//
LOCUS       DAOORV010000190         6086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15758_length_6086_cov_8.592936, whole genome shotgun sequence.
ACCESSION   DAOORV010000190 DAOORV010000000
VERSION     DAOORV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15758_length_6086_cov_8.592936"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKV56_16625"
     CDS             complement(<1..478)
                     /locus_tag="PKV56_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HOX69485.1"
                     /translation="MKSGRTLVSLAQELERQLHSKKDLVVPSQLVHHSTGDGGETHLV
                     IDEGGGAVHYGVMPLARRQLADKLKIPYAYFERMREEQPVLLDRNVNTWLQSEDDRRM
                     LRTLDGKVRAVLSDRYRRLDNFDLAESVLPILQQLPEVRFESVELTETRMYLKCITS"
     gene            complement(779..1831)
                     /locus_tag="PKV56_16630"
     CDS             complement(779..1831)
                     /locus_tag="PKV56_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOX69486.1"
                     /translation="MHDHEYAGRIDAVKQRAHGRWSEILASAGVEEQILKHRNGPCPL
                     CGGTDRFQFTDKFGEGNYHCRQCGPGGGFKLLQAVKGVDFNAALRDVERCLGLLPEAT
                     TAGAGEPSGDRMRKLVQRIWDEARPVTAGDEVDRYLRGRGLALPVVPAVLRFHPALGY
                     YEKDGAGKSRKLAEYPAMLACIQGVDGHAVTLHRTYLKDGAKLKAADAKKVLSAGING
                     AAVRLFEATDELAVCEGIETGLALHLATRKPVWAGINAGNLEKLCLPDTVRQVCIYAD
                     NDADGDFAGQCFAFALARRLKREEKTTGARQVRVFVPRHAGTDWADVWCQRRKVSSAP
                     MSAPEPMPGVGEQVSE"
     gene            complement(1818..>3011)
                     /locus_tag="PKV56_16635"
     CDS             complement(1818..>3011)
                     /locus_tag="PKV56_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69487.1"
                     /translation="VEELCLGFPVAVLFNGQPLKRPLAEGHLATQASPIGAVHLAGTL
                     DGKYSHETMVFLQGFCVLKPTYCLRDELNVVHLDSRQFMARLPDRDKLIDEDVQRKRI
                     DAELKACWRNTLEIARTRLSPERFVETYYAVMRGWGQLDLLNDLDVLPAELCDAIVGY
                     PIQDDHGSREFVQQVAAAPTRSDIESGAVRLVALDCVGDENGARWMLARSKGYLVFDW
                     IGLHHEHWAQRHVRFLEEERVHVEAVNEQVRTALEGRWVWPDVILCEAVRIRVGKDEA
                     MIIDEGVCHGGTLYIPTGESTGTPVQQLSSFTDEHDQFLDADCDADRDALADLIRRLR
                     SVDPVQTLDSLLQDLRLGKYPLLHGKIFQLTVGVGVSPGHSIELLEAEGLAVSAESGG
                     RHARS"
     assembly_gap    3012..3111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3112..3344)
                     /locus_tag="PKV56_16640"
     CDS             complement(<3112..3344)
                     /locus_tag="PKV56_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69488.1"
                     /translation="MQIQVRINEEGALRNQRYAFTNRFTLVSELLQNARRAGAACIEI
                     HYDAATQVLSVHDDGCGLEDFQKLLSFHESGWDA"
     gene            complement(3422..3760)
                     /locus_tag="PKV56_16645"
     CDS             complement(3422..3760)
                     /locus_tag="PKV56_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69489.1"
                     /translation="MFTFMNALRIGPDAPRLLTVQAPEDIEYLVKESEVLTGQAGRTF
                     VISGADWLVYRVSFCERTHPSGLKVERLDAHGRVVSTQHLQVWEFLDHSLVEAQAAGQ
                     LFTPPVRRHG"
     gene            complement(3822..4388)
                     /locus_tag="PKV56_16650"
     CDS             complement(3822..4388)
                     /locus_tag="PKV56_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69490.1"
                     /translation="MLQANASPGAFRPESAGHRFHPFHGVSPQHPTARPDGIADEALP
                     DDEAAVVEWSEDDIVFLHWRLLQEVRHLADPATPLEEKLDTLRWVFTEREKDAAPFSF
                     VSCLRVVGCSPLSPIAYCGLVDAEEVRDRIRNGLKAWLNATLERYPEWVRSAVAGNPE
                     WVEARLARNPQWINEQLKQMSVQGDLFA"
     gene            4942..5697
                     /locus_tag="PKV56_16655"
     CDS             4942..5697
                     /locus_tag="PKV56_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1845 domain-containing protein"
                     /protein_id="HOX69491.1"
                     /translation="MPDIEIVKLDQGGVNARILAKEAKVDFRRVEAASLKMPTRLTSA
                     EGKRSFVRMFNTLQLNTHFISVIARTRLDHDDIGKIEAAIRAQMDAATASLNKAIDGA
                     EALFKAHGITSAASYDTVPLDVEVHVLSSASRRFLEVLGKLDQLMPLLQTLEIHEVIT
                     AQAVDIQRAGLKRQVRDIANGARNFAIGLRRRMNALDARNGESAREDVTDAETEQSPE
                     SSTEGGVDVMREAAAAPMVTSEAAAELPIAVEE"
BASE COUNT         1012 a         1996 c         1992 g          986 t
ORIGIN      
        1 gcgaggtgat gcacttcagg tacatgcgcg tctcggtcag ctcgaccgac tcgaaccgca
       61 cctcgggcaa ctgctgcagg atgggcagca cgctctcggc gaggtcgaag ttgtccaggc
      121 ggcggtagcg gtcggacaac accgcacgca ccttgccgtc cagcgtgcgc agcatgcgcc
      181 ggtcgtcctc gctttgcagc caggtgttca cgttgcggtc cagcaggacc ggttgctcct
      241 cgcgcatgcg ttcgaagtac gcatacggga tcttcagctt gtcggccaac tggcgacgcg
      301 ccaacggcat gacgccgtaa tgcacggcgc cgccgccttc gtcgatgacg aggtgggtct
      361 cgccaccatc gcccgtgctg tggtgcacga gctgcgaggg cacgaccagg tccttcttcg
      421 agtgcaactg gcgctccagt tcctgggcca ggctcacgag agtacgtccg cttttcatat
      481 caaactcctt gtgggatgaa gaagcgggga cggcacgacc cccgttgcgg ggatgcgcat
      541 ccccgcaagg gctgatgacg gaccggctgc accttggcag accggtccac gacgtgcgcg
      601 aatcgaatcg cgcggaaaac tccagggctg tcttcgcgac gccgtgaggc gccgcctggt
      661 ttgacaacta ccagtggggg aactcgtcga agccttttcg ccggcaccgg gtcgaatcga
      721 tccagtgccg ccgaaaaagc gggtgcggcc ccgtcaacat gaccggccgc accccacatc
      781 actcactcac ctgttcaccg acaccgggca tcggttccgg ggcggacatc ggcgccgatg
      841 acaccttccg gcgctggcac cacacatcgg cccagtcggt gccggcgtgg cgcggtacga
      901 acacccgcac ctgccttgcg ccggtcgtct tctcttcgcg cttcaaccgg cgcgccaatg
      961 cgaaggcgaa gcactggccg gcgaaatcgc cgtcggcgtc gttgtcggca tagatgcaca
     1021 cctggcgcac cgtgtccggc aggcacagct tctccaggtt gccggcgttg atgccggccc
     1081 ataccggttt gcgcgtggcc aggtgcagcg cgaggccggt ctcgatgcct tcgcagaccg
     1141 ccagttcgtc ggtcgcctcg aagaggcgga ccgcagcgcc gttgatcccg gccgagagca
     1201 ccttcttggc atccgccgcc ttcagcttcg ccccgtcctt caggtaggtg cggtgaagcg
     1261 tcaccgcatg cccgtccaca ccctggatgc aggccagcat ggccgggtac tcggccagct
     1321 tgcgtgactt accggccccg tccttctcgt agtagcccag cgccgggtga aaccgcagca
     1381 cggccgggac caccggcagg gccagcccgc ggccgcggag gtagcgatcg acctcgtcgc
     1441 cggccgtcac cggccgggct tcgtcccaga tgcgctgtac cagcttgcgc atgcggtcgc
     1501 ccgagggctc gcccgcgccc gccgtcgttg cctcgggcag cagacccagg cagcgctcaa
     1561 catcgcgcag cgcggcgttg aagtccactc ccttgacggc ctgcagcagc ttgaagccgc
     1621 caccgggccc gcactggcgg cagtggtagt tgccctcgcc gaacttgtca gtgaactgaa
     1681 accgatccgt tccgccgcac agcgggcagg gcccgttgcg atgcttcagg atctgctcct
     1741 ccacgcccgc gctggcgagg atctcgctcc accggccatg cgcccgctgc ttgaccgcgt
     1801 cgatgcggcc ggcgtactca tgatcgtgca tgacggcctc cgctctctgc cgacacggcc
     1861 agcccttcgg cctccagcag ttcgatggaa tggccaggcg acacgcccac gcccacggtc
     1921 aattggaaga tcttgccgtg aagcagcggg tacttgccca gccgcaggtc ctgcagcaac
     1981 gagtcgagcg tctgcacggg gtcgaccgag cgcaggcgac ggatgaggtc ggccagcgcg
     2041 tcacggtcgg cgtcgcaatc ggcatcgagg aactgatcgt gctcgtcggt gaaggacgag
     2101 agttgctgca ccggcgtgcc ggtgctttcg ccggtgggga tgtacagggt gccgccgtgg
     2161 cacaccccct cgtcgatgat catggcctcg tccttgccga cccggatccg tacggcttcg
     2221 cacaggatca cgtcgggcca gacccagcgt ccctccagcg cggtgcgcac ctgttcattt
     2281 accgcctcga cgtgcacgcg ctcttcttcg agaaagcgaa cgtggcgctg cgcccagtgc
     2341 tcgtgatgca ggccgatcca gtcgaacacc agatagccct tgctgcgggc cagcatccag
     2401 cgtgcgccgt tctcgtcccc gacgcagtcg agggcgacca gccgcacggc gccgctctcg
     2461 atatcgctgc gcgtcggcgc ggcggccact tgctggacga attcgcgtga accgtggtcg
     2521 tcctggatcg gatagccgac gattgcatcg cacagctcgg ccggcaacac gtccaggtcg
     2581 ttcagcaggt ccagttgccc ccagccgcgc atcaccgcgt agtaggtctc gacgaagcgc
     2641 tccggcgaca gccgggtcct cgcgatctcc agcgtgttgc gccagcaggc cttcaactcg
     2701 gcatcgatgc gcttgcgctg cacgtcctcg tcgatcagct tgtcgcggtc gggcagccgt
     2761 gccatgaact ggcgcgagtc cagatgcacg acgttgagct cgtcgcgcag gcagtacgtc
     2821 ggcttgagga cgcagaagcc ctgcaggaac accatcgtct cgtggctgta cttgccgtcg
     2881 agcgtgcccg ccaggtgcac cgcaccgatg ggactggcct gcgtggccag gtgcccttcg
     2941 gccaagggcc gcttcagcgg ctggccgttg aacagcacgg ccaccgggaa ccccaggcac
     3001 agctcctcga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgtcccag
     3121 cccgattcgt gaaaactcag cagcttctgg aagtcctcca ggccgcaacc gtcgtcgtgg
     3181 acgctcagca cctgagtcgc cgcgtcgtag tggatctcga tgcacgccgc gccggcgcga
     3241 cgtgcgttct gcaacagctc ggagacgagc gtgaagcggt tggtgaaggc gtagcgctgg
     3301 ttgcgcaggg cgccttcctc gttgattcga acctgaatct gcatgacatg ctccatgggt
     3361 ggaggcgggc cccgtcccca gcgggacaag cccgccggat ggggggaatg gacgaattcg
     3421 gtcagccgtg tcggcgaacc ggtggggtga acaactggcc ggcggcctgg gcctcgacca
     3481 ggctgtggtc caggaactcc cagacctgca ggtgctgggt gctcacgacg cggccatgcg
     3541 cgtcgagccg ctcgaccttc aggccgctcg ggtgcgttct ttcgcagaag ctcacccggt
     3601 agacgagcca gtcggcgccg gagatgacga aggtgcgtcc ggcctggccg gtgagcactt
     3661 cgctttcctt gaccaggtac tcgatgtctt ccggggcctg cacggtcagc aggcgtggcg
     3721 catcgggacc gatgcgcagg gcattcatga acgtgaacat gaacaactcc ttgaacggga
     3781 tgggctggcg cttcttcgcc gggcccgggt gggggaaggg atcaggcgaa caggtcgcct
     3841 tgcacggaca tctgcttgag ctgctcgttg atccactgcg gatttctggc caagcgggcc
     3901 tcgacccact cggggttgcc ggccacggca ctgcgcaccc actcggggta gcgctccagg
     3961 gtggcgttga gccaggcctt caggccattg cggatgcggt cgcgcacctc ttcggcgtcg
     4021 acgaggccgc agtaggcgat cggcgacagc gggctgcagc cgacgacgcg caggcagctg
     4081 acgaaggaga agggcgctgc gtccttctcg cgctcggtga acacccagcg cagggtgtcg
     4141 agcttctctt ccagcggcgt ggccgggtcg gcgaggtgcc gcacttcctg cagcagccgc
     4201 cagtgcagga agacgatgtc gtcttcgctc cactcgacga ctgccgcctc gtcgtcgggc
     4261 aatgcctcat cggcgatgcc gtcggggcgg gcggtcgggt gctgtggcga gaccccatgg
     4321 aaggggtgga accgatgccc agcggactct gggcggaatg ctccggggga tgcgttggct
     4381 tgcagcatcg ttgactccat ggaaaggcag gaggcaacgc gcaccacggg tgggggcgag
     4441 agcccccagg gggtgacgaa caaccggcgc tgagaccggc gctatgggag ggtgactcca
     4501 gggctggctt ggcttggcgg tgcgaaccgc cttgatcggt gcgccagcta ccgatcgagg
     4561 aaagccttgg agggaagggc gcggcgacaa cgcgcagccc ttgcctcgaa ggcttgcttc
     4621 gacggcgagc gaaagactga caccggcgac ggtgccgtca ggctcggagc acggcgcgat
     4681 cgtgtgagat cccaggacgg ggcatcacag ccatcgcaat ccatgcgtca gacagaaaac
     4741 tccagggctt gccttgtttg aatgcagggc cagctgctgc atgacgcaaa ggtagaccgg
     4801 cgccagcact aggtcaatgc cgaaacgccg acgatccggg cctgatcccg aaagcacccg
     4861 caagtgccgg ccatatcgcg tggcgattgc gcacggtgat cgggaagatg gctccgacca
     4921 tcgcctttgc aggagcacgg catgccagac atcgagatcg tcaagttgga ccagggcggc
     4981 gtcaatgccc gcatcctcgc gaaagaggcc aaggtcgatt tccggcgcgt cgaggcggcc
     5041 agcctgaaga tgccgacgcg cttaaccagc gccgagggca agcgctcctt cgtgcggatg
     5101 ttcaacacgc tgcagctcaa cacgcacttc atctcggtga tcgcgcgcac ccggctggac
     5161 cacgacgaca tcggcaagat cgaggcggcg atccgggcgc agatggatgc ggccaccgcc
     5221 agcctgaaca aggccatcga cggtgcggag gcactcttca aggcccatgg catcaccagc
     5281 gcggcgtcct acgacaccgt gccgctggac gtcgaggtcc acgtgctgtc gtctgccagc
     5341 aggcgcttcc tggaggtgct gggcaagctc gaccaactga tgccgctgct gcagacgctg
     5401 gagatccacg aggtgatcac ggcgcaggcc gtcgacatcc agcgtgcggg tctgaagcgc
     5461 caggtgcgcg acatcgccaa cggcgcacgc aatttcgcga tcggactgcg gcgacgcatg
     5521 aacgccctgg atgcgcgcaa cggtgagtcg gcacgagagg acgtcaccga cgccgaaacg
     5581 gagcagtcac ccgagagttc gacggaaggc ggcgtcgacg tcatgcgcga ggccgctgcc
     5641 gcgccgatgg tgacgtcgga ggcggcagca gagctgccca tcgccgtcga ggaatagccg
     5701 cgggcaaggt ccgccacgcg caatcggcgc tctttccagg tggtttcggt ggcaaccttc
     5761 gaggacgtct tgctgcgacc tgcgagattg aatacggtgt agccaggttc catgctgtcg
     5821 gcgcctgctg tacgtgaccg gagcctccgc ccgctgtcgg gcggcgtcgc agtcgatacc
     5881 tgcctcttgc accccgaccc gcgtgcgcat cgaacttcgg cagacgcacc gccggatcgc
     5941 aagtcatcgg aatcagcagg ttgccgagcg tcacgacgct tgtggcaccg tcatctgcaa
     6001 accaccacgc cgcgataccg ggacgcgcgc agcgtgccgt cgagttcagc gttccccatc
     6061 ggctcgcgcc aacctgacct agcgcg
//
LOCUS       DAOORV010000191         6078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15785_length_6078_cov_5.248547, whole genome shotgun sequence.
ACCESSION   DAOORV010000191 DAOORV010000000
VERSION     DAOORV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15785_length_6078_cov_5.248547"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="folP"
                     /locus_tag="PKV56_16660"
     CDS             complement(<1..640)
                     /gene="folP"
                     /locus_tag="PKV56_16660"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOX69492.1"
                     /translation="MFWQTSNHRIDLSWPRVMGIVNATPDSFSDGGQFADARAALDHC
                     KRLIDEGADILDIGGESSRPGAAPVSLEQERRRVLPVLEAALALGRPVSIDTTKAELM
                     AQALALGVDIINDINALNAPGAIDAVAAHRSCGVCLMHMQGDPQTMQARAIYADVVSQ
                     VREVLAARVETLRERGIAADRIVLDPGIGFGKTVEQNFELLRRQRELLELGHP"
     gene            complement(725..2626)
                     /gene="ftsH"
                     /locus_tag="PKV56_16665"
     CDS             complement(725..2626)
                     /gene="ftsH"
                     /locus_tag="PKV56_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOX69493.1"
                     /translation="MNNQWFSKIAVWLVIALVLFTVFKQFDRGASSAGQIGYSDFLEE
                     VRSKRIRNVTLQESPAGTEIVATTTDDKRLRSTATYLDRGLVGDLINNGVKFDVKPRE
                     EPSLLMNILVSWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSKARMLDEANNTTTFA
                     DVAGCDEAKEEVKELVDFLKDPQRFQKLGGRIPRGVLLVGPPGTGKTLLAKAIAGEAK
                     VPFFSISGSDFVEMFVGVGAARVRDMFEQAKKSAPCIIFVDEIDAVGRHRGAGLGGGN
                     DEREQTLNQMLVEMDGFETNLGVIVMAATNRPDILDPALLRPGRFDRQVYVTLPDVRG
                     REQILNVHMRKVPVGQDIRADILARGTPGFSGADLANLVNEAALFAARRAGRVVEMMD
                     FEKAKDKIMMGPERKSMIMPEEERKNTAYHESGHALVARLMPKTDPVHKVTVIPRGRA
                     LGVTMQLPEGDRYSLDKERMLSTISVLFGGRIAEEVFMNQMTTGASNDFERATSIARD
                     MVTRYGMTDELGPMVYAENEGEVFLGRSVTKTTSMSEETMRKVDGVIRRIIDEQYAIA
                     RKHIEDNKEKMHVMAQALLEWETIDSDQIDDIMNGKPPRPPKDWTPSSSRNSGPSTPV
                     SADGAPAAA"
     gene            complement(2761..3477)
                     /locus_tag="PKV56_16670"
     CDS             complement(2761..3477)
                     /locus_tag="PKV56_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOX69494.1"
                     /translation="MKVKSKSKKINKAWLLDHLNDTYVKLSKKEGYRARAAYKLKEID
                     ETLGLLKPGQVVVDLGASPGAWSQYVRRKLSIRQADAVGAGLGPLNGTIIALDLLDFE
                     PIDGVVFIQGDFREDSVLAQLETAVSGRPVDVVVSDLAPNLTGIASSDAARVTHLIEL
                     AVEFATRHLTPGGALVCKVFNGGNYSELVGLFKSTFRTVKSIKPKASRDRSSETFLVG
                     MGLKDVHKVVEDSVKRPSQA"
     gene            3498..3971
                     /locus_tag="PKV56_16675"
     CDS             3498..3971
                     /locus_tag="PKV56_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOX69495.1"
                     /translation="MPAITLTSHQRKEKRADAHHLDPVVLIGGDGLTPAVIREIDAAL
                     NAHGLIKVRVFSDERTSREAMLTTLTDQLGAAAIQHIGKLLVLWRPVPPKEKDEREDR
                     MPGPRVVKLLKFSKSGNHRPQVKKIKVLGNERIAAGGEIKRARKRVTSIKRKAVD"
     gene            complement(4018..4455)
                     /locus_tag="PKV56_16680"
     CDS             complement(4018..4455)
                     /locus_tag="PKV56_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HOX69496.1"
                     /translation="MLRRWAGWVSGVWGGLLLGVGFIGAPAGFAVTTAEVAGRSAGRM
                     FAVEAYVSLAVAVIVLILTRRSAGVEPDQPVAPALNTDVLLVLGTLFCTVAGYFVIQP
                     MMAAARAGQGGGLSFGALHGLSAGFFALKALLVLLLAWRLAAR"
     gene            complement(4470..4946)
                     /gene="greA"
                     /locus_tag="PKV56_16685"
     CDS             complement(4470..4946)
                     /gene="greA"
                     /locus_tag="PKV56_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOX69497.1"
                     /translation="MQTIPLTQRGVDKLKDELQRLKSVERHAVIQAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIESKLAAAKVIDPSTLDAEGKVVFGATVDLEDESSGSS
                     VTYQIVGDDEADLKLGLISISSPIARALIGKEVGDIAEVQAPGGVKSYEVMAVRYV"
     gene            complement(5033..>6078)
                     /gene="carB"
                     /locus_tag="PKV56_16690"
     CDS             complement(5033..>6078)
                     /gene="carB"
                     /locus_tag="PKV56_16690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857147.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOX69498.1"
                     /translation="AVKVSEKSPVLLDRFLDDAIEVDVDCIADGSDAESGVMIGGIME
                     HVEQAGIHSGDSACSLPPYSLPVALQDEMRRQTTLMARALKVKGLMNVQFAIQGEGAD
                     AVVYVLEVNPRASRTVPFVSKATGLQLAKIAARCMVGRKLAEQQQKNGKVPAEVIPPY
                     FSVKEAVFPFNKFPGVDPVLGPEMRSTGEVMGVGRSFGEAMLKSQLGAGSRLPNKGTV
                     CITVKNSDKQRAVVVARDLHALGFQIVATKGTAAAISDGGVPVRAVNKVKDGRPHIVD
                     MVKAGDIQLVFTTVDETRTAIADSRHIRQAALANRITYYTSMAGCEAAVEGMKHQDDL
                     VVLSLQELHAELH"
BASE COUNT         1163 a         1986 c         1791 g         1138 t
ORIGIN      
        1 gcggatgacc gagctcaagc agttcacgct ggcgccgcaa caactcgaaa ttttgttcga
       61 ccgtcttgcc gaatccgatg cccggatcga gcacgatgcg atcggctgca atgccgcgct
      121 cgcgcagcgt ttcaacccgc gcagccagca cctcgcggac ctgcgacacc acgtccgcat
      181 agatcgcgcg agcctgcatc gtttgcgggt ctccctgcat gtgcatcagg cagacaccgc
      241 agcttcgatg cgcagcgacg gcgtcgatcg ctccaggcgc gttcaacgca ttgatgtcat
      301 tgatgatgtc gacaccgagg gccaaggctt gagccatcaa ctccgccttg gtcgtatcga
      361 tcgagactgg ccgccccagt gcgagcgcag cctccagcac aggcagcact cggcggcgct
      421 cctgctccag ggacacgggc gctgcgccgg gacgactcga ttcgcccccg atgtcgagga
      481 tgtcggcgcc ctcgtcgatc aggcgcttgc aatgatccag cgccgcgcgc gcatcggcga
      541 actgaccgcc atcggaaaac gagtcgggcg tcgcgttgac gatgcccatc acccgtggcc
      601 agcttaggtc gatgcgatgg ttcgaggttt gccagaacat ggggacgcgc gttgtgccgt
      661 gaaatctgct gggtcggcaa cgaaaacggg gccgaagccc cgtttgagtg tcagacgtcg
      721 agcgtcaggc ggcagcgggc gcaccgtcgg ccgaaaccgg ggtcgatgga ccgctgttgc
      781 gactggatga aggggtccag tccttcggtg gcctgggtgg cttgccgttc atgatgtcgt
      841 cgatctggtc gctgtcgatg gtttcccatt cgagaagcgc ctgagccatc acgtgcatct
      901 tctccttgtt gtcctcgatg tgcttgcgcg caatcgcgta ctgctcatcg atgatgcgcc
      961 gaatcacgcc atcgaccttg cgcatcgtct cttccgacat cgacgtggtc ttggtcaccg
     1021 agcggccgag gaagacctcg ccctcgttct cggcgtagac catcgggccc agctcatcgg
     1081 tcataccgta gcgcgtgacc atgtcacggg caatggatgt ggcgcgctca aaatcgttgc
     1141 tggcaccggt ggtcatctga ttcatgaaca cttcttcggc aatgcgccca ccgaagagca
     1201 ccgaaatggt cgacagcatg cgctccttgt cgaggctgta tcgatcgccc tccggcaact
     1261 gcatggtcac gcccagggca cgaccgcgag gaatcacggt caccttgtga accgggtccg
     1321 tcttgggcat cagacgcgcg accagagcat ggccggattc gtgatacgcg gtgttcttgc
     1381 gctcctcctc cggcatgatc atcgacttgc gctccgggcc catcatgatc ttgtccttgg
     1441 ccttctcgaa gtccatcatc tcgacaacac ggccggcgcg tcgcgcggca aacagcgcgg
     1501 cttcgttgac caggttggcc agatctgccc cggaaaaccc tggcgtgccc cgcgccagaa
     1561 tatcggcacg aatgtcctgg cccaccggca ccttgcgcat gtgcacgttg agaatctgct
     1621 cgcggccacg cacgtcaggc agcgtcacat acacctgccg gtcgaagcga ccgggacgca
     1681 acagggccgg gtcgagaatg tccggccggt tggtggcggc catcacgatc acgcccagat
     1741 tggtctcgaa accgtccatc tcgacgagca tctgattcaa ggtctgctcg cgctcgtcgt
     1801 taccgccgcc caggccagcc ccgcggtgcc gaccgacggc atcgatctcg tcaacgaaga
     1861 tgatgcatgg cgcgctcttc ttggcctgct cgaacatgtc gcgcacacga gcagcgccca
     1921 cgccgacgaa catctcgacg aagtccgaac ccgaaatgct gaagaacggc accttggcct
     1981 cgccagcaat ggccttggcc agcaaggtct tgccagttcc tggggggccg accaggagca
     2041 cgccgcgcgg gattctgccg cccaacttct ggaatcgctg cggatccttc aggaaatcga
     2101 ccagttcctt gacctcttcc ttcgcctcgt cgcaacctgc gacgtcagca aacgtggtcg
     2161 tgttgttggc ctcgtcgagc atgcgagcct tcgacttccc gaagctgaac gcgccgccct
     2221 tgccgccgcc ttgcatctgg cgcatgaagt agatccagac gccgatcagc agaagcatcg
     2281 gtccccagga cacgaggatg ttcatcagca acgagggttc ttcgcgcggc ttgacatcga
     2341 acttgacgcc gttgttgata aggtcgccga ccagaccacg atccaggtac gtcgcggtgc
     2401 tgcgcagtcg cttgtcgtcc gtcgtcgtcg cgacgatttc cgtgcctgcc gggctttcct
     2461 gcaacgtgac gttgcgaatg cgcttgctgc gtacttcttc gagaaaatcg gagtacccga
     2521 tttggcctgc agaagaagcg cctcgatcaa attgcttgaa aacggtgaaa agcacgagtg
     2581 caatcaccaa ccagacggca attttcgaga accattgatt gttcaccgag gctccaattt
     2641 ttcaacttca gacgtcggcg tgcaaatctg gacccgacca agttctctgt atatagaggc
     2701 gagtttatgc gactcaagtg aggcgaggcc tttgaagcct ccactccggt cgcaggtgtg
     2761 tcacgcctgc gacggtctct tcacggagtc ttcgaccact ttgtgcacgt ccttgagacc
     2821 cattccgacc aggaaggttt cagaagatcg atcgcgagag gctttcggct tgattgattt
     2881 cacagtgcga aaagtcgact tgaacaagcc gacgagttca ctgtagttgc cgccgttgaa
     2941 aaccttgcaa accagtgcgc ctcccggagt caggtgccgg gtcgcgaact cgaccgccag
     3001 ttcgatgagg tgtgtcacac gggccgcatc cgaactcgca atgcccgtca gattgggagc
     3061 cagatcggaa accacgacgt cgactgggcg accgctgacg gccgtctcga gctgtgccaa
     3121 gacggaatcc tcccggaaat ccccctggat gaagaccacg ccgtcgatgg gttcgaagtc
     3181 cagcaggtca agcgcgatga tcgtgccgtt caacggcccc aaccctgctc ccaccgcgtc
     3241 ggcctgcctg atagacaact tgcgacgcac atactggctc caggcaccgg gcgatgcacc
     3301 caagtcgacg acgacctgcc ccggcttcaa gaggccgaga gtctcgtcga tctccttgag
     3361 cttgtaggcc gctcgggcgc ggtagccctc cttcttggac aacttgacat aggtgtcgtt
     3421 gaggtgatcg agcagccacg ccttgttgat cttcttgctt ttggatttga ctttcatgct
     3481 gcaccgataa taggcggatg cccgccatca ccctcacatc ccatcagcgc aaggaaaagc
     3541 gcgccgacgc ccaccatctc gacccggtgg tgttgatcgg aggcgacggg ctgacccccg
     3601 ccgtcattcg cgaaatagac gccgcgctga acgcacatgg gctcatcaag gtccgcgtct
     3661 tttcagacga acgaacctcg cgtgaagcca tgctcaccac actgaccgat caactcggcg
     3721 ccgctgcgat ccagcacatc ggcaagctgc tcgtcctgtg gcgcccggtt cccccgaagg
     3781 aaaaggacga gcgcgaagac cggatgccgg ggcccagggt cgtcaagttg ctgaaattct
     3841 ccaagagcgg aaatcaccgc ccccaggtga agaagatcaa ggttctcggc aatgagcgca
     3901 ttgcggcagg cggcgagatc aagcgcgccc gcaaacgcgt gaccagcatc aagcgcaagg
     3961 cagtcgactg actttggagc ggatccggct ggcgcgaaag tgcagcagcc gggccgctca
     4021 acgagctgcc agccgccagg ccagcaaaag caccagcagt gccttcagtg caaagaatcc
     4081 tgcagacagg ccgtgcagcg ctccgaatga caggccacct ccctgcccgg cacgggcggc
     4141 ggccatcatc ggctggatca cgaagtaccc ggcaacggtg cagaacagcg tgccgagaac
     4201 cagcagcaca tcggtattga gagcaggggc cacgggctgg tccggctcaa cgcctgccga
     4261 cctgcgcgtc aggatcaaca cgatcaccgc gactgccagg ctcacataag cctcgaccgc
     4321 aaacatgcgg ccggcactgc ggccggcgac ctcggcggtt gtcacggcga atccggctgg
     4381 cgcgcctatg aaccccacac ccaggagcag gccaccccat actcccgaca cccagccagc
     4441 ccagcggcgc agcatcgtca cgtgtctgtt caaacgtaac ggacggccat gacctcgtag
     4501 ctcttgacac cgccgggcgc ctgcacctcg gcgatgtcgc cgacctcctt gccgatgagc
     4561 gctcgggcaa tcggcgaact gatggagatc aggcccagct tcaggtcggc ctcgtcatct
     4621 ccaacgatct ggtaggtgac cgacgaaccg ctggattcgt cttccagatc gacagtggca
     4681 ccgaagacga ccttgccttc ggcatccagt gtcgacggat cgatcacctt ggcggccgca
     4741 agcttgctct cgatctccag gatgcgccct tcgatgaagc cctgcttgtc cttcgcagcc
     4801 tcgtactcgg cgttctcgga cagatcgccc tgcgccctcg cctcggcaat cgcctggatg
     4861 acggcatggc gctccacgct cttcagacgt tgaagttcat ccttgagctt gtcgacaccg
     4921 cgttgggtga gaggaatggt ctgcatatgg gtaacccggg acaaaaatga agccgccgcc
     4981 ggaagctcca gcggcggctg taggggatga attagcgcgc tatcggatga gtttagtgca
     5041 actcggcatg cagttcctgc agcgaaagca ccaccagatc atcctgatgt ttcatgccct
     5101 cgaccgccgc ttcgcatccc gccatgctgg tgtagtaggt gatccggttc gcgagggccg
     5161 cctggcggat gtggcgcgaa tcagcgatcg ccgtgcgggt ttcatcgaca gtcgtgaaga
     5221 ccagctggat gtctccggcc ttcaccatgt cgacgatgtg cggccggcca tccttcacct
     5281 tgttgaccgc cctgacgggc accccgccgt ctgagatcgc ggcggccgtg cccttggtcg
     5341 caacgatctg gaagccgagc gcgtgcagat cacgggcgac gaccacggcc cgctgcttgt
     5401 cgctgttctt gacggtgatg cacaccgtgc ccttgttcgg caaccgcgaa cccgcgccaa
     5461 gctggctctt cagcatcgct tcgccgaagc tgcggccgac acccatcacc tcgcccgtgg
     5521 agcgcatttc cggcccaagc accggatcga cacccgggaa cttgttgaac gggaacacag
     5581 cttccttgac gctgaaatac ggcgggatga cttcggcagg caccttgccg ttcttctgct
     5641 gctgctccgc cagcttgcgg ccgaccatgc agcgcgcagc gatcttcgcc agttggagtc
     5701 cggtggcctt cgacacgaac gggaccgtgc gcgatgcgcg cggattcacc tcgagcacgt
     5761 agacgacggc atcggccccc tcgccctgga tggcaaactg cacgttcatc aggcccttga
     5821 ccttcagcgc acgcgccatc agcgtggtct ggcggcgcat ctcgtcctgc agcgccacgg
     5881 gcagcgagta aggcggcagc gaacaggccg agtcgccgga gtggatgccg gcctgctcga
     5941 cgtgttccat gatgccgccg atcatcacgc ccgactcggc atcgctgccg tcggcgatgc
     6001 agtcgacatc gacctcgatc gcatcgtcga ggaagcggtc cagcagcacc ggcgacttct
     6061 cgctgacctt gacggctt
//
LOCUS       DAOORV010000192         6068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15825_length_6068_cov_11.212539, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000192 DAOORV010000000
VERSION     DAOORV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15825_length_6068_cov_11.212539"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..2329)
                     /gene="pglX"
                     /locus_tag="PKV56_16695"
     CDS             complement(23..2329)
                     /gene="pglX"
                     /locus_tag="PKV56_16695"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HOX69499.1"
                     /translation="MINAEQLLVDLTRQLKGLESDLRQRIAEQPGLNDSLRTEWQAAL
                     NAKRCAETYLTWADQVITQAGVHWLLSCVFLRFIEDNLLVDRPWLAGTPESGRLALAR
                     DRHEDYFSTHPLESDRDYLLSAFREAGALPGLHTFFDEAHNPVFRLGISGDAAMALRQ
                     FWQQVNTDSGLLLRDFTDPAWNTRFLGDLYQDLSEATRKRYALLQTPEFVEEFILDRT
                     LTPAIRTFGFREVRMIDPTCGSGHFLLGGFARLVNEWQSNEPARNPRDIAQKALDAVA
                     GVDLNPFAVAISRFRLLVAALRVAGTRRLAEAPDFRTHVAIGDSLLHGMRFGLTATQD
                     LLSHDQYEDTGLAHAYASEDLIQVQQILSQQYHAVVGNPPYIVVEDARLNVAYRNRYA
                     SCQGQYSLGVPFTERFFDLAVRGQGSNSGFVGLITANSFMKREFGPRLVTEVLPRFDL
                     THVVDTSGAYIPGHGTPTVILFGRSRLPVADQVRAVLGIKGEPSAPDDPAQGKVWLSI
                     LEAIDLPGFENRFISTTDYPRAKFFQRHPWRIRGGGAAELADWLDEQHRGRLQRGSVV
                     SGTGRTSADEAFVVPPQLPQRNALEARVFGVLVEGDMLRDWSLSTGDAVFAPYSDSGL
                     EPIDAVPRASRWLWPMRTSLGARVTFGRSTYAEEGRPWWGWHQVTVERLRYRHIIACA
                     FVATHNHFVLDRGGKVFKQTAPAILLPAHQTEDHYLGLLGLLNSSTACFWMKQTFFDR
                     GNGGIGGGIAAEEWERFYEPARVKVVVA"
     gene            complement(2393..>6068)
                     /gene="pglW"
                     /locus_tag="PKV56_16700"
     CDS             complement(2393..>6068)
                     /gene="pglW"
                     /locus_tag="PKV56_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system serine/threonine kinase PglW"
                     /protein_id="HOX69500.1"
                     /translation="GIRPSNKHRQVGDYKLGKLLAEGEGFQDWEAQHVTIETVHRRVR
                     IYTVATASNPEARANRVRQARREFEVLEGIDHPGILKVKDYKETELGPALVFDHDPKA
                     VRLDHLLRDHGTRLTVTQRLQLVRDIAETLKYAHSKRLYHRALGPQSILVQNVESSSY
                     QLRLMNWQTASRNAGDSASPDTVHRTTGTRHVEDYVEDPGLVYLAPETTRADPAHGPS
                     LDVFSLGCIAYHVFSGQPPAESVLDLNEKLRIGQGLNLSDVMDGCGAHQQELVHVATC
                     PEVLARYDTIRGFIDELDGVEDELTTPDPEATVDPSVAGKDDRLEGGFTVVKRMGKGS
                     SSDALLVREDGSDEELILKVALDVAHNDRLVAEGEVLAKLHHHNIVAHRRTLSVAGRT
                     ALLLKTAGDRTLAETLKDVREQGRLSLDMLQRFGEELIEAVNHLEAEGVAHRDIKPEN
                     IGITRNRTGKLQLVLFDFSLCRTPTDNITAGTHPYLDPFLSLRKPPRWDLYAERFATA
                     VTLYEMAVGQPPVWGDGQTSPAMLDVEATIESDVFDPVTREGFTAFFAKALKRDYSQR
                     YDNAEDMLRAWRAIFAARQTVHPGEGGAESALATIAMTATPLTTMAELGYSLEAQDVL
                     ERMGIHNARELLAVDRIRFRYLRGVGDKIRKEIRLTAKELARRRPDLTQGRNTAHDAD
                     EDTPGAVSVNELASQLLPRRPAGDDRPEEAALAHYLGLDDAVKPGAWPTVGEAAQASE
                     VDRAVLTTALIKARERWLRNTAFTELRQQLEALVRSQGQVMSANEASLALLSLRGCAE
                     QDDTERLRQASAVLRAAIEAESHLDQPRFEAFDHAPVTLIATAAAWADYARQLGTAAD
                     ACAMADPLLPPARVLEMLEGVSPPAAASTAAGAGSPLSPALSPTRLLRLAASASRKAA
                     LSSRQEIYPRNMPALQALKQSINALVGATLSPSALQTRVRGRYPEATPLPGRPELDRL
                     LDEAGAPLNWDATAADGAGAYRSATLGSTATAGTTTQFSRQGTMLSTGAGPDGTVVDA
                     AAAEDRLQRSLRQGGLLVITVDPRLARRAETELLHRFSQPKGTGHKLQRMNLDTVLLN
                     ELKAQATAARVDWNVVLHADAADKASRDWTNLIRLVQRTLPALRTALLQSPAPLLLVS
                     AGLLARYDLMPLITEIEAATGRPGHTPSAWLLLPTAHQGLPVIDGVAVPLVNNINNTQ
                     ALALPQAWVENQHRAGLIHG"
BASE COUNT          949 a         1843 c         2131 g         1145 t
ORIGIN      
        1 actcagcaga tggctgcgtg agtcaggcga caactacctt gacgcgcgcc ggttcgtaaa
       61 agcgttccca ctcctctgcg gcgatccctc cgccaatacc gccgtttccg cgatcaaaaa
      121 aggtttgctt catccaaaag caagccgtag atgaattgag cagcccgagt aggccaagat
      181 agtgatcttc tgtctgatgg gcaggaagaa gaatcgcagg cgctgtctgc ttgaatactt
      241 tgccgccacg gtcaagcacg aaatggttat gcgtagcaac aaaagcgcag gcgatgatgt
      301 gccgatatct cagtcgctcg actgtgacct gatgccaacc ccaccatgga cgcccctcct
      361 cggcgtacgt gcttctgccg aacgtaactc tcgcgccaag tgatgtacgc atcggccaca
      421 gccagcgtga cgcacgcggg acggcatcta tcggctctag acctgagtca ctgtaaggtg
      481 cgaagacagc gtctccggtt gacaagctcc aatcgcgcag catgtcgcct tcgactaaga
      541 cgccgaagac tcttgcctca agcgcattgc gttgcgggag ttgaggtgga actacaaacg
      601 cctcgtctgc gctagttcga cctgtccccg agacaactga tccacgctgc aatcggcctc
      661 gatgttgctc atctagccaa tctgcaagct cggctgcccc accacctcga attctccaag
      721 gatgcctctg aaagaacttg gctcttggat agtcagtggt gctaatgaat cgattctcga
      781 accctggcaa gtcgatagct tccagtatgg agagccacac ttttccctgt gcggggtcgt
      841 ccggcgcaga cggctcgccc ttgatcccaa gtaccgcacg cacttgatct gcgaccggta
      901 gacgactgcg tccgaaaagg atcacagtcg gtgttccgtg gcctggaatg taggcgccag
      961 atgtgtcaac aacatgggtc aagtcgaagc gaggcaggac ctcagtcaca agacgagggc
     1021 cgaactcacg cttcataaac gaattggccg tgatgaggcc tacgaatcca gagttcgacc
     1081 cttggccacg gactgccaga tcgaagaacc tttcggtaaa cggcacgccc agtgaatact
     1141 gtccctgaca acttgcatag cgattcctgt aggcgacgtt tagcctcgcg tcttcaacga
     1201 cgatatacgg cgggttaccg actacggcgt ggtactgctg gctgaggatc tgctgcacct
     1261 gaatcagatc ttcgcttgcg taggcgtggg ctaggccggt gtcctcgtat tggtcgtggc
     1321 tcaacagatc ttgcgtggcg gttaagccga agcgcatgcc gtgcagcagg ctgtcgccaa
     1381 tggccacatg ggtgcgaaag tcgggcgctt cagccaggcg tcgcgtgccg gccacgcgca
     1441 gcgccgcgac caggaggcga aagcggctga tcgcaacagc gaaggggttt aggtcgacgc
     1501 cggccacggc gtccagcgct ttctgtgcaa tgtcgcgcgg gttgcgtgcg ggctcattgc
     1561 tctgccactc attcaccagc cgcgcaaagc cgcccagcag aaagtggcct gagccgcagg
     1621 tggggtcgat catccgcact tcgcggaagc cgaaggtacg aattgccggc gtaagcgtgc
     1681 ggtcgaggat gaattcttcg acgaactccg gcgtctgcag cagcgcgtag cgcttgcggg
     1741 tggcctcgct caggtcctgg tacaggtcgc ccaggaagcg ggtgttccag gccgggtcgg
     1801 tgaagtcgcg cagaagcagg ccactgtcgg tgttcacctg ctgccagaac tggcgcagcg
     1861 ccatcgccgc gtcgccgctg atgccgagac ggaagacggg gttgtgcgct tcgtcgaaga
     1921 aggtgtgcag gccgggcaga gcgccggctt cgcgaaacgc cgacagcaga tagtcgcggt
     1981 cgctttccag cgggtgcgtg ctgaagtagt cctcgtgccg gtcgcgggct aaggctaggc
     2041 ggcctgactc gggcgtgccg gccagccagg gccgatcgac cagcaggttg tcttcgatga
     2101 agcgcaggaa cacgcagctc agcagccagt gcacgccggc ttgggtgatg acctggtcgg
     2161 cccaggtcag ataagtttca gcgcaacgct tggcgttgag cgcggcttgc cactcggtgc
     2221 gcaggctgtc gttcaagccg ggttgctcgg cgatgcgctg gcgcaggtcg gattccaggc
     2281 ctttgagctg gcgcgtcagg tcgacgagca gttgttcggc attgatcact tgtcttgttt
     2341 ccctggtgtt cttgttctgc gtgctcaggc cgcgctgcag ccgacgtagc gctcacccat
     2401 ggatgagccc cgcacggtgt tggttctcca cccaggcctg cggcaaggcc agcgcttggg
     2461 tgttgttgat gttgtttacc agcggcacgg ccacgccgtc gatgacgggc aggccctggt
     2521 gtgcggtggg cagcagcagc caggcgctgg gcgtgtgccc ggggcggcca gtggcggctt
     2581 cgatctcggt gatcagcggc atcaggtcat agcgagccaa caggccggcg ctgaccagca
     2641 gcaggggtgc ggggctttgc agcagcgcgg tgcgcagcgc gggcagcgtc cgctgcacca
     2701 ggcgaatcag gttggtccag tcgcggctgg ccttgtccgc ggcgtcggca tgcagcacca
     2761 cgttccagtc cacacgggcg gcggtggcct gggccttcaa ctcgttcagc agcaccgtgt
     2821 cgagattcat gcgttgcagc ttgtggccgg tgcccttggg ctggctgaag cggtgcagca
     2881 gttcggtctc ggcgcggcgg gccagtcgcg ggtccacggt gatgacgagc aggccgccct
     2941 ggcgcaggct gcgctgcagc cggtcttcgg ccgcagcggc gtcgaccacc gtgccgtcgg
     3001 gcccggcgcc ggtgctcagc atcgtgcctt ggcgcgagaa ctgcgtggtg gtgccggccg
     3061 tggccgtgct gcccaaggtg gcggagcggt aggcgccggc accgtcggcg gccgtagcgt
     3121 cccagttcag cggggcgcca gcctcgtcga gcaggcggtc tagctcgggt cggccgggca
     3181 ggggcgtggc ctcggggtag cggccacgca cgcgggtttg cagcgcgctg gggctgagcg
     3241 tggcgccgac caaggcgttg atggactgct tcagtgcctg cagtgcaggc atgttccggg
     3301 ggtaaatctc ttgccggctg gacagcgctg ccttgcgcga ggcagatgcg gccaggcgca
     3361 gcagacgtgt cggtgagagt gcgggcgaga gtggcgaccc tgcgcccgct gcggtgcttg
     3421 ccgcagcagg cggcgaaacg ccttccagca tctcaagtac ccgtgccggc ggaagcaggg
     3481 ggtcggccat tgcgcaggcg tcggccgcag tgcccagttg gcgggcgtag tcggcccagg
     3541 cggcagcagt ggcgatgagg gtgaccggcg cgtggtcgaa ggcttcgaaa cgtggttggt
     3601 ccaggtgcga ctcggcctcg atggccgcac gcagcacggc gctggcctgg cgcaagcgct
     3661 cggtgtcgtc ttgctcggca cagccgcgca gcgacagcaa ggccagcgat gcctcgttcg
     3721 cgctcatcac ctggccctgg ctgcgcacca gggcttcgag ctgctgtcgc aattcagtga
     3781 acgcggtgtt gcgcagccag cgttcgcggg ccttgatgag tgcggtggtg agcacggcac
     3841 ggtccacctc gctggcctgc gcggcttcgc ccaccgtggg ccaggcgccg ggcttgaccg
     3901 cgtcgtccag gcccaggtag tgggcgaggg cggcttcttc gggtcggtca tcgccggccg
     3961 gccgacgcgg cagcagttgc gaggccagtt cgttgacgct gacggcgccg ggcgtgtctt
     4021 cgtcggcgtc gtgtgcggtg ttgcggccct gcgtaaggtc gggccggcgc cgtgccagct
     4081 ccttggcggt gaggcgaatc tccttgcgaa tcttgtcgcc cacgccgcgc aggtagcgga
     4141 agcggatgcg gtcgacggcc agcagctcgc gggcgttgtg gatgcccatg cgctccagca
     4201 cgtcctgcgc ttctaggctg tagcccagct cggccatggt ggtgagcggc gtcgccgtca
     4261 tggcgatggt cgccagggcg ctctcggcgc cgccctcgcc cgggtgcacg gtctggcggg
     4321 ccgcgaagat ggcacgccag gcacgcagca tgtcttcggc gttgtcgtag cgctggctgt
     4381 agtcgcgctt cagggcctta gcgaaaaacg cggtgaagcc ttcgcgcgta acggggtcga
     4441 acacgtcgct ctcgatcgtg gcttccacgt ccagcatggc gggcgatgtc tgcccgtcgc
     4501 cccacacggg cggctggccc acggccatct catagagcgt gacggcggtg gcgaagcgct
     4561 cggcgtagag gtcccaccgc ggcggcttgc gcagtgagag gaacgggtcg aggtaggggt
     4621 gggtgccggc ggtgatgttg tcggtgggcg tgcggcacag cgagaagtcg aacagcacga
     4681 gctgtagctt gccggtgcga ttgcgcgtga tgccgatgtt ctcgggcttg atgtcgcggt
     4741 gggccacgcc ttctgcttcg aggtggttca ccgcctcgat gagttcttca ccgaagcgct
     4801 gcagcatgtc cagcgacagt cgtccctgct cgcgcacgtc cttcagcgtc tcggccagcg
     4861 tcctgtcgcc ggctgtcttc aacagcaacg cggtgcggcc tgccacgctg agcgtgcggc
     4921 ggtgggccac gatgttgtgg tggtgcagct tggccagcac ttcgccttcg gcgaccaggc
     4981 ggtcgttgtg ggccacgtcc agagcgacct tgaggatcag ctcttcgtcg ctgccgtctt
     5041 cgcgcaccag cagcgcgtcg ctggacgagc ccttgcccat gcgcttgacg acggtgaagc
     5101 cgccttcgag ccgatcgtcc ttgccggcaa cgctggggtc gacggtggct tcggggtcgg
     5161 gcgtggtgag ttcgtcttcg acaccatcga gttcgtcgat gaagccgcga atggtgtcgt
     5221 agcgtgccag cacctctggg caggtggcga cgtgcacgag ttcctgctga tgtgcgccgc
     5281 agccgtccat cacgtcggac aggttcaggc cctggccaat gcgcaacttc tcgttcaggt
     5341 ccagcaccga ttcggcaggg ggctggccgc tgaacacgtg gtaagcgatg cagccgagcg
     5401 agaacacatc aaggctgggg ccgtgcgcgg ggtcggcgcg ggtggtttcg ggcgcgaggt
     5461 agaccaggcc cgggtcttcg acgtagtcct ccacatgccg cgtgccggtg gtgcggtgca
     5521 cggtgtcggg cgacgccgaa tcgcctgcgt tgcgcgacgc ggtctgccag ttcatcagcc
     5581 gcagctggta gctgctgctc tcgacgttct ggaccaggat gctttgcggc ccgagcgcgc
     5641 ggtggtacag gcgcttggag tgcgcgtact tcagcgtctc ggcaatgtct cgcacgagct
     5701 gcaggcgctg cgtgacggtg aggcgcgttc cgtggtcacg aagcaggtgg tccaggcgca
     5761 cggctttggg gtcgtggtcg aagaccaacg ccgggcccag ctcggtttct ttgtagtcct
     5821 tgaccttcag gatgccgggg tggtcgatgc cttcgagcac ctcgaattca cggcgcgcct
     5881 ggcgcacgcg gttggcgcgg gcctcggggt tggaggccgt ggccacggtg tagatgcgca
     5941 cgcggcggtg caccgtttcg atggtgacgt gttgggcttc ccagtcctgg aagccttcgc
     6001 cttcggcgag caacttgccg agcttgtagt cgccgacctg gcggtgcttg ttggacgggc
     6061 ggatgccg
//
LOCUS       DAOORV010000193         6055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_15873_length_6055_cov_5.991167, whole genome shotgun sequence.
ACCESSION   DAOORV010000193 DAOORV010000000
VERSION     DAOORV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15873_length_6055_cov_5.991167"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="pnp"
                     /locus_tag="PKV56_16705"
     CDS             complement(<1..1456)
                     /gene="pnp"
                     /locus_tag="PKV56_16705"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOX69501.1"
                     /translation="MSMFNKVTKTFQWGQHTVVLETGEIARQSGGAVLVNIDDTVVLA
                     TVVGAKNPKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREGRPSELETLTSRLIDRPLR
                     PLFPEGFYNEVQVVVHVMSLNPEVSADIAALIGSSAALAISGIPFDGPIGAARVGYVN
                     GEYVLNPGKTQLLDSAMDLVVAGTQAAVLMVESEAKQLSEEIMLGGVVFGHEQSKIAI
                     AAINELVRDAGKPAWDWKPASKDEPFIAKVVELAEGPLRTAYQIRSKQARTQATREVT
                     AKTMAALTADGASIDKVKVDNVLFDIEAKIVRSQILSGEPRIDGRDTRTVRQIEIRNS
                     VLPRVHGSALFTRGETQALVAATLGTDRDSQKIDALAGEYSEHFMLHYNMPPFATGET
                     GRVGTPKRREIGHGRLAKRALVAVLPDRSDFPYSMRVVSEITESNGSSSMASVCGGCL
                     ALMDAGVPLKAHVAGIAMGLIKDGNRFAVLTDILG"
     gene            complement(1656..1928)
                     /gene="rpsO"
                     /locus_tag="PKV56_16710"
     CDS             complement(1656..1928)
                     /gene="rpsO"
                     /locus_tag="PKV56_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOX69502.1"
                     /translation="MMTSTVAQKAEIVKANARSANDTGSPEVQVALLTARINELTPHF
                     KTHLKDHHGRRGLLKMVNTRKSLLAYLKDRDADRYTALIQKLGLRK"
     gene            complement(2064..3095)
                     /gene="tsaD"
                     /locus_tag="PKV56_16715"
     CDS             complement(2064..3095)
                     /gene="tsaD"
                     /locus_tag="PKV56_16715"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOX69503.1"
                     /translation="MIFLGIESSCDETGVALVEADVEGIPRLRGQALYSQIDMHRAYG
                     GVVPELASRDHIRRVLPLARQVMREARAELRDIDIVAYTQGPGLAGALLVGAGVACAL
                     GASLDRPVVGVHHLEGHLLSPFLGADPPAFPFVALLVSGGHTQLMRVDDVGSYTLLGE
                     TIDDAAGEAFDKSAKLLGLGYPGGPALAQLAVSGDPARFDLPRPMLRSGDLSFSFAGL
                     KTAVMVTLRKLGPSPSDQDRADLAASTQAAIVDVLVRKSITALQDSGLDRLVVAGGVG
                     ANAALRGRLNAEGARRRFNVHYPELALCTDNGAMIALAAAMRWQRRIAAPRRDHDFNV
                     RPRWPLAAA"
     gene            3176..3805
                     /locus_tag="PKV56_16720"
     CDS             3176..3805
                     /locus_tag="PKV56_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOX69504.1"
                     /translation="MKPSLRIVIVAPDTVAAEDPTEDNAEAQAERSRSLRIGLLENGY
                     NIVAVLPADVFLPERLAQISPDMVIVDAESGARDTLEHVVVATRDARRPIVLFTNDED
                     TSHVGAAIAAGVTAYVVSGLAADRVKPVLEIALARFEHEESMRRELADARSQLSDRKV
                     IDRAKGILMKRQGLPENEAYALLRKAAMDKGLKLVEVAQRIIDVAELLA"
     gene            4139..5392
                     /locus_tag="PKV56_16725"
     CDS             4139..5392
                     /locus_tag="PKV56_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX69505.1"
                     /translation="MNLPLTRKIDMSRRTLIKAAAATSAAGLVPGLQSAVYAAGSDKP
                     EKEEVRIGFIPLTDCASVVMASVLGFDKKYGIKIIPTKEASWAGVRDKLVNGELDMAH
                     VLWGLIYGVHLGTSGPKKDMAILMNLNHNGQAITLSKKLSDKGAVDGASLAKLMATDK
                     REYTFAQTFPTGTHAMWLYYWMATYGINPIKDAKVITVPPPQMVANMRVGNMDGYCVG
                     EPWGHRAIADGIGITAITTQDIWKDHPEKALGTTAEFVQKYPNTARAVVMAILEASQW
                     IDAGLKNKNQMAETVADKSYVNTSVDVINQRILGRYQNGLGKTWDDPNHMKFFNDGVV
                     NFPYLSDGMWFLTQHKRWGLIKDHPDYLGVAKQINQIDLYKQAASALKISIPKDPMRS
                     SKMVDGVVWDGKDPKKYADSFAVKA"
     gene            5428..>6055
                     /locus_tag="PKV56_16730"
     CDS             5428..>6055
                     /locus_tag="PKV56_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX69506.1"
                     /translation="MVSAVFHSPLAASAELQAQKLAHEKTPATAGVTAAPQAPAASQR
                     TAFDWGAFWLKVLSPLAGMGLLVGLWALATMNSTTFPSPTETFKAAVELFADPFYRKG
                     PNDQGIGWNILFSLERVAIGFGLAAAVGIPMGFMIGRFKVLNDMLSPLISLLRPVSPL
                     AWLPIGLLVFKSANPAAVWTIFICSIWPMIINTAVGVQRVPQDYLNVAR"
BASE COUNT         1138 a         1938 c         1920 g         1059 t
ORIGIN      
        1 cacccaggat gtcggtcagc accgcgaagc ggttgccgtc cttgatcagg cccatcgcga
       61 tgccggccac atgtgccttc agtggcacgc ccgcgtccat cagagccagg caaccgccgc
      121 agaccgaggc catcgacgac gagccattcg actcggtgat ttccgagacg acccgcatgg
      181 aatacgggaa gtcagagcga tccggcagca ccgcgaccag cgcgcgcttg gccaggcggc
      241 cgtggccgat ctcgcggcgc ttcggcgtgc cgacgcgacc cgtctcgccg gtggcgaacg
      301 gaggcatgtt gtagtgcagc atgaagtgct cgctgtactc gccggccaac gcgtcgatct
      361 tctgggaatc gcggtcggta ccgagcgtgg cagccaccag cgcctgcgtt tcgccgcgcg
      421 tgaacagagc cgaaccgtga acccgcggca gcacgctgtt gcggatctcg atctggcgca
      481 cggtgcgcgt gtcccgaccg tcgatgcgcg gctcgcccga gaggatctgg ctgcgcacga
      541 tcttggcttc aatgtcgaac aggacgttgt cgaccttgac cttgtcgatc gaggcgccat
      601 cggcggtcag cgctgccatc gtcttggccg tgacctcgcg cgtggcttgc gtgcgagcct
      661 gcttcgagcg gatctggtat gcggtgcgca gcggaccttc ggccagttcg acgaccttcg
      721 cgatgaaggg ctcgtccttc gatgcgggtt tccagtccca ggccggcttg ccggcgtcac
      781 gaaccagttc attgatcgca gcaatcgcga tcttgctctg ctcgtggccg aacaccacgc
      841 cgccaagcat gatttcctcg ctcagttgct tggcttccga ctcgaccatc agcacggctg
      901 cctgggttcc ggcgacgacg agatccatcg ccgaatcgag cagctgggtc ttgcccggat
      961 tgagcacgta ctcgccgttg acgtagccaa cgcgagcagc gccgatcggt ccgtcgaacg
     1021 ggatgccgga gatggccagc gcagcgctcg aaccgatcag tgcggcgatg tcggccgaga
     1081 cttccggatt cagcgacatg acgtggacga cgacctgcac ctcgttgtag aagccttcag
     1141 ggaacagagg acgcagcgga cggtcgatca ggcgcgaggt cagcgtttcg agttcgctag
     1201 ggcggccttc gcgcttgaag aagctgcccg ggatcttgcc ggcagcgtag gtcttctcga
     1261 tgtagtcgac ggtcagcggg aagaaatcct gccctggctt cgggttcttg gcaccgacca
     1321 cggtggcgag caccacggtg tcgtcgatgt tgaccagcac cgcgccgcca gactggcggg
     1381 cgatttcgcc ggtttccagc accacggtgt gctgacccca ttggaaggtc ttggtgactt
     1441 tgttgaacat gctcatgtgt ttctccggtt gagatcagcg ccttgcgggt ggcgccttcg
     1501 aacccagagc tgcggcatcc tggcgcgatg ccattccagc ggcgttcact gtcaaacgcc
     1561 agtggaatga cacatcgtca tcgttgggtg cccggctcaa aacgagaaga gcctgtgctg
     1621 gagacccaga caggctcttt gcttcgatcg gcagattact tgcgcagacc cagcttctgg
     1681 atcagtgcgg tatagcgatc ggcgtcgcgg tctttcaggt aggccagcaa gctcttgcgc
     1741 gtgttgacca tcttcaacag accacggcgg ccgtggtgat ccttcagatg cgtcttgaag
     1801 tgcggcgtca gttcgttgat gcgagcagtc agcaacgcaa cctgaacctc gggagatccg
     1861 gtgtcgttgg cgctgcgcgc gttggccttg acgatttcgg ccttttgggc gacggtggag
     1921 gtcatgatgt ttccttgtga tgaagccgac tcttcgcagt ggatgcgggc ttcatgttgt
     1981 gacttgcggt cgcgggtgaa actacctgcg ccgtgcgaaa accacggact gaacctgtcc
     2041 gaagccgatc gattatagcc cgctcaggca gccgccagtg gccagcgtgg gcggacgttg
     2101 aagtcgtggt cgcggcgggg cgcagcgatg cgtctctgcc accgcatggc agcggccagc
     2161 gcgatcatcg cgccgttgtc ggtgcacagg gccagttcgg ggtaatgcac gttgaaccgc
     2221 cggcgcgcac cctccgcgtt gagccgaccg cgcagcgccg cgttggcgcc gactccacct
     2281 gcgacgacga gccgatcgag cccgctgtcc tgcaaggcgg tgatcgactt gcgcaccagg
     2341 acgtccacga ttgcagcctg ggtgctggca gccagatcgg cccgatcctg atcgctgggc
     2401 gacgggccga gcttgcgcag ggtcaccatg acggctgtct tcagcccggc gaacgagaaa
     2461 ctcagatcgc cgctgcgcag catcggtcga ggcagatcga agcgggccgg gtcgccgctg
     2521 accgcgagct gcgcgagcgc cgggccgccg gggtagccca ggcccagcaa tttcgccgac
     2581 ttgtcgaaag cctcgccggc agcgtcgtcg atcgtttcgc ccagcagcgt gtaggaaccg
     2641 acatcgtcga cgcgcatcag ctgcgtgtgc ccgcccgaca ccagcagcgc aacaaacggg
     2701 aacgcaggcg ggtcggcgcc gaggaacggc gacagcagat gaccttcgag atgatggaca
     2761 cccaccaccg ggcgatcgag ggaagctccc agggcgcagg ccacccccgc accgacgagc
     2821 aacgcgccgg cgaggcccgg accctgggtg taagcgacga tgtcgatgtc gcgcaattca
     2881 gcccgggctt cccgcatcac ctgccgcgca aggggcagca cgcggcggat gtggtcgcgc
     2941 gaggcgagct ccggaaccac accaccgtat gcgcgatgca tgtcgatctg gctgtacagg
     3001 gcctggccgc gcaggcgcgg aatgccctcg acatcagcct cgaccaaagc cacgccagtt
     3061 tcgtcgcaag acgattcgat gcccagaaaa atcatgcagg aagtctaggc gacaggccgg
     3121 gaccggacgc cccgcgcggt acccggtggc gtggaatttg cgtccctata aagccatgaa
     3181 accgtccctg cgtatcgtca tcgtggcacc agacaccgtg gccgccgaag acccgaccga
     3241 ggacaacgcc gaagcccagg ccgaacgttc gcgcagcctg cgcatcggcc tgctcgagaa
     3301 cggctacaac atcgtcgctg tgctgcccgc agacgtgttc cttcccgagc gtctggcaca
     3361 gatctcaccg gacatggtga tcgtcgacgc tgaaagtggc gcgcgcgaca cgctcgagca
     3421 cgtggtcgtc gccactcgcg acgctcggcg gccaatcgtg ctgttcacca acgacgaaga
     3481 caccagccat gtcggtgcag ccatcgccgc tggcgtcacg gcgtatgtgg tgtccggact
     3541 ggctgccgac cgggtcaagc ccgtgctcga gatcgccctc gcccgttttg aacacgagga
     3601 atcgatgcgt cgcgaactcg ccgacgcgcg ctcccagctg tccgatcgca aggtgataga
     3661 tcgagccaag ggcatcctga tgaagcgcca gggattgcct gagaacgaag cctacgcgct
     3721 gctgcgcaag gcggcgatgg acaaggggct gaagctggtg gaagtcgcgc agcgcatcat
     3781 cgacgtcgcc gaactgctgg cttgatccca cctcgggcgc ccgatgccca atccctgtgc
     3841 agcgcggacc cgatcgacag gggttcgcta cacagagcgc acgccagccg catgattttg
     3901 gtgcgccgca gcaacttcag gccgaaaagt gatgccggca cgctggcacg accattgctg
     3961 ctactcacct cagtaggtca acggcgatct acgaatgact gcgttgttca ccaattcggt
     4021 gaattgcgca acggacgacg gcgtccctca ctgtcactga tcttcaacga tcgacagcgt
     4081 gggcgccgtt tttgtttgtg catttgaatt tcatcgcctc gccatttcga aggacaccat
     4141 gaacctccct ctgactcgaa agatcgacat gagccgccga accctgatca aagcggctgc
     4201 cgccaccagc gctgccggac tcgttcccgg actgcagagc gccgtctacg ccgccggctc
     4261 ggacaagccg gaaaaggagg aagtccgcat cggcttcatc ccgctgaccg attgcgcttc
     4321 ggtcgtgatg gcgtcggtgc tcggcttcga caagaagtac ggcatcaaga tcatcccgac
     4381 caaggaggcc tcctgggctg gcgtgcgcga caagctggtc aatggcgagc tcgacatggc
     4441 gcacgtgctg tggggcctga tctacggcgt gcacctcggc accagcggcc cgaagaagga
     4501 catggccatc ctgatgaacc tgaaccacaa cggccaggcc atcacgctgt cgaagaagct
     4561 gtccgacaag ggggcagtcg acggcgcctc actggccaag ctgatggcga ccgacaagcg
     4621 cgagtacacc ttcgcgcaga ccttcccgac aggcacccac gcgatgtggc tgtactactg
     4681 gatggcgacc tacggcatca acccgatcaa ggatgccaag gtcatcaccg tgccaccgcc
     4741 ccagatggtg gcgaacatgc gagtgggcaa catggacggc tactgcgttg gtgagccctg
     4801 ggggcaccgc gccattgccg acggcatcgg catcaccgcg atcaccacgc aggacatctg
     4861 gaaagaccat cccgagaagg cactggggac gaccgccgag ttcgtccaga agtacccgaa
     4921 cacggcgcgt gcagtggtga tggccattct cgaggccagc cagtggatcg atgctggcct
     4981 caagaacaag aaccagatgg ccgagacggt ggctgacaag tcctacgtca acaccagcgt
     5041 cgacgtgatc aaccagcgca tcctcggccg gtaccagaac gggctgggca agacctggga
     5101 cgatccgaac cacatgaagt tcttcaacga cggcgtggtc aatttcccgt acttgagcga
     5161 cggcatgtgg ttcctcaccc agcacaagcg ctggggcctg atcaaggacc atccggacta
     5221 cctcggcgtg gccaagcaga tcaaccagat cgatctgtac aagcaggccg cctcggccct
     5281 gaagatcagc attccaaagg atccgatgcg cagcagcaag atggtcgacg gggtggtctg
     5341 ggatggcaag gatccgaaga aatacgccga cagctttgct gtcaaggcct gatccggccg
     5401 gcccgcatcc accaccagga gtccatcatg gtcagtgcag tgtttcactc tccccttgca
     5461 gcatcggccg agctgcaggc ccagaagctg gcgcacgaga agaccccggc cactgcaggg
     5521 gtcaccgcgg caccacaggc tcccgcggcc agccagcgga cggcctttga ctggggcgcg
     5581 ttctggttga aggtgctgtc tccgctcgcc ggcatggggt tgctcgtcgg gctgtgggcg
     5641 ctggcgacga tgaacagcac cacgttcccg tcgccgaccg agaccttcaa ggcggccgtc
     5701 gagttgtttg ccgatccgtt ctatcgcaag ggacccaacg accaggggat cgggtggaac
     5761 atcctcttct cgctcgagcg tgtggccatc ggcttcggcc tggcagcggc tgtcggcatt
     5821 ccgatgggct tcatgatcgg acgattcaag gtgctcaacg acatgctgtc gccgctgatc
     5881 agcctgctgc ggccggtatc accactggcc tggctgccga tcggcctgct ggtcttcaag
     5941 tcagccaatc cggctgcggt ctggaccatc ttcatctgct ccatctggcc catgatcatc
     6001 aacaccgccg tgggtgtgca gcgtgtgccg caagactact tgaacgtggc ccgcg
//
LOCUS       DAOORV010000194         5992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16078_length_5992_cov_5.807310, whole genome shotgun sequence.
ACCESSION   DAOORV010000194 DAOORV010000000
VERSION     DAOORV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16078_length_5992_cov_5.807310"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKV56_16735"
     CDS             <1..311
                     /locus_tag="PKV56_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOX69507.1"
                     /translation="TPEGSVMLDVDVNKDSVGQATAAGFAIDTKHVKTQVLVENGGTV
                     VIGGIFEQNDRTDITKVPFLGDVPFLGNLFKTTSKSSQRTELLIFLTPKVVTERTAAQ
                     "
     gene            356..1996
                     /locus_tag="PKV56_16740"
     CDS             356..1996
                     /locus_tag="PKV56_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69508.1"
                     /translation="MKLVKAIWILMCASLLASCGGSSGAGDSPFNPNDGSGGSSAVAD
                     LVLVLSAAQLANTGSATVTITATAIDASRNALADVPVTVTADSDAIVSSTATATGTDG
                     TLTASLSIGANRSNRVITVSAVSGTVVKSATVQVVGTTISSVLAPAVIAPSAAGNVQY
                     RVVDQAGNPMVNQPVQVTAAGLNPAQANGTTGANGDFVFNYTAPAAAGSFQINAIIAG
                     ASDVQTVQVQTTSTVPNVTAAITSASVSANPSVVAVNLAGSSSNRSEIRALFLGAGNV
                     PIPNVRVRFDLDGDAQNIGGTFTAGSGTQTLYSDANGVVTTAYVPSTRSSPTNGVTVR
                     ACYGVSDTDPNLLNCTTNAVQTLTVTSEPLSVTIGTNAKIVVGSLTYTKQFNVVVVDS
                     SGAVKPDVNIVASIDLPFYRKGFYTAVTDGWARTETAVCASEDSNRNGVLETGEDING
                     NGSLEPRKADVSVRLLSSRTDASGSAILEVTYNQDHGSWVDAVITVSASGIAGTEGRA
                     SYALAPVPVDAAAIKDTTNPPAFVVSPYGTAASCSSPN"
     gene            2002..2571
                     /locus_tag="PKV56_16745"
     CDS             2002..2571
                     /locus_tag="PKV56_16745"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOX69509.1"
                     /translation="MPTSIALIGMPGGGKSTVGRHLARRLGWGFVDSDALIEQRLGES
                     IRGFFEREGEDRFRDIEQATIDELTSQGGTRVLATGGGVCLREANRHVLHERCTVVYL
                     RSTPEELHRRLRHDTHRPLLQVADPLGRLRELFGKRDPLYRECSHFVIETGRPSVQTL
                     VNMILMQLELAGVVEPSLVHSPVDPPGRP"
     gene            2609..3703
                     /gene="aroB"
                     /locus_tag="PKV56_16750"
     CDS             2609..3703
                     /gene="aroB"
                     /locus_tag="PKV56_16750"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOX69510.1"
                     /translation="MSVSKTERVSIELADRSYDILIGAGVLDAPDSYEGLPRAADALV
                     VTNTTVARLYAARLVRALTARYRRVHVVELPDGELHKDWVTLNLIFDALLSHGCDRKT
                     VLFALGGGVVGDMTGFAAASYMRGVPFVQVPTTLLAQVDSSVGGKTAINHPMGKNMIG
                     AFYQPARVIADLQTLSTLPRREVAAGLAEVIKYGPIADDEFFRWIEAHVDQLLALDPA
                     ALAHAVKRSCEIKADVVGQDEREAGLRAILNFGHTFGHAIESGLGYGQWLHGEAVGCG
                     MMMAAELSRRLGLIDAAYVQRLRALVERCGLPVRGPVLGADRYLELMRLDKKSEGGEI
                     RFVVIESPGRAGVRAVPDALVREVLADTCG"
     gene            3723..4886
                     /locus_tag="PKV56_16755"
     CDS             3723..4886
                     /locus_tag="PKV56_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOX69511.1"
                     /translation="MRAEYASRPEATRGRRHPEPAAPPRDDFQRDRDRIVHSTAFRRL
                     VYKTQVFLNHEGDLFRTRMTHSLEVAQLGRSIGRALGLNEDLIEAIALAHDLGHTPFG
                     HAGQDALHNRMTEVQPDGGGFEHNFQSLRVVDRLEERYAAFDGLNLSFETREGILKHC
                     SRRNAQWLVSNDPTGVGRRFIDRSEPSLEAQLCNLADEIAYNAHDIDDGVRSGLLTLD
                     QLEALSLPQRFLQQVRVDHPRLTGRRVLFETLRRMLSQQVYDVVESTRQALLEHGVCD
                     PDDARRLPTMVRFSPEMRRLTDEVQRFLRANLYRHPQVAATTGLARTVLEDLFDRYVD
                     DPSQMHAQELGARSVERAVADYVAGMTDRFALQEHARLTGRPLDWPARPRAAD"
     gene            complement(4971..>5992)
                     /locus_tag="PKV56_16760"
     CDS             complement(4971..>5992)
                     /locus_tag="PKV56_16760"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOX69512.1"
                     /translation="NNLGYTLGKPEITKLKDGTWVVIFASGYNNVSPGDGRGRLFVVN
                     AATGSLIRTIDTGVGDTTTPSGLAQIRAWVDRTEINNTSLRVYGGDNLGNVWRFDINN
                     DIGAAGYDAQLLATLRGGAGNAQPVTARPELGLVNGNAVVYVGTGRYLGVTDLTDNSA
                     QSLYAIKDSLGSTSLGNPRGASFVQQTLTGGTCPADATVCDPSQTVRLGTSNAVDFAT
                     NNGWFIDLPVTRERANTDPQLALGTLVYTTNIIDPDACSVGGTSFINFFDYRTGGPVT
                     SSTGVTSVLLGNAIATRPTIVRLPSGKVISLTRLSDARTVVSRIPVGTVPGTTRRISW
                     RELAN"
BASE COUNT         1061 a         1943 c         1966 g         1022 t
ORIGIN      
        1 tcacgccgga aggcagcgtc atgctggacg tcgacgtcaa caaggacagc gtcggccagg
       61 cgaccgcagc gggcttcgcg atcgatacca agcacgtcaa gacgcaggtg ctggtcgaga
      121 atgggggcac cgtggtgatc ggcgggatct tcgagcagaa cgatcgcacc gacatcacga
      181 aggtgccgtt cctgggcgat gtgccgttcc tgggcaacct gttcaagacc acgagcaagt
      241 catcgcagcg aaccgagctg ctgatcttcc tcacgcccaa ggtcgtgacc gaacgcactg
      301 ccgcgcagtg atcggcgccg tcacctaaac cattgcaaga gagcgagtcc gagtgatgaa
      361 gctggtcaaa gcgatctgga tccttatgtg cgcgtccctg ctggcgtcct gcggcggcag
      421 cagcggtgcg ggcgactcgc cgttcaaccc caacgatggt tcggggggga gttccgcggt
      481 ggcagacctg gtcctcgtac tgagcgcggc ccagctggcg aacacgggct ctgcgacggt
      541 gactatcacg gcaacggcca ttgatgcctc gcgcaatgcg ctcgcggatg tcccggtgac
      601 agtgaccgct gattccgacg ccattgtttc gtctactgcc accgccacag gaacggacgg
      661 aaccctgaca gcgtcactct caatcggtgc caatcgatcc aatcgcgtga tcacggtgag
      721 tgcggtgtcc ggcaccgtcg tgaagtccgc gactgtccag gtggtcggca ccacgatcag
      781 cagcgtcctg gcccccgcag tgattgcccc ctcggcagcg ggtaacgtgc aataccgcgt
      841 agtcgaccag gctggcaatc cgatggtcaa tcagccggtg caagtcaccg ctgcgggttt
      901 gaacccggcc caggccaatg gcacgacggg cgcgaacggc gatttcgtct tcaactacac
      961 cgcgccagct gctgccggat cgttccagat caatgcgatc atcgctggag catccgacgt
     1021 ccagactgtg caagtccaaa ccaccagcac ggttcccaac gtgacggctg caatcacttc
     1081 agcttcggtg tccgccaacc cgagcgtcgt cgcggtcaat ctggcgggca gcagttcgaa
     1141 caggtccgaa atccgtgcgc tgtttctcgg cgcaggcaat gtgcctatcc cgaacgtgcg
     1201 ggtgcgcttt gacctcgacg gagatgcgca gaacatcgga ggcacgttca cggctggcag
     1261 cggcacgcag actctctaca gcgacgccaa cggcgtggtg acgacggcgt atgttccgag
     1321 cacgcgatcg agtccgacca atggcgtaac cgtgcgtgcc tgctacggtg ttagtgacac
     1381 tgaccccaat ctcctgaact gcacgaccaa tgcggttcag acactgacgg tgacctccga
     1441 gcctctgagc gtgacgatcg gcacgaacgc caagatcgtc gtcggatcac tgacctacac
     1501 gaagcaattc aacgtggtcg ttgtcgactc atccggtgcc gtaaagccgg atgtgaacat
     1561 cgtcgcttcc atcgacctgc cgttctatcg caagggcttc tacaccgccg tcactgatgg
     1621 ctgggccagg accgaaaccg ccgtgtgtgc cagcgaggat tccaaccgca acggcgtgtt
     1681 ggaaaccggg gaagacatca acggcaacgg atcgctggag ccgcgcaaag cggacgtgag
     1741 cgttagactg ctgtcgagca ggactgatgc gtcaggcagt gccatcctcg aagtcaccta
     1801 taaccaggac catggcagct gggtcgatgc agtgatcaca gtttctgctt cgggcattgc
     1861 cggaactgaa ggccgagcta gttatgcgct ggcgccagtg ccagtcgatg ctgctgcgat
     1921 caaggacaca acgaatccgc cggcctttgt cgtgagtccg tacggcacgg ccgcgagctg
     1981 ctcaagtccg aactgaggaa tttgcccacc tccatcgcgc tgatcggcat gcccggcggt
     2041 ggcaagtcca ccgtcggccg ccatctggcg cgacggctgg gctggggttt cgtcgactcg
     2101 gacgcgctga tcgagcagcg gctcggtgaa tcgatccgag gcttcttcga acgagaaggc
     2161 gaagatcggt ttcgcgacat cgagcaggcg acgatcgacg aactgacatc ccaaggcggc
     2221 acacgtgtgc tggccaccgg gggaggggtt tgtcttcgcg aggccaaccg ccatgtgctg
     2281 catgagcgct gcacagtggt ctatctgcga tcgacacccg aagaactgca ccggcgactc
     2341 aggcacgata cgcaccggcc gctgctgcag gtggccgacc cgctggggcg cctgcgcgaa
     2401 ctcttcggca agcgcgatcc tctgtatcgc gagtgcagcc atttcgtgat cgagacgggg
     2461 cggccctcgg tccagacgct ggtcaacatg atcctgatgc agctcgaact cgccggtgtc
     2521 gtggagccca gcctggtgca ttcgcccgtc gatccgcccg gcagaccctg aggcggaggc
     2581 ggcccggagt gcgccttaca ctgggcgcat gagtgtctcc aagaccgagc gggtcagcat
     2641 cgaactcgcg gatcgcagct acgacatcct gatcggcgcc ggcgtgctcg acgcgcccga
     2701 cagctacgaa gggctgccgc gtgcagccga tgcgctggtc gtcaccaaca cgactgtcgc
     2761 gcgtctgtat gcggcccggc tcgtccgggc gctcactgct cgataccgca gggttcatgt
     2821 ggtcgagttg cccgacggcg aactgcacaa ggattgggtc acgctgaacc tgatcttcga
     2881 tgccctgctc agtcacggct gcgaccgaaa gacggtgttg ttcgcgctgg gcggtggcgt
     2941 ggtcggtgac atgactggtt tcgccgcggc gagctacatg cgtggtgtgc cgttcgttca
     3001 ggtgcccacc acattgctgg cgcaggtgga ttcatcggtc ggcggcaaga cagcgatcaa
     3061 ccacccgatg ggcaagaaca tgatcggcgc gttctaccag cctgcccggg tgatcgccga
     3121 cctgcagacc ttgtccaccc tgcctcgccg cgaagtggcc gcgggtcttg ccgaagtcat
     3181 caagtacggt ccgatcgccg acgacgagtt cttccgctgg atcgaggcgc acgtcgacca
     3241 gttgctcgcc ctggaccccg cggcgctggc gcacgccgtg aagcgttcct gcgagatcaa
     3301 ggccgacgtg gtcggccagg acgagcgcga ggcgggcctg cgcgccatcc tcaacttcgg
     3361 ccacaccttc ggccacgcga ttgagtcggg cctgggctac ggccaatggc ttcacggcga
     3421 agccgtaggc tgcggcatga tgatggccgc ggagctgtcg cgccgactcg gcctcatcga
     3481 cgcagcctac gtgcagcgtc tgcgcgcgct ggtcgaacga tgcggactcc cggtgcgcgg
     3541 gcctgtgctc ggggcggatc gctacctcga attgatgcgg ctggacaaga agtcggaagg
     3601 cggagagatc cgcttcgtcg tgatcgagtc gccgggccgg gccggtgtca gagcagtgcc
     3661 cgacgccctg gtgcgagagg ttctcgccga cacctgcggc tgagccgacc tgcaccccac
     3721 gcatgcgcgc cgagtacgcc agccgacccg aggcgacgcg tggccgccgt caccctgagc
     3781 ccgctgcgcc accacgcgac gacttccagc gagaccgcga ccgcatcgtg cacagcacgg
     3841 cgttccgtcg cctggtctac aagacgcagg tattcctcaa ccacgaagga gacctgttcc
     3901 gcacccgcat gacgcactcg ctcgaggtgg cgcagcttgg acgctccatc ggccgtgcat
     3961 tgggcctgaa cgaagacctg atcgaggcca tcgccctggc acacgatctt ggccacacgc
     4021 cattcgggca cgcggggcag gacgcactgc acaaccgaat gacggaagta cagcccgatg
     4081 ggggcggctt cgagcacaat tttcagagcc tgcgcgtggt cgatcggctc gaagagcgct
     4141 acgccgcttt cgatgggctg aacctgagtt tcgagacgcg cgaaggcatc ctcaagcact
     4201 gcagccgccg taatgcgcag tggctggtct ccaacgatcc caccggcgtc ggccggcgct
     4261 tcatcgaccg cagcgagccc agcctggaag cacagctgtg caatcttgcc gacgaaatcg
     4321 cctacaacgc ccacgacatc gacgatggcg tgcgatcggg gctgctgacg ctggaccagc
     4381 tcgaagcgct gtctctcccg cagcggttcc tgcagcaggt ccgagtcgat caccccaggc
     4441 tgaccggtcg ccgggtgctg ttcgagacat tgcgccgcat gctctcgcag caggtctacg
     4501 acgtcgtcga gagcacccgt caggcgcttc tcgaacacgg cgtgtgcgac ccggacgacg
     4561 cgcggcgact gcccacgatg gtgcgcttct cgccggagat gcgccggctc actgacgaag
     4621 tgcagcgctt cctgcgcgcc aacctgtacc ggcacccgca ggtcgcggcc accaccggcc
     4681 tggcgcgcac ggtgctggag gatctgttcg accggtacgt cgacgaccct tcgcaaatgc
     4741 acgcccagga gttgggcgcg cgcagcgtcg aacgcgccgt ggcggactac gtcgccggca
     4801 tgacggaccg cttcgcgctg caggagcacg cccgtctcac gggccggcca ctcgactggc
     4861 cggcgcggcc gcgcgctgcc gattgacgcg cgtacacgcg gactgcctcg ccaacaagaa
     4921 aaacgggccc cagcatcgtg ccggggcccg cgtttcggcg aagaccttgt tcagttcgcc
     4981 agttcgcgcc aggagatgcg acgcgtcgtt cccggcaccg tgccgaccgg aatgcgcgag
     5041 accaccgtgc gcgcgtcgga cagacgcgtc aggctgatca ccttgccgct gggcagccgc
     5101 acgatggtcg gccgcgtcgc gatggcattg cccagcagca ccgaggtgac gccggtcgac
     5161 gaggtcaccg gcccgccggt gcggtagtcg aagaagttga tgaagctcgt gccgcccacc
     5221 gaacaggcat cggggtcgat gatgttggtg gtgtagacga gcgtgcccag tgcgagttgc
     5281 gggtcggtgt tggcccgttc gcgggtcacc ggaaggtcga tgaaccagcc attgttggtg
     5341 gcgaaatcga cggcgttcga cgtccccagg cgcaccgtct gcgacggatc gcagaccgtc
     5401 gcgtccgccg gacaggtgcc tccggtcagg gtctgctgga cgaagctggc cccgcgcggg
     5461 ttgcccaagc tggtggaccc gagtgagtcc ttgatcgcat acaggctctg ggcgctgttg
     5521 tcggtgaggt cggtgacgcc gaggtagcgg cccgtgccca cgtagaccac cgcgttgccg
     5581 ttcaccaggc cgagttccgg ccgagccgtc acgggctggg cgtttccggc cccaccacgc
     5641 agcgtggcca gcaattgggc gtcgtagcct gccgcgccga tgtcgttgtt gatgtcgaag
     5701 cgccagacgt tgccgaggtt gtcgccgccg taaacgcgca gcgaggtgtt gttgatctcg
     5761 gtgcgatcga cccaggcgcg gatctgggcc aggccgctcg gcgtggtggt gtcgccgacg
     5821 ccggtgtcga tggtgcggat cagcgagccg gtggcggcat tgaccacgaa caggcggcca
     5881 cggccgtcgc cgggcgagac gttgttgtag cccgaggcga agatcaccac ccaggtaccg
     5941 tccttgagct tcgtgatctc cggcttgccc agcgtgtagc ccaggttgtt gt
//
LOCUS       DAOORV010000195         5975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16135_length_5975_cov_9.503547, whole genome shotgun sequence.
ACCESSION   DAOORV010000195 DAOORV010000000
VERSION     DAOORV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16135_length_5975_cov_9.503547"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1962)
                     /locus_tag="PKV56_16765"
     CDS             complement(79..1962)
                     /locus_tag="PKV56_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69513.1"
                     /translation="MPALQHQVLDRPVATEAEHADDRGWFLLGSKWEDDVWVLAPGNA
                     LEERRPVRLRWDFELQDGRRFTDERYATLRETSKQLIALIRTHSMFTGLPLRPSTVAT
                     YFFTLRCLLQWMEREHFSRFADLDPPALPQFQQWLRTRPVAGHSSPRAPGTVLRHLYL
                     FEYLHRFGAELDDCLSFDPFAGHDHRRAAGYHEGLRQPWPYTPDSVAVILLQAAIDIV
                     TREAPIILQAWQTYRKAAAGGRGAGHAHTGRATRALRNASAGIPDAQSPVRSVRELDL
                     RTDLLYAACFVVLSYLVGPRVSEILHLKAGCVQRRCADGVGEPVTVIVGAIFKRQSGY
                     HGRAHEWVAPPAAVQAVAALEGLSAAQRAVAGRDELWLRRWHGNGATEWHEVRPDLLE
                     IPSISRINIQLQRFAAWLGLTHQDRPWRLSTHQGRKTFARFAALRDRTCLFALAQQLG
                     HRERAETDHGYAGSDYRLAQEIDAEILQQSVNAWEHMLAAPGLGGRAGEEIVANRPRF
                     RGKRMKEDLASYARLLVDAGLVLGVCDWGFCVYREAYSACLGNAAGPNPARREPSTCA
                     RCKNFAVSQRHRPYWVEQLHRCERLLDEPALPRQTLRIVRARLDEARSLVGTIDSDGK
                     ERS"
     gene            complement(1973..3715)
                     /locus_tag="PKV56_16770"
     CDS             complement(1973..3715)
                     /locus_tag="PKV56_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69514.1"
                     /translation="MNSPRSSIRRPLSHEPVATPVDGVQPARFDAPRLVRRTVIDFSS
                     LSLPEDVRLALAQAFWSHCGARSPRGIKSSWSTVLTFNRFAVESGAVSSLASLDRQLL
                     VRYIEWLNAQRRADGTPWAISTRASTYGALRQLLRWLERCRPDLVDRIEHPFTPFPGR
                     GSDRPRRATLTARELRAILQACEVEIAALRQLRASAAEQRAADGDKPGSLGWLLAEVD
                     RRFHGIVPSKLELDRRGNHRMQAAVRRWGGAKQIEPFLYPRAVSLLPYYLAILIHAAG
                     NPEPIVELGRDCLQDVPLLENRQALVWFKARAGRLQRRTFERNAPFEPPALVRDILAW
                     NERLRPLAPPAQRDRLFIFKGLRTVNAMSTITVHHLLSQFCERHGLPAFALASIRPSV
                     LCSFYRATGDLVRTRAVANHANVATTVRYVEAPVVRRHNHMRIAALQEAFLEHLTAAP
                     NTPTPQAAAVAAPSALPPGELVSMFGFDCRDPLAGTAPGTRPGELCTNFMGCFTCPNA
                     VITPAPLTMARLLQARDHLRAAAATLHPARWQAFYAPQLRILEEDILPRFSARELAAA
                     DLLMPRLPPLPDLR"
     gene            complement(3712..4902)
                     /locus_tag="PKV56_16775"
     CDS             complement(3712..4902)
                     /locus_tag="PKV56_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69515.1"
                     /translation="MVQIIRKASLPTPDEVAARTRAAAEQARAVTVREPRPSGFPLLF
                     TRQWQLIEPAVAFLHEHSVQRAHTDDTLRTYAEILYDWFETLEQNDIAWDSADAVDLV
                     AYRNRMMAQPSPHTDRPYRTSTINHRVRGVLRFYEWAVRTHWLHASPLADKTRDFQLA
                     RHYRPTQASHAQTYERSLFILRQYEAMPRPLVSEQARELLAALAPPYDLMARWQLYTG
                     LRVSELLRLSLSDIGARPASSTLHHSIEVVRKGRKPGHVIAPASLLDETDGYVSGHRF
                     AWFARARRRGRIVDHKALFINARGAPAGKNAYQRAVSQAGVACGFKATTHLLRATFAC
                     MLLARLEHLVDRGAKINPLIVVKVLLGHEHVETTDRYLRAIAVDPCVLKDVLDTLLSQ
                     EVVP"
     gene            complement(4859..5161)
                     /locus_tag="PKV56_16780"
     CDS             complement(4859..5161)
                     /locus_tag="PKV56_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69516.1"
                     /translation="MPACDALELLSAHVKSDPTYRPVKDDQSQRWHLSTDRGDFEIVT
                     TGPKWYDTRAKRGGGGAIDLAIHLLDVPFVEAVKLLNGKAVPHGADHPQGLAADSR"
     gene            5347..>5975
                     /locus_tag="PKV56_16785"
     CDS             5347..>5975
                     /locus_tag="PKV56_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HOX69517.1"
                     /translation="MTVANATFHPYLVMPSRAGADGLGAFSYYDHVFLPESTKELVWR
                     HGGKRYKSQLVLRVNGKKKTEAFLFEHGGPGWAPVVLRDGTVSDGKVETYEKAVAEIL
                     CPADTFFTSVFSAQGKRPLSAFKNAEIKTLLADLLGLEQVRQQGALAADVVKQLKAGL
                     AVVRQGLARAQEDAAGTRRSLAELDGASQALLAATAQRTSTAARLDAAR"
BASE COUNT          995 a         1897 c         2141 g          942 t
ORIGIN      
        1 gctgtcgaac catcatcgtc agcgcctcgc gcagacgctg ggccgatgcg gtcttggtgg
       61 cgccgcgcgc ctgcttcgtc atgatcgctc cttcccatcg ctgtcgatcg tgccgaccag
      121 cgaccttgcc tcatcgagtc gtgcgcggac gattcgcagg gtctgccttg gcagggcagg
      181 ctcgtccagc aaccgctcgc agcggtggag ttgctcgacc caatagggtc gatgccgctg
      241 cgacaccgcg aagttcttgc agcgcgcgca ggtcgacggc tcgcggcgag cggggttcgg
      301 gccggccgcg ttgccgaggc aggcgctgta cgcctcgcga tagacgcaaa agccccaatc
      361 gcagacgccg agcacgagtc ccgcgtccac cagcaggcgc gcgtagcttg cgaggtcttc
      421 cttcatgcgc ttgccccgaa agcgtgggcg attggccacg atctcttcgc cggcccgtcc
      481 gcccaggccg ggggccgcca gcatgtgttc ccaggcgttg accgactgct gcaggatctc
      541 ggcgtcgatc tcctgggcga gccggtagtc ggacccggcg tagccatggt cggtctcggc
      601 gcgctcgcga tgaccgagtt gctgggccag cgcgaataga cacgtgcgat cacgcaacgc
      661 cgcgaacctg gcgaaggtct tgcggccctg gtgcgtggag agccgccacg gccggtcctg
      721 gtgcgtcagt cccagccagg cagcaaagcg ctgcagttga atgttgatcc gtgagatcga
      781 tggaatctcg agcaggtcgg gccgcacctc gtgccactcg gtcgccccgt tgccgtgcca
      841 gcggcgcaac cacagctcgt cgcgtcccgc gacggcgcgc tgcgcggcgg acaacccttc
      901 gagtgccgcc acggcctgca ccgcggcagg tggcgcgacc cattcatgcg cgcggccgtg
      961 gtagcccgat tggcgcttga agatcgcccc gacgatcacg gtgacgggtt cgcctacacc
     1021 gtcggcgcaa cggcgctgca cgcagccggc cttcaggtgc aggatctcgc tgacgcgcgg
     1081 gccgaccaga taggagagga cgacgaagca ggcggcgtag agcaggtcgg tgcgcaggtc
     1141 gagttcgcgc accgatcgaa ccggggattg cgcatcggga atgccggcac tggcgttgcg
     1201 aagcgcgcgt gttgctcgcc cggtgtgcgc atggcccgca ccgcggccac cggcggccgc
     1261 cttccgatac gtctgccagg cctgcagaat gatgggtgcc tcgcgcgtga cgatgtcgat
     1321 cgccgcctgc aacaagatca ctgcgacgct gtccggcgtg tagggccaag gttgccgcag
     1381 tccctcgtgg tagccggcgg cccggcgatg atcgtgtccc gcaaaggggt cgaaggatag
     1441 gcagtcgtcg agctctgcgc caaatcgatg gaggtactca aacaggtaca ggtggcgcag
     1501 cacggtcccg ggtgctcgcg gcgaggagtg acctgccacg gggcgggtgc gcagccattg
     1561 ctgaaactgc ggcaacgccg gcgggtcgag atcagcgaag cggctgaagt gctcgcgctc
     1621 catccattgg agcaggcagc gcagggtgaa gaagtacgtc gccacggtgc tcgggcgcag
     1681 tggcagccca gtgaacatgg agtgcgtccg gatgagggcg atgagttgct tgctcgtctc
     1741 gcgcagcgtg gcgtagcgct cgtcggtgaa ccgccggccg tcctgcagtt cgaagtccca
     1801 gcgcagacgc acaggccggc gttcttccaa ggcgttgccg ggtgccagaa cccaaacgtc
     1861 gtcctcccac ttcgacccaa gaaggaacca gccgcgatcg tcggcgtgtt cggcctcggt
     1921 ggcgacagga cgatcgagga cttggtgttg cagggcaggc acgacgtggc cctcatcgca
     1981 agtccggcag cggcggcaac cgcggcatca acagatcggc cgccgcgagc tcccgagcgg
     2041 agaagcgcgg caggatgtct tcttcgagga tgcgcagttg gggcgcgtag aaggcctgcc
     2101 agcgcgccgg atgcagcgtc gccgccgcgg cgcgaagatg gtcacgcgcc tggagcagac
     2161 gagccatggt cagcggtgcc ggcgtgatca cggcgttggg gcaggtgaag caacccatga
     2221 agttggtgca gagctcgcca gggcgtgtac ccggcgcggt gccggccagc ggatccctgc
     2281 agtcgaagcc aaacatcgac accagctcgc ccggcggaag cgcgctcggc gccgccactg
     2341 cggccgcctg cggagtcggc gtgttcggcg ccgcggtgag gtgctcgagg aaggcctcct
     2401 gcagcgcggc gatgcgcatg tggttgtgcc ttcgcacgac cggcgcctcg acatagcgaa
     2461 cggtcgtcgc gacgttggcg tgattggcca ctgcccgcgt tcgcaccaga tcgccggtgg
     2521 cgcggtagaa gctgcacaga acgctcggcc ggatgctcgc caacgcaaac gccggcaggc
     2581 cgtggcgttc gcagaattgc gaaagcaagt ggtggaccgt gatggtggac atcgcgttca
     2641 cggtgcgcag ccccttgaaa atgaacaggc ggtcgcgttg cgccggcggc gccagcggcc
     2701 gcagccgctc gttccaggcg aggatgtccc gcaccaacgc cggcggctcg aacggggcgt
     2761 tgcgttcgaa cgtacgccgc tgcaggcgac cggcgcgggc cttgaaccac accagcgcct
     2821 ggcggttctc cagaagggga acatcctgca ggcagtcgcg cccgagttcg acgatgggct
     2881 cggggttgcc ggccgcatgg atcaggatcg ccaggtagta cggcagcagt gagacggcac
     2941 gtgggtacag gaacggttcg atctgcttgg cgccgcccca gcgacgcacc gccgcctgca
     3001 tccgatggtt gccgcgccgg tccaactcga gcttgctggg cacgatgccg tggaagcgcc
     3061 gatcgacctc ggccaggagc caacccaagg aaccgggctt gtcaccgtcc gcggcgcgtt
     3121 gctcggcggc cgatgcgcgg agctgccgaa gggcggcgat ctccacttcg caggcctgca
     3181 ggatggcgcg aagctcgcgt gccgtcagcg ttgcgcgtct gggtctgtcg ctgccccgcc
     3241 ctgggaaggg cgtgaagggg tgctcgatgc ggtcgaccag atcggggcgg cagcgctcga
     3301 gccagcgaag cagttggcgc aaggcaccgt aggtgctggc ccgcgtggag atggcccagg
     3361 gcgtgccgtc cgcgcgtcgt tgcgcgttga gccactcgat atagcgcacg agcagctggc
     3421 ggtcgaggct cgcaagcgac gagacggctc ccgattcgac cgcgaagcgg ttgaaggtca
     3481 gcaccgtgct ccaggatgac ttgatgccgc gcggcgagcg ggctccgcaa tgactccaga
     3541 acgcctgcgc cagcgcaagc cgcacgtcct caggcagtga caacgatgag aagtcgatca
     3601 ccgtccggcg aacaaggcgc ggggcatcga agcgcgcggg ttgcacaccg tctactggcg
     3661 tcgccacggg ttcgtgcgac agaggccggc ggatgctcga acgcggcgag ttcatggcac
     3721 cacttcctgc gatagcagcg tgtcgagcac gtccttgaga acgcatgggt cgacggcgat
     3781 ggcgcgaagg tagcggtcgg tggtctcgac gtgctcgtgg ccgagtagca ccttcacgac
     3841 aatcagcggg ttgatcttgg cgccacggtc caccaggtgt tcgagccggg cgagaagcat
     3901 gcacgcgaag gtggcccgca gcaggtgcgt ggtggccttg aacccgcagg caacaccagc
     3961 ttggctcacg gctcgctgat aggcgttctt gccagcaggg gcgccgcggg cattgatgaa
     4021 tagcgccttg tggtcaacga tgcgacccct gcgtcgggcg cgcgcgaacc aagcgaagcg
     4081 atggccgctg acgtagccat cggtctcgtc gagcaggctg gccggcgcga tcacatggcc
     4141 tggcttgcgg cccttgcgaa cgacctcgat ggagtgatgc agcgtcgatg acgccgggcg
     4201 ggcaccgatg tcgctgagac tgaggcgcag gagctcactc acccgcaggc ccgtgtacag
     4261 ctgccagcgg gccatcaggt cgtaaggcgg cgcgagcgcc gcgaggagtt cgcgcgcctg
     4321 ctcggagacc agaggccgcg gcatcgcctc gtattgccga agaatgaaga ggctgcgttc
     4381 gtaggtctgc gcgtgactgg cctgcgtcgg ccgatagtgt cgtgcgagct gaaagtctcg
     4441 cgtcttgtcg gccagcggcg aggcgtgcag ccaatgcgtg cgaaccgccc actcgtagaa
     4501 gcgaagcacg ccgcgaacgc ggtggttgat ggtcgacgtc cggtacggcc ggtcggtgtg
     4561 cgggctcggc tgggccatca tgcggttgcg ataggccacg aggtcgacag catcggcgct
     4621 gtcccacgcg atgtcgttct gctcgagcgt ctcgaaccag tcgtagagga tctcggcgta
     4681 ggtcctcagc gtgtcgtcgg tgtgggcgcg ctggaccgaa tgttcatgca ggaaggcgac
     4741 cgccggctcg atcagctgcc attggcgcgt gaacagaagc ggaaatccgg atgggcgggg
     4801 ctcgcgcacg gtgacggcgc gcgcctgctc tgcggcggcg cgagttcgcg cggccacctc
     4861 atcgggagtc ggcagcgagg ccttgcggat gatctgcacc atggggcact gccttgccgt
     4921 tcagaagctt gacggcttcg acgaacggca catccaagag gtgaatcgcc aagtcgatgg
     4981 cgccgcctcc accacgcttc gcgcgcgtgt cgtaccactt gggaccagtc gtcacgatct
     5041 cgaagtcgcc gcggtcagtg ctcaggtgcc agcgctggct ctgatcgtcc ttgaccggcc
     5101 gatacgtcgg gtcagacttg acatgggcgg acagcagctc gagcgcgtcg cacgccggca
     5161 tctggcgaag ccttgtcaac gtcgaggcac tgaacgatct acgttggcgg cgcgcgacca
     5221 ttgccacaat ctccggcagg gttgactcaa aacgcgggac gcggcgtgct actcgcctct
     5281 gaaagaggca tgggctgccc gggtgtgaga tcactgtagg cggcgccgct gatcaatcca
     5341 aggacgttga cagttgcgaa tgcaaccttc cacccctacc tcgtgatgcc gtcgcgggcc
     5401 ggtgcagacg gcctgggcgc gttctcctac tacgaccatg tcttcctgcc cgagagcacc
     5461 aaggaactgg tctggcggca cggcggcaag cgctacaaga gccagctggt gctgcgggtc
     5521 aacggcaaga agaagaccga ggccttcctg ttcgagcacg gcgggcccgg ctgggcgccg
     5581 gtggtgctcc gcgacggcac cgtgtcggac ggcaaggtcg agacgtacga gaaggccgtc
     5641 gcagagatcc tgtgcccggc cgacaccttc ttcacgtcgg tcttctctgc ccaagggaag
     5701 cggccgctgt cggcgttcaa gaacgcggaa atcaagacgc tgctggccga tttgctgggg
     5761 ctggagcagg tgcggcagca aggggcgctc gcggcggacg tcgtcaagca gctcaaggct
     5821 ggcctggctg tggtccggca gggtctggcg cgagcgcagg aggacgcggc gggcacgcgg
     5881 cgcagcctcg cggagcttga cggtgcgtca caggccttgc ttgcggcaac ggcccaacga
     5941 acttcgacag ccgcgaggtt ggacgctgcg cgcca
//
LOCUS       DAOORV010000196         5941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16243_length_5941_cov_8.393136, whole genome shotgun sequence.
ACCESSION   DAOORV010000196 DAOORV010000000
VERSION     DAOORV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16243_length_5941_cov_8.393136"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKV56_16790"
     CDS             complement(<1..1097)
                     /locus_tag="PKV56_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOX69518.1"
                     /translation="MNAQEAKHSAAMPSAPAFGQADLTNCERELIHLAGSVQPHGVLL
                     ELSATELVVLQVSGNAASLLGIEPDALLQQTLAVLGGDVAGRVAEWQRNSDLREPVVL
                     QCRLGPAQRPFDCALHRLANGGLVLELEPSTSAPAGAQLHDLPPDVMAVILSDAMQRF
                     GAAPTIGVLADSVVEVIRNLTGYDRVVVYKFDPDGHGKVISEARHPRLESLLGHRYPA
                     SDIPHRARALYLLNRLRMLVDVDATQSPLLPAQPPLTVAPLDMSMCQLRSMSPIHLQY
                     LRNMGVTATLSASLVREGQLWGLIAAHHYTPRYLRRTVRAAAELLAEVASTRISAIEN
                     YAHAQVALMVRRLEQRLIEATSIEGDWRYAL"
     gene            complement(1098..2525)
                     /gene="ppsR"
                     /locus_tag="PKV56_16795"
     CDS             complement(1098..2525)
                     /gene="ppsR"
                     /locus_tag="PKV56_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HOX69519.1"
                     /translation="MSTTLELDLSPLSPLARELAATIAHVASDIALVIDADGVIATVA
                     AGAAVQEHGCNQWVGQHWIDTVSASTRPKIQSLMDEVASSGLTARRREVNHLSQIGDE
                     LPVTWSAIRLGADGPVLAVGRDLRAIAAIQQRFVEAQQDMERQYWNRRQAETRYRLLF
                     QVANDAVLLIDADSQCVLAANPACEELLGVPPTVMQGKALTDSLPQAARAKVAELLTT
                     ARSTGRAVEIRLRALSTGGMLEFSATPFRFEDERHLLVRARHGTAAGTGEDSLAFSRM
                     AEFVETMLEAVVITDSSGGVLMANQAFLRLTRQANDTSVRGRALPDLLLDTEGGWATL
                     VSRVLATGLISKAALQVGSLDGQPLSVQVSATLLAEGEQTCLGFVLRIDRRLPQDAAC
                     GDVMAGLLGQVGRVPLADLLVEVTHRAERQLIESALLRTGGLRAAAADALGMTPEALD
                     LRIQRHGLSSLGYADDAAATPPLIN"
     gene            complement(3078..3168)
                     /locus_tag="PKV56_16800"
     tRNA            complement(3078..3168)
                     /locus_tag="PKV56_16800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3132..3134),aa:Ser,seq:gga)
     gene            complement(3182..3550)
                     /locus_tag="PKV56_16805"
     CDS             complement(3182..3550)
                     /locus_tag="PKV56_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HOX69520.1"
                     /translation="MAANVLGTELIPCSYDPLTGFFRDGCCNTRADDTGTHVVCAKVT
                     KEFLAYSAERGNDLTTPHPEFRFAGLKPGDRWCLCVTRWLEALKAGVAPPVVLESTHR
                     KALDHVPLETLSAHALKPVG"
     gene            complement(3553..4470)
                     /locus_tag="PKV56_16810"
     CDS             complement(3553..4470)
                     /locus_tag="PKV56_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOX69521.1"
                     /translation="MEFAVVIAVIAIIFIVRTFKIVPQQNAWVVERLGKYDRTLTPGL
                     KFVVPFVERVAYKHSLKEVPLDVPSQICITRDNTQLQVDGIIYFQVTDPMRASYGSSN
                     YIVAITQLAQTLLRSVIGKMELDKTFEERDTINASVVNALDEAAANWGVKVLRYEIKD
                     LTPPAEILRSMQAQITAEREKRALIAASEGRKQEQINIATGEREAFIARSEGQRQAEI
                     NNAQGEASAIIAVADATAEAIRKIAEAIRSPGGEQAVQLKVAEKAVEAYAQLAQKNNT
                     MIVPGNMSEVSALIGTAMTLLKTKPPGAN"
     gene            complement(4506..4994)
                     /locus_tag="PKV56_16815"
     CDS             complement(4506..4994)
                     /locus_tag="PKV56_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOX69522.1"
                     /translation="MDFAHATFWWVAAGLAVAVELATGTFYLLMIALGLACGALAAHL
                     GLGGSMQVTVAAVVASGATALWHWKRMQHPHSLPARENRDVNLDIGETVQVVEWAPDL
                     TARVSYRGTAWTARLQPGCAAAAGMHRVTAVEGNWLVVAPASSASPSPADPATTAPHS
                     AL"
     gene            5159..>5941
                     /locus_tag="PKV56_16820"
     CDS             5159..>5941
                     /locus_tag="PKV56_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOX69523.1"
                     /translation="MLRFRFPIVIIDEDYRSENTSGLGIRALAQAIEKEGFEVLGATS
                     YGDLSQFAQQQSRAGAFILSIDDEEFTPGPELDPAVLNLRKFIEEIRFKNADIPIYIY
                     GETRTSQHLPNDVLRELHGFIHMFEDTPEFVARHIIREAKTYLDGLAPPFFKALMDYA
                     QDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHNGAIG
                     ASERNAARIFNADHCFFVTNGTSTSNKMVWHHTVAPGDVVVVD"
BASE COUNT         1062 a         1877 c         1926 g         1076 t
ORIGIN      
        1 aacagggcgt agcgccagtc gccctctatc gaggttgcct cgatgaggcg ctgctccaac
       61 cggcgcacca tcagcgccac ctgcgcatgg gcatagtttt cgatagccga gatgcgtgtc
      121 gatgccacct cagccaacag ttcggcagca gcgcgtaccg tgcggcgaag gtagcgcggt
      181 gtgtagtggt gcgccgcgat caagccccag agctggccct cgcgtaccag cgaggcgctc
      241 agcgttgcgg tcacgcccat gtttcgcagg tattgcaaat ggatgggcga catgcttctc
      301 agctggcaca tcgacatgtc cagcggggcg accgtgaggg gtggctgggc cggcagcagg
      361 ggagactgag tggcgtcgac gtctaccagc atacgcaagc ggttgagcag atagagagcg
      421 cgtgcgcgat gcggaatatc gctggccgga tagcggtgcc caaggagcga ttccagccgc
      481 gggtggcggg cttcggaaat gaccttgcca tgcccgtcag gatcaaattt gtaaacgacg
      541 acacggtcgt agcctgtcag gtttcgtatc acctccacca ccgaatcggc cagaacgccg
      601 atcgttggcg ctgcgccgaa gcgctgcatg gcatcggaca ggattacagc catcacatcg
      661 ggtggcaagt cgtgcaactg cgcgcctgca ggcgcgctcg ttgaaggttc gagctccagc
      721 accagaccgc cattggcgag gcggtgcagg gcgcaatcga agggtcgctg agccggtccg
      781 agccggcatt gcagcacaac gggttcgcgc aggtctgagt tgcgctgcca ctctgcaaca
      841 cgtccagcca catcgccacc cagaacggcc agcgtttgct gcagcaaggc gtctggctcg
      901 atacccagca gggacgctgc attgccgctg acctgcagca caaccagttc ggtcgcgctc
      961 aactccagca gcacgccatg gggctgcaca gatcccgcaa ggtggatcag ctcgcgctcg
     1021 cagttcgtga gatcggcttg gccgaaggca ggtgccgatg gcatggctgc cgagtgtttc
     1081 gcttcttgcg cgttcactta attgatcagc ggtggcgtgg ctgcggcgtc atccgcatag
     1141 cccaggctgg acaaaccgtg tcgctgaata cgcaaatcga gcgcctcggg cgtcatgccg
     1201 agcgcgtccg ccgcggctgc acgcaagccg ccggtgcgta gcagggcgct ctcgatgagc
     1261 tggcgctcag cccgatgggt cacctcgacg agcaaatcgg ccagcggcac acgcccaacc
     1321 tgaccaagta atccggccat cacgtcaccg cacgctgcgt cctgtggcag gcgtcgatcg
     1381 atgcgcagaa cgaatccgag gcaggtctgc tcgccctcgg ccagcaaggt tgccgatacc
     1441 tgcacgctca gcggctggcc atccagcgaa ccaacctgaa gcgcggcttt tgatatgaga
     1501 ccggtggcga gcactcggga caccaatgtg gcccagccac cttccgtatc gagcagaagg
     1561 tcaggaagtg ctcggccgcg cacgctggtg tcattggctt gacgggtcaa acgcaagaat
     1621 gcctggttag ccatcaggac accgcccgac gagtcagtga tcacgactgc ttccagcatc
     1681 gtctcgacaa actcagccat ccgagaaaag gccaggctgt cttcgccagt tcctgcggca
     1741 gtgccatgcc gtgcgcgcac caataggtgc cgctcatcct cgaatcggaa aggcgtggct
     1801 gagaattcca gcatcccgcc ggtgctcagg gcccgcaatc ggatctcgac tgcgcggcct
     1861 gtgcttcggg cagtggtcag cagttcggcg accttcgccc gagccgcttg cggcaggcta
     1921 tcggtcaggg ctttgccttg catgacggtc ggtggaaccc ccagaagctc ctcacatgcg
     1981 gggttcgccg cgagcacgca ttggctgtcc gcgtcaatca acaggacggc atcgttggct
     2041 acctggaaga ggagtcgata acgagtctca gcctgacggc ggttccagta ctggcgctcc
     2101 atatcctgct gcgcctcgac gaagcgctgc tggatggccg cgattgcgcg caggtcgcga
     2161 ccaacggcaa gaaccggccc atcagcaccg aggcgaattg ccgaccaggt gacaggcagt
     2221 tcgtcaccga tctgcgacag gtggttaacc tcgcgccggc gcgccgtgag gccgctgctc
     2281 gcgacctcat ccatcaacga ctggatcttt ggccgggtgc ttgcgctgac ggtgtcaatc
     2341 cagtgctgac ccacccattg attgcaaccg tgctcctgca cggccgcccc ggcggccacg
     2401 gtggcgatga caccgtctgc gtcgatgacc agcgctatat cgctagcaac gtgggcaatc
     2461 gtcgcggcca gttcacgggc cagaggcgac agtggactga gatctagttc gagcgtggtg
     2521 gacactggct ggggtctcgg tcacgtggcg ataccacagg gagcgttacg cgcaccgggt
     2581 ggcgacgtct gaacttatca caaacactcc gatcgctggc atcacttgca gtgccgctcg
     2641 aaagatgccc caagtctgcg ggcacctcgt cctccgggca gaaggagtcc gaaatgggaa
     2701 gaagcaagcc gccatttcgg gccgcattca ggtgaggagt cgatggccgg gtgagataac
     2761 gggccgccga gtgccaggaa gtcgctcgag tgcgacgaca ggtgcgcgag ctgtgttgac
     2821 gatcacttga agttgcgccg cttacggggt gctctcgaca gccgccacga attccaaggc
     2881 gattgggatc tgacgcggcc gcctggagag gagccgtcgg tttgcccagc acggaaattc
     2941 ttgggggcgc aaccgaggag cccgaactgg cgacttccgg ggtggggcaa ccgagcttcc
     3001 caagcgcact ttcttcgcgg tttttttgat ggatagaaat aaaaacgggc cgcgaaggcc
     3061 cgtgtttcat agctacctgg cggagagagc gggattcgaa cccgcggggg gttattagcc
     3121 cccgcacgct ttccaggcgt gtgacttaaa ccgctcatcc atctctccgg agcccggtag
     3181 tctatccgac gggcttcagt gcgtgggcgc tcaatgtctc cagcggcacg tggtcgagcg
     3241 ccttgcggtg cgtgctttcc agcaccaccg gcggggcgac accggccttc aacgcttcca
     3301 gccatcgcgt gacgcacagg caccagcggt cacccggctt caggccggcg aaccggaact
     3361 ccgggtgagg ggtcgtcagg tcgttgcccc gttcggccga gtaagccagg aactccttcg
     3421 tgaccttggc acacaccaca tgcgtgccgg tgtcgtcggc gcgggtgttg cagcaaccgt
     3481 cgcggaagaa gccggtcagt ggatcgtagg aacaagggat cagttcggtg cccagcacgt
     3541 tggcggccac ggtcagtttg ctccgggagg cttggtcttc agcagcgtca tggcggtgcc
     3601 gatcaaggcc gacacctcgc tcatgttgcc cggcacgatc atcgtgttgt tcttctgtgc
     3661 cagctgcgca taggcctcga cggctttctc ggcgaccttc agctggacgg cctgttcacc
     3721 gcccggcgag cggatcgctt cggcgatctt tcgaatggcc tcggcggtgg cgtcggccac
     3781 cgcgatgatg gctgaagcct cgccctgcgc attgttgatc tcggcctggc gctggccttc
     3841 ggagcgggcg atgaacgctt cgcgctcgcc ggtggcgatg ttgatctgct cctgcttgcg
     3901 gccttcggaa gccgcgatca gcgcgcgctt ctcgcgctcg gcggtgatct gcgcctgcat
     3961 cgaacgcagg atctcggccg gcggggtgag gtccttgatc tcgtagcgca gcaccttcac
     4021 gccccagttg gcggcggcct cgtcgagcgc gttgaccacc gaggcgttga tggtgtcgcg
     4081 ttcctcgaag gtcttgtcga gttccatctt gccgatcacc gagcgcagca gcgtctgcgc
     4141 cagctgcgtg atcgcgacga tgtagttgct cgatccgtag ctggcgcgca tcgggtcggt
     4201 gacctggaaa tagatgatcc cgtcgacctg caactgggtg ttgtcacgcg tgatgcagat
     4261 ctggctgggc acgtcgagcg gcacttcctt cagcgagtgc ttgtaggcca cgcgttcgac
     4321 gaacgggacg acgaacttca gtcccggagt cagtgtgcgg tcgtacttgc ccagccgctc
     4381 tacgacccag gcgttctgct ggggcacgat cttgaaggtg cggacgatga agatgatcgc
     4441 gatgacagcg atgacgacgg cgaattccat ggctggcgtt ccttgagtga gcgagtgggg
     4501 ccgaactaca gggccgagtg cggtgccgtg gtggccgggt ccgctggtga tggcgatgcc
     4561 gatgacgcgg gggccacgac gagccagttg ccctcgacgg cggtgacgcg gtgcatgccc
     4621 gcagccgctg cacagcccgg ctgcagacgg gccgtccagg cggtgccccg ataggaaacg
     4681 cgagcggtga ggtcgggcgc ccattcgacg acttgcacgg tctcgccgat gtcgaggttg
     4741 acatcgcggt tttcgcgggc gggcaggctg tgcggatgtt gcatgcgctt ccagtgccac
     4801 agcgctgtcg ctccgctcgc aaccactgcc gcaacagtga cctgcatcga accacccagc
     4861 ccgaggtgtg ccgccagtgc accacaggcg aggccaagcg cgatcatcag caggtagaag
     4921 gttccggtgg cgagctcgac cgccaccgcc agcccggccg ccacccacca gaacgtggcg
     4981 tgtgcaaagt ccatgacacc ctcgctattt caggtccgta tcgaccgggc gcagtgtagt
     5041 tcccgtgcga tttctcgacg tggggcgcag ttgggtcgag cgcggatcaa tgggcgtcga
     5101 gccgtcacgc cttcggccta aacttcgcgc tctttccacg ctgcccaccg ggactgccat
     5161 gctgcgcttt cgctttccga tcgtgatcat cgacgaggac taccgctcgg aaaacacctc
     5221 cggtctgggc atccgtgcgc tggcgcaggc catcgagaag gaaggcttcg aagtgctggg
     5281 tgccaccagc tacggcgacc tcagccagtt cgcgcagcag cagagccgcg ccggtgcctt
     5341 catcctgtcg atcgacgacg aggagttcac gcccggcccc gagctcgatc cggcggtgct
     5401 gaacctgcgc aagttcatcg aggaaatccg gttcaagaac gccgacatcc cgatctacat
     5461 ctacggcgag acccgcacct cgcagcacct gccgaacgac gtgctgcgcg agctgcacgg
     5521 tttcatccac atgttcgagg acacgccgga gttcgtcgcg cgtcacatca tccgcgaggc
     5581 caagacctac ctggacgggc tggcgccgcc gttcttcaag gcactgatgg actacgcgca
     5641 ggacggctct tactcgtggc actgccccgg tcacagtggc ggcgtggcgt tcctgaagag
     5701 cccggtcggc cagatgttcc accagttctt cggcgagaac atgctgcgtg ccgacgtctg
     5761 caacgcagtc gaggagctcg gccaactgct cgaccacaac ggcgccatcg gcgcgtccga
     5821 gcgcaacgcc gcgcgcatct tcaatgccga tcactgcttc ttcgtcacca acggcaccag
     5881 cacctccaac aagatggtgt ggcaccacac ggtggcgccg ggcgacgtgg tggtggtcga
     5941 c
//
LOCUS       DAOORV010000197         5941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16244_length_5941_cov_8.331634, whole genome shotgun sequence.
ACCESSION   DAOORV010000197 DAOORV010000000
VERSION     DAOORV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16244_length_5941_cov_8.331634"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKV56_16825"
     CDS             <1..324
                     /locus_tag="PKV56_16825"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579400.1"
                     /note="Activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOX69524.1"
                     /translation="LKIVDRKKDMIIVSGFNVNPNEVEAVVTGCPGVAECACVGAPDE
                     KSGEAVKLFVVKAPASALTEADVIAYCRQQLTGYKVPKFVRLVDALPKSTVGKILRRE
                     LRDVA"
     gene            342..818
                     /locus_tag="PKV56_16830"
     CDS             342..818
                     /locus_tag="PKV56_16830"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HOX69525.1"
                     /translation="MVEKRIPATVDKAYALLIDPKWLEARCLALGELTASCKTKKSPG
                     VVVSMKRRVRRELNAIISKVLNPESDFELEEQWSAEGKGYKGRLTLQIVGKPITVTGD
                     FELLPDGKGSIYRIQHQAKVKVPLIGGVVEKFVLGQTEQGCANELDYLADFVKKNK"
     gene            815..2476
                     /locus_tag="PKV56_16835"
     CDS             815..2476
                     /locus_tag="PKV56_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOX69526.1"
                     /translation="MKPQPCTMDEGDFDFDWLVIGSGFGGSVSALRLTEKGYRVAVLE
                     CGRRYRDEDFAESTWQLGRFLWAPLLGLKGILRLAPFKDVFAASGAGVGGGSIVYANT
                     LYRAKPAFFSSAQWGALAQWDQVLQPHYDTAERMLGVQTVPFDSAAQQLIREMARHFR
                     TEDSFTRTPCGVYFGEPGKTVADPYFGGDGPARTGCTRCGACMVGCRVGAKNTLLKNY
                     LWFAEKCGVQILPEHQVSGVAPLGAADGSDGYRVTTQRPGAWFAKGTRTFTARGVVFA
                     AGSLGTNLLLANCKHGGSLPRISDRLGDLVRTNSESILTVRLPEDRKTWNDVAISCSI
                     HVDHDTHIEFVNYGRNADFMSLLYTVLVGKGSRLTRPLLWLGSIARHPLQWLKTLWPA
                     GWSQRMVMLLVMQALDNAIALRARKRWFGRGYRLSTEQDRDKPNPTFIAAGNAAAAWL
                     AGHTGGIAQSNVLEALGNVPTTAHLLGGAVIGADASCGVIDQHLRVFGYRNLLVCDGA
                     AMPANPGVNPSLTITALAEHAMSTIAPAARATPACAGAESVGATA"
     gene            2473..3948
                     /locus_tag="PKV56_16840"
     CDS             2473..3948
                     /locus_tag="PKV56_16840"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HOX69527.1"
                     /translation="MSTSPKPHELPVQSGDDEFARMRRLFLRQRQAFEAAPYPELALR
                     KAKLRKLIDALRRYQDDIVVAVNADFGVRAGAETKLVEVMGPILEARHALSHMGRWMK
                     PRRRSTELLFLTNRAWVAYQPKGVVGIIGTWNFPLYLTLGPLVAALAAGNRAMIKMSE
                     FSPRCTALLTSMLAECFDEDEVAVCGGDIGAAQAFNALPFNHLVFTGSPRVGRQVMRA
                     AAEHLTPVTLELGGKSPAIVGPDADLRESALRIAHGKAFNAGQICVAPDYALVPRGKA
                     EAFAAAVADAFTRLYPTVSGNAEYTSIITERHAVRLRELLADAQAKGATLLRCGADAG
                     SGRQMPLTVVTRVEERMQLMQEELFGPILPVLEYDSLDDAMALVRRGERPLSLYGLGL
                     SAADQARVLKETHAGGVTFNDWGWHVFQHDLPFGGIGNSGMGTYHGEEGFRELSHAKG
                     VFKRHRWFPVGLFYPPYGNLVQRLAMKFFIGSAPTPTRPPR"
     gene            3997..>5941
                     /locus_tag="PKV56_16845"
     CDS             3997..>5941
                     /locus_tag="PKV56_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOX69528.1"
                     /translation="MMKTTHRPAAIALLAVAALLASCGKNPGVGGAAGDAMPATVQAN
                     EQVARELRLDEPQGFEDARRGFIARPEGKILAVDGSTVLIDFDAFKFVDGKAPATVNP
                     SLWRHAMLNAQAGLFKVTDGIWQVRGFDIANMTLIEGKTGFIVVDTLTARESAAAALA
                     FARKHLGNKPVSAIVFTHSHADHFGGALGVATAKEVAERKIPVVAPEGFMAEATSENI
                     LVGTAMARRSMYQFGKNLVPSAKGIVDTGLGKNVAYGAIGILAPTQLITQPTQELMLD
                     GVRFVFHNVPGAEAPAELTFSIPEKKAYCGAENLAQTMHNLLPVRGAKVRDALRWAEY
                     MQQALDQLGDTEVYFGQHHWPIWGNVRIADFITKHRDIYKYTHDQTLRLANAGHTPRE
                     IAEMVKLPKSLQSHFGARGYYGDLRHNVKAVYQLYLGAYDGNPAHLNPHPPQESAKRY
                     LELLGGVDKAVAAGQAAFDKGDFRWAAELLNHAVFGAPDHKAAKELLARTYDQLGYMA
                     EAATWRNSYLTAAAELREGPPKKGVDRSFLIDMLYETPVERFLEAMAAGLDGPAADGK
                     NLKVNLVLTDTKESFVLWIENAVLHHKKAAPAADANATLTLTKPIFIKMMAGTAGIKD
                     TLMSDDLKIDGSKIDLVRFFTLID"
BASE COUNT         1062 a         1980 c         1970 g          929 t
ORIGIN      
        1 ctgaagatcg ttgaccgcaa gaaggacatg atcatcgtct cgggcttcaa cgtcaatccg
       61 aacgaggtcg aggccgtggt cactggctgc ccgggcgtgg ccgagtgcgc ctgcgtcggt
      121 gcgcccgacg agaagtcggg cgaggccgtg aagctctttg tcgtgaaggc gcccgcctca
      181 gcgctgaccg aggccgatgt cattgcctac tgccgccagc agttgaccgg ctacaaggtg
      241 cccaagttcg tgcgcctggt cgatgcgctg cccaagtcga cggtgggcaa gatcctgcgc
      301 cgcgaactgc gcgacgtggc ctgaagacaa ggagtcccat catggttgag aagaggatcc
      361 cggccacggt cgacaaggcc tacgcattgc tgatcgaccc gaagtggctc gaagcccgtt
      421 gcctggcgct gggggagctc acggcgagct gcaagaccaa gaaatcacct ggcgtggtgg
      481 tgtcgatgaa acgccgggtc aggcgtgaac tcaatgcgat catctcgaag gtgctgaacc
      541 ccgaaagcga cttcgagctg gaagagcagt ggtcggccga aggcaagggc tacaagggcc
      601 gtttgacact gcagatcgtc ggcaagccga tcaccgtgac gggcgacttc gagctgctgc
      661 ccgacggcaa aggcagcatc taccgcatcc agcatcaagc caaggtcaag gtacctttga
      721 tcggcggtgt ggtcgagaag ttcgtgctcg gccagaccga gcagggctgc gccaacgaac
      781 tggactacct ggcggacttc gtgaagaaga acaagtgaaa ccgcagccct gcaccatgga
      841 cgaaggcgac ttcgacttcg actggctggt gatcggatcc ggcttcggtg gcagtgtctc
      901 ggcgctgcgc ctgaccgaga agggctaccg cgtcgccgtg ctcgaatgcg ggcgccgcta
      961 ccgcgacgag gacttcgccg agtcgacctg gcagctcggc cgcttcctct gggcgccgct
     1021 gctcggcctg aagggcatcc tgcgcctggc gccgttcaag gatgtgttcg cagccagcgg
     1081 cgcgggtgtg ggtggcggca gcatcgtcta cgccaacacc ctgtaccggg ccaagccggc
     1141 gttcttcagc agcgcgcagt ggggcgcgct ggcgcaatgg gaccaggtgc tgcagccgca
     1201 ctacgacacc gccgagcgca tgctgggcgt gcagaccgtg ccttttgact cggccgcgca
     1261 gcaattgatt cgcgagatgg cgcgtcactt ccgcaccgaa gattcgttca cccgcacgcc
     1321 ctgtggcgtg tacttcggcg agccaggcaa aacggtggcc gacccttact tcggcggcga
     1381 cggccccgcg cgcaccggct gcacacgctg cggcgcctgc atggtgggct gccgcgttgg
     1441 cgccaagaac acgctgctca aaaactacct ctggttcgcc gagaagtgcg gcgtgcagat
     1501 cctgcccgag caccaggtca gcggcgtggc gccgctgggt gcagccgacg gcagcgacgg
     1561 ctaccgcgtc acgacccagc gccccggcgc ctggttcgcc aaagggacca ggaccttcac
     1621 cgcgcgaggc gtggtcttcg cggccggatc gctgggcacc aacctgctgc tggccaactg
     1681 caagcatggc ggctcgctgc cgcgcatcag cgaccgcctg ggagatctgg tgcgcaccaa
     1741 cagcgagtcg atcctgacgg tgcgcctgcc tgaggaccgc aagacctgga atgacgtggc
     1801 catcagctgc agcatccacg ttgaccacga cacccacatc gagttcgtca actacggccg
     1861 caacgctgac ttcatgtcgc tgctgtacac cgtgctggtc ggcaagggca gtcggctgac
     1921 gcggccgctg ctgtggttgg gcagcatcgc gcgccacccg ctgcaatggt tgaagacgct
     1981 gtggccggcc ggctggagcc agcgcatggt gatgctgctg gtgatgcagg cgctggacaa
     2041 cgccatcgcg ctgcgcgcgc gcaagcgctg gttcggccgc ggctaccggc tcagcacgga
     2101 gcaggaccgc gacaagccca acccgacctt catagccgcc ggaaacgccg ccgcggcctg
     2161 gctggccggg cacacgggtg gcatcgcgca gagcaacgtg ctcgaggcct tgggcaacgt
     2221 gccgaccacg gcgcacctgc tgggcggcgc ggtgatcggc gccgacgcca gctgtggcgt
     2281 gatcgatcag cacctgcgcg tcttcggcta ccgcaacctg ctggtctgcg atggtgccgc
     2341 gatgccagcc aaccccggtg tcaacccttc gctgacaatc accgcgttgg ccgaacacgc
     2401 gatgtcgacg atcgccccgg ccgcacgggc cacgccagcc tgcgccggcg ccgaatccgt
     2461 tggggccacg gcatgagcac ctcgccgaaa ccgcacgaat tgcccgttca gtctggcgac
     2521 gacgagttcg cgcgcatgcg gcgactgttc ctgcgtcagc ggcaagcgtt cgaggccgcg
     2581 ccctatcccg agctggcgct gcgcaaggcc aagctgcgca agctgatcga tgcgctgcgc
     2641 cggtaccagg acgacatcgt cgtggcggtg aatgccgact tcggcgtgcg cgccggtgcc
     2701 gagaccaagc tggtcgaggt gatggggccg atcctcgagg cacggcatgc gctgtcgcac
     2761 atgggccgct ggatgaagcc gcggcgtcgc agcaccgagt tgctgttcct gaccaaccgt
     2821 gcttgggtcg cctaccagcc caagggcgtg gtcggcatca tcggcacctg gaatttcccg
     2881 ctgtacctga ccctgggccc gctggtggcg gcgctggccg cgggcaaccg cgccatgatc
     2941 aagatgtcag agttctcgcc gcgctgcacg gcgctgctga cttcgatgct ggccgagtgc
     3001 tttgatgaag acgaggtcgc tgtgtgcggt ggcgacatcg gcgcggccca ggccttcaat
     3061 gccctgccgt tcaaccacct ggtgttcacg ggctcgcctc gtgtgggtcg ccaagtgatg
     3121 cgggctgcgg ccgagcacct cacaccggta acgctggagc tgggtggcaa gtcgcccgcc
     3181 atcgtcggcc ccgatgccga cctgcgtgag tcggccctgc gcatcgccca tggcaaggcc
     3241 ttcaatgctg ggcagatttg cgtcgcaccc gactacgcgc tggtgccgcg cggcaaggcc
     3301 gaagccttcg cggctgccgt ggccgatgcc ttcacccggc tctaccccac ggtgagtggc
     3361 aacgccgagt acaccagcat catcaccgag cgccatgccg tgcgcttgcg cgaactgctg
     3421 gccgatgcgc aggccaaagg ggcgacattg ctgcgctgcg gcgccgacgc tggcagcggc
     3481 cggcagatgc cgctgaccgt ggtcacgcgc gtcgaagagc gcatgcaatt gatgcaggag
     3541 gagctcttcg ggccgatcct gccggtgctc gaatacgaca gtctggacga cgcgatggcg
     3601 ctggtgcggc gcggcgagcg gccgctgtcg ctgtatggcc tgggcctgag cgctgctgac
     3661 caggcgcgtg tgctcaagga aacacatgcc ggcggcgtga ccttcaacga ctggggctgg
     3721 catgtcttcc agcacgacct gccttttggc ggcatcggca attcgggcat gggcacctac
     3781 cacggcgagg aaggctttcg cgagctctcg catgcgaagg gcgtgttcaa aaggcaccgc
     3841 tggttccccg tcgggctctt ctatcccccc tacggcaacc tcgtgcaacg cttggcgatg
     3901 aaattcttca tcggcagtgc accgacacca acccggccgc ctcgctgaag cgggcaaccg
     3961 gcccgagcga taaccacgaa tctcgaggag acgatgatga tgaagacaac acaccgcccc
     4021 gcggcgatcg cgttgctggc cgtcgcggcc ctgctggcat cctgcggcaa gaatcctggt
     4081 gttggcggcg cggctggcga cgccatgccg gccaccgtgc aggccaatga gcaggtggcg
     4141 cgggaacttc ggctcgacga accgcagggc ttcgaggacg cccggcgcgg cttcatcgcc
     4201 agacccgagg gcaagatcct cgccgtcgac ggcagcaccg tgctgatcga cttcgacgcc
     4261 ttcaagttcg ttgacggcaa ggcgccggcc accgtcaacc cgagcctgtg gcgccacgcg
     4321 atgctgaacg cgcaggcggg gctgttcaag gtcaccgacg gaatttggca ggtgcgcggc
     4381 ttcgacatcg ccaacatgac gctgatcgaa ggcaagaccg gcttcatcgt cgtggacaca
     4441 ctgaccgcgc gcgagagcgc cgcggcggcg ctggcctttg cgcgcaagca cctgggcaac
     4501 aagccggtct cggccatcgt cttcacgcac agtcacgccg accacttcgg tggcgcgctc
     4561 ggagtggcca cggccaagga ggtggccgag cgcaagatcc cggtggtggc gcccgagggc
     4621 ttcatggccg aggctacgag cgagaacatc ctggtcggta ccgccatggc ccggcggtcg
     4681 atgtaccagt tcggcaagaa cctcgtgcca tcggccaagg gcatcgtcga cacgggtctc
     4741 ggcaagaacg tggcctatgg tgccatcggc attctggcgc ccacgcagct gatcacgcaa
     4801 cccacgcagg aactgatgct ggacggcgtg cgctttgtct tccacaacgt gccgggtgcc
     4861 gaagcgccgg ccgagctcac cttctccatc cccgagaaga aggcctactg cggcgcggag
     4921 aatctcgcgc agaccatgca caacctgctg ccggtgcgcg gcgccaaggt gcgcgacgcg
     4981 ctgcgctggg ccgaatacat gcagcaggcg ctggaccagc tgggcgacac cgaggtctac
     5041 ttcggccagc accactggcc gatctggggc aacgtgcgca ttgccgattt catcaccaag
     5101 catcgcgaca tctacaagta cacgcacgac cagacgctgc gcctggccaa cgccggccac
     5161 acgccgcgcg agatcgccga gatggtcaaa ctgccgaagt cactgcagtc gcatttcgga
     5221 gcccgcggct actacggcga cctgcgccac aacgtgaagg cggtgtacca gctctacctc
     5281 ggcgcctacg acggcaatcc ggcccacctg aacccgcatc cgccgcagga atccgcgaag
     5341 cgctacctgg aactgctggg cggcgtcgac aaggccgtgg ccgcaggcca ggccgcgttc
     5401 gacaaaggcg acttccgctg ggctgccgag ctgctgaacc acgccgtgtt cggcgccccc
     5461 gaccacaagg cggccaaaga gctgctggcc cgcacctacg accagctggg ctacatggcc
     5521 gaggcggcca cctggcgcaa cagctacctc accgccgcag ccgaactgcg cgaaggccca
     5581 ccgaagaagg gtgtggatcg ctcgttcctg atcgacatgc tgtacgagac cccggtggag
     5641 cgcttcctcg aagccatggc ggctggcctg gacggcccgg ccgcggacgg caagaacctg
     5701 aaggtcaacc tggtgctgac cgacaccaag gagtccttcg tgctgtggat cgaaaacgcg
     5761 gtgctgcacc acaagaaagc cgcgccggcg gccgatgcca acgcgacgct gacgctgacc
     5821 aagccgatct tcatcaagat gatggccggc accgctggca tcaaggacac gctgatgagc
     5881 gacgatctca agatcgacgg cagcaagatc gacctggtgc gcttcttcac gctgatcgac
     5941 a
//
LOCUS       DAOORV010000198         5933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16273_length_5933_cov_5.636951, whole genome shotgun sequence.
ACCESSION   DAOORV010000198 DAOORV010000000
VERSION     DAOORV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16273_length_5933_cov_5.636951"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKV56_16850"
     CDS             <1..280
                     /locus_tag="PKV56_16850"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOX69529.1"
                     /translation="KTVEQNFELLRRQRELLDLGHPLLAGWSRKSSIGAVTGREAGDR
                     VAGSVAAALAAVLNGASIVRVHDVAATVDALKVWRAAGLPAAASRKSQ"
     gene            294..1625
                     /gene="glmM"
                     /locus_tag="PKV56_16855"
     CDS             294..1625
                     /gene="glmM"
                     /locus_tag="PKV56_16855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOX69530.1"
                     /translation="MSRSYFGTDGIRGAVGQSPITPDFMLRLGHSVGRVLKASESKPT
                     VLIGKDTRISGYMIESSLEAGFASAGVDVLLTGPLPTPGVAYMTRALRLSLGVVISAS
                     HNPYGDNGIKFFSARGEKLPDEWEQQVEAALEQPPQWVTSADLGKARKLEDARGRYIE
                     FCKSTFGSELSLKGMKIVVDAANGAAYNVAPSVFHELGAEVIAIGCSPDGTNINAGFG
                     ATSPAALVRAVQEHHADCGVALDGDADRLQFVDAAGRLYNGDELLYVMVADRLAQGQA
                     VPGVVGTLMTNLAVEVALRQRGLDFVRARVGDRYVLEELLARGWTLGGEGSGHLLSLD
                     RHTTGDGIISALQILQSVRRSGIPLAEQLRSVQLCPQVMINIRLQDGLDWKTHAPLHR
                     TQREIEKDLGSSGRVLIRASGTEPVLRIMVEASDEGTARSCAERLADSLKV"
     gene            complement(1626..2111)
                     /locus_tag="PKV56_16860"
     CDS             complement(1626..2111)
                     /locus_tag="PKV56_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOX69531.1"
                     /translation="MQIKKMLMVSALTMLIATGCATAPTPITISETAAATPQLSTLTK
                     LLNDAGLTETLKGPGPYTVFAPTDDAFKAVPAKTLDALAKDKALLTSVLTYHVLPGAV
                     TSAAVVNGPVKTVQGAPVAIYKSGTFVTVEEAVITTPDIAASNGVVHIVDKVLMPPVK
                     K"
     gene            2370..3407
                     /gene="pstS"
                     /locus_tag="PKV56_16865"
     CDS             2370..3407
                     /gene="pstS"
                     /locus_tag="PKV56_16865"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOX69532.1"
                     /translation="MNFTLNRIVAISMAFFAASGTSLAQDATGAGATFPAPIYAKWAD
                     AYNKATGAKVNYQSVGSGAGLKQIRSKTVDFGASDMPLKDDELAKDGLVQFPTVIGGV
                     VPVVNIKGIAPGQIKLTGAVLGEIYLGKITKWNDAALTALNPGVPLPDAAISVVRRAD
                     GSGTTFVFTNYLSKVNAEWKAKAGEGTAVNWPTGAGGKGNEGVAAFVTRLPNSIGYVE
                     YAYVKQNKMTYTQLQNQAGTFVSPDADNFQAAAAGADWSKTFYQILTDQPGKASWPIT
                     GATFIMMHKSQDKPGQATNSLKFFDWAFVTGDKMAAELEYVPLPDAVKALIRKQWNEI
                     KDPAGKVIAYK"
     gene            3534..4511
                     /gene="pstC"
                     /locus_tag="PKV56_16870"
     CDS             3534..4511
                     /gene="pstC"
                     /locus_tag="PKV56_16870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HOX69533.1"
                     /translation="MERSDPMGTPPTARRSSPWVDKVFSGLAHGAALLTLALLFGILG
                     SLVVGAWPAISEYGLSFLWRSEWDPVQDSYGGLVMIYGTLMTSFIALLIAVPVSFGIA
                     LFLTELSPNWLRRPLGVAVELLAAVPSIVYGMWGLLVFSPVLSTYIQQPLQKLLVDVP
                     ILRDLVSGAPVGIGILSAGIILAIMIIPFIASVMRDVFEVTPAMLKESAYALGATTWE
                     VVFKIVLPYTKTGVIGGIMLGLGRAIGETMAVTFVIGNFNQLNSLSLFEAANSITSAL
                     ANEFAEASEGLHQASLIYLGLVLFFITFVVLSLSKILLARLQKNEGSRS"
     gene            4508..5377
                     /gene="pstA"
                     /locus_tag="PKV56_16875"
     CDS             4508..5377
                     /gene="pstA"
                     /locus_tag="PKV56_16875"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOX69534.1"
                     /translation="MSTSEALHASRLAKHTHRKRVNAVAMVLCLSAMAFGLFWLAWIL
                     IETVRLGIGGLTLSIFTEMTPPPQAETGGLLNAIFGSLVMVGLATALGTPIGVMAGVY
                     LAEYGQKTWLGTTTRFINDILLSAPSIVIGLFVYTVVVAPMKSFSGFAGVLALALIVI
                     PVVIRTTENMLSLIPAALREAAYALGTPKWKVILSITLKSARAGVITGVLLAVARIAG
                     ETAPLLFTALSNQFWSSSLGEPMASLPVTIFKFALSPYENWQKLAWAGVFLITLGVLL
                     LNILARVLFRNKS"
     gene            5396..>5933
                     /locus_tag="PKV56_16880"
     CDS             5396..>5933
                     /locus_tag="PKV56_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX69535.1"
                     /translation="MDKKVDVPVTEKIKISVRNLNFYYGKFHALKNINLELPEKKVTA
                     FIGPSGCGKSTLLRTFNRMFELYPEQRAEGEILLDGENILTSKKDVSLMRANVGMVFQ
                     KPTPFPMSIYDNIAFGVNLFETLPRVEMDERVEWALKKAALWNEVKDKLGQSGSGLSG
                     GQQQRLCIARGIAIKPEVL"
BASE COUNT         1076 a         1790 c         1883 g         1184 t
ORIGIN      
        1 caagacggtc gaacaaaatt tcgagttgtt gcggcgccag cgtgaactgc ttgatctcgg
       61 tcatccgttg cttgcggggt ggtcccgaaa atcgtcgatc ggcgcagtga ccggacgcga
      121 agccggggac cgcgttgccg gcagcgtggc ggcggccctg gctgcggttc tgaatggcgc
      181 cagcatcgtc cgtgtgcatg atgtcgcggc gacggtggat gccttgaaag tctggcgtgc
      241 tgcgggattg ccagcagccg catcgcggaa atcacagtga ggaggatgct ccgatgagca
      301 gatcgtattt cggtaccgac ggcatacgcg gtgcagtggg acagagcccg atcacaccag
      361 acttcatgct ccgcctgggg cattcagtgg gtcgcgttct gaaggcttcc gagtcgaagc
      421 ccacggtgct gatcggcaag gacacccgca tttcgggcta catgatcgag tcgtcactgg
      481 aggccggctt tgcgtcagcc ggcgtcgacg tgctgttgac cggaccgttg ccgacgcccg
      541 gagttgcgta catgacgcgg gcattgcgcc tcagtctcgg tgtggtgatc agcgcgtctc
      601 acaaccccta cggagacaac gggatcaagt tcttctcggc gcgcggcgag aagttgccgg
      661 acgaatggga gcaacaggtc gaggcggcgc tcgaacagcc gccgcagtgg gtgacttcag
      721 ctgatttggg caaggcgcgc aagctggaag acgcacgtgg ccgctacatc gaattctgca
      781 agagcacctt cggcagcgag ctgtcgctca aggggatgaa gatcgtcgtc gatgccgcca
      841 acggtgccgc ctacaacgtg gcgccgagcg tgttccatga attgggtgcc gaggtcatcg
      901 cgatcggctg cagccccgat ggcaccaaca tcaatgccgg attcggtgcg acgtcgccgg
      961 cggccctggt gcgcgcggtt caggagcatc acgctgactg cggcgtggcg ctggacggtg
     1021 atgccgatcg cctccagttc gtcgatgcgg ccggccggct ctacaacggc gacgagcttc
     1081 tttacgtgat ggtggccgac cgtctggccc agggtcaggc tgtgccgggt gtggtgggca
     1141 ccttgatgac caacctcgcc gtggaagtcg cgctgcgcca gcgcggcctg gacttcgtgc
     1201 gtgcgcgcgt cggcgaccgc tacgtgctgg aagagctgct ggcgcggggc tggaccctgg
     1261 gtggggaagg ttccgggcac ctgctgtcgc tggaccgtca cacgacgggc gatggcatca
     1321 tcagtgcgct tcagattctc cagtcggtca ggcgcagcgg aattcccttg gccgaacaac
     1381 tgcgatcggt gcaactgtgt ccgcaggtca tgatcaacat acgacttcag gacgggctgg
     1441 actggaagac acacgccccg ctccaccgaa cccagcgcga gatcgagaag gacctcggca
     1501 gttccggccg ggtcctgatc cgcgcctccg gcaccgagcc ggtcctgcgc atcatggtcg
     1561 aggccagcga cgaaggcacg gcgagaagct gtgccgagcg gctggccgac tcgctgaagg
     1621 tctgactact tcttgaccgg cggcatcagc accttgtcaa cgatgtgcac cacgccgtta
     1681 gacgccgcga tatcgggggt cgtgatgacg gcttcctcga ccgtgacaaa cgttcctgac
     1741 ttgtagatcg caaccggtgc gccctgcacg gtcttgaccg gtccgttcac gaccgcggca
     1801 gaagtcaccg cgccggggag cacgtgatac gtcaggactg acgtcagcaa ggccttgtcc
     1861 ttcgccagtg catccagggt cttcgcgggt accgccttga acgcgtcatc ggtcggagcg
     1921 aacacggtgt acgggccagg gcccttcagt gtttcggtca agccggcgtc attgagcagt
     1981 ttggtcaagg tcgacagctg cggcgtcgcc gctgcggttt cgctgatcgt gatcggtgtc
     2041 ggtgccgtgg cgcagccagt cgcaatcagc atggtcagcg cgctgaccat caacatcttc
     2101 ttaatttgca tggagagctc ctgtgaggtt ctgggtgagg cgccgcacgt gcgacacgcc
     2161 gccttgtttg tcgagcccgt ctaagttatg gagtatttgt gacaagtagg tgaaggcaat
     2221 atgacggttt tgataggctg gcttggctat cgcggatcag caacgtggat cggcgcactg
     2281 tcaccgtgtg tcatggcgat gtcatattcc atgaatagag tcagcctgac ttcattgacc
     2341 gcctggtgcg gatacagaaa ggctcgaaca tgaatttcac gctgaaccga atcgtcgcca
     2401 tctcgatggc cttcttcgct gcatccggca cctcgttggc acaggacgcc accggcgcgg
     2461 gcgcgacgtt tcctgcgccg atctatgcga agtgggctga tgcctacaac aaggccaccg
     2521 gtgccaaggt gaactatcaa tccgtcggat ctggcgccgg actgaagcag attcgcagca
     2581 agacggtcga tttcggcgcc tccgacatgc ccttgaagga cgacgaactg gccaaggacg
     2641 gcctggtcca gttcccgacc gtgatcggcg gcgtggtccc cgtggtcaat atcaagggca
     2701 ttgcgccggg ccagatcaag ttgaccgggg cggtgctcgg tgaaatctac ctgggcaaga
     2761 tcaccaagtg gaacgacgcg gcactgaccg ccctgaatcc gggtgttccc ctgcctgacg
     2821 cagccatttc ggttgttcgc cgtgccgacg gttcgggaac cacctttgtc ttcacgaatt
     2881 acctctcgaa ggtcaatgcc gaatggaagg ccaaggcagg tgagggtacc gccgtcaact
     2941 ggcccacggg tgctggcggc aagggcaatg agggcgttgc cgccttcgtc acgcgcctgc
     3001 cgaattcgat cggctatgtc gaatacgcgt acgtcaagca gaacaagatg acctacacgc
     3061 aactgcagaa ccaggcagga accttcgtca gtcccgatgc cgacaatttc caggccgctg
     3121 cggcgggtgc cgactggtcc aagaccttct accagatcct gacggatcag ccgggcaagg
     3181 cctcctggcc catcaccggt gccacgttca tcatgatgca caagtcccag gacaagccag
     3241 gccaggccac gaactcgctg aagttctttg attgggcctt tgtcacgggc gacaagatgg
     3301 ctgctgaact ggaatacgtc ccgctgccgg acgctgtgaa ggccttgatc cgcaagcagt
     3361 ggaacgagat caaggacccg gccggcaagg tgatcgccta caagtgatcg gttgattcga
     3421 atctctctat tcgattgagg ggcggggcgc agcggcgctg gccccgttta tccccaccga
     3481 tcggagaaat cgtggccgct acacttcctg cctctcccta cgacaagaac aacatggaac
     3541 gatccgaccc gatgggcaca cccccgacgg cgcgtcgatc gtcgccctgg gtcgacaagg
     3601 tgttttccgg tctggcccac ggggcggcgc tgctgacctt ggcgctgctc tttgggatcc
     3661 tcggttcgct ggttgtcgga gcctggcctg cgatctcgga atacggcctg agtttcctgt
     3721 ggcgcagcga gtgggatccg gtccaggaca gctacggcgg tctggtgatg atctatggca
     3781 cgctgatgac gtcgttcatc gcgctgctga tcgcggtgcc tgtcagcttc ggaattgcgc
     3841 tgttcctcac cgaactgtcg cccaactggt tgcgccggcc gctgggcgtc gcggttgaac
     3901 tgctcgctgc cgtgccatcg atcgtctacg gcatgtgggg cctgctggta ttcagcccgg
     3961 ttctttcgac ctatatccag caaccgctgc agaaacttct ggtcgatgtc ccgatcttgc
     4021 gcgacctggt ttcgggcgcg ccagtcggta tcggcatcct gtccgcgggc atcatcctgg
     4081 cgatcatgat catcccgttc atcgcttcgg tgatgcgcga tgtattcgag gtcacgccgg
     4141 cgatgctcaa ggagtcggcc tatgcgctgg gcgcgacgac atgggaagtc gtgttcaaga
     4201 tcgtactgcc gtacaccaag accggcgtca tcggcgggat catgctcggg ctcgggcgcg
     4261 ccatcggcga aaccatggcg gtgacctttg tcatcggcaa cttcaatcag ctcaacagcc
     4321 tctccctgtt cgaggccgcc aacagcatca cctcggcatt ggccaacgag ttcgctgaag
     4381 ccagcgaagg attgcaccag gcttccctga tctacctggg actggtgctg ttcttcatca
     4441 ctttcgtcgt gctttccttg tccaagatcc tgctggcgcg cctgcagaag aacgaaggga
     4501 gccgctcatg agcacctcgg aagccctgca tgcttcgcgg ctcgccaagc acactcatcg
     4561 caagcgggtg aacgccgtgg ccatggtgct gtgcctgagc gcgatggcat tcggtctgtt
     4621 ctggctggcc tggatcctga tcgagacggt tcggctgggc atcggtggct tgacgctgtc
     4681 gatcttcacc gagatgactc ccccacctca ggccgagacc ggaggcctgc tgaacgcgat
     4741 cttcggctcg ctggtgatgg tcgggttggc cacggcgctt ggcacgccga ttggcgtgat
     4801 ggctggtgtc tacctggccg aatacggcca gaagacgtgg ctcggcacga ccacgcggtt
     4861 catcaacgac atcctgttgt cggcaccttc gatcgtcatc ggtttgttcg tctacaccgt
     4921 cgtggtggca ccgatgaagt cgttctccgg gttcgccgga gtgctggccc tggcactgat
     4981 cgtcattccc gtggtcattc gcaccaccga gaacatgctg tcgctgattc cggccgcact
     5041 gcgcgaggcg gcctatgcct tgggcacacc caagtggaag gtcatcctca gcatcacgct
     5101 caagtcggcg cgggcgggcg tgatcaccgg ggtgctgctc gcggtggccc gcatcgccgg
     5161 tgaaaccgcg ccgttgctgt tcaccgcgct ctccaaccag ttctggtcgt ccagcctggg
     5221 tgagccgatg gccagcctgc cggtcacgat tttcaagttc gcgctcagcc cctacgagaa
     5281 ctggcagaag ctggcctggg ccggagtgtt cctgatcacg ctgggggttc tgctgctgaa
     5341 catccttgcg cgtgttctct tccgcaacaa gagctgacgg ccggaggccc ccaacatgga
     5401 caagaaagtc gacgtcccgg tgaccgagaa gatcaagatc tcggtgcgca acctgaactt
     5461 ctattacgga aaattccacg cgctgaagaa catcaatctc gaattgccag agaagaaggt
     5521 gaccgccttc atcggaccat ccggttgcgg caagtcgacg ctcttgcgca ccttcaaccg
     5581 catgttcgag ctgtacccgg agcagcgtgc cgaaggcgaa atcctgctgg atggcgaaaa
     5641 catcctgacc tccaagaagg acgtttcgct gatgcgtgcc aacgtcggca tggtctttca
     5701 gaagcccacg ccgttcccga tgtcgatcta cgacaacatc gcgttcggcg tgaatctttt
     5761 cgagaccttg ccgcgcgtcg agatggacga gcgagtcgag tgggcgctca agaaggcggc
     5821 gctgtggaac gaggtcaagg acaagctcgg ccagagcggc tccgggctgt ccggcggcca
     5881 gcagcaacgc ctgtgcatcg cgcgcggcat cgcgatcaag cccgaggtgc tgc
//
LOCUS       DAOORV010000199         5926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16296_length_5926_cov_9.202691, whole genome shotgun sequence.
ACCESSION   DAOORV010000199 DAOORV010000000
VERSION     DAOORV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16296_length_5926_cov_9.202691"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKV56_16885"
     CDS             complement(<1..199)
                     /locus_tag="PKV56_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOX69536.1"
                     /translation="MAKRDYYETLGVAKNASEEDIKKAYRKLAMKHHPDRNQGDDAKK
                     AEEKFKEAKEAYEMLSDTQKRA"
     gene            complement(330..2279)
                     /gene="dnaK"
                     /locus_tag="PKV56_16890"
     CDS             complement(330..2279)
                     /gene="dnaK"
                     /locus_tag="PKV56_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOX69537.1"
                     /translation="MAKIIGIDLGTTNSCVSIMEGNTTKVIENSEGARTTPSIVAYQE
                     SGEILVGASAKRQAVTNPKNTLYAVKRLIGRKFTEKEVQKDIDLMPYKITAADNGDAW
                     IEVRGKKLAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKHGKGDRKIAVYDLGGGTFDISIIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIDYIVSEFKKEQGVDLSKDVLALQRLKEAAEKAKIELSN
                     ATQTDINLPYVTADASGPKHLNIKLTRAKLESLVEELIERTIAPCRTAIKDAGVAVSA
                     IDDVILVGGMTRMPKVQDKVKEFFGKEPRKDVNPDEAVAVGAAIQGQVLAGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMIKKNTTIPTKFAQTFSTADDNQPAVTIKVYQGEREMAS
                     GNKSLGEFNLEGIAPAARGTPQIEVSFDIDANGILHVGAKDKGTGKENKITIKANSGL
                     TEAEIQQMVKDAELNAAEDKKKLGLIQARNSADAMIHSVKKSLGEHGDKLEAGEKEKI
                     EAALKDAEESLTSEDQATIEAKTEALMTASQKLGEKVYADAQAAAGAAEAASAGGAGA
                     SAAPSDKPADDNVVDAEFTEVKDKK"
     gene            complement(2391..2921)
                     /gene="grpE"
                     /locus_tag="PKV56_16895"
     CDS             complement(2391..2921)
                     /gene="grpE"
                     /locus_tag="PKV56_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOX69538.1"
                     /translation="MSQTESQTPSPSDAAGTAAPGSAASDPLSKLAELEAKHAEVSDA
                     YLRAKAEAENIRRRAEEEISKARKFAVEGFAESLLPVKDSLEAAIAIPAATPEQLLEG
                     VHATLRQLSTALERNRVVEISPAAGAKFDPHQHQAISVVPADQEANTVVAVLQKGYLI
                     SDRVLRPALVTVTAPK"
     gene            complement(3016..3408)
                     /locus_tag="PKV56_16900"
     CDS             complement(3016..3408)
                     /locus_tag="PKV56_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOX69539.1"
                     /translation="MRLDKWLWAARFYKTRSLATDEIGKGRISVNAQAAKPSRELRPG
                     DRIELRQGAVLRTVVVVALSDSRGPAPAAQALYAETEESIAAREKAARERREAAEPSL
                     SIEQGRPTKRDRRKLADWNRWSASVDSE"
     gene            complement(3461..4558)
                     /gene="hemH"
                     /locus_tag="PKV56_16905"
     CDS             complement(3461..4558)
                     /gene="hemH"
                     /locus_tag="PKV56_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOX69540.1"
                     /translation="MPFAPEPDHEHGKPPSPDTAVLLCNLGTPDAPTAQALRRYLKEF
                     LSDPRVVEIPRLLWWFILNVVILRVRPKQSAAKYATVWTDQGSPLKVWTEKQAAMLQG
                     YLGERGHRVLVRPAMRYGAPAIASVLDELRSAGATRVLVLPLYPQYSGPTTASVCDAV
                     FEWGKRARRLPEFRFVNQYHDDPAYIGALAKRVSDHWKHSGRAERLVLSFHGVPRRTL
                     MLGDPYHCQCLKTARLLGERLGLGKHELVVTFQSRFGKAEWLQPYTEPTLVALAGQGI
                     KRVDIMCPGFTADCLETLEEIAQEARTAFLNAGGTEFHYLPCLNDQHEWLVALTGIAI
                     NHLQGWNCTVVADATAQEMQRERALALGADR"
     gene            complement(4608..5552)
                     /locus_tag="PKV56_16910"
     CDS             complement(4608..5552)
                     /locus_tag="PKV56_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX69541.1"
                     /translation="MTRNLERCVLFADLRGSTGLYETLGNAVATQLVTQSIAMLGRVV
                     GACGGTLVKTLGDGLMATFIDATSAVKASIDMHEALSRGGLDEAVGAAGRALRLQVGL
                     AYGEVVEMAGDYFGDAVNVAARLLEHSGDNETLATSTVLDELAAFDRVQFRRLDHLQL
                     RGRVEPVEVHVLETHHFTDMAATAFGDIIMAPEPEGVRLTWLDVNQVFATDSMPVVLG
                     RSTQATYCIDDNRVSRSHARIDWHGGAFQLSDLSYNGTYVRFTGDTEVVALRRGTCTL
                     HGSGVIGLGTAPSDPTAPCVRFEVLRFSDTLRQPPAEI"
     gene            complement(5682..>5926)
                     /locus_tag="PKV56_16915"
     CDS             complement(5682..>5926)
                     /locus_tag="PKV56_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX69542.1"
                     /translation="AMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMSTDHTLEEVGK
                     QFDVTRERIRQIEAKAIRKLKHPSRSDKLRTYLDNL"
BASE COUNT          876 a         1870 c         1922 g         1258 t
ORIGIN      
        1 cagcacgctt ctgcgtgtcc gagagcatct cgtaggcttc cttggcctcc ttgaatttct
       61 cctcggcctt cttcgcatcg tcgccctgat tgcggtcagg gtggtgcttc atcgccagct
      121 tgcgataggc cttcttgatg tcttcctcgg acgcgttctt ggccacgccc agggtttcgt
      181 agtaatcacg ctttgccatc ggttcatccg tcacgccgca gcgtctggtt caaatgagaa
      241 cgccgcgtcg ggcctcggcc tgcgctcgag caccctgacg cggcgaggac gccgggcgaa
      301 cccggcgtct ttggcctatc gaagtcgtgt cacttcttgt ctttgacttc ggtgaattcg
      361 gcatcgacca cgttgtcgtc agcaggcttg tccgagggcg ccgccgaagc acccgctcca
      421 ccagcagatg cggcttcggc tgcaccggca gcggcctgtg catcggcgta gaccttctcg
      481 ccgagcttct gactggcggt catcagggcc tctgtcttgg cctcgatggt cgcctgatcc
      541 tcgctcgtca gcgactcttc ggcgtccttc aatgcggcct cgatcttttc cttttcaccc
      601 gcttcaagct tgtcgccatg ctcgcccagg ctcttcttga cgctgtggat catcgcgtcg
      661 gccgaattgc gagcctggat caggcccagc ttcttcttgt cctcggctgc attcagctcg
      721 gcatccttca ccatctgctg gatctcggct tcggtcagac ccgagttggc cttgatggtg
      781 atcttgtttt ccttgcccgt gcccttgtcc ttcgcaccga cgtgcaggat gccgttggcg
      841 tcgatgtcga agctcacctc gatctgcggc gtgccgcgtg cggcaggcgc gatgccctcg
      901 aggttgaact cgcccaggct cttgttgccc gaggccatct cgcgctcacc ctggtagacc
      961 ttgatcgtca ccgcgggttg attgtcatcg gcggtggaga aggtctgcgc gaacttggtc
     1021 gggatggtcg tgttcttctt gatcatcttg gtcatcacgc cgcccagggt ctcgatgccc
     1081 aggctcagcg gggtgacgtc gagcagcagc acgtccttgc ggtcgccggc cagcacctgg
     1141 ccctggatcg ccgcgcccac agccacggct tcatccgggt tgacgtcctt gcgcggctcc
     1201 ttgccgaaga actccttgac cttgtcctgc accttgggca tgcgcgtcat gccgccgacc
     1261 aggatcacgt cgtcgatggc gctcaccgcc acgcccgcat ccttgatggc ggtgcggcac
     1321 ggcgcgatgg tgcgctcgat cagctcttcg accagcgatt ccagtttggc gcgcgtgagc
     1381 ttgatgttca ggtgcttcgg gcccgaggcg tcggccgtca cgtagggcag gttgatgtcg
     1441 gtctgcgtcg cgttcgacag ctcgatcttg gccttctctg ccgcttcctt caggcgctgc
     1501 agcgcgagca cgtccttcga caggtcgacg ccttgctcct tcttgaactc ggagacgatg
     1561 taatcgatga tgcgctggtc gaagtcctcg ccgccgagga aggtgtcgcc gttggtcgac
     1621 agcacttcga actgcttctc gccgtcgacg tcggcgatct cgatgatcga gatgtcgaag
     1681 gtgccgccgc ccaggtcata gacggcgatc ttgcggtcgc ccttgccgtg cttgtccagg
     1741 ccgaaggcca gtgccgcggc ggtcggctcg ttgatgatgc gcttgacgtc caggcccgcg
     1801 atgcggccgg cgtccttggt ggcctggcgc tggctgtcgt tgaagtacgc aggcaccgtg
     1861 atcaccgctt cggtgacctc ttcaccgaga tagtcctcgg cggtcttctt catcttgcgc
     1921 agcacttcgg cgctgatctg cggcggcgcc agcttcttgc cacgcacctc gatccaggcg
     1981 tcgccgttgt cggcggccgt gatcttgtag ggcatcaggt cgatgtcctt ctgcacttcc
     2041 ttctcggtga acttgcggcc gatcaggcgc ttcactgcgt acagcgtgtt cttcggattg
     2101 gtcactgcct ggcgcttggc cgaggcaccg acgaggatct cgccgctttc ctggtaggca
     2161 acgatcgatg gcgtggtgcg agcaccctcg ctgttctcga tcaccttggt ggtgttgcct
     2221 tccatgatcg acacgcacga gttggtggtg ccgaggtcga taccgatgat ttttgccatg
     2281 tcgaattctc ctgagagctg aaactgtcct gatgacgagt tgtggataac tttcgtcttt
     2341 tcaagggtgg ctgccggccg gaatcggcac ggagcctctg tgcgcaaccg ctactttggt
     2401 gcggtgacgg tgaccagtgc agggcgaagc acgcggtcgg aaatgaggta gcccttctgc
     2461 agcacggcga ccacggtgtt ggcctcctga tctgccggca cgacgctgat ggcctggtgc
     2521 tgatgggggt cgaacttggc gccagccgcg ggactgatct cgacgacgcg attgcgttcg
     2581 agcgcagtcg acaactggcg cagggtggcg tgcacgcctt caagcaattg ctcgggagtc
     2641 gcggccggga tggcgatggc ggcctcgagg ctgtccttga ccgggagcag gctctccgca
     2701 aagccttcta cggcgaactt gcgggccttc gagatttctt cctcggcgcg tcggcggatg
     2761 ttctcggctt cggccttggc tcgcaggtag gcgtcggata cctcggcgtg cttggcttcc
     2821 agctcggcga gcttcgataa cgggtcgctg gctgcgctgc ccggggccgc cgtgccggcg
     2881 gcgtcgctgg gggatggtgt ctgagattct gtttggctca tgtcggctga attcccttca
     2941 agcccccaaa tggggctgat cccccagctt tcaagaggga ctttgcaggg ccttggcagt
     3001 gaccgaacgg cagcctcatt cgctgtcgac agaggcgctc cagcgattcc agtcagccag
     3061 cttgcggcgg tcgcgcttgg tgggtcgacc ttgctcgatc gacagcgacg gttccgcggc
     3121 ttcgcggcgt tcgcgcgctg ccttctctcg cgccgcgatg ctttcctctg tttcggcata
     3181 gagcgcctgg gcggccggag ccggtccgcg gctgtcgctc agggcaacca cgacgaccgt
     3241 gcgcagcacc gcaccttgac gcaactcgat tcggtcgccc gggcgcagct ctcgcgatgg
     3301 cttggcagcc tgcgcattca cgctgatgcg ccccttgccg atttcatcgg tcgccaggct
     3361 tcgtgtcttg tagaagcggg cggcccagag ccacttgtcc agtctcaaag gcttgccctg
     3421 cgtgtcgggt ggtcaggtgc gcggcgacac gcctgattcg tcagcgatcc gcgcccagcg
     3481 ccaaggcgcg ttcgcgttgc atctcttgcg cggtcgcatc cgcgacgacg gtgcagttcc
     3541 agccctgcag gtggttgatc gcgatgcccg tgagcgcgac cagccattcg tgctggtcgt
     3601 tcaggcaggg caggtagtgg aactcggtgc ccccggcgtt gaggaaggcg gtgcgtgctt
     3661 cctgtgcgat ttcttccagg gtttcgaggc agtcggcggt gaatcccggg cacatgatgt
     3721 cgacacgctt gataccttga ccagcgagcg cgaccaaagt gggttcggta tagggctgca
     3781 gccattcggc cttgccaaag cggctctgga atgtcaccac cagctcatgc tttccgaggc
     3841 cgagccgctc accgagcagg cgggcggtct tcaggcactg gcagtggtag gggtcgccca
     3901 gcatcagcgt gcggcgcggc acaccgtgga agctcagcac cagcctctcc gcccggccgg
     3961 aatgtttcca gtgatcggag acgcgtttgg ccagtgcccc gatataggcc gggtcatcgt
     4021 ggtactggtt gacgaatcgg aactcgggca gtcggcgcgc ccgcttgccc cactcgaaga
     4081 cggcgtcgca tacgcttgcc gtggtgggcc ccgaatactg tggataaagc ggcagcacga
     4141 gcacgcgtgt ggcccctgct gaacgaagtt cgtcgagcac gctggcgatg gccggggctc
     4201 cgtaacgcat ggcggggcgc accagcacgc gatggcctcg ctcgccgagg tagccctgca
     4261 acatggcggc ctgcttctcg gtccagactt tgaggggtga gccctgatcg gtccagacgg
     4321 tcgcgtactt ggcagcagac tgcttgggcc tcacacgcag gatcacgaca ttcaggatga
     4381 accaccacag caaccgtgga atttcgacga ctcgggggtc gctgaggaac tccttcaggt
     4441 agcgccgcag cgcctgcgca gtgggtgcgt ctggagtgcc caggttgcac aggagaactg
     4501 ccgtgtcggg cgaagggggc ttgccatgtt cgtgatcagg ctcgggagcg aatggcatgc
     4561 ggcgtccggt gggctaaccc gtgattatcg gtgagtgccg cctcgcttca gatttcggct
     4621 ggcggttgcc gcaaggtgtc ggaaaaacgc agcacttcga agcgaacgca gggcgccgtt
     4681 gggtccgagg gcgccgtgcc gagaccgatc acaccgctgc cgtgcaaggt gcacgtgccg
     4741 cgccgcaggg cgacgacctc ggtgtcgccg gtaaatcgca cgtaggtgcc gttgtagctg
     4801 aggtctgata gctggaacgc gccaccgtgc caatcgatgc gcgcgtggga gcgggagacg
     4861 cggttgtcgt cgatgcagta cgtcgcctgc gtgctgcgtc cgagcacgac aggcatgctg
     4921 tcggtggcga agacctggtt cacgtcgagc caggtgagcc gcacgccttc gggctctggc
     4981 gccatgatga tgtcgccgaa cgcggtagcc gccatgtccg tgaagtggtg cgtctcgagc
     5041 acatgcactt cgaccggctc gacgcggccc cgcaattgaa ggtgatcgag gcggcgaaac
     5101 tggacccggt cgaacgctgc caattcgtcc agtaccgtcg aggtggccag cgtctcgttg
     5161 tctccggagt gctcgagcag tcgcgccgcg acgttgaccg catcgccgaa gtagtcgccg
     5221 gccatctcga cgacttcccc ataagccaga ccaacctgca gccgcagagc ccggccggct
     5281 gccccgaccg cctcgtccag cccgccgcgc gacaacgcct cgtgcatgtc gatcgaggcc
     5341 ttgacggctg aagtggcgtc gatgaaggtc gccatcagcc catcgcccag tgtcttgacc
     5401 agcgtaccgc cgcaggctcc taccacgcgc ccgagcatcg cgatgctttg ggtcaccaac
     5461 tgcgtggcga cggcgttgcc gagcgtttcg tagaggccgg tacttccgcg caggtcggcg
     5521 aacagcacgc aacgttcgag gtttcgagtc atgccttggt gactgtgaat tgcgtcacag
     5581 tctatcgcca accagccatg aaaaaggccc cacacaaggg tggggcccga ttgaactgcg
     5641 agaaggctca gggtgcgccg gactgtctgt cacctgcgcg gtcacaggtt gtccaggtag
     5701 gtccgtagct tgtccgagcg gctggggtgc ttgagcttgc ggatcgcctt cgcctcgatc
     5761 tggcgaatcc gctcgcgggt cacgtcgaac tgcttgccga cttcttccag cgtgtggtcc
     5821 gtgctcatct cgatgccgaa gcgcatgcgc agcaccttgg cctcgcgtgg agtcaggctg
     5881 tcgaggatgt ccttcaccac gtcgcgcagg ccggcctgca tggcgg
//
LOCUS       DAOORV010000200         5912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16348_length_5912_cov_5.364350, whole genome shotgun sequence.
ACCESSION   DAOORV010000200 DAOORV010000000
VERSION     DAOORV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16348_length_5912_cov_5.364350"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /gene="lon"
                     /locus_tag="PKV56_16920"
     CDS             <1..1626
                     /gene="lon"
                     /locus_tag="PKV56_16920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOX69543.1"
                     /translation="EARKKADAEYKKLKLMSPMSAEATVVRNYIDTLVNLPWTKKTKI
                     KHDLAFAEAVLNEDHYGLEKVKDRILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQ
                     SVARATGRKFVRMALGGVRDEAEIRGHRRTYIGSMPGKVLQSLSKVGTRNPLFLLDEI
                     DKLGMDFRGDPSSALLEVLDPEQNHTFSDHYVEVDFDLSDTMFIATSNSMNIPPALLD
                     RMEVIRLSGYTEDEKVNIAQRYLLPKQAKNNGVKDAEMEVAESAVRGIIRYYTREAGV
                     RSLEREISKICRKVVKSIQLKQVTEKIVVTADNLNDFLGVRKYDYGQAEKQNQVGQVV
                     GLAWTEVGGDLLTIESAMMPGKGVIIRTGSLGDVMKESVEAARSVVRSRARRLGIKDE
                     LFEKRDIHIHVPDGATPKDGPSAGAAMTTAFVSALTGIPVRADVAMTGEITLRGEVTG
                     IGGLKEKLLAAHRGGIKTVIIPEENVKDLQDIPENVKNQLEIVPVKWIDQVLEVALQS
                     LPQALPDEEPPKPEEVKPEAAAVPAVSTESMKH"
     gene            1697..1772
                     /locus_tag="PKV56_16925"
     tRNA            1697..1772
                     /locus_tag="PKV56_16925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1730..1732,aa:Gly,seq:gcc)
     gene            1833..1906
                     /locus_tag="PKV56_16930"
     tRNA            1833..1906
                     /locus_tag="PKV56_16930"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1865..1867,aa:Cys,seq:gca)
     gene            2348..4036
                     /locus_tag="PKV56_16935"
     CDS             2348..4036
                     /locus_tag="PKV56_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOX69544.1"
                     /translation="MAHSISTALTSRVATWLRLMFGLLILFTLAQAAGAEPAAASAPE
                     TADIAPLVIGHRPIHTFRVPLGAFTASERADGARMRILKAFEESGEGWTSVRPQGGGV
                     QVELDGKPLFVVLPGDAAQLTETSVEQMANSASHALQKAWAEARERRDPRATLGAVLR
                     VVLALVALAVVLLAVWRAARWVRDVVTSALITRAQFLADATLATRITPIFLGTISRSC
                     VLLGWLLSLLALFMCLAYSLDQFAITRALGEGLFHAFFDLLLESLASVASALPGLFFA
                     VLIFLAAWVGTQVSTEVFNHVASGRLKLGMLDSHTAPATRRIVNACMWLFALAMAYPY
                     LPGAQSEAFKGLSVILGIMVSIGASGLVGQIASGVILVYTRALSLGEYVSVQDCEGTV
                     TEIGLFVTRLRTGLGSEYALPNTLVLSNVTRNFSREAQGPGHVLETAVTIGYDTPWRQ
                     VHALLLGAAESVVDIARQPGPYVVQSGLSDFYVVYKLVVYVAVGSPSQRAQAASALNA
                     SIQDMFNRYGVQIMSPGYYEDPAHPKVVPEDQWYAAPARRPDEAQVATDARNRN"
     gene            complement(4018..4101)
                     /locus_tag="PKV56_16940"
     tRNA            complement(4018..4101)
                     /locus_tag="PKV56_16940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4065..4067),aa:Leu,seq:taa)
     gene            4405..5154
                     /locus_tag="PKV56_16945"
     CDS             4405..5154
                     /locus_tag="PKV56_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX69545.1"
                     /translation="MKVLVAVKRVVDYNVKVRVKSDGSGVDLANVKMSMNPFDEIAIE
                     EAVRLREKGVVTEIIAVSCGVLACQETLRTAMAIGADRAILVETDVELQPLAVAKLLK
                     SLVEREQPGLVILGKQAIDDDCNQTGQMLAALGDMPQATFASKVEVSDGCARVTREVD
                     GGLETLKLTLPAVVTTDLRLNEPRYVTLPNIMKAKKKPLEVLKPADLGVDVTPRIKTL
                     KVSEPPRRAAGIKVPDVATLISKLKTEAKVI"
     gene            5156..>5912
                     /locus_tag="PKV56_16950"
     CDS             5156..>5912
                     /locus_tag="PKV56_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX69546.1"
                     /translation="MATLVIAEHDNASLKAATLNTVAAALKIGGAVDVLVAGQDAAAA
                     AQAATAIPGVGKVLHADAPCFAHGLAEAVAAQVLALVPATGSAYTHVLFAATASGKNI
                     APRVAARLDVAQISDITKVVSADTFERPIYAGNAIATVQSADAVKVITVRSTGFDPVA
                     GEGGSAAVDVIAPVADAGKSSFVGSEIARNDRPELTAAKIIVSGGRALGSVEKFNEVL
                     TPLADKLGAALGASRAAVDAGYAPNDWQVGQTGK"
BASE COUNT         1094 a         1786 c         1909 g         1123 t
ORIGIN      
        1 gaggctcgca agaaggccga tgccgagtac aagaagctca agctgatgtc gccgatgtcg
       61 gccgaggcga cggtggtgcg caactacatc gacacgctgg tcaatctacc gtggaccaag
      121 aagaccaaga tcaagcacga cctggccttc gctgaagccg tcctgaacga agaccactac
      181 ggcctcgaga aggtcaagga tcgcatcctt gaatatctcg cggtgcagca gcgtgtcgac
      241 aaggtgaagg caccgatcct gtgtctggtc gggcccccgg gtgtcggcaa gacctcgctc
      301 gggcaaagcg tcgctcgcgc caccggccgc aagttcgttc gcatggccct tggtggcgtg
      361 cgtgacgagg ctgagattcg cgggcatcgt cgcacctaca tcggctccat gccgggcaag
      421 gtgctgcaga gcctgagcaa ggtcggcacg cgcaatccgc tcttcctgct cgacgagatc
      481 gacaagctgg gcatggattt ccgcggcgat ccctcgagcg cgttgctcga ggtgctcgac
      541 ccggagcaaa accacacctt cagcgatcac tacgtcgagg tcgacttcga cctttcggac
      601 acgatgttca tagcgacttc caattcgatg aacatccctc cggcgttgct ggatcgaatg
      661 gaagtgatac gcctctcggg ttacaccgag gacgagaagg tcaacatcgc tcaacgctat
      721 ctgttgccta agcaggccaa gaacaacggc gtcaaggatg ccgagatgga agtcgccgag
      781 tcggccgtgc gggggatcat ccgctactac acccgtgagg ccggagtgcg ttcgctcgaa
      841 cgtgaaatct cgaagatctg ccgcaaggtg gtcaagagca ttcagctcaa gcaggtgacc
      901 gagaagatcg tggtcacagc cgacaacctg aatgatttcc tgggggtgcg caagtacgac
      961 tacggccagg ccgagaagca gaatcaggtg ggtcaggtcg tcggtttggc gtggaccgag
     1021 gtcggcggag acttgctcac catcgagtcg gcaatgatgc ctggcaaggg cgtgatcatt
     1081 cgtaccggct ccctcggcga tgtgatgaag gaatcggtcg aagcagcgcg atccgtggtg
     1141 cgctcacgtg ctcgccgcct ggggatcaag gacgagctgt tcgagaagcg tgacatccac
     1201 attcacgtgc ccgatggcgc gaccccgaag gatgggccca gcgccggcgc ggccatgacc
     1261 acggccttcg tttctgcgct cacgggcatt cctgtccgcg cggacgtcgc catgaccggg
     1321 gagatcacac tgcgcggtga agtcaccgga atcggtggac tgaaggaaaa gctgctggcg
     1381 gcccaccgtg gcggcatcaa gacggtgatc attccggaag agaacgtcaa ggaccttcag
     1441 gacattccgg agaacgtgaa gaaccagctc gagatcgttc cggtgaaatg gatcgaccag
     1501 gtgctcgagg ttgctttgca aagcctgcca caagcgctgc cggatgaaga gccgcccaag
     1561 cccgaagagg tgaagcctga agctgcagcc gttcctgccg tgtcgaccga atcaatgaaa
     1621 cactgattca tttaatggag tcgatgcaga ggtttcgatg tatactgcga gacgcgatgt
     1681 gcagtgcatc gcgaatgcgg gagtagctca gttggtagag cgcaaccttg ccaaggttga
     1741 ggtcgcgagt tcgagcctcg tctcccgctc caaaaaatag aaaaagggaa gcaccagctt
     1801 ccctttttcg tacctgggtc ggcgacccgt acggcgcggt agcaaagcgg ttatgcaccg
     1861 gattgcaaat ccgattagcc cagttcgact ctgggccgcg cctccatcct ccagtgcacc
     1921 tcatgcagcc gtcgcccgat gcgtccggtc ggaggtcggg cgaggtcttt tccctctcct
     1981 gcttcgccat gaacgcatgg atcgccagcg tgtgcccgct ccgctcacat ctactcctgg
     2041 cagtcgacgg gattgcgctg gcagttcctg tgcactggcc ggcgaatagc aacggccggc
     2101 tgcgcggata catttagttc aacgcgtggc aaggcaggac aactcccctg ttcgcacgga
     2161 ctgactctgc accggtcact tcgttgccca ttcatcgacc ggacgggtcg caagcggtca
     2221 gggttcggtt gagcagggca gggagccggg tcgcgcaagt tcgcttgcaa ctacagcgtg
     2281 gtagtgccca ttttttccgt gcgagaagaa gatgaatgca tgctcctgtc tgcggcgtga
     2341 ggttgccttg gctcactcca tttccacagc cctgacttcg cgcgtcgcga cctggctgcg
     2401 actgatgttc gggctgctga tcctgttcac actggcacag gctgccggtg cggaacccgc
     2461 agcggcatcg gcacccgaaa ctgctgacat cgcgcctctg gtgattggcc atcgtccgat
     2521 ccataccttt cgcgttcctc tgggagcgtt cacggccagt gagcgggctg acggggcccg
     2581 gatgcgcatc ctgaaagcct ttgaggagtc aggggaaggc tggacctctg ttcgtccgca
     2641 ggggggcggt gttcaggtcg aactcgatgg caaacctttg ttcgtggtgc ttccgggaga
     2701 tgctgcgcag ttgaccgaaa ccagcgtcga gcagatggcc aactccgctt cacatgcctt
     2761 gcagaaggcc tgggccgagg cccgcgagcg ccgtgacccg cgagccaccc tcggcgccgt
     2821 gctccgtgtc gtgctggcgt tggtggcgct ggctgtcgtg ctgctggctg tctggcgcgc
     2881 ggcgcgctgg gttcgtgacg tcgtcaccag cgccctgatc acgcgcgcgc agttcctggc
     2941 tgacgccacg ttggccacgc gcatcacgcc gatctttctc ggaaccatat cgcggtcgtg
     3001 cgtgctgctg ggatggttgc tcagccttct ggcgctcttc atgtgtctgg cctacagcct
     3061 ggaccagttc gcgatcacga gggcgcttgg cgaaggtctt ttccatgcct tcttcgacct
     3121 gctgctggag agtctggcct cggtggcatc ggccctgcct ggcttgttct ttgccgtgct
     3181 gatcttcctg gctgcctggg tcggaacgca ggtatcgacc gaagtcttca accacgttgc
     3241 ttcgggtcgt ctgaaattgg gcatgctgga cagccacacc gccccggcga cgcgacgcat
     3301 cgtcaacgcc tgcatgtggc tgtttgcgct ggccatggcg tatccgtatc tgccaggcgc
     3361 tcagagcgag gctttcaagg gcctgtcggt gatcctcggg atcatggtct ccatcggggc
     3421 ttcgggcctg gtgggccaga ttgccagcgg cgtgatcctt gtctataccc gggcgctgtc
     3481 gctgggcgag tacgtgtccg tccaggactg cgagggcacc gtgacagaga tcggactgtt
     3541 cgtgacgcgc ctgcgcacgg gcctgggatc ggagtacgcg ttgccaaaca ccctggtact
     3601 cagcaacgtg acgcgcaatt tctcgcgtga ggcgcaggga cccggccatg tgctggagac
     3661 cgcggtcacc atcgggtatg acacgccgtg gcgtcaggtg catgccctcc tgctgggcgc
     3721 tgccgagtcc gtggtcgaca ttgcgcggca gcctggtccc tacgtagtcc agagcgggtt
     3781 gtcggatttc tatgtcgtct acaagctggt ggtttacgtg gccgttggca gtccgtcgca
     3841 gcgcgcccag gcggcgagtg cgctcaacgc cagcatccag gacatgttca accgctatgg
     3901 tgtgcagatc atgtcgccgg gctactacga ggaccccgcg caccccaagg tggtgcccga
     3961 ggatcagtgg tatgccgcac cggcgcgacg cccggacgaa gctcaggtcg ccactgatgc
     4021 ccggaaccgg aattgaaccg gtacggcctc tcggcctgcg gcttttaagg ccgctgcgtc
     4081 taccaatttc gccatccggg cacgccgttg cggatgatag ggggcactca acgtccgcaa
     4141 gtgccaaccg cgaaccgctg caagcgaact gcaatgcagt ccgggccgcg tccgcccgcc
     4201 agagagctgg tcggacatgg ggcttacgat tccgatttcc ggcgcttggc cttgacttga
     4261 agcctgctgc aaggcgtccg gccgcgtcat gattcagctc tgttccagga accatccaga
     4321 cgttcatggt ggcactccgc gtgcacggga ggtcgcctta aaatcgaatg tttggtggtc
     4381 accccgaccg caggagccga gtgaatgaag gtgctggtgg cagtcaagcg tgtcgttgac
     4441 tacaacgtca aggtgcgcgt caagtcggat ggcagcggcg tcgatctcgc caacgtcaag
     4501 atgagcatga atccgttcga cgagatcgcc atcgaagagg ccgttcgatt gcgcgagaag
     4561 ggcgtggtca ccgagatcat cgccgtgtcg tgcggagtgc tggcttgcca ggaaactctg
     4621 cgtaccgcga tggccatcgg ggccgatcgc gcgatcctgg tcgagaccga tgtcgagctc
     4681 caacctctgg ccgtcgccaa actcctgaag tcgctggtcg agcgtgagca gcccggactt
     4741 gtcatcctcg gcaagcaggc catcgacgat gactgcaacc agacgggaca gatgcttgct
     4801 gccctgggcg acatgccgca ggccaccttt gcctcgaagg tcgaggtgtc tgatgggtgt
     4861 gctcgggtca cccgcgaggt cgatggtggc ctggaaacac tgaagctcac tctgccagcg
     4921 gtcgtcacga ccgaccttcg gctcaacgaa ccgcgctatg tcacgttgcc gaacatcatg
     4981 aaggcgaaga agaagccact cgaggtcttg aagcccgccg atctcggggt cgatgtgact
     5041 ccacgcatca agaccttgaa ggtcagcgag ccgcccaggc gtgccgcggg catcaaggtg
     5101 ccggacgtgg ccacgctgat ttcaaaactc aagaccgaag cgaaggtgat ctgacatggc
     5161 caccctggtg atagccgaac atgacaacgc gtcgctgaag gccgcgaccc tcaacaccgt
     5221 cgccgcggcg ctgaagatcg gaggtgcagt cgatgtgctc gtggctgggc aggatgccgc
     5281 ggcggctgcg caggctgcga cagcgattcc cggtgtcggc aaggtgctgc acgcggatgc
     5341 tccctgtttc gcgcacggtc tggccgaagc cgtcgcggcc caggtgctgg cgctggttcc
     5401 ggccaccggg agcgcctaca cgcacgtcct ctttgccgcc acggcgtcgg gcaagaacat
     5461 tgcgccgcgc gtggctgcga ggctcgacgt ggcacagatc tccgacatca cgaaagtggt
     5521 gagcgcggac accttcgagc gtcctatcta cgcaggcaat gcgatcgcca ctgtgcagag
     5581 tgcagacgcc gtcaaggtca tcacggtgcg atccacgggc ttcgatccgg ttgcgggtga
     5641 gggtggctcc gcggcggtcg acgtgattgc gccggtggca gatgccggca aatccagttt
     5701 cgtcggtagc gagatcgccc gcaacgaccg gccggagctg accgcagcca agatcatcgt
     5761 cagcggcggc cgtgccctgg gcagcgtcga gaaattcaat gaagtgctga cgcccctggc
     5821 cgacaagctc ggcgcggccc tgggtgccag ccgcgcagca gtcgacgcag gttatgcgcc
     5881 gaacgactgg caggtcgggc agaccggcaa ga
//
LOCUS       DAOORV010000201         5850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16560_length_5850_cov_12.414323, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000201 DAOORV010000000
VERSION     DAOORV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16560_length_5850_cov_12.414323"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKV56_16955"
     CDS             complement(<1..830)
                     /locus_tag="PKV56_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69547.1"
                     /translation="MIVKLWSIAAPTSPDATHSPIPTNVVRGLVALWTGSTQTSADTQ
                     AAAAFLIDLHDQGCHIACGCRGEDPHRAPRLLCKKIRLTRTVFLCRDTTRPAHAESCI
                     FRRDSRSDVDHEAAQRAERTARQYDLADLLSGTGGVIVQRARSPEWARPRALQDSHGQ
                     LTPPTIATALDLLLEASGANAAHVPYPHTPEGHRIFPEQSILEATSRVSHQDTAAYTL
                     DSLFLVQPSSLEGFERVTYEHAAAGWRGDGAPVGYAMFVCDDILQAGSGSLLRYRGGE
                     "
     gene            1902..2894
                     /locus_tag="PKV56_16960"
     CDS             1902..2894
                     /locus_tag="PKV56_16960"
                     /inference="COORDINATES: protein motif:HMM:NF024244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HOX69548.1"
                     /translation="MKTFLKLVGEFLPRYRKNFRKMGLADQVQTVVRDLGGHHQTKNK
                     DTGWRTKRDRACIVHAAIRRLKIKNVLSISQKHIGQILHAWAREGIAAATFQTRLSHL
                     RWFTSAIGKPGMVRSVSYYGLSDDLVRRTYVATTTKSWAGNGIDPQSMIEEIAKEHEY
                     VACQLEGQQLFGLRTSESMQICPTEDRQGDMLVVSRGTKGGRTRVIPIETQAQYNWLR
                     KAAELAALTNRGNLIEGDRSYKQARNHRNYVARKFGITKTELGVVPHGLRVQFAEERY
                     ELVTGAPAPTRGGGCVTGEEDRQGRVEVSRVLGHSRESIAGAYIGARPQNQHIG"
     gene            2981..3923
                     /locus_tag="PKV56_16965"
                     /pseudo
     CDS             2981..3923
                     /locus_tag="PKV56_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043745823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(4205..4789)
                     /locus_tag="PKV56_16970"
     CDS             complement(4205..4789)
                     /locus_tag="PKV56_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69549.1"
                     /translation="MPPITCEACRQPAADHDIVNYGSMDAGYQRLCGRCYNEAAAGRL
                     GLQGFEHVAFEPIRMLDGRGAEHEFRFRNWLCGTGLAIDAFELRDGGLAGYRFQVIGE
                     PHDDPLELLGKLIGKMRRALALTHLEDTDHGPQVNDGLVLRGSVDSDPDADHRVPMVV
                     IDGREISWEQFGRMVATFEGWQFRLEFRDRSEEV"
     gene            complement(4826..5017)
                     /locus_tag="PKV56_16975"
                     /pseudo
     CDS             complement(4826..5017)
                     /locus_tag="PKV56_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5390..>5850)
                     /locus_tag="PKV56_16980"
     CDS             complement(5390..>5850)
                     /locus_tag="PKV56_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69550.1"
                     /translation="DYVKFIRMWLNDGRSDSGEQILRPETVEMASRNHLPKGMHIKML
                     PGVGATTTAASILPVSKPRLSNDAEFFPGMPKTWALTFMINEEEAPTGRPAGALAWAG
                     LANLYYWIDRANGVGGYWATQIFPFADPTSVVGFLAMEKATYQHAKLRAR"
BASE COUNT         1067 a         1878 c         1860 g         1045 t
ORIGIN      
        1 tgctcgcctc ctcgatacct gagcagcgac ccgctgcccg cctgcaggat gtcgtcgcac
       61 acgaacatgg catacccgac cggcgcgccg tctccgcgcc aacctgccgc cgcatgctca
      121 tacgtgacac gctcaaagcc ctccaaactt gagggctgga ccaagaagag cgagtcgagt
      181 gtgtacgccg cagtatcttg gtgtgagact cggctcgtcg cttcgaggat tgattgctca
      241 ggaaagatcc ggtgtccttc tggcgtgtga gggtacggca catgcgctgc attggcgcct
      301 gacgcctcaa gaagcaaatc cagcgcggtt gcaatggtcg ggggcgtcaa ctgaccatgt
      361 gaatcctgaa gggctctcgg tctagcccat tcaggggagc gggctcgctg cacgatcacg
      421 cccccagttc cggataaaag atctgcaaga tcgtattggc gtgcagtacg ttcggcgcgc
      481 tgcgcggcct catggtccac gtcgcttcta gaatccctgc gaaagataca ggattccgca
      541 tgcgccgggc gcgtcgtatc tctgcacaag aaaaccgtcc tggtcaagcg aattttcttg
      601 cacagcaagc gcggcgcgcg gtgcgggtcc tcaccgcgac aaccacacgc aatgtggcaa
      661 ccttgatcgt gaagatcgat cagaaacgcc gccgcagcct gcgtatcggc gctggtttgc
      721 gtgctgccgg tccacagggc cacgagtcca cgcaccacat tcgttggaat cggtgaatgc
      781 gtagcgtctg gcgacgtggg cgcggcgatg ctccagagtt tcacgatcat caattcgagt
      841 cggggcgagc agcataccgc tgctgctggc gctgccgagt tggcaactgc gttggctgca
      901 agtgcttgaa ccgcgcgctg gtcacccgcc agatctccgc gcagccgtcg cgggacgaca
      961 tgccggagga tcgcgcgtcg ttcagcacat agagaggcga cggcgcccac ctatggggga
     1021 ctatgcccag tcagcagaat cttgtcggga gcaaatgttg acttctgctg gccagttgac
     1081 acggcgacgc cggcggatta ccgtcaggtc atcgttgatg ggctgaggcc caataggcgc
     1141 tgtacagcgc cgcgatctac gggttggcga atgcgatctc gcatgatgct gagctgttaa
     1201 atttgttgtg cctctgcgcg cacaaccaca ccatcgctgc agtcggcctt gtgctctcag
     1261 cgaaaaccaa gtgcatagcc cgcattccgc aggcttgcgc tacacaccct ccaggctttg
     1321 gcgcctggtg gagggtccgt gtccctttgt ggacgcgaca aaccctgcca tcagttcgct
     1381 agcctgaacc atgccccata gctgacgtgc agcgtgatct cgctgggacg gatggtgcgg
     1441 tacgcccttt gtggctcctg ccgcgccgga actcccggtc gtgtgatccg cgatggcgtc
     1501 tgggcttgcg tccgcggcgg atgaccccgc cccccgcaag cagggcacag agcgccgcat
     1561 cgctggtggc aggcgatgtc agggcggcgc tcgcccatct gggattcccc ggagtatccg
     1621 gacccgagcg gccagcctga gaaatcagag gccgccgacc caggccaacc cctcaagacc
     1681 cccgcctcac ccctgatttt taactggccc ggcgcctgcg acagcaggcg ctggatcctc
     1741 ttccattcac gcaggagatc catctcaaat ttgcgtcacc cgacgtagca aacagcgtgc
     1801 ggaccaatcc gcacccgttg cagtcatggg ttggcgcgac cgtgcctgaa agccgcctca
     1861 ccagcgattc aaggcgaatt caagaatcaa ggagcatcca tatgaaaacc tttctcaaac
     1921 tcgttgggga atttcttcct cgatatcgga agaatttccg gaaaatgggc ctggcagacc
     1981 aggttcagac ggtagtccgc gatctcggtg gccatcatca aaccaaaaat aaggacaccg
     2041 gctggcggac caagcgggat cgcgcgtgca tcgtgcacgc ggccatccgg cggctgaaaa
     2101 ttaagaacgt gctctccatc agccagaagc acatcggcca gattctgcac gcgtgggcgc
     2161 gcgaggggat cgctgctgcg acgttccaga cccggttgtc ccacctgcgc tggttcacca
     2221 gcgccatcgg caagccgggc atggtccgtt cggtcagcta ttatgggttg tccgacgacc
     2281 tggtccgccg tacctatgtc gccaccacca cgaaatcctg ggccgggaat ggcatcgacc
     2341 cgcagagcat gatcgaggaa atcgcgaagg agcatgaata tgtggcctgc cagctggaag
     2401 gtcaacagct gttcgggctg cgcacgtcgg aatcaatgca aatctgtccc accgaggata
     2461 ggcaggggga tatgctggtc gtcagtcgtg gcaccaaggg gggtaggacc agggtgatcc
     2521 ccatcgaaac tcaggcccag tacaactggt tgaggaaggc ggccgaactg gctgccctca
     2581 cgaatcgcgg caatctgatc gagggcgacc ggtcgtataa gcaggcgcgt aaccaccgta
     2641 attatgtggc gcgcaaattc ggcatcacca aaaccgaact gggcgtggtt ccccacggtc
     2701 tgcgcgtcca gtttgccgaa gagcgctatg agttggtgac cggcgcgccg gctccgacac
     2761 gcggtggcgg ctgtgttacg ggggaggagg accggcaggg tcgcgtcgag gtgagtcgtg
     2821 tgctcgggca ctcccgcgaa agcatcgccg gggcctacat cggggccagg ccccagaacc
     2881 agcacatcgg ttagcctgat tcgtcacgaa gctcagccag gctcatccag tgagctaccg
     2941 actgcctaag ctgacgcttg tcacaagacg ggagccagcg atgcccatga accgagtcca
     3001 gttccagccc ggcgtgtcgc tgccggagtt cacggcgcgc tacggcacac aggctcaatg
     3061 tgaggccgcg ctcgcgcaga tgcgctggcc cgaggggttt cggtgtccgc gatgccagtc
     3121 ggcggagcac tatgtcgtgg gccatggcgc ccggcggctg taccagtgca agtcctgtcg
     3181 ccaccaggcg tcgctgacgg cgggcaccgt gatggacagc tccaagctgc cgttgcagac
     3241 gtggttcctg gccctgtacc tgatcagcca agccaagaca ggcctgtcgt cgctcgcgct
     3301 gatgcaccag ctaccgcacc gcttggttgc tgcaccacaa gctcatggcc gcgatggcgc
     3361 agtgcgatgc gcagcagccg ctggacggag atgtgcaact ggacgacgcc tacctgggcg
     3421 gcgagcgccc gggcgtgggc gggcgcggct cgccgaacaa ggtgccgctg gtggcggcgg
     3481 tcagcaccaa tgacaagggc cacccgatgc gggtcaagat cgcagcgatc aagacgttca
     3541 gccgcgaggc ggtcgccagc tgggcccggg cgaacctgct gcccggctgt gatgtgcgca
     3601 gcgacggtct ggcctgcttc gccggcgtca tcgatgcggg ctgcgcgcac tcgtacatcg
     3661 tcgtgggcac ccgcaagccc cgcgaactgc cgcagttcac ttgggtgaac accgtgctgg
     3721 gcaacctgaa gacgatggtg aacggcgcgc acaagtggtt ccgcttcggc aagtacgccc
     3781 aacactacct tggagccttc gcctaccggt tcaacaaccg gttcgatctg gcggccatgc
     3841 tcaccgcgac gatcaggcaa gcagccaccc gcagaccgac gcgcgagcgg caggtgcggg
     3901 gtatggctga gcttcatgac taatcaggtc ggttagtgaa gcgagcagcg cccgaggtcg
     3961 ctgccgacgc catatgcgcc aagccgccgc gcgggctgca cgccgacgtg cacgacggcg
     4021 tgccccgtgg agtagtcgta ctttccgtgc agattggtgc gctttgcttt tgagaacccg
     4081 tcgcacgaca agcacttacg attcccgcct ttccgagcac tgcgggcttt tcgtgcagat
     4141 cggtgcgctt cgtctcaggt acccctggga ggccggcccc ggcgttctca ggcgatcggc
     4201 ccgatcaaac ctcttcgctc cgatcgcgaa actccagcct gaactgccag ccctcaaagg
     4261 tcgccaccat ccgtccgaac tgttcccagg agatctcgcg accatcgatg acaaccatcg
     4321 gcacacgatg gtccgcgtcg gggtcgctgt cgacgctccc ccgcagcacc aggccatcgt
     4381 tcacctgcgg cccgtggtcg gtgtcctcca gatgcgtgag agccaatgcc cggcgcatct
     4441 tgccgatcag cttgcccagc agttccagcg ggtcgtcgtg aggctccccg atcacctgaa
     4501 accgataccc ggcaaggccg ccatcgcgca actcgaacgc gtcgatcgcc aagccggtgc
     4561 cacacaacca gttccggaac cggaactcgt gctccgcccc gcgcccgtcg agcatgcgga
     4621 tgggctcgaa cgccacgtgc tcgaacccct gcagcccgag tcggccggcg gcggcctcgt
     4681 tgtagcatcg gccgcagagc cgctggtagc cagcatccat cgacccgtag ttcacgatgt
     4741 cgtggtcggc agccggctga cggcaagctt cgcatgtgat gggcggcatg gcttgcgggg
     4801 tgtgtgaagg cgagacgact gtaggctacg accgcggcgc cgcgaaccga tcggccacct
     4861 gcttggcgcg ccgtgcatcg tcggtgtgca ggtacatcga ggtcgtggcc agccaggcgt
     4921 gccgcaggtt gtcgcgcacc gttgtcagct cggcgccacc ttggaggaag tgcgtggcat
     4981 gcgtgtgcct cgcgaagtgc ggcgtggcct gccgcaggct cgtcgatttg gactctcaac
     5041 aaggttgaga gtttcggcgg attcagcttg cggccctgta tgaggcgccc ggaagccgcc
     5101 cgtcgtggga accggccatc ggccatcagc ggccggtcgc ccggcgcccc aaactctgcc
     5161 cgcaacgagg gcgtgaatgg ccgcttagcg gaaacgagtt tgtaccgctc accggtccaa
     5221 cccggccctc agccgacatt cggaagatgc cgttttgggc ctgcggcggt tgagcatctg
     5281 ctgacgagac ggccttcgag gccgtcagtc gcacccggtg catggtcggc tggtaccagc
     5341 cgacctcgac agcgcgacgg gagtcgatac ggctcggctc ccgttcgcct catcgagcgc
     5401 gcaactttgc gtgctgatag gtcgccttct ccatggccag gaagcccacg accgacgtcg
     5461 gatcggcgaa cgggaagatc tgcgtcgccc agtatccgcc gacgccgttc gcgcgatcga
     5521 tccagtagta gaggttggcg aggccggccc atgccaatgc tccggccggc cggccggtgg
     5581 gcgcctcttc ctcgttgatc atgaaggtga gtgcccaggt cttgggcatg ccggggaaga
     5641 actccgcgtc gttggacaga cgcggtttcg acaccggcag gatcgacgcc gccgtggtgg
     5701 tcgcaccgac gccgggcagc atcttgatgt gcattccctt gggcagatga ttgcgcgacg
     5761 ccatctcgac ggtctcgggc cgcaggatct gctcgccgct gtcgctgcgc ccgtcgttga
     5821 gccacatgcg gatgaacttc acgtagtccg
//
LOCUS       DAOORV010000202         5771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16848_length_5771_cov_5.843247, whole genome shotgun sequence.
ACCESSION   DAOORV010000202 DAOORV010000000
VERSION     DAOORV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_5771_cov_5.843247"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="cysD"
                     /locus_tag="PKV56_16985"
     CDS             complement(<1..590)
                     /gene="cysD"
                     /locus_tag="PKV56_16985"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOX69551.1"
                     /translation="MKLTHLQRLEAESIHILREVVAETENPVMLYSVGKDSATMLHLA
                     RKAFYPSPPPFPLLHVDTTWKFRDMYALRERMVSETGMQLLVYQNPEAIERGINPFDH
                     GSQIHTDMWKTQGLKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRSAQHRWDPKFQ
                     RPELWQLYNARISKGESIRVFPLSNWTELDIWQYIYL"
     gene            1568..2335
                     /gene="cysQ"
                     /locus_tag="PKV56_16990"
     CDS             1568..2335
                     /gene="cysQ"
                     /locus_tag="PKV56_16990"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOX69552.1"
                     /translation="MTDAELLESVTQIARAAGELILRVYSTDFSVRGKGDASPVTEAD
                     EQAESLILAALGELTPNTPVVAEESVAAGCVPDIGDGSGRFWLVDPLDGTKEFISRNG
                     EFTVNIALIERGEPVLGVVLAPALGRLFAGSRGNGAWVQDQSGRRSIRCRAIPEAGAT
                     VVASRSHGDAAALDAFLSGTKVANLVSAGSSLKLCLVASGEADMYPRLGRTMEWDIAA
                     GHAVLAAAGGRVQRLDDGQPLLYGKPGFDNPHFVAAG"
     gene            2660..4378
                     /locus_tag="PKV56_16995"
     CDS             2660..4378
                     /locus_tag="PKV56_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOX69553.1"
                     /translation="MSAAELAAPANSTELRAPLRPRLTLGNPRFTNSQLVALAREQGN
                     PAAWAAALTQGPAKAAAAAEGDFAVGLTDHTGSTFLAVDRFAVCSICYRVIDGQLHFA
                     ERADELAKLAPTAAVSPQAIFDYLYFHVVPSPRTIFEGVYRLPAGHYAWFESGKLTVA
                     PYWIPEFHEPASPSFEPLASEFRDLLRQAVATQLDGSKPACFLSGGTDSSTVAGMIGL
                     ATGRPAASYSIGFEAEGYDEMEFARIAAKRFGTEHHEYYVTPADLVRGIPAVAVHYDQ
                     PFGNSSALPAFYCARMAREDGVTRLLAGDGGDELFGGNTRYAKQRVFGYYDGVPAALR
                     SRVLEPLLEHTPLGKLPLARKARSYVEQAKVPLPDRMQMYNLLLRLGPEQVFTTAFLD
                     RIDQADPLRQQQAVWKQLPDTSPLNRMLAYDWRYTLAEADLPKVCGTTALAGVAAGFP
                     LLDQRLVDFSLRLPTEYKLNGLKLRWFFKEALRGFLPDEILTKKKQGFGLPFGVWANR
                     DEKLREFASESLRSLGRRGIIQPAFIDALLKQHLPAHPGYYGEMVWILLMLEQWLRGH
                     SPDVTA"
     gene            4444..5262
                     /locus_tag="PKV56_17000"
     CDS             4444..5262
                     /locus_tag="PKV56_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX69554.1"
                     /translation="MLRVAKNIYDYRGLMATLAWKNIAIRYKQAYLGIAWAVIKPLTL
                     MLIFTLVKTFVGIDSGTIPYPVLTFAALMPWIFFQESASEGVNSVVANTALIKKIYFP
                     REIFPITSVATKMLELGINFLILAALMIWYGMLPTAYAVWVPLIIAYTVLAALTVSFF
                     GAALNVYFRDVGQALPVLLSLVMYASPVIYPLQLVKDKLLVQQAAGAWSDRLYTLYTL
                     NPLAGIIDAFQRVVLRGQPPDFVSMLPGAALVGVLLPLSYLYFKRAESYFADVI"
     gene            5281..>5771
                     /locus_tag="PKV56_17005"
     CDS             5281..>5771
                     /locus_tag="PKV56_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX69555.1"
                     /translation="MPVIEVEHLTKEYQLGALQGLKQSVLNAGARLLGRPVEPRPLFK
                     ALDDVSFSIEQGEVVGIIGHNGAGKSTLLKMLARISTPTRGSVRVHGRIAPLIEVGAG
                     FVPDFTGRENVYLNGSILGMSRKEIDRKFDEIVDFAEMAEFIDTPVKRYSSGMQVKLA
                     FAVA"
BASE COUNT         1062 a         1870 c         1775 g         1064 t
ORIGIN      
        1 aggtagatgt attgccagat atcgagttcg gtccagttcg acagcgggaa cacgcggatc
       61 gactcgccct tgctgatgcg cgcgttgtag agctgccaga gctcgggccg ctggaacttg
      121 ggatcccagc gatgctgcgc gctgcggaac gagaagatgc gctctttcgc gcgtgacttc
      181 tcttcatctc gcctcgcccc gccgaaggcg gcatcgaatc cgtacttgtc cagcgcctgc
      241 ttcaggccct gcgtcttcca catgtcggtg tggatctgcg aaccgtggtc gaacgggttg
      301 atgccccgtt cgatggcttc cgggttctgg tagaccagca actgcatgcc cgtctcgctg
      361 accatgcgtt cccgcagcgc atacatgtcg cgaaacttcc aggtggtatc gacatgcagc
      421 agcgggaacg gcggcggcga gggatagaag gccttgcgcg ccaggtgcag catcgtggcg
      481 ctgtccttgc cgaccgagta cagcatgacc gggttctcgg tctccgcgac gacttcacgg
      541 aggatgtgga tgctttcggc ttcaagccgc tgcaggtgag tgagtttcat gggcgcagat
      601 ccggttgagg ccggttgttg aatcttcatg aatgggttcg caacatcctt gaccgccgcc
      661 tggactggcc gaccccggcg aggagtcgga cctgccgggc aggcttcgat gaccagttcg
      721 ggatggcact tgagccagtc gaccaggccg cccgtaccaa gatcgacgcc gtgcatgagc
      781 acgtgtctgg catcgtccca ggtggccagg aggcgctgca ggaatccggt gtacgactga
      841 atgctgtttg cgtcaggaat cgggagccag gacacgctgc cgcgcagcgt ctgggcgacg
      901 agcaggcggc gaggactggc gccgacaccg atgcggatct gcccgcacca gacgatcgtc
      961 gcaccgcgct tgcgggcctc gcgggtcaca ccggacaggc gctcactgcg ccagccagcc
     1021 aggccgacgt ggttttcgga cagctcggac atggacatca gcccgagtcc ggcgcgttcc
     1081 agcattcggg acagtgctcg cccctgaggt gcgtggccga agcgaatctc agcgaattga
     1141 cgcacggcct cgacagtcca caaaccgaat ccgcagccga ccgcttcggg gggagcctcg
     1201 aacatgactc gctgcaactc ggactcttcg ccctcggtga gcgaacgacg ctggcccgac
     1261 ctgcgcccca gggaatgaat ggcaacaccg gaccagccct ggctcaagaa cagcctgtaa
     1321 gcgccgatga ccgtgggggc actgaggccg gtctcgatac gtatctgctg cagcgtcctg
     1381 cccgccaatc gcagctgaac cgcacggcgc cgccgttcgt tcaacgtcaa cagtgccgac
     1441 gatgggcgct ccttcaattc agctccgatt tgttcggcga gacgcattgc aactcgtgac
     1501 tggtaaacct ttaacagacc atacattagt aggcgggtat tgaagccccc attcaaggaa
     1561 gtcacccatg accgacgctg aattactcga atccgtcacg cagattgcgc gtgcggcagg
     1621 cgagctcatc ctgcgcgtgt actcgacgga tttctctgtc cgcggcaagg gcgatgcctc
     1681 accggtgacc gaagccgacg agcaggccga atcactcatc cttgcagcac tgggtgaact
     1741 gacccccaac acgcccgtgg tggcagagga atcggtcgcc gccggatgcg tgcccgacat
     1801 cggcgatggg agcgggcggt tctggctcgt cgatccgctt gatggaacca aggaattcat
     1861 cagtcgaaac ggcgaattca ccgtgaacat cgcgttgatc gagcgaggag aaccggtgct
     1921 gggcgtcgta ctggcgcccg cgctgggccg actgttcgcg gggtcccgcg gcaacggggc
     1981 ctgggtgcag gatcaatcag ggcgcaggag catccgctgt cgagccatac ccgaggctgg
     2041 agcgaccgtc gtcgccagcc ggtcgcacgg tgacgctgcc gccctcgacg cctttctgtc
     2101 gggcaccaag gtcgcgaatc tggtttcggc aggctcctcg ctcaagcttt gcctggtggc
     2161 cagcggcgag gcagacatgt acccccgcct gggtcgcacg atggagtggg acatcgcagc
     2221 cgggcacgcg gtgcttgctg cggcgggagg ccgtgtgcaa cgactcgacg atggccagcc
     2281 cctgctttac ggcaagccgg gttttgacaa cccgcatttc gtggctgccg gctgacgcgg
     2341 atcaacttca attcgacagc accacctgtt ttgtctggcc gcctgactgc tttcaggatg
     2401 cgcttttcgc ggatttgtcg accaaagcaa ggtttcagca cacaacaagc gcgaacacga
     2461 caccaaactc agcgtgttgc gccagtacga tccgacacat attcagaaag ccatctcgaa
     2521 gattcggccg ccagcagcgg tgtaaccaca gcaccggcgg ttccggcaca ccacttctgc
     2581 ggactgccgc cgtctggcgc atcagaagac gacaccgccc agaactcatg tgcgggacat
     2641 acccaatgaa caacctgcca tgagcgccgc cgaactcgcc gccccagcca attccacgga
     2701 gctgcgggcc cccttgcgtc cacgactgac gcttggcaac ccgaggttca ccaacagcca
     2761 gctggttgcc ctggcccggg aacaaggcaa cccagcagca tgggcggccg cgctgaccca
     2821 agggccagcg aaggctgccg ccgccgcaga aggtgacttt gcggtcggct tgaccgacca
     2881 cacgggaagc acctttctcg cggtggaccg gtttgccgtc tgctcgattt gctaccgggt
     2941 catcgatggt cagttgcatt tcgcagagcg cgccgacgaa ctcgccaagc ttgcaccgac
     3001 ggcggcggtg agcccgcagg ccatattcga ttacctgtac ttccacgtgg tgccgtcccc
     3061 acgcaccatc ttcgagggcg tctatcgcct gccggcgggc cattacgcct ggttcgaatc
     3121 cggcaaactg accgtcgcgc cgtattggat tccggagttc cacgagccgg caagtccttc
     3181 attcgaaccc ctggcttcgg agttccgcga cttgctgcgc caggccgttg ccacccaact
     3241 ggacggaagc aagccagcct gcttcctgag tggcggaacc gacagctcga cggtcgccgg
     3301 gatgatcgga ctcgcaaccg gccggccggc agccagctac tcgatcggtt tcgaggctga
     3361 aggctacgac gagatggaat tcgcacgtat cgctgccaag cgattcggga cggaacatca
     3421 cgagtactac gtgacgcctg ccgatctggt gcgcggcatc cccgcggtgg ccgtgcacta
     3481 cgaccagcca ttcggcaact cgtcagcgct gccggcgttc tactgcgcca ggatggcgcg
     3541 cgaggatggc gtgacccggc tgctcgccgg cgatggcggc gacgagctct tcggtggcaa
     3601 cacccgctac gccaagcaac gtgtgttcgg ttactacgac ggcgtacccg ctgcgctgcg
     3661 cagccgcgtg cttgagcctc tcctcgagca caccccgctc ggcaaattgc cgctggcacg
     3721 caaggcgcga agctacgtcg aacaggcgaa ggtgccgctt ccagatcgca tgcagatgta
     3781 caacctgctt ctgcgtctgg gcccggagca agtcttcacg actgcctttc tggaccgtat
     3841 cgatcaagcc gacccgctgc gacagcagca ggcagtctgg aagcagctgc ccgacacgag
     3901 cccactgaac cgcatgctcg cctacgactg gcgttacacg ctggccgaag ccgacctgcc
     3961 caaagtctgc ggcaccaccg cgctggcggg cgtggctgca gggtttcctt tgctggacca
     4021 gcggctggtc gacttctcgc tgcgcctgcc caccgaatac aagttgaacg ggctgaagct
     4081 gcgctggttc ttcaaggagg cactgcgcgg gttcctgcct gacgagatcc tcaccaagaa
     4141 gaagcaaggg ttcgggctgc cgttcggagt gtgggcaaac cgggacgaaa agctgcgcga
     4201 gttcgccagc gaatccctgc ggtcgctggg tcggcgcggc atcatccagc cggcgttcat
     4261 cgatgccctg ctgaagcagc atctgcccgc acaccccggc tactacggcg aaatggtgtg
     4321 gattctcctg atgctggagc aatggttgcg aggacattca cctgatgtca ctgcatgacc
     4381 tgaccagaag gcggttgatg ccacaccgtc ccgtaaaccg gccgactaga agagcttgac
     4441 accatgctga gagttgccaa gaacatatac gactaccgcg ggctgatggc caccctggct
     4501 tggaagaaca ttgccatccg ctacaagcag gcctatctgg gcatcgcctg ggccgtcatc
     4561 aagcccctga cgctgatgct catcttcacg ctggtgaaga ccttcgtcgg catcgacagc
     4621 ggcaccatcc cctaccccgt actcacgttc gcggccctca tgccctggat cttctttcag
     4681 gagtcggcct ccgagggagt caacagcgtg gtcgccaaca cggcgctgat caagaaaatc
     4741 tacttcccgc gcgagatctt tccgatcacg tccgtggcga cgaagatgct cgaactgggg
     4801 atcaacttcc tgatcctggc cgcgctgatg atctggtacg gcatgctgcc caccgcctat
     4861 gcagtttggg taccgctgat cattgcctac acggtgctcg ccgccctcac cgtctcgttt
     4921 ttcggcgcag cgttgaacgt gtactttcgc gacgtgggtc aggccctccc ggtgctgctc
     4981 tcgctcgtga tgtatgcctc cccggtgatc tatccgctgc aactggtcaa ggacaagctg
     5041 ctcgtgcagc aggcggcagg cgcatggtcc gatcggctct acacgctcta cacgctgaac
     5101 cccctggcgg gcatcatcga tgcgttccag cgcgtggtgc tgcgcggcca gccgcctgac
     5161 ttcgtctcca tgctgcccgg ggccgcgctg gtcggtgtgt tgttgccact gagctatctc
     5221 tatttcaagc gcgcggaaag ctatttcgcc gatgtcatct gaacgaggtc actgcacacc
     5281 atgcccgtca ttgaagtcga gcacctgacc aaggaatacc agctgggcgc gctgcagggc
     5341 ctgaagcagt cggtgctgaa tgctggcgcg cggctgctgg gccgccccgt cgagccgcgc
     5401 cccctgttca aggcgctcga cgacgtgagc ttttccatcg agcaaggcga ggtggtgggc
     5461 atcatcggcc acaacggcgc gggcaagtcc accttgctca agatgctcgc caggatcagc
     5521 acgcccaccc gtggctcggt cagggtgcat ggccgcattg ccccgctgat cgaggtgggc
     5581 gccggcttcg tgcccgactt caccgggcgc gagaacgtct acctcaacgg cagcatcctg
     5641 ggcatgtcgc gcaaggagat cgaccgcaag ttcgacgaga tcgtcgactt cgcggagatg
     5701 gccgagttca tcgacacccc cgtcaagcgc tacagctcgg gcatgcaggt caagctggcc
     5761 ttcgccgtgg c
//
LOCUS       DAOORV010000203         5743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16967_length_5743_cov_7.320499, whole genome shotgun sequence.
ACCESSION   DAOORV010000203 DAOORV010000000
VERSION     DAOORV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16967_length_5743_cov_7.320499"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /gene="rpoB"
                     /locus_tag="PKV56_17010"
     CDS             complement(<1..1521)
                     /gene="rpoB"
                     /locus_tag="PKV56_17010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOX69556.1"
                     /translation="MAQHATPYSYTERKRIRKSFGKRESVLKVPYLLTMQQESYIAFL
                     QKDVPPQKRKPEGLQAAFLSAFPIVSHNGFVEMKFIEFNMAKPAFDTRECQVRGLTYA
                     AAVRAKLQMIIYDRESPQAKTVKEIKEQEVYMGEVPLMTDYGSFIVNGTERVIVSQLH
                     RSPGVFFEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDILYFRVDRRRKMPVTI
                     LLKAIGLNPESILAHFFVFDNFRLMDSGAQMEFVADRLRGEIARFDITDKNGNVVVEK
                     DKRITARHTRAIETSDTTHISVPEDYLVGRMLARNVVDADTGEILAKANDELTEAVLK
                     KLRVAGVAEIPCLYTNELDEGGYISQTLAADETADQLAARVAIYRMMRPGEPPTEDAV
                     EALFHRLFYNADTYDLSRVGRMKFNARVGRDSAEGAMTLNNEDILDVVKILVELRNGR
                     GEVDDIDHLGNRRVRCVGELAENQYRSGLARIEKAVKERLGQAETEALMPHDLINSK"
     gene            complement(1862..2239)
                     /gene="rplL"
                     /locus_tag="PKV56_17015"
     CDS             complement(1862..2239)
                     /gene="rplL"
                     /locus_tag="PKV56_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOX69557.1"
                     /translation="MAFDKDAFLTALDSMSVMDLNDLVKAIEEKFGVSAAAMSAPAAG
                     GGGAAAAVVEEKTEFNVVLLEAGANKVSVIKAVRELTGLGLKEAKDLVDGAPKNVKEA
                     VPKADAEAALKKLVEAGAKAEVK"
     gene            complement(2278..2811)
                     /gene="rplJ"
                     /locus_tag="PKV56_17020"
     CDS             complement(2278..2811)
                     /gene="rplJ"
                     /locus_tag="PKV56_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOX69558.1"
                     /translation="MSLNRNEKAAVITDVAAQVARSQTLALAEYRGLTVDHLNNLRRQ
                     AREKGVYLHVLKNTLARRAVAGTPFEVASESMVGPLIYGFSEDAVAAAKVISDFAKSN
                     DKLIVKGGAYAGKALNADGVKSLAAIPTKEVLLSQLLGLMQSPVSRIARVLAAIAEKR
                     TEPAAESAPAAEAAPAA"
     gene            complement(3081..3791)
                     /gene="rplA"
                     /locus_tag="PKV56_17025"
     CDS             complement(3081..3791)
                     /gene="rplA"
                     /locus_tag="PKV56_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOX69559.1"
                     /translation="MAKLTKRQKALVGKVETTKLYTIDSALALVKECATAKFDESIDV
                     AVQLGIDAKKSDQVVRGAVVLPNGTGKTKRVAVFAQGAKAEEAKAAGADIVGMEDLAE
                     QIKAGVMNFDVVIASPDTMRIVGTLGQVLGPRGLMPNPKVGTVTPDVAQAVRNAKAGQ
                     VQFRVDKGGIVHSTIGRRSFESDKLVGNLRALIEALNKAKPASSKGVYLRKVAVSSTM
                     GVGARVDVASINAAAPGA"
     gene            complement(3794..4225)
                     /gene="rplK"
                     /locus_tag="PKV56_17030"
     CDS             complement(3794..4225)
                     /gene="rplK"
                     /locus_tag="PKV56_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOX69560.1"
                     /translation="MAKKIIGFIKLQIPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLMLPVVITAFADKSFTFVLKSPPATVLIKKALKLDKGSPKANSQKVGKIT
                     RAQLEEIAKIKVKDLTAADMDAAVRTIAGTARSMGVTSEGV"
     gene            complement(4394..5023)
                     /gene="nusG"
                     /locus_tag="PKV56_17035"
     CDS             complement(4394..5023)
                     /gene="nusG"
                     /locus_tag="PKV56_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOX69561.1"
                     /translation="MNEETVSAEAGSPVDPVLANGEGSAPVAPAVPSNKRWYVVHAYS
                     GMEKAVERNLRERIDRSELQDKFGRILVPMEEVVELKNGKKAVTERRFFPGYVLVEME
                     MADDSWHLVKHTNKVTGFVGGAKNRPSPISEAEVMKIVHQMQEGVEKPKPKTLWSVGE
                     LVRIKDGPFTDFNGAIEDVNYEKSKVRVAVTIFGRATPVELDFAQVEKV"
     gene            complement(5034..5417)
                     /gene="secE"
                     /locus_tag="PKV56_17040"
     CDS             complement(5034..5417)
                     /gene="secE"
                     /locus_tag="PKV56_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOX69562.1"
                     /translation="MANPTVETVSTGADKAKLAGVVLLVVGAVAAFYALDKQDLWLRV
                     IALLALIAVAVAVFFTSEPGKELIAYGRDSIKEVRKVVWPTRQEAMQMTGYVFAFVVV
                     MALFLWLTDKTLEWVFYDLLLGWKR"
     gene            complement(5575..5650)
                     /locus_tag="PKV56_17045"
     tRNA            complement(5575..5650)
                     /locus_tag="PKV56_17045"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5615..5617),aa:Trp,seq:cca)
     gene            complement(5667..>5743)
                     /locus_tag="PKV56_17050"
     CDS             complement(5667..>5743)
                     /locus_tag="PKV56_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOX69563.1"
                     /translation="EGLRFAIREGGRTVGAGVVAKIIE"
BASE COUNT         1042 a         1829 c         1670 g         1202 t
ORIGIN      
        1 cttggaattg atcaggtcgt gcggcatcag ggcctcggtc tcggcctggc cgaggcgttc
       61 cttcacggcc ttctcgatcc gggccagacc actgcggtac tggttctcgg ccagttcacc
      121 gacgcaacgc acgcggcggt tacccaggtg gtcgatgtca tcgacttcac cgcggccgtt
      181 gcgcagttcg acgaggatct tgacgacgtc gaggatgtcc tcgttgttca gcgtcatcgc
      241 gccttcggcc gaatcacggc cgacgcgggc gttgaacttc atgcggccga cgcgcgacag
      301 gtcataggtg tcggcgttgt agaacaaacg atggaacagc gcttcgaccg cgtcttcggt
      361 cggcggctcg ccggggcgca tcattcggta gatggccacg cgcgcagcca actggtctgc
      421 ggtctcgtca gcagccaggg tctgcgagat gtagccgcct tcgtcaagct cgttggtgta
      481 caggcaaggg atctcggcga caccggccac gcgcagcttc ttcagcaccg cctcggtcag
      541 ctcgtcgttg gccttggcga ggatctcgcc ggtgtcggca tcgacgacgt tgcgcgccag
      601 catgcggccc acgagatagt cctcgggcac gctgatatgg gtcgtgtccg atgtctcgat
      661 ggctcgggtg tggcgtgccg tgatgcgctt gtccttctcg accacgacat tgccgttctt
      721 gtcggtgatg tcgaaacggg caatctcacc gcgaagccgg tcggcgacga actccatctg
      781 cgcgccggaa tccatcaggc ggaagttgtc gaagacgaag aagtgcgcaa ggatcgattc
      841 cgggttcagc ccgatggcct tcagcaggat cgtcaccggc atcttgcgac ggcggtctac
      901 gcggaagtac aggatgtcct tggggtcgaa ttcgaaatcg agccacgatc cgcgataggg
      961 gatgatccgc gcgctgaaca acagcttgcc gctcgagtgt gtcttgccct tgtcgtgctc
     1021 gaagaacacg ccaggggaac ggtgcagctg cgacacgatg acgcgctcgg ttccgttgac
     1081 gatgaaagag ccgtaatcgg tcatgagcgg cacttcgccc atgtagacct cctgctcctt
     1141 gatctccttg accgtcttcg cctggggaga ctcgcggtca tagatgatca tctgcaactt
     1201 ggcgcgaacc gcagccgcgt aggtcaagcc acgcacctga cactcccgcg tgtcgaatgc
     1261 cggcttggcc atgttgaact cgatgaactt catctcgacg aacccgttgt gcgacacgat
     1321 cgggaacgcg gagaggaaag cggcttgcag cccctcaggc ttgcgcttct gcggcggtac
     1381 atccttttgc aggaaggcga tgtacgactc ctgctgcatg gtcaggaggt acgggacctt
     1441 gaggacgctt tcgcgttttc cgaagctctt gcggatccgc ttgcgctcgg tgtagctgta
     1501 gggggttgcg tgctgcgcca tgtgtggttt tccactccgt ctcaaagagc gcgacctggc
     1561 ctaggcctcg ctctcatcgg cgactgtcgt tcgaaccctg tctcgcggga caaggccatt
     1621 cgaagcttgg tggttggcct ctaccaaccg ctggcggaca accccggcat tgcaccggag
     1681 tccgaccaaa cccgttctct gcagtcggat cagagaacta ttttgaaatc acttgggaag
     1741 accctgattg tcaccgcagg attctctcaa gtgatctcca agggctatct tttcagacgg
     1801 cgcttgaagc gcaaagggcc gggcgcctga aacagcgccc agcccctggc gggaagttga
     1861 attacttcac ttcggccttg gcaccagctt cgaccagctt cttcagagct gcttcggcgt
     1921 cggccttggg cacggcttcc ttgacgttct tcggtgctcc gtcgaccagg tccttcgctt
     1981 ccttcaggcc gagacccgtc agttcgcgaa cggccttgat gacggaaacc ttgttcgcgc
     2041 ctgcttcgag cagcacgaca ttgaactcgg tcttctcttc aacgaccgct gcggccgcgc
     2101 cgccaccgcc agcggccggg gccgacattg cagctgcgga cacgccgaac ttttcttcga
     2161 tggccttgac caggtcgttg aggtccatca ccgacatgct gtcgagtgcg gtcaggaatg
     2221 cgtctttatc gaatgccatg ttgaggctcc tagattcagt gaattatcaa gaatgggtca
     2281 agcggctggt gcagcttcgg cggcaggggc cgactcagca gcaggctcag tgcgcttctc
     2341 ggcaatggcc gccagcacgc gtgcgatgcg cgagaccggc gattgcatca aaccaagcaa
     2401 ctgcgacagc agcacttcct tggttggaat ggcggcgagg gatttgaccc cgtcggcatt
     2461 cagcgccttg ccggcgtacg cgccgccctt gacgatcagt ttgtcgttgc tcttggcaaa
     2521 gtcggaaatg actttggccg cagcaactgc gtcttctgaa aaaccataga tcagcggacc
     2581 gaccatgctc tccgaggcca cttcgaacgg cgtgccggcg acagcgcgac gagcgagcgt
     2641 gttcttcaag acatgaaggt acacaccctt ttcgcgcgcc tgacggcgca ggttgttcaa
     2701 gtgatctacg gtgaggccac ggtattcggc cagcgccagc gtctgcgagc gtgccacctg
     2761 ggccgccaca tccgtgatca cggctgcttt ctcgttgcga ttgagactca aggtctgctc
     2821 ctcacatcac gcacccttcg ctctttcgag cagccgggtg caccggggtt cagcgaccat
     2881 ctgtccagga aatcaatcct ttacagcggg accgccatct gcgctggctt gttgccttcc
     2941 tcggctctca ccgagtctga ccttccatta agcgatcgtt ggatcgcacc agcggtcttg
     3001 gatgacctgc ttgcggattg ctccggtcac agcccaccaa actcatgtgc cgacgcctct
     3061 cggcaccggc ctcttgtcac tcaggcgcca ggcgcagcgg cgttgatcga ggccacgtcg
     3121 acgcgggcgc ccacacccat cgtcgatgaa acagccacct tgcgcaggta cacgcccttg
     3181 ctcgatgctg gcttggcctt gttcagcgct tcgatgaggg cgcgcagatt gcccaccagc
     3241 ttgtccgact cgaacgagcg gcggccgatc gtgctgtgca caatgccgcc cttgtcgacg
     3301 cggaactgca cctgacccgc tttggcattg cggaccgctt gcgcaacgtc aggcgtcacg
     3361 gtgccgacct tcgggttcgg catcaggccg cgcgggccca gaacctgacc cagcgtaccg
     3421 acgatacgca tcgtgtcggg cgaggcgatc accacgtcga agttcatcac gccggccttg
     3481 atctgctcgg ccaggtcttc catgccgacg atgtcggcgc cagccgcctt ggcttcttct
     3541 gccttggcgc cctgggcaaa cacggcgacg cgcttggtct tgcccgtgcc gttgggcagc
     3601 acgacggcgc cacgcacgac ctggtcggac ttcttggcat cgatgccgag ctgcaccgcc
     3661 acgtcgatcg attcatcgaa cttggctgtg gcgcactctt tcaccagcgc gagagcgctg
     3721 tcgatggtgt aaagcttggt cgtttcaacc ttgccgacca gagccttctg gcgttttgtc
     3781 agtttggcca tggtcagaca ccctccgaag tgacacccat cgagcgggca gttccagcaa
     3841 tcgtgcgaac ggcggcatcc atgtcggcgg ccgtcaggtc cttgaccttg atcttggcga
     3901 tctcttcgag ttgagcacgc gtgatcttgc caaccttctg cgaattggcc ttcggagagc
     3961 ccttgtcgag cttcagcgcc ttcttgatca gcacggttgc cggcggtgac ttcagaacga
     4021 aggtgaacga tttgtcggcg aacgcggtga tcaccactgg cagcatcaga ccgggttcga
     4081 caccttgggt ctgggcgttg aacgccttgc agaactccat gatgttgaga ccgcgctgac
     4141 cgagtgccgg gccgatcggg ggggaggggt tggctttgcc tgcaggaatc tgcagcttga
     4201 tgaagccgat gatcttcttt gccatgtcta tccttcaatc agaagtccga taaatctgga
     4261 ctcctcgagt gcaaacgcca gggactggtt acccgggctc ctcatgccga tgtctcgtca
     4321 tcggcagcaa cgagcgagat ggacccgggc cttgcgacac gggagagctc tcgccgagct
     4381 ctctatttcc tcgtcagacc ttctcgacct gcgcgaaatc cagctcgacc ggagttgcgc
     4441 ggccgaagat cgtcaccgcg acgcgaacct tcgacttctc gtagttgacg tcctcgatcg
     4501 caccgttgaa atcggtgaac gggccgtcct tgattcgaac cagctcaccg accgaccaca
     4561 gggtcttggg cttcggcttc tcgacgccct cctgcatctg gtgaacgatc ttcatcacct
     4621 cggcttcgga aatcggcgat ggccggttct ttgcaccgcc gacgaacccg gtgaccttgt
     4681 tcgtgtgctt gaccaggtgc cagctgtcgt cggccatctc catctcgacc agcacatagc
     4741 ccggaaagaa gcgacgctcc gtcacggctt tcttgccgtt cttcagttcg accacttcct
     4801 ccatcggcac caggatgcga ccgaacttgt cctgcaactc ggaacggtcg attcgctcgc
     4861 gaaggttgcg ctcgaccgcc ttttccatgc cggaataggc gtgaacgaca taccagcgct
     4921 tgttcgatgg cacagcgggc gccacaggag cagacccctc cccgtttgcc aggacgggat
     4981 cgaccggcga accagcctct gcgctgacgg tttcttcgtt catggtgtga tcctcaacgc
     5041 ttccaaccca acagcaggtc gtagaaaacc cattccaggg tcttgtcggt caaccacaag
     5101 aacaaggcca tgaccaccac gaaggcgaac acgtagccag tcatctgcat cgcctcctgg
     5161 cgagtcggcc acaccacctt gcgcacttcc ttgatcgaat cacgcccata ggcgatcaat
     5221 tccttgcccg gctcggaggt gaagaacacg gcgaccgcaa cggcgatcaa cgccagcaac
     5281 gcgatgacac gcagccagag gtcctgcttg tccagcgcat aaaacgcagc caccgctccg
     5341 accaccagca acaccacacc cgccagcttg gccttgtcgg cgccggtcga taccgtctct
     5401 acggttggat tggccatgtc gttctattca cctcgaaaac ctgtatgcca cgcgccgcaa
     5461 tccatgagca aagcccgcaa gcacttttga ggtgccagcg ggccgctttc ggcacgacgc
     5521 ttctcggtca gcgtttcatt tcaatcccgc caccagccac caatcaccgg cggatggcag
     5581 gggcggaggg catcgaaccc ccaacctctg gttttggaga ccagcgctct gccaattgag
     5641 ctacgcccct atcgccgact tcaaactcac tcgatgatct tggcgacgac gccggcgccg
     5701 acggttctgc caccttcacg gatggcgaag cgcaggccct ctt
//
LOCUS       DAOORV010000204         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_16996_length_5732_cov_8.116259, whole genome shotgun sequence.
ACCESSION   DAOORV010000204 DAOORV010000000
VERSION     DAOORV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16996_length_5732_cov_8.116259"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2039)
                     /gene="traC"
                     /locus_tag="PKV56_17055"
     CDS             complement(<1..2039)
                     /gene="traC"
                     /locus_tag="PKV56_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HOX69564.1"
                     /translation="MLESLRLNLFGSAESAGASDRSGPGLFSFGHKDESPADQFASWL
                     PYISYLDEEQLFVNKDGMGFMLEVMPQSGADDRMVDVLVSLYSNCPPDTGIQFHLFAS
                     PHICPPLRKYANLRMEDRDQIQKAQAFGRPARNDNLYRKLARRRYAHLSHGAHHSLTQ
                     GFHYTIRDFRLMVSVSVKADPGNLSDRERIVGLRDGMGTTLRSASLPNRVCDAADLIN
                     WCSLFVNPDRIDAATAPDLHYDDGRELRDQIVDHDTIQDARSNGLHLWKEGAGDGLDV
                     RFFSIKSFPERFGLWQMGALVGDLMQPALQYSSPFLLTMGVYILDPNSTKTVVTANHV
                     RATQNAGSKMAVVMPDVAKKARDWSAAADAIDTGGALVSMYHQLGLFCHPAKVAQTQE
                     TAKAIWRARGFELNADVYKHRQALLASLPMTLSPAFHGDLRKMKRVTRKTMANAIHLA
                     PVLAEWRGSKTPTLMFAGRRGQLLTLDLYDNDGNYNAAVIGSPGSGKSVLLNELAWSY
                     RAIGAKIWMQDLGRSFEKLCRKADGTFIEFRPDVEISLNPFPLVTDINEDIDMLLPAI
                     AKMASMKQLDEVQMKAISAMLLKLWAKYGQELDMTILRDSFVPGTIEELGIKDDQRVR
                     DLAVMLNPYAKGGQYARFFSGRNTIDFSNDFVVIESEELKRKPDLQAVVNIILFHA"
     gene            complement(2057..2683)
                     /locus_tag="PKV56_17060"
     CDS             complement(2057..2683)
                     /locus_tag="PKV56_17060"
                     /inference="COORDINATES: protein motif:HMM:NF021212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HOX69565.1"
                     /translation="MSSRFFVPTGCVLAIAALLQGCAFSGLDGDSKYACKAPEGVRCD
                     SVSGTYYNALQNNLPAQRRSAPPVDTSPQSAPPAGTPRAAPAMLNASTRPAAGDEGAA
                     YVAAPLRAAPRVLRLWIKAWEDADRDLVGESLVYVQVDNGRWLVDHVQRQQRDAFMPI
                     RAPRPPAATKSAGAAAGPNGPSSVPTEDAPSLTQALRALQGRTPAADQ"
     gene            complement(2680..3492)
                     /locus_tag="PKV56_17065"
     CDS             complement(2680..3492)
                     /locus_tag="PKV56_17065"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOX69566.1"
                     /translation="MKNRCVLAGPVLALALQSALANTSADEARLLATLKRTYPATTFT
                     SVAASEVPGIYEVWMGPNVAFVSAKNPRYFIMGRLLDAKTATDITGPKLARAQSARPA
                     SETMEGADKPIDIGRLPLQDALKVVHGTGARTLYAFSDPACSFCRRLEPELAKLQDVT
                     VYTFLLPFQGRQLPQAVLCSPDPVKSWQAVMLNADTTGLNSQAECATPLERNLQLARQ
                     LGVNGTPTIFYADGTRTTGYVPGAEVERRIAAAAASRAPVANAQAITQEKAP"
     gene            complement(3482..4885)
                     /locus_tag="PKV56_17070"
     CDS             complement(3482..4885)
                     /locus_tag="PKV56_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HOX69567.1"
                     /translation="MPAFVDNLRERWTGITERMSPRARQWTVAAVICGGGVALLWAVF
                     SAGSGDNAQRAAAAAAKSEVRPTNVDLMAPGSQVKDSEVWIGKAGKELESYKAERDEQ
                     RKVNRDLTDKQQELLRRLAEMEERLKTRPLDAAQAASAPTTAAAPAKPALANPPEAAL
                     PRAPGTLPPPPPPPVAYQRGAMPPGLPGTSSPSGIDVAAAPVQPQLMRVTLAAPARAS
                     DAAEGGGKKPRETLDSFLPISFTKGQLLGGLDAPTGGQAQSNPHPVLIRLSDNSVLPN
                     RYRAEYRECFVIAAGYGDISSERAYLRTELLSCVRPNGDPLEVKIQGSVFGEDGKVGM
                     RGRLVTKQGQMLANALLAGVVSGIGQGFSQANTTYSTSPLGSVATASGSDAYRAGIGS
                     GVGKALDRLAQYYIKLAEQTFPIIEIDAGREIDVVLTKGVRIDGSDPNTASNSSTSMP
                     GRSDPAERYLKVTSDEE"
     gene            complement(4888..5718)
                     /locus_tag="PKV56_17075"
     CDS             complement(4888..5718)
                     /locus_tag="PKV56_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HOX69568.1"
                     /translation="MRNLASKFASALLIPTVLLLVSATPSHALQLVEASDGVSVEAVL
                     SIKEPTRIRVDGSSITNVFGNIYSTNCGSAITNAAPAQPGAAPMPPTGSINPAGEIVL
                     ECDVDKGEIYVRPVGGPGKPVNLFVSTQHATYTLLLRRSDTPADTIVIRDKTVRLSRV
                     EPGGASAAARTSQHVRSLKGMLVAMSSERAPADIRVEEVNRPIQLWQEAKFALVRQYE
                     GRGLIGERYLLTNVSSQDMVLAEQEFDRERGNVLAISIENHNLRPGESTTVYVIRQGT
                     "
BASE COUNT          921 a         1754 c         2001 g         1056 t
ORIGIN      
        1 gcgtggaaga ggatgatgtt gacgaccgcc tgcaggtcgg gctttcgctt gagctcctcg
       61 ctctcaatga cgacgaagtc gttggagaaa tcgatcgtgt tgcgaccgct gaagaagcgc
      121 gcgtactggc cacctttggc gtacgggttg agcatcaccg cgaggtcgcg cacccgctgg
      181 tcgtccttga tgcctagctc ttcgatcgtt ccgggcacga aggagtcgcg caggatggtc
      241 atgtccagct cctggccgta cttggcccag agcttgagca acattgccga gatcgccttc
      301 atctggacct cgtcgagctg cttcatcgag gccatcttgg cgatggccgg gagcagcatg
      361 tcgatgtcct cgttgatgtc cgtgaccagc gggaacgggt tcagcgaaat ctcgacgtcc
      421 ggtcggaact cgatgaatgt gccatccgcc ttgcggcaca gcttctcgaa cgagcgcccg
      481 aggtcctgca tccagatctt cgcgccgatg gcacggtagg accaggccag ttcgttgagc
      541 agcaccgact tgccggagcc gggtgacccg atgaccgcgg cgttgtaatt cccgtcgttg
      601 tcatacaggt cgagggtaag cagttggccg cggcggccgg cgaacatcag ggtgggcgtc
      661 ttgctgccgc gccattcggc cagcaccggc gcgaggtgga tcgcgttggc catcgtcttg
      721 cgggtgacgc gcttcatctt gcgcagatca ccgtggaagg ccggcgacag tgtcatcggc
      781 aggctggcca gaagggcctg gcggtgcttg tagacatcgg cgttcagctc gaagcccctc
      841 gcccgccaga tggccttggc tgtttcctgc gtctgagcga ccttggccgg gtggcagaag
      901 aggccgagct ggtggtacat gctcaccagc gcgccaccgg tatcgatcgc gtccgcggcg
      961 gccgaccagt cgcgtgcctt cttggcgaca tcgggcatca ccacggccat cttgctgccc
     1021 gcgttctgcg tggcccggac gtggttggcc gtgaccacgg tcttggtcga gttggggtcc
     1081 aagatgtaga cgcccatggt cagcaggaac ggcgagctgt actgcagcgc tggctgcatc
     1141 aggtcgccga cgagcgcccc catctgccac agcccgaacc gctccgggaa cgacttgatg
     1201 gagaagaagc gcacatccag gccatcgccg gcgccctcct tccacaggtg aagtccgttg
     1261 gagcgcgcgt cctggatcgt gtcgtggtcg acgatctggt cgcgcagctc gcgcccatcg
     1321 tcgtagtgca ggtcgggtgc agttgcggcg tcgatgcggt ccggattgac gaacagggag
     1381 caccagttga tcaggtcggc ggcatcgcag acccggttgg gaagcgaagc cgagcgcagc
     1441 gtggtcccca ttccatcgcg cagaccgacg atgcgttcac ggtcggacag attgccagga
     1501 tcggccttga ccgagacgct gaccatcagg cggaagtcgc ggatggtgta gtggaaacct
     1561 tgcgtcagag agtggtgagc cccgtgggac aagtgggcgt agcggcgtct ggccagcttg
     1621 cggtacaggt tgtcgttgcg agcgggacgg ccgaatgcct gtgccttctg gatctgatcg
     1681 cggtcttcca tgcgcaggtt ggcgtacttg cgcagcggcg ggcagatgtg gggagaggcg
     1741 aagaggtgga actggatgcc cgtgtccggt gggcagttcg agtagagcga caccagaaca
     1801 tcgaccatgc ggtcgtctgc gccgctttgc ggcatgacct ccagcatgaa gcccatacca
     1861 tccttgttca cgaacaactg ctcttcatcg aggtaggaga tgtagggcag ccaactggcg
     1921 aactgatccg cgggcgattc gtccttgtgc ccgaaggaga acagtcccgg tccggacctg
     1981 tcgctggccc ccgcggactc agcgctgcca aagaggttga ggcgaaggga ctcgagcatg
     2041 gaagtgccct ccagagctat tggtcagcgg caggcgtgcg gccctgcagc gcgcgcaggg
     2101 cttgcgtgag cgacggcgcg tcctcggtcg gaactgacga aggaccattc ggtccggctg
     2161 ccgcgccagc cgatttcgta gccgcaggcg gccgaggcgc gcggatcggc ataaaggcgt
     2221 cgcgctgctg tcgctgcacg tggtcgacca gccagcggcc gttgtcgacc tgcacataga
     2281 cgaggctttc cccgacgaga tcgcggtcag cgtcttccca tgccttgatc cagagccgca
     2341 ggacacgcgg tgccgcgcgc aacggtgccg ccacataggc cgccccttcg tcgcctgcgg
     2401 ccgggcgcgt ggacgcattg agcattgccg gcgctgcacg tggcgtgccg gcgggagggg
     2461 ccgactgcgg cgacgtatcc accggtggtg cagagcgcct ctgcgctgga aggttgttct
     2521 gcaatgcgtt gtagtaggtg ccggacacgg agtcgcatcg aacgccctca ggggccttgc
     2581 atgcgtactt cgaatcgccg tcgaggcctg agaacgcaca gccctggagc aaggccgcga
     2641 ttgcgagcac acaaccagtc gggacgaaga acctgctgct catggcgctt tctcctgggt
     2701 gatggcctgt gcattcgcga ccggcgctct gctggcagcc gcagcggcta tgcggcgttc
     2761 gacctcagcg ccaggcacat agccggtggt tcgggtgccg tccgcgtaga agatcgttgg
     2821 cgtcccgttg acaccgagtt gtcgtgccaa ttggaggttg cgctcaagcg gcgtggcgca
     2881 ctcggcctgg ctgttcaggc cagtggtgtc ggcgttgagc atcacggcct gccagctctt
     2941 caccggatcg ggggaacaca gcaccgcctg gggcaactgc cgcccctgga aaggaagcag
     3001 gaaggtgtag accgtgacgt cctgcagctt ggcgagttcc ggctcgaggc gccggcagaa
     3061 cgagcaggcc gggtcgctga aggcgtaaag ggtgcgggca cccgttccgt gaacgacctt
     3121 cagggcgtcc tgcagcggaa gccttccgat gtcgatcggc ttgtctgcgc cctccatcgt
     3181 ttcggaggcc ggtcgagccg attgcgctct cgcgagtttg ggaccggtga tgtcggtggc
     3241 cgtcttcgcg tcgaggaggc ggcccatgat gaagtaccgt gggttcttcg ccgagacgaa
     3301 cgcaacgttc ggtcccatcc agacttcata gattccaggc acctcgctcg ccgcaaccga
     3361 cgtgaaggtg gtcgctgggt aggtgcgctt gagggtggcg agtagccggg cctcgtcggc
     3421 ggaggtgttg gccagggcgg attgcaacgc cagcgccagg acggggccag ccaggacgca
     3481 tctattcttc atcggaggtc accttcagat agcgctctgc cggatccgag cggccgggca
     3541 ttgaggtgga ggagttggag gccgtgttgg ggtcggatcc gtcgatgcgc acgcccttgg
     3601 tcaggacgac atcgatctct cggccggcat caatctcaat gatcggaaag gtctgctcgg
     3661 caagcttgat gtagtactgg gccaaccggt cgagggcctt gccgacccct gagccgatgc
     3721 cggcgcggta cgcatcggag cccgaggcgg tcgcgacaga ccccagaggt gaggtgctgt
     3781 acgtcgtgtt ggcctgggag aagccctgac cgattccgct gacgacgccg gccagcagcg
     3841 cgttggccag catctggcct tgcttggtca ccaggcggcc tcgcatgccg accttgccat
     3901 cctcaccgaa gacggaccct tggatcttca cttccaacgg atcgccattc ggtcgaacgc
     3961 aggacagcag ttcggttcga aggtatgcgc gctccgagct gatgtcgccg tagccggccg
     4021 caatgacgaa gcattcgcgg tactcggcgc gatagcggtt tggcaggaca gagttgtccg
     4081 acaggcggat cagcaccggg tgcgggttgc tctgggcctg gccaccggtc ggcgcgtcca
     4141 gaccaccgag cagctgcccc ttcgtgaagc tgatcggcag gaagctgtcg agcgtctccc
     4201 gtggcttctt gccgccgcct tcggctgcgt cgctggcgcg cgcgggcgcg gcaagcgtga
     4261 cacgcatcag ctgaggttgg actggcgctg cagcgacatc gatcccgctc ggcgaagagg
     4321 tgccgggcaa tcctggtggc atggcaccac gctggtaggc cacggggggt ggcggtggag
     4381 gtggcagcgt gccaggcgcg cgcggcaggg ccgcctcagg agggttcgcc agggccggtt
     4441 tggccggagc agccgctgtc gtcggcgcgg aggccgcttg agccgcgtcc agaggacgcg
     4501 tcttgagtcg ctcctccatc tccgcgaggc ggcgcagcaa ctcctgctgc ttgtcggtga
     4561 ggtcgcgatt gaccttgcgc tgttcgtcgc gttcggcctt gtagctctcg agctccttgc
     4621 cagccttgcc gatccaaacc tcggagtcct tcacctgtga acccggtgcc atcagatcga
     4681 cgttggtcgg ccggacctcg ctcttggccg cggcggctgc agcgcgctgc gcgttgtccc
     4741 cggagcccgc cgagaacaca gcccacagga gtgcaactcc gccgccgcat atgacggccg
     4801 ccacggtcca ctgccgcgcg cgcggcgaca tgcgctcggt gatgcccgtc cagcgctctc
     4861 ggaggttgtc gacgaaggct ggcatggtca ggtcccctgg cggatgacgt agacggtggt
     4921 gctttcaccg ggacgcaggt tgtggttctc gatcgagatg gccaacacgt tgccccgctc
     4981 gcggtcgaat tcctgctcgg ccaggaccat gtcctggctc gagacgttgg tgagcaggta
     5041 gcgctctccg atcaggccgc ggccctcgta ctggcgtacc aaggcgaact tcgcctcctg
     5101 ccagagctgg attggccggt tgacctcttc cacgcggatg tccgcgggcg cgcgctccga
     5161 gctcatggcc accagcattc ccttcaggga acggacgtgt tgagaggtgc gggccgctgc
     5221 gctggcgcca ccgggctcga cgcgggacag ccgaacggtt ttgtcccgga tgacgatcgt
     5281 gtcggccggc gtgtcggagc gtcgcagcag cagggtgtag gtcgcatgtt gggtcgagac
     5341 gaacaggttg acgggcttgc caggtccgcc aaccgggcgc acgtagatct cgcccttgtc
     5401 gacgtcgcac tcaaggacga tctctccggc cgggttgatc gagccggtcg gtggcatcgg
     5461 tgccgcgccc ggctgggcgg gtgccgcgtt ggtgatcgcc gagccgcagt tggtcgagta
     5521 aatgttgccg aagacgttcg tgatggacga gccgtcgacg cggatacggg taggctcttt
     5581 gatcgacagg accgcctcga cggagacgcc gtcgctggct tcgacgagct gaagcgcgtg
     5641 cgagggcgtc gcgctaacga gcaggagtac cgttggaatc agcagcgccg acgcgaactt
     5701 gctggccagg ttgcgcatac gggatctcct tg
//
LOCUS       DAOORV010000205         5707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17071_length_5707_cov_6.446568, whole genome shotgun sequence.
ACCESSION   DAOORV010000205 DAOORV010000000
VERSION     DAOORV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17071_length_5707_cov_6.446568"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKV56_17080"
     CDS             <1..485
                     /locus_tag="PKV56_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69569.1"
                     /translation="CIIVSMYGGGFAAMPAYLADLFGTQMVGAIHDRLLTAWATAGIL
                     GPVIVNYMREYQIGLGLPREQVYNQTMYILVGMLAIGFVCNLLVRPVADKWFMTPDEL
                     AEEKRLAHDRAMTPDDAAAVLAAPRSNPAWMVLAWAAVSLPLAWGVFRTVSSARQFFH
                     "
     gene            563..2473
                     /locus_tag="PKV56_17085"
     CDS             563..2473
                     /locus_tag="PKV56_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOX69570.1"
                     /translation="MQTVRQWPLGAKLALVAAPFLLVAICSIAVLVWMSLQLEGGAAS
                     VNEAGRMRMQAYRMMLSVGTGDLQALPKQRAEFEASLQLLRQGDPERPLFVPWDDTVQ
                     LRFAEVERDWVRYRDRFAGALPPSASNSLASDAAAFASHIDAFVSAIEAHLSRWTSLM
                     HLLQIGLMAFAVIGAAVLLYTGYLFVLEPVGQLKQAAHRIQSGDLGARVSCATTDEFG
                     TLAAGFNDMAEQLQSMYRSLESRVREKTAQLEEKHERLESLYEVTNLVTRALTLDELA
                     SGFTKSVARIAHADGVALRWSDESNRRYLMLASHGLPQAMLEAEHCLMAGDCHCGAVD
                     APPGARVIPIRAMEPARMGHCARAGFETVVSVPIRLHDRLMGEVDLFFHAQVDLSEAE
                     RSLLDALSVQLAGAMENLRLSALGLEAAVSQERRFLASELHDSIAQSLAFLKIQVQLM
                     RDAIASGDPEQVQHVLGEIDVGVRESYGNVRELLMHFRTRANAEDIEPALMTTLRKFE
                     HQTGLKATLHMQGQGMPLAPDTQIQVLHIVQEALSNVRKHARAGQVWLDVQQQPQWRL
                     EVRDDGLGFSSEQGPPDETHVGLRIMAERADRLGARFEVLSTPGRGTSIVLTLPASQK
                     APLDNSLQAQAA"
     gene            2479..3159
                     /locus_tag="PKV56_17090"
     CDS             2479..3159
                     /locus_tag="PKV56_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX69571.1"
                     /translation="MSSMAPRIRIFVIDDHTLFRRGLTALLQRDPQFEVVGDAADAGE
                     AQRRAAALQPDVILLDNHLPGVNGVDALPALLEVVPGVRVLMLTVSEDERDLSAALRG
                     GACGYLLKTIEGDALSAAIRRAMRGECVIADEMTGKLVAAYRDAASPPSAAPVTVLPA
                     SRLDALSPRELEILRGIAQGQSNKEIARALGIAETTVKIHVQHILRKLDVSSRVHAAV
                     IASEHGLG"
     gene            3324..4598
                     /locus_tag="PKV56_17095"
     CDS             3324..4598
                     /locus_tag="PKV56_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HOX69572.1"
                     /translation="MSKSKNSQALAVLLVSTLAFTVCFMVWMMFGVIGIPIKKTLGLN
                     ATEFGLLTATPVLTGSLIRVPLGIWTDKYGGRIVMTLLMAATVPAIWLMSYATAYWHF
                     LVIGLFVGLAGGSFSVGTPYVARWFPKNRQGFAMGVYGAGNSGAAVNKFVAPALLVAF
                     GWTMVPMVYAAIMLGTVVLFWMFSHSDPAHLVPSNTKFTDQLKMLKEPKVLKYCQYYS
                     IVFGGYVALSLWMVQYYVGEFGLDIRSAALLAACFSLPGGVLRAVGGWLSDKYGAHSV
                     TWWVMWVSWVCLFLLSYPQTDFTVLTVDGPKTFHLGLNVYTFTGLMFLLGIAFAFGKA
                     SVFKYISDDYPSNIGTVSGIVGLAGGLGGFVLPIMFGALMDLTGIRSSAFMLMYGVVW
                     VSLIWMYWTEVRRAEVMGTNAMEGPFSRSFSR"
     gene            4629..>5707
                     /locus_tag="PKV56_17100"
     CDS             4629..>5707
                     /locus_tag="PKV56_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69573.1"
                     /translation="MNTTLKAGPAVPAPSSGADVTDWRPEDETFWESTGKKIAYRNLW
                     ISVPALLCGFAVWGMWGIITVQMLNLGFPFTPAELFTLTAIAGISGATMRIPASFLVR
                     LAGGRNTIFLTTTMLLAPAIGTGMALQHKDWPLWAFQLMALWSGVGGGNFASSMSNIS
                     TFFPKRLQGTALGLNAGLGNFGVTTMQIVIPLVMTVGMFGAFGGEPMTLVKDSGWIFG
                     KIAAGTPTWIQNAGFAWVLSLVPLSILAWFGMNNLKTVSPDTGIPVVAFAKVTYLYTL
                     AFIPAVFMLYLYLPAPTGLGILNMWVAIPLDIGLALLVMRVAAFGAMKENVAKQFAIF
                     GNKHTWSMTALYIVTFGSFIGFSMAL"
BASE COUNT          882 a         1835 c         1908 g         1082 t
ORIGIN      
        1 tctgcatcat cgtcagcatg tatggcgggg gctttgccgc catgccggcc tacctggccg
       61 acctgttcgg cacgcagatg gtgggcgcga tccatgaccg gctgctgacc gcctgggcca
      121 cggccggcat cctcggcccg gtgatcgtca actacatgcg cgagtaccag atcgggctgg
      181 ggctgccgcg cgagcaggtc tacaaccaga ccatgtacat cctggtgggc atgctcgcga
      241 tcggcttcgt ctgcaacctg ctggtgcgac cggtggccga caagtggttc atgacgcccg
      301 atgaactggc cgaggagaag cgtctggcgc atgaccgggc catgacgccc gacgatgcag
      361 cggcggtcct cgcggcaccg cgctccaacc cggcctggat ggtgctggcg tgggcggccg
      421 tgtcgctgcc tctggcctgg ggcgtcttcc gaacggtgtc cagcgcccgg cagttctttc
      481 actagcgccc gcgttgaacg atgatccgcg ggtctgcaag cggcagtccc catcctgaac
      541 ggcgagaccc ccagttcatt caatgcaaac cgttcgtcag tggcccctcg gggccaaact
      601 tgccctggtg gctgctccgt tcctgctggt ggcgatctgc tctattgccg ttctggtgtg
      661 gatgtcgctg cagctcgagg gcggggccgc ttcggtcaac gaggcgggcc ggatgcgcat
      721 gcaggcctac cgcatgatgt tgtcggtcgg caccggtgat ctccaggcgc tgccgaagca
      781 gcgggccgag ttcgaggcca gcctccagct gctgcgccag ggcgaccccg aacggccgct
      841 tttcgtgccc tgggacgaca ccgttcagct gcgtttcgcc gaggtcgagc gcgattgggt
      901 gcgctaccgc gatcgctttg ccggtgcgtt gccgccctcg gcgtcgaaca gcctggcctc
      961 cgacgcggcg gctttcgcct cacacatcga tgcgttcgtt tccgccatcg aggcgcatct
     1021 ctcgcgctgg acgtcgctga tgcacctgct gcagatcggc ctgatggcgt ttgcggtcat
     1081 cggcgctgcc gtgcttctct acaccggcta tctgttcgtg ctggaaccgg tcggccagct
     1141 gaagcaggcg gcccatcgca tccagagcgg cgacctgggc gctcgcgtgt cctgtgccac
     1201 gaccgacgaa ttcggcacgc tggccgcagg cttcaacgac atggccgagc agttgcagtc
     1261 gatgtatcgc agcctggaga gccgcgttcg cgagaagacc gcgcagctcg aggaaaagca
     1321 cgagcggctg gaaagcctct acgaagtcac caacctcgtc acgcgggcgc tgacgctgga
     1381 cgaactggcc tccggcttca cgaagagcgt ggcgcgcatc gcgcacgcgg acggggtggc
     1441 gctgcgctgg tccgacgaga gcaatcgccg ctacctgatg ctggcttcgc atggcctgcc
     1501 gcaggcgatg ctggaggccg agcactgcct gatggccggc gattgccatt gcggggcggt
     1561 cgatgcgccg cccggagccc gcgtgattcc gatccgggcg atggagcctg cacgcatggg
     1621 acattgcgcc cgggcgggat tcgagaccgt cgtgtcggtc ccgatccggc tgcacgaccg
     1681 gctgatgggt gaggtcgatc tctttttcca cgcccaggtc gatctttcgg aggctgaacg
     1741 ctcgttgctc gacgcgctgt cggtacagct tgccggggcg atggagaacc tgaggctcag
     1801 cgcgctcgga ctcgaggcgg cggtgtcgca ggagcgccgc ttcctggcaa gcgaactgca
     1861 cgactcgatc gcgcagtcgc tggccttcct gaagatccag gtgcagctga tgcgcgatgc
     1921 catcgccagc ggcgatccgg aacaggtcca gcacgtgctc ggcgagatcg atgtcggggt
     1981 gcgcgaaagc tacggcaatg tgcgtgagct gctgatgcat ttccgcaccc gtgccaatgc
     2041 ggaggacatc gagcctgcgc tgatgaccac gctgcgcaag ttcgagcacc agacgggact
     2101 gaaggcgacg ctgcacatgc aggggcaggg catgccgctg gcgccggaca cgcagatcca
     2161 ggtgctgcac atcgtgcagg aggcgttgtc caatgtccgc aagcatgcgc gggccggcca
     2221 ggtgtggctg gacgtgcagc agcagccgca atggcggctg gaggtgcgcg acgatggtct
     2281 cgggttctct agcgagcagg gcccgccgga tgaaacccac gtcgggcttc gcatcatggc
     2341 ggagcgtgcc gatcgcctgg gggctcgttt cgaggtgctg tcgacgccgg gtcggggcac
     2401 gtcgatcgtg ctgacgctgc ccgccagcca gaaggcaccg ctcgacaact cactgcaggc
     2461 gcaggccgcc tgaaacgcat gagcagcatg gccccccgga tccgcatctt cgtcatcgat
     2521 gaccacacgc tgttcaggcg cggcctgacg gccctgctgc agcgcgaccc gcagttcgaa
     2581 gtggtcggcg acgcggccga tgcgggcgag gcgcagcgcc gggccgcggc gctgcagccc
     2641 gatgtgatcc tgctcgacaa ccacctgccc ggggtcaacg gcgtcgatgc gctgccggcg
     2701 ctgctcgagg tggttccggg tgtgcgcgtg ctgatgctga ccgtcagcga ggacgagcgc
     2761 gatctttccg ccgccttgcg cggcggggcc tgcggctacc tgctcaagac catcgagggc
     2821 gatgcgttgt cggcggcgat ccggcgggcg atgcgcggcg agtgcgtgat cgccgacgag
     2881 atgacgggca agctggtggc cgcctaccgc gatgcggcca gcccgccgtc cgcagctccg
     2941 gtgacggtct tgccggcgtc caggctcgat gcgctgtcgc cgcgcgagct ggagatcctg
     3001 cggggcatcg cccaggggca gagcaacaag gaaatcgcgc gcgcgctcgg cattgccgag
     3061 acgaccgtga agatccatgt gcagcacatc ctgcgcaagc tcgacgtgag ctcgcgcgtg
     3121 catgccgccg tgatcgcatc cgagcacggc ctcggttgat ctggatcaat cgaccgccac
     3181 tttcctgatc gggtagttct tcggaactat ggcggccacc ggccgcatcg ttcttctgca
     3241 gtgcctcgga cctcccagcg acggatgccg cgatgacgcg ctgatttcta cagtctgctc
     3301 atgcctaacg agtggactgc aagatgagca agagcaagaa cagccaggcg ctggcggtgc
     3361 tgctggtgag cacgctggcc ttcaccgtgt gcttcatggt gtggatgatg ttcggcgtga
     3421 tcggcatccc gatcaagaag acgctgggcc tcaacgcgac cgaattcggc ctgctgacgg
     3481 cgacaccggt gctcacaggc tcgctgatcc gcgtgccgct cggcatctgg actgacaagt
     3541 acggcggtcg catcgtcatg acgctgctga tggcggccac cgtgcccgcc atctggctga
     3601 tgagctacgc gaccgcctac tggcatttcc tggtcatcgg actgttcgtc gggctcgcgg
     3661 gtggttcgtt ctcggtcggt acgccgtacg tcgcccgctg gtttccgaag aaccgccagg
     3721 gcttcgcgat gggcgtctac ggggcaggca actcgggcgc ggcggtcaac aagttcgtgg
     3781 cgcccgcgct gctggtggcc ttcggctgga cgatggtccc gatggtctac gccgcgatca
     3841 tgctcggcac ggtggtgctg ttctggatgt tcagccacag cgatccggcg cacctggttc
     3901 cgtcgaacac gaagttcacc gaccagctca agatgctgaa ggagccgaag gtgctgaagt
     3961 actgccagta ctacagcatc gtgttcggcg gctacgtggc gctgtcgctg tggatggtgc
     4021 agtactacgt cggcgagttc ggcctcgaca tccgctccgc cgcgctgctc gctgcgtgct
     4081 tctcgctgcc cggtggcgtg ttgcgtgccg tcggcggctg gctgtccgac aagtacggtg
     4141 cgcacagcgt cacctggtgg gtgatgtggg tcagctgggt ctgcctgttc ctgctgtcgt
     4201 acccgcagac cgatttcacg gtgctgaccg tcgacggtcc gaagaccttc cacctcgggc
     4261 tcaacgtcta cacattcacc ggcctgatgt tcctgctggg cattgcgttc gccttcggca
     4321 aggccagtgt cttcaagtac atcagcgacg actatccgtc caacatcggg accgtcagcg
     4381 gcatcgtggg cctggccggt ggactcggcg gcttcgttct gccgatcatg ttcggagcgc
     4441 tgatggacct gaccggtatc cgctccagcg ccttcatgct gatgtacggc gtggtctggg
     4501 tctcgctgat ctggatgtac tggaccgagg tgcgccgcgc cgaggtgatg ggaaccaacg
     4561 ccatggaagg accgttctcg cggtcgttct cccgctgatc cgtgcgctga atttttgaaa
     4621 gagactccat gaacaccaca ctcaaggccg gccccgccgt gcctgcaccc tccagcggtg
     4681 ccgacgtcac ggactggcgt cccgaagacg agaccttctg ggaaagcacc ggcaagaaga
     4741 tcgcctaccg caacctgtgg atctcggtgc cggccttgct gtgcggcttc gcggtctggg
     4801 gcatgtgggg aatcatcacc gtccagatgc tcaacctggg cttcccgttc acaccggccg
     4861 agttgttcac gctgacggcg attgccggga tatcgggcgc cacgatgcgc attccggcgt
     4921 cgttcctggt tcgcctggcc ggtggccgca acaccatctt tctcaccacc acgatgctgc
     4981 tggcaccagc catcggcacc ggcatggcgt tgcagcacaa ggactggccg ctgtgggcgt
     5041 tccagctgat ggcgctgtgg tcgggcgtgg gcggcggcaa tttcgccagc tcgatgtcga
     5101 acatcagcac cttcttcccg aagcggttgc agggcaccgc gctcggcctg aatgcgggcc
     5161 tgggcaactt cggcgtgacc accatgcaga tcgtcatccc gctggtgatg acggtgggca
     5221 tgttcggcgc gttcggcggt gaaccgatga cgctggtgaa ggacagcggc tggatcttcg
     5281 gaaagatcgc cgccggcacg ccgacctgga tccagaacgc cggcttcgca tgggtgttgt
     5341 cgctcgtgcc gctgtccatc ctggcctggt tcgggatgaa caacctcaag acggtctcgc
     5401 ctgacaccgg catccccgtc gtcgcgttcg ccaaggtgac ctatctctac accctggcct
     5461 tcatccctgc cgtcttcatg ctctacctgt acctgccggc acccaccggt ctgggcatcc
     5521 tgaacatgtg ggtggccatt ccgctggaca tcggcctggc tttgctggtg atgcgagtcg
     5581 ccgcgttcgg tgcgatgaag gaaaacgtcg ccaagcagtt cgccatcttc ggcaacaagc
     5641 acacctggtc gatgacggcg ctgtacatcg tcaccttcgg ctcgttcatc ggcttctcga
     5701 tggcgct
//
LOCUS       DAOORV010000206         5696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17117_length_5696_cov_11.552562, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000206 DAOORV010000000
VERSION     DAOORV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17117_length_5696_cov_11.552562"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKV56_17105"
     CDS             complement(<1..563)
                     /locus_tag="PKV56_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOX69574.1"
                     /translation="MLIGDRTVGADARPYVIAEMSGNHNQSLDRALRIVEAAAASGAD
                     AIKLQTYTPDTMTLDISAPGFLIDDEKSLWAGRQLYDLYREAHTPWAWHRPIMERAAA
                     LGLHCFSTPFDDTAVDFLETLDVPAYKIASFECTDLPLIRKVAATGKPMIISTGMATL
                     AEIDETVRTARAAGCRDLVLLKCTSTYP"
     gene            complement(740..1375)
                     /locus_tag="PKV56_17110"
     CDS             complement(740..1375)
                     /locus_tag="PKV56_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOX69575.1"
                     /translation="MTAVSILCTDPKHAVNAWLERWAAAVAPRVEVRICRDWRELTGG
                     DLLFLVSCHQIISASVRALFRHTLVLHASALPAGRGMSPHIWQILEGCEQLTMTLLEA
                     EDALDTGRIWHQLDVPIPVTAVCDEINARIFEAEIALMDWALAHRETVVPREQSGEPS
                     MYRKRVPADSRIDPLRPLADAFDLLRVADPERYPAFFEHRGRTYRLRIDPL"
     gene            complement(1375..2442)
                     /gene="pseG"
                     /locus_tag="PKV56_17115"
     CDS             complement(1375..2442)
                     /gene="pseG"
                     /locus_tag="PKV56_17115"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HOX69576.1"
                     /translation="MKIAIRADASASIGLGHIKRCLSLAQALRDEDAEVLFFVRATDV
                     DAAGEIARRGFDCHAIAEGPVRDHDTEHDDARAFIASSRSFEPDAVLADHYALGADWH
                     RAVHQGTGAQMAAIDDLGDRPMAVDLLVDPNFAADHTAKHARHGDAPPVLLGGPGYAL
                     LAPAYADAPRGEPRDSVASIGIFMGGTDEANLSALALTVVRDDLGFRGAVEIATTSGN
                     PHLAELRARVEADAGTQLTIDQPDLAAFYARHDLHLGAGGGATWERCCLGAPTLALLA
                     APNQREVLLPLQNMGVMRVVTAEPPSASDIVPPLRELIVDAALRHSLSVHGRALVDGL
                     GARRVARRLLDLQRPRPRARQ"
     gene            complement(2439..3146)
                     /gene="pseF"
                     /locus_tag="PKV56_17120"
     CDS             complement(2439..3146)
                     /gene="pseF"
                     /locus_tag="PKV56_17120"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HOX69577.1"
                     /translation="MTRLAVIPARGGSKRIPRKNIRAFAGRPMIGYAIDLARNSGLFD
                     HVVVSTDDAEVADVARSLGAEVPFMRPDELANDHTATVPVVAHAIRACRELGWRVDTV
                     CCLYPAVPLLRPSDLSDALAMLEQGDAPYVFPVLAYRSPIQRALRRDADGRSAPLHPE
                     HAQTRTQDLPKAYHDAGQFYWGRASSWLDGLALHADARTLVLDEGSAVDIDTPADWAL
                     AEALYAQRGARLEAVTP"
     gene            complement(3149..3793)
                     /locus_tag="PKV56_17125"
     CDS             complement(3149..3793)
                     /locus_tag="PKV56_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69578.1"
                     /translation="MSFNTPQEKFWAYDHAHDYIARNRSFDHDLGARAWATMLAKAER
                     PVRNFLECGCNIGRNITQLAQALPEAQPSIIEISDPAFQFVTAQHEFGHAFNGAILDA
                     DLPAQGFDLVFTLGVLIHIHPDQLLAHMARMHAWSSRYVLMGEYFSRTPQMIEYRGES
                     DRLFKSDFGKTFAENFDVDVVDCGFLWGHQYDAAGFDDITWWLFEKRQSAVGHA"
     gene            complement(3820..4974)
                     /gene="pseC"
                     /locus_tag="PKV56_17130"
     CDS             complement(3820..4974)
                     /gene="pseC"
                     /locus_tag="PKV56_17130"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOX69579.1"
                     /translation="MIPYSRQDIDESDIAAVTAVLRSDFVTQGPVVAQFEAAFAARHQ
                     TAHAVAVSNATSALHLACLALGVGPGSRVWTSPNSFLASANCALYCGASVDFVDIDPA
                     TRNLSVASLERKLRTASAAGLLPQVVIPVDFAGLPADLREMRALADRYGFRLLEDASH
                     ATGASYQGQPVGSRHADATVFSFHAVKVLTTAEGGMVTTNSAALAQRVRTLRSHGVTR
                     DAAEMEQPPEGAWCYEQQALGFNHRMTDVQAALGLSQLARLDTMHARRCALADRYDTL
                     LVDLPVWLPPRAPDRVSAWHLYAIEIDAERCNRSRAEVFEAMRSAEIGVNVHYIPIHT
                     QPYYARLGFKRGDFPAAERYYARTLSLPLFPSMTPAEQDRVVSTLRQALA"
     gene            complement(4993..>5696)
                     /locus_tag="PKV56_17135"
     CDS             complement(4993..>5696)
                     /locus_tag="PKV56_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOX69580.1"
                     /translation="CLDQQVRRVVALSTDKAAAPINLYGATKLCSDKLFVAANNIKGN
                     RDLRFSVVRYGNVMGSRGSVIPFFLAQRPSGVLPITDPQMTRFNISLQEGVEMVLWSI
                     ERAWGGEILVPKIPSYRITDVATAIAPECTQKIVGIRPGEKIHEEMITASDSLNTADL
                     GDYFAILPTSGEYTVDDYVARHHARLVAPGFAYDSGSNPHFLSVSQLRELIAAHVDGA
                     KTVERRRRVSAVAHA"
BASE COUNT          878 a         1867 c         2045 g          906 t
ORIGIN      
        1 gggtaggtgc tggtgcactt gagcagcacc aggtcgcggc agccggcggc acgcgcggtg
       61 cgcacggtct cgtcgatctc ggccagcgtg gccatgccgg tggagatgat catcggcttg
      121 ccggtggccg ccaccttgcg gatcagcggc aggtcggtgc actcgaacga ggcgatcttg
      181 taggctggca catccagcgt ctcgaggaag tcgaccgcgg tgtcgtcgaa cggcgtgctg
      241 aagcagtgca ggcccagcgc cgcggcgcgc tccatgatcg ggcggtgcca ggcccacggc
      301 gtgtgcgcct cgcggtacag gtcgtagagc tggcggcccg cccacaggct cttctcgtcg
      361 tcgatcagga agcccggcgc actgatgtcc agcgtcatcg tgtcgggcgt gtaggtctgc
      421 agcttgatcg cgtcggcgcc cgaagcggct gcggcctcga cgatgcgcag cgcgcggtcg
      481 agcgactggt tgtggttgcc gctcatctcg gcgatcacgt aaggccgcgc gtcggcgccg
      541 acggtgcggt cgccgatcag catcggtcgc ctcgcggtac gtgcgcgagg cccggtcggg
      601 gcggtgcggt atcgaacatg gccgaaggat cctcgtggag cggcccatcg gcgggcagca
      661 tcctgtcagc cgatcttagg aaggccggcc ggatcgatgc ggtggataag cggccggttc
      721 gaccgccagt tcgcgtgggc tacagcgggt cgatgcgcag ccggtaggtg cgtccgcggt
      781 gctcgaagaa cgccgggtag cgctcagggt cggccacgcg cagcaggtcg aacgcgtcgg
      841 ccagcggacg cagcgggtcg atgcgactgt cggccggcac ccgcttgcgg tacatgctgg
      901 gctcgcccga ctgctcgcgc gggacgaccg tctcgcggtg tgcgagggcc cagtccatca
      961 gcgcgatctc ggcctcgaag atccgcgcgt tgatctcgtc gcacaccgcg gtgacgggaa
     1021 tcggcacgtc gagctggtgc cagatgcgac cggtgtcgag cgcgtcctcg gcctccagca
     1081 gcgtcatcgt cagttgctcg cagccctcga ggatctgcca gatgtgtggc gacatgccgc
     1141 gacccgccgg cagcgcgctg gcgtgcagca ccagcgtgtg cctgaaaagt gctcgcaccg
     1201 aggcggagat gatctggtgg caagacacca ggaagagcaa gtcgccgccc gtcagttctc
     1261 gccagtcgcg gcagatgcgc acctcgaccc ggggcgccac ggccgcggcc cagcgctcga
     1321 gccacgcgtt gaccgcgtgc ttcgggtcgg tgcacaggat ggaaacggcc gtcatcactg
     1381 tcgcgcgcgc ggtcgcgggc gctgcaggtc gagcagccgg cgggccaccc ggcgcgcacc
     1441 cagtccgtcg acgagcgcgc ggccgtgcac cgacagcgaa tggcgcagtg ccgcgtcgac
     1501 gatcagttcg cgcagtggcg gcacgatgtc ggaagcgctc ggcggttcgg cggtgacgac
     1561 gcgcatcacg cccatgttct gcaggggcag cagcacttcg cgctggttcg gggcggcgag
     1621 cagcgccagc gtgggcgccc cgaggcagca gcgctcccag gtcgcgccgc cacccgcacc
     1681 gaggtgcagg tcgtgccgtg cgtagaacgc cgcgagatcg ggctggtcga tggtgagctg
     1741 cgtgccggca tcggcctcga cccgtgcgcg cagttcggcc agatgcggat tgcccgaggt
     1801 ggtcgcgatc tcgaccgcac cgcgaaaacc caggtcgtcg cgcacgaccg tcaacgccag
     1861 tgcgctcagg ttggcttcgt cggtgccccc catgaagatg ccgatgctcg ccacgctgtc
     1921 gcgcggttcg ccacgcggcg catcggcgta ggcgggagcc agcagtgcat agccggggcc
     1981 gcccagcagc accggcggtg catccccgtg acgcgcgtgc ttggcggtgt ggtcggcagc
     2041 gaagttggga tcgacgagca gatcgaccgc catcggacgg tcgccgaggt cgtcgatcgc
     2101 ggccatctga gcgccggtgc cctggtgcac ggcccggtgc cagtcagcgc cgagcgcgta
     2161 gtgatcggcc agcacggcgt cgggctcgaa gctgcggctg ctggcgatga aggcgcgcgc
     2221 gtcgtcgtgc tcggtgtcgt gatcacgcac cggcccctcg gcaatggcat ggcagtcgaa
     2281 gccacgccgt gcgatctcgc ccgccgcatc gacgtcggtc gctcggacga agaacagcac
     2341 ctcggcatcc tcgtcgcgca gggcctgcgc cagcgacagg cagcgcttga tgtgtccgag
     2401 gccgatggaa gccgacgcgt cggcacggat cgcgatcttc atggcgtgac ggcctccagg
     2461 cgagccccgc gctgcgcgta cagcgcttcg gcgagcgccc agtcggcagg cgtgtcgatg
     2521 tcgaccgcac tgccctcgtc gagcaccagc gtgcgggcat cggcatgcag tgccagcccg
     2581 tcgagccagg acgacgcacg gccccagtag aactggccgg catcgtgata cgccttcggc
     2641 aggtcctgcg ttcgcgtctg cgcgtgttcg ggatgaagcg gcgcgctgcg gccgtcggca
     2701 tcgcgccgca gcgcgcgctg gatcggcgat cggtacgcca gcaccgggaa gacatagggc
     2761 gcatcgccct gctccaacat ggccagcgca tcgctcaggt cacttgggcg cagcagcggc
     2821 accgccgggt agagacagca cacggtgtcg acgcgccagc ccagctcgcg gcaggcacgg
     2881 atcgcgtgcg cgaccacagg cactgttgct gtgtggtcgt tcgccagttc gtcgggtcgc
     2941 atgaacggca cttcggcacc gaggctccgg gccacgtcgg cgacttccgc gtcgtcggtg
     3001 gacacgacca cgtggtcgaa caggccgctg ttgcgcgcca ggtcgatcgc gtagccgatc
     3061 atcggccgcc ctgcgaacgc acggatgttc ttgcgcggaa tgcgcttgct gccgccgcgc
     3121 gcggggatca ctgccaggcg cgtcatgctc aggcgtggcc gacggcgctc tggcgcttct
     3181 cgaacagcca ccaggtgatg tcgtcgaagc cggcagcgtc atactggtgg ccccacagga
     3241 agccgcaatc gaccacgtcg acatcgaagt tctcggcgaa tgtcttgccg aaatcgctct
     3301 tgaaaagccg gtcgctctct ccgcggtact cgatcatttg cggcgttcgg ctgaagtact
     3361 cgcccatcag cacgtaacgc gacgaccagg cgtgcatgcg agccatgtga gccagcagct
     3421 ggtcgggatg gatgtggatc agcacgccga gcgtgaatac caggtcgaag ccctgtgccg
     3481 gcaggtcggc gtcgagaatt gcgccgttga acgcatgtcc gaactcgtgc tgggcagtga
     3541 cgaactggaa ggccgggtcg ctgatctcga tgatcgaggg ctgagcctcg ggcagcgcct
     3601 gggccagctg cgtgatgttg cggccgatgt tgcaaccgca ttcgagaaag ttgcgcaccg
     3661 gccgctcggc cttggcaagc atcgtggccc aggccctggc acccaggtca tggtcgaagc
     3721 ttcggttgcg ggcgatgtag tcatgtgcgt ggtcgtacgc ccagaatttt tcctgcgggg
     3781 tgttgaaaga cattcggtgc tcctggaatt gaaactggat caggccagcg cctgccgcag
     3841 ggtgctgacc actcgatcct gctcggctgg cgtcatcgac gggaacagcg gcagcgacag
     3901 cgtgcgggcg tagtagcgct cggcggcggg gaaatcgccg cgcttgaatc ccagacgcgc
     3961 gtaatacggc tgcgtgtgga tcgggatgta gtgcacgttg acgccgatct ccgcggagcg
     4021 catcgcttcg aacacctcgg cccgcgagcg attgcagcgc tcggcatcga tctcgatcgc
     4081 gtacaggtgc cacgccgaga cccgatcggg cgcacgcggc ggcagccaca cgggaagatc
     4141 gaccagcaag gtgtcgtagc gatcggccag cgcgcagcgg cgcgcgtgca tcgtgtcgag
     4201 ccgcgcgagc tgcgacaagc ccagcgccgc ctgcacatcg gtcatgcggt ggttgaatcc
     4261 cagcgcttgc tgctcgtagc accacgcgcc ctcgggcggc tgctccatct cggccgcgtc
     4321 gcgcgtgacg ccgtgcgagc gcagcgttcg cacccgctgc gccagcgcgg cgctgttcgt
     4381 cgtgaccatg ccgccttcgg ccgtggtgag caccttgacc gcgtgaaagc tgaacacggt
     4441 cgcgtcggca tgacggctgc cgaccggctg cccctggtag ctggcgccgg tggcatggga
     4501 ggcatcctcg agcagccgga agccgtatcg atcggcgagt gcgcgcatct cgcgcaggtc
     4561 ggcgggcaag ccggcgaaat cgaccggaat caccacctgc ggaagcagcc cggcggcgga
     4621 cgcggttcgc agcttgcgct cgagcgaggc caccgacagg ttgcgggtgg ccggatcgat
     4681 gtcgacgaaa tcgacgctcg cgccgcagta aagcgcacag ttggccgagg cgaggaagct
     4741 gttgggactg gtccatacgc gcgaccccgg gccgacgccg agcgccaggc aggccaggtg
     4801 cagcgccgag gtggcgttgc tgacggcgac cgcgtgcgcg gtctggtgtc gtgcggcgaa
     4861 cgcggcttcg aactgcgcga ccaccgggcc ctgcgtgacg aagtcggatc gcagcaccgc
     4921 cgtgacggcg gcgatgtcgc tctcgtcgat gtcctgccgg ctgtacggaa tcaccgtcgt
     4981 ctcgctggcc ggtcaggcgt gcgccacggc cgagacgcgc cggcggcgct cgaccgtctt
     5041 cgcaccgtcg acatgcgcgg cgatcagttc gcgcagttgc gacacgctga ggaaatgcgg
     5101 gttgctgccg ctgtcatagg cgaagcccgg cgcgaccagg cgcgcgtgat ggcgtgccac
     5161 gtaatcatcg acggtgtatt cgccgctggt cggcaggatc gcgaagtagt cgccgaggtc
     5221 ggcggtgttc aggctgtcgc tcgcggtgat catttcctcg tggatcttct cacccgggcg
     5281 gatgccgacg atcttctgcg tgcattccgg cgcgatcgcc gtggccacgt cggtgatgcg
     5341 gtaggacggg atcttcggca ccaggatctc gccgccccag gcacgctcga tcgaccacag
     5401 caccatctcg acgccttcct gcagcgagat gttgaagcgc gtcatctgcg ggtcggtgat
     5461 cggcagcacg ccgctcggcc gctgggcaag aaagaacggg atcaccgaac cgcggctgcc
     5521 catcacgttg ccgtagcgga ccacgctgaa gcgcagatcg cggtttccct tgatgttgtt
     5581 ggcggcgacg aacagcttgt cggagcacag cttggtcgcg ccgtacaggt tgatcggcgc
     5641 agcggccttg tcggtcgaca gcgccaccac gcggcgcacc tgctggtcga ggcagg
//
LOCUS       DAOORV010000207         5694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17130_length_5694_cov_6.489803, whole genome shotgun sequence.
ACCESSION   DAOORV010000207 DAOORV010000000
VERSION     DAOORV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17130_length_5694_cov_6.489803"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /gene="guaA"
                     /locus_tag="PKV56_17140"
     CDS             complement(<1..1459)
                     /gene="guaA"
                     /locus_tag="PKV56_17140"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOX69581.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREAHVLSEVHPCDVTDAWIRDY
                     AKDGSLKGVILSGSHASVYEDTTDKAPQAVFELGVPVLGICYGMQTMAHQLGGKVESG
                     HQREFGFAAVRARGHTALLDGIADFTTPDGAGMLNVWMSHGDKVTELPPGFKLMASTD
                     SCPIAGMADEARRFYAVQFHPEVTHTQQGQAILDRFVRGICAAKPDWVMRDHIAEAVQ
                     KIRAQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGDLVMDMF
                     VGKLHAKVIRVDASELFLGKLAGVSDPEAKRKIIGGEFVTVFKQEAAKLTSAGAKGAK
                     WLAQGTIYPDVIESGGAKSKKAVTIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVRELG
                     VALGLPPGMVYRHPFPGPGLGVRILGEVKKEYADLLRRADDIFIQELHNFRDEATGKS
                     WYDLTSQAFTVFLPVKSVGVMGDGRTYDYVVALRAV"
     gene            complement(1499..2725)
                     /locus_tag="PKV56_17145"
     CDS             complement(1499..2725)
                     /locus_tag="PKV56_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69582.1"
                     /translation="MPFILIAVLIDMVSIGIIVPVLPALVGSLTGSQADHAFWLGVVS
                     FAFGIANFFGAPILGALSDRYGRRPVLLLGFCGLAFTFFATALSTTMWMLIVARLLGG
                     GLQANAAVANAYVADISAPEERAKRFGMLGAMFGIGFILGPAMGGVLGAIDLHLPFFV
                     AGTFALVNLLYGCFVLPESLPADRRRAFAWRSANPITSLKALAKLKGVGRLVGVIACT
                     GLAQGVLYNSWVLYATFKFGWDSSANGWSLAAIGVVSVFVQGYLLGKLLKRFPPPRLA
                     VLGLISSMLAYALWGAAVAGWMMYAVIFANVLGFTVNSSIQSIVSGAADERSQGQTLG
                     AVSALNSLTAVMGPLITTPLLVMVSDLPQGHWAIGSPLYCGALLQATALYLAWTHFRR
                     MGSRAPQAVAAASGTS"
     gene            complement(2809..4278)
                     /gene="guaB"
                     /locus_tag="PKV56_17150"
     CDS             complement(2809..4278)
                     /gene="guaB"
                     /locus_tag="PKV56_17150"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOX69583.1"
                     /translation="MRLLGKALTFDDVLLVPAFSQVLPRDTSLATRISRNIQLNLPLV
                     SAAMDTVTEARLAIAIAQEGGMGIVHKNLSPRQQAAEVARVKRYESGVLRDPITITPE
                     TAVRQVVELSRQHGVSGFPVLEGNRVVGIVTNRDLRFETRLDAPVREIMTPRERLITV
                     DERATLEDGKALMHLHKLERVLVVNAAGELRGLMTVKDITKQTSFPNAARDAQGKLRV
                     GAAVGVGEGTEERVEALVRAGVDAIVVDTAHGHSAGVIERVRWVKQHYPQIDVIGGNI
                     ATGAAALALVEAGADGVKVGIGPGSICTTRIVAGVGVPQITAIDNVAKALAGSGVPLI
                     ADGGIRYSGDIAKAIAAGAHTVMMGGVFAGTEEAPGEIVLFQGRSYKSYRGMGSIGAM
                     QQGSADRYFQDNTGANPNADKLVPEGIEGRVPYKGSMVSIVFQMAGGIRASMGYCGCA
                     TIEDMRSRAEFVEITSAGIRESHVHDVQITKEAPNYRME"
     gene            4412..4909
                     /locus_tag="PKV56_17155"
     CDS             4412..4909
                     /locus_tag="PKV56_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOX69584.1"
                     /translation="MRHWPAALFGIVLLSSLCLPAAAQWKWRDKNGQTQYSDLPPPQG
                     TPASAVLQRPTGAKLRSVLPADAEAAASAPLAPAPKTVEPELEAKLRKAEQEKAAKSK
                     AEDEKIALQKAENCSRARNQMRVIDEGLRVARINEKGEREVLDDKGRAEEGNRARAII
                     ASDCK"
     gene            complement(4887..5198)
                     /locus_tag="PKV56_17160"
     CDS             complement(4887..5198)
                     /locus_tag="PKV56_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOX69585.1"
                     /translation="METAEAEVLQIEVVHSPAAGEMRRIRLSLPAGSRVADALRLSGL
                     LPQGVDFGMSRLKVGVWGQLKPFDHPLRDHDRVEVYRPLLIDPKDARRRRQRTTCSRK
                     R"
     gene            complement(5185..5628)
                     /locus_tag="PKV56_17165"
     CDS             complement(5185..5628)
                     /locus_tag="PKV56_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOX69586.1"
                     /translation="MKHVKKSVLLWYSPHEMYELVTGVADYPKFLPWCARSEVLEHTD
                     ASMTARLHLAYAGIRHAFTTRNEHDSDSAVRVQLVDGPFSQLDGQWLFHRVGSEAQAC
                     KIEFELRYAFASVALEAVVSPVFDRIANTFVESFVKRAAQVYGDR"
BASE COUNT         1026 a         1880 c         1849 g          939 t
ORIGIN      
        1 gcacggcgcg cagcgccacc acgtagtcgt aggtgcggcc gtcgcccatc acgccaacgc
       61 tcttgacggg aaggaaaacg gtgaaggctt ggctggtcag gtcgtaccag ctcttgcccg
      121 tggcctcatc cctgaagttg tgcagctcct ggatgaagat gtcgtcggcg cgacgcagca
      181 ggtcggcgta ttccttcttc acctcaccga ggatgcgcac acccagaccc ggtcccggga
      241 acggatgccg atagaccatg ccgggaggca gcccgagtgc cacgccgagt tcgcgcacct
      301 cgtccttgaa caggtcgcgc aacggctcca gaagcttgag tcccaactgc tcgggcaggc
      361 cgcccacgtt gtgatgactc ttgatcgtga cggccttctt gctcttggct ccaccggact
      421 cgatcacgtc cgggtagatg gtgccctgcg ccagccattt cgcgcccttg gcgcccgcac
      481 tggtcaactt ggccgcctcc tgcttgaaga cggtgacgaa ctcgccgccg atgatcttgc
      541 gcttggcctc ggggtcgctg acgccggcga gcttgccgag gaacagctcg ctcgcatcga
      601 cgcggatcac cttggcgtgc agcttgccga cgaacatgtc catcacgagg tcgccctcgt
      661 tgaggcgcag caagccgtgg tcgacgaaca cgcaggtgag ctggtcgccg atcgcacggt
      721 ggatcaacgc cgccgccacc gatgagtcga caccgcccga cagtccgaga atgacttcct
      781 catcgccgac ctgcgcacgg atcttctgga ccgcttcggc gatgtggtcg cgcatgaccc
      841 agtcgggctt cgcggcgcag atgccacgca cgaagcgatc gaggatggcc tggccctgct
      901 gcgtgtgggt cacttcgggg tggaactgca cggcatagaa gcggcgcgct tcatcggcca
      961 tgccggcgat cgggcaactg tcggtgctcg ccatcagctt gaagccgggc ggcagttcgg
     1021 tgaccttgtc cccgtggctc atccagacgt tcagcatgcc ggcgccgtcc ggcgtggtga
     1081 agtccgcgat gccatcgagc agtgccgtgt ggccgcgtgc gcgcaccgcc gcgaagccga
     1141 actcccgttg gtggccgctc tcgaccttgc cgccgagttg atgcgccatg gtctgcatgc
     1201 cgtagcagat gccgagcacg ggcacgccga gctcgaacac cgcctgcggc gccttgtccg
     1261 tcgtgtcttc gtagacgctg gcgtggctgc ccgacaggat cacgcccttg agcgagccgt
     1321 ccttcgcgta gtcgcggatc catgcatccg tgacgtcgca cggatgcact tcgctcagca
     1381 catgggcttc gcgcacccgg cgtgcgatca gttgggtgac ctgtgagccg aagtcgagga
     1441 tgaggatctt gtcgtgcatg ggttgcttgt atcgaggggt gtgagacccg gcgcgcggtc
     1501 aggatgtgcc ggaagcagcc gccacggcct gcggcgcacg gcttcccatc cggcggaaat
     1561 gcgtccaggc caggtacagg gccgtggcct gcagcagcgc gccacagtag agcggtgaac
     1621 cgatggccca gtgcccctgc ggaaggtcgg agaccatcac gagcagcgga gtggtgatca
     1681 gcggccccat cacggcagtc aggctgttga gcgcgctcac cgcgccgagc gtctggccct
     1741 ggctgcgctc gtcagcagcg cccgagacga tgctctggat cgacgaattg acagtgaagc
     1801 cgagcacgtt ggcgaagatc accgcgtaca tcatccagcc tgccacggcc gcgccccaca
     1861 gcgcataggc cagcatggac gagatgagac ccaggaccgc cagacgcggc ggcgggaacc
     1921 tcttgagcag cttgccgagc aggtagccct gcacgaacac cgagaccacg ccgatggcgg
     1981 ccagcgacca gccgttggcg ctcgagtccc agccgaactt gaaggtggcg tacagcaccc
     2041 agctgttgta gagaacgccc tgcgcgagcc cggtgcaggc gatcacaccg accaaccggc
     2101 ccacgccctt gagtttcgca agcgccttca aagaggtgat cgggttggct gatcgccatg
     2161 caaacgcacg cctgcggtcc gcgggcagcg attccggaag gacgaaacag ccgtagagca
     2221 gattgaccag ggcaaacgtg ccggccacga agaacggcag gtgcaggtca atggcgccga
     2281 gcacaccgcc catggctggc ccgaggatga agccgatgcc gaacatcgcg ccgagcatgc
     2341 cgaagcgctt ggcccgctcc tccggggccg agatgtcagc cacgtaggcg ttggccaccg
     2401 ctgcgttggc ctgcaggcca ccacccagca gccgcgccac gatcagcatc cacatggtgg
     2461 tggacagcgc ggtggcgaag aacgtgaagg ccaatccgca aaagcccagc agcagcaccg
     2521 gccgccggcc gtagcggtcc gacagcgcgc ccaggatcgg cgccccgaag aaattggcga
     2581 tgccgaaggc gaaactgacc acgccaagcc agaaggcatg gtcggcctgc gagccggtga
     2641 ggcttccgac cagcgccgga agtaccggaa cgatgatgcc gatcgacacc atgtcgatca
     2701 gcacggcgat caatatgaag ggcatcgcgg cccggcgtcg gtcccggtca ggcggcttcg
     2761 acggctcgcc tggttccggg gcgcccgcgt tatcgatcac ggacgtcgtc attccatgcg
     2821 gtaattcggc gcttccttcg tgatctgcac gtcatgcaca tggctctcgc ggatgcctgc
     2881 ggacgtgatc tcgacgaact cggcgcggct gcgcatgtct tcgatcgtgg cgcagccgca
     2941 gtaacccatc gaggcacgga taccgccggc catctggaac acgatgctga ccatcgatcc
     3001 cttgtaggga actcgccctt cgatgccttc gggcaccagc ttgtcggcgt tcggattggc
     3061 gccggtgttg tcctggaagt agcggtcggc gctgccttgc tgcatcgcac cgatcgaacc
     3121 catgccgcgg tagctcttgt agctgcggcc ctggaacagg acgatctcgc cgggcgcctc
     3181 ttcggtgccg gcaaacacgc cacccatcat caccgtgtgt gcgccagcgg caatcgcctt
     3241 ggcgatgtcg cccgagtaac ggatgccgcc gtcggcgatc agcggcacgc cgctgccggc
     3301 cagcgccttg gccacgttgt cgatcgcggt gatctgcggc acgccgacgc ccgcgacgat
     3361 gcgtgtcgtg cagatcgatc ccggtccgat gccgaccttg acgccatcgg cgcccgcctc
     3421 gaccagggcg agtgcggctg cgccggtggc gatgttgccg ccgatcacat cgatctgcgg
     3481 gtagtgctgc ttgacccagc ggacccgctc gatcactccg gcgctgtggc catgcgcggt
     3541 gtcgacgacg atcgcatcaa ccccggcgcg taccagcgcc tcgacccgct cttcggtgcc
     3601 ctcgcccacg ccgaccgccg cgccgacgcg cagcttgccc tgggcatcac gcgcggcgtt
     3661 cggaaagctc gtctgcttgg tgatgtcctt gaccgtcatc agcccacgca gctcgccggc
     3721 tgcattgacg accagcacac gctcgagctt gtgcaggtgc atcagcgcct tgccgtcttc
     3781 cagcgtggca cgctcgtcga ccgtgatgag ccgctcgcgc ggggtcatga tctcgcgcac
     3841 gggcgcatcg aggcgggtct cgaagcgcag gtcgcgattg gtcacgatgc cgaccacgcg
     3901 gttgccttcg agcacaggga agccggagac accgtgttgc cgcgacagtt cgacgacttg
     3961 ccgaacggcg gtttcaggcg tgatcgtgat cgggtcgcgc agcacgccgg attcatagcg
     4021 cttgacgcgg gcaacttcgg ccgcctgctg ccgggggctg aggttcttgt ggacgatgcc
     4081 catgccgccc tcctgggcga tggcgatcgc gagccgcgcc tcggtcaccg tgtccatcgc
     4141 ggcggacacc agcggcaggt tcagctggat attgcgtgag atccgggtgg caagactggt
     4201 gtcgcgtggc aacacctggg agaacgctgg caccaacaac acatcatcga aggtgagcgc
     4261 tttaccaaga aggcgcatgg aaatggctcc tgacgcaaaa cgaaattata gggatgcagg
     4321 aacagatgca cggtttgggc cgcgcattgc gtcgggtgac gcaagatggc acgcttacac
     4381 tttcagtgga cttgatcgag gtgcccttcc catgcgtcac tggcctgctg cgttgtttgg
     4441 aatcgttctg ctgagcagcc tgtgcctgcc cgcagctgcc cagtggaaat ggcgcgacaa
     4501 gaacggtcag acgcagtaca gcgatctgcc cccaccacag ggaacgcctg catcagccgt
     4561 actgcagcgg ccgaccggcg ccaagctgcg cagcgtgctc cccgcagacg ccgaggctgc
     4621 tgcatcggcg cccctcgcac ccgcccccaa gaccgtggag cccgagctcg aggccaagct
     4681 gcgcaaggcc gaacaggaaa aagctgcaaa gtccaaggct gaagacgaga agatcgccct
     4741 gcagaaggcc gagaactgca gccgggctcg caatcagatg cgggtgatcg acgaaggatt
     4801 gcgtgtcgcc cggatcaacg agaagggtga gcgcgaggtg ctcgacgaca agggccgcgc
     4861 cgaagagggc aaccgggccc gggccatcat cgcttccgac tgcaagtagt tcgttgccgg
     4921 cgccgccggg cgtccttggg gtcgatcagc aacgggcggt agacctcgac ccgatcgtga
     4981 tcgcgcagcg ggtgatcgaa cggcttcagc tgaccccata cgccgacctt caatctcgac
     5041 atcccgaagt cgacgccttg cggaagcagc ccgctgaggc gcaaggcgtc tgcgacccgt
     5101 gaacctgcag gcaggctgag cctgatccga cgcatttcac cggcagcggg actgtggacc
     5161 acctcgatct ggagcacctc ggcctcagcg gtctccatag acctgcgccg cgcgcttgac
     5221 gaaggattcg acgaaggtat tcgcgatgcg atcgaagacc gggctcacga ccgcttcgag
     5281 cgccacgctc gcgaaggcgt agcgcaactc gaactcgatc ttgcaggcct gggcctccga
     5341 tccgacgcga tgaaacagcc attgcccgtc gagctgcgag aacgggccat cgaccagctg
     5401 aacgcgcacc gcactgtcgc tgtcgtgctc gttgcgggtg gtgaacgcat gccggatacc
     5461 ggcgtacgcc agatgcaacc gggcggtcat gctggcatcg gtgtgttcga gaacctcgga
     5521 acgggcgcac cagggaagga acttcgggta gtcggccacc ccggtgacca gttcgtacat
     5581 ctcgtgcggc gagtaccaca gcagcactga tttcttgacg tgcttcatac aatgcgccga
     5641 ttgtattgac gtgcccagag cgcgacccct tcatgcccat cgccgagaac cgac
//
LOCUS       DAOORV010000208         5684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17159_length_5684_cov_11.748623, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000208 DAOORV010000000
VERSION     DAOORV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17159_length_5684_cov_11.748623"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKV56_17170"
     CDS             complement(<1..642)
                     /locus_tag="PKV56_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOX69587.1"
                     /translation="MTLSQARWHVIAQSNFAWEREALDWLRSHLPDRDPWHVWTNFEF
                     IDDEGKVSEVDALVLSPAGLFLVEIKSRPGVLTGDAHSWTWTTDGRTNTYDNPLILAN
                     RKAKRLASLLKRQPAIAKAKIRLPFVEPAIFLSSSSLACRLEGIAKSATFQHGRPGSV
                     DDDGIVGALANGIANRGATTVDAQQARAIARGLNEAGIRPSNKHRQVGDYKLGK"
     gene            complement(639..860)
                     /locus_tag="PKV56_17175"
     CDS             complement(639..860)
                     /locus_tag="PKV56_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX69588.1"
                     /translation="MIRCHLSSLMGRDKLNISDVARATGLNRSTITALYRETATRIEL
                     PAIEQLCALFKCSVGDLLEVVPDNQKGTP"
     gene            complement(964..1752)
                     /locus_tag="PKV56_17180"
     CDS             complement(964..1752)
                     /locus_tag="PKV56_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69589.1"
                     /translation="MDSTPAESMDLRDRLNAITAHQLGGRGRFKQLAEASGIGMDSWK
                     NTWHNKQRPTTEMVQFVARAWPQYAFWLVTGITDARHGHQAPDECDTYPENFRTSRPG
                     AEPYFRHAVDMLERRERGEPELLTDLVELRALATARANDEISAEAAETMRDGAAIAAT
                     VQDLDTDTPPKAETLTGRLLAELQSGRQLTDQQMARLLGVEEETFLEVKSDTSKIKRL
                     AMGRIFDAWAYDAIRDALLKVAPDRFAQAIRQRDVERGRRMLGR"
     gene            1877..2221
                     /locus_tag="PKV56_17185"
     CDS             1877..2221
                     /locus_tag="PKV56_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69590.1"
                     /translation="MLSMTNLEQMVRNAETAGEAATHAARQVLLDCLLRSQPEVMTLE
                     NLGQLLQIPVNTLRADLHRRPETMPRRLYLPGRRLVRFLRVDVAVWLLSSRSNDGVIV
                     DAANRVASRGTL"
     gene            2242..2469
                     /locus_tag="PKV56_17190"
     CDS             2242..2469
                     /locus_tag="PKV56_17190"
                     /inference="COORDINATES: protein motif:HMM:NF025352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HOX69591.1"
                     /translation="MATETLNADELVAITGCGRRGDQVAWLQAAGWTYVTNRAGEPIV
                     GRLYARLRLAGITPSALMATGGWAPDFSSVR"
     gene            2519..3550
                     /locus_tag="PKV56_17195"
     CDS             2519..3550
                     /locus_tag="PKV56_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69592.1"
                     /translation="MLRRKKTYVSGRTWTAYYYNGRDAEGRRIEVPLGTDLNEAKRKW
                     AELDCKPPPRETGLMAFVFDRYEREIVPLRAPATQRDYIANLRQLSKVFGNAPVDAIT
                     PQHVAQYRDRRGAKVRANRELALLSHVFNLAREWGYTAKDNPVRGVRKNKEAPRDFYV
                     DEPVWSAVYACAPIELQDAMDLNYLSGQRPADVLKMSVTDIKEGALEVRQGKTKKRLR
                     ITLDLPDGTRTELGQLLDRIRARPRKVSSLFLVATPAGVPLNRWTLRIRFDAARKAAA
                     EAAERDGTPEGVALAARIRSFQFRDIRPRAASDIGDLAAASKLLGHTEQQITRKVYIR
                     VGETVKPTK"
     gene            3755..4396
                     /locus_tag="PKV56_17200"
     CDS             3755..4396
                     /locus_tag="PKV56_17200"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69593.1"
                     /translation="MAPDVRKVAPVYMATQAIRLSGVANDSACATRAGSAARQRRVVT
                     VADARSAARCGRPAFMRPPSSRAITIAVANFKGGVGKTTTAMALAQGLSLHGHRVLAI
                     DLDPQGSLSTLFGIVAQTEVNEAMTALPLLRGECDDLLPVARATYWDGVDLVPAAPML
                     FAAEFAIPSRHAQSSGESFWTLLDRGLAAARKAYDCWRRPKIDQLTRVVPTEN"
     gene            4456..>5684
                     /gene="istA"
                     /locus_tag="PKV56_17205"
     CDS             4456..>5684
                     /gene="istA"
                     /locus_tag="PKV56_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOX69594.1"
                     /translation="MALKGKIRALHQREGKSISEIARRTSLSRNTIKKWLKAPAEAAP
                     KYRRESPPGKLAPFVATLTQALKADAHRPRHERRTARALLLQLQAQGYDGGYSRLTDF
                     IRAWRLGEGQAVLSTAFVPLRFELGDAFQFDWSEEGLVVGGIYYRLQVSHLKLCASRA
                     FWLVAYPSQGHEMLFDAHTRSFTALGGIPRRGIYDNMKTAVDKVKKGKGRIVNARFSV
                     MCNHYLYDPDFCNVASGWEKGVVEKNVQDSRRRIWIEAGERRFGSFVELNAWLAERCR
                     SLWAEIRHPEHKQFSVAEMLEFEQPHLMPMPEPFDGYVEAPARVSSTCLVAVARNRYS
                     VPCELAGHQVSTRLYPGRVVAVADDTVVASHERLSDRGQTRYDWQHYIPLVQRKPGVL
                     RNGAPFADMPEPLIQLQR"
BASE COUNT          996 a         1734 c         1967 g          987 t
ORIGIN      
        1 cttgccgagc ttgtagtcgc cgacctggcg gtgcttgttg gacgggcgga tgccggcctc
       61 gttgaggccg cgcgcaatgg cacgggcctg ctgggcatcg accgtggtgg cgccacggtt
      121 ggcaatgcca ttggcaaggg cgccgacgat gccatcgtca tcgacggagc caggccggcc
      181 atgctggaaa gtggcgctct tggcgatgcc ttcgagccgg caggccaggc tgctggaaga
      241 caggaagatc gccggttcga cgaaggggag ccgaatcttg gccttggcga tcgccggttg
      301 gcgcttcagc aggctggcca ggcgtttggc tttgcggttg gccaggatca gcgggttgtc
      361 gtacgtgttg gtgcggccgt cggtggtcca agtccagctg tgggcgtcgc cggtcagcac
      421 gccgggccgg ctcttgattt cgacaaggaa taggccagca ggtgagagca cgagcgcatc
      481 gacttcgctg actttgccct cgtcgtcgat gaactcgaag ttcgtccaga cgtgccaggg
      541 gtcgcggtcg ggcaggtgac tgcggagcca gtcgagggct tcgcgctccc aggcgaagtt
      601 gctttgggcg atgacgtgcc agcgggcttg gctgagcgtc atggggtgcc cttttggttg
      661 tcaggcacga cttccagcag gtcgccgacg gagcacttga acagcgcgca cagctgctcg
      721 atggcgggca gctcgatgcg ggtcgcggtc tcgcggtaca gcgcggtgat cgtgctgcga
      781 ttgaggccgg tggcgcgggc cacgtcggaa atgttgagct tgtctcgacc catgagggac
      841 gagagatggc agcggatcat ggcgaccgtt ggctgtcggg gtggtgcgca ggatagcaaa
      901 ttgaattgca gcgcacaacc cgtggcggtg ccggcgagcg gctaatggtc taccgggggt
      961 gtttcaacgc ccgagcatgc gtcggccgcg ttcgacatca cgctgtcgga tcgcttgggc
     1021 aaatcggtcg ggcgcgacct tgagcagcgc gtcgcggatc gcgtcgtagg cccaggcatc
     1081 gaagatccgc cccatcgcca gccgcttgat cttcgaggtg tcggacttca cctcaagaaa
     1141 ggtttcctct tccacgccca gcagtcgggc catctgctgg tcggtgagtt gtcggccgga
     1201 ctgcaactcg gcaagaaggc gccccgtgag cgtctcggcc ttgggaggag tgtccgtatc
     1261 cagatcctga accgtggcgg ctatggcggc gccatcgcgc atggtctcgg cggcctcggc
     1321 ggaaatctcg tcattggctc gcgcggtggc caatgcccgc aactcgacca ggtcggtcag
     1381 caactcaggc tcgccgcgct cgcgccgctc cagcatgtcg acggcatgtc ggaagtaggg
     1441 ttctgcgccg ggtcgagagg tgcggaagtt ctccgggtac gtgtcgcact cgtcaggtgc
     1501 ctgatgcccg tgacgggcgt cagtgatgcc ggtgacgagc cagaacgcgt actgaggcca
     1561 tgcccgcgcg acgaactgga ccatctccgt ggtcgggcgc tgcttgttgt gccaagtgtt
     1621 cttccaggag tccatgccga tgccggaggc ctccgccaac tgcttgaacc tgccccttcc
     1681 ccccagctga tgcgcggtga tcgcgttcag gcggtcccgg aggtccatcg actcggctgg
     1741 tgtgctgtcc atacccgaat tctacggaat tggagcttca tgaccacgtt tagctgtgat
     1801 cgcggtgagt ttttgacgcg aatggcttgc acgcgacgca gaaaggtgta cgattaacac
     1861 ataacgagat caaaacatgc tttctatgac caaccttgag caaatggtac gcaatgcgga
     1921 gacggctggc gaggccgcga cgcatgccgc gcgtcaggtg ctgctggact gtctgctgcg
     1981 cagtcagcct gaggtgatga cgctggagaa cctgggccag ttgctccaaa tccctgtgaa
     2041 caccctccgc gccgacctgc atcgccgccc cgaaacgatg cctcggcggt tgtacctgcc
     2101 gggccgccga ctcgtgcggt tcctgcgtgt cgacgtggcg gtgtggctcc tgtccagcag
     2161 gtcgaacgat ggcgtcatcg tggacgccgc caatcgtgtc gcttcgaggg gaaccctgtg
     2221 accgctttgt tcgagatgcc catggccacc gagactctga acgccgacga actggtcgcg
     2281 atcacgggtt gcggccggcg cggcgatcag gtcgcgtggc tgcaagccgc agggtggaca
     2341 tacgtgacca accgagccgg cgagccgatc gtgggccgtc tctacgcgag gctgcgcctc
     2401 gcaggcatca cgccaagtgc cttgatggcc accggcggct gggcgcctga cttctcgtcg
     2461 gttcggtgag ggggttgcca tgcggccgaa atccactgcc cgcgacctcc cgccccggat
     2521 gcttcgtcga aagaagacgt acgtctcggg tcggacttgg actgcctact actacaacgg
     2581 ccgcgacgcc gagggcaggc gcatcgaggt gcccctgggc accgacctca acgaagccaa
     2641 gcgcaagtgg gccgaactgg actgcaagcc gccgccgcgc gaaacaggcc tgatggcctt
     2701 cgtcttcgac cgctacgagc gcgagatcgt gcctctgcgc gcgcctgcga cgcagcggga
     2761 ctacatcgcc aacctgcgcc agttgtcgaa ggtgttcggc aacgcgccgg tggacgcgat
     2821 cacgccgcag cacgtggccc agtatcgcga tcgccgcgga gccaaggtgc gtgcgaaccg
     2881 cgagctggca cttctgtcgc atgtgttcaa cctcgcccgc gaatggggat acacggcgaa
     2941 agacaacccg gtcagaggcg tccgcaagaa caaggaagcg ccgagggact tctacgtcga
     3001 cgagccggtg tggagcgcgg tgtatgcgtg tgcaccgatc gagctgcagg acgcgatgga
     3061 cctcaactac ctcagcggcc agcggcctgc cgacgtgttg aagatgagtg ttacggacat
     3121 aaaagaaggt gcgctggaag tgcgccaggg caagaccaag aagcgccttc ggatcacgct
     3181 ggacttgccc gatgggaccc gtacggagct tggccagttg ctggaccgga ttcgggcacg
     3241 tccgcgcaag gtcagcagtt tgtttctcgt cgcaacgccg gccggcgtgc cgctgaatcg
     3301 atggacgctg cgcattcgat tcgacgcggc acgaaaagcc gccgccgagg ctgccgagcg
     3361 ggatggcacg cctgaaggcg tggcactcgc cgcacgcatc cgcagctttc agttccgcga
     3421 catccgtccg cgggctgcga gcgatatcgg agatctggcg gcggccagca agttgctcgg
     3481 gcatacggag cagcagatca cgcgcaaggt ctacatccgt gtgggcgaga cggtgaagcc
     3541 gaccaagtaa ccggtgtcgt tggcagtcaa cacaccgaat ccgcgaacga acgaggaagg
     3601 cgaagcgtcc atgcgaccaa tcgacacgtc agagcttgga aacaaggttt ccgctgcggc
     3661 agaagcaaag ccgccgcggc ccatcagcat ggacgagatc gcatcggtgg cagatcgcgc
     3721 tgacgcgctg gcgcacgaga ttcgagcccg tgtgatggcg ccggacgttc gaaaggtggc
     3781 gccggtgtac atggccaccc aggcgatccg gttgtctggt gtcgccaacg attccgcgtg
     3841 cgctacgcgg gcaggctctg ctgcccgtca acgacgggtg gtgactgtgg ctgatgcgcg
     3901 aagcgcagca cgctgcggca ggcctgcgtt catgcgccca ccgtcaagca gggcgatcac
     3961 catcgccgtc gccaatttca agggtggcgt cggcaagacc acgacagcga tggcattggc
     4021 gcagggcctc agcctgcacg gccacagagt cctggccatc gatcttgatc ctcagggctc
     4081 gctgagcacg ctgttcggaa tcgtggctca gaccgaggtc aacgaagcca tgactgcgct
     4141 gcccttgctg cgtggcgagt gcgatgacct gcttcccgtt gcacgagcaa cctactggga
     4201 cggcgtggac ctggtgcctg cggcgccgat gttgttcgcg gccgagttcg ccataccaag
     4261 tcggcacgcg caaagcagcg gcgagagctt ctggacgctg ctggaccgtg gccttgcggc
     4321 ggcgcgcaag gcatacgatt gttggcgccg accgaaaatt gaccagctaa cccgcgtagt
     4381 tccgaccgaa aactgaccag ggtgaacgac ctactctgct gcaatttgtg gcaggggatt
     4441 tggacggtga tcaccatggc cttgaagggc aagatccggg cgctgcacca gcgcgagggc
     4501 aagtcgatca gtgagatcgc caggcgcacg agcctgtcac gtaacacgat caagaagtgg
     4561 ttgaaggcgc ccgcggaggc ggcgcccaag tacaggcgag agtcaccgcc aggcaagctg
     4621 gcgccgtttg tggccacgct cacgcaggcg ttgaaggctg acgcgcaccg gccacgccat
     4681 gagcggcgca cggcacgcgc gctgctgctg caactgcagg cccagggcta cgacggcggc
     4741 tacagccggc tgacagactt catccgggcc tggcgcctgg gcgagggcca ggcggtgctg
     4801 agcacggcct tcgtgccgct gaggtttgag ctgggcgatg ccttccagtt cgactggagc
     4861 gaagaaggac tggtggtggg cggcatttac taccgcctgc aggtctcgca cctgaagctg
     4921 tgtgcctcac gggccttctg gctggtggcc tatcccagcc agggccacga gatgctgttc
     4981 gatgcgcaca cccgctcgtt caccgcgctg ggcggtatcc cgcgccgtgg catctacgac
     5041 aacatgaaga cggcggtgga caaggtcaag aagggcaagg gccgcatcgt caacgcccgc
     5101 ttctcggtga tgtgcaacca ctacctgtac gacccggact tctgcaacgt ggcctcgggc
     5161 tgggagaagg gggtggtgga gaagaacgtg caggacagcc ggcggcgcat ctggatcgag
     5221 gccggtgagc ggcggttcgg ttcgttcgtg gagttgaacg cctggctggc cgagcggtgc
     5281 cggtcgctgt gggcggagat ccgccacccc gagcacaagc agttcagcgt ggccgagatg
     5341 ctggagttcg agcagcccca tctgatgccc atgcccgagc cgttcgacgg gtacgtggag
     5401 gcaccggcgc gggtgtcgag cacctgcctg gtcgcggtgg cgcgcaaccg ctactcggtg
     5461 ccgtgtgagc tggccggcca ccaggtcagc acccggctgt accccggccg cgtggtggcg
     5521 gtggccgatg acacggtggt ggccagccac gagcggctca gtgaccgggg tcagacgcgc
     5581 tacgactggc agcactacat cccgctggtg cagcgcaagc ctggggtatt gcgcaacggc
     5641 gcgccgttcg ccgacatgcc cgagccgctg atccagctcc agcg
//
LOCUS       DAOORV010000209         5680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17172_length_5680_cov_6.100267, whole genome shotgun sequence.
ACCESSION   DAOORV010000209 DAOORV010000000
VERSION     DAOORV010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17172_length_5680_cov_6.100267"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PKV56_17210"
     CDS             complement(<1..1863)
                     /locus_tag="PKV56_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HOX69595.1"
                     /translation="MAEPLESGPFRRRLDRIYLAYTASFILFVLALAMLERMGMPKHW
                     IGVTFLLSTVLLYAGIGILSRTTDPTEYYVAGRRVPAFYNGMATAADWMSAASFIGLA
                     GTLYLQGYAGLAFVLGWTGGYCLVALLIAPYLRKFGQFTIPDFLGARYGGNLPRAVGA
                     FAAVLCSFVYLVAQIYGVGLITARLTGVDFVIGIFLGLGGVLVCSFLGGMRAVTWTQV
                     AQYIILIIAFLVPVMWLSVKQTGVPIPQLVYGAQLAKVTEREKQLLNDPRETEVIAAF
                     KARAEDYAARLVDVPKALAAERDAGRQKVAALKADKAPLADIQAAEKELLRIPKTEAA
                     AREAWTRAVASNEARARPLAGMPPHGQQFAGDPKGSEAERELFGLSRMNFLALVLCLM
                     VGTAGLPHILARCYTTPSVREARRSVTWALFFIFLLYMTVPALAVMVKFEVFNVLVGT
                     RFDQLPAWMSTWSRVDPSLLSVVDINKDGILQLGEMRIGGDIVVLATPEIAGLPYVIS
                     GMVAAGGLAAALSTADGLLLTIANALSHDFYFKMVNPGASAARRVALSKVTLLAVALG
                     AAAVAAQRPADIVFLVSAAFSLAAAAFFPALVLGVFWSRANQPGALAGMVAGLAV"
     gene            complement(1866..2327)
                     /gene="rnhA"
                     /locus_tag="PKV56_17215"
     CDS             complement(1866..2327)
                     /gene="rnhA"
                     /locus_tag="PKV56_17215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOX69596.1"
                     /translation="MTESAPKITRPVVTVYTDGACKGNPGRGGWGAWLASEGHEKELF
                     GGEALTTNNRMELTAVIESLASLKRTCSVVIYTDSEYVRKGITEWIHGWKQRGWKTAD
                     RKPVKNAELWQRLDALRSLHHVEWRWVKGHSGDPGNERADALANRGVEAAS"
     gene            complement(2324..3211)
                     /locus_tag="PKV56_17220"
     CDS             complement(2324..3211)
                     /locus_tag="PKV56_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX69597.1"
                     /translation="MTETATSISLGAWFATPPGAYLLDWEQRHLDEAVADVFGFHALQ
                     LGLPQLDALRGNRMPHRWLAVDTPHRSDVLGAAKPPVPRGVTLNCDFDALPFDSQSLD
                     LVVLPHALELARDPHQTLREVERVLMPEGRVVIIGFNPNSLWGMRQQTGHARRQLGLG
                     LGKSMFLSRERDLIAYRRLRDWLRLLSFEVETGRFGCYRPPMRSDKWLSRFAWVESAG
                     ARWWPVFGAVYALTAVKRVRGMRLVGLVREQRPKAHAAPAVVARNQPHLQDTARWSRE
                     RAEPACSDGFREQEPSDVR"
     gene            3210..3998
                     /gene="gloB"
                     /locus_tag="PKV56_17225"
     CDS             3210..3998
                     /gene="gloB"
                     /locus_tag="PKV56_17225"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOX69598.1"
                     /translation="MNLIALPAFTDNYIWLLHDGIDAVVIDPGDCAPVLAALDRLRLR
                     LEAILVTHHHDDHVGGVTGLQSRLQGSVYGPAFETMPEPCTRLRGGETVQVLGLDFDV
                     IDVPGHTSGHIAYFHQPKSQREPPIEAPLLFCGDTLFSGGCGRLFEGTPAQMHHSLSA
                     LAALPGDTRVCCAHEYTLANLKFAQAVEPDNGDLDRYTRWCEQQRAAGLPTLPGRVAD
                     ERAINPFLRCDVPAVIDAACRHGARSDRPADVFAALRLWKNEFR"
     gene            3995..5629
                     /locus_tag="PKV56_17230"
     CDS             3995..5629
                     /locus_tag="PKV56_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOX69599.1"
                     /translation="MTCKNLLAPAARRLTLGLALAVAGWCSGSGCATHPAGPASTTTV
                     GSAGAAPAPELPAQQASAAGQEAAPPSALADESPPAAAVDPMRPDISVNLDDAAARTD
                     LWARVRRGFGMSDLDNELVRDRERWYASRPDYVDRMTTRGSRYLFHIVEELESRGMPT
                     ELALLPFIESAFNPQAMSSAKASGMWQFMPATGRDFALKQNIFRDDRRDVLASTRAAL
                     DYLQRLHGMFGDWHLALAAYNWGEGNVQRAIARNEKAGRPTDYASLKMPEETRFYVPK
                     LQAVKNIVSNPQAYSLTLPALANHPYFLGVAIKRDIDVELAARLAGMSLEDFKTLNPQ
                     MNKPVILAAGTPQVLLPYDNASSFVRKLSEHRAPLATWTAWVAPRTMRPADAARLVGM
                     SESQLREINRIPPRMLVKAGSTLLVPRSEQRHTDVAGHVADNATMLLAPDVPPLRRVV
                     LKAGKADSVASIARRYRVGAAQVARWNSVGTGTRFKPGATIVVYEARRNAASSRAATG
                     GKAKKPVSVTAAAKKKSSRMAQKKTSTRKAVRLAAD"
BASE COUNT         1042 a         1932 c         1823 g          883 t
ORIGIN      
        1 gaccgcaaga ccagcgacca tgccggccag ggcgccgggc tggttcgcgc ggctccagaa
       61 caccccgagc accagcgccg ggaaaaatgc cgccgccgcc agcgaaaacg ccgcggacac
      121 gagaaagacg atgtcggcag ggcgctgcgc ggccaccgcc gccgcgccga gcgccacggc
      181 aagcagcgtg accttcgaga gcgcgacgcg acgcgcggcg gaggccccgg gattgaccat
      241 cttgaaatag aagtcgtgcg acagcgcgtt ggcgatcgtc agcagcaggc cgtctgcggt
      301 ggacagcgcg gcagcgaggc cgccggcggc gaccatgccg gagatcacgt acggcaggcc
      361 ggcgatctcg ggagtcgcca gcacgacgat gtcgccaccg atgcgcatct cgccaagttg
      421 aagaatgccg tccttgttga tgtcgacgac cgaaagcaat gaagggtcga cccgcgacca
      481 ggtcgacatc caggcaggca gctgatcgaa ccgcgtgccg acaagcacgt tgaagacttc
      541 gaacttgacc atcaccgcga gggccggaac cgtcatgtac agcaggaaga tgaagaaaag
      601 cgcccaggtc accgaacgcc tggcctcccg caccgatggc gtggtgtagc agcgcgccag
      661 gatgtgcggc aagcccgcgg tgccgaccat caggcacagc accagcgcca ggaaattcat
      721 gcgcgagaga ccgaagagtt cgcgctcggc ctcgctgccc ttcgggtcgc cggcaaattg
      781 ctggccgtga ggcggcatgc ctgccagagg gcgcgcccgc gcctcgttgg aagccacggc
      841 acgcgtccat gcctcgcgtg cagcggcctc ggtcttcggg atgcgcagca attccttttc
      901 agccgcctga atgtcggcca gcggggcttt atcggccttc agggcggcga ccttctgccg
      961 gccggcatcg cgctcggccg ccagggcctt cgggacatcg accagccggg cagcgtaatc
     1021 ctcggcacgc gccttgaatg ctgcgatgac ttccgtttct ctcgggtcat tcaacaactg
     1081 cttctcgcgt tcggtcacct tggcgagctg tgcgccatag accagctgcg gaatcggcac
     1141 gcccgtctgc ttgaccgaca gccacatgac cggcaccagg aaggcgatga tgagtatgat
     1201 gtactgggcc acctgcgtcc aggtcaccgc gcgcatgcct ccgaggaaag agcacaccag
     1261 tacgccgccc agaccgagga agatgccgat cacgaaatcg acccctgtga ggcgggccgt
     1321 gatcagcccg acgccataga tctgggcgac caggtagaca aacgagcaga gcacggcagc
     1381 aaacgcaccg actgcacgcg gcagattgcc gccgtaacgc gctccgagga aatccgggat
     1441 cgtgaactgg ccgaacttgc gcagatacgg cgcgatcagc aaggccacaa ggcagtagcc
     1501 gccggtccag ccgagcacga aagccagtcc ggcgtagccc tggaggtaga gcgtgccggc
     1561 cagcccgatg aacgacgccg cagacatcca gtcggccgcc gtcgccatgc cgttgtagaa
     1621 ggctgggacg cgccggccgg cgacgtagta ctcggtcggg tcggtggtgc gactcaggat
     1681 gccgataccg gcatagagca gcacggtgga cagcaggaac gtgaccccga tccagtgctt
     1741 gggcatgccc atgcgctcga gcatcgccag cgccagcacg aacaggatga aactggccgt
     1801 gtaagccaga tagatgcggt cgagccggcg cctgaacggc ccgctttcaa gcggctcggc
     1861 cacggtcagg aagcggcctc cacaccgcga ttggccagtg catcggcacg ctcattgccg
     1921 gggtcgcccg aatgcccctt gacccagcgc cactcgacgt ggtgcagtga ccgcaacgca
     1981 tccagtcgct gccacaactc ggcgttcttc acgggtttgc ggtcagcggt cttccagccc
     2041 cgctgcttcc agccgtggat ccactcggtg attcccttgc gcacatattc gctatcggtg
     2101 tagatcacga ccgaacaggt ccgcttgagg gatgccagcg attcgatgac ggcggtcagt
     2161 tccatgcggt tgttggtggt cagcgcttcg ccgccgaaga gttccttctc gtgaccctcg
     2221 ctcgccagcc aagcccccca gccgccccgc cccgggttgc ccttgcaagc accatcggtg
     2281 tacacggtga ccacggggcg cgtgatcttc ggtgctgatt cggtcatcga acgtcactcg
     2341 gctcttgctc gcggaaaccg tcggaacaag ccggttcggc ccgttctcgg gaccatctcg
     2401 cggtgtcctg gagatgcggc tgattcctcg cgacgacagc cggtgcggcg tgcgccttcg
     2461 gccgctgttc gcgcaccagc ccgaccagcc gcatgccgcg cacccgcttg accgcggtca
     2521 gcgcgtagac cgcgccgaac accggccacc agcgggcgcc agccgactcc acccacgcga
     2581 agcgcgacaa ccacttgtcg gatcgcatcg ggggccgata gcagccgaag cgtcctgtct
     2641 cgacctcgaa gctcagcaat cgcaaccagt cgcgcaggcg acggtaggcg atcagatcgc
     2701 gttcgcgcga caggaacatc gatttgccca gccccagccc cagctggcgc ctggcatggc
     2761 cggtctgctg gcgcatgccc cacaggctgt tggggttgaa tccgatgatg acgacccggc
     2821 cctcgggcat cagcacccgc tcgacttcgc gcagggtctg gtggggatca cgcgccagct
     2881 cgagcgcatg aggcaggacc accagatcga gactctgact gtcgaagggc aaggcatcga
     2941 aatcgcagtt caacgtgacg ccgcggggca caggcggctt ggccgccccg aggacgtcgg
     3001 agcgatgcgg cgtatccacg gcgagccagc gatggggcat gcggtttccg cgcaaggcgt
     3061 cgagttgagg caggcccagc tgcaatgcat gaaagccgaa tacatcggcc accgcctcgt
     3121 cgagatgtcg ctgctcccag tccagcagat aggcgccggg cggggtcgcg aaccaggccc
     3181 ccaaacttat actcgttgcg gtttccgtca tgaatttaat tgcactgccc gcgttcaccg
     3241 acaactacat ctggctgctt cacgatggca tcgacgccgt cgtgatcgac ccgggagatt
     3301 gtgcgccggt gcttgcggcc ctcgaccgcc tgaggctccg gctcgaagca attctagtga
     3361 cgcaccatca cgacgatcac gtgggtgggg tgacgggcct gcagtcgcgc cttcagggct
     3421 ccgtgtacgg tcccgccttc gagacaatgc ccgagccctg cacccgcctt cgtggcggcg
     3481 agacggtgca ggtgctcggg ctcgacttcg atgtgatcga cgtgcccggg cacacttcgg
     3541 gccacatcgc ctacttccac caaccgaagt cgcagcgtga gcctcccatt gaagcgccgc
     3601 tgcttttctg cggtgacacg ctgttttctg gcggttgcgg acgtctcttc gaaggcacgc
     3661 cagcgcagat gcaccactcg ctgagcgcgc tggcggccct gcctggcgac actcgcgtgt
     3721 gctgcgccca cgagtacacg ctggccaacc tgaagttcgc ccaagcggtc gagcccgaca
     3781 atggtgatct cgaccgatac acccggtggt gcgaacaaca gcgcgctgcc ggcttgccca
     3841 ccctccccgg ccgcgtggcc gatgagcgtg ccatcaatcc cttcctgcga tgcgatgtgc
     3901 ctgcggtgat cgatgcggca tgccgacatg gcgcgcgaag cgatcgaccg gcggatgtgt
     3961 tcgcggcgct gcgactctgg aagaacgaat tccgatgacc tgcaagaact tgcttgcgcc
     4021 cgctgcgcgc aggctcacac tgggcctcgc gctcgccgtt gccgggtggt gctccggttc
     4081 cggctgcgcc acgcacccgg caggccctgc ctcgacgacc accgtgggct ccgctggcgc
     4141 agcgccagcc cccgagctgc cggctcaaca ggcctccgcc gcgggtcagg aagctgcgcc
     4201 tccatccgca ctggccgatg agtcgccgcc ggccgcggcg gtcgacccga tgcgtccgga
     4261 catcagcgtg aaccttgacg atgctgccgc ccggaccgac ctctgggcgc gagtccgccg
     4321 cgggttcggc atgtccgacc tggacaacga gctcgtgcgc gacagggagc gctggtacgc
     4381 cagccggccc gactacgtcg accgcatgac gacgcgcggc agccgctacc tcttccacat
     4441 cgtcgaggag ctggagtcac gcggcatgcc cacggagctg gcactgcttc cattcatcga
     4501 aagcgcgttc aacccgcagg ccatgtcgtc ggctaaggct tctggcatgt ggcagttcat
     4561 gcctgccacc ggtcgggact tcgcgctcaa gcagaacatc tttcgcgacg accggcgcga
     4621 tgtgctggcc tcgacgcgcg ccgccctcga ttacctgcag cgtctgcacg gcatgttcgg
     4681 cgactggcac ctggcactgg ccgcctacaa ctggggcgaa ggcaacgtgc agcgtgcgat
     4741 tgcccgcaac gagaaggccg ggcgccccac cgactatgcg agcctgaaga tgccggagga
     4801 gacgcgcttc tacgtgccca agctgcaggc cgtcaaaaac atcgtctcga atccgcaggc
     4861 ctactcgctg acgctgcccg cgttggccaa ccacccgtat tttctgggcg tggcgatcaa
     4921 gcgcgacatt gacgtcgagc tggccgcacg tctcgcgggc atgtcgctcg aagacttcaa
     4981 gacgctcaac ccgcagatga acaagccggt gatcctggct gccgggacac ctcaggttct
     5041 gctgccgtac gacaacgcca gttccttcgt gcgcaagctc tcggagcacc gcgcaccgct
     5101 ggcgacctgg accgcctggg tggcacccag gacgatgcgc ccggcagacg ctgcacgcct
     5161 ggtcggcatg agcgaatcac agctgcgtga gatcaaccgc atcccgccgc gcatgctggt
     5221 caaggcagga tcgaccttgc tcgtgccgcg cagcgagcag cgccataccg atgtggctgg
     5281 gcatgtcgca gacaacgcga cgatgctgct ggcgccagac gtgccgccgc ttcgtcgcgt
     5341 ggttctgaag gcaggcaagg ccgattcggt agcgagtatc gcgaggcggt accgagttgg
     5401 agcggcacag gtcgcccgat ggaactccgt gggcaccggc acccgcttca agcccggtgc
     5461 gacgatcgtc gtctacgagg ccaggcgcaa cgccgcttcc tcgcgagcag ctacaggggg
     5521 caaggcgaag aaacctgtat cggtgactgc tgcggccaag aagaagtcca gcaggatggc
     5581 acagaagaag acctcgaccc gcaaggcagt gcggctggcc gcggactgat cagacgcccg
     5641 agaacaacag cgccacgttg acggccagcg ccgcaatcca
//
LOCUS       DAOORV010000210         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17207_length_5672_cov_7.547623, whole genome shotgun sequence.
ACCESSION   DAOORV010000210 DAOORV010000000
VERSION     DAOORV010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17207_length_5672_cov_7.547623"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKV56_17235"
     CDS             complement(<1..302)
                     /locus_tag="PKV56_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOX69600.1"
                     /translation="MSSLSSSVPKRKFDVVIVGAGGSGMRASLQLARAGLNVAVLSKV
                     FPTRSHTVAAQGGIGASLGNMSEDNWHYHFYDTVKGSDWLGDQDAIEFMCREAPKVV"
     gene            complement(323..685)
                     /gene="sdhD"
                     /locus_tag="PKV56_17240"
     CDS             complement(323..685)
                     /gene="sdhD"
                     /locus_tag="PKV56_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOX69601.1"
                     /translation="MAQNFGSKRIVVGAHYGLRDWLSQRVTAAVMALFTLAVIVQILL
                     PGEMGYDKWAGIFSQQWMKVLTFVVIVSLLYHVWVGMRDVWMDYVKPVGIRLALQVFT
                     IVWLVGCAGWAIQVLWRL"
     gene            complement(696..1118)
                     /gene="sdhC"
                     /locus_tag="PKV56_17245"
     CDS             complement(696..1118)
                     /gene="sdhC"
                     /locus_tag="PKV56_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOX69602.1"
                     /translation="MSETLKPRPTHRNIHISQIVKYRLPPAGIVSILHRVSGVGMFLL
                     MPFIIWMFDTSVTSEISYSQFTSVFTGGLGILPGWVFKLIALGLTWAFLHHFIAGVRH
                     LWMDMTHAVTKEFGHSSALATLGLSLLLTALLGSKLFF"
     gene            complement(1241..2020)
                     /locus_tag="PKV56_17250"
     CDS             complement(1241..2020)
                     /locus_tag="PKV56_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69603.1"
                     /translation="MKNSSALAVEPLPAARPGDGTSPSFSPLYQQIKTLMTRSLRMGE
                     WRPGEAIPSEMELAARFKVSQGTVRKAIDELAAENLLVRRQGKGTFVATHAEQKVQYR
                     FLRLMPDDGESGTVQRRFIDCRRLRSTADVARALAMSPGDSVVNVRRVLTFNSTPVVL
                     DDIWLPGLLFKGLTAERLSDYRGPMYGLFESEFGVHMIRADEKIRAVTADPASAQLLA
                     VDVGFPLLSVERLSLTYDDKPVELRRGLYNTSRYHYRNALS"
     gene            2241..3227
                     /locus_tag="PKV56_17255"
     CDS             2241..3227
                     /locus_tag="PKV56_17255"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOX69604.1"
                     /translation="MSKSPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQNALKGVIMELDDCAFPLLAGIEAHSDPVAAFKNTDYALLVGARPRGPGMERS
                     DLLAANAQIFTAQGKALNASASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
                     LDHNRAASQIAAKTGKPVSSIQKLAVWGNHSPTMYADYRFATIDGQSVKAMINDDAWN
                     KDTFLPTVGKRGAAIIAARGVSSAASAANAAIDHMRDWALGTNGQWVTMGIPSNGEYG
                     IPKGVMFGYPVTTAGGEYKIVEGLEIDAFSQERINITLKELTDEQDGVKHLL"
     gene            3438..4076
                     /locus_tag="PKV56_17260"
     CDS             3438..4076
                     /locus_tag="PKV56_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69605.1"
                     /translation="MTALASISTPSCPARAAASGLIPCASAAAVALCIALGGMVSLPA
                     LAAEPAAAKSADKVVPRASSQTKAKAGSKKALPIDQPPEPASDEQRSAAERVYYGEYQ
                     CEFKQVVHTKPSEQHPAYVDLRFAKSTYLMKPVLSSTGAIRLEDIKGQTLMVQIASKS
                     MLLNVKAGNRLVDECVGEGHRAELLAAKERIALEAEPPSLGSSVSASAKPTP"
     gene            4193..>5672
                     /locus_tag="PKV56_17265"
     CDS             4193..>5672
                     /locus_tag="PKV56_17265"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848455.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HOX69606.1"
                     /translation="MLEAYRLHVAERAALGIPPLPLSAKQTSELIELLKTPPAGEEKT
                     LVDLITHRVPAGVDDAARVKASYLAAVAHGTEKCSLISPETATQLLGTMLGGYNISPL
                     IDLLDSPAVGSVAAAGLKNTLLMFDQFHDVKDKADKGNANAKSVLQSWADAEWFTSRP
                     EVPKSITVAIFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNARPGITPEEDGKRG
                     PIRFIEDLRAKGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGA
                     KIAPIFYNTMEDAGALPIELDVNQMNMGDVVELRPYEGKALKDGKVIAEFKVKSDVLF
                     DEVRAGGRIPLIIGRGLTAKAREALGLPVSTLFRLPQNPADTKKGYSLGQKMVGKACG
                     LPIVNGQQVGVRPGTYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHT
                     AAYPKPVDVKMHHELPDFISHRGGIALRPGDGVIHSWLNRLLL"
BASE COUNT         1173 a         1868 c         1691 g          940 t
ORIGIN      
        1 acgaccttgg gcgcctcgcg gcacatgaac tcgatcgcgt cctggtcacc cagccagtcc
       61 gaacccttca cggtgtcata gaagtggtag tgccagttgt cctcggacat gttgcccaga
      121 ctcgcgccga tgccaccctg cgcggcgacc gtatgcgatc gcgtgggaaa cactttcgac
      181 agcaccgcga cattcagacc ggcacgagcc agctgaagcg atgcccgcat gccggaacca
      241 ccagcaccaa cgatcacgac atcgaacttc cgcttcggaa cagaagaact caacgaactc
      301 attgttgacg aacccatttg cattacagcc tccacagcac ttggattgcc caacccgcac
      361 agcccaccag ccagacgatg gtgaacacct gcaaagccag gcgtatgccg accggcttga
      421 cgtaatccat ccagacgtca cgcatgccca cccacacgtg gtagagcaac gaaacgatca
      481 cgacgaaggt gagcaccttc atccactgct gggaaaagat gcccgcccac ttgtcgtagc
      541 ccatctcgcc gggcagcaag atctggacga tgaccgccag cgtgaacagg gccatcacgg
      601 cggccgttac ccgctgcgac aaccagtcac gcagcccgta atgcgcaccg acgacgatgc
      661 gcttcgatcc gaaattctgt gccatgaggt ccgattcaga agaaaagctt ggaaccgagc
      721 agagccgtca gcagcaggct caaaccgagg gtcgcgagcg ccgagctgtg accgaactcc
      781 ttggtcactg cgtgcgtcat gtccatccac agatggcgga ccccggcgat gaagtggtgg
      841 agaaacgccc aggtcaggcc cagcgcgatc agcttgaaga cccatcccgg caggatgccc
      901 aagcctcccg tgaagacgct ggtgaactgg ctgtaggaga tctcggaagt gacgctggtg
      961 tcgaacatcc agatgatgaa cggcatcagc aggaacatgc cgacaccgct gacgcggtgc
     1021 aggatcgaca cgatgccggc aggcggcaaa cggtacttga cgatctggct gatgtgaata
     1081 ttgcggtgag tgggccgcgg cttcagggtt tctgacatct acatctttcg tttcgcagcg
     1141 atctgcaaac cactgagttt attgcaatgc agcacgtgct aataccgaca tgagtcgaaa
     1201 cgcatgaagg cctgtgcacg ggcgcaatcg agaggggaat tcagctgagc gcattccggt
     1261 agtggtaacg cgacgtgttg tagaggccgc gacgcaactc gaccggcttg tcgtcgtagg
     1321 tcaaggacag gcgctcgacg ctgagcagcg gaaagcccac gtcgaccgcg agcaactgcg
     1381 cagacgcggg atccgccgtc accgcacgga tcttctcgtc ggcgcggatc atgtgaaccc
     1441 cgaactcgct ttcgaagagc ccgtacatcg ggccccggta atcgctcaga cgctctgcgg
     1501 tcaggccctt gaagaggagt ccaggcaacc agatgtcgtc gagcacgacc ggcgtggagt
     1561 tgaacgtgag aacccgcctg acgttgacga ccgaatcgcc cgggctcatc gcgagcgcac
     1621 gtgccacatc ggcagtcgat cgaagacgtc ggcaatcgat gaaacgccgc tggacggtac
     1681 cggattcgcc gtcgtccggc atcaggcgca ggaaccggta ctgcaccttc tgctcggcat
     1741 gcgtggcaac gaaagtgccc ttgccctgcc gccgcacgag caggttttca gccgccagtt
     1801 cgtcgatggc cttgcgcacc gtgccctggc tgaccttgaa gcgcgcggca agctccattt
     1861 cgctcgggat ggcctcgccc ggacgccact cgcccatacg caggctgcgc gtcatcagcg
     1921 tcttgatctg ctggtaaagc ggactgaagg acggagaggt gccatcgccc gggcgagccg
     1981 ccggcagcgg ctccacagca agtgcggacg aattcttcat ggcgcgaatt gcagcacagg
     2041 agtgaggtga tagtccagcc gaaactggtg atgtttgact gacatcttat gtcttacata
     2101 agacttttga cttgccccgg gttcatccct acacttcaaa gtcccgctgc atacaattag
     2161 gggtttgctc ggttgccgtc cagaccgcgc ctccgcttat ccgtcccgat tccgcactca
     2221 accttttctg gagattccac atgagcaagt cccccgttcg cgttgccgtc accggcgctg
     2281 caggccaaat cggttacgca ctgctgtttc gcatcgcctc tggagaaatg ctcggcaagg
     2341 accagccggt catcctgcaa ctgctcgaga tcccggacga aaaggcccag aacgcgctca
     2401 agggcgtgat catggaactc gacgactgcg cgttcccatt gctggctggc atcgaagccc
     2461 acagcgaccc ggtcgcggca ttcaagaaca ccgactacgc gctgctcgtg ggtgcccgcc
     2521 cacgcggccc cggcatggag cgctccgatc tgctggccgc caacgcccag atcttcactg
     2581 cccagggcaa ggcgctgaat gcgtctgcct cgcgcaacgt caaggtgctg gtggtcggca
     2641 accctgccaa caccaatgcc tacatcgcta tgaagagcgc gcccgatctg cccgcgaaga
     2701 acttcaccgc catgctgcgc ctcgatcaca accgcgctgc gtcgcagatc gccgcgaaga
     2761 ccggcaagcc ggtgtcgagc atccagaagc tggccgtgtg gggcaaccac tcgccgacca
     2821 tgtatgccga ctaccgcttc gccacgatcg acggccaatc ggtcaaggcc atgatcaacg
     2881 atgacgcgtg gaacaaggac acgttcctgc cgaccgtagg caagcgcggc gccgccatca
     2941 tcgccgcgcg cggcgtgtcg tctgcagcgt cggccgccaa cgccgccatc gaccacatgc
     3001 gcgactgggc gctcggcacc aacggccagt gggtgacgat gggcattcca tctaatggcg
     3061 agtacggcat ccccaagggc gtcatgttcg gctatccggt caccaccgcg ggcggcgagt
     3121 acaagatcgt cgaaggtctg gaaatcgacg ccttcagcca ggaacgcatc aacatcacgc
     3181 tcaaggaact gaccgacgag caggacggcg tcaagcacct gctctgatcc cggacccaca
     3241 cagctgcgtc atgcgcccag cgcccaagcc ggccacccct gcggaggccg gctttttttc
     3301 ggctgttcgg gtggcttcgg cccggaacct gcaacagcgt tcggcgcaac cagccgtttc
     3361 gaatttctgc gcctcccgcg ctcgatgtgc accgagcttg tgctgaaaat ccctggtccg
     3421 aaccaccggt gacgtcaatg accgctctcg catctatttc gactccatcg tgcccggccc
     3481 gcgctgcggc gagcgggctg attccatgcg catcggcggc cgctgtagcg ctttgcatcg
     3541 cactgggcgg gatggtgtcg ctgcctgcgc tcgcggctga gcctgccgcg gccaagtcgg
     3601 ccgacaaggt tgtgccgaga gccagctccc aaaccaaggc caaggcgggt tcgaagaagg
     3661 cactgcccat cgatcagccg cccgaacctg caagcgacga gcaacgcagc gctgcggaac
     3721 gcgtctacta cggtgagtac cagtgcgagt tcaagcaggt cgtccacacc aagccaagcg
     3781 aacagcatcc ggcctatgtc gacctgcgat tcgccaagtc gacctacctg atgaagcccg
     3841 tgctgtcatc cactggcgcg atccggctcg aggacatcaa ggggcagacc ctgatggtgc
     3901 agattgccag caagtcgatg ctgctcaacg tgaaggccgg gaaccgactg gtcgacgaat
     3961 gcgtgggaga gggccatcga gcggagttgc tggcggccaa ggagcgcatc gcgctggagg
     4021 cagagccgcc gtcactggga agttccgtca gcgcctcagc gaagccgacg ccctgaactg
     4081 cgaatccgcg cacatcgacc ccaacaccaa accccagaaa atgttcgctg cctccggctt
     4141 gaccgagcag aacgaacgtt cgaattccag tcatctatga aggagccaca ccatgcttga
     4201 agcctatcgc ctccatgtcg ccgaacgcgc cgccctgggg attccgccgc tgccactgtc
     4261 ggccaaacag acgagcgagt tgatcgagtt gctgaagacc cccccggctg gtgaagagaa
     4321 gacgctggtc gatctcatca cgcaccgcgt gcctgccggc gtggacgacg cagcccgcgt
     4381 caaggccagc tacctggcgg ctgtcgctca cggcaccgag aagtgttcgc tgatcagccc
     4441 cgagaccgca acccagttgc tcggcacgat gctcggcggc tacaacatca gcccgctgat
     4501 cgacctgctc gacagccctg cggtcggctc cgttgccgct gccggcctga agaacacgct
     4561 gctgatgttc gaccagtttc atgacgtcaa ggacaaggca gacaagggca atgccaacgc
     4621 caagtcggtg ctgcagagct gggcggacgc ggaatggttc acctcgcggc ccgaagtgcc
     4681 caagagcatc accgtggcga tcttcaaggt caccggcgaa accaacaccg acgacctgtc
     4741 acccgcaccg gacgcctgga gtcgccccga catcccactg catgcgctgg cgatgctgaa
     4801 gaacgcgcgg cccggcatca cgcccgaaga agacggcaag cgcggcccga tccgtttcat
     4861 cgaagacctg cgcgccaagg gcaacctggt ggcctacgtc ggcgacgtgg tcggcaccgg
     4921 ctcgagccgc aagtcggcca ccaactcggt gctgtggttc accggcgaag acattccgtt
     4981 cgtgccgaac aagcgcttcg gcggtgtgtg cctcggcgcc aagatcgcac cgatcttcta
     5041 caacacgatg gaagacgccg gcgccctacc gatcgagctc gacgtgaacc agatgaacat
     5101 gggcgacgtg gtcgagttgc gcccctacga aggcaaggcg ctgaaggacg gcaaggtcat
     5161 cgccgagttc aaggtcaaga gtgatgtgct gttcgacgaa gtgcgtgccg gtggccgcat
     5221 cccgttgatc atcggccgag ggctgactgc caaggctcgc gaagcgctgg gcctgcccgt
     5281 cagcacgctg ttccgtctgc cgcagaaccc ggccgacacc aagaagggct acagcctcgg
     5341 ccagaagatg gtcggcaagg cctgcggcct gcccatcgtc aacggccagc aggtcggtgt
     5401 gcgcccgggc acgtattgcg agccacgcat gacctcggtc ggttcgcagg acaccaccgg
     5461 cccgatgact cgcgacgagc tcaaggacct ggcctgcctg ggcttcagcg ccgacctggt
     5521 gatgcagtcg ttctgccaca ccgctgcgta tccgaagccg gtcgacgtga agatgcacca
     5581 cgaactgccc gacttcatca gccaccgcgg tggtatcgcg ctgcgcccgg gcgacggcgt
     5641 gatccacagc tggctcaacc gcctgctgct gc
//
LOCUS       DAOORV010000211         5605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17477_length_5605_cov_9.658559, whole genome shotgun sequence.
ACCESSION   DAOORV010000211 DAOORV010000000
VERSION     DAOORV010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17477_length_5605_cov_9.658559"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKV56_17270"
     CDS             <1..242
                     /locus_tag="PKV56_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOX69607.1"
                     /translation="IVLSAGFAALLAWGWGLHPATALLATSPGGMAEMAITASVLGLG
                     VPVVTAFHVVRYAAVLVLTGPLWRLEAARLARRPG"
     gene            610..1044
                     /locus_tag="PKV56_17275"
     CDS             610..1044
                     /locus_tag="PKV56_17275"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HOX69608.1"
                     /translation="MASIRSRFALFALVLFASLPAQAQSTTEPAAALQGMDVVSYFKE
                     GGPLKGDAAFRHDFDGARYLFSSAQNKATFVADPDRYLPQFSGLCATGIALGKEFKGD
                     PNTWKIVDGKLYLYHSGPVRAKGDADPTTIAKAHQNWAARKK"
     gene            complement(1051..2883)
                     /locus_tag="PKV56_17280"
     CDS             complement(1051..2883)
                     /locus_tag="PKV56_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX69609.1"
                     /translation="MTYGPSDDAPQGTNRRSDNTGTAASAWQVGAWRIDAVTGELRRS
                     GETVRLEPKVAEVLVHLARRAGEVVSRDELLTAVWPGVVVGDDALTQVIIKLRKALGD
                     DAKRPAYIETLSKRGYRLIAPVARAADAAAAVSAAATAGTLAAATAAPPPPAEVAERT
                     ERPAASALLRSKPRPWRLALLGGGLTALLVATGWGYGIYRGWAWPIGSENTPRAAAEA
                     LPVIAVLPLVNASGDPAREFFSDGVSDEIIHALGRYSGLRVISRSSVQQFKQREASTS
                     VVSKELGARYVVTGAVREADGQVRVAVELSDAETARVLWSERFEGQGGEVFKIQDSIV
                     QNIVGALAVKVSMLESDRAATRAPSRREAYDLVLLARAQLLKADRAANRQGRALIVQA
                     RELAPDYAMAYVVESELEDQRANLGWVEDPAQGLLLSERATRHALSLNDPGVNARAHA
                     QLGRIHVIRGEFSQALAESERARALNPSDPFIAEVRADTLLWAGRTQEAVELMESAIR
                     LDPAARRSPVRQAIAIAYFASERYPEALAASERALADYPETVAMHTMRAAVLAQMGRL
                     EEARQSVAEVRRLQPNFPVAQFGNRFADPVISERFQAALSKAGF"
     gene            2998..4449
                     /locus_tag="PKV56_17285"
     CDS             2998..4449
                     /locus_tag="PKV56_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69610.1"
                     /translation="MSLFKFHALATAVMVPQFPAESTRLDDPLLLPDEGTSLSRCSLV
                     HSNPFFSHQGDDMKTQAKPFRRLARLLLVPIVAGMAACATPQGGAPAVTVTATQSNVV
                     SFWNDIANKTVLAPTPASAANPTPEEQRPTFFFDVASVHVAIYDAVVAIEGRYKPFAV
                     KPKSPAAGASTEAAVNAAAYGVLKALFPNRAAVYQAAYEQRLAGLPDGNAKTMGVALG
                     SEVAAEVLRLRANDGRMVALAPYVSGTAPGEFRAANPNPVFRHFPSIKPFALQSLSQF
                     RPAPPPALNSAAYAAAVDQTRALGGTASTARTPEQLEVARFHTEPPPPAITRNLGRFA
                     SSTANVGDAARLMAMIYVSYVDAIGACFEAKYHYQTWRPVSAIQGADAAWTPVLPTPN
                     HPEYPAAHSCTSGSVGEALRHFYGTPNVTFTWDSKVTNTTRSYAGIDAFNAEAGLARI
                     HGGMHFSHATAAGEELGKRVAQWVASRHFGRQD"
     gene            4500..5306
                     /locus_tag="PKV56_17290"
     CDS             4500..5306
                     /locus_tag="PKV56_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69611.1"
                     /translation="MNTNRRHVLQLAALSTTALALPGRALATVTERSGSPDRKVTHTT
                     MWRNTLSCDNAGVPVSQRNGRLATPADFGSAQQCTMLKDSIEGPFYFCTNPGVADIAR
                     GQPGAPLVIALRAINAATCEPIPDAVIDIWHCDARGLYSGQSMAVDEAIKSPKHTPPV
                     NDDRHCRGALRTDADGIAEFRSIYPGYYIERAVHVHFKVHIGQRAFLTREALLPEADN
                     VAVHAMVPYNVPRKGPRIRNAQEADWGNPTMKIVERPQTRLALLDVVLSV"
BASE COUNT          900 a         2016 c         1806 g          883 t
ORIGIN      
        1 tgatcgtgct gtcggccggc tttgccgcgc tgctggcctg gggctggggg ctgcatccgg
       61 ccaccgcgct gctggccacg tcccccggtg gcatggccga gatggccatc accgccagcg
      121 tgctggggct gggcgtgcct gtggtcaccg ccttccatgt ggtgcgctat gcggcggtgc
      181 tggtgctgac cgggccgctg tggcggctgg aagcggcgcg gctggcgcgt cggcccgggt
      241 agggtggtgt gctcgaattg ttgatgccag tcgacgccat gtgatgtcgg gttcatcatt
      301 taccggcctc acacttccgg ccaggagcag cccgtcgcga caggctgctt cagttcgcca
      361 cacattcgtc aacgtgtccg ctgcgagacc taccgcctcg gcggttcgca aagcagacac
      421 ttgtccccaa cggggaatgc ggtgcccacc caagcggaag gcgggttgtt gaaggtcagg
      481 cggcgcgcct ctcctcgaaa gccgcgaact cgtcacgcgt ggacgttggt tccgattgac
      541 acacccccgg cgccgctcga acatccgcta cagctgaacc ctattgttca atcaccaagg
      601 agttctgaca tggcaagcat tcgcagtcgc tttgcgttgt tcgcactggt gctgttcgcc
      661 agcctgccgg cacaggcaca aagcaccacc gagcccgcag cagcgctcca gggtatggac
      721 gtcgtcagct acttcaagga aggcggcccg ttgaaaggtg atgccgcgtt ccgccacgac
      781 ttcgatggcg cgcgctacct gttctcgagc gcacagaaca aggcgacctt tgtcgccgac
      841 cccgaccgct acctgcccca gttcagcggc ctttgcgcca ccggcatcgc cctgggcaag
      901 gagttcaagg gcgacccgaa cacatggaag atcgtggacg gcaagctcta cctctatcac
      961 tcgggccccg tgcgcgccaa gggcgacgcc gatcccacca ccatcgccaa ggcacatcag
     1021 aactgggcag cgcgcaagaa gtaagcagcc tcaaaacccc gccttgctga gcgcggcctg
     1081 gaagcgctcg ctgatcaccg ggtctgcgaa gcggttgccg aactgcgcca ccgggaagtt
     1141 gggctgcagc cgcctcactt ccgccaccga ctggcgcgcc tcttcgagcc ggcccatctg
     1201 cgccaggacc gctgcacgca tggtgtgcat ggccactgtc tccgggtagt cggccaacgc
     1261 acgttcgctt gcggccaggg cctccgggta gcgttcagac gcgaagtacg cgatcgcgat
     1321 cgcttgtcgc accggcgagc ggcgcgcggc cgggtcgagg cggatcgcgc tctccatcaa
     1381 ctccaccgcc tcctgggtgc gccctgccca aagcagcgtg tcggcgcgca cctcggcaat
     1441 gaaggggtcg ctggggttga gggctctggc acgttccgat tcggccagcg cttgagaaaa
     1501 ctctccacgg atgacatgga tacggccgag ctgggcatgg gcacgggcgt tgacgcccgg
     1561 gtcgttgagt gacagggcat gccgggtcgc tcgttcggag agcagcaggc cctgcgccgg
     1621 atcttccacc cagccgagat tggcgcgctg gtcctcgagc tcgctttcga cgacataggc
     1681 catggcgtag tcgggtgcca gctcgcgggc ctggacaatc agggcgcggc cctggcggtt
     1741 ggcggcgcgg tcggccttga gcagttgggc acgcgccagc agcaccaggt cgtaagcctc
     1801 gcgccggctg ggtgctcgcg tggcggcgcg gtcgctttcg agcatgctca ccttgacggc
     1861 cagcgcgccg actatgttct gcacgatgct gtcctggatc ttgaacactt cgccgccctg
     1921 accctcgaag cgttctgacc acagcacccg cgcggtttcg gcatcgctca gttccaccgc
     1981 cactcgcacc tggccgtcgg cctcgcgcac ggcgccggtg accacatatc gcgccccgag
     2041 ttccttgctc acgacgctgg tcgacgcttc gcgctgcttg aactgctgca ccgaactgcg
     2101 cgagatcacg cgcaggcccg agtagcgacc gagcgcatgg atgatctcgt cgctgacgcc
     2161 gtcgctgaag aactcgcgcg ccggatcgcc gctcgcgttg accagcggca gcacggcgat
     2221 caccggcagg gcttcggcgg cggcgcgcgg cgtgttctcg ctgcctatcg gccaggccca
     2281 gccacggtag atcccgtagc cccaacccgt tgcgaccagg agcgcggtca agccgccacc
     2341 cagcagcgcc aatcgccagg gccgcggttt cgaccgcaac agcgctgacg ctgccggcct
     2401 ttcggtgcgt tcagcgacct cggcgggcgg cggtggtgcc gcggtcgcag ccgccaaggt
     2461 gcccgccgtc gctgcggcag acaccgcggc cgcggcatcg gccgcacgcg ccacgggcgc
     2521 gatcagcctg tagccgcgct tggacagggt ttctatgtag gccggccgct tggcgtcgtc
     2581 gcccagcgcc ttgcgcagct tgatgatgac ctgggtgagg gcgtcgtcgc cgaccaccac
     2641 gccgggccac acggcggtca gcagttcgtc gcggctgacc acttcgccgg cgcgccgggc
     2701 cagatggacc agcacttcgg ccaccttggg ttcgaggcgc accgtctcgc cgctgcgacg
     2761 cagttcgccg gtgactgcgt cgatccgcca agcacccacc tgccacgccg aagcggctgt
     2821 gccggtgttg tccgagcgcc ggttcgtgcc ctgcggggcg tcatcggacg gtccgtaagt
     2881 cattgatcga aagggcgaat tcttcgttag ccgtttgttc ggcgtttgct agccgagcca
     2941 tcgggacttg cgcgaggcga tcgattgtca tcgctcctgt gcttcacgga ggtccccatg
     3001 agcctgttca agttccacgc cctcgccacc gcagtgatgg tgccccagtt cccggcggag
     3061 tccacccgcc tggatgaccc gctgctgctg cccgatgagg gcaccagcct gtcccgctgt
     3121 tccctagttc attccaaccc attcttttca caccaaggag atgacatgaa gacccaagcc
     3181 aagccgttcc gtcgccttgc ccgcctgctg ctcgttccca tcgtggccgg catggcagcc
     3241 tgcgccacgc cgcaaggcgg tgcgccggcc gtcaccgtca cggcgaccca atccaacgtg
     3301 gtgtccttct ggaacgacat cgccaacaag acggtgctgg cgcccacgcc cgcatcggcg
     3361 gcgaacccca cgcccgaaga gcaacggccc acgttcttct tcgacgtggc cagcgtgcat
     3421 gtggccatct acgacgccgt ggtggccatc gaggggcgct acaagccgtt tgcggtgaag
     3481 ccgaagtcgc cggccgccgg cgcgtcgacg gaagccgcgg tcaacgccgc cgcgtacggt
     3541 gtgctgaagg cgttgttccc gaatcgcgct gcggtgtacc aggccgccta cgagcagcgc
     3601 ctggccggcc tgcctgatgg caacgcaaag acgatgggcg tggctctggg cagcgaggtg
     3661 gccgccgagg tgctgcgcct gcgcgccaac gacgggcgca tggtggcgct cgcgccctat
     3721 gtctccggca cggccccggg cgagttccgc gccgccaacc cgaacccggt gttccggcac
     3781 ttcccgtcga tcaagccgtt cgcgctgcaa agcctgagcc agttcaggcc ggcaccgccc
     3841 ccggccttga acagcgccgc ctacgccgcc gcagtcgacc agacacgcgc gctcggcggc
     3901 acagccagca ccgcacgcac gcccgaacag ctggaggtgg cgcgcttcca caccgaacca
     3961 ccgccgccgg ccatcacgcg caacctcggc cgctttgcgt ccagcacggc gaacgtgggg
     4021 gacgcggccc ggttgatggc gatgatctac gtcagctacg tcgatgccat cggcgcctgc
     4081 ttcgaggcca agtaccacta ccagacctgg cgcccggtga gcgcgatcca aggggccgat
     4141 gccgcctgga cgccggtgct gcccacgccc aaccaccccg agtaccccgc tgcccactcc
     4201 tgcacctcgg gcagcgtggg cgaagccctg cgccatttct acggcacgcc caacgtcacc
     4261 ttcacctggg acagcaaggt caccaacacc acgcgcagct acgccggcat cgacgcgttc
     4321 aacgccgaag ccggcctggc gcgcatccac ggcggcatgc acttcagcca tgccaccgcc
     4381 gccggcgaag agcttggcaa gcgggtggcg cagtgggtgg catcgcgcca cttcgggcgc
     4441 caggactgaa gcgcaatagc cacctcatcc acccatcctt accgcaagga gaacgcctca
     4501 tgaacacgaa ccgccgccac gtcctgcaac tggccgcctt gtccaccacc gcgttggcgc
     4561 tgccaggccg cgccctggcc accgtcaccg aacgctccgg ctcgccggac cgcaaggtca
     4621 cccacaccac catgtggcgc aacacgctga gctgcgacaa cgccggcgtg ccggtgtcgc
     4681 aacgcaacgg ccgcctggcc acaccggccg atttcggcag cgcgcagcaa tgcacgatgc
     4741 tgaaggactc gatcgaaggc ccgttctact tctgcaccaa cccgggcgtg gccgacatcg
     4801 cgcgcggcca gcccggcgcg ccgctggtga tcgccctgcg cgcgatcaac gccgccacct
     4861 gcgagcccat ccccgacgcg gtgatcgaca tctggcactg cgatgccagg ggcctgtact
     4921 cggggcagag catggcggtc gatgaagcca tcaagagtcc gaagcacacg ccaccggtca
     4981 acgacgaccg gcactgccgc ggcgcgctgc gcaccgatgc ggacggcatt gccgagttcc
     5041 gcagcatcta cccgggctac tacatcgaac gcgccgtcca tgtccacttc aaggtacaca
     5101 tcggccagcg cgcctttctt acgcgagagg cgttgcttcc ggaagccgac aacgtcgccg
     5161 tgcatgcgat ggtgccctac aacgtgccgc gcaagggccc gcgcatccgt aacgcacagg
     5221 aggcggactg gggcaatccg acaatgaaga tcgtcgagcg gccgcagacg cggctggcgc
     5281 tgctggacgt ggtgctgtcg gtctgacgtc cgggcccgag caagcgggcc gcgacgagac
     5341 ctccgtgact gccgcccgag ccgtcctcga ggccctgcat cgcgttcggg ccaggcgctc
     5401 tccagactgc gaagccaggt gggccggcct cgccgatcac cgctgcgcgt gttgatggac
     5461 agggacagaa ccatgcggca caccacatca gcgactgcag cgccgggcca gcgccggctc
     5521 agcgccaggt cgcccggccc cgctggtgct tggccaggaa cagctcgcgc aggttcacct
     5581 cgcgcgggcc gggcaccagg gtgag
//
LOCUS       DAOORV010000212         5574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17587_length_5574_cov_7.910129, whole genome shotgun sequence.
ACCESSION   DAOORV010000212 DAOORV010000000
VERSION     DAOORV010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17587_length_5574_cov_7.910129"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..370
                     /locus_tag="PKV56_17295"
     CDS             152..370
                     /locus_tag="PKV56_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69612.1"
                     /translation="MSITYKVEFKNDPACSNSGALAAAAGGCVRTSIPRYDGERDMAM
                     IEVPDDAAAQLLEKLAGADGNVLTFKVR"
     gene            598..2355
                     /locus_tag="PKV56_17300"
     CDS             598..2355
                     /locus_tag="PKV56_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX69613.1"
                     /translation="MGLSGSMGITKRLWIVAAVLSLSLAALAGFAYFKLNRVVAYAQS
                     AEHSRVPQLAEAGAMQLNITRISLQLRHAMLSRTPAEREASLADIAAKRKLVNDSAGL
                     YEKALFTEAGKAMFAPLQPRLEKLWQNADANVALIQSGKKDDAFAYLVDHTIPARNDV
                     LAVLDGMVKYQRDQLSADVELIAVDIRSTLASLVTLVVFSIVGLLLSSAYVARVLHRR
                     IALSQGVAERARDGDLMTPIEDDARDEFSPLLSALREMQGALAAIVSSVRGNAESVAT
                     ASAEIAQGNLDLSQRTEQQASALEETAASMEQLGSTVKQNADNARQASQLAQSASEVA
                     GQGGDVVRQVVDTMKDINDSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVASEVRSLAHRSAAAALEIKSLITTSVERVEHGSTLVDRAGSTMLDVVTSIKRVT
                     DIMAEISAASIEQSSGVSQVGDAVSQMDQATQQNAALVEESAAAAESLKMQARKLVEA
                     VAIFKLTQSDAFVRPVHPVVVATAPKVDRRGPDRAKNVSRLTVKARPLASPSPAPAAA
                     VPRVVSAAAKTGTDDWETF"
     gene            2399..2932
                     /locus_tag="PKV56_17305"
     CDS             2399..2932
                     /locus_tag="PKV56_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX69614.1"
                     /translation="MSTLIEERPAVQQATAPATAAPAMPSEFLTFRLGGEEYGIDILR
                     VQEIRSYEAPTRIANAPAFVKGVVNLRGVIVPIVDMRLKLGCDTAEYREFTAVIVLNV
                     RGRVIGAVVDSVSDVLALGADQIKAAPELNSSIDAGYIMGIGCVKSGDVERMLILTDI
                     DALMSSPEMGLINAAAY"
     gene            3227..3886
                     /locus_tag="PKV56_17310"
     CDS             3227..3886
                     /locus_tag="PKV56_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69615.1"
                     /translation="MLKRLLPLLTLLPALAPAAQVVFTNSTDVTTYSMVTVDSRKLKT
                     GLAAGLVAYPGEECIAFTDTNVVPVCGGFTISNGWMYPNAGGFPGWPDPNDPRKDLRI
                     DGLKTKDITNFLSPGQCTAAGCPAAPPPVPLTLTFTRPTVEFGMMFRASWETQQTPFT
                     TGFRVIANGVDLGTYPVPVQGVQTLGVFAPEGLKTVTIVPYNIDPTVVGPTVIHRIYT
                     K"
     gene            complement(3917..5311)
                     /locus_tag="PKV56_17315"
     CDS             complement(3917..5311)
                     /locus_tag="PKV56_17315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX69616.1"
                     /translation="MRAPTATPAATNYYDGLNLKLLDAIPAKARRVLELGCANGLLGR
                     RFKELHPGVQWCGVDVSADAVAAASQHLDRAVQLDLDNADLSVLEGGFDVIVIGDLLE
                     HLRDPGRVLSALYDLGTEDARIACCLPNMGHLSVIERLVAGDISYDAAGLLDRTHTRF
                     YTPSSAFKTFLDSGWMPHLKDQYRFKLPQTHFAARIVDAAVALGLPMETAVHNLGLYQ
                     MILVCEKWPLQSLLRPGPRVPFSVIVPVNRPWQHELNVARSPGLQEIDAEVICVQGAS
                     SAAAAYVSGSQRATHAWRVMAHQDVYFPTGSGYALAQQLGAVEQAGLIGAPIGFAGLE
                     PDPARKGTVRKAGLVIDRTTLFRHQPSAGAVSMDEFAVALHRDSDVSIDPALGWHLWA
                     TDLCLQAEQKAKRPIAQILDVPLFHNSTNAHQLPPEFHSSARVLMDKYPTRQRFATLC
                     GELTRKNAAALHAA"
     gene            complement(5332..>5574)
                     /locus_tag="PKV56_17320"
     CDS             complement(5332..>5574)
                     /locus_tag="PKV56_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69617.1"
                     /translation="APERRPVVPVYSAPAAGSPAPMKPMPSILPAAAAPVGAPTLPAA
                     RPAATAPGGLDIATLDLLATTRSEALRRAAQAAVAG"
BASE COUNT          962 a         1805 c         1907 g          900 t
ORIGIN      
        1 cgtggtcgca cggtggcggt gggatactgc cccgcgcggg agccctccac cgagatcgca
       61 cgagcggtgc ggcatcgacg agcggacaga ccgcgcagca gacctccggg atgcttggca
      121 gacaacgaat acaacaacga ggcaaacagt aatgagcatc acctacaaag tcgaattcaa
      181 gaatgaccct gcatgttcca acagcggggc cctggcggct gctgccggtg gttgcgtgcg
      241 cacgagcatc ccgcgctacg acggagagcg cgacatggcc atgatcgagg tgcctgacga
      301 cgcggccgcg cagctgctgg agaagctggc aggtgcagac ggcaacgtgc tgaccttcaa
      361 ggttcgctga cagtcggccg cgggccctcc gcggccgctg tcgcggctca ggtcgcaata
      421 agagcaaacg gctccatcgg gggccgtttt tttttgcttc agacgcaatg aaggacatgc
      481 ccggagagcg caggctgcgc tcccccggaa gcttcgaatc cggcatcaag ttcgagccgg
      541 atcagccgaa tacacgagca tcgagcgttt cgttttgttc atattcaggg ggaagtcatg
      601 ggtctgtcgg gttcaatggg aatcacgaag cggttgtgga tcgtcgccgc cgtgctgtcg
      661 ctgtcgctgg ctgccctggc ggggtttgcc tatttcaagc tgaacagggt ggttgcttac
      721 gcccagtccg ccgagcattc gcgagtgccg caactggccg aagcgggtgc gatgcaactc
      781 aacatcaccc ggatctcgct gcagctgcga catgccatgc tgtcgcgcac acccgccgaa
      841 cgggaggcct cgctggcgga catcgcggcc aagcgcaagc tggtcaatga ctcggcaggc
      901 ctgtacgaga aggccctctt caccgaggcc ggcaaggcca tgttcgcgcc gctgcagccc
      961 cgactcgaaa agctctggca gaacgccgat gcgaacgtgg cgctcatcca gtccggaaag
     1021 aaggacgacg cgttcgccta cctggtggac cacacgatcc cggcccgaaa cgacgtgctg
     1081 gcggtactcg acgggatggt caagtaccag cgcgaccagc tcagcgccga cgtcgaactg
     1141 atcgccgtgg acatccgcag cacgctggcg agcctggtca cgctggtcgt cttctccatc
     1201 gtcggcctgc tgttgtcgtc agcctacgtc gcgcgcgtgc tccatcggcg cattgccctt
     1261 tcacagggcg tggccgagcg ggcgcgggat ggcgacctga tgacgccgat cgaggacgac
     1321 gcgcgtgacg agttctcgcc tctgctgagt gcgttgcgcg agatgcaggg tgcgttggct
     1381 gccatcgtgt ccagcgtgcg cggcaacgca gagagcgtgg ccaccgccag tgccgagatc
     1441 gcccagggca acctggacct gagccagcgt accgagcagc aggccagcgc gctcgaggaa
     1501 accgctgcat cgatggagca gctcgggtcg actgtcaagc agaacgccga caacgcccgc
     1561 caggcgagcc agcttgccca gagcgccagc gaggtcgcgg gccagggcgg cgacgtcgtg
     1621 agacaggtcg tggacacgat gaaggacatc aacgacagca gcaggaagat cgtcgacatc
     1681 atcggagtca tcgacgggat tgccttccag accaacatcc tggcgctgaa cgcggctgtg
     1741 gaagccgccc gtgccggaga gcagggtcgc ggcttcgcgg tggtggccag cgaggtgcgc
     1801 agcctggccc atcgcagtgc ggcggcggcc ctggagatca agagtctcat caccaccagc
     1861 gtggagcgtg tcgaacacgg cagcacgctg gtcgaccggg ccggcagcac gatgctggac
     1921 gtcgtgacat cgatcaagcg tgtcaccgac atcatggccg agatcagtgc cgccagcatc
     1981 gagcagagtt caggcgtctc gcaggtcggc gatgcagtca gccagatgga tcaggccacc
     2041 cagcagaacg cggcactggt cgaggaaagt gccgctgctg cggagagcct caagatgcag
     2101 gcgcgcaagc tggtggaagc tgtcgcgatc ttcaagctga cccagtccga cgcgttcgtg
     2161 cggccggttc accccgttgt ggtcgcgact gcgccgaagg tcgatcgccg cggaccggac
     2221 cgggcgaaga acgtgagccg cctgaccgtc aaggcccggc cgcttgcgtc accgtcaccg
     2281 gcacctgcgg ctgcagtgcc ccgcgtcgtc agcgcagccg ccaagaccgg caccgacgac
     2341 tgggaaacgt tctgaacccg cccagccgaa ccccttttct tcttgtcagg agacccacat
     2401 gagcacattg attgaagagc gccccgcggt gcagcaagcc acggcacctg ccactgccgc
     2461 ccccgccatg cccagcgaat tcctcacctt ccgtctgggc ggcgaggagt acggcatcga
     2521 catcctgcga gtgcaggaga tacggtccta cgaagcaccc acgcgcatcg ccaatgcgcc
     2581 tgccttcgtg aagggcgttg tcaacctgcg cggcgtcatc gtgcccatcg tcgacatgcg
     2641 cctgaagctc gggtgcgata ccgccgagta cagggagttc acggcagtca tcgtcctgaa
     2701 tgtgcgcggt cgcgtgatcg gcgcggtggt cgattcggtg tccgacgtgc tggcgctggg
     2761 cgccgaccag atcaaggcgg ctcccgagct gaactcgtcg atcgatgccg gttacatcat
     2821 gggcatcggt tgcgtgaaga gcggggatgt cgagcgcatg ctcatcctca ccgacatcga
     2881 cgcgctgatg agcagtcctg agatggggtt gatcaacgct gccgcttact gatcacccgg
     2941 gagcgcacgc gaacctgtgg cccgccgggg gccgatgcgc cgagcgtgca gtcagatccg
     3001 gaccgtcagg tgcgcagggt gtccgaagtc gcgggatggg gtcgcgccgt gcggcctgga
     3061 cgcgaattct ttggggcaat tgccccaggt ctggtgggcg atgtccccca ttcggatgtg
     3121 cgtcacccgg gctcttcgca ggagaagggg gcctctgaca ggttggcacg cagcgtgcgc
     3181 tgcacagtcc tgccatgtcg gcaaactgtg tcccggagcc ctgttcatgt tgaagcgcct
     3241 gctacccctt ctgaccctct tgcccgcgct ggcgccggct gcgcaggtcg tcttcaccaa
     3301 ctccacggac gtcaccacct acagcatggt gaccgtggac agcaggaagt tgaagaccgg
     3361 tcttgcggcc ggcctggtgg cctatccggg tgaggagtgc atcgcattca ccgacaccaa
     3421 cgtcgtaccg gtctgtggcg gcttcaccat cagcaatggc tggatgtacc ccaatgccgg
     3481 aggcttcccg ggttggcccg accccaacga tccgcgcaag gacctgcgga tcgacgggct
     3541 caagaccaag gacatcacca acttcctgag cccgggccag tgcacggcag ccggctgccc
     3601 tgcggcgccg ccgccggtgc cgctgacgct gaccttcacc cgtcccacgg tggaattcgg
     3661 aatgatgttc cgcgcctcct gggaaaccca gcaaacgccc ttcacgacag gcttccgtgt
     3721 catcgcaaac ggtgtggatc tgggcaccta cccggtaccc gtgcagggag tgcagacgct
     3781 gggcgtgttc gctcccgaag gcctgaaaac cgtgaccatc gttccctaca acatcgaccc
     3841 gaccgtcgtc ggtccgacgg tcattcaccg gatctacacc aagtaacagg aagcgctgcg
     3901 cccgctggcg cagcgttcag gccgcgtgca gcgcggccgc gttcttgcgc gtgagttcgc
     3961 cgcacagcgt cgcgaagcgc tggcgggtcg ggtacttgtc catcagcacg cgtgcgctgc
     4021 tgtggaactc cggcggaagc tggtgggcgt tggtcgagtt gtggaacaac ggcacgtcca
     4081 ggatctgcgc gatcggccgc ttggccttct gctcggcctg caggcacagg tcggtggccc
     4141 acaggtgcca gcccagtgca gggtcgatcg agacatcgct gtcgcgatgc agcgcgacgg
     4201 cgaattcgtc catcgacacg gcgccggccg agggctgatg ccggaacagc gtggtgcggt
     4261 cgatcaccag accggccttg cgcaccgtgc ccttgcgtgc cgggtcgggt tcgaggccgg
     4321 cgaagccgat cggcgcgccg atcagcccgg cctgttcgac tgcgccgagc tgctgtgcca
     4381 gcgcgtagcc gctgccggtc gggaagtaga cgtcctgatg ggccatcacg cgccacgcgt
     4441 gggtggcccg ctgcgatccg ctgacgtagg ctgcagccgc gctgctggcg ccctgcacgc
     4501 agatgacctc ggcatcgatt tcctgcaggc cgggtgagcg cgccacgttg agctcgtgct
     4561 gccacgggcg attcaccgga acgatgacgc tgaacggtac gcgcgggccg ggtcgcagca
     4621 gcgactgcag cggccacttc tcgcacacca ggatcatctg gtacagcccg aggttgtgca
     4681 ccgcggtctc catcggcagc cccagcgcca ccgcggcatc gacgatgcgc gcggcgaaat
     4741 gggtctgcgg cagcttgaac cggtactggt ccttcaggtg cggcatccag ccgctgtcga
     4801 ggaaggtctt gaacgccgac gacggcgtgt agaagcgcgt gtgcgtgcgg tcgagcaggc
     4861 cggccgcgtc gtagctgatg tctccggcga ccagccgttc gatgaccgac aggtggccca
     4921 tgttcggcag gcagcaggcg atgcgcgcgt cctcggtgcc gaggtcgtag agcgcgctca
     4981 gcacgcgccc cgggtcgcgc aggtgctcga gcaggtcgcc gatcacgatg acatcgaagc
     5041 cgccctccag caccgacaag tcggcgttgt ccaggtccag ctgcacggcg cggtccaggt
     5101 gctgcgaagc ggctgccacc gcgtctgccg acacgtcgac gccgcaccac tgcacgccgg
     5161 ggtgcagttc cttgaagcgg cgcccgagca ggccgttggc gcagccgagc tccagcacgc
     5221 gacgcgcctt cgcggggatg gcgtcgagca gcttgaggtt caggccgtcg tagtagttcg
     5281 tggcagcggg agtggcggtg ggtgcacgca tcgaggggct ccggttgggc ttcagcctgc
     5341 gacggcggcc tgcgccgcgc ggcgcagtgc ctcgctgcgc gtggtggcga gcaggtcgag
     5401 ggtggcgatg tcgaggccgc caggggcggt ggccgcgggg cgcgccgcag gcagcgtcgg
     5461 cgcgccgacg ggcgcggcgg cagccggcag gatcgacggc atcggtttca tcggtgcagg
     5521 cgagccggcg gcaggcgcag agtagacagg aaccaccggc ctgcgttctg gtgc
//
LOCUS       DAOORV010000213         5572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17597_length_5572_cov_6.123618, whole genome shotgun sequence.
ACCESSION   DAOORV010000213 DAOORV010000000
VERSION     DAOORV010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17597_length_5572_cov_6.123618"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKV56_17325"
     CDS             complement(<1..246)
                     /locus_tag="PKV56_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69618.1"
                     /translation="MKDTALHSSSTTPRNSARTRRSALKPDAYVPASTVRDLLAHSLD
                     ARQQRLGAADMISTDDAAQLVHTTRVTINAWIAKGRAI"
     gene            complement(296..670)
                     /locus_tag="PKV56_17330"
     CDS             complement(296..670)
                     /locus_tag="PKV56_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69619.1"
                     /translation="MIVWELVVGPGERDRSPDSCALSAYRDYVVARHLLESDFLPRRF
                     VSTRLSAAMVGAFQVVRRRSAFRAETLYSQWRQFVVLASALPARRRRKLRSSRSSAAP
                     LPTARRARAFFVAHKVDGRNAP"
     gene            1009..1218
                     /locus_tag="PKV56_17335"
     CDS             1009..1218
                     /locus_tag="PKV56_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69620.1"
                     /translation="MKPALPIPEDALFFERDGLPVFSTPSMGYRSAVTGVVLRSPYEL
                     MPVSREVFEKLLADWPASDSSRDEK"
     gene            complement(1285..1635)
                     /gene="tnpC"
                     /locus_tag="PKV56_17340"
     CDS             complement(1285..1635)
                     /gene="tnpC"
                     /locus_tag="PKV56_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase post-transcriptional
                     regulator TnpC"
                     /protein_id="HOX69621.1"
                     /translation="MSDSEDTIVTPYGRLDRAALLALENTYDTRVLLSAVDQLDKVLG
                     VARGQNGLRDMLLRLHRMAQIVINGAGLAGDTGGETLPELAFDTTAELRELIATLQRW
                     VKQIEPLEGLQPRG"
     gene            complement(1632..4661)
                     /locus_tag="PKV56_17345"
     CDS             complement(1632..4661)
                     /locus_tag="PKV56_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOX69622.1"
                     /translation="MASIERTAYPRFPRALTLKDLQTSFTPRPDEADWALRYARRPQG
                     RLALLVLLKCFQYLRHFPTVEAIPPEVVEQVSATLGVTPEQTIQYAGSRTALYRHHTA
                     IRELLGVQPYTDAQTRKLAIRIAQEAAAVVETRVDIINMVIEELVRQGYELPVFRTLD
                     DIAEQAHAFAQAELHVRIAQRLTAQQRKWLDQLIEAELPARRTLYNQLKRTAKKASRK
                     HLGLLLDQLRWLESLPDSSALLADVPATKLKHMAEMAAVLDAGDMKDLRPAKRHALIL
                     ALIRQMSIRARDDLAEMFIRRLGAIHRNARDELQVIQARQRETSEELVATLEQVLVIL
                     AENLDDAATGQRVRELLAPHGSLDKLRADCEAIRVWSGGNHLPLTWKPFSSWRAAMFR
                     MAEVLDFEAATQDRSLLKALQVVIDNENRKAEWITDEVDMSFASERWRKLVRRSHALG
                     NPTNRRYLEVCVFSHLSNDLRSGDVCIAGSESFADHRKQLLSWEECQARLSDYCDRMG
                     LPADAAGFVDGLKKLLTDTAGEVDREFPQHAGDVVIGSSGEPTLRRATARETPASAIA
                     LQAAIENRTAARNLLDILANIEHWTGFTRNFGPQSGDDPKLRNARERYLLTVFAMGCN
                     LGPTQAARHLSNGVTPHQLSYANQRHMGLEQLDNACRDLTELYLRLELPKLWGDGKKV
                     AADGTQYDFYEQNLLVGMHFRYRKVGAVAYRHVADNYIAVFRHFMPPGVLEAVYVIEG
                     LTKAGLSVQADTVYSDTHGQSETVFAFTHLLGIQLMPRIRNWKDLHFYRPEKGTKYRH
                     IDRLFTEVVDWKLIRDHWQDLMQVAISIQAGRIASPMLLRKLSHEGRHNRLFAAAREL
                     GRVLRTVYRLRWISSKEMRQEVSATTNKIESYHAFTKWLDFGGDVITENDPNEQQKRV
                     RYIDLVAAAVILQNTVDMMRVLQELYAAGEPISVADVEYLSPYMTYGVKRFGNYHLDL
                     KRPPEPWVKESQFRDAAKRARAAAAEAQGTGTSTGAT"
     gene            4712..4891
                     /locus_tag="PKV56_17350"
                     /pseudo
     CDS             4712..4891
                     /locus_tag="PKV56_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
     gene            5171..5437
                     /locus_tag="PKV56_17355"
     CDS             5171..5437
                     /locus_tag="PKV56_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69623.1"
                     /translation="MRIVLVVGAHGVTAFQADADESQRRRFDDFSRAKPNVEGRRFKR
                     STAQEQARRERPRRERQSVVWLRTDDASLLDLSAPVRYELRPVR"
BASE COUNT          925 a         1772 c         1860 g         1015 t
ORIGIN      
        1 aatcgcacgc cccttggcaa tccaggcatt gatggtgacg cgggtcgtgt gcaccaattg
       61 ggcggcgtcg tcggtcgaga tcatgtcggc tgcgcccagg cgctgctgtc gagcgtcgag
      121 gctgtgggcc agcagatcgc ggacggtgga agctggaaca taggcatccg gcttgagggc
      181 gcttcggcga gtacgtgcag agttgcgagg agtggttgaa gacgagtgga gagcggtgtc
      241 tttcatttcc agcctttggt ctggaggccg tgacgacggc ccgaggaaga gcgccttacg
      301 gcgcgttgcg gccgtcgacc ttgtgggcga cgaagaaggc cctagcgcga cgcgcggtcg
      361 ggagaggagc ggcagacgat cgactggacc tcagcttcct gcgccggcga gcgggcaagg
      421 cggaggcaag cacgacgaac tgacgccact gggaatagag ggtctcggcg cgaaatgctg
      481 accggcggcg aacaacctgg aaggcaccca ccatggcagc cgacaggcgc gtcgagacga
      541 accgacgcgg gaggaagtcg gactcgagca ggtgacgtgc gacgacgtag tcccggtagg
      601 ccgacagagc gcaggagtcg ggcgagcggt cgcgctcacc cggtcccacc acaagttccc
      661 aaacgatcat ttcaggtcct ccagtgcccc gacatgcgcg aggccgacga acaagaagga
      721 tgtcaagttc gttaagttgg attatgccgc aagttctggt gacaacaacc ggtggtcgca
      781 gacagcacca acccgggcac aggctgtgaa gcgaatcgcg tgatggagaa cgagaccggt
      841 tggattcgac gcttgggcag tcgaccacga gggtgcagag tcgccgaacc acgtgagggt
      901 caggcaagat ggcgcgcgag ccgcaggagg gggtgccacc ttgctgccgt ggccaacacc
      961 ttgcaacgcc tggacgcagc gaacgctcga ggacgtggag cacgatcgat gaagccagcc
     1021 ctgccgattc ctgaagatgc actgttcttc gagcgtgacg gcttaccggt cttcagcaca
     1081 ccgtcgatgg gctaccgttc ggcggtcacc ggcgttgtcc tgcgctcgcc ctacgagctg
     1141 atgcccgtca gccgcgaggt gttcgagaag ctcctggcgg attggcccgc gtccgacagc
     1201 tcgcgcgacg agaagtagac gcgcttcgag ttgggggtaa gccgggataa agccagaaat
     1261 cacacacagg cctgacggcg cctgtcagcc ccgtggctgc aggccctcca gcggctcgat
     1321 ctgcttgacc caccgctgca gcgtggcgat cagctcccga agctccgcgg tggtgtcgaa
     1381 cgccagctct ggcagcgtct ccccgcccgt gtccccggcg aggccggcgc cgttgatcac
     1441 gatctgcgcc atgcggtgca atctcagcaa catgtcgcgc agcccattct ggccacgcgc
     1501 gacaccgagg accttgtcca gctgatcgac cgcgctcagc agcacgcgcg tgtcataggt
     1561 gttctccagc gccagcaacg cggcccgatc caagcgcccg taaggcgtga cgatggtgtc
     1621 ttctgaatcg ctcatgtcgc tcccgttgat gttcccgtgc cctgcgcctc ggccgccgca
     1681 gcccgcgccc gcttggcggc gtcgcggaac tgcgattcct tgacccacgg ttccggtggg
     1741 cgcttcaggt cgaggtggta gttgccgaag cgcttcaccc cataggtcat gtacgggctt
     1801 aggtactcca cgtcggccac cgaaatcggt tcgccggccg cgtaaagctc ttgcaacacc
     1861 cgcatcatgt ccaccgtgtt ctgcaggatc acggccgcgg ccaccaggtc gatgtagcgc
     1921 acgcgcttct gctgctcgtt cgggtcgttc tcggtgatga catctccacc gaaatccagc
     1981 cacttggtga aggcgtggta cgactcgatc ttgttggtcg tggcgctgac ctcctggcgc
     2041 atctccttgc tggagatcca gcgcagccgg tagaccgtgc gcagcacccg tcccagttcg
     2101 cgcgccgcgg cgaacaagcg gttgtgccgc ccttcatggc tgagcttgcg caacagcatc
     2161 ggcgaggcga tgcgcccggc ctggatcgag atggccacct gcatcagatc ctgccagtgg
     2221 tcgcggatca gcttccagtc caccacctcg gtaaagagcc ggtcgatgtg gcggtacttg
     2281 gtgcccttct ccggccggta gaagtgcaag tccttccagt tgcggatgcg cggcatcagc
     2341 tggatgccca gcaggtgcgt gaacgcgaac accgtctctg attgcccatg ggtgtcggaa
     2401 tagaccgtgt ccgcctgcac cgaaaggccg gccttggtca ggccttcgat cacatagacc
     2461 gcttccagca cacccggcgg catgaaatgc cggaacaccg cgatgtagtt gtcggccacg
     2521 tgccggtagg cgaccgcgcc caccttccgg taccggaaat gcatgcccac cagcaggttc
     2581 tgctcgtaga agtcgtactg cgtgccgtcg gcagccacct tcttgccgtc gccccacagc
     2641 ttgggcagtt ccagacgcag gtacagctcg gtcaggtcgc ggcaggcgtt gtccagttgc
     2701 tccaacccca tgtggcgctg gttggcatag gacagctggt gcggcgtcac cccgttggac
     2761 aggtgccgtg ccgcctgtgt cggcccgagg ttgcagccca tggcgaagac ggtgagcagg
     2821 tagcgctcgc gcgcgttgcg cagcttcggg tcatcgcccg actgcggccc gaagttgcgt
     2881 gtgaaccctg tccaatgctc gatgttggcc aggatgtcga gcaggttgcg cgctgcggtg
     2941 cggttttcga tcgctgcctg cagcgcaatc gcgctggcgg gagtctcgcg tgcggtcgcg
     3001 cggcgcagcg tcggctcccc gctgctgccg atcaccacgt cgccggcgtg ctgcgggaac
     3061 tcacggtcga cctcgcctgc ggtgtcggtc agaagtttct tgaggccgtc gacgaagcca
     3121 gccgcatcgg ccggcaggcc catgcggtcg cagtagtccg acaggcgggc ctggcattcc
     3181 tcccacgaca gcagctgctt gcggtggtcg gcgaaggact ccgagccggc gatgcacacg
     3241 tcgccgctgc gcaggtcgtt gctcagatgg ctgaagacgc agacctccag gtagcgccgg
     3301 ttggtggggt tgcccaaggc atgagaacga cgtaccagct tgcgccagcg ctccgaggcg
     3361 aaggacatgt ccacctcgtc ggtgatccac tcggccttcc gattctcgtt gtcaatcacc
     3421 acttgcagcg ccttgagcag gctgcgatcc tgcgtcgcgg cctcgaagtc cagcacctcg
     3481 gccatgcgga acatcgctgc ccgccagctg ctgaacggct tccaagtcag cggcaggtgg
     3541 ttgcccccgc tccacacccg gatcgcctcg caatcggcgc gcagcttgtc caggctgccg
     3601 tgcggtgcca gcagctcgcg cacgcgctgg ccagtggccg cgtcgtcgag gttctcggcc
     3661 agaattacca gcacctgttc gagcgtcgcc accagttctt cgctggtttc tcgctggcgg
     3721 gcctgaatga cctgcagctc atcgcgtgcg ttcctgtgga tggcgccgag gcgccgaatg
     3781 aacatttcgg ccaggtcgtc gcgggcgcgg atgctcatct ggcggatcag ggccagaatc
     3841 agcgcgtgcc gtttggccgg tcgcagatcc ttcatgtcgc cagcgtccag caccgcggcc
     3901 atctccgcca tgtgcttgag cttggtggcg ggcacgtcgg ccagcagcgc gctgctgtcg
     3961 ggaagcgact ccagccaccg cagttggtcg agcaacagcc caaggtgctt gcgcgacgcc
     4021 ttcttggccg tgcgcttgag ctgattgtag agcgtgcgcc gcgccggcag ttcggcttcg
     4081 atgagctggt cgagccactt ccgctgttgg gccgtcaggc gttgggcaat gcgcacatgc
     4141 agctcagcct gggcaaacgc atgggcttgt tcggcgatgt catccaaggt acggaacacc
     4201 ggcagctcgt agccctgccg taccagctcc tcgatgacca tgttgatgat gtcgacgcgg
     4261 gtctcgacga cggccgcagc ctcctgcgca atccggattg ccagcttgcg cgtttgggca
     4321 tcggtgtagg gctgcacgcc cagcagctca cggatcgcgg tgtggtggcg gtacagggct
     4381 gtccgcgagc ctgcgtactg gatcgtctgt tcaggcgtca cgccgagcgt ggccgacacc
     4441 tgttcgacca cctcgggcgg gatggcttcc accgtcggga agtggcgcag gtactggaag
     4501 cacttgagca gcaccaggag cgccagccga ccttgcggcc gacgtgcata gcgcagcgcc
     4561 cagtcggctt cgtcggggcg tggcgtgaag gaggtctgca agtccttgag cgtcagcgcc
     4621 ctcgggaatc gagggtatgc ggtgcgttca atgcttgcca tgcctcgttg ctcccaccac
     4681 gtccagtgtt gccgagcgcc cagcctaccc gatcgtcgcg caggtgatgg ggcacctgcc
     4741 gctgtccaca ttccggcgct gtgtggcgcg ctacgacggc gagcacaagg gcacgagctt
     4801 ctcgtgcctg gatcagttct acacgatggc gttcgcgcaa ttgacgtttc gagagtcgct
     4861 gcgcgtcatc gaggggtgtt tggccaccca ggtgcaggcc gtcgtcgatg aaccccggcc
     4921 gtgttgtgct ctgggcttcg tggaagagcg caaaacgatc ctcccagtga ttaccctggg
     4981 gctaccggag ctggaggatt ctgcacgatc ttggcaacgg gttccgtctg ggccttcaag
     5041 gcgcgaaggg ccttggcgcc gagaccctgc tgctggactt cgagttccag gggtgtgcgc
     5101 cgctgcaggg ggcgctcgat cctgcggatc gctaccgtga gacgcgtgct gggcgcattc
     5161 ggaaattccc ttgcgtatcg tgctcgttgt cggcgcccac ggtgtgacgg catttcaagc
     5221 agatgccgat gagtcgcagc gccggcggtt cgacgatttt tcgcgggcga agccgaacgt
     5281 cgagggacgt cgcttcaagc gatccactgc acaggagcag gcgcggcgcg agcgccctcg
     5341 ccgcgagcgg cagtcggtgg tctggctgcg caccgacgat gcgagcctgc tcgacttgtc
     5401 agcaccggtg cggtacgagc tgcggccggt tcgctgatcc aaccacgatc agaatgcaca
     5461 actggatgcg agcggcgtca actgacaacg cccaccaagc gctgcgacgc ctgtcaacct
     5521 aaatccggca gttaccccgt cgcctgaagc gctggcgggg gcttcggcgg gc
//
LOCUS       DAOORV010000214         5497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_17877_length_5497_cov_9.579199, whole genome shotgun sequence.
ACCESSION   DAOORV010000214 DAOORV010000000
VERSION     DAOORV010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17877_length_5497_cov_9.579199"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(683..1195)
                     /locus_tag="PKV56_17360"
     CDS             complement(683..1195)
                     /locus_tag="PKV56_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69624.1"
                     /translation="MRIKLIEDLPMASSAELYQLSWVIEQLLADPRRIVQARAELHTG
                     QQVQYLDWDDGKMRAARVVAMKDRRVTLLDEASRSHFTLPYGAIVPAPSGGPDSGAEA
                     PLPAKPTPPPEIASRADFRVGNRVSFTDQNLQHRVGLIVRINQRTATLDCDGQTWRVG
                     FGLLRPLVDI"
     gene            complement(1230..2852)
                     /locus_tag="PKV56_17365"
     CDS             complement(1230..2852)
                     /locus_tag="PKV56_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOX69625.1"
                     /translation="MTSVDLHASNAAPSGGVGGELQRTQESLKIALLTIDKLKVELAY
                     LRRMKYGRSSEQMQNAQLELVGGMVVTPAAEPASNVTSMEAGRKKRTPKPRPGLREFP
                     EHLPRRTVVHAPPSGHDGGCDCQACGRALREIGQDVSEVLDYEPGSFHVVRHVRPRLA
                     CTGCKTITQAAAPSRPVQRGMAGPGLLAHVLVGKYADSLPLYRQCQIYAREGVLLERS
                     TLTDWVGQAARLLSPLAQAIGRHVLHADKIHGDDTPIRVLGGKGSRAKTGRLWVYVRD
                     DRPHQGSNAAATASPAVWFQYSADRKGEHPARHLLGWRGILQADAFAGYNHLYSDGRI
                     IEAACWSHARRKLWDIHERQHKMTGTLAHQGLERIAKIFKIEAEIRGQSSLRRRRVRQ
                     LRTRPVLDELKAWMTQTLTQVSAKSPLALAIGYAASNWTALVRFASDGRIEAENNAAE
                     RALRAVAIGRKNFLHLGSDAGGNSAAVIYSLIGTAKLNGINPQHYLRYVLERIADHKV
                     NCIDELLPWAVAERIALDTQQVQPEQELPLAA"
     gene            complement(2936..3283)
                     /gene="tnpB"
                     /locus_tag="PKV56_17370"
     CDS             complement(2936..3283)
                     /gene="tnpB"
                     /locus_tag="PKV56_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOX69626.1"
                     /translation="MIAMRSGTRVWLAAGVTDMRCGIDSLAVKVQTVLAEDPYAGHVF
                     AFRGRRGDLVKLLWSDGDGMCLLTKRLERGRFVWPQAHSGSVALSAAQLSMLLEGIDW
                     RRPERTWQPRAAA"
     gene            complement(3294..3761)
                     /locus_tag="PKV56_17375"
     CDS             complement(3294..3761)
                     /locus_tag="PKV56_17375"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX69627.1"
                     /translation="MKTVAGIAGAPRRQHEKSFKAELVERCLLPGASVAAVALAGGVN
                     ANLLFKWRRDHLRSMRLSSAASSTSSVLVPVRVAAFGHADADHAQSPAPAPAPAPAPA
                     PAPAPAALTVAPQRCARNTGTIELDIAGAVLRLCGPVDEVSLCSVLRALRQST"
     gene            complement(3811..4209)
                     /locus_tag="PKV56_17380"
     CDS             complement(3811..4209)
                     /locus_tag="PKV56_17380"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOX69628.1"
                     /translation="MRHVSDWTLDNGSLLLNFSDEVRGVFERQVQVGDLPESGGVLLG
                     TVHENGLLVTLATTPSRLDRQLRYLFERLPFGHRAVAKRLWRSTGGTTRYIGEWHTHP
                     QDIPVPSDIDRNSPTTPVLASPLAAGQHLT"
     gene            complement(4197..>5497)
                     /locus_tag="PKV56_17385"
     CDS             complement(4197..>5497)
                     /locus_tag="PKV56_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOX69629.1"
                     /translation="RAEQVLADILAGKMVEDLAEEFHITWGHRWCMLDFDVRRPGGLN
                     AYTYGGKISVATDDKQRTHAKLEAIGLGLPKTDLKAFRVHTKARPMPLQESWPPKTVQ
                     EFLKWQNTLDPKCSKKIEQRLLELFRAKAKSAMVLIDSPQVQYGVEVDLQRGTAGISK
                     RPSLRETLYRLPIHPISAYRIDDPYLAERNLPGSKTLAGLKIGLVGCGTIGGYLAEML
                     AKAGGGTVGGKLTLVDLGSMEPGNLGRHRLGFNALIKKKSEAMCEELRRVAPGIDAAA
                     VVGDVKDANLEPLDLLIDATGEQGLTDWLTWQYADKVPFLAAWVEGAGAAVRALLKAK
                     PEHACARCVSQPPLKNQYRVFDAPPEVIMKGNGCEGLYVPFPATASVQAAALAMEMVQ
                     AWLDGAESPSFRTRVLDLKREVHFKDCSPHRYEGCPACAT"
BASE COUNT          853 a         1820 c         1828 g          996 t
ORIGIN      
        1 cttctgcccg acgatcttgc ccttgttcca cggttcgcga cgagcgttgt tgcttgtggt
       61 gcccatgatg gtctcccatc gagttgaggg ccacacaaga tgcgctgact ccccagcgga
      121 gcccttgcct tgggtcaaca gtaaacccat cgcggaccgg accgatggac cgcttactgg
      181 ctccggcagc tactcacgct gccggccatc agttgacgct ggtgacggcc ggctatatgg
      241 aagtccaatg gggccgaaca agtcgagccg ctggcatggc caaggaagtt ctgctgtgcc
      301 cgcaggcagc gtgcacacgg tggcctccag cggcaggttt ctgaatcggc acacgcgccg
      361 gggcggtgac ccgcgtcgtc gcagacttgg ccatgcattg agcacccatt ccgagccggc
      421 tgccagatcg gccggccacc gttcgtggca cccatgatgg cggaagagtg catatgttcg
      481 acaccgaaca gacgccgcaa ttgccgatgg gccatcctcg gcttcctttg tcgaagtgac
      541 ccttctggac gcccgtccag ctcaactcta ggaactcaac atgggcccga cacgcgtgct
      601 tgttgtcgat gacaacgaaa ccttcagcga gatggcgaag ttgtaatagt ccgaccattg
      661 ccgtcattga cggcggcccc gttcagatat caacgagagg ccggagcagg ccgaagccaa
      721 cgcgccaggt ctgaccatcg caatccagcg tcgcagtgcg ctggttgatg cgcacgatca
      781 agccgacgcg gtgttgcagg ttctggtcgg tgaagctcac gcgattgccc actcggaagt
      841 cggcgcggct ggcgatctcg ggcggtggtg tgggcttggc cggaagcggg gcttcggcgc
      901 cgctgtcggg tccgccggac ggcgcaggca cgatggcccc gtagggcaac gtgaagtggc
      961 tgcggctggc ctcgtcgagc aaggtgacgc ggcggtcctt catggccacc acgcgcgcgg
     1021 ccctcatctt gccgtcgtcc cagtccaggt attgcacctg ctggccggtg tgcaactccg
     1081 ctctggcctg cacgatgcgc cgcgggtcgg ccagcagttg ctcaatcacc cagctgagct
     1141 ggtacagctc ggcgctgctg gccatcggca ggtcttcgat cagcttgatg cgcatgcgca
     1201 cattctgcca accgtgacgg cgccggcgct caggccgcca gtggcaattc ctgctctggc
     1261 tgcacttgct gtgtatcgag ggcgatccgc tcagccaccg cccagggcaa caactcgtcg
     1321 atgcagttga ccttgtggtc ggcaatgcgc tcgagcacgt agcgcaggta gtgctgcggg
     1381 ttgataccgt tgagcttggc cgtgccgatc aggctgtaga tcacggcggc gctgttgcca
     1441 ccggcgtccg agcccaggtg caggaagttc ttgcggccga tggccaccgc cctgagcgct
     1501 cgctcggcgg cattgttctc ggcctcgatg cgcccgtcgc tggcgaagcg caccagggcc
     1561 gtccagttgc tcgccgcata gccgatggcc aatgccagcg gcgacttcgc cgagacctgc
     1621 gtcagggttt gggtcatcca ggccttcagc tcgtccagga ctggccgcgt tcgcagctgc
     1681 cgcacccggc ggcgtcgcag ggatgactgg ccccggatct cggcctcgat cttgaagatc
     1741 ttggcgatgc gctccagccc ctgatgggcc agcgtgccgg tcatcttgtg ctggcgctcg
     1801 tggatgtccc acagcttgcg tcgggcatgg ctccagcatg cggcctcgat gatccggccg
     1861 tcgctgtaga gatggttgta gccggcaaac gcatcagcct gcaaaattcc ccgccagccc
     1921 aagaggtgcc gcgcggggtg ctcacccttg cggtcggccg agtattggaa ccacaccgca
     1981 ggcgatgcgg ttgccgctgc gttcgaaccc tgatgcggcc ggtcatcgcg cacatagacc
     2041 cacagccggc ctgtcttggc ccggctgccc ttgccgccca gcacccggat cggcgtgtcg
     2101 tcgccgtgga tcttgtccgc gtgcagcaca tgccggccga tggcctgggc cagcgggctc
     2161 agcagccgag cggcctggcc gacccagtcg gtcagggtcg agcgctccag cagcacaccc
     2221 tctcgggcat agatctggca ctgccggtac agcggcaggc tgtcggcgta cttgcccacc
     2281 agcacatggg ccagcaagcc agggccggcc atgccgcgtt gcaccgggcg gctgggtgct
     2341 gcggcctggg tgatcgtctt gcagcccgtg caggccagtc gggggcgcac atggcgcacg
     2401 acgtggaagc tgccgggttc gtagtccagg acctctgaga cgtcctggcc gatctcgcgc
     2461 agcgcgcgtc cgcaggcctg gcagtcacag ccaccgtcat ggccggacgg cggggcgtgc
     2521 acgacggtgc ggcgcggcag gtgttcggga aattcgcgca ggcccggacg cggcttgggc
     2581 gtgcgcttct tgcggccagc ctccatcgag gtgacgttgg atgccggctc ggccgcaggc
     2641 gtcaccacca tgccgccgac cagttcgagc tgggcgttct gcatctgctc gctggagcgg
     2701 ccgtacttca tgcgccgcag gtaggccagt tcgaccttga gtttgtcgat ggtcagcagc
     2761 gcgatcttca aggactcctg cgtgcgctgc agttcaccac caaccccacc gctgggagcg
     2821 gcgttgctgg cgtgcaggtc aacggatgtc atgaggctga ctgtggcagc gcagttcttc
     2881 acccgcaatg ggatatgtcc cgtcaaccag tcccgtcaac cagttccggt ctgcgctacg
     2941 ccgctgcgcg cggctgccag gttcgttcgg gccgtcgcca gtcgatgcct tcgagcagca
     3001 tcgacaactg cgcggcgctc aatgcgacgc tgccgctgtg ggcctgcggc cagacgaagc
     3061 gtccgcgttc taagcgcttg gtcagcaggc acatgccgtc gccgtcgctc cacagcagtt
     3121 tgaccaggtc accgcggcgg ccgcgaaagg cgaagacatg gccggcgtac gggtcctctg
     3181 ccaacacggt ttgcaccttg acggccaggc tgtcgatacc gcagcgcatg tcggtgacac
     3241 cggcggccag ccagacccgg gtgcccgatc tcatggcaat catggccggc tgatcaggtg
     3301 ctctggcgca gcgcgcgcaa cacgctgcac aggctgactt cgtcgaccgg gccgcacagg
     3361 cgcaacacgg cgcctgcgat gtcgagttcg atggtgccgg tgttgcgtgc gcagcgttgg
     3421 ggcgcaaccg tcaatgcagc cggtgccggt gccggtgccg gtgccggtgc cggtgccggt
     3481 gccggtgccg gtgattgcgc atggtcagca tcggcatgcc cgaacgcagc gactcgcact
     3541 ggcaccagaa cggacgatgt cgatgacgcc gcgctgctca accgcatcga gcgcaggtga
     3601 tcgcggcgcc acttgaacag caggttggcg ttgactccgc cggccaatgc aacggccgcg
     3661 accgacgcgc cgggcaacag gcatcgctcg accagctccg ccttgaacga cttctcgtgc
     3721 tgacgccgag gcgctcccgc aatccctgcc actgtcttca tggtgtgcac catctcctgc
     3781 tggtgcacac catcaagtgg gcggcactct tcaggtcaga tgctgacccg ctgctagcgg
     3841 cgacgcaagg acgggggtgg tcggactatt acgatcgatg tcggaaggga cggggatgtc
     3901 ctgtgggtgg gtatgccact cgccgatgta gcgggtagtg ccaccggtgg aacgccagag
     3961 gcgcttggcc accgcgcggt gaccgaacgg caaccgttcg aagaggtagc ggagctgtcg
     4021 gtccaggcgc gacggcgtgg tggccagggt gaccagcagc ccattctcat ggacggtgcc
     4081 cagcagcacg ccgccgctct ccggcaggtc accgacctgg acttgcctct cgaacacgcc
     4141 acggacctcg tcggagaagt tcagcagcag gctgccgtta tccagcgtcc agtcactcac
     4201 gtggcgcatg cggggcatcc ctcgtaccgg tggggtgagc agtccttgaa atggacctcg
     4261 cgtttcagat cgagcacccg cgtcctgaag ctgggcgact cggcgccgtc cagccaggcc
     4321 tgcaccatct ccatggccag ggcggccgcc tggacggatg cggtggcagg gaaggggacg
     4381 tacaggcctt cgcagccgtt ccccttcatg attacctccg gcggcgcatc gaacacgcgg
     4441 tactggttct tcagcggcgg ctgcgagacg catcgcgcgc aggcgtgttc gggcttggcc
     4501 ttcagcaagg cgcggacggc tgcgccggct ccctcgaccc aggctgccag gaacggcacc
     4561 ttgtcggcgt actgccaggt gagccagtcg gtcaggccct gttcgccggt ggcgtcgatc
     4621 agcaggtcca ggggctccag attcgcatcc ttgacgtccc ccaccacagc tgccgcgtcg
     4681 atgccgggcg cgacgcgcct gagctcctcg cacatcgcct cggatttctt cttgatcagg
     4741 gcgttgaaac cgagccgatg gcggccgagg ttgccgggct ccatcgaccc caggtccacc
     4801 agtgtcagct tcccgcccac cgtgcccccg ccggccttgg ccagcatctc ggcgaggtag
     4861 cccccaatgg tcccgcagcc gaccagcccg atcttgagcc cagccagggt cttcgagccc
     4921 ggcaggttgc gctcggcgag ataggggtcg tcgatgcggt acgcggaaat cgggtggatc
     4981 ggcagccggt agagggtttc gcgcagagac ggccttttgc taatgccggc ggtgccgcgc
     5041 tgcaggtcca cctcgacgcc gtactggacc tggggcgagt cgatcaggac cattgcgctc
     5101 tttgccttcg cccggaacag ttccaggagg cgctgctcga ttttcttgct gcacttcggg
     5161 tccagcgtgt tctgccactt caggaactcc tgaactgtct tgggcggcca gctttcctgc
     5221 agcggcatgg gccgtgcctt ggtgtgcacg cggaacgcct tcaagtcggt cttgggcagc
     5281 cccagtccga tggcctccaa tttggcgtgg gtgcgctgct tgtcgtccgt ggcgacgctg
     5341 atcttcccgc cgtaggtgta ggcgttaagc ccgccgggtc ggcgcacgtc gaagtccagc
     5401 atgcaccagc ggtggcccca ggtgatgtgg aactcctccg ccaggtcttc gaccatcttg
     5461 ccggccagga tgtcggccag cacctgctcg gcacggt
//
LOCUS       DAOORV010000215         5456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18028_length_5456_cov_10.413812, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000215 DAOORV010000000
VERSION     DAOORV010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18028_length_5456_cov_10.413812"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="dcd"
                     /locus_tag="PKV56_17390"
     CDS             <1..551
                     /gene="dcd"
                     /locus_tag="PKV56_17390"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOX69630.1"
                     /translation="WIRRMAEQHGMIEPFEPGQVRLNAAGEKIVSYGTSSYGYDIRCA
                     PEFKVFTNIYSTVVDPKNFDEKSFVDINADVCVIPPNSFALARTLEYFRIPRNVLTIC
                     LGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFFESDEVC
                     ETSYKDRGGKYQGQKGVTLPKT"
     gene            589..1479
                     /locus_tag="PKV56_17395"
     CDS             589..1479
                     /locus_tag="PKV56_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOX69631.1"
                     /translation="MKWEGNRQSDNVEDARSDGGGGGGGFRIGGGGRGIGIGTIVVAL
                     LGGWIFGINPLTILGALDGGSGGLAPQVRQAPAARPPADDPMAAFVSTVLADTEDVWK
                     AQFARAGSAYQEPKLRLFRGSEPTACGQGDSAMGPFYCPGDQKVYIDLAFFDTMSQRL
                     GAPGDFAQAYVIAHEVGHHVQNLLGITGKVDAMRGRVSEAEQNAMSVRVELQADCFAG
                     VWAHDSQQSKGWLESGDVEEALNAATQIGDDNLQRQSRGTVVPESFTHGTSAQRVKWF
                     QRGLQGGSLKQCNTFDNKAL"
     gene            1526..2017
                     /locus_tag="PKV56_17400"
     CDS             1526..2017
                     /locus_tag="PKV56_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69632.1"
                     /translation="MSNIQYAFIERGRVPSRSELQASIDALGFDLRLPPEYTPFEDSG
                     FLPFTLNGETGPGFEIQYDAASELIGEDQALREMAAGRDHCVSMSWHGSMKDLACVLI
                     VSCALMKDFDSVVSYEGEPPEPLESLLSVVPEVLADARAQEARDANRSARPEKKKPWW
                     KLW"
     gene            2097..2426
                     /locus_tag="PKV56_17405"
     CDS             2097..2426
                     /locus_tag="PKV56_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOX69633.1"
                     /translation="MYSSTFIFAKGQWDDEFHRLDEEIAQAAKATPGYLGEESWENPA
                     QGLISNVYYWESLEALQALMRHPAHLQAKARQADWLDGYQVVISQVLRTYGDSRLAGK
                     LPTAGAA"
     gene            complement(2471..2965)
                     /locus_tag="PKV56_17410"
     CDS             complement(2471..2965)
                     /locus_tag="PKV56_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOX69634.1"
                     /translation="MTTNDMDSVELFLAYSIKLEEEAALRFDQLADAMISAGNREVGK
                     LFRRLADYSRLHMRTARDRSGFHELPAFRPGDFEWPDIESPETAAIWAADPFIGREQA
                     LQVALDAESASLDYYREILESTQDPEVRHFAREFVDEETEHVAELQRWLALHKAGKPL
                     PIDR"
     gene            3318..4229
                     /locus_tag="PKV56_17415"
     CDS             3318..4229
                     /locus_tag="PKV56_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX69635.1"
                     /translation="MANVTFSSEVMPRDITVYAVAGDRGNLLALAKEHHIPIPFDCQD
                     GECGSCLVEVKHLTKGVRYGIALTEKEKELLKQLGKITKEQLMDAEVNDMPPPFRLAC
                     QCFVRDEDTIVTFEGDKTLPVKGPTLSIAAAVYKGGVQVHTMDDFLAYAVKVEEDAAV
                     HFEQLAKAMADCGNKDVAALFERLGSYSRLHLEEAKAKCKRHDVTLTLPPSSAWPDSE
                     TPEKTTLWAGDPSLSRLGALKVALQGERRGFEFYYAIAGTTTDADIRSVAKEFVREET
                     AHVEELKRWIEQEESALRMKADASATA"
     gene            complement(4291..4713)
                     /locus_tag="PKV56_17420"
     CDS             complement(4291..4713)
                     /locus_tag="PKV56_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69636.1"
                     /translation="MQIGVDTQRAVESKRAFIIDSDDISAMALQFMLADELEVNVVTC
                     SAEAIGRAQGLALDVILLGASVIEAEGTAVVTRLQAALPGVPVIACGPAGDPGIAQVL
                     ALGARSAIVRPYKVEAVRQRVNAQIGRRAVLTIPVVLG"
     gene            4937..>5456
                     /locus_tag="PKV56_17425"
     CDS             4937..>5456
                     /locus_tag="PKV56_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX69637.1"
                     /translation="MSLNTAYVDASALGKSRHAVSQILETAASGGCGTTGGEGKASCG
                     TSAGPDDMPQEIWDKVKNHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCSNES
                     RPGVVSQKLTPEQAVKKVVAVASEIPQMTVLGIAGPGDALANPKKTFDTFRMLQEQAP
                     DIKLCLSTNGLAL"
BASE COUNT         1012 a         1731 c         1784 g          929 t
ORIGIN      
        1 agtggatccg ccgcatggcc gagcagcacg gcatgatcga gccgttcgag cccgggcagg
       61 tgcgcctgaa cgccgcaggc gagaagatcg tcagctacgg cacgtcgagc tacggctacg
      121 acatccgctg cgcgcctgaa ttcaaggtct tcaccaacat ctacagcacc gtcgtcgatc
      181 cgaagaactt cgacgagaag agcttcgtag acatcaacgc cgacgtgtgc gtcattccgc
      241 cgaacagctt tgcgctggcg cgcacgctcg aatacttccg catcccgcgc aacgtgctca
      301 cgatctgcct gggcaagagc acctatgcgc gctgcgggat catcgtcaac gtgacgccgt
      361 tcgagccgga atgggaaggc tacgtgacgc tcgagttcag caacaccacg ccgctgccgg
      421 ccaagatcta cgccggcgag ggatgcgcgc aggtgctgtt cttcgagagc gacgaggtct
      481 gcgagaccag ctacaaggac cgtggcggca agtaccaggg tcaaaaaggc gtgacactcc
      541 cgaagacttg aaccgcgggt tctcgctgca gattccggag gtgtcgcgat gaaatgggaa
      601 ggcaatcgac agagtgacaa cgtcgaggac gcgcgctccg acggcggcgg tggcggcggc
      661 gggtttcgca tcggtggtgg cggacgtggc atcggcatcg gcaccatcgt cgtcgccttg
      721 ctcggcggct ggatcttcgg catcaatccg ctgacgatcc tcggcgcgct cgacggggga
      781 tcgggcggtc tggcgccgca ggtccgtcag gcaccggccg cccggccgcc tgccgacgat
      841 ccgatggccg cgttcgtatc gaccgtgctc gcggataccg aggacgtctg gaaggcacag
      901 ttcgcgcgcg ccggatcggc ctaccaggag cccaagctgc ggctgttccg cggcagcgaa
      961 ccgacggcgt gcgggcaggg cgactccgcc atgggtcctt tctattgccc cggcgatcag
     1021 aaggtctaca tcgacctggc cttcttcgac acgatgtccc agcggctcgg cgccccgggg
     1081 gatttcgcgc aggcctacgt catcgctcac gaggtgggtc accatgtgca gaacctgctc
     1141 ggcatcaccg gcaaggtcga tgcgatgcgc gggcgcgtca gcgaggccga acagaacgcg
     1201 atgagcgttc gcgtcgaact gcaggccgac tgctttgccg gcgtctgggc gcacgattcg
     1261 cagcaaagca agggctggct cgaaagcggc gatgtcgagg aggcgctcaa tgcggcgacg
     1321 cagatcggcg acgacaacct gcagcgccag tcgcgcggca ccgtggtgcc cgagagcttc
     1381 acccacggca ccagcgccca gcgcgtcaag tggtttcagc gcggcctgca ggggggcagc
     1441 ctgaagcagt gcaacacctt cgacaacaag gcgctgtagg cgcggccgat gcgggcggcg
     1501 taagtcggac ctcacaggag ccacggtgtc gaacattcag tacgcattca tcgagcgagg
     1561 acgcgtcccg agccggtcgg aactgcaggc atcgatagat gccttgggat tcgatctcag
     1621 gcttcctccc gagtacacgc ctttcgaaga ctcgggtttc ctgcccttca cgctgaatgg
     1681 tgaaacaggc cctggattcg aaattcagta cgacgcggca agcgagctga tcggcgagga
     1741 ccaggcgctg agggaaatgg ctgcaggtcg cgaccattgc gtcagcatgt catggcacgg
     1801 aagcatgaag gacctggctt gcgtcttgat cgtgagctgc gcactgatga aggatttcga
     1861 ttcagtcgtt tcgtatgaag gcgagccgcc cgagccgctg gagagtctgc tttcggtggt
     1921 gcccgaggtc ctggcggatg cgcgggcgca ggaagcaagg gatgcgaatc gaagcgcccg
     1981 acccgagaag aaaaagccgt ggtggaagct ctggtgaggt cctgcgttca gctcagttcg
     2041 aacagcaggc gccgcacgcg tttccaggct cctgcgtccc tttcctgaag attcccatgt
     2101 attcatcgac attcatcttt gccaaggggc agtgggacga cgagttccat cgactcgacg
     2161 aggaaatcgc ccaggccgcc aaggcgacgc ccggctacct gggcgaggag tcgtgggaga
     2221 accctgctca gggcctgatt tccaacgtgt actactggga gtctctcgag gccttgcagg
     2281 cgctgatgcg tcatcccgct cacctgcagg ccaaggcccg gcaagccgac tggctcgacg
     2341 gctatcaggt ggtcatctcg caggtgctgc gaacctacgg tgactccagg ctggcgggca
     2401 agctgcccac cgctggcgcg gcctagccca ttgcccgggc ggctcgcagc ggcagcgcgc
     2461 ttcctggcgg ctagcggtcg atgggcagcg gcttgccggc cttgtgcagg gccagccagc
     2521 gctgcaactc cgcgacgtgt tcggtttcct cgtcgacgaa ctcgcgggcg aagtggcgca
     2581 cttcggggtc ctgcgtcgat tcgaggatct cgcggtagta gtcgaggctg gcgctctccg
     2641 catccagcgc cacctgcagg gcctgctcgc gtccgatgaa cgggtcggcg gcccagatcg
     2701 ctgcggtctc ggggctctcg atgtcgggcc attcgaaatc gccagggcga aatgccggga
     2761 gctcgtggaa tccggaacgg tcccgcgcgg tgcgcatgtg caggcgcgag taatcggcca
     2821 ggcgtcggaa gagcttgccc acctcgcgat tgccggccga gatcatcgcg tcggccagtt
     2881 gatcgaagcg cagggccgct tcttcctcca gcttgatcga gtaggccagg aacagctcga
     2941 cagagtccat gtcgttcgtc gtcatcgtcg gttcttcttc catgtcagcc gaatcggcgc
     3001 caccgtcgcg acccggacac ggagcgtgac gaacgtcgat atctgcaact tcggggccac
     3061 cgagcgggcc acgccgaatt cacggcgctc acctgcgacg ccaggtcgcg gcgaccggct
     3121 tggggatgat ttcgcggtgc gacgcgcgtg tcggggtcgc tacaaaagcc acgcaacaga
     3181 cactgattca ggccaatttc atgtcgggaa accgtccact gtccttgatt cccgtgggca
     3241 acgcggttgt cgaaacgatg ggcacaggca ttgctcttcg agaccacccc atcttcaaaa
     3301 atcaggagcg cgctgtcatg gccaacgtaa cgttttcttc cgaagtgatg ccacgtgaca
     3361 tcaccgtgta tgcggtcgcg ggcgaccgcg gcaatctgct ggcgctggcc aaggaacacc
     3421 acattccgat tccgttcgac tgccaggacg gcgagtgcgg ctcctgtctc gtcgaggtca
     3481 agcacctgac gaagggcgtc cgctatggca tcgccctgac cgagaaggaa aaggaactgc
     3541 tcaagcagct cggcaagatc accaaggaac agttgatgga cgccgaggtc aacgacatgc
     3601 ctccgccgtt ccgcctggcc tgccagtgct tcgtgcgtga cgaggacacg atcgtcacgt
     3661 tcgagggcga caagacgctg ccggtcaagg gaccgacgct ttccatcgct gcggcggtct
     3721 acaagggtgg tgtccaggtt cacaccatgg atgacttcct cgcctacgcc gtcaaggtgg
     3781 aagaggatgc ggccgtgcac ttcgagcagt tggcgaaggc catggccgac tgcggcaaca
     3841 aggatgtggc cgcgctgttc gagcgtctgg gcagctactc gcgcctgcac ctcgaggaag
     3901 cgaaggccaa gtgcaagcgt cacgacgtca ccctgacgct gccgccgagc tcggcctggc
     3961 ccgacagcga aacgcctgag aagacgacgc tgtgggccgg cgatccgtcg ctgtcgcgtc
     4021 tcggcgccct gaaggtcgcc ctgcagggcg agcgtcgcgg cttcgagttc tattacgcca
     4081 ttgccggcac gacgaccgat gccgacatcc gctcggtggc gaaggagttc gtgcgcgagg
     4141 agaccgcgca cgtcgaggaa ctcaagcgct ggatcgagca ggaggagtcg gcattgcgca
     4201 tgaaggccga cgcttcggcc accgcctgac cccggggcct gtcaacgttg cgtgacgggc
     4261 cgcgccgctt ggccgcaggc cgcgaggcaa ctagcccagc acgaccggaa tcgtcagcac
     4321 ggcacgccgt ccgatctgcg cgttgacgcg ctgacggacg gcttcgacct tgtacggccg
     4381 cacgatcgcc gatcgtgctc ccagggcaag cacctgcgcg atgcccggat cacccgccgg
     4441 tccgcaggcg atcacgggca cgccgggcag cgcggcctgc aagcgggtca cgaccgcggt
     4501 gccttcggct tcgatgacgc tggcgcccag caggatcaca tcgagtgcga ggccctgtgc
     4561 gcggccgatg gcctcggcgc tgcaggtcac gacattgacc tcgagttcat cggccagcat
     4621 gaactgcagc gccatcgcgc tgatgtcatc gctgtcgatg atgaaggcgc gcttgctttc
     4681 cacggcacgc tgggtgtcga caccaatctg cattcgagtc tccctgagaa cgaggcggct
     4741 gccccgcgcc gtgagagcaa gcggcgtgcc ccgcgcgcat cgctcgaagc cgtcgcagcg
     4801 ctgtgtccgt acatgttgtg tttgtcggca tccgaacacc cggcaatctc tgcaagtgct
     4861 tgatttgact gggttcggca ttggcacgcg tctcgcaatg gaaggccatc caccacctgt
     4921 ttcgcgaggc ctgtcgatgt cactcaacac tgcttacgta gacgcttccg cccttggcaa
     4981 atcgcgacac gcggtcagcc agattctcga gaccgccgca tcgggtggtt gcgggaccac
     5041 gggtggcgag ggcaaggcca gctgcggcac atcggccggc cccgacgaca tgccgcagga
     5101 gatctgggac aaggtcaaga accatccgtg ctattcggaa gaggcgcatc accactatgc
     5161 gcgcatgcat gtggcggtgg ctcctgcctg caatatccag tgcaactact gcaatcgcaa
     5221 gtacgactgc agcaacgagt cacgccccgg tgtcgtgagc cagaagctca cgcccgagca
     5281 ggccgtcaag aaggtcgtcg cggttgccag cgagatcccc cagatgaccg tgctcggcat
     5341 cgccggaccg ggcgatgcgc tggccaatcc gaagaagacc ttcgacacct tccgcatgct
     5401 tcaggagcag gcacccgaca tcaagctgtg cctgtcgacc aacggcctgg ccctgc
//
LOCUS       DAOORV010000216         5386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18294_length_5386_cov_5.866066, whole genome shotgun sequence.
ACCESSION   DAOORV010000216 DAOORV010000000
VERSION     DAOORV010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18294_length_5386_cov_5.866066"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..1704)
                     /locus_tag="PKV56_17430"
     CDS             complement(436..1704)
                     /locus_tag="PKV56_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69638.1"
                     /translation="MTTAQVPSVYRRLSTAIGPEHWQGAVDKQEAAIRSNHFLRDYLR
                     SEYTIAYQLEKLRRLVVAHGDVPMQACNDRSIFPALGFAAQVLGILNTCTKKQARTFV
                     KRVRTAFCTAENMHGLRLELQAATHFTRRGKHVSWHRANNGGTFDLLVDDLGPSGLEV
                     ECKSISDNKGRRIHRLDALNFWGKVWTDISSVAQHLHTGMAVVLTVPYRLPAEPEQRT
                     ALAREVVSQIVAGTGARLSDGSDLRVQNFDPDPVKAAIEGDRRAMRTAIDVATGTTNR
                     EAALYGNPAGGMLAFVMQSAVEDDVLDQVFATLNDSASRQFTGKRGGLFWVALQGLDS
                     EQLMSVHEQDNDPGQRPTGLRLGVSNYLANAPDHIVGVVFGSRSGLHPTVDGNTDMGG
                     ATNFFLKDEGPFWHESFREPLRGSGGIAQA"
     gene            complement(1768..3216)
                     /locus_tag="PKV56_17435"
     CDS             complement(1768..3216)
                     /locus_tag="PKV56_17435"
                     /inference="COORDINATES: protein motif:HMM:NF039631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6035 family protein"
                     /protein_id="HOX69639.1"
                     /translation="MRPPSGPGPQQTVRLAIDSQTGELVPAEALLAMSEDEFSGLRRE
                     AMEARVDRSKGGEAVRYQCAICKFPLYLSRRISGAQNRWFVHDGKSHECPWYEGNRLA
                     PEQIKALVYRGQQEGREHRELKEYIAHWLSRDPLVSDVNSEQTTFSEVVKGEWRRPDV
                     KCLYGGVKLVFEIQLSYTFLSDVIARDGFYRREKTFVIWVFSKFDRSRAAVTDEAFFN
                     RRNLFVIDADARRHTGDRSALTFSGYHQVPTLDDNWRWRDTWQSMPIGMADLKLPSDT
                     WRPYFFDYEARRKQIETERIETSRAEQARQWATGIAAYREAALRYFASDHGEKERRAL
                     VAVVDELEEHTAWHPGFEGLRASRFYGYHGVLAVLLSIQTGKPVSYSPQLSVFQVIEA
                     GLRTASRVGLHAYAILYLWAYKTYRPAVPEKQRKWIIDYARKIKLSFARGESVYRRDT
                     SFDDVIDVLFPELGEHLSNPFGTDLMDDESDG"
     gene            3350..4927
                     /locus_tag="PKV56_17440"
     CDS             3350..4927
                     /locus_tag="PKV56_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69640.1"
                     /translation="MNWARQGAHRNAHFNSRYCMNSPLLQDFPAGRWDEMCVRLAPLL
                     GDVKGALGPQWHEKAEAFFTEADFTVADAILSGTKSLPDWFIIRYAPAGQHLDDEGDT
                     VACAPSWSDGLAVLLHACESNRSLPSRVECDGAARTLRLFVGEELLATLLPLPAAYVS
                     PDLRKRALSTITGLRKAADTDAELLKRARRWPGLSALGAVESRAAYEMQEFRERMAHH
                     PIPLEWLQSARMLCAACSKPEPDTATVQAVAAAALGATSWNHLASPHREFSARLLQPW
                     YVSKDDEICAFHADAIDAVADLFTRGTKVWAAGWASFALESHYGITAPDYVPIYTLRE
                     PSQGTARRSFDERQVAVYPVSRSEAKAEVLARVAGVKPESTDAIAALFGIGLPVDVKA
                     RMLDERSDEILIGQDGPWRFTRSGDPLDQHTSIWVHRVGIDGNSLWCAAVPAYKGLLQ
                     THRETGFYVFCADYDGAHPVAVIDGLSPATVAQVRVNLRDTTEDRMEFREESRSARDR
                     EDFRKLLGHALSRRSVA"
     gene            complement(5149..>5386)
                     /locus_tag="PKV56_17445"
     CDS             complement(5149..>5386)
                     /locus_tag="PKV56_17445"
                     /inference="COORDINATES: protein motif:HMM:NF021209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="HOX69641.1"
                     /translation="LIGSRRVAVQIDPEAFDRYGITRTPTFVLDMDGTGAEACASRVC
                     SSSKQFAKVAGDVTLDYAMRYLNQGAGESARPKQ"
BASE COUNT          941 a         1707 c         1691 g         1047 t
ORIGIN      
        1 cgcgtgccac ggggttcgcg gcctggccgg cggcaacacc ttcctcgtgc gggccagcca
       61 cggcggcggt ctgcgttgtc aggaagcagg cggcggtgat gcatgcgacg aggcggcgcc
      121 acggcgaggc gcggtgcgcc gcagaaggcg gcggggcggg acggtggcta tcggggcctg
      181 gcggcgtgga gtgaggcacg cccaatcgtc aggggcggta cggctggtgt cgcggggaaa
      241 cggtgggtca gagagtgggg tttcgaagct gggcatgcga atggccgaat tgctattccg
      301 cggcgaatga cgctgttggc cgccagcgct cccacttgcg ttgccggacg gcaagccgtc
      361 gttagacgtc gccgcggcga tgaaacctct agctaggcct gtcaagcggt ccgcccgccc
      421 gaggtggacc gttttctagg cctgggcgat gccgccggat cccctgagag gctctctgaa
      481 gctttcgtgc cagaacgggc cctcgtcctt caagaagaag tttgtcgcgc cgcccatgtc
      541 cgtattgccg tccaccgttg ggtgaagccc gctccgactg ccgaagacga ctccgacaat
      601 gtgatcgggg gcgttggcca ggtagttgct gacgccgagg cgcagtccgg tcggacgctg
      661 gccaggatcg ttgtcctgct cgtgaaccga catcaactgc tctgagtcca gcccttgcag
      721 cgcaacccag aacaagccac cgcgcttccc cgtgaactgc ctggaagccg agtcgttgag
      781 ggtggcgaat acctgatcca gaacatcgtc ctcgacagcg ctttgcatca cgaaggccag
      841 catccctcca gccgggtttc catacagcgc tgcttcccgg ttggtcgtgc cggtcgccac
      901 atcgatggcc gtgcgcatgg cccgacgatc gccttcgatt gcagccttga ccgggtccgg
      961 atcgaagttc tgcacacgca gatcggatcc gtcgctcagc ctggcaccgg tgccagccac
     1021 aatttgggag acaacttccc gcgccagcgc agtccgctgc tcaggttcgg caggtagccg
     1081 atacggcacc gtcaacacga ccgccatgcc ggtatgtagg tgctgcgcga ccgaggatat
     1141 gtccgtccaa acctttcccc agaaattcag cgcatccagt cgatggatgc gccggccctt
     1201 gttgtcggag atcgacttgc actcgacctc aagccctgac ggtccaaggt catcgaccag
     1261 gagatcgaac gtgccgccgt tgttcgccct gtgccatgac acgtgcttgc ctcggcgcgt
     1321 gaagtgcgtt gccgcctgca actccaggcg cagcccatgc atgttctctg cggtgcagaa
     1381 ggccgtcctg acgcgcttca cgaacgtgcg cgcctgcttc ttcgtgcagg tgttcaggat
     1441 tcccagaacc tgtgccgcga agcccaaggc agggaagatg gatcggtcgt tgcaggcctg
     1501 catcggtacg tcgccatgcg cgacaaccag gcgccgcagc ttttccagtt ggtacgcgat
     1561 ggtgtactcg gagcgcaggt agtcgcgaag gaagtggttc gaccggatcg cggcttcttg
     1621 cttgtccacc gccccttgcc agtgctccgg cccaatcgcg gtcgaaaggc gccgatacac
     1681 agacggcacc tgcgccgtgg tcatctcagt tgccagcact tcagttcaac ggccttcttt
     1741 ggtggtttga cggaccgacc gttcatccta cccgtcgctt tcgtcatcca tcaggtcggt
     1801 accgaatggg ttcgacagat gctccccgag ctccgggaac agcacgtcga tcacgtcatc
     1861 gaacgaggtg tcacggcggt agaccgattc acccctggcg aatgacagtt tgatcttgcg
     1921 cgcgtagtcg atgatccact tgcgttgctt ctcaggcacg gccggcctgt acgtcttgta
     1981 ggcccagagg tacaagatcg cataggcatg cagtcccact cggctggcgg ttcgaagacc
     2041 agcctcgatg acctgaaaca ccgacaactg cgggctgtaa ctgaccggct ttccagtctg
     2101 gatcgatagc agcaccgcca ggacgccgtg gtatccgtag aaccgtgacg cgcgcaggcc
     2161 ttcgaagcca ggatgccacg ctgtgtgctc ctccaactcg tcgacaaccg ccacgagcgc
     2221 tcggcgctct ttttctccgt gatcgctcgc gaagtagcgc agtgccgctt ccctgtacgc
     2281 agcaatacca gttgcccatt gccttgcctg ttctgcgcgc gatgtctcga ttcgttcggt
     2341 ctcgatctgt ttgcgtcgag cttcgtagtc gaagaagtag ggccgccacg tgtcgctggg
     2401 tagcttcaag tcggccatgc cgataggcat ggactgccag gtgtcgcgcc aacgccagtt
     2461 gtcatccagg gtcggcacct ggtgatagcc gctgaacgtc agtgccgatc ggtcgcccgt
     2521 gtgtcgtctt gcatccgcgt cgatcacaaa caggttgcgc cggttgaaga acgcctcatc
     2581 ggtgaccgcc gcacggctgc gatcgaactt cgagaacacc cagatcacga aggtcttctc
     2641 tcgacggtag aacccatcgc gtgcgatcac gtccgacagg aacgtgtacg acagctggat
     2701 ctcaaagacc agcttcacgc cgccgtacag gcacttcacg tccggccgtc gccactcgcc
     2761 tttgaccacc tcgctgaacg ttgtttgctc ggagttcacg tccgacacca agggatcgcg
     2821 gctgagccag tgcgcgatgt actccttcag ttcacgatgc tcgcgtcctt cctgctgacc
     2881 tcggtacacg agggccttga tctgctccgg cgccaggcga ttgccctcgt accaagggca
     2941 ttcgtgtgac ttgccgtcgt gcacgaacca gcggttctgt gccccgctta tgcgccgcga
     3001 gaggtacagg gggaacttgc agatcgcgca ctggtagcgg acggcctcgc cacctttgga
     3061 ccgatcgaca cgggcctcca tcgcctctcg ccgcaatccg ctgaattcgt cttccgacat
     3121 ggccaacagc gcctcagccg gaacgagctc accggtctgg ctgtcgatgg ccagcctgac
     3181 ggtctgctgc ggtcctgggc ccgatggcgg tcgcatggct tcatgcacca gagcttgcct
     3241 ggcgttcctc ccgtgagcta ccattctctt cggctgcccg agcctgccgt gcacccgcac
     3301 tctgcggaag gtaaaccagg gtcactgact tcctcccgct tggtacctcg tgaactgggc
     3361 gcggcaaggt gcacaccgca atgcccactt caactcgagg tattgcatga actccccgct
     3421 tcttcaggac ttcccggccg gccgctggga cgagatgtgc gttcgtcttg cgccgctgct
     3481 cggcgacgtc aaaggcgcct tggggccgca gtggcacgag aaggccgaag ccttcttcac
     3541 cgaggccgac ttcacggttg ccgatgccat tctcagcggc accaagtccc ttccggactg
     3601 gttcatcatc cggtacgccc ctgctgggca acatctggac gatgagggag atacggtcgc
     3661 ctgcgctcca tcctggtctg atgggctggc ggtcttgcta catgcctgcg agagcaatcg
     3721 cagtctgcca agccgggtgg agtgtgacgg ggcagcgcgt acgctgcggc tcttcgtcgg
     3781 cgaggaactg ctcgccacct tgctgccgct tccggcagcc tacgtctcgc cggacctccg
     3841 caagcgtgca ttgagcacca tcacgggtct caggaaggca gctgacacgg atgccgagtt
     3901 gctcaaaaga gcgcgcaggt ggcccggcct gtcggcgctt ggagcggttg aatctcgcgc
     3961 cgcctacgag atgcaggagt tccgtgaacg gatggcccat catccgatcc cacttgagtg
     4021 gctgcagtca gccaggatgc tgtgtgcggc ttgctccaag cccgaacccg acaccgcgac
     4081 agtgcaggct gtcgctgccg cggcgctggg cgccacgagt tggaatcacc ttgcgtctcc
     4141 gcacagggag ttcagtgcgc gccttttgca accctggtac gtcagcaagg atgacgagat
     4201 ctgcgcgttt catgccgacg ccatcgatgc cgtcgctgac ctgttcactc gaggcaccaa
     4261 ggtctgggcg gcgggctggg cgagcttcgc gctcgaaagt cactacggca tcacggcgcc
     4321 ggactacgtt cccatctaca cgttgcgcga gccgtcgcag ggcacggcgc gacggtcgtt
     4381 cgatgaacga caggttgcgg tctatccggt gagtcggtct gaagccaagg cggaggtctt
     4441 ggcgcgtgtc gccggcgtga agccggaatc aaccgatgcg atcgctgcac tgttcggaat
     4501 tgggctgcct gtagacgtca aagcgaggat gctcgacgag cgatcagacg agatcctgat
     4561 cgggcaggac gggccgtggc ggttcaccag gagcggggat ccgctcgacc agcacacttc
     4621 gatctgggtt caccgagtgg gcatcgacgg caacagcctt tggtgcgcgg cggttcccgc
     4681 ctacaagggg ctgctgcaga cgcatcgcga gaccgggttc tacgtcttct gcgccgacta
     4741 tgacggagcc caccccgttg ccgtcattga cggattgagc cccgcgactg tcgctcaagt
     4801 cagagttaac ttacgagata cgacagagga tcgaatggag ttccgggagg agagccgaag
     4861 cgcccgtgac agggaggact tcaggaaact cctcggtcat gccttgtcca ggcgctcggt
     4921 ggcttgagaa gcctgcttcc ttgtagtaga agaagcgcga catggcggcc acaaggccgg
     4981 cgcggattca tgtcgaggcc agggcagccg gcaaagcgag ccgccccggc agcgccagaa
     5041 cggctaggta cggcggtcgt ccaggctggc tgcggcgaat tggctgacag cactcagtct
     5101 tcttcggagg cggctggtct tgggcgcgag gcggaatggg agcggtgcct actgctttgg
     5161 acgcgctgat tctccggcgc cctgattcag gtagcgcatc gcatagtcca gggtcacgtc
     5221 tccagcgacc ttcgcgaact gcttcgatga gctacacacg cgtgatgcgc atgcctcggc
     5281 cccggtgccg tccatatcca ggacgaatgt cggagtcctc gtgattccat agcgatcgaa
     5341 cgcttccgga tcgatctgaa cagcaacacg gcggctgccg atcagc
//
LOCUS       DAOORV010000217         5361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18411_length_5361_cov_9.196193, whole genome shotgun sequence.
ACCESSION   DAOORV010000217 DAOORV010000000
VERSION     DAOORV010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18411_length_5361_cov_9.196193"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKV56_17450"
     CDS             complement(<1..206)
                     /locus_tag="PKV56_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HOX69642.1"
                     /translation="MNPDAATLWRRPRWALAALLACLGMVGPFSIDTYLPAFSGIASS
                     LNASPVQMQQTLSAYLFGFAVMNL"
     gene            complement(266..609)
                     /gene="rnpB"
                     /locus_tag="PKV56_17455"
     ncRNA           complement(266..609)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV56_17455"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(651..1526)
                     /gene="htpX"
                     /locus_tag="PKV56_17460"
     CDS             complement(651..1526)
                     /gene="htpX"
                     /locus_tag="PKV56_17460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOX69643.1"
                     /translation="MKRIALFLLTNVAVMVVLGIAVNLLGLNRYLTANGLDLGSLLGF
                     SLVMGFGGAIISLLISKPMAKWTTGAQVINDSRDATHQWLVSSVARFAERAGIAMPEV
                     AIYDGAPNAFATGAFKNSALVAVSTGLVQSMTREEVEAVIGHEVAHIANGDMVTMTLI
                     QGVMNTFVVFLSRVIGYVVDRMILRNDREGPGVGYMITTVVMDLLLGVLAAMIVAWFS
                     RQREFRADAGSAQLLGRKQPMINALARLGGLDPGEMPKAMQAMGISSRPSGLMALFST
                     HPPMEERIARLRQIP"
     gene            complement(1554..2354)
                     /locus_tag="PKV56_17465"
     CDS             complement(1554..2354)
                     /locus_tag="PKV56_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HOX69644.1"
                     /translation="MTEVAGGRSVWSTIDWAAPWLAPHRSVGEPIGRLVESGASVVDA
                     LNQALGRRSSRAVAKPRCFVGQQALAPGEPYESFIGRTGSVPTRENAHDLFNGLAWLI
                     HPALKARLNALHIEQIEHGAVGAVRGPVRDALTLFDENAAWLQAPPALIDALRQRDWA
                     ALFVTHRVLWSHARLELFGHALIEKLMRPRKPITAHVWVVPFEVADPAAWLAGHLGAE
                     VLASRPWLPLPVLGVSGWWNLNEHPGFYDDATVFRRSARALKSGGSTA"
     gene            complement(2347..3258)
                     /locus_tag="PKV56_17470"
     CDS             complement(2347..3258)
                     /locus_tag="PKV56_17470"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOX69645.1"
                     /translation="MDSITRIALLIDADNVSVEVLRQVLHKLGMSGARLQHRRAYGSV
                     QKAAEFAEICRDHAIRFLPSTFAGPNATDIAMAIDAVELVLRQPVDEVVIVSSDMDYS
                     PLIVRLRELGCRVTGYGQQGKSGRDIERDYERVYDQFEVIGPAKARPAVRATTRAAAP
                     PEVTAPAAPARARARRPAARVAIPVPLTAAVEAPAESPAARPTAPRRRPAAKTPPAAQ
                     ALLPAAAVPRLLPAAVEAVLEACPDLRGGAAVRLNGVAVALRERGLLTKSGKTTSFFK
                     KLDSHFALLPIEKPESVRFLGAAPSGD"
     gene            complement(3272..4318)
                     /gene="pyrC"
                     /locus_tag="PKV56_17475"
     CDS             complement(3272..4318)
                     /gene="pyrC"
                     /locus_tag="PKV56_17475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOX69646.1"
                     /translation="MQNRTMNSLTLTRPDDWHLHLRDGAAMASVLPDTARQFARAIVM
                     PNLKPPVTTTAQALAYAERIRQALPRGLVFEPLMTLYLTDQLPPDEILRAREAGIVAV
                     KLYPAGATTNSDAGVTDLRKTHATLDAMQRLGMPLLLHGEVTDADIDLFDREAVFIDT
                     RLIPLRRDFPELKIVFEHITTREAAQYVASAGAHTAATVTAHHLLYNRNALFMGGVRP
                     HYYCLPVLKREVHRQALVAAATSGSDRFFLGTDSAPHATHLKEHATGCAGCYTALSAI
                     ELYAEAFDAAGALDRLEAFASFHGPAFYGLPRNAGTITLRREAWTLPESLPFGETELK
                     PLRGGEVLPWRLAA"
     gene            complement(4440..5174)
                     /locus_tag="PKV56_17480"
     CDS             complement(4440..5174)
                     /locus_tag="PKV56_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOX69647.1"
                     /translation="MIDIKNISKWYGSFQVLTDCTTSINKGEVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGDIIVDGISIADPKTDLPKLRARVGMVFQHFELFPHLTVTENLTLAQ
                     IRVLKRNPEDAKSRGLKMLDRVGLMVHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVQLANEGMTMMCVTHEMGFARKVSHRVIFMDAGRIIEDC
                     TREGFFGQPEQRSPRAKEFLSKILQH"
     gene            complement(5219..>5361)
                     /locus_tag="PKV56_17485"
     CDS             complement(5219..>5361)
                     /locus_tag="PKV56_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOX69648.1"
                     /translation="KGFEVAGKNFNRPVETYLVAAVVYFVICFSLSMLVRRLQKKISI
                     IR"
BASE COUNT          914 a         1786 c         1794 g          867 t
ORIGIN      
        1 aacaggttca tcaccgcgaa gccgaacagg taggccgaca gcgtctgctg catctgcact
       61 ggcgacgcat tcagcgatga ggcgatgccg ctgaaggccg gcaggtaggt atcgatggaa
      121 aacgggccga ccatgcccag gcacgccagc agtgccgcca gcgcccagcg cggccggcgc
      181 cagagcgtgg cagcgtcagg gttcatcggg tccgttcgtc cggttcgtcg agggcaggcc
      241 gcaccctgcg accgccaaaa aattagtgtg aagcgagccg ataggccgga ttctgtgcag
      301 ccgtgccatc ttgcgatcgc ccggccgtga ccgccattcc tctgggccgt gcatcactgc
      361 acggctcggt gccacctacc cgccagctcc gcggagccac atcaacgctg gcctatttgg
      421 tgttgctgcg cgcagagatt gctcgtttca cccgaagcgg ggcttgcgcc ccacctcgac
      481 tcgtctctgt agctctgatc ctcacctcgc ggtggacagc cgttagctgc tgcgctgccc
      541 tgtgcagtcc ggaccttcct ccagtgcgcc ctttcgggac cctgcaccag cggcggtctg
      601 gcttgcttca cctctggatt atctcagggc ggccgcgggt tgcgcgcggg tcagggtatc
      661 tgacgcaggc gcgcaatgcg ctcctccatc ggcggatggg tcgagaacag cgccatcaga
      721 ccgctcgggc gcgagctgat gcccatcgcc tgcattgcct tcggcatctc gccagggtcg
      781 agcccgccaa gtcgcgccag cgcgttgatc atcggttgct tgcgaccgag caactgggcc
      841 gagccggcgt cggcgcggaa ctcgcgctgc cgcgagaacc acgcgacgat cattgccgcc
      901 agcacgccga gcagcaggtc catcaccacc gtggtgatca tgtagcccac gcccgggcct
      961 tcccggtcgt tgcgcaggat catgcggtcg accacgtagc cgatgacgcg cgacagaaac
     1021 accacgaagg tgttcatgac gccctggatc agcgtcatcg tcaccatgtc gccgttggca
     1081 atgtgcgcaa cctcgtgccc gatcaccgcc tcgacttcct cgcgcgtcat cgactgcacc
     1141 agcccggtgg acaccgcaac cagcgccgag ttcttgaagg caccggtggc aaacgcgttg
     1201 ggtgcgccgt catagatcgc cacctcgggc atcgcgatgc ccgcgcgctc ggcaaagcgg
     1261 gcgacgctgc tgacgagcca ctggtgcgtg gcatcgcggg aatcgttgat gacctgggcg
     1321 cccgtggtcc acttggccat cggtttgctg atcagcagcg aaatgatcgc gccgccgaag
     1381 cccatcacca gcgagaagcc cagcaatgag ccgaggtcga ggccgttggc cgtgaggtag
     1441 cggttcaggc ccagcagatt cacggcgatg cccagcacca ccatcacggc gacgttggtc
     1501 agcaggaaca gggcgatgcg cttcatggga tcctttcagc agtcgagcag cggttatgcg
     1561 gtcgacccac cggatttcaa ggcgcgggcc gaacgacgga agaccgtcgc atcgtcatag
     1621 aagcccggat gctcgttcaa gttccaccac ccggacacgc cgagcaccgg caacggcagc
     1681 cagggcctgg aggccagcac ctcggcaccc agatgacctg caagccaggc cgccggatcg
     1741 gcgacctcga acggaaccac ccagacgtgg gcggtgatcg gcttgcgcgg ccgcatcagc
     1801 ttctcgatca gcgcgtggcc gaacagttcc aggcgggcgt ggctccacag cacgcggtgc
     1861 gtgacgaaca gggccgccca gtcgcgctgc cgcagcgcgt cgatcagcgc aggcggcgcc
     1921 tgcagccagg cggcgttctc gtcgaacagg gtcagcgcat cgcggaccgg gccgcgaacc
     1981 gcacccacgg cgccatgttc gatctgttcg atgtgcaagg cattgagccg cgccttcaga
     2041 gcgggatgaa tgagccacgc cagtccgttg aacagatcgt gcgcgttctc gcgcgtgggc
     2101 acgctgccgg tgcgaccgat gaaggattcg tagggttcac cgggggcgag cgcctgctgc
     2161 ccgacgaagc agcgcggctt cgcgacagcc ctcgaggagc gccggcccag agcctgattc
     2221 agtgcatcga cgacggacgc tccggactcg acaagccggc cgatcggctc gccgacgctt
     2281 cgatgcggtg ccagccaagg cgcggcccag tcaatcgtcg accagacact ccggccgccc
     2341 gcgacttcag tcacccgagg gggcagcgcc caggaagcga accgattcgg gcttctcgat
     2401 cggcaacaag gcgaaatgcg aatccagctt cttgaagaac gacgtggtct tgccgctctt
     2461 ggtcagcagg cctcgctcgc gcagggccac ggccacgcca ttgagccgga cggccgctcc
     2521 gccgcgcagg tcgggacagg cttcgagcac cgcctcgaca gcagcgggaa gtaagcgcgg
     2581 cacggctgcc gccggcagca aggcctgcgc cgccggcggg gtcttggccg ccggccgcct
     2641 tcgtggcgcg gtgggacgcg ccgcaggtga ctcggcagga gcctcgacgg cagcggtgag
     2701 gggcaccggg atcgccacgc gcgcggcagg ccggcgcgca cgcgccctcg ccggcgcggc
     2761 aggcgctgtg acctccgggg gagcggccgc ccgggttgtc gcacgcacgg caggccgcgc
     2821 cttggccggg ccgatcacct cgaactgatc gtagacgcgc tcgtagtcgc gttcgatgtc
     2881 gcggcccgac ttcccctgct gtccgtagcc ggtgacgcgg caaccgagtt cccgcagccg
     2941 cacgatcagc ggcgagtagt ccatgtccga cgacacgatc acaacctcgt cgaccggctg
     3001 ccgcagcacc agctcgaccg cgtcgatcgc catcgcgatg tcggtggcat tcgggccggc
     3061 aaaggtgctc ggcaggaagc ggatggcgtg atcgcggcag atttcagcga actcggccgc
     3121 cttctgcacg ctgccgtagg cgcgccggtg ctggagccgc gcgccggaca tgccgagctt
     3181 gtgcagcacc tgccgaagga cctcgacgga aacgttgtct gcgtcgatca gcaacgcgat
     3241 gcgggtgatg gagtccatga gatttcccgg ttcaggcggc aaggcgccag ggcagcactt
     3301 cgccgccacg caagggtttg agctcggtct cgccgaaagg cagcgattca ggcagggtcc
     3361 acgcctcgcg gcgcagcgtg atggtgccgg cgttgcgcgg caggccgtag aacgccgggc
     3421 cgtggaagct ggcgaaggct tcgagccgat cgagcgcacc cgctgcatcg aaggcctcgg
     3481 catacagctc gatcgccgac agcgcggtgt agcagccggc gcaaccggtg gcgtgctcct
     3541 tgaggtgcgt cgcgtgcggc gcactgtcgg tgccgagaaa gaatcgatcg ctgccggagg
     3601 tggctgcagc caccagcgcc tgtcgatgca cctcgcgctt gagcaccggc aggcagtagt
     3661 agtgcggccg cacgccgccc atgaacagtg cgttgcggtt gtagagcagg tggtgcgccg
     3721 tcacggttgc tgccgtgtgg gcgccagccg aggcgacgta ctgtgccgcc tcgcgcgtgg
     3781 tgatgtgctc gaagacgatc ttcagttcgg gaaagtcgcg ccgcagcgga atcagtctcg
     3841 tgtcgatgaa aaccgcctcg cggtcgaaca ggtcgatgtc ggcgtcggtc acctcgccat
     3901 gcagcagcag cggcatgccg aggcgctgca tcgcgtccag cgtcgcatgc gtcttgcgca
     3961 gatcggtgac accggcatcg ctgttggtgg tcgcacccgc cggatacagc ttgacggcaa
     4021 cgatgccggc ttcacgggct cgcaggatct cgtcgggggg caactggtcg gtcaggtaga
     4081 gcgtcatcag cggctcgaag accagcccgc gaggcaaggc ctgacgaatg cgctcggcgt
     4141 acgccagcgc ctgcgcggtg gtcgtcaccg gcggcttcag gttgggcatc acgatcgcgc
     4201 gggcaaactg ccgtgcggtg tcgggcagca ccgatgccat cgcggcgcca tcgcgcagat
     4261 gcaggtgcca gtcgtcggga cgcgtgagag tcagcgaatt catggtgcga ttttgcatcg
     4321 cgccttgcgc ggatcgatcc gcacacggaa cacggcccgc cgggtcgcac gccgagccac
     4381 cccagcctgc ggttcccctc gcaagccaag aggaactggg gccgcgaccg gcgccgcaat
     4441 cagtgctgca ggatcttgga gaggaactcc ttggctcgcg gcgaccgctg ttctggctgg
     4501 ccgaagaacc cctcgcgcgt gcagtcctcg atgatccttc cggcgtccat gaagatcacc
     4561 cggtggctga ccttcctggc aaagcccatc tcgtgggtca cgcacatcat cgtcatgccc
     4621 tcgttggcca gttgcaccat cacatcgagc acctcgccga ccatctcggg atcgagcgca
     4681 gaggtcggct cgtcgaacag catcacgatc gggtccatgc tcagcgcgcg cgcgatcgcc
     4741 acgcgctgct gctggccgcc ggagagctgc cccgggaact tgtccttgtg caccatcagg
     4801 ccgacgcgat cgagcatctt caggccgcgc gacttcgcgt cttcagggtt gcgcttgagc
     4861 acgcggatct gcgcgagcgt caggttctcg gtcaccgtca ggtgaggaaa cagctcgaaa
     4921 tgctggaaca ccattccgac acgcgcgcgc agcttgggca gatcggtctt tggatcggcg
     4981 atgctgatgc cgtcgacgat gatgtcgccc ttctggaaag gctcgagcgc gttgacggtc
     5041 ttgatcagcg tgctcttgcc cgacccggac ggcccgcaca ccacgacgac ttcgcccttg
     5101 ttgatcgagg tggtgcagtc cgtcagcacc tggaagctgc cgtaccactt ggagatgttc
     5161 ttgatatcga tcatggtggc tccggggctt cgactcgctc ggcccgaaag gtgagggctc
     5221 agcggatgat ggaaatcttc ttctgcaggc ggcgcaccag catcgacagg ctgaagcaga
     5281 tgacgaagta gacgacggcg gcgaccaggt aggtctcgac cggccggttg aagttcttgc
     5341 ccgccacctc gaagcccttg a
//
LOCUS       DAOORV010000218         5353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18443_length_5353_cov_9.541903, whole genome shotgun sequence.
ACCESSION   DAOORV010000218 DAOORV010000000
VERSION     DAOORV010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18443_length_5353_cov_9.541903"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="traF"
                     /locus_tag="PKV56_17490"
     CDS             complement(<1..737)
                     /gene="traF"
                     /locus_tag="PKV56_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HOX69649.1"
                     /translation="MKRILVSLSLLSTVILPLTAEAQATASRYWSDAWRGWHFYEEPE
                     PEPEDRPSPPPKVAPPASRPTAGKPPEIVEFERLQKSLEETRNIAIMRPTEANVRRYM
                     ELEAKVVARASTFADVAQRIAWATPELDPTLHGRPVNAKALEVFDQLQMSQRSESIGA
                     LGRDHVLFFFFRSDCPYCHAFAPTLEAFQARHGIRVVAISVDGGPMPGFPDARRDNGI
                     ATTLKVTQVPAVYLAQPFTGKITPIGFG"
     gene            complement(748..1122)
                     /locus_tag="PKV56_17495"
     CDS             complement(748..1122)
                     /locus_tag="PKV56_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69650.1"
                     /translation="MKPHPVLALAMVIGVGSGAMAQDRTPVQDAKLLLIAAIDAPSGQ
                     AQGVLVGEIADAITQRFKGTTPIYIDVTTERRYAQAGCSRLKVRFWQDGVQLPGVPAP
                     RRQTIDFGINYCRDGQQPKSLS"
     gene            complement(1119..3518)
                     /gene="traN"
                     /locus_tag="PKV56_17500"
     CDS             complement(1119..3518)
                     /gene="traN"
                     /locus_tag="PKV56_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HOX69651.1"
                     /translation="MRLRKVVVWFTLVCFVTTQTASVAGPHEEGVAAGQAANPVTRGS
                     VTAPSATGVVPGYTTTPPERSYYRQPNLASQGNARLALCATLPNDPTCQAQRGALASA
                     NTPRPAVSPDDPAVAAARDIGRSPSSVLGSLAAYYSGCTTTVTNQPATTQTRSCLRYL
                     GVGNYSCARSLTVATDRTTSCTPGDWFAHAGSGRAGIDVQCLPDRPETAQHFRVTQDS
                     NPLTFFDVNMTTPVVFPEMVAVLETNYSGMTGLPIRTGVWVADKSCAGSTCSLTAMIA
                     AEVQEICTGNGDSGFSCTSAEPFLKIYAACRAGTQSGDNIQDTLCQGDNGCTTTVLDG
                     AKCYAPAGGWTPYAGTDITGTVPGYYWNIDADRAVVGWAPNPAYGPIPTMRLTYTRAT
                     TAVTTTDRWDDQCPALAAGGRCTVSTAAVCTDGPATKVIDGISVYRDCWEYTSTMTCS
                     SGAPVDQCAPLVAAGCTPQSSACKQTNATTGVCEVVEDGYSCPVPAQSVTTASNCPSN
                     VFCLNGNCFDISAPNDADFARSMSMLEAGREAGVYLDSDRMQVFKGEENRCRDRLLKN
                     CCYADAAGAGMTNQSLFGSGSRLVYDVLMNSENREFLYQGMSALLSGAGFSGSFTSYG
                     VTVAVNGTALPAGSSVLYAGDSLVVAFDPWSLVIAVVIYIILSMMSCNEDEGKLAMKE
                     GAKLCHTIGTWCSSCIRILGACVSCIEHTTSKCCFNSMLARIVNEQGRAQIGKGWGGA
                     QSADCSGFTVAQLQTLNFAAMDLSEFYASLVPMLPDVATLQGNGASRIPTCYYGQGRC
                     Q"
     gene            complement(3515..4195)
                     /gene="trbC"
                     /locus_tag="PKV56_17505"
     CDS             complement(3515..4195)
                     /gene="trbC"
                     /locus_tag="PKV56_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HOX69652.1"
                     /translation="MLCAAAALVLQSAALGQTLPKVTDADIERARQQHPMPTDAELAR
                     VPVPSTPRIDALPQPATRTPVDLEALAKGFDAQADKPVLGAASGPSLLVFVSFAMPEA
                     TITRLLDQASRARATLVLRGLVNGSLRDTVERMQRLIGNRQVAVQIDPQAFDRFSIVR
                     TPSFVLVREGAASKPCAAGMCIASDQFVLAAGDVSLDYVLRFFERSAPGMARDASSYL
                     QRMKGAVR"
     gene            complement(4230..5234)
                     /gene="traU"
                     /locus_tag="PKV56_17510"
     CDS             complement(4230..5234)
                     /gene="traU"
                     /locus_tag="PKV56_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HOX69653.1"
                     /translation="MLATLLALTTGLLPALSHAADSLTCTGRFPNPITEICWSCILPI
                     SIGSATIANVDGQEDIANPSNPVCSCGVNPTIGLSIGFWEPARHVEAVRKPFCLASLG
                     GIDLDPGISAPAAARFTRAEGDGDGGSFYQAHFYVNPVLYWLEVVTDFPCLERGSIDL
                     AYLTEVDPLWNDDELTLILNPEAVLFANPIAVAACAADCVAATAGFGIAEMFWCAGCQ
                     GGIYPFDGHVPYHMGGVRTAALIAQRLTAKMHRELLAWGWHGKPGLCGPYFLPAMDKT
                     AYKTQLTYPVANTAKDGGRCCQPFGRSTIVWGAGKEYPVRGEDFAFMLFRKRNCCVGY
                     "
BASE COUNT          824 a         1746 c         1926 g          857 t
ORIGIN      
        1 ccgaagccga tgggcgtgat ctttccggtg aagggctggg cgaggtagac ggccgggacc
       61 tgggtgacct tcagcgtggt cgcaatgccg ttgtcgcgcc gtgcgtccgg gaagccgggc
      121 atcggcccac catcgacgct gatcgccacc acgcggatgc cgtggcgtgc ctggaacgct
      181 tccagcgtcg gcgcgaaggc atggcaatag ggacagtcgc tgcggaagaa gaagaacagc
      241 acatggtcgc gtcccagcgc accgatcgac tcgctgcgct gggacatctg cagctggtcg
      301 aagacctcca atgccttggc gttgacgggg cggccgtgca gggtcggatc cagctcgggc
      361 gtggcccagg cgatgcgctg ggcgacgtcg gcgaaggtgg aggcgcgcgc caccaccttg
      421 gcctcgagct ccatgtagcg acgcacgttc gcttcggtcg gccgcatgat ggcgatgttc
      481 cgggtctcct cgagcgactt ctgcaggcgc tcgaactcga cgatctcggg cggcttgccg
      541 gcagtgggcc tcgaagcggg aggggcgacc ttgggtggcg gagagggtcg gtcctcgggc
      601 tccggttcgg gttcttcgta gaaatgccag ccgcgccacg catcggacca gtagcgcgac
      661 gccgtggcct gcgcctcggc ggtcaacggc aagatgacag tcgacagcaa cgacagcgaa
      721 acgaggatcc gcttcatcac cggcccctca cgacaacgac ttgggctgtt gcccgtcacg
      781 gcagtagttg atgccgaagt cgatggtctg ccgacgcggt gccggcacgc cgggcagctg
      841 gacaccgtcc tgccagaagc gcactttcag gcgactgcag cctgcctggg cataacgccg
      901 ctcggtggtc acgtcgatgt agatcggtgt ggtgcccttg aagcgctgcg tgatcgcgtc
      961 ggcgatctca cccaccagaa cgccctgggc ctgcccgctc ggcgcatcga tggcggcgat
     1021 cagcagcagc ttggcgtcct gcaccggggt gcggtcctgc gccatggctc ccgagccgac
     1081 gccgatgacc atcgccagcg cgaggactgg atgcggcttc attggcacct cccttggccg
     1141 tagtagcagg tcgggatgcg gctggcgccg ttgccttgca gcgtggccac gtcgggcagc
     1201 atcggcacca gcgacgcgta gaactccgac aggtccatcg ccgcaaagtt gagcgtctga
     1261 agctgcgcga cggtgaagcc ggagcagtcg gcgctctgcg caccacccca gcccttgccg
     1321 atctgcgcgc ggccctgctc gttgacgatg cgcgcgagca tcgagttgaa gcagcacttc
     1381 gaggtcgtgt gttcgatgca ggacacgcag gcgccgagga tccgaatgca ggacgagcac
     1441 caggtgccga tcgtgtggca cagcttggcg ccttccttca tcgccagctt tccctcgtcc
     1501 tcgttgcagg acatcatcga caggatgatg tagatgacca cggcgatgac cagcgaccag
     1561 ggatcgaagg cgacgaccag gctgtcgccg gcatagagca cggacgagcc ggccggcagt
     1621 gcggtgccgt tgaccgcgac ggtcacgccg tagctcgtga agctgccgct gaagccggcg
     1681 ccggacagca gggccgacat gccctggtac aggaactccc ggttctcgga gttcatcagc
     1741 acgtcgtaga ccagccggga gccgctgccg aacaggcttt ggttggtcat gcccgcgccc
     1801 gcagcgtcgg cgtagcagca gttcttcagc aggcggtccc ggcagcggtt ctcctcaccc
     1861 ttgaacacct gcatgcggtc ggagtcgaga tacacgcccg cctcccggcc cgcctccagc
     1921 atcgacatgc tgcgcgcgaa gtcggcgtcg ttcggtgcgc tgatgtcgaa gcagttgccg
     1981 ttcaggcaga agacgttgga cgggcagttg ctggccgtgg tgacgctctg cgccggcacc
     2041 gggcagctgt agccgtcttc aacgacctcg cagacgcccg tggtggcgtt ggtctgcttg
     2101 cacgccgagc tctgcggggt gcaaccggcg gccaccagcg gcgcgcactg gtcgactggc
     2161 gctccgctgc tgcaggtcat cgtgctcgtg tactcccagc agtcgcgata caccgagatg
     2221 ccgtcgatca ccttggtcgc cgggccgtcg gtgcagacgg cggccgtgct gacggtgcag
     2281 cggccaccgg ccgcgagcgc cgggcattgg tcgtcccagc ggtcggttgt ggtcacggcc
     2341 gtggtcgccc gcgtgtaggt caaccgcatc gtcgggatcg ggccgtaggc cggattgggt
     2401 gcccaaccga ccacggcgcg atcggcgtcg atgttccagt agtagcccgg caccgtgccc
     2461 gtgatgtcgg taccggcgta gggtgtccag ccgccggccg gcgcataaca cttggcgcca
     2521 tccagcacgg tcgtcgtgca gccgttgtcg ccctgacaca gggtgtcctg gatgttgtcg
     2581 ccgctctgtg tgccggcacg gcaggccgcg tagatcttca ggaagggctc ggcgctggtg
     2641 cagctgaagc ccgagtcgcc gttgccggtg cagatctcct gtacctcggc ggcgatcatc
     2701 gcggtcaggc tgcaggtgct gccggcacag gacttgtccg ccacccacac gccggtgcgg
     2761 atcggcagac cggtcatgcc ggagtagttc gtctccagca ccgcgaccat ctcggggaag
     2821 accaccggcg tcgtcatgtt cacgtcgaag aaggtcagcg ggttgctgtc ctgcgtgacc
     2881 cggaagtgct gcgccgtttc cggccgatcg ggaaggcatt ggacgtcgat gccggcgcgg
     2941 ccggacccgg cgtgggcgaa ccagtcgccc ggtgtgcagc tcgtggtgcg atcggtggcg
     3001 accgtcagcg accgggcgca gctgtagttg ccgacaccca ggtagcgcag gcaactgcgc
     3061 gtctgggtcg tggcgggctg gttggtcacc gtggtcgtgc agccgctgta gtacgcggcc
     3121 aggctgccga gcacgctgga cgggctgcga ccgatatcgc gggcggcggc cacggcagga
     3181 tcgtcaggag agaccgcagg tcggggcgtg ttcgctgaag cgagcgcgcc gcgctgcgcc
     3241 tggcaggtcg ggtcgttcgg cagcgtcgcg cacagcgcca ggcgtgcgtt gccctgggac
     3301 gcgaggttcg gctgtcggta gtaggaccgc tccggcggcg tggtcgtgta gccgggcacg
     3361 acgccagtgg cgctgggggc ggtgacgctg ccgcgcgtca caggattcgc ggcctggccg
     3421 gcggcgacac cttcctcgtg cggaccggcc acgctcgcgg tctgcgtcgt cacgaagcag
     3481 acgagcgtga accagacgac caccttgcgc agcctcatcg caccgccccc ttcatgcgct
     3541 gcaggtagga cgatgcgtcg cgagccatgc cgggtgccga acgctcgaag aacctcagca
     3601 cgtagtccag gctgacgtcg ccggccgcca gcacgaactg gtcgctggcg atgcacatgc
     3661 cggcggcgca gggcttcgat gcggcgccct cgcgcaccag cacgaacgaa ggcgtgcgga
     3721 cgatcgagaa gcggtcgaag gcctgcgggt cgatctgcac ggcgacctgg cggttgccga
     3781 tcaggcgctg catgcgctcg accgtgtcgc gaagcgagcc gttgacgagc ccgcgcagca
     3841 ccagcgtggc ccgtgcgcga gaggcttggt ccagcagccg cgtgatcgtg gcttcgggca
     3901 ttgcgaagct gacgaagacc agcaggctgg gcccggaggc cgcaccgaga accggcttgt
     3961 ccgcctgcgc atcgaatccc ttggccagcg cctccaggtc gacaggcgtc ctggtcgcag
     4021 gttggggcag cgcgtcgata cgtggcgtcg aaggtacggg aacgcgggcc aactcggcgt
     4081 cggtgggcat cgggtgctgc tggcgcgcgc gctcgatgtc ggcgtccgtc actttgggca
     4141 gggtctggcc gagagccgcg ctctgaagca ccagcgccgc agcggcacag agcactgcgc
     4201 gtgtggtccg gcggcgggcc aggaggcgat cagtagccca cacagcagtt ccgcttcctg
     4261 aacagcatga aggcgaagtc ctcgccccgg acggggtact ccttgccggc gccccagacg
     4321 atcgtgctgc ggccgaaggg ctggcagcag cggcccccgt ccttcgcggt gttggccacc
     4381 gggtaggtga gctgggtctt gtaggcggtc ttgtccatcg ccggcaggaa gtaggggccg
     4441 cacagccccg gcttgccatg ccagccccac gccagcagct cgcggtgcat cttggcggtc
     4501 aggcgctgcg cgatcagggc cgccgtgcgg acgccgccca tgtggtacgg cacgtggccg
     4561 tcgaaggggt agatgccgcc ctggcacccc gcgcaccaga acatctcggc aatgccgaac
     4621 cctgcggtgg cggccacgca gtcggcggcg cacgccgcta cggctatcgg attcgcgaag
     4681 agcaccgcct cggggttgag gatcagcgtc agctcgtcgt cgttccacag cgggtcgacc
     4741 tcggtcaggt aggccaggtc gatagagccc cgttccaggc aggggaagtc ggtcaccacc
     4801 tccagccagt agaggaccgg gttcacgtag aagtgcgcct ggtagaagct gccgccgtcg
     4861 ccgtccccct ccgcccgcgt gaaacgcgcg gccgccgggg ccgagatgcc cgggtcgagg
     4921 tcgatgccgc ccagcgacgc caggcagaac ggtttgcgca ccgcctccac gtggcgggcc
     4981 ggttcccaga agccgatgga caggccgatc gtcgggttga cgccgcaact gcagaccggg
     5041 ttcgacgggt tggcgatgtc ctcctgcccg tcgacgttgg cgatcgtcgc gctgccgatg
     5101 ctgatcggca ggatgcaact ccagcagatc tcggtgatgg gattcgggaa gcggccggtg
     5161 caggtcaggc tgtcggcggc gtgggagagt gcgggcagca ggcctgtcgt cagcgcgagc
     5221 agggtcgcga gcacgaagtg gcatcgtggg cggcagacgt tcatggcagc cccaactcgt
     5281 cgacgcgcag ccgttggcct tcggcatccg gcaggtcccg gccctggtct cgcaggaagg
     5341 ccaacggctg cgc
//
LOCUS       DAOORV010000219         5326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18562_length_5326_cov_6.559666, whole genome shotgun sequence.
ACCESSION   DAOORV010000219 DAOORV010000000
VERSION     DAOORV010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18562_length_5326_cov_6.559666"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1112
                     /locus_tag="PKV56_17515"
     CDS             297..1112
                     /locus_tag="PKV56_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1845 domain-containing protein"
                     /protein_id="HOX69654.1"
                     /translation="MNTTRLAVIDTGQVNERILNKDEYRADFRKVEGASLKRTTHLSS
                     PHAKRMFARCFYSFQASMYFISQLGRAKLDHEVVERIEQNIREALDAATDEINKAIDQ
                     AEALLKHHNIETLASYDTVPLQEQIGITSSFGRRYFDLMHKLDIVMPMMETLAIEEII
                     SERELETRRSLYKRVVLSISTKARNLWMGVRRRMNERDARLAAEAAGAKPQASRPANR
                     GANDSIARDAAPEDPVVEADAGAAGDQAAAPVPSETPGSEVVGQEAEAVAASD"
     gene            complement(1109..1336)
                     /locus_tag="PKV56_17520"
     CDS             complement(1109..1336)
                     /locus_tag="PKV56_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69655.1"
                     /translation="MPADLMAIAHVRRLLLELGLEEAPERLIPAALGYSLWVSLPIAE
                     LEGKTPKELVADAEGSAALRRWIVVRLGLDS"
     gene            complement(1373..2497)
                     /locus_tag="PKV56_17525"
     CDS             complement(1373..2497)
                     /locus_tag="PKV56_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69656.1"
                     /translation="MLGSNTLRRWFGFMIASMRPQPLDLFDDSDPAAQARGFQEAFEQ
                     WLAHQRTSVSRKITLASSVRAYTVLWQAFAEWCLSQAPVVSLDSITEADLGAFINSRL
                     EPSAAARAKPSRLKEAGKFTPRHAWRLLTLIDGVLDVRARADASCSPNRSAATLLQSR
                     EEWLYANANRRDTLPDYLAPTEARVLVNYLSAGLPRSGRRGADITWQELRNRSAVALH
                     LGSGLTPADVRVLRLTSPVMRTGDEKGVPSTVHVPAHGDSPEREAPIIKWAGRVLAYW
                     QQVRKEQGLAGDFLFPATRSGKQWGKVTHYDAVKSVLEASGIDKSLVEGGAYRLRHTF
                     ALRQLRRGKSPDVVAKWLGVVEMRVMDRYRRVDYSSERPD"
     gene            2985..4871
                     /locus_tag="PKV56_17530"
     CDS             2985..4871
                     /locus_tag="PKV56_17530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOX69657.1"
                     /translation="MTEQGRAAEGPQVDVAGKAQGWDYSPDAGETARAMTANAVVSAV
                     ANVPLLLAAVALVVYMAFEAGLSTPGAVFGALGLTASGWRWSMSKRYRMREPFDEGTE
                     RSARREVELNAGLSGLMWGYGALAIYPHLREVAQIAYPMLLAGSAGVAALYAPLAGRA
                     YLYMIVPQVASLVAAHLAFDGLRSIGLAAIVAGFGFTMHRSAQLSKAAGQRLARALLE
                     RDRLRADHARLAELELTEAKLAAESARTEAAATRDLFIAKVSHEFRTPLQTIVALADL
                     LALKTADEKSKGLQEPVQRLSRAADQLMHQANDLAAFVRANQAASYEIRERPVRLDEV
                     VATSIIELRELANRKGVDVRVSVPAISLQADDMRLQQIIGNLVSNAVKYTTRGHIEIQ
                     AEVDDHEPHTMTLCVNDTGSGIPAEVLPNVFEPWFRGTREARGLGLGLSIVRSLVAQL
                     GGTAEIKSEIGRGTNACVRIPVKVLPQHPYHPPTGSSGLSDLHVLVVDDEDSVRNAVA
                     DLLRAKGAICVEAPGTEEALLELSTKPYEVALVDIQMPGADGYELARRLKARHPECET
                     RLIAMSAFGLSEEHGCLFDAYVAKPSKADTMVSTIIQTLARRGTVSARTAGVGGSGGR
                     SGAC"
BASE COUNT          976 a         1670 c         1831 g          849 t
ORIGIN      
        1 gacctttcgg cgcacgacga aacgggtcgc gcctcgaaag gctgatcggc ggatgtggat
       61 gtgcccgggg acgggcggcg cgtcggttcg acgctctaga gggcttgcct gatttgaggt
      121 gcagggccag ctactgcaca ggctagaacc ttagcgcctc gcctcggggg ttgtcaacga
      181 cgaagcgacg ggcgatttgg agggggggtt ggacccgcct ttacggtggc gcgcgccggc
      241 ggcgctcggg atgattgagg gacccgccca actccccttg gataacggag cacgccatga
      301 acacaactcg actcgccgtc atcgacaccg gccaggtgaa cgagcgcatt ctcaacaagg
      361 acgagtaccg ggcagacttt cgcaaggtcg aaggcgcttc gctgaagcgg accacgcacc
      421 tctccagccc gcatgccaag cgcatgttcg cgcgctgctt ctacagcttc caggcatcga
      481 tgtacttcat cagccagctc gggcgcgcga agctcgacca cgaggtggtg gagcgcatcg
      541 agcagaacat cagggaagcg ctggacgctg cgaccgacga gatcaacaag gcgattgacc
      601 aggccgaggc gcttctgaag caccacaaca tcgagacgct ggcttcctac gacacggtgc
      661 cgctgcagga acagatcggc atcacgtcct cgttcggcag gcgctacttc gacctcatgc
      721 acaagctcga catcgtcatg ccgatgatgg agacgctggc gatcgaagag atcatcagtg
      781 aacgcgagct cgagacgcgc cgatcgctct acaagcgggt cgtgctgagc atctcgacca
      841 aggcgcgcaa tctctggatg ggagtgcggc ggcgcatgaa cgaaagagat gcacgactgg
      901 ccgcggaggc ggccggggct aagccgcagg cctcacggcc ggccaaccgg ggggccaacg
      961 attccatcgc tcgcgatgcg gcgccagaag atcccgtcgt cgaagccgat gcgggggccg
     1021 caggggatca agcggccgcg cccgtgccgt cagagacgcc ggggtcggaa gtcgtcggtc
     1081 aggaggccga ggcggtggcc gcgagcgact agctgtcgag gccgagtcgc acgacgatcc
     1141 accgccggag cgccgcgctg ccttcggcgt cggcaaccag ttccttcggt gtctttcctt
     1201 ccagttcggc gatgggaagc gacacccaaa gcgagtagcc gagcgctgct gggatgagac
     1261 gctctggtgc ttcctcgagt ccaagttcca gcaggagcct gcggacatgg gcgatggcca
     1321 tcaggtcagc tggcatggct tgggaatcag gatccgaacc cagcaccggc cttcagtccg
     1381 ggcgttcgct cgagtagtcg acgcgtcggt agcggtccat cacccgcatc tcgaccacac
     1441 cgagccactt ggccaccacg tccggtgact tgccgcgacg aagctggcgc agcgcaaagg
     1501 tgtggcgcag gcggtaggcc ccaccctcga ccaggctctt gtcgatgccg gaggcttcga
     1561 ggaccgattt gacggcgtca tagtgggtca ccttgcccca ctgcttgcct gagcgcgtcg
     1621 cgggaaacag gaagtcgccg gcgagcccct gttccttgcg cacctgttgc cagtacgcca
     1681 gcacgcgacc ggcccacttg atgatcgggg cttcccgctc gggactgtcg ccgtgcgcag
     1741 gcacgtgcac tgtgcttggc acgcccttct cgtcaccggt gcgcatcacg ggagaggtca
     1801 ggcggagcac ccggacgtct gcaggggtca gccctgagcc gagatgcagc gcgacggcgg
     1861 accgattgcg cagttcttgc caggtgatat cggcgccgcg gcgccccgag cggggcaggc
     1921 cggcagacag gtagttgacg aggacgcggg cttcggtggg ggccaggtag tcgggcaacg
     1981 tgtcgcgccg gttggcattg gcgtagagcc attcctcacg ggattgcagc agcgtagcgg
     2041 ccgagcggtt tggggagcac gacgcatcgg cgcgtgcgcg aacgtcgagc acgccatcga
     2101 tgagggtcag caggcgccac gcatggcgag gcgtgaactt gcctgcctcc ttgagccggc
     2161 tgggcttcgc gcgcgccgcc gcactcggct cgagccggct gttgatgaag gcgcccaggt
     2221 ctgcctcggt gatgctgtcc aggctcacaa caggcgcctg gctcagacac cactcggcga
     2281 acgcctgcca gaggaccgtg taggcccgga cggaggaggc cagcgtgatc ttgcgcgaga
     2341 ccgaagttcg ttgatgtgcg agccactgct cgaacgcctc ttgaaagccc cgggcttgtg
     2401 ctgccgggtc ggaatcgtcg aacagatcga ggggctgggg gcgcatggag gcaatcataa
     2461 aaccgaacca acggcggagg gtgttgcttc ccagcatgcc tgcgctccag ggacgtgccg
     2521 acccacagaa gaagcctgcc aagaggtgag cgagcggcgg cgccgttggt tcggtttcga
     2581 agatggctgg gcggtcgcct tgtgtccctc gtagtgatgc accgcaaagt aggaccgaga
     2641 actcccccca tcggagggca gccatcgact cgctcgtttg ggcgatcggt ttgccagtgg
     2701 tcccgcgtaa acatgggggg tggacgcgct cgaatggagc ttgcccgcag acgatttgca
     2761 tgggctgttt cgatccgtcg ggtcgcagcg gagaaggaac gtggaaactc tgcgttcacc
     2821 gaagcgcgat tggatgcatg tcgaggcgac gcgcgtaacc catgtggcat ggcttgacgg
     2881 agcgcagacc gcaagcactc tggtgcatgg acaacaggaa aacaccaacc gggcggcacg
     2941 ccgctggagc aggcagacgg atcaatctca ggacgatacg aaccatgacc gagcagggca
     3001 gggccgcgga gggaccgcag gtcgacgtcg cgggcaaagc gcaaggctgg gactactcgc
     3061 ccgacgccgg tgaaacagct cgcgcgatga cggccaatgc ggtggtttcg gcggtggcca
     3121 acgtccccct gctcttggcc gccgtggctc tggtcgtcta catggccttc gaggcggggt
     3181 tgagcacacc gggtgcagtc ttcggggctc tcggcttgac ggcatccggt tggcggtggt
     3241 cgatgagcaa gcgctaccgc atgcgtgaac ctttcgacga ggggacggag cgatcggcac
     3301 ggcgtgaggt cgagctcaac gccggcttgt caggcctcat gtggggctac ggtgcactgg
     3361 cgatctaccc ccacctccgg gaagtcgcgc agatcgccta cccgatgctg ctggccggat
     3421 cggccggcgt ggcggccctg tatgcacccc tggcggggcg cgcttacctc tacatgatcg
     3481 ttccgcaggt ggcatcgctg gtcgccgcgc acctggcctt tgacggcctt cgctcgatcg
     3541 ggcttgcggc catcgttgcg ggcttcgggt tcacgatgca tcggtccgcg cagttgtcca
     3601 aggcagctgg ccagcggctc gcccgtgccc tcctggaacg agaccgcctg cgggcagacc
     3661 acgcgcgcct ggcagagctg gaactcaccg aagccaagct ggcggctgag tccgcgcgca
     3721 ccgaggccgc agccacccgc gacctgttca ttgccaaggt ctcacacgag ttccgaacac
     3781 cccttcagac catcgttgcc ctggcagacc tgctggccct caagacggca gacgagaagt
     3841 ccaaggggct ccaggagccc gtccaacgcc tgtcgcgggc tgcggaccag ctgatgcacc
     3901 aagccaacga tctggccgct ttcgttcgcg ccaaccaggc ggcgagctac gagattcggg
     3961 agcgcccagt gcgcctggac gaagtggtgg ctacttcgat catcgagctt cgggaactgg
     4021 ccaaccgcaa gggggtcgac gtgcgcgtca gcgtccctgc catctcgctc caggccgacg
     4081 acatgcgcct gcagcagatc atcggcaact tggtgagcaa cgctgtgaag tacacgacca
     4141 ggggccacat cgagatccag gcggaagtgg atgaccacga gccgcacacg atgaccctgt
     4201 gcgtgaacga caccggcagc ggaatccctg cagaggttct tccaaacgtc ttcgaaccgt
     4261 ggttccgtgg gacgcgcgaa gccagaggcc tgggactcgg gctctcgatc gtgaggtcac
     4321 tggtggcgca actcggcggg acggcggaaa tcaagtcgga gatcgggcgc ggaacgaacg
     4381 cctgtgtgcg cattcccgtg aaggtcctgc cccagcaccc ataccatcca ccaaccggtt
     4441 cctcaggcct gtcggatctt cacgtcctgg tggtcgacga cgaagactcg gtccgcaatg
     4501 cagtggcaga cctgctgcgg gccaaggggg cgatctgcgt ggaagcgcca ggcaccgagg
     4561 aagcgctgct ggagctgagt acgaaaccgt acgaggtcgc gctggtggac atccagatgc
     4621 ccggtgcgga tggctacgaa ctcgcccggc ggctgaaggc tcgccatccc gagtgcgaga
     4681 ccaggctgat tgcgatgagc gctttcgggc tgtcggagga gcacggctgc ctgttcgacg
     4741 cctatgtggc caagccgtcg aaggccgaca cgatggtcag cacgatcatc cagacgctgg
     4801 cacgacgggg aacggtctca gccagaacgg ccggggtggg tggctcgggt gggaggtcag
     4861 gggcctgctg aaagggacct ggagctacgg tccatcggag gcaccggacc ggatgtcagc
     4921 cgccccggaa cgacggaagg atgtcggtga cggacacggc ccgcaggtcg cctaccaacc
     4981 ttccgtgcac gtcggtgatg acaaggcctc ttcgcgctgc ctcccgacag agcgtttcca
     5041 gccgcggatc tgcaaagctc gttggcggat cgtacgcatg cacctggagg cctgcgaccg
     5101 ccagcaggcc gtcagcagac tgctggtcgc ggtgagcggc cgggccgcaa aggccagcga
     5161 gcgccgttcg caatggggag gtccgggact tcatggcggt acctgtgcag ggcacatcat
     5221 cgtgcgtgcc accgcagagc cgtgcaactc gaaaggccgg atctaccccc cgccgaaatc
     5281 ggccgtcgag tcccgttcaa gggctactcc gcggcgcgac ccagaa
//
LOCUS       DAOORV010000220         5326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18563_length_5326_cov_6.202049, whole genome shotgun sequence.
ACCESSION   DAOORV010000220 DAOORV010000000
VERSION     DAOORV010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18563_length_5326_cov_6.202049"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..741
                     /locus_tag="PKV56_17535"
     CDS             91..741
                     /locus_tag="PKV56_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX69658.1"
                     /translation="MNPPTKDTIPGLLLRSIPRGLVLGVEDALNAGAARAYAAAKGMD
                     EGHLPHVVGQLRHFNMNEAFYRALAAAEATPTAIQGNGLVTGRTGVFTLARFNIPEGF
                     WINGRRSQTRRQMAFANKAIEPLVQPELFDQYQPPAEAVAFFVACFSGSLQIQPESPT
                     SIQIAVPDRYMRGWLFREPVEAFAKRYDAQELSQIDLAVPKLKKNIGKKDVDGTSS"
     gene            786..1946
                     /locus_tag="PKV56_17540"
     CDS             786..1946
                     /locus_tag="PKV56_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOX69659.1"
                     /translation="MLAARRLTQAQLASLVGVSPATVSKWRAGTQAPEREALDRLAGV
                     VNVTPEWFTRPPAEKVSLPLFRSNASAHVAARAMLEARMEWAQDIALALTEFVDFPAL
                     NLPSRNFTDPEQITHEEIELATAECRDLWRLGRSAVQDLALAIEGAGVVLIREETGIA
                     QIEGLSAWSAVLGRPLVLLSADKDNGYRSRFDLAHELGHLILHRHIARPTDRERHKEL
                     EQQAHRFAGAFLLPAETFASEVRMPVTLDDLLLLKRRWGVSVGAILMRLRALKLLDDE
                     GAQALFKRRSARWGAKSEPGDGDRAPERPRLLRRTIDLLVDENVMPLEAIPRHLGLAS
                     HDIEMLAGLPEGYFQGRGNVVQLARIRQSADAPASSASAPSTVLPFRDPRRS"
     gene            1984..2793
                     /locus_tag="PKV56_17545"
     CDS             1984..2793
                     /locus_tag="PKV56_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOX69660.1"
                     /translation="MARTIYDKPTRALLKDMLAEWKLKPDETFSAQRAVEWFAKNYPK
                     LKAGSIHAHLVQASTNDPSRRHHPATNATDDLLFKVGSREYRRYQPGVDPAPIHQPGK
                     TQIAGAGGAMVTVDEEDDDAGVAVDAALAGSTQFALERDLQRYLADNLQLIEPGLTLF
                     QDEDITGFEYPAGGGRRIDILAKDKSGNFVVLELKVEKGYDRVVGQLLRYVNWVRKEL
                     AEPGQRVRGIIVCRTMSEDLVLACASNKEVELYEYRLQVTVSKVPALELPA"
     gene            2848..3306
                     /locus_tag="PKV56_17550"
     CDS             2848..3306
                     /locus_tag="PKV56_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69661.1"
                     /translation="MTVPRPLRNPEVCVNLWAYADEAGYIARVAARSYVLDGDDADKL
                     AVLHQLAATDFLHAEWMGVSKQFVISNPDGERMEGVAHASMLNDGVTPGVLFGPLFNQ
                     LEAAVPKQARTANGGYEAFSLKLPEDPLTVTTVVMEMDDGRLIPMVSNKD"
     gene            3613..4128
                     /gene="radC"
                     /locus_tag="PKV56_17555"
     CDS             3613..4128
                     /gene="radC"
                     /locus_tag="PKV56_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX69662.1"
                     /translation="MSTSSISSVGAADTLLVRDVDGQYRPAHADEVLCHARRLLANRV
                     RRGATMSSPQAVKDHLRLELGVLEHEVFCVLFLDAQHRIIALKQMFRGTVSQTSVYPR
                     EVVKEALSVNAAAVILAHNHPSGSVEPSRADEFLTQTLKTALALVDVRVLDHLVVAGA
                     DVCSFAERGLV"
     gene            complement(4166..4675)
                     /locus_tag="PKV56_17560"
     CDS             complement(4166..4675)
                     /locus_tag="PKV56_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HOX69663.1"
                     /translation="MTDPDSTWLLFDFSDPAAVTEWHAIDDRVMGGISSSRLRHDPAG
                     HAIFEGNVSLERNGGFASIRSTPADRGKPGSQTCFVEARATAKRFKLNLLTDDAFDSI
                     NYQASFDPSATWQTLHIPLSTFRATFRGREVPGAPALDPARIRQVGLMIAERQAGAFA
                     LEVRRIGLA"
     gene            4744..5049
                     /locus_tag="PKV56_17565"
                     /pseudo
     CDS             4744..5049
                     /locus_tag="PKV56_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            5107..>5326
                     /locus_tag="PKV56_17570"
     CDS             5107..>5326
                     /locus_tag="PKV56_17570"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX69664.1"
                     /translation="MLLDYENVQPSDAELRALVPDLGQVWVFHGPHQREVEKRFASFG
                     TAATAVPISKTGKNALDFHLSFYMGYIAS"
BASE COUNT          951 a         1640 c         1764 g          971 t
ORIGIN      
        1 cctggtgcct ccacgctccc ctctgtttaa gcgaaacaat actggtatag aatccgccta
       61 agcgaaatcg ctgacgaggt gactaagagc atgaatccgc ccaccaaaga cacgatccca
      121 ggcctgctcc tgcgcagcat tccgcgcggg cttgtgctcg gcgtcgaaga cgcactcaat
      181 gctggggctg cgcgcgcgta cgccgccgca aaggggatgg atgaaggtca cctcccgcat
      241 gtcgtgggcc agctcaggca cttcaacatg aacgaagcct tctaccgcgc gctcgcggcg
      301 gccgaggcga cgcctacggc catccagggc aacggcttgg tgaccgggcg cactggcgtc
      361 ttcaccctcg ccaggttcaa tatccccgag gggttctgga tcaacggtcg gcgcagccag
      421 acccgccgtc agatggcgtt cgcaaacaag gccatcgagc ccctggtgca gccggagttg
      481 ttcgatcagt atcagccgcc ggctgaagcc gttgcgttct tcgtggcgtg cttctcgggg
      541 tcgttgcaga tccaacctga atcgcccacc tcgatccaga tcgcagtgcc tgaccggtac
      601 atgcgcggct ggctcttccg cgagccggtg gaggcgtttg ccaaacgcta tgacgcccaa
      661 gagctcagcc aaatcgatct ggccgtgccg aaactcaaga agaacatcgg caagaaggac
      721 gtggacggca cctcgtcatg agccgcggtg gcgttcaagg atttcagtgt gatcgcctca
      781 gccagatgct ggctgcgcgc cgcctgaccc aagcgcagct ggcttcgctg gtgggcgtgt
      841 cgcccgcgac ggtgagcaag tggcgcgcgg gaacccaagc tccagagcga gaagcactcg
      901 atcgattggc cggcgttgtc aatgtgacgc ctgagtggtt caccaggccg ccggcggaga
      961 aggtttcctt gcctcttttc cgtagcaacg cctccgcgca tgtggctgcc cgggccatgc
     1021 tggaagctcg gatggagtgg gctcaagaca ttgcgttggc cttgaccgag ttcgtcgact
     1081 tcccggcctt gaatctgccg agccgcaact tcaccgatcc tgagcagatc acccacgaag
     1141 aaatcgaact ggccacggcg gagtgccgcg atctctggcg ccttggacgg tcagctgtcc
     1201 aggacttggc gctggccatc gagggcgctg gggtcgtgct catccgtgaa gagactggca
     1261 tcgcacagat cgaaggattg tctgcatgga gcgcagttct cggcagaccg ctggtgcttc
     1321 tctctgccga caaggacaac ggctatcgaa gccgattcga ccttgcccac gagctcgggc
     1381 atctgatcct tcacaggcac atcgcgcgtc ccaccgatcg agagcgtcac aaggaacttg
     1441 aacagcaggc ccacaggttc gcgggcgcgt ttctcctgcc cgcggagacc ttcgcatccg
     1501 aagtgcgcat gccggtgacc ttggacgacc tgctgttgct gaagcgtcgc tggggcgtat
     1561 ccgttggcgc gatcttgatg cgactgcggg ctctgaaact cctggacgat gaaggagctc
     1621 aggcgctgtt caagcgtcgg tcggcgcgat ggggcgccaa gtcggaaccg ggggatggcg
     1681 atcgagcgcc tgagcgaccc cgcttgctgc gtcgcacgat cgacctgctc gtcgatgaga
     1741 acgtgatgcc gctggaagcc attcctcggc atctcggcct tgcgtcgcac gatatcgaga
     1801 tgctggctgg gttgcccgaa ggctactttc aggggcgagg caacgtcgtt caactggccc
     1861 gcatccgtca gtcggcggat gcgcctgcca gttcagcgtc tgcgccgagc accgtgctgc
     1921 catttcgtga cccccgaagg tcttgatcga atccaccgct tgcttggaaa gtgagaacgc
     1981 cccatggccc ggaccatcta cgacaagcca acgcgggcgc ttctcaagga catgctcgcc
     2041 gagtggaagc tcaagcccga cgaaacgttc agcgcccagc gtgcggttga gtggttcgca
     2101 aagaactacc cgaagctgaa ggccggcagc atccacgcgc accttgttca ggcttcgacg
     2161 aacgacccga gtcgtcgcca ccatccggcg acgaatgcca ccgatgatct gctgttcaag
     2221 gtcgggtcac gcgagtaccg gcgctaccag cctggcgtcg acccagcgcc gattcatcag
     2281 ccgggcaaga ctcagatcgc tggcgccggt ggggcgatgg tcaccgtcga tgaggaggac
     2341 gacgatgctg gggtagccgt tgacgccgcg ctcgccggct cgacccagtt cgccctggag
     2401 agggacctgc agcgctactt ggccgacaac ctccagctca tcgagcccgg gctgaccttg
     2461 ttccaggacg aagacatcac cgggttcgag taccccgcgg gcggaggccg ccgcatcgac
     2521 atcttggcaa aggacaagtc gggcaacttt gttgtgctcg aactgaaggt cgagaagggc
     2581 tacgaccgcg tggtcgggca actgctgcgc tacgtgaact gggtacggaa ggagcttgct
     2641 gagccgggcc agcgcgtgcg cggcatcatc gtttgcagga cgatgtctga agacttggtc
     2701 ctggcctgtg ccagcaacaa ggaagtggag ctctacgagt accgcctcca agtgacggtg
     2761 tcgaaggtcc ccgctcttga gctccctgcg taagcgagta agcctgtttc atgatgctcg
     2821 gtattcaccg ccttcgggag acttgtcatg accgtgccgc gcccgctccg caaccctgag
     2881 gtgtgcgtca atctgtgggc ctatgccgat gaggctggct acattgcccg cgtcgcagca
     2941 agaagctacg tcctggatgg cgacgacgcg gacaagctgg ctgtcttgca tcaactggcc
     3001 gcaaccgact tcctgcacgc cgagtggatg ggtgtctcca agcagttcgt gatcagcaat
     3061 cctgatggag aacgcatgga aggcgttgcc catgcctcga tgttgaacga cggagtgacg
     3121 cctggagtgt tgtttgggcc actcttcaac cagctcgaag ccgcggtgcc gaagcaggcg
     3181 cgcacagcga atggaggcta cgaggccttc tcgttgaagc tgcccgagga cccgctgaca
     3241 gtaacgaccg tggtgatgga gatggacgac gggcgcttga tcccgatggt ctccaacaaa
     3301 gactgaacca cccgcacgat cggatgtgaa agtggctctt gccggtaggg gaaccacatt
     3361 cgcgccgact cggtgtactc gcggctaacg ccgttagggc ggcagtgccg ccgcacatca
     3421 caagttggac gacggtgctt ggaggttggg cttgccgtgt cgacccacgc taccacaccg
     3481 ttctcgccgt caaggtctgc gcgctcgcgg ggcgagcgct tgcggcctgc ggccgtcttc
     3541 gaaccctgac ggctgcgctg cggcgtggtg ctccggtctc gggcaatccc gcccgacaca
     3601 ccggagtcca tcatgtccac gtcttcgatc tcatccgtgg gtgccgccga tacgctgctg
     3661 gtccgcgatg tcgacggtca gtaccggccg gcgcatgccg acgaggtgct gtgccatgca
     3721 cggcggctgc tggccaaccg cgtgcggcgc ggtgccacga tgtcatcgcc gcaggcagtg
     3781 aaggaccatc tgcgcctgga gctgggtgtg ctggagcatg aggtcttctg cgtgctgttc
     3841 ctggatgcgc agcatcgcat catcgcgctg aagcagatgt tccgcggcac cgtgtcgcag
     3901 acctctgtct acccgcgcga ggtggtcaag gaagcgctgt cggtcaatgc ggcggcggtg
     3961 atcctggcgc acaaccatcc gtcgggatcc gtagagccga gccgtgccga cgagttcctc
     4021 acgcagacgc tgaagacggc gcttgcactg gtcgacgtgc gggtgctcga tcacctggtg
     4081 gtggccggtg ccgacgtgtg ttcatttgcg gagcgcgggc ttgtgtagct cagcgcaggc
     4141 gccgcgttgc ggcgccactt gccggtcacg cgaggccgat gcgccggact tcgagcgcga
     4201 aggcgccggc ctggcgttcg gcgatcatga ggccgacctg gcggatgcgg gcagggtcga
     4261 gtgccggtgc gccggggacc tctcggccgc ggaaggtggc gcggaacgtg gacaggggaa
     4321 tgtgcagcgt ctgccaagtc gcgctcgggt caaagctggc ctggtagttg atgctgtcga
     4381 aggcatcgtc ggtcagcagg ttcagcttga agcgcttggc tgtggcgcgg gcctcgacga
     4441 agcaggtctg cgagccgggt ttgcctcggt cggcgggcgt tgaccggatc gaggcgaagc
     4501 ctccgttgcg ttcgagcgag acgttgccct cgaagatggc gtggccggcc gggtcgtgtc
     4561 tcagtcggct gcttgagatg ccgcccatca cgcggtcgtc gatcgcgtgc cactcggtga
     4621 ccgcggcagg atcactgaag tcgaacagca gccacgtcga gtccgggtca gtcatggtgc
     4681 gattctccgg cgcgggtgcc gccgccgtgg ctggcaagat gcgtccaaag aagggagtca
     4741 gggatgaacc ttggtgaact gcaggctgag ttgcggcact tcgcggcaga gcgcgactgg
     4801 cagccgtttc acacgcccaa gaacctgacc acggccctca tggtcgaggc ggccgaactg
     4861 gcagaaatct tccagtggca gacgcctgat gagtcgcggc ttgcgcacgc ggatgccgca
     4921 gccaagcaac gcatcggcga ggaggtcgcc gatgtactgc tctacctgct gcaggtggcg
     4981 gatcactgcc aaatcgatgt gtcgcaggcg gtgcaggaca agctgacgag aaacgcagtc
     5041 aagtacccgg cgaagcgcgc gatcccgcgg gtgtcaccga cacgcgcatc ggtgcccggt
     5101 acgcatgtgc tgctggacta cgagaatgtc cagccgagcg atgccgagct gcgggcgctg
     5161 gtcccggatc tgggccaggt ctgggtgttt catgggccgc accagcgcga ggtcgagaag
     5221 cgtttcgcgt cgttcgggac cgctgccacg gccgtgccga tcagcaagac gggcaagaac
     5281 gcgctggact tccatctctc cttctacatg ggctacatcg cgtcgc
//
LOCUS       DAOORV010000221         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18608_length_5315_cov_7.150570, whole genome shotgun sequence.
ACCESSION   DAOORV010000221 DAOORV010000000
VERSION     DAOORV010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18608_length_5315_cov_7.150570"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKV56_17575"
     CDS             <1..326
                     /locus_tag="PKV56_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOX69665.1"
                     /translation="LRKMHFLMRSIALVCFPGGFGTLDELFETMTLIQTGKCRKRPIL
                     LFGREFWSRLINFDVLIETGMISPGDVHLFRYVETAEEAWAVLESEYGFDLPPTQTGE
                     FAADI"
     gene            341..1261
                     /gene="rocF"
                     /locus_tag="PKV56_17580"
     CDS             341..1261
                     /gene="rocF"
                     /locus_tag="PKV56_17580"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOX69666.1"
                     /translation="MRTNTHLSLIGAPTDIGAASRGASMGPEALRVANILPVLQGHGL
                     EVLDRGNLVGPTNPWLPATGGYRHLAEVAAWNRTVHDAVLAELRLNRLPVLLGGDHCL
                     GIGSISAVARHCRETGQRLRVLWLDAHADFNTHALTPSGNLHGMPVACLCGHGPKELI
                     ELSGQVPAINPKWVRQIGIRSVDAGEKRFVHEQELDVFDMRFIDEMGMRHAMELALAT
                     LDANTHLHVSFDVDFLDPPIAPGVGTTVPGGPTYREAQLCMEMIADTGRLASLDVMEL
                     NPALDVRNQTAEVAVDLIESLFGKSTLMRR"
     gene            complement(1282..1791)
                     /locus_tag="PKV56_17585"
     CDS             complement(1282..1791)
                     /locus_tag="PKV56_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOX69667.1"
                     /translation="MNRATCCAALMWVACAWSQVAAKPSEPTLTGVVTRVSDGDTLWV
                     RPDADGQPRKPVKVRLLGIDAPERCQAGGAESTAALTSRVLRREVAVRVVGRDGYGRL
                     LGEVRLDGQDLGAWMVREGHAWSHRRFGVADVYASEEAQARSAQRGLFGDPAAIEPHL
                     FRRRHGPCD"
     gene            complement(1805..2614)
                     /locus_tag="PKV56_17590"
     CDS             complement(1805..2614)
                     /locus_tag="PKV56_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOX69668.1"
                     /translation="MDFTSRPTSRKPIVVALGTFDGHSVRLDALQAHAAFDDFSAWLR
                     APGDWVGAFDLPFGLPRELVETLGWPTEWLSLIHHYAALSREQIRSTFAAFCDARPVG
                     GKFAHRACDGPAGSSPSMKWVNPPVAYMLHAGVPLLLAAGVHLPGLHAGAGAADACRV
                     AVEGYPGLLAREVLGSRSYKSDDKARQTPDRLIARKDLVDALEQGRTRLALRLRLSHA
                     QRDALVADASGDRLDAVLCMLQAGWARAAPDFGRPAGSDPLEGWIVTADAP"
     gene            complement(2759..3562)
                     /gene="xdhC"
                     /locus_tag="PKV56_17595"
     CDS             complement(2759..3562)
                     /gene="xdhC"
                     /locus_tag="PKV56_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HOX69669.1"
                     /translation="MNFLRDTACGWLASDEPAVLVEVLEAHGSTPREAGARMLVSTAA
                     VAGTVGGGHLELKAMEHARQMLHDGPAELQVRYPLGPSLGQCCGGVVTLGFSRLSAET
                     LAVWPDETARFHLQLHGAGHVGRAVARLLAELPVTVDWIDERDSEFPQTLGGAGWPAH
                     IRRHPADDVEGEVSLAAPGDRYLVMTHRHDLDLRITEAILKRGDFGFLGLIGSQTKRQ
                     RFVHHLESRGIPAAVLARMTCPIGLPGIAGKEPAVLALAVVAQLMTGRP"
     gene            complement(3627..4031)
                     /locus_tag="PKV56_17600"
     CDS             complement(3627..4031)
                     /locus_tag="PKV56_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69670.1"
                     /translation="MPRRPRIPPFTILAAELAWAVPQVVAHRLARMAVAGAAPSPRDR
                     REFALMNAEKNDAFHESWAAMTAQTLHVQQTLVTSLLRSIGLPPPSGASPSAEALMPW
                     NDAALGVLRHGMVPVHRRAVANAKRLGRTKLR"
     gene            4229..4861
                     /locus_tag="PKV56_17605"
     CDS             4229..4861
                     /locus_tag="PKV56_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HOX69671.1"
                     /translation="MMTTKNLKKVALVLALASSAAVPVAVQAQAQEGPWMVRVRALHL
                     DSRNKDSTGLDLSINNRWIPELDISYFLTPNIAAELVLTYPQKQDLRAGGDHIGSLKH
                     TPPTLLLQYHFTDFGNFKPYVGAGVNWTRISDVHFSPGVKAALHPSLDRSSFGAAGQI
                     GFDYKIAPNVYFNVDVKKVGIDTAVKSSGTKVGELKIDPWLIGFGIGYRF"
     gene            complement(4874..>5315)
                     /locus_tag="PKV56_17610"
     CDS             complement(4874..>5315)
                     /locus_tag="PKV56_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOX69672.1"
                     /translation="LLRADLLHDVGTSLNPAIDIGQIKGGFIQGMGWLTTEELVWHPA
                     DGSAQAGRLMTHAPSTYKIPTANDCPAVLDVRLYDAPNPADTIHRSKAVGEPPLLLSF
                     SVFFAIRDAVSAAGGHRIEPPLNAPATPEAILRAIMAVQATGTA"
BASE COUNT          926 a         1827 c         1740 g          822 t
ORIGIN      
        1 cgctgcgcaa gatgcacttc ctgatgcgca gcatcgcgct ggtgtgcttc cccggagggt
       61 tcggcacgct cgacgaactg ttcgagacga tgacgctgat ccagaccggc aagtgccgca
      121 agcggccgat cctgctgttc ggacgcgagt tctggtcgcg cctgatcaac ttcgacgtgc
      181 tgatcgaaac cggcatgatc tcgcccggcg acgtgcacct gttccgctat gtcgagaccg
      241 cggaagaggc ctgggccgtg ctcgagtcgg aatacggttt cgacctgcct ccgacgcaga
      301 ccggcgagtt cgcggcagac atctgagcgc cacgctggct gtgcgaacga acacccacct
      361 cagcctgatc ggcgcaccga ccgacatcgg ggctgccagc cgcggcgcga gcatgggccc
      421 ggaagcgctg cgcgtggcca acatcctgcc ggtgctgcag gggcacgggc tcgaggtgct
      481 cgaccgcggc aacctggtcg gcccgaccaa cccctggctg ccagccaccg gcggctaccg
      541 gcacctggcc gaggtggccg cctggaaccg cacggtgcac gatgcggtgc ttgccgagct
      601 gaggctgaac cgcttgcccg tcctgctcgg cggtgaccat tgcctgggca tcggttcgat
      661 cagcgcggtg gcacgccact gccgcgaaac cggccagcgg ttgcgcgtcc tttggcttga
      721 cgcgcacgca gacttcaaca cccacgcgct gacccccagc ggcaacctcc acggcatgcc
      781 ggtggcctgc ctgtgcggac atggaccgaa ggaattgatc gagctcagcg gccaggtacc
      841 tgccatcaat cccaagtggg tgcgccagat cggcatccgc agcgtcgacg ccggcgagaa
      901 gcgtttcgtg catgaacagg agctcgacgt cttcgacatg cgcttcatcg acgagatggg
      961 catgcgccac gcgatggaac tcgccctggc cacgctcgac gccaacaccc acctgcacgt
     1021 gagcttcgat gtcgatttcc tagaccctcc gatcgccccg ggggtcggca ccacggtgcc
     1081 gggcgggccg acctatcgcg aagcgcaact gtgcatggaa atgatcgccg acacgggtcg
     1141 cctggccagc ctggacgtca tggaactcaa ccccgccctc gacgtgcgca accagaccgc
     1201 cgaggtggca gtggacctga tcgagagcct gttcggcaag agcaccttga tgcggcgctg
     1261 acgcaacgca tccatgccgc gtcaatcaca cgggccgtgc cgccgcctga aaagatgggg
     1321 ctcgatcgca gcgggatcgc cgaacaaacc gcgctgcgcc gacctggcct gcgcctcctc
     1381 ggacgcatag acgtcggcca cgccgaagcg ccgatggctc cacgcatgcc cctcgcggac
     1441 catccaggca ccgaggtctt gcccgtccag gcgcacctcg cccagcaacc ggccgtagcc
     1501 gtcccgcccg acgacgcgca ccgccacctc gcgacgcagc acgcgcgacg tgagtgccgc
     1561 cgtggattcc gcgccacccg cctggcaacg ctccggcgca tcgatgccca gaagtcgcac
     1621 cttgacgggc ttgcgtggct gtccgtcggc gtcgggccga acccacagcg tgtcgccatc
     1681 gctgacgcgc gtgacgacgc cggtcagcgt cggttcggac ggcttcgccg cgacctgtga
     1741 ccaggcgcac gccacccaca tcagagccgc acagcaggtc gcccggttca tcggtctccc
     1801 gatctcacgg cgcatcggcc gtgacgatcc agccctccag tggatcggat ccggcaggtc
     1861 ggccgaagtc cggtgcggca cgcgcccaac cggcctgcag catgcacagc accgcatcga
     1921 ggcggtcgcc gctggcatcg gccaccagcg catcgcgctg cgcatggctc aggcgcagtc
     1981 gcagggcaag gcgcgttcgg ccctgctcga gcgcgtcgac cagatccttg cgggcgatca
     2041 ggcgatcggg ggtctgacgc gccttgtcgt cgctcttgta tgagcggctg cccagcacct
     2101 cgcgggccag caggcccggg tagccctcga ccgcgacccg gcacgcgtct gctgcaccgg
     2161 cgcccgcgtg cagaccgggc aaatgcaccc cggccgcgag cagcagcggc acgcccgcat
     2221 gcagcatgta cgcgaccggc gggttgaccc acttcatcga cggcgacgaa ccggccggcc
     2281 cgtcgcaggc gcggtgcgcg aacttgccgc cgacgggacg cgcgtcacag aacgcggcga
     2341 aggtgctgcg gatctgttcg cgcgacagcg ccgcatagtg atggatcagc gacagccatt
     2401 cggtgggcca acccagcgtt tcgaccagtt ctcgcggcaa gccgaacgga agatcgaagg
     2461 caccgaccca gtcgcccgga gcgcgcagcc aggcgctgaa atcgtcgaag gccgcatgcg
     2521 cctgcaaggc atcgagacgc acgctgtgcc cgtcgaaggt ccctagcgca acgacgatcg
     2581 gcttgcgcga cgtcggtcgg ctggtgaagt cgatgccggc tacgcggccc gggccggcgc
     2641 gggcttgcga ttgcgcctgt tgatcgaagt cgcttgcagg cgggtgcagg ggttcggaag
     2701 ccacggcatc gactctaacg atcgcccttg caccgggacg acgaagggtc gttcggagct
     2761 agggtcgacc ggtcatcagt tgggccacca ccgccagtgc cagcacagcg ggctccttgc
     2821 cggcgatgcc cggcagtccg atcgggcagg tcattcgtgc cagcaccgcc gcaggaatac
     2881 cgcgcgactc gaggtgatgc acgaagcgct gccgcttggt ctgcgatccg atcagaccga
     2941 ggaatccgaa gtcgcctcgt ttcaggatcg cctcggtgat gcgcaggtcg agatcgtgcc
     3001 tgtgcgtcat caccagatag cggtcccccg gcgcggccag ggacacttcg ccctccacat
     3061 cgtctgccgg atgcctgcgt atgtgcgcag gccagccggc accccccaac gtctggggaa
     3121 actcgctgtc gcgctcgtcg atccagtcga ccgtgaccgg cagttcggcg agcagccgcg
     3181 cgaccgcgcg accgacgtgg ccagcaccgt ggagctgcag gtggaagcgc gccgtctcgt
     3241 ccggccagac ggccagcgtc tcggcgctca gtcgcgaaaa acccagcgtg accacccctc
     3301 cgcagcactg cccgagggac gggccgagcg ggtagcgcac ctgcagttcc gcgggcccat
     3361 cgtgcagcat ctggcgggca tgctccatcg ccttcagttc gaggtgcccg ccaccgaccg
     3421 tgccggccac cgcagcggtg ctcaccagca tccgcgctcc cgcttcgcgc ggcgtcgagc
     3481 cgtgggcctc cagcacctcg acgaggaccg ccggctcatc gctggcgagc cagccgcagg
     3541 cggtgtcacg caggaagttc atcggtccat cgatgcgaag ccggcgtgat gcacgtcatc
     3601 gcaggccatc catgcgggcc cgcgcttcac cgaagcttcg ttcgacccag gcgcttcgca
     3661 ttggcaactg cgcggcggtg cacgggcacc atgccgtgtc gcagcacgcc gagcgccgca
     3721 tcgttccagg gcatcagcgc ttcagcggaa ggcgaagcac ccgagggcgg gggcagcccg
     3781 atggatcgca gcaacgacgt gacgagcgtc tgctggacat gcagcgtctg cgcggtcatc
     3841 gcggcccagg actcgtggaa ggcgtcgttc ttctcggcgt tcatcagggc gaattccctg
     3901 cgatctcggg gggatggagc agcacccgcc acggccatgc gcgcaagcct gtgcgcgacc
     3961 acctgaggca cggcccacgc gagctccgca gcgaggatcg tgaagggggg aatccgggga
     4021 cggcgtggca tgggaactcc tggagaagtg acaacgaatg tagtgggagc gagacgatcg
     4081 tgcagatcga gaaacacccc agcgaatcgg tcgggatgtt gctcgaacac acaacgcgca
     4141 gaaaccttga cgcaccgcaa cattcggtcc atccaacggg tatgaaattc ctttcgagga
     4201 gtgtttcctc tgcacgcagg acataaaaat gatgacaacc aagaatctga aaaaggtcgc
     4261 tctggtactg gctctcgcaa gcagcgcggc tgtgccggtg gcggttcagg cgcaggccca
     4321 ggaaggtcca tggatggtcc gtgttcgcgc ccttcacctc gacagccgca acaaggactc
     4381 gaccgggctc gacctgtcga tcaacaaccg gtggatcccg gaactcgaca tctcgtactt
     4441 cctcacgccg aacatcgctg ccgaactggt gctgacgtat ccgcagaagc aggatttgcg
     4501 cgcgggcggg gatcacatcg gctccttgaa gcacacgccg ccgacgctgc tgctgcagta
     4561 tcacttcacc gatttcggca acttcaagcc gtatgttggt gcaggtgtca actggacgcg
     4621 catctcggac gtgcatttct cgcccggcgt gaaggcagcg ctgcatccga gcctcgatag
     4681 aagcagcttc ggcgctgctg gacagatcgg cttcgactac aagatcgcac cgaacgtcta
     4741 cttcaacgtc gacgtgaaga aggtcgggat cgacaccgcc gtcaagtcga gcggcaccaa
     4801 ggtgggtgaa ctcaagatcg acccgtggct gatcggcttc ggcatcggct atcgcttctg
     4861 atcgaggttc ccctcaggcg gttcccgtcg cctgaactgc catgatcgcc cgcaggatcg
     4921 cctccggcgt tgccggggcg ttcagcggcg gttcgatgcg gtggccgccc gcggccgaga
     4981 ccgcgtcccg gatcgcgaag aacaccgaga acgacagcag cagcggcggt tcaccgaccg
     5041 ccttgctgcg atggatcgtg tcggccgggt tcggcgcgtc gtagagcctc acgtcgagga
     5101 ccgccggaca gtcgttggcg gtcggtatct tgtaggtgct cggtgcgtgg gtcatcaggc
     5161 gaccggcctg cgccgacccg tctgccggat gccacaccag ttcttcggtg gtcagccagc
     5221 ccatgccctg gatgaagccg cccttgatct ggccgatgtc gatcgcggga ttcaacgagg
     5281 tgccgacgtc gtggagcagg tcggcgcgca gcagc
//
LOCUS       DAOORV010000222         5303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18669_length_5303_cov_6.863567, whole genome shotgun sequence.
ACCESSION   DAOORV010000222 DAOORV010000000
VERSION     DAOORV010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18669_length_5303_cov_6.863567"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKV56_17615"
     CDS             <1..728
                     /locus_tag="PKV56_17615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX69673.1"
                     /translation="HLPGLDLLRAVAIVWVMLFHSFLVGGLGADFAWLSRFGWAGVDI
                     FFVLSGFLIGSQLLQQLERSGTLSLGGFYARRAWRILPAFAVVLAMYVFFPALREVPG
                     LQPWWQFASFTMNLLIDYERNPAFSHAWSLCVEEHFYLVFPLLALGLSRRASVPRFLA
                     ICAAVLLLGIALRGAAWLHFAAIDPPRNWFIEEIYYPCGSHRRCRACAFRFSSRTRWR
                     YCLPARCCTTWSSARSCAGAIGD"
     gene            1174..1593
                     /locus_tag="PKV56_17620"
     CDS             1174..1593
                     /locus_tag="PKV56_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69674.1"
                     /translation="MKLNMDPKRVLVVDDNEAFIEMAKFVLTAAGNVVDAASDSGSAA
                     LRIPVFAPDVILMDIQMPVVDGIELTKRLKADPTTRHIPVIAFTAYASKGEGHDLKAA
                     GFDGYIGKPVNVMTLAAEVAFWLEGPESARRSSFVWP"
     gene            1788..4532
                     /locus_tag="PKV56_17625"
     CDS             1788..4532
                     /locus_tag="PKV56_17625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX69675.1"
                     /translation="MEKGLDPDSVDVVRRLVDHVPSMLAYWAADLRCRFANRAYETWF
                     GADPDRLIGTSLRDLLGPELFALNEPFVVAALRGEEQTFERLVPGPDGIDRHSLVNFR
                     PDVIDGQVVGFFVTMTEVTHLKQTEANLRSAISALEEEIRRRRTAEYQLNDIQQSLAV
                     TLATIGAGFIATDRRGRVTQMNEVSERLTGWVQSEAVGHRLWDVFAREGRAASTLSTN
                     PVDWAAQQGLTVNDVHHVVAVARDGTRTPIEVKAALTFGEDGQARGLALVMRDRTRTM
                     QAKIDASRLAAIVESSNDAIISKTLDGRITSWNSAAQALFGYVADEAIGQPVQMLIPM
                     DRQDEEMRILVNLAQGVRVPAFDTVRRAKDGRLIEVSLTISPIRDADGTIVGASKIVR
                     DMSQSRQAEEARLKVEQLEAENRQIQQANRFKSQFLANMSHELRTPLNAIIGFSELLL
                     LGRVVPESPKHQVFLGHIASSGHHLLQLINDILDLSKVEAGKFEFFPEPVDLPKLVNE
                     VRDILQAGIERKGIALKVESDPQLDTIVLDPARLKQVLYNYLSNAIKFTPTAGYITVR
                     ATADGDEHFRLEVEDTGIGIAADQLPRLFTEFEQIDAGRNKQHQGTGLGLALTRRLVE
                     AQCGSVGVRSEPGKGSAFYARLNRIHGTDATRAEAGARPEPEARAHRLLVIEDDPHLQ
                     ARLVKGLLGAGYGVETASSAAQAARLVLGARFDGLTLDLLLPDRSGLELLADIRNEGN
                     SLRTRVMGVTVPVRGNGAASFAVADILAKPLRSGDLALAMARFRGPHGATSRVMVVDD
                     DPMALDLMRAALADVGIEAVCLQDGRRALAEIDQHQPQAIILDLMMPEFDGFAVLDAL
                     HQLPTWWDTPVFIWTSMLLTDDEYASLASSARTIVSKGGGDLIAMLERLKRWQPSVRA
                     "
     gene            4546..4968
                     /locus_tag="PKV56_17630"
     CDS             4546..4968
                     /locus_tag="PKV56_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69676.1"
                     /translation="MDSDPRVLIIEDNAANAELATFLLEDAGFVVNAVTDASDALAQV
                     ASFQPDLILMDIRLSGGDGLALTRAIKAGPSTSDIVVVAFTAHAMKGDEARLRDAGCD
                     AYVAKPIDVASFARQVEAALGAGPPMDLDTLRGEVAER"
BASE COUNT          919 a         1664 c         1727 g          993 t
ORIGIN      
        1 agcacctgcc cggcctggac ctgctgcgag ccgtcgccat cgtctgggtg atgctgttcc
       61 actccttcct cgtcggcggc ctcggggccg acttcgcgtg gctttcgcgt ttcggatggg
      121 ccggcgtgga catcttcttc gtgctcagcg gcttcctgat cggctcgcag ctgctgcagc
      181 aactcgagcg cagcggcacg ctctcgctgg gcggcttcta cgcgcggcgg gcctggcgca
      241 tcctgccggc gtttgcggtg gtgctcgcga tgtatgtgtt cttcccggcg ctgcgcgagg
      301 tgccggggct gcagccttgg tggcagttcg ccagcttcac gatgaacctg ctgatcgact
      361 acgagcggaa tccggccttc tcgcatgcct ggtcgctgtg cgtcgaagag cacttctacc
      421 tggtctttcc gctgctggcg ctggggctct cgcggcgggc cagcgtgccg cgcttcctgg
      481 cgatctgcgc ggcggtgctg ctgctgggca tcgcgctgcg cggcgctgcc tggctgcact
      541 tcgcggcgat cgatccgccg cgcaactggt tcatcgagga gatctactac ccttgtgggt
      601 cgcaccgtcg ctgccgggct tgcgcgttcc gcttttcctc acgtacgcgc tggcggtact
      661 gcttgccggc gcgctgctgc actacgtggt cgagcgcccg ttcctgcgct ggcgcgatcg
      721 gcgactgagc gcgccggctg ccaccgcctc gccgtccgcg gcttagcgcg gccgtcgcgg
      781 gaagactcgc gagcgaaggg gcgcttccgg ccacttgctg gtgctgctga ccggcggcta
      841 tgcggcggcc cagatagctc ggaacgaacc tagccgtttg cacggtcaag gaaggtcggc
      901 agtgccagcg gccaggggac acacgctctt gactggtgac acgctgatga atcggaacac
      961 gtctcattgg ggtggtcgct gaagtcgcgc gcttgcccgt acaacgatca ccaattccgg
     1021 agtcctgacg gacaggactg cttcagattg tggggtacag cctgacgaac gagcgccaat
     1081 gtacgttacc gaacagacga cacgaatgtc gctgcgccag cctcgcgcat ctatgcgatt
     1141 gcagcagtcg tagacacacg tccagttcaa ctcttgaaac tcaatatgga tccaaagcgc
     1201 gtgcttgttg tcgatgacaa cgaagccttc atcgagatgg cgaaatttgt tctgaccgct
     1261 gccggcaacg ttgtcgatgc ggcgtcggat tccggctctg cggccttgag gattccggtc
     1321 ttcgcgcctg acgtgatctt gatggacatc cagatgccag tcgtcgacgg catcgaactc
     1381 accaagcggc tcaaggctga cccgacgact cggcatatcc ccgtgatcgc cttcacggct
     1441 tatgcgtcaa agggcgaggg gcacgacttg aaggccgcag gcttcgatgg ctacatcgga
     1501 aaaccggtga acgtcatgac gcttgccgcc gaggttgctt tctggcttga ggggcccgaa
     1561 agcgctcgtc gaagctcctt cgtttggccc tgaaatgcag atacagcagc atggtttcga
     1621 agctctgcga taggttcatc tgcaggctgt ttgcagccgg ctgctcgctg caacgatggg
     1681 actggaaagc ctgcccacca tggacttgag gccatgagtc tcgtgggttg aacacgttcg
     1741 gcccagcgcg gccacgcggc ggcgaaccgg gcattgctgg ggaagcgatg gaaaagggcc
     1801 ttgacccgga tagcgttgac gtcgtgcgcc gcctggtgga ccacgttcct tcgatgctcg
     1861 cgtattgggc cgccgaccta aggtgtcgct tcgccaatcg ggcttatgaa acatggttcg
     1921 gagccgaccc ggaccggctc atcggcacgt ccttgcgcga cttgctgggc cccgagttgt
     1981 tcgcgctgaa cgaaccgttc gtcgtggcag cacttcgcgg cgaagaacag accttcgaac
     2041 gtctcgtgcc gggtccggac ggtatcgacc gccacagcct ggtgaacttc cggcccgacg
     2101 tcatcgacgg gcaagtggtt ggcttcttcg tcaccatgac cgaggtgacc catctcaagc
     2161 agacagaagc gaatcttcgc agcgcgatca gcgctctcga agaagagatc cgccgccggc
     2221 ggacggccga gtatcagctc aacgacatcc agcaaagcct ggccgtcaca ttggccacca
     2281 tcggcgccgg gttcattgca accgaccgcc gtgggcgggt cacgcagatg aacgaggttt
     2341 ccgaacgcct gacgggctgg gtgcaatccg aagcagtggg tcaccgcctt tgggatgtgt
     2401 ttgcccgcga aggccgcgcg gcgtccacac tttcaaccaa cccggtcgac tgggctgccc
     2461 agcaagggct gacggtcaac gatgtccatc atgttgtcgc cgtcgcgcgt gacggcaccc
     2521 gaacgccaat cgaagtcaag gcagcactga cctttggcga ggacggccag gcgcgtggcc
     2581 tggccttggt gatgcgagat cggacgcgca cgatgcaggc gaagatcgat gcaagtcgtc
     2641 tggccgcgat cgtcgagtcg tccaatgacg ccatcatcag caagacgctg gatgggcgca
     2701 tcacaagctg gaacagtgcc gcgcaggccc tgttcggcta tgtcgccgac gaggccattg
     2761 gccagcctgt gcagatgctc atccccatgg atcgccagga tgaggagatg cgcatcctgg
     2821 tcaatctggc ccaaggcgtg cgcgtgccgg ccttcgacac ggtacgccgc gccaaggacg
     2881 ggcgcctgat tgaagtctcg ctcacgatct ctccgatccg ggatgccgat ggcacgatcg
     2941 tcggcgcctc caagatcgtt cgtgacatgt cgcagagcag gcaggccgaa gaggcccgcc
     3001 tgaaggtgga acagctggaa gccgagaacc gccagatcca gcaagccaac cggttcaaga
     3061 gccagttcct ggccaacatg tcgcacgagc tgcgaacgcc gctgaatgcc atcatcgggt
     3121 tctccgaact gctcctgttg ggccgagttg tgccggagtc acccaagcac caggtcttcc
     3181 tcgggcacat cgccagcagc gggcatcacc tgcttcagct catcaacgac atactggacc
     3241 tgtccaaagt cgaagccggc aagttcgagt tctttcccga gccggtggac ctgccgaagc
     3301 tcgtcaatga agttcgcgac atcctccaag caggcatcga gcgcaaaggc attgccctca
     3361 aggtcgaatc cgatccgcaa ctcgacacga tcgttctcga tcctgcgcgg ctcaagcagg
     3421 ttctgtacaa ctacctgtcc aacgccatca agttcacgcc gaccgccggg tacatcaccg
     3481 ttcgcgccac ggcggacggc gacgagcatt tccgactgga ggtggaggac accggaatcg
     3541 ggatcgccgc cgatcagctg ccgcgcctgt tcacggagtt cgagcagatc gatgccgggc
     3601 gcaacaagca gcaccaggga actggcttgg ggctggcctt gacacgacgg ctggtcgaag
     3661 cccaatgcgg ctcggttggc gtgcgcagcg aacctgggaa gggcagcgcc ttctacgcga
     3721 ggctgaaccg aatccacggc accgacgcga cccgcgccga ggctggggcc cgaccggagc
     3781 cggaggcgcg agcgcaccgg ctcctggtga tcgaggacga tccgcatctg caggcgcggc
     3841 tggtcaaggg actgttgggt gctggttatg gggtcgagac cgccagctca gccgcccagg
     3901 ctgcacgcct cgttctgggg gcccggtttg acgggctgac cctggacctg ctcctgccgg
     3961 accgcagcgg actggaattg ctggccgaca tccgcaacga aggaaacagc ctccgcacaa
     4021 gggtcatggg tgtgaccgtg cctgtacgcg gcaacggcgc cgcatcgttt gctgttgccg
     4081 acatcctggc caagccgctg cgcagcgggg acctcgccct ggcgatggcg agatttcgag
     4141 ggccccatgg cgcaacctcc cgtgtgatgg tggtcgacga cgacccgatg gcgctggacc
     4201 tgatgcgtgc cgcgctggcg gacgttggca tcgaggcggt ctgcctgcag gacggccgcc
     4261 gagcccttgc cgagatcgac cagcatcagc cgcaggcgat cattctggat ctgatgatgc
     4321 cggagttcga cgggttcgcc gtgctcgacg cgttacacca gttgccaacg tggtgggaca
     4381 caccggtgtt catctggacc agcatgctgc tgaccgacga tgagtacgcg agtctggcca
     4441 gttcggctcg aacaatcgtc agcaagggcg gtggtgacct gatcgccatg ctggagcgac
     4501 tcaagcgctg gcagcccagc gttcgcgcgt gagaggtgcg gtgccgtgga ttccgatccg
     4561 cgcgtcctga tcatcgagga caacgctgcc aacgccgagt tggccacctt cctcctggaa
     4621 gacgcggggt tcgtcgtcaa tgcggtgacc gacgcaagcg acgcgctggc acaggttgct
     4681 tcgtttcagc ccgatctcat tctgatggac attcggctgt cgggcggcga cgggttggca
     4741 ttgacgcgag cgatcaaggc cggaccgtcc acgagcgata tcgtggtcgt ggccttcacg
     4801 gcccacgcga tgaagggcga tgaagccaga ctgcgcgacg caggttgcga tgcctacgtc
     4861 gccaagccga tcgatgtagc gagctttgct cgtcaggtcg aggccgcact gggcgcgggt
     4921 ccaccgatgg atctggatac gcttcggggc gaagtggccg agcgctgatg tgccagccgc
     4981 gcaccgcgcg gtccgttctg caagtgcaag ctcacgcttg tattgctgcg cgtgtttgct
     5041 gccgcagccc ttggctgatc ggccgatatc gaggctcaag gagggatcgg ttgcggccgc
     5101 agttgcgaaa cgacgggctg cttttcggcg atcagtttga agctatcgat gtctctcatg
     5161 ggtcgatagc ggaagtcgcg gacccgctcg tgccctgatc tgcgatcgcg accccggtca
     5221 ggccgagccg gcgcgcgcca cgtcgtcctg gatcagcgtc tccaggcact gctcgcgcag
     5281 cccgtagaag cgcttgatct cgg
//
LOCUS       DAOORV010000223         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18723_length_5295_cov_11.959733, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000223 DAOORV010000000
VERSION     DAOORV010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18723_length_5295_cov_11.959733"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="nifB"
                     /locus_tag="PKV56_17635"
     CDS             <1..1122
                     /gene="nifB"
                     /locus_tag="PKV56_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOX69677.1"
                     /translation="QEQAPDIKLCLSTNGLALPGMVDEICKYNIDHVTITINMVDPEV
                     GAKIYPWIFWEHRRVTGIEAATILHKQQMLGLEMLTARGVLTKINSVLIPGINDEHLY
                     EVNREVKKRGAFLHNIMPLISEPEHGTVFGLTGQRGPSAQELKAVQDACMGGANLMRH
                     CRQCRADAVGLLGEDRSEEFTLDKIDEMEVVYDLDKRKSYQDKVEAERQAQHAAKVAA
                     LAATNVDDGVDPDMKVLIAVATEGHGKVNQHFGHATEFQIFEVSQGSALFVGHRRVDL
                     YCQGGYGEEEALPSVVSAINDCHAVLVSKIGACPRDELKAAGIEPVDEYAHEFIEKAA
                     LSWFAGYRERIASGLITHQVRGDAQIRQGAYSGTAEQAA"
     gene            1167..1361
                     /locus_tag="PKV56_17640"
     CDS             1167..1361
                     /locus_tag="PKV56_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX69678.1"
                     /translation="MALKIIASMCTGCSACEPECPNVAIREKGGTFIIDPAKCTECEG
                     QFDSPQCIAVCPVDNCIKSV"
     gene            1397..1801
                     /locus_tag="PKV56_17645"
     CDS             1397..1801
                     /locus_tag="PKV56_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOX69679.1"
                     /translation="MLQPNVTVTPAAEKFIRRMVRFSEHPEGGFRLTVSAGGCSGYNS
                     EFTVVPAAVAGDSELLVNGVKVYLPAESRLMLDGVTVDFADTPTKSGLTFMNPNAAAC
                     GCSTSGDAAAAPAQATVSVSSIGRMPTATAGH"
     gene            1843..2493
                     /locus_tag="PKV56_17650"
     CDS             1843..2493
                     /locus_tag="PKV56_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69680.1"
                     /translation="MPNAELDRCAAATDEGAALSAVSVDFDDAVLGHGLPVQAEEALR
                     AAGAARADPPRAMAALMRARALAPDHPAVLIAFYRYHFYGHQLRLARGVSCEAMVVGA
                     RSLGLPLLWREVPDLPLPGAAGDPATRFYLWVLKGYAYLSLRLGEPEEARDALAKLRV
                     LDPEDRVGAALLEAVRQRRERDAGAGDEDEGPALPVFGAAAWARVAETGRASGAPG"
     gene            2490..3293
                     /locus_tag="PKV56_17655"
     CDS             2490..3293
                     /locus_tag="PKV56_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HOX69681.1"
                     /translation="MSQVVSAEWADIAARHWQGGELDCAACPHASMRLEGGCEPGRIC
                     MQDAYARRIDRFFRTHPDLANEHLGHPYFEVRAIAVRYADVFRLPALMHDPDETVRLH
                     LALRLPQRQLMLMREDAHREVRIRVAQCLAVDQIASMLHDEDYHVRATVARRLPEALL
                     PQMVSDPDLMVRREVARRVQMPALLRMTSDREAEVRRIVAERLPPALLDTFVHDDDLL
                     VRWEVAGRALPAVLEKLIHDADAEAEIRERARARLDQWQGEDQPEHEHG"
     gene            3286..3621
                     /locus_tag="PKV56_17660"
     CDS             3286..3621
                     /locus_tag="PKV56_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOX69682.1"
                     /translation="MADINREDEAIEVASPPRFNMGERVISRTVIRNDGTYNGKDIGE
                     VLVNKGEIGFITSIGTFLQQFYIYAVEFVESGHRVGMRAKELCTLDHLPEEVLAQLGE
                     KAGQLQDLR"
     gene            3657..3941
                     /locus_tag="PKV56_17665"
     CDS             3657..3941
                     /locus_tag="PKV56_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOX69683.1"
                     /translation="MIEQRQPRFEWGQAVTAAVDLYNDGSHPEAPEDQLLVVAGGPGE
                     IVQVGHHADANVPVYMVDFGVAVIGCLEEEIAAERALPGSVALPSGAAAA"
     gene            3938..4324
                     /locus_tag="PKV56_17670"
     CDS             3938..4324
                     /locus_tag="PKV56_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69684.1"
                     /translation="MNALNATGGGGGPRGSAEPPVRRFTGDVLSLDQRRIEKALEART
                     RYKYVHPRVEREGLGWKIVSPNCSRNIDAQGGEIDIAWLVPHSGGWLLFSRDHQQDCW
                     RLQRQDNGLSALLVHLCADPDREFWQ"
     gene            4321..>5295
                     /locus_tag="PKV56_17675"
     CDS             4321..>5295
                     /locus_tag="PKV56_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOX69685.1"
                     /translation="MSTASSTSVSEPVYLDHNATTAPCAEAIEDMLDILRTVWANPSS
                     THGPGQLARSALADARKRIAEFLGCQGAELVFTSGATESNHMALLGALALRKGSGRQR
                     IVMSAVEHAGSLALAARLQSQGHQVDLIPVDTQGRIDLEAAQALIGDDVALVSVMGAN
                     NETGVLMPIAELAALAHAAGSLMHVDATQLPGKTASRFDESGADLWSVSAHKLHGPKG
                     VGALVVRKGLAWPALFSGRQERQRRGGTENLPGIVGFAAAAARAGLTLDSDLAHMRRL
                     QVRLEAGLSAGLPEVHVYGASAQRLPNTSCLRFGTLDAEQVLGKLERAG"
BASE COUNT          892 a         1800 c         1803 g          800 t
ORIGIN      
        1 caggagcagg cacccgacat caagctgtgc ctgtcgacca acggcctggc cctgcccggc
       61 atggtcgacg agatctgcaa gtacaacatc gaccacgtca ccatcaccat caacatggtg
      121 gacccggagg tcggcgcgaa gatctacccg tggatcttct gggagcaccg gcgcgtcacc
      181 ggcatcgagg ccgcgaccat cctgcacaag cagcagatgc tgggcctgga gatgctcacc
      241 gcgcgcggcg tgctcaccaa gatcaactcg gtgctgatcc ccggcatcaa cgacgagcac
      301 ctctacgagg tgaaccgcga ggtcaagaag cgcggcgcct tcctgcacaa catcatgccg
      361 ctgatcagcg agcccgagca cggcaccgtg ttcggcctga ccggccagcg cggtccgtcg
      421 gcgcaggaac tcaaggcggt gcaggacgcc tgcatgggcg gcgccaacct gatgcgtcat
      481 tgccgccagt gccgcgccga tgcggtgggc ctgctcggcg aggaccgcag cgaggaattc
      541 acgctcgaca agatcgacga gatggaagtc gtctacgacc tcgacaagcg caagagctac
      601 caggacaagg tcgaggctga gcgccaggcc cagcatgcgg ccaaggtggc cgcgctggcc
      661 gcgacgaatg tcgacgacgg cgtcgatccc gacatgaagg tgctgatcgc ggtggccacc
      721 gaaggccacg gcaaggtcaa ccagcacttc ggtcacgcca cggagttcca gatcttcgag
      781 gtcagccagg gttcggccct gttcgtcggc caccgccgcg tcgatctcta ttgccagggc
      841 ggctacggcg aggaagaagc gctgccctcg gtggtcagtg cgatcaacga ctgccacgcc
      901 gtgctggtct cgaagatcgg cgcttgcccg cgcgacgaac tcaaggccgc cggcatcgag
      961 ccggtggacg agtacgcgca tgaattcatc gagaaggccg ccttgtcctg gttcgccggc
     1021 taccgcgagc gcatcgccag cgggctcatc acgcatcagg tgcgcggcga cgcgcagatc
     1081 cgccagggcg cctattcagg cacggccgaa caggccgcct gaagcccatt cattccgcaa
     1141 cccgcccacg tcccaaggag acccccatgg ccctcaagat cattgcctcg atgtgcaccg
     1201 gctgctcggc ctgcgagccc gagtgcccca acgtcgccat ccgcgaaaag ggcggcactt
     1261 tcatcatcga cccggccaag tgcaccgaat gcgaaggcca gttcgattcg ccgcagtgca
     1321 tcgccgtctg cccggtcgac aactgcatca agtccgtctg attccgccca cgacaacaca
     1381 ccacgagaca cacaccatgc tccagcccaa cgtcaccgtc acgcccgccg ccgagaaatt
     1441 catccgtcgc atggtgcgct tctcggagca cccggaaggc ggcttccgcc tgaccgtttc
     1501 ggccggcggc tgctccggct acaacagcga gttcacggtc gtcccggcag cggtcgccgg
     1561 tgacagcgag ttgctggtca atggtgtgaa ggtctatctg ccagcagaaa gccgcctgat
     1621 gctcgacggt gtcaccgtgg acttcgccga cacgcccacc aagagcggcc tgaccttcat
     1681 gaacccgaac gcggccgcct gcggctgctc gacctcgggt gacgcggccg cagcgccagc
     1741 ccaggcgacg gtatcggtca gctcgatcgg gcgcatgccc acggccaccg ctggccactg
     1801 atccctcgtt gcaggcgacg catcgtggat ccgatcctgt ccatgccgaa cgctgagctg
     1861 gatcggtgcg ccgcggcgac cgatgagggc gctgccctct ctgccgtcag cgtcgatttc
     1921 gatgatgcgg tgctcggcca cgggctgccg gtccaggctg aggaggcgct gcgcgcagcc
     1981 ggtgcggcgc gggccgatcc gccccgtgcg atggcggcgc tgatgcgcgc gcgtgcactg
     2041 gcacccgatc acccggcggt gctgatcgcg ttctaccgat atcacttcta cggccaccag
     2101 ttgcgcctgg cgcgtggcgt gtcgtgcgag gcgatggtgg tcggtgcccg gtcgctcggg
     2161 ctgccgctgc tctggcgcga ggtgccggat ctcccgctgc ccggggcggc gggcgatcct
     2221 gcgacgcgct tctacctgtg ggtgctcaag ggctacgcgt atctgagcct gcgcctcggc
     2281 gagcccgagg aagcgcgtga cgcgctggcc aagctgcgcg tgctcgaccc cgaggatcgc
     2341 gtcggggcgg cactgctgga agccgttcgg cagcgtcgcg agcgcgatgc cggcgcaggc
     2401 gacgaggatg aagggccggc gctgccggtg ttcggtgccg ctgcctgggc gcgtgtcgcc
     2461 gagacgggcc gtgcttcggg agcgccggga tgagccaggt cgtgtccgcc gaatgggccg
     2521 acatcgctgc gcggcactgg caggggggcg agctcgattg cgcggcctgc cctcacgcgt
     2581 cgatgaggct cgagggcggt tgcgagcccg gccgcatctg catgcaggac gcctacgccc
     2641 gacgcatcga ccggttcttc aggacgcacc ccgacctggc caacgagcac ctggggcatc
     2701 cgtacttcga ggtgcgtgcg atcgcggtgc ggtacgcgga tgtgttccgc ctgccggccc
     2761 tgatgcatga tccggacgaa accgtgcggc tgcacctggc gcttcgattg ccgcagcgcc
     2821 agttgatgct catgcgcgag gacgcgcacc gcgaggtgcg catccgcgtg gcgcaatgcc
     2881 tggcggtcga ccagatcgcc tcgatgctgc acgacgagga ctatcacgtg cgcgccaccg
     2941 tggcacgacg cctgcccgag gccctgctgc cgcagatggt ctcggatccc gacctcatgg
     3001 tgcgccgcga agtggcgcgg cgggtgcaga tgccggcgct gttgcgcatg acgtccgatc
     3061 gcgaggctga agtgcgccgc atcgtcgccg agcgtctgcc cccggcgctg cttgacacct
     3121 ttgtccatga cgacgatctg ctggtgcgct gggaagtcgc gggccgtgcg ttgccggccg
     3181 tgctcgagaa gctgatccac gacgccgacg ccgaggccga gatccgcgaa cgcgcccggg
     3241 cacgcctgga ccagtggcaa ggcgaagacc aaccggagca cgaacatggc tgacatcaac
     3301 cgggaagatg aagccatcga agtggcctcg ccaccgcgct tcaacatggg cgagcgggtc
     3361 atcagccgca ccgtgatccg caacgacggc acctacaacg gcaaggacat cggcgaggtg
     3421 ctggtcaaca agggcgagat cgggttcatc acctcgatcg gcaccttcct gcagcagttc
     3481 tacatctacg cagtcgagtt cgtcgaatcc gggcaccggg tcggcatgcg cgccaaggag
     3541 ctgtgcacgc tcgaccacct gcccgaagag gtgctggccc agctcggcga gaaggctggc
     3601 cagttgcagg acctgcgcta gggcagctta ccgaacgcat cacgagagcc aacgacatga
     3661 tcgaacaacg ccagcccagg ttcgaatggg gtcaagccgt gaccgcagcg gttgacctgt
     3721 acaacgacgg cagccatccg gaggctccgg aagatcagct gctggtggtc gccggcggac
     3781 cgggagagat cgtccaggtc ggacaccatg ccgatgcgaa cgtccctgtc tacatggtcg
     3841 acttcggcgt cgcggtgatc ggctgcctcg aggaggagat cgccgccgag cgcgcgttgc
     3901 cgggcagtgt cgcgctgccg agcggagcag ccgctgcatg aatgcactga acgccaccgg
     3961 tggaggaggc ggtccgcgcg ggtccgccga gccgcccgtg cggcgcttca ccggtgatgt
     4021 cctgtcgctg gaccagcgcc gcatcgagaa ggcgctggag gcgcgcaccc gatacaagta
     4081 cgtgcacccg cgcgtcgagc gggaagggct gggctggaag atcgtgagtc cgaactgctc
     4141 gcgcaacatc gatgcgcagg gcggcgagat cgacatcgcc tggctggttc cgcacagcgg
     4201 aggctggctg ctcttctcgc gcgaccacca gcaggactgc tggcgcctgc agcgtcagga
     4261 caacggcctg tccgccctgc tagtgcacct gtgtgccgat ccggaccggg agttctggca
     4321 gtgagcaccg catccagcac gtcggtgagc gagccggtct acctggacca caacgccacg
     4381 accgctccgt gcgccgaggc gatcgaggac atgctcgaca tcctgcgcac ggtctgggcg
     4441 aatccgtctt ccacccatgg cccggggcag ctcgcccgca gcgcgctcgc cgatgcccgc
     4501 aagcggatcg cggagttcct ggggtgccag ggggccgaac tggtgttcac cagcggcgcg
     4561 acagagtcga accacatggc cttgctcggc gcgctggctc tccgcaaggg cagcggtcgt
     4621 cagcgcatcg tcatgagcgc ggtcgagcat gcgggttcgc tcgccctggc cgcccgcctg
     4681 cagtcgcagg gccatcaggt cgatctgatt cctgtggaca cgcaggggcg catcgatctc
     4741 gaagcagcgc aggcgctgat cggcgatgac gtcgcgctcg tcagcgtgat gggggccaac
     4801 aacgagaccg gtgtcctgat gccgatcgcc gagctggcgg ccctggccca tgccgcaggc
     4861 agcctcatgc acgtcgatgc aacccagctg ccgggcaaga ccgcatcccg tttcgatgaa
     4921 agcggagccg acctgtggtc ggtgtcggcg cacaagctgc acggaccgaa gggtgtcggc
     4981 gccctggtcg ttcgcaaggg actggcgtgg ccggcgctgt tcagcgggcg ccaggagcgg
     5041 cagcggcgcg gcggcaccga gaacctgccc ggcatcgtcg gcttcgcggc cgccgccgca
     5101 cgcgctggcc tgacgctcga cagcgacctg gcccacatgc gacgccttca ggtccggctc
     5161 gaggccggcc tgtccgcggg actgcccgag gtgcatgtgt acggagcgag cgcgcagcgt
     5221 ctgcccaaca ccagttgcct gcgcttcggc acgctcgacg ccgagcaggt gctgggcaag
     5281 ctcgaacggg ccggt
//
LOCUS       DAOORV010000224         5264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_18887_length_5264_cov_7.654060, whole genome shotgun sequence.
ACCESSION   DAOORV010000224 DAOORV010000000
VERSION     DAOORV010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18887_length_5264_cov_7.654060"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKV56_17680"
     CDS             <1..224
                     /locus_tag="PKV56_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69686.1"
                     /translation="LLNSLRMEPGVFSECYITSPIGEGVARIILDPFSHLLFSNKLDD
                     NAPLDALRRQGLSIDDAITELLRRRGYTV"
     gene            299..580
                     /locus_tag="PKV56_17685"
     CDS             299..580
                     /locus_tag="PKV56_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69687.1"
                     /translation="MKPAQVIGIVDIAEVYRAKESEFAALLTASKTEDDRQRAYAQAQ
                     AFGDRLDRALREIPNECRCLVVVKSAVAGGWSNAIDLTAALKAKVGAKS"
     gene            622..1155
                     /gene="traF"
                     /locus_tag="PKV56_17690"
     CDS             622..1155
                     /gene="traF"
                     /locus_tag="PKV56_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HOX69688.1"
                     /translation="MLHGSRVVLFDLRRHWVMSVLVAAIWVLALVRLFAHHVPVLPLL
                     FNWTPSLPYKVALVDFRSSSIDRGDLVVYAFEGPAGEKAYPGLRHQALFKRVAGVAGD
                     VVTVQGRDVFVNGVLVGRAKTHAFDHRLLDPIAPTVIPAGHLYVQGTSPDSFDSRYSL
                     SGLVRVQDVRSRVIPLL"
     gene            1163..1492
                     /locus_tag="PKV56_17695"
     CDS             1163..1492
                     /locus_tag="PKV56_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69689.1"
                     /translation="MHRIFLLSILCLAGTVAAQGERLDLQDDVPIDTYLALLAQVAPP
                     ARDGAEAYMAAFRSRCGRALRTVELRRAFAQGSGDPVLMNMVRASHERDTAALQRLGA
                     SIACPAK"
     gene            1494..2123
                     /gene="traW"
                     /locus_tag="PKV56_17700"
     CDS             1494..2123
                     /gene="traW"
                     /locus_tag="PKV56_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HOX69690.1"
                     /translation="MRKSQLPALLLALACWAGAAHSDNLGTIGPTYPVAERNLLEVIM
                     ARLRAKEASGELKRHEREARDRATYAVNNPRPVDGLRRAEAARTFYFDPSFALQSNVI
                     DSKGAVLFPAGTRKNPLEVVSLSKHLLFFDARDPGQVNKARELIDRYQGKVKPILVGG
                     SYLDLMKRWNKPVFYDQEGALVRKFGIAAVPAIVSQEGLRLRIDEVPVR"
     gene            2120..3133
                     /locus_tag="PKV56_17705"
     CDS             2120..3133
                     /locus_tag="PKV56_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HOX69691.1"
                     /translation="MNLFQRIFAVLCLLVSSSATAAGMATCTGKFPNPLTDICWSCLL
                     PITIGGATIANFGGQEDNGDNPANPICSCGVNPTIGLSIGFWEPARHVEATRKPFCLP
                     SLGGLNLDPGIAAPEAARFTRPEGDGDGGSFYQAHFYTNPVLYWLEVVTDFPCLERGS
                     FDLAYLTEVDPLWNDDELTLILNPDAVLFANPIAIAACAADCVAATVGFGLNTLFWCA
                     GCQGGIYPLDGHVQYHMGGVRTAALLAQRLTAKMHRELIAWGWHGSRGLCGPYFEPIM
                     DKSSYKTNLTYPIPSTDKLDGKCCQPFGRTTVLWGAGKEYPVLGEDFAFMLFRKRNCC
                     VGY"
     gene            3120..3887
                     /gene="trbC"
                     /locus_tag="PKV56_17710"
     CDS             3120..3887
                     /gene="trbC"
                     /locus_tag="PKV56_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HOX69692.1"
                     /translation="MLATELLQARRVLEAAALLLALQAASVFAQSPRSPVTDADVARA
                     ARNQPVITDKEIDAARLKHRMPTDEELARVPVPSAPRIDALPQPQTQGKIDLGAIAGG
                     FDAMGSPDPAKAGMPVGPTLLVFVSFSMPEPALERLVDQAARTGATLVLRGLVEDSMQ
                     KTVARVQRVIGQRKVGFQIDPQAFDRFAIAATPSFVLIKDRSVPTPCAAGACFSADSF
                     ALAAGDVSIDYALRFIQRTAPKYSREAQVFLAKMKGG"
     gene            3894..>5264
                     /gene="traN"
                     /locus_tag="PKV56_17715"
     CDS             3894..>5264
                     /gene="traN"
                     /locus_tag="PKV56_17715"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HOX69693.1"
                     /translation="MFKRLIVWVMTQALILAQTAAIAQTSHDQGVAAGRAANTTIRGM
                     VNQPSATGVVPGYTTTPPEAALAGRPALGPDVSAKLAACAATPNDPSCQALRTAITSA
                     NTPRPTISPNDPSVAAASRIARNPSLDLGSLASYYSGCTTTDATIATHTETRACARYV
                     TAGSYTCSNSLNVAVDRTTTCAPGDWFAQASSGSTAVATQCLPDRPDSSQHFRVTSSG
                     TPLAFFDVDMTTSVITPQRVAVIGTSYNWPSFTPSERGVYVADKSCSGTTCSLTAMIA
                     DDPVESCTGGWESMTCTTIHPFIDVYAACPAGTQSGNNLLVTTCSYGGDGMECTSSPL
                     STTTCYSPSGSPGDIQASDITGTFSSPYWRASSTRAVVGWAPNPAYGPIPQMRLSYTK
                     PATTYTAADTWNNQCPSLAADSRCSVASAPVCVDGPATKVIDGVSITRDCWQYQSTMS
                     CGGTYTA"
BASE COUNT          890 a         1856 c         1585 g          933 t
ORIGIN      
        1 ggctgctgaa ctcgttgcgc atggagcccg gcgtgttctc cgagtgctac atcacatcgc
       61 ccataggcga gggggtcgcg cgcatcatcc tggacccctt ctcccacctg ctcttcagca
      121 acaagctgga cgacaacgcg ccgctggatg ccctgcgcag gcaaggcctg tcgatcgacg
      181 acgccatcac cgagctcctc cgtcggcggg ggtacaccgt atgagctccc ttgccaccca
      241 agccctcgtg tccgtcttcg tgactgcagg ggccctgctg gcgtacgacc gccttgtcgt
      301 gaagcctgcg caggtaatcg ggattgtcga catcgccgag gtctaccgcg ccaaggagtc
      361 ggagttcgcc gcgctgctca cggccagcaa gaccgaagac gatcggcagc gagcctatgc
      421 gcaggcgcag gccttcggcg accgcctgga tcgcgcgctg cgcgagatcc caaacgagtg
      481 ccgctgcctg gtggtcgtca agtccgccgt tgccggtggc tggtccaacg caatcgacct
      541 caccgccgcc ctcaaggcga aggtcggcgc caagtcgtag ccaacgccat gtcaacttcg
      601 cgagtcggtc tgagccagtg gttgctgcac ggcagccgcg tggtgctatt cgacctgcgg
      661 cggcactggg ttatgtccgt tctcgttgcc gcaatctggg tcctcgccct cgtccgcctc
      721 ttcgcccacc acgtcccggt cctgcccctg ttgttcaact ggacgcccag cctcccctac
      781 aaagttgcac tcgtcgattt ccgatcgtcc tctatcgacc ggggcgacct ggtggtctac
      841 gccttcgagg gcccggctgg cgagaaggcg tatccaggtc tgcggcatca ggctctgttc
      901 aagcgggttg cgggcgttgc tggcgacgtg gtgaccgtcc agggccgcga cgtgtttgtc
      961 aacggcgtgc tggtgggccg cgcaaagacg catgccttcg accatcgctt gctcgatccg
     1021 atcgccccga ccgtcattcc cgccggtcac ctgtacgtgc agggaacaag cccggacagc
     1081 ttcgattccc gatacagcct gagcggactt gtgcgcgtgc aagacgtcag gtcgcgggtc
     1141 atccccttac tgtgaggacc gcatgcaccg gatcttcctg ctgtcgatct tgtgcctcgc
     1201 aggcacggtg gccgctcaag gcgaacgcct ggacctccag gacgacgttc cgatcgatac
     1261 ctatctcgcg ctgctggctc aggttgcgcc gccggcgcgc gacggcgccg aggcctacat
     1321 ggctgcgttt cgcagccgct gtggccgcgc cttgcgcacc gtcgaattgc gccgcgcatt
     1381 cgcccaaggc agcggtgatc cggtgctgat gaacatggtc cgagcgtccc acgagcgcga
     1441 taccgccgcc ttgcagcgtc tgggcgcctc catcgcctgc ccggccaagt gacatgcgca
     1501 agtcccaact ccctgcgctc ctcctggcac tggcgtgctg ggcgggcgcg gcgcattccg
     1561 acaacctcgg cacgatcggc ccgacctatc cggtcgcgga gaggaatctc cttgaggtga
     1621 tcatggccag gctgcgagcc aaggaggcaa gcggcgaact caagcgccac gagcgggagg
     1681 cacgagatcg cgcgacctat gccgtcaaca acccgcggcc ggtcgatggt ctccgacgag
     1741 ccgaggctgc acggacgttc tacttcgacc cgagcttcgc cctgcagtcc aacgtcattg
     1801 acagcaaggg agccgttctc tttccggcgg gaacgcgcaa gaaccccctc gaggtcgttt
     1861 ccctctccaa gcacctgctg ttcttcgacg cccgcgatcc gggccaggtg aacaaggccc
     1921 gtgaactgat tgatcgctac cagggcaagg tcaagccgat cctagtgggc ggcagctacc
     1981 tggacttgat gaagcgctgg aacaagcctg ttttctacga ccaggagggg gcactcgtcc
     2041 gcaagttcgg gatcgcggct gttccggcca tagtctcgca ggaggggctg cgcctgcgca
     2101 tcgatgaggt gcccgtgcga tgaacctctt ccagcgcatc ttcgcggtcc tgtgcttgct
     2161 ggtttcgtcg tctgcaacgg cagccggcat ggcgacctgc acggggaagt tccctaaccc
     2221 gctgaccgac atctgttggt cctgcctgct gccgatcacc atcggcggcg ccacgatcgc
     2281 gaacttcggc gggcaggagg acaacggcga caacccggcc aacccgatct gcagctgcgg
     2341 tgtcaatccg acgatcggcc tgtcgatcgg attctgggag ccagcgcgcc acgtcgaagc
     2401 gactcgcaag ccgttctgcc ttccctcgct gggcggtctc aatctggatc ctggcattgc
     2461 cgcgcctgag gccgccaggt tcacacgccc ggagggcgat ggcgacggcg gctccttcta
     2521 ccaagcgcac ttctacacca acccggttct ctactggctc gaggtcgtca ccgacttccc
     2581 gtgccttgag cgcgggagct ttgatctcgc ctacctcacc gaggtcgatc ccctctggaa
     2641 cgacgacgag ttgaccttga tcctgaaccc ggacgccgtg ctcttcgcca atccgattgc
     2701 tatcgccgcc tgtgcagcag actgcgtggc agcgacggtc ggcttcggcc tgaacaccct
     2761 cttctggtgt gccggctgcc aaggcggcat ctaccccctg gacggtcacg tgcagtacca
     2821 catgggcggc gtccggaccg cagccctgtt ggcgcaacgc ctcacggcga agatgcatcg
     2881 tgaactgatc gcctggggat ggcacggctc gcgcgggctc tgtggcccgt acttcgagcc
     2941 gatcatggac aagtcgtcct acaagacgaa cctcacctac cccatcccat cgaccgacaa
     3001 actcgacggg aagtgctgcc agcccttcgg ccgcacgacg gtcctctggg gcgccggaaa
     3061 ggagtacccg gtcctgggcg aggacttcgc cttcatgctt ttccgcaaga ggaactgctg
     3121 tgttggctac tgaacttctc caagcccgcc gcgtcttgga agccgcggcg ttgctgctgg
     3181 ccttgcaggc tgcctcggtt ttcgctcagt caccgaggag cccggtgacc gatgcagatg
     3241 tcgcacgcgc ggcgcgcaat cagccggtca tcaccgacaa ggagatcgac gcggcgcgac
     3301 tcaagcaccg catgcccacc gacgaagaac tcgcacgggt gcccgttccc tctgcgccgc
     3361 gcatcgacgc tctgccgcag ccgcagaccc agggaaagat cgaccttggc gccatcgccg
     3421 gcggcttcga tgcaatgggc tccccagacc ctgccaaggc tggcatgccc gtgggcccaa
     3481 cactcctggt gttcgtgagc ttctcgatgc ccgagccggc gctggagcgc ctggtcgacc
     3541 aagctgcacg cactggagca accctggtgc tgcgcggcct ggtcgaggac tcgatgcaga
     3601 agactgtcgc ccgtgtgcag cgcgtgatcg gccagcgcaa ggtcggcttc cagatcgacc
     3661 cacaggcttt cgaccgcttc gcgatcgccg ccacaccctc cttcgtgctg atcaaggacc
     3721 ggtcagtgcc aaccccctgc gcggcagggg cgtgtttctc ggcagacagc tttgccctgg
     3781 ccgccggcga cgtgagcatc gactacgcgc tgcgattcat ccagcggacc gcgccgaagt
     3841 actcgcgcga agcacaggtc tttctggcca agatgaaagg aggttgaacc gtgatgttca
     3901 agcgcctgat cgtctgggtc atgacccagg ctctgatcct cgctcagacc gctgcgatcg
     3961 cacagacctc gcatgaccag ggcgtcgccg cggggcgagc ggccaacacc accatcaggg
     4021 gcatggtgaa ccaaccctct gcgacaggtg tcgtgcccgg gtacaccacc acgcctcccg
     4081 aggccgcttt ggccggacgc ccagccctcg ggcccgatgt gagcgccaag ctggccgcat
     4141 gtgcggcgac gccgaacgac cccagttgcc aggcgctgcg gacggccatc acgtctgcga
     4201 acacccctcg ccccacgatc tcaccgaatg atccctcagt tgccgcagcg tcacgcatcg
     4261 cgcgcaaccc ctcactggat ctggggagcc ttgcctccta ctacagcggc tgcacgacca
     4321 ctgatgccac catcgcaacc cacacggaga ctcgtgcctg cgctcgctat gtgaccgcgg
     4381 gcagctacac ctgctcgaac tcgctcaatg tcgcggtcga tcgcacgacc acctgcgccc
     4441 ccggcgactg gttcgcgcag gcgtcctctg gctcaactgc agtcgccacg cagtgcctgc
     4501 cggatcgacc ggactccagc cagcactttc gggtcaccag ttccggcacg ccgctggcgt
     4561 tcttcgacgt cgacatgacg acgtcggtga tcaccccgca gcgggtcgcg gtgatcggca
     4621 catcctacaa ctggccgagc ttcaccccct ctgaacgagg tgtctacgtc gctgacaagt
     4681 cgtgttccgg cacgacctgc agtctcaccg ccatgatcgc cgacgacccc gtcgagtcgt
     4741 gtacgggcgg ctgggagtcg atgacctgca cgaccatcca tcctttcatc gacgtctacg
     4801 ccgcctgtcc cgctggaacc cagagcggca acaaccttct cgtcaccacc tgctcgtacg
     4861 gtggtgacgg gatggagtgc accagctcac cgctcagtac caccacttgc tacagcccca
     4921 gcggctcgcc gggggacatc caggcaagcg acatcaccgg caccttctcg tccccctact
     4981 ggcgcgccag ctcaacccga gccgtggtcg gctgggcacc gaatccggca tacggtccga
     5041 tcccgcagat gcggctctcg tacaccaagc ccgcgacgac gtacaccgcc gcagacacct
     5101 ggaacaacca atgccccagc ctcgccgctg acagccggtg ctcggtggca agcgcacctg
     5161 tgtgcgtgga tggccctgca acgaaggtga tcgacggcgt atctatcacc cgagactgct
     5221 ggcagtacca gagcacgatg agttgcggcg gcacctacac ggcc
//
LOCUS       DAOORV010000225         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19204_length_5197_cov_10.074874, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000225 DAOORV010000000
VERSION     DAOORV010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19204_length_5197_cov_10.074874"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKV56_17720"
     CDS             complement(<1..466)
                     /locus_tag="PKV56_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69694.1"
                     /translation="MPDPIPLHGTSLHDGVAARLRELVFQHELAPGLFIDELALAARW
                     QISRTPLREALKVLAAEGLVELVPRRGARVVALTDADAEQLFPVMAMLEGRCALEATC
                     RATVDDLAQLQALHDKLERAAAAADVDGYYRANHDFHSLVQAVAANRWLDRAT"
     gene            complement(614..1417)
                     /locus_tag="PKV56_17725"
     CDS             complement(614..1417)
                     /locus_tag="PKV56_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69695.1"
                     /translation="MNGSTPHTREASPVHWQDATDPRGHATREFRLTRAGGRSVTGAL
                     WLPAQPRPDQPLVLCGHGASGNRYQAPIASLAQRFTQEAGCALLSLDGPVHGLRQVGA
                     GGREAFFPEIQRPGSIDDMVADWAVAIDAARAVPGLRGARLAYFGLSMGSIYGIPLLA
                     ARNDVTVATLGLLGSQGAAAHLGTRLLADAARIQCPVVFLVQLEDELFGREGCLALFD
                     ALGSADKRLHANPGLHPAVPQEEIDFAFGFLRDRLEAQAPRRIVNLISD"
     gene            1957..3117
                     /locus_tag="PKV56_17730"
     CDS             1957..3117
                     /locus_tag="PKV56_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOX69696.1"
                     /translation="MKKVFAMFALTAAMPLAFGAVVNLNIIQVCDDAGLSCAAVNLNQ
                     SFTDKIWAQTGTTFNYGSITQLNSSKYLNPTEAEAGSLISSFFGTGREFFAYDVWFVN
                     GFVGAPGLRGLGALGGDGLVVSSSTAAVDTLAHELGHNFGLDHTDAAVLNADRYLMAS
                     GGIRTIPTTVANVAPDGLQLSRLNPILPNVTVDMIGATPFESSDFFDVKYLAGAASDL
                     GLRSLTINLAPANAFFDITDFEPGTAGSPFSYGRLDGVSASDIAISGLTDGSSLLTMR
                     FAEDSFTSGDSIAFGVDMDLFSNIDGFGATADELRLAEVTLTFEDGHSVAGDLSTLIF
                     ASEFDPNRNLDVLTTRLADPISAVPAPPSVLLFGFGLALLGALRWRPVRQFS"
     gene            complement(3688..4983)
                     /locus_tag="PKV56_17735"
                     /pseudo
     CDS             complement(3688..4983)
                     /locus_tag="PKV56_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          896 a         1618 c         1710 g          973 t
ORIGIN      
        1 cggtggcgcg gtcgagccag cggttggccg ccaccgcctg caccagggaa tggaagtcgt
       61 ggttggcgcg gtagtagccg tcgacatcgg ccgcggcggc ggcgcgttcc agcttgtcgt
      121 gcagggcctg cagctgggcc agatcgtcga cggtggcgcg gcaggtggcc tccagcgcac
      181 agcggccttc cagcatcgcc atcaccggaa acagctgctc ggcgtcggca tcggtcagcg
      241 ccacgacgcg ggcgccgcgc cggggcacca gttcgaccag gccttcggcg gccagcacct
      301 tcagcgcctc gcgcagcggc gtgcggctga tctgccagcg cgcggccagc gccagctcgt
      361 cgatgaacag gccgggcgcc agttcatgct ggaacaccag ctcgcgcagc cgggcggcca
      421 cgccgtcgtg caggctggtg ccgtgcaggg ggatgggatc gggcatgccg catcgtaatg
      481 ctgtaattac agaatgacaa gccggaaggc aaggacggtg ctcgacccat cggccagcat
      541 ggcctgagcg gccggatccg cagcgggtca ccggaggctg tcctgcgcct gatggccgag
      601 gccgggctcc cgctcagtcc gagatcaggt tgacgatgcg acgtggcgcc tgcgcctcca
      661 gccggtcgcg caggaagccg aaggcgaagt caatttcctc ctgcggcacg gcgggatgca
      721 ggccggggtt ggcatgcagg cgcttgtcgg cactgcccag cgcgtcaaac agggccaggc
      781 agccctcgcg gccaaagagt tcgtcctcca actgcaccaa gaacaccact gggcactgga
      841 tgcgtgccgc atcggccagc agccgcgtgc ccaggtgcgc cgccgccccc tggctgccca
      901 ggaggcccag cgtggccaca gtcacatcgt tgcgtgcagc cagcagcgga atgccgtaga
      961 tcgagcccat cgacagcccg aagtacgcca gccgcgcgcc acgcaagccc ggcacggcgc
     1021 gcgcggcgtc aatggccacc gcccaatcgg ccaccatgtc gtcaatgctg cccggccgct
     1081 ggatttcggg aaagaacgcc tcgcgcccac ccgcacccac ctggcgcaag ccatgcaccg
     1141 ggccgtccag cgacagcagc gcgcagccag cctcctgcgt gaaccgctgc gccaggctgg
     1201 caatgggcgc ctggtagcgg ttgcccgacg cgccatggcc gcacagcacc agcggctggt
     1261 cgggccgcgg ctgcgcgggc agccacagcg caccggtgac gctgcgccca ccggcgcggg
     1321 tcagtcggaa ttcacgcgtg gcgtggccgc gggggtcggt ggcgtcttgc caatgcacgg
     1381 gactggcctc ccgggtgtgt ggggttgaac cgttcatgca acggcattgt ggtgggccat
     1441 gacccgctgc acagtcggcc gaacccgact ttgaagggca agccgctcga tacggccacc
     1501 gcaggggcag gcggtaccca gcggtgcaga gtgagtgacg cgtccgacgg gtgaaagacc
     1561 tggtggatgc cgctggacag ctggccgaaa acagacagcc accgcaacca gggcgcgtgc
     1621 ggatgttcag ccctgcggcc agtctggctg cccgtgtgca aggcatcgga cacctggccc
     1681 atgggcccgg ccatcaacgg agcggctcga ttggaggtgg catcaccact ctgcaacctc
     1741 gggtgtggac gactgatgag tgaacttcat tgggtggttc actaagctct gtcggatatc
     1801 tttacagagg cgatcattta ggagcgacta gttcaacaaa atcaagacct tgcgaggctg
     1861 gcgcgggatt tgctagcggc gaacttgagc gtgcgcgggg cgaggccccc agcgtgcccg
     1921 tcgcctgtta aggtcgttca atgagggaga gaatgcatga agaaggtctt tgcgatgttt
     1981 gcgctgacgg cagccatgcc gttggctttt ggcgcggtag ttaacctcaa catcatccaa
     2041 gtctgtgacg atgcaggttt gagctgtgcg gcagtgaact tgaatcagtc cttcacggat
     2101 aagatttggg cacagactgg aaccactttc aattacggct caatcacgca gttgaacagc
     2161 agcaagtatc tgaatccgac cgaggccgaa gcgggatctc tgatcagctc cttctttggg
     2221 actggtcgcg aattttttgc ctatgatgtc tggttcgtga atgggtttgt cggcgccccg
     2281 gggctgcgcg ggttgggcgc attgggcggg gacgggttgg ttgtgtcgag cagtactgcg
     2341 gcagttgata cattggctca cgaacttggg cacaattttg gtttggacca caccgatgct
     2401 gccgtcctga acgcggatcg ctacctgatg gcgtctggag gcattcgtac gatacctacc
     2461 actgttgcca atgtggcgcc tgacgggctt caattgagtc gcttgaatcc gatcttgccc
     2521 aacgtgacgg tggacatgat tggcgccact ccgtttgaga gcagcgactt tttcgatgtg
     2581 aagtatctgg cgggtgcagc aagtgatttg ggtctccgat cattgacgat caaccttgcg
     2641 ccggccaatg cgttcttcga catcaccgat tttgaaccgg gaaccgcagg ctcacctttc
     2701 agctacgggc gtttggatgg tgtaagtgcc tcggacattg cgatttcggg gctcaccgat
     2761 ggaagttcac tgttgacgat gaggtttgcc gaggactcat tcacgtccgg agattcaatt
     2821 gcctttggtg ttgacatgga cttgttcagc aacatcgacg ggttcggcgc cactgcggac
     2881 gagttgcgtt tagccgaggt gacgttgacc ttcgaagatg ggcactcggt cgcgggagat
     2941 ctttcaacgc tgatattcgc ctcggaattt gatcccaacc gaaacctgga tgtcttgaca
     3001 acccggttgg ccgacccgat cagtgccgtg ccagcgcccc catctgtctt gcttttcggg
     3061 ttcggcctgg ctttgctcgg ggcccttcgg tggcgccccg tcaggcagtt ttcgtgacaa
     3121 ttgccgcgca cgctttggca ctggccgctg gagcgtgtgt tctggccaca tcggcgtgcg
     3181 cgaatggtgg cgcgcagcca ggtgccacgg tggatcggcg cagtggtgtc actgctggtc
     3241 ccgactggat tccgtgtata cgtgatccga atcgactgtc gctgaaacac tgttccgaag
     3301 aatacaggct ctgcagagcg gccgggagca gttcaagtgc atgcctcagt ttggagtaca
     3361 aaagcttgac gccagcgtca aaaccgtcga tcgatgagta caccgcctgt cgatcggcag
     3421 ggcacagcgc atcaagatgc aatgatctcc tgggggtgcc cggtcaacgg caatagtgcg
     3481 acggacactg gcttcttatc gccgcagcgt gagggttctc ttcttgggtg acgtgtgaga
     3541 ttgacggcgt gggtcaattg cttcgcgttg cggtttgcga gttcaccact atggcttgga
     3601 atggcaaatc gcccatcctg tgcttgatca ctgcggtcga cggtggttgg accggctcca
     3661 tgtcgtttcg tgtggaaccc aacagtctca tttggcggtc gccaggctga agggatgagc
     3721 ctgcagatgc gcgagcttgg acatgcgcag gtaggcgatg gcgatgaagt tgcttgccgt
     3781 tcggaatccc ctggctgcgc gtttggcctg ctgcagcaag ccgttcatgg cttcgacgaa
     3841 ggcgttgctg cggtggtcgg tcatgccgcg caccacggcg tcgaagcggg ccttgatggt
     3901 ggtggccagc ttcttgaacg gctcgagccg acagcgccga gcccagctga gccaggcgcg
     3961 cagatcgacg ctggcctgct cggcgttgtt gtgcccgcgg gctgcggcgt agacggcctg
     4021 caaggccatc ttcagtcgcc aggcccgcgc gctcttgagt gcggactgct gcagccagtg
     4081 catggcgttg atctgtggct tgctccatcc cgacgggttg cgtcgcatgc cccacatcag
     4141 ctgcttgagc atcttgcggt cgttggcccc cagcgccaag cgcaccgccg cggcgtcgtc
     4201 ctgcatctcc gcacggcgca cctgatccat cgcctcgttg gccatcgcca cgacgtggaa
     4261 ccggtcgtag ctgatctggg cctgcggcag tgcctggccc acacccttgg catacgccgc
     4321 actcatgtcc atgcacgcat ggcgcacctc ggcggggtca ccgccgtggg ccttgagatc
     4381 tgcagcgaag tcgagcacca tctggtggtc tcgcccctcg gtggcaaaca gcaaccgctt
     4441 ggcatcgagg tcgtgcacga cggtgatgta gttctgacca cggcgcaggc tggtctcgtc
     4501 gatgcccacg gtctgcacgc cagcgaagtt ctccagggca cgggcctggt cgacgtagaa
     4561 cgcgatgcgt cgccacagtc gcttgtccac gcagcgcagc aagctggctg cctggcgcac
     4621 cggcaagtcc cggcacagtg tcagggccag cgcctcgaag gccgcagtga agcctgatcc
     4681 cgggcgagcc cacggcacgc tcgcctgcgt ggtcttgccg cagccgccac aggcgatgcg
     4741 cggcacatcg cagtgaagcc aggcctcgaa ctggaagaag tccaggtggc gccacgagcg
     4801 gcgccgccgg tcatgaaccg actaagcgcc agcgccgcag gccgggcagg ccagggcgcc
     4861 gcctcggcag ccgatctcga aatcgatgcg gcgcttggcg gtgtcgagct tgacgtcctc
     4921 caccacccac ggcaactgca gaccccgcgc gctggtgaac agcgcttcaa ccccaacact
     4981 catcgcttcc ttcggctgaa caccgcccgg tggacatgtg gacaggccgc acagcgacct
     5041 gcccacatat ccaccgggct cgactaccac cgggcatcgt ggatgtctct ttccacacga
     5101 aacgacaagg ggccccatca acccgcctcg ctggtcctgc cctgccgccg ccggtagatg
     5161 gtgttgcggc tgatgcccag cgccttggcg gtggccg
//
LOCUS       DAOORV010000226         5167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19350_length_5167_cov_9.454812, whole genome shotgun sequence.
ACCESSION   DAOORV010000226 DAOORV010000000
VERSION     DAOORV010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19350_length_5167_cov_9.454812"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKV56_17740"
     CDS             complement(<1..301)
                     /locus_tag="PKV56_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOX69697.1"
                     /translation="MSVSVTPKAARQIRKALDQRGGGYGLRVAVKTSGCSGYAYALEF
                     AEAPSPDDLSFESEGVCLLVDAKSLPMVDGTQLDWVREGLNEGFKFNNPNATASCG"
     gene            complement(322..741)
                     /gene="erpA"
                     /locus_tag="PKV56_17745"
     CDS             complement(322..741)
                     /gene="erpA"
                     /locus_tag="PKV56_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOX69698.1"
                     /translation="MSACQSQPNAGSSCSTGTAPPAVPFTLPEGASPITVSDAVVNKV
                     GEMLSEEGDPNLKLRIFVTGGGCSGFQYGFAFDDLTKEDDLCVEVGAIKVLVDASSLQ
                     YVMGAQIDYEENIEGSRFVIKNPNAATTCGCGSSFSV"
     gene            complement(773..1201)
                     /gene="iscU"
                     /locus_tag="PKV56_17750"
     CDS             complement(773..1201)
                     /gene="iscU"
                     /locus_tag="PKV56_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOX69699.1"
                     /translation="MAYSEKVIEHYENPRNVGGFTAEDADVGTGMVGAPACGDVMKLQ
                     IKVNPDTGLIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQALAIKNTSIAEELALPP
                     VKIHCSILAEDAIKAAVADYRQRHDATLEQTACKPAHAAA"
     gene            complement(1225..2448)
                     /locus_tag="PKV56_17755"
     CDS             complement(1225..2448)
                     /locus_tag="PKV56_17755"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOX69700.1"
                     /translation="MDLKLEGKPIYLDYAATTPVDPRVVHAMVPYLYEQFGNPASRSH
                     AYGWAAEEAVEIAREHVAALIGADPREIVWTSGATESNNLAIKGAAQFHAGKGKHLIT
                     VKTEHKAVLDTMRELERHGFEVSYLDVQSDGLLDIEVLKAAIRPDTILVSVMFVNNEI
                     GVIQDVAAIGALCRSKGILFHVDAAQASGKVAIDLGELPIDLMSLSAHKTYGPKGIGA
                     LYVRRKPRVRIEAQIHGGGHERGMRSGTLPTHQIAGMGEAYRLARESMASENERIGKL
                     RDRLLAGAKKIPEVLVNGHLTQRVPHNLNLSFTFVEGESLLMGMKGIAVSSGSACTSA
                     SLEPSYVLRALGLSDEVAHSSIRFSIGRYTTEAEIDAALARVQATVERLRELSPLWDM
                     HCAGVDLSTVQWAEH"
     gene            2799..3305
                     /locus_tag="PKV56_17760"
     CDS             2799..3305
                     /locus_tag="PKV56_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69701.1"
                     /translation="MNLFTGEAADAFFGGDMPESARRLLHQAARSARGEVGALLWTAQ
                     AIAPKALGIYYALYKHHAGLREFEQAERAALRGLSEAAKQAGLDEDWRQVTPERVPDG
                     VNFRGSGAARFWLFTLKALAFIALRSGRPEDARELLAHIAALDDQAHLGSEVIATLLA
                     SITSGSGG"
     gene            complement(3325..3687)
                     /locus_tag="PKV56_17765"
     CDS             complement(3325..3687)
                     /locus_tag="PKV56_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69702.1"
                     /translation="MPPMITAPMLQLTSDAAEAVLILTDGLQRGDLLRSRLTRAEVQR
                     QLQCLADTLEDAPDVLRHQMPEIDWAGWRATRLALATPGAQHDDALWFAVQSLVPATL
                     SWLRVYREQQPSLFQSWH"
     gene            3738..4340
                     /locus_tag="PKV56_17770"
     CDS             3738..4340
                     /locus_tag="PKV56_17770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOX69703.1"
                     /translation="MKRMLLTERLRLRAPQPGDELAAFDGWARDPEVLRYLGWRPHRA
                     IADTRAQLDWDQARWLKRSAWTWLLIQQATGRHTSAEPGDSAPHPRSTAIGIVQLIPQ
                     RFDGPVHHLRLGCLLTRSRWGQGLMREAVSAVLAEAFEQASVWRVDALCDVDNHASAR
                     LFDALGFEHEGVLRRHSLHPNVGDEPRDVTVHARLRSPGG"
     gene            complement(4342..4761)
                     /locus_tag="PKV56_17775"
     CDS             complement(4342..4761)
                     /locus_tag="PKV56_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOX69704.1"
                     /translation="MKVSARNVFEGQISALVPGPINAEVTLTLAGGDQLVAVITQASA
                     RDLELAVGGRACAIIKAPWVIVTVDGGPRLSSRNQLRGVVSAVKAGPINAEVAITLPG
                     GAVVHAVVTQDAVSELGLAPGVPACAVIKASHVVLGV"
     gene            4967..>5167
                     /locus_tag="PKV56_17780"
     CDS             4967..>5167
                     /locus_tag="PKV56_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX69705.1"
                     /translation="MKPRQVTPRLTGHLEVSTDLGAFLGDKRVRLLEAIDLYGSISQA
                     AKHVPLSYKAAWDAVDAMNNLAD"
BASE COUNT          820 a         1723 c         1729 g          895 t
ORIGIN      
        1 agccgcagct cgcggtggcg ttcgggttgt tgaacttgaa gccctcgttc aggccctcgc
       61 gcacccagtc gagctgcgtg ccgtcgacca tcggcaggct cttggcatcc accagcaggc
      121 agacgccctc gctctcgaag ctgaggtcgt cgggggaagg tgcctccgcg aactccagcg
      181 cgtaggcgta gccggaacag ccgctggtct tgacggcaac acgcaagcca tatccgccgc
      241 cgcgttgatc gagcgccttg cggatctggc gggcggcctt gggggtgacg ctgacggaca
      301 tgatggactc ctgtggacgg ttcaaaccga gaacgaactg ccgcaaccgc aggtggtggc
      361 tgcgttcggg ttcttgatga cgaagcgcga accttcgatg ttctcctcgt agtcgatctg
      421 cgcgcccatc acgtactgca ggctcgacgc atcgacgagc accttgatgg cgccgacctc
      481 gacgcacagg tcgtcttcct tcgtcaggtc atcgaaggcg aatccgtact ggaagccgga
      541 gcagccgccg ccggtcacga agatgcgcag cttcaggttg gggtcgccct cctcgctcag
      601 catctcgccg accttgttga cgactgcatc actcacggtg atgggcgagg cgccttccgg
      661 cagcgtgaag ggaacggcgg gtggcgcggt gccggtcgaa caggaagaac cggcattggg
      721 ctgtgattgg catgcggaca tgggaggctc ctggttcagt tgggggcaag gttcatgcgg
      781 ccgcgtgtgc cggcttgcat gcggtttgtt ccagcgtggc gtcgtgccgc tggcggtaat
      841 cggcgacggc cgccttgatg gcgtcctcgg caaggatcga gcagtggatc ttcacggggg
      901 gcagcgccag ttcctcggcg atgctggtgt tcttgatcgc cagcgcctgg tccagcgtct
      961 tgcccttgac ccattcggtg accagcgagc tcgaggcgat cgccgagccg caaccgtagg
     1021 tcttgaactt cgcgtcctcg atcaggccgg tgtcggggtt gaccttgatc tgcaacttca
     1081 tgacgtcgcc gcaggcgggc gcaccgacca tgccggtgcc gacgtcggca tcttccgcgg
     1141 tgaagccgcc gacgttgcgc gggttctcgt aatgctcgat gactttttcg ctgtaggcca
     1201 tgatgggctc cgtggtgttt cggttcagtg ctcagcccac tggacggtgc tgaggtcgac
     1261 gccggcgcag tgcatgtccc acagcgggct gagttcgcgc aggcgttcga cggtggcctg
     1321 gacgcgcgcc agagcggcgt cgatctcggc ttccgtcgtg tagcggccga tcgagaagcg
     1381 gatcgagctg tgcgccactt cgtccgagag gccgagcgcg cgcagcacat agctcggctc
     1441 gaggctggcc gaggtgcagg ccgaacccga cgacaccgca atgcccttca tgcccatcag
     1501 cagcgactcg ccttcgacga aggtgaagct caggttgagg ttgtgcggca cgcgctgcgt
     1561 gaggtgtccg ttgacgagca cctcgggaat cttcttcgca ccagccagca gccggtcgcg
     1621 cagcttgccg atgcgctcgt tctcgctggc catcgactcg cgcgccagcc gataggcctc
     1681 gcccatcccg gcgatctggt gggtcggcag cgtgcccgag cgcatgccgc gctcgtggcc
     1741 gccgccatgg atctgcgcct cgatgcgcac ccgcggcttg cggcgcacgt acagcgcacc
     1801 gatgcccttg ggtccgtagg tcttgtgcgc cgacaggctc atcaggtcga tcggcagttc
     1861 gccgaggtcg atcgcgacct tgccgctggc ctgggccgca tcgacgtgga agaggatgcc
     1921 cttgctgcgg cacagcgcac cgatggcggc cacgtcctgg atcacgccga tctcgttgtt
     1981 gacgaacatc accgacacca ggatggtgtc cggacggatc gcggccttca gcacctcgat
     2041 gtccagcagg ccgtctgact gcacgtcgag gtagctcacc tcgaagccgt ggcgctcgag
     2101 ttcccgcatc gtgtcgagca cggccttgtg ctcggtcttc acggtgatca ggtgcttgcc
     2161 cttgccggcg tggaactgcg ccgcaccctt gatcgccagg ttgttcgatt cggtcgcgcc
     2221 ggaggtccag acgatttcgc gcgggtcggc accgatcagc gcggcgacgt gttcccgggc
     2281 gatctccacc gcctcctcgg cagcccagcc gtaggcgtgg ctgcgggagg ccgggttgcc
     2341 gaactgctcg tacaggtagg gaaccatcgc atgcacgacc cgcgggtcca cgggtgtggt
     2401 ggcggcgtag tccagataga tcggtttgcc ttcgagcttc aggtccatcg catgactcct
     2461 ctgtggatcg cccccgcagg ccgccgtgct gcggcaaccc gacctcggct ggggtcaggg
     2521 aacgtcggtt cgattcgacg tttccctgga ggcgggtgct ctaccttctc aaggaacgtg
     2581 ccagtcgatc cgggcaaccc gcgcaatccg gaaaagcgac cgcgcatcaa tcacttggct
     2641 cgcttttgcc cctcgatcca gcgctttcca ggcagcgtgg atccgtgtcg gatcgctgtc
     2701 ggctttgtcg caacttcgac gcgccgcagc gacgttcctc gcaggttgcg ccgggcccgg
     2761 ggcttgcgtt gcctgtgcgc cgcacttcga gacacgacat gaacctgttc actggcgaag
     2821 cagccgacgc cttcttcggc ggagacatgc ccgaatccgc acgccgcctg ctgcaccagg
     2881 cggcgcgctc ggcgcgcggc gaagtgggtg cgctgctgtg gacggcgcag gccatcgcac
     2941 cgaaggccct cggcatctac tacgcgctgt acaagcatca cgcgggtctg cgtgaattcg
     3001 agcaggcgga gcgcgccgcg ctgcgcggac tgagcgaggc ggcgaagcag gccgggctgg
     3061 acgaggactg gcgccaggtc acgcccgaac gcgtgccgga cggcgtcaac ttccgcggca
     3121 gcggagcagc gcgcttctgg ctgttcacgc tcaaggcgct ggccttcatc gcgttgcgca
     3181 gcggacgacc cgaggacgcg cgggaactgc tggcgcacat cgccgccctc gacgaccagg
     3241 cacacctggg cagcgaggtc atcgccacct tgctggccag catcacgagc ggctccggtg
     3301 gttgaacttc cggtgtcgcc ccggtcagtg ccaggactgg aacagcgacg gctgctgctc
     3361 gcgatagacg cgaagccagc tcagggtcgc cggcaccagg gactgcaccg cgaaccacag
     3421 ggcatcgtcg tgctgtgcgc ccggggtggc cagcgccagc cgcgtggcgc gccatcccgc
     3481 ccagtcgatc tcgggcatct ggtgtcgcag cacatcgggc gcgtcttcca gggtgtcggc
     3541 caggcactgc agctgacgct gcacctcggc ccgcgtcagg cgagaacgca acaggtcgcc
     3601 gcgctgcagt ccgtcggtca ggatgagcac cgcctcggcc gcgtcgctcg tcagttgaag
     3661 catcggtgca gtgatcatgg ggggcatcag gatgcacctt tcgcgccagc accatcactg
     3721 gtcggcaggc gccgccgatg aagcggatgt tgctcaccga gagactgcgg ctgcgcgcac
     3781 cgcagcccgg cgacgaactg gcggcgttcg atggctgggc gcgggacccc gaggtgctgc
     3841 gctacctggg ctggcgtccg caccgtgcca tcgcggacac gcgggcgcag ctggactggg
     3901 accaggcgcg ctggctcaag cgcagcgcgt ggacctggct gctgatccag caggcaacgg
     3961 gccgccacac ctcggccgag ccgggtgact cggcaccgca tccccgcagc actgcgatcg
     4021 ggatcgtcca gctgattccg cagcggttcg acggcccggt ccaccacctg cgcctgggct
     4081 gcctgctgac gcgcagccgc tggggccagg gcctgatgcg cgaagctgtc tcggcggtgc
     4141 tggccgaggc cttcgaacag gcatcggtgt ggcgcgtcga cgcgctgtgc gacgtggaca
     4201 accacgcttc ggcacgcctg ttcgacgcgc tgggcttcga gcacgaaggc gtgctgcgcc
     4261 gccacagcct gcatccgaat gtcggcgacg aaccgcgcga cgtcacggtc cacgcccgcc
     4321 tgcgctcccc gggcggatga atcagacgcc gagcacgacg tgactggcct tgatcaccgc
     4381 gcaggcaggc acgcccggcg ccagcccgag ttccgacacg gcatcctgtg tgaccaccgc
     4441 atggacgaca gcgcctccgg gcagcgtgat ggcgacttcg gcgttgatcg ggccggcctt
     4501 cacggcgctg acgacaccgc gcaactggtt gcgagaggac aggcgcggtc ccccatcgac
     4561 ggtcacgatg acccacggcg ccttgatgat cgcgcaggca cgacccccga ccgccagctc
     4621 cagatcgcgc gcgctggcct gcgtgatcac cgcgacaagc tggtctccgc cggccagcgt
     4681 cagcgtgacc tcggcgttga tcgggccggg gacgagtgcc gagatctgac cttcgaagac
     4741 gttgcgtgca ctgactttca tctgagtggc tcctggaggt tcgatggatg gcacaccgct
     4801 tgcgttatat cttcaaagat actacgcatg tcggttaccg gacaatccaa ccttacgact
     4861 catcgtccat gcaaagaaac catagcgctc gtcaggatct tgacatccca tgtgccgatc
     4921 gaacgaccat gatccatcgc tatagctcga ttgacacaac ccattcatga aaccacgcca
     4981 agtcacgccg cgcctgacgg ggcacctcga agtcagcacc gacctgggcg ccttcctcgg
     5041 cgacaagcgc gtgcggctgc tcgaggccat cgacctgtac ggatcgatct cgcaggcagc
     5101 caagcacgtg ccgctgtcgt acaaggccgc ctgggacgcg gtcgacgcga tgaacaacct
     5161 ggccgac
//
LOCUS       DAOORV010000227         5161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19382_length_5161_cov_13.529965, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000227 DAOORV010000000
VERSION     DAOORV010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19382_length_5161_cov_13.529965"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PKV56_17785"
     CDS             complement(<1..120)
                     /locus_tag="PKV56_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX69706.1"
                     /translation="MNTSVFILQLLNGVQYGVLLFLLSAGLTLVLGIMGFVNLM"
     gene            complement(152..1330)
                     /locus_tag="PKV56_17790"
     CDS             complement(152..1330)
                     /locus_tag="PKV56_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX69707.1"
                     /translation="MKRFLPIAPRATLAAIVLTCAQAFAQAPVKIGFVAELSGPQAAL
                     GQDQYDGFMLYVEQQGGRLGGVPVQVLREDTQLKPEVAVQAVHKLIEREGVPIITGVT
                     FSNIMMAIHKPVTERQVFLISSLSGPSPIAGAQCSPYQFVTSFQLDAAAEAHASYANQ
                     KGYKRVFLLAPNYQSGKDFLTGFKRVFKGEVVDEVYTAMGAPDYSAELTQIAAAKPDA
                     VFAFYPGAMGINFVRQYGQMGLLKSTPLLSQGAADGSTLPALGEAALGAFNGAPWGPD
                     LDNPANRRFVQAYEQKYRRIPTLFAAQSYDAAQLLDSALRKVKGNVQDKDALRQALKA
                     ADFPSVRGKFRFGNNQFPVQDYYIQQVARDAQGRVNLKTVSTPLKDFVDAYASQCPMK
                     "
     gene            1504..2091
                     /locus_tag="PKV56_17795"
     CDS             1504..2091
                     /locus_tag="PKV56_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX69708.1"
                     /translation="MSTERSPRRAPAPATPARPPSDTTDRQLSDRLTDSLGFLIVDSG
                     RFVKRSLYARIADHGIRGGAWFVLRVLWQRDGLTQRELAGRLGLMEPSVQEMLRAMER
                     DGLVTRQRDEQDRRKVRIHVTDRALELKPALMGIADEVNTLMLSGLDAAEEALLKLLL
                     RKVRETLAADADALATALPEEPVPGARTTRGRASR"
     gene            2283..2819
                     /locus_tag="PKV56_17800"
                     /pseudo
     CDS             2283..2819
                     /locus_tag="PKV56_17800"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(2803..3668)
                     /locus_tag="PKV56_17805"
                     /pseudo
     CDS             complement(2803..3668)
                     /locus_tag="PKV56_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4055..4330
                     /locus_tag="PKV56_17810"
     CDS             4055..4330
                     /locus_tag="PKV56_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX69709.1"
                     /translation="MADDKRAPRRLDSGEFKAQVLAEFDVSGPSVAKVAMAHGINESV
                     LHGWRKLARERGAATLPRPSEFVPVTVTAALTQMTSAASKWNWAAAR"
     gene            4414..4826
                     /locus_tag="PKV56_17815"
                     /pseudo
     CDS             4414..4826
                     /locus_tag="PKV56_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612183.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4870..>5161)
                     /locus_tag="PKV56_17820"
     CDS             complement(4870..>5161)
                     /locus_tag="PKV56_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69710.1"
                     /translation="RRSRHLSSCVQQTPGHRQSVARSSAARFEGFVNASVLAEALRRA
                     GPALSTDAFIRALESAEPIRFGRFGLTYTSSAHPGSTYVELAIIDESGQLRY"
BASE COUNT          926 a         1660 c         1681 g          894 t
ORIGIN      
        1 catcaggttg acgaagccca taatgcccag caccagcgtc aagcccgccg acagcaggaa
       61 cagcaagacg ccatactgca cgccgttgag cagctgaaga atgaagaccg aagtgttcat
      121 gatctgacgc gctggacgct gggccgcccg ttcacttcat cgggcactgc gaggcatagg
      181 catcgacgaa gtccttgagc ggcgtcgaca cagtcttgag gttgacccgg ccctgggcgt
      241 ccctggcaac ctgctggatg tagtagtcct gcaccgggaa ctggttgttg ccgaagcgga
      301 actttccgcg caccgacgga aagtccgccg ccttgagcgc ttggcgcaac gcgtccttgt
      361 cctgcacatt gcccttgacc ttgcgcagcg ccgagtccag cagctgcgcc gcgtcgtagc
      421 tctgcgccgc gaacagggtg gggatgcgcc tgtacttctg ctcataggcc tgcacgaagc
      481 gccggttggc ggggttgtcc aggtccgggc cccacggtgc gccgttgaag gcgccgagcg
      541 ccgcctcgcc gagcgccggc aacgtcgagc cgtccgccgc gccctgcgac agcagcggcg
      601 tgcttttgag caggcccatc tgaccgtact ggcgcacaaa gttgatgccc atcgcaccgg
      661 gatagaaggc aaacacggcg tcaggcttgg cagcggcgat ctgcgtcagc tcggccgagt
      721 agtcgggcgc gcccatggcg gtatagacct cgtcgaccac ctcgcccttg aagacccgct
      781 tgaagccggt caggaagtcc ttgcccgact ggtagttggg cgccagcagg aagacccgct
      841 tgtagccctt ctggttggca tagctggcat gggcctcagc ggcggcgtcg agctggaacg
      901 atgtaacgaa ctgatacggc gagcactgcg cgccggcgat cggtgacggg cccgatagcg
      961 agctgatcag gaacacctgg cgctcggtga cgggcttgtg gatggccatc atgatgttgg
     1021 agaacgtgac cccggtgatg atgggcacgc cctcgcgctc gatgagcttg tggaccgcct
     1081 gcacggccac ctcgggcttg agctgggtgt cttcgcgcag cacctggacc ggcacaccgc
     1141 ccagacggcc gccctgctgt tcgacgtaga gcatgaagcc gtcgtactgg tcctggccga
     1201 gcgctgcctg gggaccggac agctcggcga cgaagccgat cttgaccggc gcctgcgcaa
     1261 aggcctgcgc gcacgtcagg acgatggctg caagggttgc gcggggtgca atggggagga
     1321 atcgtttcac gaggcgtctc ctgtgcagtg aggggtcggt gtctcgaatt tttaccaagg
     1381 aaccttagta tttttgcaaa ggatccttag tgagtgcaag ccacgaaacc taggcataaa
     1441 cccgaatctt caccggtata gggctggcct ggagccacag cgaacgatta gcatccttcc
     1501 catatgtcga ccgaacgctc ccctagaagg gcgccagcgc ctgccacacc ggcgcgcccg
     1561 cccagcgaca cgactgaccg gcaactgtcg gacaggctca ccgacagcct gggcttcctg
     1621 atcgtggaca gtggccgctt cgtcaagcgt tcgctctatg cgcgaatcgc ggaccacggc
     1681 atccggggtg gtgcatggtt cgtgctgcgt gtgttgtggc agcgtgacgg cctgactcag
     1741 cgcgagctcg ccggcaggct gggtctgatg gagccctcag tgcaggaaat gttgcgcgcc
     1801 atggagcgcg acggcctcgt gacacgccag cgcgacgaac aggaccggcg caaggtgcgc
     1861 atccatgtca ccgatcgagc cctggaactg aagcccgcgc tgatgggcat tgccgacgaa
     1921 gtcaacacgc tgatgctgag cggcctcgat gcggccgagg aagctctgct caagctgctg
     1981 ttgcgcaagg tgcgcgagac cctggccgcg gacgccgatg cactggccac ggccctgcca
     2041 gaagaacccg tgccgggcgc gcgcactacg cgcgggcgag catccagatg aggagcacct
     2101 cttgtccttc cgcggccaag cgccgctgcg acgctcacgg acgcccatcc cagactgaac
     2161 tactccatga cccagtccac gccagttggt tcaaccagat cgaaatctac ttttcggtcg
     2221 tacaacgcaa gtcctgatta gcagcgaagc tcagggggtg gctcacctgg tgagactgct
     2281 gtgagtgcgt ggcctgcggc taccagtgca gcctcatcgc cgggacgatg ttcatggcca
     2341 tgcagctcat cagccaggcc aagaacatcg tcgcggcgct ggagctgcgc cgccagctcg
     2401 gggtgtcgta cccaacggcc tggctgatca agcacaagtt caccgaggcc atgcggctga
     2461 cggagcagcc ccgccagctc cacggccggg tggaagtcga cgacgcctac ctgggcggcc
     2521 agcgctcggg cggcaaggcc gggcgcggct cggagaacaa agtccctttc gtgctggccg
     2581 tgcagaccac cggtgagatc aagccgcacc aggcctgcac ggcgcagctt cccttccgcc
     2641 gcggccatat cgccgagttc tgtggccggc atctcgcgcg tccgctgacc gtggtctccg
     2701 acgggctgga ctgcttccgc gccaccgccg agaccggcgt gcatgagcgc atcgtcaccg
     2761 gcggcggggc cagcagcgca cgtcatcttg gacctcggcc acgaactcgg cgctgctgaa
     2821 ctgactgccc tggtcggtgt tcacgatctc cggcgctccc cagcgcgcca ccgcttcgcg
     2881 caaggcctcc acgcagaagt ccgcctccat cgtgatcgac acccggtgcg cgagcacccg
     2941 gcggctggcc cagtccagca ccgccacgag gtggacccag ccacgagcca tggggatgta
     3001 cgtgatgtcc agcgcccaga cctggttggc gcgctcgatg ctcagcccgc gcaaggcgta
     3061 cgggaacacc gggtgcgccg ggtgcttcct gctcgtgttt agcttgcggt acagcgcctg
     3121 gatgcccatg cgccgcatca gcgtgctcac gtgccgccgg cccacctgca ggccttgcag
     3181 gcgcagcagg tcgcgcagca tgcggctgcc cgcgatcggg tgctccaggt gcagttcgtc
     3241 gatgcgccgc atcagcgcca gctcggtctc ggcggtcggc tcggggtggt agtacaccgt
     3301 gcctcggctg atgcccagca gctgcgcctg ccacttgatc ggcagtcggg cttcacggtc
     3361 ggtcatcgct ttgcgctcag caatcccgcc ttggtgagcg cgcgctacaa ataatcgttc
     3421 tccagcgtca gctgcccgat cctggcgtgc agtgccttca gatccaccgg cgccgtctcg
     3481 ggcgctacag cgccgccgaa tacgcccgcg gcgcgctcca tcagttgccg gcgccactcg
     3541 ggctcctggt tggggtgcac atcgtgcttc tgcgcgatct cggcaatcgt cttgtcctcg
     3601 gccaaagcct cgatcgccac cttggctttg aacgccgcag agtggttgcg gcgcggtctt
     3661 ctgctcatct cctgtgtgct ccttcgtctc ggctctatcc tcgcccttcg gagcagccag
     3721 tccaccttag gccgtgttca gattcgcgcg gccggctccc agatcgatgc cctgtcccga
     3781 gcatgcgatt cgggcacctg agtttgcttg ctaatcagga cgtaagttgc tgactcacga
     3841 cgacttccta tgcctcgaat cggtggcagg cggttggtgg aacgctctga gctccacttc
     3901 aatagcaaca cccgtccatt cgaggcctga cttgcccggc tgcttcgggc caatcaatcc
     3961 cgtgaagatg ggtcgtgagc gcgcggcgat cacatcttga ggtcgacgcc gacgacgttg
     4021 gcgatggtgt ccgcgtaaga cctaagtgga cacgatggcg gacgacaaga gagcgccccg
     4081 gcggctcgac agcggggagt tcaaggcaca ggtgctggcc gaattcgatg tctccgggcc
     4141 gtcggtcgcc aaggtggcga tggcgcacgg catcaacgaa agcgtcttgc acggatggcg
     4201 caagctggcg cgcgaacgcg gtgctgcgac gcttccgcga ccaagtgagt tcgtgccggt
     4261 gacggtgact gcagcgctca cacagatgac gtccgcggca tcgaaatgga actgggccgc
     4321 ggctcggtga cgatgaagat caccctgacc tgcccccctc aagccgtacc aaggtagagg
     4381 aagtgaagtc cgctcatcag gagagtggcg gacatgagaa cgagcaagtg cagcgaggaa
     4441 caggtcatcg ggttcctcaa gcaggccgat gccgggctgc cggtcaagga tctgtcccgc
     4501 aagcacggtt tcagtgagcc aagctactac ctctggaagg ccaagtttgg cggaatgaat
     4561 gtgtcggacg cgcagcgcct gaaggcgctg gaggccgaga acacgaagct caagaagttg
     4621 ctggtcaact cgatgctcga gatcgacgcg atgtgcgagg tgctcaaggc aaagtgacgg
     4681 tcgtggcggc gcgtcgcgag gtcgtgaagc aattgcaggg actgggcttg agcgaacgcc
     4741 gggccttgag actggtaagc atgagcgcca gcaccctgcg ctaccgaccg cgcgacgacg
     4801 gcaacggccg gctgcgcgaa cgtctgacgg agcgcagctg tgtcaccgcc atgcgcacgc
     4861 gcttggggat cagtagcgca actgtccaga ctcgtcgatg atggccaact cgacgtaggt
     4921 ggaaccgggg tgcgcgcttg atgtgtacgt caacccaaaa cgcccgaagc gtataggttc
     4981 tgcgctctcc aacgcacgga tgaacgcgtc tgtagacaat gccggaccgg cgcgacgcag
     5041 ggcttctgcc aacacactcg cattgacaaa gccctcaaac cgcgcggccg acgaccgggc
     5101 gacgctctgg cgatgtcccg gagtctgttg aacgcatgag ctgaggtggc ggctccttcg
     5161 a
//
LOCUS       DAOORV010000228         5133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19521_length_5133_cov_7.117566, whole genome shotgun sequence.
ACCESSION   DAOORV010000228 DAOORV010000000
VERSION     DAOORV010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19521_length_5133_cov_7.117566"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..826
                     /locus_tag="PKV56_17825"
     CDS             131..826
                     /locus_tag="PKV56_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOX69711.1"
                     /translation="MEPLPPALTELPLFPLHSVLFPGGYLPLRIFEVRYLDMIGRSHK
                     AGTPFGVVCLIEGREVRRAPPLSSAPPDAPGDGFATESFHDIGTLATITQLERPNPGL
                     LTICCTGTQRFRIHERERLKHGLWMAQVELLPDDAVVAVPSDLETTVTTLQCVVRKLA
                     DRADTTAEMPLQPPYRWDDCGWVANRWCELLPLGEADKQRFMSLDNPLLRLELVSDVL
                     DQTGLAQSLSSKR"
     gene            866..1285
                     /locus_tag="PKV56_17830"
     CDS             866..1285
                     /locus_tag="PKV56_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOX69712.1"
                     /translation="MARPADPQKFAVCVQPSRIDGQGAFAAEAIPARRKIGEIRGESV
                     SVKVARQRARTQQRIMIVEVSERRAIDASQSTDPLRYTNHSCQPNAVLRIRQGRVELY
                     AMRDVALGEELTVNYGETHHEGRLRCRCGAAGCVGAL"
     gene            complement(1296..1976)
                     /locus_tag="PKV56_17835"
     CDS             complement(1296..1976)
                     /locus_tag="PKV56_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX69713.1"
                     /translation="MPKSYLEPFWHAVTRLGEAQILLPTAVALALWLAWRARAQRLAA
                     VWLLCIAVAAAVTTASKVAFMGFGWGWPALDFTGVSGHAMFASAILPVVCLALACGPE
                     GRTSATWQRAGLAVGVGLALLVGVSRAAIGVHSWSEIVAGWLVGGVASAALLRAVPMP
                     SARLPLWLPVAVGLWLGTASAKAPPSATHGLVTRWALALSDRSQPYTRVDMLTAWQLE
                     QQRARISR"
     gene            2217..2654
                     /locus_tag="PKV56_17840"
     CDS             2217..2654
                     /locus_tag="PKV56_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69714.1"
                     /translation="MNLFGWLDRGGPSAPAHRPRTASRPTSQPANADWARVRQPQRPQ
                     DLVLSAMAGHWRTSLADRYRPDQLCTLYPRLANRLALCWDDPSLASKVLDDLVVDKRR
                     GRAGFPPSVSQELIRLRLLRPAPQRPSDFTPLWDPTTMACGDR"
     gene            complement(2661..3833)
                     /locus_tag="PKV56_17845"
     CDS             complement(2661..3833)
                     /locus_tag="PKV56_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69715.1"
                     /translation="MNTDLFANDLFPVDLSAVVHPCAETRAPAALLTTHEHTGFELGW
                     DHAHYGVTPPAPYAQEPSPLRHGWLAGEAAFGPRTLKPTATVRQWLQLRLHAWLRGRS
                     VELFQVTPNYLQRLDVGHCPITREALSGPAGERRDASIDRVRSDAGYAAGNLVMLSSR
                     AQVAKAAHGFEGARAIARRLESDLGRGHGAAVLQDDLSAIHWGRIAVLCSFVEPLPHA
                     QACELPMLVLPPNRLRLFNPVQALQAFISQQLLSAGWSQRINAFEALLSGQPLRRAFR
                     SFFQTLLPRVLEAGRSADPQRVRWAVEDAWRHAPVMQRWVRFALLLTPAKCESLLSRA
                     AVQHLVPAGMRFEPLADADATEGWCLASRGYVPHGLQRQPSTRALAGSARCPALSH"
     gene            4008..5015
                     /locus_tag="PKV56_17850"
     CDS             4008..5015
                     /locus_tag="PKV56_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOX69716.1"
                     /translation="MDRTERFYKIELLLRSRGCVSFDALLAELAVSPATLKRDLQYLR
                     DRMGAPITYDRDDNGYRFDLPTTGAANSSRAMHHELPGMWFSERELHALLTMHQLMAG
                     LDDDGVLARHLQPMMDRLQGLLGADASEARELVRRVKVIGTARRRVPSRHFELLGSAL
                     VQRKRVWLRYFKRSDRVTSEREVSPQRLVNYRNTWYLDAWCHASQGLRRFALDAIEAA
                     RSLDTRARPVALKDLEAELDAGYGIYGGGSKVKWATLVFERDAAQWVAHEEWHPEQKS
                     RWLGGRSGEGSYELVVPYSDPTELMMDILRHGDSVRVAGDKALAAAVAQRLAKAAALY
                     A"
BASE COUNT          917 a         1732 c         1718 g          766 t
ORIGIN      
        1 tcccagccgc agcccgccga caccgtgtgg gcgcaggttg acgagatcaa ggccttctac
       61 ggcatccgcg agcagtgcct ggaaaacctc ttgcgggaca ggcgcagtgc ggccgaacca
      121 caatccacag atggagccgc tgccgcccgc gctcactgag ttgccgctgt ttccgctgca
      181 cagcgtgctg ttcccgggcg ggtacctgcc tttgcggatc ttcgaggtgc ggtacctcga
      241 catgatcggc cgcagccaca aggcgggcac gccattcggg gtggtctgcc tgatcgaagg
      301 ccgggaggtg cgccgggctc cacccctgag cagcgctccg ccggacgcgc ctggcgacgg
      361 tttcgcgacc gagtcgttcc atgacatcgg aacgctcgcc accatcacgc aactggagcg
      421 gccgaacccg gggctcctga caatctgctg caccggcact cagcgttttc gcatccacga
      481 gcgcgagcgc ctgaagcacg ggctgtggat ggcccaggtc gaactgctgc cagacgatgc
      541 ggtggtggcc gtgcccagcg acctcgaaac gacggtgacc acgttgcaat gcgtcgtcag
      601 gaagctcgcc gatcgtgccg acaccacggc cgagatgccg ctgcagccgc cctatcgctg
      661 ggacgattgc ggctgggtcg ccaatcgctg gtgcgagctg cttccgctgg gcgaagcaga
      721 caagcagcgg ttcatgtcgc tcgacaaccc cttgctgcgc ctcgaattgg tctccgacgt
      781 gctcgaccag accggtctgg ctcagtcgct gtccagcaag cgctgacagt cgtcagcgct
      841 agtccgggcc cgcaccaacg tctcgatggc ccgtcctgcc gacccccaga aattcgccgt
      901 ctgcgtgcag ccgagccgca tcgacggaca gggtgcgttc gccgccgaag ccattcctgc
      961 gcgccgcaag atcggcgaga tacgtggcga atcggtcagc gtgaaggtcg cgcggcagcg
     1021 tgcgcgaacg caacagcgca tcatgatcgt ggaggtcagc gagcggcgtg ccatcgatgc
     1081 atcgcaatcg accgacccgt tgcgctacac caaccactcg tgccaaccga acgcggtgct
     1141 gcggatccgg cagggacgcg tcgagctcta cgcgatgcgc gatgtagccc tcggcgagga
     1201 actcaccgtc aattacggag agacccacca cgagggccgc ctgcgctgcc gctgcggcgc
     1261 tgccggctgc gtcggcgcac tctgacacgg cgcaatcagc gagagatcct ggcccgctgt
     1321 tgctccagct gccaggccgt cagcatgtcg acacgcgtgt acggctgact ccggtcggac
     1381 agcgccagtg cccagcgggt gaccagaccg tgcgtggctg agggtggtgc cttggccgat
     1441 gccgtcccga gccacaggcc gaccgcgacc ggcagccaca ggggcagcct tgccgagggc
     1501 atcggtacgg cgcgcagcag cgcagccgag gcaacgccac cgacgagcca accggcaacg
     1561 atttcggacc acgaatgcac accgatcgcc gcgcgcgaga caccgaccag cagtgccagc
     1621 ccgacaccca ccgcgaggcc tgcccgctgc caggtcgcag aggttcgtcc ctccggcccg
     1681 caagccaggg ccaggcagac caccggaagg atcgccgacg cgaacatcgc atggcccgaa
     1741 acgccggtga aatcgagcgc tggccatccc cagccgaaac ccatgaaagc gactttcgac
     1801 gcggtagtca ctgcggctgc gaccgcgatg cacagcagcc agacggcagc cagccgctgc
     1861 gcccgcgccc gccaggccag ccagagcgcc agcgcaaccg cggtaggcaa caggatctgc
     1921 gcctcgccga gccgggtgac cgcgtgccag aagggctcca gatacgactt cggcatgcgg
     1981 cgatgctaag ggaccgatcg tgaccgaacg ccgcgattcg gcgacgcgag tgctgcacca
     2041 tgaaccgtga actgttcaca cctgcaagcc ggggggaaat gccttccggc ctgcactcgg
     2101 gccccagccg gcggtcacac tccgctcatg gaaactttca gttactacct ggcccctgcc
     2161 gaagcggcat cgaacaccgt gcaggtcact ccggagcaca gcggggaggt cacccgatga
     2221 acctgttcgg atggttggat cgcggtggcc cttcggcccc ggcccatcga cctcgcaccg
     2281 cttcgcgtcc gacgtctcag ccggcgaacg cggactgggc gcgtgtgcgg cagccgcaga
     2341 gaccgcagga tctggtgctg tcggcgatgg ctggacactg gcggacttcg ctggcggatc
     2401 ggtatcggcc cgatcaactt tgcacgctgt acccgcgtct ggccaatcga ctggccttgt
     2461 gctgggacga tccgtcgctg gcctcgaagg tgctcgacga tctggtcgtg gacaagcggc
     2521 gcggccgcgc gggcttcccg ccctccgtgt cgcaggagtt gatccgactc cggctgttgc
     2581 gcccggcacc ccagagaccc agcgatttca cgccgctctg ggatccgacg accatggcct
     2641 gcggagaccg ctgagtcgac tcaatgtgac agcgctgggc agcgtgccga gccggcgagc
     2701 gccctcgtcg agggctgccg ctgcagccca tgcggtacat agccccggct ggcaaggcac
     2761 cagccctcgg tcgcatcggc atcggccagc ggctcgaagc gcatgccggc cgggaccaga
     2821 tgctgaacgg ccgcacgcga cagcagtgac tcgcacttcg ccggagtcag cagcagcgcg
     2881 aatcgaaccc agcgctgcat caccggcgcg tgacgccacg catcttcgac cgcccagcgg
     2941 acacgttggg ggtcggcgct gcgacctgcc tcgagcacgc gcggcaacag cgtctggaag
     3001 aacgagcgga acgcccggcg cagcggctgg cccgacagca aggcctcgaa tgcgttgatc
     3061 cgctggctcc agcccgcgct caggagctgc tggctgatga aagcctgcag cgcctgcacc
     3121 ggattgaaca ggcgcagccg gtttggcgga agcaccagca tcggcagctc gcacgcctgt
     3181 gcatgcggca acggctcgac gaagctgcac agcacggcga tgcgccccca atggatcgcg
     3241 ctcaggtcat cttgcagaac cgctgcgcca tggccacgtc cgagatcgga ctcgaggcgg
     3301 cgcgcgatgg ctcgcgcgcc ctcgaatccg tgtgccgcct tcgccacctg ggcgcgtgag
     3361 ctcaacatca ccaggttgcc tgccgcgtag cccgcgtcgc tgcgcaccct gtcgatcgaa
     3421 gcgtcgcggc gctcgcctgc ggggccggac agcgcctcgc gggtgatcgg gcagtgcccg
     3481 acatcaagcc gctgcaggta gttcggcgtc acctggaaca actcgacact gcgcccgcgc
     3541 agccaggcgt gcagcctcag ttgaagccac tggcgaacgg tggcagtcgg cttcagcgtc
     3601 cttgggccga aggcggcctc gcctgccagc cagccatgtc gaagtggcga gggctcctgc
     3661 gcatacggag cgggcggtgt cacaccgtaa tgtgcatggt cccagcccag ctcgaagccg
     3721 gtgtgctcgt gcgtggtgag cagggcagca ggagcacggg tctcggcgca gggatggacc
     3781 acggctgaga ggtcgacggg aaacagatcg tttgcaaaca ggtcggtgtt catggcggac
     3841 tcctacggga cgcagcgaca tgccgcgatg gttgaagtct gtccaggcac aagctcatga
     3901 cgtgagccag cgaccgctga tgatcaggaa tttcgcctat gcttcgattg acggtggctc
     3961 atgtggtgag cgactcacgc gttaacggaa acagtcaggg gaggcccatg gatcggaccg
     4021 aacgcttcta caagatcgag ttgctgctgc gcagtcgtgg ctgcgtcagc ttcgacgcgc
     4081 tgctcgccga actggcggtg tcacctgcga cgctgaagcg cgacctgcag tacctgcgcg
     4141 accgcatggg cgctccgatc acctatgacc gcgacgacaa cggctaccgc ttcgatctgc
     4201 ccacgacagg tgcagcgaac tcttccaggg ccatgcacca cgagctgccg gggatgtggt
     4261 tcagcgagcg cgaactgcat gcactgctga cgatgcacca gctgatggcc ggcctcgatg
     4321 acgacggcgt gctcgctcgg cacctgcagc cgatgatgga ccggctgcag ggcctgctgg
     4381 gcgccgatgc ttcggaggcg agagagctgg tgcggcgcgt gaaggtgatc ggcaccgcac
     4441 gccgtcgtgt gccgagccgg cacttcgagt tgctgggcag cgcgctggtg cagcgcaagc
     4501 gggtgtggtt gcgctacttc aagcgcagcg atcgcgtgac cagcgagcga gaggtctccc
     4561 cacagcggct ggtgaactac cgcaacacct ggtacctgga cgcctggtgc cacgccagcc
     4621 agggcctgcg tcgctttgcg ctcgacgcga tcgaggcagc acgctcactg gacacccgag
     4681 ccagaccggt tgcactcaag gacctcgagg ccgaactcga tgccggctac ggcatctacg
     4741 gaggaggctc gaaggtgaaa tgggcgacgc tcgttttcga gcgggacgcg gcacagtggg
     4801 tcgcccacga ggagtggcac cccgaacaga aatcgcgctg gctgggcggg cgcagcggtg
     4861 agggcagcta tgaacttgtg gtgccgtaca gcgatcccac cgagttgatg atggacatcc
     4921 tgcgccacgg agacagcgtg cgcgtggccg gcgacaaggc gctggctgct gccgtggccc
     4981 agcgcctggc gaaagcggcg gcgctgtacg cttgatgcca tgcacttggc gactcgaagg
     5041 cagtcgcgac gcgaatccca cgaaaggaac cctgatgaaa gccacctgga acggcgtgac
     5101 cctggccgag agcgacgaca ccgtggtcgt cga
//
LOCUS       DAOORV010000229         5130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19538_length_5130_cov_7.316847, whole genome shotgun sequence.
ACCESSION   DAOORV010000229 DAOORV010000000
VERSION     DAOORV010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19538_length_5130_cov_7.316847"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKV56_17855"
     CDS             <1..209
                     /locus_tag="PKV56_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOX69717.1"
                     /translation="TGLIVTKLDGTAKGGVLAAIALWARQRGRGAGETGTTGPVPVYF
                     IGVGEKLEDLETFDAREFATALLG"
     gene            complement(228..1601)
                     /gene="ffh"
                     /locus_tag="PKV56_17860"
     CDS             complement(228..1601)
                     /gene="ffh"
                     /locus_tag="PKV56_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOX69718.1"
                     /translation="MASTLTERLSRAVKTMRGQARITESNVQEMLREVRMALLEADVA
                     LPVVRDFIARVKDKALGAEVVGSLSPGQALVGIVNRELAATMGSNEIGGIADINLTVQ
                     PPAVILMAGLQGAGKTTTTAKLAKHLIEKRKKKVLTVSADVYRPAAIEQLKTVTKQAG
                     AEWFPSSAADKPIDIANAALDYARKHYFDVLLVDTAGRLAIDEALMQEISALHAALNP
                     IETLFVVDAMQGQDAINTAKAFKEALPLTGIVLTKMDGDSRGGAALSVRQITGAPIKF
                     AGVSEKIDGLEVFDADRHAGRVLGMGDIVALVEQVTQGVDIAAAQKLADKVKSGDAFD
                     LNDFLQQISQMKKMGGLAGLMDKLPSQMTAKAGAVDMDRAERDVRRMEGMICSMTPLE
                     RRKPDLIKATRKRRIAAGAGVQVQEVNRMLNQFEQMRDMMKKMKGGGMMKMMKRLGGM
                     KGMGGMR"
     gene            1679..2521
                     /gene="ccsA"
                     /locus_tag="PKV56_17865"
     CDS             1679..2521
                     /gene="ccsA"
                     /locus_tag="PKV56_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOX69719.1"
                     /translation="MNAAAFPALLTAVSLMALLSYAVAAAVAERAPGGLRWFLLAGWL
                     LHAAAIGIDTTGIGTQVQGARFGFAPALSVTLWLVISVYVAESRSVPISGVRRALALL
                     GAIAVLLALMFPGELRPHAASRWAPIHWVLGIASYGLFGAAVLHAAMLNRADRQMRLN
                     LQPARLVGAGAPSSAAPPTGLPLMRLERLTFRFVSAGFVVLSMAIFLGWLFASPWRWD
                     HKVVFSILGWLVYAGLLAGRRRFGWRGPQATRWVYTGAVLLLLAYVGSRFVMEVLLNR
                     PLGV"
     gene            2562..2825
                     /locus_tag="PKV56_17870"
     CDS             2562..2825
                     /locus_tag="PKV56_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HOX69720.1"
                     /translation="MKFFLLLGLFLLVVWLFRGGRRSDAPPADDHSATTDPRDARPSG
                     HEEMVSCVHCGVHLPMSDAVGDAAGWFCGQAHRIAHENAVPPN"
     gene            2825..4729
                     /locus_tag="PKV56_17875"
     CDS             2825..4729
                     /locus_tag="PKV56_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69721.1"
                     /translation="MDIPAAPPSDRRKADRRKRPRGTRSGESWFGAFSGDADSQFHEE
                     GRREQAREADGAPADSGAEIDSRFLPRDGELRAPAGQSAFDRIYRAFLSARAALGVAL
                     IATVAAGGFFGALPGRGVMWVSLAYALIAVGMWLTPASGSEEEHATAPRLSRLQWLAG
                     IGVDIVCFTALHVLSKASSLNFAALLVLPVLMAGVLTRRVQALATAAAVALLLLGNAW
                     IGVLGGGDAASLMTQAGLAGSGLFVITVLAGELASRLAREELTAKDSMELARQQAQLN
                     RLVIEEMQDGVLVVDRTGRVRAANPAARRLLVRRGLGRPAPFQLRGVPAWDQLVKTVE
                     RGFHEAVWPEAGRDVVLQFEQGTTRTLRVRIRFTRRADEPSAEELCVLLLEDVRSMQA
                     RMRQEKLAAMGRVSAGIAHEIRNPLAAIAQANALLSEDASTPGQQQLTRMVADNVKRL
                     KRIVDDVMEVAPGTLSEATVIDASSEVAAACSDWAQAGSLDQGEGSVLKVDLPAEPLG
                     VVFDVEHLRRVLINLLDNARRHATDRPGSVHVRLSALDEATAQLSVCSDGPTIPIDVE
                     RYLFEPFFSTRSRGTGLGLYICRQLCEQYGASIDYRMRPAAEPNRNDFFVNMRRRAGL
                     AAEPRQQPPV"
     gene            4759..>5130
                     /locus_tag="PKV56_17880"
     CDS             4759..>5130
                     /locus_tag="PKV56_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69722.1"
                     /translation="MSSPAYFSLLVVDDEPDLRTLYELTLLREGYDIETAGSVEEARL
                     HLQDRVYSAVITDMRLPDGTGLDLLSYLESNARREKAIVITAYGSAENAVEALKAGAY
                     DYLTKPVDLKQFRAVVASALGR"
BASE COUNT          743 a         1660 c         1758 g          969 t
ORIGIN      
        1 tcaccggcct gatcgtcacc aagctcgacg gcacggccaa gggcggcgtg ctggccgcaa
       61 tcgcactgtg ggctcgacag cgtgggcgcg gggccggaga aaccggcaca accggcccgg
      121 ttccggtcta tttcatcggc gtcggcgaga agctcgagga cctggaaacc ttcgatgcaa
      181 gggagttcgc aaccgcgctg ctgggctgat cgtgcgctgg ccccagctca gcgcatgccg
      241 cccattcctt tcatgccgcc gaggcgcttc atcatcttca tcatgccgcc gcccttcatc
      301 tttttcatca tgtcgcgcat ctgctcgaac tgattgagca tgcggttgac ttcctgcacc
      361 tgaacgccgg cgcctgctgc gatgcggcgc ttgcgggttg ccttgatcag atctggcttg
      421 cgtcgttcga gcggggtcat cgagcagatc atgccctcca tgcggcgcac gtcgcgctcg
      481 gcgcgatcca tgtcgaccgc acccgccttg gccgtcatct gggacggcag cttgtccatc
      541 agaccggcga gtccgcccat cttcttcatc tgcgagatct gctgcaggaa gtcgttcagg
      601 tcgaaggcat cgcctgattt gaccttgtcg gcgagcttct gcgccgcggc gatgtcgacg
      661 ccctgcgtga cctgctcgac cagcgcgacg atgtcgccca tgccgagcac gcggcctgcg
      721 tggcgatctg catcgaacac ctccagtccg tcgatcttct cgctgacccc tgcgaacttg
      781 atcggcgcgc ccgtgatctg tcgcaccgac agcgccgcgc cgccacgcga atcgccgtcc
      841 atcttggtca gcacgatgcc ggtgagaggc agcgcttcct tgaaggcctt cgcggtgttg
      901 atcgcgtcct ggccctgcat ggcgtcaacc acgaaaagcg tttcgatcgg gttgagcgca
      961 gcatgcagcg cgctgatttc ctgcatcaag gcctcgtcga tggcgagccg accggcggtg
     1021 tctaccagca gcacgtcgaa gtaatgcttg cgggcgtagt cgagggcggc gttcgcgatg
     1081 tcgatcggtt tgtccgcggc gctcgacggg aaccattcag cgcctgcctg cttcgtcacc
     1141 gtcttcagct gctcgatggc ggccggacgg tacacgtcgg ccgagaccgt cagaaccttt
     1201 ttcttgcgtt tctcgatcag gtgcttggcg agcttcgcgg tcgtcgtcgt cttgccggcg
     1261 ccctgcaggc cggccatcag gatcaccgcc ggaggctgca ccgtcaggtt gatgtcggca
     1321 atcccgccaa tctcgttgct gcccatcgtc gccgcgagct cgcggttgac gatcccgacc
     1381 aatgcctgac ctggtgacag cgagcccacc acctcggcgc ccaacgcctt gtccttcacc
     1441 ctggcgatga agtcgcgcac caccggcagc gccacgtcgg cttccagcag cgccatgcgg
     1501 acctcgcgca gcatttcctg cacgttggat tcggtgatgc gggcctggcc gcgcatcgtc
     1561 ttgacggcgc gactcagtcg ctctgtgagg gtggaggcca tggggaggtc ggcggcgccg
     1621 ggagcggcgt gacgcgaaag tacactttgg cgatgagtct atcttctgag gtccgtcggt
     1681 gaatgcggct gcgttcccgg ccttgttgac tgccgtcagc ctgatggccc tgctgagcta
     1741 cgctgttgct gcagcggtgg cggaacgcgc gcccggcgga ttgcgctggt ttctgctggc
     1801 cggctggctg ctgcatgcag cggcgatcgg tatcgacacc accggcatcg ggacgcaggt
     1861 gcagggtgcg cgcttcggct tcgcgccggc tctttcggtg acgctgtggc tggtgatcag
     1921 cgtctacgtc gcggagagca ggtccgtgcc gatcagtggt gtgcggcgcg cgctggcgct
     1981 cctgggcgcg attgcagtgt tgctggcgtt gatgtttccg ggcgaactcc ggccgcacgc
     2041 cgcgtcacga tgggcgccca tccactgggt gctcgggatc gcgtcttacg gcctgttcgg
     2101 tgccgccgtg ctgcatgccg cgatgctcaa ccgggccgat cggcagatgc gactgaacct
     2161 gcagccggcc cggctggtgg gggcgggcgc gccgagctcc gcagcaccgc caacgggcct
     2221 gccgctgatg cgtctggagc gcctcacgtt ccgcttcgtg tctgcgggtt ttgtcgtgct
     2281 ttcgatggcg atcttcctcg gctggctgtt cgcgagtccc tggcgatggg atcacaaggt
     2341 ggtgttctcg atccttggct ggctcgtgta tgccgggctg ctggcaggcc gccgccgttt
     2401 cgggtggcgc ggaccgcagg ccacccgctg ggtatacacc ggtgcggtcc tgctgctgct
     2461 ggcatacgtg ggttcgcgct tcgtgatgga ggttctgttg aaccggcctc tgggcgtgtg
     2521 atcgaagcag gcccagggtg acttagaggg cacgagccgc gatgaaattc ttcctgctgc
     2581 tgggtttgtt cctgctggtg gtgtggctgt tccgcggagg gcgacgcagt gatgccccgc
     2641 ctgcggacga ccactctgca accaccgacc cgcgggacgc acgcccgtcc gggcacgagg
     2701 aaatggtttc ctgcgtccat tgcggtgtcc atctcccgat gagcgacgca gttggtgatg
     2761 ctgcgggctg gttctgcggg caggcgcacc gcatcgctca cgagaacgcc gtgccgccga
     2821 attgatggac ataccggccg cgccaccgag cgaccgccgc aaggcagatc gtcgcaaacg
     2881 accgcgcggc acacgctccg gtgaatcgtg gtttggcgcc ttcagcgggg atgcggattc
     2941 gcagtttcac gaggaggggc ggcgggagca agcgcgcgaa gctgatgggg ctcctgcgga
     3001 ctcgggggcc gagatcgact cccggtttct gccgcgggat ggtgaactca gggcgcccgc
     3061 cgggcaatcc gcgttcgatc ggatctaccg tgctttcctg agcgcccgcg ccgcactcgg
     3121 cgtggcgttg atcgccaccg tggcggcggg cggcttcttc ggggcgctgc ctggccgcgg
     3181 cgtgatgtgg gtcagcctcg cctacgccct gattgcggtc ggcatgtggc tgaccccggc
     3241 ttccgggtcc gaggaggagc acgcaacggc gccacgtctg agccgcctcc agtggctggc
     3301 gggaatcggt gtcgacatcg tgtgcttcac ggcactgcat gtgctgtcga aggccagcag
     3361 cctcaacttt gccgcgctgc tggtgctgcc ggttctcatg gcgggcgtgc tgacgcggcg
     3421 tgtgcaggcg ctggcgaccg cggcggcagt cgcactgctg ctgctcggca atgcctggat
     3481 cggtgtgctg ggcggcggcg atgcggcgtc cctgatgacg caggccgggc tggctggcag
     3541 cgggctgttc gtcatcaccg tgctggcggg cgaactcgcc agccggttgg cgcgcgagga
     3601 gctcacggcc aaggacagca tggaactggc gcgtcagcag gcccagctca accgcctggt
     3661 gatcgaggaa atgcaggacg gtgtcctggt ggtcgaccga accggccggg tgcgcgcggc
     3721 caatccggct gccaggcgct tgctggtgcg gcgcggcctc ggtcggccgg cgccgttcca
     3781 actgcgcggc gttccggcgt gggatcaact cgtcaagacg gtcgaacgcg ggtttcatga
     3841 agcggtctgg cccgaagccg gccgtgacgt cgtgctgcag ttcgagcagg gcacgacccg
     3901 caccttgcgt gtgcgcatcc gtttcactcg acgcgccgac gagccatcgg ccgaggaact
     3961 gtgcgtgctg ctgctcgagg acgtgcgcag catgcaggcc cggatgcggc aggagaaact
     4021 cgcggccatg ggtcgggtgt ctgctggaat cgcgcacgaa atccgcaatc cgctggctgc
     4081 gatcgcgcag gccaacgctc tgctttcgga ggatgcttcc acgccgggcc agcagcagct
     4141 gacgcggatg gtggccgaca acgtcaagcg actcaagcgc atcgtggacg acgtgatgga
     4201 ggtggctccg ggtaccttga gcgaggccac cgtgatcgac gccagttccg aggtggcggc
     4261 cgcttgcagc gactgggccc aggcaggctc gctggatcag ggcgagggca gcgtgctcaa
     4321 ggtcgatctc cccgcggaac cgctcggcgt ggtctttgat gtcgagcatc tgcggcgcgt
     4381 gctgatcaac ctgctcgaca acgcccgccg ccacgccacc gaccgcccgg gctcggtgca
     4441 cgtgcgtctg agcgcgctcg acgaagccac ggcacaactc agtgtctgca gcgacggccc
     4501 gacgatcccc atcgatgtcg agcgctatct gttcgagccg ttcttctcga cgcgcagccg
     4561 gggcactggg ttgggcctgt atatttgccg gcagctctgc gagcagtacg gagccagcat
     4621 cgactaccgg atgcgtccgg cagccgagcc gaaccgcaat gatttcttcg tcaacatgcg
     4681 tcgccgggcc gggctcgccg ccgagccgcg gcagcagcct ccggtgtgat gcacggaagc
     4741 attgaacgag tcttcgacat gtcttccccc gcttacttca gcctgctggt cgtcgatgac
     4801 gagcctgacc tgcgcacgct ctacgaactg acgctgctgc gagagggcta cgacatcgag
     4861 accgccggca gtgtcgagga agcccggctg cacctgcagg accgtgtcta cagcgccgtc
     4921 atcaccgaca tgcggctgcc cgatggcacg gggctcgacc tgctgagcta cctggaaagc
     4981 aatgcccgcc gagagaaggc catcgtcatc accgcctacg gatcggcaga gaacgcggtg
     5041 gaggcgctga aggctggcgc ctacgattac ctgaccaagc cggtcgacct gaagcagttt
     5101 cgtgcggtgg tcgcgtcagc tctcggcagg
//
LOCUS       DAOORV010000230         5089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19730_length_5089_cov_5.607469, whole genome shotgun sequence.
ACCESSION   DAOORV010000230 DAOORV010000000
VERSION     DAOORV010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19730_length_5089_cov_5.607469"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="PKV56_17885"
     CDS             <1..2623
                     /locus_tag="PKV56_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOX69723.1"
                     /translation="SVEGIDEYRLANGLQILLIADDSKPTTTVNLTYHVGSRHENYGE
                     TGMAHLLEHLMFKGSPKHPNVWSEFTKRGLAANGSTWFDRTNYTASFSANDDNLRWYL
                     GWQADAMTHSFIARKDLDTEMTVVRNEMESGENNPDRILFSKTLATMYQWHNYGKDTI
                     GSRSDVENVDIPRLQAFYRQYYQPDNATLIVSGKFQSAKVLQWIAQAFGVIKKPTRVL
                     PALYTLDPTQDGERSVTLRRSGGVPVLYAAYHIPPGSHPDYAAVELLGMLMGEAPAGR
                     LHKRLTEKGLASTTYAFAQGLAEPGFALFGAQLGPNQDVDAARAELLATLESVSREPI
                     TTEELERARIKWLKSWEQAFTDPETIGISLSESVAQGDWRLFFLTRDRIRAATLADVQ
                     RVAEQRLLPANRTLGVYLPTDQPQRSPAPARVDIAAELKSLKPKGAEAAVESFEATPA
                     NIDRRTQRSEIGGLRVALLPKGTRGASVQATLNLHFGDEKSLFGQGEVAETVVALLDK
                     GTATMTRQQVQDRLDALHTEIRIGGGAGSVSVQLSSRREHLAGAIALVADLLRHPSFP
                     AESLLEVKRQSLSSIEQARKEPHAVISNALARLGNPYPRGDVRYARTFDEIVEDVNGV
                     TLEQVREHHRRFYGASRAEFGAVGDMDVAAVRQALQTAFGDWKSGEPFARIPQPIVAV
                     PAQRLMLKTPDKQNATMRVELPVPLSDNDADYAALTMANYLLGSGGSSRLWKRIREVE
                     GLSYDVRSTMDWNNIEQHSEWSASAIFAPQNRPKVEAAFREEIARALKDGFTAKELAE
                     GQRGLLNFRRLSRAQDASVAAGLAGNLYLGRTFARSAEVDAQLDALTLSQVNDALRRY
                     LKPENFVYGFAGDFKQP"
     gene            complement(2610..3797)
                     /locus_tag="PKV56_17890"
     CDS             complement(2610..3797)
                     /locus_tag="PKV56_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX69724.1"
                     /translation="MFRVDTQGKTASKNYVIRVHDQPAGIAAAAWNALLGEQPSATPF
                     MRHEYLTALHESGSATAETGWRPQFLSVCEGDTLVAACPLYIKDHSYGEYVFDWAWAD
                     AYRRHGLDYYPKLLAAVPFTPVPGPRLLARTADSRLALLSGMQQLATQAGLSSAHLLF
                     LDEADSVAAREAGWMMRTTVQFHWTNREPTPYADFGDFLSSLQREKRKKIQQERRRVT
                     DAGVSFDALRAGEISDDDWDFFYRCYTLTYQAHRSTPYLTRDFFHRMALTMPECWLMF
                     VARRDGARIASSLIGIDTVRGAAFGRYWGATEPVNCLHFEACYYQPLAWCIANGFRRF
                     EGGAQGEHKMARGLLPVQTESAHWLAHPQFAQAVADFLKREGAGVDQYVDELNDRRPF
                     KAA"
     gene            3796..>5089
                     /locus_tag="PKV56_17895"
     CDS             3796..>5089
                     /locus_tag="PKV56_17895"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOX69725.1"
                     /translation="MPSNPAVKVALAQINATVGDLTGNAAKIVEFSRRAHAQGARLVV
                     TPELSLCGYPPEDLLLRPAFMHACREALAECAAALADLPGLHVVVGHPHQWLGQSDVR
                     TKSHTVPQRHNAASVLAEGRVIATYCKRELPNYQVFDERRYFASGRDAGREPVVFEVA
                     GLKFGLAICEDAWFDEPARSARAAGAQVLCVLNASPYHLGKVDERESRMGDRARDVGM
                     PILYAHLTGGQDEVVFDGASFAVDATGHVRARAPMFAESLLMLDVSDGEVAGSVTEVP
                     CIESQAWSALVTGVHDYIGKNGFPGALIGLSGGVDSALVLAIAVDALGPDRVRAVMMP
                     SAYTADISWIDARAMARGLGVRYDEISIEPMFSSFKESLASEFAGLPADATEENIQAR
                     IRGTLLMALSNKFGSIVLTTGNKSEMATGYCTLYGDMAG"
BASE COUNT          928 a         1617 c         1661 g          883 t
ORIGIN      
        1 gtccgtggag ggcatcgacg aataccggct cgccaacggt ctgcagatcc tgctgatcgc
       61 cgacgactcg aagccgacaa ccaccgtcaa tctgacctat cacgtcgggt cacgacatga
      121 gaactacggt gagaccggca tggctcacct gctggagcac ctgatgttca aggggtcgcc
      181 gaagcatccc aacgtctggt ccgaattcac caagcgaggt ctggcggcca atggcagcac
      241 ctggttcgac cgcaccaact acaccgccag tttctcggcc aatgacgaca acctgcgctg
      301 gtatctcgga tggcaagccg atgcgatgac ccacagcttc atcgcgcgca aggaccttga
      361 caccgagatg acggtggtgc gcaacgagat ggagtcgggc gagaacaacc ccgatcgcat
      421 cctgttttct aagacgctgg cgacgatgta ccagtggcac aactatggca aggacacgat
      481 cggctcgcgt tcggacgtcg agaatgtcga catccctcgc ctgcaggcgt tctaccgcca
      541 gtactaccag cccgacaacg cgacgctgat cgtgtcgggc aagttccagt cggccaaggt
      601 cctgcagtgg atcgcccagg ccttcggtgt cataaagaaa ccgacccgag tgctgcccgc
      661 gctttacacg ctggacccca cgcaggatgg tgagcgcagc gtcacgctgc gtcgcagcgg
      721 cggcgtgccc gtgctctatg cggcctacca cataccgccc ggctcgcacc cggactatgc
      781 cgcggtcgag cttctgggca tgctgatggg cgaagccccg gccggcagac tgcacaagcg
      841 attgaccgaa aagggtctgg catcgaccac ctatgcgttc gcgcaaggtc tggccgagcc
      901 gggcttcgca ctcttcggcg cgcagctggg accaaaccag gatgtcgatg ccgcccgagc
      961 cgagttgctc gccacgctgg agtcggtctc ccgggagccg atcaccaccg aggagttgga
     1021 gcgggcccga atcaagtggc tgaagagctg ggaacaggca ttcaccgacc ccgaaaccat
     1081 cggcatctcg ctgagcgaaa gcgtggcgca gggagattgg cggttgttct ttctgacgcg
     1141 cgatcgcatt cgcgctgcga cgctcgcgga cgttcagcgc gtggccgagc agcgcctgct
     1201 gccggccaat cgcacgctcg gcgtgtacct gccgactgat cagccacagc gctcgccagc
     1261 ccctgctcgg gtcgacattg cggctgaact gaaatcactc aagcccaagg gtgccgaagc
     1321 ggccgtcgaa tccttcgagg ccactccggc caacatcgat cgccgcaccc agcgttccga
     1381 gatcggcggg ctgcgtgtcg cgttactgcc caagggcacc cggggcgcct ccgtgcaagc
     1441 cacgctgaac ctgcacttcg gcgacgagaa gtcactgttc ggtcaggggg aagtggccga
     1501 gacggtggtg gccctgctcg acaagggcac agcaacgatg acgcggcagc aggtacagga
     1561 ccggctggat gcgctgcaca ccgagattcg catcgggggt ggtgccggct cggtatcggt
     1621 tcagctcagt tcgcggcgcg agcatctggc cggtgcgata gcactggtcg cagacctgtt
     1681 gcgtcatccg agctttccgg ccgagtcgct gctggaggtg aagcgacagt cgctgtcttc
     1741 gatcgagcag gcacgcaagg aaccccatgc cgtgatcagc aatgcattgg cgcggctggg
     1801 caacccctac ccgcgcggcg acgtgcgcta cgcaaggacc ttcgacgaga tcgtggagga
     1861 tgtcaacggc gtgacgctcg agcaggtacg cgagcaccac cggcgtttct acggcgcatc
     1921 ccgagccgag tttggtgcgg tcggcgacat ggacgtggcc gctgtgcggc aggcgctgca
     1981 aaccgcattc ggcgactgga aatccggaga accattcgca cgcatcccgc agcccatcgt
     2041 ggccgtgccg gcgcaacgtc ttatgctgaa gacgcccgac aagcagaacg cgacgatgcg
     2101 ggtcgaactg cctgtgccgc tgtcggacaa cgacgccgac tacgctgcac tgacgatggc
     2161 gaattacctg cttggctccg gcggtagttc gcgcctgtgg aagcgcatcc gcgaagtcga
     2221 gggcctgtcg tatgacgtgc gcagcacgat ggactggaac aacatcgagc agcattcgga
     2281 atggtcggcc agcgccatct tcgcgccaca gaaccggccc aaggtcgagg cggcattccg
     2341 cgaggaaatc gcacgcgcgc tgaaggacgg attcaccgcg aaggagctgg ccgaaggtca
     2401 gcgcggactg ttgaacttcc gccgcttgtc gcgcgcccag gatgcctccg tggccgccgg
     2461 actggcgggc aacctgtatc tcgggcgcac cttcgcgcga tccgccgaag tcgatgcgca
     2521 actggacgcg ctgaccctgt cgcaagtcaa cgatgcgctg cgccgctatc tgaagcccga
     2581 gaacttcgtc tacggcttcg ccggggactt caagcagcct tgaatggtcg gcggtcgttc
     2641 agttcgtcca catactggtc gactccggca ccttcgcgct tcaggaagtc ggccacagcc
     2701 tgcgcgaact gggggtgtgc cagccagtgc gcagactctg tctgcaccgg caggagtccg
     2761 cgcgccatct tgtgctcgcc ctgcgctccg ccttcgaaac gccggaaccc gttcgctatg
     2821 caccaggcca ggggctggta gtaacaggcc tcgaagtgaa gacagttcac cggctcggtc
     2881 gccccccagt agcgtccgaa ggcggcgccc cggacggtgt cgatgccgat caacgaggac
     2941 gcaatccggg cgccgtcgcg tcgggccacg aacatcaacc agcactcggg catcgtcagg
     3001 gccatgcggt ggaagaagtc gcgcgtgagg tagggcgtcg atcggtgcgc ctggtaagtc
     3061 agcgtgtagc accggtagaa gaagtcccag tcgtcatcgg aaatttcgcc tgcccgaagc
     3121 gcatcgaagc tcactccggc gtccgtgacg cgtcgccgct cctgctggat cttcttgcgt
     3181 ttctcgcgct gcaggctgga gaggaagtcg ccgaaatcgg catagggcgt cggttcccgg
     3241 ttggtccagt ggaattgcac cgtggtgcgc atcatccagc cggcctcgcg ggccgcgacg
     3301 ctgtcagctt catcgagaaa gagcaggtgg gccgacgaca gccccgcttg cgtggcgagt
     3361 tgctgcatgc cgctgagcaa tgccagccgt gaatccgcgg tgcgcgccag cagtcgcggt
     3421 cccggcactg gcgtgaacgg caccgcagcg agcagctttg ggtagtagtc caggccatgc
     3481 cggcgatagg catcggccca ggcccagtcg aaaacatact cgccgtagga atggtccttg
     3541 atatacagcg gacaggcggc caccagggtg tcgccctcgc ataccgacag gaattgcggc
     3601 cgccagcctg tctccgccgt ggcactgccg gactcatgca gcgcggtgag gtattcgtgc
     3661 cgcatgaaag gagtcgcgct cggttgctcg ccgagcagcg cgttccatgc cgccgcggca
     3721 atgccagccg gctggtcatg gacccggatg acataattct tggacgctgt tttcccctgc
     3781 gtatccaccc tgaacatgcc ttcaaatcct gcggtcaaag tcgccctggc gcaaatcaac
     3841 gcgaccgtgg gggacctgac cgggaacgcg gccaagatcg tcgaattttc tcggcgcgcg
     3901 cacgcacagg gggcccgctt ggtggtgact ccggagctga gtctgtgcgg ttacccgccc
     3961 gaggacctgc tgctgcgccc tgcattcatg catgcgtgcc gcgaggcgct cgctgagtgc
     4021 gccgcagcgc tggccgacct gccaggcttg catgtggtgg tgggtcatcc gcatcagtgg
     4081 ttagggcaat ccgatgttag gacgaagtcg cacaccgtgc ctcagcgcca caacgcagcc
     4141 tcggtattgg ccgagggacg ggtgatcgcg acctactgca agcgcgagtt gcccaactac
     4201 caggtcttcg acgaaaggcg ctatttcgca tcgggccgcg atgcgggccg ggagccggtc
     4261 gtgttcgaag tcgccggctt gaagttcggt ctggccatct gcgaggacgc ctggttcgat
     4321 gaacctgctc gctcggcaag agccgcaggt gcgcaggtac tgtgcgtact gaacgcctcg
     4381 ccctatcacc tgggcaaggt ggatgagcgt gaatcccgca tgggcgaccg tgcgcgcgat
     4441 gtcggcatgc ccatcctcta cgcccatctg accggtgggc aggacgaggt ggtcttcgac
     4501 ggcgcgtctt ttgctgtcga cgcgacaggc cacgtgaggg ctcgcgcgcc catgttcgct
     4561 gaatccctgc tgatgctgga cgtctcggat ggcgaggtgg cgggctcagt cacggaagta
     4621 ccgtgcatcg aatcgcaggc ctggtccgcc ctggtcactg gcgtgcatga ctacattggc
     4681 aagaacggtt tccctggtgc gctgatcggc ctgagcggcg gcgtcgattc ggccctggtg
     4741 ctggcgatag cggtcgatgc gctggggccc gaccgcgtgc gggcggtgat gatgccgtcg
     4801 gcctacaccg ccgacatctc gtggatcgac gcccgagcca tggccagagg attgggagtg
     4861 cgctacgacg agatctcgat cgagccgatg ttctcgtcgt tcaaggaaag ccttgcatcc
     4921 gagttcgcgg gcttgcccgc tgacgcgacc gaggagaaca tccaggcccg catccgtggc
     4981 acgttactga tggcgttgtc gaacaagttt ggctcgatcg tgctgaccac agggaacaag
     5041 agcgagatgg ccaccggcta ctgcacgctc tacggcgaca tggccggcg
//
LOCUS       DAOORV010000231         5076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_19800_length_5076_cov_8.534555, whole genome shotgun sequence.
ACCESSION   DAOORV010000231 DAOORV010000000
VERSION     DAOORV010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19800_length_5076_cov_8.534555"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKV56_17900"
     CDS             <1..339
                     /locus_tag="PKV56_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOX69726.1"
                     /translation="VDRTKDMLLCSGFNVYPRVLEEAIYQHPAVREVAVIGIDDEYRG
                     QSPKAFITLKPGAESFTLKDLQGFLKDKLGKHEMVQALEIRAELPKTAVGKLSKKDLY
                     DEEAKKKLTS"
     gene            388..1719
                     /locus_tag="PKV56_17905"
     CDS             388..1719
                     /locus_tag="PKV56_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69727.1"
                     /translation="MNSTATATRGGPIPGGHNADHVEQVYSKVGWRIMPLLFVAYAIA
                     YLDRINIGYAQLQMKQTLPWEATVYGLGAGIFFVGYFLFEVPSNLMLEKIGARKTLLR
                     IMFCWGIVAAAMAFVTTPTQFYIARFLLGAFEAGFFPGIILYLTFWYPSARRGQMIAI
                     FLSATTIMSILAGPLSGATLKYMNGLYGLHGWQWLFIVQGLPASMLGIACYLYLQDKP
                     EHAKWLSANEKKVLRDHLDHDHAPVDGASHGSFMQSLRDPKVYVLSLAYFFAIGGTYA
                     LVFWLPTLVRSWGVADLFMIGIYTAIPSVFGAIGMILFGRSSDRHSERRWHYVAAAFL
                     AAAGLALTAMMQGNLVGSLIGMSIASIGISSLTPMLFTITSEYLSKAAAAGGIALISS
                     LGNLGSAASPVAAGALTTSTGSPIATLYMVLVMFILAGLIVMFAVRPHKGP"
     gene            1813..2214
                     /locus_tag="PKV56_17910"
     CDS             1813..2214
                     /locus_tag="PKV56_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69728.1"
                     /translation="MSFVHCWEPSGITTTDRGDLVLEEVLESHQRMAADPRFDDLKFM
                     IVDTLPVERVALIESDVVQINAFLTGPALTNPNIFAVVIALHPAVLRFVAVYAQLPDR
                     RYDMRVCSSMVSARQLLAHRDLRAIHGKGKA"
     gene            2216..2770
                     /gene="ppa"
                     /locus_tag="PKV56_17915"
     CDS             2216..2770
                     /gene="ppa"
                     /locus_tag="PKV56_17915"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOX69729.1"
                     /translation="MKLESLPVGSDPPHEVNAVIEVPIGGEPIKYEMHKAAGMLVVDR
                     FLYTPMRYPGNYGFIPHTLSEDGDPCDVLVANTRPLMPGSAIAVRPVGVLRMTDEAGG
                     DEKIVAVPATRLTQRYAHVHNYTDLPEIAWRQIEHFFAHYKDLEPGKWARFEGWGDAD
                     LAKELIERAIARAAKIKGVAETLR"
     gene            2767..3423
                     /gene="adk"
                     /locus_tag="PKV56_17920"
     CDS             2767..3423
                     /gene="adk"
                     /locus_tag="PKV56_17920"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOX69730.1"
                     /translation="MRLILLGPPGAGKGTQADRIAQHFGIPQLSTGNMLRAAAKAGTP
                     VGLAAKRVMDRGALVGDDIIVCLVVERIAQPDCAAGFLLDGFPRTLPQVEAMKDVRIK
                     IDCVLEIDVPEEEIVRRMSGRRVHAASGRSYHVVFNPPTVAGKDDVTGEDLVQRDDDA
                     EETVRKRLAVYRDQTRPLLAHYAAWEATRDPNAPRCRRINGMGGVDAVAALALAAVAG
                     "
     gene            3437..4054
                     /locus_tag="PKV56_17925"
     CDS             3437..4054
                     /locus_tag="PKV56_17925"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX69731.1"
                     /translation="MSSITRELNPDERDRLLAHLLALSPEDRRLRFAHAIADDGLQRY
                     VEGIDFTRDTVFVATDAELHVVGAAHVARDGEHAELGVSVLPQARGHGIGAALLERSA
                     IHARNAGVRVLFMNCLVENAPMLHLARKQGLQVAVSSGEAEAFVRLPRPSLRSLASEA
                     VADHLGLIDHAHKAHWVALRGLWLASFGGESEPPRGGSETQEGSS"
     gene            4056..4679
                     /locus_tag="PKV56_17930"
     CDS             4056..4679
                     /locus_tag="PKV56_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX69732.1"
                     /translation="MVTTSKKSKRTAPQAVGELPLLSLARQVRSWTDTLLGVAGSAGD
                     IGMTLAQARIRDPEKKAAVGKAGSQLRQWREEAGLTAKELSDAVGLGDTKLIEQAEGG
                     TAALPFEIVLRLAGVLGRRDPIPVAMGLTRQYNPELWKALESIGIGRLAVQGTREREL
                     ANVYRGNDAARALDDEAFAQVLAFTKQAFDMAVDFSRRPARAKTKPG"
     gene            4725..4857
                     /locus_tag="PKV56_17935"
                     /pseudo
     CDS             4725..4857
                     /locus_tag="PKV56_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484026.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
BASE COUNT          887 a         1660 c         1676 g          853 t
ORIGIN      
        1 gtcgaccgca ccaaggacat gctgctgtgc agcggcttca acgtctaccc gcgcgtgctc
       61 gaagaggcga tctaccagca tccggcggtg cgcgaggtgg cggtgatcgg catcgacgac
      121 gagtaccgcg gccagtcgcc gaaggccttc attaccttga agcccggcgc cgagtcgttc
      181 acgctgaagg acctgcaggg cttcctgaaa gacaagctcg gcaagcacga gatggtgcag
      241 gcattggaga ttcgagccga gctgccgaag acggccgtcg gcaagttgtc caagaaggac
      301 ctgtacgacg aagaagcgaa gaagaagctg acttcctgac aagtgacggc agtcggccga
      361 gttcacacca caagaccgga gacaaccatg aactcaactg caaccgcgac acgcggtggc
      421 cccatccctg gcggacacaa cgccgaccat gtcgagcagg tctattccaa ggtcggctgg
      481 cgcatcatgc cgctgctgtt cgttgcctac gcgatcgcct acctcgaccg catcaacatc
      541 ggctacgcgc agctgcagat gaagcagacg ctgccctggg aagcaaccgt ctacggtctg
      601 ggcgccggca tcttcttcgt cggctacttc ctgttcgaag tgccgagcaa cctgatgctg
      661 gaaaagatcg gcgcacgcaa gacgctgctg cgcatcatgt tttgctgggg catcgtcgcc
      721 gcggcgatgg ccttcgtgac gacgccgacc cagttctaca tcgcacgctt cctgctcggt
      781 gcgttcgagg cgggcttctt ccccggcatc atcctgtacc tcaccttctg gtacccgtcg
      841 gcgcgccgcg gccagatgat cgccatcttc ctgagtgcga cgaccatcat gtcgatcctc
      901 gccgggccct tgtccggcgc gacgctgaag tacatgaacg gcctctacgg gctgcacggc
      961 tggcaatggc tgttcatcgt gcaggggctg ccggcgtcta tgctgggcat cgcttgctac
     1021 ctgtacctgc aggacaaacc cgaacatgcc aagtggctgt ccgcgaacga gaagaaggtc
     1081 ctgcgcgacc acctcgatca tgaccacgct cccgtcgacg gtgcctcgca cggttcgttc
     1141 atgcagtcct tgcgcgatcc gaaggtgtat gtgctgtcgc tggcgtactt ctttgccatc
     1201 ggcggcacct acgcgcttgt gttctggctg ccgaccctgg tcaggagctg gggtgtcgcc
     1261 gacctcttca tgatcggcat ctacacggcc attccgagcg tgttcggcgc catcggcatg
     1321 atcctgttcg gccgcagttc cgaccgccac agcgaacggc gttggcacta cgtggccgca
     1381 gcgttcttgg ccgccgccgg cctggccctc acggcgatga tgcagggcaa tctggtcggc
     1441 tcgctgatcg gcatgtcgat cgccagcatc ggcatctcgt cgctgacgcc gatgctcttc
     1501 acgatcacct ccgagtacct gtccaaggca gccgccgcgg gcggcatcgc gctcatcagc
     1561 agcctgggca acctcggctc ggccgcgagc ccggtcgcgg ccggcgcgct gaccacgagc
     1621 accggcagcc cgatcgccac cttgtacatg gtgctggtca tgttcatcct cgccggcctg
     1681 atcgtgatgt tcgcggtgcg gccgcacaag gggccgtgag cctgctttcg aggatgcgat
     1741 gccgggcgat ggtcagatgc ccggcaccgt tgtcgcctgc ttcgctgggc aacggcaatt
     1801 caaacttcca gcatgtcatt cgtccactgt tgggaaccat cagggatcac caccaccgac
     1861 cgcggcgatc tcgtgctgga agaggttctg gagtcgcacc agagaatggc agcggatccg
     1921 aggttcgacg acctgaagtt catgatcgtc gatacgctgc ctgtcgaaag ggttgcattg
     1981 atcgagtccg acgtggtgca gatcaacgcg ttcctcaccg ggcccgccct gaccaacccc
     2041 aacatcttcg ccgtcgtcat tgccttgcat cccgcggtct tgcgttttgt cgctgtgtac
     2101 gcgcaattgc cggatcgaag gtacgacatg cgagtctgct cgtcgatggt atcggcgcga
     2161 caactgctcg cgcatcggga cctgcgggca atccacggca agggaaaggc gtgagatgaa
     2221 actggagagc cttccggtcg ggagcgatcc cccgcacgaa gtgaatgcgg tcatcgaggt
     2281 gccgatcggt ggcgaaccca ttaagtacga gatgcacaag gcagcgggca tgcttgtcgt
     2341 cgaccggttc ctctacacgc cgatgcgcta cccgggcaac tacggcttca ttccgcacac
     2401 cctgtccgag gacggcgacc cctgtgacgt gctggtggcc aacacgcgcc cgctgatgcc
     2461 gggctcggcc atcgccgtac gtcccgtggg cgtgctcagg atgacggacg aagccggcgg
     2521 cgacgagaag atcgtggcgg taccggccac acggctgacg cagcgttacg cccacgttca
     2581 caactacacc gacctgcccg agatcgcgtg gcgccagatc gagcacttct tcgcgcacta
     2641 caaggacctc gagccgggca agtgggccag gttcgagggc tggggcgacg ccgatctagc
     2701 caaagagctg atcgagcggg ccattgcgcg cgccgcgaag atcaagggcg tcgcggagac
     2761 gctgcgatga gactcatcct gttggggccg ccgggcgcgg ggaagggcac gcaagccgat
     2821 cgcatcgcgc agcacttcgg catcccgcag ttgtctacgg gcaacatgct gcgggctgcg
     2881 gccaaggcag ggacgccggt cggcctggcc gccaaacgcg tgatggaccg gggcgcactc
     2941 gtgggcgacg acatcatcgt ctgtcttgtt gtggagcgca tcgcccagcc cgactgcgcc
     3001 gcaggcttcc tcctagacgg ctttccgcgc accctgccgc aggtcgaagc catgaaggat
     3061 gtgcgcatca agatcgactg cgttctcgag atcgatgtgc ccgaagaaga gatcgtccgg
     3121 cgcatgagcg gccggcgcgt gcacgcggca tcgggccgca gctaccacgt cgtgttcaac
     3181 ccgccgacgg ttgccggcaa ggacgacgtg accggcgagg acctcgttca gcgcgatgac
     3241 gacgcggaag agaccgtgcg caagcgcctg gctgtctacc gggaccagac gcgcccgctg
     3301 ctcgcccact acgcggcgtg ggaggccacg cgcgacccga acgcgccgcg ttgccgccgc
     3361 atcaacggca tgggcggtgt cgacgcggtc gcggcgctcg cgctcgcggc agtcgcaggc
     3421 tgacccgcag gcttcaatga gctcgatcac ccgcgagctg aatccggacg agcgcgaccg
     3481 cctgctcgcc catctactcg cactgtcacc cgaggatcgg cgcctgcgct ttgcccatgc
     3541 aatcgccgat gacggcctgc agcggtacgt cgaaggcatc gacttcacac gcgacaccgt
     3601 attcgtcgcc accgatgcag agttgcacgt cgtcggcgcc gcgcatgtcg cgcgcgacgg
     3661 cgagcatgcc gaactcggcg tttcggtcct gccgcaagca cggggccacg gcatcggcgc
     3721 cgcgttgctg gagcgcagcg cgatccacgc gcgcaatgcc ggcgtgcgcg tgctgttcat
     3781 gaactgcctg gtcgaaaacg caccgatgct tcacctggca cgcaagcagg gcctgcaggt
     3841 ggcggtctcc agcggcgaag cggaagcttt cgtacgcttg ccgcgaccca gcctgcgcag
     3901 cctggccagc gaggcagttg ccgatcatct ggggctgatc gatcacgcgc acaaggcgca
     3961 ctgggtggcg ctgcgcgggc tgtggctcgc gtcgttcggc ggcgagtcgg agccgccgag
     4021 aggcggcagt gaaacgcaag aaggaagcag ctgacatggt caccacatcg aagaagagca
     4081 agcgcacggc tccgcaggcc gtgggcgaac tgccgctgct gtcccttgcg cgccaggtgc
     4141 gcagttggac cgacacgctg ctcggcgtcg ccggcagcgc gggcgacatc ggcatgacac
     4201 tcgcgcaggc gcgcatccgc gacccggaga agaaggctgc ggtgggcaag gccggatcgc
     4261 aactgcgcca gtggcgcgag gaagccgggc tgacggccaa ggaactcagc gatgctgtcg
     4321 gcctgggcga cacaaagctg atcgagcagg ccgagggcgg cacggctgcg ctgcccttcg
     4381 aaatcgtgct gcgactcgca ggcgtgctcg gccggcgcga cccgatcccg gttgcgatgg
     4441 gattgacccg ccagtacaac cccgagttgt ggaaggcgct tgagtcgatc ggcatcgggc
     4501 gcctcgcggt gcagggcaca cgcgagcgcg aactggccaa tgtctaccgt ggcaacgatg
     4561 cggcgcgtgc gctcgacgac gaggcctttg cgcaggtact cgccttcacg aagcaggcct
     4621 tcgacatggc agtggacttc tcgcgccggc cggcccgcgc caagacaaag cccggctgat
     4681 ctcgcgggtg gtcgcggcac tggtcgtttc cgcccgtcgg agggctgctg acgatccggt
     4741 ccgtcaaggc gatccgccgg gccacggcgc tgctggtcga cgacctggtg gacgaaggcg
     4801 tgctgcgctg ggcggcggcg ttctgcacgc atcgtgcacg tcggcaatgg cggtggaggc
     4861 ttctgtgcgc ccaggccgtc gcccgcgatg accgtccgcc gaggggagcg gcgaaccttg
     4921 gggcagccca gccggcttgc cgagcacgcc gcagtccttc atccacaagc tgatgatcgc
     4981 cgaggcgcgg cgcggggcgg cgagcgcgtg gtgtggctga agggggtaat cgtatttttc
     5041 ctgcagattg acacggtgac ccagaaaaac tctagc
//
LOCUS       DAOORV010000232         5013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20105_length_5013_cov_7.019564, whole genome shotgun sequence.
ACCESSION   DAOORV010000232 DAOORV010000000
VERSION     DAOORV010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20105_length_5013_cov_7.019564"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKV56_17940"
     CDS             <1..666
                     /locus_tag="PKV56_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HOX69733.1"
                     /translation="AVGTIKNRMAELRWWAEKIGKQNVIARDNDHYGIGNRQYVTNVS
                     KARQLTGGELARITDPYTAMSLRLQAAFGLRRGESIKIRPEWADRGDRLALKDTWTKG
                     GRPREIPIRNAEQRQVLDEAKALAGRGSLIPAERSYVEQLRRFEYQCAAAGAHRIHGH
                     RHQYAQTRYRELTGWPAPAAGGPRSRDLTPSQREADREARLTISEELGHEREQITAVY
                     LGR"
     gene            complement(674..1081)
                     /gene="merR"
                     /locus_tag="PKV56_17945"
     CDS             complement(674..1081)
                     /gene="merR"
                     /locus_tag="PKV56_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HOX69734.1"
                     /translation="MHDIAGATIGALAEAAGVNVETIRFYQRKGLMPEPEKPYGSIRR
                     YGTAELARVRFIKSAQRLGFSLEEVGELLKLEDGARCGEARQLAEQKLVDVRQRLTDL
                     QRIESVLAGLVARCSAVRGRVNCPLIASLQEVA"
     gene            1154..1504
                     /gene="merT"
                     /locus_tag="PKV56_17950"
     CDS             1154..1504
                     /gene="merT"
                     /locus_tag="PKV56_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HOX69735.1"
                     /translation="MAEPTQGRSALFAGGLAAILASTCCLGPLVLITLGFSGAWITNL
                     TVLEPFRPVFIGAALVALFFAWRRIWRPAATCQPGEACALPAVKTTYKALFWLVAALV
                     AIALGFPFIAPWFY"
     gene            1517..1804
                     /gene="merP"
                     /locus_tag="PKV56_17955"
     CDS             1517..1804
                     /gene="merP"
                     /locus_tag="PKV56_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HOX69736.1"
                     /translation="MKRALLAFATVVTMSGPAWSAPKTVTLSVPDMNCAACPITVKKA
                     LGKVAGVAKTEVNLDKREATVTFDDARTNVDALTRATRDAGYPSTLVGSAK"
     gene            1801..2043
                     /locus_tag="PKV56_17960"
     CDS             1801..2043
                     /locus_tag="PKV56_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HOX69737.1"
                     /translation="MSEVILQSTITCPNCGHAKTETMPTDACQWFYECEQCHSVLKPK
                     PGDCCVYCSYGSVPCPPIQQRGAGNQHCCTSETRVE"
     gene            complement(2199..3263)
                     /locus_tag="PKV56_17965"
     CDS             complement(2199..3263)
                     /locus_tag="PKV56_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HOX69738.1"
                     /translation="MKSGRSLVDLAHELERQISSKRDLVVPSSFLQCRTDEDGSLKLI
                     VDSRQGDGEYGVTGLARRQLADKLKIPFAYFERMRTEQPQLLDRNVNTWLQSDGDRRM
                     IRTLDGQVRAVLSDRYRRLDNFDLAENVLPILQRLEGARFESVELTETKMYLKVITPR
                     VEFEVAPGDIVQAGIVITNSEVGCGTLSVQPLIYRLVCKNGLIASDHALRKTHVGRSL
                     TTEAESVNVFRDDTLAADDRAFFLKVRDVVEAAVSEATFRQVAQKLQKTRDIRLTGDP
                     VKSVEVLANRYTLNDTERAGVLRHLIVEGDLSAYGLVNAVTHFSQDVEDYDRATEFEA
                     LGGKLIELAPGDWKELAHAA"
     gene            complement(3398..3991)
                     /gene="radC"
                     /locus_tag="PKV56_17970"
     CDS             complement(3398..3991)
                     /gene="radC"
                     /locus_tag="PKV56_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX69739.1"
                     /translation="MCRPIESMSDAALLSTLVGPRTAANLLKEASGSLSRLLNADVAQ
                     QVPSSSVGAKLIAARELVRRALAETMCERDMLASPAAVRDYLRIMLAQRDHEVFMVLF
                     LDAQNRVIAPEEMFRGTLTQTSVYPREVVKRALSLNAAAVILAHNHPSGVAEPSRADE
                     FLTQSLRSALALVDIRVLDHIVIAGSNATSFAERGLL"
     gene            complement(4080..4520)
                     /locus_tag="PKV56_17975"
     CDS             complement(4080..4520)
                     /locus_tag="PKV56_17975"
                     /inference="COORDINATES: protein motif:HMM:NF023523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HOX69740.1"
                     /translation="MSESSQASSPSSFFNLHVEGVGYLNRVRTVKPKKGQEFLACTVA
                     ALRGSDSDVCYTKFDCRVSGADAQAIVKRLENDVAADKAVIIGFRIADIYPEMFTFEK
                     GDRKGQPGVSIKGRLLRIKFAKVNGEPIDVPQPARSEERESVPF"
     gene            complement(4640..>5013)
                     /locus_tag="PKV56_17980"
     CDS             complement(4640..>5013)
                     /locus_tag="PKV56_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69741.1"
                     /translation="PAVRHPSAQECRMLRVLVQDALAAALLHGFERVSFHGFCIEARC
                     LSASGADPACMALTLTADGGAEMLDRVRIAVAFRSDPGSACRIDHSHAPATAARRAES
                     LAGCVSPVDARYASPETGQIS"
BASE COUNT          885 a         1611 c         1667 g          850 t
ORIGIN      
        1 gccgtgggca cgatcaagaa ccggatggcg gagctgcgat ggtgggccga gaagatcggc
       61 aagcagaacg tcatcgcccg cgacaacgac cactacggca tcggcaaccg acagtacgtc
      121 accaacgtca gcaaggctcg gcaactgacc ggaggcgagc tcgccaggat cacggatccg
      181 tacacggcga tgtcgctgcg gctgcaggcg gccttcgggc tgcggcgcgg cgagtcgatc
      241 aagatccggc cagagtgggc cgaccgtggc gaccggctgg cgctcaagga cacgtggacc
      301 aagggtgggc ggccgcgcga gattccgatc cgcaacgcgg agcagcgcca ggtcctcgac
      361 gaggccaagg cactggccgg caggggcagc ctcatcccgg ccgagcgaag ctacgtcgag
      421 cagttgcgcc gcttcgagta tcagtgcgcg gcggccggcg cgcaccgcat ccacggccat
      481 cgccaccagt acgcgcagac ccgctaccgc gagctgaccg gatggccggc gccggcggcc
      541 ggcgggccgc gatcgagaga cctcacgccc tcgcagcgcg aggccgaccg cgaagcgcgg
      601 ctcacgatca gcgaagagct ggggcacgag cgcgagcaga tcacggcggt gtatctcggc
      661 cggtgataga tggctacgcc acctcctgca gcgacgcgat cagtggacag ttgacccggc
      721 cgcggacggc cgaacatcgc gccaccagtc ccgccagcac cgactcgatg cgctgcagat
      781 cggtgagcct ctggcgcacg tccacgagct tctgttcggc cagttgtcgc gcttcgccgc
      841 agcgtgcgcc gtcctcaagc ttcagcaact cgccgacttc ctccaggctg aacccgagcc
      901 gctgcgcgga cttgatgaac cgaacccgtg caagctcggc cgtgccgtat cggcggatgc
      961 tgccgtaggg cttctcgggc tcaggcatca ggcccttgcg ctggtagaag cggatcgtct
     1021 cgacgttgac cccagcggct tccgccaggg caccgatggt tgcgccggct atatcgtgca
     1081 tgggccttga ctccgtactt atgtacggca gtaaggttac accgctgatc acattggtcg
     1141 taaggagtat tcgatggccg aacccacgca gggccgcagc gccctgttcg ccggcgggct
     1201 ggccgcgatc cttgcttcca cctgttgcct cgggccgctg gtgctgatca cgctcgggtt
     1261 ctccggcgcg tggatcacca acctgacggt gctcgaaccc tttcggccgg tcttcatcgg
     1321 cgcggcgctg gtagccctgt tcttcgcttg gcgccgtatc tggcgccccg cagcgacatg
     1381 tcaaccgggc gaagcctgtg cgttgccagc ggtgaagacg acctacaagg cgctgttctg
     1441 gctggtcgcc gcgctcgtgg cgatcgcgct cggctttccc ttcatcgccc cctggttcta
     1501 ttgagaggac aacaccatga agagagcatt gctcgcgttt gccaccgtcg tcacgatgtc
     1561 cggtcccgcc tggtccgcac ccaagaccgt gacgctgtcg gtacccgaca tgaattgcgc
     1621 ggcctgtccc atcacggtga agaaggcgtt gggcaaggtg gccggtgtcg ccaagactga
     1681 ggtcaacctg gacaagcgtg aggcgaccgt cactttcgac gacgcccgca cgaatgtgga
     1741 tgccctgacc cgtgcgacgc gggatgccgg ctatccctcc acactcgtgg ggagcgccaa
     1801 gtgagcgagg tgatcctgca atcgaccatc acttgcccga attgcggaca tgccaagacg
     1861 gagaccatgc cgacggacgc gtgtcaatgg ttctacgagt gcgagcaatg ccactccgtg
     1921 ctgaagccga agccaggcga ctgctgcgtc tactgctcct atggctcggt gccttgcccg
     1981 ccgatccagc aacgtggcgc aggaaatcag cattgctgca cgagcgaaac acgcgtcgag
     2041 tgagtcggta gcaccgtgct cgccgaacac gtccgtcagc actgctgaca gacgaaaaaa
     2101 tgcccgggca gccgtgaggc tgcgccgggc gaggggggag gtcttcaatc aggggcaaga
     2161 gtcccgaagc accgccggcg gcagtgcttc gggggttctc aggcggcgtg ggccaattcc
     2221 ttccagtcgc caggggccag ctcgatgagc ttgccgccca gcgcctcgaa ctcggtggca
     2281 cggtcgtagt cctcgacgtc ctgcgagaag tgcgtgacgg cgttgaccag cccataggca
     2341 ctgagatcgc cttcgacgat gaggtgccgc agcacgccgg cccgctcggt gtcgttgagc
     2401 gtgtagcggt tggccagcac ctcgacggac ttcaccgggt cgccggtcag acggatgtcg
     2461 cgcgtcttct gcagcttctg cgcgacctgg cggaacgtgg cttcggacac cgcagcctcg
     2521 accacatccc gcaccttcag gaagaaggcc cggtcgtctg cggccagggt gtcgtccctg
     2581 aacacattga ccgactccgc ttccgtggtc agcgagcgcc cgacgtgcgt cttgcgcagc
     2641 gcgtggtccg atgcgatcag accgttcttg cacaccagcc ggtagatcag cggctgcacc
     2701 gagagcgtgc cgcagccgac ctccgagttg gtgatgacga tgccggcctg cacgatgtcg
     2761 ccgggggcga cctcgaactc gacgcggggc gtgatgacct tcaggtacat cttcgtctcg
     2821 gtcagttcga ccgactcgaa gcgggcacct tccagacgct gcaggatcgg caggacgttc
     2881 tccgcaagat cgaagttgtc cagccggcga tagcggtccg acagcaccgc cctcacctgc
     2941 ccgtccagcg tgcggatcat tcggcggtcg ccgtccgatt gcagccaggt gttgacgttg
     3001 cgatcgagca gttgcggctg ctcggtacgc atgcgctcga agtaggcgaa cgggatcttc
     3061 agcttgtcgg cgagctgccg acgcgccaga cccgtgacgc cgtactcgcc gtctccctgg
     3121 cgggagtcga cgatcagctt gaggctgcca tcctcatcgg tacggcattg caggaaggac
     3181 gaaggcacga ccaggtcgcg cttggaggag atctggcgct cgagttcgtg ggccaggtcg
     3241 acgagggagc gtccgctctt catggtgagt ctccagagaa gttgcaggaa cggccccccg
     3301 ccatgcgggg atgcacatcc ccgctggggt gaagaaccgg accggggaat cccgatccgg
     3361 atgggaagcg cgacgggtgt cgcgcagggt tcacgggcta caacaaaccg cgttcggcga
     3421 acgaggtggc gttgctgcct gcgatcacga tgtggtcgag cacacggatg tcgaccaagg
     3481 ccagcgcgga gcgcagcgac tgggtcagga actcgtcggc ccggctgggc tcggcgacac
     3541 ctgatgggtg gttgtgcgcc aggatcactg ccgcagcgtt cagcgacagc gcgcgcttga
     3601 ccacttcacg cgggtacacg ctggtctgcg tgagcgtccc tcgaaacatt tcctcgggtg
     3661 cgatgacccg gttctgtgca tcgaggaaca gcaccatgaa gacttcgtgg tcgcgctggg
     3721 ccagcatgat ccgcaagtag tcccgcaccg cggccggtga ggcgagcatg tcccgctcgc
     3781 acatcgtctc ggcaagggca cgacggacca gttccctggc cgctatcagc ttcgctccga
     3841 cggaggacga cgggacttgc tgcgctacgt ctgcgttcag cagccgcgac aggctgccgc
     3901 tcgcttcttt caacaggttc gctgccgttc gaggacccac cagcgtgctg agcagggccg
     3961 cgtccgacat cgactcgatc ggtcgacaca tggagttctc cttcgtatga cgaatgggga
     4021 actcctcccc cagggagcaa gctccccggg ggatggattg agactgacag tgccggcggt
     4081 cagaacggaa cgctttcgcg ttcctctgag cgcgccggtt gcggcacatc gatgggttcg
     4141 ccgttgacct tggcgaactt gatgcgcagc agtcggccct tgatgctgac gcccggctgt
     4201 cccttcctgt cgcccttctc gaaggtgaac atttcgggat agatatcggc gatccggaag
     4261 ccgatgatca cagccttgtc ggccgcgaca tcgttttcca ggcgcttgac gatcgcctga
     4321 gcgtcagcac cgctgacacg gcagtcgaac ttcgtgtagc agacgtcact gtcgctgcca
     4381 cggagggcag ccacggtgca ggccaggaac tcctggccct tcttcggctt cacggtgcga
     4441 acacgattga gatagccgac gccttcgacg tgcaggttga agaacgacga cgggctgctg
     4501 gcttgggatg actcggacat gatggcctcc tatgacatgg aacacatgaa aatggagaga
     4561 ccatccccag tgcggggatg ggactccccg gctgggtaga agcactgacg aaatctccgg
     4621 gccttgcggc cgccggttct caggagatct gaccggtttc gggcgatgcg taacgggcat
     4681 caaccgggct gacacagcca gcgagagact cggcgcggcg cgcagcggtc gctggcgcat
     4741 gcgagtgatc gatgcggcat gctgatcccg ggtccgatcg gaatgcaact gcaatgcgga
     4801 cgcgatccag catctcggcg ccgccgtcgg cggtgagcgt gagtgccatg caagccgggt
     4861 ccgcgccgga tgcggagagg cacctggcct cgatgcagaa gccgtggaaa gagacccgct
     4921 cgaagccgtg cagcagcgcg gcggcgagtg cgtcttgcac caggacgcgg agcatccggc
     4981 actcctgcgc gctgggatgc cgaaccgccg ggc
//
LOCUS       DAOORV010000233         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20189_length_4996_cov_11.603926, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000233 DAOORV010000000
VERSION     DAOORV010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20189_length_4996_cov_11.603926"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKV56_17985"
     CDS             <1..856
                     /locus_tag="PKV56_17985"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX69742.1"
                     /translation="SPLKNRGVLFRQAGPLLYTQRGDIFLNLHPDRLEVVNPGRLPLG
                     VTPQTILHASRRRNAGLARVFHDLGLMEREGSGFDLMYEQLLAADRPPPVPTEGVDSV
                     TVTVQRRKPRLQIVALIDDADQRFQLSQRERITLGILAQADGLTAIALCKQVEVADAD
                     ALRPWLGRLLALGLVHTTGRTKGQRYFVAPTLLRGAKVPTVTTLARIEPHRLRALILE
                     DLARYPQSASSEINARVGPELSVKTIKRALDELVDAGQVTPEGERRWRRYRLWSIGQT
                     TAESVAAR"
     gene            1132..4647
                     /locus_tag="PKV56_17990"
     CDS             1132..4647
                     /locus_tag="PKV56_17990"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOX69743.1"
                     /translation="MAYTDVNSEDRLVQATFAEHLEHTLGWESVYAWNQETFGPDSLL
                     GRRDTRDVVLVRDLHAALVRLNPELPAAAIDDAVAQLTQHDPTRSLLQHNRSFHALLR
                     DGVPVRWREGAGQLRQTQARVIDFRDPLNNRFLAVRELKITGLRAPSYNRRADLVCFV
                     NGLPLVFIELKAVYLNVRAAYEGNLKDYLDENVIAHAFHHNAFIVVSNGHAARFGSVT
                     SAWDHYAEWKRLDETDKGSVAAEVLLNGMLARSRLLDLLENFILFDESRAGAVRKVVA
                     RNHQVMGVNRAVAAVVRQEELKAQFPPGERLRHRVVTIPLVEPAAVNADAPSDAANAP
                     SLAVLHAAEGGPRGRPPKEITVIERVHPDLGRLGVVWHTQGSGKSYSMLFFAEKVRRT
                     VPGNFTFLLMTDRNDLDSQIYKTFIGCGVVPPSTPRAESGEALRELLSQNHRYVFSLI
                     HKFNQDVAPDKPYSERDDIIVISDEAHRTQAGRLARNMRMALPNAAFIGFTGTPLFKN
                     DHLTKRIFGGYVSRYDFKRSEEDGATVRLVYENRGDKLSVARKDLNEKIAAAIEQAEL
                     DSDQQAKLQKLLGTDYEVITADKRLLTIAQDFVDHCAARWEAGKAMLVCIDKVTCGRM
                     LMMARPLWLAKAAAVHQAAHDKQAEIDATADADLQQRLHALRDRLLAQAKWLDETIIG
                     LIVSEAQGEVAEFKEWGIDIVPHRTLMKQGFETPDGKRVDVETAFKDPKHHFRVAVVC
                     AMWLTGFDVECLSTLYIDKPMKAHTLMQAIARANRRFPGKDFGLVVDYNGMLASLRAA
                     LAQYALGDDNSGAAEIVAPLQERVAALDDAITEAEVFLRARGFEPDRLIGAKGWVRIA
                     ALADAVEAVNAPLPPAAGTVLAAGAPNDKPAGADHSRQRFEILARAIFLRFKALVTEP
                     TVHLYADRHDNIEAIYKKLSEKRDDADITDLLKALHRIVNKAIATDAPGQDQAHGLTV
                     DLSLIDLEKLRDEFAKKVKHKATAIEDIRALIERKLALLLAQNPTRMNYDIKYREIVA
                     AYNLDKDRATIEETFGKLMAVSQGLDDEQRRHVREGLSESELAIFDLMQKSTLAATER
                     ERIKQSSRELLAELLRIIAPLEQWTEKEQTRAEVETAILDQVFMLPEPPYSADDKDVM
                     AKLLYEHVWQQSRGGRFGPEPPRAS"
BASE COUNT          972 a         1560 c         1675 g          789 t
ORIGIN      
        1 cagtccactt aaaaatcggg gcgtgctgtt cagacaagcg gggccacttc tctacaccca
       61 acgcggcgac atcttcctga acctacaccc cgaccggctg gaagtggtga accccggccg
      121 cctgccgctg ggcgtgacac cgcagaccat cctgcacgcc agccggcgcc gcaacgccgg
      181 cctggcccgc gtgttccacg acctgggcct gatggagcgt gagggcagcg gcttcgacct
      241 gatgtacgag cagttgctgg ccgccgaccg cccgcccccg gtgcccaccg agggagtgga
      301 ttcggtgacg gtgacggtac agcgacgcaa accccgcctg cagatcgtgg ccctgatcga
      361 cgatgctgac cagcgcttcc aactgagcca gcgcgagcgc atcacgctgg gcatcctggc
      421 gcaggccgat gggctgacgg ccatcgccct gtgcaagcag gtggaggtgg cagacgccga
      481 cgcgctcagg ccgtggctgg ggcggctgct ggcgctgggc ctggtgcaca ccaccggccg
      541 caccaagggc cagcgctatt ttgtggcgcc cacgttgttg cgcggggcca aagtgcccac
      601 ggtgacgacg ctggcgcgga tcgagccgca caggctgcgc gcattgatcc tggaagacct
      661 ggcgcgctac ccgcagtcgg ccagttccga gatcaacgcc cgggtggggc cagagctatc
      721 ggtgaagacc atcaagcggg cgctggacga actggtggac gcggggcagg tcacgccaga
      781 gggtgaacgg cgctggcggc gctatcggct gtggtctata ggacaaacga ccgccgaatc
      841 ggtagccgcg cgctgacaag ccctatcgcc caccgccgca agtggttgat tcaaataggg
      901 aaagtaaagg acactcgccg gcgtatgtcc gtgaacggcg cctcggctgt cctttaagtg
      961 gccgctgtgt gcggcaaatc gaccaagcgc gccgggcggt ctgatcaaca ggggcaggga
     1021 gcagtgatgt cagaccagcg caaagcacgc aacaggctcg acaccgaccg gatggccgcc
     1081 gacgtggcgg ttggtgctgc acggccacgg tcgcgctgac cacccgcttc catggcctat
     1141 accgacgtca acagcgaaga ccgcctagtg caggccacct tcgccgagca cctggaacac
     1201 acgttgggct gggagagcgt ctacgcctgg aaccaggaga ccttcggccc cgacagcttg
     1261 ctgggccgca gggacacgcg ggatgtggtg ctggtgcgcg acctgcacgc tgccctggtc
     1321 aggctgaacc cggagctgcc ggcagccgcc atcgacgacg cggtggccca gttgacacag
     1381 cacgacccaa cgcggtcgct gctgcagcac aaccgcagct tccatgcctt gctgcgtgat
     1441 ggtgtgcctg tgcgctggcg cgagggtgcc ggccagcttc gccagactca ggcccgggtg
     1501 atcgacttcc gcgacccgtt gaacaaccgc tttctagccg tgcgtgagct gaagatcacc
     1561 ggcctgcgcg cacccagcta caaccggcgc gccgatctgg tgtgcttcgt caacggcctg
     1621 ccgctggtct tcatcgaact gaaggcggtg tacctgaacg tgcgcgctgc ctacgaaggc
     1681 aacctgaagg actacctgga cgagaacgtc atcgcccatg ccttccacca caacgccttc
     1741 atcgtggtca gcaatgggca tgcagcgcgt ttcggctcgg tcaccagcgc ctgggaccac
     1801 tatgccgaat ggaagcggct ggacgagacc gacaagggca gcgtggcggc tgaggtgctg
     1861 ctgaacggca tgctggcgcg cagccgcttg ctggacctgc tggaaaactt catcctgttc
     1921 gacgaaagtc gcgccggtgc cgtgcgcaag gtggtggcgc gcaaccacca ggtgatgggc
     1981 gtcaaccggg ccgtggcggc cgtggtgcgg caggaagaac tgaaggcgca gtttccgccg
     2041 ggtgaacggc tgcgccaccg cgtggtgacg attccgctgg tcgagccagc ggccgtgaat
     2101 gcggacgccc cttccgatgc cgccaacgcc ccatcgctgg cggtgttgca tgccgctgag
     2161 ggcggcccac gcgggcggcc gccaaaggaa atcaccgtca tcgagcgtgt gcaccctgat
     2221 ctggggcggc tgggcgtggt gtggcacacc cagggcagcg gcaagtcgta ctcgatgctg
     2281 tttttcgccg agaaggtgcg gcgcacggtg cctggcaact tcaccttttt gctgatgacc
     2341 gaccgcaacg acctggacag ccagatctac aagaccttca tcggctgcgg cgtggtgccg
     2401 ccttccaccc cacgggcaga gtcaggcgaa gcgttgcgag agctgttgag ccagaaccac
     2461 cgctatgtgt tcagccttat tcacaagttc aatcaggacg tggcgcctga caagccctac
     2521 agcgagcgcg acgacatcat cgtgatctct gacgaggcgc accgcacaca ggctggcagg
     2581 ctggcgcgca acatgcggat ggcgctgccc aacgcggcct tcatcggctt caccggcacg
     2641 ccgctgttca aaaacgacca tctgaccaag cgcatcttcg ggggttacgt ctcgcgctat
     2701 gacttcaagc ggtcggagga ggacggcgcc accgtgcgcc ttgtctacga gaaccgcggc
     2761 gacaagctga gcgtggcgcg caaggacttg aacgaaaaga ttgcggcggc catcgaacag
     2821 gctgagctcg attctgacca gcaggccaag ctgcagaagc tgctgggcac ggactacgag
     2881 gtgatcaccg ccgacaagcg gctgctgacg attgcgcagg actttgtcga ccactgcgcc
     2941 gcacggtggg aggcaggcaa ggcgatgctg gtgtgcatcg acaaggtgac ctgcgggcga
     3001 atgctgatga tggcgcggcc gttgtggctt gcaaaggctg ccgctgtgca ccaggcagcg
     3061 cacgacaagc aggccgagat cgacgccaca gccgatgccg atcttcagca acggctgcat
     3121 gccctgcgtg accgcctgct ggcccaggcg aagtggctgg acgagaccat catcgggctg
     3181 atcgtcagcg aggcccaggg ggaagttgcc gagttcaagg agtggggaat cgacatcgtc
     3241 ccgcaccgga cgctgatgaa gcagggattt gagacccccg acggcaagcg ggtggacgtg
     3301 gaaaccgcat tcaaggaccc caagcaccac ttccgcgtgg ccgtggtgtg tgcgatgtgg
     3361 ctgacaggct ttgacgtgga atgcctgtcg acgctgtaca tcgacaagcc catgaaggcg
     3421 cacacgctga tgcaggccat cgcgcgtgca aaccggcgct ttccgggcaa ggactttggc
     3481 ctggtagtgg actacaacgg catgctggcc agcctgcgtg ccgcgctggc gcagtacgcg
     3541 ctgggtgacg acaacagtgg ggcggccgag atcgtggcgc cgctgcagga gcgggtggcg
     3601 gcgctggatg acgcgatcac cgaggcagag gtttttctgc gggcgcgtgg ctttgagccg
     3661 gaccggctaa tcggggccaa aggttgggtg cggatcgccg cgctggccga cgcggtggaa
     3721 gctgtcaacg cgcccttgcc gccagcggcc ggcaccgtgt tggcggcggg ggcgccaaac
     3781 gacaagccgg cgggtgctga ccactccagg cagcgtttcg agatcctggc ccgggcgatc
     3841 ttcctgcggt tcaaggcgct ggtgacggaa cccacggtgc acctctacgc cgaccgacac
     3901 gacaacatcg aggccatcta caagaagctg agcgagaagc gcgatgacgc agacatcacc
     3961 gacttgctga aagccttgca tcgcatcgtc aacaaggcga tcgcgacgga tgccccaggc
     4021 caagaccagg cccacggact gaccgtggac ctgtcgctca tcgacctgga aaagctgcgc
     4081 gacgagttcg ccaagaaggt gaagcacaag gccacggcga tcgaggacat ccgggcgctg
     4141 atcgagcgca agctagcgct gctgctggcg cagaacccaa cgcggatgaa ctacgacatc
     4201 aagtaccgcg agatcgtcgc cgcttacaac ctggacaagg accgcgcgac catcgaggag
     4261 acgttcggga agctgatggc ggtgtcgcag ggcctggacg acgagcagcg ccgccatgtg
     4321 cgcgagggct tgagcgaaag cgagctggcg atcttcgacc tgatgcagaa gtccaccctg
     4381 gccgccactg agcgcgagcg catcaagcag tcaagccggg aactgctggc ggagttgctg
     4441 cgcatcatcg cgccgctgga gcaatggacc gagaaggagc agacaagggc cgaagtcgag
     4501 acggccatcc tcgatcaggt tttcatgctg cccgagccgc cgtattcggc ggacgacaag
     4561 gatgtgatgg caaagctgct gtacgagcat gtttggcagc agagtagggg tgggaggttt
     4621 ggcccagagc cgccgcgcgc cagctagtgg agccgttcac gcacgccgca ctggtctcgt
     4681 tgcagaccag cggcgtccac cccaggaata ggtggcagct ttacgtcgcc aagctgccgc
     4741 tggcttgcga ccgctggtcg gcagcacgcg gccacttcct gtcatacgac tgactaggaa
     4801 gtcgcgatgc caatgaccgg tttgaaggac agccgccata ggacgacggc cacttgaacg
     4861 agttcattcg ccaaagcggt cgctcacggt actaccgcaa taccgatcca cagccgatga
     4921 atacgtacgt cagcaggtcg actagtgtag tgtttcgttc ttgagggaac ctatatgagg
     4981 ccgtccgctc caatgc
//
LOCUS       DAOORV010000234         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20402_length_4949_cov_12.816510, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000234 DAOORV010000000
VERSION     DAOORV010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20402_length_4949_cov_12.816510"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKV56_17995"
     CDS             <1..392
                     /locus_tag="PKV56_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOX69744.1"
                     /translation="NMREVVWDKGPVQCGVVLRGDGHTARRWHDSEVAAIGYWLQQML
                     AKRGFLDAQGRQVPVLEMSARLSRQLELQLEGDAESAARGQNGTLLTTTSFVTRGRQC
                     PECGARALRRVDGCERCTECNHVGSCG"
     gene            479..1495
                     /locus_tag="PKV56_18000"
     CDS             479..1495
                     /locus_tag="PKV56_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOX69745.1"
                     /translation="MNHDFEARVSDVKSRAHGHWTGILRSLGVDEDILKGKNLPCPID
                     GCGGTDRFQYTDKFGEGNYHCRSCGAGGGLKLAHAVRGGRFSDLLDDIEALVGSVRLA
                     TSSGLAGPSPERMKRLCHQTWTEAKPVAIGDEVDRYLRGRGINQGAYPRTLRFHPSLG
                     YYEKQPGQKRSKLIASYAAMIACVQGPDGHAVTLHRTYLRDGRKALGDQSKKLLSAGI
                     NGAAVRLDEATDELALTEGIETALAVRLRTGKPTWAAINCGNLERIWIPEQVQRVCIY
                     ADNDSDAEFAGQASAYILAQRLVREAKKAGIHRTVEVFVPRQAGTDWADIWLASLAKD
                     KRAA"
     gene            1746..2810
                     /locus_tag="PKV56_18005"
     CDS             1746..2810
                     /locus_tag="PKV56_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HOX69746.1"
                     /translation="MKYGRSLVDLATELERQLATKQDMIVPTPLMHHVTSENGTSVLN
                     IETHDGVRTFRTTENCRRQLADRLKIPFAYFERMRTDQPALLDRNIDTWLHAQPEQRM
                     VRTLDGNARAFLSDRYRRLDNYDLLAHVYPMLRELPGARVESCEVTDSRMYLKVVTSR
                     VQFELAPGDVVHAGVVISNSETGQGSLSVSPLVFRLLCRNGLIAADQAMRKTHIGRMT
                     ETSSDEVTFFKEDTLAADDAAFFLKVRDTVQAAVSQATFSLIAERMRKTMGIKLVGDP
                     VKSVERLAVKYLLQEHEKAGVLRALIKDGDLTGFGLVNAVTGYAQEVDQYDRSTELEA
                     IGGRMLDQAAKEWTELAEAA"
     gene            3161..4003
                     /locus_tag="PKV56_18010"
     CDS             3161..4003
                     /locus_tag="PKV56_18010"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX69747.1"
                     /translation="MAAEHSEEDRHRALAAFIESMLDRHGVPERQRIRALEAAIGISY
                     TQVRRRMIGEAPWTIDEIKKLASHFHEPLIPIMSALADAPGIPATLPFGGAAMPCTIW
                     PVGEPLPGRIGPMVAMHDSTTDQWTVAPAAEVSDSPSYELARLLFERAPPRRVAVLDD
                     DRDSAQSIVDFLGAKGLDAQPFNCAIDLLAAMEGQPFDGFVVDWLLGDNTAKDLLSKI
                     RARVPSGPVVILTGQIATGAASEDELVMVGASYRALLFEKPTRPLSIFNALQVGFTSP
                     TTPG"
     gene            4401..4652
                     /locus_tag="PKV56_18015"
     CDS             4401..4652
                     /locus_tag="PKV56_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69748.1"
                     /translation="MRDAAMRDMGDAVCSFGLARQDFAAIMSRAPDELLSFVCGIGDQ
                     ALFLPRSDLSSLLALPPGIAPAIASARSATARATRVADA"
     gene            4673..>4949
                     /locus_tag="PKV56_18020"
     CDS             4673..>4949
                     /locus_tag="PKV56_18020"
                     /inference="COORDINATES: protein motif:HMM:NF017124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="HOX69749.1"
                     /translation="MGSPSAYSLRALETARECVILGARPRTIAHLTGLTPGYILRAVY
                     CERYPAPIGRPQYSEDFFFKTTRRLQAEACFLATHYRRLTREGFMQAD"
BASE COUNT          906 a         1701 c         1527 g          815 t
ORIGIN      
        1 agaacatgcg cgaggtggtc tgggacaagg ggcccgtgca atgtggcgtc gtcctacgtg
       61 gcgatggtca caccgccagg cgctggcatg actccgaggt cgccgcgatc ggctattggc
      121 tgcagcagat gttggccaag cgcgggttcc tggatgccca aggcaggcag gtgcccgtgc
      181 tcgagatgtc tgcaaggctg tctcggcaac tggagctgca gttggagggc gacgctgaat
      241 ccgctgctcg cggccagaac ggcacgctgc tcacaaccac cagcttcgtg acccgcggcc
      301 gccaatgccc cgagtgcgga gcgcgcgcgc tgcgacgcgt ggatggctgc gaacgctgca
      361 ccgagtgcaa ccacgtcggc agctgcggct gaatcttcca acgaagcgct gaggtgcgcg
      421 gaggcccgcc cttgtggcgg gcttccggcc acccagcctc aacccatcgg acaagaccat
      481 gaaccatgac tttgaagcgc gcgtgagcga cgtgaagtcc cgcgcgcacg ggcattggac
      541 gggcatcctg cggtcgctgg gcgtcgacga agacatcctc aagggcaaga acctgccctg
      601 cccgatcgac ggctgcggcg gcaccgaccg cttccagtac accgacaagt tcggcgaagg
      661 caactaccac tgccgttcct gcggtgcggg tggcggcctg aagctcgcgc acgcagtacg
      721 tggcggacgg ttcagcgact tgctcgacga catcgaagca ctcgtcggtt cggtgcggct
      781 ggcgacatcg tccggcctgg ccggtccgtc gccggagcgg atgaagcggc tgtgccacca
      841 gacctggaca gaggccaagc ctgttgccat cggcgacgaa gtcgaccgct acctgcgcgg
      901 ccgcggcatc aaccaaggcg cctaccccag gacgctgcgg tttcatccgt ccctgggcta
      961 ctacgagaag cagcccgggc agaagcgctc gaagctgatt gccagctacg ccgcgatgat
     1021 cgcgtgcgtg cagggcccgg acggccacgc cgtcaccctg cacaggacct acctgaggga
     1081 cggccgcaag gccctgggcg accagtccaa aaagctgctg tccgcaggca tcaacggcgc
     1141 ggccgtgcgt ctcgacgagg ccacggacga actcgcgctc accgagggga tcgagaccgc
     1201 gctggcggtc cgtctgcgga ccgggaagcc cacctgggcg gccatcaact gcggcaacct
     1261 ggagaggatc tggatccccg agcaggtgca gcgtgtttgc atctacgcgg acaacgacag
     1321 cgacgcggag ttcgctggcc aggcctcggc atacatcctc gcccagcgcc tcgtgcgcga
     1381 agcgaagaag gccggcatcc atcgcacggt cgaagtcttc gtgccccgcc aagcgggcac
     1441 ggactgggcc gacatctggc tcgcctcgct tgccaaggac aagcgcgcgg cgtaggcttt
     1501 cgggggtggc agcaatgcca ccctctcttt cggtgtgccc cgggcgggca cagcgacaga
     1561 ggtttcccaa gtggcagctg ccaggccacg ctgaactcca gcgaaggtag cccttcaatc
     1621 ccagtgcacg acggtcaccg ttcgtgccgc atcgccgctc agccacgctg agcccacacc
     1681 cccgcgggga cgcgcttccc cgctgcaggg gtagagccgt ccccgtattt catttggaga
     1741 tctccatgaa gtacggacgt tccctcgtcg acctcgccac cgaactcgag cggcagctcg
     1801 cgacgaagca ggacatgatc gttccgaccc cgctgatgca ccacgtcacc agcgagaacg
     1861 gcacgtcggt cctcaacatc gaaacgcacg acggcgtgcg caccttcagg acgaccgaga
     1921 actgccgccg gcagctcgcc gaccgcctga agatcccgtt cgcctacttc gagcgcatgc
     1981 gcaccgacca gcccgcgctg ctggatcgca acatcgacac atggctccac gcgcagcccg
     2041 agcagcgcat ggttcgcacg ctcgacggca acgcgcgggc gttcctgtcg gaccgctacc
     2101 gccggctgga caactacgac ctgctggcgc atgtttaccc catgctgcgt gaactgcccg
     2161 gcgcccgggt cgagtcctgc gaagtcaccg acagccgcat gtacctgaag gtcgtgacgt
     2221 cccgcgtcca gttcgaactg gcgcccggcg atgtcgtgca cgccggggtg gtcatcagca
     2281 actcggagac cggccaaggc tcgctgtcgg tgagcccgct ggtcttccgg ctgctgtgcc
     2341 gcaacggcct catcgcggcc gaccaggcca tgcgcaagac ccacatcggg cggatgaccg
     2401 agacctcgtc cgacgaggtc accttcttca aggaggacac gctggccgcc gacgatgccg
     2461 cgttcttcct gaaggttcgc gacaccgtgc aagcggccgt ctcgcaggcc accttttcgc
     2521 tgatcgccga gcgcatgcgc aagacgatgg gcatcaagct ggtcggcgac ccggtcaaga
     2581 gcgtcgagcg cctggcggtg aagtacctgc tgcaggagca cgagaaggcc ggtgtgctgc
     2641 gagcactcat caaggacggc gacctgactg gcttcggctt ggtcaatgcc gtcaccggct
     2701 acgcacagga ggtcgaccaa tacgaccgtt ccaccgagtt ggaagccatc ggcggacgca
     2761 tgctcgacca agccgccaag gagtggaccg agttggcgga agccgcctga tctgaatcga
     2821 acgaccgctg cggtcgtcac tagcccccag ggacgtcgca accgcggcgt ccctattttt
     2881 tcgcctgctt gccacttcta cccgtgcaca acggttcgca aggacataag cttcttcttc
     2941 ggggtaggtt cgtcggtacg ccgactgccc acagcatcgc tccgtgttcc gtgcccgccg
     3001 aaggcgacga tcgctgcgaa atctagtagc atccgccggt tgcggggagg gacaaaaaca
     3061 aggaacaacg aaagccaagt aatttggcct tcgtttgcac ctggcggcga cgaatgatga
     3121 ctccgcacgc aaattttccg ctcatcctgt tgggctaggc atggcagccg aacactcaga
     3181 agaagatcgg caccgagccc tcgctgcctt catcgaatcg atgctggacc ggcacggagt
     3241 gccagagcgt cagcgcattc gcgctctcga agcagccatc ggcatctcgt acacccaggt
     3301 gcgccgacgc atgattggcg aggccccttg gaccatcgac gagatcaaga agctcgccag
     3361 ccactttcac gagcctctga tcccgatcat gtcggcactc gccgacgcac caggaatccc
     3421 cgccacgctg ccattcggtg gcgcggccat gccttgcacg atctggcccg tcggcgaacc
     3481 cctacccgga cgcatcggac ccatggtggc catgcacgat tccaccaccg accaatggac
     3541 cgtcgcacca gctgccgagg tctccgacag tcccagctac gaattggcgc gcctgctgtt
     3601 cgagcgcgca ccgccccgtc gggtggccgt tctcgacgat gatcgcgact cggcgcagtc
     3661 gatcgtggat ttcctcggtg ccaagggcct cgacgcccag cccttcaact gcgcgatcga
     3721 tctgctggcc gcgatggagg gccaaccatt cgacggcttc gtcgtcgact ggctgctggg
     3781 tgacaacacc gcaaaagacc tgctctcgaa gatccgcgca cgcgtgccat ctgggcccgt
     3841 ggtgatcctc accggacaga tcgcgacagg cgcggcgagc gaggatgagc tcgtcatggt
     3901 gggcgcgtcc taccgggcct tgctcttcga aaagcccacg cgcccactgt cgatcttcaa
     3961 tgccctgcaa gtgggcttca catccccgac gacgcccggc tgaaccggcc taagggcttg
     4021 aggcgacggc accggccagc aaggccagcg cactgcgggc ccgccttgtc gatgcccatc
     4081 agagctcccg cggacgatga actttttggc gcagtccgca tattcacaat ctgatactga
     4141 cgacataggc attaactaat ttacttccag agtcgtctag tctgttcata agaacttgta
     4201 acgccaataa tcattgacac ctgacgccaa gaatcggtaa tctttcgctc aagtcgccac
     4261 ggcaacgccg tttcgacttg acgctgggtc cgcataccgc cccgcgtcga ccagccacac
     4321 ggcagaccgg gacatagacc atggaccatc caacttctct ctcgcaggtt cagcgagcga
     4381 acctgaacct tctcttgctc atgcgcgatg ccgcgatgcg ggacatgggc gacgcggtgt
     4441 gcagcttcgg gctcgcccgc caggacttcg ccgccatcat gagtcgcgcg ccggacgagt
     4501 tgctgagctt cgtgtgcggc atcggggacc aggccctgtt cctgcctcga agcgacctgt
     4561 cttccttgct cgcgctcccc cctgggatcg ccccggccat cgcctcggcg cgttcggcca
     4621 ccgcgcgggc aacccgagtc gccgacgcct gagtgcgtca cggcggtgac gcatgggcag
     4681 cccttcggcg tacagccttc gcgcgctgga gacggcgcgc gagtgcgtga tcctgggggc
     4741 tcgaccgcgc acgatcgccc acctcaccgg cctcacgccg gggtacatcc tgcgagccgt
     4801 ctactgtgag cgatacccgg ccccgatcgg acgaccgcag tactcggagg acttcttctt
     4861 caagaccacc aggcgcctgc aggccgaggc ctgtttcctc gcgactcact accgccgcct
     4921 gacccgtgaa ggcttcatgc aggcggacg
//
LOCUS       DAOORV010000235         4909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20612_length_4909_cov_6.319942, whole genome shotgun sequence.
ACCESSION   DAOORV010000235 DAOORV010000000
VERSION     DAOORV010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20612_length_4909_cov_6.319942"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..852
                     /locus_tag="PKV56_18025"
     CDS             46..852
                     /locus_tag="PKV56_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX69750.1"
                     /translation="MGWASFNEDNESRYHNATIIRKREHDAMKPESSNGAQAPSKPLT
                     KLKSFAVPQARPLPVIVLADVSGSMAENGKIDALNVALRDMVASFAKEGRLRAEIQVG
                     LITFGGKTAKEHLPLVAAHSIAIIDRFTADGGTPMGAAFDVARELLEDRERIPSRAYR
                     PVLVLVSDGAPTDDWEDALARLQSSERGKKVTRFAMAIGADADKDMLANFANDREAPV
                     FEAHESRDIMRFFRAVTMSVVARSASATPDEPAALTLSEIPDDDLDIGAL"
     gene            870..1562
                     /locus_tag="PKV56_18030"
     CDS             870..1562
                     /locus_tag="PKV56_18030"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HOX69751.1"
                     /translation="MSVTGPGHVSLGQANQDAIALRGWRGGWIAAVADGLGSRPLSGL
                     GSRLAVQAAQQTIRSMPTEEPLRASEPRQTATEFYRRWLRAVPYVDKSCVASTILMAA
                     CGRDGIGRVWQLGDGLVVCRSKGSFSVVTPERLGFGNETRALGIHRAWSDWCTADVAL
                     QERGDAVILMTDGISDDVRIEALEMFTDALVRGVMARTRRGARGWLRNELVNWSTPNH
                     TDDKTVAVIFKG"
     gene            1619..2989
                     /locus_tag="PKV56_18035"
     CDS             1619..2989
                     /locus_tag="PKV56_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOX69752.1"
                     /translation="MQDEFGGIYELKSKLAEGGQGIVLSTDFQNLLVKVSRWPDGDPR
                     TQAWRKQVEALQRMPVVDEGLPVVMPKARIVKPRAGYVMELVDGLIPLELLLKESIES
                     FASGMGLQGFVSSGGLARRLRVLARLARVLAQLHGLGLAHGDISPKNVFVSTSHDQGE
                     VWLIDCDNLTYAVRDSSLQVYTPDYGAPEILRGDAGISTYTDVWSFAVIAFQLLVQLH
                     PFKGGELVDQDSDLEVAALRGELPWIDHRDDARNRSAMGIDREYVLTPLLVELFDRCF
                     RLGVVDPELRPTMGEWAEAFEAATAVQVLCLDSIGCGSTFYWNKAIQCPFCDTKHDPG
                     SVLRLGHAVYAPPAELGEDAAPADSWIATDIVAVLSANELVLRAAPPGTSAYFDSAVA
                     AALQIDGDELVIRAGKSKPLHLQVSGAKSGPALRGTSRIPRDGRVRALHVGPLDATHD
                     VWRFKW"
     gene            2996..>4909
                     /locus_tag="PKV56_18040"
     CDS             2996..>4909
                     /locus_tag="PKV56_18040"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOX69753.1"
                     /translation="MFIHESPLLLPGLLAAQPEDKPIDPSPWRVNEEVLVREAGGELL
                     LRQDSRTLRLRLTPLSGPLEGYAQDQTSIWVLARVKPNLISLRCARPSQSNSLERPLE
                     IGVDQRVADDLHQQRKTSAPTVAAAMQWLTDEFVCERQGFGGDLVFAAILPRAKSGQL
                     QLVGRAWLVDIRRANDGTLLVERVRRRNQKSLDAYAVLVGEIRFVDLAAGIRVQDGDE
                     RAMLEASVSSYGTYLELWQLYSDMEWRREVQTAAELGALRYTRCEPASAEGGAWRFFA
                     DPAELGGFQARWRSLAQQDDASVEAEEAAPDWGDDRYTDLSTSDTHRRFRGAPEFREG
                     TLLAFGRSPIDPPSAGFMFLSLGGDRKQHERRLHARRAIEAGMGVPRLRSLLQDLPIP
                     GQRPSRLPALTPYARQSFKSGKPTAKQELAIQVALNTPDVALIIGPPGTGKTQVIAAL
                     ERRLAEINEGQVIAHEVLISSFQHDAVENALERTSVYGLPAIKVGNRSGASDAVDHWC
                     AARHESVSQRLDQLAIEEPHVPLLQRLNRMVMGLRLTSMPSHQREGAFQKLNELIEEL
                     AQKARLRLSSRWVQDWEEYLARRHCDDLDVVSQLPVGRRRRITRLARSLRVLSESFAD
                     DGAFRAGSLLSEAG"
BASE COUNT          927 a         1441 c         1618 g          923 t
ORIGIN      
        1 cggacccgcc gccgtcatca aaaccattaa acagggagca acgccatggg ttgggcgagt
       61 ttcaatgaag acaacgagag ccgttatcac aatgcaacga tcattcggaa aagggagcac
      121 gacgcaatga agcctgaatc gtcaaacggt gcgcaagcgc cctcgaagcc cctgacgaag
      181 ctcaaatcct tcgctgttcc ccaagcccgg ccgctgcctg tgatcgtgct cgccgatgtc
      241 agcgggagca tggccgagaa cgggaagatc gacgcactga acgtagccct tcgcgacatg
      301 gtcgcaagct ttgccaagga agggcggctt cgtgcagaga tccaagtggg cttgatcacg
      361 ttcggcggta agacggccaa ggagcacctg cctctggtcg ctgcgcattc gatcgcgatc
      421 atcgacaggt tcactgccga tggtggaacc ccgatggggg cggcgtttga tgttgcccgc
      481 gagttgctcg aagacaggga acgtatcccg tcgcgtgcct accggccggt cctggtattg
      541 gtgtccgacg gggctcctac agatgactgg gaagacgcac tggcccggct gcagagcagt
      601 gaacgcggca agaaggtaac ccggttcgcg atggcgatcg gagccgatgc tgacaaggac
      661 atgctcgcca acttcgccaa cgaccgcgag gcgccggttt tcgaggccca tgagtccagg
      721 gacatcatga ggttcttccg cgccgtcacc atgagtgttg tcgcgcgatc tgcaagcgcg
      781 acgccggatg aacctgccgc gctcacgttg agcgagattc ccgacgacga cctcgacatt
      841 ggtgccctgt agtgctcggg gcggccggag tgtccgtcac cgggcctggt catgtgtccc
      901 tggggcaagc gaaccaggat gcgatcgcac ttcgtggttg gcgcgggggg tggatcgccg
      961 ccgtagcaga tgggcttgga tcgcgtcccc ttagtgggtt gggctctcgg ctggccgtcc
     1021 aggccgcgca gcaaaccatc cgatcgatgc cgacagaaga gcccttgcgg gcatcggagc
     1081 cgcgacagac cgcaaccgag ttctatcggc ggtggcttcg ggcggtgccc tatgtcgaca
     1141 agtcctgcgt ggccagcacc atcctgatgg ccgcttgtgg tcgcgacggt atcggaaggg
     1201 tctggcagct tggcgacggg ctggttgtgt gccgctcgaa gggcagcttc agcgtggtca
     1261 cccctgaacg cttgggcttc ggcaatgaga cccgggccct agggattcac cgagcgtggt
     1321 cagactggtg caccgcagat gtggcgctac aggagcgtgg tgacgccgtg atcctcatga
     1381 ccgacggaat ctccgatgat gtccggattg aggcgctaga gatgttcact gatgcgctcg
     1441 tccgcggtgt catggcgagg actcgaaggg gcgcacgggg ttggctgcga aatgagttgg
     1501 tcaactggtc gacgccgaac cataccgacg acaagactgt ggcagtgatc ttcaagggat
     1561 aacgatgcag accgagactg gggcgggcga agcgaaggcg ggcggcaaga agcagcgcgt
     1621 gcaggacgag ttcggcggca tttacgagtt gaagagcaag ctcgcagagg gcggccaggg
     1681 catcgtactg agcacagact tccagaatct gctggtgaag gtaagccgct ggcccgatgg
     1741 agacccacgc acacaagcgt ggcgcaagca ggtcgaagca ctgcagcgca tgccggttgt
     1801 cgacgaaggg ctcccggtgg tcatgcccaa ggctcggatc gtgaagccaa gggcgggcta
     1861 cgtgatggaa cttgttgatg gcctgatccc ccttgagttg ctcctcaagg agtcgatcga
     1921 gtccttcgcc agcgggatgg ggcttcaggg ctttgtctca tccggcggtc tggcgcggcg
     1981 cctgcgagtc ctggcacgtc ttgcgcgcgt tctggcccaa ctccacgggc tcggcctcgc
     2041 gcacggtgac atctctccga agaacgtgtt cgtgtccaca tcgcatgacc agggcgaggt
     2101 ctggttgatc gactgcgaca acctgaccta tgccgtgcgc gacagttcgc tgcaggtcta
     2161 cacgccggac tacggtgccc ctgagatcct gcgcggagat gcgggcatca gcacctacac
     2221 cgatgtctgg agcttcgcgg tcatcgcatt ccaactcctt gttcaactgc accctttcaa
     2281 gggcggcgaa ctggttgacc aggattccga tcttgaggtc gcggcgctcc gaggtgagtt
     2341 gccctggatt gaccatcgtg acgatgctcg caaccggagc gcgatgggca tcgaccgcga
     2401 atacgtcctc accccgttgc ttgtcgaact gttcgaccgg tgctttcggc tgggcgtcgt
     2461 tgacccggaa ctgcggccca cgatgggtga gtgggcggag gcctttgagg ccgcgactgc
     2521 ggttcaggtc ctttgcctcg attccatcgg gtgcggaagt acgttctatt ggaacaaggc
     2581 catccagtgc cccttctgcg acacaaagca cgacccaggc tctgtgctgc gcctcggtca
     2641 cgcggtgtac gcgccgcctg cggagctcgg agaggatgcc gcgccggcag actcttggat
     2701 agccaccgac atagtcgcgg tactcagcgc caacgaactg gtgctaaggg ctgcgcctcc
     2761 gggaacttcc gcatatttcg atagcgccgt cgccgcagcg cttcagatcg atggggatga
     2821 actggtgatc cgagccggga agtcgaagcc acttcatctt caggtgtctg gcgcaaagag
     2881 tggtccggct ctgcggggaa cttcacgcat ccccagggat ggccgcgttc gtgcgctgca
     2941 cgtgggaccc cttgatgcaa cgcacgacgt ctggcggttc aagtggtgag gcagtatgtt
     3001 catccatgag tcgccgttgt tgcttccggg cctgcttgca gcgcagccgg aagacaagcc
     3061 aatcgatccg tcgccgtggc gggtaaacga agaagtgctc gtccgcgagg cggggggaga
     3121 actcctcctc cgccaggaca gccgcacgct ccggcttcgc ttgactccgc tgtcgggtcc
     3181 gctggaggga tacgcacagg atcagacatc catttgggtt ctcgcgcggg tgaagcccaa
     3241 cctgatctcc ctgaggtgcg cgcgtccttc acagagcaac tctttggaac ggccgctgga
     3301 gatcggtgtc gaccagcgcg tggccgacga tctccatcag cagagaaaga cctccgcgcc
     3361 gactgttgcg gcagccatgc agtggttgac cgatgaattt gtctgcgaga ggcaaggctt
     3421 tggcggtgac ctggttttcg cggcgatcct gccgcgtgcg aagagcggcc aactgcagtt
     3481 ggtcgggcgc gcgtggctcg tggacatcag gcgagccaac gacgggacgc ttctggtcga
     3541 gcgggttcgc cgacgcaatc agaagtccct ggacgcctat gcggtcctgg tgggagagat
     3601 ccgttttgtc gaccttgcgg ccgggatccg cgtccaggat ggtgacgagc gagccatgct
     3661 ggaggcatcg gtcagcagct atggcacgta cctggaactg tggcagctct acagcgacat
     3721 ggaatggcgc cgtgaggttc aaacggctgc ggaactgggc gcactaaggt acacacgttg
     3781 cgagccagca agcgctgagg gaggtgcctg gaggttcttt gctgatccgg cagagctcgg
     3841 cggcttccaa gcgcgatggc gctcacttgc ccagcaggac gacgcctcag tggaggctga
     3901 agaagcggcg ccggactggg gtgatgaccg ctacaccgac ctgtccacga gcgacactca
     3961 ccggcgcttt cgaggtgccc ccgagttccg tgaagggact ctgctcgcct tcgggcgcag
     4021 tcctatcgac cctccatctg ctgggtttat gttcctctcg ctcgggggag atcggaagca
     4081 gcacgaacgg cggcttcacg cacggcgtgc gatcgaggct gggatgggcg tgccaaggct
     4141 gcgcagcttg ctccaggacc taccaatccc tgggcagcgg ccatctcggt tgccagccct
     4201 gacgccctac gcaaggcaga gcttcaagtc agggaagccg acagcaaagc aagaactcgc
     4261 catccaggtc gcgctcaaca caccagatgt tgcgttgatc atcgggccgc ccggaacggg
     4321 aaagactcag gtcatcgcgg cgctggagag gcgcctggcg gagatcaacg aagggcaagt
     4381 catcgcgcat gaggttctga tctcaagctt ccagcatgac gcagtagaga acgcccttga
     4441 gcggaccagc gtctacggtc tacctgccat caaggttggc aatcgaagcg gtgcatccga
     4501 tgccgtagac cactggtgcg ccgctcggca tgagtccgtc agtcagcgat tggatcaact
     4561 ggcaatcgag gagccgcacg ttccgctgct gcagcgtctg aatcggatgg taatgggcct
     4621 cagactgacc agcatgccat cgcaccaacg cgaaggcgcc tttcagaagc tcaatgagct
     4681 gattgaggag ttggcgcaga aggctcgcct tcgcctgtca tctcgctggg tacaagactg
     4741 ggaagagtac ctggcgcgtc gtcactgcga cgatcttgat gttgtcagcc aactgcccgt
     4801 tggtcgccgc cgtcgcatta cacggctagc tcgatctctt cgagtcctat ccgagtcgtt
     4861 tgccgacgat ggtgcctttc gcgcaggttc actgctgagt gaagctggg
//
LOCUS       DAOORV010000236         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20689_length_4897_cov_7.299050, whole genome shotgun sequence.
ACCESSION   DAOORV010000236 DAOORV010000000
VERSION     DAOORV010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20689_length_4897_cov_7.299050"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKV56_18045"
     CDS             complement(<1..1268)
                     /locus_tag="PKV56_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX69754.1"
                     /translation="MQTSLRSLLAVGADNDPAISAPSRTTLTFSGLRELVDATLGSLN
                     SLGIGRNDRVAIVLDNGPEMATCFLACAAGVTSAPLNPGYRADEFEFYLSDLQARALI
                     VARGSASPAIAVASKLGVQIIDLVVAPAAPAGQFTLEPRAIQASTRVPDAGDAQPDDV
                     AMVLHTSGTTSRPKIVPLTVGNLCASAAHIRHTLQFMPADIGLNIMPLFHIHGLIAGV
                     LAPLSAGSQVFCTPGFNALKFFGWMEEAHPTWYTAVPTMHQAIAARGRGHGDSIRRHP
                     LRFIRSSSSSMPPQVIKEIEAVFDAPLIEAYGMTEATHQMASNPLPPKVRKPGTVGLP
                     GGPQVEIRGEDGRLLGPGEIGEIVIRGPNVTAGYENNPRANEEGYADGWFRTGDQGAK
                     DAEGYLSITGRLKEIINRGGEKVSPREVDE"
     gene            1426..2106
                     /gene="ccmA"
                     /locus_tag="PKV56_18050"
     CDS             1426..2106
                     /gene="ccmA"
                     /locus_tag="PKV56_18050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HOX69755.1"
                     /translation="MKSAPDPAVAPTPSAPEPVSARLELIGLSCVRGTRRLFSNVDSV
                     VQAGQLLRLQGANGAGKTSLLRTVCGLSAPDRGEVRWRGRSIAADRDAFHRDLIYLGH
                     AAALKDDLSALENMQAACMLGGQRPSPSQMMSALAVAGLRQREHLSARSLSQGQRRRV
                     SLARLVLKPSPPLWVLDEPFNALDVAATDWLLFVVTAQLRAGGIVVLTSHQPVAIDAS
                     LSQVTLTL"
     gene            2166..2822
                     /gene="ccmB"
                     /locus_tag="PKV56_18055"
     CDS             2166..2822
                     /gene="ccmB"
                     /locus_tag="PKV56_18055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOX69756.1"
                     /translation="MRSVFLRDLRLALRRRADTAAALFFFVIVASLFPLGVGPEPKLL
                     RTMAPGVVWVAALLASMLSLGRLFSDDHQDGTLEQLLLSVCPLPLIVLAKMAAHWLCS
                     GLALTLVAPVLALQFDLPAEAIGVLVVSLLLGTPLLSLIGGIGAALTVGVRGAGVLLS
                     LLVLPLCVPVLIFGAGAVDATLAGLPVGAHYSLLGAMLVLALFAAPFAAAAALRIALE
                     "
     gene            2906..3652
                     /gene="ccmC"
                     /locus_tag="PKV56_18060"
     CDS             2906..3652
                     /gene="ccmC"
                     /locus_tag="PKV56_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HOX69757.1"
                     /translation="MQFSEINWFKYAAPQRFYALVGRIVPWVSVLAVVLAAAGLVLGL
                     LIAPTDAQQGDAYRIIFIHVPAAWMSMVVYLAMAFWAAVGLIFNARLSAMLATALAPS
                     GALMTFIALWTGAFWGRPTWGAWWVWDARLTSELVLLFLYIGFMSLHAAIDDPRRADR
                     AAALLALVGAVNVPIIYFSVKWWNTLHQGASVSITKGSSMAAPMLAGMLVMALAFWAY
                     CIAVSLARLRVVILERERHAHWVQAELGNA"
     gene            3699..3878
                     /gene="ccmD"
                     /locus_tag="PKV56_18065"
     CDS             3699..3878
                     /gene="ccmD"
                     /locus_tag="PKV56_18065"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HOX69758.1"
                     /translation="MIWNSWTEFVAMGGYGLYVWGSFGVTAAVIAAEVWAVAARRRAL
                     IRLEHGARGADLGET"
     gene            3878..4366
                     /gene="ccmE"
                     /locus_tag="PKV56_18070"
     CDS             3878..4366
                     /gene="ccmE"
                     /locus_tag="PKV56_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOX69759.1"
                     /translation="MKPRTKRGLAIVVGLAALGIASTLVLNAFQSNLVFFFSPSQVAA
                     NEAPRERSFRVGGLVEEGSIVRDSKTLQVNFVVTDLAKTVPVVYTGLLPDLFREGKGV
                     VAQGKMGADGVFHAEQVLAKHDENYMPPEAADALAKARKGDLPAGAMSSSSEMRSPGA
                     QP"
     gene            4363..>4897
                     /gene="ccsA"
                     /locus_tag="PKV56_18075"
     CDS             4363..>4897
                     /gene="ccsA"
                     /locus_tag="PKV56_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOX69760.1"
                     /translation="MIPEIGHVALILALTMALAQSVLPLVGAQQGNAAWMSLARPSAR
                     GQFIFMLIAYGCLTYAFVNNDFSVLLAAEHSNSSLPLMYRLTAVWGNHEGSILLWSLI
                     LSGWTFAVSIFSKRLDDVTVARVLGVMGLVSCGFMLFTLFTSNPFERLLPAAPDGRDL
                     NPLLQDPGMVIHPPMLYM"
BASE COUNT          703 a         1606 c         1655 g          933 t
ORIGIN      
        1 atctcgtcga cctcgcgcgg actgaccttc tcgccgcccc tgttgatgat ctccttcagg
       61 cggcccgtga tgctgaggta gccctcggcg tctttcgcgc cctgatcgcc cgtgcgaaac
      121 cagccgtcgg cgtagccctc ttcgttggcg cgcggattgt tctcgtagcc agcggtcacg
      181 ttcgggccgc ggatcacgat ctctccgatc tcacccggcc ccagcagcct gccgtcctcg
      241 ccgcggatct ccacctgcgg accgccggga aggcccacgg ttccgggctt gcgtaccttc
      301 ggaggcaagg gattcgaggc catctggtgc gtggcttcgg tcatgccgta ggcctcgatc
      361 aacggcgcat cgaagacggc ttcgatctcc ttgatgacct gcggcggcat cgacgacgac
      421 gacgacctga tgaatcgcag cggatggcgc cggatggagt cgccatgccc cctgccccgc
      481 gcagcaatcg cctggtgcat ggtcggcacg gcggtgtacc aggtgggatg agcctcttcc
      541 atccagccga agaacttcag cgcgttgaag cccggcgtgc aaaacacctg cgatcccgcc
      601 gacagcggtg ccagcacccc ggcgatcagc ccgtggatgt ggaacaacgg catgatgttc
      661 aggccgatgt cggccggcat gaactgcaag gtatggcgga tgtgtgctgc cgatgcgcac
      721 aggttgccca ccgtcagcgg aacgatcttc ggccgcgaag tggtccccga ggtgtgcagc
      781 accatcgcga cgtcatcggg ctgcgcgtca cccgcgtcgg gcacgcgcgt gctggcctgg
      841 atagctcgcg gctcgagggt gaactggccg gctggcgcag ccggcgcaac caccagatcg
      901 atgatctgca cgccgagctt cgaagccacg gcgatcgcag gcgacgcgct gccccgcgcg
      961 acgatcaggg cgcgcgcctg caggtcgctc aggtagaact cgaactcgtc ggcgcggtag
     1021 cccggattca gtggggcgct ggtgacgccc gccgcacagg ccagaaagca ggttgccatc
     1081 tcggggccgt tatcgagcac gattgcaacg cggtcgttgc gaccgatgcc cagcgagttc
     1141 aggctgccca gcgtggcgtc cacgagttcg cgcagcccgg agaacgtcaa ggtcgtacgc
     1201 gacggggccg aaatcgccgg atcgttgtct gcgccaacgg caaggaggga tcgaagtgaa
     1261 gtctgcatgg cggtgccgag gtctgaggct ctgtgcgaac cacgggtgat gtgactcagt
     1321 ttggaccgac tttagcggcg ccgggataat gccggttgcg tcgggctcgc gaacagcgcg
     1381 agtccgacag ccccttactt gaatgcgatc ctgcatcccc ctgccttgaa gtctgccccc
     1441 gaccccgcgg tcgcgcccac tccttctgcg ccggaacccg tttcggcccg actcgaattg
     1501 atcggactct cgtgcgttcg ggggacgcgc cgcctgttct cgaacgtcga ctcggtcgtg
     1561 caggccggac aactgcttcg cctgcagggc gcgaatggtg cgggcaagac cagcctgctg
     1621 cggacggtgt gcggactgtc tgctccggac cggggtgaag tgcgctggcg cggtcgctcc
     1681 atcgcagccg atcgagacgc ctttcatcgg gatctgatct acctgggaca cgctgcggcg
     1741 ctgaaggatg acctgtctgc cctcgagaac atgcaggctg cctgcatgct gggagggcag
     1801 cgaccgtctc catcccagat gatgtccgca ctcgccgtgg ccggactgcg acagcgcgag
     1861 cacctcagcg cccgcagcct gtcgcagggc cagcgccggc gggtctccct cgcgcggctg
     1921 gtgctgaagc cgtcgccccc gctgtgggtg ctcgacgagc ccttcaacgc gctcgacgtg
     1981 gcagccaccg actggctgct tttcgtcgtg accgctcagt tgcgcgcagg cggaatcgtg
     2041 gtgctgacca gccaccagcc ggtggcgatc gatgcatcgc tctcccaggt cacgttgacg
     2101 ctgtgacgcc cgcaccgacc ccagcgaact ggcagcacac cgtgccgtcc agcgccctcg
     2161 acgccatgcg cagcgttttc ctgcgcgacc tgcgtcttgc gttgcgccgc cgcgccgaca
     2221 cggcggcagc gctgttcttc ttcgtgatcg tcgccagcct gttcccgctc ggcgtcggtc
     2281 ccgagccgaa actgctgcgc acgatggcgc ctggcgtggt ctgggttgcg gcgctgctgg
     2341 cgtccatgct gtcgctggga cgcctgtttt cggacgacca ccaggacggc acgctggagc
     2401 aactgctcct ttcagtttgt ccgctgccgc tgatcgtgct ggcgaagatg gcggcgcact
     2461 ggttgtgttc cgggctggcg ctgacgctgg tggcgcccgt gctggccctg cagttcgacc
     2521 tgccggccga ggcgatcggc gtgctggtgg tgtcgctgct gctgggaact cccctgctga
     2581 gcctgatcgg cggcatcggt gccgcgctga cggtgggcgt ccgcggcgcc ggggtgctgt
     2641 tgtcgctgct ggtgctgccg ttgtgcgtgc cggtgctgat cttcggcgct ggcgcggtcg
     2701 atgccaccct ggccggcctg cccgtgggtg cgcattactc cctgctgggt gcgatgctgg
     2761 tgctggcgct gtttgccgcc cccttcgccg ccgccgccgc cctgcggatc gcgctcgaat
     2821 gagtgcgttg cacaataacg ggctgtctgg agcgccggtc tgttgccggc ctgcgtccaa
     2881 agggtgacag ccggagcccg aattcgtgca gttctctgaa atcaactggt tcaagtacgc
     2941 cgctccgcag cgtttctatg cgctcgtcgg gcgcatcgtt ccctgggtga gcgtgctcgc
     3001 ggtggtgctc gccgcagccg gccttgtcct cggcttgctg attgcaccga ccgatgcgca
     3061 gcagggcgac gcctatcgca tcatcttcat tcacgtgcct gccgcgtgga tgtcgatggt
     3121 ggtctacctg gcgatggcgt tctgggccgc cgtcgggctg atcttcaatg cacggctgtc
     3181 cgcgatgctg gccacggcgc tggcgccgtc gggcgcactg atgaccttca tcgcactgtg
     3241 gaccggcgcc ttctggggtc gtccgacctg gggcgcatgg tgggtctggg acgcacggct
     3301 cacgtcggag ctggtgctgc tgttcctcta catcggcttc atgtcgctgc acgccgccat
     3361 cgatgatccg cgacgagccg accgcgctgc ggcgctgctg gcactggtcg gtgcagtcaa
     3421 cgtcccgatc atctacttct ccgtgaaatg gtggaacacg ctgcaccagg gtgcttccgt
     3481 cagcatcacc aagggctcga gcatggcggc accgatgctg gcgggcatgc tcgtgatggc
     3541 actggccttc tgggcctatt gcatcgcggt gtccctggcg cgactgcggg tcgtcatcct
     3601 cgagcgcgaa cggcatgcgc actgggtgca agctgaattg gggaacgcat gagcggtccg
     3661 gcgagtccat gggacctgca aagtctgaag gactgcggat gatctggaac agctggaccg
     3721 agtttgtcgc gatgggcggc tacggactct acgtgtgggg ctcgttcggt gtcacggcag
     3781 ccgtgatcgc cgccgaggtc tgggcggtcg ccgcgcgccg ccgggcactg atccgacttg
     3841 aacacggcgc ccgcggcgcc gatctgggag agacctgatg aaaccgagaa ccaagcgagg
     3901 cctggccatc gtggtgggcc tggcagccct cggcatcgcc agcacgctgg tgctcaacgc
     3961 gttccagagc aatctggtgt tcttcttcag cccgtcgcag gtggcggcca acgaggcccc
     4021 gcgcgagcgc agcttccgtg tcggcgggtt ggtcgaagag ggaagcatcg tgcgcgattc
     4081 gaagaccctg caggtcaact tcgtggtgac cgatctggcg aagacggtcc cggtcgtcta
     4141 caccggcctg ctgcccgacc tgttccgtga gggcaagggt gtcgtggcgc agggcaagat
     4201 gggagcggac ggcgtcttcc atgccgagca ggtgcttgcc aagcacgacg agaactacat
     4261 gccacccgaa gcggccgacg cgctggcaaa ggcacgcaag ggcgatctgc ctgcgggggc
     4321 catgagttcc tcgtccgaga tgcgcagtcc tggagcccag ccgtgattcc cgagatcggt
     4381 catgtggcgc tgatcctggc gctcacgatg gcactcgccc agagcgtgct gccgctggtc
     4441 ggcgcacagc aagggaacgc cgcctggatg tcactggcgc gcccctcggc gcgtggccag
     4501 ttcatcttca tgctgatcgc ctacgggtgc ctgacgtatg ccttcgtcaa caacgatttc
     4561 tcggtgctgc tggccgccga acattcgaat tcgtccctgc cgctgatgta tcgcctcacg
     4621 gcggtttggg gcaaccacga gggctcgatc ctgctgtggt ccctgatcct gtccggctgg
     4681 acgttcgcgg tgtctatctt ctcgaagcgg ctggacgacg tgacggtcgc acgcgtgctc
     4741 ggcgtgatgg ggctcgtgag ttgcggcttc atgctgttca cgctgttcac gtcgaatccc
     4801 ttcgagcgac tgttgccggc cgctcctgac ggccgcgacc tgaacccgct gctgcaggac
     4861 ccgggcatgg tgatccaccc gccgatgctc tacatgg
//
LOCUS       DAOORV010000237         4883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20746_length_4883_cov_8.119304, whole genome shotgun sequence.
ACCESSION   DAOORV010000237 DAOORV010000000
VERSION     DAOORV010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20746_length_4883_cov_8.119304"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PKV56_18080"
     CDS             <1..772
                     /locus_tag="PKV56_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX69761.1"
                     /translation="QALFFGFGAYVSALVLKHVAPSLPLAMFIVLVSSTVVGLLGGVI
                     AIRAKGVYFALISFGLAQVVSKVVFNTRELGASDGLIGVPAPVWLGLDTAHAPSFFLL
                     VLLGMAALLAFLLWLMNTPFGRQLDAIRTNEHRLPFLGFDPWRYKLAVFVLAAQVAAL
                     SGALYPMLRGFVSPELMFFQVSGNAVINVIVGGTGTLVGALYGSALLTVLKSVVGSFT
                     AHHTIVIGALFVVSVIFFPKGLVGYLAPALQRWRSKRA"
     gene            769..1536
                     /locus_tag="PKV56_18085"
     CDS             769..1536
                     /locus_tag="PKV56_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX69762.1"
                     /translation="MNGDTILNVRGLTVRFGTLVAVSDVSFDAQRGHITSVIGPNGAG
                     KSSLFNLISGAIRPSAGQVLFEGRDMTGQRPDKMLAAGLARSFQITNLFFELPVRENL
                     RLAAQFVENGRGLFRGIAASRVALARVDELIERFALQTRADELAGFLSHGEQRRLEIA
                     VALAARPRLLLLDEPTQGMSHADTQDTEALIRSLAAEGLSLLLVEHDVELVMNLSDHV
                     VVMHQGAKLAEGPPLQVRADPAVQAAYFGDAREASHA"
     gene            1529..>1875
                     /locus_tag="PKV56_18090"
     CDS             1529..>1875
                     /locus_tag="PKV56_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX69763.1"
                     /translation="MLELKDVHTHYGLSHVLQGVSLRVDAGEVVGLFGRNGVGKTTVM
                     KTIAGWVAPTQGTVHFDGQAIAGLASERICRQGMGFVPEDRRIFPGLTVEENLVLGFM
                     QSPGRSRAEDRRQL"
     assembly_gap    1876..1975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1976..2391
                     /locus_tag="PKV56_18095"
     CDS             <1976..2391
                     /locus_tag="PKV56_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX69764.1"
                     /translation="LGFMQSPGRSRAEDRRQLDAIYTRFPRLGERRQQMGTTLSGGEQ
                     QMLAMARVLVGQPRLLLIDEPTEGLAPKIVDEIFTLMEGLRRDGIPILLVEQNMHRAI
                     GLVQRFYVLERGAVVMSGDGTKAADREALMRQLAV"
     gene            2393..2800
                     /locus_tag="PKV56_18100"
     CDS             2393..2800
                     /locus_tag="PKV56_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69765.1"
                     /translation="MAVTDRRIAWLLTAEAFECGSAAIKAGLSVASEAGFRVVAMNYS
                     MANGEISPEIEAEAMRLGMQSILVNDAAHCRLGSGLRDAERWSLAQALSRDGFDGFIL
                     CEVCDSRTRAESLLATARVLLRNLDQLDWESYH"
     gene            complement(2880..3131)
                     /locus_tag="PKV56_18105"
     CDS             complement(2880..3131)
                     /locus_tag="PKV56_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69766.1"
                     /translation="MTDEASGDIHESYIMLTINADAHPLFAGMHMPDSNLVPNRRGNR
                     SVITVSREGVYPWLYWTIEGALNLLRLKPAEHFDAVARR"
     gene            complement(3156..3383)
                     /locus_tag="PKV56_18110"
     CDS             complement(3156..3383)
                     /locus_tag="PKV56_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOX69767.1"
                     /translation="MVVGQPGLIPWFAEMPTLTYSTSNARSEDMSQKPTYKDPWRYGK
                     RGIIPALSFEGPCWETGRNVWWRLWRADGKP"
     gene            complement(3632..4255)
                     /locus_tag="PKV56_18115"
                     /pseudo
     CDS             complement(3632..4255)
                     /locus_tag="PKV56_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052404.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4600..4863)
                     /locus_tag="PKV56_18120"
     CDS             complement(4600..4863)
                     /locus_tag="PKV56_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOX69768.1"
                     /translation="MLAFQVNDMTCGHCAGAITKAVKYLDQAAKVTIDLAQHLVTVEP
                     TAADGEQIRDAIAGAGYTPEPVVRPVPATAGTATTRTCCGSCH"
BASE COUNT          768 a         1516 c         1627 g          872 t
ORIGIN      
        1 gcaggcgctg ttcttcggct tcggggcgta cgtctcggcg ctggtgctca agcacgtggc
       61 gccttcgctg ccgctggcga tgttcatcgt gctcgtcagc agcaccgtgg tgggcttgct
      121 cggcggtgtc atcgccatcc gcgccaaggg cgtgtacttc gcactcatca gcttcggcct
      181 ggcccaggtg gtcagcaagg tggtgttcaa cacgcgcgag ctcggtgcgt ctgacggcct
      241 catcggcgtg cccgcgccgg tgtggctggg gctggacacc gcgcacgcac ccagcttctt
      301 cctgctggtt ctgctgggca tggccgcgct gctggccttc ctgctgtggc tgatgaacac
      361 acccttcggc cgccagctcg acgccatacg caccaacgag caccggctgc ccttcctggg
      421 cttcgacccc tggcgctaca agctcgcggt gttcgtgctg gcagcccagg tggcagcgct
      481 cagcggtgcg ctgtacccca tgctgcgcgg cttcgtcagc cccgagctga tgttcttcca
      541 ggtctcgggc aacgcggtca tcaacgtcat cgtcggcggc accggcacgc tggtgggtgc
      601 gctctacggc agtgcgctgc tgacggtgct gaagtcggtg gtgggcagtt tcacggcgca
      661 ccacaccatc gtcatcggcg cgctcttcgt cgtctcggtg atcttctttc ccaaggggct
      721 ggtgggctat ctggcgccgg cgctgcaacg ctggcggagc aaacgcgcat gaacggcgac
      781 accatcttga acgttcgtgg cctgacggtg cgcttcggca cgctggtggc cgtcagcgac
      841 gtcagcttcg acgcgcagcg cggccacatc acctcggtga tcggccccaa cggtgcgggc
      901 aagagcagcc tgttcaacct gatcagcggc gccatccggc ccagtgccgg gcaggtgctg
      961 ttcgaaggcc gcgacatgac cggccagcgg ccggacaaga tgctggctgc cgggctggcg
     1021 cgttcgttcc agatcaccaa cctgttcttc gaactgccgg tgcgcgagaa cctgcgtctg
     1081 gccgcgcagt ttgtcgagaa cggccgcggc ctgttccgcg gcatcgccgc cagccgcgtg
     1141 gcgctggcgc gggtggacga gctgatcgaa cgctttgcgc tgcagacgcg ggcggacgag
     1201 ctggcgggtt tcctgtcgca tggcgagcag cgccggctcg agatcgccgt ggcgttggcc
     1261 gcgcgaccgc gcctgctgct gctggacgag ccgacgcaag gcatgagcca tgccgacacc
     1321 caggacaccg aggcgctgat ccgcagcctg gctgccgagg gcttgagcct gctgctggtg
     1381 gaacacgacg tcgaactggt gatgaacctg tcggaccatg tggtggtgat gcaccagggc
     1441 gccaagcttg ccgagggccc gccgctgcag gtgcgtgccg accccgcggt gcaggccgcc
     1501 tacttcggcg atgcgcgcga ggccagccat gcttgaactg aaggacgtgc acacgcacta
     1561 cggcctgagc catgtgctgc agggtgtgag cctgcgcgtg gatgccggcg aagtggtggg
     1621 cctgttcggc cgcaacggcg tcggcaagac gacggtgatg aagaccatcg ccggctgggt
     1681 ggcgccgacg cagggcacgg tgcacttcga cggccaggcc atcgccggct tggcctcgga
     1741 acgcatctgc cgccagggca tgggcttcgt gcccgaggac cggcgcatct ttccgggcct
     1801 gacggtcgag gagaacctgg tgctgggctt catgcagagc cccggccgca gccgcgccga
     1861 agaccggcgc cagctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgctg
     1981 ggcttcatgc agagccccgg ccgcagccgc gccgaagacc ggcgccagct cgacgcgatc
     2041 tacacccgct tcccgcgcct gggtgaacgc cgccagcaga tgggcaccac gctgtcgggc
     2101 ggtgaacagc agatgctggc gatggcacgc gtgctggtgg gccagccgcg cctgctgcta
     2161 atcgacgaac ccaccgaagg gctggcgccg aagatcgtcg acgagatctt cacgctgatg
     2221 gaaggcctgc gccgggacgg catcccgatc ctgctggtcg aacagaacat gcaccgtgcg
     2281 atcggtctgg tgcagcgctt ttacgtgctg gagcgtggcg cggtggtgat gtcgggcgac
     2341 ggcacgaagg ccgccgatcg cgaggcgttg atgcggcaac tggcggtctg acgtggcggt
     2401 aaccgaccga cgtattgcct ggctgctgac ggccgaagcg ttcgagtgcg gcagtgccgc
     2461 gataaaggcg ggcctgtccg tggcttcgga ggcaggattt cgtgtggtgg cgatgaacta
     2521 ctcgatggca aatggcgaaa tcagtccgga gatcgaggcg gaagcgatgc ggctcggtat
     2581 gcagtccatc ctcgtcaacg atgcggcgca ctgccgtctc ggttcgggac tgcgcgatgc
     2641 ggagcgctgg tcgctggcgc aggctctctc gcgtgacggt tttgacgggt tcatcctgtg
     2701 tgaagtctgc gatagccgta ccagggctga atcgctgctc gcgacggctc gtgtgctgtt
     2761 gcggaatctg gatcaactcg actgggagtc ttaccactag gatcgaaccg agtcattcgt
     2821 tgaacgggag gtcggagctc aggcgaagcc tgttgcccat gttgtcgccc tgacccccat
     2881 cagcggcggg ccacggcgtc gaagtgttcg gcgggcttga ggcgcagcag attcagggca
     2941 ccctcaatcg tccagtagag ccaggggtaa acgccctctc ttgagacggt gataacgctg
     3001 cgattgcccc gccggtttgg cacgagattc gagtcgggca tgtgcatgcc tgcaaacagc
     3061 ggatgcgcat cggcgttgat ggtcagcatg atgtacgact cgtggatgtc gccgctggcc
     3121 tcgtcggtca aggtgttcca cagaccggcg aggccctatg gcttgccgtc ggcccgccag
     3181 aggcgccacc agacgttcct tccggtctcc cagcacggtc cttcgaagga gagggccggg
     3241 atgatgccgc gcttgccgta acgccaggga tccttgtagg tcggcttctg gctcatgtct
     3301 tcactgcgcg cgttgctcgt cgaatacgtc agcgtcggca tctcggcgaa ccaggggatc
     3361 aggcccggct ggccaaccac caattcggcg gccggttctg tcgcatcccg catgcaacgg
     3421 acttgcggct ccgggcggcg agggcgccat tgaatggctc cctcatagcc cgcggctcga
     3481 gcatgcccct gtgggactcc agaaagccgc ccttcgccta cgacggcaaa tggccgatac
     3541 cgtgagttcg ctgcgcctca aggaaactgc cgctcagggt aggagacggc gccgcgactg
     3601 cccagccgaa gcgccgcctg gatcggccac ctcagatctc ggtttgctcc gagatctcca
     3661 gggcatcgtc gacctcgata cccaggtagc ggacggtcga ttccagcttg gaatggccaa
     3721 gtagcagttg aactgcaagc aggttcttgg tgcgtcggta gatcagcgtt gccttggtcc
     3781 gccgcatcga gtgggtgccg tagtcggcgg gatccaagcc caggtcgtgc acctagccgc
     3841 caagaatccg agcgtactgt cgcgtgccga gatgcgggga ctcatggatc cggcttggga
     3901 acaggaagtc atccgacttc agctccgcta gcttgatcca ggcctggacg gcgtcgcgcg
     3961 tcgccgacgt gatctcgaac tggaccgggc gctgcgtctt gtgttgcatc acggtggcgc
     4021 gagtggcaac ctggtcgcca tgacagatgt cgcgcacctt gagcgcgacc agatcgcagc
     4081 cgcgaagctt gctgtcgatg cccaggttga acagggccag ttcccggaca cggccctcca
     4141 tctggaggcg gatgcggagc gcccagatgt ccttgagctt gaacggcgcc ttctgcccaa
     4201 cgatcttgcc cttgttccac ggctcgcggt gaacggcgct gacattggat tccatgacgg
     4261 tctccagtcg ggttgagggg cgaagacgat gacccttcgc catcccgaac accttgcccg
     4321 acatcaacca gtctgccgtc tctaccgacg ggggcgaaac gtgaagggca gattcctgga
     4381 gacgccgcga actcacggac ccggccacaa gcggtcggtc gtctcgctgc caaatagccg
     4441 acggtcgacc agcggatgcc acgtctctat ggcatcggcg tagccgccga cgtcctggga
     4501 agtgttgttc tcagcgcatc gtttggtcgc agcaaaattc atggccgagt cggcgccgaa
     4561 caccagccat ctcggcccct catcaagggc gacgcgagct cagtggcacg agccgcaaca
     4621 cgtgcgagta gtcgcggtgc ctgcagttgc cggaaccggt cgcacgaccg gctccggcgt
     4681 gtacccggca ccggcaatgg cgtcgcgaat ctgctcgcca tcagcggcgg tcggctcgac
     4741 ggtcaccagg tgctgggcga ggtcgatcgt gaccttcgca gcctgatcga ggtacttcac
     4801 tgccttggtg atggcgccgg cacagtgacc gcaggtcatg tcgttgactt gaaaggcaag
     4861 catggggcgt tccttggaac tgg
//
LOCUS       DAOORV010000238         4871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20801_length_4871_cov_8.732350, whole genome shotgun sequence.
ACCESSION   DAOORV010000238 DAOORV010000000
VERSION     DAOORV010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20801_length_4871_cov_8.732350"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKV56_18125"
     CDS             complement(<1..1036)
                     /locus_tag="PKV56_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOX69769.1"
                     /translation="MSETTHLNSDALGSWQKAAAKSAPGGQVDALNWHTPEGLVVKPL
                     YTAADLKGLPNTDTLPGFPPYLRGPQATMYAVRPWTIRQYAGFSTAEASNAFYKKALA
                     AGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDAIPLDKVSVSM
                     TMNGAVLPVLAGYVVAAEEQGVAQDQLSGTIQNDILKEFMVRNTYIYPPEPSMRVIGD
                     IIEYTAKKMPKFNSISISGYHMQEAGANQALELAFTLADGKEYVKTALAKGLDVDEFA
                     GRLSFFWAIGMNFYLEVAKMRAARLLWWKIMKEFAPKNPKSLMLRTHCQTSGWSLTEQ
                     DPYNNVVRTTI"
     gene            1218..1937
                     /locus_tag="PKV56_18130"
     CDS             1218..1937
                     /locus_tag="PKV56_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69770.1"
                     /translation="MSPLDAPDTPKPAAAARPPAAGGEELPDRLRLPSTALYQQVAER
                     LRSRIMSHTLAPGSWIDEQALAAEYGTSRTPLREALKVLAAEGLVTMKLRRGAYVTEV
                     SERDLTEVFHLLSLLECDAVGVVATRATDAQLADLAALHDRLEAAANDAEAFFTANER
                     FHMRLLEIADNRWRNQMVADLRQVMKLNRHHSLLKEGRLTASLQEHRAIMAALTARDA
                     NGAQQLMRQHMAQGQEAAALS"
     gene            complement(2107..2523)
                     /locus_tag="PKV56_18135"
     CDS             complement(2107..2523)
                     /locus_tag="PKV56_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOX69771.1"
                     /translation="MERSTRQRTAISAVIDSAGRPLSTQEVLDAAQVDVPGLGLATVY
                     RNLKLMVDSGEIAAVTLPGDSPRYESTQHAHEHHHHFQCTSCKRVFDVHDCPGDLARL
                     APKGFTVERHDLTLYGRCDACLGPRKTLRRAGSARP"
     gene            3153..4094
                     /locus_tag="PKV56_18140"
     CDS             3153..4094
                     /locus_tag="PKV56_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOX69772.1"
                     /translation="MSRTHLSALHARGLVTTRRKLLFSSAALLAPGRTLAASGRNSSP
                     LKVVASFSILADLLRNVGGDLIEISTLVGPNADAHVFEPSPADALRLAHADLVVVNGL
                     GFEGWLDRLVQASGYRGPVVIASIGVDVRTSGSTPDPHAWQDLSHARRYVRNVRDALV
                     RARPSEARQFHERAARYTDRLSALDAKVRLSFDSIPKGARRVITSHDAFGYFGAAYGI
                     DFLAPHGMSTQGEASAASVARLVKQIRQEGVRALFVENITDSRLIEQIAREGGAVVGG
                     RLYSDALSPPGTEADTYLKLFAHNASAIAASLKGRSP"
     gene            4091..>4871
                     /locus_tag="PKV56_18145"
     CDS             4091..>4871
                     /locus_tag="PKV56_18145"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX69773.1"
                     /translation="MSLIPVTIVTGLPCSGKSTLIQRLIQHQRDERIVWIAKSFEATT
                     IPFDIDLWVLSVEEQAVEIDNGDMRCVVRSDLLHILQTLQSQRSAGVLRFDRVIIETS
                     GFAEPAPVVQTFFAEESICDQYRLDAVVTLLDAEHAMAQLDAHREAQDQVGFADRIVL
                     TRTDRIAVDASRALHRRLTRMNPRAQVLSREGDLEMAQQLLDIRGFALTGVLDIDADG
                     LVDAKNARDGDVTSFVFRTRQSFDKQRVEDFFSSMLLVYGDR"
BASE COUNT          880 a         1594 c         1487 g          910 t
ORIGIN      
        1 cgatggtggt gcgcaccacg ttgttgtacg ggtcctgctc ggtcaggctc cagcccgagg
       61 tctggcagtg ggtgcgcagc atcaggctct tcgggttctt gggcgcgaac tccttcatga
      121 tcttccacca cagcagccgc gccgcgcgca tcttggccac ttccagatag aagttcatgc
      181 cgatggccca gaagaagctc aggcgcccgg cgaactcgtc gacgtccagc cccttggcca
      241 gcgccgtctt cacgtactcc ttgccgtcgg ccagcgtgaa ggcgagttcc agcgcctggt
      301 tcgcccccgc ctcctgcatg tgatagccgg aaatcgagat cgagttgaac ttcggcatct
      361 tctttgccgt gtactcgatg atgtcgccga tgacccgcat gctcggctcg ggcgggtaga
      421 tgtaggtgtt gcggaccatg aactccttga ggatgtcgtt ctggatggtc ccgctcaact
      481 gatcctgcgc aaccccctgc tcctcggccg ccacgacata gcccgccagc accggcagca
      541 ccgcgccgtt catcgtcatg ctcacgctga ccttgtcaag cgggatggcg tcgaacagga
      601 tcttcatgtc ctcgacgctg tcgatcgcca cgcccgcctt gcccacgtcg cccgtgacgc
      661 gcggatggtc gctgtcgtag ccgcggtgcg tcgccaggtc gaatgcaacg ctgacgccct
      721 gcccgcctgc cgccagggcc ttcttgtaga aggcgttcga tgcctctgct gtcgagaagc
      781 cggcgtactg gcggatcgtc cacgggcgca ccgcgtacat cgtggcctga ggcccgcgca
      841 gatagggcgg gaaacccggc agggtgtcgg tgttcggcaa gcccttcaga tctgccgctg
      901 tatagagcgg cttgaccacc aggccttccg gcgtgtgcca gttcagggca tccacctgcc
      961 cgcccggggc agacttggca gcggctttct gccagcttcc cagcgcatcg gagttgaggt
     1021 gcgtggtttc agacatgggg ggctccgttt ggcttggcat gacgcatcag cgacgcctcg
     1081 gaactgtaac ttttccggcg tcacatcatg cataattcaa aactgaatta taaactgaag
     1141 ttcagtttct cttcgcgttt tcccggggcg agtgctgtac gctcccgccg cgtgaatcaa
     1201 cccacaggca tctgcgcatg agcccgctcg acgctccgga tacaccgaaa cctgctgcag
     1261 cggcacgccc gcccgctgcc ggcggcgaag agttgccgga ccgactgaga ctgccgtcga
     1321 ccgcgctgta ccagcaagtg gcggagcgtc tgcgctcgcg gatcatgtcg cacacgctgg
     1381 ccccgggaag ctggatcgac gaacaggccc tggccgccga gtacggcacc agccgcaccc
     1441 cgctgcgcga ggcactgaag gtgctcgcgg ccgaagggct ggtgacgatg aagctgcggc
     1501 gcggcgccta cgtcaccgag gtgtccgaac gagacctgac cgaggtgttc cacctgctct
     1561 cgttgctcga gtgcgatgcg gtcggcgtcg tggccacgcg tgcgaccgac gcgcagctgg
     1621 ccgacctcgc ggcattgcac gaccggctgg aggcagcggc caacgatgcg gaagcgttct
     1681 tcactgccaa cgaacgcttt cacatgcgcc tgctcgagat cgccgacaac cgctggcgca
     1741 accagatggt ggccgacctg cgccaggtga tgaagctgaa ccgccaccac tcgctgctca
     1801 aggagggccg gctcacagcc tccctgcagg aacatcgggc catcatggct gcgctgacgg
     1861 cacgcgatgc gaacggcgcg cagcaactga tgcgtcagca catggcgcag ggtcaggaag
     1921 ccgcggctct gtcctgatcc gcaactccgg gacgcccgcc actctcaatc gcgccggatt
     1981 cgccgaacga agcgcgccat cttcgattca agggattccc cggcaaaacg acgtgatgcg
     2041 ccgaacccag taactctggg aaatcgagcc aggtgcgatc gcaagagcgc catcgcctgt
     2101 ccccggtcag ggccgggcgc taccggcgcg acgcaacgtc ttgcgcgggc cgaggcacgc
     2161 gtcacatcgg ccgtaaagcg tcagatcgtg acgctcgacg gtgaagccct tcggtgcgag
     2221 cctcgccaga tcgcctggac agtcatgcac atcgaacaca cgcttgcaag acgtgcactg
     2281 gaaatgatgg tgatgctcgt gtgcgtgttg agttgactcg tagcgtgggc tgtcccctgg
     2341 cagggttacg gctgcgatct ctcccgaatc caccatcagc ttcaagttgc gatagacggt
     2401 cgccagaccg agtcctggca cgtcaacttg cgctgcatcc agcacctcct gggttgaaag
     2461 cggtcggccg gccgaatcga tcacggcgct gatggcggtg cgttggcgag tagagcgttc
     2521 cattgattca gcagtccatt tggcgatgac ttccaaagtc tcgcatgcta tgcttttgat
     2581 atcgcgttct cgttatcact acggcggaaa tcccacgcac aaaggtcacg acttccttca
     2641 gaaccttccc ggctggcttt ctgcaacgcc gctgagcgaa agctggagtc cccaaagcaa
     2701 accatgctgt cagcttcgaa ttcagtgcga actcgtatca gtcacgcctc ccgtgccgtg
     2761 cctcgcgatc gggggcagtt gcttggttca agtgtcgata ctcaatggcc ctgtacaggt
     2821 ttacttccgg gaccgagact gcccgccttg tggccccgag ctgcaacgcc tcgccgaatt
     2881 tccccggacc cgacaccggt gggaagtgct gcaaatcggc acggacgcac tcatcgctgc
     2941 ccgcgagttc gcacgatctc gcggcatgac gctcaacgtg ctgaggctgg cgccaacgta
     3001 gccgcactga tgcgcctggc gggcaaccgc cacggcggcc tgccgtgctc ggtcgcactg
     3061 cagcaatcaa gcacctgcga tcgacaactc ggcgaggtgt cgccgaacga ccttctgcgc
     3121 atcggtacgg catgcacagc gggatcgtcg ccatgagtcg cacccatctc agcgcgctcc
     3181 atgcccgcgg actcgtcacg acgcggcgca agttgttgtt ctcctcagcc gcactgttgg
     3241 cgccaggccg cacgctcgcc gcgtctggtc gaaactcaag cccactgaaa gtcgttgcca
     3301 gcttctcgat cctggccgac ttgctgcgca atgtgggcgg cgatctgatc gagatcagca
     3361 cgctggtcgg cccgaatgcc gacgcgcatg tgttcgagcc cagcccagcc gatgccttgc
     3421 gtctggccca tgcggatctg gtggtcgtca acgggctggg cttcgagggc tggctggacc
     3481 gcctggtgca agcctcgggc taccgtggtc cggtggtcat cgccagcatt ggcgtggatg
     3541 tgcgcacctc aggctcgaca cctgatccgc atgcatggca ggacctgagc cacgctcgcc
     3601 gctacgtgcg caacgtgcgc gatgcactgg tgcgggcgcg gccctctgaa gcccgtcaat
     3661 tccacgagcg agcggcccgt tacaccgatc gcctgagcgc gctcgacgcc aaagtacgcc
     3721 tgtccttcga cagcattccg aaaggcgcgc gccgagtcat cacatcacac gatgcgttcg
     3781 gctactttgg cgccgcgtac ggaatcgact tcttggcgcc gcacggcatg agcacgcaag
     3841 gcgaagcgtc agccgccagc gtcgcccgtc tcgtcaaaca gatacgacag gaaggcgtgc
     3901 gtgccttgtt cgtagagaac atcaccgact cgcgactgat cgagcagatc gcgcgtgaag
     3961 gcggcgccgt ggtcggtggc cgcttgtatt ccgatgcctt gtcaccacca ggcaccgagg
     4021 ccgacaccta cctgaaattg ttcgcgcaca acgccagcgc catcgctgcc agcttgaagg
     4081 gtcgatcacc atgagcctga ttcccgtcac catcgtcacc ggcttgccgt gcagcggcaa
     4141 gagcacgctg atccagcgcc tgatccagca tcagcgcgac gagcgcatcg tctggatcgc
     4201 caagtcgttc gaagcgacca ccattccctt cgatatcgac ttgtgggtgc tcagcgtcga
     4261 agagcaggct gtagaaatag acaacggaga catgcgctgt gtcgttcgca gtgacctgct
     4321 gcacattctt caaacgctgc aaagtcagcg ctctgcaggg gtcttgcgat tcgaccgcgt
     4381 gatcatcgag accagcggct tcgccgaacc cgcgccggtg gtgcagacct tcttcgccga
     4441 ggaatcgatc tgtgaccagt accgactcga tgcggtcgtc acgctgctcg atgccgaaca
     4501 cgcgatggca cagctggatg cgcaccgcga agcccaggac caggtgggct ttgccgatcg
     4561 catcgtgctg acgagaacag accgcattgc agtcgatgca tcgcgcgccc tgcaccgacg
     4621 cctgacgcgc atgaacccgc gcgctcaggt gctgtcgaga gaaggtgacc tcgagatggc
     4681 acagcaactg ctcgatattc gcggcttcgc actgactggc gtgctcgaca tcgatgcgga
     4741 tggactggtc gatgcgaaga acgcgcgaga tggggatgtg acgagctttg tgttccgcac
     4801 ccggcaatcc ttcgacaagc agcgggtcga ggacttcttc agcagcatgc ttctggtcta
     4861 cggtgatcga c
//
LOCUS       DAOORV010000239         4835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_20981_length_4835_cov_6.167782, whole genome shotgun sequence.
ACCESSION   DAOORV010000239 DAOORV010000000
VERSION     DAOORV010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20981_length_4835_cov_6.167782"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKV56_18150"
     CDS             complement(<1..508)
                     /locus_tag="PKV56_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOX69774.1"
                     /translation="MYQHIKVPDNGEKITVNADFSLNVPDHPIIPYIEGDGTGFDITP
                     VMIKVVDAAVAKVYAGKRQIRWMEIYAGEKSTQIYGPDVWLPEETLTVLRDYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPIQYFTGVPSPVKEPEKTNMVIFRENSEDI
                     YAGIEYEAE"
     gene            565..1125
                     /locus_tag="PKV56_18155"
     CDS             565..1125
                     /locus_tag="PKV56_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOX69775.1"
                     /translation="MSQFNSSSAPCAVRAPGPCFDHGITPASARFGHAPGPGRRLRWS
                     LIVWLAAWHFTPAGAQGAAQSLPTLTLNAGIHNITAEVATTEEQRATGLMHRETMQPN
                     HGMLFAFEQAGQQCFWMKNTLLPLDIAFVADDGTIVNVDRMKPQTLDPHCSSRPVRFV
                     LEMNEGWFAKRGITAGSRLKGKPFGP"
     gene            complement(1203..1784)
                     /locus_tag="PKV56_18160"
     CDS             complement(1203..1784)
                     /locus_tag="PKV56_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOX69776.1"
                     /translation="MEHTLPALPYAIDALAPHLSKETLEYHHGKHHNAYVVNLNNLQK
                     GTEFESLGLEEIIKKSSGGVYNNAAQIWNHTFFWHGMKPSGGGEPKGALAAAINAKFG
                     SYAAFKEAFTKSAVGNFGSGWTWLVKKADGSVDIVNTGAAGTPLTTADKALLTIDVWE
                     HAYYIDYRNMRPKWVETFLTSLVNWDFAESNFA"
     gene            complement(1884..3197)
                     /gene="xseA"
                     /locus_tag="PKV56_18165"
     CDS             complement(1884..3197)
                     /gene="xseA"
                     /locus_tag="PKV56_18165"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOX69777.1"
                     /translation="MVDAPDAGFQRVVWGVASLVHAIGDTLAARFGACTVRGELSGYT
                     RASSGHSYFSLKDANGDSASIRCAMFKRAASLLQFQPADGQQVQLRGRVAVYEPRGEL
                     QFIVESMQRSGEGSLYEQFLRLKQKLELEGLFAAERKRPVVTHPRRVGVVTSLAGAAL
                     HDVASALARRSPHVELIVYPSVVQGSEAPAALVAAIEAAGRRREVDTLLVCRGGGSME
                     DLWSFNDERVVRAVASSPIPVISGVGHETDTTLTDFAADLRAPTPTAAAELCAPATAD
                     SLALLSASALGMRRRVHRVIETEAQRLDGLAMRSLRPAEAVRRQLQTLDLWSHRLASA
                     TRRAAVAGRHDLQAAAERLERASRVPTQRAQQTLARLEARLGGLDPAGVLRRGYAWLS
                     DGSGRALVSTSQLSVGQSVQAVLSDGVAEARVTAVNPVPGSTGAA"
     gene            3596..4231
                     /locus_tag="PKV56_18170"
     CDS             3596..4231
                     /locus_tag="PKV56_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX69778.1"
                     /translation="MFSIIQAAGWPIWPLIFCSVVALTLVIERLSSLRQTKVAPPRLL
                     EEVVSVTRASLPSADVVNKLAENSVLGTVLACGVRAVIAEPRITEPALREVFESAGRA
                     AVHRLERYLSPLGTIASAAPLLGLLGTVIGMIEIFGSQAPTGGSNPAALAHGISVALY
                     NTAFGLMVAIPSLIAYRHFRGLVDAYTLELEQACDRLTPHLMRFSVPRAAA"
     gene            4251..4679
                     /locus_tag="PKV56_18175"
     CDS             4251..4679
                     /locus_tag="PKV56_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX69779.1"
                     /translation="MNFRKARPEDPEINLIPFIDVLLVVLIFLMLSTTYSKFTELQVN
                     LPSADADKPRDNPREVIVGVSADGRYVVNGQPVDGRSVELLTAELGKAAGGPGDVPVI
                     VSADATAAYQSVVNVMDAARRAGLPRLTFAAQRNPAEGGR"
BASE COUNT          813 a         1600 c         1566 g          856 t
ORIGIN      
        1 actcggcctc gtactcgatg ccggcgtaga tgtcttccga gttctcgcgg aagatgacca
       61 tgttggtctt ctcgggctcc ttcacgggcg aaggaacgcc ggtgaagtac tggatcgggc
      121 gcaggcagac gtagaggtcg agttcctggc gcagggccac gttcagcgag cggatgccgc
      181 cgccaaccgg cgtcgtcagc gggcccttga tcgacacgac gtagtcgcgc agcaccgtca
      241 gcgtctcttc gggcagccag acatcggggc cgtagatctg ggtcgacttc tcgccggcgt
      301 agatttccat ccagcggatc tggcgcttgc cggcatagac cttggcgacc gccgcatcga
      361 ccaccttgat catcaccggt gtgatgtcga accccgtgcc gtcaccttcg atgtacggaa
      421 tgatcgggtg atctggaacg ttcagtgaaa agtcagcatt gacggtgatc ttttcgccgt
      481 tgtccggcac tttgatatgt tggtacatgg actgatggct ccgcagtcgt gttggggaca
      541 gaaaatctcg taggaaggat atcgatgtcg caatttaatt ctagttcagc accgtgtgcg
      601 gttcgagcgc cgggcccgtg cttcgaccac gggataacac ccgcgtcagc gcggttcggg
      661 cacgcaccgg gcccgggccg tcgcctgcgc tggagcctga tcgtctggct tgccgcctgg
      721 catttcacgc cggccggggc gcagggtgct gcgcagagcc tgcccacgct gacgctgaac
      781 gccggcatcc acaacatcac cgcagaggtg gcgacgaccg aagagcagcg cgccacaggg
      841 ctgatgcatc gcgagaccat gcagccgaat cacggaatgc tgttcgcctt cgagcaggcc
      901 ggtcagcagt gcttctggat gaagaacaca ttgctgcccc tggacatcgc cttcgtggcg
      961 gacgacggca cgatcgtgaa cgtcgaccgg atgaagccgc agacactgga tccgcactgc
     1021 tcgtccaggc cggtgcgctt cgtgctcgaa atgaacgagg gctggttcgc caagcgcggg
     1081 atcaccgccg gcagccgact caagggcaag ccgttcggac cttgaactga ccgccccgca
     1141 cccgcggcgg cccgcaaaaa agccggtcag cgaccggctt ttttgcttgc agggccgcag
     1201 gatcaggcga aattggattc cgcgaagtcc cagttcacca gcgaggtcag gaaggtctcg
     1261 acccacttcg gacgcatgtt gcgatagtcg atgtagtaag cgtgttccca cacgtcgatg
     1321 gtgagcagcg ccttgtcggc ggtggtcagc ggcgtgccgg cggcgccggt gttgacgatg
     1381 tccacgctgc cgtcggcctt cttgaccagc caggtccagc cggatccgaa gttgccgacc
     1441 gcgctcttcg tgaacgcttc cttgaacgcc gcgtagctgc cgaacttggc attgatcgcg
     1501 gccgccaggg cacccttggg ctcgccgcca ccgctcggct tcatgccatg ccagaagaac
     1561 gtgtggttcc agatctgcgc agcgttgttg tagacgccac cggaggactt cttgatgatt
     1621 tcctcaaggc ccaggctttc gaactcggtg cccttctgca ggttgttcag gttcaccaca
     1681 taggcgttgt ggtgcttgcc atggtggtac tccagcgttt ccttgctgag gtgaggagcc
     1741 agtgcatcga ttgcgtacgg cagtgccggc agggtgtgtt ccatcgtgat cctttcggtt
     1801 ggggtgtgtg acccattgga gaactgtttt cagctttcga ttgtaggcag cgaggccgaa
     1861 tccgacctct cgtagaggac tcgtcaggcc gcaccggtgg agcccggcac cgggttcact
     1921 gcagtgaccc gggcctcggc aacgccatcc gaaagcacgg cctgcaccga ctggccgacg
     1981 ctgagctgcg acgtcgacac cagcgcgcgg ccgctgccgt cgctgagcca ggcgtagccg
     2041 cgccgcagca cgcccgcagg atccagtccc ccaagccgcg cctcgagccg cgcgagggtc
     2101 tgctgcgcac gctgcgtcgg cacgcgactc gcgcgctcga ggcgctcggc cgccgcctgc
     2161 agatcgtggc ggcccgccac ggcagcgcgc cgggtggcgc tcgccagccg atgcgaccac
     2221 agatccagcg tctgcaactg acggcgaacc gcctcggcgg ggcgcagcga ccgcatcgcg
     2281 agtccatcga gccgctgtgc ctcggtctcg atgacgcggt gcacccggcg ccgcatgccc
     2341 agcgcgctcg cagacaacag cgccaggctg tccgcagtcg ccggtgcgca gagctcggcc
     2401 gcagccgtcg gcgtcggcgc acgcagatcg gccgcgaagt cggtgagcgt ggtgtccgtc
     2461 tcgtgcccga caccgctgat caccggaatc ggcgaactgg cgacggctcg cacgacgcgc
     2521 tcgtcgttga acgaccacag gtcttccatc gagccgcctc cgcgacacac cagcagcgtg
     2581 tctacctcgc gccggcgccc ggccgcctcg atcgcggcca ccagcgcggc aggcgcctcg
     2641 ctgccctgga ccacgctcgg atagacgatc agctcgacat gcggggaacg gcgagccagc
     2701 gccgaggcca cgtcgtgcag cgccgcacca gccagggagg tgacgactcc gacgcgtcgg
     2761 ggatgggtga ccaccggccg cttgcgctcg gcagcgaaca gtccttcgag ttcgagcttc
     2821 tgcttgagac gcaagaactg ctcatacagc gatccttcac cgctgcgctg catggattcg
     2881 acgatgaact ggagctcgcc acggggctcg tagacggcca ctcgaccccg cagctgcacc
     2941 tgctgaccgt ctgccggctg gaactgcagc aggctggctg cgcgcttgaa catggcgcag
     3001 cggatcgagg ccgaatcacc gttggcatcc ttcaggctga agtagctgtg gccgctcgaa
     3061 gcccgtgtgt agcccgagag ttcgccgcgc accgtgcagg cgccgaagcg agcagccagc
     3121 gtgtctccga tggcgtggac cagactggcg acgccccaca ccacgcgctg aaaaccggcg
     3181 tccggcgcat caaccacgcg caaacccaat atccatgcgg gtcgagaagt ccacaaccgc
     3241 gcccgtcaag gcacaggggc ccgcggcaac tgtcatggga cttacacaat ctctcaagat
     3301 gttgatttgc atggagttat tactgcgcaa aacggaggca aatcgttcgg gaggccgtga
     3361 aatcgcgggg ttttcgattt cggacacgag atacccacaa agttatccac acattcggtg
     3421 gacagggcac ggcatggaca tgaaccccga agcaaccgcg aatcctcggg ccaccccgtt
     3481 gctgacccgg gacactcgat gtcaccgaac agcgccatga caggcgccgc tgcaggccat
     3541 aatcgccccg ctagcgagcc ctgttcttcc acgccacaga gcggaaagta ccgacgtgtt
     3601 ttcgatcata caagccgctg gatggccgat ctggcccttg attttctgtt cggttgtcgc
     3661 gctgaccctg gtcatcgaac ggctttccag cctgcggcag acgaaggtcg cgccgccgcg
     3721 cctgctggaa gaggtggtgt cggtgacgcg cgcgagcctg ccgtcggccg acgtcgtcaa
     3781 caagctggct gagaactcgg tgctcggcac cgtgctggct tgcggtgtgc gagcggtgat
     3841 tgccgagccg cgcatcaccg agccggcctt gcgcgaggtc ttcgaatccg ccggccgcgc
     3901 cgccgtccac cgtctcgagc gctacctcag cccgctgggc acgatcgcat cggccgcgcc
     3961 cctgctgggt ctgctgggca ccgtcatcgg catgatcgag atcttcggct cgcaggcgcc
     4021 gaccggtggc agcaatccgg cggccctcgc ccacggcata tcggtcgcgc tctacaacac
     4081 cgctttcggc ctgatggtgg ccattccgtc gctgattgcc taccggcact tccgcggcct
     4141 ggtcgatgcc tacacgctgg agctggagca ggcctgtgat cgcctgaccc cgcacctgat
     4201 gcggttctcc gtgcctcgcg cggcagccta ggagccatcg ggcgatggcg atgaatttcc
     4261 gcaaggcgcg cccggaggat ccggagatca acctgatccc gttcatcgac gtgctgctgg
     4321 tggtcctgat cttcctgatg ctgtcgacca cctactcgaa gttcaccgaa ctgcaggtga
     4381 acctgccgtc tgccgatgcc gacaagccgc gcgacaaccc gcgtgaagtc atcgtggggg
     4441 tgtctgccga cggccgctac gttgtcaatg gccagccggt cgatggccgc agcgtcgagt
     4501 tgctcacggc cgagcttggc aaggcggccg gcgggccggg cgacgtgccg gtcatcgtgt
     4561 cggccgacgc gacggcggcg taccagtcgg tggtcaacgt gatggatgcg gcccgccgcg
     4621 ccgggcttcc ccggctgacc ttcgccgcgc agcgcaatcc ggccgaaggc ggccgctgac
     4681 ggtgcgttcg tccggcctcg aagccgcgct gcaaagggcg tggctgcgtc gaggcgcgct
     4741 ggccctggcg ctctggccgc tgtcgatgat cctgcgcctg ctggtgggcc tgaggaaatc
     4801 cctttacatg ctgggactgc gcaagccggt ttcgc
//
LOCUS       DAOORV010000240         4818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21063_length_4818_cov_7.816922, whole genome shotgun sequence.
ACCESSION   DAOORV010000240 DAOORV010000000
VERSION     DAOORV010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21063_length_4818_cov_7.816922"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /gene="chrA"
                     /locus_tag="PKV56_18180"
     CDS             complement(<1..1003)
                     /gene="chrA"
                     /locus_tag="PKV56_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOX69780.1"
                     /translation="MTASSPSLQPIVEPDVPSTGEPPAAVGFAQALQFWLKLGFISFG
                     GPAGQIALMHTELVERRRWISEQRFLHALNYCMVLPGPEAQQLATYIGWLMHRTWGGI
                     VAGALFVLPSLFILIALSWIYLRFGDVPLVAGIFYGIKPAVTALVVHAAHRIGSRALK
                     NGWMWGIAAAAFVAIFAFDTPFPAIVLAAGLIGHFGARLAPQVFALGGGHGSAKKSYG
                     RALIDDDTPTPAHARFSRRHLAKVLAIGLGLWAAAMATLLATQGLQGTLTQMGWFFTK
                     AALLTFGGAYAVLPYVYQGAVEHHQWLSGAQMIDGLALGETTPGPLIMVVAFVGFVGG
                     "
     regulatory      1138..1223
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1264..3105
                     /locus_tag="PKV56_18185"
     CDS             1264..3105
                     /locus_tag="PKV56_18185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOX69781.1"
                     /translation="MKLRNILSAPAILATTVFILAGHAHARFNPGVEATPEAPVALVP
                     ADTTDRLIIKYRKSQTANTALRSRHAVTVAGNRQGVLVSEFRRLHNGDQVLQLSRRMQ
                     PGELDNLIQSLRTGDANIEYAEPDRILQPLYVPNDRLYPQQWALSDATAGIRAPAAWD
                     RTTGTGVTIAVIDTGVRRHADLAANLLPGYDFVTSPSIGADGGGRDADATDPGDAAPA
                     GACGVGSAASNSSWHGTHVAGLIAAAGGNGVGVTGVAFGARILPLRAMGRCGGYTSDV
                     ADAIVWAAGGSVGGLPLNTHPAKVINLSLGGAGTCGITSQNAINAARAKGATVVVAAG
                     NANSGTNGISPANCAGVIAVAATGRSGGKAWYSSFGSHVTVAAPGGESGAGLLSTLNA
                     GSSLPGADSYAYYMGTSMAAPMVSGVAALMLSVNPTLTPDQIALLLKASARPFPALCS
                     GCGAGLVDAHAAVSQALAARGTTPTPPATPAPAPAAPQWPVASSNATAIKEAEPNNSI
                     AAAQVIGSASSIISGAIGNSADSDYYRFPIAPGSKVTLTMTAGAASGFGLGMYTTSGR
                     LLLLNPGVTGRQQRMTVSNSGSGAAWLVVRVLRTTGNTGSYKLGLSS"
     gene            complement(3175..>3618)
                     /locus_tag="PKV56_18190"
     CDS             complement(3175..>3618)
                     /locus_tag="PKV56_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOX69782.1"
                     /translation="HPESVQAGAHSVKGAASTIGAVHVSQLAHDLELAAADRKPAAEL
                     EAGCAELVGALQSLVLSISEAMPQGESAPMPQDDKPASTDELDQLESLLQQADYRSVG
                     EFRKLAPALRRQYGAPVADIGTCLRNFDYERALTLLRGLRVEESR"
     assembly_gap    3619..3718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3719..4804)
                     /locus_tag="PKV56_18195"
     CDS             complement(<3719..4804)
                     /locus_tag="PKV56_18195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69783.1"
                     /translation="MSHEIRTPMNAIIGLTHLMRRDSRDAGLTDRLGKVSDAANHLLQ
                     VINDILDLSKIDAGKVELEQVDFSLKRMMDRTCALVVDRAKAKGLAFSVDLDHLPDDL
                     RGDPTRLSQALINLLSNAVKFTERGSIAVTGELLEHDDESMLIRFAVRDTGVGISPEV
                     KSHLFQAFEQADTSTTRRFGGTGLGLAITRRLAVLMGGDVGVSTEVGVGSEFWFTARL
                     QAGEPTQVDALLARDQSESDWRSQGLSGRLLLVEDNPVNRELAIELLESIGLHTEVAV
                     DGVEAVERMRTGSYDLILMDVQMPRMDGLEATRRIRELPGCATLPILAMTAGAFGEDR
                     DACKAAGMNDHIAKPVDPERLYSALHQW"
BASE COUNT          763 a         1718 c         1572 g          665 t
ORIGIN      
        1 agccaccgac gaagccgacg aaggcgacca ccatgatcag cggccccggc gtggtttcgc
       61 caagcgccag gccgtcgatc atctgcgcgc cgctcagcca ctggtggtgc tcgaccgccc
      121 cctggtacac gtagggcagc accgcatacg ccccgccgaa ggtcagcagc gcggccttcg
      181 tgaagaacca gcccatctgc gtcaaggtgc cctgcaggcc ctgcgtggcg agcagcgtcg
      241 ccatcgccgc cgcccacagg cccagcccga tcgcgagcac cttcgccagg tggcggcgcg
      301 agaaacgcgc atgcgccggc gtcggcgtgt cgtcgtcgat caaggcccgg ccgtagctct
      361 tcttcgcgct gccatgaccg ccgccgagcg caaacacctg cggcgccagc cgcgcaccga
      421 agtggccgat cagaccggcc gcgagcacga tggccgggaa cggtgtgtcg aaagcgaaga
      481 tcgcgacgaa tgccgccgcg gcaatgcccc acatccagcc gttcttcagc gcgcggctgc
      541 cgatgcggtg ggccgcgtgc accaccagcg ccgtgaccgc cggcttgatg ccgtagaaga
      601 tgccggcgac cagcggcacg tcgccgaagc gcaggtagat ccacgacagc gcgatcagga
      661 tgaacagcga cggcagcacg aacagcgcgc cggcgacgat gccgccccag gtgcggtgca
      721 tcagccagcc gatgtaggtc gcgagctgct gcgcctcggg acccggcagc accatgcagt
      781 agttcagcgc atgcaggaag cgctgctcgc tgatccagcg gcggcgctcg accagttcgg
      841 tgtgcatcag cgcgatctgg ccggccgggc caccgaagct gatgaagccc agcttcagcc
      901 agaactgcag cgcctgtgcg aagccgaccg ctgcgggcgg ttcgcccgtc gaaggcacgt
      961 cgggctcgac gatcggctgc agcgaaggcg atgaagcggt catgcgcggc attgtgcggg
     1021 ctgggccgca ccgccgacac agcccgcaac gcaccgaccg cgccccgggt gccgcgaatg
     1081 gctcgtgcct gcgagcagac cttcagacgc cgcgcgcggg gccgataacc ggtctgaagc
     1141 tcatttccgc aaaccgcgcg aaagcgcggg gcgcaaagcc accggcctac agcgcacagc
     1201 gccacggcag cggggctgcc ggcccgccga cccgggccgg cctcgaaccg acgaggcagt
     1261 cccatgaagt tgcgcaacat cctttcggca ccggccatcc tggcgaccac ggtgttcatc
     1321 ctcgccggcc acgcgcacgc gcgattcaac ccaggcgtcg aggcgactcc cgaggccccc
     1381 gtggccctgg tgccggcgga cacgaccgat cgactgatca tcaagtaccg caagagccag
     1441 acggcgaaca cggcgctgcg ctcgcgtcac gccgtcacgg tggccggcaa ccgccagggc
     1501 gtgctggtct ccgagttccg aaggctgcac aacggagacc aggtgctgca gctcagccgc
     1561 cgaatgcagc caggtgaact ggacaacctg atccagagcc tgcgcaccgg tgacgccaac
     1621 atcgaatacg ccgaacccga ccgtatcctg caaccgctgt acgtgcccaa cgacaggctg
     1681 tatccgcagc aatgggcgct gtcggacgcg acggcaggca tccgcgcgcc ggccgcctgg
     1741 gaccggacca ccggcaccgg cgtgaccatc gcggtgatcg acaccggcgt gcgccggcac
     1801 gccgacctcg cggccaacct gctgccgggc tacgacttcg tcaccagccc gtcgatcggc
     1861 gctgatggcg gcggacgcga cgcagacgcc accgaccccg gcgacgccgc gccggccggc
     1921 gcctgcggcg tgggctctgc ggcatcgaac agttcgtggc acggcaccca cgtcgcggga
     1981 ctgatcgcgg cggccggagg caacggtgtc ggcgtgacgg gcgtcgcgtt cggcgcccgc
     2041 atccttccgc tgcgcgcgat gggccgctgt ggcggctaca cctcggacgt ggccgacgcc
     2101 atcgtctggg ccgctggcgg ctcggtcggc ggcttgccgc tgaacaccca tcctgccaag
     2161 gtcatcaacc tgtcgctcgg cggggccggc acctgcggta tcacctcgca gaacgccatc
     2221 aacgcggccc gcgcgaaagg cgccacggtc gtcgtcgccg ccggcaacgc caactccggc
     2281 accaacggca tttcgccggc caactgcgcc ggcgtcatcg cggtggcggc caccgggcgg
     2341 tcgggtggca aggcctggta ctcgagcttc ggcagccacg tcaccgtggc cgctccgggc
     2401 ggcgagtccg gtgcaggact gctctcaaca ctgaacgccg gcagcagcct gcccggtgcc
     2461 gacagctacg cctactacat gggcacctcg atggccgccc cgatggtcag cggggtcgcc
     2521 gcactgatgc tgtcggtcaa cccgacactg acgcccgacc agatcgccct gctgctgaag
     2581 gccagcgccc ggcccttccc ggccctgtgc tccggctgtg gcgcgggcct cgtcgatgcc
     2641 catgcggctg tctcgcaggc cctggccgcg cgcggaacca cgcccacgcc gcccgcgact
     2701 ccggccccgg cccccgcggc gccccagtgg ccggtggcgt cgtccaatgc cacggcgatc
     2761 aaggaagccg agccgaacaa cagcatcgcc gcggctcagg tcatcggctc ggcgtccagc
     2821 atcatctcgg gcgccatcgg caacagcgcc gacagcgact actaccggtt ccccatcgca
     2881 ccgggcagca aggtcacgct gacgatgacg gccggtgccg cctccggctt cggactgggc
     2941 atgtacacga ccagcggccg actgctgctg ctcaatcccg gcgtgaccgg gcgccagcag
     3001 cgcatgacgg tctccaacag cggatcaggt gccgcgtggc tcgtggtgcg tgtactgcga
     3061 accaccggaa acaccggaag ctacaagctc gggctgtcgt cctgagtccc ggctgaaccg
     3121 gccgccgcag ccgcccgaga ccgggcgcca cgtgcggccg tcgcgcttcc aggcctagcg
     3181 ggattcctcg acgcgcaagc cgcgcagcag cgtcagcgcg cgttcgtagt cgaaattgcg
     3241 caggcaggtg ccgatgtcgg ccaccggggc gccgtactga cggcgcagtg caggcgccag
     3301 cttgcggaac tcgccgaccg accggtagtc ggcctgttgc agcaggctct ccagttgatc
     3361 gagttcgtcg gtggacgccg gcttgtcgtc ctgcggcatc ggcgcactct cgccctgcgg
     3421 catggcttcg ctaatggaca gcaccagcga ctgcagcgca cctaccagct cggcgcagcc
     3481 cgcttcgagt tcagcggccg gcttgcgatc ggcggcggcc agttccaggt cgtgcgccag
     3541 ctgcgagaca tgaaccgcac cgatggtcga tgcagccccc ttgaccgaat gggcgcccgc
     3601 ctgcaccgat tcgggatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     3721 actggtgcag cgccgaatag agccgctcgg ggtcgaccgg cttggcgatg tggtcgttca
     3781 tgccggcggc cttgcaggcg tcacggtctt cgccgaacgc gcccgccgtc atcgcgagga
     3841 tcggcagcgt cgcgcagccc ggcagttcgc gtatgcggcg ggtggcctcg agcccgtcca
     3901 tccgcggcat ctggacatcc atcaggatca ggtcgtagct gccggtgcgc atgcgctcga
     3961 cggcctcgac accgtcgacc gcgacctcgg tgtgcaggcc gatcgactcc agcagttcga
     4021 tcgcgagttc gcggttcacc gggttgtcct cgaccagcag cagtcgcccg ctcaggccct
     4081 gactcctcca gtccgactcc gactgatcgc gtgcgagcag cgcatcgacc tgggtcggct
     4141 cgccggcctg cagccgggcg gtgaaccaga actcgctgcc gacgccgacc tcggtgctga
     4201 cacccacgtc gccgcccatc aggacggcca ggcgccgggt gatcgcgagc ccgaggccgg
     4261 tgcccccgaa gcgccttgtg gtcgacgtgt cggcctgctc gaatgcctgg aacaggtgac
     4321 tcttcacctc gggcgaaatg ccgaccccgg tgtcgcgcac ggcaaagcgg atcagcatcg
     4381 actcgtcatc gtgctcgagc aactcgccgg tgaccgcgat gctgccgcgc tcggtgaact
     4441 tcacggcgtt gctcaacagg ttgatcaagg cctgcgacag ccgggtcgga tcaccgcgca
     4501 agtcgtccgg caggtggtcc agatcgaccg agaacgccag gcccttggcc ttggcacgat
     4561 caaccaccag cgcgcaggtg cggtccatca ttctcttcag cgagaagtcg acctgctcga
     4621 gctcgacctt gccggcgtcg atcttcgaca ggtcgaggat gtcgttgatc acctgcagca
     4681 ggtgattggc ggcgtccgag accttgccca gccgatcggt cagccccgcg tcgcgggagt
     4741 ctcgccgcat caggtgggtc agcccgatga tggcgttcat cggcgtgcgg atctcgtggc
     4801 tcatgttggc caggaagg
//
LOCUS       DAOORV010000241         4808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21125_length_4808_cov_8.900694, whole genome shotgun sequence.
ACCESSION   DAOORV010000241 DAOORV010000000
VERSION     DAOORV010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21125_length_4808_cov_8.900694"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKV56_18200"
     CDS             complement(<1..724)
                     /locus_tag="PKV56_18200"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOX69784.1"
                     /translation="MATIPRRAYAEMFGPTVGDRLRLADTDLIIEVEQDYTLRAGSYG
                     EEVKFGGGKTIRDGMGQSQRVNGPGDHEAVDCVLTNALILDHWGIVKADIGLRGGRVA
                     AIGKAGNPDVQPGIDIVIGPGTEIIAAEGMIVTAGGIDSHIHFICPQQIEEALMSGVT
                     TMLGGGTGPATGTFATTCTPGPANIARMLQAADALPMSLGFFGKGNASQPAALRQQVD
                     AGVIGLKLHEDWGTTPAAIDCCL"
     gene            complement(759..1064)
                     /locus_tag="PKV56_18205"
     CDS             complement(759..1064)
                     /locus_tag="PKV56_18205"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HOX69785.1"
                     /translation="MIPGELLCDGPDHQINVGRRTITVVVENSGDRPIQVGSHYHFAE
                     TNAALVFDRAAARGMRLNIASGTAVRFEPGQQRTVALVDFAGDRQVFGFRGQVQGAL"
     gene            complement(1061..1228)
                     /locus_tag="PKV56_18210"
     CDS             complement(1061..1228)
                     /locus_tag="PKV56_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69786.1"
                     /translation="MKEAIAWLALTAASSAFAHEGHGLGSGSHWHASDAWGYIALVAV
                     LAVALWAGRKK"
     gene            complement(1250..1552)
                     /locus_tag="PKV56_18215"
     CDS             complement(1250..1552)
                     /locus_tag="PKV56_18215"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HOX69787.1"
                     /translation="MELTPREKDKLLIFTAALLAERRRARGLKLNYPEAVALISAAIM
                     EGARDGKTVAELMSDGKLVLTRADVMDGVAELIPEIQMEATFPDGTKLVTVHQPIT"
     gene            complement(1680..2576)
                     /locus_tag="PKV56_18220"
     CDS             complement(1680..2576)
                     /locus_tag="PKV56_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOX69788.1"
                     /translation="MSLHPESDRPVVLVVDDAPSSLGVLCDTLESAGYTVLVAGDGES
                     ALQRLELVTPDAILLDGLMPGLSGFDTCRRIKGTPAWAHVPVIFMTGLSETANVVEGF
                     ACGGVDYVVKPLRAQEVLARLHTHARNARITRMARDAVDVAGLGVVFVDALGRVAWRS
                     PQAAVWLLALEDDAGGGHLPLALQAALDVDSTQVVRTQTDPPLSVRNMGTATLGETML
                     LLELQREGAATSSSRLSHAALTPRETEVLSWLAKGKTNRDIADILGMSPRTVNKHLEH
                     IFEKLGVETRAAAAALASGHLP"
     gene            complement(2578..>4808)
                     /locus_tag="PKV56_18225"
     CDS             complement(2578..>4808)
                     /locus_tag="PKV56_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX69789.1"
                     /translation="LGLYSNVAIAWVGALVADLVINKPLGWSPAGIEFKRAHLYDINP
                     VGLLSMLLAAALAVTAYAGTLGPQAQAFAPFIALITAMVLSPLLAWWTQGRYYIARPP
                     QDLGRPGQLLKCVVCENRFETEDMAHCPAYGAPICSLCCTLESRCHDRCKTGSAAAEQ
                     VEGLLTSLLPKELSTRVNFRVGHYLVVALSLCTLLAVLMSMVYTQEVGPDLAPETQAL
                     LEGALVKVFAMLLLVVAVCSWWVVLGSESRRLAQDESNRQNQLLQREIEAHSRTDAAL
                     QAARDVAESANQAKTRYVAGMTHELRTPLNSILGYSQILLKDESLTSTPREAVQTIQR
                     SGEHLLALIDGLLDLARIEAGRLQLEPMPLHLPDFLDELVRMVSPQAEAKGLAFVYTR
                     DGHLPTWVKADAKRLRQILINLLANAVRFTDTGTVTLHVDSRKQVVRFDVVDTGVGVL
                     PQDRERIFLPFERGAAGRRQGGEPGTGLGLTITGLLTSLMGGELMLTSTSSQGSTFSV
                     RVYLNETSDPGPMREVPRQIVGYFGERRTLLVVDDQPVQRQMLAGMLMPLGFDVREAA
                     SGGECLASLHESVPDAILLDITMDDMDGWQAATRLRAAGYTDVPIIMVSANVFENQVQ
                     RLRAAQCQGFVAKPVLESELIAALHRTLGLEWRAAGAVSLPEDLGRLTAGQLNLPDRV
                     RTDLLRLARVGHVRGLQTALDGIAAADPSLAAACTRLRGLVSRFEMDRLQSLLMDESE
                     VT"
BASE COUNT          750 a         1597 c         1645 g          816 t
ORIGIN      
        1 tcaggcagca gtcgatggcg gcgggtgtgg tgccccagtc ctcgtgcagc ttcaggccga
       61 tcacaccggc gtcaacctgc tgtcgcaatg cggccggctg gctggcgttg cccttgccga
      121 agaagccgag actcatcgga agcgcatcgg cggcctgcag catgcgtgcg atgttggccg
      181 gacccggcgt gcaggtggtg gcgaacgttc ccgttgccgg gccggtgccg ccgccgagca
      241 tcgtggtcac gccgctcatc agtgcctcct cgatctgctg cgggcagatg aagtggatgt
      301 ggctgtcgat gccacccgcg gtgacgatca tcccttcggc cgcgatgatc tctgtacccg
      361 gtccgatgac gatgtcgatg cccggctgca catcgggatt gccggccttg ccgatcgctg
      421 cgacgcgtcc gccgcgcagg ccgatgtcgg ccttgacgat gccccagtgg tcgaggatca
      481 gtgcgttggt gagcacgcag tcgaccgcct cgtgatcgcc cgggccattg acgcgctgtg
      541 actgacccat cccgtcgcgt atggtcttgc cgccgccgaa cttcacttcc tcgccgtagc
      601 tgcccgctcg cagcgtgtag tcctgctcga cctcgatgat caggtcggtg tccgccagcc
      661 gaagccggtc accgacggtg gggccgaaca tctcggcata ggcgcggcgg gggatcgtgg
      721 ccatggggtt cctgcgaaat cgtgtcgggg tggggcgatc agagcgcgcc ttgaacctgc
      781 cctcggaagc cgaaaacctg acggtcgccg gcgaaatcga cgagtgcgac ggtgcgctgc
      841 tgaccgggct cgaagcgcac cgcggtgccg gacgcgatgt tcagccgcat gccgcgcgct
      901 gcggctcgat cgaacaccaa cgcggcgttg gtctcggcga agtggtagtg cgagccgacc
      961 tggatcggcc ggtctccgct gttctcgacc acgacggtga tggtgcggcg cccgacgttg
     1021 atctggtggt cggggccgtc gcagagcagt tcgccgggaa tcacttcttg cgccctgccc
     1081 acaaggcgac ggccaacacg gcgacgaggg cgatgtagcc ccaggcatcg gacgcatgcc
     1141 agtgcgatcc cgatcccagg ccgtgtccct cgtgcgcgaa agcgctggat gcggctgtca
     1201 gcgccagcca ggcgattgct tctttcatcg ggcgtctccg tcgagtgaat caggtgatcg
     1261 gttggtgaac ggtcaccagc ttggtgccgt ccgggaaggt tgcttccatc tggatctcgg
     1321 ggatcagttc ggccacgcca tccatcacgt cggcgcgcgt caacacgagc ttgccgtcgc
     1381 tcatcagttc cgcgaccgtc ttgccgtcgc gcgcaccctc catgatcgcg gcgctgatca
     1441 gcgcgactgc ttcggggtag ttgagcttca gaccacgggc acgccggcgc tcggcgagca
     1501 gcgcggcggt gaagatcagc agcttgtctt tttcgcgcgg ggtcagttcc atcgggtgtt
     1561 cgagtggttc gcgatgcgtg ggatggtagc ggccggtgcg gctgcagtga atacaagacc
     1621 gatgccagct cggggccggt ctcgagatgc cccgagcgcc gggcaccctg gacggtgact
     1681 catggcaagt gcccgctggc cagcgcggcg gcggcggccc gggtctcgac ccccagcttc
     1741 tcgaagatgt gctcgaggtg cttgttgacg gtgcgcgggc tcatgccgag gatgtcggcg
     1801 atgtcgcggt tggtcttgcc cttggccagc caggacagca cctcggtctc gcggggggtc
     1861 agtgcggcgt ggctcagacg cgatgaactg gtggctgcgc cctcgcgttg cagttcgagc
     1921 agcagcatcg tctcgccgag cgtcgcggtg cccatgttgc gcaccgaaag tggaggatcg
     1981 gtctgggttc gcacgacctg ggtgctgtcg acgtcgagcg cggcctgcag cgccagcggc
     2041 aggtggccgc cgcccgcgtc gtcctcgagg gccagcagcc acaccgccgc ctgcggagat
     2101 cgccatgcga cacggcccag cgcgtccacg aacaccacgc ccaggccggc cacatcgact
     2161 gcgtcgcgcg ccatgcgggt gatgcgcgca ttgcgtgcat gggtgtgcag gcgcgcgaga
     2221 acctcctgtg ctcgcagcgg cttgacgacg tagtcgactc caccacacgc aaagccctcg
     2281 acgacgttag ccgtctccga cagcccggtc atgaagatca ccggcacatg cgcccaggcc
     2341 ggcgttccct tgatgcggcg gcaggtgtcg aagccggaca gaccgggcat caggccgtcc
     2401 agcaggatcg cgtcgggcgt gaccagttcc agacgttgca gcgcgctctc gccgtcgccc
     2461 gccaccagca cggtgtagcc ggcactttcc agcgtgtcgc agagcacgcc caggctgctc
     2521 ggcgcgtcgt ccacgacgag caccacgggt cgatcggatt cggggtgcaa tgacatgtca
     2581 ggtgacttcg gattcatcca tcaacagcga ctggagccgg tccatctcga accggctgac
     2641 caggccacgc agtcgagtgc aggctgcggc gagcgagggg tctgcggccg caatcccgtc
     2701 gagggcggtc tgcaggccgc gcacatgacc gacgcgtgcc agtcgcagca ggtcggtgcg
     2761 cacgcggtcg ggcaggttca attgtccggc cgtgagcctg ccaaggtcct cgggcaggct
     2821 cacggcgcca gccgcacgcc attccaggcc gagcgtgcga tgcagtgcgg cgatcagctc
     2881 cgattcaagc accggcttgg ccacgaatcc ctggcactgg gccgcgcgca ggcgctggac
     2941 ctggttctcg aacacattgg ccgagaccat gatgatcggc acgtcggtgt acccggcggc
     3001 gcgcaagcgg gtcgcggcct gccagccgtc catgtcgtcc atcgtgatgt cgagcaggat
     3061 cgcgtccggc accgattcat gcaggctggc caggcattcg ccaccgctgg ccgcttcgcg
     3121 cacgtcgaag cccagcggca tcagcatgcc cgccagcatc tgccgctgca ccggctggtc
     3181 gtcgacgacc agcagcgtgc ggcgctcgcc gaagtagccg acgatctggc gcggcacctc
     3241 gcgcatcgga cccgggtccg aggtctcgtt caggtacacc cgaacgctga aggtgctgcc
     3301 ctggctcgat gtgctggtga gcataagctc tcctcccatc agcgaggtca gcaggccggt
     3361 gatcgtcagt cccagcccgg tgccgggctc gcctccttgt cgccggccgg ctgcgccgcg
     3421 ctcgaacggc aggaagatgc gctcgcggtc ctgaggcagc acgccgacac cggtgtcgac
     3481 gacatcgaag cgcaccactt gcttgcgcga gtcgacgtgc aaggtcacgg tgcctgtatc
     3541 ggtgaagcga accgcgttgg ccagcaggtt gatcaggatc tggcgcagac gcttggcgtc
     3601 ggccttgacc caggtcggca ggtggccgtc gcgggtgtag acgaaggcga ggcccttggc
     3661 ttcggcctgc gggctgacca tgcgcaccag ctcgtcgagg aaatccggca ggtgcagcgg
     3721 catcggttcg agctgcagcc ggcccgcctc gatgcgagcc aggtcgagca gtccgtcgat
     3781 cagcgccagc aggtgctcgc cgctgcgctg gatcgtctgc acggcttctc tcggtgtcga
     3841 ggtcagcgac tcgtccttga gcaggatctg cgaatagccc aggatgctgt tgagcggcgt
     3901 gcgcagctcg tgcgtcattc cggcgacgta ccgggtcttc gcctggttgg ccgattcggc
     3961 cacatcgcgc gccgcctgca acgcggcatc ggtgcgcgag tgcgcttcga tctcgcgctg
     4021 cagcagctgg ttctggcgat tcgactcgtc ctgcgccagt cggcggcttt cgctgccgag
     4081 aaccacccac cagctgcaca ccgccaccac gagcagcagc atcgcgaaca ccttgaccag
     4141 ggccccctcg agcagtgcct gcgtttccgg cgccagatcg gggcccacct cctgcgtgta
     4201 gaccatgctc atcagtacgg cgagcagggt gcacagcgac aacgcgacca cgaggtagtg
     4261 gccaacccgg aaattcaccc gggtcgagag ctccttggga agcagcgagg tcagcagccc
     4321 ctcgacttgc tctgccgcgg cggagccggt cttgcagcga tcatggcaac gcgattcgag
     4381 cgtgcagcac aacgagcaga tcggcgcgcc gtaggccgga cagtgcgcca tgtcttcggt
     4441 ctcgaagcgg ttttcgcaga cgacgcactt cagcagctgt cctggccggc ccaggtcctg
     4501 cggcggccgg gcaatgtagt agcggccttg ggtccaccat gccagcagcg gggacagcac
     4561 catcgcggtg atcagtgcga tgaacggtgc gaaagcctgc gcttgcggcc ccagcgttcc
     4621 ggcgtaggcg gtgaccgcga gggccgctgc cagcagcatc gacagcaacc cgaccggatt
     4681 gatgtcgtaa agatgcgccc gtttgaactc gatgccggcc gggctccagc ccaggggctt
     4741 gttgatcacc agatcggcca ccagcgcgcc gacccaggcg atggcgacgt tgctgtacag
     4801 gccgagca
//
LOCUS       DAOORV010000242         4792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21204_length_4792_cov_8.324045, whole genome shotgun sequence.
ACCESSION   DAOORV010000242 DAOORV010000000
VERSION     DAOORV010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21204_length_4792_cov_8.324045"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..403)
                     /locus_tag="PKV56_18230"
     CDS             complement(5..403)
                     /locus_tag="PKV56_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HOX69790.1"
                     /translation="MKNVSTWCILVAALMPLVCAGIAKWGTFSKPPSEGGFDNRHPRE
                     WIARQQGWRQRANAAQANCFEALPFFIGALLWAQQIGAPQQRVDLLAVSFILLRSAYV
                     ALYVLDRAGLRSLVWIAALAVNVALLFSGV"
     gene            complement(429..1016)
                     /gene="recR"
                     /locus_tag="PKV56_18235"
     CDS             complement(429..1016)
                     /gene="recR"
                     /locus_tag="PKV56_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOX69791.1"
                     /translation="MSEPALAALIDALRRLPGVGDKSARRMALHLLQHDRAGAQRLAM
                     ALQAAVANVRHCARCHTFTESEICGICADTGRNARLLCVVETPADQAAMERTGSYDGF
                     YFVLMGRLSPLDGIGVRDIGLQSLLSRVAEGGVEEVIVATNFTAEGEATAHAVSEMLK
                     SRGLLVTRLSRGVPVGSELEYVDLGTIAHALIDRR"
     gene            complement(1056..1382)
                     /locus_tag="PKV56_18240"
     CDS             complement(1056..1382)
                     /locus_tag="PKV56_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOX69792.1"
                     /translation="MMKGQLAGLMKQAQAMQDNLKKAQEELATIEVEGQSGAGLVKVT
                     MTCKHDVKRIVIDPSLLADDKDMLEDLVAAAFNDGLRRAEEVSQEKMGKLTAGMPLPP
                     GMKFPF"
     gene            complement(1426..3318)
                     /gene="dnaX"
                     /locus_tag="PKV56_18245"
     CDS             complement(1426..3318)
                     /gene="dnaX"
                     /locus_tag="PKV56_18245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOX69793.1"
                     /translation="MSYLVLARKYRPRSFDALVGQEHVVQALSNALTQGRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCTGADGRGGITASPCGVCSACRDIDAGRFVDYMELDAAS
                     NRSVEEITPLLDQAVYKPVVGRFKVYMIDEVHMLSNTAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPATVLSRCLQFNLRPMGMSTLRDHLAKVLAAEGIAAEPGALRLLARAARGS
                     MRDALSVTDQAIAFGGGALLEAGVRDMLGAVDRSHAIRLIEALAEHDGAALLAQADAL
                     RGLGLSATGTLAEVADLLQQMAVAQAVPDALDTSEADAVAATRLAPRLAADETQVLYS
                     MALHGRAELELAPDEHSGLVMVLLRMLAFCPPSGSRAVRPTHEGDQPAAAVAAPAASA
                     RRELPAASSSTSSGRSDLRHESRSVAVATAHSSSTPSAAAATSVAAEPTPPPWVDAEW
                     GDDSVAAPPVEPASGAAAAPICDVPTEAPALPADAMSGTSPSDVPVNAHLGDRWSEVV
                     QALSQSGSIVALARELALQSQCMRIDETESPATWVLQVERETLRSAAQREKLEAALAL
                     HLGTTVRIEVLAGPAVDTPASRMAAERERRQREAEQIIQDDPIVRALMTQYAGARIVP
                     GSVRYQ"
     gene            3419..4558
                     /locus_tag="PKV56_18250"
     CDS             3419..4558
                     /locus_tag="PKV56_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOX69794.1"
                     /translation="MSTPTSSAPDQGTLSYEQSGVNYDLIDPLKVTAQRAAAATGAHL
                     AAHGFSEVTASRGESAYVVDVGPFYLASIVECLGSKALVADEMQRLTGKSYYDAIAQD
                     TIAMAINDLITVGATPLVVQAYWAAGGSDWFADAQRAQALVTGWKAACDRCGVAWGGG
                     ETPALAGIVEAGRIDLAASCTGLIKPKERLSVGDRLAPGDAIVLLASSGIHANGLSLA
                     RKLVERLPQGYLTPVEPGVSALTYGDALLAPTVLYSPVTEALYRAGITPRYCANITGH
                     GWRKLLRHPALHTYRIHCVPDVPPVLTFIQQHARQDDLEAYSTLNMGAGFALFVAADQ
                     AERTVAVAEAEGVRAWVAGQVEAGPKRLLIEPLGLEFGDDALQLR"
     gene            4604..4700
                     /gene="ffs"
                     /locus_tag="PKV56_18255"
     ncRNA           4604..4700
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKV56_18255"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          812 a         1641 c         1564 g          775 t
ORIGIN      
        1 ctgatcagac gcccgagaac aacagcgcca cgttgacggc cagcgccgca atccatacca
       61 ggctgcgcag cccggcgcgg tcgagcacgt agagggccac gtaggcgctt cgaagcagga
      121 tgaagctcac cgccagcagg tccacgcgtt gctggggcgc gccgatctgc tgtgcccaca
      181 agagcgcgcc gatgaagaaa ggcagcgcct cgaaacagtt ggcctgcgcc gcattggcgc
      241 gctggcgcca gccctgctga cgtgcgatcc attcgcgcgg gtgacggttg tcgaagccgc
      301 cctcggacgg cggcttcgag aacgtccccc acttggcgat gccggcacac acgagcggca
      361 tcagggccgc gacgaggatg caccaggtgc tgacgttctt catggcgggt tgcttcccac
      421 aaacggtttc aacggcgatc gatcagcgcg tgcgcgatcg tgcccaggtc gacgtactcg
      481 agttcactgc ccaccggtac gccgcgcgac agcctggtca ccagcaggcc ccgagacttc
      541 agcatctcgc tgaccgcatg cgcggtggct tccccttcgg cggtgaagtt ggtggcgacg
      601 atcacctcct cgactccccc ttcggcgacg cggctcagca gcgactgcaa gccgatatcg
      661 cgcacgccga tgccgtcgag cggactcaac ctgcccatca gcacgaaata gaaaccgtcg
      721 tagctgccgg tgcgctccat ggcagcctga tcggccggcg tctcgaccac gcacagcaac
      781 cgggcgttgc gtccggtgtc ggcacagata ccgcagatct cgctttcggt gaaggtgtga
      841 caacgcgcgc aatgcctgac attcgcgacc gcagcctgca gcgccatcgc caggcgctga
      901 gcaccggctc gatcgtgctg cagcaggtgc aatgccatgc gtcgcgccga cttgtcgccg
      961 acacccggca accgacgcaa cgcgtcgatc agcgcagcga gcgcaggctc agacacgcgc
     1021 agtcaggagc tgtgacgaca cgcccgaggc gtggctcaaa acgggaactt catgccgggc
     1081 ggcaacggca tgccagcggt gagcttgccc atcttttcct gagagacctc ctcggcgcgt
     1141 cgcagaccat cgttgaaggc cgcagcgacc aggtcctcga gcatgtcctt gtcatcggcg
     1201 agcaggctgg ggtcgatcac gatgcgcttc acgtcgtgct tgcaggtcat cgtgaccttg
     1261 accaggccgg cgcccgactg tccctcgacc tcgatcgtcg caagttcttc ctgcgccttc
     1321 ttcaggttgt cctgcattgc ctgggcctgc ttcatcaagc cggccagttg acccttcatc
     1381 atggtgttgt ctccttggtg gaattcggta ggggttcagg ttggttcatt ggtagcgaac
     1441 cgagcccggc acgatgcgcg cgccggcgta ctgcgtcatc aacgcgcgca cgatcgggtc
     1501 gtcctggatg atctgctcgg cttcgcgctg gcggcgctct cgttcggccg ccatgcggga
     1561 tgcgggcgtg tcgaccgcgg gtccggccag tacttctatg cgaaccgtcg tgccgaggtg
     1621 cagagccagc gcagcttcga gcttttcgcg ctgagcagcc gaacgcaagg tctcgcgctc
     1681 gacctgcagc acccaggtgg caggagactc ggtttcgtca atgcgcatgc actgcgactg
     1741 cagcgccagt tcgcgggcca acgcgacgat gctgcccgac tgagacaggg cctgcaccac
     1801 ttcgctccag cggtcgccaa gatgcgcgtt gaccggcaca tcagaaggtg aagtgccgga
     1861 catggcatct gccggcaagg cgggcgcctc ggtgggcaca tcgcagatcg gcgcagcggc
     1921 ggctcccgaa gctggctcga cggggggtgc tgccacgctg tcatcccccc actctgcatc
     1981 gacccagggc ggcggggtcg gctcggcagc aacggaggtt gcggcagcag ccgacggggt
     2041 cgaactggag tgagccgttg ccacggcgac agaacgcgat tcgtggcgca gatcgctgcg
     2101 gccagaagac gtgctcgagc tcgcggcagg caactcgcgt cgtgctgaag ccgctggcgc
     2161 tgcaactgcg gccgccggct ggtcaccttc gtgcgtcggt cgaacagcgc gagatccgga
     2221 aggtgggcag aacgccagca tgcgcagcag caccatcacc agaccgctgt gctcgtcggg
     2281 cgccagttcc agttcggcac ggccgtgcag cgccatgctg tagagcacct gcgtctcgtc
     2341 ggccgccagg cgcggcgcca gccgggttgc agcgacagcg tcggcctcgc tggtatcgag
     2401 cgcatccgga acggcctgtg ccaccgccat ctgctgcagc agatctgcca cctccgcgag
     2461 ggtgccggtc gctgacaagc ccaggccgcg taacgcgtcg gcctgcgcca gcagtgccgc
     2521 accatcgtgt tccgccagcg cttcgatcag gcggatcgcg tggctgcggt cgaccgcacc
     2581 cagcatgtcg cgcacaccag cttcaagcag cgcgccgccg ccgaacgcga tggcctgatc
     2641 ggtgacagac agcgcatcgc gcatcgagcc gcgcgccgcg cgggccagca ggcgcagcgc
     2701 accgggctcg gcggcgatac cttcggcagc cagcactttg gcgagatggt ctcgcagggt
     2761 gctcatgccc atcggacgca gattgaactg caggcagcgt gacaacaccg tggccggcac
     2821 cttctgcggg tcggtggttg ccagcacgaa cttcaggtac tcgggcggct cctccagcgt
     2881 cttcagcatc gcgttgaacg cggtgttgga cagcatgtgc acttcgtcga tcatgtagac
     2941 cttgaagcga cccaccaccg gtttgtagac ggcctgatca agcagcggcg tgatctcctc
     3001 gacgctgcgg ttggaagcgg catcgagttc catgtagtcg acgaagcggc ctgcatcgat
     3061 gtcacggcag gcactgcaga ccccacacgg gctcgcggtg ataccgccgc gcccgtccgc
     3121 gccggtgcag ttgagcgatt tggccaggat gcgcgacacc gtcgtcttgc cgacaccgcg
     3181 ggtgccggtg aacaggtagg cgtggtgcaa acggccctgc gtcaaagcgt tcgagagcgc
     3241 ctgcaccacg tgttcctgac ccaccagggc atcgaaactt cgtggtcggt atttgcgggc
     3301 cagcaccagg taactcatcg aacgattcta gttgccggcc acctttcggc cgcgtcgagc
     3361 gagggctgcc ggataatcgc agcgcactca cagcctcgcc agccgcggcc catcccgaat
     3421 gtccacgccc acctcctcag cgcccgacca gggcacgctc agctacgaac aatcgggggt
     3481 caactacgac ctgatcgatc ccttgaaggt caccgcacag cgcgccgccg ccgccaccgg
     3541 cgcccacctg gcggcacacg gattcagcga ggtgactgcg tcgcggggcg agtcggcgta
     3601 cgtggtcgac gtcggcccgt tctacctggc cagcatcgtc gaatgtctcg gctcgaaggc
     3661 cctggtggcc gacgagatgc agcggctcac cggcaagagc tactacgacg cgatcgcgca
     3721 ggacaccatc gcgatggcaa tcaacgacct gatcaccgtc ggtgccacac cgctggtcgt
     3781 gcaggcttac tgggcagccg gcggttccga ctggttcgcc gatgcgcagc gggcgcaggc
     3841 actggtgacg ggctggaagg ccgcctgcga ccgctgcggc gtggcctggg gaggcggcga
     3901 aacgccagca ctggcaggca tcgtggaagc tggccgcatc gacctcgccg cctcgtgcac
     3961 cgggctgatc aagccgaagg aacgactgtc ggtcggcgat cgcctggcac cgggcgacgc
     4021 gatcgtgctg ctggcctcga gcgggatcca tgccaatggc ctgagcctcg cacgcaagct
     4081 ggtcgagcga ctgccgcagg gctatctgac accggtcgag cccggggtct ccgcgctgac
     4141 ctacggcgat gcgctgctgg cgccgaccgt tctgtactcc ccggtcacgg aggccttgta
     4201 ccgcgccggc atcacgccgc gctactgcgc caacatcaca ggccacggtt ggcgcaagct
     4261 gttgcggcac cctgcgctgc acacctaccg catccactgc gttccagacg tgccgccggt
     4321 tctgacattc atccagcagc acgcgcgaca ggacgacctg gaggcctaca gcaccttgaa
     4381 catgggcgca ggattcgccc ttttcgttgc agccgatcag gccgagcgca ccgtcgcggt
     4441 ggccgaagcc gagggcgtgc gcgcctgggt cgccggacag gtcgaagcgg gaccgaagcg
     4501 actgctgatc gagccgctcg gtctcgagtt cggcgacgac gcgttgcagt tgcgctgacc
     4561 ctcctgcccg ccggaccagc acccgtcacc tacaatcgtc gtcgacgggc ctccccgcat
     4621 ggaagcgcgg ccaaccgggt caggtcggga acgaagcagc cccaaccgtt gtgaccagtg
     4681 ccggggtcag gctcgtcacc cccgcgggag ttcatgcccc gctcagctcg cctgcttgaa
     4741 cgccatcacc gcctggttgc gcagggtgcg cagcagcgac agttccttct cg
//
LOCUS       DAOORV010000243         4765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21371_length_4765_cov_6.806157, whole genome shotgun sequence.
ACCESSION   DAOORV010000243 DAOORV010000000
VERSION     DAOORV010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21371_length_4765_cov_6.806157"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1288)
                     /locus_tag="PKV56_18260"
     CDS             complement(155..1288)
                     /locus_tag="PKV56_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69795.1"
                     /translation="MTSSHRLPSASAHTDPGFAIGWDFAHFRLVPPVEQLLPGNPVRD
                     GWEVGQAVFGLRTLRGTHHVKKWLQLRLGAWMRGKAFELVQVTPNFLERIDVDVCPVT
                     GEALTRGTGADTDASVDRVNNQAGYGAGNLAVMSVRAYRAKSAYDWRDAATFVQQIEA
                     GQLDEIDGLNAREWARLTALMSYCTPLSHAEAASLPLLVLPPPRLRVLNPVQAVQACL
                     TLCFARPDRAPKLDALVRTFPAGVQFEVRSFLTTLLARRIAAVPAVTSGQLRAALEGA
                     WCETLIIRRWQRVALRMTPASCETVAAALPTAASGLAPLRYLSFVQAADGWTLDTGGL
                     VLSEGRPESQGIKCAAVSLPAQPEARHQVKPLTASAADRSLAI"
     gene            1551..2531
                     /locus_tag="PKV56_18265"
     CDS             1551..2531
                     /locus_tag="PKV56_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOX69796.1"
                     /translation="MTKTARVYKIEMLIRNRGHVSFQTLLDDLEVSPATLKRDLDYLK
                     DQLGAPIVYDRFLNGYKFGEEYRGQKHELPGLWFSERELYSLLMAHQLLNDLDSDGVI
                     SRHLQPLLERIHLMLGTSEAETKALLKRVKIIGSAKRPVPSQFFELVGEALLKRRRLH
                     LRYLTRGRGEVSERDVSPQRLVHYRNTWYLDAWCHTREKLLRFALDAIERADTLEAKA
                     KDVALKTVEAELDGGYGIFAGGDPQWATLVFSSSAAQWVSREEWHPAQKCAWLDDATY
                     QLEVPFVNETELLMDVLRHGDQVSVVSPPSLVALIAHRLESASQHYKGRA"
     gene            2684..3181
                     /locus_tag="PKV56_18270"
     CDS             2684..3181
                     /locus_tag="PKV56_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxE family protein"
                     /protein_id="HOX69797.1"
                     /translation="MDSYLPTILQLRLVVGFLGERAQHGWWPTSFFGDYSMRSLEFVA
                     PKTATLAQYHGAVEAARRLHDEHLSVGSYHLFRLPEEVEQDLHALVQSSQGAEVISQL
                     PKDKESALSSLAKMANAGVTAAEGPVSVGSISAMSEPDTAKAIAGVYVKALSANVKAY
                     PYLVK"
     gene            3178..3969
                     /locus_tag="PKV56_18275"
     CDS             3178..3969
                     /locus_tag="PKV56_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HOX69798.1"
                     /translation="MTLALPPYTTQLQAGLGLVDETKTLLDIWSPGMTPPQLQAASLA
                     SGRFTAVTARRLRNVVIECFKPRYLVAGGAPAAHLKRLSTVLTAAELAQAMMLFTARA
                     NPILADFIRQVYWSRYAGSYTHVTNDDARAFVERAIDDGRTATRWSQTTIPRVSRYLT
                     GCCADYGLLERGQRSSRRILPFRIAFPVAAYLAYDLHFAGVGDNALLGHEDWMLFGLD
                     RQAVLSELKQLSLKGLLIVQAAGDVVRISWKHQIMESLLDVLTQS"
     gene            3950..4525
                     /locus_tag="PKV56_18280"
     CDS             3950..4525
                     /locus_tag="PKV56_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HOX69799.1"
                     /translation="MSSLKADFDELRERIRHGRDLGPASFEPVFYLVFPPEQILEVKR
                     QMPAWISKLHQEGWDVHTFSMAEHIWSVLKADPLWPLCAMEDRSAPLDWARTNKALAD
                     ILVGEGALLKRLESALEPLEGKHNAVMLVTDLEALHPFLRIGAIESQLMGKFHVPTVF
                     FYPGIRTGKTNLKFLGFYPADGNYRSVHVGG"
     gene            4553..>4765
                     /locus_tag="PKV56_18285"
     CDS             4553..>4765
                     /locus_tag="PKV56_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69800.1"
                     /translation="MNIRTLFDPSKDIFRNIEKVITYGASQESRLKSEISEYVVTESI
                     EEQFRKLLDRMQLAMESGGENEVGVWV"
BASE COUNT          909 a         1510 c         1471 g          875 t
ORIGIN      
        1 ctggatgcga accagaactt cgacggcgtt gtccgtgccg gagcacaggc cggcgcggcg
       61 aggtgtcagg acgacggtgg atgcaagcga aaggtgggca ttcatgggcg ctctccaggt
      121 caggatcgga gagtcatgtt tacaggcgac tcgctcaaat agcgagcgaa cggtccgccg
      181 ccgatgcggt caacggtttg acctgatgcc tcgcttcagg ctgtgcgggg agactgactg
      241 cagcgcattt gatgccctgg ctctcaggcc gaccttccga cagcaccagc ccgcccgtgt
      301 cgagcgtcca tccgtcggcg gcttgcacga agctcaggta gcgcagcggc gcaagtccac
      361 tcgccgcggt gggcagggcg gccgccacgg tttcgcagga cgccggggtc atccgcagcg
      421 cgacgcgctg ccatcgccgg atgatcaacg tctcgcacca agctccttcg agcgcggccc
      481 tcagctggcc cgaagtcacc gccgggacag ccgcgatccg acgtgccagt agagttgtca
      541 gaaatgaccg gacctcaaat tgaacacctg ccgggaaggt gcgcaccaga gcgtcaagct
      601 ttggcgctct atccggccga gcgaaacaga gcgtcaagca tgcctgaacg gcctggacag
      661 ggttcaagac ccgcaggcgc ggcggcggaa gcaccagcaa gggaaggctt gcggcctccg
      721 cgtggctgag cggtgtgcag tagctcatga gcgccgtcag tcgcgcccac tcgcgggcat
      781 tcaagccgtc gatctcgtcc agttggcccg cttcgatttg ttggacgaag gtggctgcat
      841 cgcgccagtc gtaagccgac ttggcccgat aggcccgcac gctcatgacg gcaaggttgc
      901 ccgcgccata gccagcctgg ttgttcaccc ggtcgacgga agcgtcggtg tccgcgccgg
      961 tgccgcgggt gagtgcctca cccgtgacgg ggcagacatc cacgtcgatg cgctccagaa
     1021 agttcggcgt cacctgtacc agttcaaagg ccttgccgcg catccatgcg ccgaggcgca
     1081 gttgaagcca cttcttgacg tggtgagttc cccgtagcgt ccgcagtccg aagaccgcct
     1141 gaccgacctc ccagccgtcg cgcaccgggt tcccgggcag caattgctcg accggaggta
     1201 ccagtcggaa atgggcgaag tcccagccga tcgcgaaacc ggggtccgtg tgggccgacg
     1261 ccgacggtag acggtggctt gacgtcatgg tggtgcacct gtgaacggcg ctcttggccc
     1321 ggcaggacag cttctttatc ctcaagctca agaagtgagc tggcgataaa actccactcg
     1381 ggttgagggt taccgcggat agctcatgta ttgagctcat cgtcaccaat gctggcgaga
     1441 tgacgccata ccaaactgtg cgaatgttgt gcccgagtct ggacgaagtc agcacaccag
     1501 cggctacagt tggtgacgct cacaggatga gcgaccaacc ccgccgtgtc atgaccaaga
     1561 ctgcccgcgt ctacaaaatc gagatgctca tccgcaaccg cgggcatgtg agctttcaga
     1621 cgctgttgga cgatctggag gtgtccccgg ccacgctcaa acgggatctg gactatctga
     1681 aggaccagct cggcgctccc atcgtctacg accgcttcct caatggctac aagttcggag
     1741 aggagtaccg gggccagaaa cacgagctgc ccgggctctg gttcagtgag cgggagctct
     1801 actcgctgtt gatggcccac caacttttga atgacctcga ctcagatggc gtgatcagcc
     1861 gccatctgca gcccctgctg gagcgcatcc acctgatgtt ggggacgagt gaggccgaga
     1921 ccaaagcgct gctgaagcgt gtgaagatca tcggctccgc gaagcgcccg gtgcctagcc
     1981 aattcttcga actggtcgga gaggctcttc tgaagcgcag acgcctccac ctgcgctacc
     2041 tgacgcgagg gcgcggtgag gtcagcgagc gcgatgtgtc gccgcaaagg ctggtccact
     2101 accgaaacac gtggtacctg gacgcttggt gccacaccag ggagaagctg ttgcgcttcg
     2161 cgctcgacgc catagagcgg gctgacacat tggaagccaa ggccaaggat gtggcgctga
     2221 agacggtcga ggccgaactg gacggtggct atggcatttt cgccggtggc gatccgcaat
     2281 gggcaacgct ggtcttctcg tccagcgcgg cgcagtgggt aagccgcgaa gagtggcatc
     2341 cagcgcagaa gtgcgcctgg ctcgacgacg cgacctatca gctcgaggtg cctttcgtca
     2401 acgagacaga gctgctgatg gacgtcctgc ggcatgggga ccaggtgtcc gtggtttcgc
     2461 cgccttcgct ggtcgcgctg atcgctcatc gactcgaatc ggcatcgcag cattacaagg
     2521 gacgcgcatg aaaccctttg ccgatgtact gatgcgttcg atccatcggg tgcttgacca
     2581 cgatgcgttg ggcgctctcc gatgacgacg cagtctcctg ttgcctcaaa atttcctttg
     2641 cttctgcccc ttcatcaagg gtgagcccaa ttcaagagat cacatggact cctacctacc
     2701 cacgatcctt caactccgtt tggtggtcgg atttctcggc gagcgagccc agcacggctg
     2761 gtggccgacc agcttcttcg gcgactacag catgcgatcg cttgagtttg tcgcgcccaa
     2821 gacggcgacg ctggcgcagt accacggggc cgttgaggcc gctcgccggc tgcatgatga
     2881 acacctcagc gttggcagct accacctgtt ccgcctgcct gaagaggtgg agcaggatct
     2941 gcacgcattg gtgcaaagct cccagggcgc cgaggtcatc agccagctgc cgaaggacaa
     3001 ggagagtgcc ctgtccagcc tggccaaaat ggccaacgcg ggggttacag ctgcggaagg
     3061 tcctgtctca gtggggtcga tcagtgccat gagcgaaccg gacaccgcga aggcaattgc
     3121 cggcgtctac gtgaaggccc tgtccgccaa cgtcaaagcc tacccgtact tggtgaagtg
     3181 acgttggcgc tccctccata caccacccag ctgcaggctg gcctcggctt ggtcgacgag
     3241 accaagactt tgctcgacat ttggtcacct gggatgacac cgccacagct gcaggcggca
     3301 tcgttggcgt ctggccgatt caccgcagtg accgcccggc gattgcgcaa cgtggtcatc
     3361 gaatgcttca agcctcgcta cctcgtcgcc ggcggcgccc ccgcggcgca cttgaagcgg
     3421 ctctcaacgg tgctgactgc cgccgaactg gcgcaggcca tgatgctgtt cacggcccga
     3481 gcgaacccca tcctggctga cttcatccga caggtgtact ggtcgcgcta cgcgggcagc
     3541 tacacccacg tcacgaacga cgatgcccgc gccttcgtcg aacgcgccat cgacgatggc
     3601 cggacggcca cgcgctggtc gcaaaccacc atcccacgcg tttcgcggta cctcacgggc
     3661 tgctgtgccg actatggcct gcttgagcgg ggccaacgct ccagccgccg catcctgcct
     3721 tttcggatcg cctttcccgt cgcggcctac ctggcctacg acctccactt cgccggcgtc
     3781 ggcgacaacg cattgctcgg ccacgaggac tggatgttgt tcggcctgga ccggcaagcg
     3841 gtgctgagcg agctcaaaca gctttcgttg aagggcctgt tgatcgtcca ggctgccggc
     3901 gacgtcgtcc ggatcagttg gaaacaccaa atcatggagt ccttgctcga tgtcctcact
     3961 caaagctgac ttcgacgagc tgcgcgagcg catccggcat ggccgcgacc tgggcccggc
     4021 aagcttcgag cccgtcttct acctcgtctt cccgccggag cagatccttg aggtcaagcg
     4081 ccagatgccg gcgtggatct cgaagcttca tcaagaaggg tgggacgtgc acaccttttc
     4141 gatggccgag cacatctggt cggtcctgaa ggcggaccca ctgtggcccc tgtgcgccat
     4201 ggaagaccgt tccgccccat tggattgggc ccgcactaac aaggccctgg cggacatcct
     4261 ggtcggtgaa ggcgccttgc tcaagcgctt ggagagcgcc ctggaaccgc tcgaaggcaa
     4321 gcacaacgcc gtcatgctcg tcaccgacct ggaagccttg catcccttcc ttcgcatcgg
     4381 cgccatcgaa agccagttga tgggcaagtt tcatgtgccc accgtcttct tctaccccgg
     4441 cattcgcacc ggcaagacga acctcaagtt cctgggcttc tacccagcgg atggcaacta
     4501 ccggtccgtc cacgtcggcg gctgagcccg cacaagtctc cagggaaaaa caatgaacat
     4561 tcgtacgctc ttcgacccga gcaaagacat cttccgcaac atcgagaagg tcatcactta
     4621 cggtgcgtcg caggagagcc gcctcaagtc cgagatctcg gaatacgtcg tcaccgagag
     4681 catcgaagag cagttccgca agctgctgga tcgcatgcag ctggccatgg aatcgggtgg
     4741 cgagaacgaa gttggcgtgt gggtg
//
LOCUS       DAOORV010000244         4692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21783_length_4692_cov_11.794264, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000244 DAOORV010000000
VERSION     DAOORV010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21783_length_4692_cov_11.794264"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..829)
                     /locus_tag="PKV56_18290"
     CDS             complement(203..829)
                     /locus_tag="PKV56_18290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein A"
                     /protein_id="HOX69801.1"
                     /translation="MTTDLRDAAIGVSCPVVAVPRFGALEPHASGQRMLLARNGLFVQ
                     MKTPWLDCTTRVAEIGMHLPYGAAAEAITFAFGVIPLGLLERFIAAARGTMPNEAAGA
                     LVYDARSGALRLAMHEAIEVGPGHVRYRIEELAADELVAIDLHSHGRLAAFWSGEDDR
                     DDQGVRVCGVFGNLDRERPTARFRLALNGLFRELPHPWAVEPDGALPA"
     gene            complement(819..1574)
                     /locus_tag="PKV56_18295"
     CDS             complement(819..1574)
                     /locus_tag="PKV56_18295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein B"
                     /protein_id="HOX69802.1"
                     /translation="MNAHERDVKFNVSEEGPAEMHLRRAILVYGGKGGGQFATVHDIA
                     TAPGGGATILPGRPMTAFAVQRLARKLTKRREGGFIPEKLIFQDADTIAWWVPPSRRR
                     VWFRCPEGQLIAGERSEVVSHPGLVFSVATARKWFVWAIKGTARPTDSTKLFRAPYFN
                     VWESGQVCVGNVDLPERASAEKLDEWTNAFFESWFTHPNVHAELVRYRGGAYRFWRDM
                     LDGKHVVFPERTLVDLDRNLGEALQSRGAKHDN"
     gene            complement(1595..2593)
                     /locus_tag="PKV56_18300"
     CDS             complement(1595..2593)
                     /locus_tag="PKV56_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HOX69803.1"
                     /translation="MFQLPSIDPSIPCTLVPQASRRRGALLARTLIRAGAFDAALMPS
                     RPGPDHIKTCQTVLEGWVRRELGALRMLRPYFGMHLRETGNDASQAVGPACLIEWGGE
                     SFGVRCVGPALECLERHRPRLGRTVLRALERYAFRTLPIYTPAVVMECASDVYWYGEC
                     DEEMALEEACGDDAEERKSMRDSMVTRAMFDSTFPCWALEGRTRLLGRRTLLQIAESA
                     SDRRIASVIESVLGLHAMNPPGYDWTFREGRFVGYSGVLAWGREDELTCRVLDDYEQM
                     VSESGDYFETCGEVPIGVEDHEALTGWFEYMRSWCRATHGLDTLIWQLSEGDWSVK"
     gene            complement(2816..3022)
                     /locus_tag="PKV56_18305"
     CDS             complement(2816..3022)
                     /locus_tag="PKV56_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein C"
                     /protein_id="HOX69804.1"
                     /translation="MSIVVAALARCFKYNGMTLPDIPGLSVEQVKEVYAAQYPELLTA
                     EAQVGEVTRGVQEIAFKRAVGTKG"
     gene            complement(3035..3529)
                     /locus_tag="PKV56_18310"
     CDS             complement(3035..3529)
                     /locus_tag="PKV56_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein E"
                     /protein_id="HOX69805.1"
                     /translation="MFKELFPLAAKGSFSLLLSADEGSGLMTVVVVPKHSDGGKDVPA
                     LAMPLKLTAAPEELDAGFTQAVLSYSTHRASLDEQVAATNEVIEAAKAASVKKGAEAV
                     TKAGAKPTQRAAAPKAAAGDDDADDDHGGDGTDGAPTAMHGVAPATAEAPSAGPGVPD
                     LFGA"
     gene            complement(3602..>4692)
                     /locus_tag="PKV56_18315"
     CDS             complement(3602..>4692)
                     /locus_tag="PKV56_18315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRTRC system ParB family protein"
                     /protein_id="HOX69806.1"
                     /translation="YCQHPTHYDELTMAALEARAEPLRERFQVIRFVKPEDGFVPLAV
                     AADGPLGVGTEQMEACKGCANFGCSVSAIPGHYGEVAESLCFDAACNSTKVAARRKAE
                     RAASKPTSSARDAAGVGDSKTKSAEPAASAPESAKPRNTVPGKVVAHRIEFWRKTVAN
                     QLMVQGERNHRALTALVLARETRAIHADRYADVAVKLAGIKKPNLGETPLRKMLEQAD
                     AVNASALEALVKAVTASAAYGVDTEGLETLLNYLEVDEARHFTLNEDYLALLTVSELE
                     SLAEEVGLKKAMGERYAKAKAGKRGEFIQALLAVEGFTYKGTVPKAMRYPRRKFRYAE
                     RADAAGEPSAATGGPGVRREVAEPAHGA"
BASE COUNT          744 a         1675 c         1434 g          839 t
ORIGIN      
        1 cctctcccga agatgatcgt ggaccaggcg tccgcttcaa ccccggcgcg cgcgcgccat
       61 cggcaagctc gtcggcggtg gccgctcagc gacgagccgc agtgcacaat gaggagcgca
      121 ttcgatccgt ccttgcttgt cctccctggg caaggcctga tgcgtcttcg gcgcctcggc
      181 gttcagcgtc gaggcgcctc ttctatgccg gcagcgcgcc gtccggctcg acggcccacg
      241 gatgaggcaa ctcccggaac agtccgttca gcgcgagccg gaacctcgcg gtgggtcgct
      301 cacggtccag gttgccgaag acaccgcaga cccgcacgcc ctggtcatcc cgatcgtctt
      361 cgcccgacca gaacgcagcc agccggccat gtgagtgaag gtcgattgcg acaagctcgt
      421 ctgcggccaa ctcttcgatc cggtacctca cgtgaccggg cccgacctcg atggcctcat
      481 gcatggcgag gcgcaaagca ccgctgcgcg cgtcatagac cagcgcgccc gccgcctcgt
      541 tgggcatcgt cccgcgcgcg gcggcgatga accgctccag caatcccaag ggaatgacgc
      601 cgaaggcgaa cgtgatggcc tcggccgccg ccccgtacgg caggtgcatg ccgatctccg
      661 cgacccgcgt cgtgcaatcg agccagggcg tcttcatctg cacgaacagg ccgtttcggg
      721 cgagcagcat gcgctgccca ctcgcgtgcg gctccagtgc gccgaatcga ggcacggcga
      781 ccaccgggca actgaccccg atggccgcgt cgcgcaggtc agttgtcatg cttggctccg
      841 cgggactgca aggcctcacc taggttgcgg tcgaggtcca ccaaggtccg ctccggaaac
      901 acgacatgct tgccgtcgag catgtctctc cagaacctgt aggcgccacc tcggtaccga
      961 acaagctcgg cgtgcacgtt ggggtgggtg aaccagcttt cgaagaaggc gttggtccac
     1021 tcatccaact tctcggccga ggcccgttca ggcaggtcca cgttgccaac acacacctgg
     1081 ccgctctccc agacgttgaa gtaaggtgcg cgaaacagct tcgtgctgtc ggtgggccgc
     1141 gccgtgccct tgatcgccca gacaaaccac ttgcgcgccg tcgccacgct gaacaccagc
     1201 cccggatggc tgacgacctc ggaccgctcc cccgcgatga gttggccctc gggacatctg
     1261 aaccacacgc gtcgcctcga cggaggcacc caccatgcga tcgtgtcagc gtcctggaag
     1321 atcagcttct cagggatgaa tccgccttcg cgccgcttgg tcaacttgcg ggctagacgc
     1381 tggactgcga atgccgtcat cggccgtccc ggcaggatcg tcgccccacc gcccggagcc
     1441 gtcgcgatgt catggaccgt cgcgaactgc cccccgccct tgcctccgta caccaagatc
     1501 gctcgacgca gatgcatttc agccggtcct tcctcggaca cgttgaactt cacgtctcgt
     1561 tcatgtgcgt tcatcgagtt cgttcctttc acgatcactt cacggaccag tctccctcgc
     1621 tgagttgcca gatcaaggtg tcgaggccat gcgtcgcacg gcaccacgac cgcatgtact
     1681 cgaaccagcc ggtgagtgcc tcgtgatcct cgacaccgat cggaacttcg ccgcaggtct
     1741 cgaagtagtc gccgctctcg ctcaccatct gctcgtagtc atcgagcacg cggcacgtga
     1801 gttcgtcctc acggccccag gccaggacgc ccgagtagcc aacgaagcgt ccctcccgga
     1861 aagtccagtc gtagccgggc gggttcatcg catgcaggcc cagcacgctt tcgatcacac
     1921 tggcaatgcg acggtccgac gcggactctg cgatctgcag caatgtcctc cggccgagca
     1981 gcctcgttcg gccttccagc gcccagcacg ggaacgtgct gtcgaacatg gcccgggtca
     2041 ccatgctgtc gcgcatcgac ttgcgctcct cggcatcgtc gccacaggct tcttccaggg
     2101 ccatttcctc gtcgcactcg ccgtaccagt agacgtcgct ggcgcactcc atgacgacag
     2161 cgggcgtgta gatcggcagc gtgcgaaacg cataccgctc cagagcacgc aacaccgtcc
     2221 ggccgagcct gggacggtga cgttccaagc actccagcgc aggccccacg cagcggacgc
     2281 cgaaggactc gccaccccac tcgatcaggc acgctggccc gaccgcctgg ctggcgtcgt
     2341 tccccgtctc gcgcaggtgc atcccgaagt acggccggag catccgcaac gcgccgagtt
     2401 ctcggcgaac ccaaccctcc agcaccgtct ggcaggtctt gatgtggtcg ggacccggcc
     2461 gggacggcat caaggctgcg tcgaaggcgc ctgcgcgaat caatgttcga gccagcagcg
     2521 caccacggcg ccgcgacgcc tgcggcacca aagtgcaggg aatcgatggg tcgatggagg
     2581 gcaactgaaa catggctaga acagtggcgg atagagcgta ccccgtacca ccatgcgctc
     2641 ctgccgctcc ccgtggggct ggccccgcac gtcttctgcg aactggtgga cgatgctgct
     2701 cacgagaacc agtttcgcct cgcccatcgc ggcttcaagg gccccggcat cgccctgtcc
     2761 gggctgcgcg aggaaccgat ccagccgcgc ggcgtagcac gcgaccggat cgtcctcagc
     2821 ccttggtgcc gaccgcgcgc ttgaacgcga tttcctggac gccacgggtg acctcaccga
     2881 cctgggcctc ggccgtcagc agttccgggt actgcgcggc gtagacctcc ttgacctgtt
     2941 cgacgctgag gccgggaatg tccggcagcg tcatgccgtt gtacttgaag caacgggcga
     3001 gagcggcaac gacgatggac atggttgcct cccttcacgc gccgaagaga tccggcacgc
     3061 ccggtcccgc agagggagcc tccgccgtag ccggtgcgac cccgtgcatc gctgtgggcg
     3121 caccgtcggt gccgtcccca ccatggtcgt cgtctgcgtc gtcgtcacct gccgctgcct
     3181 tgggtgctgc ggcccgttgc gtgggcttgg cgccggcctt cgtcacggcc tcggcgccct
     3241 tcttgaccga cgcggccttc gcagcctcga tgacttcgtt ggtcgcggcc acttgttcgt
     3301 ccaggctggc gcgatgggtc gagtagctga gcacggcctg cgtgaagcct gcatccagtt
     3361 cttcgggcgc ggccgtcagc ttgagcggca tcgccagtgc aggaacatcc ttgccgccgt
     3421 ctgagtgctt cggcacgacc accaccgtca tgaggccgct gccctcgtcg gctgaaagca
     3481 gcaagctgaa cgaacctttt gctgccagcg ggaatagctc tttgaacatc gcttcaatct
     3541 ccttgatcga gagggatgac cctcggatcg atcctatggg tcacccctgg tttgtcgagg
     3601 ctcacgctcc gtgggcgggc tcagcgactt ctcggcgcac gcccggccca ccggtggccg
     3661 cgctgggctc accggctgca tcggcgcgct cggcgtacct gaacttgcgc cgggggtagc
     3721 gcatggcttt gggcactgtg cccttgtagg tgaagccctc gaccgccagc agcgcctgga
     3781 tgaattcgcc tcgcttgccg gccttggcct tcgcgtagcg ctcgcccatc gccttcttca
     3841 ggccgacttc ctccgccagc gactcaagtt cgctcaccgt cagcagtgcc aggtagtctt
     3901 cgttgagcgt gaagtgccgt gcctcgtcca cttccaggta gttcagcagc gtttcgagcc
     3961 cttcggtgtc gacgccatag gcggccgacg ccgtgaccgc tttgacaagg gcctccagcg
     4021 cgcttgcgtt caccgcgtct gcctgctcca gcatcttgcg cagcggcgtt tcgcccaagt
     4081 tcggcttctt gatgccggcc agcttgaccg ccacatcggc ataccgatcc gcatggatgg
     4141 ctcgcgtctc acgagccaac accagggccg tcagtgcccg gtggttgcgc tcaccctgca
     4201 ccatcagctg gttagccacc gtcttgcgcc aaaactcgat gcggtgcgcc actaccttgc
     4261 ccggcaccgt gttgcgcggc ttggcgcttt cgggggccga ggccgctggc tcggcactct
     4321 tggtcttgct gtcgccaacc ccagccgcgt ccctcgccga cgaagtcggc ttgctcgctg
     4381 ctcgctcggc cttgcgccgc gcggccacct tggtgctgtt gcacgcggcg tcgaagcaca
     4441 gcgactcggc gacctcgccg tagtgacccg ggatggccga cacgctgcag ccgaagttcg
     4501 cgcagccctt gcaggcctcc atctgctccg tgcccacacc aagggggcca tccgccgcta
     4561 ccgcgagagg cacgaagcca tcttccggct tgacgaaacg gatcacctgg aagcgttcgc
     4621 gcagcggctc ggcccgggcc tccagcgccg ccatcgtgag ttcgtcgtag tgggtcgggt
     4681 gctggcagta gc
//
LOCUS       DAOORV010000245         4672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_21890_length_4672_cov_9.930691, whole genome shotgun sequence.
ACCESSION   DAOORV010000245 DAOORV010000000
VERSION     DAOORV010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21890_length_4672_cov_9.930691"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKV56_18320"
     CDS             <1..739
                     /locus_tag="PKV56_18320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOX69807.1"
                     /translation="SKVRNAKVMSALNDNRHGTLLDFIDDFTKRFPKCVDDYETLLTD
                     NRIWKQRTVGIGVVTPERALNLGLTGAMLRGSGVEWDLRKKQPYDVYEHMDFDIPVGV
                     NGDTYDRYLVRVEEMRQSNRIIQQCVAWLRANPGPVITSNHKVAPPSRVDMKSSMEDL
                     IHHFKLFTEGFHVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRAPGFAHLAALD
                     EMARGHMIADAVAVIGTMDIVFGEIDR"
     gene            736..1245
                     /locus_tag="PKV56_18325"
     CDS             736..1245
                     /locus_tag="PKV56_18325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOX69808.1"
                     /translation="MSAPIQFSDATLERFAREVAKYPAEQKQSAVMACLAIVQQELGY
                     VSAESENFVADYLGMAPIAVHEVTTFYNMYNQQPLGRYKINVCTNLPCQLRDGQDALE
                     HLCRRLGVEEGGTTSDGLITLQKSECLGACADSPVLLVNDRQMCSFMTHERLDALVAV
                     LKSQDDSSR"
     gene            1264..2607
                     /gene="nuoF"
                     /locus_tag="PKV56_18330"
     CDS             1264..2607
                     /gene="nuoF"
                     /locus_tag="PKV56_18330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOX69809.1"
                     /translation="MLDLSQFHADGVATCFHGRHIGAQIYADLNGRNWGLADYEARGG
                     YQALRKILGLDGGEGMTPDQVIAEVKTSGLRGRGGAGFPTGLKWSFMPRMYPGNKYLV
                     CNSDEGEPGTCKDRDILMFNPHIVIEGMAIAAYAMGIKTGYNYIHGEIFEVYQRFEEA
                     LEEARAAGFLGDNILGSGFSFQLHGFHGFGAYICGEETALLESLEGKKGQPRFKPPFP
                     ASFGLYGKPTTINNTETFAAVPWIIRNGGPAYLECGKPNNGGTKIFSMVGDVERPGNY
                     EIPLGTPFSKILELAGGVKGGRKLKAVIPGGSSAPVLPAATMMDCTMDYDSIAKAGSM
                     LGSGAVIVMDETRCMVKSLLRLSYFYSHECCGQCTPCREGTGWLWRLVNRLEHGQGKA
                     EDLALLDSVAESIMGRTICALGDAAAMPVRGMLKHFRDEFAYHVDHKTCMVPQYV"
     gene            2629..>4672
                     /gene="nuoG"
                     /locus_tag="PKV56_18335"
     CDS             2629..>4672
                     /gene="nuoG"
                     /locus_tag="PKV56_18335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOX69810.1"
                     /translation="MIEIELDGKKVSVAEGSMVMHAADAAGTTIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPMPACATPVTQGMIVRTKSPRALQAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASSTRYHEEKRVVFHKEVGPLISMEEMSRCIHCTRCVRFGQEV
                     AGVMELGMIHRGEHSEITTVAGDTIDSELSGNMIDVCPVGALTSKPFRYSARTWELSR
                     RKSVSPHDSTGSNLIVQVKSGKVMRVVPLENEDVNECWIADRDRFSYEAVNSEQRLTT
                     PLIKQGGEWKPVDWTTALHYVANGLAQIKADHGAASIGALGSAHSTVEELHLLAKLVR
                     GLGSDNIDHRLRQSDFSATRSAGSARWLGMPIAALSSLQRVLVVGSFLRKDHPLFAQR
                     LRQAARKGARIHSLQAVHDDWLIPLAGTITAAPSAWVQSLAEVAAAVAASKQVPEPVA
                     LGAGVEPSAEAKAIAASLLGGERKAILLGNAAVQHPAASSLMALAQWIGEHTGAAVGD
                     LTAAANSVGAQIVGAQPAAGGLNAAQMLGGSLKACVLLNVEPDRDAANPAAAVGALQG
                     AEMVVALTSFRDAALDCADVLLPISPFTETSGSFINAEGRLQSFHGVVKPLGDTRPAW
                     KVLRVLGNMLNVPGFDFETSEDVRAEAVGDGSGISARLSNRIDTPASLTPVATGFER"
BASE COUNT          871 a         1492 c         1493 g          816 t
ORIGIN      
        1 ttcgaaggtg cgcaacgcga aggtgatgtc ggcattgaac gacaaccgtc acggcacgtt
       61 gctcgacttc atcgacgatt tcaccaagcg ctttcccaag tgcgtcgacg actacgaaac
      121 cctgcttacc gacaaccgga tctggaagca gcgcaccgtc ggcatcggcg tggtgacccc
      181 ggagcgtgcc ttgaacctgg gcctgaccgg tgcgatgctg cgcggctccg gcgtcgagtg
      241 ggaccttcgc aagaagcaac cgtacgacgt ctacgagcac atggatttcg acattcctgt
      301 cggagtcaat ggagacacct acgaccgcta cctggtgcgc gtcgaggaga tgcgtcagtc
      361 caaccggatc atccagcagt gcgtcgcctg gttgcgcgcg aatcctgggc cggtcatcac
      421 gtccaaccac aaggtcgcgc cgccttcccg tgtcgacatg aagtcgagca tggaagacct
      481 gattcaccac ttcaagctgt tcaccgaagg cttccacgtc cccgagggcg aggcctacgc
      541 ggcggtcgag catccgaagg gtgagttcgg catctacatc gtcagcgacg gtgccaacaa
      601 gccataccgg ctcaagatac gcgcacccgg ttttgcgcat ctcgcggccc tggacgaaat
      661 ggctcggggc cacatgattg ccgacgcggt tgcggtcatc ggcacgatgg acatcgtttt
      721 cggagagatc gaccggtgag cgcgcccatt cagttttccg atgccactct cgagcgcttt
      781 gcgcgcgagg tggccaagta ccccgccgag cagaagcagt cggccgtgat ggcctgcctt
      841 gcgatcgtgc agcaggaact cggctacgtg tccgccgaga gcgagaactt cgtggctgat
      901 tacctcggca tggcgccgat tgccgttcac gaggtcacaa cgttctacaa catgtacaac
      961 cagcagccgc tggggcgcta caagatcaat gtctgcacca acctgccttg ccagttgcgc
     1021 gatgggcagg atgcgctgga gcacctgtgc cgtcgtctcg gggtcgagga ggggggcacc
     1081 acgtctgatg ggctgatcac gctgcagaaa agcgaatgtc tgggcgcctg cgccgactcg
     1141 cccgtgctgc tcgtcaacga caggcagatg tgcagtttca tgacccacga gcggctggat
     1201 gcgctggtgg ctgttctcaa gtcgcaagac gactcgtccc gctaagcagt ccaggggcct
     1261 ttcatgctcg atctctccca atttcatgcc gacggtgtgg ccacctgctt ccacggccgt
     1321 cacatcggcg cgcagatcta tgcggacctg aacggccgca attggggtct ggccgactat
     1381 gaagcgcgcg gcggctatca ggcgctgcgc aagatcctgg gcctcgatgg gggcgagggg
     1441 atgactcccg atcaggtgat cgccgaggtc aagacctccg gtttgcgcgg gcgagggggc
     1501 gctggtttcc cgacgggcct gaagtggagc ttcatgccgc gcatgtaccc gggcaacaag
     1561 tacctggttt gcaactcgga cgaaggcgaa ccgggcacct gcaaagaccg cgacatcctg
     1621 atgttcaacc cgcacatcgt catcgaaggc atggccatcg ccgcctacgc gatgggcatc
     1681 aagacgggct acaactacat ccacggcgag atcttcgagg tctaccagcg cttcgaggaa
     1741 gcgctggaag aagcccgtgc cgccggcttc ctgggtgaca acatcctggg cagcggcttc
     1801 agcttccagt tgcacggctt ccatggcttc ggcgcctaca tctgcggtga agaaacggcg
     1861 ctactggaat cgctggaagg caagaagggc cagccgcgct tcaagccgcc gttcccggcc
     1921 agcttcggcc tgtacggcaa gccgaccacg atcaacaaca ccgagacctt cgccgcggtg
     1981 ccctggatca tccgcaacgg cggcccggcc tacctggaat gcggcaagcc gaacaacggc
     2041 ggcaccaaga tcttctcgat ggtcggtgat gtcgagcggc cgggcaacta cgagatcccg
     2101 ctgggcacgc cgttcagcaa gatcctggag ctggccggtg gcgtcaaggg tggcaggaag
     2161 ctcaaggcgg tgatcccggg cggatcgtcg gccccggtgc tgccggcggc caccatgatg
     2221 gactgcacca tggactacga cagcatcgcc aaggccggct cgatgctggg ctcgggcgcg
     2281 gtgatcgtga tggacgagac ccgctgcatg gtcaagagcc tgctgcgcct gtcctacttc
     2341 tacagccacg agtgctgcgg ccagtgcacg ccctgccgcg aaggcacggg ctggctgtgg
     2401 cgcctggtca accggctgga acacggccag ggcaaggccg aggatctggc cctgctcgac
     2461 agcgtggccg aaagcatcat gggccgcacc atctgcgcgc tgggcgacgc ggcggccatg
     2521 ccggtgcgcg gcatgctcaa gcacttccgc gacgagtttg cctaccacgt cgaccacaag
     2581 acctgcatgg tcccccaata cgtctgatcg gcggagccga gcaacgacat gatcgaaatc
     2641 gaactcgacg gcaagaaggt cagcgtcgcc gaaggcagca tggtgatgca tgcggccgac
     2701 gcggccggca ccaccatccc gcacttctgc taccacaaga agctcagcat cgcggccaac
     2761 tgccgcatgt gcctggtcga cgtcgagaag gcacccaagc cgatgccggc ctgcgcgacg
     2821 ccggtcacgc agggcatgat cgtgcgcacc aagagcccgc gcgcgctcca ggcccagaag
     2881 ggcgtgatgg agttcctgct gatcaaccac ccgctcgact gcccgatctg cgaccagggc
     2941 ggcgagtgcc agctgcagga tctggctgtc ggctacgggg cttcgagcac gcgctaccac
     3001 gaagagaagc gcgtcgtctt tcacaaggaa gtcggcccgc tgatttccat ggaggagatg
     3061 agccgctgca tccactgcac ccgctgtgtc cgtttcggcc aggaagtcgc aggggtgatg
     3121 gaactcggca tgattcatcg cggtgagcac tccgagatca ccaccgtcgc tggtgacacc
     3181 atcgactccg aactgtcggg caacatgatc gacgtctgcc cggtcggtgc gctgaccagc
     3241 aagccgttcc gctacagcgc ccgtacctgg gagctctcgc gtcgcaagtc ggtttcgcca
     3301 cacgacagca caggcagcaa cctgatcgtt caggtcaagt ccggcaaggt catgcgtgtc
     3361 gtccctctcg agaacgaaga cgtcaacgaa tgctggatcg cagatcgtga ccgcttttcg
     3421 tacgaggcag tcaacagcga gcagcgtttg accactccgc tgatcaagca gggcggcgaa
     3481 tggaagccgg tcgactggac cacggcattg cattacgtgg ccaacggtct ggcgcagatc
     3541 aaggccgatc atggtgccgc atccatcggt gcgctcgggt ctgctcacag cacggtcgag
     3601 gaacttcact tgctggccaa gctggttcgt gggctgggca gcgacaacat cgaccaccgt
     3661 ctgcgtcagt ctgacttctc ggccacccgc agcgctggtt ctgcccgctg gttgggcatg
     3721 ccgatcgcgg cgctttccag cctgcagcgg gtgctcgtgg tcggatcctt cctgcgcaag
     3781 gaccatccgc tgttcgcgca gcgtctgcgt caggccgccc gcaagggggc acgcatccac
     3841 agcctgcagg ctgtgcatga cgactggttg atcccgttgg ctggcacgat cacggctgcg
     3901 ccgagtgcct gggtgcagtc cctggccgag gtggctgcag cggttgcggc atccaagcaa
     3961 gtcccggagc cggttgcact gggcgccggt gtcgagccct cggccgaagc gaaagccatc
     4021 gccgcttccc tgctgggtgg agagcgcaag gccatcctgc ttggcaacgc ggcagtgcag
     4081 catccagcgg cctccagcct gatggcgctg gctcaatgga tcggcgagca caccggtgcg
     4141 gcggtgggtg acctgactgc ggcggccaat tccgttggcg cacagatcgt cggcgcccag
     4201 cccgctgccg gtggactgaa cgcggcgcaa atgcttggcg gttcgctcaa ggcgtgcgtg
     4261 ctgctcaatg tcgagcccga tcgcgatgcc gccaacccgg cggccgccgt gggcgcgctg
     4321 caaggcgcag aaatggtcgt tgcactgact tctttccgcg atgcggctct cgattgtgcc
     4381 gatgtgctgc ttccgatctc gcccttcacc gaaacatcag gcagtttcat caatgccgaa
     4441 gggcgcttgc agagcttcca tggcgtggtc aagcctctgg gggacacccg tccggcctgg
     4501 aaggtgcttc gcgtgctggg caacatgctc aacgtaccgg gcttcgattt cgagacctcc
     4561 gaggacgtca gagccgaggc cgtcggcgac ggatcgggca tcagtgcccg cttgagcaac
     4621 aggatcgaca caccagcctc gctgacgcct gtggcgaccg gtttcgaacg ca
//
LOCUS       DAOORV010000246         4624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_22183_length_4624_cov_7.392208, whole genome shotgun sequence.
ACCESSION   DAOORV010000246 DAOORV010000000
VERSION     DAOORV010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22183_length_4624_cov_7.392208"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKV56_18340"
     CDS             complement(<1..229)
                     /locus_tag="PKV56_18340"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX69811.1"
                     /translation="MLTERYADALQLAWKLHSTQVRKGTTIPYISHLIAVSSLALEHG
                     ANEDEAIAALLHDAVEKWPRLSEQHAPIFKWT"
     gene            complement(219..596)
                     /locus_tag="PKV56_18345"
     CDS             complement(219..596)
                     /locus_tag="PKV56_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69812.1"
                     /translation="MLKSLEGCYKHGLADVVTEGAGVVSPLPELQERKRTMAASPARL
                     IAAARHLKRMQDHCQWEIDQGVPLEQRIPDDELAARTMDPADALITSLLLNREFKVEN
                     PDHPESLADRAIEQAKRRSRRAD"
     gene            complement(803..1816)
                     /locus_tag="PKV56_18350"
     CDS             complement(803..1816)
                     /locus_tag="PKV56_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69813.1"
                     /translation="MIQLMAVPASLLAHVGTGAAYVSGALVKDSASHQILAHLQPTSR
                     LSQWVFDVGMAGPLAPLEVASSLGANAQLLEIRGMLETLQTVASVGAAASVLNLGVSI
                     GGFAMVLTSLRRMEGTMDKVFAAVKSIENVQTADYLGTTMAAIERAESAFALPSPGDR
                     RRYWEEADLELSRAVGIALKRLAARGVGLDDGSQKPDADPETARRLCTPEAVELLRWL
                     LALSSTRVEVLLGLQMPTEAARQSRETARWMTSLPFDSMDLAKAQLANRTVPPTQMAR
                     ITSQASALSLLIERAGAVSGERAMLCDHLHEQGADSLEYVLTVRNHPEATVLMLPHSA
                     SIL"
     gene            complement(1813..2640)
                     /locus_tag="PKV56_18355"
     CDS             complement(1813..2640)
                     /locus_tag="PKV56_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69814.1"
                     /translation="MPYDDRLFALLRELDDQELRTIWVDALKCAPDRDDFAIGSRELK
                     ILNISKEWRAVHGHTLLNLRRGNHELPWRRILVDVADKLKPGWGWSDFRTDDERSDGE
                     IEAAILRYFDERAQEAWAKMNEEERAKLASSLEQELAQAQANVGASVRGAGISGITTS
                     SLGAGIGAGLLSGAGALSLAQGATSFALSGLIGGTLYQLSFWIVVRVFGAWSGVQLMA
                     SGGLATVAGALLSVPALVAFAANAVMSTSYRKTIPATLALLTAHELRRQLADLEASK"
     gene            3162..3374
                     /locus_tag="PKV56_18360"
     CDS             3162..3374
                     /locus_tag="PKV56_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69815.1"
                     /translation="MFLAFILLIALALVFVKLGALSVWVTVLSASLQTLLALLAAVAA
                     AVGWKAWRRRRDGISFTEARVLHKRD"
     gene            3412..4323
                     /locus_tag="PKV56_18365"
     CDS             3412..4323
                     /locus_tag="PKV56_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69816.1"
                     /translation="MPAPSIAVSGLPSFGDFAASAIVSAIKALYRAAGQFNDWLDEHI
                     ADLKRSDDPLIASIGRVLEGAKFGFGMGYVASTALIAVGQFLLGNTFAAVATVASAAA
                     LSNPIAMTCAAIGAIYFGWKALTDKEREQILEHLAAGLTLGVELIRAVVEFAIRRSRE
                     LLDSSQLEAAKTFVKAQAEAFGRTLYDVTKQLGDLASGSAEAIAEKAGQAAVAIKGAA
                     GKVGDAVAGGASSAQEAIGTGVARLRELATDAGKSLKRASGPPVLTTVPWESGAAVDN
                     APVLPGAPDHQLGPAHKTGATGSDPQQ"
     gene            4320..>4624
                     /locus_tag="PKV56_18370"
     CDS             4320..>4624
                     /locus_tag="PKV56_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOX69817.1"
                     /translation="MTSGNHDTLVHRLSLMLLKLNQGEALDPRALAEEFGVNLRTIQR
                     DLNRRFTYLPLHKSEGRYRLDPAFLGQFTAKDVQAFAALAGVQELFPHLTDEFLRDV"
BASE COUNT          864 a         1489 c         1406 g          865 t
ORIGIN      
        1 cagtccactt aaaaatcggg gcgtgctgtt cagacaagcg gggccacttc tctactgcgt
       61 cgtgcagcag cgcggcgatg gcttcgtcct cgtttgcgcc atgttcgagc gcaaggcttg
      121 aaacggcaat gaggtgagag atatacggga tcgtggtccc cttgcgaacc tgggtgctgt
      181 gcaacttcca ggcaagttgc aacgcgtctg cgtagcgttc agtcagcacg gcgcgacctc
      241 ctctttgctt gctcgatggc tcggtcggcg aggctctccg gatggtcggg gttctcgacc
      301 ttgaactcgc gattgagcag taggctcgtg atgagcgcgt ccgccggatc catcgtcctc
      361 gcggcaagtt cgtcgtcggg aatgcgctgt tcaagtggaa cgccttgatc aatctcccac
      421 tgacagtggt cttgcattct cttgaggtgt ctcgccgcgg cgatcaggcg tgctggactt
      481 gctgccattg ttcgtttcct ctcctgcaac tccggcaaag gcgaaaccac cccggcgcct
      541 tcagtaacga catcagcgag gccatgcttg tagcaacctt ctagactctt cagcaccgag
      601 cattcgctca atgatgaaca gtgtagccac cggctagctc aaaacatgag cgagaaggcc
      661 tcgcaagtgg aaccgggatc gccagattgc ccaggctgtc gcgcgccaga ttagaggtgg
      721 aaagaggttt gcggttcgat cccttctgtt gcgaggccgc ccgctagctc gggccggccg
      781 cagggaacaa gggctggccc gctcagagaa tgcttgcgct atggggcagc atcagaacgg
      841 tagcctcggg atgattccta accgtaagca catactcgag actgtcagcc ccttgctcgt
      901 gcagatggtc acacagcatc gctcgctctc ccgaaactgc acccgcacgc tctatcagca
      961 aactcagcgc gctcgcctgc gaggttattc gggccatttg ggtgggcgga accgtacggt
     1021 ttgctagttg cgccttggcc agatccatgg agtcgaaggg cagagatgtc atccaacgcg
     1081 cggtttcccg cgactgcctt gccgcctctg tcggcatctg cagacccaac aacacttcga
     1141 cgcgcgtact ggaaagagcc agcagccagc gaagcagctc cactgcttcg ggcgtgcaca
     1201 accggcgcgc ggtttctgga tctgcgtcag gcttctggga tccgtcgtcg agcccgacgc
     1261 cgcgcgccgc aagacgcttc agggcgatgc cgacggctcg gctcagctcc aaatctgcct
     1321 cttcccagta tcgccggcgg tcacccggcg acgggagcgc gaacgcactt tcggctcgtt
     1381 cgatggccgc catcgtcgtg cccagatagt cggctgtctg gacgttctca atgctcttca
     1441 ccgccgcaaa gaccttgtcc atggtgcctt ccatccggcg aaggctagtc agcaccatgg
     1501 cgaagccacc gatcgatacg cccagattca gtaccgaagc ggcagcgcca acgctagcca
     1561 ccgtctgaag cgtttccagc atccctcgaa tctctagcag ctgcgcattg gcgccgagcg
     1621 agcttgcgac ttccagcggg gccaatggcc cggccatccc cacgtcaaag acccattgag
     1681 acagacgtga tgtgggctga aggtgcgcaa ggatttgatg cgacgcactg tctttcacca
     1741 aggccccgct cacgtacgcc gccccagtcc cgacgtgcgc caggaggctt gcaggaacag
     1801 ccatgagttg gatcacttgg aggcctccag gtcggcgagt tgcctccgca actcgtgggc
     1861 tgtgaggagt gcgagcgtcg ccggaatcgt cttgcgatat gacgtcgaca tcacggcatt
     1921 cgcagcgaac gcaacgagcg ccggcacaga tagaagtgct cccgcgactg ttgccaagcc
     1981 cccgcttgcc atcaactgaa cgccggacca agcgccgaac acccggacga cgatccagaa
     2041 gctgagttga tagagtgtgc cgccgatcaa accactcagg gcgaagctgg tcgccccctg
     2101 tgccaaggac agcgcgcctg cgccagagag taggcccgcg ccaatacctg caccgagcga
     2161 ggacgtcgta atgccgctga tgcccgcacc tctgacactg gcgcctacgt ttgcctgggc
     2221 ctgcgcgagt tcctgctcaa gactgcttgc aagtttggct cgttcttcct cgttcatctt
     2281 tgcccaggcc tcctgcgcgc gctcgtcgaa gtaccggagg atcgcggctt cgatctcccc
     2341 atcggagcgc tcgtcatcgg tccgaaagtc ggaccatccc cagccaggtt tgagcttgtc
     2401 agcaacgtca acgagtatcc gccgccacgg gagttcgtga ttccctctgc gcagatttag
     2461 caaggtgtgc ccgtggaccg ccctccactc cttgctgatg ttgaggatct tcagttcgcg
     2521 tgagccgatt gcgaaatcgt cccggtcggg tgcgcacttg agggcatcaa cccaaatcgt
     2581 ccgcaactcc tggtcgtcga gttcacgcaa cagggcaaac agccgatcgt catagggcat
     2641 ccgcgaatat tagccgccaa ggtcaccatt cttgcccctc gctgaacttg ttctcctatg
     2701 agcccccgcg tctcggggtc atgcttggcc ataagcgccg ctccgcggcg cttatggcca
     2761 aggtggcaat cgaggccttg gccgatggca agacgactgc caagttcgca cagaagcgcg
     2821 acgtgctcac aaccaggtag cggagtgacg ccgctggtga gcgagcccga gctggcactg
     2881 ataggccgga cgacgagttg cacccggagc cggatgctgt gcgacttcct atgcctgcat
     2941 ggcatcgagg tcgggggccg cgcgtgtgca cgcggacaac cacgaggaca ctgcggcctg
     3001 tcgtcgagcg cgtgacccac cttgagccgg cccgaagctc cagaagtctg aggcggctca
     3061 catggcgcac tttgcccgca caacttgagt gccactcgaa acgacacgac ctgtcgcgcc
     3121 ggccttcgac acttggtcac ccaatcaccg gagtctgcgc catgtttcta gccttcattc
     3181 ttctaatcgc cttggcgttg gtattcgtca agctgggcgc cctatccgtt tgggtcacgg
     3241 ttctctccgc aagcctccag acgctgctgg cgctcctagc cgccgtcgct gcggcggtcg
     3301 gatggaaggc ttggaggagg cgacgcgatg ggatatcgtt caccgaggcc cgcgttctcc
     3361 acaagcgaga ttgaagccgg ccccgtagca ccagcaggag gaggaccaat cgtgcccgcg
     3421 ccgtccattg ccgtttccgg cctgccgagc ttcggcgact ttgccgccag cgccatcgtc
     3481 tcggccatca aggccctgta ccgggccgcc ggtcagttca atgactggct ggacgagcac
     3541 atcgcggatc tcaagcgctc ggacgacccg ctgatcgcca gcatcggccg ggtcctagaa
     3601 ggtgcgaagt tcgggttcgg tatgggatat gtggcctcca cggcactgat agcggtagga
     3661 cagttcctgc tcggcaacac cttcgccgcc gttgcgaccg tggcctctgc ggcggcgttg
     3721 tcgaacccga tcgcgatgac ctgtgcggca atcggcgcca tctatttcgg gtggaaagcg
     3781 ctcaccgaca aggagcgcga acagattctc gaacacctgg cggccggctt gacgctcgga
     3841 gtggaactga tcagggccgt cgtggagttc gcgatccgca ggtcgcgcga actcctggat
     3901 tccagccagc tcgaggccgc caagacgttt gtcaaggcac aggccgaggc gttcgggcgc
     3961 actttgtacg acgtcacaaa acaattgggt gatctcgcct cgggcagcgc cgaggcaatc
     4021 gcagagaagg caggccaggc tgctgttgcg atcaagggcg cggcgggcaa ggtcggagat
     4081 gcagtcgcag gcggcgccag ctcagcgcag gaggcgatcg gtacaggagt ggcgcgtcta
     4141 cgcgagctgg cgaccgacgc cggaaagtcg ctcaagcgcg ccagcggacc gcccgtcctc
     4201 accaccgtac cgtgggagtc aggcgccgcg gtcgacaacg caccggtgct gccaggcgct
     4261 ccggaccatc agctcgggcc tgcgcacaag acgggcgcga ccggttcaga cccgcaacaa
     4321 tgaccagcgg caaccacgac acgctcgtcc acaggctgtc gctgatgctg ctcaagctca
     4381 accagggcga agccctggat cccagggcgc ttgccgaaga gttcggcgtc aacctgcgca
     4441 ccatccagcg cgacctcaac cgccgattca cgtacctgcc gctgcacaag tcagaggggc
     4501 gctaccggct cgatccggca ttcctgggac agttcacagc caaggacgtc caggcatttg
     4561 cggcgctggc gggggtgcag gaactctttc cgcacttgac cgacgaattc ctgcgcgacg
     4621 tgtt
//
LOCUS       DAOORV010000247         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_22284_length_4605_cov_6.959341, whole genome shotgun sequence.
ACCESSION   DAOORV010000247 DAOORV010000000
VERSION     DAOORV010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22284_length_4605_cov_6.959341"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKV56_18375"
     CDS             complement(<1..370)
                     /locus_tag="PKV56_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOX69818.1"
                     /translation="MRITGTYVTTTTLGEKVRAFMPHPLPPAKPVLGLESYDAAAHRA
                     EMALARLAGVAGLVPSVDWLLYSAIRKEALLTSQIEGTQATLTDLFDDEAGQVLTNTA
                     DVEEVTNYLRAFRLVRDNLRS"
     gene            566..1696
                     /locus_tag="PKV56_18380"
     CDS             566..1696
                     /locus_tag="PKV56_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOX69819.1"
                     /translation="MHKVVHSESVKSALAIKGWDQKQLAEELGVSGQAVTNWLRGVDF
                     PRPATLLKLASTLALGFDQLVLADQADQPVIAFRKKGAAKTTDEHVLRARAMGAMLKP
                     LVGYLPARQSLRTQISDTALAYESLQATAAAVRAKLGIGQEAVLSYEHLISQFAENDA
                     IIVPVMWGEKNRHENALHILLPADKVTFIYLNLDTHLEDFKFWMAHELAHVFTPALAG
                     KDEGEDFADAFAGALLFPKELAHHAYVNASHRRTPSGIISALHDFARAHMISLYSVYN
                     EVTTYVSAQGLSPLKVTSQQVHAARNSDSVRGELVSATLFRPLPPEPSTYISAAHGVF
                     KSVFFPALQVMLRERGTGAGYVQQVMSIALPDAQAIHDELLR"
     gene            1693..2286
                     /locus_tag="PKV56_18385"
     CDS             1693..2286
                     /locus_tag="PKV56_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69820.1"
                     /translation="MTLVLLDTNTYLRLAKRIRPLLGMKFGQKDYVLMVLKDVEDEVR
                     RNRTLRSKFPWFDDEEFGTERDSAQIRLTADEKSTLNNARSVLRGHVLADVDRYTSGG
                     RQPPSDTDCRVLAFGQIREAIVVTDDLGMHLLADDFDLPIWHGWELLSKMKAAKKVDN
                     DLIRDIYAALERNDDMTATWLKAKHTDFARVFGPKPE"
     gene            complement(2293..3630)
                     /locus_tag="PKV56_18390"
     CDS             complement(2293..3630)
                     /locus_tag="PKV56_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOX69821.1"
                     /translation="MSSDTPWLPLEDLVEDIIDRRGLTPLKLGGLFTSSGHRVISAKL
                     LKGGRIDLGADEPRYVNSDVYAKWMKGSLLPGDVLMTSEAPLGELAFINSELDWCLGQ
                     RLFAIRPKKDRLDGCFAYYALQTRQVREDLFGRSTGTTVQGIRQSELRRVRLPVPDLT
                     LQVAAASVLKALDDRIALLRETNATLEAIAQALFKSWFVDFDPVRAKQQGLAPAGMDE
                     ATAALFAGNIEAWNECVLRPIAADLINGRKLLIGDGYRAKNNELGLPGVSFVRAGDLD
                     AGQITPTKDFLAKPALNAAAGKMAKAGDTAFTSKGTIGRFAFVDEGSGDAVYSPQVCF
                     WRSLDRDIIEPAYLHYWMKSAAFMEQVKAVCGQAAIMEYVSLSDQRGMRLDLPPIAIQ
                     RRFAELADSLLRRISCNRATAMLLASLRDTLLPRLISGQLRLPDADRFKASPA"
     gene            complement(3620..>4605)
                     /locus_tag="PKV56_18395"
     CDS             complement(3620..>4605)
                     /locus_tag="PKV56_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX69822.1"
                     /translation="RSIVKTLVAVLAPHHGKVYDPCCGSGGMFVQSEEFIESHGGKIG
                     DVAIFGQEANPTTWRLAAMNLAIRGIDFNLGREPADTFTRDQFPDLRADFILANPPFN
                     ISDWWHASLSGDARWQYGDPPQGNANYAWLQHMLHHLAPRGRAGIVLANGSMSSSQNN
                     EGQIRAAMVDADVVEVMIALPGQLFFNTQIPACLWFLAKGKRSRPGEVLFIDARKLAT
                     MISRVQCELTDAVIERIAGTVAAWRGEAGAGEYADVAGYCRSVTLAEIAEHGHVLTPG
                     RYVGAEAVEDDDEGFEAKMQALTEKLGEQMAKGAELDAVIRQKLGGLGYEF"
BASE COUNT          874 a         1464 c         1404 g          863 t
ORIGIN      
        1 cgctgcgcag gttgtcgcgc acgagccgga aggcgcgcag gtagttggtg acctcctcga
       61 cgtcggcggt gttggtcagc acctgcccgg cctcgtcgtc gaacaggtcg gtcagcgtgg
      121 cctgggtgcc ttcgatctgc gaggtgagca gcgcctcctt gcgaatggcg ctgtagagca
      181 gccagtcaac cgaaggcacc agaccggcca cgccggccag ccgggccagc gccatctcgg
      241 cgcggtgcgc ggcggcgtca tatgactcaa ggcccagcac aggcttggcg ggcggcagcg
      301 ggtgcggcat gaacgcgcga accttttcgc caagtgtggt ggtggtgacg taggtaccgg
      361 tgatgcgcat ggtgtgaaag cgtctttgca agaccgccct aatattaaaa cattctttaa
      421 tttgttgtga aagtttgaaa gagcacttgt agaagccagc tgacttgacg caacttgttg
      481 cgatcgtcac gtttgatcag atcaaagatg ctgtcgcttg atttcacttg attacagcac
      541 tcgtcaccgc cacaatccaa tgtctatgca caaagttgtc cacagtgagt ctgtcaaatc
      601 ggcccttgcc atcaaaggct gggaccaaaa gcaactggct gaagaacttg gcgtgagcgg
      661 ccaggccgta accaactggc tgaggggcgt tgacttcccc cgccccgcca cgctgctgaa
      721 gctggcatcc acattggcac tgggcttcga ccagttggtg ctggccgatc aggcagatca
      781 accagtgatc gccttccgaa agaagggcgc cgccaagacc accgacgagc acgtcctgcg
      841 cgcacgcgcc atgggcgcga tgctcaagcc gctcgtgggc tacctgcccg cacgacagtc
      901 tctgcgcacg cagatctccg acaccgcact cgcgtacgaa agcctgcagg ccaccgctgc
      961 ggccgtccga gccaagcttg gcatcggcca ggaggccgtg ctttcgtacg aacatttgat
     1021 cagtcagttc gcagagaacg atgcgatcat cgttccggtg atgtggggcg agaagaaccg
     1081 gcacgagaac gcgctgcaca tcctgctgcc tgccgacaag gtcaccttca tctacctgaa
     1141 cctcgacacc caccttgagg acttcaagtt ctggatggcc cacgagctgg cgcacgtctt
     1201 caccccggcc ctggcgggca aggacgaagg tgaagacttt gccgatgcct ttgctggcgc
     1261 gctgctgttc cctaaggaac tggcccacca cgcctacgtg aacgccagcc accggcgcac
     1321 gccgagcggc atcatcagcg cgctgcacga cttcgcccgc gcgcacatga tctcgctcta
     1381 cagcgtgtac aacgaggtca ccacgtacgt ttccgcgcag ggcttgtctc cgctgaaggt
     1441 gacttcccag caagtacatg ccgctagaaa cagcgacagt gtgcgaggcg agttggtcag
     1501 cgccacactg ttccggcccc tgccgccgga gccctcgaca tacatttctg cggcgcacgg
     1561 tgtcttcaag tcagtcttct tcccggcctt gcaggtcatg ctgcgtgaac gcggaaccgg
     1621 cgccggctac gtgcaacagg tgatgagcat cgcgctgccg gatgcccagg ccatccacga
     1681 cgagttgctg cgttgacgct tgtcctgctc gacaccaaca cctatctccg gctggccaag
     1741 cgaatcagac cgctgctcgg aatgaagttc gggcagaagg attacgtact catggtgttg
     1801 aaggacgtgg aggatgaggt tcgccgtaac cggacgcttc gctccaagtt cccgtggttc
     1861 gacgacgaag aattcggaac cgagcgtgac tccgcccaga tcaggctgac cgccgacgag
     1921 aaatcgacgc taaacaacgc ccgcagcgtg cttcgcggtc atgtcctcgc cgacgttgac
     1981 cgatacacct ccggcgggcg ccagccaccg tccgataccg actgtcgcgt gctcgccttc
     2041 ggccagattc gcgaggccat cgtggtgacc gacgacctgg gcatgcacct gctggccgat
     2101 gatttcgatt tgcccatctg gcacggatgg gaactgctgt cgaagatgaa agcagccaag
     2161 aaggtcgaca acgacctcat ccgcgacatc tacgccgcgc tagagcgcaa cgacgacatg
     2221 actgcgacct ggctgaaggc taagcacacg gatttcgcca gggtcttcgg gccgaagccg
     2281 gagtaattgc gcttatgccg gcgacgcttt gaatcggtcg gcatcgggta atcgcagctg
     2341 gccggagatg aggcggggga gcagtgtgtc gcggagagat gccagaagca ttgcggtagc
     2401 gcggttgcac gaaattcgtc ggagcaacga atccgccaac tcggcgaagc ggcgttgaat
     2461 agcaatcggc ggaaggtcaa gccgcatccc gcgctgatcc gacaggctca cgtactccat
     2521 gattgcggct tgcccgcaga cagcttttac ctgctccatg aacgctgcac ttttcatcca
     2581 gtagtggagg taggcgggtt cgatgatgtc gcggtcaagg ctgcgccaga agcagacctg
     2641 aggcgagtag acagcatcgc ccgatccttc atccacgaac gcgaaccggc cgatagtgcc
     2701 ttttgacgtg aatgccgtgt cgccggcctt cgccatcttc cccgcagccg cgttcagcgc
     2761 gggcttcgcc agaaagtcct tggttggcgt gatctggcct gcgtccaggt cacccgcacg
     2821 aacgaacgag acgccaggca gtcccaattc gttgttcttg gcgcgatacc catcgccgat
     2881 caggagcttg cgtccgttga tcaagtcggc ggcaatcggc cgcaagacac attcattcca
     2941 tgcttcgata tttcccgcaa acaaggccgc cgtggcttcg tccatgcctg ccggggcgag
     3001 cccttgctgc ttggcgcgca cgggatcgaa atcgacgaac cacgacttga acagcgcctg
     3061 cgcgatggct tcgagggtgg cgttggtttc gcgcaggagg gcgatgcggt cgtcgagggc
     3121 tttgagaaca ctagcggcgg caacttgcag cgtcaaatct gggacaggca gccgcacgcg
     3181 acggagctcc gactgccgaa ttccttgcac ggtcgtacca gtcgatcgtc caaagagatc
     3241 ctcgcgaacc tgtcgcgttt gcagcgcgta gtaggcgaag cacccgtcca gtcggtcctt
     3301 cttcggtcga attgcgaata gacgttgacc gagacaccaa tcaagttccg agttgataaa
     3361 ggcgagttct ccaagcggcg cttcggaggt catcaaaacg tcgccgggaa gcaatgatcc
     3421 tttcatccac ttcgcgtaga catccgagtt cacgtaccga ggctcatccg ctcccaagtc
     3481 aatccgtccg cccttaagga gcttggcaga gatcacgcga tgcccggagc tggtgaagag
     3541 ccccccaagc tttaaaggcg tcagtcctct ccgatcgatg atgtcttcga caaggtcttc
     3601 tagcggcagc catggagtgt cagaactcat accccagccc cccgagcttc tgccggatca
     3661 ccgcatccag ctccgccccc ttcgccatct gctcgcccag cttctcggtc agtgcctgca
     3721 tcttggcctc gaagccctcg tcgtcgtcct ccaccgcctc ggcgcccacg tagcgacctg
     3781 gcgtcagcac atgcccgtgc tcggcgatct cggcgagcgt cacgctgcgg cagtagcccg
     3841 ccacgtcggc gtactcaccc gcgccggcct cgccgcgcca cgcggccacg gtgccggcga
     3901 tgcgttcgat caccgcgtcg gtcagctcgc actgcacgcg gctgatcatc gtggccagct
     3961 tgcgcgcgtc gatgaacagc acctcgccgg ggcgtgaccg cttgcccttc gccaggaacc
     4021 acaggcaggc cgggatctgc gtgttgaaga acaactggcc gggcagcgcg atcatcactt
     4081 cgaccacgtc ggcgtcgacc atcgcggcgc ggatctggcc ttcgttgttc tggctgctgc
     4141 tcatgctccc gttggccagc acgatgccgg cgcggccgcg cggcgccagg tgatgcagca
     4201 tgtgctgcag ccacgcatag ttggcgttgc cctgcggcgg gtcgccgtac tgccagcgcg
     4261 catcgccact caggctggcg tgccaccagt cgctgatgtt gaagggcggg ttggccagga
     4321 tgaagtcggc gcgcaggtcg gggaactggt cgcgggtgaa ggtgtcggcg ggctcgcggc
     4381 ccaggttgaa gtcaatgccg cggatagcga gattcatcgc ggccaggcgc caggtggtcg
     4441 gattggcctc ctggccgaag atggccacgt cgccgatctt gccgccgtgc gattcgatga
     4501 attcttcgct ctgcacgaac atgccgcccg agccgcagca cgggtcgtag accttgccgt
     4561 ggtgcggcgc cagcacggcc accagcgtct tgacgatgct gcgcg
//
LOCUS       DAOORV010000248         4586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_22420_length_4586_cov_7.011697, whole genome shotgun sequence.
ACCESSION   DAOORV010000248 DAOORV010000000
VERSION     DAOORV010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22420_length_4586_cov_7.011697"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKV56_18400"
     CDS             complement(<1..619)
                     /locus_tag="PKV56_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69823.1"
                     /translation="MKIRLIAIAVSALLAQGASAAVVYQSLSEFEALAGTPGTTIDFG
                     TPILPSCADVGYFKAQGDVSGDCVATSTLINGQRVYVMRGWAWAPTIHVRSGSAEGYG
                     PNGPGSGYDAHTWAGPGAYLAPDPMGGTHLAIEQNRPFMTLGVFTTVGFFGWLAEPQQ
                     ELLTEGLLILPEGAQITKLEFDYQRQVPEPGTLSLAGLAALLLASA"
     gene            complement(659..3841)
                     /locus_tag="PKV56_18405"
     CDS             complement(659..3841)
                     /locus_tag="PKV56_18405"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOX69824.1"
                     /translation="MTEDQLEQECLGWLADVGYAHLYGPDIAPDGTAPERGDYRQVLL
                     IERLRQAVAKLNPGIPQAARDDAVKQVIDLGTPVLLAANRHFHRLLVAGVPVQYQRDG
                     ETRGDFVRLVDWSEPARNEWLAVNQFSITGPHHTRRPDIILFVNGLPLVLIELKNPAD
                     LNADVWKAFHQIQTYKAQIADTFQYNEVLVISDGTDALLGSLSADSERFMAWRTIDGV
                     TLDPLGPFNELQTLVRGVLAPAYLLDYLRFFVLFEDDGGLVKKIAGYHQFHAVRAAIA
                     QVIAASRPDGLPSVRGKGGVVWHTQGSGKSITMTCFAARVMQEPLMANPTIVVITDRN
                     DLDGQLFGVFSLAQDLLREQPVQANTRQELRALLSNRPSGGIVFATIQKFMPGEDEDS
                     FPMLSDRSNIVVIADEAHRTQYGFEAKLKTVKQRPASGNPATVLTTGSGEVPSTQAEF
                     APTVDRYQVGYAQHLRDALPHATFVAFTGTPVSGEDRDTRAVFGDYISVYDMQQAKED
                     GATVAIYYESRLAKLGLKADELATIDDEVDELAEDEEESQQAKLKSRWAALEKVVGAE
                     PRIASVAADLVAHFEERNTAQTGKAMVVGMSRDICVHLYNEIVKLRPDWHSADPERGA
                     IKIVMTGSASDKALLRPHIYSSQVKKRLEKRFKDPSDPLRIVIVRDMWLTGFDAPCVH
                     TLYIDKPMKGHNLMQAIARVNRVFKDKQGGLVVDYIGIANELKSALKEYTASQGRGRP
                     TVDAHEAFSVLAEKLDALRGMLHGFDYSDFLTGGHKTLAGAANFVLGIQDGKKRFADT
                     ALAMSKAFTLCCTLDEAKAVREEAAFFQAVKVILTKRDITAQKKTDEARELAIRQIIS
                     SAVVSEEVVDIFDAVGLEKPNIGILDDAFLAEVRNLPERNLAVELLERLLEGEIKSRF
                     ATNVVQNKKFSDMLSDVVQRYQNRSIEAAQVMEELVQMAKDFREAAARGEQLGLTEDE
                     VRFYDALANNESAVRELTDETLKKIAHELAENLRKNLTVDWSQRDSVQAKLRLMVKRI
                     LRKYKYPPDQQDAAVELVLQQAKALGEAWA"
     gene            complement(3838..>4586)
                     /locus_tag="PKV56_18410"
     CDS             complement(3838..>4586)
                     /locus_tag="PKV56_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOX69825.1"
                     /translation="DAHRLLLDGARGAGKQPGELRRSQNWIGGARPGRAVFVPPPPDQ
                     VPGLLADLERFIHDPAPALPPLVRVALVHLQFETIHPFLDGNGRIGRLLIAALLEQWR
                     LLPEPLVYLSSYLKQNQVEYYRRLSAVRTDGDWEGWVAFFLDGVETAATDAERGIVAV
                     ASLVATDRRRLLAAPRVGAVGLRLFELLPLMPRFTIEQVRQKLATTFPTATAAVKLLE
                     ELGIVTELTGQKKNRCFSYAAYVELLAGET"
BASE COUNT          740 a         1543 c         1438 g          865 t
ORIGIN      
        1 gtgcgctggc caagagaagt gcggccaagc ccgccaacga aagcgtgccc ggctccggca
       61 cttgccgctg atagtcgaac tcaagcttcg tgatctgcgc tccctcggga agaatcagca
      121 agccttcggt cagcaactcc tgttgaggtt cggccagcca cccgaaaaag ccgaccgtgg
      181 tgaacacgcc cagcgtcata aagggacggt tctgctcaat cgcaaggtga gtaccgccca
      241 ttgggtctgg cgcgaggtac gcgccagggc cagcccatgt gtgcgcgtca tagccagagc
      301 ctggtccgtt cggtccataa ccctctgcgc tgccagagcg gacgtggatg gtcggagccc
      361 aagcccagcc acgcatcaca tacacgcgct ggccgttgat cagagtcgaa gttgccacac
      421 agtcaccgct gacgtcaccc tgtgctttga agtagccaac atccgcgcat gacggaagaa
      481 tcggcgtgcc gaaatcgatc gttgtgccag gggttccagc aagcgcctcg aattcgctga
      541 gtgactggta gacaacggcg gcgctcgcgc cttgggccaa cagcgccgaa acggcgatgg
      601 caatcaatct gatcttcatg tgttttcccg gctcatcaag ttcatcgtgg tttcttacct
      661 acgcccaagc ttcacccaac gccttcgcct gctgcaacac caactccacc gcggcgtcct
      721 gctgatccgg cgggtacttg tacttgcgca ggatgcgctt gaccatcaac cgcagcttgg
      781 cttgcacgct gtcgcgctgt gaccaatcca ccgtcaggtt cttgcgaagg ttctcggcca
      841 gctcgtgcgc gatcttcttg agggtctcgt cagtcagttc acgcaccgcc gattcgttgt
      901 tggccagcgc gtcgtagaag cgcacctcgt cttcggtcag cccaagctgc tcgccgcgcg
      961 ccgccgcctc gcggaagtcc ttggccatct gcaccagttc ttccatcacc tgggcggcct
     1021 cgatggatcg gttctggtat cgctgcacca cgtcgctcag catgtccgag aacttcttgt
     1081 tctggaccac gttggtcgcg aaccgtgact tgatctcgcc ttccagcaac cgttccagca
     1141 gttccaccgc caggttgcgc tcgggcaggt tgcgcacctc ggccaagaag gcgtcgtcga
     1201 ggatgccgat gttcggcttt tccagcccca ctgcgtcgaa gatgtcgacc acctcttcgg
     1261 acaccaccgc cgagctgatg atctgccgga tcgccagctc gcgcgcctcg tcggtcttct
     1321 tctgcgccgt gatgtcgcgc ttggtcagga tcaccttgac ggcctggaag aacgccgctt
     1381 cctcccgcac cgccttcgcc tcgtcgagcg tgcagcacag cgtgaacgcc ttgctcatcg
     1441 ccagcgccgt gtcggcgaat cgcttcttgc catcctggat gcccagcacg aagttggccg
     1501 cgccggccag cgtcttgtgg ccgccggtca ggaagtcgct gtaatcgaag ccgtgcagca
     1561 tgccccgcag cgcgtcgagc ttttcggcca gcacgctgaa cgcctcgtgc gcgtccacgg
     1621 tcggccggcc gcggccctgg ctggcggtgt actccttcaa cgccgacttc aactcattgg
     1681 cgataccgat gtagtcgacc accagcccgc cctgcttgtc cttgaacacc cggttcactc
     1741 gggcgatggc ctgcatcagg ttgtggccct tcatcggctt gtcgatgtaa agggtgtgca
     1801 cgcacggcgc gtcgaagccg gtcagccaca tgtcgcgcac gatcacgatg cgcagtgggt
     1861 cgctcgggtc cttgaaccgc ttttccagtc gcttcttgac ctggctgctg taaatgtgcg
     1921 ggcgcagcag cgccttgtcg ctggccgagc cggtcatcac gatcttgatg gcgccacgtt
     1981 cggggtcggc actgtgccag tcggggcgca gcttgacgat ctcgttgtac aggtggacgc
     2041 agatgtcgcg gctcatcccg accaccatcg ctttgccggt ctgagcggtg ttgcgttcct
     2101 cgaagtgcgc caccaggtcg gctgccacgc tggcgatgcg cggctcggcg ccgaccacct
     2161 tttccagggc agcccagcgg ctcttgagct tggcctgctg gctttcctct tcgtcctcgg
     2221 ccagttcgtc gacctcgtcg tcgatggtcg ccagctcgtc ggccttcaag cccagcttgg
     2281 ccagccggct ctcgtagtag atggcgaccg tcgcaccgtc ttccttggcc tgctgcatgt
     2341 cgtacacgct gatgtagtcg ccgaacaccg cacgggtgtc gcggtcctcg cccgacaccg
     2401 gcgtgccggt gaaggcgacg aaggtggcgt gcggcagtgc atcgcgcagg tgctgggcgt
     2461 agccgacctg atagcggtcg accgtgggcg cgaactcggc ctgggtcgac ggcacctcgc
     2521 cgcttccggt cgtgagcact gtcgccggat tgcccgatgc cggccgctgc ttcacggtct
     2581 tgagcttggc ctcaaagccg tactgcgtgc ggtgcgcttc gtcggcgatc accacgatgt
     2641 tgcttcggtc cgagagcatc gggaagctgt cttcgtcctc gcccggcatg aacttctgga
     2701 tcgtcgcgaa cacgatgccg cccgacggcc ggttgctcag cagcgcgcgc agctcctgcc
     2761 gcgtgttggc ctgcaccggc tgctcgcgca acaggtcctg agccagcgag aacaccccga
     2821 acaactgacc gtcgaggtcg ttacggtcgg tgatgacgac gatggtcggg ttggccatca
     2881 atggttcctg catcacgcgc gccgcgaagc aggtcatcgt gatgctcttg ccgctgccct
     2941 gcgtgtgcca caccacgccg cccttgccgc gcacgctggg caggccatcg gggcgggatg
     3001 ctgcgatcac ctgtgcgatg gccgctcgca ccgcatggaa ctggtggtag ccggcgatct
     3061 ttttgaccag accgccgtca tcctcgaaca gcacgaagaa ccgcaggtag tcgagcaggt
     3121 aggcaggcgc cagcacgccg cgcaccagcg tctgcaattc gttgaacggg cccagggggt
     3181 cgagggtgac gccgtcgatc gtgcgccagg ccatgaagcg ttcgctgtcg gccgacagcg
     3241 agcccagcag cgcgtcggtg ccgtccgaga tcaccagcac ctcgttgtac tggaaggtgt
     3301 cggcgatctg cgccttgtag gtctggatct ggtggaacgc cttccatacg tcggcattca
     3361 ggtcggccgg gttcttcaac tcgatcagca ccaaaggcag gccattgacg aacaggatga
     3421 tgtcgggccg gcgcgtgtgg tgcggcccgg tgatcgagaa ctggttgacc gccagccatt
     3481 cgtttcgtgc cggctcggac cagtcgacca gccggacgaa gtcgccgcgg gtttcgccat
     3541 cacgctggta ctgcaccggc acgccggcca ccagcagccg gtggaagtgg cggttggcgg
     3601 ccagcagcac gggcgtgccc aggtcgatca cctgcttgac ggcatcgtct cgcgcggcct
     3661 gcggaatgcc ggggttgagc ttggcaaccg cctgccgcag ccgctcgatc agcagcacct
     3721 gccggtaatc gccccgctcg ggcgcggtgc cgtccggtgc aatgtcgggc ccgtacaggt
     3781 gggcatagcc cacatcggcc agccaaccca agcattcctg ttcgagctgg tcttcggtca
     3841 tgtttcccct gccagcagtt cgacgtaggc agcgtagctg aaacagcggt tcttcttctg
     3901 cccggtcagc tcggtgacga tgccgagttc ttccagcagc ttcactgcag cggtggccgt
     3961 ggggaaggtg gtggccagct tctggcgcac ttgctcgata gtgaagcgcg gcatcagcgg
     4021 cagcaactcg aacaggcgca gacccacagc gccgacacgc ggtgccgcca gcagccgtcg
     4081 gcgatcggtt gccaccaaac tggcgaccgc gacgatgcca cgctcggcat cggtggcggc
     4141 cgtctccacg ccgtccagga agaaggcgac ccagccctcc cagtcgccat cagtgcgcac
     4201 cgccgacagg cgccggtagt actccacctg gttctgcttg agatagctgc tcaggtacac
     4261 cagcggctcc ggcagaaggc gccactgttc cagcagcgcc gcgatcagca ggcggccgat
     4321 gcgcccattg ccgtccagga aggggtggat ggtctcgaac tgcaaatgca ccagggccac
     4381 acgcacgagc ggcgggagcg ctggcgccgg atcatggatg aagcgctcca ggtcggccag
     4441 cagccccggc acctgatccg gcggcggcgg cacgaacaca gcccgccccg gacgcgcgcc
     4501 accgatccag ttctgtgagc gccgcagctc gcccggctgc ttgccggcgc cgcgtgcgcc
     4561 atcgagcagc agccggtggg cgtcgc
//
LOCUS       DAOORV010000249         4476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_23111_length_4476_cov_4.876272, whole genome shotgun sequence.
ACCESSION   DAOORV010000249 DAOORV010000000
VERSION     DAOORV010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23111_length_4476_cov_4.876272"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PKV56_18415"
     CDS             <1..481
                     /locus_tag="PKV56_18415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOX69826.1"
                     /translation="DNAGMGTFYRSQHELVYLFKHGDAPHINNFELGQHGRYRTNVWN
                     YPGVNTFKGKGYKLLALHPTVKPVGLIADALRDCSHRKGVVLDPFAGSGTILIAAERT
                     GRRARAIELDAQYVDVAIHRWQRLTGKQAVLAATGQTWEQVRTERVSSQTQETHHEL"
     gene            471..875
                     /locus_tag="PKV56_18420"
     CDS             471..875
                     /locus_tag="PKV56_18420"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HOX69827.1"
                     /translation="MSYEIGYRRPPASGRFKKGASGNPKGRPKGSSNFLTLLDQELAQ
                     TIIVNENGKRKTVSRLQAMVKRMVADALQGDQKSLLTLVEILRRTGGLETPDVDGLLP
                     DNYESILNSYVETRLKAGARKPTLSPRKEGES"
     gene            872..2305
                     /gene="terL"
                     /locus_tag="PKV56_18425"
     CDS             872..2305
                     /gene="terL"
                     /locus_tag="PKV56_18425"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HOX69828.1"
                     /translation="MSELSKQAYLAAVKADALVFLQQAFTTIYPGKEFMPNWHLDALL
                     HGLEEGLRGKMPRLIINLPPRHLKSFIVSVVWPAFILGQDPSAKIICISYSDDLAKAL
                     SRDFKRIVESDWYRAIFPNVRLTKVTEGEVITDAGGGRYATSVGGTLTGRGADFILID
                     DPIKPEEATSDKARQAVNEWYRSTLLSRLDDKQRSVLILVMQRLHVNDLTGYVEAGGG
                     FQKLSFPAIATRDDTVELRNGGRYFRREGEPLHGDREGIEVLKRIRDEVGNFNFSSQY
                     QQSPATPDGSMFKRKWFKLTDQAPEINSTGTLYVSIDSAISTSDTADYSAFSVIYAHA
                     TKYFVVQAERGRWDYESLKAKALGYVKQFSRSGFPVHFVIERAGSGISLLKYLEDLRH
                     ERIRCFNYSPKSDKVIRAAYAIPIVESGRVYIVNVPGQNAWVEPYINEFVSFPHGRFD
                     DQVDSLTQFLPWADKRHNPRGGFYVIN"
     gene            2585..2968
                     /locus_tag="PKV56_18430"
     CDS             2585..2968
                     /locus_tag="PKV56_18430"
                     /inference="COORDINATES: protein motif:HMM:NF023420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HOX69829.1"
                     /translation="MKANATGSKATTTKVRSTKKIAVPVIPVVIAKPGRRGRAKVDVP
                     LPIVPPAPVEPVGTSKQARLISLLNAAPGATLEQMMLLTGWQAHTVRGTISGVLRKRL
                     GLNVVCDAADAADARRYRIVAAVGA"
     gene            2965..3369
                     /locus_tag="PKV56_18435"
     CDS             2965..3369
                     /locus_tag="PKV56_18435"
                     /inference="COORDINATES: protein motif:HMM:NF022598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HOX69830.1"
                     /translation="MNVSDALAELAALDRPQLVERWAAVFDHPAPRHAQVAMLRAALA
                     WRLQMQSLAGAGRVDRMVRGLRRSTACAAPSVSLAPGTRLLREWQGQTHHVTVLASGF
                     EYSGRTWRSLTAISRSITGTAWSGPLFFGLRS"
     gene            3366..>4476
                     /locus_tag="PKV56_18440"
     CDS             3366..>4476
                     /locus_tag="PKV56_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOX69831.1"
                     /translation="MTKIAVLPCAIYTRKSSEDGLEQGFNSLDAQREACEAFVLSQKT
                     QGWKVLGAYEDGGFSGGNVERPGLRRLLADVALGRVRIILVYKIDRLTRSLADFAKMV
                     ELFDAHDVSFVSVTQQFNTTSSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKRKGL
                     WMGGLAPVGYLPNDRTLAIDEPRAELVRDLYRLYLELGCVSALVAEVARRGWVTPARI
                     TRREGAAGGRPFSRGHLYRILSNPVYIGQIAHKDQVFAGQHPAIVERDLWDAVQAQMK
                     ANQQGHRKRVNSSQPSLLTGLVFDEQGHRLTPSHAQKGARRYRYYIDQVAAEDEGAAA
                     ALRAPAKELETAVIEAVSGFLKDRSRVMEVMGSVDA"
BASE COUNT          883 a         1255 c         1503 g          835 t
ORIGIN      
        1 ggacaacgcc ggcatgggca cgttctaccg cagccagcac gagctggtgt acctgttcaa
       61 gcacggggat gcaccgcaca tcaacaactt cgagttgggg cagcacggcc gctaccgaac
      121 caatgtctgg aactacccgg gcgtcaacac gttcaagggc aagggctaca agctgctcgc
      181 gctgcacccc accgtcaaac cggtgggtct gatcgccgat gccctcaggg attgcagcca
      241 tcgcaagggc gtcgtcctgg acccctttgc cggcagcgga accatcctga tcgctgccga
      301 gcgcaccggg cgccgtgcac gcgcgatcga gctcgatgcg cagtacgtgg acgtcgccat
      361 ccaccgctgg cagcgcctga ccggcaagca ggctgtgctg gcggcaaccg gacagacctg
      421 ggagcaggta cgcactgaac gagtgtcttc acagacccag gagacacacc atgagctatg
      481 agatcggcta ccggcgtccc ccggccagcg ggcgcttcaa aaagggggcc tcaggcaatc
      541 cgaagggtcg gcccaagggt tcgagcaact tcctgactct gctcgatcag gagcttgcgc
      601 agaccatcat cgtcaacgaa aacggcaagc gaaaaacggt cagccgactc caggcgatgg
      661 tgaagcgcat ggtggcagac gcccttcagg gtgaccagaa atcgcttctg accttggtcg
      721 agatattgcg ccgcacaggg ggcttggaga cgccagacgt cgacggcctg cttccggaca
      781 actacgaatc gatcctgaat agctatgtcg aaacaaggct gaaggctgga gcccgcaagc
      841 cgacgctgtc gccccgaaag gaaggtgaat catgagcgaa ctcagcaaac aggcctacct
      901 tgctgcagtc aaggcggatg ccttggtttt tctccagcag gccttcacca ccatttatcc
      961 gggcaaggaa ttcatgccca actggcatct cgatgccctg ctccatggcc tggaggaagg
     1021 tttgagagga aagatgccac gcttgatcat caatcttcct ccgcgacacc tgaagtcctt
     1081 catcgtatcg gtcgtctggc ccgctttcat tctgggtcag gacccctcgg caaagatcat
     1141 ctgcatcagc tactccgacg acctggcgaa agcgctttcg cgcgacttca agcggatcgt
     1201 agaaagcgat tggtaccgag cgatcttccc gaacgtgcgc ttgacgaagg tgacagaggg
     1261 ggaagtcatc accgacgctg ggggtgggcg ctacgcgact tcggtgggag gcactttgac
     1321 cggtcgcgga gcagacttca tcctcatcga tgacccgatc aagccggaag aggccacgtc
     1381 tgacaaggca cggcaggccg tgaatgaatg gtatcgaagc acgctcctga gccggctcga
     1441 cgacaagcag cgcagtgtgc tgatcctggt catgcagcgt ttgcatgtga acgacttgac
     1501 gggctacgtt gaagccggag gcggttttca gaagctgtcg tttccggcca ttgccacccg
     1561 agatgacact gtcgagctca ggaacggggg gcgatatttc cgcagagaag gcgagccact
     1621 gcacggcgat cgcgaaggca tcgaagtact gaagcgaatt cgcgatgagg tcgggaactt
     1681 caatttttct tctcagtacc agcaatcgcc ggcaacaccg gatgggagca tgttcaagcg
     1741 caagtggttc aagctcacag accaggcgcc tgagatcaac tccactggaa cgctttacgt
     1801 cagcatcgac agtgcgattt ccacctccga tacagccgac tattcagcgt tcagtgtgat
     1861 ctacgcgcac gcgacaaagt atttcgttgt gcaggccgag cgcgggcgct gggactacga
     1921 gtcattgaag gccaaggcgc tgggctatgt gaagcagttc agccgcagcg gctttccagt
     1981 tcatttcgtc atcgagcgtg ctggcagtgg catctcgttg ctcaaatacc ttgaggatct
     2041 gaggcacgaa cgtattcgat gcttcaacta ttcgcccaaa tcggacaagg tgattcgagc
     2101 agcctatgcg ataccgatcg tggagtcggg acgggtatat atcgtcaatg tgcctggaca
     2161 aaacgcctgg gttgagccgt acatcaacga gtttgtgagc tttccgcatg gtcgatttga
     2221 tgatcaggtc gatagcctga cgcagttctt gccatgggca gacaagcgac acaaccctcg
     2281 aggtgggttt tacgtgatca attgatctgt cggtggagat gggagaaaga ccaccagggt
     2341 gaagtgattg gatccagctc agttgttcac tgcatcggta aaccagcatc ggagccaatc
     2401 gaccgtcacg gatgcattcc tgtttgataa gtaatcaatg caaaaggcac ggcaccaggt
     2461 cgagagcgaa ccaccactct gcgtggcgca ggctgaagcc agacaaatga cttgatgcgt
     2521 gtcgcacgca gagcgttact ggtgcaccgc cgcgaaggtc gcggtggaca ccaggagaag
     2581 ctcgatgaag gcgaatgcaa caggctcgaa ggccacgacg acgaaggtcc ggtccacgaa
     2641 gaagatcgca gtgccggtga ttccggtggt gatcgcgaag cccggacggc gcgggcgcgc
     2701 gaaggtcgac gtgcccctgc cgatcgtccc gcccgcaccg gttgaaccgg tcggcacgag
     2761 caagcaggct cggctgatca gcttgttgaa cgcagccccg ggcgcgacgc tcgagcagat
     2821 gatgttgctg accggctggc aggcccacac ggtgcgcggc acgatcagcg gggtgcttcg
     2881 caagcggctc gggttgaacg tggtgtgcga tgcggcggat gctgctgatg ctcgccgcta
     2941 ccgcatcgtc gctgcggtcg gggcatgaac gtatcggacg cgctcgccga gctcgctgct
     3001 ctggatcggc cccagctggt cgagcgctgg gcagcggtgt tcgaccaccc tgccccgcgt
     3061 catgcccagg tggcgatgct gcgcgctgct ctggcgtggc ggcttcagat gcagtctctg
     3121 gccggggccg gtcgggttga tcggatggtg cggggtctgc gccgttcgac tgcttgcgct
     3181 gctccttctg tgtcgctggc tccgggcacg cggctgctgc gcgagtggca ggggcagacg
     3241 catcacgtca cggtgctggc ctcgggcttc gaatactccg gccggacctg gcgcagcctc
     3301 accgcaatct cgcgttcgat cacgggcacg gcgtggtccg ggccgctgtt cttcgggttg
     3361 cgctcgtgac gaagatcgcc gtgctgccct gcgcgatcta cacgcgcaag tcgtcggagg
     3421 acgggctgga gcaaggcttc aattcgctgg atgcgcagcg tgaggcctgc gaggcctttg
     3481 tgttgagtca gaagacgcaa ggctggaagg tgctcggtgc gtacgaggac ggcgggttct
     3541 cgggtgggaa cgtggagcgg ccggggttgc gtcggctgct ggccgacgtg gcgctgggtc
     3601 gggtgcggat catcctggtc tacaagatcg atcgactgac gcgttcgctg gcggacttcg
     3661 cgaagatggt cgaactgttc gatgcgcacg acgtctcgtt cgtgtcggtg acgcagcagt
     3721 tcaacacgac gtcgtcgatg ggtcggttga cgctgaacgt gctgctgtcg ttcgcgcagt
     3781 tcgagcgcga ggtcaccggg gagcgcatcc gcgacaagat cgcggcgtcc aagcgcaagg
     3841 ggctgtggat ggggggactt gcaccggtcg ggtatctgcc gaacgatcgg actctggcga
     3901 tcgacgagcc tcgggcggaa ctggtacggg atctttaccg gctgtacctg gagctgggtt
     3961 gtgtgagcgc gctggtggcg gaggtggccc gaagggggtg ggtgacaccg gcgcggatca
     4021 cgcggcggga aggtgccgcg ggaggccgtc cgttcagccg ggggcatctg taccggatcc
     4081 tgtcgaaccc ggtctacatc gggcagatcg cgcacaagga tcaggtgttc gcggggcaac
     4141 atccggcgat cgtggagagg gatctctggg atgcggtgca ggcgcagatg aaggcgaatc
     4201 agcaggggca tcgcaagcgg gtgaattcat cgcagcctag cttgttgacg gggctggtgt
     4261 tcgatgagca gggacatcgg ctgacgccgt cgcatgcgca gaagggagct cgtcgctatc
     4321 ggtattacat cgatcaggtg gccgcggaag atgaaggtgc ggcggccgcg ttgcgggcgc
     4381 cggcgaagga actggagacc gctgtgatcg aggcggtctc ggggttcctg aaggatcggt
     4441 cgcgggtgat ggaggtgatg gggtcggtcg atgcgg
//
LOCUS       DAOORV010000250         4385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_23724_length_4385_cov_6.848499, whole genome shotgun sequence.
ACCESSION   DAOORV010000250 DAOORV010000000
VERSION     DAOORV010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23724_length_4385_cov_6.848499"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKV56_18445"
     CDS             complement(<1..559)
                     /locus_tag="PKV56_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOX69832.1"
                     /translation="MLEDIKKTLWATADKLRANMDAAEYKHLVLGLIFVKYISDTFQG
                     KHAELMLRLADPEDEYFYGEADPADIAAELEDRDYYREANVFWVPEPARWEALRAAAK
                     QPDIGKRIDDALSLIEAENPKLKGILDKRYARAQLPDGKLGELVDLVSTIGFGASAAE
                     ARDVLGQVYEYFLGMFASAEGKRGGQ"
     gene            988..1491
                     /locus_tag="PKV56_18450"
     CDS             988..1491
                     /locus_tag="PKV56_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69833.1"
                     /translation="MLLAINPGGGGDAYASRTPQDCELIPLLEAFVRANPAELPEAFE
                     RMCASYSAHAQTWNLWRILRPTLEACGTPVEEVCYLNLFPYRTAKDARPASRALTAAW
                     TQVVAPLLQALQPSRLVALGKKSGGVAERFHREPPPLFVVPRTIGDTYVSEDAQRVLA
                     AIRERAT"
     gene            complement(1830..3074)
                     /locus_tag="PKV56_18455"
     CDS             complement(1830..3074)
                     /locus_tag="PKV56_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX69834.1"
                     /translation="MTAQAWRTFICRACGWIYDEALGDADGGLPPGTRFEDIPDDWAC
                     PLCGVSKADFEPCEPLPVLRRTSASAAASAGHARGGRRDAGVVIVGAGRAGWQMAQSL
                     REHDAHLPITIVTACPGNVYDKPLLSVALAKGIAPASLVRESAEQAAARLGVRLLART
                     QAISVSVGARALRTTRGTLRYRHLVLAHGADPIDLPNLPPALCWRINHLDAYLNFRDR
                     LGEPGALPPQRVLIAGAGLVGCELANDLALAGYPVTLLDLCERPLSSLLTEAQSNELL
                     AAWRDLPLTFVGGVDITGVRASGSSRTVSAQDGRQFEAEHVIAAVGLRTPSRLAQSAG
                     LSWNNGIAVDPLTLHTDVATIHALGDCISIGGEALRFIEPIARQARQIAATIRGETSE
                     PYVRAAPPVRVKTGSKGFTVVR"
     gene            complement(3115..4218)
                     /locus_tag="PKV56_18460"
     CDS             complement(3115..4218)
                     /locus_tag="PKV56_18460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOX69835.1"
                     /translation="MLEKVRAIAVGDLAAMADAIDRQGTYPKAVLKQLGAAGALSAHL
                     RLDGQHDYVAAIQAMAEVSRVCGATGFMVWCHDACGLYMEESGNPALMGQALRDHASG
                     HTLGATGLSNPMKTFAGIETFLLHARKVEGGYRVNGTLPWVSNLGPDHYFGAVATVEN
                     TDSATSEASGHEIMFIVRCDAEGVELRNCPSFSAMEGTNTWAVRLTDHFIGADTLVAD
                     PARPYIGRVRAAFILLQCGMGLGVTQGAIDSMWDVERALGHVNEFLDERPDELQAELD
                     ALTERTMRLARTPFATDTEYLIDVCDTRAHAAELALRAAQSALLHHGARGYLMSSAVQ
                     RRVRESHFVAIVTPAIKHLRKEIARLSATVAQA"
BASE COUNT          724 a         1436 c         1445 g          780 t
ORIGIN      
        1 actgcccgcc gcgcttgccc tcggcgctgg cgaacatgcc caggaagtat tcgtagacct
       61 ggcccagcac gtcgcgcgcc tcggcggcgc tggcgccgaa gccgatggtc gacaccagat
      121 cgaccagttc gcccagcttg ccgtcgggca gttgcgcgcg ggcataacgc ttgtcgagga
      181 tgcccttgag cttcgggttc tcggcctcga tcagcgagag tgcgtcgtcg atgcgcttgc
      241 cgatgtcggg ctgcttggcg gccgcgcgca gggcctccca gcgggcgggc tcgggcaccc
      301 agaagacgtt ggcctcgcgg tagtagtcgc ggtcttcgag ttcggccgcg atgtcggcgg
      361 ggtcggcctc gccgtagaag tattcgtctt ccggatcggc cagccgcagc atcagctcgg
      421 cgtgcttgcc ctggaaggtg tcggagatgt acttgacgaa gatgaggccg agcaccaggt
      481 gcttgtactc ggcagcgtcc atgttggcgc gcagcttgtc ggcagtggcc cagagtgttt
      541 tcttgatgtc ttcgagcatg aggctgccgg ttggcttgag gggctgcagg attatgggta
      601 acgaagcggc ggagcaccct cgtcgtttgc ccgccgaggc catgatgcac ggtcagtagc
      661 tcgtggggtg agcctgcgga cagtgtgcgt tgaagcgttg aggcgttccg gcatcatcgt
      721 tcgatgggct cgactgggac acgtgctggt agtcaatcca gcccccggca ggacctcagt
      781 cgaataacag gtacatctca ttcatcaggg gtgcacatgc cgcacagctc cgcctcgctt
      841 gccaccgcgt tccaccgtgc agcccaggtc acgcgggcgg cgctctatgg aacccagaca
      901 gactcgcaac ttcccctgca cctgcagtcc gacgcgagag tccccttctt gggcttctgt
      961 ggaccgcgct acagacgagg cggccctgtg cttctcgcca tcaacccggg tggcggcggt
     1021 gacgcctatg cgtcccgcac gccccaggac tgcgagttga tcccgctgct tgaggcgttc
     1081 gtccgggcca atcctgcaga gttgcccgaa gccttcgagc gcatgtgtgc aagctactcg
     1141 gcccatgcgc aaacatggaa cctgtggcgg atcttgcggc cgacgctgga agcctgcgga
     1201 acgccggtcg aggaagtttg ctacctgaac ttgttcccct accgcacggc caaagacgca
     1261 cgcccagcgt cgcgcgctct caccgcggcc tggactcaag ttgtggcacc cctgcttcag
     1321 gctcttcaac cttcgcggct cgtcgcgctt ggcaagaagt ctggtggagt ggcggaacgc
     1381 ttccaccggg aaccccctcc actgttcgta gtcccgcgaa ccatcggcga cacctatgtt
     1441 tcagaggatg cgcagcgcgt gcttgctgcc attcgcgaac gcgccacgtg atcaagtgtt
     1501 tgagcgcggc gttgcctcgg tcggggttct gtagactgac ctcacggcgg caggatccag
     1561 tcgatctcgc ttcctcccag gatctaagca gcgaacttcc cgaccggata actttccgga
     1621 taacttgcat acacaaaaat catgtagcga gcatccatgc ggttttccgg gcagagtgtg
     1681 atagtccccg gtccaccaaa atgagaaatc ccaacccttg tccggttggg atttttttgc
     1741 ctgttctgct tcaagcccac cggcagcctc aggctcgacg acctcaagaa gacgctcagg
     1801 gccgctgcgt cgccttccgg ataccggtct cagcggacga ccgtgaagcc cttcgagccg
     1861 gtcttgacgc gcacgggagg tgccgcccga acgtaaggct cgctggtttc accgcggatc
     1921 gtggccgcga tctgccgtgc ctgacgtgca atcggctcga tgaagcgcag ggcttcgccg
     1981 ccgatgctga tgcagtctcc cagcgcgtga atcgtggcaa cgtcggtgtg cagcgtgagc
     2041 gggtccaccg caatgccgtt gttccagctc agtcccgccg actgagccag gcggcttgga
     2101 gttcgcagcc cgacggcggc gatgacgtgc tcggcttcga attgccgccc gtcctgggcg
     2161 ctgaccgtcc gagaacttcc gctggcacgg acgccggtga tgtcgactcc ccccacgaag
     2221 gtgagcggca ggtcccgcca cgcggccagg agctcgttcg attgcgcctc ggtgagcaga
     2281 ctgctcagtg gtcgctcgca caggtcgagc agcgtcaccg gatagcccgc cagcgccaga
     2341 tcgttcgcca gctcgcagcc gaccaggccc gcgccggcga tcagcacacg ttgcggaggc
     2401 agcgctccgg gctcgccgag ccggtcgcgg aaattcagat acgcgtcgag gtggttgatg
     2461 cgccagcaca gcgcgggcgg caggttcggc agatcgatcg gatcggcgcc atgcgcaagc
     2521 accagatggc ggtagcgcag cgttccgcgc gtggtgcgca aggcccgcgc gccgacggac
     2581 acgctgatgg cctgcgtgcg tgccagcagc ctcacgccca gccgggccgc cgcttgctcc
     2641 gccgactccc tgaccagcga cgccggggcg atgcccttgg ccagcgccac cgacagcagc
     2701 ggcttgtcgt aaacattgcc gggacaggcg gtgacgatcg tgatcggcag atgcgcgtcg
     2761 tgctcgcgaa gcgactgagc catttgccag cccgcgcggc cggcaccgac gatgacgacg
     2821 ccggcatcgc gacgacctcc gcgagcgtgt ccggcagaag cggccgcact cgcggaggta
     2881 cgacgcaaca ccggcagcgg ctcgcagggt tcgaaatcag ccttgctgac gccacacagc
     2941 ggacacgccc agtcgtccgg tatgtcttcg aagcgcgtgc cgggaggcag gccaccgtcg
     3001 gcatcgccca gggcctcgtc gtagatccag ccgcaggcac ggcagatgaa ggtccgccag
     3061 gcttgcgcgg tcatgggttg cccgctgtgc tcatgggatc ccgcggccgg ccactcaagc
     3121 ctgcgccacg gtggcgctga gccgtgcgat ttccttgcgc aggtgcttga tcgccggcgt
     3181 gacgatggcc acgaagtgcg attcgcgcac acgccgctgc acggccgaac tcatcaggta
     3241 cccgcgtgcg ccatggtgca acagtgccga ctgcgccgcg cgcaaagcca gctcggcagc
     3301 gtgagcgcgt gtatcgcaaa cgtcgatcag gtattcggtg tcagtcgcga acggggtgcg
     3361 ggccagtcgc atcgtgcgct cggtcagggc gtcgagctcg gcttgcagct catcggggcg
     3421 ttcatcgagg aattcgttga cgtggccgag cgcgcgctcg acatcccaca tggagtcgat
     3481 ggcaccttga gtcacgccca gtcccatgcc gcactgcaac aggatgaacg ccgcacgcac
     3541 tcggccgatg taaggccgcg ccgggtctgc gacgagcgtg tcggcaccga tgaagtgatc
     3601 ggtcagtcgc accgcccacg tgttggtgcc ctccatggcc gagaacgacg ggcagttgcg
     3661 cagttccacg ccttcggcgt cgcagcgcac gatgaacatg atctcgtggc cgctggcttc
     3721 actggtggcg ctgtcggtgt tctcgacggt cgccaccgca ccgaagtagt ggtcgggccc
     3781 gaggttgctg acccagggca gggtgccgtt gacgcggtag ccgccttcga ccttgcgggc
     3841 atgcaacaga aaggtttcga tgcctgcgaa ggtcttcatc gggttgctca ggccggtggc
     3901 gcccagcgtg tggccggacg catggtcgcg cagcgcctga cccatcagcg ccgggttgcc
     3961 tgactcttcc atgtacaggc cgcaggcgtc gtgacaccac accatgaaac cggtggcgcc
     4021 gcagacccgg gaaacctcgg ccatggcctg gatggccgca acgtagtcat gctggccatc
     4081 gagccgcaag tgggcgctca gggcgcccgc cgcaccgagt tgcttcaaca ccgccttcgg
     4141 gtaggtacct tgccggtcga tggcgtcggc cattgccgcg aggtctccaa cggcaatcgc
     4201 ccgcaccttc tccagcatcg acgccttgat cgctgccgta ggagtcacct ggccagccag
     4261 aggctccagc gtcgaatcgg tgagcacggc tgccatgggg gggctcctga aaggtcaacg
     4321 cgggatcagg ccagatcgat ctggaacggg atcgggttgc gcatgctgtt ggccacaggc
     4381 gaata
//
LOCUS       DAOORV010000251         4370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_23809_length_4370_cov_5.964542, whole genome shotgun sequence.
ACCESSION   DAOORV010000251 DAOORV010000000
VERSION     DAOORV010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23809_length_4370_cov_5.964542"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..633)
                     /locus_tag="PKV56_18465"
                     /pseudo
     CDS             complement(139..633)
                     /locus_tag="PKV56_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
     gene            complement(656..1000)
                     /locus_tag="PKV56_18470"
     CDS             complement(656..1000)
                     /locus_tag="PKV56_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HOX69836.1"
                     /translation="MSLPALHNLSPDVCATAQLDTAAMAHLAQAGFKSVINNRPDHEG
                     GPDQPTSATLEGAATAAGLAYAHLPVAPGYQSPEEAAQFRSLLDTLPRPILVFCRTGT
                     RSGKLFQAAMSI"
     gene            complement(1026..1472)
                     /locus_tag="PKV56_18475"
     CDS             complement(1026..1472)
                     /locus_tag="PKV56_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOX69837.1"
                     /translation="MTLKDQINEDMKTAMRAKDAPRLLTIRGLLAACKQREVDERIVL
                     DDAAVIAIIDKLIKQRKDSISQFGAAGRSDLVDKETAELKVLEGYLPQRLSAEEIAAR
                     VQAIVIELGAAGPGDMGKVMAAVKTQLSGQADMGLVSAAVKQALAR"
     gene            complement(1573..1785)
                     /gene="rpsU"
                     /locus_tag="PKV56_18480"
     CDS             complement(1573..1785)
                     /gene="rpsU"
                     /locus_tag="PKV56_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOX69838.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTDLRAREFYEKPTAE
                     RKRKKAAAVKRHFKRVRSMQLPKKLF"
     gene            complement(1969..3168)
                     /locus_tag="PKV56_18485"
     CDS             complement(1969..3168)
                     /locus_tag="PKV56_18485"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX69839.1"
                     /translation="MQQFDAVIIGAGAAGLHCAAIAGQRGARVLLIDHFERVAEKIRI
                     SGGGRCNFTNRDTTPAQFLSDNPAFCRSALARYTPQDFISLLQRHRIAFHEKHKGQLF
                     CDESSEEIIAMLLRECEAGAVSRWQPCAVNEVTRTANGFELATGRGAVSTTRVIVATG
                     GLSIPKIGASDFGHRLARQFGHTIVEPRPALVPLTFDAAAWTPFVPLAGLSLEAIVAT
                     GTGKKRGEFTEDLLFTHRGLSGPAILQISSYWRPGTPLDLDLVPSHDLREALATAKTN
                     SRRKLSNELAEWLPRRLADAWLGSHPDIPDRAMPEVRDRDLATLATSLKQWKMVPSGT
                     EGYRKAEVTAGGVDTRELSSTSLESRRVPGLHFIGEVVDVTGWLGGYNFQWAWASAAA
                     CADSIGG"
     gene            complement(3204..4025)
                     /locus_tag="PKV56_18490"
     CDS             complement(3204..4025)
                     /locus_tag="PKV56_18490"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOX69840.1"
                     /translation="MPNRTVFFVSDGTGITAETFGNSILNQFSFRPHHVRRPFIDTVD
                     KAHQVMREINHAAEVEGRRPIVFVTLIDPAVQAIIKEHSNGLVLDLFNTFIEPLEAEF
                     GVKSNHRVGRFSDVSKSQEYTDRIEAINFSLAHDDGQSSKNLLSADVILVGVSRSGKT
                     PTSLYLAMQHGIKAANYPLIPEDFDRGCIPSSLAAYKKKCFGLTIDPVRLNQIRTERR
                     PDSKYASLENCRYEVREAETMMRREGIAWLSSTHKSIEEIATTILRDIRPDRLMY"
BASE COUNT          776 a         1362 c         1426 g          806 t
ORIGIN      
        1 aagcaaccgg gaagccgatc agcaggaaga ccaccagcga gccgaacatc cagggtgcca
       61 tgttggcggc gagcgactcg agcatcagac ctcgcctgtc tcggaggagg gcgtcggcag
      121 acccgggtca gtcgaggcgc cgcgcaggaa agcgacgcgc ttgatcagtt cggacacgcc
      181 ttgcgcgccg agcagcgcga agccgagcgg taaaagcatc agcaccggcc agcggatcag
      241 gccaccggca ttgctcgaca tctctgcgct ggcgtaagcc cgggccgcca gcggccacga
      301 caggccgatc accacgatga ccaggggcat caggaaaaag acgatgccga aaatgtcgat
      361 ccaggcccgt gatctctcgg acaggcgcgc ggtgagcacg tcgatgcgaa cgtgctcctg
      421 gcgcagcagc gtgtgcccgg ctgccagcag gaagaccgcc gagaacaggt accactggat
      481 ctcgaggaat gcattcgagc tggtgtttta cagcttgcgg acgatcgcat tgcccgcgct
      541 gaccagtacc gctgccagca ccagccaggc gacaccgcgt ccaacggcct cgttgagcct
      601 gtcgatgtac cgcgacagcg caagcacgtt cagtgagccg acgcgccggc tgtgctcaga
      661 tggacatcgc cgcctggaac agcttgcctg agcgcgtccc ggttcggcag aacacgagga
      721 tcggccgcgg cagcgtgtcg agcaggctgc ggaactgcgc cgcttcctca ggactttgat
      781 agccgggagc gaccggcagg tgggcgtagg cgaggccggc cgcggtggcc gcaccttcga
      841 gcgtggcgct ggtcggctgg tcggggccgc cctcgtgatc gggccggttg ttgatcacgc
      901 tcttgaatcc ggcctgagcc aggtgggcca tcgctgcggt gtcgagctgg gcggtcgcgc
      961 agacatcagg gctcaggttg tggagcgcag gcaggctcat ggatgttcct tcgtctggag
     1021 tcggctcaac gggccagagc ctgcttcacg gccgcggaaa ccagccccat gtcggcctgg
     1081 ccggagagct gagtcttgac cgcagccatc accttgccca tgtcgccggg gcccgctgcg
     1141 ccgagttcga tcacgatggc ctgcaccctg gccgcgattt cctccgcgct caggcgctgt
     1201 ggcaggtagc cttcaagcac cttcaactcg gcagtttcct tgtcgaccag atcggagcga
     1261 ccggctgctc cgaactgcga gatcgaatcc ttgcgctgtt tgatcagctt gtcgatgatg
     1321 gctatcaccg cggcgtcatc gagcacgatg cgctcatcga cctctcgctg cttgcacgcg
     1381 gccagcagcc cgcgaatcgt cagcaaccgg ggcgcatcct tggcgcgcat ggccgtcttc
     1441 atgtcctcgt tgatctggtc tttcagtgtc atggtgcgct cagtcggtgg gaatcaaggg
     1501 aaaagccggc agcgtaatta acaaagcccg cacggagcgg gctttggggc tattcgacgt
     1561 gcgaagtggc tgtcagaaca gctttttcgg caactgcatg ctgcgaacgc gcttgaagtg
     1621 gcgcttgacc gctgcagcct tcttgcgctt gcgctcggct gtgggctttt cgtagaactc
     1681 gcgagcccgc aggtcggtca gcaagccgag cttctcgatg gtgcgcttga agcggcgcag
     1741 cgccacgtcg aatggctcgt tttctttcac gcgaatcgta gtcatgtatc gattgaatcc
     1801 agattgaatg tgcttgaagc gggttgctcg acatcgaaac ggaaggtccg gactctcgag
     1861 tcgcgcaagc aaaccaccgt caagcgtgtt tgccagcaaa gcgcgagatt atagacagcc
     1921 atttccatac tggcaatgtt gccagccagg aaaccgcggg ctgcgtgctc acccgcctat
     1981 cgaatcggca caggccgccg cgctcgccca ggcccactgg aagttgtacc cccccagcca
     2041 gccggtcaca tcgaccacct cgccgatgaa atgcagcccg ggaacacggc gactttcaag
     2101 tgatgtggag ctcagctcgc gggtgtcgac gccgcctgct gtcacctcgg ccttgcggta
     2161 gccctcggtt ccgctgggca ccatcttcca ttgcttcagg ctggttgcca gggttgccag
     2221 atcgcggtcg cgcacctcgg gcatggcccg gtccgggatg tcggggtggc tgcccagcca
     2281 ggcatcggcc aaccgacgcg gcagccattc ggcgagttcg ttggagagct tgcgcctcga
     2341 gttcgtcttg gctgtcgcca gcgcttcccg caagtcgtga ctggggacca gatccaggtc
     2401 gagcggcgtt cccggacgcc agtagctcga gatctgcagg atggccggac cgctcagccc
     2461 gcggtgcgtg aagagcagat cctcggtgaa ttcgccacgt ttcttcccgg tgccagtggc
     2521 cacgatcgcc tcgagcgaca gcccggccaa gggcacgaag ggcgtccagg ccgcagcgtc
     2581 gaaggtcagc ggaaccagcg caggccgggg ctcgacgatg gtgtggccga actgacgcgc
     2641 cagtcggtgg ccgaagtcgc tggcgccgat cttcggtatc gacagtccgc cggtggcaac
     2701 gatcacgcgt gtcgtgctca ccgcaccgcg gccggtggcc agttcaaacc cgttcgccgt
     2761 ccgggtcact tcattgaccg cgcagggctg ccatcgagac acggcgcctg cctcgcactc
     2821 gcgcaacagc atcgcgatga tttcctcgct cgattcgtcg cagaacaact ggcccttgtg
     2881 cttctcgtgg aacgcaatgc ggtgccgctg cagcagcgag atgaagtcct gcggcgtgta
     2941 gcgggccagc gccgaccggc agaaggccgg gttgtccgac aggaactgcg ctggcgtggt
     3001 gtctcggttg gtgaagttgc agcgcccgcc gcccgagatg cggatcttct cggcgacgcg
     3061 ctcgaagtga tcgatcagca gcacgcgcgc tccgcgctgg ccggcgatcg cggcgcagtg
     3121 cagcccggca gcgccggctc cgatgatcac cgcgtcgaat tgctgcatcg gcgcggatga
     3181 tacgagccgc tgggggctcg acttcagtac atcaggcgat cggggcggat gtcccgcagg
     3241 atggtggtcg cgatctcctc gatcgacttg tgtgtcgacg acagccacgc gatgccttcc
     3301 cggcgcatca tggtctcggc ttcccggacc tcatagcggc agttctcgag cgaggcgtac
     3361 ttgctgtcgg gtcgcctctc ggtgcggatc tggttcaggc gcaccggatc gatcgtcagg
     3421 ccgaagcact tcttcttgta ggcggccagg ctggatggaa tgcagcctcg atcgaagtcc
     3481 tcgggaatca gcgggtagtt cgcggccttg atgccgtgct gcatggccag gtacagcgag
     3541 gtcggcgtct tgccgctgcg gctgacgccg accaggatca cgtcggccga cagcaggttc
     3601 ttggacgact ggccatcgtc gtgggccagg gagaagttga tcgcttcgat gcggtccgtg
     3661 tactcctggc tcttggacac atcggaaaac cggccgaccc ggtgattgga cttgacgccg
     3721 aactcggcct ccagtggctc gatgaaggtg ttgaaaaggt ccagcaccag gccgttgctg
     3781 tgttccttga tgatggcttg cacggccggg tcgatcaggg tgacgaagac gatgggccga
     3841 cggccttcga cttccgcggc gtgattgatc tctcgcatca cctggtgagc cttgtccacg
     3901 gtgtcgatga acgggcgtcg aacatggtgc ggcctgaacg agaactggtt caggatcgaa
     3961 ttgccgaagg tctcggcggt gatgccggtg ccgtcggaga cgaagaagac tgtgcggttt
     4021 ggcatctagg cagacctatc ggttgaatga ccagccacat catagggatt caaccgtaaa
     4081 ctcgatttca gttttcccgc acagccaccg acaggcggac atgcccaccc acccaccatc
     4141 gctcgacgct gctgcacgca agacgcactg cgatggtccg cggccctcga agcgaatccg
     4201 ctcgggctgc atcaaggcat ggcgggacgc accagttttt tcacatcacg gagctatttc
     4261 atgtcaaccc agaacctggc ggccgccctg gtcgttccct ttgaagaatt gaggatgtcc
     4321 gacgtcgagt cggtaggcgg caagaacgcc tcgctcggcg aaatgatcag
//
LOCUS       DAOORV010000252         4350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_23946_length_4350_cov_13.808382, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000252 DAOORV010000000
VERSION     DAOORV010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23946_length_4350_cov_13.808382"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKV56_18495"
     CDS             complement(<1..216)
                     /locus_tag="PKV56_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69841.1"
                     /translation="MTDETRSTCPYCGVGCGVIIESDGAQITGVRGDPEHPANFGRLC
                     TKGSTLHLTAAQPITMQARLLQPQMRDS"
     gene            complement(213..647)
                     /gene="nirD"
                     /locus_tag="PKV56_18500"
     CDS             complement(213..647)
                     /gene="nirD"
                     /locus_tag="PKV56_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOX69842.1"
                     /translation="MTPAAPGWTAVCAVDDIPPLGARRVARPQGMAVAVFRTADDRVF
                     ALLDRCPHKGGPLSQGIVFGNSVACPLHNWTIGLTDGCAQAPDVGCTVPFAVKVEAGQ
                     VYLDASELAGLALDLQAPRAGPGASTGDLGRSPLDAPRPHSP"
     gene            complement(668..3154)
                     /gene="nirB"
                     /locus_tag="PKV56_18505"
     CDS             complement(668..3154)
                     /gene="nirB"
                     /locus_tag="PKV56_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HOX69843.1"
                     /translation="MKKMKLVMVGNGMAGVRTLEELLKIAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLDEIVLNPLDWYSDNHITLHTGKKVVHIDRRQRIVRAVDGNGAET
                     EAEYDRLLMATGSNPFILPVPGKDLEGVIGYRDIADTNAMIDAATKYQHAVVIGGGLL
                     GLEAANGLMLRGMQVTVVHIAPWLMERQLDDVAGKLLQKSLEDRGLKFLIGAQTQALI
                     GDRDGGKSGRVMAVQFKDGTEIPADLVVMAAGIRPNTELAIKIGLYCGDNNRSGLVVN
                     DTMQTITDPRVYAVGECAAHRGTVYGLVAPLFEQGKVCATHLAQFGIGRYLGSQTSTK
                     LKVTGIDLFSAGNFTGGADFEEIVMSDPYAGVYKKLVIQGDKLVGACLYGDTVDGSWY
                     FKLLREGRKIGDIRDKLMFGESNIGDVGHQGHSKAAAMADADEVCGCNGVTKGAICKA
                     IKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYSKAPTKKAMCGCTDASH
                     QEAREAIKADHLLTIDSVYKRLGWRTPNGCASCRPAINYYLISSWPKEAKDDPQSRYI
                     NERSHANIQKDGSYSVIPRMWGGETTADELRRIADAVDKYKIPTVKVTGGQRIDLLGV
                     KKEDLPGVWKDIGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTQMGKDLERAMWRMY
                     APHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYVAGNGGIKTEVAQFFTKLKTPE
                     EVLEYTGAFCQLYREEGWYLERTVHYVHRVGLDYVKKKVLDDHEGRRALWAKLQFSLD
                     GEPDPWFEHAKAQVDLRQFSAITPSLPVSA"
     gene            3954..4184
                     /locus_tag="PKV56_18510"
     CDS             3954..4184
                     /locus_tag="PKV56_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69844.1"
                     /translation="MKSFQFLRKMHRTGALASEAAPSTVNSGFHDSKPMQPKPDPRGW
                     FDSSEDLRGGLHVQEEDFDSLPEEFRDVFSSR"
BASE COUNT          696 a         1461 c         1412 g          781 t
ORIGIN      
        1 actgtcgcgc atctgcggct gaagcaagcg cgcctgcatc gtgatcggct gcgccgccgt
       61 caggtgcaac gtgctgccct tggtgcacag gcggccgaag ttggccggat gctcggggtc
      121 accgcgcacg ccggtgatct gcgcaccgtc gctctcgatg atcacgccac agcccacacc
      181 gcagtacggg caggtggagc gcgtttcgtc ggtcatgggc tgtgaggcct cggtgcatcg
      241 aggggcgacc tgccgaggtc tccggtggac gctccagggc cggctcgggg agcttgcaga
      301 tcgagcgcaa gcccggccag ctccgatgca tccaggtaga cctgacccgc ctcgaccttg
      361 accgcgaacg gcaccgtgca acccacgtcc ggagcctgcg cgcatccgtc ggtgaggccg
      421 atcgtccagt tgtgcagcgg gcaggcgacg ctgttgccga acacgatgcc ctggctcagc
      481 gggccgccct tgtgcgggca gcggtcgagc agggcgaaca cccggtcgtc ggcggtgcgg
      541 aacaccgcca ccgccatgcc ctgcgggcgg gccaccctgc gtgcaccgag cggggggatg
      601 tcgtcgaccg cgcagacagc ggtccaaccg ggggcagcgg gggtcatggg caggttctcg
      661 cggtgcatca cgcgctcacc ggcaggctgg gggtgatggc gctgaactgc cgcaggtcga
      721 cctgggcctt ggcatgctcg aaccaggggt cgggctcgcc gtccaggctg aactgcagct
      781 tcgcccacag cgcacgccgg ccctcatggt cgtcgaggac tttcttcttc acgtagtcca
      841 aaccgacccg atgcacatag tgcacggtgc gctccaggta ccagccttcc tcgcggtaga
      901 gctggcagaa ggcgccggtg tactccagca cctcctcggg cgtcttcagc ttggtgaaga
      961 actgcgccac ctcggtcttg atgccgccgt tgccggccac gtacatctcc cagcccgagt
     1021 ccacgccgat gatgcccacg tccttgatgc cggcctcggc gcagttgcgc gggcagccgc
     1081 tgacggcgaa cttcaccttg tgcggcgcgt acatgcgcca catggcgcgc tccaggtcct
     1141 tgcccatctg ggtgctgtcc tgggtgccga agcggcacca ctcgctgccc acgcaggtct
     1201 tcaccgtgcg cagcgccttg gcataggcat ggccgctggg catgccgatg tccttccaca
     1261 cgcccggcag gtcttccttc ttcacgccca gcaggtcgat gcgctggccg ccggtgacct
     1321 tgacggtcgg gatcttgtac ttgtcgaccg cgtcggcgat gcggcgcagc tcgtctgcag
     1381 tggtttcgcc gccccacatg cgcgggatca cgctgtagct gccatccttc tggatgttgg
     1441 cgtggctgcg ctcgttgatg tagcggctct gcgggtcgtc cttcgcctcc ttcggccagc
     1501 tgctgatcag gtagtagttg atcgccggcc ggcaggaggc gcagccgttg ggcgtgcgcc
     1561 agcccaggcg cttgtagacg ctgtcgatgg tcagcaggtg atcggccttg atcgcctcgc
     1621 gcgcctcctg gtggctggcg tcggtgcagc cgcacatcgc cttcttggtc ggcgccttgg
     1681 aatagtcgcc accggccgtg aacatcagca actgctcgac cagcccggtg caggagccgc
     1741 agctcgcgct ggccttggtg tgcttgcgca cctcgtcgag ggtgaacagg cccttctcct
     1801 tgatcgcctt gcagatcgcg cccttggtga caccgttgca tccgcagacc tcgtcggcat
     1861 cggccatcgc agctgccttg ctgtggccct ggtggccgac gtctccgatg ttcgattcgc
     1921 cgaacatcag cttgtcgcgg atgtcgccga tcttgcggcc ctcgcgcagc agcttgaagt
     1981 accaggagcc gtcgacggtg tcgccgtaaa ggcaggcgcc gacgagcttg tcgccctgga
     2041 tcaccagctt cttgtagacg ccggcgtacg ggtccgacat cacgatctcc tcgaaatcgg
     2101 cgcctccggt gaagttgccg gccgagaaca ggtcgatgcc ggtcaccttg agcttggtcg
     2161 aggtctgcga acccaggtac cgaccgatgc cgaactgcgc caggtgggtc gcgcagacct
     2221 tgccctgctc gaacagcgga gccaccagcc cgtacaccgt gccgcggtgt gcagcgcact
     2281 cgccgaccgc atagacacgc gggtctgtga tggtctgcat ggtgtcgttg acgaccagcc
     2341 cgctgcggtt gttgtcgccg cagtagagac cgatcttgat ggcgagctcg gtgttgggtc
     2401 ggatgccggc ggccatcacc accaggtcgg ccggaatctc ggtgccatcc ttgaactgca
     2461 ccgccatcac gcggccggac ttgccgccgt ccctgtcgcc gatcagcgcc tgcgtctgtg
     2521 caccgatcag gaacttcagg ccgcggtcct ccagcgattt ctgcagcagc ttgcccgcga
     2581 cgtcgtcgag ctggcgctcc atgagccacg gcgcgatgtg caccactgtc acctgcatgc
     2641 cgcgcagcat caggccgttc gccgcctcga ggccgagcaa cccgccgccg atgaccacag
     2701 cgtgctgata cttggtcgcc gcgtcgatca tcgcgttggt atcggcgatg tcgcggtagc
     2761 cgatcacgcc ttccagatcc ttgccgggca ccggcaggat gaacgggttc gacccggtgg
     2821 ccatcagcag gcggtcgtat tcggcttcgg tctcggcgcc atttccgtcc accgcgcgaa
     2881 cgatgcgctg cctgcggtcg atatgaacca ccttcttgcc ggtgtgcagc gtgatgtggt
     2941 tgtcgctgta ccagtccagc gggttcagca cgatctcgtc gagcgtctgc tcgcccgcaa
     3001 gcaccggact cagcaggatg cggttgtagt tcgggtgcgg ctcggcgccg aacacggtga
     3061 tgtcgtagag gtcaggcgcg atcttcagca gctcctcgag cgtacgcaca ccggccatgc
     3121 cgttgcccac catcacgagc ttcatcttct tcatggctta cgcctccagg cgggggatcc
     3181 cgcattcagt cggaaaaatg gaataaaccg tcacccgagg ccgcgctgcg gtgggctcgc
     3241 acagggctcg aaaggggcga tggacgcagc agccgacgcc catcgaatcc aatggcgtga
     3301 aaggcgcagt tcgagcgttc gccagattca aggcgtgacg catggggtgt ggcgtaacga
     3361 aacgcgggcg acgcagacca tgacgggctc ccaggctcgc cgcggaaaaa tgccgcggcg
     3421 cggaaataca tcaccacgca cttcgtcgtg cgcgcgaccc gccatgtact tatgcagggt
     3481 cgggtgcgtc gctgcacccg cctcaacctc agcaagtagc gggccagaca ggtgaacagc
     3541 ctatcgaccg gctccgtcct ccgggtctgc agaccgggct ttcgccgtgt tggacggacc
     3601 cggtcacgag gatggtgcgc gacgccgtgc cgagatggtg cgccgagcga tccaggccgg
     3661 cacgagagcg aacgtcccca tgctttcccg gcaccgtcac aacccagtac aaaggcaact
     3721 ggtggcctgc acgaggtccg caccgaacac cgaggggacg gtcacccatg catttccgac
     3781 cgagaaacca tgctcgaccg tgaatagtgc tcgccgcgcc agttcgaaaa cgccgccacc
     3841 actcagtcac ggggtgacgt caccggcccg accggactaa attttgcgtg tgtcgagatg
     3901 tttctgatgg cggctcgcca tcgacagatt tgcatcgatc aggggcccga cgaatgaaat
     3961 ccttccagtt cttgcgcaag atgcaccgaa ctggcgcgct cgcgtccgaa gcggccccat
     4021 cgaccgtgaa cagcggattc cacgacagca aaccgatgca gccgaagccc gatcctcgcg
     4081 gctggttcga ctcgtcggaa gacctcaggg gcggcctgca cgtgcaggaa gaagacttcg
     4141 attcacttcc cgaagaattc cgcgacgtct tctcctcacg ctgatccgat cgcttcccgg
     4201 tagagggcca ggtactgctg cgccgtcggc caccgcgcgg gctgctgggc gggatggtgg
     4261 agctgccggg cacaccgtgg cgcgaggccg tctggggggc cgaagaggca tttgcccatg
     4321 cgccgcaacc cgctgactgg cgcccggccg
//
LOCUS       DAOORV010000253         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_24300_length_4302_cov_5.856134, whole genome shotgun sequence.
ACCESSION   DAOORV010000253 DAOORV010000000
VERSION     DAOORV010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24300_length_4302_cov_5.856134"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKV56_18515"
     CDS             <1..302
                     /locus_tag="PKV56_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOX69845.1"
                     /translation="GMRSTVTAAPGFAPHINGTGNGSLAGGGTGDVLAGWIGGLWAQA
                     QRHRDEDELGIAFATASRAVAEHGAAADPLQPGALPASELIRRLHHGLRMGHPAE"
     gene            complement(333..2564)
                     /gene="rnr"
                     /locus_tag="PKV56_18520"
     CDS             complement(333..2564)
                     /gene="rnr"
                     /locus_tag="PKV56_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOX69846.1"
                     /translation="MSEVVGTVQGHRDGHGFVIRDDRQPDLYLSQQEMRAVLHRDRVK
                     ARIVRFDRKGRPEGRVLEIIERKKAPIIGRLLHESGVWLVAPEDKRYGQDILIPKNAV
                     ANATAGQVVAIELTEPPSMFSQPVGRVTEVLGEIDDPGMEIEIAVRKYEVPHRFSPEA
                     LAQAAGLPDRIRAIDRKGRIDLTDVALVTIDGEDARDFDDAVYCEPAKVGRAKGWRLV
                     VAIADVSHYVKPGEALDNDAYERATSVYFPRRVIPMLPEKLSNGLCSLNPNEDRLAMV
                     CDMLITATGEIYAYQFFPAIINSHARFTYNEVAAILGNTRGPEASRRKDLVDDLLNLN
                     EVYRALVKERGKRGAIDFETTETQIVCDENGRIEKIIPRTRNEAHKLIEESMLAANVC
                     AADFISGSKHPSLYRVHEGPTPEKKTLLQNYLKALGLGLSIGDDPHPREYQQIAAATK
                     DRIDAEQIHSMLLRSMQQAVYTASNSGHFGLAYDAYAHFTSPIRRYPDLLVHRVIKSI
                     LGGKRYLLSKGMVESMPLARSLSKPSKQAKTAKAVTLTAEGEVWEIAGAHCSANERRA
                     DEASRDVEAWLKCRYMREHLGEEYSGRVSAATSFGLFVQLDGLYVEGLVHITELGGEY
                     FRFDEVKQELRGERTGVRYAVGSRVRVQVSRVDLDGRKIDFRMVRDGEEDAAMARARK
                     ERGADRNASAVAELASVKETDRAVKAAGKRKGYSASGTRVNPGRAAKTPARKGPAKAA
                     KRR"
     gene            2637..2721
                     /locus_tag="PKV56_18525"
     tRNA            2637..2721
                     /locus_tag="PKV56_18525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2671..2673,aa:Leu,seq:cag)
     gene            complement(2836..3357)
                     /locus_tag="PKV56_18530"
     CDS             complement(2836..3357)
                     /locus_tag="PKV56_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOX69847.1"
                     /translation="MLTDDGKDLYVSWGEYHMLIERLALKIHASGWEFDQILCLARGG
                     MRPGDVLSRVFDKPLGIMATSSYRASGGTLQGRLDMATYITLPQGELGGRVLLVDDLA
                     DTGVTLRAVVERLRGMPSISELRSAVIWTKGVSSYVPDYHVELLPTSPWIHQPFEEYD
                     GMRPDVLAKKFAI"
     gene            complement(3395..>4302)
                     /locus_tag="PKV56_18535"
     CDS             complement(3395..>4302)
                     /locus_tag="PKV56_18535"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOX69848.1"
                     /translation="YEDKVARRALRVQDLKHPERFARKLQELLDLHNVALSGYLRSKP
                     LEFQPIFDHAMSVAEQIKPMMADVGYSLHTAHRAGANLLFEGAQGTLLDIDHGTYPYV
                     TSSNCVAGNASAGAGVGPDLLHYILGITKAYTTRVGSGPFPTELSMDVPGTVGHHLST
                     VGQERGTVTGRPRRCGWLDAAALKRSIIINGVSGLCITKLDVLDGLEEIKVCVGYELQ
                     GRRIDILPLDADDIAACVPIYDSFPGWSETTAGVTQWSQLPLNARRYLERVQSFIGAP
                     IDMVSTGPDRDHTILLRHPYQMQSA"
BASE COUNT          784 a         1338 c         1353 g          827 t
ORIGIN      
        1 cgggaatgcg cagcacggtc acagcggcgc cgggattcgc gccacatatc aacggcacgg
       61 gcaacggctc actggccgga ggtggcacgg gggacgttct ggcaggctgg atcggtgggt
      121 tgtgggccca ggcacaacgc caccgggatg aagacgagtt gggcatcgct ttcgccaccg
      181 catcacgcgc agtggccgag cacggtgcgg cagccgatcc cttgcaacct ggcgcactgc
      241 cggccagcga attgataaga cgactgcacc acggtttgcg catgggccac cctgccgaat
      301 gacccgacgc ggttccaacg gcgcctgacg gatcagcgcc gtttggctgc cttggctggt
      361 cccttgcgcg ccggcgtctt ggcggcacgc cctggattca cccgggtccc cgatgcgctg
      421 taccccttgc gttttccagc agccttgacc gcgcgatccg tttccttgac gctggccagt
      481 tcggccactg cactggcatt gcgatcggcg ccccgctctt tacgggcgcg agccatcgcg
      541 gcatcttcct cgccatctcg caccatgcgg aaatcgatct tgcggccgtc gagatcgact
      601 cggctgacct gaaccctgac acgcgaaccg acggcataac gcaccccggt gcgctcgcca
      661 cgcagttcct gcttgacctc gtcgaagcgg aaatactcgc caccgagctc agtgatgtgc
      721 accaaacctt ccacgtacaa cccgtcaagc tgcacgaaga gcccgaaact cgtcgccgcg
      781 ctgacgcgac cgctgtattc ctcgccgaga tgctcgcgca tgtagcggca cttcagccag
      841 gcttccacgt cgcgcgaggc ctcatcggct cgacgttcgt tggcactgca gtgcgcgccc
      901 gcaatttccc atacctcgcc ttcggccgtc agagtcacgg ccttggctgt cttggcttgc
      961 ttggacggtt tcgaaaggct gcgcgccagc ggcattgatt cgaccatgcc cttgctgagc
     1021 aggtagcgct tgccgccgag gatggacttg atgacccggt gcaccagcaa gtcgggatag
     1081 cggcggattg ggctggtgaa gtgcgcgtaa gcgtcgtaag ccagaccgaa gtgtccgctg
     1141 ttgctggcgg tgtaaacggc ctgctgcatc gagcgcagca gcatcgaatg gatctgctcg
     1201 gcatcgatgc gatccttggt agccgccgcg atctgctgat actcgcgcgg gtgcggatcg
     1261 tccccgatcg acaagccgag accgagtgcc ttcaggtagt tctgcagcag cgtcttcttc
     1321 tcgggtgtcg gtccttcgtg cacacggtac agcgaaggat gcttcgagcc ggagatgaaa
     1381 tcggcagcgc agacgttggc agccagcatc gactcctcga tcagcttgtg cgcttcgttg
     1441 cgggtccgag gaatgatctt ctcgatgcgg ccgttctcgt cgcagacgat ctgcgtctcg
     1501 gtggtctcga agtcgattgc gccgcgcttg ccgcgttcct tgaccagcgc gcggtagacc
     1561 tcgttgaggt tcagcagatc atcgaccaga tccttgcgcc gcgaggcctc gggcccgcgt
     1621 gtgttgccaa gaatggcagc cacctcgttg tacgtgaacc gcgcgtgcga gttgatgatg
     1681 gccggaaaga actggtaagc gtagatctct ccggtggcgg tgatgagcat gtcgcagacc
     1741 atcgccagcc ggtcttcatt cgggttcagg gaacacaggc cgttggacag cttctcgggc
     1801 agcatgggaa tcacgcggcg cgggaaataa acagaagtcg cgcgctcgta ggcatcgttg
     1861 tcgagtgcct cgccgggttt cacgtagtgg ctcacgtccg cgatcgcgac gacgaggcgc
     1921 cagcctttcg cacggccgac cttggcaggt tcgcagtaca ccgcgtcgtc gaagtcgcgc
     1981 gcgtcctcgc catcgatggt gaccagcgcg acgtcggtca ggtcgatgcg acccttgcga
     2041 tcgatcgcgc gaatcctgtc cggcagtccg gcagcctgcg ccagcgcttc gggcgaaaag
     2101 cgatgtggca cctcgtactt gcgcaccgcg atctcgattt ccatcccggg atcgtcgatc
     2161 tcgccgagca cctcggtgac gcgaccaacg ggctgcgaga acatcgaagg tggctctgtc
     2221 aactcgatcg cgacgacttg ccctgccgtg gcgttggcca ccgcattctt cgggatcagg
     2281 atgtcctgtc catagcgctt gtcttcaggt gcgaccagcc agacgccact ttcatgaagc
     2341 agacggccaa tgatcggcgc cttcttgcgc tcgatgattt cgagcacgcg accttctggt
     2401 cggcccttgc ggtcgaaacg cacgatgcgc gccttgacgc gatcacggtg cagcaccgcg
     2461 cgcatttcct gttgcgacaa gtagagatcg ggctggcgat cgtcgcggat cacgaatccg
     2521 tgcccgtcgc gatgcccctg cacagtaccg acgacctcgc tcatgaggtc ggctccaacg
     2581 gagggggagg cttggtttat tgttttatcg atgatatgat ctcaggcttc ctgaatgccc
     2641 aggtggcgga attggtagac gcactagttt caggtactag cgggtaactc cgtggaggtt
     2701 cgagtcctct cctgggcacc aaatataacg aagccagctc acgctggctt ctttgttttg
     2761 gggcggcgat acacgccgcg atagggttga aaagacgatg tcgcgcagag gtcggaagtt
     2821 cgatcgaccc ggctctcaaa tcgcgaactt cttcgcgagc acgtccgggc gcatgccgtc
     2881 gtactcttcg aagggttggt gaatccaggg gctcgtcggc aacagctcga cgtgatagtc
     2941 gggcacatag gacgagacac ccttggtcca aatgaccgcc gagcgaagct cgctgatgga
     3001 tggcatgccg cgaagccgct cgaccacagc gcgcagcgtc accccggtgt cagcaagatc
     3061 gtcgacgagg agcactcgcc cgccgagttc gccctgaggc agcgtgatgt aggttgccat
     3121 gtcaaggcga ccctgcagag ttcccccgct cgcccggtaa gaactggtag ccatgatgcc
     3181 cagcggcttg tcaaagacgc gcgaaagtac atcacccggc cgcatgcccc cacgcgcaag
     3241 gcagaggatc tgatcgaact cccagcccga cgcatggatc ttcagtgcca gcctctcgat
     3301 gagcatgtgg tactcgcccc aggacacata aaggtccttg ccatcgtcgg tcaacatgca
     3361 ggctcctggc ggcaacgggt tacgtgagtc agcgtcacgc cgattgcatc tggtaaggat
     3421 ggcgcagcag gatcgtatgg tctcgatccg gcccggtcga aaccatgtcg atcggcgcgc
     3481 cgatgaagga ttggacacgc tccaggtaac ggcgggcatt cagcggcaat tgcgaccact
     3541 gtgtcacacc ggccgtggtt tcgctccaac cggggaacga atcgtagatg ggcacacagg
     3601 ccgcgatgtc gtcggcatca aggggcagga tgtcgatgcg ccgcccctgc agttcgtagc
     3661 caacgcagac cttgatctcc tccaatccgt cgagaacgtc gagcttggtg atgcacaggc
     3721 cagacacgcc attgatgatg atcgagcgct tgagggcggc tgcgtccagc cagccgcacc
     3781 ggcgcggccg gccggtgacg gtgccgcgtt cctgcccgac ggtggacagg tgatggccga
     3841 ccgttccggg cacgtccatc gacaactcgg ttggaaacgg accgctgccc acgcgcgtgg
     3901 tgtaggcctt ggtgatgccg aggatgtaat gcagcagatc cggaccgacg ccagcgccag
     3961 ccgaggcatt gccagcaacg caattgctcg acgtgacgta agggtaggtg ccgtggtcga
     4021 tatcgagaag cgttccctga gcgccttcga acagcaggtt ggcaccggcg cggtgagccg
     4081 tgtgcagcga atagcccacg tcggccatca tgggcttgat ctgctcggca accgacatcg
     4141 cgtgatcgaa gatcggctgg aattcgagtg gcttcgaacg caggtagccc gacagcgcga
     4201 cgttgtgcag atccagcaac tcttgaagct tcctggcgaa gcgctcgggg tgcttgaggt
     4261 cctgcactct caatgctcgg cgtgcgacct tgtcctcgta gg
//
LOCUS       DAOORV010000254         4215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_24953_length_4215_cov_6.176923, whole genome shotgun sequence.
ACCESSION   DAOORV010000254 DAOORV010000000
VERSION     DAOORV010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24953_length_4215_cov_6.176923"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..529
                     /locus_tag="PKV56_18540"
     CDS             164..529
                     /locus_tag="PKV56_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69849.1"
                     /translation="MGWTFVRQTRDQLIRELLAPQASERACCEVIDHTLDGDVLWTVV
                     RVTARQAGVMGLAAGESVCYIGCHLLESSGGDWGYKSLDESVHPYYYSCPLRYLDMAP
                     VQSSEWRERVHRFHAGRAV"
     gene            614..1264
                     /locus_tag="PKV56_18545"
     CDS             614..1264
                     /locus_tag="PKV56_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69850.1"
                     /translation="MPAPSSAKPLYRIDECPDLMADGCVGDEQGNLVFLSIWARDTAV
                     QEFLARLTLGRDEQGLDQFHVITEQGASIPVFVGNVENLEKRITRAYRRTLFGSLTNV
                     WLFDRRCVKPDKANASALALLPRDSAHRLDRLWTLVQDTCPLPLLDHWRDTVLELLQT
                     RRMLTGLPLALGPLEGHRLALDVPALTKALGELIRNGTLGATQYELAANAPLRRVA"
     gene            1330..2439
                     /locus_tag="PKV56_18550"
     CDS             1330..2439
                     /locus_tag="PKV56_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HOX69851.1"
                     /translation="MALMFPRLARNFIRNGYFPTDEPTLERALSALAPSPGSMSILDP
                     CAGEGVAIAEAAHALGREQVQAFAVEYDAERARHARQLVDRCIHGDLMDTLISRQSFG
                     LLWLNPPYGDLSKDVNGNIGYQGQGRARLEKLFYQRALPLLQYGGVLIFIVPSYVLDA
                     ELVGWLTRHFADLRIYRAVETQFKQVVIFGRRIRQRDQASESVKAVRGLLLQIGQGDA
                     EAEELPLEWPFLPYTVPASPAEPEHFYRVTMEPEQFADEVGRLQGLWPALDTHLGAAQ
                     QSLRPPARALSHWHLALALAAGAISGVVTSKSGRVLVVKGDTHKEKTLQTEYTERDDG
                     SVAETRILTDKFVPVIRAWDLTLGSPTWGEVLTIR"
     gene            2541..2846
                     /locus_tag="PKV56_18555"
     CDS             2541..2846
                     /locus_tag="PKV56_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69852.1"
                     /translation="MALAVLNLASQARFSPGQVVMTAGVDELVRQGRLNPTPYLRRHL
                     HGDWGDLDDSDRRQNDAALKSGEDRLFSSYQVTRDLKLWIITEWDRSVTTLLLPSEY"
     gene            2946..>4215
                     /locus_tag="PKV56_18560"
     CDS             2946..>4215
                     /locus_tag="PKV56_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX69853.1"
                     /translation="MSLDLETVPETAVQGDLLEAAASPLTLSLTDFVSEFGDELLDSL
                     NRANPPVYTGQVRVHRQLILAALKRKLFPAQADVVHAVTELLVDRGERAAIVNGEMGC
                     GKTTVGIATAAVLNAEGYRRTLVLSPPHLVYKWRREIQETVAGAKVWVLNGPDTLVKL
                     LKLREQLGVPAQGQEFFVLGRVRMRMGFHWKPVFVRRRTPHGDVGACPDCGHVITDLD
                     GEPINPVELEAEESRRKCSHCRAPLWSLIRPRGLSASDQSSTVLKALKRIPTIGEVTA
                     QKLMQKFGDAFLASMLGDNIHEFINLMDGNGELVFSDRQAHRMERAMANMEFGFGEGG
                     YQPSEFIKRQLPQGTFDLLIADEAHEYKNGGSAQGQAMGVLAAKARKTLLLTGTLMGG
                     YGDDLFHLLFRALPGRMIEDGYRPTTSGSMT"
BASE COUNT          692 a         1458 c         1325 g          740 t
ORIGIN      
        1 agcgcgaccg tctcgacaag ctgggctacg agtttgctcc gttgtcccag gactggcacc
       61 tcaaagctgc ctgatccatc cgccgccttg cgcggcatac cgccacccgc cggggctctc
      121 ccccgacggg gagggctccg gccttttctt caaggagacc ctcatgggct ggaccttcgt
      181 tcgtcagacg cgcgatcagt tgatccgcga gctgctcgct ccgcaggcgt ccgaacgcgc
      241 ttgctgcgaa gtcatcgacc acacactgga cggcgacgtt ctgtggaccg tggttcgcgt
      301 caccgccagg caggcgggcg tcatgggcct cgcggccggt gagtccgtct gctacatcgg
      361 ctgccatctg ctggaaagct cgggcggcga ttggggctat aagtccctcg atgagtccgt
      421 gcatccgtac tactactcgt gcccgctgcg ctatctcgac atggcgccgg tgcaaagctc
      481 cgagtggcgc gagcgggttc accgcttcca cgcggggcgc gctgtctagc ggcacgcatc
      541 ttccttcacc caaccggggc gagcactgcc ccgcgggctg tggttgctcc ttcatttcca
      601 agaggacatc accatgcctg caccttcttc cgcgaaaccg ctgtaccgca tcgacgaatg
      661 ccccgatctc atggccgacg gttgcgtcgg tgacgagcag ggcaatctcg tctttctctc
      721 gatctgggcg cgcgacaccg ccgttcagga gttcctcgcc cgcctgaccc tcggccggga
      781 tgaacaggga ctggatcagt tccacgtcat caccgagcag ggcgcatcca tcccggtctt
      841 cgtcggcaac gtcgagaacc tggaaaagcg catcacccgc gcctatcggc gaacgctgtt
      901 cggttcgctg acgaatgtgt ggctgttcga tcgtcgctgc gtgaagccag acaaggccaa
      961 cgccagcgcg ctggcgcttc tgcccaggga ttccgctcac cggctcgacc ggctgtggac
     1021 gctggtgcag gacacctgcc cgctgccact gctcgaccac tggcgcgaca ccgtgctgga
     1081 gctgttgcag acacggcgga tgctgaccgg tcttcccttg gccctcgggc cgctggaagg
     1141 ccatcggctg gccctcgatg tcccggcgct gacgaaggcg ctgggcgagc tgatccgcaa
     1201 cggcaccctc ggcgccacgc agtacgaact ggccgcgaac gcaccgcttc ggcgtgtggc
     1261 gtgagccatc cccacgggca tgcgcgccgc gcgtgcccgc cttccctcat ccatcaccag
     1321 gagaaatcca tggcactcat gtttccgcgc ctggcgcgca atttcatccg taacggctac
     1381 ttccccaccg acgagccgac gctggagcgg gccttgtccg cactggcgcc gtctcccggc
     1441 tccatgtcca tcctcgatcc ctgcgccggc gaaggcgtgg cgattgccga agccgcccac
     1501 gccctcgggc gcgagcaggt ccaggccttc gccgtcgagt acgacgccga gcgtgcccgc
     1561 cacgcacggc aactggtcga tcgctgcatc cacggtgacc tgatggacac gctgatctcg
     1621 cgccagtcct tcgggctgct gtggctgaac ccgccgtatg gcgacctgag caaggacgtg
     1681 aacggcaaca tcggctatca gggccagggc cgtgcgcggc tggaaaagct gttctatcag
     1741 cgcgcgctgc cgctgctgca gtacggtggc gtgctgatct tcatcgtgcc gtcctacgtg
     1801 ctcgacgccg agctggtcgg atggctgacg cgccacttcg ccgacctgcg catctaccgt
     1861 gcggtggaaa cgcagttcaa gcaggtggtg atcttcggtc gcaggattcg tcaacgcgac
     1921 caggcgtcgg agtcggtcaa agccgtgcgc ggtctgctgc tgcagatcgg acagggcgac
     1981 gccgaagccg aggagctgcc gctcgaatgg ccgttcctgc cgtacaccgt ccctgccagc
     2041 ccggccgagc cggagcactt ctatcgcgtg acgatggagc ccgagcagtt cgccgatgaa
     2101 gtcggccggc tgcaaggact ctggccggcg ctcgataccc acctgggtgc cgcgcagcaa
     2161 tcgctgcgtc cgcccgcgcg ggccttgtcg cactggcatc tcgccctggc cttggccgca
     2221 ggcgcgatct ccggcgtggt gacgtccaag agcgggcgcg tgctggtcgt caaaggtgat
     2281 acccacaagg agaagaccct ccagacggag tacaccgagc gcgacgacgg ctccgtggcc
     2341 gagacgcgca tcctcaccga caagttcgtg ccggtcattc gtgcatggga tttgacgctg
     2401 ggctcgccca cgtggggcga agtgctgacc atccgctgat cgctgttccc tgacggttcg
     2461 ccgtcgcttt catccaccca ccggggtcac gttgccccga tggggtgccg tggccccttc
     2521 ttccagaagg agataccacc atggcactcg cagtcctcaa cctcgcgtca caagcacgct
     2581 tttcgcccgg gcaggtggtc atgaccgccg gcgtcgacga gctggtccga cagggccggc
     2641 tcaaccccac gccgtacctg cgccgccatc ttcatggcga ttggggcgac ctggacgaca
     2701 gcgatcgacg gcagaacgat gccgcgctga agtccggcga ggatcgtctg ttctcgtcct
     2761 accaggtcac gcgcgacctg aagctctgga tcatcaccga atgggatcgc agcgtcacca
     2821 cgctgctgtt gcccagcgaa tactgatccg catccagcgg cctcgcgccg cttctttctt
     2881 cccacccagg ggcatgtcac cgccccgtgg gggaggtgca tgccccattg ctttggagca
     2941 tcaccatgtc cctcgatctc gaaaccgttc ccgaaaccgc tgtgcagggc gacctgctcg
     3001 aagcggccgc ttcccccctc acgctcagcc tgaccgactt cgtgtcggag ttcggcgacg
     3061 agctgctcga ttcgctcaac cgcgccaatc ctccggtcta caccggccag gtgcgggtgc
     3121 atcggcaact gatcctcgcc gcgctgaagc gcaagctgtt cccggcgcaa gccgatgtgg
     3181 tccacgccgt caccgagctg ttggtcgatc gcggcgaacg cgccgcgatc gtcaatggcg
     3241 agatgggctg cggcaagacg acggtgggta ttgccaccgc cgccgtgctc aacgccgaag
     3301 gctaccgccg caccctggtg ctctcgccac cccacctggt ctacaagtgg cggcgcgaaa
     3361 tccaggagac ggtggccggc gccaaggtct gggtgctcaa tggcccggac acgctggtca
     3421 agctgctgaa actgcgcgag cagttgggcg tgccggccca gggccaggag ttcttcgtcc
     3481 tgggccgcgt gcggatgcgg atggggttcc actggaaacc tgtcttcgtt cggcggcgca
     3541 cgcctcacgg cgacgtgggg gcctgcccgg attgcgggca tgtcatcacc gacctcgacg
     3601 gcgagccgat caacccggtc gaactcgaag ccgaggagtc ccgccgcaag tgcagccact
     3661 gccgtgcacc gctgtggtcg ttgatccgtc ccagaggcct gtccgccagc gaccagtcct
     3721 cgaccgtgct caaggcactg aagcgtattc caaccatcgg ggaagtcacc gcgcagaagc
     3781 tgatgcaaaa gttcggtgac gccttcctcg cgtcgatgct cggggacaac atccacgagt
     3841 tcatcaacct gatggatggc aacggcgagc tggtcttctc ggaccggcaa gcccatcgca
     3901 tggaacgtgc gatggccaac atggagttcg gcttcggcga gggcggctac cagccgtccg
     3961 agttcatcaa gaggcagctt ccccaaggca cgttcgacct gctcatcgcc gacgaggcac
     4021 acgagtacaa gaacggcggc tccgcccagg gccaggccat gggggtgttg gcggccaagg
     4081 cgcgcaagac gctgctgctc accggcacac tgatgggcgg ctacggcgac gacctgttcc
     4141 acctgctgtt ccgagccctg ccggggcgga tgatcgaaga cggctaccgc ccgaccacga
     4201 gcggcagcat gacct
//
LOCUS       DAOORV010000255         4207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_25027_length_4207_cov_13.930395, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000255 DAOORV010000000
VERSION     DAOORV010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25027_length_4207_cov_13.930395"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PKV56_18565"
     CDS             complement(<1..572)
                     /locus_tag="PKV56_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69854.1"
                     /translation="MATHSIPIPRQAWSVLIVSTIAFTVCFMVWMMFGVIGIPLKKTL
                     GLNATEFGLLTATPVLTGSLIRVPLGIWTDRYGGRIVMTLLMAATVPAIWLMSYATAY
                     WHFLVIGLFVGLAGGSFSVGTPYVARWFPKARQGMAMGVYGAGNSGSAVNKFAAPVLL
                     AIGGWTLVPQVYAAVMLGTVILFWLGSSSDP"
     gene            797..1309
                     /locus_tag="PKV56_18570"
     CDS             797..1309
                     /locus_tag="PKV56_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX69855.1"
                     /translation="MSPETARQVSALIDRIQTQFHEGHRRALPELLAMAATVEARGID
                     DGLVDELRAIGNALEQHMFKEEMRLFPMMEQGGNTLIGLLIEDLHREHIAHEHAMDAL
                     RAHLCTLTEAHGTDLALHQLIRGLDDLACQLALHIRAEDEELFPLFATPSSPGSPSTF
                     NPPSPATSTT"
     gene            1306..2319
                     /locus_tag="PKV56_18575"
     CDS             1306..2319
                     /locus_tag="PKV56_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HOX69856.1"
                     /translation="MNSTPPQSSASLPETDSASATTDPARTALLDALNELLEAERAGA
                     RVAMETGRSITDPELAALVEDIHKDEVRWCSMLMRTIKCLGATPSSATGAFHGKAMAI
                     ADLDERLKFLNRGQAWVTRKLQALIPQVDDARARADLTDMLQAHHQNISRVEDRFTAP
                     ATLEPQTEPQAEASPVPTEPGALIEFILTRFHEVHRQQLPELIQLAAKVEAVHADHAE
                     TPRGLTDLLTSMHSELLAHMEKEETILFPMLARGGSSFVTHPICVMMSEHEEHAHRLA
                     QLMALTHQATPPGDACRTWVQLCTATRQFAEDLEAHIQLENRVLFPQFDKSLVQERPE
                     TAR"
     gene            2316..2543
                     /locus_tag="PKV56_18580"
     CDS             2316..2543
                     /locus_tag="PKV56_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HOX69857.1"
                     /translation="MSKRFPMNPAHPERVCWGCDRYCSTDALVCGNGSVATLHPAELF
                     GEDWMNFGMDARTPPPTSLPDNASSEPAAKA"
     assembly_gap    2731..2830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2868..4052
                     /locus_tag="PKV56_18585"
     CDS             2868..4052
                     /locus_tag="PKV56_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HOX69858.1"
                     /translation="MKNALEGNRWLLWLGLQRQTVDARERGRDVLGAMLGLWVAGALT
                     WWGWGATSPWLAATMGASAVLLFAVPSSPLAQPWPVMVGNVMSALVGVAVFRWLGPTP
                     AAAALAAGLALALMFPLRCVHPPGGAVAITAVLGQAAVHDLGFGFALVPVAVNSALLL
                     VVALAWRRLTRPAPVHAPVVHGSRHSTADPAPQDRVGLTTADLDAALRDFNQLVDIDR
                     DALETLLRQAQMQAWQRRFGSVRCADIMSRDIVSVEFATPLNEAWALLHRHRIRALPV
                     VDRARRVIGVVTLVDFLKQARLDPQDHRSLGQRIRQLLAPTPGDYSDKAEVVGQIMSS
                     PVQTADEQESVVELVPRLSDQGLHHVPVVDGERRLSGMVTQSDLVAALYRSQFSDQPS
                     RS"
BASE COUNT          726 a         1357 c         1344 g          680 t
ORIGIN      
        1 gggtcgctgc tgctgcccaa ccagaacagg atgaccgtgc ccagcatcac cgcggcatac
       61 acctgcggca ccagcgtcca gccgccgatg gccagcagca cgggcgcggc gaacttgttg
      121 acggcagagc cggagttgcc ggcaccgtac acccccatgg ccatgccctg acgtgccttg
      181 gggaaccagc gcgccacata cggggtgcca accgagaacg agccgcccgc caaccccaca
      241 aacaggccga tgaccaggaa gtgccagtag gcggtggcgt agctcatcag ccagatggcg
      301 ggcacggtgg ccgccatcag cagtgtcatc acgatgcggc caccatatcg gtcggtccag
      361 atgcccaggg gcacgcgaat cagcgagccg gtgagcaccg gcgtggcagt caacagaccg
      421 aactccgtgg cgttcagtcc cagcgtcttc ttcagcggaa tgccgatgac gccgaacatc
      481 atccacacca tgaagcacac ggtgaaggcg atggtgctga cgatgagcac ggaccacgcc
      541 tggcgtggga tggggatgga gtgggtggcc atggagcgga ctcgtcttgg gtttgacgag
      601 actgtccgcc ggcaccgcct tcgtgaacat cagcacggtg gctacgcgca ccgcggcaga
      661 agaacgatgg cgcgcggcca ggacatagtt ctgaagaact acgccggggc cctttggttc
      721 agaacttgac ctgggtcaac gtcaacggct tgcgccctcg cccagactga accggaatag
      781 gctgcctcca ccgaccatgt cccctgaaac cgcccggcag gtcagtgccc tgatcgaccg
      841 catccaaacg cagttccatg aaggccaccg ccgggcactg cccgagctac tggccatggc
      901 cgcgacggtt gaggcgcgcg gcatcgacga cggcctcgtt gatgaactgc gcgcgatcgg
      961 caacgcgctc gaacagcaca tgttcaagga ggagatgcgg ctgtttccga tgatggagca
     1021 aggcggcaac acgttgatcg ggctgctgat cgaggacttg catcgcgaac atatcgcaca
     1081 tgagcacgcc atggacgctc tccgcgcgca cctttgcacg ctgaccgaag ctcacggcac
     1141 agacctggcg ctccatcagc tgattcgagg cctcgacgac ctggcctgtc agctggcact
     1201 gcacatccgg gcagaagacg aggaactgtt tccgctcttc gcaacgcctt catctccggg
     1261 ctccccttcg acattcaacc caccgtcgcc cgcgacctct accacatgaa ttcgacccca
     1321 ccgcagtcat ccgcctccct gccggaaacg gactccgcat cagccaccac ggacccggcc
     1381 cgcacggcct tgctggacgc gctcaacgaa ctgttggagg ccgagcgtgc cggtgcccgc
     1441 gttgcgatgg agacgggacg gtcgatcact gaccccgagc tcgcggcact ggtggaagat
     1501 atccacaagg acgaggtccg ctggtgcagc atgctcatgc gcacgatcaa atgcttgggg
     1561 gccacgccct ccagcgcgac cggtgcgttc catggcaagg cgatggccat cgccgatctg
     1621 gacgagcgat tgaagtttct gaaccgaggc caagcctggg tgacccgcaa gctccaggcc
     1681 ctgattccgc aggtcgacga cgcacgggca cgcgccgact tgacggacat gctgcaggcc
     1741 catcaccaga acatcagccg ggtggaggac cgattcacag cgcccgccac tttggagccc
     1801 cagacggaac cgcaggccga agcgtcaccc gtgcccaccg agcccggtgc gctgatcgag
     1861 ttcatcctga cgcgcttcca cgaggtccat cgtcagcagc ttccagagtt gattcagctc
     1921 gcggccaagg tcgaggccgt gcacgcggac cacgccgaga cgccgcgggg cttgaccgat
     1981 ctgctgacgt cgatgcacag cgagttgctg gcgcacatgg agaaggagga gacgatcctc
     2041 tttccgatgc tggcgcgggg cggcagttcg ttcgtgaccc acccgatctg cgtcatgatg
     2101 tccgagcatg aggaacatgc tcacagactt gcgcagttga tggccttgac tcatcaagcg
     2161 acgccgcccg gcgatgcctg ccgcacctgg gtccagcttt gcacggcgac gaggcagttc
     2221 gccgaggact tggaggcgca catccagctg gagaacaggg tgctgttccc gcaattcgac
     2281 aagtcgctgg tccaagagcg gccggagact gcgcggtgag caagcggttt ccgatgaatc
     2341 ctgcccaccc cgagcgcgtc tgctggggct gcgaccggta ttgctcgacg gacgccttgg
     2401 tttgcggtaa cggcagcgtg gcgacgctgc atcccgccga gctctttggt gaggactgga
     2461 tgaacttcgg catggacgca cggactccac cacccacgag cttgcctgac aacgcttctt
     2521 ccgagccagc ggcaaaggct tgagctgtgt ccctccagcc attcgatctc gggcaaggtc
     2581 tccgaaagtc cggggcatga cgctggacgc atgcgcgctt tcctccagcg gccggcaacg
     2641 tcggctgttt cacggttgcc atcgaggaac ggccttcgaa attgaggtca cacgcatcga
     2701 cacgagtcct ggaagtcacc tcgtgatcgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn ggtcatgcgc cgcacaggct caaggaaccg acaaacgatg aagaacgctc
     2881 tggaggggaa ccgttggctg ctgtggctgg ggctgcagcg ccagaccgtg gatgcgcgcg
     2941 agcgcggccg cgatgtgctg ggcgccatgc tcggcctgtg ggtggctggc gcgctcacgt
     3001 ggtgggggtg gggcgcgacc tcgccgtggc tggccgcgac gatgggcgcg tcggcggtgc
     3061 tgctgttcgc ggtcccgagc agtcctctgg cccagccctg gccggtgatg gtgggcaacg
     3121 tgatgtcagc cctcgtgggc gtcgcggtct tccggtggct ggggcccacg cccgcagccg
     3181 ccgctttggc agcaggcttg gcgctggccc tgatgtttcc tttgcgctgc gtccacccgc
     3241 ccggcggcgc cgtggccatc acggccgtgc tgggccaggc cgcggtgcat gacctggggt
     3301 tcggtttcgc actggtgcct gtggcggtca actcggcctt gctgctggtc gtggcgctgg
     3361 cctggcggcg gctgacacgg cctgcgcctg tgcacgcgcc ggtggtccac ggcagccggc
     3421 acagcactgc ggatcccgcg ccacaggatc gcgtgggtct gaccaccgcg gaccttgacg
     3481 ccgcgctgcg cgacttcaac cagttggtcg acattgatcg ggatgcgctc gaaaccctgc
     3541 tgcgccaggc ccagatgcag gcgtggcagc ggcgcttcgg cagcgtgcgc tgcgcggaca
     3601 tcatgtcgcg cgacatcgtg tcggtggaat tcgccacgcc actcaacgag gcctgggcct
     3661 tgctgcatcg gcaccgcatc cgcgccctgc cggtggtgga ccgggcccgc agagtgatcg
     3721 gcgtggtgac gctggtcgac ttcctcaagc aggccaggct cgacccgcag gatcaccgca
     3781 gcctggggca acgaatccgg caactcttgg ccccgacgcc gggcgactac tcggacaagg
     3841 cggaagtggt gggccagatc atgagcagcc cggtgcagac tgccgacgaa caggagtccg
     3901 tcgtcgaatt ggtgcctcgc ctgtcggacc agggcctgca ccacgtgccg gtggtagatg
     3961 gcgaacgccg cctgtcgggc atggtcacgc agtccgattt ggtggcggcg ctgtaccggt
     4021 cgcagttctc cgatcagccc agccgcagtt gacggctgcg ctcccagcgg ctccgttctc
     4081 ggtcagcgcc aacacctcag tatcgctgcc acgacccatg gactgcgcgc agccggtgaa
     4141 gagagattcg ctttggcgat gcaccaagca gtcgccgtcg aaggttccgg gtatcgccgc
     4201 ccttcat
//
LOCUS       DAOORV010000256         4192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_25150_length_4192_cov_5.795746, whole genome shotgun sequence.
ACCESSION   DAOORV010000256 DAOORV010000000
VERSION     DAOORV010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25150_length_4192_cov_5.795746"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PKV56_18590"
     CDS             <1..1543
                     /locus_tag="PKV56_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69859.1"
                     /translation="ASAIDRQAVEWMEAYLADSAGETRMRLVVSIQPTCRTTEADLLE
                     LVRLAERYAGRAAFRVYPERSLRDRSSNLLCFSARDASAVVVLGPAENLGLGPTSPSQ
                     ANIVTSVSAPTLEACRKWFDYLWGVAGPLQPEIAASMPQLVIPEGDAEAARLWREYRT
                     RCFAQPQGGAAQPKVSVDVKTGEVTLTDHNDRPVESPTAELGVPKLDGVADGIARVFE
                     QGLLVAVDKTSRVPPLEAPVKPEWFGVDSFKQTGMVSSKTSFKVAPFDEATLKKVDRL
                     RRASGDILPHYTYALADGMRWIPKAAIPLFEAALTEANDAAKKLLGVTVGDDIDAFLS
                     TQRDRIRADAQRMYEAYHPGGKIPETAVGNILDELKARLGKTRGEALIPKVAYSPVAF
                     SVGKDTAWSSPWGQAFQLLKGVAEFPREAMTSRFFWQGVRADEDELIQAMNVAGDHLV
                     VEYGSRKAKQRAEHELAIIKQLESSQADVRERCAALWALVTAGDDSVACELLEAAART
                     GAASA"
     gene            1599..3404
                     /locus_tag="PKV56_18595"
     CDS             1599..3404
                     /locus_tag="PKV56_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69860.1"
                     /translation="MGLNQSETIGKDLPPGAKLWLNVSRFDERQRRLRLVLPKPATCW
                     DYAALIDLVVHESGVDGYRFLGQTVADRVALLRRHKDPAASHFMAWFERAIAPHMAVL
                     DRRRRVPDNHYCVDVAPLPLVSSIPATVRELGPNRASAAQWSATLHGLTEKGVRREEL
                     QLSGVIARVDQLPSDAKLTKAQVLRLINLSHAMPKLVSESRFGFAARAGWRECCDRLP
                     AKEHKRRRLHGIGHGMAHVRFRHRSLGWAVARTRFSDLLVEQRDWWIVLDESGRLVEA
                     ANGGFVSPEDALEFAELQIGRRFSRWGKDQAAARWEHFSLPGGDDYRELLLQLDDWPE
                     TYRPRHYRTRNALVHVRTSLRRTTAGRRVLFLDEVQSDWHADLHALAKRAPTAAAEAR
                     IATAPFGKEWPLLTMKVMLWWAQRCGAEGLAWSSAELQQQRWHGYGPPDLLYRKMLPE
                     AGLALSKALTLRLGAASLRIRGGDRVVERGAHGWEVRGRDGQKLTKAFRNRGQAEAFA
                     DLTGEFIVLDMPVLWLDGAERLASIPLFGSGSIDTWAAGVQTMTMNTTRRNGKSGGEQ
                     ASGDADLHGTRRLPRRGPTASPSDASNFNHCPHHG"
     gene            3397..3702
                     /locus_tag="PKV56_18600"
     CDS             3397..3702
                     /locus_tag="PKV56_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69861.1"
                     /translation="MADSPASEDDVLAIATQNERVCPMPQAWNRLYDLLPGKSRAGAG
                     WEPSLPLILSAWHDSTAMAKGVRFREHIEWAASHGALEEVRQFLATLPEDQWFHLSD"
     gene            complement(3753..>4192)
                     /locus_tag="PKV56_18605"
     CDS             complement(3753..>4192)
                     /locus_tag="PKV56_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69862.1"
                     /translation="RQPGPQRQAGTIWGPTSFLDVGTNATGDDKGDRSPLALFDTGPV
                     GTGAGSTPDGMSWPTRPPPATAHTFGTNADFANQPWWTPEQPQSQANISDSPAPTLGG
                     ATPLEGATALALNTETCRRMPEAPQLDPDFGSALHALSPWRRD"
BASE COUNT          699 a         1316 c         1459 g          718 t
ORIGIN      
        1 tgcaagcgcc atcgatcgac aggccgtcga gtggatggaa gcctacttgg ctgacagcgc
       61 gggcgagaca cgaatgcgcc tggtggtcag cattcagccg acctgccgaa cgaccgaggc
      121 ggacttgctg gagctggtgc gcctggcaga gcgctacgcc gggcgcgcgg cgttccgtgt
      181 ctacccggaa cggtcgttgc gcgatcggtc ttcgaatctg ctgtgcttct cggcacgcga
      241 cgccagcgca gtggtcgtcc tcgggccagc ggagaacctg ggccttggtc cgacctcgcc
      301 ttcgcaggcc aacatcgtca cctccgtgtc ggcacccacc ctggaggcct gccgcaagtg
      361 gttcgactac ctctggggcg tcgcagggcc gctgcagccg gagatcgcgg cttcgatgcc
      421 gcaactggtc attccggagg gcgatgcgga agcggcacgg ctttggcgag agtaccggac
      481 gcgttgcttc gcgcagccgc agggcggcgc cgcgcagccc aaggtgtcgg ttgatgtgaa
      541 gaccggcgaa gtcacgctga ccgatcacaa cgatcggccg gtggagtcgc cgacggcgga
      601 actcggtgtg cccaagctcg acggcgtggc cgacggcatc gcgcgtgtct tcgagcaagg
      661 cctgctcgtg gctgtcgaca agacgagccg tgtgccgccg ctcgaggcac ctgtgaagcc
      721 ggagtggttc ggtgtcgaca gcttcaagca gaccggcatg gtcagctcca agacttcgtt
      781 caaggtggcg cccttcgacg aagccacgct gaagaaggtc gatcgcctca ggcgggcatc
      841 cggtgacatc ctgccgcact acacctatgc gcttgcggac ggcatgcgct ggattccgaa
      901 ggcggccatc ccactattcg aggccgcttt gaccgaggcc aatgacgccg ccaagaagct
      961 gctcggcgtc acggttggcg atgacatcga cgcgttcctg tcgacccagc gggaccgcat
     1021 acgcgccgat gcgcaacgga tgtacgaggc ctaccatccg ggcggcaaga tcccggagac
     1081 cgctgttggg aacattctgg atgagctcaa ggcgcggctg ggcaagaccc gcggtgaggc
     1141 gctcataccc aaggtggcct actcgcctgt ggccttcagt gtcggtaagg acacggcgtg
     1201 gtcatcgcct tggggacagg ccttccagct gctcaagggg gttgcggagt ttccgcggga
     1261 ggcgatgacg agccgcttct tctggcaggg tgtgcgtgcc gacgaggacg agctcatcca
     1321 ggcgatgaac gtcgcaggcg accacctcgt ggtggagtac ggaagccgca aggcaaagca
     1381 gcgtgctgag catgagctgg cgatcatcaa acaactcgag tcgagtcagg ccgacgtgcg
     1441 cgagcgttgc gccgcgctgt gggctctggt gacggccggt gacgactcgg ttgcctgcga
     1501 gcttctcgag gccgccgcgc ggacgggtgc cgcgagtgcg tgaccagggc ccgggccact
     1561 ttacccaaca ggcgtcctga accacctgta acagcgccat gggcttgaac caatccgaga
     1621 cgatcggcaa ggacctgccc ccgggggcga agctctggct gaacgtcagc cggttcgacg
     1681 agcgccagcg ccgtctgcga ctggtcctgc ccaagccggc aacctgctgg gactacgctg
     1741 cgctgatcga tcttgtcgtg cacgaaagcg gagtcgacgg ctaccggttc ctcggacaga
     1801 ccgtggcaga ccgcgtggcg ctactgcggc gccacaagga tcccgcggcc tcgcacttca
     1861 tggcctggtt cgagcgggcc atcgcgccgc atatggcggt gctcgatcgc cgccggcgcg
     1921 tcccggacaa ccactactgc gtcgacgtgg cccccctacc gctggtctcg tcgattccag
     1981 cgaccgtgag ggaactggga ccgaaccgtg caagtgccgc gcagtggtcg gcaactcttc
     2041 atggcctgac cgagaagggc gtcaggcgcg aggagttgca gctatcgggc gtgattgcgc
     2101 gcgtggatca gttgccgagc gatgccaagc tcaccaaggc gcaagtgcta cgcctgatca
     2161 acctgtccca tgcgatgccg aagctcgtca gcgagtcccg cttcgggttc gcggctcggg
     2221 ccggctggcg tgagtgctgc gaccgactgc ctgcgaagga gcacaagcgt cgccggttgc
     2281 atggaatcgg ccacggcatg gcccatgtgc gctttcggca ccgctccctg ggctgggctg
     2341 tggcacgaac ccggttcagt gatctgctgg tggaacagcg ggactggtgg atcgtcctcg
     2401 acgagtcggg gcgtcttgtc gaggccgcca atgggggctt tgtctcgccg gaggacgcgc
     2461 tcgagttcgc ggagctccag atcggccgac ggttctcacg ttggggcaag gaccaggcag
     2521 cagcgcgttg ggagcatttc tcgttgccgg gcggcgacga ctatcgtgag ctcttgcttc
     2581 agctcgacga ctggccggag acctatcggc cacgccacta ccgaacgcgc aatgccttgg
     2641 tgcacgtgcg tacgagcctg cgccggacca ccgctgggcg ccgggtcctg ttcctggatg
     2701 aggtgcagag cgactggcat gcagacctgc atgcgcttgc caagcgtgcc cctaccgcgg
     2761 cggcagaagc acgaatcgcg actgctccgt tcggcaagga atggccgctg ctgacgatga
     2821 aggtcatgct gtggtgggct cagcggtgtg gcgccgaggg gctcgcatgg agcagcgcgg
     2881 agcttcagca gcagcgctgg cacggctacg gcccccctga cctgctgtat cgcaagatgc
     2941 tgcccgaggc gggtctcgcg ctatccaagg cactgacgct tcggctcggc gcggcgagcc
     3001 ttcggattcg tggcggcgac agggtggtag aacgcggcgc gcatggctgg gaggtccgcg
     3061 gccgggacgg gcagaagttg acgaaggcgt ttcggaatcg tggtcaggca gaggcgttcg
     3121 ccgacctgac gggcgagttc atcgtgctgg acatgccggt gctgtggctc gatggggcgg
     3181 agaggttggc cagcatcccg ctgttcggct ccggaagcat cgacacttgg gccgctggcg
     3241 tgcaaacgat gacgatgaat acgactcgac ggaacggcaa gtcaggcggc gagcaggcga
     3301 gcggtgatgc cgacttgcat ggcaccagac ggttgccgcg caggggcccg accgcgtctc
     3361 caagcgatgc ctcgaacttc aaccactgtc cgcaccatgg ctgattcccc ggcttccgaa
     3421 gacgacgttc tcgcgatcgc gacacaaaac gagcgggtct gtccgatgcc gcaggcgtgg
     3481 aaccgtctgt acgacctgct gcccggcaag agccgggcgg gggctggctg ggagccatca
     3541 ctcccgctga ttctctcggc gtggcacgac agcacggcga tggcaaaggg cgtgcggttt
     3601 cgggagcaca tcgagtgggc cgcaagtcac ggcgccctcg aggaggtgcg ccagttcctc
     3661 gcgacacttc ccgaggatca gtggttccat ctcagtgact gacccttctc agtgcgcctc
     3721 gcgttcactt tggcggcaca catccagcaa ggtcagtccc ggcgccaggg actcaatgcg
     3781 tgcagtgcac tcccgaagtc cgggtcgagt tgcggcgctt cgggcatgcg ccgacaagtc
     3841 tcggtgttca acgcaagtgc agtagcgcct tccagtggtg tcgcaccacc caaggtcggc
     3901 gcgggcgagt ccgagatgtt ggcttgactc tggggttgtt ccggcgtcca ccagggctgg
     3961 ttcgcgaagt cggcgttggt cccgaaagtg tgcgctgtcg ctggtggcgg ccgcgtcggc
     4021 catgacatcc cgtccggggt cgagccggca ccggtaccca cgggtcccgt atcgaacagc
     4081 gcgagcggcg accggtcgcc cttgtcatca cccgtcgcat tcgtgcccac atccagaaag
     4141 gaagtcggtc cccagatcgt gccggcttgg cgctgcggac caggttgtcg ct
//
LOCUS       DAOORV010000257         4156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_25434_length_4156_cov_11.028530, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000257 DAOORV010000000
VERSION     DAOORV010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_4156_cov_11.028530"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKV56_18610"
     CDS             <1..245
                     /locus_tag="PKV56_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69863.1"
                     /translation="ERAGQLLGVHWTTVYRLRARFLADPRTSALVPDAGGRRGEPWRL
                     APPVEAIVSSVVDQWVPRQRELAHPVAPTIVDMCCA"
     gene            674..1696
                     /locus_tag="PKV56_18615"
     CDS             674..1696
                     /locus_tag="PKV56_18615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX69864.1"
                     /translation="MYPQSKEESSEMLRLALPLLARHGSGCQPPSYTVWYEYVQGAND
                     ALRRVLDARIANPDPLTPDETTALFRKYVVDRELALTDELQSRLLKALGALSTSADSS
                     DVDAARFGQALEHGNHRLSTEPDVEGLKELIESLVAETRRMQVSNADLRRRLDESKRD
                     LLDITSKVTVLRSEVLLDPLTRLLNRRGLEKAMDDMLMSDTGGLTGAALLMVDIDHFK
                     RINDTHGHVLGDRVLRAVAQVIKGSIGPDGVACRYGGEEFAILMPATSLGHAQATAND
                     FRLKISEGRIRRVDGQTVGGISVSVGIAMFDRGQSMDSWISRADEALYASKRSGRNRV
                     TVSGDI"
     gene            complement(1957..2160)
                     /locus_tag="PKV56_18620"
                     /pseudo
     CDS             complement(1957..2160)
                     /locus_tag="PKV56_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(3076..3570)
                     /locus_tag="PKV56_18625"
                     /pseudo
     CDS             complement(3076..3570)
                     /locus_tag="PKV56_18625"
                     /inference="COORDINATES: protein motif:HMM:NF025079.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            3593..3811
                     /locus_tag="PKV56_18630"
                     /pseudo
     CDS             3593..3811
                     /locus_tag="PKV56_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3896..4147
                     /locus_tag="PKV56_18635"
     CDS             3896..4147
                     /locus_tag="PKV56_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOX69865.1"
                     /translation="MSRQTMSFALPESMREYIDNRVAAGNYGNTSEYIRDLVRRDQEE
                     QAKKRLRDLIEEGLASGPGRRRTKADEKELLAIARGEID"
BASE COUNT          743 a         1289 c         1400 g          724 t
ORIGIN      
        1 ccgagcgcgc gggccaactg ctgggtgtcc actggaccac ggtctatcgc cttcgtgctc
       61 gatttctggc ggatccgaga acgtctgcgc tggtaccgga cgcgggaggc cgccgcggcg
      121 aaccctggcg gctggcgcct ccggtcgagg cgatcgtctc gtcggtcgtc gaccagtggg
      181 tcccgcggca gcgcgagctc gcacaccccg tggctcctac aattgttgat atgtgttgtg
      241 cctgatgaac gacttgatgc ggatcaatag cgcgagttgc tggcccggtc gtcttccgat
      301 ccgaggtcga caagcttcgg gaggagccgg tgcccgacat gaccgcccat ctcatcggta
      361 cccgcaaatc cgcttttggc aggaccttga agtggatgat ccaaggcatg cggtcgttct
      421 ggacgcaagc gtggtgcgcc cgccgcaaca ggtcgactgc aatcgatcgc tgaccgcgcg
      481 ggcaggactc gcggtggctg cggcccttga ggcaatcggc gctgtcggct cgtggacagc
      541 gggcttgttc ggtcgctggc ccaaaggcgg cgggcaggac cgaatgttgg gtagtgagat
      601 ccccacctat tccttgcgag aacgaggatc gaagtcgacc cgatagtgtt ggcggattgt
      661 cgagctgaaa gaaatgtacc cgcagtccaa agaagagagt agcgagatgc tccgcctggc
      721 gctccccttg ctggcgcgtc atggctccgg atgccagcca ccgagctaca cggtctggta
      781 cgaatacgtt cagggggcca acgatgcgct gcgccgagtg ctcgatgccc gaatcgcgaa
      841 ccctgacccg ctcacccctg acgaaacaac tgccctgttc aggaagtacg tcgtcgaccg
      901 cgaacttgcg ctgaccgatg aacttcaatc gcgacttctc aaggcattgg gggctctgag
      961 cacctccgcc gactcgtccg atgtagatgc tgcgcgcttt gggcaggcgc tggagcacgg
     1021 caaccaccgg ttgtcgactg aacccgacgt tgaaggtctc aaggaactca ttgagtcgct
     1081 tgtcgccgag acgcggcgca tgcaggtgtc caacgccgac ctgcgccgcc gcttggacga
     1141 gagtaaacgc gacttgctgg acatcacctc caaggtaacc gtgcttcgca gcgaggtgct
     1201 gctcgacccc ttgacacggt tgctgaaccg ccgcgggctg gagaaagcga tggacgacat
     1261 gcttatgtcg gacaccggcg gactgaccgg ggccgccctg ctcatggttg acattgatca
     1321 cttcaagcgt atcaatgaca cgcacggcca tgtactcggt gaccgggtgc tccgggcagt
     1381 tgcccaggtt atcaagggca gcatcggtcc ggacggggtg gcctgtcgct atggcgggga
     1441 agagtttgcg atcctgatgc ctgctacgtc gttgggacac gcgcaggcca cggccaatga
     1501 ttttcgattg aagatctccg aaggccgaat ccggcgggtc gacgggcaga cggtcggcgg
     1561 catcagcgtc tctgtcggca ttgcgatgtt cgaccgcggg cagtcaatgg actcgtggat
     1621 cagtcgcgcc gatgaggcgc tctatgcatc aaagcggagt ggtcgcaacc gggtcacggt
     1681 ttccggcgac atctagctgg cgcagccgca gacccctcta cggctgaagc gagcccggtg
     1741 cgcggtcgaa ggctttagcg aggtcgcgca cgcaatggcc gattggaccc aaccgcgcgc
     1801 cttcgagcct cccgcctagg cgtcgtgttc caagaggttg ctcgttgctc acagccagct
     1861 cgctttggtt gaaccagcca ggcccaaaga aggatgctca gggctgccca gagaaacaca
     1921 attgaaagag cgccgatccg gctggctgcc ggcaatgacc gtgtcgatcg cggcgtcgag
     1981 catcgtgttg acgagctcgg cgtctggtcg cgtgccgatc gaccagctga ccaccatccc
     2041 gtcgaagcaa tcgatcatcg gcgacaggta gaccttgccg gccgggatct ggaactccgt
     2101 gatgtcggtg agccacttcc tgttgggcgc accggcagtg aagtcccggt tcaggaggtt
     2161 caacgagtgg tccagccaga tgctgccgcg acgcaagctc ttgcgcagcg acatcatcgt
     2221 gcacgcctcg taggcccgga atcccgagcg gggatcgagg tcacgcacca ggccatgcca
     2281 ggctgcaccc acgtccggga tgggagcgtc ttccttgatc tcggtcgccc catggcgctg
     2341 cagttctgcc cacgccttcc actgctcgaa tcccggatcc ccttcccgcc caccgaagtc
     2401 cagatccttc agtgccgcga ggcaggcgtg gacgcgctgg ctgtcttcgg ccagggcctt
     2461 gcgcacctgc gaaacgaagc tgccgcctgc ggtctcggca agtccagcga ggagctccct
     2521 ggcctcgatg acgcgggcct gccaggtctt ggactcgtcg tgcagaacag ctcgcgcctt
     2581 ggcagcttgt tggacgaggc tggtcgtgcc ccgcacacgg ttcgactggg ccttgtccgc
     2641 tgcctcgcgg aacagttgct ggctgcgccg gctggatagc agcagcgcca cgtcggtcag
     2701 ctccaacagg gttacccgca ggaagcagac caactcgatc agctggcgtg acggcttgat
     2761 ctctctcgtc ttcacggggc gccgggcctg gacttgtcgg gcgtaggcct gctgcttggc
     2821 cagcgacacg gcacccaggt tccagtcgtg ggcgccaagc tctttcaagt agcggacctt
     2881 gtcgagggtc tcggcaaggg tgctggggcc gtggcgcttc gacggcgtct tcagccactc
     2941 caggtgcgtg ccgtctccat tcggtcgggc gctgtaggca gagtcgatca ccttgctggc
     3001 ggcggaaggc ggcacggctg cggtgacctc gctgaggatc tgggcctcaa ccgccgcata
     3061 tgctgctctg gcccagtcct gcagtcgacg cgcgccgggg atgaggatgc gacgcgtgaa
     3121 cagccactgg caggccgatt gaacgaggtc gtcggagtgg gaggcgtccg cggcctgagc
     3181 caacagcgtg gctgtcagcg cggcttcgtc gtcaggcgca agttcgcgca ggccgaggta
     3241 ggtcttggcc cagagctggt gcttcgaaag cgtctgccgg cgcttgtaga tcgagcgcag
     3301 gctggcaata gacggcggag agaccgccag cgcctcggca gtcttgcgca ggaggccgcg
     3361 aggcagtacg ttgaagccgt ccagtggtcg gccggccacg cgcatgaaaa gcagcatcag
     3421 cgccgccggc aggcgatggt cgctgcggaa ctgcgcctga atggccgcca cgtcgtccga
     3481 gagcagcggg aagaactgct ccaggtcgaa atcgctcagg cgcggcggca aggcctcctg
     3541 gccgacgaag cgaagggcga acgcgggcat ggcatcgggc agcgaaaggt ggacggccat
     3601 cgtgctggcc tggaacaccg cccggatgca tgaggtggtc gagcggctca ggcgcgacaa
     3661 gatcgaagtg gaagacgact ggcttcgccg catcgggccg gcgcacttct cgcacatcaa
     3721 cttccgcggc acgatgagat tcggtgtcga gaagttcgct gcggcgctga ttcagcgggc
     3781 gccgagccag gcgactcggg cggcatcgtg agcggcgtca gcgatcgcag gcccgcagga
     3841 cggagagtcg ccttggagtg ttgacaaccg ttgtcaaaca gcccaagatt cccgcatgag
     3901 ccgccagacc atgagcttcg ccctgcccga gtcgatgcgg gagtacatcg acaaccgggt
     3961 cgccgcaggc aactacggca acacgagcga gtacatccgc gacctggtgc ggcgtgacca
     4021 ggaagagcag gccaagaagc ggctgcgcga tctcatcgaa gagggcctgg cctccgggcc
     4081 cggtcgacgt cgcaccaagg ccgacgagaa ggaactgctg gcgatcgctc gcggcgagat
     4141 cgattgaagc cggcgg
//
LOCUS       DAOORV010000258         4150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_25495_length_4150_cov_8.076923, whole genome shotgun sequence.
ACCESSION   DAOORV010000258 DAOORV010000000
VERSION     DAOORV010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25495_length_4150_cov_8.076923"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1109)
                     /locus_tag="PKV56_18640"
     CDS             complement(105..1109)
                     /locus_tag="PKV56_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69866.1"
                     /translation="MKPVNAFPGLLHAFFYERLVQQLNVSPHTVRSYRDTWRLFLRFA
                     AERHKRPVAELTLTDLTATEVAAFLQHSEQARKVSIGTRNCRLAALRSFYGFVAEREP
                     TAIAQCAAVRHIPTKKAPKPAAQYLEPNEIEAILAQPDRATLEGQRDHALLSFLYNTG
                     ARIQEALDVCPEAIRFESPMSVRLFGKGRKERICPLWPETVALLKALLRRQPRTATEP
                     LFVNRYGGPLGASGARFKLAQYVRAAAVITPSLSSKGVSPHRFRHATAVHLVAAGVDV
                     TVIRSWLGHVHLDTTNHYAQANLETKRQALERLDPSSKIAKPPRWRRDASVLAWLDSL
                     "
     gene            complement(1106..2470)
                     /locus_tag="PKV56_18645"
     CDS             complement(1106..2470)
                     /locus_tag="PKV56_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69867.1"
                     /translation="MNAWPDVNGDAIGRYLRQLRLRTSHTARIYRGILAGFQRLAEQR
                     GDGTVDLTIIVEWLRERARHCNEDLVAHQSRVLDRFLDFLVRDGLIAVNPLADLRAQY
                     GLRGTPPIVRALLCTDPHKALEAVRCLPPFASFLGDFLRGHAELMRALGRQYGTQTAR
                     FLRFDRFLQGRPDLAGSSPTELLRQWAGEHPTTSHAYQCQLLRNDLARAWRRLDPDLP
                     MPRPDPRLWRRVERRQPHLFTPAEVRLLLDTALHWPSPRAALRPLSLYTMLVLAYCAG
                     LRLGEIVHLDLGDVDLTRGEIAIRNTKFFKSRTLPLAPSVVEALRAFVGARHSAGGPQ
                     IESSGLFWHEQHAGRYSDTMAEKLLVKVMRSAGLKPSSGRVGPRIHDLRHSFVADRVL
                     TWYREGINPQQRLPYLATYLGHKDIHSTLVYLTVTQELLQQASERFRDFGAHALHADA
                     EVLS"
     gene            complement(2467..3486)
                     /locus_tag="PKV56_18650"
     CDS             complement(2467..3486)
                     /locus_tag="PKV56_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX69868.1"
                     /translation="MTRWRSSHTAGIHQLLRVVRGSWPVAPAARSPSELYFDGLLAEF
                     AQWLHAQRGLAARSIGVMVAEARRLLSWYGGSADAFDLRAMRLADIDAYVQWRCPALR
                     RVSRRNVTQQLRCFLKFLHVTGRTALDLAARVISPTLYACEAMPSLLDPEQIRAVLAS
                     TGQDHSSKGLRDYAILVLLATYGLRAGEIVQLRLDDVDWRGDRFVVRHTKTGLQSDLP
                     LVPAVGDALLAYLRRGRPVTDAREIFVHTKAPYRSFSRGSSLYLLVRLRLEGAAVSPA
                     GKCGPHTFRHARAVSLLRASATTKTIGDLLGHRSAESTRPYLKLATDDLRDVALEIPG
                     QEVQA"
     gene            complement(3978..4150)
                     /locus_tag="PKV56_18655"
                     /pseudo
     CDS             complement(3978..4150)
                     /locus_tag="PKV56_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          786 a         1295 c         1401 g          668 t
ORIGIN      
        1 cggccgtaat cagcaggact ccgtaatgtg aaggacgggc gccgggtgcc tcggagcggc
       61 gccattcgtg tcggtttttt ccttcacatt acttcgcccc gaggtcagag cgagtcaagc
      121 caagccaaca cgctcgcatc acgccgccaa cgaggcggct tcgcgatctt cgaggacgga
      181 tcgagccgct cgagcgcttg tcgcttggtt tccagattgg cctgggcata gtggttggtg
      241 gtgtccaggt gaacatgccc gagccagctc cgaatcaccg tcacgtcgac gccggcagcg
      301 accaagtgga cggccgtcgc atgccggaat cggtgtggcg aaacgccctt cgacgacagc
      361 gacggagtga ttactgccgc agccctgaca tattgtgcga gcttgaagcg cgcgccggag
      421 gcaccaagcg ggcctccata gcgattcacg aacaggggct cagtcgcagt gcgaggctgt
      481 cgtctgagca gggccttgag cagcgcgacc gtctcgggcc acaacgggca gatgcgctcc
      541 ttgcggcctt tgccaaagag gcgaacagac atgggcgact cgaatcggat cgcctccgga
      601 cagacatcga gtgcttcctg tatccgcgcg ccggtgttgt agaggaagga cagcagtgca
      661 tggtcgcgct gcccttccaa ggttgcgcga tccggctgcg ccaggatggc ctcgatctcg
      721 ttgggttcga ggtactgcgc tgccggcttc ggcgccttct tggtcgggat atgccgtacc
      781 gcggcacact gggcgatcgc cgttggctcc cgctcggcaa cgaagccgta gaagctgcgc
      841 agcgcggcga gccgacagtt gcgcgtaccg atcgagacct tgcgcgcctg ttcgctgtgc
      901 tgcaggaagg ccgccacctc ggtcgctgtg aggtccgtca gggtcagttc ggcgacgggt
      961 cgcttgtgtc gctcggcggc aaaccggaga aacagccgcc acgtgtcacg gtaggaccgc
     1021 accgtgtgcg gcgagacgtt gagctgctgg accagccgct cgtagaagaa ggcgtgcagc
     1081 agtccgggga aggcattgac cggcttcatg acagcacctc cgcgtcggcg tgcaacgcgt
     1141 gcgcgccgaa atctctgaag cgctcgctcg cctgctgcag caactcctgc gtgaccgtca
     1201 ggtacacgag cgtcgaatgg atgtccttgt ggccgaggta ggtcgccaga tacggaagcc
     1261 gttgttgcgg attgatgccc tcgcggtacc acgtgagcac gcgatcggcc acgaacgagt
     1321 ggcgaagatc atgaatgcgc gggccgacac gcccactgct gggcttcagc cccgcacttc
     1381 gcatgacctt cacgagcagc ttctcggcca tcgtgtcgga atagcgcccg gcatgctgct
     1441 catgccagaa gaggcccgat gactctatct gcggccctcc cgccgagtgc ctggcgccga
     1501 caaaagcgcg cagcgcctcc accacgcttg gcgccagcgg caaggtgcgg gacttgaaga
     1561 acttcgtgtt gcgaatggca atctcgccgc gggtcagatc gacgtcgccc aggtccaggt
     1621 gcacgatctc gcccaggcgc agtccggcgc agtacgcgag cacgagcatc gtgtacaggc
     1681 tgagcggccg cagtgcggcc cgcggcgagg gccagtgcag cgcggtgtct agcagaagtc
     1741 gaacttcggc gggcgtgaac aagtgaggct gccgccgctc gactcggcgc cacagacgcg
     1801 gatccgggcg gggcattggc aggtcagggt cgagacggcg ccacgcccgg gcaaggtcgt
     1861 tcctgagcag ttggcattgg tatgcgtgag aggtggtcgg atgctcgccc gcccattgcc
     1921 tcagcaactc cgttggcgaa ctgcccgcaa ggtctggtcg accttgcaga aagcgatcga
     1981 agcgcaggaa ccgcgccgtc tgcgtgccgt actgacggcc caaggcacgc atcagttcgg
     2041 cgtggccgcg caggaagtcg cccaggaagc tggcaaacgg cggcaagcat cgaacggctt
     2101 cgagcgcctt gtgcgggtcc gtacagagca acgcgcgaac gatgggcggg gtcccccgca
     2161 gcccgtactg ggcgcgtaga tcagccaggg gattgaccgc gatgagacca tcgcggacga
     2221 gaaaatcgag aaaccgatcg agcacacgtg actgatgggc gacgagatcc tcattgcagt
     2281 gcctcgcgcg ctcgcgcagc cactccacga tgatcgtgag atcgacggtt ccgtcaccgc
     2341 gctgctccgc cagtcgctga aagccggcga ggatgcctcg atagattcga gccgtatgtg
     2401 aggtgcgcag gcgcagttgc cgcaaatagc ggccaatggc gtcgccgttg acatcaggcc
     2461 aggcgttcat gcttgaacct cctgcccggg gatctccagt gccacgtcac gcaggtcatc
     2521 ggtggcgagc ttgagatatg ggcgcgtcga ctcggctgag cggtggccca acagatcgcc
     2581 gatcgtcttg gtggttgcag atgcgcgaag aaggctgact gcgcgggcgt gccgaaaggt
     2641 atgcggtccg cacttgccgg cagggctgac ggcggcaccc tccaagcgca agcggaccag
     2701 caggtacagg ctggatccgc gactgaaaga tctatacgga gccttggtat ggacaaagat
     2761 ctcgcgggca tcggtgacgg gccgtcctcg cctcaagtag gccagcaggg catcgccgac
     2821 ggccgggacc aaaggcaggt ccgactggag gccggtcttg gtatgccgaa ccacgaatcg
     2881 gtcaccacgc cagtccacat cgtccaaccg cagttggacg atctccccgg cccgcaggcc
     2941 gtaggtggca agcagcacga ggatcgcgta gtcgcgcaat cccttggaag agtggtcctg
     3001 gccggtcgaa gcaagaacgg cgcgtatctg ttcgggatcc aggagcgagg gcatcgcctc
     3061 gcaggcgtac agcgttggcg aaatgacgcg ggcggcgagg tcgagcgcag tgcgcccggt
     3121 gacatgaagg aacttgagga agcaacgcag ttgctgcgtc acattcctgc gcgagacccg
     3181 gcgcagcgcg ggacacctcc actggacata ggcgtcgatg tcagcgagcc gcatggccct
     3241 gagatcgaac gcgtcggcgc tgcccccgta ccacgagaga agtcgtcgcg cttcggcgac
     3301 catgacgccg atggatcggg ccgcaagccc tcgctgcgca tgcagccact gagcgaactc
     3361 ggccagtagg ccgtcgaagt acaactcgga cggactgcgt gctgccggtg cgacgggcca
     3421 gctgcccctg acaacccgaa gcagttggtg gatgccggcg gtgtgcgaac tgcgccagcg
     3481 cgtcatggag gcggccgctc gtccgtgacg ttgacgatac cgacggagct tgcaggcaag
     3541 gtacatggag acgtgcgcag gctcgacctc atcgaggcga acatgtcgct tctccaagta
     3601 gcgaaggaag agcctggcga acgcgacgta gcgaacgatg acggttgggg cgtatcgcgc
     3661 caacgtcaaa tgttcccaga gctgggccag aggtgttgca gcatcgagcg ggcggtccga
     3721 catcatcggt tccttgggga gttggaggaa ccaaggatcg tgccgccgcc ggatcaacga
     3781 tccgtgccca accgactgaa gcgcggaaac cggtcgatgg cgatcccatc aacggtttgc
     3841 gcgggacgaa gcgcaaagtc cgccaaataa tgtgaagcaa acgccggcgc agatgccgcc
     3901 gctcccaggc gcccggcgcc ggtccttcac attacggagt cctgctgatt attgccggaa
     3961 tcgtcagctc gcgattccgg ccgcttggcg tagaccaccc actcggcctt gcgcagcggc
     4021 gccagccagg ccttgaactg cgctgcgccc gccaggccgg cgtgctcgcc gaagaactgc
     4081 aaccggccgg cctcatgcag ccgctgcaac tcctccagga accggcgccg gaacagccgc
     4141 gagagcacgc
//
LOCUS       DAOORV010000259         4135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_25584_length_4135_cov_12.010049, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000259 DAOORV010000000
VERSION     DAOORV010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25584_length_4135_cov_12.010049"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKV56_18660"
     CDS             <1..1046
                     /locus_tag="PKV56_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOX69869.1"
                     /translation="EVEIYIGILIGAVTFSGSLIAFGKLSGKIGGKPLLLPGRHLLNL
                     AGLLVVIWFGRVFLNSHDIQSGMLPLLVMTVIALLFGVHMVMAIGGADMPVVVSMLNS
                     YSGWAAAATGFMLSNDLLIVVGALVGSSGAILSYIMCRAMNRNFISVIAGGFGGGAPV
                     AKGDGAAAEPQGEVSPILAAETAELLRESKNVVIVPGYGMAVAQAQHTVYEITKLLRE
                     KGINVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDEINDDFPDTDVTMVIGANDI
                     VNPAAQDDPTSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDA
                     KKMLEEVLAALRS"
     gene            1165..1962
                     /locus_tag="PKV56_18665"
     CDS             1165..1962
                     /locus_tag="PKV56_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOX69870.1"
                     /translation="MSNTLVSSPPASPRVALFVTCLVDAMRPSVGFAALKLLQDAGCR
                     VEVPMQQTCCGQPAFNSGNTKDTAVLARRFIEQFEPYDYVVAPSGSCAGMTRAHYPEA
                     LADDPRWAERARKLGARTFELMSFLVDVMHYKPAGVKFPATATYHDSCSGLRELGVFD
                     QPRALLGQVGGLTMKPLEGNDVCCGFGGTFCVKYPAISNAVVSEKAAAIERTGAQVLL
                     GGDMGCLMNMAGKLHRNGSPVLVYHAAEVLAGMADAPSISARSSDKG"
     gene            1971..3386
                     /locus_tag="PKV56_18670"
     CDS             1971..3386
                     /locus_tag="PKV56_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOX69871.1"
                     /translation="MQTSVISFKARADAALADPTLKLAIDRTTGTAERKRAAALVDFP
                     QFEAARERGKRIKDHVIEHLDHYLLEFERNAIASGAKVHWATSADEASRIVVDLCRQA
                     KAKRVTRVKSMLGEEIGLPHALDAAGFERFETDLAEHIVQLAGDRPSHIVWPSMHRTR
                     EQVSVMFKKAHRVPHKDETIEAMVDSARRELRDKFLSADVSISGANFLIADTGATCTV
                     TNEGNAELTTSPPRMHIVTAGIEKLVPSLPHAFALLRLLVRSATGADVTQYTTFHCGP
                     KKPVDLDGPEEFHIVLVDNGRTKMLADAGLRDMLRCLRCGACMNHCVVFKQLGGHAYG
                     GVYPGPMGAVLTPVFDGLKESRELSHACTLNGKCQEVCPVDIPLPTLMRGWRDRSWRE
                     GLEPSSMRFGMGIFTFVASRPWIYRLGATVAVRMMRLFGRGGWIRGMPGLGPWTAHRD
                     FPVPSGRTFMARYASGEGRQK"
     gene            3383..4030
                     /locus_tag="PKV56_18675"
     CDS             3383..4030
                     /locus_tag="PKV56_18675"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOX69872.1"
                     /translation="MTARDDILGRVRAALGRRSIDPASVASAAATLLADLPSTRPRLL
                     ASSLAEAFIARVTSPKVAATAERIESTAALPEAVARYLRARDLPKAIALQPLALLRSL
                     DWSGFAVRDRVASDEVAAIGMARWGIAETGSLVFHSGADTAVLANFLPLHHIVLLRKA
                     DLLAYLEDYATLVAGQPMPRNVNLITGASGTTDIEGSLVLGAHGPRYMHVVLVDA"
BASE COUNT          682 a         1347 c         1392 g          714 t
ORIGIN      
        1 acgaggtgga gatctacatc ggcatcctca tcggcgccgt caccttctcg ggctcgctga
       61 tcgccttcgg caagctgtcg ggcaagatcg gcggcaagcc actgctgctg ccggggcggc
      121 atttgctcaa cctggcgggc ctgctggtgg tgatctggtt cggccgtgtg ttcctgaatt
      181 cgcacgatat ccagtccggc atgctgccgc tgctggtgat gacggtgatc gcgctgctgt
      241 tcggcgtgca catggtgatg gccatcggcg gcgccgacat gccggtggtg gtgtcgatgc
      301 tcaacagcta ctcgggctgg gcggcggcgg ccaccggctt catgctgagc aatgacctgc
      361 tgatcgtggt gggcgcgctg gtgggctcgt cgggcgccat cctcagctac atcatgtgcc
      421 gggcgatgaa ccgcaacttc atcagcgtga tcgccggtgg cttcggcggc ggcgcgccgg
      481 tggccaaggg cgacggtgcc gcggccgagc cgcagggcga ggtgtcgccg atcctggccg
      541 cggagacggc cgagctgctg cgcgaatcca agaacgtggt catcgtgccc ggctacggca
      601 tggcggtggc gcaggcccag cacacggtct acgaaatcac caagctgttg cgcgagaagg
      661 gcatcaacgt gcgcttcggc atccacccgg tggccgggcg catgccgggc cacatgaacg
      721 tgctgctggc cgaggccaag gtgccctacg acatcgtgct ggagatggac gagatcaacg
      781 acgacttccc cgacaccgac gtgaccatgg tcatcggcgc caacgacatc gtcaacccgg
      841 ccgcgcagga cgaccccacc agccccatcg ccggcatgcc ggtgctggaa gtgtggaaag
      901 caaaaacgtc catcgtcatg aagcgcagca tggccagtgg ttatgccggc gtggacaacc
      961 cgctgttcta caaagagaac aaccgcatgc tgtttggcga cgcgaagaag atgctcgaag
     1021 aagtgcttgc agcgctgagg agttgagcgc ttgagtacat tggagcgaca gggccgacac
     1081 cggtcttgcg gaaggcgacc cagatcgcgt caaagatgat cgaggcggtg gtccatcacc
     1141 gccacctccc gaacgaaagt gcgcatgagc aataccctgg tttcatcgcc gccggcatca
     1201 ccccgagtgg cgctgttcgt cacctgcctg gtcgacgcga tgcgcccgag cgtgggcttc
     1261 gcggcgctca agctgctgca ggacgcgggc tgcagggtcg aggttccgat gcagcaaacc
     1321 tgctgcgggc agcctgcgtt caacagcggg aacacgaagg acaccgccgt ccttgcccgg
     1381 cgcttcatcg aacagttcga gccctatgac tacgtggtcg ctccgtcggg ctcgtgcgca
     1441 ggcatgacgc gtgcccacta ccccgaggcg cttgccgacg atccgcgctg ggccgagcgc
     1501 gcgcgcaagc tcggcgcgcg caccttcgag ttgatgagct ttctcgtcga cgtgatgcac
     1561 tacaaacctg ccggcgtgaa gtttccggcc acggcgacct atcacgacag ctgctccggg
     1621 ctgcgtgagc tgggtgtctt cgaccagccg cgtgcgctgc tcggccaagt gggtggcctc
     1681 acgatgaagc cgctggaggg caatgatgtc tgctgcggat ttggcggcac gttctgcgtc
     1741 aagtacccgg cgatctcgaa cgccgtggtg tcggagaagg ctgccgcgat cgagcgcacc
     1801 ggtgcccagg tccttctggg tggagacatg ggctgcctga tgaacatggc cggcaagctg
     1861 catcgcaatg gttctccggt gctggtctac catgctgccg aggtgctggc gggcatggcc
     1921 gatgcgcctt ccatatcggc aagaagcagc gacaaaggat gagcgccgcc atgcagacct
     1981 ccgtcatctc gttcaaggcg cgcgcggacg cggcgctcgc tgatcccacg ctgaagctgg
     2041 cgatcgaccg cacgaccggc acggcggagc gcaagcgtgc cgccgcgttg gtggattttc
     2101 cgcagttcga ggccgcccgc gaacggggca agcggatcaa ggaccacgtc atcgagcacc
     2161 tcgatcacta cctgctcgag ttcgaacgca acgccatcgc ttccggtgcg aaggtgcact
     2221 gggcgacgtc ggccgacgag gcctcgcgca tcgtcgtcga cctgtgccgc caggcgaagg
     2281 cgaagcgggt cacgcgcgtg aagtcgatgc ttggcgagga gatcggtctg ccgcacgcgc
     2341 tggacgccgc cggcttcgag cgcttcgaga ccgatctggc cgagcacatc gtgcaactcg
     2401 cgggcgaccg accgtcgcac atcgtctggc cgtcgatgca tcgcacgcgc gagcaggtgt
     2461 cggtgatgtt caagaaagcg caccgtgttc cgcacaagga cgagaccatc gaggcgatgg
     2521 tcgacagcgc ccggcgcgag ttgcgcgaca agttcctcag cgccgacgtg tcgatctccg
     2581 gcgccaactt cctgattgcc gacaccggcg cgacctgcac cgtgaccaac gagggcaatg
     2641 ccgagctgac gacatcgcca ccgcgcatgc acatcgtcac cgccggcatc gagaagctcg
     2701 tgccgagcct gccgcacgcg tttgcactgc tgcgcttgct ggtgcgttcg gccaccggtg
     2761 ccgatgtgac gcagtacacc accttccatt gcggacccaa gaagcccgtc gacctggacg
     2821 gcccggaaga attccacatc gtgctggtcg acaacggccg cacgaagatg ctcgccgacg
     2881 ctggcttgcg cgacatgctg cgctgcctgc ggtgcggcgc ctgcatgaac cactgcgtgg
     2941 tcttcaagca gttgggcggc catgcctacg gcggcgtcta ccccgggccg atgggagccg
     3001 tgctcacgcc ggtgttcgac ggcctgaaag agtcccgtga actctcgcat gcctgcacgc
     3061 tcaacggcaa atgccaggaa gtctgcccgg tggacatccc gctgccgacc ttgatgcgtg
     3121 gctggcgcga ccgctcgtgg cgcgaaggct tggagccgtc gtcgatgcgg ttcgggatgg
     3181 ggatcttcac gtttgtcgcc tcgcgacctt ggatctaccg gctgggcgcg acggtagcgg
     3241 tccgcatgat gcgcttgttc ggccgcggcg gctggatccg cggcatgccc ggactcggtc
     3301 catggaccgc gcaccgggac tttccggtgc cttcaggccg caccttcatg gcccgctacg
     3361 ccagcggcga ggggaggcag aagtgacggc acgtgacgac attctgggcc gcgtccgcgc
     3421 tgcgctcggt cgccgaagca tcgatccggc aagcgttgcc agcgctgccg cgacgctgct
     3481 ggcggacctg ccgtcaacgc ggcccaggct cctcgcaagc tcgttggcgg aagccttcat
     3541 tgcgcgtgtc acctcgccca aggtggccgc gacggctgag cgcatcgaat cgacagccgc
     3601 actgcctgag gccgtggcca ggtaccttcg cgcacgcgat ctgccgaagg ccatcgctct
     3661 ccagccgctt gcgctgctgc gttcgctcga ctggtcgggg ttcgcggtgc gcgaccgcgt
     3721 cgcctctgac gaagtggccg cgatcggcat ggcgcggtgg ggtattgccg agaccggctc
     3781 gctggtcttc cattccgggg ccgacacggc ggtgctggcg aatttccttc cgctgcatca
     3841 catcgtgctg cttcgcaagg ccgatctcct ggcctacctt gaagactacg cgacgctggt
     3901 tgctggccag ccgatgcccc gcaacgtgaa cctcatcacg ggggccagcg gcacgaccga
     3961 catcgaggga agcctggtgc tgggcgccca tggcccgagg tatatgcatg tcgttctcgt
     4021 cgacgcctag cagggcggtt gcagtcggct gcgcgctcgt ttcgcattcg atggacaagc
     4081 gctcgaggcg acctggcgtt gactgccgag gccccgaccc gcatgtggcc ggccg
//
LOCUS       DAOORV010000260         4075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_26130_length_4075_cov_10.688557, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000260 DAOORV010000000
VERSION     DAOORV010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26130_length_4075_cov_10.688557"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="PKV56_18680"
     CDS             <1..2533
                     /locus_tag="PKV56_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOX69873.1"
                     /translation="VDGVRIGSATSGSPVLETIPLEVVERIEILRGPASALYGADALG
                     GVIQVFTRKGREGFQPSVRVGAGSNGAREGSVTRYGVVTKDGVGEAVEGLVLGLAGAN
                     AQKVVEGVTKKLEDIKPTLPKGVEIKVFYNRATLVEKAVGTVSKALMEATVLVLVLLG
                     AFLGNLRAAVTVAMVLPLSALATFILMRTTGMSANLMSLGGLAIALGMLVDAAVVVVE
                     NVVQHLSHDKASEKLPRLHVVFRAVREVAAPVAAGMLIIIVVFLPLLTLQGLEGKFFV
                     PVALTIVFALISSLVLSLTVIPVLASYLLKKVSHEDPWLPRQLLKLYEPALAFALKRQ
                     SVVYVAAALMLAVAAGVYTQVGKTFMPTMDEGDIIVGIEKLPSVSLEETAALDLKIHQ
                     ALMKAIPEITGVVARAGSDEIGLDPMGLNQTDTFLVLKPREAWQVQSKEALMDKIRGV
                     LDQLPGVAYSFTQPIDMRVSEMIIGVRGDVAIKVFGPDLTTLNDLAGQIEKLMKTVPG
                     NQDVYTVENDGVQYLRVVVDRLAAGRYGLSVEDVQDALRVQIEGQRAGTVITGGRRIP
                     ILLRGPDGVRVSPADFAALRISTADGQSVPLQSLAKLERDSGPVKIERELGSRYSVVI
                     ANVTGRDLVGFVEEARAKVAQEVKLPTGYRVAWGGQFENQQRAAARLILVVPASLGFI
                     FIILFSTFGSVRQALLVLSNIPFALVGGIVALWATGEYLSVPASVGFIALLGIAVLNG
                     VVLVSYFNQLHAEGLSLLDSVTQGAKRRLRPVLMTAAITAFGLIPLLFATGPGSEIQR
                     PLAIVVIGGLITATALTLILLPILYLRFAHPKPGAATAALEMNHV"
     gene            2526..2828
                     /locus_tag="PKV56_18685"
     CDS             2526..2828
                     /locus_tag="PKV56_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HOX69874.1"
                     /translation="MSEFCLVLVCPPSIEEKLLDALLVSAGNEIFTSTPTYSHGTAHG
                     RMANLEQVMGRSHSVKVEILVAEDEMARLLQTLRESFKGTGLRYWAWPLAAEGEIA"
     gene            2825..4072
                     /locus_tag="PKV56_18690"
     CDS             2825..4072
                     /locus_tag="PKV56_18690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX69875.1"
                     /translation="MKHWIAAAGMALALPLSWNAHAAGVVTPPDLPATEQARAAIDQD
                     PAVNQARHGVQAATHSAAMLEASPYEWTASTTAQRRRVDGSGTSNEWSVQLERTVRIG
                     GKAALDRDLGEAGRRLSTAQLGAVRNEAARQLLDLWLDWLAARQAREGLLEQVRFAEA
                     NISAVEKRRKAGDASMLELNVAKGDLAEVQRQVSALAAAEAKAQATLRVRYPQLPLEA
                     RALSEPLPPERNEGQWREQIAAGSDALRAAQQALRKAELTAARARADRMPDPTVGVFT
                     SSEAFRSERVIGLSLSIPLGGTYREQGAREALQQVEIARAGVERQQRDLDVQVAVQVG
                     EATSSFERWQLAERSATVAQESARLTQRAFTLGEADLQALLLVRRQAVDAVNAAVAAR
                     VDALRSRYRLLVDARLIWSLDEN"
BASE COUNT          667 a         1301 c         1430 g          677 t
ORIGIN      
        1 tgtcgatggg gtgcgcatcg gttcggccac cagtggttcg cccgtgctgg agacgattcc
       61 gcttgaagtc gtcgagcgca tcgagatcct gcgcggaccg gcgagtgcgc tctatggcgc
      121 agacgcgctc ggtggcgtga ttcaggtgtt cacccgcaag gggcgcgaag gcttccagcc
      181 ctcggtgcgc gtcggcgcgg gcagcaacgg tgcgcgcgaa ggcagcgtca cgcgctacgg
      241 cgtggtcacc aaggacggtg tcggcgaagc tgtggaaggc ctcgtactcg gcctggccgg
      301 cgccaacgcg cagaaagtgg tcgagggggt gacgaagaag ctcgaagaca tcaagcccac
      361 cttgcccaag ggtgtggaga tcaaggtctt ctacaaccgc gccacgctgg tcgagaaggc
      421 ggtgggtacg gtctcgaagg cactgatgga agccaccgtg ctggtgcttg tgctgctggg
      481 tgccttcctc ggcaacctgc gcgcggccgt caccgtggcg atggtgctgc cgctgtcggc
      541 gctggccacc ttcatcctga tgcgcacgac aggcatgtcg gccaacctga tgagcctggg
      601 cggcctggcc atcgcgctgg gcatgctggt cgatgccgcc gtggtcgtgg tggaaaacgt
      661 ggtgcagcac ctgagccacg acaaggcctc ggaaaagctg cctcgcctgc acgtcgtatt
      721 ccgcgccgtg cgcgaggtgg ccgcaccagt ggccgccggc atgctgatca tcatcgtcgt
      781 gttcctgccg ctgctcacgc tgcaggggct ggaaggcaag ttcttcgtgc cggtggcgct
      841 gaccatcgtg tttgcgctga tcagctcgct ggtgttatcg ctcaccgtca tcccggtgct
      901 ggcgtcctac ctgctgaaga aggtcagcca tgaagacccc tggctgccgc gccagctgct
      961 gaagctgtac gagccggcgc tagcctttgc gctgaagcgc cagtcggtgg tgtacgtggc
     1021 agccgcgctg atgctggcgg tggcggcggg ggtgtacacc caggtcggca agacgttcat
     1081 gccgaccatg gacgaaggcg acatcatcgt cggcatcgag aagctgccgt ccgtgagcct
     1141 ggaggagacc gcggcgctcg acctgaagat ccatcaggcg ttgatgaagg ccattccaga
     1201 gatcacaggt gtcgtggccc gcgccggatc cgacgagatc ggcctcgacc cgatgggtct
     1261 gaaccagacc gacaccttcc tggtgctcaa gccgcgcgag gcctggcaag tccagagcaa
     1321 ggaagcgctg atggacaaga tccgcggcgt gctcgaccag ttgcccggcg tggcctacag
     1381 cttcacccag ccgatcgaca tgcgcgtttc ggagatgatc attggcgtgc gcggcgacgt
     1441 ggcgatcaag gtcttcgggc ccgacctgac tacgctgaac gacctggccg gccagatcga
     1501 gaagctgatg aagacggtgc cgggcaacca ggacgtctac acggtcgaga acgacggcgt
     1561 gcagtacctg cgcgtggtgg tggacaggct ggcggccgga cgctacggcc tgtcggtcga
     1621 ggatgtgcag gacgccctgc gcgtgcaaat cgaaggccag cgtgcgggca cggtgatcac
     1681 cggcggcagg cgcatcccga tcctgctgcg cgggcccgat ggcgtgcgtg tatcgcctgc
     1741 agactttgca gcgctgcgca tcagcacagc tgacgggcag agcgtgccac tgcaatcgct
     1801 ggccaagctg gaacgcgaca gtggtccagt gaagatcgag cgggagctgg gcagccgcta
     1861 cagcgtggtc atcgccaacg tgactggccg cgacctggtc ggctttgtcg aggaggcgcg
     1921 tgccaaggtg gcgcaggagg tcaagctgcc caccggctac cgggtggcct ggggcggcca
     1981 gttcgagaac cagcagcgtg ccgccgcgcg gttgatcctg gtggtgcccg cgtcgctggg
     2041 tttcatcttc atcatcctgt tttcgacctt cggctcggtg cgtcaggccc tgctggtgct
     2101 gtcgaacatc ccgtttgcgc tggtcggcgg gatcgtggcg ctgtgggcca ccggtgagta
     2161 cctgtcggtg ccggcctcgg tgggtttcat cgcgctgctg ggcatcgcgg tgctcaacgg
     2221 cgtggtcctg gtgagctact tcaaccagct gcatgccgaa ggcttgtcgc tgctggacag
     2281 cgtgacccag ggcgccaagc ggcgcttgcg gccggtgctg atgacggcgg cgatcacggc
     2341 tttcggcctg atccccttgt tgttcgccac cgggcccgga tccgagatcc agcggccgct
     2401 ggccatcgtc gtcatcggcg ggctcatcac ggccacggcg ctgacgctga tcttgctgcc
     2461 catcctctac ctgcgcttcg cccatccgaa gcctggcgcg gccacggccg cactggagat
     2521 gaaccatgtc tgagttttgt ctggtactcg tgtgcccccc ctcgatcgaa gagaaattgc
     2581 tcgatgcgct gctcgtcagc gcgggcaacg agatcttcac gagcacgccc acctacagcc
     2641 acggcacggc ccacggccgc atggccaacc tggaacaggt catgggccgc agccactcgg
     2701 tgaaagtcga aatcctcgtc gcagaagacg agatggcgcg gctgctgcag accctgcgcg
     2761 agtcgttcaa aggcaccggc ttgcgctact gggcctggcc cctggccgcc gaaggagaaa
     2821 ttgcatgaag cactggattg cagcagccgg catggcgctc gccctgccgc tgagctggaa
     2881 cgcgcacgcc gcaggcgtgg tcaccccacc cgacctgccg gcgaccgagc aggcgcgtgc
     2941 agccatcgat caggacccgg cggtcaacca ggcccggcat ggtgtgcagg cggccacgca
     3001 ctccgccgcc atgctggagg cttcgcccta cgagtggacg gccagcacga cggcccagcg
     3061 gcgacgcgtc gacggcagcg gcacgtccaa cgagtggtca gtgcagttgg agcgcaccgt
     3121 gcgcatcggt ggcaaggctg ccctggaccg cgacctgggc gaggccggtc gcaggctatc
     3181 gaccgcgcaa ctcggcgcgg tgcgtaacga ggctgcgcgc cagttgctcg atctctggct
     3241 ggactggctg gctgcgcggc aggcccggga gggcttgctg gagcaggtca ggtttgcgga
     3301 agcgaacatc agcgctgtcg agaagcggcg caaggcgggc gatgcctcga tgctggaact
     3361 caatgtcgcc aaaggcgacc tcgccgaagt gcagcgccag gtcagtgccc tggctgcggc
     3421 agaagccaag gctcaggcga cgctgcgggt gcgctacccg cagttgccgc tggaagcacg
     3481 tgccctgtct gagccgctgc cgccggagcg caacgagggg caatggcgcg aacagatcgc
     3541 cgcgggcagc gatgcactgc gtgccgcgca acaggcgctg cgcaaggcgg aactgactgc
     3601 ggcccgagcg cgggctgacc gcatgcctga tcccaccgtg ggtgtcttca cctcgtcgga
     3661 agccttccgc agtgagcgag tcatcggtct gagcctgagc atcccgctgg gcggcaccta
     3721 ccgggaacaa ggcgcgcgcg aggcactgca gcaggtcgag atcgcacgcg ccggcgtcga
     3781 gcgccagcag cgcgacctgg acgttcaggt cgcagtccag gtcggcgagg ccaccagcag
     3841 cttcgagcgc tggcaactgg ctgagcgctc ggccaccgtg gcgcaggagt cagcacgtct
     3901 gacccagcgc gccttcacac tgggcgaagc cgacttgcag gccttgctgc tggtgcggcg
     3961 ccaggccgtg gacgcggtca acgccgcagt ggctgcacgc gtcgatgcct tgcgctcccg
     4021 ctaccgactg ctcgtcgatg cgcggctgat ttggagcctc gatgagaact gatcc
//
LOCUS       DAOORV010000261         4063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_26236_length_4063_cov_11.021707, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000261 DAOORV010000000
VERSION     DAOORV010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26236_length_4063_cov_11.021707"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1266)
                     /locus_tag="PKV56_18695"
     CDS             complement(154..1266)
                     /locus_tag="PKV56_18695"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX69876.1"
                     /translation="MQPRDMRPADLLAAVLTGRASPPASDDGWLALLSQARRANLAAR
                     LAQQLHASGITVPPQTRWQLDGAVKAQRHLARLMALELRRVAQALRSDGVGFVLLKGT
                     AYLAAGLPAASARQFGDIDLLVRQADLAAAEAALMGGGWVNLDASPYDQRYYREWMHE
                     IPPLTHISRGSVIDLHHTIVPPTSVFRVDGADLLAHALPVADSQGLQVLQPTDMVLHS
                     MVHLFTDGEFDNGLRDLLDLWDLLRHFNQHDAGFWDALLARAQVLGLGRPLHHALHHL
                     ERLAGPTVPDRLRPQLQLLQPPLPARWLMQWLLRVGLRPQHPACRQPGDGLARWLLYV
                     RSHWLRMPLRLLLPHLLRKAWVQRFPVKDGKEQAAA"
     gene            complement(1270..2181)
                     /locus_tag="PKV56_18700"
     CDS             complement(1270..2181)
                     /locus_tag="PKV56_18700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HOX69877.1"
                     /translation="MAVSATSAAQSLRSGGLLLQTGPFTFRIRSPHAGVARNFNLLYA
                     NHASLAQSAPVDFDVQISDGAAWRRYWRRQACFVFDGRAVFEPLPAAHAFALLEWAMN
                     WCISTHAHQYLIVHAAVLSRDDRALILPAPPGSGKSTLCAALTHSGWRLLSDELTLID
                     MRDGRIWPLCRPVSLKNASIQVVRQFAPQAVFGEVTRDTAKGDVMHMAVPVEHLRQVQ
                     QPALARWIVYPRYQAGADTCLQPRPRAGAVVDLARNAFNFHFQGDAGFEMLCDVVEGC
                     DCFDFRYSRLPEAIAAFDALANDTPGA"
     gene            complement(2195..2431)
                     /locus_tag="PKV56_18705"
     CDS             complement(2195..2431)
                     /locus_tag="PKV56_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69878.1"
                     /translation="MLDGVWLVHSSASGATMAADAVDAALLELLGDGPVSATLLTRRL
                     SDAVQVLLPPDAVVGVESHLQGLLQHGWLVVLDD"
     gene            complement(2522..3370)
                     /locus_tag="PKV56_18710"
     CDS             complement(2522..3370)
                     /locus_tag="PKV56_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69879.1"
                     /translation="MNTTPQPDPRSSIPPTEEEALADRRRAILRGLGKGGVALAALSP
                     VVSHATRSFKVKNPDLLPASEFGYCTVSGFQSAAISGSPTAVECGAFEPDKFVGPLIS
                     LDYDALIATHASTVPAASTYRVKLATALNGYYGTGTNLTGPNIGSTLRATPAVKLVIN
                     TTNTKTVIVPGLLGGAASRTSGTELRPTTDFPQSVNPTQSFNSLFPSSSDARTLLEVL
                     QDGVASNNPTSANCYVLATYLSVGASGASLPPSLDRAYIQAQYTASSYGSSTNLYKFF
                     RSVCLG"
     gene            3683..>4063
                     /locus_tag="PKV56_18715"
     CDS             3683..>4063
                     /locus_tag="PKV56_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69880.1"
                     /translation="MIRVGNSRQLQRVTLGLVTAFGLGWASSPAMAQSAVANQNAGLV
                     KRAAGVVSVVRNGVPLLVQPGLPVQVGDTFRTGPDSAVALALADDTLLSVGPDSELQV
                     TAFAFDTTTQDGNLLASVWRGTVAF"
BASE COUNT          792 a         1305 c         1330 g          636 t
ORIGIN      
        1 ccggaactgg tggcgcggta tgcggcgccg cggctgcggg ggccgttcaa tctggcggcg
       61 cgcagggcgg cggggttcag tgacggggaa ttgcgacaat ttcttggcag cgcatgatgg
      121 ccgccgccgt tgggccatgg cgcagagcgg ctgtcaggca gcggcctgct ccttgccgtc
      181 cttgaccggg aagcgctgca cccacgcctt gcgcagcagg tgcggcagca acagccgcag
      241 cggcatgcgc agccagtgcg agcgcacata cagcagccag cgcgccaggc catcgccagg
      301 ctggcggcag gccgggtgct gcggacgcaa gcccacccgc agcaaccact gcatcagcca
      361 gcgcgcaggc agcggtggct gcagcagctg caattgcggg cgcagacggt ctggaacagt
      421 gggcccggcc aagcgctcca agtggtgcag cgcatggtgc agcggccgac ccagccccag
      481 cacctgggca cgcgccagca acgcgtccca gaagcctgca tcgtgctggt tgaaatggcg
      541 cagcaagtcc caaaggtcga gcagatcgcg cagaccgttg tcaaattcac cgtcggtgaa
      601 cagatgcacc atgctgtgca gcaccatgtc agtcggctgc agcacctgca ggccctggct
      661 gtccgccacg ggcagcgcat gcgccagaag atcggcgcca tcgacccgga acaccgaggt
      721 gggcggcacg atggtgtggt gcaggtcgat caccgagcct cggctgatgt gggtgagcgg
      781 cgggatctcg tgcatccact cgcggtagta gcgctggtca tacggtgagg catcgaggtt
      841 gacccagccg ccgcccatca gcgcggcctc agccgctgcc aggtcagcct gtcgaaccag
      901 caagtcgata tcgccgaatt gccttgcact ggcagctggc aggcccgcgg ccaggtaggc
      961 agtgcccttg agcagcacga atcccacgcc atcgctgcgc agcgcctggg cgacgcggcg
     1021 cagttccagt gccatcagcc ttgccaggtg gcgctgggcc ttgaccgcgc catcaagctg
     1081 ccagcgcgtc tgcggcggca cggtgatgcc gcttgcgtgc agttgctggg ccagcctcgc
     1141 cgccagattg gcccgcctgg cctgggacag caacgccaac catccgtcgt ccgacgctgg
     1201 cggcgatgcg cggccagtga gcactgcggc cagcagatcg gcaggtcgca tgtcccgtgg
     1261 ttgcatcgct cacgctcccg gcgtatcgtt ggccagcgcg tcgaacgcgg ctatcgcctc
     1321 gggcagccgg ctgtagcgga agtcgaagca gtcacagcct tcgaccacat cgcacagcat
     1381 ttcaaagccg gcatcgccct gaaagtgaaa attgaaagcg ttgcgtgcca agtccaccac
     1441 cgccccggcg cggggccgag gctgcaaaca ggtgtcggcg ccagcctggt agcgggggta
     1501 gacgatccac cgcgccagcg ccggctgctg cacctgccgc aaatgctcca ccggcacagc
     1561 catgtgcatc acgtcgccct tggcagtgtc gcgcgtgacc tcgccgaata cggcctgcgg
     1621 cgcaaactgc cggacgacct ggatcgaggc gttcttcagg ctgaccggcc ggcacagcgg
     1681 ccagatgcgg ccgtcgcgca tgtcgatcag cgtcaattca tcagacagca accgccaacc
     1741 gctgtgggtg agcgcagcgc acagcgtgct cttgcccgag ccaggcggtg ccggcaagat
     1801 cagcgcgcgg tcgtcgcgcg acagcaccgc cgcatgcacg atcagatact ggtgggcatg
     1861 ggtagagatg caccagttca tggcccactc cagcagcgca aaggcatgcg cggcggggag
     1921 cggctcaaaa acggcccgac cgtcgaaaac aaaacaggcc tggcgtcgcc agtagcggcg
     1981 ccaggcggcg ccgtcggaga tctgcacgtc gaaatcaacc ggagcactct gggccaggga
     2041 ggcgtggttg gcgtagagca ggttgaagtt gcgcgcaacg ccagcgtgcg gcgagcgaat
     2101 ccggaatgtg aacgggccgg tctgcaaaag caaaccgcca gaacgaaggc tttgtgccgc
     2161 agaagtcgcc gaaacagcca tcagtgcacc tggttcaatc atccaggacg accagccagc
     2221 catgctgcag caatccctgc aggtgagatt caacgcccac tacggcgtct ggcggcagca
     2281 agacttgaac agcatcggag agccggcgcg tgagaagggt ggcggacacc ggaccgtcgc
     2341 cgagcaattc caacaaggca gcgtccacgg catcagcggc catcgtggca ccgcttgcac
     2401 tgctgtgcac caaccagacg ccatccagca accgccagcg caaattgccg actagcgcat
     2461 aacgcctttg cggcgccgaa gcctcagcag tcacatgcgg aatggcgccg cgtgcaatcg
     2521 gtcaaccaag gcacaccgat cggaaaaact tgtacaggtt cgtcgatgac ccgtaactgg
     2581 aggctgtgta ctgcgcctgg atgtaggcgc ggtccaacga cggcggcagg gaggcgccac
     2641 tcgcaccgac ggacagatac gtcgcaagca cgtagcagtt cgcagacgtt gggttgttgc
     2701 tcgcaacacc atcctgcagc acttcaagca acgtgcgggc atctgagctc gacggaaaga
     2761 gcgagttgaa cgattgggtc ggattgacgc tttgcggaaa gtcggtcgtc ggcctcaact
     2821 cggtgccaga agtacgagaa gctgcaccac ccagcaggcc tgggacaatc accgttttcg
     2881 tgttcgttgt attgatgacg agtttgactg ctggagtggc acgcagtgtg gacccgatat
     2941 tcggccctgt cagatttgtc cctgtgccgt aataaccgtt gagtgcggta gcgagcttga
     3001 cccggtacgt gctggcggca ggcacagtcg aagcatgggt agcaatgagc gcgtcatagt
     3061 ccagcgaaat caggggcccg acaaatttat ccggctcaaa cgcaccgcac tccaccgctg
     3121 tcgggctgcc ggaaatggcg gccgactgga atccggacac ggtgcagtaa ccaaattcgg
     3181 acgcgggcag cagatcgggg ttcttcactt tgaaggaccg cgtggcatgg ctgaccaccg
     3241 gagacagcgc cgccaatgcg accccgccct ttcccagccc tctcaagatg gcgcgccgcc
     3301 ggtctgcgag agcctcttct tcagtcggcg ggatggagct gcgaggatcg ggctgagggg
     3361 tggtgttcat gtcacatcct gactcttgat ggtgcaagcg gacgcagcga atgccgcgcc
     3421 tgttccgcga tgcatcgaag atatcagcaa accatggggg ttcccgtgaa caggcgcccc
     3481 actccccccg aactgaggtg tgaacaattt ccggctgtag ccagggattg acgatgcggc
     3541 tgcgtcgaca gactaagagg acgaaacccg gcgtgcgcgg ggtgcgcgac gcgtcgatgg
     3601 tggcagtcgc ggccacacac acgcacaaca tgcggcacat ccggccaggc gcttcggccc
     3661 agcgggccaa tacactcgct agatgatcag ggtcggcaat tcacgccaac tgcaacgtgt
     3721 cacgttgggg ttggtcaccg catttggcct gggatgggcc agcagcccag caatggcgca
     3781 gtcggcagta gccaaccaaa acgcaggcct ggtcaagcgt gcggcaggtg tggtgtcggt
     3841 ggtgcggaac ggcgtccctc tccttgtaca gcctggcctg cctgtccagg ttggcgacac
     3901 cttccgcacc ggccccgaca gcgccgttgc gctggcactc gccgacgaca ccttgctgtc
     3961 tgtcggcccc gacagcgaac tgcaagtcac cgcgttcgcc ttcgacacca ccacccagga
     4021 cggcaacctg ctggcttcgg tctggcgcgg caccgtcgcc ttc
//
LOCUS       DAOORV010000262         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_26527_length_4032_cov_6.445562, whole genome shotgun sequence.
ACCESSION   DAOORV010000262 DAOORV010000000
VERSION     DAOORV010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26527_length_4032_cov_6.445562"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PKV56_18720"
     CDS             complement(<1..1098)
                     /locus_tag="PKV56_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOX69881.1"
                     /translation="MNKILLQIATELKVRPAQVNAAVELLDGGATVPFIARYRKEATD
                     NLDDIQLRELEARLSYLRELEERREAVLKSIEEQGKLTPELRASIEAAPTKQELEDLY
                     LPFKQKRRTKAMIAREAGLEPLADKLFADPTLDPLAEAAAFLNADGGFADAFAVLDGV
                     RDILSERWAEDAVLVGKLREWLWSEGQLQSRLLDGKDANQPDTAKFRDYFDYAEPIRN
                     VPSHRALAVFRGRTLELLDAKLVLDEAPSAVAPPTAGKAAPVVSLAEGRIAVHLGWSH
                     AKRPADDLIRKCIAWCWKVKLSLSLERDLFSRLRESAEQVAIKVFAENLRDLLLAAPA
                     GPRVVMGLDPGIRTGCKVAVVDATGKVLATET"
     gene            complement(1235..2518)
                     /locus_tag="PKV56_18725"
     CDS             complement(1235..2518)
                     /locus_tag="PKV56_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HOX69882.1"
                     /translation="MSPPSVAILGGGPAGLMAAEVLASAGALVDLYDAMPSVGRKFLL
                     AGRGGLNLTHSEAAEHFAARYGERRAPIESMLRRFGPAELRAWAQGLGISTFVGSSGR
                     VFPTDLKAAPLLRAWLHRLRELGVRFHMRHRWVGWPEGGAADAPVALAFETPAGQRTV
                     RADAVLLALGGASWSRLGSDGAWVPWLRSRGVELAPLRPSNCGFDVARFDAGGIEQSG
                     WSEHFRSRHAGAPVKNVALGCAGPAGRMFAQAGEFVVTEGGVEGSLIYAASAGLRDAI
                     ASDGRAVAHIDLLPGRSAEWVVREVAHPRGSRSLSTHLKGRLGIDGVKAALLHELLPR
                     EAMADPARLAASIKALPLTVTAPRPIDEAISTAGGVRFEALDDQGMLLALPGVFCAGE
                     MLDWEAPTGGYLLTASLASGVWAGHGLLQYLTARR"
     gene            2907..3548
                     /locus_tag="PKV56_18730"
     CDS             2907..3548
                     /locus_tag="PKV56_18730"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HOX69883.1"
                     /translation="MVIELYTSEGCNSCPPADRWLSQWGSQPEVIALAFHVDYWDHLG
                     WKDPFGSPAHTERQSQQRAVNGSRFSYTPQITINGVDSRQWRDEGPPMAGAAVASPLR
                     VLLTREGERYTALLQPGAGTPKRLAAYWTVTEDRLVSAVKSGENQGTTLTHDFVVREY
                     KSVPIWTTDFETASTLTFSPARAAGSAVHPRRVNLVIVNAASGRPVQGVSLGC"
BASE COUNT          728 a         1401 c         1309 g          594 t
ORIGIN      
        1 ggtttcggtg gccagcacct tgccggtggc atcgaccacc gcgaccttgc agccagtgcg
       61 gatgcccggg tccaggccca tcacgacgcg tggcccggcg ggagcggcaa gcaacaggtc
      121 gcgcaggttc tctgcaaaca ccttgatggc cacctgctcg gcggattccc gcaggcggct
      181 gaacagatca cgttcgaggc tcaggctcag cttgaccttc cagcaccacg cgatgcactt
      241 gcggatcagg tcatcggccg gtcgcttcgc atggctccag cccaggtgca ccgcgatgcg
      301 gccttcggcc agcgacacca caggcgccgc cttgcccgct gtgggcggag cgaccgctga
      361 cggcgcctcg tcgagcacca gcttggcatc cagcagttcg agtgtgcgac cgcgaaacac
      421 cgcgagcgct cgatgcgacg gcacgttgcg gatcggctcg gcgtagtcga agtagtcgcg
      481 gaacttcgcc gtgtcgggct ggttggcatc cttgccgtcg agcagcctgc tctgcagctg
      541 gccctcggac cacagccact cgcgcagctt gcccaccagc accgcatcct ctgcccagcg
      601 ctctgacagg atgtcgcgca ccccatcgag cacggcgaag gcatcggcga acccaccgtc
      661 tgcattgagg aaggcggccg cctcggccag cggatcgagc gtcggatcgg caaacagctt
      721 gtcggccagt ggctcgaggc cggcttcgcg ggcgatcatg gccttggtgc ggcgcttctg
      781 cttgaacggc agataaaggt cctcgagctc ctgcttggtc ggcgcagcct cgatcgacgc
      841 acgcaattcg ggtgtcagct tgccctgctc ctcgatgctc ttcagcaccg cctcccggcg
      901 ctcttccagc tcgcgcagat aagacagccg cgcctcgagt tcccgcaact ggatgtcgtc
      961 caggttgtcg gtggcctcct tgcgatagcg cgcgatgaac ggcaccgtcg cgcctccgtc
     1021 gagcagttcg acggcagcgt tgacctgggc gggccgaacc ttcagttcgg tagcgatttg
     1081 cagcaggatc ttgttcaagg cgtgggaccc gtcgaacggg tggaagcggg ggtgggatcg
     1141 ggtgcggaaa ccggctgaga acagcgggct cgcgccaggg ccgacgcggg ctcgaggccc
     1201 ggggcggcgc agtgtgccac aggggtgggt tggttcagcg ccgcgccgtc aggtactgca
     1261 acagaccgtg accggcccac acgccgctgg ccaggctggc ggtcagcaga tagcccccgg
     1321 tcggcgcctc ccagtcgagc atctcgccag cacagaacac gccgggcagc gccagcagca
     1381 tgccctgatc gtccagcgcc tcgaagcgca caccgccggc ggtgctgatg gcctcgtcga
     1441 tcgggcgcgg cgcagtcacg gtcagcggca gcgccttgat ggacgccgcg agccgtgcgg
     1501 gatcggccat cgcctcccgg ggcaacagct cgtgaagcag cgcggccttg accccatcga
     1561 ttcccagccg gcccttcagg tgcgtggaca gcgatctcga cccgcgtggg tgcgccacct
     1621 cgcgcaccac ccattcggcg ctgcgaccgg gcagcagatc gatgtgcgcc accgcgcggc
     1681 catcggaagc gatggcatca cgcagcccgg cagacgcggc atagatcagg ctgccctcga
     1741 cgccgccttc ggtcacgacg aactcgccgg cctgcgcgaa catgcggcca gccggaccgg
     1801 cgcagccgag cgcgacgttc ttgaccggtg cacccgcatg gcgactccga aagtgctcgc
     1861 tccagccgga ctgctcgatg cctcccgcat cgaagcgcgc cacgtcgaac ccgcagttcg
     1921 atggccgcag cggagccagt tcgacaccgc gcgagcgcag ccacggcacc caggcaccgt
     1981 cggatccaag gcgcgaccag ctcgcgccac cgagcgccag cagcaccgcg tcggcccgca
     2041 cggtccgctg gccggccggt gtctcgaagg ccagcgcgac cggtgcatcc gccgcgccgc
     2101 cctcgggcca gccgacccag cggtgcctca tgtgaaagcg cacgcccagt tcgcgcaggc
     2161 gatgcagcca ggctctcagc agcggggcag ccttcagatc ggtcgggaag acccggcccg
     2221 agctgccgac gaaagtggaa attcccaggc cctgcgccca ggcacgcaat tcggcaggac
     2281 cgaatcggcg caacatcgac tcgatcggtg cacgtcgctc gccataccgg gcggcgaagt
     2341 gctcggcggc ttcggaatga gtcaggttca acccgcccct tccggccagc aggaacttgc
     2401 ggcccaccga cggcatggcg tcgtacaagt ccaccaaagc cccggcagac gccagcactt
     2461 cagccgccat gagaccggcc ggccccccgc ccaggatggc gacggagggt ggggatatcc
     2521 gatgtgaatc aggcaagtga acgatccgat gtgataagtg gctggatctg gcccgacagc
     2581 gtaaagggac gtgagcggcc acggcagcca tgcagcatag ggcacggctt gacgttggtt
     2641 cggtgctcac cgccatgcag cgcaggaggt cgccacggta aggatctctc gacactgccc
     2701 gaccaagggg gacggtcaat ccacgggaaa ttgccatgaa gcccacgcca ccaccatcga
     2761 agcaacaacg cggccaagcc aggcgcctgt ggccgcactc cgcgcgttgg ctcgttgcag
     2821 cgctggctgc gggcggcctg ccggacgcag caaggactgc cgagcccctc gctagttgct
     2881 ccgcacgcag tggagccaac gcacccatgg tcatcgagct ctacacctcg gaaggctgca
     2941 attcctgccc gcccgccgac cgctggctgt cgcaatgggg ttcacagccc gaggtcatcg
     3001 cactcgcctt ccatgtcgac tactgggacc atcttggctg gaaggacccc ttcggcagcc
     3061 cggcgcacac cgagcgacag tcccagcagc gggcggtgaa cggctctcgg ttcagctaca
     3121 cgccgcagat cacgatcaac ggcgtcgaca gccggcagtg gcgggacgag ggcccgccga
     3181 tggccggagc ggcggtggcg tctccgctgc gggtcctgct gacccgtgaa ggcgagcgct
     3241 acaccgccct gctgcaacca ggagcaggca ccccgaaacg cctcgcggcc tactggacag
     3301 tcaccgaaga tcggcttgtg tcagccgtga aatccggaga gaaccagggc accaccctga
     3361 cacatgattt cgttgtccgc gagtacaagt ccgtaccgat ctggacgacg gacttcgaaa
     3421 cggcaagcac cctcaccttc agtccagcac gagcggctgg gtcagcggtt cacccacggc
     3481 gggtgaacct ggtgatcgtc aatgccgcga gcgggcgacc cgtgcagggg gtcagcctgg
     3541 gatgctgaga atcagagccc aacgatcggg actcgcgacc aggagatttc gattcgcgac
     3601 cgaaacggcc gagggtcggc aaaccggccc accacggctc aacaagcgac accgtggccc
     3661 ctgatgtgtc cctacagtca ggtgatctgc tcggtttgct ggcggatcac ttccttgacc
     3721 aagtcgccca acgaagccgt gccgatggcc cttacgtcct cgcggaccgg caggtgacgg
     3781 tgatcacttc gctggcgaga atgtcggatg ccaaggttca ggtggcttcc agacacttca
     3841 gaagcaccct cagggtgcag tcgctctcgg acacgcgccg cattcccggg attccgctga
     3901 tgcagacgcg atggagccgc gtccgccacg tgcaatagct cacgctctca cagtgcgaaa
     3961 gcccggagaa gacgggaaaa ccaaccccga agtgccgctc aagtcagacc agcgggatgc
     4021 cgacatcaca ga
//
LOCUS       DAOORV010000263         3928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_27471_length_3928_cov_6.402789, whole genome shotgun sequence.
ACCESSION   DAOORV010000263 DAOORV010000000
VERSION     DAOORV010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27471_length_3928_cov_6.402789"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1450)
                     /locus_tag="PKV56_18735"
     CDS             complement(176..1450)
                     /locus_tag="PKV56_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HOX69884.1"
                     /translation="MLLAHRIRLDPNNVQATHLGQAAGTARFAFNWGLAEWERQYKAW
                     KAGGQELPTEAALRRQLNAIKREQFPWMLEVTKTAPQMAIIQLGEAFKNFFAGRARYP
                     RFRRKGVHDRFTLTNDKFAVDGCRIRIPKLGWMRMREALRFTGRIMSATISRVADRWF
                     VSITVDTDELSHLPAAENQGAVGVDLGVLALATLSTGEVIAGPRPHKALLGRLRRLSR
                     GLSRKANGSANRKKARAKLARLHSRIATIRAAALHRLTTDLTRRFHTIGIENLNVRGM
                     LANRSLAGSIADMGFFEFRRQLEYKAAMRGGQVVLADRFFASSKTCSACGHKVDQLPL
                     ALRSWSCAYCGTTHDRDVNAAVNLRNVAVSSTAPACGEEGSGRGRKTLAKPASGKQEA
                     TSESGANRPVRASVAERCGTEPHAPIGKRQQG"
     gene            complement(1489..>3928)
                     /locus_tag="PKV56_18740"
     CDS             complement(1489..>3928)
                     /locus_tag="PKV56_18740"
                     /inference="COORDINATES: protein motif:HMM:NF023898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HOX69885.1"
                     /translation="GVPLLGDPFFMPTEVVASQVAPATGQLTAETGLPPRRRGRPPGA
                     KTRLLREGCGELGRHHFAFLRALLDGVDLERAWRLYLSFTGGPSDRRHFAGRLRLVVG
                     AIERAGATRECKAELAVALPALRALPDFAVARTKRGAGKAGHIDPEGMQRPAPSPPPS
                     PSLEEWRSQYCGAAGIDEDFYTEAEWLELYGEEFGAEAARGQFGAGKAGHFAALPSPA
                     LAATTHARLVEESPIAATSRLSGAQRKAVVEALATLERVISREASLEDEAGFWLGSGI
                     PRDLAKVGVKSLGDLVNFINIYGFRWHRRVPRMGVVRARRLLDWLTPIAEAGGRPFKE
                     SARRPETDLALLRRRDLAGRGVTNAEPFALVPIVDGALPDALSGRQGAFRSHEANVWG
                     AETDVQAISQWLSRYQGQTRRDYTRIAERFYLWCIHVRRKALSSLQEPDLQAYQAFLA
                     APPADWVQERKVRREDASWRPFRGPLGRNSQRHEFTVLGSMFSAMHEKGYLRANAMAN
                     LGRTLGLLKPSIDVRRSLSERQWAFAMEVLHEKPDTPERRRLQLLLELGSTTGLRLSE
                     LATTRLRGFRRELVDGEEAWLLDVIGKGGKLRTVMVFDEIKTLLEQHHQDMDAAGLGF
                     DARVARVQAPNSLPITAAGERMSATSQGLDDGIAVGAGQALQDALRSEHQEAWRPLVG
                     ILKRPPPRRDLDRLGVPFIDHQQPSQADRYGALERSAIYKVLRRFFRDVAVAAAARDG
                     APGSADFLNASTHWLRHTFANSAVKQMQPQVLQSLLGHSDLRVTSVYVKADAADLVRG
                     MRAMQRAAEGTQPK"
BASE COUNT          644 a         1313 c         1283 g          688 t
ORIGIN      
        1 gaggaagctc ttcgaaaact gctttcggag cttccggacg ccccctgatc agcctccgtg
       61 gtacggtacc acggagccct tgggagcacc gtttcgttgg cctcttgtgc cctcgggagg
      121 tgcctctcgg gctcgccaaa aatgcgtgcg tggtaccgta ccacgcgtgc ccgaatcacc
      181 cctgctggcg cttgccgatt ggggcgtgtg gttccgtacc acaccgttct gcaacacttg
      241 cgcgaaccgg gcgatttgcg cctgactcgc tggttgcttc ctgcttcccc gaggccggtt
      301 tcgctagggt cttgcgaccc cggccagagc cttcctctcc gcaggctggc gcggtggaac
      361 tcaccgctac attccttagg ttcaccgcgg cgttcacgtc gcggtcgtgc gtcgtgccgc
      421 agtaggcaca cgaccaactg cgcaatgcca gcggcaactg gtcgaccttg tgcccgcagg
      481 ccgagcacgt cttgctgctg gcgaaaaagc ggtcggccaa tacaacctgc ccgcctcgca
      541 tggcggcctt gtattccaac tgccgccgaa actcgaagaa gcccatgtcg gcaatggagc
      601 ctgccaggga acggttggcc agcatgccgc gcacgttcag attctcgatg ccgatggtgt
      661 gaaaccgccg cgtcaggtct gtcgtgagcc tgtgaagggc ggcggcgcgg atggtcgcga
      721 tccggctatg cagacgtgcc agcttggccc tggccttctt ccggttggcc gacccgttcg
      781 ccttgcgaga caggcccctg gagagccgcc tcagacggcc taagagggcc ttgtggggcc
      841 tcgggccggc aatcacctct ccagttgaaa gcgtggcgag cgccaacacg ccgagatcga
      901 cccccaccgc gccttggttc tcggctgcgg gaaggtgcga aagttcgtcc gtgtccacgg
      961 tgatgctgac gaaccagcgg tcggccacac gggagatcgt cgccgacatg atccgccctg
     1021 tgaagcgcaa cgcctctctc atccgcatcc aacccagttt cggaatgcgg atccgacagc
     1081 cgtcgactgc gaacttgtcg ttggtgagcg tgaagcggtc gtgcacgccc tttctgcgga
     1141 agcgtggata gcgagcccgg ccggcgaaga agttcttgaa ggcctcgccc aactggatga
     1201 tcgccatctg cggcgcggtc ttggtgactt ccagcatcca agggaactgc tctcgcttga
     1261 tcgcgttcag ctggcggcgc aacgctgcct cggtgggcaa ctcctggccg cccgctttcc
     1321 aagccttgta ctgccgttcc cactcggcca gtccccagtt gaacgcgaag cgcgctgtgc
     1381 ctgcagcctg gcctagatgg gtggcttgga cgttgtttgg gtcgagcctg atgcgatggg
     1441 caagcagcat gactggtcat tttccccagc caaatgcgtt gtcaagggct acttcggctg
     1501 ggttccttcc gccgctcgct gcatcgcccg cattcccctg accaggtctg cggcgtcggc
     1561 cttcacgtag accgacgtca ccctgagatc tgagtgcccc agcaggctct gcagcacctg
     1621 gggctgcatc tgcttcacag cgctgttggc gaaggtgtga cgcagccagt gggtcgaggc
     1681 attcagaaag tcagccgaac cgggggcgcc atcacgcgct gcagcggcga cggccacgtc
     1741 gcgaaagaag cgacggagca ccttgtagat cgccgagcgc tccagcgcgc cataccggtc
     1801 agcctgagat ggctgctggt ggtcaatgaa tggcacgccg agccggtcca aatcacggcg
     1861 agggggaggg cgcttgagga tcccaacgag aggacgccag gcttcctggt gctcgctgcg
     1921 caaggcgtcc tgcaatgcct gccccgcacc cactgcgatg ccatcgtcga gtccttgaga
     1981 ggtagccgac atgcgttcgc cggcagcggt gatgggaagg ctgttgggcg cctggactct
     2041 cgccacgcgt gcgtcgaacc ccagacccgc ggcgtccatg tcctggtggt gctgttcgag
     2101 cagggtcttg atctcgtcga agaccattac cgttcggagc ttcccgccct tgccgatgac
     2161 gtccagcagc caggcctctt cgccatccac gagctcgcgc cggaagcccc tcaagcgggt
     2221 cgtggccagc tccgacaagc gcaacccggt ggtgctgccg agctccagaa gtagttgcag
     2281 gcgccgccgc tccggagtgt ccggcttttc atgtagcacc tccatcgcga aggcccactg
     2341 acgctcgctc aaggaccggc gaacgtcgat gctcggcttc agcagaccca gggtgcgacc
     2401 gagattcgcc atggcgttgg ctcgcaggta ccccttctcg tgcatggcgg aaaacatgct
     2461 gcccagcacc gtgaactcgt ggcgctggct gttccgcccc aacgggcccc ggaagggcct
     2521 ccaggaggcg tcctcgcgcc tcaccttgcg ctcctgtacc caatcggcag gcggagccgc
     2581 caggaaggcc tggtaggcct gcagatcggg ctcctggagc gaggaaaggg ccttacggcg
     2641 cacgtggatg caccagaggt agaagcgctc ggcgatacgg gtgtagtccc gtcgggtctg
     2701 cccctggtac cggctcagcc actggctgat cgcctgaaca tccgtctcgg cgccccagac
     2761 gttggcctca tgggaccgga aggcgccctg tcgacccgac agggcatcgg gcagggcgcc
     2821 gtcaacgatg gggactaggg cgaacggctc cgcgttggtc accccacgac ccgccaggtc
     2881 tcgccggcga agcagggcca ggtcggtctc cggccgacgg gccgactcct tgaagggtcg
     2941 tccgccggct tctgcaatgg gggtcaacca gtcgaggaga cgccgggccc gcacgacgcc
     3001 catccgaggc acacggcggt gccaccggaa cccgtagatg ttgatgaagt tgaccaagtc
     3061 accaagcgac ttaacgccga ccttggccaa gtcgcgcggg attccggagc ccagccagaa
     3121 gccggcctcg tcctcaaggc tggcctcacg cgagatcacg cgctccagcg tcgcaagggc
     3181 ctccacgaca gcctttcgtt gcgccccaga caggcgcgag gtcgcagcga tcggcgactc
     3241 ctcgacgagg cgggcgtggg tcgtggcggc cagggcgggg gagggcaggg cagcgaagtg
     3301 tccggctttt cctgccccga actgacccct tgcggcctcc gctccgaact cctcgccgta
     3361 cagctcgagc cactccgctt ccgtgtagaa gtcctcatcg attccggcag caccgcagta
     3421 ttgggagcgc cactcctcaa gcgaagggga gggcgggggg ctgggcgccg gccgttgcat
     3481 gccttctggg tcgatgtgtc cggcttttcc tgccccccgc ttggtccgtg cgactgcgaa
     3541 gtcggggagg gcgcgcaagg ccggaagcgc tacagccagc tcggccttgc attcacgtgt
     3601 cgctcccgcg cgctcgatgg cgccgacaac caggcgcagc cggccggcga agtggcgacg
     3661 atcgctgggt ccaccggtga atgacaggta gagcctccag gcgcgttcga ggtcgactcc
     3721 atcgagcagt gcgcgcagga aggcgaagtg gtggcggcct agctcaccac agccttcgcg
     3781 cagcagacgg gtcttcgcgc ccggcgggcg gccgcgacgc ctcggtggca agccggtctc
     3841 ggccgtgagc tggcctgtgg ccggtgctac ctgtgaagcc accacctccg taggcatgaa
     3901 aaaggggtcc cccaagagcg gtaccccc
//
LOCUS       DAOORV010000264         3889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_27821_length_3889_cov_7.633803, whole genome shotgun sequence.
ACCESSION   DAOORV010000264 DAOORV010000000
VERSION     DAOORV010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27821_length_3889_cov_7.633803"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..706
                     /locus_tag="PKV56_18745"
     CDS             431..706
                     /locus_tag="PKV56_18745"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX69886.1"
                     /translation="MHFKVLDGDLATGAAVTVYKFDPGSVVPAHFHTKADEVAYVLEG
                     DFVENGATYKAGSVFAAPAGTVHGEHRTVNGAVVLFVLSRELDFIPA"
     gene            complement(981..1502)
                     /locus_tag="PKV56_18750"
     CDS             complement(981..1502)
                     /locus_tag="PKV56_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX69887.1"
                     /translation="MNMITDATHVAQHEAARQQAQHGVHAGGEYLTFRLGSEEYGIDI
                     LRVQEIRSYETPTRIANAPSFIKGVVNLRGVIVPIVDLRLKLGCDTADYDGFTVVIVL
                     NVKGRVVGAVVDSVSDVLQLSKDAVKDAPELASSMDTSFITGIGNVGDRMLILMDIES
                     FMSSADMGLISHH"
     gene            complement(1572..3212)
                     /locus_tag="PKV56_18755"
     CDS             complement(1572..3212)
                     /locus_tag="PKV56_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX69888.1"
                     /translation="MNITALMGSNSSSDPVAIGRTADRLILAATALGCAVAVYLGTLY
                     GGLSLAMGGAAAILLIAGGVYMAASGSLLSSLALSTSLMLTVALQLQIGRGTLELHFG
                     VFVSLALLLLYRDWRPILCGALVIAVHHVLVDRLQAAGFPVFCTPEADFLKVALHAGY
                     VVVQTGFELWIALTMRSGARQGIELSAMVERMDADGRISLDMSGIEVSSQAGLALKAA
                     IERLNRAMTEVQASAGNIHTSSAEIATGNQDLSSRTEQTASNLQQAASSMEQLTGTVK
                     QSADSARQANQLASSAAEVAARGGVVVSQVVSTMDEINASSQKISDIIGVIDGIAFQT
                     NILALNAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAAREIKGLIGASVEKVESGSR
                     LVANAGDTMKEIVSSVQRVSDIIGEITAAASEQSDGIGQVNSAVVQLDQMTQQNAALV
                     EQSAAAAESLRDQAGRLNEVVGTFRLDAGATQARTARPAPAAARTLPLKPSPAAKPRP
                     VASKPTPAPVVKPPQAAAAPQSAVTHAASGPSDDWETF"
     gene            complement(3449..>3889)
                     /locus_tag="PKV56_18760"
     CDS             complement(3449..>3889)
                     /locus_tag="PKV56_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX69889.1"
                     /translation="VTVRLPLTLAIMDGMSVGVGDEVYILPLASVIESFQVHEHTVKT
                     VGGTGRVVEVRGDYMPVMELERIFEVPRHNYEKSAAIMVVVEADGGRVALMVDELLGQ
                     QQVVVKNLEANYRKVEDVSGATIMGDGRVALILDVGSLVRRSRH"
BASE COUNT          728 a         1178 c         1283 g          700 t
ORIGIN      
        1 gacgtttcgc tgaacgtgct ggaagaagtg gccacgggta cgggcaagta agctgcccgc
       61 cgcctgccgg ggatccgtcc ccggcgcgcc aagcccgccc ctgccttcgc agcggcgggc
      121 tttttattgc ctgagattac tgcgcaaggc catcagtaac cttggagcac gtgcaagaag
      181 gattggtacg aaaatcgtcc tgcagtgact ggtaggaccg acttccggac tgcatcaagc
      241 gcgaaagatg acgtactccc gggagaggca tctcttggtt cgcgtggcac ggtaagtgcc
      301 attgcgtatg aggacggagt cggctctctg ccactgcgcg cgctcataaa aaccaacatg
      361 gagacaccct gtgtacacga agaacttcca cgctgattcg attgaatgac agccgagcgg
      421 gattgatggc gtgcatttca aagtgctcga tggcgacttg gcgaccggcg ccgcggtcac
      481 cgtctacaag ttcgatccgg gctccgtcgt gccggcgcat ttccacacca aggccgatga
      541 agtcgcttac gtgttggaag gggactttgt cgagaacggt gccacctaca aggcgggatc
      601 ggtatttgcc gctcctgcgg gtaccgtgca cggcgaacac cgcacggtca acggcgcggt
      661 cgtgctgttt gtcctgtcac gcgaactgga tttcatcccg gcctgatcag cgtcgacagg
      721 gaagcagcga gcttcgcgat accggcaaac cacacctggt tcgcatgatg tcgaacgggt
      781 cgccgctgcg aggctttagc cagggctgac cgcgtcgagc ggagctatca gcaccggggt
      841 gtgcgcacac ttcagatgct tggctcgccg atgaggcgaa taggcgtgtg gcgggtgggc
      901 gcatctgcgc gtgcgcaaac acgacgtgcg aaacgcgcag acaccgacgg gagtcggcaa
      961 tctgcgcgcc gcggtggcga tcagtggtgg ctgatcagac ccatgtccgc actcgacatg
     1021 aaggactcga tgtccatcag gatcagcatg cggtcgccga cgttgccgat gccggtgatg
     1081 aagctggtgt ccatcgacga agccagttcc ggagcatcct tgacggcatc cttcgacaac
     1141 tgaagaacgt cgctgaccga gtcgacaacc gcaccgacca cgcggccctt gacgttgagc
     1201 acgatgacga cggtaaagcc gtcgtagtcg gcggtatcgc atccgagctt caggcgcagg
     1261 tcgacgatcg gcacgatgac tccgcgcaga ttgacgacac ccttgatgaa cgacggcgca
     1321 ttggcaatcc gcgtcggtgt ttcgtaggaa cggatctcct gcacacgcag gatgtcgatt
     1381 ccgtactcct cggatcccag cctaaaggtc aggtattccc ccccggcgtg gaccccgtgt
     1441 tgggcttgtt ggcgcgcagc ttcgtgctgg gcgacatggg tggcatcggt gatcatgttc
     1501 atggaggttt ccttccgagc ggggtgttcg tcttcaagta ggcggtggtt gatcgtgggc
     1561 ggtgcagggg gtcaaaaggt ttcccagtca tccgacgggc cagaagccgc gtgcgtgacg
     1621 gcactttgtg gtgctgctgc agcctggggt ggcttgacga caggtgcagg agttggcttc
     1681 gaagcgactg ggcgcggctt ggccgccgga gaaggcttca gcggaagcgt tcttgccgct
     1741 gcaggcgcag gcctggcagt cctggcctgc gtcgcgcctg catccaggcg gaaagtgccg
     1801 acgacctcgt tgaggcgacc ggcctgatcc ctgagggatt cggctgcggc ggcggactgc
     1861 tcgacgagcg cggcgttctg ctgcgtcatc tgatcgagct gcacgacggc ggagttgacc
     1921 tggccgatac cgtccgactg ttcggaggct gcggcggtga tctcgccgat gatgtccgag
     1981 acccgctgca cggagctgac gatctccttc atcgtgtcgc cagcattggc caccaggcgc
     2041 gagcccgact cgaccttctc gaccgatgcg ccgatcagcc ccttgatctc gcgggcggcc
     2101 tcggccgaac ggccggccag actgcgcacc tcggaggcga cgacggcaaa gccacgacct
     2161 tgctcgccag cgcgggcggc ctccacagcg gcattgagcg cgaggatgtt ggtctggaag
     2221 gcgatgccat cgatgacgcc gatgatgtcg gagatcttct gcgaggaggc gttgatctcg
     2281 tccatggtgg agaccacctg ggagaccacg acgccaccgc gtgcggcgac ctcggcggca
     2341 gacgaagcga gctggttggc ctggcgggcg gagtcagccg actgcttgac cgtgccggtg
     2401 agctgctcca tcgaggaggc ggcttgctgc aggttggagg ccgtctgctc ggtgcggctg
     2461 ctcaggtcct ggttgcccgt cgcaatctcg gcgctggagg tatggatgtt gcccgcagaa
     2521 gcctgcactt ccgtcatggc ccggttcaat cgttcaatgg cggccttcaa cgccaggccc
     2581 gcctgcgatg acacttcgat gccgctcatg tccagagaga tccggccatc ggcatccatc
     2641 cgttccacca tggcgctgag ttcgatgccc tggcgggcgc cgctgcgcat ggtgagggcg
     2701 atccagagct cgaagccggt ctgcacgacc acgtagccgg catgcagggc caccttcagg
     2761 aagtcggctt cgggagtgca gaacaccggg aagcccgccg cctgaaggcg atcaaccaga
     2821 acgtggtgga cggcaatgac cagagcaccg cacaggatgg gtcgccagtc gcggtacagc
     2881 agcagcagtg cgagagagac gaacacaccg aaatggagtt caagcgtccc acggccgatc
     2941 tgcagctgaa gggccaccgt cagcatcagg ctggtggaca acgcgaggct cgacagcagg
     3001 ctcccgctgg ccgccatgta gacgccgccg gcgatcagca ggatggccgc tgcgccaccc
     3061 atcgcgagcg aaagcccgcc gtacaacgtg ccgaggtaga ccgcgaccgc gcagcccagt
     3121 gcagtggccg cgaggatcag tcgatcggcg gtgcgtccga tcgcgacggg gtccgacgac
     3181 gagttcgaac ccatcaaggc ggtgatattc atgatgcaag aagtcccttc gtgatttgca
     3241 agtgactgag gcaggcgacg cgcggcagcc aatcagaaac ttcaagtggt tatcggaaca
     3301 atctgaacta tttgaaggtt tctgtcgctg aacttcggct gctgcggcgg gtgcgccgca
     3361 ccagaaggaa cgccccgacc gacgggattc cgtgcatgtc ggggcgctgg agatgcagcc
     3421 gaaggatcga ctgtcggatt cctgcagatc agtgcctgga acggcgcacc aggcttccta
     3481 catcgaggat cagtgcgacc cggccatcac ccatgatggt ggcgccggag acgtcctcga
     3541 ccttgcggta gttggcctcg aggttcttga ccacgacctg ctgctggccg agcagttcat
     3601 cgaccatcag ggcgacacga ccgccatcgg cttcgacgac gaccatgatg gcagccgact
     3661 tttcgtagtt gtgacgtggc acctcgaaga tgcgttccag ttccatcacc ggcatgtagt
     3721 cgccacgcac ctcgaccacg cggccggtgc cgcccacggt cttgacggtg tgctcatgca
     3781 cctggaacga ctcgatgacc gaagccagcg gaaggatgta gacctcatcg ccgacgccga
     3841 cgctcatgcc gtccatgatg gccagcgtca gcggcaggcg caccgtgac
//
LOCUS       DAOORV010000265         3874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_27968_length_3874_cov_5.979576, whole genome shotgun sequence.
ACCESSION   DAOORV010000265 DAOORV010000000
VERSION     DAOORV010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27968_length_3874_cov_5.979576"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PKV56_18765"
     CDS             <1..867
                     /locus_tag="PKV56_18765"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOX69890.1"
                     /translation="LDKLLGAFLNPEGSPGILVNSQRPIGMVHLTCAHELGHYFLGHE
                     TTTDEHIEYGEGASPSERAANEFAYHLLMPRWLVVHVMKQKGWSLTDLPRPEVVYQLS
                     LRLGVSFRAMVWSLRSIKFLTLPLATQIAAHSPKALKEAVLPDRRAPAEGGDVWVLDP
                     RDQAFIIEPRAADRFILELPNHAGAGYVWTAEEASSAGFAIKPVVVDATRHDLESGPV
                     VVGSSATLSYELEPSAAATSTDAIQPLGLTETQPWNRGAPRDHVGLRAHYETLVEGLS
                     RGTRQRRLQEAR"
     gene            956..1510
                     /locus_tag="PKV56_18770"
     CDS             956..1510
                     /locus_tag="PKV56_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOX69891.1"
                     /translation="MSDLNRDAAAAPAALSAEFLYQTAGALTPGWRPGQGLRTVEAIR
                     ATQAPGQPLDAHFPYQAVDPISTDHPVAPAGQPLYASAFALRSPAMQPIIDALTRGHA
                     VGLVTRVTLGLFSPVGGVVRNEVNVLPGQYHALLAVGWGTAPSSGMRHLLVRNSWSEM
                     WGLSGHAWLPEPFVNLHVVEVFGR"
     gene            1513..2421
                     /locus_tag="PKV56_18775"
     CDS             1513..2421
                     /locus_tag="PKV56_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69892.1"
                     /translation="MGMVISKGRIIEAWLAAIDHLEQNGGRYRNLMLEINAADEMTFA
                     DRRVISAVDEALRTRGETTVLTVAGTIFPQGLYKRVGAADLAAHFLGIMASAKKRGTW
                     GTYAMRLMSRPGREWNTVINPLAILIGKLQRAASPNGKAIVSNYELGVADANDLASDE
                     PVFCDVPLYSPAHDATMVRNQPCLMHLSFKLVDKTTLELTAIYRSHYYAQRALGNFIG
                     LRHLMNFVAAQARVQAGRLTCISTDASLDLASWGSAAESRAALQRIRAVATTVDSAPA
                     SAGTARAMTPAPHLVSGESSATHTGA"
     gene            complement(2354..3028)
                     /locus_tag="PKV56_18780"
     CDS             complement(2354..3028)
                     /locus_tag="PKV56_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOX69893.1"
                     /translation="MTPSSFVQALAAIQLPNVFNPYADTCPVHDRKDAAAVRRRSLRT
                     LLAASQSLGIDTIWMGRDLGYRGGRRTGLALTDEMRLTQFPTIYPGAEAAKSTRSPAV
                     AERTAAEIWAVLAQLDLPPLLWNVFPFHPHEPGEPFTNRKFTATELKFADALNRQLLN
                     WLGIRRIVAIGQDAAGYAAQFGVKVECVRHPSYGGVTEFREGIRRLYGLPKIRRLQDE
                     VQGSLL"
     gene            complement(3025..>3874)
                     /locus_tag="PKV56_18785"
     CDS             complement(3025..>3874)
                     /locus_tag="PKV56_18785"
                     /inference="COORDINATES: protein motif:HMM:NF037400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69894.1"
                     /translation="VHLSGLTGQREELLRSLASSCGALTAKLQAEFCTWGEDNVAAAL
                     GELMAQLALVARCFGLGLAAVARENMAKIKDRWPGDNPIFWQLPDAVNCEPHERFDRQ
                     FSIQFTERRVGARLVVVQQLCSVNIGDPLTDNSHTDDGYRFHDVFHLSYVAHLGWSPV
                     IRALLKLKRKSQPKTDENEDGARAIIIEEGIATWIFNHAKSRGDFFAGVTEDRLDFSL
                     LKQVRSMVQGFEVADCPSWLWARAIVRGFEVFRELREHRHGTVAVDMEARTLTFTPGT
                     AQGNLS"
BASE COUNT          786 a         1177 c         1195 g          716 t
ORIGIN      
        1 ctagacaagc tgctcggggc cttcctgaat cctgaaggtt caccgggaat tcttgtgaat
       61 tcccagcgac cgatcggcat ggttcatctg acgtgcgcac acgagttggg acactatttc
      121 ctcggacatg aaaccacgac cgatgagcac atcgaatacg gcgaaggcgc atccccgagc
      181 gagcgtgcgg ccaatgagtt cgcgtaccac ctgctgatgc cgcgttggct ggtcgtgcac
      241 gtcatgaagc agaagggatg gagtcttact gacctgccac gcccggaagt ggtgtaccaa
      301 ctgtcgctgc gactcggagt gagcttccga gcgatggtct ggtcgctgag aagcatcaag
      361 ttcttgacgc tgcctctggc gacacaaatc gcagcgcact ccccaaaggc attgaaagag
      421 gccgtcctac cagatcgcag agcacctgcc gaaggtggtg acgtatgggt gctcgatccg
      481 cgggatcaag cgttcatcat tgaacctcga gccgccgatc gcttcatctt ggagctgccc
      541 aaccacgccg gcgcgggata tgtctggacg gcagaagagg cctccagcgc tgggttcgcg
      601 atcaagccag tagtagtcga cgcgacccgg cacgacctgg agagcggccc ggttgtcgtc
      661 ggtagcagcg caacgttgtc ctacgaactg gagcccagtg ccgccgcgac ttcgacggac
      721 gcaatccagc ctctcggcct gacggagaca caaccctgga accgaggagc gccaagagac
      781 cacgtagggc tgcgcgctca ctacgagact cttgtcgaag ggttgagccg gggcacgcgc
      841 cagcgacgac tgcaggaggc gcgctgacgt gcaggtacag ccagcagtcg acttctcggc
      901 ctcgtttggt ccggctcgaa atcaagggca acgcagcacg tgcatggcgt ttgcgatgtc
      961 cgatctcaac cgcgatgccg ctgcagcgcc tgcagcactc agtgcagagt tcctgtacca
     1021 aaccgccggg gcgcttacac ccggttggcg accaggccag ggcttgcgca cagtggaagc
     1081 cattcgtgcc acacaggccc ctggccagcc actcgatgcc cactttcctt accaggcggt
     1141 ggacccgatc agtaccgatc atcctgtagc acctgctggc cagcccctgt atgcaagcgc
     1201 attcgctctc cgctcgcccg cgatgcagcc catcatcgac gcactgaccc gcggccacgc
     1261 agttggactg gtcactcgcg tgacgcttgg tttgttctcg ccggttggcg gcgtcgtgcg
     1321 gaacgaggtc aatgtgctgc ctgggcagta ccacgcactg cttgcggtag gttgggggac
     1381 ggccccgagt tccggaatgc ggcacctgct ggtgcgcaac agctggagtg aaatgtgggg
     1441 actgagcggc catgcctggc tgccagagcc gtttgtgaac ctgcatgtcg ttgaggtctt
     1501 tgggagatag cgatgggtat ggtcatcagc aaggggcgca tcatcgaggc atggctcgcg
     1561 gccatcgatc accttgagca gaatggcggt cggtaccgaa atctcatgct ggagatcaat
     1621 gcggcagacg aaatgacatt tgccgacagg cgtgtcatct cagccgtcga cgaggcattg
     1681 cggacccgcg gcgaaaccac cgtgctgacc gtcgctggga cgatcttccc gcaaggcctt
     1741 tacaagcggg taggcgcggc cgatctggcc gcccactttc tcggcatcat ggcgagcgcc
     1801 aagaagcgtg gaacttgggg cacgtacgca atgcgtttga tgtcgcggcc tggacgcgaa
     1861 tggaacacag tcatcaaccc attggcgata ctgattggaa aactgcaacg cgccgcaagc
     1921 ccgaacggca aggcaattgt ctccaactac gaactaggag tagcggatgc caatgacctg
     1981 gctagcgatg agccagtgtt ctgcgacgtg ccgctttaca gcccagcaca tgacgcgacc
     2041 atggtgcgca atcaaccttg cttgatgcac ttgagcttca aactggtcga caagacaacc
     2101 ctcgaattga cagcgatcta tcgctcgcac tactacgcgc aacgcgcctt gggcaacttc
     2161 atcggcctgc gccatttgat gaacttcgtg gcagcacaag ctcgagttca agcgggccga
     2221 ctgacctgca tctcgacaga cgcgagcctg gatctagcaa gttggggctc ggcagcagag
     2281 agtcgggccg cactgcagag aattcgtgcg gtcgcgacaa cggtcgattc ggcaccagcc
     2341 agcgcaggca cggctagagc aatgacccct gcacctcatc ttgtaagcgg cgaatcttcg
     2401 gcaacccata caggcgcctg atcccctccc gaaattcggt gacgccgcca tagctgggat
     2461 gccgcacgca ctccactttg acaccgaatt gagctgcgta gccggcagcg tcctggccga
     2521 tggcgacgat gcgacggatc ccgagccagt tgagcagttg gcggttgaga gcgtcggcaa
     2581 acttcagttc cgtcgccgtg aacttgcgat ttgtgaaggg ctcgcctggc tcgtgcggat
     2641 gaaacgggaa cacattccac aacaagggcg gcaggtcgag ttgcgcgagc accgcccaga
     2701 tctccgcggc agtgcgctcg gcgacggcag ggctgcgagt tgacttggca gcttcggcac
     2761 cagggtagat cgttggaaac tgcgtcagac gcatttcgtc ggtcagtgcc aaaccggtgc
     2821 ggcggccgcc gcggtatccg aggtcccgcc ccatccagat ggtgtcgatc cctagcgact
     2881 gcgaggcggc aaggagggta cgcaagctgc ggcggcgcac agcagcggca tctttcctgt
     2941 catggaccgg gcaagtgtcg gcgtaaggat tgaacacgtt cgggagttgg atggccgcga
     3001 gcgcctggac gaagctggaa ggggtcacga taggttgcct tgcgctgtgc cgggagtgaa
     3061 tgtcaacgtg cgcgcttcca tgtctactgc gaccgtgcca tgcctatgct cgcgaagttc
     3121 acgaaagacc tcgaagcctc gcacgatggc tcgcgcccac aaccatgatg gacagtcggc
     3181 cacctcgaat ccctgaacca tgctgcgtac ctgcttcagc agcgagaagt cgaggcgatc
     3241 ctcggtaacg cccgcaaaaa agtctcctcg ggatttcgcg tggttgaaga tccaggtagc
     3301 tatgccttct tcgatgatga tggcgcgggc accatcttcg ttctcatcgg tcttcggctg
     3361 cgacttgcgc ttgagtttga gcaaagcgcg gatcacgggc gaccagccaa ggtgggcgac
     3421 ataggagaga tgaaacacgt cgtggaagcg gtagccgtca tcggtatggc tgttgtcggt
     3481 cagtgggtcc ccgatgttga cgctgcaaag ctgctgcaca acaaccaggc gggccccgac
     3541 ccttcgttcg gtgaactgaa tgctgaactg ccgatcgaac cgctcatgcg gctcgcagtt
     3601 gacggcgtcc ggtagctgcc aaaagatggg attgtcgccc ggccaccgat ctttgatctt
     3661 ggccatgttc tcgcgcgcca ccgcggctag ccccaacccg aagcagcgcg cgactagcgc
     3721 aagttgcgcc atcaactcac ccaacgctgc cgccacgttg tcctcacccc aggtgcagaa
     3781 ctctgcttga agcttcgcag tcagcgcgcc gcaggacgaa gcaagcgatc gcagcagctc
     3841 ttcacgctgc ccggtcaggc ccgacaggtg cacg
//
LOCUS       DAOORV010000266         3825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_28466_length_3825_cov_9.387798, whole genome shotgun sequence.
ACCESSION   DAOORV010000266 DAOORV010000000
VERSION     DAOORV010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28466_length_3825_cov_9.387798"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKV56_18790"
     CDS             complement(<1..253)
                     /locus_tag="PKV56_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX69895.1"
                     /translation="MQPLVLTLKGFRGIRDGLGRDELTLDLERLTQDAQLIAIVGANG
                     SGKSTIMDCLVPYIGLPSRAALAGAGGFSYYDHVYLPESV"
     gene            complement(338..2023)
                     /gene="merA"
                     /locus_tag="PKV56_18795"
     CDS             complement(338..2023)
                     /gene="merA"
                     /locus_tag="PKV56_18795"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HOX69896.1"
                     /translation="MTELVIGGMTCDSCATHVRQALEKVPGVHAAQVSYPKGRARLTL
                     APDTSMTALVAAVTALGYRARVADGNADRPRDGLLDKARSWLGGGQKATGGDEALHVA
                     VIGSGGAAMAAALKAAERGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHVAHLRRES
                     PFDGGIAASTPVIDRGRLLAQQRSRVDELRHAKYESILASTLAITVLRGEARFKDRRH
                     LVVRLHEGGERELAFDRCLIATGAHPSIPPILGLKDTPYWTSTEALDSDAIPPRLAVI
                     GSSVVALELAQAFARLGSQVTVLARHTLFYREDPAIGEAVTAAFRAEGITVLEHTQAS
                     RVDHTDGEFVLATDRGELRADRLLVATGRAPHTQGLGLDAAGVTVDAQGAIVIDAAMR
                     SSASHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGEAALDLSAMPAVVFTDPQVATV
                     GLTETEARAKGLEVDTRTLPLDNVPRALANFDTRGFIKLVAEAGGGRLLGVQAVTPDA
                     GELIQSAALALRARMTVHDLADQLFPYLTMVEGLKLCAQTFTKDVKQLSCCAG"
     gene            complement(2058..2465)
                     /gene="merC"
                     /locus_tag="PKV56_18800"
     CDS             complement(2058..2465)
                     /gene="merC"
                     /locus_tag="PKV56_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HOX69897.1"
                     /translation="MNVMTRIADKAGALGSVVSAMGCGACFPALASLGAALGLGFLSQ
                     FEALFVTVLLPLFAGLALLANALGWFSHRQWHRSVLGMVGPAIVLVASFSVAEKLLYV
                     GLATMVAVSVWDFVSPANRRCGPDGCEVPVRQS"
     gene            complement(2492..2767)
                     /gene="merP"
                     /locus_tag="PKV56_18805"
     CDS             complement(2492..2767)
                     /gene="merP"
                     /locus_tag="PKV56_18805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HOX69898.1"
                     /translation="MKKLFLIALFAASAGAVHAAPQTVTLDVPGMTCAACPITVKKAI
                     SKVDGVSKVDVSYEKRQAVVTFDDAKASVQKLTQATENAGYPSTVKR"
     gene            complement(2781..3131)
                     /gene="merT"
                     /locus_tag="PKV56_18810"
     CDS             complement(2781..3131)
                     /gene="merT"
                     /locus_tag="PKV56_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HOX69899.1"
                     /translation="MTESQNGRGALAAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TALEPYRPIFIGAALVAMLFAWRRIYRPAQACKPGEVCAIPQVRSTYKVVFWIVGALV
                     LIALAFPYVLPLFY"
     gene            3203..3610
                     /gene="merR"
                     /locus_tag="PKV56_18815"
     CDS             3203..3610
                     /gene="merR"
                     /locus_tag="PKV56_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HOX69900.1"
                     /translation="MEREQNGLTIGAVAKAAGVNVETVRFYQRRALLAEPDKPYGGIR
                     RYGADDVARVRFVKAAQRLGFSLDEIAGLLQLDDGTHCDEARELAEAKLADVRKKLKD
                     LRRIESALAGLVRDCCMSRGKVSCPLIDTLQKQ"
BASE COUNT          631 a         1271 c         1320 g          603 t
ORIGIN      
        1 tcacgctttc gggcaggtac acgtggtcgt agtaggagaa gccgcccgca ccggcaagcg
       61 cggcgcggct cggcagtccg atgtagggca cgaggcaatc catgattgtc gacttgccgc
      121 tgccgttcgc cccgacgatg gcgatcagct gcgcgtcctg ggtcagacgc tcgaggtcga
      181 gcgtgagctc gtcgcggccc aggccgtcac gaatgccgcg gaatcccttg agggtgagta
      241 cgagaggttg catggagagc ctcgaggaag cagatgcctc aggctcccat gaacagtctt
      301 cacatcaagc cagtcgtccg cactcaaggc acgcatgtca gccggcacag cacgagagct
      361 gcttgacgtc cttggtgaag gtctgcgcgc acagcttgag cccttccacc atggtcagat
      421 acgggaacag ctgatcggcc aggtcatgca cagtcatgcg cgctcgcagc gcgagcgcag
      481 cgctctggat caactcaccc gcatcgggtg tgacggcctg cacgcccaac agccgcccgc
      541 cgcccgcctc ggcaacgagc ttgatgaagc cccgggtgtc gaagttggcc agcgcgcgcg
      601 gcacgttgtc caggggcagc gtgcgcgtgt cgacttcgag gcccttggcc cgcgcctcgg
      661 tttcggtcaa gccgacagtg gccacctgcg ggtccgtgaa gaccacggct ggcatcgcgc
      721 tcaggtcgag cgcagcctct cccccggtca tgttgatcgc cgcgcgcgtg ccggcggccg
      781 cggccacgta gacgaactgc ggctggtcgg tgcagtcgcc ggcggcgtag atgtgcgacg
      841 cgctggagcg catcgccgcg tcgatgacga tggcgccctg cgcgtcgacg gtcacaccgg
      901 ccgcgtcgag cccgaggccc tgcgtatggg gtgcgcggcc cgtggcgacg agcagccggt
      961 cggcccgcag ctcgccgcga tcggtcgcga gcacgaactc gccgtcggtg tggtccaccc
     1021 ggctggcttg cgtgtgttca agcaccgtga tgccttcggc gcggaaggcc gctgtcacgg
     1081 cctcgccgat cgcggggtct tcgcggtaga acagcgtgtg gcgcgcgagc acggtcacct
     1141 ggctgcccag ccgcgcgaag gcctgcgcga gctccagcgc caccaccgac gagccgatca
     1201 ccgcgagacg tggcgggatc gcgtcgctgt ccagtgcttc ggtggatgtc cagtacggcg
     1261 tgtccttcag cccgaggatc ggcgggatcg acggatgtgc gccagtggcg atcaggcaac
     1321 ggtcgaaggc cagttcgcgc tcaccgcctt catggagccg gaccacgaga tgccgtctgt
     1381 ccttgaagcg cgcctcgccg cgcagcaccg tgatcgccag ggtgcttgcg aggatgctct
     1441 cgtacttcgc atgccgtagt tcgtcgacgc gggagcgctg ctgcgcaagc aggcgaccgc
     1501 ggtcgatgac aggcgtgctc gcggcgatcc cgccgtcgaa gggactctcg cgccgcaggt
     1561 gcgccacgtg cgccgcgcgg atcatgatct tcgacggcac gcagcccacg ttgacgcagg
     1621 taccgccgat cgtgccgcgc tcgatgagcg ttacgcgcgc gccccgttcg gccgctttca
     1681 gcgccgcggc catcgcggcg ccgccgctgc cgatgacggc gacgtgcaac gcctcgtccc
     1741 cgccggtcgc cttctggcca ccgccgagcc agctgcgcgc cttgtcgagc aggccgtcgc
     1801 gcggccggtc ggcattccca tctgcgacac gggctcgata gcccagcgcg gtcaccgccg
     1861 ccacgagcgc cgtcatggac gtgtcggggg cgagcgtgag cctggccctg cccttggggt
     1921 aggacacctg cgcggcgtgc acgccgggaa ccttctccaa ggcctggcga acatgcgtgg
     1981 cgcacgagtc gcaggtcatg ccgccaatca cgagttcggt catgggttgc ttttcgagag
     2041 gagctggcgt tacgccttca ggattgcctg accgggactt cgcagccgtc agggccgcag
     2101 cgccggtttg caggggagac gaagtcccac acggataccg cgaccatcgt cgcgaggccc
     2161 acgtagagca gcttctcggc cacgctgaag ctggcgacca gcacgatggc cggaccgacc
     2221 atgccgagca cgctgcggtg ccattggcga tgactgaacc agcccagcgc attggccagc
     2281 agcgcgagcc ccgcgaacag cggcagcagc acggtgacga acaaggcctc gaactggctc
     2341 aggaagccga gcccgagggc ggcgccgagg ctggccaggg ccgggaagca ggcgccgcag
     2401 cccatcgccg agacgacgct gcccagcgca ccggccttat cggcaatgcg cgtcatcacg
     2461 ttcatggtgg cctcctcgcc gctgctgcgg ttcagcgctt gacggtggac gggtagcccg
     2521 cgttctcggt cgcctgggtc agcttctgca cgctggcctt ggcatcgtcg aaggtcacga
     2581 cggcctggcg cttctcgtag ctcacgtcga ccttgctcac gccgtcgacc ttcgagatcg
     2641 ccttcttcac ggtgatcgga caggctgcgc aagtcatgcc gggcacgtcg agcgtgacgg
     2701 tctggggcgc tgcatggacg gcgccggcgg atgcggcgaa caaggcgatc aggaacagct
     2761 tcttcatggc gaactccggg tcaatagaac aagggaagga catacgggaa ggcgagcgcg
     2821 atcagcacca gcgcgccgac gatccagaag acgaccttgt aggtggaccg cacctgcggg
     2881 atcgcgcaca cctcgcccgg tttgcaggcc tgtgccggtc gatagatgcg gcgccaggcg
     2941 aagagcattg caaccagcgc ggcgccgatg aagatcgggc gatagggctc cagcgcggtg
     3001 aggttgccga tccatgcgcc gctgaagccg agagccacca gcaccagggg gcccaggcag
     3061 caggtggacg ccaggatcgc ggccagtcct ccggctgcga gggctccgcg cccgttctgc
     3121 gattcggtca tgcgctttcc ttggcgaatc tgaagacgat ggcgtaagct tatttccgta
     3181 ctcatgtacg gagtcaagcg acatggagcg cgagcagaac ggcctgacga tcggggccgt
     3241 cgccaaggcg gccggggtca acgtggagac ggtgcgcttc tatcagcgcc gggccctgct
     3301 ggccgagcca gacaagccct acggcggcat tcggcgctat ggcgcggatg acgttgcgcg
     3361 cgtgcggttc gtgaaggccg cgcagcgcct ggggttcagc ctcgacgaga tcgcagggtt
     3421 gttgcagctc gatgacggca cgcactgcga cgaggcgcga gaactcgctg aagccaagct
     3481 cgcggacgtg cgcaagaagc tgaaagacct gcggcgcatc gaatcggcgc tggccggcct
     3541 cgtccgcgat tgctgcatga gccgcggcaa ggtgtcgtgt ccgttgatcg ataccttgca
     3601 gaagcagtga gcagcgcctc ggtcagttcg ggccactgtt gacaccgcgg actcaacata
     3661 agggatgcag gttgtccatc accgtggtct tcccgcgccc gttgccgccc gcaatggcca
     3721 ccagttgcgc gtcaccgacc agcgcctcga gatcgagcgt gatcgtgtcc cgaccgagac
     3781 cgctgcggat ccccttgaaa ccagtgagcg tcagcgacag cggtt
//
LOCUS       DAOORV010000267         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_28526_length_3819_cov_9.391605, whole genome shotgun sequence.
ACCESSION   DAOORV010000267 DAOORV010000000
VERSION     DAOORV010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28526_length_3819_cov_9.391605"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKV56_18820"
     CDS             complement(<1..240)
                     /locus_tag="PKV56_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX69901.1"
                     /translation="MVDSAVRLLSYAFFAIALMFAAVAWTLDDVFFFRLATEALIFGG
                     LALSVDLLLGRTGLLPLGQALFFGFGAYVSALVLKH"
     gene            complement(233..1117)
                     /locus_tag="PKV56_18825"
     CDS             complement(233..1117)
                     /locus_tag="PKV56_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX69902.1"
                     /translation="MLEWLQFVLAPQVINGLSIGVAVVLMALGLTIIFGLLDVINMAH
                     GEFYAIGAYAALALIAVGLPFWWALVLTPLLMAVVGYATERVLIQRVFHSKDRHTLTL
                     LLTFGIAIILEDLLKIVFGANPLRIEQPITGATEMFGLFFPNYRLFLMAVGALVIGAV
                     WIVVFRTRLGAMVRAAAFDRHMAASLGVPVSVVYAGTFAFGVALAGLSGVLLAPIYSV
                     FPTMGRDFVLIAFSVVIIGGMGSIKGAVIAGLLLTQVQSISSLFISPVWSDPLLFSIM
                     VAVLMWRPHGLFGRLGHG"
     gene            complement(1128..2294)
                     /locus_tag="PKV56_18830"
     CDS             complement(1128..2294)
                     /locus_tag="PKV56_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX69903.1"
                     /translation="MKKTFVATTLALAATATFAQAGAPIKIGVVTPLSGTYAGIGQQV
                     KWGLDLAAAQINASGGVMGRKLELLYEDEEANPAVATQKAEKLFQVNKVDFLTGTVNS
                     GSTLAVGQVAERNNRLIATTVSFADSITADKCSPNVFRVNARAGMQSAALADWLATTQ
                     KDASVFYLGPDYEMGRSTVAAFKSAAEGKGAKTAGEVFAPLDNKDYSPYFGQIRGAKP
                     TVIYTSVAGNDTVRLFTQMAEFGVNRNIQVVGASGTVTGQNLAAIGKAADGFVTGVGY
                     STSIPSAENQKFVADFTAANKAAPDLYGADSYGVLFAYKAAVEKAKSTETDAVRTALR
                     GLQWNTPQGVKTLRAGDHQAMQDMYAVKVNGGKFEIVGKVAAAAAIGADTCSKF"
     gene            complement(2330..3142)
                     /locus_tag="PKV56_18835"
     CDS             complement(2330..3142)
                     /locus_tag="PKV56_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOX69904.1"
                     /translation="MNLPELNHPAHTLPAVPDGFRVEIDAARERADIVLDRPPFNVVS
                     MLAREQLRIAFEALDADERVRVIVVRGQGEHFSSGGDIKGFLAATPEKVSQLAWNIAA
                     PARCSKPVIAAGRGYCFGVGFELSLACDFRLVTETTLYALPEQKLGQIPGSGGSARLQ
                     KMVGITRTKDIVMRSRRIPGRQAFDWGVATDCVPDAELEQATDALVDELRSFSPLAQR
                     TAKKLLNDTEDSPLSIAIELEGHCYSRLRSSEDFREGVEAFHSKRKPNFIGR"
     gene            complement(3139..>3819)
                     /locus_tag="PKV56_18840"
     CDS             complement(3139..>3819)
                     /locus_tag="PKV56_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX69905.1"
                     /translation="LFVNHYGSSEVYTFSINQRAVAKPGSAGRAGLNTRLRVVKLDST
                     DPGQFAAAGEEGQIVADLLGDEAFEGYHRRPDANAKSLHGGWYFTGDMGYVDAEGDLY
                     VTGRVDDMIISGGENISPVDIESVLSLHPAVDEVAVAGLPDARWGQKVVAFVKLHANA
                     DAEPDAATLDAHCRSSDLVNFKRPRDYVFVREIPKSPVGKVLRRKLQAGEYEVLSPSH
                     GSANQGTP"
BASE COUNT          618 a         1255 c         1308 g          638 t
ORIGIN      
        1 gtgcttgagc accagcgccg agacgtacgc cccgaagccg aagaacagcg cctgccccag
       61 cggcagcagg ccggtgcggc ccagcagcag gtcgacactg agtgccaggc ctccgaagat
      121 caaggcctcg gtcgccaggc ggaagaagaa cacgtcgtcg agcgtccagg ccaccgccgc
      181 aaacatcagc gcgatggcga agaaggcata cgacagcagt cgcaccgcgg agtcaaccat
      241 gaccgagcct cccgaagagg ccgtgcgggc gccacatcag caccgcgacc atgatggaaa
      301 acagcagcgg atcgctccac accggcgata tgaacaggct gctgatggac tgcacctgcg
      361 tcagcagcag gccggcgatg acggcgccct tgatcgagcc catgccgccg atgatgacaa
      421 cgctgaaggc gatcaacacg aagtcccgtc ccatcgtcgg gaagaccgag tagatcggcg
      481 ccagcagcac acccgacagc cctgccagcg ccacgccgaa ggcgaaggtg ccggcgtaga
      541 ccacgctcac cggcacgccc agcgacgccg ccatgtggcg gtcgaaggcg gcggcgcgca
      601 ccatcgcccc cagccgcgtg cggaagacga cgatccacac cgcgccaatc accagcgcgc
      661 ccactgccat caggaacagc cggtagttcg ggaagaacag gccgaacatt tcggtggcgc
      721 cggtgatggg ctgctcgata cgcagcgggt tggcaccgaa cacgatcttc agcaggtctt
      781 ccaggatgat ggcaatgccg aaggtcagca ggagtgtgag cgtgtggcgg tctttgctgt
      841 ggaaaacgcg ctgtatcaac actcgctccg tcgcatagcc caccaccgcc atcaacagcg
      901 gcgtgagcac cagtgcccac cagaagggca ggcccacggc tatcaacgcc agcgccgcat
      961 aggcgccgat ggcatagaac tcgccgtgag ccatgttgat gacgtcgagc agaccgaaga
     1021 tgatggtcag ccccagcgcc atcagcacca ccgcgacgcc gatcgacagg ccgttgatga
     1081 cctgcggcgc caacacgaac tgcagccatt ccagcatcgc cgtggcctca gaatttgctg
     1141 caggtgtcgg cgccgatggc ggcggctgcc gcgaccttgc cgacgatctc gaacttgccg
     1201 ccgttcacct tcacggcata catgtcctgc atggcctggt ggtcgccggc gcgcagcgtc
     1261 ttcacgcctt gcggggtgtt ccattgcagg ccacgcaacg ccgtgcgcac agcgtcggtc
     1321 tcggtgctct tggccttttc caccgcggcc ttgtaggcga agagcacgcc gtagctgtcg
     1381 gcgccgtaca ggtcgggcgc ggccttgttg gcggcggtga agtcggcgac gaacttctgg
     1441 ttctcggccg agggaatgct ggtggagtag cccacgccgg tgacgaagcc gtcggccgcc
     1501 ttgccgatgg ccgccaggtt ctggccggtg accgtgcccg aggcgcccac cacctggatg
     1561 ttccggttga cgccgaactc ggccatctgc gtgaacaggc gcaccgtgtc gttgccggcc
     1621 accgaggtgt agatcacggt cggcttcgcg ccgcggatct gcccgaagta gggcgagtag
     1681 tccttgttgt ccagcggcgc gaacacctca ccggcggtct tcgccccctt gccttcggcc
     1741 gcgctcttga acgcggccac ggtgctgcgg cccatctcgt agtcgggccc caggtagaag
     1801 acgctggcgt ccttctgcgt cgtggccagc cagtcggcca gcgccgcact ctgcatgccg
     1861 gcacgggcat tgacgcggaa gacgttgggg ctgcacttgt cggcggtgat gctgtcggcg
     1921 aaggacaccg tggtcgcgat cagccggttg ttgcgctcgg ccacctggcc caccgccagc
     1981 gtgctgccgc tgttgacggt gccggtgagg aagtcgacct tgttcacctg gaacagcttc
     2041 tcggccttct gcgtggccac cgcggggttg gcctcttcgt cctcgtagag cagttcgagc
     2101 ttgcggccca tcacaccgcc gctggcgttg atctgcgccg cagcgagatc caggccccac
     2161 ttcacctgct ggccgatgcc tgcataggtg cccgacagcg gcgtgacgac gccgatcttg
     2221 atgggcgcgc cagcttgggc aaacgtggcg gtggcagcca gcgccagcgt ggtggcaaca
     2281 aaggtcttct tcatgtctgt ctccgtgctt gtggtggggg gtgagggaat cagcggccga
     2341 tgaagttcgg cttgcgcttg ctgtggaagg cctccacgcc ttcgcggaag tcttcgctgc
     2401 tgcgcaggcg cgaatagcag tggccttcga gttcgatcgc aatcgacagc ggcgagtcct
     2461 cggtgtcgtt gagcagcttc ttggccgtgc gctgggccag cgggctgaag ctgcgcagct
     2521 cgtcgaccag cgcatcggtg gcctgttcga gttcggcgtc gggcacgcag tcggtggcca
     2581 cgccccagtc gaaggcctgg cggccgggga tgcggcggct gcgcatcacg atgtccttgg
     2641 tgcgtgtgat gcccaccatc ttctgcaggc gcgcgctgcc gccgctgccc gggatctggc
     2701 ccagcttctg ctcgggcagc gcgtacagcg tggtctcggt gacgaggcgg aaatcgcagg
     2761 ccaggctgag ctcgaagccg acgccgaagc agtagccgcg gccggccgcg atcaccggct
     2821 tgctgcagcg tgccggcgcg gcgatgttcc aggccagctg gctgaccttc tcgggcgtgg
     2881 cggccaggaa gcctttgatg tcgccgccgc tgctgaagtg ttcaccctgg ccgcgcacga
     2941 cgatcacgcg cacccgttcg tcggcgtcca gcgcctcgaa ggcgatgcgc agctgttcac
     3001 gcgccagcat gctgacgaca ttgaacggcg ggcggtcgag cacgatgtcg gcacgttcgc
     3061 gggcggcgtc gatctcgacg cggaagccgt cggggacggc gggcagcgtg tgcgcggggt
     3121 ggttcagttc gggcaggttc atggtgttcc ttggttggca gagccgtgac tgggggacag
     3181 gacttcgtac tcgccggcct gcagctttcg ccgcagcacc ttgccgacgg ggctcttggg
     3241 gatctcgcgc acgaagacgt agtcgcgcgg gcgcttgaag ttgacgaggt cgctgctgcg
     3301 gcagtgcgcg tccagcgtgg cggcgtcggg ctcggcatcg gcgttcgcgt gcagcttcac
     3361 gaaggccacc accttctggc cccagcgcgc atcgggcagg ccggccacgg ccacctcatc
     3421 gacggccggg tgcagcgaga gcaccgactc gatgtcgacc ggcgagatgt tctcgccgcc
     3481 gctgatgatc atgtcgtcga cacggccggt gacgtagagg tcgccctcgg cgtcgacata
     3541 gcccatgtcg ccggtgaagt accagccgcc gtgcaggctc ttggcattgg cgtcggggcg
     3601 gcggtggtag ccctcgaagg cttcgtcgcc caacaggtcg gccacgatct ggccttcttc
     3661 accggccgcg gcgaattggc cggggtcggt gctgtccagc ttcaccacgc gcagccgcgt
     3721 gttcagcccg gcgcggccgg cgctgccggg cttggccacg gcgcgctggt tgatgctgaa
     3781 ggtgtagacc tcgctgctgc cgtagtggtt gacgaagag
//
LOCUS       DAOORV010000268         3783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_28877_length_3783_cov_12.235783, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000268 DAOORV010000000
VERSION     DAOORV010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28877_length_3783_cov_12.235783"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKV56_18845"
     CDS             <1..465
                     /locus_tag="PKV56_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOX69906.1"
                     /translation="AEERGQSEAIGRNIFEMAQLEVPIVCTIIGEGGSGGALAISVGD
                     QTLMLQYSIYSVISPEGCASILWKTSERAADAAEALGVTAHRLKALGVIDKIVTEPVG
                     GAHRDHKQMASFLKRALSDALRQIGDLKPKELLQRRYERLQAYGRFTDSKER"
     gene            486..1787
                     /gene="tilS"
                     /locus_tag="PKV56_18850"
     CDS             486..1787
                     /gene="tilS"
                     /locus_tag="PKV56_18850"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOX69907.1"
                     /translation="MEDGRIAVAWSGGRDSTALLHATLVAAVPLGVEVLALHVHHGLS
                     PNADAWVAHCEQQTRRWARRGLPLRLCVTRLEAKPPRGESVEAWARRERYRALRQMAT
                     EQGAGIVLLAHHRLDQAETVLIQALRGGGVAGLSGMPREAERDGVRWLRPWLARAPEE
                     IAGYVRRHRLHHIEDESNADPRFARNRLRLEVWPSIVQAFPAAQTTLADTAAWAQEAT
                     ACLDELAALDLSAAASAKGLSVPAWLVLSVARRSNVLRQWLRGQFRQPAPASLVSRLM
                     EELGGARQGRWEHERGRLRLHHGILSWTPAADEPLRRGDREASLGLRRAGRHALPGWG
                     GHLVVSRVGEGGVPLAWLGHVELRARSGGEQFQAGIGRPPRSLKKQYQAQGVPAWERD
                     GPLLYSGGQLVFVPGLGLDARVIGLPGQPLVSLRWEPSAAG"
     gene            1850..3118
                     /locus_tag="PKV56_18855"
     CDS             1850..3118
                     /locus_tag="PKV56_18855"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOX69908.1"
                     /translation="MALIVHKYGGTSMGSTERIRNVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLALAKDLSPASITPEYQRELDMIASTGEQVSVGLLAIALQAEGMPAVSYAGW
                     QVPIATDSSYTKARIQSIDDQRVRADLAAGKVVVITGFQGVDGDNNITTLGRGGSDTS
                     AVAVAAALKADECLIYTDVDGVYTTDPRIVPEARRLHSISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPMRVLSSFTPWDIAIEEEARSGTLITFEEDAKMEQAVVSGIAFNRDEA
                     KISLLGVPDKPGIAYQILGPVADANIDIDVIIQNVSHDGKTDFSFTVHRNDYARTMEL
                     LKSTVVPRTGAAEVSGDTKICKVSIVGIGMRSHVGVASRMFKALSDEGINIQMITTSE
                     IKTSVVIDEKYMELAVRALHKAFDLEQAPA"
     gene            3156..3248
                     /locus_tag="PKV56_18860"
     tRNA            3156..3248
                     /locus_tag="PKV56_18860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3190..3192,aa:Ser,seq:gct)
     gene            complement(3304..>3783)
                     /locus_tag="PKV56_18865"
     CDS             complement(3304..>3783)
                     /locus_tag="PKV56_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69909.1"
                     /translation="LDYSRSFDRNGASYDTKLKFSTLGVYGDYFLGSSFRFTAGALIG
                     QRKASGNGVATNGTITINGTTYPAAGESVAAEARFPSFAPYLGIGFGHAQVSKGLSFY
                     FDAGVAIGKADAKITASPGLVAAAGQPNIDAEQRNLQDSVDALKFFPSVKFGLGYSF"
BASE COUNT          637 a         1233 c         1313 g          600 t
ORIGIN      
        1 gctgaagagc gaggccagtc cgaggcgatc ggccgcaaca tcttcgagat ggcgcagctc
       61 gaggtgccga tcgtgtgcac catcatcggc gagggtggct ccggcggcgc actggccatc
      121 agcgtgggtg accagacgct gatgctgcag tactccattt attccgtcat ctcgccggaa
      181 ggctgtgcgt cgatcctgtg gaagacttcc gagcgcgcgg ccgatgcggc cgaggcattg
      241 ggcgtcaccg cgcatcggct gaaagcgctc ggggtgatcg acaagatcgt caccgagccg
      301 gtcggtggcg cgcatcgcga ccacaagcag atggcctcgt tcctcaagcg cgcgctgagc
      361 gacgccctgc gccagatcgg cgacctcaag cccaaagagc tgctgcagcg acgctacgag
      421 cgcctgcagg cctacggccg cttcaccgac agcaaggagc gctgagcctg cgggccgcgg
      481 cggcggtgga agacggccgc atcgccgtcg cctggagcgg tggccgtgac tcgacggcgc
      541 tgctccatgc cacgctggtg gccgccgtgc cgctcggtgt cgaggtgctg gcgctgcatg
      601 tgcaccatgg cctgagcccg aacgccgatg cctgggtcgc gcactgcgag cagcaaaccc
      661 ggcgctgggc aaggcgaggc ttgccgcttc gtttgtgcgt gactcgtctc gaagcgaagc
      721 cgcctcgggg cgaaagtgtc gaagcctggg cgcgtcgcga gcgttaccgg gcgcttcgcc
      781 agatggcaac ggagcagggc gccggcatcg tcctgctggc gcatcaccgg ctggatcagg
      841 ccgagaccgt gctcatccag gccctgcgcg gcggcggcgt cgcgggcctg tccggcatgc
      901 cgcgcgaggc cgaacgcgac ggcgtgcgat ggctgcggcc ctggcttgca cgtgcgcccg
      961 aggagatcgc aggctacgtg cggcgccatc gcctgcacca catcgaagac gagagcaatg
     1021 cggacccgcg cttcgcacgc aaccgactgc gtctggaggt ctggccgtcg atcgtgcagg
     1081 cctttccggc agcgcaaacg acgctggccg acacggctgc ctgggcccag gaggccacgg
     1141 cctgcctcga cgaactggcg gccctggatc tgtcggcggc ggcgagcgca aagggcttgt
     1201 ctgtgccggc gtggctcgtg ctgagtgtcg cgcgccgatc caacgtgctg cggcagtggt
     1261 tgcgcggcca gttccgccag ccggcgccgg ccagcctggt cagtcgcctg atggaagaac
     1321 tgggcggcgc caggcaaggg cgatgggaac acgaacgagg gcggctgcgg ctccatcatg
     1381 gcatcctgag ctggactcct gccgctgacg agcccttacg gcgtggcgat cgcgaagcga
     1441 gcttgggcct gcgtcgcgcc ggccgccatg ccttgccggg ttggggtggt cacctcgtgg
     1501 tgtcccgggt gggcgagggc ggcgtgccac tggcctggct gggccacgtc gagttgcgcg
     1561 ctcgctcggg aggcgaacaa ttccaggccg gcatcggccg cccgccgcgc agcctgaaga
     1621 agcagtatca ggcgcaaggc gtgccggcct gggagcgcga tgggccgctg ctctacagcg
     1681 gcgggcagct cgtcttcgtg cccggcctgg gcctggacgc acgggtgatc ggcctgcctg
     1741 gtcagccgct ggtgagcctg cgctgggagc cttccgcggc cggctaaaat gcgcggttca
     1801 cgagcgcggc gtgaccccct tcgagcaatc accgcgagct tgcatcttca tggcactcat
     1861 cgttcacaag tacggcggca cgtcgatggg ctcgaccgag cgcatccgca acgtcgccaa
     1921 gcgcgtcgcc aaatgggcgc gggcagggca ccagatggtg gtcgtgccct cggccatgag
     1981 cggcgagacc aaccgcctgc tcgcgctggc caaggatctc tctcccgcct cgatcacccc
     2041 ggaatatcag cgcgagctcg acatgatcgc ctcgaccggc gagcaggttt cggtggggct
     2101 gctggccatc gcgctgcagg ccgaaggcat gccggcggtg agctacgcgg gctggcaggt
     2161 gccgatcgcc accgacagtt cgtacaccaa ggcgcgcatc cagagcatcg acgaccagcg
     2221 cgtgcgcgcc gacctggccg ccggcaaggt ggtcgtcatc accggcttcc agggcgtcga
     2281 cggcgacaac aacatcacca cgctgggccg cggcggatcc gacacctcgg cggtggccgt
     2341 ggccgcggcg ctgaaggccg atgagtgcct catctacacc gatgtcgacg gcgtctacac
     2401 caccgacccg cgcatcgttc cggaggcgcg ccgcctgcac agcatcagct tcgaggaaat
     2461 gctggagatg gccagtctcg gctccaaggt gctgcagatc cgctcggtgg agttcgccgg
     2521 caagtaccgc gtgccgatgc gcgtgctgtc gagcttcacg ccctgggaca tcgccatcga
     2581 ggaagaggcc cgctcgggca cgctgatcac tttcgaggaa gacgccaaaa tggaacaagc
     2641 cgtcgtgtcg ggaatcgcat tcaaccgcga cgaagccaag atcagcctgc tcggggtgcc
     2701 cgacaagccg ggcatcgcgt accagatcct cggcccggtg gccgatgcca acatcgacat
     2761 cgacgtgatc atccagaacg tctcgcacga cggcaagacg gacttctcgt tcaccgtgca
     2821 ccgcaacgac tacgcgcgca cgatggagtt gcttaagtcg acggtcgtac ccagaaccgg
     2881 tgccgccgaa gtgtcgggcg acacgaagat ctgcaaggtc agcatcgtcg gcatcggcat
     2941 gcgttcgcac gtgggcgtgg ccagccgcat gttcaaggcg ctgagcgacg agggcatcaa
     3001 catccagatg atcaccacca gcgaaatcaa gaccagcgtg gtgatcgacg agaagtacat
     3061 ggaactcgcc gtgcgcgcgt tgcacaaggc cttcgatctc gagcaggcgc ctgcctgaca
     3121 gcgccgcgat cgcggctaga atgcgagcct tcgccggagt cgtgaccgag tggccgaagg
     3181 tgctcccctg ctaagggagt atgtgggcaa aacctgcatc gagggttcga atccctccga
     3241 ctccgccaga ccgcaagtga aacgggcccc tcggggcccg ttttcattgg cgtgagcgac
     3301 ggctcagaag ctgtagccga gaccgaactt cacgctcgga aagaacttca gcgcgtcgac
     3361 gctgtcctgc aggttgcgct gctcggcgtc gatattgggc tggcccgccg cagccaccag
     3421 gcctggcgag gcggtgatct tcgcgtcggc cttgccgatc gcgacacctg catcgaaata
     3481 gaaactcagg cccttgctga cttgtgcgtg gccgaagccg atgccgagat acggggcgaa
     3541 tgacgggaac ctggcttccg cggctacgct ttcgccggcg gccgggtagg tggttccatt
     3601 gatggtgatc gtgccgttcg tcgcgacgcc gttgcccgac gccttgcgtt gcccgatcag
     3661 tgcgcccgca gtgaaccgga agctgctgcc gaggaagtag tcgccgtaga caccgagggt
     3721 ggagaacttc agcttggtgt cgtagctggc gccgttgcga tcgaagctgc ggctgtagtc
     3781 gag
//
LOCUS       DAOORV010000269         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_28927_length_3779_cov_9.413802, whole genome shotgun sequence.
ACCESSION   DAOORV010000269 DAOORV010000000
VERSION     DAOORV010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28927_length_3779_cov_9.413802"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PKV56_18870"
     CDS             complement(<1..1770)
                     /locus_tag="PKV56_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69910.1"
                     /translation="MPALQDIVTDRPAVPAGERAGDRGWFLLDSRWEDDVWILAPGNA
                     LEERQPVRLRWDFDLRDGRRFTDERYATLRETSRQLVALIRSRSLSTGLPLRPSTVAQ
                     YFHTLRCLLQWMDGEGFSRFGALDPPALLEFQQWLRTRPLTGHPSQRAPGTVQRHLYL
                     FAYFHRFRGELDDSVCFDPFAGHDQRQAAGYHEGLRRPWPYTPDTVAVALVQAAIDIL
                     TRDAPIVLRAWPTYRQAATGGRGAGHAHTGRATRALRSASAGIPDGDSPVRSVRELVL
                     RTDLLYAACFVVISYLVGPRVSEILHLKAGCVQHRCADGASEPVTVIVGAIFKRQSGY
                     HGRPHEWVAPPAAVQAVAVLEALSAGHRAVSGRDELWLRRWHGNGATEWHEVLPELLE
                     IPSIARINIQLQRFGAWLGLTHQDRPWRLSTHQGRKTFARFAALRDRTCLFALAQQLG
                     HRERAETDHGYAGSDYRLAQEIDAEILQQSVNAWEHMLATPGLGGRAGEEIMANRPRF
                     RGKRVKEDLASYARLLVDAGLVLGICDWGFCVYREEHSACLGNVAGPNPARREPSTCA
                     RCKNFVVSQRHRPYWVEQVHRCER"
     gene            complement(1781..3523)
                     /locus_tag="PKV56_18875"
     CDS             complement(1781..3523)
                     /locus_tag="PKV56_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69911.1"
                     /translation="MKSPRQSIRRVLSHEAVAAPAHDAQPARFDAPRVVRKTVVDFSS
                     LCLPEDVRLALAQAFWSHLGARSPRSIKSCWTSVLTFNRFAAESGAVASLADLDRRML
                     VRYVEWLNAQRRSDGRPWAISTRAGVYSALRRLLRWLERCRPDLVGHIDHSFTPFPGR
                     GRDDPSRATLPARELRAILRACEADIAAMREARASAAAQRAADGDKVGSLGWLLAEVD
                     RRFGGIVPSGLELQRRGNHPLQLAVRRWGGAKQIEPCLYPRAVSLLPYYLAIMIHTAG
                     NPEPIAELARDCLQEVPLLENRQALVWFKARAGRLQRRTFERDAPFEPPALIRDILAW
                     NEQLRPLAPPEQRNRLFIFKGLRTVNAMSTVTVHHLLAQFCERHGLAAFSLVSIRPSV
                     LCNFYRASGDLLRTRALANHANIATTVRYVEVPVVRRHNHVRIAALQEAFIEHLTAST
                     KSSPPVAAASPPSARLPSGELVSMFGFDCKDPLAGTAPGTRQGELCTNFMGCFTCPNA
                     VIAPEPLTVARLLQARDHLRAAAATLHPARWQAFYAPQLRILEEDILPRFAERELAAA
                     ESLLSQLPPLLDLR"
     gene            complement(3520..>3779)
                     /locus_tag="PKV56_18880"
     CDS             complement(3520..>3779)
                     /locus_tag="PKV56_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX69912.1"
                     /translation="VSQAGLACGFKATTHLLRATFACMLLARLEHLADRGAKINPLIV
                     VKVLLGHEHLDTTDRYLRAVAVDPCVLKDVLDTLLSETGAP"
BASE COUNT          627 a         1222 c         1369 g          561 t
ORIGIN      
        1 ccgctcgcag cggtggactt gctcgaccca atagggtcga tgccgctgcg acaccacgaa
       61 attcttgcag cgcgcgcagg tcgacggctc gcggcgagcg gggttcgggc cggccacgtt
      121 gccgaggcag gcgctgtgct cttcgcgata gacgcagaag ccccagtcgc agatgccgag
      181 cacaagcccc gcgtccacca gcaggcgcgc gtagcttgcg aggtcttcct tcacgcgctt
      241 gccccgaaag cgcgggcgat tggccatgat ctcctcgccg gcgcggccgc ccaggccggg
      301 tgtcgccagc atgtgttccc acgcgttgac cgactgctgc aggatctcgg cgtcgatctc
      361 ctgggcgagc cggtagtcgg acccggcgta gccatggtcg gtctcggcgc gctcgcgatg
      421 accgagttgc tgggccagcg cgaatagaca cgtgcgatca cgcaacgccg cgaacctggc
      481 gaaggtcttg cggccctggt gcgtggagag ccgccacggc cggtcctggt gcgtaaggcc
      541 cagccaggcg ccaaagcgct gcagctggat gttgatccgt gcgatcgacg gaatctcgag
      601 cagttcgggc aggacctcgt gccactccgt cgcgccgttg ccgtgccagc ggcgcaacca
      661 caactcgtcg cgtccggaga cggcgcggtg cccggcggac aaggcttcga gcaccgccac
      721 ggcctgcacc gcggcaggag gtgcaaccca ttcgtgcggc cgcccgtggt agcccgattg
      781 gcgcttgaag atcgccccga cgatcacggt gaccggctcg ctggcgccgt cggcgcaacg
      841 gtgctgcacg cagccggcct tcaagtgcag gatctcgctg acgcgcgggc cgaccaagta
      901 ggagatgacg acgaagcagg cggcatacag caagtcggtg cgcaggacga gttcgcggac
      961 cgatcgcacc ggggaatcac catcggggat gccggcgctg gcgcttcgaa gtgcccgtgt
     1021 cgctcttccg gtgtgggcat ggcccgcacc acgaccgccg gtcgccgcct gccgataagt
     1081 cggccaggcg cgcagaacga tgggagcgtc gcgggtgagg atgtcgatgg ccgcctgcac
     1141 caatgccacg gctacggtgt ccggcgtgta aggccagggt cgacgcagcc cctcgtgata
     1201 gccggcggcc tggcgctgat cgtgtcccgc aaaggggtcg aagcacacgc tgtcgtcgag
     1261 ttcgccgcga aagcgatgga agtacgcgaa caggtacagg tggcgctgca cggtccccgg
     1321 cgctcgctgc gaggggtggc cagtcagggg gcgcgtgcgc agccactgct ggaattccag
     1381 cagcgccggc gggtcgagcg ctccaaagcg gctgaagccc tcaccgtcca tccattgaag
     1441 caggcagcgc agggtgtgaa agtactgcgc caccgtgctc ggacgcagag gcaggccggt
     1501 ggacagcgag cgcgatcgga tgagggcgac gagctgcctg ctggtctccc gcagcgtggc
     1561 gtagcgctcg tcggtgaatc gtcggccgtc tcgcagatcg aagtcccagc gcaggcgcac
     1621 aggctgccgt tcttccagcg cgttgcctgg cgcaagaatc cagacatcgt cttcccacct
     1681 cgagtcgagc aggaaccatc cacgatcgcc ggcgcgttcg cccgcgggga cggcgggacg
     1741 gtcggtgacg atgtcctgca gggctggcat ggcagcggcc tcagcgcagg tccagcagcg
     1801 gcggaagttg cgacaacagc gactcggccg ccgcgagctc tcgttcggcg aagcgcggca
     1861 agatgtcctc ctcgaggatg cgtagctggg gcgcgtagaa ggcctgccag cgcgcgggat
     1921 gcagcgtcgc tgccgcggcg cgaaggtggt cacgcgcctg gagcagacga gccaccgtca
     1981 gcggctccgg cgcgatcacg gcgttggggc aggtgaagca gcccatgaag ttggtgcaga
     2041 gctcgccctg acgtgtaccc ggcgcggtgc cggccagcgg atccttgcag tcgaagccga
     2101 acatcgaaac cagctcgccc gatgggagcc gcgcgctcgg cggtgacgcc gcggccaccg
     2161 gcggagacga cttggtcgac gctgtgaggt gctcgatgaa ggcctcttgc aatgcggcga
     2221 tgcgcacgtg gttgtgcctg cggacgaccg gcacctcgac atagcggacg gtcgtcgcga
     2281 tgttggcgtg gttggccaaa gcccgcgttc gaagcagatc gccgctggcg cggtagaagt
     2341 tgcacaggac gctgggccga atgctcacca acgaaaacgc agcaaggcca tggcgttcgc
     2401 agaattgcgc gagcaggtgg tggaccgtga cggtggacat cgcgttcacg gtgcgcagtc
     2461 ccttgaagat gaagaggcgg ttgcgctgtt ccggtggtgc gagcggccgc aactgctcgt
     2521 tccaggcgag gatgtccctg atcagcgccg gcggctcgaa cggggcgtca cgttcgaacg
     2581 tgcgtcgctg caggcgtccg gcgcgggcct tgaaccacac cagcgcttgg cggttctcca
     2641 gcagggggac ctcctgcagg cagtcgcgcg cgagttcggc gatgggctcg gggttgccgg
     2701 ccgtgtggat catgatggcc aggtagtacg gcagcagcga gacggcacgc gggtacaggc
     2761 agggctcgat ctgcttggcg ccgccccacc gtcgcaccgc cagctgcaac ggatggttgc
     2821 cgcgccgttg cagctcgagg ccgctgggca cgatgccgcc gaagcgtcga tcgacctcgg
     2881 caagaagcca acccaaagag ccgaccttgt cgccatcggc agcgcgctgc gcggcggccg
     2941 atgcgcgcgc ttccctcatc gcagcgatgt ctgcttcgca agcacgcagg atggcgcgga
     3001 gctcgcgtgc cggcagcgtc gcgcgtgagg ggtcgtcgcg gccgcgccct ggaaagggcg
     3061 tgaaagagtg gtcgatgtgg ccgacgagat cggggcgaca gcgttcgagc cagcgcagca
     3121 gccggcgcaa cgcactgtag accccagctc gcgtggagat tgcccatggc ctgccgtccg
     3181 agcgccgttg ggcgttgagc cactcgacat agcgcaccag catccgtcgg tccaggtccg
     3241 cgagcgacgc gacggctccc gattcggccg cgaagcggtt gaaagtcagc accgaggtcc
     3301 agcatgactt gatgctgcgg ggcgaccggg ctccgaggtg gctccagaac gcctgtgcca
     3361 gcgcgagccg cacgtcctct ggcaagcaca gtgacgaaaa gtcgacgacc gtcttgcgaa
     3421 ccacacgagg ggcatcgaag cgagcgggct gcgcgtcgtg cgctggcgcc gccaccgctt
     3481 cgtgcgacag gacccgacgt atgctctggc gcggcgactt catggcgccc ccgtctccga
     3541 cagcagcgtg tcgagcacgt ccttgaggac gcatgggtcg acggcaacgg cgcgaaggta
     3601 gcggtcggtg gtgtcgaggt gctcgtggcc aagcagcacc ttcacgacga tcagagggtt
     3661 gatcttggcg cctcggtcgg ccaggtgctc gagccgggcg agcagcatgc acgcgaaggt
     3721 ggcccgcagc aggtgcgtgg tggccttgaa cccgcaggcc agacccgcct ggctcacgg
//
LOCUS       DAOORV010000270         3778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_28936_length_3778_cov_5.520817, whole genome shotgun sequence.
ACCESSION   DAOORV010000270 DAOORV010000000
VERSION     DAOORV010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28936_length_3778_cov_5.520817"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1643)
                     /locus_tag="PKV56_18885"
                     /pseudo
     CDS             complement(270..1643)
                     /locus_tag="PKV56_18885"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            2174..3022
                     /locus_tag="PKV56_18890"
     CDS             2174..3022
                     /locus_tag="PKV56_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOX69913.1"
                     /translation="MNKTASKISLPTRTISLGQPLAWLSRGYQDLARIGWLSLAHGLA
                     LAAFGAVILAVGHDRFWLLAGAISGFLVVAPVLATSLYALSRALERGEPPSVGVVTRT
                     WLNWQANHFGKWDRNYWRMVQFGCMLGIAATGWVLTSAALITLLAPAPIQTPMDFLHH
                     VVLAREGWLFELWLALGGVLAAPIFASSVVTMPLLLDRRITLRQAVLTSWQTVLANPI
                     AMGVWATVIAGLTVLGLCTFLLGLIPLIPLLGHASWHAYRDLVDTTDAPSQEPANGPL
                     TGEATR"
     gene            3022..3231
                     /locus_tag="PKV56_18895"
     CDS             3022..3231
                     /locus_tag="PKV56_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOX69914.1"
                     /translation="MFGYTEEQISSFGLTFGVGAFMLYMLFIIGQLAWESKAGKMGTF
                     VLFLGLGFGMVGFAAKFVIQWLITK"
     gene            3292..>3778
                     /locus_tag="PKV56_18900"
     CDS             3292..>3778
                     /locus_tag="PKV56_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX69915.1"
                     /translation="MNRTPADHRIPTGVWVLGFVSMLMDISSEMVHSLLPLFLVGTLG
                     VSVAAVGLIEGLAESTALVSKVFSGALSDYLGRRKGLAVLGYAMGALTKPVFAMANGV
                     GVVVLARFIDRVGKGIRGAPRDALLADIMPPGIRGAAFGLRQSLDTVGAFTGPLLATG
                     LM"
BASE COUNT          605 a         1242 c         1268 g          663 t
ORIGIN      
        1 tcgaccaagg cgaagctgtg cagaccacgc cactgatcgc ccttgtaaag cctgtcgtcc
       61 gcgctgaaag cccagcagcg gcgccattct tcccggccgt gtcccgcgtt gagcaccttg
      121 ctgcaggtgc tgccgcagtc gaggccggca gccaccgaac tgactgcgcc gctgcctgtg
      181 ccggtcttga ccaccgacag cgtgtagggg atcgccgtga aggtggccgt gacgctgcgc
      241 gcctgggtca tcgtcagcgt gcacggaccg gtgccggtgc aggcgccgcc ccagccggca
      301 aacgtcgacc cggcggccgt cgtcggggtc aatgtcaccg tcgtgccatc ggcatacggc
      361 tgactgcagt cgctgccgca aaggatgccg ctcggtgctg acgaaacgct gccgatgccg
      421 gtgccgctgc gcgtcacgct gagcgaatgg gcggtgccgg caagcaccgc gaggttgatg
      481 cgcgacatgc cggataccag cccgcccagg cgctggtcat tgaccgcgac ggcctgtcct
      541 cgaagcagcg ccagcgcgtc gtcaaccgac atggccggat tcgcctgctt cagcaacgcc
      601 caggcacccg cgacatgggg agccgccatt gaagtgccgt tctgcgccac gtagccgccg
      661 ccgggcaccg acgaggtgat gtagctgccc ggagccagca gcgaaacaaa ggatgcgatg
      721 ttggagtagg acgccacgcc gtcgacgccg gtggcgcacg cgctgccgtc gggcccggca
      781 tcgcacgtcg cgcccacgct cactgcgctg gagatgcacg ccggcgctgc gatcgcattc
      841 ttgtacccgc tgttgccgct cgcgatcacg gtcgcgatac cgaccgagcg caggttgtcg
      901 atgctggcct tcagcgccgt gttggtggcg tcgcaggtgg cggtgaactg tccggcgccc
      961 aggctcatgt tgaccgaggc gatctgatag gtacccctca gcgcatagac acgctcgagc
     1021 gccttgatct ggtcgctggt gtacgacatg acagcgttgc tcgccgggaa gcggctgaac
     1081 acctgcatcg cgatgatgtc ggcccctggc gcgacgccgc tcgacccgtc gggcgcaccg
     1141 ctgcccgcga cgatgccggc cacatgggtg ccgtgcgtgc aaccgctgac cgaggtgttg
     1201 cagttgaccc ccgaacccgc cgccgtgctg gcggtaacgc cacccgggca gaccgaactc
     1261 gattcgacgg tggtgctcga gtagcaggcc tccgatacca ccttgccagc caggaacgga
     1321 tgggtcttgt cgacgccggt atcgagcacc gcgaccgccc agccgctgcc ggtgcgcccg
     1381 gaattccagg ccaccggcga attgatcacg gtgttgcttt gcagcagcgt cggcggagac
     1441 gccacgtcct cctcgatgtt gcgcacgcgg cccgatgcgc gcagcctggc aaacgtgttc
     1501 gcgtccacct cggcggcgat gtgcggcacg ttgtcgaaga tggcgtggac cttggcaccg
     1561 gtccctccga gcaggtcggt gacggctgcg ttctgcgccg agcggatggc agcgcgctga
     1621 cggttcgcgt cgagtcgggt catccggcct tctggcacgg cgggcacatc gacctgcacg
     1681 atgacgcgca ggctgccccg ggcggcgacc ttctcggcca ctcgatcgag cgccgcggtc
     1741 ggcacgggct gcgcctgcac atcaggaagg cccagcaaga gcgatcccag catgaaggcc
     1801 gataccagga cccgtcgccg agcagagaaa aacgagcgcg agcgactcac catggcagga
     1861 ctccagtcca tggcgcagtg atgccgggcg gcatcggtca ccatggagac gacatttcaa
     1921 tcggtcgaaa agaggcataa cttgaggcca tttcggggac ttcgatcacg tcgacgggcg
     1981 ttgcgccatg attcggcgag agaaaagcgc cctcgccgcc cagaagccgg ggcaagtacg
     2041 agccggtaaa ctgaatgcgt cacttcctga tcgaaggtga atgccttgtt gtccggcttg
     2101 ctgcccgcat ccggcctcgg gccagcaacg gcatgccgtt cggcaagtcg gtcccgacac
     2161 tgaatccccc accttgaaca agaccgcctc gaaaatctcg ctgccgacac gcacgatctc
     2221 cctcgggcag ccattggcct ggctgtcccg cggctaccag gaccttgcac gtatcggctg
     2281 gttgagcctg gcgcacggcc tggcgctcgc cgctttcggt gccgtgatcc tggccgtggg
     2341 gcacgaccgg ttctggctgc tcgcaggggc catttcaggc tttctggtgg tggcgccggt
     2401 gctggcgacc agcctctatg cgctgagccg ggccctggaa cggggcgagc ccccgtcggt
     2461 cggcgtcgtg acccggacct ggctgaactg gcaggccaat cacttcggca aatgggacag
     2521 aaactattgg cgcatggtgc agttcggctg catgctgggc attgcggcca ccggctgggt
     2581 attgacctcg gccgcgctga tcaccttgct ggccccggca cccatccaga ccccgatgga
     2641 cttcctgcac catgtggtgc tggctcgcga gggatggctt ttcgagctgt ggctggcgct
     2701 gggtggcgtg ctggcagcac ccatcttcgc gtccagcgtc gtgaccatgc ccctgctgct
     2761 cgatcgccgg atcaccctga ggcaggcggt cctcacgagc tggcagaccg tgcttgccaa
     2821 cccgattgcg atgggggtgt gggcgacggt catcgcaggg ctcacggtgc tcggtctgtg
     2881 cacgttcctg ctcggcttga tcccgctgat tccgctgctg gggcacgcga gctggcacgc
     2941 ctatcgtgat ctggtggaca ccacggatgc gccctcgcag gaacctgcga acgggccgtt
     3001 gactggcgag gcgacacgct gatgttcggc tacaccgaag agcagatctc cagcttcggc
     3061 ctcacctttg gtgtgggcgc attcatgctc tacatgctgt tcatcatcgg acagctggca
     3121 tgggaatcca aggcggggaa gatgggcacg ttcgtcctct tcctcggact cggattcggc
     3181 atggtcgggt tcgccgccaa gttcgtgatc cagtggctga tcaccaaata ggctgacgcg
     3241 ggttcccggt ccgagaccca aacggggaga acgtccggtc gacaatggac catgaacagg
     3301 acgcctgccg accatcgcat cccgacgggg gtctgggtac tgggtttcgt cagcatgctg
     3361 atggacatct cctccgaaat ggtccacagc ctgctgccgc tgttccttgt cggcacgctg
     3421 ggcgtcagtg tggccgcggt cggattgatc gaaggactgg ccgagtcgac ggcgctggtc
     3481 tcgaaggtgt tctccggcgc actgagcgac tacctgggcc gacgcaaggg gctggccgtg
     3541 ctgggctatg cgatgggcgc gctgaccaag cccgtgttcg cgatggccaa cggggtcggc
     3601 gtggtggtcc tcgcccggtt catcgatcgg gtcggcaagg gcatacgtgg ggcaccacgc
     3661 gatgcactgc tggccgacat catgcccccg ggcatacgcg gtgcagcatt cgggctgcgc
     3721 cagtctctcg acacggtggg agccttcacg ggcccgctgc tggccacggg cctgatgc
//
LOCUS       DAOORV010000271         3741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_29297_length_3741_cov_5.075421, whole genome shotgun sequence.
ACCESSION   DAOORV010000271 DAOORV010000000
VERSION     DAOORV010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29297_length_3741_cov_5.075421"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1468)
                     /locus_tag="PKV56_18905"
     CDS             complement(53..1468)
                     /locus_tag="PKV56_18905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX69916.1"
                     /translation="MKISFLGAAREVTGSCYLIETATARFLVDCGMFQGGREASARNA
                     KPFAFDPRTLDFVLLTHAHIDHSGLLPKLTREGYAGPIYATGATVDLLAVMLPDSAHI
                     QEMEAERAKHRHKPAAESPASPPAADGATLPAYTMQDAVSCLQQLQAVHYDEEVAPHA
                     DVRCRFRDAGHILGSAILEIWVNEGEKTTKLVMSGDLGQPGRPILRDPTVIEDADLLF
                     IESTYGDREHKDQAATMEELVQVLEHTLARGNVIVPAFAVGRTQEVLYHLHRLTHEGR
                     LHNLNIYVDSPMAAAATRITMQHLALFDEAGQRLAKWHAAGRNLPSMRFTASVEESMA
                     LNKIRAGAIVISASGMCNAGRIKHHLRHNLGRRECSVLITGFQAQGTLGRSLVDGAKH
                     VSIFGEEIAVRAQIHTLGGFSAHAGQQALLAWASGFKRAPRQTFVVHGEEATALAFAD
                     RLRDERGWSVTVPAPGQIIKL"
     gene            complement(1468..2127)
                     /locus_tag="PKV56_18910"
     CDS             complement(1468..2127)
                     /locus_tag="PKV56_18910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOX69917.1"
                     /translation="MLGATRLYRNDPLPAVEAVDLLVVMGGPMNIYDEAKNRWLAKEK
                     RYIERAVSAGKRVLGICLGAQLVADVLGAQVYANADKEIGWFPVELTEAASASDLFAA
                     FPAQFEAFHWHGDTFDIPAGALHVARSAGCAHQAFVYDERVVGLQFHLETTPASARQL
                     IAHGTDEIVEGRYIQMPLTMLANPQRFDSINLAMHGLLDRLAADRSVRTVAAVAAPRR
                     A"
     gene            complement(2274..2681)
                     /locus_tag="PKV56_18915"
     CDS             complement(2274..2681)
                     /locus_tag="PKV56_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69918.1"
                     /translation="MSGIKQVPSLWTRAIALVAIGFGLLTIKEGGMTLSGDAAAVAAA
                     GNYVPFVLWFNFLAGFAYVIAGIGLWLRRPWAAWLSVTIAAATALAFTAFGIHVVSGA
                     AFEQRTVIAMTLRTLVWTAIAVIAWSRLLRRKA"
     gene            complement(2684..3004)
                     /locus_tag="PKV56_18920"
     CDS             complement(2684..3004)
                     /locus_tag="PKV56_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69919.1"
                     /translation="MTLGDPRPFDPEHLKKWWEVTCKYLMADSMSEPSAADLLGATSH
                     RMIRGQGSQVDTRSRVRDTSSVDGARLHLSADGQIGRCTCNSPGRIGPPITANADRQV
                     LTRK"
     gene            complement(3039..3497)
                     /locus_tag="PKV56_18925"
     CDS             complement(3039..3497)
                     /locus_tag="PKV56_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOX69920.1"
                     /translation="MRTLLVAATILVAGFALAAANAPAPAETDARAPKPLALRSIMKE
                     LGRNMQVITDGISREDWALIEKTAPRVADHPQPPMSERLRILAFAGTDAGRFKAYDGE
                     TNMAAEALAKGARAKDGEVTILAFQKLQSSCFNCHREFRKPFVEHFYGAQ"
     gene            complement(3529..>3741)
                     /locus_tag="PKV56_18930"
     CDS             complement(3529..>3741)
                     /locus_tag="PKV56_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX69921.1"
                     /translation="SGFMFPVASMPRVFQWLTLANPLRHYLEIVRAVFLKGAGLEALW
                     PQFLYLLAMGIGLLWFASTRFRKTAS"
BASE COUNT          655 a         1170 c         1207 g          709 t
ORIGIN      
        1 tcggcaggga gttcattcgg ttctccttct gggttgaggt cgtggcaatc gcctagagtt
       61 tgatgatctg gccgggcgca ggcaccgtca cgctccagcc gcgttcgtct cgcaatcgat
      121 cggcaaacgc gagtgccgtc gcctcttcac cgtgcaccac gaaggtttgg cgcggagctc
      181 gcttgaatcc ggacgcccag gccagcagcg cctgctggcc ggcatgcgcc gagaagccgc
      241 cgagggtgtg aatctgtgcg cgcacggcga tctcctcgcc gaagatgctg acgtgcttgg
      301 caccgtccac caaactgcgg ccgagcgtgc cctgggcctg aaagccggtg atcagcacgc
      361 tgcattcgcg ccggcccagg ttgtggcgca ggtgatgctt gatgcgcccc gcgttgcaca
      421 tgccgctggc tgaaatgacg atcgcacccg cgcgaatctt gttcagcgcc atggattcct
      481 cgacgctggc ggtgaagcgc atcgagggca gattgcggcc ggctgcgtgc catttggcca
      541 ggcgctggcc ggcttcgtcg aacaaggcca gatgctgcat cgtgatgcgg gtcgcggctg
      601 ccgccatcgg cgaatcgaca tagatgttca ggttgtggag ccggccttcg tgggtcaggc
      661 ggtgcaggtg gtacagcact tcttgcgtgc gccccacggc aaacgccggc acgatcacat
      721 tgccgcgcgc caaggtgtgc tcgagcacct gcaccagttc ttccatcgtt gccgcctgat
      781 ccttgtgttc gcggtcgcca taggtcgatt cgatgaacag caggtcggcg tcttcgatca
      841 cagtggggtc gcgaaggatc ggccggcccg gctgacccag atcgccagac atcaccagtt
      901 tcgtggtctt ctcaccttcg ttgacccaga tttcgaggat cgccgagccc aggatgtggc
      961 cggcatcgcg aaagcggcag cgcacatcgg catgcggtgc gacctcttcg tcgtagtgca
     1021 ccgcctgcaa ctgctgcaga cagctcacgg cgtcctgcat ggtgtacgcc ggcagtgttg
     1081 cgccgtcggc ggccggtgga cttgcaggcg actcggcagc gggtttatgg cgatgttttg
     1141 cgcgttcggc ctccatctcc tggatatggg cgctgtcggg cagcattact gccagcaggt
     1201 cgaccgttgc gcccgtcgcg tagatcggcc cggcatagcc ttcgcgtgtg agcttgggga
     1261 gcagcccgct gtgatcgata tgggcgtgcg tgagcaacac gaaatcgagc gtgcgtggat
     1321 cgaacgcgaa tggttttgcg ttgcgcgccg acgcttcgcg cccgccctgg aacatgccgc
     1381 agtcgaccag gaagcgggcg gtcgcggttt cgatcaaata gcacgacccg gtcacttcgc
     1441 gggctgcgcc gaggaaggaa atcttcatca tgctctcctg ggtgcggcga cagccgcgac
     1501 ggttcgaact gagcgatctg ctgccaaccg atcgagcaac ccgtgcatgg cgagattgat
     1561 gctgtcaaac cgctgcgggt tcgcgagcat ggtcaggggc atctggatgt agcgcccttc
     1621 gacgatctcg tcggtgccgt gcgcgatgag ctgccgcgcg ctggccgggg tcgtctccag
     1681 gtgaaactgc aagccgacga cccgttcgtc gtacacgaag gcctggtgtg cacagccggc
     1741 gctgcgcgcg acgtgcaacg caccggccgg gatgtcgaaa gtgtccccgt gccagtggaa
     1801 tgcctcgaac tgcgcgggga acgcggcaaa caggtcggac gcggacgcgg cttccgtcaa
     1861 ttcgaccgga aaccaaccga tctccttgtc ggcgttggcg tacacctgcg cgccgagcac
     1921 atcggcgacg agttgagcac caagacagat gccaagcacg cgtttccccg cggagaccgc
     1981 tcgttcgata taccgcttct ccttggcaag ccatcgattc ttggcctcgt cgtagatgtt
     2041 catggggccg cccatcacca ccagcaggtc gactgcttcg accgcgggca gcgggtcatt
     2101 tcgatacagg cgcgtggcgc cgagcacatg aggtccacgt cgaatccagc tctcgatgct
     2161 gccgagcccc tcgaagggca catgctggaa gcaatggatg ttcatggcgt tcgcctttgt
     2221 tgggggtgcc ggcgtcggtg ctgcagcatg cggccgaggc gactcttgcg atctcacgcc
     2281 ttgcgccgca gcagccggga ccaagcgatg accgcaattg ccgtccagac aagcgtgcgc
     2341 agcgtcatcg cgattaccgt tcgctgctcg aacgcagcac ctgaaaccac gtgaattccg
     2401 aaggccgtga acgcaagtgc cgtagctgcc gcgatggtga cggacagcca ggcggcccaa
     2461 ggtcgccgta gccacagccc gatgccggcg atcacatagg cgaagccggc caagaagttg
     2521 aaccacagca cgaacggcac gtaattcccg gctgccgcaa cggcggccgc gtcgccgctc
     2581 aaggtcatgc caccttcctt gatggtcagc agaccgaacc cgatcgcgac cagggcaatc
     2641 gcccgcgtcc agagactggg tacctgtttg attccactca tgatcatttc cttgtcaaga
     2701 cttgcctgtc ggcgttggcc gtgatgggtg ggcctattcg ccccggtgaa ttgcaagtgc
     2761 aacggccgat ctgaccgtcc gcgctcagat gaagccgagc cccgtcgacg gaggaggtat
     2821 cgcgtacccg cgaacgggta tcgacctgac tgccctggcc gcggatcatc ctgtgcgatg
     2881 tggcgcccaa aagatcggcg gcgctcggct cgctcatcga atcagccatc aggtacttgc
     2941 atgtgacctc ccaccacttc ttcaggtgtt cgggatcgaa tggtctcgga tcgccaagcg
     3001 tcatgctgct tctcccgtgt cagtgggctc gcggcggttc attgcgcacc gtagaaatgt
     3061 tcgacgaacg gtttgcggaa ttcccggtga cagttgaagc aactcgactg gagcttctgg
     3121 aacgcaagaa tggtgacctc gccgtccttg gccctggcgc ccttcgcaag cgcttcggcg
     3181 gccatgttgg tttcgccgtc ataggcttta aaccgacccg cgtcggtgcc cgcgaaggcc
     3241 aagatgcgca gcctttcgga catgggcggt tggggatggt cggcgacccg gggcgcggtc
     3301 ttctcgatca gcgcccagtc ctcgcgcgag atgccgtcgg taatgacctg catgttcctg
     3361 cccagttctt tcatgatgct gcgcagcgca agcggcttgg gggcccgtgc atcggtttcg
     3421 gccggtgcgg gcgcgtttgc agcggccagg gcgaacccgg cgaccaaaat cgttgccgcg
     3481 acgaggagtg ttctcatgaa tctcgtccat tgcgagttga aggcatgatc aactggccgt
     3541 cttgcgaaac cgcgttgacg cgaaccacag cagtccgatt cccatcgcca gcagatacag
     3601 gaactgcggc cacagggcct cgagacccgc gcccttgaga aagacggcgc gcacgatctc
     3661 gaggtagtgg cgcagcgggt tggccagcgt cagccactgg aagacccgcg gcatgctggc
     3721 caccgggaac atgaagcccg a
//
LOCUS       DAOORV010000272         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_29684_length_3705_cov_9.227671, whole genome shotgun sequence.
ACCESSION   DAOORV010000272 DAOORV010000000
VERSION     DAOORV010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29684_length_3705_cov_9.227671"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKV56_18935"
     CDS             <1..214
                     /locus_tag="PKV56_18935"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOX69922.1"
                     /translation="RPIAQVAVDGLDVNAEQVKRGFAWVFRRYSNDAALIALEAEAKS
                     AGVGLWADPHPIAPWEWRASRREGTD"
     gene            complement(215..973)
                     /locus_tag="PKV56_18940"
     CDS             complement(215..973)
                     /locus_tag="PKV56_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOX69923.1"
                     /translation="MSTGAKVGATLLGASECIKAPIHTPVSTAPKLELSSGSLEALKW
                     LALLLMTLDHVNKHLLHASVPELFAVGRLALPLFGFVLGYNLARPGALASGSYSRTAR
                     RLAIFGTIATIPFIALGGLGWGWWPFNIMATLLVATLCAWLIEVGGPARLVAAAAVFI
                     VGGALVEFWWPGLAVCLMAWAYCRRPSWLRLALWIGALASLYVINRNLWALAALPLIF
                     AASQVKINVPRGRLGFYVYYPAHLAVLWTLARLL"
     assembly_gap    1167..1266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1267..1964)
                     /gene="stbB"
                     /locus_tag="PKV56_18945"
     CDS             complement(<1267..1964)
                     /gene="stbB"
                     /locus_tag="PKV56_18945"
                     /inference="COORDINATES: protein motif:HMM:NF041292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HOX69924.1"
                     /translation="MKVCVLNFSGNVGKSTIAAHLLQPRLNAQVFSVESLNVDASSDG
                     VEVARLRGKHYNDLLQRLMKLDDAIVDVGSSNVEDFLKMMQHYADSQEEFDLFVVPVV
                     KDGKQQADTVNTIRALRAIGVPAAKILVLINKVETDDDLRTDFAGVFGFCASGEATIP
                     EGGVIFANELFAMLKTQQLSIGALNDDATDYRQRLRAAANEDEQDAAIQMIAMKRLAK
                     TCNKNLDTAFAALFP"
     gene            complement(1961..2452)
                     /locus_tag="PKV56_18950"
     CDS             complement(1961..2452)
                     /locus_tag="PKV56_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69925.1"
                     /translation="MDGRPMTPDAFSASLERSAAKHPPSKARRIAALLPQIEAAMAQG
                     SPRAHIVTALLDVGIEVTPHQLSNVIARLRRQREDAPGLPEARPALGAGIPESGATVL
                     PVPRIASVPTLAPALSAAPAATPASKFGAHDPRLLDEVMRSTPDMKALAKLAPKHPNQ
                     GKS"
     gene            2763..3068
                     /locus_tag="PKV56_18955"
     CDS             2763..3068
                     /locus_tag="PKV56_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69926.1"
                     /translation="MNPTFERALWASTPALRCLAPLDAILAPALGLYWIKTLPASGGL
                     VGAVLGVFCLWIGARRAYRALFEFEAYRWMTLRLAKLAIATWVVMAMVKLVWFIQGS"
     gene            3160..>3705
                     /gene="mobC"
                     /locus_tag="PKV56_18960"
     CDS             3160..>3705
                     /gene="mobC"
                     /locus_tag="PKV56_18960"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HOX69927.1"
                     /translation="MNTGRQASQRATLHVDVGPELSARVRAAAAAGGRTPSEWTRELL
                     QRELAQEAASADTGHQQQSGPDASAAAPGRLVLDAELASLLQQVQVQGQFRTRPAALR
                     LVLRHFIGQGPTPAAESVDATPLKDGVAALMRSNHELLPIGKNINQIAKSLNAMQGGF
                     RNTDTKNLQVFAQAVREHVEQA"
BASE COUNT          626 a         1136 c         1227 g          616 t
ORIGIN      
        1 ccggcccatc gcgcaggttg ccgtcgatgg cctcgatgtg aacgccgaac aggtcaagcg
       61 aggtttcgcc tgggtgttcc ggcgctactc gaacgacgcc gccttgatag cgctggaggc
      121 ggaggcgaag tctgcgggcg ttgggttgtg ggccgatcct caccccatcg cgccgtggga
      181 gtggagagcc tcccggcgcg agggtacaga ctgactacag cagccgcgcc aacgtccaga
      241 gcactgccag gtgcgccggg tagtagacgt agaagccgag ccggccgcgt ggcacattga
      301 tcttgacctg gctggctgca aagatgagcg gcaacgcggc aagcgcccac agattgcgat
      361 tgatgacgta cagcgaggcg agggcgccga tccacagggc cagcctcaac cagctgggcc
      421 gccgacagta ggcccaggcc atcaggcaga ccgcgagccc tggccaccag aactcgacca
      481 gcgcgccgcc gacgatgaag accgcggcgg ccgccaccag ccgcgccggt ccgccgacct
      541 cgatcagcca ggcgcaaagt gtggcgacca gaagcgtcgc catgatgttg aagggccacc
      601 agccccaacc cagtccaccg agcgcgatga atggaatggt ggcaatggtc ccgaagatcg
      661 ccaaccggcg cgctgtgcgc gagtagctgc cgctggccag cgcaccaggc cgggccaggt
      721 tgtagcccaa tacgaagccg aacagcggca aagccagtcg ccccaccgcg aacaactcgg
      781 gcaccgatgc gtgcagcaga tgcttgttga cgtgatcgag cgtcatcagc agcaacgcga
      841 gccacttcag cgcctccagc gagccgctgc tcaactccag cttcggggcc gtgctcaccg
      901 gcgtatggat cggcgctttg atgcattcgc tcgcgcccaa caacgtcgct cctaccttcg
      961 cgccggtact catgccgaga tcctttcatg acaagcaaag agccgtcgaa cgccaccacc
     1021 ggttcccgat ggcatataga gcttgcgcgg cagtccgggg gactgccgcg tgccccggta
     1081 ggagccggcc cggtagggct gcaacccgcc caagcgggat gcactccgta gcccggggca
     1141 ctcatgccag agcgcgcggt cgtggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnggga agagcgcagc gaacgcggta tcgaggttct tgttgcaggt cttggccagg
     1321 cgcttcatgg cgatcatctg gatcgccgcg tcctgctcgt cctcgttcgc cgccgcgcgc
     1381 aaccgctgcc ggtagtcggt cgcgtcgtcg ttcagcgcgc cgatggacag ctgctgcgtc
     1441 ttgagcatgg cgaacagctc gttggcgaag atcacgccac cttcggggat cgtcgcctca
     1501 ccgctggcgc aaaagccgaa gacgccggcg aagtcggtgc gcaggtcgtc gtcggtctcg
     1561 accttgttga tgagcaccag gatcttcgcg gccggcacac cgatggcccg aagcgcccgg
     1621 atggtgttca ccgtgtcggc ctgctgcttg ccgtccttga ccaccggcac cacgaagagg
     1681 tcgaactctt cctggctgtc ggcgtagtgc tgcatcattt tgaggaagtc ttcgacgttg
     1741 gacgaaccca cgtccacgat cgcatcgtcc agcttcatca gccgctgaag cagatcgttg
     1801 tagtgcttgc ctcgcagcct ggcgacctcg acgccatcgc tggaggcatc gacgttcagg
     1861 ctctcgacgc tgaagacctg ggcgttcagg cgaggctgca acaggtgggc ggcgatcgtg
     1921 ctcttgccga cgttgccgct gaagttcagg acgcagactt tcatgacttt ccttggttgg
     1981 ggtgtttcgg cgccaacttg gcgagtgctt tcatgtcggg cgtgctgcgc atgacctcgt
     2041 ccagcaggcg agggtcgtgc gcaccgaatt tcgatgccgg agtggcggcc ggtgcagctg
     2101 aaagtgcagg cgcgagcgtg ggaacgctgg cgatcctggg caccggcaag acggttgccc
     2161 cgctttccgg gattcccgcg ccgagcgccg gccttgcctc tggcaagcct ggcgcgtctt
     2221 cccgctgcct gcgcagacgg gcgatgacgt tcgagagctg gtgcggcgtg acctcgatgc
     2281 ccacgtccag gagcgccgtg acgatgtggg cgcgcgggct cccctgagcc atggccgcct
     2341 cgatctgagg caacagcgcc gcgatgcggc gagccttcga aggcgggtgc ttggccgcgc
     2401 ttcgttcgag gctggccgag aaggcgtccg gagtcattgg acggccgtcc atgagggacg
     2461 gctgagggca gggtgcgggt gcttggggtg catgttgcgt accttctgcc gtttgaacat
     2521 acaaacggtg caagagaagt gcgtaccgag acactatagg tgattgcaat gcaagaacag
     2581 tgcatataaa gtaaatgcaa gccagtcgaa ccggccttct tgtgcaacag agcgtacgtt
     2641 tggtgctctg gaagcaagct tttggaggcg gttcgagtgc gtcagggctc aaagcacgta
     2701 tggcgtgcgt atgagctggc gatctggcgc tgtccttgtg gtgtctgagt gagtaccaac
     2761 caatgaaccc gactttcgag cgagcgctgt gggcgagcac cccagcgctg cggtgcctgg
     2821 caccgctgga cgccatcctg gcgccggccc ttgggctgta ctggatcaag accttgcccg
     2881 cctctggcgg gctggtaggg gccgtgctgg gggtgttctg cctctggatc ggggccaggc
     2941 gcgcctatcg cgcgctgttc gaattcgagg cctaccgctg gatgacgctt cgcctggcga
     3001 agctcgccat cgcgacctgg gtcgtgatgg cgatggtcaa gctcgtctgg ttcatccagg
     3061 ggtcgtgatg cacgagggga tgcgacacca aacacagatg cgaaagagtg gagagcagga
     3121 taggctgcgc cggcttgttt gtgtcacgag gcctgcccga tgaacacggg caggcaggcc
     3181 tcgcaacggg cgacgcttca cgtcgatgtc ggccccgagc tgtcggctcg tgtccgggcc
     3241 gcggcggcgg ctggtggtcg caccccctct gaatggactc gcgaactcct ccagcgggag
     3301 ctggcacagg aggcggcatc ggcagacacc gggcatcagc agcagtcggg gcctgacgca
     3361 tcggcggcgg cacctggccg cttggtgctc gacgccgagc tggccagcct gttgcagcag
     3421 gtccaggtgc aggggcagtt ccgcacgcgc cctgcggcgc tgcgcctggt gctgcgtcac
     3481 ttcatcggcc aggggccaac gccggcggcc gagtcggtcg acgcgacgcc gctcaaggac
     3541 ggcgtcgctg cgctcatgcg ctcgaaccac gaactgctgc cgatcggcaa gaacatcaac
     3601 cagatcgcca agtcgctgaa cgcgatgcag ggcgggttcc gcaacaccga caccaagaac
     3661 ctgcaggtct tcgcgcaggc ggtgcgcgag cacgtcgagc aggcc
//
LOCUS       DAOORV010000273         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_29861_length_3688_cov_8.109551, whole genome shotgun sequence.
ACCESSION   DAOORV010000273 DAOORV010000000
VERSION     DAOORV010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29861_length_3688_cov_8.109551"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKV56_18965"
     CDS             <1..270
                     /locus_tag="PKV56_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOX69928.1"
                     /translation="WINECLTRAIALYLSGNTGRRIGTLFSDEADGPLDPEHKRMFMA
                     MKREVLRLGGYACEFFVSQTPELTGMADRIIDLEGMATQVAEEVV"
     gene            267..548
                     /locus_tag="PKV56_18970"
     CDS             267..548
                     /locus_tag="PKV56_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69929.1"
                     /translation="MTDHLPERCFVVLPGVPTRQNVFVCVRGELGVQATRMDFGDYEA
                     ATRVVRELNDALGVGPMAVRAMLVGCLTGWNRVQREFGFECRPMGATVH"
     gene            639..1193
                     /locus_tag="PKV56_18975"
     CDS             639..1193
                     /locus_tag="PKV56_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69930.1"
                     /translation="MAESEGNDDEGSAALTCAGVYLLQAHGTCYSCKQSTPMFGVMGL
                     PPFALEGGEYPVDEDECMFREIVEMPAQLAEAIRASAGPCFRPDFSRTAGALYWMNHC
                     KHCDAKQGDFFVHGPDGPFWPYDEAQMDAIQATRLDGPFRFVDPSTAYSGAMVEWRDR
                     KHGVQRPPPKAAVPRKKRKPKAGA"
     gene            complement(1290..1823)
                     /locus_tag="PKV56_18980"
     CDS             complement(1290..1823)
                     /locus_tag="PKV56_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69931.1"
                     /translation="MSYRIEYQWASWKLSASPETGGVDRFVIAIEGGDNNLRDAVTGK
                     RVRSWDVCMLGSAVQVLRQAVYFAGACEGGSLKPGSRDCSPEAYIRRIRRLIEEGAPP
                     ARGNWYPSIRVAEAHPLSTYAKHSGLAIEREQRYGDWLTRVLLTEGQRNLVFDFADRF
                     TDLRGWQLAAVSGLPAS"
     gene            complement(1825..2442)
                     /locus_tag="PKV56_18985"
     CDS             complement(1825..2442)
                     /locus_tag="PKV56_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69932.1"
                     /translation="MVIHLPDDATFYGERSYLPIAVDLVDGSKTPSLLRADQHGVHYM
                     AQYEVFSALCSDRAQRTVDQTLLVGGTPTTPERYLRLWRDAITTSVSAETAATLHGIR
                     AVAVLQAPLASVTGAKSSWTTCPFGSFTSFRAKYGGQMSLASDGTFALELDLARPGSA
                     RDAYYGASMMSSVLRSEPTAWRASIELRVTAGHHEQVPLFAGAEL"
     gene            complement(2580..3035)
                     /locus_tag="PKV56_18990"
     CDS             complement(2580..3035)
                     /locus_tag="PKV56_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69933.1"
                     /translation="MQFIEIGPVPGEENCAQVGSPDYTEASLRECEVFRRMLYRLFPV
                     PEGLPVAYIGRTHPHDFGNYREVSIRYDDANAQAVDFAYEVESSAPAAWDTIARYELA
                     WYERKQAYDLAVREHRLRPEEVPAQFATATPPALAPNASFSELLASNPL"
     gene            complement(3115..3312)
                     /locus_tag="PKV56_18995"
     CDS             complement(3115..3312)
                     /locus_tag="PKV56_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69934.1"
                     /translation="MNELTDCVGDHWVPACGGTERPTKTRTGRTLLYMWNTTKGEHAY
                     YDCERDIFLTDDEARAALALD"
     gene            complement(3309..>3688)
                     /locus_tag="PKV56_19000"
     CDS             complement(3309..>3688)
                     /locus_tag="PKV56_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOX69935.1"
                     /translation="LIDSPTVMKDFLRVYFAGAERETFVVVFLDANHRVIDVEELFSG
                     TLTQTSVYPREVVKRALHFNAAAVCLSHVHPSSQTEPSRADEFLTSTLRQALSLIDVR
                     IIDHIIVAANGSSLTSMAERGLL"
BASE COUNT          640 a         1105 c         1291 g          652 t
ORIGIN      
        1 tggatcaacg agtgcctgac acgtgcaatc gcgctctact tgtccggcaa caccggccgc
       61 aggattggca cgctgttcag cgacgaggcg gacggtccct tggatccgga gcacaaacgc
      121 atgttcatgg ccatgaagcg cgaggtgctg cggctcggcg gatacgcctg cgagttcttc
      181 gtgagccaga ccccggagct caccggcatg gccgatcgca tcatcgactt ggaaggtatg
      241 gcaactcagg ttgccgagga ggttgtgtga ctgaccactt gcccgagcgc tgctttgtgg
      301 tgctcccggg agttccgacg cgccagaatg tcttcgtttg tgtccggggc gaactcggtg
      361 tgcaggcgac ccgcatggac ttcggcgact atgaagccgc gactcgcgtg gtccgcgagc
      421 ttaatgacgc gctgggcgtt gggccgatgg cggtgcgcgc gatgctggtc ggctgcctga
      481 cgggttggaa ccgcgtgcag cgagagttcg ggttcgaatg ccgccccatg ggtgccaccg
      541 tccattgacg gtcgaaggag tgaacgccga acggggccat cgcacggtga ggatcgttgc
      601 tcggcggtaa gcttgtgcga tgagggggtc ggcaatcgat ggctgagagc gaaggaaacg
      661 atgatgaggg gtccgctgcg ctgacttgcg ccggcgtgta cctgctgcag gcacacggga
      721 cgtgctactc gtgcaagcag tcgaccccaa tgttcggggt gatgggcctg ccgccgttcg
      781 cgctggaggg aggggagtac ccggtcgacg aagacgagtg catgtttcgg gagatcgtgg
      841 agatgcccgc gcagcttgcg gaggcgatca gggcaagcgc aggcccatgc ttccggcccg
      901 acttcagtcg tactgctggc gctttgtact ggatgaacca ctgcaagcat tgcgacgcca
      961 agcagggcga cttctttgtc catggccccg atggaccgtt ctggccctac gacgaagcgc
     1021 agatggacgc gatacaagca acccggctcg atggtccatt caggttcgtc gatccaagca
     1081 cggcgtactc gggggcgatg gtcgagtggc gagaccgcaa gcacggtgtc caacgtccac
     1141 caccgaaagc ggcggtgcca cgaaagaagc gcaagcccaa agcgggcgct tgaggccgag
     1201 ccgctgctga cggcctctct ggagcctccg cgtgacaaga ggttctagag aagcccctga
     1261 gctgtcgccc aggggaaagg atctcggatt cagctcgcag gcaagccgct cacggctgcc
     1321 agttgccagc cacgcaggtc tgtgaacctg tcggcgaagt cgaagacgag gttgcgttgc
     1381 ccttcggtga gcaagacccg cgtcaaccag tcgccgtagc gctgctcacg ctcgatggca
     1441 aggccggagt gcttggcata ggtcgacagc gggtgcgcct cggcaactcg aatggacggg
     1501 taccagttgc ctcgagctgg cggcgcgcct tcttcgatca ggcggcgaat gcggcggatg
     1561 taggcctccg gtgagcagtc ccgcgagcca ggcttcaggg agcccccctc gcacgctccg
     1621 gcgaagtaaa cggcctgccg gagaacctgc acggcgctgc cgagcatgca gacatcccag
     1681 ctgcgaacgc gcttgcccgt cacggcatct cggaggttgt tgtcgccgcc ctcgatcgcg
     1741 atgacgaacc ggtcgacacc acctgtctcg gggctcgctg acagcttcca gctggcccat
     1801 tggtactcga ttcggtacga catgtcagag ctccgcgccg gcgaacagcg gaacttgttc
     1861 gtggtggcca gcggtcacgc ggagctcgat ggatgcacgc catgcggttg gctcgctccg
     1921 caggacggag ctcatcatcg aggccccgta gtaggcgtcg cgtgcactgc caggcctggc
     1981 caggtcgagt tcgagcgcga aggttccgtc gctcgccaga ctcatctgtc cgccgtactt
     2041 ggctcggaag gaggtgaagg atccgaaggg gcaagttgtc caggaggact tcgcgccggt
     2101 cactgacgcg agcggtgcct ggaggacggc gacagcccgg atgccgtgca gagttgctgc
     2161 ggtctcggcg gagaccgaag ttgtgatcgc gtcccgccac agcctgaggt agcgctccgg
     2221 cgtcgtcggc gtgccgccaa ccagaagcgt ctgatcgacg gttcgttggg cgcggtcgct
     2281 gcacagggcc gagaagacct cgtactgggc catgtagtgg acgccgtgtt ggtcggcacg
     2341 caacaacgag ggtgtcttcg agccgtccac cagatcgacc gcaatcggca ggtaagacct
     2401 ttcaccgtag aaggtggcgt cgtcgggcag gtggatgacc aaccggccga cttcatcgag
     2461 cgttggggag tgaggactca tcaagttctc cttgaaggtg aaaagggcgc tgccgtgtgc
     2521 ggcgagcgcc ctttcaggga tggtgggaat gctgtctcag cggtcagcag gcggacgcct
     2581 cacagtgggt tgctcgccag cagctcggag aagctggcgt tcggcgccag ggccggcggc
     2641 gtcgcggtgg cgaactgggc cggaacctcc tcgggccgca ggcggtgctc tcgcaccgca
     2701 aggtcgtatg cctgcttgcg ctcgtaccag gccaactcgt accgagcgat cgtgtcccac
     2761 gcggccggcg ccgagctttc gacctcgtag gcgaagtcga cggcctgggc gttggcgtcg
     2821 tcgtaccgga tcgagacttc gcggtagttg ccgaagtcgt gtggatgggt gcgtccgatg
     2881 taggccaccg gcaacccttc gggcaccggg aacagccgat acagcatgcg gcggaagacc
     2941 tcgcactcgc ggagcgaggc ctcggtgtag tcgggactac ccacctgggc gcagttctcc
     3001 tcgcccggaa ccgggccgat ttcgatgaat tgcatgaaga tgctccaaga ggggtgcgcc
     3061 ttgttggcgc accgggtgga aggagtaggg cgtgcggcgg gaccggctgc acactcagtc
     3121 gagggcgagt gccgcgcggg cctcgtcatc ggttaggaag atgtcgcgct cacagtcgta
     3181 gtacgcgtgc tcgcccttgg tggtgttcca catgtagagc agcgttcggc ccgtgcgcgt
     3241 cttcgtcggc cgctcggtgc cgccgcacgc ggggacccag tgatcgccaa cgcagtcggt
     3301 cagctcgttc acagcagtcc ccgctcggcc atggaggtca acgagctgcc gttggctgcg
     3361 acgatgatgt ggtcgatgat cctcacgtcg atcaacgaca gcgcctggcg cagcgtgctg
     3421 gtgaggaact cgtcggcccg gctaggctcg gtctgcgatg acgggtgcac gtggctcagg
     3481 cacacggccg ccgcgttgaa gtgcagcgcc cgcttgacca cttcccgcgg atagaccgag
     3541 gtctgcgtca gcgtgccgga gaacaactcc tccacgtcga tgacgcgatg gtttgcgtcg
     3601 aggaagacca cgacgaaggt ctcgcgttcg gctccggcga agtacacccg caggaagtcc
     3661 ttcatgaccg tgggcgagtc gatcaggg
//
LOCUS       DAOORV010000274         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30068_length_3668_cov_10.442845, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000274 DAOORV010000000
VERSION     DAOORV010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30068_length_3668_cov_10.442845"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /gene="dcd"
                     /locus_tag="PKV56_19005"
     CDS             complement(<1..74)
                     /gene="dcd"
                     /locus_tag="PKV56_19005"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOX69936.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFEPG"
     gene            complement(158..1618)
                     /locus_tag="PKV56_19010"
     CDS             complement(158..1618)
                     /locus_tag="PKV56_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HOX69937.1"
                     /translation="MIDDIDASSETVGPAQSSAVTPLARWLREGARTAVFMRPRTEGL
                     QAGPSTLLMLLVLLVLCGIGLQRWALDEPARFDATAIASGWLATVLTLGTCWVVARSR
                     ISAAEQRVQAPATPTLFGLLLAQELALALPLGLAYLAALQWAPQPLPDEVDAWPEALW
                     MLSLLWIALAAAVVLWRQTSRPGLARLAIVLNATVTLMLPVAAPAPPYWVLVEPQRAP
                     LAAGEAPAFIPSQAILEAQVKALPAALDRLQPARPGLVDLYAITFGPFAEEDVFSREA
                     SLVSELMRTRFDARQRVVQLQNHSSTAGDVPWATPLNLQRSIERIAALMNLDEDILFI
                     HLTSHGARDGRLAASFEPLAIDEVTPQQLAGWLDAAGIRYRVISISACYSGSWIEPLA
                     GPGTLVMTAADALHTSYGCGRKSELTFFGRAMYDEQLRHTHSFEAAHAAVRPLIDQRE
                     REAGKTDGYSNPQISVGAAIRGPLLSLEQRLDAMSP"
     gene            complement(1626..2483)
                     /locus_tag="PKV56_19015"
     CDS             complement(1626..2483)
                     /locus_tag="PKV56_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOX69938.1"
                     /translation="MALPPRLTAARAALTHEIAALDEFGAMRRISIPAERPLTVFVDK
                     RELVTLMTLGAAPELLVLGYLRNQRLVDALDDIESVTVDWDVSAAAVRTHRGIERFDE
                     KTARRVVTTGCGQGTVFGNLMSELDSLQLPPASEPTARLGQDKLYGLLNTMRIQESTY
                     KSAGSVHGCALFRQDELLTFVEDVGRHNAIDTIAGWMWMNGVGGADKIFYTTGRLTSE
                     MVIKSAQMGVPIIVSRSGITQMGHAVAQQLGLCLFGRATNRHFICYSGFDRFDSRPEP
                     VAAGTGRAA"
     gene            complement(2506..3060)
                     /locus_tag="PKV56_19020"
     CDS             complement(2506..3060)
                     /locus_tag="PKV56_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HOX69939.1"
                     /translation="MKIRSFLSALGASAVLLSGCALLQPPAPVAPLKDGELAIPADYK
                     SWPKFLSEVQRPDAKQVRDIYMNPVATQGTQAGGFPNGSVFVMENYAAKVDADGKPLV
                     GADGKLVKGNLLRVFLMGKNEGWGQSAPEGLKNGDWIYAGYLGNGDKAPEPASACRTC
                     HLPLTQSKDFVYRYDEYFGKVASK"
     gene            complement(3166..>3668)
                     /locus_tag="PKV56_19025"
     CDS             complement(3166..>3668)
                     /locus_tag="PKV56_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOX69940.1"
                     /translation="LRQTNWGDLDYLIVDMPPGTGDIQLSLSQKVPLTGAVIVTTPQD
                     IALLDAKKGIKMFEKVGVPILGIVENMAVHVCEKCGHVEHIFGEDGGKHLAAEYKMDY
                     LGALPLNLSIRVQADNGRPTVVSDPDGEIAGLYKGVARQVAVKIAQRAKDFSSKFPSI
                     TISKDT"
BASE COUNT          624 a         1233 c         1219 g          592 t
ORIGIN      
        1 ccgggctcga acggctcgat catgccgtgc tgctcggcca tgcggcggat ccacttgtcg
       61 ctcttgatgc tcatgtgtca caccgtcgag gtgcctctgg gaaaacgggg gagaggtggc
      121 tgggaacgat tgtagggagc gggtcgtcgg cccgacgtca cggcgacatc gcgtcgagcc
      181 gctgctcgag gctgagcaac ggcccccgga tggcagcgcc gaccgatatc tgcgggttgg
      241 aatagccgtc tgtctttccg gcttcgcgct cgcgctgatc gatcagcgga cgcaccgcgg
      301 catgtgcggc ctcgaaggaa tgggtgtgcc gcagttgctc gtcgtacatc gcgcggccga
      361 agaaagtcag ctcggacttg cgcccgcacc cgtaggaggt gtgcagcgcg tcggccgccg
      421 tcatgacgag cgtgccgggc ccggccagcg gctcgatcca cgagcccgaa tagcacgccg
      481 agatcgagat cacccggtag cgaatcccgg ctgcgtcgag ccacccggca agctgctgcg
      541 gcgtgacctc atcgatggcc agcggctcga agctggcggc cagccggccg tcgcgcgcgc
      601 catgcgaagt caggtggatg aagaggatgt cctcatcgag gttcatcagc gcggcgatgc
      661 gctcgatgct gcgctggaga ttcagcggag tggcccaggg cacgtcgcct gcagtgctcg
      721 aatggttctg caactgcacg acccgctgcc gggcatcgaa ccgcgtgcgc atcagctcgg
      781 acaccagtga cgcttcgcgg ctgaagacgt cttcctcggc aaacggcccg aaggtgatcg
      841 cgtacaggtc gacgaggccg gggcgtgcgg gctgcagtcg gtccagcgcc gcgggcagcg
      901 ccttcacctg cgcctcgagg atcgcctggc tcggaatgaa ggcgggcgcc tccccggcag
      961 caagcggcgc gcgctgcggc tcgaccagga cccagtaggg cggcgccggc gcagcgaccg
     1021 gcagcatcaa ggtgaccgtg gcgttcagca cgatggccag gcgcgcgagc cctggtcgcg
     1081 aggtctgtcg ccacagcacc acggccgccg ccagcgcgat ccacagcagc gagagcatcc
     1141 acaaggcttc cggccaggca tccacctcgt cgggcagcgg ttgcggcgcc cactgcaaag
     1201 ccgccaggta ggccagaccc agcggcagcg cgagcgccag ttcctgggcc agcagcagcc
     1261 cgaacagcgt cggcgtggcg ggcgcctgca cacgctgttc ggctgcgctg atgcgactgc
     1321 gcgcaacaac ccagcaagtg cccaacgtca gcacggtggc cagccagccg ctcgcgatcg
     1381 cggtcgcgtc aaaccgggcg ggctcgtcga gcgcccagcg ctgcaggccg atgccgcaca
     1441 acaccagaag caccagcaac atcagcaacg tggatggtcc ggcctgcaac ccctcggtgc
     1501 gcggcctcat gaagaccgcc gtgcgcgcac cctcgcgcaa ccagcgagcg agcggcgtga
     1561 ctgcagaaga ctgcgcaggc cccaccgttt cggacgaagc gtcgatgtcg tcgatcatgc
     1621 gcggatcacg ctgcgcgccc ggtgcccgca gccactggct cgggccggga atcgaagcga
     1681 tcgaacccgc tgtagcagat gaaatgccgg ttcgtggcgc ggccgaaaag gcacagcccc
     1741 agctgctgtg caaccgcatg ccccatctgc gtgatgccgc tgcgcgacac gatgatgggc
     1801 acacccatct gcgcggactt gatcaccatc tcgctggtca ggcggcctgt cgtgtagaag
     1861 atcttgtcag caccgcccac gccgttcatc cacatccagc cggcgatggt gtcgatcgcg
     1921 ttgtgacggc cgacgtcctc gacgaaggtc agcagctcat cctgccggaa cagcgcgcag
     1981 ccatgcaccg agccggccga cttgtacgtg ctttcctgga tcctcatggt gttcagcagt
     2041 ccgtagagct tgtcctggcc cagccgcgcc gtgggctcgc tcgccggagg caactgcagc
     2101 gagtcgagtt cgctcatcag gttgccgaac acggtgccct gcccgcagcc ggtggtgacg
     2161 actcgcctgg cggtcttctc gtcgaagcgc tcgatgccgc gatgcgtgcg caccgcagca
     2221 gcgctgacgt cccagtcgac ggtgacggac tcgatgtcgt ccagcgcgtc gaccagccgc
     2281 tggttgcgca ggtagccgag caccagcaat tcgggcgcag ccccgagcgt catcagcgtg
     2341 acgagttcgc gcttgtcgac gaagaccgtc agcggccgct cggcggggat cgagatgcgg
     2401 cgcatcgcgc cgaactcatc cagcgcagcg atctcgtgcg tcagcgcagc gcgagccgcg
     2461 gtcaggcgag gcggcagcgc cattcagagc tgtcgcaacg ggcgcttact tggaagcgac
     2521 cttgccgaag tattcgtcat agcggtacac gaaatccttg ctctgcgtca gcggcaaatg
     2581 gcaggtgcgg caggccgagg cgggttccgg tgccttgtcg ccattgccca ggtagcccgc
     2641 atagatccag tctccgttct tcaggccctc gggggccgat tgaccccagc cctcgttctt
     2701 gcccatcagg aacacgcgca gcaggttgcc cttgaccagc ttgccgtcgg cgcccaccag
     2761 tggcttgccg tccgcatcga ccttggctgc gtagttctcc atcacgaaca ccgagccgtt
     2821 ggggaatcct ccggcctggg tgccctgcgt ggcgacgggg ttcatgtaga tgtcgcgcac
     2881 ctgcttggca tcggggcgtt gcacctccga caggaacttc ggccagctct tgtaatccgc
     2941 cgggatcgcc agttcgccgt ccttcagggg agcaacaggg gcgggcggct gcaacaacgc
     3001 gcagcccgac aacagcacgg cggaggcgcc cagcgccgag aggaatgagc gaatcttcat
     3061 gcaagtctcc ttggaagggt gggtgacaac gagagtggta gggcggtagc cgcagcgatt
     3121 gcaccgtcgc ggaggccctg gctgcagacg tggaccggaa cgagatcagg tgtccttgga
     3181 aatcgtgatc gacgggaact tggacgagaa gtccttggcc cgctgcgcga tcttcaccgc
     3241 aacctgacgc gccacgccct tgtacagacc ggcgatctcg ccatccggat cgctgaccac
     3301 cgtcggcctc ccgttgtccg cctgcacgcg gatgctcagg ttcaacggca gcgcgccgag
     3361 gtagtccatc ttgtactcgg ccgccaggtg cttgccgccg tcctcgccga agatgtgctc
     3421 cacgtggccg catttctcgc agacatgcac cgccatgttc tcgacgatgc cgaggatggg
     3481 cacgcccacc ttctcgaaca tcttgatgcc cttcttggcg tcgagcagcg cgatgtcctg
     3541 cggcgtggtc acgatcaccg cgccggtcag cggcaccttc tgcgaaagcg acaactggat
     3601 gtcgccggtg cccggcggca tgtcgacgat caggtagtcg aggtcacccc agttggtctg
     3661 gcgcagca
//
LOCUS       DAOORV010000275         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30328_length_3645_cov_5.539554, whole genome shotgun sequence.
ACCESSION   DAOORV010000275 DAOORV010000000
VERSION     DAOORV010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30328_length_3645_cov_5.539554"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="pnp"
                     /locus_tag="PKV56_19030"
     CDS             <1..1142
                     /gene="pnp"
                     /locus_tag="PKV56_19030"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOX69941.1"
                     /translation="IEIRTGVLPRTHGSALFTRGETQAVVVATLGTDRDAQRIDALAG
                     EFEERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIAVLPSKEDFPYTVRVV
                     SEVTESNGSSSMASVCGGCLSMLDAGVPLKAHVAGIAMGLIKEGNRFAVLTDILGDED
                     HLGDMDFKVAGTNGGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMVEAMGSA
                     NTEVSQFAPRLYTMKINPEKIRDVIGKGGATIRALTEETGTTIDIGEDGTITIASTDA
                     DKAAHAKKRIEEITAEVEIGKVYEGPIVKILDFGALVNLLPGKDGLLHISQIAHQRVE
                     KVTDFLKEGQIVKVKVLETDEKGRVKLSMKALIDRDQQPAAGSQE"
     gene            1224..2210
                     /locus_tag="PKV56_19035"
     CDS             1224..2210
                     /locus_tag="PKV56_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOX69942.1"
                     /translation="MKAIEISAFGAPEMLRLTERPAPIAGPGEVLIAVTASGINRPDV
                     LQRKGIYPPPVGASDLPGLEVAGSIVSGDERAMTEAGLRVGDRVCALVAGGGYAELCV
                     APVGQCLPVPAGLTDIEAASLPETCFTVWSNVFARAGLKAGETLLIQGGSSGIGVTAI
                     QFARAAGATVLVTVGSDEKAAACLNLGAHHAINYRTEDFVGAVKRLTAGRGVDVILDM
                     VAGDYVAREVSCLADDGRLVIIAVQGGSAAQFDASRLLMRRLTITGSTLRARSVEFKS
                     AIARELRSTAWRWIESRLVKAVVFRIFPAAQAAQAHALMESNQHIGKLVLSW"
     gene            2204..3013
                     /gene="tpiA"
                     /locus_tag="PKV56_19040"
     CDS             2204..3013
                     /gene="tpiA"
                     /locus_tag="PKV56_19040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOX69943.1"
                     /translation="MVNSQLSKPGAGRRPLVVGNWKMNGTRASSVQLLSALRSAEPFD
                     SDVSVCVPFPYLGEVALTLQGSRITWGAQDCSHLEPGAHTGDVAAVMLADFGCRHVIV
                     GHSERRVHHAESDQRVADKAKTALSHGLTPIVCVGETLEERQAMQTEAVVKRQLSAVI
                     HTLGHCVSQIVVAYEPIWAIGTGLTATPEQAQAVHAVLRAQLAAASTQAVDMRLLYGG
                     SVTAANASALFAQSDIDGGLVGGASLKANEFSAICRAASAAVHESLGKSKT"
     gene            3010..3450
                     /gene="secG"
                     /locus_tag="PKV56_19045"
     CDS             3010..3450
                     /gene="secG"
                     /locus_tag="PKV56_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOX69944.1"
                     /translation="MNLMLNLVVIVQILAALVMIGLVLVQHGKGADMGASFGSGASGS
                     LFGATGSANFLSRSTAVCAAVFFACTLLLAFLANDRSRAAPTGSVLDRPAISAPDAGA
                     SGATGATQIPGGAGIAPAPATVSASAAAATSDAPASGAAAIPSK"
BASE COUNT          628 a         1117 c         1229 g          671 t
ORIGIN      
        1 cgatcgagat ccgcaccggc gtgctgccgc gcacccacgg ctcggcgctg ttcacgcgcg
       61 gcgagacgca ggcggtggtg gtggccacgc tgggcaccga ccgcgacgcc cagcgcatcg
      121 acgccctggc cggcgagttc gaggagcgct tcatgctcca ctacaacatg ccccccttcg
      181 ccactggcga aacgggccgc gtgggcagcc ccaagcgtcg cgaaattggc cacggccgcc
      241 tggccaagcg tgcgctgatc gccgtgctgc cgagcaagga agacttcccc tacaccgtgc
      301 gtgtggtgtc cgaagtgacg gagtccaacg gctcgtcgtc catggcttcg gtctgcggcg
      361 gttgcctgtc catgctggac gcgggcgtgc cgctgaaggc gcacgtggct ggcatcgcca
      421 tgggcctgat caaggaaggc aaccgcttcg cggtgctgac cgacatcctg ggtgacgagg
      481 accacctcgg cgacatggac ttcaaggtgg ccggcaccaa cggtggcatc accgcgctgc
      541 agatggacat caagatccag ggcatcacca aggaaatcat gcaggtcgca ctggcgcagg
      601 ccaaggaagc gcgtctgcac atcctgggca agatggtcga ggccatgggt tcggccaaca
      661 ccgaggtgtc gcagttcgcc ccgcgcctgt acacgatgaa gatcaacccc gagaagatcc
      721 gtgacgtgat cggcaagggt ggcgccacca tccgcgccct gaccgaggag acgggcacga
      781 cgatcgacat cggggaagac ggcacgatca cgatcgcatc gaccgacgcc gacaaggccg
      841 cgcacgccaa gaagcgcatt gaggagatca ctgccgaggt cgagatcggc aaggtctacg
      901 aagggccgat cgtcaagatc ctggacttcg gggccctggt caatctgctt ccgggcaagg
      961 acgggctgct gcacatcagc cagatcgcac atcagcgcgt ggagaaggtc accgacttcc
     1021 tgaaggaagg ccagatcgtc aaggtcaagg tgctcgagac cgacgagaag ggccgcgtca
     1081 agctgtccat gaaggcgctg atcgatcgcg atcagcagcc ggccgctggt tcgcaagagt
     1141 gagtcgaacc tgttgaggga cctcgtgctg gccccggacc cttggcggtc cgggttgcct
     1201 gcgcagatcg cctcttgagc acgatgaaag ccattgaaat cagtgcgttc ggcgcccccg
     1261 aaatgctccg actgaccgag cgtccggccc ccattgccgg tccgggagag gtcttgatcg
     1321 ctgtcacggc ctccggtatc aaccggccgg acgtgttgca gcgcaagggc atctatccgc
     1381 cgccggtcgg cgcgtccgat ctgcccgggc tcgaagtcgc gggttcgatc gtgtcgggtg
     1441 atgagcgcgc aatgaccgag gccggactgc gtgtgggcga tcgtgtctgc gcgctggtcg
     1501 ctggcggcgg ctatgccgag ttgtgcgtgg ctcctgtcgg gcagtgcctg ccggtgcctg
     1561 ccgggctgac cgacatcgag gccgccagcc tcccggaaac ctgcttcacc gtttggtcca
     1621 acgtcttcgc acgcgctggc ctgaaggcgg gcgaaacgct gctgatccag ggcggcagca
     1681 gcgggatcgg tgtgactgcg atccagttcg cgcgcgctgc gggcgccacc gtgctggtca
     1741 cggtgggttc agatgaaaag gccgcggcct gcttgaatct tggtgcacac catgcgatca
     1801 attaccgcac cgaggatttc gtcggcgccg tcaagcgctt gacggctggg cgcggcgtcg
     1861 acgtcattct cgacatggtc gccggggact atgtggcacg cgaagtcagc tgcctcgccg
     1921 atgacggtcg ccttgtcatc attgcagtgc agggcggaag cgcggctcag ttcgatgcca
     1981 gccggttgtt gatgcgccgc ctgacaatca ccggctctac gttgcgcgcc cgctccgttg
     2041 aattcaagtc ggcaattgca cgcgagttgc gctcgacggc gtggcgctgg atcgaatcca
     2101 ggctggtcaa ggcggtggtc ttcaggatat tcccggccgc tcaagccgca caggcccacg
     2161 cgctgatgga gtcgaatcag cacatcggaa agttggtgtt gtcatggtga attcacaact
     2221 gtcgaagcca ggcgcaggtc gacgtccgtt ggtggtgggc aactggaaga tgaacggtac
     2281 gcgcgcttcc agtgttcagc tgctgtcggc gttgcggtcc gccgagcctt tcgattcgga
     2341 cgtcagtgtg tgcgtgccgt tcccgtatct gggcgaagta gccctgacgc tgcagggcag
     2401 tcggatcacc tggggcgccc aggattgttc gcatctcgag cctggcgccc ataccgggga
     2461 tgtggcggcg gtgatgctgg ccgatttcgg ttgccgccac gtgatcgtcg ggcactccga
     2521 gcgccgggtt catcatgccg agtcggacca gcgcgttgcc gacaaagcca agactgcgct
     2581 gtcgcatgga ctgacgccga tcgtgtgcgt gggcgaaacg ctggaagaac gccaggcgat
     2641 gcagaccgaa gcggtggtga aacgtcagct gtccgcggtc attcacacgc tggggcattg
     2701 cgtgtcgcag atcgtggtcg cctatgaacc catctgggcc atcggcaccg ggttgaccgc
     2761 gacgccggag caggcgcagg ccgtgcacgc cgtgctgcgc gctcaactgg cggctgccag
     2821 cacgcaggcc gtcgacatgc ggctgctcta cggcggcagc gtcacggctg ccaatgcttc
     2881 cgcgctgttc gcgcagtccg acatcgacgg tggtctggtc ggtggagcat ctctcaaggc
     2941 gaacgaattt tctgccatct gtcgtgcggc ttccgcggcc gtgcatgagt ctcttggaaa
     3001 gtcgaaaaca tgaacctgat gttgaatttg gtggtgatcg tccagatcct tgccgcgctg
     3061 gtgatgatcg gtctggtgct cgtgcagcac ggcaagggcg cagacatggg cgcttcattt
     3121 ggcagtggtg cctcgggcag tctcttcggt gccacaggca gtgcgaattt cctttcgcgt
     3181 tccacggcgg tgtgtgccgc ggtcttcttt gcctgcaccc tcttgctggc cttcctggcc
     3241 aacgaccgat ctcgtgctgc accgaccggc agcgtgctcg accgcccggc gatttctgcg
     3301 cctgacgcag gcgccagcgg cgctacgggc gcgacacaga ttccgggcgg tgcaggcatc
     3361 gcgccagcgc ctgcaactgt ctctgcttca gccgctgcgg cgacctctga cgcgccggca
     3421 tccggcgcgg ctgccatacc ctcgaaatga tgcgctgctg atcgtgcgag tgctgcgatc
     3481 tcgcagcgca aacgctcgga aagcgcgtgc attgaggtta gaatttcggg ctgttaatga
     3541 tcgtatttgc gcaggctgat tcgatgattg acggccgaca gggacacaag gccgacgtgg
     3601 tgaaattggt agacacgcta tcttgagggg gtagtggcga aagct
//
LOCUS       DAOORV010000276         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30337_length_3644_cov_5.899972, whole genome shotgun sequence.
ACCESSION   DAOORV010000276 DAOORV010000000
VERSION     DAOORV010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30337_length_3644_cov_5.899972"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..556
                     /locus_tag="PKV56_19050"
     CDS             185..556
                     /locus_tag="PKV56_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69945.1"
                     /translation="MITSLTIASAAPGWAQALPTRLFPQNALRGEVTFGVPPEVTLNG
                     APARLSPGARLRAQNNMMVLSGTLTGTKQTVHYTLENTGLIKDLWILRDEEKASKPWP
                     TKPEHTSTWSFDPGTQTWTKP"
     gene            553..1896
                     /gene="miaB"
                     /locus_tag="PKV56_19055"
     CDS             553..1896
                     /gene="miaB"
                     /locus_tag="PKV56_19055"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOX69946.1"
                     /translation="MTMPGAGKKVFIKTFGCQMNEYDSDKMVDVLRQAEGYEPTTDVE
                     QADLILFNTCSVREKAQEKVFSDLGRVKHLKSKGVLIGVGGCVASQEGAAIIDRAPYV
                     DVVFGPQTLHRLPELLNRRSTQERAQVDISFPEVEKFDHLPPARVEGGSAFVSIMEGC
                     SKYCSYCVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEITLLGQNVNAYRGAMGRDTA
                     EVADLALLIEYVAEIPGIERIRYTTSHPNEFTQRLIDVYARVPQLVDHLHLPVQHGSD
                     RILMAMKRGYTAMEYKSTVRKLRAVRPRLSLSSDFIVGFPGETDEDFEKMMRLIEDVG
                     FDTSFSFVFSPRPGTPAANLPDDTSQEVKLRRLQRLQAAIEGHAQQISQALVGKRLQI
                     LVEGPSRKDPDELMGRTICNRKVNFKGAPQLVGQLIEVTITQANPNSLRGEAAGT"
     gene            complement(1927..2199)
                     /locus_tag="PKV56_19060"
     CDS             complement(1927..2199)
                     /locus_tag="PKV56_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69947.1"
                     /translation="MNLFDVVWHIANFAAPALLLGAISSVAAKMLWRSQLRGWSWIRL
                     CGVAAAASLIVCVAGLVFSGRDGKMITYAGMVFACAASLWWSTRQR"
     gene            2313..3635
                     /gene="hemA"
                     /locus_tag="PKV56_19065"
     CDS             2313..3635
                     /gene="hemA"
                     /locus_tag="PKV56_19065"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOX69948.1"
                     /translation="MSVFTLGLNHATAPIDLRGRFAFSAEQLAPTLRAFRQRLQRSPE
                     AALVSTCNRTELYVGADAGLVAPAFEWLAEVGGMSGSALREHAYVLENEAAARHAFRV
                     ASGLDSMVLGEPQILGQMKQAVREADTAGTLGTTLHQMFQRSFAVAKEVRTSTEIGSH
                     SISMAAASVRLASQLFEDLTELKVLFVGAGEMIELVATHFAARKPRAMALANRTRERG
                     EKLASKFNAEVLPLSDLPQRLGEFDVVISCTASTLPIIGLGAVERALKARRRRPMFMV
                     DLAVPRDIEPEVSRLSDVYLYTVDDLSSLVQIAGEKRQAAVQQAEAIIETGVQGFVNW
                     LGQRGTVPLIQALNAQADEWRAVEIARARKMLTKGESIEAVLESLSRGLTQKMLHGTL
                     VELNATDVDSRADLTHTVSRLFLRNSGSTTRRQPAAGSNAPDPDPTLL"
BASE COUNT          734 a         1154 c         1148 g          608 t
ORIGIN      
        1 gcgacggaat cataatccgc aggtccgggg ttcgaatccc tgaagcgcca ccagattcaa
       61 gatcggctcc gcgtcttcac gacccggagc cgttttcatc tgtgccctca ccacgaggcc
      121 agggcagaga tcgacaatac gccaccgaca gccactggac gacaccatgt accgctgtat
      181 cgccttgatc acttccctca ccatcgccag cgccgcaccc ggctgggcgc aggctttgcc
      241 gaccaggctg tttccgcaga acgcacttcg cggcgaggtc acattcggcg tgccgcctga
      301 ggtgacgctg aacggtgcgc cggcgcgact gtctccaggc gcacgcctgc gcgcacagaa
      361 caacatgatg gtgctgtctg gcacgctcac gggcaccaag cagactgtcc actacacact
      421 ggagaacacc gggctgatca aggacctgtg gatcctccgc gatgaagaaa aggccagcaa
      481 gccgtggccc acgaaaccgg aacacacgag cacctggagc ttcgatcccg gcacacagac
      541 ctggaccaaa ccatgaccat gcccggcgcc ggcaagaagg tcttcatcaa gaccttcggc
      601 tgccagatga acgagtacga ctccgacaag atggtcgacg tgctgcggca ggccgaaggc
      661 tacgaaccga ctacggacgt cgaacaggcc gacctgatcc tcttcaacac ctgctccgtg
      721 cgcgaaaagg cgcaggaaaa ggtgttcagc gacctgggtc gcgtcaagca cctgaagtcc
      781 aagggcgtgc tgataggcgt gggcggctgc gttgcgagcc aggaaggggc tgcgatcatc
      841 gatcgcgcgc cttacgttga tgtggtgttc gggccgcaga ccctgcaccg gctgcccgag
      901 ttgctgaacc gccgcagcac gcaggagcga gcccaggtcg acatcagttt tccggaagtg
      961 gagaagttcg atcaccttcc acccgcgcgg gtcgagggcg gtagtgcgtt cgtgtcgatc
     1021 atggaaggct gctcgaagta ctgcagctac tgcgtggtgc cttacacccg tggcgaagag
     1081 gtatctcgac cattcgacga tgtgctggtc gaggtcgccg gtctcgccga ccagggcgtc
     1141 aaggagatca cgctgctcgg ccagaacgtc aacgcctatc ggggcgcgat gggccgggat
     1201 accgccgagg tcgccgacct cgccctgctg atcgagtacg tggcagagat cccgggtatc
     1261 gagcgcatcc gttacacgac cagccacccg aacgagttca cgcagcggct gatcgatgtg
     1321 tacgcgcggg tgccgcagct ggtcgaccat ctgcacctgc cagtgcagca tggcagcgac
     1381 cgcatcctga tggcgatgaa gcgcggctac acggcgatgg agtacaagag caccgtgcga
     1441 aaactgcgcg cagtgcggcc ccgtttgagc ctctcgagcg atttcatcgt cggctttccg
     1501 ggcgagaccg acgaagactt cgagaagatg atgaggctca tcgaggacgt gggcttcgac
     1561 accagtttca gcttcgtttt cagcccgaga ccgggcacgc ctgccgccaa cctgccagac
     1621 gacacctcgc aggaggtcaa gcttcgacgc ctgcagcgac tgcaagcggc catcgagggc
     1681 cacgcccagc agatcagtca ggccttggtc gggaaacgtc tgcagatctt ggtcgagggg
     1741 ccttcgcgca aggacccgga cgagttgatg ggccgaacca tctgcaatcg gaaagtcaac
     1801 ttcaagggcg ccccgcaact tgtcggtcag ctgatcgagg tgacgatcac gcaggcaaac
     1861 ccgaattcac ttcgcggtga agccgctgga acctgaccgg gcttgattcc cgagttccag
     1921 cccatatcag cgctgtcggg tgctccacca gagactggca gcgcatgcga acaccatgcc
     1981 ggcgtaggtg atcatcttgc cgtcacgccc ggagaacacg aggcctgcga cacaaacgat
     2041 caggcttgcc gcagccgcca cgccgcacaa ccggatccag gaccagccgc gcaattggct
     2101 gcgccacagc attttggccg ccacggaact gatggctccc agcagcaggg caggtgcagc
     2161 aaaattggcg atgtgccaca cgacatcgaa aaggttcatg gtcgagaagg agcgctgggg
     2221 ttcgatgacg cggcgacaga agcgtcccgg acttatccgg tgttacgcgg tgggctggtt
     2281 tcgcggaatg ctgtaaattt tgcctgacag atatgagcgt attcacactg ggtttgaacc
     2341 acgccaccgc gcccatcgac ctgcgcggac ggttcgcctt ttctgctgaa cagctcgcac
     2401 caacgctgcg ggctttccga cagcgcttgc agcgctcgcc agaagcagcg ctggtgtcga
     2461 cctgcaaccg taccgaactg tacgttggcg ccgacgcggg tctggtggcg ccggcgttcg
     2521 agtggctggc ggaagtcggt gggatgtcgg gatcggcctt gcgcgagcac gcgtacgtgc
     2581 tggagaacga ggccgccgcc cggcatgcat tccgggtggc gagtggcctg gactcgatgg
     2641 tgctgggcga gccgcagatc ctcggacaga tgaagcaggc ggtgcgggag gcggataccg
     2701 caggcacact cggcaccacg ctgcatcaga tgttccagcg atccttcgct gtggcgaagg
     2761 aagtgcgcac ctcgaccgag atcggttcgc attcaatcag catggccgcc gcctcggtgc
     2821 ggctcgcttc acaactcttc gaagacctga ccgaactcaa ggtgctgttc gtcggcgcag
     2881 gagagatgat cgaactggtc gccacgcact tcgccgccag aaaaccccgg gccatggcgc
     2941 tggccaatcg cacccgtgaa cgcggcgaaa agctcgccag caaattcaac gccgaagtgc
     3001 tgccgctgtc cgacctgccc caacgtcttg gcgagttcga cgtggtgatt tcctgcaccg
     3061 cgagcacgtt gccgatcatc ggactgggcg ccgtcgaacg ggcgctgaag gcgcgacggc
     3121 ggcgtccgat gttcatggtc gacctcgcgg tcccgcgcga catcgaaccc gaggtcagcc
     3181 gtttgtctga cgtctacctg tacaccgtgg acgatctctc gtcgctggta cagatcgccg
     3241 gcgaaaagcg gcaggcggct gtgcagcagg ccgaggccat catcgagacc ggcgtccaag
     3301 gcttcgtcaa ctggctgggg caacggggaa cggtgccctt gatccaggcg ctgaacgctc
     3361 aggctgacga gtggcgcgcg gtcgagatcg cacgggcgcg caagatgctg accaaaggcg
     3421 aaagcatcga ggcggtgctc gaatcgctgt cgcgtggcct cacgcaaaag atgctgcatg
     3481 gaaccctggt cgaattgaat gccaccgacg tcgacagccg cgccgacttg acgcacaccg
     3541 tttcgcgcct gttcctgcga aacagcggca gcacgacccg gcggcaacca gcggctggtt
     3601 ccaacgctcc cgatcccgac cctaccttgc tgtagcgcca cgtc
//
LOCUS       DAOORV010000277         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30420_length_3637_cov_5.003350, whole genome shotgun sequence.
ACCESSION   DAOORV010000277 DAOORV010000000
VERSION     DAOORV010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30420_length_3637_cov_5.003350"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKV56_19070"
     CDS             <1..734
                     /locus_tag="PKV56_19070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX69949.1"
                     /translation="DGAVALANGGTLFLDEIGEMPIDLQPHFLRVLEDGAIFRIGENK
                     PRNVSFRLVAATNRDLRKEVAEGRFRMDLFYRIAVATINIPPLRERAEDIQLIADFLL
                     DKLSQQHAVFRPVLDPEALDCLSRHAWPGNIRELRNVLERALLMSSDTVITKESLPHE
                     VRFAETAGVQRLASVPQEGELKRLEQVEKDTIISMIERQSGNMTAVARELGIAKSTLY
                     VKLKKFDIALSGASAIDVSTNGVAA"
     gene            1020..2960
                     /locus_tag="PKV56_19075"
     CDS             1020..2960
                     /locus_tag="PKV56_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOX69950.1"
                     /translation="MGIQINIDNGGTLTDICIIGGERVEKTKVLTTPYDLSKCFFEGL
                     EKASGVVYGKPDVRRLLEEVELIRYSTTQGTNAICERKGPRLGLILDESARNLTSQLA
                     EHDPDVYAALVGDRVVFLSAEVVTGENAQMEVVKAINKLTATGASRIVVSFGGANFAA
                     VEDAFRKVALRKYPRHLLGAVPMLYGSDLSIDKDPVRRTWTALINSFLHPGMEAFLYN
                     AENRLRQFRTKNPLLIFRNDGDASRVAKTIAIKTYSSGPRGGMEGVKSFSKRYGFTDV
                     VSIDVGGTTTDIGQYLDGTVAEVRRGHVEGISVSFPLCEIISAGAGGSSIIKVVNGRI
                     VIGPESVGAVPGPACFGRGGQFATITDASLLAGLFDPKSYFGGGMGLDLDRAATAVTV
                     NVAEPLGLKLEDALQQMELAFEDKIAAELHRVTKVSKDSVLLAFGGAGPLNACGVAEM
                     AGINRVAIPHMAAVFSAYGIGTCDVSQRYTAALASNDQNGLEDALALLRAKASRDMYA
                     EGFDKGDYEIETNLVSTDAGGKETTYPLNVVAPQFPADVGKSALVELEFAAVKRLRAD
                     SSSAAKVIEGGQASSSGVRNILFRGRGRIDVPVYRLAELKAGDHASGPAILEEDYFTC
                     RVLEGWTFVISDAGDIMLNRKA"
     gene            2966..3367
                     /locus_tag="PKV56_19080"
     CDS             2966..3367
                     /locus_tag="PKV56_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HOX69951.1"
                     /translation="MKVLMTEYLRVDLDAEQWECRVCDHVIGTATKSYKEGMLVYDRD
                     PREIHPPILDPERYRFTFSPDPEWVRILEYYCPSCGTQVETEYAIPGHPPLYDMQPDL
                     PALRAQWSKREEVKEGVVGPAVAGGKDGHRH"
     gene            3463..>3637
                     /locus_tag="PKV56_19085"
     CDS             3463..>3637
                     /locus_tag="PKV56_19085"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOX69952.1"
                     /translation="MRRISVDIGGTFTDCFVVWDGKYIEAKALTTHHNLALGFNEALG
                     KANKVLGLELETIL"
BASE COUNT          724 a         1080 c         1131 g          702 t
ORIGIN      
        1 gtgacggtgc cgtcgcgcta gccaatggcg gcacgctttt ccttgatgaa atcggtgaga
       61 tgccgatcga tctgcagccg cacttcctgc gggttctgga agatggggcg atattccgca
      121 tcggcgagaa caaaccgaga aacgtctcgt tccgtctggt tgctgcgact aaccgggatc
      181 tgcgcaagga ggtggccgag ggccgcttcc gcatggacct gttctatcga atcgcggtcg
      241 cgacgatcaa cattccgccg ttgcgcgaac gggccgaaga catccagctg atcgcagatt
      301 tcctgctcga caagctgtcg cagcaacacg ccgtattccg gccggtgctg gatccggagg
      361 cactggattg cctgagtcgc cacgcctggc ccggcaatat tcgcgaactg cgcaatgtgc
      421 tcgagcgtgc attgctgatg tcctcggaca cggtgatcac gaaggagtct ctgccgcacg
      481 aagtccgatt tgccgagacc gcaggagtgc agcggctggc gtccgtacct caagagggtg
      541 aattgaagcg tctggagcaa gtcgagaagg acaccatcat ctcgatgatc gagcgtcaga
      601 gcggcaacat gacagcagtt gctcgggaac tcggcattgc caagagcacg ctatacgtca
      661 agttgaagaa gttcgacatc gcgctgtccg gcgcatctgc catcgacgtg tcgaccaatg
      721 gcgtcgctgc ttgattgctg acagatcttc cggaggtctc catccgacct ctccctgaga
      781 cggcaaccgc gaaccgttgc gaaatccgga cacctcggac agtccggacg tcagggttaa
      841 cgtcaaaggg ctcttcaagc cattgatcta ccgacgattt tttggagtcg tcgactggca
      901 tgtcgcttgc aatccacttg tacgagacat atccatccgc gtatcaagcg aagcacgcat
      961 gagatcgctt tgcgcgcgcc tcgttgcgtg ctcggaacat cacaacctgg gactcgacca
     1021 tgggcataca gatcaacatc gataacggcg ggacgctgac cgacatctgc atcatcggcg
     1081 gtgagcgggt cgagaaaacg aaggtgctca ccaccccgta cgacctcagc aaatgcttct
     1141 tcgaggggct ggagaaggcc tccggagtgg tctacggcaa gcccgatgtg cgtcgcctgc
     1201 tggaggaggt ggagttgatc cgctactcga ccacgcaggg tacgaatgcc atttgcgaac
     1261 gcaagggtcc gcgtctgggc ctcatcctcg acgagagcgc gaggaatctg acttcgcaac
     1321 tcgccgagca tgatcctgac gtctatgctg cgctcgtggg tgaccgcgtg gttttcctga
     1381 gcgccgaggt cgtcactggc gagaacgcgc agatggaggt ggtcaaggcg atcaacaagt
     1441 tgactgccac tggtgccagc cgcatcgtgg tcagcttcgg cggtgccaat ttcgcggctg
     1501 tcgaggacgc attccggaaa gtggctttgc gcaagtaccc tcgccacctg ctgggtgcgg
     1561 tgccgatgct gtatggcagc gacctgagca tcgacaagga cccggttcgt cgcacctgga
     1621 ccgcgttgat caactccttc ctccatcccg ggatggaggc cttcctgtac aacgccgaga
     1681 accggctgcg acagttccgc accaagaacc cgctgctgat cttccgcaat gacggcgatg
     1741 cgtcgcgtgt tgcgaagacc atcgccatca agacctacag ttcaggtccg cggggcggca
     1801 tggagggtgt caagtcgttc tcgaagcgct atggattcac cgatgtcgtc tctatagacg
     1861 tgggtggaac cacaactgac attgggcagt acctcgacgg cacggtggcg gaagtccgcc
     1921 gggggcacgt ggaggggatc agtgtttcgt tccctctttg cgagatcatc agcgcaggtg
     1981 ctggcggtag ctcgatcatc aaggtagtga acggccgtat cgtgatcggg cccgagagtg
     2041 ttggcgccgt gcctgggccg gcttgtttcg ggcgtggtgg ccagttcgcg accatcaccg
     2101 atgccagcct gctggcaggc ctgttcgatc cgaaatcgta tttcggcggt gggatggggc
     2161 ttgaccttga ccgcgcggcg accgccgtga cagtcaatgt cgccgagccg ctgggcctca
     2221 agctcgagga tgcgctgcag cagatggaac tcgcgtttga ggacaagatc gccgccgaac
     2281 tgcatcgcgt caccaaggtc tccaaggaca gcgtcctgct cgccttcggt ggcgccgggc
     2341 cactcaacgc ctgcggagtc gcagagatgg caggcatcaa cagggtcgcc attcctcaca
     2401 tggcagccgt cttcagtgcc tacggcatcg gcacctgcga cgtgtcccag cgctacaccg
     2461 cagccctggc cagcaatgac cagaacggcc tagaggatgc cctcgccctg ctgcgcgcga
     2521 aggcgtcacg cgacatgtac gccgaaggct tcgacaaggg tgactacgag atcgagacca
     2581 atctggtttc aaccgatgcc ggcggcaagg aaacgacgta tccattgaac gttgttgcac
     2641 cccagtttcc cgccgatgtc ggcaagtctg cgctggttga actggaattc gctgccgtca
     2701 agcggctgcg cgcagactcg agcagtgctg caaaggtgat cgaaggtggg caggcgtcct
     2761 cctccggcgt gcgcaacata ttgttccgtg gtcgcggccg catcgacgtg ccggtctacc
     2821 ggctggccga actgaaggcg ggggatcacg ccagtggtcc cgccatcctc gaagaggact
     2881 atttcacctg ccgggttctg gagggttgga ctttcgtcat cagcgacgct ggggacatca
     2941 tgttgaacag aaaggcttga agaccatgaa ggttctgatg accgagtacc ttcgcgtcga
     3001 tctggatgcg gagcaatggg aatgccgggt ctgcgaccac gtcattggca cagccaccaa
     3061 gagctacaag gaaggcatgc tggtctacga ccgtgatccg cgcgagatcc acccgccgat
     3121 cctcgacccg gagcgctatc gcttcacctt cagtcccgat cccgagtggg tccgcatcct
     3181 cgagtactac tgcccgagct gcggcacgca ggtcgagact gaatacgcga ttcccgggca
     3241 cccgccgctc tacgacatgc agcccgacct gcccgcactg cgcgcgcagt ggtccaagcg
     3301 ggaagaggtc aaggaaggcg tggtcggacc ggctgtggcc ggcggcaagg acggtcaccg
     3361 gcactgatcg gcctcaccgt tcgagtgtcg gagagatcgc tgaaccgcga gattccctgc
     3421 gcttgaatcc ggcggctgac gccaccccat caaggagtag aaatgcgacg catatccgtt
     3481 gacatcggtg gcacgttcac cgattgtttt gtggtctggg atggcaagta catcgaggcc
     3541 aaggcgctca ccacccacca caatctggcg cttggcttca atgaggcgct gggcaaggcc
     3601 aacaaggttc tgggccttga actcgagacg atcctgt
//
LOCUS       DAOORV010000278         3619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30628_length_3619_cov_10.716049, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000278 DAOORV010000000
VERSION     DAOORV010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30628_length_3619_cov_10.716049"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..84
                     /locus_tag="PKV56_19090"
     CDS             19..84
                     /locus_tag="PKV56_19090"
                     /inference="COORDINATES: protein motif:HMM:NF014279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69953.1"
                     /translation="MFKKAQVSMFEMTKLVSAHLS"
     gene            complement(185..790)
                     /locus_tag="PKV56_19095"
     CDS             complement(185..790)
                     /locus_tag="PKV56_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOX69954.1"
                     /translation="MSLTIDYYLSPQSPWTYLGHERFCEIARAAKANVRVLPVDLGGK
                     VFPVTGGVPLSKRAPQRQAYRLLELKRFSEALGQPINLQPRYFPVSGDDAARLIVAVD
                     MHDGTEAALALTGAVLRGVWVDDVNTADANVLAAMLATLGLPSRRLDDAYSQAAHARY
                     EADSALAIEAGVFGAPSYVIDGEIFWGQDRLDFVQRRLARG"
     gene            complement(854..2506)
                     /locus_tag="PKV56_19100"
     CDS             complement(854..2506)
                     /locus_tag="PKV56_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOX69955.1"
                     /translation="MSTDTPSTALDKEVRRRRTFAIISHPDAGKTTLTEKLLLFSGAI
                     QIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSED
                     TYRVLTAVDAALMVIDAANGVEPQTRRLLQVCRARNTPILTFVNKMDREVKDPLALMD
                     EIERELGMEVVPFTWPVGMGKQFHGVMDLREKQMRVFSPGEDRDHRNDEILAGLDNPA
                     YAQRFGMQFEQAQGEIELLTDAAPPFDRAEFLAGRQTPMFFGSAVNNFGVREVLDALV
                     DLAPSPGPRAAIQRVVEPGEPKFTGVVFKIQANMDPAHRDRIAFLRVASGHFERGMRL
                     KVVRSGKELRPNTVVSFLSQRRELLDEAFAGDIIGIPNHGVLQLGDTLTEGEALQFTG
                     LPFFAPEMFRSVEVADPLKTKQLKAGLTQLGEEGAIQVFRPVGGTVLLLGAVGQLQFE
                     VVAHRLEHEYGVKARIMPSRFQVARWVTCETGEGAAADERELKRFIDGNSHRVAYDAV
                     DAPTVLVEYAPELRAIEANWPKIRFHALREHAGLVFQKQLDG"
     gene            complement(2553..3176)
                     /locus_tag="PKV56_19105"
     CDS             complement(2553..3176)
                     /locus_tag="PKV56_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOX69956.1"
                     /translation="MRIAIITFDGFNEIDAFVPLGLLNRLTARGWKAEITGPTSHVTS
                     MNGVTVEVQQPLEFANEADAVVFGSGIYTRAIAEDVGRRGALLDRLHLDPLRQMIAAQ
                     CSGSLLMARLGLLADMPACTDSNTKPWVIEAGVRVEDSPFHARGSLATAGGCLASQYL
                     AAWMMARGASLDAAAQALHYAAPVGEKDAYVERVLAVIRPFLPEATG"
     gene            complement(3205..>3619)
                     /locus_tag="PKV56_19110"
     CDS             complement(3205..>3619)
                     /locus_tag="PKV56_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOX69957.1"
                     /translation="LLADTYTLYLTTHNFHWNVTGPMFNTLHQMFMVQYTELWNAVDP
                     VAERIRSLGHAAPGSYAQFGALASIKDAPTTPPKALEMVRILVEGHEAVARTARSIFP
                     LADKAGDEPTADLLTQRLTVHEQTAWMLRSLLETE"
BASE COUNT          571 a         1218 c         1228 g          602 t
ORIGIN      
        1 gacgccaagc tgaaggaaat cttcaagaag gcccaggtct cgatgttcga gatgaccaag
       61 ctggtcagcg ctcacctgag ctgatcgcgg cgcgcaagcg cccctcgatg aagaagccgg
      121 cgcaacccgc cggctttttt ttgcgcgcgt tggaatgccg ggccggccgc gctgcgctgg
      181 ctgcttagcc ccgcgcgagg cggcgctgca cgaaatccag ccgatcctgg ccccagaaga
      241 tctcgccgtc gatcacgtag ctgggcgcac cgaacacgcc ggcttcgatc gccagcgccg
      301 aatcggcttc atagcgggca tgcgcggcct gcgagtacgc atcgtccagc cgccgggacg
      361 gcaggccgag cgtcgcgagc atcgcggcca gcacgttggc atcggcggtg ttcacgtcgt
      421 cgacccacac gccgcgcaac acggctccgg tcagtgccag tgcagcctcg gtcccgtcgt
      481 gcatgtcgac cgcgacgatc agccgcgccg cgtcgtcgcc ggacaccgga aagtacctgg
      541 gctggagatt gatcggttgc ccgagcgcct cgctgaaacg cttcaactcg agcagccggt
      601 aggcctgacg ctgcggtgcg cgtttcgaca gcggcacccc gcccgtcacc ggaaacacct
      661 tgccgccgag atccacgggc agcacgcgca cgttggcttt cgcggcccgg gcgatctcgc
      721 agaagcgctc gtggcccagg taggtccacg gcgactgcgg tgacaggtag tagtcgatgg
      781 tcaggctcat ggcacctccg gcctcaggga ggcgcagtct agccagtcgg tgtgtccgag
      841 tccttgtgtc aggtcaaccg tcgagctgct tctggaacac cagtcccgcg tgctcgcgca
      901 aggcatggaa cctgatcttc ggccagttgg cctcgatcgc acgcagctcg ggcgcgtatt
      961 cgaccagcac cgtcggtgca tcgaccgcgt cgtaggcgac gcggtgcgag ttgccgtcga
     1021 tgaagcgctt gagctcgcgc tcgtcggcag ctgcgccctc gcccgtttcg caggtgaccc
     1081 agcgggccac ctggaagcgg ctcggcatga tgcgcgcctt gacgccgtac tcgtgctcga
     1141 gccggtgcgc cacgacctcg aactgcagct gtccgaccgc gccgagcagc agcaccgttc
     1201 cgcccaccgg ccggaacacc tggatcgcgc cctcctcgcc aagctgcgtc aacccggcct
     1261 tgagctgctt ggtcttcagc gggtcggcca cctcgaccga gcggaacatc tccggcgcga
     1321 agaacggcag cccggtgaac tgcagcgcct cgccctcggt gagggtgtcg ccgagctgca
     1381 gcacgccgtg gttcgggatg ccgatgatgt cgccggcaaa ggcctcgtcg agcagctcgc
     1441 gacgctggct gaggaagctc accacggtgt ttggccgcaa ttccttgccc gagcgcacca
     1501 ccttcagccg catgccgcgc tcgaagtggc ccgaagccac acgcaggaac gcgatgcggt
     1561 cgcggtgcgc cgggtccatg ttcgcctgga tcttgaagac gacaccggtg aacttcggtt
     1621 cgccgggctc gaccacgcgc tggatcgcgg cgcgcgggcc gggcgacggc gccaggtcga
     1681 cgagtgcgtc gagcacctcg cgcacgccga agttgttgac cgccgagccg aagaacatcg
     1741 gcgtctgccg accggcgagg aactcagccc ggtcgaacgg cggtgcggca tcggtcagca
     1801 gctcgatctc gccctgcgcc tgttcgaact gcatgccgaa gcgctgcgca taggccgggt
     1861 tgtcgagtcc ggccaggatc tcgtcgttgc ggtgatcgcg atcctcgccg ggactgaaca
     1921 cgcgcatctg cttctcccgc aggtccatca cgccgtggaa ctgcttgccc atgccgactg
     1981 gccaggtgaa cggcacgacc tccatgccga gctcgcgctc gatctcgtcc atcagggcca
     2041 gcggatcctt gacctcgcgg tccatcttgt tgacgaaggt caggatcggc gtgttgcgcg
     2101 cgcggcagac ctgcagcagc cgccgcgtct gcggctcgac accgttggcc gcgtcgatca
     2161 ccatcagcgc cgcgtcgacc gcggtcagca cgcggtaggt gtcttcggag aagtcctggt
     2221 ggcctggggt gtccagcagg ttgatgacgc agtcgcggta ttccatctgc atcacgctgg
     2281 atgcgacgga gatgccgcgc tgcttctcga tctccatcca gtcggacgtg gcatggcgcg
     2341 acgccttgcg ggccttcacg ctgccggcga tctggatcgc gccggagaac agcaggagct
     2401 tctcggtcag cgtggtcttg ccggcgtcgg ggtgcgaaat gatcgcgaag gtacggcggc
     2461 gccggacttc cttgtcgagc gcggtcgagg gggtatcggt ggacatgggg cgtcgcgcac
     2521 ccggtgggca cgcggctcgt ggaaacctcg aattatccgg tcgcctcggg caggaacggg
     2581 cggatgaccg cgagcacccg ctcgacatag gcatccttct cgccgaccgg cgctgcgtaa
     2641 tgcagcgcct gagcggccgc atcgaggctg gcgccgcgcg ccatcatcca ggctgccagg
     2701 tactgcgagg ccaggcagcc ccccgcggtg gcgagcgagc cgcgcgcgtg gaatggcgag
     2761 tcctcgactc gaacaccggc ctcgatgacc cagggcttgg tgttggagtc ggtgcaggcc
     2821 ggcatgtcgg ccagcagccc cagacgcgcc atcagcaggc tgcccgagca ctgtgcggcg
     2881 atcatctggc gcaacgggtc caggtgcaga cggtcgagca gcgcgccgcg ccggccgaca
     2941 tcctcggcga tcgcgcgggt gtagatcccg ctgccgaaga ccactgcgtc ggcctcgttg
     3001 gcgaactcga ggggctgctg cacctcgacc gtcaccccgt tcatcgacgt gacgtgcgat
     3061 gtcgggccgg tgatctcggc cttccagccc cgcgcggtca ggcggttcag cagccccagc
     3121 ggcacgaacg cgtcgatctc gttgaacccg tcgaaggtga tgatggcgat acgcatgtgg
     3181 ataccccggc tgaaagactc acgtctactc ggtttcgagc aggctgcgca gcatccacgc
     3241 cgtctgttcg tgcaccgtca ggcgctgcgt gagcaggtca gcggtcggtt cgtcgccggc
     3301 cttgtcggcc agaggaaaga tgctgcgcgc ggtgcgggcg accgcttcgt ggccttcgac
     3361 caggatgcgc accatctcca gggccttcgg cggcgtggtc ggcgcatcct tgatcgacgc
     3421 cagtgcgccg aactgcgcat acgagcccgg cgccgcgtgg cccagagagc ggatgcgttc
     3481 ggcgaccggg tcgaccgcgt tccacaactc ggtgtactgg accatgaaca tctggtgcag
     3541 cgtgttgaac atcggaccgg tgacgttcca gtggaagttg tgcgtcgtca ggtacagcgt
     3601 gtaggtgtcg gccagcagg
//
LOCUS       DAOORV010000279         3608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_30748_length_3608_cov_10.478469, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000279 DAOORV010000000
VERSION     DAOORV010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30748_length_3608_cov_10.478469"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PKV56_19115"
     CDS             complement(<1..834)
                     /locus_tag="PKV56_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX69958.1"
                     /translation="MTTIQKRVVRSDLWIDRAFDEGLAGTPDISLAVFPIAGDPSMAW
                     DLLAGAHVYHISAAKDELPQPYFARAELLARCPNLLCVSAGGAGYDTVDVAACTAAGV
                     AVVNQAGGNAASVAEHTLALMLGVSRRMIEGDRRMRREIGYAREDVMGHEIRGHTVGL
                     VGIGHIGRRVAALARAFGMDVIATDPLLSADEIERRGARPVSFEELLAQADFVSLHCP
                     RDKSTLRMMNADAFARMKKGAIFVTTARGGIHDEAALVQALKSGHLAGAGIDVWDQEP
                     PP"
     gene            complement(915..1592)
                     /locus_tag="PKV56_19120"
     CDS             complement(915..1592)
                     /locus_tag="PKV56_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOX69959.1"
                     /translation="MVGLQILERRRQVSAQLVHAFKGLPVANISDCMARMSAAGPRLR
                     PMHKSGHLAGPALTVKCRPGDNLMIHKALTMAQAGDVIVVDAGGDLTNSLFGEIMMAT
                     AVKIGVAGVVLNGAVRDSEEIGQGSFPLYAAGVTHRGPYKDGPGEINVPISIDGMVIH
                     PGDLVVGDADGLLCVPIDDAEAVLAATHQKMDAEKKMLADIAAGTLDTAWIDVALKRI
                     GCNTEPR"
     gene            1734..2411
                     /locus_tag="PKV56_19125"
     CDS             1734..2411
                     /locus_tag="PKV56_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69960.1"
                     /translation="MASAAEKAYEVLKQRVIGGTYAPGAQLKEEHIARELEISRTPVR
                     AALKRLVEDGLAIADPNRGVRVAEWTEYDIEETFDLRGMLEAHAADLAARRGGEALAE
                     RLDELNEQMARAISKGGAVLPERLQHINSLFHRAVLDGSGSPRLRAMLEGLIDMPIVI
                     RSHYISTLQDKQQSLQHHRDLAAAVRAGDGELARQVMQLHLRVAAHRFKRQRLALSTA
                     NASKAPS"
     gene            2408..>3608
                     /locus_tag="PKV56_19130"
     CDS             2408..>3608
                     /locus_tag="PKV56_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOX69961.1"
                     /translation="MIPALDPAAKPASLAIEFLAELRLRGFEGDMAQTHAERTVFATD
                     NSIYQLAPQAVLFPRHELDVVRIAKLSAESRFSTLTFAPRGGGTGTNGQSLTDGLCVD
                     LSRHMNAILEVNAEEGWARVQAGVVKDQLNDAVAKHGLFFAPELSPSNRATIGGMIAT
                     DASGQGSVLYGKTRDHVQELRAVFMDGTEWLSRPLSALELATAKGRTDRVGHVLRVLD
                     RIRQEEGELIAERFPKLNRCVTGYDLVHLCDCHGRFDLSAVLCGAEGTLAFVTEAKVR
                     LTPVPRCTALLNIRYASFDAALRDAGTLMKLQAASSETIDATVLALARKDAIWLKVRD
                     YFPDDPEGPAEGVNIVEFVAAEPAALEAQLARVQAALAAGGAGRRGYTVARGADAAAA
                     IWTMRKKS"
BASE COUNT          605 a         1212 c         1200 g          591 t
ORIGIN      
        1 cggtggcggc tcctggtccc agacatcgat gcccgcgccg gccagatggc ccgacttcaa
       61 cgcctgcacg agagctgctt cgtcgtggat accgccacgc gccgtggtga cgaagatcgc
      121 gcccttcttc atcctcgcga aggcgtcggc gttcatcatc ctcagggtgc tcttgtcgcg
      181 cgggcagtgc agcgacacga agtccgcctg cgccagcaac tcttcgaacg ataccggcct
      241 ggcgccgcgc cgctcgatct cgtcggcgga gaggagcgga tcggtggcga tcacgtccat
      301 gccgaacgcg cgggccagcg ccgcaacgcg cctgccgatg tggccgatgc cgaccaggcc
      361 aacggtatgg ccgcggatct cgtgacccat cacgtcctcg cgggcgtaac cgatctcgcg
      421 gcgcatgcgg cgatcacctt cgatcatcct tcgcgagaca cccagcatca gcgccagcgt
      481 gtgttccgcg accgaggctg cgttcccgcc ggcctggttg acgacggcca cgcccgctgc
      541 cgtgcaggcg gccacgtcca cggtgtcgta gcccgcacca cctgccgaca cgcacagcag
      601 gttcgggcac cgcgcgagca gttcggctcg ggcgaagtag ggctgcggca gttcgtcctt
      661 ggctgccgag atgtgataga catgcgcccc ggccagcagg tcccaggcca tggagggatc
      721 gcctgcgatg gggaagacgg ccagcgagat gtccggtgta cctgccaagc cctcgtcgaa
      781 ggcgcggtcg atccaaaggt ccgagcggac cacccgcttc tggatggtgg tcatctcagt
      841 accgcccggc cgttccgaag gtactgagcg ccccctcggg gggcagcgag cgcagcgagc
      901 gtgggggctt catttcagcg gggttccgtg ttgcagccga tgcgcttgag cgcgacgtcg
      961 atccaggccg tgtcgagcgt gcccgccgcg atgtccgcga gcatcttctt ctcggcatcc
     1021 atcttctggt gtgtcgcagc cagcacggct tcggcgtcgt cgatgggcac gcacagcagg
     1081 ccatccgcat cgccgacgac caggtcgcca ggatggatca ccatgccgtc gatcgagatc
     1141 gggacgttga tctctccggg gccatccttg tacggcccac gatgggttac acctgcggcg
     1201 tagagcggga agctgccttg gccgatctct tcggagtccc gcacggcgcc attcagcacc
     1261 acgccggcca ccccgatctt gaccgccgtg gccatcatta tttcgccgaa cagcgagttg
     1321 gtgaggtcgc caccggcatc gaccacgatc acgtcgcctg cctgcgccat ggtgagtgcc
     1381 ttgtggatca tcaggttgtc gccggggcgg cacttcacgg tgagcgcagg cccggcgaga
     1441 tggccgctct tgtgcatggg acgcaggcgc ggcccagcgg cggacatccg cgccatgcaa
     1501 tcgctgatgt tggccaccgg caagcccttg aaggcatgga cgagttgcgc ggagacttgt
     1561 cgcctgcgct ctaggatctg gagaccaacc atgttgtgtt cgccttggag ttgaacttga
     1621 tggagtgcaa tgtacactaa aaaaacaatc ggtcaagtga tctcgcatga gagcgtttgt
     1681 ctaaggcggg acttgcccgt caactcgcgc tacaagtaca acctaggaac aaaatggctt
     1741 ctgccgccga aaaggcttac gaggttctga agcagcgggt gatcggcggc acttacgccc
     1801 ccggcgccca gctgaaggag gaacacatcg cgcgcgagct ggagatcagc cgcacgccgg
     1861 tgcgggccgc gctgaaacgc ctggtggagg acggactggc cattgccgac ccgaaccgcg
     1921 gggtgcgcgt ggccgagtgg accgagtacg acatcgaaga gaccttcgac ctgcgcggca
     1981 tgctcgaagc ccatgccgcc gatctggcgg cgcgccgcgg gggtgaggca ctggctgaac
     2041 ggttggatga actgaacgag cagatggccc gcgccatcag caaaggcggc gccgtattgc
     2101 ccgagcggct gcagcacatc aactcactgt tccatcgggc cgtgctggat gggagcggct
     2161 cgccgcgcct gcgcgccatg ctcgaaggtc tcatcgacat gcccatcgtg atccgctcgc
     2221 actacatcag caccctgcag gacaagcagc aaagccttca gcaccaccgt gatctggccg
     2281 ccgccgtgcg cgccggcgac ggtgagctgg cccgccaggt gatgcagttg cacttgcgcg
     2341 tcgcggcgca ccggttcaaa cgccagcgcc tggcgctgag cacggcgaac gcatcgaagg
     2401 ctccttcgtg atcccggcac tcgaccctgc ggccaagccc gcttcgcttg ccatcgagtt
     2461 cctggccgag ctgcggctgc gcggcttcga gggcgacatg gcgcagacgc acgccgaacg
     2521 cactgtcttt gccaccgata actcgatcta ccagctggca ccgcaggctg ttctctttcc
     2581 gcgccacgag ctcgacgtgg tgcgcatcgc caagctgagc gcggagtcgc gcttcagcac
     2641 cttgacgttc gcgccacgcg gcggcggcac gggcaccaac ggccaatcac tcaccgacgg
     2701 cctgtgcgtg gacctgtcgc gccacatgaa cgccatcctc gaggtcaatg cagaggaggg
     2761 ctgggctcgc gtgcaggccg gcgtggtgaa ggaccagctc aacgatgcgg tggcgaagca
     2821 tgggctcttc ttcgcccccg aactttcgcc ttcgaaccgc gccaccatcg gcggcatgat
     2881 cgccacggac gcctcgggcc agggctcggt gctctacggc aagacgcgcg accacgtgca
     2941 ggaactgcgc gcggtgttca tggacggcac cgagtggctc tcgcgaccgc tgagtgcgct
     3001 ggagctggcc accgccaagg ggcgcacgga ccgcgtcggc catgtgctgc gcgtgctgga
     3061 ccggatccgg caggaagaag gcgagctgat cgccgagcgg ttcccgaagc tgaaccgctg
     3121 cgtcacgggc tacgacctcg ttcacctgtg cgactgtcac ggccggttcg acctgagcgc
     3181 cgtgctgtgc ggcgccgaag gcacgctcgc ctttgtcacc gaggcgaagg tgcggctgac
     3241 gccggttccg cgttgcacgg cgctgctcaa catccgctac gccagcttcg atgcggcgct
     3301 gcgcgatgcc ggcaccttga tgaagttgca ggccgcctcc agcgagacca tcgacgccac
     3361 cgtgctcgcg ctcgctcgaa aggacgccat ctggctcaag gtgcgcgatt actttcccga
     3421 cgacccagaa ggcccagcgg aaggcgtcaa catcgtcgag ttcgtggcgg ccgagcctgc
     3481 ggcgctggag gcccagctgg cgcgtgtcca agcggcgctg gcggccggtg gcgcgggtcg
     3541 caggggctac accgtcgccc gcggcgccga tgccgcggcg gccatctgga cgatgcgcaa
     3601 gaagtcgg
//
LOCUS       DAOORV010000280         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_31702_length_3528_cov_5.230636, whole genome shotgun sequence.
ACCESSION   DAOORV010000280 DAOORV010000000
VERSION     DAOORV010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31702_length_3528_cov_5.230636"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKV56_19135"
     CDS             <1..297
                     /locus_tag="PKV56_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOX69962.1"
                     /translation="ACGDVMKLQIKVNPATGLIEDARFKTYGCGSAIASSSLVTEWVK
                     GKTLDQAMTIKNTQIAEELALPPVKIHCSILAEDAIKAAVLDYKTKHGSAAESH"
     gene            308..631
                     /gene="iscA"
                     /locus_tag="PKV56_19140"
     CDS             308..631
                     /gene="iscA"
                     /locus_tag="PKV56_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOX69963.1"
                     /translation="MAVTLTEAAARHVTRYLGRRGKGVGVRLGVKTTGCSGLAYKLEY
                     ADDVAPEDIIFEDHGVKVLVDPKSLPYIDGTELDFVREGLNEGFKFHNPREKDRCGCG
                     ESFRV"
     gene            778..1296
                     /gene="hscB"
                     /locus_tag="PKV56_19145"
     CDS             778..1296
                     /gene="hscB"
                     /locus_tag="PKV56_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOX69964.1"
                     /translation="MKIDSDDFELFGVPRRFEQDAAALDARWRTLLSEAHPDRFVSEG
                     ASAQRLAMQWAVRINEAHRRLKDPLRRAAYLCELQGVPINAEDNTAMPGSFLMQQMEW
                     RESLDAASRLAEVEALASEVASHRDCALSELRALIDDRQDPVAAAQQVRALMFVERFA
                     RDVDARLEALEQ"
     gene            1301..3172
                     /gene="hscA"
                     /locus_tag="PKV56_19150"
     CDS             1301..3172
                     /gene="hscA"
                     /locus_tag="PKV56_19150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOX69965.1"
                     /translation="MALLQISEPGGAPDPHQRRIAVGIDLGTTHSLVASVRHGVAECL
                     PDGAGRVILPSAVRYLANGRRQIGAEALAEAATDPRNTLVSVKRFMGRGLADIVDRAK
                     LPYDFVEAPGMLQIRTVDGVKSPVEVSAEILATLRQRAEDTFDDELFGAVVTVPAYFD
                     DAQRQATKDAAQLAGLNVLRLINEPTAAAIAYGLENGSEGLYAVYDLGGGTFDVSLLR
                     LSAGVFEVVATGGDSALGGDDFDHALADWAQAQAGLEVRSAQDKRAVLVAARAAKEKL
                     SLADHATLASPVSASDGSSTELSVRVTRDQFNAITHDLVARTLAVLRKVLRDARTDKS
                     AVQGVVMVGGSTRMPAVQAAVADFFGKAPLNNLNPDEVVALGAALHANTLAGNAGAEE
                     MLLLDVIPLSLGLETMGGLVERIIPRNSSIPTARAQDFTTFKDGQTALAIHVVQGERE
                     LVSDCRSLARFELRGIPPMVAGAARIRVSFTVDADGMLSVAAREEGSGVEASIAVKPS
                     YGLADEDIARMLSESFTTAEVDMKERALRESRVEAERMLLATRAAIDADGDLLEVPES
                     AAIESMLQRLEAVRQGSDHHAIDDAVEALALSTEAFAAARMNRGIRKALAGRRVTEV"
     gene            3187..3525
                     /gene="fdx"
                     /locus_tag="PKV56_19155"
     CDS             3187..3525
                     /gene="fdx"
                     /locus_tag="PKV56_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOX69966.1"
                     /translation="MPIIRILPHAEYCPHGTEIKAVAGTSICEALLENGIEIEHACEM
                     SCACTTCHVVVRQGFASLGEMDEVEEDLLDRAWGLEPNSRLSCQAILSQQDVTIEIPK
                     YTINHAKEQH"
BASE COUNT          622 a         1112 c         1214 g          580 t
ORIGIN      
        1 gcctgcggcg acgtgatgaa gctgcagatc aaggtcaacc cggccaccgg gctgatcgag
       61 gacgcgcgct tcaagaccta cggctgcggc tcggccatcg cgtcgagctc gctggtcacc
      121 gaatgggtca agggcaagac gctggaccag gcgatgacga tcaagaacac ccagatcgcc
      181 gaagagctgg cgctgccgcc ggtgaagatc cactgcagca tcctggccga agatgccatc
      241 aaggcggctg tgctggacta caagaccaag cacggcagcg ccgcggaatc acactgaagt
      301 tggccccatg gcagtcaccc tgaccgaagc cgccgcgcgg cacgtcacac gctacctggg
      361 ccgccgcggc aagggcgtgg gcgtgcgcct gggcgtgaag accacgggct gttccggcct
      421 ggcctacaag ctggaatacg cggacgacgt ggctcccgag gacatcatct tcgaggacca
      481 cggcgtgaag gtgctggtgg acccaaagag cctgccctac atcgacggca ccgaactcga
      541 cttcgtgcgc gagggcctga acgaaggctt caagttccac aacccgcgcg agaaggaccg
      601 ctgcgggtgt ggcgagtcgt tccgggtgtg agtggctttc tgctgcgcgg tccgatctgg
      661 cgtctgcggt gctcaccgta cctgggtacg gctccgctcc tcaacaccac ctcgacccgc
      721 tcgcgacgaa atcctctcgc cctctgagac accggtgcat tcggaccggg cgtggcgatg
      781 aagatcgaca gcgacgactt tgaactcttc ggtgtgcccc gccgatttga acaggatgct
      841 gctgcgcttg atgcccgctg gcgtaccttg ctgagcgaag cgcatcccga tcgtttcgtc
      901 agcgaaggtg cctcggcgca gcgcctggca atgcagtggg cggtgcggat caacgaggcg
      961 catcgccgcc tgaaagaccc gctgcgccgt gcggcctacc tgtgcgaact gcaaggcgtg
     1021 cccatcaatg ccgaagacaa caccgcgatg cccggctctt tcctgatgca gcagatggaa
     1081 tggcgcgagt cgctcgacgc cgcgtcgagg ctggccgaag tcgaggcgct ggcatcggag
     1141 gtggcgagtc atcgtgactg cgccttgagc gagttgcgcg cgctgatcga tgatcggcag
     1201 gaccctgtcg cggcagcgca gcaggtcaga gccctgatgt tcgtcgagcg ctttgcccgt
     1261 gatgtcgatg cgcgtctgga agcgctggaa caatagcgac atggctcttc tacagatttc
     1321 agaacccggc ggagcgccgg acccgcacca gcgccgcatc gcggtcggca tcgacctcgg
     1381 taccacgcac tcgctggtcg cctcggtccg tcacggtgtc gccgaatgcc tgcccgatgg
     1441 ggccgggcgt gtgatccttc cttcagccgt tcgctacctc gccaacggtc gacgccagat
     1501 cggtgccgaa gcgctggccg aggcggccac cgatccgcgc aacacgctgg tatcggtcaa
     1561 gcgtttcatg gggcgcggtc tcgccgacat cgtcgatcgc gccaagctgc cttacgactt
     1621 cgtcgaggcg ccgggcatgc tgcagatccg cactgtcgat ggagtcaagt cgcctgtcga
     1681 ggtttcggcc gagatcctcg ccaccctgcg gcagcgcgcc gaggacacgt tcgacgacga
     1741 gctgttcggt gcggtggtca ctgtgccagc ctacttcgac gatgcgcagc gtcaggccac
     1801 gaaggacgct gcgcaacttg ccggtctgaa tgtgctgcgg ctgatcaacg agccaacggc
     1861 tgcggccatc gcctacgggc tggagaacgg ctccgagggt ctgtatgccg tctacgacct
     1921 gggcggcggc accttcgacg tctcgctgct gcgcctgagc gcgggcgtgt tcgaggtggt
     1981 cgcgaccggt ggcgattcgg ccctgggcgg cgatgacttc gatcatgcgc tggcagactg
     2041 ggcgcaggcg caggccgggc tcgaggtcag gtcggctcag gacaagcgag ccgtgctggt
     2101 ggccgcccga gcggccaagg agaagctctc gttggccgat catgcgacgc tggcaagccc
     2161 ggtgtccgcc agcgacggtt catcgaccga gctgtcggtc cgcgtcacgc gcgaccagtt
     2221 caatgcgatc acgcacgacc tggtggcacg cacactggcg gttctgcgca aggtgctgcg
     2281 cgatgcaagg acagacaagt cggcggtcca gggtgtcgtg atggtcggtg ggtcgacgcg
     2341 catgccggcg gtgcaggctg cggtggccga cttcttcggc aaggcgccgc tgaacaacct
     2401 gaatccggat gaggtggttg cgctgggtgc cgcgctgcac gcgaacacgc tggccggcaa
     2461 tgccggtgcc gaggaaatgc tgctgctcga cgtgattccg ctgtcgctgg gcctggagac
     2521 catgggtggg ctcgtcgagc gcatcattcc gcgcaacagt tcgatcccga ccgctcgtgc
     2581 acaggacttc accaccttca aggacgggca gaccgcgctg gcgatccacg tggtgcaggg
     2641 cgagcgcgaa ctggtcagcg actgcaggtc gctggcgcgc ttcgaactgc gcggcattcc
     2701 gccgatggtg gcgggcgcgg cacgcatccg tgtcagcttc accgtcgatg ccgacggcat
     2761 gctcagtgtg gcggcgcgcg aagagggttc gggtgtcgag gcgtcgatcg ccgtgaagcc
     2821 gagctacggt ctggccgacg aggacatcgc gcgcatgttg agcgaaagct tcacgaccgc
     2881 cgaagtcgac atgaaggagc gtgcgctgcg cgagtcgcgc gtcgaggccg agcgcatgtt
     2941 gctggcgaca cgcgcggcga tcgacgccga tggcgacctg ctcgaagtgc cggaatcggc
     3001 ggcgatcgag tcgatgcttc agcggctgga ggcggtgagg cagggcagtg atcaccacgc
     3061 gatcgacgat gcggtcgaag cgctggcgct gagcaccgaa gcctttgcag ccgcgcgcat
     3121 gaaccgaggc atccgcaagg cgctggcggg acgccgcgtc accgaggtct gaacgaggaa
     3181 ccccgcatgc ccatcatcag gatcctcccc cacgcagagt attgccccca cggcaccgaa
     3241 atcaaggccg tggccggaac gtcgatctgc gaggcgttgc tcgagaacgg catcgagatc
     3301 gagcatgcct gcgagatgag ctgtgcctgc acgacctgcc acgtggtggt caggcaggga
     3361 tttgcgtcgc tcggcgagat ggacgaggtc gaggaagacc tgctcgatcg cgcctggggc
     3421 ctggagccca actcgcgtct gtcgtgccag gcgatcctgt ctcagcagga cgtgacgatc
     3481 gagataccga agtacacgat caatcacgcc aaggaacagc actgagga
//
LOCUS       DAOORV010000281         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_31822_length_3518_cov_8.861681, whole genome shotgun sequence.
ACCESSION   DAOORV010000281 DAOORV010000000
VERSION     DAOORV010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31822_length_3518_cov_8.861681"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKV56_19160"
     CDS             complement(<1..427)
                     /locus_tag="PKV56_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOX69967.1"
                     /translation="MTPLRQAAADYLMLRRALGFKLRANEDALTEFTDFLDRRGATTI
                     TAAAAVEWALSKPSARPGFAADRLRRIRGFTRHHLLSDPATEVAPANLLPSHRHRRQP
                     YLYSDNELARLMTCALRLPPVDSLRGATYHCLLGLLSVTG"
     gene            complement(424..1347)
                     /locus_tag="PKV56_19165"
     CDS             complement(424..1347)
                     /locus_tag="PKV56_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX69968.1"
                     /translation="MPSTERTPTAAEQIEAEYVDYMRHERNLAPATIRSNRIEARNLL
                     TSLFADGPLTFDTVAARDVMAHVRRATKACRPNSACRVVGGVRAFLRFALYRGWLDID
                     LSSHIPAPAVWSQSSIPRALDDDQVARTLAQCDRATPNGCRDYAVILLLARLGLRAGE
                     VVALQLGDIDWRTGELQVRNGQTRVDRLPLPHEVGQALAQYLSEARPRCSSREVFLRA
                     QAPLEGFSSGTAIAAIVRRALQRAGIDAPSKGAHVLRHTLATRLLREGASLPEIGEVL
                     RHRQQQTTTIYAKVDLISLRAIAPAWPGGVQ"
     gene            complement(1377..2459)
                     /locus_tag="PKV56_19170"
     CDS             complement(1377..2459)
                     /locus_tag="PKV56_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOX69969.1"
                     /translation="MARPKATLELSEGERTELQALARRRKSAQAAALRARIVLACAEG
                     VENNMVAERLHVTKQTVSKWRGRFVRERLDGLLDAPRSGAPRTIDDARVEAVVTRTLE
                     SKPAGATQWSTRLMAREMGMTQNAILRIWHAFGLQPHRQETFKLSADPMFVDKVRDIV
                     GLYLNPPLMGMVLCVDEKSQIQALDRTQPMLPLAPGLPERRTHDYVRHGTTTLFAALD
                     VATGRVIGQTQRRHRSAEFLKFLRTVEANVPEVLDIHLVMDNYGTHKTDAIKRWLAAR
                     PRFHVHFTPTSASWLNQVERWFSTLTERCIRRGTHRSTQALEKSIRQYVDLNNEDPKP
                     FVWTKTADDILASIERFCLRTSQSGH"
     gene            complement(2535..2825)
                     /locus_tag="PKV56_19175"
     CDS             complement(2535..2825)
                     /locus_tag="PKV56_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69970.1"
                     /translation="MEQLFARPRAQMRMHDGPLGGYIDACIDSLAAKGYSRDSRRRSA
                     WLVGDFSRWLDRRGIAAAAVNQGVVDAFLHGRKRRRTPRVEDRSTLLRLLVS"
     gene            complement(2909..>3518)
                     /locus_tag="PKV56_19180"
     CDS             complement(2909..>3518)
                     /locus_tag="PKV56_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69971.1"
                     /translation="VITQSTNGKGELREDFDIIVHDGLRDESKSLKLVSGGERVWISC
                     RSRHEIHYAESPDMPSSHSGLRASPCNCAAGTCEAMRHNTSGPAAISANARGGKSLSL
                     MSGGERTFIDACLTRAIALYLAQNTGRRFDTLFSDEADGPLDPEHKRMFMAMKREVLR
                     LGGYRQEFFITQTPHLAAMADAIIDLDAMQVDARAGANAALG"
BASE COUNT          613 a         1136 c         1145 g          624 t
ORIGIN      
        1 tcccggtgac gctgagcagc cccaagaggc agtggtaggt cgcccctcgc aggctgtcga
       61 cgggaggtag cctcaatgcg cacgtcatca accgagcgag ctcattgtcc gagtacaggt
      121 atggttgtcg ccgatgccga tgcgaaggca ggaggttggc gggcgctacc tccgtggcag
      181 gatcggacaa caggtgatgg cgcgtgaacc cgcgaatgcg ccgcagccga tcggcggcga
      241 aaccaggacg tgcggacggc ttgctcaagg cccactccac ggcagcggct gccgtgatgg
      301 tcgtggcgcc gcgccggtcg agaaagtccg tgaactcagt cagcgcgtct tcgttcgctc
      361 gaagcttgaa gccgagcgct cggcggagca tcaggtagtc ggcggcggcc tgtctcagcg
      421 gcgtcattgc acacctcccg gccaagcagg agcaatggct cgcagggaga tcaagtcaac
      481 cttcgcatag atcgtcgtag tctgctgctg gcggtgacgc aacacctcac cgatctcggg
      541 caaagacgcc ccctcgcgca gcagtcgtgt ggccagcgtg tgacgcagca catgcgctcc
      601 cttgctcggc gcatcgatgc cggcgcgctg aagcgcgcgg cgaacgatcg ctgcaatagc
      661 ggtgccgctg gagaaccctt ccagtggtgc ctgtgctcgg aggaagactt ccctggagga
      721 acagcgtggc cgagcctcgc tcaaatactg cgccagcgct tggcccactt cgtgtggaag
      781 cgggaggcga tcgactcggg tttgcccatt gcgaacctgg agctcgccgg ttcgccagtc
      841 gatgtccccc agttgtagag ctaccacctc gccagcccgc agcccgagcc gcgcaaggag
      901 gaggatgacc gcgtagtctc gacacccatt cggtgtagcc cgatcacatt gagcgagggt
      961 gcgcgccacc tgatcgtcat cgagcgcgcg cgggatcgac gactgcgacc aaacggcagg
     1021 cgccggtatg tgcgaagaca aatcgatgtc gagccaaccg cggtagagcg cgaaccgcaa
     1081 gaacgcccga actccaccga cgactcggca tgcagagttc ggacggcacg ccttcgtcgc
     1141 ccgcctcaca tgggccatga cgtcccgcgc cgcaacggtg tcgaacgtca aaggcccatc
     1201 ggcgaagagg gacgtcaaca ggttcctcgc ctcgatcctg ttggagcgga tggttgcagg
     1261 cgccagattg cgttcgtggc gcatgtagtc aacatactcg gcctcgatct gctcggccgc
     1321 ggttggagtg cgctcagttg agggcatggt gaggacgcct tccgacacga gatgcgctag
     1381 tgtcctgatt gagaagttcg cagacaaaac cgctcgatgc tcgcgaggat gtcgtcggcc
     1441 gtcttggtcc aaacgaacgg cttgggatcc tcgttattga ggtcgacgta ttgcctgatg
     1501 gacttctcca gcgcctgcgt ggagcggtgc gtgccgcggc ggatgcagcg ctcggtgagt
     1561 gtggagaacc agcgctcgac ctggttgagc catgacgccg aggtcggcgt gaagtgcacg
     1621 tggaagcgcg gtcgtgcggc cagccagcgc ttgatggcgt cggtcttgtg cgtgccgtag
     1681 ttgtccatga ccaagtggat gtccagcact tccgggacgt tggcctcaac cgtgcgcagg
     1741 aacttcagga actcggcgct gcggtgccgg cgctgggtct gtccgatgac ccggccggtg
     1801 gccacgtcca gcgcggcgaa cagcgtggtc gtgccgtggc gaacgtagtc gtgcgtgcgc
     1861 cgctcgggca agcccggcgc cagaggcagc atgggctgcg tacggtccag cgcctggatc
     1921 tggctcttct cgtccacgca cagcaccatg cccatcagcg gcgggttcag gtacagcccg
     1981 acgatgtcgc gcaccttgtc cacgaacatc ggatcggccg agagcttgaa cgtctcctgg
     2041 cggtgcggct gcagtccaaa cgcgtgccag atgcgaagga tcgcgttctg ggtcataccc
     2101 atctcgcggg ccatcagccg cgtgctccac tgcgtagcgc cggccggctt ggactcgagc
     2161 gttctcgtga ccaccgcttc gacgcgcgca tcatcgatcg tgcgcggtgc tcccgatcga
     2221 ggcgcgtcaa gcaggccgtc gagccgttcg cgtacgaagc gcccgcgcca cttcgagacg
     2281 gtctgcttgg tcacgtgcaa ccgctcggca accatgttgt tctcgacgcc ctcggcgcag
     2341 gccagcacga tgcgggcacg cagcgcagca gcctgggcac tcttgcgccg ccgcgccagc
     2401 gcctgcagct cggtgcgctc gccctcgctc aactccagcg tcgccttcgg tcgcgccatc
     2461 ctcgaacctc gtcgtgcacc acagggtaca cgttcagatt cgtggcatag cagatagttc
     2521 aacgaacttc tcaatcagga cactagcagc cgcagcagcg tagagcggtc ttcgaccctc
     2581 ggcgtgcgac gacgcttacg gccgtgcaag aaggcatcga ccacgccttg gtttactgca
     2641 gcggcggcga tgccgcgtcg atccaaccag cggctgaagt ccccaaccaa ccacgccgag
     2701 cggcgtcgcg agtcgcggct gtagcctttg gcggccagcg agtcgatgca ggcatcgatg
     2761 taaccgccca gcgggccatc gtgcatccgc atctgcgccc gggggcgcgc gaacaactgt
     2821 tccatgagga tctccttcag ggaaaaccgt tcccggcaaa gtcgaaccta tggcagtccg
     2881 gcgctcctgt gaacccgagt agttcgcgtc atccgagcgc agcattcgcg cccgctcgcg
     2941 catcgacctg catggcatcc aggtcgatga ttgcatcggc catcgctgcg aggtgcgggg
     3001 tctgggtgat gaagaactcc tgccggtagc cgccgagccg aaggacttcg cgcttcatgg
     3061 ccatgaacat gcgcttgtgt tctgggtcga ggggcccatc ggcctcgtcg gagaagagcg
     3121 tgtcgaatcg ccgtccggtg ttctgggcca agtacagggc gatcgcgcgc gtcaggcagg
     3181 cgtcgatgaa cgttcgctca ccgccgctca tcaggctgag gctcttgcct ccgcgcgcgt
     3241 tggcgctgat cgctgccggc cccgacgtgt tatgccgcat cgcttcgcag gtacccgcag
     3301 cacagttgca ggggctggca cgcaggccag aatgcgaact cggcatatcc gggctctcgg
     3361 catagtggat ctcatgccgt gatcggcatg agatccagac ccgctcgccg ccgctgacga
     3421 gcttcaagct cttcgattcg tcccgcaacc catcgtgcac gatgatgtcg aagtcctcgc
     3481 gaagctcgcc cttcccgttg gtcgactgcg tgatcacc
//
LOCUS       DAOORV010000282         3517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_31833_length_3517_cov_13.116407, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000282 DAOORV010000000
VERSION     DAOORV010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31833_length_3517_cov_13.116407"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..816)
                     /locus_tag="PKV56_19185"
     CDS             complement(73..816)
                     /locus_tag="PKV56_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69972.1"
                     /translation="MNTKRPTPAQRRRLAQTPDWSPQGVGLAPELYAAALRYLFDRPV
                     PQAQEQEWFWNIDEPDFEATPLEWTRIQTVLFAQAGTDLAAYSDEQVGMGLNFVMSNA
                     VSDVPFAAIDASVPLGEAMRMMQAMPALWRQCIGPRLAPMRLPIGESAGRLAFVCYMW
                     FDVWPTFWNVRHEPRWRDAVWQVLREMLDVPCREVQVAALHGIGHCGRHLERQEAIDR
                     TVEAYILSVDKNDRELKNYADAARRGCVQ"
     gene            complement(1119..2255)
                     /locus_tag="PKV56_19190"
     CDS             complement(1119..2255)
                     /locus_tag="PKV56_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOX69973.1"
                     /translation="MKIKRVTVKKNERGLLLRNGDFERVLQPGTHWLFAGLDTLRVEL
                     FALEQPAFTHGLADYLMAKEPEVVAAEFVRVELTEHQVGLRTENGALVEVLAPATRRL
                     YWKGLVDVQVEVIDIGESAEVPPALVARLVQTQLRQRAVAGLAGVLQVQVPEHGAGML
                     WIDGKVERLLAAGSYAFWKFNRNVSVELVDLRLQALEVTGQEILTRDKVALRLNLSAT
                     WRFTDVLKAYKELAKPADHLYRELQFGLRAAVGTRSLDELLENKTVIDEVVTAQVKAK
                     LADYGLQLDGVGVKDIVLPGEMKTILAQVVEAGKSAEANVIRRREETAATRSLLNTAK
                     VMEDNPVALRLKELETLERVAERIDRISVFGGLDQVLNGLVKLR"
     gene            complement(2498..2571)
                     /locus_tag="PKV56_19195"
     tRNA            complement(2498..2571)
                     /locus_tag="PKV56_19195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2537..2539),aa:Met,seq:cat)
     gene            complement(2764..3339)
                     /locus_tag="PKV56_19200"
     CDS             complement(2764..3339)
                     /locus_tag="PKV56_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOX69974.1"
                     /translation="MHHQHHSHVLQLDIQGTPQAWISLEHAAVHVATGSVAWVDGCGP
                     LATLRGGFNVVRGRQSVIDVFPIMALRGASRVNLFDVVPAFSKLKLFRRDRMTCAYCG
                     QRFHERDLQCEHILPESRGGRWAWMNLVTACGACNGRKADRTPEEAGMPLVYLPYVPS
                     RFEDFLLEGRHIRADVHEWLAARLPKGSRLS"
     gene            complement(3384..3455)
                     /locus_tag="PKV56_19205"
     tRNA            complement(3384..3455)
                     /locus_tag="PKV56_19205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:Arg,seq:ccg)
BASE COUNT          546 a         1195 c         1142 g          634 t
ORIGIN      
        1 catcttcagc ggcacgccgc cgggcacgtc ttcgtggatg tggttggttt ccatgttgat
       61 gtccttctcg cgtcactgca cgcaaccacg gcgcgctgca tccgcatagt tcttgagttc
      121 ccgatcgttc ttgtcgaccg agaggatgta ggcctcgacg gttcggtcga tggcctcttg
      181 tcgttcgaga tggcggccgc agtggccgat gccatgcagc gctgccacct gcacctcgcg
      241 gcagggaacg tcgagcatct cgcgcagcac ttgccagacc gcatctcgcc agcggggctc
      301 gtgccgcacg ttccagaaag tgggccacac gtcgaaccac atgtagcaga cgaaggccag
      361 ccgccccgcc gactcgccga tcggcaaccg cataggcgcc aaccgaggcc cgatgcactg
      421 ccgccacagc gcgggcatgg cctgcatcat gcgcatggcc tcgccgagcg gcacgctcgc
      481 atcgatggcc gcgaacggca cgtcgctgac cgcattgctc atcacgaagt tcaaccccat
      541 gccgacctgc tcgtcgctgt aggcggcgag gtcggtgccg gcctgggcga acagtacggt
      601 ctgtatccgc gtccactcca gcggggtggc ttcgaagtcc ggttcgtcga tgttccagaa
      661 ccactcctgt tcctgcgctt gcggaactgg acggtcgaac aggtagcgca gcgccgccgc
      721 gtacagctcc ggcgcgagtc ccacgccttg cgggctccag tccggagtct gcgcgagacg
      781 gcgccgctgt gcgggcgtag gtctcttggt gttcatggac ttctcgattg tgggttgtct
      841 tggtggacgc gacgggattc gaacccgcat caatgcagca accacggttc ttgagtccag
      901 tggcctcgtc ggcgattgga aacgcgcgcc agtgacagac ccgtcatcgg agccagcacc
      961 tgcatcctca accgtgttcg ccgaccaact ttcctgctgc acctggtgct acccaggctg
     1021 ccggcatgtc aatcatttgt ggcagtggcg ctcccttctg tgccagcgcg cccgttgttc
     1081 aggagtgccc cgccgggcag gcccggcggg gcgggcgctc aacgcagctt caccagcccg
     1141 ttgagcacct ggtccagacc tccgaaaacg gagatcctgt cgatgcgctc ggccacgcgt
     1201 tccagcgtct cgagctcctt cagccgcagg gcgacggggt tgtcctccat caccttggcc
     1261 gtgttcagca gcgagcgcgt cgctgccgtc tcttcacgcc gacggatcac gttggcctcg
     1321 gccgacttgc cggcctcgac cacctgggcg aggatggtct tcatctcgcc cggcagcacg
     1381 atgtccttca cgcccacgcc gtcgagctgc agaccatagt ccgcgagctt cgccttgacc
     1441 tgggcggtca cgacctcgtc gatcaccgtc ttgttctcga gcagctcgtc gagcgagcgg
     1501 gtgcccaccg cggcgcgcag gccgaactgc agctcgcggt acaggtggtc cgccggcttg
     1561 gcgagctcct tgtaggcctt gagcacatcg gtgaagcgcc acgtggccga gaggttcagc
     1621 cgcagggcga ccttgtcgcg ggtcaggatc tcctggcccg tgacctcgag agcctgcagc
     1681 cgcaggtcca cgagttccac cgacacgttg cggttgaact tccagaacgc gtacgagccg
     1741 gccgcgagca gccgctctac cttgccgtcg atccacagca tgccggcgcc gtgttcgggc
     1801 acctgcacct gcagcacgcc ggccaggccc gccacggcgc gttggcgaag ttgcgtctgc
     1861 acgagccgcg ccacgagcgc gggcggcacc tcggccgact cgccgatgtc gatcacctcg
     1921 acctgcacgt cgaccaggcc cttccagtac aggcgccggg tggccggcgc gagcacctcg
     1981 accagcgcgc cgttctcggt ccgcagaccg acctggtgtt cggtgagctc gactcggacg
     2041 aactcggccg cgaccacctc gggctccttg gccatcaggt agtcggccag gccgtgcgtg
     2101 aacgccggct gctcgagcgc gaagagttcc acgcgcaggg tgtcgaggcc ggcgaacagc
     2161 cagtgcgtgc cgggttgcag cacgcgctcg aaatcgccat tgcgcagcag caagccgcgc
     2221 tcgttcttct tcacggtgac acgcttgatc ttcatgggag ttctccttct tggttgaagg
     2281 gtgtgaatga aaagggtgcc gttacagcaa cggccgcggt cgacgagtgc ctgggcccgg
     2341 atcgcacggt gcgcgtcggc cgcccccctc cctgaaggca acctcggctg gccgtggatg
     2401 cgaagccccg cgtgcttggc gcacgagcgg ccgccgcaga tgcacgtttg ggcttgcgcc
     2461 ttgagtgcac gttcggcggt ggtctttcga ccgttcttgg agttggagcg ggagtcgaac
     2521 ccgcgaccga cgcgttatga gcgcgctgct ctacccactg agctagccaa caatggcggt
     2581 gaagccggga gtcgaaccca gacccttgcg ggccagccga tgcatcacca ttccgttgct
     2641 tccagcaagg cggcatgtgc ggcaccgacg gacgtatcca ggtgctgcac accggcgggg
     2701 ttcacgaacc ccaggcctgt gagcgtttgc cttccacaac agactctttg gcgtccgagc
     2761 ctttcagctc aggcgcgatc ccttcggcag gcgcgcagcc agccattcgt gcacgtcggc
     2821 acggatgtgc cggccttcga gcagaaagtc ctcgaagcgg ctcggcacgt acggcaggta
     2881 cacgagcggc atgcccgctt cctccggcgt acggtcggcc ttgcggccgt tgcacgcacc
     2941 gcaggcggtg accaggttca tccaggccca gcggccgccg cgcgattccg gcaggatgtg
     3001 ctcgcactgc aggtcgcgct cgtgaaagcg ctggccgcag taggcgcacg tcatgcggtc
     3061 gcggcggaac agcttgagct tgctgaacgc gggcaccacg tcgaacaggt tgaccctgga
     3121 ggcgccgcgc agcgccatga tcgggaacac gtcgatgacc gactgccgtc cgcgcacgac
     3181 gttgaagccg ccgcgcagcg tggccagcgg cccgcagcca tcgacccacg ccaccgagcc
     3241 cgtggccacg tgaacggccg catgctcgag cgagatccac gcctgcggcg tgccctggat
     3301 gtcgagttgc agcacgtggc tgtggtgttg gtggtgcatg ggtggcctcc cttcatggcc
     3361 gtggttcgga tgaatcgaat gggtgcgccc gggtggactt gaaccaccgg cctcgacctt
     3421 cggagagtcg cgctcttgcc gctgagctac gggcgtttgg cgttggtagc ccctgacgat
     3481 ttcgaaacgt cgaccttcgc tttgtaggag cgttgct
//
LOCUS       DAOORV010000283         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_32582_length_3456_cov_11.190238, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000283 DAOORV010000000
VERSION     DAOORV010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32582_length_3456_cov_11.190238"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV56_19210"
     CDS             <1..253
                     /locus_tag="PKV56_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOX69975.1"
                     /translation="VLGKLERAGVVAASGAACTAGANLPSHVLTAMGEDAVHARAGVR
                     LSLGRETRCEDIDRAIDAAVRSLGPLLESTCAAAESVAA"
     gene            290..508
                     /gene="nifT"
                     /locus_tag="PKV56_19215"
     CDS             290..508
                     /gene="nifT"
                     /locus_tag="PKV56_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HOX69976.1"
                     /translation="MKVMIRRSSAGVLSAYVPKKDLEEPIVQQDKPDLWGGTVTLANG
                     WVLSLPEMADGTSLPITVEARRLSGDVD"
     gene            520..786
                     /locus_tag="PKV56_19220"
     CDS             520..786
                     /locus_tag="PKV56_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69977.1"
                     /translation="MESHVVHDALALAEAAGSVREAAQALRTHYSQLRVVVVDAFDMR
                     DETPAAAGPTRTLYYGASDGHCWQVTSDPAQAAGFFLSEARSHG"
     gene            779..1981
                     /locus_tag="PKV56_19225"
     CDS             779..1981
                     /locus_tag="PKV56_19225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOX69978.1"
                     /translation="MDDLAVMDDARIEEPELDLPDDWLLLCDPDLSEVELQLVQAAMG
                     QPNLSSGTMVEHFERNFAKWLDRRHAVAVASGTLGTWLALRALGIGPGDEVIASPYGW
                     HQVAHAVGLTGARVVFADINYWTGCMDPVRAAAQITPQTRAILVGNVNGHPAAWKEFR
                     ALAEERGIRLIEDSTEAIGSRYQGFMVGSFGDLSVFDFSQPAALCCGEGGMVVTDDDS
                     LAMELRYLRSRSVRDRRSVSVGSRVPMQASISELTAALGAGQLARIDDILAKRKRVEA
                     MYHEQMQSFEGIKPPYVATDVDEVHWMIYTVHLGKRFSVSTCEQIIDDMASQYIEAVL
                     YCQPLHQQFHYIQQGCKRGQFPLVERIADRAVALPFHTHLEPDHVKFIVKTMKDSSVN
                     VGAGAAIY"
     gene            1994..2836
                     /locus_tag="PKV56_19230"
     CDS             1994..2836
                     /locus_tag="PKV56_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOX69979.1"
                     /translation="MSHATLDSLPDALIEGLRAGTLAPYLGPLVLGLCPAVPVPATPL
                     DLATFLNAKTSVPGKIRNRLTAVAQFIENFKHRKTLVTAMNEAFAPMPSPSPLHRWLA
                     SLAAPLIVDTWYDDTMRGALAGRADWAEVQGLSQSEHFGTWVGWYTSSGEVSPDPIET
                     TTVLYKPWGAHGPASNYLVSDTDYVEVLTEIDIQTPIPALVQQRRASLGFVFLGCRFN
                     DQLPRAFARQIMKRSQGPHYAVLPDEPTRMEERFLEEQGITRIAMPLAAAVECLTAGS
                     SVAA"
     gene            2901..3206
                     /locus_tag="PKV56_19235"
     CDS             2901..3206
                     /locus_tag="PKV56_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX69980.1"
                     /translation="MSTLTILPSGKTVEAVPGTNLLQAILDAGEKLVSKCGGEAKCGA
                     CHIFLTGGRKGVSKMTPAENAKLDTIIGIGSKSRLACQMTLLGTEPVTVELLGALSG"
     gene            3253..>3456
                     /locus_tag="PKV56_19240"
     CDS             3253..>3456
                     /locus_tag="PKV56_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX69981.1"
                     /translation="MLDVAIIGGGLCGLALAHSLQARGSDWRLYEARERLGGRVLTAK
                     ASDGTPVDLGPTWFWPDSQPAITR"
BASE COUNT          558 a         1199 c         1139 g          560 t
ORIGIN      
        1 ggtgctgggc aagctcgaac gggccggtgt ggtggcggct tccggtgcgg cctgcacagc
       61 gggcgccaac ctgccttcgc acgtgctgac cgccatgggc gaggacgccg tgcacgcccg
      121 ggccggtgtg cggctgtcgc tgggccggga gacccgttgc gaagacatcg atcgtgcgat
      181 cgatgcggcg gttcggagtc tcggtccgct gctcgaatcc acctgcgccg cggccgaatc
      241 cgtcgcggcc tgaagtccca tttccaccaa ccccacggag tacccgacca tgaaagtgat
      301 gatccgccga agttccgccg gggtgctctc cgcctatgtg cccaagaagg acctggagga
      361 gccgatcgtg cagcaggaca agcccgatct ctggggcggc acggtcacgc tggccaacgg
      421 ctgggtgttg tcgctgcccg aaatggcgga cggcaccagc ctgccgatca ccgtggaagc
      481 gcgtcgcctc tcgggcgacg tcgactgagg ggtccggcca tggaatccca cgtggtccat
      541 gacgccctgg cgctcgccga agcggccggg agcgttcgtg aagcggcgca ggccctgcgc
      601 acgcactact cgcagctgcg cgtcgtggtc gtcgatgcct tcgacatgcg agatgaaacg
      661 ccggccgccg ccggtccgac gcgcacgctg tactacggtg ccagcgatgg tcactgctgg
      721 caggtgacca gcgatccggc gcaggcggcc ggcttcttcc tcagcgaggc gcggtcccat
      781 ggatgatctc gccgtgatgg atgacgcccg gatcgaggag ccggaactcg atctgccgga
      841 cgactggctg ctgctttgcg accccgacct cagcgaggtc gagctgcaac tggtgcaggc
      901 cgcgatgggt cagccgaacc tgtcgtccgg caccatggtc gagcatttcg agcgcaactt
      961 tgcgaagtgg ctggatcgcc gacacgcggt cgcggtggcc agcggcacgc tgggcacctg
     1021 gctggccctg cgcgcgctcg ggatcgggcc gggcgacgag gtcatcgcgt cgccctacgg
     1081 ctggcatcag gtggcgcatg cggtgggcct gaccggggcc cgggtcgtgt tcgccgacat
     1141 caactactgg acgggctgca tggacccggt gcgagcggcg gcccagatca ctccgcagac
     1201 acgggccatc ctggtcggca atgtcaacgg ccatcccgcg gcctggaagg aattccgggc
     1261 gctcgccgag gaacgcggca tccggctcat cgaggattcc accgaggcca tcggctcgcg
     1321 ctaccagggc ttcatggtcg gcagcttcgg cgacctgtcg gtgttcgatt tctcgcagcc
     1381 cgcggcgctt tgctgcgggg agggcggcat ggtcgtcacc gacgacgaca gcctggccat
     1441 ggagctgcgc tacctgagat cgcgttcggt gcgtgatcgc cgctcggtct cggtcggttc
     1501 gcgcgtgccg atgcaggcct cgatcagcga gctcaccgca gcgctgggtg cgggccagct
     1561 ggcgcgcatc gacgacatcc tggccaagcg aaagcgggtc gaggcgatgt accacgagca
     1621 gatgcagagc ttcgagggca tcaagccacc ttacgtggcg accgacgtcg acgaagtgca
     1681 ctggatgatc tacacggtcc atctcggcaa gcgcttttcg gtcagcacct gcgaacagat
     1741 catcgacgac atggcctcgc agtacatcga ggccgtcctc tactgccagc ccctgcacca
     1801 gcagttccat tacatccagc agggctgcaa gcgcggccag ttcccgctgg tcgagcgcat
     1861 cgccgaccgc gccgtggccc tgccgttcca cacccacctc gagcccgacc acgtgaagtt
     1921 catcgtgaag acgatgaagg acagctcggt caatgtcggc gccggtgccg cgatctactg
     1981 aacaaggatc tccatgtctc acgccacgct cgactccctc cccgatgcgc tgatcgaggg
     2041 ccttcgcgcc ggcacgctcg cgccctacct gggcccgctc gtgctcgggc tgtgtcccgc
     2101 cgtgccggtg ccggccaccc cgctggatct cgcgaccttc ctcaacgcga agacctcggt
     2161 gcccggaaag atccgcaacc ggctgacggc cgtggcgcag ttcatcgaga acttcaagca
     2221 ccgcaagacg ctggtgacgg cgatgaacga agcctttgcg ccgatgcctt cgccttcgcc
     2281 actgcaccgc tggctggctt ctctggccgc gcccctgatc gtcgacacct ggtacgacga
     2341 cacgatgcgc ggtgcgctcg cagggcgcgc cgactgggcc gaggtgcagg ggctttcgca
     2401 gtccgagcat ttcggaacct gggtcggctg gtacacgtca tccggcgaag tcagcccgga
     2461 cccgatcgag accaccacgg tgctctacaa gccgtggggc gcgcatggcc cggccagcaa
     2521 ctacctggtc tcggacaccg attacgtcga ggtgctgacc gagatcgaca tccagactcc
     2581 gatccccgcg ctcgtccagc agcgccgcgc cagcctcggc ttcgttttcc tgggctgccg
     2641 cttcaacgac cagctgccgc gcgccttcgc gcgccagatc atgaagcgat cccagggccc
     2701 gcactacgcg gtgctgcccg acgagcccac gcgcatggaa gagcgatttc tcgaggagca
     2761 gggcatcacc cgcatcgcga tgccgcttgc tgcggcggtc gaatgcctga ccgccggatc
     2821 gagcgtcgcg gcctgatacc ggcagctttc gcgaccctgc attcatctca cgtcccacct
     2881 gtcgaaccac ggagtcaccc atgtcaacgc tcaccatcct tccttccggc aagaccgtcg
     2941 aagccgtccc cggcaccaac ctcctgcagg ccatcctcga tgccggcgag aagctggtca
     3001 gcaaatgcgg cggcgaggcc aagtgcggtg cctgccacat cttcctgacc ggcggacgca
     3061 agggcgtctc gaagatgacg ccggcggaga acgccaagct cgacaccatc atcggcatcg
     3121 gcagcaagtc gcgcctcgct tgccagatga cgctgctcgg gaccgaaccc gtcaccgtcg
     3181 aactgctcgg cgccctgtcg ggctgagttg ccgccactgc cgctcgtcgt tgcccgccct
     3241 caggaggttg acatgctcga tgtcgcgatc atcggtggcg ggctgtgcgg cctcgcactg
     3301 gcgcacagcc tgcaggccag gggaagcgac tggcgtctgt acgaagcgcg cgagcggctg
     3361 ggcggccggg tgctgaccgc gaaggcgagc gatggcacgc cggtcgacct gggcccgacc
     3421 tggttctggc ccgacagcca gccggcgatc acgcgc
//
LOCUS       DAOORV010000284         3438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_32813_length_3438_cov_7.149571, whole genome shotgun sequence.
ACCESSION   DAOORV010000284 DAOORV010000000
VERSION     DAOORV010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32813_length_3438_cov_7.149571"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            783..1532
                     /locus_tag="PKV56_19245"
     CDS             783..1532
                     /locus_tag="PKV56_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX69982.1"
                     /translation="MKVLVAVKRVVDYNVKVRVRSDGMGVDIANVKMSMNPFDEIAVE
                     EAVRLKEQGVATEVIAVSCGDAKCQETLRTAMAIGADRGILVETTEELQPLAVAKLLK
                     ALVDKEQPQLVILGKQAIDDDANQTGQMLAALADLPQATFASKVDVAENKVNVTREVD
                     GGLETLSLSLPAVITSDLRLNEPRYVTLPNIMKAKKKQLDTVSPADLGVDVTPRLKTL
                     KVAEPPGRAAGIKVPDVATLVDKLKNEAKVI"
     gene            1544..2482
                     /locus_tag="PKV56_19250"
     CDS             1544..2482
                     /locus_tag="PKV56_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX69983.1"
                     /translation="MTVLVIAEHDHATLKLATLNTVTAAVACQSGDVHVLVAGANAAE
                     AGKAAAQVAGVAKVIVADSPSLAENLAENVAAQVLSIAKNYSHILFSATANGKNVAPR
                     VAAKLDVAQISDITKVDSADTFERPIYAGNAIATVQSSDPVKVITVRTTGFDPAAATG
                     GSAAVESVEGVQDSGKSNFVGREVTKSERPELTAAKIVVSGGRALGGAEKFQEVLAPL
                     ADKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKV
                     IVAINKDEEAPIFSVADYGLVADLFTAVPELQKALG"
     gene            complement(2718..>3438)
                     /locus_tag="PKV56_19255"
     CDS             complement(2718..>3438)
                     /locus_tag="PKV56_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOX69984.1"
                     /translation="RSFSADAPEQTLSEVAARSGLTRAGARRFLLTLQTLAYVEGDGK
                     HYRLTPRILDLGFAYLTSMPIWNLAEPVMERLSDALGESCSAAVLDGSDIVYVMRVHT
                     HKIVSLNLNVGTRLPAYCTSLGRVLLAGLDDAEVKNRLGSSSLSAKTRHTVTDPAEVL
                     ARVQQVRRQGWALVNQELEEGLVSAAAPVKNRAGKVVAALNASWLANRTSATAMKEQL
                     LPKLRTAADDVSALLSRTIRT"
BASE COUNT          684 a         1020 c         1107 g          627 t
ORIGIN      
        1 ctcagcttcg tcctggttgc tgacggtctt cgtggtttgt gttaacacgc cctagtgcag
       61 ggcactggat tcagcaagac cgccccgacg atgtcaaccg tgccgtgacg gcgtggattg
      121 acgccctgta gaggtgaacg ggaccctgat caatgcaaac atcttcgctg cacgagatca
      181 gcgtgcgtag aacgactggg gcacgtccgc catcacgcgc acagtgaaca tgcccagtac
      241 gtcctcggag agcaaacaga tcaattcaca tgcacccctt acttccacag gtttccgagt
      301 gcgactccca agagcaatgt cgttgcgaga cgacggcttc ttcgggcgct caacgcaatc
      361 ccccggcacc gtcacaaggc agctcattgt ggaggcgagt cgctaactac ctgagcgcta
      421 tccgtctcgc gacgaagaag cgttggacag gtggtaagtg actgcggagg gggctgggtt
      481 catctgaggc aatcgcatgc tcgatctatc gactgggtcg gttcgttggt ggagttgatt
      541 ggcctggttg gatcaatttg ctcggagatc cagaggaacc ggttctggtg gcttggcggg
      601 aacgtgcagc ttgctccccg tctgaatcca gccatccggg ggagatgatt tttcttgcgc
      661 gcggagggcg acacctatca gcagccttcg cgcagccgtt cgatgctgat cgcggaccgc
      721 cttcgttttc cgaacgacgt tgggccgcgc gctatttttt tgtatgaagg atcggaaatc
      781 tgatgaaggt gctagtagct gtgaagcgtg tggtggacta caacgtgaag gtccgagttc
      841 gatcggacgg gatgggggtc gacatcgcga acgtgaagat gagcatgaac cctttcgacg
      901 agattgccgt cgaagaggcg gttcggctga aggaacaagg cgtggcgacc gaagtcatcg
      961 ccgtttcctg tggcgatgcg aagtgccagg agacgctgcg caccgcgatg gccattggtg
     1021 cggatcgagg catcctcgtg gaaaccaccg aagaacttca gcctctggcg gtggccaaac
     1081 tcctgaaggc gctggtcgac aaggagcagc ctcagctcgt gatcctcggc aagcaggcga
     1141 tcgatgacga tgcgaaccag accggccaga tgctggcggc tctggctgat ctgccgcaag
     1201 ccaccttcgc atcgaaggtc gacgtggccg agaacaaggt caatgtcacc cgcgaagtcg
     1261 atggcggcct ggaaaccctc tccctctcgc ttcccgccgt catcaccagc gacctgcgcc
     1321 tgaacgagcc gcgctacgtg acgctgccca acatcatgaa agccaagaag aagcagctcg
     1381 atacggtaag tccggcggat ctgggtgtcg atgtcacgcc gcgcctgaag accctgaagg
     1441 ttgctgagcc ccctgggcga gccgcgggca tcaaggtgcc ggatgtggcc acgctggtgg
     1501 acaagctgaa gaacgaagcc aaggtaattt gaggaagact gtcatgaccg tacttgtaat
     1561 tgccgaacac gaccacgcca cgctcaagct ggcaaccctc aataccgtca cggctgcagt
     1621 ggcttgccaa agcggtgacg tccatgtgct ggtggctggt gccaatgccg ccgaagccgg
     1681 caaggctgca gcccaggtgg caggggttgc caaggtaatc gtggcggaca gtccgagcct
     1741 cgctgaaaac ctcgccgaga acgtcgccgc ccaggtgctt tcgatcgcca agaactacag
     1801 ccacatcctg ttctcggcca ccgcaaacgg caagaatgtg gctccgcgcg ttgccgccaa
     1861 gctggatgtg gcccagatca gcgacatcac caaggtggac agcgctgaca cgttcgagcg
     1921 tcccatctat gctggaaacg ccatcgcaac ggttcagagc agcgacccgg tcaaggtgat
     1981 cacggtgcgc acgactggct tcgatcccgc agcagccacg ggcggcagtg ccgcggtgga
     2041 aagcgtggaa ggtgtacagg acagcggcaa atcgaacttc gtgggccggg aggtgaccaa
     2101 gagcgaacgc cccgaactga ccgccgccaa gatcgttgtc agcggcggga gagccctcgg
     2161 cggagccgag aagtttcagg aggtgttggc accgctggca gacaagctgg gcgcggctct
     2221 cggagccagc cgtgcagcgg tggatgctgg gtacgccccc aacgactggc aggtcggcca
     2281 gacgggcaag atcgtggcgc ctcagctcta catcgcggtc ggtatctctg gcgcgatcca
     2341 gcacctcgcc ggcatgaagg actcgaaggt catcgtcgcg atcaacaagg acgaggaagc
     2401 cccgatcttt tcagtggctg actacggcct cgtggccgac ctcttcacgg cggtgcctga
     2461 gctacagaag gcactaggct gacgacagtg gctgcgttga atcctatacg ggtgttgagc
     2521 ggctggccgc tcgtcgcgaa gccgctgcag ggccggggcg tcgcgaccgg gggcgctagc
     2581 ggtggcgtgg ttgactcaat cgcccagcca cgattccttc gcgcagcggg cctggtgctg
     2641 cagactccgt cgaaatgtcc tgaggcgctt tcgaagccga gcttgctcgg caggaaagca
     2701 tcgaattcgt caatcggtca agtgcgtatg gtgcgcgata gcagcgccga gacatcatcg
     2761 gccgccgtgc gtagctttgg caagagttgc tctttcatcg ccgtggcgga ggtgcggttt
     2821 gccagccaag aggcattgag cgctgccacc actttccccg cacggttctt caccggtgcc
     2881 gcggccgaga ccaacccctc ttccagttcc tggttcacca gcgcccagcc ctgtcgacga
     2941 acctgctgca cgcgcgccag gacctcggcc ggatcggtga cagtgtggcg cgtctttgca
     3001 ctcagcgagc tggatccgag gcggttcttc acctcagcgt catccaatcc cgcgagcagc
     3061 actcggccga gagacgtgca gtaggcaggc agccgtgttc ccacattcag attgagcgag
     3121 acgatcttgt gcgtgtgcac gcgcatcaca tagacgatgt cgctgccgtc gagtacagct
     3181 gccgagcatg actcacccaa cgcgtctgag agccgctcca tcaccggctc ggccaggttc
     3241 cagatgggca tggacgtcag gtacgcgaat cccaagtcga ggatgcgcgg cgtgaggcgg
     3301 tagtgcttgc cgtcgccttc gacgtaggcg agggtttgca gcgtcagcaa gaagcgccgc
     3361 gccccggcgc gcgtgaggcc gctgcgcgcc gcgacctcag agagagtttg ctcgggcgcg
     3421 tcggcgctga aagaacgg
//
LOCUS       DAOORV010000285         3429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_32930_length_3429_cov_7.879668, whole genome shotgun sequence.
ACCESSION   DAOORV010000285 DAOORV010000000
VERSION     DAOORV010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32930_length_3429_cov_7.879668"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PKV56_19260"
     CDS             complement(<1..931)
                     /locus_tag="PKV56_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOX69985.1"
                     /translation="MHRSPLRRLALACALFVPLAAAADGVSIRNDAADPATTIFPSDR
                     FTVRDWSNITFRRVALPQPDCAVRPSDCADVAVINTLDGFSTQPRITVPFTGDIDPAT
                     VNSSTVYLLNLGDTMTMRGFGHRVGINQVLWDPATKTLAFESDELLQEHSRYVLIVTD
                     GVRDSRGKRIQAARDEDDDDRGARGRGHSEYRRDLRDAMRHGQRGRDKVVAATLFTTQ
                     STTADLAKISRQIKASTPAPVDFIAGNDGTGAVRSVFPTAQVLGIQFNRQTGAAPVFT
                     PGFLPTPALGVVPGAVAQIAYGKFRSPDYQTAQK"
     gene            complement(1090..1767)
                     /locus_tag="PKV56_19265"
     CDS             complement(1090..1767)
                     /locus_tag="PKV56_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX69986.1"
                     /translation="MKALSTAPNLVEQVRDALLDEIASGQLAPGDRIIQEQIAQALGV
                     SRQPVQQALALLRNQGVLHDAPGRGLIVAPLDPGHVQHMYDMRAVIEGLACRRAAETN
                     AEKAAKLGPALIEAGRKAIAGGSVAKMIAADMKFHEFIYGLSGNPLVAPSLATHLTYT
                     QRVMGEVLIRDETPRDIWNQHAEILDAIAHGDGERAESLARAHITQAAGFMVARLRGQ
                     AAERAPE"
     gene            complement(1788..3341)
                     /locus_tag="PKV56_19270"
     CDS             complement(1788..3341)
                     /locus_tag="PKV56_19270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX69987.1"
                     /translation="MNHLLTLADAVATHARLSPHKLGARDSKRELSFAQWHERASRLA
                     NGLLGLGLEKGDRVALLAYNCVEWMEIYAALARAGLVAVPINFRLTAPEIAYIAQHSE
                     ARACIVQDDLLERVEPARTELAIEARRWIHFGGAAPSGWTAYEALIAAAGTREPAVHV
                     RPADPCALMYTSGTTGRPKGAIRSHEGNTLIALATALEMGFGRDDTALLVMPMCHANS
                     LYFSHTFTHLGAACVIDDRKSFDAEALLATLSREKITFTSLVPTHYIMMLGLPEATKA
                     RYDVSAVHKLMVSSAPVRKDTKLAILDHFRNAQLHELYGSTEAGWVTLLRPDEQIDRL
                     GSVGREWSGSGPIRILDPDGNEVPDGEVGELYSRTPYVFDGYWKNPQKTAEAFRGDWC
                     SVGDLARRDEHGYIHLVDRKSNMIISGGENIYPSEVESVLGAHPKVKDVAVVGVPHDK
                     WGEAVQAVVVLHAQAQASEAEIIDWCRDRIAGYKRPQSILFVDDGDMPRTATGKILHR
                     VLRERIAAR"
BASE COUNT          474 a         1141 c         1230 g          584 t
ORIGIN      
        1 acttctgcgc cgtctggtag tcgggcgagc ggaacttgcc gtaggcgatc tgcgccacgg
       61 cgcccggcac cacgccgaga gccggcgtcg gcaggaagcc cggcgtgaag accggcgcgg
      121 cgccggtctg gcggttgaac tggatgccca gcacctgcgc cgtcgggaac accgagcgca
      181 ccgcgcccgt gccatcgttg ccggcgatga agtccaccgg tgccggcgtg gatgccttga
      241 tctgccgcga gatcttcgcc aggtcggcgg tggtgctctg cgtcgtgaag agcgtggcag
      301 cgaccacctt gtcgcggccg cgctggccgt ggcgcatggc gtcgcgcagg tcgcggcggt
      361 actccgagtg cccgcggcca cgcgcgccgc gatcgtcgtc gtcctcgtcg cgcgccgcct
      421 ggatgcgctt gccgcggctg tcgcgcacgc cgtcggtgac gatcagcacg tagcgcgaat
      481 gttcctgcag cagttcgtcg gattcgaagg cgagcgtctt cgtcgccggg tcccacagca
      541 cctggttgat gccgaccctg tggccgaagc cgcgcatcgt catcgtgtcg ccgaggttga
      601 gcaggtacac cgtgctgctg ttgaccgtgg cggggtcgat gtcgccggtg aagggcacgg
      661 tgatgcgcgg ctgcgtcgag aaaccgtcga gcgtgttgat gacggcgacg tcggcacagt
      721 cgctcgggcg cacggcgcag tcgggctgcg gcagcgccac gcggcggaag gtgatgttcg
      781 accagtcgcg caccgtgaag cggtcgctcg ggaagatggt cgtggccggg tccgctgcgt
      841 cgttgcggat gctcacgccg tcggcagcgg ccgcaagcgg aacgaacaat gcgcaggcga
      901 gcgccagccg gcgcaaaggg gaacggtgca tcgatgtctc cttgacgcgc ggcatgttgg
      961 tgttgtggac agaaggcgcc ggcgcggtct cgcagcatgc gccgtccttg atgggcgtca
     1021 atcgcgtttc ccccgaggcg cttcgcctcg tcgtgtcacc agggtgacag gcgattccgc
     1081 tgggtgcggc tactcgggcg cccgctcggc ggcctggccg cgcaaccgcg cgaccatgaa
     1141 tcccgcggcc tgcgtgatgt gcgcacgggc cagcgactcg gcgcgctcgc cgtcgccgtg
     1201 ggcgatcgca tcgaggatct cggcgtgctg gttccagatg tcgcgcggcg tctcgtcgcg
     1261 gatcagcacc tcgcccatca cgcgctgcgt gtaggtcagg tgcgtggcca gcgacggcgc
     1321 caccagcggg ttgcccgaca ggccgtagat gaactcgtgg aacttcatgt cggcggcgat
     1381 catcttcgcc accgaaccgc cggcgatcgc cttgcgcccg gcttcgatca gagccgggcc
     1441 gagcttcgcg gccttctcgg cgttggtctc ggcggcccgc cggcaggcca ggccctcgat
     1501 caccgcgcgc atgtcgtaca tgtgctgcac atgccccggg tccagcggtg cgacgatcag
     1561 gccgcggccc ggtgcgtcgt gcaggacgcc ctggttgcgc agcagcgcca gcgcctgctg
     1621 cacgggctgg cgcgacaccc cgagcgcctg cgcgatctgc tcctggatga tgcggtcgcc
     1681 cggcgccagc tggcccgagg cgatctcgtc gagcagcgcg tcgcggactt gctcgacgag
     1741 gttgggcgca gtggacaggg ccttcatgga gtcaggagtc tagggcttca gcgcgccgcg
     1801 atgcgctcgc gcagcacgcg gtggaggatc ttgccggtgg ccgtgcgcgg catgtcgccg
     1861 tcgtcgacga acaggatcga ctgcggccgc ttgtatccgg cgatgcggtc gcggcaccag
     1921 tcgatgatct ccgcctcgct ggcctgcgcc tgtgcatgca gcacgaccac ggcctgcacc
     1981 gcctcgcccc acttgtcgtg cggcacgccg accactgcga cgtccttcac cttcgggtgg
     2041 gcgccgagca cgctctccac ctccgacggg tagatgttct cgccgccgct gatgatcatg
     2101 ttgctcttgc ggtcgacgag gtggatgtag ccgtgctcgt cgcgccgcgc caagtcgccc
     2161 accgagcacc agtcgccgcg gaaggcctcg gcggtcttct gcgggttctt ccagtagccg
     2221 tcgaacacgt agggtgtgcg cgaatagagc tcgccgacct cgccatcggg cacttcgttg
     2281 ccgtcggggt cgaggatgcg gatcggacca gagccgctcc attcgcggcc taccgagccg
     2341 agccggtcga tctgctcgtc ggggcgcagc agcgtcaccc agcctgcctc ggtggagccg
     2401 tacagctcgt gcagctgcgc gttgcggaag tgatcgagga tggcgagttt ggtgtccttg
     2461 cgcaccggcg ccgacgagac catcagcttg tgcaccgcgc tcacgtcgta gcgtgccttg
     2521 gtggcctcgg gcaggcccag catcatgatg tagtgcgtcg gcaccagcga ggtgaaggtg
     2581 atcttctcgc gggacagcgt ggccagcagt gcctcggcgt cgaagctctt gcggtcgtcg
     2641 atcacgcagg cagcacccag gtgcgtgaag gtgtggctga agtacagcga gttggcgtgg
     2701 cacatcggca tcaccagcag cgcggtgtcg tcgcggccga agcccatctc cagcgcagtg
     2761 gccagcgcga tcagcgtgtt gccttcgtgg ctgcggattg cgcccttggg tcggccggtg
     2821 gtgcccgagg tgtacatgag cgcgcagggg tcggcaggcc gtacgtgcac cgccggctcc
     2881 cgcgtgccgg cggcggcgat cagcgcctcg taagcggtcc agccggacgg tgccgctccg
     2941 ccgaagtgga tccagcggcg cgcctcgatc gccagctcgg tgcgagccgg ttcgacgcgt
     3001 tcgagcagat cgtcctgcac gatgcaagcg cgcgcctcgc tgtgctgcgc gatgtaggcg
     3061 atctccggcg cggtgaggcg gaagttgatc ggcacggcca ccaacccggc gcgcgccagc
     3121 gccgcgtaga tctccatcca ctcgacgcag ttgtaggcca gcaacgcgac gcggtcgccc
     3181 ttctccagcc cgaggccgag caggccgttg gccagccggc tggctcgttc gtgccattgg
     3241 gcgaagctca gctcgcgttt cgagtcgcgc gcaccgagct tgtgtgggct caggcgggca
     3301 tgcgtggcca cggcatcggc cagggtcagc aggtggttca agcggcgctc cgtggaagga
     3361 catcgggtcc actgatctta gaactctgaa ttcagagttc gatcagggta ttccccgaaa
     3421 ctcgcactc
//
LOCUS       DAOORV010000286         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_33282_length_3403_cov_6.258662, whole genome shotgun sequence.
ACCESSION   DAOORV010000286 DAOORV010000000
VERSION     DAOORV010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33282_length_3403_cov_6.258662"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKV56_19275"
     CDS             <1..606
                     /locus_tag="PKV56_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69988.1"
                     /translation="LHDPFLMKDMDKAVTRIAEAITNHEKILVYGDYDVDGTSAVALV
                     RPSVHLMARCRNPALSRWQGWQLPELQRTAAASAEPPSSSTSPLLRAFCFLAHRQDPI
                     KDGEGKFMAPSSPRRSNRAVVAAGTARSGSAPGPPDRDVIIAIKNSEVLKRCPHSRSI
                     FDDDHISTCKPGCCPSADHAFSGCRDTHTQVHHQWISHLST"
     gene            773..877
                     /locus_tag="PKV56_19280"
     CDS             773..877
                     /locus_tag="PKV56_19280"
                     /inference="COORDINATES: protein motif:HMM:NF017691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HOX69989.1"
                     /translation="MSSCAEARYFLANCPGVKMDGNHDGVPCEQQWCN"
     gene            complement(1031..1966)
                     /locus_tag="PKV56_19285"
     CDS             complement(1031..1966)
                     /locus_tag="PKV56_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOX69990.1"
                     /translation="MRCATLAPSSHNTQCWKFAIDDKGRSITILPDLARRCPAVDPDD
                     HHVFVSLGCAAENLIQAALAHGLKGVALFDPAGDAVRVTLEPTRAQATALYTAIPLRQ
                     CTRGDYDGKPLSSEELALLQRAGSSESVRLLFLTEPQAMEQTLDYVIQGNTAQLADAA
                     FVNELKAWIRFNGAAAVRMRDGLYSVSSGNPNIPSWMGDLAFRWLLTAKGENDKYARQ
                     VRSSAGIAVFVGQAADKAHWVEVGRCYERFALQATALGIRNAFLNQPVEVAALRPPFA
                     AAMGLGGQRPDLVVRFGRGPVLPRSLRRPVEAVLV"
     gene            complement(2307..2672)
                     /locus_tag="PKV56_19290"
     CDS             complement(2307..2672)
                     /locus_tag="PKV56_19290"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOX69991.1"
                     /translation="MTRQPEMPRMNKLTASLLNAALLASMMLAGGCSKPPESAAPTPA
                     TTSSVGNVSDIDVTEHVKTALRQSELLKAFDINVVTLKGDVRLIGMLDNQAQVDEAIK
                     IARAADGAHTIHDELTLKR"
BASE COUNT          633 a         1099 c         1061 g          610 t
ORIGIN      
        1 cttcatgatc ctttcctgat gaaggatatg gataaggccg ttacccgtat cgcggaggcc
       61 atcacgaatc atgaaaaaat actggtttac ggcgattatg atgttgacgg aacatcggca
      121 gtggcgcttg ttcgaccgtc agtccacctg atggcacggt gccggaaccc ggcactttcg
      181 cgctggcagg gttggcaatt gccggaattg cagcgtaccg ccgccgcaag cgctgaaccg
      241 ccatcatcca gcacaagccc gcttctgcgg gctttctgct ttctggcaca ccgccaggac
      301 ccgatcaagg atggcgaagg caagttcatg gccccgagca gtccgaggcg gagcaaccgt
      361 gcagtcgttg cagcaggcac tgctcggtcg ggatcggctc ccggtccgcc agatcgtgat
      421 gtcatcatcg ccatcaagaa ttccgaggtc ctgaagcgtt gcccgcactc gagatccatc
      481 ttcgatgacg accacatatc gacttgcaag cctggctgtt gcccttctgc tgaccacgct
      541 ttcagcggct gccgcgacac ccatacacaa gtgcaccatc aatggatcag tcacctttca
      601 acgtgacccc tgtccctccg ccgaacccag gaagcacccg acgctggagc agttgaacgc
      661 cgaacgaccg aagcgtccgc gtcaaggcgc agccgaatcg agtgccctgc ctgcagccac
      721 tgtcgaaacg aaagccggat tcgcctgcga cggtcgcatt cactgctcac agatgagttc
      781 gtgtgccgag gccaggtact tcctggcgaa ctgcccgggg gtcaagatgg acggcaatca
      841 cgacggggtt ccctgcgaac agcagtggtg caactgatct gcgtcagctc ccagcgaccg
      901 atcgagcgca cgcaagagtc tccaggaagc cgacgtctct acaggagcac gcacaaccga
      961 tgccccgggg cgctccccag cctccgcagc gccttcattg caattgcctt ggctcggggc
     1021 ggtctgtcga tcacaccaga acggcctcca ccggtcgcct aagcgagcga ggcagcaccg
     1081 ggccgcgacc gaagcgcacg acgaggtccg gtcgctgccc acccaggccc attgcggcag
     1141 caaagggcgg acgcagcgcc gccacctcga cgggctggtt caagaaggcg ttgcgaatgc
     1201 ccagcgcggt ggcctgcagg gcgaagcgct cgtagcagcg ccccacctcc acccagtgcg
     1261 ccttgtcggc ggcctggccg acaaaaaccg cgatgccggc ggaactgcgc acctgccgcg
     1321 cgtacttgtc gttctcaccc ttcgcggtga ggagccagcg aaacgccaga tcacccatcc
     1381 aggacgggat gttcgggttg cccgaggaca cgctgtacag cccgtcgcgc atgcgcacgg
     1441 cagcggcgcc gttgaaacgg atccaggcct tcagttcgtt gacgaaggca gcgtcggcca
     1501 gttgcgccgt gttgccttga atcacgtagt ccagcgtctg ttccatcgcc tggggctccg
     1561 tcagaaacag caggcgaacc gattccgaac tgccggcgcg ctgcagcagc gccagttctt
     1621 cgctggacaa gggcttgccg tcgtagtcac cgcgggtgca ctggcgaagg ggaatggccg
     1681 tgtacaaggc ggtggcctgc gctcgcgtgg gctcgagcgt cacgcgcacg gcatcgccgg
     1741 ccgggtcgaa cagcgcgacg cccttgagtc cgtgggcgag cgcggcctgg atcaggttct
     1801 cggcggcaca gcccagcgag acaaagacgt gatggtcgtc cgggtccacc gcagggcacc
     1861 gccgcgcgag atcgggcagg atggtgatcg accggccctt gtcgtcaatc gcgaacttcc
     1921 agcactgtgt gttgtggctg gacggagcca gggtggcgca gcgcaccagc tcgcgaccaa
     1981 gcgcgacccc atcgagattc gtcgccgcgc ccaactgcca ggtgcgctcg gcagccgctt
     2041 cgtagctgtc cgttgccagc ttcgggggag cagcctgaca gcgccagcgg gccggccaac
     2101 agccacggcg tcgaagcggc gaactgcctg cgtgtgatca tgatctcgat gtcaggtgcg
     2161 gttggccggt ggtggcaatc tgagcgacaa gcgcctccgc tgctcagcgt aatcgatcag
     2221 ggcccctgcg tgttgtggct cgcctgggga tcaataactc gactgatcga agtcgaccca
     2281 tcacgaggcc tgagcatggc cgcgcgctat ctcttgagcg tgagttcatc gtgaatggtg
     2341 tgggcaccgt cagcggcgcg cgcgatcttg attgcctcat cgacttgagc ctggttgtcg
     2401 agcattccga tcagccggac gtcgcccttc aacgtcacga cgttgatgtc gaaggccttg
     2461 agcaattcgc tttgccgcag ggcagtcttc acgtgctcgg tcacgtcgat gtccgagaca
     2521 ttgccgacgg atgacgtggt ggcaggcgtc ggtgccgcag actccggcgg cttgctgcag
     2581 ccccccgcca acatcattga ggcgagcaag gcggcgttga gtaaagaggc ggtgagtttg
     2641 ttcattcgag gcatctccgg ctgtctggtc accttggcgt gacctagctt ctaccgtcgc
     2701 atgttcgagc ccgcgcgtcc gtgcgctgcc gaacatcgtc gccaactcct cggttcacga
     2761 accccgttca ttcgaaagtc acgaagttgc ccctgccccc ggcacgccgc aggtctgcac
     2821 atcgaggtgc gggtcgcagc gatctggtcg gcggcagtgc tgacgagcga taagggtgac
     2881 gagatcgcgt tgatcgcggt gccggtgcag ttgaagcgct gcgggacggc ggcgcgactg
     2941 attccaacga acagggaatc aacagggaca ggtcgacatg ccgctcagcg ccccccggat
     3001 ttcactccgc aagccccggg tgctggtgat cgagctggat caacccactt gatcggaggg
     3061 aaccgaccca cgacgaaacc aggcaacttc aggcgcttct tgactctcca gcctgccaac
     3121 ctggcccact ggaacgtgac catgtgggtc aaatcgacgc gatcgtcaac acacgtgggg
     3181 cagatcgctg cgttctgctg cacgaatgac cttcgttaca gtgacagttg tccatcgccc
     3241 tgaacgccat catgagtcga ctgcaaatcg aatttcgtcg cctcttcgtt gccaccgagg
     3301 cggcctccac tgacgcggcc atcaccgcgt cggacctggc cgattcagaa ggtcgcgtgc
     3361 gtgccctggt gctggagctg gctcgtccgg ccgactggca gca
//
LOCUS       DAOORV010000287         3396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_33368_length_3396_cov_8.962287, whole genome shotgun sequence.
ACCESSION   DAOORV010000287 DAOORV010000000
VERSION     DAOORV010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33368_length_3396_cov_8.962287"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..1185)
                     /locus_tag="PKV56_19295"
     CDS             complement(424..1185)
                     /locus_tag="PKV56_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69992.1"
                     /translation="MLPVIAQHLTRPGAAAVEVAADHWLVVVSQTCDVVARTLEAEPF
                     VEILHCKPIKKPRAQYRDFRSTRILDFRPNRETHEAVALSAHAVADRYLVPRELFRDQ
                     PPDPDRHLSGEAAQRIMAWYALRAGRPSWPDAFVKRIDKDTEDALEAAIEPLKEDIAE
                     VRLGIVEKDDELGDDQSYHVAVYFVVDAEIWEGDVEGRAAINAAFATFVSKLNECDGI
                     EVDQNLSEVVSGDEFTWQETRATDEWNFANLSHRE"
     gene            complement(1256..2023)
                     /locus_tag="PKV56_19300"
     CDS             complement(1256..2023)
                     /locus_tag="PKV56_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX69993.1"
                     /translation="MSDTIEARFYNAQRATLLQRRPSTIIDAAESSHPAVPMQFYGGG
                     ETHLIGSYRMGTKLTGARTVLIVDTDVLLATSAIPTVSVEDFDADSWSRWSKASKEAF
                     QKATAAAKAAPSAAARLRVERLAALQAALGLSTSNLAQALGLSRPGLYKWLDASSDVK
                     LQGASRERLAAVERIAKQWRERTTAPLSSVSNEPLADGRTVLSMMVADQFDEATVVGA
                     FDELLAKLAGKPKTRSQKLAEAGFKRRPSAKSLPSDE"
     gene            complement(2020..2802)
                     /locus_tag="PKV56_19305"
     CDS             complement(2020..2802)
                     /locus_tag="PKV56_19305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HOX69994.1"
                     /translation="MPTTTLGTWRKPPLVYVVAELIISPYYSMATKVPGLQDRLRSTF
                     PKTIEGQELVVDAAKPSAQPIWQLISADQKHGVQLSTRSISLHATSYVNSADLMTRWA
                     DVLDAIHEAGLGAFVERAGLRYVDLILPSGDRSPAEYLAQQLQGIAPDGAKPTGSLWA
                     AAFLFDGATVNLRAGAPAPAGMLLPPNFNAMPLHKPQVMVDCEKRVRDEKAIGFIDTD
                     CLRDINQVFDAAQLVGIYTDMQKLTSRTFKAALSATANKEWM"
BASE COUNT          613 a         1070 c         1083 g          630 t
ORIGIN      
        1 ccgctggacc cggagcacaa gcgcatgttc atggccatga aacgcgaggt gctgcgcctt
       61 ggtgggtacg cgtgcgagtt cttcgtgagc cagacccccg agctcaccgc gatggccgat
      121 cgcatcatcg acctggaaag ctattctgcg cagggtgcgc aggtggtcgc atgactgaag
      181 gcaatccaag ggccgccccg tcgtacccct tggaccaccg agcggcgacg cgtgccagtg
      241 aacgtcgcgt agtcgcagca acgctgcctt ctgttcggtc gcatcggtcg gtcggcgctg
      301 gacggccagc aggcgccgtt cacgcctgac aggttaccgg cgagctatcg gtgtcgtatg
      361 caagaggatg ccggctgcgt agcgataccg cgagagagtc aggcaaaacc aactgcggat
      421 cactcactcg cgatggctca ggtttgcgaa gttccactca tcggtagccc tcgtctcctg
      481 ccaggtgaat tcatcgcccg acacgacctc actgagattc tgatccacct cgatcccgtc
      541 gcattcgttg agcttgctga cgaacgttgc gaaggccgca ttgatcgccg ctctgccctc
      601 aacgtcgccc tcccagatct ccgcgtcaac gacgaaatac acggccacgt ggtacgactg
      661 atcgtctccc agttcatcgt ccttctcaac aatgccaagt cggacctcgg caatgtcttc
      721 cttgagcggc tcgatcgcgg cctcgagcgc gtcctcggtg tccttgtcga tccgcttcac
      781 gaaggcgtcc ggccaactgg ggcgacccgc gcgcagcgcg taccacgcca tgatgcgttg
      841 cgctgcctcg ccgctcaaat ggcgatcggg gtccggtggc tggtccctaa aaagttcacg
      901 aggaacaagg tatctgtcgg caaccgcgtg agccgagagc gcaacggcct cgtgagtttc
      961 gcggttcggc cggaagtcaa ggatgcgtgt cgaccggaag tcgcggtatt gcgcccgcgg
     1021 cttcttgatc ggcttgcaat gcaagatctc aacgaacggc tcagcctcga gagtgcgagc
     1081 cactacgtcg caggtctgcg agacgacgac cagccagtga tccgctgcca cttcgacggc
     1141 ggccgcgcca ggccgagtaa ggtgctgcgc gatgaccggc agcatcccct gaggcactac
     1201 ggccccggtg caccagccct gctctgcaac ctcgtctcca aacggcaagg cgtcctcact
     1261 cgtcggacgg cagcgacttg gcggatggac ggcgcttgaa gccggcttcg gcgagcttct
     1321 ggctgcgagt cttcggcttg ccggccagct tggccaagag ctcgtcgaac gcgccaacaa
     1381 cggtggcctc gtcgaactgg tcggcaacca tcatcgaaag gacggtgcgt ccgtccgcga
     1441 gaggctcatt ggagacggag ctcagcggag ccgtcgtccg ttcgcgccac tgcttcgcaa
     1501 tgcgctcgac cgcagccagg cgctcacgac tggcgccctg cagcttcacg tcgctggagg
     1561 catcgagcca cttgtagagg ccgggccgag acagaccaag cgcctgggcc aggttcgacg
     1621 tcgacagtcc cagagcggcc tgaagagcgg ccagccgttc tacgcgaagt cgagcagctg
     1681 cgcttggcgc tgccttggct gcagcggtcg ccttctgaaa ggcctccttc gaggcctttg
     1741 accaacgcga ccacgagtca gcgtcgaagt cttcaactga gacggtcgga atcgccgatg
     1801 tcgcgagcaa cacgtccgtg tcgacgatca ggacagtccg cgcgccggtc aacttggtcc
     1861 ccatgcgata ggacccaatg aggtgcgtct caccgcctcc atagaactgc atcggcacag
     1921 ctggatgaga cgactcggcg gcatcgatga ttgtgctcgg acgacgctga agcagcgtcg
     1981 cacgctgagc gttgtagaac cgtgcctcga tcgtgtcgct cacatccact ccttgtttgc
     2041 cgtggcagac agcgcagcct tgaaggtccg cgaagtcagc ttctgcatat cagtgtagat
     2101 gccgaccaat tgggcggcgt cgaagacttg gttgatgtcg cgcaggcagt ccgtgtcgat
     2161 gaagccaatc gccttctcgt ccctgaccct cttctcgcag tcaaccatca cctgcggctt
     2221 gtgcagcggc atcgcgttga agttcggagg caacagcatc ccagccggcg ccggcgcgcc
     2281 cgctcggagg ttgacggtgg cgccgtcgaa cagaaatgcg gccgcccaca gcgacccggt
     2341 cggcttcgcg ccgtctggag cgatgccctg cagctgctgg gccaggtatt ccgccggcga
     2401 gcgatcgccg ctcgggagga tgaggtcgac gtagcgcaac ccagcgcgtt ccacgaacgc
     2461 accgagacct gcctcgtgga tggcgtcgag gacgtcggcc catcgggtca tcaggtccgc
     2521 ggagttgacg tagctcgttg cgtgcagtga gatcgacctg gtactgagct ggacgccgtg
     2581 cttctggtcg gctgagatca gctgccagat cggctgggcg gaaggcttgg ccgcgtcgac
     2641 gacaagttcc tgcccttcga tggtcttcgg gaaggtggaa cgtagtcggt cttgcagacc
     2701 aggcaccttc gtcgccatcg agtagtacgg cgaaatgatc agctctgcga ccacatagac
     2761 gagcgggggc ttacgccacg taccgagcgt ggtggtgggc atgcgggctc tccttcggtg
     2821 cgttgcagtt gcctgtagtg ctctcttcga agcacgtgga aattagttta cagggtagtt
     2881 gacacccgtg gcgtgaaatc gttccgggtt tacacgcgct tcaccggcac gtccagcaag
     2941 acacctgtct tagacgtcgc taagtctttg attttgaagg tgcaacatgg cttcgacggg
     3001 tgcggcctga ctcgggcggc cccatctcgg gccgcgagcc gcgggctgcg tgtggcggcg
     3061 gcggtggcgg tggcgtcgag cttcatgacg acggaccaag catcttgatg ggccgtagcc
     3121 gtccgagcgc aaggcacctg cgttcacgcc aggcgcgcat agcgacttgg ttccagaggt
     3181 ggctgtaggc ccaaacaaca gcctcccttg agcctccgtg gacacggggc tcaaggaaag
     3241 cgcctgaact gtcgctcagg ggatagagaa tcggactcag ctcgccggca gaccgctgac
     3301 ggctgccagt tgccagccgc gcaggtcggg gaacctgtcg gcgaagtcga agatcaggtt
     3361 gcgccgctcg ttggagagcg ccactcgcgc gaacca
//
LOCUS       DAOORV010000288         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_33383_length_3395_cov_5.806287, whole genome shotgun sequence.
ACCESSION   DAOORV010000288 DAOORV010000000
VERSION     DAOORV010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33383_length_3395_cov_5.806287"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKV56_19310"
     CDS             <1..487
                     /locus_tag="PKV56_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOX69995.1"
                     /translation="LLEPLRDLFKDEVRELGVALGLPPEMVYRHPFPGPGLGVRILGE
                     VKKEYADLLRQADAIFIEELHNFKDEATGKTWYQLTSQAFTVFLPVKSVGVMGDGRTY
                     DYVVALRAVQTSDFMTADWAELPYALLKKVSSRIINEVRGINRVAYDVSSKPPATIEW
                     E"
     gene            784..2040
                     /locus_tag="PKV56_19315"
     CDS             784..2040
                     /locus_tag="PKV56_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX69996.1"
                     /translation="MLTETTLKHLKPKDAVYKVADRDGMYVTVSVVGTISFRFDYRLN
                     GRRETLTLGRYGPDGISLAVARERCVDARRAISEGRSPAQEKQREKRRLAEAQTFQEF
                     SDQWAAGARMAASTRSMRMSVLSRDILPAFKNRMLTEITADDLRALCNKVKARGAPAT
                     AVHVRDIVKQIYGFAILHGEKVANPADDVGPSSIATFVPKDRALSPTEIRLMHQQLEF
                     VASYPTIRLALRMILLTLVRKSELIQATWDEVDFANALWTIPKERMKGAKAHSVYLSQ
                     QALDIMIALHTCAGGSAYVLPSRYDADKPMSNATLNRVTGLVVERAKAKGLPLESFTV
                     HDLRRTGSTMLNELGFNSDWIEKCLAHEHGRSSRGVYNKAEYAEQRRHMLQEWADMID
                     AWVAGKPHTPTLLPPSMKVITTQALI"
     gene            complement(2018..2284)
                     /locus_tag="PKV56_19320"
                     /pseudo
     CDS             complement(2018..2284)
                     /locus_tag="PKV56_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
BASE COUNT          667 a          973 c         1126 g          629 t
ORIGIN      
        1 gttgctggag cctctgcgtg atttgttcaa ggacgaagtg cgagaactgg gtgtggcctt
       61 gggtctgccg cccgagatgg tgtaccgcca ccccttcccg gggcctggcc tgggcgtgcg
      121 cattttgggt gaagtcaaaa aagaatacgc cgacctgctg cgccaggccg acgccatctt
      181 catcgaagaa ctgcacaact tcaaagatga ggccacaggc aaaacctggt accagctcac
      241 cagccaggcc ttcacggtgt tcctgccagt gaagagcgtg ggcgtgatgg gcgatggccg
      301 cacctacgac tacgtggtgg ccctgcgcgc agtgcaaacc agcgacttca tgacggccga
      361 ctgggccgag ctgccctatg cgctgctcaa gaaggtctcc agccgcatca tcaatgaggt
      421 ccgcggcatc aaccgtgtgg cctatgacgt gagcagcaag ccgccggcca ccatcgagtg
      481 ggaatgattt ggggcctctc ggtacctttc gccgactatc ggaaaagttt gttaagttat
      541 tgattcatat agaaaattgt ctctaagact atcggtgact atcggcggca agcaggaatt
      601 tttgacgatg aaattgacgg tcatcggaag cgccgccaag ctcgctgaat ttttaccgtc
      661 agacgttttc agggtctgac ggtaaaaaaa gttcaagaaa gctaggatcc atgcgggttt
      721 ccggccgatt ggagcttttc cggtctctga cggtaaaatt ctccgcttcg aggaggatcg
      781 gcgatgctga ccgagaccac gctgaagcac ctcaagccga aggacgccgt gtacaaggtg
      841 gcggaccggg acggcatgta cgtcaccgtg tcggtggtgg ggacgatcag ctttcgcttc
      901 gactacaggc tgaacggacg ccgggagacg ttgacgctgg gtcgctacgg ccctgacggc
      961 atttctctag cggtcgcgcg cgaacggtgc gtggatgccc ggcgcgcgat ctcggagggc
     1021 cggtcaccgg cgcaagagaa gcagcgcgaa aagcgccgcc tcgctgaggc gcagaccttc
     1081 caggagttca gcgatcagtg ggcggctggg gcacgcatgg cagcgagcac gcggtcgatg
     1141 cgcatgagtg tgctgtcgcg cgacatcctt cccgcgttca agaacaggat gctcaccgag
     1201 atcacggcgg acgatctgcg agcactgtgc aacaaggtca aggcgcgcgg tgcgccggcc
     1261 accgcggtcc atgtccgcga catcgtcaag cagatctatg gtttcgcgat cctgcatggc
     1321 gaaaaggtgg cgaacccggc agacgatgtc gggccttctt cgattgcgac cttcgtcccg
     1381 aaggatcgcg cactgtcgcc cacggaaatc cggttgatgc accagcaact cgaattcgtg
     1441 gcgtcttacc ccacgatccg gcttgcgttg cggatgatcc tcctgacgct ggtgcgcaag
     1501 agcgaactga tccaggccac ctgggatgag gtcgacttcg ccaacgcact ctggaccatt
     1561 cccaaggaac ggatgaaggg cgccaaggcc catagcgtgt atctgtcgca gcaggccctg
     1621 gacatcatga tcgcgttgca cacctgcgcc ggagggtcgg cctacgtcct gccttcgcgg
     1681 tacgacgccg acaagccaat gtccaacgcc acgctgaatc gggtcaccgg cctggtggtc
     1741 gagcgagcga aggcgaaggg cctgccgctc gagagcttca ccgtgcacga cttgcggcgc
     1801 acgggctcga cgatgctcaa cgaactgggt ttcaacagcg actggatcga gaagtgccta
     1861 gcgcatgagc acgggcgatc ttcgcgcggg gtctacaaca aggccgagta cgcagaacag
     1921 cgtcgacaca tgctccagga gtgggccgac atgatcgacg catgggtggc gggcaagccc
     1981 catacgccaa ccctgctgcc gccatcgatg aaggtcatca cgacgcaggc gctgatctga
     2041 cgggcctgct gcgtctctgc cgtacgtcgg gcggtggcgc cagtttgatc tgggcgagat
     2101 cggatgcctg gcgtcgagag tcgatccagg cttcgacttc tcggagatcc caaacaacgc
     2161 agcgcgaggt caggtggaac cttcgcggaa actcgccgcg tcgctccatg tcatagatcg
     2221 tggtgtctgc cagcggcacg atctgtcgca gttgggctcg gcgtatggtc ggtcgcaatg
     2281 gtatccagcc ggcatgggca gcggttcgcg ggagtgttgc aggcgcatcc tcaggctgac
     2341 gaacttcgtc agctgaggta tggggatgag aggtcggtgc ggatggtctg tcgcggctgg
     2401 tgctgctcat gatcgactcc ggaaggtggc actggcgtca atttcttccc tggtggtgca
     2461 ctgagctcgc gtcgaaggct gcctgcctgg cagcgggagg tcccgatgcc acggccatct
     2521 tggcggaatc ggtctcgaag aagtcgagca gaaggtgcgg gagacattga cggtggttgg
     2581 gttggctgaa ggttcgggtt ggagggttgt ctcaaatgga aatgaacggt gggcggggcg
     2641 cccgcggtgt ggtgccggtt ggctactgag gcacttgtga tcggtgacgg ccaaaacggc
     2701 gcactggcca ctcgtcccag ggatgcttcg acacgttctc gcgggggcga ccagtccgat
     2761 gtgtggtgca aggggaacag gacgaagtcg gagctgctcg acgagtcatg aaacggatcc
     2821 tcgcgcccgt cggccttggc cgtcgtgcaa gggtggtcac gttccaggga acaccgcgaa
     2881 gcagcgcgct tcctgcgcgc gcggcgcaga ttcaaggacg ctctgtcgca aggacagcgc
     2941 ggcagcggca cgagcccagt gggcggcctg aaaggagtgg tgctcgatgc agagtcagcc
     3001 gatgagacgg cttgaccgac agggtcggtt ctatcggatg gagggtcacg acctctaacg
     3061 gctgctcagg gttcggaaca ggtggcgttg tcagcagggg cctgcagccg aacctcaccg
     3121 tcgttggcag cgaaacgcga cggccgaagc cattggatcg ccgccctcat tcgcagtcaa
     3181 gcaaagcagc tcatctgacc agcttcgacc aagtgatcct gcgacatcga cgatgccggt
     3241 gctgcccgca atggccactt ggagtccacg gaaaaagagc ggccccaccg cagacacgat
     3301 ggggccggaa cggcagcctt aggccgccag cgattcttca gatgcttcca ggccctgggt
     3361 gccggcctcg tccttgtcga ggtccagttc ggcag
//
LOCUS       DAOORV010000289         3271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_35084_length_3271_cov_5.904851, whole genome shotgun sequence.
ACCESSION   DAOORV010000289 DAOORV010000000
VERSION     DAOORV010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35084_length_3271_cov_5.904851"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3154)
                     /locus_tag="PKV56_19325"
     CDS             complement(<1..3154)
                     /locus_tag="PKV56_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOX69997.1"
                     /translation="MSHFLDRLNYFSQPREAFSGDHGVTTGEDRTWEDAYRNRWAHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRWDMPNHEPRGCARGASYSWY
                     LYSANRVKYPMVRGRLLKSWREARLVHDPVDAWASIVENDAKRRDYQQVRGLGGFVRS
                     TWDEVNEIVAAANVHTIKKHGADRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYD
                     WYCDLPPSSPQVWGEQTDVPESADWYNSSFIIAWGSNVPQTRTPDAHFFTEVRYKGAK
                     TVAVTPDYSEVAKLADIWMSPKQGTDAAVAMAMGHVILKEFYFPDRGERSAYFDDYVR
                     RYTDMPMLVMLKEHTLPSGEVVMVPDRYVRASDFNGKLGQANNPEWKTVAFDEGGKVV
                     LPNGAIGFRWGPDGRADEGQWNLEAKEARHGQAVKLKLSLLEGEQPSSATARVGFPYF
                     GGIESANFPNSVLKGDSGDVIVRTVPIQRISLGKAGEERSALVATVFDLQVANYGVAR
                     GLPGEFAAANFDEDTPYTPAWQERITGTPRAQLITVARQFAENAHKTNGKSMVIIGAA
                     MNHWYHSDMNYRGVINMLMMCGCIGQSGGGWAHYVGQEKLRPQTGWTALAFALDWIRP
                     PRQMNSTSFFYAHTDQWRYEKLGMDEVLSPLADKKLYAGSMIDYNVRAERMGWLPSAP
                     QLQTNPMQVVKDAAAAGMEPKDYAVQGLKDGTLKLSCTDPDHPDNWPRNMFVWRSNIL
                     GSSGKGHEYFLKHLLGTSNGVQGKDLGVEEAKPTEVVWHDKAPEGKLDLLVTLDFRMS
                     TTCLYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPAWQSRSDWDIYKGFAKKFS
                     EVCVGHLGVERELVLTPLMHDSPSELAQPFDVKDWKRGECDLIPGKTAPNIQVVERDY
                     PNVFKRFTALGPLMNKVGNGGKGISWNTQTEVKQLGELSGLVTAEGVTCGMPKIETDI
                     DACEVILQLAPETNGHVAVKAWEALGKQTGLDHTHLAIHREDEKIRFRDVQAQPRKII
                     SSPTWSGIESETVSYNAGYTN"
BASE COUNT          497 a         1066 c         1061 g          647 t
ORIGIN      
        1 cgttggtgta gccggcgttg tacgagacgg tctcgctctc gatgccgctc caggtcggcg
       61 agctgatgat cttgcgcggc tgcgcctgca catcgcggaa gcggatcttc tcgtcctcgc
      121 ggtggatcgc caggtgcgtg tgatcgaggc cggtctgctt gccgagcgct tcccaggcct
      181 tgaccgccac atggccgttg gtctctggcg cgagctgcag gatcacctcg cacgcatcga
      241 tgtcggtctc gatcttcggc atgccgcagg tcacgccttc ggcggtgacc agaccactca
      301 actcaccgag ctgcttcacc tcggtctgcg tgttccagct gatgcccttg ccgccgttgc
      361 cgaccttgtt catcagcggg ccgagtgcgg tgaaccgctt gaagacgttc ggatagtcgc
      421 gttcgacgac ctggatgttg ggcgccgtct ttccggggat cagatcgcat tcgccacgct
      481 tccagtcctt gacgtcgaac ggctgcgcca gttcggacgg gctgtcgtgc atcagcgggg
      541 tcagcaccag ttcgcgctcg acaccgaggt gaccgacgca gacctcgctg aacttcttcg
      601 cgaacccctt gtagatgtcc cagtcgctgc gcgactgcca cgccggatcg accgccgtcg
      661 acagcgggtg gatgaacggg tgcatgtcgc tggtgttgag atcgttcttc tcgtaccagg
      721 tggcggtggg cagcacgatg tcgctgtaca ggcacgtggt gctcatgcgg aagtcgagcg
      781 tgaccagcag gtcgagcttg ccttccggcg ccttgtcgtg ccagacgact tcggtgggct
      841 tggcttcttc cacgcccagg tccttgccct gcacgccgtt gctggtgccg agcaggtgct
      901 tcaggaaata ctcgtggccc ttgccggacg atcccaggat gttggagcgc cacacgaaca
      961 tgttgcgcgg ccagttgtcc gggtggtccg gatcggtgca gctcagcttg agcgtgccgt
     1021 ccttgaggcc ctggactgcg tagtccttcg gctccatgcc tgcggcagcg gcatccttca
     1081 ccacctgcat cggattcgtc tgcagctgcg gtgccgaagg cagccagccc atgcgctcgg
     1141 cgcgcacgtt gtagtcgatc atgctgccgg catacagctt cttgtcggcc agcggcgaca
     1201 gcacctcgtc catgccgagc ttctcgtagc gccactggtc ggtgtgcgca tagaagaagc
     1261 tggtgctgtt catctggcgc ggcgggcgga tccagtcgag cgcgaaggcc agcgccgtcc
     1321 agccggtctg cggccgcagt ttctcctgac ccacgtagtg cgcccagccg ccaccgctct
     1381 gcccgatgca gccgcacatc atcagcatgt tgatgacgcc gcggtagttc atgtcgctgt
     1441 gataccagtg gttcatcgcg gcgccgatga tcaccatcga cttgccgttc gtcttgtgcg
     1501 cgttctcggc gaactggcgg gccaccgtga tcagctgcgc gcgtggcgtg ccggtgatgc
     1561 gctcctgcca ggccggcgtg tacggcgtgt cctcgtcgaa attggccgcg gcgaactcac
     1621 ccggcaagcc gcgcgcgacg ccgtagttgg cgacctgcag gtcgaacacc gtggccacga
     1681 gcgcgctgcg ttcctcgccg gccttgccca gcgagatgcg ctggatcggc acggtgcgca
     1741 cgatcacgtc gccgctgtca cccttcaaca cgctgttcgg gaaattggcg ctctcgatgc
     1801 cgccgaagta cgggaagccg acacgcgccg tcgcgctgct cggctgctcg ccctcgagca
     1861 gcgacagctt gagcttcacg gcctgaccgt gacgcgcttc cttcgcctcg aggttccact
     1921 ggccttcgtc ggcccgtccg tcgggacccc agcggaagcc gatcgcgccg ttgggcagca
     1981 ccaccttgcc accttcgtcg aaggcgacgg tcttccattc ggggttgttg gcctggccga
     2041 gcttgccgtt gaagtcggac gcgcgaacat agcggtcggg caccatgacg acctcgccgc
     2101 tgggcagcgt gtgctccttg agcatcacca gcatcggcat gtcggtgtag cggcgcacgt
     2161 agtcgtcgaa ataggcgctg cgctcacccc ggtccgggaa atagaactcc ttcaggatca
     2221 cgtggcccat cgccatcgcg acggctgcat cggtgccctg cttcgggctc atccagatgt
     2281 cggccagctt ggcgacctcg gaatagtcgg gcgtcaccgc gaccgtcttc gcacccttgt
     2341 agcgaacctc ggtgaagaag tgcgcatcgg gcgtgcgcgt ctgcggcacg ttcgagcccc
     2401 aggcgatgat gaaactgctg ttgtaccaat cggccgattc gggcacgtcg gtctgctcgc
     2461 cccagacctg cgggctggac ggcggcaggt cgcagtacca gtcgtagaag ctcatgcaga
     2521 cgccgccgat caggctcagg tagcgcgagc cggcggcgta gctgaccatg ctcatcgccg
     2581 ggatcggcga gaagccgatc acgcgatcgg caccatgctt cttgatcgtg tggacgttgg
     2641 ccgcggcgac gatctcgttg acctcgtccc aggtcgagcg cacgaagccg ccgaggccgc
     2701 gcacctgctg gtagtcgcgg cgcttcgcat cgttctcgac aatgctggcc catgcatcga
     2761 ccggatcgtg caccagacgc gcctcgcgcc atgacttgag cagccggccg cgcaccatcg
     2821 ggtacttgac ccggttcgcg ctgtacaggt accagctgta gctcgcgccg cgcgcgcagc
     2881 cacgcggctc gtggttgggc atgtcccagc gggtgcgcgg atagtcggtc tgctgcgttt
     2941 cccaggtgac gatgccgccc ttcacgtaga tcttccacga acacgaaccg gtgcagttca
     3001 caccgtgggt gctgcgcacg atcttgtcgt gcgcccagcg gttgcggtag gcatcctccc
     3061 aggtccggtc ttcaccggtg gtgacgccgt ggtcgcccga gaaggcctcg cggggctgcg
     3121 agaaatagtt gaggcgatcg aggaaatggc tcatgtgata tctcctggga atttcgtggc
     3181 tggggggcgc atcaagggtt cttcacgtac gcattcgcgc gcaggtagaa ccaccagttc
     3241 agcaccaggc acagggcgta gaagatggcg a
//
LOCUS       DAOORV010000290         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_35109_length_3269_cov_9.185439, whole genome shotgun sequence.
ACCESSION   DAOORV010000290 DAOORV010000000
VERSION     DAOORV010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35109_length_3269_cov_9.185439"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2210)
                     /gene="recC"
                     /locus_tag="PKV56_19330"
     CDS             complement(<1..2210)
                     /gene="recC"
                     /locus_tag="PKV56_19330"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOX69998.1"
                     /translation="MTNRTPLPPGFMVVHGNHPESLRDLMLSWMTRHPLAPLEDEWVL
                     VQSNGVAQWLKLSLAAPVAQGGCGIAAALRTELPASFIWQAYRAVLGNGSVPAVSPFD
                     KPMLVWRLMRLLPVLLAEEAFVPLRRFLAQDDDRRKLHQLAERLADLFDQYQVYRADW
                     LADWAGGRDVIATSREGSQPLPDEMRWQPRLWRALLDDVGPHAAASSRADVHRRFLDQ
                     AHGWHGDRPAGLPRRLVVFGISSLPQQSLEVLATLARWMQVLMCVHNPCEHDWSHVVA
                     DKDLLRAQRLRQRRRPGGEGEIADEALHLHAQPLLAAWGKQGRDFIRLLDAHDEREAY
                     APRFAAIGGRVDCFERNRGATLLEQLQDDIRDLRPLAETRNTWPPLQLVADRSIRFHI
                     AHGPQREVEVLHDQLLAAFAADPTLQPREVIVMVPDIAAYAPHVQAVFGLLQRDDARH
                     IPFTLADRGQRHHDPLLGALEKLLGLPQCRLAVSDVLDLLEVPALRARFGIAEPQLPL
                     LHRWVQAANARWGLHAEQRGRLGLPADLAQNTWDFGLQRMLLGYAVGSGEAWEGIEPM
                     DEVGGLDAALLGPLSRLLGALEHHWRALDEPATPTAWGERLRALLQQFFLAGAGTDGF
                     TLLRLDKALQDWQDACQAAALDEPLPLSVVREHWLTQIDAGSGVNQPFFAGAVTFATL
                     MPMRAIPFRFVALLGMNDGDYPRSRPPTDFDLMAKDWRPGDRSRREDDRYLFLEA"
     gene            complement(2353..>3269)
                     /locus_tag="PKV56_19335"
     CDS             complement(2353..>3269)
                     /locus_tag="PKV56_19335"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HOX69999.1"
                     /translation="GATYHRSATARDRDMLREQVLRGLGWEILRVWSTDWWVDRVGTL
                     EKIDRKLRDLLVLSRERRAVEEQRVAAAEEAAAAIARASADAAHSSGVDPAGEADNEL
                     AGLSADTGRTPPAQATRLPVETYARNAGASEVAATQFVEADPKSAVEGVSADDFFNKT
                     YDKVLLPMINHVVEVEGPVLDAVLARRIARAHGWQRTGARIHERVVGLAALSHRGTSE
                     EVGTFFWPSGLEPGAVIAFRRAADDSARSVDEVCLPELIALARGVLSGGLTTEAAVVA
                     MARELGLQRLRAASRARFEAAIARAHDS"
BASE COUNT          555 a         1154 c         1068 g          492 t
ORIGIN      
        1 gcttccagga acaggtagcg gtcatcctcg cggcgcgagc ggtcacccgg ccgccaatct
       61 ttggccatca ggtcgaagtc agtcggcggc ctgctgcgcg ggtagtcgcc atcgttcatg
      121 cccagcagag cgacgaagcg gaacgggatc gcccgcatcg gcatcagcgt ggcaaacgtc
      181 accgcaccag caaagaaggg ttggttgacg ccgctgccag catcgatctg tgtcagccag
      241 tgttcgcgca ctacagacag cggcagcggc tcgtccagcg ctgcggcctg gcaggcgtct
      301 tgccaatctt gcaatgcctt gtccaaccgc agcagtgtga agccgtcggt cccggcccct
      361 gcgaggaaaa actgttgcag caatgcgcgc agccgctcac cccaggcagt gggcgtggca
      421 ggctcgtcca gcgcgcgcca gtggtgctcc agcgcgccga gcaaccggct cagcgggccc
      481 agcagcgcgg cgtccagccc gcccacctcg tccattggct cgatgccctc ccaggcctcg
      541 cccgacccca ctgcatagcc cagcagcatg cgttgcaggc cgaaatccca ggtgttctgc
      601 gccaggtcgg ccggcagacc cagccggccg cgctgctcgg cgtgcagacc ccatcgcgcg
      661 ttggcggcct gcacccaacg gtgcagcagc ggcaactggg gctcggcgat gccgaagcgc
      721 gcgcgcagtg ccggcacctc cagcaggtca agcacatcgc tcaccgccag ccggcactgc
      781 ggcaggccca gcagcttttc cagcgcgccc agcagcggat cgtggtggcg ctgaccacgg
      841 tcggccagcg tgaacgggat gtggcgcgca tcgtcgcgct gcagcaggcc gaacactgcc
      901 tgcacatgcg gcgcataggc ggcgatgtcg ggcaccatca cgatcacttc gcgtggctgc
      961 agcgtcggat cggcggcaaa cgccgccagc aactggtcgt gcaacacctc cacctcgcgc
     1021 tgcggaccat gcgcgatgtg gaatcggatg gagcgatccg caacgagctg cagcggtggc
     1081 caggtgttgc gtgtttctgc cagcgggcgt agatcgcgga tgtcgtcctg cagctgctcg
     1141 agcagcgtgg caccgcggtt gcgctcgaaa caatccacgc ggccgccgat ggccgcaaag
     1201 cgcggggcgt aggcctcgcg ctcgtcgtgc gcgtccagca ggcggatgaa gtcacgcccc
     1261 tgctttcccc aggccgccag cagcggttgg gcgtgcaggt gcagcgcctc gtcggcgatc
     1321 tcgccctcgc cgcccggccg gcgtcgctgg cgcagccgct gggcgcgcag caggtccttg
     1381 tcggccacca cgtgcgacca gtcgtgctca caggggttgt gcacgcacat cagcacctgc
     1441 atccagcgcg ccagggtcgc cagcacctcc agcgattgct gcgggagcga cgagatgccg
     1501 aacacaacca gccgacgcgg caggcctgcc ggccgatcgc cgtgccaacc gtgggcctgg
     1561 tcgagaaagc gccggtgcac atcggcccgg ctgctggccg cggcgtgcgg tcccacgtcg
     1621 tccaacagcg cacgccacaa ccgcggttgc caacgcatct cgtcgggcaa tggctggctg
     1681 ccctcgcgcg aggtggcgat gacgtcgcgc ccgcccgccc agtcagccag ccagtcggcc
     1741 cggtagacct ggtactggtc gaacagatca gcaagccgct cggccaactg gtggagcttg
     1801 cgccgatcat cgtcctgcgc cagaaagcgg cgcagcggca caaacgcctc ttcggccagc
     1861 agcaccggca gcagccgcat cagccgccac acaagcatcg gcttgtcgaa gggcgagacc
     1921 gccggcacgc tgccgttacc cagcactgcg cgataggcct gccagatgaa gctcgccggc
     1981 aactcggtac gcagcgctgc ggcgatgccg catccacctt gcgccaccgg tgcggccagt
     2041 gacagcttga gccactgcgc cacgccgttg ctctgcacca gcacccattc gtcctccagc
     2101 ggcgccagcg ggtgccgtgt catccaggac agcatcaggt cgcgtagcga ctcggggtgg
     2161 ttgccgtgga ccaccatgaa gccggggggt aagggagtgc ggttcgtcat tgatcaattg
     2221 ctcttcgacg tggtgttggc ttggatgttc gacgatggct agctcacgga gtgagcctgc
     2281 ttgccgtggc caaggcggct ccgtgcagtg ccacgttcag cgactggacg gcaagcacat
     2341 caagctggag ggtcaagagt cgtgtgcgcg tgcgatggcc gcttcaaacc gcgccctgct
     2401 ggcagctctc agccgctgca ggcccaactc tctcgccatc gccacgacag cggcttcggt
     2461 ggtgagacca ccggacaaca cgccacgagc cagagcaatg agttcaggca ggcaaacctc
     2521 atccacggac cgtgcgctgt cgtcagcagc gcgccggaat gcgatcactg cccccggttc
     2581 cagaccggac ggccagaaga aggtgccgac ctcctcagag gtgccacgat gcgacaaagc
     2641 cgccaggcca acaacgcgct catggattcg cgcaccggtg cgctgccagc cgtgtgccct
     2701 ggcgatgcgg cgcgccagca cggcatccag cacagggccc tcgacctcca cgacatggtt
     2761 gatcatgggc aacaggacct tgtcgtaggt cttgttgaag aagtcatcag ccgagacccc
     2821 ttccaccgcc gatttcggat cggcctcaac aaactgcgtc gctgcgacct cagaagcacc
     2881 agcgttgcgg gcataggttt cgaccggtag ccgggtcgcc tgggcaggcg gtgtgcggcc
     2941 ggtgtcggcc gagaggcccg caagctcgtt gtccgcctca ccagccgggt cgacaccgga
     3001 ggaatgcgca gcatcggcgc tggcgcgggc aatggctgcc gccgcctctt cggccgctgc
     3061 cacgcgctgt tcttccacgg cccgccgttc ccggctgagg accaacagat cccgcagctt
     3121 gcgatcgatc ttctccagcg tgccgacacg gtcgacccac cagtctgtcg accacacgcg
     3181 gaggatctcc caacccaggc cgcgcaggac ctgctcgcgc agcatgtcgc ggtcccgggc
     3241 cgttgcggag cggtggtagg tcgcaccat
//
LOCUS       DAOORV010000291         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_35264_length_3258_cov_8.089291, whole genome shotgun sequence.
ACCESSION   DAOORV010000291 DAOORV010000000
VERSION     DAOORV010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35264_length_3258_cov_8.089291"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            495..1538
                     /locus_tag="PKV56_19340"
     CDS             495..1538
                     /locus_tag="PKV56_19340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein D"
                     /protein_id="HOX70000.1"
                     /translation="MEAIVRAIDVGFGNTKYVVAAQGGRVECAHFASIAYDSAHDNTG
                     DALGSKRKTVCVPVGGMWYEVGPDVALAADGFVGRNHHDDYMRTHEYRAFTAGALHFM
                     KVDRVDLLVLGLPVADYMRRRAELQKAMTGTFEVGRNRKVEVKRVLVVAQPQGAMYAV
                     FGDQANAKLRQGRTLVIDAGSRTFDWLVTLDSKVVGKMSDSVNRGVYDILVKIAAAVA
                     SETGRGYQNLEAIDKALRSRKPLRAYKRSVDLKKYDNAIEKIADQAVAMLHGKLGSTD
                     DLEHIVVVGGGMHLYRASIRRKFPEIAISEVDEPLYANVKGFQLMGEQYVRENPQLFA
                     TAPARVDAAPVGA"
     gene            1857..2303
                     /locus_tag="PKV56_19345"
     CDS             1857..2303
                     /locus_tag="PKV56_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70001.1"
                     /translation="MNRTDRDLVSASELAQLGYCERVAHFDWRYGARRSHEQLRAQDR
                     GNAAHDQFYKDSLAIARESERKGRCFVATLALGECAETRALRAFRDLYLRRTAQGRWF
                     IGTYYRLGPMVCDLLSRHPRLLSAVRTVVVLAGRLAARAVARRLTK"
     gene            2300..2848
                     /locus_tag="PKV56_19350"
     CDS             2300..2848
                     /locus_tag="PKV56_19350"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOX70002.1"
                     /translation="MSDVSAGALLLLGLLLFAAAYSWLRTQRERPVETWLPREIAEGE
                     LAFSEKRFESKRLGLVARLDRAYRKGGELHLVELKTRGYFAAHASDAIELSVQRLVLQ
                     EATGERVSSVAYVAVQQHGEGQPRPIRVALFDEDEVLAMRRRLLELRRGLGHGPSPAA
                     RRKACDKCGHRAVCQSTFGDRQ"
     gene            2997..>3258
                     /locus_tag="PKV56_19355"
     CDS             2997..>3258
                     /locus_tag="PKV56_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70003.1"
                     /translation="MYITDLTHFLDGSGAIAPIKGPARAMAQFVVEVVAHATDESDTS
                     PAAPTCFKCKKGPVTALRAADDAVVWKCPKCEAEGRISKWQGT"
BASE COUNT          616 a          957 c         1157 g          528 t
ORIGIN      
        1 ccgcaacacc ggcctgcttg cattgagagc tgtggcaggc agcacaggct cgggaagaag
       61 ttgaacccat gcgagggcag gttgtcaaca ccggcagggc ttgaacaatc gcgatgctct
      121 ggcgttggtt agtgtgtcgc tggcgttgcc gaaagcgaac tcctctcagg cttgacttcc
      181 ttgcctcccc gtctagatct cgaaggtccg tgtaacggca agcacgagag aaagtgtcca
      241 cctccgaacc aggacgagaa ccgcggccaa ggcgcgccga aggactgccg atgccgccac
      301 gtatctagga catcagagcc gcgggaccgg aggtgcctca gtggatgtcc gtcgcaagtg
      361 gcgcgtcgaa accgcgcggc gcgccgcagg gcgaaaaaaa gctggggggt gcgaccggcc
      421 tttccgggtt ggcggcacgt ggactcttgg cgatgctgtc gccatcggtc aatgcaatat
      481 ggagagcacc gtccatggaa gcaatcgtca gagccatcga tgtgggtttc gggaacacca
      541 agtacgtggt ggccgcgcaa ggcggaaggg tcgagtgcgc ccacttcgcc tcgatagcct
      601 atgacagtgc gcacgacaac acgggcgatg ccctcgggag caagcgcaag acggtctgcg
      661 tgccggtggg cggcatgtgg tacgaggtgg gccccgacgt tgcgcttgcg gctgacggct
      721 tcgtgggtcg gaaccatcac gacgactaca tgcgaacgca cgagtaccga gccttcactg
      781 ccggggcgct tcatttcatg aaggtcgaca gggtcgacct gctggtgctc gggctgccgg
      841 tggcggacta catgcgccgt agggcggaac tgcagaaggc gatgacggga acattcgagg
      901 tgggccgcaa tcgcaaagtc gaggtcaaac gggtgctggt cgtagcgcaa ccccaaggag
      961 ccatgtatgc ggtcttcggg gatcaggcga acgcgaagct taggcaaggc agaaccttgg
     1021 tcatagacgc tggctcgcgc acgttcgact ggctggtgac gctcgacagc aaggtggtcg
     1081 gcaagatgag cgactcggtc aaccgagggg tgtacgacat actggtcaag attgcggccg
     1141 ctgtcgcgag tgagacaggc aggggctacc agaacttgga ggcgatcgac aaggctttga
     1201 ggagccgcaa gcccctgcgc gcctacaagc ggagcgtcga cctcaagaag tacgacaacg
     1261 cgatcgagaa gatcgccgac caagctgtcg cgatgctcca cggcaagttg ggatcgacag
     1321 acgacctgga gcacatcgtg gtggtcggtg gcggaatgca cctgtaccgc gcatcgatcc
     1381 ggcggaagtt ccccgagatc gccatcagcg aggtggacga acccctctac gcgaacgtca
     1441 aagggttcca gctcatgggc gagcagtacg tccgtgagaa cccgcagttg ttcgccaccg
     1501 cacccgcccg agtcgacgcg gcccctgtcg gagcgtagcc atgggagccc agccgaaatt
     1561 ggtgatgcag gtgaccgtca gcgcggaaga gtgccccgag ctccatcgag agctcattgg
     1621 aatgacagct gctcgaaggc gcgtgaagcg gcttcttgga ttggcgagcg tcgggctcct
     1681 ggtcgaacag gggcgcatcg gtgacgcggc gatctcgagc cggggccact cagcgccgcc
     1741 gatctcgatg tcgctgcggc cgctggggca cgcagaccct gtaccgctgg cggggctccg
     1801 aggggccgtc gttggccaga gcatctccga gatgaccgac gcgttcgggg aagtggatga
     1861 ataggactga tcgagatctg gtcagcgcca gtgagttggc gcaactcggc tactgtgaac
     1921 gcgtggcgca cttcgactgg cgctacggcg cgaggcgctc gcacgagcaa ctcagggcgc
     1981 aggatcgtgg gaacgcggcg cacgatcagt tctacaagga cagcctggcc atcgcgcgcg
     2041 agagcgagcg gaagggccgg tgcttcgttg cgaccttggc gcttggcgag tgcgccgaga
     2101 cccgtgcgct acgagcgttt cgggacctct acctgcggcg cacagcgcag ggccggtggt
     2161 tcatcgggac gtactaccgc ctcggaccca tggtgtgtga cctgctgagc cggcatccgc
     2221 ggttgctgtc ggcagtgagg accgtcgttg tcctggcagg ccggttggcc gcacgggcgg
     2281 tagcgcgaag gctgacgaaa tgagcgacgt cagcgcgggc gcgctgctcc tgctggggct
     2341 gctgctcttt gcggcggcat acagctggct gaggactcag cgggagcggc cggtcgagac
     2401 ttggctgccc cgagagatcg ccgaaggtga gctggccttc tcggagaagc gcttcgagtc
     2461 gaagcgcctt ggtctggtgg ccaggctcga ccgcgcgtac cgcaagggcg gagagcttca
     2521 cctggtggag ctgaagacgc gcggctattt cgcagcgcac gcctcggatg cgatcgagct
     2581 ctcggttcaa cgtctggtcc tgcaggaggc gaccggcgaa cgagtgtcat ctgtggctta
     2641 cgtggcagtc caacagcatg gcgaaggaca acctcggccg atccgggttg ccctgttcga
     2701 tgaggacgaa gtcctggcca tgcgccgcag gctactcgag ctgaggcgtg gcctgggcca
     2761 cgggccatcg ccggcggcgc gccggaaggc gtgcgacaag tgcgggcatc gagccgtatg
     2821 ccaaagcacc ttcggagacc ggcagtagcg gcccgttgct ctgcggcagc accggggcaa
     2881 agtatggacg ggaggtctcg aaccgcatcg accggcaggg acgtgctgcc atgagtgcga
     2941 agccgggggg aggcgaccac ctcgctggct agacttggcg cacttccgag cctccgatgt
     3001 acatcaccga cctcacacac ttcctcgatg ggtccggcgc gatcgcgccg atcaagggcc
     3061 cggcgcgcgc gatggcgcag ttcgtcgttg aggtcgtcgc tcacgccacg gacgaatcgg
     3121 acacctcgcc ggcggcgcca acatgcttca aatgcaagaa gggtcccgtc accgcgctgc
     3181 gggccgcgga tgatgccgtc gtgtggaagt gcccgaagtg cgaggcagag ggcaggatct
     3241 cgaagtggca ggggacgc
//
LOCUS       DAOORV010000292         3253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_35335_length_3253_cov_11.523765, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000292 DAOORV010000000
VERSION     DAOORV010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35335_length_3253_cov_11.523765"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKV56_19360"
     CDS             <1..368
                     /locus_tag="PKV56_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70004.1"
                     /translation="RERTGLLANTLGWPVLSAGLGLLVFAGAGRRSLIGRWPVPGMAW
                     LAAISYSLYLSHKIALHVVHVTLAPSLEGSRVLMFLSYALAVLLLGALLHYSVERPFL
                     RWRDRRQGTRLTAASPSAG"
     gene            1104..2759
                     /locus_tag="PKV56_19365"
     CDS             1104..2759
                     /locus_tag="PKV56_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70005.1"
                     /translation="MTEFDAAVSPASAPAPVADSTSADLTPQPPAESRAAPQPPQRVA
                     FRLSYCTDTSSRLRIEHSGLLLLRDRRAAVKIWQGALRRLERLMTRCMRSFRHDRQAV
                     ILVHGIGEQRPGQLLREFISNVFDHRAGERYYIKPDLVSSLFEMRMASVPRIDGQRPT
                     TDVYELYWAHLIRDTTITQVCGWMLRLLLSKDTKIPQTLLRLVWIVRVVLVVALALVA
                     WTATKDISGWLKVFAGGAFVALPSLLAIGMKALRDEYLVNFAGDAARYLEPRADNVSR
                     RQEIREAGAKLIEALHEKRRYERIVVYAHSLGSVIAYDILSNAWARHSRTRDPVARTS
                     SRALRALEDLLNKHSCPQPAPDIDAVQGMQHAAWAEYRHNGFQWRVTDFVTVGSPLAH
                     ARWLLNLDVKTEFADLVRERSLPTCPPVTEEINGPAKSEKRKVFTFTHAYADPDNSRA
                     KRSVQVPHHGGLFALTRWTNLFFPYRGLVDGDPVAGPVAPCFGHWVKDVSLRLTKGFA
                     HCRYTDRSLEPQAVEQVRLALNLPMRRPLAEHLPAKLHPTVLQ"
BASE COUNT          570 a         1048 c         1022 g          613 t
ORIGIN      
        1 ttcgcgagcg caccgggctg ctcgccaaca cgctgggctg gccggtgctg tcggcagggc
       61 tcgggctgct ggtgtttgcc ggggcgggcc gccgcagctt gatcggccgc tggcccgtgc
      121 cgggcatggc ctggctcgcc gcgatctcct acagcctgta cctgagccac aagatcgcgc
      181 tgcatgtcgt gcacgtgacg ctggcaccgt cgctggaggg ttcgcgcgtg ctgatgttcc
      241 tgagctacgc gctggcggtg ctgctgctcg gcgcgctgct gcactactcg gtcgagcgcc
      301 cgttcctgcg ctggcgcgac cggcggcagg gcacgcgcct tactgccgcc tcgccctccg
      361 ccgggtagtg cgggcgtctc agaaaaccaa gcgattggtc ctgccgctcc cggccacaag
      421 cggtccctcg ttgcttcgcc tgacacccga cagtccgcgg acgcgcagca agggtttgac
      481 gtcaaccagg gactgtggcc ctcgccaaat gacgtgctga tcgccttggc ggtcaattct
      541 cgaacgaaag gcggagggat cagtgccttt gcggcaaggt ggcgcacggc caacgtttcc
      601 agcgagcgtc agggatgccg ccaggctgcg agcacgagcc gccccaacgc cgccacaatg
      661 gtgcgtgccg atcaacttcg acgctaggca gtacacagca ccagcgtgcg tcctcacggc
      721 tttcagatgc atttgaacgc aacggtagaa cggatcgctg gatcccgagc gagtcgggtg
      781 gcaagatggc acgcaggcaa tcaagccggg tccgggagac tgacgctatg tccgcatgcg
      841 aggcgcctgg ggcgacatcc atccgcgttg cgctaaacct gccttttcct gctgccgagg
      901 cggccgcgtg tctgttgttc cccgaggttg cggcctactg gatcggacga gactgcaact
      961 tgagctcgca gttgttcgag cgcgtgcatc ttccgatgtg gacgttctcg cagtccggtc
     1021 gagtccaatt cgatgacttg cgaagcggca gagttgttga gtccgattgg tctgtcgcag
     1081 tcgagggcaa ggcttactac ctggtgaccg agttcgacgc tgcagtttct cccgcatcag
     1141 cgcctgcgcc ggttgctgat tccacttcag cagatctgac gccacagccc cccgcggaaa
     1201 gccgtgctgc gccacagcca cctcagcgcg tcgcgttccg tctctcgtat tgcaccgata
     1261 ccagcagtcg gctgcgcata gaacacagcg gtttgctgct tcttcgcgat cgcagagccg
     1321 ccgtgaagat ttggcagggc gcgctgcgcc ggctcgagcg cctcatgacc cggtgcatgc
     1381 gcagctttcg acatgatcgg caggccgtca tcctggttca cggcatcggc gagcaacgac
     1441 ctggtcagct gctgcgcgaa ttcatctcca acgtcttcga ccaccgagcc ggcgagaggt
     1501 actacatcaa accggatctt gtctcctccc ttttcgaaat gcgcatggcc agcgtcccgc
     1561 gcatcgacgg gcagcgtccg acaacggatg tctacgagct gtactgggcg caccttatcc
     1621 gcgacacgac gattactcag gtttgcggct ggatgctgcg ccttctcttg tcgaaggaca
     1681 caaagatccc acagacgctc cttcgattgg tttggatcgt gcgcgtggtg ctcgttgtgg
     1741 cgcttgcgct ggtcgcgtgg acggcgacca aggacatctc cggctggctc aaggtctttg
     1801 caggcggagc cttcgtcgcg ctgccaagtc tgctggcgat cggcatgaag gcactgcgcg
     1861 acgagtacct cgtcaacttc gccggagatg cggcccgata tctggagccg cgcgctgaca
     1921 acgtttccag gcgacaggag attcgagaag ctggcgccaa gctgatcgag gcgctccacg
     1981 agaagcgccg ctatgagcgc atagtcgtct acgcgcatag cttgggcagc gtcatcgcct
     2041 acgacatcct tagcaatgcc tgggcacggc attcacggac gcgcgacccg gtggcgcgca
     2101 ccagcagcag ggcgttgcgc gccctggagg atctgttgaa caagcattca tgcccccagc
     2161 cggcacccga catcgatgcc gtccagggca tgcagcatgc ggcgtgggcc gagtaccggc
     2221 acaacggctt tcagtggcga gtcaccgact tcgtgacggt cggctcgccg ctggcccatg
     2281 cgcgctggct gctgaatctg gatgtgaaga cagagttcgc cgacctggtt cgcgagcgct
     2341 cgttaccaac ctgcccgcct gtgacggaag aaatcaatgg ccccgcgaag tcggaaaagc
     2401 gcaaggtctt cacgttcacc cacgcgtatg ccgacccgga caactcgcgc gcaaagcgat
     2461 cggtccaggt tccgcaccac ggaggtctgt tcgcgctaac gcgctggacc aatctcttct
     2521 ttccctaccg aggcctcgtc gacggcgatc cggtggcggg cccggtggcg ccgtgctttg
     2581 gccactgggt taaggatgtc agccttcggc tcaccaaggg gttcgcccac tgccgctaca
     2641 cggatcgatc gctcgaaccg caggcagtcg agcaggttcg tctcgcgttg aatctgccga
     2701 tgcgcaggcc gctcgccgag catctgccag ccaagctgca tccaacggtc ttgcagtagg
     2761 aaccggtgct gccagtgcgc cggagcttca tgccgggcgg ggcatgaaac agaagagtgc
     2821 gtgactcgcg gctacagctg gcagcgacgg cagtgtagac gaacgcgcac tggcttcgac
     2881 agcctcagtg tcaagcgttc tcacacggcc tcagccgagt ccgggatcgc gctgatgtcg
     2941 ccgcaagttg accttggccg gcgggtcggt cgaatcgaac gtcaggttcc tcgcacggac
     3001 tcgcactcac acattcggcc aggagctgtc gtcaacgaac gtctgcttga aggtgtgcat
     3061 caaccaccgt ccaacggcat cgcattcaat gccttgcatc cgccttggcg agttccacca
     3121 tgcacgcctg cattgtgaaa ccattgggac ggcgaccaag cggctacagc cgggcgaatc
     3181 aagttctgcc accgatgccg acatcgacta gggagtgaca ggcgccatat cgagcactcg
     3241 aatgctggcg cag
//
LOCUS       DAOORV010000293         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_35732_length_3225_cov_7.438486, whole genome shotgun sequence.
ACCESSION   DAOORV010000293 DAOORV010000000
VERSION     DAOORV010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35732_length_3225_cov_7.438486"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            816..1343
                     /locus_tag="PKV56_19370"
     CDS             816..1343
                     /locus_tag="PKV56_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70006.1"
                     /translation="MLTNKNEVLAAALDQTNTWLAQTLGRPDLQLQLISQPIARATAM
                     QFERGGGLRRKVHWNWEEIFFSRVRHQDAWMYAMVRAGRPRAMCYGRIQVKGAYVSLE
                     YLERAPNARGIRGFTAPTAFQFARAVALLLDLTEVRLNEPFPHLVQYYERVLEGAIRH
                     PAAGPVKYLLVKVST"
     gene            1340..1621
                     /locus_tag="PKV56_19375"
     CDS             1340..1621
                     /locus_tag="PKV56_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70007.1"
                     /translation="MKRVRVAPGKFVVVSQGMAEKAARVFATGAFTRDQVREMAAAEP
                     KGASGVFLGKATAKRAGAVSGKTGTFVRQTTGHVVVAGVDRAPTKRKAA"
     gene            complement(1782..3059)
                     /locus_tag="PKV56_19380"
     CDS             complement(1782..3059)
                     /locus_tag="PKV56_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 domain-containing protein"
                     /protein_id="HOX70008.1"
                     /translation="METSAHAITAPSAIYEIGGRRFEIGSRGFSDAIAEAHASQQRPR
                     CMCLVDGVEMYVARLAGSDRGYIVKRMPDTGSHHAPDCPSYEPPAEFSGLGQVLGSAI
                     TEDPATGETTLKLDFPLTKMPGRSTIPPTGGEGDSVSSTGTKLSLRGLLHYLWDQAEL
                     TRWHPGFAGKRTWATVRRHLLHSAEHKLARGDTLRARLYVPEPFSIEERDAINARRLA
                     QWQSAVPSPGKAQQLMLLICEVKEIVPARYGFKAIVKHMPDQAFAIDEQLYRRLGRRF
                     EAELALWGASDDIHMVMIATFGVSSAGVPAIQELCLMPVTRQWLPVEDGFEKQLLERL
                     VADGRSFVKGLRYHLSRARGLANATLTDCDGPARTLFIVTADSDPLLDGLDTDMKSNA
                     QWVWRRSIDPMPVLPQRSHRRESDGSSATASGG"
BASE COUNT          558 a          997 c         1100 g          570 t
ORIGIN      
        1 cgctaggggg caacctcaat ggatgtgaca gtccctcgcc gaatcaggcg gcgagagacc
       61 atttgggccg ttcgattcgg ccagggcgac cggaatccgg aggaagaagc gtgggcagcg
      121 ccaggacggc agactgaatc gcagcaaccg aagcggtctg actggcacag ggttgcacgc
      181 aacccagatg gcaaaacgca cagtccaggt cgtctgcaag caccgaggac tttttggcag
      241 cggagcctga atcggactcg gctttcgctt ggtgctgatg gctgtggtgg ccgaagtgcc
      301 ctgaacttgg agccttctcg tggccgcagt aggtcgctgc cgcaccccag gcaaactgga
      361 gcggcagaac ggccagcaga aagagaagca caaagcgacg cacgagatga gtttagcgga
      421 ctcggacgaa acgctgctcg ccgagcctcg cggcggacat tccggtggag gtcaaacgcc
      481 cctttggcgc gacatgttgc cggtttcgtt gtgaccacat cccgtcttcc tcgtcggatc
      541 ttgcacagtt gagcgtgtcc cagacgcttt gagatgtcgc ggaggactcg gcgctggtgg
      601 atgtcccgcg ggccactccg cgcgcatccg ggcgagtgaa gtggggcgcg attgagcctg
      661 atgacagtga gcaagcggtc cgtgatcggc tcgccggcga agtcgggcaa ctgggtgcag
      721 ttgggccacc ggctccgagt ggtgcgtacc aacatgggct tgttgcgcgg tggggatggc
      781 cggtcaccgc tgcggtgcgc gatgattgcg accgcatgct tacgaacaag aatgaggtgc
      841 tggccgcggc gctggaccag accaatactt ggcttgccca gactctagga aggccggacc
      901 ttcaactcca gctgatcagc cagcccatcg ctagggcgac tgccatgcag ttcgagcgag
      961 gaggcgggct caggcgcaag gtgcactgga actgggagga gatcttcttt tcccgagtgc
     1021 ggcatcagga cgcctggatg tacgcgatgg tgcgtgcagg ccgcccgcgc gcgatgtgct
     1081 acgggcgcat tcaagtaaaa ggggcctatg tgtcgcttga atacctcgag cgcgcgccca
     1141 acgctcgcgg gatccgcggc ttcacagcgc cgaccgcctt ccagttcgcc cgcgccgtgg
     1201 ccttgctgct cgacctcacg gaggtgcggc tcaacgagcc gttcccgcac cttgtgcagt
     1261 attacgaacg ggtgctggag ggagcgattc gacaccctgc ggcgggtccg gtaaagtacc
     1321 tgttggtgaa ggtctcaaca tgaagcgcgt gcgagttgcc ccaggcaagt ttgtcgtcgt
     1381 ctcccagggg atggcggaaa aggccgcgcg cgtgtttgcc acgggcgcgt tcacccggga
     1441 tcaggtgcgc gagatggctg cggccgagcc taagggtgcg agcggagtgt tccttggcaa
     1501 ggccactgcc aagcgagcgg gagccgtgtc gggtaagact ggaacgttcg tgcgccagac
     1561 cacgggccat gttgtggtgg ctggcgtcga ccgcgcaccg accaagcgca aggctgctta
     1621 ggtccgcaac ccagccggct ggcggttctc cgggtggcaa agcccgctac gagttgacat
     1681 aaacattgtc ggcgtgctgc agagcgcctg ttgtccgaac cttgcatgac gtcgcagcca
     1741 ccagagtgca gtcgctgcaa cctcttcgtt ccagcggtgg ctcagccgcc tgaggcagtt
     1801 gcactcgaac cgtccgactc gcgacgatga gatcgctggg gaagtacagg catcgggtcg
     1861 atcgatctcc gccaaaccca ttgtgcattg gacttcatgt cggtatccag gccgtccaac
     1921 agcgggtctg agtcggcggt cacgatgaac aaagtccttg ccggcccgtc gcagtcggtc
     1981 agagtcgcat tggccagccc gcgcgcacga ctcaggtggt agcgaagacc cttcacgaag
     2041 gatcgcccat cggcgacgag tcgctctaag agctgcttct cgaagccatc ttcgaccggc
     2101 agccattgcc gcgtcactgg catgaggcac agctcctgga tcgccggaac gccggcactg
     2161 ctgacgccga aggtcgcgat catcaccatg tggatgtcgt cgctggcacc ccacagcgcg
     2221 agttcagcct caaagcgccg gccgagccgc ctgtagagct gctcgtcgat cgcgaaggcc
     2281 tggtcgggca tgtgcttcac gattgccttg aacccgtagc gtgccggcac gatctccttg
     2341 acctcgcaaa tcagcagcat cagctgctgc gctttcccgg gagacggcac tgcgctttgc
     2401 cactgggcca ggcggcgggc gttgatcgcg tcgcgctctt cgatggagaa cggttccggc
     2461 acgtagagcc gagcgcgcag ggtgtcgccg cgggcgagct tgtgctcggc tgagtggagc
     2521 aggtgccttc gtaccgtggc ccatgttcgc tttccggcga acccgggatg ccagcgcgtc
     2581 agctcggcct ggtcccacag gtagtgcagc aggccgcgca gcgagagctt ggtaccggtg
     2641 gaggagacgc tgtcgccctc tccgccagtg ggagggatcg tcgaccggcc cggcatcttg
     2701 gtcagcggga agtccagctt gagcgtcgtc tcgccggtgg ccgggtcttc ggtgatcgca
     2761 ctgcccagca cctgccccag gccggagaac tcggccggcg gttcgtagga cgggcagtcg
     2821 ggcgcgtgat ggctgcccgt gtcgggcatg cgcttgacga tgtagccccg atccgaaccc
     2881 gccaggcggg cgacgtacat ctccacgcca tccaccagac acatgcagcg agggcgttgc
     2941 tgcgaggcat gggcttcggc aatcgcatcc gaaaacccgc gcgacccgat ctcgaagcgc
     3001 cggccgccaa tctcgtagat cgcggatggc gccgtgatgg catgggccga cgtttccacc
     3061 ggcgtggcct caccgcacgg gcgctggcgc agcccggtcg cgcgtgcgct gcatctcggg
     3121 cgtcggtact accgggcgct gcgacggcgc agccttgtcg tagatcttga ccttgggcac
     3181 ctggccgtcg cggatgcgct gggccagctt ctcggtcgcg gcggc
//
LOCUS       DAOORV010000294         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_36388_length_3182_cov_10.419571, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000294 DAOORV010000000
VERSION     DAOORV010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36388_length_3182_cov_10.419571"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..852)
                     /locus_tag="PKV56_19385"
     CDS             complement(229..852)
                     /locus_tag="PKV56_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX70009.1"
                     /translation="MVTTSKKSKRTAPQAVGELPLLSLARQVRSWTDTLLGVAGSAGD
                     IGMTLAQARIRDPEKKAAVGKAGSQLRQWREEAGLTAKELSDAVGLGDTKLIEQAEGG
                     TAALPFEIVLRLAGVLGRRDPIPVAMGLTRQYNPELWKALESIGIGRLAVQGTREREL
                     ANVYRGNDAARALDDETFAQVLAFTKQAFDMAVDFSRRPARTKAKPG"
     gene            complement(854..1471)
                     /locus_tag="PKV56_19390"
     CDS             complement(854..1471)
                     /locus_tag="PKV56_19390"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX70010.1"
                     /translation="MSTVTRELNPTERASLLAHLLAMPAEDRRLRFAHAIADEGLQRY
                     VEGIDFARDTVFVATDAELNIIGAAHLARDGEHAELGVSVLAPARGQGIGAALLERSA
                     THARNAGVRVLFMNCLVENAPMLHLARKQGLQVAVSSGEAEAFVRLPRPSLRSLASEA
                     VADHLGLIDHAHKAHWVALRGLWLASFGGESEPPRGGSETQEGSS"
     gene            complement(1482..1808)
                     /locus_tag="PKV56_19395"
     CDS             complement(1482..1808)
                     /locus_tag="PKV56_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70011.1"
                     /translation="MRLILLGPPGAGKGTRDLAASATSLFPPPLIATKDEVTGEELVQ
                     RGDDTEGTLRKRLVVYRTQTQPLLAYDAAWDATGDRNAPRCRHVHGLGSVDQVAALAL
                     AAVANQ"
     gene            complement(1805..2359)
                     /gene="ppa"
                     /locus_tag="PKV56_19400"
     CDS             complement(1805..2359)
                     /gene="ppa"
                     /locus_tag="PKV56_19400"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOX70012.1"
                     /translation="MNLENLPIGSDPPHEVNAVIEVPIGGEPIKYEMHKGAGMLVVDR
                     FLYTPMRYPGNYGFIPHTLSEDGDPCDVLVANTRPLMPGSAIAVRPVGVLRMTDEAGG
                     DEKIVAVPATRLTQRYAHVHNYTDLPEIAWRQIEHFFAHYKDLEPGKWARFEGWGDAD
                     LAKELIQRAIARAAKIKGVAEALR"
     gene            complement(2361..2762)
                     /locus_tag="PKV56_19405"
     CDS             complement(2361..2762)
                     /locus_tag="PKV56_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70013.1"
                     /translation="MPFNHCWEPTGIVTTYRGDLVLEEVLESHQRMAADPRFDDLRFM
                     IVDTLPVERVALIASDAVQINAFLTGPALTNPYTFVVFIALHPAVLRFVAVYAQLPDR
                     RYDLRVCSSMVSARQLLAHRDSRAIQGMRGA"
     gene            complement(2888..>3182)
                     /locus_tag="PKV56_19410"
     CDS             complement(2888..>3182)
                     /locus_tag="PKV56_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX70014.1"
                     /translation="GNLVGSLIGMSIASIGISTMTPMFFTITSEYLSKAAAAGGIALI
                     SSLGNLGSAASPAVAGALTKSTGNPISTMYMVLVMFILAGLIVMFAVRPYKER"
BASE COUNT          518 a         1068 c         1034 g          562 t
ORIGIN      
        1 ctagagtttt tctgggtcac cgtgtcaatc tgcaggaaaa atacgattac cccttcgagg
       61 aacttcgtcg gccgcggacg tgcaaggctg ttggcgtcgc gcgcacatcg gtgcgctgcc
      121 tgcagcccag tggcgcagca aagttggcgg cccttcggca gatggcagcg aaacggccaa
      181 gcgattccga caggccgaag cggccattgg cgcggtaaac cccgacggct agccgggctt
      241 cgccttggtt cgcgccggtc ggcgggagaa gtccaccgcc atgtcgaagg cctgctttgt
      301 gaaggcgagt acctgcgcaa aggtctcatc gtcgagcgca cgcgccgcat cgtttccacg
      361 gtagacattg gctagctcgc gctcgcgtgt gccctgcacc gcgaggcgcc cgatgccgat
      421 cgactcaagc gccttccaca actcggggtt gtactggcgg gtcaatccca tcgcaaccgg
      481 gatcgggtcg cgccggccga gcacgcctgc gagtcgcagc acgatttcga agggcagcgc
      541 agccgtgccg ccctcggcct gctcgatcag ctttgtgtcg cccaggccga cagcatcgct
      601 gagttccttg gccgtcagcc cggcttcctc gcgccactgg cgcagttgcg atccggcctt
      661 gcccaccgca gccttcttct ccgggtcgcg gatgcgcgcc tgcgcgagtg tcatgccgat
      721 gtcgcccgcg ctgccggcga cgccgagcag cgtgtcggtc caactgcgca cctggcgcgc
      781 aagggacagc agcggcagtt cgcccacggc ctgcggagcc gtgcgcttgc tcttcttcga
      841 tgtggtgacc atgtcagctg cttccttctt gcgtttcact gccgcctctc ggcggctccg
      901 actcgccgcc gaacgacgcg agccacagcc cgcgcagcgc cacccagtgc gccttgtgcg
      961 cgtgatcgat cagccccaga tgatcggcaa ctgcctcgct ggccaggctg cgcaggctgg
     1021 gtcgcggcaa gcgtacgaaa gcttccgctt cgccgctgga gaccgccacc tgcaggccct
     1081 gcttgcgtgc caggtggagc atcggcgcgt tctcgaccag gcagttcatg aacagcacgc
     1141 gcacgccggc attgcgggca tgggtggcgc tgcgctcgag cagcgccgca ccgatgccct
     1201 gaccccgcgc cggcgcaagg accgacacgc cgagttcggc atgctcgccg tcgcgcgcga
     1261 ggtgtgcggc gccgatgata ttcagctcgg catcggtcgc gacaaagacg gtgtcgcgcg
     1321 caaagtcgat gccttcgaca taccgctgca ggccttcgtc tgcaatcgca tgcgcgaaac
     1381 gcagccgcct gtcctccgcc ggcatggcga gcagatgggc gagcaggctg gcgcgctcgg
     1441 tcgggttcag ttcgcgggtg accgtactca tgatcggctg cctattggtt cgcgacggcc
     1501 gcgagcgcga gcgccgcgac ctggtcgaca ctgcccagcc cgtggacgtg ccggcaacgc
     1561 ggcgcattcc tgtcgcccgt ggcgtcccac gccgcgtcgt acgcaagcag cggttgcgtc
     1621 tgagttcggt agaccaccag acgcttgcgc agggtcccct cggtgtcgtc gccacgttga
     1681 acgagctcct cgcccgtgac ctcgtccttg gtcgcgatca gtggtggggg gaaaagcgac
     1741 gtcgccgatg cggcaaggtc ccgagtgccc ttccccgcgc ccggtggccc caacaggatg
     1801 agtctcatcg cagcgcctcc gcgacgccct tgatcttcgc ggcgcgcgcg atggcccgct
     1861 ggatcagttc cttggccaag tcggcatcgc cccagccctc gaaccgggcc cacttgcccg
     1921 gctcgaggtc cttgtagtgc gcgaagaagt gttcgatctg gcgccacgcg atctcgggca
     1981 ggtcggtgta gttgtgaacg tgggcgtaac gctgcgtcag ccgtgtggcc ggtaccgcca
     2041 cgatcttctc gtcgccgccg gcttcgtccg tcatcctgag cacgcccacg gggcgcacgg
     2101 cgatggccga gcccggcatc agcgggcgcg tgttggccac cagcacgtcg cagggatcgc
     2161 cgtcctcgga cagggtgtgt ggaatgaagc cgtagttgcc cgggtagcgc atcggcgtgt
     2221 agaggaatcg gtcgacgacg agcatgcccg cacccttgtg catctcgtac ttgatgggct
     2281 cgccgccgat cggcacctcg atgacggcat tcacttcgtg cgggggatcg ctgccgatcg
     2341 gaaggttctc cagattcatc tcacgcccct ctcatgccct ggatcgcccg cgagtctcga
     2401 tgcgcgagga gttgccgcgc agaaaccatt gacgagcaga ctcgcaggtc gtacctccga
     2461 tccggcaatt gcgcgtacac ggcgacgaat cgcaggaccg cgggatgcag ggcaatgaag
     2521 acaacgaagg tgtaggggtt ggtcagcgcg ggcccggtga ggaaagcgtt gatctgcact
     2581 gcgtcggacg cgatcaatgc cacccgttcg acaggcagcg tatcgacgat catgaacctc
     2641 aggtcgtcga atctcgggtc cgcggccatc ctctggtgcg actccagaac ctcttccagc
     2701 acgaggtcgc cgcggtaggt ggtgacgatc cccgttggct cccaacagtg gttgaagggc
     2761 atcttgacaa ttcgactcgc cgttcgagag ggactctgtt gaagcgcgca tttcggccac
     2821 tttcaagcag gcgacagcgg tgccgggaat ccgaccattg gccggcaccg caccctcgaa
     2881 agcaggctca ccgctccttg tacggccgca ccgcaaacat cacgatcagg ccggccagga
     2941 tgaacatgac cagtaccatg tacatggtgc tgatcggatt gccggtgctc ttggtcagcg
     3001 cgccggccac tgcgggactc gccgccgagc cgaggttccc caagctgctg atcaaagcga
     3061 tgccacccgc cgccgcggcc ttggacaggt actcggaggt gatcgtgaag aacatcggcg
     3121 tcatggtcga gatgccgatg ctggcgatcg acatgccgat cagcgagccg accagattgc
     3181 cc
//
LOCUS       DAOORV010000295         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_36460_length_3177_cov_8.086803, whole genome shotgun sequence.
ACCESSION   DAOORV010000295 DAOORV010000000
VERSION     DAOORV010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36460_length_3177_cov_8.086803"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="hemN"
                     /locus_tag="PKV56_19415"
     CDS             complement(<1..931)
                     /gene="hemN"
                     /locus_tag="PKV56_19415"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOX70015.1"
                     /translation="MLTTTHTERPVVTEELLRRLDVNGPRYTSYPTADRFVDAFGPDD
                     YARALSQRAEGAGVIGGASPLSVYVHIPFCESLCYYCGCNKVITKHHERAGEYLDALE
                     KEIALHVDRLGAGQTVSQLHFGGGSPTFLSDAELSRLMGTLRQAFRLAPEAENSIEVD
                     PRTVNAERLAHLRQIGFNRLSFGVQDFDPAVQQAVHRIQSRESVASLMESARALGFES
                     TNVDLIYGLPKQTPESLRRTIEQVAELRPERIALYSYAHLPQRFKPQRRIDSAALPPA
                     ADKVTMLSNSIAGFVGHGYQYIGMDHFALPGDSL"
     gene            1044..1847
                     /gene="fnr"
                     /locus_tag="PKV56_19420"
     CDS             1044..1847
                     /gene="fnr"
                     /locus_tag="PKV56_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOX70016.1"
                     /translation="MTASHAHDQPAIGHDSESAPRSALPHVVPIRADAFKVACSNCNL
                     RELCLPVGLSDPDLERLDELVAKRRSVTRGDALFRTGDSFQSLYAVRTGFFKTCVSSE
                     DGRDQVTGFQMAGELLGLDGISHDRHNCDAIALEDSQVCVIPFGQLEELSRDFTPLQH
                     QFHKIMSREIVRDHGMMLLLGSMRAEERLAAFLLNLTQRLQARGFSASSLILRMTREE
                     IGSYLGLKLETVSRTFSKFQDDGLLEVKQRHIRILDEAGLRQLISSTAC"
     gene            complement(1860..2120)
                     /locus_tag="PKV56_19425"
     CDS             complement(1860..2120)
                     /locus_tag="PKV56_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70017.1"
                     /translation="MKQRALTILWPAFLMAGVLEMLVFVVVDPGDLHWFGGEALNWSN
                     QAVYTVTFLIFWAVIATAGALTALLEASPEEINADRGRSWPR"
     gene            complement(2143..2391)
                     /locus_tag="PKV56_19430"
     CDS             complement(2143..2391)
                     /locus_tag="PKV56_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70018.1"
                     /translation="MQDKPIPWYKFPMVWLVLGGPLSVVIASLATAVVAWKHIDPVIT
                     TTPQGELRAADDVAAKVSPKDALAPAMQGRNHAASPQR"
     gene            complement(2410..>3177)
                     /locus_tag="PKV56_19435"
     CDS             complement(2410..>3177)
                     /locus_tag="PKV56_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HOX70019.1"
                     /translation="FQSAMFDHDTMIISYDTARGEPRGSRPRSAPHASLAQLGKGECI
                     DCSLCVQVCPTGIDIRKGLQYECIGCAACIDVCDSVMDKMSYPRGLIRYATQNGINKG
                     WDKAQMLRRVLRPRVLVYTAILIIILAAFGASLMLRTPFRVDVVRDRGALARLVEDGR
                     IENVYRLQVMNGTEAPQRFRIEVDGLKGAIIAGKSSVEVGSTESRWVPVSVQVPPETA
                     GALGAGAHPMRFNITRLPDDSGGEITVHEKSTFVVPR"
BASE COUNT          533 a         1063 c         1038 g          543 t
ORIGIN      
        1 ccagcgagtc gccgggcagc gcgaaatggt ccatgccgat gtactggtag ccgtgaccca
       61 cgaagcccgc gatggaattg gacagcatcg tcaccttgtc ggccgccggc ggcagcgccg
      121 ccgagtcgat acggcgctgc ggcttgaagc gttgcggcaa gtgcgcgtac gagtagagcg
      181 cgatgcgttc ggggcgcagt tccgccacct gttcgatcgt gcgccgaagc gattcgggcg
      241 tctgcttcgg aaggccgtag atgaggtcga cgttggtgga ctcgaagccg agcgcgcgcg
      301 ccgattccat cagcgaggcg acgctctcgc ggctctggat gcggtgcacg gcctgctgaa
      361 cagccgggtc gaagtcctgc acgccgaagc tcaggcggtt gaagccgatc tgccgcaggt
      421 gagcgagccg ttcggcgttc acggtgcgcg gatcgacctc gattgagttc tcggcttccg
      481 gtgcgaggcg gaacgcctgg cgcagcgtac ccatcaggcg gctgagctcc gcatcgctca
      541 ggaaggtggg cgagccgccg ccgaagtgca gttgcgagac ggtctggccg gcgccgagcc
      601 ggtcgacgtg cagcgcgatc tccttctcga gcgcgtcgag gtactcgccg gcgcgttcgt
      661 gatgcttggt gatgaccttg ttgcagccgc agtagtagca caacgactcg cagaagggga
      721 tgtgcacgta gaccgacagc gggctcgcgc cgccgatcac gcccgcgcct tcggcacgtt
      781 ggctcagtgc gcgcgcatag tcatcaggcc cgaaagcgtc gacgaaacgg tcggctgtcg
      841 ggtacgaggt gtagcgcggc ccattcacgt cgagccgtcg cagcagctct tcggtcacga
      901 cagggcgctc ggtatgtgtg gtggtcagca tggagcaaga ctgtgccgcg acgcgcgccc
      961 ggcgacttga cgtgcatcaa cagcgcttca gttggcgaac cggacaataa ttccatgcgc
     1021 accaccactc cgaatcgccg tacatgaccg cgtcgcatgc tcacgatcag cccgccatcg
     1081 gccacgattc cgagtccgct ccgcggtcgg ccctgcccca tgtcgtgccg atccgcgccg
     1141 atgcattcaa ggtggcatgc tcgaactgca acttgcgtga gctgtgcctg ccggtgggct
     1201 tgtccgaccc cgatctcgag cggctcgatg aactggtggc caaacgccgc tcggtgaccc
     1261 gcggcgacgc cttgttccgc accggcgact cgttccagtc gctctatgcg gtgcgtaccg
     1321 gattcttcaa gacctgcgtc tcctcggaag atggccgtga ccaggtcacc ggtttccaga
     1381 tggccggcga gttgctcggc ctcgacggca tcagccacga ccgccacaac tgcgacgcga
     1441 tcgccctgga agactcgcaa gtctgcgtga tcccgttcgg tcagctcgag gaactgtcgc
     1501 gcgacttcac gccgctgcag caccagttcc acaagatcat gagccgcgag atcgttcgcg
     1561 accacggcat gatgctgctg ctgggcagca tgcgcgccga agagcgcctt gccgccttcc
     1621 tgctcaacct gacgcagcgg ctgcaggcac gcggcttctc cgcctcatcg ctcattctgc
     1681 gcatgacgcg cgaagagatc ggcagctacc tcggcctcaa gctggaaacc gtgagccgca
     1741 ccttctcgaa gttccaggac gatggcctcc tggaggtcaa gcagcgccac atccgcatcc
     1801 tcgacgaggc cgggctgcgc cagctgatca gcagcaccgc ctgctgaaga tctgcccgct
     1861 cagcgcggcc agctgcggcc gcggtcggcg ttgatctctt ccgggctcgc ctccagcagc
     1921 gccgtcagtg ccccggcggt cgcgatgact gcccagaaga tcaggaaggt gacggtatag
     1981 accgcctggt tggaccagtt cagcgcctcg ccaccgaacc agtgcagatc gcccggatcg
     2041 acgaccacga agacaagcat ctccagcacg ccggccatca ggaaggcggg ccagagaatg
     2101 gtcagcgcgc gctgcttcaa gaccgccccg gggatgttca tctcagcgct gcggtgacgc
     2161 ggcgtggttg cggccttgca tggcgggcgc cagcgcatct ttcgggctga ccttcgccgc
     2221 cacgtcgtcg gcggcgcgca gttcaccctg tggcgttgtg gtgatcaccg gatcgatgtg
     2281 cttccaggcc accaccgcgg tggccaggct ggcgatgacg accgacagcg ggccgccgag
     2341 caccagccac accatcggaa acttgtacca ggggatcggc ttgtcttgca tggctcagcc
     2401 cttctgtgtt cagcgcggca cgacgaaggt cgacttctcg tgcacggtaa tctcgccacc
     2461 ggagtcatcc ggcaggcgag tgatgttgaa tcgcatcggg tgcgcaccgg ctcccagcgc
     2521 gccggccgtt tccggcggca cctggacgct caccggcacc cagcgcgatt cggtcgaacc
     2581 gacctcgacc gacgacttgc ccgcaatgat ggcgcccttc aggccgtcca cctcgatgcg
     2641 gaagcgctgc ggcgcctccg tcccgttcat cacctgcagg cggtagacgt tctcgatgcg
     2701 gccgtcttcc accaggcggg ccagcgcacc gcggtcgcgg accacgtcga cacggaacgg
     2761 cgtgcgcagc atcaggctgg ctccgaaggc cgctaggatg atgatcagga tggcggtgta
     2821 gacgagcacg cgcgggcgca gcacgcgccg cagcatctgc gccttgtccc agcccttgtt
     2881 gatgccgttc tgggtggcgt agcggatcag gccgcgcgga tagctcatct tgtccatcac
     2941 gctgtcgcac acgtcgatgc aggcggcaca gccgatgcac tcgtactgaa ggcccttgcg
     3001 gatgtcgatg ccggtcgggc agacctgcac gcacaacgaa cagtcgatgc actcgccctt
     3061 gccgagctgc gccagcgacg catgcggggc cgatcgcggc cgcgaaccac gaggctcgcc
     3121 gcgcgcggtg tcgtagctga tgatcatcgt gtcgtgatcg aacatcgcgc tctggaa
//
LOCUS       DAOORV010000296         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_36678_length_3164_cov_15.737536, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000296 DAOORV010000000
VERSION     DAOORV010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36678_length_3164_cov_15.737536"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..482
                     /locus_tag="PKV56_19440"
     CDS             252..482
                     /locus_tag="PKV56_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70020.1"
                     /translation="MNSDLGSFLRLARGLLRLYSLAVASEHRGVADHLLCALEQLAKS
                     EPAIEYLLDRAYLRGIGHHDSADPHTQRAKPQ"
     gene            complement(518..2998)
                     /locus_tag="PKV56_19445"
     CDS             complement(518..2998)
                     /locus_tag="PKV56_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX70021.1"
                     /translation="MQPLALTLKGFRGIRDGLGRDEITLDLERLTQDAQLIAIVGANG
                     SGKSTIMDCLVPYIGLPSRAALAGAGGFSYYDHVYLPESVKDLTWAHEGRCYRSQIVI
                     RLCGRRRTEAFLHTLDDAGHWRPVQLDDGMVSDGRVETYTRCVEAICGSAETFFTSVF
                     AAQGKRQLSTYRNAEIKSLLADLLGQEEIQALGQKAAETVRLLKAGLATIRQELVGLD
                     AEGVRLEATRRRLDGAGERMAAAERAKTAAHSALEAAQARHARVAVEREQSRATEARR
                     AQLAAERQSTLDTTAQARRALQSRDQVERQRLEQLDQRVAGRRQQECSRRQALTLSRQ
                     RCQAVLAEAGEVQRADRRLPLAQRVQALRSALTEACRQQTHALTRCQGAVRLAEHRVA
                     GIEQEAGRAALKAEELARRFGLTGEVPCVGTDLQGRCKLLGDAREAQSLIPNAHAQLA
                     RLAVEKAAAQQELLAARQRCEALAGAPQNLARAERREAVARERVSRLAVMCSKAPACA
                     QAQMTLVEIDQELQALGPALAADAAAETADERAERQQIAAARQAIEEQQEQLARQATA
                     ALARLDHALASLPPAFDERQVTQAAQALGAAREAFGATEQSMLTAVREAQTIEEVSKQ
                     VAALAERRERVHARSARVEDELGNWSLFARCMSNDGLIALAIDDAGPALSALANDLLL
                     ACYGPRFTVSIHTLLETAKGEQKEGFDILVHDGDTGESKSVSLMSGGERTFVESCLTR
                     AIALYLARNTGRRYTTLFTDEADGALDSQRKRMFMAMKREVLRLGGYEREFFVSQTPE
                     LTAMADAVIDLDALKADRCEAQRSVSAA"
BASE COUNT          431 a         1135 c         1071 g          527 t
ORIGIN      
        1 ccaagctcgc ggcactggtc gaccactact tcgcagcctg gcaggagacg cggctgcaac
       61 tcgtgcggcg cgaccgggag ttggccgaag tgcgccgcgc ccacaagccc caggtggttt
      121 cgctgcagcg ctgaacctcc gccgcagtct cgctccgagg gagggagtgc ggccgggggt
      181 tgactttgac acaggggcaa gctctacgct gcgagcgctc ggggcagtgg tgccccgctt
      241 ggaggtctgc gatgaacagc gacctcggat ccttccttcg cctcgcgcga gggctgttgc
      301 ggctctacag cctggccgtc gcgagtgagc accgcggcgt tgcggatcac ctgctgtgcg
      361 ccctggagca gctcgcgaag agcgagccag cgattgagta cctgctcgat cgggcatacc
      421 tgcgtggaat cggtcaccat gattcggccg atccgcacac gcagcgagcc aagccgcaat
      481 gaccgatgcc ggcgctgccg gcttgggccc gcggccgcta cgccgcggag acggaccttt
      541 gcgcttcgca tcggtccgcc ttcagcgcgt cgaggtcgat cacggcgtcg gccatcgccg
      601 tcagctccgg cgtctgcgag acgaagaact cgcgctcgta gccgcccagg cgcagcactt
      661 cccgcttcat cgccatgaac atgcgcttgc gctgggagtc cagcgcgccg tccgcctcgt
      721 cggtgaacag cgtcgtgtag cggcggccgg tgtttcgcgc caggtacaac gcgatcgcgc
      781 gggtcaggca tgactccacg aacgttcgct cgcccccgct catcaggctg acgctcttgc
      841 tttcgccggt gtcgccgtcg tgcacgagga tgtcgaagcc ctccttctgt tcgcccttgg
      901 cggtctccag caaggtgtgg atcgacaccg tgaagcgcgg tccgtagcag gccagcagca
      961 ggtcgttcgc caacgccgag agcgcgggcc ccgcatcgtc gatcgccagc gcgatcaggc
     1021 cgtcgttgct catgcagcgc gcgaagaggc tccagttgcc cagttcgtcc tcgacgcgag
     1081 cgctgcgcgc gtgaacgcgc tcgcggcgct cggccagcgc ggccacttgt ttggacacct
     1141 cctcgatcgt ctgcgcttcg cgcaccgccg tcagcatcga ttgctcggtc gcgccgaagg
     1201 cttcccgagc ggcacccagc gcttgagcgg cctgcgtcac ctgtcgctcg tcgaaggcgg
     1261 gcggcagcga cgcaagcgcg tgatccaggc gcgccagcgc cgccgtggcc tgcctcgcca
     1321 gttgttcctg ttgctcctcg atcgcctggc gtgccgcggc gatttgctgg cgctcggcgc
     1381 gctcgtcggc ggtttcggca gccgcatcgg cggccagcgc agggcccagc gcctgcaact
     1441 cctggtcgat ctcgaccaat gtcatctgtg cctgtgcgca cgccggcgcc ttcgagcaca
     1501 tcacggccag gcgactcacc cgctcgcgcg cgacggcttc gcggcgctcg gcccgtgcca
     1561 ggttctgcgg tgcgccggcc agcgcttcgc agcgctggcg cgcggcgagc agttcctgct
     1621 gcgccgcggc cttctcgacg gccagccggg ccaactgggc atgggcgttc gggatcagcg
     1681 attgcgcctc gcgcgcgtca cccagcagct tgcaacggcc ctgcaggtcc gtgccgacgc
     1741 agggcacctc gccggtcagg ccgaaccggc gcgcgagttc ctcggctttc agcgcggccc
     1801 gccccgcctc ttgctcgatg cctgccacgc ggtgttcggc aagccgcacc gccccttggc
     1861 agcgcgtcag cgcgtgtgtc tgctggcgac aggcttcggt gagcgcgctg cgcagcgcct
     1921 gcacacgctg cgcgagcggc aggcgccggt ccgcacgctg tacctcgccg gcctcggcaa
     1981 gcactgcctg gcaacgctgc cgggacaggg tcagcgcctg gcgccgcgag cattcctgct
     2041 gacgccgtcc ggcgacccgc tggtcaagtt gctccagccg ttgccgctcg acctgatccc
     2101 tggactggag cgcacggcga gcctgcgcgg tggtgtcgag agtggactgg cgttcggccg
     2161 cgagctgtgc gcggcgagct tcggtggccc gcgactgctc gcgctcgacc gccacgcgcg
     2221 catgccgcgc ctgcgctgcc tccaatgcgg aatgcgccgc cgtcttggca cgctcggccg
     2281 ccgccatgcg ttcgcccgct ccgtcgagcc gccggcgtgt cgcctccagg cgcaccccct
     2341 ccgcgtcgag cccgaccagt tcctgccgga tggtcgccaa ccccgccttc aacaggcgca
     2401 ccgtctctgc ggccttctgg cccagggcct ggatctcttc ctgcccgagc aggtccgcca
     2461 gcagcgactt gatctcggca ttgcgatagg tgctgagctg gcgcttgccc tgcgccgcga
     2521 acacggaggt gaagaacgtc tcggcgctgc cgcagatggc ctcgacgcag cgggtatagg
     2581 tctcgacccg gccatcggac accatgccgt cgtcgagctg caccggccgc cagtgcccgg
     2641 catcgtcgag cgtgtgcagg aaggcctcgg tgcggcggcg gccacagagg cggatgacga
     2701 tctgcgagcg gtagcagcgg ccctcatgcg cccaggtcag gtctttcacg ctttcgggca
     2761 ggtacacgtg gtcgtagtag gagaagccgc ccgcaccggc cagcgcggcg cggctcggca
     2821 gtccgatgta gggcacgagg cagtccatga tcgtcgactt gccgctgccg ttcgccccga
     2881 cgatggcgat cagttgcgcg tcctgggtca ggcgctcgag gtccagcgtg atctcgtcgc
     2941 ggcccaggcc gtcacgaatg ccgcggaatc ccttgagggt gagtgcgaga ggttgcatgg
     3001 agagccccga ggaagcagat gcctcaggct ctcatgagca gtctctacgt caaggccaat
     3061 caactgcgcc catggcacgc gtctcagccg gcacagcacg agagctgcgt gacgtccttg
     3121 gtgaaggtct gcgcgcacag cttgagccct tccaccatgg tcag
//
LOCUS       DAOORV010000297         3145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_37000_length_3145_cov_12.340777, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000297 DAOORV010000000
VERSION     DAOORV010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37000_length_3145_cov_12.340777"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKV56_19450"
     CDS             <1..321
                     /locus_tag="PKV56_19450"
                     /inference="COORDINATES: protein motif:HMM:NF019154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOX70022.1"
                     /translation="ALVHVVAEGLLLASPRIFREFAKQQVGVPESAADAAKRVQREVL
                     REGWHLRADRGVNILCYERKRSDRGSSRINGIVICEPQRFIQALPAIDSTLVRVVEGA
                     SVPT"
     gene            321..2333
                     /gene="traD"
                     /locus_tag="PKV56_19455"
     CDS             321..2333
                     /gene="traD"
                     /locus_tag="PKV56_19455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HOX70023.1"
                     /translation="MAYPLEALLRPAHELRSACASALGAGMVIGAPGLFLLSRPWDWL
                     LAGALVAHAAWRGAAGLQTLRYRANLRRQRRYEVTSTEIPWSTERLFLGRGFQWDQRH
                     TQRLFEARRPESQPLLQQGLLVELFARLRPQVAAPVGLGGDPAIHGVEPGEADIWMDI
                     ADRVGHTLVLGTTRVGKTRLAELLITQDIRRGEVVIVFDPKGDIDLLRRIYAEAERAG
                     RADEFFMFHLGHPEISARYNPVGSFSRITEVATRIAGQLPSEGQSAAFKEFVWRFVNV
                     MARALVALGRKPDYQEINRYASDVEPLLVDYFEHWFDQEPAAAGWREALQAMAIDKKS
                     LDKGLQSRGARAVSLVEYARRRKLYDPIAHALASTLNYEKSHFDKLVASLLPLMEKLT
                     TGRTAALLSPTLDDPTDWRPVFDWTSVINLGGIVYVGLDALSDYEVAGAVGNSMFADL
                     TSVAGSLYKFGPGRGLPGEIRPRRIAIHADEFNELIGDEFIPLLNKAGGAGFQVTAYT
                     QTWSDVEARIGSPAKAGQIAGNFNTLIMLRVKEVATAELLTDQLPQVQVVSTIVSSSV
                     SDTNDPGEFTDFASRNEDRIAPESVRMLEPTDLVQLPKGQAFALIHGGQLHKIRMPLP
                     SAEHDPLMPEGLTVIGQALRSRFDGPWNRPQEYEDRATEVEARRGE"
     gene            2323..3036
                     /locus_tag="PKV56_19460"
     CDS             2323..3036
                     /locus_tag="PKV56_19460"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HOX70024.1"
                     /translation="MASNNTSFHDGALAGVLLSPLKLAFSVGLGLVAILLVAWSIDWV
                     CVSRVWPDGVDRLHSLLAADLGHGIALAARQGRGSGEITGPANLLYGLVFEVTGIHDM
                     GLRFAEPSALSIPDTVVRRSYLANREAIETAMVGTQLLGVRFATFMRFLPLLLLLYAV
                     GAADGLTERAIRRSCGGRESASLYHRAKYLQMVALGLGGVALLVWPGPVAWELCAGLI
                     VILTAWLARQQWAFYKKHL"
BASE COUNT          499 a          967 c         1105 g          574 t
ORIGIN      
        1 gcgctcgtgc atgtggtggc tgaaggcttg ttgcttgcct cgccacgcat cttccgggag
       61 ttcgccaaac agcaagtcgg ggtgccagag tccgctgccg atgcggccaa gcgcgtgcaa
      121 cgcgaggtct tgcgcgaggg ctggcacctg cgtgccgatc gcggcgtgaa catcctttgc
      181 tacgagcgca agcggtccga ccgtggcagt tcgcggatca atggcatcgt gatctgcgag
      241 ccgcagcgat tcatccaggc actgcccgcc atcgactcga cgctggtgcg cgtggtggaa
      301 ggcgcgagcg tgccgacctg atggcttacc cgctagaagc cctgctgcgg ccggcgcatg
      361 aactgcgttc ggcctgtgcg tcggcgctcg gcgcgggcat ggtgatcggt gcaccgggcc
      421 tgttcctgct gagccgaccg tgggactggc tgctggccgg tgcattggtg gcgcacgcgg
      481 cctggcgagg cgctgccggc ctgcaaaccc tgcgctaccg tgccaacctg agacgtcagc
      541 gccgttacga ggtgacctcg accgagatcc cgtggtcgac cgagcggctg tttctggggc
      601 ggggtttcca gtgggaccag cgccataccc agcgcctgtt cgaggcccgt cggcccgaga
      661 gccagccgct gttgcagcaa ggtttgctcg ttgaactgtt cgcgcgcctg cgtccgcagg
      721 tggccgcacc cgtcggcctg ggcggcgatc cggcgatcca cggggtcgag ccgggtgaag
      781 ccgacatctg gatggacatc gccgaccggg ttggtcacac gctggtgctg ggtaccacgc
      841 gggtgggcaa gacgcgcctg gcggaactgc tgatcaccca ggacatccgt cgcggggagg
      901 tggtgatcgt gttcgacccg aagggtgata tcgacctgct gcgtcgcatc tatgccgaag
      961 ccgagcgggc cgggcgcgcg gacgagttct tcatgttcca cctcggacac ccggagatct
     1021 cggcgcgcta caacccggtg ggcagcttct cgcgcatcac cgaggtggcc acgcgcatcg
     1081 ccggccagct gccgtcggaa gggcagtcgg ccgcgttcaa ggagttcgtc tggcgcttcg
     1141 tcaacgtgat ggcgcgcgcg ctggtggcct tgggccgcaa gcctgattac caggagatca
     1201 accgctacgc atccgatgtc gagcccttgc tggtcgacta cttcgagcac tggttcgacc
     1261 aagagccggc tgccgccggt tggcgtgagg cattgcaggc catggcgatc gacaagaaga
     1321 gtctggacaa gggactgcag tcgcgcggcg cacgagcggt cagcctggtc gagtacgcgc
     1381 gccgccgcaa gctctacgac ccgatcgccc atgcgttggc ctcgaccctg aactacgaga
     1441 agagccactt cgacaagctg gtggcgtcgt tgctaccctt gatggaaaag ctcaccaccg
     1501 gccgcacggc agcgctgctg tcgccgacgc tcgatgatcc gaccgactgg cggccggtgt
     1561 tcgactggac ctcggtgatc aatctgggcg ggatcgtcta tgtcgggctc gatgcgctgt
     1621 ccgactacga ggtggcgggc gcggtgggca attccatgtt cgcggatctc accagcgtgg
     1681 ctggcagcct ctacaagttc ggtccagggc gcggactgcc gggcgagatc cgaccgcggc
     1741 gcatcgccat ccatgccgac gagttcaacg agctgatcgg cgacgagttc atcccgctgc
     1801 tgaacaaagc cggtggggcg ggttttcagg tgacggcgta cacgcagacc tggtcggacg
     1861 tggaagcgcg catcggctca cccgccaaag ccggccagat cgccggcaac ttcaacacgc
     1921 tgatcatgtt gcgggtgaag gaggtggcca cggccgagtt gctgaccgac cagttgccac
     1981 aggtccaagt ggtgtcgacc atcgtgtcgt cgtcggtttc ggacaccaac gatccgggcg
     2041 agttcaccga cttcgccagc cgcaacgagg accgcatcgc cccggagagc gtgcgcatgc
     2101 tggagccgac cgatctggtg cagttgccca agggccaggc ctttgcattg atccatgggg
     2161 gccagttgca caagatccgg atgccgctgc ccagtgccga acacgacccg ctgatgcccg
     2221 agggcctgac ggtgatcggg caggcactgc gaagccgatt tgacggccca tggaatcggc
     2281 ctcaggaata cgaggatcgg gctacggaag tggaggcccg ccgtggcgag taacaacacg
     2341 tcgtttcacg atggcgcctt ggccggtgta ctgctgagcc cgctgaagct ggcgttctcg
     2401 gtggggctgg gattggtggc gatcctgctg gtcgcatgga gcatcgactg ggtgtgcgtg
     2461 tcgcgggtct ggcccgatgg tgtggaccgg ttgcacagcc tgctggcagc cgatcttggg
     2521 cacggcatcg cgctggcggc acggcagggc cgggggtctg gcgagatcac cgggccggcg
     2581 aacctgctgt atggcttggt gttcgaggtc accggcatcc atgacatggg gctgcgcttc
     2641 gctgaaccat cggccttgtc gatcccggat acggtggtgc gtcgcagcta cctggccaat
     2701 cgcgaggcca tcgagaccgc gatggtcgga acgcagttgc tgggcgtgcg attcgcaacg
     2761 ttcatgcgat tcctcccgct gctgctgctc ctgtacgcgg tgggcgcagc cgacggactg
     2821 accgagcgcg cgattcgcag gtcctgcggc gggcgagagt cagccagcct gtaccaccgg
     2881 gcgaagtacc tgcagatggt ggcactgggg ctgggcgggg tcgcgctgtt ggtctggccg
     2941 gggcctgtgg cctgggagct ttgcgctggg ttgatcgtca tcctcactgc ctggctggcg
     3001 cgccagcaat gggcgttcta caagaagcac ctgtaggtcg accggggtcg gtgctacagg
     3061 tacttccggt aggtatggct gttcttgagg tcgatgccca tgacttgcat gatccgcgca
     3121 cgagcctctt catcgtcgac gtaaa
//
LOCUS       DAOORV010000298         3122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_37349_length_3122_cov_14.636779, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000298 DAOORV010000000
VERSION     DAOORV010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37349_length_3122_cov_14.636779"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..440
                     /locus_tag="PKV56_19465"
     CDS             12..440
                     /locus_tag="PKV56_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX70025.1"
                     /translation="MSAKDIEMARVAQRCIMEALDHSRAAAITLTTDSGEHPTVDLPP
                     AALKLIGQLLGVMSEGRPITLVPSSQELSTVEAAHFLNVSRPFVVKEIDAGRLAHRLV
                     GTHRRVAFEDLIAYARKMREQQESALERMAENAREMGLDY"
     gene            442..1023
                     /locus_tag="PKV56_19470"
     CDS             442..1023
                     /locus_tag="PKV56_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX70026.1"
                     /translation="MAGHARYTALLDACVLYPLAMADALMSLATTGLFAAKWTTRIEA
                     EWIRSLEENRPDLLGRLLYRRDQMRDAVPDWEVDERAWTVCARGLVLPDPDDVHVLGA
                     ALAGHADCIVTANLKDFPADVVVPLGIEVIHPDQFIVAQWGLDQLVAVAAFKRMRARW
                     KKPEASAEDFAAAMERGGLPATAQRLREAAELI"
     gene            1226..>3122
                     /locus_tag="PKV56_19475"
     CDS             1226..>3122
                     /locus_tag="PKV56_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HOX70027.1"
                     /translation="MSESGPEVDGSCPDKRLRVEATLIAKLNLADFQNAVPMLRELRI
                     VNDTTLAAQDLELRIASEPGFLKPKTWRIDAVAAGDSCRIPDLDVQLDGALLTRLTEA
                     EQAVVTLTLHQRGGDAPDRARFERTVELLPRNQWGGLSHLPDLVAAFVQPNEPAVERL
                     LKQAAEVLRKNGRSAALDGYKGGSKRAWELSSAIWSAVAAMGLDYALPPASFEHTGQK
                     VRGPGQIAESGLATCLDLALLFCAAIEQAGLNPLLVFTKGHAFAGVWLRAEEFSSTVV
                     DDVTALRKRVKLKELLLFETTLATQRPAPTFSFAAQHAAQQIAEDKEEAFELAVDIRR
                     ARMQRIKPLASAEAPAVAAPPEPAAVVEPAFDAPPDLPDDNASADADLATLNPKDRLA
                     RWQRKLLDLSLRNNLLNFKTGKKARKLEAPDAGALEDLLSDGQALKLLPRPDLMDGAD
                     PRNQAIHEGREREDLRRAHALDALKRRELFIALPQDDLEPRLVELFRTARTTLQEGGA
                     NTLFLALGFLTWTRDDKAGQKYRAPLVLVPVTLHRWSARSGFTLALHDDEPRFNPTLI
                     EMLRQDFKLDLGVADGELPRDEAGLDVRAVWNTVSRAIKDIKGWEVSEEVVLAMFSFA
                     KYLMWKDL"
BASE COUNT          550 a         1017 c         1064 g          491 t
ORIGIN      
        1 caccgcccgc catgtccgcg aaggacatcg agatggctcg cgtggctcag cgctgcatca
       61 tggaagcgct ggaccactcg cgcgccgcgg ccatcacgct gaccaccgac agcggtgaac
      121 accccaccgt cgacctgccg cccgcagcgt tgaagctgat tggccaattg cttggcgtca
      181 tgagcgaggg acgaccgatc acgttggtgc catcgagcca ggaactcagc accgtcgagg
      241 cggcccactt cctcaacgtg tcccggccgt tcgtcgtcaa ggagatcgat gcgggccgac
      301 tggcgcatcg actggtcggt acgcaccggc gagtcgcctt cgaggacctg atcgcctacg
      361 cccgcaagat gcgcgagcag caggagtccg cgctcgaacg catggccgag aacgcgcgcg
      421 aaatggggct ggactactga cgtggctggc catgcccgct acacggcact gttggatgcg
      481 tgcgtgctgt atccgctggc catggccgac gcgctgatga gcctggccac cacggggctg
      541 ttcgcagcca aatggacgac ccgcatcgaa gccgagtgga tccggtcact ggaggaaaac
      601 aggcccgacc ttctagggcg gctgctgtac cgccgcgacc agatgcggga tgcggtgccg
      661 gactgggagg tggacgaacg cgcctggacc gtctgcgcgc gcgggttggt gttgcccgac
      721 cccgatgatg ttcacgtgct aggagccgcg ttggcggggc acgccgactg catcgtcaca
      781 gccaatctga aggactttcc cgcagacgtc gtggtgccct tggggatcga ggtgatccac
      841 cctgaccagt tcatcgtggc gcagtggggt ctggaccagt tggtggccgt ggcggccttc
      901 aagcgcatgc gggcacggtg gaagaagcct gaagccagcg cggaggactt tgccgccgcg
      961 atggagcgag ggggactgcc cgccacggca caacgcctgc gtgaggctgc tgagctcatc
     1021 tagttgtggc ccgtgctcgt cggttgccga cggcagggtg gacgactatt tcgctgaatg
     1081 gcgaacgcga gagtcctgtc cggcaggtgt gacaagatgc gtcggatgag gggtcttgat
     1141 ttgccgcaat ggcgcatcga cagaccacca aggggagggc cctcgacggc ccgcagtgca
     1201 agctcggtct gcagggtggc gcgccatgtc ggaatcaggt cctgaagtcg atgggtcatg
     1261 ccccgacaag cgtctgcgcg ttgaagccac gctgatcgcc aagctcaacc ttgcggattt
     1321 ccagaacgcg gtgccgatgc tcagggaact gcgcatcgtc aacgacacga ctctggccgc
     1381 ccaggacctt gaactgcgca ttgcctcgga gcccggcttc ctcaagccga agacctggcg
     1441 gatcgatgcg gtggcggccg gcgacagctg ccgaatcccc gatctggatg tccaactcga
     1501 cggcgcgctg ctgacacggc tgaccgaggc ggagcaagcc gtggtcaccc tgaccttgca
     1561 ccagcgtggt ggcgatgcgc ccgaccgggc ccggttcgag cgcacggtcg agctgctgcc
     1621 gcgcaaccag tggggtggct tgtcccatct gccggacctg gtggcggcct ttgtgcagcc
     1681 caacgagccc gccgtggagc ggctgctcaa gcaggccgcc gaggttctcc gcaagaacgg
     1741 caggagcgcg gcgctggacg gctacaaagg tggcagcaaa cgcgcctggg agctgagttc
     1801 ggccatctgg agcgcggtcg ccgcgatggg cctggactac gcgctgccgc ccgccagctt
     1861 cgagcacacc ggtcagaagg tgcgcgggcc gggacagatc gccgagtccg gcctggccac
     1921 ctgtctggac ctggctctgc tgttctgtgc ggccatcgaa caggctgggc tgaatccctt
     1981 gctggtgttc accaaaggcc acgccttcgc cggcgtatgg ttgcgcgccg aagagttctc
     2041 gtccactgtc gtcgatgacg tcacggcgct gcgcaaacgc gtcaagctca aggaactgct
     2101 cctgttcgag accacgctgg ccacccagcg gccggcaccg acgttcagct ttgccgcgca
     2161 gcatgcggcg cagcagattg ccgaagacaa ggaagaggcc ttcgagctgg cggtcgacat
     2221 ccgccgggcg cgcatgcagc gcatcaagcc gctggccagc gccgaagcgc ctgcggtcgc
     2281 cgcgccaccg gagccggcgg ccgtcgtgga accggcattc gacgcgccgc cggacctgcc
     2341 ggacgacaac gccagcgccg atgccgacct ggccacgctc aacccgaaag acaggctggc
     2401 acgctggcag cgcaagctgc tggatctgtc gctgcgcaac aacctgctca acttcaagac
     2461 cggcaagaag gcccggaagc tggaggcgcc ggatgcgggg gcgctggagg acctgctgtc
     2521 cgacggccag gccctgaaac tgttgccgcg tccggacttg atggacggcg cggacccacg
     2581 caaccaggcc atccacgagg gacgtgagcg cgaagacctg cgccgagccc atgcactcga
     2641 tgccttgaag cgccgcgagc tgttcatcgc cttgccgcag gacgacctcg aaccccggct
     2701 ggtggagctg ttccgcaccg ccaggaccac gctgcaggaa ggcggtgcca acactctgtt
     2761 cctggccttg gggttcctga cctggacccg cgatgacaag gccggccaga agtaccgcgc
     2821 gccgctggtc ctggtgccag tgaccctgca ccgctggagc gcccggtccg gcttcacgct
     2881 ggccctgcat gacgacgagc cgcgcttcaa ccccacgctc atcgagatgc tgcgccagga
     2941 cttcaagctc gacctgggcg tggccgatgg tgaactgcca cgcgacgaag cagggctgga
     3001 tgtcagggcg gtctggaaca cggtgtccag ggccatcaag gacatcaaag gctgggaggt
     3061 gtccgaagag gtggtgctgg cgatgttctc cttcgccaag tacctgatgt ggaaggacct
     3121 ca
//
LOCUS       DAOORV010000299         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_37501_length_3112_cov_7.675826, whole genome shotgun sequence.
ACCESSION   DAOORV010000299 DAOORV010000000
VERSION     DAOORV010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37501_length_3112_cov_7.675826"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKV56_19480"
     CDS             <1..362
                     /locus_tag="PKV56_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX70028.1"
                     /translation="SLGYDVDRYKLLAFVLSTALAGLAGSLKTLVLGFATLSDAHWSL
                     SGEVVLMTLLGGMGTFAGPVLGAFTIIGLQDYLSDRVGSWVTVIIGATFVACVIAFRR
                     GFVGELLAWRKRRSGLG"
     gene            642..1826
                     /locus_tag="PKV56_19485"
     CDS             642..1826
                     /locus_tag="PKV56_19485"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOX70029.1"
                     /translation="MNAREVVLCHPVRTAIGTYSGTLKDLPAPALGAVAIRECLRRSG
                     LSGDRVQSVVMGNVIQAGVRMNSSRQAAIGGGLPVEVPAMTVNRVCGSGAQAIATAAT
                     EIWAGLVDCAIAGGMENMDRAPYLLPQGRWGARMGDVSLIDSMLHDGLNDAFSSKHSG
                     WHAEDLVTRNNITREDQDRWALRSQQRFAAAQAAGLFGAEIAAIEVPGRKGPTVFDKD
                     EHNRPETTAESLAKLKPAFRKEGTITAGNSPGLNSGASAMLLAERGWAEKNGLVPMAR
                     LASFGLAAVEPDFFGLGPAPSVHQALARAGWSVADVDRVEINEAFAAIALSCLREIGF
                     AEDVVNVEGGAIAHGHPIGASGAILSTRLMYSMKRDGLRRGIVTLCIGGGQGIALAIE
                     ML"
     gene            1829..>3112
                     /locus_tag="PKV56_19490"
     CDS             1829..>3112
                     /locus_tag="PKV56_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOX70030.1"
                     /translation="MAASAGVDSATERPGFLAKERTIAVPGFNRWLVPPAALAIHLCI
                     GMAYGFSVFWLPLSKAIGIQKSMVCPADMSLLAELFTTACDWRISSLGWMYTLFFVLL
                     GSSAAIWGGWLERAGPRKAAFVAALCWCGGLVISAVGVKTHQLWLLWLGAGFIGGIGL
                     GLGYISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGSPLAAELMKHFATATDVGVAS
                     TFLVLAAIYLVFMMGGALAYRVPPSGWKPAGWTPPAAKVNDAMVTRRHVHVKKVWGIP
                     QFWLLWMVLCMNVSAGIGVIGMASPMLQEVFGGQLIGQPLHFSELDATQLGAIAGVAA
                     GFTALLSLFNIGGRFFWASLSDLLGRKSTYAVFFVLGAALYASIPASAGAGRQLLFVA
                     AFCIIVSMYGGGFAAVPAYLADLFGTQMVGAIHGRL"
BASE COUNT          405 a         1019 c         1124 g          564 t
ORIGIN      
        1 tctcgctggg ctacgacgtc gaccgctaca agctgctggc cttcgtgctg tcgacggcgc
       61 tggccggtct ggccggttcg ctgaagaccc tggtgctggg ctttgccacg ctgtccgacg
      121 cgcactggtc gctgtcgggc gaagtggtcc tgatgacgct gctgggcggc atggggacct
      181 tcgccggccc ggtgctcggt gcgttcacga tcatcggcct gcaggactac ctgtccgacc
      241 gcgtgggctc gtgggtcacg gtgatcatcg gcgcgacctt cgtcgcctgc gtcatcgcgt
      301 ttcgccgcgg cttcgtcggc gaactgctgg cgtggcgcaa gcggcgttcg gggctgggct
      361 gagccggcgt accgcctcgg ggcgatccgg gaacctgccg gtctgcgcgg cgatgaaatc
      421 ggtccggacc acgtggcgtt gatttgcgtc aacgcgggtg cgggtgatcg gcccgcccgc
      481 ccggctcacg ggccggaacc ggtctaggat tcgtcgcagc cggccggttg cgactcgcgc
      541 tggggcgtgt tccggcctgg cgccccgccg ttttattcgc ggcgtccggg tgcgggtttc
      601 accccgcccg ccgattgccg aactgcacca ggagatccat catgaatgct cgtgaagtcg
      661 ttctttgcca ccccgtgcgc acggccatcg gcacctactc cggcacattg aaggatctgc
      721 cggctccggc cctgggggcg gtcgcgatcc gcgagtgcct caggcgctcg gggctgtcgg
      781 gcgaccgggt gcagtcggtg gtgatgggca acgtgatcca ggccggcgtc aggatgaatt
      841 cgtcgcggca ggccgccatc ggtggcgggt tgcctgtcga ggtgcccgcg atgacggtca
      901 accgcgtctg cggttccggc gctcaggcga tcgcgacagc cgccaccgag atctgggccg
      961 gcctggtcga ctgcgccatc gccggtggca tggagaacat ggatcgcgcg ccctacctgc
     1021 tgccgcaggg ccgctggggt gcccgcatgg gcgatgtctc gctgatcgac agcatgctgc
     1081 acgacggact caacgacgcg ttcagcagca agcattcggg ctggcatgcc gaagacctgg
     1141 tcaccaggaa caacatcact cgcgaggacc aggaccgctg ggcgctgcgc tcgcagcagc
     1201 gctttgccgc ggcgcaggcc gccggtctgt tcggcgccga gatcgctgcg atcgaggtgc
     1261 ccggtcgcaa gggaccgacg gtgttcgaca aggacgagca caaccggccc gaaaccaccg
     1321 ccgagtcgct cgcgaagctg aagcccgcct tccgcaagga aggcacgatc accgccggca
     1381 attcgccggg actcaattcg ggcgcctctg cgatgctgct ggccgaacgc ggctgggccg
     1441 agaagaacgg actggtgccg atggcgcgtc tggccagctt cggcctcgca gccgtcgagc
     1501 cggacttctt cgggctgggc ccggcgcctt cggtgcatca ggcgctggcg cgcgccggct
     1561 ggtcggtggc cgacgtcgat cgcgtcgaga tcaacgaggc tttcgctgcg atcgcgctgt
     1621 cgtgcctgcg cgagatcggc ttcgccgagg acgtggtcaa tgtcgagggc ggggcgatcg
     1681 cgcacgggca cccgatcggc gccagcggtg ccatcctgtc cacccggctg atgtactcga
     1741 tgaagcgcga tggcctcagg cgcggcatcg tcacgttgtg catcggcggc gggcagggca
     1801 tcgcgctcgc catcgagatg ctgtgacggt ggcggcatcc gcaggagtcg attccgcgac
     1861 cgagcggcca gggttcctgg cgaaggagcg cacgatcgcc gtgccgggct tcaaccggtg
     1921 gctggtgccg ccggccgcgc tggcgatcca cctgtgcatc ggcatggcct atggtttctc
     1981 ggtgttctgg ctgccgctgt cgaaggccat cggcatccag aagtcgatgg tctgcccggc
     2041 cgacatgagc ctgctggccg aactgttcac caccgcgtgc gactggcgca tctccagcct
     2101 gggctggatg tacaccttgt tcttcgtgct gctgggttcg tcggcggcca tctggggcgg
     2161 ctggctcgaa cgggccggtc cgcgcaaggc ggccttcgtc gcggcgctgt gctggtgcgg
     2221 ggggctcgtc atctcggccg tgggcgtgaa gacgcaccag ctctggctgc tctggctcgg
     2281 agccggcttc atcggaggca tcggactggg cctgggctac atctcgcccg tgtcgacgct
     2341 gatcaagtgg ttccccgacc ggcggggcat ggccaccggc atggcgatca tgggattcgg
     2401 aggcggcgcc atgatcggct cgccgctggc ggccgaactg atgaagcact tcgccaccgc
     2461 aaccgatgtc ggcgtggcat cgaccttcct cgtgctcgct gcgatctatc tcgtgttcat
     2521 gatgggcggt gcgctggcgt accgcgtgcc gcccagcggc tggaagccgg cgggctggac
     2581 gccgcccgcc gcgaaggtga acgacgcgat ggtcacccgg cgtcacgtcc acgtgaagaa
     2641 ggtctggggc atcccgcagt tctggctgct gtggatggtg ctgtgcatga acgtgtcggc
     2701 cggcatcggc gtgatcggca tggccagccc gatgctgcag gaggtgttcg gcggtcagct
     2761 gatcggccag ccgctgcact tcagcgaact cgatgccacc cagctgggcg cgatcgccgg
     2821 cgtggccgcg ggtttcacgg cgctgctgag cctgttcaac atcggcggcc gcttcttctg
     2881 ggccagcctg tcggacctgc tgggtcgcaa gtcgacctat gcggtgttct tcgtgctggg
     2941 cgccgcgctg tacgccagta tcccggccag cgccggcgcc gggcggcagc tgctgttcgt
     3001 tgccgcgttc tgcatcatcg tcagcatgta tggcgggggc tttgccgccg tgccggccta
     3061 cctggccgac ctgttcggca cgcagatggt gggcgcgatc cacggccggc tg
//
LOCUS       DAOORV010000300         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_38079_length_3078_cov_8.154482, whole genome shotgun sequence.
ACCESSION   DAOORV010000300 DAOORV010000000
VERSION     DAOORV010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38079_length_3078_cov_8.154482"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKV56_19495"
     CDS             complement(<1..826)
                     /locus_tag="PKV56_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004546048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOX70031.1"
                     /translation="MKLQFEHNLDYQLAAIDAVCDLFSGQEVCRTEFTVTAPRSVYLG
                     EGTQSALPGMSGDGVQLGLGLAESDLGIGNRLQLDSQDLLANLNKVQLRHGLKPSDSL
                     TSRDFTVEMETGTGKTYVYLRTIFELNKRYGFTKFVIVVPSVAIKEGVYKSLQIMAPH
                     FRSLYSGQPFEYFLYDSAKLNQVRNFATSQHLQVMVVTVGAINKKDVNNLYKDSEKTG
                     GEKPIDLIRATQPVLIVDEPQSVDGGLEGRGKEALDAMNPLCTLRYSATHVDKHHMV"
     gene            complement(885..1601)
                     /locus_tag="PKV56_19500"
     CDS             complement(885..1601)
                     /locus_tag="PKV56_19500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02646 family protein"
                     /protein_id="HOX70032.1"
                     /translation="MRSVSKGGGHYALAARWAVHQTELAAGTASKDAWSNYGHRAGTR
                     TSCLAEQFSLCAYAELVLDETDLGMHLDHVKPKSKFPHDTFSHNNLLLSAIDDVGRRQ
                     LTAQDVFGGHARGNRYHPTWFIHPLKPDSRRFFHYASDGRMEPRRGLSTRDRRRAVYT
                     IGVMNLNAPVLVNRRRRWLEEIELEIDKLLGDSVALEYFAMVELCPTGDRLRPFHSAV
                     RTRFGALGEAVLAKHCPLCA"
     gene            complement(1598..3004)
                     /locus_tag="PKV56_19505"
     CDS             complement(1598..3004)
                     /locus_tag="PKV56_19505"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX70033.1"
                     /translation="MTEQLQSPEGPRTYLERIELSNYRCFAHLAVPMHPQLTVLIARN
                     GSGKTSVMDAIAIAFGTFVGCFLAGTGIGANHRDVRVRLTNPALREMEPQYPLTIKAV
                     GTVNGRHLNWSRNVNSSKSGTTIKDAKPLTSLGEGLQRAVTDNEPVVLPVLAYYGTGR
                     LWKQKKVTEKKVFSSEFHSRTSGYQDCLDPASSYKFFLDWFRYAASADAEAVNRQREQ
                     NVSQSDLVESAYAGLIAAVRGAVDECLKSSGWQNLRYSFTHQSVVMEHPDHGILEVGQ
                     LSDGVRNMIALVADVAYRMVRLNSHFGSEAAKRTPGIVMIDEVDMHLHPEWQQQVLAS
                     LRLAFPAIQLIVTTHSPQVLSTVRKEHIRVLGINVDGENVASEPLARTYGRSNADVLQ
                     GTMGVTPEPPTEGSDLLRRYLNLVEQASLDDPDIPGWRAELVEIFGSDHPSLIRADMV
                     VRRRRALGDGALPPESNR"
BASE COUNT          550 a          958 c          954 g          616 t
ORIGIN      
        1 agaccatgtg gtgcttgtcg acgtgcgtgg ccgagtagcg cagcgtgcac agcggattca
       61 tggcatccag cgcctctttg ccgcgccctt ccaggccacc gtccacactc tgcggctcgt
      121 cgacgatcag cacgggctgc gtagcgcgga tcagatcgat gggcttctcg ccaccggtct
      181 tctcgctgtc cttgtagagg ttgttgacgt ccttcttgtt gatggcgccc accgtcacca
      241 ccatcacctg caagtgctgg ctggtagcga agttgcgcac ctggttcagc ttggcggagt
      301 cgtagaggaa gtactcgaag ggctggccgc tgtagagcga ccggaagtgc ggcgccatga
      361 tctgcaagct cttgtagacg ccttccttga tggccaccga cggcacgacg atcacgaact
      421 tggtgaagcc gtagcgcttg ttcagttcga agatcgtgcg caggtagaca taggtcttgc
      481 ccgtgcccgt ttccatctcc accgtgaagt cacgcgacgt caggctgtcg ctgggcttca
      541 agccatggcg cagctgcacc ttgttcaagt tggccagcag atcttggctg tccagctgga
      601 ggcggttgcc gatgcccagg tcggactcgg ccaagcccag gccgagctgc acgccgtcac
      661 ccgacatgcc gggtagcgca ctctgtgttc cttctcccag gtagaccgag cgcggtgccg
      721 tcaccgtgaa ctcggttcgg cagacctctt gacccgagaa gaggtcgcac accgcatcga
      781 tggcggcgag ctggtagtca aggttgtgtt cgaactggag cttcatggtg tgctgacaat
      841 ctatgttggg acacgcgctg ctcggcccat acgtgctgct aggtctacgc gcacagcgga
      901 cagtgtttgg ctagcaccgc ttccccgagc gctccgaacc gtgttcgtac cgcgctgtgg
      961 aacggccgca gtcgatcccc cgtcgggcac agctcgacca tcgcaaagta ctcaagcgcc
     1021 acgctgtcgc ccagcagctt gtcgatctca agttcgattt cctcaagcca gcgccgccgc
     1081 ctgttgacga ggacgggcgc gttcaagttc atgacgccga tcgtgtacac ggcgcgacgg
     1141 cgatcacgag tcgacaaccc gcgtcgcggc tccatgcgcc catcgctggc gtagtggaag
     1201 aagcgacggc tgtcaggctt gagcggatgg atgaaccaag ttgggtgata ccgattgcca
     1261 cgtgcatgtc cgccgaacac gtcctgcgct gtcagttgac gcctaccgac atcgtcaatt
     1321 gccgacagca gcaagttgtt gtggctgaac gtgtcgtgcg gaaacttcga cttcggcttg
     1381 acatggtcaa gatgcatgcc caagtcggtt tcgtccagaa ccagctcggc ataggcacac
     1441 agcgagaact gttcggcaag gcacgacgtc cgcgtccccg cacgatgccc gtagttggac
     1501 caagcgtcct ttgacgccgt cccggcagca agctccgttt gatgcacagc ccaccgcgcg
     1561 gcgagcgcat agtgcccgcc gcccttcgac accgacctca tcgattcgac tccggtggca
     1621 gcgctccatc ccccaacgcg cgacgacgtc gcaccaccat gtcagctcga atcagtgagg
     1681 gatgatcact gccgaagatc tcgaccaact ctgcccgcca acccggaatg tccggatcgt
     1741 cgagcgaggc ctgctccacg aggttcaggt agcggcgaag caggtcgctg ccttcggtcg
     1801 gtggctctgg ggtaacgccc atcgtcccct gaagcacatc ggcattgctg cgaccgtagg
     1861 ttcgcgcaag gggttcgctc gcgacattct cgccgtcgac gttgataccg aggacgcgga
     1921 tgtgctcctt gcgtaccgtg gatagcacct gcgggctgtg cgtcgtgacg atcagctgaa
     1981 tggcagggaa cgccaggcgc agcgatgcca gcacttgttg ctgccactcc ggatgcaggt
     2041 gcatgtcgac ctcgtcgatc atgacgatgc cgggggtgcg cttggcggcc tcggagccga
     2101 agtgcgagtt gaggcggacc atgcggtagg ccacgtccgc caccagcgcg atcatgttgc
     2161 gcacgccgtc tgaaagctga ccgacttcca ggatgccgtg gtccggatgc tccatcacga
     2221 cggactggtg cgtgaagctg tatcgcaggt tctgccagcc agatgactta aggcactcgt
     2281 ccacagcgcc gcggaccgcg gcgatgaggc cggcatatgc agactccacc agatcggact
     2341 gggaaacgtt ctgctcgcgc tgccggttca cggcctcagc gtcggcgctg gcggcgtagc
     2401 gaaaccagtc gaggaagaac ttgtagctgg aagcggggtc aaggcagtct tggtagcccg
     2461 aggtgcgcga gtgaaactca cttgagaaga ccttcttctc ggtcaccttc ttctgcttcc
     2521 acagccgccc ggtgccgtag tacgccagta ccggcagaac caccggctcg ttgtccgtca
     2581 cggctcgctg caagccttcc ccaagcgacg tcagcggctt cgcatccttg atcgtcgtgc
     2641 ccgatttgga gctgttgacg ttgcgcgacc aattgaggtg gcgcccgttg acagtgccca
     2701 cggccttgat cgtcaacggg tactgcggct ccatctctcg cagtgcgggg ttggtcaggc
     2761 gcactcgcac atcacggtga ttcgcgccga tgccggtgcc cgccaggaaa caaccaacga
     2821 atgtgccgaa tgcgatggcg atggcgtcca ttaccgatgt cttgcccgag ccgttgcgcg
     2881 cgatcaggac ggtcagctga gggtgcatgg gcacagccag gtgtgcgaag cacctgtagt
     2941 tgctcagctc gatccgctct aggtaggtac gtggcccttc agggctctgt agttgttctg
     3001 tcatgagttg accttcacgc taaatgctgc gcacgtcgtc caagccgtgc tggctgagga
     3061 tggcggccag gttggtct
//
LOCUS       DAOORV010000301         3056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_38491_length_3056_cov_11.098967, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000301 DAOORV010000000
VERSION     DAOORV010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38491_length_3056_cov_11.098967"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="PKV56_19510"
     CDS             complement(<1..2042)
                     /locus_tag="PKV56_19510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOX70034.1"
                     /translation="MQPVDKASPEAQSANLVAGNLAQLKAMFPAAFTEGRVDFEVLKQ
                     LLGAAVDEREEKYGLNWHGKRRARQLALTPSTGTLRPCPEESVDWDTTQNLFIEGDNL
                     EVLKLLQKSYAGKVKLIYIDPPYNTGKDFVYPDNFQDSIKNYLELTGQVEGGQKISSN
                     TEASGRFHTDWLNMLYPRLKLARALLRRDGVVFISIDDRELHHLRVVANEVFGEENFV
                     ACVTVVNNLKGRNDKKHIAACHEYVVIYAVPGFVSHGLPLTDEQRAAFKFSDEKGNKY
                     ALRDLRKRGGPDRREDRPNMFFPIFWDETTGKCSLERKAETDVEVVPLRGDGSEGRWR
                     WGTDKVAQHIEWMHPKRSDRKGRLDVEHRVYLDASIAIEDDPEDDDDEEDEGSVERTS
                     KPKSIWLGGEFSTDSAKRSLKELIPGDAFDFPKSLDFLRTCIQLGTRGNDIVLDLFAG
                     SAGTGQAVLQQNAIDGQSRRHILVQLPEPLDPADKAQKAAAAVCAKLGLKLNIAELSK
                     ERLRRAGVKIRGEGEILQQDLGFRVFRLDNSNIRAWNPDPSDLQTTLDEHTEHLVHGR
                     SEQDVLYELLLKLGLDLAVPMESRQIAGKTVHSIGAGALMACLSDGIDRDVAEPLALG
                     IAAWQKAQAQALQGDDDNAKPVSTKLVFKDSAFADDVAKTNLAAILSQHGLDDVRS"
     gene            complement(2095..2991)
                     /locus_tag="PKV56_19515"
     CDS             complement(2095..2991)
                     /locus_tag="PKV56_19515"
                     /inference="COORDINATES: protein motif:HMM:NF025693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HOX70035.1"
                     /translation="MTAQVLIDAFRLPAQALVAQRIPKKLLVEQGAPTAADKRLINDT
                     IDELWWQATLKPGTVGVPAFHAATVPATPATTPTVGMGSAADGASEGGAASANATEAT
                     DVIELALVSAHLRPPTREAQARRLTQLIHRAIPYPVLLVTQASTGTVLSVAHKRASQG
                     EAGKWVVADGADTHAFNPAQPTTAETEFMASLALQQLPPSALADLRALYQAVADRITA
                     LAVAQVTGHFKAPEAATAAPAARTAAQRQALAERSRLLDQLASARSTAAKARQMSQRV
                     ELNLQIQRLLRQLQEIQEGLAT"
BASE COUNT          484 a          951 c          989 g          632 t
ORIGIN      
        1 atgctgcgca cgtcgtccaa gccgtgctgg ctgaggatgg cggccaggtt ggtcttggcc
       61 acgtcgtcgg caaaggcgct gtccttgaag acgagcttgg tgctgacagg cttggcgttg
      121 tcgtcgtcgc cttgcagcgc ctgggcctgg gccttctgcc acgccgcgat gcccagagcg
      181 agcggctctg ccacgtcgcg gtcgatgccg tccgaaaggc aggccatcag ggcgccggcg
      241 ccgatgctgt gcaccgtctt gccggcgatc tggcgcgact ccatgggcac cgcgaggtcg
      301 aggccgagct tcaggagcaa ctcatagaga acatcctgct cgcttcgacc atgcaccaag
      361 tgttccgtgt gctcgtccaa ggtggtctgc agatcgcttg gatctgggtt ccaggctcgg
      421 atatttgagt tgtccagtcg gaagacgcgg aatccaaggt cttgctgcag gatttcgcct
      481 tctccccgaa tctttacgcc agcccggcga agtcgctcct ttgaaagctc cgcgatgttg
      541 agcttcaggc caagtttggc gcaaacagcc gctgcggcct tctgcgcctt gtcggccggg
      601 tcgagcggtt ctggcagctg aacgaggatg tgtcgacgcg actgaccgtc aatggcgttc
      661 tgctgcagaa cagcttgccc agttccggcc gacccggcga agaggtcgag cacgatgtcg
      721 ttcccacgcg tgcccaactg gatgcaggtt ctcagaaaat ctaatgactt cgggaaatcg
      781 aatgcatccc cagggatcaa ctccttgagc gaccgcttgg cactgtctgt tgaaaactca
      841 ccgcccagcc agatcgactt aggcttcgaa gtccgctcga ccgagccttc gtcttcctcg
      901 tcgtcgtcat cttcggggtc gtcttctatt gcgatggatg cgtcgagata gacgcgatgc
      961 tcaacgtcga ggcgaccctt gcgatcagac cgcttcgggt gcatccactc gatgtgctga
     1021 gcgaccttgt cagtgcccca gcgccagcgg ccttcacttc catcgccgcg cagaggaacg
     1081 acctcgacgt ccgtctctgc cttgcgctcc agagaacact tcccggtcgt ttcgtcccag
     1141 aagattggga agaacatgtt cggccggtct tctcgccgat caggtccgcc acgcttgcgg
     1201 agatctcgta gcgcgtactt gtttcccttt tcatcactga acttgaaggc cgcgcgttgc
     1261 tcatccgtca gcggtagacc gtgcgagacg aagcctggga cggcgtagat gacgacatac
     1321 tcgtggcaag cagcaatgtg cttcttgtcg tttcgcccct ttaggttatt cacgaccgtc
     1381 acgcaggcga caaaattttc ctcgccgaag acctcgttgg cgactacacg caggtggtgt
     1441 agctctcgat cgtcgatgga gataaagacg accccatctc gacgaagaag cgcacgcgcc
     1501 agcttcaagc gcgggtagag catgttcagc cagtctgtgt gaaaccgccc cgatgcctcg
     1561 gtgttcgaac tgatcttctg cccgccctcc acctgtcccg tcagctctag gtagttcttg
     1621 atgctgtcct gaaagttgtc cgggtagacg aagtccttgc cggtgttgta cggcgggtcg
     1681 atgtagatga gcttcacctt gccggcatag cttttctgca gcagcttcag cacctccagg
     1741 ttgtcacctt cgatgaacag gttctgcgtg gtgtcccagt cgacgctctc ttccgggcag
     1801 gggcgcagcg tgcccgtgct gggcgtcagc gccagctggc gagcgcggcg cttgccgtgc
     1861 cagttcaggc cgtacttctc ctcgcgctcg tccaccgcag cgcccagcaa ctgtttgagc
     1921 acctcgaagt cgacccggcc ttcggtgaag gccgcgggga acatggcctt cagctgcgcg
     1981 aggttgcccg ccacaaggtt ggcgctctgg gcctcggggc tcgccttgtc gacgggctgg
     2041 atggttgctg ggggtcgggg tgcagtcatg ggcaaagtct gaggttgcgg tggctcatgt
     2101 ggcgagccct tcctggattt cttgaagttg gcgcagcaga cgctggatct gcagattgag
     2161 ttcaacgcgc tggctcatct gtcgggcctt ggccgccgtg cttcgcgcag aagccagctg
     2221 atcgagcagc cggctgcgtt cggccagcgc ttggcgctgt gccgcggtgc gcgcagccgg
     2281 cgcagcggtt gctgcctcgg gcgccttgaa gtggcccgtc acctgggcca cggccagcgc
     2341 ggtgatgcgg tcggccacgg cctggtacag cgcccgcaga tccgccaggg cactgggtgg
     2401 caactgctgc agggccagac tggccatgaa ctcagtttcg gcagtagtgg gctgcgcggg
     2461 gttgaaggcg tgcgtgtctg caccgtcggc caccacccac ttgccggctt cgccctgcga
     2521 agcccgcttg tgcgccactg acagcacagt gccggtggag gcctgcgtca cgagcagcac
     2581 cgggtacggg atcgcccggt ggatgagttg cgtgagtcgc cgggcctgcg cctcgcgagt
     2641 gggtgggcgc aggtgcgccg agaccagtgc gagctcgatc acatcggtgg cttccgtggc
     2701 attggcgctg gcagcgccac cctcagaagc gccgtctgct gccgagccca tcccgaccgt
     2761 cggtgtggtt gctggcgtgg cgggcaccgt cgcagcgtgg aaggccggca cgcccaccgt
     2821 gcccggcttg agggtggcct gccaccacag ctcgtcgatg gtgtcgttga tcaggcgctt
     2881 gtcggcagcc gtgggggcac cctgctcgac caagagcttc ttcgggatgc gctgcgccac
     2941 cagcgcttgt gccggcaggc gaaaggcatc gatgagcacc tgggccgtca tcttgtggcg
     3001 gctacagcag gtcaggccga ttggcctgca gccagctgcg gacgtggctg aacagc
//
LOCUS       DAOORV010000302         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_40077_length_2969_cov_14.072752, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000302 DAOORV010000000
VERSION     DAOORV010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40077_length_2969_cov_14.072752"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1298
                     /locus_tag="PKV56_19520"
     CDS             363..1298
                     /locus_tag="PKV56_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOX70036.1"
                     /translation="MDPPEHAAWRALVDGFFAPAQIAALEPRVRAIADAVVAALPRGT
                     VFDAVAELGRPFAVRAQTAWLGWQGIEDELLGWMTDNHAATRCGDTARNAAVAAAFDE
                     IIRRQLRRRRTPDAAASGDPTTALLSATREGQPLSEADIVSILRNWTAGDLASMAAAL
                     GVVVWFLAAHPDVQQNLRNRPDDLAPAIDEMLRIDDPFLVNRRVTTRAVRIGEHELAA
                     GTRVYLNWTSANRDEAVFGDPDAYQPARNAPHNLVYGAGIHVCPGRPLATMELVVAVQ
                     SLLTATALIEPAPDAAPERETYPLGGWRRVPVLLR"
     gene            1330..2403
                     /locus_tag="PKV56_19525"
     CDS             1330..2403
                     /locus_tag="PKV56_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HOX70037.1"
                     /translation="MTMTDTSHSIAAPSTSGLAGLSPAYFGMVMATGIVSIAAHLLGH
                     PLLARALFALNLVAYTVLCLLNLLRALRHRQRIMGDLFDHLRGPGFFTSVAATSIVGS
                     QFLLLTDDSRVAGALWAVAVVLWVALTYTIFAGLTIKQNKPSLDKGINGAWLLAVVAT
                     QSIAALSALLAARLGQPVRLELNFLALSMWLWGGMLYIWMMSLIFYRYTFFTLEPGDL
                     SPPYWINMGAMAISTLAGSLLILNAPDAPFLLSVLPFLKGFTVFYWATGTWWIPMLLV
                     LALWRHVYRRFPLRYDPLYWGAVFPLGMYAASTERMIEAMGIGFLDAVPRVFFPIAVA
                     AWFAAFLGLASGLLGRTRHAPVP"
     gene            2441..>2969
                     /locus_tag="PKV56_19530"
     CDS             2441..>2969
                     /locus_tag="PKV56_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOX70038.1"
                     /translation="MTSSLAIRHVAFEDLGTLAPVLDHAGIAPRYLDGWSPELAHVDP
                     AAPDLVVVLGGPIGVYEVAAYPWLAHEIAWLRTRLLAGRPTLGICLGAQLIAAALGAR
                     VYPGPSKELGWMPLQLSAAGRASALAPLDGELTSMLHWHGDTFDLPEGAQLLASTASY
                     RHQAYRWQQHVLGLQC"
BASE COUNT          399 a         1061 c          972 g          537 t
ORIGIN      
        1 gtgctggaag caggcagagc tttggtgatg tcatgggtta cgtaattctc aatagaacgt
       61 tgatgcctcg gcttgggccg gcgttgttga cgcatctcat accgtgggta ctgcagttca
      121 ccgcgggcac ttcgcgtgag aacatctcct gcatgcatcc atcgattccg gcctgggagc
      181 cgctgtcgtc cgaggctctc gccgaccagc tgggaaccta tgatcgccag cgcaccgcct
      241 gcgcgctggc ccggtcgccc cgcggggtca cgctgttccg gcacgccgac gttgttgcgg
      301 cggcgcacga cccacagacc ttctcgtcag ccttgccgtc gaggcgcgcg gtgccgaacg
      361 gcatggatcc gcccgaacat gcagcctggc gcgcactggt cgacggtttc ttcgcacctg
      421 cccagatcgc ggcgctggag ccccgcgtgc gcgcgatcgc cgatgccgtc gtggcggcgc
      481 tgccgcgcgg aacagtattc gacgcagtcg ccgaactcgg ccgcccgttc gccgtgcgcg
      541 cccagacggc ctggctggga tggcagggca tcgaggacga actgctcggc tggatgaccg
      601 acaaccacgc cgccacgcgc tgcggcgaca ccgcgcgcaa cgccgcggtt gccgctgcgt
      661 tcgacgagat catccgcagg cagttgcgca ggcgccgtac cccggacgcg gccgcgtccg
      721 gcgacccgac caccgcactg ctcagtgcaa cgcgagaggg gcagccgctg agcgaagcgg
      781 acatcgtctc catcctgcgc aactggaccg ccggtgacct cgcgtcgatg gcggcggccc
      841 tgggtgtcgt cgtctggttc cttgccgccc atccggacgt gcagcagaac ttgcgtaacc
      901 gccccgacga cctcgcgccg gcgatcgacg agatgctgcg catcgacgac cccttcctcg
      961 tcaaccggcg cgtgacgaca cgtgccgtgc gcatcggcga gcacgaactc gccgccggca
     1021 cccgcgtgta cctcaactgg acctcggcca atcgcgacga ggccgtcttc ggcgatcccg
     1081 acgcctacca gcccgcgcgc aatgcgccgc acaacctcgt ctacggcgcc ggtatccatg
     1141 tctgtccggg acgtccgctg gcgacgatgg agttggtcgt cgccgtgcag tcgctgttga
     1201 ccgccacggc gctcatcgag cccgcgcccg acgccgcgcc cgagcgcgag acctatccgc
     1261 tgggcggctg gcgccgggtg ccggtcctcc tgcgctgacg ggctcgtcat cgcgcgacgc
     1321 cgatggccca tgaccatgac ggacaccagc cactccatcg cggcgccctc gacgtccggc
     1381 ttggccggct tgtcgcccgc ttacttcggc atggtgatgg cgaccggcat cgtgtcgatc
     1441 gccgcccatc tgctgggcca tccactgctc gcccgggcgc tgtttgcgct gaacctggtc
     1501 gcctacacgg tgctgtgcct gctgaacctg ctgcgcgcgc tgcgtcatcg ccagcgcatc
     1561 atgggtgacc tgttcgatca cctgcgcgga ccaggcttct tcaccagcgt cgcggccacc
     1621 agcattgtcg gcagccagtt cctgctgctg accgacgaca gccgtgtggc cggtgcgctg
     1681 tgggccgtgg cggtggtgct gtgggtcgcc ctgacctaca cgatcttcgc gggcctcacg
     1741 atcaagcaga acaagccgtc gctggacaag ggcatcaacg gtgcttggct tctcgcggtg
     1801 gtcgccacgc agtcgatcgc ggccctgagc gcgctgctcg ccgcgcggtt ggggcagccg
     1861 gtgcgactgg agctgaactt cctggcattg tcgatgtggc tgtggggagg gatgctctac
     1921 atctggatga tgtcgctgat tttctaccgc tacaccttct tcacgctgga gcccggcgac
     1981 ctctcgccgc cctactggat caacatgggc gcgatggcga tctcgactct tgcggggtcg
     2041 ctgctcatcc tcaacgcgcc cgacgcgccg ttcctgctgt cggtgctgcc cttcctgaag
     2101 gggttcacgg tgttctactg ggctaccggc acctggtgga ttccgatgct gctggtgctg
     2161 gcgctgtggc gccatgtcta ccggcgcttt ccgctgcgct acgacccgct gtactggggc
     2221 gcggtcttcc cgctcggcat gtacgcggcg agcacggagc gcatgatcga ggcgatgggt
     2281 atcgggttcc tcgatgccgt tccgcgcgtg ttcttcccga tcgccgtagc ggcctggttc
     2341 gcggctttcc tagggctggc ttctgggttg ctgggccgga cgcggcatgc cccagtgcca
     2401 tgacccccac agcgggcaga cttctggatc gagtttcatc gtgacaagca gcctcgccat
     2461 ccggcacgtc gcattcgagg acctcggcac cctcgctccc gtcttggacc acgccggcat
     2521 cgcaccccgc tatctggacg gctggagccc ggagctggcc cacgtcgatc ccgccgcccc
     2581 ggacctggtc gtggtgctgg gcggccccat cggtgtctac gaggtcgccg cctacccctg
     2641 gctcgcgcac gagatcgcgt ggctgcgaac gcgcctgctg gccggtcgac ccacgctcgg
     2701 catctgcctg ggggcccagc tgatcgccgc cgcgctgggc gctcgcgtgt accccggccc
     2761 gtcgaaggaa ctcggctgga tgccgctgca actgagcgcc gcagggcgcg cttcggccct
     2821 ggctccgctg gacggcgagt tgacctcgat gctgcactgg cacggcgaca ccttcgacct
     2881 gcccgaaggc gctcagctgc tggccagcac ggcctcgtac cgccatcagg cctaccggtg
     2941 gcagcagcat gtgctgggcc tgcaatgcc
//
LOCUS       DAOORV010000303         2944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_40555_length_2944_cov_10.659398, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000303 DAOORV010000000
VERSION     DAOORV010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40555_length_2944_cov_10.659398"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..802)
                     /locus_tag="PKV56_19535"
     CDS             complement(329..802)
                     /locus_tag="PKV56_19535"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HOX70039.1"
                     /translation="MTAEFSDCERFRYSLSEVWETGRPLVMFLMMNPSVAGPEHADPT
                     LIKTGKYARAWGYGGQLVGNVHAYRVTDSGLLVEAEDPVGPKNDAVLLRMARRAEVVV
                     LAFGLPPKPLRPRAATVIEMLRGTVPLKYLHKTKNGTPRHPLYLRRDLRPIDFIA"
     gene            complement(1068..1835)
                     /locus_tag="PKV56_19540"
     CDS             complement(1068..1835)
                     /locus_tag="PKV56_19540"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOX70040.1"
                     /translation="MRAVAIALGFVAGMPTAASAWGSEGHRLVAEVAEGRLTAAARVE
                     IDRLLALEPGSTLASISTWADEVRSPSTASWHYVNFPRDGDCRYEPDRMCLQGSCVVG
                     AIERQTALLASNAPDEERLKALKYVVHFVADVHQPLHAGFADDRGGNGYQLQAYGRGT
                     NLHALWDSGFIRQWPGGAASLQAAVDAAVHPTDAGPGARAWAEESCRVVSAEGFYPQG
                     HKLGDDYPPSVRAVLVQQLAIAGSRLAAVLNRSLGQR"
     gene            complement(1927..2151)
                     /locus_tag="PKV56_19545"
     CDS             complement(1927..2151)
                     /locus_tag="PKV56_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70041.1"
                     /translation="MKAKQVEVINMRLDFMSMVLAVLARSLPPREAAHAVQVIGERVV
                     EQLGRDPVSESAEEAVAADLAPIIAALQQR"
     gene            complement(2297..2767)
                     /locus_tag="PKV56_19550"
     CDS             complement(2297..2767)
                     /locus_tag="PKV56_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70042.1"
                     /translation="MSKPIIRYLRFHCPTPTSMPQANRDGALAVAAIREDAQLAQMLL
                     IDMRRWDRNDGASLRDALGVLVPAAHRRLVAGFGIPMADTLIVEWDGGDVFTRLSVEG
                     EAARQGCDDTRACALRSGLPGGRSSFLAWARPAGEAMLEKAEAVAGGVWAGVEG"
BASE COUNT          456 a         1065 c          918 g          505 t
ORIGIN      
        1 gttctgcgtt gcactgtgcg tgtctgcttg ggtccgcagc acggccgaga gtttctcgca
       61 cggcggcctt cgacgcacga aattcacgca tcgcaactct ggagacccga cccgcactgc
      121 ggcggtcgaa ccctgcacac cgcaggtccc gcaatgcaat cgggcccgcc gcagctgagt
      181 gccacggcgt caccggcccc acctgccgtg tccgtgccgg ccacctccat gcatcaggcc
      241 tggcagtcct tcaacaactg cagtctgcta ggaccaaccg ccgtctggcg acgaaaggct
      301 gccggatcgt tcgcggtcgg cgggaccgtc aggcgatgaa gtcgatcggc cgtaggtctc
      361 gtcgcaggta cagcggatga cgaggcgtcc cgttcttcgt cttgtgcagg tacttcagcg
      421 gaaccgtccc tcgcagcatc tcgatcacgg tggctgcgcg cggacgcaga ggcttcgggg
      481 gcaggccaaa ggcgagaaca acgacctcgg ctcttcgcgc catgcggagc agaaccgcgt
      541 cgttcttcgg ccccaccgga tcttcggctt cgacgaggag accgctgtcg gtgacccggt
      601 atgcatggac gttgccgacc aactggccgc cgtagcccca ggctctcgcg tacttgccgg
      661 tcttgatgag cgtcgggtcg gcatgctcag ggccggccac ggacggattc atcatcagga
      721 acatgaccag gggtcgccct gtctcccata cctcggacag gctgtagcgg aagcgctcgc
      781 agtccgagaa ctcggccgtc acgaccgaat ccggcggcca tctcggcttt ctcctgccgc
      841 ctggatcgtg cgtcatgtct gctcccgcct ccactgtcag cccatgatgg gcggggccgc
      901 agtcccggca cctccctgaa actcactcca tcctgccatg ggcagccctt tcaggtgatc
      961 agcccgtcga cgctcaggac gatctgtcga tcggcacccc tgagcgctgg cgcccacgga
     1021 ccctctgcct gtgtacccgg gccggacctt caggttgatc cgatcgacta gcgctggccg
     1081 aggcttcggt tgagcacggc ggccagcctc gatcctgcga tcgccagttg ctgcacgagc
     1141 acagcgcgaa cactcggcgg atagtcgtcg cccaacttgt ggccctgtgg gtagaaccct
     1201 tccgcgctca ccacgcgaca cgactcctcg gcccaggcgc gtgcgccggg accggcatcg
     1261 gtcggatgca ccgcggcatc taccgctgcc tgcagcgacg cagctccacc cggccattgc
     1321 ctgatgaagc cactgtccca gagtgcatgc agattggtgc cacggccgta ggcctggagt
     1381 tggtagccgt tgccgccacg gtcatccgca aagccggcgt gcagtggctg gtgcacgtcg
     1441 gccacgaagt gcacgacgta cttcaacgcc ttcagtcgtt cctcgtcggg cgcgttggag
     1501 gcgagcagcg cggtctgtct ctcgatcgcg ccgacgacgc aggacccttg caggcacatt
     1561 cgatcgggct cgtaccggca gtcgccgtca cgggggaagt tgacgtagtg ccaggaggcg
     1621 gtggacgggg agcgaacctc gtcggcccag gtcgagatcg atgccagagt cgacccgggt
     1681 tcgagggcca gcaatcggtc gatctcgacc ctcgccgccg cagtcaaccg cccttccgct
     1741 acctcggcga ccagacggtg gccctcggat ccccacgcgg atgctgctgt gggcatcccg
     1801 gccacgaagc cgagcgcgat tgcgacggca cgcacgcctt tggcgacaac gcacccccga
     1861 aggcgcccct gcttccactc cgctcggcag aacatcctgt tcccgttccg gcccatcgga
     1921 cctgtctcag cgctgctgaa gggccgcgat tatcggcgcg aggtccgcgg cgaccgcttc
     1981 ctcagcgctt tccgagaccg ggtcgcggcc cagctgttcg acgacccgct ccccgatcac
     2041 ctggaccgca tgggcggctt cccggggcgg cagcgacctc gccagcaccg ccaggaccat
     2101 gctcatgaag tccagccgca tgttgatcac ttccacttgc ttcgccttca tggttgcctc
     2161 cgtgaccggt acgtcaccgg catgggcggc atggtttccg gtgcggcaac ggttgtcgcc
     2221 caccattggc gtgcaccccc accttctggg ggaccgctcc ttctgacgag gggcacccct
     2281 tggtgggatg ggcgcttcac ccttccaccc ccgcccacac accgcccgct acggcttccg
     2341 ccttctcgag cattgcctcc cctgccggcc tcgcccaggc aaggaaggaa gaccgcccgc
     2401 ccggaaggcc gcttcgcagt gcgcaggccc gggtgtcatc gcagccctgc cgtgccgcct
     2461 cgccttcgac ggacagcctg gtgaacacgt ccccgccgtc ccactccacg atcaaggtgt
     2521 cggccatcgg aatgccgaag ccggcgacga gacgacggtg ggccgcaggc accaggactc
     2581 ccagcgcatc cctcaggctg gcgccgtcgt tgcgatccca tcgccgcatg tcgatcagca
     2641 gcatctgtgc gagctgggcg tcctcgcgga tggcggccac cgccagcgcc ccgtcgcggt
     2701 tcgcctgcgg catcgaggtc ggcgtcgggc agtggaaccg caggtatcgg atgatgggct
     2761 tcgacatggg gagcgccttc gcgacaggac tccatgttcc ggccgtgcgg ccctgcatca
     2821 acccgtcagc ccggcagcag tcgcctgtgg cgcagccaat cgtcgaagga ctcgttcagc
     2881 cggatcgccg cgcgtgcccg ggtcagcgcc acgtacagga tgttgacctc ctcctgctcc
     2941 acct
//
LOCUS       DAOORV010000304         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_41274_length_2905_cov_12.886316, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000304 DAOORV010000000
VERSION     DAOORV010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41274_length_2905_cov_12.886316"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKV56_19555"
     CDS             <1..309
                     /locus_tag="PKV56_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70043.1"
                     /translation="HEFDVGPGDGVYFPSTSPHMTRSETDWTTPGDGVSISIGVVFYT
                     DVTRRAAHLHILNRFLRKLGVVPRAPGERPWVDALKYPLAWALVSFKKSFRGYIRAGI
                     "
     gene            complement(315..1457)
                     /locus_tag="PKV56_19560"
     CDS             complement(315..1457)
                     /locus_tag="PKV56_19560"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOX70044.1"
                     /translation="MMLKRKGWILLLAAIVALALAAWVTPKVLRRLDMQGLRPVTVAK
                     GLEHPWAIAFLPDGRMLVTERPGRMRIVSANGALSEPLAGLLPVASGDEGGLLDVVLD
                     PGFSVNRTIYWSFSESSDSSPQARSTAVARGRLIDDRVADARVIFRQPVKREDGSHFG
                     SRLVFARDGSLFVGLGDRGARRDAQDVASAHGKILRMDVQGNALSDNPLASTPSALHQ
                     VWSWGHRNIQGMAFHPETGELWATDHGPQGGDELNVIERGKNYGWPTITYGTEYADSA
                     KIGEGTAKSGMEQPVTWWGPISIAPSGMAFLTSDRYPGWKGQMFIGNLRGRALLRVKL
                     DGRKVIEQQRLVTGLLERIRDVRQGPDGWLYVVTNSPEGRIIRIER"
     gene            1806..>2905
                     /locus_tag="PKV56_19565"
     CDS             1806..>2905
                     /locus_tag="PKV56_19565"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOX70045.1"
                     /translation="MFRAFHHRAYAAVLLDAGMDALLGFLAVLIAAWTISAVPMSEFG
                     QTLSNPSVMLAASGFAVCMAMLQSFLGLYRQAGLSFASQLARLLVAMSIGGYLTYLVL
                     KEVGFDGHPSRLVGYSVANLIFAILVVRGTIVLVRAAQGSSRVLIVGTGPEAVSVAND
                     LRRSSRVSGEVIGFYPTPAVAPDQNIDGKLLLDARVPLEQLARELEIDSIIVATREQR
                     GGAMPMDQLVICRSMGIPVMDLAGFYERTHAEVPLDSLKASWLVYGPGFVQGRVRQAL
                     KRGFDIVTSSILLVLALPVMIIAAVAIKLDSRGPLIYRQERVGLGGRSFMCLKFRSMC
                     TDAERDGVARWATKNDARVTRVGRIIRKARID"
BASE COUNT          482 a          918 c          941 g          564 t
ORIGIN      
        1 cacgagttcg acgtaggccc gggcgatggc gtttacttcc cgagcacgtc gccgcacatg
       61 acgcgtagcg aaacggattg gacgacgccg ggtgacggcg tctcgatcag tatcggtgtc
      121 gtcttctaca cggatgtgac gcgcagggcg gcacacctgc acatcctgaa ccgcttcctg
      181 cgcaagctgg gggtggtacc ccgcgcgccg ggagagcggc cctgggtcga tgcactgaag
      241 tacccgctgg cctgggcgct ggtcagcttc aagaaatcgt ttcggggcta cattcgggcc
      301 ggcatttgaa ctgctcagcg ctcgattcgg atgatccggc cctcggggct gttggtgacg
      361 acataaagcc aaccgtcagg cccctggcga acatcgcgaa tcctctccag gaggccggtc
      421 accagtcgct gctgttcaat cactttgcgc ccgtcgagtt tgacgcgcag cagcgcacgg
      481 ccgcgtagat ttccgatgaa catctggcct ttccagcccg ggtaccggtc gctggtgagg
      541 aaggccatgc cactgggcgc gatggagatg ggcccccacc acgtgacggg ctgctccatg
      601 ccgctcttgg ccgtgccctc gccgattttg gcgctgtccg catattccgt cccgtaggtg
      661 attgtcggcc agccgtagtt ctttccgcgc tcgatgacgt tgagttcgtc accaccctga
      721 gggccatggt cggtagccca gagttcaccg gtttccggat gaaaggccat gccctggatg
      781 ttgcggtgcc cccaggacca cacctgatga agcgcgctcg gtgtcgacgc caaagggttg
      841 tcggatagcg cattgccctg cacgtccatg cgcagaatct ttccgtgcgc cgaagcgaca
      901 tcctgggcgt cccgccgggc tccgcgatcg ccgagtccga caaagagact gccgtctcga
      961 gcgaagacca gtctggatcc gaagtgcgaa ccatcctctc ttttgacggg ctgcctgaag
     1021 atcacacggg catcggcgac cctgtcgtcg atcagtcgac ctcgggccac cgccgtactc
     1081 cgcgcctgcg gcgacgaatc cgacgactcg gagaacgacc agtagatcgt ccggttcacc
     1141 gaaaagcccg ggtcgagtac gacgtcgagc aagccgccct cgtcgcctga ggccacgggc
     1201 aagagccccg ccaggggctc gctcaaggct ccattcgccg agacgatgcg catccgcccc
     1261 ggccgctccg tcacgagcat ccggccatcc ggcaggaagg cgatcgccca ggggtgttcc
     1321 agtcccttcg ccaccgtgac tggccgaagg ccctgcatgt cgagccttct gagaaccttg
     1381 ggcgtcaccc aggcggcgag ggccagtgcc acgatggcag ccaacagcag gatccatcct
     1441 ttgcgcttga gcatcatcga ttgccactcc gtgcgggcgc ggattctgcc agacaggccg
     1501 accccaatcg ccggagcgac gtgggggttc gttcgtgggg ggcgggcaaa agtgtgtact
     1561 tccgcacgct gaatcacctg ctgaagcacg gcgccatgac agtacgactg tgggggagcc
     1621 ccccgcatgg agggttgcgg ctgacatggc gccaaacata attcgtcaca agttgaaggg
     1681 tgccaaggaa gtagcaggtg gccctcgcga tccgattctg gctggtcgtc ttagcggagt
     1741 ctggctgacg caccagtttc acattgcgac cacgcggcat tcgctgctgc ggcacaggag
     1801 acagaatgtt ccgcgccttt caccatcgtg catacgctgc agtccttctc gacgccggga
     1861 tggacgcctt gctggggttc ctcgccgtgc tgatcgcggc ttggacaatc agtgcggtgc
     1921 cgatgagcga attcggccag acgctgtcca acccgtcggt gatgcttgct gcctcgggtt
     1981 tcgctgtctg catggcgatg ctccagtcgt tcctgggtct gtatcgccaa gcggggctgt
     2041 ccttcgcgtc ccagctcgca cgattgctcg tggcgatgag catcggtggc tacttgacct
     2101 atctggttct gaaggaggtc ggtttcgatg gtcatccgag ccgtcttgtc gggtattccg
     2161 tcgcgaacct gatcttcgcg atcctcgtgg tccgcggcac gatcgtgctg gtgcgcgcgg
     2221 cccaggggtc cagccgcgtg ctgatcgtgg gcaccggccc cgaagctgtt tccgtggcga
     2281 acgacctgcg tcgcagctcg cgcgtgagcg gcgaggtgat cggcttctac ccgaccccgg
     2341 ccgtggcgcc tgatcagaac atcgacggca agctgctgct tgacgcgcga gtgccgctgg
     2401 aacaactggc gcgcgagctc gagatcgact cgatcatcgt cgccacacgc gaacaacgcg
     2461 gtggcgcgat gccgatggat caactggtga tttgccgctc catgggcatt ccggtcatgg
     2521 atctcgctgg cttctacgag cgcacgcacg ccgaggtccc gctggacagc ctgaaggcca
     2581 gttggctcgt ctacgggccg ggcttcgtgc aggggcgtgt tcgccaggcg ctcaagcgcg
     2641 gtttcgacat cgtgacctcg tcgattctgc tcgtgctcgc cttgccggtg atgatcattg
     2701 ctgccgtggc gatcaagctc gacagccgcg ggcccctgat ctacaggcag gagcgagtgg
     2761 ggctgggtgg tcgcagtttc atgtgcctga agttccgcag catgtgtacg gacgcggagc
     2821 gcgatggcgt ggcgcgctgg gccacaaaga atgatgcccg cgtgacgcgt gtcgggcgca
     2881 tcatccgcaa ggcgcggatt gatga
//
LOCUS       DAOORV010000305         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_41775_length_2880_cov_11.961770, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000305 DAOORV010000000
VERSION     DAOORV010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41775_length_2880_cov_11.961770"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PKV56_19570"
     CDS             <1..1274
                     /locus_tag="PKV56_19570"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOX70046.1"
                     /translation="ARERGKRIKDHVIEHFDHYLLEFERNAIASGAQVHWASTADEAS
                     RIVVDLCRKANAKRVTRVKSMLGEEIGLPHALDAAGIERVETDLAEHIVQLGGDRPSH
                     IVWPSMHRTREQVSEMFKQSHRVPHREETIEAMVDSARRELRDKFLSADVSISGANFL
                     IADTGATCTVTNEGNAELTTSPPRVHIVTAGIEKLVPSLPHAFALLRLLVRSATGADV
                     TQYTTFHCGPKKPGDLDGPEEFHIVLVDNGRTKMLAEAGLRDMLRCLRCGACMNHCVV
                     FRQMGGHAYGGTYPGPMGAVLTPVFDGLEKSRDLPHACTLNGKCQEVCPVDIPLPTLL
                     RGWRDRSWREGLEPAAMRFGMGIFTFVASRPWLYRLGTAVQVRVLGLFSRGGWIRSMP
                     GLGAWTEHRDFPAPEGRTFMARYASGEGRRK"
     gene            1271..1918
                     /locus_tag="PKV56_19575"
     CDS             1271..1918
                     /locus_tag="PKV56_19575"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOX70047.1"
                     /translation="MTARDDILGRVRAALGRRSIDPVTVQGAAAALLADLPSTRPALL
                     APALVDAFIARVTSPKVAASAEQVASLAAVPAAVAAYLEHRRLPAVVALQPHALLRAL
                     DWSAFEVHARVAPDETAAVGMAKWGIAETGSLVFHSGADSPVLANFLPLHHIVAVRAA
                     DILAYLEDYAAAAAGQPMPRNVNLITGASGTTDIEGSLVLGAHGPRYMHVVVVGS"
     gene            complement(1920..2051)
                     /locus_tag="PKV56_19580"
                     /pseudo
     CDS             complement(1920..2051)
                     /locus_tag="PKV56_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(2092..2562)
                     /locus_tag="PKV56_19585"
                     /pseudo
     CDS             complement(2092..2562)
                     /locus_tag="PKV56_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
BASE COUNT          461 a          950 c          994 g          475 t
ORIGIN      
        1 ccgcgcgcga acgcggcaag cggatcaagg accacgtcat cgagcacttc gaccactacc
       61 tgctcgaatt cgaacgcaac gccatcgcct ccggcgccca ggtgcactgg gcgtcgacgg
      121 ccgacgaggc ctcgcgcatc gtcgtcgacc tgtgccgcaa ggccaacgcg aagcgggtga
      181 cgcgcgtgaa gtcgatgctc ggcgaggaga tcggcctgcc gcatgcactg gacgcggccg
      241 gcatcgagcg cgtcgaaacc gatctggccg agcacatcgt gcaacttggg ggcgatcggc
      301 cgtcgcacat cgtctggccg tcgatgcatc gcacgcgcga gcaggtgtcg gagatgttca
      361 agcaatctca ccgcgtcccg caccgcgagg agacgatcga ggcgatggtc gacagcgcgc
      421 ggcgcgagct gcgcgacaag ttcctgagcg ccgacgtgtc gatctcgggc gccaacttcc
      481 tgatcgccga taccggcgcg acctgcaccg tgaccaacga gggcaacgcc gagctgacga
      541 cctcgccgcc gcgtgtgcac atcgtgacgg ccggcatcga gaagctggtg ccgagcctgc
      601 cgcacgcgtt tgcgctgctg cgcctgctgg tccgctcggc caccggagcc gatgtcacgc
      661 agtacaccac cttccattgc gggccgaaga agccaggcga tctggacggc cccgaggagt
      721 tccacatcgt gctggtcgac aacggccgca cgaagatgct cgccgaggct ggcttgcgcg
      781 acatgctgcg ctgcctgcgc tgcggcgcgt gcatgaacca ctgcgtggtg ttccggcaga
      841 tgggcgggca tgcctatggc ggcacctatc ccgggccgat gggggccgtg ctcacgccgg
      901 tcttcgacgg cctggagaag tcccgcgatc tgccgcatgc gtgcacgctc aacggcaaat
      961 gccaggaggt ctgcccggtc gacattccgc tgccgacgct gctgcgcggc tggcgcgacc
     1021 gctcgtggcg cgaagggctg gaaccggcgg cgatgcgctt cgggatgggc atcttcacgt
     1081 tcgtagcctc gcgcccatgg ctgtaccggc tgggcacggc ggtgcaggtg cgtgttctgg
     1141 ggctgttcag ccgcggcggc tggatacgca gcatgcccgg gctcggcgcg tggacggagc
     1201 accgcgactt tccggcgccc gagggccgca ccttcatggc ccgctacgcc agcggcgagg
     1261 ggaggcgcaa gtgacggcgc gcgacgacat cctgggccgc gtgcgcgctg cgctcgggcg
     1321 gcgcagcatc gatccggtca ccgtgcaggg cgccgccgcg gcgctgctgg cggacctgcc
     1381 gtcgacgcgc cccgcgctgc tcgccccagc gctggtggac gccttcatcg cgcgggtcac
     1441 ttcgccgaag gttgctgcct cggcagaaca ggtcgcgtcg ctggctgcgg tgcctgccgc
     1501 ggtggccgcg tacctggagc atcgcaggct gccggccgtg gtcgcgctgc agccgcacgc
     1561 gctgctgcgc gcgctcgact ggtcggcctt cgaggttcat gcccgcgtcg cgccggacga
     1621 aactgctgcg gtgggcatgg cgaagtgggg catcgcggag accggatcgc tggtgttcca
     1681 ttccggcgcc gactcccccg tgctggcaaa ctttctgccg ctgcatcaca tcgttgcggt
     1741 gcgcgctgcc gacatcctgg cctatctgga ggactatgca gcggcagctg cgggccagcc
     1801 gatgccgcgc aatgtgaacc tgatcaccgg ggcgagcggc accaccgaca tcgaaggcag
     1861 cctggtgctg ggggcacacg ggccgcgcta catgcatgtg gtcgttgtgg gctcgtaacg
     1921 tcggccagga acgccgtgcg cagcagtttg cccagctgca cgccggcgtc gtagatcggg
     1981 tcacctcttg cggcagagcc gaaccgggcc agcgccgtca ccgcgctggc gtgcccgctc
     2041 gataccgatg cggtgtgaga gcgagccgct ggcaatgatc gccaccttgc caggataggc
     2101 atcgatggcc tcgcgaaacg cagcgccgac acgtcggctt tcgtcgatcg tcgagtaggc
     2161 acaccatccc gaaacggaca cgaccttcac cttgcggcgc aggttcatga agtgcatcgg
     2221 caccagggtg ccgtactcga gcggcaacag gcggctgcgg tgcgcgatgg tcgtgacccc
     2281 acgctccgag gctgcttccg ccagagcgac ggccagttcg ggattgccgg catggtcgaa
     2341 ttccagcgac tcgatctgcg tcgggaactc ggtgctctgg aacacgccct ggaaggtgtc
     2401 gtgagcgttg acgtggtacg cggcattgct gagccaatgg gtgtccagga tcaccaccgt
     2461 gtcggcgccg gcttgcacga tgcggtcacc gatcagccgc aggccgtcga tcgcgtcctg
     2521 gcggcagccg tgcagcggac caggcaactc tgacagcacc atccggggaa tgtgggtgac
     2581 cttggcggcc aggacgaggc tcattcgtgt ctccgtgtgg gttgaagctg ttgagatccg
     2641 cgaaccctgc tagccatgtc catgaaccgt gctagcgaga cttgcggcta aggtattgat
     2701 tcatcaagga aattcgggtt ctgaacccct ctcgatgtct gttgcgagct gaccacaaca
     2761 cacgacaccg agtccacgaa ccccgctatt gcatctggcg tgtccatgaa gcatgctact
     2821 ggcgggtttg tgaggtgaac ggatgcagtc gccccgtgag atcggcgaac gtgaagtcca
//
LOCUS       DAOORV010000306         2837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_42625_length_2837_cov_4.648454, whole genome shotgun sequence.
ACCESSION   DAOORV010000306 DAOORV010000000
VERSION     DAOORV010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42625_length_2837_cov_4.648454"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKV56_19590"
     CDS             complement(<1..468)
                     /locus_tag="PKV56_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOX70048.1"
                     /translation="MTTLRQADLVESIAAALQYISYYHPADYIAHLARAYESEQSPAA
                     KDAIAQILTNSKMCAEGRRPICQDTGIVNVFLKIGMDVRFEGFTGSIEDAVNEGVRRG
                     YMNPDNTLRASIVADPQFERKNTKDNTPAVVHMKLVPGNTVDVQVAAKGGGSEN"
     gene            complement(544..1185)
                     /locus_tag="PKV56_19595"
     CDS             complement(544..1185)
                     /locus_tag="PKV56_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HOX70049.1"
                     /translation="MSLPTPQSPSLKLRPLPALIFASRWLQLPLYLGLILAQGVYVFH
                     FWVELLHLIEAVFGSEKALSALVQSIGYQLPPIKDAAGMVIGYEAPKLNETVIMLVVL
                     ALIDVVMISNLLIMVIVGGYETFVSRMGLEEHPDQPEWLSHVNASVLKVKLATAIIGI
                     SSIHLLKTFISAGNYSEKVLMWQTLIHITFLLSAIAIAATDRLMAHAAPPKNH"
     gene            1430..>2837
                     /locus_tag="PKV56_19600"
     CDS             1430..>2837
                     /locus_tag="PKV56_19600"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOX70050.1"
                     /translation="MSDSTSVKQYLPPADLARDAHVSGMASYQALCAEAEADYAGYWA
                     RLAREMVAWKTPFTRTLDESNAPFFKWFEDGTLNASYNCLDRQVEAGLGDKVAIIFEA
                     DGGQVTKVTYSELLSRTCQLANALKSRGIKKGDRVVIYMSMSVEGVVAMQACARIGAT
                     HSVVFGGFSAQSLQDRIKDTGAVLVITADEQLRGGKALPLKAIVDDALASGQCDSVKD
                     VIVYRRTGGKIAWNPRDLWLHEVMEAQPATCEPEWVGAEHPLFLLYTSGSTGKPKGVQ
                     HSTGGYLLHAMLTTKWTFDLKPNDVFWCTADIGWVTGHTYIAYGPLALGATEIVFEGV
                     PTYPDAGRFWKMIQDHKVSIFYTAPTAIRSLIKAAESNEAVHPRKYDLSSLRILGSVG
                     EPINPAAWEWYHEHIGGSRCPIVDTFWQTETGGHMITPLPGVTTLVPGSCTLPFPGIM
                     AAVVDETGKDVPWGQGGIL"
BASE COUNT          508 a          850 c          944 g          535 t
ORIGIN      
        1 gttttcgctg ccgccgcctt tggctgcgac ctgcacatcg acggtgttgc ccggcacgag
       61 cttcatgtgg acgacggcgg gcgtgttgtc cttcgtgttc ttgcgctcga attgcgggtc
      121 cgccacgatg ctggcccgca acgtgttgtc gggattcatg taaccgcggc gcacgccttc
      181 gttgacggcg tcctcgatgc tgcctgtgaa tccttcgaag cgcacgtcca tgccgatctt
      241 caggaagacg ttgacgatgc ctgtgtcttg acagatcggc cggcggcctt cggcgcacat
      301 cttcgaattg gtcaggatct gcgcgattgc gtccttcgcc gccgggcttt gctcgctttc
      361 ataggcgcga gccaggtggg cgatgtagtc ggccgggtgg tagtagctga tgtactgcag
      421 cgcggcggcg atcgattcga cgaggtcggc ttggcgaagg gttgtcatga tctgggttgc
      481 tcgagcgaga gagatggatc ggtgcgcggg gcggcgccgg gccggccccg tcggactgtg
      541 gcctcagtgg ttctttggcg gtgccgcatg cgccatcagg cggtcggtcg cggcgatggc
      601 gatggccgaa agcaggaagg tgatgtgaat cagcgtctgc cacatcagca ccttctcgga
      661 atagttgccg gcgctgatga atgtcttgag caggtggatc gagctgatgc cgatgatcgc
      721 ggtggcgagt ttcaccttca gcaccgacgc attgacgtgg ctgagccatt caggctgatc
      781 cgggtgctct tccagcccca tgcgcgagac gaaggtctcg tagccaccca cgatgaccat
      841 gatcagcagg ttggagatca tcaccacgtc gatcagtgca agcacgacca gcatgatcac
      901 cgtctcgttg agcttcggtg cctcgtagcc gatcaccatg ccggccgcgt ccttgatcgg
      961 cggcaactgg tagccgatgc tctggaccag ggcggagagt gccttttcgc tgccgaacac
     1021 cgcttcgatc aaatgcagca gttcgaccca gaaatggaaa acatagacgc cctgtgccaa
     1081 gatcaaacca aggtacagcg gcaactgcag ccagcggctc gcaaaaatca gcgcgggtag
     1141 cggacgcagc ttcagggatg gggactgcgg agtcggaagg ctcatggcgg gtgtggggct
     1201 ctgcggtcag tgatctcggc gcttccggtg tcggattgac ccggtcggcg gccgattcgc
     1261 gccagattgt aggcttcggg tcttgaccgg cttgcccgtt tcggtgtcag agtaaggggt
     1321 tcgtaagagg gcgtcgctat ggtcatggcc cattgattgc agccgcaagg gctgatgtgt
     1381 agcagtcgat cgggaactgc tccagaacag atcaaaacag gagacaacga tgagtgattc
     1441 cacttccgtg aagcagtacc ttccccctgc cgacctggcg cgcgacgccc atgtttccgg
     1501 catggccagc taccaggccc tgtgtgccga agccgaggcc gactatgccg gttactgggc
     1561 gcgcctcgct cgcgagatgg tggcgtggaa gacgccgttc acccgcacgc ttgacgaaag
     1621 caacgcgccg ttcttcaagt ggttcgagga cggcacgctg aacgcctcgt acaactgcct
     1681 cgaccggcag gtcgaggccg gtctcggcga caaggtggcg atcatcttcg aggccgatgg
     1741 cggtcaggtc accaaggtca cctacagcga actgctgtcc cgcacctgcc agctggccaa
     1801 cgcgctgaag tcgcgtggca tcaagaaggg cgatcgcgtc gtcatctaca tgtcgatgag
     1861 cgtcgagggt gtggtcgcca tgcaggcctg tgcgcgcatc ggtgcgacgc actcggtggt
     1921 gttcggcggg ttctcggcgc aaagcctgca ggatcgcatc aaggacactg gcgccgtgct
     1981 ggtgatcacg gccgacgagc aattgcgcgg gggcaaggcg ctaccgttga aggcgatcgt
     2041 cgacgatgca ctggcttcag ggcagtgcga cagcgtcaag gatgtgatcg tgtatcgccg
     2101 cacgggcggc aagatcgcct ggaacccgag agacctctgg ctccacgagg tgatggaggc
     2161 acaaccggcg acctgcgaac cggagtgggt cggcgcggaa cacccgctgt tcctgctgta
     2221 cacgtcgggt tcgaccggca agcccaaggg cgtgcagcat tcgaccggcg gctacctgct
     2281 gcacgcgatg ctgacgacga agtggacctt cgacctgaag ccgaacgatg tcttctggtg
     2341 cacagccgac atcggctggg tcaccgggca cacctacatc gcctacggac cgcttgcact
     2401 cggtgcgacc gagatcgtgt tcgagggcgt gccgacgtat cccgacgccg gacgcttctg
     2461 gaaaatgatc caggatcaca aggtcagcat cttctacacg gcgccgaccg caatccgctc
     2521 gctgatcaag gccgccgaat cgaacgaggc ggtccatccg cgcaagtacg acctgagcag
     2581 cctgcgcatc ctgggctctg tcggcgagcc gatcaaccct gctgcctggg agtggtacca
     2641 cgagcacatc ggcggcagcc gctgcccgat cgtcgacacg ttctggcaga ccgagaccgg
     2701 cggccacatg atcacgccgc tgccgggcgt caccacgctg gtgccgggct cctgcacgct
     2761 gccgttcccg ggcatcatgg ctgcggtggt cgacgagacc ggcaaggacg tgccctgggg
     2821 ccagggcggc atcctgg
//
LOCUS       DAOORV010000307         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_44239_length_2763_cov_5.120015, whole genome shotgun sequence.
ACCESSION   DAOORV010000307 DAOORV010000000
VERSION     DAOORV010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44239_length_2763_cov_5.120015"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PKV56_19605"
     CDS             complement(<1..167)
                     /locus_tag="PKV56_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX70051.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLIQRCGGLSEREMESQVLDSM
                     DIERERGITIKA"
     gene            complement(611..2116)
                     /locus_tag="PKV56_19610"
     CDS             complement(611..2116)
                     /locus_tag="PKV56_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOX70052.1"
                     /translation="MSPLYRAPLRKGWHAWLSGCVLAVVLALALAPVPARAQALPDFS
                     SLVESVGPAVVNIRTTEKARPSRGNGAPEMDEDMLEFFRRFGVPIPGQPQTPRRNPQQ
                     PPQQQPPQQQSPQQDSEPQQRGVGSGFILSADGYVMTNAHVVDGAEEVFVTLTDKREF
                     KARVIGADKRTDVALVKIEATSLPAVKLGDVSRLKVGEWVIAIGSPFGLENTVTAGIV
                     SAKARDTGDFLPFIQTDVAINPGNSGGPLINMRGEVIGINSQIYSRSGGFMGISFAIP
                     IDEATRVSDQLRSNGRVIRGRIGVQIDQVTKEVAESIGLGKPAGAMVRSVESGGPADK
                     AGVEAGDIITRFEGRVIEKAGDLPRIVGGVKPGTKATVQVFRRGAYKELAVTVVELEA
                     ERVGQAPDRGGAPKPPVAIGLLGLAVSDLSEAQKRELKVKGGVRVEGAEGAAARSGIR
                     EGDVVLSLDNTEITSAKQFEAVVSKLEKPKSVTALVKRGDWVNYIVIRPAR"
     gene            complement(2218..>2763)
                     /locus_tag="PKV56_19615"
     CDS             complement(2218..>2763)
                     /locus_tag="PKV56_19615"
                     /inference="COORDINATES: protein motif:HMM:NF028497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HOX70053.1"
                     /translation="AYRLWAERESGLLLRVEVLNALGEVLEVSAFSDVVIGVRAQPEI
                     VLQPMRKLAGYRVIKPALQATALDAEGWELRRTVAGFRQLSCVRRPLEVFHAPDVKTG
                     AAAASQPVQAVQTIFSDGITYVSIFIEPFNADRHSRPVITVVGATQTMMSRRGDHWVT
                     VVGDVPAVTLRAFTNALDRRK"
BASE COUNT          520 a          885 c          823 g          535 t
ORIGIN      
        1 gccttgatgg tgatgccgcg ctcgcgctcg atgtccatcg agtcgagcac ctgcgattcc
       61 atctcgcgct cgctcaaacc accgcagcgt tgaatcagcc gatcagccag cgttgacttg
      121 ccgtgatcga tgtgggcaat gatcgagaaa tttcggatgt gatccatgct tgctgcgtgt
      181 tcttcagtgg agcttcgatg aggttcccaa acggcgcagc cgtttcagcg aaagctcata
      241 tcgcgtagcg atgtgcgggg ccgcaggcct gtgctgaagc taaaaaaaag gcacgtcgaa
      301 cgattgacgc gccttccaac aaaaatgttt ggcaactgct tgttggggtt tcattgtagc
      361 caaaagcacg actgaagaac cgcgccaatg cttgtttggc gatcgttgcg ctccgccaac
      421 ggaaaatttg tacacacctt atcaacaggc tgacgaagct attttcgtgc ccatcgtttg
      481 aacttcagcg ggtgcgactc cgggcctcta tcgaatttca tcgatgcctg gcttccatca
      541 ggcaagccgt gctggaaagc agccttgagc atcgtatgtg agcattcgca aactttagtc
      601 ggcagcccgg tcagcgtgcc ggacggatca cgatgtagtt cacccaatcg ccacgcttga
      661 ccaatgccgt gaccgatttc ggcttctcca gcttgctcac gacggcctcg aactgcttcg
      721 cactggtgat ttcggtgttg tccagagaca gcacgacatc cccttcgcgg ataccggacc
      781 gagcggcagc cccctcggcc ccctccaccc gcacgccgcc cttgaccttg agttcacgtt
      841 tttgcgcctc gctcaggtcc gacacagcca gaccaagaag accgatcgcc accggcggtt
      901 tgggagcgcc gccgcgatcg ggggcctggc cgacacgctc cgcctcgagt tcgaccacgg
      961 tgacagccag ttccttgtaa gcgcctcgac ggaaaacctg cacggtggcc ttcgtgccag
     1021 gcttcacgcc gcccacgatg cgcggcaggt caccggcctt ctcgatcaca cgcccctcga
     1081 accgggtgat gatgtcgccc gcctcgacgc cggccttgtc cgcaggaccg cctgactcga
     1141 cgctgcgaac catggcgcca gcgggcttgc ccaagccgat ggattcagcc acttccttgg
     1201 tgacctgatc gatctgcaca ccaatgcgac cgcgaatcac ccggccgttg ctgcgcaact
     1261 ggtcggacac acgggtcgcc tcgtcgatgg gaatggcgaa cgagataccc atgaaaccgc
     1321 ctgatctgct gtagatctgc gagttgatcc cgatcacctc gccgcgcatg ttgatcagcg
     1381 gaccgccgga gttgccgggg ttgatcgcca cgtcggtctg gatgaagggc aggaaatcgc
     1441 cggtgtcgcg cgccttggcg ctgacgattc cggcggtgac ggtgttctcc agcccgaacg
     1501 gggagccaat ggcaatgacc cattcaccga ccttgagtcg gctcacatca cccagcttca
     1561 cggctggcag gctcgtggcc tcgatcttca ccagggcaac gtcggttcgc ttgtcggcac
     1621 cgatgacgcg tgccttgaat tcgcgcttgt cggtgagcgt gacgaacact tcctcggcgc
     1681 catcgaccac gtgcgcattg gtcatgacgt aaccgtcggc actgaggatg aaccccgagc
     1741 ccacgccgcg ctgctgaggc tcgctgtctt gctgaggcga ctgctgttgt ggcggttgtt
     1801 gctgcggcgg ctgctgagga ttgcgcctgg gagtctgtgg ctgcccagga atcggcacgc
     1861 cgaatcgacg gaagaactcg agcatgtctt cgtccatctc cggagccccg tttccacgcg
     1921 atggacgcgc cttttccgtc gtgcggatgt tgaccacggc gggtccgacg gattcaacca
     1981 ggctggaaaa atcgggcagg gcctgggcac gcgccgggac gggcgcgagg gccagtgcga
     2041 ggaccacggc aagcacgcaa ccactcaacc atgcatgcca tccctttcgc aggggcgccc
     2101 tgtagagcgg gctcatcagt ctggtcgttt gcagtggcgt ttgcatgatc gggactcttg
     2161 cgatggaaaa atggaaaaga gatccgccgg cctggcggcg acgtaggaaa agatgcgcta
     2221 cttcctgcga tcgagtgcgt tcgtgaacgc gcgcagcgtc acggcgggca cgtcgccgac
     2281 caccgtcacc cagtggtcgc cgcgccgact catcatggtc tgcgtcgctc cgaccacggt
     2341 gatcaccggg cgggaatgac ggtccgcgtt gaacggctcg atgaagatgg agacgtacgt
     2401 gatcccgtcg gaaaagatcg tctgaacggc ctgaaccggc tgcgacgctg cggccgcgcc
     2461 ggtcttcacg tcaggcgcgt gaaacacttc aagcggtcgc cgcacgcaac tcagttgcct
     2521 gaatcctgca accgtccggc gcaactccca accttccgca tccagggcgg tggcctgcaa
     2581 agccggcttg atcactcggt aaccggcgag cttgcgcatg ggctgcagca cgatctctgg
     2641 ctgtgcacgc acgccaatga ccacgtccga gaacgcagac acctcgagca cctcgcccag
     2701 ggcattcagt acttcgaccc gcagcaagag cccgctttcc cgctcagccc acagtcggta
     2761 ggc
//
LOCUS       DAOORV010000308         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_44326_length_2759_cov_9.669749, whole genome shotgun sequence.
ACCESSION   DAOORV010000308 DAOORV010000000
VERSION     DAOORV010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44326_length_2759_cov_9.669749"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2620
                     /locus_tag="PKV56_19620"
     CDS             <1..2620
                     /locus_tag="PKV56_19620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOX70054.1"
                     /translation="HQPVDALLHTADERLFIPSREEAGQEDEAVAGRPQVKDVPLNPV
                     TTRGQDPELYWLHKYGADDADQRLRIDIRSLYRHEHIEPERLIARLYALKNRDAAQNE
                     LFTNELHGNPLAIDELDKPASYYKRHDTWRNRLIQGDSLLVMTSLLEREGMAGAVQCI
                     YIDPPYGIKYGSNWQMRVNDRNVKDGADEHLSGEPEVIKAFRDTWELGIHSYLSYLRD
                     RLLVAKELLSNSGSCFVQISDENVHLVRCLMDEVFGSENFVAQITFTKTSSATVTLLP
                     GTADYIIWYARDKASCKYRTLYREKKFGDAGADKYDQVELPDGTRRAMTRDEKLNPDS
                     LPASARIYRLDNLLSQAEGREKGEGAACWFPVEMNGREFLPNERSRWKTNEIGMDRLQ
                     GSRRIQASTNSLGYVRFLDDFPAFALSNVWTDIGGIQSRLDKKVYNVQTSTTAIQRCI
                     LMTTDPGDLVLDPTCGSGTTAHVAEQWGRRWITSDTSRIALNIAKTRLMTATYPWYEL
                     ADKAGGDIRHGFDYKKVPHVTLKSIANGEPPDTETLYDQPKEDKKKLRVAGPFTVETL
                     QGFEPLAPEGVATANTDPVDSQRFADRVFEHLKHAGIKNGRKNEMAVFNRVDAINEQM
                     GDGGALHAEGWYADADGAERKAYFHIGPQFGTVSKKAVNDAILACRRRADGDWLVILG
                     FGFESDVDNRSVTLKVGSFEVTKVRMHDDLMQDGLIKKDKKAASFVTIGEPDIATVPA
                     TLADGSPGVAVEIRGLDLYDPMTDEVKERSAADISYWMVDERYDGANFVATQVFFCGG
                     DKDEFDKWKRGLSALAVASAKAKAEKTLKLEIDDEAFERVYGLQSHPIRAQRGQRVAV
                     RVISQFGEECTKVLVL"
BASE COUNT          592 a          865 c          890 g          412 t
ORIGIN      
        1 gcaccagccg gtggatgcgc tgctgcacac cgctgacgag cgcctgttca tccccagccg
       61 tgaagaggcc gggcaggaag acgaggctgt ggccggccgg ccgcaggtga aagacgtgcc
      121 gctgaacccc gtcaccaccc gcgggcaaga ccccgagctg tactggctgc acaagtacgg
      181 cgccgatgac gctgaccagc gtctgcgcat cgacatccgc agcctgtacc ggcatgagca
      241 catcgagccc gagcgcctga tcgcccgact gtatgcgctg aagaaccgtg atgccgcgca
      301 gaacgagctg ttcaccaatg aactgcacgg caacccgctg gccatcgacg aactcgacaa
      361 gcccgccagc tactacaagc ggcatgacac ctggcgcaac cgcctgatcc agggcgacag
      421 cctgctggtg atgaccagcc tgctggagcg tgaaggcatg gccggtgcgg tgcagtgcat
      481 ctacatcgac ccgccgtatg gcatcaagta cggcagcaac tggcagatgc gggtgaatga
      541 ccgcaacgtg aaggacggcg ccgacgaaca tttgagcggc gagcccgagg tgatcaaggc
      601 atttcgcgac acctgggaac tgggcatcca ttcgtacctg agctacctgc gcgaccggct
      661 gctggtggcc aaggaactgc tgagcaacag cggctcgtgc ttcgtgcaga tcagcgacga
      721 gaacgtgcac ctggtgcggt gcttgatgga tgaggtgttt gggagtgaga acttcgttgc
      781 tcagatcacc ttcacgaaga ccagcagtgc gacggtcacg ctgctgcctg gcacggccga
      841 ctacatcatt tggtacgcca gagacaaggc atcgtgcaag taccggacgc tgtatcgaga
      901 gaagaagttc ggcgatgcag gcgccgacaa gtacgaccaa gttgaactgc ccgatggaac
      961 tcgacgcgcg atgacgcgcg atgaaaaatt gaatccggac tcactgcccg ctagcgcccg
     1021 gatctatcgt ctcgacaacc tgctgagcca agcagaaggg cgcgaaaagg gcgaaggggc
     1081 tgcgtgctgg tttccagtgg aaatgaacgg acgagagttc ttgccgaacg agcgaagcag
     1141 gtggaaaacc aacgagattg gtatggaccg gctgcaaggc agtcgacgta ttcaggcttc
     1201 gactaactcc ctcgggtacg tgcgcttcct ggacgacttc ccggcgttcg cactgtccaa
     1261 cgtgtggact gacatcggtg gtatccagag ccgcctggac aagaaggtct acaacgttca
     1321 aacgtccacc acggccattc agcgctgcat cctgatgacc accgaccccg gcgatctcgt
     1381 gctcgacccc acctgcggca gcggcaccac cgcccatgtg gccgagcagt ggggccggcg
     1441 ctggatcacc agcgacacca gccgcattgc gctgaacatc gccaagacgc ggctgatgac
     1501 cgccacttat ccctggtacg aactggccga caaggccggc ggcgacatcc gccacggctt
     1561 cgactacaag aaagtgccgc acgtcacgct gaaaagcatc gccaacggcg agccgcccga
     1621 caccgagacg ctgtacgacc aacccaagga agacaagaag aagctgcgcg tggccggccc
     1681 cttcaccgtg gagacgctgc agggcttcga accgctggcg ccggaaggcg tggccaccgc
     1741 caacaccgac ccggtggaca gccagcgctt tgccgaccgg gtgtttgaac acctgaagca
     1801 tgccggcatc aagaacggcc gcaagaacga gatggcggtg ttcaaccggg tggacgcgat
     1861 caatgagcag atgggcgacg gcggcgcgct gcatgccgag ggttggtatg cagatgccga
     1921 cggcgccgag cgcaaggcct acttccacat cggcccgcag ttcggcaccg tcagcaagaa
     1981 ggcggtgaac gacgccatcc tggcctgccg ccgccgtgcc gatggcgact ggctggtgat
     2041 tctgggcttc ggcttcgaaa gcgacgtgga caaccgcagc gtgacgttga aggtgggcag
     2101 cttcgaggtg accaaggtgc gcatgcacga cgacctgatg caggacggcc tgatcaagaa
     2161 ggacaagaag gcggccagtt tcgtcaccat cggcgagccc gacatcgcca ccgtgccggc
     2221 cacgctggcc gacggcagcc caggcgtggc ggtggagatc cgcggcctgg acctgtacga
     2281 cccgatgacc gacgaggtga aggaacgcag cgctgctgac atcagctact ggatggtcga
     2341 cgagcgctac gacggcgcca acttcgtggc gacccaggtc ttcttctgcg gcggcgacaa
     2401 ggacgagttc gacaagtgga agcgcggcct gtcggccctg gccgtggcat cggccaaggc
     2461 caaggccgag aagaccttga agctggaaat cgacgacgag gcgtttgagc gggtgtacgg
     2521 cctgcaatcg caccccatcc gcgcgcagcg gggccagcgg gtggcggtgc gggtgatcag
     2581 ccagttcggc gaggaatgca ccaaggtgct ggtgctgtga ccgataccag ccgacgcacg
     2641 gcgacctgat tcagccagca acggacaatc gcacgcatgc gccctgcctt caccacccag
     2701 ctgtctgcgg cccagcgcca ggccgattcc atgctctgcg tcgggctcga ccccgagcc
//
LOCUS       DAOORV010000309         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_45102_length_2725_cov_7.716854, whole genome shotgun sequence.
ACCESSION   DAOORV010000309 DAOORV010000000
VERSION     DAOORV010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45102_length_2725_cov_7.716854"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKV56_19625"
     CDS             complement(<1..468)
                     /locus_tag="PKV56_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOX70055.1"
                     /translation="MTELLGIPIQALLGQLLLGLVNGSFYALLSLGLAVIFGLLGIVN
                     FAHGALYMIGAYVAWFALEKFGLGYWWALGLAPLIVGALGALIERTLLKRLYRLDPLY
                     GLLLTFGLALIFEGVFRDQFGVSGQQYPVPDALQGATDLGFMVLPNYRAWVIVA"
     gene            complement(599..1813)
                     /locus_tag="PKV56_19630"
     CDS             complement(599..1813)
                     /locus_tag="PKV56_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX70056.1"
                     /translation="MNLKKSVVACALATAALFTAATPARAQMSGDVIRIGFITDMSSV
                     YSDLDGPAGAEAIRMAVADLGGAIDGKKVEVLVADHQNKTDVAASKAREWFDQQGLDL
                     LICGTNSGACLAMAKVAQEKKKPFFVVGAGSAALTNEQCTPYTVHYAYDTVALAKGTG
                     GAVVKAGGKSWYFLTADYAFGQALQADTTTVVKAAGGTVAGSVKHPLNASDFSSFLLQ
                     AQSSKAQILGLANAGGDTVNAIKAANEFGVTKSMKMAGLLVFINDIHALGLKTTQGMY
                     LTDSWYWNQTPEARAWSRRFFEKFKRMPSSVQAGDYSAASHYLNAVKAAKTDDGDKVL
                     AQMRKTKINDFYAKNAYIREDGRMVHDMYLMQVKSPDQSKEPWDYYNVIATIPGDEAF
                     TTKADTKCSLWK"
     gene            complement(1870..2583)
                     /locus_tag="PKV56_19635"
     CDS             complement(1870..2583)
                     /locus_tag="PKV56_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX70057.1"
                     /translation="MTATAKRFIEVRGLQGWYGESHVLHGVDFHVDEGEVVTLLGRNG
                     AGRTSTLRAIMGLIGRRTGSIRIKGQETLDLPTHRIARLGIGYCPEERGIFSSLSAEE
                     NLLLPPVQAPGGMSIDEIYAMFPNLRERASSQGTRLSGGEQQMLAVARILRTGARLLL
                     LDEISEGLAPVIVQKLAEMVVALRRQGYTVLMVEQNFRFAAPLADRFLVMEHGRVIQQ
                     FTQAELPQRIGQLHEYLGV"
     gene            complement(2585..>2725)
                     /locus_tag="PKV56_19640"
     CDS             complement(2585..>2725)
                     /locus_tag="PKV56_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX70058.1"
                     /translation="ASIADTITVLQRGATLAEGPYAEVSKDPAVIEAYMGSASVELSG
                     AH"
BASE COUNT          459 a          929 c          877 g          460 t
ORIGIN      
        1 cgcgacgatc acccacgcgc ggtagttcgg cagcaccatg aagccgaggt cggtcgcgcc
       61 ctgcagtgcg tcgggcaccg gatactgctg gcccgacacg ccgaactggt cgcggaacac
      121 gccttcgaag atcagcgcca gaccgaacgt gagcagcagt ccgtacaacg gatcgagccg
      181 gtagagccgc ttgagcagcg tgcgttcgat cagcgcgccg agcgcaccga cgatcagcgg
      241 cgccagcccc agcgcccacc agtagccgag accgaacttc tcgagcgcga accaggccac
      301 gtaggcgccg atcatgtaca gcgccccatg cgcgaaattg acgatgccca gcagcccgaa
      361 gatcaccgcc agcccgaggc tcagcagcgc atagaacgag ccgttgacca gcccgagcag
      421 cagctgcccg agcagggcct ggatcgggat gcccaagagt tcagtcatca ggaagcgaga
      481 agcggcgcgt tgcgcacaag cgaagcgagg tgcccctcaa gccacgcctc ccggatccgg
      541 ctctgccggt ccgacaggcg gcgcccctcg ggggcaggag cgcagcgaat gggggccgtc
      601 atttccacaa cgaacacttg gtgtccgcct tggtcgtgaa cgcttcgtcg cccgggatcg
      661 tggcgatcac gttgtagtag tcccagggtt ccttcgactg gtccggcgac ttcacctgca
      721 tcaggtacat gtcgtgcacc atccggccat cctcgcggat gtaggcattc ttcgcgtaga
      781 agtcgttgat cttcgtcttg cgcatctgcg cgagcacctt gtcgccgtcg tcggtcttcg
      841 cggccttgac ggcattcagg taatgcgacg ccgccgagta gtcgcccgcc tgcaccgacg
      901 agggcatgcg cttgaacttc tcgaagaagc ggcggctcca ggcgcgcgcc tcgggcgtct
      961 ggttccagta ccagctgtcg gtgaggtaca tgccctgcgt ggtcttcagc cccagcgcgt
     1021 ggatgtcgtt gatgaacacc agcagccccg ccatcttcat cgacttggtg acgccgaact
     1081 cgttggccgc cttgatcgca ttgacggtat cgccgcccgc gttggccagc cccaggatct
     1141 gagccttgct cgactgcgcc tgcagcagga acgacgagaa atcgctggcg ttcagcgggt
     1201 gcttgaccga gccggccacg gtgccgccgg ccgccttcac gaccgtggtg gtgtcggcct
     1261 gcagcgcctg cccgaaggcg tagtcggcgg tcaggaagta ccacgacttg ccgccggcct
     1321 tcaccacggc gccgccggtg cccttggcca gtgcgacggt gtcgtaggcg tagtgcaccg
     1381 tgtacggcgt gcactgctcg ttggtcagcg cggcgctgcc ggcgccgacg acgaagaacg
     1441 gcttcttctt ctcctgcgcc accttggcca tcgcgagaca ggcgcccgag ttggtgccgc
     1501 agatcaacag gtccaggccc tgctggtcga accactcgcg cgccttcgac gcggccacgt
     1561 cggtcttgtt ctggtggtcg gccaccagca cctcgacctt cttgccatcg atcgcgccac
     1621 ccagatcggc gaccgccatc cggatcgcct cggcaccggc gggcccgtcg agatcggaat
     1681 agacactgga catgtcggtg atgaacccga tgcggatcac gtcgcccgac atctgggcac
     1741 gagccggcgt ggcagcggtg aaaagcgcgg cagtggccag cgcgcaggcg acgacggact
     1801 tcttgaggtt catgttgcga ctccggatat cgatcgggtt gtggtttgct gattcgctga
     1861 gagtcggcgt cagacgccga ggtattcatg caactggccg atgcgctgcg gcagttcggc
     1921 ctgggtgaac tgctggatca cgcggccgtg ctccatcacc aggaagcggt cggccagcgg
     1981 cgcggcgaag cggaaattct gttcgaccat caacacggtg tagccctgcc tgcgcaaggc
     2041 gaccaccatc tcggcgagct tctgcacgat caccggcgcc aggccttcgg aaatctcatc
     2101 gagcaaaagc agccgcgcgc cggtgcgcag gatgcgcgcc accgccagca tctgctgctc
     2161 gccgcccgac agccgcgtgc cctggctcga cgcacgctcg cgcaggttcg ggaacatcgc
     2221 gtagatctcg tcgatgctca tgccgcccgg cgcctgcacc ggcggcagca gcaggttctc
     2281 ttcggccgac aggctggaga agatgccgcg ctcttcgggg cagtagccga tgcccagccg
     2341 cgcaatgcgg tgcgtcggca ggtcgagcgt ctcctggccc ttgatcctga tcgagccggt
     2401 acgccgtccg atcaagccca tgatcgcgcg cagcgtgctg gtgcggccag cgccgttgcg
     2461 accgagcagc gtgacgacct cgccctcgtc gacgtggaaa tcgacgccat gcagcacatg
     2521 cgactcgccg taccagccct gcaggccgcg cacttcgatg aatcgcttgg cggtcgcagt
     2581 catgtcaatg cgcgccgctc agctcgaccg acgcgctgcc catgtaggcc tcgatcaccg
     2641 ccggatcctt cgacacctcg gcatacggtc cttcggccag cgtcgcgccg cgctgcagca
     2701 cggtgatggt gtcggcgatg ctggc
//
LOCUS       DAOORV010000310         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_45103_length_2725_cov_6.342322, whole genome shotgun sequence.
ACCESSION   DAOORV010000310 DAOORV010000000
VERSION     DAOORV010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45103_length_2725_cov_6.342322"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PKV56_19645"
     CDS             complement(<1..1572)
                     /locus_tag="PKV56_19645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOX70059.1"
                     /translation="MVTRPPARSTSSSPSAPADNYGESSIRVLKGLEPVKQRPGMYTR
                     TDNPLHIVQEVIDNAADEALAGHGKRIDVTLHTDGSVSVQDDGRGIPFGMHPEEQRPV
                     VEIVFTQLHAGGKFDKGSGGAYSFSGGLHGVGVSVTNALSKRLEVTVYREGQVAQLAF
                     AGGDVSEPLTLRKVLMSASSGDRRSGTTVRAWPDPKYFESVDLPKSDLTHLLRSKAVL
                     MPGAGVSLTTEKTGEVQTWLYQGGLRDYLMQTLTADPLIPLFEGEHHADGSESENFAK
                     GEGATWCVAFTEDGSPVRESYVNLIPTVNGGTHESGLKDGLFGAIKNFIDLHSLLPKG
                     VKLMPDDVFSRASFVLSAKVLDPQFQGQIKERLNSRDALRLVSSCVKPPLELWLNQHV
                     EYGKKLAELVIKQAQTRQRASQKVEKRKGSGVAVLPGKLTDCESRDLLHNELFLVEGD
                     SAGGSAKMGRNKETQAILPLRGKVLNSWEVERDRLFANNEIHDIAVAIGVDPHSATDT
                     PDLSGLRYGKVCILSD"
     gene            1705..2298
                     /locus_tag="PKV56_19650"
     CDS             1705..2298
                     /locus_tag="PKV56_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX70060.1"
                     /translation="MNEPSTASPHARAAHCGIPVSDWVRRWSHLVPARGRVLDLACGS
                     GRHTRWFHARGCVVTAVDRDAAALLGLQGVARICIADIEAGPWPLPCERFDAVVVTNY
                     LWRPLLRQVAESLADPGVLIYETFATGNASVGKPSNPDFLLRPGELLQFAAAHGLRVV
                     AYEDGFERDPDRYIQRIVAMRPGTDDTELHRHPLRCD"
     gene            2344..>2725
                     /locus_tag="PKV56_19655"
     CDS             2344..>2725
                     /locus_tag="PKV56_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOX70061.1"
                     /translation="MKPITGSIVALITPMHEDGSVDYDTLRSLMDWHIAEGTDCIGVV
                     GTTGESPTVSVEEHCEIIRVAVEHARGRVPVMAGAGGNSTREAIELTRYAQKVGADCS
                     LSVVPYYNKPSQEGIYQHFKAIAEA"
BASE COUNT          443 a          911 c          897 g          474 t
ORIGIN      
        1 gtcgctcagg atgcagacct tgccgtagcg caggccgctc aggtcgggcg tgtcggtggc
       61 cgagtgcgga tcgacgccga tcgccacggc gatgtcgtgg atctcgttgt tggcaaacag
      121 gcgatcgcgc tcgacctccc acgaattgag caccttgccg cgcaacggaa ggatcgcctg
      181 cgtctccttg ttgcggccca tcttggcgct gccaccggcc gagtcgccct cgaccaggaa
      241 cagctcgttg tgcagcaggt cgcggctttc gcagtcggtc agcttgccgg gcagcacggc
      301 cacgcccgag cccttgcgct tctcgacctt ctggctcgcg cgctggcgcg tctgcgcctg
      361 cttgatcacc agttcggcga gcttcttgcc gtattcgaca tgctggttca gccacagctc
      421 gagcggcggc ttgacgcaac tcgacaccag ccgcagcgcg tcgcggctgt tcagccgctc
      481 cttgatctgg ccctggaact gtggatcgag caccttggcg ctgagcacga agctggcacg
      541 cgaaaacacg tcgtcgggca tcagcttgac gcccttgggc agcaggctgt gcaggtcgat
      601 gaagttcttg atcgcgccga acaggccgtc cttcaagccc gactcgtgcg taccgccgtt
      661 gaccgtgggg atcaggttga cgtagctctc gcgcaccgga gatccgtcct cggtgaacgc
      721 gacgcaccag gtagcaccct cgcccttcgc gaaattctcc gactcggagc catcggcgtg
      781 gtgctcgccc tcgaacagcg ggatcagcgg atcggccgtc agcgtctgca tcaggtagtc
      841 gcgcagaccg ccctggtaca gccaggtctg cacctcgcct gtcttctcgg tcgtcagcga
      901 cacaccggcg cccggcatca gcaccgcctt gctgcgcagc aggtgcgtca ggtcggactt
      961 cggcaggtcg acgctctcga agtactttgg atcgggccag gcgcgcaccg tggtgccgct
     1021 cctgcgatcc cccgaagacg ctgacatcaa gaccttgcgc agcgtcagtg gctcgctcac
     1081 gtcgccgccg gcaaaagcca gctgcgcgac ctggccttcg cgatagacgg tgacctccag
     1141 ccgcttcgac agcgcgttcg tcacgctgac gccgaccccg tgcaaaccgc ccgagaagct
     1201 gtaggcgccg cccgatccct tgtcgaactt gccgcccgca tgcagttggg tgaagacgat
     1261 ctcgacgacc gggcgctgtt cttccggatg catgccgaac ggaatgcccc ggccgtcgtc
     1321 ctgcacgctg accgaaccgt cggtgtgcag cgtgacgtcg atgcgcttgc cgtggcccgc
     1381 cagggcctcg tcggccgcgt tgtcgatgac ctcctgcacg atgtgcagcg ggttgtcggt
     1441 gcgggtgtac atgcccgggc gctgcttgac cggctcgagg cccttgagca cgcggatcga
     1501 ggattcgccg tagttgtctg cgggagctga agggctggat gacgtcgagc gtgcgggggg
     1561 gcgggtgacc atggcggcgc attgtaggga gcgggtgcgc gtcgatcgcg gcggcccgag
     1621 aggccatcca ctacagtggc gctgcctcgc tggtcgggcc cgcccgctgc gcccgaacac
     1681 cttccctgtc caaccgccac agccatgaac gaaccttcga ccgccagccc gcatgcccgc
     1741 gcagcgcact gcgggatccc cgtttcagac tgggttcgcc gctggtcgca tctggtgccg
     1801 gcgcgtggca gggtgctcga cctcgcctgc ggcagcgggc gccacacgcg ctggttccac
     1861 gcacgaggat gcgtcgtcac cgcggtggat cgcgacgcag ccgcactgct ggggttgcag
     1921 ggcgtcgcac gcatctgcat tgccgacatc gaagccggcc cgtggccgct gccgtgcgag
     1981 cgcttcgatg ccgtggtcgt caccaactac ctgtggcgac cgctgctgag gcaggtggca
     2041 gaaagcctgg ccgatccggg cgtgctgatc tacgaaacct tcgcgacagg gaacgccagc
     2101 gtcggcaaac cctccaaccc ggacttcctg ctgcgcccgg gagaactgct gcaattcgcc
     2161 gcagcgcacg ggctgcgcgt ggtggcttac gaagacgggt ttgagcgcga tcctgatcgg
     2221 tacatccagc gcatcgtcgc aatgcggccg gggaccgatg acaccgagtt gcatcgacat
     2281 ccgctgcgct gcgactgaag gtcgggtgtg cccgctaaaa tcaagggcta tcaaggaatt
     2341 tgcatgaaac cgatcactgg cagcatcgtc gcgctgatca cgccgatgca cgaggacggc
     2401 agcgtcgatt acgacacgct gcgcagcctg atggactggc acatcgccga gggcaccgat
     2461 tgcatcggag tcgtcggaac gaccggcgaa tctcccacgg tctcggtgga agagcactgc
     2521 gaaatcatcc gcgtggcggt cgagcatgcg cgcggacggg tgccggtgat ggccggagca
     2581 ggcggcaact cgacgcgtga agcgatcgag ctgacccgct acgcccagaa agtcggggcc
     2641 gattgctcgc tgtcggtcgt gccctactac aacaagcctt cgcaggaagg catctaccag
     2701 cacttcaagg cgatcgccga ggcgg
//
LOCUS       DAOORV010000311         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_45126_length_2724_cov_8.403522, whole genome shotgun sequence.
ACCESSION   DAOORV010000311 DAOORV010000000
VERSION     DAOORV010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45126_length_2724_cov_8.403522"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKV56_19660"
     CDS             <1..371
                     /locus_tag="PKV56_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX70062.1"
                     /translation="GVIMLLYLKQAWEARLARGQGGDADLLEAIREGAVLRVRPKAMT
                     VAVIIAGLVPIMFGGGTGSEVMQRIAAPMVGGMVTAPLLSMFVVPASYRLMRRRSRAN
                     AGAAGDPGNASALTGTPSAA"
     gene            759..1022
                     /locus_tag="PKV56_19665"
     CDS             759..1022
                     /locus_tag="PKV56_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70063.1"
                     /translation="MAGHELDADADAVVASADEVSVVVKASQAAKQKCSVCASCCSLG
                     AILTTVPVVPATDSAPTVFTTVVPTVGAFAADGPDRPPRYVVA"
     gene            1192..1521
                     /locus_tag="PKV56_19670"
     CDS             1192..1521
                     /locus_tag="PKV56_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70064.1"
                     /translation="MLLVAQAQAQSPTTNRPDPLDPKAQVPSVRYESSFAQFRRIGDD
                     KPLAWREANDAVARIGGWRVYAREAQQPDAAAAEKPSAPAQAPSQTPSTAPATKPMPA
                     GQPGHKH"
     gene            1532..>2724
                     /locus_tag="PKV56_19675"
     CDS             1532..>2724
                     /locus_tag="PKV56_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX70065.1"
                     /translation="MNHDIHRFSESRSGVRGLRVLALLTSAVVLGGCASFSPDGGFGT
                     VERTTKERIGKDLHWARGDDDLDTIAKRVAELLAKPLTVDEAVQVALLNNRGLQADFQ
                     ELGITEAEVVQAGRLPNPGFSFGRSSRGDEREIERGLHFNLARLVAMPLIGRMEARRF
                     EQVKGRVAMNVLSLAADTRKAYFTALAAEETVRYMRQVKQAADASAELARRMEQVGNF
                     NKLQRAREQSFYASAALQLARAEQVQRSTRERLVRLLGLWGAQTQFALPERLPDLPPS
                     ALDLPDVEQVALAQRLDVQGAKLAAEQTASNLGLTRTTRFVNVLELGVMRNSSNEAPT
                     QRGWEIGFELPLFDWGGARVARAEAIYMQTLHRAAETAINARSEVRESYTGYRSAYDI
                     ARHHR"
BASE COUNT          444 a          903 c          943 g          434 t
ORIGIN      
        1 tcggcgtgat catgctgctg tacctgaagc aggcctggga agcgcggctg gcgcgtgggc
       61 agggcggtga cgccgacctg ctcgaggcta tccgcgaggg tgcggtgctg cgcgtccgac
      121 ccaaggcgat gacggtcgcc gtgatcatcg cgggactggt gcccatcatg ttcgggggtg
      181 gcactggttc cgaggtgatg cagcgcatcg ccgcgccgat ggtgggcggc atggtcacgg
      241 cgccgctgct ctcgatgttc gtcgtgccgg cgagctaccg gctgatgcgc aggcgcagcc
      301 gagccaatgc tggcgcagcc ggcgatcctg ggaatgcctc ggccctgacc gggacgccat
      361 cagcagcctg agtgcaaccg gttgtggtcc gccgacgcgg ggagcgattg ctgcccaggg
      421 cccgaaacgt ccgcagcctc gaccgggggc atgaggcgtc gggtcaggca tgcgacctgc
      481 atggaagagt gcgcaagggt ggcggctcgg tcgaaattca cgttgcacaa aagggttccg
      541 gcgagccttg cctgggtcgc gaagaaggca tgcagtaaac tgtcaaaatg ggtttgcttg
      601 tcagaacgct tctgatttgg ttgttggtgt tggcagtgcc tgcgcaaggg gctgccgccg
      661 cgaccatggc gttctgcggc ccgaatcacc acggtggcgg atcgccccgc gccgaggtaa
      721 gtgctacgtc ttctgagcac tcccatcatg gcgatgccat ggccggtcac gagctcgatg
      781 cagatgcaga tgccgttgtg gccagtgccg atgaggtgtc cgtcgtcgtc aaggccagcc
      841 aagctgccaa gcagaagtgc agcgtctgcg cgtcctgctg ttcgctaggc gccatcctga
      901 ctaccgtgcc ggtcgttccg gccacggact cggcgccgac ggtgttcacc accgtcgtgc
      961 ccaccgtcgg cgcgttcgcc gccgacggcc ctgaccgccc ccctcgatac gtcgtcgcgt
     1021 aactggctcg ggttgacgcg cgtctagcga cgcgcggcct cgctccaggt tccgttccgt
     1081 ccccgccgcg acagcagccg ggacgtttcc agacgatcac gcgaggattt catggtccca
     1141 atcccaacgc gccggcgtat gccgtgcgct gtgtcgagct tgcttttcac gatgctgctg
     1201 gtcgcccaag cccaggcgca gtcgcccacc accaaccgtc ccgatccgct ggacccgaag
     1261 gcccaagtgc cctcggtgcg ctacgagtcg tcgttcgcac aattccgtcg catcggcgac
     1321 gacaaacccc tggcctggcg cgaggccaac gacgccgtcg cgcgcatcgg cggctggcgg
     1381 gtctacgcac gcgaagcgca gcaacccgac gctgctgcag ccgagaagcc atcggcaccc
     1441 gcgcaggctc cgtcccagac gccgtcgacc gcgccagcga ccaagccgat gcctgcgggt
     1501 cagcccggcc acaagcactg agggccgcac gatgaaccat gacattcaca ggttttccga
     1561 atcaaggagc ggcgttcgcg gcctccgggt gctggccctg ctgaccagcg ccgtcgtgct
     1621 ggggggctgc gcaagcttca gccctgatgg tggtttcggc acagtcgaac ggaccaccaa
     1681 agagcgcatc gggaaggacc tgcactgggc gcgcggcgac gacgacctcg acaccattgc
     1741 caagcgcgtg gcggagctgc tggccaagcc actgacggtg gacgaggcgg ttcaggtcgc
     1801 actgctcaac aaccgcgggc tgcaggccga tttccaggaa ctcggcatca ccgaggccga
     1861 ggtcgtgcag gccgggcgcc tgccgaaccc gggcttcagc ttcggccgct caagccgcgg
     1921 cgacgagcga gagatcgagc gcggtctgca cttcaacctg gcgcgcctgg tggccatgcc
     1981 gttgatcggc cgtatggagg cgcgccgctt cgagcaagtg aaggggcgcg tggcgatgaa
     2041 cgtgctgtcg ctggccgccg acacgcgcaa agcctatttc acggcgctgg cggcggaaga
     2101 gaccgttcga tacatgcggc aggtgaagca ggcggctgac gccagcgccg aactggcccg
     2161 gcggatggag caggtcggca acttcaacaa gctgcagcgt gcgcgggaac agtcgttcta
     2221 cgccagcgct gcgctccagc tggcccgggc agagcaggtc cagcgatcga cgcgcgagcg
     2281 cttggtgcgg ctgctgggct tgtggggcgc gcagacccag ttcgccttgc cagagcggct
     2341 gccggacctg ccgccttcgg cactggatct gcccgatgtc gagcaggtgg ccctggccca
     2401 gcggctggac gtgcagggcg ccaagctggc ggcggagcag accgctagca atcttgggct
     2461 gacacggacg acccgcttcg tcaacgtgct ggaactgggt gtgatgcgca acagctcgaa
     2521 cgaggcgccc acgcaacgcg ggtgggagat cggcttcgag ttgccgctgt tcgactgggg
     2581 aggcgcccgc gtcgcccgtg ccgaggccat ctacatgcag acgctgcacc gtgctgccga
     2641 gaccgcgatc aacgcgcgct cggaggtgcg cgagtcctac accggctacc gctcggccta
     2701 cgacatcgcc cgccaccacc gcga
//
LOCUS       DAOORV010000312         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_45333_length_2714_cov_6.445656, whole genome shotgun sequence.
ACCESSION   DAOORV010000312 DAOORV010000000
VERSION     DAOORV010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45333_length_2714_cov_6.445656"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PKV56_19680"
     CDS             complement(<1..949)
                     /locus_tag="PKV56_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOX70066.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKTIQAVGSEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     SVLRPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSIRVGGDKFDESIINYIRRNYGMLIGEPTAEA
                     IKKNIGSAFPGSEVKEIEVKGRNLSEGVPRSFTISSNEILEALTDPLNQMVSAVKNAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPV"
     gene            1245..1544
                     /gene="gatC"
                     /locus_tag="PKV56_19685"
     CDS             1245..1544
                     /gene="gatC"
                     /locus_tag="PKV56_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOX70067.1"
                     /translation="MALTPEDVGRIAHLARLELQPAEQAAMLTQLNAFFSIVERMSAV
                     DTGSVEPLYTPLSAVSEVSLRLRDDVVTESNQRELNQRSAPAVEDGLFLVPRVVE"
     gene            1541..>2714
                     /locus_tag="PKV56_19690"
     CDS             1541..>2714
                     /locus_tag="PKV56_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOX70068.1"
                     /translation="MSGALHELGVAELARALADKQVSSVEVTSHLLSRIDAHDHLGAW
                     LCVDAEAALTQAREADARRGRGSSPELLGVPLAHKDIFVTRELPTTAGSKMLEGYRSP
                     FDATVVSRLTGAGMVTLGKLNCDEFAMGSGNENSAYRAVRNPWDLTRVPGGSSGGSAA
                     AVAARLVPAATGTDTGGSIRQPASFSGITGIKPTYGVCSRFGMIAFASSLDQAGPMAR
                     SAEDCALLLGAMSGFDERDSTSAERPPQDYAAAMRKPRVGATATQPLTGLRVGLPKEF
                     FPAELTSGVDGVVRAALAELQRLGATLVDVSLPRTELSIPVYYIIAPAEASSNLSRFD
                     GVRFGHRAAKYTDLLDMYKKTRAEGFGDEVKRRIMIGSYVLSHGYYDAYYLQAQKLR"
BASE COUNT          462 a          902 c          890 g          460 t
ORIGIN      
        1 gcaccggcag cccggtttcc tcggcgagca ggcgatccag gtcgcgcagc agcgcacccc
       61 caccggtcag catcatgccg cgctcggcga tgtcggctcc cagttcggga ggcgtctgct
      121 ccagcgcgtt cttgaccgca gacaccatct ggttgagcgg atcggtcagc gcctcgagaa
      181 tctcgttgct gctgatcgtg aagctgcgcg gcacgccttc gctgaggttg cggcccttga
      241 cctcgatctc cttgacctcg gagcccggaa aggcgctgcc gatgttcttc ttgatcgcct
      301 cggcggtcgg ttcgccgatc agcatgccgt agttgcggcg gatgtaattg atgatcgatt
      361 cgtcgaactt gtcgccgccg acacggatgc tgcccttgta gaccatgccg cccaggctga
      421 tcacgccgac ctcggtcgta ccgccgccga tgtcaaccac catcgagccc gacgcttcgg
      481 acaccggcag gcccgcgccg atcgccgcag ccatcggttc ctcgatcagg tagacctcgc
      541 tggcgccggc gccgagcgcg gactcgcgga tggctcggcg ctcgacctgt gtcgagccgc
      601 agggcacgca gatgatgatg cgcgggctcg gtcgcagcac cgagcgcgga tgcaccatct
      661 tgatgaactg cttgagcatc tgctcggtca cggtgaagtc ggcgatcacg ccatccttca
      721 tcggccggat ggcttcgatg ttgccgggaa ccttgccgag catggccttg gcttccgagc
      781 cgacggcctg gatggtcttc ttgccgttgg gccccccctc gtggcgaatg gcgaccaccg
      841 agggctcatc gagcacgatg cccttgccgc gcacgtagat caacgtgttg gcggttccga
      901 ggtcgatggc gaggtccgtc gaaaagtagc gacgaaatga tccgaacata gtgtgtatgc
      961 ggtgatcagc gctggcgcgc acagcgaagg agcgcctcgc ttgaacccgt cgattccatg
     1021 gaatgaggga ggaggataaa gggcgaaacc agcgtcatcg ggacgcgacg cgcgaccgga
     1081 actgtccgca gcatcagcgg tttgcgaccc tcggttcgat caggcgacac gcccgctgaa
     1141 gacggcatgg gctgtgcgtc cgatgggttg acaaccaacg cagataatac ctgatggacc
     1201 cgggctcacg acccgccgca acccctcaac ggaccgaaat tcccatggct ttgacccccg
     1261 aagacgtagg ccgcatcgcc cacctggcgc ggctggagct gcagcctgcc gagcaggccg
     1321 ccatgctcac gcaactcaat gcgttcttct cgatcgtcga gcggatgagt gccgtcgaca
     1381 ccggcagtgt cgagccgctg tatacgccgc tgtcggccgt cagcgaggtc agccttcggt
     1441 tgcgcgacga cgttgtcacc gaaagtaacc agcgagagct caaccagcgc agcgcaccgg
     1501 cggtcgaaga cggcctgttc ctggtgccga gggtggtcga atgagcggcg ccctgcatga
     1561 actgggtgtg gccgaactgg caagagccct cgccgacaag caggtttcga gtgtcgaggt
     1621 cacctcgcac ctgctgtcgc gcatcgacgc tcacgaccac ctcggcgcct ggctgtgcgt
     1681 cgatgcggaa gctgcgctga cgcaggcccg cgaagccgat gctcgccgag gccggggaag
     1741 ttcgccggag ctgctgggcg tgccgctggc ccacaaggac atcttcgtca cgcgcgagct
     1801 gccgaccacc gccggctcga agatgctgga gggttaccga agcccgttcg acgcgaccgt
     1861 cgtttcacgc ctgaccggcg ccggcatggt gacgctgggc aagctgaact gcgacgaatt
     1921 cgcgatgggg tccggcaacg agaactcggc ctaccgtgcc gtcaggaacc cgtgggacct
     1981 gactcgcgtg ccgggcggtt cgtcgggggg ctccgccgcg gccgtggccg cgcgcctggt
     2041 cccggctgcg accggcaccg acaccggtgg gtcgatccgc cagccagcca gcttcagcgg
     2101 catcaccggc atcaagccga cctatggcgt gtgctcgcgc ttcggcatga tcgcgttcgc
     2161 ctccagcctg gatcaggccg gcccgatggc acgcagcgcc gaagactgcg cgctgctgct
     2221 cggcgcgatg agcggcttcg acgaacggga ttcgaccagc gccgaacgcc cgccccagga
     2281 ttacgcggct gccatgcgca aaccgcgagt gggcgccacg gcaacccagc cgctgacggg
     2341 cctgcgcgtg ggtctgccga aggaattctt cccggctgag ctgacttcgg gggtcgatgg
     2401 tgtggtgcgc gcggcactgg ccgaactgca gaggctcggc gccacgctgg tcgatgtcag
     2461 cctgccgcgc accgagctct cgattcccgt ctactacatc atcgcgcctg cagaggccag
     2521 ttcgaacctg agccgcttcg acggtgtgcg tttcgggcat cgcgcggcca agtacaccga
     2581 tctgctcgac atgtacaaga agacccgcgc cgaaggattc ggcgatgagg tcaagcgacg
     2641 catcatgatc ggcagctacg tgctgagcca tggctactac gacgcctact acctgcaggc
     2701 gcagaagctg cgcc
//
LOCUS       DAOORV010000313         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_46408_length_2667_cov_7.170368, whole genome shotgun sequence.
ACCESSION   DAOORV010000313 DAOORV010000000
VERSION     DAOORV010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46408_length_2667_cov_7.170368"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKV56_19695"
     CDS             complement(<1..316)
                     /locus_tag="PKV56_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX70069.1"
                     /translation="MKPTDLSLHVTNFLTRYLAGQRNLSPNTIKAYRDVFTLLLRFGR
                     DGRGIAVERLTLADIDVNFVEAFLDHLAQERHCSIRTLNQRLAVLHAFFRYVQSEVPE
                     RML"
     gene            complement(313..1308)
                     /locus_tag="PKV56_19700"
     CDS             complement(313..1308)
                     /locus_tag="PKV56_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX70070.1"
                     /translation="MAVPRKPATFTGNSPLAPLMAQYIQEKRACGYRYNDTALSLRRF
                     DAHLCRQGLQQVELPRSMTSSWLAKQPHENGKTQRDRINLVRHFALFMRRLDLPADVP
                     DHAVAAKESKSFLARVLTHEEVRRLFEAADRIEPKASTHMRHIVLPELLRMLYGCGLR
                     LEEALSLRMRDVDLAQGVLRINDTKFRKDRLVPPARPLVVRLQRYAAALGSRADDDYF
                     FPSPRGGRLDGGGVYRNYRELLHRSGIGHGGRGEGPRLHDLRHTFAVHVLLRWYREGA
                     DLQARMPVLATYLGHTSIEGTQDYLQMTAELYPEIVSRSDAAFSDVIPRRTGRPS"
     gene            complement(1292..2563)
                     /locus_tag="PKV56_19705"
     CDS             complement(1292..2563)
                     /locus_tag="PKV56_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOX70071.1"
                     /translation="MDSLNSDGSGALALEPLCQAAAQELLMRSYTVRTLNRYKRVWEQ
                     LADFARAKGQPAVYSRDLALRFEQAFGWRDGEVLVEGQRWRRHLVFGLKVLDDFARTG
                     TISRFVVETTGLRVPVGMHKALREYEQYARERRHLRPTSLAERMHSIAVFLDFLRTRG
                     LERLDTLQAEHLSAFVQTRTDWKPRTVKCVSSAVRQFLQYLFMRGMLPRDLGAAIPTV
                     RLASNAKVPSAWEPELVERLLAAVDRSSPKGRRDYAILLLASRLGLRLGDIKRLTLDH
                     LHWSTCTIEIVQNKTGEPLVLPLIEEVGAALIDYLRAGRPPVEHRHLFTNLTPPFDPI
                     CQNDRLYRVVAYWRELAGIKFKAPQRRGLHSLRHTLATRLLGAQTPFPVISAVLGHAS
                     PATTFIYAKADVETLRIAAIDPEEVCHGRAS"
BASE COUNT          465 a          854 c          895 g          453 t
ORIGIN      
        1 gcagcatccg ctccggcacc tccgactgca cgtagcggaa gaacgcgtgc agcaccgcca
       61 gacgctggtt cagggtgcgg atcgagcagt gccgctcctg cgccaggtga tcgaggaagg
      121 cctccacgaa gttcacgtcg atgtcggcca gagtcaggcg ctcgaccgcg ataccacgac
      181 cgtcacggcc gaagcgcagc agcagcgtga acacgtcgcg ataggccttg atcgtgttcg
      241 ggctcaggtt gcgctggcca gccaggtagc gcgtgaggaa gttcgtcacg tgcaacgaca
      301 ggtcggtcgg cttcatgacg gcctcccggt cctgcgtgga atgacatcgc tgaaggccgc
      361 atctgatcgc gagacgatct ccgggtacag ctcggcggtc atctgcagat agtcctgcgt
      421 gccctcgatc gaggtatggc cgagataggt ggccagcacc ggcatcctgg cctgcaggtc
      481 ggcgccctcc cgataccagc gcagcaggac gtgaacggcg aaggtgtgcc tcagatcgtg
      541 aaggcgaggc ccttctcctc ggccaccatg gccgatacca ctccggtgca gcaactcccg
      601 gtagttgcgg tacacgccgc caccgtccag acggcccccg cgcggcgacg ggaagaagta
      661 gtcatcgtcc gcacgtgacc ccagcgcggc cgcgtacctc tgcagacgca cgaccagtgg
      721 ccgggccggt ggcaccaggc ggtccttgcg gaacttggtg tcgttgatgc gcagcacgcc
      781 ctgggcgagg tccacgtcgc gcatgcgcag gctcagcgcc tcttcgagtc gcaggccgca
      841 tccgtacagc atccgcagca actctggcag gacgatgtgg cgcatgtggg tggacgcctt
      901 cggctcgatg cggtcagcgg cttcgaacag gcggcgcact tcctcatgcg tcaatacacg
      961 cgccaggaac gacttcgact ccttggcagc gactgcgtga tctggcacgt cggctggaag
     1021 gtccagcctg cgcatgaaca gggcgaagtg gcgaaccaga ttgatccgat cgcgttgtgt
     1081 cttgccgttc tcgtgcggct gcttggccag ccaggacgag gtcatggacc tgggcagttc
     1141 cacttgctgc aggccctgtc gacacaggtg agcgtcgaat cgacgcaggc tcaaggcggt
     1201 gtcgttgtag cgatagccac atgctcgctt ctcctggatg tactgcgcca tcagtggcgc
     1261 cagggggcta ttgccggtga atgttgcggg cttacgaggc acggccatgg cacacctcct
     1321 ccggatcgat ggcagcgatg cgcagcgtct ctacatcggc cttggcgtag atgaaggtgg
     1381 ttgccggcga ggcatgtccc agcacagccg agatgacggg gaacggggtc tgtgctccga
     1441 gcagccgcgt ggccagggtg tggcgcagcg agtgcaggcc ccgccgctgc ggtgccttga
     1501 acttgatccc ggccagctcc cgccagtacg ccacgacgcg gtagagccgg tcgttctggc
     1561 aaatcggatc gaacggcggc gtcaggttgg tgaagagatg gcggtgttct acgggtggtc
     1621 gccccgctcg caggtagtcg atgagtgcgg cgccgacttc ctcgatcaac ggcaggacca
     1681 gcggttcacc cgtcttgttc tgcacgatct cgatggtgca ggtggaccaa tgcagatggt
     1741 ccagcgtcag ccgcttgatg tcgcccaggc gcaggcccag gcgcgaggcg agcagcaaga
     1801 tggcgtagtc acgcctgccc ttcggtgagc tgcgatcgac cgcggcaagc agccgctcga
     1861 ccagttccgg ctcccacgcc gaaggcacct tggcattgct tgccagccgg accgtcggga
     1921 tggcggcacc gaggtcacgc ggcagcatgc cgcgcatgaa caggtactgc aggaactgcc
     1981 tcacggcaga cgagacgcac ttgacggtcc tcggcttcca gtccgtccgg gtctgaacga
     2041 aggcgctcag atgttcagcc tggagggtgt ccagcctctc caggccgcga gtgcgcagga
     2101 agtccaggaa gaccgcgatg ctgtgcatgc gctcggccag gctggtgggt cgaagatgcc
     2161 ggcgctccct ggcgtactgc tcgtactccc gcagggcctt gtgcatgccc accggcacac
     2221 gcagcccggt ggtctcaacc acgaaacggc tgatggtgcc ggtgcgcgcg aagtcgtcaa
     2281 gcaccttgag cccgaagacc aagtggcgcc gccaccgttg cccttccacc agaacttcgc
     2341 cgtcgcgcca accgaaagcc tgctcgaagc gaagcgcgag gtcgcgtgaa tagaccgccg
     2401 gctggccctt ggcacgggcg aagtcggcca gttgctccca cacccgcttg tagcggttca
     2461 gggttcggac ggtgtagctg cgcatcagca gttcctgcgc agcggcctgg cacagcggct
     2521 ccagcgccag cgcgccggac ccatcggagt tgagcgaatc catgattgtc tccacgttga
     2581 cggcggcccc agcggccacc ggggaggacg atcttggaag cgttatgcgc agtcttgtag
     2641 ccctacgtcg caggcggaac gcttggc
//
LOCUS       DAOORV010000314         2657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_46658_length_2657_cov_7.790161, whole genome shotgun sequence.
ACCESSION   DAOORV010000314 DAOORV010000000
VERSION     DAOORV010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46658_length_2657_cov_7.790161"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..873)
                     /locus_tag="PKV56_19710"
     CDS             complement(61..873)
                     /locus_tag="PKV56_19710"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOX70072.1"
                     /translation="MVDTGVGPHSALKVKRGLNTTAAESGRRFRDEDGHGSHVAGVIA
                     SAAAGWRRGEASAVELHAYRIFESGDPYASSFAIAAAIKDAANQGCDLVNLSIGDSMA
                     DDGIRDAVEFAWARGCVCIAATGNDGKGQVDYPARYTQAVAVSAIGLIGSWPAGTYLD
                     WTLSASRGKALGGQESFLASFSNRGAKVALTAPGVGVVSTIFSSRWGVMSGTSMATPI
                     ATGVLARRLAASPSVKDLPRDAARSAEIVKMALQAAEDIHLPAGMQGKGLAR"
     gene            complement(819..1625)
                     /locus_tag="PKV56_19715"
     CDS             complement(819..1625)
                     /locus_tag="PKV56_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70073.1"
                     /translation="MKLPQRPKRFIVIPRPSTPAVAPTSLQTMRLMHLGPRPPAKTAV
                     AKKAAPAAASRAARAATPTVSYGAPVKVVNRSPADGALLLEPQDHVTLDRLKAQAPQG
                     AKVLEEQWYSLERPARPWSTQSAALKKPRLRAGHTVTWSVTVVLDGGQRRRLPDALVT
                     VMTDEDKGIGVEGTTNRYGKASFVLSDKTKRMDAIYVDPLHSGWPMRLAEVLVVPGGV
                     EIAVVPIDLAVADGRGTVYGKPAAGSGRPFGSRWSTPGWGRTVRSRSNAD"
     gene            1813..2091
                     /locus_tag="PKV56_19720"
     CDS             1813..2091
                     /locus_tag="PKV56_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70074.1"
                     /translation="MIDSNKVKQIQDALKSFWNEQSMESVGEPTSIDDLVAAMDSMTA
                     VAALVDIEAIVGIELPCGEVIRAGGYDNERQFVEDITSRVCKYVQEHT"
     gene            2088..2450
                     /locus_tag="PKV56_19725"
     CDS             2088..2450
                     /locus_tag="PKV56_19725"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX70075.1"
                     /translation="MSTDDASTQRLEMAARLREAREYLGMSQEEVAVALELSRPAVTN
                     IESGSRKVEAVELERLARLYGRSIEYFLRGEQPKLDAKVAFAARTLHGLSSKDLDEVA
                     RFAEFLRSSGSKGSRSKK"
     gene            2447..>2657
                     /locus_tag="PKV56_19730"
     CDS             2447..>2657
                     /locus_tag="PKV56_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70076.1"
                     /translation="MNLRDQLLQASKRASAVLADVGAQQRVRDSGYSRIDPVAIAEGG
                     GVPVMVRPLDKLLGAFLNPEGSPGIL"
BASE COUNT          456 a          869 c          861 g          471 t
ORIGIN      
        1 aggaccttgg cgtcctgggc atcgctgttg aagaagacgt agcggctcat ggtgcccgga
       61 ttatcgggcc aagcccttgc cttgcatacc tgcgggcaaa tggatgtcct cggccgcctg
      121 cagggccatt ttcacgatct cggccgacct ggcggcgtcg cgcggcaagt ccttcacgga
      181 agggctcgcg gcgagccggc gcgccagcac gccggtggcg atcggcgtgg ccatggaggt
      241 gcccgacatc accccccagc ggctggagaa gatggtggaa accaccccca ctccaggcgc
      301 cgtcagcgcc accttggcgc cgcggttgga gaagctggcg aggaagctct cctgtccgcc
      361 cagcgccttg cctcgcgagg cgctcagcgt ccagtccagg taggtgccgg ccggccaact
      421 accgatgagg ccgatcgccg aaacggccac tgcctgcgtg tagcgcgccg ggtagtccac
      481 ctgccccttg ccgtcgttgc ctgtggccgc gatgcagacg cagccccggg cccaggcgaa
      541 ctcgaccgcg tcgcggatgc cgtcgtcggc catgctgtcg ccaatgctca ggttgacgag
      601 gtcgcagccc tggttggccg catccttgat cgccgcagcg atcgcgaagc tcgacgcgta
      661 ggggtcgcct gactcgaaga tccggtaggc atgcagctcg accgcgctgg cctcgccgcg
      721 gcgccagccc gccgctgccg aggcaatcac ccccgccacg tgcgacccgt gcccgtcctc
      781 gtcccggaac cgccgccccg actcggcggc ggtagtgttc agtccgcgtt tgaccttgag
      841 cgcactgtgc ggccccaccc cggtgtcgac caccgcgacc cgaacggtct gcctgaaccc
      901 gccgccggct tgccgtagac cgtgccgcgc ccgtcggcga ccgccaggtc gatgggtacc
      961 acggcgatct cgacgccgcc gggcacaaca agcacctcgg ccaggcgcat cggccaaccc
     1021 gaatgcagtg ggtcgacgta gatcgcatcc atgcgcttcg tcttgtcgct gagcacaaag
     1081 ctcgccttgc cgtaccgatt cgtcgtgcct tcgacgccaa tgcccttgtc ctcgtcggtc
     1141 atcacggtga ccagcgcatc cggcaggcgc cgccgctggc cgccgtcgag caccacggtc
     1201 accgaccagg tcacggtgtg accggcacgc agccgcggct tcttgagcgc cgcgctctgg
     1261 gtggaccacg gcctggccgg ccgctccagg ctgtaccact gctcctccag caccttcgca
     1321 ccctgcggtg cctgcgcctt caagcgatcc agcgtgacgt gatcctgagg ctccagcagc
     1381 aacgccccgt cggccggcga acggttgacc accttgaccg gcgcaccata agaaaccgtt
     1441 ggtgttgccg ctcgcgcagc acgcgaagct gcggccggag cggccttctt ggctactgca
     1501 gtcttggcag gcgggcgcgg gccaaggtgc atcaggcgca tcgtctgcag cgaggtcggc
     1561 gcgaccgctg gtgtcgaggg ccgcgggatg acgatgaatc gcttggggcg ttgcggcagt
     1621 ttcatgggca gtcctcctcc agtgttggcg ttgtgggtgg ccggtcaccg gcgcatgcca
     1681 ggaagtgtcc acgatgcgcg ggctacctcg gcgggcccca cgttgacaca gtccatgtgc
     1741 gttgacatca tcctacatgt acgatattct tacggctcag ggttgaaaat acatacacac
     1801 tcggggtaac agttgatcga ctcgaacaag gtcaagcaga tccaggacgc cctcaaaagc
     1861 ttctggaatg agcagtcgat ggagtccgta ggagagccaa cttctattga cgacttggtc
     1921 gctgctatgg actcgatgac cgccgtcgct gcgcttgtcg acattgaagc gattgtgggc
     1981 atcgaactgc cctgcggcga agtcattcgc gccggtggat acgacaacga gaggcagttc
     2041 gtcgaagaca taacttccag ggtgtgcaag tacgttcagg agcacacatg agcactgacg
     2101 acgcgtcgac ccagcgcctg gagatggccg caagactccg cgaggctcgc gagtacctcg
     2161 gaatgtcgca agaagaggtc gcagtggcac ttgagctgtc acgcccagcc gtgacgaata
     2221 tcgagtcggg cagcaggaaa gttgaggcag ttgagcttga acgactcgct cgcttgtacg
     2281 gtcgttcgat cgagtacttc cttcgcgggg agcagcccaa gctggatgca aaggtggctt
     2341 ttgcggcgcg tacgttgcat ggtctatcta gcaaggacct agacgaggtc gctcgcttcg
     2401 ccgagttcct ccggagttcg ggcagcaagg gatcgcggag caagaaatga acttgcgcga
     2461 ccaactcctc caagcttcaa agcgcgctag cgcggtgctg gctgacgttg gtgcgcagca
     2521 gcgggtgcga gattcgggct attcgcgcat cgatcccgtc gcgatagccg agggcggcgg
     2581 tgtgcctgtc atggtgcggc cgctagacaa gctgctcggg gccttcctga atcctgaagg
     2641 ttcaccggga attcttg
//
LOCUS       DAOORV010000315         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_47016_length_2642_cov_9.766911, whole genome shotgun sequence.
ACCESSION   DAOORV010000315 DAOORV010000000
VERSION     DAOORV010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47016_length_2642_cov_9.766911"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKV56_19735"
     CDS             complement(<1..884)
                     /locus_tag="PKV56_19735"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX70077.1"
                     /translation="MIIGAGMCGLYQLHKLLRMGADVTVLERGDDVGGTWYWNRYPGC
                     RFDSESYTYGYSFSKELLAEWDWTEHFSPQPENLRYVNHVVDKFGLRPHVQFGCNLTR
                     AVWDDEQQIWTLELADGRTLTCRFLLTAIGLLSAPTMPRLAGMDSFRGPSFHTYHWPK
                     EPVELAGKRVAVIGTGATGVQLISAIADKVDQLTVFQRRPNWCAPLHNAPISAAEMAS
                     IRADYAEIFKKCDNTPGGFIHGPDRRRVAEVPRAERLAFWERLYATPGFAIWLGNFRD
                     VLMDEAANAEFSAFVADK"
     gene            1121..1771
                     /gene="can"
                     /locus_tag="PKV56_19740"
     CDS             1121..1771
                     /gene="can"
                     /locus_tag="PKV56_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOX70078.1"
                     /translation="MCSIESLLKNNRAWSARCVAEDPGHFRRLAQQQAPRYLWIGCSD
                     SRVPANEILGLRPGEVFVHRNVGNLVVHSDLNCLTALQYAVEVLKVEHVLVVGHYGCG
                     GVQAVVEQRRTGLAENWLRHLEDVARKHADRLDDIGDRGNRASCLCELNVIEQVSNVC
                     RTTTVRRAWERGQRVEVHGLVYGLSDGLLRRVGESIFSEASCEPGYIEALAVLTVP"
     gene            1786..2046
                     /locus_tag="PKV56_19745"
     CDS             1786..2046
                     /locus_tag="PKV56_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70079.1"
                     /translation="MTHAEAEAAAPLGVVSEVVALLLFDQPCGFVALLGMIGLAGILA
                     QQVSDNFNDGLPALPAVVQAAVRRARPVVLTAIAAALACVPP"
     gene            complement(2434..>2642)
                     /locus_tag="PKV56_19750"
     CDS             complement(2434..>2642)
                     /locus_tag="PKV56_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX70080.1"
                     /translation="LSSLFSAAELTFESERALEVALQLFRKGSADFADCLHVALATQA
                     GEQPLWTFDKGAAKVSGAQLLAKA"
BASE COUNT          440 a          845 c          887 g          470 t
ORIGIN      
        1 atcttgtctg ccacgaaggc cgagaactcg gcgttggcgg cctcgtccat cagcacgtcg
       61 cggaagttgc ccagccagat ggcaaagccg ggtgtggcgt agagccgctc ccagaaggcc
      121 agccgttccg cgcgcggcac ttcggccacg cggcgccggt ccgggccgtg aatgaagccg
      181 cccggcgtgt tgtcgcactt cttgaagatc tcggcgtagt cggcccggat cgaggccatc
      241 tccgccgcag agatcggggc gttgtgcagc ggtgcgcacc agttgggccg gcgctggaag
      301 acggtgagtt ggtccacctt gtcggcgatc gcactgatca actgcacgcc ggtggccccg
      361 gtgccgatca cggccacccg cttgccggcc agttccaccg gctccttggg ccagtggtaa
      421 gtgtggaaag aagggccgcg gaagctgtcc atgcccgcca ggcgcggcat cgtcggcgcc
      481 gacagcaggc cgatggcggt gagcaggaag cggcaagtca gcgtacggcc gtcagccagt
      541 tcgagcgtcc agatttgctg ctcgtcgtcc cagacggcgc gcgtcaggtt gcagccgaac
      601 tggacgtgcg ggcgcagccc gaacttgtcg accacgtggt tcacatagcg caggttttcg
      661 ggctgcggcg agaagtgctc ggtccagtcc cactcggcca gcagttcctt cgagaacgag
      721 tagccgtagg tgtagctctc agaatcgaaa cggcagccgg ggtagcggtt ccagtaccac
      781 gtgccgccga catcgtcgcc gcgctccagc actgtgacgt cggcgcccat ccgcagcagc
      841 ttgtgcaact ggtaaaggcc gcacatgccg gcgccgatga tcacgaactg acgttcgggg
      901 gcttgcgtcc ggggcctcgc ggctggctct cttttgacca agactcatct ccgtattctt
      961 caggccgcgc cgggggaggg ccaccacaag cgctgcgacg gtgcttccag cagtctgcac
     1021 tggcgataca aaccaaacgt ttgttcgata tgatgccaga aggcccgtct ctacgggctc
     1081 gtcggtccat cccttccagc catcgatcga aggcctccac atgtgcagca tcgagtcctt
     1141 gctcaagaac aatcgggcct ggtcggcccg ctgcgtggcg gaggatccag gccattttcg
     1201 gcggcttgcg caacaacagg cgccgcgata cctgtggatt ggctgctccg acagccgcgt
     1261 tccggccaac gagatcctcg gcctgcgccc gggcgaggtc tttgtgcacc gcaatgtcgg
     1321 caacctggtg gtgcactccg acctcaattg tcttaccgcg ctgcagtacg ccgtcgaggt
     1381 cttgaaggtc gagcatgtgc tcgtggtcgg gcactacggc tgcggtgggg tacaggcggt
     1441 ggtcgagcag cgccgcacgg gcctggccga gaactggctg cggcacctgg aggacgtcgc
     1501 acgcaaacat gccgaccgcc ttgacgacat cggcgatcgc ggcaaccgcg cttcgtgcct
     1561 gtgcgagctc aacgtgatcg aacaggtcag caatgtgtgc cgcacaacga cggtgagacg
     1621 ggcctgggag cgcggccagc gcgtcgaggt gcatgggctc gtctacggcc tcagcgacgg
     1681 tctgttgcgt cgtgtcgggg agagcatctt cagcgaagcg agctgcgagc ctggttacat
     1741 tgaggcgttg gctgtgttga ccgtcccctg acaacttgtt gctggttgac tcacgctgaa
     1801 gctgaagccg cggcgcccct gggtgtcgtc agcgaggtgg tggcattgct gctgtttgac
     1861 cagccctgcg gcttcgtggc gctgctcggc atgatcggcc tggccggcat cctggcgcaa
     1921 caggtgtcgg acaacttcaa tgacggcttg ccggcgttgc cggccgttgt gcaggctgcc
     1981 gtgcgtcgcg cgcgtccggt ggtgctgacg gcaatcgccg cggcactggc ctgcgtgcca
     2041 ccttgacgat ctcgcccgga tggcgtactg cctccagcag atacaccgat gcgatcgcga
     2101 caagacccag cacgctgaac agcgccagtg ccgggctgag cacgaagggg agatgaagga
     2161 ccgtctccgc gcgctgccac gcgatcgtga gggcacaacc tacaagcgtg ttcggctgct
     2221 ggctggcccg agaacgcatt gccatcgtca tcgcgcccca gggcgatgcc gacgacagcg
     2281 agggctgaat tcttcgatgc gttcagttgc cggtgcgtgc tacagcgccc gacatcaagt
     2341 ccggcatcgc caagctcaag atcgatctac gcgagtacgg ccgattcatc tgcaccaccg
     2401 ccgacgacag tgtggctgcg aatccgtgcc gcctcatgcc ttggccagca gttgggcgcc
     2461 gctgaccttg gcggctccct tgtcgaaggt ccacagcggt tgctcgccgg cctgcgtggc
     2521 cagcgcgacg tgaaggcagt ccgcgaagtc cgccgagccc ttgcggaaca gctgcagcgc
     2581 gacctcgagc gcgcgctccg actcgaaggt cagctcggct gccgagaaga ggctggacag
     2641 gg
//
LOCUS       DAOORV010000316         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_48693_length_2576_cov_4.621579, whole genome shotgun sequence.
ACCESSION   DAOORV010000316 DAOORV010000000
VERSION     DAOORV010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48693_length_2576_cov_4.621579"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PKV56_19755"
     CDS             <1..1699
                     /locus_tag="PKV56_19755"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOX70081.1"
                     /translation="DAEVISQKLMLRAGFIKRLSAGIYTYMPIGLRVIRKVETIIREE
                     MSRAGAVELLMPLVQPAELWQETGRFDKMGAELLRVKDRHDRDFVIQPTSEEVITDIA
                     RQELRSYRALPKSFFHIQTKFRDERRPRFGVMRGREFTMKDAYSFDRDVESAAKSYDA
                     MYAAYVRIFERMGLTFRAVAADTGAIGGDRSHEFQVIADTGEDAIAYCATSSYAANVE
                     LAEAVLLNDVRPAATQALGKASTPQKSTCEDVAAFLGITLKSTVKSLVLATDDVLPAD
                     AGTRTTIWLLLIRGDHALNEVKVSKLAGLNAGHRFATAAEIGEHFGCKPGYLGPVGVS
                     GQVKVIADRTVAAMSDFVCGANEEGFHLTGVNWVRDLPEPHLVADIRNVVDGDPSPDG
                     QGELSLQRGIEVGHVFYLGTKYSAAMKATFLDENGKPKLMEMGCYGIGVTRILGAAVE
                     QLHDDRGMVWPAAIAPFAVVICPVGYDRFPNVKAAADDLYLSLREAGVDVVLDDRGER
                     PGAMFADWELIGVPHRVAIGERSLKENMVEYQGRQDLQSTPVPLSDIRGLLLGKLVP"
     gene            1696..2310
                     /locus_tag="PKV56_19760"
     CDS             1696..2310
                     /locus_tag="PKV56_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOX70082.1"
                     /translation="MTLRRRALLMTGLAAAVRCPQARAGGQAEEQLADAVRTALSAAV
                     GDIAPPRPSFDTMDQRLAYLRWLSAASDRLKRRKVDYEARVAFLETVWYESKRKGLDP
                     TLVLGLIQVESGFRKYAISGVGARGYMQVMPFWARLIGDGNVDSLFHMQTNLRFGCVI
                     LRHYLDIERGDQFLALGRYNGSRGRAEYPNLVFGARRQWDVMSA"
     gene            complement(2326..2576)
                     /locus_tag="PKV56_19765"
                     /pseudo
     CDS             complement(2326..2576)
                     /locus_tag="PKV56_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734507.1"
                     /note="catalyzes the formation of 2-octaprenylphenol from
                     3-octaprenyl-4-hydroxybenzoate; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
BASE COUNT          495 a          731 c          843 g          507 t
ORIGIN      
        1 tgacgccgaa gtgatcagcc agaaactgat gctgcgggcc gggttcatca agcgattgag
       61 cgccggcatc tacacctaca tgccgattgg tctcagagtg attcgcaagg tcgaaaccat
      121 cattcgggaa gaaatgtccc gcgcgggcgc cgtggagttg ctgatgccac tcgttcaacc
      181 tgctgagttg tggcaagaga cgggtcgttt tgacaagatg ggggcggaac tacttcgtgt
      241 caaagatcgg catgaccgcg atttcgtgat ccagcccacg agcgaggagg tcattaccga
      301 tatcgcccgg caggaactgc gcagctaccg cgcattgcca aagagctttt tccatattca
      361 gacaaagttc cgcgacgagc gccgtccgcg gttcggcgtg atgcgcgggc gcgaattcac
      421 gatgaaggat gcgtactcgt tcgatcgcga tgtcgaatcg gccgccaaga gttacgacgc
      481 gatgtacgct gcctatgtgc ggatcttcga acgcatgggc ctgacctttc gcgcagttgc
      541 tgcagacact ggtgcaatcg gcggggaccg ttctcacgag ttccaagtga ttgccgacac
      601 cggcgaggac gcgatcgcct attgcgccac gtcgagctat gcagccaatg tggaactggc
      661 tgaggcggtg ttgttgaatg acgttcgccc tgccgcgacg caggcgctgg gcaaggcatc
      721 gacaccccaa aaatcgacct gtgaagacgt agcagccttc ctgggaatca cgctcaaatc
      781 aaccgtcaag tcgctcgtgc tcgcgacgga cgatgtcttg cctgccgacg caggcacccg
      841 aacgaccatc tggctgctgt tgattcgcgg cgatcatgcg ctcaatgagg tcaaagtcag
      901 caaacttgca ggcctgaatg cggggcatcg ttttgccacg gctgcagaga tcggcgagca
      961 tttcggctgc aagcccggat acctgggccc ggtcggtgtg tcgggtcagg tgaaggtcat
     1021 cgctgaccgc acggtcgcag caatgagcga tttcgtctgc ggcgcgaacg aggaaggttt
     1081 tcacctgaca ggagtgaatt gggtgcgcga cctgccagag cctcatctcg tcgctgacat
     1141 ccgcaacgtg gtggatggcg atccgtctcc agacgggcag ggcgagttgt ccctccagcg
     1201 aggcattgaa gttggtcacg tcttctacct gggcaccaag tacagcgcgg caatgaaggc
     1261 gacgtttctc gacgagaacg gcaagccgaa gctgatggag atggggtgct acgggatcgg
     1321 cgtgactcgc atcctggggg ccgcagtcga gcagttgcat gacgatcgcg gaatggtctg
     1381 gcccgcggcc attgcaccgt ttgctgtcgt gatctgcccg gtcgggtacg accgatttcc
     1441 caacgtgaag gcggcggcgg acgacttgta tctttcgttg cgggaggccg gtgtcgacgt
     1501 ggttctcgat gaccgaggcg aacgtcctgg tgcgatgttt gccgattggg aactcatcgg
     1561 cgtgccgcat cgggtcgcga tcggcgagcg cagcctcaag gagaacatgg tcgagtacca
     1621 gggccgacag gacctccagt ccaccccggt gccgctttca gacatacgcg gcctgctgct
     1681 gggcaaactg gtgccatgac gttgcgacgg cgcgctctct tgatgaccgg tctcgctgct
     1741 gcggtccggt gcccgcaggc acgggcgggc ggtcaggccg aagagcagtt ggccgatgcg
     1801 gtgcgaacgg cgctttctgc ggcggttggc gatatcgccc ccccgagacc atcgttcgac
     1861 acgatggacc agcgtctggc ctacctgcgc tggctgagcg cggccagcga tcgcctgaag
     1921 aggcgaaagg ttgactatga agcccgggtc gcgtttctgg aaacggtctg gtacgaaagc
     1981 aagcgcaagg gattggaccc gaccctggtc ctggggttga tccaggtcga gagcgggttt
     2041 cgcaaatatg cgatcagcgg cgtcggagcc cgcggctaca tgcaggtcat gccgttctgg
     2101 gcccgtctga tcggggacgg caatgtggac agcctcttcc acatgcagac caacctgcgt
     2161 ttcggctgtg tgatcctgcg ccactacctc gacatcgagc gtggagatca attccttgcg
     2221 ttgggccgct acaacggcag ccgggggcgt gcggagtatc ccaatcttgt ctttggtgcc
     2281 cgccggcaat gggatgtgat gtctgcctga caccaggtca agggcctaga ggcctgacca
     2341 cgctttgggt gaggcggcct ggacgccgat caacgcaagt acccgctcca cggtttcatc
     2401 gaccatttcg tcgacgctga ctggcttcaa atagaaggca ggcaggggcg ggaaaatgat
     2461 gccgcccatc tcggtgacag cggtcatgtt gcgcagatgc gcgagattga gtggagtttc
     2521 tcatgccatc agaacgaggc gccgccgctc cttcagagtg acatcggccg cacggg
//
LOCUS       DAOORV010000317         2569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_48889_length_2569_cov_6.550119, whole genome shotgun sequence.
ACCESSION   DAOORV010000317 DAOORV010000000
VERSION     DAOORV010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48889_length_2569_cov_6.550119"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKV56_19770"
     CDS             complement(<1..578)
                     /locus_tag="PKV56_19770"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOX70083.1"
                     /translation="MNYLSTRGDRTPRGFSDILLEGLAPDGGLYLPESYPKIDDATLS
                     RWRGLAYADLAYEILSLYITDIPAGDLQALVRKTYTAETYGTPEITPLKPLDRAASTP
                     EGVDIYLQALSNGPTLAFKDMAMQLLGNLFEYELARRGERLNILGATSGDTGSAAEYA
                     MRGKKGVNVFMLSPNGRMSPFQQAQMFSLQDA"
     gene            complement(575..1903)
                     /locus_tag="PKV56_19775"
     CDS             complement(575..1903)
                     /locus_tag="PKV56_19775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOX70084.1"
                     /translation="MKPIRVGLLGIGTVGSGTFNVLRRNQEEIRRRAGRGIEITMVAD
                     LDTARAKAVVGESVTVVADAREVIADPEIDIVVELIGGYGIARTLVLEAIAAGKHVVT
                     ANKALLAVHGTEIFAAARGKGVVVAFEAAVAGGIPIIKALREGLTANRIQWIAGIING
                     TTNFILSEMRSKGLDFGVVLKQAQELGYAEADPTFDIEGVDAAHKVTLMSAIAFGIPV
                     QFDKAHVEGITQLQAADIGYAEHLGYRIKLLGITKRTNSGIELRVHPTLVPTKQLIAS
                     VEGAMNAVMVQGDAVGSTLYYGKGAGSEPTASAVIADLVDITRLHTADPHHRVPHLAF
                     QHDALVATPILPMDQTVTAYYLRLQVTDEAGVLARIAGILAEHAISIDALLQRPNDGS
                     TSAANAPAHTDVIILTHDTVEGNMNHAIAQMQSLPTVLAPIVRIRKEELS"
     gene            complement(1971..>2569)
                     /locus_tag="PKV56_19780"
     CDS             complement(1971..>2569)
                     /locus_tag="PKV56_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX70085.1"
                     /translation="DKTLYDGNVHTSIASLADDVLFVTYNGLSKNYRSCGYRAGWMVV
                     SGAKAHARDYIEGLNMLASMRLCSNTPGQLAIQTALGGYQSIKDLVAPGGRLCRQRDL
                     AYELLNQIPGVSVVKPKAALYMFPRLDPKMYPIEDDQQFAYDLLAAEKVLIVQGTGFN
                     WPDPDHFRLVFLPNCDDLTEAIGRIAHFLAQYRKRHAQ"
BASE COUNT          416 a          807 c          848 g          498 t
ORIGIN      
        1 ttggcgtcct gcaggctgaa catctgcgcc tgctggaagg ggctcatgcg gccgttcgga
       61 ctcagcatga atacattgac gcccttcttg ccgcgcatcg cgtactcggc cgcgctgccg
      121 gtgtctcccg aggtggctcc caggatattg agcctctcgc cgcggcgtgc cagttcgtat
      181 tcgaacaggt tgccgagcaa ctgcatcgcc atgtccttga aggcgagcgt cgggccgttg
      241 gacagcgcct gcagatagat gtcgaccccc tcgggcgtcg acgctgcccg gtcgagtggc
      301 ttcagcggcg tgatctctgg cgtgccgtat gtctctgctg tgtaggtctt gcgaaccagc
      361 gcctgcagat caccggccgg gatgtccgtg atgtacagcg acaggatctc gtacgcaagg
      421 tcggcatagg ccagcccccg ccaacgggac agcgtcgcgt cgtcgatctt cggataagac
      481 tcggggaggt agaggccgcc gtcaggcgcc aggccttcaa gcaggatgtc ggaaaagccg
      541 cgcggggtgc ggtcaccccg ggtactgagg taattcaact gagctcttcc ttgcggatgc
      601 gcacgatcgg ggcgagcacc gtcggcagcg actgcatctg ggcgatcgcg tggttcatgt
      661 tgccctcgac cgtgtcgtgc gtgaggatga tcacatcggt gtgtgcaggg gcgttcgcgg
      721 ccgaggtgct gccatcgttc ggacgctgca gcagtgcatc gatcgagatg gcgtgctcgg
      781 ccaggatccc ggcgatgcgg gcgagcacgc cagcttcgtc ggtgacctgc agccgcaggt
      841 agtacgccgt gacggtctga tccatcggca ggatcggtgt cgcgaccaga gcgtcgtgct
      901 ggaacgcgag gtgcggcacg cggtggtgcg ggtcggcggt gtgcagacgg gtgatgtcga
      961 ccagatcggc gatcaccgcc gaagccgtgg gctcggatcc cgcgcccttg ccgtagtaca
     1021 gggtgctgcc caccgcgtcg ccctgcacca tcacggcatt catcgcgccc tcgacactgg
     1081 cgatgagttg cttcgtcggg accagcgtcg ggtgcacgcg cagttcgatg ccgctgttcg
     1141 tccgcttggt gatgccgagc agcttgatgc ggtagcccag atgctcggca tagccgatgt
     1201 cggcggcctg cagttgagtg atgccttcga cgtgcgcctt gtcgaactga accgggatgc
     1261 cgaacgcgat cgcgctcatc agcgtcacct tgtgcgccgc atcgacaccc tcgatgtcga
     1321 acgtcggatc agcttccgcg tagccgagtt cctgcgcctg cttcagcacc acgccgaaat
     1381 cgagcccctt ggagcgcatc tccgacagga tgaagttggt ggtgccgttg atgatcccgg
     1441 cgatccactg gatgcggttg gccgtcagcc cctcgcgcag cgccttgatg atcgggatgc
     1501 ccccagccac cgcagcctcg aatgcgacga cgacgccctt gccacgggcg gcagcgaata
     1561 tctcggtgcc atgcaccgcc agcaatgcct tgttggccgt gaccacatgc ttgcccgcag
     1621 caatggcttc gagcaccagc gtgcgagcga tgccatagcc cccgatcagc tcgacgacga
     1681 tgtcgatctc gggatcggcg atcacttcgc gcgcgtcggc caccaccgta accgactctc
     1741 cgacgaccgc cttcgcccgc gccgtgtcga ggtctgcgac catcgtgatc tcgatgccgc
     1801 gaccagcgcg gcgcctgatt tcctcctggt tgcgacgcag cacgttgaac gtgccgctgc
     1861 caacggtgcc gatgcccagc aggccaactc gtatcggttt catcttgtgt gccccagcgg
     1921 gcgtatggat tgcaaaaagg gattcggttc gagccccgtt cgatgtcgac tcactgcgcg
     1981 tgccgcttgc ggtattgcgc aagaaagtgg gcgatgcgcc cgatcgcttc agtcaggtca
     2041 tcgcagttcg gcaggaagac cagccggaag tgatcggggt cgggccagtt gaagccggtc
     2101 ccctggacga tcaggacttt ctctgcggcg agcaggtcgt aggcgaactg ctggtcgtcc
     2161 tcgatcgggt acatcttggg gtcgagcctg gggaacatgt acagcgcagc cttcggcttc
     2221 acgacgctga cacccgggat ctggttcagc agttcgtagg ccagatctcg ctggcgacac
     2281 aggcgtccgc cgggagcgac gagatccttg atgctctggt agccgccgag cgcggtctgg
     2341 atcgcaagct ggccgggcgt gttcgagcac agccgcatcg acgccagcat gttcaggcct
     2401 tcgatgtagt cgcgtgcatg cgccttggcg ccggacacca ccatccaccc ggcgcggtag
     2461 ccacatgagc ggtagttctt gctcaggccg ttgtaggtca cgaacaggac gtcatcggcc
     2521 agcgaggcga tgctggtgtg cacgttgccg tcgtacagcg tcttgtcgt
//
LOCUS       DAOORV010000318         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_49757_length_2537_cov_8.962127, whole genome shotgun sequence.
ACCESSION   DAOORV010000318 DAOORV010000000
VERSION     DAOORV010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49757_length_2537_cov_8.962127"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1546
                     /gene="istA"
                     /locus_tag="PKV56_19785"
     CDS             23..1546
                     /gene="istA"
                     /locus_tag="PKV56_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057390712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOX70086.1"
                     /translation="MDMIGRIRRLHARKNKSEREISRMTGLSRNTVAKWLHGEVDGPP
                     KYRRGEQPNKLTAFHEALRQALKADARRPKRERRTAKALYAEIRGAGYDGGYTRVTDF
                     IRAWRQGEGQSASANAFVPLAFELGEAFQFDWSEEGLVVGGIYYRMQVSHMKLCASRA
                     FWLVAYPSQGHEMLFDAHTRSFAALGGVARRGIYDNMKTAVDKVKKGKGRIVNERFAT
                     MCAHYLYDPDFCNVASGWEKGVVEKNVQDSRRRIWIDAAKQRFGSFSELNAWLGQRCR
                     ALWDEVRHPEHEQFSVAEMLEHERAHLMPMPAPFDGYVEKPARVSSTCLVSVARNRYS
                     VPCELFGQMVSTRLYPGSVVVVANDHIVARHDRLSNAGETRYDWQHYIPLLQRKPGAL
                     RNGAPFADMPEPLQRLRRGLLRNPGGDRVMAQVLAIVLSAGLDAVLVAVELALETGPP
                     GKVSVEHVVNVLGRLNAVPMPQTAATQLKVSTPPLANTARYDSLRADNATEEAGHDA"
     gene            1536..2345
                     /gene="istB"
                     /locus_tag="PKV56_19790"
     CDS             1536..2345
                     /gene="istB"
                     /locus_tag="PKV56_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOX70087.1"
                     /translation="MTRDVTVELKALRLHGMAGAWADLVEQGTNAGVDSSRWLIEHLL
                     QAEGTDRAMRSVSHQMHAAKFPVHRDIAGFDFEVSPVDRKLVSTLAATNFTDDAHNVV
                     LVGGPGTGKTHLATAIGVSGITRHGKRVRFFSTVDLVNALEQEKAQGKAGRIALSLLR
                     MDLVILDELGYLPFSQAGGALLFHLLSRLYEHTSVMITTNLDFAEWSSVFGDAKMTTA
                     LLDRLTHHCHIVETGNESIRFSRSTAEAKKRIKAREQARKGSKSELPQDDF"
BASE COUNT          464 a          801 c          892 g          380 t
ORIGIN      
        1 cagaggacga ggagtgatca ccatggacat gattggcagg atccggcgtt tgcatgcccg
       61 gaagaacaaa tcggagcgcg agatctcgcg catgacgggg ctgtcgcgca acacggttgc
      121 gaagtggctg cacggcgagg tggatggccc gccgaagtac cggcgcgggg agcagccgaa
      181 caagctcact gcgttccacg aggcgctcag gcaggcgttg aaggccgacg cccggcggcc
      241 caagcgcgag cgtcgcacgg ccaaggcgct gtacgccgag atcaggggcg ctggctacga
      301 cggtggctac acgcgggtga ccgacttcat ccgggcgtgg cgccagggcg aaggtcaatc
      361 tgcgagcgcc aacgccttcg tgccgctggc cttcgagctc ggcgaagcct tccagttcga
      421 ctggagcgag gaagggctgg tggtgggcgg gatctactac cgcatgcagg tctcgcacat
      481 gaagctatgc gcttcgcggg ccttctggct ggtggcctat ccgagccaag gccatgagat
      541 gttgttcgat gcccataccc gcagctttgc agccctgggc ggcgtggccc ggcgcggcat
      601 ctacgacaac atgaagaccg cggtggacaa ggtcaagaag ggtaagggcc ggatcgtcaa
      661 tgagcgcttc gcgaccatgt gtgcgcacta cctgtacgac ccggacttct gcaacgtcgc
      721 cagcggctgg gagaagggcg tcgtcgagaa gaacgtgcag gacagccgcc ggcgcatctg
      781 gatcgacgcg gccaagcagc gcttcggcag cttcagcgaa ctcaacgcct ggctgggcca
      841 gcggtgccgg gcgttgtggg acgaggtgcg ccaccccgag cacgagcagt tcagcgtggc
      901 cgagatgctc gaacacgagc gggcgcatct gatgcccatg ccggcgccgt tcgatggcta
      961 cgtcgagaag cccgcgcgcg tgtccagcac ctgcctggta tcggtggcgc gcaaccgcta
     1021 ctcggtgccg tgcgagctgt tcgggcaaat ggtcagcacc aggctgtacc cgggcagcgt
     1081 cgtcgtggtg gccaacgacc acatcgtcgc gcgccacgac cgcctgagca acgccggcga
     1141 gacgcggtat gactggcagc actacatccc gctgctgcag aggaagcccg gcgcgctgag
     1201 gaatggcgcg ccgttcgccg acatgcccga gccgctgcag aggctgcgcc gtgggctgct
     1261 gcgcaacccg ggcggcgacc gcgtgatggc gcaggtgctg gccatcgtgc tgagcgccgg
     1321 gctggacgcg gtgctggtgg cggtggaact ggcgctggag accgggccgc ccggcaaggt
     1381 gagcgtggag cacgtggtca acgtgctggg gcgcctgaat gcggtgccga tgccgcagac
     1441 ggctgcgacg cagctgaagg tgagcacacc gccgctggcc aacacggcgc gctacgacag
     1501 cctgcgtgcc gacaacgcca ccgaggaggc cggccatgac gcgtgacgtg acggttgaac
     1561 tcaaggcgtt gcgtctgcat ggcatggccg gcgcctgggc cgacctggtg gagcaaggaa
     1621 cgaacgcagg ggtggactcc tcgcgctggt tgatcgagca cttgctgcag gccgagggca
     1681 cggatcgcgc catgcgctcg gtcagccacc agatgcacgc cgcgaagttc ccggtgcatc
     1741 gcgacatcgc cggcttcgac ttcgaggtct cgccggtaga tcgcaagctt gtgagcacac
     1801 tggccgcaac caacttcaca gacgacgcac acaacgtcgt gttggtgggt gggccgggca
     1861 cgggcaagac gcatctggcc acggccatcg gcgtatcggg catcacccgg catggcaagc
     1921 gcgtgcggtt cttctcgacg gtcgacctgg tcaatgcact ggagcaggag aaggcgcaag
     1981 gcaaggccgg gcgcattgca ttgagcttgc tgcgcatgga cctcgtcatc ctggacgagt
     2041 tgggctacct gccgttcagc caggccggcg gggcgttgct gttccacctg ctcagtcgcc
     2101 tgtatgaaca caccagcgtg atgatcacga ccaacctgga cttcgcggag tggtccagcg
     2161 tgttcggcga cgcgaagatg accaccgcgt tgctggaccg gctcacgcac cactgccaca
     2221 tcgtggagac gggcaacgag tcgatccgct tcagccgcag tacggcagag gccaagaagc
     2281 gcatcaaggc gcgcgagcag gcccgcaagg gcagcaagtc cgagctgccg caggacgact
     2341 tctgaagcgg cgccgcgcgg ggaaatccgc tacgggctac gccctgcgcg gcttcccccg
     2401 cgcgctcaac tcaacatcga ggaggtcaaa gagccaggac agacctacac ttatccaccg
     2461 ccggccctct gaaggccggc ccccacccct ggctcaatat tcggtcggca cggtggctca
     2521 gatttggatc ggcgccg
//
LOCUS       DAOORV010000319         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_51234_length_2486_cov_5.121349, whole genome shotgun sequence.
ACCESSION   DAOORV010000319 DAOORV010000000
VERSION     DAOORV010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51234_length_2486_cov_5.121349"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKV56_19795"
     CDS             complement(<1..535)
                     /locus_tag="PKV56_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOX70088.1"
                     /translation="MAAKDVIFGGEARARMVEGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDIAGDGTTTATVLAQAIVRE
                     GMKYVAAGMNPMDLKRGIDKAVTALVEQLKKQSKPTTTSKEIAQVGTISANSDHEVGR
                     IIAEAMDKVGKEGVITVE"
     gene            complement(584..874)
                     /locus_tag="PKV56_19800"
     CDS             complement(584..874)
                     /locus_tag="PKV56_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOX70089.1"
                     /translation="MKLRPLHDRVIVKRLEQETKTASGIVIPDNAAEKPDQGEVLAVG
                     PGKRNDKGDFIALNCKVGDRVLFGKYSGQTVKVDGDELLVMREEDLFAVVEK"
     gene            complement(1064..1273)
                     /locus_tag="PKV56_19805"
     CDS             complement(1064..1273)
                     /locus_tag="PKV56_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOX70090.1"
                     /translation="MTTSPVPERIVRCPGCGGDSAYSSTNPYRPFCSQRCKSTDFGAW
                     ASEGYRVGASASPEEPDDLAPPPEH"
     gene            complement(1270..2025)
                     /gene="zapD"
                     /locus_tag="PKV56_19810"
     CDS             complement(1270..2025)
                     /gene="zapD"
                     /locus_tag="PKV56_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOX70091.1"
                     /translation="MVLYEYPFNESIRTMLRLEHLFDRLGQLAPRETAVDHHYAIATI
                     FEIMEVASRADLKSDLLKDLERQKAQLNSYRGNPSISEKALDEVVDKIDHAFAGLNQL
                     PGKAGHALTANEWLMSIRSRISIPGGTCEFDLPGYYAWQQHDPSRRRADLARWIALLL
                     PLAEALTVLLGLLRDSGVAQRVNAPAGQFQQSLPAGRTYHLMRVRLDPQGGLVPEITG
                     HRLMVSVRLMRQDSEGRLRPDTADGSFELTLCS"
     gene            complement(2093..>2486)
                     /gene="coaE"
                     /locus_tag="PKV56_19815"
     CDS             complement(2093..>2486)
                     /gene="coaE"
                     /locus_tag="PKV56_19815"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430449.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOX70092.1"
                     /translation="FDDPSARRRLEAILHPMISSEAEREAASHTAARVIVFDVPLLVE
                     SGRWIDRVDRVLVVDCCEQTQIERVVRRSGWSEDTVRSVIAAQATREQRRSVADDLIV
                     NDAISPTQLAERVASLWRAWCTPQTGPL"
BASE COUNT          429 a          749 c          781 g          527 t
ORIGIN      
        1 cttcaacggt gatgacgcct tccttgccga ccttgtccat cgcctcggcg atgatgcggc
       61 cgacttcgtg gtcgctgttc gccgagatcg tgccgacctg agcgatttcc ttggacgtcg
      121 tggtgggctt cgactgcttc ttgagctgtt ccaccaaggc ggttacggcc ttgtcgatgc
      181 cgcgcttcag gtccatcggg ttcatgcccg ccgccacgta cttcatgcct tcgcgcacga
      241 tggcctgggc cagcacggtg gcggtggtgg tgccgtcacc ggcgatgtcg ctggtcttgg
      301 aagcgacttc cttgaccatc tgagcgccca tgttctgcag cttgtccttg agctcgattt
      361 ccttggcgac cgaaacgccg tccttggtca cggtcggggc gccgaacgaa cgctcgagca
      421 caacattgcg acccttgggg cccagcgtga ccttgaccgc attggccagg atgttcacgc
      481 cttcgaccat gcgggcacgg gcttcaccgc cgaaaattac gtcttttgct gccatgtgtg
      541 tatctcctga atattgagtg gatgcgtcgc gcgggagaag tgattacttc tcgaccacgg
      601 cgaacaggtc ttcttcgcgc atcacgagca gctcatcgcc atcgaccttg acggtctgac
      661 cgctgtactt gccgaacagg acgcggtctc caaccttgca gttcagcgcg atgaagtcac
      721 ccttgtcgtt gcgcttgccc gggccgactg ccagaacttc gccctggtcg ggcttctcag
      781 cagcgttgtc ggggatgacg atcccggagg cggtcttggt ttcttgttcc aggcgcttga
      841 cgatcacgcg atcgtgcaga ggacgaagtt tcatagcatc tccttgaggt gtaggaacgg
      901 tgttcattcg ttttacgggc gccgacgtcg tcatgaccaa tcgggctgcg ttgattcttc
      961 ggcaggagcc ggaatccgtt agcactcagt tagatcgagt gctagcagat caattatagg
     1021 gacgtgagac gacaattcaa gaggccgcgc gggaccggtg cggtcaatgt tccggcggcg
     1081 gggccaagtc gtcgggctct tccggtgatg cggatgcgcc tacccggtag ccttcgctgg
     1141 cccaggctcc aaagtcggtg ctcttgcagc gctggctgca aaaaggccgg taggggttgg
     1201 tgctcgagta ggcgctgtca cccccacaac cggggcagcg gacgatgcgc tcgggaaccg
     1261 ggcttgtcgt caagagcaaa gggtcagctc gaagctgccg tcagcggtgt cggggcgcag
     1321 gcgaccttcc gagtcctgac gcatcagccg aaccgagacc atcagtctgt gtccggtgat
     1381 ttccgggacc agcccgcctt gcgggtcgag ccgcacgcgc atcaggtgat aggtccggcc
     1441 ggccggcaga ctttgctgga actgccccgc cggcgcattg acgcgttgcg ccacgcctga
     1501 atcccgcagc agccccagca ggacagtgag cgcttctgcc aatggcagaa gcagcgcgat
     1561 ccagcgtgcc aggtcggcgc ggcgccgtga gggatcgtgc tgctgccatg cgtaatagcc
     1621 cggcagatcg aactcacagg taccaccagg gatgctgatt cgactgcgga tgctcatcag
     1681 ccattcgttg gcagtgagcg cgtgcccggc ctttcccggc agttggttca gccccgcgaa
     1741 ggcatggtcg atcttgtcga cgacctcgtc cagggctttc tcggagatcg aaggattgcc
     1801 gcgatagctg ttgagctgcg ctttctgtcg ctcaagatcc ttcagcagat ccgacttgag
     1861 atctgcacgc gacgccactt ccatgatctc gaagatcgtt gcgatcgcgt aatggtgatc
     1921 gacggcggtt tcacgcggcg ccagttgacc aagtcggtcg aacaggtgtt cgagccgcag
     1981 catggtgcgt atgctctcgt tgaacgggta ctcgtaaagg accaaagggc ctgcctttaa
     2041 cgtctgtgga aactgcggtg gtcggactgg atttccgatc cggtcgattg tttcacagag
     2101 gtccggtctg gggtgtgcac catgcacgcc acaacgaggc cacgcgctcg gcgagttgag
     2161 tgggcgaaat ggcgtcgttg acgatcagat cgtctgcaac ggaccggcgc tgttcgcgag
     2221 tggcttgtgc cgcgatgacg gatcgaaccg tatcctccga ccaccccgag cgtctgacca
     2281 cgcgctcgat ctgcgtttgc tcgcagcaat cgaccaccag aacgcggtcc acccgatcga
     2341 tccaccggcc tgactcaacc agcaaaggga catcgaacac gatcacccgg gccgctgtgt
     2401 gtgatgcggc ttcacgctcg gcttcgctcg agatcatcgg gtggaggatc gcttcgagtc
     2461 gccgccgggc actcgggtcg tcgaag
//
LOCUS       DAOORV010000320         2408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_53618_length_2408_cov_9.235869, whole genome shotgun sequence.
ACCESSION   DAOORV010000320 DAOORV010000000
VERSION     DAOORV010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53618_length_2408_cov_9.235869"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..799)
                     /locus_tag="PKV56_19820"
     CDS             complement(224..799)
                     /locus_tag="PKV56_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX70093.1"
                     /translation="MAGSSQYTAILDANTLYPAPLRDLLLSLAIEGLYHARWTARIHE
                     EWVRNLIGNRPELKPRLGALVELMNRSVPDCLVENYEGLIAGLVLPDPDDRHVLAAAI
                     AGHADAIVTFNLKDFPADALDSHQIEAIHPDDFVLNQLELRPFEALAAVKKMRARLNR
                     PPQTAAELIATLERSGLPASAAHLRQAQALI"
     gene            complement(799..1266)
                     /locus_tag="PKV56_19825"
     CDS             complement(799..1266)
                     /locus_tag="PKV56_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX70094.1"
                     /translation="MSTCDLLEPPASEREAEMAKVAQRCLMAALDHSRAQRIALVDEK
                     GEIADAPVLELPPQALRFFAEMLGMMAQRRPVMLVPQNHELTTQDAAALLNVSRPFVI
                     KEIEAGRLKCRKVGRHRRIEFEELRRYQAAQRAQSEEALQKLADLSQDLSLGY"
     gene            1738..>2408
                     /locus_tag="PKV56_19830"
     CDS             1738..>2408
                     /locus_tag="PKV56_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HOX70095.1"
                     /translation="MSESGSEVDGACPDRCLRVEATLIAKLNLADFQNAVPMLRELRI
                     VNDTELAVQDLELRITSEPAFLKPKIWRVDAVAAGESRRIPDLDVQLDGALLTRLTEA
                     EQASVTLTLHHRGRDEPDLARFEKTVELLPRNQWGGLSHLPDLVAAFVQPNEPAVERL
                     LKQAAEVLRKNGKSAALDGYKGGSKRAWELSSAIWSAVAAMGLDYALPPASFEHTGQK
                     VRGPG"
BASE COUNT          411 a          750 c          811 g          436 t
ORIGIN      
        1 ggtcgcttcg ctcttgcgcc agcgcatgcc ttccagttcg ccaccgtcgt ccgggatcag
       61 gctcatcaaa aacccccata tgaatcagcc acttggccaa ttgtgccttt tgcggatcgg
      121 tttggaaggc caaaatcatc taccttgata ataaggatta tcaaggtatt gggccgtctg
      181 aaagggcagg gagggcgccc ggagcagggc gccggcagca gcttcagatc agggcctgcg
      241 cctggcgcag gtgcgccgcg ctcgccggaa gacccgagcg ctccagcgtt gcgatgagct
      301 cggccgccgt ctgcggcggg cggttcaggc gtgctcgcat cttcttgaca gccgccagcg
      361 cctcgaaggg gcggagctcg agttggttca gcacgaaatc gtccgggtgg atggcttcga
      421 tctggtgcga atcgagggca tccgccggga agtccttcag gttgaaggtg acgatcgcgt
      481 cagcgtgacc ggcgatggcg gcagccagca cgtggcggtc atcggggtcc ggcaggacca
      541 ggccagcgat cagtccttca tagttctcaa cgaggcagtc cggcaccgaa cggttcatca
      601 gctcgaccag cgcgccgagc cgcggcttca gctccggcct gttgccgatc aggttccgaa
      661 cccactcctc gtgaatccgg gccgtccatc gggcgtggta caggccctcg atcgccaggc
      721 tcagcagcag gtcgcgcagc ggcgccgggt agagcgtgtt cgcgtcaagg attgcggtgt
      781 actgcgacga gccggccatc agtagcctag gctcagatcc tgggacaggt cagcgagctt
      841 ctgcagcgcc tcttcggact gcgcgcgctg cgccgcctgg tagcgccgca gttcttcgaa
      901 ctcgatccgc cggtgccggc ccaccttgcg gcacttcaac cgcccggcct cgatctcctt
      961 gatcacgaag ggccgcgaca cattaagcag cgctgcagcg tcctgagtgg tcagctcatg
     1021 gttctgcggc accagcatca ccggacggcg ttgcgccatc atgcccagca tctcggcgaa
     1081 aaagcgcagc gcctgtggcg gcagttccag gaccggcgcg tcggcgatct cgcccttctc
     1141 gtcgacgagg gcgatgcgct gcgcgcgcga gtggtccagc gcggccatca ggcagcgctg
     1201 ggcaaccttg gccatctcgg cctcccgttc agaggccggg ggttcgagca ggtcacacgt
     1261 ggacatcgtg tctccaaagc gatgggtcgg accagtgccg acaggcctct actctacacc
     1321 ctattcgaat tattcgcaac attcgaagcc atcgaatgcc gggggtgcct ggccggcgtg
     1381 tgaaccggtg ttcaggcggt gattcggcat tggcagtccc ttgtctcagc gaggaagcac
     1441 gcagcgcggc agcgggcttc cggcaccgct gaggtgcagc cctatgcacg tcaaacggcg
     1501 ctgctcgacg cgtgcgtact gtgtccgtgg gccgtggcag atgttctgat gccagctgcc
     1561 gatggccaaa tcgacggtat ctgtgctggc tggcgcgggc gatcggaccg gtcgagctct
     1621 gtgacaagat gcgttgcatg agtggtctgg agtcgctgac atggcgcatc agcagatcgc
     1681 gaaggggagg gccctcgacg gcccacggta ctagcttggt ctgcagggtg gcccgtcatg
     1741 tcggaatccg gttctgaagt tgatggggca tgccccgaca ggtgtctgcg tgttgaagcc
     1801 acgctgatcg ctaagctcaa ccttgcggat ttccagaacg cggtgccgat gctcagggaa
     1861 ctgcgcatcg tcaacgacac cgagttggcg gtccaagatc tggagttgcg catcacgtcg
     1921 gagccggcct tcctgaagcc gaagatctgg cgggttgatg cagtggcggc aggcgaaagc
     1981 cgccgaatcc ccgatctgga tgtccaactg gacggcgcgc tgctgacccg gctgaccgag
     2041 gccgagcagg cctcggtcac gctgacattg catcatcgtg gtcgtgatga gccggacctg
     2101 gctcgtttcg agaaaacggt cgagttgctg ccccgcaacc agtggggcgg cctgtcccat
     2161 ctgcccgacc tggtggcggc ctttgttcaa cccaacgagc ctgcggttga gcgcctgctc
     2221 aagcaggctg ccgaggtgct ccgcaagaac ggcaagagcg cggcgctgga cggctacaag
     2281 ggcggcagca agcgtgcctg ggagctgagc tcggcgatct ggagcgctgt ggccgccatg
     2341 ggcctggact acgccctgcc gccggccagc ttcgagcaca ccgggcagaa ggtgcgcggg
     2401 ccgggcca
//
LOCUS       DAOORV010000321         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_59966_length_2232_cov_7.903996, whole genome shotgun sequence.
ACCESSION   DAOORV010000321 DAOORV010000000
VERSION     DAOORV010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59966_length_2232_cov_7.903996"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2206)
                     /locus_tag="PKV56_19835"
     CDS             complement(<1..2206)
                     /locus_tag="PKV56_19835"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX70096.1"
                     /translation="MKDPVCGMTVTEQSPHRAEHEGRPYYFCSAKCLTKFTAEPARYA
                     NPAPPVPSEPPAVEAAAGTIYTCPMHPEIRQDHPGNCPKCGMTLEPVLPALDEGENPE
                     LADFKRRFWWTLPLTVVVTVLAMFGHRLGWFDMARQSWIELVLTLPIVLWAGAPFFVR
                     GAQSVVNRSPNMWTLISLGTGSAFVYSIVATIAPQVFPDSFVSMGRVAVYFEAAAVII
                     SLTLLGQLLELKARSQTSAAIKSLLGLSPKTARRIAADGSEEDIPLANVHVGDKLRVR
                     PGEKVPVDGVVVEGSSALDESMLTGEPLPVTKRVGDKLIGATLNTSGALVMQSEHVGS
                     ATVLSQIVQMVALAQRSRAPMQRMADSVAGYFVYGVVAAALLTFFAWGLFGPQPSWVF
                     GLINAVSVLIIACPCALGLATPMSIMVATGRGATHGVLFRDAAAIENLRKVDTLIVDK
                     TGTLTEGRPTFEKAVGVSGFEPNEVLRVAASLDQGSEHPLAAAIVAAAREKKLALDKP
                     EQFDSASGIGVRGSVAGKAVALGNTAWMAQLGVDVASLAAQAEALRAEGASVMHLAVD
                     AKLAGILAVSDPVKTTTAYALASLRRANIRVVMATGDGLTTAQAVGKRLGIEEVHGEV
                     KPADKLKLVEKLQSEGRIVAMAGDGINDAPALARADVGIAMGTGTDVAMNSAQLTLVK
                     GDLRGIATARALSVATVANMKQNLAFAFVYNALGVPLAAGVLFPFTGWLLSPM"
BASE COUNT          367 a          772 c          733 g          360 t
ORIGIN      
        1 tcatcggcga cagcagccag ccggtgaacg gaaacagcac gcctgcggcc agcggcacgc
       61 ccagcgcgtt gtagacgaag gcgaaggcca ggttctgctt catgttcgcg acggtggcga
      121 ccgacagggc gcgtgccgtg gcgatgccac gaaggtcgcc cttgacgagg gtgagctgcg
      181 cgctgttcat cgccacgtcg gtgcccgtgc ccatggcgat gccaacatcg gcgcgggcca
      241 aggccggtgc atcgttgatg ccatcgcccg ccatcgcgac gatccgacct tcggattgca
      301 gcttctcgac cagcttgagc ttgtctgccg gcttcacctc gccgtgcact tcctcgatgc
      361 ccagtcgctt gcccacggct tgtgccgtgg tcaggccatc tccagtggcc atcacgacgc
      421 ggatgttcgc cctgcgcagg gacgccaatg cgtaggcggt ggtcgtcttg acaggatccg
      481 atacggcaag gatgccggcc agtttggcgt ccacagccag atgcatcacg ctcgcccctt
      541 cggcgcgcag cgcttcggcc tgtgcagcca aagaggccac gtcgacgccg agttgcgcca
      601 tccacgcggt gttgcccagg gccactgcct tgcctgccac gctgccgcgc actccgatcc
      661 ctgaggcgga gtcgaattgc tcgggcttgt ccagtgcgag cttcttctcg cgcgccgcgg
      721 ccacgatcgc cgcggccagc gggtgctcgc tgccctggtc gaggctggcc gccacgcgca
      781 acacctcatt cggctcgaag ccgctcacgc cgactgcctt ctcgaatgtc ggcctgccct
      841 cggtcaatgt cccggtcttg tcgacgatca gcgtgtcgac cttgcgcagg ttctcaatcg
      901 ctgccgcgtc tcggaacagc acgccatggg tcgcaccgcg cccggtggcc accatgatcg
      961 acatcggcgt tgccaagccc agagcgcaag ggcaggcgat gatcagcacc gacaccgcat
     1021 tgatcaggcc gaagacccaa ctgggctggg gtccgaagag accccaggca aagaacgtca
     1081 gcagggcggc cgccacgacg ccatagacga agtagccagc cacggagtcc gccatgcgtt
     1141 gcatcggggc gcgcgagcgc tgggcgagcg cgaccatctg cacgatctga gacagcaccg
     1201 tggccgaccc gacgtgctcg gactgcatca ccagcgcgcc gctggtgttg agcgtggcac
     1261 cgatcagctt gtcgccaacg cgcttggtca ctggcagggg ctcgcccgtc agcatcgact
     1321 cgtcgagcgc gctgctgccc tcgaccacga caccgtcgac aggcaccttt tcgccgggcc
     1381 gcacgcgcag cttgtcgccg acgtgtacgt tcgccagcgg aatgtcctct tccgagccat
     1441 ccgccgcgat gcgccgcgcg gtcttgggcg acaggcccag cagcgacttg atcgccgccg
     1501 aggtctgcga gcgcgccttc agctccagca gttggcccag cagcgtcagc gagatgatca
     1561 ccgccgcagc ctcgaagtag acagcgacgc ggcccatcga gacgaaggag tccgggaaca
     1621 cctgcggcgc gatcgtcgcc acgatgctgt agacgaaagc cgagccggtg cccagactga
     1681 tcagcgtcca catgttgggg ctgcggttga cgaccgactg cgccccgcgc acgaagaagg
     1741 gtgcgccggc ccacagcacg atcggcagcg tcaggaccaa ctcgatccag ctctgccggg
     1801 ccatgtcgaa ccaccccagg cgatggccga acatggcgag caccgtgacg accacggtca
     1861 ggggcagcgt ccaccagaag cggcgcttga agtctgcgag ttccgggttc tcgccttcat
     1921 cgagtgccgg cagtacgggc tccagcgtca tgccgcactt ggggcagttg cccggatggt
     1981 cctgacggat ctccgggtgc atcgggcagg tgtagatcgt ccccgccgct gcctcgacgg
     2041 cgggtggctc cgacggcaca ggcggcgcgg gattggcgta ccgcgcaggt tccgcggtga
     2101 acttcgtcag gcacttggcg ctgcagaagt agtaaggccg gccttcgtgc tcggcgcggt
     2161 gcggcgattg ctcggtgacc gtcatgccgc acaccggatc cttcaaagcc gccgcggtgg
     2221 cggcgggtgc cg
//
LOCUS       DAOORV010000322         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_72813_length_1960_cov_7.576903, whole genome shotgun sequence.
ACCESSION   DAOORV010000322 DAOORV010000000
VERSION     DAOORV010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72813_length_1960_cov_7.576903"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>1960)
                     /locus_tag="PKV56_19840"
     CDS             complement(145..>1960)
                     /locus_tag="PKV56_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOX70097.1"
                     /translation="FAKYLRDALPHATYLGFTGTPIEKGDVNTPAVFGNYVDIYDIAQ
                     AVEDGATVRIFYESRLAKVSLSEQGRQLIADLDDELEQDELTETQKAKAKWTRMEALI
                     GSEQRVQNIARDIVAHFEARQEVFAGKAMVVSMSRRIAAELYEEIKKLRPGWHDPDLR
                     KGAMKVVMTASSADGPKLAAHHTTKEQRKLLAERMKDDADALKLVIVRDMWLTGFDAP
                     GMHTLYIDKPMKGHNLMQAIARVNRVHKDKPGGLVVDYLGIAADLKAALSFYTDAGGK
                     GDPVLAQEQAVGLLLEKVEVVSALYHGFAYEHFFEADTGEKLSLILQAEEHILGLENG
                     RKRYIDEVSALSQAFAIAVPHEQAMGVRDEVGFFQAVKARLVKFDSPTNSRSSEELET
                     TIRQVIDQALVSEQVIDVFDAAGIKKPDISILSDDFLAELKGYEHKNVALEVLKKLLA
                     DELKVRARRNLVQSRSLLDMLDAAIRKYQNKILTAAEVMDELIKLSKEIVAADDEAKE
                     LGLSPFEYAFYTAVAHNDSARELMQKDQLRELAVVLTQRVRENASIDWTIKENVRAKL
                     KVMVKRTLRQFGYPPDMQLLATDTVLKQAEMLADELTH"
BASE COUNT          299 a          662 c          583 g          416 t
ORIGIN      
        1 gcgctggctg gaactgcagt ccgagatcga ggcgatcact ggcgaggcgg gctgaggcac
       61 gcagcccgca acccagccgc gggtcgcgtg acttgacaca ccgtcacgct gacgctctgg
      121 gtcgtgcctg catgggtcaa ttcctcagtg cgtcagttca tccgccagca tctcggcctg
      181 cttcagcacc gtgtctgtcg ccagcagttg catatccggc ggatagccga actggcgcag
      241 ggtgcgcttg accatcacct tcagcttcgc gcgcacgttc tccttgatcg tccaatcgat
      301 gctggcgttc tctcgcacgc gttgggtcag caccaccgcc agttcccgca gttgatcctt
      361 ctgcatcagc tcgcgcgcgc tgtcgttatg ggccacagcg gtatagaagg cgtattcgaa
      421 ggggctcagg cccagttcct tagcctcgtc gtcggccgcc acgatctctt tgctgagctt
      481 gatcagctcg tccatcacct cggccgccgt caggatcttg ttctggtact ttcggatcgc
      541 ggcatccagc atgtccagca gcgaacggct ctgcaccagg ttgcgccggg cgcgcacctt
      601 caactcatcg gccagcagct tcttcagcac ctccagcgcc acgttcttgt gctcgtagcc
      661 cttcagctct gccaggaagt cgtccgagag gatggagatg tcgggcttct tgatgccggc
      721 cgcgtcgaag acgtcgatca cctgctctga caccaacgcc tggtcgatca cctggcggat
      781 ggtggtctca agttcctcgc tgctgcggct gttggtcggg ctgtcgaact tcaccagcct
      841 tgccttcacc gcctggaaga agcccacctc gtcccgcact cccatggcct gctcatgcgg
      901 cactgcgatg gcgaaggcct gtgacagcgc actcacctcg tcgatgtagc gcttgcgccc
      961 gttttccagg cccaggatgt gctcttccgc ctgcaggatg agagacagct tctcgccggt
     1021 gtcggcctcg aagaaatgtt cataggcaaa gccgtggtac agggccgaga ccacctccac
     1081 cttctccagc agcaggccca ccgcctgctc ctgcgccagc accgggtcgc ccttgccacc
     1141 ggcgtcggtg tagaacgaca gcgccgcctt gaggtcggcc gcaatgccca ggtagtccac
     1201 caccaggccg cccggcttgt ccttgtgcac gcggttcaca cgcgcaatgg cctgcatgag
     1261 gttgtggccc ttcatcggct tgtcgatgta gagcgtgtgc atgcccgggg cgtcaaaacc
     1321 cgtgagccac atgtcgcgca cgatcaccag cttcagcgca tcggcatcgt ccttcatgcg
     1381 ctcggcgagc agtttgcgtt gctccttggt ggtgtggtgc gcggccagct tgggcccgtc
     1441 cgcagacgag gccgtcatca ccaccttcat ggcccccttg cgcaggtcgg ggtcgtgcca
     1501 gccaggtcgc agcttcttga tttcttcata gagctcggcc gcgatgcggc gcgacatgct
     1561 caccaccatg gccttgccgg cgaacacttc ctgccgcgcc tcgaagtggg ccacgatgtc
     1621 gcgcgcgatg ttctgcacgc gctgctcgct gccaatcagc gcttccatcc gggtccactt
     1681 ggccttggcc ttctgcgtct cggtcagctc gtcctgttcc agttcatcgt ccagatcggc
     1741 gatgagctgg cggccctgtt cgctcagact caccttggcc aggcggcttt catagaagat
     1801 gcgcaccgtg gcgccgtctt ccaccgcctg cgcgatgtcg tagatgtcca cgtagttgcc
     1861 gaacaccgcg ggggtgttga cgtcgccctt ctcgatgggc gtgccggtga accccaggta
     1921 ggtggcgtgg ggcaaggcat cgcgcaggta cttggcgaaa
//
LOCUS       DAOORV010000323         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate AMR_MDS_2536
            NODE_106777_length_1541_cov_8.902423, whole genome shotgun
            sequence.
ACCESSION   DAOORV010000323 DAOORV010000000
VERSION     DAOORV010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864611
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (wastewater metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:28:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,925
            Genes (coding)                    :: 3,885
            CDSs (with protein)               :: 3,885
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 7 of 40
            Pseudo Genes (incomplete)         :: 31 of 40
            Pseudo Genes (internal stop)      :: 9 of 40
            Pseudo Genes (multiple problems)  :: 7 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106777_length_1541_cov_8.902423"
                     /isolate="AMR_MDS_2536"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKV56_19845"
     CDS             <1..794
                     /locus_tag="PKV56_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX70098.1"
                     /translation="AKFIAQVMSGDKGIRSVEDVELATLTYAEVKALASGNPKIIEKA
                     GVDAEIAKYASLLSVWRNQLYANESEVASLPMRIESNERLLAALESDAERAHAVLGSD
                     LMVNVHGRTYRGREEIGEVLRATVRSARASISRSARDEVIGTVGAFDLCVFIGRAEDE
                     VHLFVRGASSHECRPCQTGPALHAALVETIREISPHRDDCAQRLASMRSKLEGLTAEL
                     RRPFEHEDRLAALIARQRELEAELDLGKDEVGSNAVEDVPEQIAA"
     gene            complement(863..>1541)
                     /locus_tag="PKV56_19850"
     CDS             complement(863..>1541)
                     /locus_tag="PKV56_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX70099.1"
                     /translation="RGREVEFVQFAVKKSARRPERIAPMPPDVTNILRGGKLGIRDTV
                     IEELERAYGVVAVTEGLERYERFLSGADRPRIGNAGAYLRTIIANKAREEAPAGQGAG
                     PERTALVVVENKTAPAVPASRRDVEDAERHRRFSLIRDQFAKLDQEEQLEWVRRVRDE
                     ADASTAPSPSIMKRLARGEWQSPLVQVMVIQHYARSELGEDWATLPLAEFENLMSIDA
                     GSERDVG"
BASE COUNT          263 a          496 c          502 g          280 t
ORIGIN      
        1 aggccaagtt catcgcgcag gtgatgagcg gggacaaggg catccggtct gtcgaagacg
       61 tcgagctcgc aacgctgacc tacgcggaag tgaaggcgct tgcgtcgggc aatccgaaga
      121 tcatcgagaa agccggtgtc gacgcggaga tcgcgaagta cgcctcgctg ctgtctgtgt
      181 ggcgcaacca gctctacgcg aacgaatctg aggtggccag cttgccgatg cgcatcgaga
      241 gcaatgagcg gctgctcgca gcccttgaat cagatgccga acgggcgcat gcggttctcg
      301 gatcggacct gatggtcaac gtccacggac gcacctaccg cggtcgtgaa gagatcggcg
      361 aagtgcttcg tgcgaccgtg cgcagcgcga gggcatcgat ctcgcggtct gcgcgggacg
      421 aggtcatcgg caccgtaggg gccttcgacc tgtgcgtgtt catcggacgc gcagaggacg
      481 aggtgcatct gttcgtgcgg ggcgcgagct cacacgaatg cagaccgtgc cagaccggac
      541 cggcactcca cgccgccttg gtcgagacga tccgtgagat ttcgccgcac cgcgatgact
      601 gcgcccagcg gctcgcgagt atgcgatcga agctggaagg cctcactgct gaactgcggc
      661 ggccgttcga gcacgaggac cgcctggcgg ctctgatcgc gaggcagcgt gagctcgaag
      721 ccgagttgga cctcggcaag gacgaagtcg gcagcaatgc tgtggaggat gtcccggagc
      781 aaattgccgc ctagtcgcca agcgccctgc cccactgcac acgcagtggg gctttttttt
      841 ctcgaaccag gtgcggtgag ttctagccca cgtctcgctc gctgccggcg tcgatggaca
      901 tgaggttctc gaactcagcc aacggcagcg tcgcccagtc ctcgcccagt tcgctcctcg
      961 cgtagtgctg gatcaccatc acctgaacca gcggcgactg ccactcaccg cgggcgagcc
     1021 gcttcatgat gctggggctg ggtgccgttg aagcgtcggc ctcgtcccgc acgcgcctga
     1081 cccattccaa ttgctcctct tggtccagct tggcgaactg gtcgcggatc aggctgaagc
     1141 gccggtgccg ttcagcgtcc tcgacatcac gtcgtgaggc gggcaccgca ggagcggtct
     1201 tgttttccac cacgacaagg gccgtcctct cgggaccggc gccctgccct gccggcgctt
     1261 cctcgcgcgc cttgttcgcg atgatcgttc gaaggtaagc ccctgcattg ccgatacgcg
     1321 gacgatccgc accgctgaga aatcgctcgt agcgttcgag cccttcggtc acggcgacca
     1381 cgccgtaggc acgctcgagt tcctcgatga ccgtatcgcg aatgcccagc ttgccgcctc
     1441 tcaggatgtt ggtcacgtcg ggcggcatgg gcgcgatccg ctccgggcgt cgcgcgctct
     1501 tcttgacggc gaactggacg aactcgactt ctcggccgcg c
//
